LOCUS       DAEBXN010000001        88716 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.001, whole genome shotgun sequence.
ACCESSION   DAEBXN010000001 DAEBXN010000000
VERSION     DAEBXN010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 88716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 88716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..88716
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.001"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(90..1340)
                     /gene="icd"
                     /locus_tag="KJL99_000001"
     CDS             complement(90..1340)
                     /gene="icd"
                     /locus_tag="KJL99_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HBD4434900.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            1512..2165
                     /gene="rluE"
                     /locus_tag="KJL99_000002"
     CDS             1512..2165
                     /gene="rluE"
                     /locus_tag="KJL99_000002"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HBD4434901.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKHENQPTRVILF
                     NKPYDVLPQFTDETGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            2175..2636
                     /gene="nudJ"
                     /locus_tag="KJL99_000003"
     CDS             2175..2636
                     /gene="nudJ"
                     /locus_tag="KJL99_000003"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HBD4434902.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2690..3796
                     /gene="mnmA"
                     /locus_tag="KJL99_000004"
     CDS             2690..3796
                     /gene="mnmA"
                     /locus_tag="KJL99_000004"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HBD4434903.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIKVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3832..4473
                     /gene="hflD"
                     /locus_tag="KJL99_000005"
     CDS             3832..4473
                     /gene="hflD"
                     /locus_tag="KJL99_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HBD4434904.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSCQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4477..5847
                     /gene="purB"
                     /locus_tag="KJL99_000006"
     CDS             4477..5847
                     /gene="purB"
                     /locus_tag="KJL99_000006"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HBD4434905.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQHRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            6016..6687
                     /gene="phoP"
                     /locus_tag="KJL99_000007"
     CDS             6016..6687
                     /gene="phoP"
                     /locus_tag="KJL99_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HBD4434906.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6687..8147
                     /gene="phoQ"
                     /locus_tag="KJL99_000008"
     CDS             6687..8147
                     /gene="phoQ"
                     /locus_tag="KJL99_000008"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HBD4434907.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAQWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(8944..9225)
                     /locus_tag="KJL99_000009"
     CDS             complement(8944..9225)
                     /locus_tag="KJL99_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4434908.1"
                     /translation="MTEAEILRLIRRVSGISQQADEQTTQPDSVTAENYARVVAEVMR
                     RDGIQLNDADMRDIRIRVLEMLAYNRRVELYREKEKITYHWKKPERLRR"
     gene            complement(9239..10900)
                     /locus_tag="KJL99_000010"
     CDS             complement(9239..10900)
                     /locus_tag="KJL99_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBD4434909.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYTFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDNARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLISRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQAEHEDFAFDISDSHRQKLEEFSGV"
     gene            complement(10884..11210)
                     /locus_tag="KJL99_000011"
     CDS             complement(10884..11210)
                     /locus_tag="KJL99_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HBD4434910.1"
                     /translation="MDDIAVKQWREKSRQLAERGDLTPADWSNLELYCVNYSIYRKAV
                     ADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRRKNPPETEEED
                     ELDRLE"
     gene            complement(11266..11448)
                     /locus_tag="KJL99_000012"
     CDS             complement(11266..11448)
                     /locus_tag="KJL99_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4434911.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            complement(11432..11872)
                     /locus_tag="KJL99_000013"
     CDS             complement(11432..11872)
                     /locus_tag="KJL99_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBD4434912.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            complement(11872..12168)
                     /locus_tag="KJL99_000014"
     CDS             complement(11872..12168)
                     /locus_tag="KJL99_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HBD4434913.1"
                     /translation="MTEELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRR
                     FDDGLAFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGGY"
     gene            complement(12165..12503)
                     /locus_tag="KJL99_000015"
     CDS             complement(12165..12503)
                     /locus_tag="KJL99_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HBD4434914.1"
                     /translation="MNIGRLRDRITIQTLKQTRDITGEILETWEDGHTLWASVNMVSS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            complement(12500..13711)
                     /locus_tag="KJL99_000016"
     CDS             complement(12500..13711)
                     /locus_tag="KJL99_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBD4434915.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISDAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRYCLLSGNAYAVIQWGRDGFPAALHPYPPQSVNVEQTGDHNWRYCITDAYTGNIRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSDVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            complement(13713..14285)
                     /locus_tag="KJL99_000017"
     CDS             complement(13713..14285)
                     /locus_tag="KJL99_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HBD4434916.1"
                     /translation="MGEKIMKSMEIRSSEITTSGAGTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFGFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPRADNRRWWADLAGV"
     gene            complement(14325..15482)
                     /locus_tag="KJL99_000018"
                     /pseudo
     CDS             complement(14325..15482)
                     /locus_tag="KJL99_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
     gene            complement(15783..16007)
                     /locus_tag="KJL99_000019"
     CDS             complement(15783..16007)
                     /locus_tag="KJL99_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4434917.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMNITDYRAFLETGALIEKDHHG
                     FVRCALSGEMLAVNPEQTDALIEFLKGIRD"
     gene            complement(16149..16310)
                     /locus_tag="KJL99_000020"
     CDS             complement(16149..16310)
                     /locus_tag="KJL99_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HBD4434918.1"
                     /translation="MTAQIAAYGQLVGDPLVKQSSKGTPEPPTENYADLRNAVNRTYA
                     DMGLSKLAE"
     gene            complement(16320..16625)
                     /locus_tag="KJL99_000021"
     CDS             complement(16320..16625)
                     /locus_tag="KJL99_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4434919.1"
                     /translation="MSEPKFGEKFYKHNGRITILQISAATPGWWVETDEGSSPVASWE
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            complement(16622..16900)
                     /locus_tag="KJL99_000022"
     CDS             complement(16622..16900)
                     /locus_tag="KJL99_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4434920.1"
                     /translation="MHTSGKFNKSFKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            complement(17189..18937)
                     /locus_tag="KJL99_000023"
     CDS             complement(17189..18937)
                     /locus_tag="KJL99_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBD4434921.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWSHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIGQKLAAADIQDA
                     NFYPEGMHVQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQLLAERFDG
                     VCVHQESEIVHVWRGGVWCPVSTMELSREMVVIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNALNFH
                     KWLEHAAGKDQRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLAGK
                     QNTVSAEMTSLDDAGGRAQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKYEK
                     RFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAEIP
                     VIIRRLLANFTDSEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKVKG
                     VTQTNAITTDECDAFL"
     gene            complement(18934..19233)
                     /locus_tag="KJL99_000024"
     CDS             complement(18934..19233)
                     /locus_tag="KJL99_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HBD4434922.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGMVTIKGVAERRITY
                     RREGYEYDCVMPVYQFRRDFTLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            complement(19239..19472)
                     /locus_tag="KJL99_000025"
     CDS             complement(19239..19472)
                     /locus_tag="KJL99_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4434923.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            complement(19474..19694)
                     /locus_tag="KJL99_000026"
                     /pseudo
     CDS             complement(19474..19694)
                     /locus_tag="KJL99_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(19687..19908)
                     /locus_tag="KJL99_000027"
     CDS             complement(19687..19908)
                     /locus_tag="KJL99_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4434924.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            complement(19898..20098)
                     /locus_tag="KJL99_000028"
     CDS             complement(19898..20098)
                     /locus_tag="KJL99_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HBD4434925.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            20228..20407
                     /locus_tag="KJL99_000029"
     CDS             20228..20407
                     /locus_tag="KJL99_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4434926.1"
                     /translation="MSTHKNERRGNPPFQFRLDPDLREKMEKAQQQDGDDSLAAWIKR
                     IIRKELQQRGIEPKG"
     gene            20532..20861
                     /locus_tag="KJL99_000030"
     CDS             20532..20861
                     /locus_tag="KJL99_000030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4434927.1"
                     /translation="MNQNDSRRVLLPLRSTLKNTHQRRELRSYGNALNQTDCIAHQPD
                     SGYHPRAYVWDHTHKRRSGLSVQKGGSISGPLFLCLKNPNFVVFQFHQGERIPAHVLA
                     YIQSSWL"
     gene            complement(20911..21099)
                     /locus_tag="KJL99_000031"
     CDS             complement(20911..21099)
                     /locus_tag="KJL99_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HBD4434928.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(21474..22703)
                     /locus_tag="KJL99_000032"
     CDS             complement(21474..22703)
                     /locus_tag="KJL99_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBD4434929.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLSLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYIALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQETSVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            22952..24073
                     /gene="roxA"
                     /locus_tag="KJL99_000033"
                     /gene_synonym="ycfD"
     CDS             22952..24073
                     /gene="roxA"
                     /locus_tag="KJL99_000033"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HBD4434930.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(24122..25348)
                     /gene="pepT"
                     /locus_tag="KJL99_000034"
     CDS             complement(24122..25348)
                     /gene="pepT"
                     /locus_tag="KJL99_000034"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HBD4434931.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVILEGMEK
                     AVQVIVRIAELTAQRK"
     gene            25598..26734
                     /gene="potA"
                     /locus_tag="KJL99_000035"
     CDS             25598..26734
                     /gene="potA"
                     /locus_tag="KJL99_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HBD4434932.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            26718..27575
                     /gene="potB"
                     /locus_tag="KJL99_000036"
     CDS             26718..27575
                     /gene="potB"
                     /locus_tag="KJL99_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HBD4434933.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            27572..28366
                     /gene="potC"
                     /locus_tag="KJL99_000037"
     CDS             27572..28366
                     /gene="potC"
                     /locus_tag="KJL99_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HBD4434934.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            28363..29409
                     /gene="potD"
                     /locus_tag="KJL99_000038"
     CDS             28363..29409
                     /gene="potD"
                     /locus_tag="KJL99_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HBD4434935.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(29565..30386)
                     /gene="cobB"
                     /locus_tag="KJL99_000039"
     CDS             complement(29565..30386)
                     /gene="cobB"
                     /locus_tag="KJL99_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HBD4434936.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADVFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLEGL"
     gene            complement(30402..31313)
                     /gene="nagK"
                     /locus_tag="KJL99_000040"
     CDS             complement(30402..31313)
                     /gene="nagK"
                     /locus_tag="KJL99_000040"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HBD4434937.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGKLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLVFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVSLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPCHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(31342..32586)
                     /gene="lolE"
                     /locus_tag="KJL99_000041"
     CDS             complement(31342..32586)
                     /gene="lolE"
                     /locus_tag="KJL99_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HBD4434938.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(32586..33287)
                     /gene="lolD"
                     /locus_tag="KJL99_000042"
     CDS             complement(32586..33287)
                     /gene="lolD"
                     /locus_tag="KJL99_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HBD4434939.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLLNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSCALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(33280..34479)
                     /gene="lolC"
                     /locus_tag="KJL99_000043"
     CDS             complement(33280..34479)
                     /gene="lolC"
                     /locus_tag="KJL99_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HBD4434940.1"
                     /translation="MYQPVALFIGLRYMRGRAVDRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAAN
                     ITGWRLWLDEPLRVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            34741..35814
                     /locus_tag="KJL99_000044"
     CDS             34741..35814
                     /locus_tag="KJL99_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HBD4434941.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            35942..39388
                     /gene="mfd"
                     /locus_tag="KJL99_000045"
     CDS             35942..39388
                     /gene="mfd"
                     /locus_tag="KJL99_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HBD4434942.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGILIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKARNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            39531..40493
                     /gene="ldtC"
                     /locus_tag="KJL99_000046"
     CDS             39531..40493
                     /gene="ldtC"
                     /locus_tag="KJL99_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HBD4434943.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPASSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(40576..40788)
                     /locus_tag="KJL99_000047"
     CDS             complement(40576..40788)
                     /locus_tag="KJL99_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015705050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBD4434944.1"
                     /translation="MSFANFAAVEVQSTPEGQQKVGTISANAGTNLGSLEEQLAQKAD
                     EMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            41073..41705
                     /gene="comR"
                     /locus_tag="KJL99_000048"
     CDS             41073..41705
                     /gene="comR"
                     /locus_tag="KJL99_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HBD4434945.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(41767..42306)
                     /locus_tag="KJL99_000049"
     CDS             complement(41767..42306)
                     /locus_tag="KJL99_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HBD4434946.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(42533..43837)
                     /gene="ndh"
                     /locus_tag="KJL99_000050"
     CDS             complement(42533..43837)
                     /gene="ndh"
                     /locus_tag="KJL99_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HBD4434947.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAIKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(44245..44787)
                     /locus_tag="KJL99_000051"
     CDS             complement(44245..44787)
                     /locus_tag="KJL99_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBD4434948.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(44810..45835)
                     /gene="nagZ"
                     /locus_tag="KJL99_000052"
     CDS             complement(44810..45835)
                     /gene="nagZ"
                     /locus_tag="KJL99_000052"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HBD4434949.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKVGH"
     gene            complement(45846..46670)
                     /gene="thiK"
                     /locus_tag="KJL99_000053"
     CDS             complement(45846..46670)
                     /gene="thiK"
                     /locus_tag="KJL99_000053"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HBD4434950.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(46651..47292)
                     /gene="lpoB"
                     /locus_tag="KJL99_000054"
     CDS             complement(46651..47292)
                     /gene="lpoB"
                     /locus_tag="KJL99_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HBD4434951.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSCASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(47307..47684)
                     /locus_tag="KJL99_000055"
     CDS             complement(47307..47684)
                     /locus_tag="KJL99_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HBD4434952.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTIYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(47687..48046)
                     /gene="hinT"
                     /locus_tag="KJL99_000056"
     CDS             complement(47687..48046)
                     /gene="hinT"
                     /locus_tag="KJL99_000056"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HBD4434953.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            48177..48358
                     /locus_tag="KJL99_000057"
                     /pseudo
     CDS             48177..48358
                     /locus_tag="KJL99_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012138106.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            48391..50576
                     /gene="fhuE"
                     /locus_tag="KJL99_000058"
                     /pseudo
     CDS             48391..50576
                     /gene="fhuE"
                     /locus_tag="KJL99_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(50636..52069)
                     /gene="ptsG"
                     /locus_tag="KJL99_000059"
     CDS             complement(50636..52069)
                     /gene="ptsG"
                     /locus_tag="KJL99_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HBD4434954.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFFFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLLPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(52364..53161)
                     /locus_tag="KJL99_000060"
     CDS             complement(52364..53161)
                     /locus_tag="KJL99_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBD4434955.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(53172..54176)
                     /gene="holB"
                     /locus_tag="KJL99_000061"
     CDS             complement(53172..54176)
                     /gene="holB"
                     /locus_tag="KJL99_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HBD4434956.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSCLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(54173..54814)
                     /locus_tag="KJL99_000062"
     CDS             complement(54173..54814)
                     /locus_tag="KJL99_000062"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HBD4434957.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(54804..55826)
                     /gene="yceG"
                     /locus_tag="KJL99_000063"
     CDS             complement(54804..55826)
                     /gene="yceG"
                     /locus_tag="KJL99_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HBD4434958.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTLMYTANTTDVALLKRVHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(55829..56638)
                     /gene="pabC"
                     /locus_tag="KJL99_000064"
     CDS             complement(55829..56638)
                     /gene="pabC"
                     /locus_tag="KJL99_000064"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HBD4434959.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIL
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVTFSSATLYEYLAPLCERPN"
     gene            complement(56758..57999)
                     /gene="fabF"
                     /locus_tag="KJL99_000065"
     CDS             complement(56758..57999)
                     /gene="fabF"
                     /locus_tag="KJL99_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HBD4434960.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(58086..58322)
                     /gene="acpP"
                     /locus_tag="KJL99_000066"
     CDS             complement(58086..58322)
                     /gene="acpP"
                     /locus_tag="KJL99_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBD4434961.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(58533..59267)
                     /gene="fabG"
                     /locus_tag="KJL99_000067"
     CDS             complement(58533..59267)
                     /gene="fabG"
                     /locus_tag="KJL99_000067"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HBD4434962.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(59280..60209)
                     /gene="fabD"
                     /locus_tag="KJL99_000068"
     CDS             complement(59280..60209)
                     /gene="fabD"
                     /locus_tag="KJL99_000068"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBD4434963.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGSAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDAPIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(60225..61178)
                     /gene="fabH"
                     /locus_tag="KJL99_000069"
     CDS             complement(60225..61178)
                     /gene="fabH"
                     /locus_tag="KJL99_000069"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HBD4434964.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLDIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(61246..62316)
                     /gene="plsX"
                     /locus_tag="KJL99_000070"
     CDS             complement(61246..62316)
                     /gene="plsX"
                     /locus_tag="KJL99_000070"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HBD4434965.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(62397..62570)
                     /gene="rpmF"
                     /locus_tag="KJL99_000071"
     CDS             complement(62397..62570)
                     /gene="rpmF"
                     /locus_tag="KJL99_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBD4434966.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(62622..63143)
                     /gene="yceD"
                     /locus_tag="KJL99_000072"
     CDS             complement(62622..63143)
                     /gene="yceD"
                     /locus_tag="KJL99_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HBD4434967.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            63342..63926
                     /gene="yceF"
                     /locus_tag="KJL99_000073"
     CDS             63342..63926
                     /gene="yceF"
                     /locus_tag="KJL99_000073"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HBD4434968.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKSQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(64038..64997)
                     /gene="rluC"
                     /locus_tag="KJL99_000074"
     CDS             complement(64038..64997)
                     /gene="rluC"
                     /locus_tag="KJL99_000074"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HBD4434969.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            65570..68761
                     /gene="rne"
                     /locus_tag="KJL99_000075"
     CDS             65570..68761
                     /gene="rne"
                     /locus_tag="KJL99_000075"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HBD4434970.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPMVEPQEETADADIEEVAETAEVVVAE
                     PEVVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDW
                     QRPTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(68958..69900)
                     /gene="flgL"
                     /locus_tag="KJL99_000076"
                     /pseudo
     CDS             complement(68958..69900)
                     /gene="flgL"
                     /locus_tag="KJL99_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
     gene            complement(69912..71554)
                     /gene="flgK"
                     /locus_tag="KJL99_000077"
                     /pseudo
     CDS             complement(69912..71554)
                     /gene="flgK"
                     /locus_tag="KJL99_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(71620..72561)
                     /gene="flgJ"
                     /locus_tag="KJL99_000078"
     CDS             complement(71620..72561)
                     /gene="flgJ"
                     /locus_tag="KJL99_000078"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HBD4434971.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSRAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGQVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(72561..73658)
                     /gene="flgI"
                     /locus_tag="KJL99_000079"
     CDS             complement(72561..73658)
                     /gene="flgI"
                     /locus_tag="KJL99_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HBD4434972.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTVSLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(73670..74368)
                     /gene="flgH"
                     /locus_tag="KJL99_000080"
     CDS             complement(73670..74368)
                     /gene="flgH"
                     /locus_tag="KJL99_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HBD4434973.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(74421..75203)
                     /gene="flgG"
                     /locus_tag="KJL99_000081"
     CDS             complement(74421..75203)
                     /gene="flgG"
                     /locus_tag="KJL99_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HBD4434974.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYIRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(75341..76092)
                     /gene="flgF"
                     /locus_tag="KJL99_000082"
                     /pseudo
     CDS             complement(75341..76092)
                     /gene="flgF"
                     /locus_tag="KJL99_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
     gene            complement(76112..77320)
                     /gene="flgE"
                     /locus_tag="KJL99_000083"
     CDS             complement(76112..77320)
                     /gene="flgE"
                     /locus_tag="KJL99_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HBD4434975.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLLNTQGLQLTGYPVTGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTLVDGAMANNIATGAINGAEPATFSLSFLNSMQQNTGANNIV
                     ATTQNGYKPGDLVSYQINDDGTVVGNNSNEQTQLLGQIVLANFANNEGLASEGDNVWS
                     ATQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYKSNAQTIKTQD
                     QILNTRVNLR"
     gene            complement(77345..78040)
                     /gene="flgD"
                     /locus_tag="KJL99_000084"
     CDS             complement(77345..78040)
                     /gene="flgD"
                     /locus_tag="KJL99_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HBD4434976.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGSLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(78052..78455)
                     /gene="flgC"
                     /locus_tag="KJL99_000085"
                     /pseudo
     CDS             complement(78052..78455)
                     /gene="flgC"
                     /locus_tag="KJL99_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
     gene            complement(78459..78875)
                     /gene="flgB"
                     /locus_tag="KJL99_000086"
     CDS             complement(78459..78875)
                     /gene="flgB"
                     /locus_tag="KJL99_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HBD4434977.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            79030..79689
                     /gene="flgA"
                     /locus_tag="KJL99_000087"
     CDS             79030..79689
                     /gene="flgA"
                     /locus_tag="KJL99_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HBD4434978.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSEQVVSGVVDADGNILIN
                     L"
     gene            79765..80058
                     /gene="flgM"
                     /locus_tag="KJL99_000088"
     CDS             79765..80058
                     /gene="flgM"
                     /locus_tag="KJL99_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HBD4434979.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            80063..80479
                     /gene="flgN"
                     /locus_tag="KJL99_000089"
     CDS             80063..80479
                     /gene="flgN"
                     /locus_tag="KJL99_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HBD4434980.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPHRGSKKISI"
     gene            complement(80519..82054)
                     /gene="murJ"
                     /locus_tag="KJL99_000090"
     CDS             complement(80519..82054)
                     /gene="murJ"
                     /locus_tag="KJL99_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HBD4434981.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(82254..83087)
                     /locus_tag="KJL99_000091"
     CDS             complement(82254..83087)
                     /locus_tag="KJL99_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBD4434982.1"
                     /translation="MKKLRIGVVGLGGIVQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICDSWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     V"
     gene            complement(83089..83736)
                     /locus_tag="KJL99_000092"
     CDS             complement(83089..83736)
                     /locus_tag="KJL99_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HBD4434983.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTAMSNMVDGDLQTRVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(83747..84331)
                     /gene="rimJ"
                     /locus_tag="KJL99_000093"
     CDS             complement(83747..84331)
                     /gene="rimJ"
                     /locus_tag="KJL99_000093"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HBD4434984.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            84567..85775
                     /gene="mdtH"
                     /locus_tag="KJL99_000094"
     CDS             84567..85775
                     /gene="mdtH"
                     /locus_tag="KJL99_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HBD4434985.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSASAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            85839..86486
                     /gene="grxB"
                     /locus_tag="KJL99_000095"
     CDS             85839..86486
                     /gene="grxB"
                     /locus_tag="KJL99_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HBD4434986.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYVNKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVANYRDNMAKQTQINLLSSMAI"
     gene            86620..87180
                     /locus_tag="KJL99_000096"
     CDS             86620..87180
                     /locus_tag="KJL99_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBD4434987.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            87286..88332
                     /gene="pyrC"
                     /locus_tag="KJL99_000097"
     CDS             87286..88332
                     /gene="pyrC"
                     /locus_tag="KJL99_000097"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HBD4434988.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEAVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            88406..88651
                     /gene="dinI"
                     /locus_tag="KJL99_000098"
     CDS             88406..88651
                     /gene="dinI"
                     /locus_tag="KJL99_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HBD4434989.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
BASE COUNT        21219 a        23688 c        21957 g        21852 t
ORIGIN      
        1 aatatgctcg atatgctatt aataatatcc acataaataa aacaacgggc gtgttatacg
       61 cccgtttaaa tatttaacac atgtagtgat tacatattct tgatgatcgc atcaccaaac
      121 tctgaacatt tcagcagttt agcgccttcc atcagacgct cgaagtcata ggttacggtc
      181 ttggcgttga tcgcgccttc cataccttta acaatcaggt ctgcggcttc agtccagccc
      241 atatggcgta acatcatctc agcggagaga ataatagagc cagggtttac tttgtcctga
      301 ccggcgtatt tcggcgcagt accgtgggtg gcttcaaaca gggcgcattc gtcaccgatg
      361 tttgctccag gggcgatacc gataccgcca acctgcgctg ccagagcgtc agaaatgtag
      421 tcaccgttca ggttcataca ggcgataaca tcatattcag ccggacgcag caggatctgt
      481 tgcaggaatg catcagcaat cacgtcttta atgacgatct ctttgccggt attcgggttt
      541 ttaactttca gccacgggcc gccgtcgatc agttcaccgc caaattcttc acgcgccagc
      601 tggtagcccc agtctttaaa cgcgccttcg gtgaacttca tgatgttgcc tttgtgcacc
      661 agggtcacag agtcacgatc gttagcaatt gcgtattcga tcgctgcacg aaccagacgt
      721 ttggtgcctt cttcagaaca cggcttaata ccgataccgc aatgttccgg gaagcgaatt
      781 ttcttcacgc ccatctcttc acgcaggaat ttaatcactt tctcggcgtc ggcagagtca
      841 gctttccatt cgatacccgc ataaatgtct tccgagtttt cacggaagat aaccatatcg
      901 gtcagttcag ggtgtttaac cgggcttgga gtgccctgat agtaacgtac cggacgcagg
      961 cagatgtaga gatccagttc ctgacgcagg gcaacgttga gagaacgaat accgccacca
     1021 accggagtgg tcagcgggcc tttaatggca acgcgatatt cacgaatcag atcaagggtt
     1081 tcagcaggca gccagacatc ctgaccataa acctgtgtgg atttttcacc ggtgtaaatt
     1141 tccatccagg agattttacg ctcgccttta taggctttct cgactgcagc gtcgaccact
     1201 ttcagcatgg ctggggttac atctacaccg attccatcac cttcaatgta agggataatc
     1261 ggattttcag gaacgttgag tttgccgttt tgcagggtga tcttcttgcc ttgtgccgga
     1321 acaactactt tactttccat tcacctctcc ttcgagcgct actggtttgc tcgtctgcca
     1381 ccacgttgca tatgcgtttg cgtcctgcga tacggatgct ttagagcaat tttttgttaa
     1441 tgatttgtaa ttgacttgtc aatactaccc tattgtttgc cgtcatgaaa gatgcgcgtt
     1501 attaggctat aatgcggcaa ttcataatct ctgaaaatac catgcaaaaa acttctttta
     1561 gaaatcacca ggttaagcga ttcagctcgc aacgttctac caggcgtaaa catgaaaacc
     1621 agcccacgcg tgtgatcctc ttcaataaac cctacgatgt tcttccgcag ttcaccgatg
     1681 aaaccggacg caaaacatta aaagaattca tcccggttca gggcgtttat gcagcaggtc
     1741 gccttgaccg cgatagcgaa gggttgctgg tgctgaccaa taacggcgcg ttgcaggcgc
     1801 gtttaaccca gccgggtaaa cgcaccggaa aaatctatta tgtgcaggtg gaaggtattc
     1861 ccacacaaga cgcacttgaa gccttgcgca atggcgtaac cttaaatgat ggccctaccc
     1921 tgcccgcagg cgcggagctg gttgacgaac ccgcgtggtt atggccgcgg aatccaccaa
     1981 ttcgtgaacg caaaagtatt cccaccagct ggctgaagat caccttatat gaaggacgta
     2041 atcgccaggt gcgccgcatg accgcccatg ttggtttccc cacgctgcga ctgattcgct
     2101 atgcgatggg agattactct ttggataatc ttgccaatgg cgaatggcga gaagtgacag
     2161 attaaggatg caaaatgttt aaaccgcacg ttaccgttgc ttgcgtggtg cacgcagaag
     2221 gcaaattttt agtcgttgaa gagacgatta atggtaaagc gttatggaac caacctgccg
     2281 ggcatctgga agccgatgaa accttagtgg aagccgccgc ccgtgaactg tgggaagaaa
     2341 ccggcatcag cgcgcagccg caacacttta ttcgtatgca tcagtggatt gcgccagata
     2401 aaacgccgtt tttgcgtttc ctctttgcca ttgagcttga gcaaatatgc ccgacccagc
     2461 ctcatgacag cgatatcgac tgctgccgtt gggtcagcgc cgaagaaatt ttacaggcgt
     2521 caaatcttcg ttcgccgctg gtggcggaaa gtattcgttg ttatcaaagc gggcaacgtt
     2581 atccgctgga gatgattggc gattttaact ggccttttac aaagggtgtc atctaaaagg
     2641 ctgcgtgata gaatacgccg ccttgaagtt caatgtcgtg agtgatccaa tgtctgaaac
     2701 cgcaaaaaaa gtaatcgtcg gcatgtccgg cggtgtcgat tcctccgttt ctgcctggct
     2761 gttgcaacaa cagggatatc aggtcgaagg cctgtttatg aagaactggg aagaagacga
     2821 cggtgaggaa tattgcacag cagcagcgga tctggctgat gcccaggcgg tctgcgacaa
     2881 gctcggcatt gaactgcaca ccgttaactt tgctgccgag tactgggaca acgtcttcga
     2941 actgttcctt gccgaatata aagccggtcg cacgccgaat ccggatattc tgtgcaacaa
     3001 ggagatcaaa tttaaagcct tcctcgaatt tgccgccgaa gatttaggtg ccgattatat
     3061 cgctaccggt cattacgtac gtcgggccga tgtcgatggc aagagccgcc tgctgcgtgg
     3121 tctggacagc aataaagacc agagctactt cctttatacg ctcagccatg agcagattgc
     3181 gcaaagcctg ttcccggtag gcgaactgga aaaaccgcag gtgcgtaaga ttgctgaaga
     3241 tcttggtctg gtcaccgcga agaaaaaaga ctctaccggc atttgcttca ttggcgaacg
     3301 taaattccgc gagttcctgg gccgttatct cccggcgcaa ccgggcaaaa tcattaccgt
     3361 cgatggcgat gaaattggtg agcaccaggg gctgatgtat cacactctcg gtcagcgtaa
     3421 aggtctgggt atcggtggca ccaaagaagg taccgaagaa ccgtggtatg tggtggacaa
     3481 agacgtcgaa aacaacattc tggttgtcgc tcagggccat gaacacccgc ggctgatgtc
     3541 tgtcgggttg attgcccagc agctgcactg ggtcgatcgc gaaccattca ccggcactat
     3601 gcgttgcacg gtaaaaaccc gctatcgcca gaccgacatt ccgtgcaccg tcaaggcgct
     3661 ggacgacgat cgcattaaag tgattttcga tgagccggtt gccgccgtga cgccgggcca
     3721 gtctgccgtc ttctataacg gtgaagtgtg cctcggtggc ggtattattg agcagcgtct
     3781 gccgctgccg gtctgattat tatctttact taaaacaagg aagcagtgaa cgtggcaaag
     3841 aattactatg acatcaccct cgccctggcc ggtatttgtc agtcggcacg cctggtgcaa
     3901 caactcgctc accaggggca ttgtgatgcc gatgcgctac acgtctcact caacagtatt
     3961 attgatatga accccagctc gacgctggcg gtttttggcg gtagcgaagc caacctgcgc
     4021 gtcgggctgg aaaccctgct cggcgtgctc aatgccagca gttgccaggg cttaaacgcc
     4081 gaattaaccc gctacacact cagcttgatg gtgcttgagc gcaaactctc ctcagcgaaa
     4141 ggcgcgctcg acactctggg caaccggatc aacggcctgc aacgccagct cgaacacttc
     4201 gatttacagt ccgaaacgct gatgagcgcg atggctgcta tctatgttga tgtgatcagc
     4261 ccgcttggcc cgcgcattca ggtcaccggt tcccctgctg tactgcaaag cccacaagtg
     4321 caggcgaaag ttcgcgcaac cctgctggca ggcattcgcg ccgccgtgct ctggcaccag
     4381 gtcggcggcg gacgtctaca actgatgttt tctcgtaatc gcctgaccac tcaggcaaaa
     4441 caaattcttg ctcatttaac cccggagttg tgatctatgg aattatcctc actgaccgcc
     4501 gtttcccctg tcgatggacg ctacggcgat aaagtcagcg cgctgcgcgg gattttcagc
     4561 gaatatggtt tgctgaaatt ccgtgtacaa gttgaagtac gttggctgca aaaactggcc
     4621 gcgcacgcag cgatcaagga agttcctgct tttgctgccg acgcaatcgg ttaccttgat
     4681 gcaattgtcg ccaatttcag cgaagaagat gccgcacgca tcaaaaccat cgagcgtacc
     4741 actaaccacg acgttaaagc ggttgagtat ttcctgaaag aaaaagtggc ggagatcccg
     4801 gaactgcacg cggtttctga attcatccac tttgcctgta cttcggaaga tatcaataac
     4861 ctctcccacg cattaatgct gaaaaccgcg cgtgatgaag tgatcctgcc gtactggcgt
     4921 caactgattg atggcattaa agatctcgcc gttcagtacc gcgatatccc gctgctgtct
     4981 cgtacccacg gtcagccagc cacgccgtca accatcggta aagagatggc aaacgtcgcc
     5041 taccgtatgg agcgccagca ccgccagctt aaccaggtgg agatcctcgg caaaatcaac
     5101 ggcgcggtcg gtaactataa cgcccacatc gccgcttacc cggaagttga ctggcatcag
     5161 ttcagcgaag agttcgtcac ctcgctgggt attcaatgga acccgtacac tacccagatt
     5221 gaaccgcacg actacattgc cgaactgttt gattgcgttg cgcgcttcaa caccattctg
     5281 atcgactttg accgtgacgt ctggggttat atcgccctta accacttcaa acagaaaacc
     5341 attgctggtg agattggttc ttccaccatg ccgcataaag ttaacccgat cgacttcgaa
     5401 aactccgaag ggaatctggg cctttccaac gcggtattgc agcatctggc aagcaaactg
     5461 ccggtttccc gctggcagcg tgacctgacc gactccaccg tgctgcgtaa cctcggcgtg
     5521 ggtatcggtt atgcgctgat tgcgtatcaa tccaccctga aaggcgtgag caaactggaa
     5581 gtgaaccgtg accatctgct ggatgagctg gatcacaact gggaagtgct ggcagaacca
     5641 atccagacag ttatgcgtcg ctatggcatc gaaaaaccgt acgagaagct gaaagagctt
     5701 actcgcggta agcgcgttga cgccgaaggc atgaagcagt ttatcgatgg tctggcgttg
     5761 ccagaagaag agaaagcccg cctgaaagcg atgacgccgg ctaactatat tggtcgagct
     5821 atcacgatgg ttgatgagct gaaataagcc tcaaatcagt gccggatggc gatgctgtcc
     5881 ggcctgctta ttaagattat ccactttttc ttttttcact ttacctcccc tccccgctgg
     5941 tttatttaat gtttaccccc ataaacacat aatcgcgtta cactatttta ataattaaga
     6001 cagggagaaa taaaaatgcg cgtactggtt gttgaagaca atgcgttgtt acgtcaccac
     6061 cttaaagttc agattcagga tgctggtcat caggtcgatg acgcagaaga tgccaaagaa
     6121 gccgattatt atctcaatga acatttaccg gatattgcga ttgtcgatct cggattgcct
     6181 gacgaggacg gtctgtcact gattcgccgc tggcgtagca acgatgtttc actgccgatt
     6241 ctggtattaa ccgcccgtga aagctggcag gacaaagtcg aagtattaag tgccggtgct
     6301 gatgattacg tgactaaacc gtttcatatt gaagaggtga tggcgcgaat gcaggcatta
     6361 atgcggcgta atagcggtct ggcttcacag gtcatttcgc tccccccgtt tcaggttgat
     6421 ctctctcgcc gtgaattatc tattaatgac gaagtgatca aactgaccgc gttcgaatac
     6481 accattatgg aaacgttgat acgcaataat ggcaaagtgg tcagcaaaga ttcgttaatg
     6541 ctacaactct atccggatgc ggagctgcgg gaaagccata ccattgatgt actgatggga
     6601 cgtctgcgca aaaaaattca ggcacaatat ccgcaagaag tcattaccac cgttcgcggc
     6661 cagggctacc tgttcgaatt gcgctgatga aaaaattact gcgtcttttt ttcccgctct
     6721 cgctgcgggt acgttttctg ttggcaacgg cagcggtagt actggtgctt tcgcttgcct
     6781 acggaatggt cgcgctgatc ggttatagcg tcagtttcga taaaactacg tttcggctgt
     6841 tacgtggcga gagcaatctg ttctataccc ttgcgcagtg ggaaaacaat aagttgcatg
     6901 tcgagttacc cgaaaatatc gacaagcaaa gccccaccat gacgctaatt tatgatgaga
     6961 acgggcagct tttatgggcg caacgtgacg tgccctggct gatgaaaatg atccagcctg
     7021 actggctgaa atcgaatggt tttcatgaaa ttgaagcgga tgttaacgat accagcctct
     7081 tgctgagtgg agatcattcg atacagcaac agttgcagga agtgcgggaa gatgatgacg
     7141 acgcggagat gacccactcg gtggcggtaa acgtctaccc ggcaacatcg cggatgccaa
     7201 aattaaccat tgtggtggtg gataccattc cggtggagct aaaaagttcc tatatggtct
     7261 ggagctggtt tatctatgtg ctctcagcca atctgctgtt agtgatcccg ctgctgtggg
     7321 tcgccgcctg gtggagttta cgccccatcg aagccctggc aaaagaagtc cgcgaactgg
     7381 aagaacataa ccgcgaattg ctcaatccag ccacaacgcg agaactgacc agtctggtac
     7441 gaaacctgaa ccgattgtta aaaagtgaac gcgaacgtta cgacaaatac cgtacgacgc
     7501 tcaccgacct gacccatagt ctgaaaacgc cactggcggt gctgcaaagt acgctgcgtt
     7561 ctctgcgtag tgaaaagatg agcgtcagtg atgctgagcc ggtaatgctg gagcaaatca
     7621 gccgcatttc acagcaaatt ggctactacc tgcatcgtgc cagtatgcgc ggcgggacat
     7681 tgctcagccg cgagctgcat ccggtcgccc cactgctgga caatctcacc tcagcgctga
     7741 acaaagtgta tcaacgcaaa ggggtcaata tctctctcga tatttcgcca gagatcagct
     7801 ttgtcggtga gcagaacgat tttgtcgagg tgatgggcaa cgtgctggat aatgcctgta
     7861 aatattgcct cgagtttgtc gaaatttctg caaggcaaac cgacgagcat ctctatattg
     7921 tggtcgagga tgatggcccc ggtattccat taagcaagcg agaggtcatt ttcgaccgtg
     7981 gtcaacgggt tgatacttta cgccctgggc aaggtgtagg gctggcggta gcccgcgaaa
     8041 tcaccgagca atatgagggt aaaatcgtcg ccggagagag catgctgggc ggtgcgcgga
     8101 tggaggtgat ttttggtcgc cagcattctg cgccgaaaga tgaataaata tgtcctttta
     8161 ccactacatc aagtcattga ttcgatcaaa caattttcat tgaaaaagtg ccaaaaatcg
     8221 caaattgact acaccattaa ctacaccgtt cggtgcactg tatgaaacaa cgtggaacaa
     8281 atagacacaa gaaatataca ggcgggtaat ctttccaggg ggaagcgcac caattcatga
     8341 ggggcgttaa tgtcgatatg gggatcccca taacggggct accggatttt ttttccggtt
     8401 aacctttaat caggctggtg ggttttacat acccttgatc accgtaatga tgatccagat
     8461 gtgcaccttt tccaccaatg gcggaaaagc tccaaccgta acgatgatcg ctatggttgc
     8521 ctcgatggtc tgcgtggtca ttatgaccgt tcagccagtg accttaatca gaatttttcg
     8581 ctaaaaaatg acgttggcca cgtcatccgg aaagcacaaa atcacgtcga tttttaatga
     8641 acggttaaac cgtctttttg tcacggtgtg tcactggttg tcactctccg tcacgattga
     8701 ggtggtggtc attctgcccg accgcgcatc tttgcgctat cgggaatatc aaacagttac
     8761 ctcgccgact tcctcaaatt gaggtttccg aaaatatcag agcgggttag cgattaagct
     8821 gacgagtaat taaactgcga aaatttcgta gtttctgaat atattccggc ctgctggtta
     8881 acagtcctca gatgtgaggg atgtagccag cgcaattttg cgctttgctt taatctcagt
     8941 gagttaccgc cgtaaccgct ccggcttctt ccagtggtac gtgattttct ccttctcccg
     9001 atacagttca acgcggcgat tgtaggccag catttccaga acgcggatcc gtatgtcgcg
     9061 catatccgca tcattaagct ggataccatc acggcgcatc acctcagcaa caacacgcgc
     9121 ataattttcg gctgtcacgc tgtccggctg cgtggtctgt tcgtcagcct gctggctgat
     9181 tccagagacg cggcggatta atcgcagtat ttcggcttct gtcatgctgc tgacctcatt
     9241 acaccccgct aaattcttcc agtttctggc ggtggctgtc gcttatatca aaagcaaaat
     9301 cctcatgctc tgcctggaat gtaccaaacg ccatcagcgc cgctacgctc gggtctatct
     9361 tgttcggtga ttttttcttg ttcggcttga tattggcgtt cgcgtcactc tgcatcacga
     9421 cgttactcat cgaccaggac aacaccggat cgccacgatg cacaatcacc ctgcggttaa
     9481 caaaaacttc gaacgatttc gccgccggac tgaatctgag gtaggtttgc gggaacggct
     9541 ccacctcaaa accagccccc tgtaattgcg tcctgagatg cgtggcgttc cacgtatcaa
     9601 agcccaccag cctgatatta aatttctccg catcctgcat gatgtcatct ctgatccggt
     9661 cgtaatcaat gcagtcacct ggcgttgtgc gtatccagcc cgctttagcc cactggcgat
     9721 agacagcgcg gtttttattg gcggggttct gtagctggaa ctccggcaga taatgacggg
     9781 aaatcagcat aatggtttta ccgaccggaa aggcatagca tacgctggaa atatcgctgg
     9841 ttgatgataa gtccagcccc gcgtaacact cctgaccgtg taaatcttcc tccgtgaacg
     9901 ttccggcaca ctcagcccat gcaccattac ccatccacgg ggtagtcccc tgacaccaga
     9961 tattaaatcg ctttgtcatc atctccaccc actgcgacgg aataccccgc gctttctgga
    10021 tggttgaggc cagtttttca cgatccacgg aaacatcaat attagggttc gcctttatcc
    10081 acatcgccgg atcatcaacc tcgttttcgt cgtccagttc gtaaatcagt acaaaaattg
    10141 aatcgttaac ctcttcgccg tccaggatct ggcagcaata atcatagtgc tgtttacagg
    10201 ctgaaacgac gttactgccc gatgtggtaa tggcaaataa cagcccttcg ggacgtgcgc
    10261 ccatccccag ctcaagcgcg gaataaacgc cgttatcggg gtgtaggtgg tattcgtcca
    10321 cgatggaaag actggggttt gtcccctcga tggttgccgc ttttgctgcc agcggcttta
    10381 acaggctgtt gcttttcggg tgtatcacct tatgcgcctg aatattcacc cgcctgcgta
    10441 acggtcggga taaaaggcac atctgacgcg cattatcaaa cacgatccgc gcctggtcac
    10501 ggctcaccgc tgcggtgtag atatcctgct gaccgttctc cataatcaga aaccagttag
    10561 ccagaatcgc ggcggtcgtg gatttcgcat ttttgcgcgg cacttcgata aaggcgctcg
    10621 tgtatttgcg ccgtccggtg gccttaacct taaagccgag gatgcacgca aaggcgaact
    10681 gctgccacgg ctccagctca atgggtctgc cacgcatcgg tccttttacg tgcgggcaca
    10741 ccctggaaaa ggcaataaac cgctccacaa cctcacgatc gaacgtgtaa agggggcttt
    10801 taaggtccga aaagtaccgt ttaacagcct gttttaaccg tttacaggcc ggaattttgc
    10861 ccgtttttac gtcttctgcg tacttattcc aggcggtcaa gctcgtcctc ttcttctgtt
    10921 tccggtggat ttttacggcg gcttatcggg tcaaaaccga gcaaggaggc catttttatc
    10981 atcactcttt cagcgtcgga ttttgcgctt aatgcggggt ttctgctctc gccgccctga
    11041 ctgttaacaa tgctgaaccc gcgcgccgca aggtctgcaa cggctttccg gtaaatggag
    11101 tagttgacgc aatacagttc cagattgctc cagtcggcgg gggtcaggtc tccccgttcc
    11161 gcaagctgcc gcgatttttc ccgccactgc ttcacggcga tatcatccag gtaggcgggg
    11221 tcgggtttgt cctgaaaacg ccccccaccc cgtcatacag cctcatcagc gattgcggaa
    11281 acattccata acctcgcggt cacggtcggt taatcgcttc gctgtgatgc gttctgcgcg
    11341 tcctgacgct ttatctttat gccctgtttc ctgtgtcttc catgcgtcac gctgccttat
    11401 aagtccacgg ataaggcggt tttgttcccg ctcattcatc agcgtcatac atccagttat
    11461 tgcggttagc agcccgttct tcctcttcac gaaatccacc tgcggcacgc ttgctttttg
    11521 ttgccggatc aagccatttc gttttctggt tatgacacgc ctggcacaat ggttgatggt
    11581 tccattcggg ccagaagaga acatcatcac cgccattaat cgggataatg tgatccacca
    11641 ccacggcggg cgtatatatc cccttctcaa ggcatcgcac gcataacggg tttttactca
    11701 gatacatggc gcggtatttg tcccactgtc tggaatatcc acgcgcgcgg cggtgtcctc
    11761 gtctggcatc ctctgcacgc catgccgccc gcctgtgctc ctcacacttg ccggatttaa
    11821 cgcgcctgtt acagcctggt tctgtgcatc gtcttaatgg ttgccacggc atcagtaccc
    11881 ccccacatca cggtaagccg tccagagtgc gcctatcgtc atgggtacgc gtgtttttgc
    11941 gttatccgcg acaatctggc gattttcata caggtgagcg ataaacatca tgcagccaat
    12001 ctttatggct ggcgtgaacg ccagcccgtc atcaaagcgc ctgcctatgt gtatctggca
    12061 cgcctcaagc gatgcggcaa tgtatccgct gattaactgg tcttcatcgt cgccatcgat
    12121 gcggcaatgg agtttcactt cttccagggt gataagttct tctgtcattt ttccgcgccc
    12181 tcacgacaaa gaatttcaag gcgtgtcctg gcggcatccg gcagcggctg cccgatgata
    12241 ttcagtacac gccccgccag cggccccgta ctgaccttta tccggctggt ggcgttgatg
    12301 tccttccggt accgtatcca gatccttacg gtaccaatcg ccagctctgc gcccgatgaa
    12361 atggcctcct tgctgctgac catgttcacg cttgcccaga gtgtgtgacc gtcctcccac
    12421 gtttcgagta tttcgccggt tatatccctg gtctgtttca gggtctgaat cgttatcctg
    12481 tcacgcagtc gccctatgtt cattccggtt tttctccctc gctgattttt acttcctgtt
    12541 tccatgcctg gctgaactca tcaccaccat cacgcggtga cagcccttca cgttcgcggg
    12601 cttcgttcgg acacataacg tcggatttta tgccgcgctc ataggtggca aagcgttcgc
    12661 caggtgtggc ccgtaacagg tccgcgctgt caaactccac ctgataccgg ataccaggta
    12721 caggcgaggc caccagcaag gcgtttttta tctgctgctc aaagttcgcc agccacgggc
    12781 gcatcgtcat ggtgagaaat gcgcggcttg cctcgctgaa gttgctgtag gtgctgttgc
    12841 tgtattcctg caaaaaaatc ggcgagacgt tgaacattcg ggcgatgtct tcaatggtga
    12901 agcgacggga ggccagccat tcggcatcct gattactcat gcccagctgc tgatagctca
    12961 tccccccttc aaggatgggc gtttttccgg cgtttctggc ccctttgtag cgttccagtg
    13021 cggctaatgc ctgtttgccc ttcacgccgt ccagccattc gcctgacgtg ataacccctg
    13081 ccgccatcat gccatcacgc atcacgctcg cgccgtggcg ttgttgggcc agcccaagcc
    13141 ccagcgattc gcggcagatg gttacaggtg agcgccccat aaaaccgtca tccgtggagt
    13201 aacgaaggtg gagaacctcc cacggtaaat agttgcggat gtttccggtg taggcgtcag
    13261 tgatgcagta gcgccagtta tgatcacctg tctgctccac gttcaccgac tgcggcgggt
    13321 aaggatgtaa agccgccgga aaaccatcac gcccccactg aataaccgca taagcattac
    13381 cacttaacag gcagtagcgg atcatcattc gcttaaactg gtagggtgtc tgccacgcgt
    13441 tcgggcgctc gttgaggatg tgatcgaccg gatgagaatc aagccattcg cgggcctcct
    13501 tccccttctc attgcgtacc aggtacaggt aacacggcat ggtagccacc gcgtcagaga
    13561 tgaccgtgac ggcgttcatg acggcgggga gtgattccgc tgtcccagat gatacgtact
    13621 cacccgcccc cgtgttcgat gtgccggcca gcgccataaa ttcatcaagc gtcatgctgc
    13681 gctgctcttt ttttcgtctg aaaggccaca tatcacaccc ccgctaaatc cgcccaccag
    13741 cggcgattat ccgcacgcgg cattttttcg gggtgctgct catacaggga acggcgggcc
    13801 agctccacgc cggaatcggg gtaagccggt accgatgtaa cggtaatttc gtacagttcc
    13861 gccaccagca cggtgcgcac gcatggatct gttgtggtat cccatacatc cttacgggag
    13921 cgaaagccaa agctcatccc cgagatgtcc ccacgcttaa ccagttcgat aacgtcgcgc
    13981 cctgtactgg tatccggtgg ggtcagttca aagcgtaacc ctgtttcgtc ctcttccagt
    14041 ttcagcgtgc cggaacgggt gcgccccagt aacatgctgt ggtcatgctc atacaggccg
    14101 cgaacgtcat tacccgccgc aagccattca gtaaacgccc cccgctggaa tttttcgtaa
    14161 aactcacccc ataacagttc tgaaagctta tcccagcgaa caacgtagcc cgtcagcgta
    14221 ccggctccgc tggtggtgat ttccgatgac cggatttcca tactcttcat aattttttca
    14281 cccataaaac actaaagggg ctttttagcc ccttctgtat gctgttaatc gtcgtcttcc
    14341 ggcagtgcca gaatcttgat cgcgttcgaa tccaccacgc caccgcctaa atatttttgc
    14401 gtgaaaattt tgatgaatcc tggttctgtc aggttgtccg gtctggtgcg aacacctgtt
    14461 tcgtgatcaa caatgtagta accgcgtttg aagtcaccca gggcaataac gttatcaggc
    14521 ataaactcca gatattcgac cggaaggccc agcaacgtat caggatcacc cgcctgtaaa
    14581 cggtcgcgcc agatataatc accgttcgcg ttcttcacct tctgaagttt tgccgccgtc
    14641 gtggagttaa ccactcagac cgcgtttttg cggtattttt tacgtaatgc aaatttcagg
    14701 tcgatcagcg ggtccgcaga tgtccacgcc agagattcgg aaggtttaat tacctgcaac
    14761 gtaccaaaat cacgctcttt gtcgttcttc tctgcacggg gtacggataa aaaacctttt
    14821 gcttttttgt caccgtcgcc cacaaccaga tcgctttctt cggtttccgt gaaggtgtcg
    14881 ccaatctcac ccgtcagcca tgaaaggatg tccacatcgg aaaaatccac gatttcctgt
    14941 gtggtgcgcg ggtacgcata gaccggatac agcttaatgc tcacctcgtt aatctgcggg
    15001 gtgctggtct gttcgcgtgc cttaccctct tcaccgtggt taacggtcgc accgccagcg
    15061 gaaacaagct gcttaaactc gttgctgctg attttcttca cggtacagat gcgacgcatg
    15121 gtggattcat ccgccagcat tcgcatgatt tcggtgttca gttcagggat aacggtataa
    15181 ccaccatcag cgggaacgcc tgtacttaat gcgcgggttt caccggtcag aatgtagtta
    15241 cgtagttctg cggggtcagt ggtctggctg cttttacctg gcttgctgcg ctcttcgtct
    15301 gcaatggctt caaggcggga aatgtcttta tcgagggatt cagctttagc gcgtaattcg
    15361 tcaaattttg cgccctcagc atcgttaaga ctgcggtttt ctttttcagc gttctccagc
    15421 atgtcgcgca tctgattttt aatggcggtt ttctgctggc gtaattcgat tattctctgc
    15481 ataaaaaaag tcctggggtt aagtaaggaa ctccaggacg cggcaaacac tcaaccgttt
    15541 ttcataagga aatcagtaat cgcaccgatc gttttcccgc ctggtaatga atattggcga
    15601 gcacattaac aggcgggaaa gtggccccag cgtcctggca ccacgggcga gaataatcat
    15661 gattcagttc gggtaaaata tgccgatccg ctcagtgaac aacgtggaac aaccacgaac
    15721 aaataattta caaaaaataa caaaaagccg gattgctccg gctgtatgtg ctgtatgggt
    15781 gctcagtctc tgatcccttt cagaaattct atcaatgcat ctgtctgttc aggatttact
    15841 gccagcattt caccggatag cgcgcaacga acaaaaccat gatgatcctt ttcaatcagc
    15901 gcccccgttt ccagaaatgc gcggtaatca gtgatattca tcgtctccat gttgtcagca
    15961 tggtatttat cacgctgttt aagtatctca tcaaatttca tcggcattgc gttttcctct
    16021 gttgtgtctg tttgtttcag atagtaacta tgccggaccg tgacgaaaag ccagtagtgc
    16081 tccataccgt ttcaaatgac gcaaaaaaag ccggattgct ccggctgcgt ggtgtggttc
    16141 tgatattcct actctgctaa tttgctcagc cccatgtctg cgtaagtgcg atttactgca
    16201 tttctcaggt ctgcgtagtt ctccgttggc ggctccggtg tgcccttgct gctctgtttt
    16261 accagcgggt cgcccaccag ctgcccgtaa gctgcaattt gtgctgtcat attgccacct
    16321 cagtgaagcg atccggcatt gttctttttc atctcttcca tcattttttc agtgagcatt
    16381 acgcactgaa accccatgtt tttgatgtct acgggtttca tgcctttgtc agtggatatc
    16441 acaggcagaa tatcctgata cacatcgcgg tcagggtaac taagcacaca aagctcccat
    16501 gatgccacgg gtgaggatcc ttcgtctgtt tcaacccacc agcctggtgt agctgcgctt
    16561 atttgcagaa tcgtgatccg tccgttgtgc ttgtaaaatt tttcaccaaa ttttggttcg
    16621 ctcatgattc cacctctccg gttttaacgt tgattgttgt tacctgttcc gcttcggcaa
    16681 tctcccgctc tgtcagcgtg gcaaaatttg ccgccgtcgt ggtcatgaat gcgcttatca
    16741 gttcgggatg tgctttcgcg tatccttccc ccgcgtggcg gtctatcgtt ctgattgcca
    16801 cctttaaagc gtgctctgtc atgtctaacg cgcgatattt cggttctgtt ctgtctctgc
    16861 gttttttgaa tgatttatta aatttccctg aagtgtgcat atttattttt accccctcgt
    16921 ttaaaaagtt ttgagttgtg cctccccttg tctaccttat ctaccttagt ggccctcatg
    16981 ccagtaatgg cgctgctttc agcggggtag agtgctttta tccactatct accccgtgtc
    17041 tacctccctg tctgattcag gtaaaatcag gtagagaggg tagatagtgg gtagacagta
    17101 aaaaaaggct atctacctaa cttaatgcac tgaattaaat gtatttttct ttactcaggt
    17161 agacagggta gacagcaatt acaaaaaatt ataaaaacgc gtcgcactcg tctgttgtta
    17221 ttgcgttagt ctgcgttact cccttaactt tccgcgtaat atattcatgt ccgtaaactt
    17281 tcgcggctgg cttcatagcc ttgccaaagt catttacgtt tagcggtttg ctcctgcctg
    17341 cgtacgccat aaacgccaga tagacgcggt aaaggctgtt tctggtcgtg tacttcactg
    17401 aatcgccacc gccgcccatc atcaggccgc gtgcttcctc cagaaaattc aggaactggc
    17461 aaaactcaat aaccggatcc gtctgttgct ttattgccag agcttcatca ccgtcacgct
    17521 gttccagtag taaagcccgt gctttctcag agtcggtaaa gttcgccagc aatcggcgga
    17581 taatgacagg gatttcagcc gcaatctttt ccggtagctc cctgtctttt tcggcctcac
    17641 taacgatatt gtcgaaacgg aaaatcacgc gacgacgtgc cacacctccg gcccgttcgg
    17701 tgaatatcat cgggttattg ttggtcgcca gcaccaccgc cctgattaca gccgtgaaac
    17761 gcttttcgta tttcgggtta atttccacgg ggtcaccgcc cgtgattttc ttgatgcccg
    17821 ttccttcgcc tgtatatttc ggctggtcag ccaggacgat aagacgactc ccgacaacct
    17881 gcgcacgtcc accagcatca tcaagcgatg tcatttcagc gcttaccgtg ttctgtttcc
    17941 ctgccagaag gctggctatg tgtgtgaatg tacttttacc gctcccgccg tctccggtgg
    18001 cctcaataaa catctgccag tcgtaccggt tcgccataat catgtacagc gcggcacata
    18061 tacgcatcat cttgcgctgg tcttttccgg ctgcgtgctc aagccattta tgaaagttta
    18121 gcgcgttatc gcggatgttc tcccctggtg ctggtggcgt gtactcaatg ccgttgtgcg
    18181 tggtgatcca gttctccggc gtgtgcgggg aaaattcccc cgttttcagg tcaagcgcac
    18241 cattggcgaa cggcagcaaa tcgccggacg gctcgcccat tggttcggca ataactttta
    18301 acgcttccac ggcgttattg attacgcgct tgctgaaagt ggccctgtgc tctgaataga
    18361 tcaccaccat ttcgcggcta agttccattg tgctgaccgg acaccatacc ccgccgcgcc
    18421 atacgtgaac gatttcactt tcctgatgca cacaaacgcc atcaaagcgc tcggcaagca
    18481 gctgcgcgcg ctcactgtct gccatttgtg ccagttgcgt atttttctgc tccggcatgg
    18541 taagccctgc ggcaatattt tcacgctcag cattcagata gcgccgccag ttttcacact
    18601 tctggacgtg cattccttcg gggtaaaaat ttgcgtcctg aatatctgcc gccgccagct
    18661 tctgaccaat ctttttggtc tccaccaaat ccagttctcc ggcctggtac agccttacgc
    18721 gtttttttcc atccggaaca attttcagtg catcaagttc ggcaagctga tttggcccaa
    18781 gccacacagg cggcacatta tcgccggatg cggggccatc ctgctcctgc cattgtttcg
    18841 cgtgcgacca ggcatcacta cccgcaaaga taattacctc tgtgtctttg tgttttattc
    18901 cgcgtggctg tttttttacg ttcggtgcca gtttcatttt ttacccctga atacgttaag
    18961 catctttttt atttcctgaa tattggcgcg tgctttctcc ctgctggttg gtttactgcg
    19021 gggcgctgcc tgtaccagcg tgaaatcacg ccggaattga taaacaggca tcacgcaatc
    19081 atattcgtaa ccttcacggc ggtaggtgat gcgccgttct gccacgcctt taatcgttac
    19141 catgccgccg tactggtcgc ggtaaatatc accgcgcgta aatttagggt gagtgttgcc
    19201 actggcagtt aagccagaat atttaagttt cattattttt attctccggt gtgttgttct
    19261 ttatatctgt cgtgcaatag atctatttct tgcagttcca ttattacagg ctcaagaagc
    19321 gttattaatg ccgtggcaat tcttgatttt tgtttgtcgc gttcattgtc gccaagtgtt
    19381 tcaagccata tgcgcaatat ttccagcatg ttttcactgt gagaaagtgc aagaaatgcg
    19441 cggtctattg tttcgtggta aatatcacgc atattaatcc ccgtccgtcg tttttcttaa
    19501 aataacctct gtcacgaaat cagcataatc ggcggcgata ttcagtacat caagcccgtt
    19561 gatttatatt ctctcgtgga aagtaagaaa aaagccgctc taataagttc tggcattgac
    19621 gaaagcgcat cagccgcatc atcaggaacg ccggaaaatt cctgtttcag ggaattaaaa
    19681 cgatcatcac gcataacccc ccccattttc acaatcggca acaagaatat ttttagcgtc
    19741 attcagtgac cgcgttgcga tgtatcggat acattccagg gcgaactgtg tccggtcttt
    19801 ctgttcttct ccctgcgcaa gctctgccgt gcattcaata tcaataagcg cgtgcatcag
    19861 cgtagtgagt gcggcggctg ctgcgttcgg tgtggtttta ttgtgcatag gtttccccct
    19921 gacgaatacg ggcggcgaat acagcaacac aaccggacgg gcaacggcta cgcgcttcgc
    19981 gttccgtcca agcggttacg tggatgattt gggattctcc ggcactcagt gccagaaaac
    20041 gccacacaaa ggccgtttgt gtgtgtacaa ggtgtggtat atgatttacg gcaaccataa
    20101 cggctcctag tttacgttgt tggttagaag ccccgttact gctcctacag tgcggggctt
    20161 tgctttttgt gttgattgtg tgtaatgtgt cactacacat aaatacatta cctgaggtgt
    20221 aattaacgtg tcaacacaca aaaacgaaag gcgtggtaat cctccgttcc agttccgttt
    20281 agatcctgac ttacgggaaa agatggaaaa ggcacaacaa caggacggcg atgattctct
    20341 tgctgcctgg ataaagcgaa taatccgcaa agaattacag caacgcggca tagaacccaa
    20401 aggataaaaa ctatcagcgc cgtggtgtga ggtactacgg cgcattgctt tacaggcgca
    20461 cacaatgacc aacgaagaat caaccgaaaa aacatcacct gcacgaaaaa gacgacgcag
    20521 aaagaaagag catgaatcag aacgattcgc gccgtgttct tttgcccttg agaagtacct
    20581 taaagaatac tcatcaaagg agagaacttc gcagttatgg caacgcactg aaccagactg
    20641 attgcattgc ccaccagcct gatagcggct atcatccccg tgcttatgtt tgggatcaca
    20701 cacacaaacg gcgcagcggg ttatctgttc agaaaggcgg ctccatttcg gggccgcttt
    20761 ttttatgcct gaaaaacccc aattttgtgg ttttccagtt tcaccagggc gaacgaatcc
    20821 ccgcccacgt tctggcgtat attcaatctt catggttata gctctgtgtt cagatgattg
    20881 atgtgtggcg gctgcgtgcc gccagcgtga ttaatgaact gccttgcagc tatccttcca
    20941 ggccagaacc tcggataaag accagccaac ggaacgaccg ccaagtttac gacgtgatgg
    21001 gaattgtccg gccttttcca ggcggtagcg gcatgagcgg ctaaggcctg ttagcttttc
    21061 gcattctttt tcacgtataa accgatcagt gcttaacact attgccccct ttcgtttctt
    21121 aaagagttat ttcgtgttct attgcgttgg gatgtgtctg attgtgtcag gatgattcag
    21181 agttggcaaa tgttgaggcc gtatggttta cagaaagagg aataatcagg ataaaatcct
    21241 ttaaattcat gttaatacaa aggcataaaa tattgtttca tgcctttttt ctcgctattt
    21301 aaagagtgat tcgctagtgt ataaaaaatc agtaacacat taaaaatcag ctacttataa
    21361 atctgtacac tttttcacct cttgttcgta gttgttccgc attgttgctc attgttgcac
    21421 gttgtatctg ttcgcatatc cagtatgcgc atactgaaaa aacacgaaaa aaattatttt
    21481 cttctggcta ctggtagcgt ggttacgttt tcatgtgttc ccgccagtat ccctaaccgc
    21541 tccgtccaca tatccagcgc attgcgttta gcatccagat aacgggaatg attataaact
    21601 cgctgcattc ctggcatctg gtggcctgta agctgctcca cgacgtgagg atccacgcct
    21661 aaatcgttca gcatcgttgt aaaggtgcgc cggatgtcat gtagtgacca gtgagggtga
    21721 tttagcctcc tgtgcgctaa tctgccgtac tgcgacacgc ttgtttcctg tttcacttcc
    21781 cccagcaata agcccgtgtg cctgttctgc tccaccagct gcgtgacgaa cggcagtatt
    21841 gcttccggta tgggccggaa tattgccacc ttcgttttgc tgtgttcttt cggcacggtc
    21901 cagagcattt cggtaaaatc ccactcgctg atctccgata acctcagttc taccgtccgg
    21961 catccgaaca caatcaggag gcggattaac gcgatgtagt agggggagaa tatttttttg
    22021 tccagtgcct gcaataattc gcccagttct ttggtgctta agacacgctc gcttatatcc
    22081 ggttttttcc caacgtccgc cacactcata tcatcaagaa cgttgctgat tgcatagcgc
    22141 cgcctccggc agaacttaag cgcctgtttg cacgtctgta gcaagaatcc ggcagtaaca
    22201 ggcgttcgct ttgccacctg gtcaaaacag gccagccagt gccgtagctc gcatttatcc
    22261 agcggcatag caccaatgtg ctgtattacg tgattattaa ggcgcttttt cagggcggca
    22321 taatccacgc ggttttcctt tgcgtacgac tcaagccagt aggtgagcgc atcgccaacc
    22381 gttaccggct ttaacgcttc ctgtacggtg taattaagct catgacgtgg atttttcccc
    22441 tctgccagcc atgcgcgaca ctgggcggct ttttccctgg ctgatttcag gctcagatca
    22501 ggataatttc ccagcttaat gcgttccggt ggtgcccccc ttcccgttcc ggccctgtaa
    22561 gtgaaatacc aggttaaaag gccactggtt gaatgcctga cgctcaggtt tccaccgtca
    22621 ttaagaaagg ctgttttttg ggcgggtgtg ccgttgattt tcctcagctg tgtatcgctc
    22681 agtttgttaa gtgctctgct cataattttg attccggtca tcacaattaa ctacaccatt
    22741 aactacaccg atcgttgcac aaagggctac aacgtgaaac aagctggaac aaggaaaatc
    22801 acaaatctgt ttataatcaa aaacataatg aacaatctga aacattatga aacggcaaaa
    22861 aacactaatt gataaaatat gtccatactt cacgcattac gttaagcatc cgttataatc
    22921 ggttgcagat accagcctgt ggatgcttaa catggaatac caactcactc ttaactggcc
    22981 cgattttctt gaacgtcact ggcagaaacg cccggtggtg ttaaaacgcg gctttaataa
    23041 ttttattgac ccgatctctc cagacgagtt ggcgggtctg gcgatggaaa gcgaagttga
    23101 cagtcgactg gtcagtcacc aggatggcaa atggcaggtc agccacggcc cgttcgaaag
    23161 ctacgatcat ctcggtgaaa ccaactggtc attactggta caggcagtaa accactggca
    23221 tgagccgacc gccgcgctga tgcgaccgtt ccgtgaacta ccggactggc gtattgatga
    23281 tctgatgatt tctttttctg tacctggcgg cggcgtcggc ccgcatctcg atcagtacga
    23341 cgtgtttatc attcagggta ccggacgtcg tcgctggcga gtgggcgaaa agctgcaaat
    23401 gaaacagcac tgcccacatc cggatctgtt acaggtcgat ccgttcgaag ccatcatcga
    23461 tgaagagctg gagcctggtg atattcttta tattccgcca ggattcccgc atgaaggcta
    23521 cgcgctggaa aatgcgatga actattccgt gggttttcgc gcgccaaata cgcgggaatt
    23581 aattagcgga tttgccgatt atgtgctgca acgtgaactg ggcggcaact actacagcga
    23641 tccggatgtt ccacctcgtg ctcatcctgc ggatgttctg ccgcaagaga tggataaact
    23701 gcgtgagatg atgctcgaat tgatcaacca gccggaacac tttaagcaat ggtttggcga
    23761 gtttatatcc cagtcacgtc atgaactgga tatcgcgccg ccggaaccgc cttatcagcc
    23821 agatgaaatc tacgatgcgc tgaaacaagg tgatgtgctg gtgcgcctgg gtggtctgcg
    23881 cgtattgcgc attggcgacg acgtgtatgc caatggtgag aagatcgatt ccccgcaccg
    23941 tccggcactg gatgcactcg ccagcaacat tgcgctgact gcggagaatt ttggcgatgc
    24001 gctggaagat ccgtcattcc tcgcgatgct cgcggcgctg gtcaatagcg ggtattggtt
    24061 cttcgaaggg taagtttgga tttaagccgg atgcagcatc acatgccgca tcccttttcg
    24121 cttacttccg ttgcgccgtt aactcggcaa tacggacgat cacctgcacc gctttttcca
    24181 taccttccag aatcacaaac tcatgcttac catgatagtt gtaaccgcca gtgaacaggt
    24241 tcgggcaagg taatcccata aacgacaact gcgcgccgtc ggtaccgccg cggatcggtt
    24301 tcagttccgg ttcaatatcg cagtcacgca tcgcctgctg ggcgatatcg agaatatgcg
    24361 gatgctcaac cactttctcg cgcatattgt agtaactgtc ttcaatcacc aattcaatgt
    24421 agcaatcagg atgtaaccct ttgcccactt ttttggcgat ctccatcatt ttacgtttac
    24481 gcgcttcaaa ctgtttacgg tcgaaatcac ggatgatgta gtgcatatcg gcccgttcaa
    24541 cggtgccttt catgctcgcc aggtgataga aaccttcata gccttctgtc atttccgggc
    24601 tttcatccgc cggaacttcc gcatgaatac gtgccgccag cgacagcgca tttaccatca
    24661 ctcctttcgc cgtgcccgga tgaacattgt taccgacaat ttttatattg accgatgcgg
    24721 cgttgaagtt ttcaaactcc agttcgccta cgccaccacc gtcaacagtg taagcccagc
    24781 gggcatcgaa ggcatcaaca tcaaaatgtt tcgccccttt gcccacttct tcatccgggg
    24841 taaaggcgac gcgaatatca ccatgcggaa tgtttttctg ttgcaatacc gccagcgcgg
    24901 tcatgatttc tgcaatacct gctttgtcat cagcacctaa caaggtttta ccatcggtgg
    24961 taatcaacgt ctgacccagt agctgatgca gcaccgggaa cataaccggt gataaaactt
    25021 catcgccgat acccagcgca atatcgccac cgcgatagtt ttcaacaatt tgcggattaa
    25081 catttttgcc gctgcaatcc ggtgaggtat ccacatgaga aataaagcca atcgccggga
    25141 tatcgccagg gacgttagcc ggtaacgtcg ccatcaaagt gcccttctca cttaaggtca
    25201 cattgataag ccccatttct tcgagctgct ctttcagcag atgcaataac ttccattggc
    25261 cttccgtgct gggaacctgt ctcacccctg cttttgattg ggtatccaga gacacgtagt
    25321 tcaaaaatcg ctcaagtagt ttatccatgt agtcaccctc actttttgtg acaacattat
    25381 taagaagcaa gaaaagacaa atattgcgtc aggtcacttt tacccctgca agcgggaata
    25441 tttatcagca ttaccttaat gaatataaag ctaagccagt aattcacaac aaagattggc
    25501 gattcaagcg gtttgccgta gaattaccgc cctcattagg agtcaccaag gtggttaacc
    25561 acaaaccccg catcggtaag ccatccgttg cgtttacatg ggacagagta aaaaattgaa
    25621 taaacaaccg agttcgcttt caccgctggt gcaattggcg ggaattcgca aatgctttga
    25681 tggtaaagag gtcattcccc agctggatct gactatcaac aatggcgagt tcctcacgct
    25741 gcttggccct tctggctgcg gtaaaacaac cgttcttcgc ctgattgcag gtctggaaac
    25801 tgttgattcc ggacgcatca tgctggataa cgaggacatc acccacgttc cggcggaaaa
    25861 ccgctatgtg aacactgttt tccaaagcta tgcacttttc ccccacatga ccgtgttcga
    25921 aaatgtggcc tttgggttgc gcatgcaaaa aacccccgct gctgaaatta cgccccgcgt
    25981 gatggaagcc ctgcggatgg tgcagctgga aaccttcgct caacgtaaac cgcatcagct
    26041 ctctggtggt caacagcaac gcgtcgccat tgctcgagcg gtggttaaca agcctcgtct
    26101 gttgttgctg gatgagtcgc tctcagcgct ggattacaaa ctgcgtaagc aaatgcagaa
    26161 cgagctgaaa gcgttacagc gtaagcttgg cattacattc gttttcgtga ctcacgacca
    26221 ggaagaagca ctcaccatgt cggacaggat tgtggtgatg cgcgatggtc gcattgagca
    26281 agacggcacg ccgcgtgaaa tctacgaaga gccgaaaaac ctgtttgttg ccgggttcat
    26341 tggcgaaatt aatatgttta acgccacggt gatcgagcga ctcgacgagc agcgcgtacg
    26401 cgccaacgtt gaaggccgcg aatgtaatat ctacgttaac ttcgccgttg aaccggggca
    26461 aaaactgcat gttctgctgc gcccggaaga cttacgtgtt gaagagatta acgacgacaa
    26521 ccacgctgaa gggctgattg gttacgttcg tgagcgtaac tacaaaggca tgacgctgga
    26581 gtcggttgtt gaactggaaa atggcaagat ggtgatggtc agcgaattct tcaatgaaga
    26641 cgatcctgac tttgaccact ctctcgacca aaaaatggcc attaattggg tagaaagctg
    26701 ggaggtcgta ctggctgatg aagaacacaa gtaagttcca gaatgtagtg attgtcacta
    26761 ttgtcggttg gcttgtgttg tttgtctttc tgcccaacct gatgatcatt ggcaccagct
    26821 ttttgacccg cgacgacgcc agtttcgtca aaatggtctt tacactggat aactacacgc
    26881 gtctgctcga tccgctctat tttgaagtgc tattgcactc gctgaatatg gcgctgatcg
    26941 ccaccctcgc ctgcctggtg ctgggctatc catttgcctg gtttctggcg aagctgccac
    27001 acaaggtgcg tccgctgctg ctgtttctgc tgattgttcc gttctggacc aactcattga
    27061 ttcgtatcta cgggctgaaa attttcctca gcaccaaagg ctatctcaac gagtttttgc
    27121 tctggctggg cgttatcgac acaccaatcc gtatcatgtt cacgcccagt gcggtgatta
    27181 tcggtctggt ttacattctg ctgccgttta tggtgatgcc gctgtactcc agtatcgaaa
    27241 aactggataa gccgttgctt gaggcggcgc gcgatctcgg tgccagcaag ttacagactt
    27301 ttatccgcat cattattcca ctgacgatgc cggggattat tgccggatgt ctgctggtga
    27361 tgctgccagc gatgggcctg ttctatgtgt ccgacctgat gggcggtgcg aaaaacctgc
    27421 tgatcggtaa cgtcatcaag gtacagttcc tcaatattcg tgactggccg tttggtgcag
    27481 ccaccagcat tacgctgact atcgtaatgg gcctgatgtt gctggtttac tggcgcgctt
    27541 ctcgtttgct gaataagaag gtggaactcg aatgatcggt cgactgcttc gcggcggttt
    27601 tatgaccgct atctacgcgt acctgtatat cccaatcatt attttgattg tgaactcctt
    27661 taacagctcg cgctttggca tcaactggca gggttttacc accaaatggt atagcctgct
    27721 gatgaacaac gacagcctgt tacaggcagc gcagcattca ctaacaatgg cggtgttttc
    27781 ggcgacgttt gctacgctta tcggttcact gacggcagtt gcgctgtacc gttatcgctt
    27841 tcgcggtaag ccgttcgtta gcggaatgct gtttgtggta atgatgtcac cagatatcgt
    27901 gatggcaatt tcgctgctgg tgctgtttat gctgctgggt attcagcttg gcttctggtc
    27961 gctgctgttc tcgcatatca ccttctgcct gccatttgtg gtggtgacgg tgtattcgcg
    28021 cctgaaaggt tttgacgtgc ggatgctgga agcggcgaaa gatctcggtg ccagcgaatt
    28081 taccattctg cgaaaaatca ttctgccact ggcaatgcca gcggtggcgg cgggctgggt
    28141 gttaagcttt accctgtcga tggacgatgt ggtagtttct tcattcgtca ccggaccaag
    28201 ttatgaaatt ctgccgttaa aaatttattc gatggtcaaa gtcggcgtat cgccggaagt
    28261 taacgcgctg gcaaccatat tactggtgct gtcgctggtg atggttattg ccagccagct
    28321 tattgctcgt gataaaacga aaggtaacac aggggacgtt aaatgaaaaa atggtcacgc
    28381 cacctgctcg cggcgggtgc tctggcactg ggcatgagcg ccgctcacgc cgatgacaac
    28441 aacacgctgt atttctacaa ctggaccgag tacgtgccgc caggactgct tgaacagttc
    28501 accaaagaaa ccggtattaa ggttatctat tcgacttacg agtcgaacga aaccatgtac
    28561 gccaagctga aaacgtacaa agacggtgcc tatgacctgg tggttccttc aacctattac
    28621 gtcgataaaa tgcgtaaaga ggggatgatc cagaagatcg acaagtcgaa gttaacgaac
    28681 ttcagcaatc tcgatccaga catgctcaac aagccttttg acccgaataa cgactactcc
    28741 attccgtata tctggggtgc gacggcgatt ggtgttaacg gtgatgcggt ggatccgaaa
    28801 tctgtcacca gctgggccga tctgtggaag ccagagtaca aaggcagcct gctgttgacc
    28861 gacgatgccc gtgaagtgtt ccagatggcg ctgcgtaagc tgggctactc cggtaacacc
    28921 accgatccga aagagattga agctgcatat aacgagctga aaaaactgat gccaaacgtc
    28981 gcagcgttta actccgataa cccggctaac ccgtacatgg aaggcgaagt taacctcggc
    29041 atgatctgga acggttctgc tttcgttgca cgccaggcgg gtacgcctat tgacgtggtg
    29101 tggccgaaag aaggcggcat attctggatg gacagcctgg cgatcccggc aaatgccaaa
    29161 aacaaagaag gcgcgctgaa attgatcaac ttcctgctgc gcccggatgt ggcaaaacag
    29221 gttgctgaaa ctatcggtta tccaacgcca aaccttgcgg cgcgtaagct gttaagtcca
    29281 gaagtggcaa acgataagac gctctacccg gatgctgaaa ccattaaaaa tggcgaatgg
    29341 cagaatgacg ttggcgcagc cagcagcatt tatgaagagt attatcagaa gctgaaagca
    29401 ggacgttaat tcagcaagcg ggcggtggta ttaccgcccg ttttttagtt agcaaggatg
    29461 tggctaatga gtcaggacag taaagtttta tcccgctctt tcgtcggtat tgtggtccgc
    29521 ttgtaggcct gataagcgta gcgcatcagg caatgcttcc tgctttataa tccctccagc
    29581 aacttttcaa caaattctgg caccacctgg cttgccgggc cgtaatattt ctcggcaaat
    29641 tcattaccaa cctgactcgg ttcaagattc agttcaacgg tgtgagcacc atgtagtttg
    29701 gcttcgtgta caaacccagc cgccggatag acatgcccgg aagtaccaat ggcaataaag
    29761 acatccgcca tcgacaacgc catataaatt tcatccatgc cgagtggcat ttcgccaaac
    29821 caaacgacgt gcgggcgcaa gggtgccggg aactggcaac aatggcattt atcttctggc
    29881 gtaacgtctc cggtccagtc gagaacctga ccactttgcg aacaacgaac tttcagcaat
    29941 tccccatgca tatgaatgac attggtatta cctgcgcgtt catgcaggtt gtctatattc
    30001 tgcgttacca gcaaaaagcg atcgccgaga gcgtcttgca gtttagccag cgcaagatgc
    30061 gcggcgttag gctgaatttc tggctgctgc agctgtcgac gacgggcgtt ataaaacgct
    30121 tgcaccagtt caggatcgcg atcgaaacct tccggcgttg ccacatcttc aacccgatgt
    30181 tcttcccaca ggccatctgc ggcgcgaaag gtacgaatac ctgattccgc agaaattcct
    30241 gcccctgtca gtacgagtac tcttggtttt tccattgctt ccggcaccac tttatctctg
    30301 aaaaaaatac gctgacgcaa acgctcgcgc aggcgacgtt tatttttacg aaaacgactt
    30361 aaccgatgac cccgacgcga cagcatagca acctctgttg tttaatcggt tagatgtagg
    30421 aaggccgcac cacgcattcc tcccgcatca ccgtggcgcg cgcgttcaat gcgcggaaca
    30481 cgagctacag gtaagagatg acaaggcaac ctgtccgcca gttgcgttgt gattgccggg
    30541 aaattcgata agccaccacc aatgacgacc aggtcagggt caacaatggt caggatattt
    30601 cccagagaaa ccgctaataa atccagataa cgctcaacgt gcgcccttgc ctgctcatcg
    30661 ccttgatcat aaagcgcaat aatttcggga gcctgcaacg gttgatgata atagtgttga
    30721 tacagccacg caaaaccgcg accagacaga taattttcaa tgcagccatg ctgaccacag
    30781 ccgcagcggc gtaacgggaa atccaacccc atcatggtta acgcatcaac cggcagacgc
    30841 atatggccaa actcgccggt aatgtagctt ttaccagtaa tcggtttgcc gttgaaaacc
    30901 agcccgccgc caacgccggt gccgagaatt aaccccatca ccagtggata ttgcgtaaat
    30961 tcatcatccc aggcttctga aagggcaaaa cagttggcat cgttatcaag gcgtacatcg
    31021 cgatcaagac gtgcgctcag gtcggcacgc agcggtttgc cgctggcagc ggggacatta
    31081 gcggcataca gcttaccatc ttctgtttcc ggcattcccg gaataccgat gccgacagaa
    31141 cctttacagc caaaacgttg gtcagcttca gctaccagct cacacactgc atctaaaaat
    31201 gcgtcatagc tgtcacgcgg tgtcggcacc cgcttttccc actgcaactg ccgaccgcta
    31261 tcaaacacgc caagcgcaat ttttgttcca ccaatatcaa acccgtaata cattgccgct
    31321 ccttgtttta atgtactgcc tttactggcc gctaaggact cgcgcagggt caatattact
    31381 ggcgcgccgc gccggatacc aacttgccaa aagactcagc aacaatgctg tgaccagtac
    31441 gtagaagacg tccagccagt gcaattccga tggcaggaag tcaataaaat aaatatcgct
    31501 ggagaggaac tgatgaccga tcagcttttc aatccactca ataatcgggg taagttgcag
    31561 tgaaacaact acgccgataa ccaccccaca caggctgccg aacagccctg ccagcaatcc
    31621 ataccagaca aagatggcgc gaattaaacc atctttcgcc cccagcgttc ttaatactgc
    31681 gatatcgcca ctcttgtctt tcaccgccat cactaaggtg gagacgatgt tgaaacaggc
    31741 cacgccaatc accagtacca tcgccagata cataatggcg cggatcatct ggatatcgcg
    31801 atacatatag ccgtaagtac caatccaact tttaatataa acatagctgt tggtcacttc
    31861 acccgcatcg cgtaccagct tattggcgtt gaaaacatcc gtcattttaa gggcaatacc
    31921 tgacacgctg gaacccatat caagatattg ttgggcatcc gccagcggga tcatggcaaa
    31981 actgtgatcg agttgaccac tcaactgcaa aataccggca atgtgcaaac gcacacgttt
    32041 tggctgcatc agtttatgct cgggattcga gttggggatc atgatcgaca cccaatcgcc
    32101 ctgcttcact ttcagcgcat ccgccacgcc tttgccgatg ataatttgct gttcgcccgc
    32161 tttaaaattg cgccacgcat ccccctgaac aaacgagggt aatgcgctca gacgctgttc
    32221 ctgttgtggg ttaacgccct tcacctggat tgcgcgcaga ttcgctccac tttccaccag
    32281 accggtgaaa ttgatatacg gcgcagcggc ggcaataccc ggcactttct gcacgttatc
    32341 cagtgcttcc tgccagttag tccatggctg atttaccgct tcgatttcgc catgcggcac
    32401 caccgccaga atgcggttat tcagttcgcg ttcaaagccg ttcatcgcgc ttaagccgac
    32461 gatcaatacc gccacgccga gggcaatgcc aatggtagaa atgacagaga ttagcgacac
    32521 catgccgccg cgtcgccgtc cgcgactaaa acgcaggcca atcaataacg ataaaggcat
    32581 cgccattact ccgcccccat caggctcagt tccgccgtca gacgtccatc acgcatttcc
    32641 agttggcggc tcatacgttt cgccagttgc aggtcgtgag tgaccaccag gaaagcggtg
    32701 ccctgcaagc gattcaactc cccaagcaac tggaagatgc tgtcggcgtt acgcgcatcg
    32761 aggttaccgg taggttcatc cgccagtacc agacgcggat tattgaccag cgcacgggca
    32821 atcgctacac gctggcgttc gccgccagaa agttcagatg ggcggtgatt cgcacgatgc
    32881 tccagcccca ccgcttttaa catctccagt gcacagctgt tgatttcagc gggctttttc
    32941 ttgccaatca gcagtggcat agccacgttt tccagggcag taaaatccgg cagcaggtgg
    33001 tgaaactgat aaataaagcc cagcttctgg ttgcgcagtt ctgctttcgc cgctgaagac
    33061 agtttgctca tcggctgacc gttaaagatc acatcgccgg aggttggcgt atccagcccg
    33121 cctaacaggt gcagcaaggt acttttaccg gaaccagagc taccgacaat tgccatcatt
    33181 tcaccttcgc ccacgctgaa actgacattg agcagcacat cggtttgcac actgccttcc
    33241 tgatagcgtt tgcacaggtt gtcgcattgc aacaggatct tattcataac gtaaagcctc
    33301 agcgggttgt gtggcggcag cgcgccatga agggtaaagc gtagacagca gcgcgatagc
    33361 catcgccacc agcgcaataa caatgacctg taaaggttcg atagccaccg gcagcgccgc
    33421 gccatcaagc aggacgccga ttatcggcat cagattattt aactggctgg caagcagtgc
    33481 gccaagcgcc gctccgagga ttgcaccgat aatcccggcg ctagcccctt gcaccataaa
    33541 gaccatcatg atttgtcgcg gagttaagcc ttgcgtttgc aggatcgcga cttcgccctg
    33601 cttctccatc accatcagcc ccagcgaggt aataatgtta aacgccgcaa cagcgacaat
    33661 caggctcagc agtaagccca tcatattttt ttccatgcgt acggcctgga acagctcgcc
    33721 tttacggtca cgccagtcct gccatttgct gccttcaggc agtttttgct gacttaaaga
    33781 gtcaactctc agcggctcat ccagccacaa acgccagccg gtaatattgg ctgccggata
    33841 acgcatcaga cgcgaggcat cctcaatatt caccagcatt tcatagccat cgacttcact
    33901 gttggcggcg aaagtgccaa tcacattgaa caggcgctga cttggaatac gccccatcgg
    33961 cgtgaactgg ctggcagatg gcaccatcac gcggatttga tcaccgcgat tgacgcccaa
    34021 ctgtgaggca agttgttcgc cgaggatgac attatatttc cccggctcga ggtcagtttg
    34081 tttcacattg accagatacg gcgttagtgg atctttttgc gccgggtcga taccgagcat
    34141 caccccgacc gccacgctgc gcgcgctttg cagtaccaca tcaccggtag taataggtgc
    34201 gacgcgatta acgccgtcca gtttgactgc cgtttccggg agttgctgcg ggttaagaga
    34261 gccatgctca gaagagagaa ttgcctgtgg catcaggcca aggatgttgt tttgcagctc
    34321 gcgctcaaag ccgttcatca ctgacaatac tgtgaccagc gccatcaccc cgagggtaat
    34381 gccgatggta gaaagccagg agacgaaacg accgaagcga tccactgcac gcccacgcat
    34441 gtaacgcagg ccaataaata gagcgacagg ttggtacatg aaatccgtct ggttgctgta
    34501 gcaaagacac gagtatataa gcgaaagcaa gatgggtaaa cgtctgaact gtaagtgatc
    34561 gtcagggcta atctgaaaag cccccgtaaa tcaatcggct aaattgccat attttatcat
    34621 caatcttgcc gcctgatctt aatctgaaaa atcatccaat acagacagat acccattaca
    34681 cctcgggtca taaccagcag gatcaaccgc atgtaaatta aaaaagagcg tgttgttctg
    34741 atgaaacaaa aagagctatg gattaaccag atcaaagggt tatgtatttg tctggtggtg
    34801 atttatcact cggtcattac cttttatccg catatgacca cttttcagca tccgttatcg
    34861 gaagtcctga gcaaatgctg gatctatttc aatctttacc ttgccccctt tcgtatgccg
    34921 gtttttttct ttatatccgg ctatttgatt cgccgctata tcgacagcgt accgtgggga
    34981 aattgtctcg ataaacggat ctggaacatc ttctgggtgc tggcgctttg gggcgtggtg
    35041 cagtggctgg cgttaagtgc gctgaaccag tggctggcac ctgagcgcga tttaagcaat
    35101 gcctccaatg ccgcttatgc cgattccacc ggtgagttcc tgcacgggat gatcaccgcc
    35161 agcaccagct tgtggtatct gtatgcgtta attgtctatt tcgtggtatg taaaattttt
    35221 agccgtctgg cgctgccact attcgccttg tttgtactgc tgagtgtggc ggttaatttc
    35281 gttcccacgc cgtggtgggg aatgaacagt gtgatacgca atttgcctta ttacagcctt
    35341 ggcgcatggt ttggcgcaac aataatgacc tgtgttaaag aggtgccgtt gcgccgccat
    35401 ctgctgatgg cttctttgct taccgttctg gcggtcggtg cctggttgtt tactatctcg
    35461 ctgctgttgt cgctggtatc gattgtggtg atcatgaagc tgttttatca gtacgagcaa
    35521 cgattcggta tgcgctccac cagcctgctg aatgtgattg gctccaacac cattgctatc
    35581 tacaccaccc atcgcattct ggttgaaata ttcagcttaa ctctgcttgc gcaaatgaac
    35641 gcagcacgct ggtcgccgca agtcgaactg acactcctgc tggtttatcc ctttgtcagt
    35701 ttgttcatct gtactgttgc gggcttgctg gtaagaaaac tttcacagcg tgcattcagc
    35761 gatctgttgt tctccccgcc ttcactgccc gctgccgtca gttactctcg ttaaaccatc
    35821 accccagccg gggcgattca atttgctaaa ccatgtcgat tacggataat gaacagcatt
    35881 gcttatcaga gatatgcccc catatgttga ggcatatcct aacgagaatc tgacaaccgt
    35941 tatgcctgaa caatatcgtt atacgctgcc cgtcaaagcg ggtgagcagc gtctgctggg
    36001 cgagttaacc ggcgcagcct gtgcaacgct ggtagcggaa attgccgaac gtcacgccgg
    36061 tccggtggta ctcattgcac cagatatgca aaatgctctg cgtttgcatg atgaaatcag
    36121 ccagttcacc gatcagatgg tgatgaatct ggcggactgg gaaactcttc cctacgacag
    36181 tttttcgcct catcaggaca ttatctcctc gcgcctttcc accctttacc agctaccgac
    36241 gatgcagcgt ggcatactga ttgttccggt gaatacgctt atgcagcgcg tttgcccaca
    36301 cagttttctc cacggtcatg cgctggtgat gaaaaaaggt cagcgcctgt cacgagatgc
    36361 attacgaacc caactggaca gcgccggtta tcgccatgtt gaccaggtga tggagcacgg
    36421 cgaatacgcc acgcgcggcg cgttgctgga tctcttcccg atggggagtg agctgcctta
    36481 tcgtcttgat ttctttgatg atgaaatcga cagcctgcgg gtgtttgacg tcgacagcca
    36541 gcgcacgctg gaggaagtag aagcgatcaa tctgctgccc gcgcacgaat ttccgaccga
    36601 taaagcggca attgaactgt tccgcagcca gtggcgcgat accttcgaag tgaagcgcga
    36661 tccggaacat atttaccagc aagtgagtaa aggcacatta cctgctggga tcgagtactg
    36721 gcagccattg ttcttcagcg aaccactgcc accgctgttc agttatttcc ctgccaatac
    36781 cttgctcgtg aatactggcg atctggaaac cagtgccgaa cgtttccagg ctgacacgct
    36841 ggcgcgtttt gagaatcgcg gcgtcgatcc gatgcgcccg ctgttgccac cacaatcgct
    36901 ctggctgcgg gtggacgagc tcttctcaga gctgaaaaac tggccccgag tgcagctaaa
    36961 aactgaacat ttaccgacaa aagccgcgaa tgccaattta ggtttccaga aactgccaga
    37021 cctggccgtt caggcgcaac aaaaagcgcc gctggatgcg ctgcgtaagt tcctcgagac
    37081 tttcgacggt ccggtggtgt tctcggtaga aagtgaaggt cgccgtgaag cgctgggtga
    37141 actgctcgca cgaattaaaa ttgctccgca acgcattatg cgtcttgatg aagccagcga
    37201 ccgtgggcgt tatctgatga ttggcgctgc cgaacatggt tttgtcgata aggcgcgtaa
    37261 tctggcgctg atctgcgaaa gcgatctgct cggtgaacgt gttgcccgcc gtcgtcagga
    37321 ttctcgccgc accatcaacc ccgatacact gatccgtaac cttgcggagc tgcatattgg
    37381 tcagccggtg gtccatctgg agcacggcgt cggtcgctat gccggaatga ccacgctgga
    37441 agctggcggc attactggcg agtacctgat gctcacctat gccaacgacg ccaaactgta
    37501 tgttccggtg tcgtcactgc atctgattag ccgttacgca ggtggcgcgg aagaaaacgc
    37561 cccgctgcat aaacttggcg gcgatgcgtg gtcacgcgcg cggcagaaag cggcggaaaa
    37621 agtgcgtgat gtggcggcgg aattgctgga tatctacgcg caacgcgccg ccaaagaggg
    37681 cttcgcgttt aaacacgatc gtgagcagta tcagttattc tgcgacagct tcccgtttga
    37741 aaccacgccg gatcaggcgc aggccattaa tgcggtactt agcgacatgt gtcagccgct
    37801 ggcaatggat cgtctggtgt gcggcgatgt tggctttggt aaaacagaag tggcgatgcg
    37861 cgccgctttc ctggcagtag ataaccacaa gcaggtagcg gtgttggtgc ctaccaccct
    37921 tcttgcgcag cagcattacg acaacttccg cgaccgtttc gccaactggc cggtacgtat
    37981 cgaaatgatc tcccgtttcc gcagcgccaa agagcagacg caaatccttg cggaagtggc
    38041 ggaagggaaa atcgatattc tgatcggtac gcacaaactg ctgcaaagtg acgtcaagtt
    38101 taaagattta ggcctgctga ttgtcgatga agaacaccgc ttcggggtgc gtcataaaga
    38161 gcgcattaaa gcgatgcgcg cgaacgtgga tattctgacg cttactgcaa cgccgatccc
    38221 acgtacgctg aatatggcaa tgagcggaat gcgtgacctg tcgattatcg ccacgccgcc
    38281 cgcccgtcgt ctggcagtta aaacctttgt ccgtgagtat gacagcctgg tggtccggga
    38341 ggcgatcctg cgtgaaattt tgcgcggagg acaggtttat tatctctaca atgatgtgga
    38401 aaacattcag aaagccgccg aacggctggc agaactggtg cctgaagcac ggattgccat
    38461 cggtcacggg cagatgcgcg agcgcgaact ggaacgggtg atgaatgatt tccatcatca
    38521 acgtttcaac gtgctggttt gtaccaccat tatcgaaacc gggatcgaca tcccgacagc
    38581 caacaccatt atcattgaac gtgcggatca cttcggtctg gcgcagctgc accagttacg
    38641 cggtcgcgtc ggacgttcac atcatcaggc atatgcatgg ctgctgacgc cgcatccaaa
    38701 agcgatgact accgatgcac aaaaacgtct tgaagcgatt gcctcgctgg aagatctcgg
    38761 ggcaggtttt gcgctggcaa cgcacgatct ggagattcgc ggcgcgggtg aactgcttgg
    38821 cgaagaacaa agcggctcaa tggaaaccat cggtttctcg ctgtatatgg agttgctgga
    38881 aaacgccgtc gatgcactga aagccggacg cgagccgtcg ctggaagatc tcaccagcca
    38941 gcaaacagaa gtcgagctgc ggatgccgtc gctattgcca gatgatttca tccctgacgt
    39001 gaacacgcgt ctgtcgttct acaaacgtat tgccagcgcc aaaacggaaa acgaactgga
    39061 agagatcaaa gtcgagctta tcgatcgctt cggcctgctg ccagatccgg cgcgtaccct
    39121 gctggatatt gcccgtctgc gccagcaagc gcagaaactg gggatcagga agctggaagg
    39181 taatgagaaa ggcggggtga tcgaatttgc cgagaagaat cacgttaatc cggcctggtt
    39241 gattggtttg ctgcaaaaac agccgcagca ttaccgcctt gatggtccga cgcgcctgaa
    39301 atttattcag gatttgagtg agcggaaaac gcgtatcgaa tgggtacgcc agtttatgcg
    39361 tgaactggaa gagaacgcga tcgcttaatg taaatcgggg gcgtttctgc gcccccatct
    39421 gcaacattta caaattcttt gcactttcct gcactatccg acactgtcac catccataat
    39481 tcaggcttat ctgtttatta caataacctt atatttatta tgtatttttg gtgatgatca
    39541 aaacgcgttt ttctcgctgg ctaacgtttt ttacgttcgc cgctgccgtg gcgctggcgc
    39601 taccggcaaa agccaacacc tggccgctgc cgccagcgag cagtcgtctg gttggcgaaa
    39661 acaaatttca tgtggtggaa aatgacggtg gttctctgga agccatcgcc aaaaaatata
    39721 acgtcggctt tctcgctctg ttacaagcta accccggcgt tgatccttac gtaccgcgcg
    39781 cgggcagcgt gttaacgatc ccgttgcaaa ccctacttcc agacgcgccg cgcgaaggca
    39841 ttgtgatcaa cattgcggag ctgcgtctct attactaccc gccgggtaaa aattcggtaa
    39901 ccgtgtatcc aataggtatt ggtcagttag gtggtgacac gctgacaccg acaatggtga
    39961 ccaccgtttc agacaaacgt gcaaacccaa cctggacgcc aacggcaaac atccgcgccc
    40021 gttataaagc acagggaatt gagttgcctg cggtagtgcc ggctggaccg gataacccaa
    40081 tgggccatca tgcgattcgt ctggcggcct atggcggcgt ttatttgctt cacggtacga
    40141 acgccgattt cggcattggc atgcgggtaa gttctggctg tattcgtctg cgggatgacg
    40201 atatcaaaac actctttagc caggtcaccc caggcaccaa agtgaatatc atcaacactc
    40261 cgataaaagt ctctgccgaa ccaaacggtg cgcgtctggt tgaagtacat cagccgctgt
    40321 cagagaagat tgatgacgat ccgcagctgc tgccaattac gctgaatagc gcaatgcaat
    40381 catttaaaga tgcagcacaa actgacgctg aagtgatgca acatgtgatg gatgtccgtt
    40441 ccgggatgcc ggtggatgtc cgccgtcatc aagtgagccc acaaactctg taaatcctgg
    40501 ataaaaaaaa tccccgcgac acggcatgtc gcggggaaaa aatcagcagt agcaggcatt
    40561 aatgagggtt aatgcttatt tataaattac tgctgttcca tggagggtat tcggaccggt
    40621 tacagaagta atacggaaag attttgcgcc catctcatcc gctttttgcg ccagctgctc
    40681 ttccagcgat cccagatttg tccccgcgtt agcactgata gtaccgactt tttgttggcc
    40741 ttctggcgtt gactgaactt cgacagccgc aaagttggca aatgacatgg agcttaaaat
    40801 cgccgcagcg atgaggtttt tacgtttttc ataatagtgg ccttatgcag atgaatgacg
    40861 tcgcaagaaa ttacttaacg atcgataact aaatgataga tgtgatctgg atcacataca
    40921 agatattttt tataacaatc attaattaat ttaaaaatct ttaaatttca gtcaaataga
    40981 aagcaattat ttttatggga tctggactgg tgaacggagg tgattatttt tataataacc
    41041 tttcattcac agtcaccagg tacaacggca tcatggcaac tgactcaaca caatgtgtaa
    41101 aaaaaagccg tggccgccca aaagtgttcg acagggatgc cgcgcttgat aaggccatga
    41161 aattgttctg gcaacacggt tatgaagcga cttctcttgc ggacctcgtc gaagcaaccg
    41221 gagccaaagc gcccacgcta tacgcggaat ttaccaacaa agaggggtta tttcgtgccg
    41281 ttctcgaccg ctatatcgat cgttttgccg ctaagcatga agcacagctg ttttgtgaag
    41341 agaaaagcgt ggagtctgcg ctggctgact attttgctgc catcgccaac tgctttacca
    41401 gcaaagacac cccggcaggc tgcttcatga tcaacaactg caccaccctc tccccagatt
    41461 caggagatat agccaatacg ttgaaatcac gccatgcgat gcaagagcgc actttgcagc
    41521 agtttttatg tcaacgacaa gcgcgcgggg aaatcccgac ccactgtgac gtgacacatc
    41581 tggcagaatt ccttaattgt attattcagg ggatgtcgat cagcgcacgc gaaggtgcat
    41641 cgctggaaaa actgatgcag attgccggaa cgactttgcg tttatggccc gaactggtga
    41701 aataagtcgc aaaaaaggcc cctcagcctg agcgaatgag gggccaaatt gcagagtaca
    41761 gccttgttat actttgttat tcaaaatcag ttgcccgtta ctatctagcg ggatctgcgt
    41821 acccggatcg cggtccatgc ggattttgcc ctgctgatcg ccaatcttat aggtcacatc
    41881 ataaccgagc attttttctg acttgtcata caccgtttta caacgctgtt gcgtagtcgt
    41941 gtaagtatcg ctttcctgga gagagccctg gatctggtta ccggcatatc caccacccag
    42001 cgcccccaca acagtggcga catctttacc gcgaccacca ccaaactgat gccctatcac
    42061 gccaccagca acagcgccga gcaccgaccc ggtaatgcga ttttcatcct gcaccggtcg
    42121 acgatgggtc actgtgacgt tgcgacactc ctgacgcggt gttttaaccg tttccttgat
    42181 tggggttgca gaaacaacct gagcgtattg cgggccccgt tcaaacacgt tcagactggc
    42241 cactgccgct acgcccaacg cagctgcgac accaatcccg atacccgcca acattgattt
    42301 attcacggga acacctcctt ctttgcagtt atgccaatca tgcaagcgaa ggagaaaagc
    42361 agctatcaga cagaagatga aaagcgcggg aatgactgga aatacttaag gattcggagg
    42421 agtctgaagg aatgcctgat gcgacgctta acgcgtctta tcaggcctac cgctcgtacc
    42481 atttgtccag ggcggataag gcgtttatac cacatccgcc agtgtacgtc gattaatgca
    42541 acttcaaacg cggacggata acgcggttaa tactccccac cagcatcatt aatccggttt
    42601 taaagtaacc atgcagcgca atctgatgca ttcggtatag cgagatatat acaaagcgcg
    42661 caattcgtcc ttcaatcatc attgagccgc gcgtcaggtt acccatcagg ctaccaacgg
    42721 tggagaagtt cgacagcgat accagcgaac catgatcttt atactgataa tttttcagcg
    42781 gcttaccgtt catctgcgcc agaatgttgt tcattgcgca agtcgccatc tggtgtgcag
    42841 cctgagcacg cggcggaaca aagccccctt ccggacgcgg gcatgacgcg cagtcgccaa
    42901 tagcgtaaat gtctggatcg cgagtggttt gcagcgtcgg ttccaccacc agctggttga
    42961 tacggttagt ttcaagacca ccgatatctt tcaggaagtc tggcgctttg atcccggctg
    43021 cccataccat cagatcagcc tcaatatatt cgccatcttt agtgtgcagg ccgccttcat
    43081 cagcactggt gaccatggtt tgcgtcagca cgcgaacgcc aagtttcgtt agctcgttgt
    43141 gggccgcagc agagatacgt ggcggtaatg caggcaaaat acgttctccc gcttctacca
    43201 gcgttacgtt cagggcttcg ttggtcaggc ctttgtaacc gtagctgtgc agttgcttga
    43261 tcgcgttgtg caattcagcg gagagttcta cacccgtcgc gccgccgccg acaatcgcaa
    43321 tgttcacttt gccatttgcg cccaggttgg cagagtattt caggaacaga ttcagcatct
    43381 cctggtggaa gcgacgcgcc tggtgcgggt tatcgaggaa aatgcagttc tctttgacgc
    43441 ctggcgtatt gaaatcgtta gaggtgctac ccagcgccat gaccagggtg tcataggcga
    43501 ttttacgttc cggaaccaac agttcacctt tctcgtcgcg tagttctgca atagtgattg
    43561 ttttcgcttc acgatcaata tcaatgacgg aacccaactg gaactggaaa ccatgattgc
    43621 gcgcatgggc cagatagctc aacgcatcga cgccttcatc aagcgagcca gtcgccactt
    43681 cgtgcagcag tggtttccac aggtggctgt ggttacgatc gaccagcgta attttggctt
    43741 ttttcttacg tcccagctta tgccccagct gtgttgccat ttccagccca ccagcaccgc
    43801 cgccgacaat cacaatcttt ttcaatggcg tagtcaacgt gcccccctta aatttattaa
    43861 ccaattgtta attaatggtt attaacatag ctcttaatta acaacaggtt acagcaatga
    43921 aaatgttctt cgctattgag aataacatga atggtgcatt ggtcatacca aaattgatgt
    43981 taatcaagtt ttgttgctta aaagataggc aactttaccg ggggagatca aaaaatagag
    44041 tttattgttt attatttgag caaattcgga agggtaatat agggtgaagc gtatatcgag
    44101 ggcgtttggt cgtcctgata agatgcgcag catcgcatca ggcattgatg ctcgactgtc
    44161 ggatgcggcg tggacgcctt atccgactta cagaattcgg agcgcatcag gtaccggcaa
    44221 ggttacctga tgcgacgagg gcatttaccc gagggttttg aacgctttaa tgcgctgtaa
    44281 atgcggggag atattcttga atttgtgcgt ctgctcttcg tcccagacaa tctcgtaata
    44341 atgatgcaac tcttcagatg tccgctggct gttaagtgct tcatcattac gcgacaaaat
    44401 aaccagacaa cgatcgcgat tcttctcgcg gaagttggtc acacacttag tggcaatatc
    44461 ggcatactct tccgggcgat caatcttgcc ttccatgttc tcataaggga acaaattagg
    44521 gttgaagatc acctggcgga tatcgcaaag aaaaccaatc cgttccgccc agtatccgcc
    44581 caggccaacg ccgcaaatta gcggacgctc gtcaacgttc agttgcaaca ttttgtccac
    44641 ttctttaagc agatgctgca tatcatgttt cggatgccgc gtactgtagc ttatcaagcg
    44701 tacatccggg tcaataaact gcaattgtaa gactttctcg tggttacccg gactgttaga
    44761 gtcaaaaccg tgtaaataga taatcatcgc ttcctcacat aagccagggt tagtgaccta
    44821 ctttctcttc ctgccagcgt tcatgtaact gattcagacg ggtgctgatc gctttccagc
    44881 gagccgagtc catcagttcc tgtcgcgaaa atgaaccttt atgatacaaa cgtgtaacac
    44941 gttctgcctt gatcggtgac agattatcta acacgctgac ggccccttta cgattattgc
    45001 agaccaggat catatcgcaa cccgcatcca gtgatgcctg cccgcgttcg gcataactgc
    45061 ccataatcgc ggcaccttcc atcgataaat cgtcagagaa aatcacgccg tcaaacccca
    45121 gttcctgacg caaaacggtt ttcagccagt agggagaacc gctcgccgga cgcggatcaa
    45181 catcactgta gatcacatgc gcaggcataa tggcgtcgag tttattttcg cggattaagg
    45241 aactgaagac cgacatatct ttagcgcgaa tctccgcttg tggacgtgga tcgcacggtg
    45301 tttctttgtg tgagtctgcc gttactgcac cgtgtcctgg gaagtgtttc ccggtcgttt
    45361 tcattccggc ttcatgcata ccatcaataa accggctggc aattgccagg gctttttgtg
    45421 gatcggcatg ataagaacgc tcgccaatcg ccgcgctgat atgcccgaca tccagcactg
    45481 gcgcaaagct gatatcaata tccatagcga tcatttcgct ggccatcagc caacccgcct
    45541 cttgcgccag tttgccgccc tcttccattc ccaacagcgc agcaaaggat tgtgctgccg
    45601 gtaagcgggt aaaaccttcg cgaaaacgct gcacgcgtcc accttcctga tctaccgcca
    45661 ccaccagatg attgcgcgat gctgcgcgga tctggcgcac cagttcacgt aactgggcag
    45721 gatcatgata gttacgcgta aagagaatca gccctcccac cagcggatgc gccagtattt
    45781 cacgctcttc cgcgtccagt tcgtaacctt cgacatccaa cattactgga cccacactgc
    45841 tctccttatt gttttattaa tagctgccgc caggtgtcat cggccagcct gataaattgt
    45901 tgatcgccgg tttgtcgcca gcggtactca aaccaccctc ctttgagcat cagcagccag
    45961 ggaaaccatc gcctgacctg acgccataat tgcgccggat aaatcttcgc gcgagtggca
    46021 tagtcattga ccaattgccg gtgctgttca gtattttcca cccacaccgc cgccagttcc
    46081 agcgcgatat caccatctcc agcatactcc cagtcgatga gttttaaccc tgatgcgcta
    46141 tgcactaaat ttccggcgtg gacatccata tgcaatggac ttaagcgtaa aggtcgtggt
    46201 tcccgcgctt tgcgcagacg ttttaacatt cgcagccaac ccacagtccg ccgcgccgga
    46261 tcgctttgct gccagtacag ttccagtaag ggcaacagcg ttattcgcca gccaaaacgt
    46321 ggttgttgat gtagataata cagcaagcct gccagttcgt tggtatccgg caaatacgtt
    46381 tttaccgcac cgggcagata gtcgactacc atccagtcac ggagatataa atgcggcttc
    46441 ggtgcaatgc ttgcgggtag ttgtgataaa gcccgatact ggcgcaagaa cgcggactgc
    46501 ggcgcatcag gatcgtgcgg ctgacgcaca acaaaacgct ggccctgatg ttcaatgaga
    46561 aaactaccgc cactaagccc actattaaac gtcgtgacgg gatgaaactg cgggaaaaag
    46621 cgcgacagca attcgtcgcg cgtgatggga ttattgctgc gaaacggcac ctttacctga
    46681 ccagataatt tcgcccgtct gcaccagcat cagctgcatt tgtagggtcg gagcgttaac
    46741 gttgccagag gcgcaggagt acagcacgta atgagcgccg acattgcggg caatgcctat
    46801 ggctttacta cgggtgccta aactgtcctg cggcgacaaa cctaactgtt gcttagccat
    46861 cgacagttgc tgggcggaaa ccagggtaaa tttcccgtta ttagccagcg catttcgcag
    46921 cgtttcggtc gcttctgcgg cattcagcga accgttagta cggttgttaa cgctatcaac
    46981 cagcaggacg ctacccgcag tcaccccgtc agccccaagc atcttactga ccatcggctg
    47041 cattgcgcca ttccagtcat aatggcgaat atgcggcgca ggcggtgccg ttcgatcttc
    47101 gtgctcaatt gggcctggct gctgcgggat cgtcggcacc gagggcactg tggggacagg
    47161 ctgttgtggc tcggctggtt gttccggcgc tggtttcact tcttctaccg gtgcaggttc
    47221 acgttgcccc acacacccgg cgagaaacat cgccagcgcg gtaatcaagg cgtagcgact
    47281 catttttgtc atcaagattc acccctttac aaatatagat aaagtctgac tttgtgcgcc
    47341 cccagaaaat tggcgctgcc gtacagcgtt accgccgaat gtgcgggaat ggtaacgctg
    47401 cgtggccttt ccagaggatg catctccagc cctctggcgt cataccagta aaaacgataa
    47461 tgaacggtaa cgggttcttg cctttcgtta taaatcgttg aggatgctga aggttgaata
    47521 tcagacgtcg aaaggacggg cttttctgca ctgatgcccg cagccaataa agatgactcc
    47581 atcaccagcg attgctcatc attcaccgga atttccggat gtgaacgaca gcccaccagc
    47641 agtaacaaca ccagagacac cagcccaaag catccttttc tcatcgttac agacctttat
    47701 gcgccagcat tggtcccagc ggacggccac ccaacaagtg catatggatg tggtaaacct
    47761 cttgtccgcc atggcggttg gtattcatga tcagacgata gccatcttcg gcaatacctt
    47821 cttgctcagc aatttttgcc gctacggtga tcatgcgtcc cagcgcctgc tcatgctcag
    47881 ctgacacgtc gttcacagtc ggaatgagaa tattcggaat gatcagaata tgcgttggcg
    47941 cttgcggcga aatatcgcga aacgccgtta ccagatcatc ctggtagacg atatcggagg
    48001 ggatctcacg acgaataatt ttgctgaata tagtttcttc tgccacgaca ttttcctttt
    48061 tcaaatagcc atgcgttgcg ccatgctacg ccgcgccaca ctgcgagtat agagtatggt
    48121 cgagttaaac ctgctctttc aatcttaact cacgatttct taagcaaaaa atcgttttgc
    48181 tgccgtatat atcgccatta ttcccatttc tgcctgcgaa tgcgctgaaa agacattctc
    48241 tgtttggttt acacgacaaa tatgaatgcg tatatttctc atttgcattt gcatttgcat
    48301 ttgcagcaaa attattcgca cactaaatat aaccgctcat caccctcctc cggcatgagc
    48361 ctgacgacaa cataaaccaa gagatttcaa atgctttcaa cacaatttaa cagggataat
    48421 caatatcaag tcatcaccaa accgtcacta cttgccggtt gcatagcact ggcactatta
    48481 ccttctgccg cttttgctgc accagccact gaagaaacgg tgattgttga gggttcagcc
    48541 acagctccag atgatggcga aaatgattac agcgtaacgt ctacctctgc gggtaccaaa
    48601 atgcagatga ctcaacatga tattcctcag tcggtcacta ttgttagcca gcagcggatg
    48661 gaagatcagc agttacaaac gctgggcgaa gtgatggaaa acacgctggg gatcagcaaa
    48721 agtcaggcgg attccgatcg tgctctttat tattcccgcg gattccagat cgataactat
    48781 atggttgatg gtatccccac ctattttgaa tcgcgctgga atctgggcga cgcactttct
    48841 gatatggcac tgtttgaacg cgtagaagta gtgcgtggcg cgacaggact catgaccggg
    48901 acgggtaatc catctgcggc aattaatatg gttcgaaaac acgcgacctg tcgtgaattt
    48961 aaaggcgatg tctcggcgga atacggtagc tggaacaaag aacggtatgt ggcggattta
    49021 caaagcccac tcaccgaaga cggtaaaatc cgcgcgcgaa ttgtcggcgg ctaccagaat
    49081 aacgactcat ggctggaccg ctacaatagt gaaaagacct tcttctcggg cattgtcgat
    49141 gctgatttag gcgatcttac gacgctttca gccggttacg aatatcagcg cattgatgtt
    49201 aatagcccaa cctggggcgg tttaccgcgc tggaatactg atggcagcag caacagttac
    49261 gatcgcgcac gcagtaccgc acctgactgg gcgtacaacg ataaagagat caacaaggtc
    49321 tttatgaccc tgaagcagcg gtttgctgat acctggcaag cgacactgaa tgccacccac
    49381 tctgaagtcg aatttgacag caaaatgatg tatgtcgatg cctatgtaaa caaagcggat
    49441 ggtatgctgg ttgggccata cagtaattat ggacctggct ttgatcatgt cggcggcacc
    49501 ggttggaaca gtggcaaacg taaagttgat gcgctggatt tgttcgctga cggtagttat
    49561 gaattgtttg gtcgtcagca caatctaatg tttggtggca gttacagcaa acaaaacaat
    49621 cgttcagttc atgggccaac atcttcccgg atgaaattgg cagtttctac aactttaatg
    49681 gcaatttccc acaagccgac tggtcatcac agagcctggc gcaggacgat accacacata
    49741 tgaaatcgtt atatgctgcc actcgtgtca cccttgccga tccgctgcat ctgatcctcg
    49801 gcgcacgtta taccaactgg cgggttgata cgctgactta cagcatggag aaaaaccaca
    49861 ccacgcctta cgctggtctg gtgtttgaca tcaatgacaa ctggtcgacc tacgccagct
    49921 atacctctat tttccagccg caaaatgatc gtgacagttc aggcaaatat ctggctccaa
    49981 tcaccggtaa caactacgag ctgggtctga aatcggactg gatgaatagc cgtctgacca
    50041 ccacgttagc catcttccgt attgagcagg ataatgtcgc tcagtccacc ggtacaccta
    50101 tccccggcag caacggcgaa accgcctata aagcggtgga tgggacagtc agtaaagggg
    50161 tggaatttga actcaacggc gcaattaccg acaactggca gctgacattt ggcgcaacgc
    50221 gctatcttgc agaggataac gaaggaaacg ccgttaatcc taatctgcca cgcaccacgg
    50281 ttaaaatgtt caccagctat cggttgcctg tcatgccaga gttgacagtc ggcggtggtg
    50341 ttaactggca aaatcgcgtg tataccgaca ccgtgacacc gtatggcacc ttccgcgccg
    50401 agcaaggtag ctacgcgctg gtggatctct tcacccgcta ccaggtgacg aaaaacttct
    50461 cgttacaggg gaacgtcaat aacctgttcg acaaaaccta cgataccaac atggaaggtt
    50521 ctatcgtcta cggcgcaccg cgtaatttca gcattaccgg cacgtatcaa ttctgaataa
    50581 cacctgtaaa aaaggcagcc atctggctgc cttagtctcc ccaacgtctt acggattagt
    50641 ggttacggat gtactcatcc atctcggttt tcaggttatc ggatttagta ccgaaaatag
    50701 cctgaacacc agaaccagca accactacgc ccgctgcgcc cagtttcttc aggccagcct
    50761 gatccacttt agacacatca gcaacgctga cgcgcagacg ggtaatacat gcgtcgaggt
    50821 tagtaatgtt ttctttacca ccaaatgcag caaccagagc cggtgccatt tcgctggtac
    50881 ctgttgcttt tgcatcttca gtcgcgtctt cacgacccgg cgttttcaga tccagtgctt
    50941 taatcagcac gcggaagatg gtgtagtaaa caatcgcata accgataccg acgatcggta
    51001 acagccacag tttgctgctg ttaccagaca gaacgatgaa gtcgatcaga ccgtgcgaga
    51061 acgacgtacc gtcacgcatc cccagaagaa tacagattgg gaatgccagg cctgccagaa
    51121 tcgcgtggat gatgtacagg atcggcgcaa cgaacatgaa gaagaactcg atcggctcgg
    51181 tgataccggt caggaacgag gtcagcgccg cggagatcat aataccgccc actttcgcgc
    51241 ggttttctgg tttagcagag tgccagatag caattgcggc agctggcaga ccgtacattt
    51301 tgaacaggaa gccaccagac agtttacccg cagtcgggtc acccgccata taacgcggaa
    51361 tgtcgccgtg gaaaacctga cctgctgcgt tggtgtattc accaatctgc atctggaaag
    51421 gtacgttcca gatgtggtgc agaccaaacg gtaccaggca acgttcgatg aaaccgtaaa
    51481 tgccaaacgc aactaccggg ttctggtaag cagcccactg agagaaggtc tggattgcag
    51541 aaccaatcgg cggccaaatg aaggacagca caacgccagt aaagatggca gccaggccag
    51601 aaatgatcgg cacaaagcgt ttaccggcaa agaagccaag atactcaggc agcttaatac
    51661 ggtagaaacg gttaaacatg tacgctgcaa tcgcaccgga gataatccct ccgagtacgc
    51721 cagtatccgc caagtgttta gaggcgattt cttcagcagg taaatgcagt accagtggcg
    51781 caaccacggc catggtttta accatgatgc cataggcaac aactgcggcc agcgcggata
    51841 cgccatcgtt attggtaaag ccgagggcga caccgatcgc aaaaatcagt ggcatgtttg
    51901 caaagacgga accgcctgct tctgccataa catgcgatac aacggcgggc agccagctga
    51961 aattcgcgga accgacgccc agcagaatac ctgcgatagg tagtacggat accggcagca
    52021 tcagcgattt accgaccttt tgcaggttag caaatgcatt cttaaacata attgagagtg
    52081 ctcctgagta tgggtgcttt ttttacgttc tcacgcgtgg caagggggga gagcctcgcc
    52141 gtgtacaggg catctaagcg ccctttattt attacacaga gtaaaataat tcagtgccaa
    52201 tttgtttgac ggctatcacg tttcaacttc agatcattgg agaagttgca cattttgaca
    52261 aaacatatgt cacaatattt caaacgcggt tattcaccgc ccaattttcg gcggtgaact
    52321 ttactcgttt tagccattaa ttacgagctt taaaaaaact cattcaacgg atggattgaa
    52381 ggcgggaagc gtcgatgtga aacagacggg cgaagttatc ggtggttacc tgcgccagtt
    52441 cttcaacggc aacacctttc aacacagcca tgtattctgc aacgtcacga accatcgcag
    52501 gttgattctc ttttcctcga tgcggtaccg gcgcaaggta aggtgagtca gtttccacca
    52561 gtaatcgatc cagggggaca taacgcgcag catcgcgcag ttgctccgca ttacggaagg
    52621 tcacaatgcc ggaaaaggag atgtaaaatc cgagatccag taatttaccc gccgtttctc
    52681 tgtcctctgt aaaacagtgt agtacgccac cgcaatccgt cactttttct tcgcgcagaa
    52741 ttgccagcgt atcggcacgg gcgtcacggg tatggacgat aaccggcttg ttcagttcac
    52801 gaccaatctg gatatgatgg atgaaggact cttgctgacg tactttagtt tccggtgtgt
    52861 aataataatc cagcccggtt tcacccagcg ctacaacacc ctcttctgcc gccagacggc
    52921 gtaaatcttc tacatcgtag ggatcattct ggttgagtgg atgcacgcca caagaaaata
    52981 cgacgttgtc acgttcgcct accagatccc gcatatgtaa ataacccggt aatgttgtgg
    53041 cgactgccag acaaaatttc acatcgcgtg cggcggcttt cgccagaacg tcatccacgt
    53101 ccttatgcaa agattcataa tccagaccat cgagatggca gtgtgagtcg actaaaaaca
    53161 tgatgtctct cttaaagatg aggaaccggt aacacaacgc ccggttgcag gtaatgctca
    53221 atacgcagta aaagatcggt gatgagaagc tcgcggttga tgcctgtgac agacattaac
    53281 tgttcacgaa tgtggcaaac atcccccagt atagcctgca ggcacgaggg agaaagatgg
    53341 tttgccagtt cggcgaccag gcccggcaca tcaacattgg tcacctgcgc agcgccatga
    53401 tggcgtttta gcgcatccat cagcaacgtt gccagccagt gtaaacgcgc cggagcttgt
    53461 tcatgattaa gggccgccag cagcgaatac cagtcgcccg atggcacgct atatgccaac
    53521 gcctgacaca atgtttcacg agcctgccag ttatctccct gaaacaacgc cagtgccgcg
    53581 ccaggcgaac cggcgcttaa gcgcaatgcg gcaagtaatg catcctgtga cattgtcact
    53641 tcgcgtgaaa gccaagtcac ggcgtactgt tctggaggcg gcgcaaggta atgtaaccga
    53701 caacgactac gtaatgttgc cagtaaacgt tcaggctcgc gggtagccag gaaaaaccag
    53761 gtttctgctg gtggctcttc aagcgttttc agcaatgcgt tagccgcggc gtcggtcagt
    53821 aaggcagcat cggttaccca gacaactttc gcaccaccta ggcgtgcgtg ctcattcagc
    53881 ttttcggtga cctcacgtac cgcatcaatg cccagcgtat ttttcccttt ttcgggagcc
    53941 agggtgtagt aatcgggatg cgtgccagcc tgcatcaact gacatccacg acagtgaccg
    54001 caacttttgt ggccctgcgg ttgttggcag agtaaataac ggctcagggc gtagattaaa
    54061 gcatcatcgc ccatgcccgg taacgcctga atgagtagcg catggtgacc tcttccggcc
    54121 tgatagctgg ctaccagttt ttcgaaatca ggtcgtaacc atggatacca tctcatgcgt
    54181 ccaactcctt cacccagtgg gtcacggtag tgcggattgc atccatcacg gcctccagcg
    54241 gctgggtggc atcaatggta tgaatacttt tatcttgtgc agccagttcc agatagcggg
    54301 cgcgggtgcg attaaagaaa tcgaaagact cttgctcaat acgatccagc tcgccgcgcg
    54361 cacgcgcgcg ttttaagcca acttccgggg taacatcgag atagagcgtt aagtctgggc
    54421 gaaaatcccc gagaacagca tcacgcagtg ttgccagcat atgttggtca ataccacgtc
    54481 ctccgccctg atacgcctga gtggaaagat cgtggcgatc gccaatcacc caggtgccgt
    54541 tagccagcgc aggtttgatg accgtttcta ccagttgaac gcgcgcggca taaaacatca
    54601 gaacttcggc tttatcggta atgacttcat cgcctaccga tttgatatcc agcaccaggc
    54661 ttcttaactt ttcggcaagt tgcgtaccgc caggttcccg agtgaaaacc atgtcgcgga
    54721 tacccagttg ctcgagcgtc tcaaccacca cattacgcgc ggtagttttg cctgcgcctt
    54781 ccagcccctc aatgacgata tacttactgc gcattttttt ccttaagcac tttcagataa
    54841 tcctgcacag acttgttatg actggcaaga ttggtattaa acgtgtgacc acctttacca
    54901 tcggccacaa aatagagata cggcgttttt gccggatgcg cagcagcctt cagcgaatcc
    54961 gcccccggcg tagctatcgc acccggcggc aaaccggtaa tggtataggt gttatacgct
    55021 gtcggcgttt ccaggtctgc acgagaaagt ttgccattat aacgctctcc catcccgtaa
    55081 atcacggttg ggtcggtctg caggcgcata ccaatgcgta aacggttgat aaataccgag
    55141 gcaacctgat cgcgttcact ggcaacggcg gtttcttttt cgataattga tgccatcgtc
    55201 accagctgat ttttatcttt ataaggcaga ccgtccgcac gcccttccca ggcgctatcg
    55261 accgctttca ccattttctt gtgaactcgc ttgagtaacg cgacatcggt ggtattggcg
    55321 gtatacatca aggtgtctgg ccagaaccaa ccttcaatcc actccgggtt ttccagttca
    55381 agtgcctgcg ctacggtggc gtacttatcg tcactcagcg tgtgcttgat atacggagcc
    55441 tcacgcaatt gcttgaggta gtcgctcaga cgcatccctt ctaccagtcg cagcgggaac
    55501 tgtgcttctt taccgctttc caacaatttc agcatctcgc gcacggtcat ctgcggtgta
    55561 aagcggtaag tcccggcttt aaagtgagaa agatccggtt cgatacgcag cagccattga
    55621 aaaacccgtg ggcgattgat gatcttatca gcataaagct gttcaccgag cgccagacgt
    55681 ccggtccctg gcttcagggt aaatatcgtc tcttctttga taagcaattt gctgtcggca
    55741 agatggcgaa ccttccagac gcccacacca gcggcgatac ccagtaccac cagcaataac
    55801 aagataatca ataacacttt tttcatgact aattcgggcg ctcacaaagt ggggctaaat
    55861 attcatataa cgttgctgac gaaaaggtga catcgccaca ggcacgtacg ggcatcactg
    55921 gcattaacgc attacaaata accatctcat ctgcctgcaa cgcctcctcc agcgaggctt
    55981 gcacttcgac aagctgataa gaggattgtg ccagtaaacg gatacagaat tgtcgcataa
    56041 tgccgttaac acctgcctga tccagtcgcg gcgtataaac tacattgccc ttccgccaga
    56101 acaaattagc cgcacagcat tccgtaaccc acccttcgct gtcaaggacc agcgcctcat
    56161 cagcgtttgt ctgctcaaga tgagagcgaa tcaatacttg ctcaagccga ttaagatgtt
    56221 taatacctgc aagatgagga ttgcgcccca gccgcaccgg gcttagcgcc aacgtcatcc
    56281 cttcgttacg caaacggtcg taatgtgcag gataagccgt aacggagaga atccgcgttg
    56341 ctggtccgct gttcaatgtg ctgtaccctc gcccgccact accgcgactg atcacgactt
    56401 tcagcacacc attttgctgt tctgctgcca gcgttttcat ctcttgttca agctgaggcc
    56461 agaagtcaca ggaaatcatc aaccgctgac aagcatcctg tagtcgcagg atatgcgccg
    56521 ataacaaact gactttaccg tcgataactc tggcggtggt aaaacaacca tcaccaaact
    56581 gcgttgcccg atcgcttact gccagcgatt cctgcttata accgttaatt aagaacatac
    56641 cggctcctta tcagagaatt gttagtgtgg cagcatgttc actacggaac aagtcggaat
    56701 aaaagctaag aaaaaaggcc cgcaagcgga ccttttatat gggtggaaaa tgacaactta
    56761 gatcttttta aagatcaaag aaccattagt gccaccgaag ccgaaggagt tacacagcgt
    56821 gtattccatt ccgctgacct gacgtgcttc atgcggtacg aaatccagat cgcaaccttc
    56881 atccgggtta tccaggttga tggttggcgg aacagcctga tcgcgcagcg ccaggatgga
    56941 gtagatagat tctactgcac ccgccgcacc taacaggtga ccggtcatag atttcgtgga
    57001 gcttaccaac acacggcttg cagcttcacc gaagatggtt ttcaccgcct gcgcttcagc
    57061 tttatcgcca gccggcgtag aagtaccgtg cgcgttaacg tagccaatct gactcgcttc
    57121 aatgcctgca tcacgcagag catttgccat tgccagagct gcgcctgcgc cattttctgg
    57181 cggtgacgtc atatgataag catcgctgct cataccaaag ccgacgagtt cagcgtaaat
    57241 tttcgcaccg cgttttttcg cgtgttcgta ctcttcaagt accagcatac cggcaccatc
    57301 gcccagtacg aagccatcac gctctttatc ccacgggcgg ctcgccgctt gcgggttatc
    57361 attgcgggta gataatgcac gtgccgcgcc aaaaccacca acgcccagcg gcgtactggc
    57421 tttctctgcg ccacctgcaa ccatcacgtc agcatcgcca tacgcgataa tacgcgcagc
    57481 atggccaatg ttgtgcacgc cggaagtaca ggcggtcgcg atagagatgc ttgggccacg
    57541 caggccatac atgatagtca gatgacctgc caccatgttc acaatcgttg acggaacgaa
    57601 gaatgggctg atcttacgtg gaccaccgtt catcagagat gtgtggtttt cttcgatcag
    57661 tccgaggccg ccaatcccgg agccaattgc ggcaccaatg cgggttgcgt tctcttccgt
    57721 tatttcaagg ccagaatcct gcatggcctg aacgccagcg acaattccat attgaatgaa
    57781 ggcatccatc ttgcgctgtt ctttgcgcga gataatgtcc tcacagttaa aatcctttac
    57841 taagccagca aatttcgttg cataggcgct agtatcgaaa tggtcgatta ggctgatgcc
    57901 actctgaccg gcaagcagag ctttccaggt agactctacg gtattgccga caggagacaa
    57961 catgcccagt ccggtcacaa ctacacgacg cttagacacg tttgtcctcc aaggagggaa
    58021 aaatgattct agtgggacaa aaagataaaa ctcaggcggt cgaacgaccg cctggagatg
    58081 ttcacttacg cctggtggcc gttgatgtaa tcaatggcag cctgaacggt ggtgattttc
    58141 tcagcttctt cgtccggaat ctcagtatca aactcttctt ccagagccat taccagctca
    58201 acggtgtcaa gagaatccgc gcccaggtct tcaacgaaag aagcattgtt ggtaacttct
    58261 tcctgcttaa cgcccagctg ttcgccgata attttcttaa cgcgttcttc gatagtgctc
    58321 atactcttaa atttcctatc aaaactcgct ttcgcgatgg ttttcgtagt gtataaaatg
    58381 ttgaaaaatt tgcaactaaa tcccggcagg tcttaccacg attttacgtt attttgaggc
    58441 cttacccccc aataacgcaa atcattttgc actaattatt actcataacc acgcagactg
    58501 cgcgcaacat gagcattttg tgcaaatcgc ggtcagacca tgtacatccc gccgttcaca
    58561 tgcaaagttt cacccgtgat gtaagctgct tcgtcggatg ccaggaatgc aaccgcgttg
    58621 gcgatttcct gtgcgccgcc gaggcgaccc gcaggaacct gcgccaggat acccgcacgc
    58681 tggtcatcgc tcagcgcacg tgtcatgtcc gtttcaataa agcccggagc aacaacgttt
    58741 acagtaatac cgcgtgacgc aacttcgcgc gccagtgatt tactgaagcc gatcaagccc
    58801 gctttcgccg cagcgtagtt ggcctgaccg ccatttccca tggtaccaac cacagaaccg
    58861 atagtgataa tacgaccatg acgctttttc atcatagcgc gcattaccgc ttttgacaga
    58921 cggaaaacag atgaaaggtt agtttcgata atatcgttcc actcttcatc tttcattcgc
    58981 attaacaggt tatcacgagt gataccggca ttattgacca ggatatccac ttcaccaaat
    59041 tctgcgcgaa ttttttccag aacagattcg atagatgccg ggtcggtcac attcaacatc
    59101 agacctttgc cgttggcacc taaataatca ctgatcgcct gagcgccatt ttcactggtc
    59161 gcagtgccaa taactttcgc gccacgggct gcgagcgttt cagcaattgc gcggccaatt
    59221 ccgcggcttg caccggttac cagtgcgatt tttccttcaa aattcatgat tttcctcttt
    59281 taaagctcga gcgccgctgc catcgctgaa ggttcgttca gcgccgaggc ggtcagggtg
    59341 tcgacaatgc gtttcgtcag gccagtaagc accttgcccg ggccgacttc atagagatgt
    59401 tctacgcctt gcgctgccat gtactcaaca gacttcgtcc actgaaccgg gttatacaac
    59461 tgacgtacca gtgcgtcacg gatggcatca ccattggttt cgcatttcac atcaacgtta
    59521 ttcacaacag aaactgttgg tgcgttaaag gtgattttcg ctaattctac tgccagtttg
    59581 tcggctgctg gtttcatcag cgcacagtga gacggtacgc tcactggtaa cggcagcgcg
    59641 cgttttgcgc cagcagcttt acaggcagcg ccagcacgct caaccgcttc tttatgaccg
    59701 gcaataacca cctgtcccgg agagttaaag tttaccggag aaacgacctg accttctgca
    59761 gcttcttcac acgctttcgc aataggcgca tcatccagac cgatgattgc cgccatagcg
    59821 cccgtgcctt ccggtacggc ttcttgcatg aacttaccgc gcatctcaac cagacgcacc
    59881 gcatcagcga aatcaatcac gccagcgcaa accagcgcgg agtattcccc caggctgtga
    59941 ccggccatca ttgccggtgc tttaccgccc tgctgctgcc atacgcgata cagcgcaaca
    60001 gatgcagtca acagcgctgg ctgggtttgc caggttttat tcagttcttc agctgacccc
    60061 tgctgggtca gcgcccacag gtcgtagccc agcgccgcag aagcttcagc aaacgtttct
    60121 tcgacaattg gatagctcgc cgccatatca gccagcattc caacggtttg agaaccctgt
    60181 ccagggaaca caaatgcaaa ttgcgtcatg ttttaatcct tatcctagaa acgaaccagc
    60241 gcagagcccc aagtgaatcc accgccaaag gcttcaagca gaaccaactg ccccggctta
    60301 atgcgcccgt cgcgtacagc ttcatccagc gcgcacggga cagaggccgc agaggtatta
    60361 ccgtggcgat ccagcgtcac cacgacattg tccatcgaca taccgagttt tttcgccgtt
    60421 gcactgataa tacgcaggtt agcctgatgc ggaaccagcc agtccagttg agaacggtca
    60481 agattattcg ccgccagcgt ctcatcaacg atgtgcgcca gttccgttac cgcaaccttg
    60541 aagacttcgt tgcccgccat cgtcagatga attgaattct ctggattcac gcggtcggcg
    60601 tttggcagcg tcagcaattc accataacta ccgtcggcat gcagatgggt ggaaatgatt
    60661 cccggctctt cagaggcagc cagcaccgca gcgcccgcgc catcgccaaa aataataata
    60721 gtcccacgat ctgttggatc gcaggtgcgc gccagtacat cggaaccgac gaccagagca
    60781 tacttcaccg ccccagattt cacgtattga tcggctacgc ttaatgcata ggtgaaacct
    60841 gcacaggctg ctgcaacgtc aaatgccggg caacctttaa tgtccaacat gctttgaatc
    60901 tgacaagctg cgctcgggaa agcgtgcgta gcagaagtcg ttgccacaac gatcaggcca
    60961 atctggtctt tctcaatgcc cgccatctca attgcgcgtg tcgccgcttc aaagcccatg
    61021 gttgaaacgg tttcgtttgg cgcggcaatg tggcgttcgc ggataccggt acgagtgaca
    61081 atccactcgt cagaggtgtc caccattttt tccaaatcgg cgtttgtccg cacttgttcg
    61141 ggcagatagc tgccagtacc aataatcttc gtatacatgt acgctcagtc acttttcggt
    61201 tatataccgt cacttgcaaa ctgcgagttc gctggcagcg tcctgctacc gcagagttcc
    61261 gcttttgcca ccgtccagca gctcaaaacc agctgggtat acagattcca ggcgagcggc
    61321 aattcgctga ggaacttgtc gctgcaccgc ctgcactgcc tgttcaatcg cgaccgcaaa
    61381 agctcgctga ttggctgcac catgactttt tatcaccgtg ccgcgcaatc ctaacagaca
    61441 ggcgccgtta tactggtcgg ggttgaggtg actgaatcgc ctcgtcaggc tcttttgtag
    61501 ccaacgcttt aataacagta gccaccacga ccgttttttc ccttcaccct gagatttcag
    61561 cagagaaagg aacatcctga caacaccttc catcgtcttt aacgtgacat ttcctgtaaa
    61621 gccgtcacaa accagcacat ctgtcttgcc agttaacaac tcattggctt caagatagcc
    61681 gatataattg atagaaggga ttgttttaag caccgctgag gcatcccgaa tactgtcaag
    61741 accctttact tcttcttcac caatattgag caacgccacg cgaggattgg gaatttccac
    61801 cacctcttca gccagaactg agcccataat ggcaaattgc accaacattg tgctgtcaca
    61861 atcgacgttg gcccctaaat cgaggaccac cgttttgccc ttttgctgat gtggtaatac
    61921 cgtcaccagc gccggacgct caatcccctc caggggcttg agtaataatt ttgccagccc
    61981 catcagtgcc ccggtattac cggcactgac acaggcttgc gctcgacctt ctttcaccag
    62041 ctccagggcc atacgcattg aactcccacg gctggcgcgg atagcttgcg aaggccgggc
    62101 atcactggcg ataactgact gcgcaggaat aatctgcaga cgcgaacgtt gttcaaagtc
    62161 agctttagca agtaatggcg tgatggcgtc gggattgccg actaaaagaa gagtgagttg
    62221 cgaattagaa ttcagtgcct gcaatgctgc aggcactgtc acggaagggc caaaatcccc
    62281 tcccatgaca tctaacgcca gggttagacg tgtcaaggta tcgtcgccgc ccggtttggt
    62341 ctttccccag ttgcctgggg aaaatcctca ctaagcttca tcacgcagac gcgtgattac
    62401 ttagcgatga ccttgcggcc gcggtagtaa ccgtcggcag tgatgtggtg acgcaggtgt
    62461 ttttcaccag aagttttgtc tacagacagg ctggtgactg cggtcagcgc gtcatgggaa
    62521 cgacgcatgc cacgtttgga acgggttggt ttattctgtt gtacggccat ggaccttact
    62581 cctcaattac ggtttatccc cgatccaacg gggagcacca attacttacg ctttaagctg
    62641 gctaatacgg caaatgggtt tggcttttgc acttcttcag gcagttcacc aaagaccatg
    62701 tccgcttcgg acacttcaca gtgttcagaa tcatgcaccg gaactaccgg caaggcgagg
    62761 atgatttcat cttcaaccat tgcaagcaga tcgatttcac cgaattcgtt aacctcaatc
    62821 ggttcatacg cttccggcag tgcttcagcc tgttcgtctg aacgcacagg actaaaacaa
    62881 tacgttgtgt agacctgatg agtaaacggc ttcccgcaac gctgacactc gagcgttacc
    62941 gtcaccttcg catcgccgtt taacactgcg agacgttggt tatcgatagc gaacgacatg
    63001 gagcattcca catcactgtc cacactgact acggattcgg cgacgcgctc aacctgatca
    63061 ggggtataga taccctggta atcaaggcgt ttttgagccg tacgaaccgg atcgagagtc
    63121 aggggtaatt ttaccttttg catagggcgc gcatattaac tttgtaacgt catagagtca
    63181 aagaaaaagg cagcctgtgg ttgccttttg ccaataattc gcacacattg cgggttactg
    63241 ctttattttc gttcagtgct gataacgaac atcaacctgc aaaacgaagc gcatagttta
    63301 aaatggctat catcaatacg ctatatctga tggaaaaaaa tatgcctaaa cttattttag
    63361 cctccacctc gccatggcgt cgcgctcttc tggaaaaact gcaaatctct tttgaatgtg
    63421 cagcacctga ggtcgacgaa accccacgca gcgacgaatc accgcgacag ttggtgcttc
    63481 gactggcaca agaaaaatcg caatctctgg cgtcacgtta tccggatcat ttaattattg
    63541 gttcagacca ggtatgtgtt cttgatggcg aaatcaccgg taaaccgtta acggaagaaa
    63601 atgcccgtct gcaattacgc aaagccagcg gcaatatcgt caccttctat actggactgg
    63661 cgttgtttaa ttcggcgaat gggcatctac aaacagaagt ggagcctttt gacgtccatt
    63721 tccgtcacct aagcgaggcg gagattgata attacgtgcg taaggagcat cccctgcact
    63781 gcgcgggtag ctttaagagt gaaggatttg gcattacgct gtttgagcgt ttagagggac
    63841 gtgaccctaa cacgctggtt ggtttaccgc taatcgcgct gtgtcagatg ttacgtcggg
    63901 aagggaaaaa cccgctgatg ggatagttgt ttgcgccgga tgcggttacg tcgcatccgg
    63961 caatcggtgc aggcggcctg atgcggcgta aacgccttat caggccaacc gcccattatc
    64021 aggcttatat gcttgtatta gcgcgcgtta cgcagctttt gcaaacaacg cttcaaacct
    64081 tcatccatcg gcgcttcgat acgcatcacc tcaccggtcc ccggatgggt aaacttcaac
    64141 gctgcggcgt gcaggaacag acgatttaat cccgtgcctg cttcagtgag ctgtctgtca
    64201 aattcacggt caccgtagcg atcgtcaaag gcaatcggat gacccgcata ctgtgtatgc
    64261 acacggatct gatgagtacg tccggttacc gggctacaac gcaccagggt ggcaaatgca
    64321 tagcgttctt ccactttaaa gcgtgtttcc gacggtttgc cttcctgact gacacgcacg
    64381 atacgttcgc cgctttgcag aatatttttc aataacggcg cttgaacgct cttcacatgc
    64441 gactgccact gaccgcgcac cagcgccagg taatcttttt gcattccttt ttcacgtaat
    64501 tgctcatgca gagaacgcaa cgccgagcgt tttttcgcta ccagcaacac acctgaagtg
    64561 tcccggtcga gacgatgaac cagttcaagg aaccgcgctt ccgggcgcaa cgcccgcaaa
    64621 ccttcgataa cgccgaagct taaaccactg ccgccatgta ccgccgtacc ggaaggttta
    64681 ttcagcacca ggatatgatc atcttcatat aagatgacgt ccgccagcgc cgccaccttt
    64741 tgcagatgtg gcgaaaccgc ctcttcttcc cgctcagcaa cgcgaaccgg tgggatacgc
    64801 acctcatcac ccgcttcgag tttatattca ggcttaatac gttttttgtt cacccgcact
    64861 tcacctttac gcaaaatacg gtaaatcata ctttttggta cgcctttcaa ttgggtacgc
    64921 aaaaagttat cgatacgttg ccccgcttcg tcagcggtga tagcaacaat ttttacggat
    64981 ggagtctctg ttttcatggt gggcgattct aattagccaa caggattcgc gccactcatt
    65041 tttctatgct tatatttagt tttgcacctt attacttcac tacgtgatca ctttattgat
    65101 ggttattaaa ccaatcacca gcaagaagtg aaaaaactgt gagtaagcgg gtgataaatg
    65161 gtaaaagtca tcttgctata acaaggcttg cagtggaata atgaggccgt ttccgtgtcc
    65221 atccttgtta aaacaagaaa ttttacggaa taacccattt tgcccgaccg atcatccacg
    65281 cagcaatggc gtaagacgta ttgatctttc aggcagttag cgggctgcgg gttgcagtcc
    65341 ttaccggtag atggaaatat ttctggagag taatacccag tctgtttctt tgataattgc
    65401 gctgtttttc cgcatgaaaa acgggcaacc gacactctgc gcctctttga gctgacgata
    65461 accgtgaggt tggcgacgcg actagacacg aggccatcgg ttcacacccg gaaaggcgtt
    65521 actttgcccg cagcttagtc gtcaatgtaa gaataatgag taagttacga tgaaaagaat
    65581 gttaatcaac gcaactcagc aggaagagtt gcgcgttgcc cttgtagatg ggcagcgtct
    65641 gtatgacctg gatatcgaaa gtccagggca cgagcagaaa aaggcaaaca tctacaaagg
    65701 taaaatcacc cgcattgaac cgagtctgga agctgctttt gttgattacg gcgctgaacg
    65761 tcacggtttc ctcccactaa aagaaattgc ccgcgaatat ttccctgcta actacagtgc
    65821 tcatggtcgt cccaacatta aagatgtgtt gcgtgaaggt caggaagtca ttgttcagat
    65881 cgataaagaa gagcgcggca acaaaggcgc ggcattaacc acctttatca gtctggcggg
    65941 tagctatctg gttctgatgc caaataaccc gcgcgcaggt ggcatttctc gccgtatcga
    66001 aggcgatgac cgtaccgaat taaaagaagc actggcaagc cttgaactgc cggaaggcat
    66061 ggggcttatc gtgcgcaccg ctggcgtagg caaatctgct gaggcgctgc aatgggattt
    66121 aagcttccgc ctgaaacact gggaagccat caaaaaagcc gctgaaagcc gcccggctcc
    66181 gttcctgatt catcaggaga gcaacgtaat cgttcgcgcc ttccgcgatt atttacgtca
    66241 ggatatcggc gaaatcctta tcgataaccc gaaagtcctc gaactggcac gtcagcatat
    66301 cgctgcatta ggtcgcccgg atttcagcag caaaatcaaa ctgtacaccg gcgagatccc
    66361 gctgttcagc cactaccaga tcgagtcgca gatcgagtcc gccttccagc gtgaagtccg
    66421 tctgccatcc ggtggttcca ttgttatcga cagcaccgaa gcgttaacag ccatcgacat
    66481 caactccgca cgcgcgaccc gcggcggcga tatcgaagaa actgcgttta acaccaacct
    66541 cgaagccgcc gatgagattg ctcgtcagct gcgcctgcgt gacctcggcg gcctgattgt
    66601 tatcgacttc atcgacatga cgccagtacg ccaccagcgt gcggtagaaa accgtctgcg
    66661 tgaagcggtg cgtcaggacc gtgcgcgtat tcaaatcagc catatttctc gctttggcct
    66721 gctggaaatg tcccgccagc gcctgagccc atcactgggt gaatccagcc atcacgtctg
    66781 cccgcgctgc tccggtaccg gtaccgtgcg tgacaacgaa tcgctgtcgc tctctattct
    66841 gcgtttgatc gaagaagaag cgctgaaaga gaacacccag gaagttcacg ccattgttcc
    66901 tgtgccaatc gcttcttatc tgctgaatga aaaacgttct gcggtgaatg cgattgaaac
    66961 gcgtcaggac ggtgttcgct gcgtgattgt gccaaacgat cagatggaaa ccccgcacta
    67021 ccacgtgttg cgcgtgcgca aaggggaaga aacgccgacc ttaagctaca tgctgccgaa
    67081 gctgcatgaa gaagcgatgg cgctgccgtc tgaagaagag ttcgctgaac gtaagcgtcc
    67141 tgaacaacct gcactggcaa cctttgccat gccggatgta ccgccagcgc caaccccagc
    67201 tgaacctgcc gcgactgtcg tagccccagc accgaaagct gcaacggcaa caccagcagc
    67261 tcctgcacaa cctgggctgt taagccgctt cttcggcgca ctgaaagcgc tgttcagcgg
    67321 tggtgaagaa gccaaaccga ccgagcaacc aacaccgaaa gcagaagcga aaccggaacg
    67381 tcaacaggat cgtcgcaaac ctcgtcagag caaccgccgt gaccgtaatg agcgccgcga
    67441 cacccgtagt gaacgtactg aaggcagcga taatcgcgaa gaaaaccgtc gtaatcgtcg
    67501 ccaggcacag cagcaaactg ccgagacgcg tgagagccgt cagcaggctg aggtaacgga
    67561 aaaagcacgt accaccgacg agcagcaagc gccgcgtcgt gaacgtagcc gccgccgtaa
    67621 cgatgataaa cgtcaggcgc aacaagaagc gaaggcgctg aatgttgaag agcaatctgt
    67681 tcaggaaacc gaacaggaag aacgtgtacg tccggttcag ccgcgtcgta aacagcgtca
    67741 gctcaatcag aaagtgcgtt acgagcaaag cgtagccgaa gaagcggtag tcgcaccggt
    67801 ggttgaagaa actgccgctg ccgaaccaat tgttcaggaa gctccagctc cacgcacaga
    67861 actggtgaaa gtcccgctgc cagtcgtagc gcaaactgca ccagaacagc aagaagagaa
    67921 caatgctgat aaccgtgaca acggtggcat gccgcgtcgt tctcgccgct cgcctcgtca
    67981 cctgcgcgtg agtggtcagc gtcgtcgtcg ctatcgtgac gagcgttatc caacccagtc
    68041 gccaatgccg ttgaccgtag cgtgcgcgtc tccggaactg gcctctggca aagtctggat
    68101 ccgctatcca attgtacgtc cgcaagatgt acaagttgaa gagcagcgcg aacaggaaga
    68161 agtacaagtg cagccgatgg tgactgaggt ccctgtcgcc gccgctgtcg aacctgttgt
    68221 tagcgcgcca gttgttgaag aaatggctga agtcgtagaa gcccccgtac cggttgccga
    68281 accgcaaccg gaagtggttg aaacgacgca tcctgaagtg attgccgccg cggtaactga
    68341 acagccgcag gtgattaccg agtctgatgt tgccgtagcc caggaagtcg cagaacacgc
    68401 agaaccgatg gttgaaccgc aggaagagac ggcagatgca gatattgaag aagttgccga
    68461 aactgctgag gttgtggttg ctgaacctga agttgttgct caacctgccg cgccagtcgt
    68521 cgctgaagtc gcagcagaag ttgaaacggt ggccgcggtt gaaccagaga tcaccgttga
    68581 gcataaccac gctaccgcgc caatgacgcg cgctccggca ccggaatatg ttccggaggc
    68641 accgcgtcac agtgactggc agcgccctac ttttgccttc gaaggtaaag gtgccgcagg
    68701 tggtcatacg gcaacgcatc atgcctctgc cgctcctgcg cgtccgcaac ctgttgagta
    68761 ataattagct caaagtaatc aagccctggt aactgccagg gcttttttat ttcatgtttg
    68821 aatcaacata ttaaaattca tcaaatggca tccttgctaa ccaacaatgc aaaataggca
    68881 aaaaaaatgc ccgccccggc gatcccagag cgggcagaca aaacataccc agtttcatga
    68941 tatgttttaa agcgagctta tttgttgagc tggaagagcg acaatccctg catatcggta
    69001 aatgctttat acgatgcctg caatgccgtt tgctgcatga tgtaagatga aatagttgca
    69061 ttccagtcca catcaaccag atcgctcatc tgctgcgttt gccctaaagc gcgatcgcta
    69121 cctaaggaat ccagcgactc cagtttgttc agctgcgtgc ctaattccgc gcgaacggta
    69181 aggacattat tcagcgagtt tttcagtcca cggttggttt tatccaatgc cgcagcggcg
    69241 atttctttat ccgcttcgct atccgcgacc ggcgttttca gggctgcgat ggcgctatcc
    69301 agcatggcaa aaagattggt ttcagaagcg ctaccgtctg gttccgctac cgcgttactg
    69361 gtaatactgt cgaaaatttt gtcgcccgta tgtcctatca ccatcgaacg cgaagcatcg
    69421 acctgttgct taatactttc tgttccgcca acataatcac cgttagcctc actaaacggc
    69481 gcagtctctg ttttatatgt agcgcccatt accgtcagtg gtgttcgcca gattcagcaa
    69541 ctggtcacga agcccctgaa tatccgtagc cagcgaggcc cggtcatcgt cactcaaggt
    69601 gccattgctg gcgtagacaa ttttttcctg agcattctgg atagcagtgg tgacctggct
    69661 aagtacactc tcttccagtg acactttttg agtggcgaaa gtacgtgcca gcgtgtactg
    69721 gctgttttgc gcctgtgcct gggagagaac aacagcttgt gatgcagcaa tgggatcgtc
    69781 tgaagggtta acgactcgct tacccgtcga catctgctcg ccgtacttca tccattctgc
    69841 ctgagaattg gtgatgccgc gcatgttttg ctggtacatc atctgggtac tgaaacgcat
    69901 tttatctccc cttagcgaat gttaatcagc gcatcaaaaa tcgcgtttgc cgtctgcaga
    69961 acctgcgcat tcgccaggta atactgctga aaacgttgca gatttccgta ctcctcatcg
    70021 agattgacac cggaaatcga ctgctgctga ttggaaagct gcgtcaccac attaccttgc
    70081 gtggtgctac tggttttcaa cgtcgcggtt ttattaccga tatcactcac taacgaggca
    70141 taagcgtcgt taaaggattt cgcaccgccc accgttttac tgttgctttg cagatccagc
    70201 agggcctgac cgttgcggtt gtcgctatca cccgcatctt cttcgctcgc catcgcgatt
    70261 ttcgcttcgt cggtgattaa tacatccatg ttgacgatgg cgtcacttac tggtttcagc
    70321 gtgaagctgt cgttaacggc aggcgttcct gtaaacgtca actccagacc atcaaatgcc
    70381 actttaccgt tggcatctgg tgtcgccgta aaagtggtat tgctggcaag gcgggtgacc
    70441 tgccactgat tattatcgaa cgagattttg taatctgtcg ccagtaccac ggaggcatca
    70501 gttaccgtgg caccgatcgc aacgtcacct ttgtttttcg tgttttgcag aaccgcgggc
    70561 ttaccgatag taaagaaatc ttcaccagca tcgccgttag catcaaatcc ggctttgtgt
    70621 tggctgttga aagcctcggc aaatgccagc gccagttgtc caagcgtatt acgcgtctgg
    70681 tccagatcct gagaacggaa tgtcagaatg ccgcccagcg acccggtatt cagtaatttc
    70741 tccgggatct caatattgcc tgccgtccca tcaacgtaag cgacagtcgt acgagaaggg
    70801 tcggcgctgg aaggaactgc cgccagttgc cgcgccgtac ttccctgaac cagtgagtaa
    70861 ccattggcat cgtgatgtta taagtaccgc catcctgaac gctgacttct acgccaacaa
    70921 tctggtttaa ttcgctcacc agttgatcgc gttgatccag cagattgtta ggtgacgccc
    70981 ctgcccccac gcctgtcagg cgcgagattt gatcgttcag gctggcaatt tgtttagcgt
    71041 aattgttgat ctgatcaacg ctggcaccta tcgcgatatt gacctgtttg tcctggtcgc
    71101 gcagatattg atcggtggtt ttaaactgat tcaccaaccc ttctgatttc ccaatcagcg
    71161 cctggcgcgc cgccgggtct tccgcgttac tcaccagcgt ttgcaggctg gtgaagaaat
    71221 cctgcatctg tgttgccagc gaagaggtac tggtggagag catattgtcg attttcgaca
    71281 tctgctcata gcgggcggtc aggccgctac tttgcgtctg cgcagcacgt aactggttgg
    71341 taataaacgt atcatactca cgctgcacac cagaaacgta gacgccattg ccaacccagc
    71401 cgccagcgcc caacgtgcta ttggcctgcg ccataatagt ggtttggcgg gtatatccgg
    71461 caacgttata gctggagata ttattacttg ccgtatttaa cgccgcctgg gccgcgttca
    71521 gtccgctcat ggcgttattg atcaagctgg acatggaggt tccttataag ccttcaatta
    71581 cagagtatta tcggcagcga cccgccggac ttgagttatt cagaacagat tatcaatgtt
    71641 catgctgtag gttttgctca ccttgtcgct tatcgatttc atctgctgga tcatgttggt
    71701 gagtttacgg gcatagtgag gatcggtagc atagcccgcg tcctgtagga cctgcgcccc
    71761 ctgttccgca ctcgcggcgg tcgtcacggc ggcgtagcgc gggttacgtg ttaacagccc
    71821 aacgtaatcc gacaatgctt ccagatacga gctgtagacc cgaaacttcg cttttacttt
    71881 cttcgcttcg ccgttttcat attcagtcgt ggtgatttca gtgacttgcc ctttccagtt
    71941 gccagaggct ttgacaccaa acaggttata gcttggctcg ccgttttcgc ggcggatttg
    72001 ccgttgcccc caaccagatt caagcgcagc ctgagcgagg atcaaatgat gtggcacacc
    72061 gctttgctgg cttgccagtt gggcgggcag cgagagttgc gcgaggaatg ctctactgtc
    72121 acccggcagc gaatcatcgt agttacgtgg tacggccttt tgcaccagct gcgaaagcgt
    72181 ctggttttga taacgcacca cggtttcgag cgggaatttc atcggtgctg ctggcgtgga
    72241 ctcctctggc aatggttgtt ctggcgtcat ctgtttaacc atcatctctg caagccccag
    72301 acctttgccc gccgtcatct gttgggcaat ttgctggtca tacatactgg tatacagacg
    72361 agtgtgctcg ctgctgaaca ggccatcttt tggtaaagcg tcgcgcatgc ttttcaacat
    72421 catctgcacg aacatccctt ccacctgacg ggccaccgga cggatatttg ccgccggatc
    72481 ttcgctcgct ttcgccttta gttcgttgag tgattgcgca tcccaggccg cacttgccag
    72541 tagtttgctg tcgctgatca tcagatgatt tccagttttg cccgcagaca tcccgcactt
    72601 tgcattgatt gcaaaataga catcagatcc atcggcgtag cgcccagcgc attgagcgca
    72661 cgcaccacgt tattgaggct ggcgctggaa cgtacgcttt gcagcgaacc gccgctctgg
    72721 cgtaaatcga tctgcgtttg tggagtaaca acagtctgtc caccaccaaa cggtgtatct
    72781 ggctggctga cattggcctg acgattaact gttactgaga gatttccctg cgctaccgcg
    72841 cagctgtcga gggtcacttc gcgattcatc accaccgaac cggtgcgcga gttaatcact
    72901 actttagcgt cctgcggggt gacattaacc tgcatattct ggatatcggc aaggaaacgg
    72961 acctgggaac tgttgccact tggtacgcgc acctgaatag tccgcgcatc taacgcggtg
    73021 gcgctgccat atccacgcac gcggttgatg gtgtcagcga tttgctgcgc catgctgaaa
    73081 tcttcgtcgt taagttgcaa attaagggta ttcccgacgc caaactggct gggcaattca
    73141 cgttcaataa ccgcaccatt ggtgatccgt ccaccgttca gttggttaac ctgaacactg
    73201 ctaccgcctg cggaggctcc tgcgccgcca accagaatat tgccctgcgc cagcgcatac
    73261 acctgactgt caacgccctt aagcggagtc atcaacaacg tacctccacg caagcttttg
    73321 gcattaccca tggaagaaac caccacatcg atggtttgcc cctgacgtcc aaacggagga
    73381 agtgacactg tcaccattac cgcagcgacg ttttttagct gcatattggt gcccgtcgga
    73441 acggtaattc ccagctgtga gagcatgtta ttaagcgttt gtgtggtaaa cggcgtctgg
    73501 gttgtctggt caccagtgcc atccagcccc actaccaagc catagccaat cagtgagttt
    73561 tgccttaccc cctgaacact ggtgagatcg cgaatacgct cagcctgagc cgccgtcgta
    73621 accagtagaa gaattaatgc agagagaaat ttaatcacca cagcctcact tacattggcg
    73681 acaggttaag gaagaaacgc tgcaaccagc ccatattttg cgcttcgtta atgtagccat
    73741 tgcctacgta ttcaatacgc gcatccgcca cctgagtaga cggtacggta ttgctgccgc
    73801 tgatagtgcg tggattaacc acgcccgaga agcgaataaa ttcggtaccc tgattaatgg
    73861 caatctgttt ttcacccacc acatgcaggt tgccgttgac cagtacctgg tcaaccgtca
    73921 ccgtcaacgt gccgctaaag gtattgctgg cattggcccc gccctttccg ttgaacgtgt
    73981 taccaccgga ggcttcgaca tcggcacgag cgttaccaaa cagcccctgc aaatagcgcg
    74041 gcacagtatc aaagccaaaa ttagttttac cgtcacggct ggcattcgca gaggagcttt
    74101 tgctggcgct gacgttctcc tgcaacacga tggtcagcgt atcgccaata ttgcgtggtc
    74161 gacgatcttc aaacagcggt tgatagccat agttaatcgg ctgagcagac tggaaaatag
    74221 aaccgttggc gacgggcgtc ggaccgggaa ccggttgtgc actggtcgcc ccctgcacca
    74281 gcggcgtgga gggtatccag gcgcagccgg ttagtgaaag caccaacaag ctggaaatgg
    74341 cataagtatg cgcagcgttt ttttgcatgg ctatcatctt caaaaatcag taaaccggtg
    74401 aacctgccac cggttatgcc ttagagttgc gtcagttttt gcagcatctg atcggtggtg
    74461 gacaccgctt tactgttgat ttcgtaagcg cgttgcacct gaatcatatt gaccagttct
    74521 tccgccacgt tgacgttaga cgtttcaaca tacccttgat acagcagtcc cgcgccgttc
    74581 aggcccggcg tgctttcgtt cggtgcaccg gaggattgcg tttcggtgta gaggttttcg
    74641 ccaatgctct ccagtccggt atcattcata aaggtggtga gattgagctg cccaacctga
    74701 accggagctg cctggccttg ttgggttacg ctgaccacgc catcacgacc gatggtgata
    74761 cttaacgcat tcgccggaat ggtgatcgct ggctgcacct gaaaaccacc agccgtcacc
    74821 agctgcccgt tctgatccac ctggaaagag ccgtcacgga tataggctga tgagccatct
    74881 ggcaacatta cctggaaaaa gccctgccct ttaatcgcga catctttgct gttattggtc
    74941 tgcgacagat tgccctggct gtgtaagcgt tcagtggcga ccgggcgtac ccccgtgccg
    75001 atttgtaatc cggagggtaa ggtggtttgt tcggaagact gtgcccccgg ctggcgaatg
    75061 gtttgataaa gcagatcttc aaacaccgcg cgctgacgct taaaaccgtt agtactgacg
    75121 tttgccaggt tgttggcaat gacgtccata ttggtttgct gggcgtcaag gcccgttttg
    75181 gcgatccata atgaactgat catataatgt cctgtagtgg attgctgaca agcagagcag
    75241 aagcacgcga cagtgtgaat ttcccctcac cctaaccctc tccccaaagg ggcgagggaa
    75301 tagtccgcgc gcttttattg caatgtcatc aatcgttcaa ttaactcatc gacagcagtt
    75361 ggttggcacg gcctgcgtta tcatcgacgc tgctgatcac cttcatctgc atttcaaaac
    75421 gccgcgcgct ggcaatcatg tcgctcattg ccgcaacggc attgacgtta ctgccttcca
    75481 gaacccccga catcacacgc aaggttggat ctgcctgcag taccggccct cgcgtggcct
    75541 gggtttctgc gcttaaacga aaaatgccgt cgtcaccgcg ctgcacttcg ctgcccgtgg
    75601 ctttcaccag tttcagacgc cctactggcg caactgtatt tgccggatcg cccggattga
    75661 gcgccgagat tgtgccatcg gcagcaatcg tgatttccgc cccttccggc acagcaattg
    75721 gccccgcctc gcctatcacc ggatgcccct gaattgtcag ttgcccggtg ggatcaacct
    75781 gaatgctgcc attacgcgta tacccttcgc tgccgtcagc ggtctgcacg gccagccatc
    75841 ctgctgcaac gcaacgtcca gcgggcgaga ggtgtaatcc atcttgccgg gcgtcatgtc
    75901 tgcacccggt gttgacgccg ttaccagcgt gcgcgtgggc agtgaaagcc cttccaccgg
    75961 cactgcgcgc aaggcgttta actgcgcgcg aaaaccgggc gtagaggcat tggccagatt
    76021 actggcggtt accgcctgtt gattcagcgt ctggctggct gctcccatcg cagtataaat
    76081 tgcgtgatcc attgagctat cccgtcagcg attagcgtaa gttaacccgc gtgttgagga
    76141 tctggtcctg ggttttgatg gtctgggcgt tagacttata gttacgctgg gcaacgatca
    76201 tattgaccag ttctttactg agatcgacgt tggacgcttc cagcgcaccg ttggtcagag
    76261 tgccaaagtt tcccgtcccg gctgtcccca acagcgccac gccagaagat tgcgtcgcag
    76321 accagacgtt gtcgccttcg gatgccagac cttcgttgtt ggcaaagttc gccagtacaa
    76381 tctgccccag cagttgggtt tgttcgttgg aattgttgcc gacaaccgta ccgtcatcat
    76441 tgatttgata actcaccaga tcgcccggtt tgtagccatt ctgggtggtt gccacaatgt
    76501 tgttagcgcc ggtattttgc tgcatggagt tgaggaagct cagactaaac gtggcgggtt
    76561 ctgcaccgtt aattgcgccg gttgcgatat tattcgccat cgcaccatcc actaatgtgc
    76621 cattagcatt aaattccagt gttgtcgctg tcttcgcaat gctgtttgga tcactgctat
    76681 cctgggtgta gacctgccag ttattatccc cggtcttcac aaaatagacg ctcatgtcat
    76741 gagcattacc ctgactgtcg aaaacagtca ccgaaccttt tttgttatag ctatccgcat
    76801 tgctggcgct gaatggcgta acagtaggaa gcggatcact ggaattcagg ttgatctgca
    76861 tcgacgccgt ggtggtagtt ttcgctgcca tcagggtatt cgggatcgaa atattggtcg
    76921 gattcgcccc ttgctgaata gtcggcggcg taccggttac cgggtaaccc gtcagctgta
    76981 aaccttgcgt attcaacagg ttacggtttt catccagctt aaattgtccg ttacggctgt
    77041 agaacaccga accgttgctg tctaccagac ggaaaaaacc gttctggctg atagcaacgt
    77101 ccagacctcg cccggtgttg gtggtcgtgc catcggtaaa gtcctgagtg ataccggcaa
    77161 cttttacccc cagtcccact ttcgaaccgg caaacatatc ggcaaaagag gccgtgcctg
    77221 atttaaagcc gtaggtggcg gagttggcga tattgttgcc aataacatcg aggttggtgg
    77281 cggcagcgtt taatccgcta accgcttgag aaaaggccat gactgactcc tgaaatgtga
    77341 aggcttaaat tatctgccgt acttcgtcga gggtggtggt gccgtaagtg ccgagatcca
    77401 gcgtattacc gctgttgccg cggatcacac cctgcaccag agcaaactgc agcggctggg
    77461 caaccagttg tgtaccaccg ttactggcgc taatcgctac attgtaagaa ccgttcggcg
    77521 cagtgctgcc atcagtcaac gaaccgtccc aggtgaaact gtgaactccg gcggtcagtt
    77581 caccaatatc aatggtgcgc acaaccgcgc catttttatc ggtgatggtg gccgtaactt
    77641 tgtctgccgc ctgttgcagc tcaacaccaa acggcgtggt cgtggtcaca gccccttctt
    77701 cactgccggt tcccgcaaga acagttgtgc cggggatcat cacgccgtga ccgatcaggt
    77761 tactggcctg taacgactgg ctgttatcaa tctgtccgga aatagatccg agcgtggtat
    77821 tgagtttttc aatcccgctg accgtgctga tttgtgccaa ttgcgacgtc agctcgttgt
    77881 tttccattgg attggtcggg tcctggtttt tcagctgcgc caccagcaaa gtcagaaaac
    77941 tgctttgtaa atctgcggcg ttgctgcccg tgagcgaact actgctggtg gtactgacgc
    78001 cggtatttgt cggatcggtg gtggttaccg caatggacat agctttctcc tttattgacc
    78061 gagcgtaagg gttttcagca tcatgctttt caccgtgttg agcacttcaa cattggcctg
    78121 atagctgcgt gacgccgaca tggtgttaac catctccccg acgacatcaa cgttcggcat
    78181 ttttatgtag ccctttgcat ctgccagcgg attacctggt tcataaacca gtttgtccgg
    78241 ggcctgactt tcttaacatc ggcaaccttt acgccgcctg tcgcagcacc tggtgcagcg
    78301 ttaacctgga ataccacctg ttttgcccga tatggctgtc catcaggacc ggtcacgcta
    78361 tcagcatttg ccagattact ggccgccacg ttcaggcgct gggactgggc agttaacgcc
    78421 gacccggcga tatcaaaaat attcagcagt gccatccgtt aatttccgct ctgtaaaacg
    78481 ttcatcatgc ctttgatttg cccgctcaac gcgctaaggc tcatctggta ttgcaggctg
    78541 ttatcggcaa actgggtgcg ttcgcgatcc atatcgacgg tattaccgtc aagcgaaggc
    78601 tggtccggaa tacggtattg cagttctgcg gtaggaggcg tcagcgcctg cgccgggatg
    78661 tgctgcgttg aggtcatcgt cagtgcaacc acactggttg catcccgtcc acgttgcatg
    78721 acttttttaa gttcactggc aaaatcgata tcgcgcgcct gataaccagg ggtatcggca
    78781 ttggcgatgt ttgctgccag cacttcctga cgctgggcgc gcagattgag cgcctcttgt
    78841 tgaaaacgta aggcggcgtc gagcttatcg agcatatctc ctccgcaggt atcaaaattc
    78901 tgccatcagc ttaaatgcct ttacgcgcgc gttatcggcg gaataaacgc aaaatgggtc
    78961 gctatttatg ccgttgatgg tcattgcgga caggtacaat tcacgttgta gaaatggctg
    79021 ggggcgaaaa tgctgacaat aaaacgtagc gtggcgatca tcgcgatact gttcagtccg
    79081 ttaagtacgg cgagcaatct cacatcgcaa ttgcacaact tttttagcgc ccaactcgcg
    79141 ggggtaagtg atgaggttcg tgtttctatt cgtacagcgc ccaatctact accgccatgc
    79201 gagcagccat tgctttcgat gagcaataat tcccgcctgt ggggcaatgt gaatgtgttg
    79261 gcacgctgcg gtaacgacaa acgatattta caggttaatg tgcaggccac aggaaattat
    79321 gtggttgccg cgatgcctat tgtgcgggga ggaaagctgg aagctggcaa tgtcaaactg
    79381 aaacgtggac gcctggatac cctgccaccg cgtacggtgc tggatatcaa tcaacttgtt
    79441 gatgccgtta gcctgcgcga tctatcaccc gatcaaccta tccagttaac ccagtttcgc
    79501 caggcatggc gggtaaaagc gggacaacgc gtcaatgtga tcgccagcgg tgatgggttt
    79561 agcgccaacg cagaaggtca ggcgctgaac aatgcagccg tcgcacagaa tgcgcgggtg
    79621 cgcatggtat cggaacaggt agtcagcggc gttgttgatg cagatgggaa tattcttata
    79681 aacctgtaag ctgttaaaga ttacccgtcc cttgccgata aataagcaac acatgataaa
    79741 agcgccctca atgaggaata aaccatgagt attgatcgca cttcgcctct gaagcctgta
    79801 agcaccgttc aaccgcgcga aaccaccgac gcgccggtaa cgaacagccg ggcggcaaaa
    79861 acaaccgcct ccaccagcac cagtgtgacg ttaagcgacg cgcaagcaaa actgatgcaa
    79921 cccggcagca gtgatatcaa tcttgaacgt gtcgaagcgt taaaactggc gattcgtaac
    79981 ggtgaactaa aaatggacac cggcaaaatt gccgatgcgc tgatcaacga agcgcagcaa
    80041 gacttgcaga gtaactgatc gtatgacacg tcttgcagag atcctcgacc agatgtccgc
    80101 tgtgcttaac gatctcaaaa cggtaatgga tcaagagcag caacatctct ctatggggca
    80161 gatcaacggc agccagttgc aatggattac agaacaaaaa agctcactgc tggcgacgct
    80221 ggattacctc gaacagttac gcaagaaaga acccaataca gcaaatagcg ttgatattag
    80281 tcaacgctgg caggaaatta ctgtgaaaac gcagcaacta cgccaaatga atcaacataa
    80341 cggctggtta ctggaaggac agattgagcg caatcaacag gcgctggaaa tgttgaaacc
    80401 gcatcaggaa ccgacgctat atggggcgaa cggtcagacc tcaacacccc atcgcggcag
    80461 taaaaagatt tcgatctgaa tattatctcc ggcctgcact gcaggccgga atgcattgtt
    80521 acaccgtccg gcgggcaaat tctttaactt tgaagcccag taccgccagt gcagcgaagt
    80581 acgcggcaat ccccgccagc acgaccgcca ttaaacgcag taagcgccag ggcatggtac
    80641 ccaatgacca ctccggcatg atatgtaaca tacctaaaag cacgccagac atcaccagta
    80701 ccgccaccac cagacgcaac agaaacgcca tccagccggg ttgcggagta aagattttct
    80761 gcttacgcaa ctgccagtaa agcagcgaag cattcagaca cgccgccaga ccaatagaaa
    80821 gtgacagccc ggcatgtttc aacggaccaa taaacgccag gttcatcaat tgcgttaaaa
    80881 tcagcgtaac gatggcaatt ttcactggcg ttttaatgtc ctggcgggaa taaaagccag
    80941 gagccaacac tttcactaca atcaggccaa tcaaacccac cgagtaggca attaacgccc
    81001 gctgggtcat cagcgcatca aacgcggtaa atttaccgta ctggaacagc gaaacggtta
    81061 acggacctga aagaatgccc aacgcaaccg cactcggcag tgccaacagg aaacaaagac
    81121 gcaaccccca gtccatcaaa cggttgtatt catcatgatt gccactggca aaacttttcg
    81181 acagcgacgg cagcaaaatg gtgccaagcg ccacgcccag cacaccggag ggaaactcca
    81241 ttaagcggtc ggcgtaatac atccaagaca ccgaaccgga agcaagaaac gaggcaaaaa
    81301 tggtgttgat gattaaggag atctggctca cagagacgcc aaggatcgcc ggtcccatct
    81361 gtttcaccac gcgcattgct ccagcatcgt ggaagttaat gcgcggcagg accagcatgc
    81421 cgatcttctt caggtgcggt agctgataca ccagctgcag aatgccgccg accgtaacag
    81481 cccacgccag cgccagcacc ggtgggttaa agtacggtgc ggcaaacagc gcgaaaccaa
    81541 tcatgctgat gttaagcagt gttggcgcaa acgccggaat cgagaagcgg ttccacgtat
    81601 tcagaatcgc tcccaccagc gacgccaggg agatcagcaa gatataggga aaggtaatct
    81661 ttagtagctg gctggtcagg gcaaatttgt cagctgtgtc agcgaagcct ggcgcggtca
    81721 ccatgatcac ccacggtgcg gcgagcatgc cagcgaccgt cacaaccgcc agcgcaagtg
    81781 tcagcaggcc agaaacataa gagacaaaga cccgcgtggc gtcttcaccc tgcttacttt
    81841 tatattccgc cagaatcggt acaaatgcct gggaaaatgc cccttcggca aagatacggc
    81901 gtaacaagtt aggaagttta aaagcgacga aaaaggcgtc ggttgccatc cctgcgccaa
    81961 agattctggc gacaattgcg tctcgtgcga agccaagcac acgcgaaaac atggtcatcg
    82021 agctgacggc ggccagcgat tttaataaat tcatcggtgt tctaatccgg acccacgggc
    82081 aaaacgcctg caaggcaggc gctgaaagag gcgaatagtc tacctagatt atggtgaatt
    82141 gctaccgcca gatgttacag ggtttattca ctcatcgcat cgcgccagat cttgtcaacg
    82201 atacgttgcg ccagcactgc ctattcgccg gcggtttgcg gaactgtctg attttacacg
    82261 cattcaatga agtgccgcgc acagccgaca aatccacgtt gctcaagagt gctctgccaa
    82321 ccaggaatcg gtttattcac tacaccctgc ccgcgctcct cacgccattc gcgcatatcc
    82381 gtaatgtcga tgagcgcacc gtcagtcacg gcctgcacgg tttcacgctg acttccggca
    82441 cggcgatgca tacaagtggt gatctgcaaa ggaccggccg aaaaatggtg ctcggcaaac
    82501 agcatttcgc cagcgtcgtt agtcagtagc gtaccgccat ccagagaggc tttgccgccc
    82561 gacaaccaca gcgcggtatc caccacatgc agataatcat ccagcaacgt gaaataaaga
    82621 tcgtgtggcc cgacgctatt gctacgatgt ttatccattc ttagcgaggc tgcggtggcg
    82681 agttgcgttt ttaactcacc gtagagtggt gcgaaacgac ggttaaaacc gaccatcaac
    82741 gtcagttttt tccgcgccgc cagttccacc agcctttcag catcgcgcag attttctgcc
    82801 agcggtttat cgacacagac atgtaccccc gcattgagta acgtactgac cacgtcaaag
    82861 tggctggcgg tgctggagtg cacaaaaacc gcatcacaac tggcggcaag gctggataac
    82921 gaatcggcat aaggaatgcg ccagctgtca caaattggca gggctttggc gcgcgtaggc
    82981 gaccaggctc cttgtaacgt ccagtcagac gctgccgcca gcaccggtaa ccacgctttt
    83041 tgcacaatgc cgcctaatcc cactacgccg atacgtaatt ttttcacttt aatctcccag
    83101 atgggccagc aacgaatcaa gacgttgttt cagttctgcc acttcgattt ccagggcttc
    83161 gacgcgggtc tgtaaatcac cgtcaaccat gttcgacata gccgttaccg ccggctgatc
    83221 ttcaacctca ccactgaaaa gatgcatgta gcggctttcg cgtttacccg gttcgcgggc
    83281 cagacgcacc acaaaaggac catcttcgcg attcgccagt tgttccagcg tcgactccac
    83341 ttccgccata tcgctgaatt catacattcg cgcggcgcgg ctgcgcagtt cacccggcgt
    83401 ctgggcacca cgcaataaca acgtggtgat taacgccact tctgcggcgc tcagtttcag
    83461 atcgccaaat tctgaattac aaaaacgttg ctcatatttg gtgacccgat taccaaaacc
    83521 gctcactgtg cgtagataat gacgtttgac cagattatcc agctgttcct gcacttcgga
    83581 ttcactcaga ttcatgaccg gttcacggtt cgttttctga ttacaggccg tgactacacc
    83641 attgactgag agcgggtatt gctccggcgt ggtcacctgc ttttccagca agcaaccaat
    83701 tacgcgagct tccagggcag taagttgata tttcatgaga ttctccttag cggccgggcg
    83761 tccagtctgg ggtagttaat gccgtcagta cgtgatcgcg ccattgtcca tcaatcaaca
    83821 gatagtcttt cgcatagcct tctttttcaa aacccagtcg cgccagtaaa tcaccgctgc
    83881 gtttattgtg cggcatataa ttagccataa tgcgatgaat atgttgggtg cgctgcatat
    83941 aacgaatggc tgcggtcagg gcttcaaaca tgagtccttt gccctgccat ttttgcccaa
    84001 tcgaataacc gagatagcag gcatgaaaag agccgcgaac aacattggaa aaattggcaa
    84061 cgccaataat ctctttttcg tccgggtcga ataagccaaa gtagaaagct gaaccttgtt
    84121 tatgaaattc gttaatcatc cctagcctgg cctgccagcc tgatggataa cagtggcttt
    84181 cgtcgcgcac tggctcccag ggcttgagga aatggcgatt ctctgcgtaa taatccgcca
    84241 gacgccaggc atcacgatca tgcaccagac gcacgaccag tcggtctgtg gttaagcgca
    84301 cttttggcac gttactgcga tagccaaaca ttctatacct actccttccc gtaacgtctt
    84361 taatacgctg taagtattac tatacctgcg caaccagacc ctgtgaaaag tccaaaatgc
    84421 cattttctgc attattcaca tatttgataa aaactctcta tcaaagcgac gttttgataa
    84481 aaaaatactg tccccgaagt gatgggaaaa gtcttagcgg caatgcagaa tagaaagtgc
    84541 tcatctttta tttcccggga ggggaaatgt cccgcgtatc gcaggcgagg aacctgggta
    84601 aatatttcct gctcatcgat aatatgctgg tcgtgctggg gttctttgtt gtcttcccgc
    84661 tgatctctat ccgcttcgtt gatcaaatgg gctgggccgc cgtcatggtc ggtattgctc
    84721 tcggtctacg ccagtttatt cagcaaggtc tgggtatttt cggcggcgca attgccgacc
    84781 gctttggtgc caaaccgatg attgttaccg gtatgctgat gcgcgccgcc ggattcgcca
    84841 caatgggtat cgcccacgaa ccgtggctat tgtggttttc atgcctgctc tcgggactcg
    84901 gcggcacgtt gtttgatccg ccgcgttcgg cgctggtggt gaaactaatc cgtccacaac
    84961 aacgtggtcg ttttttctcg ctgttgatga tgcaggacag tgccagtgcg gtcattggcg
    85021 cattgttggg gagctggctg ttgcaatacg actttcgcct ggtctgcgcc acaggggccg
    85081 ttttgtttgt gctatgtgcg gcgttcaatg cgtggttgtt accggcatgg aaactctcca
    85141 ccgtacgcac gcccgttcgc gaaggcatga cccgcgtgat gcgtgacaag cgttttgtca
    85201 cctatgtcct gacgctggcg ggttactaca tgctggctgt acaagtgatg ctgatgctgc
    85261 caattatggt caacgacgtg gctggcgcgc cctctgccgt taaatggatg tatgccattg
    85321 aagcgtgtct gtcgttaacg ttgctctacc ctatcgcccg ctggagtgaa aagcattttc
    85381 gtctggaaca ccggttgatg gctgggctgt tgataatgtc attaagcatg atgccggtgg
    85441 gcatggtcag cggcctacaa caacttttca ccctgatttg tctgttttat atcgggtcga
    85501 tcattgccga gcctgcgcgt gaaaccttaa gtgcttcgct ggcggacgca agagctcgcg
    85561 gcagctatat ggggtttagc cgtctgggtc tggcgattgg cggcgctatt ggttatatcg
    85621 gtggtggctg gctgtttgac ctgggcaaat cggcgcacca gccagagctt ccgtggatga
    85681 tgctgggaat tattggcatc ttcactttcc ttgcgctggg ttggcagttt agtcagaaac
    85741 gcgccgcgcg tcgtttgctg gaacgcgacg cctgatttga tactcccccc tgactcctcc
    85801 atactgaaag tgaactgaca gccaaatgga ggagtcacgt gaagctatac atttacgatc
    85861 actgccctta ctgcctcaaa gcccgcatga ttttcggcct gaaaaatatc cccgtcgaat
    85921 tacatgttct gctcaacgac gacgcagaaa cacccacccg gatggtcggt caaaaacagg
    85981 ttcccattct gcaaaaagat gacagccgct atatgccaga aagcatggat atcgttcact
    86041 atgtcgataa actcgacggc aaaccgttac tgaccggcaa acgttcccct gccattgaag
    86101 agtggctgcg caaggtcaat ggctacgtca acaaactgct gttgccgcgt tttgccaaat
    86161 cggcatttga tgagttttct actcccgccg cgcgcaaata tttcgtcgac aagaaagagg
    86221 ccagcgcggg taattttgcc gacctgctgg cccactctga cggtctgatt aagaatatca
    86281 gcgatgattt gcgtgcgctg gacaaactga tcgtcaaacc gaacgccgtg aatggcgaac
    86341 tttcggaaga tgatattcag ctatttccgc tactgcgtaa tctgacgctg gtagccggaa
    86401 tcaactggcc aagccgcgtt gctaattacc gcgataatat ggcgaaacag acacaaatca
    86461 atttgttatc atcaatggcg atttaatcct gacccgccgc ggcagcctgt attcgcgggt
    86521 tgccgccgtt ttcagttttt tctgctggcg agtttatcgc tggtgacaca tgctgcgttg
    86581 tgtcaggata ttgacgcaga tttcatgtcg aggactacca tgaacaagtt tttatttgcc
    86641 gctgcattga tcgtcagtgg cctgctcgtt ggctgtaatc aactcaccca atacaccatc
    86701 accgaacaag aaattaacca gtcgcttgcg aaacataata atttctcaaa agatatcggt
    86761 ttacccggcg tggctgacgc ccatattgtt ctgacaaact tgaccagcca aattggtcgc
    86821 gaagagccga ataaggttac cctaaccgga gacgccaatc tggacatgaa ctccctgttc
    86881 ggtagtcaga aagcgaccat gaaactgaag ctgaaagcgc tgcccgtgtt tgataaagaa
    86941 aaaggtgcga tcttcctgaa agagatggaa gtggtcgatg cgacggtaca accggaaaaa
    87001 atgcaaacgg tgatgcaaac gttgcttccc tatttgaacc aggcattacg caattacttt
    87061 aaccagcaac ctgcttacgt cctgcgcgaa gatggcagtc agggcgaagc aatggcgaaa
    87121 aaactggcga aaggcattga agtgaagcca ggcgaaattg tcattccgtt tacggattaa
    87181 tgacgagggc gcattcgcgc cctttatttt tcgtgcaaag gaaaacgttt ccgcttatcc
    87241 tttgtgtccg gcaaaaacat cccttcagcc ggagcataga gattaatgac tgcaccatcc
    87301 caggtattaa agatccgccg cccagacgac tggcaccttc acctccgcga tggcgacatg
    87361 ttaaaaactg tcgtgccgta taccagcgaa atttatggac gggctatcgt aatgcccaat
    87421 ctggctccgc ccgtgaccac agttgaggct gccgtggcgt atcgccaacg tattcttgac
    87481 gccgtacctg ccgggcacga tttcacccca ttgatgacct gttatttaac agattcgctg
    87541 gatcctaatg agctggagcg cggatttaat gaagccgtgt tcaccgctgc aaaactttac
    87601 ccggcaaacg caaccactaa ctccagccac ggcgtgacgt caattgacgc aatcatgccg
    87661 gtacttgagc gcatggaaaa aatcggtatg ccgctactgg tgcatggtga agtgacacat
    87721 gcagatatcg acatttttga tcgtgaagcg cgctttatag aaagcgtgat ggaacctctg
    87781 cgccagcgcc tgactgcgct gaaagtcgtt tttgagcaca tcaccaccaa agatgctgcc
    87841 gactatgtcc gtgacggaaa tgaacggctg gctgccacca tcactccgca gcatctgatg
    87901 tttaaccgca accatatgct ggttggtggc gtgcgtccgc acctgtattg tttacccatc
    87961 ctcaaacgca atattcacca acaggcattg cgtgaactgg tcgccagcgg ttttaatcga
    88021 gtattcctcg gtacggattc tgcgccacat gcacgtcatc gcaaagagag cagttgcggc
    88081 tgcgcgggct gcttcaacgc cccaaccgcg ctgggcagtt acgctaccgt ctttgaagag
    88141 atgaatgctc tgcagcactt tgaagcattc tgttctgtaa acggcccgca gttctatggc
    88201 ttgccggtca acgacacatt catcgaactg gtacgtgaag agcaacaggt tgctgaaagc
    88261 atcgcactga ctgatgacac cctggtgcca ttcctcgccg gggaaacggt acgctggtcc
    88321 gttaaacaat aaaaatcgta gcttcctgtt gtcattaggt tattttacct gtataaataa
    88381 ccagtatatt caacaggggg ctattatgcg aattgaagtc accatagcga aaacttctcc
    88441 attgccagct ggggctattg acgccctggc tggcgaactt tcccgccgta ttcagtatgc
    88501 gtttcctgat aatgaaggcc acgtatcggt acgttatgcc gcagcgaata atttatcggt
    88561 tattggcgca acaaaagaag ataaacagcg cattagcgaa attctccagg aaacgtggga
    88621 aagcgccgat gactggtttg tcagcgaata atatgcagtg atttttgttt ttgccggatc
    88681 gccccggctt ttttatttta cttacgaatt ttgagt
//
LOCUS       DAEBXN010000002        72977 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.002, whole genome shotgun sequence.
ACCESSION   DAEBXN010000002 DAEBXN010000000
VERSION     DAEBXN010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 72977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72977
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.002"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="KJL99_000099"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="KJL99_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBD4434990.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="KJL99_000100"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="KJL99_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBD4434991.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRVGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="KJL99_000101"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="KJL99_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HBD4434992.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="KJL99_000102"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="KJL99_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HBD4434993.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="KJL99_000103"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="KJL99_000103"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HBD4434994.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQILNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="KJL99_000104"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="KJL99_000104"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HBD4434995.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLSLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="KJL99_000105"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="KJL99_000105"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HBD4434996.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /locus_tag="KJL99_000106"
     CDS             complement(4598..5320)
                     /locus_tag="KJL99_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBD4434997.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="KJL99_000107"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="KJL99_000107"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBD4434998.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKASFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /locus_tag="KJL99_000108"
     CDS             6520..6738
                     /locus_tag="KJL99_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HBD4434999.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVAAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="KJL99_000109"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="KJL99_000109"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBD4435000.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /locus_tag="KJL99_000110"
     CDS             complement(7472..7672)
                     /locus_tag="KJL99_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HBD4435001.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVHKDEQVIGIFHPD"
     gene            complement(7682..9419)
                     /gene="kefB"
                     /locus_tag="KJL99_000111"
                     /pseudo
     CDS             complement(7682..9419)
                     /gene="kefB"
                     /locus_tag="KJL99_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
     gene            complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="KJL99_000112"
     CDS             complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="KJL99_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HBD4435002.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10101..12014
                     /locus_tag="KJL99_000113"
     CDS             10101..12014
                     /locus_tag="KJL99_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBD4435003.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHSKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFLGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12014..13036
                     /locus_tag="KJL99_000114"
     CDS             12014..13036
                     /locus_tag="KJL99_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBD4435004.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13030..13248
                     /locus_tag="KJL99_000115"
     CDS             13030..13248
                     /locus_tag="KJL99_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HBD4435005.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13302..14171
                     /locus_tag="KJL99_000116"
     CDS             13302..14171
                     /locus_tag="KJL99_000116"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HBD4435006.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14226..14630)
                     /locus_tag="KJL99_000117"
     CDS             complement(14226..14630)
                     /locus_tag="KJL99_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HBD4435007.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            14932..15564
                     /gene="crp"
                     /locus_tag="KJL99_000118"
     CDS             14932..15564
                     /gene="crp"
                     /locus_tag="KJL99_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HBD4435008.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15615..17705
                     /locus_tag="KJL99_000119"
     CDS             15615..17705
                     /locus_tag="KJL99_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBD4435009.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKHLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTVLHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPESYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17772..18992)
                     /gene="argD/dapC"
                     /locus_tag="KJL99_000120"
     CDS             complement(17772..18992)
                     /gene="argD/dapC"
                     /locus_tag="KJL99_000120"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HBD4435010.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYRYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19078..19641)
                     /gene="pabA"
                     /locus_tag="KJL99_000121"
     CDS             complement(19078..19641)
                     /gene="pabA"
                     /locus_tag="KJL99_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HBD4435011.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            complement(19673..20275)
                     /locus_tag="KJL99_000122"
     CDS             complement(19673..20275)
                     /locus_tag="KJL99_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HBD4435012.1"
                     /translation="MSDKFGEGRDLYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20265..20432)
                     /locus_tag="KJL99_000123"
     CDS             complement(20265..20432)
                     /locus_tag="KJL99_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HBD4435013.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20537..21118)
                     /gene="ppiA"
                     /locus_tag="KJL99_000124"
                     /gene_synonym="rotA"
     CDS             complement(20537..21118)
                     /gene="ppiA"
                     /locus_tag="KJL99_000124"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HBD4435014.1"
                     /translation="MFKSTLAAMAAVFALSALSALSPAAMAAKGDPHVLLTTSAGNIE
                     LELDKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKN
                     EADNGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMD
                     VADKISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21389..22570
                     /gene="tsgA"
                     /locus_tag="KJL99_000125"
     CDS             21389..22570
                     /gene="tsgA"
                     /locus_tag="KJL99_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HBD4435015.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKRAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPVHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGTIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22832..25375
                     /gene="nirB"
                     /locus_tag="KJL99_000126"
     CDS             22832..25375
                     /gene="nirB"
                     /locus_tag="KJL99_000126"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HBD4435016.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25372..25698
                     /gene="nirD"
                     /locus_tag="KJL99_000127"
     CDS             25372..25698
                     /gene="nirD"
                     /locus_tag="KJL99_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HBD4435017.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDKQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25824..26630
                     /gene="nirC"
                     /locus_tag="KJL99_000128"
     CDS             25824..26630
                     /gene="nirC"
                     /locus_tag="KJL99_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HBD4435018.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26649..28022
                     /gene="cobA"
                     /locus_tag="KJL99_000129"
     CDS             26649..28022
                     /gene="cobA"
                     /locus_tag="KJL99_000129"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HBD4435019.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28269..28436
                     /locus_tag="KJL99_000130"
     CDS             28269..28436
                     /locus_tag="KJL99_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HBD4435020.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            28731..30068
                     /gene="frlA"
                     /locus_tag="KJL99_000131"
                     /pseudo
     CDS             28731..30068
                     /gene="frlA"
                     /locus_tag="KJL99_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
     gene            30089..31111
                     /gene="frlB"
                     /locus_tag="KJL99_000132"
     CDS             30089..31111
                     /gene="frlB"
                     /locus_tag="KJL99_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HBD4435021.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31161..31991
                     /gene="frlC"
                     /locus_tag="KJL99_000133"
     CDS             31161..31991
                     /gene="frlC"
                     /locus_tag="KJL99_000133"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HBD4435022.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAIDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYSTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            31988..32773
                     /gene="frlD"
                     /locus_tag="KJL99_000134"
                     /pseudo
     CDS             31988..32773
                     /gene="frlD"
                     /locus_tag="KJL99_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
     gene            32873..33604
                     /gene="phoB"
                     /locus_tag="KJL99_000135"
     CDS             32873..33604
                     /gene="phoB"
                     /locus_tag="KJL99_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HBD4435023.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKSTK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(33764..34849)
                     /locus_tag="KJL99_000136"
     CDS             complement(33764..34849)
                     /locus_tag="KJL99_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBD4435024.1"
                     /translation="MKTFPLQSLTLAEAQQKQFALVDTICRHFPGSEFLAGGDLGLTP
                     SLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGSQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQLIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(34861..36165)
                     /locus_tag="KJL99_000137"
     CDS             complement(34861..36165)
                     /locus_tag="KJL99_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HBD4435025.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIHPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(36177..36530)
                     /locus_tag="KJL99_000138"
     CDS             complement(36177..36530)
                     /locus_tag="KJL99_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HBD4435026.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(36541..37419)
                     /locus_tag="KJL99_000139"
     CDS             complement(36541..37419)
                     /locus_tag="KJL99_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HBD4435027.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(37416..38642)
                     /locus_tag="KJL99_000140"
     CDS             complement(37416..38642)
                     /locus_tag="KJL99_000140"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBD4435028.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINTLGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLSVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(38643..39806)
                     /locus_tag="KJL99_000141"
     CDS             complement(38643..39806)
                     /locus_tag="KJL99_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HBD4435029.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDIITVFTLDKAREVSAAAVKAGRVQPVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVIAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNVPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIQRGEPEIVGRYDSLGNPLEA"
     gene            complement(39890..40252)
                     /locus_tag="KJL99_000142"
     CDS             complement(39890..40252)
                     /locus_tag="KJL99_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBD4435030.1"
                     /translation="METRLNLLCEAGVIDKDVCKGMMQVVNVLEKECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQELLKEFALEVNPCEE
                     GYFLANLYGLWMAANEEV"
     gene            complement(40269..41174)
                     /locus_tag="KJL99_000143"
     CDS             complement(40269..41174)
                     /locus_tag="KJL99_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4435031.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(41444..42448)
                     /gene="trpS"
                     /locus_tag="KJL99_000144"
     CDS             complement(41444..42448)
                     /gene="trpS"
                     /locus_tag="KJL99_000144"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HBD4435032.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFGYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(42441..43199)
                     /gene="gph"
                     /locus_tag="KJL99_000145"
     CDS             complement(42441..43199)
                     /gene="gph"
                     /locus_tag="KJL99_000145"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HBD4435033.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(43192..43869)
                     /gene="rpe"
                     /locus_tag="KJL99_000146"
     CDS             complement(43192..43869)
                     /gene="rpe"
                     /locus_tag="KJL99_000146"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBD4435034.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(43887..44723)
                     /gene="dam"
                     /locus_tag="KJL99_000147"
     CDS             complement(43887..44723)
                     /gene="dam"
                     /locus_tag="KJL99_000147"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HBD4435035.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMERADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(44830..46122)
                     /gene="damX"
                     /locus_tag="KJL99_000148"
     CDS             complement(44830..46122)
                     /gene="damX"
                     /locus_tag="KJL99_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HBD4435036.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPVRQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGK
                     TAGNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVS
                     GVYASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(46214..47302)
                     /gene="aroB"
                     /locus_tag="KJL99_000149"
     CDS             complement(46214..47302)
                     /gene="aroB"
                     /locus_tag="KJL99_000149"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HBD4435037.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTSET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(47359..47880)
                     /gene="aroK"
                     /locus_tag="KJL99_000150"
     CDS             complement(47359..47880)
                     /gene="aroK"
                     /locus_tag="KJL99_000150"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HBD4435038.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(48281..49519)
                     /gene="hofQ"
                     /locus_tag="KJL99_000151"
     CDS             complement(48281..49519)
                     /gene="hofQ"
                     /locus_tag="KJL99_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HBD4435039.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QESEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(49431..49837)
                     /gene="hofP"
                     /locus_tag="KJL99_000152"
                     /pseudo
     CDS             complement(49431..49837)
                     /gene="hofP"
                     /locus_tag="KJL99_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
     gene            complement(49827..50267)
                     /gene="hofO"
                     /locus_tag="KJL99_000153"
     CDS             complement(49827..50267)
                     /gene="hofO"
                     /locus_tag="KJL99_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HBD4435040.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLEMPHEG"
     gene            complement(50251..50790)
                     /gene="hofN"
                     /locus_tag="KJL99_000154"
     CDS             complement(50251..50790)
                     /gene="hofN"
                     /locus_tag="KJL99_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HBD4435041.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(50790..51569)
                     /gene="hofM"
                     /locus_tag="KJL99_000155"
     CDS             complement(50790..51569)
                     /gene="hofM"
                     /locus_tag="KJL99_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HBD4435042.1"
                     /translation="MTFKIWQIGLHLQQQEAAAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            51689..54241
                     /gene="mrcA"
                     /locus_tag="KJL99_000156"
     CDS             51689..54241
                     /gene="mrcA"
                     /locus_tag="KJL99_000156"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HBD4435043.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVVTLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTMMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(54406..54966)
                     /gene="nudE"
                     /locus_tag="KJL99_000157"
     CDS             complement(54406..54966)
                     /gene="nudE"
                     /locus_tag="KJL99_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HBD4435044.1"
                     /translation="MNKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            55286..57421
                     /gene="igaA"
                     /locus_tag="KJL99_000158"
     CDS             55286..57421
                     /gene="igaA"
                     /locus_tag="KJL99_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HBD4435045.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTEASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            57486..58154
                     /gene="yrfG"
                     /locus_tag="KJL99_000159"
     CDS             57486..58154
                     /gene="yrfG"
                     /locus_tag="KJL99_000159"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HBD4435046.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            58165..58566
                     /gene="hslR"
                     /locus_tag="KJL99_000160"
     CDS             58165..58566
                     /gene="hslR"
                     /locus_tag="KJL99_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HBD4435047.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            58591..59460
                     /gene="hslO"
                     /locus_tag="KJL99_000161"
     CDS             58591..59460
                     /gene="hslO"
                     /locus_tag="KJL99_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HBD4435048.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNTQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADP"
     gene            complement(59604..61355)
                     /locus_tag="KJL99_000162"
     CDS             complement(59604..61355)
                     /locus_tag="KJL99_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HBD4435049.1"
                     /translation="MDNVELSPATRWGMIATGLLQGMIATGLLQGLVCYLLIAWLAGK
                     NHSWIVYGVPATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNP
                     WRAEKAIWDFGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLS
                     NGLTWLVLLLWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALAVILARTQSRLIDS
                     IQKLFTLIATGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAI
                     VRDPQKASLPWTGPLRCLIKTALLVAPLYVFVATWGLWLRVAQYGWTVDRLQGALAEL
                     VLLVWSLGYFVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSH
                     MARYQSGKNTPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQH
                     LQEQVSEKVFAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDG
                     QAERILFAFNDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKA
                     WRDLTVDGETLEINLSK"
     gene            61734..63356
                     /gene="pckA"
                     /locus_tag="KJL99_000163"
     CDS             61734..63356
                     /gene="pckA"
                     /locus_tag="KJL99_000163"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HBD4435050.1"
                     /translation="MRVNNGLPPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(63432..64784)
                     /gene="envZ"
                     /locus_tag="KJL99_000164"
     CDS             complement(63432..64784)
                     /gene="envZ"
                     /locus_tag="KJL99_000164"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HBD4435051.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(64781..65500)
                     /gene="ompR"
                     /locus_tag="KJL99_000165"
     CDS             complement(64781..65500)
                     /gene="ompR"
                     /locus_tag="KJL99_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HBD4435052.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            65728..66204
                     /gene="greB"
                     /locus_tag="KJL99_000166"
     CDS             65728..66204
                     /gene="greB"
                     /locus_tag="KJL99_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HBD4435053.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEANWYVNAIEYVKP"
     gene            66301..68622
                     /locus_tag="KJL99_000167"
     CDS             66301..68622
                     /locus_tag="KJL99_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HBD4435054.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     SVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            69060..69287
                     /gene="feoA"
                     /locus_tag="KJL99_000168"
     CDS             69060..69287
                     /gene="feoA"
                     /locus_tag="KJL99_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HBD4435055.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFDVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            69304..71625
                     /gene="feoB"
                     /locus_tag="KJL99_000169"
     CDS             69304..71625
                     /gene="feoB"
                     /locus_tag="KJL99_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HBD4435056.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDATLARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFNVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGNGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVAIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            71625..71861
                     /gene="feoC"
                     /locus_tag="KJL99_000170"
     CDS             71625..71861
                     /gene="feoC"
                     /locus_tag="KJL99_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HBD4435057.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            72064..72951
                     /gene="rpnA"
                     /locus_tag="KJL99_000171"
     CDS             72064..72951
                     /gene="rpnA"
                     /locus_tag="KJL99_000171"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HBD4435058.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESRSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAASRLAP"
BASE COUNT        17590 a        19774 c        18455 g        17158 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gatgatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt caggccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcattccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccacgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacgatctt taacgccgga
     3121 gcagtcaagc gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcaaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagactca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggccag atcgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcgcttgcc
     4441 ggtgaggtca gttggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4501 ttatggcctt ctgctatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctgaaaat
     4621 caccgctctt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgca tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa agggcgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggcgct ggtttcacat ccagcagctc tacgtcaaac
     5581 actagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagaaagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcactacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagttgaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttctttaag agagttttcc
     6121 atgtaacgac ccagcgaagc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gatgctttgc tgtctgcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaggg caacggccat tgtggtcgcc agtagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcgaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg
     6601 aacgtcacgg tggccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccac aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtggcc atgagccagt tcttcttcgg tcgcttcgcg
     6961 aatcgccaca acttcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccactacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggac cctggtcagt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc actaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gtgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tgatgtccgc
     7561 acttaacaca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgcg
     7621 cctgacaggc cgggcatttt gcgcccgcta taaaacgttt tcggattgcc atccttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct ggcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggtttccc tggcgcgaga aatttgtacg gtatcagcat
     7801 gcatcgggat taactcccgc agcattcgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgtgcctg atgcggatgc atgccaagcg tgaccagcgt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaaa agatcgacct gcgtggcgtc gccgtaataa actttgtagc
     8161 catatttacg catcaggtta acggcgctga tatcccgctc cagcacggta atgcgcattt
     8221 tatttgccat cagcaaacga ccaatcacct gaccaaaacg cccgaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgttaaact
     8341 ggcgggatag ccatttatcc accagcttca tcagcaacgg cgccatctgg tcgccctgga
     8401 ataaacgttg tgaagaagcg gtagaaaaga ggacaaaggc aaactcaccg ccctgactca
     8461 acacgccagc aaactgcatc cgctctgagc tacgcacgcc atacaatcgc gccagcagat
     8521 acagcacgag aattttcacc gccaccagca caaccacgct tatcactacc cacaacagat
     8581 gggtataaag caccccgagg ttgagcgaca tgccgacaga gataaagaac aaaccgagca
     8641 gcaagccttt gaagggatcg atagccgttt ccagttcatg gcgatattca ctttccgcca
     8701 gcagcacgcc cgcaataaat gtaccgagcg ccatcgacag ccccagcgca tccataaaca
     8761 atgcggaacc caacaccagc agcagcgtcg cggcggtgaa cacttcccgc acgccagaag
     8821 ctgcaataaa gcggaatacc ggacgcagta aatagcgccc accaatcagc atgccgacaa
     8881 actccagcac cttcatgccg atcttcatcc agtcgaaatg ttcgtctgcc gaccccgcca
     8941 acaacggcac taacgccagt gctgggatca ctgccagatc ctgaaacagc agaaccgaaa
     9001 acccgagctg gccggattcg ctgcgattca tccctttctc acgcatcaat tgcaacgcca
     9061 ttgcagttga agacatcgca aagccaatgc caccgaccac cgccgcctgc caggcgaaat
     9121 ccgtcagcat caataatccc gccagcaacg ccgcgcttaa cagcacctgt gccgcgccta
     9181 cgccaaaaat cgaacgccgc agttgccaaa gtttggaggg attcaactca aggccgatga
     9241 taaacatcag gaataccacg ccgagttccg aaaagtggag gatctcatcg acgtcgctaa
     9301 taaaccccag cccccacggg ccaattgcaa tccccgccag caaatatccc aacacagcgc
     9361 caatccccag ccgcgaagag aaacagcact cctgcgagta aaaaatcgga accttccatc
     9421 agcggcctcc tggagacagc ggatttgcca accagtcacc gtaggctctg gcgtggctcg
     9481 ccagctcctt tgcgctttgc cgtctcgccc agtaaataat gatgggactt aaccaatgca
     9541 tccggcacat gcccgccgcc agttcaaagg ggcgcagcac atcgctcatc gggtagcgat
     9601 tcagcgcgtc ataacggtaa gcactttccg gctcgccggt ggtaatcacg ttacgccagt
     9661 actttcccgc cagttggttt cctcccggcc cgctggcaaa accacgactt aatacccggt
     9721 ccagccactc tttcagtagc gccgggcagc tataggtata aagaggatgc tgaaagacaa
     9781 tcacctcgtg ctcgcgcagt aatgcctgct cacgggggat atcaataaaa aaatcgggat
     9841 agtgcgcgta aaggtcgtgc acggtaacat tgctgagctg cgtggccggt ttaagcagta
     9901 cccggtttgc caccgagtcc tgagattccg gatgggcata cagcagcaaa actttcgctg
     9961 gctgagacat catccccctc ccggtatggt ttttgtgtat agtcgctgtt ttgggctacc
    10021 attgcgcccg gtgcggcagc tcgcccatac attacattat cataatgata agttaacata
    10081 gtctgaacat acggcgcctt atgattgttt tctcctcgtt acaaattcgt cgcggcgtgc
    10141 gcgtcctgct ggataatgcc accgccacca tcaaccccgg gcagaaagtc ggcctggtgg
    10201 gtaaaaacgg ctgtggtaaa tctaccctgc tggcattgct gaaaaatgaa atcagcgccg
    10261 acggcggcag ctacaccttt ccgggaagct ggcaactggc gtgggtgaat caggaaacgc
    10321 cagcgttacc gcaagcggcg ctggaatatg tcattgacgg cgaccgtgaa tatcgtcaac
    10381 tggaagcgca gctgcacgac gccaacgaac gtaacgacgg gcacgccatt gcgaccattc
    10441 atagcaagct ggatgctatt gacgcatgga gtattcgctc ccgtgccgcc agcctgctgc
    10501 acggcctcgg tttcagcaat gaacaactgg agcgcccggt aagtgatttc tccggtggct
    10561 ggcgtatgcg tcttaacctt gcccaggcgc tgatttgccg ttcagacttg ctgctgctcg
    10621 acgaaccgac taaccacctc gatctcgatg ccgttatctg gctggaaaaa tggttgaaga
    10681 gctatcaggg cacgctgatc ctgatctctc acgaccgcga cttcctcgat ccgatcgttg
    10741 ataaaattat tcatatcgaa caacaaagca tgttcgagta caccggcaac tacagttcgt
    10801 ttgaagtaca gcgcgccacc cgtctggcgc agcaacaagc gatgtacgaa agccagcagg
    10861 aacgcgtggc acatctgcaa agttatatcg atcgtttccg tgccaaagcc accaaagcga
    10921 agcaggccca gagccgcatt aagatgctgg aacgtatgga gctgattgcc cccgcacacg
    10981 tcgacaaccc gttccgcttt agcttccgcg cgccggaaag cctgccaaat ccgttactga
    11041 agatggaaaa ggtcagcgca ggctatggcg atcgcattat tctcgactcg attaaattga
    11101 atctggtccc cggctcgcgc attggtctgt taggccgcaa cggcgcaggt aaatcgacat
    11161 taatcaaact gttagccggt gaacttgcgc cagtcagcgg tgaaattggt ctggcgaaag
    11221 ggatcaagct cggctacttc gcccagcatc aacttgaata cctgcgcgcc gacgaatcac
    11281 ctattcaaca tctggcacgt ttagcgccgc aggagctgga acaaaaactg cgtgactacc
    11341 tcggcggctt tggtttcctg ggcgataaag taaccgaaga aacgcgccgc ttctccggtg
    11401 gggaaaaagc ccgcctggtg ctggcattaa ttgtctggca gcgtccgaat ctgctgctgc
    11461 tcgacgaacc gactaaccac cttgacctcg acatgcgtca ggcactcacc gaagcattaa
    11521 tcgagtttga aggcgcgctg gttgtcgttt cgcacgaccg tcatttgctg cgttccacca
    11581 ctgacgatct ctacctggtt cacgatcgta aagtcgaacc gttcgacggc gatctggaag
    11641 attatcaaca gtggttgagc gacgtacaaa agcaggaaaa ccagaccgac gaagcgccaa
    11701 aagagaacgc gaacagcgcc caggcacgta aagatcagaa gcgtcgggaa gcggagctgc
    11761 gtgcgcaaac ccagccgctg cgtaaagaga ttgcccgtct ggaaaaagag atggagaagc
    11821 tgaacgcgca actggcgcag gcggaagaga aactcggcga cagcgaactg tatgaccaga
    11881 gccgtaaagc ggagttgacc gcctgcctgc aacagcaagc cagcgccaaa tccggcctgg
    11941 aagagtgcga aatggcgtgg ctggaagccc aggagcagct tgagcagatg ctgctggaag
    12001 gccaaagcaa ctgatggcgc agataacgac gaccgatgcc aatgaattta gcagcagtgc
    12061 tgaattcacc cctatgcgcg gctttagcaa ttgtcatcta caaactatgc tgccgcgttt
    12121 gtttcgtcgt caggtgaaat tcaccccgta ctggcagcgg ctggagttgc ccgacggcga
    12181 ttttgtcgat ctcgcgtgga gtgaagaccc cgcacaggcg aaacataaac cgcgtttagt
    12241 ggtgtttcac gggctggaag gcagtctcaa tagcccttac gcccacggtc tggttgaggc
    12301 ggcgcaaaag cgcggctggc tgggcgtggt gatgcatttt cgcggatgca gcggtgaacc
    12361 aaaccgtatg caccgcattt accattcggg cgaaaccgaa gacgccagtt ggtttttacg
    12421 ctggctgcaa cgcgaatttg gtcatgcgcc aacggctgcc gtcggctatt cgctcggcgg
    12481 taatatgctg gcctgtttgc tggcaaagga aggcaatgat ctcccggttg atgcggcggt
    12541 gattgtctct gcgccgttta tgctggaagc ctgtagctat catatggaaa agggcttttc
    12601 ccgcgtttat cagcgttact tgctgaacct gttaaaagcc aatgccgcgc gcaagctggc
    12661 agcctacccc ggaacgctgc cgattaatct cgcgcagtta aaatcggtac gtcgcatccg
    12721 tgaatttgac gatctaatca ccgccagaat tcacggctac gccgacgcta tcgactatta
    12781 tcgtcagtgt agcgccatgc caatgctgaa ccggatcgcc aaaccgacgc tgattattca
    12841 cgccaaagac gatccgttta tggatcatca ggtgatccca aaaccggaaa gtctcccctc
    12901 gcaggtggag tatcaactga ctgaacatgg cggtcatgtt ggctttattg gcggtacatt
    12961 acttcatccg caaatgtggc tggagtcacg cattcctgac tggttaacaa cgtatctgga
    13021 ggcgaaatca tgttgattcc gtggcaagac ctctcccccg aaacgctgga aaatttaatt
    13081 gaaagctttg tgttacgtga aggcaccgat tatggtgaac atgagcgtac acttgaacag
    13141 aaagtcgccg acgtcaaacg ccagctacag tgcggagaag cggtgctggt atggtcggag
    13201 ctgcacgaaa cggtcaatat tatgccgcgc agtcagtttc gcgaataacc tgcaaccgtc
    13261 ggcggacgag ataatagtga tagctaagtc atggagttac tatgtctgcc aaacatccgg
    13321 tcattgcggt aacaggatcc agcggcgcgg ggaccaccac caccagcctc gcgtttcgta
    13381 aaatattcgc gcagttaaat ctgcatgcag ctgaggtgga aggcgacagt tttcaccgtt
    13441 acacccgccc ggaaatggac atggcgatcc gcaaagcgcg cgacgccggg cggcatatca
    13501 gctacttcgg ccccgaagcg aacgacttcg gcctgctgga acaaaccttc attgaatacg
    13561 gtcagagcgg caaagggaaa tctcgcaaat atctgcatac ctacgacgaa gccgtaccct
    13621 ggaatcaggt accgggaaca ttcaccccct ggcaaccttt accagaaccc actgatgtac
    13681 tgttttatga aggtttacac ggcggcgtag tcacgccaca gcataacgtt gcgcagcatg
    13741 tggacttact ggtcggcgta gtgcctatcg ttaaccttga gtggattcaa aaactgatcc
    13801 gcgacaccag cgagcgcggg cactcacgag aagcagtgat ggactcggta gtgcgttcaa
    13861 tggaagacta tatcaactac atcacaccgc agttttcccg cacccatctt aacttccagc
    13921 gcgttcccac cgtcgacact tcaaacccgt tcgcggcaaa aggtatcccg tcgctggatg
    13981 aaagctttgt ggtgatccat tttcgtaatc tggaagggat cgatttccct tggctgctgg
    14041 cgatgttgca aggctcattc atttcccaca tcaatacgtt agtggtacct ggcggcaaaa
    14101 tggggctggc aatggaatta attatgctgc cgctggtgca acgattgatg gaaggaaaga
    14161 aaatcgagta actttgctat tatgccggat aaaatactat ccggcttcac aacgggatag
    14221 ttaagtcacg cggcaaccac ttcatacgag tgagtaatat tcaccgcttt ttccagcatc
    14281 aacgccactg aacaatattt ctcggcagag agatcaaccg cacgcgcaac cgctgcgtct
    14341 ttcaggtcgc gaccggtgac gataaaatgc agattaatgt gcgtaaacag gcgtggtgcc
    14401 tcttcgcggc gttcagaggt caattttact tcacaatcga ccacatcctg acgccctttt
    14461 tgcaggatcg aaaccacatc gatggcactg caaccacccg ccgccatcaa caccatttcc
    14521 atcggacttg gtgctttatc gcctgagttg ccgtccatta aaatctggtg accagaggcg
    14581 gattcgccca gaaaagttaa cccttcgacc cacttcactc gcgcttgcat ttttgctact
    14641 ccactgcgtc aattttcctg acagagtacg cgtactaacc aaatcgcgca acggaaggcg
    14701 acctgggtca tgctgaagcg agacaccagg agacacaaag cgaaagctat gctaaaacag
    14761 tcaggatgct acagtaatac attgatgtac tgcatgtatg caaaggacgt cacattaccg
    14821 tgcagtacag ttgatagccc cttcccaggt agcgggaagc atatttcggc aatccagaga
    14881 cagcggcgtt atctggctct ggagaaagct tataacagag gataaccgcg catggtgctt
    14941 ggcaaaccgc aaacagaccc gactctcgaa tggttcttgt ctcattgcca cattcataag
    15001 tacccatcca agagcacgct tattcaccag ggtgaaaaag cggaaacgct gtactacatc
    15061 gttaaaggct ctgtggcagt gctgatcaaa gacgaagagg gtaaagaaat gatcctctcc
    15121 tatctgaatc agggtgattt tattggcgaa ctgggcctgt ttgaagaggg ccaggaacgt
    15181 agcgcatggg tacgtgcgaa aaccgcctgt gaagtggctg aaatttcgta caaaaaattt
    15241 cgccaattga ttcaggtaaa cccggacatt ctgatgcgtc tgtctgcaca gatggcgcgt
    15301 cgtctgcaag tcacttcaga gaaagtgggc aacctggcgt tcctcgacgt gacgggccgc
    15361 attgcacaga ctctgctgaa cctggcaaaa caaccagatg ctatgactca cccggacggt
    15421 atgcaaatca aaattacccg tcaggaaatc ggtcagattg tcggctgttc tcgtgaaacc
    15481 gtgggacgca ttctgaagat gctggaagat cagaacctga tctccgcaca cggtaaaacc
    15541 atcgtcgttt acggcactcg ttaatcccgt cggagtggcg cgttacctgg tagcgcgcca
    15601 ttttgtttcc cccgatgtgg cgcagactga tttatcaccc cgatatcaac tatgcacttc
    15661 gacaaacgct ggtgctatgt ttgcccgtgg ccgttgggtt aatgcttggc gaattacgat
    15721 tcggtctgct cttctccctc gttcctgcct gttgcaatat tgcgggcctt gatacgcctc
    15781 ataaacgttt tttcaaacac ttaatcattg gtgcgtcgct gtttgccacc tgtagcttac
    15841 tgacacagct gctactggta aaagatgttc ccctgccctt tttgctgacc ggattaacgc
    15901 tggtacttgg cgtcactgct gagctggggc cattacacgc aaaattgctt cctgcatcgc
    15961 tgctcgccgc catttttacc ctcagtctgg cgggatacat gccggtctgg gaaccattgc
    16021 tcatctatgc gttgggcact ctctggtacg gattgtttaa ctggttttgg ttctggatct
    16081 ggcgcgaaca accgctgcgc gagtcactaa gtctgctgta ccgtgaactg gcagattatt
    16141 gtgaagccaa atacagcctg cttacccagc acaccgatcc tgaaaaagcg ctgccaccgc
    16201 tgctggtgcg ccagcaaaaa gcggtcgatt taattactca gtgctatcag caaatgcata
    16261 tgctttccgc gcaaaacaat accgattaca agcggatgct gcgtattttc caggaggcgc
    16321 tggatttgca ggaacatatt tcggtcagtt tgcatcagcc ggaagaggtg caaaaactgg
    16381 tcgagcgtag ccatgcggaa gaagttatcc gctggaatgc gcaaaccgtc gccgctcgcc
    16441 tgcgcgtgct ggctgatgac attctttacc atcgcctgcc aacgcgtttt acgatggaaa
    16501 agcaaattgg cgcactggaa aaaatcgccc gccagcatcc ggataatccg gttgggcaat
    16561 tctgctactg gcatttcagc cgcatcgccc gcgtgctgcg cacccaaaaa ccgctctatg
    16621 cccgtgactt actggccgat aaacagcggc gaatgccgtt gcttccggca ctgaaaagtt
    16681 atctgtcact aaagtctccg gcgctacgca atgccggacg actcagtgtg atgttaagcg
    16741 ttgccagcct gatgggcacc gtgctgcatc tgccgaagtc gtactggatc ctgatgacgg
    16801 tattgctggt gacacaaaat ggctatggcg caacccgtct gaggattgtg aatcgctccg
    16861 tgggaaccgt ggtcgggtta atcattgcgg gcgtagcgct gcactttaaa attcccgaaa
    16921 gttacaccct gacgttgatg ctgattacca ccctcgccag ctacctgata ttgcgcaaaa
    16981 actacggctg ggcaacggtc ggttttacta ttaccgcagt gtataccctg caactgttgt
    17041 ggctgaacgg tgagcagtac atccttccac gtcttatcga taccattatt ggttgtttga
    17101 ttgctttcgg tggtaccgtc tggctgtggc cgcagtggca gagcgggtta ttgcgtaaaa
    17161 acgcccatga tgctttagaa gcctatcagg aagcgattcg cttgattctg agcgaggacc
    17221 cgcaaccaac gccactggcc tggcagagaa tgcgggtaaa tcaggcgcat aacactctgt
    17281 ataactcatt gaatcaggcg atgcaggaac cggcattcaa cagccattat ctggcagata
    17341 tgaaactgtg ggtaacgcac agccagttta ttgttgagca tattaatgcc atgaccacgc
    17401 tggcgcggga acaccgggca ttgccacctg aactggcaca agagtattta cagtcttgtg
    17461 aaatcgccat tcagcgttgt cagcagcgac tggagtatga cgaaccgggt agttctggcg
    17521 atgccaatat catggatgcg ccggagatgc agccgcacga aggcgcggca ggtacgctgg
    17581 agcagcattt acagcgggtt attggccatc tgaacaccat gcacaccatt tcgtcgatgg
    17641 catggcgtca gcgaccgcat cacgggattt ggctgagtcg caagttgcgg gattcgaagg
    17701 cgtaatacag gctaaaccat cggataaggc attcacgccg catccgacat attttgcctg
    17761 atacgtctta tttacgcccc aaccaccttc gccaccgcgt gggcgaaacg ttgcatccct
    17821 tcatcgatat ccgcatcttc caccaccagc gacggtgcta aacgcatcac atccggtccg
    17881 gcattcagca ccattacgcc aacctctgca cccgcataca ggaaatcacg cgcccgacct
    17941 ttgtagtgtg gtttcagctc cgcgccaatt aacagcccca taccgcgaat atcgctaaac
    18001 acatcgtact gctgatcgat cttctgcaaa tggtcaacaa aacgctggcg tttcgcctga
    18061 atgccttcca gcacttcagg ggtattgatg atatcaaacg ccgcgcccgc tactgcacag
    18121 gccagaggat taccgccgta ggtggaaccg tgagaaccag gatgaaacgc agaagcaatt
    18181 tccgccgtgg tcagcatggc gctaatcggg aagccgccgc ctaacgcttt cgcagaagtc
    18241 agaatatccg gcgtcacacc gtagtacctg taagcaaaca aatcgccagt ccgccccatc
    18301 ccgcactgca cttcatcaaa caccaataat gcctgatgtt gatcgcacag ctcgcgcaag
    18361 ccctgcaaaa actctggcgt cgctgccgtc acaccgccct cgccctggat cggctcaacc
    18421 accaccgcac aggtgtgatc atccatcacc gctttcactg catggagatc gttaaaggga
    18481 acgtggatga tgtctgccgg tttcggccca aagccgtcgg aatattttgg ctgcccaccc
    18541 accgaaacgg taaacagcga gcgaccatga aaagcgttat ggaaggcaat aattttggtt
    18601 ttgaacgggc tatgacgcac acaggcgtaa tggcgtgcca gtttaaaggc ggtttcgtta
    18661 gcttccgtgc cggagttcat aaacaccacg cgttcggcaa acgtcgcctc aatcagtttg
    18721 cgcccaagac gcagcgccgg ttcattggtg aaaacgttac tgatatgcca cagagtttcg
    18781 ccctgggttt ttaacgcgtt caccagcgca ggatggcaat ggcccaacgc cgtaactgca
    18841 atgccacccg cgaaatcgac atactccttg ccttgctgat cccagattcg gctgccctga
    18901 ccttttaccg gaataaactc tgccggagca taaatcggca ggatcacttc atcgaaagtc
    18961 gcgcgtgtaa ttgctgtttg ttcaattgcc atctcatgat caccctgtta cgcataaata
    19021 aatgtgaaat tataatcaca aaatatgcat aaaaaatcac tgaatggcaa tcggaaatca
    19081 gagatgcagg aaattagcca gcagttgatg tccttgttcg ctaagaatac tttctggatg
    19141 gaactgcaca ccttccagat cccactggcg atggcgaatc cccataatct cgcgggtttc
    19201 gctccaggcc gtcacgtcaa agcacgctgg taatgagtca ggttccacca ccagcgaatg
    19261 gtagcgtgtc acggtaagtg gatttgccag cccccgaaat acgccctcac cgctatgtgt
    19321 aatcggcgag gttttgccgt gcatgacctt tgcggcgcgc acaactttgc cgccaaatgc
    19381 ctgcgccatt gcctgatgac cgaggcagac gccaagaatc ggcaagcgcc cggcatagtg
    19441 gcgaataacg tcaagggaga tcccggcttc atctggcgta caggggccag gtgagatgac
    19501 aattttttgt ggtttaaggg cgtcgatatc cgccagcgtc aacgcatcgt tgcgcttaac
    19561 cagcacatcc gcccccagtt cacaaaagta ctggtagagg ttccaggtaa aagaatcgta
    19621 gttatctata agcaggatca tggcggctcc ggtacgaaag aaccgcgcta ttttactcag
    19681 attccccggc ttcgcttacc actttgctaa atatcatctg cagtgcggtg agatccccca
    19741 ttgccccact ctgatttgcc tgattccagg cctctttttc gataccctgc cagctcagtt
    19801 gatatccggc atgaatcgcc agttgctcga agaagatccg ctgtgccaga ccacttccca
    19861 cccggaaggg atgcagcacg ttgatttcac aatagtaatg cgccagccgc tcgacgaact
    19921 tcgctttctc caggccaacc agataacctt cttcctccag atcctgcatc agggcattgc
    19981 cctctttttc gatataagca aagtggcaga acggcgtatc gccctgataa atatcaactt
    20041 cacgcagttg ccctgcccag tcgaaaatat cctggtacag ctggcgatgg atagtgcgca
    20101 aatgcggtaa accgcgcacc agcggaccaa gctcaatggt cgcagcacgc agcgccgtca
    20161 tttcgtaagc ggcctgttcc agccgctgct gctggcggat gttcagccgg ttacgcatga
    20221 tatcaaggcc aggataaaga tacagatcgc gcccttcgcc gaatttatcg ctcatagtgc
    20281 ctcctcagct cttcaaggcg cgctaaagcc tctgcggcag taagagtgac taaaggcatc
    20341 tcgacgcctt caagacggcg actggcctgg aaattacgat tacgctgaag ctcccagaga
    20401 cgggactttt gcttatcggt gagtttcttc acttgatgcc tccctgaacg tgtccatttg
    20461 ccacaagtat aagcggcaaa tccgggttac gccgggagag cagaagcgcg ggcaagattg
    20521 cccgcgcgag aaatcattac ggcaggactt tagcggaaag gataactacc ggttttgacg
    20581 gcacattctg gtacggacca acatcatgag tcggcacctg ggaaatctta tcggcaacgt
    20641 ccatgccttt caccacttta ccaaataccg cgtaaccgaa atcacgctga ccatggtcaa
    20701 ggaaggcgtt atcggcaacg ttgataaaga actggctggt ggcgctgtct ttgtcagcgg
    20761 tacgcgccat cgcgatggtg ccacgcgtgt tgcgcaggcc gttatcggct tcatttttga
    20821 ttggcgggtt tggttttttc tgctgcatct gctcggtgaa accgccgccc tgaatcataa
    20881 agccaggaat gacgcggtga aaggtagtgt tgttataaaa accgctgttc acataatcga
    20941 caaagttttg cacagacact ggcgcttttt gtttatccag ctccagttcg atgttaccag
    21001 ctgaggttgt caacaatacg tgcgggtccc ctttcgctgc cattgctgcg ggagaaagag
    21061 cagaaagagc agaaagagcg aaaacagcag ccatcgccgc cagggtcgat ttgaacatga
    21121 gatttcctta acaaacagag cagaaaaaaa gcaagtgaag agattctaaa tagccttcaa
    21181 gaacaaggcc atccctttac ctaattttac gtatctgaaa tatatgtaac ctgacgatca
    21241 ctttttattc cgtgatcaaa atcacctctt aaaatgcaat ttagcaaccg attgcaataa
    21301 aacatttaaa cagatcataa aatcacctaa aatcgcccgc tcgcggcata tcccgctatg
    21361 ccattttatt tttgaacgca ggccaatcat gactaacagc aatcgcatca agctcacatg
    21421 gattagcttt ctctcctacg cactgactgg tgcgttggtt attgtcaccg ggatggtgat
    21481 gggaaatatc gccgattatt tcaatctgcc tgtttccagt atgagtaaca ccttcacctt
    21541 cctcaacgcc ggcattttaa tctctatctt cctcaacgcc tggctgatgg aaatcgtccc
    21601 gttgaaaacg cagttacgtt ttggctttct cctgatggtg ctggcggttg ccggtttgat
    21661 gttcagccac agcctggcgc tgttctcgac ggcgatgttc attctcgggg tggtcagcgg
    21721 cattactatg tcgattggta cattcctgat aacacaaatg tatgaagggc gtcagcgcgg
    21781 ttcccgcctg ttatttactg actccttctt cagtatggct gggatgattt tcccaatgat
    21841 cgccgcgttt ctactggcgc gcagcattga gtggtactgg gtttatgcct gcatcgggct
    21901 ggtgtatgtc gctattttta ttctgacctt cggctgtgag ttcccggcac tgggcaaacg
    21961 tgcaccaaaa acggatgctc cggtagagaa agaaaagtgg gggatcggcg tactgtttct
    22021 ctctgttgcg gcgctgtgct acatcctcgg tcagttaggt tttatctcct gggtgcctga
    22081 gtatgccaaa ggcctgggca tgagcctgaa cgacgcgggc acgctggtga gtaacttctg
    22141 gatgtcatac atggtcggca tgtgggcgtt cagctttatt cttcgcttct ttgatttgca
    22201 acgcattctg accgtactgg ctggtctggc tgcgattctg atgtacgtct ttaacaccgg
    22261 aacaccagta catatggcgt ggtcaattct cgctctgggc ttcttctcca gcgcgatcta
    22321 taccaccatc atcaccctgg gttcacagca gaccaaagta ccgtcgccaa aactggttaa
    22381 ctttgtcctg acctgcggaa ccatcggtac tatgttgacc tttgtggtta ccggcacgat
    22441 tgttgaacat agcggtccgc aggcggcact gcttacagca aacggtctgt acgctgtcgt
    22501 ctttgtgatg tgcttcctgt taggtttcgt cagccgtcac cgtcagcata acaccctgac
    22561 ctctcattaa ttgctaatgc cggacggcac tatcgtcgtc cggccttttc ctctcttccc
    22621 ccgctacgtg catctatttc tataaacccg ttcattttgt ccattttttg cacaaacatg
    22681 aaatgtcaga caattccgtg acttaagaaa atttatacaa atcagcaata taccccttaa
    22741 ggagtatata aaggtgaatt tgatttacat caataagcgg ggttgctgaa tcgttaaggt
    22801 aggcggtaat agaaaagaaa tcgaggcaaa aatgagcaaa gtcagactcg caattatcgg
    22861 taacggtatg gtcggccatc gctttatcga agatcttctt gataaatctg atgcggccaa
    22921 ctttgatatt accgttttct gtgaagaacc gcgcatcgct tatgaccgcg tacacctctc
    22981 atcttacttc tctcaccaca ccgccgaaga gctgtcgctg gtgcgcgaag gcttctacga
    23041 gaaacacggc atcaaagttc tggtcggcga acgcgctatc accatcaacc gtcaggagaa
    23101 ggtgattcac tccagcgccg gacgtaccgt tttttatgac aagctgatca tggcaaccgg
    23161 ttcctacccg tggatcccac caatcaaagg ttctgatact caggactgct ttgtctatcg
    23221 caccattgaa gacctcaacg ccattgaatc ctgcgcccgt cgcagcaaac gcggtgccgt
    23281 tgttggtggc ggcctgttag gtctggaagc cgctggcgcg ctgaaaaact taggtattga
    23341 aacccacgtt atcgaatttg cccctatgct gatggcggaa cagcttgatc agatgggtgg
    23401 cgagcagctg cgtcgcaaaa tcgaaagtat gggcgtgcgc gttcacacca gcaaaaacac
    23461 ccttgagatt gtgcaggaag gtgtcgaagc gcgtaaaacc atgcgttttg ccgacggcag
    23521 cgaactggaa gtcgacttta tcgtcttctc taccggtatc cgtccgcgcg ataagctggc
    23581 aacccagtgt ggtctggacg ttgctccgcg tggggggatt gtcattaatg attcctgcca
    23641 gacttccgat ccggatatct acgccatcgg tgaatgcgca agctggaaca accgtgtatt
    23701 tggtctggta gcaccaggct acaaaatggc gcaggtcgcc gttgaccata ttctgggtag
    23761 cgaaaacgcc tttgaaggtg ctgaccttag cgccaagctg aaactgctgg gcgtagacgt
    23821 aggtggtatt ggtgatgcgc acggtcgcac gcctggcgca cgtagctatg tttacctcga
    23881 cgaaagtaaa gagatctaca aacgcctgat tgtcagcgaa gacaacaaaa ccctgctcgg
    23941 tgcggtactg gtgggcgata ccagcgacta cggcaacctg ctgcaactgg tgctgaacgc
    24001 tatcgagctg ccggaaaacc cggattctct gattctgccg gcgcactcgg gtagcggtaa
    24061 gccgtctatc ggtgttgata aactgccgga cagcgcgcaa atctgctcct gcttcgacgt
    24121 cactaaaggt gatctgattg ctgccatcaa caaaggctgc cacacagttg cggcgctgaa
    24181 agctgaaacc aaagcgggta ctggctgcgg tggctgtatc ccgctggtca ctcaggtact
    24241 gaacgcggaa ctggcgaaac agggcatcga agttaacaac aacctgtgcg aacactttgc
    24301 ttattcgcgt caggaactgt tccatttgat ccgcgttgaa ggcattaaaa ccttcgaaga
    24361 actgctggcg aaacacggca aaggctacgg ttgtgaagtt tgtaaaccaa ccgtcggttc
    24421 gctgctggcc tcctgctgga acgaatacat tctgaagccg gaacatactc cgctgcagga
    24481 ttctaacgac aacttcctcg ctaacatcca gaaagacggc acctactcgg tgatcccgcg
    24541 ttctccgggc ggtgaaatca ccccggaagg gctgatggca gtgggtcgta tcgcgcgtga
    24601 atttaatctc tacaccaaaa tcaccggctc ccagcgcctg gcgatgtttg gcgcacagaa
    24661 agacgatctg ccggaaatct ggcgtcagct gattgaagct ggcttcgaaa ccggtcatgc
    24721 ctatgcgaaa gcactgcgta tggcgaaaac ctgcgtgggt agcacctggt gccgctacgg
    24781 tgttggcgat agcgtcggcc tcggcgtgga actggaaaac cgctacaaag gcatccgtac
    24841 gccgcacaaa atgaagttcg gtgtctccgg ctgtacccgt gaatgttcag aagctcaggg
    24901 taaagacgtg ggtattatcg ccactgaaaa aggctggaac ctgtatgttt gcggtaacgg
    24961 cggcatgaaa ccgcgtcatg cggatctgct ggcagcggat atcgatcgcg aaacgctgat
    25021 caaatatctc gaccgcttca tgatgttcta catccgtacc gccgacaaac tgacgcgtac
    25081 cgcgccgtgg ttagaaaacc tcgaaggcgg catcgattac ctgaaagcgg tgatcattga
    25141 cgacaaactg gggctgaacg ctcatctgga agaagagatg gcgcgcctgc gtgaagcggt
    25201 agtgtgtgag tggactgaaa cggtcaatac accgtccgcg cagactcgct tcaaacactt
    25261 catcaacagc gacaagcgtg acccgaacgt gcagatggtg ccagagcgcg aacagcaccg
    25321 tccggcaacg ccgtatgaac gtatcccggt aactctggtg gaggacaacg catgagccag
    25381 tggaaagaca tctgcaaaat cgatgacatc ctgcctgaaa ccggcgtctg cgcactgtta
    25441 ggtgacaagc aggtagcgat tttccgcccg tatcacagcg accaggtgtt tgcgatcagc
    25501 aacattgatc cgttcttcga gtccagcgtg ctatcacgcg ggctgattgc ggaacaccag
    25561 ggcgagctgt gggtcgccag cccgctgaaa aaacagcgtt ttcgcttaag tgatggcttg
    25621 tgcatggaag acgaacagtt ttccgtcaaa cattacgaag cacgagtgaa agacggcgtg
    25681 gtgcagctgc gcggttaatg ttttaacggg aggcgcaatg cctccccttt ttgcatggtc
    25741 ctgtaatgac cttcggtata ttgcaggaca ttttttaaac tttttgtttt attttttgtt
    25801 tttatttttt aaaggataat caaatgttta cagacactat taataagtgt gcggctaatg
    25861 ctgcgcgcat tgcacgcctg tcggcaaata acccgctcgg cttttgggtc agctccgcca
    25921 tggcgggcgc atatgtgggt cttgggatca tcctgatttt cacgctcggt aatttgctcg
    25981 acccgtccgt acgtccgctg gtgatgggcg cgacctttgg tatcgcctta acgctggtga
    26041 ttatcgccgg ttctgaactg ttcaccggac acaccatgtt cctcaccttt ggggtaaaag
    26101 cgggcagcat cagccacggg caaatgtggg caatcctgcc gcaaacctgg ctgggtaacc
    26161 tggtcggttc cgtcttcgtt gccatgctct atagctgggg cggcggtagc ctgctgccgg
    26221 tagataccag catcgttcac tccgtcgcgc tggctaaaac cactgcaccg gcaatggtac
    26281 tgttcttcaa aggtgcattg tgtaactggc tggtttgcct ggcaatctgg atggcgctgc
    26341 gcactgaagg ggcggcgaaa tttatcgcta tctggtggtg tctgctggca tttatcgcgt
    26401 ccggctacga gcactctatc gctaacatga cgctgttcgc gctctcctgg ttcggcaacc
    26461 acagcgaagc ctacacgctg gcgggtattg gtcataacct gctgtgggtg acgctgggta
    26521 atactttatc aggtgccgta ttcatgggat tgggttattg gtatgctacg ccgaaagcga
    26581 atcgtccggt tgcggacaaa tttaatcaaa ctgaaacagc tgccggttaa ttactaaggg
    26641 gtttttacgt ggatcatttg cctatatttt gccaattacg cgatcgcgac tgtctgattg
    26701 tcggcggtgg tgatgtcgcg gaacgcaaag caaggttgct gttagacgca ggcgctcgct
    26761 taacggtgaa tgcattagcg tttattccac agttcaccgc atgggcagat gcaggcatgt
    26821 taaccctcgt cgaagggcca tttgatgaaa gccttctcga cacctgctgg ctggcgattg
    26881 cagcgacgga tgatgacacg cttaaccagc gcgtcagcga agccgctgaa gctcgtcgca
    26941 tcttctgtaa cgtagttgat gcgccgaaag ccgccagctt tattatgccg tcgattattg
    27001 accgctcacc gctaatggta gcggtctcct ctggcggcac ctctccggtt ctggcgcgcc
    27061 tgttgcgcga aaaactcgaa tcactgctgc cgttgcatct gggccaggtg gcgaaatatg
    27121 ccgggcaatt acgcgggcga gtgaaacaac agttcgccac gatgagtgag cgtcgccgtt
    27181 tctgggagaa attgttcgtt aacgaccgcc tggcgcagtc gctggcaaac aacgatcaga
    27241 aagccattac tgaaacgacc gaacagttaa tcaacgaacc gctcgaccat cgcggtgaag
    27301 tggtgctggt tggtgcaggt ccgggcgatg ccgggctgct gacgctgaaa ggactgcaac
    27361 aaattcagca ggcagatgtg gtggtctacg accgtctggt ttctgacgat attatgaatc
    27421 tggtacgccg cgatgctgat cgcgttttcg tcggcaaacg cgcgggatac cactgtgtac
    27481 cgcaggaaga gattaaccag atcctgctgc gggaagcgca aaaaggcaaa cgcgtggtgc
    27541 ggctgaaagg tggcgatccg tttatttttg gccgtggtgg cgaagagctg gaaacactgt
    27601 gcaacgcggg tattccgttc tcggtggttc cgggtattac cgcagcttct ggttgctctg
    27661 cctattcggg tattccactc acgcatcgcg attatgccca gagcgtacgc ttaattaccg
    27721 gacacttaaa aaccggtggc gagctggact gggaaaacct ggcggcagaa aaacagacgc
    27781 tggtgttcta tatggggttg aatcaggccg cgactattca gcaaaagctg attgaacacg
    27841 gtatgcctgg cgaaatgccg gtggcaattg tcgaaaacgg aacggcagtc acgcagcgcg
    27901 tgattgacgg tacgctcacg caactcggtg aacttgctca gcaaatgaac agtccatcgc
    27961 taattattat tggtcgggtt gttggcctgc gcgataaact gaactggttc tccaatcatt
    28021 aatttaaccc gggccagaga attctggcct tcttaatgac tctttttata tattcagcaa
    28081 ataaaacata accccttaat aaatattatc tgctaatggg ttatattcac cgttacagcg
    28141 atttacaaaa ttaagtcatt cgctcactga cgccaattaa atattcctgt atcgttctta
    28201 acgagcagtc tgcttatatt catataatca atgaatatta attaatatat aatacatagg
    28261 gaatgtaaat gaacaaattt attaaagttg cactggtagg tgcagtactg gctacgttaa
    28321 ctgcatgtac tggtcatatt gaaaaccgtg ataagaactg ctcttacgac tacctgctgc
    28381 acccggcaat ttctatttct aaaatcattg gcggttgcgg tcctactgca cagtaagtct
    28441 ctgacagaaa tcggctaaca ccggtttaat tgcccgacgc cctctggatt ttccgagggc
    28501 gtatttgttt ctatgtcctt cttgcccccg cccgtaaaaa tattttcact ttaaattcaa
    28561 ttttataact acatccatta gccacagcgc gatcctgtgc gaaattttgt gatcttcctc
    28621 cacatcacat aacataatat gttgttatat tcatcatgca ttgtcatgtt accttttaaa
    28681 tggctgcaaa ctctccccta caacgtattg gacaagaaaa aggtatcgct atgggaagcc
    28741 aggaactcca acgcaagctc ggattttggg ccgttcttgc aatcgccgtc gggacaaccg
    28801 tcggctccgg tatttttgta tctgtgggtg aagtggcaaa agcagcgggc acgccgtggc
    28861 ttacggtgct cgcgtttgtc attggcgggt taattgtgat cccgcaaatg tgcgtctatg
    28921 cggaactatc caccgcttat ccggaaaatg gcgcagatta tgtttatctg aaaaatgccg
    28981 gaagccgacc gctggctttc ctctccggct gggccagctt ctgggccaac gatgcgccgt
    29041 cattgtcgat tatggcgctg gcgattgtca gcaatcttgg ctttttaacg cctatcgatc
    29101 cgttgctcgg taaatttatc gccgccggat taattatcgc ctttatgttg ctacacctgc
    29161 gctccgttga aggcggcgca gcgtttcaga cgctaattac catcgccaaa attatcccgt
    29221 tcactatcgt cattggcctt gggatcttct ggtttaaagc ggagaatttt gccgccctta
    29281 ccaccactgc gattggcgca acgggcagct ttatggcgct gctggcgggg atctctgcca
    29341 ccagttggtc gtataccggc atggcctcta tctgttatat gaccggcgaa attaaaaacc
    29401 ccggaaaaac catgccacga gcgctgattg gttcctgtct gctggttctg gtgctctaca
    29461 ccctgctggc gctggtgatt tccggcctga tgcccttcga caaactcgcc aattctgaaa
    29521 cgccgatttc cgacgccctg acctagatcc ccgcactcgg cagcaccgct gggctctttg
    29581 ttgccatcac ggcgatgatc gtcattcttg gttcgctttc cagctgcgtg atgtaccagc
    29641 cgcggctgga atacgcgatg gcgaaagaca acctgttctt taaatgcttc ggccatgtgc
    29701 atccgaaata caacacgccg gatgtctcca tcatcctgca aggggcgctg gggatcttct
    29761 tcatcttcgt ttccgatctc accagcctgc tgggttattt caccctggtg atgtgtttca
    29821 aaaataccct caccttcggc tccatcatct ggtgtcgtaa acgcgacgat tacaaaccgc
    29881 tgtggcgtac tccggctttc gggctgatga ccaccctcgc cattgcgtca agcctcattc
    29941 tggtcgcctc aacctttgtc tgggcaccga ttcccggcct tatctgcgcc gccatcgtta
    30001 ttgctactgg tctgcctgct tacgccttct gggcgaagcg tagccgccag ctcaacgctt
    30061 tgtcgtaatg ttatctgaag aaaataaaat gttggatatt gataaaagca ccgtggactt
    30121 tctggtcacc gaaaatatgg ttcaggaagt ggaaaaagtt ctcagccatg acgttccgct
    30181 ggtgcacgcc atcgtggaag agatggtgaa gcgcgacatt gatcgtattt atttcgttgc
    30241 ctgcggctcg ccactcaacg cggcgcaaac ggcgaaacat ctggcggatc gcttttccga
    30301 tcttcaggtc tacgccattt ccggctggga gttctgcgat aacaccccgt atcgcctcga
    30361 cgatcgttgc gcggtgattg gcgtttctga ctacggtaaa acggaagagg taatcaaagc
    30421 gctggagctg ggccgggcct gcggagcact cactgcggcg ttcaccaaac gcgcggacag
    30481 cccgattacc tcggcggcgg aatttagcat tgattatcag gccgactgta tctgggaaat
    30541 tcacctgctg ctctgctaca gcgtggtgct ggagatgatc acccgcctcg cgccgaacgc
    30601 ggaaatcggc aagatcaaaa acgatctcaa gcagttgccg aatgcgctcg gtcatctggt
    30661 acgcacctgg gaagaaaaag gccgccagct tggtgaactg gccagccagt ggccgatgat
    30721 ttataccgtt gctgcgggtc cgctgcgtcc gctgggttac aaagaaggca ttgtaacgct
    30781 gatggaattt acctggacgc acggctgcgt gattgagagc ggagagttcc gccatggccc
    30841 gctggagatt gtcgaaccgg gcgttccgtt cctgttcctg ctcggcaatg atgaaagtcg
    30901 ccacaccacc gaacgcgcca ttaactttgt taaacagcgt actgacaacg tgatcgtcat
    30961 cgattacgcc gaaatttcgc aagggctgca cccgtggctg gcaccgttcc tgatgttcgt
    31021 gccaatggag tggctctgct actacctgtc tatttacaaa gatcacaacc cggatgaacg
    31081 ccgctattac ggtggtctgg tggaatatta atccctctcc cggcccggca acgggccgga
    31141 tttacgcaag gagttacccg atgaaaacag gtatgtttac ctgcggccac cagcggctgc
    31201 ctattgaaca cgcatttcgt gatgcaagcg agctgggtta cgacggcatc gaaatttggg
    31261 gcggtcgccc gcacgcgttc gcgccggact taaaagcggg cggcatcaaa caaatcaagg
    31321 cgctggcgca gacgtatcag atgccgatta tcggttacac gccagaaacc aacggctatc
    31381 cgtataacat gatgctgggc gatgaacata tgcgtcgtga aagcctggac atgatcaagc
    31441 tggcgataga tatggcaaaa gagatgaacg cgggttatac gctgatttct gcggcccacg
    31501 cgggctatct cacgccgcct aacgttatct gggggcggct ggcagagaac ttaagcgaac
    31561 tgtgtgagta cgcggaaaac atcggcatgg atctgatcct cgaaccgtta acgccgtatg
    31621 aatcgaacgt cgtgtgtaat gctaatgatg tgcttcatgc gctggcgctg gtgccttcgc
    31681 cgcgcctgtt cagcatggtc gacatttgcg cgccgtatgt ccaggcggaa ccggtgatga
    31741 gttatttcga caaactgggc gataaattac gtcatctgca tattgtcgac agcgacgggg
    31801 ccagcgacac gcattacatt cctggagaag gcaaaatgcc gctgcgggaa ctgatgcgcg
    31861 atattattga tcggggctat gagggttact ctacggtgga gctggtgacg atgtatatga
    31921 acgagcccag actctatgcc cgccaggcgc tggaacgctt tcgcgcgctg ctgccggagg
    31981 atgagagatg aaaaccctgg cgacaatcgg cgataactgc gtcgatatct acccgcaact
    32041 gaataaagcg ttttctggcg gtaatgcggt caatgtggcg gtgtactgca ctcgctacgg
    32101 catacagccg ggatgcatta cctgggtggg tgacgatgac tacggcacaa agctgaagca
    32161 ggatctcgcc cgcatgggcg tcgatatcag ccatgtccat acgaaacacg gcgttaccgc
    32221 acaaactcag gtggaactgc acgacaatga tcgcgttttt ggcgactaca ccgaaggcgt
    32281 gatggccgac tttgccctga gtgaagagga ttacgcctgg ctggcgcagt atgacattgt
    32341 gcacgcggca atctggggac atgcggaaga cgcattccca cagctgcacg ctgcgggcaa
    32401 acttaccgct ttcgacttct ccgacaagtg ggacagcccg ctctggcaga cactggtgcc
    32461 gcatctcgat tttgcctttg cctccgcacc gcaagaagac gaagcgctgc gtctgaagat
    32521 gaaagcgatt gttgcccgtg gcgcaggaac agtgattgtc acgctgggtg aaaacggcag
    32581 cattgcctag gatggcgcgc agttctggcg tcaggctcct gaaccggtga cggttatcga
    32641 caccatgggt gccggagatt cgttcattgc cggattcctt tgcggctggt ctgcggggat
    32701 gacattaccg caggcgatgg cgcagggaac ggcgtgcgcg gcgaaaacca ttcagtacca
    32761 cggtgcctgg taggtataac gttggcgtga gcatcttcac gccaacgtgc tgttacttgc
    32821 cggaaaacga ccctataatc cgagtaattc attctttatt tcagggtcga ttatgtcagc
    32881 tacggaccgc tactctcatc aactcctcta cgctaccgtc cgccagcggc tgctggatga
    32941 tatcgcgcag ggggtttacc aggccgggca acagatccct accgaaaacg agctttgtac
    33001 acaatataac gtcagccgca ttaccattcg caaagccatc agcgacttag tggcagacgg
    33061 cgtactgatc cgctggcagg gaaaaggcac ctttgtacaa agccagaaag ttgaaaacgc
    33121 cctgcttact gtcagtggtt ttaccgattt tggcgtctca caaggcaagt cgacgaaaga
    33181 gaaagtgatc gaacaggaac gggtcagcgc cgcgccgttt tgcgaaaagc tgaacatccc
    33241 cggaaacagc gaagtgttcc atctctgccg ggtgatgtat ctcgataaag agccgctgtt
    33301 tattgatagt tcatggatcc cgctgtcgcg ttatcctgac tttgatgaga tttacgtcga
    33361 aggaagctcc acctatcagt tatttcagga gcgttttgac acgcgagtgg tcagcgacaa
    33421 aaagaccatc gatatctttg ccgccacccg cccgcaggca aaatggctga aatgcgaact
    33481 gggcgaaccg ttgtttcgca tcagcaaaat cgcctttgat cagaatgaca aaccggtgca
    33541 cgtctccgaa ctcttctgcc gcgccaatcg catcacctta acgattgata ataaaagaca
    33601 ttaaccgtag gccggataag atgcgccagc atcgcatccg gcgatgctgg cgcactgaat
    33661 tttacatccc gcacgttccc ctcaccctaa ccctctcccc aaaggggcga ggggaccgtt
    33721 cagtgcggaa tttagcttga ttgccgaacg gtgctatcca gcattaaata ctcgcaatac
    33781 tctcacgcaa aatccgcagc accgtctctt caccgctgcg attcgggtta atgcgaatcg
    33841 cacaatgttc gagttgtgga ttcgcctggc gaaacgttcc tgaaagtcga tagaagagcg
    33901 gcgggatttc atatttcgac tctgcaccga ccgggtaagg caacgcgcca cgcttttgcg
    33961 cttcttccag caccctggcg gcaatcagct gatgaaactc gacaatcaac accttcgact
    34021 gcgcattggc aatgacggcg cttttcactt ccgccaccgc gccgccgtta agcaaagcca
    34081 gcaaccgttc ggatactccc gcctgcaccg catgcatcac tggcgcgaac accagaccac
    34141 gcaagacttc cagcgcctgt gagccctgga tctggctacc gccggagtaa agcgtggcgc
    34201 gaatccgact aataacatcg gcatcgccaa ccactgcacc aacgccctcc ggcccaaaca
    34261 gcttgaagca ggagaatgtc gagacattcg cgccgcattc acagccgatt cgcgctacct
    34321 tcatcaccgc atagttatca tcggttaacg ctggaacacc tgccgcgcgc aacgttgcca
    34381 gcacatctgc cagcacatag ctgtcctgcg gctgctggcg cgtatgctgc accagcgcgg
    34441 catcaggttg ttgctcgtcg acgacctgct tcagtgccga caggtcattg aaatcagcag
    34501 taataagcgt cagccccatc tgctcaataa taacccgtgt cgtcgggtaa acaggcgcgt
    34561 catgcaccag aagacgctgc cccggtttga gcaaagccgc cagcccggcg cgaatcgcgc
    34621 cagtccccgc gccctgcacc agcgccgcgg cctgtgcgtg aaatgcatca gccagcacct
    34681 gctccacccg ttgggtaata cgcggttgat tcagacttgg agttaaccct aaatcaccgc
    34741 cagcaagaaa ctcgctgcct ggaaaatggc ggcaaatagt gtccaccagc gcaaactgct
    34801 tttgctgcgc ttcagcaaga gtcaggcttt gcaaaggaaa cgtcttcata ggtgccttcc
    34861 ttaagccgga acgaacaggc caagccagta aagaacattc agcagaatac cggtgatcat
    34921 tactgccacg accggtgccg ccattttctg taccggacgg cccagggatt cgttgaggaa
    34981 gtaaatggca accgcgatag agaatccggt ataacccgcc atcttaattg ctgcgaaaat
    35041 cgaaccgacc agcagcgcca cttccatcag catattcatg gcgttacgga tgttatccga
    35101 cgcattacgc accgacgggt agcgtcccag ccatttgccg atcgaacgaa gcagcaagac
    35161 ttccgccgaa ataaccactg cgcctaatac cgctgcaacc atcggattcg gcgagagata
    35221 gcccaccgca taaacaaagg taaagcccgc aactgcatac acacccgttg ctaaggcggt
    35281 ggtggcaatc aacggcacaa accccagtcc gcgcataaat tctgccagag ccgcctgatt
    35341 aatcagcgtt tgcgattgtt ccggcgtcac gcctgcggaa tacgctttct ccagtgtgaa
    35401 gatcgaaact tcgctaccag caaaaatctt catactggca acggcggcaa tcaatgctcc
    35461 cacgatggcg atataaggta agtttttgat aatccgcgac gtgcgctctt caaacaccga
    35521 aagcccgctg gcatcgatgt cattttcatc acgatgacgc aggtcgtgag ttatcgcgat
    35581 ccccagcagc atcaccatgc caataaagat ttcgatggat tcagggttaa gatgtgggaa
    35641 atagcgcacg actaccacac gggtcatcag aacaaccacg gcggcgacca gactttgctt
    35701 ccagccaaac tggtagaaaa tcgccaccag cgggaacagt gcaaaagcag aaaccaccgg
    35761 cgagcttaat tccccgaggc tacctaatac atccaccggc agcgcggtca gcagctggtt
    35821 aactggcaac aggcaagtaa ggatcaacac gccccagata gcgccaagac caaacgccat
    35881 caggctgttt atcgccagta cgccgaggat atcggtagga agaaagagta accatgcgtt
    35941 gagcagcccg gttttcaggg taaaagagat ccccaccgag gccacaaaac caatgctcag
    36001 accaaaagcg atactccccg cctcgcgacg gttcatatag ccttcaatca gttgcggcag
    36061 gatcgggtgg atgccgtcat gaaaaacagc cgccgagcga tgcgccagaa gcgatgtcat
    36121 acccgtcagg cacgccacca cgataatctg aatatacaga tccatagtcg tgccttttat
    36181 ttcagatggt taatcaacat cggaatcgcg tgttcgacgt gctcaacgga aaggccaaac
    36241 gccactttcc cttcagcgat cattttggcg atatgctcgt ctttcgcttt aatgcctggt
    36301 ttggcaatgg tgcaactttt gttatagcca atcaccgcga tggcaatcga caatgccgcg
    36361 cccgcaccgg tattacacgc gccaatgtaa taatctagtt gcccggattt caccttcatt
    36421 gccgcttcca tgtcgttgtg aatgaaaact tcaaaacagc caggagccgt cgcttcaatg
    36481 gtttttttaa tctgctcacg ctgtaagcct gcaacgccaa tctttttcat gagtctgtcc
    36541 ttattggaaa aattgagagg gattttcacg taacatcaca tccacatcgg cctgactgaa
    36601 tcctgactgg cgcaattgcg gaataaaggt ggtcaataaa tagtcatagc cataaccacc
    36661 attggctttt aaatgggagc ggcgcgtaat atccatcgac agcatgacgc ggttcagcaa
    36721 cccacggtca cgtagcgcgt ggagcatcgc aatgcgcttt tcgtccgggt agtaactgtt
    36781 cttaccaatg gtgtcgaact gcacgtacgc gccgagatcg atcatcttca aaatgttgtc
    36841 gaggttgtct ttcagatcgc agtgaccaac ggtgacgcgc gaaagatcaa ctccgtgggc
    36901 ttgcaacaga gccagttgtt ccagccccat ggtgctgaac gacgtatgcg tggagatcgg
    36961 gcgtccggtc tggttatgcg ccagcgcagc ggcaataaat accttctctt ccagcggcgt
    37021 aatctttcct tcgctggagc cgatctccgc gatgatcccg gctttcagct ccgtgccatc
    37081 gataccctgt tcaatttcat cgaccatctc ctgcgccagt tcctgcacgc tgcgggtcac
    37141 cacatgttcc gggaaaaacg catcctggta ataaccagta caggccacca cgttgatccc
    37201 ggtttcgcgc attacatcga gcataaattg cgcattgcgc cccatgtaac ggttggtcat
    37261 ctcaatcaca ttacgtacac cccgggtcat caggtcgttc atctcctggc aaatgaacgc
    37321 atactgatca aggcggcagt ccacgttgtt tttaaagccg gagagatcaa tatgcagatg
    37381 ctcatgggcc agggtgtaac ccgtcggatc aaaactcata gggtgtctcc tgcaaaccgg
    37441 agggaggaaa gaaaagagcg accattttgt ggcggtggcg cacggaaaaa ttcacacacg
    37501 gtagctccca catcagaaag cgtggtgcgc acaccgagct gcgtggcgac cagcccttgc
    37561 tgataaacca gcactggcac cacttcacgg gtatggtggc tgtgaccaat ggtcggatcg
    37621 ttaccgtgat ccgccatcac gaccaggcaa tcatctggct gcatcgcctc aacaagccgg
    37681 gcaaggttac ggtcaacgac ctgcaaacgt tcggcataac gtgcgacgtc ttctgcatga
    37741 ccagcgaggt cggtttcctg gatattgatg caaataaacg ccgtcggatg ggtgttaaat
    37801 tcgtcgaggg tgatatccat aatccgctgg ctatccacca ggttttgcca gctcacacca
    37861 taaggattgc tgacgatatc tgccacctta cccaccagca cggttggcac gcccgcttca
    37921 tacagttttt gcggcacctg cactttttca tcaacaccgt agcccatatg cacgacctgg
    37981 aagccgttgt cataagcgcc agaacgcggc gcattaatac caataaagcg cccttctttg
    38041 ctttctgcgg catcgagaat acgctgactg ttggttaaca ggccgccaaa tgtaatgacc
    38101 cgaccgacct gtacctggtc acgcacgata cgaccaattt tgattgcgtc gtcaaaagag
    38161 atcacagaga gattggcggt aatgttatag acttggccta aatccgcctc gagattatcg
    38221 ccaatcgcaa ccgcctgatt gaccgacaga aattgcagat catcgccacg gcgctccacc
    38281 tgccagccag cggaaactaa tgcctgctca acacggtcaa tcacatcgcg aaaaggcatc
    38341 cgcagcggcg gtaacgggcg cgtgcctaaa atttcctgat gccccataaa ggtatcgcca
    38401 ccttcatgtt gcagctctgc cacgccccag gttgcggaat ctgacggctg catatcgcct
    38461 ggcgcataac ccaatgtgtt gattagcccc agcttctcca gcgttggtag ctgcaaatgc
    38521 ggcaactggc tcaggatgtg accacaggta ttcgccccgg catcttgcgg gcgcaccagc
    38581 gtgacatctt tcattgcccc tacgccaaag ctatcaatca ctaacaccac aaatcgcgcc
    38641 atttatgcct ccagagggtt gccgaggctg tcataacgac caactatttc tggttcgccg
    38701 cgctgaatac cggacaccag caccacatcg ctacgggtga caaaaatctg cgtgcgaaaa
    38761 cagagcacca ctgcactgct caccggatac tcgcctgcca gcggcagggt gtagtcgata
    38821 ctgctgtcat ccacagtttt gagattggtt tcagtaatct tttgattttc tggcgtaaac
    38881 accagcgcat gttgcgcatg accacgacgg tagtaaccgc cgccgtagca gtagctgtcg
    38941 ccacggaaat gatgggagat ttcacttaac cagagcatcg cgatacgttc aggctgatcg
    39001 ccctgctggt ttgccggaat agtaccggtc agcgcatgac cgggttcggc atgagtcgca
    39061 ccgtattccg ccagcaatgg cagcgaagtg cagctggtcg ctgaaggtac gttcagttgc
    39121 tcaatcgcaa taccagattt cgccagttga tcccgtgctt gtaccagcgt gtgaagattc
    39181 ggtgttggca aaactttccc agcagcctca tcccaaagca ggcaagggaa atgggttagt
    39241 ccggctaaat gcagccccgg cagtttctta atttcagcaa tcacctcgtg cagcgaatgc
    39301 tgtacaaaac cgctctcctg ccccggataa agaaaatcat cgtcgctata aactttgagc
    39361 agcacaggct ggacccgtcc ggccttaacc gctgccgcag aaacttcccg cgctttgtcg
    39421 agagtaaaca cggtgatgat gtcggtgccc tgttcaacgg cgtcagcaac ctgatgacaa
    39481 gggatttgta ccagatgccc ctgatgcgcc actggcaaac cagcgcggcg catgactcgc
    39541 gcctctttgt aatccaccgc cacaatgccg ctgtagccta atgccagcaa tttttccgcc
    39601 agccacggat tacgaccaaa ttgcttggtc atcagataca gttcaatgcc gtaaagccgc
    39661 gccgtctcaa tgagccgctt accgttttcc agtatctgat ccacgtcgat cacccagctg
    39721 tccggggcga ttttgccctg ctgccacaaa cttagtgcgg cggaaatcag cgccgggttc
    39781 tggcgtttca atgcttctac aaacatcttg ttgcctcgtt tctgttcctt gaattaaata
    39841 ttcatttttt tgaatattta ggttgtgcga cgtggatttg tcgggagctt caaacctctt
    39901 cgttagcagc catccataat ccataaagat tggccagaaa atagccttct tcacacgggt
    39961 taacttccag agcgaattcc ttcaacaact cctgatgcaa ttgcacaacg gcttgccagt
    40021 ggctggattg cgccagttcc gccagcaact cgttatccag cggctctatt tcttcaccac
    40081 ggcgactgcg catcagtgca ctcgccatat gtgtcatcgc catcgttcct tgctcactgc
    40141 gcaccggcag atggcactct ttttccagta cgttgacgac ctgcatcatg cctttgcaga
    40201 cgtccttatc aataacgcct gcctcgcaaa gcaggttgag tctggtttcc atatcagatg
    40261 cctttcgttt aataactggg ttcctgttcc ccactcacca cccgttgttg atgggcaagc
    40321 agggcgtgtt tatctacaac agcacgcagc aattgttgca ttgggtaatc atcgactcgc
    40381 gtcaggacca cggcttcggt ggcctgtaaa aatcgcggat cgtctgttag cggcgtcgcc
    40441 tctaatccca gcatggtcag ttcgttttcc gccaccacgt tccagatcac cgcatcgaca
    40501 tcgcctttaa caatgcgttg taaactctcg tgataagaga gatcgactcg ttccacatca
    40561 ctatcgccaa aaaagacatc ggtcatgatt ttctgatccg ccgaacgact atcgagacct
    40621 actcgcttaa catttccaga ctcacctttg cggcatatca gttgatgttc accaacatac
    40681 gtatgcgggc caagctcgag ggcgatgcac aaattgtttt ggctgagata actttccgcc
    40741 gccaggcggg aaactactgc catgtcatag acaccgttaa gtaaacactc cacgcgaata
    40801 tccgcgccgc gcatatgcgc atagtaaaaa ggaatgccat caaactgggc tttcaatccg
    40861 ctcgctaaac cttcgtacaa gcgggtatag ggcaaaggca tcgcacatac cacgttgttg
    40921 atatccacat gactcagcaa tgctttgtta tccatctcga caagataact gccattgcgc
    40981 ccacggcgtt caatccgtat cgctccgctt gattccagcg ttttcaacgc ggcctgcgtc
    41041 aggccaaccg atgaacggca ttcagttgcc agctcatcta tggttttcag tcgattaccg
    41101 catttttcac ccaacaaata acgggccagt gtcgtgatga cgacgccttc ttttttgata
    41161 aacgttcgac gcataatata ttttcaataa aatgaatatt tatatcttca ttaaaatgaa
    41221 gataagtggc gacgacgaga tgtggaaacg gcgaggcgct tcacattttt tcgggttgtt
    41281 gcgaaaagag gataaaaaaa ccgggtttcc ccggtttcag agtgatgacg aagtgattta
    41341 ttcgacaatg cgagcaaagg gtaattccca ctggcgcttg cctgctcccc tcaccctaac
    41401 cctctcccca aaggggcgag gggaccgtcc gagcgttttg atattacggc ttcgccacaa
    41461 aaccaatcgc ttcgtacacc gctttcagcg tacgggaagc gtgtacactg gctttttctg
    41521 cgccgtcttt catcacctgt tgcaggaagg cttcatcgtt gcggaaacgg tgatagcgtt
    41581 cctgcaattc agtcagcata ccggaaacgg catcagccac ttcacctttc agatgaccat
    41641 acatcttgcc ttcgaactgt ttttccagtt ctgggatgct ctggcccgtt accgcagaaa
    41701 ggatatccag caggttggaa acgcccgctt tgttctgcac atcgtagcga actaccggcg
    41761 gctcgtcgga gtcagtgacc gcgcgtttga ttttcttcac taccgatttc ggatcttcca
    41821 gcaggccgat aacgttattg cggttatcgt cagacttgga catcttcttg gtcggctcca
    41881 gcagcgacat tacgcgcgcg ccagatttcg gaataaacgg ctccggcacc ttaaagatct
    41941 cgccatacag cgcgttgaaa cgctgggcga tatcgcgact cagttccagg tgctgtttct
    42001 ggtcttcacc caccggtacc agattagttt gatacagcag gatgtccgca gccatcagca
    42061 ccggatagcc aaacagacca gcgttgatgt tctcggcata acgcgcagat ttatctttaa
    42121 actgcgtcat gcgactcagt tcgccgaagt aggtatagca gttcagtgcc cagcctaact
    42181 gcgcatgttc tggtacgtgg gactgaacaa aaatggtgct tttctcagga tcgataccac
    42241 aagccagata caaggccagc gtatccaacg tcgctttacg cagcttctgt gcatcctggc
    42301 gcacggtgat cgcgtgttgg tcaacgatac agtaaatgca atggtagtca tcctgcatgt
    42361 ttacccactg acgcagcgca cccatgtagt taccaatggt caattcacct gagggctgtg
    42421 cgccactaaa aacgatgggc ttagtcattt ttcgattcct gattttcgct atgcggaagc
    42481 cctaatgcgg gcagaaggtc atttatagac tgataaatta catcaggctg gctgagatcg
    42541 atagcctcgc cgtagttgta tccgtaggtt aagccaactg atgggcaacc tgccgctttt
    42601 gccgcctgaa tatcattccg tgagtcgccg acaaacagca tctgttgtgg ggcaattccc
    42661 atccgctcag ccaccagtaa cagcgggtcc ggatgcggtt ttttgttttg cacatcatcg
    42721 ccgccgataa ccacgctaaa gtatttggca atatctaagg cttcgagcag cggcgcgacg
    42781 aacggcgtcg gtttgttggt gaccaagcct agcggcaggc ctttagcctg caacgcgccc
    42841 aacgtatcgg caacgtgcgg gaacaaaaac gtcccctctt cggcaacctc gccatagtag
    42901 cgatcgaaca gtttacgcag aatacgtacc tgttcttctg ccggaatgtc gtcatcaacg
    42961 ggcggtttcc ccattgtttt acgcagagtc gcacgttcct gacgcgccca ggtcaatgcg
    43021 cgctccatca gaacatctgc gccgttacca atccaggtaa taacgcgttc ttcacctgcg
    43081 acgggcaact ccagcgcata cagcgccata tctaccgcag cagcaagacc aggagcactg
    43141 tcgaccagcg taccatcaag atcaaaagcg acgccgcgaa tatcttcaaa cttattcatg
    43201 acttaccttt gccagttcac tgcgcatttc atcaatgact tttttatagt ctggctggtc
    43261 gaagattgcc gaaccggcga cgaacatatc cgcgcccgcc gcagcgattt cgccaatgtt
    43321 gttcaccttc acgccaccgt ccacttccag acgaatgtca aagccagact cgtcgatacg
    43381 gcgacgtact tcgcgcagtt tatccagtgt ttgaggaatg aaagactgac cgccgaaacc
    43441 agggttgacg gacatcagca ggatcacatc cagcttatcc atcacgtaat ccagatagct
    43501 cagaggtgtc gccgggttaa ataccagacc cgctttacag ccattttctt taatcagttg
    43561 cagcgtgcgg tcaacatgct cggaggcttc tggatgaaag gtaatgatgc tggcaccagc
    43621 ggcagcgaaa tcaggcacaa tgcgatcgac gggtttcacc atcagatgta cgtcgatagg
    43681 ggcggtaatg ccgtagttac gcaaggattt cagcaccatt ggcccaatcg tcagattggg
    43741 aacatagtgg ttatccatga cgtcaaaatg cacgacatca gcgccagctg ccagggcttt
    43801 tgcggtatct tcacccaggc gggcaaaatc agccgacaga attgaggggg caatcaaata
    43861 ctgtttcatc cgcttctcct tgagaattat tttttcgcgg gtgaaacgac tcctggtttg
    43921 tacaaagcca gcagttcgtc caccttttta cgtgtgccgc cgttgctgct tatactgcgt
    43981 cgaactttga cgacatgcaa ttttgcgcgc tgataccact cacgcgttaa catcgtatcg
    44041 tgattggaga tcagcactgg aatatggcgc tcaaccagac cttcggcgat ctccgccaga
    44101 tgcgcttgtt gttcaagcgt aaaactgttt gtgtgatacg ccgtaaagtt ggcggtcgca
    44161 gacagcggtg cataaggcgg atcgcaatag acgacggatg catcatctgc gcgctccatg
    44221 ctatcggcgt aagactcaca atagaaaaag gcattctgcg ctttttcagc gaagtgatac
    44281 aactctgctt ccgggaaata gggttttttg tagcggccaa acggcacgtt aaactcaccg
    44341 cgcagattgt aacgacacag gccgttgtaa ccgtagcggt tcaaatataa aaacagtacc
    44401 gcccgacgga acggatcctg gcttttgttg aactcttcgc ggaactgata gtaaacctcg
    44461 gcgcaatttg tttcgggaac aaacagctcg cgtgcggcct gtacgtactc atcagtacgc
    44521 atcttcacaa tgttatagag actgatcagg tcgctattga tatcggcaag gatataacga
    44581 gaaaagtcgg tgttgagaaa caccgacccg gcacctacaa aaggctcaac cagacattcg
    44641 cccttgggca aatgccgttt aatatcatca agcaggggat acttgccccc tgcccacttc
    44701 aaaaaagcgc gatttttctt catgccgatt aactaattac accttctccg gctgtggaga
    44761 aagctccgac agcatccagc gcttcaggca gctcccgcaa cgataaacca ttgcgggaga
    44821 taaccttgat tacttcagat cggcctgtac ctgacgcagc ggtttcgccc acgggttttt
    44881 ggcctgaaca tctgctggca atgtagatac cgcttttttc gcctcttctt tcgaagcgta
    44941 cacgccagaa accaggacat accacggctg accattacgc gtcgtttcat agacaacgta
    45001 gtttttcaga ttctctttct tcgcccaacc gttcaggttg tcgtagttag aggaactgct
    45061 cagctgcaga gtgtaatggc tggacggtgc cgatttcaac gaaccaacat tacctgcggt
    45121 cttccctcca gcggctggtg ttgccgtggt ttgcgccggg gatgccgtct gtactggagc
    45181 cgtagtcgcc gtctcttttg gtgctggcgt agaagtcgca gccggtgcct tcgtgctcgc
    45241 cacaggggca gctggttcag gttcagtacg cttcggcgtc tgtgctaccg gcttcggctc
    45301 cgttttcacg gttgcttgcg gttttttcgg ttcaatcacc gcctgctgac gaactgggcg
    45361 cgtagtggac ggacgttcag cggtttgcgt tttcgccgtg tcacgcgatg cattgccatt
    45421 gcgaacaggc gcaaccgttg cgggttcagt cggcaacgtg gaattgaccg ccacattgtt
    45481 cagctgttgc tgattttgtg gctgggtcag cgcattgttc aggtcaccct gcacttcaac
    45541 acgttgttga ccatccgttg ccaccggggt ttgcccttga gtcggcgtag aagagatcgg
    45601 tggcagagaa acatcctgct gagtattttc cgcagacgtg gttcccggcg ctggctgcac
    45661 gccattcgcc tgatcggtcg cattgccagc aagatcaata ctcttctcgc cagacgcggt
    45721 ttgatcgctg gaagtggtcg agggggcttt tagcgcagaa ccgataccga tgatcaacag
    45781 cagtagaacc agaatgccga cgcccatcat catatactga cgagaagcgg gtttactggc
    45841 tgcttttttg cgcttacgcg gacgacgctc tacgcgctct tcatccacgg tttcatcttc
    45901 ggattcgtca atttcttctt cgatttccgg ttcctcattg cgctcttttt gcgcacgagt
    45961 cggacggcga tcgtcagtgt catcaagttc aatatcatca aaattgatct gcggttcgcc
    46021 acgttcagta cgctcagaac gttcagaaga ttgacgagaa cgaccagtac gacgatcgct
    46081 gggatcgggt ttcagctcgt cttctggttt gaattcatcc atttaacacc ccactaaaag
    46141 gttaatgctt accacgttgc aattaacctg aagctaatag accgcttgat aagcggcctg
    46201 acctttcttg ttgttacgct gattgacaat cggcaatggc gttaagaaca agctcgtgcg
    46261 aaacgccgct gcgaacttca ctcttaccaa ttgccaacgg aagaattaag cgcatctctc
    46321 ccgcaaggac tttcttgtca cgcagcatat gcggtaaata cgcctgcgcg gacatttcgc
    46381 gcggcccatt gaccggtaac ccagcccgcg tgagcagggt tataatacgc tgcgtttcgg
    46441 cagaactaaa ctgcccgaga cgttccgacg tccgcgccgc catcaccata cccgcagcga
    46501 ccgcttcacc atgtaaccaa ttgccatacc ccatttcagc ttcaatggca tgaccaaagg
    46561 tgtgtcccag attcagtaaa gcacgtaacc cggtttcgcg ctcgtcggcg gcgacaactt
    46621 ctgccttcag ttcacaacaa cggcgaatac agtacgccat tgccggaccg tccagacgca
    46681 acaacgcatc cagattctct tccagccagt taaaaaacgc accgtcaaga ataatgccgt
    46741 atttgatgac ttctgccagc cccgacgcta actcacgcgg gggaagcgtt ttcagacagt
    46801 cgagatccac caccactgaa gcaggctggt agaacgcgcc aatcatgttt ttaccgaggg
    46861 gatggttgac cgcagttttg ccgccaacgg aggaatcgac ctgcgacagt aacgtcgtcg
    46921 ggacttgaat gaaacgaaca ccgcgctgat aactcgccgc cgcgaagccg gtcagatcgc
    46981 ccactacgcc gccgccaagc gccaccagcg tagtatcgcg accatgcggc ttttgtaaca
    47041 acgccgtaaa gacggtatcg agtacagcta ggcttttata ctgctcgccg tcagggagga
    47101 taacgctatc gacgttaaca cccgcctgtt caagtacgcc gcggacctta tcgagataca
    47161 gaggagccag ggtttcgctg gtgaccaaca tgacctgctc gcccgatttc agcggtaaga
    47221 atgaagctgg ttcattaaac aaaccagatg cgatggtaat tgggtaacta cgttccccga
    47281 gagtaacgac aatcctctcc ataacgcgac atccacctta attactgtac ccgcagacga
    47341 gtgtatataa agccagaatt agttgctttc cagcatgtga ataatctggt ttgcaaccac
    47401 tttagcgctt tgatcatcag tacgaatggt cacgtcggca atctcttcat acagcggatt
    47461 gcgttcattg gccaacgctt ccagaacttc acgcggcggt gtttcaacgt gcagcaacgg
    47521 gcgtttttta tcacgctgcg tgcgtgcaag ttgcttttcg atggtcgttt caagataaac
    47581 gacaacgcca cgagcggaaa gacggttacg cgtttcacgg gatttcacag agccgccgcc
    47641 agtagccagc acaataccct gtttctcggt caactcattg atgacctttt cttcgcgatc
    47701 gcggaagcct tcttcgcctt ctaaatcgaa aacccagccc acatcagctc cggttcgttt
    47761 ctcaatctct tgatcggaat cgtaaaattc catattgagt tgttgagcta actggcgccc
    47821 aatagtgctt tttccggcac ccataggccc aaccagaaag atattgcgtt tctctgccat
    47881 tttttcggta ctactaagac tattcgttaa tgataaaccc gcttcgctca gagagcgccg
    47941 caggacatga actgaaacct cataagatat tgcgagagtc agactgaaaa ttatctcaat
    48001 actccagcgg gtttggcaac tgaataaatc acccatccct ctgcatatct ggtcgctgca
    48061 agcgcgctgc cttgctacca ccgctctggc gataaatcac cgggtaagat tagcgtaaaa
    48121 aagacagcaa aatgccgcct gaatgataaa tcatcatcat ggaacgcagg cagcgaagag
    48181 caccaactct caaatcggta ctccttgtat gctaaatacc tgcgcgcgtc aaatagatga
    48241 aacacgttca gcataaaaac attaccgctt ttacggctgt ttactcactg gaaaccagtc
    48301 gtggcgtgat aaacaccact aactcgcgtc gttcatcttc ttttccgtca tgacgaaata
    48361 attgcccgaa ccagggaatg tcgccaagca acggtacgct atcctgaccc gatttatttt
    48421 tacgggtaaa aatgccgccc agcgccaacg tttctccgct ttttacctcg acctgcgttt
    48481 cgatctcctg cttatcaatc gccagcactt cgccatcggc ctgctgtagc acctgccccg
    48541 gaacgttctg gctgatgtgt aatttcagcc ggatgcgacc tttttgtaac accgtgggcg
    48601 tgacctccat ccccaggacg gcctctttaa attccaccga cgtcgcgcca ctttccccgc
    48661 tggaaacctg atatggaatt tcgctctcct gtttaatgct ggcaggctga agatgtgagg
    48721 ccagcagacg cggactggcg ataatatcca gctgctgttt ttgttcgagc gcggaaagct
    48781 caagatccag caagcgtccg ttgatgcgcc caatgttaaa accgacatgc gttgtcgccg
    48841 tcgctacgga gaggtcgcta ccaagcgtgg tgacttgccc aacgccacca gcgtgttgcg
    48901 catcggccag cgtccatttc acgcctaact cacgcaaact tttttcatta atggtgacaa
    48961 tatgcgccga cagctcaacc tgcccgaccg gcagatccat ttgcgctacc cactgttcaa
    49021 gcgcgcttaa cgccgttttg ttatctcgta gcaaaaggcg attggtgcgt ttatcgacgg
    49081 tcatactccc tttggcactc agtagcttct cccccgcttt cgccagttct cccgcgtcgg
    49141 cgtattgcag ggttatactg cgattttcca gcggcagatt tgcctgcgcc cgcgcctgct
    49201 ccgcctcctg gcgggcgata ttgtcattct gccaggcaat ggaatgcact gagagaatgt
    49261 tgccttcctg ccgcgttatc agtccggcgc ttttcactac agtttgtagt gcctgcttcc
    49321 agggaacatc tgttagatgt aacgacaccg taccgctgac gtctggcgac acgaccaggt
    49381 tcaacttctc ctgttcagcc agcgcctgca acacctgagc taccggaacg tcatccacca
    49441 tcagcgtcac tttttgcggc tttgccgcct gtacgccggg tatcagcatc aacagtagtg
    49501 cggctatcca ttgcttcatt tgtatctcct tgccgttgcc acaaccattg tggcggttcg
    49561 cagtttgtcc cggtacccag cgttagtacg tctggcgtca gctgtaaaat tgtccagccg
    49621 ttttccagca catcgttttg ctgcacccgt cgccatttct tttgcccgtc ttttattaca
    49681 ccgatgatgc gctcgcctcg ccctaccatc ccctgatagc gccactggct aagttcgcta
    49741 atccggcata tagatcttcc ggcgatttaa aagggtcacg cataccggtt aaaaggcaca
    49801 atgcaatacc tgccaacaac cagcgtttaa ccctcatgcg gcatctccag ttgtagcgtg
    49861 aacaaaagat catcaccttc cacgcttaac gaaaaacggc tcacgctgac gttgcgctct
    49921 gccagccgtg taaatgccga cggcactgct tcccacagcg ttttcaacgc caactcacct
    49981 ccctgcgcgg atggatgcca ggaaaccagt tgcgcgccgg ataactgaaa atccagtggc
    50041 gaaaagggca gcgttttttc ctcgctgaag ggagtggtgt ctaccaggcg atacagtgcg
    50101 gcccactgct ggtgatgact tgcccgtagt cgaattaatg cgtcgcgctc ttcatggtgt
    50161 gtcgatgata gaaaaatgag cgtaactaac atcagcaacc agaatgccca gcaaaactgg
    50221 cggaggcggg gtgatgtggc gaaccaccag tcaaagaaca tgttcatcgc taaccttcct
    50281 tgttaactga tactcaaatt gccagcgtcc ctgcgcatcc tgctgcgtgg ctccccgctg
    50341 attgagatga aaagacgcat cctggcggag tgacgtttct agtgcgttta acgcggtaat
    50401 gcttgttgtc agccccttga tctccagcgt tccctgctgc cagcttatcg ttgtcagcca
    50461 ggcgtgctcg ggtaaaagcg ccgccagtgc ttccagcgca gattgccagt cgcgggtaaa
    50521 ttgtcgctgg cgctgccgtt gcgaacgctg ttcgcgtaac tgctgttgct ccagcaaacg
    50581 tggcttcgtt atctgtaagc tgcgggcgag ttgttgttcc gcctgaagca aaactgcgtc
    50641 tatgcgcgct tcggcgctgc ctgtcagacg cagtattagc gttatcccga cggccagcag
    50701 cagaggcgca acgaacatca gcaaccagaa acgcagaaaa gcggtccggc gttgctgtcg
    50761 ccagggcaaa aaattaattg gcggattcat cagtacgcct tcccaagcgc cagccccagc
    50821 gcgatggcaa agtctccacc aggcggcggt agcggcggct gacgaacaga aacggcctcc
    50881 caggggtcaa atccgccttc gccacatatc gcgacgctgt cgggatcaac ggataacgct
    50941 gccgccagct ccttcgcgct agtcatcccc accgccagtt tgcgccccca gctatagcgc
    51001 gtcgcccaca gccactgttc gttatcacgc caggccagac attgctgatg agaaggtaaa
    51061 aaaggcagga atcgctgtaa tgcactggca tccggggtga tcgcactaac atgaacacgc
    51121 aacctttctg ccagcgtaag cagcgttgcc agctctttgc tttgcgcggc agtcacgtta
    51181 taagcggggc tgagtgagtc ttcgctgtaa tcgaagcgca gggagtccgg atccatatcc
    51241 agctcgcggg ccatcgtccc tgacagccag gccgtttgct cccgctcacc aagggacatc
    51301 gacgggcgcg gaaatgaccg ctgtaatgtg cgactggcgg gaaacgccaa cataatgtga
    51361 tgacgctgcg gcagttcgcg actccacggt aacaacgttt tagccagctg ctgcgcatca
    51421 acaatccgcc catctttgat aatgtcgtgc gccagcggca accgccacca gcgttgcaaa
    51481 aagcattctt ttgcgccccg cacgatcgca accgctgccg cttcttgctg ttgtaaatgc
    51541 aaaccaattt gccagatctt aaatgtcatt gtgatgatct ccttatcacc cgtcactctg
    51601 acgggtatat caatgcgtct ggcttgcctt tatactaccg cgcgtttgtt tataaactgc
    51661 ccaaatgaaa ctaaatggga aatttccagt gaagttcgta aagtattttt tgatccttgc
    51721 agtctgttgc attctgctgg gagcaggctc gatttatggc ctataccgct acatcgagcc
    51781 acaactgccg gatgtggtga cattaaaaga tgttcgcctg caaattccga tgcagattta
    51841 cagcgccgat ggcgagctga ttgctcaata cggtgagaaa cgtcgtattc cggttacgtt
    51901 ggatcaaatc ccaccggaga tggtgaaagc ctttatcgcg acggaagaca gccgcttcta
    51961 cgagcatcac ggcgttgacc cggtggggat cttccgtgcg gcaagtgtgg cgctgttctc
    52021 cggccacgcg tcacaagggg cgagtaccat tacccagcag ctggcgagaa atttcttcct
    52081 cagtccagaa cgcacgatga tgcgtaagat taaggaagtc ttcctcgcga ttcgcattga
    52141 acagttgctg acgaaagacg aaatcctcga gctttatctg aacaagattt accttggtta
    52201 ccgcgcctat ggtgtcggtg ctgcggcaca agtctatttc ggtaaaacgg tcgaccaact
    52261 gacgctgaac gaaatggcgg tgatagccgg gttgccgaaa gcgccttcca cctttaatcc
    52321 gctctactca atggatcgtg ccgtcgcgcg gcgtaacgtc gtactgtcgc ggatgctgga
    52381 tgaagggtat atcacccagc aacagttcga tcagacacgc actgaggcga ttaacgctaa
    52441 ttatcacgcg ccggagattg ctttctctgc gccgtacctg agcgaaatgg tgcgccagga
    52501 gatgtataac cgttatggcg aaagtgccta tgaagacggt tatcgcattt acaccaccat
    52561 cacccgcaaa gtgcagcagg ccgcgcagca ggcggtacgt aataacgtgc tggactacga
    52621 catgcgccac ggctatcgcg gcccggcaaa tgtgctgtgg aaagtgggcg agtcggcgtg
    52681 ggataacaac aagattaccg atacgctgaa ggcgctgccg acctatggtc cgctgctgcc
    52741 tgccgcagtc accagcgcca atcctcaggg agcgacggcg atgctggcgg acgggtcgac
    52801 cgtcgcattg agtatggaag gcgttcgctg ggcgcgtcct taccgttcgg atactcagca
    52861 gggaccgacg ccgcgtaaag tgaccgatgt tctgcaaacg ggtcagcaaa tctgggttcg
    52921 tcaggttggc gatgcatggt ggctggcaca agtgccggaa gtgaactcgg cgctggtgtc
    52981 gatcaatccg caaaatggtg ccgttatggc gctggtcggt ggctttgatt tcaatcagag
    53041 caagtttaac cgcgccaccc aggcactgcg tcaggtgggt tccaacatca aaccgttcct
    53101 ctacaccgcg gcgatggata aaggtctgac gctggcaagt atgttgaacg atgtgccaat
    53161 ttctcgctgg gatgcaggtg ccggttctga ctggcagccg aagaactcac caccgcagta
    53221 tgctggtcca attcgcttac gtcaggggct gggtcagtcg aaaaacgtgg tgatggtacg
    53281 tgcaatgcgg gcgatgggcg tcgactacgc tgcagaatat ctgcaacgct tcggcttccc
    53341 ggcgcaaaac attgtccaca ccgaatcgct ggcgctgggt tcagcgtcct tcaccccaat
    53401 gcaggtggcg cgcggctacg cggtcatggc gaacggcggc ttcctagtgg acccgtggtt
    53461 tatcagcaaa attgaaaacg atcagggcgg cgtgattttc gaagcgaaac cgaaagtagc
    53521 ctgcccggaa tgcgatattc cagtgattta cggtgatacg cagaaatcga acgtgctgga
    53581 aaataacgat gttgaagatg tcgctatctc ccgcgagcag cagaatgttt ctgtaccaat
    53641 gccgcagctg gagcaggcaa atcaggcgtt agtggcgaag actggcgcgc aggagtacgc
    53701 accgcacgtc atcaacactc cgctggcatt cctgattaag agtgctttga acaccaatat
    53761 ctttggtgaa ccaggctggc agggtaccgg ctggcgtgca ggtcgtgatt tgcagcgtcg
    53821 cgatatcggc gggaaaaccg gtaccactaa cagttcgaaa gatgcgtggt tctcgggtta
    53881 cggtccgggc gttgtgacct cggtctggat tggctttgat gatcaccgtc gtaatctcgg
    53941 tcatacaacg gcttccggag cgattaaaga tcagatctca ggttacgaag gcggtgccaa
    54001 gagtgcccag cctgcatggg acgcttatat gaaagccgtt cttgaaggtg tgccggagca
    54061 gccgctgacg ccgcctccgg gtattgtgac ggtgaatatc gatcgcagca ccgggcagtt
    54121 agctaatggt ggcaacagcc gcgaagagta tttcatcgaa ggtacgcagc cgacacaaca
    54181 ggcggtgcac gaggtgggaa cgaccattat cgataatggt gaggcacagg aattgttctg
    54241 attaaaaagg cgcttcggcg ccttttcagt tggctgacaa agtgcatttg tttatgccgg
    54301 atgcgggtga acgcgttatc cggctaacaa aatcgtgaaa aatcaatata ttgcaggaac
    54361 cccataagcc tgataagcgt tgtgcatcag gcaaacttca cgcatttaca ctcgcccctg
    54421 ccctttcaac cattcgcgca cgaggaacag cgcgctgaca ttacgcgctt cgttgaagtc
    54481 agggtcttcc agcaaatcca tcatatgcgc cagcggccag cgcacctgtg gtagcggctc
    54541 tggctcatcg ccttccagcg attccgggta gagatcttgc gcgaccacga tattcatttt
    54601 gctggaaaag taagacggtg ccatgctgag cttcttcaaa aaagtcagat cgttcgcacc
    54661 aaatccaacc tcttctttta gctcgcggtt agcggcttcg tagacgcttt cacccggatc
    54721 aattaatcct ttcgaaaaac ctaattcgta ggattcagtt cccactgcgt attcgcggat
    54781 caggatcagg tgatcgtcca caatcggcac aatcatcact gcttcccggt tggttggacg
    54841 cattcgttca taaacacgcc gcacgccatt gctgaactcc agatccacgc tctcgacggt
    54901 aaacagtcgg gaacgggcta cagtttcaac attcagaatg gtgggttttt gtaatgattt
    54961 gttcatcgtg ggatctatgc tgtgaaatca gcggttattg tgcgatatcg gacacgcttt
    55021 cggcaatgtg aattgcatgt tatttacatt tatgtaactt aataaataat cgtcctcaaa
    55081 tcaaattaaa agtcaatagg ttgaaataac tccaggaatt tgctgatatt ccgccttcag
    55141 agggtttgct atgatcagcg gttactgtga tgtgcttaat gatgctcaag ttaagctcca
    55201 cgcttgccga tagccaaccg cagaatcatg tattgtgtcc ggtgcgactg accacgcctg
    55261 acagactaag taagatgggg aaagcatgag caccattgtg atttttttag ctgctttgct
    55321 ggcctgctca ctacttgcgg gatggctgat aaaagtgcga tccagacggc gtcagctgcc
    55381 ctggaccaac gccttcgcgg atgcgcaaac gcgtaaactc acacctgaag aacgtagcgc
    55441 cgttgaaaat tatcttgaga gcctgacgca ggtattacag gtgcctggcc caacggaagc
    55501 tagcgcggca ccgatctctc tggcgctgaa tgccgaaagc aacaacgtca tgatgctgac
    55561 acacgctatc acacgttacg gcatctctac cgacgatccg aataaatggc gttactacct
    55621 cgattcggta gaagtccacc tgcccccctt ctgggaacag tacatcaacg atgagaatac
    55681 cgttgaactg attcataccg attcgctgcc gttggttatt tcactcaacg gtcatacgct
    55741 gcaggagtac atgcaggaaa cgcgcggcta tgccttgcaa cctgttccgt ccacgcaggc
    55801 gtcgattcgc ggggaagaga gtgagcaaat cgagctactc aatattcgca aagaaacgca
    55861 tgaagaatat gcgctgagtc gtccgcgcgg gctgcgtgaa gcgttgctga tcgtcgcctc
    55921 cttcctgatg ttctttttct gcctgattac cccggatgta tttgttccgt ggctggcagg
    55981 cggcgcatta ctgctgctgg gcgcaggtct gtgggggcta ttcgcgcccc cggcaaaatc
    56041 ctccctgcga gaaattcatt gtctgcgcgg tacaccccgt cgttggggat tgtttggcga
    56101 aaacgatcag gaacagatca acaatatttc actcggtatt atcgacctgg tctatcccgc
    56161 acactggcag ccatacattg ctcaggatct cggtcaacaa accgatattg atatctatct
    56221 cgaccgccac gtagtgcgtc aggggcgata tctttcgctg catgatgaag taaaaaactt
    56281 tccgttacag cactggctgc gcagtacgat tatcgctgcg ggctcgctgc tagtgctgtt
    56341 tatgctgtta ttctggatcc cgctggatat gccgctgaaa ttcactctct catggatgaa
    56401 aggcgcgcag accattgaag ccaccagcgt aaaacaactg gctgacgctg gcgtgcgggt
    56461 aggcgataca ttgcgtatta gcggtacggg aatgtgtaat attcgcactt ccggaacctg
    56521 gagcgcgaaa accaattcac cttttttacc gtttgactgc tcgcagatca tctggaatga
    56581 cgcccgctca ttaccattac cagaatctga actggtcaac aaagcgacgg cattgactga
    56641 agcagttaat cgccagctgc accctaaacc ggaagatgaa tctcgcgtca gtgcctcatt
    56701 acgttcagcg attcaaaaat ccggcatggt attgcttgat gattttggcg acattgtact
    56761 gaagacagcg gatttatgtt ctgccaaaga tgactgtgtg cgactgaaaa atgcgctggt
    56821 caatctcggc aacagtaaag actgggacgc gctggtaaaa cgcgccaacg ccgggaagct
    56881 cgatggcgtg aatgtgttat tacgcccggt gagtgctgaa tcgctggata acctggtggc
    56941 aacctccacc gcgccgttta tcacgcatga aacggcgcga gcggcacaat cactaaacag
    57001 tccggccccc ggcggattcc tgattgtcag cgacgaaggc agcgattttg ttgatcagcc
    57061 ctggccttcg gcatcacttt acgactaccc gccgcaagaa cagtggaacg ctttccagaa
    57121 actggcacaa atgctgatgc atacgccgtt taacgccgaa ggtatcgtca caaaaatctt
    57181 cactgacgcc aatggtacgc agcatattgg ccttcatccg atcccggatc gttccggcct
    57241 gtggcgctat ctcagcacca cattgctgct actgacgatg ctgggtagcg ccatttacaa
    57301 tggcgtacag gcctggcgtc gttaccagcg tcatcgcact cgcatgatgg agattcaggc
    57361 ctattatgaa agctgcctga acccgcaact gatcacccct tcagaaagcc ttatcgaata
    57421 acacgtttgc gcggcaggtt atgctaccct gtcgcgcaaa ttgcttcact ctggagattt
    57481 ccctcatgca tatcaacatt gcctggcagg acgtagatac cgttctgctg gatatggacg
    57541 gcacgttgct cgacctcgcc ttcgataact atttctggca aaagctggtg cctgaaacat
    57601 ggggcgcgaa aaacggggtt acgccacagg aagcgatgga atatatgcgc cagcaatatc
    57661 acgacgtaca gcatacgcta aactggtact gtcttgatta ctggagtgag caactgggtc
    57721 tggatatctg tgcgatgacc accgagatgg gaccgcgcgc cgtactgcgt gaagatacca
    57781 ttccgtttct tgaggcactg aaagccagcg gtaagcagcg aattttgctc accaatgctc
    57841 atccgcacaa cctggcggta aaacttgagc atacgggtct ggacgcacac cttgatttat
    57901 tactttccac ccacacattt ggttatccga aagaggatca gcggttatgg catgcggtgg
    57961 ccgaagctac gggtctgaaa gctgaaagaa cgctgtttat tgatgacagc gaagcgattc
    58021 tcgatgctgc cgcgcaattt ggtattcgtt actgcctcgg cgtgactaat cctgattccg
    58081 ggattgccga gaaacagtat caacgccatc cgtcactgaa tgactaccgc cgcctgatcc
    58141 cctcgctaat gtgaaggaga cgccatgaaa gagaaacctg ctgttgaggt tcgactggat
    58201 aaatggctat gggctgcccg tttttataaa acccgcgcgc tggcccgtga aatgattgaa
    58261 ggcggtaagg tgcattacaa cgggcagcgc agcaagccga gcaaaatcgt cgagctgaat
    58321 gccacgctca ctctgcgcca gggaaatgac gaacgcacgg tgattgtaaa ggcgattact
    58381 gaacagcgtc gccccgccag cgaggcagcc ttgctgtatg aagagactgc ggaaagtgta
    58441 gagaaacgcg aaaaaatggc gatggcacgt aaacttaatg ccttaaccat gccgcacccg
    58501 gaccgacgcc cggacaaaaa agagcgccgc gacctgttac gatttaaaca cggcgacagt
    58561 gaataactgt cacctgcaag agagatgatt atgccgcaac atgaccaatt acatcgctat
    58621 ctgtttgaaa attttgccgt tcgcggcgaa ctggtaaccg tttcggaaac cctgcaacag
    58681 atccttgaga accacgatta tccgcagccc gttaaaaacg tgctggcaga actgctggtt
    58741 gcgaccagcc tgttaaccgc tacgctgaag tttgatggtg atatcaccgt acagctgcag
    58801 ggcgacggtc cgatgaatct ggcggttatt aacggtaaca ataaccagca aatgcgcggt
    58861 gtggcgcgcg tgcagggcga aattccagaa aatgccgacc tgaaaacgct ggtcggcaat
    58921 ggttacgtgg tgatcaccat taccccgagc gaaggcgaac gctatcaggg cgtagttggt
    58981 ctggaaggtg ataccctggc ggcctgccta gaagattact ttatgcgttc tgaacagctg
    59041 ccgacgcgcc tgtttattcg caccggcgac gtagacggca aaccggctgc aggcggtatg
    59101 ttgttgcagg taatgcctgc gcaaaatacc caacaggacg actttgacca cctggcgacg
    59161 ctaaccgaaa ccatcaaaac cgaagaactg ctgaccttac cggcaaacga agtgttgtgg
    59221 cgtttgtatc acgaagaaga ggtgacggtt tacgatccgc aggatgtgga gttcaaatgc
    59281 acctgctcgc gtgaacgttg cgccgatgcg ctgaaaacgc tgcctgatga agaagttgat
    59341 agcatcctgg cggaagatgg cgaaattgac atgcattgtg attactgcgg taaccactat
    59401 ctgttcaatg cgatggatat tgctgaaatc cgcaacaacg cgtctccggc agatccgtaa
    59461 gttcattaat gagtatgtcc ggcagagagg gctctgccgg attttacgac ttaatggcct
    59521 gatgtgctgc gcttaccagc gcctatgaaa taaatcgcaa attattttgg tcagatacag
    59581 cgtcgtatcc gataaaagcg ttgtcactta cttaagttta tttccagcgt ttcaccatct
    59641 accgtcagat cgcgccacgc tttcggcctc gtccccagtt ttccatcctt cgcagctgtg
    59701 agtaattttt ctttcgttat ttcgttcgga agtaaactca tatcaagcgc gtcccattct
    59761 tttctgtctg agtcaaagcc atagacaatg actctgtcat cattaaaagc aaacaggatc
    59821 cgctccgcct gaccatcact attcagatct tgctcgacca gtacgcaggc gtctttttca
    59881 atacaggtca tcacgttata gcgatcctgg attaaggccg accagaatgt cgcatccggt
    59941 ttaacagaac caggggcaat taacacattt tcggcgaata ctttttccga tacctgttcc
    60001 tgaagatgtt gctctccatc taacgccatc agcagatccc gcgcgcgttt cgggtctttc
    60061 ataaacccgg catcgctttt cagcgactca agcgccgcac gtccatagcg accgctctgt
    60121 tcgagcatgt agatagttac ctggtctggc gtgtttttgc cgctctgata acgcgccata
    60181 tgactgttca cgctaatacg catactgtcc agcaccggcg aattaagaag caccagtatc
    60241 accaacacca ataacgaaac cgcaaggttc actttgccct gaagaacaag gggattttgc
    60301 ccattacgcc agacgatact gacaaaatac cccagcgacc acaccagtaa caccagctcc
    60361 gccagcgcgc cctgcaagcg gtcgacagtc cagccgtact gagcgacccg cagccataac
    60421 ccccaggtgg cgacgaacac atacagcgga gcaaccagca aagcggtttt aatcaggcaa
    60481 cgcaacggcc ccgtccaggg aagtgacgct ttttgcggat cgcggacaat agccattaag
    60541 atcaattgca aaaaggccag cgttaacagc aacccggcgg cggagatgtg gcgagaaatc
    60601 gcgctcaggc ccgtaaacgg cagggtgatg ataaacatca gggttagcaa tgatactaac
    60661 ggcagcaacc ccgtggcgat tagcgtgaac aacttttgaa tagagtcgat taaacgtgac
    60721 tgtgtacgcg cgaggatcac cgccagtgcg gtaaccagac caaatgtaag atacataaac
    60781 cagtcggttg caaaaaagag cgtattgaaa aacgtgatgc caacaagttt aaacaactca
    60841 ccccacagta aaagcaccag ccacgtcagg ccattggaga gaaaaatcac cagtaatata
    60901 agtacgttat gccatactga ctggtaaaag tagctatagc ggctactgcc attgcggata
    60961 cgaaggcttt gttgtatcca cggtagcaac aacattgcca tcaacagcag atagcagcca
    61021 aaatcccaaa tcgccttttc agctctccag gggttcatgc catcagtttg ccacttcagc
    61081 caaccgctca tccctgtcgt ggcaataaac accagcgcca gccatcccca aaggcgcttc
    61141 tgtttaaaag agatcacgga gaagagtaaa acggatgaaa acgcgacggt cgcaggcacg
    61201 ccataaacaa tccaactgtg attttttccg gcaagccagg cgataagcag gtagcaaacc
    61261 agcccctgaa gtaatccggt agcaatcatc ccctgaagta atccggtagc aatcatcccc
    61321 cagcgtgttg ccggtgaaag ttcaacgtta tccatgttgg ttatccagaa tcaaaaggtg
    61381 ggttaattat cgcatccggg cagtagtatt ttgctttttt cagaaaataa tcaaaaaaag
    61441 ttagcgtggt gaatcgatac tttaccggtt gaatttgcat caatttcatt caggaatgcg
    61501 attccactca caatattccc gccatataaa ccaagattta accttttgag aacattttcc
    61561 acacctaaaa tgctatttct gcgataatag caaccgtttc gtgacaggaa tcacggagtt
    61621 ttttgtcaaa tatgaatttc tccagatacg taaatctatg agccttgtcg cggttaacac
    61681 ccccaaaaag actttactat tcaggcaata catattggct aaggagcagt gaaatgcgcg
    61741 ttaacaatgg tttgcccccg caagaactcg aggcttatgg tatcagtgac gtacatgata
    61801 tcgtttacaa cccaagctac gacctgctgt atcaggaaga gctcgatccg agcctgacag
    61861 gttatgagcg cggggtgtta actaatctgg gtgccgttgc cgtcgatacc gggatcttca
    61921 ccggtcgttc accaaaagat aagtatatcg tccgtgacga taccactcgc gatactttct
    61981 ggtgggcaga caaaggcaaa ggtaagaacg acaacaaacc tctctctccg gaaacctggc
    62041 agcatctgaa aggcctggtg accaggcagc tttccggcaa acgtctgttc gttgtcgacg
    62101 ctttctgtgg tgcgaacccg gatactcgtc tttccgtccg tttcatcacc gaagtggcct
    62161 ggcaggcgca ttttgtcaaa aacatgttta ttcgcccgag cgatgaagag ctggcaggtt
    62221 tcaaaccaga ctttatcgtt atgaacggcg cgaagtgcac taacccgcag tggaaagaac
    62281 agggtctgaa ctccgaaaac ttcgtggcgt ttaacctgac cgagcgcatg cagctgattg
    62341 gcggcacctg gtacggcggc gaaatgaaga aagggatgtt ctcgatgatg aactacctgc
    62401 tgccgctgaa aggtatcgct tctatgcact gctccgccaa cgttggcgag aaaggcgatg
    62461 ttgcggtgtt cttcggcctt tccggcaccg gtaaaaccac cctttccacc gacccgaaac
    62521 gccgcctgat tggcgatgac gaacacggct gggacgatga cggcgtgttt aatttcgaag
    62581 gcggctgcta cgcaaaaact atcaagctgt cgaaagaagc ggaacctgaa atctacaacg
    62641 ctatccgtcg tgatgcgctg ctggaaaacg tcaccgtgcg tgaagatggc actatcgact
    62701 ttgatgatgg ttcaaaaacc gagaacaccc gcgtttctta tccaatctat cacatcgata
    62761 acatcgttaa gccggtttcc aaagcgggcc acgcgactaa ggttatcttc ctgactgctg
    62821 atgctttcgg cgtgttgccg ccggtttctc gcctgactgc cgatcaaacc cagtatcact
    62881 tcctctctgg cttcaccgcc aaactggccg gtactgagcg tggcatcacc gaaccgacgc
    62941 caaccttctc cgcttgcttc ggcgcggcgt tcctgtcgct gcacccgact cagtacgcag
    63001 aagtgctggt gaaacgtatg caggcggcgg gcgcgcaggc ttatctggtt aacactggct
    63061 ggaacggcac tggcaaacgt atctcgatta aagatacccg cgccattatc gacgccatcc
    63121 tcaacggttc gctggataat gcagaaacct tcactctgcc gatgtttaac ctggcgatcc
    63181 caaccgaact gccgggcgta gacacgaaga ttctcgatcc gcgtaacacc tacgcttctc
    63241 cggaacagtg gcaggaaaaa gccgaaaccc tggcgaaact gtttatcgac aacttcgata
    63301 aatacaccga cacccctgcg ggtgccgcgc tggtagcggc tggtccgaaa ctgtaatgat
    63361 ttgaagctgg agaatatcta tccagtatct tatagaaagc aaaacgggag gcaccttcgc
    63421 ctcccgttta tttacccttc ttttgtcgtg ccctgcgccc gcgttaccgg cactggcagc
    63481 caggcgcgaa tggaaagccc gccccgctcg ctggtgccaa gctccagcat cccgttatgg
    63541 ttatccacga tacgctgcac aattgccagc cctaatcccg tgccgctaat ggtgcgcgca
    63601 ctgtcgccgc ggacaaacgg ctggaacagg tgcttacgtt gttccggcgc aattcccgga
    63661 ccgtcatctt ccacctggaa ccaggcgcga ttcggctccg ttccgctgct gactttgatc
    63721 cagccattgc cataacgggc ggcgttgacc accatattcg ccaccgcgcg tttgatcgac
    63781 agcgggtgca ttttcacttc aatgctgccg gggtaaagcg cggtttcaat ttcccgctca
    63841 tagccacttt cggcagcaat cacctcaccg agcactgcat taagatccgc catttccatc
    63901 ggcatctcct gcccggtgcg caggtagtcg ataaactgct caatgatggc gttgcactct
    63961 tcgatatctt tattgatcga ttctgccaga tagccatcct gctcgctcat catctcagtc
    64021 gccaggcgaa tacgcgtcag cggcgtgcgc aagtcgtgac ttacccccgc catcagcagc
    64081 gtgcggtcat ccgccagttg cttaacacca gccgccatat ggttaaaggc acgggtaacg
    64141 gaacgcacct ccgaagcgcc atattcacgc agcggcggcg gaataatccc tttaccaacc
    64201 tgcaaggctg cgtgttcgag atcgaccaac ggtcggttct ggatacgaat aaacagccac
    64261 gccccgccta tcgccaatag cataatcgcc agcgtatagc ggaacagcgg agagaaatcg
    64321 ccctgatgaa tttcggtcag cggcacgcgt acccagatat tgggcgacag ccaggttttc
    64381 agccagacga caggcgaact tttgttgacc tcaacgcgca cttccgtcgg gccgcccagt
    64441 tgctgtgcca tctgatggct taagaattca tagtgttgcg cccaacgcag acctgcctct
    64501 tcggcagcct cgttggagta gagagagatc cccagctcac ggtagatctc ccgacggaaa
    64561 gcgggaggca caaccaactg cgtgccgtcc tccagttgca gtttgtcggt catcaacata
    64621 cgcacttcgt acgcgaggac tttattaaac tgctggaggc tcggcaaaat cgcgaagttc
    64681 agcaccacca gataagtcgt caccaggctg gcgaacagca aggtgacgat gagcaataac
    64741 gtacgggcaa atgaacttcg tggcgagaag cgcaatcgcc tcatgcttta gagccgtccg
    64801 gtacaaagac gtagcccagg ccccaaacgg tctgaatgta acgcggatgc gctggatctt
    64861 cttctaccat gcggcgcaga cgcgaaatct gcacgtcgat ggagcgttcc attgcggaat
    64921 attcacgacc acgggcaagg ttcatcagct tatcgcggga gagcggctca cgcggatggc
    64981 tgaccagtgc cttcagtacc gcaaactcac cgctggtgag cggcatcggc tcatcttcgc
    65041 ggaacatttc gcgcgtaccg aggttaagtt tgaacttacc gaaagcaatt accgcctctt
    65101 cctgtgacgg tgcgcctggc agttcgttcg cctgacgacg cagcaccgca cgaatacggg
    65161 ccagcagttc acgcgggtta aacggttttg gaatgtagtc gtcagcgcca atctccaggc
    65221 ctacgatacg gtccacttct tcccctttcg ccgtcaccat aatgatcggc atcgggttgc
    65281 tctgactacg aagacgtcgg caaatcgaca agccatcttc accaggtaac attaaatcca
    65341 gtaccataag atggaaagat tcacgagtca gcaggcgatc catctgttct gcattagcga
    65401 cgcttcgaac ttggaagcct tgttcggtga gataacgttc cagcagcgca cgcaggcgca
    65461 tgtcgtcatc gaccaccaga atcttgtagt tctcttgcat tgtttgtact cccaaaggtt
    65521 cgcaacaatt tgtaagcgtg tattcttaaa aaagctcacg ttcgtcacca gctaaatctg
    65581 gtatgaattt cagcctaaat tgttacaaag catattaaac agcagcttaa gtatacaatt
    65641 tattcggcga aacattattg attctgttga tatgatcacg ttatacccaa tgtgcgcatt
    65701 atcaaacaga caaagggaat caacgagatg aaaacgcccc tggttacccg ggaagggtat
    65761 gaaaaactca aacaagagct taattatctc tggcgtgaag aacgcccgga ggtcacaaaa
    65821 aaggtgacct gggccgcaag tctgggcgac cgcagcgaaa atgctgacta tcagtataat
    65881 aaaaagcgtc tgcgtgaaat cgaccgtcgc gtgcgctatc tcactaaatg cctggaaaat
    65941 ctcaaaatcg tcgattactc ccctcagcag gaaggcaaag tcttttttgg cgcgtgggtg
    66001 gagattgaaa acgacgatgg cgtgactcac cgtttccgta ttgtcggcta cgatgaaatt
    66061 tttggccgta aagattacat ctctatcgat tccccgatgg cccgcgcatt gctgaaaaaa
    66121 gaagtcggcg atctggcggt ggtgaatacc cctgccgggg aagcgaactg gtatgttaat
    66181 gctatcgagt acgtgaaacc gtaaggaaga gtcttaacct cctgccgatg gctggcattt
    66241 ttgccagcca gtccgtataa ctatcccctg atttttgatc cgaaaagatg aactcaaacc
    66301 atgatgaatg attcgttctg ccgcattatt gcgggtgaaa ttcaggcgcg cccggaacag
    66361 gttgacgctg ccgttcgcct gcttgacgaa gggaataccg tgccgtttat cgcacgttat
    66421 cgtaaggaaa tcaccggcgg tctggatgac acgcagctgc gtaatctgga aacgcgtctg
    66481 agctatctgc gcgagctgga agagagacgt caggcgatcc tcaaatccat ttccgagcaa
    66541 ggcaaactca ccgatgatct ggcgaaggcc atcaacgcca ccctaagcaa aaccgaactc
    66601 gaagacctct acctgcccta caaacctaaa cgccgcaccc gcgggcaaat cgccattgaa
    66661 gcagggcttg agccgttggc tgacctgctg tggagcgatc cgtcacacac gccagaagtc
    66721 gccgctgcac aatatgttga tgccgataaa ggcgtggcag ataccaaagc cgcgctggat
    66781 ggcgcgcgct atatcctgat ggaacggttt gccgaagatg ccgcgctgct ggcgaaagtg
    66841 cgtgattatc tgtggaagaa cgcgcatttg gtttctacgg tggtgagcgg taaagaagag
    66901 gaaggggcga aattccgcga ctatttcgat catcacgaac cgttgtccac ggtgccttct
    66961 caccgcgcgc tggcgatgtt ccgtgggcgt aacgaaggcg tactccagct ttcgctgaat
    67021 gccgatccgc agttcgatga gccgcccaaa gagagctatt gcgagcaaat catcatggat
    67081 caccttggcc tgcgcctgaa caatgccccg gcggatagct ggcgcaaaag cgtagtgagc
    67141 tggacctggc gcatcaaggt gctgatgcat ctggaaaccg aactgatggg taccgtgcgc
    67201 gaacgcgcgg aagatgaagc aatcaacgtc tttgcccgta acctgcacga tctgctgatg
    67261 gcggcccctg ccggactgcg tgcaacgatg ggcctcgatc cgggtctgcg taccggggta
    67321 aaagtggcgg tggtcgatgc cactggcaaa ctggtggcga ccgataccat ttacccgcac
    67381 accggacaag ccgcaaaagc agcgatgacc gttgctgcct tgtgtgaaaa acataacgtt
    67441 gaactggtgg cgatcggcaa cggtacagct tcccgcgaaa ctgaacgttt ctatctcgac
    67501 gtgcagaagc agttcccgaa agtgaccgca cagaaagtga tcgtcagcga agctggcgcg
    67561 tcggtttact cagcttccga gctggcagcg caggagttcc cggatctcga cgtttcgctg
    67621 cgtggtgcgg tatctatcgc ccgccgtttg caggatccgc tggcggagct ggtgaaaatc
    67681 gatccgaaat ctatcggcgt aggtcagtat cagcatgacg tcagccagac gcaactggcc
    67741 cgcaaactgg atgcagtagt agaagactgc gtaaacgccg ttggcgtcga tctcaacacc
    67801 gcttctgttc cgctattaac ccgcgtggcg ggcctgacgc gcatgatggc gcaaaacatc
    67861 gtggcctggc gcgatgagaa cggccagttc cagaaccgtc agcaactgct gaaagtcagc
    67921 cgtctggggc cgaaagcctt cgagcagtgc gcgggcttcc tgcgcattaa ccacggtgat
    67981 aacccgctgg acgcctctac cgttcatccg gaagcctatc cggtggtgga acgcattctg
    68041 gcagcaacac agcaggcact gaaagatctg atgggtaaca gcagcgaact gcgtaacctg
    68101 aaagcgtctg actttactga tgaaaaattc ggtgtgccga cagtaactga catcatcaaa
    68161 gagctggaaa aaccgggtcg cgatccgcgt ccggaattta aaaccgctca gtttgccgat
    68221 ggcgtcgaga caatgaacga cctgcaaccg ggtatgatcc tcgaaggcgc agtgaccaac
    68281 gtcaccaact ttggcgcgtt tgtcgatatt ggcgtgcatc aggacggcct ggttcacatc
    68341 tcttcattgt cgaacaagtt tgtggaagat ccgcataccg tggtgaaagc gggcgacatt
    68401 gtgaaggtga aagtgctgga agtggatctt cagcgtaaac gtatcgccct gactatgcgt
    68461 ctggacgagc agcctggcga aaccaactct cgtcgcggcg gcggtaatga acgcccgcaa
    68521 aacaaccgcc cggcagccaa accacgcggt cgtgaagcgc agcctgccgg taatagcgcg
    68581 atgatggatg cgctggcggc agcgatgggt aaaaaacgtt aaacgcccgg actggcccct
    68641 aaacctgcgc gataaggcgt tcacgccgca tctggcaacg aacgctttgc ctgacataaa
    68701 agtgccggag aatatctccg gcacttttat tccacagcca aactcacaat atactccgtt
    68761 aatatttatc acttcattaa caactgaaac cttaattaaa cattagccag tccgggtaac
    68821 tcactattcg aattatattt tcgctgcgat ataaccttga gccacatcaa cattgagtca
    68881 gattattatt caaaccaaca ttcgcacaca ttttaagtat tgctgataga aaccattctc
    68941 attatcattg tgttgttgat tatttaatct ctcctttgtt ggcaaatcat ctggtctcat
    69001 gtcgcggtca aacgccccat gaggtagtta tccagttaat gagaaacaag taggcaccta
    69061 tgcaatacac tccagatact gcgtggaaaa tcactggctt ttcccgtgaa atcagcccgg
    69121 catatcgcca aaaactgctt tctctaggca tgttacctgg ctcctctttt gatgtggtgc
    69181 gcgtcgctcc actcggcgac cccattcata tcgaaacccg tcgtgtgagc ctggtattac
    69241 gcaaaaaaga tctggcctta ttagaagtgg aagcggtttc ctgttaatac ggtgataaca
    69301 acaatgaaaa aattaaccat tggcttaatt ggtaatccaa attctggcaa gacaacctta
    69361 tttaaccagc tcactggcgc acgtcagcgt gtaggtaact gggctggcgt taccgtcgaa
    69421 cgtaaagaag ggcaattctc caccaccgat catcaggtca cgctggtgga cctgcccggc
    69481 acctattctc tgaccaccat ctcatcgcag acctcgctcg atgagcaaat cgcctgtcac
    69541 tacattttga gtggcgacgc cgacctgctg attaacgtgg tggatgcgtc taaccttgag
    69601 cgtaacctgt acctgacgct gcaactgctg gaactcggca ttccctgcat cgtggcgctg
    69661 aacatgctcg acattgccga gaagcaaaat attcgtattg aaattgatgc tctatcggca
    69721 cgtcttggct gcccggtgat cccgctggtt tcaacccgtg gtcgcggtat tgaagcgctc
    69781 aagctggcga ttgatcgcta taaagctaac gagaatgtgg aactggtgca ttacgcacag
    69841 ccgctactca acgaagcaga ttcactggca aaagtgatgc cttccgacat cccgctgaaa
    69901 caacgtcgct ggctgggcct gcaaatgctg gaaggcgata tctacagccg cgcctacgcc
    69961 ggtgaagcgt cgcagcatct ggatgccacc ctcgcccgtc tgcgtaatga aatggatgat
    70021 ccggcgctgc acattgccga tgcgcgttac cagtgcattg ctgccatctg tgatgtggta
    70081 agcaacaccc tgacggcaga acccagccgt ttcaccactg cggtagataa aatcgtgctc
    70141 aaccgtttcc tcggtctgcc gattttcctc tttgtgatgt acctgatgtt cctgctggct
    70201 atcaacatcg gcggggcgtt acagccgctg tttaacgtcg gctccgtggc gctatttgtg
    70261 catggtattc aatggattgg ctacacgctc cacttcccgg actggctgac tatcttcctc
    70321 gcccagggcc tgggtggcgg tattaacacc gtgctgccac tggtgccgca gatcggcatg
    70381 atgtacctgt tcctctcctt ccttgaggac tccggctata tggcgcgtgc agcgtttgtg
    70441 atggaccgcc tgatgcaggc gctgggcttg ccggggaaat cctttgtgcc gctgatcgtc
    70501 ggtttcggtt gtaacgtacc gtcggtaatg ggcgcacgta cgcttgatgc accgcgtgaa
    70561 cgtctgatga ccatcatgat ggcaccgttt atgtcctgcg gcgcgcgtct ggctatcttc
    70621 gcagtattcg cggctgcctt cttcgggcag aacggtgccc tggcggtttt ctcgctgtat
    70681 atgctcggta tcgtgatggc ggtgctgact ggcctgatgc tcaagtacac catcatgcgc
    70741 ggtgaagcga cgccgtttgt catggagctg ccggtctacc atgtaccaca cgttaaaagc
    70801 ctgattatcc agacctggca gcgtctgaag ggcttcgttc tgcgtgctgg taaagtgatt
    70861 atcatcgtca gcattttcct gagcgctttc aacagcttct cgttgagcgg gaaaatcgtc
    70921 gataacatca acgactcggc gctggcttcc gtcagccggg tgataacccc tgtcttcaag
    70981 ccgattggcg tacatgaaga taactggcag gcaacggttg gcctgtttac gggcgcaatg
    71041 gcaaaagaag tggtggtggg tacgctcaac accctctaca ccgcagaaaa tattcaggac
    71101 gaagagttca atccggcgga attcaacctc ggtgaagagc tgttcagtgc ggtagatgaa
    71161 acctggcaga gcctgaaaga caccttcagc cttagcgtac tgatgaaccc cattgaagct
    71221 agcaaaggca acggtgaaat gggtaccggg gcgatgggcg tgatggatca gaaattcggt
    71281 agcgcagctg ccgcatacag ctacctgatt ttcgtcctgc tgtatgtacc atgtatctcg
    71341 gtgatgggag ccatcgcccg tgaatcaagc cgtggctgga tgggcttctc catcctgtgg
    71401 gggctgaata tcgcttactc actggcaaca ttgttctatc aggtcgccag ctacagtcag
    71461 cacccaactt acagcctggt gtgcattctg gcggtaatcc tgtttaacat cgtagctatc
    71521 ggtctgctgc gccgcgcgcg tagccgggtg gatatcgaac tgctggcaac ccgcaagtcg
    71581 gtaagcagtt gctgcgcagc cagcaccacc ggtgattgcc attaatggct tcacttattc
    71641 aggtacgtga tttgctggcg ttacggggcc gtatggaagc gacccagata agccagacat
    71701 tgaacactcc gcagccaatg attaacgcca tgctgcaaca gctggaaagc atgggcaaag
    71761 ccgtgcggat tcaggaagaa cctgacggct gcctctctgg cagttgtaaa agctgcccgg
    71821 aaggcaaagc ctgtctgcgc gagtggtggg cgctgcgtta accatactcc atcgcctgtt
    71881 ttgaaaagcc cggtatgcgt ttgcatccgg gcttttttgc gtgcggcttt ccataaaaat
    71941 gcaattcttg cagcgcaacg taagttcctc tgaaagcgtc tcgcagggat gaaaactcgc
    72001 taatgcacag atgtggagtg gcgcgtagag tcgcggcatt caaacaacag gtgaaggaac
    72061 gccatgagta aaaagcagag ttccacccca catgatgcgc tgttcaaact ctttttacgc
    72121 caaccggaga cggcacgcga ttttcttgcg tttcatttac cggcacccat tcacgcgctc
    72181 tgtgatatga aaaccctcaa gctggagtcg aggagcttta ttgatgacga tctgcgtgaa
    72241 agctattccg atgtgctgtg gtcggtgaaa acggaacaag gaccaggata catctattgt
    72301 ctgattgaac atcaaagcac ctcaaacaaa ctgatcgcat ttcgcatgat gcgttacgct
    72361 attgccgcaa tgcaaaatca ccttgatgct ggatacaaaa cgttgccgat ggtggtgcca
    72421 ttgttgtttt accacggtat tgaaagcccc tatccctatt cgctgtgttg gctggattgt
    72481 ttcgccgatc ccaaactggc aaggcagctt tatgcctccg catttccgct gattgatgtc
    72541 accgtcatgc ctgatgatga aatcatgcag caccgacgta tggcgctgct ggagttaatt
    72601 caaaaacata ttcgtcaacg cgatctgatg gggctggtag agcaaatggc ctgcttatta
    72661 agtagtggat acgctaatga cagacaaatc aaagggctgt ttaattacat actgcaaact
    72721 ggcgacgctg tacgttttaa cgattttatc gacggcgttg ccgaacgttc accgaaacac
    72781 aaggagagtt taatgactat tgcggaaaga ttgcggcagg agggggaaca atccaaagcc
    72841 ctgcatatag ccaaaataat gcttgaatcc ggagtccctc ttgcagacat catgcgattt
    72901 accgggctgt cagaagaaga gttggctgcg gcgagtagac tggccccctg aatctccaga
    72961 caaccagtat cacttaa
//
LOCUS       DAEBXN010000003        70712 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.003, whole genome shotgun sequence.
ACCESSION   DAEBXN010000003 DAEBXN010000000
VERSION     DAEBXN010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 70712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70712
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.003"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..795
                     /locus_tag="KJL99_000172"
     CDS             <1..795
                     /locus_tag="KJL99_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBD4435059.1"
                     /translation="WLPRSLVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLD
                     DDNNHNSSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAF
                     ALIDANGASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQ
                     FVVPNRGAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILY
                     LSGISSKSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            806..1897
                     /locus_tag="KJL99_000173"
     CDS             806..1897
                     /locus_tag="KJL99_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBD4435060.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVN
                     MRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(1940..2800)
                     /gene="rsmI"
                     /locus_tag="KJL99_000174"
     CDS             complement(1940..2800)
                     /gene="rsmI"
                     /locus_tag="KJL99_000174"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HBD4435061.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRTLEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            2865..4901
                     /gene="lpoA"
                     /locus_tag="KJL99_000175"
     CDS             2865..4901
                     /gene="lpoA"
                     /locus_tag="KJL99_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HBD4435062.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADSVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSALGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            4859..5254
                     /locus_tag="KJL99_000176"
     CDS             4859..5254
                     /locus_tag="KJL99_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HBD4435063.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGLTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            5274..5864
                     /gene="diaA"
                     /locus_tag="KJL99_000177"
     CDS             5274..5864
                     /gene="diaA"
                     /locus_tag="KJL99_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HBD4435064.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            5874..6449
                     /gene="yraP"
                     /locus_tag="KJL99_000178"
     CDS             5874..6449
                     /gene="yraP"
                     /locus_tag="KJL99_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HBD4435065.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(6563..7603)
                     /locus_tag="KJL99_000179"
     CDS             complement(6563..7603)
                     /locus_tag="KJL99_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBD4435066.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(7676..8311)
                     /locus_tag="KJL99_000180"
     CDS             complement(7676..8311)
                     /locus_tag="KJL99_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HBD4435067.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            8439..8957
                     /gene="yhbO"
                     /locus_tag="KJL99_000181"
     CDS             8439..8957
                     /gene="yhbO"
                     /locus_tag="KJL99_000181"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HBD4435068.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGVEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(8937..9380)
                     /locus_tag="KJL99_000182"
     CDS             complement(8937..9380)
                     /locus_tag="KJL99_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HBD4435069.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            9431..9733
                     /gene="yhbQ"
                     /locus_tag="KJL99_000183"
     CDS             9431..9733
                     /gene="yhbQ"
                     /locus_tag="KJL99_000183"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HBD4435070.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(9720..10223)
                     /locus_tag="KJL99_000184"
     CDS             complement(9720..10223)
                     /locus_tag="KJL99_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBD4435071.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFLVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(10217..10741)
                     /locus_tag="KJL99_000185"
     CDS             complement(10217..10741)
                     /locus_tag="KJL99_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HBD4435072.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNTQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGVYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            10950..11945
                     /locus_tag="KJL99_000186"
     CDS             10950..11945
                     /locus_tag="KJL99_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBD4435073.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDCERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQAAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            11954..12832
                     /locus_tag="KJL99_000187"
     CDS             11954..12832
                     /locus_tag="KJL99_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBD4435074.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDCLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAVPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12913..13920
                     /locus_tag="KJL99_000188"
     CDS             12913..13920
                     /locus_tag="KJL99_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HBD4435075.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHLPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYDV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(14038..15282)
                     /gene="mtr"
                     /locus_tag="KJL99_000189"
     CDS             complement(14038..15282)
                     /gene="mtr"
                     /locus_tag="KJL99_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HBD4435076.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(15436..17325)
                     /gene="deaD"
                     /locus_tag="KJL99_000190"
     CDS             complement(15436..17325)
                     /gene="deaD"
                     /locus_tag="KJL99_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HBD4435077.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(17318..17398)
                     /gene="yrbN"
                     /locus_tag="KJL99_000191"
     CDS             complement(17318..17398)
                     /gene="yrbN"
                     /locus_tag="KJL99_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HBD4435078.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(17505..18389)
                     /gene="nlpI"
                     /locus_tag="KJL99_000192"
     CDS             complement(17505..18389)
                     /gene="nlpI"
                     /locus_tag="KJL99_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HBD4435079.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(18498..20633)
                     /gene="pnp"
                     /locus_tag="KJL99_000193"
     CDS             complement(18498..20633)
                     /gene="pnp"
                     /locus_tag="KJL99_000193"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HBD4435080.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20880..21149)
                     /gene="rpsO"
                     /locus_tag="KJL99_000194"
     CDS             complement(20880..21149)
                     /gene="rpsO"
                     /locus_tag="KJL99_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HBD4435081.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(21298..22242)
                     /gene="truB"
                     /locus_tag="KJL99_000195"
     CDS             complement(21298..22242)
                     /gene="truB"
                     /locus_tag="KJL99_000195"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HBD4435082.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERIVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(22242..22643)
                     /gene="rbfA"
                     /locus_tag="KJL99_000196"
     CDS             complement(22242..22643)
                     /gene="rbfA"
                     /locus_tag="KJL99_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HBD4435083.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(22807..25455)
                     /gene="infB"
                     /locus_tag="KJL99_000197"
     CDS             complement(22807..25455)
                     /gene="infB"
                     /locus_tag="KJL99_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HBD4435084.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSNQQDDMTKN
                     AQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDNAEPTEDSS
                     DYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADREEARAAVRG
                     GKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIKAMMKLGAM
                     ATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAPVVTIMGHV
                     DHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAAFTSMRARG
                     AQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPDRVKNELSQ
                     YGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMASGAVIESFL
                     DKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSIPVEILGLS
                     GVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMTEGEVHEVN
                     IVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASNAILVGFNV
                     RADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLAEVRDVFKS
                     PKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVRNGMECGIG
                     VKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(25480..26967)
                     /gene="nusA"
                     /locus_tag="KJL99_000198"
     CDS             complement(25480..26967)
                     /gene="nusA"
                     /locus_tag="KJL99_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HBD4435085.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26995..27447)
                     /gene="rimP"
                     /locus_tag="KJL99_000199"
     CDS             complement(26995..27447)
                     /gene="rimP"
                     /locus_tag="KJL99_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HBD4435086.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(27654..27730)
                     /locus_tag="KJL99_000200"
     tRNA            complement(27654..27730)
                     /locus_tag="KJL99_000200"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27694..27696),aa:Met,seq:cat)
     gene            28078..29421
                     /gene="argG"
                     /locus_tag="KJL99_000201"
     CDS             28078..29421
                     /gene="argG"
                     /locus_tag="KJL99_000201"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HBD4435087.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGLPQVENMENKGQ"
     gene            complement(29429..31036)
                     /locus_tag="KJL99_000202"
                     /pseudo
     CDS             complement(29429..31036)
                     /locus_tag="KJL99_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011378876.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            complement(31514..31600)
                     /locus_tag="KJL99_000203"
     tRNA            complement(31514..31600)
                     /locus_tag="KJL99_000203"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31564..31566),aa:Leu,seq:gag)
     gene            complement(31615..31947)
                     /gene="secG"
                     /locus_tag="KJL99_000204"
     CDS             complement(31615..31947)
                     /gene="secG"
                     /locus_tag="KJL99_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HBD4435088.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNRGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(32175..33512)
                     /gene="glmM"
                     /locus_tag="KJL99_000205"
     CDS             complement(32175..33512)
                     /gene="glmM"
                     /locus_tag="KJL99_000205"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HBD4435089.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(33505..34353)
                     /gene="folP"
                     /locus_tag="KJL99_000206"
     CDS             complement(33505..34353)
                     /gene="folP"
                     /locus_tag="KJL99_000206"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HBD4435090.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(34443..36377)
                     /gene="ftsH"
                     /locus_tag="KJL99_000207"
     CDS             complement(34443..36377)
                     /gene="ftsH"
                     /locus_tag="KJL99_000207"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBD4435091.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(36477..37106)
                     /gene="rlmE"
                     /locus_tag="KJL99_000208"
     CDS             complement(36477..37106)
                     /gene="rlmE"
                     /locus_tag="KJL99_000208"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HBD4435092.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            37232..37525
                     /gene="yhbY"
                     /locus_tag="KJL99_000209"
     CDS             37232..37525
                     /gene="yhbY"
                     /locus_tag="KJL99_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HBD4435093.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(37681..38157)
                     /gene="greA"
                     /locus_tag="KJL99_000210"
     CDS             complement(37681..38157)
                     /gene="greA"
                     /locus_tag="KJL99_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HBD4435094.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            38405..39838
                     /gene="dacB"
                     /locus_tag="KJL99_000211"
     CDS             38405..39838
                     /gene="dacB"
                     /locus_tag="KJL99_000211"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HBD4435095.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPSDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     KPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(40024..41196)
                     /gene="cgtA"
                     /locus_tag="KJL99_000212"
     CDS             complement(40024..41196)
                     /gene="cgtA"
                     /locus_tag="KJL99_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HBD4435096.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(41212..42177)
                     /locus_tag="KJL99_000213"
     CDS             complement(41212..42177)
                     /locus_tag="KJL99_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBD4435097.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(42304..42561)
                     /gene="rpmA"
                     /locus_tag="KJL99_000214"
     CDS             complement(42304..42561)
                     /gene="rpmA"
                     /locus_tag="KJL99_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HBD4435098.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(42582..42893)
                     /gene="rplU"
                     /locus_tag="KJL99_000215"
     CDS             complement(42582..42893)
                     /gene="rplU"
                     /locus_tag="KJL99_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HBD4435099.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            43151..44122
                     /gene="ispB"
                     /locus_tag="KJL99_000216"
     CDS             43151..44122
                     /gene="ispB"
                     /locus_tag="KJL99_000216"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HBD4435100.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            44349..44627
                     /gene="sfsB"
                     /locus_tag="KJL99_000217"
     CDS             44349..44627
                     /gene="sfsB"
                     /locus_tag="KJL99_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HBD4435101.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(44675..45934)
                     /gene="murA"
                     /locus_tag="KJL99_000218"
     CDS             complement(44675..45934)
                     /gene="murA"
                     /locus_tag="KJL99_000218"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBD4435102.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(45989..46243)
                     /gene="ibaG"
                     /locus_tag="KJL99_000219"
     CDS             complement(45989..46243)
                     /gene="ibaG"
                     /locus_tag="KJL99_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HBD4435103.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(46403..46696)
                     /gene="mlaB"
                     /locus_tag="KJL99_000220"
     CDS             complement(46403..46696)
                     /gene="mlaB"
                     /locus_tag="KJL99_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HBD4435104.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(46696..47331)
                     /gene="mlaC"
                     /locus_tag="KJL99_000221"
     CDS             complement(46696..47331)
                     /gene="mlaC"
                     /locus_tag="KJL99_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HBD4435105.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(47350..47901)
                     /gene="mlaD"
                     /locus_tag="KJL99_000222"
     CDS             complement(47350..47901)
                     /gene="mlaD"
                     /locus_tag="KJL99_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HBD4435106.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTESTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(47906..48688)
                     /gene="mlaE"
                     /locus_tag="KJL99_000223"
     CDS             complement(47906..48688)
                     /gene="mlaE"
                     /locus_tag="KJL99_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HBD4435107.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(48696..49505)
                     /gene="mlaF"
                     /locus_tag="KJL99_000224"
     CDS             complement(48696..49505)
                     /gene="mlaF"
                     /locus_tag="KJL99_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HBD4435108.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQTNPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            49715..50680
                     /locus_tag="KJL99_000225"
     CDS             49715..50680
                     /locus_tag="KJL99_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HBD4435109.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQGTDSLTREQLA
                     ELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMALTAIAIGTSL
                     PELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDYSVML
                     LVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            50694..51680
                     /gene="kdsD"
                     /locus_tag="KJL99_000226"
     CDS             50694..51680
                     /gene="kdsD"
                     /locus_tag="KJL99_000226"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HBD4435110.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPSEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            51701..52267
                     /gene="kdsC"
                     /locus_tag="KJL99_000227"
     CDS             51701..52267
                     /gene="kdsC"
                     /locus_tag="KJL99_000227"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HBD4435111.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            52264..52839
                     /gene="lptC"
                     /locus_tag="KJL99_000228"
     CDS             52264..52839
                     /gene="lptC"
                     /locus_tag="KJL99_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HBD4435112.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            52808..53365
                     /gene="lptA"
                     /locus_tag="KJL99_000229"
     CDS             52808..53365
                     /gene="lptA"
                     /locus_tag="KJL99_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HBD4435113.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            53372..54097
                     /gene="lptB"
                     /locus_tag="KJL99_000230"
     CDS             53372..54097
                     /gene="lptB"
                     /locus_tag="KJL99_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HBD4435114.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            54145..55578
                     /gene="rpoN"
                     /locus_tag="KJL99_000231"
     CDS             54145..55578
                     /gene="rpoN"
                     /locus_tag="KJL99_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HBD4435115.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            55601..55888
                     /gene="hpf"
                     /locus_tag="KJL99_000232"
     CDS             55601..55888
                     /gene="hpf"
                     /locus_tag="KJL99_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HBD4435116.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            56006..56497
                     /gene="ptsN"
                     /locus_tag="KJL99_000233"
     CDS             56006..56497
                     /gene="ptsN"
                     /locus_tag="KJL99_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HBD4435117.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            56543..57397
                     /gene="rapZ"
                     /locus_tag="KJL99_000234"
     CDS             56543..57397
                     /gene="rapZ"
                     /locus_tag="KJL99_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HBD4435118.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            57394..57666
                     /gene="npr"
                     /locus_tag="KJL99_000235"
     CDS             57394..57666
                     /gene="npr"
                     /locus_tag="KJL99_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HBD4435119.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            57879..58511
                     /gene="yrbL"
                     /locus_tag="KJL99_000236"
     CDS             57879..58511
                     /gene="yrbL"
                     /locus_tag="KJL99_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HBD4435120.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(58508..59235)
                     /gene="mtgA"
                     /locus_tag="KJL99_000237"
                     /pseudo
     CDS             complement(58508..59235)
                     /gene="mtgA"
                     /locus_tag="KJL99_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
     gene            complement(59232..59885)
                     /gene="elbB"
                     /locus_tag="KJL99_000238"
     CDS             complement(59232..59885)
                     /gene="elbB"
                     /locus_tag="KJL99_000238"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HBD4435121.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(60115..62451)
                     /gene="arcB"
                     /locus_tag="KJL99_000239"
     CDS             complement(60115..62451)
                     /gene="arcB"
                     /locus_tag="KJL99_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HBD4435122.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(62547..63476)
                     /locus_tag="KJL99_000240"
     CDS             complement(62547..63476)
                     /locus_tag="KJL99_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709009.2"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HBD4435123.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETSVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            64151..68611
                     /gene="gltB"
                     /locus_tag="KJL99_000241"
     CDS             64151..68611
                     /gene="gltB"
                     /locus_tag="KJL99_000241"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HBD4435124.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKSLYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            68624..70042
                     /gene="gltD"
                     /locus_tag="KJL99_000242"
     CDS             68624..70042
                     /gene="gltD"
                     /locus_tag="KJL99_000242"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HBD4435125.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            70226..70705
                     /locus_tag="KJL99_000243"
     CDS             70226..70705
                     /locus_tag="KJL99_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HBD4435126.1"
                     /translation="MESLSERTSTGYQQIHNGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIDRLSKDLSRRYKRGVSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKLRGCLIFPQLLYCISYFRFVYSCK"
BASE COUNT        17314 a        19175 c        17559 g        16664 t
ORIGIN      
        1 tggttaccgc gtagcctggt ttcctatcag atgaccagcc agaaagatcg cccaacccaa
       61 catgaaatgc gtcttgatgg ctcactgctg gatgatggtc gcctgagcta tagcctggaa
      121 caaagtctgg atgacgataa caaccataac agtagcctga acgccagtta ccgttcacct
      181 tatggaacct tcagtgccgg atacagttac ggtaatgaca gcagccaata caattacggc
      241 gttaccggcg gcgtggttat ccatcctcat ggcgtgacgc tctcgcaata tctgggcaac
      301 gcttttgcgc ttatcgatgc taacggggct tctggcgtga ggatacaaaa ctatccgggg
      361 attgctactg atccctttgg ctatgcagtg gttccttatc tcacgactta tcaggaaaac
      421 cgtctctcgg tagatactac gcagctgccc gataacgtcg atcttgaaca aacaacacag
      481 tttgtggtgc ccaacagagg tgcaatggta gcggcgcgtt tcaacgccaa tatcggttat
      541 cgcgtacttg ttacagtcag cgatcgcaac ggtaaaccgt tgccctttgg cgctcttgcc
      601 agcaacgatg atacggggca acaaagtatc gtcgatgagg gcggcatact atatctctct
      661 gggatatcga gtaaatcaca aagctggact gtacgctggg gaaatcaggc agatcaacaa
      721 tgtcagtttg cttttagtac accggattca gaaccaacaa cctctgtatt acaaggcaca
      781 gcgcagtgcc attaaggata aaaaaatgaa aagagcgcct cttataacag gacttttgtt
      841 gatatccaca tcctgcgctt atgcctcctc aggagggtgt ggagccgaca gcactagcgg
      901 tgcgacaaat tacagcagtg tggttgatga tgttacggtg aaccagacag ataacgtgac
      961 aggacgggag tttacctctg caacgctaag tagcactaac tggcaatacg cctgttcctg
     1021 ctctgcgggt aaggcagtta aacttgtcta tatggtcagc cccgtactta ccaccactgg
     1081 acatcagaca ggatattaca aactcaatga cagcctggat attaaaacca cattacaggc
     1141 aaacgacatt ccaggactca caaccgacca ggttgtctct gttaacaccc gattcacaca
     1201 gataaaaagc agcaccgtat attctgctgc aacccaaacg ggtgtttgcc agggtgacac
     1261 gtctcgttat ggacccgtta atattggtgc aaataccacc tttaccctgt atgtcaccaa
     1321 gccatttctc ggctcgatga ccattccgaa aacggatatt gccgtcatta aaggcgcgtg
     1381 ggtcgatgga atgggaagcc cgtctacagg tgacttccat gatttagtca agttatcgat
     1441 tcagggaaat ctcaccgccc cacagtcgtg caaaattaat cagggcgatg ttattaaagt
     1501 taattttgga ttcatcaatg gtcagaagtt taccacccgc aatgccatgc cagacggttt
     1561 tactccagta gactttgata tcacttatga ctgtggtgat acttcaaaga ttaaaaactc
     1621 gttgcaaatg cgcatcgacg gtacaactgg ggtagtagac cagtacaacc tggtcgccag
     1681 gcgaagaagt tcagacaatg tgcccgatgt cggtattcgt attgaaaatc tcggcggcgg
     1741 agttgcaaat attccttttc agaacggtat ccttcccgtt gatccttccg ggcatggcac
     1801 cgtcaatatg cgcgcctggc cagttaatct ggtcggtggt gagctggaaa caggaaaatt
     1861 tcagggcaca gccaccatta ccgtcatggt gcggtaaaag aagacgttgc gttttgatta
     1921 acgcaacgct tcggcactgt tacccctgct gctccagcgc atacttatac agcgcatttt
     1981 tcttcacgcc atgaatttct gcggccagcg ccgccgcttt tttcagcggc agttctgcct
     2041 gtagcagcgc cagcgtgcgc agggcatcgg cgggtaagtc ttcttcctgt gctttatgac
     2101 cttcgacaat cagcaccatt tcgcctttgc gacggttttc atcttccttt acccacgcca
     2161 gcagctcgcc aacgggcgcg ccgtgaatgg tttcccaggt tttggtcagc tcacgcgcca
     2221 gaaccacgta gcgggattcg cctaataccg caacgatatc ttccaggcta tctaacagac
     2281 ggtgggtaga ttcataaaaa atcagcgtgc gcggctccgc ttcaatggct tttagcgcat
     2341 cacggcggcc ttttgatttg gcaggtaaaa agccttcgta acagaaacgg tcagatggta
     2401 aacccgcggc gcttaacgca gtgatagcag cacacggtcc gggtagcggc accacacgaa
     2461 tccccgcttc acggcaggta cgcaccagat ggtagccagg atcgttaatt agcggcgttc
     2521 cggcatcgga aaccagcgca atgttttgcc cctcttgcag ctttgccagc agcgtttcgg
     2581 ctttttgttg ttcgttatgg tcgtgcagcg caaacaaccg ggcattaatc ccaaaatgtt
     2641 gcagcaataa accggtgtga cgagtatcct cggcggcaat cagatcaacg gcctgtaata
     2701 cctctaacgt acgctgggtg atatccgcca gattgccgat tggcgtcggt acaatgtaaa
     2761 gctggccctg agaattatcc gccgattggt gttgtttcat tgtgtcgtcc gtattgccga
     2821 tttaatattg agcattgcgt aaaaaaaata tcactggata cattatggta ccctcaacat
     2881 tttctcgttt gaaagccgcg cgttgtctgc ctgttgttct ggcagccctg attttcgccg
     2941 gttgtggcac ccatactccc gatcagtcca ctgcttatat gcagggcacg gcgcaggctg
     3001 attctgcctt ttatcttcag cagatgcagc aaagctctga tgataccagg atcaactggc
     3061 aattactcgc cattcgtgca ctggtgaaag aaggtaaaac cgggcaggcg gttgagttgt
     3121 ttaaccaact accgcaagaa ctgaacgatt ctcagcgtcg cgagaaaaca ctgctggcgg
     3181 cagagattaa actggcgcag aaagattttg ctggcgcgca aaacttgctg gcgaaaatca
     3241 cacctgccga tttagaacaa aaccagcaag cgcgttactg gcaggcaaaa atcgatgcca
     3301 gccaggggcg tccttccatt gatttactgc gcgcgttaat tgctcaggaa ccgctgcttg
     3361 gcgcgaaaga aaaacagcag aatattgatg ccacctggca ggcgctctcc tccatgactc
     3421 aggaacaggc gaatacgctg gtgatcaacg ccgacgaaaa tattctgcaa ggctggctgg
     3481 atctgcagcg cgtctggttt gataaccgta acgatcccga catgatgaaa gccgggatcg
     3541 ccgactggca gaaacgttat ccgaacaatc cgggcgcgaa aatgctgcca acgcagttgg
     3601 ttaacgtaaa agcgtttaaa ccagcctcga ccaacaaaat cgccctgctg ttgccgctga
     3661 atggccaggc agcggtattt ggtcgcacta ttcagcaagg ctttgaagcc gcgaaaaata
     3721 tcggcactca gccagtggcg gctcaggtag ctgccgcacc tgccgcagac gtagcagaac
     3781 aacctcagcc gcaaactgcg gatagcgttg ccagcccggc acaagcctcg gttagcgatc
     3841 tgaccggtga tcagcctgcg gcccagccgg tgcctgtaag cgccccggcg acaagcaccg
     3901 cagcggtaag cgcacccgca aatccatccg cagagctgaa aatctacgat acctcatcac
     3961 aaccacttag ccagatctta agccaggttc agcaggatgg cgcgagtatt gtggttggtc
     4021 cgttgctgaa aaataacgtt gaagagttgc tgaagagcaa caccccgctg aacgtactgg
     4081 cactgaacca gccggagaat atcgaaaacc gcgtcaatat ttgttacttc gcgctttcac
     4141 cggaagacga agcgcgcgat gcagcgcgtc atattcgtga ccagggtaaa caagcgccgc
     4201 tggtgctgat cccacgcagt gcattgggcg atcgcgtagc caatgcgttt gcgcaagagt
     4261 ggcagaaact gggcggcggc accgttctgc aacaaaaatt tggttccacc agcgaattac
     4321 gcgcgggtgt taacggcggt tctggtattg ctttaacggg tagcccgatt actcccagag
     4381 cgacaaccga ctccggcatg acgaccaaca atccaacgct gcaaaccacg ccaaccgatg
     4441 accagttcac caataatggc ggtcgtgtcg atgcggtcta cattgtggca acgccgggtg
     4501 aaatcgcttt tatcaaaccg atgatcgcca tgcgtaacgg tagccagagc ggtgcaacgc
     4561 tgtacgccag ttcccgcagc gcacaaggca ccgcaggccc ggatttccga ctggagatgg
     4621 aaggcttgca gtacagcgaa atcccgatgc tggcaggcgg taatctgccg ttaatgcagc
     4681 aggcgctcag cgcggtgaac aacgattatt cgctggctcg catgtatgcg atgggcgttg
     4741 atgcctggtc gttggcaaat catttctcac aaatgcgcca ggttcagggt tttgaaatca
     4801 acggtaatac cggaagtctg acggctaacc cggattgcgt gattaacagg aagttatcat
     4861 ggctacagta ccaacaaggt caggtagtcc ccgccagtta accaccaaac agaccggcga
     4921 tgcgtgggaa gcacaagcgc gtcgctggct ggaaggcaaa ggactgcggt ttatcgccgc
     4981 taacgtgaac gagcgtggcg gcgagatcga tctgataatg cgtgaaggcc tgaccaccgt
     5041 ttttgtcgag gtacgctatc gccgctctgc gctttatggc ggcgcggcag ccagtgtgac
     5101 ccgcagcaaa caacacaaat tattacagac tgcccgcttg tggctcgcgc gtcataatgg
     5161 gagttttgat actgtggatt gccggttcga tgtggtagcc ttcaccggga atgaggttga
     5221 gtggattaag gatgccttta atgaccactc ataattaagg tttaaggatt agcgtgcaag
     5281 aaagaattaa agcttgcttc actgaaagca ttcaaactca aattgcggcg gcagaggcgc
     5341 ttccggatgc catctcccgt gcagccatga cgctggttca gtctctgctc aatggcaaca
     5401 aaatcctctg ttgtggtaat ggaacttccg ctgccaatgc acagcatttt gctgccagca
     5461 tgatcaaccg tttcgaaacg gagcggccca gcttacctgc cattgcacta aatactgata
     5521 atgttgtctt aacggcgatt gccaacgatc gcttacatga tgaagtgtat gcaaaacagg
     5581 tgcgggcgct gggtcatgcg ggagatgtat tgttagccat ttccacccgt ggcaacagcc
     5641 gcgatattgt taaagcagtt gaagccgccg ttacgcgtga tatgaccatt gtggcattga
     5701 ccggctatga cggcggcgaa cttgcaggtt tgttagggcc acaggatgtg gagatccgca
     5761 ttccttcgca tcgtagtgct cgcattcagg aaatgcatat gctgacggta aattgcctgt
     5821 gcgatctgat cgataacacg cttttccctc accaggatga ttaaggagaa tacatgaagg
     5881 cattatcgcc aatcgcagtc cttatttccg cgctgctgtt gcaaggttgt gttgccgctg
     5941 ccgtagtggg taccgctgct gtgggtacca aagccgcaac tgacccacgc agtgtcggca
     6001 cccaggtgga cgatggtacc ctggaagtgc gcgtgaacag cgcattgtcg aaagacgaac
     6061 agattaagaa agaaacccgt attaatgtca cggcctatca gggcaaagtg ctgctggttg
     6121 ggcagtcacc aaatgctgaa ctttcggctc gcgccaaaca gattgctatg ggcgtagacg
     6181 gtgccaacga agtgtataac gagattcgtc agggccagcc gattggtctg ggcgaagcat
     6241 ctaacgatac gtggatcacc accaaagtgc gttcgcagct cttaaccagc gacctggtga
     6301 aatcgtccaa cgtgaaagtg accaccgaaa acggtgaagt gttcctgatg gggctggtga
     6361 ctgaacgtga agcgaaagcg gcggcagata ttgccagccg ggtgagcggc gtaaagcggg
     6421 ttaccacagc gtttacgttt attaaatagc agccccttgt aatgcctgat gcgacgcttg
     6481 ccgcgtctta tcaggcctac aacgatgcaa accgtaggtc ggataaggcg tttacgccgc
     6541 atccgacatg ttttccctca aatcagaaca acagcgccag cccgccgaca atcacaccag
     6601 acactgccac catcgcccct gtcagccata aggctttcgc cgggaacgct ttgcgcagca
     6661 taatcagtga cggcaaactc actgccggga gcgtcatcaa caatgccagc gccggagcgg
     6721 ttcccatacc tgccagcatc atcgtttgta caatcggaat ttctgctgcc gtgggaatga
     6781 caaacaagca tcctgctacc gccatcgcca ccacccacat caggctgtta tcgacagcac
     6841 catcggcatg ggggaataac cagacgcgtg cggcacccaa caccagtact gcaaggatgt
     6901 aaaccgggat cgtactccag aaaagtgtcc atagcgccct gccccagcgg ctaaaaaaac
     6961 cgctctgtgc ttccggtatg tcaatttcga ccggtgcctg cgtttgcggt gtttcacgca
     7021 cccatttttg caccagcgtc gcaatcagca acaccatcac cagcccggcc accagacgaa
     7081 tcgccgcaaa accccagccg aggacaaacc ccataaacac cagcgtcgcc gggtttaaca
     7141 ccggattgcc catccagaat gccagcgcac cgcccatcga tacctgttgg cgacgcattc
     7201 ccgccgcgac cggagccgca cagcaggtac acatcatgcc cggcagcgaa aacagcgttc
     7261 ccagcagcgt gccgcgaaag cgcgattgcc caagcgtacg caacaaccag tcacgcggga
     7321 tcagcacctg aatcaacgaa ccgaggatca cccccagcac cgccgctttc catactgcga
     7381 ggaaatagat catcgcgtaa tccaacgccg cttgccacgg attagcatcc gcctgcgcaa
     7441 ggatagattt accgatactg tgggtttcgg ctgcagtaaa cgctttgccg tagtaaggtt
     7501 gccatttcac ataccagaga ccggcaatga caacgagaaa gaaaagcgcg ggtttccacc
     7561 actgaatggg cgttgccgcc tgagatgaag actgaccagt catagcattc cccaggaata
     7621 agttatgata attgagcgcg tgaatattac gctcactatc aattcttggg aataattatt
     7681 cagctctttt gcgtaattct gaagagctta aaatcgtcac gccttcatgc tcaggccgca
     7741 tcgcctctgc cagcatgacc cgcgccacgt ctctcgcatc aatggatttc cagttacctg
     7801 gtaacaaacg gaacagcggc gcaaaaagcg tttcgttcat ccgctgtttg ctacgatcgc
     7861 ccagtaacat cgacgggcga gcaatggtca atttcggcca gttctgggca attaatgctt
     7921 cttccatctc ccctttgacg cggttataga aaaacggcga gtgggcattg gcacccatag
     7981 cactgaccac cagcatatgc tgcgcgccca gtcgccgccc ggttaatgcg gtatccacta
     8041 ccagcgtgta atcggcatga ataaacgcct ctttgctccc cgcttctcgc cgcgtggtgc
     8101 cgagacaaca aaacacaatg tcgatgggtt cggtgacctg cgccaacgcg tcactcagtt
     8161 gcggatcatg gggattaaac accccgggca tatcgcccaa cggacgtcgc gtcggcgcag
     8221 caatggcgtt aactttcggt tcattaatca acatccgcag caggtgaccg cccaccagcc
     8281 ccgttgcgcc tgtaatcagt acctgactca tcttcgttcc tttacagaat tgtctgcctt
     8341 gcgctccccg gctcatgcac cagtcttaat agaccatgag gtaattatcc ccgattgtgg
     8401 aaaattcgcc tcatccaatg caacaacgcg gaggaagcat gagtaagaaa attgccgttt
     8461 taatcactga tgaatttgaa gattcagaat ttacttcacc cgcagacgag ttccgtaaag
     8521 ccggacacga agtgattact attgaaaaac aagcgggtaa aacggtgaaa ggcaaaaaag
     8581 gagaagccag cgtgaccatc gataaatcca tcgatgaagt gacgccagcg gagtttgatg
     8641 ccctgttgct accgggtggt cattcaccgg attatctgcg tggcgacaac cgttttgtca
     8701 cctttacccg tgattttgtg aatagcggca aacctgtttt cgccatctgt cacggtccgc
     8761 agctgctgat cagcgccgat gtgattcggg ggcgcaaact gaccgcagtt aaaccgatca
     8821 ttattgatgt taaaaatgcg ggcgtggaat tttacgatca ggaagtcgtg gttgataaag
     8881 atcagctggt taccagccgg acaccggacg atctgccagc gtttaaccgc gaggcgttac
     8941 gcctgctcgg tgcctgagtc gcgtagccaa atcatttttt taccaaagcc cagcgtgttg
     9001 tcggtgaatt tgatttcatc gagccggatt tcccacaccg gggccgacag cattctggca
     9061 accggaaagc gacgattgta cgccttgcgc gcgaggtcgc tttcctcacc ttccagcctg
     9121 cggatctcac ctttaaactg cacaccgcga attaacgcta ccgttttcgg ctgaccgttt
     9181 accgttccgg caaccgcagc ctgcggccca ctcatctgcg cgtggcgcgt tttttcttcc
     9241 gtcaaaatgt agaaggcgac tttctgcgca tcaaaaagat aaaaggcatt agcgcaccag
     9301 agttcccctt cctgctgcac acaccaggtg acaacatgtt gttttgccag ccaacggctg
     9361 atggcgatga gtgtttccat tgctgttctc ttttatactg tgggcctgac cttaacatgc
     9421 gatgcagacg atgacaccct ggtttcttta cttgatccgt accgccgaca ataagcttta
     9481 taccgggatc accacggatg tcgaacgccg ctatcagcag caccaaagcg gcaaaggggc
     9541 gaaagcactg cgcgggaaag gagaactgac gctggcgttt tctgcgccag tgggcgaccg
     9601 ttcgctggca ctacgggcgg aatatcgcgt taagcaactg accaaacggc aaaaagagcg
     9661 tctggtagcg gaaggcgcag ggtttgcaga actgttgagc agcctgcaaa ccccggagat
     9721 taaaagcgat tgaaatgctc gtgatactca accagaccgg ttacgccatt cagcgcgtca
     9781 tccgctaaac gatgtaccag gaaggcgctt tcagtgcccg gccagcggca acgcagatcg
     9841 tgatgcgccg ccagttcaaa gccgaaacga ctgtacagcg ccggatcgcc cagcgtcacc
     9901 actgcggcat agccgaactc attaagcgaa tcgagtcctt catagaccag ttggcgtgcc
     9961 agcccctgtc cacggtattt ttcatcaacc gccaacggtg ccatgccgac ccattgcagg
    10021 tcttcgccct gcacatcaac cggactaaat gccacatagc caatgacctg accttcgtca
    10081 tctgtcgcca ccagccccag cgtcagaaag ccatcttcac gcagatcgtg aaccagcttc
    10141 gcttccgcat cactttcgaa tgagcgacgc agcagggcat caatacccgg cgcatcaatg
    10201 ggaatttcta ctcgaattag catggttcac ctaccgatgt ctgtttggtt tcaggcgcgg
    10261 ttttcattcc cgcctcaaca aaatccgcca gttgcagcag catcatgcgt aaggctttcg
    10321 gcatttgctc cagttcaatg gcgtccatca ggtttttcac atacaccccc agctccgtat
    10381 cgccttcaat caccagccga cgctggaaga agagcgtatc cggatcttgt ttacgcgccg
    10441 caatcatcag cagatcgctg gcgtcggcac taaaactcac atcagcttgc gtgttctggc
    10501 tgaccaccag tttgccattc accaccgagg taaaccattg caggtcaata tcacgcacat
    10561 gaatacttaa ccagcggcct tcaagaaact ccagctcgcc atcatccagc gcctgacgga
    10621 attgccagct taagacctgc tcaagaacct ggcgttttag cgcaaatggc gtcagtttta
    10681 ccggtacact caacagagat ggccccaaat gcacaatacg ggaacgcagt ttatccaaca
    10741 cgagttttac tccctgtttc aacaatcatc ctattttgcc atatcagaaa aataacatag
    10801 cggtataaat caacaattcc atatgaaatt gctgctacca ccaatacaac tttaactgcc
    10861 ttaaatcaaa aattgtcgca gcaaggttaa ctaaaatccc agttcgttaa catttttgcg
    10921 ttttgatagc gcaaccttca ggaaaaatta tggagctgct ctgccctgcc ggaaatctcc
    10981 cggcgcttaa ggcggccatc gaaaacggcg cagatgctgt ttatatcggg ctaaaagatg
    11041 ataccaatgc ccgtcacttc gccggcctta actttaccga gaaaaaattg caggaagcgg
    11101 tgagttttgt ccatcaacat cgccgcaaac ttcacatcgc gattaacact tttgcgcatc
    11161 cggacggtta cgcccgttgg cagcgtgccg tggatatggc ggcgcagctg ggtgccgacg
    11221 cgctgatcct cgccgacctc gccatgctgg agtacgccgc cgagcgttac ccgcatattg
    11281 aacgtcatgt gtcagtgcag gcttcggcga ccaatgaaga ggcgattaac ttttatcatc
    11341 gccattttga cgttgcccgc gtggtgctgc cgcgcgtgtt gtcgattcat caggtgaaac
    11401 agctggcacg ggttactcct gtcccgctgg aagtgtttgc tttcggcagc ctgtgtatta
    11461 tgtcggaagg ccgttgctat ctgtcgtcgt atctgacggg tgagtcgccc aacactgtgg
    11521 gtgcgtgttc tccggcccgt ttcgtgcgct ggcaacaaac gccgcagggg ctggaatccc
    11581 gcctgaacga agtgctgatc gaccgttatc aggacggcga aaacgcaggt tatccgacgc
    11641 tgtgtaaagg gcgttatctg gtggactgcg agcgctatca cgcgctggaa gaaccaacca
    11701 gtctcaatac cctggaactg ctgccggagt taatggctgc gaatattgct tcggtgaaaa
    11761 ttgaaggccg ccagcgtagc ccggcgtatg tcagccaggc ggcgaaagtc tggcgtcagg
    11821 ctatcgaccg ttgtaaggcc gatccgcaaa acttcgtacc gcaaagcgcg tggatggaga
    11881 cgctcgggtc gatgtccgaa ggcacgcaaa ccacccttgg cgcgtatcac cgtaaatggc
    11941 agtgagaaga gcaatgaaat attccttagg gccagtgctg tggtactggc caaaagagac
    12001 gctggaagat ttttatcagc aagccgccgc cagcagcgcc gacgtgattt atcttggtga
    12061 agcggtatgc agcaagcgcc gggcaaccaa agttggcgac tgtctggaga tggcaaaatc
    12121 gctcgccgga agtggcaagc agattgtgct ctccacgctg gcgctggtgc aggcatcctc
    12181 tgaactgggc gaactgaaac gctatgttga gaacggtgaa tttctgattg aagccagcga
    12241 tctcggcgtg gtgaatatgt gcgctgaacg caaactgccg ttcgtcgccg ggcacgcgct
    12301 gaactgctac aacgcggtga cgctgaaaat attgctcaaa cagggcatga tgcgctggtg
    12361 tatgccggtg gagctttccc gcgactggct ggtgaatctg cttaatcagt gcgatgagct
    12421 gggcattcgc aaccagtttg aagtggaagt cctgagctac ggtcatctac cgctggccta
    12481 ctccgcccgc tgctttaccg cgcgttcgga agaccgcccg aaagatgagt gtgaaacctg
    12541 ctgcattaag tatccgaacg ggcgcaacgt gctgtcgcag gaaaaccaac aagtgtttgt
    12601 actcaatggc attcagacca tgagcggcta cgtttacaac ctcggtaacg agctggcatc
    12661 catgcaggga ttggttgatg tagtccgcct gtcaccgcag ggcactgaca ctttcgcgat
    12721 gctcgacgcc ttccgcgcta atgaaaatgg cgctgtgcca ctgccgttga cggcaaacag
    12781 cgactgtaac ggctactggc ggcggctggc aggactggag ctgcaagcct aagtaaatag
    12841 ctcactttgt taacaacttt aactattctt taatgcagta ttaaagatta atcggtaaca
    12901 aagtgagctg ttatgactga taaaaccatt gcgttttcgc tactcgatct ggcccccata
    12961 cccgaaggct cttcagcgcg ggaagcgttc tcccactctc tcgatctcgc ccgtctggct
    13021 gaaaagcgcg gctatcatcg ctactggctg gcagaacacc acaatatgac cggcattgcc
    13081 agtgctgcaa catcggtatt gattggctat ctggcggcga ataccaccac gctgcatctg
    13141 gggtctggcg gcgtgatgtt gcctaaccac ttaccgttgg tcattgccga acagttcggc
    13201 acactcaata cgctctatcc ggggcgaatc gatttggggc tgggtcgtgc tccgggtagt
    13261 gaccaacgga caatgatggc gctacgtcgt catatgagcg gcgatattga taatttcccc
    13321 cgcgatgtcg cggagctggt ggactggttt gacgcccgcg atcccaatcc gcatgtgcgc
    13381 ccggtaccag gctatggcga gaaaatcccc gtgtggttgt taggctccag cctttacagt
    13441 gcacaactgg cggcgcagct tggtctgcca tttgcgtttg cctcacactt cgcgccggat
    13501 atgttgttcc aggcgctgca tctttatcgc agtaacttca aaccgtcggc tcggctggaa
    13561 aaaccatacg cgatggtgtg catcaatatt atcgccgccg acagcaaccg cgacgctgaa
    13621 tttctgttta cctcaatgca gcaagccttt gtgaagctgc gccgtggcga aaccgggcaa
    13681 ctgccgccgc cgattcaaaa tatggatcag ttctggtcac cgtctgagca gtatgacgtg
    13741 cagcaggcgc tgagtatgtc gttggtaggc gataaagcga aagtgcgtca tggtttgcag
    13801 tcgatcctgc gcgaaaccga cgctgatgag attatggtta acgggcagat tttcgaccac
    13861 caggcgcgcc tgcattcgtt tgagctggcg atggatgtta aggaagagtt gttgggatag
    13921 tgtgtcttaa cgcgggaagc cttatccgag ctggcaacgc tgtcctacgt aggcctgata
    13981 agcgaagcgc atcaggcatt gtgtaggcag cagaaatgtc ggataaggca ccgctgatta
    14041 ctgatacacc ggcagtaaat taaagctcga taaaatatgc accagtgcgt tgccgacgcc
    14101 aaacaccaga atcagcgcaa tcatcggctt gccaccccag acgcggaatt tcgggctgcc
    14161 aaagcgttta cgcgatgcgc gggccagcag tgccggaaca attgccgccc agatggtagc
    14221 cgctaaacca gcataaccaa tggcatacag gaatccgttc gggaacagca gaccacccac
    14281 aacaggcggg gcaaaggtca gcaatgccgt tttcaagcgg cccacagccg agtcgtcgaa
    14341 accaaacaga tctgccagat agtcaaacaa acccagcgtt acgccaagga acgaactcgc
    14401 taccgcaaag tttgagaaca cgaccagcag aaggtccagg ctacggctgt tcagcacgcc
    14461 gcttaatgct tgtaccaaca catcaatatt accgcccttc tcggcgatgc cgataaactc
    14521 cggacgaggg atgttgccca ttgtcgccag caaccagatg gtatacagcg ccagtgccat
    14581 cagcgtaccg tacaccaggc atttcacgat ggttttcgga tctttgccgt aatacttcat
    14641 caggcttggc acgttaccgt gataaccaaa cgacgccagg cagaacggca gggtcatcag
    14701 caaatacggc gcataagatg cattgctttc ggcaacgttg aacaatgtcg caggctgcac
    14761 atgccccagc aggctaccga aggtgaggaa gaaggtaatg actttcgccc ccagcacaat
    14821 cgccgtcatg cggctgacgg ctttagtact caaccacacc acaaacgcta ccagcagtgc
    14881 aaaaccaaaa cccgccgccc gtgccgggac gtttagcgac atctctgcga aggtgtgatg
    14941 cagaatcgaa ccactggcag aaatataggc ataggtcagg atatagagca caaaggcaat
    15001 ggaaatgccg ttgaccacgt tccagccttt gcccagcaaa tctttggtga tggtgtcaaa
    15061 actcgaaccg attcggtaat tcaggttagc ttccagaatc atcaagccgg aatgcagcat
    15121 acagaaccag gtaaagatca gcgctgccat tgaccagaaa aaccacgccc cggacatgac
    15181 cactggcaga gaaaacatcc ctgcgccaat aatggtgccg ccgataatca ccacgccgcc
    15241 aagcagcgac ggtgacgttt gggtggtggt tagtgttgcc atgagggctt ctctccagtg
    15301 aaaaatagtg cgactgcgtt gttatgcatt gcactgtacc agtacatgag tacaaaagac
    15361 agaaaaaaag ccccgatggt taaaatcggg gctgtatata ttattttaca gattgtgttc
    15421 gctgttcagc gatgattacg catcaccacc gaaacgacga cgaccggtag agtcatcacg
    15481 acgcggtgcg cggccttcac gacgttcgcc gctaaaacga cgaccatcac cacgaccacc
    15541 ttcacggcgt tcaccgctga agttacgacc gccttcacga cgctcgccac cgaaaccacg
    15601 accaccgcca cgacgctcac cgccagtatg cggctgtgca tcgcccagta actgcatgtt
    15661 catcggcttg ttgagaatgc gagtgcgcgt aaagtgttgc agcacttcac ccggcatacc
    15721 tttcggcagt tcgatggtgg agtgagaagc aaacagcttg atgttaccga tgtaacggct
    15781 gctgatgtcg ccttcgttag caatcgcgcc aacgatatga cgaacttcaa caccatcatc
    15841 gcggcccact tcaatgcggt acagctgcat atcgccaaca tcacgacgtt cacgacgcgg
    15901 acgatcttca cggtcgccac gcgggccacg gtcgttacga tcgcgcggac cacggtcatc
    15961 acggtcacgg aattcacgtt tcggacgcat cggcgcatct ggcggaacaa tcagagtacg
    16021 ttcaccctgt gccattttca gcagtgccgc agccagagtt tcgagatcca gctcttcacc
    16081 ttctgcagtc ggctgaattt tgctcagcag tgcgcggtat tgatccagat cgctgctttc
    16141 cagctgctgc tgtactttag cggcgaattt ttccagacgg cgtttgccca gcagttctgc
    16201 gttcggcagt tctacttccg gaatagtcag cttcatagta cgttcaatgt tgcgcagcag
    16261 acgacgctcg cggttctcaa cgaacagcag cgcgcggcca gcacgacccg cacgaccggt
    16321 acgaccgata cggtgaacgt aagactcaga atccatcggg atatcgtagt taaccaccag
    16381 gctgatacgc tcaacgtcca ggccacgggc cgcaacgtcg gtcgcaatca ggatgtccag
    16441 acgaccgtct ttaaggcgtt ccagtgtctg ctcacgcagc gcctggttca tgtcaccgtt
    16501 cagcgcggcg ctattgtagc cgttacgctc aagagcttca gccacttcca gagtcgcgtt
    16561 tttggtacga acgaagataa tcgccgcatc aaaatcttcc gcttccagga aacgtaccag
    16621 tgcttcgttt ttacgcatac cccacacagt ccagtagctc tggctgatgt caggacgggt
    16681 agtcacgctg gactgaatac gcacttcctg cggctctttc ataaagcggc gggtaatgcg
    16741 acgaatcgct tccggcatgg ttgcagagaa cagagcggtc tgatgacctt ccgggatctg
    16801 cgccataatg gtttcaacgt cttcgatgaa gcccatgcgc agcatttcgt cagcttcatc
    16861 cagaaccaga ccgctcagtt tagagaggtc cagagtgcca cgtttcaggt ggtccagcag
    16921 acggcccgga gtaccgacaa cgatctgcgg cccctgacgc agggcgcgta attgcacgtc
    16981 ataacgctgg ccgccgtaca gggcaaccac atttacgccg cgcatgtgtt tagagaaatc
    17041 cgtcattgct tcagcaacct gtaccgccag ttcgcgggtc ggtgccagca ccagaatctg
    17101 tggtgctttc agctcaggat caagattctg cagcagaggt aaagagaatg ctgcagtttt
    17161 tccgctcccc gtctgggcca tacccagaac gtcgcggcca ttcagcagat gtggaataca
    17221 ctctgcctga attggagatg gtttttcgta acccagatcg ttaagggctt caaggatagg
    17281 agccttcagg cccagatctg caaaagtggt ttcgaattca gccatgtagt acgtgtgcct
    17341 caaaattaat ggcggccagt ctacataact catcatgaaa ttgatcagca attttcattg
    17401 aaaagtgtga accggctcaa agtaggtgta ttaacgaaca acaacgccct cacccgttaa
    17461 ggtgatggca atcaaaaaag attacgggct gatgtgtacg tcagctattg ctggtccgat
    17521 tctgccaggt catcttggtc ctggcccagg agcgataatt ccaacaatgc gtatcggtgc
    17581 tcaacaaagt tatgaacgtt gttggcaacc gccagtttga acagtgccgt ggcgctgtcc
    17641 aaatccccca gacttaggta gtacttacct aaatagaagt tggtttcact gagatgctca
    17701 gcgagcgagg tgttatccgt tgcgtccgcc ttgagccttt ccattaacgt ttgttcgcta
    17761 atgttgccca ggtagaactc gacaatgttc catccccact gttccttatc cgatttttcg
    17821 aagtgctgtt tcaatacttc tttagcctgc ttctcatcga gcttctgctc ggcgagataa
    17881 agccacagac tacggaaagg atcattggga tcgtcttgat aaaacgccag cagatcatct
    17941 tgcgctaact tgtcacgacc gccgtaatat aatgcaatcc cgcgattcaa gtgcgcgtag
    18001 ttgtaagttg gatcaagctc aagtacagaa tcaaacgctt cataggcagc atcaaaattg
    18061 cctgcctgcg ttaaatatat gcctaagtaa ttgaatactt caggcatatc cggtcggatt
    18121 gccagcgctt gcgaaaaatc gttacgcgct aatgccctca gaccgagact atcatacaac
    18181 actccgcgct catataaaag ctgtgcgcgt tcgtcatcgg ttaaagcccg actggcaagg
    18241 atttgttcca tacgtgccag aatcacttcc tgctgtaaag tcggttgcaa tggtaccgcg
    18301 aggacttcac ttttacgcca ggaagtatta ctgcatcctg caagcgtaag tgctgtcgca
    18361 acgaaacacc agcgcaaaaa aggcttcatt tcccactccc gaagaccacg gttgaatgaa
    18421 cgtcctgttc ccggttgcta acaaggcgtc ctgcccggtt aaaagccccc cgccgcagcg
    18481 gagggcaaat ggcaacctta ctcgccctgt tcagcagccg gagcttccgg tgctgcagca
    18541 ggttgagact gctcagtcgc ttctttaatg ctcagacgga tacggccctg gcgatcaact
    18601 tccagaactt tcaccggtac ttcctgaccc atctgcaggt aatcggtcac tttctcaacg
    18661 cgtttgtcag cgatttgaga gatgtgtacc agaccttctt taccgccgcc gatggcaaca
    18721 aatgcgccaa agtcaacgat acgggtcact ttaccagtgt agacgcggcc cacttcgatt
    18781 tctgcagtga tctcttcgat acgacgaata gcatgtttcg ctttctcgcc gtcggtcgct
    18841 gcaatcttca cagtaccgtc atcttcgatt tcgatggtgg tgccagtttc ttcggtcagg
    18901 gcacggataa cagagccgcc tttaccgata acgtctttga tcttgtccgg gttgatcttg
    18961 atggtatgga tacgtggtgc gaactcagag atatcgccac gcggcgcgtt gatcgcctgt
    19021 tccattacgc ccaggatatg cagacgcgca cctttagcct ggttcagcgc aacctgcatg
    19081 atctctttgg tgataccttc aattttgata tccatctgca atgcagagat accctcgcgg
    19141 gaacccgcaa ctttgaagtc catatcgccc aggtgatctt cgtcgcccaa aatgtcagac
    19201 agtacaacgt agttgtcgcc ttctttcacc agacccattg cgatacccgc aacggcagct
    19261 ttgatcggca cacctgcgtc catcagcgcc agagacgcgc cgcacacgga agccatagaa
    19321 gaggaaccgt tggattcagt gatttcagac acaacacgta cggtgtacgg gaatttgtcc
    19381 atatccggca tgactgccag cacgccgcgc ttcgccagac gaccgtgacc aatttcacga
    19441 cgcttcggag aaccgaccat gccggtttcg cctacggagt acggagggaa gttgtagtgg
    19501 aacaggaagg tatcggtacg ttcgcccatc agttcatcaa gaacctgcgc gtcacgagca
    19561 gtacccagcg ttgcggtaac cagcgcctgc gtttcaccac gggtgaacag cgcagaaccg
    19621 tgagtacgcg gcagcacgcc agtacgcaca tccagaccac ggatcatatc tttttcacga
    19681 ccgtcgatac gcggttcgcc tgccagtacg cggctacgaa caacgttttt ctcgatcgcg
    19741 tgcagaattt cacccagttc gttttcgtcc agggtttcgt cttcagcaag cagcgtcgcg
    19801 atggtttcag atttgatgac atcaacctgc gcataacgct cttgtttgtc ggtgatgcgg
    19861 taagcatcgc tcaggcgagc ttcagccagt gcagcaacgc gcgcgttcag cgcttcgttt
    19921 accggctccg gctgccagtc ccaacgtggt ttaccggctt ctttcaccag ttcattgatg
    19981 ttctgaataa caacctgctg ttgttcatga ccgaacacta ctgcgcccag catctggtct
    20041 tcgctcagca gttcagcttc agattcaacc atcagtacag cggcttcagt accggcaaca
    20101 accagatcca gtttgctctc tttcaactcg tcctgagtcg ggttcagtac gtactggtca
    20161 ttgatgtaac ctacgcgagc agcaccaatc ggaccattga acggaatacc agacagagac
    20221 agtgctgcgg aagcaccaat catcgcgacg atatccgggt taacttgcgg gttaacagaa
    20281 accacggtgg cgataacctg aacttcgttg acgaagcctt ccgggaacag cggccgaatc
    20341 gggcggtcaa tcagacgcgc gatcagggtt tcgccttcgc ttgggcggcc ttcacgacgg
    20401 aagaagctac ccgggatacg accagcagcg taggtacgct cctgatagtt aacggtcagt
    20461 gggaagaagt cctgacctgg tttggctttt ttctggccaa caacggtaac aaataccgcg
    20521 gtgtcatcca tgctaaccat aacagcggca gtagcctgac gagccatcat gccggtttcc
    20581 agagtcacgg tgtgttggcc gtactggaat ttacgaacga tcggattaag caatgtaata
    20641 tcctttctct ttcttagaca gtaccttacg gcactggtgt taatacccga tcttctgcgc
    20701 atcctcgcga ctaatgacaa ccctaaccca gctctatgtg ggtaaagcct ctcattagcc
    20761 gcgcgaacct ctgcaacgga agatcattca tagcaacaat acattagttt ccagtgaatt
    20821 gctgccgtca gcttgaaaaa aggggccact caggccccct tttctgaaac tcgcaagaat
    20881 tagcgacgca gacccaggcg ctcgatgagc tgggtgtaac gtgctacgtc tttacgtttc
    20941 aggtagtcga gcagtttacg acgctgagaa accatgcgca gcagaccacg acggctgtgg
    21001 tgatcttttt tgtgctctgc aaagtggccc tgcaggtggt tgatctgtgc agtcagcagt
    21061 gctacctgaa cttcagtaga accggtgtcg tttgcgtcac gaccaaactc agaaacgatt
    21121 ttagctgttg cttcagtact tagagacatt ttaaaactcc aaagtatata gaatgaaagg
    21181 acgccgatct ctaattcagc gatcccagtg tacgttacgc aaagtgttaa acaatttacg
    21241 cgacgttaag cggcagtatt ctactcgtag cgacctgtta tcgcaagacg gttaacatta
    21301 cgccgggtat tcaaccacca ggcgacgagg cgcaacgcgg ccttcatcgt caatttcgcc
    21361 cataccgata aatttgccgt tctcaccttc tgtaacgcga accagtcctt ccagcggcgc
    21421 accggatgta cgaactgggt taccattttt gaagtaaaca gaagacgtta acggaagatt
    21481 caccaccggg tagtccgaag ctggactgtc cattggcatc agtaatggat caagtaactc
    21541 cgcagccgga atatcctgct gttcggcttg ttcgaccagt tcacgcagat gttccagggt
    21601 cacaatccgt tcaaccggat atttacttac cgccagacga cgcaggtaaa taacatgcgc
    21661 gccacaaccg agtttttcac ccaggtcatc aatgatggtg cggatataag tgccttttga
    21721 gcagtgaatt tccagctcca gctcattgcc ttcgtggcga ataaacagca attcataaac
    21781 ggtaatcggg cgcgcttcac gcggtacttc aatgccctga cgcgcatatt cgtacagttt
    21841 tttaccctga tatttcagtg ctgaatacat cgaagggatc tgttcgatat cgccacggaa
    21901 agtatccagc gccgccgcca gttgctctgc gctaaaggtt accggacgtt cttcaacgat
    21961 ttgtccgtcg gcatcagaag tatcggtacg ctgtccaaga cgcgcaatga cccgatagcg
    22021 tttgtcggag tccagcagat actgggaaaa cttcgtcgct tccccgaggc aaattggcaa
    22081 catgccagtt gccagcgggt ccagcgcacc ggtatgcccg gcacggttgg cgttataaat
    22141 ccgtttcact ttttgcagcg catcattgct ggacataccc tgcggtttat ccagcaacaa
    22201 aacgccgtta atgtcgcgac cgcgacgacg aggacgactc attagtcctc cttgctgtcg
    22261 tccgggttaa cacgacgttc ttcgtcatgt ttgaccacgc tggtcaccag gtttgacatg
    22321 cgcatccctt caaccagaga gttgtcgtag aagaaggtca gttccggcac gatacgcagg
    22381 cgcatcgctt tccccagcag gctgcggatg aaaccagaag cttcttgcaa cgctttgatg
    22441 cccgctttaa ccgcgtcttc atctttgtcg ttgaggaacg tcacatatac tttggcatac
    22501 gccaggtcgc gagacatttc gacaccggaa acggtggtca tcatgcccag gcgaggatct
    22561 ttaatttcac gctgcaggat gagagcgatc tctttttgca tttcctgcgc tacgcgctgc
    22621 gggcgaccaa attctttcgc cataataaat tctcctgaca aaaaaggggc tgttagcccc
    22681 tttttaaaat taatttcagg tggaagggct gttcacgttg gcctgataag acgcgccagc
    22741 gtcacatcag gcaatccatg ccggatgcgg cgtaaacgcc ttatcccgca tggaacccta
    22801 aaaaccttaa gcgatggtac gttggatctc gatgatttcg aatacttcga tcacatcgcc
    22861 agtgcggacg tcgttgtagt tcttaacgcc gataccacat tccataccgt tacggacttc
    22921 gttaacgtca tctttgaagc ggcgcaggga ctccagctcg ccttcgtaga taaccacgtt
    22981 gtcacgcaga acgcggatcg ggttgtgacg tttaaccaca ccttcggtaa ccatacagcc
    23041 tgcgatggca ccaaatttcg gcgatttgaa cacgtcacga acttccgcca gaccgataat
    23101 ctgctgtttc agttccggag acagcatacc gctcatcgcc gctttcactt cgtcaatcag
    23161 gttatagatg acggagtagt aacgcagatc caggctttcc gcttcaatca ctttacgtgc
    23221 agaggcatca gcacgtacgt tgaagccaac caggatggcg ttggacgccg cagccagggt
    23281 ggcgtcggtt tcggtgatac cacctacgcc agaaccgatg atcttcactt taacttcgtc
    23341 agtagacagt ttcagcaagg agtcggagat cgcttccaca gaaccctgta cgtctgcctt
    23401 caggacgatg ttcacttcgt gaacttcgcc ttcggtcatg ttggcgaaca tgttctcgag
    23461 tttagatttc tgctgacgcg ccagtttaac ttcgcggaat ttaccctgac gatacagtgc
    23521 aacttcacgc gctttcttct cgtcacgtac aacggtaact tcatcacccg cagccggtac
    23581 gccggacagg ccgaggattt ccaccggaat ggacggaccc gcttccagca cttcctgacc
    23641 cagttcgtta cgcatcgcac gaacacgacc gtattcgaag ccacacagaa cgatatcgcc
    23701 cttgtgcaga gtaccttcac gtaccagaac ggtagcaacc ggaccacgac ctttatcgag
    23761 gaaggattcg ataaccgcac cgctcgccat acctttacgt accgctttca gctccagaac
    23821 ttccgcctgc agcaggatag cgtccagcag ttcatcgata ccagtacccg ctttcgcaga
    23881 cacatgtacg aactggcttt caccgcccca ctcttccggc aggatgccgt actgggagag
    23941 ttcgttctta acgcgatccg gatcggcttc tggtttatcg atcttgttca ctgcaacgac
    24001 caccggaacc tgcgccgctt tcgcgtgctg gattgcttcg atcgtctgcg gcattacgcc
    24061 gtcgtcggca gcaacaacca ggactacgat gtccgttgcc tgcgcaccac gagcacgcat
    24121 tgaagtaaac gcggcgtgcc ccggggtgtc caggaaggtg atcatgccgt tttcagtttc
    24181 aacgtggtat gcaccaatgt gctgggtaat gccgcccgct tcgccagagg ccactttcgt
    24241 tgaacgaatg tagtccagca gagaggtttt accgtggtca acgtgaccca tgatggtcac
    24301 aaccggcgcg cgcggttcag ccgcagcacc cgtgtcacgg tcgctcatta ccgcctcttc
    24361 cagctcgttt tcacgacgca ggataacttt atggcccatc tcttcagcaa ccagctgtgc
    24421 ggtttcctga tcgataacct ggttgatggt tgccattgcg cccagtttca tcatcgcttt
    24481 gatgacctga gagcctttaa ccgccatctt gttcgccagt tcgccaacgg tgatagtttc
    24541 gccgatcaca acgtcacggt taacggcctg agcaggcttc tggaagcctt gctgcagcga
    24601 agaaccttta cgttttccgc ctttaccgcc acgtactgct gcgcgtgctt cttcacgatc
    24661 agcttttgat tcagcgtgtt tgttgccttt cttcggacgc gctgctttcg cgttacgacc
    24721 acggccacgg ccgccttcga cttcacgatc gctttcgtct tctgcctggc gagcatgttg
    24781 agaagtagtg acgtgataat cgctggaatc ttcagtcggt tccgcgttat cagtccattt
    24841 gttttcttcc gccatacgac gtgcttcttc agcaacgcga cgtgcttctt cttcgagttt
    24901 acgacgcgct tcttcttcag ctttacgctt gagctctgca gcttcctgct cacggcgggc
    24961 tttttcagcc tgggcgtttt tagtcatatc gtcttgttga ttgctcactt tgtctttttc
    25021 cgcagcttca cgtttcgctt gttcagcagc ttcacgctta gcttgttctg cggcctcacg
    25081 ttcagctttt tgttgcgcct cgcgtttagc cgattcttct gcctcacgac gggcttgctc
    25141 ttccgctgca aggcgttcag cctcttgcgg atcgcgtttc acaaaggtgc gtttcttgcg
    25201 gacttcgatt tgtaccgatt tgctttttcc accggtacca ggaatgttaa gggtgctgcg
    25261 tgttttacgt tgcagcgtca atttgtccgg gcctgaattt ttctgattca ggtggtcaat
    25321 caaagtctgt ttctcttgtg cagacacaga gtcgtcagca gacttccgga tacctgcatc
    25381 agcaaattgc tgtaccaggc gttccacgga ggtctgtcgc tctgcggcca gcgttttaat
    25441 cgttacatct gtcatgctgt tccttcctgc tacagtttat tacgcttcgt caccgaacca
    25501 gcaaatatta cgggcagcca taatcagtgc tccggctttt tcgtcggtca acccttcgat
    25561 atcagccaga tcatcaatgc cctgttcggc gagatcttcc agcgtacaaa cgccacgggc
    25621 ggccagtttg aatgccaaat cacgatctac cccttcaagg ttcagcagat cgtcagccgg
    25681 tttgttatca ccgaggcttt cttcctgggc ctgtgcaatg gtggccagtg catttttagc
    25741 acgctcgcgc agtgcttcaa cggtcggctc atcaaggcct tcgatttcca acagctcttt
    25801 catcggcaca taggccagtt cttccagcgt cgagaaacct tcttctacca gaacagtcgc
    25861 gaagtcttcg tcgatgtcga gatatttggt gaaggtgtcg atcgctgcgt gcgcttccgc
    25921 ctgatgctta gcctgcaggt cgtcaacggt cattacgttg agttcccaac cgctcagctg
    25981 cgaagccaga cgcacgttct gaccgttacg gccaatcgcc tgcgccaggt taccagcttc
    26041 aacggcgata tccatggtgt gtttatcttc atccaccacg atagaagcaa cgtctgccgg
    26101 tgccattgcg ttaatcacaa actgcgccgg gttatcatcc cacaggacga tatcgatacg
    26161 ctcgccgccc agttcagtag aaaccgcctg aacacgcgcg ccacgcatac ctacgcaagc
    26221 acctaccgga tcgatacgtt tatcgttggt tttcaccgcg attttcgcac gagaacccgg
    26281 gtcgcgagcc gctgctttaa tttcaatcac ttcttcgccg atttccggca cttcaatacg
    26341 gaacagttcg atcagcattt ccggcttgga acgagtgacg aacagttgcg cgccacgcgc
    26401 ttccgggcga acggaataaa gcacgccacg aacgcggtcg ccagggcgga agttttcacg
    26461 cggcagcata tcttcgcgca ggatcacggc ttcagcgttg ttgcccagat ccagagagat
    26521 gttgtcgcgg tttacttttt tcaccacgcc ggtgatgatt tcaccttcgt gttcacggaa
    26581 ctgatcaacc accatcgcac gttcggcttc acgcactttc tgcacgataa cctgttttgc
    26641 cgtctgggtg gtgatacggt caaaggtaac agactcaatc tgatcttcaa cgtaatcgcc
    26701 caagttcagg ctttcatctt cataacgtgc ggcttcaagg gtgatttcct tggtcggctg
    26761 ggtgacttca tcaacaacta accagcgacg gaaggtgtca aaatcaccgc ttttgcgatc
    26821 gatctgtaca cggacgtcga tctcttgctc atattttttc tttgttgctg tcgccagcgc
    26881 gctttccaat gcttcgaaaa tcttctcgcg aggtagcgcc ttttcattgg atacggcttc
    26941 aactacagcc aaaatttctt tgttcatcgc gggcttttca cctcatccag actattaaaa
    27001 gtggggaacc aggttcgcct tctggatatt actcagcgcg aacacttcat ctttaccttc
    27061 gacggtaact gtgatcattt caccgtctac cgctttgata acgccctgcc atttacgacg
    27121 gttttgtacc gccatacgga gaaccagagt cacctcttct ccgacaaaac gggcgtagtg
    27181 ttcagccgtg aacagtgggc gatcgagacc cggtgaggag acttccaggt tataagcaac
    27241 ggtgatggga tcttcaacat ccagcacagc acttacctgg tggctcacat cagcacaatc
    27301 atcaacattg atgccatctt cactatcaat atagatgcgc agtgtggatg tgcgaccgcg
    27361 aataaattcg atgcctacca gttcaaaacc cagggcctca actggcgcag taatcatctc
    27421 tgttaatttt tgctctaatg tggacaagcc cacccccaag acataaaaaa agggcctaaa
    27481 gcccagttat tctgtagtca gataacaaaa aaccccgata aatcggggct ttatataact
    27541 gaaccctata accgcaactg cggtctggag cactttccag aaggattttt tcaaatccca
    27601 ctacgaaggc cgaagtcttc acagtatatt tgaaaaagga ctctaaggga aagtggttgc
    27661 gggggccgga tttgaaccga cgaccttcgg gttatgagcc cgacgagcta ccaggctgct
    27721 ccaccccgcg cctgaaacgt ggcaaattct actcgttttg ggtaaaaaat gcaaatactg
    27781 ctgggatttg gtgtaccgag acgggacgta aaatctgcag gcattatagt gatccatgcc
    27841 acattttgtc aacgtttatt gctaatcacg tgaatgaata tccagttcac tttcatttgt
    27901 tgaatacttt tgccttcccc tgctctccct taagcgcatt attttacaaa aaacacacta
    27961 aactcttcct gtctccgata aaagatgatt aaatgaaaac tcatttattt tgcataaaaa
    28021 ttcagtgaga gcggaaatcc aggctcatca tcagttaatt aagcagggtg ttattttatg
    28081 acgacgattc tcaagcatct cccggtaggt caacgtattg gtatcgcttt ttccggcggt
    28141 ctggacacca gtgccgcact gctgtggatg cgacaaaagg gagcggttcc ttatgcatat
    28201 actgcaaacc tgggccagcc agacgaagag gattatgatg cgatccctcg tcgtgccatg
    28261 gaatacggcg cggagaacgc acgtctgatc gactgccgca aacaactggt ggccgaaggt
    28321 attgccgcta ttcagtgtgg cgcatttcat aacaccaccg gcggcctgac ctatttcaac
    28381 acgacgccgc tgggccgcgc cgtgactggt accatgctgg ttgctgcgat gaaagaagat
    28441 ggcgtgaata tctggggtga cggtagcacc tataaaggaa acgatatcga acgtttctat
    28501 cgttatggtc tgctgaccaa tgctgaactg cagatttaca aaccgtggct tgatactgac
    28561 tttattgatg aactgggcgg ccgtcatgag atgtctgaat ttatgattgc ctgcggtttc
    28621 gactacaaaa tgtctgtcga aaaagcctac tccacagact ccaacatgct tggtgcaacg
    28681 catgaagcga aggatctgga atacctcaac tccagcgtca aaatcgtcaa cccgattatg
    28741 ggcgtgaaat tctgggatga gagcgtgaag atcccggcag aagaagtcac agtacgcttt
    28801 gagcaaggtc atccggtggc gctgaacggt aaaaccttta gcgacgacgt agaaatgatg
    28861 ctggaagcta accgcatcgg cggtcgtcac ggcctgggca tgagcgacca gattgagaac
    28921 cgtatcatcg aagcgaaaag ccgtggtatt tacgaagctc cggggatggc actgctgcac
    28981 attgcgtatg aacgtctgtt gaccggtatt cacaacgaag acaccattga gcagtatcac
    29041 gcgcatggtc gtcagttggg ccgtctgctg taccaggggc gttggtttga ctcccaggcg
    29101 ctgatgctgc gtgactctct gcaacgctgg gttgccagcc agatcactgg tgaagttacc
    29161 ctggagctgc gccgtgggaa cgattattca atcctgaata ccgtctcaga gaacctgacc
    29221 tacaagccag agcgtctgac gatggaaaaa ggcgactcgg tgttctcgcc agatgatcgt
    29281 attggtcaat tgaccatgcg taatctggat atcactgata cccgagagaa actttttggt
    29341 tatgccaaaa ctggcctgct ttcctcctct gccacttcag gcctgccgca ggtggagaat
    29401 atggaaaaca aaggccagta attcgacatc aaccctgccc ttcaggggtg gggttacctt
    29461 gcttatcctc tgtaaactcc tttctgagca tcacataatc gaaaacatca tggtttgcat
    29521 ccacaacccg tgagtctcct ttatagtgtg caatcacttc ctcaagcgtt tgcggctgtt
    29581 ccggctttgt tacccccatc cacgcattaa taaggtaatt attgtaagct gtcgaaaaga
    29641 tattgttttc cgttgtgcga tccacgccat ccccaagaac ggggctatac cagataaaca
    29701 tcgggacatg atatgcctgc tggctagcct ccctgcctcc atgaaaatag acgttcttct
    29761 tcgtagggtc acgttccagg ccgtggtcgg caaaatacat gaccgaggcg cggcgatctt
    29821 ttaataattc aaaaacctga cctagcaaac tatctgtgta atgaatggag ttgtcatagc
    29881 aggcatcctg atcgtcctgc ggttgaaaca cggcgctgga ttgcggatag gcgctacaag
    29941 ccggttcatg gcttccattt aaatgaagaa caatcagttt cttttgctgc gtattttgct
    30001 gtaatgcttg cgataaatgc ggcaacaaca attcatcaaa tcctctgaca taaactgttt
    30061 ccatggcgcg catggcgata ctggtaactg ctgtaccatt ctgccgaaaa gcggattgcg
    30121 agcttagcca gaaagtctga aatcctgctt gattagccat attaataatg ttgtcggggt
    30181 aattatgaat gtcatgactc aaaacagaat cagcagttaa agaaagggga accgacagcg
    30241 cagtgtaagg tgcgccgctt attgcttgat taaacagttt gatctgtttt ctttgtgctt
    30301 caacttgcgg tgtcgtagag cgtgtatatc catacaaaga catattgtcg acacgtacag
    30361 actcccccac aatcaacacg taggtatcaa tacttgtatc cctgactgat aattgaaaat
    30421 acggcaccgt gtttgcaatt gagagtaatc tttgatgcgc tttcgctgct aaagcaaaat
    30481 agttgagatt gaaaaacggc gtataggtag caaatcacga cgctagtata tatggactga
    30541 acgctttttt attttttgca tctttataag caaattgaca agcggaaaat aaactgcccg
    30601 aaatgacaat cagcaataat attcctgtca ctttttttgt cggcaaggaa acatcatatt
    30661 ttattattac tgccaaaaaa agaagggata aaaaagcaaa agcacataga taagggatat
    30721 acatccccag cattttcaca acttcatccg gatcactctg cagcacacta atcgcaaatc
    30781 catcattgaa agttgtacca aaggtacacc atgaatagag gctaatactc atgtctgccg
    30841 cagtcagtaa cacaaatggt gcggcaacaa gcaggcgaag cggaagggat gatatacgtt
    30901 taacaaccag gatggtcagg acgaagaaca gcaatcgctg aaccatatgc gctccggaag
    30961 aggcgactaa gttcgtaata ccaaaaacaa taacacaaag atacaacaac caatgagatt
    31021 taaaatcttc tagcaaattt gttgaatact gtcatccaga aatccaagtt cacaaccaat
    31081 ataaacaatt atcaacaatg ttcaatttta tatcagacat gtttttcaac tctgatccat
    31141 atcagaccga aacactgtta gttttttctt tttaaatgat gcgaccattg cgttctcagt
    31201 aattaaataa cattatcaat gcgtctctaa acattacgca gaacgtagcc atcagtagtt
    31261 atactgacag gagcaggatg gtttatcgag aagtaaacat atccgtattc gtaccggaac
    31321 tggagttgac acaataaagt gccaattatg tcagcagtag ggaaaagttt ataactaagg
    31381 cgtaccggca ccatcgtttc aaggtaccag ctacgagtaa agcaactgga cgagatacag
    31441 atacctgaca accattcatc agaccaggac caaaacgaaa agagacgctt ttcagcgtct
    31501 cttttctgga atttggtacc gaggacggga cttgaacccg taagccctat tgggcactac
    31561 cacctcaagg tagcgtgtct accaattcca ccacctcggc acggatacta ctttttagtt
    31621 cgggatatcg ctggtcggct tagccggagc agctggctga gtttgttcgg ttttcgccgg
    31681 tgcactcaga ttttcccatt cgctacctct attggttttg ttgctattga tgttacccag
    31741 caccagactg atgatgaaga ataacgttgc cagcagcgcc gtcatgcggg tcatgaagtt
    31801 accagaacca cttgaaccaa acagcgtagc ggaagcgcct gctccgaagg aggctcccat
    31861 atcagcgcct ttaccttgct gcagcatgat cagaccaaca aggccaattg ccacaataag
    31921 gaaaactact aaaagagctt cgtacataat caacctgttc cttgcggagt tgccgcgtac
    31981 caatgcttca accaataaag cgggagtttt taatctttcc cactgaagcg ggtgtgaata
    32041 ctaaccaaaa cgaatgacct tcgcaagggc aattttatcg cattgtatca actgcggaaa
    32101 aaaacagcaa aaccgatgtg ttcgccgaga aaaaaggcga caatcgccgc ctttttagcc
    32161 agttatctaa cgctttaaac ggcttttact gcatcggcga tgcggtgtgc aaattcagtc
    32221 acctgcgctt cgtcttcgcc ttccaccatc acgcgaatta acggttcggt gccggattta
    32281 cgcagcaaca cgcgtccacg gttgcccagc gcagcttcaa cctctgcggt cacggcttta
    32341 actgactcat gctcaagcgg atcgccgcta cctgcggtgt aacgtacgtt aaccagaatc
    32401 tgcgggaaca ttttcatgcc gctgcaaagg tcgtgcaggc tcatatggtt acgtgccatc
    32461 gccgccagca cctgcaagcc agcaacgatg ccgtcaccgg tagtggtttt atccagcagg
    32521 atcacatgac cggaattctc tgcaccgata cgccagcctt tctcctgcat tttttccagt
    32581 acgtagcggt cacccacttt cgcgcgcgca aatggaattc ctaactgttt cagcgccagt
    32641 tcaagcccca tgttgctcat caatgtaccc acagcaccac cacgcagctg gccctgacga
    32701 agaccttcac gcgcgatgat atacatgatc tgatcgccat cgactttatt gccttcatgg
    32761 tcaaccataa tcacgcgatc gccgtcgccg tcgaaggcaa taccgagatc cgctttttca
    32821 gccagcacac gagcctggag cgcgcgaacg tcggtagccc ccacttcggc attgatgttt
    32881 acaccgtttg gctcacaacc gatagcgata acgttcgccc ccagttcgcg cagcacgttc
    32941 ggcgcgatgt gataagtcgc accgtttgca caatccacca caatcttcag ttcactgagg
    33001 ctaagttcgt tcgggaacgt ggctttgcaa aactcgatat agcgacccgc ggcatcaacg
    33061 atacggctgg ctttacccag ttcagccgaa tcaacgcagc tgatctcctt ttccatttcc
    33121 gcttcgatgg cctcttctac cgcatccggc agtttagtgc cgtcgataga gaagaattta
    33181 atcccattat cgtagaacgg gttatgcgat gcagatatca caattccggc ctctgcgcgg
    33241 aaggtacgcg tcagataagc cacggccggt gttggcatcg ggccagtgaa gagtgcggaa
    33301 aggcccgctg ccgccagacc cgcttccagt gctgactcca gcatatagcc agaaatacgc
    33361 gtgtctttac caataataat cttacgggag ccgtggcgcg ccagcacttt acccgcggcc
    33421 caacccagct taagcacaaa atcaggtgtg atcggcgcat cccctacacg accacgaatc
    33481 ccatcggtac cgaaatattt acgattactc atagcgtttg ttttcctttg cagacagagt
    33541 ggcttccacc acccgcatcg cttctacggt ttctttgacg tcatgaacac gaatgatgtg
    33601 cgcgccttgc attgcggcaa tgaccgcaca ggccagacta ccgctcaggc gctcggacgg
    33661 ccccacgttc agcagctgcc caatcatcga ttttcgtgac atacccacca acagcggcag
    33721 gttgaaatgg tgaaattcag ccaggcgcgc cagtaatgaa tagttatggg agagattttt
    33781 accgaaaccg aatccggggt cgagcaacaa tttctctttt gcgatacccg cctgctcgca
    33841 acgtgctatt tgctcaataa agtagcgatt cacttctgca aagacatcgt catacttcgg
    33901 agcttcctgc atggtttttg gatttccctg catatgcatc agacaaaccg gtaaaccggt
    33961 ttctgcagcc gcctccagag cgccaggttc ggaaagggag cggatatcat taataatgtg
    34021 agcgccaact ttcgctgact cacggatgac ttctggtttg gatgtatcga ccgagatcca
    34081 gacttcgaag cgttgagcaa ttgcctcaac cacaggaata acacgttgca actcttcttc
    34141 aacgctaact tccgccgccc ctgggcgcgt ggactcgcca ccaacgtcaa tgatcgtcgc
    34201 gccagcattg atcatcagat tcgcatgttt caccgcatct atcagcgagt tatgcgtgcc
    34261 accatccgaa aaggaatcag gcgtgacgtt gaggatcccc attacgtgag gatggctaag
    34321 gtccagtgaa gtaccctggg caaagagttt cattatgtta tccctggtat gaattgataa
    34381 gaaaaaaccc cggagcacgc cccggggttt tcggtacaaa tacagtcatc tgatgcggga
    34441 acttacttgt cgcctaactg ctctgacatg gtgttacccg ggttcggcgt acgcggttca
    34501 tcaaccggac gaggagcctt tggactacca ttgtcgccag aattgttaga agcgcctggt
    34561 tcttcccagc ccgctggcgg acgtacatcg cgacgtgcca tcaggtcatc aatctgcggt
    34621 gcgtcgatag tctcatattt catgagagca tctttcatcg catgcagaat atccatattg
    34681 tcggtcagaa gctgacgcgc acgattatag ttacgctcaa tcagtgcttt cacttcctgg
    34741 tcgatgatac gtgcagtttc atcggacata tgtttcgctt tcgctacgct acggccgagg
    34801 aatacttcac cttcttcttc cgcgtacagc agtggaccca atttctcaga gaagccccac
    34861 tgagtcacca tgttacgtgc caggttggtc gcaactttaa tatcgttgga cgcaccggta
    34921 gaaacatgtt ccggcccgta gatgatctct tctgccagac gaccaccgta cagcgtagaa
    34981 atctggcttt ccagtttctg acggctggcg ctgattgcgt cgccctcagg caagaagaaa
    35041 gtcacaccca gcgcacgacc gcgtgggata atcgtcactt tatgcaccgg atcgtgttcc
    35101 ggcaccaggc gaccgataat cgcatgaccc gcttcgtggt aagccgttga ttctttctgc
    35161 gcttccgtca tcaccatgga gcgacgttcc gcacccatca tgattttgtc tttcgctttc
    35221 tcgaactcaa ccatcgacac aacgcgtttg ttgccacgag cagcgaacag tgccgcttcg
    35281 ttcaccaggt tcgccaggtc agcaccggag aaaccaggag taccacgagc aatgattgcc
    35341 gcgtcgatat cgggtgccaa tggtacgcga cgcatgtgaa ctttcaggat ctgctcacga
    35401 ccgcgaacat ctggcaagcc gaccacaacc tggcggtcga aacggccagg acgcagcagg
    35461 gccgggtcga gaacgtccgg acggttagtc gcggcgataa cgatgatacc ttcgttacct
    35521 tcgaagccat ccatctcaac cagcatctgg ttcagagtct gttcacgttc atcgtgacca
    35581 ccgcccagac cagcgccacg ctggcggcct acggcgtcga tttcatcgat aaagatgatg
    35641 cacggtgccg ctttcttcgc ctgttcgaac atgtcacgaa cacgggatgc acccacaccg
    35701 acgaacattt ctacgaagtc agaaccggag atagtaaaga acggaacttt cgcttcgcct
    35761 gcaatcgctt tcgccagcaa cgttttaccg gtacccggag gaccgaccat caggacgcct
    35821 ttcgggatct taccgccgag tttctggaag cggctcggct cgcgcagata ctctaccagt
    35881 tcagcaactt cttcttttgc ttcgtcgcag cccgcaacgt cagcaaaggt cgttttgatc
    35941 tgatcttccg tcagcatgcg cgctttgctc ttaccaaacg acatggcacc tttgccaccg
    36001 ccgccctgca tttgacgcat gaagaagatc cagacaccaa tcagcaacag catcgggaac
    36061 caggagatga agatagaagc cagcaggctt ggttcttcag gcggttcacc gacaaccttg
    36121 acgttcttgg tcaacaggtt atccagtaat ttcggatcct gaaccggaat gtaagtggta
    36181 taacggttac tatctttctt ggtaacgttg atttcacgtc cgttgatacg cgcttcacga
    36241 acctggtcgt tattgacctc ttgtaggaag gtagagtaat ccaccttacg gccattagac
    36301 tcgctgggcc caaagctctg gaatactgac atcagcacaa cggcaatgac cagccagagt
    36361 attaggtttt tcgccatgtc actcaaggga ttaacctctt attacaactg tgttaaaaac
    36421 agcgtcagga tactctatat ccagcatctt tcaaactttc gtctgaaatc tcccggttag
    36481 ggtttacgcc cggtcgctac aatatacact tctcgcgaac gtgcacgaga agagtccggc
    36541 ttacgaactt tgaccttcgt aaacagggag cgaatttccc ttagatactc atcgaaacct
    36601 tcgccctgga acaccttcac tacaaaactg ccacctggcg ctaatacatc acgacacatt
    36661 tctagcgcca gttccaccag atacatggct cgggggatat ccaccgccgg tgttccgctc
    36721 atgtttggtg ccatatcgga catgacaacc tggactttgc tgtcgccaac gcgctccagc
    36781 agtgctttca tcaccagttc atcacgaaaa tcgccctgaa gaaagtccac accaacgata
    36841 ggatccatag gtaaaagatc gcaagcgatg atgcggcctt tgccgccaat ttgggtgacc
    36901 acatattgtg accaaccacc cggagcagca ccaaggtcga caaccgtcat tcccggttta
    36961 aagagtttgt cactttgctg tatttcatca agtttaaacc aggcacggga acgtaatccc
    37021 tttttctgtg cctgttgaac atatttatcg ctaaagtgtt cctgaagcca gcggctggag
    37081 ctggcagaac gcttcttacc tgtcatttaa ctttcccatg gggataactc atcgtaacca
    37141 attgcgtaaa tttttacgcg cctatttggt gatatatggg agatggcggt agaatgaccc
    37201 gttttcaatc ccaacgtaag caaaaaatac gatgaatctg agtactaaac aaaaacagca
    37261 cctgaaaggt ctggcacatc cgctcaagcc agttgttctg cttggcagta atggtttgac
    37321 cgaaggggtg ctggccgaga ttgaacaagc gttagagcac catgaactca tcaaggtgaa
    37381 aatcgccacc gaagatcgcg aaacaaaaac cttgatcgtg gaagctatcg tgcgcgaaac
    37441 cggcgcctgt aatgtacagg tcatcggtaa aacgctggtg ctttatcgcc caactaaaga
    37501 acgtaaaatc tcgctgccac gctaagatta tcctaaagtt acacacattt gctgtgtaaa
    37561 acgaggggtt ttccgcaggc aggagagcaa aatgccacgc tctgttcgtt gataaaaggc
    37621 cgcagagcgg cctttttcct ttctttacaa tacatcaaca tcttgagtat tgggtaattc
    37681 ttacaggtat tccaccttaa ttacttcaaa ttctacttcg ccgcccggcg ttttgatgac
    37741 cacaacatca tcttcttctt tgccgatcag gccacgagca ataggcgagt ttacagaaat
    37801 caggttttgt ttaaagtcag cttcgtcatc gccaacgatg cgataagtct gttcttcgtc
    37861 agaatccaga ttcagcaccg ttacggtagc accaaaaata acgcgcccat tgttgggcat
    37921 tttggtgaca tcaatcacct gcgcgtttga cagcttggct tcgatgtctt tgatacggcc
    37981 ttcgcagaag ccctgctgtt cacgagctgc gtggtattcg gcgttttctt tcaggtcgcc
    38041 atgctcacgc gcttccgcga tagcagcaat gatttcagga cggcgcacag atttcagaaa
    38101 atccagctct tcgcgtaatt tttcagcgcc gcgtaaggtc atcggaatag cttgcatttg
    38161 ttatacctct tgaatattcc tgatggggca agtcttcacc caccccggct gttcggcctg
    38221 cccggcataa ctcctcaccg ggaccagaag caaaaaaata ccgacccggg tacaagtccc
    38281 aggtcagcta caattcacat tttgatggtc attttaccct gaagttcccg aagggtcatc
    38341 gtttacttta tagggcgttg cgccgtagta tgacggctcg attccaggtt gttagcgcga
    38401 gattatgcga ttttccagat ttatcatcgg attgaccagc tgtatagcgt tcagtgttca
    38461 ggccgcgaat gttgatgagt acgttactca actccccgca ggtgccaacc ttgccctgat
    38521 ggtgcaaaaa gtcggtgcgt cggcccccgc tattgattac cacagtcagc agatggcgct
    38581 gcccgccagt acccagaaag tgattactgc gctggcggcg ttgattcaac tcggccccga
    38641 ttttcgtttc accacgacgc ttgaaaccaa aggcaatgtg gaaaacggcg tacttaaggg
    38701 tgacttagtg gcgcgatttg gtgccgatcc gacgttaaaa cgtcaggata ttcgcaatat
    38761 ggtcgcgact ttgaaaaaat ctggcgtcaa ccaaatcgat ggcaatgtgt tgatagatac
    38821 ctccattttc gccagccacg ataaagcccc cggctggcca tggaatgaca tgacacaatg
    38881 ctttagcgct ccgcctgctg ccgccatagt tgaccgcaac tgtttctccg tctcgctcta
    38941 cagtgcccca aagcctggtg atatggcttt tatacgcgtg gcatcttatt accccgttac
    39001 gatgttcagc caggtacgca ccctcccccg tggttctgcc gaagcgcaat actgcgaact
    39061 ggatgtggtg ccaagcgacc tgaaccgctt tacgctgacg ggatgcctgc cacaacgttc
    39121 tgagccgctc ccgttggctt ttgccgtgca ggatggagcc agctatgccg gtgcaattct
    39181 gaaagatgag ttaaaacagg cgggtatcac ctggagcgga acactgctgc gccagactca
    39241 ggttaacgaa cctggaacgg tagttgccag taaacagtcg gccccgctgc acgatctgct
    39301 taagattatg ctgaaaaagt cggacaacat gatcgccgat acggttttcc gcatgatcgg
    39361 ccatgcgcgc ttcaatgtgc ctggaacatg gcgggctggg tcggacgccg tgcgtcagat
    39421 cctgcgccag caagccggtg tcgatattgg taacaccatt attgccgatg gttcagggct
    39481 ttcgcggcat aacctgattg cccccgccac catgatgcag gtgctgcaat acattgccca
    39541 acacgacaat gaacttaact ttatctccat gctgccgctt gcgggctatg acggctcttt
    39601 gcagtaccgt gcaggtctgc atcaggcggg cgtggatggc aaagtctcag cgaaaaccgg
    39661 ttcgttgcag ggggtatata acctggcggg attcattacc acagcgagcg ggcaacgaat
    39721 ggcatttgta caatatcttt ctggctatgc agtaaaaccc gcggatcagc gtaatcgccg
    39781 tattccgtta gtgcgttttg aaagccgttt gtacaaagat atttatcaga acaattagtc
    39841 aaaaagaaac cccggcgcat ggccggggtt tcagattatt gacaaagtgc gctttgttta
    39901 tgccggatgc ggcgtgaacg ccttatccgg cctacaaaat cgtgcaaatt caatatattg
    39961 caatcatctt gtaggcctga taagtgtagc gcatcaggct tatttggcat tggtcattag
    40021 tgattaacgc ttgtaaatga actcaacgcc ttcttcgtcg tcttcgtccc agtcgtcatc
    40081 ccagtcttca tcatcctctt cagcaatctc ttcaagctgc tgacggtgat aatcatccca
    40141 catgaattcg actttctctg gctgtttcgc ttcttcagcc tgcacgaccg ggttttcaat
    40201 gataaaggtc atcacatccc agcagagatc tttcacgccc agtccgctcg ccgcagagat
    40261 cagataatat ttatcttccc agcccagcgc ttcagcgatc gctttcgctt tctcttcggc
    40321 ttctgcctta tccagcagat cgatcttgtt gaagactaac caacgcggtt tcgccgccag
    40381 atcctggctg tatttttcca gctcgctgat aataatacgc gcgttttcaa ccggatcggt
    40441 gccgtcaatc ggatcgatat cgatgaggtg caacaggacg cggcaacgtt ccaggtgctt
    40501 caggaagcga atgcccagac ctgcgccttc cgcagcgcct tcaatcagtc ctggaatatc
    40561 ggcaacaacg aagctctttt cgttgtccat tcgtaccaca cccagacttg gcaccagagt
    40621 ggtaaacgga taatccgcca ctttcggttt agccgccgat accgcacgaa taaaggtcga
    40681 tttacccgcg tttggcatcc ccaacatacc gacgtcagcc agcagcatca gctccagcag
    40741 cagctcacgc ttatcgcccg gcgtaccgtt agttttctgc cgtggtgtac ggttaactga
    40801 cgatttgaaa cgggtattgc ccagaccgtg ccagccgccc ttagcaacca gcagacgctg
    40861 accgtgttta gtcatatcac ccatggtttc accagtaccc tggtcgatta cacgcgtacc
    40921 taccggcact ttaatcgtca cgtctttacc gcgcttaccg gtacaatcgc ggcttgcgcc
    40981 gttctgaccg cgctctgcac ggaaagattt ttcaaaacga taatcgataa gcgtgttcag
    41041 gttctcgtcg gcttccatcc atacgtcacc accatcaccg ccgtcgccgc catccgggcc
    41101 gcctttcgga atatactttt cgcggcggaa gctcacgcaa ccattaccgc catcacctgc
    41161 aacgaccaga atcgatgctt catcaacaaa cttcatttta ttctccgtaa atcattcgcc
    41221 tgcgcggggt tgcgatacca ccgttgtatg cttacgtaat ccgccccaaa tacgatgacc
    41281 aatggcggaa tacatcgcgc ccgcaaccac gacaaacgca ccgagataac ctaaaaggtt
    41341 taacatcggt ctggcgaaga aatcgggcca ggccagtgat aaaagatctg aaaaaaacag
    41401 cgtaaacagt ggggtgagcg tgatgatcgc gctcacctgc gctgcctgcc agcgagccat
    41461 cgcttccgcc aaggcgccat atcctaccaa ggtattcagt ccgcaaaaaa ttaaacatgc
    41521 gagctgccag tggctaagct gcgctatcac tccaggcttt gccagaggga agagcgcgat
    41581 tgtacataaa gtgtacagta aaaacaggat ctgcggtgag gccagccgac gcaataaaac
    41641 cttttgcgcc acgccataac tcacccaaac cgtcgccgca ccgaccccaa agataactcc
    41701 ccaggtgtaa tcggtgagct ttgtaaatat ctcgaccaga ctggtgttaa aaaacatcac
    41761 cagaccgctc aggagcatca atgcccctac aacctgagtg ctgcgcattt tctctttcag
    41821 gataaatacg ctggcaacca tcatgccaac tggcgagagt tgcccaatca cctgcgaagc
    41881 ggtcggactc aggtattgca aggatgagct gaacaggatg aagttcccaa acagcccggc
    41941 ggtcgccact gccaacaaaa tcaaccagcg tggcttacga aacacgcgta atggcggcaa
    42001 cctcttcttc accgcaagaa tggcaccaag gccaatactc gccatcaaga aacggtaaaa
    42061 cacgattgtc ggaggttcca tcacctccag cacctgcttc attgcgattg gcaacgcccc
    42121 ccagcaaatt gctgtggtaa gcgccaaaag aatgccaatg cctgcctgct gcttcatacc
    42181 gtattccctg caaagcgcat ttgcggatta acgtcgcacc atgcgcattt ttaccggtta
    42241 tcgaatgtaa aaagccccgc aacgtgttgc ggggctttca tccgttaccg ggacgcgaaa
    42301 aacttattca gcttcgatgc tgataaattt acggtttttc gggcctttaa cttcgaattt
    42361 cactttaccg tctgctttag caaacagagt gtggtcacga ccgcaaccta cgttagcgcc
    42421 agcgtggaat ttggtaccac gttgacgaac gatgatgcta cccgccagaa cggattcgcc
    42481 accgaaacgc ttaacgccca agcgtttagc ttctgaatcg cgaccgttac gtgtggagcc
    42541 gccagccttt ttatgtgcca tttgaaatct ctcctcaggt cttaggcgct gatgccagta
    42601 attttcacat cagtgaacca ctgacgatgg ccctgctgct tacgatagtg tttacgacga
    42661 cgaaacttaa cgattttaac tttctcgcca cgaccgtgag caacaacttc agctttgatt
    42721 acgccgccat caacgaaagg aacgccgatt ttgacttctt caccgtttgc gatcatcagc
    42781 acttcagcga actcaacagt ttcgccagtt gcgatgtcca gcttttccag gcgaacggtc
    42841 tgaccttcgc ttactcggtg ttgtttacca ccactttgga aaaccgcgta catataaaac
    42901 tccgcttccg cgcacacctt ttcaatgatt cagagtgcgc tataaatatt cacaataggg
    42961 cgcgaatatt acgcaaaacg cacgcctttg acaagtgcta cagtcaatac acgaagaaaa
    43021 aaacacaact tgtacggtaa cgtttatctg tgccattttt tcagtacaat taccctatat
    43081 tcctaaccat aaaccctaag ttgcctttgt tcacagtaag gtaatcgggg cgaaaagccc
    43141 ggcttttgcg atgaatttag aaaaaatcaa tgagttaacc gcgcaagata tggcgggtgt
    43201 taatgcggca atccttgagc agcttaattc cgacgtccaa ctgatcaatc agttaggcta
    43261 ttacatcgtc agcggcggcg gtaaacgtat tcgtccgatg attgctgtac tggctgcacg
    43321 agctgttggc tatgagggaa atgcgcatgt caccattgct gccctgatcg agtttatcca
    43381 cacggcgact ctgctacacg acgacgttgt ggatgaatca gatatgcgca ggggtaaagc
    43441 taccgccaac gccgcatttg gcaatgccgc cagcgtgctg gtaggcgatt ttatttatac
    43501 ccgcgcgttc cagatgatga ccagcctcgg ttcactcaaa gtgctggaag tcatgtcaga
    43561 agctgtaaac gtcatcgcag aaggtgaagt tctgcaactg atgaacgtta acgatccgga
    43621 catcactgaa gaaaactaca tgcgcgttat ctatagcaaa accgcgcgtc tgtttgaggc
    43681 tgccgcgcag tgttccggga ttctggctgg ctgtacgccg gaggaggaga aaggcctgca
    43741 ggattatggg cgctatctcg gcactgcttt ccagttgatc gacgatttac tcgattacaa
    43801 tgccgatggc gaacagttag gtaaaaacgt cggcgacgat ctgaacgaag gtaaaccgac
    43861 gctgccgctg ctgcatgcga tgcatcatgg cacaccagaa caggcacaga tgatccgtac
    43921 cgccatcgaa caaggtaacg gtcgccatct tctggaaccg gttctggaag caatgaacgc
    43981 ttgtggatct cttgaatgga cgcgtcagcg cgccgaggaa gaagcagaca aagccatcgc
    44041 agcgttacag gtgctcccgg acaccccttg gcgagaagca ctcatcggcc tcgcgcacat
    44101 cgctgttcaa cgcgatcgtt aatcccctcc cttcatcccg cgcagtgcgc gggatgattc
    44161 cagtaaattc cataaaaaca ctaattcagc gctataacac ctgaaaaaag ggcgatgaat
    44221 ctttatatat tctgaatatt cacactcttt acaggaactt tttagcgcaa tagaccatca
    44281 ggagtatagt gatgcccgac agaagaagtg ttctgaatga aagcgactaa cttaaggagt
    44341 gaggaaagat ggaaagtaat ttcattgact ggcatcccgc tgacatcatt gcaggtttgc
    44401 gcaagaaagg aacgtcaatg gcggcggaat ctcgcagaaa tggtttgagt tcctcaacgc
    44461 tggcaaacgc attatcgcgc ccatggccga aaggagagat gattattgcg aaagccctgg
    44521 gaactgaccc ctgggttatc tggccatcac gctaccatga tccgcagacc catgagttta
    44581 tcgacagaac gcagttgatg cgtagctaca ctaaaccgaa aaaatgagtg gtctggcggt
    44641 agccccgcga acggggctgc cagctctcag acgattactc gcctttcaca cgctcaatat
    44701 ttgcacctaa agcgcgcagt ttgtcttcaa tgcgttcgta gccacgatcg atgtgataaa
    44761 tacgatcaac caccgtcgtc ccttccgcaa tacagccagc cagcaccagg cttgctgatg
    44821 cacgcagatc ggttgccata acctgtgcac cagaaagttt ttcaacacca tgacaaataa
    44881 cggtattgct ttcgatttcg gcgtgcgcgc ccatacggct cagctctggc acatgcataa
    44941 agcggttttc aaagaccgtt tcggtgatga acccggtccc ttcagccacc aggttcaaca
    45001 gcgtgaactg ggcctgcata tcggtcggga atgccggatg cggcgcggta cgtacgttaa
    45061 cagccttcgg acgtttgcca tgcatatcca ggctaatcca gtcttcgccg acttcgatgt
    45121 ccgctccagc atcacgcagt ttcgccagca cggcgtccag agtatctggc tgcgcgttac
    45181 ggcagataat tttgccacga gagatcgccg ccgccaccag gaaagtaccg gtttcgatac
    45241 gatccggcag cacgcgatag acaccgccgc ctaaacgttc cacaccttcg atgacgatac
    45301 gatcggtgcc ctgaccgcta attttcgcac ccagcgtaat caggaagttc gcggtatcga
    45361 cgatttccgg ttcacgcgct gcgttttcaa taatcgtggt gccttccgcc agggttgcag
    45421 cacacatgat ggtcaccgtt gcgccaacgc tgactttatc catcacgata tgtgcgcctt
    45481 tcagacgacc atcgacggaa gctttaacgt aaccttcttc cagtttgatg gtcgcgccta
    45541 attgttcgag gccagaaatg tgtagatcaa ccggacgcgc accgatcgta caaccgccag
    45601 gtagtgaaac ttgcccctga ccaaagcgcg ctaccagcgg ccccagcgcc cagatagaag
    45661 cacgcatggt tttaaccaga tcgtaaggtg cgcagaatac attaacgtcg cgggcatcaa
    45721 tatgcacaga accattacgt tctactttcg cacccagctg gcttagcagt ttcattgatg
    45781 tatcgacgtc tttcagtttc gggacgttct ggatctctac cggttcttcc gccagtagtg
    45841 cggcaaaaag aataggcaga gcagcatttt tagcgccgga aattgtgact tcgccctgga
    45901 gcttcgttgg cccctgaaca cgaaatttat ccatttagtt tgttctcagt taacaattca
    45961 tatccgctac cggcgaatcg cccatagctc aaaagccgtt cagtttgcga tcgcgcgccc
    46021 actccgcagg ggtatacgct ttgatcgaca cagcatgaat gcggttatcc gcaatatatt
    46081 ccatcagcgg gccatagacc gtctgctgtt ttttaacccg actcataccg tcaaacaact
    46141 cacccacggc aataacctga aagtggctgc catcgccgga aacgtggact tcctggaggg
    46201 agagagcgtt catcaacacg ctctgaattt cattattttc catgggatct tcaatcatca
    46261 gttaataaac cagcgaaaca tcttagagca aagttgcgct ggcataaata agcaaaaagc
    46321 ctcgctgata aatcagacaa ggctcgactt gcaggcaggt ttgccggaca ggcggttaac
    46381 gccatatccg gcctgaaaaa atttaacgag gcagaacatc agcaggcaaa ttatacaatt
    46441 tcgcgagggt atacactttg tcgtttaccc cctgaagcgt cacgttgttg ccctgctttt
    46501 tcgccagatc gataagatgg agcagcagtg ccagtccccc cgtatccacg cgggacacac
    46561 ggctaagatc gatgcaggta atccccttca ccgcctcctc acgcatttcc caaagcggta
    46621 gcagaacgtc ctgatccagc tctccggata acgccagcgt gtcacccgtc tgcatccagc
    46681 tcagtgactc gctcattatt ttttctcttc cagagtgatt ttctgttgag aaatcgattt
    46741 cagttgcgca gtcaggccgt cgataccttt ggtacgcagc agcgttcccc actcgttttg
    46801 tttggtggtg atcatactga cgccttcagc aatcatgtcg taagcttgcc agtttcccgt
    46861 ctgggagttt ttacgccact ggaagtccag acgcaccggc ggacggccat tcgggtcaat
    46921 aatggtaacg cgaataggca caatggtttt atcgcccagc ggctgttctg gcgcaatctg
    46981 ataggtttga ccgtgataca tcgccagcgc ctgaccgtaa gcctgcttca ggtactcacg
    47041 gaaagcggca aagtaggctt cacgttgagc aggggtcgca ctcttgtaat actggcccag
    47101 caccagcgca ccggcgtatt tcacctgtac gtatggcagc agttcctgat caacaatggt
    47161 acgcagataa tccgggttgg cccgaatttg cggttgctca ttcttcaggc gatcgaacgt
    47221 tttctgcgcc gcctcgtcca tcagcttata aggattggtc tggtctgccg cggttgccgc
    47281 actcagaggt gcaatcacca gcaaagcgac catcattaaa cgtttaaaca tgcgtcggtt
    47341 ctcctgaaat tatttcgttg tacccacagg ttcagtggtt tcattattac ctggcgcagc
    47401 agctggcgca tcgccactat tcttattgtc atcgccttta ctaccgtaaa ggaactgacc
    47461 aatgagatct tccagcacca tcgcagactt ggtgtcctga attgtatcgc catccttcag
    47521 gatagcagtc cccagttccg ggtcttcaaa accgacgttt aatgccagat attgttctcc
    47581 cagcaggccg gaagtacgaa tgctcagcga actggtatca ggaatgtggt tataacgttg
    47641 ttcaatttcc agcgttacgc gcggcagata ggttttcggg tccagcgtaa tatccgccac
    47701 ccggcccaca acaacaccac caatgctgac cggagagcgc gctttcaggc cgccaatgtt
    47761 atcgaacgtc gcataaagcg tgtaggtcga ttcagtacgt atggacgtca cgttcgccgc
    47821 cttcaggcaa acaaacagcg ccgccagcag tgctgctaat aaaaagatac ccacccaaat
    47881 ttcatttttt ttcgtttgca tgaactcaat tcccaaacat caatgcggtc agcacaaaat
    47941 ccagccccag aacagccaga gacgagtgga caacggtgcg agtggttgcc cggctaatcc
    48001 cggcagacgt cgggatggcg tcgtagccgt taaacaacga aatccacgtc accgtgatgg
    48061 cgaacaccac gctcttaatc agacagttga ccagatccat acgccagtcg acggcatttt
    48121 gcattgccga ccagaagaac ccgctatcaa tgcctttcca actgacgccg accagcgatc
    48181 cgccccagat ccccacggcg acgaagataa ccgtcaacag tggtaatgaa ataaccccag
    48241 cccagaaacg gggagaaata acccgacgca gcggatccac cgccatcatc tccatactgg
    48301 agagttgctc tgtagcgcgc atcaggccga tttctgcggt taacgccgaa ccagcacgcc
    48361 cggcaaacaa cagcgcggca accaccggcc ccagttcacg cagtagcgat aacgccacca
    48421 gcatacccag actggtttcc gcgctataag tggtcagaac cagataacct tgcagcccca
    48481 acaccattcc gatgaacacg ccagaaacta caataatcag catcgacagg acgccgacat
    48541 tatagagctg gcgcaccagc agcggcgcat gtttgcgaaa ttccggtttg ccgaccagcg
    48601 cattgaataa cattaacccg gcccgcccga acgttctcag ggttttaatc cctttatgtc
    48661 cgagcgacgc cagcgcattt aacagcatga gtggcttaac tccctggtaa aagatcagcg
    48721 tgataatcgc cggcaggata gcggaacgga acaggcccgt cagctatccc gtccagaaac
    48781 tgacgtacgc gcggatcagg attcgtctgc aacgcctggg cactgccatg agcgacaatt
    48841 tttttgtccg ccaggatcca ggcgtgatcc gcaatactta acacttccgg cacatcgtga
    48901 gaaaccacca cacaagtcac gcccagcgcg ctgttcagct cagaaatcag cttcaccagt
    48961 acgcccatgg tgatgggatc ttgcccaaca aaaggttcat caaacatgat gagatccggc
    49021 tccagcgcaa tcgcacgcgc cagcgctgca cgccgcgcca tcccaccgga aagttcagaa
    49081 ggcattagtt tagccgctcc acgcagcccc acggcctcca gcttcatcat caccgtacta
    49141 tgcaacaatg gcgcgggaag ttgggtatgt tcgcgcagtg gataggcgac gttgtcaaat
    49201 acgttcatat cagtgaacaa cgccccggac tgaaataaca tgctcatccg tttacgcact
    49261 gtatacaggc gcgaacgaga catcgccgga atattctcac catcgaaaag gatctcacca
    49321 tgatctggtg cgatttgccc gccaatcaga cggagtagcg tcgttttacc gatgcccgat
    49381 ggccccatga tcgccgtgat cttccctcgc ggcacggtca gggaaatatt atcgaagatg
    49441 cagcgattgc cacgcgtaaa actgacatcg cgcatatcga ctaaattcgc cacagactgc
    49501 tccataattc acccttcgtc ttgcgttgat tttctaagca tggcgctcaa tttaaccttg
    49561 aacccaacat atttacagaa tattacccgc cgtggttagc gaaagctggc atttgtttta
    49621 ctttttagcc gcataaagtc aaaattaaga atccgttacg gctttctgaa aatcttcagc
    49681 ggaccggcga gtatacctga agaaaggacg ttagatgctt ttagctacgg cactgttaat
    49741 tgttggttta cttttggtcg tttacagtgc cgaccgcctg gtttttgccg cgtctattct
    49801 ttgccgaacc tttggcatcc cgccgctgat catcggcatg acggtggtca gtattggtac
    49861 atcgttacca gaaatcatcg tctcgcttgc cgcgtctctg cacgaacaac gcgatttagc
    49921 cgttggtaca gccctcggct caaacattat caatatattg ctgatcctcg gtctggctgc
    49981 gctggttcgt ccttttaccg tccattccga tgttctgcgc cgtgaattac ccttaatgtt
    50041 gttggtcagc gtggtggccg gttccgtact ctatgacgga caacttagtc gaagcgatgg
    50101 tatctttctc ctctttctgg ctgtgctatg gctgctgttc attgttaaac ttgcacgtca
    50161 ggggactgac agcctgacca gagagcagct tgcagagctg ccgcgtgacg gcggattgcc
    50221 cgtcgcgttt ttatggctcg gcattgcgct tatcatcatg ccagtggcca cgcggatggt
    50281 ggttgataac gccacggtgc tggcgaatta ctttgccatc agcgagttga cgatggctct
    50341 gacggcaatt gctatcggaa ccagtctgcc ggaactggca accgcaatag cgggggttcg
    50401 caaaggtgaa aacgacattg ctgtcggaaa tatcattggc gcaaacattt ttaatattgt
    50461 catcgtgttg ggtttacccg cgctgataac gccaggagag attgatccac tggcgtacag
    50521 tcgtgactac agcgtgatgt tgctggtgag cattattttt gcgttgctgt gctggcggcg
    50581 ctccccgcaa ccgggccgtg gtgtaggggt attattaact ggcggattta tcgtatggct
    50641 ggcgatgttg tactggttat cgccaatact cgttgaataa ctggaaacgc attatgtcgc
    50701 acgtagagtt acaaccgggt tttgactttc agcaagcagg taaagaagtc ctggcgattg
    50761 aacgtgaatg cctggcggag cttgatcaat acatcaatca gaatttcacg cttgcctgtg
    50821 aaaagatgtt ctggtgtaaa gggaaagttg tcgtcatggg gatggggaaa tcggggcaca
    50881 tcgggcgcaa aatggccgca acgtttgcca gcaccggtac accttcattt ttcgtccatc
    50941 ctagtgaagc cgcgcatggt gatttaggca tggtcacccc acaggatgtg gtgattgcta
    51001 tctctaactc aggtgaatcc agcgaaatca cggccttaat tccagtgctt aagcgtcttc
    51061 acgtaccgtt aatctgcatc accggtcgcc cggagagcag catggcgcgc gccgcagatg
    51121 tgcatctgtg tgttaaagta gcgaaagaag cctgtccgtt agggctggca ccgaccagca
    51181 gcaccaccgc cacgctggtt atgggcgatg ccctcgctgt cgcgctgtta aaagcacgcg
    51241 gctttactgc tgaagatttt gcgctctcac acccaggcgg cgcactgggt cgtaaacttc
    51301 tgctgcgcgt aaacgatatt atgcatacgg gcgatgagat cccgcatgtt aagaaaacgg
    51361 ccagtctgcg tgacgcattg ctggaagtta cccgcaaaaa tcttggtatg actgtcattt
    51421 gcgatgacaa tatgatgatt gaaggcatct ttaccgacgg tgatttacgc cgtgtcttcg
    51481 atatgggcgt ggatgttcgt cagttaagta ttgccgatgt gatgacgccg gggggaatac
    51541 gtgtgcgccc tggcattctg gccgttgagg cactgaactt aatgcagtcc cgccatatca
    51601 cctccgtgat ggttgccgat ggcgaccatt tactcggtgt gttacatatg catgatttac
    51661 tgcgtgcagg cgtagtgtaa agattcaagg ataaacaaca atgagcaaag caggtgcgtc
    51721 gcttgcgacc tgttacggcc ctgtcagcgc cgacgttatg gcaaaagcag agaacattcg
    51781 tctgctgatc ctcgacgttg atggcgtact gtcagatggc ctgatttata tgggcaataa
    51841 cggcgaagag ctgaaagcgt tcaatgttcg tgacggttat ggcattcgtt gtgcgctcac
    51901 ctctgatatt gaagtcgcta tcattaccgg gcgaaaggct aaactggtag aagatcgttg
    51961 tgccacattg gggatcactc acttgtatca ggggcagtca aacaaactga tcgcctttag
    52021 cgatttgctg gaaaaattgg cgattgcccc ggaaaatgtg gcttatgttg gcgatgatct
    52081 catcgactgg ccggtaatgg aaaaagtggg tttaagcgtc gccgtggccg atgcgcatcc
    52141 actgttgatc ccgcgcgccg attacgtgac gcgcattgct ggcggtcgtg gcgcagtgcg
    52201 cgaagtttgc gacttattac tcctggcgca gggcaaactg gatgaagcca aagggcaatc
    52261 gatatgagta aagccagacg ttgggttatc attgtgctat cactggcggt tctggtgatg
    52321 atcggcatta atatggccga aaaagacgat accgcccagg tggtcgtcaa caacaatgat
    52381 cccacctata aaagcgagca tacggacacg ctcgtctata acccagaagg ggcactaagc
    52441 tatcgattga ttgctcaaca cgttgaatat tattccgatc aggccgtttc gtggtttacg
    52501 cagccggtac ttaccacgtt tgataaggat aaaatcccga catggtccgt aaaagcagat
    52561 aaagccaagc tgaccaatga ccggatgctc tatttatatg gacacgttga agtcaacgca
    52621 cttgtgccag actctcaact tcgcagaatc acgacggata acgcgcagat caatctggtg
    52681 acgcaggatg ttacctctga agacctcgtc acgttatacg gaacaacatt taactccagc
    52741 ggtctgaaaa tgcgcggcaa cttacgcagc aagaacgccg agctgattga aaaggttaga
    52801 acatcctatg aaattcaaaa caaacaaact cagccttaat cttgtgcttg ccagctcact
    52861 tctggccgcc agcattccgg catttgccgt aaccggagac actgatcagc cgatccacat
    52921 tgaatcggac cagcaatctc ttgatatgca aggcaacgtg gttaccttta ccggtaatgt
    52981 catcgtcacc cagggcacca tcaaaattaa tgccgacaaa gtggtcgtta cccgtccggg
    53041 cggcgaacaa ggtaaagaag tgattgacgg atacggtaaa ccggcaacgt tctaccagat
    53101 gcaggacaac ggtaaacccg ttgaaggtca cgcttcccag atgcactacg aactggcaaa
    53161 agattttgtc gttctgacgg gtaatgctta tctgcagcag gtcgatagca acattaaggg
    53221 cgataagatc acttacctgg tgaaagagca gaaaatgcag gctttcagcg acaaaggcaa
    53281 gcgcgtaacc accgttctgg tgccgtcgca gctgcaggac aaaaacaaca aaggccagac
    53341 cccggcacag aagaagggta attaattcgt tatggcaaca ttaactgcaa agaaccttgc
    53401 aaaagcctat aaaggccgtc gcgtggtaga agacgtcagc ctgaccgtca actccgggga
    53461 aattgtcggt ctgctggggc caaacggtgc cggtaagacc accactttct acatggttgt
    53521 aggcattgtg ccgcgcgatg cgggcaacat cattattgat gatgacgata tcagtctgct
    53581 gcctctgcat gcacgcgcgc gccgcggtat cggctatctg ccacaggaag cctccatttt
    53641 ccgtcgcctc agcgtttacg ataacctgat ggcggtactg caaattcgtg acgacttgtc
    53701 tgctgaacaa cgtgaagacc gcgcgaacga gctgatggaa gagtttcaca ttgagcacct
    53761 gcgtgacagc atggggcagt cactctccgg gggtgaacgt cgccgtgtag aaattgcccg
    53821 cgcactggct gcgaatccga aatttattct gctcgacgaa ccgtttgccg gggttgaccc
    53881 gatctcggtt atcgacatta aacgcatcat tgaacacctg cgcgacagcg gcctgggcgt
    53941 gctgatcact gaccacaacg tgcgtgaaac actggcggtt tgtgaacgcg cttatatcgt
    54001 cagtcagggg catttgatcg cccacggcac gcctacagaa atcttacaag acgaacacgt
    54061 taagcgtgta taccttgggg aagacttcag actctgatag ggtagaagtt tgcgacgttt
    54121 tagcaggaga gtacgattct gaacatgaag caaggtttgc aactcaggct tagccaacaa
    54181 ctggcgatga cgccacagct ccaacaggca attcgtctgt tgcagttgtc gacgctggaa
    54241 cttcagcagg agctacagca ggcgctggag agtaatccgc tgcttgagca aatcgacact
    54301 catgaagaaa tcgacacccg cgaaacgcaa gacagtgaaa cgctggacac cgccgacgcg
    54361 ctcgaacaaa aagagatgcc ggaagagctg ccgctcgatg ccagttggga caccatttat
    54421 accgctggta caccatccgg caccagcggt gactacattg acgacgagct gccggtctac
    54481 cagggcgaaa cgacgcagac cttgcaggat tacctgatgt ggcaggtcga gctgacaccg
    54541 ttttccgaca ctgaccgcgc tattgctacc tctatcgtcg atgccgttga tgaaaccggt
    54601 tatctgactg tcccgctgga agatattctc gaaagtatgg gcgatgaaga gatcgacatc
    54661 gacgaggttg aagccgtcct taagcggatc caacggtttg acccggtcgg tgtagcggca
    54721 aaagatctgc gtgactgcct gctaatccaa ctctcccaat tcgataagac cacgccgtgg
    54781 ctggaagagg ccagattgat cattagcgat catctcgatc tgttagccaa tcacgacttc
    54841 cgcactttaa tgcgcgtcac gcgtctgaaa gaagatgtgc tgaaagaagc cgtcaatctg
    54901 atccagtcgc tcgatccgcg ccccgggcag tcgatccaga ctggcgaacc tgagtatgtc
    54961 attccagatg tgctggtgcg taagcataac ggtcactgga cggtagaact caacagtgac
    55021 agcattccgc gtctgcaaat caaccagcac tacgcctcga tgtgcaataa cgcgcgcaac
    55081 gatggtgaca gccagtttat ccgcagcaat ctgcaggatg ccaaatggtt gatcaagagt
    55141 ctggaaagcc gtaacgatac gctactgcgc gtgagtcgct gtatcgttga acagcagcaa
    55201 gccttctttg agcaaggtga agaatatatg aaaccgatgg tactggccga tatcgcccag
    55261 gctgtcgaaa tgcatgaatc gacgatatct cgcgtgacca cgcaaaaata cctgcatagt
    55321 ccacgaggca tttttgaact gaagtatttc ttttccagtc acgtcaatac cgagggcggc
    55381 ggcgaagctt cctccacggc gattcgtgca ctggtgaaga aattaatcgc ggcggaaaac
    55441 ccagcgaaac cgttgagcga cagcaagtta acctctttgc tgtcggaaca aggtatcatg
    55501 gtggcacgcc gcactgttgc gaagtaccga gagtctttat ccattccgcc gtcaaaccag
    55561 cgtaaacagc tcgtttgacc caaccgataa ggaagacact atgcagctca acattaccgg
    55621 aaataacgtc gagatcaccg aggcactgcg cgaatttgtt acagctaaat ttgccaaact
    55681 tgagcaatat tttgaccgaa tcaaccaggt ctatgttgtt ctgaaagtgg agaaagtcac
    55741 ccacacctca gatgcaacac tgcatgtaaa cggtggtgaa attcatgcca gcgcggaagg
    55801 tcaggacatg tacgctgcca ttgatggctt aattgataag ctggcacgcc agttgaccaa
    55861 acataaagat aaactgaaac aacactaatt gtccgggcaa ttagcatgtg catggcggcc
    55921 tgttgtgcgg cacaacgggc catttgtacg gttaatgctc cgagcctgtt ccactgtttg
    55981 agtgggcagg ttcttaggtg aaattatgac aaataatgat acaactctac agcttagcag
    56041 tgttcttaac agggaatgta cgcgaagccg cgtccactgt cagagcaaaa aacgcgccct
    56101 ggaaatcatc agcgagctgg cggcgaaaca acttagcctg ccgcctcagg tggtttttga
    56161 agctatcctg acgcgggaaa aaatgggcag taccggtatc ggcaatggta ttgccattcc
    56221 gcatggcaaa cttgaagaag atactctgcg tgccgttggc gttttcgttc agctcgaaac
    56281 gcctatagct ttcgatgcca tcgacaacca accggtggac ttactttttg ccctgctggt
    56341 gccggcagac caaactaaaa cgcacctgca tactctgtcg ctggtggcga aacgtctggc
    56401 ggacaaaacc atctgccgcc gtttacgcgc agcccagagc gatgaagagc tgtatcaaat
    56461 cattacggat accgaaggta ctccggatga agcgtagtta ttcggtaatg tctcttttag
    56521 acgttgtgag gagaaacagt acatggtact gatgatcgtc agcggacgtt caggttcagg
    56581 taaatctgtc gccctgcgtg cgctggaaga tatgggtttt tactgcgtgg ataaccttcc
    56641 cgtagtgttg ttacccgatc tggctcgaac tctggccgat cgcgagattt ctgccgccgt
    56701 cagcattgat gttcgtaata tgccggagtc accagaaata ttcgaacagg cgatgagtaa
    56761 cctgcctgac gctttctcac cgcaactact gttcctggat gccgaccgta ataccttaat
    56821 tcgtcgttac agtgacacgc gccgactgca tccgctttcc agcaaaaacc tgtcgctgga
    56881 aagtgctatc gacaaagaaa gcgatttgct ggagcctctg cgttcgcgag cggatctgat
    56941 tgtcgatacc tcagaaatgt ccgttcacga gctggctgaa atgctgcgta cccgtctgct
    57001 gggtaaacgt gaacgcgaac tgaccatggt ctttgagtct ttcggcttca aacacggtat
    57061 ccctatcgat gcagattacg tctttgacgt gcgcttcttg ccgaacccgc actgggatcc
    57121 gaaactgcgt ccaatgacag gtcttgataa acctgtcgcc gcgttcctcg accgccacac
    57181 agaagtacac aattttatct accagacgcg aagctatctt gagctatggt tacctatgct
    57241 ggaaaccaac aaccgtagct acctgacggt cgccattggt tgtaccggcg ggaagcaccg
    57301 ttcggtgtat attgcagagc aactggcaga ctacttccgc tcgcgcggta aaaacgtcca
    57361 gtcacgccat cgtacgctgg aaaaacgtaa accatgaccg tcaagcaaac tgttgaaatc
    57421 acaaacaagc tgggcatgca tgcccggcct gcaatgaagc tgtttgaatt aatgcagggt
    57481 tttgacgctg aagtgctgtt acgtaatgac gaaggcaccg aggctgaagc caacagcgtt
    57541 attgcgctgc tgatgttgga ttctgccaaa ggacggcaga ttgaagttga agcgaccggt
    57601 ccacaggaag aggaagcact ggccgccgtt atcgccctct ttaattctgg ttttgatgaa
    57661 gattaatctt catcactttg acatacaact cccttcaagc tcccccgaca ataagaaaat
    57721 cacgtactga aatcgttctc aatcaacgtc atttgtacat tttgtgcgct tttcactttc
    57781 agaagaacct taagaaaacc ttaagaggca ttgtttaggt tttgtttaag ttaatcgacc
    57841 atactggaga tcgtcagaaa atatttccag gagatggcat gattcgctta tctgaacaaa
    57901 gtcccctggg caccggaaga catcggaaat gttatgcgca tccggaagat gcccaacgct
    57961 gtatcaagat tgtctaccat cgtggcgatg gtggcgataa agagatccgc cgtgagttaa
    58021 agtactacgc gcatcttggt cgccggttaa aagactggag tggaataccg cgctatcacg
    58081 gtaccgttga gacggattgc ggaaccggtt atgtctacga tgtgattgcc gattttgacg
    58141 gcaagccttc cattacgctg accgaatttg ctgaacaatg ccgttacgaa gaagacattg
    58201 cacaactgcg ccagctactg aaacagctga agcgttattt gcaggataac cgtatcgtga
    58261 cgatgtcgct gaagccgcag aatatcctct gtcaccgcat cagtgagtca gaggttatcc
    58321 cggtcgtctg cgacaacatt ggtgaaagca cattgattcc actggcgacc tggtcgaagt
    58381 ggtgttgtct gcgtaagcaa gagcggttat ggaaacgatt tatcgcccaa ccggcgctgg
    58441 ctattgccct gcagaaagat ctgcaacctc gtgagagcaa aacgctggcc ctcacttcgc
    58501 gcgaagctta atccagctgg tgttgttgca taaacggctc accgcctaac tgatacatct
    58561 gccgtaaaat ccacgcctga cggctacgca cgtagcccga tggcgaggag actttgaaac
    58621 gaagtggatt aggtaataca gctgccagta atgcagcttc cgaccgggta agtttgctcg
    58681 cgggtttgtg gaaataacgt tgtgccgcag cttcgacgcc aaacacaccg tcgccaaatt
    58741 cggcgatatt caggtaaacc gtcaggatac gctttttgct ccagactgtt tctatcccca
    58801 gcgttaatcc agcttccagc ccttttcgta cccagctacg cccatcccat aaaaagagat
    58861 tttggccgtc tgctgagaaa tcgttgaagc accgcgaata cggttttcat tgcgctcgtt
    58921 gtgcgccagg gctttctcaa tggaagcgac atcaaagccc cagtgctcag gaaatttctg
    58981 atcttctgcg gcaatcaccg ccagtcccat ccacggcgag atttgatcca tactgaccca
    59041 gtcagaatgt gccacgtaac gaaaattgcc atgcagccag gcgctgacct gtcgctcgac
    59101 cattaccgct gagaagggaa caggcgcaac gctaaacaac gcgatgcccc cgccccagaa
    59161 aacggcgagg acaaccatta accgcaaaag aaaacggcga acgaaactaa acaccgttaa
    59221 gcggctttta ctcattcagc cagaaccagc acgcgggaaa ccagcttatc aatgccgctc
    59281 gccgcttctg caatgttctg cgccagcata tatgctgggg tggtgacaat cttattgtct
    59341 tcatcaacca cgatatcatc gacaggacac ggcacatgct ccgcgcccat ctcttccagc
    59401 acttctgcgg tatcgatatc agtaccgatg gtcaaacgca gcgggaaatc gaaaattttc
    59461 ggcagcatcg ccggggcaat acacataaaa ccaagcggtt ttccggcctg atgcatcgct
    59521 tgtgccagcg cctttaattc acggtcaacg gtgcattcgc taccaagact ggcaaaattg
    59581 cttaaattct tcgccgcgcc aaaccccccc ggcacaatca acgcatccag ttcagcggca
    59641 tcggcctggg ccagaggacg gatttcaccg cgcgttattc gtgccgcttc aatcagcaca
    59701 ttgcgcgttt ccgtcatcgc ttcgccagtt aaatggttga taacatcaac ctgctgctta
    59761 tccggtgcaa agcagaccgc ctgcgcaccg ctgcgtgaaa tagctaacaa cgtcaacacc
    59821 gcttcatgaa tttcagaacc gtcatagacg ccgcatccgc tcagaattac gccaattttc
    59881 ttcattgtga tcatcctttc gcaattgact gaaacacatt aattttttta ataaaaatgg
    59941 tacgcatcac acatttaact gattcatgta acaaatcatt taagttttgc tatcttaact
    60001 gcgtgcggcc tgaaaaacag tgctgtgccc ttgtaactca tcataataat ttacggcgca
    60061 gccaagattt ccctggtgtt ggcgcagtat tcgcgcaccc cggtctagcc ggggtcattt
    60121 tttagtggct tttgccaccc acgctttcag cacttctacg tcgtgacgcc actcttcttt
    60181 catctcttca atccattcac cgacgttatc ttcccaggcg ggaaggtcag gagactgaat
    60241 ttgctgaccc agctgttgca gatggcgtaa ccccactgac cccgccgcac ctttaatttt
    60301 atgtccttcc tcaacaatgc cttttttatc ctgcgccgtc agattcgact ccagcacgct
    60361 gacatagccc ggcatcatct tctcaaacac cgctaacccg tcggtgatca gcttcggtcc
    60421 tacaagttcg agatactgtt ccagcatggg aatatcgagc aatgcttctg atttactgtt
    60481 ctcttctgtc gtcaccgtac tctcctcatc atcctgggta tcccagaatt tcttgatcat
    60541 cgcggttagc gccggaaccg aaagcggctt actcagcaca tcatccattc cagcattgag
    60601 gtactcttgt ttgtctttca gcacgttagc ggttaaggcc accagcggcg gtaaatcctc
    60661 gcgcggataa cgcttcgtca gttcacgaga gatatccagc ccggtcatat ctggcaactg
    60721 aatatccagc aacaccaggt cgtattcgcc cggtttaaac atctccagcg ccgccttgcc
    60781 ggtcatggcg acatcaacgc tgttacctaa tttttccagc acagaacgcg cgacaatcac
    60841 gttcagttca atgtcttcca ccagcagcac attcagcgcc ggtaaaggca tatcgtcttc
    60901 atcaaacgca tcatcgacct cttctgccac cgacggtgcg tggatcgtca acgtaaaggt
    60961 tgaacctttg ccctgttcac tggtaaccgt aatatcgccg cccatatttt tcgccagacg
    61021 acgagaaacg gccagaccaa taccggtgcc ggtggcaggt ttaccgccat gactgtcttt
    61081 cacctggtaa tacatggcga agattttatc cagctcatcc tgcggaatgc cgataccaga
    61141 atcttccact tcaaaatgca gcatatcgcc ttcatcgtag cgcacgcgca cggtaacctg
    61201 gccttgctgg gtgaatttga cggcgttact gatgaggttc cacaggatct gccgtaaacg
    61261 cgtcccgtcg gtaatgacct gatgcggtaa tggcagcgta ggctccaggt taaagcgcaa
    61321 tcctttttgt tgcgcctgca atgcggagag attttccaga tcggcaagga agctggtgaa
    61381 atcaaccggc tgattatcaa gctggacctt gcgccgttcc atcttatcca tgtcgataat
    61441 atcgttaaag atattcccca gcgtgacggc cgaaacatgg atagttttga gatatttttc
    61501 ctgctcggcg gtgagttcgg tatccagcag aatgcggctc aggccgacga taccgttcag
    61561 cggtgtacgc aattcgtgac tgatggtgga aataaacgtc gttttgtcgc ggctggcgcg
    61621 ttcaagcgca tcctgatacc gcttacgctc ggtaatgtcg cgaccaaagc ccatcaaacc
    61681 gtgacgttta cccacgcggt cgtagtacgg cactttacgg atttcaaagc aggctttgcg
    61741 cccgtccggg taatccagcc actgttcata ggtcagtgac acattatgac ggaacacttt
    61801 ttcatcggtt tcaatgactt ttgcggcggc ttccggtgag taaacatcag caggtttcag
    61861 gtgaaccagt tgtttttcgc tttttccggt cagcagctcc atcgcgcggt tacagccgga
    61921 aaactcttta tcttcgttac gataaaaaac caagtcgggt gaagcatcaa ggaaggaacg
    61981 taagaatgag gattgctgct cgagctgaat ttgtgtctct tcgcgctctt tgatttcaat
    62041 tttcagttgg ccgaaggttt cctgcagttc tgcttccgct ttttcacgaa cggcaatttc
    62101 ctgatttagc tgggcaatat tatcttttaa ctgaacgttg aggctcaaat cgcgctcgcg
    62161 catctcctcc agtttttgca ccagccgtga cagacgttgt cgtgactcct ccagttgctc
    62221 gacgaccacc gatagaaagt agaccgccca cggcgtaatc agcaaaccaa agaagataga
    62281 acgaataaca tcaatgcttt cgacctgacc atgcagcacc atggttaccg ccatttgtac
    62341 cacaatggca agaacgacga gggccagcgc cagcaacatt gagaagcgca ccagacctaa
    62401 cttcatcatc aggtcaacat aatactgcgc cagcagacga atttgcttca ttagggaatt
    62461 ccttcacgac aacctgcccc aataataccc aattctgaca gttacgttat aaattgtgtg
    62521 agaaatgcgg aattcccctc aacgacctac tccgttggag gtagccacgg acgtcccagc
    62581 gccgatccct gtacgccatg ttcattcaga tagcgatcca gctcgaccat tcccgtccat
    62641 cgattttcgc accacagcgg agcaagcagc gtcgggcgac gggcgctggc ggaaatgcgg
    62701 tggtagatca cttccggtgg cgtatggcga atcatttctc cggcagtgag cgtataatcc
    62761 tccagttcaa taccgttcaa acgccccgct tcccaggctt tcgccataat gctgcctttc
    62821 acaatatgca gcggatgcag ctttatgccg tccacgctgg tttcaaccac tcgctccagc
    62881 gtttgcaaac attcagcctg cccttcgcca ggcaggccga caattaagtg ggaacacacc
    62941 ttcagcccgc gctgacgtgc cagctgggtt gtacgctgat aacaggcaaa atcatggccg
    63001 cggttgatgc gatgcagtgt tttgtcgtgg gcggtttgta gccccagctc cagccacact
    63061 tcgtaaccct ggtccttata ttcgcaaagc agatccagca ccgcatccgg cacgcagtcc
    63121 gggcgggtac caacacacaa accgacaata ttggcctggc tcaccgcctg ctgatacata
    63181 gaacgcagca cctgaacttc cgcaaaggtg ctggtatacg cctgaaagta ggccagatag
    63241 cgtttagcgc ggttaactaa attcgcctgg tgcgccagtt gctcggcaat ggaacgatgc
    63301 tgctgcgctt catcggcaaa cgaggcaaca ttacagaatg tgcagccacc acgcccgatg
    63361 gtaccgtcac ggttagggca gctaaaaccg ccgtgcagcg tcagcttatg caccttttgc
    63421 ccataacgac gggtgagatc accaccaaac atattgacta atttctgtaa ctgcataatc
    63481 tgatagaccg cgccttgaaa agaggccaaa gcctgccatt tttagctcaa ttcggcgatg
    63541 acctggatca atcgtcccgg cctgctttta tcaactgcat aatcaatcaa aattaccgaa
    63601 atttcatgca caatcacata aatcactttt gcttatcttg tgtcagattt ttttatctcc
    63661 ttatggattt taggcaaaaa cagtagcatg aaacgtcatt accaattaag gcagtataaa
    63721 atgctggttt tctcgtcagt tcaaggcagg ataagggtta acacgccttt atgacagtca
    63781 ggaattgact gtttctctaa cgactctcct tttagcctta aagataaaat ctattttaat
    63841 ttcagctatt taataaagaa ttttgcgcta aagcacattt ctgtaccaat aagcttgcca
    63901 tttgacctgt atcagctttc ccgataagtt ggaaatccgc tggaagcttt ctggatgagc
    63961 agcctgctca tcatatttat gcagtaattg agatcccctc ttcaccgtat taaccgatgc
    64021 gaaaaggaca acaagggggc gaatgcgagg cgcgcgtatg acacgcaaac cccgtcgcca
    64081 cgctctttct gtgcccgtgc gcagcggttc ggaagtgggg ttcccgcaga gcctggggga
    64141 ggttcacgat atgttgtacg ataaatccct tgagagggat aactgtggtt tcggcctgat
    64201 cgcccacata gaaggcgaac ctagccacaa ggtagtgcgt actgcaatac acgcactggc
    64261 ccgcatgcag caccgtggcg cgattctcgc cgatggtaaa accggcgacg gttgcggctt
    64321 gctgttacaa aaaccggatc gcttttttcg catcgttgcg caggagcgcg gctggcgttt
    64381 agcaaaaaac tacgctgtcg ggatgctctt cctgaataaa gatcctgaac tcgccgctgc
    64441 cgcacgccgc atcgttgaag aagaactgca acgcgaaacc ttgtcgattg tgggctggcg
    64501 tgatgtcccc actaacgaag gcgtgctggg tgaaatcgcc ctctcctctc tgccacgcat
    64561 tgagcaaatt tttgtgaacg ccccggcagg ctggcgtcca cgcgatatgg agcgccgtct
    64621 gtttatcgcc cgccgccgca ttgaaaagcg tctcgaagcc gacaaagact tctacgtctg
    64681 tagcctgtcg aatctggtga acatctataa aggtctgtgt atgccggcgg atctgccgcg
    64741 cttttatctg gatcttgcgg acctgcgtct ggaatcggcc atttgcctgt tccaccagcg
    64801 cttctccact aacaccgtac cgcgctggcc gctggcgcaa ccgttccgct atctggcgca
    64861 taacggtgaa atcaacacca tcaccggtaa ccgccaatgg gcgcgtgcgc gtacctataa
    64921 attccagacg ccgctgatcc ctgacctgca cgacgccgcg ccattcgtca acgaaaccgg
    64981 ttctgactcc agttccatgg ataacatgct ggaactgctg ctggcaggcg ggatggatat
    65041 catccgcgcc atgcgcctgt tagtgccacc cgcctggcag aacaacccgg atatggaccc
    65101 ggaactgcgt gccttctttg actttaactc catgcatatg gagccgtggg atggtccggc
    65161 gggcatcgtg atgtccgatg gtcgcttcgc tgcctgtaac ctcgaccgta acggtctgcg
    65221 tccggcacgc tacgtcatta ccaaagacaa gctcatcacc tgcgcctctg aagtcggtat
    65281 ctgggattac cagcctgacg aagtggtcga aaaaggccgc gtcgggccag gcgaactgat
    65341 ggtgatcgac actcgcagtg ggcgtattct gcactcggca gaaaccgatg acgatctgaa
    65401 aagccgccac ccttataaag agtggatgga gaaaaacgtc cgccgactgg taccgtttga
    65461 agatctgccc gatgaagaag tgggtagccg cgaactggac gacgacacgc ttgccagcta
    65521 ccagaaacag tttaactaca gcgcggaaga gctggactcc gtaattcgcg tactgggcga
    65581 aaacggtcag gaagcggtcg gttcgatggg cgatgatacc ccattcgccg tgctctccag
    65641 tcagccgcgc attatttacg actacttccg ccagcagttt gcccaggtga ctaacccgcc
    65701 aatcgacccg ctgcgtgaag cgcatgttat gtcgctcgcc accagtatcg gtcgtgaaat
    65761 gaacgtcttt tgcgaagcag agggccaggc gcaccgttta agctttaaat cgccgattct
    65821 gctctactcc gatttcaaac agctcacgac gatgaaagag gagcactacc gcgcagatac
    65881 gctggatatc acctttgacg tcactaaaac cacgctcgaa gcgacagtca aagagctatg
    65941 cgacaaagcc gaaaaaatgg tacgtagcgg caccgtgttg ctggtgctct ccgaccggaa
    66001 tatcgctaaa gatcgcctgc cggttccggc tccgatggcg gttggcgcga tccagacccg
    66061 tctggtcgat caaagcctgc gttgcgatgc caacatcatc gtcgaaaccg ccagcgcccg
    66121 cgatccgcac cacttcgccg tgttgctggg tttcggcgcg acggctattt atccgtacct
    66181 tgcctatgaa acgctgggcc gcctggtaga cacccacgcg attgccaaag attatcgtac
    66241 cgtgatgctc aactaccgta acggcatcaa caaaggcttg tacaaaatca tgtccaaaat
    66301 gggcatctcc accatcgcct cttaccgctg ctcgaaactg tttgaagcgg tcggtctaca
    66361 cgatgatgta gtgggcctgt gcttccaggg cgcggtcagc cgcattggtg gggcaagctt
    66421 tgaagacttc cagcaggatc tgctgaatct gtcgaaacgt gcctggctgg cgcgcaagcc
    66481 gattagccag ggcggcctgc tgaaatacgt ccacggcggc gaataccacg cctacaaccc
    66541 ggacgtggtg cgcacgctgc aacaggcggt acaaagcggc gagtacagcg actatcagga
    66601 atacgcgaag ctggttaatg agcgtccggc aaccacgctg cgcgatctgc tggcaattac
    66661 gccgggtgaa aacgcggtca acattgctga tgttgaaccg gcaagcgaac tgtttaaacg
    66721 ctttgatacc gccgcgatgt ctatcggcgc gttaagcccg gaagcccacg aggcgctggc
    66781 ggaagcgatg aatagcatcg gcggtaactc gaactccggt gaaggcggcg aagacccggc
    66841 gcgctatggc actaacaaag tgtcgcgcat caagcaggtg gcttctggtc gcttcggcgt
    66901 tactccggcg tatctggtca atgccgacgt cattcagatt aaagtcgccc agggcgcgaa
    66961 gccgggcgaa ggtggtcagt tgccgggcga caaagtcact ccttacatcg ccaaactgcg
    67021 ctattcggtg cccggagtga cgctgatctc cccgccgccg caccacgata tctactctat
    67081 cgaagactta gcgcagctta ttttcgacct caagcaggtt aacccgaaag cgatgatctc
    67141 cgttaagctg gtgtccgaac caggcgttgg caccatcgcg actggcgtgg cgaaagctta
    67201 tgcggacttg atcaccatcg cgggctatga cggcggcacc ggcgcaagcc cgctgtcgtc
    67261 agtgaaatat gcaggctgcc cgtgggaact cggtctggtg gaaacccaac aggcgctggt
    67321 tgccaacggc ttgcgtcata aaattcgttt gcaggttgat ggcggcctga aaacgggtgt
    67381 cgatatcatc aaagcggcga ttctcggcgc agaaagcttc ggcttcggca ctggcccaat
    67441 ggtggcgctc ggctgtaaat atctgcgtat ttgccacctg aacaactgcg ccacgggtgt
    67501 agcaactcag gatgacaaac tgcgtaagaa tcactatcac ggcctgccgt tcaaggtgac
    67561 taattacttt gagtttatcg cccgtgaaac ccgcgagctg atggcacagc tgggagtaac
    67621 gcgtctggtg gatctgattg gtcgcaccga cctgctgaaa gagctggacg gtttcaccgc
    67681 caaacagcag aaactggcgc tgtcgaagct gctggagact gccgagccac atgcaggtaa
    67741 gtcactctac tgcactgaaa acaacccgcc gtttgataac ggcctactga acgcgcagtt
    67801 gctgcaacag gcgaaaccgt ttgtcgatga acgccagagc aaaaccttct ggttcgatat
    67861 ccgcaacacc gaccgttctg tcggcgcgtc gctttcaggc tatatcgccc agacgcacgg
    67921 cgatcagggg ctggcagccg atcctatcaa agcgtacttc aacggcaccg caggccagag
    67981 tttcggtgtg tggaacgcgg gcggcgtgga actgtacctg accggggatg ccaacgacta
    68041 tgtcggtaaa ggcatggcgg gcggcttaat cgccattcgt cctccggttg gttccgcctt
    68101 ccgcagccat gaagcaagca ttatcggcaa cacctgcctg tatggcgcga ccggtggtcg
    68161 tctgtatgcc gcaggccgcg cgggtgaacg tttcggcgtg cggaactccg gtgctatcac
    68221 cgtggtagaa ggcattggcg acaacggctg tgaatatatg acgggcggta ttgtctgtat
    68281 tctgggtaaa accggcgtta acttcggcgc gggcatgacc ggcggcttcg cttacgttct
    68341 cgatgaaagc ggtgatttcc gcaaacgcgt taacccggaa ctggtcgaag tcttaagcgt
    68401 cgacgatctg gcgatccatg aagagcatct gcgcggtctt atcaccgagc atgttcagca
    68461 taccggttct cagcgcggtg aagagattct ggcgaactgg tcaaccttcg ccacgaaatt
    68521 cgcgctggtt aaaccgaagt ccagtgatgt aaaagcactg ctgggtcacc gtagtcgtag
    68581 cgcagcagag ttgcgcgtgc aggcgcagta aggggtagca acaatgagtc agaatgttta
    68641 tcaatttatc gacctgcagc gcgttgatcc gccaaagaaa ccgctgaaga tccgtaaaat
    68701 tgagtttgtt gaaatttacg agccgttttc cgaaggccag gccaaagcgc aggctgaccg
    68761 ctgtctgtcg tgcggcaacc catactgcga gtggaaatgc ccggtacaca actacatccc
    68821 aaactggctg aagctcgcca acgaggggcg tatttttgaa gcggcagaac tgtcgcatca
    68881 gaccaacaca ctgccggaag tatgcggacg tgtctgcccg caagaccgtc tgtgcgaagg
    68941 ttcctgcacc ctgaacgatg agttcggtgc ggtgaccatc ggcaacattg agcgttatat
    69001 caatgataaa gcgttcgaaa tgggctggcg tccggatatg tccggcgtga agcaaaccgg
    69061 taaaaaagtg gcgattatcg gtgcaggccc ggcaggtctg gcgtgtgcgg atgtcctgac
    69121 gcgtaacggc gtaaaagccg ttgtcttcga ccgtcatcca gaaattggcg ggctgctgac
    69181 cttcggtatt ccggccttca agctggaaaa agaggtaatg acgcgtcgcc gtgaaatctt
    69241 caccggcatg ggtattgaat tcaaactcaa taccgaagtg ggccgcgacg tacagctgga
    69301 cgatctgctg agtgattacg atgccgtgtt ccttggcgtt gggacttatc agtcaatgcg
    69361 cggcgggctg gaaaacgaag acgccgatgg cgtgtacgca gcgctgccgt tccttatcgc
    69421 caacaccaaa cagttaatgg gctttggtga aacccgcgac gaaccgttcg tcagcatgga
    69481 aggcaaacgc gtggtagtac ttggcggtgg cgacactgcg atggactgcg tgcgcacgtc
    69541 tgtacgccag ggggcaaagc acgttacctg tgcctatcgt cgtgatgaag agaacatgcc
    69601 gggttcccgc cgcgaagtga aaaacgcgcg ggaagaaggc gtagagttca aattcaacgt
    69661 ccagccactg gggattgaag tgaacggtaa cggcaaagtc agcggcgtaa aaatggtacg
    69721 taccgaaatg ggcgaaccgg atgccaaagg ccgtcgccgc gcggagatcg ttgcaggttc
    69781 cgaacatatc gttccggcag atgcggtgat catggcgttt ggtttccgtc cacacaacat
    69841 ggaatggctg gcaaaacaca gcgtcgagct ggattcacaa ggccgcatca tcgctccgga
    69901 aggcaacgac aacgctttcc agaccagcaa cccgaaaatc tttgctggcg gcgatatcgt
    69961 ccgtggttcc gatctggtcg ttaccgccat cgccgaaggt cgcaaagcgg cagacggcat
    70021 tatgaactgg ctggaagttt aagctctgtc ccagcgccgc ctcctcagaa aagagtcggc
    70081 gctgaaattc tcctttcttt ctggaaatcg cctcgcataa tctgaagaaa accaatgtaa
    70141 tacgctcatc tggattgtta ccggtagatg cccggcgtat agctgattcc attattcaac
    70201 gcagcgccat ataaagggac agataatgga atcgctctca gagagaacct caacaggcta
    70261 ccagcaaatc cacaacggca ttattcatct ggtcgatagc gcccgaacgg aaacggtacg
    70321 tagcgttaac gcgctgatga ccgcgacgta ctgggaaatt ggccgacgga ttgtcgaatt
    70381 tgaacaaggt ggtgaggcca gagcagctta tggtgcacag ttgattgatc ggctatcgaa
    70441 ggatttaagt cgacggtata aacgtggggt ctctgcaaaa aacttacgcc aaatgaggct
    70501 tttttacctc ttttttcaac atgttgaaat tcgccagaca gtgtctggcg aattaacacc
    70561 attgggaatt ccgcagacac catctgcaga attcccatcg gcaaagattt ggcagacgct
    70621 gtctgccaaa ttgagaggct gtttgatatt tccacagctt ttgtattgca tctcctattt
    70681 tcggtttgta tattcatgca aataaacgaa gg
//
LOCUS       DAEBXN010000004        66992 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.004, whole genome shotgun sequence.
ACCESSION   DAEBXN010000004 DAEBXN010000000
VERSION     DAEBXN010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 66992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66992
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.004"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            1..583
                     /locus_tag="KJL99_000244"
                     /pseudo
     CDS             1..583
                     /locus_tag="KJL99_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
     gene            686..1009
                     /locus_tag="KJL99_000245"
     CDS             686..1009
                     /locus_tag="KJL99_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1877 family protein"
                     /protein_id="HBD4435127.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLESLD
                     RSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNHAL
                     TVIVG"
     gene            complement(1076..2533)
                     /gene="nuoN"
                     /locus_tag="KJL99_000246"
     CDS             complement(1076..2533)
                     /gene="nuoN"
                     /locus_tag="KJL99_000246"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HBD4435128.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2540..4069)
                     /gene="nuoM"
                     /locus_tag="KJL99_000247"
     CDS             complement(2540..4069)
                     /gene="nuoM"
                     /locus_tag="KJL99_000247"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HBD4435129.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMTFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4233..6074)
                     /gene="nuoL"
                     /locus_tag="KJL99_000248"
     CDS             complement(4233..6074)
                     /gene="nuoL"
                     /locus_tag="KJL99_000248"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HBD4435130.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFRDMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRSLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6071..6373)
                     /gene="nuoK"
                     /locus_tag="KJL99_000249"
     CDS             complement(6071..6373)
                     /gene="nuoK"
                     /locus_tag="KJL99_000249"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HBD4435131.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6370..6924)
                     /gene="nuoJ"
                     /locus_tag="KJL99_000250"
     CDS             complement(6370..6924)
                     /gene="nuoJ"
                     /locus_tag="KJL99_000250"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HBD4435132.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(6936..7478)
                     /gene="nuoI"
                     /locus_tag="KJL99_000251"
     CDS             complement(6936..7478)
                     /gene="nuoI"
                     /locus_tag="KJL99_000251"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HBD4435133.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7493..8470)
                     /gene="nuoH"
                     /locus_tag="KJL99_000252"
     CDS             complement(7493..8470)
                     /gene="nuoH"
                     /locus_tag="KJL99_000252"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HBD4435134.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDRVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8467..11193)
                     /gene="nuoG"
                     /locus_tag="KJL99_000253"
     CDS             complement(8467..11193)
                     /gene="nuoG"
                     /locus_tag="KJL99_000253"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HBD4435135.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRYASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSSASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11246..12583)
                     /gene="nuoF"
                     /locus_tag="KJL99_000254"
     CDS             complement(11246..12583)
                     /gene="nuoF"
                     /locus_tag="KJL99_000254"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HBD4435136.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12580..13080)
                     /gene="nuoE"
                     /locus_tag="KJL99_000255"
     CDS             complement(12580..13080)
                     /gene="nuoE"
                     /locus_tag="KJL99_000255"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HBD4435137.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13083..14885)
                     /gene="nuoC"
                     /locus_tag="KJL99_000256"
     CDS             complement(13083..14885)
                     /gene="nuoC"
                     /locus_tag="KJL99_000256"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HBD4435138.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(14979..15641)
                     /gene="nuoB"
                     /locus_tag="KJL99_000257"
     CDS             complement(14979..15641)
                     /gene="nuoB"
                     /locus_tag="KJL99_000257"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HBD4435139.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15657..16100)
                     /gene="nuoA"
                     /locus_tag="KJL99_000258"
     CDS             complement(15657..16100)
                     /gene="nuoA"
                     /locus_tag="KJL99_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HBD4435140.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(16731..17669)
                     /gene="lrhA"
                     /locus_tag="KJL99_000259"
     CDS             complement(16731..17669)
                     /gene="lrhA"
                     /locus_tag="KJL99_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HBD4435141.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKVDIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            18588..19805
                     /gene="alaA"
                     /locus_tag="KJL99_000260"
     CDS             18588..19805
                     /gene="alaA"
                     /locus_tag="KJL99_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HBD4435142.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            19889..20488
                     /gene="yfbR"
                     /locus_tag="KJL99_000261"
     CDS             19889..20488
                     /gene="yfbR"
                     /locus_tag="KJL99_000261"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HBD4435143.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNAQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLMKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(20547..22379)
                     /locus_tag="KJL99_000262"
     CDS             complement(20547..22379)
                     /locus_tag="KJL99_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HBD4435144.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTSVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTNEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILTVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(22466..23116)
                     /gene="hxpA"
                     /locus_tag="KJL99_000263"
     CDS             complement(22466..23116)
                     /gene="hxpA"
                     /locus_tag="KJL99_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HBD4435145.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEADLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(23127..23621)
                     /locus_tag="KJL99_000264"
     CDS             complement(23127..23621)
                     /locus_tag="KJL99_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HBD4435146.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMDMYHALYVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(23704..24159)
                     /locus_tag="KJL99_000265"
     CDS             complement(23704..24159)
                     /locus_tag="KJL99_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HBD4435147.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGLLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            24497..25699
                     /gene="ackA"
                     /locus_tag="KJL99_000266"
     CDS             24497..25699
                     /gene="ackA"
                     /locus_tag="KJL99_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HBD4435148.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVAFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            25774..27918
                     /gene="pta"
                     /locus_tag="KJL99_000267"
     CDS             25774..27918
                     /gene="pta"
                     /locus_tag="KJL99_000267"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HBD4435149.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            28108..29628
                     /gene="yfcC"
                     /locus_tag="KJL99_000268"
     CDS             28108..29628
                     /gene="yfcC"
                     /locus_tag="KJL99_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBD4435150.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGNASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(29661..30203)
                     /gene="yfcD"
                     /locus_tag="KJL99_000269"
     CDS             complement(29661..30203)
                     /gene="yfcD"
                     /locus_tag="KJL99_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HBD4435151.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSGVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(30261..30812)
                     /gene="yfcE"
                     /locus_tag="KJL99_000270"
     CDS             complement(30261..30812)
                     /gene="yfcE"
                     /locus_tag="KJL99_000270"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HBD4435152.1"
                     /translation="MKLMFASDIHGSLPATERVVELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMMLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPAGYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(30868..31512)
                     /gene="yfcF"
                     /locus_tag="KJL99_000271"
     CDS             complement(30868..31512)
                     /gene="yfcF"
                     /locus_tag="KJL99_000271"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HBD4435153.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            31648..32295
                     /gene="yfcG"
                     /locus_tag="KJL99_000272"
     CDS             31648..32295
                     /gene="yfcG"
                     /locus_tag="KJL99_000272"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HBD4435154.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESAAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRLRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            32352..32714
                     /gene="folX"
                     /locus_tag="KJL99_000273"
     CDS             32352..32714
                     /gene="folX"
                     /locus_tag="KJL99_000273"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HBD4435155.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            32735..33628
                     /locus_tag="KJL99_000274"
     CDS             32735..33628
                     /locus_tag="KJL99_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HBD4435156.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHKQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPTTAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(33676..34566)
                     /gene="rpnB"
                     /locus_tag="KJL99_000275"
     CDS             complement(33676..34566)
                     /gene="rpnB"
                     /locus_tag="KJL99_000275"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HBD4435157.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARNFINIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(34763..35536)
                     /gene="hisP"
                     /locus_tag="KJL99_000276"
     CDS             complement(34763..35536)
                     /gene="hisP"
                     /locus_tag="KJL99_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HBD4435158.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(35544..36260)
                     /locus_tag="KJL99_000277"
     CDS             complement(35544..36260)
                     /locus_tag="KJL99_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBD4435159.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(36257..36943)
                     /gene="hisQ"
                     /locus_tag="KJL99_000278"
     CDS             complement(36257..36943)
                     /gene="hisQ"
                     /locus_tag="KJL99_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HBD4435160.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNMVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(37033..37815)
                     /gene="hisJ"
                     /locus_tag="KJL99_000279"
     CDS             complement(37033..37815)
                     /gene="hisJ"
                     /locus_tag="KJL99_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HBD4435161.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIELAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(38036..38818)
                     /gene="argT"
                     /locus_tag="KJL99_000280"
     CDS             complement(38036..38818)
                     /gene="argT"
                     /locus_tag="KJL99_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HBD4435162.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYALFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDYAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(39084..39653)
                     /gene="ubiX"
                     /locus_tag="KJL99_000281"
     CDS             complement(39084..39653)
                     /gene="ubiX"
                     /locus_tag="KJL99_000281"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HBD4435163.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTLDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(39748..41265)
                     /gene="purF"
                     /locus_tag="KJL99_000282"
     CDS             complement(39748..41265)
                     /gene="purF"
                     /locus_tag="KJL99_000282"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HBD4435164.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(41302..41790)
                     /gene="cvpA"
                     /locus_tag="KJL99_000283"
     CDS             complement(41302..41790)
                     /gene="cvpA"
                     /locus_tag="KJL99_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HBD4435165.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIHGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(42049..42711)
                     /gene="dedD"
                     /locus_tag="KJL99_000284"
     CDS             complement(42049..42711)
                     /gene="dedD"
                     /locus_tag="KJL99_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HBD4435166.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEVPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(42701..43969)
                     /gene="folC"
                     /locus_tag="KJL99_000285"
     CDS             complement(42701..43969)
                     /gene="folC"
                     /locus_tag="KJL99_000285"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HBD4435167.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGLEKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(44039..44953)
                     /gene="accD"
                     /locus_tag="KJL99_000286"
     CDS             complement(44039..44953)
                     /gene="accD"
                     /locus_tag="KJL99_000286"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HBD4435168.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDELKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQAQEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPRFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(45109..45768)
                     /locus_tag="KJL99_000287"
     CDS             complement(45109..45768)
                     /locus_tag="KJL99_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBD4435169.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VMPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(45851..46663)
                     /gene="truA"
                     /locus_tag="KJL99_000288"
     CDS             complement(45851..46663)
                     /gene="truA"
                     /locus_tag="KJL99_000288"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBD4435170.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(46663..47676)
                     /locus_tag="KJL99_000289"
     CDS             complement(46663..47676)
                     /locus_tag="KJL99_000289"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBD4435171.1"
                     /translation="MSEGWNIAVLGATGAMGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(47742..48878)
                     /gene="pdxB"
                     /locus_tag="KJL99_000290"
     CDS             complement(47742..48878)
                     /gene="pdxB"
                     /locus_tag="KJL99_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HBD4435172.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            48977..49972
                     /gene="flk"
                     /locus_tag="KJL99_000291"
     CDS             48977..49972
                     /gene="flk"
                     /locus_tag="KJL99_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HBD4435173.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNHRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(49969..51147)
                     /locus_tag="KJL99_000292"
     CDS             complement(49969..51147)
                     /locus_tag="KJL99_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD4435174.1"
                     /translation="MTAVSQTETRSSASFSLFRISFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(51402..52643)
                     /gene="fabB"
                     /locus_tag="KJL99_000293"
     CDS             complement(51402..52643)
                     /gene="fabB"
                     /locus_tag="KJL99_000293"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HBD4435175.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNIKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAYIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKRLIRSRPE"
     gene            52802..54808
                     /gene="mnmC"
                     /locus_tag="KJL99_000294"
     CDS             52802..54808
                     /gene="mnmC"
                     /locus_tag="KJL99_000294"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HBD4435176.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVIAESGFGTGLNFLTLWKAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDESRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(54929..55207)
                     /locus_tag="KJL99_000295"
     CDS             complement(54929..55207)
                     /locus_tag="KJL99_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HBD4435177.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQL"
     gene            complement(55241..55789)
                     /gene="epmC"
                     /locus_tag="KJL99_000296"
     CDS             complement(55241..55789)
                     /gene="epmC"
                     /locus_tag="KJL99_000296"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HBD4435178.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(55789..56598)
                     /locus_tag="KJL99_000297"
     CDS             complement(55789..56598)
                     /locus_tag="KJL99_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBD4435179.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVMLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(56598..57422)
                     /gene="mepA"
                     /locus_tag="KJL99_000298"
     CDS             complement(56598..57422)
                     /gene="mepA"
                     /locus_tag="KJL99_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HBD4435180.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(57426..58511)
                     /gene="aroC"
                     /locus_tag="KJL99_000299"
     CDS             complement(57426..58511)
                     /gene="aroC"
                     /locus_tag="KJL99_000299"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HBD4435181.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     FGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(58546..59478)
                     /gene="prmB"
                     /locus_tag="KJL99_000300"
     CDS             complement(58546..59478)
                     /gene="prmB"
                     /locus_tag="KJL99_000300"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBD4435182.1"
                     /translation="MDKIFVDEAVNELQTIQDILRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            59644..60195
                     /gene="smrB"
                     /locus_tag="KJL99_000301"
     CDS             59644..60195
                     /gene="smrB"
                     /locus_tag="KJL99_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HBD4435183.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(60299..61189)
                     /locus_tag="KJL99_000302"
     CDS             complement(60299..61189)
                     /locus_tag="KJL99_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HBD4435184.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNELKFVVLL"
     gene            complement(61176..61700)
                     /locus_tag="KJL99_000303"
     CDS             complement(61176..61700)
                     /locus_tag="KJL99_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBD4435185.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(61697..62167)
                     /locus_tag="KJL99_000304"
     CDS             complement(61697..62167)
                     /locus_tag="KJL99_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBD4435186.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVILPEADFEAWATLQVDYQ"
     gene            complement(62164..62712)
                     /locus_tag="KJL99_000305"
     CDS             complement(62164..62712)
                     /locus_tag="KJL99_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBD4435187.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINRVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(62717..63439)
                     /locus_tag="KJL99_000306"
                     /pseudo
     CDS             complement(62717..63439)
                     /locus_tag="KJL99_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(63459..64181)
                     /locus_tag="KJL99_000307"
     CDS             complement(63459..64181)
                     /locus_tag="KJL99_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001433219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HBD4435188.1"
                     /translation="MAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMGGT
                     RLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEATQS
                     VVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDDGN
                     VYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDIKP
                     VIQEQTQQVTPTNPPVSVSANQ"
     gene            complement(64241..65395)
                     /gene="yfcU"
                     /locus_tag="KJL99_000308"
                     /pseudo
     CDS             complement(64241..65395)
                     /gene="yfcU"
                     /locus_tag="KJL99_000308"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
     gene            complement(65344..66099)
                     /locus_tag="KJL99_000309"
     CDS             complement(65344..66099)
                     /locus_tag="KJL99_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001435789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HBD4435189.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDMKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDRD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKNGSGVATMPGGRYHH"
     gene            complement(66181..66744)
                     /locus_tag="KJL99_000310"
     CDS             complement(66181..66744)
                     /locus_tag="KJL99_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBD4435190.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     NAWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        16689 a        18149 c        16690 g        15464 t
ORIGIN      
        1 taccaaccct gggtcactgc attatcgcca acgccccgac acgtaagtcc tgagcaatta
       61 gaaaaaacag ttcgttatct tacacaaact gtgcatccac gtaatgccgg caatattgat
      121 aatctgaata ggtccgcaga gtatataaaa gagttctttg tcagtagcgg tgccagagtt
      181 accgcgcagg gcgtccccga ttggctatta cgactctgcg cctggaagcc agaattaccc
      241 ttacccggca atgtcctggc tttatcccga tcggggagac tttattgctg tggtcggcag
      301 aatgcaggat atcaacgccg ttcgtcaggt aaaagcggca ttgttgtcat cccgggattt
      361 atctgtttat tctatgaatg ccccagggtt tattcccggt attgattttt ctgatcacct
      421 caattattgg caacacgata ttcccgccgt aatgattact gacaccgctt tttatcgtaa
      481 taaacaatac cacttgcccg gtgataccgc agacagattg aattatcaga aaatggctca
      541 ggtggtagat ggtgttataa ccttgttata caacagtaaa taataaaaat ttctcgaggc
      601 gggtccagaa acagcgacat gtacatttta ttttccctcg tctgttttaa gatgcgaaac
      661 ataaaattca atggaggaaa aataaatggg aatgattggc tattttgcag aaattgactc
      721 agaaaagatt aaccaactgc ttgagtctac tgaaaaacct ttgatggata atatacatga
      781 cacgctttca ggattacgcc gactggaatc actggatcga agtgagctgc gtaaacagtt
      841 ttccattaag cgactcaatg aaatggagat ttatccggga gtgacctttt cagaagagtt
      901 agaggggcaa ctctttgcct cgatcatgct tgatatggaa aagctgatta gcgcgtatcg
      961 acgtatgctg cgacaaggga atcatgcgct gacggttatt gttgggtgat ggtgtcagag
     1021 cgccaaaaaa aaccgccgaa catgtcggcg gctttctgac ttacaaacta acagtttaca
     1081 tcagcggcat tgctaaccgc acaatactaa tcagcggttg tggccataca cccagcacca
     1141 gtaccaacag tgcagaaatc agcaccacga taccgcccgc gctgtactgc cagtttgacg
     1201 gtgcatcgcg acccggttgt tccggtgcgt gcaggtacag gctcaccgcc acgcgcaggt
     1261 agtagtagag gccgattgcc gaaccgacaa ccacggcacc caccagccac cacaagtgtg
     1321 cctggacacc gaccgccagc acgtagaact taccgataaa gcccagcgtc atcgggatac
     1381 cggccagaga cagcatcatc accgtcatca ctgccgcgag gatcggacga tgccagaaca
     1441 gaccgcggta agagaacagg gaatcagcat ccgggccacg atacgggctg gacatcaggc
     1501 tgaccacgcc gaacgcgccg aggctgctga acagataacc ggccaggtaa acccctaccg
     1561 cttccatcga catctcgccg gtttgcagcg caatcagcgc caccagcaga tagccgaggt
     1621 gagagataga tgagtaaccg agcagacgtt tgatgttggt ctggctcaac gccatcaggt
     1681 taccgaagat gatggaggca aaggcgataa tcgccagtac tacgcgaatc gcttcgctgt
     1741 cacccaccgg tgcgtagagg aacagacgca tcaccacacc gaagatagcg attttgctcg
     1801 ccgtcgccag gaaagtggaa accggcgcag gcgcgccctg gtatacgtct ggcgtccaca
     1861 ggtggaacgg caccagagag agtttgaagc cgaggccaac aatcatcatg ccgaaacctg
     1921 ccagcaacag cggctcgttg agcataccgt cgccgaggtt tttacccagc gcgacaaacg
     1981 acaggtcgcc agactgcgca tacaccagcg ccataccgaa cagcaggaaa gaagacgctg
     2041 cggcagaaag gatggtgtat ttgatactgg cttccagcga acgtttctgg cggaaagcgt
     2101 aaccgaccag gccaaacagc ggcaaagaga tcagttcaat accgaggaac agagacgcca
     2161 gatggtttgc attcgccagc aggatcccgc ccagcgcggc aattaacacc aacaggtaga
     2221 actcatcctt gttgtcgtta tagccttcaa gccacgggta ggcgaaagta caggtggcga
     2281 ggctcgccaa caataccagc ccggtgtaaa gcatggcgaa accatcaacg cgcatcagcg
     2341 gcgtaacgtc catagcgccc gcctggccaa caaaccagag cgaaaccagc gccgcgttaa
     2401 gcccaataac cgagagcgta gcgttgagga aatgattgcg tcgccacgca atggagagca
     2461 tcacaaccac caccgtcaag ccgacgatca gcaacggtag cagtgcgatc aggttttgtg
     2521 gagttattgt catggcgatt tacggccttg tagtagtaac ggaattaaca aaccactgct
     2581 ggatattgcc aatcgcggag tgcgaggtat ccagaatcgg ctgcggatag aagcccagca
     2641 gtaccagcag caccaccagc aacaggatca taaacagctc acgcagcgac atccctggca
     2701 gttcctggct ggcaatctgg cttttcgctt taccgaagta agcgcgatgc aacatcgcca
     2761 gcgaataaac agatgcaaag accagcccaa aggtagagat aacggtaatc accgggacaa
     2821 cctggaagct gccgaacaga atcataaatt cgccgacgaa gttaccggtg ccaggcatcc
     2881 caagcgttgc caccgcaaag aacagcgaca gtgctggcag ccatttcatc ttgctccaca
     2941 gaccgcccat catgcgcatg tcgcgggtat ggatacgttc ataaagctga ccgcagagaa
     3001 taaacagacc cgccgccgac aagccgtgcg caatcatctg gattaccgcg ccctggtagg
     3061 ccaactggct gccggtgtag attgcaatca gcacgaagcc catgtgggaa accgaggtgt
     3121 aggcgatcag acgtttgata tcggtctgag cgaaggtcat ccacgcaccg tagaagatgc
     3181 cgataacacc cagccacata gcgattggcg cgaactctgc cgacgcgttc gggaacagcg
     3241 gcagggagaa acgcaacaaa ccgtaagcgg cggttttcag caagatcccc gcgaggtcca
     3301 cggaaccggc ggtcggagcc tgggagtgcg catccggcag ccagccatgc agcggaacca
     3361 ccggcatttt gactgcgaag gcgatgaaga agcccagcat caacagatat tccacaccac
     3421 tggacattgg cgtattcagc agctcttcat agttgaaggt ccagacgccg gtcgcattgt
     3481 agtgaacaaa aaccagcgcc aggatggcga tcaacatcac cagaccactc gcctgggtgt
     3541 aaatgaagaa cttggttgcc gccgtgatac gcgttttacc gtcagaggct ttatgccccc
     3601 acagtgcgat caggaagtac atcggcacca gcatcatttc ccagaagaag aagaacagga
     3661 acatgtcgat ggcaaggaac acgccgataa cgccgcccag gatccacatc aggttgaggt
     3721 ggaagaagcc ctgatatttt tcgatctctt tccacgaaca tagtaccgcc agcacaccga
     3781 gcagaccggt cagcacgacc atcagcagcg acagaccgtc aatggcgaga tgaatagaga
     3841 taccaaaacg cgggatccac ggcatgtcga attcagactg ccactgcgga attccggcgg
     3901 attgcgtcag tgaataaccg ccctgcaacc acagttgcag cgacagcgcc agcgtcaatc
     3961 ccatggtgat cagcgcgatc cagcgtggca ccttgacgcc aaagcgttcg gtctgccagc
     4021 acaggaagcc gccaataaag ggaattaata ttagccaggg tagtaacatg gcgatcttta
     4081 ttccttgtaa aagtcccgtc aggaccggat tttcaacgaa ttctcacgac agaaaattct
     4141 cttcggactg ggggtagtgt cggatgcgcc gcgaaggcag cttatccgac ctacgttctt
     4201 atatgcccag gggcaatccc acaatcctta actcaacgca gtaccatcaa cagtgccagc
     4261 accacgaccg caccgatgct catggatgcc acataccagc gcagataacc gttctcgctt
     4321 aacagcagac ctttacctgc aaagcgggaa agaaccgccg ggatgttcat cattgagttc
     4381 agcggatcgc gtttcagcaa ccaggcaata cccaggaacg gcttgacgaa cactttgtcg
     4441 tacagccagt caaagcccca agcgttgtac caccaggtac tcagcaggcg gcccggcgca
     4501 ctgttggcga tggaggtcac cagagaacgt ttacccagcc acagccaggc tgccagcaga
     4561 atgccgacca ccgcgaccac gccagaggta atttccaggg tcaacatgct gccgtgcgcc
     4621 agttccgtcg tttgcggaag cacgccctgc agcggcggta caatcagtgc gccaacgaag
     4681 gtggaaagga tcagcagcac aatcagcggc aggctgtgag ttaccccttt cacggcgtga
     4741 gcgtgaattt gttcttttcc gtggaagacg atgaaaatca tacggaaggt gtagagcgag
     4801 gtcataaacg caccgaccag acctgccacc atcagattga tatgaccatt cgccatcgca
     4861 cccgcgagga tctcatcctt actgaagaag cccgcagtga ccagcggcag tgccgacagt
     4921 gctgcgccgc ccaccaggaa gcagagataa accagcggaa tagatttacg cagaccgccc
     4981 atcttgaaga tgttctgttc gtgatggcag gccagaatga cggaaccgga tgccaggaac
     5041 agcagcgctt taaagaacgc gtgagtcatc aagtggaaaa tcgccgcatc ccatgcctgc
     5101 acgccaagcg cgaggaacat gtagccaatc tggctcatgg tagagtaagc gagaacacgt
     5161 ttgatgtcgg tctgtaccag cgcggcaaaa ccggccagca gcagcgtaac cgccccgaca
     5221 atacccacca gatgcagaac ttccggcgtc atcaggaaca ggccgtgggt acgggcgatc
     5281 aggtagacac ccgcggttac catcgttgcg gcgtggatca gcgcggagac aggcgtcggg
     5341 cccgccatcg cgtcagcaag ccatgtctgc aacggcaact gcgcagattt accgaccgca
     5401 ccgcccagca gcatcagcgt cgcccacatc agcatgttat tgccgtcagc aaagtgcgct
     5461 ggtgccagtt ccaccatatc gcggaagttc agggtgccca gttcgttgta aagaatgaac
     5521 agtgcgaaag cgaggaaaac gtcacccaca cgggtcacga cgaacgcttt cattgctgct
     5581 gcgccattct tcggatcggt gtaatagaaa ccgatcagca gataggagca caggcccacg
     5641 ccttcccagc cgaggtacat cagcagcagg ttatcggcga gaaccagaac taccatgctg
     5701 gcgatgaaca ggttggtgta ggcgaagaag cgagagtagc cctcttcacc gcgcatgtac
     5761 caggaggcgt acatgtgaat aaggaaaccc acaccagtga ccaccgagag catggtcagc
     5821 gacaggccgt ccagcaccag gttaaaaccg atgttaaagt cgcctaccga catccacgtc
     5881 cacagcggct ggctgtatgc ctgctcgccg ttagcgaaga aatcaacgcc gataaaggcg
     5941 gttaccagcg ccgccaggcc cacagagcca acgccgacga tcgccgaaac gttttcagac
     6001 cagcgcccac gggagaacgc cagcaggacg aagccaatca atggcaaaat aatggttaag
     6061 gcaagcatgt tcatccgcgc atctcactta ctgaatcgat gttcaggttc tggcgacgac
     6121 ggtgaagttg cagcagcagc gcaaggccga tactcgcttc tgccgccgcg aggctgatgg
     6181 cgagaatgta catcacctga ccgtcggtct gaccccagta gcttccggcg accacaaagg
     6241 ccagcgcgga ggcgttaatc atgatttcca gaccaatcag cataaacagc agattgcgac
     6301 ggataaccag accggttaag ccaagaacga ataagattgc cgcgaggatc agtccatgtt
     6361 gtaaggggat catgcgtgct cctccgtttt tcttttcgcg ctgtcgtctt tacgattgct
     6421 cagcacttca cccgcacgct cttcacgacc gacgtggaag gccacaacca gacccgcgag
     6481 cagcagcata gaagccagtt ccaccgccag tacgtaaggc ccgaacagcg taatacccac
     6541 tgctttagca ctgattggcg taccgtcgat accctgatcg ttaacaccga ggatggcgta
     6601 aacaatcacc accagcatga tggccgacaa aattgccggg ccaatccaca cctgcggttt
     6661 cagccactgg cgttcctgtt cgatttctga accgcccagg ttgagcatca tcaccacgaa
     6721 cacgaacagc accataatgg cacccgcgta gacgataatt tccagcgcac cggcgaagta
     6781 agcgcctagt gagaagaaca ccccggaaat cgccagcagc gaaataatca ggtacagcag
     6841 tgcgtgtacc ggattggtat gggtgatcac tcgcaaggtc gcaagtatgg ctatcaggcc
     6901 acagatataa aaagcgaact ccattgcccc tctccttacg gtaacaggct cttgacgtcg
     6961 ataggcttgg cttcgttctc tgcttcgccc ttatctttgc cgtcgattgc catacctgcc
     7021 atccggtaga agttatattc cgggtatttg cccggaccgg agatcagcag atcctctttc
     7081 tcgtaaacca gatcctggcg cttgtattcc cccatttcga aatccggggt taactgaatc
     7141 gccgtggtcg gacaggcttc ttcgcacaga ccacagaaaa tgcagcgtga gaagttaatg
     7201 cggaaaaatt ccggatacca gcgaccgtct ttggtttctg ctttttgcag cgagatacag
     7261 ccgaccgggc aggctaccgc acagaggtta caggctacgc aacgctcttc gccgtccggg
     7321 tcgcgggtca gaacgatacg accacgataa cggggcggca gatagaccgg ctcttccggg
     7381 tacattcgcg tttcgcgttt ggcgaacgcg tgcaggccga tcatccagat actacgaacc
     7441 tgggtgccga aacctactaa caattctttt aaggtcatgg tcttattgcc ccttattgcg
     7501 cctgccagag aatgacagcc gccgttacca gcaagttgat cagcgtcagc ggcaggcaga
     7561 ttttccagcc gaaggacatt acccggtcat aacgcggacg cggtaacgac gcacgaatca
     7621 aaatgaacat catcataaag aacgcggttt tcagcgcgaa ccagatgaat ggcggtaaca
     7681 acgggccttg ccagccaccg aagaacagcg tcaccatcaa tgcagagatg gtcacaatcc
     7741 cgatgtactc acccacgaag aacagaccga acttcatacc ggaatattca atgtggtaac
     7801 catccgccag ttcctgctcg gcttccggct ggtcaaacgg gtgacggtga cacaccgcca
     7861 cgcccgcgat ggcaaaggta ataaaaccaa agaattgcgg gatgacgttc cacacatgcg
     7921 cctggctgtt gacgatgtcg gtcatgttga atgaaccggc ctgcgccacc acgcccatca
     7981 gggaaagccc gaggaacact tcgtagctca gggtctgcgc agaagcacgc atcgcaccca
     8041 gcagcgagta tttgttgtta cttgaccagc ccgcaaacag caccgcgtaa accgccagac
     8101 ctgccatcat caggaagaac aaaatcccga tgttcaggtc ggcaaccacc caacccggac
     8161 tgactggcac aattgcaaag gccagcagca gcgaggtaaa ggcaatcatc ggtgccaggg
     8221 taaagatgac gcgatccgag aatttcggga tccagtcttc tttaaagaac attttgatca
     8281 tgtccgcaac cagctggagc gaaccgcccc agccaacacg gttaggtcca taacggttct
     8341 ggaacagacc cagcaggcga cgttcgccaa agctcatgaa tgccccacag gtgacaacca
     8401 ccagcaggat caccaccgct ttgaggatgg tcagcaggat ctcaatcagt tccggtgata
     8461 tccaactcat tgttgtgcct ccttgagatc ctcaagatgc gcgccagcca gcaccggagc
     8521 aatgccggac atacccatcg gcaagcccac ctgccctgcc gtcagtcctt cggcgatttc
     8581 aaccggcagc gtgaccgtgt tgccatcgta actaaaggag acgcgtgtac ctgcgttcac
     8641 acccaacttc gcggcatccg ctgggttgag tttgatgtac ggctgcggca tacggctctg
     8701 gaagaccgga gcacgctgtg acaattcatc gctgccaaac aggtggtagt acggcgcgat
     8761 acgccatttc ccgtcctgcg gctggaagcg tgccggtacg ctggtgaagt aatccagacc
     8821 gttttcgctg gtttcaaaca gacgcacgcc cggatcgcca aagcgcagtt tgccgcccac
     8881 ttcgtcctgg aatttgttcc acgcctgcgg ggagttccag cccggcgccc aggcaaacgg
     8941 cacttgcgaa cggtgcgcgg tcggctggtt gttaccttcc atcgagaagg tgaacatggt
     9001 gtcaatatcc tgcggctgac gcggctcatg aacgctgata ttggcgcgca tggcggtacg
     9061 accgctgtaa cggtgcggtt cacgggccag tttctgccca cgaatacgga atgtcgcatc
     9121 cggcgcagca tctttgatac ccgccagttc tgggattttc gccacaacag cgtcaattac
     9181 atggtcgagc tgcgtccagt ccacttcacg gctcagcaag gtgctgtgca gcgagtgcag
     9241 ccagcgccag ctttccagca tgacagtttt gctgtcgtaa taagccggat cgtaaacctg
     9301 gaagaaacgt tgggcgcgac cttcattatt aattaccgta ccgtcgcttt cagcaaagct
     9361 ggcagaggaa agcaccagat gggcgttttc catgatcgct gtgcgttgat gatcaaccac
     9421 catcaccagc ggcgctttag ccagcgcagc attcacgcgg gtagcagaag cgtaacgatg
     9481 cagatcgttt tccaacacca ccaccgcgtc ggcgcgtccg gtttccagtt cggttaacgc
     9541 ttcttcaagc gaaccgccac ccataatgcc cagccccatg ctgttgacgg aacgggcaat
     9601 catggtgata ccaacgtcag cgccgcgacc tttcagggct ttcgcgacgt tagccgcagc
     9661 ctgaatcacc tctatgctac cggcgttcgt cccggagata atcaacggtt tcttcgcacc
     9721 tgccagtgcc tgcacgatga cgtcgatttt gttttgcagc tcaggttcga taccgtcaac
     9781 cgctggtgcg gagttatcca gcgcatgggc gatggcaaaa cctaaacgcg cctgatcttc
     9841 aaccggtgcg cggtaagtcc acgccgcgat atcatccaga cgagtgtcat caacgttggt
     9901 aacaaacagc ggatgcttcg cacgttgacc gatgttgagg attgccgcaa tctgccagtc
     9961 agccactttc tgtgctgcag ccatttcgcg cgctttacct ttcaccgcct gacgcactgc
    10021 cagtgcgacg cgcgcgccgg tctgggtaac gtcttcgccc agcaccagta ccgcatcgta
    10081 agattcgatt tcgcgcagag ccggagtata aatgccgcct tcacgcagca ctttcagcgc
    10141 cagttgcaga cgttcctgct caccgtgagc gataccggtg tagaagtttt cttcgcccac
    10201 cagttcacgc agcgcaaagt tgctttccac gctggcacgc ggagaaccaa taccgatcac
    10261 tttcttcgac tgacgcagaa tatctgccgc gccctgcatt gcctgttcgg cgttgagggt
    10321 aatgaaatca tcgccacgac gctgtactgg ctgacgcgga cgatccttca ggttgacgta
    10381 accgtaaccg aaacgaccac ggtcgcagag gaagtagtgg tttaccgtac cgttgtaacg
    10441 gttttcgata cgacgcagtt cgccgtaacg ttcaccgggg ctgatgttac agccgatgga
    10501 acattgctgg cagatgctcg gcgcaaactg catatcccat ttacggttgt aacgctcgga
    10561 gtgcgttttg tcggtaaata cgccggtcgg gcaaatttcg accaggttac cggaaaattc
    10621 gctttccagc gtgccgtctt ccgggcgacc gaagtagacg ttgtcgtgcg cgccataaac
    10681 gcccagatct gtaccgtcag cgtaatcttt gtagtaacgc acacagcggt agcaggcgat
    10741 gcagcggttc atttcgtgag agatgaatgg ccccaaatcc tgattacggt gggtacgttt
    10801 ggtgaaacgg tagcgacgga agctgtgtcc ggtcatcacg gtcatatcct gaagatggca
    10861 gttaccgcct tcttcacata ccggacagtc gtgcgggtgg ttggtcatta accactcgac
    10921 cacactttca cggaactgtt tcgcttcttc gtcgtcaatg gaaataaagg tgccatcgga
    10981 agccggtgtc atacaggaca tcaccaggcg accacgcgtg tcttccgcgt tttggtattg
    11041 cttcaccgca cactggcggc aagcaccgac gcttcccagc gccggatgcc agcaaaagta
    11101 aggaatatca aggcccagag acagacaagc ttccagcagg ttgtccgctc cgttgacctc
    11161 gtattctttg ccgtctacat gaattgtagc cattagcatg cttccagttt tctcggtcag
    11221 ggaccgagcg ttaatcgaaa ttcggttacc agcgctcttt cagcaggttc ggctgaatcc
    11281 cattaatcaa atgggtattg ctgaacggct gtttgattcc cgcctcaaat tcttcgcgga
    11341 aatatttgat ggcgctctgt aacggctcca ctgcaccagg tgcgtgggca cagaaagttt
    11401 tacccgggcc taagaatcga cacagttgct caagtgtttc gatatcgccc ggctggcctt
    11461 cgccacgctc cagcgcacgc agaattttca cgctccacgg cagaccgtcg cggcacggcg
    11521 tacaccagcc gcaggactca cgggcgaaaa actcttccag gttacgcacc agcgacacca
    11581 tgttgatctc atggtcaacc gccatcgcca gcgccgtacc cagacggctg cccgctttac
    11641 cgatactttc gaattccatc ggcagatcaa ggtgcgcttc ggtcaggaag tcagtccccg
    11701 cgccgcctgg ctgccaggct ttaaatttca gaccatcacg cataccaccg gcgtaatctt
    11761 cgaggatctc gcgtgcggtg gtgccgaacg gcagttccca cagtcccgga tttttcaccc
    11821 gaccggagaa gcccatcagc ttggtgccag catctttact tttcgagatg ttctgatacc
    11881 actccacgcc gttagcgagg atcgccggaa cgttacacag ggtttcgacg ttgttgacac
    11941 aggtcggttt gccccatacg ccggaggttg ccgggaaggg gggtttcgag cgtgggttag
    12001 cacgacgccc ttccagggag ttgattaacg ctgtttcttc cccgcagatg tagcgccctg
    12061 ccccggtatg gacgaacagt tcgaagtcga aacctgttcc cataatgttt ttgccaagca
    12121 agcccgcttc agtggcttcg gcaatggcgc ggcgcagatt tactgccgct tcgatatatt
    12181 cgccacgcag gaagatgtag ccacggtaag ctttcagcgc aaacgcggag atgagcatac
    12241 cttccaccag caggtgcggc agttgctcca tcaacaggcg gtctttatag gtgcccggct
    12301 ccatttcatc ggcgttacac agcaggtaac ggatgttcat ggattcgtct ttcggcatca
    12361 ggctccactt caggccagtc gaaaagcccg cgccgccgcg ccctttcaga ccagcgtctt
    12421 ttacctgatt aacgatttcg tccggagaca gcccggtcag cgccttacgc gccccttcgt
    12481 aaccgttttt gctgcggtat tcgtccagcc acactggctg tttgtcatcg cgcagacgcc
    12541 aggtcagcgg atgcgtttcg ggagtacgga taatgttttt catttatacc gctccagcag
    12601 ttcagggatc gcttccgggg tcagatgcgc gtgagtgtcc tcatcgatca tcatgtttgg
    12661 ccctttatca cagttcccca ggcagcaagt tggcagcagc gtaaagcggc catcaaatgt
    12721 cgtttgccct ggtttgatgt tcagcttttt ctcgagcgcc gcctgaatac cctgataccc
    12781 gttgatatga cagaccacgc tgtcgcaata acggatcaca tggcgaccaa ccggctggcg
    12841 gaagatctga ctgtagaacg ttgccacacc ttcgacgtcg cttgccggaa tacccagcac
    12901 atcggcgatc gcgtggatcg caccatccgg cacccagcca cgctgcttct gaacgatttt
    12961 cagcgcttca atggacgccg cacgcgggtc ttcgtagtgg tgcatctcgt gctcaatcgc
    13021 ttcacgctct gccgcactca gctcaaaagc ctcggtttgt ggttgttgat tctcgtgcat
    13081 aattagcggt ccacatctga cataacaaaa tcgatactgc ccagataaac aatcaggtca
    13141 gacaccaggc tgccgcggat cgccgccgga atttgctgca aatgcgcata actcggcgtg
    13201 cggatacgag tgcgataact catggtgcta ccgtcgctgg tcaggtagta actgttgatc
    13261 cccttggtcg cctcaatcat ctggaaagat tcattggcag gcatcaccgg cccccacgac
    13321 acttgcagga agtgggtgat cagggtttcg atatgttgca gcgtgcgctc tttcggcggc
    13381 ggcgtggtca gcgggtgatc cgctttgaac gggccttccg gcatgttgtt gaggcactgc
    13441 tcaagaatgc gcagactctg gcgcagctct tccactttca gcattacgcg ggtgtagcag
    13501 tcagaaacac caccaccaac cgggatttca aagtcgaagt tttcatagcc agaataagga
    13561 cgcgccttac gcacgtcgaa gtcgatcccg gtagcacgca ggcccgcgcc agtggtgccc
    13621 cactccagcg cctctttcgc gccataggcg gcaacgccct gggaacgacc tttcagaatg
    13681 gtattttgca gcgccgcttt ctcgtaagac gccagacgtt tcggcatcca gtcgaggaac
    13741 tcacgcagca gacgatccca gccgcgcggc aggtcgtgcg ctacgccgcc aatacggaac
    13801 cacgccgggt gcatacggaa acccgtgatc gcttccacca gatcgtaaat tttctgacga
    13861 tcggtaaagg cgaagaacac gggcgtcatt gcgccgacgt cctgaataaa ggtcgagatg
    13921 tacagcaggt ggctgttgat acggaacagt tcggagagca taacgcgaat gacgttaacg
    13981 cgatccggca cggtgatccc ggccagtttc tctaccgcca gcacgtaagg catttcgtta
    14041 acgcagccgc cgaggtattc aatacggtcg gtatacggaa tgtagctgtg ccaggactgg
    14101 cgctcgccca ttttctccgc accacggtgg tggtagccga tgtctggtac gcagtcgaca
    14161 atctcttcgc cgtcgagttg taaaacgata cggaaagccc cgtgcgccga cgggtggttc
    14221 ggaccgaggt tgaggaacat gaagtcctca ttttcggtgc cgcgcttcat cccccactct
    14281 tccggtttga aggtcagggc ttccatctcc agatcctgtt tggctttggt cagctcaaac
    14341 ggcgagaatt cggtagcgcg tgccgggtaa tctttacgca gcgggtgacc tttccaggtt
    14401 tgcggcatca tgatgcggcg caggttcggg tgaccgtcga aagtaatgcc aaacagatcc
    14461 caggtttcac gctcatacca gttagcgttc gggaacagtt tggtgaaggt cggtacgtgc
    14521 aggtcgtttt ctgccagcgc caccttcagc atgatgtcgc ggttacgatc gatagaaatc
    14581 agatggtaga aaacggaaaa atccgcggca ggtaaccctt cgcggtgtgt gcgcagacgt
    14641 tcgtccatgc cgtgtaagtc aaacagcatg acgtaaggtt tcggcagttt ctttaagaaa
    14701 tcgccaactt ccagtaattg ttcacgcttg atccacacaa cgggaacccc ggtgcgagtc
    14761 gcctgaacag taaaggcatc cggcccaaaa cggttgcgca gttcgccaat taccggatca
    14821 tcaagatgat cgcgggtctg ccaggcgggt tcttgcgcgg ttaagtcggt catattgttc
    14881 accattgcaa atggtccgtg gtgactgtcg gcgcagtaag cttcgcgaaa tagatttagg
    14941 aataagcgaa gaaaaatccc tttgccgaca ggcgcaaatt aaatctcgtc aggtgtacgc
    15001 aggttggtta cggcaatgcg ttcaccgcgc ttgcgttcgc gctctgattg catattggcg
    15061 cgataaacgc cctgatcgcc aaccacccag gagagcggac gacgttcttt gccgatagat
    15121 tcctgcaaca gcatcagtgc ctgcatgtac gcttcaggac gcggcgggca gcccgggata
    15181 tacacatcaa ccgggatgaa tttatcgacg ccctgcacaa cggaataaat atcgtacata
    15241 ccaccagagt tggcacaggc acccattgag ataacccatt ttggttccag catctggtca
    15301 tacagacgct gaataaccgg tgccattttg gtaaagcagg ttcctgcaac caccatcagg
    15361 tcagcctgac gcggcgaagc acgcaatact tctgcgccaa aacgcgccac gtcatgcacc
    15421 gcggtaaacg aagtcaccat ctcaacgtaa cagcaggaaa gaccgaagtt atacggccaa
    15481 attgagtttt tacgacccca gttaaccatg tcattgagct tgcccataaa cacgtttttg
    15541 ttaacttctt gctccagagg gtcggttacg atctcctgct tttgcagggg gtaacggtcg
    15601 ttctcaccgt tgggatctat gcgggtgagc gtataatcca tcttaatgcc tcgcggttag
    15661 cgttgacgat tagcgatact gttcgtttcc gggttcatac gctcgcggcg tgaacgcgcg
    15721 ggcgtccagt ccagcgcgcc aatacgcacc agataaacca gacctgccag taacacaaaa
    15781 ataaaaattg cagcttccac aaagcctacc cagccgcttt cgcggataga ggttgaccat
    15841 gcgaacagat acagcgcttc aacgtcgaag ataacgaaga acatggccac cagataaaac
    15901 ttggcggaca ggcgtaagcg ggcggagccg accgagtcga taccggattc aaacggcacg
    15961 tttttcgacc tcgcgcgtgc gcgaccgcct aaaaaccaac cgcctaccag catcaggcaa
    16021 cacaggccaa tggcaacgat aagaaagata gcgaatgccc agtgatgagc gatgacttca
    16081 gtggatgttg acatactcat tgcttactca tcaaaagtag cgccagattc actgctcttc
    16141 acggcagatg gacgccacat cgattcatgg ggaggaataa aaaaaacctt acaataactg
    16201 tagaaattct tttatacagc taattgatgt ggtcttttac tcctttctat aaccttttgt
    16261 caactttaac aaaagcttct tcacattagt ttacataata tcaacaccat tagcatttaa
    16321 tgccctttca ccccagatcc ttgacgactc caggataatt agatgttgtt gaatcgtgtc
    16381 cgttgtgaag caatggaaaa aatacgggtc tattttgaca ggaatttgtg tcgattcctc
    16441 cccccaaaag agagtatttt cttgatctgt gacacgcttt tgttattcca taacaaaaac
    16501 gcagcaacaa atttacgtat tttttaacat cattgtagca ggtgattttt ttcaggcgat
    16561 tatttgtgca ttcgggacgt gaatctctgg tgattgaaaa ataaacagtt ttgtacgttc
    16621 tgcactatgc gaggaaggat ttttactaaa aaaaagccgc tggggtttaa aacaccccca
    16681 gcggctcgtt ttttacacta ttgtctcagg aattatctat cgtccgtcga ttactcgata
    16741 tccctttcaa tcaacaatga atcatcccct tccggagcag acattgggct gtattgccac
    16801 ggattatggt agctctccat cgcctgatag atcacctggg ccagctcatt attactggac
    16861 gggtcatagc acagcaaata ttcggtatca ggtagcggcg gtaagccatc tactccactc
    16921 agcacacgca ggtccgggct catcatctca accggccttg ccgtcacgcc aagaccggct
    16981 ttcactgccg cacgaacggc cggaagcgtc gaggcgacat aagccagtcg ccatggaata
    17041 tctactttat taagcgtcgc cagcaccata tcgcgaaacg gacttggatc atccagcaat
    17101 acaagaggga tcggctcgcc tttttgcaat acgtattccg ctgcgcagta ccagtgtgtt
    17161 ggcgaggtac gcaggttcaa tgccttaaac gctgaaggac gatgggtggt taccatcaaa
    17221 tccacttcct gggattccag catttctgcc atataggcat tacgtttaac gcggacatcc
    17281 agcgctaatt tcggataaac cgaactcacg cgatttaaca ggaaaggtaa gatcgtatcg
    17341 gcagattcat ctgaagcacc gatagttaac acgccctgaa gattactgaa cattaatgat
    17401 gagcaggcct catcattaaa acgcaggatt ttcctggcgt aaccaagaag ttgaatgcca
    17461 tgttcagtta acagtttgtt gcgaccgtga cgagcgaaca gttctttccc aacgagttgt
    17521 tccagacgct gcatttgctg acttacggcg gactgagtac gacacacagc ggcagctgcg
    17581 gcagcaaaag tgttcagatc ggcaacagca acaaatgttc tcagcagatc gaggtcgagg
    17641 ttaattatcg gacgatttgc acttatcata tattatcact tactggcggc tcatactgag
    17701 ctggttaatg ctgtgcacac acaaacaagc aattccattt gtaatgtgcc tccctggcag
    17761 tttcatccgg aacccggacg aaagtaaaaa tgcatatgag ttgcactaaa aaagcgactc
    17821 acattgttcc gttataatgc ctgaagtaga tcacagaata tatcttcagg gatcgcatat
    17881 ctattaagtt actcactctt ttctatttat gacatgcgcg tgtttgtata aatgtaaatg
    17941 tgagtccttg ttccactctc gtgcagcatc gctggtcata cgcgaacacg tacaaacagc
    18001 aatggtgaag cggcatcaag agcagggatc cgtcatttat gcgagcattt taccccaaaa
    18061 accttttatt tataagggtc attgcgaatt atctgatgca aagttatgtt atgttaggca
    18121 aagtaatctt ctattattaa taagcacatc aaaacctttt tgaatattaa ataataatta
    18181 attagcatca tcctcattca ttaattccgc ttaacaatag tttcacacct tacccccatt
    18241 gcgacacctc ggaaatcatc acgctgttaa actctccgta atgcggaaat atcatcatcg
    18301 cgtttcatta gtgaattctt aaatgaggca ttttcacaca attatcttac agataaaaaa
    18361 accagacttg caattaagaa ttagaacaat tcactatata acattgcatg taaagcatat
    18421 aagcctcatt atttgtcatt attaagttat taacagcaca atcgagcctt cccctctggc
    18481 aaaatcttat tctgcagacc ttcaaaacac cgtcctgggg gagtacattg ttctaagctg
    18541 acttccacgg cagggagtgg cgataacagc aaaaaaggtc aagattcatg tcccccattg
    18601 aaaaatccag caaattagag aatgtctgtt atgacatccg tggtccggtg ctgaaagaag
    18661 caaaacgcct ggaagaagaa ggtaacaagg tactgaaact gaacatcggc aacccagccc
    18721 cattcggttt tgacgcgcca gatgaaatcc tcgttgacgt gatacgcaac ctgcctaccg
    18781 ctcaagggta ttgcgattcc aaagggcttt actccgcgcg taaagccatc atgcagcact
    18841 accaggctcg tggcatgcgt gatgttaccg tggaagatat ttacatcggc aatggtgtat
    18901 cggagcttat cgttcaggca atgcaggcat tgctgaacag cggggacgaa atgttggttc
    18961 ctgcaccaga ttacccactc tggaccgcgg cggtttcgct ttccagcggt aaagcggtgc
    19021 attatctttg cgatgaatcc tctgactggt tcccggacct cgatgatatt cgcgctaaaa
    19081 ttacgcctcg tacgcgtggg attgttatta tcaacccaaa taacccaacc ggcgcggtat
    19141 attccaaaga gcttttaatg gagattgtgg agattgcacg tcagcataat ctcattatct
    19201 tcgccgatga aatttatgac aaaattctct acgacgatgc tgagcatcac tcaattgcgc
    19261 cgctggcacc tgacctgctg accattacct ttaacggact gtcgaaaacg taccgcgttg
    19321 caggcttccg tcaggggtgg atggtgttga acgggccgaa aaaacacgcc aaaggctaca
    19381 tcgaaggtct ggaaatgctg gcttcaatgc gcctgtgtgc taacgttcct gcgcaacacg
    19441 ccattcagac cgcgctgggt ggttatcaga gcatcagtga atttattacc cctggcggtc
    19501 gtctttatga gcagcgtaac cgcgcgtggg aactgatcaa cgatattccg ggcgtttcct
    19561 gcgtgaaacc tcgtggtgcg ctgtatatgt tcccgaaaat cgacgccaaa cgctttaaca
    19621 ttcatgacga tcagaaaatg gtgctggatt tcctgttgca ggaaaaagtt ctgttggtgc
    19681 aagggacggc attcaactgg ccgtggccgg atcacttccg cattgtcacg ctaccgcgtg
    19741 tcgatgatat cgagctgtct ttgagcaagt tcgcgcgttt cctttctggt tatcatcagc
    19801 tgtaatctta atttcactgc cggagattgc atccggcagt gttatcccgc cacaatgacc
    19861 tgatgatgtc atcatacgta aggtcactat gaaacagagc catttttttg cccatctctc
    19921 ccgcctgaaa ctcattaacc gctggccgct aatgcgcaac gtgcggacgg aaaatgtgtc
    19981 cgaacacagt ttgcaggtag cgatggtcgc ccatgcgctg gcagctatca aaaatcggaa
    20041 atttggcggt aatgtcaacg ccgaacgtat cgctttactg gcgatgtacc acgatgcctc
    20101 agaagtgctc accggcgatc tccctactcc ggtgaaatac ttcaatgcgc aaatcgctca
    20161 ggagtacaag gctattgaaa aaatcgctca gcagaaactg gtcgatatgg ttccggaaga
    20221 actgcgggat atctttgcgc cgttaattga cgagcatgca tatagcgatg aagaaaaatc
    20281 gctgatgaaa caggcagatg cactgtgtgc atatctgaaa tgtctggaag aactcgcggc
    20341 cggaaataat gaattcttgc tggcaaaaac gcgactggaa gcgacgcttg aagcgcgtcg
    20401 cagccaggag atggactact tcatggaagt atttgttccc agcttccatc tttcgctcga
    20461 tgagattagc caggattcac cgctgtaagc agccggagtc tgcgtcgcat cagtcaataa
    20521 gcgccggatg cgacatcagg ctcttgtcaa aacggaaaca gcatcgggat catcaccaca
    20581 caaaccgcca tcacgataat ggtgaacggt acccccaact tcacaaagtc actgaagctg
    20641 taatttcccg gacctaaaac cagggtgtta acaggtgaag acaccggtgt cataaaggcg
    20701 gcggatgctg ccatcgccac gaccatcgca aatggataag gcgacactcc catcgttttg
    20761 gcagcagcca gcgcaatcgg agccatcaac accgccgtcg cggtattaga gataaacagc
    20821 ccaataaccg ccgacaagac aaacaaacac cccagcatca tatgtggccc ataaccgccg
    20881 ccaatatcca tcagcccttt caccgccagc gcgacacctc ccgttttctg taatgccaca
    20941 gcaaacggca tcatcccaac ggtcaaaata atgctcggcc agtgaatgga tttataggcg
    21001 ctttcagcat ctatacagcg gaatttcccc atcagcaggc aggcgatgat agcggcaaca
    21061 ggattaggaa tttcatttgt cagcattaac gccaccatta gcaccagaca gaaaatggca
    21121 tggggtgcct ggctgtgcgc gggcgatgct tcactcacct cttccggtaa gttcagcgct
    21181 acgaagtcgc ggccctgttt ggccagcata ccgatcagtt tccagttacc cacaaccagg
    21241 atgatatcgc ccagcagcag aggctcatcc gccagcgaac cttccagcgc cacgccattg
    21301 cgcttgagcc ccaccacatt cagtccgtag cgggtacgaa aaccaatttc gcgcaccgat
    21361 ttaccaatca gttctgactc aggaattaat gaaatctctg ccatgcccac atcaagggcc
    21421 tggtcagaaa aatactcgcc gcgcagtacc atcggctcca gcaattgctc actacaaaat
    21481 tgccggagat cgacatcagc cgcagacata tcaataagca aaacgtcacg cgcgcgaaat
    21541 tcagaaaccc cattaacgtt cacgataacg cgacgaaaac gccgccagcg ttcaacaccg
    21601 atgacgttag cgccataacg ctcacgtaat ttgagatcat ccagccgttg acctatcatt
    21661 ggcgatccgg ggcgaatagc cagacgtcgc gcacgcccgg tcagtcgata ttcacggata
    21721 agatcgcgaa aggttcgacg cgtccagcct tcgcgctgcg gggtctgggt atcccctttc
    21781 agcatgaaac gcatcactaa catatacaaa atacccagca ccagcacgac caggccaata
    21841 ggtgttacgc taaagaaact gaagccgtga tagccttcac gcagcaattc actgttgact
    21901 accaggttcg gcggcgtcgc caccagcgtc atcatgccgc taatcagccc ggcaaaactt
    21961 aacggcatca tcagacgtga cggcgaagtt tgcatacgca tggcaacgct taacaccacg
    22021 ggaataaaga tagcgacaac gctggttgaa ctcataaacg cgccaagccc cgcgacggtc
    22081 agcatcaaca aaaccaacat tttgatttca ctgttgcccg ccactttgac cagccatgtt
    22141 cccattacgg tggcaacacc ggtacggacc aaaccatcgc caataataaa caaggcggca
    22201 atcaggacaa cgttaggatc agaaaagccg gaaaatactt cggggaccgt cagcgttccg
    22261 cttaatgcaa acgcgacaat aacaaacaaa gcgaccgcat ccatacgcac tctgcccgtc
    22321 gcaaacaaga cgatggcaac ggccagtaat gaaagaaccc aaatcaattc accgttcaca
    22381 acttatcctt gttaattgag ggggatgact tgattctgcc ataaaaaagc cccgacgaga
    22441 cggggctaaa tcatgatcag gtgtttcact gaataataac atcgccattt ggctgtttgg
    22501 tcacagtaat ttgctccaga ctgtggagga ccaaatcggc ctcattcagg cgcggggtat
    22561 ctgccggagc gttaaccgca atgacatggc aacccgccgc caggccagaa agcacgccag
    22621 cgggagcatc ttccaccacc acacactcct gcggcgcaag ccccagcagc tgcgcaccta
    22681 acagatacgc atcaggttct ggttttccgc gcttcactcg ctcagcggtt acaaacacct
    22741 ctggtgcggg aagccccgct attttatggc gcgctcgcgc taccggcatg gagccagaag
    22801 tcacaatggc ccacggaata cctgctttat tcaaatgatt gagtaaggcg attgccccag
    22861 gaagcgcggt aataccttcg gtttccgtgg cctcgatttg ctccagacgc gtaaactcgg
    22921 cggcaatatc agcctcggat ttgcccgcca taaaatggcg caaagaggtg atcgcctgtt
    22981 taccgtgaat gaaagccagc acctcttccg gcgctaaccc atgacgtctg gcccagttgc
    23041 tccacgcccg ttctaccgca ggcagggaat ccaccagcgt tccatcaaga tcaaacagaa
    23101 aacctttgca ccgcacgcgg gcctcctcag gcgttgataa tttgattaat ttcgttggcg
    23161 ctcaaatggt actgacgcgg gcaggcatgc cacacattaa gcatgcgctg atatttttcc
    23221 cacattggcg tctgggcgtt aaaaccatga gtcccagcgt caaaatgggt atagcgccct
    23281 tccacattaa ccataaagcg gacataaccg aggtaacgtg cttcagtggc ggcgtcaaag
    23341 ccgaggaagg tgacacgacg ttcatcgatg gattgctggt cctgcaaatt agaccaggaa
    23401 acatacaacg catgatacat atccataatg tcgatgatag tgcggcaggt ttcttctttc
    23461 agctcgccaa actcgcgatc caattcacgc atctgtaatc cgtaaccacg ctcaataatt
    23521 gtttgcaggc gacggtaacg ttcagcattt gccggatcga gcatagtcat catcttgtac
    23581 tgattagaca aaataagacg ttgcgcgttg gtcatttcca ttgttgactc ctgtatcact
    23641 ctactacggt gaaaaaaaag aaggctgagt atgccttctt ttatatgcgt aatcaggggt
    23701 caattacaaa tcatcaagga aagttttatc cagttgtttg aaggcgcgct taagcgtgtc
    23761 agctaatgcc tggtaatcag gcttgccttc aacgggtgcc aacacctgtc cagactcctg
    23821 caatttaccg cgaacttcat aaaaccagtt aaggattgca gggggtaatg gcgttacaga
    23881 acgcttgccc agccaccaca atccctgcat gggtaaactt aaggcgaaca gtgcagtggc
    23941 aactgccggc ccaagcagac cgcccagggc aatctgccag cagagagtaa atacggcgat
    24001 cggcggcata aaacggatcg cataacgcgt catcttgata acgcgatttt cgacaaagac
    24061 cggagcaagg cgtttttcca gtggccacgt ctttgagtaa tgctgtccgc ggcgaaacaa
    24121 gctaaaaaaa ttaacagaac gattatccgg cgttgacatg cttcacctca acttcacata
    24181 taaagattca aaaatttgtg caaattcaca actcagcggg acaacgttca aaacattttg
    24241 tcttccatac ccactatcag gtatccttta gcagcctgaa ggcctaagta gtacatattc
    24301 attgagtcgt caaattcata tacattatgc cattggctga aaattacgca aaatggcata
    24361 gactcaagat atttcttcca tcatgcaaaa aaaatttgca gtgcatgatg ttaatcataa
    24421 atgtcggtgt catcatgcgc tacgctctat ggctccctga cgttttttta gccacgtatc
    24481 aattataggt acttccatgt cgagtaagtt agtactggtt ctgaactgcg gtagttcttc
    24541 actgaaattt gccatcatcg atgcagtaaa tggtgaagag tacctttctg gtttagccga
    24601 atgtttccac ctgcccgaag cacgtatcaa atggaaaatg gacggcaata aacaggaagc
    24661 ggctttaggt gcaggcgccg ctcacagcga agcgctcaac tttatcgtta atactattct
    24721 ggcacaaaaa ccagaactgt ctgcgcagct gactgctatc ggtcaccgta tcgtacacgg
    24781 cggcgaaaag tataccagct ccgtagtgat cgatgagtct gttattcagg gtatcaaaga
    24841 tgcagcttct tttgcaccgc tgcacaaccc ggctcacctg atcggtatcg aagaagctct
    24901 gaaatctttc ccacagctga aagacaaaaa cgttgctgta tttgacaccg cgttccacca
    24961 gactatgccg gaagagtctt acctctacgc cctgccgtac aacctgtaca aagagcacgg
    25021 catccgtcgt tacggcgcgc acggcaccag ccacttctat gtaacccagg aagcggcaaa
    25081 aatgctgaac aaaccggtag aagaactgaa catcatcacc tgccacctgg gcaacggtgg
    25141 ttccgtttct gctatccgca acggtaaatg cgttgacacc tctatgggtc tgaccccgct
    25201 ggaaggtctg gtcatgggta cccgttctgg tgatatcgat ccggcgatca tcttccacct
    25261 gcacgacacc ctgggcatga gcgttgacgc aatcaacaaa ctgctgacca aagagtctgg
    25321 cctgctgggt ctgaccgaag tgaccagcga ctgccgctat gttgaagaca actacgcgac
    25381 gaaagaagac gcgaagcgcg caatggacgt ttactgccac cgcctggcga aatacatcgg
    25441 tgcctacact gcgctgatgg atggtcgtct ggacgctgtt gcattcactg gtggtatcgg
    25501 tgaaaatgcc gcgatggttc gtgaactgtc tctgggcaaa ctgggcgtgc tgggctttga
    25561 agttgatcat gaacgcaacc tggctgcacg tttcggcaaa tctggtttca tcaacaaaga
    25621 aggtacccgt cctgcggtgg ttatcccaac caacgaagaa ctggttatcg cgcaagacgc
    25681 gagccgcctg actgcctgat ttcacaccgc cagctcagct ggcggtgctg ttttgtaacc
    25741 cgccaaatcg gcggtaacga aagaggataa accgtgtccc gtattattat gctgatccct
    25801 accggaacca gcgtcggtct gaccagcgtc agccttggcg tgatccgtgc aatggaacgc
    25861 aaaggcgttc gtctgagcgt tttcaaacct atcgctcagc cgcgtaccgg tggcgatgcg
    25921 cccgatcaga ctacgactat cgtgcgtgcg aactcttcca ccacgacggc cgctgaaccg
    25981 ctgaaaatga gctacgttga aggtctgctt tccagcaatc agaaagatgt gctgatggaa
    26041 gagatcgtcg caaactacca cgctaacacc aaagacgctg aagtcgttct ggttgaaggt
    26101 ctggtcccga cacgtaagca ccagtttgcc cagtctctga actacgaaat cgctaaaacg
    26161 ctgaatgcgg aaatcgtctt cgttatgtct cagggcactg acaccccgga acagctgaaa
    26221 gagcgtatcg aactgacccg caacagcttc ggtggtgcca aaaacaccaa catcaccggc
    26281 gttatcgtta acaaactgaa cgcaccggtt gatgaacagg gtcgtactcg cccggatctg
    26341 tccgagattt tcgacgactc ttccaaagct aaagtaaaca atgttgatcc ggcgaagctg
    26401 caagaatcta gcccgctgcc ggttctcggc gctgtgccgt ggagctttga cctgatcgcg
    26461 actcgtgcga tcgatatggc tcgccacctg aatgcgacca tcatcaacga aggcgacatc
    26521 aatactcgcc gcgttaaatc cgtcactttc tgcgcacgca gcattccgca catgctggag
    26581 cacttccgtg ccggttctct gctggtgact tccgcagacc gtcctgacgt gctggtggcc
    26641 gcttgcctgg cagccatgaa cggcgtagaa atcggtgccc tgctgctgac tggcggctac
    26701 gaaatggacg cgcgcatttc taaactgtgc gaacgtgctt tcgctaccgg cctgccggta
    26761 tttatggtga acaccaacac ctggcagacc tctctgagcc tgcagagctt caacctggaa
    26821 gttccggttg acgatcacga acgtatcgag aaagttcagg aatacgttgc taactacatc
    26881 aacgctgact ggatcgaatc tctgactgcc acttctgagc gcagccgtcg tctgtctccg
    26941 ccagcgttcc gttatcagct gactgaactg gcgcgcaaag cgggcaaacg tatcgttctg
    27001 ccggaaggtg acgaaccgcg taccgttaaa gcagccgcta tctgtgctga acgtggtatc
    27061 gcaacttgcg tactgctggg taatccggca gagatcaacc gtgttgcagc gtctcagggt
    27121 gtagaactag gtgcaggcat tgaaatcgtt gatccagaag tggttcgcga aagctatgtt
    27181 ggtcgtctgg tcgagctgcg taagaacaaa ggcatgaccg aaaccgttgc ccgcgaacag
    27241 ctggaagaca acgtggtgct cggtacgctg atgctggaac aggatgaagt tgatggtctg
    27301 gtttccggtg ctgttcacac taccgcaaac accatccgtc cgccgctgca gctgatcaaa
    27361 actgcaccgg gcagctccct ggtatcttcc gtgttcttca tgctgctgcc ggaacaggtt
    27421 tacgtttacg gtgactgtgc gatcaacccg gatccgaccg ctgaacagct ggcagaaatc
    27481 gcgattcagt ccgctgattc cgctgcggcc ttcggtatcg aaccgcgcgt tgctatgctc
    27541 tcctactcca ccggtacttc tggtgcaggt agcgacgtag aaaaagttcg cgaagcaact
    27601 cgtctggcgc aggaaaaacg tcctgacctg atgatcgacg gtccgctgca gtacgacgct
    27661 gcggtaatgg ctgacgttgc gaaatccaaa gcgccgaact ctccggttgc aggtcgcgct
    27721 accgtgttca tcttcccgga tctgaacacc ggtaacacca cctacaaagc ggtacagcgt
    27781 tctgccgacc tgatctccat cgggccgatg ctgcagggta tgcgcaaacc ggttaacgac
    27841 ctgtcccgtg gcgcactggt tgacgatatc gtctacacca tcgcgctgac tgcgattcag
    27901 tctgcacagc agcagtaatc tcgtcatcat ccacagcttt gcgctgcgga tatctgaacc
    27961 ggaaataatc actatttccg gttttttatt ctcttaattt gcattaatcc tttctgatta
    28021 tcttgcttaa ctgcgctgca tcaatgaatt gcgccatctc actttgcata cttaccactt
    28081 tgttttgtgc aagggaatat ttgcgctatg tccgcaatca ctgaatccaa accaacaaga
    28141 agatgggcaa tgcccgatac gttggtgatt atcttttttg ttgctatttt aaccagtctt
    28201 gccacctggg tagttccggt ggggatgttt gacagtcagg aagtgcagta tcaggttgat
    28261 ggtcaaacaa aaacacgcaa agtcgtagat ccacactcat ttcgcattct gactaacgaa
    28321 gcaggcgaac ctgagtatca ccgcgtacag ctgttcacga cgggcgatga acgcccgggc
    28381 ctgatgaact tcccgtttga agggttaacc tcaggatcga aatacgggac agccgttggc
    28441 atcatcatgt ttatgctggt gattggcggc gcgtttggca ttgtgatgcg tacaggaacc
    28501 attgataacg gtatcctggc gcttattcgc catacccgcg ggaatgaaat tctctttatt
    28561 cctgcgctgt ttattctgtt ttcacttggc ggcgcggtat ttggtatggg agaagaggcc
    28621 gtcgcctttg ccattatcat cgcaccgcta atggtccggc tgggctatga cagtattacc
    28681 accgtcctgg tgacttatat tgccacgcaa atcggttttg ccagttcgtg gatgaacccg
    28741 ttttgtgtgg ttgttgctca ggggattgcc ggcgttccgg tgctttctgg ctccgggttg
    28801 cgcatcgtgg tgtgggttat cgccactctg attggcctga tctttaccat ggtgtacgcc
    28861 tcacgagtga aaaagaatcc tcttctgtca cgcgtgcatg agtccgaccg cttctttcgt
    28921 gaaaagcagg cggatgttga acaacgtccg tttacctttg gtgactggct ggtattgatt
    28981 gtcctgacag ccgtaatggt ctgggtgatt tggggcgtga tcgttaatgc ctggtttatt
    29041 ccagaaattg ccagccagtt cttcaccatg ggtctggtga ttggcatcat cggcgttgtt
    29101 ttccgcctta acggcatgac ggttaatacc atggcttcat cctttaccga aggggcgcga
    29161 atgatgatcg cccctgccct gctggtgggt ttcgccaaag ggattttgct gctggttggt
    29221 aatggtgaag caggtaatgc cagcgtgtta aatactatcc tcaacagcat tgccaatgcc
    29281 attagcggtc tggacaacgc ggtcgcggcc tggtttatgt tgctcttcca ggctgtattt
    29341 aatttcttcg tgacgtccgg ttctggtcag gcggcattaa ctatgccgtt actggcaccg
    29401 cttggcgatc tggtcggtgt taaccgtcag gttaccgtgc tggcgttcca gtttggtgat
    29461 ggcttcagcc acatcattta cccaacctca gcttcgttaa tggcaacgct cggtgtgtgc
    29521 cgggtggact tccgtaactg gctgaaggtg ggcgcgaccc tgcttggact gctgtttatt
    29581 atgtccagcg tcgtagtgat cggcgctcag ttgatgggct accactaaaa atgttaagag
    29641 ccgcaattgc ggctcttttt tcattctgcc gtttcagtct ctacggcttc atttttggca
    29701 ttgcgcttca tccacaacgc cagcgctttc agcgagtctg gagtgaactc atcgcagcgt
    29761 gcggtgattt cttccggcgt cagccagcaa actccactga cttcatcttc ctgtagggcg
    29821 aagggaccgt gagagacgca actgaacaac gcgccccaga cacggcaatt tttatcttcg
    29881 aaatagaact gcccgtgctc ggcaaagggg acaccggcaa tgcccaactc ttcttccgct
    29941 tcgcgacgcg cggattccag cagttgctca tcggcctgaa ctacaccgcc tgcggtcgca
    30001 tctaacatgc cgggtaaaaa gtcttttgtc tcggtacgac gctggaccag aattttgccc
    30061 atgccatcat gcacgacgat gtaagttgca cgatgacgca gacactgtgc ccgcatttgt
    30121 tcccggctgg cttgtgcaat gacttcgttc tcttcattga caatatccac ccattcagta
    30181 cttgccaaac gacgctgttc cattatcagg aaaccttctt tttctggcgc tcttacggcg
    30241 cattttgagt tgtgggtaaa ttacggatta atcgcgacct gcgcaatgat actttgatca
    30301 ttgagtgcga ttacactaag tacgtcatta tccagcatgc cataacccgc cggattaccg
    30361 cctttcggaa tactcaccga gccggggttg aagtggaaaa tctccccccg ctgttctgcc
    30421 actggcagat gggtatgacc gtacaccagc acatcgttct ggtttaaagc aggtagattt
    30481 tccgggccaa aaagatgacc atgcgtcaaa aacagacgtt gtttttccag taatacctgt
    30541 tgccacggcg cggttatcgg gaaatgcagc atcatttgat ccacttcgct gtcgcagttg
    30601 ccacgcacag cgataacctt atgtgccact tcattaagcc gttcagcgac tttggctggc
    30661 gcgtaaccct ccggtaaagc attacgcggg ccgtgattca acacgtcgcc aaggatcacc
    30721 agccactggg caccgctttg gacaaacaac tccacaacac gttccgtcgc cggtaacgac
    30781 ccatgaatgt ccgatgcaaa catcagtttc atcactcact cctcgtcgaa aaaacgtaag
    30841 ctatgatact ggattctgtc gttgctatca gccagattgc ttcgccgaaa gtgcaataaa
    30901 acgctggaca gacgctcgct gccactggaa tgtcgcataa tccaccaggc gttctggcac
    30961 ctcatcgcca tgtagtacca ggcggttaat cattagcgcc agatcagtat cagcaatgca
    31021 ccattcacca aataaattcg gctgacccag tgctaacaaa tgttctgcca tcgcgaacag
    31081 tttctctgca ctggcttttc cctcggccgt tagtggcgct tttttcgccc ccgcaaagac
    31141 aacatccgtc ggacgctctt cgcggatggg catcagatcg ctgcgcagcc aggcctgaat
    31201 ctgtcgtgca cgcgcacgat tttctaaatc aagcggataa atacgttccc aggtcggtgg
    31261 cgcaaatcga tcttccagat actccgcaat ggcagaagat tcactcaact caaaatcatc
    31321 gatttgtaat aacggcacac ggcgtgtctg accgtaacct tgccacgtcg gctgcaaatg
    31381 ttcaccgctg tcgaggtcga tggtcttgat atgaaacgac aggccttttt cctgcaacgc
    31441 cacccaggcg gataacacat aaggggagaa aaagtgggca tctgaccaaa gcgtgatagc
    31501 gggtttactc attatgtcct cggcaataaa gggggctggc tatcaaacta tagagccttt
    31561 ttgtcactgt cacgtgacga atactcacgt aagtcgccgc gctttctata cttgtggtgt
    31621 tcagatgatt aaacaaccgg agctgcaatg atcgatctct atttcgcccc gacacccaat
    31681 ggtcacaaaa ttacgctgtt tctcgaagaa gcagagcttg attatcgctt gattaaggta
    31741 gacttgggga aaggcggtca gtttcgtccg gaatttttgc gcatttcgcc caacaacaaa
    31801 attccggcaa ttgttgatca ttctcctgcc gatggcggcg aaccgttaag cctctttgag
    31861 tctgctgcca ttttgttgta tctggctgag aaaacaggac tctttttgag tcatgaaacg
    31921 cgtgagcgcg ccgccacatt acagtggtta ttctggcagg taggcggact ggggccgatg
    31981 cttgggcaaa atcatcattt taatcacgca gccccccaaa ccattcctta cgctattgaa
    32041 cgttatcagg ttgaaactca gcgtctttac catgtactaa acaagcggct ggaaaactcg
    32101 ccctggctgg gaggcgagaa ctacagcatt gcggatattg cctgctggcc gtgggttaat
    32161 gcctggactc gcctgcgaat tgacctcgca atgtatccgg cagtcaagaa ctggcatgag
    32221 cggatccgtt cgcgccctgc caccgggcag gcactgctaa aagcacaact cggtgatgag
    32281 cgttcggata gttaacagaa acaggttctc gtgtattatt tcatcctaag taaaacaacg
    32341 gagaacctgc aatggcacaa cctgccgcta ttattcgtat aaagaacctt cgtttgcgta
    32401 cgtttatcgg aattaaggaa gaagaaatta acaaccgtca ggatattgtt atcaatgtga
    32461 cgatccacta ccccgccgat aaagcgcgca ctagcgaaga tatcaacgat gcgctgaatt
    32521 atcgcaccgt aacgaaaaac attattcagc atgtagagaa taaccgtttc tctttgctgg
    32581 aaaaattaac tcaggatgtg ctcgatatcg cacgtgaaca tcactgggtg acgtatgctg
    32641 aagtggagat cgataaactg cacgcgctgc gctacgccga ttcggtatcc atgaccttaa
    32701 gctggcagcg ttaatcgcca tatcgggagg ctgcatgaac atagtgatca ccggagggac
    32761 gggattaatt ggtcgccatt tgattccgcg tttgctggag ctgggccatc aaattacggt
    32821 agtgacgcgt aacccgcaga aagccagttc cgttctcggc cctcgggtga cactatggca
    32881 agggcttgcc gatcaaagca acctcaacgg cgttgatgcg gtaatcaacc tggccggaga
    32941 accgattgct gataaacgct ggactcacaa gcaaaaagag cgtctctgcc aaagccgctg
    33001 gaatatcacg caaaaactgg tcgatttgat taatgctagc gacacgccgc cgtcggtact
    33061 catttccggc tcggcaacgg gctattatgg cgacttaggt gaagtggtgg ttaccgaaga
    33121 ggaaccgccg cataacgaat ttactcataa actctgcgcc cgctgggaag agattgcctg
    33181 ccgggcgcaa agtgacaaaa cgcgagtgtg cctgctgcga accggcgtgg tactggcacc
    33241 ggacggcggt attctcggta aaatgctgcc accgtttcgt cttggcctgg gcgggccgat
    33301 tggttccggt cggcagtatc tggcctggat tcatattgat gatatggtca acggcattct
    33361 ctggctgctg gataacgagc tgcgcgggcc atttaatatg gtttcgccct acccggtacg
    33421 caatgaacaa tttgcccatg cgctcggtca tgcgctgcat cgcccggcta ttttgcgcgt
    33481 ccccacaacc gccattcggc tgttaatggg cgaatcttca gtactggtat taggtggaca
    33541 acgcgcgctg cctaaacggc tggaagaagc gggttttgcg tttcgctggt acgatttaga
    33601 agaggcgctg gcggatgtcg ttcgctgatg tggtttacag caaacatccg ccagttaact
    33661 ccctgtgtta caggattagt ggctttgcgc gataagatcg tctggtgaaa gtcgggtcac
    33721 catcataact aactctctgt ctaaacctct atccagcatc tcctgagcaa tacgcagggc
    33781 ttcttcgtgt ttgccctgca ttgcgccttc ttcacgtaat ctgtcagcaa tggtcatcag
    33841 tttctccttt tcttgtggtg cgcgttctgt tatctcacca ataaatgcac ggaaacgctg
    33901 ggcatcccct gtttgtaata cgtaattaaa cagggctttt agctgtctgt cattagtgtt
    33961 ccctgtaact agcagcgaaa caatttggtc gactaatccc aacagatcgc gctgacgaat
    34021 atgtttctga attaactcca acagcgccat tttgcggtgt tgcataatct cgtcatccgg
    34081 caccacggta atatccacca acggaaacgc tgatgaatat attttgcggg ctatagcagg
    34141 ctcggcaaat tcatcaagcc agcagagtga ataaggataa ggacttctgc aaccatgata
    34201 aaacagcatc gggatcacca atggaagctc tttatagccc gcatcaagat ggttttgcat
    34261 tgccgcaatg gaataacgca tcatgcgaaa agccattaat tcttccggct tactttggtg
    34321 ctctatcact acataaatat aacccactcc ctcctgcgtt ttcacagacc acaagaggtc
    34381 ggaataatat tgccgcaggt cttcatcaat aaaactgttt ggttctagtt taagcgtcgt
    34441 taaatcacac agtttgcgca gcggcgcggg aagatgaata ttaataaaat tccgcgcggt
    34501 gtctggatgg cgtaaaaaag atttaaatac cgcatcatgc ggcgtggaag ttgtcgatat
    34561 tgtcatggcg ttccgtcacc cctcaaattt aagatgacgc gacaataacc acgattaaca
    34621 cccaatgcag caggcagttt ttatctttgg acagcgaatt cagaagtatt tttagcggac
    34681 tgcaactttt acatttcagc ggaagctgac tcgcaaattc cccttgaaaa tactccggca
    34741 gcgttagcca ccggagagga aattatttca gcgatccctt aaggaactgt tgcagacgag
    34801 ggctttgcgg gttgccaaat aactgctccg gcgcgccctc ttcttctatt ttcccctgat
    34861 ggaggaaaat gacgtgagta gaaacatggc gagcaaagcc catttcgtga gtcactacca
    34921 ccatggtttt cccctcttct gccagttgct gcataatacg taacacttcg cctaccagtt
    34981 caggatcgag cgccgaggta ggttcatcaa acagtaaaac ttctggttcc atcgccagcg
    35041 cccgcgcgat agaaacacgc tgttgctgac cgccggaaag atgcaccgga tatttcccct
    35101 gcgcacgttc gtctatcccg actttcgcca gatacttcac cgcccgctcg cgcgcttcct
    35161 gcttgctcag gcccaacacc tgaatcggcg cttccatgac gttttccaac accgtcatat
    35221 ggctccagag attgaagtgc tggaatacca tcgtcaggcg tgtgcgcagt aagcgcagtt
    35281 gatttttatc ggcgactttg agttgaccgt ctttgtcgcg caccagattg atcgtctggc
    35341 cattgaccac gatcgatcct tcactcggtt tttcgaggaa gttaatgcag cgcagaaagg
    35401 tacttttccc cgatcccgac gatccgatga tgcttattac atctccggca ttcgcttgca
    35461 gtgagacccc tttcagcact tcgtgttcgc catagcgttt gtgcaaatcg ataacgttta
    35521 atttattctc ggacatcgta ttctcagtgc gttgaagaag gtttcacatg ctgcaaccag
    35581 cgtttttccg ctctgcgaaa gaggctgatc aggacataag agatgattaa atagagcacc
    35641 gcggcaatgc cgaaggcggt aaaaggttga tacgtggcgg cgttaatatc gcgggctatt
    35701 ttcagcagat ccggcacagt ggccgtaaac gccagcgcag tagagtgcag catcaggatc
    35761 acttcgttgc tgtatgccgg taacgcaata cgcagcgcag aaggcaaaat aatgcagcga
    35821 tacattttaa aggtcgagaa gccataggct ctggcggctt caatttcccc atgcggaacc
    35881 gaacggattg ccccagcaaa aatctcggta gtgtaagcgc aggtgttaag cgtcagcgcc
    35941 agcacggtac agttcaggcc actgcggaag aaagcgttaa ggaattcggt tcccttaaca
    36001 atctcaagcg tgtacatgcc ggaatagaac accagcaact gaacatacag cggcgtaccg
    36061 cgaaaaatat aggtaaataa ccagattgga aactggatgt atttattact ggagacacga
    36121 ccaatcgcca gaaacagcgc caggactccg cctatcacta ccgacaaaat aagcagccac
    36181 agagtgatcg ccacaccagt aaagcgataa ccgtcggtcc acagcagcgg tttccagtat
    36241 tcatgtaaga tttcgatcac aggtcagccc tcttcacacc cacggagtag cggcgctcaa
    36301 ggaacagcag cacaccattg gaaacggtgg tgaaaaccag gtaaatcacg ccacagacga
    36361 tggcgaaata gaacggttcc caggtacttt tgcctgccag ctgcgtggct ttgaccacat
    36421 cttccaggcc gagtaacgaa accagtgcgg tagatttgag gatcacctgc cagttgttgc
    36481 caatgcctgg cagggcgtaa cgcatcatcg ccggaaacat gatccgccga aacacttgcc
    36541 cgcgagtaaa accgaacgcc gtcgccgcct ctatatgccc tttcggcact gccataaacg
    36601 cgccacggaa cgtttcggta aaataagcac cgtagataaa accgagagtg ataataccag
    36661 cgaccatcgg atcgatatca atctgcccga cgcccatcgc ctccgtcacc atgtttagcg
    36721 caatctgcaa accatagaaa atcagcagca tcaacaccaa gtccggcacg cctcgaatca
    36781 gcgtggtgta cccttcgaaa atcagccccg aaagccgatt ttgcgagagc ttaccgccag
    36841 cgccaattaa accgatgatt acagcgagca ctacagagct gatagccagc tccagcgtga
    36901 cgagcgcacc ctgtaaaata acacctgaaa acccatacaa catgctgcct gtcctgtcgt
    36961 gtgtggtgaa ttaccgcctc ttgtctccct ccggttaccc gaagggagcg ggctttcttt
    37021 caccgatggc gattaaccac cataaacatc aaaatcgaag tactttttcg ctaatttctc
    37081 gtaagtaccg tcagcgcgca tttcggcaaa ggctttgttc agtgcctcgc gcagttcgtt
    37141 atcttcttta cgcaggccca tgccggtccc tacgccaaac agtttttcat ctttaacaga
    37201 cggaccaccg aatttgtaat ctttaccgac gggttgtttg aggaaacctt cgctggcagc
    37261 gacctcatcc tggaacgcgg catcaatacg tccggcagtc aggtcagaat aaatgttgtc
    37321 ctgcccctga tacgagacga tttcaatgcc ttttggtgcc cagtgttcat taccgaacgt
    37381 ctcctgggtg gtgccctgca atacgcctac ccgtttgcct ttcagcgact cgactgtcgg
    37441 ctgaatgtca gaatttttcg ccaccaccag acgagaatcg gcagcgtaca gtttgtcggt
    37501 gaaggcaatt tcttgctgac gtttttccgt aatggaaagc gatgacatga tggcgtcaat
    37561 cttcttcgct tttaaggacg ggattaacgc atccagcggg ttttcgacaa acgtacattg
    37621 cgtattgatg cgcttgcaca actccttcgc cagctcgata tcgaaaccaa ccagttcgcc
    37681 ttgtgaattt tttgattcaa atggcgcata ggtcgggtcg gtaccgatgc ggatgttttg
    37741 cggaattgca gcaagtgccg cagttgcgct ggagaaggcc agaaccagag agagcgatag
    37801 caccagtttt ttcataatgt cctcaactga cagtcttttt atagggaata tttacaggtt
    37861 tgttacactt atcgtgccat aaaattgacc atcaaggcaa aatatcccgc cccgttagac
    37921 gtattatttt tcattaaaaa tgcttaagta tgcatttcaa tgcaccacga tagtgcaaca
    37981 aacctggcgt gcaccatgat gatcattcca tcaggtacag cttcccagcg acgtatcagt
    38041 caccgtagac attaaagtcg aaatactttt tcgccatctt gtcgtaggtg ccgtcctgac
    38101 gcagctcgcc aagcgcctta ttgaaggcag ccgtcagttc agcataatct ttacgtagcc
    38161 ctacaccggt gccatcaccg aagtattttt tgtcttttac tgatgagcca gcaaaagcga
    38221 aatctttacc agcaggttgc ttgaggaatc cttcgctggc agcaacttca tcttgtaacg
    38281 cagcatccag acgtcctgca gccagatcgg aatagaccaa atcctggttg gcataggcca
    38341 ccacatccac gcctttacta cgccaggtct cgttagcgta agcttcctgg gttgatccct
    38401 gcagcacacc aacatgttta cctttcagtg aatccagcgt tggctgaatc ggtgaacctt
    38461 tggcagcaat caaacgagaa tctgcggcgt acagcttgtc ggagaaggca atctcctgct
    38521 gacgtttatc ggtaatggaa agcgacgaaa taatagcgtc gatttttttc gctttcagtg
    38581 aggggatcag cgcgtcaaag tcactggcaa cccaggtaca tttcacctgc atccgtttgc
    38641 acatctcgtt accgagatcg atatcaaagc caacaaaatc acctttagca tctttcgacg
    38701 agaacagcgc ataggtggta tcggttccga tacgtaccgt ctccggtagc gccgcatagc
    38761 tggaagccgc tgcggagaga ccgactaata aagacagagc gagaatcgac ttcttcatac
    38821 ataaccctca agtgaaaatg gcttttttat gttgtgtatt gtgttgtgtg tttgcaggct
    38881 ctttcatgca ggtcttatgc catcttgacg acaacagtaa cattcaacgt taaatatgtt
    38941 aataagacat tgcattattg tcctgaagtt gaagatagca ggtatagcgg ttgaatcgca
    39001 gcgtttcgat tgaatggcag aaacaaaatg tcgaggattt gatcgcggtt gcaaaattgc
    39061 cctgaaacag ggcaacagcg gagttatgcg ccctgccagc gggcaaagag atcttcagga
    39121 agggttattg caaactggtc aagaacacga ttaaccgtct gatttatcac atcatcaagg
    39181 gattgcgggc gatgataaaa cgccggaacg ggaggcataa tcaccgcacc gatttctgcc
    39241 gcctgagtca ttaaacgcag atggcctaag tgcaatggtg tttcacgcac gcagagcacc
    39301 aacgggcgac gctctttcag caccacatct gccgcacggg tcagtaagcc atcagtatag
    39361 ctatggacaa tgccggacag ggttttgatt gaacagggta aaatcaccat ccccagcgtc
    39421 tggaaagaac cggaagagat gctggcggaa atatcgcgcg catcgagcgt gacatcagct
    39481 aatgcctgca cttcgcgcag ggaaaaatcc gtttcgaggg ataaggtctg gcgcgccgcc
    39541 tggctcatca ccagatgcgt ttcgatatct gtgacatcgc gcagaacctg taataagcgc
    39601 acgccataaa tcgcgccgct ggcaccgctg atgcctacaa tgagtcgttt cataaatagt
    39661 tgccctgtca gacttgcagg cagactttgc agtatttcgg cgggagttgc aagtcagggt
    39721 gccagaccgg caccctcagc gaaggcatca tccttcgtta tgcatttcga gattttccac
    39781 ttcgttctga cgttgcactg ctttggcgtc atcattacgt aacgtatcga ggaaatcgag
    39841 gtagccctga tcaacatctt tggtgacgta gacgccgttg aacaccgagc attcaaactg
    39901 ctggatatcc ggattttcag cgcgaacggc gtcgatcaga tcgttcagat cctggaaaat
    39961 caacccgtca gcaccgatga tctggcgaat ttcatcaact tcgcgaccgt gagcgatcag
    40021 ttccgtggcg ctcggcatat caataccata aacgttcggg aagcgaattt ccggtgccgc
    40081 agaagcgagg tacactttct tcgctccggc ttcgcgtgcc atctcgataa tctgctcaga
    40141 agtggtgcca cggacgatgg agtcgtcgac cagcaggacg tttttatcgc ggaactcggc
    40201 gcggttggca ttcagtttac ggcgcacgga cttacgacgc agctgctggc ccggcatgat
    40261 aaaggtgcgg ccaacatagc ggtttttaac gaagccctgg cggtacggtt tgcccagaat
    40321 acgagcaatt tccagcgcga tatcacacga ggtttctggg atcgggatca ccacgtcgat
    40381 atccagatct tcccattcgc gggcaatttt ctcgcccagt ttcgtgccca tattcacacg
    40441 cgcgctgtaa acggaaattt tgtcgataaa cgagtccggg cgggcaaagt atacatactc
    40501 aaacaggcac ggattgctga ccggattgtc agcacattga cgggtaaaca actgcccttc
    40561 ttcagtgatg taaatcgctt cgcccggcgc gacgtcacgc aggaaatcaa agcccagcgt
    40621 atcgagcgct acgctttcgg aagcgaccat atattctgta cggttctcgt caatatcacg
    40681 ttttcccagt accagcggac gaatcccgtt tggatcgcgg aaagcaacca taccgtggcc
    40741 gataatcatc gccacacagg catacgcgcc gcggattaag cggtttgtgg cagcaatggc
    40801 agcgaaaata ttgtcggctt ccagcgggta gtggcggaag ttgtccagct cgctggcgaa
    40861 gatattaagc agaatttccg agtcggaagt ggtgttgatg tggcggcgtt tttcttcaaa
    40921 cagtttttta cgcaactcgt gagcgttggt cagattgccg ttgtgggcaa gcgtaatgcc
    40981 atacggggag ttaacgtaaa acggctgcgc ttcagaggcg ctggagctgc cagccgtagg
    41041 gtaacgcaca tgaccaatgc ccatattgcc ctgcaaacgc tgcatatggc gagcttcaaa
    41101 tacatcgctc accagcccgt tcgctttacg caaacggaag cagttattgg catctatggt
    41161 gatgatgccg gcggcatcct gaccgcgatg ctgaagcacc gttaaggcat cataaatcga
    41221 ctggttaacc ggcataacac cggcgatacc gacaataccg cacatacgtc tttttcctcg
    41281 ttaagccaca tctcagagca cttacgctct gggcaagaaa cttgacgagc tttgcagata
    41341 atcaaaaaac catctgatga taaaactgaa ttgcgggatc agctgtgatt tgctccagtc
    41401 ttcacttttc gacaccccgg taaaggagtc gagaaagaag agaatggcgg caacaatcaa
    41461 cacaccgcgc aacgcaccga aacagacgcc cagcacccga tcggtgcctg acaaccccgt
    41521 tttctccacc aactggccta tcacgaagtt cacgatagca ccaacgatca gggtagcgat
    41581 aaacagtacc gcgatggcaa tcccatttcg aaccagttcg tcttcaaagc ccgtaaacca
    41641 gactgacagg taagtgtagt aatgactggc aacaaagaaa gcacaacccc atgtcaccag
    41701 cgataacgct tcacgaacaa agccgtggat caggctaacc agagaggaaa aagcaatcac
    41761 cgcgattatg gcgtaatcaa tccagaccat atgtgtccca cgattttacg ccctgtcatc
    41821 ctgttcgggg cgcattctaa cagaaaaaga aaacgtttgc gtagggattt ccttcccgcg
    41881 catcaataaa aatggcgctg aaaaaatatt caacgccatc gactttttat gcctttgcgg
    41941 catcgtgcaa tgcgtgtcgg atgcggcgta aacgccttat ccgacctacg gttctaacac
    42001 tgcccaagcc tgataagacg cgccagcgtc gcatcaggca agactgtatt aattcggcgt
    42061 atagcccatt accacgccac ttaagccaga aagttgcttc aactcaccca gcgaaccttt
    42121 cagcttatct ttcgaggcat ccggcccaac cagaatacgg gtaattttac cctgcactgg
    42181 cgtggatggc gacgtataaa cccgataacc ggcaccgcgc agcttaccga caatctcatt
    42241 cactttatcg gcatttttca gcgcacccag ttgcacaaca taggctttac ccgttggcgc
    42301 tgctttttct tccacgaccg gcttcggttc tggcttcggc gcaggtggta cttcaacctt
    42361 tggtttaggc ggctccaccg gtttcggctt cggtggggcg accggtgcag gttccggttc
    42421 aaactcggtg ttattggctg caatagtggc cggatcgagc gacggtgctg cggcatcacc
    42481 tgcccgcacc tcttccgctg cgccttccgg cggctgcgtc ggcaacgcct gggtggcggc
    42541 tggcatcata tcaggctcat cacgatcgcc cgctttcggc accagcggga tagccgcgaa
    42601 ctcatcctga taatgttttt tctgcccgtc cagcagccct ggaagtacaa tcacccccag
    42661 cgccaccagc acgatcgtgc ccactaaccg attctgaaac ttacttgcca ccgcttctcc
    42721 tcgcgtcaat cacttccatg acatgtgcga ccgtgtggaa agaaccacac accagcacgg
    42781 tgtcttccgc tttagcgtcc gccattgcgg catcccatgc ctgcgcaacg ctatcaaatg
    42841 atttgccgtt acccaaatgc tcaagcagtt gttctgccgt ggcaccgcgc ggcccttcca
    42901 gtggcgcaca ataccagtca tcaaccacgc ttttcaacca ggccagagtt ccggcaatat
    42961 ctttatcatg tagcataccg ataaccgcca gcacgcgccc gtttttcggt agcgctttca
    43021 tacgcccggt gagatattcc gccgcatgtg gattatgcgc gacatcaaaa ataacgcgtg
    43081 gcgactcgct cacaatctgg aaacgtcccg gcaaaattgc gctggcaatc ccgtcgcgaa
    43141 tggcattttc actgacttcc agcccgctgg cacgcagtgc cgccagcgcc gttgcggcat
    43201 tcggttgcgg gacaagcggc aacggcagat tttccagcgt gccgtgagca tcgctaaacg
    43261 cccagtcatg atcggtgacg gaatagttcc actcaacgcc ccgacgttgt aacagtgcac
    43321 ctttttcctg cgccacatca gcaatggtag aaggcatttc cggctcaccg acaattgccg
    43381 gtttttcgct gcggaagatg cctgctttct cgagaccaat actttcgcga tctggaccca
    43441 gccagtcggt atgatccagc gcaatactgg ttactaccgc gacatcggca tcgacaatat
    43501 tggttgcgtc cagacgaccg cccagcccta cttccagaat caccacgtca agttgtgcct
    43561 gcttgaacag ccacaacgcc gacagcgtac cgtactcgaa ataggtcagg gaaatatcac
    43621 cgcgtgccga ttcaatctcc gcaaaagagg cggtgtgggc cgattccggc aattcctggc
    43681 cctgcacacg tacgcgctcg gtataacgca ccagatgagg cgaactgtag acgcccactt
    43741 tgtaccctgc cgccatcaga atcgactcca gcgtacggca ggtggtgcct ttgccattcg
    43801 tacccgcaac ggtaaacaca aatggcgctg gtttcaggac gccaagacgc gccgcgacct
    43861 ggctcacgcg ctcaaggccg agatcgatag ttttactgtg caggttttcc agataagaaa
    43921 gccacgaagc cagaggcgac gcggcttgag gagtgcgttt gataatcatg gtatccgctg
    43981 attcgttacg gtgagaatag caaaagggca gagccagtgg ccctgccctt atcagttatc
    44041 aggcctcagg ttcctgatcc ggtaccgggg gtaccactac gccttcacgc ggcgcttcag
    44101 gattcggcgc tggcagattc atcaacttcg ccagaatgct cgccagtttc aggcgcattt
    44161 ccggacgacg gacgatcatg tcgatcgcgc ctttctcgat caggaattca ctgcgctgga
    44221 atctaggcgg cagtttttcg cgaacggtct gttcgataac acgcggaccg gcaaagccga
    44281 ttaacgcttt aggttcagcg atgttgagat cgcccagcat ggcgaaactt gcagaaacac
    44341 cgcccatcgt cgggtcggtc agcacggaga tgtacggcaa gccgcgctcc tgcatttttg
    44401 ccagtgccgc agaggttttc gccatctgca tcagcgacat cagtgcttcc tgcatacgtg
    44461 cgccaccaga ggcggagaag cagatcagcg ggcagttatc ttcctgcgcc tgctcaacgg
    44521 cacgcacgaa acgtgcaccc acaacagacc ccattgaacc gcccataaag gcgaactcga
    44581 atgccgcagc gacaaccggc attccataca gagtgccttt catcaccacc agcgcatctt
    44641 tttcgccggt ttctttctgc gcagatgcca gacggtcttt atacttcttg gagtcacgaa
    44701 acttcagctc atctttcggc tcaagctcgc tacccagctc cacaaggctt ccttcatcta
    44761 acaggctatg caggcgatta cgcgctgtca tacgcatgtg atggtcacac ttcggacaga
    44821 cctcaagatt acgttccagc tcagcgcggt ataaaacctg accgcagcta tcacacttag
    44881 tccacacccc ttcaggaatg ctcgccttgc gggtgggagt aatgttgctt ttaattcgtt
    44941 caatccagct cattagggac ctttctgtct gaacctggtt cgatgccagt tttatctttg
    45001 gggacgcata atgccatttt tgcccccaac agaccatgaa tgttgcacat taaaacataa
    45061 cagcccgaaa ctttggataa aaaagtggtc gaaccgcgga gttacttttt attttgcggc
    45121 gcgtgccgca gcgcgtttgt gacggattat ttcgatgacg cccggcaaaa tagaaaccac
    45181 aataatcccg acgatcagca gcttaaggtt atcctgaacc atcgggattg taccgaagaa
    45241 atagcctgcg taggtaaaaa gcagtaccca caacagtgcg ccgatcacgt tataagcagc
    45301 gaaatgacgg tacgacatgt ggcccattcc cgcaacaaac ggggcgaacg ttctgacgat
    45361 cggcacaaaa cgggcgagaa taatcgtttt gccgccatgt ttctcataaa actgatgggt
    45421 tttgtcgaga taactgcgac ggaaaatttt cgaattggga ttactgaata acttttcacc
    45481 gaacaaccgc ccgatagtgt aattgaccgc gtcacccaca atcgcggcga tcagcatcag
    45541 cactaccatc atatggacgt tgagatcgtt ggtttccagt gatgccaacg ctccggcaac
    45601 aaacagcaat gaatcacccg gtaaaaacgg cattaccacc agaccggttt cacagaacaa
    45661 aattaagaac aaaatggcat aaacccagac gccgtactcc gcgactagct ctgccagatg
    45721 cacgtcaatg tgcaggataa aatcgatgag gaaataaatc aggtccatat ttgcctatgc
    45781 cttgtactcg tcatctttca ggctgtaact acgttggcta cagcctgaaa tgttccgagc
    45841 attattctcg ttagtccgcc agaaatagcg ggcccattgg cggttttgga agatcatacc
    45901 ggtcagggta atccaccgcg accagataca gcccttccgc ttttgccgtt gctgccgcca
    45961 gcgttctgtc ctttgccgcc agcaactctg ctatccagct ctccggctgg ttgtgggcac
    46021 cgacttccat caggctgccg acaatattcc tgaccatatg atgtacaaag gcattcgctt
    46081 tgatatcaac caccacataa ggaccgtgac gcgtgacatt aatatgcata acgttgcgcc
    46141 acggggttcg ggactggcac tgcaccgcac ggaacgaggt gaaatcattc tcgcccagca
    46201 agcattgcgc agcccgatgc atccgttcag cgtccagcgg ttcgtaaaaa tgggttaccc
    46261 ctttactcag taccgccggg cgcagccgat gattgtagat gatgtagcga taacggcgag
    46321 ccgtggcgct aaatcgggca tgaaaatcat caggtacagc tttaacccaa cgcacggcga
    46381 tgtcaccagg taaattcgca tttacgccca aggtccacgc cgcgtctttg cgctgcgcgg
    46441 ttgtttcgaa atgcaccact tgcccggtac cgtgtacccc tgcgtcagta cgcccggcgc
    46501 agaagacggt gatgggttcg ttcgccacct gggagagcgc cttttccagc ttctcctgca
    46561 cactgcggac ttcattctgc cgttgccagc cgtaatactt actgccgtcg tactcaatgc
    46621 ccagcgcaat tttataaact ggcagttgtt gctggtcgga cattagtaca gatactcctg
    46681 caccagtttc tcggcgattt ttactgccat cagcgcgccg ccaaagcgaa cgttatcggc
    46741 caccgaccag aactggactt gctccggcat accgtagtca ttacgcacgc agccaacaga
    46801 aagatgcggc gtacccgaag catcccctac ctgagtcggg aattcgttct cttcagagag
    46861 cacaatatct tcgccctgag caaacgcatc acgcgcttct tctgccgcca gcggacgcag
    46921 tgcttcaaag ttgaccatct gggcatgacc gtagaatacc ggtgcctgga cgacgcttgc
    46981 cgaaatcatc agcccttcgt cctgcaggat tttgcgtact tcgtcaacga tacgacgttc
    47041 ttcacgcacg ctaccttcgc tatccggcaa caacggcagc atattgaacg ccagttgacg
    47101 accgaagaaa tcttcttcgt caatcggaat gccgttgagc aatttcgcac tctgccccgc
    47161 taacgcatcg accgcttttt tgccctgggc ggaggctgaa atcaggctgg taacactgat
    47221 acgcgacaaa ccgccctgat cgattaacgg tttcagtgcc gccagcagct ggctggtcag
    47281 actgtctggt acggcgatga cattccggtt gcggtaatcg gtcagtacaa acgggtttac
    47341 ttccggcacc accagcggta cgtcgggttc gagagcaaac agtccgctgc tgtcgatcac
    47401 caggcaacct gagttggtcg cttcttcaac ccaggcagcg gtagcttctt tgcctgcgac
    47461 aaaaaatgcc agctgcgcct gcgtccagtc gaattcagcg gcatcctgca cggtgattgt
    47521 tttaccacca aagcgcagtt gttcgcctgc gctttcgtta cgtgccagtg cataaatttc
    47581 cccaaccggg aactgacgtt cagccagcgt ttcaagcagg gcttcgccca tagcgccagt
    47641 tgcgcccagg acggcaatgt tccagccttc agacatggtg gtttactcca gaaatagcaa
    47701 agctccctgc caatgttaca gcagagagca tgaagaagag attaacgtgc cggatgatga
    47761 acggcgttaa aacccagttt acacagcaat gatgccgcac tggcgtcatc acaaattaca
    47821 tacagagatg accattcacg gcgctcaaga tagtttttgc gcagtttatc gaactcaccc
    47881 ggtatcccgg cgactttacg cagcggtgcg tcatcgcggc gcacatcata caccaaatgc
    47941 accagccttt tcagcgtcgg ttgatcgagc ggaccatgca gcgtaatgcg gccaaactca
    48001 ggcgcgggca gcaacgtatc cagcgcaacg tgttgttcat gcccaataaa cttgctatag
    48061 gcttcaaaca cttgcgtcgt gccacgcgct ttaccctcca gggtatagcc tgcgatatgc
    48121 ggcgtgccga tatccacttt tgtcagcaat tccacattaa gttccggctc gccttcccag
    48181 acatccagca ccacgcttaa cttctggcct tcattcaggc aggtcagcag cgcggtatta
    48241 tcgacgactg cgccacggca ggcgttaatc agaatcgctc cgggcttcag gctacggatc
    48301 agtttttcat ccgccagatg tagcgttttg tacggaccat ctttaaagag tggcgtatgg
    48361 aaagtcagaa tatccgcgcg ctggactaac tcatccagcg agcggaaatc accctcatcc
    48421 ccacggtcgg cgcgaggcgg atcgcaaagt aaggttttaa tccctaacgc ttccagtcgc
    48481 gcctgcaatc gacgtccaac gttaccaacg cccacgatcc ccaccgtacg gtcgtgcagt
    48541 gaaaatccat cgcgttcggc aagcatcagc agggaggaga aaacatattc caccaccgca
    48601 atcgcattac agccaggtgc agcggaaaaa ccaattcccg cctgcttcaa ccatgcttca
    48661 tcgacatggt cggtccccgc agtggcagtt ccaacaaatt taatgggttt tcctgccagc
    48721 aaagattcat tcactttcgt gaccgaacgc accatcagcg catccgcgtc tgccagttga
    48781 gcgacgggga ttggacgccc gggaaccgcg atcacctcac ccaaacggct aaataagtcg
    48841 cgggcataag gcatattttc atcaacaagg attttcacgt ttgtgttacc tgtatgagac
    48901 gagagttaac cggacaagta tgccataatc tcgcggccag gcatacttgc gaagatttca
    48961 ggtataagga tacgtaatga tacaacctat ttccggccct cctcctgggc agccaccagg
    49021 tcagggagat aacctgccgt ctggcgcggg caatcagcct ttatccagtc agcaacgtac
    49081 ttcgctggaa agcttaatga cgaaagtgac ctcactgacg caacaacaaa gagcagaact
    49141 gtgggcgggt atcaggcacg atattggtct gtcgggagat tcaccgctgc tttcgcgtca
    49201 cttccctgcc gctgagcata atctggcgca acgtctgctg gccgcgcaaa aaagccattc
    49261 tgcccgccag cttttagcgc aattagggga gtatttacgt ctggggaatc atcgtcaggc
    49321 ggtcacggat tatatccgtc ataactttgg tcagacgccg ctgaatcagc tctcaccgga
    49381 gcaattaaaa accattctca ccctgttgca ggaagggaag atggttattc cgcaaccaca
    49441 gcagcgcgag gcgaccgacc gtcctttatt accggcggag cacaatgcgc taaaacagct
    49501 ggtgaccaaa cttgcggcgg caacggggga acccagcaaa cagatctggc aatcgatgct
    49561 ggaactttcc ggggtgaaag atggcgagtt aattccagcg aaactgttta accatctggt
    49621 gacctggcta caggcgcgtc agacgctaag ccagcaaaat acgccgacgc tggaatcact
    49681 acagatggcg ctaaaacaac ctttagatgc cagtgaactg gcggcgttat cggcatatat
    49741 ccagcaaaaa tatggccttt ctgcgcaatc atcgctttct tctgcccagg ccgaggatat
    49801 tcttaatcag ctttatcaac ggcgggttaa agggattgat ccgcgtgata tgcaaccgct
    49861 gcttaatcct tttccaccga tgatggacac gttgcaaaat atggcaacgc gtcccgcgct
    49921 gtggatactg ttagtcgcga ttatcctgat gctggtctgg ctggttcgtt aaccccgacg
    49981 aaatgacagt atcgtgacaa taatacccag caccgcagag atcgccccgg caagaaatac
    50041 cgaagagtaa ccaaacgtgg tcgccagcat tcccgccagc ggcccggaga cgccgagggc
    50101 gatatcctga aacgcggcgt aaccgcccag tgcggtgccg cgaacttgtg aggggacgcg
    50161 tttaaccacc tccacgccca gcgcaggaaa gataagcgaa catccggctc cggttaacgc
    50221 cgcgcccgct aatgcgaccc atgcacctgg ggcttgccag agcagcaaca agcccaccgt
    50281 ttctacaagc agagagacaa tcgccacttt cacgccgcca aaacggtccg gcatccagcc
    50341 aaacatgacg cgcatcacga caaatgcgcc gccaaacgcg gtaagagtaa agcccgccat
    50401 cgcccatcct ttgctggcaa agtagagcga aacgaaagtc ccgataaccg caaaaccaac
    50461 gccttgtagt gccagcccta accctggttt ccagataagc ccgacaacgc tccacagcga
    50521 tggacgttct cccgccaggg ccggtacttt gcgcactgtg ccgttacagg cccacgccag
    50581 taagggtaat accattgtgg tgatcgccag tgcggcaaaa ccgtaatggc tatgaatcaa
    50641 caggccaagc ggcgcaccaa cagcgagggc accgtaaatc gccattccgt tccatgacat
    50701 cactttgcca gagtgttttg gccctacgat gcctaacccc caggtcagag cgcctgtcag
    50761 taactggctt tcaccaaacc caagaatcaa acgcccgatg accaacaggg caaatttgaa
    50821 cggtgcggag acaggcaaaa tcgccgccag cagcaacgcg ccgccagcca gaccacaagc
    50881 taacatcccc tgaagcgccg aacgttttgc gccatattga tcggccagtc gcccggcgta
    50941 accacgcgtc agcaccgtag ccagaaactg aatcccgacg gcaatgccga ccatggtatt
    51001 gccatagccc agatcatgat gaacaaacag cgggataacc ggcaacggca accctacggt
    51061 catgtaggtg agaaaaaccg caaaagagat gcggaagagc gaaaaactgg cagaagatcg
    51121 tgtttcggtt tggcttacag cagtcatgca ttactccaga atgcagcgca aggcgaggag
    51181 tatccccgtc tcatctctct ggtttcaggg ttacggtgcg ttggcaggat ttaacgcgta
    51241 cgtcttttca gaaggaaatc gacaaagcgg gaagtttgcc tagaactggt ggcgattgtc
    51301 aatgatgtga aaaagggaac catcaggttc ccttttgcgt tagtgccgga ggccgcattg
    51361 gcgcgtaacg tcggatgcga cgctggcgcg tcttatccga cctactccgg cctactgcga
    51421 attaatcttt tcagcttgcg cattaccagc gtggcgttgg tgccgccgaa gccgaagctg
    51481 ttagacataa cagtggtcag ttcgcgatcg gtcgtttcgg tcacgatgtt cagacccgca
    51541 gcctgctcgt ccagctcttc aatgttgatg ctcggggcga taaagccgtg ttccagcatc
    51601 agcagagagt agatagcttc ctgcacaccc gcagcgccca gtgagtgacc ggtcatggct
    51661 ttggttgcag aaatcgccgg gctcttatcg ccgaacactt cacggatagc tgccagctct
    51721 ttcacgtcgc caaccggagt cgaagtaccg tgagagttca ggtaatcgat tggggtatca
    51781 acgccatgca tcgccatctg catacaacgt actgcgcctt cgccagacgg agcaaccatg
    51841 tctgcaccat cagaggttgc gccgtagcca acgatttcag catagatgta agcaccacgc
    51901 gccagcgcgt gttccagctc ttcaaccact accataccgc cgccgccagc gataacgaaa
    51961 ccgtcacggt gagcgtcgta agtacgggag gctttttccg gggtgtcgtt gtatttagta
    52021 gatagcgcac ccatcgcgtc gaattcgcag gccatttccc agcacagctc ttcgccgccg
    52081 ccagcaaaca cgatgtcctg tttgcccagt tggatctgct ctactgcgtt accgatacag
    52141 tgtgcggaag tcgcacacgc ggagctgatg gagtagttaa cgccatgaat tttaaacggc
    52201 gtggcgagac aggcagaaac gccggatgcc atcgctttag tgaccacata cgggccaacc
    52261 gctttcaggc cgcgcgggcc gcgcattgcg tcagcgccga acacctggaa acgcggggaa
    52321 ccgccgccgg aacctgcaat caggccaacg cgcgggttat tctggtaagc ttccggagag
    52381 aggcccgcat cagcgattgc ctgctccata gaaaggaatg cataaatgga tgcgtcgctc
    52441 ataaagcgca caactttgcg gtcaatgagg ccagtggtat ccagttttat gttgccccag
    52501 acgtggctac gcatgccgga atccttcagc tcctgagaga aagtgatccc tgaacgtcct
    52561 tcacgcagag atgccaggac ttcctgctgg ttattaccga tgctggaaac aatgcccagg
    52621 ccagtaatca ctgcacgttt cattcaatac ctctgtaagt cgcacataga gtaagtttcg
    52681 aatgcacaat agcgtacact tgtacgccga acaagtccga tcagccattt aatagagata
    52741 tttgcgcagc cttacacaca tcgctaagat cgagccaccg cctgtaagac gagtaactta
    52801 cgtgaaacac tactccatac aacctgccaa cctcgaattt aatgctgagg gtacacctgt
    52861 ttcccgagat tttgacgatg tctatttttc caacgataac gggctggaag agacgcgtta
    52921 tgtttttctg ggaggtaacc aattagaggt acgctttcct gagcatccac atcctctgtt
    52981 tgtgatagca gagagcggct tcggcaccgg attaaacttc ctgacgctat ggaaggcatt
    53041 tgatcagttt cgcgaagcgc atccgcaagc gcaattacaa cgcttacatt tcattagttt
    53101 tgagaaattt cccctcaccc gtgcggattt agccttagcg catcaacact ggccggaact
    53161 ggctccgtgg gcagaacaac ttcaggcgca gtggccaatg cccttgcccg gttgccatcg
    53221 tttattgctc gatgaaagcc gcgtgacgct ggatttatgg tttggcgata ttaacgaact
    53281 gaccagccaa ctggacgatt cgctaaatca aaaagtagat gcctggtttc tggacggctt
    53341 tgcgccagct aaaaacccgg atatgtggac gcaaaatctg tttaacgcca tggcaaggtt
    53401 ggcgcgtcca ggcggcacgc tggcgacatt tacgtctgcc ggttttgtcc gccgcggttt
    53461 gcaggaagcc ggattcacga tgcaaaaacg taagggcttt gggcgcaaac gggaaatgct
    53521 ttgtggggtg atggaacaga cattaccact cccctgctcc acgccgtggt ttaatcgtac
    53581 gggcagcagc aaacgggaag tagcgattat cggcggtggt attgccagcg cgttgttgtc
    53641 gctggcgtta ttacggcgcg gctggcaggt agcgctttat tgcgcggatg aagcccccgc
    53701 gctgggtgct tccggcaatc gccagggggc gctgtatccg ttattaagca aacacgatga
    53761 ggcgctaaac cgctttttct ctaatgcgtt tacttttgct cgtcggtttt acgaccaatt
    53821 acccgttaaa tttgatcatg actggtgcgg cgtcacgcag ttaggctggg atgagaaaag
    53881 ccagcataaa atcgcacaga tgttgtcaat ggatttaccc gcagaactgg ctgtagccgt
    53941 tgaggcaaat gcggttgaac aaattacggg cgttgcgaca aattgcagcg gcattactta
    54001 tccgcaaggt ggttggctgt gcccagcaga actgacccgt aatgtgctgg aactggcgca
    54061 acagcagggt ttgcagattt attatcaata tcagttacag aatttatccc gtaaggatga
    54121 ctgttggttg ttgaattttg caggagatca gcaagcaaca cacagcgtag tggtactggc
    54181 gaacgggcat caaatcagcc gtttcagcca aacgtcgact ctcccggtgt attcggttgc
    54241 cgggcaggtc agccatattc cgacaacgcc ggaactggca gagctgaagc aggtgctgtg
    54301 ctatgacggt tatctcacgc cacaaaatcc ggcgaatcaa catcattgta ttggtgccag
    54361 ttatcatcgc ggcagcgaag atacggcgta cagtgaggac gatcagcagc agaatcgcca
    54421 gcggttgatt gattgtttcc cgcaggcaca gtgggcaaaa gaggttgatg tcagtgataa
    54481 agaggcgcgc tgcggtgtgc gttgtgccac ccgcgatcat ctgccaatgg taggcaatgt
    54541 tcccgattat gaggcaacac tcgtggaata tgcgtcgttg gcggagcaaa aagatgaggc
    54601 ggtaagcgcg ccggtttttg acgatctctt tatgtttgcg gctttaggtt ctcgcggttt
    54661 gtgttctgcc ccgctgtgtg ccgagattct ggcggcgcag atgagcgacg aaccgattcc
    54721 gatggatgcc agtacgctgg cggcgttaaa cccgaatcgg ttatgggtgc ggaaattgtt
    54781 gaagggtaaa gcggttaagg cggggtaatc tgctctcgca gtgtttgccg gatgcggcgt
    54841 gaacgcctta tccaacctac gtgtgacgtg taggcctgat aagacgcgat aagcgtcgca
    54901 tcaggcatgg tgctccagac gccgcaactt acagctgtga cgcctgctga aacaggtttt
    54961 cccacatatc ggtcaccagc gcctggtcac gcggcgacaa ttcaccggca ccaatggctt
    55021 tttccagact ctggctaacg gtcgtatgca ccgcctgagc ggagtggtca tcaccacttt
    55081 ccagttctgc gatggctaat gtcaggtggc cacgcaaata cccactggca aacaactcat
    55141 catcactggc atggtcaacc atgccgtcga ttaatgccag aatgcgtgat tcaaactccg
    55201 cgatcatctt ctttcctcat tgtaaaacgt ggcgctggct tcagttgagc gcttccggcc
    55261 acgggaactg ttccgccgta agttccggcg tgtgataata attttgtagt gctttaatga
    55321 agcgtgccgg gcgttccgga atgccatttg tcagataatc catcacctgc gcatgtaccc
    55381 gccgttgaaa caccacacga tccggttcga aatccccttc cagattgtcg caactgacgt
    55441 taaacggata tccagccgcc acgcagaata accagtcgaa cgcctgcggc ttcacttcaa
    55501 catcttcaaa ctggctttgc gtctgggcat cgcgtccgtc cgggcaatac cagtagccga
    55561 aatcaaccag ctcacggcgc gcttttccgg caatacacca gtgcgaaatc tcatgaattg
    55621 cgctggcata aaagccatga gcaaagacga ttcggttata cggtacttcc gcatcagcag
    55681 gaagatagat cggttcgtcg tcgcctttaa tcagacgggt attaaattca tcggcaaagc
    55741 agctattaaa aatttcaatc aactgctcgt agtggtgtgt actgttcatt agttcatccc
    55801 caaccagtgg aggatctcct gtccgtggct atcgtaaagt aatttggcac tcatcaccgc
    55861 cgagacgata acaatcatcg ggcggatcag cttttgtcct ttgctcaaca ccaaccgtga
    55921 acccatgcgc gcgccgagga actgccccac cagcatgaca aagcccgtcg cccaaatcac
    55981 tttgccgccg agaataaaca gcagcagacc gccaatgttt gacgttgcgt tgagtaattt
    56041 ggcgtgagcc gtggctttgg cgaggttgaa tccgcacagc ataacgaagg ccagcgcgta
    56101 aaacgatccg gcagccgggc caaagaatcc gtcataaaaa ccgacgcagc caccggcaat
    56161 taacgcgaag ggtaaaccgt acatccggcg ctggcgatct tcttcaccca gctttggcat
    56221 cagcaagaaa tagaggccga tacaaatcac cagaatgggc aaaatctgcc gtaagacatc
    56281 agcctgaacg tactgtacca acagtgcgcc gctcattgag ccgacaaagg tcatggcaat
    56341 attgagtttc tgatcgctta aactaaccac tttgcggcga ataaagtaga tagtggcgga
    56401 aatagagccg ccgcaggctt gcagtttatt ggttgccagc gcgttagcgg gagacattcc
    56461 cgcagccatc aatgccggaa tggtgagtaa cccaccgcca ccggcaatcg agtcgataaa
    56521 tcccgccagc atggcgacaa aaaagagaac tcccagcaac agcggagaaa ccataaacag
    56581 gctattaaac gtttccatta gatcacgtgc tcatccagta gcgcctggca ggaaggcggc
    56641 aacggaggcg gtgtcttctt ctcaggcttt gttgttcccg gttttggagg ttcaaaccag
    56701 ctttgcagtt ctgccccgca accatcgcct gatggcggta aaggttgatc ttcacactcc
    56761 agactatcgg cagggcaacg taatcgtaca tgcatatgcg cgcgatgctg gaaccagggt
    56821 cgcactttgc gcaaccagtc gcgatcggtg cccgcatcaa ggcaaagttg ttgtttaatc
    56881 gccggattaa caaaaatgcg cgtgacgtct ttgtcctggg cggcgagttt gatcaagctg
    56941 aaaatttctg gcttccacag cgtggagaca acgtgtttac cgtcgcggga aactaagtcc
    57001 agtgcttgcg ggcgcaagag ctgcgcggag gtccagcgag ttttcggcag ttgcagaaag
    57061 atatcgacat ccagtccggt ctggtggctg gcatgaccgc cgttgaaacg cccaccagcg
    57121 ggcatcccca tatcgccaat cagcaccgta cccatgccca gattgctcac ctggctactc
    57181 agacgctgga taaacatcac cagatccggg tgaccgaaat agcgacgctg atcggtacgc
    57241 atgacctgat aatgttcgga ctgtatcggc aacgtgtcag cgccgacaat acagccatta
    57301 gaaaaactgc ctatcgattg tgcgctaccc ggcacaggtt gggttatttt ttgccacggc
    57361 gtcgctgcca ggctagcact actggcaagc agagccagca gcgcaatcgc ggttttgttc
    57421 attttttacc agcgtggaat atcagtcttc acatcggcat tttgcgcccg ctgccgtaac
    57481 aggtgatcca ttaaaacgat cgccagcatc gcttcagcga tcggcactgc acggatcccg
    57541 acacagggat cgtgacggcc tttggtgatc atctcaactt cttcgccaaa gcggttaata
    57601 gtgcgccccg gcacggtaat gctggaggtc ggtttcagcg ccatatgagc aatgatttgc
    57661 tgcccgctgc tgataccgcc gaaaatgccg cccgcatggt tgctctggaa accgtctttg
    57721 gtgatttcgt cgcggttctg gctgccgcgc agcgccacta cgtcaaaacc gtcgccaatt
    57781 tccacgcctt tcaccgcgtt gatgctcatc agcgcatggg cgatgtcggc atccaggcgg
    57841 tcaaagaccg gctcgccaag tccggcgggg acaccactgg caacaacggt gactttcgcg
    57901 ccgatggagt cgccctcttt tttcagcgcg cgcatcagtt catctaacgc gtcgattttg
    57961 tcggggtccg ggcagaagaa cggattttgt tcaaccagcg accagtcttt gatttccagc
    58021 ggaatgtcgc ccatctgggt caggcagccg cggatttcaa taccaaattt ctcggcgaga
    58081 tattttttgg caatcgcccc tgccgccacg cgcatggcgg tttcgcgggc ggaagaacgt
    58141 ccaccgccgc gataatcgcg cagaccgtat ttttgttcgt aggtgtaatc agcatggcct
    58201 ggacggaaga cgtctttaat tgcgctgtaa tcctgagaac gctggtcggt attttcgatc
    58261 aacaagccaa tgctggtgcc agtagtaacg ccttcaaaaa caccggagag tattttgacc
    58321 tgatccggct cgcggcgctg ggtggtatag cgcgatgtcc cagggcgacg acgatcaagg
    58381 tcatgttgca ggtccgcttc cgtcagcgga atgcctggcg gaacaccatc gacgatgcag
    58441 ccgagcgcca gcccgtgcga ttcgccgaag gtggttacgc gaaagagttg tccaattgtg
    58501 tttccagcca tcacggctcc gttattgttg tgtttgcgtg tttacttaat ctttataaat
    58561 cgcgaaatgt tctcgtgcgg caataagctg ctctttagtg agcataaaca caccatcgcc
    58621 gccgttatca aactccagcc aggtgaacgg aacatcggga tattgttcca taagatgtac
    58681 catgctgttg cccacttcac aaatcaacac gccatcatca gcaaggtaat ctgccgcgtt
    58741 accgagaatg cgacgcgtca gtttcaggcc gtcggtgcca gatgccaggc ccagttccgg
    58801 ctcgtgacgg tattcgtttg gcaggtcgga catatcttcc gcgtcgacat acggcgggtt
    58861 agtgacaatc agatcgtatt gcactttcgg caaatcacgg aacagatcgg aacgaatcgg
    58921 aatgacgttg tggatcagac cgtgttcttc gatgttctgt tcggcaaccg ccagcgcgtc
    58981 gggagagata tctaccgcgt cgacttccgc ttccgggaag gcataagcac aggcaatggc
    59041 gatgcagccg ctaccggtac acatatctaa aatatgctgc ggttgcttgc tgatgagtcc
    59101 ggcaaattta ttgttgatca gttcaccaat cggcgagcgc ggcaccagca cgcgttcatc
    59161 gacgtaaaat tcatgaccgc agaaccaggc tttgttggtc aggtaagcca ccggaatgcg
    59221 ctcgttgacg cggcggatca cgcgttcaac aatacggtgt ttttcgctgg aggtcagacg
    59281 cgcggtgcgc atatcttccg ggatatccag cggcaggtag agcgatggta acaccagctg
    59341 tacggcttca tcccacgggt tatcggtgcc gtgaccgtac cagatatttg ccgcgctgaa
    59401 gcggcttacc gaccagcgca atatgtcctg aatggtttgc agctcattta ctgcttcatc
    59461 aacgaaaatt ttatccacgt attcctccag ggcatgatcg caataatttc ggcggctagt
    59521 ttgccatgaa gatgacgata aatcagcatt cacgcgcggt gagtgaggaa aaatacgttt
    59581 aaaacgatcg attgtgctac gagtcgggta aactgtagga aaattagaaa tagagacaga
    59641 taaatgaaaa agaaaacaac acttagcgag gaggaccagg ctctgtttcg ccagttgatg
    59701 gcggggactc gcaagattaa gcaggacacg attgtccacc gaccgcagcg taaaaaaatc
    59761 agcgaagtgc cggtgaaacg cttgatccag gagcaggctg atgccagcca ttatttctcc
    59821 gatgagtttc agccgttatt aaataccgaa ggtccggtga aatatgttcg cccggatgtc
    59881 agccattttg aggcgaagaa actgcgccgc ggcgattatt caccggaatt gtttttggat
    59941 ttacacggtc tgacgcagct gcaggccaag caggaactgg gcgcgttgat tgccgcctgt
    60001 cgccgcgaac atgtgttttg tgcctgcgtg atgcatgggc acgggaagca tattttgaag
    60061 caacaaacac cgctgtggct ggcacaacat ccgcatgtga tggcatttca ccaggcaccg
    60121 aaagagtacg gcggtgatgc ggctttgttg gtgttgattg aagtggaaga gtggttgccg
    60181 ccggagttgc cctgaatgct ggcagcaaat gccggagcag cgtaaacgcc ttatccggcc
    60241 tacaaatatg tgataattca ataaattgca attcacttcg taggcctgat aagacgcatc
    60301 agagcaatac tacgaacttc agttcattca atacatactg aaaacgacac gtcgaatcaa
    60361 tcggttgccc actactggta ttcacattca ccacaatatc gtgtagtact ttatccgtta
    60421 ccggatgacg agttggcgta accgttaccg ttgatttatc acaaccgtaa gaggtaaagg
    60481 ttaacaattc tgataccgcg cggcagtatg ctcctctgcc atcattatca gaaaaattac
    60541 tgccatcaca gagtctgccg ccttccatca ggtaggtcga aagcgtggtg gtcactgttg
    60601 accccgtgga ctgaattcgt aacgccttat tatggctgac ataattttca ctccaggaga
    60661 tatcgtatga agcgaccggt atcgttgggc atattgtgtt atgcactgta ggggccgtat
    60721 agcttccaaa tccgggtaaa tctgtatagg aataaaagga tgcgatccaa gggcaaccat
    60781 aatcatcaga tgcaacggta attttaaacg tcatatatgc acaacgaaaa attttcgtat
    60841 ctatgccagg gcaatttgag ttattaattg tatcaaagtc agtcagtacc aaagttggag
    60901 ctgaacccga cccagaccag gaaataaccc gcaacgtatc gcctttatac gcaaaagaat
    60961 catatttccc atatgcaacc tcctttggcg ttatcaccgt aacattgaaa acactgctcg
    61021 ctacagcacc ggttaccttg tccatggcat aataaactct cacactgtca aaactcgcac
    61081 tcataagttt atctccagca taactcttag atgcgccgac aacactcaga acgagcagca
    61141 ttaggctcgc gaaaattatg gtccattttt tcatttcaga aatactcaaa ggttgccgtc
    61201 actgtggcac taaagtctcc ggacgttaca tctcgcccgc tctttgcctg taaccaggta
    61261 ttaaaattta ggctgttatt tccctgattc agcgggagcg tgacgcctgt tgtattgttg
    61321 attggcaccg atgtgcctgc cgcagtttca ataccaatcc cagcgccgga tgctgatgac
    61381 gaggagtcaa aggccagaaa gccgggtaga gcactatctt ctgttcctgt caacgttact
    61441 ttgacattaa acaatgtaga actatgacaa tctttcaatt gaaacaccaa tggcactcgc
    61501 tctgactgcc caaggttcat taaatcactg gcggcaatgg caggaaaatg tacttctgcc
    61561 aactggctac cattaatcac tggcgtacac gatttactga ttaaattgcc ggtaaagtgc
    61621 aaattatctc ctgctattgt ggttccactg cccagcaaca tcaggctact aataaaaaac
    61681 aacgtacgct ttttcttcat tggtaatcta cctgtaacgt ggcccaggcc tcaaagtccg
    61741 cctctggcag aatgacacca ctctttttta ctggcacagc cgttaacacc ggtaaatcgg
    61801 tttcattcac cacaagtggc gtattgatcg taaaactctg ccctgcctgt ttgagctgaa
    61861 tgcctaaacc agtgatattg ctgcttactg cagcattatc aaaatcgctt ggtgtgccac
    61921 tccagctcaa ggtcatttcc attaatgggt cgcgaaccgc cgtatcgcag gtaatggaat
    61981 acggcacggt ttgctcataa ttgctgccat taatgtcatc aataagcaca tcctgaaaag
    62041 ttatttcaat ggtttgagcg ttgctaatcg tgcagttagg cggctgcact aaataaccgt
    62101 gaaaagagac gtttgagagt gccatcgagc aaaagcccca cagcaaaata agcgagatcc
    62161 gtttcattgg taatccacca caattgtggc actggcgtta aattcagctg ccgctaacgt
    62221 tgtaccactc tgtttcaccg gcacagcttc cagaacggga actcccgagg aaaaattaaa
    62281 tggcaaccat gagccagagg ttaagtcgag tattgtgtga tcactacttt tctgaacccg
    62341 aataccaaac ccaggaatac ccgtgctgat aactgtttca ccgttaatca cgaccgttgt
    62401 cgcctgcatc tgtagacgca ggtcatccat tgccagattg ttacaaacaa gcgagtagtc
    62461 aatgggacgt ttataatcaa ccctattgat cttattaatg aatacattac caaactcaac
    62521 tgccgcacca tttaccgtac aaggaggagg ggcatcgaca agcacccgta aattcatact
    62581 tactgactta ctcgctgcaa ctgcaaagcc gctaccaaca caacatatca gtaacccagt
    62641 taccgcccag acagaacaag atcctttcat actggctctc ccggcctcag cctttgcggc
    62701 tctgctcttc atcgacctag caactgttac cgttgcaagt gaaaaagagc ggcatccgag
    62761 cgccatagtc gttgacatag gtcagtaccg gcacgctacc catcttcaca ttcagcggtg
    62821 cagaggtttt cggcgacatc actattggcg agaaaccggc tgccggattg ccatttttct
    62881 gtgtgctggc attactgaaa atgacgtaat acgacgtcgg gttattgacg gtaaattcct
    62941 gaccgttgcg ggttaaagta atcttatatt gctaggggtt tttcatgctg acgttctcca
    63001 gcgccttcgg tcgccagaac aacttaatac gcgtttgtag tgctatttgc agcgtgttgg
    63061 ctttgtttga ttttggcggg atctcacgca cgttgaagta gaacaggctt tcacgatcgg
    63121 caggcaattt gttgatatcc ggcattccct gcacttttac ctgcccgttc atcattgaat
    63181 cgatgcgttg caccggtggc agaacagtta acggtgaact gatcttgtta cccttttcat
    63241 cttcgatcca gctttgggcc agataaggca gtttcggatc gttattgcgc aacgtcacgc
    63301 tgattgattt gtcgctttca ttaaatatca aacgggtacg atctggcgtt acagaagcct
    63361 gggcagacaa actggcagcg ctaagcagta acgctagcgt cgtagcaccc aatttggcag
    63421 agcaaagtaa atcagacatg gatcattcct taatcacgtt attggttagc tgaaacagaa
    63481 acagggggat tggtgggtgt cacctgttgc gtctgctcct gaatgacagg ctttatgtca
    63541 tcaggaacaa ccggcgcgac gttgccttta tgctggcatg gcagtaacaa gccgttaaac
    63601 agatcggcag gcagcgggtc cggcaggttg atatcgcaat gcgcaacacc actccagaac
    63661 acactcatgt gttcgccagg tttcactcca gccagataaa cattgccgtc atcatcgaca
    63721 aggcccactg tctgctcgtt atcatttttt acttctgcgc caaacggagg atggctgccg
    63781 tcttgtaaac gcagcaccgc catcgctttt tgaccactga tgacggcaaa tttgcggtag
    63841 ccaatcgccc cttcagttag cgtggcttgt accaccgact gggttgcttc ggcgttttcc
    63901 ggcagtttgt tgaggtcgat atacgcctga ttgcggtaat agttattgac gtcagaaacg
    63961 acggctttac caaacatatt ggtataaaca gcagcaccgt taccttctac cggaacatcg
    64021 gcaacgccat cggcatcaat caacaagcgt gtcccgccca tattctgggt acggtgaagt
    64081 gcgccaccgt gggcagtaag cgttgcgccg ccctgtaacg acaagcccgc agaggtgtat
    64141 tgcccttcat ggtagttcgc actgaggtca acctgcgcca gcgaaccatc atggctgtaa
    64201 tagccgtcaa cgctggtgtg tttgtcactg gtgccgacgt tcaactgata gtgagtcgcg
    64261 tcatcgacac ggctgaaata accgacctga ctgctgtccg tcccactgcc atagtaccgt
    64321 tatagctaac ggtactgttg tcgccccacg gcatactgag cgaaatgtac atgcctttgt
    64381 cggcctggtt gtcatactcg taacggtagc cagtcatcga gatgctgaca ttacgaatac
    64441 tgcccatatt gaaatagtgc gagagcatga tgttgtagtt tgtctgctcc tcgcgatccc
    64501 agtaggtatg gcgggtatag ttgagataaa ccgaaacacc cgcatcgcgg aagttctggt
    64561 tataagtggc ggtgtacatc tctttgtcgt tgcccgtgcg taccattccg ctgtcactgg
    64621 catccagata ctcgctcatg gtcataaagt tctcttccga gaagcgatat ccagcaaaag
    64681 taacgcggct gttgagctgg tcaaagtctt tggaataact cacacggaag gagttaccgt
    64741 ccagcgaacc tttgccataa gcggtgtctt tatccagttt ggtgtgcgag tgagtaacat
    64801 caaacgcgac cgcgccgaat gtagacaaat cgcgaccgac accaagcgcc gcagactgat
    64861 agttttcatc tcccagtgcg ccgccatata acgaccagcc gttagcgatc ccccaggaag
    64921 cttcagcacc agaaaaaaat tcaccctcga catggtgtcc ccactcttgc ggacggccca
    64981 tcatgatctt atagcgaacc tgacctggac gagtgaggta tggcatcgag gcggtgctga
    65041 tgtcatattc ctgcacctgg ccgttctgtt cttcaatgcg aatatgcaac gtaccggaga
    65101 cagaatcacc aagatcctga atacgaaacg gtccagccgg aacctgcgtt tcgtaaatca
    65161 cacgccccat ctggctgacg gtcacttttg ctgtggtgtg tgccacgccg gaaatgtctg
    65221 gcgcgtagcc gcgcagattg ggaggcaaca tttgatcgtc agtactgaca ctgccaccaa
    65281 catagttaaa accatcaaaa atatcggatc tgaggtaatc ctcgcccagc gccagtttgg
    65341 cttttaatga tggtaacgcc cgcctggcat agtagcgact ccactcccat ttttttgagt
    65401 ttcatcgccg ctgaattcat cgtcatcatt actgcgagta tgttgatagt tagtctgcca
    65461 gtcagcacgc atacgccacg gccctaggtt aaccccaacc gtcccgttgc cgctgatctc
    65521 gttactatca tcaccgccat tttcttcgtg ccgggtttgt gcgttgatgc tgtagtccgc
    65581 gacgatcccg gagatgccgt catcccaacg tgaaggcgga tccctgtcgg gataagtata
    65641 ttcgaggtaa gcctgtggca gtgaaatgac taatgcggac tggcttaaat cagccttaat
    65701 ttccatgcct tccagttgac cggatttcag gcatttagca tcgtggctcc attgcagatt
    65761 tttcgccacg tcttctttta aaccaaactg cgctaccagt tccggtgtca gacaagcata
    65821 gcttttgctc gcgtcatctt caccagcata ccagtaaata tcgtactctt ccgccaatgg
    65881 ctgtttattt agttgaacct gtaaattgta ttttccgggc tcaacatatc cctggctgga
    65941 aaaacgcttc atatcaattt ttgtgtcgcc ttttaattcc agaaaacggg aatcaaactg
    66001 aatgtcgtct tcagcccaga cactgctata actccctgac attgccagcg caatgcacca
    66061 cggaagaatc cgtaatcgaa aaagagaatg gtcaggcata cacgtttcta tccgttttta
    66121 ttcttgttcg gtcaaaatac atttaaatcc cccatagcag tgtcgctatg ggggtatgcc
    66181 ttacaggtaa gtaatctgga aggtggtatt tgcttcaaaa ttacccagat ctggcgcatc
    66241 agctgcgccg accagccagg cgttaaagtt cagtgtctgc ttcgctttgc ctttgttggt
    66301 agaagtgtct ttcactattt tctggtcaat acccatgccg cttttgtaag aggtacccag
    66361 agagtcaccg atcgccaggc taacattgtt aaatgccgca ccggtatcgg tattgaaaat
    66421 ggtgtaataa ttgccgctat ttgcagaaga aacggtacca gtaaaggtag tggtcattgt
    66481 ttcctgagta tcaaatacgc aatcttgcag acgaatctgg aaacttttcg gcgtggtggt
    66541 gccgttattt ttcagttttt cggcaccgat atcacccata tcaacttcca gtttgtgatc
    66601 gtccggaacg atagaacaca ctgagtcttc aatggtgccg taaaaacgtg cagtaccgtt
    66661 gttacccgca gcgattgttg ccgtagaggt gaacacgccc atcaccattg ccgcagcaat
    66721 agctgtttta acaaacttac tcattatttg tccttaaact ataaataatt aaaaatataa
    66781 acattgcaat acattgattc agtcaatagc caatgtttat acccagccat tgaatacggc
    66841 atgcgaccca aaaagaacag gtcgtagaaa attgtattca ttggtgtggg gccgcatagt
    66901 gatgtttcca cacccctgat ccaatttaat tttactaaag agtaaatgta gatttaatta
    66961 atatattgac agaagaaagg ttatcttaag ga
//
LOCUS       DAEBXN010000005        66032 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.005, whole genome shotgun sequence.
ACCESSION   DAEBXN010000005 DAEBXN010000000
VERSION     DAEBXN010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 66032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66032
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.005"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(128..607)
                     /gene="folK"
                     /locus_tag="KJL99_000311"
     CDS             complement(128..607)
                     /gene="folK"
                     /locus_tag="KJL99_000311"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HBD4435191.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSPAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            complement(604..2022)
                     /gene="pcnB"
                     /locus_tag="KJL99_000312"
     CDS             complement(604..2022)
                     /gene="pcnB"
                     /locus_tag="KJL99_000312"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HBD4435192.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTT"
     gene            complement(2061..2987)
                     /gene="gluQRS"
                     /locus_tag="KJL99_000313"
     CDS             complement(2061..2987)
                     /gene="gluQRS"
                     /locus_tag="KJL99_000313"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HBD4435193.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDLLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQIPLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWMLTAVPESAIVNSTFSNASC"
     gene            complement(3024..3479)
                     /gene="dksA"
                     /locus_tag="KJL99_000314"
     CDS             complement(3024..3479)
                     /gene="dksA"
                     /locus_tag="KJL99_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HBD4435194.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3657..4361)
                     /gene="sfsA"
                     /locus_tag="KJL99_000315"
     CDS             complement(3657..4361)
                     /gene="sfsA"
                     /locus_tag="KJL99_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HBD4435195.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGSERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4376..4906)
                     /gene="thpR"
                     /locus_tag="KJL99_000316"
     CDS             complement(4376..4906)
                     /gene="thpR"
                     /locus_tag="KJL99_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HBD4435196.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4980..7409
                     /gene="hrpB"
                     /locus_tag="KJL99_000317"
     CDS             4980..7409
                     /gene="hrpB"
                     /locus_tag="KJL99_000317"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HBD4435197.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGLNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMVRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVVLCPLYGVLSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVNAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANSMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLKLLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7605..10139
                     /gene="mrcB"
                     /locus_tag="KJL99_000318"
     CDS             7605..10139
                     /gene="mrcB"
                     /locus_tag="KJL99_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HBD4435198.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10359..12602
                     /gene="fhuA"
                     /locus_tag="KJL99_000319"
     CDS             10359..12602
                     /gene="fhuA"
                     /locus_tag="KJL99_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HBD4435199.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLFNLYNPVNPDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTNKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12653..13450
                     /gene="fhuC"
                     /locus_tag="KJL99_000320"
     CDS             12653..13450
                     /gene="fhuC"
                     /locus_tag="KJL99_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HBD4435200.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13450..14340
                     /gene="fhuD"
                     /locus_tag="KJL99_000321"
     CDS             13450..14340
                     /gene="fhuD"
                     /locus_tag="KJL99_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HBD4435201.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKHPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14337..16319
                     /gene="fhuB"
                     /locus_tag="KJL99_000322"
     CDS             14337..16319
                     /gene="fhuB"
                     /locus_tag="KJL99_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HBD4435202.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPSAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLIFAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16354..17634)
                     /gene="hemL"
                     /locus_tag="KJL99_000323"
     CDS             complement(16354..17634)
                     /gene="hemL"
                     /locus_tag="KJL99_000323"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HBD4435203.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYFAVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGIHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17859..19280
                     /gene="clcA"
                     /locus_tag="KJL99_000324"
     CDS             17859..19280
                     /gene="clcA"
                     /locus_tag="KJL99_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HBD4435204.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMGLGREGPTVQIGGNISR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19304..19369
                     /locus_tag="KJL99_000325"
     CDS             19304..19369
                     /locus_tag="KJL99_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4435205.1"
                     /translation="MAIIIGLEFARLPMSFGAKYE"
     gene            19362..19706
                     /gene="erpA"
                     /locus_tag="KJL99_000326"
     CDS             19362..19706
                     /gene="erpA"
                     /locus_tag="KJL99_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HBD4435206.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19753..20376)
                     /locus_tag="KJL99_000327"
     CDS             complement(19753..20376)
                     /locus_tag="KJL99_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HBD4435207.1"
                     /translation="MLVYWLDIVGTAVFAIYGVLLAGKLRMDPFGVLVLGVVTAVGDG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GISVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLVREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20414..21214)
                     /gene="btuF"
                     /locus_tag="KJL99_000328"
     CDS             complement(20414..21214)
                     /gene="btuF"
                     /locus_tag="KJL99_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HBD4435208.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYVQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNLVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21207..21905)
                     /gene="mtnN"
                     /locus_tag="KJL99_000329"
     CDS             complement(21207..21905)
                     /gene="mtnN"
                     /locus_tag="KJL99_000329"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HBD4435209.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21989..23506
                     /gene="dgt"
                     /locus_tag="KJL99_000330"
     CDS             21989..23506
                     /gene="dgt"
                     /locus_tag="KJL99_000330"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HBD4435210.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23636..25060
                     /gene="degP"
                     /locus_tag="KJL99_000331"
     CDS             23636..25060
                     /gene="degP"
                     /locus_tag="KJL99_000331"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HBD4435211.1"
                     /translation="MKKNTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKKLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25215..26371
                     /gene="cdaR"
                     /locus_tag="KJL99_000332"
                     /pseudo
     CDS             25215..26371
                     /gene="cdaR"
                     /locus_tag="KJL99_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
     gene            complement(26460..26846)
                     /locus_tag="KJL99_000333"
     CDS             complement(26460..26846)
                     /locus_tag="KJL99_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HBD4435212.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27160..27984)
                     /gene="dapD"
                     /locus_tag="KJL99_000334"
     CDS             complement(27160..27984)
                     /gene="dapD"
                     /locus_tag="KJL99_000334"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HBD4435213.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQATPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28015..30687)
                     /gene="glnD"
                     /locus_tag="KJL99_000335"
     CDS             complement(28015..30687)
                     /gene="glnD"
                     /locus_tag="KJL99_000335"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HBD4435214.1"
                     /translation="MNTLPEQYANTALSTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30749..31543)
                     /gene="map"
                     /locus_tag="KJL99_000336"
     CDS             complement(30749..31543)
                     /gene="map"
                     /locus_tag="KJL99_000336"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HBD4435215.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31911..32636
                     /gene="rpsB"
                     /locus_tag="KJL99_000337"
     CDS             31911..32636
                     /gene="rpsB"
                     /locus_tag="KJL99_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HBD4435216.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIVSRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32894..33745
                     /gene="tsf"
                     /locus_tag="KJL99_000338"
     CDS             32894..33745
                     /gene="tsf"
                     /locus_tag="KJL99_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HBD4435217.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33892..34617
                     /gene="pyrH"
                     /locus_tag="KJL99_000339"
     CDS             33892..34617
                     /gene="pyrH"
                     /locus_tag="KJL99_000339"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HBD4435218.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34909..35466
                     /gene="frr"
                     /locus_tag="KJL99_000340"
     CDS             34909..35466
                     /gene="frr"
                     /locus_tag="KJL99_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HBD4435219.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35558..36754
                     /gene="ispC"
                     /locus_tag="KJL99_000341"
     CDS             35558..36754
                     /gene="ispC"
                     /locus_tag="KJL99_000341"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HBD4435220.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEEVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANASEVARKE
                     VMRLAS"
     gene            36943..37701
                     /gene="ispU"
                     /locus_tag="KJL99_000342"
                     /gene_synonym="uppS"
     CDS             36943..37701
                     /gene="ispU"
                     /locus_tag="KJL99_000342"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HBD4435221.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37714..38571
                     /gene="cdsA"
                     /locus_tag="KJL99_000343"
     CDS             37714..38571
                     /gene="cdsA"
                     /locus_tag="KJL99_000343"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBD4435222.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38583..39935
                     /gene="rseP"
                     /locus_tag="KJL99_000344"
     CDS             38583..39935
                     /gene="rseP"
                     /locus_tag="KJL99_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HBD4435223.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFDSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39965..42397
                     /gene="bamA"
                     /locus_tag="KJL99_000345"
     CDS             39965..42397
                     /gene="bamA"
                     /locus_tag="KJL99_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HBD4435224.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42519..43004
                     /gene="skp"
                     /locus_tag="KJL99_000346"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42519..43004
                     /gene="skp"
                     /locus_tag="KJL99_000346"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HBD4435225.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43008..44033
                     /gene="lpxD"
                     /locus_tag="KJL99_000347"
     CDS             43008..44033
                     /gene="lpxD"
                     /locus_tag="KJL99_000347"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HBD4435226.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44138..44593
                     /gene="fabZ"
                     /locus_tag="KJL99_000348"
     CDS             44138..44593
                     /gene="fabZ"
                     /locus_tag="KJL99_000348"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBD4435227.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44597..45385
                     /gene="lpxA"
                     /locus_tag="KJL99_000349"
     CDS             44597..45385
                     /gene="lpxA"
                     /locus_tag="KJL99_000349"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HBD4435228.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45385..46533
                     /gene="lpxB"
                     /locus_tag="KJL99_000350"
     CDS             45385..46533
                     /gene="lpxB"
                     /locus_tag="KJL99_000350"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HBD4435229.1"
                     /translation="MTDQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46530..47126
                     /gene="rnhB"
                     /locus_tag="KJL99_000351"
     CDS             46530..47126
                     /gene="rnhB"
                     /locus_tag="KJL99_000351"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HBD4435230.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            47163..50645
                     /gene="dnaE"
                     /locus_tag="KJL99_000352"
     CDS             47163..50645
                     /gene="dnaE"
                     /locus_tag="KJL99_000352"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HBD4435231.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLALLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKSVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50658..51617
                     /gene="accA"
                     /locus_tag="KJL99_000353"
     CDS             50658..51617
                     /gene="accA"
                     /locus_tag="KJL99_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HBD4435232.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51716..53857
                     /gene="ldcC"
                     /locus_tag="KJL99_000354"
     CDS             51716..53857
                     /gene="ldcC"
                     /locus_tag="KJL99_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HBD4435233.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GISTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVFFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVKTAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECRPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIAPEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53914..54303
                     /locus_tag="KJL99_000355"
     CDS             53914..54303
                     /locus_tag="KJL99_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBD4435234.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54368..55666
                     /gene="tilS"
                     /locus_tag="KJL99_000356"
     CDS             54368..55666
                     /gene="tilS"
                     /locus_tag="KJL99_000356"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HBD4435235.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSATLCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVLWQTWLQPLELPA
                     GLGTVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(55715..55975)
                     /gene="rof"
                     /locus_tag="KJL99_000357"
     CDS             complement(55715..55975)
                     /gene="rof"
                     /locus_tag="KJL99_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HBD4435236.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55962..56162)
                     /locus_tag="KJL99_000358"
     CDS             complement(55962..56162)
                     /locus_tag="KJL99_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HBD4435237.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56328..56873
                     /locus_tag="KJL99_000359"
     CDS             56328..56873
                     /locus_tag="KJL99_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HBD4435238.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            56870..57292
                     /gene="arfB"
                     /locus_tag="KJL99_000360"
     CDS             56870..57292
                     /gene="arfB"
                     /locus_tag="KJL99_000360"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBD4435239.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTKEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57306..58016
                     /gene="nlpE"
                     /locus_tag="KJL99_000361"
                     /gene_synonym="cutF"
     CDS             57306..58016
                     /gene="nlpE"
                     /locus_tag="KJL99_000361"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HBD4435240.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIEISLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(58171..58995)
                     /locus_tag="KJL99_000362"
     CDS             complement(58171..58995)
                     /locus_tag="KJL99_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HBD4435241.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSDLAKAQLSSVCEGDKKSWFCSEFVTDAFAKS
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(59048..60766)
                     /gene="proS"
                     /locus_tag="KJL99_000363"
     CDS             complement(59048..60766)
                     /gene="proS"
                     /locus_tag="KJL99_000363"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBD4435242.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60877..61584)
                     /gene="tsaA"
                     /locus_tag="KJL99_000364"
     CDS             complement(60877..61584)
                     /gene="tsaA"
                     /locus_tag="KJL99_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HBD4435243.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIASYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKGSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61581..61985)
                     /gene="rcsF"
                     /locus_tag="KJL99_000365"
     CDS             complement(61581..61985)
                     /gene="rcsF"
                     /locus_tag="KJL99_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HBD4435244.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKQMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(62103..62918)
                     /gene="metQ"
                     /locus_tag="KJL99_000366"
     CDS             complement(62103..62918)
                     /gene="metQ"
                     /locus_tag="KJL99_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HBD4435245.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62958..63611)
                     /gene="metI"
                     /locus_tag="KJL99_000367"
     CDS             complement(62958..63611)
                     /gene="metI"
                     /locus_tag="KJL99_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HBD4435246.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVCKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63604..64635)
                     /gene="metN"
                     /locus_tag="KJL99_000368"
     CDS             complement(63604..64635)
                     /gene="metN"
                     /locus_tag="KJL99_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HBD4435247.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQTEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64823..65398
                     /gene="gmhB"
                     /locus_tag="KJL99_000369"
     CDS             64823..65398
                     /gene="gmhB"
                     /locus_tag="KJL99_000369"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HBD4435248.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
     gene            65762..>66032
                     /locus_tag="KJL99_000370"
     rRNA            65762..>66032
                     /locus_tag="KJL99_000370"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT        15630 a        16348 c        18196 g        15858 t
ORIGIN      
        1 tgggattgat atttatctgc gggaatttat attatgtttc tgcaaaaatg tttaaactaa
       61 aatttttatt aaggaaagag aggatgtatg attttcagaa aatatttata ttcgtaatat
      121 aaataaatta ccatttgctt aatttgtcaa atgctcttgt atgtaagact tcacgcaacg
      181 tctccccatc aggaaacgcc agctccggtg caatctcaaa cagcggccac agcataaatc
      241 cacgattctt catatcgtag tgcggaacgg tcaggcgttc ggtatttatc acttcattac
      301 caaacagcat gatatcgaga tccagcgtgc gtggtcccca gcgttcagct ttacgaacac
      361 gaccctgttg cagttctatg cgctgtgtat ggttgagtaa ctcttccggg gcgggcgaag
      421 tttcaagcgc cacggcggcg ttgaggtaat cgggttgatc ttgcggcccc agcggtgggg
      481 tgcggtaaaa cgaagaaacg gcaagaatgc ggctttcagg gatatcgcct aatgctttca
      541 gggcagcatt gacctgctcc agcggagagg ccagattgct gcctatggca atatacgcca
      601 ctgtcatgtg gtaccctcac gacgcggtgc gcgtttgcgt ggacgacgag tacgacgacg
      661 cggtgacggt tcttcatcca gctcgttaag catccctttt tgatctggtg gtgcggaaac
      721 ctggaactca ccccaccatt tcaccagacg ctgcagttca gcattacgtt caacttcagc
      781 tcgcaaggcc aacaggtcat aagccgcacg gaacttagga tgctccagca gtttccatgc
      841 gcgtttaccc tgacgacggg acatacgcaa ctgcaactgc cagatatcgc gggttaacgt
      901 cgtcagacgt ttcgggattg ccagtgaacg gcaggcttcg tccagcacgt cgttcatcgc
      961 cagcgcgaaa gcgtcgtgat aggtcaggcc gctttcctgg gcgatcttct gtgccgtctc
     1021 cagcagtggg taccagaaca tggcggcaaa caagaacgcc gggttcacgc gcatatcgtt
     1081 atggatacgc gtatcggtat tcttcagcac ctgttcaatg atccgctcca tcgggctgtc
     1141 gccattttcc gtgaagtagc gggtaatggt cgggaacagc ggctggaaca gatgatattc
     1201 acacaacagc ttataggttt cgtaaccgta gcccgcttgt agcagtttaa gcgattcttc
     1261 aaacaggcgt gccggtggga tatcgttcag cagggtagcg aggcgaggga tcggttctgc
     1321 ggtttccggg ctgatgcgca tacccaattt ggcggcaaaa cgtaccgcgc gcagcatacg
     1381 taccggatct tcacggtagc gcgtttccgg gttaccaatc agacggataa cgccgtcctt
     1441 cagatccttc ataccaccaa cgtaatcacg gacggtaaaa tccgctacgc tgtaatacag
     1501 gctgttgata gtgaaatcgc ggcgctgggc gtcttcttcg atggagccga aaatgttgtc
     1561 gcgcagcaac atgccgtttt gcccgcgttg ggaggtcgtg cggtcgctga cgttaccttc
     1621 gtggtgtcca cggaaggtcg caacttcgat aatctccggg ccaaacatta catgagccag
     1681 acggaaacgg cgacccacca ggcggcagtt acggaacagt ttgcgcacct gctcaggcgt
     1741 ggcgttagtg gttacgtcaa aatctttcgg ctttttgcca agtaacaggt cgcgcacgcc
     1801 gccgccaacc agccaggctt cgtatcccgc tttgttgagc ctgtacatta ccttcagggc
     1861 attttcactg atatctttgc gggaaatagc atgctgctca cgcgggatca ccgtcacctg
     1921 tggacgggcg actgcctgtt cagcctcgct ttcctcgcgg cttagcacct tgcggcaaaa
     1981 attagcgact cgggtaaaaa tagtacacct cggtagtgtc aaacatcatt caggacaaaa
     2041 aaatagcggc taatcatagc tcagcatgac gcatttgaga atgttgaatt tacaattgcc
     2101 gactcgggca cggcggtcag catccagttt ttgacggctg actgaaggat ttgctcgacg
     2161 ctgaaatcct gccagtgtgc ttctgcctgc tgccccagaa attgaagtgc cgcgattagt
     2221 accgggcgtg gatcgccttt cggcaacgca ggcgcatgat tctgcttgga aagtttagcg
     2281 ccttgtggat taagcgccag cggcagatga atgtaatctg gcactttcca gccaaaaagc
     2341 tggtacagcg ggatttgcct tactgttggt tcaatcagat cagccccacg cactatttct
     2401 gtaacgccct ggaaatgatc atcaaccaca acagccaggt tgtaggcgaa caacccatca
     2461 cggcgatgaa tgataaaatc ttcccgtgcc agtttttcgt cggcgtgaat aatgcctcgc
     2521 agcaggtcag taaattgcgt gaccggatgc tgctggcgga tacgcactgc ggcgttgtct
     2581 ggtccatgat gcaacacccg gcaatgaccg tcgtaaatac cgccaatgct ttgaatacgc
     2641 gcacgcgtac aggtgcagta ataacttagt ccttgttcat gtaaccaggc gagtgcttca
     2701 cgataggcgt cgtgacgttg cgattgccag agaacatcgc cgtcccagtg cagaccgtaa
     2761 tgttccagct ggcgcaggat agtttctgcg gcaccgggaa cttcacgagg cgggtcgata
     2821 tcttctatgc gtaccagcca gcgaccttgc cgggcgcgag cctgcaaata gctgccgagc
     2881 gcggcgatca gagagccaaa atgaagctcg ccggaaggag agggggcgaa gcggccaata
     2941 tactgtgtgt ctgtcatctc tttgaacaaa aaataaggcg ggagcatttc ccgcctgtgg
     3001 taaacgtgat ggaacggctg taattagcca gccatctgtt tttcgcgaat ttcagccagc
     3061 gttttgcagt cgatgcacag atcggctgtc gggcgcgctt ccagacggcg aataccaatt
     3121 tcaacaccgc aggattcgca gtagccgaaa tcttcgtctt ccactttttt cagcgtcttc
     3181 tcgatctttt tgatcagctt acgctcgcga tcgcggttac gcagttcgag gctgaactct
     3241 tcttcctggg ctgcacggtc taccgggtcc gggaagttgg ctgcttcatc ctgcatatgt
     3301 gtaacggtgc gatcgacttc atccctgagt tgattacgcc atgcttccag aatacgacgg
     3361 aagtgcgcca gctgggcttc attcatatac tcttcgcccg gcttctcctg atatggttcc
     3421 accccagcga tggcgagaat actcagggac gatgttttac ggttttgccc ttcttgcatg
     3481 ttgcttctcc ttaacacgca ctatcgatcc ccatgttcgg gggaaaaatg aggccgctat
     3541 aaatagcaga tgcttttccg gatagcaatt atctaaacgt aacacttgac aactgtgtga
     3601 ggaaaagcgt atttgcgcac gcgaccagaa tgtaaattaa ccagttactt actttactac
     3661 aatgtaaccg gcagtgattt tttaagagcc atgccttcag cagaaatttc cgctttgtaa
     3721 gccagaattt ctacccccct ctgttgagct tctgacaata gttgcgcgta tttctcatcg
     3781 atgtggcgcg cgggtgaaaa ccgtgtaatg gctgaatgca gcacggcgaa aaaaataacc
     3841 gcacgctggc cttcagccgc tacgctcatc aactcccgaa ggtgtttctg acctcgttca
     3901 gtgaccgcat cgggaaaata tccctgttcg ttctccgcta acgtaaccga tttcacttca
     3961 atatagcagt ctggacgcga atccgcctgc aacataaagt caatacggct gcgttcggag
     4021 ccgtatttta cttcgctttt cagcgagcta tagcctgaca gttctgaaat tgattcatta
     4081 aggatagcct ctttcgtcaa cctgttagcc caaagcgtat tgacgcaaat aaatgcgccg
     4141 ctctggcttt gagttaattc ccaggtgtgt gggtatttcc gtttggtgtt gtctgaagtc
     4201 gaataccaga cggtatcgcc aggcgttgca caaccggtca tcgcacccgt attcgggcag
     4261 tgtagcgtta attcgcgacc atcgggtgtg atcacatcgg ctaaaaaacg tttgtaacgc
     4321 tgaattagcg tcgcgcgctg tagaggggga gaaaattcca ttgcgacaat ccttgttatt
     4381 gcgttagcgc ccagcgtttt agcggcgtgt agcgtgtgcg tccacgggca aacgaggagg
     4441 cgtaaagggt gaactccgtc accgcataag accagttaaa acccggcggc gggaatgtca
     4501 ccgcctcgct ggcgtcgcgc aataaggtaa tatgtggatg aaacggacga ttgctttgaa
     4561 aacaaccgct gcgggcagcc tgtgaacgga gcatattcgc cagctggatt aagccgcgtg
     4621 gaggctgacg catccctaac cacaccacac gcgaacgcag ccattgtccg gcgtcatcaa
     4681 gcgtgagtgt gaaaccaggt tgacgaatcc gtccggctaa aagagaaagc gccttctctt
     4741 tctctgcgct cacttcgcct aaaaatgcca gagtcaggtg caaattatcg gcggcgaccg
     4801 gacgtcccgc ctcaggtggg aagtgtgtgg cgcgccaatg gataatctgt tcgcggattt
     4861 ctgcaggtaa gtcgatagca aagaacagac gttgcggttc agacatgtga ggcactcggt
     4921 tatgaattac cgcgatgcta caatgtggcg cgaagaatgt taaccctctg gagcgttttg
     4981 tgtcgtcgtt gcccgttgct gccgtcttac ctgaattact taccgctctc gattgtgcgc
     5041 cgcaggtatt attaagtgcg ccgaccgggg ccgggaaatc aacctggctg ccgctgcaac
     5101 tgctggcgca tcccggcatt aacgggaaaa ttatcctgct ggagccgcgt cgtctggcgg
     5161 cgcgtaacgt cgcgcaacgg ctggcggagc tgcttaacga aaagccaggc gataccgttg
     5221 gctaccggat gcgtgcgcaa aactgcgtcg ggctgaatac ccgcctggaa gtggttaccg
     5281 aaggcgtgct gacgcggatg atccagcgtg acccggaact gagcggtgtt ggattggtga
     5341 tccttgatga gtttcatgag cgcagtttgc aggcggattt ggcgttggcg ctgttactcg
     5401 atgtgcaaca aggtctgcgt gatgacctta aactgctgat tatgtcggct acgctggaca
     5461 atgaccgctt gcagcaaatg ctgccggaag cgcccgtcgt catctcagaa gggcgctcgt
     5521 ttccggttga acgccgttat ttaccgctgc ccgcgcatca gcgttttgac gaagccgttg
     5581 cggtagccac cgccgaaatg gtgcgtcagg aaagtggatc attactgtta tttttacctg
     5641 gcgtcggaga aattcagcgc gtgcaggaac aactggcttc gcgcatcggc agtgatgtgg
     5701 tgctctgccc gctgtatggc gtgttgtcac tgaacgatca gcgaaaagcg atcctcccgg
     5761 caccgcaagg gatgcgcaaa gtggtgctgg cgaccaatat tgctgaaacc agtttaacca
     5821 tcgaaggtat tcgtctggtg gtggattgtg cccaggagcg tgtggcgcgc tttgatccgc
     5881 gcacggggct tacgcggttg attactcaac gcgttagtca ggcatccatg acgcagcgtg
     5941 ccgggcgcgc cgggcgtctg gagccgggta tcagcctgca tttaatcgcc aaagaacaag
     6001 cagaacgcgc cgcggcgcaa agtgagccgg agatcttaca aagcgatctt tccggtttgc
     6061 tgatggaatt actgcaatgg ggatgcagcg atccggcgca gatgagctgg ctggatcaac
     6121 cgccaacggt gaatctactg gccgcgaaac gcctgttaca aatgctgggg gcgctggagg
     6181 gtgaacggct tagtgcgcaa gggcaaaaaa tggcagcgct gggtaacgat ccgcgtttag
     6241 cggcaatgct ggttaacgca aagagcgatg acgaagctgc taccgcggca aaaattgccg
     6301 ccattctcga agagccgcca cggatgggca atagtgacct gggcgtggcg ttttcgcgca
     6361 atcaaccagc ctggcagcaa cgtagtcagc aactgttaaa acgcttaaac gtacgcggcg
     6421 gtgaggcaga cagttcgctt atcgcgccgc tacttgccgg ggcgtttgcc gatcgcattg
     6481 ctcatcgccg tgggcaagat ggacgctatc aactggcaaa cagcatggga gcgatgctcg
     6541 atgccgacga cgcgctaagc cgccacgaat ggttgatcgc accgttatta ttgcagggca
     6601 gcgcctcgcc ggatgcgcgg attttactgg cgctaccggt cgatattgat gaattagtac
     6661 aacgctgtcc gcagctggtg cagcaatctg acaccgtgga atgggatgac gcgcaaggta
     6721 cgctgaaagc ctggcgtcgg ctacaaatcg gtcagttgac ggtgaaagtg cagccgctgg
     6781 cgaaaccgtc agaagacgag ttgcatcagg cgatgcttaa tggcatccgt gataaaggtt
     6841 taagcgtgct caactggacg gcggaagcgg aacagctacg cttgcgtttg ttatgcgccg
     6901 caaagtggtt gccggaatat gactggccag cggttgatga tgaaagtttg ttggcgacgc
     6961 tggaaacgtg gctgctgcca catatgaccg gcgtacattc actacgcggc ctgaaatcac
     7021 tcgacattta tcaggcacta cgcggattac ttgattgggg aatgcagcaa cgtctggata
     7081 gtgaattgcc tgcgcattac actgtgccga cgggaagccg gatcgccatt cgttatcatg
     7141 aagataaccc gcccgcgctg gcggtgagaa tgcaggagat gtttggtgag gccactaatc
     7201 cgacgatcgc ccaggggcgc gtgccgctgg tgctgaagtt gctttcccct gcccaaaggc
     7261 cgctgcaaat cacgcgtgat ttgggggcct tctggaaagg ggcgtatcgt gaggtgcaaa
     7321 aggagatgaa agggcgttat cccaaacacg tctggccgga cgacccggca aacacggcac
     7381 cgacgcgacg gacgaaaaag tattcgtaag gttggttttc tccctctccc tgtgggagag
     7441 ggccggggtg agggcatcag cgcgcacgtt caccctcacc ctaaccctct ccctcaaggg
     7501 agaggggacc gatcgagcgc aaattttgag agatatcttc ttctgtcttg taacagaaga
     7561 acagaaaatc gggcttttgc gcctgaatat tgcggagaaa aagcatggcc gggaatgacc
     7621 gcgagccaat tggacgcaaa gggaaaccga cgcgtccggt caaacaaaag gtaagccgtc
     7681 gtcgttacga agatgacgat gattacgacg attatgatga ctatgaggat gaagaaccga
     7741 tgccgcgcaa aggtaagggc aaaggcaaag ggcgtaagcc tcgtggcaaa cgcggctggc
     7801 tatggctact gctaaaactg gctatcgttt ttgccgtgct gatcgccatt tacggcgttt
     7861 atctcgatca aaaaattcgt agccgtattg atggcaaggt ctggcaactg cccgcggcag
     7921 tttatggccg aatggtcaac cttgaaccag atatgaccat cagcaagaac gaaatggtga
     7981 agctgctgga ggcaacacag tatcgtcagg tgtcgaaaat gacccgtcct ggcgaattta
     8041 ccgtgcaggc caacagcatt gagatgatcc gccgtccgtt tgatttcccg gacagtaaag
     8101 aagggcaggt gcgtgcgcgt ctgacctttg atggtgatca tctggcgacc atcgtcaata
     8161 tggagaacaa ccgtcagttc ggtttcttcc gtcttgatcc gcgtctgatc accatgatct
     8221 cctcgccaaa cggtgagcag cgtttgtttg tgccgcgcag tggtttccct gatttgctgg
     8281 tggatacttt gctggcgaca gaagaccgtc atttttacga gcatgatgga atcagtctct
     8341 actccattgg gcgtgcggtg ctggcaaacc tgaccgcagg tcgcacggtg cagggtgcga
     8401 gtacgctgac gcaacagctg gtgaaaaacc tgttcctctc cagcgagcgt tcttactggc
     8461 gtaaagcgaa cgaagcttac atggcgctga tcatggacgc gcgttacagc aaagaccgta
     8521 ttcttgagct gtatatgaac gaggtgtatc tcggtcagag cggcgacaac gaaatccgcg
     8581 gcttcccgct ggcaagcttg tattactttg gtcgcccggt agaagagcta agcctcgacc
     8641 agcaggcgct gttagtcggt atggtgaaag gggcgtccat ctacaacccg tggcgtaacc
     8701 cgaaactggc gctggagcga cgtaatctgg tgctgcgtct gctgcaacag caacagatta
     8761 ttgatcaaga actctatgac atgttgagtg cccgtccgct gggggttcag ccgcgtggtg
     8821 gggtgatctc tcctcagcca gcctttatgc agctggtgcg tcaggagttg caggcaaaac
     8881 tgggcgataa ggtaaaagat ctctccggcg tgaagatctt cactaccttt gactcggtgg
     8941 cccaggacgc ggcagaaaaa gccgccgtgg aaggcattcc ggcactgaag aaacagcgta
     9001 agttgagcga tcttgaaact gcgattgtgg tcgtcgaccg ctttagtggt gaagttcgtg
     9061 cgatggtcgg aggttctgag ccgcagtttg cgggctacaa ccgtgcgatg caggcgcgtc
     9121 gttcgattgg ttcccttgca aaaccagcga cttatctgac ggctttaagc cagccgaaaa
     9181 tctatcgcct gaacacgtgg attgccgatg cgccaatcgc gctgcgtcag ccgaatggcc
     9241 aggtctggtc accgcagaac gatgaccgtc gttatagcga aagcggcaga gtgatgctgg
     9301 tggatgcgtt gacacgttcg atgaacgtgc cgacggtaaa tctggggatg gcgctggggc
     9361 tgcctgcggt tacggagacc tggatcaaac tgggcgtacc gaaagatcag ttgcatccgg
     9421 ttccggcaat gctgctgggg gcgttgaact taacgccaat cgaagtggcg caggcattcc
     9481 agaccatcgc cagcggtggt aaccgtgcac cgctttctgc actgcgttcg gtaatcgcgg
     9541 aagatggtaa ggtgctgtat cagagcttcc cgcaggcaga acgcgctgtt ccggcgcagg
     9601 cggcgtatct gacactatgg accatgcagc aggtggtaca acgtggtacg ggtcgtcagc
     9661 ttggggcgaa atacccgaac ctgcatctgg cagggaaaac agggactacc aacaataacg
     9721 tagatacctg gtttgcaggc attgacggca gcacggtgac catcacctgg gtaggccgtg
     9781 ataacaacca gccgaccaaa ctgtatggag ccagcggggc aatgtcgatt tatcagcgtt
     9841 atctggctaa ccagacgcca acgccgctga atcttgttcc gccagaagat attgcagata
     9901 tgggcgtgga ctacgacggc aactttgttt gcagcggtgg catgcgtgtc ttgccggtct
     9961 ggaccagcga tccgcaatcg ctgtgccagc agagcgagat gcagcagcag ccgtcaggca
    10021 atccgtttga tcagtcttct cagccgcagc aacagccgca acagcaacct gctcagcaag
    10081 agcagaaaga cagcgacggt gtagccggtt ggatcaagga tatgtttggt agtaattaac
    10141 atctaagcgt gaaataccgg atggcgagtt gccatccggt aaaataacat cccatctaag
    10201 atattaaccc tttcttttca tctggttgtt tattaaccct tcaggaacgc tcagattgcg
    10261 taccgcttgc gaacccgcca gcgtttcgaa tattatctta tctttataat aatcattctc
    10321 gtttacgtta ttattcactt tacatcagag atataccaat ggcgcgttcc aaaactgctc
    10381 agccaaaaca ctcactgcgt aaaatcgcag ttgtagtagc cacagcggtt agcggcatgt
    10441 ctgtttatgc acaggcagcg gttgaaccga aagaagacac tatcaccgtt accgctgcac
    10501 ctgcgccgca agaaagcgca tgggggcctg ctgcaactat tgcggcgcgg cagtcagcta
    10561 ccggcactaa aaccgatacg ccgattcaaa aagtgccaca gtctatttct gttgtgaccg
    10621 ccgaagagat ggcgctgcat cagccgaagt cggtaaaaga agcgcttagc tacacgccgg
    10681 gtgtctctgt tggtacgcgt ggcgcatcca acacctatga ccacctgatc attcgcggtt
    10741 ttgcggcaga aggccaaagc caaaataact atctgaatgg cctgaagttg cagggcaact
    10801 tctataacga tgcggtcatt gacccgtata tgctggaacg cgctgaaatt atgcgtggcc
    10861 cggtttccgt gctttacggt aaaagcagtc ctggcggtct gttgaatatg gtcagcaagc
    10921 gtccgaccac cgaaccgctg aaagaagttc agtttaaagc cggtactgac agcctgttcc
    10981 agactggttt tgactttagc gatgcgttgg atgatgacgg tgtttactct tatcgcctga
    11041 ccggtcttgc gcgttctgcc aatgcccagc agaaagggtc agaagagcag cgttatgcta
    11101 ttgcaccggc gttcacctgg cgtccggatg ataaaaccaa tttcaccttc ctttcttact
    11161 tccagaacga gccggaaacc ggttattacg gctggttgcc gaaagaggga accgttgagc
    11221 cgctgccgaa cggtaagcgt ctgccgacag actttaatga aggggcgaag aacaacacct
    11281 attctcgtaa tgagaagatg gtcggctaca gcttcgatca cgaatttaac gacaccttta
    11341 ctgtgcgtca gaacctgcgc tttgctgaaa acaaaacctc gcaaaacagc gtttatggtt
    11401 acggcgtctg ctccgatccg gcgaatgctt acagcaaaca gtgtgcggca ttagcgccag
    11461 cggataaagg ccattatctg gcacgtaaat acgtcgttga tgatgagaag ctgcaaaact
    11521 tctccgttga tacccagttg cagagcaagt ttgccactgg cgatatcgac cacaccctgc
    11581 tgaccggtgt cgactttatg cgtatgcgta atgacatcaa cgcctggttt ggttacgacg
    11641 actctgtgcc actgttcaat ctgtacaatc cggtgaatcc cgatttcgac ttcaatgcca
    11701 aagatccggc aaactccggc ccttaccgca ttctgaataa acagaaacaa acgggcgttt
    11761 atgttcagga tcaggcgcag tgggataaag tgctggtcac cctaggcggt cgttatgact
    11821 gggcagatca agaatctctt aaccgcgttg ctgggacgac caataaacgt gatgacaaac
    11881 agtttacctg gcgtggtggt gttaactacc tgtttgataa tggtgtaaca ccttacttca
    11941 gctatagcga atcgtttgaa ccttcttcgc aagttgggaa ggatggtaat attttcgcac
    12001 cgtctaaagg taagcagtat gaagtcggcg tgaaatatgt accggaagat cgtccgattg
    12061 tagttactgg tgccgtgtat aatctcacta aaaccaacaa cctgatggcg gaccctgagg
    12121 gttccttctt ctcggttgaa ggtggcgaga tccgcgcacg tggcgtagaa atcgaagcga
    12181 aagcggcgct gtcggcgagt gttaacgtag tcggttctta tacttacacc gatgcggaat
    12241 acaccaccga tactacctat aaaggcaata cgcctgcaca ggtgccaaaa cacatggctt
    12301 cgctgtgggc tgactatacc ttctttgacg gtccgctttc aggtctgacg ctgggcaccg
    12361 gtggtcgtta tactggctcc agctatggtg atccggctaa ctcctttaaa gtgggaagtt
    12421 atacggtcgt ggatgcgtta gtgcgttatg atctggcgcg agtcggcatg gcgggctcca
    12481 acgtggcgct gcatgttaac aacctgttcg atcgtgaata cgtcgccagc tgctttaaca
    12541 cttatggctg cttctggggc gcagaacgtc aggtcgttgc aaccgcaacc tttcgtttct
    12601 aatttctctt ttggggcacg gatttccgtg cccatttcac aagttggctg ttatgcagga
    12661 atacacgaat cattccgata ccacttttgc actgcgtaat atctcctttc gtgtgcccgg
    12721 gcgcacgctt ttgcatccgc tgtcgttaac ctttcctgcc gggaaagtga ccggtctgat
    12781 tggtcacaac ggttctggta aatccactct gctcaaaatg cttggtcgtc atcagcctcc
    12841 gtcagaaggg gagattcttc ttgatgccca gccgctggaa agctggagca gcaaagcgtt
    12901 tgcccgcaaa gtggcttatt tgccgcagca gcttcctccg gcagaaggga tgaccgtgcg
    12961 tgaactggtg gcgattggtc gttacccgtg gcatggcgcg ctggggcgct ttggtgcggc
    13021 agatcgcgaa aaggtcgagg aagctatctc gctggttggc ttaaaaccgc tggcgcatcg
    13081 gctggtcgat agtctctctg gcggcgaacg tcagcgggcg tggatcgcca tgctggtggc
    13141 gcaggatagc cgttgtctgt tgctcgacga accgacttca gcgctggata tcgcccacca
    13201 ggttgatgtg ctggcgctgg tgcaccgttt aagtcaggaa cgtggcctga cggtcattgc
    13261 cgtgttgcac gatatcaata tggcggcacg ctactgtgat tatctggtcg ccctgcgcag
    13321 tggtgaaatg attgctcagg gaacgcctgc ggaaattatg cgcggcgaaa ccctcgaaat
    13381 gatttatggc atcccgatgg gtattttgcc gcatccggcg ggtgctgcac ctgtgagttt
    13441 tgtttattga tgagcggctt acctcttatt tcgcgccgtc gactgttaac ggcgatggcg
    13501 ctttctccgt tgttatggca gatgaatacc gcccacgcag cggctattga ccccaatcgt
    13561 attgtggcgc tggagtggtt gccggtggaa ttactgctgg cgctcggcat cgtgccttac
    13621 ggcgtggcgg ataccatcaa ctatcgcctg tgggtcagcg aaccaccatt gccggactca
    13681 gtgattgacg tcggtttgcg tacagaacct aaccttgaac tgctgaccga aatgaaaccg
    13741 tcgtttatgg tctggtcggc aggatatggc ccttcatcag aaatgctggc acgtattgcg
    13801 ccgggtcgcg gatttaactt cagtgacggc aaacatccgt tggcgatggc gcgtaaatcg
    13861 ctgacggaaa tggcagattt acttaacctg caaagcgcag cggaaacgca tttagcgcaa
    13921 tacgaagact ttatccgcag catgaaaccc cgctttgtga agcgtggtgc acgcccgtta
    13981 ctgctgacga cgcttatcga tccgcgccat atgctggtct tcggtccaaa cagcttgttc
    14041 caggaaattc tcgatgagta cggcatccca aatgcctggc aaggggaaac caacttctgg
    14101 ggcagtaccg ccgtcagtat cgatcgtctg gcagcgtata aagacgttga tgtgctctgt
    14161 tttgatcacg acaacagcaa agacatggat gcgctaatgg caaccccgct gtggcaggcc
    14221 atgccgtttg tccgtacagg gcgctttcag cgcgtacccg cagtctggtt ttatggtgca
    14281 acgctttcgg caatgcactt tgtgcgtgtt ctggataacg ccatcggagg taaagcgtga
    14341 gtaaacgaat tgcgcttttc ccggcgttat tgctggcgct gttagtgatt gtcgctacgg
    14401 cgctcacctg gatgaacttc tcacaggcgc tgccgcgcag tcagtgggcg caggccgcct
    14461 ggtcgccgga tattgacgtc atcgagcaga tgatttttca ctacagcttg ttgccgcgtc
    14521 tggcgatttc gctgctggtg ggcgcgggcc tggggctggt gggcgtgctg tttcagcaag
    14581 tactgcgtaa cccgctggcg gaaccgacga cgttgggcgt tgcaacaggc gcgcaactgg
    14641 ggattaccgt caccacgctc tgggcgatcc ctagcgcgat ggcgagccag tttgctgcgc
    14701 tggcaggggc ttgcgttgtt ggcttaattg tctttggcgt cgcgtggggg aaacggctgt
    14761 cgccggtaac gctgattttc gcggggttgg tagtgagcct ttattgcggc gcaatcaatc
    14821 agttactggt tatcttccat catgaccagt tgcaaagcat gtttctgtgg agcactggaa
    14881 cgctgacgca aaccgactgg ggcggtgttg agcgtttatg gccgcagttg ctgggcggtg
    14941 tgatgctgac gttgctgcta cttcgtccgt taaccctgat ggggcttgat gatggcgtgg
    15001 cgcgcaatct cgggctggcc ttgtcgcttg cgcgtctggc ggcgctgtcg ctggcgattg
    15061 tcatcagtgc gctgctggtg aacgctgtgg ggattatcgg ctttatcggg ttgttcgcgc
    15121 cgctgctggc aaaaatgctg ggggcgcggc gtctgctgcc acgactgatg ctggcgtcgt
    15181 tgattggtgc gctgatcctc tggctttccg atcaaatcat cctctggctg actcgcgtgt
    15241 ggatggaagt gtccaccggt tcggtcactg cgttgatcgg tgcgccgctg ctactgtggc
    15301 tgctgccgcg tttacgcagc attagcgcgc cggatatgaa ggttaacgat cgtgtcgcga
    15361 ctgaacgcca acatgtgctg gcgtttgccc tcgcgggcgg cgtgctgttg ttgatggctg
    15421 tggtggtggc gctgtcgttt ggtcgtgatg cgcacggctg gacgtgggcg agcggggcgt
    15481 tgctcgagga tttaatgccc tggcgctggc cgcgaattat ggcggcgctg tttgcgggcg
    15541 tcatgctggc ggtagcgggc tgtattattc agcgactgac cggcaacccg atggcaagcc
    15601 cggaagtgct ggggattagc tccggcgcgg cgtttggtgt ggtgttaatg ctgtttctgg
    15661 taccgggtaa tgcctttggc tggttgttac ccgcaggcag tctcggggcg gcggtgacgc
    15721 tgttgatcat tatgatcgcc gccggacgcg gtggattttc cccacaccgc atgttactgg
    15781 cggggatggc gttaagtacc gcgttcacca tgcttttgat gatgttgcag gcaagtggtg
    15841 acccgcgaat ggcgcaagtg ctgacctgga tttccggttc gacctacaac gcgaccgatg
    15901 ctcaggtctg gcgcaccgga attgtgatgg tgattttgct ggcgattact ccgctgtgcc
    15961 gccgctggct gaccatttta ccgctgggtg gtgataccgc gcgggcggta gggatggcgc
    16021 tgacgccgac gcgaattgcg ctgctgctgt tagcggcttg cctgacggcg accgcgacga
    16081 tgactattgg accgttgagt tttgttggtt taatggcacc gcatattgcg cggatgatgg
    16141 gctttcgacg gacgatgcca cacatcgtaa tttcggcgct ggtgggtggt ttactgctgg
    16201 tgttcgctga ctggtgtggg cggatggtgc tgtttccatt ccagatcccg gcggggctgc
    16261 tgtcgacctt tatcggtgcg ccatatttta tctatttgct gagaaagcag agccgttaat
    16321 agaatgcctc gcccggtggg cgaggcaaaa ttatcacaac ttcgcgaaca cccgacgtgc
    16381 agcatcgatg gtgttattga tatcttccat gctgtgcgcc acagacataa agcctgcttc
    16441 aaacgctgac ggtgccaggt aaacaccttc atccagcatc atatggaaga aacgcttaaa
    16501 acgctcgacg tcacaggcca tcacatcctg atagcacgtc acggactcgg cgtcggtaaa
    16561 gaagataccg aacataccac cgacgtggtt aacgaccagc ggaattcccg cttcttctgc
    16621 cgcttccagc agaccttctg ccagacgtgt tgtcagctca tccagcgttt cgtgaatgcc
    16681 cggctgcgcg acttcattca gacaggcgaa acccgctgcc atcgcaatcg ggttaccgga
    16741 aagcgtaccc gcctgataga ccggacctgt cggggccagc gcatccatta catcacgacg
    16801 accaccgaat gcgcctaccg gcattccacc gccgatgatt tttccgaggc aggttaaatc
    16861 tggcactacg gcgaaataat cctgtgcgcc agctagcgct acgcggaaac cggtcatcac
    16921 ttcatcgatg atcagcaacg cgccaaattc gtcgcacagc gcacgcagac ctggcaggaa
    16981 ctctggcagc ggcggaacac agttcatatt gcctgccacc ggctcgacga taatacaggc
    17041 aatctcttgc gggtattgct caaacgcggc gcgtacagag gccagatcgt tataagtaca
    17101 ggttaaggta tgtttggcga aatctgccgg aacgcccggc gagtttggct ggcctaacgt
    17161 gagtgcgcca gaaccggctt tcaccagcag gcagtcagcg tgaccgtggt aacaaccttc
    17221 aaatttaatg attttgtcgc gaccggtaaa accacgggcc aggcggatgg cgctcatcgt
    17281 cgcctcggtg ccggagttca ccatgcgcac catatccatg gtcgggacca gttcggtcac
    17341 cagttgcgcc attttcactt ccatttcggt cggtgcacca aagcttaaac cacgctcggc
    17401 ggcttcaatc acggcattgc ggatcgccgg atggttatgg cccagcacca tcggccccca
    17461 ggaaccgaca taatcgatat aggctttgcc atcaacatcg tacagataag cgccgtccgc
    17521 tttttcgata aacagtggag tgccgcccac gccagtaaag gcgcgaacag gggagttcac
    17581 accgccaggg atcagctcgc gcgctgcgct gtaaagattt tcagacttac tcatggaggg
    17641 ttcctgattc gtagaaaaag tgaatggctg ctattccatg ttattcataa caagttaaat
    17701 actcgtcaaa catcaggctg cttgtactgg tgcaatcctg atttcttaga gtataaaagt
    17761 tttgtgcatt tgaaacatta cgctttgcaa aggattttca tggaacgtgc gagtaaaatg
    17821 ccgtcatctt atttgtatga ccaataagtg atcattggat gaaaactgat actccctctt
    17881 tagaaacacc gcaggccgcg cgcctgcgac gcagacaact gattcgccaa cttcttgagc
    17941 gcgataaaac cccgttagcc attttgttta tggcggcagt cgtcggcacg cttgtcgggc
    18001 tggctgcggt tgcttttgac aaaggtgtcg cctggttgca gaaccaacgt atgggggcgc
    18061 tggtacatac tgctgataat tatccgctac tgttaaccgt cgcttttctc tgttcggcgg
    18121 tgctggcgat gttcggctac tttttggtgc gcaaatacgc gcccgaagca gggggatcgg
    18181 ggatcccgga aattgaaggg gcgctggaag atcaacgtcc tgttcgctgg tggcgtgtat
    18241 tgccggtgaa attctttggc gggctgggga cactcggcgg aggcatgggg ctagggcgcg
    18301 aaggaccaac cgtgcagatc ggcggcaata tcagtcgcat ggtgcttgat attttccgcc
    18361 tgaaaggtga tgaagctcgc catacgctgc tggcaaccgg tgctgctgcg gggctggctg
    18421 cggcctttaa cgcgccgctg gcgggtattt tgtttattat cgaagagatg cgtccgcagt
    18481 ttcgctatac gttaatttcg attaaagcgg tatttattgg cgtcattatg tcgaccatta
    18541 tgtaccggat ttttaatcat gaagttgcgt tgattgacgt cggtaaactt tctgatgcgc
    18601 cgcttaatac gctgtggctt tatctgatcc tcggtattat ttttggcatt ttcggcccta
    18661 tttttaataa atgggtgctg gggatgcagg atttgctgca ccgtgtgcac ggcggcaata
    18721 ttaccaaatg ggtactgatg gggggggcga ttggcggtct gtgtggattg ctggggtttg
    18781 tggcaccagc aacgtcgggc ggcggtttta acctgattcc tatcgctacc gcggggaatt
    18841 tcagcatggg aatgctggtg tttatcttcg tcgctcgggt cattaccacc ttactctgct
    18901 tctcttccgg cgcgccgggc ggtatttttg ccccgatgct ggcgctgggt actgtgctgg
    18961 gaaccgcttt cggaatggtt gccgttgagc tgtttccgca atatcacctg gaggcgggga
    19021 cgtttgctat tgccggaatg ggggcattac tggcggcatc tattcgcgcg ccgttaacgg
    19081 ggatcattct ggttctggag atgaccgata actaccagct cattttgcca atgattatta
    19141 ccggtcttgg cgcaacacta ttagcgcaat ttaccggcgg gaaacctcta tactcggcga
    19201 ttcttgcgcg cacgctggca aaacaggaag ctgagcaact ggcgcgaagc aaggccgcat
    19261 cagccagcga gaatacttga acgaaatacc agggtattag ataatggcga ttattattgg
    19321 gttagaattt gcccgattgc cgatgtcgtt tggagcaaaa tatgagtgat gacgtagcac
    19381 tgccgctgga gtttaccgac gcagcagcca acaaagttaa aagcctgatc gctgacgaag
    19441 ataacccgaa tctgaaatta cgcgtgtata tcaccggtgg cggttgcagc ggcttccagt
    19501 atggtttcac ctttgatgat caggtgaacg aaggcgatat gaccatcgaa aaacagggcg
    19561 ttggcctggt ggttgatccg atgagcctgc aatatctggt cggcggttcc gttgactaca
    19621 ccgaaggact ggaaggttct cgtttcatcg tgaccaaccc gaacgcgaaa agcacctgcg
    19681 gttgcggttc ttcctttagc atctaatctg ttgttcatgt gccggataaa ctatccggca
    19741 catcttctct cttcaacgcc cattctcatc cagcgcaaac gtcggtagct taagatgcca
    19801 acgaatagcc gccagccgaa tcaatagcgt cacgaccatg cccatcatac tggctgtttc
    19861 cagtggtacg gaaaatgtgt aataagccgt agcgtggaca ataccgccga taatacaggc
    19921 agttgcgtag atttctgtac gtaaaatcat ggggatttcg cggaccagta catcacgaat
    19981 gatcccgccg ccaacgccag taatgacgcc catacaaacc gcgattaacg gaccggcttc
    20041 cgcattaaag gctttattca cgctaatgcc gacaaacacc gccagaccaa cggcgtccag
    20101 caccggcaac atccattttg gtaagcgtct tggctggcgc accagcacga tggtcagcat
    20161 gctggtgacc atggcaacga ccagatcggt gggatctttc acccaaaata ccgggccgtg
    20221 atccagcgcc atgtcgcgaa ttgttccgtc gcctactgcg gtaaccacgc ccagtaccag
    20281 aacaccaaaa gggtccatac gcaattttcc ggctaacaaa acgccgtaga tggcaaatac
    20341 cgctgtgccg actatatcca gccaatagac gagcattgtt caatccccac tgagcacctg
    20401 aaaaggtcag gcactaatct acctgtgaaa gcgcattaca gagctgttgt gctgcgagga
    20461 taatacgtgg gcttgcacgt tcaaaccagt cactcgtgag aggaataacg ggaattttga
    20521 gctgttcacc ccagtattgt ttgattttag gaatttggtc cggtccgcct gtaatgacaa
    20581 tagcctgtgg cgagcgtgct aacacctgtt cgcggctaac ttgcggccag ggaacccggc
    20641 tgtctttaaa gatgttttct ccgccacaaa cttcgagtac caggttctga atcgactctt
    20701 ttccactggt aaatggcgga ttaatgccga attgcagaaa aacacgtttt ttaggtttat
    20761 cagcatattg cgctttcaat tgcacgtact gatccagcag ggattgcgcg gcttgttcgg
    20821 ccttgtctgg ttgcggactc cagggggcca gttgacgtaa cgcattggca atttgttcaa
    20881 tgcttgtcgc atcgacccac atcactttta ttcccagcga agccagctgg tcaacctgcc
    20941 gctcggcatt acctccacgc caggcaatca ccagatcggg tttcagcgcg acaatgcgtt
    21001 ccagattcat cccctgccag gtggaaacct gctcaatctt ttgcgcttgt ggaggatagt
    21061 cggaatagct gctgacccca accggcgtga tcccggcggc aaaggcaagt tcagtgttgg
    21121 cgggagaaag cgtgatgacg cgcggcgcgg cgttgagcca cagtggcgcg agaaaagaca
    21181 gggcgaccag cgccctgaac agtgatttag ccatgtgcca gtttctgcac cagtgactca
    21241 accatcaggc tggactgttt agcggcaaca gccaggaact catcgaagct aagatgagac
    21301 tgttgatcgg ccacgtcgga gatggcgcgt acgacaacaa acgggacgtt gaaattgtgg
    21361 cagacatggg cgattgccgt cgcttccatc tctacagcaa tggcctgtgg gaagttgtgg
    21421 cggattttcg ccagaccaac agaaccgttg atgaaagcgt cgccgctaac aatcaggcca
    21481 cgtacagcgt taagattcag ttcggcaatg caggcctcag cggcagcgat cagtttatcg
    21541 tcagctttaa agcctgccgg acagcctggt aactgaccgt attcataacc aaatgccgtg
    21601 acatccgcgt cgtgataacg cgcttcgtcc gagacaacga tatcgcccac tttcagcgtt
    21661 ggtgccaggc caccggcaga accggtgtta ataatcacat ctggcttgca gtgttccaac
    21721 agcagagtgg cacccagcgc cgcagcgact ttaccgatgc ccgatttcag aagcgcaacc
    21781 tcggttccat tcagttggcc ggtatagatt tcgcaaccgc cgagactgat agtttgacgg
    21841 ttttcgattt tgtcacgcag cagcgtaact tcttcttcca ttgcaccaat gatgccgatt
    21901 ttcatagatt tactcgcgat aagcccgatt tgaaggcata gtttaccatg cgcttacggg
    21961 gaagcgtatt tctcacgcgg gagaggacat ggcacagatt gatttccgaa aaaaaataaa
    22021 ctggcatcgt cgttaccgtt caccgcaggg cgttaaaacc gaacatgaga tcctgcggat
    22081 cttcgagagc gatcgcgggc gtatcatcaa ctctccggca attcgtcgtc tgcaacaaaa
    22141 gacccaggtt tttccactgg agcgcaatgc cgccgtgcgc acgcgtctta cccactcgat
    22201 ggaagtccag caggtgggac gctacatcgc caaagaaatt ttaagccgtc tgaaagagct
    22261 taaattgctg gaagcatacg gcctggatga actgaccggc ccctttgaaa gcattgttga
    22321 gatgtcatgc ctgatgcacg atatcggcaa tccgccgttt ggtcattttg gcgaagcagc
    22381 gataaatgac tggtttcgcc aacgtttgca cccggaagat gccgaaagcc agcctctgac
    22441 tgacgatcgc tgcagcgtgg cggcactacg tttacgggac ggggaagaac cgcttaacga
    22501 gctgcggcgc aagattcgtc aggacttatg tcattttgag gggaatgcac aaggcattcg
    22561 tctggtgcat acattgatgc ggatgaatct cacttgggca caggttggcg gtattttaaa
    22621 atatacccgt ccggcgtggt ggcgtggcga aacgcctgag acacatcact atttaatgaa
    22681 aaagccgggt tattatcttt ctgaagaagc ctatattgcc cgcttgcgta aagaacttaa
    22741 tttggcgctt tacagtcgtt ttccattaac gtggattatg gaagctgccg acgacatctc
    22801 ctattgtgtg gcagaccttg aagatgcggt agagaaaaga atatttaccg ttgagcagct
    22861 ttatcatcat ttgcacgaag cgtggggcca gcatgagaaa ggttcgctct tttcgctggt
    22921 ggttgaaaat gcctgggaaa aatcacgctc aaatagttta agccgcagta cggaagatca
    22981 gttttttatg tatttacggg taaacaccct aaataaactg gtaccctacg cggcacaacg
    23041 atttattgat aatctgcctg cgattttcgc cggaacgttt aatcatgcat tattggaaga
    23101 tgccagcgaa tgcagcgatc ttcttaagct atataaaaat gtcgctgtaa aacatgtgtt
    23161 tagccatccg gatgtcgagc agcttgaatt gcagggctat cgggtcatta gcggattatt
    23221 agagatttat cgtcctttat taagcctgtc gttatcagac tttactgaac tggtagaaaa
    23281 agaacgggtg aaacgtttcc ctattgagtc gcgcttattc cacaaactct cgacgcgcca
    23341 tcggctggcc tatgtcgagg ctgtcagtaa attaccgtca gattctcctg agtttccgct
    23401 atgggaatat tattaccgtt gccgcctgct gcaggattat atcagcggta tgaccgacct
    23461 ctatgcgtgg gatgaatacc gacgtctgat ggccgtagaa caataaccag gcttttgtaa
    23521 agacgaacaa taaattttta ccttttgcag aaactttagt tcggaacttc gggctataaa
    23581 acgaatctga agaacacagc aattttgcgt tatctgttaa tcgagactga aatacatgaa
    23641 aaaaaacaca ttagcactga gtgcactggc tctgagttta ggtttggcgt tatctccgct
    23701 ctctgcaacg gcggctgaga cttcttcagc aacgacagcc cagcagatgc caagccttgc
    23761 accgatgctc gaaaaggtga tgccttcagt ggtcagcatt aacgtagaag gtagcacaac
    23821 cgttaatacg ccgcgtatgc cgcgtaattt ccagcagttc ttcggtgatg attctccgtt
    23881 ctgccaggaa ggttctccgt tccagagctc tccgttctgc caggggggcc agggcggtaa
    23941 tggcggcggc cagcaacaga aattcatggc gctgggttcc ggcgtcatca ttgatgccga
    24001 taaaggctat gtcgtcacca acaaccacgt tgttgataac gcgacggtga ttaaagtcca
    24061 gctgagcgat ggtcgtaagt tcgacgcgaa gatggttggc aaagatccgc gctctgatat
    24121 cgcgctgatc caaatccaga acccgaaaaa actgaccgca attaagatgg cggattctga
    24181 tgcactgcgc gtgggtgatt acaccgtagc gattggtaac ccgtttggtc tgggcgagac
    24241 ggtaacttcc gggattgtct ctgcgctggg gcgtagcggc ctgaatgctg aaaactacga
    24301 aaacttcatc cagaccgatg cagcgatcaa ccgtggtaac tccggtggtg cgctggttaa
    24361 cctgaacggc gaactgatcg gtatcaacac cgcgatcctc gcaccggacg gcggcaacat
    24421 cggtatcggt tttgctatcc cgagtaacat ggtgaaaaac ctgacctcgc agatggtgga
    24481 atacggccag gtgaaacgcg gtgagctggg tattatgggg actgagctga attccgaact
    24541 ggcgaaagcg atgaaagttg acgcccagcg cggtgctttc gtaagccagg ttctgcctaa
    24601 ttcctccgct gcaaaagcgg gcattaaagc gggtgatgtg atcacctcac tgaacggtaa
    24661 gccgatcagc agctttgccg cactgcgtgc tcaggtgggt actatgccgg tgggcagcaa
    24721 actgaccctg ggcttactgc gcgacggtaa gcaggttaac gtgaacctgg aactgcagca
    24781 gagcagccag aatcaggttg attccagctc catcttcaac ggcattgaag gcgctgagat
    24841 gagcaacaaa ggcaaagatc agggcgtggt agtgaacaac gtgaagacgg gcactccggc
    24901 tgcgcagatc ggcctgaaga aaggtgatgt gattattggt gcgaaccagc aggcagtgaa
    24961 aaacatcgct gaactgcgta aagttctcga cagcaaaccg tctgtgctgg cactgaacat
    25021 tcagcgcggc gacagcacca tctacctgtt aatgcagtaa tctccctcaa ccccttcctg
    25081 aatacgggaa ggggttctcc ttacaatctg tgaacttcgc cacaactcca tacatcttca
    25141 tcatcctttg ggcatttgca caatgccgta cgttacgtac ttccttatgc taagccgtgc
    25201 ataacggagg acttatggct ggctggcatc ttgataccaa aatggcgcag gatatcgtgg
    25261 cacgtaccat gcgcatcatc gataccaata tcaacgtaat ggatgcccgc gggcgaatta
    25321 tcggcagcgg cgatcgtgag cgtattggtg aattgcacga aggtgcattg ctggtacttt
    25381 cacagggacg agtcgtcgat atcgatgacg cggtagcacg tcatctgcac ggtgtgcggc
    25441 aggggattaa tctaccgtta cggctggaag gtgaaattgt cggcgtaatt ggcctgacag
    25501 gtgaaccaga gaatctgcgt aaatatggcg aactggtctg catgacggct gaaatgatgc
    25561 tggaacagtc gcggttgatg cacttgttgg cgcaggatag ccgtttgcgg gaagaactgg
    25621 tgatgaacct gattcaggca gaggagaata ctcccgcact tctgaatggg cgcaacggct
    25681 ggggatcgat ctcaatcaac cgcgagtggt ggctattgtt gaggtcgaca gcggtcagct
    25741 tggcgtggac agcgcaatgg cggagttaca acaactgcaa aacgcgctga ctacgcccga
    25801 gcgtaataat ctggtggcga ttgtctcgct aaccgaaatg gtggtgttga aaccggcgtt
    25861 gaactctttt gggcgctggg atgcagaaga tcatcgtaag cgagttgaac aactgattac
    25921 ccgcatgaaa gagtacggcc agctgcgttt tcgcgtttca ctgggcaact attttaccgg
    25981 tcctggcagt attgcccgat cctatcgtac ggcgaaaacg acgatggtgg tgggtaaaca
    26041 gcggatgcca gaaagtcgct gctattttta tcaggatctg atgttacctg tgttactcga
    26101 cagtttgcgt ggcgactggc aggccaacga actggcgcga ccgctggcgc ggctgaaagc
    26161 gatggacaat aacggcttgc tgcgacgaac gctggcggcg tggtttcgcc acaatgtgca
    26221 accgctggca acgtcaaagg cgttgtttat tcatcgtaat accctggagt atcggcttaa
    26281 tcgtatatcg gaactgaccg ggcttgattt gggcaatttt gatgacaggt tgctgctgta
    26341 tgtggcgtta caactggatg aagagcggta gattatgcgt taaggttgag gcggcgctgg
    26401 ctcattcccc tcagcccgaa ggggagaggg gaatgcccga gttaaccgtc aattcgggat
    26461 tatttacgcg ttagtttttc cagatcggct tcaatctcgc tgatcttatt ggttacgact
    26521 gactccaggt gacgtaagtc gtcgaggatc ttacgcttaa gatcaacttc gctgcggtca
    26581 cgctgacaga tttgatcaag ctcatcaatg atataccgta gattcgggct gatttcctgg
    26641 acttctttat aaccctgacc cacaccatca gcgacgaccg ttttacgctg acgcggatat
    26701 ttaaacttaa cgctcttggc gaaaaactcg cctttgtctt tctggaaata gattttcaga
    26761 atatcgttgt tggcttcctg ccggaggctg taacgatcaa tttcttcagg attggtaata
    26821 cccagacttt tcagattgtc gtacatagcg ttacctcaaa atgagtcagt aaattgtgct
    26881 tattttagca tttggcttgg cccgcccggc tcgactgtga tcgcagaaag cctttttctt
    26941 ccagacttct atcatggcgt aaaaatcaaa aaattacctg ctttattgtg gcgatattcc
    27001 atgggatgga ttagcacatt tatcttcgtt ttgcgaatta catgacagtc cgaaacaaga
    27061 tgaaaaagta tttaaaaaag ggggaggaag aatgagtagg acgaattatt ctgagccaga
    27121 aagcatacgg gcagcatcat gctgcccgta tatgtactct taatcgatgg tacgcagcag
    27181 ttcgttaatg ccgactttgc cgcgagtttt cgcgtcaact ttcttaacga taaccgcaca
    27241 gtagaggctg tatttgccat cttttgacgg cagattacct gaaacaacca cagaccccgc
    27301 cggaacgcga ccgtagtgga tttcgccggt ttcacggtcg taaatacggg tgctctgacc
    27361 aatgtatacg cccatagaga tgacggaacc ttcttcgaca atcacccctt caaccacttc
    27421 agagcgtgcg ccaatgaagc agttatcttc aataatggtc ggggtagcct gcagcggctc
    27481 cagcacgccg ccgatgccca cgccaccgga aaggtggacg tttttaccaa tctgcgcaca
    27541 agaaccgacg gtcgcccagg tatcaaccat ggtgccttca tcaacatatg cgccgatgtt
    27601 aacgtaagac ggcatcagca cggtgttacg ggcaataaac gcaccctgac gtaccgccgc
    27661 tggtggcaca acgcggaagc cttctttctg gaaacgtgct tcgtcgtagt cggcgaattt
    27721 catcggcact ttgtcgaagt agcggctttc tgccccttcg atcacctgat tatcattaat
    27781 acggaaagag agcagcaccg cttttttcaa ccactgatgc gtcacccact gaccgtcaat
    27841 tttttccgct acacgcagtg cgccggaatc cagcagggcg atcacctgat ttaccgcttc
    27901 gcgggtaacg gtgtctgcat tggctggcgt gatctcggca cggcgttcaa aagcggtttc
    27961 aataatgttc tgtaactgct gcattgttaa actcttttca tatcagtaaa cacatcaccc
    28021 tttatcgttt ggattgaggg cctctgtcaa ccgctgatgc acttcctgct gcaactcgtt
    28081 attaagcgca cgccggtcag cggtggcaat tatgaataaa tcttctactc gctcgccaat
    28141 ggttgtaatt cgggcaccat gaagcgaaat tcccagatcg gcaaaaattt tcccgactcg
    28201 cgccagcagt ccaggttggt cgagggcgat cagttcgagg aacgatttgc ggtcggtatg
    28261 ggtcggcaaa aacgttactt cggtttcaac agtaaaatgg cgtaatttgg cgggttggcg
    28321 acggggctgc ggtggctgcc agctactttg cgtcagcacc tgctccagac caaaccgaat
    28381 aacctcatgg cgatctgcgg acagcgggct gccatcgggt tccagcacga taaaggtatc
    28441 catcgccata ccgtcgcgag tggtgaaaat ttgtgcgtcg tgaacactta aattgcggcg
    28501 gtctaattcg gcacagacgg cggcaaacag ataggggcgg tccgggctcc agataaaaat
    28561 ctcggtgcct ccacgcgtag cctgcgggct aagcaatacc agcggtttgc ttaaatcatg
    28621 ctgtaataaa tggcgggcat gccaggccag ttgatttggg ctatggcgga caaaatagtt
    28681 agcacgacag cgtgaccaaa tttggtgcag cgcctcttcg tcgatgttat ccatgcgcag
    28741 tagtgccagt gcctggagtt ggtgatggcg aacccgttcg cgcatatccg gcgtgttttg
    28801 cattccgcgt cgtagctgct tttcggtggc aaaatagagt tcgcgcaaca gactttgctt
    28861 ccagctattc cacagtgttt cgttggtggc gcaaatgtca gccactgtca ggcataccag
    28921 atagcgcaga cgattttccg tttgaacttc ttcggcaaac tgcttgatga cttcagggtc
    28981 ctgaatatcg cggcgttgtg ctgtcaccga catcagcaag tgctggcgaa ccagccaggc
    29041 aaccagctgt gtttcgcgtg agttcagccc gtggagttct gcaaaatgca ctacatcctg
    29101 agcaccgaga atggagtggt cgccgccgcg tcctttggcg atatcgtgaa acagcgcggc
    29161 gatgaaaatc agctcagttg acggcaggcg cggccagacg tccacacaca acggatggcg
    29221 ctggcgcgtt tcttcactgg caaaactctc cagtttcagc atcacgcgga tagtatgttc
    29281 atccaccgtg taggcgtgga acagatcaaa ctgcatctgc ccgacgatat gcgaccattg
    29341 cggcatatac gcgccgagca cgctatggcg atgcattggc aatagtccgc gccgcaccgc
    29401 tccggggtga cgcagaatgc tcaaaaacag ttttcgtgct tgcggaatat tacacagcgg
    29461 ttgttgcaga tggcgacggg catggcgtaa ctggcgcagc gtggtggagt aaatgccggt
    29521 gatcgcactg ttgcgcacca tggtgtagaa catacgcaag atggcttccg gctggcgcat
    29581 aaatagtgtt tcatcacgca ggtcgattag cgtaccgcgt agctgaaact catcgtcgag
    29641 tggacgtggt ttttcgtcgg cgggaagggc gaggatggct tcatcgaaca gttgcagcag
    29701 catctggttg agttcactga cgcggcgtgt aacgcggaag taatccttca tcatccgctc
    29761 gaccggttcg ttgccttcac cactgtaatt cagacgctga gcgacgctaa gttggcgatc
    29821 gaataacaga cgattatcgt aacggctgac gaccagatgc agggcaaagc gaatacgcca
    29881 cagtatatgc agacattcgt ttaattccgc ccgctccgct gaggttaaga agccaaaccc
    29941 gaccatttca tccagcgatg ttgcgccaaa atgacggcgg gccacccatt gcagagtgtg
    30001 aatatcgcgc aagccgccag ggctactttt gatgtctggt tcaaggttgt agctggtgcc
    30061 atggtaacgc tgatggcgct ggttctgttc ttcaactttc gctgcgtaga acttgtcgga
    30121 aggccagaat ccttcgctaa aaatatgttt ttgcagttcg aggaagagcg caacatcgcc
    30181 aattaataag cgggattcga ttaaattggt ggcgacggtt aaatccgata acccttccaa
    30241 catgcactct tcaagcgtgc gcacactatg accgacttcc agctttacat cccagagcag
    30301 cgttaacagc tcgcccactt tttgcgcctg atcgtccggg agctttttac ggcttaaaat
    30361 cagtaaatcg atgtcagaaa gtggatgcag ctcgccacga ccgtagccac cgacggcaac
    30421 caatgccagg tcggcaatct ggctgaatcc cgcttcaatc cataatcgtt gcaggagctg
    30481 gtctatgaac tcggtacgtg cctcaatcaa ctgttctgca gagatcccat tgtcaaaggc
    30541 atcacccagc caacgctgga aagtatcgat atgggctttt atcccacaga cggttaattc
    30601 atcacggggc caggcgcatg gattttgcgg ttgaccgggc agggtggaga gagcggtgtt
    30661 tgcgtactgt tctggaaggg tattcattgt gcgccaccca taagattaaa ttatcgcatt
    30721 aaaaaagccg gcattcgccg gcttcatctt attcgtcgtg cgagattatc gccgggatgg
    30781 tgtcatcctt gcgtagcgtc aaaatttcgc agccgttatc agtcaccaca atagtatgct
    30841 catattgtgc agacaagctg cgatctttgg tttttaccgt ccagccatct ttcatggtgc
    30901 ggatctcttt tttacccgcg ttgaccattg gctcaatggt gaacgtcatc cccggtttca
    30961 gcacgacgtt ggtttcacgg gagtcatagt gcagcacctg cggttcttca tggaagccgc
    31021 gaccaatacc gtgtccgcaa tattcacgaa cgacggagaa gccttctgct tcgacaaatt
    31081 tctgaatcgc cgcaccgatt tcgcgcagat taatgcctgg ttttaccatg cgtagcgcca
    31141 ggtacaggct ttcttgcgtg atgcggcaca ggcgttcgcc catgatggtc ggcttaccga
    31201 cgataaacat tttcgaggta tcgccgtgga aaccatcttt gattacggtg acatcaatgt
    31261 taacgatatc gccatctttc agcagcttag catcgtccgg gataccgtgg cacaccactt
    31321 cattaataga gatgcaaacg gatttcggat agccgtgata gccgaggcag gcagaaaccg
    31381 cgtgttgttc attaacaatg taatcattac agatgcgatc cagctcgccg gtgctgacgc
    31441 ccggtttaac atacggttcg atcatctcca gcacttcggc agccagtcgg ccagcgacgc
    31501 gcattttttc gatatcttct ggggtcttga ttgagatagc cattaattct gtccatcagc
    31561 gtcggtgata ccgacaatat atatgtaagt gccgtcaatg gtatcacacc cgggcaaatt
    31621 gagaatcatt ctgaatttcg ccaaacgtgc cactgaaggt tttctataat agaaaattcg
    31681 acgtctgatg ctgtacacag cgccaacaat tattggtgtc cacgacgtat ttgtggtata
    31741 aagcgcgccg gacttccgat ccatttcgta tacacagact ggacggaagc gacaatctca
    31801 ctttgtgtaa caacacacac gtatcggcac atattccggg gtgccctttg gggtcggtaa
    31861 tatgggatac gtggaggcat aaccccaact tttatataga ggttttaatc atggcaactg
    31921 tttccatgcg cgacatgctc aaggctggtg ttcacttcgg tcaccagacc cgttactgga
    31981 acccgaaaat gaagccgttc atcttcggtg cgcgtaacaa agttcacatc atcaaccttg
    32041 agaaaactgt accgatgttc aacgaagctc tggctgaact gaacaagatt gtttctcgca
    32101 aaggtaaaat ccttttcgtt ggtactaaac gcgctgcaag cgaagcggtg aaagacgctg
    32161 ctctgagctg cgaccagttc ttcgtgaacc atcgctggct gggcggtatg ctgactaact
    32221 ggaaaaccgt tcgtcagtcc atcaaacgtc tgaaagacct ggaaactcag tctcaggacg
    32281 gtactttcga caagctgacc aagaaagaag cgctgatgcg cactcgtgag ctggagaaac
    32341 tggaaaacag cctgggcggt atcaaagaca tgggcggtct gccggacgct ctgtttgtaa
    32401 tcgatgctga ccacgaacac attgctatca aagaagcaaa caacttgggt attccggtat
    32461 ttgctatcgt tgataccaac tctgatccgg acggtgttga cttcgttatc ccgggtaacg
    32521 acgacgcaat ccgtgctgtg accctgtacc tgggcgctgt tgctgcaacc gtacgtgaag
    32581 gccgttctca ggatctggct tcccaggcgg aagaaagctt cgtagaagct gagtaataag
    32641 gcttgataac tcccccaaaa tagttcgagt tgcagaaagg cggcaagctc gagaattccc
    32701 gggagcttac atcagtaagt gaccgggatg agcgagcgaa gacaacgcat ctgcggcgcg
    32761 aaatatgaag ggggagagcc cttatagacc aggtagtaca cgtttggtta gggggcctgc
    32821 atatggcccc ctttttcact tttatatctg tgcggtttaa tgccgggcag atcacatctc
    32881 cgaggatttt agaatggctg aaattaccgc atccctggta aaagagctgc gtgagcgtac
    32941 tggcgcaggc atgatggatt gcaaaaaagc actgactgaa gctaacggcg acatcgagct
    33001 ggcaatcgaa aacatgcgta agtccggtgc tattaaagca gcgaaaaaag caggcaacgt
    33061 tgctgctgac ggcgtgatca aaaccaaaat cgacggcaac tacggcatca ttctggaagt
    33121 taactgccag actgacttcg ttgcaaaaga cgctggtttc caggcgttcg cagacaaagt
    33181 tctggacgca gctgttgctg gcaaaatcac tgacgttgaa gttctgaaag cacagttcga
    33241 agaagaacgt gttgcgctgg tagcgaaaat tggtgaaaac atcaacattc gccgcgttgc
    33301 tgcgctggaa ggcgacgttc tgggttctta tcagcacggt gcgcgtatcg gcgttctggt
    33361 tgctgctaaa ggcgctgacg aagagctggt taaacacatc gctatgcacg ttgctgcaag
    33421 caagccagaa ttcatcaaac cagaagacgt atccgctgaa gtggtagaaa aagaatacca
    33481 ggtacagctg gatatcgcga tgcagtctgg taagccgaaa gaaatcgcag agaaaatggt
    33541 tgaaggccgc atgaagaaat tcaccggcga agtttctctg accggtcagc cgttcgttat
    33601 ggaaccaagc aaaactgttg gtcagctgct gaaagagcat aacgctgaag tgactggctt
    33661 catccgtttc gaagtgggtg aaggcatcga gaaagttgag actgactttg cagcagaagt
    33721 tgctgcgatg tccaagcagt cttaattatc aaaaaggagc cgcctgaggg cggcttcttt
    33781 ttgtgcccat cttgtaaatt cagctaaccc ttgtggggct gcgctgaaaa gcgacgtaca
    33841 atgtcgctag tattaattca tttcaatcgt tgacagtctc aggaaagaaa catggctacc
    33901 aatgcaaaac ccgtctataa acgcattctg cttaagttga gtggcgaagc tctgcagggc
    33961 actgaaggct tcggtattga tgcaagcata ctggatcgta tggctcagga aatcaaagaa
    34021 ctggttgaac tgggtattca ggttggtgtg gtgattggtg ggggtaacct gttccgtggc
    34081 gctggtctgg cgaaagcggg tatgaaccgc gttgtgggcg accacatggg gatgctggcg
    34141 accgtaatga acggcttggc aatgcgtgat gcactgcacc gcgcctatgt gaacgctcgt
    34201 ctgatgtccg ctattccatt gaatggcgtg tgcgacagct acagctgggc agaagctatc
    34261 agcctgttgc gcaacaaccg tgtggtgatc ctctccgccg gtacaggtaa cccgttcttt
    34321 accaccgact cagcagcttg cctgcgtggt atcgaaattg aagccgatgt ggtgctgaaa
    34381 gcaaccaaag ttgacggcgt gtttaccgct gatccggcga aagatccaac cgcaaccatg
    34441 tacgagcaac tgacttacag cgaagtgctg gaaaaagagc tgaaagtcat ggacctggcg
    34501 gccttcacgc tggctcgtga ccataaatta ccgattcgtg ttttcaatat gaacaaaccg
    34561 ggtgcgctgc gccgtgtggt aatgggtgaa aaagaaggga ctttaatcac ggaataattc
    34621 ccgtgatgga taaataaggg taagattccg cgtaagtatc gcgggggcgt aagtctggtt
    34681 ataaggcgtt attgttgcag gcagtttggt cacggccagc gcgcagcaac cggagcgtac
    34741 aaaagtacgt gaggatggcg agcactgctc ggggccaaaa tggcaaataa aatagcctaa
    34801 taatccagac gattacccgt aatatgttta atcagggcta tacttagcac acttccactg
    34861 tgtgtgactg tctggtctga ctgagacaag ttttcaagga ttcgtaacgt gattagcgat
    34921 atcagaaaag atgctgaagt acgcatggac aaatgcgtag aagcgttcaa aacccaaatc
    34981 agcaaaatac gcacgggtcg tgcttctccc agcctgctgg atggcattgt cgtggaatat
    35041 tacggcacgc cgacgccgct gcgtcagctg gcaagcgtaa cggtagaaga ttcccgtact
    35101 ctgaaaatca acgtgtttga tcgttcaatg tctccggccg ttgaaaaagc gattatggcc
    35161 tccgatctcg gcctgaaccc gaactctgcg ggtagcgaca tccgtgttcc gctgccgccg
    35221 ctgacggaag aacgtcgtaa agatctgacc aaaatcgttc gtggtgaagc agaacaagcg
    35281 cgtgttgcag tacgtaatgt gcgtcgtgac gcgaacgaca aagtgaaagc actgttgaaa
    35341 gataaagaga tcagcgaaga cgacgatcgc cgttctcagg acgatgtaca gaaactgact
    35401 gatgctgcaa tcaagaaaat tgaagcggcg ctggcagaca aagaagcaga actgatgcag
    35461 ttctgatttc ttgaacgaca aaaacgccgc tcagtagatc cttgcggatc ggctggcggc
    35521 gttttgcttt ttattctgtc tcaactctgg atgtttcatg aagcaactca ccattctggg
    35581 ctcgaccggc tcgattggtt gcagcacgct ggacgtggtg cgccataatc ccgaacactt
    35641 ccgcgtagtt gcgctggtgg caggcaaaaa tgtcactcgc atggtagaac agtgcctgga
    35701 attctctccc cgctatgccg taatggacga tgaagcgagt gcgaaacttc ttaaaacgat
    35761 gctacagcaa caggggagcc gcaccgaagt cttaagtggg caacaagccg cttgcgatat
    35821 ggcagcgctt gaggaggttg atcaggtgat ggcagccatt gttggtgctg ctgggctgtt
    35881 acctacgctt gctgcgatcc gcgcgggtaa aaccattttg ctggccaata aagaatcact
    35941 ggttacctgc ggacgtctgt ttatggacgc cgtaaagcag agcaaagcgc aattgttacc
    36001 ggtcgatagc gaacataacg ctatttttca gagtttaccg caacctatcc agcataatct
    36061 gggatacgct gaccttgagc aaaatggcgt ggtgtccatt ttacttaccg ggtctggtgg
    36121 ccctttccgt gagacgccat tgcgcgattt ggcaacaatg acgcccgatc aagcttgccg
    36181 tcatccgaac tggtcgatgg gacgtaaaat ttctgtcgat tcggctacca tgatgaacaa
    36241 aggtctggaa tacattgaag cgcgttggct gtttaacgcc agcgccagcc agatggaagt
    36301 gctgattcac ccgcagtcag tgattcactc aatggtgcgc tatcaggacg gcagtgttct
    36361 ggcgcagctg ggggaaccgg atatgcgtac gccaattgcc cacaccatgg catggccgaa
    36421 tcgcgtgaac tctggcgtga agccgctcga tttttgcaaa ctaagtgcgt tgacatttgc
    36481 cgcaccggat tatgagcgtt atccatgcct gaaactggcg atggaggcgt tcgaacaagg
    36541 ccaggcagcg acgacagcat tgaatgccgc aaacgaaatc accgttgctg cttttcttgc
    36601 gcaacaaatc cgctttacgg atatcgctgc gttgaattta tccgtactgg aaaaaatgga
    36661 tatgcgcgaa ccacaatgtg tggacgatgt gttatctgtt gatgcgaacg cgagtgaagt
    36721 cgccagaaaa gaggtgatgc gtctcgcaag ctgaggataa tccggctaca gagagtcgcg
    36781 ctatttgtta gcgtagggct tcagtgatat agtctgcgcc atctgatcgt aagtagttgg
    36841 ctttataagg tcaggtatgc cgtggtttta cacggctttt ttttgtatag gcttcagtat
    36901 tcctgagtac cgtaaaccct gtcagggaat aaaaaacgcg tgatgttgtc tgctactcaa
    36961 ccacttagcg aaaaattgcc agcgcatggc tgccgtcatg ttgcgatcat tatggacggc
    37021 aatggtcgct gggcaaaaaa gcaagggaag attcgtgcct ttgggcataa agccggggca
    37081 aaatccgtcc gccgggctgt ctcttttgcg gccaacaacg gtattgaggc gttaacgctg
    37141 tatgccttta gtagtgaaaa ctggaaccga ccagcgcagg aagtcagtgc gttaatggaa
    37201 ctgtttgtgt gggcgctcga tagcgaagta aaaagtctgc accgacataa cgtgcgtctg
    37261 cgtattattg gcgataccag tcgttttaac tcgcgtttgc aagaacgtat tcgtaaatct
    37321 gaagcgctaa cagccggaaa taccggtctg acgctgaata ttgcggcgaa ctacggtgga
    37381 cgttgggata tagtccaggg agtcaggcaa ctggctgaaa aggtgcagca aggaaacctg
    37441 caaccagatc agatagatga agagatgtta aaccagcatg tctgtatgca tgaactggcc
    37501 cctgtagatt tagtaattag gactggggga gagcatcgca ttagtaactt tttgctttgg
    37561 caaattgcct atgccgaact ttactttaca gatgttctct ggcccgattt cgatgaacaa
    37621 gactttgaag gggcgttaaa tgcctttgct aatcgagagc gtcgtttcgg cggcaccgag
    37681 cccggtgatg aaacagcctg atgggggtcg cttttgctga agtatcgcct gatatctgct
    37741 tttgtgttaa tacccgtcgt catcgcggcg ttgtttctgt tgccgccggt ggggttcgcc
    37801 attgtaacgc tggtggtctg catgctggca gcgtgggaat ggggacagct tagcggtttt
    37861 accactcgtt cgcagcgagt atggttggcg gtgttatgcg ggttattgtt ggcgctgatg
    37921 ctttttctgt tgccggaata tcaccgaaat attcatcaac cgctggttga aatctcactt
    37981 tgggcttcgt tgggttggtg gattgtcgcg ctattgctgg tgctgtttta cccaggttcc
    38041 gcagcaatct ggcgtaactc taaaacattg cgccttattt ttggcgtgct aaccattgtt
    38101 cccttcttct ggggcatgct ggcgttacgg gcctggcact atgacgagaa tcattacagt
    38161 ggcgcaatat ggctgctcta tgtcatgatc ctggtatggg gcgctgactc cggcgcatat
    38221 atgtttggca aattgtttgg taaacataag ctggcaccga aggtttctcc gggtaaaacc
    38281 tggcaaggct ttataggtgg actcgctact gcagcggtaa tctcatgggg ttatggcatg
    38341 tgggcgaatc tcgacgtcgc tcccgtcacc ttactcattt gctctattgt cgcagcgtta
    38401 gcctcagtgc tcggcgatct gaccgagagt atgtttaagc gtgaagcagg aattaaggac
    38461 agcggtcatt taattccagg acacggtggt attttagatc gtattgatag cctgacggct
    38521 gcggtaccgg tctttgcttg cttgttgtta ctggtattca ggacgcttta acggaaggta
    38581 atatgctgag ttttctctgg gatttggctt cgttcatcgt tgcactgggt gtacttatca
    38641 ccgtgcatga atttggtcat ttctgggttg cccggcgttg tggtgttcgc gttgagcgtt
    38701 tctcaatagg gtttggtaag gcgctctggc ggcgaactga taagctcggc accgaatatg
    38761 ttatcgccct gatcccgttg ggcggttatg tcaaaatgct ggatgagcgc gcagaaccgg
    38821 tcgttccgga actccgccac catgccttca ataataaatc tgtcggccaa cgagcggcga
    38881 ttattgccgc aggtccggtt gcaaacttca tttttgctat ctttgcctac tggctggttt
    38941 ttattattgg tgtgcctggc gtacgtccgg tggttggcga aatagcagcc aattcgatag
    39001 ctgcggaagc acaaattgca ccaggtacgg aactaaaagc cgtagatggt atcgaaacgc
    39061 ctgattggga tgccgtgcgt ttgcagttgg tcgataaaat tggcgatgaa agcaccacca
    39121 ttacagtagc gccatttgac agcgaccaac ggcgggatgt aaagctcgat ttacgtcact
    39181 gggcgtttga gcctgataaa gaagatccgg tatcttcgct ggggattcgt cctcgtgggc
    39241 cgcaaattga acctgtactg gaaaatgtgc agccaaactc ggcggcaagc aaggcaggtt
    39301 tgcaagcagg cgacaggatc gttaaagtcg atggtcagcc cttaacgcag tgggtgacct
    39361 ttgtgatgct tgtccgggat aacccgggta aatccttagc gttagaaatc gaaaggcagg
    39421 ggagtccctt gtctttgaca ttaatcccgg agagtaaacc gggtaatggt aaagcgattg
    39481 gttttgtcgg tattgagccg aaagtcattc ctttgccaga tgagtataaa gttgtacgcc
    39541 agtatgggcc gttcaacgcc atcgtcgaag ccacggacaa aacgtggcag ctgatgaagc
    39601 tgacggtcag tatgctggga aaattgatca ccggtgatgt gaaactgaac aacctcagtg
    39661 ggccgatctc tatcgccaag ggggctggga tgacagcgga actcggggta gtttattacc
    39721 tgccgtttct tgcgcttatt agcgtgaact tagggataat taacctgttt ccgttgcccg
    39781 tacttgacgg ggggcatctg ctgttccttg cgatcgaaaa gatcaagggc ggaccggtat
    39841 ccgagcgggt tcaagacttt tgttatcgca ttggctcgat tctgctggtg ctgttaatgg
    39901 ggcttgcact tttcaatgat ttctctcggt tatgagagtt agttaggaag aacgcataat
    39961 aacgatggcg atgaaaaagt tgctcatagc gtcgctgctg tttagcagcg ccaccgtata
    40021 cggtgctgaa gggttcgtag tgaaagatat tcatttcgaa ggccttcagc gtgtcgccgt
    40081 tggtgcggcc ctcctcagta tgccggtgcg tacaggcgac acggttaatg atgaagatat
    40141 cagtaatacc attcgcgctc tgtttgctac cggcaacttt gaggatgttc gcgtccttcg
    40201 tgatggtgat acccttctgg ttcaggtaaa agaacgtccg accattgcca gcattacttt
    40261 ctccggtaac aaatcggtga aagatgacat gctgaagcaa aacctcgagg cttctggtgt
    40321 gcgtgtgggc gaatccctcg atcgcaccac cattgccgat atcgagaaag gtctggaaga
    40381 cttctactac agcgtcggta aatatagcgc cagcgttaaa gctgtcgtga ccccgctgcc
    40441 gcgcaaccgt gttgacctaa aactggtgtt ccaggaaggt gtgtcagctg aaatccagca
    40501 aattaacatt gttggtaacc atgctttcac caccgacgaa ctgatctctc atttccaact
    40561 gcgtgacgaa gtgccgtggt ggaatgtggt aggcgatcgt aaataccaga aacagaaact
    40621 ggcgggcgat cttgaaaccc tgcgcagcta ctatctggac cgcggttatg cccgtttcaa
    40681 catcgactct acccaggtca gtctgacgcc agataaaaaa ggtatttacg tcacggtgaa
    40741 catcaccgaa ggcgatcagt acaagctttc tggcgttgaa gtgagcggca accttgccgg
    40801 gcactccgct gaaattgagc aactgactaa gatcgagccg ggcgagctgt ataacggcac
    40861 taaagtgacc aaaatggaag atgacattaa aaagcttctc ggtcgctatg gttatgccta
    40921 tccgcgcgta cagtcgatgc ctgaaattaa cgatgccgac aaaaccgtta aattacgcgt
    40981 gaacgttgat gcgggtaacc gtttctacgt gcgtaagatc cgttttgaag gtaacgatac
    41041 ctcgaaagat gccgtcctgc gtcgcgaaat gcgtcagatg gaaggtgcat ggttggggag
    41101 cgatctggtc gatcagggta aggagcgtct gaatcgtctg ggcttctttg aaactgtcga
    41161 taccgatacc caacgtgttc cgggtagccc ggaccaggtt gatgtagtct acaaggtgaa
    41221 agaacgtaac actggtagct tcaacttcgg gattggttac ggtactgaaa gtggcgtgag
    41281 cttccaggct ggcgtgcaac aggataactg gttaggtaca ggttatgctg ttggtatcaa
    41341 cgggaccaaa aacgattacc agacctatgc tgaactgtcg gtaaccaacc cgtacttcac
    41401 cgtagatggc gtaagcctcg gtggtcgtct cttctataat gacttccagg cagatgacgc
    41461 cgacctgtcc gactatacca acaagagtta tggtacagac gtgacgttgg gcttcccgat
    41521 taacgaatat aactcgctgc gtgcaggtct gggttatgta cataactccc tgtccaacat
    41581 gcagcctcag gttgcgatgt ggcgttatct gtactctatg ggtgaacatc cgagcacctc
    41641 tgatcaggat aacagcttca aaacggacga cttcacgttc aactatggtt ggacctataa
    41701 caagcttgac cgtggttact tcccgacaga tggttcacgt gtcaacctga ccggtaaagt
    41761 gaccattcct ggatcggata acgaatacta caaagtgacg ttagacacgg cgacttatgt
    41821 gccgatcgat gacgatcaca aatgggttgt tctgggtcgt acccgctggg gttatggtga
    41881 tggtttaggc ggcaaagaga tgccgttcta cgagaacttc tatgccggtg gttccagcac
    41941 cgtgcgtggc ttccagtcca ataccattgg tccgaaagca gtttacttcc cgcatcaggc
    42001 cagtaattat gatccggact atgattacga atgtgcgact caggacggcg cgaaagacct
    42061 gtgtaaatcg gatgatgctg taggcggtaa cgccatggcg gttgccagcc tcgagttcat
    42121 caccccgacg ccgtttatta gcgataagta tgctaactcg gttcgtactt cattcttctg
    42181 ggatatgggt accgtttggg atacaaactg ggattccagc caatattctg gatatccgga
    42241 ctatagtgat ccaagcaata tccgtatgtc tgcgggtatc gcattacaat ggatgtcccc
    42301 attggggccg ttggtgttct cctacgccca gccgttcaaa aagtacgatg gagacaaggc
    42361 agaacagttc cagtttaaca tcggtaaaac ctggtaagtg ttctccacaa aggaatgtag
    42421 tggtagtgta gcgatgactt taggcgatca atataagatc gccgggccac gcaaagaact
    42481 gcaccctccg gtgcaaatgg gatggtaagg agtttattgt gaaaaagtgg ttattagctg
    42541 caggtcttgg tttagcactg gcaacttctg ctcaggcggc tgacaaaatt gcaatcgtca
    42601 atatgggcag cctgttccag caggtagcgc agaaaaccgg tgtttctaac acgctggaaa
    42661 atgagttcaa aggccgtgcc agcgaactgc agcgtatgga aaccgatctg caggctaaaa
    42721 tgaaaaaact gcagtccatg aaagcgggca gcgatcgcac caaactggaa aaagacgtga
    42781 tggctcagcg ccagactttt gctcagaaag cgcaggcttt tgagcaggat cgcgcacgtc
    42841 gttccaacga agaacgcggc aaactggtta ctcgtatcca gactgctgtg aaatccgttg
    42901 ccaacagcca ggatatcgat ctggttgttg atgcaaacgc cgttgcttac aacagcagcg
    42961 atgtaaaaga catcactgcc gacgtactga aacaggttaa ataagtaatg ccttcaattc
    43021 gactggctga tttagcgcag cagttggatg cagaactaca cggtgatggc gatatcgtca
    43081 tcaccggcgt tgcgtccatg caatctgcac aaacaggtca cattacgttc atggttaacc
    43141 caaaataccg tgagcattta ggcttgtgcc aggcgtccgc ggttgtcatg acccaggacg
    43201 atcttccttt cgcgaaaagt gccgcgctgg tagtgaagaa tccctacctg acttacgcgc
    43261 gcatggcgca aattttagat accacgccgc agcccgcgca gaacattgca cccagtgcgg
    43321 tgatcgacgc gacggcgaag ctgggtaaca acgtatcgat tggcgctaac gcggtgattg
    43381 agtccggcgt tgaactgggc gataacgtga ttatcggtgc cggttgcttc gtaggtaaaa
    43441 acagcaaaat cggtgcaggt tcgcgtctct gggcgaacgt aaccatttac catgagatcc
    43501 agatcggtca gaattgcctg atccagtccg gaacagtggt aggcgcagac ggctttggtt
    43561 atgccaacga tcgtggtaac tgggtgaaga tcccacagat tggtcgcgta attattggcg
    43621 atcgcgtgga gatcggtgcc tgcacaacca tcgatcgcgg cgcgctggat gacactgtta
    43681 ttggcaatgg cgtgatcatt gataaccagt gccagattgc acataacgtc gtgattggcg
    43741 acaatacggc ggttgccggt ggcgtcatta tggcgggcag cctgaaaatt ggtcgttact
    43801 gcatgatcgg cggagccagc gtaatcaacg ggcatatgga aatatgcgac aaagtgacgg
    43861 ttacgggcat gggtatggtg atgcgtccca tcactgaacc aggcgtctat tcctcaggca
    43921 ttccgctgca acccaacaaa gtctggcgca aaaccgctgc actggtgatg aacattgatg
    43981 acatgagcaa gcgtctgaaa tcgcttgagc gcaaggttaa tcaacaagac taacgttcca
    44041 tcttttgttc gccaaacttt acggcctgtc tcattcttac gattgcggca ggccgtgtta
    44101 ttattgtcgt ttcttatatt ttgacaggaa gagtatcttg actactaaca ctcatactct
    44161 gcagattgaa gagattttag aacttctgcc gcaccgtttc ccgttcttac tggtggatcg
    44221 cgtgctggat tttgaagaag gtcgttttct gcgcgcagta aaaaatgtct ctgtcaatga
    44281 gccattcttc cagggccatt tccctggaaa accgattttc ccgggtgtgc tgattctgga
    44341 agcaatggca caggcaacag gtattctggc gtttaaaagc gtaggaaaac tggaaccggg
    44401 tgagctgtac tacttcgctg gtattgacga agcgcgcttc aagcgcccgg tcgtgcctgg
    44461 cgatcaaatg atcatggaag tcactttcga aaaaacgcgc cgcggcctga cccgttttaa
    44521 aggggttgct ctggtcgatg gtaaagtagt ttgcgaagca acgatgatgt gtgctcgtag
    44581 ccgggaggcc tgatacgtga ttgataaatc cgcctttgtg catccaaccg ccattgtgga
    44641 agagggcgcg tcaattggcg cgaacgcaca cattggtcct ttttgtatcg ttggacccca
    44701 tgtcgaaatt ggtgagggta ccgtactgaa atctcacgtt gtcgtgaatg gtcatactaa
    44761 aattggccgc gataatgaga tttatcagtt tgcctccatc ggcgaagtta accaggattt
    44821 gaaatatgct ggcgaaccga cccgtgtgga aatcggcgat cgtaaccgca ttcgcgaaag
    44881 cgtcaccatt catcgtggca cagtccaggg cggtggattg acgaaggtgg gcagcgacaa
    44941 cttactgatg atcaacgcgc acattgcgca cgattgtacg gtaggtaacc gctgtattct
    45001 cgccaacaac gcaacgctgg cgggtcacgt atcggttgac gacttcgcga tcatcggcgg
    45061 catgaccgca gtccatcagt tctgcatcat tggtgcgcat gtgatggttg gcggctgctc
    45121 cggtgtggcg caggacgtcc ctccttatgt tattgcgcag ggtaaccacg caacgccgtt
    45181 cggtgtcaat atcgaagggc tgaagcgccg cggattcagc cgtgaggcga ttaccgctat
    45241 ccgcaatgcg tataagctaa tttatcgtag cggtaaaacg ctcgatgaag tgaaaccgga
    45301 aattgctgaa ctggcggaaa catatccgga agtgaaagcc tttaccgatt tctttgcacg
    45361 ctcaacgcgc ggtctgattc gttaatgact gaccagcgtc cattaacgat tgccctggtc
    45421 gccggagaaa cctccggcga tatcctgggg gccggtttaa tccgcgctct gaaagaacgt
    45481 gtgcccaacg cccgctttgt tggtgttgcc gggccacgaa tgcaggctga aggctgcgaa
    45541 gcctggtacg aaatggaaga actggcggtg atgggcattg ttgaagtgct cggtcgtctg
    45601 cgtcgcttac tgcatattcg tgccgatctg acaaagcgtt ttggcgaact gaagccagat
    45661 gtttttgttg gtattgatgc gcccgacttc aatattactc ttgaaggtaa cctcaaaaag
    45721 cagggtatca aaaccattca ttacgtcagt ccatccgtct gggcgtggcg acagaaacgc
    45781 gttttcaaaa taggcagagc caccgatctg gtgctcgcat ttctgccttt cgaaaaagcg
    45841 ttttatgaca aatacaatgt accgtgccgc tttatcggtc ataccatggc tgatgccatg
    45901 ccgttagatc cagataaaaa tggtgcccgt gatgtgctgg ggatccctta cgatgcccac
    45961 tgtctggcat tgttgccggg cagccgtggt gcagaagtcg aaatgcttag tgccgatttc
    46021 ttgaaaactg cccagctttt gcgccagaca tatccggatc tcgaaatcgt ggtgccgctg
    46081 gtgaatgcca aacgccgcga gcagtttgaa cgcatcaaag ctgaagtcgc gccagacctg
    46141 tcggttcatt tgctggatgg aatgggccgt gaggcgatgg tcgccagcga tgcggcacta
    46201 ctggcgtcgg ggacggcagc cctggagtgt atgctggcga aatgcccgat ggtggtggga
    46261 tatcgcatga agccttttac cttctggttg gcgaagcggc tggtgaaaac tgattatgtc
    46321 tcgctgccaa atctgctggc gggcagagag ttagtcaaag agttattgca ggaagagtgt
    46381 gagccgcaaa aactggctgc ggcgctgtta ccgctgttgg cgaacgggaa aaccagccac
    46441 gcgatgcacg ataccttccg tgaactgcat cagcagatcc gctgcaatgc cgatgagcag
    46501 gcggcacaag ccgttctgga gttagcacaa tgatcgaatt tgtttatccg cacacgcagc
    46561 tggttgcggg tgtggatgaa gtcggacgcg ggccgttagt tggcgcggtc gtcaccgctg
    46621 cggtgatcct tgacccggcg cgcccgattg ccgggctgaa tgattccaaa aagctgagcg
    46681 aaaaacgccg tctggcgctc tatgaagaga tcaaagagaa agcgttgagc tggagtctgg
    46741 gccgcgcgga accccacgaa atcgacgagc tgaacattct tcatgcgacc atgctggcga
    46801 tgcagcgtgc cgtcgctggg ctgcatattg cgccggaata tgtgttgatt gatggtaacc
    46861 gctgcccgaa attaccgatg cctgcgatgg ctgtggtgaa aggggatagc cgcgtaccag
    46921 aaatcagtgc cgcgtctatc ctggcgaaag tgacgcgtga cgccgaaatg gcggcgctgg
    46981 atattgtttt cccgcaatat ggttttgccc aacataaagg gtatccaacc gcttttcatc
    47041 tggaaaaact ggctgaatat ggcgcgactg aacatcatcg tcgcagcttt gggcctgtca
    47101 aacgcgcact gggacttgcg tcctgattct tgtgtcgaga ttaagtaaac cggaatctga
    47161 agatgtctga accacgtttc gtacacctgc gggtgcacag cgactactcg atgatcgatg
    47221 gcttggccaa aaccgcaccg ctggtaaaaa aggcggcggc gttgggtatg ccagcactgg
    47281 cgatcaccga tttcaccaac ctttgcggtc tggtgaagtt ctacggagcg ggacatggcg
    47341 cagggattaa gcctatcgtc ggggcagatt ttaatgtcca gtgcgacctg ctgggtgatg
    47401 agttaaccca cctgacggta ctggcggcga acaataccgg ctatcagaat ctggcgttgc
    47461 tgatctcaaa agcgtatcag cgcgggtacg gtgccgccgg gccgatcatc gatcgcgact
    47521 ggcttatcga attaaacgaa gggttgatcc ttctttccgg cgggcgcatg ggcgacgtcg
    47581 gacgcagtct tttgcgtggt aacagcgcgc tggtagatga gtgtgtcgcg ttttatgaag
    47641 aacacttccc ggatcgctat tttctcgagc tgatccgcac cggcaggccg gatgaagaaa
    47701 gctatctgca cgcggcggtg gaactggcgg aagcgcgcgg tttgcccgtc gtggcgacta
    47761 acgatgtgcg ctttatcgac agcagcgact ttgacgcaca cgaaatccgc gtcgcgatcc
    47821 acgacggctt taccctcgac gatcctaaac gcccgcgtaa ctattcgccg cagcaatata
    47881 tgcgtagcga agaggagatg tgtgagctgt ttgccgacat cccagaagcc cttgccaaca
    47941 ccgttgagat cgccaaacgc tgtaacgtaa ctgtgcgtct tggtgaatac ttcctgccgc
    48001 agttcccgac cggggacatg agcaccgaag attatctggt caagcgtgca aaagaaggcc
    48061 tggaagagcg tctggcattt ttattccctg acgaggaaga acgtcttaaa cgccgcccgg
    48121 aatatgacga acgtctggag actgaacttc aggttatcaa ccagatgggc ttcccgggct
    48181 acttcctcat cgttatggaa tttatccagt ggtcgaaaga taacggcgta ccggtagggc
    48241 caggccgtgg ctccggtgcg ggttcactgg tggcctacgc gctgaaaatc accgacctcg
    48301 atccgctgga atttgacctg ctgttcgaac gtttccttaa cccggaacgt gtctccatgc
    48361 ctgacttcga cgttgacttc tgtatggaga aacgcgatca ggttatcgaa cacgtggcgg
    48421 acatgtacgg tcgtgatgcg gtatcgcaga ttatcacctt cggtacgatg gcggcgaaag
    48481 cggtgatccg cgacgtaggc cgcgtgctgg ggcatccgta cggctttgtc gaccgtatct
    48541 cgaaactgat cccgcccgat ccggggatga cgctggcgaa agcgtttgaa gccgagccgc
    48601 agctgccgga aatctacgaa gcggatgaag aagttaaggc gctgatcgac atggcgcgca
    48661 aactggaagg ggtcacccgt aacgccggta agcacgccgg tggggtggtt atcgcgccga
    48721 ccaaaattac cgattttgcg ccgctttatt gcgatgaaga gggcaaacat ccggtcaccc
    48781 agtttgataa aagcgacgtt gaatacgccg gactggtgaa gttcgacttc cttggtttgc
    48841 gtacgctcac catcatcaac tgggcgctgg agatgatcaa caagcggcgg gcgaagaatg
    48901 gcgagccacc gctggatatc gccgcgatcc cgctggatga caagaaaagc ttcgacatgc
    48961 tgcaacgctc ggaaaccacg gcggtattcc agcttgaatc gcgcggcatg aaggacctga
    49021 tcaagcgtct gcaacctgac tgcttcgaag atatgatcgc actggtggca ctgttccgcc
    49081 ctggtccgtt gcagtcaggg atggtggata actttatcga ccgtaaacat ggtcgtgaag
    49141 agatctccta tccggacgtg cagtggcagc atgaaagcct gaaatcggta ctggagccaa
    49201 cctacggcat tatcttgtat caggaacagg tcatgcagat tgcccaggtg ctttctggtt
    49261 ataccctcgg tggcgcggat atgctgcgcc gtgcgatggg taagaaaaag ccggaagaga
    49321 tggctaagca gcgttctgta tttgctgaag gtgcagaaaa gaacggaatc aacgccgaac
    49381 tggcgatgaa aatcttcgac ctggtggaga aattcgcggg ttacggattt aacaaatcgc
    49441 actctgcggc ctatgctttg gtgtcatatc aaacgttatg gctgaaagcg cactatccgg
    49501 cggagtttat ggcggcggta atgaccgccg atatggacaa caccgagaag gtggtgggcc
    49561 tggtggatga gtgctggcgg atggggctga aaatcctgcc accagatata aactccggtc
    49621 tttaccattt ccacgtcaac gacgatggcg aaatcgtgta tggtattggc gccatcaaag
    49681 gggtaggtga aggtccgatt gaggccatca tcgaagcccg taataaaggc ggctacttcc
    49741 gcgaactgtt tgatctctgc gcccgtaccg acaccaaaaa gttaaaccgg cgggtgctgg
    49801 aaaaactgat catgtccggg gcgtttgacc gtcttgggcc acaccgcgcg gcgctgatga
    49861 actcgctggg tgatgcgtta aaagcggcag atcaacacgc gaaagcggaa gctatcggtc
    49921 aggccgatat gttcggcgtg ctggccgaag agccggaaca aattgaacaa tcctacgcca
    49981 gctgccaacc gtggccggag caggtggtat tagatgggga acgtgaaacg ttaggcctgt
    50041 acctgaccgg acaccctatc aaccagtatt taaaagagat tgagcgttat gtcggaggcg
    50101 taaggctgaa agacatgcac ccgacagaac gtggtaaagt catcacggct gcggggctcg
    50161 ttgttgccgc gcgggttatg gtcaccaagc gcggcaatcg tatcggtatc tgcacgctgg
    50221 atgaccgttc cgggcggctg gaagtgatgt tgtttactga cgccctggat aaataccagc
    50281 aattgctgga aaaagaccgc atacttatcg tcagcggaca ggtcagcttt gatgacttca
    50341 gcggtgggct taaaatgacc gctcgcgaag tgatggatat tgacgaagcc cgggaaaaat
    50401 atgctcgcgg gcttgctatc tcgctgacgg acaggcaaat tgatgaccag cttttaaacc
    50461 gactccgtca gtctctggaa ccccaccgct ctgggacaat tccagtacat ctctactatc
    50521 agagggcgga tgcacgcgcg cggttgcgtt ttggcgcgac gtggcgtgtc tctccgagcg
    50581 atcgtttatt aaacgatctc cgtggcctca ttggttcgga gcaggtggaa ctggagtttg
    50641 actaatacag gaatactatg agtctgaatt tccttgattt tgaacagccg attgcagagc
    50701 tggaagcgaa aatcgattct ctgactgcgg ttagccgtca ggatgagaaa ctggatatta
    50761 acatcgatga agaagtgcat cgtctgcgtg aaaaaagcgt agaactgacg cgtaaaatct
    50821 tcgccgatct cggtgcatgg cagattgcgc aactggcacg ccatccgcag cgtccttata
    50881 ccctggatta cgttcgcctg gcatttgatg aatttgacga actggctggc gaccgtgcgt
    50941 atgcagacga taaagctatc gtcggtggta tcgcccgtct cgatggtcgt ccggtgatga
    51001 tcattggtca tcaaaaaggt cgtgaaacca aagaaaaaat tcgccgtaac tttggtatgc
    51061 cagcgccaga aggttaccgc aaagcactgc gtctgatgca aatggctgaa cgctttaaga
    51121 tgcctatcat cacctttatc gacaccccgg gggcttatcc tggcgtgggc gcagaagagc
    51181 gtggtcagtc tgaagccatt gcacgcaacc tgcgtgaaat gtctcgcctt ggcgtaccgg
    51241 tagtttgtac ggttatcggt gaaggtggtt ctggcggtgc gctggcgatt ggcgtgggcg
    51301 ataaagtgaa tatgctgcaa tacagcacct attccgttat ctcgccggaa ggttgtgcgt
    51361 ccattctgtg gaagagcgcc gacaaagcgc cgctggcggc tgaagcgatg ggtatcatcg
    51421 ctccgcgtct gaaagagctg aaactgatcg actccatcat cccggaaccg ctgggcggcg
    51481 ctcaccgtaa cccggaagcg atggcggcat cgttgaaagc gcaactgctg gcggatctgg
    51541 ccgatctcga tgtgttaagc actgaagatt taaaaaatcg tcgttatcag cgcctgatga
    51601 gctacggtta cgcgtaattc gcaaaagttc tgaaaaaggg tcacttcggt ggcccttttt
    51661 tatcgccacg gtttgagcag gctatgatta aggaaggatt ttccaggagg aacacatgaa
    51721 catcattgcc attatgggac cgcatggcgt cttttataaa gatgagccca tcaaagaact
    51781 ggagtcggcg ctggtggcgc aaggctttca gattatctgg ccacaaaaca gcgttgattt
    51841 gctgaaattt atcgaacata accctcgaat ttgcggcgtg atttttgact gggatgagta
    51901 cagtctcgat ttatgcagcg atatcaatca acttaatgaa tatctcccgc tttatgcctt
    51961 catcaacacc cactcgacga tggatgtcag cgtgcaggat atgcggatgg cgctctggtt
    52021 ttttgaatat gcgctggggc aggcggaaga tatcgccatt cgtatgcgtc agtacaccaa
    52081 cgaatatctt gataacatta cgccgccgtt cacgaaagcc ttgtttacct acgtcaaaga
    52141 gcggaagtac accttttgta cgccggggca tatgggcggc accgcatatc aaaaaagccc
    52201 ggttggctgt ctgttttatg attttttcgg cgggaatacc cttaaggctg atgtctctat
    52261 ttcggtcacc gagcttggtt cgttgcttga ccacaccggg ccacacctgg aagcggaaga
    52321 gtacatcgcg cggacttttg gcgcggaaca gagttatatc gttaccaacg gaatatcgac
    52381 gtcgaacaaa attgtgggta tgtacgccgc gccatccggc agtacgctgt tgatcgaccg
    52441 caactgccat aaatcactgg cgcatctgtt gatgatgaac gatgtagtgc cagtctggct
    52501 gaaaccgacg cgtaatgcgt tggggattct gggggggatc ccgcgccgtg aatttactcg
    52561 cgacagcatc gaagagaaag tcgctgccac tacgcaagca caatggccgg ttcatgcggt
    52621 gatcaccaac tccacctatg atggtttgct ctacaacacc gactggatca aacagacgct
    52681 ggatgtcccg tcgatccact tcgattccgc ctgggtgccg tatactcatt ttcatccaat
    52741 ctaccagggt aaaagtggta tgagcggcga gcgtgttgcg ggaaaagttt tcttcgaaac
    52801 gcaatcgacc cacaaaatgc tggcggcgtt atcgcaggct tcactgatcc acattaaagg
    52861 cgagtatgac gaagaggcgt ttaacgaagc ctttatgatg cataccacca cctcgcccag
    52921 ttatcccatt gttgcttcgg ttaagacggc ggcggcgatg ctgcgtggta atccgggcaa
    52981 acggctgatt aaccgttcgg tggagcgggc gctgcatttt cgcaaagagg tccagcggct
    53041 gcgggaagag tctgacggtt ggtttttcga tatctggcaa ccgccgcagg tggatgaagc
    53101 cgaatgcagg cccgttgcgc ctggcgaaca atggcacggc tttaacgatg cggatgccga
    53161 tcatatgttt ctcgatccgg ttaaagtcac tattttgaca ccggggatgg acgaacaggg
    53221 caatatgagt gaggagggga tcccggcggc gctggtagca aaattcctcg acgaacgtgg
    53281 gatcgtagta gagaaaaccg gcccttataa cctgctgttt ctctttagta ttggcatcga
    53341 taaaaccaaa gcaatgggat tattgcgtgg gttgacggaa tttaagcgct cttacgatct
    53401 caacctgcgg atcaaaaata tgctgcccga tctctatgct gaagatcccg atttctaccg
    53461 caatatgcgt attcaggatc tggcgcaagg gatccataag ctgattcgta aacacgatct
    53521 ttccggtttg atgttgcggg cattcgatac tttgccggaa atgatcatga cgccacatca
    53581 ggcatggcag agacagatta aaggtgaagt agaaaccatt gcgccggaac aactggtcgg
    53641 tagagtatcg gcaaatatga tcctgcctta tccaccgggc gtaccgctgt tgatgccagg
    53701 agaaatgctg accaaagaga gccgcacggt actcgatttt ctactgatgc tttgttccgt
    53761 agggcaacat taccccggtt ttgaaacgga tattcacggc gcgaaacagg acgaagacgg
    53821 cgtttaccgc gtacgagtcc taaaaatggc gggataactt gccagagcgg cttccgggcg
    53881 agtaacgtgc tgttaacaaa taaaggagac gttatgctgg gtttaaaaca ggttcaccat
    53941 attgcgatta ttgcgacgga ttatgcggtg agcaaagctt tctactgcga tattcttggt
    54001 ttcacgctgc aaagcgaagt ctatcgcgaa gcgcgcgact cgtggaaagg ggatttggcg
    54061 cttaatgggc aatatgtgat tgagcttttc tcttttccgt tcccgccgga gcgccccagc
    54121 cgaccggaag cttgcggcct gcgtcatctg gcttttagcg ttgatgacat cgatgcggca
    54181 gtggcgcacc ttgaaagcca taacgtgaag tgtgaagcca tccgtgtcga tccatacacg
    54241 caaaaacgct tcaccttctt taacgatccg gacgggctgc cgttggaact gtatgagcag
    54301 taaggcttgt catcgccgca tttgcccggt aacgtgccgg gcattgctac tgtaaaatcg
    54361 caccatcatg acactcacgc tcaatagaca acttctcacc tcacgccaga ttctggtggc
    54421 ctttagcggc gggcttgact ccaccgttct gctgcatcag ttggtgcagt ggcggacgga
    54481 aaatccgggc gtcactctgc gcgctattca tgtgcatcac ggtttaagtg ccaatgccga
    54541 tgcctgggtt acgcattgcg aaaacgtctg ccaacagtgg caggtgccgc tggtggtcga
    54601 acgcgtacaa cttgcgcaag aggggctggg gattgaggcc caggcgcggc aggcacgtta
    54661 tcaggcattt gcccgtaccc tattgcccgg cgaagtgctg gtcaccgcgc aacatctcga
    54721 cgatcaatgt gaaacctttc tgctggcgct aaaacgcggt agcggccctg ccgggctttc
    54781 ggctatggcg gaagtttcgg agtttgctgg aacacggctt atccgcccgt tgcttgcccg
    54841 cacgcggggg gaactggagc agtgggcatt ggcgcatggt ttacgctgga ttgaagacga
    54901 aagtaatcag gacgactcat acgatcgtaa ctttctgcgc ctgcgcgtag tgccgttatt
    54961 gcagcagcgt tggccgcatt ttgcggaagc aacggcccgc agcgccacac tttgtgctga
    55021 acaagagagt ctgttggatg aactgctggc ggatgattta gcacactgcc aaacgtcgca
    55081 ggggacgctg cagattgcgc caatgctggc gatgagtgat gcccgtcgcg cggcgattat
    55141 tcgccgctgg ctggcagggc agaatgcacc gatgccttcc cgcgacgcgc tggtgcgcat
    55201 ctggcaggaa gtggcgctgg cgcgggaaga tgcttccccc tgtttacgtt tgggcgcctt
    55261 tgaaatccgc cgctatcagt cgcaactgtg gtggattaaa tccgtcaccg ggcaaagcga
    55321 aaccattgtg ctgtggcaga cgtggctaca accgctggaa ttaccggcgg ggctgggaac
    55381 cgtacagctt actgcgggag gcgatattcg ccctccgcgt gcagacgaag cggtcagcgt
    55441 gcgtttcaaa gcgccaggat tgctgcatat tgtcgggcgc aacggcggac gtaagctgaa
    55501 gaaaatctgg caagagttag gcgtgccgcc gtggctacgt gacaccacgc cactgctgtt
    55561 ttatggagaa acgctgattg cggcggcagg ggtatttgtg acgcaagaag gtgtggctga
    55621 aggtgagaat ggcgtaagtt ttgtctggca gaaaacgctt agttaagtga aagccggata
    55681 agacgcatca aacgtcgcat ccggcgaaag tcaatcagga ttcgcttacc accaccgtac
    55741 cgatttcagg gtggctaaag ctggtaattt tatccagacg cagctcgcgg gtttcgccag
    55801 cggcctcgac gaccagatac tccacatttt tgcgggagac taaatcactg gcttttgcct
    55861 gcaatttttc gccatctttc agctcaagtg tcagcattaa atgatgctgg caggcgagct
    55921 caagattatc gtaatcatca caattaattg gttgatacgt atcattcatt gacataatcg
    55981 ctcaccagta agtttgccgc agcgtatgct gctttttccc tgacagcctc agaaagggcg
    56041 tcgtcggcag ccatttcatt cagcactttc aaaacgcagc ccagcgcgtc cggaacgtat
    56101 cctaagtctc cgctggcgat ttccgcgtac cgcttgcgta ttaactcaca atatttttcc
    56161 acatgccctc ctgtcagcac tctgacttaa ccgtggatgc aagtctaagc ctacgaagat
    56221 aaactctgtt tcgcaaggtg actataccac actcatttct gcaatatcag cgccgcaact
    56281 gcacgtattc cgttacaatg gcctcctgat tcgaaaggag ttttcttatg gcgcttaaag
    56341 cgacaattta taaagcgacg gttaatgtgg ccgatctcga ccgcaaccag tttctcgatg
    56401 cctctctgac gctggcgcgc cacccttcag aaacccagga gcgtatgatg ctgcgcttgc
    56461 tggcgtggct gaaatatgct gatgaacgtc tgcaatttac ccgtggtttg tgtgccgatg
    56521 atgagccgga agcgtggctg cgtaacgatc acctgggcat tgatttgtgg attgagctgg
    56581 ggctgccgga tgagcgacga attaagaaag cctgcaccca ggccgcagaa gtggcgctgt
    56641 ttacctataa tagtcgggcg gcgcaaatct ggtggcagca aaatcagagc aaatgtgtgc
    56701 agtttgccaa tctttccgtc tggtatctgg acgatgaaca actggcgaaa gtaagcgcct
    56761 ttgccgatcg taccatgacg ctgcaggcaa cgattcagga tggtgttatc tggttatcgg
    56821 atgataagaa taatctggaa gttaacttaa ccgcctggca acaaccttca tgattgtgat
    56881 ttcccgacat gttgctattc ccgatggtga gcttgagatc accgccattc gtgcgcaggg
    56941 cgctggcggg cagcatgtta ataagacctc aacggctatt catctgcgtt ttgacattcg
    57001 ggcgtccagc ctgccagagt attacaaaga gcgtctgctc gccgccagcc atcatttgat
    57061 cagcagtgat ggcgtgattg tcattaaggc acaggaatac cgcagtcagg aactgaaccg
    57121 cgaagccgct ctggcccggc tggtggcagt gattaaagat ttaacaaaag aacaaaaagc
    57181 ccgacgaccc acgcggccca cccgtgcatc gaaagagcgc aggctggcat cgaaagcaca
    57241 aaaatcaagc gtgaaggcga tgcgcggcaa agtacgcagc ggtcgggaat aaaaagaagg
    57301 aatggatggt gaaaaaagcg atagtgacag cgatggctgt aatcagcctc tttactctga
    57361 tgggatgtaa taatcgggcc gaagtcgata cgctttctcc ggcgcaggct gccgaactga
    57421 aaccgatgcc gcaaagttgg cgcggcgtgc taccgtgtgc cgattgcgaa ggaatcgaaa
    57481 tctctctgtt cctcgaaaaa gacggaacat gggtgatgaa tgagcgttat ctcggtgctc
    57541 gtgaagaacc ttcctccttc gcttcctacg gtacatgggc gcgaaccgct gacaagctgg
    57601 tattaaccga cagcaaaggt gaaaagtcat attatcgggc gaaaggcgat gcgctggaga
    57661 tgctcgatcg tgaaggcaat ccaattgaat cgcagttcaa ctatacgctg gaaccggcac
    57721 aatccagttt acctatgacg ccgatgaccc tgcgaggcat gtatttttat atggctgatg
    57781 cggcgacctt cactgattgc gcgaccggaa aacgtttcat ggtagcgaat aacgcagagc
    57841 tggagcgtag ctacctggct gcgcgcggtc acagtgaaaa accggtgtta ctgtcagtag
    57901 aaggtcactt tacgcttgag gctaatccgg atactggtgc gccaaccaaa gtattagcgc
    57961 ccgatacggc gggtaaattt tacccgaata aggattgcag tagtttgggg cagtaaacct
    58021 gcttaaggca gaaacaaacg caaaattgcc tgatgcgctg cgcttatcag gcctacagca
    58081 tataatgcaa cattttgaat tagcacgatt ttgtaggccg gataaggcgt ttacgccgca
    58141 tccggcattt tgtgcacgag gctggcttta tcactgagtc agcccaacaa agcgacccgc
    58201 cttgatataa atcccaggct tcaggtgccc aacatactgt aactgcgttt ctggcttaaa
    58261 cgcagagaca tcaccaatac gcatatgcat cagatcggca gggctaatcc agcccgattg
    58321 cgccagtgta agtggatgcc cggatttggc aaatgcatca gtgacaaatt ccgaacaaaa
    58381 ccacgacttt ttgtctcctt cgcacacact gcttaactgc gctttcgcca ggtcgctgac
    58441 gcactgttgg cgaaaatcct cggagaacgg attcagtgag cacatctggc gagtcaccat
    58501 aaagggaata aattcgacaa tgccgcgata gttataaccg ctatctttga ttttattggc
    58561 aaacgcggtg atatctgtgg cttgttgcgg ggtaagatcc gggactcgta agacgaaaag
    58621 cttatcacta tgcttcatcg cttttttaag ggaaacaatc tggacgccag cgcctgtcgc
    58681 ttctgcaacg ttattatcac ccagaaagat tgcaacgtga ctcacagagg aagtgctgaa
    58741 gacgcggatt ccaaatgagg ttaccccaag gcttgaggag aacagcaaat cgccgggttt
    58801 gagatcaggt gccgttattt cttttattga ttgttcggtg aaagagcttt gatgctggaa
    58861 tttgacagcc catgtttttg cctcggcatc tactgctgtg gctgatgggt ctggctggct
    58921 gatatcaacg gtacaagccg aaagtaaaag aaagctgggg agaagcaggc ggcagtacgc
    58981 ctttggttta tccattttat acaatccatg taaaaaaggg ccctgaaatt caggaccctt
    59041 tctggcatca gcctttaatc tgtttcacca gatattcgac gatgtcacca gtcttaatta
    59101 actgtttctc gccgttgcga cgatatttat attcgatatc gtcgttgtcg aggttacggt
    59161 cgcccagcac aatagtgtgc ggaataccga tcagttccat atcagcaaac atcacgcccg
    59221 gacgctcttt gcggtcatcc agcagcactt cgataccttg tgcacgcagt tcgctgtaca
    59281 gtttctcagc aagctcttgt acgcggaagg atttgtgcat gttcatcggc agaatcgcca
    59341 cctggaacgg cgcgatagcg tcaggccata cgatgccgcg ttcgtcgtag ttctgctcaa
    59401 tcgccgcagc taccacacgc gttaccccga taccgtagca acccatcgtc aggatttggt
    59461 tacggccatc ttcaccctgt acggaggctt tcagtgcttc ggagtacttg gtacccagct
    59521 ggaagatgtg accaacttcg ataccacgtt tgatcagcag cgtaccctgg ccatccgggc
    59581 ttggatcgcc agccaccacg ttacggatat ctgcaacttc cggggtagcg acatcgcgat
    59641 cccagttgat accgaagtag tgtttaccat cgatgttagc accagcagcg aaatcactca
    59701 tcgccgcaac ggtacggtca atcaccaccg gaatcggcat gtttaccgga cccagtgaac
    59761 ccggaccggc tttaaccaca gcacgaattt cttcttcggt cgcgaaagtc agcgggcttg
    59821 caacctgcgg cagtttttct gctttaactt cgttcagctc gtgatcaccg cgcaccagca
    59881 gcgcaaccag cgggaagcta ctgccttcaa ccgctttaac cagcagagtc ttaaccgttt
    59941 tctcaatcgg cagattgaac tgttcaacca gttccgcgat ggttttcgcg ttcggcgtat
    60001 caaccagcgt catttcctgg gtagcagcag cgcgcggttc tttcggcgcg atagcttctg
    60061 ccagttcaat gttcgctgca tagtcagagg tgtcggagaa gaccacatcg tcttcaccgc
    60121 tctgcgccag cacctggaat tcgtgagagg cgctgccgcc gatagaaccg gtgtcggcct
    60181 gtacggcgcg gaaatccagc cccatgcggc tgaagatttt gctgtaggcc gcatacattg
    60241 catcgtaggt ttcctgcagg gattcctgag aagtatggaa agagtaagca tctttcatca
    60301 ggaattcgcg ggaacgcatg acgccgaaac gcggacgcac ttcgtcgcgg aacttggtct
    60361 ggatctgata gaagttcagc ggcagctgtt tgtaagagct aagctcgtta cgaatcaggt
    60421 cagtgataac ttcttcatga gttgggccga gtacgaacgg acgctcgcca cggtcaacaa
    60481 aacgcagcag ttccggaccg tactgttccc aacgaccact ctcttgccac aaatcggctg
    60541 gctgaaccac cggcatcgac acctcgatcg caccggcgtt gttcatctct tcacgcacga
    60601 tgttttcgac ttttttcaga acgcgcacgc cggtcggcag ccaggtatat aacccggagg
    60661 ccagcttgcg gatcatcccg gcgcgcagca tcagctgatg gctgatcacc tcggcgtcgg
    60721 caggtgtctc cttgagagtg gagagcaggt attggctagt acgcatgttg ttacggttcc
    60781 agttggaagg tagaacaggc tcaaggcgag cctgggacaa aaaaagtgat ttagtttacc
    60841 agtgcaaaag aaatgtcaaa agagaagggc gtgaatttaa cgcggttcca gcgcaaagac
    60901 ttcaaaacct gcgtcggtga cgcgccagcg aacgttaaaa tcatgtagcc agacggcata
    60961 ggttttgccc gtttcctcac ctttacgata ggccgggcgc gggtcctgcg ccagtacttc
    61021 gcggataaac agcgttaact gcggataacg cttctccagc gtcaaaagct gcttttcgac
    61081 ctctgcggta aaactcaccg ccatctctgc agctggcgca ctttgcgcat agctggcact
    61141 ggcatcggga agcgattcgg caaaggggag atacggtttg atatccacta ccggcgtacc
    61201 atcgaccaga tccagactgc cgagcttcag aatcacgctg cctttatggc aaacaacctc
    61261 tttcagctct accagcgaca tgccaattgg gttagggcgg aaagtagagc gtgttgcgaa
    61321 aacccccatt ctggcgttac cgccgaggcg cggtggacgc acagtcggac gccagccgcc
    61381 ttccatcgtt tgatgaaaga cgaaaaggat ccataaatgg ctgaatgctt ccaggccgcg
    61441 aacggcgtcg gcctggttgt aggaagctat gagatgcagt tcgccgttgg cgctttttac
    61501 cagacctggc tggcgcggaa cggcgaattt ttctttatag ggcgagcgaa taacgcctat
    61561 ttgctcgaac tggaaactgc tcatttcgcc gtaatgttaa gcgcagagcc gatacataca
    61621 gcctgacgat agcagcccgg cgtaccgctg gtgacttcgc agctatgcag taatacagca
    61681 ttggctttca ttttagaggc attgatttgc atctgcttac gtgcggttgg aatgctcggc
    61741 ggagagtcct gattagaggc ctggcaagag tcgccactga cttcaccgag atcgcggaac
    61801 ggtttgccga ctaattcttc tgcattggta taaattcgga ccggcgtggc gcgcggcgct
    61861 ttcggttttg caggctccgc tttcggctgg ggtgcagtgc tttgaacggg ttcgacaggg
    61921 gatctgctta acatggaaca gccgcttagc atgagtgcta ctaaacagat cggtaaagca
    61981 cgcatagtat ttcctcaatg tatgatcaaa acgtcaatat tgaatcagga gcttgtaaaa
    62041 atgacaagac gggcaagcgc ccgtcctgaa tgatattaca aattgtggaa acagcctaaa
    62101 aattaccagc ctttaacagc gccgccgtta aacactttgt ttgctgcttc gtaaacttca
    62161 tcagactgat aagcctggac gaatttcttc acgttctcgg cgtctttgtt atcttcacgc
    62221 gttacgatca ggtttacgta tggggagtct ttatcttcga caaagatacc gtctttcgcc
    62281 ggagtcaggc caatctggct ggcataggtg gtattgataa ctgccagagc gatttgcgcg
    62341 tcgtccagag aacgcggcag ttgcggtgct tccagttcaa caattttcag attttttggg
    62401 ttctcaacaa catcaagaac ggtcggcagc aggccaacgc catctttcag tttgatcaag
    62461 cccacttttt gcagcagcag cagtgaacga ccaagattag ttggatcgtt tggcacggca
    62521 acctgcgaac catcctgcag ttcatccagt gatttgattt tcttggagta accagcaatc
    62581 ggataaacaa aagtgttgcc tactgcgacc agtttgtagc cacgatcttt cagttgctga
    62641 tcaaggtacg gtttatgctg gaaggcgttg gcgtcgatat cgcctttgct caatgcttcg
    62701 tttggcagaa catagtcgtt gaaggttacc agctcaacgt ccaggccata tttgtctttc
    62761 gcaactttct gcgcgacttc tgctacctgc tgttcggcac caacaatcac gccgacttta
    62821 atgtggtttg gatctttttc atcctgaccg cagcctacca gtgccagtga tccaatcagg
    62881 gctcccactg ccgcaaaggt tttgaatttg aacgccatac cttattcctt cttcaattat
    62941 ttatgttgtg ttgaacgtta cttgcgagtg acagcccgga cgatgcggtc gcctgcaaac
    63001 tgaattaaat aaaccagaat gaccagcaat accagtaccg tattcatcac cgtcgcgttg
    63061 tagccgatgt agccatactg atagccaatc tgacctaaac caccggcacc gactgcacca
    63121 cccatcgcgg aataaccgac cagggtaatc agggtgatag ttgccgcatt caccagaccc
    63181 ggcaacgctt ccggtaacag cactttacag acgatctgca ttggcgtggc acccattgcg
    63241 cgggaagctt caattaaccc ggttgggatc tccagcagag cgttctcgac catacgggca
    63301 ataaacggtg ctgcaccaac ggttaacgga acaattgctg cctgcaaacc aatcgatgta
    63361 ccgacaataa cgcgggtaaa cggaatcatc cataccagca agataatgaa cgggatggaa
    63421 cggaaaatgt tcacaatcgc agaaacggta cgatacagct tcgcgttagc aataatttgc
    63481 cccggacgcg tgacataaag cagaacgcca accggcagac caatcacaaa cccaaaaaat
    63541 ccggatacga aggtcattgc cagcgtttcc catacgccac gaaccagcag ccacatcatc
    63601 ggctcagaca taacccagta cctctacttt tacatggtgt tcctgcagcc aggcaatggc
    63661 ggcttgcgta tcttgttgtg tgccgtgcat ttcagtcagc atgatgccga atttaacgcc
    63721 accggcgtag tccatctgcg cgctaataat gttgttgttg acgttgaaac gacgcgcggt
    63781 ttcagaaagc agtggggcat cgaccgattg accggtaaac tccagacgca gcatcggcac
    63841 gcagtcagta aatggctccg tttgcagacg ttcctggtaa tcttccggga tatccagatg
    63901 cagggtcgac tgaataaact tctgcgccag cggcgttttc ggatgcgaga acacttcact
    63961 taccgtgtcc tgctcgatca gttcgccatt gctgatgacc gccacgcaat cacaaatacg
    64021 cttcacaacg tccatttcgt gggtgatcaa cagaatcgtc aaacccagac ggcggttaat
    64081 gtctttcagc agttcgagaa tagaacgtgt cgttgccggg tccagcgcgc tggtggcttc
    64141 atcacacagc aatactttgg gattgctggc taatgcacgg gcaatcgcca cacgttgttt
    64201 ctgcccaccg gaaagattcg acgggtagct atcatgctta tcgccaagac caaccaatga
    64261 cagcaattcc gtcacgcgac gtttgatctc gtctttcggt gtgttgtcca gctccagcgg
    64321 cagagccacg ttgccaaaaa cagtacgcga agagagcagg ttaaaatgct ggaaaatcat
    64381 accaatctgg cggcgagctt tggtcaactc ggattctgac agcgtggtca attcctggcc
    64441 atcgaccagc acgctaccct cggttgggcg ctccagcaag tttacacaac gtataagcgt
    64501 actcttaccc gcgcctgagg caccgataac gccataaatt tgtccagctg gcacatgcag
    64561 gctgacgttg ttcaacgcct ggatggtgcg ggtgccctgg tggaacactt tggtgatatt
    64621 cgaaagtttt atcattgatt atttattatc gtcattaagt tagtcgtagc atctcgaatg
    64681 cctgaaacgg gcaacgccgt caatgaaatg gatgttaagg catccagacg tctaaatcaa
    64741 tcaggtttat gcgaagagca ctttcttgca ggtcgaaaca tgcgatacta gcgtcccatg
    64801 ccttattaag gagctataaa aggtggcgaa gagcgtaccc gcaatttttc ttgaccgtga
    64861 tggcaccatt aatgtcgatc acggctatgt ccatgagatc gacaactttg aatttatcga
    64921 cggtgttatt gacgccatgc gcgagctaaa aaaaatgggc tttgcgctgg tggtagtaac
    64981 caaccagtct ggcattgctc gcggtaaatt taccgaagca cagtttgaaa cgctgaccga
    65041 gtggatggac tggtcgctgg cggaccgaga tgtcgatctg gatggtatct attattgccc
    65101 gcatcatccg caggggagtg ttgaagagtt tcgccaggtc tgcgattgcc gcaaaccaca
    65161 tccggggatg cttttgtcag cacgcgatta tttgcatatt gatatggccg cttcttatat
    65221 ggtgggcgat aaattagaag atatgcaggc agcggttgcg gcgaacgtgg gaacaaaagt
    65281 gctggtgcgt acgggtaaac ctattacacc tgaagcagaa aacgcggcag attgggtgtt
    65341 aaatagcctg gcagacctgc cgcaagcgat aaaaaagcag caaaaaccgg cgcaatgatt
    65401 aaaagatgaa cggttgaaat aaaaatgcat ttttccgctt gtcttcctga gccgactccc
    65461 tataatgcgc ctccatcgac acggcggatg tgaatcactt cacacaaaca gccggttcgg
    65521 ttgaagagaa aaaatcctga aattcagggt tgactctgaa ataggaaagt gtaatatacg
    65581 ccacctcgcg acagtgagct gaaagctgcg tcgcaactgc tctttaacaa tttatcagac
    65641 aatctgtgtg ggcactcgaa gatacggatt cttaacgtcg caagacgata aatgaatacc
    65701 aagtctcaag agtgaacacg taattcatta cgaagtttaa ttctttgagc atcaaacttt
    65761 taaattgaag agtttgatca tggctcagat tgaacgctgg cggcaggcct aacacatgca
    65821 agtcgaacgg taacaggaag cgcttgctgc ttcgctgacg agtggcggac gggtgagtaa
    65881 tgtctgggaa actgcctgat ggagggggat aactactgga aacggtagct aataccgcat
    65941 aacgtcgcaa gaccaaagag ggggaccttc gggcctcttg ccatcggatg tgcccagatg
    66001 ggattagctt gttggtgggg taacggctca cc
//
LOCUS       DAEBXN010000006        62270 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.006, whole genome shotgun sequence.
ACCESSION   DAEBXN010000006 DAEBXN010000000
VERSION     DAEBXN010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 62270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62270
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.006"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            99..1585
                     /locus_tag="KJL99_000371"
                     /pseudo
     CDS             99..1585
                     /locus_tag="KJL99_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            1600..2334
                     /gene="ypdB"
                     /locus_tag="KJL99_000372"
     CDS             1600..2334
                     /gene="ypdB"
                     /locus_tag="KJL99_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HBD4435249.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            2347..3204
                     /locus_tag="KJL99_000373"
                     /pseudo
     CDS             2347..3204
                     /locus_tag="KJL99_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(3207..5702)
                     /gene="ptsP"
                     /locus_tag="KJL99_000374"
     CDS             complement(3207..5702)
                     /gene="ptsP"
                     /locus_tag="KJL99_000374"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBD4435250.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(5727..6764)
                     /gene="ypdE"
                     /locus_tag="KJL99_000375"
     CDS             complement(5727..6764)
                     /gene="ypdE"
                     /locus_tag="KJL99_000375"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBD4435251.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEAARLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARTYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLSCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTLVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(6764..7847)
                     /gene="ypdF"
                     /locus_tag="KJL99_000376"
                     /pseudo
     CDS             complement(6764..7847)
                     /gene="ypdF"
                     /locus_tag="KJL99_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(7862..9109)
                     /locus_tag="KJL99_000377"
     CDS             complement(7862..9109)
                     /locus_tag="KJL99_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBD4435252.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAII
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(9131..9457)
                     /locus_tag="KJL99_000378"
     CDS             complement(9131..9457)
                     /locus_tag="KJL99_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HBD4435253.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQVLEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(9676..10641)
                     /gene="glk"
                     /locus_tag="KJL99_000379"
     CDS             complement(9676..10641)
                     /gene="glk"
                     /locus_tag="KJL99_000379"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HBD4435254.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDYPGLLGSGAHLRQTLGHIL"
     gene            10845..12101
                     /locus_tag="KJL99_000380"
     CDS             10845..12101
                     /locus_tag="KJL99_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HBD4435255.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPR
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            12216..12542
                     /locus_tag="KJL99_000381"
     CDS             12216..12542
                     /locus_tag="KJL99_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HBD4435256.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(12683..13921)
                     /locus_tag="KJL99_000382"
     CDS             complement(12683..13921)
                     /locus_tag="KJL99_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HBD4435257.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLVMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            14257..15459
                     /gene="nupC"
                     /locus_tag="KJL99_000383"
     CDS             14257..15459
                     /gene="nupC"
                     /locus_tag="KJL99_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HBD4435258.1"
                     /translation="MDRVLHFVLALAVVVILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(15509..17621)
                     /locus_tag="KJL99_000384"
                     /pseudo
     CDS             complement(15509..17621)
                     /locus_tag="KJL99_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
     gene            complement(17829..17904)
                     /locus_tag="KJL99_000385"
     tRNA            complement(17829..17904)
                     /locus_tag="KJL99_000385"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17869..17871),aa:Ala,seq:ggc)
     gene            complement(17944..18019)
                     /locus_tag="KJL99_000386"
     tRNA            complement(17944..18019)
                     /locus_tag="KJL99_000386"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17984..17986),aa:Ala,seq:ggc)
     gene            18240..18599
                     /locus_tag="KJL99_000387"
     CDS             18240..18599
                     /locus_tag="KJL99_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HBD4435259.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            18601..18993
                     /locus_tag="KJL99_000388"
     CDS             18601..18993
                     /locus_tag="KJL99_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HBD4435260.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWTREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(19045..20460)
                     /gene="gltX"
                     /locus_tag="KJL99_000389"
     CDS             complement(19045..20460)
                     /gene="gltX"
                     /locus_tag="KJL99_000389"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HBD4435261.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            20719..20794
                     /locus_tag="KJL99_000390"
     tRNA            20719..20794
                     /locus_tag="KJL99_000390"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20752..20754,aa:Val,seq:tac)
     gene            20839..20914
                     /locus_tag="KJL99_000391"
     tRNA            20839..20914
                     /locus_tag="KJL99_000391"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20872..20874,aa:Val,seq:tac)
     gene            20919..20994
                     /locus_tag="KJL99_000392"
     tRNA            20919..20994
                     /locus_tag="KJL99_000392"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20952..20954,aa:Lys,seq:ttt)
     gene            21025..21150
                     /locus_tag="KJL99_000393"
     ncRNA           21025..21150
                     /ncRNA_class="other"
                     /locus_tag="KJL99_000393"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            21270..21611
                     /locus_tag="KJL99_000394"
     CDS             21270..21611
                     /locus_tag="KJL99_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HBD4435262.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(21602..22528)
                     /locus_tag="KJL99_000395"
     CDS             complement(21602..22528)
                     /locus_tag="KJL99_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBD4435263.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            22618..23616
                     /locus_tag="KJL99_000396"
     CDS             22618..23616
                     /locus_tag="KJL99_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HBD4435264.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGARGS
                     LEQVGKIMLQLLLPFVLVHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLSFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(23613..23831)
                     /locus_tag="KJL99_000397"
     CDS             complement(23613..23831)
                     /locus_tag="KJL99_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HBD4435265.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(23833..25848)
                     /gene="ligA"
                     /locus_tag="KJL99_000398"
     CDS             complement(23833..25848)
                     /gene="ligA"
                     /locus_tag="KJL99_000398"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HBD4435266.1"
                     /translation="MESIEQQLTELRTTLCHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGVPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKLAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(25919..26905)
                     /gene="zipA"
                     /locus_tag="KJL99_000399"
     CDS             complement(25919..26905)
                     /gene="zipA"
                     /locus_tag="KJL99_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HBD4435267.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYLPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            27135..27896
                     /gene="cysZ"
                     /locus_tag="KJL99_000400"
     CDS             27135..27896
                     /gene="cysZ"
                     /locus_tag="KJL99_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HBD4435268.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTFMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFVWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            28081..29052
                     /gene="cysK"
                     /locus_tag="KJL99_000401"
     CDS             28081..29052
                     /gene="cysK"
                     /locus_tag="KJL99_000401"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HBD4435269.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQVLAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            29436..29693
                     /gene="ptsH"
                     /locus_tag="KJL99_000402"
     CDS             29436..29693
                     /gene="ptsH"
                     /locus_tag="KJL99_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HBD4435270.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            29738..31465
                     /gene="ptsI"
                     /locus_tag="KJL99_000403"
     CDS             29738..31465
                     /gene="ptsI"
                     /locus_tag="KJL99_000403"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HBD4435271.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            31506..32015
                     /gene="crr"
                     /locus_tag="KJL99_000404"
     CDS             31506..32015
                     /gene="crr"
                     /locus_tag="KJL99_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBD4435272.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(32057..32908)
                     /gene="pdxK"
                     /locus_tag="KJL99_000405"
                     /gene_synonym="thiJ"
     CDS             complement(32057..32908)
                     /gene="pdxK"
                     /locus_tag="KJL99_000405"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HBD4435273.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            33013..33387
                     /locus_tag="KJL99_000406"
     CDS             33013..33387
                     /locus_tag="KJL99_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HBD4435274.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAEGN"
     gene            33420..34143
                     /locus_tag="KJL99_000407"
                     /pseudo
     CDS             33420..34143
                     /locus_tag="KJL99_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(34347..35258)
                     /gene="cysM"
                     /locus_tag="KJL99_000408"
     CDS             complement(34347..35258)
                     /gene="cysM"
                     /locus_tag="KJL99_000408"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HBD4435275.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSCFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNAALVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(35392..36489)
                     /gene="cysA"
                     /locus_tag="KJL99_000409"
     CDS             complement(35392..36489)
                     /gene="cysA"
                     /locus_tag="KJL99_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HBD4435276.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNITFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQHGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(36479..37354)
                     /gene="cysW"
                     /locus_tag="KJL99_000410"
     CDS             complement(36479..37354)
                     /gene="cysW"
                     /locus_tag="KJL99_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HBD4435277.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(37354..38187)
                     /gene="cysT"
                     /locus_tag="KJL99_000411"
     CDS             complement(37354..38187)
                     /gene="cysT"
                     /locus_tag="KJL99_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HBD4435278.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(38187..39203)
                     /gene="cysP"
                     /locus_tag="KJL99_000412"
     CDS             complement(38187..39203)
                     /gene="cysP"
                     /locus_tag="KJL99_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HBD4435279.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(39507..40298)
                     /gene="ucpA"
                     /locus_tag="KJL99_000413"
     CDS             complement(39507..40298)
                     /gene="ucpA"
                     /locus_tag="KJL99_000413"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HBD4435280.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     CRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(40485..41285)
                     /gene="murR"
                     /locus_tag="KJL99_000414"
     CDS             complement(40485..41285)
                     /gene="murR"
                     /locus_tag="KJL99_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HBD4435281.1"
                     /translation="MLYLTKIRNAESEFTGNEQKIADFLRARVSELKSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQHQ"
     gene            41449..42345
                     /gene="murQ"
                     /locus_tag="KJL99_000415"
     CDS             41449..42345
                     /gene="murQ"
                     /locus_tag="KJL99_000415"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBD4435282.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPPAVDRV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            42349..43773
                     /gene="murP"
                     /locus_tag="KJL99_000416"
     CDS             42349..43773
                     /gene="murP"
                     /locus_tag="KJL99_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HBD4435283.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGDAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(43953..44852)
                     /gene="yfeX"
                     /locus_tag="KJL99_000417"
     CDS             complement(43953..44852)
                     /gene="yfeX"
                     /locus_tag="KJL99_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708282.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HBD4435284.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDSGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(44948..45523)
                     /locus_tag="KJL99_000418"
     CDS             complement(44948..45523)
                     /locus_tag="KJL99_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HBD4435285.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTTNGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(45584..46033)
                     /locus_tag="KJL99_000419"
     CDS             complement(45584..46033)
                     /locus_tag="KJL99_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HBD4435286.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(46020..46445)
                     /locus_tag="KJL99_000420"
     CDS             complement(46020..46445)
                     /locus_tag="KJL99_000420"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HBD4435287.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            46659..47528
                     /gene="amiA"
                     /locus_tag="KJL99_000421"
     CDS             46659..47528
                     /gene="amiA"
                     /locus_tag="KJL99_000421"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HBD4435288.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            47532..48431
                     /gene="hemF"
                     /locus_tag="KJL99_000422"
     CDS             47532..48431
                     /gene="hemF"
                     /locus_tag="KJL99_000422"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HBD4435289.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCQPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(48437..49489)
                     /gene="eutR"
                     /locus_tag="KJL99_000423"
                     /pseudo
     CDS             complement(48437..49489)
                     /gene="eutR"
                     /locus_tag="KJL99_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
     gene            complement(49535..50035)
                     /gene="eutK"
                     /locus_tag="KJL99_000424"
     CDS             complement(49535..50035)
                     /gene="eutK"
                     /locus_tag="KJL99_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HBD4435290.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDIQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVCQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(50048..50707)
                     /gene="eutL"
                     /locus_tag="KJL99_000425"
     CDS             complement(50048..50707)
                     /gene="eutL"
                     /locus_tag="KJL99_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HBD4435291.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            51889..52680
                     /locus_tag="KJL99_000426"
     CDS             51889..52680
                     /locus_tag="KJL99_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4435292.1"
                     /translation="MRALKIDAISLNFSHSPEWWRSGLIATLPRLDIERIIDTYIKRK
                     RDKFIVKIRGLHIMRISEQQRQILDYLAKNESITARQAADLVYGGNVTRVQVDAARRS
                     LMTMVSNGLIRKQGRAFVAERHINHAYSQDVEELRRQILWVFSKQGQEYFLSEHPNFA
                     AKELTVSGLSCALDYLVGEGCVTYKQLWHALNTLVEDGLLIATRENIEYWIMPRSGRR
                     NHARYISGNRYRTPDFNPTQEDRDRAEMMCREQEKRIMDKLFSMQ"
     gene            53400..53795
                     /locus_tag="KJL99_000427"
     CDS             53400..53795
                     /locus_tag="KJL99_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBD4435293.1"
                     /translation="MTNIERLERCADIMRRRWIYDPEAGLLFSRETKDVIKGSMTNKG
                     HLVVTVHEGKFKVTLTYQKACYVYTYGAYDETLYEVHHVNLNKQDNRIKNIRLMPKEK
                     HRRIHKNVRKLIRLGHTQWLQPQLIEQSL"
     gene            53813..54076
                     /locus_tag="KJL99_000428"
     CDS             53813..54076
                     /locus_tag="KJL99_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4435294.1"
                     /translation="MHSLDSYFQRTTAPKSAAQERREEFHEKVMRSADYIADKFVETV
                     RPLVDEVADKLQSEMPEDMEGTAKRRLICELSRRFGVSISAFK"
     gene            54099..54641
                     /locus_tag="KJL99_000429"
     CDS             54099..54641
                     /locus_tag="KJL99_000429"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4435295.1"
                     /translation="MDAFEVLANKGAELVAQRDKTANEGERALLNKQIKAIRMAQFKL
                     ISNEVIETIKPQIPQIVADAIKAAGLEKRIAGLKTNAEKMEMYKQSGAPNPDEYFSPD
                     VEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     ATDPEAEQRKRVFNAIGIRY"
     gene            54780..55067
                     /locus_tag="KJL99_000430"
     CDS             54780..55067
                     /locus_tag="KJL99_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4435296.1"
                     /translation="MLALRIMQGIAKTLAEHVLDLKHSPLSKQAMKRQTLRLWAEYSL
                     GTINKIIDMKSGPSNQSAEEMEFIRRLILIRRDIHSQLHSVGIDINDGTGD"
     gene            55106..55312
                     /locus_tag="KJL99_000431"
     CDS             55106..55312
                     /locus_tag="KJL99_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4435297.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
     gene            55624..56109
                     /locus_tag="KJL99_000432"
     CDS             55624..56109
                     /locus_tag="KJL99_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HBD4435298.1"
                     /translation="MKNKSGNSCYREAVDIHSSLELSFILHKYHLRVDEKKSRNVFSA
                     IRDELILDGDSRQNLATFCQTWLDDEIRELLNLAIDKNMIYKDEYPQMAEPEGRCVRM
                     LSDSWSFPDSRHTLGCSTIGSSEAAMLGRLALKWQWCKKREVQGKSTEPDLRSCANML
                     A"
     gene            56090..56368
                     /locus_tag="KJL99_000433"
     CDS             56090..56368
                     /locus_tag="KJL99_000433"
                     /inference="COORDINATES: protein motif:HMM:NF012503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4435299.1"
                     /translation="MQICWHKFCRYCDVKLCEIPLDDHHLIMNAKEVLKHVDENTIGV
                     VTTLGITYFCKADCVSVFKISVKVNISFYIYIVIICFYFKNVREYKNV"
     gene            56361..57821
                     /locus_tag="KJL99_000434"
     CDS             56361..57821
                     /locus_tag="KJL99_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBD4435300.1"
                     /translation="MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIF
                     YYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYF
                     PTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSK
                     WCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAY
                     AGMEMNAVHVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLL
                     VAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKT
                     NRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIY
                     LRHSQPDTKRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLV
                     ALFLLIPILIYAFRKSDWKDPNSDFEPFSWEKRTSN"
     gene            57855..58664
                     /gene="murI"
                     /locus_tag="KJL99_000435"
     CDS             57855..58664
                     /gene="murI"
                     /locus_tag="KJL99_000435"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001507729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBD4435301.1"
                     /translation="MFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNMNAPY
                     GNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEEYSGYAKSFPFPILSII
                     EPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKKLNSTINVFGEPSKNLAMLIEEGN
                     LNAPAILNDIKKHVNHLISLHPVNEIILGCTHYPIVQNFFEAVAPDIKFINPAHDQAI
                     SIKNHLGQLNLLNSSNIGTLQINTSGSMEIYKTVLGELSITKPHTFSIRQF"
     gene            complement(58783..59477)
                     /locus_tag="KJL99_000436"
     CDS             complement(join(58783..59231,59231..59477))
                     /locus_tag="KJL99_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBD4435302.1"
                     /translation="MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTY
                     QARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPVAHADVA
                     FICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIG
                     MLTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHE
                     KVIGAFIEKHMFY"
     gene            complement(59996..61369)
                     /gene="eutA"
                     /locus_tag="KJL99_000437"
     CDS             complement(59996..61369)
                     /gene="eutA"
                     /locus_tag="KJL99_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HBD4435303.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANYALFDAGKISG
                     TACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQLVQVTRRMAAL
                     IVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPANPFCFADIGPLL
                     ATALHDHPRLREMNVQFPAQTVRSTVIGAGAHTLSLSGSTIWLEGVQLPLRNLPVAIP
                     IDETDLVSAWQQALLQLDLDPKTDVYVLALPASLPVRYAAVLTVINALVDFVARFPNP
                     HPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPLFGGSVVPVTV
                     KSLAFPS"
     gene            complement(61344..61823)
                     /gene="eutP"
                     /locus_tag="KJL99_000438"
     CDS             complement(61344..61823)
                     /gene="eutP"
                     /locus_tag="KJL99_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HBD4435304.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTLAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(61836..62171)
                     /gene="eutS"
                     /locus_tag="KJL99_000439"
     CDS             complement(61836..62171)
                     /gene="eutS"
                     /locus_tag="KJL99_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HBD4435305.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
BASE COUNT        15642 a        16235 c        15221 g        15172 t
ORIGIN      
        1 ccccttttaa atttatggtt tttcctcaaa aacggcctgc tggtcatcag ccttcaggtt
       61 acctatcata gaggtttaat ccttattcag agtcacccgt gcacgaaata ttcaacatgc
      121 tgctggcggt cttcgatcgg gcagcgttaa tgcttatctg cctgttcttt ctcatccgta
      181 tccgcctgtt tcgcgaactg ttgcacaagt cggcgcactc cccaaaagaa ttgctcgccg
      241 ttaccgccat tttctcgctg ttcgccctgt tcagcacctg gtccggcgtt cccgtagaag
      301 gctcgctggt gaacgtacgt attatcgcgg tgatgtccgg cgggattctg tttggcccgt
      361 gggtaggcat cattaccggc gtgattgcgg gtattcaccg gtatttaatt gatatcggcg
      421 gcgtgacggc gatcccctgc tttatcacca gcattctggc gggttgtata tcaggctgga
      481 taaacctgaa aatccccaaa gcacagcgct ggcgctggat atcgccaaca aaacgctacc
      541 gctgtttcgc catgtcaata gcgagtcatt acgcaaggtc tgcgaaatta tccgcgatga
      601 cattcacgcc gatgcggtgg cgattactaa taccgatcat gtgctggcct atgttggcgt
      661 gggtgaacat aactatcaga atggcgatga cttcattagc ccgactaccc gtcaggcgat
      721 gaattacgga aaaatcatca ttaaaaacaa tgatgaagcc caccgcacac cagagattca
      781 ttccatgctg gtgatcccat tgtgggagaa aggggtcgtg accggaacgc tgaaaattta
      841 ctactgccac gcgcatcaga tcacctcgtc attacaggaa atggcggtcg gtctgtcgca
      901 aatcatctcc acgcaactgg aggtttcacg cgccgagcag ctacgtgaaa tggcaaataa
      961 ggcagagatt cgcgccctgc aaagcaaaat taatccccat tttctgttta acgctctgaa
     1021 cgctatttca tcgtcaatcc gtctgaatcc ggataccgct cgccagttga tctttaatct
     1081 gtcgcgttat ctgcgctata acattgaatt aaaagacgat gagcaaatcg atatcaaaaa
     1141 agagctgtat caaattaaag actatattgc cattgagcag gcccgctttg gcgacaagct
     1201 gacggttatc tatgatattg atgaagaggt gaattgctgc attcccagcc tgctgatcca
     1261 gccgttggtg gagaacgcca ttgtccacgg tattcagcct tgcaaaggta aaggcgttgt
     1321 caccatcagc gttgcagagt gcggaaatcg ggtacgcatt gcggtgcgag ataccgggca
     1381 cggcatcgtt ccaaaggtga ttgagcgggt cgaagcgaat gaaatgccgg gcaataaaat
     1441 tggcctgctg aatgtccatc atcgcgtgaa gttattgtat ggcgaggggc tgcatatccg
     1501 ccgcctggag ccggggacgg aaattgcgtt ttacattcct aaccaacgca ccccagtcgc
     1561 ctcacaggct acgttattgc tttgagccgg agtgatattg tgaaagtcat cattgttgaa
     1621 gacgaattcc tggcacaaca ggaactgagc tggctaatta aagagcacag ccagatggag
     1681 attgtcggca cctttgacga cggtctggac gtgttgaagt ttttgcagca taaccgcgtc
     1741 gacgccattt ttctggatat caatattccg tcgctggatg gcgtgttgct ggcgcaaaac
     1801 atcagccagt tcgcccataa accgtttatt gtgttcatca ccgcgtggaa agaacatgcg
     1861 gtagaagcgt ttgaactgga ggcgtttgac tacattctca aaccgtatca ggagtcacgt
     1921 attaccggga tgctgcaaaa actggaagcg gcctggcaac aacagcagac cagcagtacg
     1981 cctgccgcga cggtaacgcg tgagaatgac accattaatc tggtgaaaga tgagcgaata
     2041 atcgtcacgc caattaacga tatctattac gccgaagcgc acgagaaaat gacctttgtc
     2101 tatacgcggc gtgaatccta cgtaatgccg atgaacatta ccgaattttg cagcaaactg
     2161 ccgccgtcgc attttttccg ctgccatcgc tcattttgtg tcaatctgaa caaaatacgc
     2221 gaaatcgaac cgtggtttaa taacacctac attctgcgac tgaaagatct ggattttgaa
     2281 gtgccggtca gccgcagcaa agtgaaagaa tttcgccagt taatgcatct ttaatcggga
     2341 actttcatga aagcaccagg cttgcccgcc gatcaacaat ttttcgccga tctgttcagc
     2401 ggcctagtgc ttaacccgca actactgggg cgcgtctggt ttgccagcca gcctgcctcg
     2461 ttaccggtgg gcagtttatg tattgatttt ccccgtctgg atatcgtgct gcgcggcgaa
     2521 tacggcaacc tgctggaagc aaagcagcag cgaatggtgg aaggagaaat gctgtttatt
     2581 ccggcacgcg cggctaattt accgatcaac aacaaaccgg tgatgctgtt aagcctggtg
     2641 ttcgcaccga cctggcttgg gttatcgttt tacgatagcc gcaccacgtc gttgttgcat
     2701 cctgctcgcc agacccagct tcccagcctg caacgcggtg aaggtgaagc gatgcttacc
     2761 gccctcaccc atcttagccg ttcgccgctg gagcagaata tcattcagcc actggtgtta
     2821 agtttgctgc atctttgccg caacgtggtg aatatgccgc cgggtaattc acaaccgcgc
     2881 ggcgattttc tctatcacag catttgtaac tgggtttagg ataattatgc ccagccgctc
     2941 acccgcgaga gcgtggcgca gttttttaat atcacgccca atcatctgtc aaaactgttt
     3001 gctcagcatg gaacgatgcg ttttatcgag tatgtgcgtt gggtgcgaat ggcgaaggcg
     3061 aggatgattt tgcagaaata tcatctgtca attcatgaag tggcacagcg ttgcggtttt
     3121 ccggatagcg actatttttg tcgcgttttc cggcgtcagt ttagtctgac gccgggagag
     3181 tacagcgccc gttttcaggg ctaacgtcag aaggttaatt ctgtttccag cagcgtcagg
     3241 atactttgtg catcttgcgc ggcaaacaac gactggcgga agtttttatt caccagttta
     3301 cgcgccagct gcgagaagac tttcacatga ttcatccctt cgttagcacc cagcgtcagc
     3361 atgatcacca gttcgacttc gcccatttct gactgccagt caaccggttt cgccagccgg
     3421 gcaatgctga tactggaatg acggatccac tgagatttgg tgtgcgggat cgctacgcca
     3481 aaaccaacgc cggtggtaac aatctcttcc cgctgccaga catcttcttc cagctcaaac
     3541 ggatgttcag tgcgcccgtt aacgccgagg ttgccgcaca ggaactggat cgcctgctct
     3601 ttattgctaa aatcctgatc aacaaagata ttctccagcg ccagcagtgg gcgaacgtct
     3661 tcttccggcg taaaggcggt gagtaacgct tcaatttcct gcgcactgcg gcattcacat
     3721 gcctgacgcg ccagttcccg acacgcctcg ctatccagtt gacgaagctg gcttttcacc
     3781 gccggaatac gcgggctact catactcagc tcatccaggc ccagcccaag cagtagcggc
     3841 agataacggc tttcaccgcc cagttcaccg caaatgccta cccatttgcc ccgctgatgc
     3901 gcagtggtaa ctatttgctg caacatgcgc aggaacgatg gcgtaatcgg gttatatagc
     3961 ggcgatacgc gcgggttatt acgatcgacc gcatacagat actgggtcat atcgttggag
     4021 ccgatactga agaaatcgac ctcatcgcag aagtggtcga tgatgtagca caccgacggc
     4081 acttccacca tgatcccaag cgtaatcgtc tctgcatgac gcaggccatc gcgcttaagc
     4141 tcaacgatcg ctttttggat ctcgcctttc acccataaga tctgatcgag gctgtgaacc
     4201 atcgggatca tcaactgggc gttgccgaaa ctggcggcgc gcaaaatggc ccgcagttga
     4261 gtgcggaaca ggccagcaaa ttccgggtaa atacgtaccg cgcgatagcc gaggaacggg
     4321 ttctcttcct ggggaatatt cagataagga atgcttttat cgccgccgat gtccatcgtg
     4381 cggaagataa tcggcttgtc gcccgccgcc agtagcacct gctggtaggc ttcaaattgc
     4441 tcctgctcgt ccggcgcgct gtcgcgatcc atatagagca tttcggtacg gaacagcccg
     4501 acaccttccg cgccgttggc aaacgcgcct ggcgcttcca gagcggtgcc gatattcgcc
     4561 gcaatatcaa tacgcttgtt atcacgggaa taggccagct gcgcggcagc ctgcgcctgt
     4621 tgtttttggc gtttatccgc cagcgtctgc gcgacctgat aataaccgct caccgcgtca
     4681 ttcgggttaa tcgccagcac gccgcactgg gcgtcaagca ctgcaggttg cccggcataa
     4741 cgggcaatcg catccagcgg caagccactc agtaccggga tcgccgaggc acgggccaga
     4801 atcagtgtat gcgaggtgcg cccggttttc tccagaatca tacccgcaag atttttcaaa
     4861 tcaaggctca aaaactggct tggggttaaa tcttcagcca ccagaatggt cggtttttcc
     4921 agcaccagat tgttgcgcgg cttcagttcc ggccaggtga tatgcagcaa ctgttcgctg
     4981 atatcgcgaa tgtcgctgac ccgctcgcgc agataatcgc tggcagaagc agatagtttg
     5041 gcgcaaacct gctccatatt gctgatgatc gccgccccca gtccctgatg ctgttctgtc
     5101 atcaggcgac ggatattgcc tgcaaattca tcatcctgaa tcagcgacaa atgagcgctg
     5161 aggatagttt tgctttcgcc gtcacgctca cgcaattgct gattcagttg ctcggcaagc
     5221 gttgccaggc tgtgctccag ccgggtggaa tcttgcgcac tggcggggat tgcccgataa
     5281 ctgtcgaggc tgtcgctttg taacagggtc agcgtaccca cgccgacgcc gcttgccagc
     5341 acattgccgt acagtaaatc cgggttaagg cggcttaatg aacgcggcag cggatgcgcc
     5401 gtcagttctg cctgcgtcgg ctgaacgctg tcgctgtcga taaagcgcac caggatgtac
     5461 tcttccagca cgcgccgcgc ctgctcttca tcgctgccgc taatgttcag gctgcaactg
     5521 tcattaaata aggtgccagt tccaatcaac gccagcgagc ttttagcatc agcctttgcg
     5581 ttctggcgat ggttgataaa ggtgatttcg ctttgccact ggctgcactg ttctttaagt
     5641 tcccacgccg gacgagcatg tagaccgtta ggcaaaggac agagaaattg aatcgttaac
     5701 ataaccgctc cgggttaatc aggagatcat ctgaaatccg tcagttgaac aaccgtctca
     5761 cgcgtaagac gttgaataag ggcagataaa agttgctcca tctgcaaaat gtcgcggcaa
     5821 tcggcaatcg atgccgcgca atgtccgtgg cgggtggcag gccccattac caatgtgggt
     5881 acgccagtac cggttaagtg caccgctcca ccgtccgtgc cgccgttact gaacatatcc
     5941 gcctgcaacg gaacgccaat ttctgccgcc acggtttcaa tccacgcggt aagttttggc
     6001 ggcgcaatca gcgatttatc gcttaacacc agcatcggcc cgttaccaat ctgacgatgg
     6061 ttagccgcgc cataatcaaa gtttttcgcc cagcaggcgg tatcaagcac aatggcgacg
     6121 tccggcgaca ccgcgcgggt agcagtttgc ccgccgcgta atcccacctc ttcgctggaa
     6181 cttgccacca gccacacttc cgcaggcagt tcggcgtcgt gcagctcacg tagtaacgtc
     6241 accagcagat agcaactgag gcgatcatca aaggctttgc ccatcacccg ctggtgaggg
     6301 aggacctgaa aagtggtatc gaaggtgacg cgatcgcctg gacgaattcc cgcctgcatc
     6361 acttcgtcat aggtgcgcgc gccaatgtcc acgcgcatgg cgctaacgtc attcccctgc
     6421 cggtcgccgt caagcaggcc tggaattttg cactcttcac gggtggtgat gcgcaccggc
     6481 tgcagctggc gggcagccat gcgcacgttg ccaaccggca gcacgtcaat agcgccttcg
     6541 cgggagatgc tgcgcaccat aaatcccact tcgtccatat gggcgcagat catcaccttc
     6601 ggacctgtcg attcattgag gcggatcagc accgatccca gaccatcaaa tcgcacttct
     6661 ttttgcaggc gagccgcttc ttccagcagg atctgccgca cttcctgttc cgaggaggcg
     6721 atcgcatctg cctcgctcaa cgcttttaat agcgataaat ccattatgcc tctcccgtga
     6781 gcaacactgt tttcggcatg gcgtagagca cttctgcgcc ttgcggggta accagcacaa
     6841 catcttcgat gcgcacgccc ccttgcccta gcaaataaat ccccggctcc acggtcagca
     6901 acatgcctgg ctgtagcgtc gtggtgtccc gcggtgaaaa acgcggatct tcatggactt
     6961 caatgccgat agcgtgacca gtgttatgac cgaaatagtc gccaaatcca gcctcggtaa
     7021 tgacccggcg cgcggcgtcg tcaatctgct ggcagcgcac gccggggcga attgcagaga
     7081 ttgccgcgag ctgtgcctgc aggacaatct gatagacgtc aaacagcggg tgagattcgg
     7141 cgctcacccc ttcgccattc accagcaagg tgcgcgtcat atcagcagta gccctgatac
     7201 agcgcaccaa aatcgagagt gacaaactcg cccgctgcaa caatcttgtc gctggctttg
     7261 ccgtgcggca gcgccccgcg ccagccgctg gcgacaatgg tgtcaaaaga ggctttttct
     7321 gcgccctgct ggcgcataaa ccactccagt tcagcggcta tctcgcgctc gctcatcccc
     7381 gcctgaataa agcggcgaat atgctccgct ccgcgatcgg caatcccaca ggcgaggcgg
     7441 attatctcca cctcctctgg cgttttgatt tgccgcagca catccggcgt ggcgctaacc
     7501 agtttcgcat tgagttcaga cttccagcgg tgcgcggttt cccaactcac ctgctggcct
     7561 tcaaaaccga gcgtttgcaa ctgctcatcg gcaatgattt gattgacgat agtggtaagc
     7621 gtgttcgttg cgtcaagcaa atgcagctgg tagccttgcg cacgggcttc cacctcaacg
     7681 aaatagcgcg aatctaccag aatgtgcgca ctttcacggc taataaccac atagcctgat
     7741 ccggtggaga tccccagatg cggctgtttg ttctgccgtg aggagagaag caccgcatcc
     7801 agttgttgca ccttaagcca gttgcgcagc gaagcgagta atgtcatctg gattttcctt
     7861 cttacaggct atcgattaac aatttgcctt tgcggaacat catcagacgc aggaacacca
     7921 ccatcagcgc agtaatgatt gcccccagcg cgatccccgc catataaacg cccaagttag
     7981 tgaccagcgg ccatgcccag atagcggatt ccgggaacca ttgcactgcg cccagccaga
     8041 cagctgcggt tgagccgaca atcgcgccga ccatatatga cggaatagcg gtgatggggc
     8101 tttccagcgc aaacggaatc gcaccctcac taatccccat aaaggccagg aacatcgcgg
     8161 tcttaccctg cggataaagt tgagcgttga acaggcgttt gccggttaaa cgacggtcaa
     8221 taatggtcgc cagacccaga ccaatcggcg gaataacgat agcgatagaa cgagcggtga
     8281 ccggtaatac gtggtcagtg gtaaagctga aggcaacgaa acctgcggct ttgttgatcg
     8341 ggccaccaag gtcgattgct gtcgccgcgg cgatccccat tgcgtacatc agcgcgccct
     8401 tctcacctgc ggcagtcagc acagtacgga taccgccgtt gatccagcca ccaaagggag
     8461 tgatgacgta gtacatcgcc agcatgacaa aaatggcgga aagaatcggt agcaaaaatg
     8521 tggttttgaa cgctaacagg aaatccggta actggatttt ctggttcatc catttcacaa
     8581 ggtaaccggc aacgatagaa ataatcagcg caccaatgaa ggtggacggc accggcgaag
     8641 aggtcgccca ttgcatagtg ctgggatcga agttcagcag ttgcgtcggc tgggtagaca
     8701 tcaacccgcc gataaaacca gccgggaacg ccagtttgcc accgatagag ttcgccacaa
     8761 atgcagcaaa catcggaatg gcaaaaccaa acaacacgcc gccgaaggac tgcgacagcc
     8821 aggcaaattt aagtaaggag aggtcgaaac cggagaattt cccgctatta agagcatcca
     8881 tgatgccgat ctcagcagga attttaagcc agctataagc aatcagctgg ctgaaagcga
     8941 ggatcacacc gcccataatt aaggtcggca ccatgcgtga aatccctgac atcacatgct
     9001 gcgggagttc accccagaag ctgcttttag aggcctgcgg attcttaact gccgctgccg
     9061 caccggatgc gccaggcaca acggttgcac tgcgtttttt aatggccata acaattatcc
     9121 ctgtcgaata ttactgttgt tcagaggcaa tcatctcttc gatttctttg atgatgcccg
     9181 cagcgttttt aattgcgtcc tgcaaagtga tttcataaac gtcgcgtgat tcgaaacgtt
     9241 cgttatcttc cggggtaact gccacggaat ggatgatgat ggtcgcttcg gcgatatcct
     9301 gcgccgtcag gcgattctgg atgccgtccg cgccctgggt ttcaattttc acttcataac
     9361 cggcttctac cgccgcttct tccagcacct gagcggccat aaaagtgtga gccaggccca
     9421 ttgggcaggc acataaggca atcagtttct tactcatcgt taattcctcg ttcagtgata
     9481 gttgactagt gaaattatga acgtgcaaaa caaatcgccg ttaccagaca aataatgcat
     9541 ttactggaga attaatcccg ctgtacggaa gtgtgacgca ggtcggcctt gtgaggggac
     9601 acaaagaaaa attgaattaa ctgtatgatt taaaagatta tcgggagagt tacctcccga
     9661 tataacagga aggatttaca gaatgtgacc taaggtctgg cgtaaatgtg caccggaacc
     9721 gagaagaccc ggatagtcat ggacgatgag atacaccgga atatcatgga catattcttt
     9781 aaagcgccct ttatcttcaa atgcggcacg gaaaccggag gctttgaaga actcaaggaa
     9841 gcgcggcacg ataccgcccg caataaacac gccgccaaat gtcccgagat tgagcgccag
     9901 attgccgcca aaacggccca taatgacgca aaacagcgac aatgcgcggc ggcaatcggt
     9961 gcagctgtca gccagcgcgc gttcggtaat atcttttggc ttgagatttt ctggcaggcg
    10021 gttgtcagct ttcacaattg cgcgatacaa attcaccagc ccagggccag aaagcacgcg
    10081 ctccgccgaa acatgaccaa tttccgcacg caatatttcg aggataatgg cctcttcttc
    10141 actattcggc gcaaaatcaa cgtgaccgcc ttcgcccggc aagcttaccc aacgcttatc
    10201 gacatggacc agatgcgcaa ccccaagccc cgttccggca ccgtaaaccg caataggctt
    10261 accttcgacc ggttctgcgc caccaaactg aatcagatgc tcttttttca gcatcgggat
    10321 cgccatcgat acagcggtaa aatcgttaat aatttccaga tggctaaaac cgagattctt
    10381 tttcatttcg gcaattgaga acgcccaggt atggttggtc attgccaccc agtcaccggt
    10441 aattgggcaa gcgatggcaa tacagccgtc tttcacctcg accttatgtt cttcaagata
    10501 aacgcgaatg accgcttcga ggctggggta atcaagccct gaataggtct tagcctgcga
    10561 gatttcacca ctggcaatat cacacagagc aagacgtgcg ttggtgccgc ccacatcacc
    10621 gactaatgca tactttgtca ttcttcaact gctccgctaa agtcaaaata attctttctc
    10681 acactgtaaa tacctggggg cataacaaca acgccggaaa ggcaggctcc ctgtaaatat
    10741 cgatctgggt cacacaatta ctttatcgtt tcagcaccaa ttgcagcgat gcctttttgc
    10801 aagctgggca aactaagtat ctgaccccgc ataaggaata gaacatgctc catccgcgag
    10861 ccagaaccat gttgttatta tcgctccccg ccgtggcaat tgggattgcg tccagtctta
    10921 ttctgattgt ggtgatgaaa atcgcctcgg tattacagaa tttgctctgg caacgactgc
    10981 cgggaactct ggggatagcc caggattcac ccctctggat catcggtgta ttaacgctaa
    11041 cgggtattgc ggtggggttg gttatccgtt tcagccaggg tcatgccgga ccagaccccg
    11101 cctgtgaacc gctgatcggc gcaccggttc cgccctctgc gctacctaga cttatcgtag
    11161 cattaattct cggtcttgct ggcggcgtca gcctggggcc ggaacatccg atcatgaccg
    11221 tcaatatcgc ccttgccgtc gccattggcg ctcgtctgtt accgcgcgtc aaccgaatgg
    11281 agtggactat tttagcctct gccggaacca tcggcgcgct gtttggtaca cctgttgcgg
    11341 cggcgttgat attttcgcaa accttaaatg gcagtagtga agttccgcta tgggatcgtc
    11401 tttttgcgcc gttaatggcg gcagcagctg gtgcacttac taccggatta tttttccatc
    11461 cccatttttc actgcccatt gcgcattacg gacagatgga aatgaccgat attctcagcg
    11521 gtgcaattgt cgcggcgatt gccatcgcag cagggatggt cgccgtatgg tgcttaccac
    11581 ggttgcacgc gatgatgaat caaatgaaaa atccggtgct cgtgctgggt attggcggat
    11641 ttattctcgg aattctgggg gttattggtg gaccagtttc gctgtttaaa gggctggatg
    11701 agatgcagca gatggtggca aatcaggctt tcagcaccag cgattacttt ttgctggcgg
    11761 taattaaact tgccgccctg gtcgttgctg ccgccagtgg ctttcgcggt gggcgaatct
    11821 tcccggcagt gtttgtcggc gtggcattag ggttgatgct gcatgagcac gttcccgccg
    11881 ttccagcggc aataaccgtt tcctgcgcta ttctcggcat cgtgctggtg gtaacacgcg
    11941 atggctggtt aagtcttttt atggcggcag tcgttgtacc caataccaca ttgctaccgc
    12001 tgctctgtat cgtcatgctt ccggcatggc tgttattagc aggtaagccg atgatgatgg
    12061 tcaatcgtcc gaagcaacag ccaccccacg ataatgttta gcaaaaaatg ctctccttta
    12121 tgatttaaga gttatggcga tgatacgggg ctttacctcc ccgtaatatt gcgttaacag
    12181 gccgctgacg gcttcgatcg tgaaggagaa taacaatgtt caggtcactg tttctggcgg
    12241 ccgccctgat ggcatttacc ccgcttgcag caaatgcagg tgaaatcacc ctactgccat
    12301 cgatcaaatt acaaattggc gatcgcgatc attacggtaa ttactgggac ggtggtcact
    12361 ggcgagatcg tgattactgg catcgcaatt atgaatggcg caaaaaccgc tggtggcgtc
    12421 atgataatgg ctatcatcgt ggctgggata agcgtaaagc atatgagcgt ggctatcgtg
    12481 aaggctggcg cgatcgtgac gatcatcgcg gaaaaggccg cggacatggg caccgccatt
    12541 aaacgcgtcc tccaatggag cacaatgcct gatgcgacgc tagcgcgtct tatcaggcct
    12601 acaaaccggc tctttaacgc gtagggcgga taaggcgttt acgccgcatc cgccaatagt
    12661 gccagatgcg acgctcattc aactacaatc ccagcgccgt ccccaccaac aaccagatat
    12721 tcagcgccac gaccagcact acaatcaccc agcctgtctg ttttacgcgt ttgctgttca
    12781 ccagatcgcc catcaacttg ctgtcactgg tgaaaatcag cagtggaacc agcgccaggg
    12841 cgataccaaa acttaacagc acctgactca taaccagaat ccgtgtcgga tctaatccca
    12901 tcagaatgac aataaatgac ggcaacatgg tgactgtacg acgcacccac agcgggatat
    12961 ggaagcgaat gaatccctgc atcaccacct gccccgccag tgtccccacc accgttgagg
    13021 acagtccggc agcaaccaga cttaacccaa agaccgttgc cgcagcatgg cttaacagcg
    13081 gttgcagcgt cagataagcc tcatcaagat cggcaacacc agtatgacca gaaaagtgga
    13141 acgccgccgc agctgtagcc atcatcacca gattgacaaa accggcaatc gtcatggcga
    13201 tagccacatc ccatttggtg gcggaataac gttgttgacg cgaaccgcca tgtaaatgct
    13261 gagtgagcga agagtgcaaa taaatcacat gcggcataat cgtcgcccct aacacgcctg
    13321 ctgccaggaa gaccgcctcc gaagtaggta aactcgggat caccattcct ttacccagct
    13381 gcgccaggtt aggctgggag aaaatcaact cgacaatgta agccgcggca acaaacaaca
    13441 gtaacccgcc aatcactttc tccagcggtt tttgcccgcg acgttgcagc attaaaatca
    13501 ggaaagtcgc gatccccgtc agcaccgcgc cctgcaacaa cgaaacacca agaatgagtt
    13561 taaaaccgat cgccgcacca ataaattccg ccaggtcggt tgccatcgca ataatttctg
    13621 cctgaaccca atagaaccac actacgggac gcggatagtg atcacgaatc tgctccgcca
    13681 gatttttacc ggtggcaatc cctagtttgg cagagaggat ctgaatcagc atcgccatca
    13741 ggttggccca aacgacaacc cacagtagct gatagccgaa gctagcaccc gcctgaatat
    13801 tggtcgcaaa gttaccggga tcgatataac caatcgccgc aatgaacgca ggtcccatta
    13861 atgcgagcct catcttacgc gccgcccgtc cgctgctact ctcaacgcga tagttcgtca
    13921 tcttgtgcct ctaaaacata gcctttgcta tgtttcatgc tatgctaaac gagaatgatt
    13981 atcaaattca tttaaatgga ttgtggtgat ttctctgata gaccaagatt atgattacga
    14041 aaagattgat gacttgaatg ttatggttat gtttaatgtt agcacattta cataactttc
    14101 agcttccata cacaacatag cagaaatgta tgacagatca ctatttttga agcctgtcac
    14161 aggacgtcat tatagtgtgt gtcagatctc gttttcctta accatgttac atagaatgtg
    14221 cacggaaatt taacctgcct catatttgga gcaaatatgg accgcgtcct tcattttgta
    14281 ctggcacttg ccgttgttgt gattctcgca ctgctggtaa gcagcgaccg caaaaaaatt
    14341 cgtatccgtt atgttattca actgcttgtt atcgaagtgt tactggcgtg gttcttcctg
    14401 aactccgacg ttggtttagg cttcgtgaaa ggcttctccg aaatgttcga aaaactgctc
    14461 ggatttgcca acgaagggac taacttcgtc tttggtagca tgaatgatca aggcctggca
    14521 ttcttcttcc tgaaagtgct gtgcccaatc gtctttatct ctgcactgat cggtattctc
    14581 cagcacattc gcgtgttgcc ggtgattatc cgcgcaattg gtttcctgct ctccaaagtc
    14641 aacggtatgg gcaaactgga atcctttaac gccgtcagct ccctgattct gggtcagtca
    14701 gaaaacttta ttgcctataa agatatcctc ggcaaaatct cccgtaatcg tatgtacacc
    14761 atggctgcca cggcaatgtc caccgtgtcg atgtccatcg ttggtgcata catgaccatg
    14821 ctggaaccga aatacgtcgt tgctgcgctg gtactgaaca tgttcagcac ctttatcgtg
    14881 ctgtcgctga tcaatcctta ccgtgttgat gccagtgaag aaaacatcca gatgtccaac
    14941 ctgcacgaag gtcagagctt cttcgaaatg ctgggtgaat acattctggc aggtttcaaa
    15001 gttgccatta tcgttgccgc gatgctgatc ggctttatcg ccctgatcgc cgcgctgaac
    15061 gcactgtttg ccaccgttac tggctggttt ggctacagca tctccttcca gggcatcctg
    15121 ggctacatct tctatccgat tgcatgggtg atgggtgttc cttccagtga agcactgcaa
    15181 gtgggcagta tcatggcgac caaactggtt tccaacgagt tcgttgcgat gatggatctg
    15241 cagaaaattg cttccacgct ctctccgcgt gctgaaggca tcatctctgt gttcctagtt
    15301 tccttcgcta acttctcttc aatcgggatt atcgcaggtg cagttaaagg cctgaatgaa
    15361 gagcaaggta acgtggtttc tcgcttcggt ctgaaactgg tttacggctc taccctggtg
    15421 agtgtgctgt ctgcgtcaat cgcagcactg gtgctgtaaa accatacata aaaaagccgg
    15481 ggatcattcc ccggcttttt tacgcctgtt aatcagctaa tggctgcggg cgaccaatca
    15541 aatacccttg cagatattgc accccgagct tatgcaatag cgcctgctgc tgttgcgtct
    15601 cgacaaactc cgcgaccaca ctcaatgact tcgctttcgc cagatcggta attgatctca
    15661 caatcatcgc atccagcgta ttcgtgacaa tatctttcac aaagacgcca tcaattttga
    15721 tgatatcagc ctgcaaacgc tttaaccgtt cgtagttggc atatccggtg ccaaagtcat
    15781 caatcgcaat ccggaaacca aacttatgca gctgctcgat gttgtacatg ctgctttctg
    15841 cgttagaaaa cgcctgctcc tcggtgatct caagaatgac cgcctgcggg gagatgtgat
    15901 aacgcttaaa cagacgaata atccgcccgg caatattctt ttgcagcagc gtgagcggca
    15961 ttaaattgac tgaaaagcgc ggtccttttt tgtcgcaagg gtgtgttgcc agccacttca
    16021 acaaggattc cagcacttgc aaatcaaaac gcgcgctaag gttaaactga gcaataaggg
    16081 gcagaaactt atccggggtc ataatgccgc cgtcatattt cagtcgcgcg aggatctcat
    16141 cataaccttc accctctttg ttgcgaattg gctgggcgta gagcagcaaa tcaccctgat
    16201 ctaacgccgt gcgaatggta ttcagcaata gcacctgttt agtggtctgc ccggaaacca
    16261 tctcctctct gctatccagc gccagcacat gatgatgtgc gcaggattgc tccgccagcc
    16321 agcttaactg ccccaacaag ggttgcaggg tttcctgatt tccatcaaaa cgcccccagg
    16381 cagcaccata gcccatatcc agcccggtat tgttccagtg aatttgccga ctattcagga
    16441 tgttaaccat atgctggagt cgcccttccg tttccggccc gctcagcact aacagcagtt
    16501 cactacccgg caattgatac aacttttcgt tttcctgcat caacggcagc agcgtacggc
    16561 aaattgagcg gatacagtga acgcgcatca ttaagccgta atgacgactc ataaactcaa
    16621 gattatcaat gcgcaggcag caaaaactct tgcccgcctc ttgctccggc gcttgttcca
    16681 acgcacgaaa gttgggtagg agcgttaacg gatctgttag cgcctgcaaa tgccagcggc
    16741 gattaagcca ttcactgcga tgataaatgc gcaccatata gagcaggcaa acgctaaagg
    16801 aaatcagcac cgcgagaata aatgccagcg aatattcggt ttccacccct tgcaaaaagt
    16861 tctgattgta attcagaagg caaagcgttg aaacagccca ggtgagattt aaaaacggat
    16921 agcgaagatt accgaccccg agggtaaaga tgatgaagaa aacgggtacc aggtaaccgg
    16981 caataaagtc gttttcataa ggtgtgcaca acagaagtag cagcacgctt aacgctgcca
    17041 gccagcttaa ggtaaatgcg agtgagatag tagaaaagca tgttgtaaat cagcactgcg
    17101 gtgaaaaggc ttagtaaatc aacgaccgtg aaaatggcat ccgcatcgcc gaaaaaggtg
    17161 gatatcttta gcgggaaatc aaagaagctc cccacaagat acatgctgca tttgatgccg
    17221 atcggcgtca ccaaaccaag ccagaacaga cgttgccaga catatcggct ggtcaatcca
    17281 taacgccaac gcgtccccag ctgccagcgt aatattgcgc aagcacacaa cacgacaaac
    17341 gtctggctgc cgagcatgac cgcagtctgt aaaaaagaga gatgaaagtt ccacagattg
    17401 gtgcaaaaca tcccgcataa aacgggaacg acaccgcgcc agccaaagat aaacaagatt
    17461 gacagcatta cgcacagtgg catccacgcc agaaagatat aactggaatg gataatggca
    17521 agtggcgaaa taaaacggga tagctgaata agtaccacgg tgagcgtaaa cgctagtgtg
    17581 aatatcttga tattttttat caggttatgc tccacaaaca tgagtacact actctcactg
    17641 atcggggttt tcaggcacac aaataataag tttattcgca tcgcgaagca aacaggggcc
    17701 agcctgttgc cgatttttca tccaatgatg ctttcaccag gcggcgctaa cccggacgta
    17761 gactgtatta caaaagcggc aaaaagcaga gacaaaaaac ccccgctttg cagcgagggt
    17821 tggaaatttg gtggagctaa gcgggatcga accgctgacc tcttgcatgc catgcaagcg
    17881 ctctcccagc tgagctatag ccccacgatg cgtttacgta ccaaatttgc tgggtgcaaa
    17941 atttggtgga gctaagcggg atcgaaccgc tgacctcttg catgccatgc aagcgctctc
    18001 ccagctgagc tatagccccg tcacgtaaag cttgtcgagt tgacgggcgg catcatatga
    18061 attccgcccg aatgtgtcaa cggcaaattg caacgtgtag tttcaatcgc tgaaaaatca
    18121 ggcaaatgaa caattttgga aaaccgctcg cattcagtag ttattcatgt cacggtttcc
    18181 tgtaaagtgg tgttataaaa tgaactacta atagacccac atacattcag ggaattgtta
    18241 tgttcaagga gaggatgaca ccagatgaac ttgccagact gaccggctat agccgccaga
    18301 ccattaataa atgggtacgc aaggaaggct ggacaacgtc accaaaacct ggcgtccagg
    18361 gtggcaaagc ccgactggtt cacgtcaatg aacaggttcg tgaatatatt cgcaatgctg
    18421 aacgtccaga aggtcaggga gaagcgcctg ccctttctgg tgatgcgccg cttgaagtat
    18481 tgctggtgac actggcgaaa gagatgacgc cagttgaaca aaaacagttt acatccttgc
    18541 ttctgcggga agggattatc ggattgttac aacgcttagg gattcgcgat agcaaataat
    18601 atgaaaagat tacgcaataa aatgaccacc gaagaactgg ctgaatgcct cggtgtggct
    18661 aaacaaactg ttaaccgttg gaccagagaa aaaggctgga aaacggaaaa atttcccggc
    18721 gtgaaaggcg gtcgtgccag acttattctg gtcgatacgc aagtttgcga gtttattcag
    18781 aacacgccag ccttccataa cacaccaatg ttgatggaag ctgaagagag aattgctgaa
    18841 tacgctcctg gagcacgcgc cccggcgtat cgacaaatca tcaacgcgat agataatatg
    18901 acagacattg agcaggaaaa agtcgcgcaa tttttgtcac gtgaaggaat tcgcaatttc
    18961 ctcgcccgtc tcgatattga cgaatcagca taaataaaaa acggcaggat attatctcct
    19021 gccgtttatc tttttacacg ctaattactg ctgattttcg cgttcagcaa taaaatccag
    19081 cgctttgttg atacgctcga tactgcgggt cttaccaatt gcgtgaacgg taacatccag
    19141 tgctggagac tgccccgcac cggttacggc gacacgcagc ggcataccaa ctttacccat
    19201 acccacttcc agctcatccg ccgtcgcctg aatagcgtga tgaacgtttt cagcggtcca
    19261 gtcagtaatc gcggccagtt tgtcacgaac cacttccagc ggctgacgcg ctaccggacg
    19321 cagatgtttt ttcgccgcat cggcatcgaa ctcagcaaaa tcttcgtaga aataacggca
    19381 gctctgtgcc atctctttca gcgtcttgca gcgttcgccc agcagtttca ccagatcagc
    19441 cagctgcggg ccgttacggg tatcgatatt ttcctgctca atgtgccact gtaagtgagt
    19501 agcaacatac tccggcggca gcgcgttaat gtagtgatgg ttcagccaca gcagcttgtc
    19561 ggtgttgaac gcactggcag atttgctgac ggcattcaaa gtgaagtatt tgatcatctc
    19621 ttcacgagtg aagatttcct gatcgccgtg ggaccagccc agacgcacca gatagttcag
    19681 cagtgcttct ggcaaataac cgtcatcacg atactgcatt acgctgactg ccccgtgacg
    19741 tttggacagt tttttaccgt catcgccatt gatcatagaa acgtgcgcgt aaaccggcac
    19801 cggcgctttc agggccttaa gaatgttgat ctggcgcggc gtgttgttga tatggtcttc
    19861 gccacggata acgtgggtga tttccatatc ccagtcatcg acaaccacac agaagttata
    19921 ggttggggaa ccatcggtac ggcggataat aagatcgtcc agttcctggt tgctgaactc
    19981 gatcggacca cggatctgat cgtcaaaaac aacagaacct tcctgcgggt tagcaaaacg
    20041 tacaacacac ggttcatcat cagcatgatg ctcatggctg tggcggcagc gaccgtcata
    20101 acgcggcttc tcacctttcg ccatttgctc ttcgcgcagc gcttccaggc gctctttaga
    20161 gcaatagcat ttataagcag tgccctcttc cagcatctga tcgatcaccg cgttgtagcg
    20221 atcaaaacgt ttggtctggt agtacggacc ttcatcccac tccaggctca gccagttcat
    20281 gccatccata atggcttcga tagcttccgg cgtggaacgc tcaagatcgg tgtcttcaat
    20341 acgcagcacg aactcaccgc cgtggttacg tgcaaaaagc caggagtaaa gagcagtacg
    20401 cgcgccgcca acgtgcagat agcctgttgg gcttggcgcg aagcgagttt tgattttcat
    20461 gaaatggcct tacgtttaga aagatgccga caaccggcaa atcctggaaa aattgagtgg
    20521 gcgatattct atcactcccg cctgattcct caatgtagtt cgggtttcta ccttacgcat
    20581 tattgttttc gttgacaaat tgcgcaacac ggtctgtttt gcgatcatct tgcttaaatt
    20641 tacgacgaac gaacaatttc tttagaaaat gcgttgactc attttgaact ctccctataa
    20701 tgcgactcca cacagcgggg gtgattagct cagctgggag agcacctccc ttacaaggag
    20761 ggggtcggcg gttcgatccc gtcatcaccc accaactact ttatgtagtc tccgccgtgt
    20821 agcaagaaat tgagaagtgg gtgattagct cagctgggag agcacctccc ttacaaggag
    20881 ggggtcggcg gttcgatccc gtcatcaccc accacttcgg gtcgttagct cagttggtag
    20941 agcagttgac ttttaatcaa ttggtcgcag gttcgaatcc tgcacgaccc accaatgtaa
    21001 aaaagcgccc taaaggcgct tttttgctat ctgcgatact caaagattcg aacctgcagc
    21061 aggtttgagt tgagcgcagc gaaacaacgg agccgctcgc ggcgacggcc cgaagggcga
    21121 gcgaagcgag tcatcctgca cgacccacca atgcaaaaaa gcgccctaaa ggcgcttttt
    21181 tgctatctgc gatttgtaag attgattttt cttctcaagt tccttgttct tctaccgata
    21241 ttacctcgta cagtgacgag gactgaacaa tggccactat ctcttcaact tcaattcctt
    21301 ccatacaaac acagagtagt aacagggcat cacaaggtag cgatgttgct tcacaaatcg
    21361 cgcggataag tcagcaaatc atcaaactca ctcaacaaat taaagagatt gtagacacta
    21421 gcggcagtgc tgaagacaag caaaaacagg ccgaattgat tcaacagcaa atcaccctac
    21481 tggaaacaca actggcgcag ttgcagaaac agcaggcaga aaaggcgcag gagaaagagc
    21541 agcgtttgtc tctgaatgta tcccttctaa atcctgttga aaaaacgact catatcgata
    21601 tctatatctg atacaacgga tccccttccc gaccagccat tagcgcattg ccctggtctc
    21661 gtaccacgtc ccataacgct tcagcggcgg tggaaagtga acggttcttc ctgcgcacca
    21721 gcatcaactg gcgttcaacc accggcgtaa tgcgcttaac caccagtggg ctaccttcag
    21781 gcaatggtag cgccagtgca ggaagaatac tgatgccgat gcctgcggct accatcggga
    21841 acagcgtcgc cggatgtcca atctcctgca caatattcgc ctgaatacca ttgcgcgcca
    21901 gcgctgcgtc aatcagcggt cggctgcctg acgcgtaatc ctgcaacacc aatttcgctc
    21961 cctgtaatgc ctgccagggt acataatctt ccacggccaa agcgctatcg cgatggcaaa
    22021 gcagaaagaa aggctcggaa agaatcgctt cacattgcag atcgcccact gggcctggat
    22081 cgatgacgat gccaaagtcg acatctccct ggcgaatact ttccatcacc cactgctgcg
    22141 gacgatcatg caggacaaac tgaatatctg gatagcggcg atggctttca gcaatgcatt
    22201 gcggaataag atgcgcggaa atggtctggc tggcagcgac ccgaactttt ccgctcagtt
    22261 gttgccccat acgcccggta tcgcgcaacg tgctgttaag ttcatccagc agtcgctcaa
    22321 gacgcaaagc caactgctgc cctgcatctg taagcaccac ttcacgcgtg gttctgtcca
    22381 gcaggcgaac accagtatga ttttccagct ccttcacact gtgacttact gccgactggc
    22441 tcaagccgat acgctctcct gcacgactaa aacttttctc ctgcgctact gtgacgaaaa
    22501 cctttaattg ttttaaagaa taattcatct gttttcttca tgaatggatg caataaatca
    22561 attttatttc tcaaactgga agaagcacaa tagaaccatc gatcatctgg agtctttatg
    22621 aaactttttc gtatcctcga tcctttcacc ttaaccctga tcacggtggt gttgctggcc
    22681 tctttctttc cggccagagg cgatttcgtc cccttctttg aaaatctgac caccgcagct
    22741 attgccctgc tgttctttat gcacggcgcg aagttgtcgc gtgaggcgat tattgctggc
    22801 ggtggtcact ggcgactgca tttgtgggta atgtgcagca ccttcgtgct gtttccgatt
    22861 ctgggtgtac tgtttgcctg gtggaaaccg gtaaatgtcg acccgatgct ctactccggt
    22921 tttctctact tgtgcattct cccggctacc gtgcagtctg caatcgcctt cacgtcaatg
    22981 gcgggcggta acgtcgcggc ggcggtttgt tctgcgtcag catccagcct gctggggatt
    23041 ttcctttcac cattgctggt tggtctggtg atgaatgttc acggtgcaag gggcagcctt
    23101 gagcaggtcg gtaaaattat gctgcaactg ctgctgccgt ttgtgttggt gcatctttcc
    23161 cggccgtgga ttggtgactg ggtgtcgcgc aataaaaaat ggattgcgaa aactgaccag
    23221 acgtccattc tgttggtggt ttatacagcg ttcagcgaag ccgtcgttaa tggtatctgg
    23281 cataaagttg gctggggatc attgctgttt atcgtggtgg tcagctgcgt tcttctggct
    23341 atcgtgattg tagttaacgt ctttatggca cgccgactga gcttcaataa ggcagatgaa
    23401 attactatcg tcttttgtgg ttcgaaaaag agtctggcaa atggcatccc gatggcaaac
    23461 attctgttcc ccacatcggt gatcggtatg atggtgctgc ccctgatgat tttccatcag
    23521 atccaattga tggtctgtgc ggtgctggcg cgtcgataca aacgccagac cgaacagtta
    23581 caggcgcagc aggaaagcag cgccgataaa gcttaaagcg gacgcttcag gggctggacc
    23641 agttgcgtca gcccctcggt tttaatcagc agcgtgattt gcattagctc accgagcttc
    23701 cctgccggga actcatcttt gcgggcaaac cacagcagat actcctccgg caggtcaatt
    23761 aagcgacgcc ctttgtattt gccaaacggc attatcgtat tggctatttc aatcagctgc
    23821 tctttttcca tctcagctac ccagcaaacg cagcatttcc gcttcgtcga tgacttcaat
    23881 gcccagttcc tgcgccttcg ccagtttaga tcctgcagct tcacccgcta tcaccagatc
    23941 ggttttcttc gacacgctgc ccgcgacttt cgcgcccagt tcgaccagtc gagctttagc
    24001 gtcatcacgc gacatctggc ttaagctgcc cgtaagcacc acggttttac cagcaaacgg
    24061 gctgtcaatc tcttccgcgt tgataacgat cggcgcaggc cagtgaacac cttccgccaa
    24121 cagctcgctg atgacattgc ggttgctttc ttcggcaaag aagttgtgaa cgtgggatgc
    24181 aacgacaatg ccaacatcag gcaccttttg cagctcttca atcgaagcgg cttccagcgc
    24241 ttccagcgtg ccgaaatatg ccgccagacc tgctgcggtg gcctcgccga cttcacggat
    24301 gccaagtgca tagaggaagc gagcaaaggt ggtttctttc gctttttcca gcgcgttaac
    24361 cacgttttgt gccaattttg gccccatacg ctccagtccg gtcagtttgc ctgcggtgag
    24421 tttgaacaga tctgccggag tatggacata ttctttttca accagctgat cgatgatttt
    24481 gtcgcccatt ccgtcaacat ccatcgcacg gcgggaaaca aagtgtttca gcgactcttt
    24541 acgctgcgca ccgcaaatca ggccaccggt acagcgggca accgcttcac cttccacacg
    24601 ctcaacgtca gaaccacata ccggacaatg cgtcgggaat acaacctcac gggtatcttc
    24661 cgggcgttca gaaagcacga cgttaaccac ctgcgggatc acgtcgccag cgcggcgaat
    24721 caccacttta tcgccaatgc gtaaaccaag acgttcgatt tcatccgcat tgtgtaaggt
    24781 tgcgttactc accagcacgc cagcaacatg gacaggttcc agacgcgcaa caggcgtaat
    24841 cgcgccagta cgcccaacct gaaactcgac gtcacgcaca aaggtcatct gctcctgcgc
    24901 cgggaattta aacgcaaccg cccagcgcgg ggcacgcgcg acaaagccaa gctgctcctg
    24961 ctgttccagt gagttgacct taatcaccac gccgtcgata tcaaagccca gcgtcgggcg
    25021 gtcttcttcc actttgtgat agaacgccag cacttcttcc gccgattcac aaagcgttac
    25081 ccgatcgctg accggcaccc cccacttttt aaattgcagt aaacggccaa gatgagtatc
    25141 cggcagctcg ccaccttcca gaacaccaac gccatagcag aaaaaagtga gcggtcgctt
    25201 cgctgtaata cgcggatcaa gctgacgcag tgaaccagct gccgcattac gtgggttagc
    25261 aaaaactttc ccgcccgtgc gtcgcgcatc ttcgttaatc ttttcgaacc ccgcctgcgg
    25321 caggaacact tcaccacgca cttccagacg cgccgggata ttctctccgt gcagcttcag
    25381 cggaatggcg cgaatagtac gcacattaga cgtgatatct tccccggtgg tgccatcgcc
    25441 acgggtcgcg gcactgacta aaacgccatt ttcatacaga atactgacgg caaggccatc
    25501 cagcttcagc tcacagcacc aggtgacttt ctcgttgttt ttcagacggt cctgcacacg
    25561 tttgttgaaa gcaagaaagc tttcttcatc aaaaacgtta tccagtgaca gcattggtac
    25621 ttcatggcgt atctggctga aagctgccag cggcgcagca cctacacgtt gggtaggcga
    25681 atcaggcgta atcagttctg gatgtttggt ttccagctcg cgcagttcgc gcatcagcct
    25741 gtcgtattca gcgtcgggaa tttccggcgc atccatcaca tgataaagat attcatgatg
    25801 gcaaagcgtc gttcgcagtt ctgtcagttg ttgttcgatt gattccatat cgcaccatca
    25861 atgctaaaaa cccccgacaa gcgggggttc gaagaggagt taatttgcct taagtgtatc
    25921 aggcgttggc gtctttgact tcgcggatga tgtcctggta ctcgcgcaat ttctgcggag
    25981 tcatcatacg gcgctgatcg tcaagcacga caccgcccac ttcatcggca atatgctgcg
    26041 cagattgcag catcagcttg aagttctgca gctcgtcacc gtaagacggt acctgcataa
    26101 agatagtgac acccggagta gtgaaatcct tcatttcagg atcaaaggtt cccggtttca
    26161 ccatattcgc caggctgaat aacgccgggc cgctgccatc cgggctaaga tgacgatggt
    26221 aaatattcat atcgccaaaa atgaagcccg cttgttgaat gctgttaaga agcagttcac
    26281 cgtttagctc gctaccgtga tgcgccgcga cgttcatgat aatcaccgct tctttgcgct
    26341 tcggtttatc cataactgga gccggttccg ctacaggctc aggctgtggt gccgctacgg
    26401 gttctgcagg ctggaaagcc tgttgtgccg gttgcggtgc tgaatgcaca ggctgcggcg
    26461 ctggcgcgac ctgtggcgaa actggctgct gcaacggctg ttcaggctgc ggctgatagg
    26521 caggctgctg caccggctgc gctggacgcg gagcatgttg cggcggtacc tgcgcttcag
    26581 gcggctgctg gaccggttga cgcggctgcg cagacgcata aggcggtagg tactggtgtt
    26641 gcggcgacgg acgagcagcc tcatgctcct gagcgttagc cggggcatga ttcacgcggt
    26701 gaacacgaac ctcaccaacg ccctcatcat cttcgacatc ctcgtcataa gaatcgtcgt
    26761 cacgttttga cttcattcgt tttaatggcc gatcgcggaa catagaagat cgttctttac
    26821 ggctggtcca gaaaccatgt accagtaaag cgattatggc gatcgcgcca acaatgatta
    26881 atatcagacg caaatcctgc atcattatat tctctgttgt tctaacacct tgccaccacg
    26941 gcaaacattt actcactaag agtatttgcc gattacctca agtgcaagtg cactattaac
    27001 tttcacagca caaagataga tgaaatcgtg ctttttgctg ttttttcgaa catatcctaa
    27061 ctgtccattg cgcaattacc cggtaaaata cgcagaattt tcctgggatt ggtcaaaagg
    27121 agctcatcct gattatggtt tcatcattca catctgcccc acgcagcggt ttttactatt
    27181 ttgcgcaagg ctggaagctc gtctcgcaac ctgggattcg gcgtttcgtt attttaccgc
    27241 tgctggtcaa tattttgttg atggggggcg cattctggtg gctctttaca cagctcgatg
    27301 tctggatccc gactttcatg agttacgttc cggactggct acaatggctg agttatctgt
    27361 tgtggcctct ggctgtcatc tctgtgctgt tagtgtttgg ctatttcttc tccacgattg
    27421 ctaactggat tgccgctccg tttaacggtt tattggctga acaactggaa gcacgattga
    27481 ctggcgctac accgccagat accgggattt tcggtatcat gaaagatgtg ccgcgaatca
    27541 tgaaacgcga atggcaaaaa tttgtctggt atctgccgcg cgcaattgta ttactaattc
    27601 tttacttcat ccctggtatt gggcaaaccg tcgcgccggt actgtggttc ctgtttagcg
    27661 cctggatgtt agccatccag tattgcgatt accccttcga taaccacaaa gtgccgttta
    27721 aagagatgcg caccgccctg cgcacacgca aaatcaccaa tatgcagttt ggtgctttaa
    27781 ccagcctgtt tacgatgatc ccgctgctta atctgttcat catgcccgtt gccgtttgtg
    27841 gcgcgacggc gatgtgggtc gattgctatc gcgataaaca cgcgatgtgg cggtaacaat
    27901 ctaccggtta ttttgtaaac cgtttgtgtg aaacaggggt ggcttatgcc gccccttatt
    27961 ccatcttgca tgtcattatt tcccttctgt atatagatat gctaaatcct tacttccgca
    28021 tattctctga gcgggtatgc tacctgttgt atcccaattt catacagtta aggacaggcc
    28081 atgagtaaga tttttgaaga taactcgctg actatcggtc acacgccgct ggttcgcctg
    28141 aatcgcatcg gtaacggacg cattctggcg aaggtggaat ctcgtaaccc cagcttcagc
    28201 gttaagtgcc gtatcggtgc caacatgatt tgggatgccg aaaagcgcgg cgtgctgaaa
    28261 ccaggcgttg aactggttga accgaccagc ggtaataccg ggattgcact ggcctatgta
    28321 gctgccgctc gcggttacaa actcaccctg accatgccag aaaccatgag tattgaacgc
    28381 cgcaagctgc tgaaagcgtt aggtgcaaac ctggtgctga cggaaggtgc taaaggcatg
    28441 aaaggcgcaa tccaaaaagc agaagaaatt gtcgccagca atccagagaa atacctgctg
    28501 ctgcaacaat tcagcaatcc ggcaaaccct gaaattcacg aaaaaaccac cggtccggag
    28561 atctgggaag ataccgacgg tcaggttgat gtatttattg ctggcgttgg gactggcggt
    28621 acgctgactg gcgtcagccg ctacattaaa ggcaccaaag gcaagaccga tcttatctct
    28681 gtcgccgttg agccaaccga ttctccagtt atcgcccagg tgctggcagg tgaagagatt
    28741 aaacctggcc cgcataaaat tcagggtatt ggcgctggtt ttatcccggc taacctcgat
    28801 ctcaagctgg tcgataaagt cattggcatc accaatgaag aagccatttc taccgcccgt
    28861 cgtctaatgg aagaagaagg tattcttgca ggtatctctt ctggagcagc tgttgccgca
    28921 gcgttgaaac tacaagaaga tgaaagcttt accaacaaga atattgtggt tattctacca
    28981 tcatcgggtg agcgttattt aagcaccgca ttgtttgccg atctcttcac tgagaaagaa
    29041 ttgcaacagt aatgccagct tgttaaaaat gcgtaaaaaa gcaccttttt aggtgctttt
    29101 ttgtggcctg cttcaaactt tcgcccctcc tggcattgat tcagcctgtc ggaactggta
    29161 tttaaccaga ctaattattt tgatgcgcga aattaatcgt tacaggaaaa gccaaagctg
    29221 aatcgatttt atgatttggt tcaattcttc ctttagcggc ataatgttta atgacgtacg
    29281 aaacgtcagc ggtcaacacc cgccagcaat ggactgtatt gcgctcttcg tgcgtcgcgt
    29341 ctgttaaaaa ctggcgctaa caatacaggc taaagtcgaa ccgccaggct agactttagt
    29401 tccacaacac taaacctata agttggggaa atacaatgtt ccagcaagaa gttaccatta
    29461 ccgctccgaa cggtctgcac acccgccctg ctgcccagtt tgtaaaagaa gctaagggct
    29521 tcacttctga aattactgtg acttccaacg gcaaaagcgc cagcgcgaaa agcctgttta
    29581 aactgcagac tctgggcctg actcaaggta ccgttgtgac tatctccgca gaaggcgaag
    29641 acgagcagaa agcggttgaa catctggtta aactgatggc ggaactcgag taatttcccg
    29701 ggttctttta aaaatcagtc acaagtaagg tagggttatg atttcaggca ttttagcatc
    29761 cccgggtatc gctttcggta aagctctgct tctgaaagaa gacgaaattg tcattgaccg
    29821 gaaaaaaatt tctgccgacc aggttgatca ggaagttgaa cgttttctga gcggtcgtgc
    29881 caaggcatca gcccagctgg aaacgatcaa aacgaaagct ggtgaaacgt tcggtgaaga
    29941 aaaagaagcc atctttgaag ggcatattat gctgctcgaa gatgaggagc tggagcagga
    30001 aatcatagcc ctgattaaag ataaacacat gacagctgac gcagctgctc atgaagttat
    30061 cgaaggtcag gcttctgccc tggaagagct ggatgatgaa tacctgaaag aacgtgcggc
    30121 tgacgtacgt gatatcggta agcgcctgct gcgcaacatc ctgggcctga agattatcga
    30181 cctgagcgcc attcaggatg aagtcattct ggttgccgct gacctgacgc cgtccgaaac
    30241 cgcacagctg aacctgaaga aggtgctggg tttcatcacc gacgcgggtg gccgtacttc
    30301 ccacacctct atcatggcgc gttctctgga actgcctgct atcgtgggta ccggtagcgt
    30361 cacctctcag gtgaaaaatg acgactatct gattctggat gccgtaaata atcaggttta
    30421 cgtcaatcca accaacgaag ttattgataa aatgcgcgct gttcaggagc aagtggcttc
    30481 tgaaaaagca gagcttgcta aactgaaaga tctgccagct attacgctgg acggtcacca
    30541 ggtagaagta tgcgctaaca ttggtacggt tcgtgacgtt gaaggtgcag agcgtaacgg
    30601 cgctgaaggc gttggtctgt atcgtactga gttcctgttc atggaccgcg acgcgctgcc
    30661 cactgaagaa gaacagtttg ctgcttacaa agcagtggct gaagcgtgtg gctcgcaggc
    30721 ggttatcgtt cgtaccatgg acatcggcgg cgacaaagag ctgccataca tgaacttccc
    30781 gaaagaagag aacccgttcc tcggctggcg cgctatccgt atcgcgatgg atcgtaaaga
    30841 gatcctgcgc gatcagctcc gcgctatcct gcgtgcctcg gctttcggta aattgcgcat
    30901 tatgttcccg atgatcatct ctgttgaaga agtgcgtgca ctgcgcaaag agatcgaaat
    30961 ctacaaacag gaactgcgcg acgaaggtaa agcgtttgac gagtcaattg aaatcggcgt
    31021 aatggtggaa acaccggctg ccgcaacaat tgcacgtcat ttagccaaag aagttgattt
    31081 ctttagtatc ggcaccaatg atttaacgca gtacactctg gcagttgacc gtggtaatga
    31141 tatgatttca cacctttacc agccaatgtc accgtccgtg ctgaacttga tcaagcaagt
    31201 tattgatgct tctcatgctg aaggcaaatg gactggcatg tgtggtgagc ttgctggcga
    31261 tgaacgtgct acacttctgt tgctggggat gggtctggac gaattctcta tgagcgccat
    31321 ttctatcccg cgcattaaga agattatccg taacacgaac ttcgaagatg cgaaggtgtt
    31381 agcagagcag gctcttgctc aaccgacaac ggacgagtta atgacgctgg ttaacaagtt
    31441 cattgaagaa aaaacaatct gctaatccac gagatgcggc ccaatttact gcttaggaga
    31501 agatcatggg tttgttcgat aaactgaaat ctctggtttc cgacgacaaa aaggataccg
    31561 gaactattga gatcattgct ccgctctctg gcgagatcgt caatatcgaa gacgtgccgg
    31621 atgtcgtttt tgcggaaaaa atcgttggtg atggtattgc tatcaaacca acgggtaaca
    31681 aaatggtcgc gcctgtagac ggtaccattg gtaaaatctt tgaaaccaat cacgcgttct
    31741 ctatcgaatc tgatagcggc gttgaactgt tcgtccactt cggtatcgac accgttgaac
    31801 tgaaaggcga aggcttcaag cgtattgctg aagaaggtca gcgcgtgaaa gttggcgata
    31861 ctgtcattga atttgatctg ccgctgctgg aagagaaagc caagtctacc ctgactccgg
    31921 ttgttatctc caacatggac gaaatcaaag aactgatcaa actgtccggt agcgtaaccg
    31981 tgggtgaaac cccggttatc cgcatcaaga agtaattctt gccgcagtga aaaatggcgc
    32041 ccatcggcgc cattttttat gcttccgcca gcggcggcaa aatcaattca tcgctctcat
    32101 gctgctgggt gtagcgcatc acttccagta cgcgcaaccc cgcacggtgc accgcatcgt
    32161 ttaacgcctt ccctttcagc aaaccactga taagctgagc acaaaacagg tcgccagtcc
    32221 ctttcaggtc ggtttttacc cgtgaatggg aaatgacatt cacgctgtcg gcactgacca
    32281 ccaccacctg catctcctga ttttcttcat taccggaggc gctggtaatc accacccatt
    32341 ttaatgtgtc tgaaagcaga ctttttgcgg cagcaatggc actgtcgaga tcgcggcaat
    32401 ttttaccggt caggatttcc agctcaaaga tattgggggt aattccctgc gccagcggca
    32461 gtaaatattg tcgatacgcc tcgggaaggt caggtttgac ataaattccg ctatcaatat
    32521 cgccaatcac cggatcgacc atgatcaata ggtcaggatg gtctttgcgt agcgcagtca
    32581 gccactcggc aaggattttg atttgcgatg ccgttcccat atagcccgtg gttacagcac
    32641 gaagttggcg cagcgcatca cgctcctgaa gcgcacgcaa atagccgcta aaccattcgt
    32701 ccggaatcgc accaccgtag aaagtgtcat aatgcggcgt attgctcagc aataccgtcg
    32761 gcacggcaaa gacattcaag ccgttctgtt ttatagcagg caccgcaatg ctgttgccta
    32821 cgcttccgta cactacctgc gactgcacgg cgacgatatc cgcctgcagc gccctactct
    32881 tatcgttaaa caacaacaaa ctactcattt aattttttct ccttgccgat gatcctcatc
    32941 gtattccaac cgaaacttta cctgattctg gcagtcaaat cggctattac aaaacaaaga
    33001 taaggtaatt cagtgaagaa aatcatttgt ctggccatta cgctgctaat gacactcccc
    33061 gcttacgcga agttaactgt tcatgaagaa gcccgcatca acgccatgct ggagggatta
    33121 gcacagaaaa aggatttgat atttgtgcgc aacggtgatg aacatacctg cgatgaagcg
    33181 gtttcccacc tgcgtctgaa gctcggcaat acccgtaacc gcattgacac tgccgagcag
    33241 tttattgata aggtagcttc gtcgtcatcg attaccggga agccgtatat cgtgaagatc
    33301 cccggtaaga gcgatgagaa cgcacagcct tttttacatg cgttaattgc gcagacggat
    33361 aaaacggtgc ctgcggaagg aaattaatcc gctttggtaa ggcatttaca ggaggtaaca
    33421 tgaaaaaatg ctttatttat cacgatgaaa aatcgaataa attttggtgg atagattacg
    33481 aaggggatag tttagctgtc aactatggca aggtaggtag tattggtaaa ttccatacaa
    33541 aagaattcga taatgaagaa cagtgtctga aagaagccag taaattgatt gccgcaaaaa
    33601 tgaaaaaagg atatcaagaa actccagact ttaacttcat ggatctctac tattttgatg
    33661 atgaagagat tgggttacat cttaaaacgt cacacccaaa cttccagtgc cattttactg
    33721 atccacttta tatgtgttgc tgggatgaag aatctccttt tggtagcgat gaaggtgctg
    33781 atgctctaaa cgaacttgaa aatagcctcc gttctggact gtgctgattt ccctcaaatg
    33841 ttaattgaaa ctatgtgggg tatgaaatac atcgctatgg acagtattct tgaagaggat
    33901 gtgcgtgcgc aattactagc cgatgaaatg agctctatcc agagcaatat gattacctac
    33961 gcgactgcat tcggtcagat caaagtcatg ggtaaaatct cccataaact taaaaagatg
    34021 ggactcaatg cactagcgcg tcatcagctt accgcaaaaa ttcttcaatg gggtgacggt
    34081 caggactcac caatacttca aaaaatgatt gatgacctta ctgcttttcc acacgaaaat
    34141 taaaaactgc atttgtcggc agcaacaact gccgacaaat cagattattg acaaagtgca
    34201 ctttgtttat gccggatgcg gcgtaaacgc ctgatccggc ataaaaatat tgctaattca
    34261 atatattgca gaaacattgt aggcctgata agcggagcgc atcaggcagt tttgcgtttg
    34321 tcatcagtct ccaatgctat taatccttaa atccctgccc cctgactaaa atgctcttcc
    34381 ccaaacactc cggtggagag gtagcgatcg ccacgatcgc agatgatcgc taccaccacc
    34441 gcaccagggt tagcttttgc cacccgcagt gctccagcaa ccgcgccgcc tgagctgacg
    34501 ccacagaata ttccttcccg caccgccagt tcgcgcatgg tgttttccgc atcgcgctga
    34561 tgaatatcca gcacctcatc caccagcgca gcgttgaaaa tccccggcag atattccgca
    34621 ggccagcggc gaatgcccgg aatactgctg ccttcttccg gttgcaggcc aacaatggtc
    34681 accggtttgg attgttcgcg cataaagcat gaaacgccgg tgatagtgcc ggtcgtcccc
    34741 atgctggaga caaaatgagt gatgcgcccg ccggtttgct gccagatttc cggcccggtg
    34801 gtggtgtaat gcgcataagg gttatcggga ttattgaact gatcgagcag ctttccttcg
    34861 ccacgattcg ccatctccag cgccagatcg cgcgcacctt ccatgccctg ctctttggtg
    34921 acaagaatca gttccgcacc ataagcacgc atcgccgcac ggcgttcctg gctcatgttg
    34981 tcgggcatca gcaatttcat gcgatagcct ttcagcgcgg caatcattgc cagcgcaatg
    35041 ccggtgttac cactggtggc ttcgattaag acatcacccg gtttaatttc cccgcgcttt
    35101 tccgcctcga cgatcatcga aagtgccgca cgatctttca ccgaacctgc cgggttattg
    35161 ccttccagtt ttaaccacac ttcactgccg ttatccggcc ccattcgctg caacttcacc
    35221 agaggcgtat tgcctattgt ttgttctaat gtactcacaa tctctatcca tacgtggtgt
    35281 tgcctgatgc gacgcttgcg cgtcttatca ggcctacagg ttacaaacct tgccattaaa
    35341 aagcccggat cgcgggaagc gcctccgggc gtttaacatt cactcaacct atcaggcgct
    35401 ttgtgcgaga gcaagttcct catcgcgggt ttcaatacgc tcgtcgccgt tatacagccg
    35461 cgcatgttgc agaccaacga ataaacgctc gccatgctgc ggggcatcgt cgccatgcat
    35521 cacgaccgtc agcggttcgt tgtaccaccc cagcggctgc accactaatt gggtgtagtg
    35581 acctttcggg ctggcttcca gtacctgtac cggcagcggc gaatcgagac tggtacggcg
    35641 gctgatatcc acttcccacg ggcgcaggaa gagatccacc ggcccctgat acgcaggtgt
    35701 gtagcccagc ggccagcgat gcgcgccaac atggaactgc ccgccgcgaa tggttccctg
    35761 caggcggttc acttcgccca taaattcgag tacaaaacgg gtcgctggtt cgcgccatac
    35821 ctgattcggc gcatcagcct gttcaatatt cccctgactc atcaccacta cacgatcggc
    35881 tacttcggtc gcctcttcct ggtcgtgggt cacaaacacg ctggtgaatt tcagctcttc
    35941 atgcagttga cgcagccagc gacgcagctc tttacgcacc tgcgcatcca gcgcgccgaa
    36001 cggttcatca agcagcagaa tttgcggttc tacagccagc gcgcgcgcca gcgccacacg
    36061 ctgtttctgg ccgccggaaa gctgcgccgg ataacgatcc gccagatgcg caagctggac
    36121 catttccagc aattttgtca ctttcgcttt gatggctgcg gcattcgggc gctcgcgacg
    36181 cggcagcacc gtcaggccaa aagtgatatt gtcgaatacc gtcatatggc ggaacagcgc
    36241 gtaatgctgg aacacgaaac cgactttacg atcgcgcgcg tgcaggcgac tcacgtcggt
    36301 gccgtggaag cgaatatgcc cgctggtttg atgctccagc ccggcgataa tccgcagcag
    36361 cgtggttttc ccggaaccgg acggccccag caacgcgacc atctgaccag aaggaatatc
    36421 cagtgagata tcgttcagca cctgggtgcg accaaaagac ttcttgatat tggcaatctc
    36481 aatgctcatg atgctcctcc tgctgcgcgc gtttttcctg attctccagg cgccactgca
    36541 acatactctt taaaaacagg gtgataatcg ccatcaacgt caacagcgcg gcggcggtaa
    36601 aggagccgac ggtgttgtag tcctgctcca acaattcaat ctgtaacggc agcgaaagtg
    36661 tttcgccgcg aatcgagccg gaaaccaccg acactgcacc aaactcgcca atcgcgcggg
    36721 cgttggtcag caccacgccg taaagcagcg cccagcgaat attcggcaat gtgacgcgac
    36781 ggaacatctg ccagccggac gcgccaagca aaatcgccgc ttcgtcttcc tggctgccct
    36841 ggcttaacat caccggcacc agctcgcgca ccacaaacgg acacgtcaca aagatggtga
    36901 ccagcaccat tccgggccag gagaacataa tttgcagatt atgttcatcc agccagccgc
    36961 cgagcgggcc gttggagccg tagaacagca gatacaccag accggcaacc accggcgata
    37021 cagcaaacgg aatgtccagt agcgtcagca gtaactggcg tcccgggaag ttaaagcgcg
    37081 tcaccagcca ggccagcaga atgccgaaca ccaggtttac cggtacggca atcagcgcga
    37141 tcatcaccgt cagccagatg gcgtgcagca tgtccggatc ggccagattc tgtaaaaccg
    37201 gcatcagccc cttgctgaat gcctgcacga agatgtaaat catcggcacc agcaggatga
    37261 acgccgaaac cagcatcccg atgccaatca gaaaccattt gccccagtta atcgggcgcg
    37321 cgtcataacg cttcaattgg gtaacttccg ccattaatga cctaccacac gccgaccaaa
    37381 gcgactttgc agagtgttaa ttgagaacag cagcagcaga gatgccgcga ggatcaccga
    37441 agcaatcgcg ctcgctgccg ggtaatcaaa ctcctgtaag cgcacaaaaa tcatcagcga
    37501 cgtcacttcc gtcttccacg cgatatttcc ggcgataaaa atcaccgcgc caaattcacc
    37561 aagactacgg gtaaacgaca gcgccacgcc cgccaccagc gccggagaaa gctccggcag
    37621 caccactttg cagaaactct gccagcgcgt tgcaccaagc gtttccgccg cttcttcata
    37681 ttccgggcct aactcttcca gcaccggctg cacggtacgc accacaaacg gaatgctggt
    37741 aaaggccatc gccaccgcaa taccaagcca ggtataggtg actttgatat caaacttcgc
    37801 cagccattca ccgtaaaaac cgtttacgga aaagagcgag gccagcgtta aaccggcgac
    37861 tgccgttggc agcgcaaagg gtaaatccat cagcgcatca agcagcgtgc ggcccggaaa
    37921 gcgataacgt gttaaaatcc acgccatcag cagaccgaaa acgccgttaa aaatcgatgc
    37981 cacaaacgcc gacagcagcg tcactttgta ggccgcgacc acctgcgggt tggtgatcac
    38041 ctcccagtac tgcgcccagc tcatctcaga aagctgcatc accagcgcag agagcggcag
    38101 cagcaatatc aggcacacaa acagcagact ggtgccaagg cttaaagtaa agcccggcag
    38161 cacgcgtctt gaagaaactg caaacattac ttacgccccg ccgctaacag cttgtctaac
    38221 tcgccgccgc tggtgaagtg ggttttcatc acttccggcc atgagccaaa tttgtcttcc
    38281 acgcggaaca gttcggtctg cgggaattta tctttcagtt tgtccatgac ttccgggtta
    38341 ttcacacggt agtaatagtc ggtgatgatg gtctgcgcct gtgggctgta gagccagttc
    38401 aggtaggctt ttgcggcttt ttccgtaccg ttggcctgca catttttatc gacccacgcc
    38461 accgggaatt ccgccagaat attggttttc gggatcacca cttcaaagcc ctgtgcttca
    38521 tactgtttac ggatgttgtt cacttccgat tcaaagctaa tcagcacatc gcccaagcca
    38581 cgctcggcaa aagtggtggt tgcgccacga ccgccggtat cgaacacttc aacgtttttc
    38641 aggaactggg tcataaactg ttcggttttg gctttatcgc caccgtcagc tttatccgct
    38701 gcgccccatg ccgccagata ggtataacgc gcgttacccg acgttttcgg gttcgggaaa
    38761 atcagcttca cgtcggagcg caccaggtcg ttccagtcgt ggatattctt cgggttgccc
    38821 ttacgcacca ggaagcccat ggtggagtag aacggcgagc tattattcgg caggcgcgac
    38881 tgccagtcag ccgggatcag cttgcctttg tcgtgcagga tttgtacgtc ggtcacctgg
    38941 ttataagtga caacgtctgc ttttaagccc tgcaaaatcg ccagcgcctg ttttgatgac
    39001 ccggcatgag attgttttat cgtcagtttg tcgccgccgt tatcttttgc ccattgctgt
    39061 tcaaacggcg gattcagggc ggcaaacagc tcgcgggaga cgtcataaga actgttcagc
    39121 agttccgttg cctgtacatg gcccgccagc agcagagaag cgaccagcgc gagtgagttc
    39181 tttttcagta agttaacggc cattgcgcac ccttataaat ttaatgactt tctaatagcc
    39241 atcatattta taacggacgt gaaaggagta acggttttat ataccgtttg gtgatttgga
    39301 agttaaaaag ggaataagaa cttggttcaa caggcgtaat tgttgcaggc agtttggaca
    39361 cggacagcgc ggagaaaccg gagcgtacac atagtacgtg aggatttcga gcactgcccg
    39421 gggccaaaat gacaaatgaa atagcctggt gaacttagtt caagacgaaa tcctccccac
    39481 acatgcaggg aggaaacaga ggtgaatcag ataccgacgc taaccgtctc cggcagtgtg
    39541 ctgccgccat caatcacatt ctgtgtaccg gttaaatagc tggattcatc cgatgcgagg
    39601 aaggccgcca gttcgccgac ttccagcgga tcggcgaggc gacacagcgg gattgctttt
    39661 gccatttcag tcagcaccga ttctggatct tccgggttcg actggcgggc aatgctttcc
    39721 gccattggcg tgcggacgta tcccgggcag atggcgttaa cgcgaatacc ggattgcgcg
    39781 tactccaccg ccagcgattt agtcaggcca acaatcgccg ctttcgttaa ggcatacgcc
    39841 gtttcgccag gatcggccac catatcacca gtgactgaag acatcatcac aatgcgacca
    39901 tctttgcgcg caatcatctc cggcagcacc gccttcgtga cgttccatac gcctttaata
    39961 ttgatatcaa tatggaaatc gcgatcttca tcgctcatat cgaggaaact gcccagacga
    40021 caaacgcctg cgttattcac caggatatca atgcgccctt ctttttcctt cgcgcgtttg
    40081 atagctgcgg ctaccgacgc cgggtcacgc acatcggcga caaccgccgt acagcgatga
    40141 ccacgaccac acagttcgtc cgctagcttt tcgatctcag gggagatatc cagcaagatt
    40201 aggttcgcgc catgacgtgc aaaagttctg gcaattcctt cgccaattcc ctgcaatgcg
    40261 cccgtaatca gtgctgtctt gcccgtgagt ttacccattt taatgctctc cttgttaata
    40321 cagagcgtta cactccattc cctctgaaaa tacaggataa acaaaatatt gctgcggatc
    40381 agctcactaa ttcaggaaaa tctcaatcca gtcgctgctt tatgcatcat ttcaggcgtt
    40441 gtgttagctc actgctgcgc tgaatcattt tcaatgacgc cacatcattg atgttgcaca
    40501 agaccaacaa acagtaaatc ggtaacagaa ttttgcgccg tgcgggtaga catcgacgag
    40561 ctacgccatt cggtttcacc ggaaacggta tcgagcgtga aatgcgccag ccgccgcagc
    40621 ggagagtcag ccagagaagt aatggcaatc accgttgccc cctgctttcg tgccgcttct
    40681 gcgcacaaga caatctcttt tttactgccg ctgtaagaga tagcgatttg tacatcgcct
    40741 tttttcaacg cctgggaaac ggtggcttgt acgtgcgtgt cggcttcgca ggccacgcga
    40801 tagccaattt tcatcagttt gaacgacaga tcgcgcccca ccagcgccga tccgcccagg
    40861 cccgttatct ggataaacgg tgctttactg atgacctcaa tgattttttg cagccgcgcg
    40921 tagtcgaaca acgcacaggt ttgctccagc gccagctctt tttcgcgatt cagtttgcgg
    40981 gcaatcactt ccagcgaatc atcactggta atcgaactgt gcaggtgcag cgccgtggca
    41041 ttcgttttct cccggctggc actgtattcg ccaattaacg ccatacgcaa ttcagtaaat
    41101 ccttgcgcgc cgagcttctg ggcaaatttc acaatgctcg actggctgat accgagctgt
    41161 ttcgccatct gccgtgaaga gacggatttc agctcgctca ctctggcccg cagaaagtca
    41221 gcaatttttt gctcgttccc ggtaaattca ctttccgcat tgcgtatttt cgtcaggtac
    41281 aacatcttcc actctcctgg gctggcagca aaaattccgc ttaagttctt atgacgctct
    41341 tcacactctg cgagtgtaga ctcaatgatt cctttaagtc tgtcaattcg ggatatatga
    41401 ttccatacat ctgcaattat ttgatcgtaa atagtaaggt caccaccgat gcaactagaa
    41461 aagatgatta ctgaaggctc gaacaccgcc tcggctgaaa ttgaccgcgt atcgacgctg
    41521 gaaatgtgcc ggattatcaa cgatgaagat aaaaccgtac cgcctgccgt tgatcgcgtc
    41581 ctgccggata tcgccgcggc gatcgatgtt atccacgccc aggtcagcgg cggcggtcgt
    41641 ctgatttatc tcggtgcggg aacatccggt cgtctgggga ttctggatgc cagcgaatgt
    41701 ccgcccacct acggcgtgaa accgggtctg gtggttggtt tgattgctgg aggcgaatat
    41761 gccattcagc acgcggtgga aggagctgaa gatagccggg aaggtggtgt taacgatctg
    41821 aaaaatatta atttaacggc acaggatgtg gtggttggca ttgctgccag cggtcgcacg
    41881 ccgtatgtga ttgccggact ggaatacgca cgccagctcg gctgccgcac agtgggaatt
    41941 tcctgtaatc cggggagcgc cgtttcaacc accgctgagt ttgccattac accgattgta
    42001 ggtgccgaag ttgttaccgg ttcttcgcgg atgaaagcag gtacagcgca gaaactggtg
    42061 ctcaatatgc tttccaccgg gctgatgatt aaatccggca aagtgttcgg caacctgatg
    42121 gtcgatgtgg tcgccaccaa cgaaaaactg catgtgcgcc aggtcaatat cgttaaaaac
    42181 gccaccggat gtaatgccga acaagcggaa gcggcgttaa ttgcctgcga gcgcaactgt
    42241 aaaacggcca ttgtgatggt gctgaaaaat ctcgatgccg cagaagctaa aaaacgcctg
    42301 gatcaacacg gcggctttat tcgtcaggtt ttagacaagg aataacccat ggccaaagag
    42361 atcagcagtg aacttctgaa caccattctt acccgtgtcg gcggaccggg aaatatcgcc
    42421 agttgtggta actgtatgac gcgcctgcgt ctgggtgtac atgacagttc actggttgat
    42481 cccaatatca aaacgctgga aggcgtgaag ggcgtcattt tgaccagcga ccaggtgcag
    42541 gtcgtttttg ggcctggtaa agcgcatcgt gccgcaaaag cgatgagcga gctgctgggc
    42601 gatgcaccgg tacaggatgc cgcagaaatc gccgcccaga acaaacgtca gttaaaagcc
    42661 aaacaaacct ccggcgtgca acaatttctc gccaaattcg ccaccatctt cacgccgctg
    42721 atccccggtt ttattgccgc cggtctgctg ctggggatag cgacgttaat tgccacggtg
    42781 atgcacgttc cggcagatgc tcagggcacg ctacccgacg cgctgaattt tatgaaggtg
    42841 ttcagcaaag gtttgttcac cttcctagtg attctggtgg gctataacgc cgcccaggca
    42901 tttggcggca cgggcgtcaa tggcgcaatt attgccgcac tctttttgct cggttataac
    42961 cccgctgcaa ccaccggtta ctacgccggt tttcacgatt tctttggtct gcccatcgat
    43021 ccgcgcggca atattatcgg cgtgctgatt gccgcgtggg cctgcgcccg cattgaaggc
    43081 atggtgcgcc gctttatgcc ggacgatctc gacatgctgc tgacctcgtt aatcaccctg
    43141 ctgatcaccg ctacgcttgc gtacctgatc attatgccgc tgggtggctg gttattcgaa
    43201 ggtatgtcat ggctgtttat gcacctgaac agtaatccgt tcggttgtgc ggttttagcc
    43261 gggctgttcc tgatcgccgt ggtatttggc gtgcatcagg gctttattcc tgtttacctc
    43321 gcgttaatgg acagccaggg attcaacagc ttatttccca tcctttcaat ggcaggcgcg
    43381 ggccaggtgg gcgcggcgct ggcactctac tggcgggcgc aaccgcacag tgcgctacgc
    43441 agtcaggtac gcggggcgat tattcccggc ctgctgggcg ttggtgaacc gctgatttac
    43501 ggtgtcaccc tgccccgcat gaagccgttt gttaccgcct gtttaggcgg cgcggcgggc
    43561 ggtttgttta tcggattgat agcctggtgg ggtctgccga tgggcttaaa cagcgccttt
    43621 gggccgtctg gtctggtggc gctgccgctg atgacttccg cacaaggcat ccttccggca
    43681 atggcggttt atgctggcgg gattctggtg gcatgggttt gcgggtttat tttcaccacg
    43741 ctctttggct gccgtaacgt caatctggac tgatattatg aaacggacaa tgctctatct
    43801 ttctctgctg gctgttagtt gtagcgtcag cgccgcaaaa taccctgttc tgacagaagg
    43861 tagttgatca agtatatgcg gcgttaaagc agtaataaaa aggccagtca gtaatgttac
    43921 tgcctggcct ttaatcaatg aatcagaaac gcttacagcg ccatcaactt gtccagcgac
    43981 ggtgcgaaat aatagccgcc ggttaccggt ttggtgaaac gcaacatcgc atcacgtttg
    44041 ccatcggtat cgccaaacat gctcagcagt tgctgctcaa tgttatgcag acgcgcgcag
    44101 taggcgcaga agtacagacc gtgagtgcca ctggcggtgc cgtacggcag gctctggcga
    44161 acaatcttca gccctttgcc atcttctttc agatcaacgc gggtgaggtg agaggtttcc
    44221 ggacgttcgt cgccgtcaat ctcttcgttg gcctctttgg tgcgcccgat catcatctcc
    44281 tgatcgtgaa cgctcatccg gttgagctgc ttcaggttgt gctcccaacg ctgcacaaac
    44341 acatagctgc cgccggaatc cacgccgtct ttgataaccg ccacttcgcg gcgcgtctct
    44401 tcacccgccg ggttttctgt accgtcaaca aagccgctca ggtcacgctc ttccacccaa
    44461 cggaagccgt ggatctcttc tttcacttca atgcagtcac caaaggcttc catcgccgcc
    44521 tgagcgacag agaagtttac gtcgtgacgc agagaaagaa tgtggatcaa cacatcgaac
    44581 tgggtcgtcg gcgcaaggcc tttaccgtag cccggaaaat ctttcagctc ttctgcccca
    44641 acgccgccgc tcagagcgcg ccaggtgttg ttaccaaagg caaccaccgc accaagatgc
    44701 gcgtccggga atttcgcttc aaaagttgcc agtttgtcgg caaatgtttt actggccgca
    44761 cgcagggcgt caacttcccc tttcacgttg gcttcgatcc aaatcgccgc gcggcaatgt
    44821 tctggcaaaa tgccactctg aacctgagac attgttcctc ctgaaaataa gaatgccacg
    44881 caatccgtgg cattgttgag cgctatttta cgtgtttttt cgcgacggcg gtttgttcag
    44941 acgcaaatta acgccgccag ataattttac tgactttcca gtttttcagg gtatcgtccg
    45001 aaggcattaa cccttcagga ccacgccatt ccccgctgaa ctggtagcta atatgttgac
    45061 tgccttcggc tttacattcg acggcacgat tatcatcacc atcagctttt tggcaattgc
    45121 caaatgcttt gctgtaaagg tcgctaaacg gtgtaccgat tttaacaccg gtgtcagcag
    45181 gaatatcgct atccagcacg tcaatgcgac tgatcgtgcc ttgatcgccg ttaatcacca
    45241 tcgccacgtt gtcgcctttc atcacttcaa aaaagcgcac cacgttgccg ttcgtggttt
    45301 tcattccgct gcgcaggcga taatcgccat caagcgcatc agcaatggct tgttcttgca
    45361 gtggtgtgga cgccgttaat tcacccacgc cctgctcgct cactttggtg gacgatccaa
    45421 accagttcca cgggttagcg gcagaccagt taactgacga catcgtggaa cagccggtca
    45481 gcatcaacgg catagcgcat aacattaaac gcagcgattt catgtcactt cctttggtta
    45541 ttcaataacg ttgcttggag tgcaaattca ccaaaaagtg ccgttattct ttttcttcat
    45601 taaaacaagc gcgtaaacgt cgattggtca gcagccagat taacgccaca atatccgcca
    45661 ctaccagcgc cagaccgata ccgctaacgg attcaccgtt cagccacagc cacggttgcc
    45721 agcaaagtaa aaccacctgc gccaacagca acagaaaata gagcacatgc caggtgcgag
    45781 gaaacgaagc ccgccgaccg ctcaacaaaa acgccagcac cgccggaata ccgggaatca
    45841 gccccagcca gaaattatcg tgatcgggat aaaacagatt tagcagcgca gtaccctgct
    45901 cacgcgacgc accggcaatg acaaacagca cccaggttcg cgcctgaagc aatagcacaa
    45961 gccagaagag caagggtaaa cgcaggcgtc cgtgcgcatc ataatggaca ggatggaact
    46021 cagtactctt catcttcaat caaacgctta cccagactca gcacgtcggc gtgttcatat
    46081 cccaggcgtt catacattcc gagcaccatg tcgttatctt ccggcacgtt gatctgaatt
    46141 ttcgggcagc cgcgagcaat cagctttttc tccagccggt taagcaacgc attggcaatt
    46201 ccacgcccac gaaactctgg atgcacgcca agataataag cagacccgcg atgcccgtca
    46261 taaccgccca tcaccgttcc gaccacttca ccgtttacct cagcgaccaa aaacaaactg
    46321 acgtcatggt tcatcttacg ctcgatgtcc atttccggat cgttccacgg acgcagcaag
    46381 tcgcaacgct cccaaagggt gatgacctct tcgaaatctt cctggcgaaa tacgcgtatc
    46441 tccatggtat tcgttacctt tttgcgggtt aaaaggctga ttatggcgtg aacggtcgaa
    46501 ttagccaata tctgacgaaa atcggttgaa aaagtggcat aatggggagt tgtcaactat
    46561 tgaaatgaaa agtaaaacaa ttctcaacag caaaccgtcg taacggatta cgcgatacga
    46621 tataacatct ggaactttat tattacaact caggccgtat gagcactttt aaaccactaa
    46681 aaacactcac ttcgcgccgc caggtgctga aagccggttt agctgccctg acgttgtcag
    46741 gaatgtcgca agccattgcc aaagacgaac ctttaaaaac cagcaacgga cacagcaagc
    46801 cgaaagccaa aaaatctggc ggcaaacgtg tcgttgttct cgatccaggc cacggcggaa
    46861 ttgataccgg agcgatcgga cgcaacggtt cgaaagaaaa acatgtggtg ctggcgattg
    46921 ctaaaaacgt ccgttccatt ttgcgtaatc atgggattga tgcgcgttta acgcgttctg
    46981 gcgatacgtt tatcccactt tacgatcgcg ttgaaatcgc ccataaacat ggcgcagatc
    47041 tgtttatgtc aattcatgcc gatggcttta ccaacccgaa agctgccggt gcttcggtat
    47101 ttgccctctc taaccgtggg gcaagtagcg caatggcgaa atacctgtct gaacgcgaaa
    47161 accgcgccga tgaagttgcc ggtaaaaagg cgactgacaa ggatcaccta ttgcagcaag
    47221 tactgtttga tctggtgcaa accgatacta ttaaaaacag cctgacgctt ggctcgcata
    47281 ttctgaaaaa gattaagccg gtgcataaac tgcacagccg caacaccgaa caagccgcgt
    47341 ttgtggtgtt gaaatcgccg tcggttcctt cggtgctggt ggaaacctcg tttatcacca
    47401 acccggaaga agagcggctg ttaggcacgg cggcgtttcg tcagaaaatc gccacagcga
    47461 ttgctgaagg cgtgatcagt tatttccact ggttcgacaa ccagaaagca cattcgaaaa
    47521 agcgataagt tatgaaaccc gacgcacacc aggttaaaca gtttctgctc aaccttcagg
    47581 atacgatttg tcagcagctg accgccgtcg atggcgcaga atttgtcgaa gatagttggc
    47641 agcgcgaagc tggcggcggc gggcgcagtc gggtgttgcg taatggtggt gttttcgaac
    47701 aggcgggcgt caacttttct cacgtccacg gtgaggcgat gcctgcttcc gccaccgctc
    47761 atcgcccgga acttgccggg cgcagtttcg aggcgatggg cgtttcactg gtagtgcatc
    47821 cgcataaccc gtatgttccc accagccacg cgaatgtgcg tttttttatt gccgaaaaac
    47881 cgggtgcaga gcccgtttgg tggtttggcg gcggcttcga tttaacccct ttctatggtt
    47941 ttgaagaaga cgccattcac tggcaccgca ccgcccgtga cctgtgccag ccatttggtg
    48001 aagacgttta tccccgttac aaaaagtggt gcgacgatta cttctacctc aaacatcgca
    48061 acgaacagcg cggcattggc gggctgttct ttgatgatct gaacacgcca gatttcgacc
    48121 actgttttgc ctttatgcag gcggtaggca aaggctacac cgacgcttat ttaccaattg
    48181 tagagcgacg taaagcgatg gcctacggcg agcgcgagcg caattttcag ctctaccgtc
    48241 gcggtcgtta tgtcgagttc aatctggtct gggatcgcgg cacgctgttt ggcctgcaaa
    48301 ctggcgggcg caccgagtcg atcctgatgt caatgccgcc actggtacgc tgggagtatg
    48361 attatcagcc aaaagatggc agcccagaag cggcgttaag tgagtttatt aaggtcaggg
    48421 attgggtgta actccctcac ccccattccc gcatccgctg atgcaacgtc aacgacggct
    48481 tctcggcaaa cagctgctgg taatccgtgg caaattgccc cagatgccag aatccccact
    48541 gcatggcggc gtcttttacc gttgtgcttt gcgaccacgg gcttatcagt tcgcggcgta
    48601 cgacgttcag gcgaatgcgt ttcagccacg cgtttgggcc aatgcctaaa atagcgtgaa
    48661 acgcgttttg tagcgtgcgg cgactgacat gcagttgatt acacaaatcc agcaccgtca
    48721 ccggttcgga catgttttcc agcacatatt cacgggcgcg ggaaagcaac cgacggtaac
    48781 tctgatgact gatgctttcc gccgtcacca ttggttgcgc ttcttccagc atcgccccca
    48841 tcgccattag caaattatcc cccagcactt ttcgcactgc aggctgatgg agattttccg
    48901 ggttctcgca aaacgtcgcc agcgcctgtt ggacaaagcc ccacagcgcg gctttatgct
    48961 gctctttcac ttccagcgcc gactggctac gcagcatatg taatacccga tccggattgt
    49021 gcaaaaagtt agcctgacag gtgatgacat cttcagacag cactacgccc aggatcgtat
    49081 aatcgtccgg cgtacttaat tcaaactcgg ttccaccggg gcgcgtcgct atttctgcgc
    49141 ttcccagaca ttgcgaaccg ataaatccct gctcaccgcg cgtcgccgga atgccaaacc
    49201 agaacgagtt cggccagacc aggcacgact gacgcagcgc cagaccggtg tattcacgaa
    49261 aaacctgaat atcatcgagt agaatttccg taaattcacc atgaaacttg cccggatgca
    49321 gctgatcgta aatctgctgc taggcggtaa tcgtcaaagc atgttcatag acatccgttg
    49381 tctgtcgttg atgaacatta tccacttcga ccttcggcgt gagcttcagg ttttcgggta
    49441 agggttcatg ataaagatgg tgcaaattgg ctgtacgggt ctttttcatg atgttaatgc
    49501 cgggtgttgt aggacacccg gcacctccga caggttaatg gggcttgaga cgataacgac
    49561 tactgcgttt acgtaacgtc ccggcagaaa agagctgttc gagcgcattt ctggcttttt
    49621 ccagcggcca gccaaagtgg gcggccactt ctcctgccgt cattccctga catactgatg
    49681 ttaacagcgc caacaactcg tcagcagatt ccgcgacgat aactggcgcg tcgggttcct
    49741 ttacgggttg cttcggctgg cggttaaagc cagtaaccag ccactggatg tcatcgtctg
    49801 gccgaccgat ctccttacgg ctgatgacac gcccggtacg catcgcggca gcacaacctg
    49861 cgtccagcgc cgcacgacac gccgccagat cgccttccac caccagcgtt aagcgaccag
    49921 ggtcaagcac ttcgtgactg agcagacgaa cgttagctgc tttgagcatg gcatccgctg
    49981 catctatcgc ggcgaccatg ccgtccactt ccagcaatcc cagtgcattg atcatcggca
    50041 acctccgtta cgcacgctgg attggattac gcgcgatttc cagcactgca tcggtaaagg
    50101 cgttacaggc tgctttacac gcggcctggc taccggttaa aaatgctgcc gagtagttgg
    50161 tttcagacgg tggcgggacg taggtcacca gctgtacgtc ggcagatttc aacgcggcat
    50221 caatgccgta ggtcgcttcc agcggcggtg ccaccagata cgccatcgga tcgccaagcg
    50281 tgatcccggc ggttgatgaa agataagaac cggtacgcga aaccacatgc gccaggaagg
    50341 cggtgttttc tgcgtcattg gcccactgga aagctgcgcc attttcaata tgtgcaacca
    50401 tcgcatccag accggcacgc acttccgccg ggtttggccc accaagcata atcagcactt
    50461 caccggcagt cggtgacggg ccgtgtgccg ctcccgcata cagcgagcga ccatatacca
    50521 cttcgaccat cgcttgtttg gtcgcttcgt cagccgcaat gtaggttacg tcgtcagaat
    50581 cggcagaaat cagcccgagg ctacgaatat gcggcggcaa ttttagttca cgcgcaaatt
    50641 cagcgttaac ggaggcaatc acccgcatgg cggttaccga cggtcgaatc aaatctaaag
    50701 ctggcatgat gcctccttat cgggtcatgt tgatgccgga tgctttctgc tccagcatac
    50761 gtttcgccaa atccacaatg acggcacagc agcacgaaag agaagcaaaa gtcagctacc
    50821 tggtacacta ggcagcgggt cagcaaaggg gggcaaatgc cccttttttc atgtcagata
    50881 atcagatcat tacctcaata aactaataat cacatcgcgg ttacaaccag tttgatagtg
    50941 atagctcaaa cccagacgtg gcgctggctg caagcaaaat aagccgcaaa aatgcgaccc
    51001 catgtttact agccgcaaaa atgcggtcct atttgccaga accttttatt attcaattgg
    51061 ttagtgcaag aaaaagcgtg attgctgttc aagaagtatc cagaaaggtc atgattttga
    51121 cgctgaaaaa aaataggtgg gcatttttgc ccactcacta gccgcaaatt tgcccactca
    51181 ctagccgcat ttttgcggcc caaagtaata aatacagaga aataataaat acagatcagc
    51241 gccaagattt ttcgtctttt cggcggaatc atgagactag tgcttacgtc ctgataacct
    51301 ccagagcaga ccttaacttc tgtgcagcag gactacgctc attgccgctc ggctaggtca
    51361 aatcctcacg ataaagctgt tcggattgtg accataatgt taaaggactc tttaaatcat
    51421 tccttgacga attaactagc tggcttaagc ggaatagcat gcaggataaa aacaaaacca
    51481 gcttagcagt ggggagagct tcgcttcgct cgtctcctgg ggccaatgct caatgaaacg
    51541 acgtaaggca cggagcgctc accataggta gacacataaa catctgagag cgcaccttct
    51601 atcgcgctac gcgctcaata aaaaccaacg acaccgccac actgcaagac caatggtttt
    51661 tatcgcgtat acagaccaga caggaaacag cagcaaagca gcgtatagcc aggtgcagac
    51721 agcacactca cacgacgcac agcaggctta agtgatcact caggaaactt tatccccgcc
    51781 gccgatcacc gcgaactatc atgaccgcgc caaattcgaa aggaatggcc catagaacgc
    51841 gatgaacaca cataagcgct acacttactt aacgtcgcca cagagcgatt gagagcgctt
    51901 aaaatcgatg cgatttcatt aaatttttct cactcgcctg aatggtggcg atcaggactg
    51961 atcgcaacat tgccccgcct cgatattgag cgcataatag atacatacat taagcggaaa
    52021 agagataagt ttattgtgaa aatcaggggg ttacatatca tgcgcatatc agaacaacaa
    52081 cggcagatcc tcgactatct ggcaaaaaac gaatcaatca ccgcacgaca agccgcagat
    52141 ctcgtttatg gtggcaatgt tacccgcgtc caggttgacg ccgcccgccg ctcactcatg
    52201 acgatggtta gtaatgggct aatcaggaaa caaggccgcg ctttcgtagc tgaaagacat
    52261 atcaatcacg cttatagcca ggacgttgaa gaattgcgcc gccagatcct ttgggtattc
    52321 agcaaacagg gtcaggagta tttcctttct gaacacccaa atttcgccgc caaagagtta
    52381 acagtaagcg gattgtcgtg cgcactggat tatcttgtcg gtgaaggttg cgttacttac
    52441 aagcagctat ggcacgccct gaatacgctt gtagaagatg gtttactcat tgccacgcgt
    52501 gagaacatcg aatactggat aatgccgcgt tcaggtcgcc gcaatcatgc acggtatatt
    52561 tcaggcaaca gatacagaac gccggacttt aatccgacac aggaagatcg ggaccgtgcc
    52621 gaaatgatgt gcagggaaca ggagaaaaga attatggata agttattttc gatgcagtga
    52681 acacaagcgg gcgcaatgcc cgtttttatt gccttattcc acgcctgacg aatcaatttg
    52741 atcacacctg agaaaatgca tagccagaag ttaaggccgt tatattgcga ttgggagcgt
    52801 tttaaacggt atcaatttaa ttaattctga tgtggtgatt tgcaggcaaa aaaaacccgc
    52861 cggagaagcg ggaaatagag acatatcgtt gtagttatta acagatcact ttgaggtcga
    52921 gggcggctaa ccctcgctaa tattatcccg ctattacctt gcgggtgcaa ataatctttt
    52981 attgctttga tgatttaata aatagcactt tttgctaaca aaaaccgctg tcaatatatg
    53041 gccaaaaatg tcaacagaaa aaataaggtt tataatatta agtatcacat gggtaatggg
    53101 ttcagggaat cggaatccac cgcctcacgg aaattcaaaa tttgcttctt ctttcctccg
    53161 tgggcggctt ctttttcgta tcgaggtatt tatgaaagca ataacattag aacagttcgc
    53221 agcaatggcg aatgtatcga ccagaacgct gaggcgctgg ctaaaaaaac catcaaccgg
    53281 agtccctttg ccatttaccc cacgcggtac tcgccaatta ttctgggagc acgaatgcaa
    53341 agaatggttg tacagaacag aacgataata atcacgcatc acattttgag gtaaacatca
    53401 tgaccaatat cgaaagatta gaacgctgtg ccgatattat gcgacgcaga tggatttatg
    53461 accctgaagc gggtttattg tttagccgtg aaacaaaaga cgttatcaaa ggcagcatga
    53521 ccaataaagg gcatttggtt gtaacggtac atgaaggtaa attcaaagtg acactcacct
    53581 atcagaaagc ctgctatgta tacacatacg gcgcttatga cgagacatta tatgaagtgc
    53641 accacgttaa tttgaacaaa caggataacc gcatcaagaa tattcgcctg atgccgaaag
    53701 aaaagcaccg ccggatacat aaaaacgtcc gcaaacttat tcggctgggc cacacacaat
    53761 ggcttcaacc gcaattaatt gaacaatcac tataaacaca ggagtaaata ccatgcattc
    53821 attagacagt tattttcagc gcacgacagc ccctaaatca gccgcccagg aacgccgtga
    53881 ggaattccat gagaaagtga tgcgttctgc tgattatatt gcggacaagt tcgtggaaac
    53941 ggttcgtcca cttgttgatg aagtagctga taaacttcag tctgaaatgc cggaagatat
    54001 ggaaggcact gcaaagcggc gactcatttg cgaattaagt cgtcgttttg gcgtgtcaat
    54061 aagcgctttt aaataatcga aaaaaggggg gaaagataat ggacgcattt gaggtactgg
    54121 caaataaagg cgctgaactg gtagcacagc gtgataaaac cgccaacgaa ggggaacgcg
    54181 cactgctaaa taaacagatt aaagcgatcc gtatggccca gtttaaatta atcagtaatg
    54241 aagttatcga aacaattaaa ccgcagatcc cacaaatcgt tgctgacgct attaaagccg
    54301 ccggactgga gaaacgcatt gccgggctaa aaacgaacgc agagaaaatg gagatgtaca
    54361 aacagagcgg tgctccgaat cccgatgagt acttctcacc ggatgttgag tttatggcgc
    54421 aggttgagga acgtctgggt agctgtctca ccgaggaaca gcgtcgctat tttgatggag
    54481 tggatagttc ggcaggtatt gacctgaact cgtatttcgg tcgtgaaatt gagcattttg
    54541 atgccgcccc gtctatgccg gagccagaac cagaaatggc caccgatcct gaagctgagc
    54601 aacggaaacg cgtattcaat gcgataggga ttcggtactg atcggataaa agaaagccgg
    54661 gaattacccc ggctgactta acaatgtgac caatgaacaa atcggacaag acaaacagga
    54721 taagcagtct cgaaacaatg ttcgggaact atattagcac aagtccggag aaaatcatca
    54781 tgttggcact acgcatcatg cagggcatcg ctaagaccct cgcagaacat gtcttagacc
    54841 tgaaacattc gccactgagc aaacaggcca tgaagcgcca aacgcttcgt ctgtgggctg
    54901 aatattcgct tggaactatc aataaaatca tcgacatgaa atcggggccg agcaaccaga
    54961 gcgcggagga aatggagttc atcaggcgtt taattctcat tcgcagagac attcatagcc
    55021 agttacacag cgtgggaatc gatattaatg atgggacggg cgattaattt tcggacaagt
    55081 actatgcgcg cgcgaggagc tgaacatgaa gcaagatatt gcagacaggc tggagatact
    55141 cgaaggccag cgggcagaag cgaaacaact acgtaagcag gccagaagag cgcacaggaa
    55201 caacgaagca gagttactaa ccaaatacat cagtttcacc aattactgca tctacgagtg
    55261 ttacaaggaa gatgcagagg actggctgga ttcattgcca gaacagtact gagcagaata
    55321 acaccacgtc atgttgttct gaggtgagca tatcacctct gttcaggtgg ccaaatttag
    55381 tgtgccactt cagcatcacc gttgaaatca caataacgat ttttttgtaa atatgctctc
    55441 acatatgtag gaatagacca ggttggcatt gaaacacatc taccatgacc ataataaagt
    55501 aattttttga tatgtttatg gttttatatt aattaaaata tatctaaatt tgatagtttt
    55561 aaaattgcaa aatccagatt gtgtttgtaa tgtttaatct gattgtattg agtggcgttt
    55621 attatgaaaa ataaatcagg taattcttgt tatagagaag cagtcgatat tcatagctca
    55681 cttgagcttt catttatatt gcataaatat catttgcgtg tagatgagaa gaaatctcga
    55741 aatgtattca gcgccatacg tgatgagctc atccttgatg gggattcacg acaaaactta
    55801 gctactttct gtcaaacatg gcttgatgat gaaatccgtg agttgcttaa tttagctatt
    55861 gataaaaaca tgatttataa agatgaatat ccacaaatgg cggaacctga aggtcgctgt
    55921 gtgcgtatgc tttccgattc gtggagtttc cccgattcgc gacacacctt aggttgctca
    55981 accattggtt catctgaagc tgctatgctc ggccgcttgg cgttaaaatg gcagtggtgt
    56041 aaaaaacgcg aagtacaagg taaatcaacc gaacctgatt tgcgatcctg tgcaaatatg
    56101 ctggcataaa ttctgccgtt actgtgatgt gaaattgtgt gaaattccac ttgatgatca
    56161 tcacctgata atgaatgcta aagaggtttt gaagcatgtc gatgaaaaca ctattggtgt
    56221 cgttacgact ttaggtatta cttatttttg taaagcagat tgcgttagtg tttttaagat
    56281 aagtgttaaa gttaatatta gtttttatat ttacatcgta attatatgtt tctattttaa
    56341 aaatgttcga gagtataaaa atgtctaatg ctaatatcgc acccaaaagt aagaaaatga
    56401 cagtggggac actcgctatc atgaatataa ctgctgtagt aagtctacga tttatacctt
    56461 ctgaagcaga atacggctta ggtgccattt tttactatac ctttgctgca attatgtttt
    56521 tagtacctgt tgcctttgtt gcggctgagc tagctacaac atatccacaa aaaggggggg
    56581 catttcgatg gataggagaa gcatttgggg ctcgctgggg ttttgtagga atgcttatga
    56641 cctggctaca agtcatcccc tactttccaa cagtactgac atttggtgca gtctcggttg
    56701 catttattga tgttgatatg ggggtggcag aatcaattgc cagtaataaa tggtatattt
    56761 tatttttcgt actatttgtt tactggggag ctatttgtgt tgcattacgt ggcgttggta
    56821 ttttctcgca ggtatctaag tggtgcggta ttatagggac aattattcca gctattatcc
    56881 ttgttattct tggtttctcc tatctgtggt tcagcggtaa accagcggtg atcccattag
    56941 gttggggtga tcttttccct aatatagcta acttccagaa tattgttctt gccgccagca
    57001 tatttcttgc ttatgcaggg atggaaatga atgcggtcca tgttaacgat ttagataatc
    57061 caactaaaaa atacccaatt gcaattacca tcgcttctat aggtactatt gcaatattcc
    57121 tgctcagtac tttaggggtc gcctttatta ttcctacaga taaaattagt ctaactcaaa
    57181 gtttgttggt tgcctacgat atgctgttcg aatgggctgg agtgccatgg cttggctctg
    57241 tgatggcatt tatgctagca attggcgtac tgggtggggt ggttacttgg attgcagggc
    57301 ctaatacagg tgtattagcg atcgcgaaag ctggttattt acctaagttt ttccaaaaaa
    57361 ccaatcgtca tggaatgggg caccatctaa tgtttgtaca aggtataatt gtctcagttc
    57421 tttctgttac ttttgtgata atgccttcag ttcaagcagc ttttcagatt ttatcacagt
    57481 taacagttat gctctattta gttatgtata tgctcatgtt cgctagtgct atctatctac
    57541 gacattctca acctgataca aaacgcccat atcgagcccc tgctttattt ttctgggcaa
    57601 cacttggatt cttagggtct tttctggctt ttgtattaag ttttattccg cccgagcaaa
    57661 tcccaactgg tagtccaacc agctatgttc tttatttgtt agtattagtt gccctatttt
    57721 tattaattcc aatattaatt tacgccttcc gaaaatctga ctggaaagat ccaaactctg
    57781 atttcgaacc attttcttgg gaaaaaagaa ctagtaatta aattttccat aagctacaaa
    57841 agatgaggtt aaatatgttt agtgctgaat ctccaattgg tgtaatagac tcaggtgtcg
    57901 gtggattgac atcggtaaag gaaataatta atttgcttcc tggtgaagat attatctatt
    57961 gtggagataa tatgaatgca ccttatggca accgttctgc cgatgatatc atcagtttga
    58021 ctaaaaaaat gctgactttt ttacagtcaa ggaatgtcaa actagttgct gtcgcatgta
    58081 atactatatc ctcaactttg gaatccgaag aatacagcgg atatgctaag agctttccgt
    58141 ttcctatctt atccatcatt gaacctgcag tagaagatgt gattcgccag caatataaaa
    58201 atgttggtat tatcgctacc gagtttacta ttaaaacggg gtgtcacaaa gagctaataa
    58261 aaaaattgaa ttcaacaata aatgtatttg gagaaccaag caaaaatctt gcaatgttaa
    58321 ttgaggaagg taatcttaat gctcctgcaa tcttgaatga catcaaaaaa catgttaacc
    58381 atttaatttc cctccatcca gtaaatgaaa ttattctcgg ctgtactcac tatccaattg
    58441 ttcagaattt ttttgaagca gttgcaccgg atataaaatt tattaatcct gcacatgatc
    58501 aggctatctc tattaaaaat cacttgggac agctaaattt attaaactcc tcaaatatag
    58561 gaactttgca aataaatact agtggaagta tggagatcta caaaacggta ctaggggaat
    58621 tgagcattac gaaacctcat acattcagta tcaggcagtt ttaaatttat ccggtacacc
    58681 gtaaaatccc gtccttctga actgcacccc aaaagttgga caccaactga gtaaggtgca
    58741 ttttatggac aaacccagtt acatactggg tagtgcatcc aattagtaga acatgtgttt
    58801 ttcgataaac gctccgatca ctttttcgtg gatctcaact gagcgtgaga agcagattgt
    58861 tttacgaccc aaccgcttaa tgcgggtgcg tagcgtcaga ttattacgct caatgcgttg
    58921 ggtgaatatt ttgccggtca gatgcttatt cttcggcacc tcccggccat agctgcccca
    58981 gtcatcgctg gtcagcatgc cgatgttgaa gggtgtaagc agtgccagta gctcccggca
    59041 cgtttgatcg gttcggggac caaaagtgta ggccagtaca ccgcctgttt tggtgttgta
    59101 cgcgtacccg agccaatgtt gccgggcttt actgccaacg tagctccatt gctcatcaag
    59161 ctcgcagata aacgccacat cagcatgggc aacaggcgaa gaagttattc gctttggcgc
    59221 gagtttttta aagtccggat gacggtgtta ctaccaattt tcagtgtcct ggcggtatcg
    59281 cgaaccccgg caccattaaa ggccatttca gttatcagct ctttcatacc cggcttacgt
    59341 gcttgataag tataagtgaa ctgaaacaca cggtggcagt cacggcagcg aagtctgtca
    59401 cggcctttag ggttctgacc atggcggtaa acctgagctg actgacaacg gggacaatga
    59461 atattaacgc tggtcatgag ataacctcaa aagcccgtat tatacatcag attcaactaa
    59521 ttagaggcat cacccatcca gcattataaa acatactaac aataactaaa ttataataat
    59581 cacaacttac aatcaaataa taaatgcata agattaacaa tgattttttc gggttatatt
    59641 tatctaaaaa agcagaatat tatgtaactt attattttct aagcgaaaat ttattgtgcg
    59701 gtcagactaa caaacttgcg taccacccga agaacatgcg aagatttaag tattctttct
    59761 tttttggttt ttatttttca ttttctcttt tcttctcttt ccggttctgt tctggttttg
    59821 gttttttcct ttttattttt attctttctt cagggttatg ctaaaacacc aaaacatttt
    59881 tcaccacaaa agcatccaat catcttgcca gaccaatcac cttaaccgga tacactgccg
    59941 gatataatcc cactaacacc cagacaccaa gcaccatacc tgataactac cgtattcagg
    60001 aaggaaatgc gagtgatttc accgtcaccg gcacaaccga tccgccaaaa agaggcgtac
    60061 caatgtcgat atagtccccc gcgcggacaa tcacttcgtc aatgactgcc aacgggagtt
    60121 gttgtagctg tgggcgcaac aacatgccca gagctttacc aaagtcctgc ccggccacca
    60181 ccagcagggg atgcggattc ggaaaacgcg cgacgaaatc gaccagcgcg ttgatgaccg
    60241 tcagaaccgc ggcgtaacgc acaggcagcg aggcgggaag cgccagcacg tacacgtcag
    60301 ttttgggatc aagatccagc tgaagcagcg cctgttgcca ggcactcacc agatccgttt
    60361 catcaatcgg gatcgccacc ggcaaattgc gcagcggcag ttgtacgccc tccagccaga
    60421 ttgtgctgcc agagagcgaa agggtatgtg cacccgcgcc aatcaccgtg gagcgtacgg
    60481 tttgcgccgg aaactgcaca ttcatctcac gcaggcgcgg atggtcatgc agcgccgttg
    60541 ccagaagtgg gccaatatcg gcaaaacaga acgggttggc gggctggtgg cgataacatt
    60601 cgcccacgcc tccagaaagc gtaatgattt cgggcgtaac acctgcgggc agcaaaccgg
    60661 tttgcatcaa tgcctgcgcg agcggcgaaa gcgttccgtc aatcacttcg acaatcagcg
    60721 ccgccatccg ccgggtaacc tgcaccagct gcgcgccggt cagcgaacgg gcgtcagtgc
    60781 ctgcaccgaa gcactcatcc acaatcatct gccccggttt atgagcgtaa accacgcgcc
    60841 cctggctgtc ggtttccagc aggcgaccac cgacgttgag acaggcagtg ccgctgattt
    60901 ttccggcatc gaacagggcg tagttcgcgg tgccaccgcc gatatcgata ttcagtaccc
    60961 gacacagccg ttgttcagaa agggtttgcg ccccggctcc gtgaccggcg atcacggatt
    61021 cgaggtgcgg cccggcgctg gcgacgacaa aatcgcccag cgactgtttt tccttcatca
    61081 ctttttccat cagctgtgcg accaggcttt cggtaaactg gccttccggc agctgggcaa
    61141 tgatggtttc gcggatgcgc tggctttcgg ttttgtcggc tggcacgctg gcgggcacgg
    61201 acgccgttac cgggatcgat tcatcacatt cggtgatggt aaaacccagt agatccgcca
    61261 cttcacgggc ttccggggta ataatgctgg cgcgcagaac cacggacatt gcctgttcgc
    61321 cgcgcgcgtg tgcttcacga atatcattcg ctgtgatgag ttttttcacc tgcttcctcc
    61381 tgtttggtga gagatgccag ataatccacc agttgctgca cgctttgcgg gtcgtggctg
    61441 tttagctcga aaatcggctc ttcgaaaccc gtctcaagca ataactttcg cgttgcggcg
    61501 acgtcagcat ctggcatgtc cgttttactg atgacggcga tttgccgttt gctgacgcca
    61561 atatccagca acccggcagg caaccgactt tccgggtcat ttgcgccatg gacatagatc
    61621 agcatgtcta catcctgtag cgtggtaatt aaggcgtgat accagcgggg atggctaaaa
    61681 tattcacccg gcgtatcaat atcgccctta tcattaaatt ccacggccag tgtttttctg
    61741 gcgagggtat aattcccctg caacgcatta aataacgttg tttttcctgc cccgacgcta
    61801 cccacaaacg caattcgttt catgaatgcc tcgtattaac ttttggtcat ttcacaaagc
    61861 gtataattta ataagcgacc taaaccgctg acggtttgtg ataacgcttc ttctactgcg
    61921 cccaccgagc cataaatcac caatgcaccg ctgaagcggt cgagaaaacc gatatgcaca
    61981 tcagcggctt tcagcgctaa atcaccggcg atcatcgccg tttcgccggg agttagcgtc
    62041 ataatgccaa ttgcgcccgc atccggaacg ccgattttct tcgccagttc ctcaccggga
    62101 tgcgcgatga gatgcgccag tgtgacctgt ttgccgggca caaattcctg aatgatgcgt
    62161 tctttatcca tgagtcgccg cctgtgaaaa actttcgtgt atcgtgacaa tttttcgtca
    62221 gagtaaataa caaaattcgg caagcgcgtt taaaaggtga ggtagatcaa
//
LOCUS       DAEBXN010000007        60419 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.007, whole genome shotgun sequence.
ACCESSION   DAEBXN010000007 DAEBXN010000000
VERSION     DAEBXN010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60419
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.007"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..322
                     /locus_tag="KJL99_000440"
     CDS             <1..322
                     /locus_tag="KJL99_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBD4435306.1"
                     /translation="KVGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPND
                     SNSVYKDYDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQ
                     VTYP"
     gene            435..1229
                     /gene="panB"
                     /locus_tag="KJL99_000441"
     CDS             435..1229
                     /gene="panB"
                     /locus_tag="KJL99_000441"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HBD4435307.1"
                     /translation="MKPTTIASLQKYKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGNEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1241..2092
                     /gene="panC"
                     /locus_tag="KJL99_000442"
     CDS             1241..2092
                     /gene="panC"
                     /locus_tag="KJL99_000442"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HBD4435308.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHNGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(2166..3068)
                     /gene="rpnC"
                     /locus_tag="KJL99_000443"
     CDS             complement(2166..3068)
                     /gene="rpnC"
                     /locus_tag="KJL99_000443"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HBD4435309.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEECLKGHSTDVLYSMQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEAGHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            3342..3722
                     /gene="panD"
                     /locus_tag="KJL99_000444"
     CDS             3342..3722
                     /gene="panD"
                     /locus_tag="KJL99_000444"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HBD4435310.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3726..4955)
                     /locus_tag="KJL99_000445"
     CDS             complement(3726..4955)
                     /locus_tag="KJL99_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBD4435311.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQISDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNTINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATVFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLEKMSRLVSNQPQG"
     gene            complement(5019..5459)
                     /locus_tag="KJL99_000446"
     CDS             complement(5019..5459)
                     /locus_tag="KJL99_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBD4435312.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRECIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5564..6334)
                     /locus_tag="KJL99_000447"
     CDS             complement(5564..6334)
                     /locus_tag="KJL99_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBD4435313.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6331..7257)
                     /locus_tag="KJL99_000448"
     CDS             complement(6331..7257)
                     /locus_tag="KJL99_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBD4435314.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7366..8028
                     /gene="can"
                     /locus_tag="KJL99_000449"
     CDS             7366..8028
                     /gene="can"
                     /locus_tag="KJL99_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HBD4435315.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8069..8605)
                     /gene="hpt"
                     /locus_tag="KJL99_000450"
     CDS             complement(8069..8605)
                     /gene="hpt"
                     /locus_tag="KJL99_000450"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBD4435316.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8811..11201
                     /gene="gcd"
                     /locus_tag="KJL99_000451"
     CDS             8811..11201
                     /gene="gcd"
                     /locus_tag="KJL99_000451"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HBD4435317.1"
                     /translation="MAINNTASRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQIHADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLLPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11248..12798)
                     /gene="cueO"
                     /locus_tag="KJL99_000452"
     CDS             complement(11248..12798)
                     /gene="cueO"
                     /locus_tag="KJL99_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HBD4435318.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            12964..13311
                     /locus_tag="KJL99_000453"
     CDS             12964..13311
                     /locus_tag="KJL99_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HBD4435319.1"
                     /translation="MKTFFRTVLFGSLIAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLSNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13417..14283
                     /gene="speE"
                     /locus_tag="KJL99_000454"
     CDS             13417..14283
                     /gene="speE"
                     /locus_tag="KJL99_000454"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HBD4435320.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14299..15093
                     /gene="speD"
                     /locus_tag="KJL99_000455"
     CDS             14299..15093
                     /gene="speD"
                     /locus_tag="KJL99_000455"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HBD4435321.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15131..15493)
                     /locus_tag="KJL99_000456"
     CDS             complement(15131..15493)
                     /locus_tag="KJL99_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HBD4435322.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDAEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15668..18265)
                     /gene="acnB"
                     /locus_tag="KJL99_000457"
     CDS             complement(15668..18265)
                     /gene="acnB"
                     /locus_tag="KJL99_000457"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HBD4435323.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVNVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLSSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLAEIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18620..20473
                     /locus_tag="KJL99_000458"
                     /pseudo
     CDS             18620..20473
                     /locus_tag="KJL99_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
     gene            complement(20544..21968)
                     /gene="lpdA"
                     /locus_tag="KJL99_000459"
     CDS             complement(20544..21968)
                     /gene="lpdA"
                     /locus_tag="KJL99_000459"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBD4435324.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22293..24173)
                     /gene="aceF"
                     /locus_tag="KJL99_000460"
     CDS             complement(22293..24173)
                     /gene="aceF"
                     /locus_tag="KJL99_000460"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HBD4435325.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEATPAATPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAKAEGKSEFAENDAYVHATPLIRRLAR
                     EFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKVDFSK
                     FGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAKRKLD
                     VKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVVPVFK
                     DVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVNAPEV
                     AILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSDIRRL
                     VM"
     gene            complement(24188..26851)
                     /gene="aceE"
                     /locus_tag="KJL99_000461"
     CDS             complement(24188..26851)
                     /gene="aceE"
                     /locus_tag="KJL99_000461"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HBD4435326.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKELSTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27012..27776)
                     /gene="pdhR"
                     /locus_tag="KJL99_000462"
     CDS             complement(27012..27776)
                     /gene="pdhR"
                     /locus_tag="KJL99_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HBD4435327.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMVGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28317..29690
                     /gene="aroP"
                     /locus_tag="KJL99_000463"
     CDS             28317..29690
                     /gene="aroP"
                     /locus_tag="KJL99_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HBD4435328.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29733..30587)
                     /gene="ampE"
                     /locus_tag="KJL99_000464"
     CDS             complement(29733..30587)
                     /gene="ampE"
                     /locus_tag="KJL99_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HBD4435329.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30584..31135)
                     /gene="ampD"
                     /locus_tag="KJL99_000465"
     CDS             complement(30584..31135)
                     /gene="ampD"
                     /locus_tag="KJL99_000465"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HBD4435330.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRLDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31223..32116
                     /gene="nadC"
                     /locus_tag="KJL99_000466"
     CDS             31223..32116
                     /gene="nadC"
                     /locus_tag="KJL99_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HBD4435331.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32319..32759
                     /gene="ppdD"
                     /locus_tag="KJL99_000467"
     CDS             32319..32759
                     /gene="ppdD"
                     /locus_tag="KJL99_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HBD4435332.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSSIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            32769..33916
                     /gene="tadA"
                     /locus_tag="KJL99_000468"
                     /pseudo
     CDS             32769..33916
                     /gene="tadA"
                     /locus_tag="KJL99_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
     gene            33906..35108
                     /gene="hofC"
                     /locus_tag="KJL99_000469"
     CDS             33906..35108
                     /gene="hofC"
                     /locus_tag="KJL99_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HBD4435333.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEAFPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(35143..36186)
                     /gene="guaC"
                     /locus_tag="KJL99_000470"
     CDS             complement(35143..36186)
                     /gene="guaC"
                     /locus_tag="KJL99_000470"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HBD4435334.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHFSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGQVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            complement(36348..36386)
                     /locus_tag="KJL99_000471"
     CDS             complement(36348..36386)
                     /locus_tag="KJL99_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4435335.1"
                     /translation="MIIKTLKMSARK"
     gene            36411..37031
                     /gene="coaE"
                     /locus_tag="KJL99_000472"
     CDS             36411..37031
                     /gene="coaE"
                     /locus_tag="KJL99_000472"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HBD4435336.1"
                     /translation="MGYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGENMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            37031..37774
                     /gene="zapD"
                     /locus_tag="KJL99_000473"
     CDS             37031..37774
                     /gene="zapD"
                     /locus_tag="KJL99_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HBD4435337.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELNRQQRKLQTWIGVPGVDQSRIEALIQQLKAAVSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            37784..37981
                     /gene="yacG"
                     /locus_tag="KJL99_000474"
     CDS             37784..37981
                     /gene="yacG"
                     /locus_tag="KJL99_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HBD4435338.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38081..38470)
                     /gene="mutT"
                     /locus_tag="KJL99_000475"
     CDS             complement(38081..38470)
                     /gene="mutT"
                     /locus_tag="KJL99_000475"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HBD4435339.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGVPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(38530..41235)
                     /gene="secA"
                     /locus_tag="KJL99_000476"
     CDS             complement(38530..41235)
                     /gene="secA"
                     /locus_tag="KJL99_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBD4435340.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPITEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41297..41809)
                     /gene="secM"
                     /locus_tag="KJL99_000477"
     CDS             complement(41297..41809)
                     /gene="secM"
                     /locus_tag="KJL99_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HBD4435341.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42040..42957)
                     /gene="lpxC"
                     /locus_tag="KJL99_000478"
     CDS             complement(42040..42957)
                     /gene="lpxC"
                     /locus_tag="KJL99_000478"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HBD4435342.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43058..44209)
                     /gene="ftsZ"
                     /locus_tag="KJL99_000479"
     CDS             complement(43058..44209)
                     /gene="ftsZ"
                     /locus_tag="KJL99_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HBD4435343.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44270..45532)
                     /gene="ftsA"
                     /locus_tag="KJL99_000480"
     CDS             complement(44270..45532)
                     /gene="ftsA"
                     /locus_tag="KJL99_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HBD4435344.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(45529..46359)
                     /gene="ftsQ"
                     /locus_tag="KJL99_000481"
     CDS             complement(45529..46359)
                     /gene="ftsQ"
                     /locus_tag="KJL99_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HBD4435345.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLSWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLVRFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46361..47281)
                     /gene="ddlB"
                     /locus_tag="KJL99_000482"
     CDS             complement(46361..47281)
                     /gene="ddlB"
                     /locus_tag="KJL99_000482"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBD4435346.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSIDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47274..48749)
                     /gene="murC"
                     /locus_tag="KJL99_000483"
     CDS             complement(47274..48749)
                     /gene="murC"
                     /locus_tag="KJL99_000483"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HBD4435347.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLLNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMCVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(48803..49870)
                     /gene="murG"
                     /locus_tag="KJL99_000484"
     CDS             complement(48803..49870)
                     /gene="murG"
                     /locus_tag="KJL99_000484"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBD4435348.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHYLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(49867..51111)
                     /gene="ftsW"
                     /locus_tag="KJL99_000485"
     CDS             complement(49867..51111)
                     /gene="ftsW"
                     /locus_tag="KJL99_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HBD4435349.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSAVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51111..52427)
                     /gene="murD"
                     /locus_tag="KJL99_000486"
     CDS             complement(51111..52427)
                     /gene="murD"
                     /locus_tag="KJL99_000486"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HBD4435350.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALVA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLVRYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52430..53512)
                     /gene="mraY"
                     /locus_tag="KJL99_000487"
     CDS             complement(52430..53512)
                     /gene="mraY"
                     /locus_tag="KJL99_000487"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HBD4435351.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(53506..54864)
                     /gene="murF"
                     /locus_tag="KJL99_000488"
     CDS             complement(53506..54864)
                     /gene="murF"
                     /locus_tag="KJL99_000488"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HBD4435352.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPALV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGMEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSMGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(54861..56348)
                     /gene="murE"
                     /locus_tag="KJL99_000489"
     CDS             complement(54861..56348)
                     /gene="murE"
                     /locus_tag="KJL99_000489"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HBD4435353.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFPASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGGIA"
     gene            complement(56335..58101)
                     /gene="ftsI"
                     /locus_tag="KJL99_000490"
     CDS             complement(56335..58101)
                     /gene="ftsI"
                     /locus_tag="KJL99_000490"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HBD4435354.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNSDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALTMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(58117..58482)
                     /gene="ftsL"
                     /locus_tag="KJL99_000491"
     CDS             complement(58117..58482)
                     /gene="ftsL"
                     /locus_tag="KJL99_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HBD4435355.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58479..59420)
                     /gene="rsmH"
                     /locus_tag="KJL99_000492"
     CDS             complement(58479..59420)
                     /gene="rsmH"
                     /locus_tag="KJL99_000492"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HBD4435356.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEEHF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59422..59880)
                     /gene="mraZ"
                     /locus_tag="KJL99_000493"
     CDS             complement(59422..59880)
                     /gene="mraZ"
                     /locus_tag="KJL99_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HBD4435357.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLLSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14473 a        16732 c        14849 g        14365 t
ORIGIN      
        1 aaaggttggt actgaaaacg ggcaactcct tggtaatact ctcacaggta atgatgcagc
       61 aaaaggagtc ggtgtactca tagaaggttt agcaactagt aaaaatcctc taatgacatt
      121 gaaacctaat gattcaaatt ctgtttataa agattacgac ccgagaggca aagacgatac
      181 aacaggagga gtttacccgg atcaagatac aggtataaca taccctctcc atttccaggc
      241 cacgctacaa caggatggaa ctataccaat agaagctggt gagtttaaag ccaccagtac
      301 tttccaggta acctaccctt aataagtcca ccgcaccgcc atcatctggc ggtgcggtaa
      361 ttgataaatc attgccccca ccaatgtgac tgataaaatg ccaggcaaca cgacatcatt
      421 tatcaggata cgttatgaaa ccgaccacca ttgcttcact tcagaaatat aagcaggata
      481 aaaagcgttt cgcgaccatc accgcttacg actacagctt cgcgaaacta tttgctgatg
      541 aagggcttaa cgtcatgctg gtaggcgact cgctgggcat gaccgttcag ggccacgatt
      601 ccactctgcc agtaaccgtt gctgatatcg cctaccacac tgccgccgta cgtcgcggcg
      661 caccaaactg cctgctattg gctgacctgc cgtttatggc gtatgccacg ccggaacaag
      721 cctttgaaaa cgccgcaacg gttatgcgtg ccggtgccaa tatggtcaaa attgagggcg
      781 gtgagtggct ggtcgaaacc gtaaaaatgc tgaccgaacg tgccgttcct gtatgtggtc
      841 acttaggttt aaccccacag tcagtgaata ttttcggtgg ctacaaagta caggggcgcg
      901 gcaatgaagc gagcgatcga ctactcagcg atgcattagc cttagaagcc gcgggggcac
      961 agctgctggt gctggaatgc gtgccggttg agctggcaaa acgtattacc gaagcactgg
     1021 cgatcccggt tattggtatt ggtgcaggca acgtcactga cgggcaaatc ctcgtgatgc
     1081 acgacgcctt tggtattacc ggcggtcaca ttcctaaatt cgctaaaaat ttcctcgccg
     1141 aaacgggcga catccgcgcg gctgtgcggc agtatatggc tgaagtggag tccggcgttt
     1201 atccgggcga agaacacagt ttccattaag gagtcacgtt gtgttaatta tcgaaaccct
     1261 gccgctgctg cgtcagcaaa ttcgccgcct gcgtatggaa ggcaagcgcg tggcgctggt
     1321 gcctactatg ggtaatctgc acaatggcca tatgaagctg gtcgacgaag ccaaagcccg
     1381 cgccgatgtg gtcgtcgtca gtattttcgt taacccgatg cagttcgacc gcccggaaga
     1441 tctggcacgt tatccacgga ccttgcagga ggactgcgag aagctaaaca aacgtaaagt
     1501 ggatttagtt ttcgcccctt cggtaaaaga gatctacccg aacggtactg aaacccacac
     1561 ttacgttgac gttcctggcc tttcgaccat gctggaaggt gccagccgtc cgggacattt
     1621 tcgcggcgtt tcgactattg tcagcaagct gttcaacctg gtccagccgg acatcgcctg
     1681 cttcggtgaa aaagattttc agcaactggc gttgatccgc aaaatggttg ccgatatggg
     1741 cttcgatatt gagattgtcg gtgtgccaat tatgcgcgcc aaagacggtc tggcgctaag
     1801 ttcccgtaac ggttatctga cggcggaaca acgcaaaatt gcgcctggtc tgtacaaagt
     1861 tttaagttcg attgctgaca aattgcaggc tggggaacgg gatctcgatg aaattattac
     1921 cattgcgggg caagaactga atgaaaaagg cttccgcgcc gatgatattc agattcgcga
     1981 tgccgacaca ttgctggaag tttctgaaac cagcaaacgg gcagtaattc tggtagccgc
     2041 ctggcttggc gatgctcgcc tgatcgacaa caaaatggtc gagctggcgt aatacttaac
     2101 tggcgctacg gctgatggcg ccagttatta atttacccca cgtcatctgt cataacacag
     2161 gtaacttaaa taaggttaat taccttatca atctcagcaa gaggtaaatt tgccatctct
     2221 gcaacgtctt cccgagacat tcctttactt agaagacgca gggcgaattc ttgcctttcc
     2281 tcctgtcttc cctgctgaat ccccttctca atccctttct cttcaaacca ctgcgccagc
     2341 gtcatcatag acttccctcc cgtttccctg tctctcagca caccataaaa caaatccgct
     2401 tgttcagtat gaccgcgttg cagcatatag ttttgcatgg caactaactg acttccgcta
     2461 gtgtaccctt cgtcaatcag cgtgaccagt tgctccagca ataacattaa gtcgcgctgg
     2521 cgaatatgtt tttgcagtag ttcgagaatc gcaatccgcc gatgttgcat gatttcgtca
     2581 tccggtgtga tggtgatatc caccagcggg aaaggactgt tatagacgcg tcgcgccagc
     2641 tccggcgagt aaaacatatc aaaccagcac attgagagcg gataaggtgt ggcctcgccc
     2701 tgataaaaca gtatcggcac caccagcggc agcttatcgt ggccagcctc cagatgccgg
     2761 tgcatggcag caatagaata acgcatcatg cgaaaggcca ttttcttatc cggcttgctt
     2821 tggtgttcaa tcacaacatg cagataaccg ggattgccct gcatttgcat ggaatagagc
     2881 acgtccgtgc tgtgaccttt caggcactct tcaatgaaac tccccgactc taaatgaagc
     2941 gtgttgagat cacaaagttc gcgtaattcc actggcaaat gtatctccag aaagtcgcga
     3001 gccgtctccg catgcattaa aaattgttta aataccgcgt catgcggtgt ggtactcggt
     3061 gcatccatct tccctgtctt ccgtgtggcc ttgcgtgaag gcagtatcca gtcaaggcgt
     3121 gctatcgtca gccacctctt tattgtttac cgagcagcgt tcataaaaat atcaattcgt
     3181 tacaggcgtt acatggcacg cttcggcgcg gtacgcaaac tgctgacgca aatgccatca
     3241 gatgagtaga cactcatcaa aaatcgggca atactgcgtg agaatttcct caaagcaggc
     3301 accgcctgct tcgttaacga cagggtagaa aggtagaagt tatgattcgc acgatgctgc
     3361 agggcaaact ccaccgcgtg aaagtgactc atgcggacct gcactatgaa ggttcttgcg
     3421 ccattgacca ggattttctt gacgcagccg gtattctcga aaacgaagcc atcgatatct
     3481 ggaatgtcac caacggcaag cgtttctcca cttatgccat cgcggcagaa cgcggttcga
     3541 gaattatttc tgttaacggt gcggcggccc actgcgccag cgtcggcgat attgtcatca
     3601 tcgccagctt cgttaccatg ccagatgaag aagctcgcac ctggcgaccc aatgtcgcct
     3661 attttgaagg cgacaatgaa atgaaacgta ccgcgaaagc gattccggta caggttgctt
     3721 gattgttatc cctgcggctg gttactcacc agccgcgaca tcttctccag cgaatccgtt
     3781 cttaagatat aaagtcgttt tagtaacaac ggattatccc ccggttttac tttgcctttc
     3841 atggttgtca ccgccaggtg aaatccggca tcgtttgctg ccttcacggc tttgtcatta
     3901 aagccgccaa acggatacga aagataccag acatgtggat taaattgcgc cagagcgcgg
     3961 cgtgaacgtg caaaatcaaa cagaatattg tgctcactac ggctcagtaa tatgggtcgg
     4021 cgataaccat ctacccgatg caaaaaatgg gtatgtgact ggaaatcaaa aacatcgcga
     4081 atttcgttaa gctcagaaac gctcataaat tgcagcgatt ttgggttcca cttctgcggg
     4141 tgacgtttga tgcgtgaggt aacaataaac accgtcgcct tcatgccata ttgtttcaac
     4201 acaggatacg catagcggct caccgacttg aggccatcat caaaggtaat caccaccgct
     4261 cgcgcaggga gattgatcgt attcttcacg taaccttcca gctgagccat gctcagtgtc
     4321 gcgtatcccc tgtcgcgcag ccaggccatt tggttattga aagcgcgtac cgaggtggtc
     4381 gtcgaagtat ggcgaaaacg ggtgttttct tcgtcgcgca gaatatggtg ataagttagc
     4441 accggcaggc cattatcggg ttgggcatcc agcgcgctga tataggccag tcgatcgcca
     4501 atacggatct gataccaggt ttgatttaac ctgtctttca gtttatgcaa aatcgggtag
     4561 cgcaaattgt ccgccagtac cccaaatggc gcactgccca cactcggcgc gttgtagacc
     4621 ggcgtatctt tccaggtaat taagttctga ttactcagcg gcttgttgag gtcacccaaa
     4681 ccgtcttcaa ctttttgtcg tccctgaact ggctcgagat gccctttatc gataaaccct
     4741 ttaccaaagc caaaattaaa tgcgtaataa cttgcggcag tgggctccac ggcaatgatt
     4801 tgtccggccc gaatattccc cacggtcacc atcttgtcac taatttgcgc ccagaccgca
     4861 gcattttcga tggtttgcat ataacgggca ggtaacgcgg cactgacact tgcggtaaac
     4921 agcatcagca ggagaataac agcttgttta tacatgaaaa aataaccgag ggtgaaacaa
     4981 cgcttttatt gtagcaaaag cgccatcaga atcgcgggtt aatactcata caaattatgt
     5041 ttgaggacga aaggcggatt tttttgttgt tggtgccaaa gactactcac ctccggccca
     5101 cccagttcga caatacactc acgaaactct gaactggtca tggtttcacg gcaagccatc
     5161 atctgttcaa ttaacggtaa cgtgacacca gaaatcactt cgcaacggga gtgtttgtgg
     5221 cttaataatg aagccacgcg atacggtggc gctccggcta tatcggttaa gaagatgaca
     5281 ccctcaccag agtccgcttc atgcagagca tcgcacatca tgcggctgag catattagag
     5341 cttaatccgc gccagaaatt cacggcccgg cactgaagaa gtgccccatg ttttttctcc
     5401 agcgcatcca gtatctcttg cgcccggtca tcgtgacagg taattaccca acctaacatc
     5461 gccttcctcc ttagccgaaa atcagcctac cctgaccatt gttgataaag ggtgatagcc
     5521 gtcaaaactc ctctcccccg aatccagagg agaggaaata gccttagcta cgcaaaccac
     5581 gtccacgttg gatcagcgac caacagatca aataaaacgc cacaataaag accaccagta
     5641 cgccaaaggt agtgaccagc ggcacatcat ttataccgag gaagccgtag cggaaaccac
     5701 tgatcatata aacgattggg ttcaggtgcg acagcccttg ccagaacggc ggcaacaaag
     5761 tcagtgagta aaagaccccg cccaaatacg tgagtggcgt taacacaaag gttggcacca
     5821 ggctgatgtc atcgaacgtt ttggcaaaca cgccgttcag caaacccgca agggagaata
     5881 gcaccgccgt gagcaccagg gttaaggcaa cgaataccca cgaatgcacc tgaaacggca
     5941 caaaaaacag tgaaattgcc gtcacaagaa tgccaacaaa cagaccacgc gccacgccac
     6001 cgccgacata tccggcaata atgacgtgag tcggaaccgg cgctaccagc agctcttcaa
     6061 tattacgctg gaacttggca ccaaaaaatg atgacgcgac gttagcgtag gcgttggtga
     6121 tcaccgacat catgatcagc cccggtacga tgaactgcat atagctgaag ccatgcatat
     6181 cgccaatacg cgaacctatc aggttaccga agataataaa gtaaagggtc atggtgatga
     6241 ctggcggcac cagcgtctgc acccagatac gcataaagcg atggatctct ttcgcccaga
     6301 tgctttttag cgccacccag taaagatgca tcatgcgcga tctccttgtt tttcattaac
     6361 cagtgaaaca aacagctctt ccagacggtt agctttgtta cgcatactta atacctgaat
     6421 gccctgctca cttaactgcg taaatacgct gttgatcccc tgctcacgta gcacttcaac
     6481 ttccagcgtc gcggtatcga ccagtcgata ctgatagcca tcgagcttcg gtaacggact
     6541 tttcggtgcg agatcgagaa taaaggtttc cgatttcagc ttcgccagca gcgccttcat
     6601 cgaggtattt tccaccagct caccatgttg aataatgccg atattgcggc acagcatttc
     6661 tgcttcttcc aggtagtgtg tggtaagaat gatggtggtg cctttgtcgt ttaaatcctt
     6721 caaaaagccc cacattgagc ggcgaagttc aatatccacg cctgcggtcg gttcgtcgag
     6781 aatcagtagt ttaggttcat gcattaacgc acgggcaatc attaaacggc gcttcatccc
     6841 gccagataac atacgcgcac gttcgttgcg ttttccccat aaatcgagct gcttaagata
     6901 tttttcgctg cggatgtacg cctctttgcg ctccacaccg tagtaccctg cctgattcac
     6961 cacaatttgc tgcacggttt caaacgggtt gaagttaaat tcctgcggca ccagtcccaa
     7021 ctgacgttta gcgttcacga catctttctc gagatcgtaa ccaaatacgc tgacccgccc
     7081 ggaggtttta tttaccagag agctgataat accgatagtg gtcgatttcc cggccccgtt
     7141 cggcccgaga agcgcataaa aatcacccgc ttcgacctgc aaatctattc cacgaagcgc
     7201 ctgaacgccg cctggatagt tttttttaag ctgttgaagt tccagtgcaa tggtcataaa
     7261 tttttactta ccttacgttc ttacacttta tctgtggttt aaatcgtccc ggagttgccc
     7321 tatattagcc aaacgtaatt atttggttac aggtcgttaa cctccatgaa agacatagat
     7381 acactcatca gcaacaatgc actatggtca aaaatgctgg tggaagagga tcccgggttt
     7441 tttgagaaac tggcacaagc gcaaaaaccg cgctttctat ggattggatg ttccgacagt
     7501 cgcgttcctg cagaacgttt aaccggtctt gagccgggcg aactctttgt tcaccgtaat
     7561 gttgctaacc tagtcattca caccgacctg aactgccttt ccgtggttca gtatgcagtg
     7621 gatgtactcg aagttgaaca cattattatc tgtggccact acggttgcgg cggcgtacaa
     7681 gccgccgttg aaaacccgga actggggctt atcaacaact ggctgctgca tatccgcgat
     7741 atctggttca aacatagctc attgctcggc gaaatgccgc aagagcgccg tctggatacc
     7801 ttgtgtgaac tgaacgtcat ggaacaggtg tataacctgg gccactccac cattatgcaa
     7861 tcagcgtgga aacgcgggca gaaagttacc attcacggct gggcctacgg cattcacgac
     7921 ggcttgctgc gtgatctgga tgttaccgcc accaaccgcg aaacccttga gcaacgttac
     7981 cgtcacggga tttccaacct caagctgaaa cacgccaacc acaaataaaa atgccatgcc
     8041 ggatgcaaca catccggcaa cttcacactt actcgtccag cagaatcact ttgccaatat
     8101 acggcagatg acggtaacgc tgtgcgtaat caatgccgta acccaccaca aactcatccg
     8161 ggatcgagaa accgataaat tcaaccggga cgttcacttc acgacgggac ggtttatcca
     8221 gcagcgtaca aatcgccaac gacttcggtt cgcgcaggct taagatctca cgcactttcg
     8281 acagtgtatt ccccgagtcg atgatatctt caacaatcag cacgtccttg ccacggatat
     8341 cttcatccag atctttgagg attttcacat cacgggtggt ggacatgccg ctaccgtagc
     8401 tggaggcggt cataaagtcg acttcatgag atacctgaac ttcacggcac aggtccgcca
     8461 taaacataaa tgagccacgc agcagaccca ccagcaccat atcgctgccg ctgtctttgt
     8521 aacgctcagt aatctgacga cccagttcgg cgatacgcgc tttaatctcc gcttcgggga
     8581 tcattacttc tacagtatgt ttcatatctc taaccatatg atttaaaaac aaatcacttc
     8641 gcacgggcag gttttggcac cgcaacaaag cgcaagacgc gcagtatacc agtaaaacta
     8701 ttcattcgcg gaatctgtta ataaagctcg aattgtgatg acggtcacac atgttaaaac
     8761 ctataattaa ttgctactaa aatattaatg aattgaaatg gtgtctcttt atggcaatta
     8821 acaatacagc ctcgcgacga ttactcgtca cgctaacagc cctttttgca gcgctttgcg
     8881 ggctgtatct actcattggc ggaggctggc tggtcgcgat tggcggctcc tggtactacc
     8941 ctatcgctgg ccttgtgatg ctcggcgtcg cctggatgct gtggcgcagt aaacgcgccg
     9001 cgctttggct atacgcagcc ctgctgctcg gcaccatgat ttggggcgtc tgggaagttg
     9061 gtttcgactt ctgggcgctg actccgcgca gcgatattct ggtcttcttc ggcatctggc
     9121 tgatcctgcc gtttgtctgg cgtcgcctgg tcattcctgc cagcggcgca gttgccgcac
     9181 tggtggtcgc actgctgatt agcggtggta tcctgacctg ggccggattt aacgatccgc
     9241 aggagatcaa cggcacctta agcgccgatg ccacacctgc cgaagctatc tcccccgttg
     9301 ccgatcagga ctggcctgcg tatggccgca atcaggaagg tcaacgcttt tcaccactga
     9361 agcaaattca cgccgataac gtccacaagc tgaaagaagc ctgggtgttc cgtactggcg
     9421 atgtgaagca gccgaacgat ccgggtgaaa tcaccaatga agtgacgccg attaaagtgg
     9481 gcgataccct ttacctgtgt accgctcacc agcgcctgtt tgcgcttgat gccgccagcg
     9541 gcaaagagaa atggcattac gatcctgagc tgaaaaccaa cgagtctttc cagcacgtaa
     9601 cctgccgtgg cgtctcttat catgaagcca aagcagaaac ggcttcgccg gaagtgatgg
     9661 cggattgccc gcgtcgtatc attcttccag tgaatgatgg tcgcctgatt gcgattaacg
     9721 ctgaaaacgg caaactgtgc gaaaccttcg ccaataaagg cgtgctcaat ctgcaaagca
     9781 atatgccaga caccaaaccg ggtctgtatg agccgacttc tccgccaatt atcaccgata
     9841 aaaccatcgt gatggccggt tcagtcaccg ataacttctc aacccgcgaa acgtctggcg
     9901 tgatccgtgg ttttgatgtc aacaccgggg agctgctgtg ggcttttgat ccgggcgcga
     9961 aagatccgaa cgcaatcccg tcagacgaac acacctttac ctttaactca ccaaactcgt
    10021 gggcaccagc ggcctatgac gcgaagctgg atctggtcta tctgccgatg ggcgtgacca
    10081 cgccggatat ctggggcggt aaccgcacac cggaacagga acgttatgcc agctcgattc
    10141 tggcgctgaa tgccactacc gggaaactgg cgtggagcta ccagaccgtt caccacgacc
    10201 tgtgggacat ggatcttccg gcacagccga cgctggcgga catcaccgtt aatggtcaga
    10261 aagtgccgat tatttacgct ccggcgaaaa ccggtaacat ttttgtgctc gatcgtcgta
    10321 atggcgaact ggtggttccg gccccggaaa aaccggttcc ccaaggtgct gccaaaggcg
    10381 attacgttac ccctactcaa ccattctcgg aactgagctt ccgtccgacg aaagatttga
    10441 gcggtgcgga tatgtgggga gccaccatgt ttgaccaact ggtgtgccgc gtgatgttcc
    10501 accagatgcg ctatgaaggc attttcacgc cgccatctga acagggtacg ctggtcttcc
    10561 cgggtaacct ggggatgttc gaatggggcg ggatttccgt tgatccaaat cgtgaagtgg
    10621 caattgccaa cccaatggca ctgccgtttg tttcgaaact gctcccgcgt ggtcctggca
    10681 acccgatgga gcagccgaaa gatgccaaag gcacgggtac ggaatccggc attcagccac
    10741 agtacggtgt accgtatggt gtcacgctca acccgttcct ctcaccattt ggtctgccat
    10801 gtaaacagcc agcatggggt tatatctcgg cgctggatct gaaaactaat gaagtggtgt
    10861 ggaagaaacg tattggtacg ccgcaggaca gtatgccgtt cccgatgccg gttccggtgc
    10921 cgttcaatat gggtatgccg atgctgggcg ggccaatctc cacggcgggt aacgtgctgt
    10981 ttatcgccgc tacggcagat aactacctgc gcgcttacaa catgagcaac ggtgaaaaac
    11041 tgtggcaggg tcgtttaccg gcgggtggtc aggcaacacc gatgacctat gaagtgaatg
    11101 gcaagcagta cgtggtgatc tccgcaggcg gtcacggttc atttggtacg aagatgggcg
    11161 actatattgt ggcttatgcc ctgccggatg atgtgaaata accataatgc ccggagagat
    11221 ccgggcatat ttccgaatac ggtcttttta taccgtaaac cctaacatca tccccgtatc
    11281 ttcatgctcc agcagatggc agtgcgccat ataagcatgt tctttcggtg cgttgtgatt
    11341 aaacttcacc agcacttcac tgacattgcc ttctacctta acggtatctt tccagcccgc
    11401 gcgatgagcc gctggcggtt tgccattttc tgacaagata cggaactgcg tgccgtgaat
    11461 atggaacgga tgcagcatca tgtcgcccac gccagagata acccaacgtt cgtactgccc
    11521 tttcgccgcc gcaaacatcg gcttattcat atcaaacgcc tgaccgttga ttttattggc
    11581 atggtggaaa tcgaacttcc cgccgtggtt catatgattc atattgccgt gccccatatg
    11641 gcccatcatc tggctgtgat ccattccggc catcgcctga tcgccatatt tctccattag
    11701 catctgcatc cccatcatat cgagcatcgg gtccatagaa agttgcagct tgcgtaccgt
    11761 cagcccttcc agcgaaggta acgcaggcag gctacttaat gtgtcaggca aagcaccgga
    11821 ggcactaata gcaatcggct gaatccgcat taccggatgc ggcttgtcaa acggtgcaat
    11881 cgccatcccc atctggctga ccggcagcgt caccaggtca aagggtttgt tgtcgttaac
    11941 ctccaccagc acttcaaaac gctcgcccat cagcaccggc agctcgctca ccttcaccgg
    12001 ttcaggtagc agaccaccgt cgctggcaat cacatacagc gggcgattgt cgctggtggc
    12061 gaaattgagc gagcgggcat tacagccatt gagcaaacgc aggcgcagcc aaccacgcgg
    12121 ggcagcgtgt tgtgggtaga ttgcaccgtt ggtcagcagc gtatcgccaa accagcccac
    12181 ggcggcggtc atcacatcca gttgataatc aatctgcccg tcggcgctaa atttcttatc
    12241 ctgaacgatc accggaacat catcgatacc ccactgtttt ggcagcatta atttcaggat
    12301 ctcgtcatct tcaatcacca ccagaccagc cagccccatc gccacctgtc gcccggtctt
    12361 gccatgttga tgtggatgga accagcaggt agcggcaggt tgatcaacgt tcaacgtcac
    12421 cgagcgcttg ccacctggcg gaataattcc ctgtgggccg ccgtcgacgt cacccggtac
    12481 ttccagcccg tgccagtgca acgtcgtctc ttccgccagt tggttgtaga tatcaaccgt
    12541 taccgctttg ccgcgctgta atttcaccgc cggccccagc agattgccgt tatagcccca
    12601 ggtagttgca gttttctcgc caaaggtgga ctgacctgcg ccaatagtta actgaatgcg
    12661 attacgggca tcggtcgtga gcaaatcagg gatcggtaac gttgggcgtt ccgccgcaaa
    12721 gactgcacgg ctccacagtg gcaaggctga agccacaccc agtgcgacgg aatatttcaa
    12781 gaaatcacga cgttgcatag ttatttcctt attcttaagc aggcgaaaca aaatcaaacg
    12841 ttgagcatag tccttcccct tacgggaagg tcaagccgca gacacaattt aatcgacaat
    12901 aatggtcgtc gcctcgctcg cagtgtgcta acgtttatct tctttaagcc cctggtagaa
    12961 gcaatgaaga cgtttttcag aacagtgtta ttcggcagcc tgatcgccgt ttgcgcaaac
    13021 agttacgcgc tcagcgagtc tgaagccgaa gatatggccg atttaacggc agtttttgtc
    13081 tttctgaaga acgattgtgg ttaccagaac ttatctaacg ggcaaattcg tcgcgcactg
    13141 gtctttttcg cccagcaaaa ccagtgggat ctcagtaatt acgacacctt cgacatgaaa
    13201 gctctcggtg aagacagcta ccgcgatctc agcggcattg gcattcccgt cgctaaaaaa
    13261 tgcaaagccc tggcccgcga ttccttaagc ctgcttgcct acgtcaaata atccctgata
    13321 cctttttgta gaaatattga ccgtgcatcc gcggtcaatg ttagctatta tgttgcgccc
    13381 tttttttacg ggtgttaaca aaggaggtat caacccatgg ccgaaaaaaa acagtggcat
    13441 gaaacgctac acgaccagtt tgggcagtac tttgcggtag ataacgttct gtatcatgaa
    13501 aagaccgatc accaggatct gatcattttt gagaacgctg catttggtcg cgtaatggcg
    13561 ctggatggcg tagtacaaac caccgagcgc gacgagttta tctatcatga gatgatgacc
    13621 catgttccgc tactggccca cggtcacgcg aaacatgtgc tgattatcgg cggcggcgac
    13681 ggtgccatgc tgcgtgaagt aacccgacat aaaaacgttg agtcaatcac gatggtggaa
    13741 atcgatgcgg gtgtcgtgtc gttctgccgt cagtatctgc ccaaccataa cgccggtagc
    13801 tacgacgatc cgcgctttca actagtgatc gacgatggcg tcaatttcgt taatcaaacc
    13861 agccagacct ttgatgtcat tatctccgac tgcaccgatc ctatcggtcc cggcgaaagc
    13921 ctgttcacct cagcatttta tgaaggctgc aaacgttgcc tgaatcctgg cggtatcttc
    13981 gtcgcacaaa acggcgtctg ctttttacag caggaagaag ccatcgacag ccatcgcaaa
    14041 ctcagccatt acttcagcga cgttggcttt tatcaggcgg cgatcccgac ctattacggc
    14101 ggtatcatga cttttgcatg ggcgacagat aacgacgcct tacgccatct ctcaaccgaa
    14161 attattcagg cgcgttttct cgcttctggc ctgaaatgcc gttattacaa tccggcaatc
    14221 catacggcag cttttgcctt acctcagtat ctgcaagacg cactggcttc acagccgtcc
    14281 taaggagaag ataagaaatt gaaaaaactg aaactgcatg gctttaataa tctgaccaaa
    14341 agtctgagtt tttgtattta cgatatctgc tacgccaaaa ctgccgaaga gcgcgacggt
    14401 tatattgctt atatcgatga actctataat gccaaccgtc tgaccgaaat cctgtcagaa
    14461 acctgttcca ttatcggggc caatattctt aacatcgccc gccaggatta cgaaccacag
    14521 ggtgccagcg tcactattct ggtgagtgaa gaaccggttg acccgaaact catcgacaaa
    14581 acagaacacc ccggcccact gccagaaacg gtggttgctc atctcgataa aagtcatatt
    14641 tgcgtacata cctacccgga aagtcatcct gaaggcggtt tatgtacctt ccgcgccgat
    14701 attgaagtct ctacctgcgg cgtgatttct ccgctgaagg cgctgaatta cctgatccac
    14761 cagcttgagt ccgatatcgt aaccattgat tatcgcgtgc gcggttttac tcgcgacatt
    14821 aacggtatga agcactttat cgaccatgag attaattcga ttcagaactt tatgtctgac
    14881 gatatgaagg cgctgtatga catggtggat gtaaacgtct atcaggaaaa tatcttccat
    14941 accaagatgt tgcttaaaga gttcgacctt aagcactaca tgttccacac caaaccggaa
    15001 gatttaaccg acagcgagcg ccaggaaatt accgctgcgc tgtggaaaga aatgcgcgag
    15061 atttattacg ggcgcaatat gccagctgtt taacggctct ggcggagctc ccaggctccg
    15121 ccagatttat ttacttctgc tgcacgaaat tgcggtaagc cgccacgacc tgcagaaaat
    15181 cctcaacgcc gcatagcgac aggctttctt cgtcgtagta gttcatcccc tcttccattt
    15241 catcgccagc gaattccagt tgattggcgc gaaccatcac ctcttcagcg tccatccaca
    15301 gggtgtattc atgccctgcc cgttgccagg accgttcgct acctttcagt gcgtgcgctg
    15361 cttgttccac ttcatcaagc aaggccaggt tttctttcac ctcttcatta aaccagtgcc
    15421 cgaccacttc atgccccatg gacatacgca cctttaccac tccggtaata tcgcgcagaa
    15481 attcgtaatc catagtgtgt tcctctgctc ccggcaatgc gccgaaatcg catcgcgcca
    15541 ttgatgtaat tatcgcagcc ttaccggaga aaaacagcgg aacggcgata gtttagaaat
    15601 aaaaaatgcc cgggaatctc attcaccggg cattgtgtcg tttatgcgca gcgcgtgcgc
    15661 ttacttatta caccgcagtc tggaaaatca ccccatcggc tttttcggtg tactgagaaa
    15721 gctggttgaa gttcagataa cgataagtat caacggcagt tttatctact tgcgcaacgt
    15781 aggtctggta ctcttccggc gtcggcagtt tgccaatcag cgccgcaaca gccgccagtt
    15841 ctgcagaagc caggaagaca ttcgcgccag tacccaaacg gttcgggaag ttacgggtag
    15901 aggtggaaac caccgtcgca ccgtctgcta cacgcgcctg gttacccata cacagggaac
    15961 agcccgggat ctcgatacga gccccgctct taccgaagac gctgtagtag ccttcttccg
    16021 tcagttgcgc ggcgtccata cgggttggcg gtgccaccca caggcgggtc ggcaactgac
    16081 ctttgtgcgc atccagcagt ttaccagcgg cacggaagtg accgatgtta gtcatgcaag
    16141 aaccgataaa tacttcgtcg atcttctcgc cctgtaccgc agacagcgga cgtgcgtcat
    16201 ccggatcgtt cggagcacac aggattggct ctttaatctc cgccagatcg atgtcgatca
    16261 ctgccgcgta ttccgcatct gcatcggctt ccagcagctc aggattcgcc agccattttt
    16321 ccatgccctg aatacggcgt tccagggtac gacgatcgcc gtaaccttcc gcgatcatcc
    16381 acttcagcag gacgatgttg gagctcaggt attcgatgat cggttctttg ttcagcttga
    16441 tagtacaacc agcggcagaa cgctcggcgg acgcatcggt tagctcaaag gcctgctcaa
    16501 ctttcagatc cggcagacct tcaatttcca gaatgcgacc agagaagatg tttttcttgc
    16561 ctttcttctc aacggtcagc agaccttgtt tgatcgcata cagcggaatg gcgtgtacca
    16621 gatcgcgcag ggtgatgccc ggctgcattt tgcctttgaa gcgcaccaga acggattccg
    16681 gcatatccag cggcattacg ccagttgcgg cagcaaacgc caccagacca gaacccgccg
    16741 ggaaagagat accgatcggg aaacgggtat gggagtcacc accggtaccg acggtatccg
    16801 gcagcagcat acggttcagc caggagtgaa tgacgccgtc acccggacgc agcgacacac
    16861 cgccacggtt cataatgaag tccggcagcg tgtggtgcgt gttcacgtca actggcttcg
    16921 gatacgccgc ggtgtggcag aaagactgca tcaccaggtc agccgagaag cccaggcacg
    16981 ccaggtcttt cagttcatca cgggtcatcg ggccggtggt gtcctgagag cctacagaag
    17041 tcattttcgg ttcgcagtac gcgcccggac gaatgccttt cacgccacag gcacggccta
    17101 ccattttctg cgccagcgag aagccgcgat cgctctcagc gacatctttc gcctgacgga
    17161 acacatcact gtgcggcaga ccaagtgctt cacgcgcttt ggtggtcagg ccacgcccga
    17221 taatcagcgg aatacggccg ccagcacgca cttcatcaat cagcacgtcg gttttcagtt
    17281 cgaaggtcgc cagcagttcg cctgtttcgt ggttacgcac ttcacctttg tacgggtaaa
    17341 cgtcaatcac gtcgcccatg ttcaggttag agacgttgac ttcgattggc agtgcacccg
    17401 cgtcttccat cgtgttaaag aagatgggtg caattttacc gccgaggcac aaaccaccgc
    17461 cgcgtttgtt cggcacatgt ggaatatcat cgcccataaa ccacagaacg gagttcgtgg
    17521 cggatttacg cgatgaaccc gtacccacaa cgtcaccgac gtacgccagc gggaaacctt
    17581 tctgttgcag agcttcaatc tgtttgatcg ggccaacaac acctggctgg tctggctcaa
    17641 taccttcacg ggcgtttttc agcatcgcca gcgcgtgcag tgggatatcc gggcgtgacc
    17701 acgcatccgg tgccggagaa aggtcatcgg tgttggtttc gccagtgact ttaaagacgg
    17761 taacggtcag tttttcagcc agcgccgggc gattcaggaa ccattcggca tccgcccagg
    17821 actgcattac ctgcttcgca tattcgttgc ctgctttcgc tttctcttct acgtcataga
    17881 agttatcgaa catcagcagc gtgtgagaaa gtgctttggc agcaataggt gccagtttgg
    17941 catcatccag cgcgtcgatc agcggatgaa tgttgtaacc accctgcatg gtgcccagca
    18001 gttcgatagc tttttccgga gtcagcagag gggatttagc ttcgccttta gcgatagcag
    18061 ccaggaagcc tgcttttaca taggcggctt catcgacgcc tgggggaaca cggttggtta
    18121 acagatctaa caggaattct tcttcgcccg cgggcgggtt tttcagcagc tctacaagcg
    18181 cggccatttg gtttgcatcc aggggtttgg gcgcaatccc ctcagcggca cgctcagcta
    18241 cgtgcttacg gtattcttct agcacgacgg ttctcctcgc tctcattgtc atagtgcggc
    18301 aggcgattct cttcacgctc ctgtgagaca gcagtttgta gggtaaatgc ccagtaccgc
    18361 aacgggcaga atagcaggat tttgacgacg tgttaatctg tttacaaaaa agcaacataa
    18421 aaaagtggct gaatcgttaa ggatggtcta gagattgttt ccctccgtaa aaacttcgtt
    18481 aattcgcatg gtgataatca cctttctcaa cgaatcccaa gattagtcaa aatttaaact
    18541 accgcctctt tatactcgga ttcacagcac ctgcgggtgg cagttcgccg accattgcga
    18601 tttccttgag atccgaatta tgaaaatgac tttgccgttt aaaccccatg tgctggcgct
    18661 aatttgcagt gccgggcttt gtgctgcctc tgccgggcta tatataaaaa gccgcacagt
    18721 ggaagcgcct gtagaaccgc aatcgacaca actggctgtg tctgacgctg ccgctgttac
    18781 gcttcctgca acggtttccg cacctcccgt aatacccgca gtcgtcaaat ccgcattcag
    18841 cactgcacaa atagatcaat gggtcgcacc cgtcgcgctg tatcccgacg ccctactttc
    18901 gcaggtgctg atggcatcaa cctatccgac aaacgttgct caagcagtgc aatggtcgca
    18961 cgataatcca cttaaacaag gcgatgctgc tattcaggcg gtatctgacc agccgtggga
    19021 cgccagcgtt aaatcactgg tggcctttcc acaattgatg gcattgatgg gcgaaaaccc
    19081 gcaatgggtg caaaacctgg gcgatgcttt tctggcgcag ccgcaggacg tgatggactc
    19141 ggtacagcga ttgcggcaac tggcgcaaca aaccggctcg ctgaagtcat caaccgaaca
    19201 gaaagttatt accacaacga agaaagctgt accggtaaaa cagacagtca cggcacccgt
    19261 cataccatcc aataccgttt taactgccaa ccccgtcatt acagagccgg caacaaccgt
    19321 catttccatt gagcccgcca atcctgatgt ggtctatatt cccaactaca acccaaccgt
    19381 ggtttacggg aactgggcca atactgcgta tccgccggtt tatctgccac caccagccgg
    19441 agaaccgttt gttgacagct ttgtgcgcgg attcggctat agcatgggcg ttgctaccac
    19501 gtacgcacta ttcagcagca tcgactgaga tgacgacgat catgaccatc atcatcatga
    19561 cgatgatgat tatcatcacc acgatggcgg tcatcgtgac agtaatggct ggcaacacaa
    19621 cggcgacaac atcaatatcg acgtcaacaa tttcaaccgt atcaccggtg agcatcttac
    19681 tgataagaat atggcatggc ggcacaatcc aaactaccgt aatggtgtgc cctatcatga
    19741 tcaggatatg gcaaagcggt ttcatcaaac cgatgtcaac ggcggaatga gtgccacgca
    19801 gctacctgct ccaacacgcg acagccagcg tcaggcggca gcaagtcagt ttcagcaacg
    19861 aacacacgcc gcccccgtca ttacacgaga tacccaacgt caggcagcgg cacagcggtt
    19921 taatgaagct gaacactatg ggagctatga cgacttccgc gacttcagcc gtcgccaacc
    19981 actgacccag cagcaaaagg acgccgctcg tcagcgttat cagtcggcct ctcctgagca
    20041 gcgccaggca gtccgcgaga aaatgcagac taacccgcag atccagcagc gaagggaggc
    20101 ggcgcgccag cgtattcagt ccgcctcgcc tgagcagcgc caggcggttc gcgagaaaat
    20161 gcagactaac ccgcagaacc agcagcgaag agaggcagcg cgtgagcgta ttcagtctgc
    20221 aactcccgaa cagcgccagg tgtttaagga aaaagtacag cagcgcccac tgaaccaaca
    20281 gcaacgtgat aacgcccgcc agcgtataca atcggcatca cctgaacaac gtcaggtttt
    20341 tcgggagaaa gttcaggaga gccgcccaca acgtctaaac gacagtaacc atactgtcag
    20401 gctgaataac gagcaacggt cagcagtacg cgaacgtctc tctgaacgtg gagcaagaag
    20461 actggaaagg taaattgcag acgtaaaaaa agcggcgtgg ttagccgctt ttttaattgc
    20521 cggatgttcc ggcaaacgaa aaattacttc ttcttcgctt tcgggttcgg caggtcggta
    20581 atgctacctt cgaacacttc tgccgccagg cccacagact cgtgcagagt cgggtgcgcg
    20641 tggatggtca gtgcgatgtc ttcagcatca caacccattt cgattgccag gccgatttca
    20701 cccagcagct cgccgccgtt ggtaccaaca atcgcaccac cgatcacacg gtgagattct
    20761 ttgtcgaaaa tcagcttggt cataccgtct gcgcagtcag aagcgatagc acgaccagaa
    20821 gcagcccacg ggaaggtggc ggtttcatag ctgatgcctt tctctttcgc ttctttctca
    20881 gtcagaccta cccatgcaac ttctggttcg gtataggcga tggacgggat aactttcgga
    20941 tcgaagtagt gtttcttacc ggcgataact tcagcggcaa cgtgaccttc gtgaacacct
    21001 ttgtgtgcca gcatcggctg accgacgata tcgccgatag caaagatgtg cggtacgttg
    21061 gtacgcagct gtttgtcaac gcggatgaaa ccacggtcgt caacttccac gccagctttg
    21121 cctgcgtcga ggtttttacc gttcggcaca cgaccaatcg ctaccagcac ggcgtcgtaa
    21181 cgctgcggtt cagctggtgc ttttttgcct tccatcgtca cataaatacc gtcttctttc
    21241 gcttcaacgg cggtaacttt ggtttccagc atcaggttga atttcttgct gatacgcttg
    21301 gtgaagactt taacgatgtc tttgtcagct gccgggataa cctggtcgaa catttcaacc
    21361 acgtcaatct gtgaacccag cgcgtggtaa acggtgccca tttccagacc gatgataccg
    21421 ccacccatta ccagcaggcg ttctggtact tctttcagtt ccagcgcgtc agtggagtcc
    21481 cagatacgcg gatcttcatg cggaataaac ggcagttgga tcgggcgaga acccgctgca
    21541 atgatcgcat tgtcgaagtt gatcacggtt ttgccgttct caccttcaac ttccagggtg
    21601 ttagccccgg tgaatttacc cagaccgttg accactttga ctttgcggcc tttcgccata
    21661 ccagccagac caccggtcag ctgattgatc actttctctt tccaggtacg aatcttgtca
    21721 atatcggttt tcggttcgcc gaagacgata ccgtgttcag ccagcgcttt ggcttcttcg
    21781 ataacttttg ctacgtgcag cagtgcttta gaagggatac agccgacgtt caggcaaaca
    21841 ccgccaaggg tgttgtaacg ttctacgatt acggtttcca gacctaaatc agcgcaacgg
    21901 aaggcagcgg agtaacctgc ggggcctgcc ccaagtacca cgacctgagt tttgatttca
    21961 gtactcatca tgacctctat atatttatct ccggcggtca tacccgtcgt ctttcaggcc
    22021 gcagcggcgt tagcttcacg ccctcacccc agtcacttac ttatgtaagc tcctggggat
    22081 tctggcgctt gctaccttgc tgcacccaga aatccatagg gtatgtgaag taatacccta
    22141 accaccaccg ggtcgttcta tccgtcggta ttttacaaaa ttgttaacaa tttttaaaca
    22201 acaaacggca accgatttgt ctattcgcta ataacctcaa tacattcatg agattaccag
    22261 aaaaaagccg gccgttgggc cggctctttt acttacatca ccagacggcg aatgtcagac
    22321 agcgtgttgt taatgatggt aatgaaacgg gcaccatcag caccgtcgat cacgcggtgg
    22381 tcgaaggaga gagaaatcgg cagcatcaga cgcggcacga actctttacc attccacacc
    22441 ggctccatcg cggacttgga aacgccgagg atagccactt ccggcgcgtt cacaatcggc
    22501 gcgaagtggg tagtacccag gccgccgatg ctggagatgg tgaagcaacc gccctgcatt
    22561 tcgcccgcag tcagcttacc gtcacgcgct ttcttagaaa tagtcatcag ctcgcgagac
    22621 agctcgatga tgcctttctt gttgacgtct ttgaataccg gaacaaccag accgttcggg
    22681 gtatccaccg ccacaccgat gttgatgtat ttcttcaggg tcagacgctg accgtcttcc
    22741 gacagcgaac tattgaagcg aggcatctgc tcaagagctg cagcaacggc tttcatgatg
    22801 aagacaaccg gggtgatctt cacatccagc ttacgtttcg ccgcttcttc gttctgctgt
    22861 ttacggaacg cttccagctc ggtgatatcg gttttgtcga agtgggtaac atgcgggatc
    22921 atcacccagt tacggctcag gttagcacca gaaattttct ggatacggcc cagttccact
    22981 tcttcgattt cgccaaactt gctgaagtcc accttcggcc acggcagcat gccagggata
    23041 ccaccgccag tcgccgccgg agctgcttct gcacgtttga tagcttcttt cacgtaagcc
    23101 tgaacgtctt cgcgcaggat acgaccttta cggccagtgc ccttcacttt cgccaggtta
    23161 acgccaaact cgcgtgccag acggcggatc agcggggtcg cgtgaacgta agcgtcgttt
    23221 tcagcaaatt cagatttgcc ttccgctttc gcagccggag cttcagcttt tgctgccgga
    23281 gccggcgctg ccgcttcctg tttcgcagga gctgccgcag gcgctgcgcc ttcaacttcg
    23341 aagatcataa tcagcgaacc agttttcact ttatcgccaa cgttgacttt cagttccttc
    23401 acgacgcctg caaacggagc cggaacttcc atagaagctt tatcgccttc tacggtaatc
    23461 agtgactgtt cagcggcaac tttgtcgccc actttcacca tcacttcggt cacttcaact
    23521 tcgtcaccgc cgatatccgg aacgttaact tctttcacgc cagctgctgg tgcaggggtc
    23581 gctgccggag tcgcttcctg tttagccgcc ggagctgccg cgcctgcttc acccgcgact
    23641 tcgaagacca taatcagcga gccggtagac actttgtcac ccacgttcac tttgatctct
    23701 ttcacggtac ctgcaaacgg agccggaact tccatagaag ccttgtcgcc ttctacggtg
    23761 atcagcgact gttcagcttc aactttatcg ccaactttca ccaggatttc ggtcacttca
    23821 acttcgtcgc tgccgatatc cggaacgtta acgtcttttg ccgccgcagc cgcgggtgct
    23881 gctgccggag ctgcttcttt cttctcttct gcctgagcag gtgcagcgtc tgctgcaccg
    23941 tcggcggaat cgaaaatcat aatcagtgcg ccggtctggg ttttatcgcc aacagagact
    24001 ttgatctctt taacgatacc cgcctgcgga gatggaactt ccatagaggc tttgtcgcct
    24061 tctacggtga tcagcgactg ttcggcttca actttgtcgc ccactttgac caggatctcg
    24121 gtgatttcaa cttcatcagc cccgatgtcc ggtactttga tttcgatagc cattattctt
    24181 ttacctctta cgccagacgc gggttaactt tatctgcatc gatgttgaat ttggcgattg
    24241 cgtcagcaac cactttctta tcgatttcgc cacgtttagc cagttcgccc agcgccgcaa
    24301 ccacgacata agaagcatca acttcgaagt ggtgacgcag gttctcacgg ctgtcggaac
    24361 gaccgaagcc atcagtaccc agtacgcgat agtcgtcagc cggtacgtaa gtacggacct
    24421 gctcagcgaa cagtttcata tagtcggtag atgccactgc cggcgcgtcg ttcatcacct
    24481 gagcgatata cggaacgcgc ggagtttcca gcggatgcag catgttccag cgttcacaat
    24541 cctgaccatc acgcgccagc tcggtgaagg aggtcacgct ataaacgtca gaacctacgc
    24601 cgtaatcttt cgccaggatc tctgccgctt cacggacgtg acgcaggatg gaaccggagc
    24661 ccagcagctg aactttacct ttgctacctt caatggtttc gagtttgtag atacctttac
    24721 ggataccttc ctcagcacct tccggcattg ccggcatgtg gtagttttcg ttcagcgtag
    24781 tgatgtagta gtaaacgttc tcttgttttt caccgtacat acgctccaga ccgtcatgca
    24841 taatgacagc aacttcgtaa gcgtaagccg ggtcgtaaga aatacagttc gggatagtca
    24901 gcgactgaat gtggctgtga ccatcttcgt gctgcagacc ttcaccgttc agggtggtac
    24961 gaccggaagt accgccgatc aggaagccac gcgcttgctg gtctccagcc gcccagcaca
    25021 gatcgccgat acgctggaag ccgaacatcg agtaatagat gtagaatggg atcatcggca
    25081 gattgttcgt gctgtaagag gtcgccgctg ccagccagga acaccctgcg cccagctcgt
    25141 tgatcccttc ctgcagaatc tgacctttct cgtcttcttt atagtaagca acctgctcgc
    25201 ggtcctgcgg ggtgtactgc tgaccgttcg ggctgtaaat accaatctga cggaacagac
    25261 cttccatacc gaaagtacgc gcttcgtcgg cgatgatcgg taccagacga tctttgatcg
    25321 acttgttctt cagcatcacg ttcagagcac gaacgaaagc gatagtggta gagagctctt
    25381 tgctctgctc ttccagcagc gcgccgaagt cttgcaggct cggcagctca agcttctcgg
    25441 tgaagttcgg ctgacggctt ggcagataac cgtgcagttt ctgacgctga gcgtgcagat
    25501 aggtatgctc ttcagaacct tccgggaagg tgatgtacgg cagtttttcg atatctgcat
    25561 cagacaccgg cacattgaaa cggtcgcgga tgtgacgcac gccgtccatg ttcattttct
    25621 taacctggtg cgcgatgttt ttaccttcag ccgcgtcgcc catgccgtaa cctttaatgg
    25681 tatgagcaag gattactgtc gctttgcctt tggtttcctg cgctttcttg aatgcagcgt
    25741 agattttctt cggatcgtga ccaccacggt tcagtgccca gatctgctcg tcagtccagt
    25801 ctgcaaccag tgctgcggtt tcaggatatt taccgaagaa gtgttcacga acgtacgcac
    25861 catctttcga tttgaaggtc tggtagtcgc cgtcaacggt ttcgttcatc agctggatca
    25921 gtttaccgct ggtatcttta cgcagcagtt catcccaacg gctaccccac atcactttga
    25981 tcacgttcca gccagcacct tcgaagatgc cttccagttc gttgatgatc ttgccgttac
    26041 cggtgaccgg gccgtcaaga cgctgcaggt tacagttgat aacgaaaacc aggttatcca
    26101 gtttttcacg ggtagcgatg gtgatcgcac ctttggattc cggttcgtcc atttcaccgt
    26161 cgccgaggaa cgcgtaaacg gtctgtttag aggtatcttt caggccacgg tgttccagat
    26221 atttcaggaa tttagcctgg taaatagcac caatcggacc cagacccata gatacggtcg
    26281 ggaactgcca gaattccggc atcagtttcg ggtgcggata ggaagagagg ccattgccgt
    26341 gaacttcctg acggaagtta tccagctgct cctgagtcag acgaccttcc aggaaagcac
    26401 gggcgtaaac gcccggggag atgtggccct ggaagtaaac caggtcgccg ccatcctgct
    26461 cgttgcgtgc acggaagaag tggttaaagc acacatcata aatggttgcg gaagactgga
    26521 aggacgccat gtggccgccc agttcgaggt cttttttcga cgcacgcagc accgtcatga
    26581 tggcgttcca gcggatagct gaacgaatac ggcgttccag ttccagatta cccggatact
    26641 ccggttgttc ttcaacgggg atggtgttga tgtagttgct gatacctgtg cctgcggcta
    26701 cgtttacacc gcctttgcgg gcttcagcaa gcagttggtc gatcagatac tgagcacgct
    26761 caacaccttc ttcacggatg accgattcga tcgcctggag ccagtcgcga gtttcgatcg
    26821 gatccacgtc atttgggaaa cgttctgaca tgggttattc cttatctatc taataacgtt
    26881 gagttttctg gaacctgtcc cattgaactc tcgccagaaa gctcaataag acaggttcta
    26941 cgtttagtta ccgcgctttt atatgcgctt gatttacaac atcttctgga taatttttac
    27001 cagaaaaatc actaattctt tcgttgctcc agacgacgca gagaacgctc acggcggctc
    27061 tcttcacggc ttctgtcgag caaaatttct tcgataaagg ccagatggcg atgcgatgct
    27121 tcgcgcgctt cttccggctt accgaccata atcgcttcaa atatgcgggt gcggtgacta
    27181 ctcaccagcg gcagcatctc gcgacgcgaa tagagcaatt cgaagttctg gcggacattc
    27241 tgggccaaca tcggctccat acaccttagc agatgaagca gaaccacatt gtgggccgct
    27301 tcggtgacgg caatctgata ctggagtacg gcgtttgatt ccgcgtccag atcgccagac
    27361 tgctgcgcca gctctatggc gtggtggagt tcacggatgc gttccttgtc ttcatcggta
    27421 ctacgcagcg cggcgtaata agcggcgata ccttccaggg cgtgtcgtgt ttcgagcaag
    27481 tcatactgtg attcaggatg gtcggagagc agctccacca gcggatcgct gaagctttgc
    27541 cataggctgc tctggacaaa agtgccgcca ccctggcgac gaagcaacaa gcccttcgct
    27601 tcgagacgtt gaatcgcctc acgcaaggag ggacgggaga cgtcgaactg ttttgccagt
    27661 tcgcgttccg gtgggagttt ttcgcccggg cggagagtgc cttcgaggat caaaaactcc
    27721 agttgctgct caatcacatc ggagagtttt ggttggcgga ttttgctgta ggccatgagt
    27781 tcctgtctta agccacttgc cgaagtcaat tggtcttacc aatttcatgt ctgtgacgct
    27841 aaagtaacaa agtattcacc ttatgtccat acaggttttg attgaaatca tgaaactgtg
    27901 cacattttaa caacttgaca tatataacgt ttcaaagttg taactatgca caaatgtaga
    27961 ctttacgtag gaaagggatt ttttaacgat tattaaacta taaaaatccg aattgaaccg
    28021 attcacttac caattttttg atttttaagt caataaaaac gaatttaaat tcactctgca
    28081 tattgagagg ggttgaggct gagctttaca aacggtttct ttttaagcaa ctcatcttca
    28141 accatgcata aagcgggtgc attcgctgcc gcataccatt attcttgatc tgacggaagt
    28201 ctttttgtaa caattcaaac ctccttgatg taaacaaatc aatacaacaa acggaattgc
    28261 aaacttacac acgcatcact gcgtagatca aaaaaacaat caccgcacga ggtttcatga
    28321 tggaaggtca acagcacggc gagcagctaa agcgcggcct taaaaaccgc catattcagc
    28381 ttatcgcgct gggtggcgcg atagggacag ggctattcct aggtagcgcc tccgtaatac
    28441 agtccgcagg gccagggatt atcctgggtt acgccattgc tggttttatc gcctttctga
    28501 tcatgcgtca gctgggtgaa atggtggtcg aagaacctgt cgcaggctcc tttagccact
    28561 ttgcttataa atactggggc agttttgccg gtttcgcctc tggctggaac tactgggtac
    28621 tgtacgtttt agttgccatg gcagagctga ctgccgtggg taaatacatt cagttctggt
    28681 atccggaaat cccaacctgg gtttctgccg ccgtattctt tgtggtgatt aacgccatca
    28741 acctgaccaa cgttaaagtg tttggcgaga tggagttctg gtttgccatt atcaaagtta
    28801 ttgcggtagt agcgatgatc atcttcggcg gctggctgct gttcagtggc aacggcggcc
    28861 cgcaggcgag cgttagcaac ctgtgggatc aaggtggttt cctgccgcac ggcttcaccg
    28921 gactggtgat gatgatggcg attatcatgt tctcgttcgg tggtctggaa ctggtgggga
    28981 tcaccgcagc agaagctgat aacccggagc aaagtatccc gaaagcaact aaccaggtta
    29041 tctaccgcat cctgattttc tatattggtt cgttagccgt tctgctctca ctgatgccgt
    29101 ggacccgcgt taccgccgat accagtccgt ttgtgctgat cttccacgag ttaggcgata
    29161 cctttgtggc gaatgcgctg aacatcgtgg tactgactgc ggcgctctcc gtgtacaaca
    29221 gctgcgtata ttgcaacagc cgtatgctgt ttggtctggc acaacagggg aacgcgccaa
    29281 aagcgctggc gtctgtcgat aaacgcggcg taccggttaa caccattctg gtgtctgcgc
    29341 tggttacagc attgtgcgta ttgattaact atcttgctcc ggaatccgca tttggcctgt
    29401 taatggcact ggtggtatcc gcactggtga tcaactgggc gatgatcagt ctggcgcata
    29461 tgaagttccg tcgcgccaag caggaacaag gcgtggtaac tcgcttccct gctctgcttt
    29521 atccgctggg taactggatc tgcctgctgt ttatggcggc ggtactggtg attatgctga
    29581 tgaccccagg aatggcgatt tcggtatacc tgatcccggt atggctgatc gtgttaggta
    29641 tcggctatct gtttaaagag aaaaccgcca aagccgtaaa agcgcattaa tctctctacg
    29701 ccctcacccg tacagggtga gggcaataat ctctacacca acgccccgta aatcgtcagt
    29761 agcgcaatca ccaccacgac cacgaacgag gttttcttcg ccattgaaac cgctgccttc
    29821 ggcgtctcca ccttatcgac atgcggttca cgcgccagag agaactgcgc cagacgcgtt
    29881 aatacctgat attgcgaagt atggaaatca cccagcgaag caaaccaggc cggtaacgct
    29941 ttctcaccat gaccgatcaa ggcatatacc acacccgcaa gacgaaccgg cacccaatcc
    30001 agtacatgaa gcactgcatc aatgccggac tgtaaacgat gatgcggcgt ctggtatcgc
    30061 gccagccagt attgccatgc acgcaagaac gcatacccca tcagcgtaac gggtccccag
    30121 gttcccccca caatcagcca gaacagcggt gcaagataaa aacgaaagtt aatccacagc
    30181 aatgcatttt gcagctcacg caaatactca cgttcgtcgc agcctgccgg gacgccgtga
    30241 atcatggtga gttcgccagc catcgtggca cgggcatggc tatcattacg tgaagcagct
    30301 gtcagataag catgataatg aagacgaact ttacctgcgc caatacacag caaaccaatc
    30361 agcagccaca ccagcagcgt gggaacgttg aacaatactc cctgcaatgc gcgtaacagt
    30421 aaaaaagtca cacccatcgc aataatggtc atgcctaacg tgcgcccgag agaaaaatgt
    30481 ttcacccgcc gaaagaacgc ttcaagacga tgatcaagct gccagtgctc gcccaactta
    30541 aacaggcgct cgaaaattaa caccagtaag gttgtaaata gcgtcatgtt gtctccttgc
    30601 tgaccagcgc acgaaaccgt gcccaatcaa atgcaggacc gggatcggtt ttccgatccg
    30661 gcgcaatatc acaatggccc gtcatgttat tagcgatatc gggatagcga tctatcagtg
    30721 cccgcgtaac cgccgcaagc tgttgatact gcgcatcggt atacgccaga gtatcggtgc
    30781 cttcaagctc aatcccaata gaaaaatcat tgcagcgttc acgcccctga tactgagaga
    30841 ctcccgcatg ccaggcacgt ttatcgaaag gaacatactg gactatttca ccatcacggc
    30901 gaatcaaaca gtgagcggag acgcgcaaat gggcgatctc agcaaagaaa ggatgtgcct
    30961 gcggatcaat agttccagtg aataatgcgt cgatccacgg accgccaaac tcgcctggcg
    31021 gcaggctaat attgtgcacc accagcaggg tgggtgtttc gtcatccagg cggcaatcgt
    31081 aatgtggtga gggaacgcgg cgcgcgccaa ccagccaccc ctgttctaac aacatgcagg
    31141 atctccttat atgtggtgct aatacccggt tcagagtagc atgtttctac cttatgattc
    31201 gttagctatc tggagtttta acatgccgcc tcgccgctac aaccctgaca cccgacgtga
    31261 cgagctgctg gaacgcatta atctcgatat ccccggcgcg gtggcccagg cgctgcggga
    31321 agatttaggc ggaacagtcg atgccaacaa tgacattacg gcaaaacttt taccggaaaa
    31381 ttctcgctct catgccacgg tgatcacccg cgagaatggc gtattttgtg gcaaacgctg
    31441 ggttgaagag gtgtttattc aactggcagg cgacgatgtc accataatct ggcatgtgga
    31501 tgacggcgat gtcatcaacg ccaatcaacc cttgttcgaa cttgaaggcc catcccgcgt
    31561 gctgttaacg ggcgaacgca ctgcgcttaa ttttgtgcaa accctttcag gagttgccag
    31621 taaggtacgc cactatgtcg aattactgga aggcaccaac acgcagttgt tggatacgcg
    31681 caaaacctta cctggcctgc gttcagctct gaaatacgcg gtactttgcg gcggcggagc
    31741 gaatcaccgt ctggggcttt ctgatgcctt cctgatcaaa gaaaaccata ttattgcctc
    31801 cggctcagtg cgccaggcgg tcgaaaaagc gtcctggctg catccggatg cgccagtaga
    31861 agtcgaagta gagaatctgg aagaacttga tgaggccctg aaagccggag ccgatatcat
    31921 catgctggat aacttcgaaa cagaacagat gcgcgaagcc gtcaaacgca ccaacggcaa
    31981 ggcgctactg gaagtgtctg gcaacgtcac tgacaaaaca ctgcgcgaat ttgccgaaac
    32041 gggcgtggac tttatctccg tcggtgcgct aactaaacac gtacaagcac tcgacctttc
    32101 aatgcgtttt cgctaattgc tgacagattt atccgctcca tcgcgggcgg ataatctccc
    32161 cttccccaac tttcttcgta acgcctcgca aatttcttgt ctttcgctgc aacgaaataa
    32221 aaaacctcgg aatctgcttt cccgtttcgt tttttgcccc tcgcctgtgc tgatggcgtt
    32281 tgccaaagta gcaccaacca aatcaaggag cgaaacagat ggacaagcaa cgcggtttta
    32341 cacttatcga actgatggtg gttattggca tcattgccat tttaagctcc attggtattc
    32401 ccgcttatca aaactacctg cgcaaagccg cactcaccga catgctacaa acctttgtgc
    32461 cttaccgtac cgccgtagag ttgtgcgcgc tggaacatgg tggattagat acctgcgacg
    32521 gtggcagcaa tggcattccc tcgcctacca ccacccgcta tgtttcagcc atgagtgtgg
    32581 caaagggcgt ggtgtcgctg accgggcaag aaagtctcaa tgggctaagc gtcgtcatga
    32641 cgccaggttg ggataacgca aacggcgtca ccggctgggc gcgcaactgc aatattcaaa
    32701 gtgacagcgc attgcagcaa gcctgcgaag atgtcttccg ctttgatgac gccaactaag
    32761 gagcggcaat gaatattcca cagctcacgg ccctgtgcct gcgttatcag ggagtcttgc
    32821 tggatgccag cgaagaagtg gttcatgttg cggtggtcga tgccccctca catgagttgc
    32881 tggacgcatt gcatttcgct accaccaaac gtattgagat cacctgctgg acgcgccaac
    32941 aaatggaagg tcacgccagt cgcacacaac agacattacc cgtagctgtt caggagaagc
    33001 atcagcccaa agcagagttg ctgactcgaa cgttacaatc tgcgctggaa caacgcgcgt
    33061 ctgatattca tatcgaacca gcggacaatg cctaccgcat ccgcttgcgt atcgacggcg
    33121 tattgcatcc tttaccggat gtttcaccgg atgccggagt cgcattaaca gccagattaa
    33181 aagtgctggg aaacctggat attgcggaac atcgcctgcc gcaggacggg caattcactg
    33241 tcgaactggc aggaaacacc gtctcatttc gtattgcgac cttaccatgt cggggtggtg
    33301 aaaaggtggt attaaggttg ttacagcagg tgagccaggc actggatgtt aacacgctgg
    33361 gaatgcagcc gttacaactg gcggactttg ctcatgcctt gcaacaacca cagggactgg
    33421 tgctggtaac tggccctaca ggcagtggca aaacggtcac ctggtgttgt ccacgctaca
    33481 caccaattcc accactgaaa cgctggtacg tttacagcaa atgggggtcg cccgctggat
    33541 gctatcatcg gcgcttacgc tggtaatagc ccagcgtctg gtacgtaaac tttgcccaca
    33601 ttgtcgccga cagcaagggg agcccatcca tattccagac actgtatggc cgtcgccgct
    33661 gccccactgg caggcacccg gttgtgtaca ttgctaccac ggtttttatg gtcgtaccgc
    33721 cttgtttgaa gtcctgccca ttacaccagc catacgccag cttatttccg ctaatactga
    33781 agttgaatcg ctggaaacgc acgcccgaca ggcgggtatg cgtacgcttt ttgaaaacgg
    33841 ctgcctggcc gtggagcaag gcttaaccac ctttgaagag ttaatccgcg tactggggat
    33901 gccgcatggc aagtaagcaa ctctggcgct ggcatggcat aaccggcgac ggcaatgcgc
    33961 aagatgggat gctatgggca gagagccgta ctttactgct tatggcacta cagcaacaga
    34021 tggttacccc actaagcctg aagcgaatcg ccatcaattc tgcgcagtgg cgaggagata
    34081 aaagcgcgga agtcattcat caactggcga cgctacttaa agccgggtta acgctttctg
    34141 aagggctggc tctgctggcg gaacagcatc ccagtaagca atggcaagcg ttgctgcaat
    34201 cgctggcgca cgatctcgaa cagggcattg ctttttccaa tgccttatta ccctggtcag
    34261 aggcatttcc gccgctctat caggcgatga tccgcacggg tgaactgacc ggtaagctgg
    34321 atgaatgctg ctttgaactg gcgcgtcagc aaaaatccca gcgtcagttg accgacaaag
    34381 tgaaatcagc gttgcgttat cccatcatca ttttagcgat ggcaatcatg gtggttgtgg
    34441 caatgctgca ttttgttctg ccggagtttg ccgctatcta taagaccttc aacaccccac
    34501 taccggcact aacgcagggg atcatgacgc tggcagactt tagtggcgaa tggggctggc
    34561 tgctggtgtt gttcggcttt ctgctggcga tagccaataa gttgctgatg cgccgcccga
    34621 cctggcttat cgcgcggcag aaattgctgt tacgcatccc gattatgggt tcactgatga
    34681 ggggacaaaa actcacgcag atctttacga ttctggcgct gacacaaagt gcaggcatta
    34741 cttttttaca gggcgtagag agcgtcagag aaacaatgcg ctgcccgtac tgggtgcaac
    34801 ttctgacaca aatccagcac gatatcagta acggtcatcc catctggctg gcgctaaaaa
    34861 atgccggtga gtttagcccg ctctgtttgc aattagtgag aacaggagag gcatccggct
    34921 cgctggacct catgttagac aacctcgccc atcatcatcg ggataacaca atggcgctgg
    34981 cggataacct cgcagcctta ctggaaccgg cgttgctgat cataacggga ggaattatcg
    35041 gtacgctggt ggtggcaatg tatctgccaa ttttccattt aggcgatgcg atgagtggga
    35101 tgggataacg ctggcgtgtt gctccacgcc agcgttggga gattacaggt tgttgaagat
    35161 gcggttttct tgttccagca cacgaataaa cgtggtgcgc ttggtcagct ctttcaggcg
    35221 tgaagcccca acgtatgtac aagctgaacg caggccgccc aaaatatcac gcgcggtatt
    35281 ttcaacctgg cctcgcagcg gcagcttaac ggttttacct tctgctgcgc ggtattccgc
    35341 aacgccgcca acgtgacgtt tcatcgcaga ctcggagctc atgccgtaga acagcataaa
    35401 tttctcgccg ttctcctcaa cgatgcgacc gccgctctct tcgtggcccg ccagcatgcc
    35461 gccaagcatg acgaaatcgg caccgccgcc aaaggctttc gccacatcgc ccggcgtggt
    35521 acagccaccg tcgctgacga tcattccgcc cagaccgtgc gccgcatcgg cacattcgat
    35581 taccgcagaa agctgcggat aaccgacgcc tgttttgacg cgagttgtac aaacagaacc
    35641 tgggccaatg ccaactttaa cgatatcagc accggagagg ataagctcct cacacatttc
    35701 accagtcact acgttgccag cacaaatggt tttggtcggc cacgcttcac gcgctttcgc
    35761 aacgaactgc acgaagtgtt cggaataacc attcgccaca tcaatacaaa cgaagtttaa
    35821 tgccgggttc aggtcgagaa tctgtttagt tttttcgaaa tccgcatcag acgtgccggt
    35881 agaaaccatc acatgtttca gcacatcagc ggaagaattg ttgataaacg cttgccactc
    35941 ttcgacagaa aagtgtttat gcacagcagt cagaatatca aaagaagcca gcgcagaggc
    36001 catagaaaat gtgcctacgg tgtccatatt tgcggcgata atcggcacgc cggaccagct
    36061 ctgacctgaa tgtttgaagg tgaattgacg ttccagttca acatcggaac ggcttttaag
    36121 agtggagcgt ttagggcgga tgagaacgtc tttaaaacct aacttcagat cttcttcaat
    36181 acgcatgtgc ggattcctgg ggttaatggc tataagggta agagcgcaac tccagtgacg
    36241 ctatcatacg cactaatcaa tgcggcgcaa gactgcgaaa ttgtcttttt ttacgctaca
    36301 atcccataaa tttacctggt aaatagtgag cgaacgtttc atcaatttca ctttcgcgcg
    36361 ctcattttta acgttttgat aatcatgatg aattcccggg aatataaatt atggggtata
    36421 tagttgcctt aacgggaggc attggcagtg gtaagagtac cgttgccaat gcgtttgctg
    36481 atctcggaat taacgtcatt gatgccgata ttattgcgcg tcaggtggtt gaaccaggtg
    36541 cacctgcgct acatgccatt gctgatcact ttggcgaaaa catgattgct gctgatggaa
    36601 cattgcagcg ccgggccttg cgcgagcgga tcttcgccaa cccggaagag aaaaactggc
    36661 ttaacgacct gctgcatccg ctgattcagc aagagacgca acaccagatc cagcaagcaa
    36721 cttcccccta tgtactgtgg gttgtgccat tgctggtaga aaactcactg tataaaaaag
    36781 cgaatcgagt gctggtggtg gatgtcagcc cagaaacgca acttaagcgc accatgcagc
    36841 gcgatgatgt aactcgcgaa catgtcgaac aaatccttgc tgctcaggca acgcgcgaag
    36901 cccgccttgc cgtggcagat gacgtcattg ataataacgg cgcaccggat gctatcgcat
    36961 cggatgttgc ccgcctgcac gcacactatt tgcagcttgc gtcgcagttt gtctcacagg
    37021 aaaaaccgta atgcagaccc aggtcctttt tgaacatcca ctaaatgaaa aaatgcgtac
    37081 atggctgcgc attgagtttt tgattcagca actcaccgtt aatttaccca tcgttgacca
    37141 cgctggcgcg ctgcatttct tccgtaatgt cagtgaatta ctggatgttt tcgagcgcgg
    37201 cgaagtccgc actgagctgt tgaaagaact taaccggcag caacgtaaac tccagacctg
    37261 gattggcgtg cctggcgtgg accagagccg tattgaagca ttaattcagc agttaaaagc
    37321 ggcggtgagc gtattaattt ccgcgccgcg tatcgggcaa tttctgcgtg aagatcgttt
    37381 gattgctctg gtgcgtcagc gactgagcat cccaggcggc tgttgcagct ttgatttacc
    37441 tacattgcac atttggctgc atctacccca ggcgcagcgc gacagccagg tagaaacctg
    37501 gattgccagc ctgaacccgc tcacccaggc acttaccatg gtgctggatt taattcgcca
    37561 gtcggccccc ttccgtaaac aaaccagcct gaatggtttt tatcaggata acggtggcga
    37621 tgccgacttg ctgcgcctga atctgtcgct cgattcacag ctttatccgc aaatttccgg
    37681 acataagagc cgttttgcca ttcgttttat gccgctggac actgaaaacg gacaggtacc
    37741 ggaacgtctg gatttcgaac tggcctgttg ctaaggagta aaaatgtcag aaactattac
    37801 ggtgaattgc ccaacctgcg ggaaaacggt ggtgtggggt gaaatcagcc cgtttcggcc
    37861 attttgctcc aaacgttgtc agctgatcga cctcggagaa tgggctgctg aagaaaaacg
    37921 aatccccagc agtggcgatc tttccgaaag cgatgactgg agcgaagagc caaagcagtg
    37981 acatttgtct gatgcctcac gtaggcctga taagacgcgg acagcgtcgc atcaggcatc
    38041 ttgtgcaaat gtcggatgcg gcgtgaacgc cttatccgac ctacagacgc ttaagcttcg
    38101 caattaccgg ttcattggct ggcggaaaat catcggcatt aagaccgacc agcgacatcc
    38161 actcaccggg ttgcccttct ttaccccacg gcaccccttc ccaactttcg accagccaga
    38221 accacagagt tatatgcctg tccgggaatt catattccag tttttcaaat agcgaaaaat
    38281 gttgtggggt aatcccgacc tcttcctgaa gttcacgcac caccgcctgt tccggcgttt
    38341 cgcccatttc aattttaccg ccgggaaact ccagtttatt cgccatgtgc gcatctgctg
    38401 cgcgacgtgt tataaagatt tcattgttct cgttgcgaat aatacctacc gcaatttgca
    38461 gctttttcat tgtcttaaac ctataaaaaa ggcgcagaat cctgcgcctt ttacttcaac
    38521 tgttagcttt tattgcaggc gaccatggca ctgcttgtat tttttaccag aaccgcacgg
    38581 gcaaggatcg ttacgtccta ctttgcgttc accggtttgc gccgccagtg cagctgcggc
    38641 tgcagagtcg tcatcctgat ggctaagctg ctgcatttgt gctaaacgct cggcttccat
    38701 acgacgctgt tgttccagct cctcaacctc ttcaggcata cgtacctgaa ctttgctcag
    38761 cgtactgata acttcatatt tcaacgactc cagcatcgct gcaaacatgg agaacgattc
    38821 acgtttgtat tcctgcttcg gatctttctg tgcatagcca cgcaggtgga taccctgacg
    38881 cagatagtcc atcgctgcca ggtgctcttt ccacagggag tcaagcgttt gcagcatgac
    38941 gcctttctcg aagtgacgca tcatctcagc accaaccact tcttctttac gctgatacac
    39001 ttcgatggac tgcgccagaa tgcgctcacg cagcgtctct tcatgcagtt ctggttcttt
    39061 atccagccac tcggtaattg gtaaatcgag gtcgaaatcg ttcttcagac gttcctgcag
    39121 ccccggaata tcccacattt cttccagcga ctgtggcgga atgtaggcat caatggtggc
    39181 tttgaacaca tcttcacgaa tgctgttgat ggtttcgctc acatcgctga catccagcag
    39241 ttcgttacgc tgggagtaaa tggcgcgacg ctgatcgtta gccacgtcat catattccag
    39301 cagttgctta cgaatgtcga agttacgact ttcaacttta cgctgggcgt tggcaatcgc
    39361 tttagtcacc cacgggtgtt caatggcttc gcctggcttc atacccagtt tacgcatcat
    39421 gccggatact cggtcggaag caaaaatacg catcagcgca tcttccatcg acaggtagaa
    39481 acgggaagaa ccagcatccc cctgacgacc agaacgaccg cgcaactggt tatcgatacg
    39541 acgggattcg tgacgctcgg taccgatgat atgcaggcca cctgcttcca gtaccgcatc
    39601 gtgacgtacc tgccagtcgg ctttaatttt ttcaatttgc tctgcggtcg gattttccag
    39661 cgcggcaact tctgcctgcc agctacctcc gagcacaata tctgtaccac gacccgccat
    39721 gttggtcgcg atagtcaccg cagccggata acctgcctga gcaacaatcg ccgcttcgtt
    39781 ggcgtggaat ttggcgttca ggacattgtg cttaataccg gctttggtca gttcgtttga
    39841 caccagctcc gatttttcga tggagatagt acccaccagc accggctggc ctttcgcagt
    39901 acgttctttg atatcttcaa tgatcgcctg aattttttcc gcttcagtca tgtagaccag
    39961 gtccggcaga tctttacgaa tcattggacg gttggtcgga acaacgacgg tatccagttt
    40021 gtagatggag ctaaattcga aagcttcggt atcagcagta ccggtcatcc ccgccagttt
    40081 ttcatacaga cggaagtagt tctggaaggt gatcgaagcc agcgtttggt tttcgttctg
    40141 gatctgcaca ccttctttcg cttccacagc ctggtgcaga ccatcggacc agcgtcggcc
    40201 ctgcatggta cgaccggtgt gttcgtcaac gatgataact tcaccatctt taacgatgta
    40261 gtcgacgtca cgggtaaaca gcgcatgagc gcgcagcgcc gccgttacgt ggtgcatcag
    40321 catgatgttg gccggagagt acagagactc cccttcatcc atgatgccct ctttcaccag
    40381 cagttcttca atcagcacca gaccacgttc ggtcaggttc acctggcgag atttttcgtc
    40441 caccgagaag tggccttcgc cctggaaggt ttcggagtct tctttttcct gacggatcag
    40501 gtgcggaata attttattca cgcgtttata catttccgag ctgtcttctg ccgggccgga
    40561 aatgatcagc ggtgtacgcg cttcatcgat caggatggag tccacttcgt ccaccagcgc
    40621 atagtgcagt ttacgctgta cacgttcttc agggctgaac gccatgttgt cgcgcaggta
    40681 gtcaaagccg tattcgttgt tcgtaccgta agtgatgtca gctgcgtaag cttcgcgctt
    40741 tgccggtgct ggcatgcccg gcaggttgat accaacagtc aggccaagga attcaaacag
    40801 cggacggttg ttttcggcgt cacgttgcgc caggtagtcg ttgacggtaa ctacgtgcac
    40861 gcctttaccg gttagtgcgt tcaggtaagc aggcagcgtt gcggtcaggg ttttaccttc
    40921 accggtacgc atttcggcga tgcagcgttc gttaagaacc ataccgccga gtaactgaac
    40981 gtcgaagtga cgcataccaa agacgcgctt acttgcctca cgcaccacgg cgaaagcttc
    41041 cgggatcaga ttttccagca cttcgccttt ttccagacgc gcacgaaact ctgcggtttt
    41101 ccctttcagt tcttcatcgg agagtttttc catctccggt tccatggcat tgatgatgtt
    41161 gaccactttg cgcatccggc gcagggtgcg atcgttacga ctaccgaaaa ctttagttaa
    41221 caatttgatt agcataataa aatctcaaac gccccgcgtt acggagttaa taaaatgaag
    41281 taaaggttta ttgttgttag gtgaggcgtt gagggccagc acggatgcct tgcgcctggc
    41341 ttatccagac gggcgtgctg aattttgctt gtggggtaaa atgcgcataa tcaatgcgat
    41401 aacccttttc agacggcgtg ccttcctggg tcagcagcgc gctgagcgta tccagtaatg
    41461 caagatgttg cgcctgaaga ggcaaagatt cttcagcaac gggcagtgtt tgcggtgcca
    41521 ttgcgaaaga aagatgacgg attaccgtac gaatagcatg ttgatgccag taatcaacgg
    41581 aatagttcga attcgggcgg cgcgtgttcg cttccagcaa ggccaattga ccaaagttaa
    41641 ctttggctga aggctcgtgg ttgcgggttg tcgcttttgc gggcgcgttt ggttcggcgg
    41701 cgttgctgag cgcaggcaaa cctaaactcg ccgcgaccat ccctaataag agatgcggcc
    41761 agaagtagcg tttaccaaac tgtcgccagc gcgtcagtat tccactcacg ttattgccat
    41821 cccgatcgaa ttcaaacgta tttagcgcac aaatcttatc attaaagcct gaagtccaca
    41881 gcacgaatga agataagttg tgcgtaaaag tgcttaagaa aacagcgttc gcaccgtttt
    41941 ttatagctct tataaaattc aaaaaaaaac gactggttca cctggccgga gagagtgcca
    42001 gatttgccag tcgaatttta tacgacagta taaatgtcgt tatgccagta cagctgaagg
    42061 cgctttgaag gccaacggca gttctgcgtc gtcctggaag gtcacatatt cccaggcttc
    42121 ctgtttcgcc aggacagcct gcagcagttt gttattcagt gcatgaccgg atttataagc
    42181 ggtaaatgca ccaataatat tgtgaccaca catgaacaag tcaccgatcg catcgagcat
    42241 tttgtgacgc acaaattcgt cttcaaaacg caggccgtct tcgttcagta cgcgataatc
    42301 gtcaacaacg atggcacaat cgaagctgcc gcccaggcac aaaccacggg actgcagata
    42361 ttcgatatca cgcatgaaac cgaacgtacg cgcacggctg atctggcgca taaacgcatc
    42421 agcggagaag ttcatcgcat agcgctggtt gctggaatca atagccggat ggttaaaatc
    42481 gatggtgaaa tccagcgaaa aaccattgta cggcttaaat tcagcccact tatcgccatc
    42541 ttcgacacga acagtctctt tgatgcgaac aaattttttg gcgcagttca actcgtcgat
    42601 accggcgtca agcagcaggt atacaaacgg agcggcgctg ccgtccatga tcgggatttc
    42661 cggcgcgtta acttcgataa caatgttatc gatgcccagg cccgcgagag cagcattgag
    42721 gtgctctacg gttgaaatcc gtacatcatg ctcgttgacc agacacgtac agagcatggt
    42781 atcacgcaca gatttggcat cggccgggaa atctaccggt ggattcaagt cggtgcgacg
    42841 atagatgacc ccggtgttgg ccggcgcagg gcgtaacgtc agggtgactt tcttgccggt
    42901 atgtaaaccg acacccgtcg cctgaacgat acgtttaagt gtcctttgtt tgatcatcgt
    42961 attatctcgc caaattacct atccaaccga agtgtactat acattcggcg ggccagttta
    43021 gcacaaagag cctcgaaacc caaattccag tcaattctta atcagcttgc ttacgtagga
    43081 atgctgggat atccagataa tccggctctt tcgcagtttg cggcgcattg tcattcacta
    43141 ctttagcaac cggcttctgc tcctgggtca gcggagccat cccatgctgc tggtagcgat
    43201 ccatcactgg ctgctgaacc tgcttattgg tcaccagagt gatttcagga cgtttgtcca
    43261 tgccgatacc tgtcgcaaca acggttacgc gcagctcgtc attcatatcc gggtcaagag
    43321 aagtaccgat aaccacagtc gcgttgtcgg aagcaaatgc acggatggtg ttacctaccg
    43381 tttcgaactc atccagacgc aggtcgaagc ccgccgtgat gttaaccagc acgccgcgcg
    43441 cgccagacag gtcgatatct tccagcagcg gagaagagat agccatttca gcagcttctt
    43501 ccgcacggtc ttcaccgctc gccacgccag aacccatcat tgcgtagccc atttcagaca
    43561 ttacggtgcg tacgtctgca aagtccacgt tcatcaaacc cggacgagta atcagttcgg
    43621 cgataccttg cacagcgcct ttcagtacat cgttcgctgc gccaaacgca tccagcaggg
    43681 agataccgcg gcccagaact ttcagcagtt tgtcgttcgg gatagtgatc agagagtcca
    43741 catgcttgga cagttcagtg atcccctgct cggcgaatgc catacgcttc ttgccttcaa
    43801 agttgaaagg cttagtgacg acagcaacgg tcaggatacc caaatctttt gccacttcag
    43861 cgacgactgg tgctgcacct gtaccggtac caccacccat acccgcagca ataaagacca
    43921 tgtctgcacc ttccagcgcc gcacgcaatg catcgcgatc ctcatcagcc gcattgcggc
    43981 caacttctgg attagcgcca gcgcccagtc ctttggtgat accgctaccg atttgaatcg
    44041 tctgtccaac cgctgtttta cgcagcgctt gtgcatcggt atttaccgcg aagaattcaa
    44101 caccttcaat acgctcgcgc accatgtgtt caacagcatt accgccgccg ccgccgacgc
    44161 cgatgacttt aatcaccgcg tcattggtaa gttccattgg ttcaaacata gtttctctcc
    44221 gatttgtgcc tgtcgcctga gtccgtaatc atcgtcggcc tcataaaaat taaaactctt
    44281 ttcgcagcca actattgagt cgcttgatcc acgagccaac tgatgctgta acacgttttt
    44341 ctacttcagc ttcaccgtta agatgtgact ctttcccata gtgaagcaat cccaccgccg
    44401 tcgaataata cggctcctga gcataatccg ttaaaccggt aatgttcagc ggcgcgccga
    44461 tacgcacttg cgtatgaaac acgcgctgag cacaggctgc aagaccttcg atctgcgctg
    44521 cgccaccggt taatacaatg cctgccgcca ggtgatgttt aaccccttgt tggcgaagct
    44581 tttcctgcaa ctgcaatatc tcttcgttga ccaggttgag caactcggta tagcgcggct
    44641 cgatcacctc tgccagggtc tgacgttgca gactccgcgg cggacgaccg cctacgctcg
    44701 gcacttccac gctctcatct tttccaacga tggaacccag cgcacaaccg tggcgaactt
    44761 taatcgcttc ggcgtcgctt ggcggcgtgc caaaggcgta agcgatatca ctggtcacga
    44821 cattgccagc ataaggaatt acctttgtgt ggcgcaatgc cccaccggta taaacggcga
    44881 tatccattgt accaccaccg atatcgacga cgcagacacc cagttcacgt tcatcttccg
    44941 tcaataccga ataacttgat gccagtccgg caaatatcag ttggtcaact ttcagcccac
    45001 aacgttcaac cgctttgacg atgtttttcg ccatatcgtt gtgacatgtg atcaggtgca
    45061 cttttgcctg catccgcaca cccgaaagtc ctaccggatt cttgatccct tcctgatagt
    45121 caatcgcata ctcttgcggg atcacatgca gcacacgatg ctcatcgcgc acacgcaccg
    45181 atttcgcggt atggacgacg ttttccacat cttcttgcgt cacttcttct tcagaaatag
    45241 gcaccatacc aatttcattc tggcagctga tgtgcttacc agaaagcgcc agatataccg
    45301 aagagatctg acaatctgcc atcaattctg cctggtcaat ggcgcgttgt acgcacttga
    45361 ccacggattc gaggtcgttc accccgcctt tatccatacc acgtgacggg cagctgccca
    45421 cgccaatgat attgaccata ccgtcgggca gaacttcccc tactaaagcg gcaaccttcg
    45481 cggtaccaat ttccagtcct actaccagtt ttctgtccgt cgccttgatc attgttgttc
    45541 tgcctgtgcc tgattttgtt gctgagtaga ttcctctggc ggcaagggcg cccagcctac
    45601 tgccgctcca gagtcataac gcaaatcaac gtagctaatc cgtttgccat cggtttgcgc
    45661 ctgctgctgt aaaaccggat aaagttctac aaagcgaacc aaacgtttca tcgtatcgcc
    45721 ccggccaaga ttgagcttaa tatcgttatt cagcgtcaac tgccaggaac gccgcgcggt
    45781 catcgccgct tccttcagag taaatctgtc ctttgccagc atctgcccca tttcgcgata
    45841 gccctgcaac acttcattag cgctgccttc cgggccatac agcattggaa gcacctgctt
    45901 gctggtgcga tctggcggca cgctgaaggt gtttccttcc gcgtctacca tgtgttgatc
    45961 attccaccgc gcaatcggca catattcaac cagatgaatc ttcaattcat caggccactg
    46021 ctttctgacg ctcacctgct taatccacgg caggcgttgt tctatttgcg tctggatgat
    46081 gttgacatcc tgggtcataa aggtacccgg ctcacccaat gccaggatcg actgccggat
    46141 atcgtcatta cgcgtgtaat ggcgttcacc ggtcaacacc agctttgaga gcggcaggcg
    46201 ttgcgcatct tccatccagc tcaacacgac ccagccgctc accaacactg tcgttaaaac
    46261 ggtcagcagg aaaaggatcc ccgccagacg cgttccatta ttgcggcgag aagaaacctc
    46321 ttcttcgctg tttcgcgtgt tcagagcagc ctgcgacata ttagtccgcc agttccagaa
    46381 ttcgtactac caactgcgag aagctcatac ctgcctgacg tgccgccatc ggcaccaggc
    46441 tgtggctggt catacccggt gaggtattgg cttccagcag ataaaactgt ccatcactgt
    46501 ccagcataac gtcaatacgt ccccatcctt tgcaacctaa cgtcgtccat gctttcagca
    46561 ctaatgcctg caaattggcc tcttgtgacg cttccagacc tgcggggcag aaatactgtg
    46621 tctcatcaga gagatacttc gcctcataat catagaaggt tccggacggt tgaatacgta
    46681 ttgacggtaa aatttcttca ccgagtatcg caaccgtgaa ctccggccca cttagccatt
    46741 tttcaatcaa tacttcttca tcgtgctgaa atgccaatct taatgcatct tgtagagcat
    46801 tttctgctac tacttttgac attcccacac tggaaccttc gcggctcggc ttaacgataa
    46861 ccggcaaacc cagagcagaa atttctgcta actgcttatc gctcaggcct ttttcaaact
    46921 ctgcgcgggt taacgctacc cacggcgcga ccggtaaacc ggcaccttgc cacagaagtt
    46981 tgctgcgtag tttatctatt gaaagcgcag atgccatcac tccgctgccg gtataaggca
    47041 agcccatcag ctcgagcatg ccctgcagcg taccatcttc accgccgcga ccgtgtagcg
    47101 cgataaacac tttctgaaaa cccatcgact tcagttgcgt cacgtcgact tctttcgggt
    47161 cgacaggata cgcatcaata ccgccttcac gcagcccggc taacaccgct gcgccagaat
    47221 tcagagaaac ttcccgctca gcggaagtcc cgcccaacag gaccgcgatt ttatcagtca
    47281 tgttgttctt cctccggagt ttgcggcttc agtttgattt cagctaaaga acgggcaatt
    47341 tttccgatat taccagcccc ctgaacgaga atcaggtcgt taccggttaa taccggtgcc
    47401 agcatctcgg ctacctgcgc agggtccggc accagaatgg gatcaatttt cccacgtcca
    47461 cgaattgtgc gacacagcga acggctgtcc gctcccggaa ttggcgcttc gccagccgga
    47521 tacacttcca gcatcaacag ggtatcaacc tgcgtcagca cattggcgaa atcatcatac
    47581 aggtcgcgcg tacgggtaaa acggtgcggc tgaaacagca ttaccaggtt tttatccggc
    47641 cagcctgcgc gcgccgcttt aatggtggcg tcaacttccg tcgggtggtg gccgtagtca
    47701 tcgaccagca ttgccgtacc gcttttacca ttcactggct ccagcgggaa ttcaccgagg
    47761 aaatcaaaac ggcgaccagt cccctggaag ctctccagcg cacgcaaaat agcctcgtcg
    47821 tcaattccct cttccgtagc aaccgcaacc gcagctgcgg cgttcagcgc gttatgacgg
    47881 cctggcgcat tcagggtgac gcacatcggc tctttgtcct gacgcagcag cgtaaagtgc
    47941 ccctgcgggc caatctgctg ataatcttct acacgcacgt cggcatcttc gctgaagccg
    48001 taagttgtgg tctgacgtcc cacacgcggt aacaattcgc ggatcaccgg atcatcaaca
    48061 cacatcaccg cacgaccgta aaaaggcagg ttgtgcagaa aattaataaa ggtctgtttt
    48121 aaattctcaa agtcgccctg gtaggtatcc atgtggtcgg cttcgatatt ggtgacaatc
    48181 gccaccatcg gttgcagatg caggaacgat gcatcactct catctgcttc ggcaatcagg
    48241 taccgaccat gccccaaacg cgcatgaacc cccgccgctt ttaccagccc gccgttaacg
    48301 aaggttgggt cgagccccgc ttctgcgtag atgctggaaa ccatcgcggt agttgtcgtt
    48361 ttgccgtgcg ttcctgcaat ggcgatgcca tgacgaaaac gcattaactc agccagcatt
    48421 tcggcacgac ggatcaccgg aatacgcgct tcatgagctg cgacaatttc cgggttgtcg
    48481 gcagaaatgg cgctggaaac aacgaccacg ctggcatcac gtacgttttc cgggcgatgg
    48541 ttgaaataaa tcgtcgcacc cagattcagt aactgctgcg tgaccggatt tggcgctaaa
    48601 tcggaaccac tgatctgata accttcattg gccagaactt cggcaatacc gcccataccg
    48661 gcaccaccaa tgccgacaaa atgtatgtgc cgaacgcgac gcatttcggg cacgatggaa
    48721 cgcagttttg ccaattgttg tgtattcatt ctttacgcca ttaacttctt aaattcatac
    48781 gatgcaaaag gcatcgctac aattacgccc gggcagcccg gctcacttca tttgccactc
    48841 gctcggtggc atccggaatg gatgcagcgc gggcgcgttc tgccatggtt aataaggttt
    48901 ctcgcgacca cccggccagg gtgttggcga cagcatccac gctaagctgt ggctgctcga
    48961 taattttggc tgcgcccgct ttttccagcg gtagcgcatt ccagtattgc tggcggtctt
    49021 tatgttgaaa cggcacaaac aacgccggta gtcctgccgc ggcgatttca ctcacagtta
    49081 aagcaccgga gcggcaaacg acgacatccg cccacgcata cgccgccgcc atatcatcaa
    49141 taaattcagt cactttatgc tgcggttgcc ccgcttcggc atacgcctgt tcaacggatt
    49201 gttgcgaacc tttgccgctc tgatgccaga tagtgactga atcacccagt ttcgcagcaa
    49261 cctgcggcat tgtctggtta agaatgcgtg cgccctggga accacccact accaacacac
    49321 gaaccggacc ttcacgtcca gccaaacgtt gctgcggcaa cggcagcgcc agcacatcgg
    49381 tacgcaccgg gttacccacc acttccgcat tagggaaagc gcctggaaac gcctgcatca
    49441 ctttggtagc aatcttcgcc agccatttat tggttaagcc cgcaataccg ttttgttcat
    49501 gaagtacaac cggaatgcct aacgaccacg cggccagacc acctggacct gatacatagc
    49561 cgcccatacc gagtaccacg tcaggtttgt acgctttcat aatcgcccgc gcctgacgcc
    49621 aggcgttgaa gatacgcagc ggcgcagcta tcagtgcttt tatacctttt ccacgcaaac
    49681 cagagatacg aatgaaatca atttcgatgc catgttttgg cactaagtcc gcttccatac
    49741 ggtcggcagt ccccagccag cgaacttgcc aaccctgagc cattagatag tgcgcaaccg
    49801 ccagtcccgg gaatacatgt ccaccggttc cgcctgccat caccattaat cgctttcctt
    49861 gaccactcat cgtgaacctc gtacaaacgc ctgcgctttc tccagacgcg tttcataatc
    49921 aatacgcaac agcatcatga tggctgtcga cataatcagt aagctcgaac caccgtaact
    49981 gattagcggc aatgtcagac ctttggtcgg taacatcccc gccgccgcgc ctacgttaac
    50041 cagcgcctgg aagctaaacc agatgccaat agaacaggcg agaaaaccgg aaaaacggtg
    50101 gtcaatttct aatgctttgc ggccaatcga catcgcacga aaagcgacga agaataccat
    50161 taaaagtgct agtaccacac cgacataacc taattcttcg ccgataatgg cgaaaataaa
    50221 gtcagtgtgc gcttccggca gatactccag tttttgtacc gaattaccta aaccttgccc
    50281 ccaaagttcg ccgcgaccaa acgccatcag cgattgcgtt aactgatagc cgctgccaaa
    50341 gggatcttcc cacgggttcc agaatgcggt aacacggcgg atacggtacg gttcggcgag
    50401 gatcagcaac acaaccgctg aaatgcccat accgataatg gcaatgaact gccacaattt
    50461 cgctcccgcc aggaacaaca tcgccagcgt agtcacaaac aacaccacca ccgtaccaag
    50521 gtctggctgt gccagcagta acactgccaa caccagaatc acgcccatcg gtttcaggaa
    50581 gccgcgcagg ttattacgta cttcgtcgcc tttacgcacc agatagttgg cgatatagca
    50641 aaacagcgac agttttgtca gctccgcagg ctggatacgc agcaaaccga gatcgatcca
    50701 acgcgatgcc cctttaacag cgctacccac taccagcacg atcatcagca ggatgataga
    50761 tccgagcagc atcgtggcac tgtagcgttg ccagaactcc atcggcagac gcagcgtaat
    50821 gatcgccaga ataaacgcca aaatcagata gacaccatca cgcttcgcga agaagaacgg
    50881 atcgttggtt aagcgttgcc ctatgggcat tgacgccgag gtcaccatga taaagccaat
    50941 cgccgcgagg ccgaaggtca gccacagtaa ggtgcgatcg tacatgatca ggctgtcggt
    51001 atctttttcc cgcgagccca tcacccagcc ctttagcgcc gtggagatcc agaccaggat
    51061 actgaatcct ggcaggcgcg gcattttcag gcgagggaga gataaacgca tcaacctaac
    51121 tccttcgcca gacgggcaaa ctcattgcct cgttgttcaa agttcttgaa ctgatcaagg
    51181 ctggcacagg ccggggagag cagaaccata tcgcccggct gaacacgcgg agccagcaag
    51241 cgcatagcct gttccatagt ttcggtttgt tctgccactt ccgggcgtag cgccgccagc
    51301 tgcgcgccgt cacgaccgaa acaatacaga cgtacgttat cgccattcag gtaacgcacc
    51361 agtgggctaa agtccgccga tttaccatcg ccgcccagca acaaatgcag cgtgccgtct
    51421 acgtgcaggc cattcagcgc cgcttccgta ctgccgacgt tggtcgcttt tgaatcgtta
    51481 atccagcgca caccgttatg ctccagcacc acttcaaagc gatgcggcag accggtgaag
    51541 gtggttaacg ctttcaggct gctggcgcgc ggtaaccctg cggcatctgc cagcgccagc
    51601 gccaccagcg cattggtgta gttatgctgc ccggaaagtt tcatctcttt cacattcagc
    51661 actttctcgc ctttaacccg cagccaggtt tcgccctgct gatgattcag gtgatagtca
    51721 cccatgttga cgccaaagct gacgcagcgt tcatccgcac cgcgaatcgg cattgttaag
    51781 gcatcatcag cattaaccac gcaaactttc gcgttttcgt aaatgcgcag ttttgctgca
    51841 cgatactgtt gtaaaccaaa cgggtagcga tccatatgat cttcagtcac gttcagaatg
    51901 gtcgctgcta ccgcctgtaa gctggaggtg gtttccagct ggaagctcga cagttccagc
    51961 acgtacagtt cacactcatc atccagtagc atcaacgcag gcaggccaat attgccaccc
    52021 acaccaacgt taacccccgc cgctttcgcc atttcaccca ctagcgtggt gaccgtgctt
    52081 ttgccgttag aaccggtaat cgccacaatc ggtgcttgtg cttcgcgaca aaacagctcg
    52141 atatcgccaa cgatttcgat tccggcatca gcggcagcgc ttaaggatgg atgcgccagt
    52201 gcaataccgg gactggcgac aatcagatct gccgccatca gccattcatc attcagactg
    52261 cccgtgtggc gttctacggc ttcgggtaat ttatccaggc caggcggtgt catacgcgta
    52321 tccataacgc gcggcgtcac accgcgagcg aggaaaaagt ccacgcagga aagcccggtg
    52381 aggcccaggc cgataatgac aacattttta ccctggtaat cagccatgat taacgtacct
    52441 tcagcgttgc cagaccaatc aggaccagca tcagcgaaat aatccagaaa cgcacaatga
    52501 cgcgcggttc cggccagcct ttcagttcat agtggtgatg aatcggtgcc atgcggaaaa
    52561 tacgttgtcc gcgcagttta aaggagccga cctgcaggat gacagaaagc gtttctacca
    52621 cgaacacgcc ccccataatc accagcagga attcctgacg tagcagtacg gcgataatgc
    52681 ctaacgcacc acctaacgcc agcgaaccga catcgcccat aaagacctgc gccggatagg
    52741 tgttaaacca caggaagccc agtcctgccc cgactatcgc ggtacagaca ataaccagtt
    52801 ccccggcgtg tcgcagatac ggtatgtgta aatagctggc aaagttcatg ttgccggtcg
    52861 cccacgccac cagcgcaaaa ccaccggcga caaatacggt cggcataatt gccagaccgt
    52921 caagaccatc ggtcaggttt accgcgttgc cagtacccac aatgacgaag taagccagca
    52981 gaatgtagaa cagccccagt tgcggcatca catctttaaa gaatgggacc accagctgcg
    53041 ttgcgggcgt gtctttgccg gcaaggtaca aagcgaaagc aacacccagc gcaatgaccg
    53101 acatccagaa atatttccaa cgagcgatca accctttggt gtctttacgc accactttgc
    53161 gataatcatc aacaaagcca ataacaccgt aacctaccag caccaccaac acgcaccaga
    53221 cgtacggatt ggacgggtaa gcccacagca gcacggagat cacaatcgct gtcaggatca
    53281 taatcccgcc catggtcggc gtaccgcgct tgctgaagtg tgattcagga ccgtcgttac
    53341 gcactacctg accaaaggaa agtttttgca aatgagcaat catgcgcggg cccatccaca
    53401 atgagatgaa cagcgcggtc agcaggctga cgatggcgcg aaacgtcaga taggaaaaga
    53461 cgttaaagcc ggaataatat ttgaccaaat gttcggccag ccaaactaac atgtcccatt
    53521 ctcctgtaaa gcgcgtacta cctcttccat ggcagcacta cgtgaaccct taactaaaat
    53581 cgtaattacc tgttgctcag caatcagtga tttaagacgc gtaattaacg cagttttatc
    53641 agcaaaatgt tcgccaacgc cgctggcggt gctgatagca tggctttgtt tacccatgct
    53701 taacacgcgg tcaataccag cagcttttgc cgcctcaccc acctgtacat ggcaggcttc
    53761 gctttcagcg cccagttccg ccatatcgcc caccaccagc acgcggtagc ccggcatttc
    53821 agccagtacc tgaacagctg cggtcattga accgacattg gcgttgtagg agtcgtcgag
    53881 cagcaactgg ttttctgcca gttggatggg gaacagacgg ccaggaacag ctttcagatt
    53941 tgccagcccc gctttgatag catcaagcgt tgcgcccacg gacatggaga gcgcagcggc
    54001 tgccagcgca ttcgcaatat tgtgacgccc cggcaacggc agcagaacat cgacgctacc
    54061 ggttggggtt tgtagggtaa attccatacc gtgcgaggtc acatggatat tggtggcggt
    54121 gaaatcgctg ttggcggcat tgggtgagaa acgccacact ttgcgtgagc caattacgct
    54181 ctgccagttc agccagtcgt tgttgtcggc gttcataatg gcgataccgt tttccggcag
    54241 gccgctaaag atttcacctt tcgctttcgc gacacccgca agcgagccaa aaccttccag
    54301 atgcgccgct gccaggttgt tgaccagcgc cgcttccggg cgagtcagac tcacagtcca
    54361 ggcaatctcg ccctgatggt tcgcgccaag ttcaataact gcgtaatcgt attccggcgt
    54421 taagcgcaac agcgtcatcg gtacaccgat gtcgttgttg agattgcctg ccgtataaag
    54481 cgtgttgccg cactggctta aaatcgccgc cgtcatctct ttaacggagg ttttgccgga
    54541 ggaccccgtc agagcaacca cgagcgccgg aacttgctgg cgaacccatg cagccagttc
    54601 accaaacgcc agacgcgtat ccttgacgat taactgcggc aggtcgatgt ccagcggacg
    54661 gctaaccagt agtgcgcctg cgccgccagc tttcgcctgg tcggcaaaat cgtgggcatc
    54721 aaaacgttca cctttcaggg caacaaacag gcagcccggc gtcagttttc gggtatcagt
    54781 ggttacagca tcaagggtga tatctgcacc ttgcagttca ccgttgagaa tatcggtaag
    54841 ttggctaagg gttacgctaa tcatgcaatc ccccccagca gacgcgccac cgtgacgcga
    54901 tcggagtagt ccagacgctg attgccaaca atctggtaat cctcatggcc tttgcccgcg
    54961 accagtacca catcattctc tttagcctgc ataacggcgc aagtcaccgc ttcagcacgg
    55021 ccttccatca ctttggcatg tccggcatct aacattcccg ccagaatatc gttgatgatg
    55081 gcacgcggtt cttcggtacg cgggttatcg tccgtcacca ccgccacgtc agcaaactct
    55141 tcggcaattg cgcccatcag tggacgctta cctttatcgc gatcgccacc acagccaaag
    55201 acgcaccaca gcttgcccgc acagtgcagg cgcgccgcct gtaaggcttt ttccagtgca
    55261 tccggcgtat gcgcgtaatc caccaccacc gtcggtttgc ctggcgcagt gaacacttcc
    55321 atacgtccgc aaaccggttg cagacgcgcg gcggttttca gcagatcagc cagtggatag
    55381 cccagcgcca gcagtgttgc cagcgcgagc aacaggttgc tgacgttaaa agcgcccatc
    55441 agatggcttt caatttcgcc atcgccccag cttgaactaa agcgaatggt cgcgccgctg
    55501 tcgtgatagt tcacttcggt cgctttcaac cagcgtccgt gacagttcgg attgatatga
    55561 tcttccattg ataccgcaac cgcgtccggc agttttgcca gccagcggcg gcccacttca
    55621 tcgtcggcgt taataatcgc ctgaccgcaa tgatgttcag aataaagcag ccatttcgca
    55681 gcttcgtagt gttccatatc accatgataa tcaaggtgat cgcggcttaa gttggtaaag
    55741 accgacgccg gaaatttcaa tgccgccaca cggtgctgta ccagcccgtg ggaggaaact
    55801 tccattgcgc aaaaagtcgc gccctgatcc accagccccg ccagctcatg ctgaacatca
    55861 actgccgaac cggtagtatt ttctgtcggg atcactttcc ccagcagacc gttaccaacg
    55921 gtgcccatta ccgcgctggt ttcgccaagc agttggctcc actgcgccag aagctgggtc
    55981 gtcgtggttt tgccgttggt gcccgttacg cctacgagac gtaaattgtc agagggttca
    56041 tggtaaaagc ggcccgccag tgcagataaa cgctcgttga gctggctgag atagatgacc
    56101 ggtacgccgt gcatttcacg gatttcaccg tcggtcgcct catctttcgc ctctgcaata
    56161 atggcagcca caccttgcgc tatcgcctgc gggatatatc gacgcccgtc cgcctgatga
    56221 cctactacag ctacaaagag atcgcccgcc gcagccacac ggctgtcgag tgtcatctct
    56281 cgcagtgctc gcgaaggtgc gtctggcacc cacggagcaa gaaggtcgcg caaattacga
    56341 tctgccacct gtcccctcgc cttgattaat cacaaattca tttttatcgc ccgttgtcag
    56401 cgcatccggc tcgatgttca tggtacgcaa tacgccgccc atgatggcac caaagaccgg
    56461 cgcggaaacg gcgccgccgt agtatttacc cgcctgcgga tcgttgataa caacaaccag
    56521 cgcgaagcgc ggctgactcg caggcgcaac gcctgcggta taagcaatat atttattgat
    56581 gtagcgaccg tccggcccga cctttttcgc ggtaccggtt ttaatggcga tacgatagcc
    56641 tttaatcgcc gccttcacgc cgccgccgcc tggtagcgcc acgctttcca tcatatgcac
    56701 cacggtgcga acaatggatt ccgggaagac acgttcaccg ggaaccgggg ggtcaacttt
    56761 ggtaatcgac agtgggcgat aaatgccgta gctgccgata gttgcgtaga ctcgcgctaa
    56821 ctgtaacggt gttaccatta gcccgtagcc gaaagagaag gtggccctct ctatgtcaga
    56881 ccaccgttgt ttttgaggat ataagccact gcgttctccg accaacccca aattggtcgc
    56941 ttttcccagt ccaaaacgtg agtaagtatc tactaacgct gaggacggca tcgttaacgc
    57001 cagcttggaa acaccgacgt tactcgactt ctgtaatacc ccggtcaggg ttaattcgct
    57061 gtagcgtgcc acgtctttga tttcgtggcc gttaattcga taaggaatgg tattgagtac
    57121 cgagttttcc cgcaccacgc cacgttgcaa cgcggtcatc accaccatcg gtttaaccgt
    57181 tgagcccggt tcaaacacgt cggtgatggt acggttacgc atcgcctctt tcggcgtgcc
    57241 gctcagattg ttagggttgt atgacgggct gttagccatc gccagcactt cacctgtgtt
    57301 gacatccacc agcacggcgc taccagattc agccttgtta aaggccaccg cgttgttcag
    57361 ttcgcgataa accagcgcct gcaggcgttc atcaatactc agcgccaggt tgtgcgctgc
    57421 ctggctgtca gtagaagaaa tatcttcaat tacgcgacca tagcggtctt tacgcacaat
    57481 gcgctcaccc ggctgcccgg taagccattt atcgaaactc ttctcaacgc cctcaatccc
    57541 ttgactatcg acgttagtaa agccgatgag gtgagcagtc acttcgccgg acggatagta
    57601 acggcgagac tcttcacgca gatgaatccc cggcagtttc agttttttga tgtagtccgc
    57661 catgtcagag tttacctgac gcgccagata aataaagcgc cctttcgggt tggcgttaat
    57721 gcgggcagaa agctgatcca gcggaatatt gagcgcgtta gccagcgcct tccagcggtc
    57781 accgacgctg ataccgccag cgtcatgcac ttctttcggg tcagcccaaa tcgcttttac
    57841 cggcacgctc accgctaacg ggcgaccaga acggtcagta atcatgccgc gggaggtgga
    57901 aacttgctga acgcgaagag aacgcatgtc gccctctttc accagcatat ccggggagat
    57961 aacttgtaac cacgctacgc gtccgagcag aaaagccagc gccaggagaa tacagccgca
    58021 taacaacgca aaacgccaac tgataaagtt ggcatgttct tcctgacgtt ttggtttctg
    58081 cgttttcgcc gctgctttca tgcgtcgcgt ttatccttat ttttgcacta cgatattttc
    58141 ttgtgacgga tcaacatgct gcatttgcag cttttccgtg gcgatccttt ccacccggct
    58201 atggtcgccg agcgcattct cttcaaggat caggttgcgc cattcaatgt ctaaagcatc
    58261 tcgctccagc accagttgtt cgcgctgagc ggtcagtaaa cgggtatggt gcgccgtggt
    58321 taccacggtc accgccgtca aaataatgca aatgaacagg cagagtggca gcttcccaaa
    58381 tcgcaaaaga tcgtcaccga taacaccagg caatgcatgg cgctcgtggc ttcccatcga
    58441 tcctttaact ttgcttagag cttctgtcac tctgctgatc atgcgttcgt cctctctgca
    58501 atacgcagaa ctgaactacg ggcacgaggg ttctcagcca cctcttcttc gcccggcatt
    58561 aacttgccta gtgctcgcag ctgacggcca cccagttttt tgagctgctc ttcagtcatc
    58621 ggtaaccctg ccggaacttg cggaccgcgg ctgttttcac gcataaaacg tttcacaata
    58681 cggtcttcca gcgagtggaa gctgatgatc gaaagccgcc cacccggggc cagcacgttg
    58741 agcgagcttt ttagcgcctg ctctatctcc tccagttcac tgtttaccca aatgcgcacc
    58801 gcctggaagg tacgggtcgc gggatgttta aacttatctt tcaccggcgt tgcagcagcc
    58861 acgacttccg ccagttcttt ggtgcgggtc atcggctgtt cgcggttacg ctcgacaatg
    58921 gcgcgggcaa tgcgtttggc aaaatgctct tcaccatagg ttttcaatac ccaggcgata
    58981 tcggcttctt ctgcggtttg tagccattca gcggctgact gcccacgggt tgggtccata
    59041 cgcatgtcca gcggaccatc gcgcataaag gaaaagccgc gttcagcatc atcaagttgc
    59101 ggtgaagaga cgccaagatc gaggagaatg ccgtcgatct tgccaataag atcgcgctcg
    59161 gcaacgtatt cgcccagcgc ggagaaaggt ccgtggatga tggagaagcg cggatcatca
    59221 atagtcttcg caacggcgat agcctgcggg tcgcgatcga tcgccagcaa acgcccctct
    59281 tcgccaagct gcgagaggat cagacgtgag tgaccaccgc gaccaaaagt cccatcaatg
    59341 tagatgccat caggacggat attgaggcca ttaacggctt catccagcag caccgtagta
    59401 tgtttatagt tttccatcat tttatagaga caagtcctgc agtcgctccg ataagtctcc
    59461 ggtagccaac tgctctgcgt cgatatcttc cttgacctgt tgatgccagg ttgtttcatc
    59521 ccacagctca aacttgttga actgtccaac cagcatcact tcttttgtca gcccggcatg
    59581 ttgccgcagt actggcgcga ttaacaatcg acctgcgcca tccatctgac attcgctggc
    59641 atgacctaac agtaggcgct gcacacggcg ctcaaccggg ttcatgctca acagacgcga
    59701 taatttttgc tcgataattt cccattcagg cagggggtaa agcagcaggc acgggtgatg
    59761 aatgtcaatg gtgcaaacca tttgaccggc agcgttctca agcagctgtt cccgataacg
    59821 ggtaggcact gataagcgcc ctttgctgtc gagattgact aacgttgctc cccggaacat
    59881 gccagcctca ccccttattc ccactttgcc ccacaatttc ccactgaaag gagtttacgg
    59941 agcggaggaa aagcttgtca agccagtcac aaggcatacc gaggtaaaaa acccgatgtt
    60001 tacggctaat atcagcgttg tttattttac gtccaacgtg cgaagcaaat taacgttatg
    60061 aatagctgta agaaaaaatc cctcatcgtc atcactgttt taaaatgact cactattttc
    60121 cacagcaaat ataaagtgtc agtttgcgac gcgagcggca ttttaggaca tatcttcccc
    60181 ggttaacagt ccttgttacg tctgtgtggc gcttgatgcc agcgggtttg cgcaaaagca
    60241 aagaaaagtg tttgttaaat cggcaattca tcgccgcatg taacaaaata atgcaaaaac
    60301 gcgctgtcga tatcacttaa ttattgtgaa tttcagctgg tgagttaatt aacgggcggg
    60361 tttaaaacca tttatcaata atagtcagga atagtcttat ttactttaag gatattgat
//
LOCUS       DAEBXN010000008        59925 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.008, whole genome shotgun sequence.
ACCESSION   DAEBXN010000008 DAEBXN010000000
VERSION     DAEBXN010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 59925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59925
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.008"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            533..1822
                     /gene="sdaC"
                     /locus_tag="KJL99_000494"
     CDS             533..1822
                     /gene="sdaC"
                     /locus_tag="KJL99_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HBD4435358.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            1880..3247
                     /gene="sdaB"
                     /locus_tag="KJL99_000495"
     CDS             1880..3247
                     /gene="sdaB"
                     /locus_tag="KJL99_000495"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HBD4435359.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            3358..4113
                     /gene="xni"
                     /locus_tag="KJL99_000496"
     CDS             3358..4113
                     /gene="xni"
                     /locus_tag="KJL99_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HBD4435360.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKADRPPMPEELHDEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(4166..5314)
                     /gene="fucO"
                     /locus_tag="KJL99_000497"
     CDS             complement(4166..5314)
                     /gene="fucO"
                     /locus_tag="KJL99_000497"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HBD4435361.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEGARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDISALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(5342..5989)
                     /gene="fucA"
                     /locus_tag="KJL99_000498"
     CDS             complement(5342..5989)
                     /gene="fucA"
                     /locus_tag="KJL99_000498"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HBD4435362.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            6536..7852
                     /gene="fucP"
                     /locus_tag="KJL99_000499"
     CDS             6536..7852
                     /gene="fucP"
                     /locus_tag="KJL99_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HBD4435363.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSSHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEILGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            7885..9660
                     /gene="fucI"
                     /locus_tag="KJL99_000500"
     CDS             7885..9660
                     /gene="fucI"
                     /locus_tag="KJL99_000500"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HBD4435364.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            9769..11188
                     /gene="fucK"
                     /locus_tag="KJL99_000501"
                     /pseudo
     CDS             9769..11188
                     /gene="fucK"
                     /locus_tag="KJL99_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
     gene            11190..11612
                     /gene="fucU"
                     /locus_tag="KJL99_000502"
     CDS             11190..11612
                     /gene="fucU"
                     /locus_tag="KJL99_000502"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HBD4435365.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMEPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            11670..12401
                     /gene="fucR"
                     /locus_tag="KJL99_000503"
     CDS             11670..12401
                     /gene="fucR"
                     /locus_tag="KJL99_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HBD4435366.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(12445..13545)
                     /gene="rlmM"
                     /locus_tag="KJL99_000504"
     CDS             complement(12445..13545)
                     /gene="rlmM"
                     /locus_tag="KJL99_000504"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HBD4435367.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(13538..13933)
                     /locus_tag="KJL99_000505"
     CDS             complement(13538..13933)
                     /locus_tag="KJL99_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HBD4435368.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(13952..14869)
                     /gene="gcvA"
                     /locus_tag="KJL99_000506"
     CDS             complement(13952..14869)
                     /gene="gcvA"
                     /locus_tag="KJL99_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HBD4435369.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGFNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(15220..15447)
                     /locus_tag="KJL99_000507"
     CDS             complement(15220..15447)
                     /locus_tag="KJL99_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HBD4435370.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDIGMISYKDANGNKQQINRTDVKEMVELEQ"
     gene            15639..16844
                     /gene="csdA"
                     /locus_tag="KJL99_000508"
     CDS             15639..16844
                     /gene="csdA"
                     /locus_tag="KJL99_000508"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HBD4435371.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTRAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGTVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            16844..17287
                     /gene="csdE"
                     /locus_tag="KJL99_000509"
     CDS             16844..17287
                     /gene="csdE"
                     /locus_tag="KJL99_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HBD4435372.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGNSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(17338..18144)
                     /gene="tcdA"
                     /locus_tag="KJL99_000510"
     CDS             complement(17338..18144)
                     /gene="tcdA"
                     /locus_tag="KJL99_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HBD4435373.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(18382..19479)
                     /gene="mltA"
                     /locus_tag="KJL99_000511"
     CDS             complement(18382..19479)
                     /gene="mltA"
                     /locus_tag="KJL99_000511"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HBD4435374.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFVEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            19688..19764
                     /locus_tag="KJL99_000512"
     tRNA            19688..19764
                     /locus_tag="KJL99_000512"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19722..19724,aa:Met,seq:cat)
     gene            19798..19874
                     /locus_tag="KJL99_000513"
     tRNA            19798..19874
                     /locus_tag="KJL99_000513"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19832..19834,aa:Met,seq:cat)
     gene            complement(19949..21202)
                     /gene="amiC"
                     /locus_tag="KJL99_000514"
     CDS             complement(19949..21202)
                     /gene="amiC"
                     /locus_tag="KJL99_000514"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HBD4435375.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEEEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            21434..22765
                     /gene="argA"
                     /locus_tag="KJL99_000515"
     CDS             21434..22765
                     /gene="argA"
                     /locus_tag="KJL99_000515"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HBD4435376.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     LHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(22827..24653)
                     /gene="recD"
                     /locus_tag="KJL99_000516"
     CDS             complement(22827..24653)
                     /gene="recD"
                     /locus_tag="KJL99_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HBD4435377.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASNPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHTIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(24653..28195)
                     /gene="recB"
                     /locus_tag="KJL99_000517"
     CDS             complement(24653..28195)
                     /gene="recB"
                     /locus_tag="KJL99_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HBD4435378.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLIVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEPPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLLITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDTFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDLHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(28188..31076)
                     /gene="ptrA"
                     /locus_tag="KJL99_000518"
     CDS             complement(28188..31076)
                     /gene="ptrA"
                     /locus_tag="KJL99_000518"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HBD4435379.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMINANGYTQRLPQLFQALLEGY
                     FSYTATEEQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(31252..34620)
                     /gene="recC"
                     /locus_tag="KJL99_000519"
     CDS             complement(31252..34620)
                     /gene="recC"
                     /locus_tag="KJL99_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HBD4435380.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNSLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEAPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FHTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(34633..34983)
                     /locus_tag="KJL99_000520"
     CDS             complement(34633..34983)
                     /locus_tag="KJL99_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBD4435381.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVLMVLMVLMVMIVTALSGIQR
                     TSMNSLASRNQYQKLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGG
                     REGEMTRLHCPNCQ"
     gene            complement(34968..35375)
                     /locus_tag="KJL99_000521"
     CDS             complement(34968..35375)
                     /locus_tag="KJL99_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HBD4435382.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(35372..35935)
                     /locus_tag="KJL99_000522"
     CDS             complement(35372..35935)
                     /locus_tag="KJL99_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HBD4435383.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(35926..36396)
                     /locus_tag="KJL99_000523"
                     /pseudo
     CDS             complement(35926..36396)
                     /locus_tag="KJL99_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            complement(36580..37374)
                     /gene="thyA"
                     /locus_tag="KJL99_000524"
     CDS             complement(36580..37374)
                     /gene="thyA"
                     /locus_tag="KJL99_000524"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HBD4435384.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGVKAPVAI"
     gene            complement(37381..38256)
                     /gene="lgt"
                     /locus_tag="KJL99_000525"
     CDS             complement(37381..38256)
                     /gene="lgt"
                     /locus_tag="KJL99_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBD4435385.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(38407..40653)
                     /gene="ptsP"
                     /locus_tag="KJL99_000526"
     CDS             complement(38407..40653)
                     /gene="ptsP"
                     /locus_tag="KJL99_000526"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBD4435386.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSCIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(40666..41196)
                     /gene="rppH"
                     /locus_tag="KJL99_000527"
     CDS             complement(40666..41196)
                     /gene="rppH"
                     /locus_tag="KJL99_000527"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HBD4435387.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            41881..42570
                     /gene="mutH"
                     /locus_tag="KJL99_000528"
     CDS             41881..42570
                     /gene="mutH"
                     /locus_tag="KJL99_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HBD4435388.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALDGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVARLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            42639..43352
                     /locus_tag="KJL99_000529"
     CDS             42639..43352
                     /locus_tag="KJL99_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HBD4435389.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASISWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            43490..43708
                     /gene="ygdR"
                     /locus_tag="KJL99_000530"
     CDS             43490..43708
                     /gene="ygdR"
                     /locus_tag="KJL99_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HBD4435390.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            43816..44856
                     /locus_tag="KJL99_000531"
     CDS             43816..44856
                     /locus_tag="KJL99_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HBD4435391.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(44889..46079)
                     /gene="lplT"
                     /locus_tag="KJL99_000532"
     CDS             complement(44889..46079)
                     /gene="lplT"
                     /locus_tag="KJL99_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HBD4435392.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILLAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASAIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAARPGQSWNLINMTRSFLNACTSLWCNGETR
                     FSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKLV
                     TLETVSRCMPAGILIGVVVPIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERGK
                     KSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWIW
                     QRRH"
     gene            complement(46072..48231)
                     /gene="aas"
                     /locus_tag="KJL99_000533"
     CDS             complement(46072..48231)
                     /gene="aas"
                     /locus_tag="KJL99_000533"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HBD4435393.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPDL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            48817..49848
                     /gene="galR"
                     /locus_tag="KJL99_000534"
     CDS             48817..49848
                     /gene="galR"
                     /locus_tag="KJL99_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HBD4435394.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(49855..51117)
                     /gene="lysA"
                     /locus_tag="KJL99_000535"
     CDS             complement(49855..51117)
                     /gene="lysA"
                     /locus_tag="KJL99_000535"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HBD4435395.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFEVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDTLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            51239..52174
                     /gene="lysR"
                     /locus_tag="KJL99_000536"
     CDS             51239..52174
                     /gene="lysR"
                     /locus_tag="KJL99_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HBD4435396.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPTGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGISVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(52161..52853)
                     /gene="ygeA"
                     /locus_tag="KJL99_000537"
     CDS             complement(52161..52853)
                     /gene="ygeA"
                     /locus_tag="KJL99_000537"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HBD4435397.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQMLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(52982..54400)
                     /gene="araE"
                     /locus_tag="KJL99_000538"
     CDS             complement(52982..54400)
                     /gene="araE"
                     /locus_tag="KJL99_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HBD4435398.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGAAFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(54715..55476)
                     /gene="kduD"
                     /locus_tag="KJL99_000539"
     CDS             complement(54715..55476)
                     /gene="kduD"
                     /locus_tag="KJL99_000539"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HBD4435399.1"
                     /translation="MILSAFSLQGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(55506..56342)
                     /gene="kduI"
                     /locus_tag="KJL99_000540"
     CDS             complement(55506..56342)
                     /gene="kduI"
                     /locus_tag="KJL99_000540"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HBD4435400.1"
                     /translation="MDVRQSIHGAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFNDMDHVGVKD
                     LR"
     gene            complement(56629..57810)
                     /locus_tag="KJL99_000541"
     CDS             complement(56629..57810)
                     /locus_tag="KJL99_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HBD4435401.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGEQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            58065..59294
                     /locus_tag="KJL99_000542"
                     /pseudo
     CDS             58065..59294
                     /locus_tag="KJL99_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
BASE COUNT        14798 a        16076 c        14592 g        14459 t
ORIGIN      
        1 ttgccgggtt ttctcgtttt tggtcactta ctcatcaact catttcattt gttatatgaa
       61 tgtttcttac caccctcacg cgacaaatat catcacagtt aatatgtcat acaatttatg
      121 ttgcaacaca aacgtttccc tattttcata aaccgtattt tatcgctatg aaaaagaaat
      181 tatcgccatg attaactaaa agtattgatt ttttcagttc aacctacata tattgcgcgc
      241 cccggaagaa gtcagatgtc gtttaatggg caaatattgc ccttaaattc tcttttactt
      301 ttgatttaca gagtaaagcg ttgggataat ctatcttcca agcagattat tgtatttggg
      361 atcaagatca ctgatagata cataacttgt gtgtatcttt ccgccctcaa attattacgg
      421 cggtaaatga ttaagccatc gccgatagac agatttcatt tttacggtca ggcaccttcc
      481 cgggctgaac tggctaaaag ctgaattatt tgcattcctc caggagaaat agatggaaac
      541 gactcaaacc agcacgattg cgtcgaaaga ctctcgtagt gcctggcgca agacagacac
      601 catgtggatg ctgggccttt acggcacggc aatcggcgcg ggcgtgctgt tcctgccaat
      661 caacgccggt gttggcggta tgatcccgct gatcatcatg gctatccttg cgttcccgat
      721 gacgtttttt gctcaccgcg gcctgactcg cttcgtactg tctggtaaaa acccgggcga
      781 agacatcacc gaggttgtag aagaacactt tggtattggc gcaggtaaac tgattaccct
      841 gctctacttc ttcgctatct acccgatcct gctggtttat agcgtggcaa tcaccaatac
      901 cgttgaaagc ttcatgtctc accagctggg tatgacgcca ccgccgcgtg cgattctgtc
      961 gctgatcctg atcgtgggta tgatgaccat cgttcgcttt ggtgagcaga tgatcgttaa
     1021 agcgatgagt attctggtat tcccgtttgt tggcgtactg atgctgctgg ctctgtacct
     1081 gatcccgcag tggaacggcg cagcactgga aacgctgtct ctggacactg catctgcaac
     1141 cggaaacggt ctgtggatga ccctgtggct ggcaattccg gtaatggtgt tctcgttcaa
     1201 ccactctccg atcatctctt ctttcgccgt tgcgaagcgt gaagagtacg gcgatatggc
     1261 agaacagaaa tgctcgaaga tcctggcatt cgcacacatc atgatggtgc tgaccgtaat
     1321 gttcttcgtc ttcagctgcg tactgagcct gactccggca gacctggctg cggctaaaga
     1381 gcagaacatc tcgattctgt cttacctggc taaccacttt aacgcaccgg ttatcgcgtg
     1441 gatggctccg attatcgcga ttatcgctat caccaaatcc ttcctcggcc actacctggg
     1501 cgcacgtgaa ggcttcaacg gtatggtgat taaatctctg cgtggtaaag gtaagtctat
     1561 cgaaatcaac aagctgaacc gtatcactgc gctgttcatg ctggtaacga cctggattgt
     1621 tgccaccctg aacccgagca tcctgggtat gattgaaacc ctgggcggcc caatcatcgc
     1681 gatgatcctg ttcctgatgc cgatgtacgc aattcagaaa gtaccggcaa tgcgtaagta
     1741 cagcggtcac atcagcaacg tattcgttgt cgtgatgggt ctgattgcaa tctccgcaat
     1801 cttctactct ctgttcagct aagtcctttc gcgccgcttt cgggcggcgc ttcctccgtt
     1861 ttaacgcgat gtatttccta tgattagcgt attcgatatt ttcaaaatcg gcattggccc
     1921 ttccagttct cataccgttg gaccaatgaa agcgggtaaa caatttaccg acgatctgat
     1981 tgcccgtaac ctgcttaaag acgtgacccg cgtggtggtt gacgtgtacg gctcgctctc
     2041 tctgactggt aaaggccacc acacagatat cgccattatt atgggcctgg cgggtaacct
     2101 gccggatacc gtggatatcg attccatccc cggttttatt caggatgtga atactcatgg
     2161 tcgcctgatg ctggcaaacg gtcagcatga agtggagttc ccggttgatc agtgcatgaa
     2221 cttccatgca gacaaccttt ctctgcatga aaacggtatg cgcattaccg cgctggcggg
     2281 cgataaagtc gtttacagcc agacttacta ctctattggc ggtggcttta tcgttgatga
     2341 agagcatttt ggccagcagg atagcgcacc ggttgaagtt ccttatccgt acagttcagc
     2401 agccgatctg caaaaacatt gtcaggaaac cgggctgtca ctctctggcc tgatgatgaa
     2461 aaacgagctg gcgctgcaca gtaaagaaga gctggaacag cacctggcga acgtctggga
     2521 agtcatgcgt ggcggtattg agcgcggtat ttccaccgaa ggcgtgttgc ctggcaaact
     2581 gcgcgttcca cgccgtgctg cggcactacg ccggatgctg gtcagccagg ataaaaccac
     2641 cactgacccg atggcggttg ttgactggat caacatgttt gcactggcag tgaacgaaga
     2701 gaacgctgct ggcggtcgcg tggtgactgc gccgactaac ggtgcgtgcg ggattatccc
     2761 ggctgtgctg gcgtactacg ataagtttat ccgcgaagtg aacgctaact cactggctcg
     2821 ttacctgctg gtagccagcg ccattggttc tctttataag atgaacgcgt cgatttctgg
     2881 tgccgaagtg ggttgccagg gtgaagttgg cgtggcgtgc tctatggcgg cagctggtct
     2941 ggcagaactg ttaggcgcaa gcccggcgca ggtttgcatc gcagcggaaa tcgcgatgga
     3001 gcacaacctc ggtctgactt gtgacccggt cgcggggcag gttcaggtac cgtgcattga
     3061 gcgtaacgcc attgcagcag tgaaagcggt gaacgccgcg cgtatggcac tgcgccgtac
     3121 cagcgaaccg cgcgtctgcc tcgataaagt tatcgaaacc atgtacgaaa caggtaaaga
     3181 catgaacgcc aagtaccgcg aaacctctcg cggcggcctg gcgatgaaga tcgttgcctg
     3241 cgattaatct ctcccagagg cctcgttttg cgaagcctct tcctgttttc tcaaccagcc
     3301 atagcctgtt ccggcatcga atgttaccct tatcgcctga tctttaaggg ggttatcgtg
     3361 gctgttcatt tgcttattgt cgatgcactg aatcttatcc gtcgcattca tgccgttcag
     3421 ggttcgccct gtgtcgaaac ctgccagcat gcgctcgatc agctcattat gcacagccag
     3481 ccaacccacg cggtcgccgt ttttgatgat gaaaaccgca gtagcggctg gcgtcatcag
     3541 cgtttaccag attacaaagc ggatcgaccg ccaatgccgg aagagttgca cgacgagatg
     3601 cctgcattac gcgccgcctt tgagcaacgc ggcgtcccgt gctggtcagc cagcggcaac
     3661 gaagccgatg acttagccgc cacactggcg gtcaaagtga cacaggccgg gcatcaggca
     3721 acgattgttt cgacagataa aggctactgt cagttacttt caccgacatt acgtattcgt
     3781 gattacttcc agaaacgttg gctggatgcg ccatttatcg ataaagaatt tggcgttcaa
     3841 ccgcagcagt tgcccgatta ctggggactt gcggggatca gcagttcaaa ggtaccgggt
     3901 gttgcgggaa tcggaccaaa aagcgccacg cagctgctgg tcgagtttca gagtctggaa
     3961 gggatatatg agaatctgga tgcggttgcc gaaaagtggc gcaaaaaatt agaaacccat
     4021 aaagagatgg cgtttctgtg ccgcgatatt gcccgcttac aaaccgattt gcatatcgac
     4081 ggcaatttac agcaattgcg gttggtacgg taacggcgag ccggatacgc cgcaaatcgt
     4141 atccggcatt atcacatcag cgcatttacc aggcggtatg gtaaagctct acaatatcct
     4201 caagcgttgc ttcacgcggg ttgccaccgg tacaaacatc atccagtgcc gcctgagcca
     4261 gtgccgaaat gtcttccttg cgtacaccaa catcacgcaa atgtggcgga ataccgacat
     4321 cacggttgag ggcaaacacc gcttcaacag cggcattacg cgccccttcc aggctcatac
     4381 cttccacttt cacgcccata acgcgcgcga tatcgcggta cttctcaccg gtaaagtcag
     4441 cgttatagcg catgacatgc ggtaacagga tggcgttcgc aacaccgtgt ggagtgttat
     4501 aaaacgcgcc cagtggatgc gccataccat gcaccaaccc taacccaaca ttcgagaagc
     4561 ccatacccgc aacatactgc ccgagcgcca tttcttctcc ggcatcctta tcaccagcaa
     4621 ccgatcctcg cagcgcccca gcaatgattt caatcgcttt aatgtgcagt gcatcggtta
     4681 gtgcccacgc gccacgggta atatacccct caatagcatg agtgagcgca tcaacacccg
     4741 tcgcggcttt cagcgccgga ggcataccat ccatcatgtc agcatcaata aacgccacct
     4801 gcgggatatc atgcggatca acgcaaacaa acttgcgccg tttttcttcg tcagtgatca
     4861 cgtagttaat ggtcacttct gccgcagtac ctgctgtggt ggggattgcc agaatcggta
     4921 cactgggttt attggtcggg gaaagccctt ccaggctacg cacatcggca aactccgggt
     4981 tgttgctgat aatgccaatt gctttacaag tatcctgtgg agaaccacca ccaatagcga
     5041 tcaggtaatc cgcgccgcta ttctggaata caccgagccc ttctttgacg acagtaattg
     5101 ttgggttggg cactacgccg tcgtaaatcg cccatgccag ccctgcagca tccatcttat
     5161 cggtcacttt cgccaccacg ccgcattgca ccagcgtttt atcggtgacg atcagcgcct
     5221 tctgataacc acggcgtttc acctcatcgg ttaaagcccc aacagcaccc cgaccaaacc
     5281 atgccgtttc gttcagaatc attctgttag ccatcatcct tctccttgtt gctttacgaa
     5341 attactcttc aatccgtaac ccataggttt tgaatttctc cagcactacg gcaatctctt
     5401 catcgctcag cactggcacc gggtccgtaa tcgccagggt cgtcaggtaa agttgcgcca
     5461 gcacttcaac ttcatgcgcc agccataacg ctttttccag attcacctca caagcgataa
     5521 gcccatgatg ttgtaacaaa gttgccttac gatttttgag agccagcgca acatgttcag
     5581 aaagttcgcg tgttccaaag gtcgcataag gcgcgcaagg aatagaatta ccgccagccg
     5641 ccgcaatcat gtagtgaata gcggggatcg atcggttaag aatggaaact gccgtgcaat
     5701 gaacggcatg attgtgaaca accgcgttgg catccggtct gctttgatag gctgccatat
     5761 ggaaacgcca ttcgcttgag gggagctttc cttcctcatg tttaccgttg ccatcaataa
     5821 agacaatatg cgactccgtc agtttttcat atggaatgcc tgtaggcgta atcagcatcc
     5881 catcctgata acgtacactg acgttccccg ctgtcccctg gttcagtccc aggcgggtca
     5941 tttccaggca agtgtcaata atctgacgag caagtttatt tcgttccatt agctacctct
     6001 ctctgattca aaacagggca ataatgttgt tcctttcaca ctattgaatt agccgtttaa
     6061 ttacccacca tcttcttcct gattaacaag aaagaaattc acaagcttat attttgtgac
     6121 ctagctcaac taatcacagt aaataactgc aagttctctt tttataaccc cgttaaaaat
     6181 gaccgctctt aaaaatattt atcaaaacgg tcatttttcc atttcccctc gccagaaatg
     6241 cccgttttcg gcacaaacaa ttaatacggt catctggttt gtgtttttta tgatttattt
     6301 tctgaaacgg gcatgaaatt tcgattatta aagtgatggt agtcacataa agtcaccttc
     6361 cagctaataa gtgtgaccgc cgtcatatta cagagcgttt tttatttgaa aatgaatcca
     6421 tgagttcatt tcagacaggc aaatattcac cgatatgaag cccgaactcg ctggttttgc
     6481 acttttgaaa acataaccga ttacgtgctt aagcttctga acctaagagg atgctatggg
     6541 aaacacatca atacaaacgc agagttaccg tgcggtagat aaagatgcag ggcaaagcag
     6601 aagttacatt attccattcg cgctgctgtg ctcactgttt tttctttggg cggtagccaa
     6661 taaccttaac gacattttat tacctcaatt ccagcaggct tttacgctga caaatttcca
     6721 ggctggcctg atccaatcgg ccttttactt tggttatttc attatcccaa tccctgctgg
     6781 gatattgatg aaaaaactca gttataaagc agggattatt accggattat ttttatatgc
     6841 cttgggcgct gcattattct ggcccgctgc agaaataatg aactacacct tatttttagt
     6901 tggcctattt attattgcag ccggattagg ttgtctggaa actgccgcaa acccttttgt
     6961 tacggtatta gggccggaaa gcagtagtca cttccgctta aatcttgcgc aaacatttaa
     7021 ctcgtttggc gcaattatcg cggttgtctt tgggcaaagt cttattttgt ctaacgtgcc
     7081 acatcaatcg caagacgttc tcgataaaat gtctccagag caattgagtg cgtataaaca
     7141 cagcctggta ttatcggtac agacacctta tatgatcatc gtggctatcg tgttactggt
     7201 cgccctgctg atcatgctga cgaaattccc ggcattgcag agtgataatc acagtgacgc
     7261 caaacaagga tcgttctccg catcgctttc tcgtctggcg cgtattcgtc actggcgctg
     7321 ggcggtatta gcgcaattct gctatgtcgg cgcacaaacg gcctgctgga gctatttgat
     7381 tcgctacgct gtagaagaaa ttctaggtat gactgcaggc tttgccgcta actatttaac
     7441 cggaaccatg gtgtgcttct ttattggtcg tttcaccggt acctggctca tcagtcgctt
     7501 cgcaccacac aaagtcctgg ccgcctacgc attaatcgct atggcactgt gcctgatctc
     7561 agccttcgct ggcggtcatg tgggcttaat agccctgact ttatgcagcg cctttatgtc
     7621 gattcagtac ccaacaatct tctcgctggg cattaagaat ctcggccagg acaccaaata
     7681 cggttcgtcc ttcatcgtta tgaccattat tggcggcggt attgtcactc cggtcatggg
     7741 ttttgtcagt gacgcggcgg gcaacatccc cactgctgaa ctgatccccg cactctgctt
     7801 cgcggtcatc tttatctttg cccgtttccg ttctcaaacg gcaactaact gaacatattt
     7861 tccgaataaa gtgaggaatc tgtaatgaaa aaaatcagct taccgaaaat tggtatccgc
     7921 ccggttattg acggtcgtcg catgggtgtt cgtgagtcgc ttgaagaaca aacaatgaat
     7981 atggcgaaag ctacggccgc actgctgacc gagaaactgc gccatgcctg cggagctgcc
     8041 gtcgagtgtg tcatttccga tacctgtatc gcgggtatgg ctgaagccgc tgcttgcgaa
     8101 gaaaaattca gcagccagaa tgtaggcctc accattacgg taacgccttg ctggtgctat
     8161 ggcagtgaaa ccatcgacat ggatccaacc cgcccgaaag ccatttgggg ctttaacggc
     8221 actgaacgcc ccggcgctgt ttacctggct gcggctctgg cagctcacag ccagaaaggc
     8281 atcccggcat tctccattta cggtcatgac gttcaggatg ccgatgacac atcgattcct
     8341 gccgatgttg aagaaaaact gctgcgcttt gcccgcgccg gtttggccgt cgccagcatg
     8401 aaaggtaaaa gctatctgtc gctgggcggc gtttcgatgg gtatcgccgg ttctattgtt
     8461 gatcacaact tctttgaatc ctggctggga atgaaagtcc aggcggtgga tatgaccgaa
     8521 ctgcgtcgcc gtatcgatca gaagatttac gacgaagccg aactggaaat ggcactggcc
     8581 tgggctgata aaaacttccg ctatggcgaa gatgaaaata acaaacagta tcaacgcaat
     8641 gccgagcaaa gccgcgcagt tctgcgcgaa agtttactga tggcgatgtg tatccgcgac
     8701 atgatgcaag gcaacagcaa actggccgat attggtcgcg tggaagaatc acttggctac
     8761 aacgccatcg ctgcgggctt ccaggggcaa cgtcactgga ccgatcaata tcccaatggt
     8821 gacaccgccg aagcgatcct caacagttca tttgactgga atggcgtgcg cgaacccttt
     8881 gtcgtggcga ccgaaaacga cagtcttaac ggcgtggcaa tgctaatggg tcaccagctc
     8941 accggcaccg ctcaggtatt tgccgatgtg cgtacctact ggtcaccaga agcaattgag
     9001 cgtgtaacgg ggcataaact ggacggactg gcagaacacg gcatcatcca tttgatcaac
     9061 tcaggttctg ctgcgctgga cggttcctgt aaacaacgcg acagcgaagg taacccgacg
     9121 atgaagccgc actgggaaat ctctcagcaa gaggctgacg cttgcctcgc cgctaccgaa
     9181 tggtgcccgg cgatccacga atacttccgt ggcggcggtt actcttcccg cttccttacc
     9241 gaaggcggcg tcccgttcac catgactcgt gtcaacatca tcaaaggcct gggaccggta
     9301 ctgcaaatcg cggaaggctg gagcgtggaa ttgccgaagg atgtgcatga catcctcaac
     9361 aaacgcacca actcaacctg gccaaccacc tggtttgcac cgcgcctcac tggtaaaggg
     9421 ccgtttacgg atgtgtattc ggtaatggcg aactggggtg ccaaccacgg agttctgacc
     9481 atcggccacg ttggcgcaga ctttatcacc cttgcctcca tgctgcgtat cccggtatgt
     9541 atgcacaacg ttgaagagac caaagtgtat cgtccttctg cctgggctgc gcacggcatg
     9601 gatattgaag gccaggatta ccgcgcttgc cagaactacg gtccgttgta caagcgttaa
     9661 tacattacac cctgatgtga tgcccggtcg ctccggctac cgggcctgaa caagcaagag
     9721 tggttagccg gataaacaat gttatccggc tatattgcag gagcgattat gaaacaagaa
     9781 gttatcctgg tactcgactg tggcgcgacc aatgtcaggg ccatcgcggt taatcggcag
     9841 ggaaaaattg ttgcccgcgc ctcaacgcct aatgccagcg atatcgcgat ggaaaacaac
     9901 acctggcacc agtggtcttt agactgccat tttgcaacgc tttgctgatt gctgtcggca
     9961 aatcaatagt gaactgactg aatgccacat ccgcggtatc gccgtcacca cctttagtgt
    10021 ggatggcgct ctggtagata agcaaggcaa tctgctctat ccgatcatta gctggaaatg
    10081 tccgcgaaca gcagcggtta tggacaatat tgaacggtta atctccgcac agcggttgca
    10141 ggctatttct ggcgtcggag cctttagttt caatacgtta tataagttgg tgtggttgaa
    10201 agaaaatcat ccacaactgc tggaacgcgc gcacgcctgg ctcttcattt cgtcgctgat
    10261 taaccaccgt ttaaccagcg aattcactac tgatatcacg atggccggaa ccagccagat
    10321 gctggatatc cagcaacgcg atttcagtcc gcaaatttta caagccaccg gtattccacg
    10381 ccgactcttc cctcgtctgg tggaagcggg tgaacagatt ggtacgctac agaacaacgc
    10441 cgcagcaatg ctcggcttac ccgttggcat accggtgatt tccgcaggtc acgataccca
    10501 gttcgccctt tttggcgctg gtgccgaaca aaatgaaccc gtgctctcct ccggtacatg
    10561 ggaaatttta atggttcgca gcgcctaggt tgatacttcg ctgttaagtc agtacgccgg
    10621 ttccacctgc gaactagata gccaggcatg gttgtataac ccaggtatgc aatggctggc
    10681 atccggcgtg ctggaatggg tgagaaaact gttctggacg gctgaaacac cctggcaaat
    10741 gttgattgaa gaagctcgtc tgatcgcacc tggcgcggat ggtgtgaaaa tgcagtgtga
    10801 tttattgtcg tgtcagaacg ctggctggca aggagtgacg cttaatacca cgcgggggca
    10861 tttctatcgc gcggcgctgg aagggttaac tgcgcaatta cagcgcaatc tacagatgct
    10921 ggaaaaaatc gggcacttta aagcctatga attattgtta gtcggtggag gaagtcgcaa
    10981 cacattgtgg aatcagatta aagccaatat gcttgatatt ccggtaaaag ttctcgacga
    11041 tgccgaaacg accgtcgcag gagctgcgct gttcggttgg tatggcgtag gggaatttaa
    11101 cagcccggaa gaagcccgtg cacagattca ttatcagtac cgttatttct acccgcaaac
    11161 tgaacctgaa tttatagagg aagtgtgaaa tgctgaaaac aatttcgccg ttaatttctc
    11221 ccgaactatt gaaagtgctg gcagagatgg gacatggaga tgaaattatt ttttccgatg
    11281 ctcactttcc cgcccattcg atggaaccgc aggtgatccg cgctgatggc ctgttggtga
    11341 gcgacttgct ccaggcgatt atcccgttat ttgagctgga cagttatgcc ccgccgctgg
    11401 tgatgatggc ggcggtagag ggtgacactc tcgatcctga agtagaacga cgttaccgta
    11461 atgcgctttc actacaagcc ccgtgtcctg acatcatccg catcaatcgt tttgcgtttt
    11521 atgaacgggc gcaaaaagcc tttgcgatcg ttatcacagg cgaacgagcg aagtacggga
    11581 atattctttt aaaaaaaggg gtaacaccgt aatctcatac cggtacgccc gcatgacgcg
    11641 ggcggttatc gaatgatggg gtgaaaaata tgaaagcggc acgccagcaa gcgatagtcg
    11701 acctgctgct gaaccatacc agcctgacca cggaagctct ctctgaacag ctaaaggtca
    11761 gtaaagaaac cattcgtcgc gatctcaatg aattacagac gcagggtaaa attctgcgca
    11821 atcatggacg cgctaaatat atccaccgtc aaaatcaaga cagtggcgat ccctttcaca
    11881 tcaggctgaa aagccattat gcgcataaag cagatatcgc gcgcgaggcg ctcgcgtgga
    11941 ttgaagaagg gatggtgata gccttagacg ccagttcaac ttgctggtat ctggcacgcc
    12001 agttgcctga catcaacatt caggtcttca ccaatagcca tccgatttgc catgaactcg
    12061 gtaaacgcga acgcattcaa ctgatcagtt ccggcggcac acttgagcgc aaatatggct
    12121 gttacgtcaa tccctcgctg atttcccaac ttaaatcgct ggaaatcgat ctgtttattt
    12181 tttcttgtga agggatcgat agcagcggcg cactgtggga ctccaatgcg atcaacgctg
    12241 attacaaatc gatgctatta aaacgtgccg cgcaatcgtt gttattgatt gataaaagta
    12301 aatttaatcg ttcaggggaa gcccgcatcg ggcatctgga tgaggtaacg cacattattt
    12361 ctgatgagcg ccaggttgca acttctttgg taacagcctg acggaaaggg tagcaggccg
    12421 gagacgacgc cccggccttg cctgttatcg ctcgtcgcga cgaccaccca ccgcagccca
    12481 gatgcggcgg acgtgcaccg tcacttcttc gcgatcgtga tacaactgcc gcgcctgaat
    12541 ctgagcattt atgccatgtt catcaagctg tgcctgaata tacgccagat tgtgtgatac
    12601 ttcttcgtag cgttttttca tcggcagttt gagattgaaa atggtttcac ggcaccagcc
    12661 attaaccagc cactgcgcca tcaatgccgc aactttagcc ggtttttcaa ccatatcgca
    12721 taccatccag gagatattgc tgcgtgtcgg acggaatttg aaaccgtctt cccgcagcca
    12781 cgtcacctgc ccggtatcca tcagactttg cgccatcggg ccgttgtcga cggaataaac
    12841 ccacatgttg cgcttcacca gttggtaggt ccagccgcca gggcaagcgc ctaaatccac
    12901 cgcccacatc ccgttcgcca ggcgttcatc ccactcatcc gcaggaataa acacatgaaa
    12961 tgcctcttcc agtttgagcg tggaacgact cggcgcctct gccggaaatt tcaggcgcgg
    13021 aatgcccata tagaacggcg aattattgtt gctgtatgag taaccggtat agcagcatcc
    13081 tggtgcaata aagaatacat gcacaaccgg acgcttcggc gtttcatagt tcgccagcac
    13141 cccagcatcg cgcaaggcag cgcgtagcgg aacagtgaac ttacggcaga atttcagcaa
    13201 ctctttgctt tcgttggtat cggcaacttc aacacgcagt tcaccgccct tctctactac
    13261 accctgtaac atgccgacaa tgggggtaat acgatcttct ggcggcaaat gctgcaggag
    13321 ttcccccacc acaaaccact ggcgggcaaa aattaatgaa ctgaacggca gctcacggat
    13381 taacttatcg ccatcatcag gttgataaca ttcataaatg acataacccg cattctcttt
    13441 cacgcgggca aaaccgaaaa tttcccgctg gccggcttta tcggtaattt ctgcggcgca
    13501 ctctttttca aagcccggac ggcacagcaa tacaacctta ttcatgactt acgcccttgc
    13561 gctttaaacg gatagcacca actaacatta acgcccagcc cgcaaggaag ctcacgccgc
    13621 caaccggtgt gacaaacgcc cacaaacgca gatgggacag cgccaggcaa taaaggctgc
    13681 cgctgaacag caccgtgcct aacgcgagga aaacgctact ccagtaaaac cagatactga
    13741 tgcgacgctg cattgccacc gccagaccta agatcgccag cgtatgaaac gcctggtatt
    13801 cgagaccggt ttggatccag cccatctcaa cggcccccat ggttttactt aacacatgcg
    13861 caccaaaagc gcccagtgcc acaaaaatga agccgctaat ggcggcgaaa atcagcataa
    13921 aacggctggt catggtcgta ccctacgtaa attattgttc ataacgaaag cggaattttt
    13981 cttgttcagc agcggctttc gccaggatcc attggcgaaa ggcggctatt ttacccagtt
    14041 ctgcctggct gtcatgacaa accagataaa aagcattttt actgaccaga acatcattaa
    14101 acgggcaaac aagacgtccg gcctcgattt cagattgcgc catcacgtta tttgccagcg
    14161 ccactccctg cccgtgaata gccgcttgca gcaccatggc gctatgacta aaaattggcc
    14221 cttgctgaac gttgatatga ttaaacccca actgtcgggt atatgtctgc cagtcacggc
    14281 gtgacgcatc atgtaataac gtatgtttag ccagatcttc cggggtcttc aagggttttt
    14341 cgcctgtcag cagtagcggc gaacacaccg gcaataaata ttcggcgtac agtttttcca
    14401 cccgtagccc cggccagttg ccccgaccat aaaatatcgc cacatcaaca tcatccgcca
    14461 gcttatcttc ctgacgatca accgcctgga ttcgaacgtc aattcccgga taagctgaat
    14521 taaagctgga aagtcgcgga accaaccaat gaatggcgaa actggggagt aaactgaccg
    14581 tcaacgcccc cttggcgcta cgggcctgga gtttacgcgt cgcttcggtt aattgcgaaa
    14641 atatctcttt gatatcgagg aaatagcttt gcccttcctc ggtcagcagg agtgaacgat
    14701 tacggcggcg gaacagtttt agccccaaaa aatcctcaag agacttgatt tgatgactta
    14761 ctgcggcctg ggtcacaaaa agctcttctg ctgcgcgagt aaaacttaaa tggcgtgctg
    14821 cggcatcaaa aactcgtaag gcatttagcg gtggtaatcg tttagacatg gcttttaaac
    14881 tttgatgtta aatgaattta acaattagat cacactgtgt aacctattag tttttttaat
    14941 ctgagccatt ataaattgtc cgttgagctt ctaccagcaa atacctatag tggcggcact
    15001 tcctgagccg gaacgaaaag ttttatcgga atgcgtattc tggtgaactt ttggcttacg
    15061 gttgtgatgt tgtgttgttg tgtttgcaat tggtctgcga ttcagaccat ggtagcaaag
    15121 ctaccttttt tcacttcctg tacatttacc ctgtctgtcc atagtgatta atgtagcacc
    15181 gcctaattgc ggtgcttttt tttaccttgc gatcgcgaat tactgttcca gttcgaccat
    15241 ctctttcacg tcagtacggt tgatctgctg tttgttgcca ttagcgtctt tatacgaaat
    15301 cataccgata tcgttatcag tctgtggttt gccgtcagag acgatggtac gtccgtcatt
    15361 ggtgtgcatc acatagttcg aaccggaaca ggcgctcagg gcaaaagtca gcatacaggc
    15421 agaaataatt gcggcagtct ttttcatcat cttctcctta aaacgattaa taaatcttaa
    15481 tagtattgat taacaggcta aaattaacgc ctaacactat tcagcatatg ttacttacgc
    15541 ggcttcgcca ggatatccag ataattctga tggttagcac tctccttgta tcaaagtgaa
    15601 ttttgcgtca cgatcggtgc atcaagccga ggagtaccat gaacgttttt aatcccgcgc
    15661 agtttcgcgc ccagtttccc gcactacagg atgcgggcgt ctatctcgac agcgccgcga
    15721 ccgcgcttaa acctgaagcc gtggttgaag ccactcgaca gttttacagc ctgagcgccg
    15781 gaaacgtcca tcgcagccag tttgccgaag cccaacgcct gaccgcgcgt tatgaagctg
    15841 cacgagagaa agtggcgcaa ttactgaatg caccggatga taaaactatc gtctggacgc
    15901 gcggcaccac tgaatccatc aacatggtgg cacaatgcta tgcgcgtccg cgtctgcaac
    15961 cgggcgatga gattattgtc agcgtggcag aacaccacgc taacctcgtc ccctggctga
    16021 tggtcgccca acaaactaga gccaaagtgg tgaaattgcc gcttaatgcg cagcgactgc
    16081 cggatgtcga tttgttgcca gagctgatta ctccccgtag tcggattctg gcgttgggtc
    16141 agatgtcgaa cgtgactggc ggttgcccgg atctggcgcg agcgattacc tttgctcatt
    16201 cagccgggat ggtggtgatg gttgatggtg ctcaggggac ggtgcatttc cccgcggatg
    16261 ttcagcaact ggatatcgat ttctatgctt tctctggtca caaactgtat ggcccgacgg
    16321 gtatcggcgt gctgtatggc aaatcagaac tgctggaagc gatgtcgccc tggctgggtg
    16381 gcggcaaaat gattcacgaa gtgagttttg acggtttcac cactcaatct gcgccgtgga
    16441 aactggaagc aggaacgcca aatgtcgctg gcgtcatagg attaagcgcg gcgctggaat
    16501 ggctggcaga ttacgatatc aaccaggccg aaagctggag ccgtagctta gcaacgcttg
    16561 cagaagaagc gctggcgaaa cgtccaggct ttcgttcatt ccgctgccag gattccagcc
    16621 tgctggcctt tgattttgct ggcgttcatc acagcgatat ggtgacgcta ctggcggagt
    16681 acggtattgc cttgcgggcc gggcaacatt gcgctcagcc gctactggca gaattaggcg
    16741 taaccggcac actgcgcgcc tcatttgcgc catataatac aaagagtgat gtggatgcgc
    16801 tggtgaatgc cgttgaccgc gcgctggaat tattggtgga ttaatgacaa acccgcaatt
    16861 cgccggacat ccgttcggca caaccgtaac cgcagaaacg ttacgcaata ccttcgcacc
    16921 gttgtcgcaa tgggaagata aatatcgcca gttgatcatg ctggggaaac agcttccggc
    16981 tttgccagac gagttaaaag cgcaggctaa agagattgcc ggatgcgaaa accgcgtctg
    17041 gctgggatat accgtggctg aaaacggcaa aatgcacttc tttggcaaca gcgaagggcg
    17101 cattgtgcgc ggcctgttgg cggtgttgtt gactgccgtt gaggggaaaa ccgccgccga
    17161 attacaggca cagtcgccgc tggcattgtt tgatgagctg ggattacgtg cgcagcttag
    17221 cgcctcacgc agccaggggt taaatgcgtt aagcgaggcg attatcgctg cgacgaagca
    17281 ggtttaagca cagaaccggg tcggataaga cgttcgcgtc tcatccgacc tgattgttta
    17341 accctgacgc gccgctttcg ccatcatctt cttcagcgca tgagaaaccg caacaaaccc
    17401 aaaggtggcg gtcaccatcg ttgccgcgcc aaatccagat gcacaatcca tccgcttcgg
    17461 cccttctgcc gtggctttca tcgcacacac cgtaccgtct gactgcgggt acaccagcgc
    17521 ttcagtagaa aacacgcaat ccacgccgag cttaccttta ctgtttttca ctacgccaaa
    17581 atcgcttttc aggcgctcgc gcaacttcgc cgccagcgga tcctgaatcg ttttcgccag
    17641 atcggtaacc tgaatctgcg tcgggtcaat ctgcccaccc gcgccaccgg tcgtgaccag
    17701 cgggatttta ttgcgccgac aataagcaat cagcgccgct ttgggccgta cactatcaat
    17761 ggcatcaatc acgtacgaat aaccggcact catatattgc gctacgttat ccggcgtcac
    17821 gaaatcatcc accaccgtta cacggcactc cgggttaatc tggcgaatgc gctccgccat
    17881 gacttccgct tttgccagcc cgacgttatc gcgcagagcg tgaatttgcc gattggtatt
    17941 ggtgacgcac acatcatcca tatcgataag cgtgattgcg ccaatccccg tgcgcgccag
    18001 cgcttccgcc gcccaggaac cgacaccacc gataccaacc acacaaatat gcgcgtcagc
    18061 aaacagttgc aacgcttttt caccatacag acgcgctgtg ccaccaaaac gctgacgcca
    18121 tgcatcacta attaccacag acatagcacc tcagaattaa aaagggcgag gttatccctc
    18181 accctaaacg cataaatgcc tgatgcgcta cgcttatcag gcctacataa ctcctgcaat
    18241 attttggatt tgcatgattt gtaggccgga taaggcgttc acgccgcatc cggtatgaac
    18301 aacgcgcact tagtcaataa tcaggatgtg aggttacccc tcccctgtca tatccgtaaa
    18361 aacggcatac agaatatcac atcagccgct aaagacgtta cctgcgcccg gagccgtttt
    18421 cagcacccaa acccgtccat agtggttgta ccaacctgcg cggtgtccgg cttccggccc
    18481 gatcccttga tagatatcga agtgttggcc tttgattgcg cccccgacat ccaacgccac
    18541 catcagacgc aattcgtact gaccgttaaa tttgccgtta ttatccagca gcggcacttc
    18601 tgccagcaag gtagtacctg gcggaataat tgaacgatcg gaggcaacag aagcacggcc
    18661 aaccagcggc accgcacttg cccctttcac cggtgcaaaa gattgcggtt taaagaagac
    18721 gaaagacggg ttctgttcca gcagctcgcg aacctcggct tcactgtgtg tttcgcccca
    18781 atgacgaatc gcctgcatcg acatatcttc ttttttcact tcgccacggt cgatcagcac
    18841 cttaccaatg ctgcgatagg catgaccgtt tttccctgca tagctgaaaa agttaagcgg
    18901 actgccatca ccaaagtcga tatacccact accctgcaca tccataatga agttatccat
    18961 cagagagttg ctgtaagcga ggatgtattt atcgcccaat gccccggcgt agatctccgc
    19021 acgagacggc agacgaccac gttttggcgg catacggtaa ataggatact ggaactcgcc
    19081 ctggcgggta tggcgcgcct gaattaccgg cgtgtaatag cctgtaaact gcacgttacc
    19141 atagttgtcg gcaccttcca tctgccaggc atcaatgccg aactggcgca tattgcgggt
    19201 atcaccgcct gcgcgcagcc actcttgcac cgcgttataa acattactct ggttgccata
    19261 cagacgcggt gacgaattac ggatatggtt aatttgctcg acaaaatcac cggcgttaat
    19321 cggcgcgcca acggcatctg gctggttcac cagagagaaa ggctgggtaa atttcccgtc
    19381 tttatattgc tgtccgcgat cggttggttt ggaagagcag gcggcaagca ttgccacaac
    19441 cgtgcccata agaaggtact ttacccaacg tcctttcatt gttctcttct tcaggttaaa
    19501 aaataaggcg caacgaagat aacaaaccgc cgcgtttaat gaaatgagca gcctctccct
    19561 gacgcaaatt ttgcacaaaa aataggcttt agtggtttgt ttttgttcaa aatcatgcta
    19621 aatccgtgat cggggtaaaa aaaaggttgc atgaaaacgc gagcggagta tagtgcgcat
    19681 ccacggacgc ggggtggagc agcctggtag ctcgtcgggc tcataacccg aaggtcgtcg
    19741 gttcaaatcc ggcccccgca accaatcaaa tttgatgaag taaaagcagt acggtgacgc
    19801 ggggtggagc agcctggtag ctcgtcgggc tcataacccg aaggtcgtcg gttcaaatcc
    19861 ggcccccgca accaattatt gaacacccta acgggtgttt ttttgtttct gatctcccat
    19921 aaaaaaagcg ccattcagcg cctttttatc atccccttct cgccagcgtt gccccatcgg
    19981 caaaatacgc tttaatcccc gcaagaatag actccgcaac ttcctgctgg aaagtcgccg
    20041 ttttcagttt acgctcttcc tcaacgttac tgataaacgc cgtttcgacc agaatggagg
    20101 gaatatctgg tgcctttagt acggcaaacc cggcctgttc aacttgattt ttatgcagct
    20161 tgttgatttt tccgagctta ttcagtaccg ctttaccaaa cttaaggctg tcagcaatag
    20221 tcagcgactg caccatatcg aacatggtgt ggtcgacata gcggtcaccg cttttgctca
    20281 cgccaccaat caagtccgag gcgttctggg tttgtgccag atattttgcc gcagtactgg
    20341 ttgcgccttt ggttgagagc gcaaacaccg aggaaccgct cggctgacga ctggtaaagg
    20401 cgtcggcatg gatagagaca aacaggtcag cacgctgttt ctgggctttt gctacgcgca
    20461 cttgcaacgg aatgaagatg tcttcattgc gcgtcatgta caccttcata ttgccctctt
    20521 cctcgatcag agagcgcaaa cggcgagcta tttgcaatac cacatctttt tcgcgcgttt
    20581 tgtatttccc caccgcaccg gagtcttcgc caccgtggcc agggtcaagc ataatgacaa
    20641 tcggacgatc gcgccctgct ttacccggtt gtggaccact ttgcgctggc ggcacctgct
    20701 tttcgaggtc gcctttgttg taatcctcca gcagcgccag cagcgggtcc tgcatatcct
    20761 gtgcattggc cggatagagg tccatcacca gacgctcttt aaacccggcg accggcgcaa
    20821 gggcaaacag ctgcggtttt acgttttgct ttaattcaaa aaccatgcgt acggtttgcg
    20881 ggtcaaattg cccgacgcgc gccgacttga tgaacgggtc gtctgcacga atttgtgccg
    20941 ccatcccctt gagtaccgag ttcaggttta catcttcgat atccaccacc acgcgttcag
    21001 ggttactcaa cgcgaactgc ttatatttca gctgacgatt tgattctacc gtcacgcggg
    21061 tgtagctgga cgcaggccag acgcgcaccg ccacgacctg gctaaccgca gccaggctga
    21121 cctgacttac gctcaatagc cacatagcgc ccgcgccttg cagtaaacga cgacggctga
    21181 ttgcagtgtt ggatcctgac atgcctctcc cgagcaaaag aaatctaagc tgtgtaacaa
    21241 gtaaacgact aatttgaccg gaaactttag cgaaagacgc ataatctgtc atctaataaa
    21301 cggtaaacat tctttttata ttcacggcat tactgataaa aaagtcgctc tcgcataaaa
    21361 tttacacttg caccctgcga aaaaacagaa taaaaataca ataatttcaa ataatcatgc
    21421 aaaggggtgt gccgtggtaa aggaacgtaa aaccgagctg gtcgagggat tccgccattc
    21481 ggttccctat atcaataccc accggggaaa aacgtttgtc atcatgctcg gcggtgaagc
    21541 cattgagcat gagaatttct ccagtatcgt taatgatatc gggttgttgc acagcctcgg
    21601 catccgtctg gtggtggtct atggcgcacg tccgcagatc gacgcaaatc tggctgcgca
    21661 tcaccacgaa ccgctgtatc acaagaatat acgtgtgacc gacgccaaaa cactggaact
    21721 ggtgaagcag gctgcgggaa cattgcaact ggatattact gctcgcctgt caatgagtct
    21781 caataacacg ccgctgcagg gcgcgcatat caacgtcgtt agtggcaatt ttattattgc
    21841 ccagccgctg ggcgtcgatg acggcgtgga ttactgccat agcgggcgta tccggcggat
    21901 tgatgaagac gcgctccatc gtcaactgga aagcggtgca atagtgctaa tggggccggt
    21961 cgctgtttca gtcactggcg agagctttaa tctgacctcg gaagagattg ccactcaact
    22021 agccatcaaa ctgaaagctg aaaagatgat tggtttttgc tcttcccagg gcgttactaa
    22081 tgacgacggt gatattgtct ccgaactttt ccctaacgaa gcgcaagcac gggtagaagc
    22141 ccaggaagag aaaggcgatt acaactccgg tacggtgcgc tttttgcgtg gcgcagtgaa
    22201 agcctgccgc agcggcgtgc gccgctgtca tttaatcagt tatcaggaag atggcgcgct
    22261 gttgcaagag ttgttctcac gcgacggtat cggtacgcag attgtgatgg aaagcgccga
    22321 gcagattcgt cgcgcaacaa tcaacgatat tggcggtatt ctggagttga ttcgcccgct
    22381 ggagcagcaa ggtattctgg tgcgccgttc tcgcgagcag ctggagatgg aaatcgacaa
    22441 attcaccatt attcagcgcg ataacacgac tattgcctgc gccgcgctct atccgttccc
    22501 ggaagagaag attggggaaa tggcctgtgt ggcagttcac ccggattacc gcagttcatc
    22561 acggggcgag gttctgctgg aacgcattgc cgctcaggcg aagcagagcg gcttaagcaa
    22621 attgtttgtg ctgaccacgc gcagtattca ctggttccag gaacgtggat ttaccccggt
    22681 ggatattgat ttactgcccg agagcaaaaa gcagttgtac aactaccagc gtaaatccaa
    22741 agtgttgatg gcggatttag ggtaagcgat gaaaatcacc ggatgcggca ggcgtaacac
    22801 tcgtgcgtcg catccggcaa ttacgtttat tcccgcgaac taaacaacgc cgccagacca
    22861 ctgcgccgct cagtacgagt ggcgattgcc gcacttaata tacgctcatc ggcatacagc
    22921 gacagacgac gacgcgcgcg ggtcaccgcg gtgtatacca gctctcgcgt tactaccggc
    22981 gtgcgttggc tcggtaaaat caacgccgca tggtcgaact ccgatccctg cgatttatgt
    23041 accgtcatcg cccacgtagt ttcgtgctct ggcaagcgac tcggttgcac agacttaata
    23101 ttgccgtccg gcatcgcaaa ccagacgcgc gtcccctgcc cgcgatccag cgcaataccg
    23161 atatcgccat taaacaaccc aagcgcgctg tcattacggg caatcatcac cggtcggcct
    23221 tcgtaccaac gagagtgcgg atgacgatga attttgcgct tctgttgcat aaactgttca
    23281 attcgctcat tcagtccagc cacgccaaac ggcccttccc gcagggcgca caaaagctgg
    23341 tactcattga acgcctgaat gattaaatcc ggctcggcac gcgcttgcag cagatccaga
    23401 taacgtccgt aacccgcaag agcttcctca agcatcgcaa tataatcttc gccgctctgt
    23461 aaaagtcgtt tttcgatatc agtaaaatcc tgctgaaaaa cggttttcac tgccgtttta
    23521 tcaccacggt tgatcgccgc agctaactga ccaatgccag aatcgctgcc gaaacgatag
    23581 cttttttgca gcaggcagag actgtcgcgc aaagatgccg cttctgtgcc ggttcctgcc
    23641 ggaacgtgag tccccgtcag gcggcttagc tgcctggcac gctcggcggt aaagcccgcg
    23701 ttggcataag cgcagatatc gcccagcaca gccccagcct caaccgaggc cagttgatca
    23761 cgatcgccga gaaagatcac tcgcgcatga tctggcaagg cgtcgatcag tctcgacatc
    23821 ataggcagat cgatcattga cgcttcatct accaccagca catcaagatg cagcgggtta
    23881 ccggcatgat gacgtaaacg ctggctaccc ggctgcgcgc ccagcaatcg gtgcaaagtg
    23941 ctggcatctt ccggaatgcg tttcttttgt tcatcggtca gcggtaactg tcgcaaagcc
    24001 ttgccgagag attcggttaa gcgcgcggca gctttacccg ttggtgcagc cagacggata
    24061 cggcagcgtt cgccgtcggc catttgaatt aacgctgcca gcaacttcgc cacggtggtc
    24121 gttttaccgg tgcctgggcc gccggaaatc accgagatcc gccgcgtcag cgccactgcc
    24181 gctgcaactt tttgccagtt aatttcatcg cttactggaa aaagtttgtc cagggtttgc
    24241 gccagtagag cttcatcaac ctcaatggta tgattcacct cattgaagaa gcgcgctacc
    24301 gtccgctcgt tacaccacat gcgattcaaa taaagacgat cgccacagag gatcatcggc
    24361 gtgggttcat ctcccctgct gaccgcttga gaagccagca agcattcttc ccaattttgt
    24421 agctcaccga tttcactgac acaggtcgcc aacagcggat tcgacgcctc gttattttcc
    24481 agtcgtgaaa gcggcaaaca gacgtgtccc tctccggcat catgacttaa cagtgccgcc
    24541 gcgagggtga cggcaggatg ttcatctccc gccacggtca gggcaaattg cacatccagc
    24601 gggcgtagct gtttgtgctc cacagcttcc agtaattgct tttgcaattt cattacgcct
    24661 cctccagggt cataccggca aacatctcat ccatcagggc aatcaacccg gcgttgggtc
    24721 gggttgtgta aatcccttgt tgcggatgtt ctttatcaac gccacgcagg aacagataga
    24781 taacgccgcc aaagtgaaga tcatagtcgt aatcagcaat gcgatggcgc agataacgat
    24841 gcagcgccag ggtataaagc tgatattgca gatcatagcg gtgtgcctgc atcgctgctg
    24901 ccatagcctg ttgggtgtaa gccgaactgt cttcacccag ccagttggat ttatagtcga
    24961 gcaggtaata acgcccttcg tggcggaata ccaggtcgat aaagcctttt aacatgccgc
    25021 gtacctgcat gaactccagc ggcgggcagc cagcggatag cgggtcaaac tggcgaatta
    25081 gtgcatcaag ctggctggcg atgagcggtt cgctaatcgg caggtaaaac tccatctcca
    25141 cctgtttatc gcggtcggaa agctgactca ggctaacacc cgtttcattg agaggtgcct
    25201 ggaggacagc cgtgatccac tcggtcaaca ccggttccca ctgcgattca aagccgccga
    25261 gttccagttt ttcctgtacc cagttcgggt caaccggctg ggtaaaatcg aggtcttcaa
    25321 acaaactgtg caagaatgtc cccggtgacg caccgcgcgg gaactgatgc ggtgttaacg
    25381 tcggttcttc aacgacgctg accacgcctg cggcatcaac atccagccgt ggcatcagat
    25441 cctgagcgat accgtgacca cgctgctgca agccggagta gctggtgaca cgccagttat
    25501 cgccgggcaa tcgttgtagt gtcctcgcat tcagttctgc agttaaagca tcattaacct
    25561 gccagggctg gttatcacca gtttgtgccg tttgccaggc aatatcatca tcgcataacg
    25621 cttcaataca ggtgcgaagc cctgccgcat cttgcggttc ccctttttgc agcaaacgcc
    25681 cgagcgcact ttggtggacg tcggtgtcac cttttttatc gccacgacgg cgcaccagcg
    25741 gtgcaacgcc gagactgcaa tgccaaaccg aacgcgtcag cgccacgtaa agcaaacgca
    25801 gatcttccgc cagacgttcg acctccgcga ggtcgacgct ttctggcgca gcattaagat
    25861 ccaaaactgc ctcaaacgag tggcgatcgt gataaaacgc ctgatcctgg acgcggaaat
    25921 tggtaataaa tggcaaccag accagcggat attccagccc tttcgattta tggatcgtga
    25981 caatctgcac cagatgttta tcactttcga ggcgcatttg ttggctggag gcattactgt
    26041 ctggctcgag gatatgttgc gataaccagc gtaccagcgc atgttcactt tccagctgcg
    26101 ttccggcttc ttgaagcagt tcgctgatat gcaagatatc ggtaagacgc cgctcaccgc
    26161 ctgccgttgc cagcaagttt tctgcaatgt tacgcgccga catcagcgcc cgcagcatcg
    26221 gcataacgcc acgtttgcgc cagatttgcc gataaccatc gaactcttcg actaccgcat
    26281 cccacgcatg ttcgtcatta ttcagcgttt caatatccag cgcattcagc cccatcattg
    26341 acgttgccag cgcactgcgc agggtgttct cacgttcggg cgtcatcacc gcctgcaaca
    26401 accaaagcat ttcctgcgct tccagagttt caaaaacact gtcgcggttc gaaaggtaaa
    26461 cggaagggat ttccagcaac gttaaggcat cgcgcacctg ggcggcctcc tggcggctgc
    26521 gcaccagcac gctgatgtcc gaagcacgca ccggacgcgc gtcgtcgccg ttcatcagca
    26581 acgcttcgcc ccgctgtccg gcttgtagcc agtcgcggat ttgcgcagca catacctgcg
    26641 ccatggtact ttgatagtcg ccaacgccgc agctttcgcc ttccatcagc cacattttca
    26701 tcgcaggctg cgtttcacct ttaaatacaa aacgtaacgc ctgatttttc ccggctgatt
    26761 tcactggaat aaacggtatt tcgcgaaaca tgaacgtgtc atcagtctgg ctgaaaagct
    26821 tgttcacact gttcaccatt ccaggtgcgg agcgccagtt ggtgtctaaa gtgtagtggg
    26881 catgaacttc actacgcgcc ttcatataag tgaagatatc cgcaccccgg aatgcatata
    26941 tggcctgctt cgggtcgcca atcagcaaca atgcggtttc cggctgatgg tgccagatac
    27001 ggcgaaaaat tcggtactgc tgggggtcgg tatcctgaaa ttcatcgatc attgccaccg
    27061 ggaatcgcgt acggatcgcc gctgccaaca cctcgccgct ttcgctacgc agcgcggaat
    27121 cgagccgact taacatgtca tcaaaaccca attcgccacg gcggcgtttt tcacgcgcta
    27181 ctgtttcgcg gatctcagcc aatgcgcggg tgatcaacag atcgcggatc gacaatggtt
    27241 ctgcaagcag ttgatcgatc gcctcaaaca gtggatgtcg cggggtttcc cccccggcct
    27301 tcgtgcgatc ttctaagaaa cgctgggaga atttttccag cgactccggc aactgataac
    27361 tgtttgtctc ttcttctgcc caggcgctga tcttttcgat ccatttagcc tgattgctac
    27421 ggttaaactt gcgtcgatca ataccagaag attcgatcag cgcatccagt tcacccactg
    27481 cgtcgcgcca ctgctgtttt accgtatcaa tacgcgccac aatttgcgcg tggcgggaag
    27541 ccagcgtttc atcatcgggc ggcggtgctt tgataaccgg cggttcgcct tgcagataac
    27601 gattaatatc gcgcagcaac gcctgcggcc ctttccaggt ttcaaagacg acctgggcta
    27661 tttcacgcgg cagcgggtag cagtggcgac gccagaaatc ggcgcaggcc tggtagcgta
    27721 gcagagactc atcttcaatc agctgctgct caaacagcat gccggattca aaggcattca
    27781 ggttgagcat gcgctggcaa aagccgtgaa tagtaaagac tgccgcttca tccatctgcc
    27841 gttcggctaa caacaaccac tgcgcggctt gcgctttatc gtcgatctct tccagcaggc
    27901 gttcgtacag tggattgtcg gtggtttcac gcagacaggc gatgcgcaac tcgtggatat
    27961 tgctacggat acgaccgcgc aattctgccg tggcagcctc ggtaaaggtg accaccagca
    28021 gttcttcaac gatcagcggg cggggaaagg cggcggaacc gcctagtcca agtaacaggc
    28081 gcaaatagag cgccgcaatc gtaaaggttt tgcctgtgcc ggcagaggct tcaatcaggc
    28141 gctcgccctg taagggcaag cgcaaaggat ctagtgtctc ggcgacatca ctcattcttt
    28201 tcactcatca ggggcattgt ttgctgcaac gcgctgacgt tctcccacac tttccagcct
    28261 tcaggatgca catattcggc tttcccgttc tggctgccgg aaatctgcga cagaatagcc
    28321 atgccttgcg gctcgaccac cgcctgatgg aagaaatcag caagtttttg cggcgtcagc
    28381 agttttatct gggccacgat tttatcacgc gaatcgaagc gcatattgcc gcgatcgaaa
    28441 tctttactta acttcgatgc ttcttcgccg agcgtttgcg gtgcctgcag catctgggta
    28501 attaccgcct gctggatttg cgcaaactca tctggcttca tcgctcgcaa ttttgcctct
    28561 gcggttggga aaaacgcctt gtaacgctcc cacaagaatg aaggctgttt atcattgctt
    28621 tgcaaaagga agcccatgcc ccactgacgc cccacgctca ttggaaacgc aaacacggca
    28681 tagcccaatt gttcttcggt acgcaactga ttgtagaacc acggctgtac gatctgcccc
    28741 aacagagagc tataggctga gctggtgtat tcatcgtagc cagtcggtac aaataccgct
    28801 gccagtgcgg agtcggtgct gttaccggct ttttcaaaga tgacggattg ttttttatcg
    28861 accactacat ctttgtttcg acaccactct gaaccatcag cgcccaactg tttttgcaca
    28921 tcgcgtgcca gcgttgttgc ctgggcctcg gtcatgttgc cgataaccat aaactctggt
    28981 cgagcccctg attttaaggc gtcgcgatag gccagcacct ctttcaacgt aatggagggc
    29041 aaaattttac gccgttcatc tcgcgagaag tacggcactt gcgagagcat ctgcgcgggc
    29101 ataatcgcct gctcaaacgc tttacccttt tctgcggaat ccatcatctg gttataccag
    29161 gacttcgcct gctcaagctg ctcttccgta gcggtatagc taaagtaccc ctcgagcaat
    29221 gcctggaaca gctgcggcag acgctgggtg taaccattag cattaatcat aaggccgttg
    29281 ttagcgttgg tggaaaaact tatgccacca accgacgcct ggttgcttaa ctgatcaagc
    29341 gccagccctg cgagataatc attgagcgca aacatcacct gattgcgggc gctgtccatg
    29401 gctttcggat tacgcaaaat caggctgaca tcagctttgg gctcgctggc aaaataacgg
    29461 cttggcgcat acaccacgcg cagattcgac tcatcaacaa tcagctctgg atggtcgtat
    29521 ttcttctctg acttaatcag cgagaaatca tcaggaatat aagggttaag ctctggcaaa
    29581 gagagcgcaa tgtcggcggc ttttttctgc cagtcggcga aagtttgtgc gctgatttta
    29641 tcgacctgat acggcgcatc gacaaagtaa gccgttttgt tgtgcggctc tttcgggctg
    29701 atataccaga tacgcgcatt ctgcggcgtc atcatcgcca gacgttcctt tactgcttta
    29761 gcatcgtacc gatcggcaat attgactgca tccagcgtat gctcaacagg aacgcgaatc
    29821 atggtatctg ccagccattc gacgtaatcc atatcacggg tgatcgacgg ataacggaag
    29881 tcgatatcca gcacattcgc cagttcatcg aagtattgtt tatcaatgcc tttttcacgt
    29941 aacagattga gatagctaaa aattgccgcc acaacctgat cgcgattagc caggccttta
    30001 tcagttaaag acgcagagat cgctaatacg ccgctgttgc cgttgacgat aggatcggag
    30061 ttggcgctaa tgccctcaac taatccctgc ttttgcagcc agtcagaaag tgtacctggg
    30121 ctgcgattgc caatcagata ggtaatcaat tcatcggttt tactacggaa cttcgctgag
    30181 ttgttatcga tgcgaaactc aacgcgcaac actttacgcg gcagcgcagg gacgtaatga
    30241 atgataatgc ccttttgcgc gtcggtgact accggcacgg tgatttccgg ttttttgctc
    30301 tctttgttcg gcacgcgacc aaaggtgtcc gccgccattt ttgccaactc cggcagcggt
    30361 ttattactgt aaataaccgc cttcatcaaa ttggcggaat agtacttctc gtggaaatct
    30421 ttcagcgcct gctgcaccgg attaccaggt ttgtcgctta aagtttcgag gttaccacca
    30481 gaaaactttg aaccggggtg tgccgggtta atggtttctg cgctgacctg tgccatgcgc
    30541 atcccgtcac gcgtacgcgc catggttaat tcagcgttca ctgcattacg ctcacgttcg
    30601 gcgtatttct tgtcgagcaa gggttctgca atagcatcgg ccaggcggtc taccgcaccg
    30661 ggcaaggcgt cgttctcaac ttccagatag aaagccgtgc gatacggcgc agtgctggca
    30721 ttgtgactac cgccgtgcat tttgagatat tcggccagac tgtcagcctg cgggtacttt
    30781 ttcgacccca tcagactcat atgttcaagg taatgtgcca gcccctggta cgcctcggga
    30841 tcttccagcg acccaacggg cactaccagc gccgagagcg atttaactgc ctgcggatca
    30901 gaaaccagca agaccaccat accgttatcc agacgtatag cctgatactg gcggttatct
    30961 ttatcacttt tacggatggt ttcctgaatc ggctgccatc ccgtttctgc ctgacttaat
    31021 ggtgcccaga gggcaacaaa caacaataat gctttgaacc aggtgctgcg gggcattcac
    31081 ggacctcata atcaacttta ttttctggac agattcaaca ggttaacgca aatcatctgc
    31141 cagaatttaa tcttgtgctg cacgagtcag cctatgttta tataaccatc agtccgtgac
    31201 tggtgcgcat cataaagtaa gcagatagat tgcgcaattt ttatacaaca ctcatgactg
    31261 attaaagcga aacagcggta acaggaaacg ttgcgactgt tcaacgatgg cctccattgt
    31321 ctctggtgtt aattgccgcc agagccgttg ataccagata tcatcacctt cgccacgcac
    31381 catcatgttg ccttcgtaag cctgaaggaa tttcgtacgg gctttttgca acgtggaatc
    31441 gtcatccagc atggcatcgt tttgcgcgtc ataacaggtt tttagccacg cgccgccact
    31501 ttcaggtaac accagcaatg gcgcggacat tccttcacga tacccctcaa tcagttgtga
    31561 gaggtaatgc aaagcctgtt cggctgcaag cggcggaaaa cgccactcac cgtctttgcg
    31621 tagaaaaagg cgactttcgc cattaccacc gctggcacag tagacaaggt gttccagcca
    31681 aagttgcatt ccctgcgcca cacttaataa agagggacgc cagcgcaaca ggccatccgg
    31741 ctgcacctgc ggcaaccagc cagttatctg cacaccgttg caggcgagat caatttccat
    31801 actctgcccc ggctggcgac aggcaatgac tctgtcggca agctgctgca tctcctggca
    31861 ctgtgtttcc cagaaaattt caccaaaagc gccatacggt aaatcccctg ccgcccggaa
    31921 gcggcggaac aagcgttcgg catcatcctg ctcaaccagt gcattcaata actgctgatt
    31981 gatttgataa cggctaagtc cttccagaat aaatggctcg gtgtctggga tttcgctgtc
    32041 ttcagtatgg aagttcacct gcaaacgcat ctggaagaac gcccgcaccg gatgtgccca
    32101 gaatcgttgt agcgtttcca gcggcacggt ttccggtaag gtaaacggca gcggctgaac
    32161 aaattcagaa tgtgctttac cagcctggct ggccgcaggt agccattcac gagcatagct
    32221 ttgtcgttcg cctggctggt agttttgcgg atcaaacggc atccgggtat ggaggcaagt
    32281 aagatgcgct tttacccttg cctcgctttc atcacagttg agcgcttcat cgcccggtag
    32341 ataatgactt tgcccgatgt agtcgatcag ttcctgcacc agtaccgacg ggaaacgttc
    32401 actgttatcc tgaatggaac gcccgatata gctgatatag agtttttgct gcgcggaaat
    32461 taacgcttcc aggaacaggt agcggtcgtc atcgcgacgg ctacggtcgc cacgcttcgg
    32521 tttctggctc atcaggtcaa agcccaatgg cgcaagctga cgtggataaa cgccgtcgtt
    32581 cattcccagc aggcaaacca ctttgaacgg aattgaacgc attggcatca gagtacaaat
    32641 gttaaccggt ccggcgagaa aacgctggct gatacgttct tgatccagac gctgtgccag
    32701 ttcatcacgc aatagtgaca gcggcaccgc gtcgccatac tgcgcaccta aaccttcggc
    32761 gataatcgcc tgccattgtt gttcgatcag cgtcatcgcc gcttcggttt ccgcatccgg
    32821 caggaagaag gcgttgagca tatcgcgaca aaccggcaac cactcttcta gcggacgctc
    32881 ctgtgccagc ccgcgacgcc agatgtttag ctgcattagc agtgaagcca gatgccccac
    32941 cagttctgca attaagccgc tcgattcatc ataaggtaga accgattgcc actcgccctg
    33001 cgcgctctcc atcgcgtagc ccagcaacat acgcgtcagg ccaaatcgcc aggtgtgttg
    33061 tccggtggcg gggagttcca gctcgcgaac gttgtcgtca tctatccccc aacgaatgcc
    33121 ggattcgttg acccactggc gtaaataacg cagcccttct tcggtgatgt caaaccgcgc
    33181 cgccagcacc ggcacatcca gcagcgccag cacatcctct gacacaaagc gactgtcagg
    33241 cagtgataac aggctgataa acgcttccag taccggatgt gactgccgcg cacgacggtc
    33301 ggaaatggcg taaggcaggt aacgatccgc aggtgcgcta ccaaacactg cctgaataaa
    33361 cggactgtag ctgtcgatat cagccaccat cacgatgatg tcgcgcggag taagtgtcgg
    33421 ggcttcctcc agcatcgcca gcaagcgatc gtgtaaaact tcaacttcac gctgcgggct
    33481 atggcaaacg tggaatgtga tactgctatc cagtggatca agcgggcgtt tgttatcgct
    33541 acgggaaaac tcttcgatgt tcacacccgc aacggcgcgg ttttccagtt ccagaatgtc
    33601 agactgaatg ttatgcagca ggttatctgg cgtcacatcg acaaaagcgt ccagctcctg
    33661 gctgctctcc aggtcagaaa ggagataaat gtagtcgcgc ccaagcttcc cccatgaggc
    33721 cagcagcgag ttgccgacat cctgttcacc atcgctgtta aagagctgtc cggcattttc
    33781 gctatcgcga aatagcggca attcgcgatc ttcaaaactg tgtcgacgct gacgagtcag
    33841 cagtttcgcc agataagcag gatctttaat atcgccccag taataacggc aggggttggt
    33901 aaacaggaga tggatttcaa tatgtttacc cagcgcctgg agcgcctgga gataaacagg
    33961 cggtaacgcg gaaataccgc atataaagac gcgcgaaggt aaccccggcg ggcaggtcgt
    34021 cgcggactcc agcgtttcga taaagcgctg atagagattc gcgcggtgcc agcgcggttg
    34081 tccgagttca tgggtatatt ccaccagcgc cttccacaac ggggcttgcc aggcctgtgc
    34141 ttctcccagc ccttcaacca aatgtcctgt ttcccactgt gccagccagt ccggacgata
    34201 gaccagatac tggtcaaaca ggtccgccgc ttttgaggaa agctggaaca gttttcgctt
    34261 gtcgctatcg tcagtcagat aatgccgcaa cagggtaaag tcttcgcgct ccagcaattg
    34321 cggcagcaga gtcatcagtt tccagctcat gctctgtttg ttaaaggcgc tctctttggg
    34381 gatttccggt aacacccgga cgaacatatc ccagataaag ctcgctggca gcggaaaatc
    34441 aatgtttgcc gcaataccaa acttttgcga cagggtcatt tgcagccact gcgccatacc
    34501 ggtactttgc accagaatca tctctggttc gaaaggatcg tccagccgtt cgcgttcgac
    34561 aataaactcc atcaacgctt ccagcacgtc cagacgattg gaatggtaga cccttaacat
    34621 agcggctcct gactactgac aattcgggca atgcaggcgg gtcatctcgc cttctctgcc
    34681 cccgggtgaa actagcgtaa cgctgatgct gacacatccc gcctgcgatg tctgcattcg
    34741 gttgacctgc cagttggcag gtggcgaaat cgcgcgcagt tgcgtttgct gccaaccatg
    34801 ccgccagagc ttttggtact ggtttctgct ggcgagactg ttcattgatg ttcgctggat
    34861 acccgataac gcagtgacaa tcatcaccat cagcaccatc agcaccatca gcaccatcag
    34921 caccatcgca accattacct ccggcaggct aaagccttgt tgattcttca gggaagctga
    34981 cataatgccc cctctttcag cggacaaaaa tcgctccagc cgcgtggcga aaaaacaatt
    35041 tttccatcaa tgacttcgcc tgttcgccac aacgaaacgc cttcataacc ggcaatcaat
    35101 aaggcttcat tatctgccag taaacgcaaa caaacctggg catcggttcc agcgtactgc
    35161 gagcactgaa ctgctggctg cgtctgccag gagtgcattt ttccccacgc cagcgccgac
    35221 tgaacgatgg cctggcggcg caatgactga ctttccatgc tcacgcgaga ggcaaaactg
    35281 cgatcctgct gactcattcc ttgcaatagc aagctaccca aaaccagcag catcaggacc
    35341 agagccagtg acgaaacacc tttttcgcgg ttcacaggtt gaatcctgtc acgctatagc
    35401 tggcatccac cacggtttgc ggttcagact tactggcagc acgcatatta accgtcaaca
    35461 ccggcgagaa gccgctgaca tcctgacgta cgacctgaaa agtgtcgata atgatggcat
    35521 ccggattagt gactttatcc cagcccttac cttcacagga tgtcgcaccg cgtagcgttt
    35581 ccagcacatg ctccttcaga cgaaatccaa tctggtcgga ctcttttacc ggttcgcgat
    35641 cccagatacc gttactgttc gcatcccact gcacaatgat acagtcaccc tgtccgacaa
    35701 tttccagccc ttcgccggta cagctgccat gacaataacc cgccctctgg agatgcttcg
    35761 cgacggtaaa tacccgcagc cagatttcat cttccagtgc cagcttacga gtgctcgtta
    35821 aactttcacg ctgtaacgca ggcagaaagc gtgcagcccc cagcaacaat acgctactga
    35881 tcgccatagc aatcaacact tccagcagag aaaaaccttg ctcttttaca ggcatccttc
    35941 tgtttctcct tgctgataaa gccggagtct tccccacggc gaaaccacca gccaccactc
    36001 gcccgttgag tttttgaagc gaatatgccc ggcccatgcg gtattgcgca ggccaaagaa
    36061 agcaagcgaa ggtgtcaggt cgctcatttc gacttcgggc cagcgtggca caaagaccaa
    36121 tggtgaactg ccatgacagg tattggcccc agcagcggaa ctcacaaggc accataacgt
    36181 cccctccctg ataacgctga tactgtggtc gcggttatgc cagttggcat cttcacgtaa
    36241 atagagcaaa tagtcccgcg cctagctggc ggtttgccaa agccgttgcg actgctgcca
    36301 gtattgccag ccatagagtc cacttgcgct tagcatgacc aaaatcagca tcgcgaccag
    36361 cgtttcaatc agcgtataac cacgttgtgt tttcatgccg gcagtatgga gcgaggagaa
    36421 aaaaagacga gggccagttt ctatttcttc ggcgcatctt ccggactatt tacgccgttg
    36481 caggacgttg caaaatttcg ggaaggcgtc tcgaagaatt taacggaggg taaaaaaacc
    36541 gacgcacact ggcgtcggct ctggcaggat gtttcgtaat tagatagcca ccggcgcttt
    36601 aacgcccgga tgtggatcgt agccttcaat ctcaaagtct tcgaaacggt agtcgaagat
    36661 ggattcgggt ttacgtttga taatcaactt cggcagcgga cgcggttcgc ggcttaattg
    36721 cagatgagtt tgatccatat ggttgctgta caggtgcgta tcgccaccgg tccagacaaa
    36781 atcacccact tccagatcgc actgctgcgc catcatatgc accagtaacg cgtagctggc
    36841 aatgttgaac ggcaggccga ggaagacgtc acaggagcgc tgataaagct ggcaagagag
    36901 tttgccgtct gccacataga actggaagaa tgcatggcac ggtgccagcg ccattttatc
    36961 cagttcgcct acgttccacg ctgaaacaat aatgcggcgc gaatccgggt cgtttttcag
    37021 ctggttcagt accgtagtaa tctggtcaat atgacgacca tctggcgttg gccaggcgcg
    37081 ccactgttta ccatacactg gcccgaggtc gccattttca tcggcccatt cgtcccagat
    37141 ggtgacattg ttttcgtgta gataagcaat gttagtatcg ccctgcagga accacagcag
    37201 ttcgtggatg atggaacgca ggtggcaacg tttggttgtc accagcggga atccatcctg
    37261 caggttaaaa cgcatctgat gaccaaaaat ggaaagcgtt ccggttccgg tacggtcgtt
    37321 tttctgtgtg ccttcgtcga gcactttttg catcagttct aaatactgtt tcatggttcc
    37381 tcaggaaacg tgttgctgtg ggctgcgacg atatgcccag accatcatga tcacacccgc
    37441 gacaatcatc gggatggaaa gaatttgccc catgctgatg tactgcaccc aggcaccggt
    37501 aaactgcgcg tcgggctggc ggaaaaactc aacaatgatg cgaaacgcgc cgtagccaat
    37561 caggaacaag cctgagacag ctcccattgg gcgcggttta cgaatataca ggttgaggat
    37621 aataaacagc accacacctt ccagcagcag ttcgtaaagc tgtgatgggt ggcgcggcag
    37681 cacaccgtaa gtgtcgaaaa tggattgcca ctgcgggttg gtttgcagca gcaaaatatc
    37741 ttctgtacgg gagcctggga acagcatggc aaacgggaag ttcgggtcaa cgcggcccca
    37801 caattcaccg ttaataaagt tgcccagacg cccggcacca agaccaaacg gaatgagtgg
    37861 tgcgataaaa tcagagacct ggaagaagga acgtttagta cggcgggcga agataatcat
    37921 caccacgata acgccaatca ggccgccgtg gaaagacatg ccgccgtccc agacacggaa
    37981 cagatacagc ggatcggcca taaactgcgg gaaattgtag aacagaacat aaccaatacg
    38041 tcccccgagg aagacgccga ggaagcccgc atagagtaag ttttcaactt catttttggt
    38101 ccagccactg cccggacgat tcgcccgacg tgttgccagc cacattgcaa aaatgaaccc
    38161 caccagatac atcaggccgt accagtgaag cgccacgggt cctattgaga aaatgaccgg
    38221 atcaaactcc ggaaaatgca gatagctact ggtcatctgt caccacaagt tcttgttatt
    38281 tcgctgaaag agaacagcga ttgaaatgcg cgccgcaggt ttcaggcgct ccaaaggtgc
    38341 gaataatagc acaaggggac ctggctggtt gccggatacc gttaaaagat atgtatatga
    38401 tccgcgctat aaccctccgc gaatcagccc gcccatgccg cgacgctcca taaaggctgc
    38461 aacctgatga cgaacttcgg tcgccagttg cgcttccaga ctacgctgcg caagattttc
    38521 tgcttcggca aaatcaatgc gccgcagcag gtattttacc cgcgctacag aacgtccgtt
    38581 catagacaga tggcgatacc caagcccaat gaggattgcc acgcacatgg gatcgcccgc
    38641 catttcaccg cacaaacgaa gatcgatacc atgtatttcc gcttcccggg cgatcatcgc
    38701 cagagctcgt aacattgcag gatgaagact gtcgtaaatg ttcgccaccc gggtattgtt
    38761 gcgatcaacg gccagaatgt attgagtcag atcgttggtg ccaacagaga tgaaatcgac
    38821 cctctttgcc agatgcggca gcataaatac cattgacggc acttccagca tgatgccgat
    38881 acgtggtttg ggaatttcgt aaccgatcat ctcctcgact tcacgtccgg cacgttcaat
    38941 caggcggcgt gcttcgtcaa cttcatcgag gctggtgacc atcggcaaca gaatattcag
    39001 gttgcccgta gcggcattag cacgcagcat cgcccgcacc tggatcaaga agatctccgg
    39061 ctgatcgagc gtaatgcgaa tcccacgcca acccaggcat ggattctctt cgctgatcgg
    39121 catgtaaggc agctgcttat ctgctccgac atccagcgta cgcaaggtga cgggtttatc
    39181 attgaacatt tgcagcatcc cctgatactg cgccacctgt tcttcttccg acgggaaacc
    39241 actttgcagc atgaatggga tttcagtgcg ataaagaccg atgccatcaa tacagctgcc
    39301 cagtttttct tcatgttccg ggcttaaacc agcattgagc atgactttta tacgctcacc
    39361 gctttttaac tgcgcgggta aattgacgtc atcttccgcc agacggctaa gctcaatctc
    39421 ttcactaatt agccgctgat attcttgcag cagtaccggc tccggatcga ccagcaattc
    39481 accgcgatag ccatcaacga tcagcgtcct acgatgcagc accgaaggct gaatatccgc
    39541 gcccatcacg gtagggatcc ccagcgcacg taccatgatc gcagcatggg agttggctgc
    39601 gccatctcgc acgacaacac cgactaagcg atcctggggc agctcagcaa gagttgtcgc
    39661 tgacagttca tctgccacca gaatgaaacg ttccggccag gcgttcggcc cttgattagc
    39721 gtcatcaaga tgaaacagca atcgctgacc cagcgcacgt aaatcaccag cccgctcttt
    39781 gagatagtta tcgcttagcg cggcaaactg ttcggcaaat ttttcaatga ccgtttttac
    39841 cgcccactct gccaccgagc ctttatcaac ctcggcaaac aattcgcgac gcagccgggt
    39901 gtccgaaagc aggtgcgagt aaagatcgaa aatagccgcc gtttcttttt gtgctccggc
    39961 ggcaaagcgt ttgctgtagc ggcgaaactc gtttgccgct tcttccagcg ccccggtcag
    40021 tcgttcgcgt tccagagccg gatccagcgt tgatgcctga tacacctgtt ccattaaagg
    40081 taacgtggca tcctgccagc cttcggcaat cgccacacca ggtgctgccg gtaatgcgcg
    40141 gattcgcgtc tggcgatatt gcccaaacag ggcagtcaac tgcgactgag aaagaatagc
    40201 tgccatctgg gtggcaagcg tcaccaggaa ggattcttca ctttcgtcat actggcgcaa
    40261 ctctcgttgc tgtaccacca gtacaccaag caactggcga cgttgaataa ttggtacgcc
    40321 taaaaacgcg cggaaacgtt cttcttttac ggaggggatg tatttgaagc tggggtgctt
    40381 ttgcgcatct gcaaggttta tcggttccgc cagcctgcca accaggccga cgatcccttc
    40441 atcaaacgcg agcgttacag tgcgaccgcg tggttttttc agcccccggg tcgccatcag
    40501 gtagtaacaa cgtcgatcat gatcggccag gtagaccgaa cagacctcgg tatccatcgc
    40561 aagacagatg tcggtaacca gaatatttaa cgcctcattc aggcgtggtg cgctggctac
    40621 cttttcgact atttcgcgca ggcgagtgag cataattggc gtgacttaac ctcttttacg
    40681 tcgataagca gatgcgtttt gtggttttgg cgtattttcc tgcagtgaca tcaccacact
    40741 cgcgaactct ttcattaccc tacggtagac atcacgttta aatgacacca cctgtctgac
    40801 cggataccag taacttaccc atcgccagcc gtcaaactct ggtgtactgc tggtttgcat
    40861 attgatttct gcatcgccgc ttaccagctg caagagaaac catttttgtt tttggccgat
    40921 acaaaccggc ttcgtgtccc aacgcaccaa acgtttcggt aatttgtagc gcaaccagtt
    40981 acgcgttgaa gcaaggattc gaacgtcttt gcggcttaat cctacttctt caaacaattc
    41041 acggtacatc gcctgctctg cggattctcc ggggttgatt ccgccttgcg gaaattgcca
    41101 ggagtgctga ccaaatcgcc gggcccacat tacctgcccc tggcgattac aaatcacgat
    41161 acctacgttt gggcggtagc catcgtcatc aatcaccgga ctacctcaaa ataaagcttt
    41221 atatacgaat gattgtttca tactccagga agacggtaaa ccactctctg cagggcatta
    41281 cacactaata acaattgaat aactcacagt tatgtgcaga gttataaaca gaggaagggg
    41341 tggatagccg tttttattca ccttttctgt ggatagagtt gtgaagaact acggaattac
    41401 tacgggaaaa cccggagcat tctgaataag cccgatatag acattttaaa atattcatta
    41461 ttagtcaatg cattacatgt aatttgagta atgaaaaaga gtaattcgtg acccaggtca
    41521 cacctctcat ttacgggttg gcgaaagatc aaccaatgcc gtgtttatcc acagaatgtg
    41581 ccactaagtt aagcactgaa ccactaaaaa ctggagtttc gtcgcacgtc aaggctgtaa
    41641 atgaaaacag tagtggaggt ttttcacagt tatcccagct ttctgtggat aacatggtgt
    41701 aagatcctgt ttattttcag tgaccagatt tggaaaacct attgcagtgt tgcgcaactc
    41761 gattaccggc agcctaaaaa gcaatataaa tcagagaatt gaacaacaca tgtggaaaaa
    41821 gttacactgc gaatattcgg cacataattg ctgtttgttt tttaaccaag gtatcatgac
    41881 atgtcccaac ctcgcccact gctctctcct cccgaaactg aagaacagtt gttagcgcaa
    41941 gcacagcaac tttctggtta tacattggga gaactggcgg cacttgacgg gctggttacg
    42001 ccagagaatt taaaacgcga taagggctgg attggcgtgt tactggagat ctggctaggt
    42061 gccagcgcag ggagtaaacc tgagcaagat tttgctgctc tgggcgtgga acttaaaact
    42121 atccctgtgg atagtcttgg tcgtccgctg gaaacaacat tcgtttgtgt tgcccggtta
    42181 acgggtaata gcggggtgac ctgggaaacc agccatgtgc gccacaagct caaacgcgta
    42241 ctgtggatac cggttgaagg cgagcgcagc atcccgctgg cgcagcgtcg cgtaggatca
    42301 ccgttgctgt ggagcccgaa tgaagaggaa gaccgacagc tacgcgaaga ctgggaagaa
    42361 ttaatggata tgattgttct cggtcaggtt gagcggatca ccgctcgtca cggggaatat
    42421 ttacagataa gaccgaaagc agcgaatgcg aaagcgctta ccgaagccat tggtgcccgg
    42481 ggcgaacgga ttctgacgct gccacgcggc ttttatttga agaagaattt caccagtgca
    42541 ctactggccc gtcattttct gatccagtag ccatcgcttt gacctgccgc tttccgggca
    42601 tataattacc gcttcatttt tttggcaggg ctttttagat gttatttgca tggataaccg
    42661 atcctaacgc ttggcttgcg ctcggtacgc tgacgctgct ggagatcgtt cttgggatcg
    42721 acaatattat tttcctttct ctggtggtgg caaagcttcc cacagcacaa cgtgctcatg
    42781 cgcgccgtct ggggttggcg ggagccatgg ttatgcgtct ggcgctgctg gcatcaatct
    42841 cttgggttac gcgcctgaca aatccgcttt ttacaatatt cagtcaggaa atttctgccc
    42901 gtgatttgat tctgcttctg ggtggcttgt tccttatctg gaaagccagc aaggaaatcc
    42961 acgaatccat tgaaggtgaa gaagaagggc tgaaaacacg cgtttcatca ttcctcggcg
    43021 ctatcgtgca gattatgctg ctggatatta tcttcagcct cgactcggtg attaccgctg
    43081 tgggtctgtc agatcacctg tttattatga tggcagccgt ggtgattgcc gtaggcgtga
    43141 tgatgttcgc cgcgcgctcg attggtgatt ttgtcgaacg ccatccttcg gtaaaaatgc
    43201 tggcgctctc tttcctgatt ctggtgggct ttaccctgat tctggaaagt ttcgacatcc
    43261 acgtaccgaa aggttacatc tacttcgcga tgttcttctc tattgcggtt gaaagcctca
    43321 acttgattcg caacaaaaag aatccgctct gatactccgt acgctctcct gcgggagggc
    43381 gtactcactc cttttaccct cacagtttaa gattttcctg ctttcaagat atataacgtc
    43441 ggtttataaa cagactatta tcataggtag gccaaatgag gataaccgga tgaaaaaatg
    43501 ggcagtaata atttctgcag tcggactggc gtttgctgtt tccgggtgtt ccagtgatta
    43561 cgtcatggcg accaaagatg gccgtatgat tttgaccgat ggaaaacctg aaattgatga
    43621 tgataccggg ctggtgagtt atcacgatca gcaaggtaac gcgatgcaaa ttaaccgtga
    43681 tgatgtttcg caaattattg aacgttaaca aataaggtca gcatccggct ggccttaaga
    43741 tttttctctt tcccttttcc cttccctctg ccatttttat attccttatg tcgtgattat
    43801 aaaaaggaaa cggctatgca atatcaccgt ataccccaca gttcgctgga agtcagcacg
    43861 ctggggcttg gcacgatgac gtttggtgaa cagaacagcg aagccgacgc ccacgcacaa
    43921 ctcgactatg ccgtcgctca gggcattaac cttatcgacg ttgccgaaat gtacccagta
    43981 cctccgcgcc ccgaaacgca agggttaacc gaaacctacg tcggcaactg gctggcgaaa
    44041 catggcagcc gcgaaaagtt aattatcgcc tccaaagtga gcggaccgtc gcgcaataat
    44101 gacaagggca tccgcccgga tcaggcgctg gatcggaaga atatccgcga agcgctgcat
    44161 gacagcctca agcgcctaca gactgattac ctcgatcttt atcaggtgca ctggccgcag
    44221 cgcccgacca actgcttcgg caaactcggt tatagctgga cggattctgc gcctgcggtt
    44281 tcgctgctgg atacgctgga cgcactggca gagtaccaac gcgcgggaaa aattcgttat
    44341 atcggcgtgt cgaacgaaac tgcatttggc gtaatgcgct acctgcatct ggcggacaaa
    44401 cacgatctgc cgcgtattgt caccattcag aacccttaca gtctgttaaa ccgcagtttt
    44461 gaagtaggtc tggcagaagt cagccagtat gaaggggtcg aactgctggc ctattcgtgc
    44521 ctgggtttcg gcacgctgac cgggaaatat ctcaatggtg caaaacccgc tggcgcacgt
    44581 aatacgctct ttagtcggtt cacccgctat agcggtgagc aaacgcaaaa agccgtcgcg
    44641 gcgtatgttg atatcgccag acgtcatggc cttgatcctg ctcagatggc gctcgcgttt
    44701 gtacgccgtc aaccgtttgt tgccagcact ctgctgggcg caaccacgat ggatcagctg
    44761 aaaactaaca tcgaaagttt gcatctggag ttaagcgaag acgtattagc tgaaattgaa
    44821 gcggtacatc aggtttatac ttatcctgca ccataaagat taacgccggt taaaaccggc
    44881 gttaaatatt aatgacggcg ctgccagatc cacagcgccg ttattgccag cgcaaatagc
    44941 gcaccgaagc caatgccaat gggcacgacc gggatgccta ccattaccgc cagcgagtaa
    45001 atgcccagca tcaacaacat ggcgctgttt tcgccaaggt tttgtactgc aatcgcattc
    45061 cccgccccga cgcttttttt accccgctcc tgtagcaacg cattgagcgg aacgacaaaa
    45121 aagcccccca gcacgccaat cagcatcaac aaggcatagg ctggcagcag ctcgtgttgc
    45181 agggagaaaa tcggtaccac cacgccaatc aaaatcccgg ctggcataca gcgtgacacg
    45241 gtttccagcg taaccagctt cgctgccgca cctgcgccaa ccacgatacc aatcgctacc
    45301 atcgcgttga gataggtcgg cgtagcgtta tcggtaatgc ccagcgccac cggtacccac
    45361 agcaccaaca ggaaacgcag cgtgacaccc gctccccaga ataaactggt gcccaccagc
    45421 gaaaaacgcg tttcaccatt gcaccatagc gaggtgcagg cattcaggaa actgcgggtc
    45481 atgttgatga gattccagga ctgccccgga cgcgccgcca gtttgggaat gtagatattg
    45541 gcaacgaccg caccaccgta ggccagtgcg catgcggcca gagcgaccag gacatgccag
    45601 tcagccagca caccaccggc tacggaaccg agtaaaatcg ccgctatggc agaagcttcc
    45661 attaaaccgt tagcttttac taacttacta cccgtggtta attcgccgag aataccgtat
    45721 ttcgccggtg aataggctgc agcaccaaca cccaccagcg tatagccgag aaacggattg
    45781 ataccaaagc agatactggc tgcgcccagc agcttcaggc cgttggcaaa catcatcacc
    45841 cggcctttgg cgaagctatc cgccacctgc ccgacaaacg gcgccaaaag aatgtaagca
    45901 cctacaaaca ccatttgcag gatgggctgg ctccactccg gatagaactg cgctttcagt
    45961 aacgccagag tggcaaacag tagcgcgtta tcgccaaacg cagagagaaa ctgcgccacg
    46021 ataaccgctt tcatcccctt cgaccacaac gaagtgttag tgtgcactga ctcactcatc
    46081 gtgttgttcc gcttcgtcta cccagctttt caacgtgaca aagtcaggtt tgccgctgcc
    46141 aagtaatggc atctgtttca gatagcgaat atcgcgcggt acagcaagct ccggcacgcc
    46201 gtgctcgcgg gcatactgtt gcaacttatc gcgcgtcagt tcgttatctg tggtgaaaag
    46261 caccagtgcc tcgcctttgc tggcatcgct cttaatcgca gtggcatgga ctttatctgg
    46321 cgaaacacca agtgccagtt gttccaccat ttccagcgac accatttcgc ctgcaatttt
    46381 ggcaaagcgt tttgcgcggc cctgaatctg cacaaagccc tgctcgtcaa aacgcacaat
    46441 atcgccagtg tcataccagc cgcgctccat ttcgccgcga acattctcgg cggtgggcac
    46501 ttccagtaca cctggcttct ccacccgcag atagccgttc attatgttcg gccctttcag
    46561 ttgcaggcgt ccgccctctt cgataccagg gaccgacaac aggcgtgcat ccattcctgg
    46621 tagaatacgc cctaccgtac cgggtttcgc cgccatcggt acgttgatag aaacgacagg
    46681 cgcgcattcg gtcacaccgt agccttcaag gatgcgcagg ccaaatttat cctgccaaag
    46741 ctgtttggta ctttcttgta gtttttctgc gcctgccacc acatagcgta gacgatagaa
    46801 gtcatacggg ttggcgaaac gcgcgtagtg accgaggaaa gtcgaggtac cgaacaacac
    46861 ggtgcaactg cggtcataca ccaagtccgg cacaatgcgg taatgcagcg ggcttggata
    46921 aaggaacact tctgcacctg taagcagtgg cgtaaacagg cctaccgtca gcccaaagga
    46981 gtgaaacagc ggtaatgccg acataaagcg atcgttggtg gtgaagtcgg caatcgtttt
    47041 aatctgctcg acgttcgcca gaatgctttt atggctatgg acgacgcctt ttggatggcc
    47101 ttcagaaccg gaggtaaaga ggatcagcgc ctcttcttcc ggctgctgtt ttacctgcgc
    47161 caggcgcggc atcagcaaat gagcgaagat ccatactttg tcggcagtgg tgacatctgc
    47221 ttttaaatct tccagataga cccagcgcac ctgagtaagt tgctccggca gatgccagag
    47281 tttgccttta tcgagaaact ggcgggaagt gaagatggtt ttgatttcag ccgccgtaat
    47341 agcactggtc agccctttta ccccggcagt gtagttcatc attgcgggaa tgcggcgacg
    47401 cgcgatggcc ccaaaaatca ctgccgcact gatgcctgca ttgggcagca ttaagccgat
    47461 gcgttcgcct tcaacactgt atttttcaag gatgcgtcca acaaacagcg ttttcgtaag
    47521 caatttgcga taggagtctg gggtaaagtt gacgtcttcg acacatttct tcccggctcc
    47581 gaagcggtac attgcactca gtaaagattc gtacagagtt tcacgcgggc gcaccgccat
    47641 acgcgcttcc atcattattt gatgcagcat ttcgccagcg attttgcgac ggtcacgggc
    47701 acgcggcgca tccggcatcg ccacctgcgt tggaggcaaa atatgcagag taatttgcgg
    47761 gaacaagcga cgtttaacca gacctttcag gcggctgaag tgcgtaagtt ccgccccttc
    47821 aatacgcaca ggaataaccg ttgcaccaga cttcgccgcg acaaaacccg cgccatcgta
    47881 gattttcatc agcgagcctg tagtggtgat gcgtccttca gggaaaatca ccactggtcg
    47941 gccctgttcc accagacgta ccagatgttt aatagccata ggttgcgtcg ggtcgagagg
    48001 aacaaagtcg ataaatgatt tcagccaacg catataccac tgttggctta ttgaggtgta
    48061 aacggcaaac actggacgca caggtaaaaa cagtccaagc aaaatgccat caataaaaga
    48121 gacgtgatta ggcgtaatta gtacgcgctc gcccttcagt gcctgggtgt cacccgtaac
    48181 gcgaacgcga tacaaaacac ggcacaaatt tcgaaaaaag ctaaaaagca ttccaactcc
    48241 ctttgctctg attcagtaaa agcgaatgga gggagattac acgagataaa gaacgcgagc
    48301 gacagtaaat taggtgcgaa aaaaaacctg cgcatccgcg caggttggtg caagagacag
    48361 ggtacgaaga gcgtaccgaa taatctcacc aatcaatacc tctgggatct tgattgtggt
    48421 ctgcacgacg actcttcgcc agcgagaaaa cgcaaaggaa tgaagggaaa tgcaacgagg
    48481 tgtgtaaatt gtcggttact gttacagatt gatgaccggc aaaaaaaaac ctgcgcatct
    48541 gcgcaggctg gtgtaattca tgtgctcaac acgaagttga cttcacctat caatacctct
    48601 gggatcacca ctttagcaac ctgaagccaa acgccaccag cggtcaatcg caacagcgtt
    48661 tcgcaaagtg taaccaaagg tttgcaatct ctttttttgt cttacttatt tgcgtttggc
    48721 tcacattccc acgatgaaaa cacgccaccc cttgaaccaa cgggcgtttt ccgtaacact
    48781 gaaacaatgt aagcgtttac ccactaaggt attttcatgg cgaccataaa ggatgtagcc
    48841 cgactggcag gcgtttcagt cgccaccgtt tcccgcgtca ttaataattc acccaaagcc
    48901 agcgaagctt cccggctggc tgtgcatagt gcaatggagt ctcttagcta tcacccgaac
    48961 gccaacgccc gtgcgctggc gcagcagacc actgaaacgg tcggtctgat cgttggtgat
    49021 gtttccgatc cgtttttcgg tgcaatggtg aaagcggtcg aacaggtggc ttatcacacc
    49081 ggtaatttct tattgattgg caacggttac cacaacgaac aaaaagagcg tcaggctatt
    49141 gagcaactga tccgccatcg ctgtgctgcg ttggtcgtcc atgccaaaat gatcccagat
    49201 gccgatttag cctcattaat gaaacaaatg cccggtatgg tgctgatcaa ccgtatcctg
    49261 cctggctttg aaaaccgttg tattgctctt gacgatcgtt acggtgcctg gctggcaacg
    49321 cgtcatttaa ttcagcaagg tcatacccgc attggttatc tgtgctctaa ccactctatt
    49381 tctgacgccg aagatcgtct gcaagggtat tacgatgccc ttgctgaaag tggtattccg
    49441 gccaatgatc ggctggtgac atttggcgaa ccagacgaaa gcggcggcga acaggcaatg
    49501 accgagcttt tgggacgagg aagaaatttc actgcggtag cctgttataa cgattcaatg
    49561 gcggcgggcg cgatgggcgt tctcaatgat aatggtattg atgtaccggg tgagatttcg
    49621 ttaattggct ttgatgatgt gctggtgtca cgctatgtgc gtccgcgcct gaccaccgtg
    49681 cgttacccaa tcgtaacgat ggcgacccag gctgccgaac tggctttggc actggcggat
    49741 aatcgccctc tcccggaaat tactaatgta tttagtccga cgctggtacg tcgtcattca
    49801 gtgtcaactc cgtcgctgga ggcaagtcat catgcaacca gcgactaacc gcagttaaag
    49861 caattccagc gccagtaatt cttcgatggt ctggcgacgg cgaatcaacc gcgcctgacc
    49921 attatcaaac agaacttctg gtaacagcgg acggctattg tagttggatg acattgatgc
    49981 gccatatgcc cctgtatcat gcagtaccag ataatcacct gccttcactt ccggcaaggc
    50041 gcgggtttca acatttcccc cttcctgctg ggtaaagaca tcgcccgatt cacataacgg
    50101 cccggcgacg acggtttcca ccgttggcgc gtgttccaga gaacgaccat cagctgccag
    50161 ggcactgata tggtggtaac taccgtacat tgccgggcgc atcagatcgt tgaacccggc
    50221 atcaaccagc acaaagtggc ggctacccat ttgtttgacg ctccgcacct gagtaattaa
    50281 tacgccagac tgcgctacca ggaagcgacc cggttcaatt tccagtttca cagggtggcc
    50341 caaatggcga gcgatttgct cacgcgcggc attccacaga ccataataat gttcggtatc
    50401 aaccgcctct tcaccctgtt gataaggaat agaaagccca ccgcccgcag aaatagcctg
    50461 taaatcctga ccgaattcga taacctgacg caccatagca ccacacacct gctccagatg
    50521 ggcataatca acgccagaac caatgtgcat gtgaatgccg actagctgca gatgatgacg
    50581 ttgtatcacg tccagtgcgg cgggcagatc ggtgtaccag ataccgtgct tgctgttttc
    50641 gccaccggta ttggtttttt ggctatgtcc gtgaccaaac cccggattaa cgcgcagcca
    50701 tacccgatgc cctggcgaaa cctggcccag ttggtcgagc gtatcaacag aacccgcatt
    50761 caccggaatt tgcaattcac tgacgcgttc aagcgtcgcc tgatcgataa catctgccgt
    50821 aaaaacaata tcatcggggt gcgtttgcgg attgtaaccc gccgccaacg cacgctctat
    50881 ttcgcctaac gagacggaat ccactttcac gccctgctca cgcattaagc gcaaaatatg
    50941 aatattggaa caggctttct gtgcaaagcg caccacttca aactgtttca gcgctgcaat
    51001 ctgccgacga ataatttgcg catcgtagac ccacaccggg cagccaaatt cagcgggcaa
    51061 acgcagcaga ttttcggcgg tgagatcggt atcggtgctg aacagtgaat gtggcataac
    51121 aaactccaga tgagtgcttt tttatgatta cgccacatca taaaaagaat aaaaaatatc
    51181 gatttatgtc gagtctatgc aaaaatgata tgaattaccg gattgcgaga gagcgctaat
    51241 ggccgccgtt aacttacgtc atattgaaat ttttcatgcg gtaatgaccg ccggaagcct
    51301 gactgaggca gcacacctgc tacacacctc acagccaacc gtcagccgcg aactggcgcg
    51361 ctttgagaag gtgatcgggc tgaaattgtt tgagcgcgta cgtggacgat tacatcctac
    51421 cgtgcaagga ctgcgtctgt ttgaagaagt gcaacgatcc tggtacggac tggatcgcat
    51481 tgtcagcgcc gcagaaagtc tgcgcgagtt tcgccaggga gaactgtcta ttgcctgcct
    51541 gccggtcttt tcgcaatctt ttttaccgca gctcctgcaa ccctttctgg cacgttatcc
    51601 cgatgtcagc ttaaatatcg tgccccagga atcaccgcta cttgaagagt ggctctcggc
    51661 ccagcgtcat gatttaggac tcactgagac actccatacg cctacgggaa cagaacgtac
    51721 cgaattactc tctttagatg aagtgtgtgt gttacctccg ggtcatccgc tggcggtaaa
    51781 aaaggtatta acgccggatg attttcaggg tgagaattac atcagccttt cccgtactga
    51841 cagctatcgc cagttgctgg atcagctatt tactgaacat caggttaaac gacgcatgat
    51901 cgtagaaacc cacagcgccg cgtcagtctg cgcaatggta cgggcggggg taggtatttc
    51961 ggtggttaac ccgctcaccg cactagatta tgcggcaagc ggtttagtgg tgcggcggtt
    52021 cagtattgcg gttccgttca ccgtcagcct gatccgcccc ctgcaccgcc cgtcatcagc
    52081 gctggttcag gcgtttagtg ggcatttaca agcggggtta ccgaaactgg tcacttctct
    52141 tgacgctatt ttgtcgtcag ctacgacagc ataaaagcga cagcatcctc ggcatggatc
    52201 gccgcggtat caaacacagg caaaacactg cgctcttctg gcaccagtaa accaatttct
    52261 gtgcagccaa aaatgacgcc ctgtgcgccc tgttctgcaa ggcgagcaat cacttgcgca
    52321 taataagcgc gtgacgcttc ggtaaattgc cccagacaca gttcttcaaa aataatctga
    52381 ttaattttcg cccgttcatc cgcttcagga ataagacaat tgatggaaaa ttgttccgtc
    52441 agccgcccgc gataaaaatc ctgttccatg gtgtaacgcg tacccagcag cgccacacga
    52501 gtcattccgg ccccggtaat tgcacgtccg gtggcatccg caatgtgtaa gaaaggcaga
    52561 gtgcaacgtg actcaatggc atccgccact ttatgcatcg tattggtaca taacacaata
    52621 ccttctgcgc ccgcccgctg taagccaagc gccgcctcag ccagaatgtc cccggtttta
    52681 tcccattccc cgcgacgctg gcactcttct atttcatgaa aatcgacgct atgtagcagc
    52741 atttgcgcag agtgaagccc accaagccgc tgtttaatgc cttcatttat cagacgatag
    52801 taaggaatgg tggattccca gctcattcct cccagcaaac caattgtttt cattcctctc
    52861 tcctgatgtg tgttaccgca acaaggctga ataaggaacg agacaaacgc ctcaacggcc
    52921 aggtgcccaa tctctattaa cgaaaaaagg gccggatgta cagcacatcc ggcccgtgaa
    52981 atcagacgcc gatatttctc aacttctcgc ctgccatcag tttgcgttcg atatgttcca
    53041 gcgtgacatt tttggtttcc ggaatgagcc agaaagtaat acccacaaac gcaatgttca
    53101 gcgcagtgta gagccagaac gtaccggcag cgccaatgct atcaagcagt gtcaggaagg
    53161 ccgcgccgat aatcatattc gacacccagt ttgtcgtcgt cgaacaggta ataccgaaat
    53221 cgcggcattt cagcggctga atttcagagc acaggatcca caccactggc gcggcgctca
    53281 tcgcataacc ggcaatacac atcatcgtca tgccaacaga gagccaggac aagccactgg
    53341 aagccgtacc gttatcaaac tgcatcaggc aatagcccag caccagagtg cctaacgcca
    53401 tcacgctgaa accaattttc agagccggtt tacgacctgc tttatctacc gtaaagaccg
    53461 caatgaaggt cgcgaacata aaggtcagtc ccacgaccag agtcgcaatc atctgttgtt
    53521 ctgtggtcgt aaagcccgcc attttgaaga tacgcggcgc gtagtacatg atgatgttca
    53581 taccggtaaa ctgctgcatc gcctgcaaca acataccgag aaacacagca cgacggacgt
    53641 tacggttgat cttaaacagt gcccaaccgc cctgttttaa cttcaggctt tcacgaattt
    53701 cgttgagttc ttctcgcgct ttttccgacg tatcgcgcag catacgcaac acttcttccg
    53761 cctcaatatg acgccccttt tccgccagcc agcgcgggct atttggcagg aagaccacca
    53821 gaataatcag cagaactgct ggtaaagcaa gaacccccaa cattgcgcgc cagttaccgc
    53881 tataactgaa cgctgtatcg gataaaaacg ccagcacgat gccgagtgtg accatcaact
    53941 ggtacatact gatcatctta ccgcgaacgt tttcacttgc catttcagaa agatacagag
    54001 gagcggtgta agacgcgatc ccgacagcaa tgcccagcac cacacgagcg gcgattaaca
    54061 tctctacgct ggtcgcaaaa gcggacccta tagaaccgag tacaaacagg atggcccccg
    54121 ccatcaggct gtatttacgc cccaggcgga acgacagcca accattaaac agcgcaccaa
    54181 ttgctgcgcc gagcatcatg ctactaacca cccattcctg caaacgactg gtcagcacaa
    54241 agtgatcggt aatgaacggc aacgctccgg cgattacgcc gatatcaaga ccaaataaca
    54301 atcctgcgac cgcagcagct accgaaacaa acatattcat acgccgcgta tcacgcaaag
    54361 aacgtggcgt taaagcagat tccgtattga tagtaaccat tttttcctgc cagcagagag
    54421 taagacattg tgaaaaaata cgtgaacaat tcacacaggt gtcaggtcgg aaacagcata
    54481 aatatggatt aaattgctgc gacatgccgt tatgtgatgg atattccaat tttaaaatta
    54541 agttgaatta ttgagattat tattaacctc ctaattttaa agcagataaa attcataaag
    54601 ttcattaatt gataattgat atggattatt tcataaccat gatatggatt atgatgatct
    54661 agagatataa aaaaccctgc catgcggcag ggtcataaaa gtaagaggaa tgaattaacg
    54721 cgccagccaa ccgccatcca cagcaatggt ataaccattt acataatctg aagcgctgga
    54781 ggcaagaaac actaccggcc ccatcaggtc actcggcagt ccccaacggc cagctggaat
    54841 gcgatcgaga atttccgcgc tacgttgttc atctgcccgc agttgttggg tattgttggt
    54901 cgccatgtaa cccggagcta tcgcattaac attaatgttg tgtttagccc attcgtttgc
    54961 catcaatcgc gtcacaccca tcacgccgct ttttgatgcg gtataagaag gcacacggat
    55021 cccgccctgg aaggagagca ttgacgcgat attgataatc ttgccgccat tgccttgcgc
    55081 gataaagtgt ttcgccgctg cctgagacat gaagaatacg ctcttgatat tcaggttcat
    55141 gacatcgtcc cagtcttttt cgctgaactc gagagcgtct tcgcggcgaa tcaatccggc
    55201 gttattcacc aggatatcaa tatgaccaaa ttcagctacc gcgcgatcca gcagtccagg
    55261 aatgccatca atctttcgca gatcggcggt caggcttaaa aaacgacgcc ccagcgccgt
    55321 gacctgctcg atggtttcag tcggttcaac gatgttaatg ccaacaatgt cacagcccgc
    55381 ttgcgccagc cccaacgcca tcccctggcc cagcccagta tcacaaccag tgacgaccgc
    55441 aactttacct tggagagaaa atgcacttaa aatcataaca acaccttatt cgtttatgcc
    55501 cacaactagc gcaaatcttt aacgcccaca tggtccatat cattaaagac ctggttttca
    55561 ccgaccatgc cccagataaa ggtataagct ttggttccga cgccggaatg gatcgaccag
    55621 ctcggggaga tcaccgcctg ctcgttatgc atcacaatgt gacgcgtttc ttgcggctgc
    55681 cccatcatgt ggaaaacgca ggcgtcatca tccatattga aatagaaata aacttccatc
    55741 cggcgctcgt gggtgtgaca cggcatggtg ttccacaagt tacctggagc cagctccgtc
    55801 agccccatac tcaattgaca ggtttccagt acatccggga caaaatattt gttaatcgtg
    55861 cgacggttac tggtgaggtt atcgcctaac gtgactggag atacttcgtc cggtgtgact
    55921 tttttggtgg gatacgtcgt atgcgcgggt gcgcaattgt aataaaactt cgccggagtg
    55981 gcggtatcaa tactggcaaa gacaacttct tttgcacctt taccaacata cagggcgtcg
    56041 cggtgaccga tttcatagca ttgaccatcg acagtaatcg taccggcacc gccaatattg
    56101 ataacaccta actcgcgacg ttcaaggaaa tagcttacgc cgagttgttt accaacttcc
    56161 ccgccaacgg aaaccgtttt agttatcggc ataatgccgc caacaataat tcggtcaatg
    56221 tggctgtaaa ccatggtgta ctcatcggcg acaaatacct tttcaaccaa aaattcattg
    56281 cgcagccctt gggtatccag cgtttttgcg tgcgcaccgt ggatgctctg tcttacgtcc
    56341 acatcaacct ccgataatca gtgaagcgtg aaaatagaaa acgatagtga aacactgttt
    56401 cgttttctct ataaagggat gatatgccca tgggctaggg ttttcaatta aaaataaaac
    56461 aatgttttat ttttaattta aggattaaaa aaagtgcagt gtcgatcacg aatatgtcat
    56521 gagaatggaa aaatgaggga gcccagaaaa tgtatgtcga cttgccagcc atcattattc
    56581 aacagattaa tgacggcaga tatttatcgg taaggaggaa atgcagagct actcgtcacg
    56641 ttcaatggtc aatgccacgc cctggccccc gccgatacaa agcgttgcca gccctttgcg
    56701 ggcattacgt ttcaccattt catgaaccag agaaaccagg attcggcaac cggaagcgcc
    56761 tatcgggtga ccgagtgcga tcgcgccacc attgacattg acccgacgct catcccactc
    56821 aagcatcttg ccaaccgaaa gcgcctgtgc agcaaacgct tcattagcct cgataagatc
    56881 gacatcagcc aactgccagc ctacacgctc caggcaacgg cgggtcgcat acaccggcgc
    56941 aattcccatc aatgccggat ctacaccaac gctggcaaat gcgcggatgc gggccagcac
    57001 gggtaaattc aacgctcgtg ctttggcttc gctcatcatc attaccgcag ctgcgccatc
    57061 gtttatggat gatgcattcc ccgcggtcac agaaccgaga ctatcaaatg aaggatttaa
    57121 acgggctaaa ccttctgcac tggcgtcagt gcgtggctgt tcatcggtat caacaaccaa
    57181 cgtctgaccg ttactttgga tcattaccgg gacgatctca tctttaaatc gtccggcgtc
    57241 aatcgccgct cgcgcttttt gttgcgagct aagtgcgtaa gcatcctgca actgacggct
    57301 gatgccatat tcgcgagcca gattttcggc ggtgacacca atatgataat cattgaaggc
    57361 atcccacaac ccatcatgca ccagactgtc aaccaactgg ctattgccaa gctgttcacc
    57421 ggtgcggcta tcagtcagaa catgtggtgc gcggctcatg ttttcctggc cacctgcgat
    57481 gacaatatca gcctcgccac actgtatcgc ctgagtagcc agatgcagtg ctttaagacc
    57541 ggaaccgcaa acgtcattaa tcgtgattgc agaaacgctg ttaggaagac cacctttaat
    57601 agccgattgc cttgccggat tctgccctgc ccctgcagtc aacacctgac caagaattac
    57661 ttcatccacc gcatatgcag gaacgccggt acgttctatt aacgctttca cgaccagact
    57721 accaagttcc acggcggaat gacccgctaa cgcaccacga aagcagccga taggtgtccg
    57781 taacgccccg acaatcacaa cgtctttcat cacaacctcg caccaattaa cagtgcaata
    57841 gtaaatgatt gttaattaca gttatcttaa ttgtttaaaa aaagtgattt ttatcacaaa
    57901 ggaaatatgc ctgagcagta gtcagagtca taactggcac gtaaggtttg caaccactaa
    57961 cccaccaata gatgggtaga tagggcgtta atctcccata cttaacttgg tttatggtaa
    58021 attgccctcc attttgttta atttgtagat gatacgttca gataatgtct aatatttggt
    58081 ctaaagaaga aactctgtga agtttcgcgc tctatggcac agccgttgat gcaggcacgc
    58141 tattccttcc tattcagtta ggttcggcag gggctgtggt cctgtttatt actgctctgg
    58201 tcgcctggcc tttaacatat tggccacata aagccttatg ccagttcatc ctctcatcaa
    58261 aaacatcagc aggtgaaggg ataacgggcg cggtaacaca ctactatggc aagaagattg
    58321 gtaatctgat taccacgctg tacttcatcg ccttttttgt cgtcgtgttg atatatgcag
    58381 tggcaattac caactcactt acggaacagc tggcaaagca tatggttatt gatcttcgca
    58441 tccgtatgtt ggtgagtctg ggtgttgtat taattctgaa tctcattttt ctgatgggac
    58501 gtcatgccac tattcgggta atgggatttt tggtattccc attgattgcc tatttcttat
    58561 ttctttccat ttaccttgtc ggtagttggc aacctgatct attaacaacc caggtagagt
    58621 tcaatcagaa tacccttcac cagatatgga tatcgattcc cgtgatggtt ttcgccttta
    58681 gccatacgcc cattatttct acgtttgcca tagacagacg tgaaaaatat ggcgaacacg
    58741 ctatggataa atgcaaaaaa attatgaaag tcgcttatct catcatctgc ataagtgtac
    58801 tgttctttgt ctttagctgc ctgctttcta ttccatcttc gtatattgaa gcggctaaag
    58861 aagaaggggt caccatttta tcggcgcttt ctatgctgcc gaacgcccca gcatggttgt
    58921 caatttccgg gattattgtc gcagtagttg cgatgtcgaa atcattcctg ggtacgtact
    58981 ttggcgttat tgaaggtgcc acagaggtcg tcaaaacaac actacagcag gttggtgtaa
    59041 agaaaagtcg tgcatttaac cacgcactat caattatgtt ggtatcgctg attaccttca
    59101 ttgtttgttg cattaacccg aacgcgattt cgatgattta cgcgatcagc ggcccgctca
    59161 ttgccatgat acttttcatc atgcctacgc tgtcaacgta tctcatcccg gcgcttaaac
    59221 cctggcgttc catcggaaat ctgattacgc tgatcgtggg tatcctgtgc gtatcggtaa
    59281 tgttctttag ctaatatccg ttgtggatgg ttcaaaattg ctacaatttt gaaccattcc
    59341 atttttcata atattcattt gttattattc cgccctatca cttctctcgt catatcctag
    59401 aattatcagt tagttaaaca cacaaagaga tctgaacatc ttaaataaga ctatttaaga
    59461 tgcataactt agattcgcaa gatatcttct ggtcattcaa aaacaatttg cgatatagat
    59521 attctcatac catgagatag ttatctggac cacgttcgac catgcaagat taaatcagtg
    59581 aaacataatc atatttaata acgagaaatg catttttaaa tatcttaatc agaattatag
    59641 caaatacaga ttaatcgcaa tttttcccag caaaattaca aaatcaaggt gtattgacat
    59701 catcaatcac caatgaaatt atgcaattat atacggatag ggaggttctt aacatggggc
    59761 tttgtagtcg ttataaaagt cttacatgca atagttgctc aatgcattgc caaataatgc
    59821 cagaagagtc accgcgtttg cagtactgcg caaactcgtg ttttgatctt acccagcaat
    59881 agtggacacg cggctaagtg agtaaactct cagtcagagg tgact
//
LOCUS       DAEBXN010000009        59734 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.009, whole genome shotgun sequence.
ACCESSION   DAEBXN010000009 DAEBXN010000000
VERSION     DAEBXN010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 59734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59734
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.009"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(141..2588)
                     /gene="glgP"
                     /locus_tag="KJL99_000543"
     CDS             complement(141..2588)
                     /gene="glgP"
                     /locus_tag="KJL99_000543"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HBD4435402.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2607..4040)
                     /gene="glgA"
                     /locus_tag="KJL99_000544"
     CDS             complement(2607..4040)
                     /gene="glgA"
                     /locus_tag="KJL99_000544"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HBD4435403.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHVHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KSQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(4040..5335)
                     /gene="glgC"
                     /locus_tag="KJL99_000545"
     CDS             complement(4040..5335)
                     /gene="glgC"
                     /locus_tag="KJL99_000545"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HBD4435404.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5353..7326)
                     /gene="glgX"
                     /locus_tag="KJL99_000546"
     CDS             complement(5353..7326)
                     /gene="glgX"
                     /locus_tag="KJL99_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HBD4435405.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGNSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPSGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLNWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITVVWQGPAHGLCVFQR"
     gene            complement(7323..9509)
                     /gene="glgB"
                     /locus_tag="KJL99_000547"
     CDS             complement(7323..9509)
                     /gene="glgB"
                     /locus_tag="KJL99_000547"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HBD4435406.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SIHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9782..10885)
                     /gene="asd"
                     /locus_tag="KJL99_000548"
     CDS             complement(9782..10885)
                     /gene="asd"
                     /locus_tag="KJL99_000548"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBD4435407.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            11077..11670
                     /gene="yhgN"
                     /locus_tag="KJL99_000549"
     CDS             11077..11670
                     /gene="yhgN"
                     /locus_tag="KJL99_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HBD4435408.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11727..13067)
                     /gene="gntU"
                     /locus_tag="KJL99_000550"
     CDS             complement(11727..13067)
                     /gene="gntU"
                     /locus_tag="KJL99_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HBD4435409.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGAATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(13071..13598)
                     /gene="gntK"
                     /locus_tag="KJL99_000551"
     CDS             complement(13071..13598)
                     /gene="gntK"
                     /locus_tag="KJL99_000551"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HBD4435410.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDSAFAMQRTNKVSLIACSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13737..14732)
                     /gene="gntR"
                     /locus_tag="KJL99_000552"
     CDS             complement(13737..14732)
                     /gene="gntR"
                     /locus_tag="KJL99_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HBD4435411.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14956..15651)
                     /gene="yhhW"
                     /locus_tag="KJL99_000553"
     CDS             complement(14956..15651)
                     /gene="yhhW"
                     /locus_tag="KJL99_000553"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HBD4435412.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSCANYYDPNFMGFSALRVIN
                     DDVIEVGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15774..16811)
                     /locus_tag="KJL99_000554"
     CDS             complement(15774..16811)
                     /locus_tag="KJL99_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBD4435413.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKTFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            17144..17632
                     /gene="yhhY"
                     /locus_tag="KJL99_000555"
     CDS             17144..17632
                     /gene="yhhY"
                     /locus_tag="KJL99_000555"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBD4435414.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(17691..19433)
                     /gene="ggt"
                     /locus_tag="KJL99_000556"
     CDS             complement(17691..19433)
                     /gene="ggt"
                     /locus_tag="KJL99_000556"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HBD4435415.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDIKKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            19553..19993
                     /locus_tag="KJL99_000557"
     CDS             19553..19993
                     /locus_tag="KJL99_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HBD4435416.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            complement(19980..20723)
                     /gene="ugpQ"
                     /locus_tag="KJL99_000558"
     CDS             complement(19980..20723)
                     /gene="ugpQ"
                     /locus_tag="KJL99_000558"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBD4435417.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QHAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(20720..21710)
                     /locus_tag="KJL99_000559"
                     /pseudo
     CDS             complement(20720..21710)
                     /locus_tag="KJL99_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
     gene            complement(21712..22557)
                     /gene="ugpE"
                     /locus_tag="KJL99_000560"
     CDS             complement(21712..22557)
                     /gene="ugpE"
                     /locus_tag="KJL99_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HBD4435418.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(22554..23441)
                     /gene="ugpA"
                     /locus_tag="KJL99_000561"
     CDS             complement(22554..23441)
                     /gene="ugpA"
                     /locus_tag="KJL99_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HBD4435419.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSMVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(23539..24855)
                     /gene="ugpB"
                     /locus_tag="KJL99_000562"
     CDS             complement(23539..24855)
                     /gene="ugpB"
                     /locus_tag="KJL99_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HBD4435420.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAVFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTSVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGIKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(25252..25965)
                     /gene="livF"
                     /locus_tag="KJL99_000563"
     CDS             complement(25252..25965)
                     /gene="livF"
                     /locus_tag="KJL99_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HBD4435421.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(25967..26734)
                     /gene="livG"
                     /locus_tag="KJL99_000564"
     CDS             complement(25967..26734)
                     /gene="livG"
                     /locus_tag="KJL99_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HBD4435422.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(26731..28008)
                     /gene="livM"
                     /locus_tag="KJL99_000565"
     CDS             complement(26731..28008)
                     /gene="livM"
                     /locus_tag="KJL99_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HBD4435423.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWMFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLVVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRTQLKLKNGAAKGEQA"
     gene            complement(28005..28931)
                     /gene="livH"
                     /locus_tag="KJL99_000566"
     CDS             complement(28005..28931)
                     /gene="livH"
                     /locus_tag="KJL99_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HBD4435424.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYCVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(28979..30088)
                     /gene="livK"
                     /locus_tag="KJL99_000567"
     CDS             complement(28979..30088)
                     /gene="livK"
                     /locus_tag="KJL99_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HBD4435425.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINVKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            30512..30895
                     /gene="panM"
                     /locus_tag="KJL99_000568"
     CDS             30512..30895
                     /gene="panM"
                     /locus_tag="KJL99_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HBD4435426.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            complement(31094..32197)
                     /gene="livJ"
                     /locus_tag="KJL99_000569"
     CDS             complement(31094..32197)
                     /gene="livJ"
                     /locus_tag="KJL99_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HBD4435427.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(32468..33322)
                     /gene="rpoH"
                     /locus_tag="KJL99_000570"
     CDS             complement(32468..33322)
                     /gene="rpoH"
                     /locus_tag="KJL99_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HBD4435428.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMHGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(33567..34625)
                     /gene="ftsX"
                     /locus_tag="KJL99_000571"
     CDS             complement(33567..34625)
                     /gene="ftsX"
                     /locus_tag="KJL99_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HBD4435429.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVTQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(34618..35286)
                     /gene="ftsE"
                     /locus_tag="KJL99_000572"
     CDS             complement(34618..35286)
                     /gene="ftsE"
                     /locus_tag="KJL99_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HBD4435430.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            complement(35289..36785)
                     /gene="ftsY"
                     /locus_tag="KJL99_000573"
     CDS             complement(35289..36785)
                     /gene="ftsY"
                     /locus_tag="KJL99_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HBD4435431.1"
                     /translation="MAKEKRRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVAEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEDKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            36935..37531
                     /gene="rsmD"
                     /locus_tag="KJL99_000574"
     CDS             36935..37531
                     /gene="rsmD"
                     /locus_tag="KJL99_000574"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HBD4435432.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQHEAQGESDAD"
     gene            37521..37790
                     /locus_tag="KJL99_000575"
     CDS             37521..37790
                     /locus_tag="KJL99_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HBD4435433.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(37793..38152)
                     /locus_tag="KJL99_000576"
     CDS             complement(37793..38152)
                     /locus_tag="KJL99_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HBD4435434.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            38293..38919
                     /locus_tag="KJL99_000577"
     CDS             38293..38919
                     /locus_tag="KJL99_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HBD4435435.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            38993..41191
                     /gene="zntA"
                     /locus_tag="KJL99_000578"
     CDS             38993..41191
                     /gene="zntA"
                     /locus_tag="KJL99_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HBD4435436.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAT
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(41293..41538)
                     /gene="tusA"
                     /locus_tag="KJL99_000579"
     CDS             complement(41293..41538)
                     /gene="tusA"
                     /locus_tag="KJL99_000579"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HBD4435437.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            41759..42424
                     /locus_tag="KJL99_000580"
     CDS             41759..42424
                     /locus_tag="KJL99_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HBD4435438.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            42497..43054
                     /gene="dcrB"
                     /locus_tag="KJL99_000581"
     CDS             42497..43054
                     /gene="dcrB"
                     /locus_tag="KJL99_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HBD4435439.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(43058..44275)
                     /locus_tag="KJL99_000582"
     CDS             complement(43058..44275)
                     /locus_tag="KJL99_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD4435440.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            44409..45458
                     /locus_tag="KJL99_000583"
     CDS             44409..45458
                     /locus_tag="KJL99_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBD4435441.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            45513..46100
                     /gene="acpT"
                     /locus_tag="KJL99_000584"
     CDS             45513..46100
                     /gene="acpT"
                     /locus_tag="KJL99_000584"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HBD4435442.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRTLLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRVNWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            46211..47785
                     /gene="nikA"
                     /locus_tag="KJL99_000585"
     CDS             46211..47785
                     /gene="nikA"
                     /locus_tag="KJL99_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HBD4435443.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALNKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            47785..48729
                     /gene="nikB"
                     /locus_tag="KJL99_000586"
     CDS             47785..48729
                     /gene="nikB"
                     /locus_tag="KJL99_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HBD4435444.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGSWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            48726..49559
                     /gene="nikC"
                     /locus_tag="KJL99_000587"
     CDS             48726..49559
                     /gene="nikC"
                     /locus_tag="KJL99_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HBD4435445.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWQPYDPQAIDLPSC
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGH
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            49559..50323
                     /gene="nikD"
                     /locus_tag="KJL99_000588"
     CDS             49559..50323
                     /gene="nikD"
                     /locus_tag="KJL99_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HBD4435446.1"
                     /translation="MPQQIELRNITLQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPYALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            50320..51126
                     /gene="nikE"
                     /locus_tag="KJL99_000589"
     CDS             50320..51126
                     /gene="nikE"
                     /locus_tag="KJL99_000589"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HBD4435447.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWHGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQLV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIAETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            51132..51533
                     /gene="nikR"
                     /locus_tag="KJL99_000590"
     CDS             51132..51533
                     /gene="nikR"
                     /locus_tag="KJL99_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HBD4435448.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(51653..52012)
                     /locus_tag="KJL99_000591"
     CDS             complement(51653..52012)
                     /locus_tag="KJL99_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HBD4435449.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(52009..52284)
                     /locus_tag="KJL99_000592"
     CDS             complement(52009..52284)
                     /locus_tag="KJL99_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HBD4435450.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVCDWLLSIGVKP"
     gene            complement(52343..53467)
                     /locus_tag="KJL99_000593"
     CDS             complement(52343..53467)
                     /locus_tag="KJL99_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBD4435451.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(53467..56202)
                     /gene="rbbA"
                     /locus_tag="KJL99_000594"
     CDS             complement(53467..56202)
                     /gene="rbbA"
                     /locus_tag="KJL99_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HBD4435452.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREARINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(56199..57266)
                     /locus_tag="KJL99_000595"
     CDS             complement(56199..57266)
                     /locus_tag="KJL99_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBD4435453.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(57632..59254)
                     /locus_tag="KJL99_000596"
     CDS             complement(57632..59254)
                     /locus_tag="KJL99_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HBD4435454.1"
                     /translation="MKIGTVAGTNGSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNYGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWE
                     ISQYNVFDIKFETIIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDNVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSIDEMTNALDPVELAEIINKKHHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            complement(59516..>59734)
                     /locus_tag="KJL99_000597"
     CDS             complement(59516..>59734)
                     /locus_tag="KJL99_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HBD4435455.1"
                     /translation="NNTTAFRPKPEDIFKCGQTLKKTKQKFGHYGLGVDQHQKIDNYT
                     MGLNSWKIAPNERGDKKGVPGLSCFQPQ"
BASE COUNT        14111 a        16471 c        15130 g        14022 t
ORIGIN      
        1 tcaagagcct gtccaatgtg cataaaaaaa tccggaaaca agtgagcgtt tccggattct
       61 tacacagcca ctggatcggt caactgatcc ttaactgatc ggcattagac cctttggccc
      121 cgttctattt attagtgaaa ttacaatctc accggatcga tatgccagat atgatcagcg
      181 tactctttga tagtacggtc agaagagaag tagcccatat tggcaatgtt cagcatcgct
      241 tttgcggtcc actcttcctg aagctcgtag agttcgtcga ctttatcctg acaatcgaca
      301 tagctgcgat aatccgccag tacctggtag tgatcgccga agttgatcag cgaatcaacc
      361 agatcgcgat agcgacccgg atcttccgga ctgaatacac cgctgccgat ttgcgtcagc
      421 acctgatgca gctcctcatc tttctcgtag tattcacgcg gtttgtagcc ctgacgacgc
      481 agttcttcca cttcttccgc tgtgttacca aaaataaaga tattgtcagc accgacatga
      541 tccagcatct cgacattcgc accgtccaac gtaccgatag tcagcgcacc gttaagcgca
      601 aacttcatgt tactggtgcc ggaagcttcc gtccctgcca gcgaaatctg ttcagacaga
      661 tctgccgccg gaatgatcaa ctgcgccagg ctaacgctgt agttcgggat gaacacgact
      721 ttcagcttat cgccaatctg cggatcgttg ttgatcactt tcgctacgtc attgatcaaa
      781 tgaataatgt gcttcgccat gtaataggcc gaagccgcct taccgccaaa aatattcacg
      841 cgcggtaccc acttcgcatc cgggtcggcc ttgatgcggt tatagcgggt aatcacatgc
      901 aacacattca tcaattgacg tttgtattcg tgaatacgtt tgatttgtac atcgaacaac
      961 gcctttggat tcaccaccac attcagctgc tgggcgatat actctgccag acgctttttg
     1021 ttctccagct tcgcctgatg cacagcgtga ttaaccattg ggaaatcaca gtgttgttgc
     1081 agctcattaa gcaggctaag gtcggtgcgc cagttacggc ccaggtgttc gtccagcacg
     1141 gctgaaagcg atgggttcgc taccgccagc cagcgacgcg gcgtcacacc gttggtgacg
     1201 ttggtgaaac gacccgggaa gattttcgca aagtcggcaa acaacgattg caccatcaga
     1261 ttagagtgca gttccgatac accgttaact ttgtggctca caacaaccgc cagccaggcc
     1321 atacgcacac gacgaccgtt ggattcatca atgatcgacg cccgtcccag cagatcggta
     1381 tcgttcggat actgttcctg caaggttttc aggaaatagt cgttgatttc aaagatgatc
     1441 tgcaggtgac gcggcagaat tttacccagc atatcaaccg gccaggtttc cagcgcctcg
     1501 ctcatcagcg tgtggttagt gtaggagaag acctgacaac acacctcaaa cgcgtcgtcc
     1561 cagctaaatt ggtgctcatc gatcagcaga cgcatcatct caggaatcga cagtaccgga
     1621 tgggtatcat tgagatgaat cgcgatttta tccgccaggt tatcgtaggt tttatgcaac
     1681 tgataatgac ggcttaaaat gtcctgaatg gtcgaggaaa ccaagaagta ttcctgacgc
     1741 aggcgcagct cacgcccgga gtaggtggag tcatccggat acagtacgcg agatacgttc
     1801 tcggagtggt ttttatcttc cactgccgcg aagtagtcac cctggttgaa tttaccgagg
     1861 ttaatttcgc tactggcttg cgcactccac aaacgcagcg tgttggtcgc gtcggtgtcg
     1921 taaccaggga ttatctgatc gtaagcgact cccagaatct cttcggtttc aatccagcgc
     1981 gtttttttac cttcctgctg aatgcgacca ccaaaacgga ctttatagcg cgtgttgtgg
     2041 cgtttgaatt cccacgggtt accgtattcc agccagtagt ctggcgactc tttctggcta
     2101 ccgttaacga tgttctgctt gaacataccg tagtcatagc ggatgccgta accgcgcccc
     2161 ggcaacccta acgtcgccag agaatcaagg aagcaagccg ccagacgtcc caggccaccg
     2221 ttaccgaggc ctgggtcatt ttcttcatca atcagctctt cgagatttaa ccccatcgct
     2281 tccagtgcgc cctgtacatc ttcgtaaatt cctagcgaca acatggcgtt ggagagcgta
     2341 cggccaatca aaaactccat cgacaggtag taaacctgac gagtttcttg cgacaactgg
     2401 gcacggtttg aacgtaacca gcgctccacg agacgatcgc gcacagcaaa taacgttgcg
     2461 ttcagccatt catgtttatt ggcgacgacc gggtcctttc caatcgtaaa catcagcttg
     2521 taagcgatag agtgcttaag agcttctacg ctaagcgtgg gcgatgaata tgtaaacgga
     2581 gcattcatat aggcgtttcc tgaaaactat ttcaagcgat agtaaagctc acggtacgac
     2641 ttcgccgcga cctgccagct aaaatccatt gccatagcct gacgttgcac aaaccgccac
     2701 agtgaaggac gggaccacag tacaaaagca cgccgaatag cccgtaacag cgaccaggca
     2761 ttactatctt caaagacaaa cccactggcg acgccatctg caaggttctc aagagaacag
     2821 tcagaaaccg tatcagcaag cccaccggtg cgccgcacta acggcagcgt accgtacttc
     2881 aatccataaa gttgcgttaa gccgcacggt tcaaaacggc tgggcaccag aatgacgtcc
     2941 gcgccgccca taatgcgatg cgaaaatgct tcgtgatagc caatctgaac gcccacctga
     3001 ccggggtatt ccgctgccgc cgcaaggaaa ccttcctgca gcaccggatc gcccgcgccg
     3061 agtagcgcca gctgcccgcc ctgctccaga agccccggta aggcttccag caccaggtcg
     3121 agacctttct ggctggtcag acggctcacc actgcaaaaa gcggcacttt atcgtcaacc
     3181 ttaagcccca ttgcgatttg taactggctc ttattttccg ctttatcttc caacgtatcg
     3241 cgggtgtaac gcgaggccaa cagtaagtcc gtctctggac tccagatttt ctcgtccacg
     3301 ccgttcagta cgcctgaaag acgcccttca cggtgacgct gttgcaacag accttccata
     3361 ccgtaggcaa actgcggttc ggtgatctcg cgagcgtagg ttggactgac cgccgtaatg
     3421 tgatcggcat agtacagacc ggccttcagg aaagagattt gtccgttgaa ttccagccca
     3481 tgaatattaa agaatgacca tggcaattgg atgtcattca tgtgatgtgc ataaaacatg
     3541 ccttgatagg ccaggttgtg cacagtaaac accgacttcg caggacgccc gcgcgccgcc
     3601 agatacgcag gcgcaagacc tgcatgccag tcgtgcacat gcaccacatc aggacgccag
     3661 aatgggtcaa gcccgctggc catttctgcc ccaacccacc ccagcagcgc aaaacgcaat
     3721 acgttgtcgg tataggcaaa taagttggta tcgtgatacg ggcttcccgg acgatcatag
     3781 agatgcggcg cgtcaatcag gtatatgcca accccgttgt aatgaccgaa caacagcgtg
     3841 atatgcccgg cgaaagtatc acgacgggat actacctgcg catcggtcac gccacggcga
     3901 atatcgggaa atgcaggcaa cagtacgcga gcgtcaacgc cgtctgcgat ttgtgctgcg
     3961 ggtaatgccc caataacatc agccagaccg ccggttttaa gcagcgggaa catctctgaa
     4021 catacatgta aaacctgcat tatcgctcct gtttatgccc taacttccgt agcatttcgc
     4081 gcgttaccag cacgatgcct tcttctgaac gatagaaacg acgtgcatct tcctctgcgt
     4141 tttcaccaat caccatgcct tccgggataa cacaagcacg atcgatgacg cagcggcgca
     4201 gacggcacga gcgacctacc catacttccg gtaacaatac ggcggaatca atgttgcaga
     4261 atgaattcac gcgaacgcgc gagaacagaa cggactgcac caccaccgaa ccggagatca
     4321 cacaaccgcc ggataccagt gagttaaggg tcatcccgtg gctaccggag cgatcctgca
     4381 cgaatttcgc tggcggtaat gattcattgt aggtgcgaat tggccaattg cgatcgtaca
     4441 tatccagttc cggcaccaca gaggccagat cgagattcgc tttccagtaa gcttccagcg
     4501 tacccacatc gcgccagtac ggctcggcat ccgggtcgga ttgtacgcaa gagagcggga
     4561 acgggtgcgc ataggccaga ccggcttcgg tgatcttggg aatcaaatct ttgccaaagt
     4621 cgtggctgga gttctcatca cgatcgtctt cttccagcag ttcatacaga tagtcggcgt
     4681 caaagacgta gatacccata ctcgccagag atttgctcgg atcgttcggc attgacggtg
     4741 ggttagcagg tttttcaacg aattcgataa ttttatcgtt ctcatcaacc gccataacgc
     4801 caaatgcgga ggcttcttca atcggtactg gcatacaagc aacggtgcaa cgtgcgcctt
     4861 tttcgacgtg atcgataagc atacgcgagt agtcttgctt gtagatatgg tcgcccgcca
     4921 ggatcaccac gtattccgct ttataacggc ggataatgtc gaggttttgg gtgaccgcat
     4981 ctgcggtgcc gcgataccag ttttcccctt tcattctctg ctgtgctggc agcagatcga
     5041 caaactcgtt catttcttca ttgaagaatg accagccgcg ctgaatgtgc tgcaccagag
     5101 tgtgggactg gtactgggtg atcacgccca tacgacggat cccagagttg atgcagttag
     5161 acagcgcaaa gtcaataatg cggaacttac cgccgaagtg tacggccggt tttgctcgct
     5221 tattggttaa atccttcagg cgggtaccac gtcctcccgc cagtatcagg gcaacagatt
     5281 tcaatggcag ctggcgcgcc aacattaagt gatcgttctt ctctaaacta accatgacta
     5341 actccttttt tatcatctct ggaacacaca caatccgtgt gcaggtccct gccagacaac
     5401 cgtaatcact gggttatcct ctccagcgaa tgggggaatg gcgtgccact ccccagcagg
     5461 taaaacaatc tctgttacct caagcgtggc gttaattgcg atcaaaaagc gatccgagag
     5521 cagaatttgc agctgtttcg gcccgttttg ccactcatcc gtgcttaaag gttgagcata
     5581 tcgatttagc caacggacat tgccgtcacc ttcttcccac cagcgattct ccaccaaagc
     5641 gggaatgcgc ttgcgcagat ggattaacgc ggcggtaaat gcggttaaac cactgcttgc
     5701 ctgcgaccag tccaaccagt ttaattggtt atcctgacag taggcattgt tattgccatg
     5761 ctggctgtga ccatgttcgt caccggccag taacatcggc gtgccctggg agagcaacaa
     5821 cgttgttaac agggcatgaa tgctgtcgcg ccgccgttca accaggtcaa gagtaccgcc
     5881 taacccttct ttaccatgat tgttactgta attgttgttg gtcccgtcgc gattttcttc
     5941 tccgtttgct tcattgtgtt tatggttgaa gcaaacgcag tcgcgaagcg taaaaccgtc
     6001 atgcgcggtg acgagattaa tcgcggcact cggcagacga ccattacgtt taaaaacatc
     6061 gctggaggca gcaaaacgcc cggcaaacgc ccccagaggc aaatcataat gtagccagaa
     6121 acgacgggca gcatcgcgga aatgatcgtt ccactcggca aacagcggcg ggaaatttcc
     6181 cacctgataa ccactaggag cgatatccca cggttcagca attaacttca cctgcgagag
     6241 caccgggcag ttctggatag cggtaaacaa cggcgcatcc tgacggaact ctggcgtacg
     6301 gcccatgact gccgccagat caaagcggaa accatcgacg tggcaggttt ctacccaata
     6361 acgcaggcag gcgctggcat aatccaccac cgccggatga ctcaaattga gcgtgttgcc
     6421 gcaaccggtc cagttgtgat aatcgccgtc ttctcttatc caataatagc tacggttatc
     6481 gatcccacgc agcgagaata acgggccgtc gaggtccagt tccgcactat ggttgagcac
     6541 gatatcaaga atgacttcga tacccgcttt atgcagtgct ttgattgcat cgcgaaactc
     6601 atccagcgcc gtttctggcg agcaggcata cgccggatgc agcgcaaaca tcgccaccgg
     6661 gttgtaaccc cagtagttac ttagccccat gcgttgcaga cgtggttcac tggcaaactg
     6721 cgccactggc agcagttcca gcgcggtaat gcccaattgt ttcaaatagt tgatcatcac
     6781 cggatgcccg agggctttat aagtgccacg gatctcgacc gggatctccg ggtgcaagta
     6841 cgttaatcct ttgacatggg cttcataaat gatggtgctg ccccacggcg tgcgcggcgg
     6901 ggcgtcatct tcccagtcat agtgatcaac caccactacg catttcggcg caatggcggc
     6961 gttgtcgcga tagtcaggtt cattatgacc ggcgtgcagc agcgggttat ctttaaactc
     7021 cccgtcaatt tgccgcgcgc aaggatcaat caacaacttc gccgggttaa agcgatgccc
     7081 ctcggcgggt tgccaggggc catgaacgcg ataaccataa cgcaaacccg ggcgcgcatc
     7141 cggcagataa ccgtgccaaa tgtcgccact gttccctggc aagtcatagc gatgttcctg
     7201 gccattggcg tcaaagacac acagttctac ccgctcggca tgagcggaga aaagtgtgaa
     7261 gttgacgccc tgaccgtcgt aatgcgcgcc gaggggagcg ggtttgccaa tggcgagttg
     7321 tgtcattctg cctcccgaac cagccagata gtggccagcg gtggtagcgt caggcttagt
     7381 gaatgctgac gaccgtggct ggcaatctca tcgctgtgta ccgtgccgcc attgcctgca
     7441 ttactgccgt gatagtgtat ggaatcggta ttgaggattt cacgccattt acctggctga
     7501 ttaatgccga agcgataatc atgacgcggc accggcgtaa agttactggc aacgataatt
     7561 tcgttaccct ctttatcgcg acgcacaaag atcagcaccg agcgttcttt gtcatccacc
     7621 accagccatt caaagccgta cgggtcaaaa tccagttcat gcattgcttt atggtggcgg
     7681 taggtgaggt tcagatcgcg caccagacgc tggacaccgt ggtgccagtt atcgccgcct
     7741 tccaacagat gccagtcgag gctggcgtca tggttccact cgcggccctg ggcaaattcg
     7801 ttccccatga acagcagttt cttgcccgga aatgcccaca tccagccata gtaagcgcgc
     7861 aggttagcga atttctgcca tgcgtcgccc ggcatgcggt cgagaatcga ttttttaccg
     7921 tggaccactt catcatgcga caacggcagg acgaagtttt cagtgtagtt gtagagcatc
     7981 ccgaaggtca gtttatcgtg atgatactga cgataaatcg ggtcgagctt catgtagtcc
     8041 agggtgtcat gcatccagcc gaggttccac ttgtaccaga agcccagacc gcccatatcc
     8101 tgcggacgag aaacgccagg gaaatcggta gactcctcag ccattgtcac cgcaccggaa
     8161 acctgctcac caagaatacg gttggtatta cgcaagaatt caatcgcttc aagattctcg
     8221 cgcccgccaa attcgttcgg gatccactcc ccctctttac ggctgtagtc acgataaatc
     8281 atcgacgcca ccgcatcgac gcgcagcgca tcaataccaa aacgttcaat ccagtaaagc
     8341 gcgttaccga cgaggaagtt actgacttca cggcgaccat agttgtagat cagcgtgttc
     8401 cagtcctgat gatagccttc acgcggatcg ctgtgttcat acaagttcgt gccatcaaat
     8461 tcggcaagcg caaagtcatc agtcgggaag tggcctggca cccagtcgag aatcacgttc
     8521 agaccagctg cgtgtgcggc atcaatgaaa taacggaagt cgtcgcgagt accaaaacgg
     8581 cgggttggtg catacaggcc ggttggctga taaccccaac tgccatcgaa gggatgctcg
     8641 ttaatgggca gtagttcgag gtgggtaaag cccatccatt tagcataagg caccagttga
     8701 tcggccagct cgcggtagct caaccagaaa ttgttgtcgg tgtggcgacg ccaggaaccc
     8761 aggtgaactt cataaataga gattggcgca tcaaactgat tcgctttttt gcgctcttca
     8821 gtctgtacaa ccttttccgg cagcccgcaa ataagagacg cggtttccgg gcgcatttgc
     8881 gcttcgaagg cataagggtc ggacttcaga cgcaagttgc cattggcatc aatcatctcg
     8941 tatttataga gctgaccgtt atgcgcccca gggataaaca gttcccagat gccgctctct
     9001 ttacgcaggc gcatcgggtg acggcgaccg tcccagtagt tgaattgccc aaccaccgag
     9061 acccgacggg cgtttggagc ccagacagag aaacgcgtac ctgtgacgcc atccatagta
     9121 tctgcatgcg cgcctaaggt ttcatacggg cgcaggtgag taccttcaga taatagccag
     9181 gcatccattt cctggattag cggaccaaaa cggtaaggat catcaatcag gttttgctga
     9241 ccatgccaga caacagccaa ctgatagcgg aaaaaattct tacgtcgcgg aatgacgccg
     9301 ctaaagaatc cccgtgagtc gagacactcc agttttgcga gtttgcgccc ggttttcggt
     9361 tcaatcaccc acacatcggt agcgtcgggt aaaagggcac ggacttccag tcccgcggtg
     9421 gttttatgca ttcccagtac ggaaaaagga tccgcaaaat ggcctgcaat tagcgcgtta
     9481 atcacgtctc tatcgatacg atcggacatg cttgtcttcc tgttttattg tgtcacccca
     9541 tccaattgga tttttgactt ctggttgtga catttttttg acctgaacgg cgcagcactc
     9601 tgtgcatcct ctctgcgtcg tcctcacttc aggtaaggct gtgaatactc atgtattcag
     9661 ccacccttaa agaatagcca atgctctatt taactcccgg taaatcatga aacatctgcg
     9721 cttactcctg tattacgcac taacaggggc ggcatcgcgc cccagattta atgaataaag
     9781 attacgccag ttgacgaagc atccgacgca gcggctccgc ggccccccac agcagctggt
     9841 cgcccacggt aaaggctgac aggaactctg gtcccatatt cagcttacgc aggcggccta
     9901 ccggcgtggt cagcgtgccg gtaacggcag ctggggttag ctcacgcata gtgatttccc
     9961 gatcgttcgg aacgactttc gcccacggat tgtgcgcagc cagcagttct tcgacggtcg
    10021 gaatagacac atcttttttc aatttaatag tgaatgcctg gctgtggcag cgcaatgccc
    10081 cgacacgcac acataaacca tctaccggaa ttacggaaga tgtgttgagg atcttgttgg
    10141 tttccgcctg ccctttccac tcttcgcggc tctgaccgtt atcgagctgt ttgtcgatcc
    10201 acggaatcag gctacccgcc agcggcacgc caaagttatc caccggcagc tcaccgctac
    10261 gggttaaggt tgtgactttg cgttcgatat cgagaatagc agaggacggg gtcgcgagtt
    10321 catctgccac atggccatac agatggccca tctgggttaa taactcacgc atatgtcgcg
    10381 caccaccgcc ggaagcggcc tggtaggttg caacggacac ccaatcaaca agatcattgg
    10441 cgaataaacc acccaacgac atcaacatca ggcttacggt acagttaccg ccaacaaaag
    10501 tcctgatgcc attatttaat ccgtcggtaa tgacgtcctg attgacgggg tcaagaatga
    10561 tgatggcgtc atctttcatg cgcagagacg atgctgcgtc aatccagtaa ccttgccatc
    10621 cgctttcacg aagctttgga tagatttcgt tggtataatc gccgccctga caggtcacaa
    10681 tgatatcgag ggcctttagc gcctctagat caaaggcatc ctgaagtgtg ccagtggttc
    10741 cgccaaaaga cggcgcagcc tggccaagct gagaagtaga aaagaagaca gggcgaatgg
    10801 cgtcgaagtc gcgctcttca accatgcgtt gcatgagaac ggagccgacc ataccgcgcc
    10861 agccgataaa accaacattt ttcataagcg ttttttcctg caaagatgtg tgctgtataa
    10921 atgtgccggt ctcctcttgg cacatctttc accatacaaa aagctgccaa agtcgcaagt
    10981 gaaattaatc aatgatagcg aagccatcag taatgcgact tatcctgctt tgttagcacg
    11041 cagaaagtcc gcggcaatta tcagggaatt tgagttatga atgaaatcat ttctgcagca
    11101 gttttattga tcctgattat ggatccgctc ggaaacctgc ctattttcat gtccgtactg
    11161 aaacatactg aaccgaaaag acggcgggca atcatggtgc gagagttgct tattgctctc
    11221 ctggtgatgc tggtgttcct gtttgcgggt gagaaaattc tggcatttct cagcctgcga
    11281 gcagaaaccg tctccatttc tggcggcatc attctgtttc taatcgccat taagatgatt
    11341 ttccccagcg cttcaggaaa tagcagcggg cttccggcag gtgaagagcc atttatcgtg
    11401 ccgttggcaa ttccgttagt cgccgggccg actattctcg ccacgctgat gttgttgtct
    11461 catcagtacc cgaatcagat gggacatctg gtgattgctc tgctgctggc ctggggcggc
    11521 acctttgtca tcctgctaca gtcttcgcta tttttacgtc tgctgggcga gaaaggggtg
    11581 aacgcacttg aacgcctgat gggattgatt ctggtgatga tggcaactca gatgttcctc
    11641 gacggcattc gaatgtggat gaaggggtaa cgaggagagt ttttcgaaaa gagcccggta
    11701 gcgtcacaac taccgggcga acaaacttaa ctcaacagct ggaacgcaat catcccaacg
    11761 atggcaccaa cagtgccgag gatggtttcc atcatggtcc aggttttcag cgtttcggct
    11821 tcggtcgcgc cggtaaattt accgaacaac cagaaaccag cgtcgttaac gtggctgaca
    11881 acaatcgaac caccagcgat acaaatcgac agtgccgcca tttgcgcacc agagtagttc
    11941 agttgttcaa tgactggcat caccagtccc accgccgtta aacaggctac ggtggcagcc
    12001 ccctgaatga tgcgcactgc agcagccagc acgaagcagg tgatagcaat cggcaggccc
    12061 atgccggtta acgcttcgcc cagtgccgga cctacgccag agtcaaccag cacctgtttg
    12121 aacacgccgc ctgcaccaat caccagcaga atgatccccg ccggttgcag cgcgtgaccg
    12181 caaatctcca tcactttgtc ttttggcatg ccctgacgca ttgccaggcc ataaatcgct
    12241 accagacaag caaccagaat cgcggtaaac ggatgaccaa taaactcgaa ccattcgtaa
    12301 gcggtagagc cttctggcac aaaacgcgcg gcaatggttt tcagccccac cagcaccagc
    12361 ggcaacagga tcagcgacag gctgaatccg aaagatggca ttttaccttc gccgagatgc
    12421 ggttcgctga tgtcgtcagg aatatgcagc tcaacgtaac ggctgatgaa gttaccccac
    12481 agcggcccgg caataatcat tcccggaatt gccgcacaca ggccaatcag gatcatccag
    12541 ccaaagtcgg cattcatctg cgatgccagc agcattggcg ctggcccagg caccaggaac
    12601 gctgccgcgg ccgccacgcc tgcgaataat gggattacca gcttcaccag gttcgtaccg
    12661 gtgtggcgcg ccattgagaa agcaacgcta atcagcagaa caatcgccac ttcaaagaac
    12721 agcggtagcg cacagaccag ccccgcaagg ccgatggcat aatgcgcgcg gctgtgaccg
    12781 aaggatttga gcattttgac ggcaatctga tcgactgcgc cggtttcata taagatcttg
    12841 ccaaacatag ctcccagggc gacaaccacc gccaggaagc cgagggtgcc tcccatccct
    12901 ttttccatcg tcgctgcgat tttatcgagc ggcataccgg aaaaaaggcc agcccccatg
    12961 gacaccacca tcaaagccag gaaagcgtgc atacgcgcct tcatgactaa aaacagcagc
    13021 agtaaaacag accctactgc tgttaaaaca agcgttaatg tagtcactac ttatttgcct
    13081 tttttaataa cctcaatggt gcttgccaca acaccttcca gcggttgatc gatatccacc
    13141 accagtacat cggtttcgtc cgcacccggc tcctgcagcg tttcaaactg cgtcaccaac
    13201 atttgggttt taaagaaatg gcctttgcgc gctttcaggc gactttcaat cacatcaaaa
    13261 tcgcctttca ggtagatgaa agagagattc ggattacctt cacgcagcaa gtcgcgatag
    13321 tgctttttca gtgcagaaca ggcgatcagc gacactttat tggtgcgctg catagcaaac
    13381 gcggagtcgt tcagcgcctg caaccagggt ttgcgatcgt cgtcattcag cggttcgcca
    13441 gacgccattt tttcgatatt gcgccgtgga tggaggaaat cgccatcaag aaacgcggca
    13501 tgaagttgat gcgccacttc actggcgacc gcagatttgc cgctgcccga tacgcccatc
    13561 aagacgtaaa tgtggtgatc atggttagtc gtgctcaaag tggtgccccc acaatacaag
    13621 aattaaaaat gttacgggta actgttatcg gcaacattgt ccagccggac aatatcagaa
    13681 gcaatatcca tccgtgcctt aagtgtataa gtgtgagcta cttcaaattt gtgggcttaa
    13741 atagatccgc ccggtgacaa ggtgaaacct aaatctaaca ttttcggtgt cacagattcg
    13801 ccacgaatac gcgccagcag gcgttcagcg ccaatactgc ccatccgctc acgcggcgtc
    13861 agcacgctcg caagtcgtgg ctccatcacc tgaccaatgt catgaccgtg gaaaccggca
    13921 atcgccatat cgtcaggaac ttttaacccc agacgctgac attcaaacgc cgcgccgacc
    13981 gccagatcat cattagtaca gaacacgcca tccagctgcg gatattcccg ccgcgcctgg
    14041 cgaatcagtt caataccgga agagtaagaa gaagattgct caaccatcac gctatatggc
    14101 accaggcctg catccagcat cgcctgttcg tatcccttct gtttgatgat agtacgttcg
    14161 tcgagacgtg cgccgagata ggcaatgtgg cgatgcccac gagcaataat ggcggtagtc
    14221 atctggcgtg ccgcttcaaa gttatcaaaa ccgacggcga tatcgaggca cggcgactgg
    14281 ctgtccatca actccaccac cgggatcccc gccacttcaa tcatctttaa ggtgcgcggc
    14341 gtgtgggtac gttcggtgag gatcaggccg tcgatattcc aggagagcat ggattcgaga
    14401 cgttcttgct ccatttccgg tttataaccg tagtgcgcca gcatggtctg ataaccgtgc
    14461 gcgtcggtga cgctttcgat tccgcgtaat acttccgcga aaacctggtt ggtgagagaa
    14521 ggtaacagga cgccaatcgc ccggctggta gcgttagaga ggatatcggg cgcacgattg
    14581 ggaatatagc ccagttcatc aagagcggcg gcaatcttgc cgcgtagagc gacggaaacc
    14641 tgctccgggt tgcgtaaaaa acggctgacc gtcattttgg tcacgcctac acggtcagcc
    14701 acatcctgaa gtacgggtct tttctttttc atcgtcctga aggtacaaaa gagatagatt
    14761 ctcttagttt aacacggacg catcagaacc ttcccgggga aaacagtgct tgtttacaat
    14821 tattagatat acactaaatc aattgcatcg cattgtgcta acgacgcggc aaaaccccgg
    14881 aagcgtgcat cagcacgcga ccggagcgag cgaggaagcc tgcaaaaagg caggcttcaa
    14941 agatgccgtt gagttttaaa ccggcggcag atcgaacagt aacacttcgc tgtcgctatc
    15001 cgcatggatg gagattgcct gctcatccca gattgccaga ccatcgctgg tcgaggcttt
    15061 cacgccgtta atggtaacat tgccttttac cacctgaatc cagacgcggc gttcagcggc
    15121 aatctgatgc accgactgct catctttcag caacgcccaa cgatacagtt ccatatcctg
    15181 atgcactttc agcgaaccat ctcgcgcatc cggcgagagc accagctgtt tgccctgcac
    15241 ggcatcgaag cgacgctgtt cataacgcgg cgtaatgcca ttttcttcag gcatgatcca
    15301 gatctgatac agatgcagac gctcggtgct gcttgggttg tactctgagt gacgaatacc
    15361 cgtacccgca ctcataatct ggaactcacc cgccggaacc tgctctttat tgcccatgct
    15421 gtcctgatgc tcaacagtac cttccagcac gtaggtcaaa atttccatat ctttatgcgg
    15481 gtgagtgccg aagccctgcc ctacttcaat cacgtcgtcg ttaatcacgc gcagcgcgga
    15541 gaagcccata aagttcggat cgtaatagtt ggcacaagag aaagtatgcc aggagtccag
    15601 ccagccatga tttgcatgac cacgttcatt tgctttgcgt aagtagatca tttgtattcg
    15661 ccccctgaat gatttcgatg ggcttagtgt ggacccgatc tgcctgggat gatagagggt
    15721 gaaaattgcc ccctctgttc aaaaaaattg aactattcga ggggccatca aacttaattc
    15781 gcgagagtca ctgtggaggg agaggcttgc tcaaatccgc gttcaaggat ttccagattg
    15841 gtaagaactt cagattcctt gacgtaattt ggcgcaccgt gggtgatggt ttgatacaac
    15901 gcatcataaa cacgcccgta atcgcccatc tccggcttca tctcttctct gaccgtcaca
    15961 ccctcgtcat tgacatactc cagcacaccg accgaatcat ccgctgcgaa tcccggttcg
    16021 cccggcataa tattagcctt caggctggtt tcctgctggt cgataccgta tttaataaac
    16081 gaacctttct taccgtgaac gataaatttc ggataatcga ttttcaccag atggctggtt
    16141 ttgacgatgg ctttcaggtc gccataaaac agttgcgctt caaaggtgtc gtcaggattg
    16201 gctttattac gcaggctgcg gatgtcataa gcgacgtgat ccgggcgacc gaacagagaa
    16261 ataatctggt ccatcgtatg cacaccaagg ccatagaacg cgccatcctg cggcagtcca
    16321 ggtttggttt ctgccaccgg gcggtaatag tcaaaatggc tttccacttc gacaatctct
    16381 cccagcttgc cgctttcaat cgcttttttc gccgtcagga agcaggaatc aaagcgacga
    16441 ttctgatatg gcgtgacggt cagccctttg cttttcgcca gtgcaaacag ctctttcgcc
    16501 tgcgcaagtg tcggagtgaa cgttttttcg accagcacat ttttcccggc ttccagcgcg
    16561 cgcttcgcgt actcgaagtg gctgtccgca tgggtgcaga caacaaccaa cttaacatcg
    16621 ggatcgttta gcacttcgtc gagatcgctg gtgaaatgga tatgagagta aatgggagcc
    16681 tgttcttccg gcttcgcatg gcgacgaaaa atatgcgcga catgccagct atccttgcgg
    16741 ttaagtacat acggcagatg gtaacgggtg gtgcttttgc cgaagccaat aaaggcgcag
    16801 ttgatgacca tgatttcgtc ctttttaagg tggttattta caccttagcg caaagcggac
    16861 gtggttccta ctggagtgcg cataacgaac acaagcactc ccgtggataa attgagaacg
    16921 aaagatcaaa aaaaaagcca gcacccggct ggctaagtaa tactggaagc aatgtgagca
    16981 atgtcgtgct ttcaggttct ccgcgagggt cttcctgatc gcgatacaat aataatcatt
    17041 ctcattcgca cttgtccaac actttttgca aaaaaatgca tttgactcgc atttgaaagt
    17101 caatgatgtt gaaagggaca tttaccccaa agaggacaaa ggaatgagtg agatagtaat
    17161 acgccacgca gaaacacggg attacgaggc catcaggcag attcacgccc agccggaggt
    17221 gtattgcaac acactacagg tgcctcatcc ttccgatcat atgtggcagg agcgactcgc
    17281 cgatcgtccc ggcatcaagc aactcgtcgc ctgtattgat ggagacgtcg tgggccatct
    17341 caccattgac gtgcaacagc gcccacgccg cagtcatgtt gccgattttg gtatctgtgt
    17401 cgactctcgt tggaagaacc gcggcgtcgc cagcgccctg atgcgagaga tgattgaaat
    17461 gtgcgacaac tggttacggg tagatcgcat tgaactaacc gtgtttgtcg ataacgcgcc
    17521 agcaattaag gtctataaaa aattcggctt tgagattgaa gggaccggta agaagtacgc
    17581 attgcgtaat ggtgaatatg tcgatgcgta ttatatggcg cgggtgaaat agagaatttg
    17641 caatctaagt ttgtcattta agccctcacc taccgggagg gcttaagata ttaatacccc
    17701 gccgttaaat catccaccga gcgcgggtcg gatgcgccgt acaactcacc gtccggccca
    17761 accataatgc tttgtgtgct gcccatcgcc tctttcagcg ccactttctg accttttgct
    17821 tccagcagct tgagcgtatc cgggctaaac cctttttcga cacgcagctc gtccggcaac
    17881 cactgatggt ggaaacgcgg cgcattggtc gcttcggcga cgttcatgcc ataatcgatg
    17941 ctattcacca ccatttgcag caccgtggtg ataatccggc taccgcctgg gctaccggta
    18001 accagccagg ttttaccgtc tttcaccaca atggtcggcg acatcgacga cagcgggcgt
    18061 ttgttcggcc cgacggcgtt ggcatcaccg cccaccagcc cgtaaacgtt cggtacgccc
    18121 ggtttggcgg agaaatcatc catctggtta ttaagcagaa taccgctctc acccgcgaca
    18181 atacccgtac cgaaggtggt gttcagcgta taggtcaccg ccaccgcgtt accgtcttta
    18241 tccactacag agtaatgggt agtttgatta ctctcataag gcgcaagctt gccagggcga
    18301 atctcactgg acggcttcgc ttttttgata tcaatttgat cggcaatgga tttggcatag
    18361 gctttattgg tcagtgcctg ccacgggact ttgacaaaat ccgggtcgcc aagatattcc
    18421 gagcggtcgg cgtaggcgta tttctccgct tctgccatga tttgcatcgc atcggcgctg
    18481 ccaaagccgt atttcttcat atcgaagttt tccagaatat tgaggatttg tacgatatgg
    18541 atcccgccgg aggatggcgg tggcatggag taaacctgat acccgcgata atcgccgctt
    18601 atcggagtgc gttcgaccgc tttataggcc gctaaatctt ctttagtgat caagccaccg
    18661 tttttctgca tctcctgggc gatctgttcc gcaatcgtgc ctttatagaa ttcgtccggg
    18721 ccgttttcag caatcatctc caggctcttt gccaggttcg cctgcaccag cgtgtcgccc
    18781 tttttcagcg gctcgccctc tttccagaag atagctttac tgttttcgtg attcggcaac
    18841 acttcgctac cgtaggtttt gagatcgtca gccagcgcgt cgttaacgat aaaaccatcg
    18901 cgtgccagtt taaacgcggg ctgcacgact ttgttcagcg gcatggtgcc gtatttatcc
    18961 agcgccagcg agaaacctgc taccgtaccc ggtgtgccgg aagccagatg cgaagtgagt
    19021 gattttttgc tgtccgggtt gccctgatca tcgaggaaca tatcgcgggt cgctttggcg
    19081 ggtgccattt cgcggaaatc gatagccgtg gtattgccat ttttcgagcg gattaacata
    19141 aaaccaccac cgcccagatt ccctgcctgc ggatgcgtta ccgccagcgc gtagcccacc
    19201 gccacggcgg catcaacggc attcccgccc tccttgagga tatccacccc cacctgagtg
    19261 gcagtggcgt ccacagacgc taccattccc tgtttcgcgc gtaccggatg gaagacatct
    19321 tcctccacac catacgagac gggcggcgca ggaggcgcgg cggcggcgct aaaacaactt
    19381 cctgagagca gagcagcaat ggccacccgg cgtaaaaacg tcggttttat catcgttatt
    19441 ctccagagat taaggggcaa ccccagctaa gcctggtata taactctgaa ttaatcatcg
    19501 ttttgtcggg aagcgagtaa acttaaagga tatcttcaga ggaggatacg cgatgaaacg
    19561 acttctgatt cttacggcac tcctgccgtt tgtcggcttt gcacagccca ttaatactct
    19621 gaacaaccct aaccagccgg ggtatcagat acccagccag cagcggatgc aaacccagat
    19681 gcagactcag caaatccagc aaaaagggat gctgaatcag caactgaaaa cgcaaactca
    19741 gttgcaacag cagcatttag aaaaccagat aaacaataat tctcagcggg tgttgcagtc
    19801 acagccgggg gagcgaaatc ccgcccggca gcaaatgctg cccaacacca acggcgggat
    19861 gttaaacagc aaccgtaatc cggatagttc gttgaatcag cagcatatgc tgccggagag
    19921 gagaaacggc gacatgctga atcagcccag cacgccgcag cctgacattc cgttgaaaac
    19981 tattgggccg taaagttcgg accaatcacg tcaatcgcat cggtacagat gcaatccacg
    20041 ccccagcgca gcaactctgc cgcgtgctgg ggtttattga cggtataaac cagaatccgt
    20101 aatccggcgt ctttcaactg catcactcgc gctttatcga gtaacttatg attgagatga
    20161 atagagacgc agcccagccg cgcggtcagt tcgcgccagt cgtcgcgcca ctcatcaagc
    20221 aataaaccac gcggcagttc cggtgccgcc tgttgtgcag cttctaaagc atcaatctca
    20281 aacgatgaca gcagcggcgg cgtcataccg gcccacagtt ggcgtgccgc cagcgccacc
    20341 attttgcccg ttaatggtcc agtgccagtg gtgggtttga tttcgatatt cgccatcatc
    20401 ccgtgttcgc gacagcgttc cgccacctgc gaaagcaacg gcagcggctc acctttaaac
    20461 gctttgctgt accaactgcc cgcatccacg cgcagtaaat cctgccagtt cagttcaccc
    20521 gcgacgcccc agccgttgct ggtgcgttcg agattgtcgt catggagcag gaagatctcg
    20581 ccatctttcg ataacttcgc gtcaaattcg atcatcttat gaccgtattt tgccccgacg
    20641 tcgattgccg ccagggtgtt ttccggggcc agcttaccgc cgccacgatg agcgacgatg
    20701 cggggataag gccagttact catactcgtt gtcctgtttc accatcaaaa agatgcagct
    20761 gattttccgc cagatgcagc cacagcgtgc tgcctgccgt cgggcgctcc tgatgcgcca
    20821 gtcgcaccac cagcttctgc ttgcgagctt agcgcaatat gttccgggcg aatgccgagg
    20881 gtcattttac gcccggcata ctgacggtag ccaccgttta gcggtagcac gataccgccg
    20941 tccagttcga aatgcgtgcc ttcattattc atgcggcctg tcagcaggtt catcgccggg
    21001 ctgccgataa aactcgccac aaacaggctg gcgggctttt cgtagacttc caccggcgtg
    21061 ccaatctgtt cggcaacgcc gccgttcatc accattactc gctgggcgag cgtcatcgcc
    21121 tcaacctgat cgtgagtaac gtagagtgaa gtcgttttca ggcgacggtg cagctgttgc
    21181 agttcaagac gcatctgtac gcgcagcttg gcatcgaggt tagagagtgg ctcatcaaac
    21241 aagaacaccg ccggatcgcg cacaatcgcg cgtcccatcg ccacacgctg gcgctgaccg
    21301 ccggaaagct cgcgcgggcg acgtttgagc agaccgtcca gctccagaat gcgcgccgct
    21361 tctttaacgc gctcggctat ttgctgcttg cccatgccgc gaattttcag cccccacgcc
    21421 atgttttctt cgacgctcat atgcggataa agcgcgtaat tctggaacac catcgcaatc
    21481 ccgcgatctt tcggctccat ttcggtcacg cgctggtcgt tgatccagat atcgccttcg
    21541 gtcaccctct ccagcccggc aaccatacgc agcagcgtcg atttcccgca gccagacggc
    21601 ccgaccatca cgataaattc gccatccgcc acatcaaggg tcagcggttt aatgacctag
    21661 gttttgccat cccagctttt ggttactgcc tgtaatttca gtcctgccat cttatttctc
    21721 actatcgacc aggccgcgca cgaaggcacg ctgcatcact aaaacaatca ctactggtgg
    21781 gatcagggtt aacaacatcg ccgccatcac tgagttccat tccgtggtgc cttcgccagt
    21841 ggcgatcatc cctttgatcc ctgccacggt ggtgccgaga tccacgtcgg taataatcaa
    21901 caacggccac aaatactggt tccagccgta aataaaggtg atcacaaaca gcgccgccag
    21961 attagttttg gagagcggaa aaacgatgtc gcaaaagaag cgcattggtg acgcaccgtc
    22021 gatccgcgcc gcttccacca gttcatccgg cagcgtcata aagaactggc gaaacagaaa
    22081 cgtggcggtc gccgaggcca tcagcggcag cgttaaaccg gcgtagctgt cgagcatctt
    22141 caggttggcg atgacttcca ccgtcgggaa gatacgtact tcaaccggca gcatcagggt
    22201 gataaaaatc atccagaaga agaggttacg tagcggaaaa cgaaaccaga caatggcaaa
    22261 tgccgagagc atcgagacag taattttgcc gagcgtaatg ctgaacgcca tcacaaagct
    22321 gttaagcaac atccgccaga acggcgcgct atttgtgcct accccgttca cccagatgtt
    22381 gtggatgttt tccagcagat gcgtgccggg gatgagcgtc atcggcgcgg cgtagacggc
    22441 ctgtttatcc agcgtcgccg cgacaaacgc cacgtacagc gggaagagga tcaccgcgat
    22501 cccgaggatc agcatggtat ggctgaatat cgtcagccac ggacggttct caatcattgg
    22561 taacgcacct tgctttcaac atagcggaac tgcaccaccg tcagcacgat gacgaggaac
    22621 atcagcacca tcgactgtgc ggcagacgaa gccagatcca gtccggtaaa accttcgcgg
    22681 tagatcttat aaatcagcgt cgtggtggcc tgaaccggcc cgccggacgt ggcggcgtcg
    22741 atcaccggga aggtgtcgaa gaaggcatac accaggttca ctaccagcag gaagaaactc
    22801 accggggcga taagcggcag cgcaatctta aagaagcggc gaatcggccc tgcaccgtcg
    22861 atggctgcgg cttcgatcaa cgaacgggga atggattgca gcgcggcata gaagaacaga
    22921 aagttgtagc tgatttgctt ccacaccgag gcaaacacca ccagaaacat cgcctgaccg
    22981 ctattttgcg cgtggttcca gtcgtagccg aactcagcga gaaaatgggt gatcagcccg
    23041 cgaccggggt taaacaggaa gatccacaat acggcggcaa cggcgggagc cacggcatac
    23101 ggcagcaaca ttaaggtttg gtagaaacga ctgccgcgca cgatgtactc caccagcgcc
    23161 gcaaagaaca gcgataccag caaaccgctg acggtgacaa aggtgctgaa ttttatcgtc
    23221 gtccagaagg agtcgagata atagctgtca tgaaacagcg tgacgaagtt atccagaccg
    23281 acaaactggc tggagaaacc aaacggatcg acgctttgta gcgagtacca caacgcttcg
    23341 cccgcaggcc agataaaaaa gataacggtg atgatgagct gcggcgcgac cagcagataa
    23401 ggcagccagc gcgagcggaa caccggacgg gatgatgaca ttacggttaa ttcctgaact
    23461 gtgccggatg cgcttcgctt atccggccta cacgcgatgc caggtcagat aagcgaaacg
    23521 catccggcat tacactgatt aagacttcgt cgatttctca aagcggcgca gtaactggtt
    23581 cccacgctca actgcggtat ccagcgcctg ctgtggcgtc ttcttaccgg tccacacgct
    23641 ctcaagctct tcatccacaa tcacgcggat ctgcggcatg ttgcccagac gaagcccttt
    23701 ggtgaacggc aacggcggct tgttcagcat ctgacgcgtc gcggtatccg cacctgggtt
    23761 tttctcgtaa aagccctgct cacgggtcag gtcatacgcc gctttggtaa ttggcagata
    23821 accggttttc tgatgccact cagcagcgtt ttctggcttc gcgaggaagt cgagaaactt
    23881 cgccacgccg gtatacgttt ctttatcttt gccctgcatc acccacaggc tggctccgcc
    23941 gataatggcg ttttgtggcg catctttcgc atcggcgtcg taaggcatca tacctacgcc
    24001 gtagttaaat ttggcgtact cgcgaatgtt ggcaagagaa ccggaagagg cggtggtcat
    24061 cgcgcaatca ccgttataga acttctcggt ggattcatcc ttacgaccga cgtagctgaa
    24121 gtcgcccttc ttgttcatct cctcgagcat ggcgatgtgt ttcacctgct ccggcttgtt
    24181 gaactccagc accgcgtccg tgccgtcaaa gccgttgttt ttgctggcaa acggcagacc
    24241 gttccaggcg ctaaagtttt ccagttggat ccagccctgc cagccgctgg cgtagccgca
    24301 cttaataccg gaggctttca gttttgcgga atagtccgcc agatcctgcc aggttttcgg
    24361 cggctgttcc gggtctaacc ccgctttctt gaaggcgtct ttgttgtaat agagaacgga
    24421 agtcgagctg ttgaacggct gggagagtaa gtggccggtt ttgctgtcgg agtagtaacc
    24481 ggaaaccgtc ggcacaaact gcgactcatc gaactgaatc cctgcctctt taaacacgtc
    24541 atacaccggt ttaatggctt tcgacgccat catggtggcg gtgccaactt cataaacctg
    24601 caaaatagcc ggagcgttgc cggtacgaaa tacggcaatc cccgcgctta aattctgttc
    24661 gtagttgcct ttataggtcg gtacaatttt gtaatccgga ttttcggcgt taaaacgttg
    24721 ggccagagaa tccacctctt tacccagttc cccttccata gaatgccaga acggaatggt
    24781 cgtcactgcc tgtgcattcc ccattaacgc cagtccgagc gccagtgctg aagctgtata
    24841 atgtaacggt ttcatcgttt atctctcttg ttgtaccgaa tgcgcgaatt cacgcgtttt
    24901 atgctcgcgg ggtaacatga catgctcgaa ttacagaaaa ataacttttt tgttacattt
    24961 gtaagatagt aaggtgtcag aaagatgaca aggcggtgac ggcgtgggtg agggaaaatg
    25021 ggagatgggg cacggataag cggaaaaata gaaggtctga atcaaactct acagattact
    25081 catcgtttca tgccggatgc ggcgtaaacg ccttatccgg cctacaagat cgtgcaaatt
    25141 caatatattg caactcaccc agtaggcctg ataagcgcag cgcatcaggc aattttgcat
    25201 ttgtcacctg tctcaaagga gtcttttgac tccttatcaa tcaacgtgtt attacccgcc
    25261 taaatacgca cttctcactg cttcgttcgc cagcagcgcg tcgccggtat cggagagcac
    25321 cacatggccg ttttccagca cgtagccgcg atccgccagc tttagcgcct ggttggcgtt
    25381 ctgctcgacg aggaagatag tcatcccctg ctcgcgcagc tgctcgatgg tgtcgaaaat
    25441 ttgctggatg ataatcggcg caagaccgag cgatggctca tcaagtaaca acaaacgcgg
    25501 attgctcatc agcgcgcgac caatcgccag catctgctgt tcaccgccgg acatggtgcc
    25561 cgcccgctga acacggcgct catgcagacg tggaaacagt tcatacaccc actttatgcg
    25621 ctcctgaaac tggtcacgtt cggcaaagaa gccgcccatc gccaggttct cttccaccgt
    25681 catccgcgag aagacgcgac gcccttccgg gacaatcgct accgcttcgc gcatgatttt
    25741 cgccgtctgc cagtcggtaa tgtctttatc atcaaacaca attcgcccgc tggtggcgcg
    25801 cggatcgccg cataacgtgc cgagcaaggt ggttttcccc gccccgttcg cgccaatgag
    25861 agtgacaatc tcgccctgat tgatatgcag gctcacctca tgcagcgcct ggattttgcc
    25921 gtagtgggcg ctgactttgt caaaggacaa catgactttt tccatcttat gcctcaccta
    25981 aataggcacg gatcacgtcc gggttattac ggatttgctc cggcgtaccg tttgccagtg
    26041 gcgtcccctg attgaccaca taaattcggt ctgaaatccc catcaccagc ttcatatcgt
    26101 gttcaatcaa caaaatagtg gtgttgtgat gattgcgcag ttcggcaatc agctcatcca
    26161 gctctttggt ttcttttggg ttaagacctg ccgccggttc gtcgagcatt aaaatctccg
    26221 gctgcgtcac catgcagcgg gcaatctcca gacggcgttg gtcaccatag gccaggttac
    26281 tcgcctgacg gttggcgtgt tccagcaaac caatgcgctc aagccaggtg gcggcgcggt
    26341 cgagcgcttc gctctgggcg cgacggaaag aaggcgtttt caacaggcca gagaacagcc
    26401 cggttttcag ttgctgatgc tgcgccacca gcaggttttc aattaccgtc atttcacgga
    26461 acagacgcac atgctggaag gtgcgcacca cgcccatgcg agcaatttgc tgccccggta
    26521 aaccttcaag atgctgatcg cgcagtaaaa tggtgccgcc ggtgggtttg tagaaaccgg
    26581 tcaggcagtt aaaaaccgtg gtttttccgg caccgttagg gccgattaac gagacgattt
    26641 cctgcgggta cagttcaaga ttgacgttgt tcaccgccag caggccgccg aagcgcatca
    26701 tcagaccgtt aacagataat aatggctgac tcatgcctgc tctcctttcg ctgcgccgtt
    26761 tttcagcttc agttgcgtgc gcgtcatggg cagcaaaccc tgcggacgcc agatcatcat
    26821 cagcaccatc aaaccaccga gcattaacat gctgtattcg ttgaaatcac gcatcaattc
    26881 gcgcgacacc accagcagaa cagccgccag aatcactgca aattgcgagc ccataccgcc
    26941 gagcaccact atcgccagca caaaggccga ttcagcaaag gtgaaggatt ccgggctgac
    27001 aaagccctga cgcgccgcaa acagcgttcc ggcaaaaccg gcaaatgcgg cgctgatggt
    27061 aaaagcagtc agcttgatac ggcgcgggct taagcccagc gaacgacagg cgatctcgtc
    27121 ttcacgcaac gcttcccacg cgcggcccag cggcatccgc agcaggcggt taatgacaaa
    27181 caggcttaac accaccagca gcaacgccac caggtagagg aagataacgc gatcggacgg
    27241 atcgtatttc aggccaaaga aattactgaa cgtgtcccag ccgccttcac gagcggtacg
    27301 gctgaactcg agtccgaaga gtgtcggttt cgggatctga ctgattccgt tcgggccgcc
    27361 ggtgatttca gtgttattga gcagcaatat gcgcacaatt tcgccgaaac caagggtaac
    27421 gatcgccaga tagtcaccgc gcagacgcag caccggaaaa ccgagcagga aacccgccgc
    27481 cgctgccatc agtccggcaa tcggcaaaca ggtccagaac cccagaccgt aatagtgatt
    27541 gagcagcgca aaagtgtaag cgccgatggc gtaaaaaccg ccgtacccca gcaccagcag
    27601 accagaaaga ccaacaacca cattcagccc gagaccgagg ataatgtaga tcatggtcag
    27661 agtggcgata tccaccgtcc cacgtgacac cataaacggc cacgccacca caagcaccag
    27721 cagcgccacg aggaacagct tttgcttcac cgtggaccca tcaatcgcgg gcagaataaa
    27781 cttcggcccg gaaacgcttt tcaacccttt ctggaaagcc ggtcgcaaaa gctggaagaa
    27841 aaagaccacc gccgtgccga taaacatcca ctgccaacgg atgtctgaag ccgtgtcgac
    27901 caccagtttg gtgccatcca gctccagttg tacgcccata aagacgcccg ccaggacaaa
    27961 gaacatcgcg gcagagagca gtgccattgc aatatgcatc ggtttcatac tttctctacc
    28021 tccgggcgac ccagaatacc ggtcggcatc accagcagca ccagaatcag cagggcgaat
    28081 gagaccacat ctttatattc cgtactcaga taggcagaag agagcgcctc cgcaatcccc
    28141 aggatcagcc cgccaatcat cgcccctgga atgctgccaa tcccaccgag caccgcagcg
    28201 gtaaaggctt tcatcccggc cataaagccg atataggggt taatgacgca gtagaactga
    28261 ccgagcagta cgccagccac tgccgccatc gccgcgccaa taacaaaggt caaagcaatc
    28321 acccggtcgg tgttgatgcc aagcaggctc gccattttca gatcttccgc gcaggcacga
    28381 caggcgcgcc ccatacggga atagcgaatg aaaatcgtca gcgccagcat ggcgaggaag
    28441 gtgacaatcc agatcaccgc ctgcatggtg gtaatagagg cagagaagtt ttcgctatgg
    28501 cccaccaccc actgaccgtt aaacaggctc ggcagcgcca cgtcgcgcga accttcggtc
    28561 aggctgacgt agttttgcag gaagatggac ataccgattg cggaaataag cgcaatcagg
    28621 cgcttagagt tacgcaccgg gcggtaagct acccgttcga tactccagcc gtaggcgctg
    28681 gcgatgacga ttgcgccgac gaatcccgcc gccaccagca gccagccggt atcaatgccc
    28741 atcatcatca gcgcggcgat gatcataaat gagacgtagc tgccaatcat ataaacctcg
    28801 ccgtgggcga agttgatcat gccgataatg ccgtaaacca tggtgtagcc gatggctatc
    28861 agcgcgtagg tactgcccag cgtgacgccg ttaaacatct gctgcaagaa atacaaaaac
    28921 tgctcagaca taaggtaacc tttctaaacc cgcccgcatt ttacgggcgg tgggatgatc
    28981 acttggctgc cgtggatgaa ccgtcggcgt gccactggaa gacaccaaaa tcaaatccct
    29041 taagatcgcc tttttcatcc cagttcagcg gcccaatcac ggtgtttgca ccgttagctt
    29101 ttaaatcttt caccagcgcc agcggctcat cgctgccggt acgctcaagg gcagttgcca
    29161 gagattgcac tgccgcgtaa gtgatccaga cataaggccc ggacggatct ttcttgtctg
    29221 ctttcagcgc atcaacgatg ccctggtttg ccggatcctg gtcatagcgt tttggcatag
    29281 tgaccaacat gccttcggcg gcatcaccgg caatgttcga cagcgatgcg ttaccgacac
    29341 cttccggccc cataaactgg gttttcaggc caacggaacg ggcttggcgc agcatctgcc
    29401 ccatttccgg gtagtaaccg ccgtagtaaa cgaagtcgat attttctttt ttcaggcggg
    29461 cgatcagcgc ggagaaatct ttctccccgg cggtaatacc gtcgaagaag acgacgttgg
    29521 cgttagccgc tttcagcccg tcctgcaccg aacgcgccag cccttcgcca tactgttgtt
    29581 tgtcgtgaat gatggcgatg cgctggggct tcaccgtctc aagaatgtat tttgccgccg
    29641 taggcccctg ggaagagtcc agcccggcag tacgcataat gtgttgataa ccgcgttggg
    29701 tcagctccgg gttggtcgct cccggcgaga tcatcagaat accttcgtct tcgtagatat
    29761 ccgacgctgg ctgggtagag gaagaacaca gatgaccaat aacgtattta atgccgtcgt
    29821 taacgatttt gttggcgacc gcaacggctt gtttcgggtc gcaggcgtcg tcatattcca
    29881 cgccaaccag cttgtcgccc ttaattcccc ctttgacatt aatgtcttta atcgcctgac
    29941 gcgcgccgtt aaattccata tcgccccact gggcaatcgg gccggacatc gcgccgacaa
    30001 cggcgacttt aatatcgtca gccatagcgg tgtgtgaaat tgccagtgca atcatccctg
    30061 cgatgatagt tttcgcattc cgtttcatag tcaaaaatcc ccattcgtga tgttgtgttg
    30121 ctttgttttt atgtgttaac aaatcagact gttctttttt tatactgcac tgtttttgcc
    30181 tgtctgatta aggggttagc gcagtatttt gtgatgatag cgattaaaat ccctattttt
    30241 cagtcgatta agaacagata atattctgaa tttattgata gataaacaga aaaaagtgcc
    30301 tttgtcagca taaaataacg gcacaaaggg cggaataatt cactatcatt caggggatta
    30361 tgctggacat ttttcattct ctaatgtttt aactttgtaa ttattgctgt taaaaaatta
    30421 atcacctgcc aaaagaaata aaaaagagaa agcctccgat taaattattt cgctacactg
    30481 gttccacttt tgtgatttac acgggttacc catgaagctg accatcattc gattagaaaa
    30541 ctttagcgac caggaccgga ttgacctgca aaagatctgg ccggagtatt ccccttcctc
    30601 tttacaggtt gacgataacc accgtatcta cgccgcgcgt tttaacgagc gtctgcttgc
    30661 ggccgtgcgg gtaaccttaa gcggcaccga gggagcactg gattctctac gcgtgcggga
    30721 agtcacccgc cgtcgcggtg tggggcaata tctgctggaa gaggttttgc gtaacaatcc
    30781 tggcgtttca tgctggtgga tggcggatgc aggcgtggaa gatcgcggtg tgatgacggc
    30841 gtttatgcag gcactgggat ttacggcaca gcagggcggc tgggagaagc gttaatcgtc
    30901 aagttgattt caaaagtgat gaccaacgag aaattgcctg atgcgctacg cttatcaggc
    30961 ctataatgtg tgctgcaatt tactgatttc tttggttctt gtaggccgga taaggcgttt
    31021 acgccgcatc cggcatgaag caacgtattc gatattagca atttggcggc aacccaaagt
    31081 tgccgattaa caattacttc gcatcggtcg ccgtgccgtt ggcgtgccag tcaaatacgc
    31141 cgaactcaaa gccctttaga tcgcctttct catcccaggt cagtggcccc atcacggtat
    31201 ccacggagtt cgcttttagg tatttggcga tttcagccgg atcgtcagac tgattcaggc
    31261 ccgcctgcaa agattgcagc gcggcgtagg tggtccagac gaatgcgccg cttgggtcct
    31321 gttttttcgc tttgatcgca tcaacaatgg gtttgttcgc cggaacctga tcgtagttct
    31381 tcggcttggt caccagcagc ccttccgctg attcgcccgc aatgttagac agcgaaacgt
    31441 tagccacacc ttccggcccc ataaactgag ttttcagccc tgccgcgcgt gcctgacgca
    31501 ggatttgccc catttccggg tgataaccgc cgtagtaaac gaagtcgata ttctcttttt
    31561 tcagacgcgc caccagcgtt gagaaatctt tttccccggc ggtgatacca tcaaagaaca
    31621 ccacgttagc attgcctttc ttcaggccgt cctgcaccgc tcgtgccaga ccttcgccgt
    31681 attgctgttt gtcgtgaacg atagcaatac gctgcggttt cactttctca agaatatatt
    31741 tcgccgccgt cggcccctgg tcggagtcca ggccggtggt gcgcaggatc agctgatagc
    31801 cacgggcggt cagctccggc gcggttgccg ctggggtgat cattaaaatg ccttcgtctt
    31861 cgtagatgtc agacgcaggc tgcgttgatg aagaacagag gtgaccaatc acatatttaa
    31921 tgccgtcgtt aacgactttg ttcgccaccg caaccgcctg tttcgggtca caggcatcgt
    31981 catattttac gatttgcagt ttgttgcctt taatgccgcc tttagcgttg atatccgcaa
    32041 ccgcctgctc tgcgccggta aactcctggt caccgtactg cgcaaccgga ccggacattg
    32101 cacccacgac cgcgacttta atatcttctg ccagagccat attgctgaat gccagcgcga
    32161 tacatcctgc cagtaacgct ttacccttta tgttcatcct gagaatcccc attcttctgg
    32221 ttattacgtg tgttgtgatg ttgttgttca gcactttatt tcgttttatg catgactacc
    32281 cgtgctttag cagcatactc tgctaaaaca tacccgattt ttatgatatt ggaatagcta
    32341 ttttgacagt ttattaacaa tctgcgtggg gattggcgtt ttgccggagg gggaattgat
    32401 tattgtagag gcccaaaaaa caaaaacccc ggactctcat ccagggttct ctgcttaata
    32461 gcggaaatta cgcttcaata gcggcgcgaa gttttttcat cgcgttcttt tccagttggc
    32521 gcacacgttc agcggaaaca ccgtaacggt cagccagttc ctgcaacgtg gacttgttgt
    32581 cttcgtccag ccagcgcgca cggatgatgt cctggctgcg ttcgtccaga ccatgcatcg
    32641 cgtcggtcag acggtttgcc gcctgctctt cccagttatc atcttcgatg ccgtcggcaa
    32701 agttagatga tttatcctgc agatagagca ccggtgccat cggctggctg tcggaatcgt
    32761 cgtcggaaga caggtcaaag gtcatgtcct gtgccgccat acgtgattcc atctcacgta
    32821 cgtctttgct ggttacgccc agttcacggg ccaccatttc gacttcatcc tggttaaacc
    32881 agcccagacg ctgcttggtt ttacgcaggt tgaagaacag tttgcgctgc gctttggtgg
    32941 tcgcaacttt gacgatacgc cagttacgca gaacgtattc gtggatctct gctttgatcc
    33001 agtggacggc gaaggagacc aggcgcacac ccacttccgg gttaaaacgg cgcactgctt
    33061 tcatcaggcc gatgttacct tcctgaatca aatccgcctg tggcaggcca tagcccgcat
    33121 aattacgagc aatatgaaca acaaaccgca ggtgagacag gatcagcgtt ttagctgctt
    33181 ccagatcgcc atggtaatgc agcttttcag ccagcgcccg ctcctcgtca gccgacaaca
    33241 tcggccacgc gttagctgcc cggatgtagg aatccaggtt gccaactggg gctaaagcta
    33301 aactttgcat tttgtcagtc attcaaatcc tctcaatcga tatcttctgg cgcttcagtg
    33361 gtagcaacaa ctgtgccaaa gcttaagagc aacgaggtta tcattcactg ttttatcaga
    33421 ccgtgatttt atccacaagt tcaatgcaag cttgtgaata aattacgcac aaaatgtgac
    33481 atagagatga aataccggga agagacaacg gggtctcttt ccctgctacg gaacccattg
    33541 cagggaaaga gtataacacg cttttattat tcaggcgtaa agtggcgtaa atgttgtacc
    33601 gtggcaagcc acgctgccac ccagccaatc atcgagcata ccagcagcaa tagcaggcat
    33661 tcatcgaacg ataaaccatt gatatcaaac ttcgttccga aaacctgtgt cacttccgcc
    33721 accgctgacg acaaacgcag taccagaatt tctgacaaaa ttaatgacaa caatgcgcca
    33781 gaaaatccca gcagcgcacc gccgtacagg aacgggcgaa ggatgaaacc atccgtcgca
    33841 ccaatcagtt tctgcacgtt gatggagtca cggcgagcaa agatgctcag acgtacgctg
    33901 ttgccgatga cgaggaacac tgccgccacc atcaacacgc cgatcatcgc cgaaacgcgc
    33961 ccaaccagcc cggtcaacgc cgccagacgg gcaaaccagc tgtcatccat ccgcacttcg
    34021 tcaataccgt taatctgcgt gatacgatca cgcagcgtat tgagtgattc cgtcccctga
    34081 aaatcgagtt tcgggatcac caccgccact gccggaagcg ggttttcttc cagcatatcc
    34141 agcgcaccac caaaaccaga ccagttacgg aactcgccca gtgcgtcttc acgagaaaga
    34201 tagttcactt tctccacgcc ttgctcggcc tgcaactgtg ccaccacgcc cgcagcggcg
    34261 tcatcgtcca gcgttttttg caagtaaaca gtgatttgcg gtgacggata atactgcgtc
    34321 gccgcctgat taacgttttt gtacaccata taacagacgc tgggcagcgt cagggagatg
    34381 gcgataacca tcaccgttaa aaacgtggcg aacggtttgc ttttcagatc ctgcaatgcg
    34441 ccgtggaagg catagcgcac ctgttcgttg aaaacgttgg ttttgcgatt taccggtttt
    34501 ggcgaggatt tcgcgcgttt cggtgcgtta cgaccgccgt cgcctgagcc gccgaccgat
    34561 ttacggaagc gatccagacg cccaccaaac tgccgaatat gattgattgc atcgcgctta
    34621 ttcatggccc acgcctccat gcaagtgacc atcgctcagg gtgagcatgc gataggaacg
    34681 ccgtgaaatc agattaatgt cgtgcgttgc catcaatacg gttaccccaa cgcggttaaa
    34741 ctcttcaaac agacgtagaa tcccttccga caacgcgtcg tccaggttac cggtcggttc
    34801 gtccgccagc agtaccgcgg gcttattcac caccgcgcgg gcaatgccaa cacgctgttg
    34861 ttcaccgccg gaaagctgaa tagggaagtt cttcgctttg tccagtagcc cgactttatc
    34921 cagtgccgcc gacacccggc gacgaatatc gtcaccgctg gcaccagcga taatcagcgg
    34981 gatcgccacg ttatcgtaga cagtacggtc catcagtaga tggtgatcct ggaaaatcat
    35041 gccaatctgg cggcgcagaa acggaacttc acggtttttc agacgcgtga tgtcatggcc
    35101 gctaaaccag attttcccgg cgctgggccg ctcaatccca cagatcagct tcaggagggt
    35161 acttttccct gcgccggaat gaccggtcag aaacgccatc tcacccggct gcatatggaa
    35221 cgtaacgccc tgcagcgcct gtctcccacc gagataagcc ttgctgacat gttcaaagcg
    35281 aatcattgtt aatcctctcg ggcaaaaagt gcctctataa agtcgtccgc cttaaacgga
    35341 cgcaaatcct caatacgttc gccgacacca atgtagcgga tagggatacc aaactggtca
    35401 gccaccgaga aaattacccc gcctttcgcc gtgccgtcca gtttcgttag cgtgatgccg
    35461 gttaagccaa cagcttcgtg gaacagtttg gcctggctta ccgcgttctg cccggtgctg
    35521 gcatcaatag tcagcataac ttcatgcggc gcttcaacgt cgagtttctt catcacgcgg
    35581 acgattttct tcaactcttc catcagatgc gatttgttct gcagacgtcc ggcggtatcg
    35641 gcaatcagga cgtcgatatt acgcgcttta gctgcctgaa tggcgtcgaa gataacagag
    35701 gcggaatccg caccggtatg ctgggcaatc accggaatat tgttgcgctg accccagacc
    35761 tgaagctgtt caaccgcagc tgcacggaaa gtatcgcccg ccgccagcat caccgattta
    35821 ccctgctgct caaactgacg cgccagctta ccaatcgtcg tggttttacc cacaccgttg
    35881 acgcccacca tcaggatcac gaacggcgtt ttatcttcaa cattcagcgg ctcatcgact
    35941 ttcgccagaa tctcgcccat ctcttctttc agcaggccat agagcgcctc ggcatcacga
    36001 agctgcttgc gggatgcgcc ttccgtcaga ttggtgataa ttttacgtgt ggtttccacg
    36061 cccacatcgg cgatcaacag ctgttcttcc agctcttcaa acagatcatc gtcgattttt
    36121 ttaccgcgga acaggctgat aaatccggaa ccgagatttt ctttggtttt taacaggctg
    36181 cgtttcaggc gcgcgaagaa gccttctttg gtcggttttt cttgctcctg ggcaatttct
    36241 gcaaccggct gctcttcttc tgccggagga actaccatca ccgcctcttc tgccgcttcg
    36301 gcagccagcg cctgtgcttc cagctcttcg tcggtgattt cttctttagc cgcttcttct
    36361 tctgccgctt cgacaatctc tacggtttcc gcttcagcct gccactcttc tggcgaaacc
    36421 tcttcggcgt tgacgtcttc cggcaacggc agctcttcac gttcgatagc cactggctcc
    36481 ggcgtttctt ctacgaccgg ttccggctgt gcaacaactt ccgcttcagg ctgcgctttt
    36541 tcactttcaa caacctgttc agtgacttcc acaacgtcgg cagcaaaagt ttccgcctcg
    36601 gcttcagtat gcgcctgcgg ctgctcttca acggcttgtt cagaagcctt cgcaggctct
    36661 tgcgcctgaa cgatttcttc tacaaccggt tgttcattct gaacttctgt ctctttttcc
    36721 ggggtctgct ctttttgacc aaagcccagc caggaaaaaa agccacgtct tttttctttc
    36781 gccatttgcg actacactcc tcgctgttgc ttcatggcac agcgttaacg ctatgtacat
    36841 agcagctaaa aaaatgatga aatagtctat cacttaactt aattcacatc accgcctgca
    36901 agtatgtgtt atctggcgga ttgagcaatt tatcatgaaa aaaccgaatc attccggcag
    36961 cggccaaatc cgcattattg gcgggcagtg gcgaggccgt aaactcccgg ttcctgatag
    37021 cccaggtctg cgccccacca ccgaccgcgt acgcgaaacg ttgtttaact ggctggctcc
    37081 ggtcattgtt gacgcccaat gtctggattg cttcgccggg agcggcgcgc tggggctgga
    37141 agcgttatcg cgctacgctg cgggggcaac gttgattgag atggatcgcg tggtttctca
    37201 gcagttaatt aagaatctgg cgacactaaa agcaggcaat gcacgcgtgg tgaacagcaa
    37261 cgcaatgtca ttcctggcgc aaaaaggcac accgcataat atcgtgtttg tcgatccacc
    37321 gttccgccgt ggcttgttag aagagacgat aaatttactg gaagataacg gctggctggc
    37381 tgacgaagcc ctgatttatg tcgaaagcga agtcgaaaac ggtctgccca ctgttccggc
    37441 aaactggtca ttacatcggg aaaaagtggc gggtcaggtg gcttatcggc tgtatcaaca
    37501 cgaagcacaa ggagaaagtg atgctgatta atattggtcg tttgttaatg ctctgcgttt
    37561 ggggattttt aatcctcaat ctggtgcatc ccttcccgcg cccgctaaat attttcgtta
    37621 acgtggcgct gatttttacc gtgctgatgc atggtatgca gctggcgcta ttgaaatcca
    37681 ctttaccgaa agatggcccg cagatgacca ccgccgaaaa ggtgcggatt ttccttttcg
    37741 gcgtgtttga actgctggtc tggcagaaga aatttaaagt taaaaaataa ccttattgtt
    37801 cgccaacaaa gctaacaaag cgcgttcctt tatagctcag cgtaccttta tcgcccactg
    37861 tcagggcgtg gtactgctgg gcgtcgaggc gaaacgtctg ctccattcct ccgctttgcg
    37921 gtttgaagct tgcctcatag cgcatactgg tgcctgccgg agtcacttcc tgctggcgcg
    37981 aacggcgatc gttaatcggt ttttcccgct tgttgctcac caccaccagc ttttgctgga
    38041 gcggagccat atcattatca gctttttccc gtcgctgttg cataaaacga aacgatgcgg
    38101 cgacgacaat taagccaatg ataacaataa agaaaagagg tggtttactc atctttatcc
    38161 ctcatcggaa aatgcggaat taagcatacc ctgccagtta tggtgttgtc atccgtccac
    38221 cctcgccact aaactggaag caagaccgta ggcattctgc ttacgaaaaa ataacgaatt
    38281 caaggaacta agatgctttg gtcgtttatc gctgtctgtc tttccgcatg gctatctgtg
    38341 gatgcatcgt atcgtgggcc aacctggcaa cgctgggtgt ttaaaccgtt aacccttctt
    38401 ctcctgctgt tactggcctg gcaagcaccg atgttcgacg ccattagcta tctggtgctg
    38461 gcgggactgt gcgcctcact gctgggcgat gcgctaaccc tgttgccacg tcaacgtctg
    38521 atgtacgcca tcggcgcgtt tttcctctcg cacctgctgt acaccatcta tttcgccagt
    38581 cagatgacgc tctctttctt ctggcctctg ccactggtgt tgctggttct gggtgcgctg
    38641 ctactggcga ttatctggac gcggctggaa gagtaccgtt ggcctatctg cacgtttatc
    38701 ggcatgacgc tggtgatggt gtggctggca ggtgaactgt ggttcttccg tccgaccgct
    38761 ccggcgctct ctgcgtttgt tggcgcttcg ttgctgttta tcagtaactt tgtctggctg
    38821 gggagccact atcgccgacg cttccgtgcg gataacgcga ttgctgcggc ctgctacttt
    38881 gccggtcact tcctcatcgt ccgctcactg tatctctgat aaaacttgac tctggagtcg
    38941 actccagagt gtatccttcg gttaatgaga aaaaacttaa ccggaggatg ccatgtcgac
    39001 acctgacaat cacggcaaga aagcccctca atttgctgcg ttcaaaccgc taaccacggt
    39061 acagaacgcc aacgactgtt gctgcgacgg cgcatgctcc agctcgccaa cgctctctga
    39121 aaacgtctcc ggcacccgct atagctggaa agtcagcggc atggactgcg ccgcctgtgc
    39181 gcgcaaggta gaaaatgccg tgcgccagct tgcaggcgtg aatcaggtgc aggtgttgtt
    39241 cgccaccgaa aaactggtgg ttgatgccga caatgacatc cgtgcccagg ttgaatctgc
    39301 ggtgcaaaaa gcgggctatt ccctgcgcga tgagcaggcc gccgacgagc cacaagcatc
    39361 gcgcctgaaa gagaatctgc cgctgattac gcttatcgtg atgatggcaa tcagctgggg
    39421 tctggagcag ttcaatcatc cgttcgggca actggcgttt atcgcgacca cgctagttgg
    39481 gctgtacccg attgctcgtc aggcattacg gctgatcaaa tccggcagct acttcgccat
    39541 tgaaacctta atgagcgtag ccgctattgg tgcactgttt attggcgcaa cggctgaagc
    39601 tgcgatggtg ttgctgctgt ttttgattgg tgaacgactg gaaggctggg ccgccagccg
    39661 cgcgcgtcag ggcgttagcg cgttaatggc gctgaaacca gaaaccgcca cgcgcctgcg
    39721 taacggtgag cgggaagagg tggcgattaa cagcctgcgg cctggcgatg tgattgaagt
    39781 cgccgcaggt gggcgtttgc ctgccgacgg taaactgctc tcaccgtttg ccagttttga
    39841 tgaaagcgcc ctaaccggag aatccattcc ggtggagcgc gcgacgggcg ataaagttcc
    39901 tgcaggagcc accagcgtag accgtctggt aacgctggaa gtgctgtcag aaccgggtgc
    39961 cagcgccatt gaccggattc tgaaactgat cgaagaagcc gaagagcgcc gcgcccccat
    40021 tgagcggttt atcgaccgtt tcagccgtat ttatacgcca gcgattatgg ccgtcgccct
    40081 gctggtaacg ctagtgccgc cgctgctgtt tgccgccagc tggcaggagt ggatttataa
    40141 aggtctgacg ctgctgctga ttggttgccc gtgtgcgtta gttatctcaa cgcctgcggc
    40201 gataacctcc gggctggcag cagcagcgcg tcgtggggcg ttaattaaag gcggtgcggc
    40261 gctggaacaa ctaggtcgtg ttacccaagt ggcgtttgat aaaaccggta cgctgaccgt
    40321 cggtaaaccg cgcgttaccg cgattcatcc ggcaacgggt attagtgaat ctgaactgct
    40381 gacactggcg gcggcggtcg agcaaggcgc gacgcatcca ctggcgcaag ccatcgtacg
    40441 cgaagcacag gttgctgaac tcgccattcc caccgccgaa tcacagcggg cactggtcgg
    40501 gtctggcatt gaagcgcagg ttaatggtga gcgcgtgttg atatgcgctg ccgggaaaca
    40561 tcccgctgat gcatttgctg gtttgattaa tgaactggaa agcgccgggc aaacggttgt
    40621 gctggtagta cgtaatgatg acgtgctggg tatcattgca ttgcaggata ccctgcgcgc
    40681 cgacgctaca actgccatca gtgaactgaa cgcgctgggg gttaaaggag tgatcctcac
    40741 cggcgataat ccacgcgcag cggcggcaat tgccggggag ctggggctgg agtttaaagc
    40801 gggcctattg ccggaagata aagtcaaagc ggtgaccaag ctgaatcaac atgcgcccct
    40861 ggcgatggtc ggtgatggta ttaacgacgc gccagcgatg aaagctgccg ccatcgggat
    40921 tgctatgggt agcggcacag acgtggcgct ggaaaccgcc gacgcagcat taacccataa
    40981 ccacctgcgc ggcctggtgc aaatgattga actggcacgc gccactcacg ccaatatccg
    41041 ccagaacatc accattgcgc tgggactgaa agggatcttc ctcgtcacca cgcttttagg
    41101 gatgaccggg ttgtggctgg cagtgctggc agatacgggt gcgacggtgc tggtgacggc
    41161 gaatgcgtta aggttgttgc gcaggagata aggcaaaccg atcgcaacat tgagcgcgat
    41221 cggtcccctc gcccctctgg ggagagggtt agggggtgag gaaaaggcgg catcgaagcc
    41281 aatcagcccc tatcaaccgc ctttacgaat caaataacga taaggcagtc catccgtctc
    41341 tttcgcaacc agttcgtgtt ccataaaggt acaaaaccca ggaatatcgc gggtagtggc
    41401 cggatcgtcg gcgataatca gcaacgtttc gccaggctgc atattgcgca cggttttgcg
    41461 caccatcatc accggttccg ggcagcgcag gccaagcgcg tcgagtgtgt ggtcagggct
    41521 ggaaaagaga tcggtcattt tcttctcatc acttaaaaaa acggcgctag tttacgccct
    41581 gtgagtccgt aagcaaacca ggttaacgat tgcgtgaaaa ttaaccattg cattgtcaac
    41641 gtaaagcagt atcatgcggc ggctcgaaaa aagggtaagc acgttattat gttaaggtaa
    41701 cagacgtgtc gtacgtattg ggttccctca ccccaatggt taatcaaaaa ggtacaatat
    41761 gaacgttttc tcgcaaactc aacgctataa ggcgttgttc tggttatcgt tatttcatct
    41821 tctggtgatc acctccagta actatctggt tcagcttccc gtctccattt ttggtttcca
    41881 taccacctgg ggcgcgttta gctttccgtt tatttttctt gctaccgacc tgaccgtgcg
    41941 tatttttggc gcaccgctgg cccgacgcat tatcttcgcg gtaatgatcc ctgcgttatt
    42001 aatctcctac gtcatctcgt cgctattcta tatgggttcc tggcagggat tcggcgcact
    42061 cgcccacttc aacctgtttg tcgcccgtat cgccaccgcc agtttcatgg cctacgcgct
    42121 ggggcaaatc ctcgacgtgc acgtttttaa ccgcctgcgt cagagtcgcc gctggtggct
    42181 ggcaccgaca gcgtccacac tgttcggtaa cgtcagcgac acgctggcct ttttcttcat
    42241 tgccttctgg cgtagcccgg atgcctttat ggctgaacac tggatggaaa tcgcgctggt
    42301 cgattactgt ttcaaagtgt taatcagtat cgttttcttc ctgccaatgt atggcgtatt
    42361 actcaatatg ctgttgaaaa gactggcaga taaatccgaa atcaacgctt tgcaggcgag
    42421 ttaaaggttc gttatcagag ttgtgataag atggatgaat gagccgttat ggccgtttat
    42481 cgaaaggaag aagtcaatgc gcaatctggt taaatatgtc ggaattggcc tgctggttat
    42541 ggggcttgcg gcctgtgatg ataaagacac taacgctacg gcgcagggtt cggttgcgga
    42601 aagtaacgct accgggaatc ccgtcaacct gcttgatggc aagttaagtt tctcgctgcc
    42661 agcggatatg accgaccaga gcggtaagct gggaacgcag gccaataaca tgcatgtctg
    42721 gtccgacgcc accgggcaga aagcagtcat cgtcatcatg ggcgatgatc cgaaagaaga
    42781 tctggcggtg ttggcgaagc gtctggaaga tcagcaacgt agccgcgatc cgcagctgca
    42841 agtggtaacc aataaagcca ttgagctgaa aggtcacaaa atgcagcagt tagacagtat
    42901 tatctccgcg aaaggccaga cggcgtactc ttccgttatt ctgggtaacg tgggtaatca
    42961 actgctgacc atgcaaatta cgctgccagc tgacgatcag caaaaagcgc aaaccaccgc
    43021 agaaaacatc attaatacgc tggttattca gtaagtttta agatgatgag gcggcctcag
    43081 ggacgtgttc cggaggccgt ttttttaatc gccacgtcag taataacgcg attgcgacca
    43141 gtcccgccgc cgccagataa atcaccggta cgcccgccca gctcatcacc agcccagcca
    43201 gtggtccagt cacgccaagc gataaatcca taaatacggt gtaagttgcc agcgccgccc
    43261 cctgattttg ctgcggaacc gcttttaccg ccactacacc caatgccggg aacaccagcg
    43321 aaaacccggc ccccgccagt aagacgccga ttttcgccat ccacggcata gtcgccacgc
    43381 caaccagtag caggccgatt atctcaacgc taaagcaaat catcgctacg tttaagccac
    43441 cgatacggtt aatgccgtta gggaataaca aacgcgtacc gacaaacgca cagctaaaca
    43501 gcgtcagcgc gaaagccgca ccgtcccaac ctttagcgtc ataaaacagc gtgataaagg
    43561 tggcgatgac gccaaatccg gcggaagcca gtgccagcgc cataccgtac agccagacgc
    43621 gcccaagcac cgcgcgaaac ggcagcggtt tgcctttact ggcttttacc gtcggacgcg
    43681 ggatcgccaa caaaatggcc accagcgcca cgcccataat gattaacgct aacgcctgca
    43741 agccgcccca gtgataaaac acgacgccta acggcgcacc catcgccatc gccccgtaag
    43801 tgacaatgcc gttccacgaa atcacccgcc cgatatgcag cgagccaacc actccaacgc
    43861 cccatagggt cgatcccgtt ccggcaaaac tttgcccaat cccaaggatg acgcgcccca
    43921 ggcaaagtaa taacaggctg atgacaggca gactggcggt taatcctgcc gtcagatacc
    43981 ccagaccgct caaaaagcag ccgcataaac cgaagacgac aatctttttg ggtcccagca
    44041 aatcggcgta acgtccggca tgagggcggc tcagcaaggt ggcgaaatat tgcaggctga
    44101 taaccaatcc tgcccagaag gcgctaaagc ccatcacatc atggacatag cccggtaata
    44161 cagcgagcgg caacccgatg gtgaggtagc tggcgaagtt aaacatgaca atggagacaa
    44221 tgcgcaaatt caggcgcaat ccgtttagcg cgggttcggc tacgggttcg ggcatgagga
    44281 tcaccacatt tttacaacag tgtttcattt ttaccatgtg ctgacgtgaa aatcagcagt
    44341 aagaatcaga atattgctgg cgtgactccc gcgctacact taacacaaaa agtcacaagg
    44401 aagccccaat ggaaacccct caacccgata aaacgggcat gcacattctg ctcaagttgg
    44461 cctcgctggt agtgatcctc gcgggcattc acgcagcggc agatatcatt gtgcagctgt
    44521 tactggcgct gttttttgcc atcgtcctca acccgctcgt tacctggttt attcgtcggg
    44581 gagtacaacg ccccgttgcc attacgattg tagtggtggt gatgctgatc gcactaaccg
    44641 cgctggtcgg cgtactggcg gcatcgttta acgaatttat ctctatgctg ccgaagttta
    44701 ataaggagct gacgcgcaaa ctttttaaat tgcaggagat gttgcctttt cttaatttgc
    44761 atatgtcgcc ggagcgaatg ctgcagcgga tggactcgga aaaagtggtt accttcacca
    44821 cagcgctaat gaccgggctt tccggggcaa tggcgagcgt gcttttgctg gtgatgaccg
    44881 tagtttttat gctgtttgaa gtgcgccacg tcccttacaa aatgcgtttt gcgctgaata
    44941 acccacagat tcacatcgca ggattacacc gcgcacttaa aggcgtttcg cattatctgg
    45001 cgttaaagac gctactcagt ttatggacag gtgtcatcgt ctggctgggg ctggcgctaa
    45061 tgggcgtaca gtttgcgctg atgtgggcag tactggcgtt tttgctcaac tacgtgccca
    45121 atatcggcgc ggtaatttcc gccgtaccgc caatgattca ggtgctgctg tttaatggtg
    45181 tttacgaatg tattctggtc ggcgcattgt ttttagtggt ccatatggtc atcggcaata
    45241 ttttagaacc acggatgatg ggccatcgcc tggggatgtc caccatggtg gtatttcttt
    45301 cattgttaat ctggggatgg ctgctcggcc cggtagggat gctactttcg gtgccattaa
    45361 ccagcgtgtg taaaatctgg atggaaacga cgaaaggtgg cagcaaactg gcgattttac
    45421 tggggccggg cagaccgaaa agtcggttac cgggatgagg cgacaagtga tacgatacgc
    45481 actttcattt tccattaaac gttggccctg atatgtatcg gatagttctg gggaaagttt
    45541 cgaccttaag cgcagctcca ctgccaccgg gtttacgcga gcaagcaccg caaggtccac
    45601 gacgcgaacg ctggctggcg gggcgtacat tgctttcgca cacgctttcc ccgctaccgg
    45661 agatcatcta tggcgaacaa ggcaaacctg cattcgcgcc ggaaacgccg ctatggttca
    45721 acttaagcca tagcggtgac gatatcgctc tgctgttgag cgatgaaggc gaagtcggct
    45781 gcgatatcga agtgattcgc ccgcgcgtca actggcgctg gctggcgaac gccgtgttca
    45841 gcctcgggga acacgctgaa atggacgccg tgcatcctga tcagcaactg gaaatgttct
    45901 ggcgcatctg gacgcgcaaa gaagccatcg ttaaacagcg tggcggcagc gcctggcaaa
    45961 tcgtcagcgt agatagcacc tatcactcct cgctgtcagt cagccattgt cagctagaaa
    46021 atttaagcct tgcgatctgc acccccaccc cctttacgct caccgccgac agtgtgcaat
    46081 ggatcgattc agttaactga tccgcccacc cgactgccca tctattgatc cagaacaggt
    46141 aatcagtatg acgaatactt aaaatcgtca tacttatttc cgccatctat tttaatccat
    46201 tggggttacc atgctctcca cactccgccg cactctattt gcgctgctgg cttgtgcgtc
    46261 ttttatcgtc catgccgctg caccagatga aatcaccacc gcctggccgg tgaatgtcgg
    46321 gccactaaac ccgcaccttt acacgcctaa ccagatgttc gcccagagca tggtttatga
    46381 accattggtg aaatatcagg cagacggttc ggtgatcccg tggctggcaa aaagctggac
    46441 tcattcagaa gatggtaaaa cctggacctt caccctgcgt gatgacgtga aattctccaa
    46501 cggtgaaccg ttcgatgccg aggcggcggc agaaaacttc cgcgcagtgc tcgataaccg
    46561 tcaacgtcac gcctggctgg agctggcaaa ccagattgtt gatgttaaag cactcaataa
    46621 aacagagctg caaattaccc tgaaaagcgc ctactatcct ttcctgcaag aactggccct
    46681 gccccgtcct ttccgtttta tcgctccctc gcagtttaaa aaccatgaaa ccatgaacgg
    46741 aattaaagcg ccgattggca ccggaccgtg gattttgcag gaatcgaaac tgaatcagta
    46801 cgatgtcttc gtccgtaacg aaaactactg gggcgaaaag ccagcgatta aaaagatcac
    46861 ctttaacgtc atcccggacc cgactacccg cgcggtggcg tttgaaactg gcgatatcga
    46921 cctgctgtac ggaaacgaag ggttattacc gctcgatacc ttcgcccgct ttagccagaa
    46981 tccggcttac cacacccaac tgtcacagcc gatcgaaacc gtgatgctgg cgctcaatac
    47041 cgccaaagcc cccaccaacg agctggcagt acgtgaagct cttaattacg cggtaaacaa
    47101 aaaatcgctg attgataacg cgttatatgg cacccagcag gtcgccgaca ccctgtttgc
    47161 cccttctgtg ccttacgcta atctcggcct gaaaccgcgc cagtacgatc cgcagaaagc
    47221 gaaagagttg ctggaaaaag ccggttggac gctgcctgcg ggcaaagaca tccgcgagaa
    47281 aaatggtcag ccgctgcgca ttgaactgtc gttcatcggc accgatgcgt taagcaaatc
    47341 gatggcggaa atcattcagg ctgatatgcg ccagattggc gcagatgtct cgctgattgg
    47401 cgaagaagag agcagtatct atgctcgtca gcgcgacggt cgttttggca tgattttcca
    47461 ccgcacctgg ggcgcgccat acgatccaca cgccttcctg agttcaatgc gcgtaccgtc
    47521 gcacgctgac ttccaggcac agcaaggatt agccgacaag ccgctgattg ataaagagat
    47581 cggcgaagtg ctggcgaccc atgacgaaac gcaacgtcag gcgctgtatc gcgacattct
    47641 gacccgtctg catgacgagg cggtttatct gcctatcagc tacatctcga tgatggtggt
    47701 atcaaaaccg gagctgggta atatccccta cgcgccgatc gccaccgaaa ttccgttcga
    47761 acagattaaa ccggtgaaac cttaatgttg cgttacgtat tacgccgctt tctgctgctg
    47821 atcccgatgg tgctcgccgc ctcggtgatc atttttctga tgctgcgcct cggtaccggc
    47881 gacccggcgc tcgattatct gcgtttatct aacctgccgc cgacgccgga gatgctggcc
    47941 tctacccgca ccatgctcgg gctggatcag ccgctgtacg tccagtacgg tacctggttg
    48001 tggaaggcgc tgcatcttga ctttggtatc tcattcgcca gccaacgccc ggtactggac
    48061 gatatgctga acttcctgcc cgccacgctg gaacttgcag gtgcggcgct ggtattaatt
    48121 ctgctcactt ccgtaccgct cggtatctgg gcggcgcgcc accgcgatcg tttgccggat
    48181 ttcgccgtgc gctttatcgc gtttcttggc gtgtcgatgc ccaacttctg gctggcattt
    48241 ttgctagtga tggcgttttc ggtgtatctg caatggctac ccgcgatggg ctacggcagc
    48301 tggcagcaca tcattttacc tgcggtttcc atcgccttta tgtcgctggc gattaacgcg
    48361 cgactgctgc gcgccagtat gctggacgtc gccggtcagc gtcacgtcac ctgggcgcgg
    48421 ctacgcggcc tgaatgacaa acagaccgaa cgccgccata ttctgcgcaa tgcctcgctg
    48481 ccgatgatca ccgccgtggg gatgcacatt ggcgaattga ttggcgggac gatgattatc
    48541 gaaaacatct ttgcctggcc tggcgtcggg cgctatgcgg tgtcggcgat ttttaaccgt
    48601 gactatccgg tgattcagtg ctttacgctg atgatggtgg tggtttttgt ggtctgtaat
    48661 ttgattgtcg atttgctcaa cgccgcgctg gacccgcgca ttcgtcgtca tgaaggagcg
    48721 cacgcgtgaa ctttttcctc tcttcccgct ggtcggtacg cctggcgctg atcattatcg
    48781 ccctgctggc gctgattgcg ctcaccagcc agtggtggca gccgtacgat ccacaggcaa
    48841 ttgatttgcc gtcgtgcctg ctttcgccgg atgcgcagca ctggctgggc accgatcact
    48901 taggtcgcga tattttctcg cggctgatgg cagcgacccg cgtgtcgctc ggttcggtaa
    48961 tggcctgcct gctgctggtg ctgacattag ggctggttat tggcggcagc gccgggttga
    49021 ttggcgggca cgttgatcag gccaccatgc gcgtcgccga tatgtttatg accttcccga
    49081 cctcgattct gtcgttcttt atggttggcg tgctcggcac cgggctgacc aacgtaatta
    49141 tcgccatcgc cctgtcgcac tgggcgtggt atgcacgcat ggtgcgcagc ttggtgattt
    49201 cactacgcca acgcgagttt gtgctggcgt cacggctttc cggtgcgggc catgtgcggg
    49261 tgtttgtcga tcatctggca ggcgcggtga tcccttcgct gctggtgctg gcaacgctgg
    49321 atatcggcca tatgatgctg cacgtcgcgg ggatgtcttt ccttggcctc ggtgtgaccg
    49381 cgccgaccgc cgaatggggc gtgatgatta acgacgcgcg ccagtatatc tggacccagc
    49441 cgctgcaaat gttctggccg gggctggcgc tgtttatcag cgtgatggcc tttaacctgg
    49501 tgggtgacgc actgcgcgat catctggatc ctcatctggt gacggagcac gcacactaat
    49561 gccgcaacag attgaactac gtaatatcac gctacaggcc gcgcagccgc tggtacacgg
    49621 tgtatcgtta accctgcaac gcgggcgcgt gctggcgtta gtcggcggta gcggcagcgg
    49681 gaaatcatta acctgcgccg cgacgctggg cattttgccc gctggcgttc gccagacggc
    49741 gggggaaatt ttagccgatg gcaaaccggt ttcgccttac gccctgcgcg gcatcaaaat
    49801 tgccaccatc atgcagaacc cgcgcagcgc ctttaatcca ctgcacacca tgcacaccca
    49861 cgcgcgggaa acctgcctgg cgttagggaa acccgccgat gacgctacgc ttaccgctgc
    49921 catagaagcg gtggggctgg aaaacgccgc gcgcgtgctg aagctgtacc cgttcgagat
    49981 gagcggcggc atgttgcagc gcatgatgat tgcgatggcg gtgctgtgtg aatcaccgtt
    50041 tatcatcgcc gatgaaccga ccaccgacct cgacgtggta gcacaggcgc gcatcctcga
    50101 tctgctggaa agcattatgc aaaaacaagc gccgggaatg ctgctggtca cccatgatat
    50161 gggcgttgtg gcgcgtctgg cggatgacgt ggcggtgatg tcacaaggta aaattgtcga
    50221 acagggcgat gtagaaacgc tgtttaacgc ccccaaacat acggtgacgc gcagcctggt
    50281 ttccgctcat ctcgccctct acggtatgga gctggcatca tgaccttact taacgtctgc
    50341 ggcctttccc atcactatgc ccacggtgga tttagcggaa aacatcaaca tcaggcggtg
    50401 ctgaataacg tttccctgac cctgaaaagc ggcgaaaccg tcgccctgct gggacgcagc
    50461 ggctgcggaa aaagcaccct cgcgcggctg ctggtaggct tagagtctcc ctcacaaggg
    50521 aatattagct ggcacggcga atcgctggcg aaacttaatc gcgcccaacg taaagccttc
    50581 cgccgtgata ttcagatggt gtttcaggac tccatcagtg ccgtgaatcc gcgcaaaacc
    50641 gtgcgcgaga tcctgcgtga accgatgcgc cacctgctat cactgaaaaa atccgaacaa
    50701 ctggcgcgcg ccagcgaaat gctgaaggcg gtcgatctcg atgacagcgt tctcgacaaa
    50761 cgcccaccgc agttaagcgg cggccagctc cagctcgtct gcctggcacg cgcgctggcg
    50821 gtcgaaccga aattgctgat tctggatgaa gccgtttcta accttgatct cgtgttacag
    50881 gcgggcgtca ttcgcctgct gaaaaagcta caacaacagt ttggcactgc ctgcctgttc
    50941 atcactcatg acttacgcct ggtggaacgc ttttgccagc gagtgatggt gatggacaac
    51001 ggacaaatcg ccgaaaccca ggcggtggga gataaattaa ccttttcctc tgacgccgga
    51061 cgtgtgctac aaaacgcggt attacccgca ttccccgtgc gccgtcgcac ctcagaaaag
    51121 gtttaacgcc aatgcaacga gtcaccatca cgcttgatga cgatttactg gagacgctgg
    51181 acagcctgag ccagcgtcgt ggttataaca accgttccga agctatccgc gatattctgc
    51241 gtagtgccct ggcgcaggaa gctacccagc agcacggcac acaaggtttc gcggtgctgt
    51301 cgtatgtgta tgaacacgaa aaacgcgact tagccagccg tattgtttcc acccagcatc
    51361 atcaccacga cctctccgtc gccacgctac atgtgcacat caaccacgac gactgtctgg
    51421 aaatcgccgt gttgaaaggt gacatgggtg acgtgcagca ttttgccgat gacgttatcg
    51481 cccagcgcgg cgtgcggcac gggcatttgc agtgcctgcc gaaggaagat tgagttattt
    51541 ctaaaacaag gaaagaaaag atattaactg aacttaattt ataaaattaa gttcagttgc
    51601 atagatagat cctgtaaagg cagatattct gcgtaatgta cgtcgctaaa atttataaat
    51661 gattcaaacg ctcattcagt gtttcaataa tataggtatt aacagagact tgttgctgcg
    51721 cagccgcatt attaaggcgt tcactcagcg attctggata gcgtaacgta aaggtcttaa
    51781 tcttttctgt acgcgcatac ggctcaatac ccgcagcttt acaatcctca agatactctc
    51841 gcagggaaag ctctccttct ttttgcagcc cctgaatact gtcagagaca aaatcacaat
    51901 aacctgacaa gcccaggaat ttgccacgaa atgcattcag ttctggcacg taggtaataa
    51961 cagctggttg cccggcaatc tccatagagt ttggcgtctt cagtttgatc atggttttac
    52021 ccctatactt agtaaccagt cacacacgct ttctacagcg cctttatcgg tatctggcga
    52081 cggatgaggc cgatggaaac acgcaacgct catatttagt atgaacttgc aacgcgaacc
    52141 tcttccttcc ttaatttctc cgcctaatgc tttaaccagt gactctatat cagaccactt
    52201 gatcccttga ggaacaggag ttttaaatat ttgatccaat gtattttttt gtttcttccg
    52261 taaagcctgt accttggttc tcatggcatg tccttatgac ttcactttat gacatcacag
    52321 tatgaagtca agtttccctt ccttacgcca ttgtcccaat cgtcttcctg aatcgcagca
    52381 gtgcgatggt gaaaaacgcg ccgccaattg ccatcagcgt caggaactgc ggccagacga
    52441 tttcgaatcc ggcaccccgg tagaggatgg cctgcgcgag gctaacaaag tgtgtcgtcg
    52501 gcatggtcag cataatgtcc tgcaccatct gcggcatact ttcgcgcggc gtggatccac
    52561 cggaaagcat ttgcagcggc agcagcacca gaatcaccag cagccccagt tgcggcattg
    52621 aacgcgctat cgtccccata aaaatgccga ttgacgtggt ggcaaacaga ctgagcgcca
    52681 cgcccagcat aaacagcggg atcgagcctt caatcggtac gcccagtaca cctttcacca
    52741 tcagcaccag cgataatccc gataccacca gcaccaccag ccccatcgac cagatcttcg
    52801 ccatcatgat ctcaaacggc gttatcggca tcaccagtaa atgttccacc gtgccgtgtt
    52861 cacgctcgcg gatcagcgcc gatccggtca atacaatcgc cagcatggta atgttgttga
    52921 tgatcgccat caccccgcca aaccacgcgg gatcaaggtt cgggttaaag cgcatccggg
    52981 tttccagcga taccaacggt tcgctgttat cacggtagcg cgcgacaaag ctgttcactt
    53041 caccgttgat aatattctgg atatacccat tgccggtaaa tgcctggctc atgcgcgtgg
    53101 catcgacgtt cacctgaata tccggctggc gtccggcgag gacatcacgc tgaaaattag
    53161 gcggaatatt tatcgcgaag gtatagcgtc cggcgtccag tccggcatcc atctcatcgg
    53221 cggtgatcat ctccggtggc aaaaaccacg gacgatagaa gctgttaacg atccggttcg
    53281 ataactgcga ttgatccata tcggcaatgg cgatcggcgc gaggttcaac gatcctggcg
    53341 taacggtcgc tgacgaatac accgacaccg taaacgagaa gacaatcagc gtcagcatcg
    53401 ctttatcacc gagcagactg cgcaactctt tgatacccag attaaaaata ttgcgtaaat
    53461 ggcgcatcat ccctcctgtt ttttcagcag caggatgctt aagcccatca ccagcgggat
    53521 ggctatcagc aacgggataa aaagttgcca caaatcagtc agatccagcg ctttcgagaa
    53581 cgttccgcgg gcgatagtca gaaaatgact ggtcgggtaa acctcgccga tccaacgtcc
    53641 aggcccttcc agcgaagcta ccggatcgat catcccggaa aactgtgtcg ccgggatcaa
    53701 tgtgataatc gccgttccga aaatggcggc aatctggctt ttcataaagg tggagatcag
    53761 cagccccatt ccagtggcaa tgatgatata cagcagcgcc gccaaggtga gcgtcaggaa
    53821 actgccttta tgcggtacgc caaacacaaa caccgacagg ccgcagagca ggaaaaagtt
    53881 cagcatcccc agcgcgatgt acggcagctg tttgccaagc aaaaattcgc tacgggtggt
    53941 gggggtcacg taaaggttga taatcgaccc tagctctttt tcccgcacca cgctaagggc
    54001 gcttagcatt gacgggatca tcatcagcag aagcgggatc accgccggaa caatcgctgg
    54061 caggcttttt acgtccgggt tatagcgata acgcgtctca atgttcatca gcccgctttg
    54121 gctggcgggt gtcgattgtc ggctcgccac atcctgaagc caactctggt gcattgcctg
    54181 cacgtaacct tttaccgttt cggcacgact gggcatcgct ccgtcgatcc agacgccgag
    54241 ttccacaggc gtaccacgcg cgatatcgcg cccgaaattg ggcgggatct caatggccac
    54301 cgtgatatcg cccgcacgca tccgacgatc aagctcgtca taactggtga gcggcggctg
    54361 ttcgataaag taacgggaac cggagaggtt gagtgtccac gcctggctac tgacggtctg
    54421 gtcgcggtcg agcaccgcaa agcgcaggtt ttccacatcc atactgatgc cgtaacccat
    54481 tatcagcatc aggatcaccg ttcccatcag cgccagcgtc gaacgtactg gatcgcgtcg
    54541 cagttccagc gcttcgcggc ggctgtagct aaacagacgg cgcaggctaa atccctgacg
    54601 cggcgcgtgg gtggtgtcgt gtaccacggg cggcgcttcg gcttcgttgc tctgccctgc
    54661 cgcttcctgc aaataggcga taaatgcctc ttccagactg gcggctccgc gtttctcaac
    54721 cagttcctgc ggtgtaccgc tggcaagcac ttttccggcg tgcatcagtg agatgcggtc
    54781 gcaacgttcc gcttcgttca taaagtgggt ggagatgaag atagtcactt tgtcctggcg
    54841 cgagagatcg accatcaact gccagaacat atccctcgcc accggatcga caccagaagt
    54901 aggctcatcg aggattaaca tctccgggcg atgaatcacc gccaccgcca gcgaaagccg
    54961 ctggcgaatg ccgagcggca atgactccgg cagaacatct tcaacgtcgt tgagcttaaa
    55021 acgctcgctc atttcagcca ctcgcgcggg aatttccgct tccgggatgt gaaacaaacg
    55081 ggcatgtaac tccaggtttt gccgcacggt gagttcgtta tagagcgaaa acgcctgcga
    55141 catatagccc acacgacggc gggtatcgat atcttttgga tcaaccggtt gcccaaatag
    55201 ccacgcctca ccttcgctgg cgggcagcag tccggtaagc attttcatgg tggtggattt
    55261 accgcagccg ttcgaaccaa ggaatccaaa aatctccccg cgtggaatgc ggaaattgac
    55321 gtgatcaacg gcaacgaagg aaccaaaacg catggtcaga tcgcgcgctt cgatggcaat
    55381 ctctgcgttt tcaggttgat acggtgggat cactaccgcc tgatgcgcct ggcgttgcgc
    55441 ttgcggtaac agattaataa atgcttcttc cagcgtggcg ctttgcgttt gctgccgcag
    55501 ctcttcggcg ctgccggttg ccagcacttc tccggcattc atcgctacca gccagtcgaa
    55561 gcgttcggcc tcttccatat aggcggtggc gaccagcacg ctcatattgc tctgccgctg
    55621 gcgaatactg tcgatcagat cccagaactg ggcgcgggag agcgggtcaa cccccgttgt
    55681 tggctcatca aggatcaaca gttccgggtc gtggattaac gcgcagcaca gcccaagttt
    55741 ttgcttcatc ccgccggaga gtttacctgc cggacgatcg cgaaacggtg ctaacccggt
    55801 gctggtcagc agctcattaa ttcgcgcttc ccgctccgct ttatcgtgac cgaacaggcg
    55861 agcgaaaaaa tcgacatttt catacaccga caaggtgtgg tagaggtttt tgcccagccc
    55921 ctgcggcatc caggcgatgc gcgggcagac gtctcggcga tgcttcgggt cgtgcatatc
    55981 gccacccagc accaccacat tgccctgctc aatgacgcgg gcaccggaaa tcagtgacaa
    56041 taaactcgac ttcccgacgc catccggacc aatcagcccg accatgcaac gggccggaat
    56101 atcgagagtg atattgttca gcgcaacggt ttttccataa tgctggctca cgcccgccag
    56161 ttgcgcgaca ggcgggacgg gaaccagttc cagatgcgtc attgcggcaa cctcaccacg
    56221 aggtcgtcag gccacggaag ttcttcattc acccgcaccc acgctacgcc cggtaaaccg
    56281 gttttgacat attccagatg ctgctggagt aattccggtg ggatacgcgc tttgacgcgg
    56341 aacatcagtt tcagccgttc atcgctggtt tcgacggttt ttggcgtgaa ctgggcgaca
    56401 ctggcgacaa aactgatggt tgcaggaata cgcagatctg gcgcggcatc gaggatcagc
    56461 cgggcttcac cgcccagttt cagcgtgccc gcctgttcgg ttggcaggaa gaaggtcata
    56521 tagacgtcgc tgagatcgac catattcagc acccgaccgc ctgccgccag cacttcccct
    56581 ggctcggcaa cccgatactg cacgcgtccg tcacgcgggg ctttcagttc actgtcatcg
    56641 atatctgcgg caatgcgccg ttcagtggct tgcgctgctt cgacccgagt ttgcgcctga
    56701 atgatattgg tgcgtgctgc ttctatagcc gctttagaag ccgaaacctg ggctttcgct
    56761 gattccagcg cagctcgggc gctctcagcg gcggcgcggt cgtcatccag ctgttgcgca
    56821 gaaatagccc ctcgttgggc gagcgagcgg gaacgcgtat gacgtttggc aacagagtcc
    56881 agttcggcct ggcgttgatt gaccagcgac tgtgcggcac gagtttcact ttgtcgttgc
    56941 tccagcaaag cctgcgcggc ggcaacggcg ctttgtgcct ctttgatttg cgcgatggct
    57001 tccagtcgct gttcctgcaa cacgcgagta tccatcttcg ccagcacttc gccttcgcga
    57061 acaaactggc cttctttcac cagaatggtg tcgatacgcc cggcaatttt gctggcgata
    57121 tccacttccg tcgcttcaat gcgcccattg ctgacagcaa agccttccgg cacacccgcc
    57181 gggcgcaaca gccaccacgc cacgacggca gccaccgcca gtgccccgac aacccaccac
    57241 gccagatggc gcttactctt atccataatc gacccgccat aatccctgta agcaaacgac
    57301 gcgtgttaca actgccgccg aacgacgttc agcggattct tccatccggg ataagaagga
    57361 aacgacaaat acgaccagcg cgcagagcat tgagcgccaa cggggaaggc ttagtgaaaa
    57421 cgggtagtaa tgttgtcatc atcagcattc ctggccgtaa atgaaacacg catactcata
    57481 agtgtgatct ataaaactgg ctgacaaaac ggtatccatc ctgttggatc gcatttcacg
    57541 cctgttcagt tgcaggaaca gagcattcct ttttacgttt tatcaacagt ctcaaccaga
    57601 tagtaacatt aatattcatg ttgtctatgg ttcagagctg taacattgtg cgccttctaa
    57661 tcaaaggcat aagtccattt ctgttttttt catgctggta agacttccag ggagtggtta
    57721 tctctggccc tgcaccaggg ttaatttggc aattatgcat gctgaacatt cttccagcat
    57781 gtttactgtc acgggcgtta agacctaaaa ctgtccccgt ttcacctatg cgttctctcc
    57841 caccatttcc gttatgtgca acaacttgat ttatacctaa catttgtgaa catgctaata
    57901 gtgcttcaag tttaggtctg taatctgtcg aaacagaaaa ataacgatta aaggatcgac
    57961 aagagatagc ccggctagtt ttaaactttt tgccattaat tatagcatga tgctttttat
    58021 taataatttc cgctaattcg accggatcga gagcattggt catttcatca atagagacag
    58081 ttatcggtcc tataatgtaa cgttttcgct gatcatcaaa agttatccca tgatgatttg
    58141 ccattatttt gtatttgggg tcatagaaac atacaggata atcttttatc ccgtcgtagg
    58201 tatcctcatc aagtggtttc atgcgcgtga agttttgcat atagttacca ttgcaattcg
    58261 tttcatgatt tccagctaat acatgaacat tttcattgat actcattatg cgttttaaca
    58321 aatcgatgat aaattgatcg ccacagatat tactaaatcg atcgccggtt tggtcgccaa
    58381 gaaacgtaca tggcgtgact gcgtctttat taataaccaa ataaggctga agactttcta
    58441 aaacattatc tcttctgttc agtatactga ttattgtatt aacattagtg tcaagttgct
    58501 cattaaagtt ctcccttagc aatacgttga gaacttcatc ttctatatta agcaactggc
    58561 taaggaaatt ttctccttca gccatgattc ccaaatgtcc tgttgcaaaa agcgcgtaca
    58621 aaactgcacc gactgagcca tcggtatcgc caaaataggt cggcctggta cttgtgggat
    58681 caacaggtga acaattggag aaataggttc tgtcagcgaa ctggggtttt ataattgttt
    58741 cgaatttaat atcaaataca ttgtattgcg atatttccca gttccagatg ggtttgtacg
    58801 actcagtaac tgcaacatca ttagcattat catttactgt catattatta agtaaataaa
    58861 caatgctgca ccaattaata tcggtattat caagagggca accgtaattt ttactattaa
    58921 ttgtcacaat aaactgataa cagttattcc tacgtagaat atgagcgcgc atgcccgata
    58981 gcatactata tttttctgaa tgattcgttt gtatggtaaa tgaaattttg tcatcttgaa
    59041 tagattgttg gaaacgttct atttgccaat catcaatttt ttccacaact tcaccgagca
    59101 ttgtcctggc aatacttgat atatcatcac ctataccatt tgcaatatac ccaaacagtt
    59161 ctttagtgaa gttggtaaca tgctcttgca ccatatcatt tgttgcgatt gtcgttgtac
    59221 tgccattagt gcctgccaca gttccaattt tcatctttta cctcatatta ttcaccgcga
    59281 ggaatacata gttaacattt tatcttatga catacacctc atattcgatg gggaaataat
    59341 tatattttgc acaaatcatt cagccttaaa gcataatatg aaatttctta tctcatagca
    59401 gagaaagtcc ctaaatattg tagaggcgtc atcagtacat acctctacaa tcgattaaga
    59461 aaacctatga aatactggca aaattaaaaa gagataccac agcactcatg aagttttatt
    59521 gaggttgaaa acaactaagc cctgggacac cttttttatc accacgttcg ttaggtgcta
    59581 tcttccaaga atttagcccc atggtataat tatcaatttt ttgatgctgg tctaccccta
    59641 atccataatg gccaaatttc tgtttagttt ttttgagcgt ctgtccacat ttgaagatat
    59701 cttcaggttt tggtctgaaa gcagtagtgt tatt
//
LOCUS       DAEBXN010000010        58573 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.010, whole genome shotgun sequence.
ACCESSION   DAEBXN010000010 DAEBXN010000000
VERSION     DAEBXN010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58573
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.010"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(<1..1100)
                     /locus_tag="KJL99_000598"
     CDS             complement(<1..1100)
                     /locus_tag="KJL99_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HBD4435456.1"
                     /translation="MKQKITDYMDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPIFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQLPGFEDFFIAVDP
                     KTDLSAVTLPRALPLLTPFQMDDNSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDDVAPGTVIITETNVPH
                     KDNIAYFGNGDDEAHMVYQFSLPPLVLHAVQKQNVEALCQWAQSLSLPSSKTTWFNFL
                     ASHDGIGLNPLRGLLPESEILALVEALQQEGA"
     gene            complement(1312..1659)
                     /gene="pspE"
                     /locus_tag="KJL99_000599"
     CDS             complement(1312..1659)
                     /gene="pspE"
                     /locus_tag="KJL99_000599"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HBD4435457.1"
                     /translation="MAIFIHRRTLTCLKKGLLALALVFSLPVFAAEHWIDVRVPEQYQ
                     QEHVQGAINIPLKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKELLSEMGYTHVENA
                     GGLKDIAMPKVKG"
     gene            complement(1702..1923)
                     /gene="pspD"
                     /locus_tag="KJL99_000600"
     CDS             complement(1702..1923)
                     /gene="pspD"
                     /locus_tag="KJL99_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HBD4435458.1"
                     /translation="MNTRWQQAGQKVKLGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(1932..2291)
                     /gene="pspC"
                     /locus_tag="KJL99_000601"
     CDS             complement(1932..2291)
                     /gene="pspC"
                     /locus_tag="KJL99_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HBD4435459.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(2291..2515)
                     /gene="pspB"
                     /locus_tag="KJL99_000602"
     CDS             complement(2291..2515)
                     /gene="pspB"
                     /locus_tag="KJL99_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HBD4435460.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLVDEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2569..3237)
                     /gene="pspA"
                     /locus_tag="KJL99_000603"
     CDS             complement(2569..3237)
                     /gene="pspA"
                     /locus_tag="KJL99_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HBD4435461.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            3404..4381
                     /gene="pspF"
                     /locus_tag="KJL99_000604"
     CDS             3404..4381
                     /gene="pspF"
                     /locus_tag="KJL99_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HBD4435462.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4501..5766)
                     /gene="puuE"
                     /locus_tag="KJL99_000605"
     CDS             complement(4501..5766)
                     /gene="puuE"
                     /locus_tag="KJL99_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HBD4435463.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINTLAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGECGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(5804..7084)
                     /gene="puuB"
                     /locus_tag="KJL99_000606"
     CDS             complement(5804..7084)
                     /gene="puuB"
                     /locus_tag="KJL99_000606"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HBD4435464.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWVRYGNKQLELLDVNAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSHMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(7086..8573)
                     /gene="puuC"
                     /locus_tag="KJL99_000607"
     CDS             complement(7086..8573)
                     /gene="puuC"
                     /locus_tag="KJL99_000607"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HBD4435465.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVRIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHTFIREGEGKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(8723..9280)
                     /gene="puuR"
                     /locus_tag="KJL99_000608"
     CDS             complement(8723..9280)
                     /gene="puuR"
                     /locus_tag="KJL99_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HBD4435466.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(9307..10071)
                     /gene="puuD"
                     /locus_tag="KJL99_000609"
     CDS             complement(9307..10071)
                     /gene="puuD"
                     /locus_tag="KJL99_000609"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HBD4435467.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITAWQHHIAEKQRL"
     gene            10283..11701
                     /gene="puuA"
                     /locus_tag="KJL99_000610"
     CDS             10283..11701
                     /gene="puuA"
                     /locus_tag="KJL99_000610"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HBD4435468.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGDLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYHVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            11850..12014
                     /locus_tag="KJL99_000611"
     CDS             11850..12014
                     /locus_tag="KJL99_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4435469.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            12004..13389
                     /gene="puuP"
                     /locus_tag="KJL99_000612"
     CDS             12004..13389
                     /gene="puuP"
                     /locus_tag="KJL99_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HBD4435470.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            13523..13768
                     /locus_tag="KJL99_000613"
     CDS             13523..13768
                     /locus_tag="KJL99_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HBD4435471.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            14081..15724
                     /gene="sapA"
                     /locus_tag="KJL99_000614"
     CDS             14081..15724
                     /gene="sapA"
                     /locus_tag="KJL99_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HBD4435472.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCNPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            15721..16686
                     /gene="sapB"
                     /locus_tag="KJL99_000615"
     CDS             15721..16686
                     /gene="sapB"
                     /locus_tag="KJL99_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HBD4435473.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKELFPATIELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            16673..17563
                     /gene="sapC"
                     /locus_tag="KJL99_000616"
     CDS             16673..17563
                     /gene="sapC"
                     /locus_tag="KJL99_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HBD4435474.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            17563..18555
                     /gene="sapD"
                     /locus_tag="KJL99_000617"
     CDS             17563..18555
                     /gene="sapD"
                     /locus_tag="KJL99_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HBD4435475.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            18557..19363
                     /gene="sapF"
                     /locus_tag="KJL99_000618"
     CDS             18557..19363
                     /gene="sapF"
                     /locus_tag="KJL99_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HBD4435476.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            19431..19784
                     /locus_tag="KJL99_000619"
     CDS             19431..19784
                     /locus_tag="KJL99_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HBD4435477.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            20152..20940
                     /gene="fabI"
                     /locus_tag="KJL99_000620"
     CDS             20152..20940
                     /gene="fabI"
                     /locus_tag="KJL99_000620"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HBD4435478.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTRESFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            21084..22211
                     /locus_tag="KJL99_000621"
     CDS             21084..22211
                     /locus_tag="KJL99_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HBD4435479.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLAIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETLSLHPISLSLLEKLLNSTRVNAQPDNQLAALLCARIN
                     GSPACFSTCMDSSNEYKKISTLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            22279..24213
                     /gene="rnb"
                     /locus_tag="KJL99_000622"
     CDS             22279..24213
                     /gene="rnb"
                     /locus_tag="KJL99_000622"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HBD4435480.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLTIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTSLDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISSEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(24291..24394)
                     /locus_tag="KJL99_000623"
                     /pseudo
     CDS             complement(24291..24394)
                     /locus_tag="KJL99_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24448..26422
                     /gene="pdeR"
                     /locus_tag="KJL99_000624"
                     /pseudo
     CDS             24448..26422
                     /gene="pdeR"
                     /locus_tag="KJL99_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            26570..26743
                     /locus_tag="KJL99_000625"
     CDS             26570..26743
                     /locus_tag="KJL99_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4435481.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKTPWEI"
     gene            26833..27582
                     /locus_tag="KJL99_000626"
     CDS             26833..27582
                     /locus_tag="KJL99_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBD4435482.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            27851..28069
                     /gene="osmB"
                     /locus_tag="KJL99_000627"
     CDS             27851..28069
                     /gene="osmB"
                     /locus_tag="KJL99_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HBD4435483.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(28195..28521)
                     /gene="yciH"
                     /locus_tag="KJL99_000628"
     CDS             complement(28195..28521)
                     /gene="yciH"
                     /locus_tag="KJL99_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HBD4435484.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGAVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(28521..29258)
                     /gene="pyrF"
                     /locus_tag="KJL99_000629"
     CDS             complement(28521..29258)
                     /gene="pyrF"
                     /locus_tag="KJL99_000629"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HBD4435485.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNCDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CDLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(29451..30620)
                     /gene="lapB"
                     /locus_tag="KJL99_000630"
     CDS             complement(29451..30620)
                     /gene="lapB"
                     /locus_tag="KJL99_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HBD4435486.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYSCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(30627..30935)
                     /gene="lapA"
                     /locus_tag="KJL99_000631"
     CDS             complement(30627..30935)
                     /gene="lapA"
                     /locus_tag="KJL99_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HBD4435487.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(31084..31848)
                     /gene="pgpB"
                     /locus_tag="KJL99_000632"
     CDS             complement(31084..31848)
                     /gene="pgpB"
                     /locus_tag="KJL99_000632"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HBD4435488.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            32018..32608
                     /gene="ribA"
                     /locus_tag="KJL99_000633"
     CDS             32018..32608
                     /gene="ribA"
                     /locus_tag="KJL99_000633"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HBD4435489.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(32672..35347)
                     /gene="acnA"
                     /locus_tag="KJL99_000634"
     CDS             complement(32672..35347)
                     /gene="acnA"
                     /locus_tag="KJL99_000634"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HBD4435490.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELKVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGEPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(35511..35606)
                     /locus_tag="KJL99_000635"
     CDS             complement(35511..35606)
                     /locus_tag="KJL99_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4435491.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(35720..35887)
                     /locus_tag="KJL99_000636"
     CDS             complement(35720..35887)
                     /locus_tag="KJL99_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4435492.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(35890..36054)
                     /locus_tag="KJL99_000637"
     CDS             complement(35890..36054)
                     /locus_tag="KJL99_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HBD4435493.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
     gene            complement(36349..37323)
                     /gene="cysB"
                     /locus_tag="KJL99_000638"
     CDS             complement(36349..37323)
                     /gene="cysB"
                     /locus_tag="KJL99_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HBD4435494.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGPPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            complement(37533..40130)
                     /gene="topA"
                     /locus_tag="KJL99_000639"
     CDS             complement(37533..40130)
                     /gene="topA"
                     /locus_tag="KJL99_000639"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HBD4435495.1"
                     /translation="MGKALVIVESPAKAKTINKYLGCDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            40510..40761
                     /locus_tag="KJL99_000640"
     CDS             40510..40761
                     /locus_tag="KJL99_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HBD4435496.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(40797..41846)
                     /gene="sohB"
                     /locus_tag="KJL99_000641"
     CDS             complement(40797..41846)
                     /gene="sohB"
                     /locus_tag="KJL99_000641"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HBD4435497.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            42066..42824
                     /locus_tag="KJL99_000642"
     CDS             42066..42824
                     /locus_tag="KJL99_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HBD4435498.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQINVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            42821..43411
                     /gene="cobO"
                     /locus_tag="KJL99_000643"
     CDS             42821..43411
                     /gene="cobO"
                     /locus_tag="KJL99_000643"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HBD4435499.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIRHAFDAGVKAQIGIDY"
     gene            complement(43451..44323)
                     /gene="rluB"
                     /locus_tag="KJL99_000644"
     CDS             complement(43451..44323)
                     /gene="rluB"
                     /locus_tag="KJL99_000644"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HBD4435500.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGSRRSGGRNNG"
     gene            complement(44424..45044)
                     /locus_tag="KJL99_000645"
     CDS             complement(44424..45044)
                     /locus_tag="KJL99_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HBD4435501.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(45041..45922)
                     /gene="yciV"
                     /locus_tag="KJL99_000646"
     CDS             complement(45041..45922)
                     /gene="yciV"
                     /locus_tag="KJL99_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HBD4435502.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            46060..46104
                     /gene="trpL"
                     /locus_tag="KJL99_000647"
     CDS             46060..46104
                     /gene="trpL"
                     /locus_tag="KJL99_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HBD4435503.1"
                     /translation="MKAIFVLKGWWRTS"
     misc_feature    46081..46174
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            46196..47758
                     /gene="trpE"
                     /locus_tag="KJL99_000648"
     CDS             46196..47758
                     /gene="trpE"
                     /locus_tag="KJL99_000648"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HBD4435504.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYIADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            47758..49353
                     /gene="trpD"
                     /locus_tag="KJL99_000649"
     CDS             47758..49353
                     /gene="trpD"
                     /locus_tag="KJL99_000649"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HBD4435505.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAN
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGKIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            49354..50715
                     /gene="trpCF"
                     /locus_tag="KJL99_000650"
     CDS             49354..50715
                     /gene="trpCF"
                     /locus_tag="KJL99_000650"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HBD4435506.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLL
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            50727..51920
                     /gene="trpB"
                     /locus_tag="KJL99_000651"
     CDS             50727..51920
                     /gene="trpB"
                     /locus_tag="KJL99_000651"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HBD4435507.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            51920..52726
                     /gene="trpA"
                     /locus_tag="KJL99_000652"
     CDS             51920..52726
                     /gene="trpA"
                     /locus_tag="KJL99_000652"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HBD4435508.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            53106..53285
                     /locus_tag="KJL99_000653"
     CDS             53106..53285
                     /locus_tag="KJL99_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HBD4435509.1"
                     /translation="MAEHRGSSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            53371..53871
                     /locus_tag="KJL99_000654"
     CDS             53371..53871
                     /locus_tag="KJL99_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HBD4435510.1"
                     /translation="MNMKTIEDVFIQLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            53917..54423
                     /locus_tag="KJL99_000655"
     CDS             53917..54423
                     /locus_tag="KJL99_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HBD4435511.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSIGGYV
                     FEQFEITCYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(54483..55121)
                     /gene="ompW"
                     /locus_tag="KJL99_000656"
     CDS             complement(54483..55121)
                     /gene="ompW"
                     /locus_tag="KJL99_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HBD4435512.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            55361..55450
                     /locus_tag="KJL99_000657"
                     /pseudo
     CDS             55361..55450
                     /locus_tag="KJL99_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            55478..56221
                     /locus_tag="KJL99_000658"
     CDS             55478..56221
                     /locus_tag="KJL99_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HBD4435513.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSELIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            56251..56790
                     /locus_tag="KJL99_000659"
     CDS             56251..56790
                     /locus_tag="KJL99_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HBD4435514.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLSKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            56895..57293
                     /gene="yciA"
                     /locus_tag="KJL99_000660"
     CDS             56895..57293
                     /gene="yciA"
                     /locus_tag="KJL99_000660"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HBD4435515.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(57333..58061)
                     /gene="tonB"
                     /locus_tag="KJL99_000661"
     CDS             complement(57333..58061)
                     /gene="tonB"
                     /locus_tag="KJL99_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HBD4435516.1"
                     /translation="MTSITLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAP
                     AQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPK
                     PKPVKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALS
                     RNQPQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRY
                     EPGKPGSGIVVNILFKINGTTEIQ"
     gene            58276..58572
                     /locus_tag="KJL99_000662"
     CDS             58276..58572
                     /locus_tag="KJL99_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HBD4435517.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
BASE COUNT        14063 a        15145 c        14644 g        14721 t
ORIGIN      
        1 aatgcacctt cctgctgtaa tgcctcgacc agcgctaata tttcgctttc tggaagcaag
       61 ccacgcagtg ggttcaaccc gatgccatcg tgagaggcga ggaagttaaa ccaggtcgtt
      121 ttactggagg gcaagctcag actttgcgcc cactggcaca gcgcctcgac gttctgtttt
      181 tgcaccgcat gtaacaccag cggcggcagc gagaactggt acaccatatg cgcttcgtca
      241 tcaccgttgc caaagtaagc aatgttgtct ttgtgcggaa cattggtttc ggtaataatc
      301 actgtacctg gtgctacgtc atcaataatc gaccgtaaca gcttgataat cagatgtgtt
      361 ttttccagat ggatgcagct tgttcccggc tctttccaca taaagccaac ggcatccagg
      421 cggacatact cagcaccttt ctcaaggtaa cagagtaaaa catccaccat cgccagcaac
      481 acttcagggc tacggtaatt caggtcaatt tgatcgtcac taaaggtggt ccataaatgg
      541 cgcgtggaat tatcatccat ttgaaacggc gttaatagcg gtaacgcacg cggaagggtg
      601 acggcactga gatcggtttt cggatcaacc gcaataaaga aatcttcaaa acccggaagc
      661 tgttgtaaat agtttttaaa ccattcactt tttgcagaca tatggttgca gacaaaatca
      721 aacattaaat gactgcattc accgagttgc tgaatatcct gccactcccc cgcttcactg
      781 gcgacctgat gataatcaat taccgaaaat ccatcatcag atgaccatgg gtaaaaaggt
      841 aataaatgaa catgtgaaaa aatgctttgc agccattgat ggtagaactg attaaatatg
      901 ggtagtggtt ttaaatcatt actgtgaaat tgatcggcat aggtaattaa cacgacatca
      961 ctttcatccc agtgtttttt acgtcgctgt gtaattaatc gtttcgcact ctcaagacgc
     1021 gttacaagtt tctgtaaatg agttgcggta aatgttccac cgtagatttc gtccatgtaa
     1081 tccgtaattt tctgtttcat gttgcctttc cgtggtagcg gtcccatgag gcgagagact
     1141 actccgcaga aaataaactg tcaattgagc acaagggttt tagtcacgca ggaaatcatt
     1201 ttttaccagg gaaaaagcgt atttgtgagc tgacgcaaat aatctatagg gatgtaaata
     1261 aggtgtgaca gaaaaaaaac ggcgcataag cgccgctcat ggtgaattct tttaaccttt
     1321 gaccttcggc attgcgatgt ctttcaggcc accggcgttc tcaacgtggg tatatcccat
     1381 ctcgctaagg agctcttttg cctgcccgga ctggcgtccg gcattgcaat acactttcac
     1441 ggtgtcgttt ttatccggaa cggcggtggc aatgcgctct ttcacttcct tcaggggaat
     1501 attgatggcc ccctgaacgt gctcttgctg atactgctct ggaacacgaa catcgatcca
     1561 gtgttcagcg gcgaaaacgg gcagtgaaaa caccagtgcc agagcaagta agcctttttt
     1621 taaacatgta agcgtccttc tatggatgaa aattgccatc cattatacgc ctgagattta
     1681 acaatttttg cgaaactccc ctcacctttt ataacgctgt gccagtttat tagcagcccg
     1741 acttaacagc ggttccagcg ccacagccag caacattttc agcggtcggc gagcaactga
     1801 ttttatcgcc cagcccgcca cacccgccgg gccatagcgc agtgcggtaa gaagtaccag
     1861 cttgcctgct aatttgaaac caagcttcac cttttgcccg gcctgttgcc agcgagtatt
     1921 cataactttc ctcacagttg acggaaacgg ctacgtaacg tgaaagtatc ggaagtgaca
     1981 taacgttcca tctcgcgtaa acgcgtttca cttgccgcca gttcacggtc gacttcatcc
     2041 agcaattcgc tgctggaagg tagctgctca ccaaaggcca tgttgtccgg cattggatca
     2101 agcgcaaatg acaaaatgat gtaagcaacc agggtaaaca gcgccagacc gaaaaaaatc
     2161 gacagcacca ccaggatacg caccagtttt accggtacat caaaatagtt ggcaatcccg
     2221 gcgcagacgc cgcggaccat gccctgctgt ggaatacgcc ataacttttt attgagatta
     2281 atgcccgcca ttagcgatcc ctccagttcg gatgctctgc atcaagaata gattccagcg
     2341 cctgaatacg ttcgcgcatc cgttttgctt catcaaccag ttgcgctaat cgctgctgct
     2401 cactttgcga caattcactg cgaccagaac gattgctgta atgcagccat aaccagatcg
     2461 gtaaaacaaa cagcacaaaa atggttaacg gaatagccag aaatagcgcg ctcataagta
     2521 ctccttagac gatgagcggc ggcgcgttcg gacgccgcct ggatgttatt attgattgtc
     2581 ttgcttcatt ttggctttta attgtgccag ttgttcgctg attgcatcat cggctttcag
     2641 ttcggcaaac tgatcgtcca gcgatttttg tttaccgaag ctgtggcttt ctgcttccgc
     2701 ttccatctgg tcaatacgac gttcgaaaga ttcgaaacga gccattgctt catccagttt
     2761 gccactgtcc agctgacgac gcacatcgcg cgacgagttt gccgcctgat gacgtaacat
     2821 caatgcctgc tggcgagcgc gtgtttcgct caatttgttt tccagctcgc caatctcttt
     2881 cttcatgcgt gccagcgtat cgtccaccag cgtcacttca tgttccaggg acttaatcag
     2941 atcggtcagt ttctgttttt caattaacgc tgcacgtgcc agatcctctc tctctttcag
     3001 cagcgccagt tcggcttttt cctgccattc aacttcacgc gccgacgctt gttcaatacg
     3061 gcgagtcagc tgtttctttt ctgccaacgc acgcgccgaa gtagaacgta cttcaaccag
     3121 tgtatcttcc atctcctgga tcatcagacg caccagtttc tgtggatctt ccgctttctc
     3181 taacagagcg ttgatgttgg cattcacgat gtcggcaaag cgagaaaaaa tacccataat
     3241 gttgtcctct tgatttctgc gttcaggatt gtcctgctga actgttaata caatttgcgt
     3301 gccaattttt tatctttttg atttataaag atctgattga agaatcaaca gcaacatgcc
     3361 aggatgaatt agcgaattac actaacaagt ggcgaatttc atcatggccg aatacaaaga
     3421 taatttactt ggtgaggcga acagctttct cgaagtgctg gaacaggttt cgcatctcgc
     3481 accgctggac aaaccggtgc tcatcatcgg cgaacgcggc accggtaaag agttgattgc
     3541 cagccgcctg cattatctct cctcccgttg gcaagggccg tttatttccc ttaactgcgc
     3601 ggcgttaaat gaaaatctgc tggattccga actgtttggt cacgaagcgg gggcgtttac
     3661 cggtgcgcaa aaacgtcatc ctgggagatt tgaacgtgcc gacggcggta cgctatttct
     3721 tgatgaactc gctacggcac cgatgatggt gcaggaaaaa ttattgcgtg tgattgagta
     3781 cggtgaactg gagcgcgttg gcggtagcca gccgttgcag gtgaatgtgc ggttggtatg
     3841 cgcgacgaat gccgatctcc cggcgatggt caatgaaggt acttttcgcg ctgacctgct
     3901 cgaccggctg gctttcgatg tagtgcaact gccgcccctg cgcgagcgcg aaagcgacat
     3961 tatgctgatg gcagaacact ttgccattca gatgtgtcgg gaaatcaagc tgcctctgtt
     4021 cccggggttt acggagcgcg ccagagaaac attgctgaat tatcgttggc cgggaaatat
     4081 tcgtgaattg aaaaacgtgg tggaacgttc agtgtatcgc cacggcacca gcgattatcc
     4141 gcttgatgac atcattattg atccctttaa acggcgtcca cctgaagaag ccatcgccgt
     4201 ttcagaaaat acctcgcttc cgacgctgcc gctggattta cgcgagtttc agatgcagca
     4261 ggaaaaagag ttgctgcaac tcagtttgca acaggggaaa tataaccaga agcgcgcggc
     4321 tgaattactg gggttaactt atcatcagtt ccgcgcgttg ttgaaaaagc accagattta
     4381 gcgaagttga gcaatacaac ttgccggatg cggcgtgaat gctttattcg gcatacataa
     4441 tcaatacgtc gtttgcaggc ctgataagcg cagcgcatca gacattattg cgttgggcta
     4501 ttaatcgctc agcgcatcct gcaaaatttt cattgccgca tcgaattgcg catccgggat
     4561 ggtcagcgga tacaggaagc gaatcacgtt gccgtatgcg ccacaggtca gcaggagcag
     4621 cccctgcgcc agcgcgcgtt gctggatttt ctgtgcaatc gccgctgacg gctcgcccgt
     4681 ttgcggatcg ttaaactctg ccgcaatcat cgaccccagg ccgcgtaccg cagcaatggc
     4741 cggaacgctt tctttggcat caatcaacgt gtttttgaga cgctggccca gttgattcgc
     4801 gcgttcgcag agtgattctt tgtcgataat attgagcacc gcgtgcgcgg cagccaccgc
     4861 cagcgggttc ccggcgtagg tgccacccaa cccgcccggc gcgggcgcgt ccataatatt
     4921 cgcgttaccg accacgcccg aaagcggcat cccgcccgcg aggcttttcg ccatcgtcat
     4981 taagtccggc ttatcggcgt aatgatccat ggcaaacagt ttaccggtac gcgcaaagcc
     5041 gctttgcact tcatcggcaa tcatcacaat accgtgctcg tcgcacaggc ggcgaatggc
     5101 ggcaaccagc tcttttggcg caacgttgaa accgcactcg ccctgcaccg gttcgaaaat
     5161 aatggccgcg acctgcttcg cttcgatgtc tgatttaaac aagcgttcga tggcgtcgag
     5221 ggagtcctgt gttgaaatgc cgtgtaaatc tgacggataa ggtacgtgat acaccgaacc
     5281 agggaacggg ccgaagccga ttttgtacgg cgcaactttt ccggtcagcg ccatggtcat
     5341 atatgtacga ccgtggaagc caccgctaaa cgcaatcacg ccaggtcgtc cggtatgggc
     5401 gcgagcaatt ttcaccgcgt tttccaccgc ttccgcgccg gtagtgaaga atgcggtttt
     5461 agcctgcccg ctcactgggg caagggtgtt aattttctcc gccagcgtga cgtagctttc
     5521 gtacggcaca atctgatacg ccgtgtgggt aaactgttgc aattgctgct ccaccgccgc
     5581 gaccagatca gggtggcgat gcccggtatt cagcaccgca atgcctgcgg cgaaatcgat
     5641 gtattcgttg ccctctacgt ccttcagcgt agcgttttct gccgactggg cgaagaagtt
     5701 acacatcacg ccaaccccgc gtggggtagc agaaagacga cgctgatgga attcattgtt
     5761 gctcatgttt cacacctttt gactcttcac tgttaatcgg aaattaaacg cccagacgat
     5821 cgcgcaggct gtaataagcc gcgcccatcg cggtaaatgg cacgcgcagc gtgcgcccgc
     5881 cggggaacgg gtaatgcggc agattggcga aggcatcgaa acgctcggcg tcaccgcgca
     5941 gcagttcagc aatcaaacgc ccggcaagat gggtacaggt gacgccgtgg ccgctgtaac
     6001 cctgcatgta atagatgttg gtatcaaggc ggccgaactg cggcatgtgc gacaaggtca
     6061 gcaggaagtt gcccgtccag cggtaatcaa ttttcacgcc cttcaactga gggaaggttt
     6121 ttagtagttt cggtaccaca agacgctcaa cgtcatccgg gttgcgtgca ccatacacta
     6181 cacccccgcc gtacagcagg cggttgtcgg cggtaagacg gtagtaatca agcagatagt
     6241 tacagtcttc cacacagtag tttttcggga tcagcgaacg ggctaaatct tccgacagcc
     6301 gttcggtggt gatcacctgc gtgccgcacg gcatgctgcg tttcgctagt tccggctcta
     6361 ctttatcgcc cagatacgca ttcccggcga caatcacata cttcgccgtc acctgaccat
     6421 tggcagtccg aaccacggct ggcgtggtgt gctggatctg cgtcacggcg gaaagctcat
     6481 acacgcgccc gccgttcagg cggatggcat cggcttcacc aatggcgagg tttagcgggt
     6541 gaatatgccc accgctgtga tccagcagcg caccagtgta gcgatcgctg gcgacctcgc
     6601 ggcgaatagc gttgacgtcc agcagctcca gctgtttatt gccgtagcgt acccagttct
     6661 ctttctgctc ttcgagtgtg gcgagttgtt tatcattcat cgccacaaac agaccgccgg
     6721 ggcggtagtc gcagtcaatc tgataacgtt tgatacgttc gcggatgatc tcgccaccct
     6781 cgaacatcat gctgccgagc atacgggcgg tgtccatgcc gtagcttttt tcgatcacgt
     6841 cgatgtcgcg gctgtaggag ttcacaagct gcccgccatt gcgcccgctt gcgccaaagc
     6901 cgatgcgtga ggcttcgaga accactacgt caaagcccgc ttccgccaga tgcagcgcgg
     6961 aggagagccc ggtatagccg ccgccaacca cgcaaacgtc gcaggtgatt gactcattca
     7021 gtgtgtcgaa tggtgcatat ttattcgcac tggcggcgta gtaactgctg gtatgttcgg
     7081 tcatttcagg cctccaggct tatccagatg gttttcagtt cagtgaattt ttcaagggca
     7141 tgcagggatt tgtcgcgacc gttgccgctc tgcttatagc cgccaaacgg cacggtcata
     7201 tcgccgtcgt tgtagttatt gacgaagacg gaaccggctt tcaggcgtcg gctcatgcgg
     7261 tgcgcgcggg agaggtcgcg cgtccatacc gccgcgccaa ggccgtactg gctgtcgttg
     7321 gcaagctgca gcgcctgatc ttctgatgtg aaacgggtga ccaccagcac cggaccgaaa
     7381 atctcttcgc ggcttaagga cgcattcggg tccacatcca cgaagatggt cgggccgatg
     7441 gccgctgcca gcccggcgtt gcgaccatcc agcagcagtt gccccttgcc ttcgccttca
     7501 cgaataaagg tgtgtaccga gtcagcgtga gcgctgtcga ttaaggtgcc catggtggtt
     7561 gcgggatcaa gtggatggcc cggctgccag ttttgcgcct gctgttttaa cagggctaag
     7621 aattcatcgg cgatgctctc ttccagtaac aaacgcgttc cggcgatgcg cacctggccc
     7681 tggttgtaga agatgcctgc ggcggtggcg cttgccgctt tttgcagatc cggacaatcg
     7741 gcgaaaacga tgttggcgct tttgccgccc gcttccagcc agacacgttt catgttgcta
     7801 tcacccgcgt ctttcagcag ttgtttaccc gtacgcgtgg agccggtaaa ggcgatggca
     7861 tcgatatcgt tatgacgcga aagcgcctgc cccgcttcat gaccaaaacc cgtcaccacg
     7921 ttcaacacac catccggcaa gcctgcttct ttcgccagcc ccgcgagacg aatcgcactg
     7981 agcggtgatt tttcagacgg ttttagaatc acgctgtttc ccgccgccag cgccgggccg
     8041 agtttccagc aagtcagcaa cagcgggaag ttccacggca cgatggcggc aatcacgccg
     8101 accggttcac gcacgatcat cgccagctca tggctactgg tggtcgccac ttcgccatac
     8161 actttgtcga tcgcttcggc gtaccagcga atggcgcgcg ccgcgccggg aatatcatca
     8221 cgcagactgt gacgaatcgg tttgccggtg tcgagagttt ccagcagtgc cagctcttcg
     8281 gcgtgggctt ccattaaatc ggcgagttta ttcagtaccg ctttacgttt agccggagaa
     8341 gagagtgacc agtcgccgcg ttcaaatacg ccgcgtgctg cgctcaccgc acggtcgata
     8401 tcgacgctct tgccgcgggc aattttcgcc agcggtgcct gggtgaccgg atcaacggtt
     8461 tcaaaggttt cattttccgc cgcagcagta tattcaccgt taataaataa gcggttttca
     8521 atggcgagac ttaacgcttt atcctgccag taagccagat gatgaaaatt cattatgact
     8581 cctgtttcac gtctatcaga tatatgcagt ttgtgaacgt cagggcgcag agatccccgg
     8641 gctgcatgcc cgtttatttc tggcgacatt ctttatggat tgccgggcgc attgcgctta
     8701 cccggcttac agaacaaaaa gattaaaacg tggtgggcgt atgggcgctg ataattcggc
     8761 aaatacctgc cgacgtatta ctgaaactgt gcgggatgcc ggtattaatg gcataacttt
     8821 gccccgcgac gaggtggtaa tcctgaccat taatcgtcag aacaatttca ccttccagta
     8881 ctgtgcctat ttcctcaccc tgatgcttaa ttctttcccc agtggttgtg cccggctggt
     8941 acgtttcaaa gatcatcgcc agcgtgcgat tcgggttacc gttatgaacc agcttcattg
     9001 acacaccctg actgcccatc tcaattaagt cgtcctgatt aatgacgacc tgcggctcat
     9061 caggtttttc cggctcggaa aagaattccg agagtgacag accatacacc ttcagcagct
     9121 tttgcagcgt actgatggca gggctgactt tatcttgttc tatcgtactg atagcactgt
     9181 gagtcagccc ggagagttcg gcggcacgac gttgtgaaag cccctgctgc tggcggattt
     9241 ccgacaagcg ttttcctggc gccagtccct catcactcat atttgcattt ccttaaactg
     9301 tagtggtcag agtcgctgtt tttcagcgat atggtgctgc caagcggtga taaagccctc
     9361 gaacaatata cgcgaaagcg cgtactcgct actgttccat tccgggtgcc actgtacgcc
     9421 cagcgcaaaa ggatgattga tgacgctaac cgcctcaact aagccatccg gcgagcgagc
     9481 ttcaacacgc aaccgtgggc taacgacctt cgccccttgt ccatgtagag agtttaccca
     9541 aaagttgcta cattcaggta acaacgcaga cagtaatccc ccctcttcaa cctgaacttc
     9601 atgcgacggt gcatattgct gttccaccgg cagttcggga tcttcccgat gttccagcaa
     9661 ttcaggctgt tcgcacagct tgcgatgcaa cgaaccaccg gttgccacca ccagttcttg
     9721 taaaccccgg cagatggcga aaatggggat gcgcctttcg agtgcggcat taattatcgc
     9781 catgctcaga agatcacgcc cgggatcggc gtcaggctca tcgccgtttt caccatatag
     9841 gtgcggctgc acattgctgg gactactagg aagataaatg ccatcgagtt tcggcaaaag
     9901 ttgttcaagt aacgacggtt ccgccagcgc atgtggtagc gcaattggca agccgcctgc
     9961 gtggatgatg gcattcaggt acttttcttg cagagtctgg gtcgcatgac ccttaagcct
    10021 gttcctgcac attacgacac cgataaccgg attgttcatt atattttcca tgctcaatct
    10081 cacaaaatgg actaaattat cgccattact gcgctttaaa tgacctttat gttcaatatt
    10141 ttttcaatct agcagtggat tttcattttt gcaaactcaa tttaacattt gacaaacatt
    10201 tggtttgcat acagattcga atgatggtca ttatatttta cgctttgata acgagcggaa
    10261 aacaaaccaa aggcgaagaa tcatggaaac caatatcgtt gaagtagaga actttgttca
    10321 gcagtcagaa gagaggcggg gtagcgcctt tacgcaggaa gtgaaacgct acctggagcg
    10381 ctacccgaat acgcaatatg ttgatgtttt acttactgat ttgaatggtt gcttccgtgg
    10441 aaaacgtatc cctgtttcaa gcctgaagaa gctcgagaaa gggtgttatt tcccggcctc
    10501 ggtatttgca atggatattc tgggcaacgt agtagaagaa gcaggtctgg gtcaggaaat
    10561 gggcgagccg gatcgtacct gtgttcctgt tctcggttcc ttaactcctt ctgccgccga
    10621 tccagagttt atcggtcaga tgctcctgac catggtcgat gaagatggcg ctccctttga
    10681 cgttgagccg cggaacgttc ttaaccgcct gtggcagcag ctgcgccagc gcggattgtt
    10741 cccggttgta gcggtagagc tggagttcta tttactggat cgccagcgcg acgctgaagg
    10801 ggatctgcaa ccgccctgcg cgcctggcac cgatgaccgc aatacacaaa gccaggttta
    10861 ctccgttgat aacctcaacc acttcgccga cgtgctcaat gatattgatg aactggcgca
    10921 gttgcagctg attccggcag atggcgcggt cgctgaggcc tcgccgggtc agtttgaaat
    10981 caacctttac catactgata acgtgctgga agcctgcgat gatgcgctgg cactaaaacg
    11041 cctagtgcgt ctgatggcag aaaagcataa gatgcacgcc acttttatgg cgaagccgta
    11101 tgaagagcac gcgggcagcg ggatgcatat ccatatcagt atgcaaaata atcgtggcga
    11161 gaatgtgctt tctgacacag aaggcgaaga ttcgccgctg ctgaaaaaga tgctcgccgg
    11221 gatgattgac ctgatgccgt cgtcgatggc gttgctggca ccaaacgtga actcgtatcg
    11281 ccgcttccag ccgggaatgt atgtgccgac gcaggcgtcg tggggccata acaaccgcac
    11341 cgtcgccctg cgtattccgt gcggtgaccg tcataatcac cgcgtggaat atcacgtggc
    11401 gggtgccgat gccaacccat atctggtgat ggcagcgatt tttgccggta ttttgcatgg
    11461 ccttgataac gagctgccgt tgcaggaaga agtcgaaggc aacgggctgg aacaggaagg
    11521 cttacccttc ccgattcgcc agagcgatgc cctgggtgag tttatcgaga atgatcacct
    11581 gcgccgctat ttaggcgaac ggttttgcca tgtgtatcac gcctgtaaaa atgatgagct
    11641 gttgcagttt gagcgcctca tcacagaaac agaaattgag tggatgttga aaaacgcgta
    11701 attccccttg cggggttgtt tcatcggcct gagcggagcc aggcctgaca tttgctggta
    11761 atccagacat ttcccggatg tcgcgttatg gagcgcaggc ggcaacgggc ttccgttaac
    11821 gattaagact gtgacgagga aatgagatga tgccgatgat taaatccccg catggcgagg
    11881 gaggctgcgt atgcgcccct cctgctactg actggactcc gccccctttg cttcccttgc
    11941 taaacaggtt tgatttcagg tcgacacgac cgcaaacctt attacgcagg ggaggcagca
    12001 attatggcta ttaattcacc actgaatatt gctgcgcaac ccggcaaaac ccgtctgcga
    12061 aaatcactga aattgtggca ggtggtaatg atgggtctgg cctatctcac gccgatgacc
    12121 gtatttgata cttttggtat tgtctccggc attagcgacg gtcacgttcc ggcgtcctat
    12181 ttgctggcgc tggcgggcgt gctgtttacc gctatcagct acggcaaact ggttcgccag
    12241 tttccggagg ccggttcggc ctatacctac gcgcaaaagt cgattaaccc gcacgtcgga
    12301 tttatggtcg gctggtcatc actgctggat tatctctttt tgccaatgat caacgttttg
    12361 ttggcgaaaa tctatctctc cgccctcttc ccggaagtgc cgccgtgggt gtgggtggta
    12421 accttcgtcg ccattttaac cgccgcgaat ctgaagagcg tcaacctggt cgctaacttc
    12481 aataccctgt ttgtactggt gcaaatctcc atcatggtgg tgtttatctt cctggtggtt
    12541 cagggactgc ataaaggaga aggcgttggc accgtctggt cacttcagcc gtttatcagc
    12601 gagaacgcgc acctgatccc gattattacc ggtgcgacga ttgtctgttt ctcgttcctc
    12661 ggtttcgatg cggtgaccac gctttcggaa gagacgccgg acgccgcacg cgtgatcccg
    12721 aaagccatct tcctgacggc ggtatacggc ggcgttatct ttatcgcggc gtcgttcttt
    12781 atgcagctgt tctttcccga tatcagccgc tttaaagacc cggacgccgc actgcctgaa
    12841 attgcgctgt acgtcggcgg caagctcttt cagtcgattt tcctctgcac cacgtttgtg
    12901 aacacattag cgtctggcct ggcttcacat gccagcgtgt cgcgtctgct gtatgtgatg
    12961 gggcgtgaca atgtgtttcc ggagcgcgtg tttggctatg tgcacccaaa atggcggact
    13021 ccggcactca acgtcattat ggtcgggatt gtcgcgctgt cggcgctgtt ctttgattta
    13081 gtcaccgcga cagcactgat taacttcggt gctctggtgg cgtttacctt cgtgaatctg
    13141 tcggtgttta atcatttctg gcggcgtaaa ggaatgaata aaagctggaa ggatcacttc
    13201 cactatttgc tgatgccgct ggttggcgcg ctgacggtgg gtgtgctgtg ggttaaccta
    13261 gagtcaacgt cactgacact cggtctggta tgggcttcgt taggcggcgc atatttgtgg
    13321 tatctgatcc gccgctatcg caaagtgccg ctgtacgaag gtgacagaac gccggtgagc
    13381 gaaacgtaat agccggatgc gcggcgcatt atcctgcctg agggtaacgc gccgcgccat
    13441 taactataaa aagatgtatg acccggatca cgagtgctat catccgccct tatgcttagc
    13501 gcattaaccc caggggcaaa taatgaacca cgatatcccg ctaaaatatt ttgatattgc
    13561 cgatgagtac gcgaccgaat gcgcagagcc tgtcgcagag gctgaacgca caccgctggc
    13621 ccactacttc cagttactgc tcacccgttt gatgaacaat gaagagatca gcgaagaagc
    13681 ccaacatgaa atggctgctg aagcggggat taatcccgta cgcattgatg agatcgcaga
    13741 gtttttgaat caatggggta atgagtaatt atttaattgc agacgatcgg tcagtatcgt
    13801 tgtccggata aagcgttcac gccgcatccg gctaacaatg acacgcactg cagcgtgctt
    13861 taatgggttt ctcacgatcg taccgctctg ctcctcgcta agttgttctg tgagttatag
    13921 ttatagagaa atcacgggta cgaatgttga acagatggcg acgccacctg ttcaacgacc
    13981 aaccacacgg ccctcattac gacccctgaa cacaaacggt aatggcaaat gtcattaaat
    14041 attagtaaga tagcctcttt acataaaatc ccttaatatt atgcgccagg tattatcgtc
    14101 tcttttggtg attgctggac ttgtgagtgg tcaggcaatc gccgcgcctg aatccccccc
    14161 gcatgctgat atccgcgaca gcggttttgt ctattgcgtc agcgggcaag tcaacacctt
    14221 taacccatcc aaagcgagca gtgggttaat tgtcgatacc cttgccgccc agttttatga
    14281 tcgactgctg gatgtcgatc cctataccta tcgcctgatg ccggaacttg ccgaaagctg
    14341 ggaagtactc gacaacggcg cgacctatcg cttccacctg cgtcgcgatg ttccgtttca
    14401 aaaaaccgac tggtttactc ccactcgtaa aatgaatgcc gacgatgtgg tgtttacctt
    14461 ccagcgaatt tttgaccgca acaacccgtg gcataacgtc aacggcagca acttccccta
    14521 cttcgacagc ctgcaatttg ccgataacgt gaaaagcgtc cgcaaactgg ataatcatac
    14581 cgttgagttc cgactcgccc agccggatgc ttcttttttg tggcacctcg caacccatta
    14641 tgcttcggtc atgtcggcag aatatgcccg gaagttagag aaagaagatc gccaggagca
    14701 actcgaccgt caaccggtcg gcaccggtcc gtatcagttg tcggaatacc gcgccgggca
    14761 atttattcgc ctacaacgtc atgatgactt ctggcgcggt aaaccgttaa tgccgcaggt
    14821 agtggtggat ttaggctccg gcggcaccgg acgtctgtcg aaactcctga ccggggaatg
    14881 cgacgttctg gcctggcctg ctgccagcca gctatccatt ttgcgtgacg acccgcgctt
    14941 gcgtttaacg ctgcgtcctg ggatgaacgt cgcctatctg gcatttaaca ccgccaaacc
    15001 gccgctaaat aatcccgctg tccgccatgc actggcactg gcgattaata accagcgcct
    15061 gatgcaatcc atctattatg gtacggctga aacggcggcc tctattttac cgcgcgcctc
    15121 gtgggcctat gacaacgagg ctaaaattac tgaatacaat ccggcgaaat cgcgcgaaca
    15181 gttgaaggcg ttggggctgg aaaacttaac gctgaaactg tgggtgccca cacgttcgca
    15241 ggcgtggaac cccagtccac tgaaaactgc cgaactgatt caggcggata tggcgcaggt
    15301 tggcgtaaaa gtggtgattg tgccggtaga aggtcgcttt caggaggcgc ggttgatgga
    15361 tatgagccat gatctgacgt tatccggttg ggcgacggac agtaacgacc cggacagttt
    15421 cttccgtccg ttactgagct gcgcggcaat tcattcacag accaacctcg cccactggtg
    15481 caatccgaaa ttcgacagcg tgttgcgtaa ggcgctctcc tcgcagcagc tggcagcgcg
    15541 tattgaagcc tatgacgaag cgcagagtat tctggcgcag gaattaccta ttctgccgct
    15601 ggcgtcgtca ttgcgtttgc aggcctatcg gtacgatatc aaaggtctgg tacttagccc
    15661 gtttggtaac gcctcctttg ctggggtgta tcgcgagaaa caggatgagg tgaaaaaacc
    15721 atgattatct tcaccttacg ccgcattttg ttattgattg tcaccttgtt cctgctgacc
    15781 ttcgttggct tcagcttgag ctatttcact ccacacgcgc cgttacaagg cgcgtcactg
    15841 tggaatgcct gggtgttctg gtttaatggc ctgatccact gggattttgg tgtttccagc
    15901 attaacggtc aaccaattgc cgaacagtta aaagagctct tcccggcgac gatagaactg
    15961 tgcatcctcg ccttcggttt tgcgctgatt gttggcatcc ccgtggggat gattgccggg
    16021 attacgcgcc ataagtggca ggataacttg atcaacgcca tcgccttact gggcttttca
    16081 atcccggtgt tctggctggc gcttctgttg acactgtttt gttcactcac gctaggttgg
    16141 ttgcccgttt cagggcgttt cgatctgctc tacgaagtga aaccgattac cggttttgcg
    16201 ttgattgatg cctggctttc ggactcacca tggcgggatg aaatgatcat gagcgcaatc
    16261 cgccatatga tattgcccgt gatcaccctg tcggttgcgc caacaactga agtgatccgc
    16321 ctgatgcgta tcagcactat tgaagtgtat gaccagaact atgtaaaagc ggcggcgacc
    16381 cgcggtttgt cgcgctttac tattttgcgt cgccacgttc tgcataacgc gttaccgccg
    16441 gttattcctc gcctgggctt acagttttcc accatgctga cgctggcgat gatcaccgaa
    16501 atggttttta gctggccggg tttagggcgc tggttaatta acgccatccg ccagcaggat
    16561 tacgcagcca tttccgccgg agtgatggtg tgtggctcac tggtcattat tgttaacgtg
    16621 atttctgata ttttgggtgc catggctaac cctctgaaac ataaggaatg gtatgcctta
    16681 cgatagcgta tacagcgaaa agcgcccgcc gggcacgctg cgtaccgcct ggcgcaaatt
    16741 ttatagtgac gcctctgcga tggtcggcct gtacggctgc gcgggactgg ctgtactgtg
    16801 tatttttggc ggctggtttg cgccttacgg catcgaccag caatttctcg gttatcaatt
    16861 attgccgcca tcatggtcgc gctatggcga agtttctttc ttcctgggga ctgacgacct
    16921 cggacgtgat gttttaagcc gtttactgag cggagctgcg ccaaccgtgg gtggcgcatt
    16981 tgtggtgacg cttgccgcga cgatctgtgg cctggtactg ggcaccttcg ccggggcgac
    17041 acacggcctc cgctcagcgg tgcttaacca tattctggat accttactgg cgatcccttc
    17101 gctgctgttg gcaattatcg ttgtagcgtt tgccggaccg agtttgtccc acgctatgtt
    17161 tgctgtctgg ctggcattgc tgccgcgtat ggtacgttcg atttacagca tggtgcatga
    17221 cgaactggaa aaagagtacg ttatcgccgc ccgtctggat ggcgcatcaa cgctgaatat
    17281 tctctggttt gctgtgatgc caaacatcac cgccgggctg gtcaccgaga tcacccgcgc
    17341 actgtcgatg gcaattctcg atatcgccgc gctgggcttt ctcgatctcg gcgcacaact
    17401 cccctcgcct gaatggggag cgatgctcgg tgatgcgctg gaactgattt atgtcgcccc
    17461 gtggactgtc atgctgccag gtgcggcaat tatgattagc gtgttgttgg ttaacctcct
    17521 tggcgatggc gttcgtcgtg cgattattgc gggggtggaa taatgccatt actcgatatt
    17581 cgtaacctga ccattgaatt taaaaccggt gatgagtggg ttaaagccgt cgaccgggta
    17641 agtatgacct taaccgaagg tgaaatccgc ggtcttgttg gtgaatccgg ttccggcaag
    17701 agtttgattg cgaaagcgat ttgtggggtg aataaagata actggcgtgt tactgctgac
    17761 cgtatgcgtt ttgatgatat cgatttgctg cgtctctccg cacgcgaacg gcgcaaactg
    17821 gttggccata acgtgtcgat gattttccag gaaccgcagt cgtgtcttga cccttcagaa
    17881 cgtgtgggtc gccagttgat gcaaaacatc ccagcctgga cctataaagg ccgttggtgg
    17941 cagcgttttg gctggcgcaa acgccgtgcc attgaactgc tgcaccgcgt ggggattaaa
    18001 gatcacaaag atgcgatgcg cagttttccc tatgagttga ccgaaggtga atgtcagaaa
    18061 gtgatgatag ccattgcact ggcgaatcaa ccgcgtctgc tgattgctga cgaaccgaca
    18121 aactcaatgg agccaacaac ccaggcgcaa atctttcgcc tgctgacgcg tctcaaccaa
    18181 aacagcaata ccactatttt gcttatcagc catgacttac aaatgcttag ccaatgggcg
    18241 gataaaatta acgtgcttta ctgcggtcaa acagtggaaa ccgcgccgag taaggagttg
    18301 gtgacgatgc cacatcatcc ttatacccag gcgctgatcc gcgcgatacc agacttcggc
    18361 agcgcgatgc cgcataaaag tcgcctcaat actctacccg gcgctatccc attgctggaa
    18421 cagttaccga ttggttgtcg tctggggcca cgttgcccgt atgcacaacg agaatgcatt
    18481 gtgacgccac gtttgacggg ggcgaaaaat catctctatg cctgtcattt cccgctgaac
    18541 atggagaaag agtgagatga tcgaaacgct gcttgaagtg cgtaatttaa gtaaaacgtt
    18601 ccgctaccgg accggttggt ttcgtcgtca gaccgtagaa gcggtaaaac ccttgagctt
    18661 tacgctacgt gaaggccaga cactggcgat tattggcgag aatggttcgg gtaaatccac
    18721 actggcaaaa atgctggcgg gaatgataga acctaccagc ggcgagttat tgattgacga
    18781 tcatccgctg cattttggcg attattcctt ccgtagtcag cgtattcgca tgatttttca
    18841 ggatccttcg acctcgttga atccccgtca acgtatctca caaattctgg attttccact
    18901 gcgcctgaac accgacctgg aaccggaaca gcgtcgcaaa cagattattg aaacgatgcg
    18961 tatggttggg ctgctaccgg atcacgtcag ttactatccg catatgttgg cacccggaca
    19021 aaaacagcgt ctgggtctgg cgcgcgcgct gatattgcgc ccaaaagtca ttattgccga
    19081 tgaagccctc gcttcactgg atatgtcaat gcgttcgcag ttgattaatc tgatgctgga
    19141 gttacaggaa aaacagggca tttcgtatat ttatgttacc cagcatatcg gaatgatgaa
    19201 gcacattagc gaccaggtgc tggtgatgca tcagggcgag gttgtcgagc gaggcagcac
    19261 cgcggatgtg ctggcatcgc cgctgcatga actcaccaaa cggctgattg ccgggcactt
    19321 tggcgaagca ttgacagcgg atgcgtggcg taaagatcgc taattttctg aatcctcctt
    19381 ccagattatt cacctcattc tgacaagaga ttaaatcttg tcagaatgag gtgatggata
    19441 aaataaaatc aaatgcgcgt gatttaagac gcaatctcac tttgcaggaa cgaaagctct
    19501 ggcgatatct tcgcagccga cgttttggtg atttcaaatt tcgccgtcaa catccggtgg
    19561 ggagctacat tctcgatttt gcttgctgct cggcgcgcgt agtcgttgag ctggatggtg
    19621 ggcagcatga tttggcagtt gcctatgata ccaggcgcac tagctggctt gagtcgcagg
    19681 gctggaccgt gctgcgtttc tggaataacg agattgattg taatgaggag gcggtgctgg
    19741 agattattct gcaggaactg aaccgccggt caccctcacc ctgaaagggc gagggggccg
    19801 accgagccga atagctgttg tggtgaaaac atggagacag tgctggagaa tattcggcaa
    19861 ggtctgaacc atcccagcca tcgccataaa agggttaggg gctgtatgag cctgtttgtt
    19921 gctggggtaa caatatttgc acaatatggt cccctcgccc ctctggggag agggttaggg
    19981 taaggggaaa agcgcccccc ctgccgcagc ctgctccggt cggacctggt aactatagct
    20041 actcacagcc aggttgatta taataaccgt ttatctgttc gtactgttta ctaaaacgac
    20101 gaatcgcctg attttcaggc acaacaagca tcaacaataa ggattaaagc tatgggtttt
    20161 ctttccggta agcgcattct ggtaaccggt gttgccagca aactgtccat cgcctacggt
    20221 atcgctcagg cgatgcaccg cgaaggagct gaactggcat tcacctacca gaacgacaaa
    20281 ctgaaaggcc gcgtagaaga atttgccgct caattgggtt ctgacatcgt tctgcagtgc
    20341 gatgttgcag aagatgccag catcgacacc atgttcgctg aactggggaa agtttggccg
    20401 aaatttgacg gtttcgtaca ctctattggt tttgcacctg gcgatcagct ggatggtgac
    20461 tatgttaacg ccgttacccg tgaaagcttc aaaattgccc acgacatcag ctcctacagc
    20521 ttcgttgcaa tggcaaaagc ttgccgctcc atgctgaatc cgggttctgc cctgctgacc
    20581 ctttcctacc ttggcgctga gcgcgctatc ccgaactaca acgttatggg tctggcaaaa
    20641 gcgtctctgg aagcgaacgt gcgctatatg gcgaacgcga tgggtccgga aggtgtgcgt
    20701 gttaacgcca tctctgctgg tccgatccgt actctggcgg cctccggtat caaagacttc
    20761 cgcaaaatgc tggctcattg cgaagccgtt accccgattc gccgtacagt tactattgaa
    20821 gatgtgggta actctgcggc attcctgtgc tccgatctct ctgccggtat ctccggtgaa
    20881 gtagtccacg ttgacggcgg tttcagcatc gctgcaatga acgaactcga actgaaataa
    20941 tcgttctgtt ggtaaagatg ggcggcgttc tgccgcccgt tatctctgtt atacctttct
    21001 gatatttgtt atcgccgatc cgtctttctc cccttcccgc cttgcgtcag gataacgatt
    21061 tcctttacga ccaaggagcg cccatggaac aacgccacat caccggcaaa agccactggt
    21121 atcatgaaac gcaatccagt actacggagt atgacgttct gcctctggtc ccggaagccg
    21181 caaaggtcag cgatcccttt ctgctcgacg tgatccttga tgaagagacg ctggccccct
    21241 tcctttcatg gctggtccct gcgcgcgttc ttgcagtgga gttgttccct gaccagctta
    21301 ccgtgacccg ttcacaaacc ttcaccgctt atgaacgctt gtcgacggcc ctgacggttg
    21361 ctcaggtttg cggcgtccag cggttatgta actactattc ggcgcgactt acgccgctcc
    21421 ccggacctga ttcctccagg gaaagtaatc atcggttggc acaaatcacg caatatgccc
    21481 gccaactggc tagctcgcct tctattatcg acaaccgatc gcgccagcat ctgaatgacg
    21541 tcggtcttac tgtctgggac tgtgtgatca ttaaccaaat cattggtttt attggctttc
    21601 aggcgcggac aattgcgaca tttcaggctt atctcgggca tccggtacgc tggttacccg
    21661 ggctggcgat acaaaactac gccgacgcgt cactgtttgc tgatgaatca atacgctggc
    21721 gaagcagcta tgaggtggaa aaactacctg aagagcacac aaaaagttca actgcagaac
    21781 tttgccaact ggccgaaaca ctctctctcc accctatttc actttccctt ctcgaaaagt
    21841 tgttaaacag cacacgggtc aatgcacagc cggataatca gcttgcggcg ttgttatgcg
    21901 cgcgtataaa tggcagtcct gcttgttttt ccacctgtat ggattcatca aatgaatata
    21961 aaaaaatcag cacccttctg cgcaagggcg aaaatgaaat taaccgatgg gctgaccgtc
    22021 attctgttga gcgcgctacc gttcaggcga tacaatggct gacccgagca cccgatcgct
    22081 ttagcgccgc ccagttcagc cctttactcg aacacgaaaa atcatcaacg cagattatta
    22141 atctgctggt atggagcggg ctgtgtggct ggataaatcg cttaaaaatc gcgttgggtg
    22201 agacatatta accttgccgc gtcagacaga ttcgcgtaaa actgtcagcc gctctaatgg
    22261 ccaccaaaat agacaattat gtttcaggac aacccgctgc tagcgcagct taaacagcaa
    22321 ctgcattccc agacgccacg cgctgaaggg gtggtaaaag ccacagaaaa aggctttggc
    22381 ttcctggaag tcgacgcaca aaaaagttat ttcattccgc cgccgcagat gaaaaaagtc
    22441 atgcatggcg accgaattat cgcggtgatc cacagtgaaa aagaacgtga atccgcagag
    22501 ccagaagaac tggttgaacc gttcctgact cgtttcgtgg gtaaagttca gggcaaaaat
    22561 gaccgtctga ccatcgttcc tgatcatcca cttttaaaag acgccattcc ttgccgcgca
    22621 gcccgtggcc tgaaccacga gtttaaagaa ggcgactggg cggttgccga aatgcgccgt
    22681 catccgctga aaggcgatcg ttctttctat gctgaactga cacaatacat cacttttggt
    22741 gacgaccact ttgtaccgtg gtgggttacc cttgcgcgcc ataatctgga aaaagaagca
    22801 ccagacggcg tcgctaccga aatgctcgat gaaggtctgg ttcgtgaaga tctgacctcg
    22861 ctggattttg tcaccatcga cagtgccagc actgaagata tggatgacgc ccttttcgct
    22921 aaggcgctgc cggatgacaa acttcagctg attgtggcga ttgccgatcc aaccgcgtgg
    22981 attgctgaag gcagcaagct ggacaaagcc gcgaaaattc gcgcattcac caactatctg
    23041 cctggcttca acatccctat gctgcctcgc gagctttctg acgatctctg ctcactgcgc
    23101 gccaatgaag tccgcccggt actggcatgc cgcatgacgc tctccgctga tggcaccatt
    23161 gaagataata tcgaattctt tgccgccacc atcgaatcca aagcgaagct ggtgtatgac
    23221 caggtttctg actggctgga aaataccggt gactggaagc ctgaaagtga agcaattgcc
    23281 gaacaagtcc gtttgctagc gcaaatttgc caacgccgcg gcgagtggcg tcataaccac
    23341 gcactggtgt ttaaagatcg cccggattac cgctttattc tcggtgaaaa aggtgaagtg
    23401 ctggatatcg tcgccgagcc tcgtcgcatt gccaaccgta tcgtcgaaga agcgatgatt
    23461 gccgctaaca tttgtgcggc ccgcgtactg cgcgataagc tcggttttgg catctataac
    23521 gtgcatatgg gctttgatcc ggcgaatgcc gacgcgctgg cagcgttgct gaaaacgcac
    23581 ggtctgcatg tcgatgccga agaagtgctc acgctggacg gtttctgcaa actgcgtcgt
    23641 gaactggatg cgcaaccaac tggtttcctc gacagccgca ttcgtcgctt ccagtcattt
    23701 gctgaaatta gctctgaacc cggtcctcac tttggcctcg gtctggaagc atacgccacc
    23761 tggacttcgc cgatccgtaa atatggcgac atgatcaacc accgtctgct gaaagcggtt
    23821 atcaaaggcg aaactgcgac gcgtccgcag gatgaaatca ctgtccaaat ggccgaacgt
    23881 cgccgtctca accggatggc agaacgtgat gttggcgact ggttatacgc acgcttcctg
    23941 aaagataaag ccgggaccgg cacccgtttc gcggcggaaa ttgtcgatat cagccgtggc
    24001 ggcatgcgtg tgcgtttggt tgataacggc gctatcgcct ttattccggc acctttctta
    24061 cacgctgtgc gcgatgaact ggtttgcagc caggaaaacg gcactgtaca aattaaaggt
    24121 gaaacggttt acaaagtaac tgacgttatt gacgtcacca ttgccgaagt ccgcatggaa
    24181 acccgcagca ttattgcgcg cccggtcgcg taatctcctt tcacggccca ttcctcatgg
    24241 atgggccgtt tatttccccg ctctaccttc gttatatcca ggcagatttt ttaactgact
    24301 ttcgtttgaa aactggcgtt tttccaaaac tggattacaa ataaatatat aaaaaaacaa
    24361 tgcgtttagc cgccgttaat ttcatgtaag tcatgttgtt ccttttattt aaccgcggac
    24421 tccgctgtta accggaggat atgcatcatg aaaaccgtta gggagtccac aacgttgtac
    24481 aactttctcg gatcgcacaa tccatactgg cggttgacgg aaagcagcga tgctttgcgc
    24541 ttttctacca ccaaaaccac agaacctgat cgtacattgc agttatctgc cgaacaggct
    24601 gctcgcatca gggaaatgac ggtcatcacc tccagcctga tgatgagtct gaccgtcgat
    24661 gaaagcgatc tttctgtgca tctggtagga cgaaaaatca ataaacggga atgggctggc
    24721 aacgcgtctg catggcatga cacaccggcg gttgctcgtg atttatcaca cgggctttcc
    24781 tttgctgagc aggtagtttc tgaagcacat tccgcaatag tgattctcga cagccggggg
    24841 aatatccaac gcttcaatcg gttatgtgaa gattacacag ggttgaaaga acacgacgtc
    24901 attgggtaaa gcgtgtttaa actgtttatg agccgtcgtg aagctgcggc atccaggcgc
    24961 aataaccgtg tattttttcg aagcggcaat gcatatgaag tcgaactgtg gataccaaca
    25021 cgtaaaggcc agcggctgtt tctgtttcgc aataaatttg tccacagcgg cagtggcaaa
    25081 aacgagattt ttttaatctg ttccggtacc gacattaccg aagagcgccg cactcaggag
    25141 cgactgcgta ttctggcaaa taccgacagt atcaccggac tgccgaatcg taacgcaatg
    25201 caggagttaa tcgatcacgc tattaatcag gcagataaca ataaagttgg ggttgtgtat
    25261 cttgatttgg ataattttaa aaaggtcaac gacgcctatg ggcatttgtt tggtgaccag
    25321 ttattacgcg acgtgtcatt ggctatttta agctgtctcg aacatgacca ggtgttggcg
    25381 cgtccaggtg gcgatgagtt tctggtactg gcatccaata cctcacaaag cgcgctggaa
    25441 gcaatggcat cacgaatttt gacccgctta cgcctcccct ttcgtattgg tttaattgaa
    25501 gtttatacca gctgttcagt aggtatttcg ctctctcccg aacatggttc ggacagcgcg
    25561 actattattc gtcacgccga cacagcaatg tacacagcga aggaaggcgg acgaggacaa
    25621 ttttgcgttt ttactccaga aatgaatcag cgggtatttg aatatctctg gctggatacc
    25681 aacttgcgta aagcactgga aaacgatcag ttggttatcg aaaatcacct agcgtgtcga
    25741 agtgcgcagt ctggaagcac tagtacgttg gcagtcacct gaacgtgggt tgattccacc
    25801 gttggacttc atttcctacg ccgaagagtc agggctaatt gtgcctttag gccgttgggt
    25861 gattctcgat gtcgtacgcc aggtggcaaa gtggcgggat aaaggcataa acctgcgagt
    25921 ggcggtaaat atttctgcac gtcagctcgc cgatcaaacc attttcaccg ccctgaaaca
    25981 ggttctccag gaactcaatt ttgaatactg ccctatagat gttgaactga cagagagttg
    26041 tctgattgag aatgatgaac tggcactgtc tgttattcaa caatttagcc aactaggtgc
    26101 gcaagtgcat ctggatgatt ttggtaccgg ctactcttca ctttcgcaac tggcgcgctt
    26161 tccgatcgat gccatcaaac ttgaccaggt ttttgttcga gatattcaca aacaacctgt
    26221 ctcgcagtca ctggtccggg cgatcgtcgc tgtggcccag gcattgaatc ttcaggtgat
    26281 cgccgaaggt gtagagagtg caaaggaaga tgctttttta accaagaacg ggatcaatga
    26341 gcggcaagga tttttgtttg ccaaaccgat gcccgccgtc gccttcgaac gctggtataa
    26401 acgctatctg aagcgcgcat aagtagatga atatatatac ttagcgctat atcccgtcat
    26461 tatatatcgt attcctggat gataattagc cgttgctgtt aaacacttct cttttagcgg
    26521 caatttttgc attattcatt ttcccccttt tgctataagg atgtccttga tgtctgaatt
    26581 tgatgcccag cgcgttgccg aacgtattga tattgtgtta gatatccttg tggcaggcga
    26641 ttaccactct gcgatccata atctcgagat acttaaagca gaactcttgc gccaggttgc
    26701 tgaatctacg ccagacatcc cgaagacacc gtgggagatt taacaatcgt gccttcaggt
    26761 tttcctattt gaggatgccc ggcgcgtcag tattttttta tttagtatta taacgttata
    26821 agaattacag cgatgaattc ccgacaacaa actattctac agatggtcat tgaccagggt
    26881 caggttagcg taaccgatct ggcaaaagcc actggagttt ctgaagttac cattcgccag
    26941 gatctcaaca ccctcgaaaa actgagttac ctccgccgtg cacatggctt tgcagtttcg
    27001 cttgatagtg atgacgtcga aacccgtatg atgagcaact atacgctgaa gcgtgaactc
    27061 gccgagtttg ccgcgtcact ggttcaaccg ggcgaaacca tctttatcga aaatggcagc
    27121 agcaatgccc tgctcgcccg gactctgggg gagcagaaga aaaatgtcac tatcatcacg
    27181 gtcagcagct acatcgcgca tttgctgaaa gacgcgcctt gtgaagtcat tttgctcggt
    27241 ggcgtgtacc agaaaaaaag cgaaagtatg gttggccctt tgacacgcca gtgcatccag
    27301 caggtacatt tcagcaaagc atttattggt attgatggct ggcaacctga aactggattc
    27361 accggtcgcg atatgatgcg caccgatgtg gtcaatgccg tgctggaaaa agagtgcgaa
    27421 gcgatagtcc tgactgacag ctcgaaattt ggtgctgtac attcatactc catcggtccc
    27481 gttgagcgat tcaatcgcgt gattaccgat tcgaaaatac gcgccagcga tctgatgcat
    27541 cttgagcaca gtaaactcac cgttcacgtc gttgacattt aaccatttcc cttctctacg
    27601 gatgatttgc agtttggcaa atcatccgct ctaagatgat tcctggttga taattaagac
    27661 tatttacctg ttattaacac tctcaagata taaaattatt atcagcgata taacaggaag
    27721 taattatcac ctgcgtgata taaccctgcg cgcgagcaga tttcacggaa taatttcacc
    27781 aggcttattc ttagctatta tagttataga gagcttactt ccgtgaatca taaattcagg
    27841 agagagtatt atgtttgtaa cgagcaaaaa aatgaccgcg gctgttctgg caattacttt
    27901 ggcaatgtct ctgagtgcct gttctaactg gtctaaacgg gaccgcaaca ccgcaatcgg
    27961 cgcgggtgca ggggcattag gcggtgcagt actgaccgat ggcagtacgt tgggtacatt
    28021 aggtggtgca gctgtcggtg gtgttattgg tcaccaggtc ggtaaataat cgtaaataaa
    28081 tgtcgattat cgactcgcaa ttataatacg cttgattctg ttcagtacga aataactgaa
    28141 taatacacac agtaaaatat tcgaaagcca cggatatatc cgtggctttt tatgttaacc
    28201 gcctgcgagt tttaccttca tccctttcgc ttcaagtagt gattttaata aatcacgctt
    28261 atcgccctgg atttcaataa ctccatcttt aactgctccg ccgcagccgc attttttctt
    28321 cagttccgct gcaagttttg tcagttcggc atcatcgaga tcgataccgg taatcaggca
    28381 aacgccctta cctttacgtc cactggtctg acgctgaata cgcaccgctc catcgccttt
    28441 agggcgtact ggggccgctt tgggctcatc aatacgcccg gtttccgttg agtagaccag
    28501 acggctgttg gaatcactca tcatgcactc cgctgtaaag aggcgttgat cgctttcagt
    28561 gtctgcgctg gatctaccga ttgcgttacc gggcgaccaa tcaccatata atcaacacca
    28621 gccgccaacg cctgttctgg cgtcataatg cggcgctggt caccagcttc actcccctgc
    28681 ggacgaatgc ccggtgtaac cagtttgaac tcctgaccga atacctgttt aaagcgcaca
    28741 gcttcctgag cagaacacac cacaccatca aggtcacatt tttgcgtcag tgctgccaga
    28801 cgttctgcat aatctgcagg tgacagtgtc acgccaagat cggccaggtc gctggcttcc
    28861 atgctggtca acactgtcac agcaatcaaa agcggtgcat ctttgccaaa cggaaccagt
    28921 gcctcacgcg ctgcggtcat catacgcgcc ccaccggagg catgaacatt caccatccac
    28981 acgcctaagt cagctgcagc agcgacagcg tgcgctgcag tgttggggat atcgtggaat
    29041 ttcaggtcaa gaaagatatc aaaaccacgc tgttgaagtt cgcgcacaaa ctgtggccca
    29101 aacaatgtaa acatctcttt accgaccttc agacgacaat cgcgtgggtc gatcttgtcg
    29161 acaaaggaca gcgcgtcatc acaattatga taatcaaggg caacaaccac aggagaattc
    29221 gtaacggcgc gggaagaaga tgaagcagtt aacgtcatga ccagaccttc ttgatgatgg
    29281 gcaccgggag tggcgcgaaa caggtaaacg gcgagcattc tacccgtgaa aataaaaaat
    29341 taacagttgc gatttttccc ctagcaggct ttttctgctc cgtcgttgcc ggagcacaat
    29401 gggacatcaa taactagtat gttgtaacta aagtcggatt tttttaaaaa ttacaggcca
    29461 tcaagaccgc gaatcggttt aatagttgac caggcacgac aagacggaca atgccagtag
    29521 agggtgtatg cggtaaaacc acatttctgg cagctataac gaggcttact acgcaccttc
    29581 tcgccaacca tgtcacgcag caccatcaga ctctctttgg cgcgcccttc ttccgcttca
    29641 tttaagtggt aatccattag cttatggaat acacgcatgg tcggatgacg ctgaagctgg
    29701 cgtgtaatat agacctgtgc agcctcacta ccgtcgcgcg cttcgatgat atcagcaagc
    29761 atcaattcag catcggcacc ggtgttctct tccaccgcgc gttgcaggaa ttctgcccat
    29821 tcggcagttt tacccaactg ctgatagcag gtttgcaaca tttccagcgt ttcgctgacc
    29881 agttctctgt cctgggatat gacgcgttgc aaactttcga cggctttggc gtattctcct
    29941 ttcgccataa acacgcgtcc catcatgatg gatacgcggg cgctgttttt atctgccgcc
    30001 gccccttttt ttagcaaggt catggcacga tcgagatcgt cgctggccat atgctgcagg
    30061 gctaactcac agtagaaatg ggcaatttcg acgcgctgtt tatctttacc cagcttcacc
    30121 aggcgttcgg caacatcaat tgctttctgc cactcactgg tagcctggta gatttgtagc
    30181 aactgttgca gcgcgccaat gcggaagtca gtttcatcgg tcagctgatt gaacatgtct
    30241 tccgcgcggt cgtataaccc ggcggccatg taatcacgcc ccagttgttg aatcgccaac
    30301 agacgctgtt cataggtcag cgaggcgctt tccattaggg tctgatggat gcgaatagcg
    30361 cgatcaactt cgccacgcga acggaacagg tttccgagcg taaggtgggc ttcaacggtg
    30421 cctgtatcct ctttaagcat atcgagaaac aggtctaccg ctttatcctg ttgattacta
    30481 agcaggaagt taacccccgc tacgtaatca cgcgacaagc ggttggcttc atcttgcttg
    30541 ttttgttgcg cacttctgcg gcccatatac cagccatagg cagcggctac aggcaaaagc
    30601 agaaacaaca actccagcat agaaagttat tccttcgccg ctgacgagtg cggcactaca
    30661 gccacgtcag tcgcgggtga aagctggttt tccagtcgct ttattttacg ttcagcgcgc
    30721 gccagggaaa cacgaactcg cagccagaac aggccacaaa tcaaccaacc gatagcaaac
    30781 cccgcagcaa acaataccgc cagcaatgtg gaaatacggt actccccttg cgctaacaga
    30841 taattaaacg tcacctgttg atcgttctgc gcacccaacg tgaccgaaat cacgaagatc
    30901 gctaacacca gtaagaaaat gagtaaatat ttcacattac ttcccgttat gtggttagag
    30961 cgaataaatt atggtcaact taccgcaata gccctttaaa gtaccatttt cgcgccgtct
    31021 gcgaaatggc atccacgcgc attgttgatg atttaaggcc taaattcgga aaatcaacca
    31081 gcgttaactt tcttgttctc gttgtgcaat ttcgcgattt tcttccgcag gtggtgttaa
    31141 tggcccacaa attcgttgcg caagccacgt tgccaccgcc accagcgccc acgaaatcaa
    31201 cgtagctacc accagatcgc gtggccaatg catcccgagc agcaggcggc ttcccatgac
    31261 tcccgttgcc cagaccaaca agatagcaat ggttaacgtt cgccgacgcg gccacagcaa
    31321 accaacggcc agcagtgccc aactggcagc aaacatcgtg tgaccggaag gaaaggcaaa
    31381 ccccgtctct ttctgccagt gtgaacgcaa atattgtgga atatttttct cttcagccaa
    31441 ctgttctttt actagatttc cgcgttctgc tcgctttaaa gtgtagaact catcaaccgg
    31501 aatatgatgt gttttttcca accagataac aaaaggtcgt ggttcctgta ctttgtcttt
    31561 gatccaggat ttaacgcctt gtccaacaag gattgccgcc gccagaatgg caaataatac
    31621 aaaggcagcc ttaatgcgaa aacgcagaca ccagagaaac cagccgaaca aaatcaaatg
    31681 tgtaatgacg ccccagggct gggtgacagt ttcagtaacc caaaaagccg cttttagtag
    31741 ccaactttgt tctccaggtt gccaacgcca gccagaaatc catacggcta ctggcatgac
    31801 aagcaatagt gcagctccca ctgcggtacg tcgggcaatc gaacgcatgg cctctccttt
    31861 ttgataagtc ccacaatcat aactgaaaat gccagttcca agaaaaattg acagatttgt
    31921 gccattccgt gaacgatcga cgcgtcgtga ttaggtgaac cccttctcgt tatggcaaaa
    31981 taagccaata cagaaccagc attatctgga gaatttcatg cagcttaaac gtgtggcaga
    32041 agccaaactg ccaaccccat ggggcgattt cctgatggtg ggatttgaag aactggcaac
    32101 cggacacgat catgttgcgc tagtctatgg cgatatttcc gggcataccc cggtacttgc
    32161 gcgcgtccat tccgaatgtc tgaccggtga cgccctgttc agcttgcgct gcgattgtgg
    32221 cttccagctc gaagcggcat tgacgcaaat tgccgaggaa ggccgtggta ttttgctgta
    32281 tcaccgtcag gaaggtcgta acattggtct gctgaataaa atccgcgctt acgcactgca
    32341 ggatcaaggt tacgataccg tagaggctaa ccaccagtta ggcttcgccg ctgatgagcg
    32401 cgacttcact ctttgcgctg atatgttcaa actccttggc gtcaatgaag tccgcttgtt
    32461 aaccaataac ccgaaaaaag tcgaaattct gaccgaagca gggattaata ttgttgaacg
    32521 cgtaccattg attgtaggtc gtaaccccaa taacgaacat tatctcgata ccaaagccga
    32581 gaaaatgggc catttgctga acaaataacc ctcttgcatc gtgtaattca tttgcttgcc
    32641 ggaagcaaaa taaccggcaa gcaaatactt gttacttcaa catattacga atgacataat
    32701 gcaaaatgcc gtcgttctgg tagtaggtca actccgtcgc ggtgtcgata cgacaacggc
    32761 agggtacgac ttcctggcta ccatccgcgc gcgtaagcgt caccggaacc gtcgcgccag
    32821 gttgtagatt ttgcagatcg ccaatatcaa tcttctcttc cccggttagc cctaacgttt
    32881 tacgcgtcac gccttgcgga aattccagcg gcaggatgcc catgccaatt aaattcgaac
    32941 ggtgaattcg ttcaaacgat tcggcaatca ccacacgaat tccaagcaga cgaggtcctt
    33001 ttgccgccca gtcacggctt gagcctgatc catactcttt cccggcaatc accgccagcg
    33061 gcgtttgctc ctgcttatag cgcatcgcag catcataaat agagactacg tcgctgtcag
    33121 gtaaatgccg cgtcatcccc ccttcaacgc caggcaccat ttcattacgg atgcgaatat
    33181 tggcgaaggt gccgcgcatc atcacttcat ggttaccacg ccgcgaaccg taggagttaa
    33241 agtcttttcg ctcaacaccc cgaccttgta gatatcgacc cgctgggctg tcgggcttaa
    33301 tactgcccgc cggagagata tggtcagtgg tgactgaatc ccccagcatt gcgaggatcc
    33361 gcgcaccgtg aatatcttcc actggtgctg gtgttgcctg catttcatca aagaaaggcg
    33421 ataagcgaat ataggttgag tcctcctgcc aaccgtaggt atcggatcgt gtgacgttaa
    33481 tttccttcca ctctgctgtg ccttcaaaaa cttctgcgta ctctttgcgg aacatttctg
    33541 tggagacttg ttctaccgca cgggcaattt cttgtgccga tggccagata tctttcagat
    33601 aaaccggctc gcctttgcga tcatggccga taggctcaga agccaggttg atattcatat
    33661 ttcccgccag cgcataggca accaccagcg gtggcgaggc cagccagtta gttttaacca
    33721 gcggatggat acggccttca aagttacggt tgccggacag caccgcaccg acggttaaat
    33781 cgcctttttt gattgccgtt tcgataggat cgggcagcgg cccagagtta ccaatacagg
    33841 tggtacaacc gtatcccaca aggttaaacc ccagttcgtc gagatacggt gtcagtttcg
    33901 cttttgccag ataatcagaa acgactttcg aacccggtgc caacgacgct ttgacccacg
    33961 gttgccgctt gaggccaaga gttacggctt ttttcgccag caagcctgcg gccatcagca
    34021 cacttgggtt agaggtgttg gtgcacgagg ttatcgcagc aatgaccaca gcgccatcag
    34081 gtaactgata ctgatgtccg ttcataacat aatcgactgg caggcgatct ttatgcgtgg
    34141 cattcacttt cagttcgtta ctggcggcaa atgcttttgg tacatcgggc agtgcaacgc
    34201 gatcctgtgg gcgttttggc cctgccaggc tcgcttcaac gtcattcata tccagttcta
    34261 acatactggt aaaaatcggt tcatcgcccg ggttacgcca catgccctgc gctttggcat
    34321 atttttcgac caactcgacc tgatcttcgc tgcgcccgct taaacgcatg taatcgaggg
    34381 ttactgcatc gattgggaag aagccacagg tggcaccata ttctggcgac atattggcaa
    34441 tggtggcgcg atccgccaac ggtagtgaat ccagaccatc accataaaat tcgacgaatt
    34501 tccccaccac gccatgtttg cgcagcattt gggtaacagt gagaaccagg tctgtggcgg
    34561 taataccttc acgtaatttt ccggtaagtt taaagcccac tacgtccggg ataagcatgg
    34621 aaaccggctg gcctaacatg gcggcttctg cttcgatccc acccacgccc caccccagca
    34681 cgccaaggcc gttgatcata gtggtgtgcg agtcagtacc aacgagtgta tccggataag
    34741 caatccattc accgtcctgc aattcgctcc acactgcttt gccgagatat tcgaggttaa
    34801 cctgatggca aatgcctgtg cctggcggca cgacgctaaa ccgactgaac gcttgctttc
    34861 cccatttcag gaacacataa cgttcgtggt tgcgctccat ttccaggcgt acgttttctt
    34921 caaatgcctc atcatcacca aaacgatcga cggtcaccga gtggtcaatg accaggtcga
    34981 ccggtgagag cgggttaacc tttgcagtat cgccaccgag gcgtttaacc gcttcgcgca
    35041 ttgccgccag atcaacaacg gcaggtacgc cggtaaagtc ctgcatcagc acccttgccg
    35101 ggcggtaggc aatttcacgg tcagcatggg catttttcag ccatcccgcc agtgcgtgga
    35161 tatcctcttc ggtaactgag ttaccatcct gccagcgcag caggttttcg agcaaaactt
    35221 tgagtgactt gggtagacgg gtgatatcgc ccagtgattt agcagcaagc ggcaggctgt
    35281 agtagtggta agttttatct ttggcctgca acgtgtcctt actggcttct cgtagggttg
    35341 acgacatagc tcctccttaa tgacagggtt gcgaagccct gattctcttc agggtgaata
    35401 ttaaagataa cacaaacatc gcgtaacgat ttgataacaa cccaaattga taaaagagga
    35461 gaaaccttgt ggcaaagcaa atgacaaccc cgccgcagcg gggtcaagga tcagtgagta
    35521 atgatccaga ggagttcggc ccagaagagc atcgacagag aaaacgcgcc catccaggac
    35581 caatatttca tatttgtgtt gatcataggc tacaccctta acggttatta ttttgtgttg
    35641 agataactca agctgttatt gctgcggtgt attaaaagcc ctttaattca ttaaaaatgg
    35701 gtaataagaa tgatgataat tattgtttcg gctgatcatc ttcagcaaac agttctataa
    35761 aggcttgctg ctgcgatgtt agttgccagt cgtctggaat aacgacggaa cattcccgtt
    35821 tcgtttctgg ttcattgtca ctatgctggc ataatggtga cgactccagt tgctcctgac
    35881 cgaccatagt tatccccaca ctttccaaat cagcagtgcg acaaccaccc agaaaagtgc
    35941 agaaccaaga aaaaccgcca gccaggcttt acgttttaat tcaggatcac ggcggggttc
    36001 ctgatttcca gaaggcattg ctaacctcat ataatcgcca tcacttatca gcaagacgtt
    36061 gaatgattgg tgtaattaat catacgatga gacaaatcct aaagaaactt tagtcaaaaa
    36121 tccagcgaat ttacgaatcg tctccagtga ataattcaat cttttgacct gaaatagata
    36181 tttgaaaact ggaatttgcg aggcgggtaa ttagacacta ctttacaggt aatttaccct
    36241 ataaatatga gggataaaaa tattgtgcac ggtatagaaa taagtaacaa aagagaatat
    36301 attccggcac cttcgctaca taaaaggtgc cgaaaataac gcaagaaatt atttttccgg
    36361 cagttttata tctttaaaca tgacctcaat ttcttcattg gagcgcaatg cgacagccgc
    36421 atcaacgaca tcacgcgtta aatgcggtgc aaaacgctga atgaaatcat acatataact
    36481 gcgcaaaaaa gtactacggc gaaaaccaat tttggttgta ctgtggctga agatatcgtg
    36541 agcatcaacg cgcacaaggt cgggatcggc gaccggatcc accgccatgc tggcaatgac
    36601 ccctaccccc agccctaacc ggacgtaagt tttaatgacg tcagcatccg ttgccgtgaa
    36661 aacgatacgc ggcgttaacc ctgcgcggtt aaaggcagta tccagttctg aacgtccggt
    36721 aaagccgaag gtatatgtca ccaacggata ttgcgccagt tcttcaatgg taatggcttt
    36781 tttgcctgcc agcgggtgat ccggagtgac tacaatagcc cgattccagt ggtagcacgg
    36841 taacatcact aaatcttcat acagatgcag cgcttctgtg gcgatagcga aatcggcatt
    36901 gcctttagag acggcatcag caatttgtgt cggcgggccc tggtgcatat gcaaagaaac
    36961 gcggggataa cgctcaataa agcctttgat aacgtttggt aacgcgtagc gtgcctgggt
    37021 atgcgtggtg gcgatataca gcgagccttt atccggccag gtgtgctcac cggcaaccga
    37081 ttttatggca tcgactttcg acaggacttc gcgagcgata cgaattattt cttgtcctgc
    37141 tggcgttacc tgcgtcaggt gcttgccgct tcgggaaaaa atttgaatgc ctagctcgtc
    37201 ttccagcatt ctgacttgtt tactgatccc gggttgtgat gtgtaaagtc cttccgctgt
    37261 tgatgagaca ttcaggttat gattgaccac ctcaacaata tagcgaagtt gttgtaattt
    37321 catgttaaac catccactta gacgccatca gaacatcgtt ttttcgtcac acctggcggg
    37381 tgactaatac gatttaataa taaaaaaggt gctaactata accactatat catttatagc
    37441 cttagtgtat aggtgtgaag aaaaaataat atgcaataaa aaagggccgc tttcgcgacc
    37501 ctttgtttat aaaaacctga cagaattaaa ggttattttt ttccttcaac ccatttgcca
    37561 tcaacataaa atgctgacca gccagtcgcc tttccgtctt tttccgaaga gacatattgc
    37621 tgtttggttt tacggctaaa gcgaaccatg gtcttattac cttccggatc ctgctgtggc
    37681 gcatcggcca gataacgcag tttttccggc agacggtcgc ggaagcgata aagctcttcc
    37741 accagtggcg cacgcgtttc acgcgatttc gggaaagtgt tggcagccag gaacacaccg
    37801 gcagcaccgt cacgcagcac gaaataagca tctgattttt cgcacggcag ctcaggtaat
    37861 ggcaccggat cttctttcgg tggtgccact tcaccgttac gtaaaatctt acgtgtgttt
    37921 ttacactctt cgttggtgca ggccatgtat ttaccgaaac gccccatttt caggtgcatt
    37981 tcagagccac atttttcaca ctcaacgatc gggccgtcat aacctttaat gcggaattcg
    38041 ccctcttcga tctcgtaacc gtcgcaggtt gggttattac cacagacatg caacttacgt
    38101 ttcggatcga tgagatagct gtccatcgcc gtgccgcatt tcgggcaacg acgttttgcg
    38161 cgcagcgcgt tggtttcagc atcttcgcct tccagcacgt tcagcacttc gttttccggc
    38221 accaggttaa tggtggtttt gcaacgctct ttcggcggca gcgcatagcc agaacagcca
    38281 aggaataccc cggtactcgc tgtgcgaatc cccattttgc gaccacaagt cgggcagtca
    38341 atgctggtca gaaccatctg gttcgggcgc ataccaccct cttccggatc tttttcagct
    38401 ttatctaact gctgggtgaa atccgagaag aagtgatcca gtacagcttt ccactctgct
    38461 tcgtgatttg ccacctggtc gaggctgttt tccatctgcg cggtaaagtc gtagttcatt
    38521 aactcgcgga aattttcttc aaggcgatcg gtgacgattt cgcccatttt ttccgcatag
    38581 aaacgacgat tttctactcg cacatagcca cgatcctgaa tggtcgaaat gatcgacgca
    38641 taggtagacg gacgaccgat accacgtttt tccagttctt taaccagcga tgcttcactg
    38701 aaacgggctg gcggcttggt aaagtgctgg gctggtgtta gttcaacgag cgtcagagca
    38761 tcgcctttat cgactgccgg taagatacga tcttcatcgc ctttacgcag tgcaggcatc
    38821 acttttgtcc agccatcaaa gcgcaaaata cgaccgcgtg ctttcaggcg gaaatcgccc
    38881 gcaccaacgg tcagcgtcgt ggagtcatat ttcgctgggg tcatctggca ggcaacgaac
    38941 tgacgccaga ttaactggta cagtttctgc gcatctgctt ccatatcctt cagcgattcc
    39001 gccatcacat tgacgtcaga aggacgaatc gcttcgtgcg cttcctgtga gttttctttg
    39061 ctggcgtact gattcggact ttccggcaga tatttcttac caaaattatc gctgatataa
    39121 ccgcgaacca tatttaccgc gtcctgactc aggttagtgg agtcggtacg catgtaagtg
    39181 atatagcctg cttcatacaa acgctgcgcc atcatcatgg tttttttcac gccaaaacca
    39241 agacgggtgc tggcagcttg ttgcagcgta gaggtaataa aaggagcgcc aggtttactg
    39301 gttgtcggtt tgtcttcacg ttccagcacg ctgtagcgcg ctttttccag cagacttacc
    39361 gcagcctgag tttgttcttt gttgaccgga cggaacggtt tgtcattctg atgagtcacc
    39421 tgcaacgcca acgcttcacc agatggcgta gtcgtgctgg catcgacttc ccagaactct
    39481 tccggtacga acgctttaat ttcacgctca cgctctacca ccaggcggac cgccaccgac
    39541 tgcacacgac cggcagacag accacgagcg atctttttcc acagcagcgg cgaaaccata
    39601 taccccacca cgcggtccat aaagcgacgc gcctgctggg cattaacacg atcaatattc
    39661 agctcacccg gtttgttaaa tgcctggcgg atcgcgtttt tagtaatttc gttaaacacc
    39721 actcggctat agcgcgcatc atcaccacca atcacttccc gcaggtgcca tgcaatggct
    39781 tccccttcgc ggtcaaggtc ggttgcgaga tagatgtggt cggctttttc agccagttgt
    39841 ttcagttcag agacgacctt ctctttacca ggcaacactt catagtgcgc ctcccaattg
    39901 tgccacgggt caacccccat acggttgacg agagcgccac gttcatcctt tttaggcttt
    39961 ttagccgtct tggtggaggt agagtcggca ctctttttgg cagctgagcc actggtcggc
    40021 aaatcgcgga tgtgaccgac gctggatttc accacgtagt cacaacccag atacttgttg
    40081 atcgttttgg cttttgccgg ggactcaacg atgacaagag ctttacccat attcacctta
    40141 cctaatttga ttcttccagg aatgcgtcgc acgttgataa accttccact ggcaacgagt
    40201 taccgatatg gactcgctct ctgcggatat caaccccttg tcgtgactct gttcacgaaa
    40261 attcaggtga ctgagttaac aggtcttggc ctacaggcgc tatagcacga cggaattttg
    40321 gtcgaatgtc aagcaaatct attgccagat tgacgaaagc gtcacactgt acctgataaa
    40381 atccgatacg caactttatt agcatgcaaa atccaaagtt gccagtaagg cgctcttgcc
    40441 accagcgtgg tatatttgtc tgcaagccat gcctggcgta gactaatgtc cttgtttaag
    40501 gagtaaataa tgaacaaaga gacacaaccc atcgaccgcg aaacgctgtt gaaagaggcc
    40561 aacaaaatca ttcgtgagca tgaggatacg ctcgcaggga ttgaagccac cggcgtcacg
    40621 cagcgtaatg gcgtgctggt atttaccggc gactactttt tagatgaaca aggattgcca
    40681 acagccaaaa gcaccgcggt gtttaatatg ttcaaacacc tggcccatgt gctttcagaa
    40741 aaatatcatc tggttgatta aagcaaaacg cgaggtctta gcctcgcgtt tgtcttttac
    40801 atcaatggct tttgaccccg ctgccaccag cgtagcaaca atcgatcggc gctctctgcc
    40861 gcgctgccgg tgaatcggtc aatgagtcgt ttacgctgca tatagcgtac attaaccact
    40921 tcacggcctt ccatcaggct aagaataact tcatcactgg tgttgatttc atcaaccagg
    40981 cctttctcta ccgcctgttg tccgtaccag tgttcacccg ttgccacctg ttcaatatcc
    41041 agagacggac gcatacgctt cacaaaatct ttaaataact gatgcgtttc gttcagctct
    41101 tcgcggaatt tctcccgccc ttcttcggta ttttcaccca gcaaggtcag cgtgcgctta
    41161 tactgcccgg cggtgtgcag ttcgatatca atatctttgc ttttcaggaa gcggttaaag
    41221 ttgggcattt gcgccaccac cccaatggaa cccacaatag caaacggtgc ggaaacaatt
    41281 ttgtccgcca cacaggccat catgtaaccg ccgctggcag cgactttgtc taccgtaaca
    41341 gttaaaggaa tgtttttatc acgcagacgc tgcagctgcg aagccgccaa cccgtaacca
    41401 tgcaccatgc caccagggct ttccagacgg agcacaacct gatcctgcgg tttgaatgct
    41461 gcgagtaccg ccgttatctc ttcacgtagc gagttcactt catgggcgtc catgctgcct
    41521 ttaaaatcca gcacccagac gcgaggttta ctgtcagttg ccacctcgcc cagtttggct
    41581 ttcgcttttg ctgctttcgc ttcttgcttg tgctttttct tctgcgcttt gtgccactgt
    41641 ttttgctgat gtgagtccat cagcgccgcg gccagttctt ctttcatctc cttatactgt
    41701 tcgctgagat tgttgacccg taactcgcca cgctggcgtt tattacgttg agcaacattg
    41761 acaataatgg cggcaatcgc cgcaatcgct agcacaacgg taacgatttt agccaaaaac
    41821 aaaccatatt cagacaacaa ttccacgagt ccccaccttg gttaaacaac gctttggctc
    41881 acagtttaca acaggcatgt taagccgtct cgcagagatg ttattgcagc gaaagtatga
    41941 caaagtatcc attttgcggt gttatcgccc caggctgatt gaaatgccct tctgtttcag
    42001 gcataaagcc ccaaagtcat aaagtacact ggcagcgcga cagacaacgt cgttgaggag
    42061 tagccatgca ttaccagcca aaacaagatt tactcaatga tcgcattatc ctggtgacgg
    42121 gagccagcga tgggattggt cgtgaagccg cgatgacgta tgcacgctat ggtgcgacag
    42181 tgattctgtt gggccgtaat gaagaaaaat tacgtcaggt agccagccac ataaacgaag
    42241 aaactgggcg tcagccacag tggtttattc tcgatttgct gacctgcacg tccgaaaatt
    42301 gccaacaact ggcacagcgc attgtcgtta attatccgcg tctggatggt gttttgcata
    42361 atgccggatt gctcggcgat gtttgcccaa tgagcgaaca aaatccgcag gtctggcagg
    42421 acgtcatgca gatcaacgtt aatgccacct ttatgctcac ccaggcactg cttcctttat
    42481 tactcaaatc ggacgccggt tcactggtct ttacttcatc aagcgttgga cgtcagggac
    42541 gagccaactg gggtgcatat gcagcgtcga aatttgccac cgaagggatg atgcaggtac
    42601 tggccgatga atatcagcag cgcctgcgcg tcaactgcat taacccaggc ggtacgcgca
    42661 ccgcaatgcg cgcaagcgcc ttcccgaccg aagatccaca gaaacttaaa acacccgctg
    42721 atatcatgcc gctctacctc tggctgatgg gcgatgacag ccgccgtaaa accggcatga
    42781 cctttgacgc ccaaccgggc cgtaaaccag gaatttccca atgagtgatg aacgctacca
    42841 acagcgtcag cagcgagtga aagaaaaagt agatgctcgt gtggcccagg cccaggatga
    42901 acgcggtatt atcatcgtct ttaccggcaa tggaaaaggc aaaaccaccg cggcatttgg
    42961 tacggcaaca cgcgcagttg gtcacggaaa aaaagtgggc gtcgtgcagt ttattaaagg
    43021 cacctggcct aatggcgaac gcaatctgct ggagccacat ggcgttgagt ttcaggtgat
    43081 ggcaacgggc tttacctggg atacacaaaa ccgcgagtct gataccgccg cctgccgcga
    43141 agtctggcaa catgcaaagc ggatgcttgc tgattcctca ctggatatgg ttttgcttga
    43201 tgaactgacg tatatggtgg cgtatgacta tttgccactg gaagaagtgg tgcaggcgtt
    43261 aaatgaacgt ccacatcaac agacggtgat tatcacgggt cgaggctgtc atcgggatat
    43321 tcttgaactg gcggatacgg taagtgagct gcgccccatc agacatgcgt ttgatgccgg
    43381 tgttaaggcg caaataggga ttgattatta atctgtaaaa atgccggata tgacgtccgg
    43441 catactttga ttaaccgtta ttacgcccgc cagaacgacg actgccgctc acctgactgt
    43501 gacgtttcac cgcacgacga atctgattag ccttcatgcg acgacggtct ttttctacag
    43561 cgactttaga gctggtttct ggcggaagct ccaccagttc gcgcagatag ttagtctggg
    43621 cgaggtccag ctccgtccag ccaccgcgtg gcagaccttt tggcagtggg atatcaccgt
    43681 aacgaacacg gatcaggcgg ctcacctgca caccaaccgc ttcccacaga cgacgaactt
    43741 cacggttacg gccttcggtc agagtcacgt tgtaccactg gttgatccct tcgccgccgc
    43801 tgaacttgat ggttttaaaa gctgccggac catcttccag ttgcacgcca cgactcaaat
    43861 cacgcagttt cgcgtcgtca acctgaccaa atacacgcac ggcgtattca cgttcaactt
    43921 cacggcttgg atgcattaaa cggtttgcca gttcaccatc ggtggtgaac agcagcagac
    43981 cacaggtgtt aacgtccaga cgacctacgg caatccagcg tgcgccacgc agctttggca
    44041 gacggtcaaa caccgtcgga cgcccttccg gatcgttacg ggtacacaac tcaccttccg
    44101 gtttgtaata ggccagaacg cgacaaattt gttcagcgga ctcacgtacc gaaatcagat
    44161 gaccatcgat acgaattttc agcccagggg taacttcaac gcgatcgccg agtttagcaa
    44221 ttttgccatc aacactcaca cgaccggctt caataataga ttcgatttca cggcgggagc
    44281 cgtggccagc gcgggccagc actttctgta atttttcgct catagagctt ccttttggtg
    44341 tcgccttcac aggcgtcgaa caggagaatc aatggcgccg tatagcgcca ttttaaggcc
    44401 gcgtagtata cagagttacg cacttataag aaaggcttca catcacctac gccttcacgc
    44461 accacgaccg gcgtatcatc ggtaagatca ataaccgttg ttggtttctg cccgagatag
    44521 ccgccgtgaa taatcaaatc tacctgtttt tccagacggt ctttaatttc ttcgggatcg
    44581 gattcggtaa attcgctgcc tggcagcatt agcgaagtgg aaagcatcgg ttcgcccagt
    44641 gcttcaagca acgcctgcgc aatcgggtta gaaggcacac gcataccgat ggttttgcgt
    44701 ttctcctgca acagacggcg tggcacttcc ttcgtcccct tcaggatgaa ggtatagttg
    44761 cccggcgtgt tgtttttcat caaacgaaac gcaacgttat ccacgaatga ataggtcgag
    44821 agttcagaaa gatcgcgaca catcagggta aagttgtgac cgtccggcag ctggcgaata
    44881 cgacaaatac gctccatggc gtttttatct tcaattttac agccgagcgc atagccggaa
    44941 tcagttggat agacaatcac cccgccttta cgcacgatct ccaccgcctg gttgatcaga
    45001 cgttgctgcg ggttatcagg atgaatataa aaaaactggc tcataattcc ctctctgtgg
    45061 tgttctgcgg ctgttcccat agctgccaga cgccttcaac gcctgcgggc agccagagtt
    45121 tacgacccag ttcgatccac gggcatggct gatgaaaatc agatccttgt gatgcccata
    45181 aatgatgctg acgcgcaagt gtggccagct gggtgcgttc attgggcgat tgctggcact
    45241 gcgcgacttc catcgcgtcg ccgtggtgtt cggcaaaatg cgcaaccaat cttttcagcc
    45301 atttagcact aagattgtac cgcccaggat gagctagcac cgccttaccg ccagaatgat
    45361 gaatgacatc aatagcttgt tctattgtac accactgtgg cggaacgtat ccggttttcc
    45421 cgcgcgccag atacttttta aagacatctg ccattgaact ggctttgccg cactccacaa
    45481 ggaaacgcgc aaaatgaccg cgcgtcactg cgccgccctg cgccagtcgt tgtgcgcctt
    45541 ccagcgcccc aggtatttgc gctttttcaa gtctttcggc aatttgctgc gcccgctgat
    45601 tgcgccgttc tgtctgctgc gcgaggaact cacacattag cggatgagta atatcaatat
    45661 tcagcccgac aatatgaatt tcatgatttt cccagaccgt ggaaatttcc acgccgggaa
    45721 taagattcag cgccagtccc gaacgtgaaa tttcctctct tgctggcgcg attgcagctg
    45781 tggtgtcatg gtcggtgatc gccagggtgc cgacgcgcat ctcgactgca cggtgcacca
    45841 atgcttctgg cgtcaggcag ccatcggaag ctgtggtatg gctgtgcagg tcgtaaatca
    45901 ctgcataatt cgtgtcgctc aaggcgcact cccgttctgg ataatgtttt ttgcgccgac
    45961 atcataacgg gtctggcaaa tattctgaaa tgagctgttg acaattaatc atcgaaccag
    46021 ttaactagta cgcaagttca cgtaaaaagg gtatcgacaa tgaaagcaat tttcgtactg
    46081 aaaggttggt ggcgcacttc ctgaaacggg cagtgtattc accatgcgta aagcaatcag
    46141 atacccagcc cgcctaatga gcgggctttt ttttgaacaa aattagagaa taacaatgca
    46201 aacacaaaaa ccgactctcg aactgctaac ctgcgaaggc gcttatcgcg acaacccgac
    46261 tgcgcttttt caccagttgt gtgggaatcg tccggcaacg ctgctgctgg aatccgcaga
    46321 tatcgacagc aaagatgatt taaaaagcct gctgctgata gacagtgcgc tgcgcattac
    46381 agcattaggt gacaccgtca caattcaggc actttccggc aatggcgaag ccctgctggc
    46441 actactggat aacgccctgc ctgcgggtgt ggaaagtgaa caatcaccaa actgccgtgt
    46501 gctgcgcttc ccccctgtca gtccactgct ggatgaagac gcccgcttat gctccctttc
    46561 ggtttttgac gctttccgtt tattgcagaa tctgttgaat gtaccgaagg aagaacgaga
    46621 agcaatgttc ttcggcggcc tgttctctta tgaccttgtg gcgggatttg aagatttacc
    46681 gcaactgtca gcggaaaata actgccctga tttctgtttt tatctcgctg aaacgctgat
    46741 ggtgattgac catcagaaaa aaagcacccg tattcaggcc agcctgtttg ctccgaatga
    46801 agaagaaaaa caacgtctca ctgctcgcct gaacgaacta cgtcagcaac tgaccgaagc
    46861 cgcgccgccg ctgccagtgg tttccgtgcc gcatatgcgt tgtgaatgta atcagagcga
    46921 tgaagagttc ggtggcgtag tgcgtttgtt gcaaaaagcg attcgcgctg gagaaatttt
    46981 ccaggtggtg ccatctcgcc gtttctctct gccctgcccg tcaccgctgg cggcctatta
    47041 cgtgctgaaa aagagtaatc ccagcccgta catgtttttt atgcaggata atgatttcac
    47101 cctatttggc gcgtcgccgg aaagctcgct caagtatgat gccaccagcc gccagattga
    47161 gatctacccg attgccggaa cacgcccacg cggtcgtcgc gccgatggtt cactggacag
    47221 agatctcgac agccgtattg aactggaaat gcgtactgac cataaagagc tgtctgaaca
    47281 tctgatgctg gttgatctcg cccgtaatga tctggcacgc atttgcaccc ctggcagccg
    47341 ctacatcgcc gatctcacca aagttgaccg ttattcctat gtgatgcacc tcgtctctcg
    47401 cgtagtcggc gaactgcgtc acgatcttga cgccctgcac gcttatcgcg cctgtatgaa
    47461 tatggggacg ttaagcggtg cgccgaaagt acgcgctatg cagttaattg ccgaggcgga
    47521 aggtcgtcgc cgcggcagct acggcggcgc ggtaggttat ttcaccgcgc atggcgatct
    47581 cgacacctgc attgtgatcc gctcggcgct ggtggaaaac ggtatcgcca ccgtgcaagc
    47641 gggtgctggt gtagtccttg attctgttcc gcagtcggaa gccgacgaaa cccgtaacaa
    47701 agcccgcgct gtactgcgcg ctattgccac cgcgcatcat gcacaggaga ctttctgatg
    47761 gctgacattc tgctgctcga taatatcgac tcttttacgt acaacctggc agatcagttg
    47821 cgcagcaatg gtcataacgt ggtgatttac cgcaaccata ttccggcgca gaccttaatt
    47881 gaacgcctgg cgacgatgag caatccggta ctgatgcttt ctcctggccc cggtgtgccg
    47941 agcgaagccg gttgtatgcc ggaactcctc acccgcttgc gtggcaagct gcccattatt
    48001 ggcatttgcc tcggacatca ggcaattgtc gaagcttacg ggggctatgt cggtcaggcg
    48061 ggcgaaattc ttcacggtaa agcgtcgagc attgaacatg acggtcaggc gatgtttgcc
    48121 ggattaacaa acccgctgcc ggtggcgcgt tatcactcgc tggttggcag taacattccg
    48181 gccggtttaa ccatcaacgc ccattttaat ggcatggtga tggcagtacg tcacgatgcg
    48241 gatcgcgttt gtggattcca gttccatccg gaatccattc tcaccaccca gggcgctcgc
    48301 ctgctggaac aaacgctggc ctgggcgcag cagaaactag agccagccaa cacgctgcaa
    48361 ccgattctgg aaaaactgta tcaggcgcag acgcttagcc aacaagaaag ccaccagctg
    48421 ttttcagcgg tggtgcgtgg cgagctgaag ccggaacaac tggcggcggc gctggtgagc
    48481 atgaaaattc gcggtgagca cccgaacgag atcgccgggg cagcaaccgc gctactggaa
    48541 aacgcagcgc cgttcccgcg cccggattat ctgtttgctg atatcgtcgg tactggcggt
    48601 gacggcagca acagtatcaa tatttctacc gccagtgcgt ttgtcgccgc ggcctgtggg
    48661 ctgaaagtgg cgaaacacgg taaccgcagc gtctccagta aatccggctc gtcggatctg
    48721 ctggcggcat tcggtattaa tcttgatatg aacgccaata aatcgcgcca ggcgctggac
    48781 gagttaggcg tatgtttcct ctttgcgccg aaatatcaca ccggattccg ccacgcgatg
    48841 ccggttcgcc agcaactgaa aacccgcacc ctgttcaatg tgctggggcc attgattaac
    48901 ccggcgcatc cgccgctggc gttaattggt gtttatagtc cggaactggt gctgccgatt
    48961 gccgaaacct tgcgcgtgct ggggtatcaa cgcgcggcgg tggtgcacag cggcgggatg
    49021 gatgaagttt cattacacgc gccgacaatc gttgccgagc tgcatgacgg caaaattaag
    49081 agctatcaat tgaccgctga agattttggc ctgactccct accaccagga gcaactggca
    49141 ggcggaacac ctgaagaaaa ccgtgacatt ttaacacgct tgttacaagg taaaggcgac
    49201 gccgcccatg aagcagccgt cgctgcgaac gtcgccatgt taatgcgcct gcatggccat
    49261 gaagatctgc aagccaatgc gcaaaccgtt cttgaggtac tgcgcagtgg ttccgcttac
    49321 gacagagtta ccgcgctggc ggcacgaggg taaatgatgc aaaccgtttt agcgaaaatc
    49381 gtcgcagaca aggcgatttg ggtagaaacc cgcaaacagc agcaaccgct ggccagtttt
    49441 cagaatgagg ttcagccgag cacacgacat ttttatgatg cgctacaggg tgcacgcacg
    49501 gcgtttattc tggagtgtaa aaaagcgtcg ccgtcaaaag gcgtgatccg tgatgatttc
    49561 gatccggcac gcattgccgc catttataaa cattacgctt cggcaatttc ggtgctgact
    49621 gatgagaaat attttcaggg gagctttgat ttcctcctca tcgtcagcca aatcgccccg
    49681 cagccgattt tatgtaaaga cttcattatc gacccttacc agatctatct ggcgcgctat
    49741 taccaggccg atgcctgctt attaatgctt tcagtactgg atgacgacca atatcgccag
    49801 cttgcagccg tcgctcacag tctggagatg ggggtgctga ccgaagtcag taatgaagag
    49861 gaactggagc gcgccattgc attaggggca aaggtcgttg gcatcaacaa ccgcgatctg
    49921 cgtgatttgt cgattgatct caaccgtacc cgcgagcttg cgccgaaact ggggcacaac
    49981 gtaacggtaa tcagcgaatc cggcatcaat acttacgctc aggtgcgcga gttaagccac
    50041 ttcgctaacg gttttctgat tggttcggcg ttgatggccc atgacgattt gcacgccgcc
    50101 gtgcgccggg tgttgctggg tgagaataaa gtatgtggcc tgacgcgtgg gcaagatgct
    50161 aaagcagctt atgacgcggg cgcgatttac ggtgggttga tttttgttgc gacatcaccg
    50221 cgttgcgtca acgttgaaca ggcgcaggaa gtgatggctg cggcaccgtt gcagtatgtt
    50281 ggcgtgttcc gcaatcacga tattgccgat gtggtggaca aagctaaggt gttatcgctg
    50341 gcggcagtgc aactgcatgg caatgaagat cagctgtata tcgatacgct gcgtgaagct
    50401 ctgccagcac atgttgccat ctggaaagca ttaagcgtcg gtgaaaccct gcccgcccgc
    50461 gagtttcagc acgttgataa atatgtttta gacaacggcc agggtggaag cgggcaacgt
    50521 tttgactggt cactattaaa tggtcaatcg cttggcaacg ttctgctggc ggggggctta
    50581 ggcgcagata actgcgtgga agcggcacaa accggctgcg ccggacttga ttttaattct
    50641 gctgtagagt cgcaaccggg catcaaagac gcacgtcttt tggcctcggt tttccagacg
    50701 ctgcgcgcat attaaggaaa ggaacaatga caacattact taacccctat tttggtgagt
    50761 ttggcggcat gtacgtgcca caaatcctga tgcctgctct gcgccagctg gaagaagctt
    50821 ttgtcagcgc gcaaaaagat cctgaatttc aagcccagtt caacgacctg ctgaaaaact
    50881 atgccgggcg tccaaccgca ctgaccaaat gccagaacat tacagccggg acgaacacca
    50941 cgctgtatct caagcgtgaa gatttgctgc acggcggcgc acataaaacc aaccaggtgc
    51001 tggggcaggc gttgctggcg aagcggatgg gtaaaaccga aatcatcgcc gaaaccgggg
    51061 ccggtcagca tggcgtggcg tcggcccttg ccagcgccct gctcggcctg aaatgccgta
    51121 tttatatggg tgccaaagac gttgaacgcc agtcgcctaa cgtttttcgt atgcgcttaa
    51181 tgggtgcgga agtgatcccg gtacatagcg gttccgccac gctgaaagat gcctgtaatg
    51241 aggcgctacg cgactggtcc ggcagttatg aaaccgcgca ctatatgctg ggcaccgcgg
    51301 ctggcccgca tccttatccg accattgtcc gtgagtttca gcggatgatt ggcgaagaaa
    51361 ccaaagcgca gattctggaa agagaaggtc gcctgccgga tgccgttatc gcctgtgttg
    51421 gcggcggttc gaatgccatc ggcatgtttg ctgatttcat caatgaaacc aacgtcggcc
    51481 tgattggtgt ggagcctggt ggtcacggta tcgaaactgg cgagcacggc gcaccgctaa
    51541 aacatggtcg cgtgggcatt tatttcggta tgaaagcgcc gatgatgcaa accgaagacg
    51601 ggcagattga agaatcttac tccatctccg ccggactgga tttcccgtct gtcggtccac
    51661 aacacgcgta tcttaacagc actggacgcg ctgattacgt gtctattacc gatgatgaag
    51721 cccttgaagc cttcaaaacg ctgtgcctgc acgaagggat catcccggcg ctggaatcct
    51781 cccacgccct ggcccatgcg ctgaaaatga tgcgcgaaaa cccggataaa gagcagctac
    51841 tggtggttaa cctttccggt cgcggcgata aagacatctt caccgttcac gatattttga
    51901 aagcacgagg ggaaatctga tggaacgcta cgaatctctg tttgcccagt tgaaggagcg
    51961 caaagaaggc gcattcgttc ctttcgtcac gctcggtgat ccgggcattg agcagtcatt
    52021 gaaaattatc gatacgctaa ttgaagccgg tgctgacgcg ctggagttag gtatcccctt
    52081 ctccgaccca ctggcggatg gcccgaccat tcaaaacgcc actctgcgcg cctttgcggc
    52141 aggtgtgact ccggcacaat gttttgaaat gctggcactg attcgccaga aacacccgac
    52201 cattcccatt ggcctgttga tgtatgccaa tctggtgttt aacaaaggca ttgatgagtt
    52261 ttatgcccag tgcgagaaag tcggcgtcga ttcggtgctg gttgccgatg tgccagttga
    52321 agagtccgcg cccttccgcc aggccgcgtt gcgccataat gtcgcaccta tcttcatctg
    52381 cccgccaaat gccgatgacg acctgctgcg ccagatagcc tcttacggtc gtggttacac
    52441 atatttgctg tcacgagcgg gcgtgaccgg cgcagaaaac cgcgccgcgt tacccctcaa
    52501 tcatctggtt gcgaagctga aagagtacaa cgctgcacct ccattgcagg ggtttggtat
    52561 ttccgccccg gatcaggtaa aagcagcgat tgatgcagga gctgcgggcg cgatttctgg
    52621 ttcggccatt gttaaaatca tcgagcaaca tattaatgag ccagagaaaa tgctggcggc
    52681 actgaaagtt tttgtacaac cgatgaaagc ggcgacgcgc agttaatccc acagccgcca
    52741 gttccgctgg cggcatttta actttcttta atgaagccgg aaaaatccta aattcattta
    52801 atatttatct ttttaccgtt tcgcttaccc cggtcgaacg tcaacttacg tcatttttcc
    52861 gcccaacagt aatataatca aacaaattaa tcccgcaaca taacaccagt aaaatcaata
    52921 attttctcta agtcacatat tcctcaggta attgttaata tatccagaat gttcctcaaa
    52981 atatatttcc ctctatcttc tcgttgcgct taatttgact aattctcatt agtgactaat
    53041 tttaatgagt gtcgacacac aacactcata ttaatgaaac aatgcaacgc aacgggagaa
    53101 ataacatggc cgaacatcgt ggtagttcag gaaatttcgc cgaagaccgt gagaaggcat
    53161 ccgacgcagg ccgtaaaggc ggtcagcata gcggcggtaa ttttaaaaat gatccgcaac
    53221 gcgcatctga agcgggtaaa aaaggcggtc aacaaagcgg tggtaataaa tcaggcaaat
    53281 cctgattcac cgttaattat taatatcagc tgataaagat aattgcatct gcgggccgta
    53341 tggctcgcag tgatttcact ggagaaaaat atgaatatga agaccattga agatgtattt
    53401 attcaactgc tttcagatac ctacagcgca gaaaaacaat taacccgggc actggcaaaa
    53461 ctcgcaagag caacatcaaa tgaaaaatta agtcaggctt ttcatgcgca cctcgaggaa
    53521 actcatggac agattgaacg aattgatcaa gttgtggaat cagaatcaaa tctgaaaatt
    53581 aagcgcatga aatgtgtggc aatggaaggt cttattgaag aagctaatga ggtcatcgaa
    53641 agtaccgaga aaaacgaagt gcgtgatgcc gcactgattg ccgcagcaca gaaagtcgag
    53701 cattatgaga ttgccagtta cgggacatta gcgacgctgg ctgaacaatt aggttaccgt
    53761 aaagcagcga agcttctgaa agaaaccctg gaagaagaaa aggccaccga catcaaactg
    53821 actgatctgg cccttaataa cgtaaataag aaagccgaaa ataaagcctg aaatatgaat
    53881 tttaactttt agtcatttta taaagaggac attttcatga atcgtattga acattatcat
    53941 gactggttac gtgacgccca cgcaatggaa aagcaagccg aatctatgct tgaatccatg
    54001 gccagccgta tagataatta tcctgaacta cgcgctcgta ttgaacaaca tcttagtgaa
    54061 accaaaaacc agattgttca actggaaact attcttgatc gtaatgacat ttcacgttca
    54121 gtcattaaag attccatgag taaaatggct gcgcttgggc agtcaatcgg tggtatattc
    54181 ccttctgatg aaatagttaa aggctctatt ggcggatatg tcttcgagca atttgaaatc
    54241 acctgttaca cctcactatt agcagcagca aaaaatgccg gtgatacagt ctcaattccg
    54301 atcatcgaag cgattttaaa tgaggaaaag caaatggccg actggctgat tcagcatatt
    54361 ccgcaaacaa ctgagaaatt tttaattcgt tctgaaactg atggcgtaga agcgaagaaa
    54421 taataccctt tataccatgt ccttattgac cccgtatatt acggggtcgt ttttgtgcgg
    54481 aattaaaaac gatatcctgc tgagaacata aacacccacg gatcgaggcg tacgctatcg
    54541 tgttgctgtg caccgcctag cttataattg gcggtggtat cgatatccat gtaccacact
    54601 gacatgttaa ccaaccagtc acggttaatc agataatcaa cccccacctg cccggcagct
    54661 ccccaggaat ctttcagact gagatcggaa agccccgcct ctttgccatg atcgttaaat
    54721 ccattatcaa agaaggtggt gtagttaata ccggccccaa cgtaaggacg gaatttgctg
    54781 ctggcatcac caaaatacca ctgcgccatc agtgttggcg gcagatggtg aacggttgca
    54841 atatcgccgg tcgcccgggt gccgatttta tggcggaacg gcgtcgctgc cagtaattcc
    54901 acaccaatgt tgtcggtcgc catataagta aacgtcaagc ccagttgagt gttattggtc
    54961 acgctgaatc cacccagact tcctaacgta ccaccagcac cttctgttgg acgtacggtt
    55021 gcagaacctg cacgcataaa aaattcgcct gcttcatgcg caaaggcact gccagaaaga
    55081 agagttgtta ctgccaaagc cgccactgtt aactttttca tatccgctcc gtcgttatgg
    55141 ttataaaaac gtgacgaata tacctacatt ggagtaacaa atgatcctac atagatcaca
    55201 ttacatatga taattcacca tttattgatc tggattaatt tagaagttgc attgagaatc
    55261 ctggttaatg tgatttaaat caatttttaa atattttctc tacgcaaata ttaactcgat
    55321 cgccacttgc cgcgctttgc ccacgcctga aggccagcaa ttacctccgc tttacaaaga
    55381 tatgcttttc catacaagac ctgatgctgc cacccgaggc ttaaccaggg tacaattgcc
    55441 cgctaattaa cacctgcata aaactcaagg agagtgcatg tctatcacgg cgcagtccgt
    55501 ataccgtgac accggaaatt tcttccgtaa tcaatttatg accattctgt tggtatcgtt
    55561 gctatgtgcg tttatcacag tggtgttagg gcatgttttc tcacccagtg atgcacagct
    55621 tgcgcagctc aatgacggcg tgcccgttag cggcagtagt gggttgttcg acctggttca
    55681 gaatatgtca ccggaacagc aacaaatttt gctgcaggct tcagcggcgt ccactttttc
    55741 agaattaatc ggtaacgcca ttctcgccgg aggcgtaata ttaattatcc agctggtgtc
    55801 tgcgggtcag agagtcagtg cgctgcgagc tattggtgcc agtgcgccga tattgcccaa
    55861 gttatttatt ctgatttttc tgactaccct tttagtacag attggcatca tgctggtggt
    55921 cgttccggga attatcatgg ccattttact ggccctggca ccggtgatgc tggtacagga
    55981 caaaatgggc atttttgcct cgatgcgtag cagtatgcgg ctgacttggg cgaatatgcg
    56041 tctggtggca cccgcagtac tgagctggtt gctggcaaaa acactgttgc tgctttttgc
    56101 ctcttctttt gccgcattaa ccccggaaat tggtgccgta ctggcgaaca ccttgagcaa
    56161 cctgatttct gccatattgc tcatctatct gttccgcctg tatatgttga ttcgccaata
    56221 accttaagca tttgattacg gaatcgtaaa atgaagcagt ttcttgattt tttaccgctg
    56281 gttgtctttt tcgcgtttta caagatttat gacatctatg cggctactgc agcgctgatc
    56341 gtcgccacgg cgattgtgct tatctatagc tgggttcgct ttcgtaaggt tgagaagatg
    56401 gccctgatca cttttgttct ggtggtcgtc ttcggtggct tgacgctgtt cttccacaat
    56461 gatgagttta ttaaatggaa ggttacagtc atttatgctc tgtttgcggg agccctgtta
    56521 gtcagccaat gggtgatgaa aaagccgcta attcagcgga tgctgagtaa agaactcacg
    56581 ctgccgcaac cggtatggtc gaagctaaat ctggcctggg ctgttttctt tatcctttgc
    56641 ggtctggcaa acatctacat cgcattctgg ctgccgcaaa atatttgggt caactttaaa
    56701 gtctttggcc tgaccgccct taccttaatc ttcacattgt taagcggtat ctatatctac
    56761 cgccacatgc cgcaggaaga taaatcctaa ccagactgcc agtccttcgg ggctggcgct
    56821 gtatctcctc tgttctcctc aggaaaatca tagtagcatc gcgcctgtga ttttcctttt
    56881 aagtcggttt taccatgtct acaacacata acgtccctca gggcgatctt gttttacgta
    56941 ctttagccat gcccgccgat accaatgcca atggtgacat ctttggtggt tggttaatgt
    57001 cacaaatgga tattggcggc gctattctgg caaaagaaat tgcccacggt cgcgtagtga
    57061 ctgtgcgggt tgaaggaatg actttcttac ggccggttgc ggtcggcgat gtggtgtgct
    57121 gctatgcacg ctgtgtccag aaagggacga catcggtcag cattaatatt gaagtgtggg
    57181 tgaaaaaagt agcgtctgaa ccaatcgggc aacgctataa agcgacagaa gcattattta
    57241 agtatgtagc ggttgatcct gaaggaaaac ctcgcgcctt acctgttgag taatagtcaa
    57301 aagcctccgg tcggaggctt ttgactttct gcttactgaa tttcggtggt gccgttaatt
    57361 ttaaacagga tattcaccac aatcccactg cctggcttac ccggctcata acgccatctg
    57421 cgcatcgcat ttttcacctc acgctcaaac atgttcgcag gcttggctga gaggatttgt
    57481 acgttatcca cgcgaccatc cggcgtgacg tcaaatttaa ctttaacctg cccttcaatg
    57541 cgcaatgcct gtgctcgtgc cggatactgc ggctgattac ggcttaatgc gcgtggtcct
    57601 gaagccacac tggtaaccgg cttgctggtt gcagccgttg ccgtacttga tgtggggcgc
    57661 gcgggtgccg tattttcaaa cggtgatgcc ggacgcgact ctacgggttt gacatcgcgt
    57721 tttggctgct cctgtacctt tttcaccggc ttcggttttg gcttaggttt cggcttcggc
    57781 ttttcaatga ccaccggtgc ttcttttggc ggttcgggga tcggctcagg ttccggttct
    57841 ggctctacca ccggctccgg tggcggctga acggcttgtg gcggttcgag atcagcaggc
    57901 gcaaccatcg tgacagaaat tggttgcgca ggcgcaggta gttcaataac ctgatgtacc
    57961 gaggtataga gcagccccgc cacaacagca ccatgaatgc agaccgaaag taacgtcggc
    58021 caggggaagc ggcgaggtaa atcaagggtt attgaagtca taatcatttc agtagaaaaa
    58081 ctaggcctcg attttaaatg caaatagcaa tcatattcaa taaggcaaga cgatcaggat
    58141 cagtgaaaat gtcatttttg gcgtaattac ccttacttta tgcatgacct taacaaagac
    58201 atcatctttc cgttgcagat cttcgccctt tcaaataacg tactttacaa ctttcccgaa
    58261 caaggagttg tgcccgtgtt gtatgttatt tacgctcaag acaaagctga tagcctcgaa
    58321 aaacgccttt ccgttcgtcc ggcacattta gcacgtttac agttactgca tgatgaaggt
    58381 cgtttgttga cagctggtcc aatgccagcg gtagacagta acgatccagg ggctgcggga
    58441 tttactggct caaccgttat tgctgaattt gaatcgctgg aagcagctca ggcctgggcc
    58501 gatgctgacc cgtacgtagc ggcaggagtc tatgaacacg tttcagtgaa accatttaag
    58561 aaagtgttct gag
//
LOCUS       DAEBXN010000011        58032 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.011, whole genome shotgun sequence.
ACCESSION   DAEBXN010000011 DAEBXN010000000
VERSION     DAEBXN010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58032
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.011"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(24..278)
                     /locus_tag="KJL99_000663"
     CDS             complement(24..278)
                     /locus_tag="KJL99_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HBD4435518.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            433..1005
                     /gene="yeiP"
                     /locus_tag="KJL99_000664"
     CDS             433..1005
                     /gene="yeiP"
                     /locus_tag="KJL99_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HBD4435519.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLAKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            1228..2694
                     /locus_tag="KJL99_000665"
     CDS             1228..2694
                     /locus_tag="KJL99_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBD4435520.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERSLTPFTVLSCDNIPDNGHVVKNAVLRMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAYISDCMQDRVFRYAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            2812..3798
                     /gene="yeiR"
                     /locus_tag="KJL99_000666"
     CDS             2812..3798
                     /gene="yeiR"
                     /locus_tag="KJL99_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HBD4435521.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            3837..4550
                     /gene="lpxT"
                     /locus_tag="KJL99_000667"
     CDS             3837..4550
                     /gene="lpxT"
                     /locus_tag="KJL99_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HBD4435522.1"
                     /translation="MIKNLPQIVLLNIVGPALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFMVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            4962..5528
                     /gene="mepS"
                     /locus_tag="KJL99_000668"
     CDS             4962..5528
                     /gene="mepS"
                     /locus_tag="KJL99_000668"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HBD4435523.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            5709..7265
                     /locus_tag="KJL99_000669"
     CDS             5709..7265
                     /locus_tag="KJL99_000669"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HBD4435524.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPDREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVTEGVETPEQARWLSERGVNFMQGYWISHPLPLDDFVR
                     WLKKPYTPQW"
     gene            7347..9161
                     /locus_tag="KJL99_000670"
     CDS             7347..9161
                     /locus_tag="KJL99_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBD4435525.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYTFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFLTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYHVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRAVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRNRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            9162..10256
                     /locus_tag="KJL99_000671"
     CDS             9162..10256
                     /locus_tag="KJL99_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HBD4435526.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HMFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            10256..11281
                     /locus_tag="KJL99_000672"
     CDS             10256..11281
                     /locus_tag="KJL99_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBD4435527.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            11283..12872
                     /gene="yejF"
                     /locus_tag="KJL99_000673"
     CDS             11283..12872
                     /gene="yejF"
                     /locus_tag="KJL99_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HBD4435528.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(12876..13220)
                     /locus_tag="KJL99_000674"
     CDS             complement(12876..13220)
                     /locus_tag="KJL99_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4435529.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(13553..14743)
                     /gene="bcr"
                     /locus_tag="KJL99_000675"
     CDS             complement(13553..14743)
                     /gene="bcr"
                     /locus_tag="KJL99_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HBD4435530.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVSA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIALLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISAPLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(14771..15466)
                     /gene="rsuA"
                     /locus_tag="KJL99_000676"
     CDS             complement(14771..15466)
                     /gene="rsuA"
                     /locus_tag="KJL99_000676"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HBD4435531.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            15616..17376
                     /locus_tag="KJL99_000677"
     CDS             15616..17376
                     /locus_tag="KJL99_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBD4435532.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDNPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            17501..17785
                     /gene="rplY"
                     /locus_tag="KJL99_000678"
     CDS             17501..17785
                     /gene="rplY"
                     /locus_tag="KJL99_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HBD4435533.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(17924..18931)
                     /gene="yejK"
                     /locus_tag="KJL99_000679"
     CDS             complement(17924..18931)
                     /gene="yejK"
                     /locus_tag="KJL99_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HBD4435534.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            19113..19340
                     /locus_tag="KJL99_000680"
     CDS             19113..19340
                     /locus_tag="KJL99_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HBD4435535.1"
                     /translation="MPQISRYSNEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFASALQSSINEDKAH"
     gene            19360..21120
                     /gene="pbgA"
                     /locus_tag="KJL99_000681"
     CDS             19360..21120
                     /gene="pbgA"
                     /locus_tag="KJL99_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HBD4435536.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDDQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQIKRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            21195..21271
                     /locus_tag="KJL99_000682"
     tRNA            21195..21271
                     /locus_tag="KJL99_000682"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21229..21231,aa:Pro,seq:ggg)
     gene            complement(21374..23965)
                     /gene="yejO"
                     /locus_tag="KJL99_000683"
     CDS             complement(21374..23965)
                     /gene="yejO"
                     /locus_tag="KJL99_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HBD4435537.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVILE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGPANDTTIKGGRLIVEKDGGAVFAAIEKGGLLEVKEGG
                     FALAVDQKTGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            24285..24932
                     /gene="narP"
                     /locus_tag="KJL99_000684"
     CDS             24285..24932
                     /gene="narP"
                     /locus_tag="KJL99_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HBD4435538.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGEGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRTGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(25143..26195)
                     /gene="ccmH"
                     /locus_tag="KJL99_000685"
     CDS             complement(25143..26195)
                     /gene="ccmH"
                     /locus_tag="KJL99_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HBD4435539.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(26192..26749)
                     /gene="dsbE"
                     /locus_tag="KJL99_000686"
     CDS             complement(26192..26749)
                     /gene="dsbE"
                     /locus_tag="KJL99_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HBD4435540.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(26746..28689)
                     /gene="ccmF"
                     /locus_tag="KJL99_000687"
     CDS             complement(26746..28689)
                     /gene="ccmF"
                     /locus_tag="KJL99_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HBD4435541.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLATAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(28686..29165)
                     /gene="ccmE"
                     /locus_tag="KJL99_000688"
     CDS             complement(28686..29165)
                     /gene="ccmE"
                     /locus_tag="KJL99_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HBD4435542.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(29162..29371)
                     /gene="ccmD"
                     /locus_tag="KJL99_000689"
     CDS             complement(29162..29371)
                     /gene="ccmD"
                     /locus_tag="KJL99_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HBD4435543.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(29368..30105)
                     /gene="ccmC"
                     /locus_tag="KJL99_000690"
     CDS             complement(29368..30105)
                     /gene="ccmC"
                     /locus_tag="KJL99_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HBD4435544.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(30147..30809)
                     /gene="ccmB"
                     /locus_tag="KJL99_000691"
     CDS             complement(30147..30809)
                     /gene="ccmB"
                     /locus_tag="KJL99_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HBD4435545.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(30806..31423)
                     /gene="ccmA"
                     /locus_tag="KJL99_000692"
     CDS             complement(30806..31423)
                     /gene="ccmA"
                     /locus_tag="KJL99_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HBD4435546.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQTLLWIGHQPGIKTRLTALENLHFYHRDG
                     DAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(31442..32044)
                     /gene="napC"
                     /locus_tag="KJL99_000693"
     CDS             complement(31442..32044)
                     /gene="napC"
                     /locus_tag="KJL99_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HBD4435547.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(32054..32503)
                     /gene="napB"
                     /locus_tag="KJL99_000694"
     CDS             complement(32054..32503)
                     /gene="napB"
                     /locus_tag="KJL99_000694"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HBD4435548.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(32500..33363)
                     /gene="napH"
                     /locus_tag="KJL99_000695"
     CDS             complement(32500..33363)
                     /gene="napH"
                     /locus_tag="KJL99_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HBD4435549.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(33350..34045)
                     /gene="napG"
                     /locus_tag="KJL99_000696"
     CDS             complement(33350..34045)
                     /gene="napG"
                     /locus_tag="KJL99_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HBD4435550.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(34052..36538)
                     /gene="napA"
                     /locus_tag="KJL99_000697"
     CDS             complement(34052..36538)
                     /gene="napA"
                     /locus_tag="KJL99_000697"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HBD4435551.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNSRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(36535..36798)
                     /gene="napD"
                     /locus_tag="KJL99_000698"
     CDS             complement(36535..36798)
                     /gene="napD"
                     /locus_tag="KJL99_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HBD4435552.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(36788..37282)
                     /gene="napF"
                     /locus_tag="KJL99_000699"
     CDS             complement(36788..37282)
                     /gene="napF"
                     /locus_tag="KJL99_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HBD4435553.1"
                     /translation="MKIDASRRGILTGCWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGTGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQAQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            37391..37555
                     /locus_tag="KJL99_000700"
     CDS             37391..37555
                     /locus_tag="KJL99_000700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4435554.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            37690..38178
                     /gene="eco"
                     /locus_tag="KJL99_000701"
     CDS             37690..38178
                     /gene="eco"
                     /locus_tag="KJL99_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708105.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HBD4435555.1"
                     /translation="MKTILPEVLFAAFATTSAWAAESVQPLEKIAPYPQAETGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAE
                     VR"
     gene            complement(38300..39331)
                     /locus_tag="KJL99_000702"
     CDS             complement(38300..39331)
                     /locus_tag="KJL99_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HBD4435556.1"
                     /translation="MNYGEITRQLIASLQKKSNFSLQLSSEVRALKRNDDNTWTVTVA
                     DLKNGTAQNIRAKFVFIGAGGAALKLLQESGIPEAKDYAGFPVGGQFLVSENPDVVNR
                     HLAKVYGKASVGAPPMSVPHIDTRVLDGKRVVLFGPFATFSTKFLKNGSLWDLMSSTT
                     TSNVMPMMHVGLDNFDLVKYLVSQVMLSEEDRFEALKEYYPQAKKEDWRLWQAGQRVQ
                     IIKRDAEKGGVLRLGTEVVSDQQGTIAALLGASPGASTAAPIMLNLLEKVFGDRVSSP
                     QWQATLKAIVPSYGRKLNGDVAATERELQYTSEVLGLKYDKPQAADSTPKPQLKPQPV
                     QKEVADIAL"
     gene            complement(39565..39924)
                     /locus_tag="KJL99_000703"
                     /pseudo
     CDS             complement(39565..39924)
                     /locus_tag="KJL99_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012906537.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
     gene            complement(40142..41785)
                     /gene="yojI"
                     /locus_tag="KJL99_000704"
     CDS             complement(40142..41785)
                     /gene="yojI"
                     /locus_tag="KJL99_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HBD4435557.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELGQGIILTIGSSAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAVDQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            complement(41861..42511)
                     /gene="alkB"
                     /locus_tag="KJL99_000705"
     CDS             complement(41861..42511)
                     /gene="alkB"
                     /locus_tag="KJL99_000705"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HBD4435558.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVAPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            complement(42511..43575)
                     /gene="ada"
                     /locus_tag="KJL99_000706"
     CDS             complement(42511..43575)
                     /gene="ada"
                     /locus_tag="KJL99_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HBD4435559.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            complement(43649..44704)
                     /gene="apbE"
                     /locus_tag="KJL99_000707"
     CDS             complement(43649..44704)
                     /gene="apbE"
                     /locus_tag="KJL99_000707"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HBD4435560.1"
                     /translation="MEISFTRVALLVAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            complement(44816..45937)
                     /gene="ompC"
                     /locus_tag="KJL99_000708"
     CDS             complement(44816..45937)
                     /gene="ompC"
                     /locus_tag="KJL99_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HBD4435561.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRSTDFFGL
                     VDGLNFAVQYQGKNGSPEGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNRYDERYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLGWA
                     NKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            46675..49347
                     /gene="rcsD"
                     /locus_tag="KJL99_000709"
     CDS             46675..49347
                     /gene="rcsD"
                     /locus_tag="KJL99_000709"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HBD4435562.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTRQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVEE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRLLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            49364..50014
                     /gene="rcsB"
                     /locus_tag="KJL99_000710"
     CDS             49364..50014
                     /gene="rcsB"
                     /locus_tag="KJL99_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HBD4435563.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(50214..53063)
                     /gene="rcsC"
                     /locus_tag="KJL99_000711"
     CDS             complement(50214..53063)
                     /gene="rcsC"
                     /locus_tag="KJL99_000711"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HBD4435564.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPCVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRDS"
     gene            complement(53338..54114)
                     /locus_tag="KJL99_000712"
     CDS             complement(53338..54114)
                     /locus_tag="KJL99_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HBD4435565.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(54119..55768)
                     /locus_tag="KJL99_000713"
     CDS             complement(54119..55768)
                     /locus_tag="KJL99_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HBD4435566.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPSVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QELIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            complement(55769..56328)
                     /locus_tag="KJL99_000714"
                     /pseudo
     CDS             complement(55769..56328)
                     /locus_tag="KJL99_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112876.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
     gene            complement(56332..56490)
                     /locus_tag="KJL99_000715"
     CDS             complement(56332..56490)
                     /locus_tag="KJL99_000715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4435567.1"
                     /translation="MTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGG
                     GEDWRWVG"
     gene            complement(56487..>58032)
                     /locus_tag="KJL99_000716"
     CDS             complement(56487..>58032)
                     /locus_tag="KJL99_000716"
                     /inference="COORDINATES: protein motif:HMM:NF012433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HBD4435568.1"
                     /translation="LTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRN
                     NVRTSSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDK
                     QGKAYFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDK
                     PAAGLFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQ
                     NGQVQDSISTKLSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFR
                     NNLDALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAG
                     PGARFTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPL
                     LHRALVLAWAQEMNLPCKTLLKGGDEAIARRGTKDEDFSEEDIRDINDSLILDTPESP
                     LADAVANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQT
                     AAILMV"
BASE COUNT        14197 a        15654 c        14259 g        13922 t
ORIGIN      
        1 tgcttactgc ggattaaaga tgtttaaggc gcggtcagca tttccagatc gataagccag
       61 gtcatggcct gctgacgcga gttaccgcac atttcagcgc tggcttgtaa gcctgcacag
      121 actttcggtc gcagtggcga ggtgaaaatt ttacagcgtt gctgttcatc aagctgaatg
      181 cagggcgtat tggcgggctt gccatctggc ataccgggaa tagggctgga aattgaaggg
      241 gcagtgcaac acgccccgca acccggacgg cattccatac gttgttctct taaggttaac
      301 caccggattg atgagcgcgc acagtaccac cttttttgca ccagcaaaag tgcgaatacc
      361 acttgcccga aaggcccgtc gcgagtactt tgtcgcgata tttttgacat tttcgactac
      421 aggaattttt cgatgccaag agcgaacgaa attaaaaaag gtatggtact gaattacaac
      481 ggcaaactgc tgctggctaa ggatattgat attcagtcgc ccactgcccg cggtgccgct
      541 acgctgtaca aaatgcgttt ttctgatgtc cgtaccgggc tgaaagtaga agagcgtttc
      601 aaaggtgatg atatcgttga caccgtgacg ctgacccgcc gttacgttga cttctcctat
      661 gtcgatggca acgaatatgt ctttatggat aaagaagact ataccccgta taccttcacc
      721 aaagatcaga ttgaagaaga gttgctgttt atgccagagg gcggcatgcc ggacatgcag
      781 gtgctgacct gggacggtca actgctggcg cttgagctgc cgcagaccgt tgatctggaa
      841 atcgttgaaa ccgcaccagg catcaaaggg gcatctgcca gcgcccgtaa caaaccggcg
      901 acattgagca ctggtctggt gattcaggta ccagaatact taagcccggg cgaaaaaatt
      961 cgtatccata tcgaagaacg ccgttatatg gggcgtgctg actaacttca gccgcatgca
     1021 gaaaagggat agctcaggct gtcccttttt taatttatta taccaatctt ctattttgcg
     1081 cttcctgaac accttacgcc ctcaattttc actcgttgat tgatcgccct cacacttcat
     1141 cgcattaaca atccagacca atttcaattg ctgtcatata actttacact gtcattgtta
     1201 atttatcgtt actaagacgt gactcctatg aagacaattg cctccgttac gctcccgcat
     1261 catgtacacg ctccacgcta cgatcgccag cagttgcaat cacgtatcgt tcattttggc
     1321 tttggcgcct ttcaccgcgc tcatcaggcg ttactgaccg atcgtgtgct gaatgcccag
     1381 ggcggcgact gggggatctg tgaaatcagc ttgttcagcg gtgatcaact gatgagccag
     1441 ctccgcgcac agaaccattt atataccgtg ctggagaaag gtgcggacgg caatcaggtg
     1501 ataattgtcg gtgccgttca cgaatgcctt aatgcaaaac tggattcctt agcggcaatt
     1561 attgagaaat tttgcgagcc acaggtggca attgtttccc tgacgattac cgaaaaaggc
     1621 tattgtattg acccggccac cggtgcactc gacaccagta atccgcggat tattcacgat
     1681 ctacaaaccc ctgaagaacc tcactccgca ccgggtattc tcgtcgaagc actgaaacgc
     1741 cgccgtgagc gcagccttac accgtttacc gtgctctcct gcgacaatat tcccgacaat
     1801 ggccatgtgg tgaaaaacgc ggtgctgaga atggcagaaa aacgttcgcc agaactcgcc
     1861 gggtggataa aagaacacgt cagttttccg ggaaccatgg tcgaccgcat tgttccggct
     1921 gcaaccgacg aatcactggc agaaatcagc cagcatctgg gggtaaatga tccctgcgcg
     1981 attagctgcg aaccgtttat ccagtgggtg gttgaagata acttcatcgc tgggcgtcct
     2041 gcctgggaag tcgcaggtgt acaaatggtg aatgatgtcc tgccatggga agagatgaaa
     2101 ctgcgtatgc ttaatggcag ccactctttt ctcgcttatc tgggttacct ctcaggattc
     2161 gcctatatca gtgattgcat gcaggatcgc gtatttcgct atgctgccag aacattaatg
     2221 ctggatgagc aagcgccgac actgcaaatt aaagatgtcg atttaacaca atatgcggat
     2281 aagttaattg cacgttttgc taatccgtcg ctgaaacata agacctggca aatcgcgatg
     2341 gatggcagcc agaaattacc gcaacgtatg ctggcaggta ttcgcataca tctggggcgt
     2401 gaaacggact ggtcgttgct ggcattaggc gttgctggct ggatgcgtta cgtcagcggc
     2461 gttgatgatg ccggaaatgc cattgatgtt cgcgatccgc ttagcgataa aattcgcgaa
     2521 cttgttgcgg gcagcagcag tgaacaacgc gtaaccgccc tgctttccct gcgtgaagtt
     2581 ttcggtgatg atctgccaga taacccgcat tttgtgcagg ccatcgaaca agcctggcaa
     2641 caaatcgtac aattcggcgc acatcaggcg ctattaaaca ccctcaaaat ttaacgattt
     2701 ctgcggttaa agcggatgaa gctcaccttc gtccgctctc cccttctctt ttctgccttt
     2761 tttagccagg attaacgctc agttaactta ccagagtatt gcggagccgt tgtgaccagg
     2821 accaacctca tcaccggttt tctcggcagc gggaaaacca cgtcgattct tcatctgtta
     2881 gcccataaag atcccaacga aaaatgggcg gtactggtta atgaatttgg ggaagtcgga
     2941 attgatggtg ctttgctcgc cgatagcggc gcattgctga aagagatccc cggcggctgc
     3001 atgtgctgcg ttaatggttt acccatgcag gtagggttga ataccttact gcgtcaggga
     3061 aaaccagacc gcttgttgat agagccgacc gggctgggcc atccgaaaca gatcctcgat
     3121 ctgttaaccg caccagtcta tgaaccgtgg atagatctgc gcgccacctt gtgcattctc
     3181 gatccacgcc tgctgctgga cgaaaaaagc gccagcaatg aaaacttccg tgaccagctg
     3241 gctgccgcag acatcattgt cgccaataaa tccgaccgtg cgacgcccga aagtgagcaa
     3301 gcgctacagc gttggtggca gcaaaatggt ggcgatcgac aattaattca cagtgagcat
     3361 gggaaagttg acggtcatct tctggatttg ccgcgtcgca atttagccga gttgcccgcc
     3421 agcgccgcgc attctcatca gcatgtcgtg aaaaaagggt tagcagcgtt aagcctgcca
     3481 gagcatcaac gctggcgtcg cagtctgaac agcgggcaag gatatcaggc ctgcggctgg
     3541 atattcgacg ctgatacggt attcgacacc attggcattc tggaatgggc gcgacttgca
     3601 ccggtggaac gcgtcaaagg cgtgctgcgt attcccgaag ggctggtacg aatcaaccgt
     3661 cagggcgatg acctgcacat tgaaacgcaa aacgttgcgc caccggacag ccgtattgag
     3721 ctgatttcca gcagcgaagc tgactggaat gccttgcaga gcgcgctgtt gaagcttcgt
     3781 ttagcgacta ccgcgtaagg ttgcctgcgt ttttcagtaa gataattaga gaaaatatga
     3841 ttaaaaattt gccgcaaata gtgttgttga atattgtcgg ccccgcgctg tttctttcct
     3901 ggtatattcc cgttaatcat ggattctggt tgccgattga tgcggatatt ttttatttct
     3961 ttaatcagaa actggtcgaa agtaaggcct ttttatggct ggttgcattg actaacaatc
     4021 gcgccttcga cggttgttca ctgctggcga tgggtatgtt gatgctgagt ttctggctga
     4081 aagaaaacgc ccctggcaga cgacgtatcg tgattatggg tctggtcatg ctattaactg
     4141 cagtggtatt aaaccagctg ggtcaggcat taattcctgt aaaacgggcc agcccaacat
     4201 tgacttttac cgatattaac cgcgtcagcg aactgctctc tgttcccacg aaagatgcct
     4261 cacgagatag ctttcccggc gatcacggca tgatgctgct tattttttcg gcattcatgt
     4321 ggcgttattt cggcaaagtt gcaggcctta tcgcccttat tatttttatg gtttttgcat
     4381 ttcccagagt aatgattggc gcacactggt ttactgacat cattgtcggt tcgatgaccg
     4441 tgatattgat cggtttgccc tgggtgttgc tgacgccatt aagtgatcga ttaatcacct
     4501 tttttgacaa atcactacca ggaaaaaaca aacatttcca aaacaaataa ctcacagtaa
     4561 ttaacatcat cagggttatt tttatagtga ggataatcct gatgatgcgc accgtgcttt
     4621 catctatcga acgcaaaaat cattctctaa gtaaatgaat ggattgcatg cgtttcactc
     4681 aattgtactt taattgacca accccgctta ttaactttct gtatcacttt ttcttataaa
     4741 aaatcatgta aaaccgctcg ccaagaccgc accaatcggg taatctcgaa ctcgttttgc
     4801 ctcggcggta gattatcctc acagcatata attttgtgcg ttagtccaca gatttggcct
     4861 taaggaattg tttcaacatg cccaggtaat tagtctcgtg tcgcttggca tttttttata
     4921 acgatatttg tcgttaagga cttcaaggga aaacaaacaa catggtcaaa tctcaaccga
     4981 ttttgagata tatcttgcgc gggattcccg cgattgcagt agcggttctg ctttctgcat
     5041 gtagtgcaaa taacaccgca aagaatatgc atcctgagac acgtgcagtg ggtagtgaaa
     5101 catcatcact gcaagcttct caggatgaat ttgaaaacct ggttcgtaat gtcgacgtaa
     5161 aatcgcgaat tatggatcag tatgctgact ggaaaggcgt acgttatcgt ctgggcggca
     5221 gcactaaaaa aggtatcgat tgttctggtt tcgtacagcg tacattccgt gagcaatttg
     5281 gcttagaact tccgcgttcg acttacgaac agcaggaaat gggtaaatct gtttcccgca
     5341 gtaatttgcg tacgggtgat ttagttctgt tccgtgccgg ttcaacggga cgccatgtcg
     5401 gtatttatat cggcaacaat cagtttgtcc atgcttccac cagcagtggt gttattattt
     5461 ccagcatgaa tgaaccgtac tggaagaagc gttacaacga agcacgccgg gttctcagcc
     5521 gcagctaata aaccgtttgg atgcaatccc ttggctatcc tgacgagtta actgaaagca
     5581 ctgcttaggc agtgcttttt tgttttcatt catcagagaa aatgatgttt ccgcgtcttg
     5641 atccaggcta tagtccggtc attgttatct tttaaatgtt gtcgtaattt caggaaatta
     5701 acggaatcat gttcatacgc gctcccaatt ctggacgtaa gctcctgctt acctgcattg
     5761 tagcaggcgt gatgattgcg atactggtta gctgccttca gtttttagtg gcctggcata
     5821 agcacgaagt caaatacgac acactgatta tcgacgtaca aaagtatctc gatacctatt
     5881 ttgccgacct gaaatccact actgaccggc ttcagccgct gaccttagat acctgccagc
     5941 aggctaaccc cgaactgacc gctcgcgcgg cgtttagcat gaatgtccgt acgtttgtgc
     6001 tggtgaaaga taaaaaaaca ttctgttcat ctgcgactgg tgagatggac attccactca
     6061 atgaattgat tccggcgctc gacattaata aaaacgtcga tatggcgatc ttacccggca
     6121 cgccgatggt gccgaacaaa cccgcaatcg tcatctggta tcgcaaccct ttgctgaaaa
     6181 atagcggtgt gtttgccgct ctgaatctca acctgacgcc ttctctcttt tatagttcac
     6241 ggcaggaaga ttacgatggc gtcgccctca ttattggcaa tactgcgcta tctacctttt
     6301 cttcacgttt gatgaacgtt aatgaattaa ccgacatgcc ggtccgtgaa actaaaattg
     6361 cgggcattcc tctgaccgtt cggctttatg cggatgactg gacatggaac gatgtgtggt
     6421 acgcattttt actgggcggc atgagtggaa ctgtcgttgg cctgctttgc tattacctga
     6481 tgagcgtacg tatgcgcccc gacagagaaa tcatgaccgc catcaagcgc gaacaatttt
     6541 acgtggcgta tcaaccggtg gtggatacac aagctttgcg ggtaacgggc ctggaagtac
     6601 tgctacgctg gcggcatcct gtagcgggag aaattccccc ggatgccttc attaactttg
     6661 ccgaatcgca aaagatgatt gtgccgctga ctcagcacct gtttgagtta attgcccgcg
     6721 atgccgcaga attagaaaaa gtactgccgg taggcgtcaa atttggcatt aacattgcgc
     6781 cggaccatct gcacagcgaa agctttaaag cagatatcca gaaactgctc acttccctgc
     6841 ccgcacacca tttccagatt gtgctggaaa ttaccgagcg cgatatgctg aaagagcaag
     6901 aagccacaca actcttcgcc tggctgcact cggtcggcgt agaaattgct attgatgact
     6961 tcggcaccgg gcacagcgcg cttatctatc ttgagcgttt tacgctcgat tatctgaaaa
     7021 ttgaccgtgg atttatcaac gccatcggta cggaaacgat cacttcaccc gtacttgacg
     7081 cggtgctgac gctggcgaaa cgtctcaata tgctgacagt tactgaaggg gtcgaaacgc
     7141 cggaacaagc gcgatggcta agcgaacgcg gcgttaattt catgcaaggc tactggatta
     7201 gtcacccgtt accgctggac gattttgttc gctggctgaa gaaaccgtat acgccgcagt
     7261 ggtaaggtgt gcttacgtcc cttattattc atagtgaaag catgccggat tgcggctaat
     7321 gatgagtaaa aggaaatccg ttgcagatga ttgtgcgcat actgctgctg tttatcgctc
     7381 tgttcacctt cggtgcgcag gcgcaggcta tcaaggaaag ctataccttt gccgtgctgg
     7441 gcgaaccccg gtacgcgttt aatttcaacc attttgatta tgtgaacccc gccgcgccaa
     7501 aaggtgggca gataacgttg tcagccctcg gtaccttcga taatttcaac cgctatgcac
     7561 tgcgcggcaa cccgggcgca cgcaccgagc agctgtacga cacgttattt acgacttccg
     7621 atgacgaacc aggcagttat tacccgctga ttgctgaaag tgcacgctat gctgacgatt
     7681 attcctgggt ggaggtcgct attaatccgc gcgcccgttt tcatgatggt tctcccatta
     7741 ctgcccgcga tgtagagttt acttttcaaa aatttctgac cgaaggcgtg ccgcaatttc
     7801 gtctggtcta caaaggcacc accgtcaaag ccattgcgcc gttaaccgtg cgcattgagt
     7861 tagctaaacc cggcaaagaa gatatgttga gtctgttttc gctgccggta tttccagaaa
     7921 agtactggaa ggatcacaaa cttagcgacc cgctcgccac gcctccgctt gccagtggtc
     7981 cgtaccacgt tacgtcctgg aaaatggggc aaaatattgt ctattcccgc gtgaaagatt
     8041 actgggcagc aaacttaccg gtaaaccgtg gacgctggaa tttcgacacc attcgctacg
     8101 attattacct cgatgataat gtcgcctttg aagcgtttaa agcaggtgcc tttgatttgc
     8161 gtatggaaaa cgacgctaaa aactgggcca cgcgttatac cggtaaaaat ttcgataaaa
     8221 aatacatcat caaagatgag caaaagaacg aatcagccca ggatacgcgc tggctggcgt
     8281 ttaatatcca acgtgcggta ttcagcgatc gccgggtgcg ggaagcaatc actctcgcct
     8341 ttgactttga atggatgaac aaggcgttgt tttacaacgc ctggagtcgc acgaacagtt
     8401 attttcaaaa taccgaatac gcggccagaa attaccccga cgccgcggag ctggtgcttc
     8461 tggcaccaat gaaaaaagat ctaccgccag aagtcttcac acaaatctac cagccgccgg
     8521 tatctaaagg cgatggctac gatcgtgaca acctgttaaa agccgacaaa cttctcaacg
     8581 aagcgggctg ggtgctgaag ggtcagcaac gcgttaatgc cacaacgggt cagccgctca
     8641 gctttgaatt attgcttccc gcaagcagca atagtcagtg ggtattgccg ttccagcaca
     8701 gcctgcaacg gctgggtatc aatatggaca ttcgcaaggt ggataactcg caaatcacta
     8761 accgcatgcg caatcgcgac tatgacatga tgccgcgact atggcgggcg atgccgtggc
     8821 ccagttccga tttacagatt tcctggtcat cggaatatat caattccact tataatgccc
     8881 ccggcgtgca aagcccggtt atcgactcac tgatcaacca aattattgcc gcgcagggaa
     8941 ataaagaaaa attactgccg ttggggcgag cactggatcg cgtattaacg tggaattatt
     9001 acatgctgcc aatgtggtac atggcggaag accgtctcgc ctggtgggat aaattctccc
     9061 aacccgctgt acgccctgtt tacagcctgg gtatcgatac ctggtggtat gacgttaata
     9121 aagcggccaa actgccgtca gccagacaac agggagagta gatgggcgct tacctgattc
     9181 gccgtctgtt gctggtgatc ccaacgttat gggctattat cactatcaac tttttcatcg
     9241 tgcaaattgc gcctggcggt ccggtcgatc aggccatcgc cgccattgag tttggtaatg
     9301 ccggagtatt acccggcgca ggcggtgaag gtgttcgtgc cagccatgcg caaacgggtg
     9361 tcggcaatat cagcgacagt aattaccgtg gcggacgcgg attagatcca gaagtgatcg
     9421 ctgagatcac tcatcgctac ggtttcgata agccgatcca cgaacgttac ttcaaaatgc
     9481 tctgggacta catccgcttt gattttggtg atagcctgtt tcgcagcgcc tcggtgctga
     9541 cgctgattaa agacagtctg ccggtttcca tcaccctcgg attgtggagc acgctgatta
     9601 tctatctggt gtcgattccg ttaggcattc gcaaagctgt ttataatggg agtcgctttg
     9661 acgtctggag tagcgcattt atcatcatcg gctacgccat tccggccttt ttgtttgcca
     9721 tcctgctgat tgtcttcttc gcgggcggca gctatttcga cctgttccct ctacgcggcc
     9781 tggtttccgc taactttgat tcgctgccgt ggtatcagaa aatcaccgat tatctgtggc
     9841 atatcacgct accggtgctg gcgacagtga ttggtggctt tgcggcgctg accatgctga
     9901 ccaaaaactc attccttgat gaagtgcgca agcaatacgt ggtgaccgcg cgtgcgaaag
     9961 gggtaagtga gaaaaacatt ctctggaaac atatgttccg caacgccatg ctgctggtga
    10021 ttgccggttt tccggcgacg tttatcagca tgttttttac cggctcgctg ctgattgagg
    10081 tgatgttttc actcaatggt cttggcttac tgggctacga agcgaccgtc tcgcgcgatt
    10141 atcctgtaat gtttggtacc ttgtatattt tcaccctgat tggcctgctg ctgaatattg
    10201 tcagtgatat cagctatacg ctggttgatc cgcgtataga ttttgaggga cgttaatgtc
    10261 gcgactcagc cccgtcaatc aggcccgttg ggcgcgtttt cgtcataacc gtcgcggcta
    10321 ctggtcgtta tggattttcc tcgttttgtt tggtttgagt ttgtgttctg aacttatcgc
    10381 taacgataaa ccgttgctgg tgcgttatga cggcagttgg tatttcccgt tgttgaaaaa
    10441 ctacagcgaa agcgattttg gcggcccgct ggcaagtcag gctgattatc aggacccgtg
    10501 gctgaaacaa cggctggaaa ataacggctg ggtactgtgg gcaccgattc gctttggtgc
    10561 taccagtatc aactttgcta ccgataagcc cttcccttct ccaccctccc ggcaaaactg
    10621 gctgggaacg gatgcaaacg gcggcgatgt gctggcacgc attctctatg gcacgcggat
    10681 ctcggttctg tttggcctga tgctgactct ctgttccagc gtgatgggcg tgctggcggg
    10741 ggcgctacaa ggctattacg gcggtaaagt cgatctctgg gggcaacgct ttattgaagt
    10801 atggtcgggg atgccgacgc tgtttttgat tattttactt tccagcgtcg tacagcctaa
    10861 cttctggtgg ctgctggcaa ttactgtctt gtttggctgg atgagtctgg tcggcgtggt
    10921 gcgggcggag tttttacgta ctcgtaattt cgactacatt cgtgcggcac aggcgcttgg
    10981 cgtcagcgat cgcagtatca tcctgcgtca tatgttgcct aatgccatgg tcgcgaccct
    11041 caccttttta ccgtttattt tatgtagttc gataaccacc ctgacctcgc tcgatttcct
    11101 cggcttcggt ctaccgctcg gttcaccgtc actcggtgaa ctgctgttac aagggaaaaa
    11161 taaccttcag gccccgtggc ttgggatcac cgccttcttg tcggtggcga tattattgtc
    11221 tttgctgatc tttattggtg aagccgtccg cgacgcattt gatcctaata aggcggtgta
    11281 gcatgacgca aactctgtta gcgattgaaa atttgtcggt gggttttcgc catcagcaaa
    11341 ccgtacgtac agtagtcaat gatgtttcac tacagattga ggctggcgaa acgctggcgc
    11401 tggtgggtga gtcaggttca ggcaaaagcg ttaccgcgct gtcaatttta cgcctgctcc
    11461 cttccccgcc ggttgaatat ctctccggcg atattcgttt tcatggcgaa tcgctgcttc
    11521 atgccagcga tcaaacgttg cgcggtgtac gcggtaataa gatcgccatg atttttcagg
    11581 aaccgatggt gtcgttaaat ccattgcata ccctggaaaa acagctttat gaagtgcttt
    11641 cactccaccg cgggatgcgt cgggaagcgg ctcgtggcga aattcttaac tgccttgatc
    11701 gcgtcggtat ccgccaggcg gcaaaacggc tgacagatta tccgcatcag ctctccggcg
    11761 gcgaacgcca gcgggtgatg attgcgatgg cgctgttaac gcgaccggaa ttattaattg
    11821 ccgatgaacc gaccaccgca ctggacgtct ctgtccaggc gcagatttta cagctgttgc
    11881 gcgaactgca aggcgagctg aatatgggca tgctgtttat tactcataac ctcagcattg
    11941 tcagaaaact ggcccaccgc gtggcggtaa tgcaaaacgg tcgctgtgtc gagcaaaata
    12001 acgccgctac gctatttgcc tcacccactc atccttacac acaaaagcta ctcaacagtg
    12061 aaccgtcagg cgatccggta ccgttgccag aacctgcctc aacgttgctg gatgttgaac
    12121 agcttcaggt tgccttcccc attcgcaaag ggattttgaa gcgcattgtg gatcataatg
    12181 tggtggtgaa aaacatcagt tttacgctac gggcgggtga aacactgggt ttagtgggcg
    12241 agtctggttc cgggaaaagc acgacgggac tggcgctgct gcgactgatt acctctcagg
    12301 gcagcatcgt ctttgacggt cagccactgc aagatttaaa tcgccgccag ctgttaccta
    12361 ctcgtcatcg tattcaggtg gtatttcagg atccaaactc ctcgctcaac ccacgactca
    12421 acgttttgca gattattgag gaaggcttac gggttcacca gccgacgctt tctgccgcac
    12481 aacgcgaaca acaagtgata gccgtgatgc atgaagtggg attagatcct gaaacacgcc
    12541 accgttatcc ggcggagttc tctggtggtc agcgacaacg tattgcgatt gccagggcat
    12601 taattcttaa gccctcgctg atcatacttg atgaaccgac atcatcactc gacaaaacgg
    12661 tacaggcgca aatattgacg cttttgaaat cattgcaaca aaagcatcaa ctggcctatt
    12721 tgtttatcag ccacgatttg cacgttgtcc gcgcgttatg tcatcaggtt atcgtactgc
    12781 gacaagggga agtagtggaa caaggaccgt gcgcgcgcgt gtttgccaca ccgcagcagg
    12841 agtatacgcg tcagttactg gcgttgagct gacgcttaaa aaggattgta gtttgaaaag
    12901 ggttcggcaa ttgccacacc aaaatttttc aatcggcatg ttgcagcaaa ctcgtcctgg
    12961 cgatttacaa acaggcacgg ctcaccttca cactccaaca ccgaacatgg aacttcgatg
    13021 tcgcttaacg cctggcttag tttatacatc accgaccatg cattatcacc atccgggcta
    13081 agcaatttaa gcgccaccag gctgttgtca ccgcacggtc cattttgcgg aatcggttca
    13141 accttcgaac ctgcgaaacc agcagaccag cgatagttct gcggcagtcg atggacgatt
    13201 gagagttgta atgaagtcac ttattttccc cggaagtaca ttaacttcac aatttgttta
    13261 catcaatttt aacacatcat caacaatccg tctttataca gatcgtaaag attgatgcct
    13321 cgcctgcgtg gcctctatgg ctctgattta agtaatatct gcgggcgtgg tcacgtttgc
    13381 ggggctatat gtacccgttt ctgcgatcta actcaacctt tttaactaca atgatgtgac
    13441 tttttacata aattgatttt acataaaata aacatatatc ggggaaatga tagatttgtg
    13501 gttgacatgc atcaacaaag gaagcctttt accttcctcg ttgtgcaata gatcaccgtt
    13561 ttttcggccg actggcgtac agacagaaga gaatggagct ggttgcgcag aatgcaattg
    13621 accaaatcat cggccatgca gagttaaagg tcgcaagaga aagcaatgcg ccaacaattg
    13681 cccctatgcc aaaacggaag gttcctgcca gcgaagatgc cgttcccgcc atatggggaa
    13741 actcatcaag aatgaccgcc atcgcattgg atgacaccat cgacacgcag cccacaaacg
    13801 ccgcaacgcc aaccaccagc gaccagaatc ccagccccag cggcgcactg atgaccatcc
    13861 acgcagccat aataaattgt atccacaacc ccgagcggaa catatttaac gcgccaatgc
    13921 ggcggacgaa gcggctgtta aagatggtca tcacgaacag aaaaacaatg tttagcgcaa
    13981 agtaataacc aaagttttcc ggcgcgacgt ggttaatttc aatataaaca aacggtccgg
    14041 cgcttaagaa tgagaacatc ccggcaaagc tgaaaccact ggcaagcatg tagctcagga
    14101 cacgtttatg gcggaacagc gccgcaaagt taccaatagt ggtacgaatg tgaaatggct
    14161 gacgacgctc cggtggtaag gtttctttaa tcaggaagaa aatcattgcc gaagccagaa
    14221 tcgccgctaa tgccaggatc cagaagatgt aatgccagct cagccacacc agcacccagc
    14281 cgccaactat cggtgccatc agcagtgcaa tggtcgtcac cagcatgaca aacgacatca
    14341 tccgcgagaa ctcttctttc gggtaaatat cacgcatcaa agcgttgata accacgctgg
    14401 ccgcagccgc agccagcccg tggaagaaac gcatcacaat cagctgatcg atggtttgtg
    14461 ccaacgcaca cgccaccgcg gcggcggcaa acaccagcgt cccgccaaga accaccggct
    14521 tacgcccgaa gctgtctgcc atcggcccgt agattaactg ccccaacgca aagcccagaa
    14581 tataagtact gagggtcatc tgcgtactgc ccgccgatac gccaaactgc gctgaaatta
    14641 ccggtagcgc gggcagatac atatcaatcg acagcggcat caacatggcc agcaggccaa
    14701 ggataaaaac aatagcaaac gacgaatgct gtcgggtggt cacaacgggc tcctgaaagt
    14761 cattgaaaag ttagacgacg ctggcaattt cttcttcagt taacggacga tattcaccgg
    14821 gggctaaatc agcatccagc gtaataccgc caatacgttc acgatgcagc tcaaccacgt
    14881 ggttacccac ggcggcgaac atgcgtttca cctgatgata acgcccttcg ctgatggtca
    14941 gacgaacctg cgttggggta atcacttcaa gcaccgccgg cttagtgaga tctttttcgt
    15001 tatgcagctg cacgccttta gcaaattgct ctgccgtatc gtcagctaca ggtgattcca
    15061 gtgtcaccag ataggtcttc tcgcaatgat ggcgcggaga agtaatgcgg tgcgaccact
    15121 gaccatcatc agtcatcagc accagaccgg tggtatcgat atccagacgt cctgcggcgt
    15181 gcagtttcca cgctaccggt tcatccagaa aatagagcac cgtcgggtga tccgggtcgt
    15241 ccgtggagca aacatagccc tgaggcttat tgagcatgaa gtaacgtgga ccgtgttgct
    15301 gcgccagcgg gttgccatcg taagcgacat catgttcagg gagcagtttg aacgctgcat
    15361 tacgaacgat ttcgccatcg acggtgacac gattgccgcg gatttcacgc ccggcaatag
    15421 cacggctaac gccgagttgc tgtgcgataa atttatcaag tcgcatgtgt gtgattttgc
    15481 ctgtaaaaaa cggaggtcgg gtaatgaccc gaaaatctga actgttgtct gcccagtata
    15541 gcggtctaat aacttccctc aagggaaaaa gaaccgtggc atactattag cagaataatc
    15601 tacctacgcg aaaccatgat ttttacactc cgcccatatc agcaagaagc cgtggatgcc
    15661 acgctcaacc attttcgtcg tcataaaacc cctgccgtta tcgtgctgcc caccggcgca
    15721 ggtaaaagcc tggtgatagc ggaactggca cggctggcgc gtggtcgcgt actggtgctg
    15781 gcacacgtta aagaactggt ggcgcaaaac catgcaaagt atcaggcgct ggggctggaa
    15841 gccgatattt ttgccgccgg gctaaagcgc aaagagagcc acggtaaagt ggtatttggc
    15901 agcgttcagt cggtcgcccg taatcttgat gcctttcagg gtgaattttc gctgttgatt
    15961 gtcgatgaat gccaccgtat tggtgacgat gaagagagcc agtatcagca aatcctcact
    16021 cacctgacta aagtgaatcc ccacttacgc ctgctggggc tgactgccac gccttttcga
    16081 ttgggcaaag gctggatcta ccagttccat tatcacggca tggtacgcgg cgatgagaaa
    16141 gcccttttcc gtgactgcat ttatgagctg ccgctgcgtt atatgattaa acacggctat
    16201 ctgacgccgc cagaacgact ggatatgcca gtagtgcaat acgatttcag ccgcttgcag
    16261 gcacagagta acgggctgtt cagcgaagct gatctcaacc gtgagctgaa aaaacagcaa
    16321 cgcattacgc cacatatcat cagccagatc atggagttcg ccgaaaagcg caaaggggta
    16381 atgattttcg ccgccaccgt tgaacacgca aaagagattg tgggattact gcctgccgaa
    16441 gatgctgcac tgattactgg cgacaacccc ggcgctgagc gcgatgtgtt aattgaagat
    16501 tttaaagccc agcgttttcg ctatctggtc aacgtcgcag tactgaccac cggatttgac
    16561 gccccgcacg tcgatcttat cgccattctg cgccctaccg aatcagtgag tctttaccaa
    16621 caaattgtcg ggcgcggtct gcgtctcgct ccgggcaaga ctgattgttt aattcttgat
    16681 tatgcgggta atcctcacga tctctacgcg ccggaagttg gtacaccgaa aggcaaaagt
    16741 gacaacgttc cggtacaggt tttctgccct gcctgcggtt ttgccaacac cttttggggg
    16801 aaaacgaccg ccgacgggac attgattgaa cactttggtc gccgctgtca gggatggttt
    16861 gaagatgacg acggtcatcg cgaacagtgt gacttccgtt tccgttttaa aaattgcccg
    16921 caatgtaacg cagaaaatga tattgccgct cgccgctgcc gcgaatgtga cacggtactg
    16981 gttgatccgg atgatatgtt aaaagcggcg ctacgactga aagatgcgct ggtattacgc
    17041 tgtagcggca tgtctttgca gcatgggcac gacgagaaag gcgaatggtt gaaaatcacc
    17101 tattacgatg aagacggcgc ggatgtgagt gagcgtttcc gtctgcaaac gcccgcccag
    17161 cgtaccgcct tcgagcagct ttttatccgc ccgcatacgc gcacaccggg catccctctg
    17221 cgctggatca ccgccgccga tatcctcgcc cagcaagcct tactgcgaca tccggatttt
    17281 gtcgtcgccc gcatgaaagg tcagtactgg caagtgcgtg aaaaagtgtt cgattacgaa
    17341 ggtcgttttc gtcgggcgca cgaattacgc ggttaatggt agttttgatt gatgtataag
    17401 gcgattgagt atagaatctc gcccgctttt gcatacgcaa agcagatcac ttacctgttg
    17461 ctgggtcgcc tgtagcagga ttaatttaag agagaaagaa atgtttacta tcaacgcaga
    17521 agtacgtaaa gagcagggta agggtgcgag ccgccgcctg cgtgccgcta acaagttccc
    17581 ggcaatcatc tacggtggca aagaagcacc gctggctatc gagctggatc acgacaaagt
    17641 catgaacatg caagctaaag ctgaattcta cagcgaagtt ctaaccatcg ttgttgacgg
    17701 taaagaaatc aaagttaaag ctcaggacgt acagcgtcac ccgtacaaac cgaagctgca
    17761 gcacatcgac ttcgttcgcg cttaattgct gaataagttg taaaaaaccc cgctccggcg
    17821 gggttttttg tatctgcaga ttatgcctga ttacggtatt gctatttttt gcaggccaga
    17881 taaggcgttc acgccgcatc tggcattcag aacacaaaac cgattaattg ccgccagacg
    17941 tccggcgctg caattggtcg cgcaaattcg gcggcgtgcc tttaatggtc aaggtatcgg
    18001 ttgctggatc ccagaagatg cgttcaccta acagcatcgc atcaaagtta atagtcaagc
    18061 caccgccgct accggcaaac ttcgtcaact gacgcagtgt gctacggtct gccgggaagc
    18121 tctcttccag ttcgtagcct ttttcagccg caaactccgt gaagctcact tcgcttacgc
    18181 ctgccagctc tttcgacagc gattccagtt cgatctcttc gcctgcctgc agctgctcat
    18241 tacagtagct ataaacctgc tggcgcacgt tctgtcgctc tgctttatcc agttgtgctt
    18301 cggcagtgaa atcatcaacc gcttgcaaca gcccacggtt ttgcgctttc gcgttcaatc
    18361 cttcgctggc cccgaggaaa tccataaaga aatccgccac tttgcgccct actcgccctt
    18421 tcaggaaagt gagatagcgg gtggactctg gattggtttc ccattcggtt aaatcgatac
    18481 gcgcaacaat atccgcatga ttgatatcaa gataatgggt tgggttgatg tcgagatttt
    18541 cgttaacgcg catactgctc aggttgctca gaactgccac cagcaaatac tccaccgcca
    18601 gataacggta gtggcaaaac agcacgaatc caccgtcagc aaaaggatat ttcgctaatt
    18661 cgtcacgcag gcgaccggtt gccgcgcggc taaatgccag aaagtcctct tcgccctgac
    18721 gctgtaagcg cagcgtctgc gccagttcac tctcttcgct aaacaagccg taagctttat
    18781 ttttggcgct atagacccga tgcagttctg ccaccatctc aacgacggtt tctgtcggtt
    18841 ccagcaatga atcgcgcaac accagctcaa ggttctgctc atcacgcttg ataagctggt
    18901 gcagagcaat ctggttgata tccagactca tgataaactc tcctttaaga ccgggcggta
    18961 ttcaaccacc gccagttaag cgacgcaata aaagatggcg gtcattttac agaaggcagc
    19021 gtaacgagaa tgatttaacg cataaaaaag cagaaaaaaa accgttgcta cggtaatatg
    19081 ttgccctttc atcaactaac tgatttcgat ttatgccaca aatttcccgc tacagtaatg
    19141 aacaggttga acaattgctc gcagagctgc tcaacgtact ggaaaaacat aaggctccga
    19201 ccgacctttc cctgatggtg ttaggcaaca tggtcactaa ccttatcaac accagcattg
    19261 ccccggccca acgccaggca attgccaact cttttgccag cgccttacag tcctctatca
    19321 acgaagacaa agcgcactaa gggaaacaga taacaggtta tggtaactca tcgtcagcgc
    19381 taccgtgaaa aagtctccca gatggtcagt tgggggcact ggtttgcact gttcaatatt
    19441 ctgctttcgc tcgtcattgg cagccgttac ctgtttatcg ccgactggcc gacaacgctt
    19501 gctggtcgca tttattccta cgtaagcatt atcggccatt tcagcttcct agtgttcgcc
    19561 acctacttgc tgatcctctt cccgctgacc tttatcgtcg gctcccagag gctgatgagg
    19621 tttttgtccg tcattctggc gacggcggga atgacgctat tactgatcga tagcgaagtc
    19681 tttactcgtt tccatctcca tcttaatccc atcgtctggc agttggttat caacccagac
    19741 gaaaatgaga tggcgcgcga ctggcagctg atgttcatca gcgtgccggt tattttattg
    19801 cttgaactgg tgtttgcgac gtggagctgg caaaagctgc gcagcctgac gcgtcgtcga
    19861 cgcttcgcgc gcccgctggc cgcattctta tttatcgcct ttatcgcctc gcatgtggtg
    19921 tatatctggg ccgatgccaa cttctatcgc ccgatcacca tgcagcgcgc taacctgccg
    19981 ctttcgtacc cgatgacggc gcgacgtttc cttgagaagc atggcctgct tgatgcgcag
    20041 gagtatcaac gccgtcttat tgagcaaggt aatccagacg ccgtttccgt acagtatccg
    20101 ttaagcgaac tgcgctatcg cgatatgggc accgggcaga atgtgctgtt gattactgtc
    20161 gatggcctga actactcacg cttcgagaag cagatgcctg cgctggcagg ttttgctgag
    20221 caaaatattt cgttcacgcg ccatatgagc tccggcaaca ctacagacaa cggcatcttt
    20281 ggcctgttct atggcatctc gccgagctat atggacggca ttctgtcgac ccgtacgcct
    20341 gcggcattaa ttactgcgct taatcagcaa ggctatcagc tggggttatt ctcatcagat
    20401 ggctttacca gcccgctgta tcgccaggca ttgttgtcag atttctcgat gccgagcgta
    20461 cgcacccaat ctgacgatca gaccgccacg cagtggatca actggctggg ccgctacgca
    20521 caagaagata accgctggtt ctcgtgggtt tctttcaatg gcactaacat tgacgacagc
    20581 aatcagcagg catttgcacg gaaatatagc cgggcggcag gcaatgtcga tgaccagatc
    20641 aaacgcgtgc tcaatgcact gcgtgattct ggcaaactgg acaatacggt ggtgattatc
    20701 actgccggtc ggggtattcc actgagcgaa gaggaagaaa cctttgactg gtcccacggt
    20761 catctgcagg tgccattagt gattcactgg ccaggcacgc cggcgcagcg tattaatgct
    20821 ctgactgatc ataccgatct gatgacgacg ctgatgcaac gcctgctgca tgtcagcaca
    20881 cctgccagcg aatattcgca aggtcaggat ttgttcaacc ctcaacgccg tcattactgg
    20941 gttactgctg cggataacga tacgctggca attaccaccc cgaaaaagac gctggtgctg
    21001 aacaataacg gtaaataccg cacttacaac ttacgtggtg aaagagtgaa agatgaaaaa
    21061 ccacagttaa gtttgttatt gcaagtgctg acagacgaga agcgttttat cgctaactga
    21121 ttaattataa atcagttagc gaaatatctt acttgcaatc ggtgtggaaa acggtagtat
    21181 tagcagccac gagtcggcac gtagcgcagc ctggtagcgc accgtcatgg ggtgtcgggg
    21241 gtcggaggtt caaatcctct cgtgccgacc aaaaatccca agaaaaaacc aacccttacg
    21301 gttggttttt ttatatctgc aattaattcg ataaacagac cgtgacacat cacagcctgt
    21361 ttattttctg ttatcagaac gtccagacca cacccgcctg agtattccac ggcgattcta
    21421 cccctgcgcc attgccatag ctgactgaca aatgaccgct taacgtcggg gtaaacgatg
    21481 accttatccc agcctgataa acgccactgg ttccagccac atcattatta aatttgccat
    21541 cgtcattaac tttcacctgg ttagaatcgg cgtattcctg acgcacggcg gctttcagcc
    21601 agggttccag cgtcgtaccg ttttgcaggt ccatgtgata gcttaccgcc gttcccgctt
    21661 cagcgcgtaa tatccgggta tttcccacat ccgcgcgcat gccgtttgat aacgtgtagt
    21721 cctgaccatc tgtggtaaag ccggtaaagg ccagataggg tctaacactc cacaatccgt
    21781 caacccaacg gaacccgctc tcaacatgag cacccgcgcc gttactattg taatcgccaa
    21841 acgctgttgc cccattactc atcttgccat ggatggtgtt ggcaaaacgg tcaactttca
    21901 ccaccccatc aacataggca ccgttctgat gctcccaacc ggcataagcc cccagggtat
    21961 agctatcgat attacctttt ccgccgcgat caaaaccaat atcagaatga gagtaaccaa
    22021 agaacaagcc gcgaattgta ctgctttctt cacgggagaa acggctatcg ataccgagcg
    22081 tcaggcccgt caatgtttgc tcaaaaccag ctcccgcatc agtggtcacg ttgttgcggg
    22141 tgttaattgc cgaactccac atcaccgtat cgtaactaac gccttttacg ctaccaagac
    22201 gctcacgcac ggtgtccagt tctgcatcaa ataccagcgg ttgtgcggct gccatgttca
    22261 gcacatcagt ggttgaaggg gtaatttgcg cgcgattctc tgcgaggctc cagctatggt
    22321 tgccattatc cagcaaggta tattcatacg taccgatatc aacaacgcct ccggcattgc
    22381 ccaacgtaaa tgcagcatca ccgccgcccg ttgttaccag tgtaaggcta tctcctgctg
    22441 ccgggctggc accggtgtcc gtcacgaata ttttgaaatc acctgttgcc tgaccggtga
    22501 cgttgagctg atcgctctga tgattggcca tatcggtacg catataaaaa ttgccgtttc
    22561 ccgacagggt attggtggtc aacgtaatga aattttccgc acttgccgtc acagatgaac
    22621 gagtcactgg ctcagccatc ataatcacat ttgcgccgga catcgcaagg ttggctaagt
    22681 tgaggcgagt gttttggttc gtcgtagtga tatcggcaat gatggtccat gcgctatttt
    22741 cgagcgaaac gtccgctttg ctggtatcca cggcaccatg cgttctaaaa gaagcgccct
    22801 cagaaaccac taccttaccc accaacatag cgggtgcata atttatttgc ggcttcatga
    22861 cctcaagaat gccatcattc cctctgactg acacgttaac cattgtgcca gcccagacat
    22921 tagcgcggcc attgttgatt accatatttt cagagatggc ttttgaggaa tacgtgatac
    22981 ttccattctg gtctgatcga ccgagatcat ataccgcatt tgcctgcact ttcgtaccag
    23041 tatcctttcc cagcgattgc atagtggcat gctcatcaag gatagtgtca atggcctgcg
    23101 tgtcctccat aacaatgagc ccggaaccat catccagttc ataatttttt gacacaccat
    23161 cctttatact aaattggcct ttactgtttg taccactcac ttccagcgca ttcgttgaga
    23221 caattaattt cccgcctgct tttttatcaa cgccagttgc agtcccgcca tccataacct
    23281 ccagtaagcc gccactatcc acagtagtat tataagcgaa gtcattttct tcaactcgca
    23341 aacttccgcc gttttccaac aacatattat tagcaatacc attcttgata tcgaactgac
    23401 cgagacggtt tgttccgaat acctccatgg cccgcgtggt tgttttaata gcaccgcctg
    23461 ttttctgatc taccgcaagc gcaaatcccc cctctttaac ctccagtagt cctccctttt
    23521 cgatagcggc aaaaacggcc cccccatctt tttcaacgat taagcgcccg cctttaatcg
    23581 tggtatcatt cgctgggcca taaacctgta gcacaccatt attgagggta gtctggtttg
    23641 ccgcgccacc catttgaaca atttgataac catcatttaa tactgagtat tctgcggtac
    23701 cattcatttc aatgtattga taccctccgt taatttcagt attactactt acaccaaatt
    23761 cttttatatg ctgttctccg ccaggattga ttttcgtatt attggcggtc ccataaacca
    23821 gctggatatc tttttccagg ataacaccat caactgtctc accatactca actgttgatg
    23881 ccaatgctat gggtgaatat aacgctgtag ccaccagaac agatattgcg gaacgacaaa
    23941 gagaaacaga accagattga tgcattgagc tttcatccta tgaaattaat tgcagttaaa
    24001 agcattgggt acagaaaata cccatagctc catacccgga gtcagttttt aaaaactgtt
    24061 taaagaaatg cacaagtatt gtgattgatt ttttagttgt ttttcttgat gagaagctga
    24121 tgcaaaattc cgtctttata ataaaaatga tgccaaagcg aacgacaagg ttgtagtttt
    24181 cactacatgt ccatacataa aatggggtaa cattcacgcg cctggtagcg ttaccaacgc
    24241 tacgctcaaa cataatgatt ctaataaaac ctcaggagac tactatgcct gaagcaacgc
    24301 cttttcaggt gatgattgtg gatgatcatc cacttatgcg acgcggtgtt cgtcagttac
    24361 tggagcttga tcctggcttt gaagtggtcg ccgaagcggg cgaaggcgcg agcgcgatcg
    24421 atctggcgaa tagactggat atcgacgtga tcttgctgga tctcaatatg aaaggtatga
    24481 gtggcctgga taccctcaat gccttgcgca gagatggcgt taccgcgcaa attattatcc
    24541 tgaccgtatc cgatgcctcc agcgatgtct ttgcgctgat agacgcaggc gcagacggtt
    24601 atctgttgaa agacagcgac ccggaagtat tgctggaagc gattcgtacc ggagcgaaag
    24661 gcagcaaagt ctttagcgaa cgcgtcaatc agtacttacg tgaacgtgaa atgtttggcg
    24721 cggaagaaga tcccttcagc gtgctgacgg agcgcgagct ggatgttctg cacgagctgg
    24781 cacaggggct gtcaaataaa cagattgcat cggtgttgaa tatttccgag cagacagtaa
    24841 aagtacatat tcgcaatctg ctgcgtaaac tcaatgtccg ctcacgcgtg gcggccacca
    24901 ttctgttcct gcaacaacgc ggggcacaat aaaaatagcc cgatggattt atcatcgggc
    24961 tgagatttat gacaaacgca aaactgcctg atgcgctacg cttatcaggc ctacgtggat
    25021 cgatcaattt attgaattta cacaattttg taggccggat aaggcgttca cgccgcatcc
    25081 ggcataaact aagcgcactt tgtcatcagt ctagcccgat ggcatcacca tcgggcctct
    25141 ttttatttac tctcctgcgg cgacaaatgt tgcatcgcct gcgcgatact acgttcaatc
    25201 accgcacggc gagtatcgtt ggcaggtaag agtttcaaca tcatctccca cgcggcaacg
    25261 gcttcgccaa atcgctgctg ctcaaaggca ttaaacgcat acatgcttag cacacggata
    25321 ttgctatggt ccgttctcac cagctgacgt agcagttcac cgccgaggcg gttgtcgttg
    25381 ggatcagatg aacgagtcaa cgcttcagcg tatcccagtg cggcatcgct gttcttcgga
    25441 tcgaggcgat acgcagtagc gtatgcatcg gtggcgatac tggcgttacc cagcgccatg
    25501 ccaacgcggc ccaacataat ccagccttct atatctcccg gatttttttg cagttgagta
    25561 cgcatcccca gcgcaaggcg cgacatctct tcttcgttga gcggatcggc tttcgggtcc
    25621 agcgccctgt ccagcaacgc cggagcctgt gccgtggcct gctgccagat tttcacctgc
    25681 tgataattgc cagtctggta gtagctgacg ccagccacaa ttaacgccac cacaataccc
    25741 ggcagataaa caacatatcc ggcacgctta ccttccggca cgctttgttc aggaaacgct
    25801 tccggcacca cgcgtacccg acgccgcgag cgggcgtata tgacccaacc gccaatgcca
    25861 atagccacta ctggcagcac ccacagcagc acggtcagcg gcgttaacgg cggatcgtaa
    25921 gtgacgaagt tgccgtaacg cgccaccata taatcgacaa tctctttttt acttttacct
    25981 tcctgcatca gttcatacac tttctgacgc aggtcggtgg caatcatcga gttggaatcg
    26041 gcaatgctgt tgttctggca tttcgggcag cgcagttctt cagtgagctg acggaactgt
    26101 tgttcctgtg cttcatcttt aaactgcaac acgtcgatgg tcgccagcgc tgagccggag
    26161 atcatcagca tcagcacgcc caataaaaac ctcattgtgc ggcctcctta ctgtatttct
    26221 cccacagcgg cttgatctct tcttcccaga cgcgaggatt gagatcgccc gcatggcgat
    26281 agcgaatgat accgttgccg tcaataagga aggtttctgg cgcgccatag acaccgagat
    26341 ccagccctaa catgccgtcg ccatcaaaca ggcttagcgc gtaaggattg cccagctctt
    26401 tcagccagct gattgccttc tggcgatcgt ctttatagtt catgccgacc acgcgaatgc
    26461 cctgcgctga aagctgattc agatattgat gttccgcacg gcaggtcgga caccaagtcg
    26521 cccagacgtt aagcagtact ggtttgccct gagtcagcac atccgcctga taaaactgcc
    26581 ccggattgtc cagtgattcg agacgaaact tcggcacagg cttgccaatg agcgccgatt
    26641 ccagattggt cggatcatcc ccttcggcat tacgcgccag ctgccacagc agcgccgcgg
    26701 caatcgccag gaagataatc aacggaatta acaatacttt gcgcttcata cggcctccgg
    26761 cgcagttttt tgcggactca cgcgcttacg atagcgagga tcaaacagac acagcagtcc
    26821 gcccaacgcc atcatcagcc cgcccgccca aatccagcga acaaatggtt tgtagtaaag
    26881 acgcacggcc cacgcgccgt tttccagctc ttcaccgagc gcggcgtaca ggtcacgcgt
    26941 gatgccgccg tcaattgccg cttcggtcat catcgacccg gcagtgttgt aataacgttt
    27001 ttccgcatac agcaccgttt ccggtttacc ctctcgcgtt acgccaatag tcgccacacc
    27061 gccgcgccag ttcgggccag tcacctcttt gacatcacgg aaggtgaagc gatattcatg
    27121 aatatcgacg ctatcgccgg acttcatgcg cacatcacgc tcaacgctat aattctggct
    27181 aaaggcaatg ccaacaattg tcactgccag ccccaggtga gccgccacca tcccccaata
    27241 actgaaggtg gttttcgtgc cgcgtgaaat acgcagcgca gcttccgcag ttgccagcac
    27301 cgcaatccag caggccattg ccaggccgag caccgtcatc gccacaactt tgctttcgaa
    27361 cagccacggc aacagcagag acagcaccag cgtagagatg aaggcgataa tcaataaatt
    27421 gcggatttta cgtgggcgat cgcgccccca gcgcaccaga ggaccgacac caagcagtag
    27481 cgcaaacggc accatcagcc aggtaaacat ggtgttgaag aacggttcgc caatcgaaat
    27541 actgcccagt ccaagttgct tgtgcaccag cggcagcagc gtccccagca acaccaccag
    27601 catcgcggcg accagcaaaa cgttgttcgc taacagcaaa gattcccgcg accacagcgc
    27661 attgtttacg cgtgagcgaa ctttgtgtcc acgcgcggca aacagcagca gcgaaccgcc
    27721 aatcaccagc accataaagg cgaggataaa cataccgcgc gccggatcag acgcaaacgc
    27781 gtgtaccgat accagcacgc cggaacgcac gaggaaagtc cccaacagac acaacgagaa
    27841 ggcactgatt gccagcagta atgtccacgc tttgaagctg gcgcgttgtt cagtgaccgc
    27901 cagtgagtgc atcagtgcag tccccaccag ccacggcata aacgaggcgt tttctaccgg
    27961 atcccagaac caccagccac cccagccgag ttcgtaatag gcccatgcgg aaccgagcac
    28021 aatgccgagc gttaggaaaa tccacgccgc cagcgtccac gggcgagtaa aacgcgcata
    28081 agtgctgtcc agacgcccgc tcagcaaaga agcaatggca aaagcaaacg ccaccgagaa
    28141 acccacgtac cccatataaa gcagaggcgg atggaagatc agccccggat cctgtaacag
    28201 cgggttaaga tcgcgccctt caatcgggaa gttcggcagc gtgcgtgaga acgggttaga
    28261 ggtaaagaga atgaacagca aaaagccgac gctgaccatc cccattatcg ccagtacacg
    28321 ggccacaata tccagcggaa tacgctgact aaaaatcgcc accgcaaagg tccagccgct
    28381 catcagcagc acccacagca gaagcgaacc ttcatgcgcg ccccaagtag ccgccacgcg
    28441 ataccacacc ggaagctggg tattggagtt gctggcaaca taggtgacgg taaagtcgtt
    28501 gaccacgaaa gcattgacca gcaccagaaa tgcgccagcc acagacataa acagcagcca
    28561 ggcaaacaag cgggaagacg ccatcatgcg cgcatctccg cgcgccacgc cccatagcgg
    28621 atacacggac agcagcagcg caatccctaa cgccaagcac agcagcccgt ttccaatttc
    28681 cggcatcatg atgctgggtc cttataaaca ctcgccgggc gacggtggtt agcttccatc
    28741 gctttctcaa cttctggcgg cgtgtagttt tcgtcgtgtt tcgccagcac ttctttcgcg
    28801 aggatatgat tgcctttttc cagctcgccc tgcaccacaa cgccctgccc ttcacggaac
    28861 agatccggca aaatgccttc gtaagagaca tccactgagc cttcagcatc gtaaatggtg
    28921 aaggtcactt tcagcgaatt gggatcgcgc tgcacgctac ccggcatcac catcccgcca
    28981 acgcgcagac gctgaccgac ttccggcatc tgctgagttt cacgcttgcc gtagagaatt
    29041 tcccccggcg tataaaagag atcgatattc gagcgcagcg catatagcac cagaccgata
    29101 gtcagcgcta gccctgccaa cacggcacag gcaatccaca agcggttttt acggcgaata
    29161 ttcatgcagc ctcctgctgt tgcgcagcac gtaaacgcgc ctcacgcgcc cgctgttgcg
    29221 ccacaccacg cagaattgcg cgatgttgca tcaccgagtg cacgaccaaa accaccagcg
    29281 gaataacggt catcaccacc gccagccaga caaaaaaggc gtaaccgccc attgcgaaaa
    29341 attcattcca ggaagcaaat gcaggggtca tttacggcct cttttcagta tcagttcact
    29401 cacccacgga cggcgttttt ccatcagcaa aatcaaatta cgcatccgca tcagcgtcag
    29461 cgtggcagac aggagcagga agccaaaaat cgaccagcgc agcggcgaac gcatcgccgg
    29521 atcgatactt tgctgcatcc gcgttgatcc ctgatgcagg gtgttccacc actccacgga
    29581 gtaatgaata atcggcagat tcaccacgcc aatcagcacc aggatacctg ccgcacggcc
    29641 cgccagacgg cggtcgtcga aggcgtgcca cagggcaatc acacccacat acaaaaacag
    29701 cagcaccagt tcagaagtca gacgtgcatc ccatacccac caggtgcccc acatcggttt
    29761 tccccatgca gagccggtaa ccagggcaat aaaggtaaac acggcaccaa tgggggccat
    29821 cgccgccacc gccaggttgg ccattttcat ctgccagaca aggccaataa acgccgccac
    29881 cgccattgat gcataaatgc ccatcgacca gatcgccgca ggcacatgca ggtagataat
    29941 gcggtagcta tttccctgct gataatcagc cggagcaaag ccgaatcccc agatccagcc
    30001 gacggtaagc acgaccacac tggcaattgc cagccacggt ataaaccagc cacagatttg
    30061 atacagccgt ggtgggatcg ccagttgatg cagtgttttc cacatagttt cgataccaga
    30121 ctcgaacaaa aatcagtaat ccagcgttat tgaatgctga ttcgtaacgc tgccgccgtc
    30181 gcaaaaggac ttaatgtcgc ggtgcctgcc agcaacgcgc ctaaaattgc cagataccca
    30241 tcaacgggca aatgcataga agccgcgtcc atcgcggcgg tggcaaagat gagtaatggg
    30301 atagtcagcg gtaacaccag tatgctgagc agcacaccac cgcgcttaag tcccactgtc
    30361 agcgccacgc ccggtgcacc gagaaagcca agcgtaggcg ttcccagcag cagcgtcagc
    30421 gccatcactt gccagccata aacatccatt cccagtagca ttgctaccag tggcgaaagg
    30481 atgagtaacg gcagaccggt taccatccag tgcgccatca ccttcgccag cacaacggcg
    30541 ggcaagggta acggcaacaa catcaattgt tcaagactgc cgtcctgcaa atcgtcacgg
    30601 aacagtcgtt ccagcgccag caaggatgaa agcagcgcag caacccagat aatgcccggt
    30661 gcaatacgcg ccagcagttg cggctccgga ccgatactga gcggaaaaag ggtaattaca
    30721 atcaggaaga accacagcgg gttggcgatt tcggcgctat ggcgaaacgc tacgcgcagt
    30781 tcaagacgga aaatacgcca gaacatcatg cggccctcgt ttgcgtcagt gaaatgcggc
    30841 gaattttact ttcagcaacg ttgagcggct ggtgggtagt cagaatcaca atccccccct
    30901 gctccgtatg ctgcgccata cgctgggtca gacgatcgac gccgttaacg tcaatcgcgg
    30961 taaaaggctc gtcgaggatc cataatgtgg cacgggtcaa ccacagacgc gctaaagcga
    31021 cgcggcgttg ttgcccggca gaaagttgat ttaccggaat atcttcgaat ccggcaagcc
    31081 cggcctgcgc caaggcttcc agacattgtg cggcatcgcc atcgcgatga taaaagtgca
    31141 gattttctaa cgccgtcagc cgggttttga tccccggctg atggcctatc cataacaggg
    31201 tttgatggta gctgtcgcgt acctgatgca agggctgccc ttgccagaga acctcgccag
    31261 cgtcagggcg agacaacccc gtcagcaaac ggagaagcgt tgtcttcccc gcgccgttgc
    31321 taccggtgat ttgtacccac tctcctgcgt tcagcgtaaa tgacaagcca ctaaataagg
    31381 ttcgttcatc ccgctcacaa agtaactctc tggcttcaag catacccacg caataaccct
    31441 gttaaaaacc tggctcgact tcacgcatat cgggcagctt gtgcgcgatc cctttatggc
    31501 aatcaataca ggtttgccca tctttcaccg cctggtcatg catcttcgcg gcaaccgatt
    31561 tctgggcggt tgtatccata tactcgaagt tgtgacagtt acggcactcc tgcgagttat
    31621 tgtccttcat gcgccgccac tcattctgtg ccatcgtcag acgatgagct tcaaatttct
    31681 gcggcgtgtc aataacgcca aaaattttac catacagctc tttacttgct ttgagcttgc
    31741 gtatcatctt cggcacaaac tcgtgcggaa cgtgacaatc cggacaggtc gcacggacgc
    31801 cgctacggtt gttgtagtgc acggaatcca tgtattcctg atacaccgtg ttgcgcattt
    31861 cgtggcagct aatgcagaac tcttcggtat tggctttttc catcccggtg ttaaagcccc
    31921 cccagaagac gatgccgcca acaaaaccga tcaacagcag cgtccccagc gccagatggc
    31981 tgggggtacg ccaccatttc cacaggcgct taatcagacc aggcttacgg tcagaatttc
    32041 ccataataac ctcttatttc ccgtaacctt ttgatggggt aaaggtattc cccacgattg
    32101 gcgcggtatc ggcctgcggt acgtgacatt gcagacagaa ataacgacgc ggagccactt
    32161 ccgcgcccac tttgccgtcg ctgtccataa agtgagtagg actgatacgc ggcgcgccag
    32221 tggtgcgata gctttcgaca ccgtggcact gcaagcagcg attggtattg gtcgttacct
    32281 gataaccttc aacgctatgc gggatcattg gcggctgatt cacatagttc agcggcatcc
    32341 gatcctgctc ttttggcatc cgaatggccc cttcctgcgt cccggatact tccggcgact
    32401 ggctaaaatc gacgccatta gccgcccaaa ccgcgccgct taccaccagg gccagcatcg
    32461 ccgtccattg acacagcgct ttcttcaggt catggctttt catgatttcg ctcccgaact
    32521 ccatcgtgta gttattgtaa atacatcctc agaacagaca tccacgcagc gaccgcacgt
    32581 catgcaatcg cggctggtga cctgcaccgg gctttgttca tccagcaccg gggcacgtag
    32641 cacatgcggt tccgggcaaa catgaaaaca atccatacag cggttacatt tctgccgatc
    32701 gctggccgca acggtaatca cacctttgct acccagcacg ccatacagcg cgccaaccgg
    32761 acaaatgtgc ccgcaccagc cgtgttcaac gaccagtaga tcaaataaaa acagcgcgag
    32821 aataagcagc gcgccgctgc cgaagcccat taccaggcta cgccccatca gagaaacggg
    32881 gttgatccat tcccaaatga gcgtgccagt taaggctgat cccaccagaa tgaccaccaa
    32941 cagcacgtag cgaatgtggc gagggatcgt cgcagactga ttgaggtcaa agcgcctgcg
    33001 taaccagttc gctaagtcgg taatcgggtt cagcgggcag acccagctgc aaaataatcg
    33061 ctttcccgcc agggcgtaga gcacggtgat aatcaccgca ccggtcaacg ccaccgtggc
    33121 aggcagatga ccactggcaa gactttgcag cgtcatcagc ggatcggtca gcggaacggt
    33181 gtcgaataac aggctactgc tgtagttgcc gtgcaagatc cacacaccaa accatggacc
    33241 gctcaaaaac atccccagca cgaagaactg acaaaggcga cgtaacacca gccaacggtg
    33301 actgcgccac cagccttttt tctccagcgc ctcgcgcccg gcgtcacgtt tacgatttgc
    33361 cattgttccc ctccagccag ccgaagcggt aatggtgacc taactcccct ttcgccagtg
    33421 acagcggtaa caccttgatt gccggttgtt ccagcacgca tactttttcg cacttaccgc
    33481 aaccggtaca ggcgtcgctg tgaaccgtcg gcagaaagcg ggcgtgctta ccggtacgcg
    33541 tgttgcgctc cagctccaag gtgatggcct catcaatttt cgggcattcg cgataacaaa
    33601 catcgcagcg cagcccctga aagttgagac agttttcctg gtccaccagt accgccagcc
    33661 ccatccgcgc gtcgtcgatc gattcaatct cacgatccag cgcaccgctt gggcacactt
    33721 tggcgcacgg aatgtcctca cacatttcgc aaggaatatc ccgtgcgaca aaatatggcg
    33781 tgcccgccga cagaccagag gccagcgtcg ccagttttaa ggtgtcgtaa gggcaagcct
    33841 gaacacactg accgcaacgc acacaggcac tggcaaaggc gttctcgttt atggctccgg
    33901 gcgggcgcaa ccgcacgcca gatgcgcgtg cggtttgctg ttgtaacccc agcgccacac
    33961 ccacggcagc cagcccgcct gctgtgcgaa cgacatcgcg cagaaagcgg cggcgaccat
    34021 tttgaggttt cgctgaccgg gacataatgg cttacacctt ctccagtttg accgcgcact
    34081 tcttgaagtc cgtctctttc gagagcggat cggtcgcatc cagcgtcagt ttgttaacca
    34141 gctgtgcggc gtcgaagaac ggcatgtaca ccaggccctg tggcggacgg ttacgaccgc
    34201 gcgtttcaac aatcgagatc acttcgccac ggcgagaaac cactttcact ttgtcgccac
    34261 ggcgcagatc gcgcgctttc gcatccagcg ggtgaataaa caagaccgct tccgggaagg
    34321 cgcggtgcag ttccggcaca cggcgagtca tactgccggt gtgccagtgc tccagaacgc
    34381 gtccggtaga gagccacagg tcgtactctt catccggtgc ttccgccgcc ggttcgaacg
    34441 gcagtgcgaa gatcaccgct ttgccatccg gtttaccgta gaacttatag ccttcgcccg
    34501 ctttcacgta cgggtcgtta ccttcgctgt aacgccactg tgtttcttta ccattcacca
    34561 ccggccagcg cagaccgcgc gctttgtggt agtcatcgaa cggtgccaga tcgtgaccgt
    34621 gaccgcgacc aaaccatgcg tactcttcga acagcccttt ttgcagatag aagcccagct
    34681 cgcgggattc atcgttcagc tgatcttccg ccagttcgga taccgggaat ttgctcactt
    34741 cgggggtggc atacagaact tcgtacagcg ttttgccacg cagttccggt ttcttcgcca
    34801 gcagctcttc cggccatact tcttcagttt tgaagcggcg ggagaactgg actaactgcc
    34861 agagatccga tttcgcttcg cctggcgcct gtacctgctg acgccagaac tgagtacggc
    34921 gttcggcgtt accgtaagcg ccctctttct ctacccacat tgcggtcggc aggatcaagt
    34981 cggcggccag cgcactgact gtcggatacg gatcggagac gatgatgaag ttgcgcggat
    35041 cgcgccagcc cggcatacgc tcttcattga tgttcggccc ggcctgcatg ttgttggtac
    35101 acatggtcca gtaaacattc agcttgccgt ctttcagcgc acggtcttgt gccaccgcat
    35161 gaagaccgat tttcgccgga atggtgccgc tcgggatatt ccacttcttc tcacagatat
    35221 cacgatgttt ctcgttagtt accaccatgt ccgcaggcag acggtgagca aaggtgccca
    35281 cttcacgcgc agtaccacac gcagaaggtt gcccggtcag ggagaacgga ccgcaacccg
    35341 gctgggaaat tttgccggtc agcaggtgca ggttgtagac caggttgtta gcccacacac
    35401 cacgggtatg ctggttgaag cccatcgtcc agtaggagat gactttcttg ttcggatcgg
    35461 catacagctg cgccagttgt tctaactggt ctttcggcac gccggtcatt tcagcggttt
    35521 tttccagcgt atactcagca acgaaggctt tgtaatcttc aaagctcatc ggttcggagg
    35581 cgtcagaacc cggattcttc gctgcttttt ccagcggatg ggtcggacgt aaaccgtagc
    35641 cgatgtccgt cgcccctttg cgcaggttaa cgtgcttgct gaagaagtcc tgatttatcg
    35701 cattgttttg aatgatatag ttggcgatgt agttcaggat caccaggtca gattgcggcg
    35761 taaagatgat gccgttatcc gccagctcga agctacgatg ctggtaggta gaaagcaccg
    35821 ccacggtgac gttctggtta gagagacgac tgttagtgat gcgtgaccag aggatcgggt
    35881 gcatctccgc catgttagcg ccccacagca caaacgcgtc agcctgctcg atgtcgtcat
    35941 agcagcccat cggctcatcc ataccaaagg tacgcataaa gccaactact gccgacgcca
    36001 tacagtgacg tgcgttcggg tcgatgttgt tcgaacggaa gcccgctttg aacagcttgg
    36061 acgcggcata accttcccag atagtccact gaccagaacc gaacataccg atcgattccg
    36121 gccctttttc tttcagggcg gttttgaatt tctcttccat cacatcgaag gcctgatccc
    36181 aggtgattgg ggtaaattcg ccttctttgt catatttacc gtttttcata cgcagcagcg
    36241 gctgcgtcaa acggtcttta ccgtacatga tcttgggcag gaaatagccc ttaatgcagt
    36301 tcaggccacg gttaaccggt gcgtccgggt cgccctgaca ggccaccaca cgcccctgct
    36361 gcgttccgac cagaacgccg caaccagtac cgcagaaacg gcacggcgct ttatcccatt
    36421 taatggcttc ctgctgacca acaacggcgc gggcaacgcc cggcacgctg agaccggcag
    36481 ccgccgcagc ggccgcaacg gcgttagctt tcataaagct acgacgactg agtttcatgg
    36541 tgtttcctca ccttgctctt cctgctggtg ataaaccagc gacaccgcca gcacgccctc
    36601 tacgttgcgt actgactcaa tggtttggat cagcgtttcg ctgtcttctg cttccaccac
    36661 cacaatcaac tgaccgctcg gcgcgtcgct gacagcaact tcacagccgg gaaaggcgtt
    36721 cagttgggtg ctgatgtctg aaattcgttc gcttttggcc tgcacgacca ggctgcaaac
    36781 ttgccagtta gtgtgcatgg agatactccg cagttatggc tgataccgga cagctggctg
    36841 cacatgctcc acatccgttg caaagttggc tattaagttg cgcctggtag atcccggaga
    36901 gcgtcgggcg aaagataatt gccattggct cacagctatc ctgacagcgg cggcattcaa
    36961 ctgactgata cgccaggcac gcgtccccga tggtgaactg caaatcccag gccctggtgt
    37021 ggcgcggaga aaatagcgat tcggggcaag cctgcgcgca ggcgtaacag aagctgcact
    37081 cgttattttt gaaattaacg ctcggatagc cacccgtgcc gcgttgcaga atgttgtttt
    37141 cacaagcatt aatacaggcg tcgcagcggg tgcaatgggt cagaaaatga gattcatcac
    37201 cggaccaggg cggacggata ccgttactgg ctttgcgcca gcaaccagtg agtatgcccc
    37261 gacgggatgc atcaatcttc acattgacct tccatcatta acgcgctctg aaaattgaga
    37321 gcgaccaaat agaccgcata attaataagc catttttata gccgctaaga tattaaagga
    37381 tgtgtcaaag atgcataccc cgatcggggt aaaacctgta gcaggatcaa aagagtggcg
    37441 ggaagcgtgg caaaaacggg cttttgctca catttcaaat ggttataaac atatttatat
    37501 agcgattaat tcaccagaga tatttctgct ggtttgctcc ctcatcagaa tttaacacta
    37561 aaagagcagg taaaattgtc tgaatgttct ttaagttatt cataaagcaa attaataaat
    37621 ctgatgaata tgttaacctt cagcgacatc atcggtgaaa acctataaat gaagaaggaa
    37681 agcaaaaaaa tgaagaccat tctacctgaa gtattgtttg ccgctttcgc taccacttcc
    37741 gcctgggcgg cagaaagcgt ccagccactg gaaaaaatcg cgccttatcc acaagctgaa
    37801 acaggcatga agcgtcaggt gattcagtta accccgcaag aagatgaatc taccctgaaa
    37861 gtagaactgt taatcggtca gacgctggaa gtcgattgca atttgcatcg tctcggcggg
    37921 aagctggaaa gcaaaacgct ggaaggctgg ggctatgatt attatgtctt tgataaagtt
    37981 agctcgccgg tttccacgat gatggcctgc ccggatggca agaaagagaa gaaatttgtc
    38041 accgcgtatc tgggcgatgc tggaatgctg cgttacaaca gcaagctgcc gatcgtggtg
    38101 tatacgccag acaatgtaga tgtgaaatac cgcgtctgga aggcggaaga gaaaattgac
    38161 aacgcggaag ttcgctaaac tgttgtgaag tacggcaccc cgttggttgg ataaggcggt
    38221 tacgccgcat ccaacatcta acgcccgagc cggttgcctg atgcgacgct ggcgcgtctt
    38281 atcagtatac gccacatcat tacaacgcaa tatccgccac ttctttttga acgggttgcg
    38341 gtttcaactg cggtttcggc gtactatctg ctgcttgcgg tttgtcgtat ttcagcccca
    38401 gcacttcgct ggtgtactgc aactcgcgtt ctgttgccgc tacatcaccg ttcagcttgc
    38461 gtccataaga cggaacgatc gctttcaacg tagcctgcca ttgcgggctg gaaacgcgat
    38521 cgccaaatac tttttccagt agattcaaca taatcggcgc ggcggttgac gcccctggcg
    38581 atgcccccag cagcgccgca atggtccctt gctggtcact gaccacttcg gtacccagac
    38641 gcagtacgcc acctttctcg gcatcacgct tgataatctg cacacgctgc cccgcttgcc
    38701 acaaacgcca gtcctctttt ttcgcttgcg gatagtactc tttcaacgct tcaaaacgat
    38761 cctcttcact caacatcacc tgactcacca gatatttcac cagatcgaaa ttatccagcc
    38821 cgacgtgcat catcggcatc acgttagagg tggtggtgga actcatcaga tcccacaatg
    38881 aaccgttttt gaggaatttg gttgagaagg tggcaaatgg cccaaacagc actacgcgtt
    38941 taccgtccag aacacgggta tcgatatgcg gaaccgacat cggtggtgcg ccaacggatg
    39001 ctttaccgta aactttcgcc agatggcgat taaccacgtc tggattttcc gaaacaagga
    39061 actgtccgcc caccgggaaa ccggcgtagt ctttcgcttc cggaatcccc gattcctgta
    39121 acagcttcag cgccgcaccg cccgcgccga taaagacaaa tttggcgcgg atgttctgtg
    39181 cagtgccgtt tttcagatcg gcaacggtaa cggtccaggt gttatcgtca ttacgcttta
    39241 gggcgcggac ttcgctgctg agttgcagcg agaagttaga tttcttctgt aaggaagcaa
    39301 ttaactggcg ggtgatctca ccatagttca catcagtgcc aatttctgtt gcggatcgcg
    39361 cccttccatc actaacggtg cccactcttt gatctgcgcg tgatcttcag agtaacgcat
    39421 accgcgaaac agcgagcttt gttgcaacgc ggcgtaacgg gcgcgcagga aattaacgtt
    39481 atcctcgccc cagacaaagc tcatatgcgg aacggtattg ataaatgaac gcggagtacg
    39541 cagcacgccg cgctcgacct ggtgcgccca gaactggcgg gaaatctgaa atgcttcgtt
    39601 aatggcgact gctttttcaa tactgatgct gccatcggcg ttttgcgggg tgtagttcag
    39661 ttccatcagt gcagaatgcc cggttccggc gttgttctag ccgttcgaac tctcctgcgc
    39721 gcccccctcc aggcgctcca ccatggtcat cgaccattca ggctccagct cgcgtcaata
    39781 ggtccccaac gtggcgctca taatgccgcc gccaatcaac agtacatcag tttcctgctc
    39841 ctcggacgct ttcgcttttg ccgccatcga aacggcatta agccccacgg ccatcgagaa
    39901 gagcatggca gtcacttttt tcatcttgtt aatgccttac ttttagtcgc tttattgcag
    39961 gtgagatttg cgcggcatca acggtaacac ttaagtaaca acatttaaat aatgtttata
    40021 aattatattc taattttaga aacattataa tttcgttgat taattatagg gttattagaa
    40081 atgaagggat ttttagcata ttgacctgac ggcagcagtc cgccaggtca gcgtcgtaat
    40141 attatgccgt ccgggcaacg gcatcacgcg aagcggcatc gcgctcttcg cccgtcagct
    40201 cgctaagttg cccattgcgc atttccagca ggcggtcggc gtggataaag taatgatcat
    40261 catgactgat agcgaaaata gttttaccca tctcctgcat cagcggcagc aacacctgat
    40321 aaaactcacg acggaagtgt ggatcctgat ccaccgccca ttcatccagc aggataatat
    40381 cgcgttcttc tgccagcgcc agcaacagcg ccacgcgttt tttctgccct tttgataact
    40441 tcaggttaac aatacgcccg ttgcttaact caagcttatg agccattttc agctgcgcca
    40501 gccacttctc aaccagttgc gggttagcgg gttgcccctc cggccccagc agttgatcaa
    40561 acagccagac atcggtaaac actgccgaaa acagtttgcg ataatcttcc ggttgttcgc
    40621 cgctgacagg tttgccatcc agcaagattt cgccgctttg tggctgatac aagcccgtca
    40681 acaacatcgc cagcgtcgat tttccgctac cgttaccgcc aatcagaaac agcagctcgc
    40741 cacgtttgat ggtgagatta atcggaccaa cggaaaacgc gttatcctga taagcaaacg
    40801 tcacgttacg cagctccagc gtttgccagt tgggaaacgc ctgcgggcgc ggaaactcag
    40861 ctttgaaagg cgcgagcgcg aatttgttca gcttgttaaa cgccacctgc gccgtcagca
    40921 gcgtcggcaa tgcgccaacc gccgaaagca gcggcgtacg caggaataac agcgtcaacg
    40981 aataggtcgc agcaacgttg gtatcagccc aaccgagact gttcgccatc cagaacacca
    41041 ggccgatagc gcccagcatc atgatgtttg accagttcac ggcactaaga tggaaggtgt
    41101 ctgcgcgaat aatatgatgg cgatactctt gcgcatcggg gatgtagagg ttgttaaaca
    41161 catactcggc gcgttcccgg ttcagagtca gctctttgcg cccttcaagt acagtttgaa
    41221 aatccgtgta cagcttgtct tcggtttcac gtagggtcgc catatgtttg tacacccgcg
    41281 ccaccagcac aaaaccgccc cagatggtga tcgccatcca gatagccgtt accagcaaca
    41341 ttttgcccga cagcatccac agatacgccg atgaaccgat agtgagaatg atcccctgcc
    41401 ccagttccgg cagacgcaca aaagcaatgg tgatattgcg cacatcgctg gttaatcccg
    41461 ccagcaacga ggcgctaccg agttgttcaa tgcgctcgac gtgggtatcc agaatccgct
    41521 tgataaattc actacgcagt cggtaaacga agtgatgccc caaagtggtg agcgccagtt
    41581 gcgatccgag agtgactgcc atcaacagca gcaataatcc cagaaactcc ggtaacacca
    41641 gcagactggt atccgccgtt tcgataaggc gctgattgat aaaagcaatt aagccaatgc
    41701 ctaatgccgc actggcgagg cttagcgcca tcacactgat aaatggccag cgatactgcc
    41761 gccagacaag tacaagaagt tccatgcaga aaacccggac aatgaattac agcccgcagt
    41821 ttaaacatct tcgcgcgcac agcaataata attcttattt ttattctttt ttacctgcct
    41881 gacggaatgt caggttgtag cggcagtcgg tggtgagtgg atgaaacccc gctttcaacg
    41941 gttgaatacc gtgataaaac agccgcgatt caccgcccca taccaccaca tcgccatgtt
    42001 ccaacaacaa acgtttgagc ggatcattac gtttcaggcc accaaattga aaaatcgcgg
    42061 gtaagcccag agaaacagaa acaattggcg cgcgcagatc cggttcgtct ttatcctgat
    42121 gcagcgacag tttcgcgcca ggagcgtagc ggttgataag acaagcatct ggctggaaat
    42181 ctggatagcc cgccgccgta gccgcacgtt gacataaatc atgaaagctt tgtggcatag
    42241 cgggccacgg tttatttgtt tgcggatcaa tgggcgaata gagataacct tgccgatggg
    42301 tcgtccagcc cagatgccca cagttggtca tcgccaccga catggtatat ccgcctggag
    42361 cgaccatctg gcgaaacggc gactggctgg caacgtcatt aatatcgcgg attagttgct
    42421 ccgcagcatt aaaagcaaaa cgccgtaaaa ttaccgcacc agccgccagt ggctcttgcc
    42481 acggttcagc atcggcaaac agatccaaca ttacctctcc tcattttcag cttcgcggcg
    42541 cagcagttgc gctttacgcg acacgcccca gcggtaaccg gaaagtgtgc catcaccacg
    42601 gaccacccga tgacagggta ttacgatagc cagcttgttg gcggcacagg cgctggcaac
    42661 ggcccgtacc gctttcggtt tgccgatggc gttagccagt tgctgataac tgacggtttc
    42721 accacaaggt atcgtgcgca gtgcctgcca gacttgttgc tgaaaagcag tgccgcgaat
    42781 atccagcggt aacgtcagcg gcgtatcgcg ttgattgagg ctggcgatca cttcacgcac
    42841 atgttgctga aacgtcagat cggcaggcgc gttgtcggca gcgggaaaca tctgctgcaa
    42901 ctcgctgatt aatgtcgcgt catcatcgcc cagcaatatc gcgcaaatcc cccgctcgct
    42961 ttctgccacc aggcaacgac ccagctcaca atcagccagc gcgtaacgca ccgccagatt
    43021 ttcgccaccg tgacggaatt gtttagccgt catgcccagc gtttcatcgg ctttgcggta
    43081 gtaactgctg ctgtcgggga atccggcgtt aagaatagac gtcgtcacgc tctccccttt
    43141 cgccagcgat tcgcgcaaac ggcgagcgcg ccaggcctgt tgccaggctt taggcgtcat
    43201 tccggtagta gctttaaaca accgatgtag atgaaatgga ctcatcgcca cctggtcggc
    43261 taaggcttcc agcgttacag gcgtttcctg ttccagcagt cgacacgcgt gggtgatttt
    43321 atccaaccga tgttgccggg gattggcttt gtctggctga caacgtttgc aggggcgaaa
    43381 tccggcggcg agtgcctcgc tggcatttgc gtagaaggag acattttccc gcaaggcatg
    43441 tctggcgcgg caagaaggac ggcaaaagat acccgtggtg cgcacggcga aaacgaattc
    43501 gccgtcggca ttcgggtcgc gggctaagac agattgccag cgttgatcgt cagttaagca
    43561 tgtggcgttt ttcataatca gctccctggt taaggatagc ctttagtctg cccggtcacc
    43621 atcacgcaaa aaccaacaat cttgcgcttt aatttttttc gctgacaagg aagcttttaa
    43681 actgtggtga catccaggtt ttaaagctat cgccttcttt ggtgatcata taaaccgcca
    43741 gcccttcccg gcgaacaacc tctttggctt tctccggccc gagtaccatc aagccagtat
    43801 cccaggcatc ggcttccagc gccgtcggag caatcaccgt cacggatacc agattgtgtt
    43861 cgatgggacg cccggtttgc ggatcgataa catgggaaag acgtttgccg tccagttcgt
    43921 aatagttacg gtagctgcca gaggtgctaa tcccatgacc gttgatatcc accacagcct
    43981 gaaccgcgtt ttctttatcg gttggttttt gaatcgctac ccgccacggc tggccttcac
    44041 cgttcatacc acggctgttc agcgcgccgc ccaccgacac cagatagcgg gaaatccctt
    44101 cctgctccat caagcgtgcc aggtgatccg ccgcataacc ttcaccgacg gtggagagat
    44161 cgacatataa atccggcagg tctttttgca gatattgctg atgcgactga ttgatgaccg
    44221 tcaggtgctg taagccggtt ttggctttca tcgcatcgat ctgttcctgg ctcggaattt
    44281 gaaccggctg ttgttccggg ccaaagcccc acagattcac cagcggcccg acggtaatat
    44341 ccatcgcacc atcggtcctt gccccaatgc gcagcgaggt ggtgacgata tcggccatcg
    44401 cttcacttac cggccacggc gacaaacttt gcgagtcgtt aaagcgcatc agcgcggaat
    44461 cttttttata ggtcgaaagc agctgatcgt cggcgtccag ctgggtctga atcttttctt
    44521 taagttcggc actgcgtttg gcatcaatgc ccgggatgct ggcacgccag aaggtaccca
    44581 tggttttgcc ttcaagaacg gtaacttcag tagcgtgggt tttggcgggt tgtggttttt
    44641 gatcgcaacc aacaaagaag agcgcggcaa ccagcagtgc cacgcgggta aagcttattt
    44701 ccatacgtga ttatcctcat gcgaacggtc gcaagagtac accaaaaaac tgtgtttgta
    44761 cgctgaaaac aatgaaaaaa gggcccgcag gccctttgtt cgatatccat cgagattaga
    44821 actggtaaac cagacccaga gctacgatgt tatcagtgtt gatgccagcg tcacgagtga
    44881 actggttgtc gtccagcagg ttgattttgt agtcaacgta ggtggacatg tttttgttga
    44941 agtagtaggt cgcaccaaca tcaacatatt tcaggatatc ttcgtcgtcg tagttacgac
    45001 cattgatgac acccaggttt ttacctttag actgcaggta agccagggac ggacgcagac
    45061 cgaagtcgaa ctggtactga gcaacagctt cgaagttctg tgctttgttc gcccaaccca
    45121 agttgcctac gcgagttgcg ttgtaggtct gggtgtactg agcagctagg tagatgttgt
    45181 tcgcgtcgta tttcagacca ccggtgtagg tttcagcgcg gtcgccatta ccgatgtaac
    45241 gctcatcata acggttcaga ccgaagttct ggtcatcagt acgtttggag ctggaaactg
    45301 cagcaccgat accgaagcct tcgtaatcat aagtaataga accgccaacg ccgtcaccgt
    45361 tctgacgcag agcttcacga ccgttgttgg tcatgccttc accttccggg ctaccgtttt
    45421 tgccctgata ctgaacagcg aagttcagac cgtcaaccag accaaagaag tcggtgctac
    45481 ggtaggttgc gaagccgtta ccacgctgct gcatgaagtt gtcagaaccg taagtgtcac
    45541 caccgaattc tggcagtacg tcggtccagg aagttacgtc gtaaactacg ccgtagttac
    45601 gaccgtagtc gaaagaacct acatcctgga atttcagacc tgcgaatgcc acacgggtcc
    45661 aggagttgtt ttcgttttca gcgctgttgc cctggatctg atattcccac tggccgtaac
    45721 cggtcagctg gtcagtaacc tgagtttcac ctttgaagcc aagacgcatg taggtctggt
    45781 cgccatctac agacttgtcg tcagagaaat agtgcaggcc gtctacttta ccgtacagat
    45841 ctaatttgtt gccgtctttg ttgtaaactt cagcagcgtt tgctgcgcct gctaccagca
    45901 gagctgggac caggagggac agtactttaa ctttcatgtt attaaccctc tgttatatgc
    45961 ctttatttgc ttttttatgc cactgcatac tgattaaccc tcattaatca gtcggcaagt
    46021 ccattctccc caaaaatgca gaataatcca acacgaatat gatactaaaa cttttaagat
    46081 gtttcattta tcgctataga tgtttcaaaa tgtaaataca agggaacttt ttaagattat
    46141 tgcggaatgg cgaaataagc acctaacatc aagcaataat aattcaaggt taaaatcaat
    46201 aacttattct taagtatttg acagcactga atgtcaaaac aaaaccttca ctcgcaacta
    46261 gaataactcc cgctatcatc attaacttta tttattaccg tcattcattt ctgaatgtct
    46321 gtttacccct atttcaaccg gatgcctcgc attcggtttt tttacccttc tttacacact
    46381 tttcattatt ctgtgctacc acagaaaaac tataacgctt gttaactatt tcacaaataa
    46441 ttaacatcag cataatttcc agcaatcttt gtttatttgc aattaatttc gttgggcttt
    46501 ttgtaggtta tttgtacagc aaaatggcgc ttgtacatct atttcccccc atacaggatg
    46561 ataaatatca cgggagaata gagaatcatc aatcaggtaa gagtctggaa tttcacactg
    46621 taccctttat actgccctat cacttcgcga agttttaaca ggtcataaac acgaatgcgt
    46681 cagaaagaga caacggccac gacccgcttt tcactcctac cggggagcat tacccgcttc
    46741 tttttactgt tgatcattgt gttactggtg acgatggggg taatggtaca aagtgccgtt
    46801 aacgcttggc tgaaagataa aagttaccag attgtcgaca ttacccacgc aatccaaaag
    46861 cgcgtcgata cctggcgtta cgtgaccagg cagatctacg acaacattgc cgcgacgacc
    46921 tccccctcct ccggcgaagg tttacaagag acgcgcctga aacaggatgt ctactatctg
    46981 gagaaaccac gccgcaaaac ggaagcgtta atctttggct ctcacgacaa ctcaacgctt
    47041 gagatgactc agcggatgtc cacttatctg gatacattgt ggggtgcaga aaatgtaccg
    47101 tggtcgatgt attacctgaa tggtcaggat aacagtctgg tgctgatctc aaccctaccc
    47161 cttaaagatc tcacctccgg atttaaagaa tcgaccgtca gtgacattgt tgattcacgt
    47221 cgtgcagaaa tgttacaaca ggccaacgcc ctcgatgaac gcgaaagttt ttctaacatg
    47281 cgccgcctag cctggcagaa cggtcattac tttaccttgc gtaccacgtt caaccagccg
    47341 ggacatctgg caacggtcgt ggcttttgat ctgccgatta atgatttgat cccaccgggt
    47401 atgccgctgg acagtttccg ccttgagcca gacgcgacgg caacgggaaa caatgataat
    47461 gagaaagaag ggacggatag cgtcagtatc cactttaaca gtacgaagat tgaaatctcc
    47521 tcggcactca actctaccga tatgcgcctg gtctggcagg ttccttatgg caccttattg
    47581 ctggatacgt tgcaaaacat tctgctgcca ctgctgctga acatcggttt gctggcgctg
    47641 gcgttatttg gctataccac attccgccat ttctccagcc gcagtacaga aagcgtcccc
    47701 agcacggcgg tcaataacga attgcgcatt ttacgggcaa tcaatgaaga gatagtctca
    47761 ctgttgccgc tcggcctgct ggttcacgat caggaatcga accgcactgt cataagtaac
    47821 aaaattgccg atcatttgct gccgcatctg aatctgcaaa acatcaccac catggcggaa
    47881 cagcatcagg ggattattca ggcgacgatc aataacgagc tgtatgagat ccgcatgttc
    47941 cgcagccagg tcgcgccgcg cacacaaatt ttcattattc gcgatcagga tcgcgaagtg
    48001 ctggtaaaca agaaactcaa gcaggcgcag cgtctgtatg agaaaaacca gcaggggcgg
    48061 atgaccttta tgaaaaacat tggcgatgcg ctgaaagaac ccgcacagtc cctggcggag
    48121 agtgcggcta aactcaacgc cccggaaagc aaacaactgg cgaatcaggc agatgtgctg
    48181 gtgcggctgg tcgaagaaat acagttagcg aacatgcttg cggatgatag ctggaaaagt
    48241 gagacggtgc tgttctccgt gcaggattta attgatgaag ttgtgccttc agtgttgcct
    48301 gccatcaagc gtaaaggtct gcaactgctg attaacaatc atctgaaagc acacgatatg
    48361 cgccgcggcg atcgcgatgc cttacgacgt attttgctgc tactgatgca atatgccgtg
    48421 acctcaacgc aattgggaaa aatcaccctt gaggttgatc aggatgagtc ctctgaagac
    48481 cgcctgacat tccgcattct ggacacggga gaaggcgtaa gtattcatga aatggataat
    48541 ttgcacttcc cgtttatcaa ccagacccaa aacgatcgtt atggaaaggc ggacccgctg
    48601 gcattctggc tgagcgatca actggcacgt aaactgggcg gtcatttaaa catcaaaacg
    48661 cgggatgggc ttggtacacg ctactctgtg catatcaaaa tgctcgccgc tgacccggaa
    48721 gttgaagagg aagaagagcg tttactggat gatgtctgcg taatggtgga tgttacttcg
    48781 gcagaaattc ggaatattgt cactcgcctg ttagaaaatt ggggtgcaac ctgtatcaca
    48841 cccgatgaaa gattaattag tcaagattat gatatctttt taacggataa tccgtctaat
    48901 cttactgcct ctggcttgct tttaagcgat gatgagtctg gcgtacgaga aattgggcct
    48961 ggtcaattgt gcgtcaactt caatatgagc aacgctatgc aggaagcggt cttacaatta
    49021 attgaagtgc aactggcaca ggaagaggtg acagaatcgc ctctgggcgg agatgaaaat
    49081 gcgcaactcc atgccagcgg ctattatgcg ctctttgtag acacagtacc ggatgatgtt
    49141 aagaggctgt atactgaagc agcaaccagt gactttgctg cgttagccca aacggctcat
    49201 cgtcttaaag gcgtatttgc catgctaaat ctggtacccg gcaagcagtt atgtgaaacg
    49261 ctggaacatc tgattcgtga gaaggatgtt ccaggaatag aaaaatacat cagcgacatt
    49321 gacagttatg tcaagagctt gctgtagcaa ggtagcctat tacatgaaca atatgaacgt
    49381 aattattgcc gatgaccatc cgatagtctt gttcggtatt cgcaaatcac ttgagcaaat
    49441 tgagtgggtg aatgttgtcg gcgaatttga agactctaca gcactgatca acaacctgcc
    49501 gaaactggat gcgcatgtgt tgattaccga tctctccatg cctggcgata agtacggcga
    49561 tggcattacc ttaatcaagt acatcaagcg ccatttccca agcctgtcga tcattgttct
    49621 gactatgaac aacaacccgg cgattcttag tgcggtattg gatctggata tcgaagggat
    49681 cgtgctgaaa caaggtgcac cgaccgatct gccgaaagct ctcgccgcgc tgcagaaagg
    49741 gaagaaattt accccggaaa gcgtttctcg cctgttggaa aaaatcagtg ctggtggtta
    49801 cggtgacaag cgtctctcgc caaaagagag tgaagttctg cgcctgtttg cggaaggctt
    49861 cctagtgacc gagatcgcta aaaagctgaa ccgcagtatt aaaaccatca gtagccagaa
    49921 gaaatctgcg atgatgaagc tgggcgtcga gaacgatatc gccctgctga attatctctc
    49981 ttctgtgacc ttaagtccgg cagataaaga ctaatcatct gtaggccaga taagacgcgt
    50041 tagtgtctta tctggcattt gcaccgattg ccggatgcgg cgtaaacgcc ttatccggcc
    50101 tacgattccc attatttcaa cagattacat taaagtaggc cagataagac gcgtcagtgt
    50161 cttatctggc atttgcactg aatgccggat gcggcgtaaa cgccttatcc gtcctacgaa
    50221 tcccgcgatt tcctgaccct ctcggcatat accgtcagcg tctgttttat cacatccagc
    50281 gttaccggct tcgagaggca gctgtccata ccggactcca gacaccgctg cttctcttca
    50341 gccaacgcat tagcagttac tccgattacc ggcaacgtca gtcccaactg acgaatgcgt
    50401 tgcgtcaagc ggtaaccatc catatttggc atgttgacgt cgctaagcac gatatcaata
    50461 tgattcttac taagtacatt aagcgcatcg acgccatcat tcgcggtttt acattgatag
    50521 cccaacgatc ccaactgatc tgccagcaga cgtcggttaa tcggatgatc atccacgacc
    50581 agaatcatca tatcgtcgtt atcgctgacc gctttgtccg tcgacggcag agcgttagca
    50641 ggatcgtcgc tctccatctc gatcaaataa atacgcgcca acaatgccgg tagctcatgc
    50701 ggagcagcca cactgtgtac ccactcccct ggcgctttct ccagcggaat accaatatgg
    50761 cgacgacaga aggtcactac cgctctgccc tgccattttt tactcactac ctcgtcagtg
    50821 attaacacat cttcgggagt cggttcctgc ccttcgtatg ttgtaacgac gatgccgctg
    50881 cgctgcaaac tggtttccag gaactgacag agcgacgcat tgcggaccgc cagccagcag
    50941 cgtttaccac tcaacccttc cacgcctttt ttctgcgggt actgagcgcc gtacaacgga
    51001 atacgcacgg taaactggct gcccattccc ggttctgaat ctaccgagat atcgccgtcc
    51061 atcatgctga tcagtttttc acaaatcgcc agacccagac cggtcccctg gaagtttcgc
    51121 tgtacgcccg ttccaacctg gaagaaggga tcaaacaagc gcaccacttc tttcgccgga
    51181 atccccacgc cggtatcgcg aacacggata gagagataat cgccatcagc gcgaacatgc
    51241 aaaactatac agccggtatc ggtgaatttt atggcgttac tcaacaggtt ggagatgacc
    51301 tgctgtaaac gcatcgggtc gccatttaag gccactggca catccggttc aataaagcag
    51361 tacaagccta actgcttgcg taccaccagc ggtaaatagt tggcggtgat gtggttcatc
    51421 acttcacgcg gtgaaaactc acgcggttcg atcttcaact gttccgattc aatcttcgag
    51481 aaatcgagaa tatcgctgat aattttcaac aacaggctgg aagagttgtt cattgccgtc
    51541 accagacgat cgacgccttt cggtaactct ttggtttgca acagatccag gttaccgata
    51601 atgccataca gcggcgttcg cagctcatga ctgacggtgg caaggaacat cgattttgac
    51661 tggctcgcct gttccgctgc ttgtgccatc tcctgcaacg actcttccat cttcacgcgc
    51721 gaagaaacat ccaccagcac acaaatggcc acgttttcat tacgatagcg cgaatggacg
    51781 aagctgattt gcagattggt attgttgctg gtcaggacat caacaaaatt gacctgctgc
    51841 ccacagataa tttgcgtcag tcgttggcgg tcctcatgcg taagcatatt gagataggta
    51901 tgcgccagtt cgttacttaa aatattgacg ccatcagcgg tacgcaaaat gcagataccc
    51961 actggcgcgg aggcgacaat cttgcgattg aactgctcat gttcttccag tcgcagggcg
    52021 tcgctttccg ccggaatgaa aatacgtcgc tcgtacatcc gcgcgagagt aaacaatgca
    52081 gctccggcaa gcacattcag caaaattgcg ttaaggatca acatgcgaat gcgttccagc
    52141 accttatcaa ccggcaccga atacacgatg cttagcgatg agggtggcag atttttcttc
    52201 agcaccagct cccggaaccc ttccgtatag ccaaaccagg agcgttcctg catccagcga
    52261 ggatcgccct taattttact ttctggtccg gtaagcgaaa tcagggtatg accattttca
    52321 tcaagaatgg taacccccat cggcaacgta cccggtaaga aaaagttctc catccggatg
    52381 gtctgctcga cacccaaaag cgcctgcaac cggttcgcca gataaactgg cgtcaacgcg
    52441 taaaaatacc cgacgcaagg gcgcggacct tcgctgatcc agtagaggtt actgccgcta
    52501 tcatcttgtg gtgcatttcg atatttattg atgcgttcat gcaacgcttt caacgcggta
    52561 tcgcgttcca ctggcatatc acgcagaccg aaattggcca tgcagaggtt atcactgccg
    52621 attaaaaata cccggttgag atcgtaagcc gcagaaaaat tatcgcgcca gtagcgcata
    52681 aaccacgcca atgactccag agaacctcgc caggtgttac tcattgcgga acaatcggag
    52741 tcggcaaaca gcggttcaaa cgcaggcaca tccgcctgcg tttctcgtcc acgcggggaa
    52801 agcacaccgt tttctgccga taagcgattt tcggcgatgt acttaagctc tttcatcaca
    52861 tcagaggtgc gttgaataaa gcgctgagcc tgatcggaac tcagattaaa ttcctgacga
    52921 atttccgatt ctcgctgatg taacgcatta acgatgtaaa aaacggatga aaaagcaatc
    52981 aacagccaga gcactaacgc caatgctctg aacatgtagc gcgaggcttt cagggttgta
    53041 cgaaaagaag caaggtattt caaaggggag aagctccgcc tcaggtgacc gatggagtgt
    53101 ggttaaggta gcggtaaaag cgtgttaccg caatgttctg tcaccattgc aataatgcgt
    53161 atcgcttaga aaccattgcc ttaaccagga aagccggagt agatggacag ttctcagtac
    53221 gaaaaataag aaatgaccaa tctgccgacg cttgttaatg gttatatcct ggcgtcggca
    53281 gatcgcccag cagtcttatc tgaaaaaaga gagagccagg ttgtgtgaag ggatggatca
    53341 ccagtcaaaa cttttcacca gcgtcagctc gccagcatta cgcatcggta caataaatgt
    53401 ttcctgtttc tcattgaccg atccttcatc ggtgatcagc gtcagttggg cggtcgtcaa
    53461 ttccgtttcg ctgcgcccac catagtagtt gatatacacc tgatagcgcc cgtgaactgg
    53521 tgctggcatg gcgaaaatct cgggtccgta ccccgtcgtg acatccatat ccagtgcacc
    53581 actgtttttc agcacggtgt taccgtacca ggcgtgttcg ccatcgggcg tgacaacatg
    53641 gagatcgaga tcggtattgt ccgtatccca cgagagaacc agccgtaaac gtgcacgaat
    53701 agttcctgta cccggcgttg agtaaaactg cattttttgc cggctttgcc catccgggct
    53761 gatgacctgc acgctgttgc tgccttcagt gaaaatataa gggcgtgcaa atgaaccatc
    53821 ggattcaatt ctctgcggca tactggcacc gttgaccacc agccggccct gctgaacttt
    53881 acccgccgca gcataattct ttattttgcc gcggatctgc gctgagatat tttgatcatc
    53941 tgccatgttg acggacgacg ccgggtaatt gatcgattgt gaaaaactgg catcttcgcc
    54001 ctctgccgga tgccagccag agagcggtgc gtcaatctcg acttcctgca caccttcact
    54061 gtgagcgaca ggcgaaagtg ccaccagtaa caacggaaga aaaatctttc gcatagcatt
    54121 agtccattaa taattgtcgg gtcagcgttt cgatatagtt ctcatcaagc ccggtgggat
    54181 aaccatcaaa ggccaagtgc agatactcat gggttaaatc cagccgatcc tgaagcgtaa
    54241 agaagttacg aataaacagc cgtttctgct gccgatcggt ataggggaaa cctgagacta
    54301 aacgacagac cgcaaacacg tctggttcgt tgtaccccgt ctcgccttgt aatatacgcc
    54361 gccactgcgg catttttttc gccagccagg ctttcgcttt gggtaataac tggcaggttg
    54421 agcgtggagc tccccagcgg ctaaggctgt tgtcgggata agcaaacgcg agtatctggt
    54481 cgtaacgctc cccctgcccc gcctgggccg tggcttgccg ccaggaaagt gtcccttcgg
    54541 tagcgcgact gccatgataa tgaaccggat cgcctgcata gatgagctcc tgcgtccagg
    54601 cggtcatcgt tcgcgccccc gtggtcgctg gcgaagcaga aacacgctgc gtggcgctgc
    54661 tgtcggggat ggtcaggcaa tcaccttccc gattggcgtt ttgctgtaaa aaagtacgta
    54721 tcgccacagt catggctttc gctgcctcag gtggcgtact tttcgcctcg cgatccagaa
    54781 ctctggcaac gtactcttca cgatcgagat gcgactgcaa tttcagtttg cccttttcgc
    54841 tcagtaaggt cgtctcaccg tggctgacaa acgtaatgtg gttaccatta gtaaacgtca
    54901 cacgatagcg cccattaagt acgctgggtt ttactgccgt cgtactcttt tccgctgtga
    54961 ttttcttcag cggatagcgg gcaaacagct ccacctctac acattgccca ctggcaacct
    55021 gcgtcggcac tggcaaaacc cgattaagca ccgtcgcata acgggttagt accgttttgc
    55081 tggtgccact gccagtaacc cacagcggtg taccatcggt taaccagcca gcaaaaccac
    55141 cctgacggat ttcctgttta tcatcggcaa accagctcca ggttttcacc cgtacccgac
    55201 taccgagcat ggatgcgaca ccgattttcg cctcgtccag caccacatcc agtaagactt
    55261 cctgagcttt gttttgcgca ggtaacgtcg ctaatgaatc aagcagcgat ttaaccgtta
    55321 ccgaggtttc tggcttcagc gttgtaagag acgctaacca cgcaggtgcc tgacgctgtt
    55381 gccagtattg cccccacaca tctgcgccaa tatgcaaccg ctgcggcgca aaatagagcc
    55441 cacaggaacg taccagcgcg gtatcgcgcg ttatgctctc cccagcctga caacaataaa
    55501 cctcttccgg ggagtttccg cgacactggt agggctgctc cggttgatgg gtatcctcca
    55561 gccaggcgta aacatacagt ttccacaaac tccctaacgg cgtggtgagc gagtcaggta
    55621 atgctgagac tttggtcacg cctgatgacg acagctgata cagttgatcg tgctgcgcac
    55681 cgcgcagcac cagttgtaac ggcgtctcct ccgcaagcgt tggcagagta accaacgcca
    55741 atagccagac gattctgcgc cagttcactt atttcacctg catcccggtc cattcactcc
    55801 ctgccgcgac actttgctgc gcaggtgcat aggaacgcac ataacgagca ggaggcagaa
    55861 cgaattgccc tttttgcgag aagcgcagca aatggcggaa agtgaccgtt cccgtcagtt
    55921 ctttcaccgg caccatatag gccagttcgc ccatttcatt acgcgctttt tccagcaatt
    55981 gcccctgctg tttcgcggcg ttgggtttat tgactgaaat gccccatgtt gtgcgctcaa
    56041 cgtcagctcc cggcgggagc ggtacttcca cctgaccata gcgcagaact gcatcctgct
    56101 cgctggtaag cgtaatttca tcgagataca gcgcatcgct gtcaatctca ttgctggtca
    56161 ccggctgcag agtaaagctc atctcttctt caccggggat aagccgatac aactggcgtt
    56221 caacggtcac cggaatgtta ctttgttgag ccgtttttgc cggttcacgc cagcggacct
    56281 gcacatttgc ggcgataatt cgtcaccaaa agagagaatg tccggcacac cctaaccaac
    56341 ccaacgccag tcttcgcccc ctccagttaa cttatgttta gcccatgcgc ccgcaggcgc
    56401 aggcaaaaca actggaggca ttgtcgccat atatttcgcc agccagttca tggccagcgc
    56461 gcgctcaata gtggattgct cagcggtcaa accattaaaa tagcggccgt ctgggtagcg
    56521 tcaccaccgc tattgagcag cacaaccgtg tgcgccagcg gctgattgct gtttgccgct
    56581 ttatcccacg catattgctg aacctgtggc atcaccgtgg acttcaactg cgcttttttc
    56641 agcaacgtca tggttaagac gtttgccacc gcatctgcca gtggagattc cggtgtatcg
    56701 aggatcaggc tgtcattgat atcgcggatg tcttcctcag agaaatcttc atctttagtt
    56761 ccgcgccggg cgatagcttc atccccccct ttcaacaacg ttttgcacgg cagattcatc
    56821 tcctgtgccc acgccagcac cagcgcccga tgcaataacg gcatattatc cgcctgctcg
    56881 gcataactgt cgagcatatg ctgccagtat tccggttgtt gcgttacgcc gagcgcctgg
    56941 ctggcctgcc agtcggcgta ccatgcccat gccgtaagga aggcgtcacc attgccatct
    57001 tcaccccacc aggtaaagcg cgctccgggc cccgccagtt gcatcagccg cagacggtta
    57061 tcctgaatca tctgacgaat gtcgttagcg gcggcacttt gatgatcggc aagcgaacgc
    57121 caggcgagac tgagcgggat cagacggcta ccggtgttga tcaccccacc ccacggttca
    57181 tcgactaacg catcaagatt gttgcggaaa atctcctgcg gcgtttcact actttgtagc
    57241 cggatattac tcgcctgctc gggcaacatc agcgcgttct cgccaccgcc gagcatgaca
    57301 tttttctgct gttcaacggg ccagctgttg tccacaaaag acagttttgt gctaatgctg
    57361 tcctgcactt gcccattttg ttgcagttct gcacttaaca agccagattg ctgaatattc
    57421 tgcgtcagcg aaatataatt cgcccctttg tgcagcgtca gcgtctggcg catctcagcg
    57481 cctgcaaatt tagtcaccag cgccaccggt tcgttatcct gctgactgaa gataaacagt
    57541 cctgccgccg gtttgtcgcc cacgcgatac accgttggca tactccactt catgtagaga
    57601 tttttttccg aacgcagata agcacgcccc tgcccgacca gcccgtcgcc gttcatccca
    57661 cgcgcggtga tacgccagcg ggttaacgaa tcaggcatca ggaacgtgaa atacgctttg
    57721 ccttgtttat cggttgtgag tgacggcatc catgccgcgg tatccacctc ttcacgccgt
    57781 ggacgttcaa gcatttttac tcgccgctcg ctgcggttag tcgcgccagg cgcaaccggc
    57841 tcgctggaga gcgcctggtc atagctgata aacgacagac tggagctggt acgcacattg
    57901 ttacgcccca gcggatagaa aaatttgccg atattcggcg cgatttctgg ttgcagcgcg
    57961 tagatcattt catcgaccac gcctaccgtt agctgcgcag aaacaggttt acctttcagc
    58021 gacgaggtta at
//
LOCUS       DAEBXN010000012        55420 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.012, whole genome shotgun sequence.
ACCESSION   DAEBXN010000012 DAEBXN010000000
VERSION     DAEBXN010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55420
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.012"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(113..1486)
                     /gene="argH"
                     /locus_tag="KJL99_000717"
     CDS             complement(113..1486)
                     /gene="argH"
                     /locus_tag="KJL99_000717"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HBD4435569.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIGEDVYPILSLQSCLEKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            complement(1547..2323)
                     /gene="argB"
                     /locus_tag="KJL99_000718"
     CDS             complement(1547..2323)
                     /gene="argB"
                     /locus_tag="KJL99_000718"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HBD4435570.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRYAEQLPALFNGMPMGTRILA"
     gene            complement(2331..3335)
                     /gene="argC"
                     /locus_tag="KJL99_000719"
     CDS             complement(2331..3335)
                     /gene="argC"
                     /locus_tag="KJL99_000719"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HBD4435571.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            3489..4640
                     /gene="argE"
                     /locus_tag="KJL99_000720"
     CDS             3489..4640
                     /gene="argE"
                     /locus_tag="KJL99_000720"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HBD4435572.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASYGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGINAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPQPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            4992..7643
                     /gene="ppc"
                     /locus_tag="KJL99_000721"
     CDS             4992..7643
                     /gene="ppc"
                     /locus_tag="KJL99_000721"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HBD4435573.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYERNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            7826..9559
                     /locus_tag="KJL99_000722"
     CDS             7826..9559
                     /locus_tag="KJL99_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HBD4435574.1"
                     /translation="MHSTEVQAKPLSSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWTASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            9708..10559
                     /locus_tag="KJL99_000723"
     CDS             9708..10559
                     /locus_tag="KJL99_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBD4435575.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTEHSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10546..10887)
                     /locus_tag="KJL99_000724"
     CDS             complement(10546..10887)
                     /locus_tag="KJL99_000724"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HBD4435576.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(10889..11767)
                     /locus_tag="KJL99_000725"
     CDS             complement(10889..11767)
                     /locus_tag="KJL99_000725"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HBD4435577.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(11733..14030)
                     /locus_tag="KJL99_000726"
     CDS             complement(11733..14030)
                     /locus_tag="KJL99_000726"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBD4435578.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRVFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(14081..14401)
                     /locus_tag="KJL99_000727"
     CDS             complement(14081..14401)
                     /locus_tag="KJL99_000727"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HBD4435579.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(14416..15495)
                     /locus_tag="KJL99_000728"
     CDS             complement(14416..15495)
                     /locus_tag="KJL99_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HBD4435580.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            15804..16907
                     /locus_tag="KJL99_000729"
     CDS             15804..16907
                     /locus_tag="KJL99_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HBD4435581.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEGHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSRYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRV"
     gene            16908..18305
                     /gene="ptsP"
                     /locus_tag="KJL99_000730"
     CDS             16908..18305
                     /gene="ptsP"
                     /locus_tag="KJL99_000730"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBD4435582.1"
                     /translation="MTQQARTADGIRIEIAANIAHSVEAQAAFGNGAEGVGLFRTEML
                     YMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGGDKPVDYLNIPAEANPFLGYR
                     AVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSMEEILWVKEKLAEAKQQLRNE
                     HIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSNDLTQYLLAVDRDNAKVTRHY
                     NSLNPAFLRALDYDVQAVHRQGKWIGLCGELGAKGSVLPLLVGLGLDELSMSAPSIPA
                     AKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRMTQQDAPLVTAECITLESDWR
                     SKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFSTGLGFSFAIPHSKSEHIEQST
                     ISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIFSRLARRIMHEEFRNALVNAA
                     SADAIASLLQHELEL"
     gene            18317..18979
                     /gene="fsa"
                     /locus_tag="KJL99_000731"
                     /pseudo
     CDS             18317..18979
                     /gene="fsa"
                     /locus_tag="KJL99_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            18990..20091
                     /gene="gldA"
                     /locus_tag="KJL99_000732"
                     /pseudo
     CDS             18990..20091
                     /gene="gldA"
                     /locus_tag="KJL99_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
     gene            20366..20973
                     /locus_tag="KJL99_000733"
                     /pseudo
     CDS             20366..20973
                     /locus_tag="KJL99_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
     gene            complement(21000..21905)
                     /gene="yijE"
                     /locus_tag="KJL99_000734"
     CDS             complement(21000..21905)
                     /gene="yijE"
                     /locus_tag="KJL99_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HBD4435583.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(21999..24179)
                     /gene="katG"
                     /locus_tag="KJL99_000735"
     CDS             complement(21999..24179)
                     /gene="katG"
                     /locus_tag="KJL99_000735"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HBD4435584.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPIQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(24508..25398)
                     /gene="metF"
                     /locus_tag="KJL99_000736"
     CDS             complement(24508..25398)
                     /gene="metF"
                     /locus_tag="KJL99_000736"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HBD4435585.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(25747..28179)
                     /gene="metL"
                     /locus_tag="KJL99_000737"
     CDS             complement(25747..28179)
                     /gene="metL"
                     /locus_tag="KJL99_000737"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HBD4435586.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEFSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPSKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDYPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(28182..29342)
                     /gene="metB"
                     /locus_tag="KJL99_000738"
     CDS             complement(28182..29342)
                     /gene="metB"
                     /locus_tag="KJL99_000738"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HBD4435587.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLCRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            29619..29936
                     /gene="metJ"
                     /locus_tag="KJL99_000739"
     CDS             29619..29936
                     /gene="metJ"
                     /locus_tag="KJL99_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HBD4435588.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            30120..30728
                     /locus_tag="KJL99_000740"
     CDS             30120..30728
                     /locus_tag="KJL99_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HBD4435589.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGNYVVRRVEGGLSVEQQQK
                     LTQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(30789..31001)
                     /gene="rpmE"
                     /locus_tag="KJL99_000741"
     CDS             complement(30789..31001)
                     /gene="rpmE"
                     /locus_tag="KJL99_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HBD4435590.1"
                     /translation="MKKDIHPKYEEITASCSCGNVIKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            31204..33402
                     /gene="priA"
                     /locus_tag="KJL99_000742"
     CDS             31204..33402
                     /gene="priA"
                     /locus_tag="KJL99_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HBD4435591.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLTELKAVVEVLDGEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            33558..34583
                     /gene="cytR"
                     /locus_tag="KJL99_000743"
     CDS             33558..34583
                     /gene="cytR"
                     /locus_tag="KJL99_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HBD4435592.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            34675..35634
                     /gene="ftsN"
                     /locus_tag="KJL99_000744"
     CDS             34675..35634
                     /gene="ftsN"
                     /locus_tag="KJL99_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HBD4435593.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            35727..36257
                     /gene="hslV"
                     /locus_tag="KJL99_000745"
     CDS             35727..36257
                     /gene="hslV"
                     /locus_tag="KJL99_000745"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HBD4435594.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            36267..37598
                     /gene="hslU"
                     /locus_tag="KJL99_000746"
     CDS             36267..37598
                     /gene="hslU"
                     /locus_tag="KJL99_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HBD4435595.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            37665..38591
                     /gene="menA"
                     /locus_tag="KJL99_000747"
     CDS             37665..38591
                     /gene="menA"
                     /locus_tag="KJL99_000747"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HBD4435596.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQKMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            38684..39169
                     /gene="rraA"
                     /locus_tag="KJL99_000748"
     CDS             38684..39169
                     /gene="rraA"
                     /locus_tag="KJL99_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HBD4435597.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(39254..39499)
                     /gene="zapB"
                     /locus_tag="KJL99_000749"
     CDS             complement(39254..39499)
                     /gene="zapB"
                     /locus_tag="KJL99_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HBD4435598.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            39925..40770
                     /gene="glpF"
                     /locus_tag="KJL99_000750"
                     /pseudo
     CDS             39925..40770
                     /gene="glpF"
                     /locus_tag="KJL99_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
     gene            40810..42300
                     /gene="glpK"
                     /locus_tag="KJL99_000751"
     CDS             40810..42300
                     /gene="glpK"
                     /locus_tag="KJL99_000751"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBD4435599.1"
                     /translation="MVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPGWVEHDP
                     MEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAIVWQCRR
                     TAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSHERARRGELLFGTV
                     DTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEVRRSSEV
                     YGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNTGEKAVK
                     SENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFATKVQNT
                     NGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQA
                     DSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGLAVGFWQ
                     NLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            42530..43540
                     /gene="glpX"
                     /locus_tag="KJL99_000752"
     CDS             42530..43540
                     /gene="glpX"
                     /locus_tag="KJL99_000752"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HBD4435600.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            43637..44383
                     /gene="fpr"
                     /locus_tag="KJL99_000753"
     CDS             43637..44383
                     /gene="fpr"
                     /locus_tag="KJL99_000753"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HBD4435601.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(44388..44816)
                     /gene="uspD"
                     /locus_tag="KJL99_000754"
     CDS             complement(44388..44816)
                     /gene="uspD"
                     /locus_tag="KJL99_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HBD4435602.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(44843..45142)
                     /locus_tag="KJL99_000755"
     CDS             complement(44843..45142)
                     /locus_tag="KJL99_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HBD4435603.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(45354..45794)
                     /locus_tag="KJL99_000756"
                     /pseudo
     CDS             complement(45354..45794)
                     /locus_tag="KJL99_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
     gene            45895..46494
                     /locus_tag="KJL99_000757"
     CDS             45895..46494
                     /locus_tag="KJL99_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HBD4435604.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKIQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            46602..47369
                     /gene="tpiA"
                     /locus_tag="KJL99_000758"
     CDS             46602..47369
                     /gene="tpiA"
                     /locus_tag="KJL99_000758"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HBD4435605.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(47424..48179)
                     /gene="cdh"
                     /locus_tag="KJL99_000759"
     CDS             complement(47424..48179)
                     /gene="cdh"
                     /locus_tag="KJL99_000759"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HBD4435606.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGHTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(48286..49275)
                     /gene="sbp"
                     /locus_tag="KJL99_000760"
     CDS             complement(48286..49275)
                     /gene="sbp"
                     /locus_tag="KJL99_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HBD4435607.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(49595..50557)
                     /gene="pfkA"
                     /locus_tag="KJL99_000761"
     CDS             complement(49595..50557)
                     /gene="pfkA"
                     /locus_tag="KJL99_000761"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBD4435608.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(50738..51640)
                     /gene="fieF"
                     /locus_tag="KJL99_000762"
     CDS             complement(50738..51640)
                     /gene="fieF"
                     /locus_tag="KJL99_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HBD4435609.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(51788..52288)
                     /gene="cpxP"
                     /locus_tag="KJL99_000763"
     CDS             complement(51788..52288)
                     /gene="cpxP"
                     /locus_tag="KJL99_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HBD4435610.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            52438..53136
                     /gene="cpxR"
                     /locus_tag="KJL99_000764"
     CDS             52438..53136
                     /gene="cpxR"
                     /locus_tag="KJL99_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HBD4435611.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            53133..54506
                     /gene="cpxA"
                     /locus_tag="KJL99_000765"
     CDS             53133..54506
                     /gene="cpxA"
                     /locus_tag="KJL99_000765"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HBD4435612.1"
                     /translation="MIGSLTARIFAIFWLTLVLVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(54612..55286)
                     /gene="yiiM"
                     /locus_tag="KJL99_000766"
     CDS             complement(54612..55286)
                     /gene="yiiM"
                     /locus_tag="KJL99_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HBD4435613.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
BASE COUNT        13494 a        15016 c        14165 g        12745 t
ORIGIN      
        1 gaatgctgac aaatttctgc ctgatgcggc gtaaacgcct tatccgccta cgaagtgtcg
       61 cactttgtag gcgtggtaaa ctccccattt tcaggggagc aatataaaca aattactcta
      121 accgagcctg cgcaaaagca atcgcctgcg ccacctgctg cggtgagacg ccgccttttg
      181 ccgcacgctt ttcgaggcac gattgtagcg acagaatcgg atagacatct tcgccaatca
      241 cctgactgaa tttctgcaac tcactgagtg gcagatcttc cagcggtttg ccctgacgaa
      301 tggcttccac caccgcttca cccacaatat ggtgcgcctc gcggaaaggt acgcctttcg
      361 ccaccaggta atccgccagt tcagtggcgt tcgcataccc ctgctgcgcc gcttcctgac
      421 aacgcggacg tttcacctga atgccgtcca gtaccagcgc cgccatatgc aggcagtcca
      481 gccaggtatc gagcgcgtcg aacagacctt ctttgtcttc ctgcatatct ttgttgtaag
      541 ccagcggcaa acctttcagc gtcatcatca tgccggttaa cgcaccctgc acccggccgc
      601 atttaccgcg aatcagctcc agcgcatccg ggtttttctt ctgcggcatt aatgatgaac
      661 cggaagtcac gcggtccgaa agctccacaa accccgcttc gccggtgtta aagaaaatca
      721 gatcttcagc aaaacgcgac agatgcacca tgccgatagc ggcagcagaa agcagttcca
      781 acacatggtc acggtcagaa acgctgtcca gactgttacg ggtcgccgaa gcaaagccca
      841 gccagcctgc taactgttca cggtcgattt cataggccgt tcccgccagc gcgccacagc
      901 ctaatgggct gacatccaga cgcttaagcg catcctgcaa acggctttca tcacgcgcca
      961 gcatctcaac ataggccagg caccagtgcg cgaacgtcac cggctgggcg cgttgcaggt
     1021 gagtgtaacc tggcattacc gcgtcctgat tgttttgtgc ggtttccacc agcgccgatt
     1081 gcagctgccg gttagccgtc agtaactcgc taacggtatc tttgcaccac agtttcaggt
     1141 cagtcgctac ctgatcatta cggctacgcc cggtatgcag ctttttgcct aactggccca
     1201 ctttgtcgat cagtttgcct tccacccagc tatggatatc ttcggcgtcg ctttcaagga
     1261 tttgttgtgg cctggcgcga acatcttcca gcaacacgtt cagcgcctct tccagttgcg
     1321 cctgctcttc tgcggttaac acgccgaccg ttaccaaggc tttggaccag gccacagagc
     1381 caacaatatc ctgctccgcc agacggtaat caaagcgcag tgagtcgttg aattgtttga
     1441 accgttgatc tgctgcctgg gtaaaacgcc cgccccaaag tgccataact ctgtttcctt
     1501 attttgaaat tcaatgccgg aaagctgcgc ctccggctaa caaaacttaa gctaaaatcc
     1561 gcgtacccat cggcataccg ttaaacagtg ccggaagctg ctccgcataa cgccaagaag
     1621 cgatatctac cggacggccc agcgtgcggg ccgcatccag cgccgcgttc actttcacta
     1681 tcatgccgtc agtaataatg ccctgctcaa tcagttgttc tgctttcgcg gcggtcattt
     1741 cggcaatgcg ttgccctttg ccgtcgagaa tgccgctgac gtcggagagc aaaatcagat
     1801 ccgcgcccag cgttgccgcc agcgccgttg ccgcctggtc ggcattgacg ttcatcagtt
     1861 gcccttcgtc tgttacgcca atggagctga ccaccggcag ataaccgttc tccagcaagg
     1921 agttgataag cttaggcgaa cctggctgcg ccagtccaac atgacctaac tcttcatcaa
     1981 gctgggtcac tttgacgctg tcgccgtcac cgagaaacaa acctacggcc gcaatctgat
     2041 gtttcttcgc ccatgccaac agggttttat ttgccgttcc cgccagtgct ccggtgataa
     2101 tgtctatctg atcagcaggc gtcacccgca ggccgttttt ctttttcacc ggcagattca
     2161 gccctttcat cagctcatcc accacgcaac cgccgccgtg cacaatcacc agcggacgct
     2221 gatgtgactc acgataattc accaatgcgc taaacagacg ttccagcgcc tcttcactat
     2281 ccagcagtac gccgcccagt ttgataatta atggattcat cattgcaccc ttaaataaga
     2341 gactgcgttt ccgcatagcc gaaacgaata ttggcgcact gtaccgcttg tgccgccgcg
     2401 cctttcagta agttgtcttc ggtcgccaca ataatcagat gctcgccctg aacggcaaac
     2461 ccgatatcgc aaaatggcag cccaacgaca tttttcagcg ccggaacgcc tttgtcatac
     2521 agccgcacca gcggtttatg ggcatacgcc tgctgtaacg cttgcgcgac ttgcgcctgg
     2581 gtcacacccg atttcaggcg gcaggtaatg gtttcgagaa tgccgcgcgg gaaattgccc
     2641 agatgtgggg tgaagataac gtcagcaccg aggtgtgtgg cgatctctgg ttgatggcga
     2701 tgagtaaaga cgccatacgg ttgcaggcta acttcacaaa aactatttga aatcgctgcc
     2761 ttacgtcctg caccgctcac gccgctggtg gcgttgatca ccggccactg attgaggtca
     2821 agaagatcgg catcaatcaa cggtttcagc gccagctgtg ccgccgtcgg ataacagcct
     2881 ggcaccgcaa tcaaattcgc ttcttttaat ttattaccgc accactccgc cagaccgtag
     2941 gctgcctgtt ccagcagttc cgggtattga tgggtaaagc cgtaatattt ttcatagaag
     3001 gtggcgtcgt taacacgaaa cgcgccggaa aggtcgaaca ccacgcagcc cgcttcaaga
     3061 aactgcggcg ctaaatcgtg gctaacttca tgggcggtgg cgagaaacac tacgtccacc
     3121 cctgggctaa actcgctgat atccgacatc ggctgcaacg gcagctcaac gatgcctttt
     3181 agctgcggat gcaaatcgga gattaacttt cccgcatcat tgctttgcgc tgaaacagtc
     3241 aaagcggtta tgttcatatg cggatggcga tttacatagg tcactagctc tgcgccagcg
     3301 tagccgctgg cacccacaat cagcgtattc aacatcgggg ctattcacct tcttatgtct
     3361 ggttgccagg ttaaacgtaa aacattcacc ttacggctgg tgggttttat tacgctcaac
     3421 gttagtgtat ttttattcat aaatactgca tgaatattga tactatcatg accagaggtg
     3481 tgtcaacaat gaaaaacaaa ttaccgccat ttatcgagat ttaccgcgct ctgattgcca
     3541 caccttcaat aagcgctacg gaagaggcac tcgatcaaag caatgcagat ttaatcactc
     3601 tgctggcgga ctggtttaaa gatttgggct tcaatgtgga agtgcagcct gttccaggaa
     3661 ctcgcaacaa atttaatatg ctggccagtt acggacaggg ggctggcggc ttgttgctgg
     3721 cggggcatac cgatacggtg ccatttgatg acggtcgctg gacgcgcgat ccgtttacac
     3781 tgacggagca tgacggcaag ctttacggct taggcaccgc cgacatgaaa ggcttttttg
     3841 cgtttatcct tgatgcgcta cgcgatgtcg acgtcacgaa actgaaaaaa ccgctctaca
     3901 ttctggcgac tgctgatgaa gaaaccagta tggccggagc gcgttatttt gccgaaacta
     3961 ccgccctgcg cccggattgc gccatcattg gcgaaccgac gtcactacaa ccggtacgcg
     4021 cacataaagg tcatatctct aacgccatcc gtattcaggg ccagtcgggg cactccagcg
     4081 atccagcacg cggaattaac gctatcgaac taatgcacga cgccatcggg catattttgc
     4141 aattgcgcga taacctgaaa gaacgttatc actacgaagc atttaccgtg ccatacccta
     4201 cgctcaacct cgggcatatt cacggtggcg acgcttctaa ccgtatttgc gcttgctgtg
     4261 agttgcatat ggatattcgt ccgcagcctg gcatgacact caatgaactt aatggtttgc
     4321 tcaacgatgc attggctccg gtgagcgaac gctggccggg tcgtctgacg gtcgacgagc
     4381 tgcatccgcc gatccctggc tatgaatgcc caccgaatca tcaactggtt gaagtggttg
     4441 agaaattgct cggagcaaaa accgaagtgg tgaattactg taccgaagcg ccgtttattc
     4501 aaacgttatg cccgacgctg gtgctcggtc cgggttccat caatcaagct catcagcctg
     4561 atgaatatct ggagacgcgg tttattaaac ccacccgtga gttgattatc caggtaattc
     4621 accatttttg ctggcattaa aacgtaggcc ggataaggcg ctcgcgccgc atccggcacc
     4681 gttgccaaac tccggtgccg caataatgtc ggatgcgatg cttgcgcatc ttatccgacc
     4741 tacacctttg gtgttatgta tggcgatttt ttaacatttc cataagttac gcttatttaa
     4801 agcgtcgtga atttaatggc gtaaattcct gctatttatt cgtttgctga agcgatttcg
     4861 cagcatttga cgtcaccgct tttacgtggc tttataaaag acgacgaaaa gcaaagcccg
     4921 agcatattcg cgccaatgcg acgtgaagga tacagggcta tcaaacgata agatggggtg
     4981 tctggggtaa tatgaacgaa caatattccg cattgcgtag taatgtcagt atgctcggca
     5041 aagtgctggg agaaaccatc aaggatgcgt tgggagaaca cattcttgaa cgcgtagaaa
     5101 ctatccgtaa gttgtcgaaa tcttcacgcg ctggcaatga tgctaaccgc caggagttgc
     5161 tcaccacctt acaaaatttg tcgaacgacg agctgctgcc cgttgcgcgt gcgtttagtc
     5221 agttcctgaa cctggccaac accgccgagc aataccacag catttcgccg aaaggcgaag
     5281 ctgccagcaa cccggaagtg atcgcccgca ccctgcgtaa actgaaaaac cagccggaac
     5341 tgagcgaaga caccatcaaa aaagcagtgg aatcgctgtc gctggaactg gtcctcacgg
     5401 ctcacccaac cgaaattacc cgtcgtacac tgatccacaa aatggtggaa gtgaacgcct
     5461 gtttaaaaca gctcgataac aaagatatcg ctgactacga acgcaaccag ctgatgcgtc
     5521 gcctgcgcca gttgatcgcc cagtcatggc ataccgatga aatccgtaag ctgcgtccaa
     5581 gcccggtaga tgaagccaaa tggggctttg ccgtagtaga aaacagcctg tggcaaggcg
     5641 taccgaatta cctgcgcgaa ctgaacgaac aactggaaga gaatctcggc tacaaactgc
     5701 ccgtcgaatt tgttccggta cgttttacct cgtggatggg cggtgaccgc gacggcaacc
     5761 cgaacgtcac tgccgatatc acccgccacg tcctgctgct cagccgctgg aaagccaccg
     5821 atctgttcct gaaagatatt caggttctgg tttctgaact gtcgatggtt gaagcgaccc
     5881 ctgaactgct ggcgctggtt ggcgaagaag gtgccgcaga accgtatcgc tatctgatga
     5941 aaaacctgcg ttctcgcctg atggcgacac aggcatggct ggaagcgcgc ctgaaaggcg
     6001 aagaactgcc aaaaccagaa gggctgctga cacaaaacga agaactgtgg gaaccgctct
     6061 acgcttgcta ccagtcactt caggcgtgtg gcatgggtat tatcgccaac ggcgatctgc
     6121 tcgacaccct gcgccgcgtg aaatgtttcg gcgtaccgct ggtccgtatt gatatccgtc
     6181 aggagagcac ccgccatacc gaagcgctgg gcgagctgac ccgctacctc ggtatcggcg
     6241 actacgaaag ctggtcagag gccgacaaac aggcgttcct gatccgcgaa ctgaactcca
     6301 aacgtccgct tctgccgcgc aactggcaac caagcgccga aacgcgcgaa gtgctcgata
     6361 cctgccaggt gattgccgaa gcgccgcaag gctccattgc cgcctacgtg atctcgatgg
     6421 cgaaaacgcc gtccgacgta ctggctgtcc acctgctgct gaaagaagcg ggtatcgggt
     6481 ttgcgatgcc ggttgctccg ctgtttgaaa ccctcgatga cctgaacaac gccaacgatg
     6541 tcatgaccca gctgctcaat atcgactggt atcgcggcct gattcagggc aaacagatgg
     6601 tgatgattgg ctattccgac tcagcaaaag atgcgggcgt gatggcagct tcctgggcgc
     6661 aatatcaggc acaggatgca ttaatcaaaa cctgcgaaaa agcgggtatt gagctgacgt
     6721 tgttccacgg tcgcggcggt tccattggtc gcggcggcgc acctgcccat gcggcgctgc
     6781 tgtcacaacc gccaggaagc ctgaaaggtg gcctgcgcgt gactgaacag ggcgagatga
     6841 tccgctttaa atatggtctg ccagaaatca ccgtcagcag cctgtcgctt tacaccgggg
     6901 cgattctgga agccaacctg ctgccaccac ctgagccgaa agagagctgg cgtcgcatta
     6961 tggatgaact gtcagtcatc tcctgcgatc tctaccgtgg ctacgtacgt gaaaacaaag
     7021 attttgtgcc ttacttccgc tccgctacgc cggaacaaga attgggcaaa ctgccgttgg
     7081 gttcacgtcc ggcgaaacgt cgcccaaccg gcggcgtcga gtcgctgcgc gccataccgt
     7141 ggatttttgc ctggacgcaa aaccgcctga tgctccccgc ctggctgggt gcaggtacgg
     7201 cgctgcaaaa agtggtcgaa gatggcaaac agagcgagct ggaagccatg tgccgcgatt
     7261 ggccattctt ctcgacgcgt ctcggcatgc tggagatggt cttcgccaaa gcagacctgt
     7321 ggttggcgga atactatgat caacgtctgg tagacaaagc actgtggccg ttaggtaaag
     7381 agttacgcaa cctgcaagaa gaagacatca aagtggtgct ggcgattgcc aacgattccc
     7441 atctgatggc cgatctgccg tggattgcag agtctattca gctacggaat atttacaccg
     7501 acccgctgaa cgtattgcag gccgagttgc tgcaccgctc ccgccaggca gaaaaagaag
     7561 gccaggaacc ggatcctcgc gtcgaacaag cgttaatggt cactattgcc gggattgcgg
     7621 caggtatgcg taataccggc taatcttcct cttctgcaaa ccctcgtgct tttgcgcgag
     7681 ggttttctga aatacttctg ttctaacacc ctcgttttca atatatttct gtctgcattt
     7741 tattcaaatt ctgaatatac cttcagatat ccttaaggaa ttgtcgttat attcggcgat
     7801 atttttttca agacaggttc ttactatgca ttccacagaa gtccaggcta aacccctttc
     7861 tagctggaaa gccctgggtt gggcactgct ctacttttgg tttttctcta ctctgctaca
     7921 ggccattatt tacatcagtg gttatagtgg caccaacggc attcgcgact cgctgttatt
     7981 cagctcgctg tggttgatcc cggtattcct ctttccgaag cggattaaaa ttattgccgc
     8041 agtgatcggc gtggtgctat ggacggcctc tctggcggcg ctgtgctact acgtcatcta
     8101 cgggcaggag ttctcgcaga gcgttctgtt tgtgatgttc gaaaccaaca ccaacgaagc
     8161 cagcgagtat ttaagccagt atttcagcct gaaaattgtg cttatcgcgc tggcctatac
     8221 ggcggtggca gttctgctgt ggacacgcct gcgcccggtc tatattccaa agccgtggcg
     8281 ttatgttgtc tcttttgccc tgctttatgg cttgattctg catccgatcg ccatgaatac
     8341 gtttatcaaa aacaagccgt ttgagaaaac gttggataac ctagcctcgc gtatggagcc
     8401 tgccgcaccg tggcaattcc tgaccggcta ttatcagtat cgtcagcaac taaactcgct
     8461 aacaaagtta ctgaatgaaa ataatgcctt gccgccactg gctaatttca aagatgaatc
     8521 gggtaacgaa ccgcgcactt tagtgctggt gattggcgag tcgacccagc gcggacgcat
     8581 gagtctgtac ggttatccgc gtgaaaccac gccggagctg gatgcgctgc ataaaaccga
     8641 tccgaatctg accgtgttta ataacgtagt tacgtctcgt ccgtacacca ttgaaatcct
     8701 gcaacaggcg ctgacctttg ccaatgaaaa gaacccggat ctgtatctga cgcagccgtc
     8761 gctgatgaac atgatgaaac aggcgggtta taaaaccttc tggatcacca accagcagac
     8821 gatgaccgcc cgcaatacca tgctgacggt attttcgcgc cagaccgaca agcagtacta
     8881 catgaaccag caacgtacgc agagtgcgcg tgaatacgac accaacgtgc tgaagccgtt
     8941 ccaggatgtg ctgaatgacc ctgcgccgaa gaaactgatc atcgttcatc tgctgggtac
     9001 gcatatcaaa tacaaatacc gctacccgga aaatcagggc aagtttgatg gcaataccga
     9061 tcatgttccg ccaggattaa gcgcagaaga gctggaatca tataacgatt atgacaacgc
     9121 taacctgtat aacgatcatg tggttgccag cctgattaaa gactttaaag cggcagaccc
     9181 gaacggattc ctggtttact tctctgacca cggtgaagag gtttacgaca cgccgccgca
     9241 caaaactcag gggcgtaacg aagacaaccc gacgcgccac atgtacacca ttccgttcct
     9301 gctgtggacg tcggaaaaat ggcaagcgac tcatccccgt gatttctcac aggatgtcga
     9361 tcgtaaatac agcctggcgg aactgatcca cacctggtca gatttggcgg gcttatctta
     9421 cgacggttac gatccaaccc gttcagtggt gaatccgcag ttcaaagaaa ctacccgctg
     9481 gattggtaac ccgtacaaga aaaacgcgct gatcgattac gacactctgc cgtatggcga
     9541 tcaggtgggt aatcagtaga tgacctttgt atcgcgtctg gcgctatttg ccggatgcga
     9601 tactgccgca atgtttacat cgaactcccc tcaggccaca cttttcaggt ggcctttttt
     9661 aataatgcca gttactgttt tcccgtctac atacctaagg gaataacatg tatcacgacg
     9721 tcagctatct gctctcccgc ctgatcaacg gcccgctgtc cctgcgccag atttactttg
     9781 ccagcagtaa cggcccggtg cccgatcttg cgtatcaggt cgattttcca cggctggaaa
     9841 ttgtgctgga aggtgagttt gttgataccg gcgctggagc aacgttagtt cccggcgatg
     9901 tgctgtacgt tgccgctggt ggctggaatt tcccacaatg gaaaacaccc gctaccacct
     9961 ttagcgtgct gtttggcaaa cagcaactcg gcttcagcgt cgtgcaatgg gatggcaaac
    10021 aatatcaaaa tctggcgaag caacacgtcg cccgacgcgg cccacgcata ggttcttttc
    10081 tgctacaaac gctcaatgaa atgcaaatgc agccgcagga gcagcaaacg gcaaggctta
    10141 tcgtagccag cctgcttagc cactgccgcg atttgctcgg cagccaaatc cagaccgcct
    10201 cacgcagcca ggcactattt gaagctattc gcgattatat cgacgaacgc tatgcctccg
    10261 cgcttacccg cgaatctgtc gcacaggcat tctatatttc accaaactat ctctcgcacc
    10321 tgtttcaaaa aacgggggcc attggtttta acgaatacct gaatcacacg cgactggagc
    10381 acgctaagac gttattgaaa ggctatgacc tgaaggtaaa agaagtggcg cacgcctgcg
    10441 gctttgtcga cagcaactac ttctgccgtc tgtttcgtaa aaacaccgaa cactcgccgt
    10501 cggaatatcg ccggcagtac cacagccaac tgacagaaaa accgactact ccagaataag
    10561 atgggtttgc tgcggcgcag aaagcacttt gcgcaccgcg ctcattaccc gctgcggctc
    10621 acgcaggaat gcgtagatgc tgcattgcac atagcggcaa tgttcaaatc gctcggcacc
    10681 ggcaagctcg atatccgtaa tcaacagagc aacatccgcc cgacggatat cctcgtccgc
    10741 taaacgattc tccgttccca gcgcgccctg agtttcaatg ctgacgcccc acttctctaa
    10801 ctggcacaac ttttccagcc gttccgccgc catataagta tgcgccacgc cgcttacgca
    10861 ggcggttact gccaccaggt atgccatttt aacctcccac ggtaacctga aatccggccc
    10921 gttctgccat ttcgcgcatc gttgtcacat cggctgacga cggcgcaggc acctctttca
    10981 tcgaccatgt tttccccagc aggcggtatt tcggttcgcc gtactgatga aacggtaaca
    11041 gatggatctg cctgattttc agcgggatca gtacatccag cgcctgctgc atattctccc
    11101 ggctgagcgt gaaaccaggg atcaacggta aacgcgggat cacgttgaca ccctcactca
    11161 ccagcaaacg cagattctcc agcacgcgtg gcaggttcat cttcaccaca tcccgcgcct
    11221 gagtcgcgtc cataattttt aaatcgaaca gcacttcatc gcacaatttc gccagcggta
    11281 acagcttgct tgctggcgcg tctccggcag tttcaatggc gcatgagaca ccccacagcc
    11341 gcagtcgctg taaaaaacgg gtagcaaact ccgcctgcat taacacttcg ccgccagaaa
    11401 gcgtgacgcc gccgccggac gtgcgaaaga aaatgtcatc tttcatcact tcccgttcca
    11461 gagcgtcaag gctgatatcg cgaccaatcc gttcaaacgc cccggagggg cattcatccg
    11521 catcacgcaa acatttcgca cagtgcagac atttcgcctc tctgcgtacc gtctggattt
    11581 tgccggagat cgactccgga ttagcacacc acgggcaaag atgcggacag cctttaaaaa
    11641 agaccaccgt acgaatgccc tcgccgtcat tcagtgaata acgctgaatg ttgaaaatgc
    11701 gcgccacatc atcgcggcgc gtttccacaa cgttacagct gatgcgctgt ccggcggatg
    11761 atgtcatcct ggatctcctt cgacagttcg acaaagaagg cgctgtatcc ggcaacgcgc
    11821 accaccagcc cggcataatc ttgtgggcgc tgttgcgctt cccgcaacgt gtcggcgttc
    11881 accacgttaa actgaatatg ctgcaactta agctgggtaa acacccgtaa gaagtcggcc
    11941 agtttgcgta atcccgcttc accttccagg gtcgccggag tgaatttcac gttcagcaac
    12001 gtaccgttag acagcagcgt attatcgagc ttactgactg acttcagtac cgccgttggc
    12061 ccttgtgcgt cctgacccag cataggtgac aagccgccgt ctgccagttg ttccccggca
    12121 aaacgaccgt ctggcgtcgc gccaaccacc gatcccaacg gaacgtgagc agaaacggta
    12181 tacgatcccg gcgtgaagta accgccgcgc gggttctggt atttttccac ttctttgcag
    12241 tagtggcgca acagttcggc gctaatgtta tccacctcgt cgatatcgtt accgtatttc
    12301 tcaaagcggt taattaagcg agcgcggact ttttcgcctt ctggcgttgc aaagttggct
    12361 tttaataccg acagcaattc gtcaaaactt aaacgctgtt gctcaaaaac catccctttc
    12421 aacgcatgga gagaatcgct caggttggcg ataccaattc cctgtacgcc ggagaagtta
    12481 taacgcgcgc cgccatcggt aatatcgcga cctttttcca gacaatcgct gataaaagac
    12541 gagagcagcg gtacaggtgc ccagtcgcga tggccgatat cacaaatatt gctgccctca
    12601 accatcaggg tgatgtagtg gctgatcttg gcgcggatct gctccagtaa accttcataa
    12661 gtcagcgcag cattgccttc attttcatgc aggcagattt ccatcacttt cagcagatta
    12721 aacatcgcga tgtcatgcaa gccgtaggtt ctgccgggaa tagataattc cacacagccc
    12781 actacggaat agtcgcgcgc atcttccagc gaaacgccac ggttgaggaa cgctggcacc
    12841 accacttcat cgttaaagat ttgcggaata ccggtgccga ggcgaatggt ttcggcggtt
    12901 ttcatcagga acggcgtgtc gatcaaggcg ttagtgcgca cgccgaggtt cggttgcggt
    12961 aattgcacgc tttgataggc gtcaaggcaa aggaacgaaa gcacgttcac cgcgctacgt
    13021 ccgttctcgg ttaacccgcc gagcagtgcg gtatagccgg tcgggaaacc ggcgaaataa
    13081 cgcgcgctac tggtggagcg caacagcacg atgtcgttgc atttcaccca taaagattcg
    13141 agcagttctt tcaggaacgc cggatcttcg ccctgggtta aagatgcctg atagaacggc
    13201 aacatatact ggtcgaagcg ccctaaagac agcgaactgg cgttggattc gtattgcaga
    13261 atgatgttca tgtaccagaa taactggcac gcctgccaga acgtctgcgg cttatgttgc
    13321 gcgttatggc gggagatctc cgcaatagtc agcagctctt cgcgacgctg ggcatctgtg
    13381 cagtttgccg ccatcgtttc cgccagttcg gcgtaacgca aaatgtgttt ctgcgaggct
    13441 tccagcagta acaacgcggc ctgataaaag tgattctccg gctgttgctg acaatgttgc
    13501 tgcatctgtg ccaccagctc acccagaccg tgattcaaca ggcgtgggta atcaataata
    13561 atgtgcccct ggcctttatc cgtctggttg atgctgaaaa tctgcgtgtt ggtcgcggct
    13621 tttacttcat ctgtcatctg cccgttgatg aaatctttca tcgaacgttt ttcccagtac
    13681 gggaacaact cttcacggta gatacgttta tcttcttcgc tgatagcaaa gcggtcctgc
    13741 ggacgcgtcg ggaattgatc cagctctttc agcagccagt aagggtccat ttccggcgac
    13801 ataatcccgg cgcgcggttt tacggtgcgg ttaccggcaa tcagttcttc atcacgaatc
    13861 gaaatttcaa catgttcaag gatatacgct gttgctttcg cccggcgcaa tatcaccggt
    13921 tcgccttcgg tttgccgatg gctggcggta taaagcagcg cccgctccag cgagatttca
    13981 cgggtattgg caaacagtgc agttttgagg cgagagatac gattcgtcat gaggaactcc
    14041 tgtgggaaag cgaacccgcc cttgcgagcg ggagaagaac ttaagcggtt tgcgccaggt
    14101 gagcttcaat tttgctcata atggcgtcag cacgttttac tgcatcgctg atgttgacgc
    14161 gcacgatggt cttgccagca aaacgctctt caaacttgat gccgatatct ttggtcagaa
    14221 tcaccatatc agcgctcgcc acgtcttccg cagtcagttc attttccaga ccaattgacc
    14281 cctgggtttc tactttcact tcccagcctt tcgctttcgc ggcactttcc agcgcctctg
    14341 ctgccatata ggtatgggca acacctgaag gacatgcggt tactgcaata attttcgtca
    14401 tggctcgttc ctcaattaat taatttcaaa atccaaatcc aggtcgtctt ctttttcatc
    14461 agtcgaagac ccatttttac gcgccagact tttcagcacg ttcacacaaa cagccgtcac
    14521 cactgctccg acagccactg ctgcgatata acccaactta ccttcaacca ccggcagcac
    14581 aatcagacca ccccagcctg catagcactg cgcaccgacc agcgccgcag ttactgcacc
    14641 acaaactgaa ccgaccataa tggaaggaat gacacgcagc ggatcggcag cggcgaaagg
    14701 aatcgcccct tcagtaacgc caacacagcc catcaccagt gccgctttgc cggtttcgcg
    14761 ctcttctgcg gagaaatttt tacgaccaat cagcgtcgcc agccccatcc ccagcggcgg
    14821 gatacaaata cccacggcag caatggccac cacggtataa acaccctgag caacgcaaat
    14881 cagcatgaag gcataggcca ctttgttaac cggaccgccc atatcgaacg ccagcatcag
    14941 ccccatgatc accgccagca taacaatgct gccctgctgc atcccctgaa gccactgagt
    15001 caggctgttg gtcaacgccc ctacaggctc gcccaagccc cacatcatga tgcctgcggt
    15061 aatcagtgtg ccgatgatag gaataatgaa gatgggcatc accgagcgca gaactttatg
    15121 caccggaatt ttcttcaggt aatgcaccac gatgccgccg ataatcccgg cgatcagcgc
    15181 accaaagaag cccgcaccaa agctgttacc aacccaggca ccgatagcgc acggagccag
    15241 cgcagaacgc tctgcaatgg agtaaccaat gtaagcggcg aggaaaggca ccatcagcgt
    15301 caaacctgca acgccgatat caaacagttt tttcaaattc ggatcggcta cggcatccgg
    15361 cactgcgcct ttgccataca acatgacgga aaccgccagc aaaataccgc ccgataccac
    15421 gaagggaatc atgtgtgaaa cgcccgtcat taaatgctga cgggtatttt tcaggatctg
    15481 caccaactca ttcataagct gctcccgggc ttgcaaaagc ccatgtccat atttttgatg
    15541 gaggaaacaa taggcaatcg caggcggagc aaacagtgga taaaaaggcc atttactgga
    15601 cttttgtaat atcagtacaa aaatgcgatc cgcctcataa cttgcgataa tccggaagca
    15661 tgccgcaaaa cggcgtcttg tgagacgcgt cgcaaattga cggttacatt acatttgtcc
    15721 agcttttggc agatttgtca catggcaggc cgttaccgtg cgctcttatc ctgtaaacag
    15781 aaaaccgtac cgggagaaag gcaatggccc tgattgtgga atttatttgt gagctaccta
    15841 acggcgtaca tgcgcgtccg gcaagccacg ttgaaacgct gtgtaatact ttttcatctc
    15901 aaattgagtg gcataacctg cgtactgacc gtaaaggcaa cgccaaaagc gctctggcgc
    15961 tgattggcac cgatacgctg gtgggtgata actgccagct actgatttcc ggggccgacg
    16021 aacaggaagg gcaccagcgt ttaagccaat ggctgcgcga tgaattcccc cactgcgatg
    16081 cgccgctggc ggaagttaaa tctgacgaac tggaaccact gccggtttca ctgaccaatc
    16141 tgaatccgca aattatccgc gcccgcaccg tgtgcagcgg tagtgcaggc ggcattctga
    16201 cgccgatctc ttctttagat ctcaatgcgc tgagtaatct tcccgcagcc aaagacgttg
    16261 acgccgagca atccgcactg gaaaacggcc tgacgctggt actgaaaaac attgagtttc
    16321 gtctgctgga tagcgacggt gctaccagcg cgattctgga agcgcaccga tccctggctg
    16381 gcgatacttc cctgcacgaa catttactgg caggcgtcag cgccggatta agctgcgccg
    16441 aagcgattgt cgccagtgcg aatcactttt gcgaagagtt tgcccgttcc agcagcagat
    16501 acctgcaaga acgtgccctg gacgtacgcg acgtctgctt ccagttactc cagcaaatct
    16561 acggtgagca acgcttcccg gcaccgggca aactgacgca gtccgccatt tgtatggctg
    16621 atgaactgac ccccagccag ttcctcgaac tggataaaaa tcacctcaaa ggattgttac
    16681 tgaaaagcgg cggcaccacc tcacatacgg tgatccttgc ccgttcgttc aacattccga
    16741 cgctggttgg tgtggatatt gatgccctca ctccgtggca gcatcaaacg atttatatcg
    16801 acggcaacgc cggggcgatt gtggttgagc caggagaagc cgtagctcgt tattatcagc
    16861 aagaagcccg cgtacaggac gccctgcgtg agcaacagcg tgtctagctg acccaacaag
    16921 cccgtaccgc tgacggtatc cgcattgaaa ttgccgctaa catcgctcac tccgtggaag
    16981 cgcaggccgc attcggcaat ggtgcggaag gcgttggttt gttccgcact gaaatgctct
    17041 atatggatcg caccagtgca ccgggcgaaa gcgagctgta caacattttt tgtcaggcgc
    17101 tggagtctgc caacggacgc agcattattg tgcgcactat ggacattggc ggcgacaaac
    17161 ccgttgatta tctaaacatt cccgcagagg caaacccgtt cctcggttat cgcgccgtgc
    17221 gtatttatga agaatacgcg tcgttattta ccacacaact acgctcgatc ctgcgcgcct
    17281 ccgctcacgg cagcctgaaa atcatgatcc cgatgatctc ctcaatggaa gagatcttat
    17341 gggtgaaaga aaaactggca gaagccaaac agcaactacg taacgaacac attccgtttg
    17401 atgagaagat ccagctcggc attatgctgg aagtgccgtc ggtgatgttc atcatcgatc
    17461 aatgctgcga agagattgat ttctttagta ttggtagtaa tgacctgacg caatatctgc
    17521 tggcagtgga tcgcgataac gctaaggtta ctcgtcacta caacagcctg aatccggcat
    17581 tcttgcgggc gctcgattac gacgtgcagg cggtgcatcg ccagggtaaa tggattggtc
    17641 tgtgcggtga gctgggagcg aaaggttccg tgctgccgtt gctggtcggc ttagggctgg
    17701 atgaactcag catgagcgca ccatcaattc cggcggcgaa agcgcggatg gcgcaacttg
    17761 atagccgtga gtgccgcaag ttgctcaacc aggcaatggc ctgccgtact tcgctggaag
    17821 tggaacacct gctggcgcaa ttccgcatga cccaacagga cgcaccgctg gtcaccgccg
    17881 agtgcatcac gctggaaagt gactggcgca gcaaagaaga agtgctcaaa ggcatgaccg
    17941 ataacctgct gctggcgggc cgctgccgct atccgcgtaa actggaagcc gacttatggg
    18001 cgcgcgaggc cgttttctct accggtctgg gctttagttt tgccattcca cacagcaaat
    18061 cagaacacat tgagcaatcc accatcagcg tggcgcgtct gcaagcgccg gtgcgctggg
    18121 gcgatgatga agcgcaattc atcattatgt taaccctgaa caaacacgct gcgggcgatc
    18181 agcacatgcg cattttctcg cgcctcgctc gccgcatcat gcacgaagaa ttccgtaacg
    18241 cgctggttaa cgccgcctct gccgacgcta tcgccagcct gctgcaacat gaactggaac
    18301 tgtaaaagga aacatcatgg aactgtatct ggacaccgct aacgtcgcag aagtcgaacg
    18361 tctggcacgc atattcccga ttgccggggt gacaactaac ccgagcatta tcgctgccag
    18421 caaggagtcc atctgggaag tgctgccgcg tctgcaaaaa gcgattggtg atgagggcat
    18481 tctgtttgct cagaccatga gccgcgacgc gcaggggatg gtggaagaag cgaagcgcct
    18541 gcgcgacgct attccgggta ttgtggtgaa aatcccggtg acttccgaag gtctggcagc
    18601 aattaaaata ctgaaaaaat agggtattac tacacttggc accgctgtat atagcgccgc
    18661 agaagggtta ttagccgcac tggcaggggc aaaatacgtt gctccgtatg ttaaccgcgt
    18721 agatgcccag ggcggggacg gcattcgtac ggttcaggag ctgcaaacgc tgttagaaat
    18781 gcacgcgcca gaaagcatgg tgctggcagc cagctttaaa acgccgcgtc aggcgctgga
    18841 ctgtttactg gcaggatgtg aatccatcac cctgccctta gatgtagcgc aacaaatgct
    18901 caacacccct gcggtagagt cagctataga gaagttcgaa cacgactgga atgccgcatt
    18961 tggcactact catctctaaa ggagcaatta tggaccgcat tattcaatca ccgggtaaat
    19021 acatccaggg cgctgatgtg attaatcgtc tgggcgaata cctgaagccg ctggcagaac
    19081 gctggttagt ggtgggtgac aaatttgttt tagtttttgc tcaatccact gtcgagaaaa
    19141 gctttaaaga tgctggactg gtagtagaaa ttacgccgtt tggcggtgaa tgttcgcaaa
    19201 atgagatcga ccgtctgcgt ggcatcgcgg agactgcgca gtgtggcgca attctcggta
    19261 tcggtggcgg aaaaaccctc gatactgcca aagcactggc acatttcatg ggtgttccgg
    19321 tagcgatcgc accgactatc gcctcgaccg atgcaccgtg cagcgcattg tctgttatct
    19381 acaccgatga gggtgagttt gaccgctatc tgctgttgcc aaataacccg aatatggtca
    19441 ttgtcgacac caaaatcgtc gcgggcgcac ctgcacgtct gttagcagcg ggtatcggcg
    19501 atgcgctggc aacctggttt gaagcgcgtg cttgctctcg tagcggcgcg accaccatgg
    19561 cgggcggcaa gtgcacccag gcggcgctgg cactggctga actgtgctac aacaccctgc
    19621 tggaagaagg cgaaaaagcg atgcttgccg ccgaacagca tgtagtaacc ccggcgctgg
    19681 agcgcgtgat tgaagcgaac acctatttga gcggcgttgg ttttgaaagc ggcggctggc
    19741 ggcggctcac gcggtgcata acggcctgac cgctatcccg gacgcgcatc actattatca
    19801 cggtgaaaag tggcatttgg tacgctgacg cagctggtac tggaaaacgc gccggtggag
    19861 gaaatcgaaa ccgtagctgc acttagccat gcggtaggtt tgccaataac tctcgctcaa
    19921 ctggatatta aagaagatgt cccggcgaaa atgcgcattg ttgcaaaagc atcttgtgca
    19981 gaaggtgaaa ccatccacaa catgcctggc ggcgcgacgc cagatcaggt ttacgccgca
    20041 ctgctggtag ctgaccagta cggtcagcgt ttcctgcaag agtgggaata acctactcca
    20101 aactcccggc ttgtccggga gtttgaacgc aaaattgcct gatgcgctac gcttatcagg
    20161 cctacgcaat ctctgcaata tattgaattt gcgtgctttt gtaggccgga taaggcgttc
    20221 actccgcatc cggcactctg tgccagtaat ctgaaactcc cacgttgttc aggagttttt
    20281 tatccgcttc tggacatttt ctccacagaa caatcgttag cccggaaggt cgaatctgtg
    20341 actcttattt tcacggacga accagatgaa agcttcactg gcgctgctca gcctgctaac
    20401 ggcatttacc agccactcct taaaatctcc cgccgttcca ccaaccgtgg tacaaatcca
    20461 ggccaatacc aaccttgcca tcgcagacgg tgccagacag caaattggca gcacgctatt
    20521 ttacgatcgc agcttaccta tcctggcggt gatgttccgc aagaacgcgg tgtatgttcc
    20581 gatgtggtga tccgcgcatt gcgcagccag aaggtcgatt tgcagaaact ggttcacgaa
    20641 gatatggcga agaattttgc tgagtacccg caaaaatgga agttaaagcg cccggacagc
    20701 aacatcgatc accgtcgggt gcctaatctg gaaacctggt ttagccgcca caacaaaaca
    20761 cgccccatca gtaaaaatgc cggtgactat caagcgggcg atattgtctc ctggcgatta
    20821 gataacgggc tggcgcatat tggggtggcg tcagatggct tcgcccgcga cggtacgccg
    20881 ctggtgatac ataacattgg cgcaggcgcg caggaagaag atgtgctgtt tagctggcgg
    20941 atggtggggc attatcgtta ttttgttaaa taagagatcg ccccacatga agaaaaaatt
    21001 cagatgcttt ttacactgac agcttctttt ttcttacggc tcaccagcgc cagtgccagc
    21061 acaatcagca caataccgct accttcaacg gcccccggat tctcaccgag cagccaccag
    21121 gaaaagagta cgccacaaac gggaacggcc agtgtgctta agctggcaat actggcaggc
    21181 aagtttttca gcacaaacag ccataagctc cacgctagcg ccgtcgccag aatcgcactg
    21241 taggccagcg cccagaacac ggtgggctgc cagtcaattt cacgttgcgg caccagtaaa
    21301 gcgaccacac tcatcaccag cgccgcatac agcatctgcc aggatgttaa cgataataaa
    21361 tccacgcgcg gatgacgggc gtataggcgt ttagcaacaa tcgcgctcgc cccccaacta
    21421 acaccggaga ggattgccag catggcactt ttcatcgaag agaaatcgag ttgccacggc
    21481 tgcaacacca aaaataagcc gaaagcggca atcagaatcg cgaaatattg cccacgtcgc
    21541 aggcgttcac cgagaaacaa cgcggcgaaa atcaccaccc agaacggcat ggtatagctc
    21601 aggatcgcca ccttccccgc acctccgctg accaacgccc actgcgccag accaaccatc
    21661 ccgcaggttt gtaacagggc aatggctaag gtgtatttaa acggcgtcgg gcgcattccg
    21721 cgaccacgta ataaaaggac gatgaataaa acgagagcgc cgaaaatgca gcgtaaggcg
    21781 gtaaagtcga aggcaccgat gtaactggtg acttgcttca tgaaaatcca gctataactc
    21841 cagataagtg tgagcacaac caagccactg attgccagtg ggttgctctt tcctgcggca
    21901 gacatgagag atccagtgtg tagttttcct gtggacacta tacgccggta aagaaacttc
    21961 agcgactgcc tgaaagacag tcgctgaaga aggtcagatt acagcaggtc gaaacggtcg
    22021 aggttcatca ctttcaccca tgccgctacg aagtctttaa caaacttctc gtgggcatcg
    22081 ctgctggcgt aaacttccgc tactgcacgc aggacagagt tagaaccaaa taccagatcc
    22141 gcacggctgg ctgtgtattt cacttcgccg gtttcgcggt cacgtccttc gaacagctct
    22201 ttcgattcgt cagtcgcttt ccactcgtaa cgcatatcca gcaagttcac gaagaagtca
    22261 ttgctcaata cgccaacgcg gtcagtgaag acgccgtttt tgctgccatc gaagttggca
    22321 cccagtacac gcatcccgcc caccagcgca gtcatttccg gcgcggtcag cgtcagttgc
    22381 tgtgctttgt cgatcagcag tgactcggtg gtggaaacgt ccagacgagc gcgatagtta
    22441 cggaaaccgt cagcaattgg ctccagcagc tcaaacatct caatgtcagt ctgatcctga
    22501 cgcgcatcaa cgcgacccgg cgcaaacggt acatgaatgc tcaaacctgc ggcgcttgcg
    22561 gctttctcaa caccaaccac accagccagc acgatgatat ccgccagcga ggctttacca
    22621 gactctttct ggattttctc cagaacaggc agagcacgaa cggctgcggc gttcacatcc
    22681 cagtcgcgct gcggcattaa tgccagacgc gcaccgttgg caccaccgcg tttgtcgcca
    22741 ccacggaagg tagaagcaga tgcccaggcc accgatacca gctcactaac agacagacca
    22801 gaatccgcaa tcgcgaattt cagatcgata atgtcctgct cggtcgggtt gtagatcggc
    22861 tgcggcagcg gatcttgcca gatcagatct tctttcggca cttccggccc gatgtagcga
    22921 gatttcggcc ccatatccct gtgcgtcagt ttgaaccagg cacgggcaaa ggcttcgttg
    22981 aacgcctgcg gatcgttgag gaaacgacga gagatcttct cgaactcagg atcaaaacgc
    23041 agcgtcaggt cggtcaccag cattgtcggt ttacgtttct tcgacggatc gaacggatcc
    23101 gggataattt ccggtgcgtc taccgcttcg aactggattg ctccagccgg actgcgggtc
    23161 tgtacccact catacttgaa cagattctcg aagaaatagt tgctccactg gatcggcgtc
    23221 tgggtccaga ctacttccag accagaggta atggcatctg cgccaacgcc gctgccgtaa
    23281 gtgctcgccc agcctaaacc ttgttcttca atcggtgcag cttctggatc aggacctacg
    23341 tttgatgtcg gaccggcacc gtgggtttta cccagcgtat gaccacccgc aatcagcgcc
    23401 acggtttctt cgtcgttcat gcccatgttg ccgaaggtcg cgcggatagc tgctgccgca
    23461 gaaagcggtt cgccgctgtg atccgggcct tccgggttaa cgtaaatcag gcccatctcg
    23521 gttgcaccca gcggtgcttt cgccagcgct tccggatgac ggtgagtcag ccaggctttt
    23581 tcatcacccc agttaacatc cagatccggt tcccagacgt cttcacgacc ggcaccaaaa
    23641 ccgaaggtac ggaagccgga gttttccagc gccacgttac ctgcgaggat aaacaggtcg
    23701 gcccaggaga ttttctgacc atatttctgt ttgattggcc acaacaggcg acgcgcttta
    23761 tcgaggctta cgttatccgg ccaggagttc agcggtgcaa aacgttgctg accacgaccc
    23821 gcgccgccgc gtccatcgat tgaacggtaa gtacctgcgc cgtgccaagc catacgaata
    23881 aacagaccgg cgtagctgcc ccagtcggct ggccaccacg gctgagattc tgtcagcagg
    23941 gctttcagat cttttttcag gccgtagtaa tctaatttgc tgaattcttt gcggtagtca
    24001 aagtcctcac ccagcgggtt agaacgatta gaatgttggt ttaacaggtc aacacggagt
    24061 tgatttggcc accagtcgcg agtggttgtg cccgcccccg cgctctggtc gtgaccgccc
    24121 tgatggaacg ggcatttgcc agtggctgtg gtgttatgga tatcgtctga cgtgctcatc
    24181 aatgtgctcc cctctacagt gttaccgtta cgatacacag cgttagagag aagttataat
    24241 tgaattaatc cacggatgcg atagttgaga tcttacatat tgttggttaa agagatgtag
    24301 atcaaattga tcgtaattaa gcttttttga ttatgtccga attcggacaa atgctttata
    24361 aaaaagggtt ttagcttcat atccttcaag taatgaggga gaaaacaagg ctgacaacct
    24421 tattttcccg ccctcatttc gaggcagcat tttgtgctct gtttaaaatt tgtgatcact
    24481 gtgtgatttt cacaaaagcc acactattta taaaccaggt cgaacaccca gcgtatggca
    24541 aatcgcgtaa ctcatttcag cgcggttaag cgtatagaag tggaaatctt tcactccttc
    24601 acggcttaaa atcttcacca tatccatggc aatattcgcg cccaccagtt tgcgggtttc
    24661 ggcatcgtca tccagcccgt caaacatttg cgccatccac gccggaatac gcacgttggt
    24721 catgtcggca aatttcttcg cctgtttaaa gttagatacc ggcaaaatgc ccgggatgat
    24781 ttccacatca atgcccgccg atacacagcg gtcacgaaaa cgcaggtagc tttcgacatc
    24841 gaagaagaac tgagtaatcg cgcggttggc tccggcatcc actttgcgtt tcagattaag
    24901 caaatccgcc tgagcgcttt ttgcttccgg gtgaacttcc ggatacgccg ccacggatat
    24961 atcgaaatct gccacttctt ttaacagcgt caccaggtca gaagcataca tttctggctt
    25021 accacttccc ggcggcagat cgccacgcag tgccacgata tgacgaatac cgttattcca
    25081 gtagtcgcgt gcaatggtgc gcagctcgtc gggcgtcgca tcaatgcaag taagatgcgg
    25141 tgccgcttcc agaccagtgc gatctttaat gcctttaata atgctgtgcg tacggtcgcg
    25201 ctcgccggag ttcgcgccat aggtcaccga tacaaacttc ggtttcaggc tgctaaggcg
    25261 atcgatggag ttccacaggg tctgctccat ttcactggta cgcggcggga aaaactcgaa
    25321 cgaaacgtta atctgcccct ggacttctgc caggctctga ttcagggcat cccgctggct
    25381 ggcgtgaaaa aagctcatac cttacctcat caatcgcttg tagttatatg ttgtgttgtg
    25441 cacatctatc catttagacg tccagatgta aagatgaagg aaaagccgaa gggcgtcaac
    25501 cgaaaaatga ccaatgatgg tgaggaaaaa tcagggaaga tgaaaaaact tcaggatggt
    25561 aagaaaaaga aaatccgata tgcaaagcaa accggactgc aaatataaac taaccatgta
    25621 cttattgctt cttgccggat tcgtcgtaaa cgcttcatcc ggcctaattc tatgataatt
    25681 caatatattt cattagaaac catagacccg aaaacatgag taccgggcat tattaaattt
    25741 ctgaaattac aacaactgtg ccagccggtt gatatccgac tgaatcgccc cggcggtgac
    25801 gtcgcgccca gcgccaggcc cacggatcac cagcggatta tcgcggtacc agcggctttc
    25861 gatggcaaag acgttatcgc atggcagcag tgatgccagc ggatagtctt cacgcaccgc
    25921 ctccacgccc acacgcgctt tgccgttggc atcgaaacgc gccacgtagc gcagcaccag
    25981 ccccatttcg cgggccgctt ccagccgttg caccatctgc tcgttaagtt catcgccatt
    26041 ttcaaagaaa tggtcgatgc tgccgccttc gcaatgagca ggcaccagcg attccacacg
    26101 tacctggtcc ggttcaatgt tgtaacccgc ttcacgcgcc agaatcacca gcttgcgcat
    26161 tacgtctttg ccggagaggt cgtcacgcgg atcaggctcg gttaagccct gctgccacgc
    26221 ctgatccacc agctcggtaa acggcacgct accgtcgaat tgcaggaaca gccaggagag
    26281 cgtgccggag aagatcccac tgatcgacaa aatggtatcg ccgctgtcga tcagatcgcg
    26341 cacggtatgg ttgatcggca agcccgcacc aacggtggcg ttgtatagcc agtgacgccc
    26401 ggttttttcg aaggcgtcgt ggatctggcg atatttattg ctgtcgcttg ctcccgccag
    26461 tttgttggca ctgatgacgt ggaaaccgtg gctggcgaaa tcgagatact gatcagcaag
    26521 ctgctggctg gcggtaacgt ccaacaccac taaatcatca tacggatggg cgcgcatcca
    26581 caggaaaagc gactcttcat cctgctcgac cgcttcatcg ttgaagaagg ctaacgcgcg
    26641 gctggcgtcc agcccgtcat aactcaacag actgcggcgg ctgtccacca cgcctgccag
    26701 cacaaattca aagccggtac gcgccgaaag cgtgctctgc tcgcgggcga acagttccag
    26761 ccagcgagag ccgatattgc ctttgccgaa caataccagg ccgatgcgtt tttctgcgcg
    26821 gaagacggac tgatgcagcc cctgaatcag gctttcggtc gggccggtgc gcagtactgc
    26881 caccagactg atgccgtcat cggactgcca cgtaaactcg accggctggc ctttcagttg
    26941 ctgccagaag cggtggcaat gcagcgggtt acgggtgaca cccgccccga ccatcgccac
    27001 cagcgccagc ccctgacgca ggcgcagttc tccaggtaat cccgcttcgt cgaggatttt
    27061 cagcgcactg tcggccactt ctgaggtgta gcaaaattgc aacaactggc gatcgttatg
    27121 cacgccaacc gccagtggac gcacctgggc acgtttcagg atctggtcga tctctttatg
    27181 cgccagtttg aaatcctgac tggcgggcac ctgaaactca atcaaacaga catcatcgtg
    27241 gctggtgaca atacgcgcac cagtaccgga ggccagcacg cgttcaatgc gcgtggaacc
    27301 ttgatccggc gtgtagctac agcgcagctg caggtcgatt tcgctgccag aaactggctg
    27361 taaagtacgg gcgtgaagaa cgggagccgc cagacgcgcc agttcgctgg cctcatccag
    27421 acgcagcaac ggcagcaggc aggcatcttt cactttacgc gggtcggcac tgtatacccc
    27481 ggcgacgtcg ctccagatgg ttacacgaga aacacccgcc agcgcaccga tttgtgtcgc
    27541 ggaatagtcg gaaccgttac gccccagcaa caccgtttca ccggcgttgt tacggctgat
    27601 aaacccggtc accaccagac gtttgctcgg atgttgtacc agtagctgtt gcagcaacgg
    27661 gtaagaaagc ccttcatcaa cctgcggttg cgcagcgcgt tcagcgcgta aaaactcacg
    27721 ggcatcaagc caggccgctg gcagcccttg ttgattaagt accgcagaca tcaggcgcgc
    27781 cgaccacact tcaccgtggc ccaccacttc cgcatacacc gcatcgttaa taccactgtc
    27841 gagcaacgcc gccagacgct caagatcgct gacaaaagcg ctaatgaggc tatcggcttc
    27901 ttcagcgggt agcagaccgc taatcagatc gcactgatag cgacgcagcg tttgttgaac
    27961 ctgatgcgca gagagacgat cggtctggct tagtttcaac cagttaatca actggttagt
    28021 ggtgctaccg gcggcggaaa ccaccatcat atcgtcaggc tgagagaact ccgccataat
    28081 gcccgcgaca cgcaaataac acttcacatc agccagacta ctgccaccaa atttatgcag
    28141 ctgacgacct ttcgcccctg cctgcgcaat cacactcatt tttacccctt gtttgcagcc
    28201 cggaagccat tttccaggtc ggcaattaaa tcttcgccat cttcaatacc ggtggagata
    28261 cgcagcagcg tctcggagat cccggcggca gcacgcgctt ctggtgccat gcctgcatgt
    28321 gtcatggttg cggcgtgaga gattaaactt tccactcccc ctaatgattc cgccagcgta
    28381 aacaacgaca gcccgcccag gaaacgacac agcgtctgct catcgccatc cagttcaaaa
    28441 ctcaacattg cgccaaagcc tttttgctga cgcgcggcaa tttcatgccc ctgattttcc
    28501 ggcagcgacg ggtgatacag ttttttcacc aacggctggg tttgcagata tttcacaatc
    28561 gcctgcgcgt tgcgctgcgc cagctccata cgcggcacca gcgttcgcaa cccgcgtagc
    28621 agcagatagc tgtcaaacgc gccgcccgtc acgccaatat tgtttgccca ccaggcaagt
    28681 tcagtgacaa cgtccgggtc tttagcaatc accacgccag ccaccacgtc agagtgaccg
    28741 ttcagatatt tcgtacatga atgcaacacc agatcggcac ccaatgccag cggattttgt
    28801 aatgccgggc ttaagaaggt gttatccacc acgctcaccg ccccgacttc ccttgccaga
    28861 tggcagattt tcgcaatatc cacgacgcgt aacaatggat tacttgggct ttctaccagt
    28921 accagtttgg gtttttctgc cagcgctgcc cgtaatgcct gttcatcgcc ttgatcaaca
    28981 aacaacacgc gatagcaacc gcgtttcgcc agactgtcga acaggcgata gctaccgccg
    29041 tagcagtcgt gcggcgcaac cagcagatcg ccaggtttca aaaagacggt cgttaccagg
    29101 tgaatcgcgg acatgccggt attagtaagt actgcgccag caccgccttc cagttctgcc
    29161 agcgcacgct gaaccacatc gcgcgttggg ttgccgcgac gcgagtaatc atgcgcgcgc
    29221 ggttcattaa atccggtaaa gttataggtg ctggaaagat ggatgggagg gacaacgcaa
    29281 ccatactgtt cgtcgtcatt taacccgcta cgcactgcga tggtggcctg tttacgcgtc
    29341 atgtgatgaa gtttcctggg ctttgtcggt gaaatgtcag gcaccagagt aaacattgtg
    29401 ttaatggacg tcaatacatc tggacatcta aacttctttg cgtatagatt gagcaaatcc
    29461 caaatagccg ttaaaattat atgcattatc acgccgacag gtgcattaca cgatgtcacg
    29521 gtaacgcctg tacggtaaac tatgcgggtt tacggtcagt acccacataa attgtggggt
    29581 ctggtctcaa tttattgacg aagaggatta agtatctcat ggctgaatgg agcggcgaat
    29641 atatcagccc atacgctgag cacggcaaga agagtgaaca agtcaaaaag attacggttt
    29701 ccattcctct taaggtgtta aaaatcctca ccgatgaacg cacgcgtcgt caggtgaaca
    29761 acctgcgtca cgctaccaac agcgagctgc tgtgcgaagc gtttctgcat gcctttaccg
    29821 ggcaaccttt gccggatgat gccgatctgc gtaaagagcg cagcgacgaa atcccggaag
    29881 cggcaaaaga gatcatgcgt gagatgggga ttaacccgga gacgtgggaa tactaaagcg
    29941 gaaaagagcg gcgcggagtg gaatcgcctg atgcactacg cttatcaggc ctacgtcata
    30001 ttgcaattta ttgaatttgc acgaacttgt aggccggata aggcgttcac gccgcatccg
    30061 gcataaacaa cgagcacgtt gtctgcgacc caccgctttt tatacatgga cgtttaacta
    30121 tgaaaaacag gctgctgatc ctcagcctgc tggtttctgt acctgccttt gcctggcagc
    30181 cgcaaactgg cgacatcatc tttcaaatct ctcgctcatc gcaaagtaaa gcgatccaac
    30241 tggcaaccca ttccgattac agccacaccg gtatgctggt gatgcgtaac aaaaagccct
    30301 acgtttttga agcggtcggc ccggtgaaat acaccccgct caagcagtgg attgcccatg
    30361 gtgaaaaggg caactacgtt gttcgccgcg ttgaaggcgg actgagtgtt gaacaacagc
    30421 aaaaactgac gcaaacggca aaacgttatc tcggtaaacc gtatgatttc agcttctcat
    30481 ggagcgacga tcgccagtac tgttcggaag tggtgtggaa ggtttaccag aacgcgctgg
    30541 gaatgcgcgt gggcgaacag cagaagttga aagagtttga tctcagcaac ccgctggtcc
    30601 aggcgaagct caaagaacgc tacggtaaaa atattccgct tgaggaaacg gtagtttcac
    30661 cacaagccgt tttcgacgcg ccacaactca ccacggttgc caaagagtgg ccgctgtttt
    30721 cgtggtgata cgaccaaaat tcaggcacaa aaaaagcgcc gtgcggcgct tttttcggaa
    30781 atccggtctt atttgctgcc cgggatgttg aaacgcttgt tgaagcggtc aacacggcca
    30841 ccggtagcaa catcacgctg tttgccagtg aagaacgggt ggcacttgct gcacacgtcg
    30901 aggttcaggt catgaccaac ggtggagcgg atttttatta cgttaccgca agagcagcta
    30961 gcagtaattt cttcgtattt cgggtgaata tcttttttca tgggaaaacc tcggtttaag
    31021 gccgcgtcgc tcttccagcc ctaacgccag acaccacgcg atgttaaaag tatagcttca
    31081 atacgatcat ttcgtacgaa ggcgcgaaat catacagaaa ttaaccagcg tatgcaaact
    31141 gatccgcact cttctacggc aatgtgtata ctaacccacc gaatttcaag tcaggatgat
    31201 gctatgcccg ttgcccacgt tgccttgccc gttccgcttc ctcgtacctt tgactatctg
    31261 ctgccagaag gcatgacggt taaagctggg tgtcgcgtgc gcgtgccgtt tggcaaacag
    31321 caggagcgca tcgggattgt ggtatcagtt agcgatgcca gcgaactgcc gctcactgag
    31381 ctaaaagcgg tagtcgaagt gctggatggt gagccggtgt ttactcactc cgtctggcga
    31441 ttgctgctat gggcggcaga ttactatcat catccgattg gcgatgtgct gtttcatgcc
    31501 ttgccgattt tactacgcca ggggcggcct gcggcgaacg cgccgatgtg gtactggttt
    31561 gccactgaac aaggtcaggc ggtggatctg aacagcctga aacgctcacc caagcaacaa
    31621 caggcgctgg cagcgttacg gcaaggcaaa atctggcgcg accaggttgc cacgctcgaa
    31681 tttaatgatg ccgcgttgca ggcgctgcgc aaaaaaggtc tgtgtgattt agcaagtgaa
    31741 acaccagagt ttagcgactg gcgaacgaac tatgccgttt ctggtgagcg gttgcgattg
    31801 aataccgaac aggccaccgc cgttggcgca attcatagcg cggcagatac tttttctgcc
    31861 tggctgctgg cgggcgttac cggttccggt aaaacggagg tttatctcag tgtactggaa
    31921 aacgtgctcg ctcagggcaa acaggcgctg gtgatggtgc cggaaatcgg cctgacaccg
    31981 caaactatcg cccgttttcg tgaacggttt aatgcgcccg tggaagttct gcattccggt
    32041 ctgaacgaca gcgagcgtct ttcggcgtgg ctgaaagcga aaaatggcga ggcggcgatt
    32101 gtgatcggca cccgctccgc gctgtttacg ccgtttaaaa atctcggcgt gattgtcatt
    32161 gacgaagagc acgacagttc ctacaagcag caggaaggct ggcgctatca tgcccgcgac
    32221 ctggcggtgt atcgtgcgca tagcgagcaa atcccgatta ttcttggctc cgcaacgccc
    32281 gcgctggaaa cgttgtgcaa cgtccagcag aaaaaatacc gcctgctgcg cctgacgcgc
    32341 cgggcgggga atgcgcgtcc ggcaattcaa cacgtgctgg atttaaaagg tcagaaggtg
    32401 caggcaggtc tggctccggc gttaatcacc cgtatgcgcc agcatttaca ggccaacaat
    32461 caggttatcc tctttcttaa ccgccgtggc tttgcgcctg cgctactgtg ccacgactgc
    32521 ggctggattg ccgaatgccc acgttgcgat cactactaca cgctgcatca ggcgcaacag
    32581 catttgcgct gtcaccactg tgacagtcag cgtccggtgc cgcgccagtg cccttcctgc
    32641 ggttccacgc acctggtacc cgtggggctg ggcaccgaac agcttgaaca gacgcttgcg
    32701 ccgctgttcc ctgatgtgcc catttctcgt atcgaccgcg ataccaccag ccgcaaaggg
    32761 gcgctggaac agcaactggc ggaagtacat cgcggcggtg cgaggatttt gatcggcaca
    32821 caaatgctgg cgaaaggtca ccactttccg gatgtgacgc tggtggcgtt actggatgtg
    32881 gacggcgcgc tgttttctgc cgattttcgc tcggcagagc gtttcgctca gctttacacc
    32941 caggtcgctg gtcgtgccgg gcgtgcgggt aaacaaggcg aagtggtgct gcaaacgcac
    33001 catccggaac atcctctgtt gcaaacgttg ctctataaag gctacgatgc ctttgccgaa
    33061 caggcgctgg ctgagcgacg gatgatgcaa ctgccgccat ggaccagcca tgtgattgtg
    33121 cgtgcggaag atcataacaa tcagcatgcg ccgctgttcc tgcaacaact gcgtaatctg
    33181 atcctctcca gcccgctggc agacgacaaa ctgtgggttc tcggtccggt tccggcactg
    33241 gcacctaaac gtggcggtcg ctggcgctgg cagatattgt tgcagcaccc ttcccgcgtg
    33301 cgcttgcaac acatcattag tggtacgctg gcgctcatca atacaatacc ggattcccgc
    33361 aaggtgaaat gggtactgga tgttgatccg attgagggtt aaaccgctta cgatgcgagg
    33421 cggatcgaaa aattcaatat tcatcacact tttcatgaaa attctgtaac cgttttcacg
    33481 cgctatctgc taaaaatgtt gccgatgtga agtaaacatg gatgtagtac gcctgacgtg
    33541 ccaggcgagg agtgagtgtg aaagcgaaga agcaggaaac tgccgcgacc atgaaagacg
    33601 ttgccctcaa ggcaaaagtc tctacagcga ctgtctcccg agcattaatg aatcccgata
    33661 aagtctccca ggccacccgt aatcgggttg aaaaagcggc ccgggaagtg ggttatttac
    33721 cgcagcctat ggggcgcaac gtcaagcgta atgaatcccg caccattctg gtgattgtcc
    33781 cggatatctg cgatcccttc tttagcgaaa ttattcgcgg tatcgaagtt acggcggcaa
    33841 atcacggata tctggtgctg attggcgact gtgcgcatca aaatcagcag gaaaaaacct
    33901 ttatcgattt gatcatcacc aagcaaattg atggcatgtt gctgctgggt tcaaggctgc
    33961 cgtttgatgc cagcattgag gaacagcgta atctgccgcc gatggtgatg gcgaacgaat
    34021 ttgcaccgga actggagctg cctaccgttc atatcgacaa tctgaccgcc gcatttgatg
    34081 cagtaaatta tttatatgag caagggcata aacggattgg ctgtatagcc ggtcccgaag
    34141 agatgccact gtgtcactac cgcctgcaag gctatgttca ggcgctgcgt cgctgcggaa
    34201 ttatggttga tccacaatac atcgcccgtg gcgacttcac cttcgaagcc ggaagcaaag
    34261 caatgcaaca gctgcttgat cttccacaac cgcctactgc agtcttctgc catagcgatg
    34321 tgatggcgct cggcgcactt tctcaggcaa aacgccaggg gctgaaagtc ccggaagacc
    34381 tttccataat cggttttgat aacatcgacc tgacgcaatt ttgtgatccg ccgctgacaa
    34441 ccatcgcgca gccgcgttac gaaatcggtc gggaagctat gctgttattg cttgatcaaa
    34501 tgcaggggca acacgttggc agtggctctc gtttaatgga ctgcgaactt atcatccggg
    34561 gatcaacacg cgcgttacct taaagtaaac ctttaagact gccgtgactg gtcaaaggcc
    34621 cgccgcttaa gtaacatggc gggctgacga acgaataaat acagcgaaac gatagtggca
    34681 caacgagatt atgtacgccg cagccaaccg gcaccttcgc ggcgaaaaaa gagcacctca
    34741 cggaaaaagc aacgaaatct gcctgcggtt tctcccgcta tggtcgctat tgctgccgcc
    34801 gttcttgtga cctttatcgg tggtctgtac ttcattacgc atcacaagaa agaagagtcc
    34861 gagacgctgc aaagccagaa agtgaccgga aacggactac caccaaaacc agaagaacgc
    34921 tggcgctaca ttaaagagct ggaaagtcgc cagccgggtg tgcgtgcgcc cacagaacct
    34981 tctgccggtg gtgaagtgaa aacgccggag caactgacac cagaacaacg tcagcttctt
    35041 gaacaaatgc aggctgatat gcgccagcag ccaacgcagc tggttgaggt tccgtggaat
    35101 gaacagacgc cggaacagcg tcagcaaacg ctacaacgcc agcgtcaggc gcagcaatta
    35161 gcggagcagc aacgcctggc tcagcagtcg cgtacgactg aacaaagctg gcagcagcag
    35221 acgcgtacgt cgcaagccgc gccggtacag gcgcaaccgc gccagtctaa accagcttct
    35281 actcagcagc cgtaccagga tctgctgcaa acccctgcgc acacgaccgc gcagtcgaag
    35341 ccgcagcaag ctgcgccggt tgctcgtgcc gctgacgcgc caaaaccgac ggcggagaaa
    35401 aaagacgaac gccgctggat ggtgcagtgc ggttcgttca gaggcgcgga acaggcagag
    35461 acggtacgtg ctcagctggc gttcgaagga tttgactcga aaatcaccac caacaatggc
    35521 tggaatcgtg tggtcattgg cccggtgaaa ggcaaagaga acgcagacag caccctcaat
    35581 cggttgaaga tggcgggtca tacaaactgc attcggctcg ccgccggggg ttgaaaccct
    35641 caaaatcccc cccatctata attgcattat gccccgtact tttgtacggg gtttgtactc
    35701 tgtattcgta accaaggggt cagctcgtga caactatagt aagcgtacgc cgtaacggcc
    35761 atgtggtcat cgctggtgat ggtcaggcca cgttgggcaa taccgtaatg aaaggcaacg
    35821 tgaaaaaggt ccgccgtctg tacaacgaca aagtcatcgc gggctttgcg ggcggtactg
    35881 cggatgcttt tacgctgttc gaactgtttg aacgtaaact ggaaatgcat cagggccatc
    35941 tggttaaagc cgccgttgag ctggcaaaag actggcgtac cgatcgcatg ctgcgcaaac
    36001 ttgaagcact gctggcagtc gcggatgaaa ctgcatcgct tatcatcacc ggtaacggtg
    36061 acgtggtgca gccagaaaac gatcttattg ctatcggctc cggcggccct tacgcccagg
    36121 ctgcggcgcg cgcgctgtta gaaaacactg aacttagcgc ccgtgaaatt gctgaaaagg
    36181 cgttggatat tgcaggcgac atttgcatct ataccaacca tttccacacc atcgaagaat
    36241 taagctacaa agcgtaagga tctcccatgt ctgaaatgac cccacgcgaa atcgtcagcg
    36301 aactggataa gcacatcatc ggccaggaca acgccaagcg ttctgtggcg attgctctgc
    36361 gtaaccgctg gcgtcgcatg cagctcaacg aagagctgcg ccatgaagtg accccgaaaa
    36421 atatcctgat gatcggcccg accggtgtcg gtaaaactga aatcgcccgt cgtctggcta
    36481 agctggcgaa tgcgccgttc atcaaagttg aagcgaccaa atttaccgaa gtgggctacg
    36541 tcggtaagga agttgattct attattcgcg atctgaccga tgccgccgtg aaaatggtac
    36601 gcgtccaggc tatcgagaaa aaccgttatc gcgctgaaga actggcagaa gaacgtattc
    36661 tcgacgtgct gatcccacct gctaaaaaca actggggaca gactgaacag cagcaggaac
    36721 cttccgctgc tcgtcaggca ttccgcaaaa aactgcgtga aggccagctt gatgacaaag
    36781 aaatcgagat cgatcttgcc gcagcaccga tgggcgttga aattatggct cctccgggca
    36841 tggaagagat gaccagccag ctgcagtcca tgttccagaa cctgggcggc cagaagcaaa
    36901 aagcgcgtaa gctgaaaatc aaagacgcca tgaagctgct gattgaagaa gaagcggcga
    36961 aactggtgaa cccggaagag ctgaagcaag acgctatcga cgctgttgag cagcacggga
    37021 tcgtgtttat cgacgaaatc gacaaaatct gtaagcgcgg cgagtcttcc ggtccggatg
    37081 tttctcgtga aggcgttcag cgtgacctgc tgccgctggt agaaggttgc accgtttcca
    37141 ccaaacacgg gatggtcaaa actgaccaca ttctgtttat cgcttctggc gcgttccaga
    37201 ttgcgaaacc gtctgacctg atcccggaac tgcaaggtcg gctgccaatc cgcgttgaac
    37261 tgcaggcact gaccaccagc gacttcgagc gtattctgac cgagccgaat gcctctatca
    37321 ccgtgcagta caaagcactg atggcgactg aaggcgtaaa tatcgagttt accgactccg
    37381 gtattaaacg catcgcggaa gcggcatggc aggtgaacga atctaccgaa aacatcggtg
    37441 ctcgtcgttt acacactgtt ctggagcgtt taatggaaga gatttcctac gacgccagcg
    37501 atttaagcgg tcaaaatatc actattgacg cagattatgt gagcaaacat ctggatgcgt
    37561 tggtggcaga tgaagatctg agccgtttta tcctataatc gcgttcaatc attttcatca
    37621 ttgtttgatg gggctgaaag gccccatttt tattggcgcg tattatgact gaacaacaaa
    37681 ttagccgaac tcaggcgtgg ctggaaagtt tacgacctaa aaccctcccc ctcgcctttg
    37741 ctgcaattat cgtcgggacg gcgctggcat ggtggcaagg tcacttcgat ccgctggtcg
    37801 ccctgctggc actaattacc gccgggctat tacagatcct ttctaacctc gccaatgatt
    37861 acggcgatgc ggtaaaaggc agcgataaac ctgaccgcat tgggccgcta cgcggcatgc
    37921 aaaaaggggt cattacccag caaaagatga aacgggcgct cattattacc gtcgtgctca
    37981 tctgtctctc cgggctggca ctggttgcag tggcatgcca tacgctggcc gattttgtcg
    38041 gtttcctgat tcttggcggg ttgtcgatca ttgccgctat cacctacacc gtgggcaatc
    38101 gtccttatgg ttatatcggt ctgggtgata tttccgtact ggttttcttt ggctggttga
    38161 gtgtcatggg gagctggtat ttacaggctc atacattgat tccggcactg atccttccgg
    38221 cgaccgcatg cggcctgctg gcaacggcag tactgaatat taataacctg cgtgatatca
    38281 atagcgaccg cgaaaatggc aaaaacacgc tggtggtgcg cttaggtgaa gtgaacgcgc
    38341 gtcgttatca tgcctgcctg ctgatgggct cgctggtgtg tctggcgctg tttaatctct
    38401 tttcgctgca tagcctgtgg ggctggctgt tcctgctggc ggcaccatta ctggtgaagc
    38461 aagcccgtta tgtgatgcgg gaaatggacc cggtggcgat gcgaccaatg ctggaacgta
    38521 ctgtcaaggg agcgttactg actaacctgc tgtttgtttt agggatattc ctaagccagt
    38581 gggcagcata actgacaaat atcaattaac aattgatgat tttgccaaca gcccacatag
    38641 cgcgatatac tgaaaattct cgcagcaact gaatgttaag cctatgaaat acgatacttc
    38701 cgagctttgt gacatctatc aagaagatgt taacgtcgtg gaaccgctgt tctccaactt
    38761 tggcggacgg gcgtcgtttg gcggacaaat aatcacggta aaatgtttcg aggacaacgg
    38821 gttgctgtac gatctgctcg aacagaatgg ccgtggtcgt gttcttgtcg tcgatggcgg
    38881 tggttctgtt cgtcgcgcac tggtcgatgc tgaactggcg cgtctggcag tacaaaatga
    38941 atgggaaggt ctggtcattt acggcgcggt gcgtcaggta gatgacctgg aagagttgga
    39001 tatcggcatc caggcgatgg cggcaattcc ggttggtgcc gctggcgaag gcattggcga
    39061 aagcgatgtc cgcgtcaatt ttggcggtgt caccttcttc tccggcgacc atctttatgc
    39121 cgacaatacc gggattattc tttcagaaga tccgctggat attgaatgat aagaaaggca
    39181 ccgcaaggtg ccttttttct gcgttacctg ttggcctaca cagtaaagaa attacgcgaa
    39241 agatgaagcg taatcagacc tcttccatgc gacccagcag ggcctgcaga cgttcctgcc
    39301 agccgttctg ctgttctttc agatggttgt tctcacgctc cagctcttcg cgctgatgct
    39361 gggcattttg aacttcctgc gacagtgagt tgtttttttc tttcagctct tcgatttcca
    39421 tctgcaacag agtgatggta tcaatcgcct gctgtacttt tgcttccagt ttctcaaaca
    39481 cttctaatga cattgtcata cctctcctga attgcaaggc gttgatggat aaaaaatcct
    39541 cgtcccgatt accggtgacg ccttaataaa tacgagcgca ctttagttag ctccgattgt
    39601 atgaagccgc gccatcgctg tccagcggca cgccttgcag attacggttt gccacacttt
    39661 tcatccttct cctagtgaca taatccacat caatcgaaaa tgttaataaa tttgttgcgc
    39721 gaatgatcta acaaacatgc atcatgtaca atcagatgga ataaatggcg cgataacgct
    39781 cattttatga cgaagcacac acattttaag ttcgatattt ctcgtttttg ctcgttaacg
    39841 ataagttaac agcatgccta caagcatcgt ggaggtccgt gactttcacg catacaacaa
    39901 acattaactc ttcaggatcc gattatgagt caaacatcaa ccttgaaagg ccagtgcatt
    39961 gctgaattcc tcggtaccgg gttgttgatt tttttcggtg tgggttgcgt tgcagcacta
    40021 aaagtcgctg gtgcgtcttt tggtcagtgg gaaatcagtg tcatttgggg actgggggtg
    40081 gcaatggcca tctacctgac cgcaggggtt tccggcgcgc atcttaatcc cgctgttacc
    40141 attgcattgt ggctgtttgc ctgtttcgac aagcgcaaag ttattccttt tatcgtttca
    40201 caagttgccg gcgctttctg cgctgcggct ttagtttacg ggctttacta caatttattt
    40261 ttcgacttcg agcagactca tcacattgtt cgcggcagcg ttgaaagtgt tgatctggct
    40321 ggcactttct ctacttaccc taatcctcat atcaattttg tgcaggcttt cgcagttgag
    40381 atggtgatta ccgctattct gatggggctg atcctggcgt taacggacga tggcaacggt
    40441 gtaccacgcg gccctttggc tcccttgctg attggtctac tgattgcggt cattggcgca
    40501 tctatgggcc cattgacagg ttttgccatg aacccagcgc gtgacttcgg tccgaaagtc
    40561 tttgcctagc tggcgggctg gggcaatgtc gcctttaccg gcggcagaga cattccttac
    40621 ttcctggtgc cgcttttcag ccctatcgtt ggcgcgattg taggtgcatt tgcctaccgc
    40681 aaactgattg gtcgccattt gccttgcgat atctgtgttg tggaagaaaa ggaaaccaca
    40741 actccttcag aacaaaaagc ttcgctgtaa tatgactacg ggacaattaa acatgactga
    40801 aaaaaatata tcgttgcgct cgaccagggc accaccagct cccgcgcggt cgtaatggat
    40861 cacgatgcca atatcattag cgtgtcgcag cgcgaatttg agcaaatcta cccaaaacca
    40921 ggttgggtag aacacgaccc aatggaaatc tgggccaccc aaagctccac gctggtagaa
    40981 gtgctggcga aagccgatat cagttccgat caaattgcag ctatcggtat tacgaaccag
    41041 cgtgaaacca ctattgtctg ggaaaaagaa accggcaagc ctatctataa cgccattgtc
    41101 tggcagtgcc gtcgtaccgc agaaatctgc gagcatttaa aacgtgacgg tttagaagat
    41161 tatatccgca gcaataccgg tctggtgatt gacccgtact tttctggcac caaagtgaag
    41221 tggatcctcg accatgtgga aggctctcac gagcgtgcac gtcgtggtga attgctgttt
    41281 ggtacggttg atacgtggct tatctggaaa atgactcagg gccgtgtcca tgtgaccgat
    41341 tacaccaacg cctctcgtac catgttgttc aacatccata ccctggactg ggacgacaaa
    41401 atgctggaag tgctggatat tccgcgcgag atgctgccag aagtgcgtcg ttcttccgaa
    41461 gtatacggtc agactaacat tggcggcaaa ggcggcacgc gtattccaat ctccgggatc
    41521 gccggtgacc agcaggccgc gctgtttggt cagttgtgcg tgaaagaagg gatggcgaag
    41581 aacacctatg gcactggctg ctttatgctg atgaacactg gcgagaaagc ggtgaaatca
    41641 gaaaacggcc tgctgaccac catcgcctgc ggcccgactg gcgaagtgaa ctatgcgttg
    41701 gaaggtgcgg tgtttatggc aggcgcatcc attcagtggc tgcgcgatga gatgaagttg
    41761 attaacgacg cctacgattc cgaatatttc gccaccaaag tgcaaaacac caatggtgtg
    41821 tatgtggttc cggcatttac cgggctgggt gcgccgtact gggacccgta tgcgcgcggg
    41881 gcgattttcg gtctgactcg tggggtgaac gctaaccaca ttatacgcgc gacgctggag
    41941 tctattgctt atcagacgcg tgacgtgctg gaagcgatgc aggccgactc tggtatccgt
    42001 ctgcacgccc tgcgcgtgga tggtggcgca gtagcaaaca atttcctgat gcagttccag
    42061 tccgatattc tcggcacccg cgttgagcgc ccggaagtgc gcgaagtcac cgcattgggt
    42121 gcggcctatc tcgcaggcct ggcggttggc ttctggcaga acctcgacga gctgcaagag
    42181 aaagcggtga ttgagcgcga gttccgtcca ggcatcgaaa ccactgagcg taattaccgt
    42241 tacgcaggct ggaaaaaagc ggtcaaacgc gcgatggcgt gggaagaaca cgacgagtaa
    42301 tgtcaggcac ctgaatcgat tgcctgatgc tgatcgcctg atgcgacgct tacgcgtctt
    42361 atcaggccta cgagccaatg ccattttgtg taggcgggat aaggcgttca cgccgcatcc
    42421 ggcatcacgc agactctgaa accgtgcggg ggcaaccccg cacacatcaa taatcccacc
    42481 cttcccctgt gctacacttc gcgccattcc ttactgctta gagtttgcta tgagacgaga
    42541 acttgccatc gaattttccc gcgtcaccga atctgcggcg ctggctggct acaaatggtt
    42601 aggacgcggc gataaaaaca ccgcggacgg cgcggcggta aacgccatgc gtattatgct
    42661 caaccaggtc aacattgacg gcaccatcgt cattggtgaa ggtgaaatcg acgaagcacc
    42721 gatgctctac attggtgaaa aagtcggtac tggtcgcggc gacgcggtag atattgctgt
    42781 tgatccgatt gaaggcacgc gcatgacggc gatgggccag gctaacgcgc tggcggtgct
    42841 ggcagtaggc gataaaggct gcttcctcaa tgcgccggat atgtatatgg agaagctgat
    42901 tgtcgggccg ggagccaaag gcaccattga tctgaacctg ccgctggcgg ataacctgcg
    42961 caatgtagcg gcggcgctcg gcaaaccgtt gagcgaactg acggtaacga ttctggctaa
    43021 accacgccac gatgccgtta tcgctgaaat gcagcaactc ggcgtacgcg tatttgctat
    43081 tccggacggc gacgttgcgg cctcaattct cacctgtatg ccagacagcg aagttgacgt
    43141 gctgtacggt attggtggcg cgccggaagg cgtggtttct gcggcggtga tccgcgcatt
    43201 agatggcgac atgaacggtc gtctgctggc gcgtcatgac gtcaaaggcg acaacgaaga
    43261 gaatcgtcgc attggcgagc aggagctggc acgctgcaaa gcgatgggca tcgaagccgg
    43321 taaagtattg cgtctgggcg atatggcgcg cagcgataac gtcatcttct ctgccaccgg
    43381 tattaccaaa ggcgatctgc tggaaggtat tagccgcaaa ggcaatatcg cgactaccga
    43441 aacgctgctg atccgcggca agtcacgcac cattcgccgc attcagtcca tccactatct
    43501 ggatcgcaaa gacccggaaa tgcaggtgca cattctctga gtgatttgat cgattgagcc
    43561 ttccagtcct tcgggactgg aatttttttg ttcggagaac gaagataagg caagtcaatc
    43621 aaaacaggag aaaaacatgg ctgattgggt aacaggcaaa gtcactaaag tgcagaactg
    43681 gaccgacgcc ctgtttagtc tcaccgttca cgcccccgtg cttccgttta ccgccgggca
    43741 atttaccaag cttggccttg aaatcgacgg cgaacgcgtc cagcgcgcct actcctatgt
    43801 taactcgccc gataatcccg atctggagtt ttacctggtc accgtccccg atggcaaatt
    43861 aagcccacga ttggcggcac tgaaaccggg cgatgaagtg caggtggtta gcgaagcagc
    43921 tggcttcttt gttctggatg aagtaccgga ctgcgaaacg ctatggatgc tggcaaccgg
    43981 tacagcgatt ggcccttatt tatcgattct gcaactaggc aaagatttag atcgcttcaa
    44041 aaatctggtc ctggttcacg ccgcacgtta tgccgccgac ttaagctatt tgccactgat
    44101 gcaagaactg gaaaaacgct acgaaggcaa actgcgcatt cagacggtgg tcagtcggga
    44161 aacggcagcg gggtcgctca ccggacggat accggcatta attgaaagtg gggaactgga
    44221 aagcgcgatt ggcctgccga tgaataaaga aaccagccat gtgatgctgt gcggcaatcc
    44281 acagatggtg cgcgatacac aacagttgct gaaagagacc cggcagatga cgaaacattt
    44341 acgtcgccga ccgggccata tgacagcgga gcattactgg taagcggtta cttatcgata
    44401 aacggcacga tgagcaaatc cgcactcatc ttattgatca tcccgcgata tgccggcatc
    44461 aaacggttga taaatgagtg atgatgacca cagacaagga ggtcgcactg ctctttttgc
    44521 ataatttcca gcagtgtttc cggcatttct ccgcgttcaa tacgcagttt tgtcttcggc
    44581 cattgaatat ttttcgtcag tttatacagc ttgttatccg acttattctt caacaattga
    44641 agaatatctt ctgttgcagg gaagtagata cccggataca actcgcttaa gccatcatca
    44701 atatgaatta acgtcaggtg agcgtcatta tgtctggcga gctccagggc tttatttacc
    44761 agtaaggcat cttcttcatt cccggaaatt gccacgccaa tgtgtttata agccatgttt
    44821 aactccttct aaagcaactc catcaagcta acgaacgcag tgataactca aaaataatca
    44881 tctcggcctg gcatgagaaa gtgaaggccg catcaagctc aacttgcccc tccagatcct
    44941 taatcttata ggccacgctg gccccttcat ctgcagagga tgcagcggcg gcaagctctt
    45001 tcagtttggt cataaaagac tctgcttctt cgcgggtggc aaaaaagcgc gaaaacttac
    45061 tggtacagtt gtcgttatca atcaccgtac cgatatcaat cgcacatcct ttagtactgc
    45121 atttatctac gacatctttc atagggcacc tctgtatatt cgcctctctg tcgattacga
    45181 cttaagaata atgcgccgtt aagggaagaa caaacaagta atgatcttat ggattaccat
    45241 tttttttgat attgcttaag gtgaagagtt cagaaggatt ttccgggtat tgtcatttgc
    45301 atcgtggaaa taacaatccc ggaaaaaaat agcagtaaga gatatattaa aggttaatta
    45361 cttgatttat tgtcggcttt atacttcaca tcctgagtat ctttaccata tttattttcg
    45421 ccttgtgtgc caacaaacgc accaagatcg ataagcatca tcaccagaat caacgtcggg
    45481 acaaaacgcc ccaccgccca ttgccagaca cccggtaaaa tcgctcagtt acccgccagc
    45541 agcatccacg ccacaatcat cagaaatgcc catgcgccgg aacgcccgcg atcatgcaag
    45601 cgcttaacag ttactgccgc tgttggccag agcaagcaca caaggcaaaa cgccgcggtc
    45661 tgaatatcga gtagattctt acccgccagt gagaaaagca ccagcatgcc tgcgaaccac
    45721 aggcctatcc aaatccagaa atcacggcgt ccaatacgcc ctttaaatga gaataaccat
    45781 tgctgtatgg tcatgtaagt tccttgatgg ttgtcttttc caggattcta cccttttgac
    45841 aagggcgaca gatatcgttt taatcggagc cagtcataac aaaaggtact gtcaatgaag
    45901 ccagggtgta cgctgttttt tctcttatgt tctgcattaa ccgttacaac aacggcgcat
    45961 gcacaaacac cagatacggc aacgaccgcg ccttatctgc tggctggagc ccctactttc
    46021 gatctctcca tcagccagtt tcgagaagac tttaacagcc agaatcccag cctgccactg
    46081 aacgaatttc gtgccatcga cagcagtccc gacaaagcca atctcactcg tgctgcaagt
    46141 aaaattaacg agaacttgta tgcttctaca gcgctggagc gcggtacctt aaaaatcaaa
    46201 agcattcaaa tgacctggct acccatccag gggccagagc aaaaagcagc gaaagcgaaa
    46261 gctcaggaat acatggcagc agtgatccgc acactcaccc cattaatgac caaaatacaa
    46321 agccagaaaa aactgcagtc gctactaacg gcggggaaaa acaaacgtta ttacaccgag
    46381 acagaaggtg cactgcgtta tgttgtcgcg gacaacggcg aaaaggggct gaccttcgct
    46441 gttgaaccga ttaagctggc gctatctgaa tcgcttgaag gtttgaataa atgacaaaaa
    46501 gcaaagcctt tgtgccgatg aatctctata ctgtttcaca gacctgctgc cctgcggggc
    46561 ggccatcttc ctttattcgc ttataagcgt ggagaattaa aatgcgacat cctttagtga
    46621 tgggtaactg gaaactgaac ggcagccgcc acatggttca cgagctggtt tctaacctgc
    46681 gtaaagagct ggcaggtgtt gctggctgtg cggttgcaat cgcaccaccg gaaatgtaca
    46741 tcgatatggc gaagcgcgaa gctgaaggca gccacatcat gctgggtgcg caaaacgtgg
    46801 acctgaacct gtccggcgca ttcaccggtg aaacttctgc cgctatgctg aaagacatcg
    46861 gcgctcagta catcatcatc ggccactctg aacgtcgtac ttaccacaaa gagtctgacg
    46921 aactgattgc gaaaaaattc gcggtgctga aagagcaggg tctgactccg gttctgtgca
    46981 tcggtgaaac cgaagctgaa aacgaagcgg gaaaaactga agaagtttgc gcacgtcaga
    47041 tcgacgcggt actgaaaact cagggtgctg cggcattcga aggtgcggtt atcgcttacg
    47101 aacctgtatg ggcaatcggt actggcaaat ctgcaactcc ggctcaggca caggctgttc
    47161 acaaattcat ccgtgaccac atcgctaaag ttgacgctaa catcgctgaa caagtgatca
    47221 ttcagtacgg cggctctgta aacgcgtcta acgctgcaga actgtttgct cagccagaca
    47281 tcgacggcgc gctggttggc ggtgcttctc tgaaagctga cgctttcgca gtgatcgtta
    47341 aagctgcaga agcggctaaa caggcttaag tctgacaggt gccggatttc atatccggca
    47401 cttactttcc ttaactcctc gccttaacgc aaaatctcac actcatgatc ctggatttcc
    47461 tcggctgaag cacggttcag cgtcagtaga tttcgttgtg tcgccagcaa cacaaacgaa
    47521 ttatcactct gccgtaccat cgccagcccg tagcttccca tatgttcccg cgcctcaggc
    47581 acttcttctg caagcatcat aaatgggctg cgttgtacca gttcgctttc cgttacccga
    47641 cgcgccaggt attcatgccc gcgcaaacca cctggcagtg gcaaccagcg gctgctgatg
    47701 ttcgccagat ttttatccag ttgttcgcgc acatcaggac gaatacaaga gatatgaata
    47761 tgaaaatggt tttgcgtatg cccggtgcgg gagttgatcg ccaaagaaac cgcgcgatcg
    47821 ggaaccggct ggccgtattt tttgctcata aaatcacgcg cctgccaggc caaccaaaag
    47881 aagttcggcg ttgaaggatc ggtcaacaaa ggactttcag taccgttaat acgatacgtt
    47941 ggcatcaaca gatattgcag tggaccatta agatctttta aaaccacgta tccggcattg
    48001 ggtttgactt ccgcacatgg cgaaggattt tgattttgct gctgattggg caaacattcc
    48061 tcaaggacaa tcttacgtaa tgtatccgac tcttcaccgg ttaatttcca gtaaccaata
    48121 ccggcagcca caacggcgat aactatcatc accaaaaaaa gaagacccgc ttttttcatc
    48181 tttttttccc tgtacctcaa agagatgcaa gggtaacgca aaatcgtgac aaataaaaaa
    48241 cccggtcgcg agaaaccacc gggtttgata attatcctag ggaaatcagc gtttgctgat
    48301 ctgatcgaac gtaccgccgt tagcaaaatg ctctttttgc gctttcgtcc agccgccgaa
    48361 ctcttcatca atggtgaata acttcagctt tggaaacgca ttttcgtact ttttcgctac
    48421 ttcagcgtcg cgcggacggt agtagttttt cgcggcaatt tcctgacctt ctggcgagta
    48481 gagatatttc aggtaggctt ccgccacctc tttggtgcct tttttctcga ccactttatc
    48541 gaccaccgac accgttggct ctgcgaggat agactcactc ggcgtgacga tttcgaattt
    48601 atctttcccc agttcattcg ctgccagcag agcttcgttt tcccaggcga tcagtacatc
    48661 gccaatcccg cgctcgacaa aggtgttagt ggagccgcgc gcgccagaat ccagaacttc
    48721 gacgttttta tacagtgccc gaacaaaatc ctgtgctttt gcctgatcgt tgttgttgtg
    48781 atgcagcgcg tagccccagg cagccaggta gttccagcgt gcgccaccag agcttttcgg
    48841 attaggcgtg atcaccgaaa cacccggttt aatcagatcg ttccagtcat ggatctgctt
    48901 cggattgccc ttacgcacca ggaaaacaat ggtggaagtg tacggtgcgg agttatccgg
    48961 cagacgtttg atccactctt tatcaatccg cccgcgttcc gcaatcgcgt ccacgtcata
    49021 ggccagagcc agcgtgacaa catcagcttc aataccgttg attaccgacg tcgcttgttt
    49081 acctgaacca ccgtgtgact gacgaatcac cacgttatca ccagtttgct gtttccagtg
    49141 ggcgctgaat gccttgttgt actgttcgta caattcgcgc gttggatcat atgaaacgtt
    49201 aagaagctga atatcctttg ccataacgct ggttgccgcc agcaaaaatg ttaaccctac
    49261 gccccacttg ttcatcgccc gactctctta tgttgtgttg tgatgagcaa agcgtgccag
    49321 aaggttaacc aaacattaaa gaataaaaaa agattggcta taacttgcgg gtatatgttg
    49381 agggattaaa aaggcggaag aaatccgcct catattgctg acaaagtgcg ctttgtccat
    49441 gccggatgcg gcgcgaacgc cttatccggc ctacaaaagt ttgcaaattc aataaattgc
    49501 agaattcatg taggcctgat aagcgaagcg catcaggcat ttttgcttct gtcatcggtt
    49561 tcaggctaaa ggaatctgcc tttttccgaa atcattaata cagttttttc gcgcagtcca
    49621 gccagtcacc tttgaacgga cgcttcatgt tttcgatagc gtcgatgatg tcgtggtgaa
    49681 ccagctgttc gttctggata cctacacaac gaccgccgta acctgccagc agcagatcga
    49741 tagcgtaagc gcccatacgg gaagccagaa tacggtcgta aggcaccgga gaaccaccgc
    49801 gctggatgtg gcccagcaca gttgcgcggg tttcacgacc ggtttctttc tcgatgaaat
    49861 gcgccagttc gtcaacatca cacatatgtt cggtaatcgc cacgatcgcg tgttttttac
    49921 ctttcgcgat acccgctttg atttcgttta ccaggtcttc acggctgaat tcaacttccg
    49981 gaactacaac gaattcacag ccaccggcaa tggccgcagc cagggtcaga tcgccacaat
    50041 aacggcccat cacttccacc acggaaatac gctggtgaga agaagaggtg tcacgcagac
    50101 ggtcgatcgc ttctacaacg gtgctcagcg cagtgaagaa accgatagtg tagtcagtgc
    50161 ctttgatgtc gttgtcgata gtgcccggca gaccgatgca cgggaagccc atttcggtca
    50221 gacgcattgc acccatgtag gaaccgtcac cgccgataac caccagcgcg tcgataccac
    50281 gttttttcag gttttcgata gccacggcgc ggatgttctc gtcgcggaat tccgggaaac
    50341 gcgcagaacc gaggaacgta ccgccacggt tgatcatgtc agacacgctg taacggtcta
    50401 gctgtaccat acggtcttca tacagaccca gatagccgtc ataaataccc attacttcca
    50461 gaccttctgt cagcgcagaa cgaacaaccc cgcgaattgc ggcgttcatg cctggcgcat
    50521 caccgccgct tgtcaacaca cctattttct taatcatgac tacctctgaa ctttggaatg
    50581 caaaatgaaa tctgttgccg gaagtcttct tgcacatcga agtgatccaa cgaatgtgca
    50641 aatagtataa caatcacttc ctgctgaatt gattcaggtc aggccaaatg gcggtatttt
    50701 atacacaaaa tgcgggtctg gctctctttt atactgatta tgaaagcata gaccgtttac
    50761 cctccctggg tacgacggaa caggggtcct gatggataat cacatccgat cccggaaaac
    50821 gccgtaaaat agcctgctct acctgatccg ccaccatatg tgcctgaacc aaaggcagag
    50881 agtcttccat ttccaaatga atctgaataa agcgggtcgg ccctgactgc cgcgtgcgaa
    50941 gatcgtgagc gccgctaaca cccggccagg aagtcacgat atcaataatt tcttgccgtt
    51001 cttcatcagg caatgcgcga tccagtaatg actgtaccgc ctcatatccc atgcgtaacg
    51061 cgctatataa aatatagatg ccgattccca atgcaaacag agcatcggcg cgatgccagc
    51121 cgtaccaaga caaccccagc gccagcagaa ttgcgccgtt catcataaca tcagactggt
    51181 aatgtagcat atcagcccgc accgcctggc tttgcgtccg gcgcaccacc caccgctgaa
    51241 acgagacaag gataatcgta caaattagcg ccacaattgt cacgataacc ccgacgcctg
    51301 gatctgtcat cggtgttgga gataccagat gttgaatacc cgtcaaaaac aggaatagtg
    51361 ccgaaccgga gataaacata ctttgcgcca gcgccgcgag ggactcagct ttaccgtgac
    51421 caaacgagtg attatcgtcg gcaggttgca gggaatatcg caccaccagt aaattcgtca
    51481 acgacgcgcc gatatccacc agcgaatcca ccagcgcggc gagaatactc accgacccgg
    51541 tataccacca tgcaaaaatt ttaatcagca atagcaacga agccatcgcc gtcgcagcaa
    51601 tcgccgcccg actgaccagc cgtccataag attgattcat aaatactccc gctatcaact
    51661 gacgctagta taacggaagc aaatcatctg caatgcatta agcagcaggc aaattgagga
    51721 taaaaaaaac cccacatcat gtgggggaag acagggatgg tgtctatggc aaggaaaaca
    51781 gggtttacta ctgggaacgt gagttgctac tactcaatag cttcaacgat gaactttttt
    51841 gccattgcgt cacgtcacgc aactgctcca ttcgttgttg atgtttctcg tttaaaaccg
    51901 cttgctgctc cggcgttaac aggcgataca tttggttgcg gacttttgcc atctcaacct
    51961 gacgagcaat ttgctcattc gccatttttt ctgcctgtgc gcgcacagcg ttttcatcaa
    52021 aattttctgc ggtgacaagg cgatgcattg tctccagttc gctaacatta acaggaggct
    52081 gttcgtgccg ggcctgttgc ataagatctc gcatctgctg acgctgatgt tcggttaaac
    52141 ttatgccgtc gaacatatgg ctctgcgtac tgcgctgcgt aagttcttca cccggatgcc
    52201 agttatcgcc tgaaccgact tcagcagcgt ggcttaatga actgactgcc agcgttgagg
    52261 ccatgacggc agcggtaact atgcgcatca tttgctccca aaatctttct gtcgcgattc
    52321 aacgatagag agtttacgat tcaggctgca aacatgcgtc agggggtgta aaacaacgta
    52381 aagtcatgga ttagcgacgt ctgatgacgt aatttctgcc tcggaggtat ttaaacaatg
    52441 aataaaatcc tgttagttga tgatgaccga gagctgactt ccctattaaa ggagctgctc
    52501 gagatggaag gcttcaacgt gattgttgcc cacgatgggg aacaggcgct tgatcttctg
    52561 gacgacagca ttgatttact tttgcttgac gtaatgatgc cgaagaaaaa tggtatcgac
    52621 acattaaaag cacttcgcca gacacaccag acgcctgtca ttatgttgac ggcgcgcggc
    52681 agtgaacttg atcgcgttct cggccttgag ctgggcgcag atgactatct cccgaaaccg
    52741 tttaatgatc gtgagctggt ggcacgtatt cgcgcaatcc tgcgccgttc gcactggagc
    52801 gagcaacagc aaaacaacga caacggttca ccgacactgg aagttgatgc cttagtgctg
    52861 aatccaggcc gtcaggaagc cagcttcgac gggcaaacgc tggagttaac cggtactgag
    52921 tttaccctgc tctatttgct ggcacagcat ctgggtcagg tggtttcccg tgaacattta
    52981 agccaggaag tgttgggcaa acgcctgacg cctttcgacc gcgctattga tatgcacatt
    53041 tccaacctgc gtcgtaaact gccggatcgt aaagatggtc acccgtggtt taaaaccttg
    53101 cgtggtcgcg gctatctgat ggtttctgct tcatgatagg cagcttaacc gcgcgcatct
    53161 tcgccatctt ctggctgacg ctggtgctgg tgttgatgtt ggttttgatg ttacccaagc
    53221 tcgattcacg ccagatgacc gagcttctgg atagcgaaca gcgtcagggg ctgatgattg
    53281 agcagcatgt cgaagcggag ctggcgaacg atccgcccaa cgatttaatg tggtggcggc
    53341 gtctgttccg ggcgattgat aagtgggcac cgccaggaca gcgtttgtta ttggtgacca
    53401 ccgaaggccg cgtgatcggc gctgaacgca gcgaaatgca gatcattcgt aactttattg
    53461 gtcaggccga taacgccgat catccgcaga agaaaaagta tggccgcgtg gaactggtcg
    53521 gtccgttctc cgtgcgtgat ggcgaagata attaccaact ttatctgatt cgtccggcca
    53581 gtagttctca atccgatttc attaacttac tgtttgaccg cccgctatta ctgctgattg
    53641 tcaccatgtt ggtcagtacg ccgctgctgt tgtggttggc ctggagtctg gcaaaaccgg
    53701 cgcgtaagct gaaaaacgct gccgatgaag ttgcccaggg aaacttacgc cagcacccgg
    53761 aactggaagc ggggccacag gaattccttg ccgcaggtgc cagttttaac cagatggtca
    53821 ccgcgctgga gcgcatgatg acctctcagc agcgtctgct ttctgatatc tctcacgagc
    53881 tgcgcacccc gctgacgcgt ctgcaactgg gtacggcgtt actgcgccgt cgtagcggtg
    53941 aaagcaagga actcgagcgt attgaaaccg aagcgcaacg tctggacagc atgatcaacg
    54001 atctgttggt gatgtcacgt aatcagcaaa aaaacgcgct ggttagcgaa accatcaaag
    54061 ccaaccagtt gtggagtgaa gtgctggata acgcggcgtt cgaagccgag caaatgggca
    54121 agtcgttgac agttaacttc ccgcctgggc cgtggccgct gtacggcaat ccgaacgccc
    54181 tggaaagtgc gctggaaaac attgttcgta atgctctgcg ttattcccat acgaagattg
    54241 aagtgggctt tgcggtagat aaagacggta tcaccattac ggtggacgac gatggtcctg
    54301 gcgttagccc ggaagatcgc gaacagattt tccgtccgtt ctatcggacc gatgaagcac
    54361 gcgatcgtga atctggcggt acaggattgg ggctggcgat tgttgaaacc gccattcagc
    54421 agcatcgtgg ctgggtgaag gcagaagaca gcccgctggg cggtttacgg ctggtgattt
    54481 ggttgccgct gtataagcgg agttaaactc cgcatttgta ggcaggataa ggcgtttacg
    54541 ccgcatccgg catttgagca ggatgcctga tgcgacgctg atagcgtctt atcaggccta
    54601 cactccgggg gttattttcc ccacaaccgg cgggaaaaat cttcgatctt gccgctcagt
    54661 cggcgctttt gcatcgtccg cgtccagctt ttcgataacc cggcggcgga gagtaaacgg
    54721 tgatactgat cgtcatcaaa cggcatatgc catgcgatgg cggcagcctc ctgcacggtc
    54781 acatcgctga cacgggaaac caactccagc ggcgcgtccg cagacacttt ccctggcgct
    54841 atcacgctat acaaccaccc caccttacct gtgttttgca tcaactgcgc tatatcgctg
    54901 atatcaaaat ggtaattgag tttatagcaa ggcgagcgcg gctggctgac ctgaattaat
    54961 gcctctcccc agcggaaaat atcgcccata tgaacattac tttccgtgag accgtcggtt
    55021 gagagatttt cgccaaacgc aggcgcaaca aacaactccg cctgttccgg aaattcccgc
    55081 acccagtaga gatagtgctc acgaggataa tgacacagcg ctctgtctgg cccgccgtga
    55141 acctttttct ccgcctgctc gtcaccttcc agccccagct ccgtcagcat caactcacca
    55201 tcaacctgga ttttggcaat tgcgctgggt ttgccttcgg gataagcctg aatcttgcct
    55261 gtgtatacat caaccggata tcgcatctgt tccctctcca cgagaaattt ccccattaga
    55321 ccatatttat tttatgtcag ccgtgctgtt ttcgcggtaa gtatccaggc aggaactcaa
    55381 tagcaaaccg gacgaacgtt agttcatccg gctgtgggga
//
LOCUS       DAEBXN010000013        55369 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.013, whole genome shotgun sequence.
ACCESSION   DAEBXN010000013 DAEBXN010000000
VERSION     DAEBXN010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55369
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.013"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..356
                     /locus_tag="KJL99_000767"
     CDS             <1..356
                     /locus_tag="KJL99_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HBD4435614.1"
                     /translation="YQFHHIRFDCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILKN
                     ELENSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNVS
                     SGQIMSRVKIVVMYN"
     gene            complement(394..1293)
                     /gene="nhaR"
                     /locus_tag="KJL99_000768"
     CDS             complement(394..1293)
                     /gene="nhaR"
                     /locus_tag="KJL99_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HBD4435615.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(1359..2525)
                     /gene="nhaA"
                     /locus_tag="KJL99_000769"
     CDS             complement(1359..2525)
                     /gene="nhaA"
                     /locus_tag="KJL99_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HBD4435616.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLVLKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMVVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            3111..3263
                     /gene="mokC"
                     /locus_tag="KJL99_000770"
     CDS             3111..3263
                     /gene="mokC"
                     /locus_tag="KJL99_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HBD4435617.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(3367..4497)
                     /gene="dnaJ"
                     /locus_tag="KJL99_000771"
     CDS             complement(3367..4497)
                     /gene="dnaJ"
                     /locus_tag="KJL99_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HBD4435618.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(4586..6502)
                     /gene="dnaK"
                     /locus_tag="KJL99_000772"
     CDS             complement(4586..6502)
                     /gene="dnaK"
                     /locus_tag="KJL99_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HBD4435619.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTIPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            6879..7283
                     /locus_tag="KJL99_000773"
     CDS             6879..7283
                     /locus_tag="KJL99_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HBD4435620.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            7309..8022
                     /gene="msyB"
                     /locus_tag="KJL99_000774"
     CDS             7309..8022
                     /gene="msyB"
                     /locus_tag="KJL99_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HBD4435621.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSILM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSTYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            8171..8737
                     /gene="satP"
                     /locus_tag="KJL99_000775"
     CDS             8171..8737
                     /gene="satP"
                     /locus_tag="KJL99_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HBD4435622.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(8772..9359)
                     /gene="mog"
                     /locus_tag="KJL99_000776"
     CDS             complement(8772..9359)
                     /gene="mog"
                     /locus_tag="KJL99_000776"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HBD4435623.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(9474..10427)
                     /gene="tal"
                     /locus_tag="KJL99_000777"
     CDS             complement(9474..10427)
                     /gene="tal"
                     /locus_tag="KJL99_000777"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HBD4435624.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            10706..12136
                     /locus_tag="KJL99_000778"
                     /pseudo
     CDS             10706..12136
                     /locus_tag="KJL99_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
     gene            12206..12982
                     /gene="yaaA"
                     /locus_tag="KJL99_000779"
     CDS             12206..12982
                     /gene="yaaA"
                     /locus_tag="KJL99_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HBD4435625.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(13135..13431)
                     /locus_tag="KJL99_000780"
     CDS             complement(13135..13431)
                     /locus_tag="KJL99_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HBD4435626.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(13645..14931)
                     /gene="thrC"
                     /locus_tag="KJL99_000781"
     CDS             complement(13645..14931)
                     /gene="thrC"
                     /locus_tag="KJL99_000781"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HBD4435627.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIADDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSFGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFSALRKLMMNHQ"
     gene            complement(14932..15864)
                     /gene="thrB"
                     /locus_tag="KJL99_000782"
     CDS             complement(14932..15864)
                     /gene="thrB"
                     /locus_tag="KJL99_000782"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HBD4435628.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVAPVDGALLGDVVTVEAAETF
                     SLNKLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(15866..18328)
                     /gene="thrA"
                     /locus_tag="KJL99_000783"
     CDS             complement(15866..18328)
                     /gene="thrA"
                     /locus_tag="KJL99_000783"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HBD4435629.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKNISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSLSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(18354..18473)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(18409..18474)
                     /gene="thrL"
                     /locus_tag="KJL99_000784"
     CDS             complement(18409..18474)
                     /gene="thrL"
                     /locus_tag="KJL99_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HBD4435630.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(18688..19374)
                     /locus_tag="KJL99_000785"
     CDS             complement(18688..19374)
                     /locus_tag="KJL99_000785"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HBD4435631.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(19774..19914)
                     /locus_tag="KJL99_000786"
     CDS             complement(19774..19914)
                     /locus_tag="KJL99_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4435632.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            20010..20726
                     /gene="arcA"
                     /locus_tag="KJL99_000787"
     CDS             20010..20726
                     /gene="arcA"
                     /locus_tag="KJL99_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HBD4435633.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(20785..22137)
                     /gene="creD"
                     /locus_tag="KJL99_000788"
     CDS             complement(20785..22137)
                     /gene="creD"
                     /locus_tag="KJL99_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HBD4435634.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREASESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(22195..23619)
                     /gene="creC"
                     /locus_tag="KJL99_000789"
     CDS             complement(22195..23619)
                     /gene="creC"
                     /locus_tag="KJL99_000789"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HBD4435635.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAHG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMGGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLDQALGNLLDNAIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(23619..24308)
                     /gene="creB"
                     /locus_tag="KJL99_000790"
     CDS             complement(23619..24308)
                     /gene="creB"
                     /locus_tag="KJL99_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HBD4435636.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDIHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(24321..24794)
                     /gene="creA"
                     /locus_tag="KJL99_000791"
     CDS             complement(24321..24794)
                     /gene="creA"
                     /locus_tag="KJL99_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HBD4435637.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            25005..25874
                     /gene="robA"
                     /locus_tag="KJL99_000792"
     CDS             25005..25874
                     /gene="robA"
                     /locus_tag="KJL99_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HBD4435638.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(25871..26518)
                     /gene="gpmB"
                     /locus_tag="KJL99_000793"
     CDS             complement(25871..26518)
                     /gene="gpmB"
                     /locus_tag="KJL99_000793"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HBD4435639.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            26570..27090
                     /gene="yjjX"
                     /locus_tag="KJL99_000794"
                     /pseudo
     CDS             26570..27090
                     /gene="yjjX"
                     /locus_tag="KJL99_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
     gene            complement(27175..27501)
                     /gene="trpR"
                     /locus_tag="KJL99_000795"
     CDS             complement(27175..27501)
                     /gene="trpR"
                     /locus_tag="KJL99_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HBD4435640.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(27591..29528)
                     /gene="sltY"
                     /locus_tag="KJL99_000796"
     CDS             complement(27591..29528)
                     /gene="sltY"
                     /locus_tag="KJL99_000796"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HBD4435641.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLIQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            29739..31406
                     /gene="ettA"
                     /locus_tag="KJL99_000797"
     CDS             29739..31406
                     /gene="ettA"
                     /locus_tag="KJL99_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HBD4435642.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(31513..31680)
                     /locus_tag="KJL99_000798"
     CDS             complement(31513..31680)
                     /locus_tag="KJL99_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4435643.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVTRHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(31714..32946)
                     /gene="nadR"
                     /locus_tag="KJL99_000799"
     CDS             complement(31714..32946)
                     /gene="nadR"
                     /locus_tag="KJL99_000799"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HBD4435644.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(32967..34349)
                     /gene="radA"
                     /locus_tag="KJL99_000800"
     CDS             complement(32967..34349)
                     /gene="radA"
                     /locus_tag="KJL99_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HBD4435645.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSIMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(34398..35366)
                     /gene="serB"
                     /locus_tag="KJL99_000801"
     CDS             complement(34398..35366)
                     /gene="serB"
                     /locus_tag="KJL99_000801"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HBD4435646.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            35472..36116
                     /locus_tag="KJL99_000802"
     CDS             35472..36116
                     /locus_tag="KJL99_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HBD4435647.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            36144..37159
                     /gene="lplA"
                     /locus_tag="KJL99_000803"
                     /pseudo
     CDS             36144..37159
                     /gene="lplA"
                     /locus_tag="KJL99_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
     gene            37191..37454
                     /locus_tag="KJL99_000804"
     CDS             37191..37454
                     /locus_tag="KJL99_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBD4435648.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(37615..38334)
                     /gene="deoD"
                     /locus_tag="KJL99_000805"
     CDS             complement(37615..38334)
                     /gene="deoD"
                     /locus_tag="KJL99_000805"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBD4435649.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(38391..39614)
                     /gene="deoB"
                     /locus_tag="KJL99_000806"
     CDS             complement(38391..39614)
                     /gene="deoB"
                     /locus_tag="KJL99_000806"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBD4435650.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(39666..40988)
                     /gene="deoA"
                     /locus_tag="KJL99_000807"
     CDS             complement(39666..40988)
                     /gene="deoA"
                     /locus_tag="KJL99_000807"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HBD4435651.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVILAKDENSWQEAAKAVKAAIKLADNAPESTPTVYRRISE"
     gene            complement(41115..41894)
                     /gene="deoC"
                     /locus_tag="KJL99_000808"
     CDS             complement(41115..41894)
                     /gene="deoC"
                     /locus_tag="KJL99_000808"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HBD4435652.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGDEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            42152..43693
                     /locus_tag="KJL99_000809"
     CDS             42152..43693
                     /locus_tag="KJL99_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HBD4435653.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQNEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIANGPV
                     HDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQ
                     IAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAA
                     RYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPY
                     GDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMREFTA
                     NVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISHEQQ
                     MRFSQ"
     gene            43665..44528
                     /locus_tag="KJL99_000810"
     CDS             43665..44528
                     /locus_tag="KJL99_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HBD4435654.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(44742..45524)
                     /locus_tag="KJL99_000811"
     CDS             complement(44742..45524)
                     /locus_tag="KJL99_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBD4435655.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAVLGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(45521..46594)
                     /locus_tag="KJL99_000812"
     CDS             complement(45521..46594)
                     /locus_tag="KJL99_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HBD4435656.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRISEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(46716..46877)
                     /locus_tag="KJL99_000813"
     CDS             complement(46716..46877)
                     /locus_tag="KJL99_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HBD4435657.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(46994..47599)
                     /gene="osmY"
                     /locus_tag="KJL99_000814"
     CDS             complement(46994..47599)
                     /gene="osmY"
                     /locus_tag="KJL99_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HBD4435658.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(47992..49581)
                     /gene="prfC"
                     /locus_tag="KJL99_000815"
     CDS             complement(47992..49581)
                     /gene="prfC"
                     /locus_tag="KJL99_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HBD4435659.1"
                     /translation="MTLSPYLKEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLNGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(49672..50349)
                     /gene="yjjG"
                     /locus_tag="KJL99_000816"
     CDS             complement(49672..50349)
                     /gene="yjjG"
                     /locus_tag="KJL99_000816"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HBD4435660.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRSNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(50364..50810)
                     /gene="rimI"
                     /locus_tag="KJL99_000817"
     CDS             complement(50364..50810)
                     /gene="rimI"
                     /locus_tag="KJL99_000817"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBD4435661.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(50779..51192)
                     /gene="holD"
                     /locus_tag="KJL99_000818"
     CDS             complement(50779..51192)
                     /gene="holD"
                     /locus_tag="KJL99_000818"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HBD4435662.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIASPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            51295..52326
                     /gene="rsmC"
                     /locus_tag="KJL99_000819"
     CDS             51295..52326
                     /gene="rsmC"
                     /locus_tag="KJL99_000819"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HBD4435663.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSCQMGDNARFSLVATANDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGCFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            52603..52689
                     /locus_tag="KJL99_000820"
     tRNA            52603..52689
                     /locus_tag="KJL99_000820"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:52637..52639,aa:Leu,seq:cag)
     gene            52718..52804
                     /locus_tag="KJL99_000821"
     tRNA            52718..52804
                     /locus_tag="KJL99_000821"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:52752..52754,aa:Leu,seq:cag)
     gene            52839..52925
                     /locus_tag="KJL99_000822"
     tRNA            52839..52925
                     /locus_tag="KJL99_000822"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:52873..52875,aa:Leu,seq:cag)
     gene            complement(52964..53200)
                     /locus_tag="KJL99_000823"
     CDS             complement(52964..53200)
                     /locus_tag="KJL99_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HBD4435664.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
     gene            53341..54129
                     /gene="fhuF"
                     /locus_tag="KJL99_000824"
     CDS             53341..54129
                     /gene="fhuF"
                     /locus_tag="KJL99_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HBD4435665.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGYCTLK"
     gene            complement(54167..54844)
                     /gene="bglJ"
                     /locus_tag="KJL99_000825"
     CDS             complement(54167..54844)
                     /gene="bglJ"
                     /locus_tag="KJL99_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710107.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HBD4435666.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIVDDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAEDILLCMRHCEA
                     SNVLHPY"
     gene            complement(54802..>55369)
                     /locus_tag="KJL99_000826"
     CDS             complement(54802..>55369)
                     /locus_tag="KJL99_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBD4435667.1"
                     /translation="LVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQA
                     VELLMCPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILT
                     LSERKVLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQG
                     ARELNLPSVYGDAAEWNTAELRREMSHS"
BASE COUNT        13249 a        14943 c        13816 g        13361 t
ORIGIN      
        1 attatcagtt tcatcatata agatttgact gcatccaggg cgagcaaggt gaattagttt
       61 ttgaacccag tgacgttgaa tattactttg aaccagtgac gatacaggag tcaggaacca
      121 gcatattaaa aaatgagtta gaaaacagtg aggacggtgc gggtcaaata ggctttcaac
      181 tctccaatga tggtacgaat gaaatccagt atggtaaaag caattactac tcttttcagc
      241 atccccatga aggaagtaat cagattcctc tatttattcg cccgcgtaca tatggaaaca
      301 atgtctcaag cgggcaaata atgagccgag tcaaaatcgt ggtaatgtac aactaatcta
      361 tctcccccac cttaactttg ggggagctgc cgattaacgc accgctggac taaaaagcgc
      421 agaataatcc gtattgcaga ttcgctgtac cgccgggtgc tgaatcatcc gctcagcaaa
      481 aatagcatgg tactcttcca tcacattctc gacgcgacca atttctacga cagttttatc
      541 ggcataaaag tcatatgcat aaagcgttgg ggcaacgaag attgcattgt gcatcgcacc
      601 aaaagctttc atcaaagcgg catcatcaaa ctcgccgagg atttctacgt ttaatccctg
      661 ggagttaaac cagttaagca atttgcgccc taacattgaa cgtcgcccag gaatcaaaag
      721 tcgccgttct tccagacaag ccgggaacgg tttttctggt ggtggatttg tacaccagaa
      781 actcacgcca cattcgccaa ttctcacgga gaacaggcct tcctgctgcg tagagtctat
      841 cggacagtca gaaatgatca tatccagttt atgctgactt aattgctcca gcagcatttc
      901 gtgggtggat tcgaagcagc gaagatgaat gggctcgcct tctaccactg cggcgttaag
      961 tacgctactg accaggcgtt tggaaagtgc atcagccacg ccaacgtcaa acaataaatt
     1021 ggattctttg cgatagttca caatatccag catttcctgg cttaaggtga acattttatc
     1081 ggcatagcga tagaccagtt ctcccagctc gctgggttcg agaccacgtc ccttgcgttt
     1141 aaataatttg ccttgcaggc gctcttccag cgctcgaatc tgtccggtaa tggtttgtgg
     1201 agttaaataa agcgcctccg ctgcgccaac cacggaacct tctttataga catgccagaa
     1261 gtaatacaag tggttgtaat tgatatgaga catgctcatt tctctccctg ataacaatga
     1321 aaagggagcc gtttatggct ccccggtaaa ccgtcctgtc aaactgatgg acgcaaacga
     1381 acgcgtaacc agctgtatcc aattaccgcc gaagagatag aaccgaccag gataccgagt
     1441 ttcgcccagt taatcagttc tggatctacg ctaccaaagg ccaggctggc aataaagata
     1501 gacatagtaa aaccgatacc gcacaggatc cccaccacca taatttgctg ataagtcgtt
     1561 ccctcaggca gatgcgccag tttcaaacgc agcgccaacc agcagaacag actaatcccc
     1621 agcggtttgc caatcagcaa gccagcgatg atccccaatg gcagaatgga ggtcaagcca
     1681 tccagcgtga cgccttgcag tgaaacgcca gcattagcaa atgcaaacag cggcaaaatc
     1741 agatacgcca cccacgggtg caacacatgc tccagtcgct tcgctggaga acgcccatgc
     1801 ttctctttca aaggaataaa gaagccgaca attacccccg ccagagttgc gtgaaccccc
     1861 gatttcaaca ccgcagtcca caacaccacg ccaacaagaa tatagacgcc cgtgcggcgt
     1921 acaccacaca gattcaatac cgcgagtacc gcaattgcta cagccgcgac gccaagggag
     1981 gccatcgata agtcattagt gtagaacaat gcgatgataa tgatggcccc aagatcgtcg
     2041 ataatagcca gagccatcaa aaagatcttc agcactaacg gaacacgact tcccaacagc
     2101 gccagtacac caagtgcaaa agcaatgtca gtagccgccg ggatcgccca cccttcgcgg
     2161 gtaatcggat cggcatagtt aaaagccaga tagagtaatg ccggcacaat catcccacca
     2221 atagcggcga taactggaaa tgcggcctgg cgtaagctgg ctagcgatcc ttgcatcagt
     2281 tcacgtttaa cttccagacc gactaacagg aaaaataccg ccatcagcgc atcatttatc
     2341 cataacagca tgtttttgtt gatttcgagt gaaccaaccc ggagctgaac cggcgtctcc
     2401 agaaagtcgt gataccatcc actggttgcg ccgctgttgg ccataatcat cgccaggata
     2461 gcggcaatga taagaataat gcctcccgag gcatcactgc taaagaatcg atgcagatgt
     2521 ttcacttttt atttctcttt caggtgaata gatcgttttt actattttac cccgcacgaa
     2581 tcatcgttaa aatcagatta tagtgtagga ataattcgtt ttttacgagc cattgaagaa
     2641 tttgcgccat cagcggtgag atgcgttttt gacggctgta agcgctcacg agtgacccta
     2701 agcccagata atcatagctt aaggtgagga ttcctgaggg attaattagg aaaagtaata
     2761 attaatttgt tgtcagtcgg cgatgcttgt taggccaata tttcaaaaat aatccagctt
     2821 gtcgtcaata attgcgatga agagataaat taaagttaaa taattaaatc attaaaaaat
     2881 tacgccacaa aaatgcgagg cgtcttcagg attagaatcg ataaaacaaa atgggttaaa
     2941 gagagcgata taatagcggc gggtgcttga ggctgtctgt ctcaggcatt agctgaacgg
     3001 cagatagaga aaagccccga gtgatatttt accatcaacc cgaggcctcc tatatgctga
     3061 acacatgtag agtgcctctt actgaccgta aggtcaagga gaagagagca atgaagcagc
     3121 ataaggcgat gattgtcgcc ctgatcgtca tctgtatcac cgccgtagtg gcggcgctgg
     3181 taacgagaaa agacctctgt gaggttcaca tccgaactgg ccagacggag gttgctgttt
     3241 tcacggctta cgaatccgag taagagcaac ggcggggagt gatccccgcc actttacagg
     3301 tgctcgcata tcttcaacgc accctatttt tacccaggcc tgcccacggg caggcttttg
     3361 gggaggttag cgggtcaggt cgtcaaaaaa cttcttcaca ccatcaaaga agctctttga
     3421 gcgcgggctg ttgtgctcgc cggttgggcc accgaagctt tcttgcagct cttgcagcag
     3481 ctgtttctgc ttttcgttca ggcctaccgg tgtttcgacg acaacgcggc acagcaaatc
     3541 accctgtgcg ccaccgcgga cagacttgac gcctttaccg cgcatacgga atagcttacc
     3601 ggtctgggtt tcgccaggca ctttcagttt gacgcgacca tcaagggtcg gtacttcgat
     3661 ttcgccaccc agcgccgcca tagcgaagtt gatcgggact tcgcaataca ggttgttgcc
     3721 ttcacgctcg aaaatcgggt gctgtttaac ctgaacctga acgtacagat cgcctgccgg
     3781 tgcgccatgc tcgcccgctt caccttcgcc cgcaagacgg atgcggtctc cagtgtccac
     3841 ccctgccggg attttaacgg acagcgtttt gctgcgctca acacgaccat gaccatgaca
     3901 tttgttgcac ggatctttga tcagcgtacc gcggccctga cagtgtggac aggtctgctg
     3961 tacagcgaag aatccctggc gcatctgcac ctgaccagaa ccatgacagg tcggacaggt
     4021 ctgcggctgt gtacctggtt ttgcaccgct accgtggcaa acgtcacact cttccagagt
     4081 cggaatgcgg atctctttgg tcacgccacg tacagcttct tcgagggtga gctccatgtt
     4141 atagcgtaaa tcagcaccgc gcgccgcacg ttgacgacca cgtccgccgc caaaaatatc
     4201 gccgaaaacg tcaccaaaaa tatcgctgaa gtctgcgccg ccgccaaaac caccgccgcc
     4261 catgcctcct tgctcaaacg cagcatgacc atactgatcg tatgccgcac gtttttgcga
     4321 gtcggtcaga acttcataag cttccttgat ctctttaaat ttcgcctcgg cctctttgtc
     4381 accctggtta cggtccgggt ggtatttcat ggccaggcgt ttgtaggcct ttctgatttc
     4441 acgctcttcc gctgttttgg aaacgcctaa aatctcgtaa taatcttgct tagccatctt
     4501 ttttaaattg cccctagatg aatgcacggg cggagaggaa attccccttc gcccgtgtca
     4561 gtataattac ccgtttatag ggcgattatt ttttgtcttt gacttcttca aattcagcgt
     4621 cgacaacatc gtcatctttc gcgttgtttg cagaagcatc agcaccggca gtctgctgct
     4681 gggcatgttg ctgctgggcg atttccatca gtttctggga aacctgtgcc agttcctgca
     4741 ttttcgcttc gatagcggct ttgtcttcac ctttcagagc agtttccagt gcagtcagcg
     4801 cagactcgat agcagttttg tcgtcagccg gcagtttgtc gcctgcttct tcaacctgct
     4861 tacgggtgct gtgcagcaga tggtcgccct ggttgcgagt ctgtaccagc tcttcaaact
     4921 tacggtcagc ttcggcgtta gcttctgcgt cgcgtaccat tttctggatt tcatcttcgt
     4981 tcagaccaga agaagccttg atggtgatct tctgctcttt accgctgttt ttatctttcg
     5041 cggaaacgtg caggataccg tcagcatcga tatcgaaggt aacttcgatc tgcggcatgc
     5101 cgcgcggtgc cgggttgata ccatctaggt tgaactgacc cagagatttg ttatcagccg
     5161 cacgtttacg ttcaccctgc agcacatgga tggttaccgc agactggttg tcttcagcgg
     5221 tagagaacac ctggctgtgc ttggtcggga tagtggtgtt tttcgcgatc agcgtcgtca
     5281 tcacaccgcc catggtttcg atacccagag acagcggggt aacgtccagc agcagtacgt
     5341 cttttacgtc accagtcaga acaccaccct gaacagcagc accgattgct acagcttcgt
     5401 ccgggttaac gtctttacgc ggctctttac caaagaactc agcaactttc ttctgaacca
     5461 ttggcatacg agtctgacca ccaacgagga taacgtcgtc gatatcagat acggacaggc
     5521 cagcatcctg cagtgcaact ttcagcggct caatggaacg gtttaccaga tcttcaacca
     5581 ggctttccag tttcgcacga gtcactttga tgttcatgtg tttcggaccg gtcgcgtctg
     5641 cagtgatgta tggcaggtta acgtcggtct gctgagcgga agacagttcg attttcgctt
     5701 tttctgccgc ttctttcagg cgctgcattg ccagcggatc gttgcgcagg tcaatgccct
     5761 gatctttctt gaattcttca accagatagt tgatcagacg gctgtcgaag tcttcaccac
     5821 ccaggtgggt atcaccgttg gttgccagaa cttcgaaggt tttttcgccg tcaacttcgt
     5881 cgatttcgat aatagaaata tcgaaagtac caccccccag gtcataaacc gcgatagtac
     5941 ggttgccagt gcctttgtcc agaccgtaag ccagcgcagc tgcggtcggt tcgttgatga
     6001 tacgttttac ttccagacca gcgatacggc ctgcgtcttt ggttgcctga cgctgagcat
     6061 cgttaaagta tgccggtacg gtgataacag cttcagttac cggttcaccc aggtaatctt
     6121 cagcggtttt cttcattttt ttcagcactt cagcagaaat ctgcggcggt gccattttct
     6181 ggcctttaac ttcgacccat gcgtcgccgt tatcagcagc aataattttg aacggcatga
     6241 tggaaacatc acgctgtact tcttcgtcct ggaagcggcg gccaatcagg cgtttaatcg
     6301 caaacagggt gttttgcggg ttcgtcactg cctgacgttt agccggctga ccaaccagag
     6361 tttcaccatc ctgggtatag gcaatgatag aaggcgtggt gcgatcgcct tcggcgttct
     6421 ccagcacgcg aggaatggtg ccatccataa tcgctacaca agagttggta gtacccaggt
     6481 cgataccaat tattttaccc atctaaacgt ctccactata tattcggtca tcatgtggtt
     6541 gtgagtctgt aataggggcg aaacgtctgg tttcaactgc ccaatttcat ttttttgcag
     6601 actcactcac tgcggttgac tactaaatgg ggtcgtaacc cacgtcatca agggggagat
     6661 gcaaaaattt tttgtgcttt tgcgcagaaa tttcgcgata tggcaggaca agacgtaaaa
     6721 ttgacagcaa ttttattcat ataaagaatg aatcgcagcc aaggcgttca tcaaaaaatt
     6781 gtaataaaaa gaaaagatta cgtgcctgaa tcttctcttt atcagcagta aactagtggg
     6841 tattcatccc cctacctctt cccactaaga gaatccttat gaaatccgtt tttacgattt
     6901 ccgccagcct ggcgattagc ctgatgctgt gctgcacggc gcaggcaaac gaccataaac
     6961 tcctcggcgt cattgcaatg ccgcgtaacg aaaccaacga tctggcgctg aaacttcctg
     7021 tttgtcgcat tgtgaaacgc atacaactct ctgccgacca tggcgattta cagttaagtg
     7081 gtgcatcggt ttatttcaaa gccgcccgta gcgccagtca gagcctgaat attccttcag
     7141 aaataaaaga agggcaaacc actgactgga tcaacattaa cagcgataac gacaataaac
     7201 gctgcgtctc aaaaatcacc ttttccggtc atacggtgaa ctcatcggat atggccacgc
     7261 tgaaaattat cggcgacgac taacgccaga atatagaagc cacaaaaaat gaatgttaat
     7321 tacctgaatg attcagatct ggattttctt cagcattgta gtgaggaaca gttggcaaat
     7381 ttcgcccgat tgctcaccca taatgaaaaa ggcaaaactc gcctctccag catactgatg
     7441 cgcaacgaac tgtttaaatc gatggaaggg catcccgagc aacatcgccg caactggcag
     7501 ctgattgccg gagaattaca gcattttggt ggcgatagta tcgccaacaa actgcgcgga
     7561 cacggtaaat tgtatcgggc cattttgctc gatgtttcaa agcgattgaa gctgaaagcc
     7621 gacaaagaga tgtctacgtt tgaaattgag caacagttac tggaacaatt tctgcgtaat
     7681 acctggaaga aaatggacga ggaacataag caggagtttc tgcacgcggt cgatgccagg
     7741 gtgaatgagc tggaagagct gctgccgctg ctgatgaaag acaaattatt ggcaaaaggt
     7801 gtgtcgcatt tgctttccag ccaactgacc cgcattttac gcacccacgc agcaatgagc
     7861 gtacttgggc atggtttgct gcgcggcgcg gggctgggag gccctgtggg cgcagcatta
     7921 aatggggtta aagcggtcag cggcagcacc tatcgcgtga cgattccagc cgtactgcaa
     7981 atcgcctgcc tgcgccggat ggttagcgcc actcaggtct gataccggaa accggggaaa
     8041 attttcattt tcccctcaaa agatcgtaga cactgcccca ctggctgatt attatgccgc
     8101 gccctgaaaa cactacagtt attcagggaa attatttcac cattcattcg atgatgattt
     8161 ttgaggaatt atgggcaaca ctaagttggc taatccggca ccgctgggcc tgatgggctt
     8221 cggcatgacc accattctgc ttaacctgca caacgtgggt tatttcgctc tggacggtat
     8281 tattcttgcc atgggcattt tctacggcgg catcgcgcaa atttttgctg gtctgctgga
     8341 gtacaaaaaa ggcaacactt tcggtttaac cgcattcacc tcttacggtt ctttctggct
     8401 gacgctggtt gcgattctgc tgatgccgaa actgggtctg accgatgcgc caaatgcaca
     8461 gttccttggt gtctacctgg gtctgtgggg cgtatttacg ctgtttatgt tcttcggcac
     8521 tctgaaaggc gcgcgcgtcc tgcaattcgt tttctttagc ctgaccgtgc tgtttgccct
     8581 gctggcgatc ggtaacattg ccggtaatgc cgcgatcatc cactttgccg gctggattgg
     8641 tctgatctgc ggtgccagcg caatctatct ggcgatgggt gaagtgctga acgagcagtt
     8701 tggtcgcacc gttctgccga ttggtgaatc ccactaattg tcttgagatc cccccgctcg
     8761 gggggatttt tttattcgct aacgtcgcgt cttgcactct tcggtctgaa tgcagcaacc
     8821 acttctggtg ccgtttcaac gtatggccct tccagcaact gaatgcagta cggtacgctg
     8881 gcaaaaatac catgcaccac aacgttaccc tcagcgtcct tcacaccttc cagcgtctct
     8941 ttaatagact tcggctgacc gggtaagtta aggatcagcg cctgtttgcg aatcacgccc
     9001 acctgacgcg aaaggatcgc agttggtaca aaatgcaggc tgatctggcg catctgttca
     9061 ccaaagccag gcatctcgcg gtccgctact gccagcgtcg catcgggcgt tacgtcacga
     9121 cgcgccgggc cagttccgcc cgtggtgagc accagatggc aactcatttc atccaccagc
     9181 tcacacaacg tttgctcgat gatcgcctgc tcatcgggga ttaagcgggt ttccagttca
     9241 aacggcgtgg ttagcgccga tgtcagccat tcttccagcg ctggaatgcc tttatcctga
     9301 taaacgccgc tggatgcgcg atcagagatg gaaactaagc caatacgtaa agtattcatc
     9361 aagatttttc cgtttaaaca atctcgttaa acagaatgat acactgcgaa gggagtgaca
     9421 gacaggcttc agaagaggta gcgtgaccga cttcccggtc acgctaagaa tgattacagc
     9481 agatcgccga tcattttttc cagtttttcc tggtcaatag caaacttacg gataccttcc
     9541 gccagtttat ctactgccat tggatcctgg ttgtgctgcc acaggaactc ggactcagtg
     9601 atacgcgccg gacgcgcttt cacttcgccg gtgtaagaca gtttacgttc gatagcccct
     9661 tcgctctccg ccagctcttt cagcagtgcc ggtgcgatgg tcagacggtc acagcctgcc
     9721 agttccagaa tttcgccgat gttacggaag cttgcgccca taaccacggt ttcataaccg
     9781 tgctctttgt agtactggta gatttcagat acagaaacca cgcccggatc ttctgccgga
     9841 gcgtactctt tcttatcggt attcgctttg taccagtcaa gaatacggcc aacaaacggc
     9901 gagatcagga acacgcccgc ctccgcacaa gcacgagcct gagcgaagga gaacagcagg
     9961 gtcaggttac agttgatacc ttctttttcc agctgttctg cagcacggat accctgccag
    10021 gtagaagcca gtttgatcag aatacgatcg ttgctaatac ctgcatcgtt gtagagtttg
    10081 atcaggcgtt ttgctttcgc aattgacgct tcagtgtcat aggaaagacg cgcatcaact
    10141 tcagttgaga tacggcccgg aaccagtttc aggatttcca gaccaatatt tactgccagt
    10201 ttgtcggtcg cgtccacgat ctgctgcgcg cgatcgttgc tctgctgttt cgcccaggcg
    10261 acagcatcat caatcaactt acggtattcc ggaatctgcg ctgcgttaag aatgagagaa
    10321 gggttggttg tggcatcctg cggttgatac agcttcattg ccgcgatgtc cccagtgtcg
    10381 gccactacgg tggtgtactg acgaagggag gtcaatttgt ccgtcatgat agtatttctc
    10441 tttaaacagc ttgttagggg gatgtaaccg gtctgccctg atgatatcac gacgcaacgt
    10501 cagcgcaacc gccgacaaga cggttatcgc caggcgagac tgtttcggat ttctgacact
    10561 gcgttgatat cgcccaccat ttttatacaa aacctcatgt atgctacgca gaagttatca
    10621 agtacctcgt agcgtatata cttcttaaac aattggtaac gtttacacag gaaagtcatc
    10681 gcgaccggca ataagaggga tatgcatgcc agattttttc tcctttatta acagcgtcct
    10741 ttggggatcg gtaatgattt acctgctctt cggcgcaggt tgttggttca cttttcgcac
    10801 cggatttgtg cagtttcgct acatccgcca gtttggcaaa agtcttaaaa atagcattca
    10861 tccacagcca ggcggtttaa cctcatttca gtcattgtgt accagtcttg cggcgcgcgt
    10921 gggtagcggc aacctggccg gcgttgcgct ggcaattacc gccggtggac caggtgccgt
    10981 cttttggatg tgggttgctg cgtttatcgg catggcgacc tcgtttgccg aatgttccct
    11041 tgcacaactt tataaagaac gtgacgtcaa tgggcagttt cgtggcggac cggcatggta
    11101 tatggcgcgc gggctgggga tgcgctggat gggcgttctg ttcgccgtct ttttgctcat
    11161 cgcctacggc ataattttca gcggagttca ggcgaacgcc gttgcccgag ccctgagttt
    11221 ttcttttgat tttcctccgc tggtgacggg cattattctc gctgtctttg ctctgctggc
    11281 aatcactcgt ggtcttcatg gcgtcgcccg gctcatgcag gggtttgtcc cgttgatggc
    11341 gataatctgg gtactgacca gcctggtgat ttgcgtaata aatatcgggc aacttcccca
    11401 cgtcatttgg tctatttttg agagtgcttt tggctggcag gaagcggcag gcggcgcggc
    11461 gggatatacc ttaagccagg cgattactaa cggttttcag cgcagtatgt tttccaatga
    11521 ggtgggaatg ggatcgacgc caaacgcggc agcggcagct gcatcctggc ctccgcatcc
    11581 ggcagcacaa gggattgtcc agatgattgg catttttatc gacaccctgg tcatctgtac
    11641 ggcaagcgcc atgctgatat tactggcggg taacggcaca acctacatgc cgctggaagg
    11701 tattcagctt atccagaagg cgatgcgggt gctaatgggt tcctagggtg ctgaatttgt
    11761 taccctcgtg gttattctgt ttgccttcag ctccatcgtt gccaactaca tttatgccga
    11821 aaacaatctc ttctttttac gcctgaacaa ccctaaagcg atctggtgtt tgcggatctg
    11881 caccttcgca acggtcatcg gcggcacctt gctaagcctt ccgctgatgt ggcaactggc
    11941 agatatcata atggcctgca tggctattac caatttgacc gccattttac tgctctcgcc
    12001 tgtggttcat accattgcca gtgattatct acgccagcgt aaactcggcg tgcgcccggt
    12061 gtttgatccg ttgcgttatc cggatatcgg tcgccagctt tctccggacg cgtgggatga
    12121 tgtttcgcag gagtaatcac aactatcgat caactcattc tctttttttg ctaaagtcgg
    12181 cataaatttc ctgcaaggac tggatatgct gattcttatt tcacctgcga aaacgcttga
    12241 ttaccaaagc ccgttgacca ccacgcgcta tacgctgccg gagctgttag acaattccca
    12301 gcagttgatc catgaagcgc ggaaactgac gcctccgcag attagcacgc tgatgcgcat
    12361 cagcgacaaa ctggcgggta tcaacgccgc tcgctttcat gactggcagc cagatttcac
    12421 gccggcgaat gcccgccagg cgattctggc gtttaaaggt gatgtctaca ccggcttgca
    12481 ggccgaaacc ttcagcgaag acgatttcga ttttgcccaa cagcatttgc gaatgctttc
    12541 cggcttgtat ggcgtactgc gcccgctcga tttaatgcag ccttatcgtc tggaaatggg
    12601 gatccgtctt gagaatgccc gagggaaaga tctgtatcaa ttctggggag atatcatcac
    12661 caacaagctg aacgaggcgc tcgcagcaca aggcgataat gtggtgatta atctggcgtc
    12721 agatgagtat tttaaatcgg tgaagccgaa gaaattgaat gccgagatta tcaagccggt
    12781 gttcctcgat gagaagaacg gcaaatttaa gatcatcagc ttctacgcta agaaagcacg
    12841 cggtctgatg agtcgtttca ttattgaaaa tcggctgacc aaaccagagc aactgactgg
    12901 ttttaatagc gaaggttact tctttgatga agattcctcc agcaatggcg aactggtgtt
    12961 taaacgctac gagcagcgtt aagtttgtag tcaatgccgg atgcggcgtg aacgccttat
    13021 ccggcctaca aaagcatgca gattcaatat attgcagaat taacgtaggc ctgataagac
    13081 gcgccagcgt cgcatcaggc gctgaatgcc ggattgttac ccggcatttg tcatttagcg
    13141 gtgatgtttg cctggaccat gaccgccgtg atgatcatga ggagctttct tatggtggcg
    13201 cggcggtggc ggcggtccgt gtgggtgcca gcgattgcct cgccattcat aatgttgttt
    13261 ccaccagccg tggtcgcgcc agtgacctcc atcccagtaa tagccacgat tatcacgatc
    13321 gcctatctgt aattttactg acgggactaa cgtaatttcc gcagcctgtg ctgccatggg
    13381 agcgaccaga accagggaaa gtgcgagtac gatagattgc atctttttca caggttactc
    13441 cttttatcat tgcccgttat gggtcgatat ccggagatta cgcgatgaga acgacggttg
    13501 ttattctccg caatcctaat ctctaagtta ccgcatttat tgataatttc tcctttttcc
    13561 ctgtcgtttt tctccataat ttcttcataa aaaagccggg ctgcataaaa gcaaacccgg
    13621 cctgattgag ataatgaata gattttactg atgattcatc atcaatttac gcaacgcaga
    13681 aaaatcggcg ggcagattat gtgaaagcaa gggtaaatca gcacgttctg ccagctcttt
    13741 tggcagatcc aacgtttcac cgagaatcgc ttccacgctc tctttaaatt tcgccggatg
    13801 cgcggtgccg aggaacaagc catattcgcc tggattcaac tggtcacgca gcgcacgata
    13861 cgctacggca gcgtgcggct ccgaggtgta gcccagttct tttaactcac gcattgtctg
    13921 ttgcgtggtt tcatcatcca cggctgcata acccagctct ttcagttgcc agattttgcg
    13981 gcggaacaac tcttccacac gcggccagtt gttcggctga ctcacgtcca tcgcgttgga
    14041 taacgtcgcc tgagtcgctt tgggtgacca ctgaccgtcg tgcaggaaac gtggcacggt
    14101 atcgttcacg ttggtcgcag caataaaacg tttcaccggc agaccgaatg acttcgccag
    14161 cagacccgcc gtcaaatcgc cgaagtttcc gcttggcacc gagacaacca gctggttgcg
    14221 cgcctcctgc ggcagctgcg caacagcttc aaagtagtag caaatctgcg ccagcaaacg
    14281 gctgatgtta atcgagttag ccgagtttaa ccccagcgcc actttcagtt cttcatcatc
    14341 aaacgcctgc ttcaccagcg cctgacaggc atcgaaatcg ccgtcgatgg caacagtttc
    14401 gatattgccg cccaacgtac agaacagttt ttcttgcagt ggactgattt tgcctcgtgg
    14461 atagaggata accactttca cattcggcaa accgtagaaa gcatgagcca ctgcagctcc
    14521 ggtatcaccg gaggtcgcgg tcagaatggt cactggctta tcatccgcaa tatgggtcag
    14581 catttgtgcc ataaagcgac cgccgaaatc tttaaatgcc agcgttggcc cgtggaacaa
    14641 ttccagacaa ccgacatcgc tttcaacatt ggcgaccgga gccgggaagg caaacgccgc
    14701 gcgcacgcgc tcttccagga tttcctgtgg gatttcatca ccaataaacg ccgagaggat
    14761 cttcgcactg cgggtgacaa aatccagctt cagcatctca tcaatttcag tcaggctgaa
    14821 ttccggcagg tcgtgcggaa aaaacagccc ctgatttttg cccaacccct gggttacggc
    14881 ttgcgcaaag ctgacctgct cgttgtgatc tttcagattg tagagtttca tttagttttc
    14941 cagtactcgt gcgcccgccg tatccagccg gcaaatatga acaaaacctt cctgattttg
    15001 caggtagttc ttacccaacc agtcggcaac gcgctgggcg gtatccggct tgtcacatag
    15061 agcgaacaag gtcgggccgg agccggagat accgctcgct accgcgccga tttccgcgac
    15121 cgcctgccgc gcctgccgga agccaggcag taaccgttca cggtagggtt cagcgataac
    15181 atctttcatc agcttcgcgg caagctcagg ctgacgggaa tagcaggcgt gaatgaagcc
    15241 agccagatgt cgcccgtgcg caatgcaatc ctggcggcga tactgcgccg gtaaaatagc
    15301 ccgggcttct gccgtcgaga ctttaattcc cggatacgcc agcacccaca gccactcatc
    15361 aaaccctggc acttgctggc tgatgatgtc gttttcttcg atcatcaact gcataccacc
    15421 aagaaaacac ggtgccacgt tgtcgtaatg aatgctgccg gagatacgtc cttccagctc
    15481 gcccatcaaa gccagcaaac gagtgtcatt aagcggcttg ccgcaatgtt cattcatcgc
    15541 catcagcgcc gcgaccaccg aacaggcgct ggagcctaag cccgaaccga tcggcatatt
    15601 cttttccaga gtcatcgcca ctggaatttg cttgccaagc tcctggcaaa aacgctccca
    15661 gcactgataa acgatatttt cccgtggttc tgacggcagc ttatcggcaa agcgtccgag
    15721 tttgttgaga ctgaatgtct ctgccgcctc aaccgtgact acatctccga gcaatgcacc
    15781 atcaacgggt gccaccgccg ccccgagcac atcaaacccg acgctcatat tggcactgga
    15841 agccggggca taaactttaa ccatgtcaga ctcctaactt ccatgagagg gtacgtagca
    15901 gatcggcaaa gacaccggca gcggtaacgt cattgcccgc accatatccg cgcagcacca
    15961 acggcagcgg ctgataatag tggctataaa aggccagggc gttttcgcca tttttcactt
    16021 tgaacagcgg atcattccca tccacttcgg caatcttcac gcggcagaca ccatcttcat
    16081 caatattgcc aacatagcgc aaaacttttc cttcatcacg ggccttcgcc acgcgcgcgg
    16141 caaagagatc gtcgagctgt gacagattcg ccataaaagc ggcaacatca ccctcggcgt
    16201 taaactctgc gggcagcaca ggttcaattt caatatccgc cagctccagt tcacgtcccg
    16261 tttcacgagc gagaatcaat agtttacgcg ccacatccat accagaaaga tcatctcgcg
    16321 ggtccggttc ggtataaccc atttcccgcg ccagcgtggt cgcctcggag aggctcatgc
    16381 cttcgtctaa cttgccgaag atataagaaa gcgaacctga aagaatgccg gagaacttca
    16441 ccaattcatc acctgcattg agcagatttt gcaggttctc aataaccggt aatccagccc
    16501 caacgttggt gtcatagagg aatttacgcc gcgatttttc cgccgcataa cgcaactgat
    16561 gatagtaatc catcgacgag gtgttggcct ttttgttcgg cgtgacaacg tggaaacctt
    16621 cgcgcaggaa gtcggcatat tgatccgcca ctgcctggct ggaggtgcag tcaacaatga
    16681 ccgggttcag cagatgatat tctttcacga ggcgaattaa gcgcccgaga ttaaacggct
    16741 ctttggcttg tgcaagttct tcctgccagt tttccagatt aaggccatgc acactagtga
    16801 gcagagcctt cgagttggca acgccgcaga cacgtaagtc gatatgttta ttcttcagcc
    16861 agctttgctg acgcttcagt tgctccagca gcgcaccgcc aacgccaccg acgccaatca
    16921 caaacacttc gataacctga tcggtattga acagcatctg atgagtaacg cgcacgccag
    16981 tggtcgcatc atcgttattt accacgacag agattgagcg ttcagaagat ccctgagcaa
    17041 tggcgacaat gttgatattg gcgcgggcca gcgcggcaaa gaatttcgcc gagatcccac
    17101 gcaaggtgcg cataccatca cctaccaccg agataatggc cagccgttcc gtcaccgcca
    17161 gcggctccag taagccttct ttcagttcca ggtagaactc ttcctgcatt gcccgttcag
    17221 ctcgcacaca gtcgctttgt ggaacgcaga aactgatact gtattcggaa gatgattgcg
    17281 taatcagcac cacggaaata cgggcgcgtg acattgcagc aaagacgcgc gccgccatgc
    17341 caaccatccc tttcatcccc gggccggaaa cgctgaacat tgccatgtta ttcagattgg
    17401 aaatgccctt gaccggtaat tcgtcttcat cacggctggc accaatgagc gtacctggtg
    17461 cttgaggatt tccggtattt ttaatcaggc aagggatctg gaactgggcg atgggggtaa
    17521 tggtgcgggg gtgaagaact ttagcgccga agtaggaaag ctccatcgct tcctggtagg
    17581 acatcgactt caacaacctc gcatcgggca cctgacgcgg gtcgcaggta tagaccccgt
    17641 caacgtccgt ccaaatctcg caacaatcgg cgcgtaaaca ggcagccagc accgccgcgg
    17701 agtagtcgga accgttacgt ccaagcacca ccagttcgcc tttttcatta ccggcggtga
    17761 aacctgccat caacaccatg tgatcagccg gaatgcggct tgccgcaata cggcgggtgg
    17821 actcagcaat atcgacggta gattcgaggt aatgccccac tgccagcagt ttttcgaccg
    17881 gatcgataac ggtaacgttg tgaccacgcg cttctaacac gccggccata atggcgatcg
    17941 acattttctc gccacggcaa atcagcgcag cgttgatgct atccgggcac tgccccaaca
    18001 aactaatgcc atgcaggaca tgttttattt gggcaaattc ctgatcgacg aaagttttca
    18061 attgcgccag cgggaacccc ggctgggcgg cggcgagtcc cgtcaaaagt tcggcaaaaa
    18121 tacgttcggc atcgctgata ttgggtaaag catcctggcc gctaatgttt ttttcaatca
    18181 tcgccaccag gtggttggtg attttggcgg gggcagagag gacggtggcc acctgcccct
    18241 gcctggcatt gctttccaga atatcggcaa cacgcagaaa acgttctgca tttgccactg
    18301 atgtaccgcc gaacttcaac actcgcatgg ttgttacctc gttacctttg gtcgaaaaaa
    18361 aagcccgcac tgtcaggtgc gggctttttt ctgtgtttcc tgtacgcgtc agcccgcacc
    18421 gttacctgtg gtaatggtga tggtggtggt aatggtggtg ctaatgcgtt tcatggatgt
    18481 tgtgtactct gtaattttta tctgtctgtg cgctatgcct atattggtta aagtatttag
    18541 tgacctaagt caataaaatt ttaatttact cacggcaggt aaccagttca gaagctgcta
    18601 tcagacactc tttttttaat ccacacagag acatattgcc cgttgcagtc agaatgaaaa
    18661 gctgaaaaat acttactaag gcgtttttta tttggtgata tttttttcaa tatcatgcag
    18721 caaacggtgc aacattgccg tgtctcgttg ctctaaaagc cccaggcgtt gctgtaacca
    18781 gtcgaccagt tttatgtcat ctgccactgc cagagtcgtc agcaatgcca tgactcgttc
    18841 gcgtaaagct tgcagttgat gttggtctgc cgttgtgtca cttttcgccg gttgttgtat
    18901 taatgttgct aattgatagc aatagaccat caccgcctgc cccagattga gcgaaggata
    18961 atccgccacc atcggcacac cagtaagcac gtcagccaac gctaactctt cgttagtcag
    19021 cccggaatct tcgcgaccaa acaccagcgc ggcatggctc atccatgaag atttttcctc
    19081 taacagcggc accagttcta ctggcgtggc gtagtaatga tatttcgccc gactacgcgc
    19141 agtggtggcg acagtgaaat cgacatcgtg taacgattca gccagtgtcg ggaaaacttt
    19201 aatattatca ataatatcac cagatccatg tgcgacccag cgggtggctg gctccaggtg
    19261 tgcctgacta tcgacaatcc gcagttcgct aaaacccatc gttttcattg cccgcgccgc
    19321 tgccccaata ttttctgctc tggcgggtgc gaccaggata atcgttatac gcatattgcc
    19381 actcttcttg accaaataac cgcgaacagg gtgatcactg tcaacttatt acgcggtgcg
    19441 aatttacaaa ttcttaacgt aagccgcaga aaaagccctt tacttagctt aaaaaaggct
    19501 aaactatttt ctgactgcac taacggttta attgttaaaa aatgctacat atccttctgt
    19561 ttacttagga taattttata aaaaataaat ctcgacaatt ggattcacca cgtttattag
    19621 ttgtatgatg caactagttg gattattaaa ataatgtgac gaaagctagc atttagatac
    19681 gatgatttca tcaaactgtt aacgtgctac aattgaactt gatatatgtc aacgaagcgt
    19741 agttttattg ggtgtccggc ccctcttagc ttgttatgtt gctgttaaaa tggttaggat
    19801 gacagccgtt tttgacactg tcgggtcctg agggaaagta cccacgacca agctaatgat
    19861 gttgttgacg ttgatggaaa gtgcatcaag aacgcaatta cgtactttag tcatgttacg
    19921 ccgatcatgt taatttgcag catgcatcag gcaggtcagg gacttttgta cttcctgttt
    19981 cgatttagtt ggcaatttag gtagcaaaca tgcagacccc gcacattctt atcgttgaag
    20041 acgagttggt aacacgcaac acgttgaaaa gtattttcga agcggaaggc tatgatgttt
    20101 tcgaagcgac agatggcgcg gaaatgcatc agatcctctc tgaatatgac atcaacctgg
    20161 tgatcatgga tatcaatctg ccgggtaaga acggtcttct gttagcgcgt gaactgcgcg
    20221 agcaggcgaa tgttgcgttg atgttcctga ctggccgtga caacgaagtc gataaaattc
    20281 tcggcctcga aatcggtgca gatgactaca tcaccaaacc gttcaacccg cgtgaactga
    20341 cgattcgtgc acgcaacctg ctgtcccgta ccatgaatct gggtactgtc agcgaagaac
    20401 gtcgtagcgt tgaaagctac aagttcaatg gttgggaact ggacatcaac agccgttcgt
    20461 tgatcggccc tgatggcgag cagtacaagc tgccgcgcag cgagttccgc gccatgcttc
    20521 acttctgtga aaacccaggc aaaattcagt ctcgtgctga actgctgaag aaaatgaccg
    20581 gccgtgagct gaaaccgcac gaccgtactg tagacgtgac gatccgccgt attcgtaaac
    20641 atttcgaatc tacgccggat acgccggaaa tcatcgccac cattcacggt gaaggttatc
    20701 gcttctgcgg tgatctggaa gattaatcgg ctttaccacc gtcaaaaaaa cggcgctttt
    20761 tagcgccgtt tttatttttc aaccttattt ccagatacgt aattcatcgt ccgttgtaac
    20821 ttctttactg gctttcattt tcggcagtga aaacgcatac cagtctatat tacgagtcac
    20881 aaacatcatg ccggccagcg ccaccaccag cacactggtt cccaacaaca gcgcgctatc
    20941 ggcagagttg agcagtcccc acatcactcc atccagcaac aacagcgcga gggtaaacaa
    21001 catgctattg cgccagcctt tcaataccgc ctgcaaataa ataccgttca ttaacgcccc
    21061 aatcagactg gcgattatcc atgccacggt aaaaccgata tgttcagaaa gcgccagcaa
    21121 gagcaaataa aacatcacca atgaaagccc caccagcaaa tattgcattg ggtgtaaacg
    21181 ttgcgcggtg agcgtttcaa aaacaaagaa cgccataaaa gtcagtgcaa tcagcagaat
    21241 ggcgtactta gtcgcccggt ccgttaattg gtattgatcg gctggcgtcg tcactgcgac
    21301 gctaaacgcg gggaagtttt cccagccggt atcattgcct gaagcaaaac gctcaccgag
    21361 attattagca aaccagctgc tttgccattg cgcctgaaaa cctgactcgc tagcttcccg
    21421 tttggctggt agaaaatcac ctaaaaaact gggatgcggc cagttgctgg ttaaggtcat
    21481 ttcgctatta cgcccggcag gcaccacaga aagatcgccg gtaccgctta aattcagtgc
    21541 catattcagc tgcaggttct gcttccgcca gtccccttcg ggtaaaggga tatgcacgcc
    21601 ctgcccgcct tgctctaacc cggtaccggg ttcaatggtc agtgccgttc cgttaacttc
    21661 aggcgctttc accacaccaa taccacgcgc atccccgacg ctaatcacaa taaagggctt
    21721 gcctaagatg atatttggcg cgttgagctc gctaagacgc gaaacatcga aatcggcttt
    21781 tagcgttaaa tcactgtgcc agacctgacc ggtataaatc cctatcttgc gttcttccac
    21841 gttctgattg ccatcaacca tcaatgactc aggtaaccaa aaatggataa aacttcgttt
    21901 ccgctccacg gttttatcct cttcctgcac cgtataaagc tcggtcacag gaatagcgat
    21961 aagcggccca acgagttttt gcggcccgct ggtactttgg cgaatcgcat cttccacatc
    22021 gctacggtaa tcggcacgtt cgacaatcac ttgccgaatc agcattatcg gaatcaacaa
    22081 cagcaatact gcaccaaaca aggtggtcat tttccagaac aggggggatt tcaacatagt
    22141 cttctccttt gcaatggcag caggatacga aggctatgtg ggaagaattt gaagctatgt
    22201 gaagtgacgg tgaagtcgta gcgaggccag cacgccacct tcctgcacgt tgcgcagcgt
    22261 gacttcgccg ttaaacaaac gggcgacctc actgacgaac gccaacccca gaccgctgct
    22321 tttttgccca tttgcacgcg gcaaagagta aaagcgttca aaaatacgtg acagcgcgta
    22381 gtcaggaata ccactaccgg tatccagcac cttaagcgtg acgtgttcct gatccacttc
    22441 ggcgcttagc gttatgcaac cgctctcggg agtaaaatcg atggcgttat ccagtaaatt
    22501 ccccagcgcc tgatccagta acgccggttc agcagcaaca ttaacctcgg tgggcataac
    22561 atgcaaagtg atgttttttt ctgccaactg cacggtgcgc gcttcgctga cgcggcgaaa
    22621 taatgccgcc acatcaacag cagtcagaac gacttcctga cgattctcca gtcttgcctg
    22681 gcgtagtaac gtttccacca gcgcctgcat tcgcgcattt tgtgtcagaa tgttgtcagt
    22741 aaaacgagcc actacttcag gcggcggacc ttcgcgtaaa atttccgccg cgccacgaat
    22801 cgccgccagt gggcttttta gctcatgggt taacgcataa acatactgct caatatagtt
    22861 tttcccttcc agcttcacgc gcatactttc cagcgcctgc gcgagtttac gcagctcgct
    22921 actaccgaga tcgggaagag gaacgggctt attgtcagtg acggaatcag catagcgagt
    22981 gagccgggca atggagcggt tgatccacca aaccatgcct gcgccaatca ccagtgcaat
    23041 ccccaacaaa atggcgctgg cccataaaat tcgccgctcg ctgcgtttaa tgaccggagc
    23101 catcgccgcg ttcggtttgc ctacgctcaa aacgccaata agccgcgagc cgcccataat
    23161 cggcgcggca acatacatca ccgaactttc gggatcggca ggattttgca acgtgctgcg
    23221 cgcaccatac tgaccacgca acgttagcca gacgtcattc cagcgcgaat aatcctgtcc
    23281 aacggcttta tttgccgaat cgaacaatac tttgccatgc gcatcggtca tatagacatg
    23341 atattcattg cgcactttgt taatgccacc aatattggcg cgaaacgggc gatgttgtag
    23401 ctgattaaac gcctgcgcca gttgcccatg cgttgggtcc ccagagagca aatcgggacg
    23461 cgccagctcc gcaagtaacg ttgccgtgtc gatcaacgtc ccctccgttg ctcttcgcac
    23521 gcccggttta acttctttga caaaaatagc cagtacgaac caggccgcca ccgccaccag
    23581 taaaaaatag cccagcagca accgcatgcc gatacgcatt acaagcccct caggctatat
    23641 cccatgccgc gatgagtatt aatcggtgaa agatcggggt tgatggcgcg cagcttggca
    23701 cgcagcgttt taatgtggat atcgacggtg cgatcgtagg tgtcctgcgc atcttcccac
    23761 acgctatcca ttagttgctg gcgggaccag acgcggcctg gcgacttgag taacgtcttc
    23821 agcaataaaa actcataccg cgtcagcgtt aatggcgtgt caaaccagct gatctgcgcc
    23881 gcgggttcat tcaattcaaa atgaccaata cggatgacgg gagacggcgt cgagaacttc
    23941 ttcacccgac gcagtaaggt gcgcaccctg gcgcacactt cgcggggtga aaacggttta
    24001 gccacatagt cgtcagcgcc aatttccagc ccaagcaggc gatcgacctc ttcacttcgg
    24061 gccgtcagga acagtacagg taacgccgga tggagcgcca gtaactggcg gcacaattca
    24121 aagccactga tatcaggcaa accaacgtcg agaatcatga cgtcgggaac ctgctggcga
    24181 gctttatcca gcaccggcaa acctcgctca aagacctcga cggcaaaacc ttcctgctgc
    24241 aacatgtaga ccagcgtgtc ggctatccct tgctcatctt ccactaacca gaccgtttcc
    24301 cgttgcataa atcgcctctg ttattgccgc cacggcatga caggaaccgc gctaatcgcg
    24361 tttttcggcg aaccttctac aactttgtcg gagtaagcca gataagcgag cgcgttgcgt
    24421 ttggcgtcat aaaagcgcac gacctgtaac gacttaaaga ccagggacgt gcgttttttg
    24481 aatactacct cgccctgagc tttgccgttt ttaatacgat ccgacagttc aatcggcccg
    24541 acttgctgac aagaaatggc cgcatcggag gtatcttctg ccaggcccaa tcccccttta
    24601 ataccaccgg ttttcgcccg gctcacataa caggtgacat ttttcacatc gggatcgtca
    24661 aaggcttcca caacaatttt gtgatccggg ccgatcattt taaatacggt gtcgaccgaa
    24721 ccaatctctt ctgcatgagc caatggcccc agcattatca ggcttaaaga caggatcaaa
    24781 tgcttgtatt tcatattgtt accattttta tctattacac gagcaattag ttcgtcacgg
    24841 taaataacat attgattaag aacaaaatct caattctttt atttccgtca agccctaaaa
    24901 catactctac taagaaaaaa acactgaatg ctaaaacagc aaaaaatgct attatccaat
    24961 tacctgatgt caggtgctcg ttgttgaaag gatgaggata ttttatggat caggccggca
    25021 ttattcgcga ccttttaatc tggctggaag gtcatctgga tcagcccctg tcgctcgaca
    25081 atgtagcggc gaaagcaggt tattccaagt ggcacttaca gagaatgttt aaagatgtca
    25141 ctggccatgc tattggcgcg tatattcgtg ctcgtcgttt gtcgaaatcg gcggtcgcac
    25201 tacgcctgac tgcgcgtccg attctggaca tcgcgctgca ataccgcttc gactctcaac
    25261 agacatttac ccgcgcattc aagaagcagt ttgcccagac tcctgcactt taccgccgtt
    25321 ctcctgaatg gagcgccttt ggtattcgcc cgccgctacg tctgggtgaa ttcacaatgc
    25381 cagagcacaa atttgtcacc ctggaagata cgccgctgat tggtgttacc cagagctact
    25441 cctgttcgct ggagcaaatc tctgatttcc gccatgaaat gcgttatcag ttctggcacg
    25501 attttctcgg caacgcgccg accattccgc cggtgctcta cggcctgaat gaaacgcgtc
    25561 cgagtcagga taaagacgac gaacaagagg tattctatac caccgcgtta gcccaggatc
    25621 aggcagatgg ctatgtactg acggggcatc cggtgatgct gcagggcggc gaatatgtga
    25681 tgtttaccta tgaaggtctg ggaaccggcg tgcaggagtt tatcctgacg gtatacggaa
    25741 cgtgcatgcc aatgctcaat ctgacgcgcc gtaaaggtca ggatattgag cgatactacc
    25801 cggcagaaga cgccaaagcg ggagatcgcc caattaatct gcgctgtgaa ctgctgattc
    25861 ctatccgccg ttaacgctgc agctcatcta atgcaggggc gtccagatgc gagatgtccc
    25921 ctgcggtttc aacgacccag ccagacgcca gccacaggct ttcctgataa tccacgcgcg
    25981 aaatagaaca gttacgcaga cgtaagcggc gctctgccca tgctggtaat ccgagaatcg
    26041 tactcaccag acatcccagt gcaataccat ggctcaccag cagcggtcgg cttccctgcg
    26101 gtaagtcccg gcaagattcc agtgccgcat tgacgcgatc gctgagctcc tgcattgact
    26161 cgccttcagg aatacgcccg tcaacggtgc cattgaccag ctgccgacgc cagttctctt
    26221 cttcttcggt cagagaatcg atatgtcttt tttccagcac acccatgttt aattcacgca
    26281 ggcgagaatc aaagatgatg tcacagccgc aggcctgggc gatgatttcc gccgtacgcc
    26341 gggtgcgtcc taaatcgcta ctgataatat gcgtaatgcc aagctctttg gcacgggttg
    26401 ccacctgcat cgcctgttgc tcacctttgg cggtcagcgg gctgtcagac tggccctgaa
    26461 tacgtcgctc ggcgttccac tgcgtttcac cgtggcggac taggtatacc tgtaacatgc
    26521 tttttttccg tatactgctg taacgttaat tttctgagta atgctgatta tgcaccaagt
    26581 tgtctgtgcg accaccaatc ccgctaaaat tcaggccatt ctgcaggcat ttcacgagat
    26641 cttcggcgaa ggatcctgcc atattgcatc cgttgccgtc gagagcggtg taccggaaca
    26701 accctttggc agtgaggaaa cgcgcgctgg cgcacgaaat cgggtagcaa atgcccgccg
    26761 tttacttcca gaggctgatt tttgggtggc gattgaagct ggcattgatg gcgacagcac
    26821 tttcagttgg gtggtgattg aaaacgccag ccagcgcggc gaggcgcgtt cggcaacttt
    26881 gccgttacct gcggtgattc tggaaaaagt tcgtgaaggc gaggcgcttg gtcccgtgag
    26941 tcgcgttata ccggtattga tgagattggt cgtaaggaag gggcgatcgg cgtgtttacc
    27001 gccgggaaac tcactcgcgc cagcgtctat caccaggcgg tgatcctggc gctgagtccg
    27061 tttcacaatg ccgtgtatca ggcattgtag gacggataag gcgttcacgc cgcatccgac
    27121 attcggtgca cgatgcctga tgcgacgctg ccgcgtctta tcaggcctac aaaatcaatc
    27181 gcttttcagc aacacctctt ccagccactg acgcagctcc acgggcgcgg ctttcaggct
    27241 gttagatcca cgcgtaatcg tcgcgatgcc cgcgccgagt tcatttttta actcacgctg
    27301 actcatttcg ccacgcaaca gctcttcgac aatacgcacg cgagtcccca acgcttcgcg
    27361 ctcatctggc gtcagcatca ggtttaacaa cggtaaatgg agatcgtttt ggtaggcatt
    27421 cttaagcagg tcgacaaaac gtaaccactc ctggtgacgc tgttctgcca tcgctgctga
    27481 atagggtgat tgttgggcca taatatgtcg ccattgttag cgggggaagc aaaatgactc
    27541 ccccggttgt actcgctaaa gagtacgata gcatatcata aacgtgcgga tcagtaacga
    27601 cgtccccatt ccgtggcgct catcaacgtc ggtttatccc ccatgaaata gcggtagtaa
    27661 gcatcataag ccagcacgtt cttcacataa ccgcgcgtct cggagaacgg aatactctcg
    27721 acaaatgcca cagcgtcgat acgcccggcg ctgttgccaa gccaggttcg cacccgtcca
    27781 ggtccggcgt tataagctgc tgaggagaaa atacggttat tgccaaactg ctgataaaca
    27841 tattgcaggt aactggtgcc aatgttgatg ttcgtttccg gatccagcaa ttgcccaggg
    27901 ctgctataac caggaataga gaacatcttc accgtatggg tcgctgtacc aggcataatc
    27961 tgcatcaggc cgctggcccc taccggtgat ttcactttcg gattccaggc gctctcctga
    28021 cgggcaatcg ccatcgcata gctttgcggg atctctttcc cactggtgta acgtttgaaa
    28081 agatcgttgt aagccagcgg gaatcgctct tccagatgat cccaaagctt cccggcgatc
    28141 gttgcctgaa cgctaagatc ccaccattgg ttattgaaag cataccgagc cagttgagcc
    28201 tgctctgttt ttgacttgct cttcaccaga ttggcccact cgctacgcgc ggtattatcg
    28261 agattccagt acatcaactc gcgcacgcgc gccatctccg gcccctgagt cagggtgctg
    28321 tcaacattct gcggcgcttt atcaatcttc agctcatact cttcaccgat gcgttgtgct
    28381 gcaaccatcg ggtagaaacc acgctgttgt atgagttgat gcaaaatctc tttcgcttca
    28441 gcttcacgtc cgcgttccag caataaatcc gcctgccagt aacgccattc atctttctct
    28501 tttgcttcca tcggcagacg cgccagccag gtattcaggc cgcgacgatc gccggtgcca
    28561 agcgccattc gcacacggcg ttcaatcaac gacgtggatt gcgagcgcat aatggcgtca
    28621 tcgcgccatt tcgcctgctc atcggtgaca tcattgccca tcaaacgcca ggcgacgata
    28681 tcgcgcagct cctgaatctg atcttcatta agctgctgcg cctgggcaag cgacgggatc
    28741 atcagccgtg cattttcggc atcctgccgc gccacactgg caaacgccac cgccgccatt
    28801 tgacgggtaa aatcggtcgc gccggttgta cgcgcgaagg tcagtaccgt attagggttg
    28861 ttcgccagtg aaatgattgc cgaggcgata gtctggtaat cggcaggcat ctgccctgcc
    28921 agcactgtta ccaggcctgt gttacccgct ttcatcgcca gacggatacg ctctaaatac
    28981 gccagcggat cttgtttacc tgacgcacgc cagacgctaa ataacttgtc acaggcgtta
    29041 ggctggctct tgccggttag ccacagctct ttcgcccctt gccaggcttc ttcactctgc
    29101 ccggtgttcc atttcgcata gtagtaatta cattgcgctt cggtagttcc gggcttttcc
    29161 gggctaaagg ctaacaagcc acgccagtct tcacgccgcg ccagttcatt gacgaaacga
    29221 gattgcagcg tgcgagcggg aggaagcgtg gggttagcgc gaacaaagtt agtgaccgtc
    29281 accgccggtt gattcatcag gtcatcggtg atctggcggt attccaggta gggataaagc
    29341 ggataatcct tcagtccagg catcatttgt tcgaccacat ccatctgtcg attatcccag
    29401 gcctgcttga tttgcgcgta acggctacgc tgctcatcca gtgagtcggc tcgcgccacg
    29461 ctgctgaccg tcagcagaca gacaccggca gccaacagcc gccaggtaac ttgtttggct
    29521 ttttccacaa gcgcatcctc taagtgtaaa tacatcaatg cagcgtcatg ccgcgtaata
    29581 gatttatgct aaccagtcat tgccgtttac gccacgttac ggacactttt ttacttttac
    29641 tgcgaaggtg atcgaccaac tgggattagg cggggaaaca ggctacactt cgcctttgaa
    29701 aactcatcat cacgataaac atagaggcga agtccaacgt ggctcaattc gtttatacca
    29761 tgcatcgtgt cggcaaagtt gttccgccga aacgtcatat tttgaaaaac atctctctga
    29821 gtttcttccc tggggcaaaa attggtgtcc tgggtctgaa cggcgcgggt aagtccaccc
    29881 tgctgcgcat tatggcgggc attgataaag acatcgaagg tgaagcgcgt ccgcagccag
    29941 acatcaagat tggttatctg ccgcaggaac cgcagctgaa cccggaacac accgtgcgtg
    30001 agtccattga agaagcggtt tcagaagtgg ttaacgccct gaaacgcctg gatgaagtgt
    30061 atgcgctgta cgccgatccg gatgccgatt ttgacaagct ggccgctgaa caaggccgtc
    30121 tggaagagat cattcaggct cacgacggtc ataacctgaa cgtacagctg gagcgtgcgg
    30181 cggatgcgct gcgtctgcca gattgggacg cgaaaatcgc taacctctcc ggtggtgagc
    30241 gtcgtcgcgt ggcgttgtgc cgcctgctgc tggaaaaacc agacatgctg ctgctcgacg
    30301 aaccgaccaa ccacctggat gccgaatccg tggcgtggct ggaacgcttc ctgcacgact
    30361 tcgaaggcac cgtggtggcg attactcacg accgttactt cctcgataac gttgcaggct
    30421 ggatcctcga actggaccgc ggtgaaggaa ttccgtggga aggtaactac tcctcctggc
    30481 tggagcagaa agatcagcgt ctggcgcagg aagcttcaca agaagcggcg cgtcgtaagt
    30541 cgatcgagaa agagctggag tgggtacgtc agggaactaa aggccgccag tcgaaaggta
    30601 aagcacgtct ggcacgcttt gaagagctga acagcaccga ataccagaaa cgtaacgaaa
    30661 ccaacgaact gtttattcca cctggaccgc gtctgggcga taaagtgctg gaagtcagca
    30721 acctgcgtaa atcctacggc gatcgcctgc tgattgatga cctgagcttc tcgatcccga
    30781 aaggggcgat cgtcgggatc atcggtccga acggcgcggg taaatcgacc ctgttccgta
    30841 tgatctccgg tcaggaacag ccggacagcg gcaccatcac tttgggtgaa acggtgaaac
    30901 tggcatcggt tgatcagttc cgtgactcaa tggataacag caaaaccgtt tgggaagaag
    30961 tttccggcgg gctggatatt atgaagatcg gcaacaccga gatgccaagc cgcgcctacg
    31021 ttggccgctt taactttaaa ggggttgatc agggtaaacg tgttggcgaa ctttccggtg
    31081 gtgagcgcgg tcgtctgcat ctggcgaagc tgctgcaggt tggcggcaac atgctgctgc
    31141 tcgacgaacc aaccaacgac ctggatatcg aaaccctgcg cgcgctggaa aacgccctgc
    31201 tggagttccc gggctgtgcg atggttatct cgcacgaccg ttggttcctc gaccgtatcg
    31261 ccacgcacat cctggactac caggatgaag gtaaagttga gttcttcgaa ggtaacttta
    31321 ctgagtacga agagtacaaa aaacgcacgc tgggcgcaga cgcgctggag ccgaagcgta
    31381 tcaagtacaa gcgtattgcg aagtaatgcg taaaatgccg gatgcggcgc gaacgcctta
    31441 tccggcctac aaaaccagca atttcaatac gttgtaaaac tgtaggcctg ataagacgcg
    31501 gtaagcgtcg catcaggcat tgagcaccga atgccggatg cggcgtgaac accttttccg
    31561 gtctacaaca gccttagcat cctcaatttc gtggcgggtc acccgtgcag gttttttcaa
    31621 accttgttta atctcgacag cctcttccag agaggttttc aactcatcaa aaaaactcat
    31681 tttatacctc gcctttgagc agtttcgtca cggttatcct tgctccccca tcatctcccg
    31741 caccagctcc acgcagcgca ggaaacggct gtcgtaatcg tcctcttcaa cccgcacgaa
    31801 ttcgatattg ttctcttcca gcatctccac cagcaagttc tggaactctt tgcgatccac
    31861 cgaactgccg aggctgcgta aaccatccgc cacccacggc gtgttgttct ccagcaggat
    31921 caccagatcg aaacggtatt catcaatcag cgcctgcacg aacggatgct cacgcccttc
    31981 gtactttttg cagaacgcct gggtagtgac aaaatcggtg tcaataaacg ccactttatt
    32041 ggcgtatttc actgcaaaat caatgtattg tgcgtgaccc aacgcgattt tatcgtagtc
    32101 ggaatactgc aatgcgatct catcaccgcc gaggtgtgaa aagacataat cgcggccata
    32161 ttcccacgca ctggtggtgt tgaagatatt ggcaagtttg tttaccaggg tggatttacc
    32221 gctcgactcg ccgccaagga tcgccacggt acgcacaaaa aacggcttca cttcggtagg
    32281 aatatattcc cagtagcgga acgggttttc gcggatctgc gcaccgctga tactcataaa
    32341 ggtacgtttc ggatcgacca gcaccgtctc gatccccaga tgttccatat actgtggcgc
    32401 atcggcttct tccgaggtgt agatcaggtc cggctggatc cccttttcag ccataaactt
    32461 tttgatgccg ttgctccaca catcccagcc atgcggatac ggctccatgc cctcttcgtt
    32521 gaaagcatga atacggatat ttttctgata tttaaaggtt tgcaatagcc agcgcagacg
    32581 atccggcacc gtgggctgct gcgacatggc gctgtcttca aacaacgcgc ggtcgcgggt
    32641 atcgtcaaaa cccataatga tatgcagctc gtcaacctgg ctacaggcgc gctggataag
    32701 gtagatatgt ccggtatgca gcgggtagaa cttaccgaat acgacaccaa tggttttctt
    32761 ctgccgggga aattcaagcc ccaaaaaacg gtgcaatgcc tccagctttt gcgcgctggg
    32821 gcttttaatt ttggcattca gtaactggct taaataccct ttggtcatac cgctggcatc
    32881 agctacctgc tgtagcgtgc agccctgttg cttgatggca gttttcaggt aatcaaatga
    32941 cgacatatct ccctccgtat ctctcattat aagtcgtcga acacgctaag cgcgtcggag
    33001 agttttttaa cgccaaaaat ctgcatccct tccggcgctt ttttcggtac gttagccgcc
    33061 ggaacaatcg cccggcgaaa accgtgtttc gccgcttcag aaatccgctc ctggccactg
    33121 ggcaccgggc ggatctcccc tgccagcccg acttcaccaa acaccaccag atcctgtggc
    33181 agcggtctgt cacgcaggct ggaaaccatc gccagcagta acgctaagtc ggcgctggtt
    33241 tcggttacct tcacgccgcc gaccacgttc acaaacacat cctgatcggc catttgcaga
    33301 ccaccgtgac ggtgcaagac agccagcagg attgccagac ggttttgttc cagccccact
    33361 gccacgcggc gcggattcgc cattatcgag tgatcgacca gcgcctgaat ctccaccagc
    33421 aacggacgcg ttccttccca caccaccatc acggagctgc cggaggtcac ttcatctccg
    33481 cgacttaaga aaattgccga agggttgctg acttcacgca gcccctgttc ggtcatcgcg
    33541 aagacgccca gctcattcac cgcgccgaag cggtttttat ggctgcgtaa ggtgcgaaaa
    33601 cgggagtcgg catcgccatc cagaagcacc gaacagtcga tacagtgttc cagcactttc
    33661 gggccagcca gcgaaccatc tttggttacg tgtccaacca tgacaatcgc cacaccgcgc
    33721 gttttggcga aacgcgtcag ataagccgcc gtttcacgca cctgcgccac gctaccaggc
    33781 gatgattgta catccgccat atgcatcacc tggatcgagt caattaccat cagcttcggt
    33841 tgctcttctt cggcaatcag gcagatctgc tcgatgctgg tttccgacaa catattgaga
    33901 ttgtcagtcg gcaggccaag gcgatgagcg cgcattgcca cctgttgcag cgactcttcg
    33961 ccggtgacat acagcgtttt catctgctgg gccagtttgc acagcgtttg cagcaacagc
    34021 gtggatttcc ccgcaccagg gttaccgcca atcagaatgg cacttcctgg caccacgccg
    34081 cctcctagta cgcggtcgaa ctctttaaat ccggtggaaa aacgcggcag ctcttcaagg
    34141 ctgatatcgg agagtttctg gacttttgcc accccggcgc taccggcata gccgctgaga
    34201 cgctcgttac gcgccaccgt tggcgacgca gcaagacgca cctcggtgat ggtgttccag
    34261 gcatgacagg cactgcactg cccctgccag cgcggataat cggccccgca ttcattacaa
    34321 acaaaggcgc gttttggagc ttttgccacg gtgtacctcg ttaatgctgt gccgcccgca
    34381 ggatgacggg caggcaatta cttctgattc aggctgccag agaggatgca gaataccccc
    34441 atcaggtcag cgtgacggat ggtgacttcc gccttttcat tcacttttgg cttggcatgg
    34501 taggcaatcc ccagccctgc tgctttgatc atcggcaggt cattagctcc atcgccaatc
    34561 gccacggtct gcgccagcgg gatttcatac tcctgcgcga ggcgagtcag agttttcgct
    34621 ttgtactgcg cgtctacgat gtcgccgatc acattgccgg taaatttacc gtccatgatc
    34681 tccagttcat tggctaccac ggctgtcagg cgcagcttgt cgcgcaggta ttcagcaaag
    34741 aaagtaaagc cgccggaggc aatcgccact ttccagccca gcgtttccag cttgagcacc
    34801 agctgcgtta agcctggcat cagcggcaga ttttcacgca cctgttgcag aatattggcg
    34861 tcagcgcctt tcagcgtcgc cacgcggctg cgcaggctgg cggtaaaatc gagttcgccg
    34921 cgcatcgccc gttcggttac ctccgccacc atctcgcccg ttccggccag cttggcaatt
    34981 tcatcaatac attcaatctg gatggcggtg gaatccatgt ccatcaccag caaacccggc
    35041 gtgcgcaggt gcgggatttt ccccagcggg gcgacatcca gctgcgcttc gtgggccagg
    35101 cgtgtagccc gtgcggtgag tgaacctgcc agacgaatca cctgataatc ttccacgcac
    35161 caggcggcaa caatcaccat cgccgcaccc agtttgctct ggtattgggt cagacgctgt
    35221 ttatccagcc cacgaccata cagcagccag ccgctacgac ctgcgtggta atccagtggc
    35281 atcacttcat caccacttaa tgaaagaggc agacccggcc ataaagagac atcttcaggc
    35341 aggtcgcacc aggtaatgtt aggcattaag gctcctgtaa aatcgttcga agcagggaaa
    35401 ataacgcatg aggctacctt gtatccattg cttctggcaa cattaagtct caaattttca
    35461 aagggtggaa gatggctcgc acaaaactga aattccggct acatcgggca gtgattgtcc
    35521 tgttctgtct tgccttgtta gtggcactga tgcagggggc gtcatggttt agtcaaaacc
    35581 accagcgaca gcgtaatcca cagctggaag aactggcccg caccctggcg cgtcaggtga
    35641 cgctgaacgt tgcaccgctg atgcgtaccg actcaccgga tgaaaaacgc attcaggcga
    35701 tcctcgatca gttaacagat gaaagccgta tcctcgacgc gggtgtgtat gacgaacaag
    35761 gcgatcttat cgcacgttct ggcgaaagcg tcgaagtgcg cgatcggctg gcgctcgacg
    35821 gtaaaaaagc aggcggctat tttaaccagc agattgtcga gccaattgcg ggtaaaaacg
    35881 gaccgctcgg ctatctgcgc ctgacactcg acacccatac gctcgccacc gaagcccaac
    35941 aggtggataa caccactaac attttacgcc tgatgttgct gctctcactg gcaatcggcg
    36001 tagtgctgac ccgcacgctg ctacagggta aacgcacccg ctggcagcaa tcgcccttcc
    36061 tgttaaccgc cagcaaaccg gtgccggaag aggaagaaag cgagaaaaaa gagtgaccca
    36121 ttactacaag aaaggaaatc gttatgtcca cattacgcct gctcatctct gactcttacg
    36181 acccgtggtt taacctggcg gtggaagagt gtatttttcg ccaaatgccc gccacgcagc
    36241 gcgttctgtt tctctggcgc aatgccgaca cggtagtaat tggtcgcgcg cagaacccgt
    36301 ggaaagagtg taatacccgg cggatggaag aagataacgt ccgcctggcg cggcgcagtg
    36361 gcggtggcgg cgcggtgttc cacgatctcg gcaatacctg ctttaccttt atggctggca
    36421 agccggagta cgataaaact atctccacgt cgattgtgct caatgcgctg aacgcgctcg
    36481 gcgtcagcgc cgaagcgtcc ggacgtaacg atctggtggt gaaaaccgtc gaaggcgacc
    36541 gcaaagtctc aggctcggcc tatcgcgaaa ccaaagatcg cggcttccac cacggcacct
    36601 tgctactcaa tgccgacctc agccgcctgg caaactatct caatccggat aaaaagaaac
    36661 tggcggcgaa aggcattact tcagtgcgtt cccgcgtgac caacctcacc gagctgctgc
    36721 cggggctcac catgagcagg tttgcgaggc cataaccgag gcctttttcg cccattatgg
    36781 cgagcacgtg gaagcggaaa tcatctcccc ggacaaaacg ccagacttgc caaacttcgc
    36841 cgaaaccttt gcccgtcaga gtagctgaga gtggaacttc ggtcaggctc cggcattctc
    36901 gcatctgctg gatgaacgct ttagctgggg cggcgtggaa ctgcatttcg acgttgaaaa
    36961 aggccatatc acccgcgccc aggtatttac cgacagcctc aaccccgcgc cgctggaagc
    37021 cctcgccggg cgactgcaag gctgcctgta ccgcgcggat atgctgcaac aagagtgcga
    37081 agcgctgttg gttgacttcc cggaccagga aaaagagcta cgggagttgt cgacgtggat
    37141 agcgggggcg gtaaggtaac tcgctgatta aaatatccaa ggaaggaaat atgatcgatc
    37201 tggaaaacca ggaacgggag attatcaatt taatgttcag ccagcgtatt tcctggctag
    37261 ccgccgtgcg tatcaggcat aaattatcgc ttgctgaagt aagtaaaatg ttaggtatct
    37321 cgattaattc tctaaaacag atcgaaaaga cggaacggct tagctcaaac atcaaaagca
    37381 aaatggcaga aatttatggt tgtccaccag agttgctgat atgcccttcc tggatgacgg
    37441 ctgaacataa atgaatagat tcgtgaatag atacttgaat gttttttatt ttctatccat
    37501 atgaaaaata atagtaaatc tataaatcag ttgctattga tgaatagata ctaaaaagcc
    37561 ggagcagtcc cccactccgg cttaacacga caatcgcctt tcagcgaaac acaattactc
    37621 tttatcgccc agcagaacgg attccagtgc gattttgatc atgtcgttga aggtggtctg
    37681 acgctcagcg gcagtggtct gctcgtgagt gcggatgtgg tcagacacgg tgcagatggt
    37741 cagggctttc gcgccaaact ctgcagccac gccgtagata ccagccgctt ccatttccac
    37801 gccgaggatg ccgtattttt ccatcacgtc gaacatttcg ccgtccggag agtagaacag
    37861 gtcagcggag aacaggttac ccacgcgagc atcaacgccc agtgctttag ctgcatctac
    37921 tgcgttacgc accatgtcaa agtcagcgat agcggcaaag tcatggtctt taaaacggat
    37981 gcggttaact ttggaatcgg tgcaggcccc cataccgata acgacgtcgc gcagttttac
    38041 gtgcggcaga actgcgccac aggaaccaac gcggataatt ttcttcacgc cgaaatcggt
    38101 gatcagttct ttggtgtaga tggagcagga cgggataccc ataccgtgac ccattacgga
    38161 aattttgcgg cctttgtaag taccagtgaa gcccagcata ccgcgaacgt tgttcacttc
    38221 acgggcatct tcaaggaaag tttcagcaat atacttcgca cgcagcgggt cgcctggcat
    38281 caaaactacg tcagcgaaat cgcccatttc tgcattaatg tgtggggtag ccattgtttt
    38341 atcctttttg tgacataaca aaggcggagt caacgctccg cccaaatcca tcagaacatg
    38401 gctttgccat attccatatc agaagtacca aaatattttg ccagagtctg gccgatatcc
    38461 gcgaaggttt cacggtgacc cagtgagccc ggttttactt tcgggccata taccagtacc
    38521 ggaatgtgtt cacgcgtgtg gtcagtaccg gtccaggtcg gatcgcaacc gtggtcagcg
    38581 gtgaggatca ggatgtcgtc atcgcgcagc agagacatca gctccggcag acggcggtcg
    38641 aacagttcca gacccgcagc ataaccggcg acgtcgcggc ggtggcccca ggaagagtcg
    38701 aagtcaacga agttggtgaa gacgatggtg ttatcacccg cttctttcat ctctttgatg
    38761 gtggcgtcaa acagcgcgtc caagccagtc gctttcactt ttttggtgat accgcagttg
    38821 gcgtagatgt ctgcaatttt accgacagaa accacctggc cgtgtttttc atcaaccagt
    38881 ttctgcagca cggtcggtgc tggcggctca acagccaagt cgtgacggtt accggtacgc
    38941 tggaagttac cagctttgtc accgataaac ggacgagcga taacacgacc gatattgtag
    39001 ccgccgttgg tcagctcttc acgggcgatt tcgcacagtt cgtagagttt atccagaccg
    39061 aaagtttctt catggcaggc aatctggaac acggagtcag cggaggtata gaaaatcggc
    39121 ttgccggttt tcatgtgctc ttcgcccagt tgatccagaa tgaccgtacc ggaagagtgg
    39181 cagttaccga ggtaacccgg cagattagcg cgttcgacca gtttatccag cagctcttgc
    39241 gggaagctgt tttcgtgatc ggagaaatat ccccactcaa acagaaccgg gacaccggcg
    39301 atttcccagt gaccagacgg ggtatcttta ccggatgaca tttcgtgcgc ccatgcgtac
    39361 gcgccgataa cttcagcgtt gccgtccatt cccgccggaa tgaaaccggt agaaccttcg
    39421 tgtgctttcg ccagccccag acgggtcaga tttggcagat tgagcggccc tttacgacca
    39481 ttatcagctt cgcctttggc acaagcttct gcgatatgac ccagggtgtc agccccgacg
    39541 tcaccaaagc gttctgcatc ttctgtagcg ccgatgccga atgagtccag caccataata
    39601 aatgcacgtt tcatatgttc tccgtactca gtgcttcaaa tttaaatgat cagatcagta
    39661 taccgttatt cgctgatacg gcgatagaca gttggtgtgc tttccggtgc gttatcggca
    39721 agtttaattg ccgctttcac cgctttcgcc gcttcctgcc agctgttttc gtctttcgcg
    39781 aggataaccg ccagcggacg ctgaccgtct acctggtcgc ccagacgcgc catatcagta
    39841 aagccgacgc tgtaatcgat ggtgtcagat gcctgacgac gaccgccgcc cattgcaact
    39901 actgccatcc ccagcgcgcg ggtatccatt tcactgacaa aaccttcggt atcagcatag
    39961 actgctttcg tcagcatcgc tgtcggcaga tacttcgcgt agttctcaac gaagtcggtc
    40021 gggccttttt gtgccgctac catacgacca aagacttctg ccgctttacc gttgtccagc
    40081 accgcctgca atttcgcgcg cgcttcggcg tcatctttcg ccagtttgcc ggagatcaac
    40141 atctccacgc acagcgccat cgtgacatca aacagacgcg ggttacgata ctcacccgtc
    40201 aggaactgca ccgcttcacg aacttcaacc gcgttacctg cactggaggc cagtacctga
    40261 ttcatgtcgg tgagcagcgc ggtggtgcgc acgccagcgc cgttagccac gccaacaatc
    40321 gcttcggcaa gggcttcaga gagttcgtag gtcggcataa acgcgccgct acccactttc
    40381 acgtccatca ccagcgcgtc cagaccttcc gccagtttct tcgccaggat cgaggcagtg
    40441 atcagcggga tggagtccac ggttgcggta atatcacggg tcgcgtagaa acgtttatca
    40501 gccggagcca gtgaactggt ctgaccgata atcgccacgc cgacgtcttt aataatttcg
    40561 cggaaacggt tgtcatccgg gaaaatgtcg aagccaggga tggattccag tttgtcgagc
    40621 gtaccgccag tatgaccgag gccgcgaccg gagatcatcg gaatatagcc gccgcaggct
    40681 gcgaccatcg gccccaacat cagcgaagtg acatcgccga cgccgccggt cgagtgttta
    40741 tcaacaatcg ggccattcag atgcaggctt ttccagtcga gaacggttcc tgaatctcgc
    40801 atcgccatgg tcagcgagac acgctcaggc attgtcatat cgtggaagaa aatggtcatc
    40861 gcgagggcgg caatctgccc ttcggaaata gtgttgtcgc gaataccgtt gataaagaaa
    40921 cgaatttctt catcgctcag cgcatgacca tcacgttttt tacgaataat ttcttgtgcg
    40981 agaaacaagg taacctcccg aggaaaagag tatgaggcgg cttttgccgg atgcgtctac
    41041 gccttatccg gcctacgggt agatacgtct cgtaggcctg ataagcgcag cgcatcaggc
    41101 gtaaagcatc ttacttagta gctgctggcg ctcttaccgt cgccgtggcc cagcgctttc
    41161 agcaggcttg ccagcaggct ggaagcacca aagcggtagt gacgcgcatc agcccagtca
    41221 gcaccgaaca gttcatctgc gatggcgaga tatttctgcg catcttccgc agtacgcacg
    41281 ccgcccgccg gtttgaaacc aacggttttt tctacgccca tatcacggat cacttccatc
    41341 atgatgcgag cgctttccgg cgtcgcgttc acagccactt taccggtaga ggttttgatg
    41401 aagtccgcac ccgctttgat ggagatttca gacgctttac ggatcagcgc ttcgtctttc
    41461 agttcgccgg tttcgatgat cactttcagc agtacattcg ctgccgcgca agcctcttta
    41521 caggctttca ccaggtcaaa accaacctgc tcgtcacccg ccatcagcgc gcggtacggg
    41581 aacaccacgt caacttcatc agcaccgtag gcgattgccg cacgggtttc tgccagcgcg
    41641 atttcgatgt cgtcgttacc gtgtgggaag ttggttaccg tagcgatacg gatttccggg
    41701 gtgccctgct ctttcagagt tttgcgagca atcgggataa aacgaggata gatacagata
    41761 gcggcggtat tgccgaccgg agttttggcc tgatgacaca gagcaattac tttctcgtcg
    41821 gtgtcgtcgt cattcagggt ggtcaggtcc atcaatttca gtgcacgcag gctgcttgct
    41881 ttcagatcag tcatttcatt ctcctggctt gtcgccaata aaattcacct tgcgagtttg
    41941 ttagtattct aacatctact ccgcaacaca cttcgataca catcacaatt aaggaaatct
    42001 acttacaagt ttgcatcact gtaatgcgat ctggttcaaa taattcactt tcaaatgaat
    42061 gcgtcagtgg tggcaaacgc atcaggatca aagtgaacat cacgaaactt cttacaatgg
    42121 cgcatcacct tcggcataaa aggaaaagaa gatgcccact tctcatgaaa atgcactgca
    42181 acaacgttgc cagcaaattg tcaccagccc agtgcttagc ccggagcaga agcgccattt
    42241 tctggcactg gaagcagaaa acaatctgcc ttacccacag cttcctgccg aagcccgccg
    42301 cgcgctggat gaaggtgtaa tctgcgatat gtttgaaggt catgcgccgt acaaaccgcg
    42361 ctatgtctta cccgattacg ctcgttttct ggcgaacggt tccgaatggc tggagctgga
    42421 aggcgcgaaa gatcttgatg acgcactctc tctgctgacc attctttacc accacgtacc
    42481 gtcggtcaca tcgatgccgg tctacctggg gcaactggat gcgttgttgc aaccgtatgt
    42541 tagaattcta acacaaaacg agatcgatgt tcgaataaaa cgtttctggc gttacctcga
    42601 cagaaccctg ccagatgcct ttatgcacgc caatatcggc ccgtctgatt cgcccattac
    42661 ccgcgcgatc ttacgtgcag acgcgcaggt ttcaccgaac ctgaccttta tctacgatcc
    42721 tgaaatcacc cctgatgacc tgctgctgga agtggcgaag aacatctgtg aatgtagcaa
    42781 accgcacatc gccaacggtc cggtgcatga taaaattttc acaaaagggg gctacgggat
    42841 tgtgagctgt tacaactcac tgccgctggc gggtggtggc agcacgctgg tacgccttaa
    42901 cctgaaagcc attgccgagc gcagcgaatc gctggatgac ttctttacgc gcactctacc
    42961 gcactactgc cagcagcaga tcgccatcat cgatgcgcgg tgtgaattcc tctatcaaca
    43021 atcacacttc tttgagaata gcttcctggt gaaagaaggg ctgattaacc ctgaacgttt
    43081 tgtgccaatg tttggcatgt atgggctggc ggaagcggtt aacttgctgt gtgagaaaga
    43141 agggattgcc gcgcgctacg gtaaagaagc cgccgcaaat gaagtgggtt atcgcatcag
    43201 cgcgcaactg gcggagtttg tcgccaatac ccccgtgaaa tatggctggc aaaaacgcgc
    43261 catgttacac gcacagtcgg ggatcagttc cgatatcggc accacgccgg gcgcgcgttt
    43321 gccgtatggc gatgagccag atccgatcac ccatctgcaa actgtcgccc cgcatcatgc
    43381 ttattattat tccggcatca gcgacattct gacgctcgac gaaaccatca aacgtaaccc
    43441 gcaggcactg gtacagcttt gcctcggtgc ctttaaagcc ggaatgcgtg aatttaccgc
    43501 caatgtcagc ggtaacgatc tggttcgcgt taccggttat atggtgcgtt tgtcggattt
    43561 agaaaaatat cgcgccgaag gttcacgcac caacaccacc tggctgggcg aagaagccgc
    43621 acgcaacact cgtattctgg aacgccagcc gcgcgtgata agccatgaac agcagatgcg
    43681 ctttagtcag taagattatc cccttctcct gcgttgacgg gccaggcagt cgtctggctc
    43741 tgtttttgca gggctgcaat ctgcgctgca aaaactgtca caatccgtgg acgatgggac
    43801 gttgcaatga ctgtggggag tgcgtgccac agtgtccgca tcaggcgttg cagattgttg
    43861 acggcaaagt ggtgtggaac gctgtggtct gcgagcaatg tgatacctgc ctgaagatgt
    43921 gtccgcaaca tgccacacca atggcgcaat ccatgagcgt ggacgaagtg cttagccatg
    43981 tgcgcaaagc ggtgctgttt attgaaggga taacggtaag cggcggcgaa gccacgaccc
    44041 aactgccgtt tgtagtggcg ctgtttactg ctatcaaaaa cgatccgcaa ctgcgccatc
    44101 tcacctgtct ggtggacagt aacggtatgt tgagcgaaac cggctgggaa aaattactcc
    44161 cggtgtgtga cggcgcaatg ctcgatctca aagcatgggg tagcgaatgt catcaacaat
    44221 taaccggacg cgataatcaa cagattaagc gcagcatcta tttgctggca gagcgcggca
    44281 agctggcgga actgcgtttg ctgatgatcc caggccaggt ggattatttg caacacatcg
    44341 aagaactggc ggcgtttatc aagggacttg gtgatgttcc ggttcgcctg aacgcgtttc
    44401 atgcccacgg cgtgtatggc gaggcgcaaa gctgggcgag cgccacgccg gaagacgttg
    44461 agccgttggc tgatgcatta aaggtgcgcg gggtgagccg gttgatattt ccggcgctct
    44521 atttgtgaca tgccggatac ggcctacggt tcggcacaga cttgtaggcc tgataagacg
    44581 cgtcaagcgt cgcatcaggc attgtgcgcc aactgccgga tgcggcgtga acgccttatc
    44641 cggcctacgg ttcggcacaa acctgtaggc atgataagac gcgttaagcg tcgcatcagg
    44701 cattatgcgc cgactgccgg atgctgccta cgaagggcaa actacccacg aacgttaaac
    44761 accgcatacg tgttattaag caacacttcg gcaatctcat ccgcgggttc cgggcgtaac
    44821 tcgcaaagca cggcaaacac acgggcagcc tgttccgggc ggttaggctg cccctgaaaa
    44881 ccgttgagcg gcatatccgg cgcatcggtt tccagcaaca acgatgccag cggtaatttt
    44941 gcgatgacat cgcgggtttt actggcgcgt ggataggtga tagtaccgcc tacgccgatt
    45001 ttgtagccca gctgtacaaa ccgttcagcc tgttgctggc tgccagaaaa tccgtggaca
    45061 acaccagcac gcggcaaatc gtggcgttta agatgcatcg ccagtttgtc gtgcgtgcgc
    45121 cgtgaatgca ggatcaccgg cagatcgtag cgtttcgcca gtttcagttg ttcgtcgagt
    45181 aaccactgct gcctctcaaa ttgcggatcg tcgccgaaga gatccagacc gatctccccc
    45241 accgccacca ccttcgccgg acgcctttcc agcgcctgct gtagctgctc aagagacaca
    45301 tcgctatgtt tttccaacat accaggatgc aagcccaata cggcatacag cggttgataa
    45361 ttttccgcta acgcctgcac acgggcaaaa ttttccgcct ctgtcgccgg aacaatgatc
    45421 ttgcctacgc ccgcttgtgc cgcgcgttgc aggctggcct cttcatcgcc aataaaaggc
    45481 gggaaatcaa aatggcagtg agtgtcgata aaacggcaaa tcaagccaga tcctcattgt
    45541 taaatgtcgg gtcgttcgcc tgcggagcgt cgctcacttc ggcaaccagc gtatcgttgg
    45601 cgactggcgc aggcggaatg acaatcgatt ccggcgtcac cactggcacc agatggcggg
    45661 taagcggcgc tttttcagtt aatagcttgc caaccgtggc gaggaaataa cgaccgcata
    45721 aacgcccaag tttatagtct tcacgcagcg ccggaatccg actgccaagc gcgatactat
    45781 gtaatggctt cggcggataa atttcggata tccgcagctt gcccggtggt ttctcaataa
    45841 attgctgaat gtcacgatag ctggtttcat gatgctgcac cagattgacc agcggctgca
    45901 ggctactgtc acccagccag cgttccatgc gtttgaacca ctgcggcgtg tagtacattt
    45961 gtgacggcac agtgcggatg acgaccaacg ttttagcgcc ctgccttgcc gcctctttaa
    46021 ccggaatcgc atcactgatc ccgccatcca ggtagttaat gccttccagc gacactccgc
    46081 tacgataaaa gccaggtatc gcactggagg cgcgaatcac atccagccag ttttgtttgg
    46141 ttggtaaaaa gtaattcggc gcgtagtcat cctgacgaca ggcgcacata taaaacgatt
    46201 tgccgctgtc aaacaaccgc gcggcggtat ccatttgcaa cggcatctgg cttgcggtgg
    46261 cctccaccag ccagtcgaga tcgataagat ttcctccacg gacaaagcgc aatggatcga
    46321 aaaattcgcg ttttgtggta tagcgcatga tgactttgcg cgcgtaaccg ggctgattgc
    46381 agataaacgc cgagaggttc tgcgccccgg cagatgtgcc gagataaaga tcgaaaggat
    46441 taaactgcgc gcgcataaac tcatccagca cgccagccgt gaaaattcca cgctgtccgc
    46501 cgccttcgca caccagagct attcgtccag gctggaacgg ccttagcgac aacggcgcaa
    46561 tattaccaag cgttacaggt attcgctgcc ccacttctgc cttcctgttt ttgttgtgaa
    46621 agaaagacaa agtaacgcaa tttattattc gctaaaaccg caaaagccag tccgctggac
    46681 tggctttgga taaaattttc ttaatggtct gaaatctagg gtcgttttcg gcccatgaac
    46741 aaactcacta ggaacagaat aatcccgacg acaaagacaa ttttagctgc gcctgcagcg
    46801 gtaccggcca gaccaccaaa cccaagtgcg gcggcgatta acgcgataac cagaaatatg
    46861 atgccccaac gaaacataag cgtctccttt accatagtta atgtcaccgc ttgatatgag
    46921 cgtgaaaatc actcaatgcg atatttttag tgtggtgcac atcgcgcctc ccgacaaagt
    46981 tcgggaggac gaattactta gttttcagat catttttaac gcttttcaca ccatctaccg
    47041 ctttggcgat actttcagca cggtcacttt gtgcctgaga atctacggta ccggagagct
    47101 gaaccacgcc atcggtggtt tcaactttca ctttacgtga agggacgata tcgtccgcca
    47161 gcagtttggc tttgatttca ctggtggtgg cggtgtcacc cgcgtagccc ttcaccgagc
    47221 cttctttagc gtcgcgaacg tgcagtttgt cgctgacaga ggtcacgcct tcaacgcctt
    47281 tcgccacttt cactgcctct tcggcctggg cctgactttc aacgaaaccg ctcagggtca
    47341 cgactttttg atcggttttg acagagatat cggtgctctt gatgttgtca tgatccacca
    47401 gcgccgcctt cactttcgcg gtgatggcgc tgtcatccat gaaattaccg actttattca
    47461 tagagctatc gactttttgc cctgcgcttt cattggtagt ctgcgcgttg ttttccgcgt
    47521 aggcagagcc ggtcgcgacg gcagaggtca acattacagc cagcagagtt ttcgaaatct
    47581 tcagtcttgt catagtcatc gatttattcc tgtatgtttg ctcgtaattt gagcctagca
    47641 acacgaggtt gcattgctga atagggagag acttcaccct ctacagaagt caatggtcgc
    47701 catcacaaaa gcgatgggtg atgaataacg accattacag cctctgaatc agttattaat
    47761 atcggtagaa tgacaatcga cggctttaga tactaatatc tacgcattga acggtattta
    47821 acgccatcag aaatgtcatc actttgttaa atatagatca caattttgaa accgctcggg
    47881 atatcagcga aaacataagc aaaagtgaat gttttaagaa cattccgtaa gcggctaata
    47941 aggaagggaa attgacaggg cgcagcggct accgcgccct ggaggcaaga attaatgctc
    48001 gcgggtctgg tggaactgaa cgtccggata acgttcctgt gccaggcgca ggttgaccat
    48061 gctggtagcg atgtaagcga ggttatcgcc gccatcaagc gccagttggc tttcgttctt
    48121 acgcttgaac tcttcgaatt tcttcgcgtc tgcacattct acccagcggg cagtggcaac
    48181 gttgactgac tcatacactg cttcaacgtt gtattcgctc ttcaggcgcg ctaccaccac
    48241 atcaaactgc agcacaccaa ccgcaccaac gatcagatcg ttgttggaga ttggacggaa
    48301 cacctgcacc gcgccctctt cggaaagctg taccagccct ttgagcagct gtttttgctt
    48361 cagcggatct ttcaggcgga tacgacggaa cagttctggt gcgaagttcg gaataccggt
    48421 gaacttcatc atctcaccct gggtaaaggt gtcgccgatc tgaatggtgc cgtggttgtg
    48481 caggccgagg atatcgcccg gatacgcttc ttcaacgtgc gaacggtcac ccgccataaa
    48541 ggtcagcgcg tcggagatca ccacatcttt cgcagtgcgc acctggcgca gtttcatgcc
    48601 tttttcatat ttaccggaca ccacacgcat aaacgccacg cggtcgcggt gtttcggatc
    48661 catgttggcc tgaattttaa atacgaagcc ggtaaattta tcttcgctcg cttctacggt
    48721 acgggtatcg gtctgacgcg gcatcggcgc aggcgcccac tccaccaggc cattcagcat
    48781 atgatcaacg ccgaagttac ccagcgcagt accgaagaat accggagtga tttcgcccgc
    48841 aaggaacagc tctttgtcga actcgttaga cgcgccttta accagttcca gttcgtcacg
    48901 cagctgctgt gccagatctt caccaaccgc agcatcgaga tccgggttat tcagcccttt
    48961 aacaatgcgg acttcctgaa tggtgtgccc tttaccgctc tgatagagat aggtttcatc
    49021 tttataaagg tggtaaacgc ctttaaacag cttgccgcag ccaattggcc aagtgatcgg
    49081 tgcgcagcca attttcagct cgttctcaac ttcatcgagc agttccatcg ggtcgcggat
    49141 atcacggtca agtttgttca taaaggtgag gatcggcgtg tcgcgcagac gggtaacttc
    49201 catcagctta cgggtacgat cttcaacacc ttttgcggcg tcgataacca tcaggcagca
    49261 gtccaccgcc gtcagggtac gataggtatc ttccgagaag tcttcgtgcc ccggggtgtc
    49321 gagcaggtta accaggcaat cgtgatacgg aaactgcatc acagacgtag taatggagat
    49381 cccacgctgc ttttccatct ccatccagtc cgacttagcg tgctggttgg aaccacggcc
    49441 ttttactgta ccggcggtct gaatggcctg tccgaacagc agcaccttct cggtgatggt
    49501 agttttaccg gcgtccgggt gagaaataat ggcaaaagtg cggcgcttcg ccacctcttt
    49561 caaataagga gacaacgtca taattctttc ttcttgagta agcggcatcg cgccgcgctt
    49621 gttgaaaacg aaaaattgcg gctattttac ccatcaacgg gggggaggca atcagtgttt
    49681 acacaggagc tgctccagtt cgtgcaacga agaaacggtc caggtgggcg cgatgccttc
    49741 tggttgctcg cgattgtgcg cattcagcca gcaggtcgca agcccggcgt tgatgccacc
    49801 gagaatatcg gactcggcag tgtcgccaac catcagcacg cgtgaacggt caggattgcc
    49861 cgcctgttcc agcgcataat cgaaaatttt cttattcggt ttggcaacgc caacttcttc
    49921 ggaaatcacc agcaaatcga agtaatcacg caggcccgtc cgttccagac gcacttgctg
    49981 caaggcgcta aaaccgttgg tgatgatgcc gattttagcg ttgctacgaa tggcgttaag
    50041 cagagaaacc gcgcccggca acggcgtaca gatttccgcc atcgcattaa taaaggcatc
    50101 gttaagcaag cctgcttcaa ctttcagccg ttcggcccag ctctcgaaac gcccgtgctg
    50161 aagctgtaat gaagtgatcg cgccgttttg ataatccacc cacagtggct tgttaacggc
    50221 ctgatagtcc tgaaaatctt cagcggtaaa ggtgacgctg taatcaagaa acatccgctg
    50281 caggccggtg aatgagtcaa aggtaaacag cgtttcatcg gcatcaaaga aaatccagtc
    50341 ccacttcatt attccacctt gtcttacata ctgattggca acgccatgat gatggcgtct
    50401 tcgcgaccgt ccgtggtggg gtagtaattg cggcgaatcg tcgcctcgtt aaagcctaaa
    50461 ctttcgtaca gggcaatggc ggcagcattt gaggcacgga cttccagcca tagtgtcgcc
    50521 acgccgcgtt tttccagttc gtcgatcaga tgttccagca gcgccctacc cagcccctga
    50581 cgctgatagt cgggatcgac cgcaatattg aacaatgtag cttcatccag caccacttgc
    50641 gtaatcgcaa acgccgccat tttgccgttt tgcgttaact gaaagttgag ataacgctcg
    50701 ccctggttgc tggcaaaggt tttttcactc cacggaaagg cgtgggcgcg ttgttcaatg
    50761 tggtaagccg ctggtaaatc agtcgtttcg agggaagaaa tcgtgttcat atgtgcaaat
    50821 ttgttgccat aacgcggcgc gtgccgttgg gtttgcccgt aattcggtga gcgccggtga
    50881 tgccacctga gcgccttcca atgatagcgg ttcgtcagta cccaaccgcc agctgttgca
    50941 gcgactgcct tgcggcagca tcgcgatttt ttctggcgtc agttgcagca cttgatcggg
    51001 gctgacggtt aatgcgcgca gaacatcgct cactaaagga tcagtcaggg cgggaagatc
    51061 gtttgccacc atcaccagac ggacgtgtgc cgggctcgca atggcaatct ccccctgcaa
    51121 cgcgccaggg cgacgcagcg accactgggt aatgcccagt tgctgtaact gccagtctcg
    51181 tcgggatgtc atagcgaatc gctcctgttg tcaggggcgc aaatatagca aattcgtcga
    51241 taccgcgcca acatatggct ataatcgccg ccagtatcaa ttgaggagca ttccatgtct
    51301 gcatttaccc cggcaagtga agtcttgctg cgtcacagtg atgatttcga acaaagccgt
    51361 attctgtttg ccggagactt acaggatgac ctgcccgcgc gtttagatac cgcggccagc
    51421 cgtgctcata cccagcaatt ccaccattgg caggtgttaa gctgccagat gggggataac
    51481 gcccgtttta gtctggtcgc cacggcgaat gatgtcgcag attgcgatac gctgatttac
    51541 tactggccga agaacaaacc ggaagcccag ttccagttga tgaatttact ttctctgctg
    51601 ccagtgggga cagatatttt tgtcgttggc gagaaccgca gcggcgtgcg cagcgctgag
    51661 cagatgctgg cagattatgc gccgttgaat aaagtcgaca gcgctcgtcg ctgtggcctc
    51721 tattttggtc gtctggaaaa acagccagta tttgatgcgg ataaattctg gggcgaatac
    51781 agcgtcgatg gcctgacggt caaaacgctg cctggcgtgt ttagccgcga cggtctggat
    51841 gtcggtagcc agttgctgct ctcgacgtta actccgcaca cgaaaggtaa agtgctggat
    51901 gtcggctgtg gcgcgggtgt actttcagtt gcctttgcgc gccattcgcc gaaaattcgt
    51961 ctcaccttgt gcgatgtctc tgccccggcg gtagaagcca gccgcgcaac acttgcagcc
    52021 aacggtgttg aaggtgaagt ctttgccagc aacgtctttt ccgaggtgaa aggttgtttt
    52081 gatatgatca tctccaaccc gccgttccac gatgggatgc aaaccagcct ggatgcggcg
    52141 caaacgctga ttcgtggcgc ggtgcgtcat cttaatagcg gcggcgagct gcgaattgta
    52201 gcgaacgcct tcctgcctta cccggacgtg ctggatgaga catttggctt ccatgaagtt
    52261 atcgcgcaaa ccgggcgctt caaggtatat cgcgccatta tgacccgcca ggcgaagaaa
    52321 ggttgagtat ctgatatctg tcgccgcata ccgtgtcgga tgcggcgtga acgccttatc
    52381 cgacctacaa aacctgcaca ttagcccttc gtaggccaga taagacgcgc cagcgtcgca
    52441 tctggcatct gcccccgcat gcctaccaaa cctgcacgct agtttcctga tggacatttt
    52501 tccagcaatt acacatcagg cgataattaa ctattgacga aaagctgaaa accactagaa
    52561 tgcgcctccg tggtagcaat tctttttaag aattgatggt atgcgaaggt ggcggaattg
    52621 gtagacgcgc tagcttcagg tgttagtgtc cttacggacg tgggggttca agtccccccc
    52681 ctcgcaccaa aaatcacgtt gatattgctc gcactgggcg aaggtggcgg aattggtaga
    52741 cgcgctagct tcaggtgtta gtgttcttac ggacgtgggg gttcaagtcc cccccctcgc
    52801 accaaacgag gcgatatcaa aaaaagtaag atgactgtgc gaaggtggcg gaattggtag
    52861 acgcgctagc ttcaggtgtt agtgtcctta cggacgtggg ggttcaagtc ccccccctcg
    52921 caccaattat ctttacttcc tttcttgttt cttccctgtt ttgtcatccc tgattcaaat
    52981 tcattatcat cagcatgatg ccagcaataa gcgccaggca cgatggtagc aaaatgtaat
    53041 gccgcaactg tttgtgatac agcatcaccg gcgtcaatac taatcccatc agaatgacaa
    53101 tccgccactg atccggcgat aaatccgcat acatcagccc tgcaatcagc aatcccggca
    53161 gcaacactcc ccagccactg cccagcgtac gttgcaacat ggtttcatct ctttcattga
    53221 taatgaaaac caatatcata tgataatttt tatcatttgc aagccagaga aatcccttgc
    53281 tatcgagtaa acctatcgct aggattagca atcattatca tttagattac tatcacgatt
    53341 atggcctatc gttccgcacc gctctatgaa gatgtcatct ggcgaacgca tctccagccg
    53401 caggatgctg ggcttgcaca agccgtacgg gcgatgatcg ccaaacatcg tgaacatttg
    53461 ctggagttta tccgcctgga tgaacccgct ccgcttaacg ccatgacgct ggcgcaatgg
    53521 tcatcaccga atgcgctaag ttctctactg gcggtctatt ccgatcatat ctatcgcaac
    53581 caaccaacga tgatccgcga gaacaaaccg ctgatctcac tatgggcaca atggtatatc
    53641 ggcctgatgg tgccaccatt aatgctggcg ctactgacgc aggaaaaagc attagatgtg
    53701 tcgccggaac atttccatgc tgagtttcac gaaaccggac gcgtcgcctg tttctgggtc
    53761 gatgtgtgtg aagataaaaa cgcaacacca cattcgccgc agcagcgaat ggaaacgtta
    53821 atcagccagg cgctggttcc ggttgtgcaa gcactagaag cgaccggaga gatcaacggc
    53881 aaacttatct ggagtaatac cggttatttg atcaactggt atctcactga gatgaagcaa
    53941 ctgctcggcg aggctactgt tgaatcgctg cgccatgccc tcttttttga gaaaacgctc
    54001 acgaacggtg aagataatcc actctggcgt accgtggtac tgcgcgacgg cctgctggtg
    54061 cgccgcactt gctgccagcg ttatcgctta ccggatgtgc agcaatgtgg ctattgtacg
    54121 ctgaaatgaa agcactgccg gggaagtaaa cccggcatca tgcggattaa tagggatgca
    54181 acacattact tgcttcgcaa tgccgcatac ataacagaat atcttcggcc tccaacaacc
    54241 ctgcatcaga actgacgccc agtttcgaca tcacattaaa tttatgcgct cggatcgttt
    54301 tgatattacg tttaagctgc tcggcaattt gtgtcattga gtagccacgc gacataaagc
    54361 gcaatatttc tctctccgtc gggcttaacg tccggctttg gttaatgtac cactgattgt
    54421 tcagtcggtc ggtcgcctga cgtacaccat ttaatgacaa aaagagttcc tgatgaaaaa
    54481 tctccagcgt tgacgcttta cttaatacac cgtccagcgg tgatggcgac aatgaaccaa
    54541 tcagccgagc ttcaatatca tcatccacaa taactaaacg ccgggtacgc ggaaacttaa
    54601 tcgccagctc agtcaggcaa gataatccct ctctgcgctc acttctcatg gcagaaaaag
    54661 aaaaaatgac cgccgcaaac gagatccgcg acatggcatc ctgaaatgac tcctgactgg
    54721 tataggtatg gagcttataa gggttgcccg caaactttct gaataaactc tcaataccga
    54781 tactactcat gacgcatttt tctatgagtg cgacatttct cttcttaatt cggctgtgtt
    54841 ccattctgcg gcatctccat aaacagaagg caagttaagt tctcttgcac cctgcgcgct
    54901 atttatccat gcatacattt cagcgttgct gtgaattgcc agtcgacgca tcgcactgtt
    54961 tttttgggcg ctgatagttt tattactttt cttaagcaat gaagctatct ggttgatgcc
    55021 ccatccttta cctaatagcc gcagtacctt gcgctctgaa agagtgagaa tgacggagcg
    55081 atagctaaaa tcatgagttt cagtcattgc cggagaggtc agcatggcgc tgatacgctc
    55141 tgcgtgcgta ttgccggaac gtacggtctt gaccagattc tcaatgggtt caacatcaga
    55201 caataacgtc gccgtagggc acatgagcag ttctacagcc tgggaatacc aggagcgtga
    55261 caccatgaaa acccagtgaa tttcccgata ttgtgagatt aaagaataat aatgttcaca
    55321 aacagaacgt ggatcttcac tttcaccggc taaatcagca atgactaat
//
LOCUS       DAEBXN010000014        55334 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.014, whole genome shotgun sequence.
ACCESSION   DAEBXN010000014 DAEBXN010000000
VERSION     DAEBXN010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55334
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.014"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(111..1775)
                     /gene="glnS"
                     /locus_tag="KJL99_000827"
     CDS             complement(111..1775)
                     /gene="glnS"
                     /locus_tag="KJL99_000827"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HBD4435668.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHITVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(1978..3924)
                     /gene="nagE"
                     /locus_tag="KJL99_000828"
     CDS             complement(1978..3924)
                     /gene="nagE"
                     /locus_tag="KJL99_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HBD4435669.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLLIKAQGHVVAGQTPLYEIKK"
     gene            4257..5057
                     /gene="nagB"
                     /locus_tag="KJL99_000829"
     CDS             4257..5057
                     /gene="nagB"
                     /locus_tag="KJL99_000829"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBD4435670.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMIMVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5117..6265
                     /gene="nagA"
                     /locus_tag="KJL99_000830"
     CDS             5117..6265
                     /gene="nagA"
                     /locus_tag="KJL99_000830"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBD4435671.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIDVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            6274..7494
                     /gene="nagC"
                     /locus_tag="KJL99_000831"
                     /gene_synonym="nagR"
     CDS             6274..7494
                     /gene="nagC"
                     /locus_tag="KJL99_000831"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HBD4435672.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7542..8294
                     /gene="nagD"
                     /locus_tag="KJL99_000832"
                     /pseudo
     CDS             7542..8294
                     /gene="nagD"
                     /locus_tag="KJL99_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
     gene            8691..10355
                     /gene="asnB"
                     /locus_tag="KJL99_000833"
     CDS             8691..10355
                     /gene="asnB"
                     /locus_tag="KJL99_000833"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HBD4435673.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKNHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNVKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            10734..10810
                     /locus_tag="KJL99_000834"
     tRNA            10734..10810
                     /locus_tag="KJL99_000834"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10768..10770,aa:Met,seq:cat)
     gene            10819..10903
                     /locus_tag="KJL99_000835"
     tRNA            10819..10903
                     /locus_tag="KJL99_000835"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10853..10855,aa:Leu,seq:tag)
     gene            10927..11001
                     /locus_tag="KJL99_000836"
     tRNA            10927..11001
                     /locus_tag="KJL99_000836"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10959..10961,aa:Gln,seq:ttg)
     gene            11036..11110
                     /locus_tag="KJL99_000837"
     tRNA            11036..11110
                     /locus_tag="KJL99_000837"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11068..11070,aa:Gln,seq:ttg)
     gene            11126..11202
                     /locus_tag="KJL99_000838"
     tRNA            11126..11202
                     /locus_tag="KJL99_000838"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11160..11162,aa:Met,seq:cat)
     gene            11251..11325
                     /locus_tag="KJL99_000839"
     tRNA            11251..11325
                     /locus_tag="KJL99_000839"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11283..11285,aa:Gln,seq:ctg)
     gene            11363..11437
                     /locus_tag="KJL99_000840"
     tRNA            11363..11437
                     /locus_tag="KJL99_000840"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11395..11397,aa:Gln,seq:ctg)
     gene            complement(11591..12766)
                     /gene="ubiF"
                     /locus_tag="KJL99_000841"
     CDS             complement(11591..12766)
                     /gene="ubiF"
                     /locus_tag="KJL99_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HBD4435674.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPTRIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            12912..14336
                     /gene="miaB"
                     /locus_tag="KJL99_000842"
     CDS             12912..14336
                     /gene="miaB"
                     /locus_tag="KJL99_000842"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HBD4435675.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDASHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            14489..15529
                     /locus_tag="KJL99_000843"
     CDS             14489..15529
                     /locus_tag="KJL99_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HBD4435676.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            15526..15993
                     /gene="ybeY"
                     /locus_tag="KJL99_000844"
     CDS             15526..15993
                     /gene="ybeY"
                     /locus_tag="KJL99_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HBD4435677.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            16083..16961
                     /gene="corC"
                     /locus_tag="KJL99_000845"
     CDS             16083..16961
                     /gene="corC"
                     /locus_tag="KJL99_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HBD4435678.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            16986..18524
                     /gene="lnt"
                     /locus_tag="KJL99_000846"
     CDS             16986..18524
                     /gene="lnt"
                     /locus_tag="KJL99_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HBD4435679.1"
                     /translation="MDFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIATPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     AEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFAREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            18921..19829
                     /gene="gltI"
                     /locus_tag="KJL99_000847"
     CDS             18921..19829
                     /gene="gltI"
                     /locus_tag="KJL99_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HBD4435680.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            19999..20739
                     /gene="gltJ"
                     /locus_tag="KJL99_000848"
     CDS             19999..20739
                     /gene="gltJ"
                     /locus_tag="KJL99_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HBD4435681.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWVWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQTYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            20739..21413
                     /gene="gltK"
                     /locus_tag="KJL99_000849"
     CDS             20739..21413
                     /gene="gltK"
                     /locus_tag="KJL99_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HBD4435682.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            21413..22138
                     /gene="gltL"
                     /locus_tag="KJL99_000850"
     CDS             21413..22138
                     /gene="gltL"
                     /locus_tag="KJL99_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HBD4435683.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMAPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            22256..23191
                     /gene="rihA"
                     /locus_tag="KJL99_000851"
     CDS             22256..23191
                     /gene="rihA"
                     /locus_tag="KJL99_000851"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HBD4435684.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVIVDVDRQGFVDLLADRLKFYA"
     gene            23275..24945
                     /gene="hscC"
                     /locus_tag="KJL99_000852"
                     /pseudo
     CDS             23275..24945
                     /gene="hscC"
                     /locus_tag="KJL99_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
     gene            complement(25005..26456)
                     /gene="djlC"
                     /locus_tag="KJL99_000853"
     CDS             complement(25005..26456)
                     /gene="djlC"
                     /locus_tag="KJL99_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HBD4435685.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMVELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLGA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(26453..27160)
                     /locus_tag="KJL99_000854"
     CDS             complement(26453..27160)
                     /locus_tag="KJL99_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HBD4435686.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRAGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            27262..27816
                     /locus_tag="KJL99_000855"
     CDS             27262..27816
                     /locus_tag="KJL99_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HBD4435687.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTMLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(27826..29253)
                     /locus_tag="KJL99_000856"
     CDS             complement(27826..29253)
                     /locus_tag="KJL99_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HBD4435688.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCY
                     YWREYPSTQADALMLQWCRQHRVYYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(29250..29957)
                     /locus_tag="KJL99_000857"
     CDS             complement(29250..29957)
                     /locus_tag="KJL99_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HBD4435689.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRIIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            30121..31098
                     /locus_tag="KJL99_000858"
     CDS             30121..31098
                     /locus_tag="KJL99_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HBD4435690.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVTWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFWYK
                     QAALQGHSYAQDNLADLYKDGEGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEALL"
     gene            complement(31168..31650)
                     /locus_tag="KJL99_000859"
     CDS             complement(31168..31650)
                     /locus_tag="KJL99_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HBD4435691.1"
                     /translation="MNKVAQYYRELVASLNERLRNGERDIDALVEQARERVIKTGELT
                     RTEIDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EIFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGYDQFQRRPFEP
                     "
     gene            31885..34467
                     /gene="leuS"
                     /locus_tag="KJL99_000860"
     CDS             31885..34467
                     /gene="leuS"
                     /locus_tag="KJL99_000860"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HBD4435692.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALQAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            34482..35063
                     /gene="lptE"
                     /locus_tag="KJL99_000861"
     CDS             34482..35063
                     /gene="lptE"
                     /locus_tag="KJL99_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HBD4435693.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTMLGN"
     gene            35063..36094
                     /gene="holA"
                     /locus_tag="KJL99_000862"
     CDS             35063..36094
                     /gene="holA"
                     /locus_tag="KJL99_000862"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HBD4435694.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            36096..36737
                     /gene="nadD"
                     /locus_tag="KJL99_000863"
     CDS             36096..36737
                     /gene="nadD"
                     /locus_tag="KJL99_000863"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HBD4435695.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            36761..37372
                     /locus_tag="KJL99_000864"
     CDS             36761..37372
                     /locus_tag="KJL99_000864"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HBD4435696.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            37632..37949
                     /gene="rsfS"
                     /locus_tag="KJL99_000865"
                     /gene_synonym="rsfA"
     CDS             37632..37949
                     /gene="rsfS"
                     /locus_tag="KJL99_000865"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HBD4435697.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            37953..38420
                     /gene="rlmH"
                     /locus_tag="KJL99_000866"
     CDS             37953..38420
                     /gene="rlmH"
                     /locus_tag="KJL99_000866"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HBD4435698.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            38451..40352
                     /gene="mrdA"
                     /locus_tag="KJL99_000867"
     CDS             38451..40352
                     /gene="mrdA"
                     /locus_tag="KJL99_000867"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HBD4435699.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     EPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTANKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            40355..41467
                     /gene="mrdB"
                     /locus_tag="KJL99_000868"
                     /gene_synonym="rodA"
     CDS             40355..41467
                     /gene="mrdB"
                     /locus_tag="KJL99_000868"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HBD4435700.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            41478..42566
                     /gene="rlpA"
                     /locus_tag="KJL99_000869"
     CDS             41478..42566
                     /gene="rlpA"
                     /locus_tag="KJL99_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HBD4435701.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTKLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTASSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            42705..43916
                     /gene="dacA"
                     /locus_tag="KJL99_000870"
     CDS             42705..43916
                     /gene="dacA"
                     /locus_tag="KJL99_000870"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HBD4435702.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFSDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            44027..44290
                     /locus_tag="KJL99_000871"
     CDS             44027..44290
                     /locus_tag="KJL99_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HBD4435703.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            44391..45032
                     /gene="lipB"
                     /locus_tag="KJL99_000872"
     CDS             44391..45032
                     /gene="lipB"
                     /locus_tag="KJL99_000872"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HBD4435704.1"
                     /translation="MYQDKILVRQLGLQPYEPISRAMHEFTDTRDNSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            45291..46244
                     /locus_tag="KJL99_000873"
     CDS             45291..46244
                     /locus_tag="KJL99_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HBD4435705.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICRKGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFILRDPILHGVIDVIRNAF"
     gene            46453..47418
                     /gene="lipA"
                     /locus_tag="KJL99_000874"
     CDS             46453..47418
                     /gene="lipA"
                     /locus_tag="KJL99_000874"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HBD4435706.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(47519..47722)
                     /gene="tatE"
                     /locus_tag="KJL99_000875"
     CDS             complement(47519..47722)
                     /gene="tatE"
                     /locus_tag="KJL99_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HBD4435707.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(47851..48639)
                     /locus_tag="KJL99_000876"
     CDS             complement(47851..48639)
                     /locus_tag="KJL99_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HBD4435708.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHLPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPILNNRRFAPPQLL"
     gene            48732..49115
                     /gene="crcB"
                     /locus_tag="KJL99_000877"
     CDS             48732..49115
                     /gene="crcB"
                     /locus_tag="KJL99_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HBD4435709.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTVH"
     gene            complement(49169..49378)
                     /gene="cspE"
                     /locus_tag="KJL99_000878"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(49169..49378)
                     /gene="cspE"
                     /locus_tag="KJL99_000878"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HBD4435710.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(49553..50113)
                     /gene="pagP"
                     /locus_tag="KJL99_000879"
     CDS             complement(49553..50113)
                     /gene="pagP"
                     /locus_tag="KJL99_000879"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HBD4435711.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADERMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            50702..52087
                     /gene="dcuC"
                     /locus_tag="KJL99_000880"
     CDS             50702..52087
                     /gene="dcuC"
                     /locus_tag="KJL99_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HBD4435712.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(52128..52469)
                     /locus_tag="KJL99_000881"
                     /pseudo
     CDS             complement(52128..52469)
                     /locus_tag="KJL99_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(52807..53316)
                     /locus_tag="KJL99_000882"
     CDS             complement(52807..53316)
                     /locus_tag="KJL99_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBD4435713.1"
                     /translation="MKSANEINQWMTPKQITELDGMPGTIQGVHKRAKKEGWPKRSQE
                     GRRGPGVEYIPAIPAMQKIAIEEMNADTLKMFSLLINKLGENEVREIELNMAKYGLSG
                     LMQERPSISVDTLLATLGIDRQTLQTALALHKLPPETRQEILSMYGVHKQEEPVAPLL
                     EPQDAKKAV"
     gene            53479..53724
                     /locus_tag="KJL99_000883"
     CDS             53479..53724
                     /locus_tag="KJL99_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013024337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBD4435714.1"
                     /translation="MRKMSNDWHRADVIAALKKTGTPLAKLSRRSGYSTGTLANALVR
                     PWPKGERIIADALNMKPEEIWPSRYLRDHNERVDISN"
     gene            53699..55330
                     /locus_tag="KJL99_000884"
                     /pseudo
     CDS             53699..55330
                     /locus_tag="KJL99_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683974.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
BASE COUNT        13893 a        13930 c        13843 g        13668 t
ORIGIN      
        1 atgtaggcct gataagcgaa gcgcatcagg caatttagcg tttgaaattt attgtctatt
       61 tattgccgga tgcgacgtaa atgcctcacc cggcttggta gaacttaaaa ttactcgcct
      121 actttcgccc aggtatcacg cagcccaacg gtgcggttaa ataccggttt ttcggccgtg
      181 gaatggcggc tgtcgaggca gaagtaacct tcacgctcaa actggaatgc tttacccgca
      241 accgcatctt tcagcgacgg ttcagcaaag ccctgtttga tcaccagcga ttccgggtta
      301 atcaccgaca ggaaatcatc cgcagcacct gggttaggca cgctgaacag acgatcatac
      361 aaacggattt caaccggcag cgcatgtgcc gcgctcaccc agtgaataac acctttgact
      421 ttacgaccat ctgccggatc tttgcttaag gtgtcggcgt cataagtaca gaagatggtg
      481 gtgatattac cttcggcatc tttctccacg cgttcagcct taatgacgta agcattacgc
      541 agacgcactt ctttacccag caccagacgt ttgtactgct tgttagcttc ttcgcggaaa
      601 tcggcgcgat cgatccaaat ttcaccgcta aacggcacct gacggctccc catttccggt
      661 ttgttcggat ggttcggcat ggtgaccatt tcgccttcgc cctgatagtt ttcgataacc
      721 agtttcaccg gatcgataac cgccattgcg cgcggcgcat tttcgttgag atcttcacgg
      781 atgcaggatt ccagcgacgc catctcaatg gtgttgtcct gcttggtcac gccgatgcgt
      841 ttgcagaact cacgaataga agccgcagtg taaccacgac gacgcagacc ggaaatggtc
      901 ggcatacgcg ggtcatccca gccttcaacg tgcttgtcgg tcaccagcag gttcagctta
      961 cgcttggaca tcacagtgta ttccagattc aggcgcgaga attcgtactg gcgcggatga
     1021 acaggaatgg tgatgttatc cagtacccag tcgtacagac gacggttgtc ctggaactca
     1081 agcgtacaca gagagtgcgt aataccttcc agcgcatcgc tgatgcaatg ggtgaagtcg
     1141 tacatcgggt agatgcacca cttgttgcca gtctggtggt gttcagcaaa tttaatgcgg
     1201 tacagcaccg gatcgcgcat tacgataaat ggcgacgcca tgtcgatttt cgcacgcagg
     1261 caggctttac cttcttcaaa accaccggca cgcatttttt cgaacagcgc caagttctct
     1321 tcaacgctgc ggtcgcggta cgggctgttt ttacccggct gcgtcagggt accgcggtat
     1381 tcgcggatct gttccggtgt cagttcatca acgtacgcca ggcctttatt gatcagttcg
     1441 atcgcatagg cgtggagctg atcaaaataa tcggaggagt aacggacgtt accagaccag
     1501 tgaaaaccta accactctac gtcgtttttg atcgaatcga catactcgat atcttctttt
     1561 accggattag tgtcgtcgaa acgcaggttg cactggcctt tatagtcctg ggcgatcccg
     1621 aagttcaggc agatagattt cgcatggcca atatgcagat agccattcgg ctccggcggg
     1681 aaacgggtgt gtactgtgat gtgcttacca ctggccagat cttcatcgat gatctgacgg
     1741 ataaagttag tcgggcgggc ttctgcctca ctcatcgtgg attcctcaaa gcgtaaacaa
     1801 cgtataacgg cgtatgatct tataagcggg acaggctgac aactgttagt tttttaaaat
     1861 tgataatcta acaaggatta tggggatgat tgatgaaaaa aaaagcggca gagaagatct
     1921 ccaccgcttg tcattgttgg atgcgacgct gccgtgtcgc atcaggcata aagcagatta
     1981 ctttttgatt tcatacagcg gtgtttgacc cgccacaaca tggccctgag ctttaatgag
     2041 caagccactg aaatcgtcga tattgctgca aaccaccggg ctaatcatcg agcgggcgtt
     2101 agcgttcagg taatccagat ccatttccag aatcggttgc cctgcgctta cctgcgcacc
     2161 ctcttccacc agacgtttaa agcctttacc ttccagcgct acggtgtcga tacccatatg
     2221 gacgacgatc tccgcgcctt tttcggtttc caggcagaac gcgtggttgg tgttgaagat
     2281 tttcacgatt gtccctgcgg ctggtgatac gacgatttta tctgtcggtt tcaccgccac
     2341 accgtcaccc accgctttgc tggcgaatgc ttcgtcagga acctgatcca gtgccacgac
     2401 atcaccggta atcggcgata ccagctccgc gatagatacc gcgtttggta cagcctgcgg
     2461 ttttgctaca ggcgcggcag ttgccggagt tgcttcagct gacgcagcgg ctaccggacc
     2521 acgggcaacg actttcttca tcgcatcgcc gatggattct gctttcgcgc caacaatcac
     2581 ctgaatagtc tgtttgttca gtttcactac accagaagca cccagacgtt tacacatcgt
     2641 atcgttgacg cgggcagagt cagccacggt caggcgcaga cgggtaatac aggcatcaat
     2701 cgctttcagg ttgtcagtac cgccaaccgc agcaatatag ttggttgcca gttgagtcag
     2761 accttcttca gtattgctgt tggcttcttc agtaacgatc tcgtcttctt tatcttcacg
     2821 acccggcgtt ttcaggttga acatgcggat aaccaaactg aacaccacga agtagatagc
     2881 gaagaagata acgcccatca ccagcagcat ccagacgttc tggctggcgg caggcaggtt
     2941 atacatcaac gcgtagtcga tagcacccgc agagaaagag aagccggcat ggatacccag
     3001 cagcgttgcc acaaacaggc tgataccggt cagcagtgcg tgcaggaggt acagcagcgg
     3061 cgcaaggaac atgaacagga attccagcgg ctcagtcaca ccggtcagga acgcagtaac
     3121 agcaacagaa agcagcatcc cgccaaccat cggacgacgc tctttcggtg ctgcgaagta
     3181 catcgccagc gccgcacccg gcagaccgaa catcatgatc gggaagaagc cggacatgaa
     3241 catccccgcg gtgccgtcac cagcgtagaa acggttgatg tcgccgtgga aaaccgtacc
     3301 cgccgcgttg gtgaattcac caatctggaa ccaggcgatg gtgttcagca cctgatgcag
     3361 accggttggg atcagcagac ggttgatgaa accaaagata ccggaaccca gcgcgcccgc
     3421 agaaacgatc cactcgccgc ctgcatggat agcgtgctgt accggcggcc agacgtaacc
     3481 aaaaatggcc gccagcacca gacagaagaa gccggtggcg atcggcacaa agcgtttgcc
     3541 gccgaagaag ctcaggaagt ccggcagttt aatatcggac caacggttat aggctgcgcc
     3601 accaaccaga ccggtaatga tacccgccag tacacccatg ttaatttctg ggttgatggt
     3661 caccatcgct ttggttaaca caaagtaacc taccgcaccc gccagcgccg ccgcacctgc
     3721 gctgtctttc gaccagctgg atgccacacc gatggcgaag attaatgcga ggttatcaaa
     3781 aattgcaccg cccgcctggg caataaacgc aacgttaagt aaatctggct gaccgaatcg
     3841 cagcaacagc gccgccaccg gcagcaccgc gatagggagc tgtaacgccc taccgagtcg
     3901 ctggaaaaaa cctaaaatat tcatcttatt ccccctacga gaaccctatt tggctcgttt
     3961 aaagccgtat ttttattttg ctgcaaattt tactgccgat gttctgtaat cagattgtta
     4021 gatcatctgc tacagagtgt gtgaaaattt aattcgcatc gcaaattaaa cgcgtgtctt
     4081 ttgtgagttt tgtcaccaaa tatcgttatc atcactccct tttactggct aaaccagaaa
     4141 acttatttta tcattcaaaa aatcaagtcg gattgacgcc tgtctgcgca aatccaggtt
     4201 acgcttaaag atgtctaatc cgccaacggc ttacatttta cttattgagg tgaataatga
     4261 gactgatccc cctgactacc gctgaacagg tcggcaaatg ggctgctcgc catatcgtca
     4321 atcgtatcaa tgcgttcaaa ccaactgccg atcgtccgtt tgtactgggc ctgccgactg
     4381 gcggcacgcc gatgaccacc tataaagcgt tagtcgaaat gcataaagca ggccaggtca
     4441 gctttaagca cgttgtcacc ttcaacatgg acgaatatgt cggtctgccg aaagagcatc
     4501 cggaaagcta ctacagcttt atgcaccgta atttcttcga tcacgttgat attccagcag
     4561 aaaacatcaa ccttctcaac ggcaatgccc cggatatcga tgccgagtgc cgccagtatg
     4621 aagaaaaaat ccgttcttac ggaaaaattc atctgtttat gggcggtgta ggtaacgacg
     4681 gtcatattgc atttaacgaa ccggcgtctt ctctggcttc tcgtactcgt atcaaaaccc
     4741 tgactcatga cactcgcgtc gcgaactctc gtttctttga taacgatgtc aatcaggtgc
     4801 caaaatatgc cctgactgtc ggtgttggta cactgctgga tgccgaagaa gtgatgatta
     4861 tggtgctggg tagccagaaa gcactggcgc tgcaggccgc cgttgaaggt tgcgtgaacc
     4921 atatgtggac catcagctgt ctgcaactgc atccgaaagc gatcatggtg tgcgatgaac
     4981 cttccaccat ggagctgaaa gttaagactt taagatattt caatgaatta gaagcagaaa
     5041 atatcaaagg tctgtaattg ttatccctgc cctggttcct tgctcagggc aatatttttt
     5101 aaaatcgggg ggcagaatgt atgcattaac ccagggccgg atctttaccg gccacgaatt
     5161 tcttgatgac cacgcggttg ttatcgctga tggcctgatt aaaagcgtct gtccggtagc
     5221 ggaactgccg ccagagatcg aacaacgttc actgaacggg gccattctct cccccggttt
     5281 tatcgatgtg cagttaaacg gctgcggcgg cgtacagttt aacgacaccg ctgaagcggt
     5341 cagcgtggaa acgctggaaa tcatgcagaa agccaatgag aaatcaggct gtactaacta
     5401 tctgccgacg cttatcacca ccagcgatga gctgatgaaa cagggcgtgc gcgttatgcg
     5461 tgagtacctg gcaaaacatc cgaatcaggc gttaggtctg catctggaag gtccgtggct
     5521 gaatctggta aaaaaaggca cccataatcc gaattttgtg cgtaagcctg atgccgcgct
     5581 ggtcgatttc ctgtgtgaaa acgccgacgt cattaccaaa gtgaccctgg caccggaaat
     5641 ggttcctgcg gaagtcatca gcaaactggc aaatgccggg attgtggttt ctgccggtca
     5701 ctccaacgcg acgttgaaag aagcaaaagc cggtttccgc gcggggatta cctttgccac
     5761 ccatctgtac aacgcgatgc cgtatattac cggtcgtgaa ccgggcctgg cgggcgcgat
     5821 cctcgacgaa gctgacattt attgcggtat tatcgctgat ggcctgcatg ttgattacgc
     5881 caacattcgt aacgctaaac gccttaaagg cgacaaactg tgtctggtta ccgacgccac
     5941 cgcgccagca ggtgccaaca ttgaacagtt catttttgcg ggtaaaacaa tatactaccg
     6001 taacggactt tgtgtggatg agaacggtac gttaagcggt tcatccttaa ccatgattga
     6061 aggcgtgcgt aatctggtcg aacattgcgg tatcgcactg gatgaagtgc tgcgtatggc
     6121 gacgctctat ccggcgcgtg cgattgacgt tgaaaaacgt ctcggcacgc tcgccgcagg
     6181 taaagtagcc aacctgaccg cattcacacc tgattttaaa atcaccaaga ccatcgttaa
     6241 cggtaacgag gtcgtaactc aataagagaa agtatgacac caggcggaca agctcagata
     6301 ggtaatgttg atctcgtaaa acagcttaac agcgcggcgg tttatcgcct gattgaccag
     6361 tacgggccaa tctcgcggat tcagattgcc gagcaaagcc agcttgcccc cgccagcgta
     6421 accaaaatta cgcgtcagct tatcgaacgc gggctgatca aagaagttga tcagcaggcc
     6481 tccaccgggg gccgccgcgc tatctccatc gtcaccgaaa cccgcaattt ccacgcaatc
     6541 ggcgtacggc ttggtcgtca tgacgccacc atcactctgt ttgatctcag cagcaaagtg
     6601 ctggcagaag aacattaccc gctgccggaa cgtacccagc agacgctgga acatgccctg
     6661 ctgaatgcca ttgctcagtt tattgatagc taccagcgca agctgcgcga gctaatcgcc
     6721 atttcggtga tcctgccagg gcttgttgac ccggacagcg gcaaaattca ttacatgccg
     6781 catattcagg tagaaaactg ggggctggta gaagcactgg aagaacgttt taaagtgacc
     6841 tgtttcgttg gtcacgatat ccgtagtctg gcgctggcgg agcactactt cggtgcaagt
     6901 caggattgcg aagactccat tctggtgcgt gtccatcgcg gaaccggggc cgggattatc
     6961 tctaacgggc gcatttttat tggccgcaac ggtaacgtcg gtgaaattgg ccatattcag
     7021 gtcgaaccgc tgggtgaacg ctgccactgc ggcaactttg gctgcctgga aactatcgcc
     7081 gccaacgctg ccattgaaca acgggtgttg aatctgttaa agcagggcta ccagagccgt
     7141 gtgccactgg acgactgcac catcaaaact atctgcaaag ccgcgaacaa aggcgatagc
     7201 ctggcctcgg aagtgattga gtatgtcggt cgtcatctgg gtaaaaccat cgccattgct
     7261 atcaacttat ttaatccgca aaaaattgtt attgctggtg aaatcaccga agccgataaa
     7321 gtgctgctcc ctgctattga aagctgcatt aatacccaag cgctgaaggc gtttcgtact
     7381 aatctgccgg tggtacgttc tgagctggat caccgctcgg caatcggcgc ttttgcgctg
     7441 gtaaaacgcg ccatgctcaa cggtattttg ctccagcatt tgctggaaaa ttaatgtgct
     7501 tttatagtgg cgcttattgt tgtcaatatt ctgggtagtc catgaccatt aaaaatgtaa
     7561 tttgcgatat cgacggcgtg ctgatgcacg ataacgtcgc cgtaccgggt gcagcggaat
     7621 ttttgcacgg gattatggat aaaggcctgc cgctggtgtt gctgaccaac tatccttcgc
     7681 agactgggca agatctggcg aaccgctttg ccactgccgg tgtcgatgtg ccggatagcg
     7741 tgttttatac ctctgcgatg gcgaccgccg atttcctgcg tcgctaggaa ggcaagaaag
     7801 cgtatgtggt gggcgaaggc gcactgattc atgaactgta caaagccggt ttcactatta
     7861 ccgatgtgaa ccctgatttc gtgattgttg gcgaaacacg ttcctacaac tgggacatga
     7921 ttcataaagc agcctatttc gtcgctaacg gtgcacgctt tatcgccacc aacccggaca
     7981 cccacgggcg cggtttttat cccgcttgtg gcgcgttgtg tgcagggatt gagaaaatct
     8041 ccgggcgcaa accgttctat gttggtaagc ccagcccgtg gatcatccgc gcggcattaa
     8101 acaaaatgca ggcgcattcg gaagaaacag tgattgtcgg cgataacctg cgtaccgata
     8161 ttctggcggg cttccaggcc ggtctggaga cgattctggt gctttctggt gtttcatcgc
     8221 tcgacgatat cgacagtatg ccgttccgcc caagctggat ttacccgtca gtcgctgaaa
     8281 tcgacgttat ctgaatacca cccacgtctg cggacgtggt tggctctgat gacaaacgcc
     8341 aaactgcctg atgcgctacg cttatcaggc ctacgcagct cctgcaatat attgaatttt
     8401 catgcttttg taggccggat aaggcgttca cgccgcatcc ggcatgaaca aatcgcactt
     8461 tatcagcaat ttggcccccg taaaatataa ccattcaata aaacccgcta aaaattatcg
     8521 attcatcaat aaatacatcc tcacaccaga ccctttttca ccaatcgaca atcaccatta
     8581 cgttttttat ttttgcactg ccaaatagct tgcgcacagt gctgctttgc ggcatttttt
     8641 taaacaagca aacacaacaa gcaacaaata ccaggttaac ggagaaggtt atgtgttcaa
     8701 tttttggcgt attcgatatc aaaacagacg cagttgagct gcgtaagaaa gcactcgagc
     8761 tgtcacgcct gatgcgtcat cgtggcccgg actggtccgg tatttatgcc agcgataacg
     8821 ccattctcgc tcacgaacgt ctgtcaattg ttgacgttaa cgcgggggcg caacctctct
     8881 acaaccaaca aaaaaaccac gtactggcgg taaacggtga aatctacaac caccaggcat
     8941 tgcgcgctga atatggcgat cgttatcagt tccagacagg gtctgactgt gaagtgatcc
     9001 tcgcgctgta tcaggaaaaa gggccggaat ttctcgacga cttgcagggc atgtttgcct
     9061 ttgccctgta cgacagcgaa aaagatgcct acctgattgg tcgcgaccat ctggggatca
     9121 tcccactgta tatggggtat gacgaacacg gtcagctgta tgtggcctca gaaatgaaat
     9181 cgctggtgcc agtttgccgc acgattaaag agttcccggc ggggagctat ttgtggagtc
     9241 aggacggcga aatccgttct tattatcatc gcgactggtt cgactttgat gcagtgaaag
     9301 ataacgtgac cgacaaaaac gagctgcgtc aggcactgga agattccgtt aaaagccatc
     9361 tgatgtctga tgtgccttac ggtgtgctac tttctggtgg tctggattcc tcaattattt
     9421 ccgctatcac caagaaatac gcagcccgtc gcgtggaaga tcaggaacgc tctgaagcct
     9481 ggtggccgca gttacactcc tttgctgtag gtctgccggg ttcaccggat cttaaagcag
     9541 cccaggaagt ggcaaaccat ctgggcacgg tgcatcacga aattcacttc actgtacagg
     9601 aaggtctgga tgccatccgc gacgtgattt accacatcga aacttatgat gtgaccacta
     9661 ttcgcgcttc aacaccgatg tatttaatgt cgcgtaagat caaggcgatg ggcattaaaa
     9721 tggtgctgtc cggtgaaggt tctgatgaag tgtttggcgg ttatctttac ttccataaag
     9781 cgcccaacgt taaagaactg catgaagaga cggtgcgtaa actgctggcc ttgcatatgt
     9841 atgactgcgc gcgcgccaac aaagcgatgt cagcctgggg cgtggaagca cgcgttccgt
     9901 tcctcgacaa aaaattcctt gatgtggcga tgcgcattaa cccgcaggat aaaatgtgcg
     9961 gtaacggcaa aatggaaaaa cacatcctgc gtgaatgttt tgagtcatac ctgcccgcaa
    10021 gcgtggcctg gcggcagaaa gagcagttct ccgatggcgt cggttacagt tggatcgaca
    10081 ccctgaaaga agtggcggcg cagcaggttt ctgatcagca actggaaact gcccgcttcc
    10141 gcttcccgta caacacgccg acctcaaaag aagcgtatct gtaccgcgag atctttgaag
    10201 aactgttccc acttccgagt gccgctgagt gcgtgccggg cggtccttcc gtcgcgtgtt
    10261 cttcagctaa agcgatcgaa tgggatgaag cgttcaaaaa aatggacgat ccgtctggtc
    10321 gtgcggttgg cgttcaccag tcggcgtata agtaagaaat gcggtactgc cccggagaaa
    10381 tccggggctg ttttagttat tgcgctgctt gttgattctg atatgcagta aggaaatgtt
    10441 ccgggtttgc cacatcactg tttgacaact catcactgta ggccagtatt tcatagatta
    10501 atttcccacc actattgttg aattccgaat ttcatcctgt acgacaaaag aatggctgta
    10561 gggtggggaa aaacacaaag taaacaataa ttgacgaata tagcgccacg ctgttcgcaa
    10621 cctaaccaaa cagtcacttt cgagcaattt tccttgaaaa agaggttgac gctgcaaggc
    10681 tctatacgca taatgcgccc cgcaacgccg ataaggtatc gcgaaaaaaa gatggctacg
    10741 tagctcagtt ggttagagca catcactcat aatgatgggg tcacaggttc gaatcccgtc
    10801 gtagccacca tcttttttgc gggagtggcg aaattggtag acgcaccaga tttaggttct
    10861 ggcgccgcaa ggtgtgcgag ttcaagtctc gcctcccgca ccattcacca gaaagcgttg
    10921 tacggatggg gtatcgccaa gcggtaaggc accggttttt gataccggca ttccctggtt
    10981 cgaatccagg taccccagcc atcttcttcg agtaagcggt tcaccgcccg gttattgggg
    11041 tatcgccaag cggtaaggca ccggtttttg ataccggcat tccctggttc gaatccaggt
    11101 accccagcca tcgaagaaat aatctggcta cgtagctcag ttggttagag cacatcactc
    11161 ataatgatgg ggtcacaggt tcgaatcccg tcgtagccac caaattctga atgtgtcgaa
    11221 tacgttcggc aaattcaaaa accaatttgt tggggtatcg ccaagcggta aggcaccgga
    11281 ttctgattcc ggcattccga ggttcgaatc ctcgtacccc agccaattta ttcaagacgc
    11341 ttacatcgta agtgcaccca gttggggtat cgccaagcgg taaggcaccg gattctgatt
    11401 ccggcattcc gaggttcgaa tcctcgtacc ccagccacat taaaaaagct cgcttcggcg
    11461 agctttttgc ttttctgcgt tcattcaatg tcgaatgcga tgttgacacg tcttatcctt
    11521 caatgtcgga tgcgacgctg ccgcgtctta tccgacctac ggctggcacg catccggcaa
    11581 tgttgtaagg ctacaaccct aacgcatatt tcagcgcctg acgtttcaac acgccagcac
    11641 gctctgccgc cattaacccg agattacgca caaaacgcag cggcggcaga ttattactga
    11701 atccggcata aaacagatcc ataccacttt gcataatgaa gttatccgcc atgcgccgca
    11761 tctggtaacg cttgaggaca ggataactgg cccacgcttc accgtagctg cgggcattca
    11821 ccagaacatc aatcagggca tcgacatcac gataaccaag attcaccccc tgccccgcca
    11881 gcggatggat ggtatgcgcg gcatcgccca ccagcgcaag ccccggctgc acatactgca
    11941 acgcatgtcg gcgcgtcagc ggaaacgcac cagcggcaag cggtgtaacg taacccagac
    12001 gcgacgggaa atgcttcgcg atttccgcct ggagttgcgc catattcata ttctgcaact
    12061 ggcgaatacg cgtcggagag tcataccaca ccagcgatgc ccagttatca aacaacggca
    12121 gaaacgcacg cggtccgtcc ggggtaaatt gctgccaggt gctgtcgccg ggatcgttct
    12181 cacactgtac gctaatcaac atgcacgact gcgcatactg ccaggcatga acgccaattc
    12241 ccgccatctg ccgcacctga gaatttgcgc cgtcggcacc aatcaccagt ttcgcgcgga
    12301 tcgtttcacc gcccttcagt tccagctcct gaagatcatt atctcgatgt aatgcaatca
    12361 gcgaggttgg cacacgtaac gttactttcg gatgcgcttc cagcgcctgc cacaacgcct
    12421 gttgcaggac tgtgttttcc accatgtagc caagcaacgg caactttaat tcggctgcgt
    12481 caaataccac atgtgccgtt tcccactccc aggtttccag tctgcggtaa ggatggcaac
    12541 gcatagcctg tactgcatcc cagaccccta accctttaag caatgatacc gaagccgcgc
    12601 tgatcgccga gatccgcacg tccggttggc tgtcagcgac aaacggtgca ggttctgcgt
    12661 gctcgatcac cgttaccgaa aatccgtgct gtgccagccc cagcgccagt gcgccgccga
    12721 ccattcctcc gccgacaatg gcaatttccg ttggttgatt tgtcatggtc aatcatcctg
    12781 ttagcaatat gctttaagtt tacaggattt ttacccccaa gggctgataa cgctcatact
    12841 ggtcacaacg gcagcaaagc attacactat gcgccctgca ttcctggcta ctatttcgca
    12901 agagcaagtc gatgaccaaa aaactccata ttaaaacctg gggctgtcag atgaacgagt
    12961 acgattcatc gaagatggcc gatctgctgg atgcctccca cggctatcaa ctgaccgacg
    13021 tggcggaaga agcggatgtg ctactgctga acacctgctc aatccgcgag aaggctcagg
    13081 aaaaagtctt ccatcagttg ggtcgctgga aactgttaaa agagaagaat ccagacctga
    13141 ttatcggcgt cggtggctgc gtggcatcgc aagaaggcga gcacattcgc cagcgcgccc
    13201 actatgtcga tattattttt gggccgcaaa cgctgcaccg tctgccggag atgatcaact
    13261 ccgtgcgcgg cgaccgcagc ccggttgtag atatcagctt cccggaaatc gagaagtttg
    13321 accgtctgcc ggaaccgcgc gccgaagggc cgaccgcgtt tgtctccatc atggaaggct
    13381 gcaataaata ttgcacctac tgcgtggtgc cttacacccg tggtgaagag gtaagccgtc
    13441 cgtctgacga tattctgttt gagattgccc agcttgcggc tcagggcgtg cgtgaagtca
    13501 acctgctcgg tcagaatgtg aatgcctggc gtggtgagaa ctacgacggc accaccggat
    13561 cgtttgccga tctgttgcgt ctggttgctg cgatcgacgg gatcgatcgt attcgcttta
    13621 ccaccagcca tccgatcgaa ttcaccgacg atatcatcga agtgtaccgc gacacgcctg
    13681 agctggtgag cttcctgcat ctgccggtac agagcggttc cgatcgcatt ctgaacctga
    13741 tgggacgtac ccatacggcg ctggagtaca aagcgatcat ccgtaaactg cgtgcggcgc
    13801 gtccggatat tcagatcagt tctgacttca ttgttggctt ccctggcgaa accaccgaag
    13861 acttcgagaa aacgatgaag ctgattgccg acgtcaattt cgacatgagc tacagcttta
    13921 tcttctctgc acgtccagga acgcctgccg ccgatatggt tgatgatgtt ccggaagaag
    13981 agaagaagca gcgtctgtat attctgcaag aacgtatcaa tcagcaagcg atggcatgga
    14041 gccgtcggat gctcggcacc acccagcgta ttctggtaga agggacttcg cgtaagagca
    14101 tcatggagct ttccgggcgt accgaaaata accgcgtggt caacttcgag ggcacaccgg
    14161 atatgatcgg taaattcgtc gatgtagaaa ttaccgacgt ctacccgaac tctctgcgcg
    14221 gtaaagtggt gcgaactgaa gatgaaatgg gtctgcgcat ggcagaaaca ccggagtcgg
    14281 tgattgcccg cacccgcaaa gaaaacgacc ttggcgtggg ttattatcag ccgtaattct
    14341 caggcctgcc gttccggtgg gccttgtttt ttctctcttt atccccatct ttatcgcaat
    14401 gcttgcgcct ttttcctgtg gcgtgaataa tttccttata ggccggtgaa tgttcagctt
    14461 ccggcggcat acagataaga ggaacggttt gaacatagac actcgcgaaa tcaccctgga
    14521 gccagcagac aatgcgcgtc tgttgagcct gtgcggcccg tttgatgaca acatcaagca
    14581 gctcgaacgc cgtctcggca tcgagatcaa tcgccgcgat aaccacttta aactgaccgg
    14641 ccgtccgatt tgcgtcaccg ctgcggcaga cattctgcgt agcctgtatg tcgatactgc
    14701 cccgatgcgc ggtcagattc aggatatcga accggaacag atccaccttg cgattaaaga
    14761 agcgcgggta ctggagcaaa gcgcggagag cgtgccggag tacggcaaag cggtcaatat
    14821 caaaactaaa cgcggcgtaa ttaagccgcg cacgccaaac caggcgcagt acatcgccaa
    14881 tattctcgac catgacatta ccttcggcgt tggcccggcg ggtacgggta aaacctacct
    14941 ggcagtggct gcggcagttg atgccctgga gcgtcaggaa attcgccgta ttctgctgac
    15001 tcgtccggcg gtcgaagccg gtgagaaact gggcttcctg cctggcgatt taagccagaa
    15061 agtagacccg tatctgcgcc cactgtacga cgcgctgttt gaaatgctgg gctttgagaa
    15121 agtcgagaaa ctgattgagc gcaacgttat tgaagtcgcg ccgctggcct atatgcgtgg
    15181 tcgtacgctg aacgacgcgt ttatcattct cgatgagagc cagaacacta ccatcgaaca
    15241 gatgaagatg ttcctgaccc gtatcggttt taactcaaaa gcggttatca ccggcgacgt
    15301 cacacagatc gacctgccgc gtaatactaa atcaggctta cgtcatgcca ttgaagtgct
    15361 ggccgatgtc gaagagatca gctttaactt cttccacagc gaagacgtgg ttcgtcaccc
    15421 ggtggtggcg cgtatcgtta acgcttatga agcctgggaa gaagcagaac aaaaacgtaa
    15481 agcggcgctg gcagcagaac gcaagcgcga agaacaggaa caaaaatgag tcaggtgatc
    15541 ctcgatttac aactggcatg tgaagataat tccgggttac cggaagagag ccagtttcaa
    15601 acatggctga atgcggtgat cccgcagttt caggaagagt cggaagtgac tattcgcgtg
    15661 gtcgataccg ccgaaagcca cagtctgaat ctgacctatc gcggtaagga taagccgacc
    15721 aacgtgctct ccttcccgtt tgaagtgccg cctggcatgg agatgtcgct actgggcgat
    15781 ctggttatct gccgtcaggt ggttgagaag gaagctcagg agcaaggcaa accactggag
    15841 gcgcactggg cgcatatggt ggtgcacggc agtctgcatt tgttaggtta cgatcacatt
    15901 gaagatgacg aagcagaaga aatggaagcc ctcgaaacag agattatgct tgctctgggc
    15961 tatgaggatc cgtacattgc cgagaaagaa taatttgcca ggcatttgac tggcagacgt
    16021 ccccgccgtt ggtgattaac gacggcgttt tgattaacta acatgagaac ccatacgacg
    16081 ccatgagcga cgacaattca cacagtagtg acacgataag caacaagaag ggatttttct
    16141 ccctgttact cagccaactt ttccacggtg aaccgaaaaa ccgtgacgaa ctgctggcgc
    16201 tgatccgtga ttccgggcag aacgacctta tcgacgaaga tacgcgcgat atgctcgaag
    16261 gggtgatgga catcgcagac caacgcgttc gcgacatcat gatcccccgc tcccagatga
    16321 ttaccctgaa acgcaaccag acgctggacg aatgccttga tgtcatcatc gagtccgccc
    16381 actcacgttt cccggtgatt agcgaagaca aagatcacat tgaagggatt ctgatggcga
    16441 aagacttgct gccgtttatg cgcagcgatg ctgaagcctt cagcatggac aaagtgttac
    16501 gtcaggcggt tgtcgttcct gaaagtaagc gcgtagaccg gatgctgaaa gagtttcgct
    16561 ctcagcgtta ccacatggcg atcgttattg acgaattcgg tggggtttcc ggtctggtga
    16621 ccattgaaga catcctggaa ctgattgttg gtgagattga agacgagtat gacgaagaag
    16681 atgatatcga cttccgtcag ctgagtcgtc atacctggac cgtgcgcgca ctggcttcca
    16741 ttgaagactt caacgaagcg ttcggcaccc actttagcga tgaagaagtc gacactatcg
    16801 gtggtctggt gatgcaggcg tttgggcatc ttccggcgcg tggcgaaact atcgacatcg
    16861 acggttacca gttcaaagtg gcgatggccg atagtcggcg tattattcag gttcatgtca
    16921 aaatcccgga tgactcaccc cagccaaagc tggatgaata aaaccgaaac tggatagata
    16981 actacatgga ttttgcctca ttaattgaac gccagcgcat tcgcctgctg ctggcgttat
    17041 tattcggtgc ctgcggaacg ctggccttct ctccttacga cgtctggcct gcggcgatta
    17101 tttcgctgat ggggcttcag gcgttgacct ttaaccgtcg cccactccag tctgccgcta
    17161 ttggcttttg ctggggattt ggcctctttg gcagcggtat taactgggtc tatgtcagca
    17221 tcgcgacctt tggcgggatg cctggcccgg ttaacatctt cctggtggtg ctgctggcgg
    17281 cgtatttgtc gctgtatacc ggactgtttg ctggcgtgct gtcgcgtctg tggccgaaaa
    17341 ccacctggct gcgcgtggcg attgccaccc ctgctctctg gcaagtgacc gagtttctgc
    17401 gcggttgggt gctgactggc ttcccgtggt tacagttcgg ctatagccag attgatggcc
    17461 cgttaaaagg gctggcaccg ctaatgggcg tggaagccat taacttcctg ctgatgatgg
    17521 ttagtggcct gctggcactg gcgttggtca aacgcaactg gcgtccgctg gtggtggccg
    17581 tcgtgctgtt tgccctaccc ttcccgctgc gttacatcca atggtttacc ccgcaaccgg
    17641 agaaaaccat tcaggtttcg atggttcagg gcgatattcc gcaatcgctg aaatgggacg
    17701 aaggccagct tcttaatacg ctaaagattt actacaacgc aacggcaccg ctgatgggca
    17761 aatcatcgtt gattatctgg ccggagtcgg cgataaccga tctggaaatt aatcagcaac
    17821 cgttcctcaa agcactggac ggtgagttgc gtgataaagg tagctcgctg gtaaccggga
    17881 ttgtcgacgc gcgtctcaat aagcagaacc gctacgatac ctacaacacc atcatcacgc
    17941 tgggtaaagg tgcaccgtac agctacgaat cagccgatcg ctataacaaa aaccatctgg
    18001 tgccgtttgg cgagtttgtc ccgctggagt cgattctgcg tccgttagca ccgttctttg
    18061 atctgccgat gtcgtcgttc agccgtgggc catatatcca gccgccgctg tcggcaaatg
    18121 gtattgagct tactgcggct atttgctacg agattattct cgccgagcaa gtgcgcgata
    18181 acttccgccc ggataccgac tacctgctga ctatctccaa cgatgcgtgg tttggtaaat
    18241 ctattggtcc atggcaacac ttccagatgg cgcgaatgcg tgcgctggag ctggcgcgcc
    18301 cactgttgcg cagcaccaac aacggcatta cggcggtgat tggcccgcag ggtgagattc
    18361 aggcgatgat cccgcagttc gcccgcgagg tgttaaccac taacgtgacg ccgaccaccg
    18421 gactcacacc atacgcacgt accggcaact ggccgctgtg ggtgctgacg gcactgtttg
    18481 gttttgctgc tgtgttgatg agtctgcgtc agcgacgtaa ataatccctt cttatcgtgc
    18541 cggaatacat cccatatttc ggcacgctca ccggatgcgg catcagtgca ttatccggat
    18601 aacaatattc tcctatccaa ctttggcaca tctattgctt tgttatacaa ggcaaagctt
    18661 gaaccagcat cagtacaacc cggttgcacc acagcaatcc accggtagca ccaaaagagt
    18721 gcattttaag aattttgctt cagaatggtg cggtgcacct cgcaaaaaca aacaataaaa
    18781 catcaatatc tttacattta gccaaactaa atgttaccta acaggcacaa cactgcacaa
    18841 taaagttgca gacgataaca acacaaacac tcacaacggg tatccatgcg ttcttaacgc
    18901 agaagataaa ggtgttggat atgcaattac gtaaacctgc cacagcaatc ctcgccctgg
    18961 cgctttccgc aggactggca caggcagatg acgccgcccc ggcagcaggc agtacgctgg
    19021 acaaaatcgc caaaaacggt gtgattgtcg tcggtcaccg tgaatcttca gtacctttct
    19081 cttattacga caatcagcaa aaagtggtgg gttactcgca ggattactcc aacgccattg
    19141 ttgaagcagt gaaaaagaaa ctcaacaaac cggacttgca ggtaaaactg attccgatta
    19201 cctcacaaaa tcgtattcca ctgctgcaaa acggcacttt cgattttgaa tgtggttcta
    19261 ccaccaacaa cgtcgaacgc caaaaacagg cggctttctc tgacactatt ttcgtggtcg
    19321 gtacgcgcct gttgaccaaa aagggcggcg atatcaaaga ttttgccgac ctgaaaggca
    19381 aagccgtagt cgtcacttcc ggcactacct ctgaagtttt gctcaacaaa ctgaatgaag
    19441 agcaaaaaat gaatatgcgc atcatcagcg ccaaagatca cggtgactct ttccgcaccc
    19501 tggaaagcgg tcgtgccgtt gcctttatga tggatgacgc tctgctggcc ggtgaacgtg
    19561 cgaaagcgaa gaaaccagac aactgggata tcgtcggcaa gccgcagtct caggaagcct
    19621 acggttgcat gttgcgtaaa gacgatccgc agttcaaaaa gctgatggat gacactatcg
    19681 ctcaggtgca gacctccggt gaagcggaaa aatggtttga taaatggttc aaaaatccaa
    19741 ttccgccgaa aaacctgaac atgaatttcg aactgtcaga cgaaatgaaa gcactgttca
    19801 aagaaccgaa tgacaaggca ctgaactaat tacaagaacc aggggcggaa attccagccc
    19861 tctcgattgt tacgtagcac ggacagacta tacgcctgat ggtcgttccc catcgggcct
    19921 gaaaaccgca atacgctggg taacaatctt cgagggtagc agttaacgct gctaccctct
    19981 tttttctgga gtagatttat gtctatagac tggaactggg gtattttttt acaacaagcc
    20041 ccgttcggca ataccaccta tcttggttgg gtctggagtg gttttcaggt gacgatcgct
    20101 ttgtcgatct gcgcctggat tatcgctttc ctcgtcggtt catttttcgg cattttacgt
    20161 accgttccaa accgttttct ctccggtctg ggtacgttgt atgtcgaact gttccgcaac
    20221 gtgccgttga ttgtgcaatt ctttacctgg tatctggtga tcccggagct gctgccggag
    20281 aaaatcggca tgtggtttaa ggccgagctg gatcccaata ttcagttttt cctttcatcc
    20341 atgctctgcc tggggctgtt taccgccgcc cgtgtttgcg aacaggtacg cgccgctatt
    20401 cagtcgctac cgcgtgggca gaagaatgcc gcgctggcga tgggcctgac gctaccgcag
    20461 acttatcgat acgtactgct gcctaatgct tatcgcgtta tcgtcccgcc gatgacttcc
    20521 gagatgatga acctggtgaa aaactccgcc atcgcctcga ccatcggtct ggtggatatg
    20581 gcggcgcagg cgggtaaatt gctcgactac tcggctcacg cctgggagtc gtttaccgcc
    20641 atcacgcttg cgtatgtgtt gattaacgct tttatcatgc tggtgatgac gctggttgaa
    20701 cgtaaagttc gcctgcccgg caacatgggg ggcaaataat gtacgagttt gactggagtt
    20761 ccattgttcc ttccctgcca tatctgctcg acgggctggt gatcaccctg aaaatcaccg
    20821 tcacggcagt cgtgattggt attttgtggg gtaccatgct ggcggtgatg cgcttgtcca
    20881 gcttcgcgcc tgtttcgtgg tttgccaaag cctacgttaa cgtattccgc tcgattcctt
    20941 tagtcatggt tttgctgtgg ttttacttga ttgtgccggg atttctgcaa aatgtgctgg
    21001 gattatcgcc aaaaaatgat attcgtttaa tttcggcgat ggtggcgttt tcgatgtttg
    21061 aagcggccta ctattcagag attatccgtg ccggtattca aagtatttcc cgtggtcagt
    21121 cgagcgccgc gctggcgttg gggatgactc actggcagtc gatgaaactg attattctgc
    21181 cgcaggcttt ccgcgcgatg gtgccgctgc tgctcactca gggcatcgta ctgttccagg
    21241 atacctcgct ggtgtatgtg ttatctctgg ccgatttctt ccgtaccgcc tcaaccattg
    21301 gtgagcgtga tggtacgcag gtcgagatga tcctgtttgc cggatttgtt tatttcgtta
    21361 ttagccttag cgcgtcgttg ttggtcagct acttgaaaag aaggacagca taatgattac
    21421 cctgaaaaat gtttcaaaat ggtatggtca ctttcaggtg ctgaccgact gctcaaccga
    21481 agtgaaaaaa ggcgaagtgg tggtggtttg cggcccgtct ggttccggca aatcaacgct
    21541 gattaaaacc gtcaacggcc tcgaaccagt gcagcaaggt gaaatcaccg tcgatggtat
    21601 cgtggttaac gacaagaaaa ccgatctggc aaagctgcgc tcccgcgtcg ggatggtatt
    21661 ccagcatttc gagctgttcc ctcatctgtc gattatcgaa aacctgaccc tggcgcaggt
    21721 gaaagtgctt aaacgcgata aagcgcccgc tcgtgaaaaa gcattgaaat tgctggagcg
    21781 cgtgggactt tccgcacatg ccaataaatt tccggcgcag ctttccggcg gtcagcagca
    21841 gcgcgtggca atcgctcgcg cattgtgtat ggctcctatt gcgatgctgt tcgacgaacc
    21901 gacatcggcg ctggatccgg agatgatcaa cgaagtgctg gacgtgatgg tggaactggc
    21961 gaacgaagga atgaccatga tggtggtgac ccacgaaatg ggctttgccc gcaaagtggc
    22021 gaatcgggtg atctttatgg acgagggtaa aattgtcgaa gactcgccga aagacgcttt
    22081 cttcgatgat ccgaaatcgg accgcgcaaa agacttcctc gcgaaaatcc tgcattaatc
    22141 actctggcgc acatacccaa ttgcgcgcca tctcacgctt tatcgtttcg cccctcgtat
    22201 ctgttacatc ctggcgttac ccttgtcgca aagaagcaca caacaaggag caacaatggc
    22261 actgccaatt ctgttagatt gcgacccagg tcatgatgac gctatcgcaa tagttctcgc
    22321 actcgcctca ccagagcttg atgtcaaagc aattacgtct tccgccggaa accagacacc
    22381 agaaaaaacc ttacgcaatg ttctgcgtat gctgaccttg cttaatcgca ccgatattcc
    22441 ggtggcaggc ggcgcggtaa aaccgttaat gcgtgagttg attatcgcgg acaatgtgca
    22501 cggcgaaagc ggtctcgacg gcccggcatt accggaaccg acgttcgcac cgcaaaactg
    22561 tacggcggtg gagctgatgg cgaaaacgct gcgcgaaagt gcggaacctg tcacgattgt
    22621 ttctaccgga ccgcaaacta acgttgcctt gctgctcaat agccatccgg aactgcatag
    22681 caaaattgcc cgtatcgtga ttatgggcgg cgcaatgggg cttggtaact ggacgcctgc
    22741 ggctgaattt aatatttacg ttgacccgga agcggcagaa attgtcttcc agtcagggat
    22801 cccggtggtg atggccggtc tggatgttac tcataaagcg caaatccacg ttgaagacac
    22861 cgagcgtttc cgcgcgattg gcaaccctgt ttcaaccatt gttgccgaac tgctggattt
    22921 cttcctcgaa tatcataaag acgaaaaatg gggctttgtc ggcgcaccgc tgcatgaccc
    22981 atgcaccatc gcctggctgt tgaaacccga gttgtttacc actgttgagc gctgggttgg
    23041 cgtggaaaca caggggaaat atacccaggg tatgacggtt gttgattatt attatctgac
    23101 tggcaataaa ccgaatgcca ctgtaattgt cgatgttgat cgtcagggct ttgttgattt
    23161 actggctgat cgtctgaaat tttacgctta acactcgtca ttgattaaga tcaccacgcg
    23221 ttacctgggt ggtcttaatc tatttatctc tttttctttc aagatataaa tgcaatggat
    23281 aatgcagaac tcgccatagg tatcgatctc ggcactacca atagtttaat tgccgtctgg
    23341 aaagacggtg ccgcgcaatt aattccaaat aagttcggtg aatatttaac accatccata
    23401 attagcatgg atgaaaataa tcatatttta gtcggaaaac cggctgtatc acggcgtact
    23461 tcgcatccgg ataaaacggc agcgttattt aaacgtgcaa tgggcagtaa taccaactgg
    23521 cggttaggca gcgacacatt taacgcgcca gaactgtcct ctttggtatt acgctcatta
    23581 aaagaagatg ccgaagaatt tctgcaacgt ccgattaaag atgtggtgat ctccgttctg
    23641 gcttatttca gcgatgaaca acgcaagcat acccgtttag cagcggactt agccgggtta
    23701 aatgcggtac gcttaattaa tgaacccaca gcagctgcga tggcgtatgg cctgcatacc
    23761 caacaaaata cccgttcgct ggtgtttgat ctcggtggcg gcacgtttga cgttacggtg
    23821 cttgagtacg ccacgccggt gattgaagtt cacgcctccg ctggcgacaa cttcctcggt
    23881 ggcgaagatt ttacccatat gttggtcgat gacgttttaa agcgcgcgga tgtcacaagg
    23941 accacgctta acgagagtga actggcagcc ttgtacgcct gtgtggaagc ggcaaaatgt
    24001 agcaatcaat cgccaatgca cattcgctgg tagtaccagg atgaaacgcg ggaatgcgaa
    24061 ttttacgaga acgaactgga agatttgtgg ttgccgctgc tcaatcgctt gcgagtgccg
    24121 attgaacagg cgttgcgcga tgcgcgtctg aagccgagtc aaatcgacag tctggtgctg
    24181 gttggcggcg cgtcacaaat gccgctggtg cagcgaatcg ccgtgcgtct gtttggcaaa
    24241 ttaccgtatc aaagttacga tccgagcacc attgtcgcgc tgggcgcagc aatccaggcc
    24301 gcctgccgct tacgcagtga agatattgaa gaagtgatcc tcactgatat ttgcccttac
    24361 tcgttgggcg ttgaggttaa ccgccagggc gtttccggca ttttctcgcc gattattgaa
    24421 cgaaacacca ctgtgcccgt gtcgcgtgta gaaacttatt caaccatgca cccggaacag
    24481 gattcaatta cggttaacgt ctatcaggga gaaaaccaca aagttaaaaa caacattctg
    24541 gtggaatcct tcgatgtgcc gttgaagaaa accggggctt atcagtcgat tgatattcgc
    24601 tttagttatg atatcaacgg gttgcttgaa gttgacgtgc ttctggaaga cggcagcgtt
    24661 aagtccagag tgattaacca cagcccggta acattgagcg cgcagcagat tgaagagagt
    24721 cggacgcggt tatccgcatt gaaaatttat ccgcgcgata tgctcatcaa tcgcaccttt
    24781 aaagccaaac tggaagagtt gtgggcgcgg gcgctgggtg acgagcgaga agagattggc
    24841 cgggtgatca ccgattttga tgcggcgctg cagtcaaacg atatggcccg cgtcgatgag
    24901 gtcaggcggc gggcgagcgt ttatttagcc attgagacct cataatcacg gctgccggat
    24961 gcggcgtaaa tgctgcatcc ggcatcggcg caaaaaacac atcgtcaact aaacaacatc
    25021 gcgattttgc cgagagcata acctataacc agcaccgtta aactgatttt gataatgctg
    25081 gtaatgctct gtggtttttc accctcatca tttttttctt catggttttc aggtgttggc
    25141 tcatcgacat cttcatcctc cggctcgagt tttactttcg tcaacagcat ccgggtgata
    25201 atggcctcta atggatgagc atctccgcct tccagccttg tattaagacg ggccagcatc
    25261 tcttgtttcg gaaacgcttc aagtactacc tgttccgcta cccgatacaa atccagtaac
    25321 ggtgaatctt cttctgtctc gctaccgact ctcgatttcc accacgccca tgctttgcgt
    25381 ttgaagaata acgactcaat aaacgattga cctaacggcg ataattttcc gctatgtaaa
    25441 atttccgccc ataacagccg cgtccgggcg ctctcaccag gaacatattt cagcggttga
    25501 ccttgcgcat ctaccatgct ccgcgcttcg ataaccatca ctaataacgc gaaataatcc
    25561 gggcaatgac gcttgctcca ctgcaacaac aaattatcag cttgtgtaga tgggtaactt
    25621 tcccagtacg cgccgagatc agcaagcagg ctttccccct cgccgcagta caaacgttgt
    25681 gcacactggt aataccgggc atcttcgctt tctggttctt ccgcttgcca ctgtttggct
    25741 acaggaatga gttccaccat acctgtttgc accgagctgt accagcgcaa cagtttgcga
    25801 tgtaactttg catcatcagg aatgaaccac ggtccttcca tcgccaaaaa cgcagtgaca
    25861 tagtcaggat ggtaacggca gcaacgttcc agcgcgaagt aatattcaac agtctggttc
    25921 tgtaacgcga cgggatattc cagaaggcta aggaaatcga aacaatcacc agcctgaatg
    25981 gcctcaagaa aagcctccct ttcttcctca tccattcctt cgctgtcatt aaacgaatgc
    26041 cagttcaaac gctctgccaa caaagaagca caatttaatg acagatatcg cgcttctata
    26101 gcgattgcac acagcggcca gcgtaattga tcgacatcct ccatgttcca ggtatttaat
    26161 tgctgaataa atttctgcca ggcggagggc tgaaaacgat cactttctga atccagtaat
    26221 gtcctgaatg cacgtagtat ttcatgttca gcggtggcat ctttttcttc atcatctgct
    26281 tcctcgacag gatttaccgc cagtcgcagc gcctcttcat aggcctggcg taatgcttta
    26341 aacccctgcg gatcggtttc gggatgacac aacggtaagc gagcaagata agcctggcgg
    26401 ataatgtcta tttgcgtcgt gctttcaatt tcgaggattt gccaacatgt tttcataggt
    26461 cgctcacatc cttaagcgtt accgggcatt caggctggac agttaatggc tgccagggaa
    26521 gcgtggcata gaaacggtcg cttcccgctg ccagctcttc gaacatccga ctcccggcgc
    26581 tatcgtattt gtacagggtt agcgcctcgc gtagcgaagc gttgtcttcg cactgagaag
    26641 attgccagta gagacgccct agtgaatagg cagaaaaata gtgcatccag ctgtgatagt
    26701 aatggtgagc tcgtgcatag acgcgcgatt gcagccataa actctcttct tccgtcagcc
    26761 agttgttgag cacaccggcc cgtgacagaa atcccattcg aacataatcc caggctttga
    26821 ttcccccttc tccacagcac atagcggttt gggcaacgaa ttgggtataa atcaaccctc
    26881 tctccgagcc accagcaatt aatgctttcc actcttcagg ggaagagcgt tgccatgtca
    26941 gatataagcc agctaaatca ttggcatgac catcatcagt catctgataa atagtctgaa
    27001 ttaaatcatc acgattattt attccccagg atgatgagag atcgaattct tttatcccgg
    27061 tatacatttt tggcgagtca tgcgcgggtt tgcaactctg attaagtatt tccatgggcg
    27121 cagaaagcgc aaaaagaaga tattgttctt ccttattcat tttttaataa tcccttaagt
    27181 gcattaactg actacttttt tattattgat gagtataaac ttgacgcttt caaaataaaa
    27241 agaaaatcga agcattcaca catgaataaa aaattaatgt atatattcgc aatttttata
    27301 gttgcagcaa ttacctgtat tagccaaccc aagaaaacga tgttgcgtga taaagccatg
    27361 gtgaattatg cctttgatta tttaagatca ccaggcagtc taccgttaac cacggcagcc
    27421 acggagcttt ccgcgattca tggtcactca acatcgcaat atcgccttgg cgaattttat
    27481 cttcatggta gcgacggtaa accactggat tatacacagg cgagatactg gtatgagcaa
    27541 tcagcagaac aggaaaatcc gcgcgcacaa agcaaactgg ggtggatcta cctgaaaggc
    27601 ctgggagtca aacccgatac ccgtaaagca attctctggt ataaggaagc agctgaacaa
    27661 gggtatgctc atgctcaata tactttgggt ttgatctaca gaaatggcac tggtattaat
    27721 gttaaccatt atgaatctca aaaatggtta aaactggccg ccaaacaaca ttacaaaaat
    27781 gcggaaagat tacttgccgg acttcccgca cattaacgtc gactgtcacc cgaagagatg
    27841 gagtattttc cctatcaaac cggcaaagat aaaaatgtag aaaataattt tgatgacgcc
    27901 cagacagccc agcccgccat catcaacggg cattggctgc ggttgcggct ccggctcgac
    27961 ggtttcctga ccggtaaaat tcgccgcctg aattaataat cgacgaataa ttaatgcttc
    28021 cagtggacaa cgatcttctg catcaaggcg gctcacaatc ggcattaatg attcttctgt
    28081 cggttggctg gcctgcacta attgtttggc aactaaatat aatggccata agggatgttc
    28141 tgtatcacgc gtgagtgaga tccgcggctt acgtcctttg tgcaaaacca tctcgaccag
    28201 tgaacgcccc agcgcagaga gtttttcatc gctgagtatt tcgtataaat gaaaacgcgt
    28261 acgggcgctg tcgccgggga cataaagtag cggttttccc tgatcgttaa ccagatcacg
    28321 cgcttcaatc atcatcacca ctaatgggta ataatagacc cgatgctgac ggcaccattg
    28381 caacattaaa gcatccgcct gggtggaggg atactcgcgc cagtagtaac aaagttctgc
    28441 cagcaggctt tcgccttctc cacaatagac gcgttgtgcg tattcataat aacgcgcatc
    28501 ttcattatca ggttcagctt cccgccacga ccgggcgtaa tccaggagtt cggggattcc
    28561 caggcggctt gcgctatacc agcggagtaa atttctgtgc agttttgcat catcggggat
    28621 aatcactgcg cgatgttgcg ctaaatacaa tgtcagccat tctggatgcg atgaccaaat
    28681 ccgagcgaac atttggtaaa aatcgatggt gtcattttgc acggcaactg gcagatgcag
    28741 aatggtctgg aagttaaaaa cattcccctt cgcaatggca tataaaaagg attccagttc
    28801 ttcttcgtct atttccccga cgttttcctc ctgcaaccac cgcaatcttt ctgctaataa
    28861 caccacgcac tcgaaggata aatgggcagt gttcatggcg attgtgcaca gcgaccagcg
    28921 taattcatca atctcttcca tcgagcaata atttaattgc tgaatgaatc gctgccaggc
    28981 ggagggcaga aaacgttcgc tatcagaggc aagtaacgcg cgaaaggcga gcagaatttc
    29041 atgttctgct acctcatatt cttctggttg ccaaacagat ttagcaggcg actgcgcaat
    29101 ccgtagcgcc tcctcatacg cctgacgaag ttgtttaaaa ccctgcggat cggtttctgg
    29161 atgaaaggac ggtaacagcg ccagataagc gcggcggata atatcgacgt cagtcgtttc
    29221 ctctatatcg aggatcttcc agcaattttt cataagtcgc tcatatcctt aagcgtttcc
    29281 gggcatgtgg ggtagtcagc cagtggttgc caggacaagg tagggtaata cgcatcatca
    29341 ctggcaaata attgataaaa catatcattg ccggaatcgt cgtattcttt acgcgctaag
    29401 aattcgcgta ataaaggcag atgatcatct tcgggagact gccaatattg ccgaccaaag
    29461 gtataaccgg caaagtattg ccgccagtta ctataataac gtagagcccg tagatgaata
    29521 cgcgactgga tccatagact ttcttcttca ctcaaccagt tattaagtac gcccattcga
    29581 cttaaaaaac ccatccggac ataatcccat gctttaattc ctccctcacc acagcattcg
    29641 gctgtgctgg cgacaaattg cgcataagcc tgaccttgct catttaattc agcaagatag
    29701 tcacgccatt cacatggaga ataacgaaac cagcggtgat aaaaccctgc caaccgagcg
    29761 gcatgaccgt tctcgatcat tcggtgaatt atgcgcagca ggtcatcacg gtcatcaatt
    29821 ccccatgagg aagaaaggtc aaaaaagcat gtacctaaat acattttggg ggatgaatgc
    29881 gaaggtaaac agcattcatt gcgtatttcc atcggagtgg aaagtgcaaa caatattttt
    29941 tgcgacccca tgtccatata atttatcctt aaacattcat ttaatgaatg taaactacat
    30001 ttcgaggctc agttatttat acttttctga cgcaacataa tacgcataaa ataaggctta
    30061 cgcaaaaatg gaacacataa aaatataata tattttatct acaaccataa ggaaatgata
    30121 atgattttta cgtcaagttg ctgcgataat ttatcaatag atgagattat cgaacgtgct
    30181 gaaaaaggcg attgcgaggc tcaatatatt gttgggtttt attataatcg cgatagcgca
    30241 attgactctc cggacgacga aaaggccttt tactggctga agctggccgc tgagcaaggt
    30301 cattgtgaag cacagtattc cttagggcaa aagtataccg aggataaaag ccgtcataaa
    30361 gataatgagc aagccatctt ctggctgaaa aaagctgccc tacaaggcca tactttcgct
    30421 tccaacgccc ttggctggat actggatcgt ggagaagacc ccaactataa agaagcggtt
    30481 acctggtatc agatagccgc ggagagcgga atgtcttatg cgcaaaataa tcttggatgg
    30541 atgtacagaa atggcaacgg agtcgcacaa gactatacgc tggcattttt ctggtacaaa
    30601 caagctgcat tacaaggcca tagttacgca caagacaatc tggccgatct ttataaagac
    30661 ggagaaggcg ttgctcaaaa caagacactc gccgcattct ggtatttgaa aagcgcacag
    30721 cagggtaatc ggcacgccca gtttcaaatt gcatgggatt ataacgctgg cgaaggggtg
    30781 gaccaggact ataagcaagc gatgtactgg tatctgaagg ctgccgctca ggaaagcgtc
    30841 ggcgcttacg tcaacatcgg ttatatgtat aaacacggac aaggcgttga gaaagattat
    30901 caggctgcct ttgaatggtt tacgaaagcc gctgaatgca atgacgccac tgcctggtat
    30961 aacctggcca ttatgtatca ctacggagaa ggaagacctg tcgatctccg acaggctctc
    31021 gacctgtatc gtaaagttca gtcatccgga accagagatg tcagtcagga aattcgtgag
    31081 actgaagctt tactgtagaa ataaaaacaa gggccgggga atttcattct gcccatattc
    31141 tctttattgc tgtgcgaaat cagactatta cggctcaaac gggcgtctct ggaactggtc
    31201 ataaccacat ttcgggcata gcgtcagcac ttccggtgtg tagatcggga gatggaagtg
    31261 acatttctcg cagaccagat ttcccagccc gaccacttct ccgctgtgat aaaccccatg
    31321 atgattgagg tcctggaaaa tttcgcgcca ttcaagctgc gttttatcgg tgatgtctgc
    31381 cagctcctgc cacaagcttt ctttaatcac ccgcataaag acgctgtcag attcttcttt
    31441 caggctctct tcatagctca tggcgaactc ttccaggtca cgtctgacag ctcgcgtcag
    31501 ctcatcgatc tcggttcgcg ttaactcccc tgtttttatt acgcgctcgc gcgcctgttc
    31561 caccagtgcg tcgatatcac gttcgccatt gcgcaggcgt tcgttcagtg acgcgaccag
    31621 ttcacggtaa tattgagcaa ccttgttcat cgtttcatct cccgggtcgt taactgacta
    31681 taataataga cgttctttgt tccttgcttt gctaatacgc ccacaaactc tgtaaattaa
    31741 tgtacgaata atccgggcat catttcaacg tcgagtgcgc gaaaggctgt tttgacgcgg
    31801 acgtttgggc tatgctatgc ggatctgaaa aaccacctca acgctacatt tgtagccgta
    31861 ttgaaaacag gaccactggc tgccatgcaa gagcaatacc gcccggaaga gatagaatcc
    31921 aaagtacagc ttcattggga tgagaagcgc acatttgaag taaccgaaga cgagagcaaa
    31981 gagaagtatt actgcctgtc tatgcttccc tatccttctg gtcgactaca catgggccac
    32041 gtacgtaact acaccatcgg tgacgtgatc gcccgttacc agcgcatgct gggcaaaaac
    32101 gttctgcagc cgatcggctg ggacgcgttt ggtctgcctg cggaaggcgc ggcggtgaaa
    32161 aacaacaccg ctccggcacc gtggacgtac gacaacatcg cgtatatgaa aaaccagctc
    32221 aaaatgctgg gctttggtta tgactggagc cgcgagctgg caacctgtac gccggaatac
    32281 taccgttggg aacagaaatt cttcaccgag ctgtataaaa aaggcctggt atataaaaag
    32341 acttctgcgg tcaactggtg cccgaacgac cagaccgtac tggcgaacga acaagttatc
    32401 gacggctgct gctggcgctg cgataccaaa gttgaacgta aagagatccc gcagtggttt
    32461 atcaaaatca ctgcttacgc tgacgagctg ctcaacgatc tggataaact ggatcactgg
    32521 ccagacaccg ttaaaaccat gcagcgtaac tggatcggtc gttccgaagg cgtggagatc
    32581 accttcaacg ttaacgacta tgacaacacg ctgaccgttt acactacccg cccggacacc
    32641 tttatgggtt gtacctacct ggcggtagct gcgggtcatc cgctggcgca gaaagcggcg
    32701 gaaaataatc ctgaactggc ggcctttatt gacgaatgcc gtaacaccaa agttgccgaa
    32761 gctgaaatgg cgacgatgga gaaaaaaggc gtcgatactg gctttaaagc tgttcaccca
    32821 ttaacgggcg aagaaattcc cgtttgggca gcaaacttcg tattgatgga gtacggcacg
    32881 ggcgcagtta tggcggttcc gggtcacgac cagcgcgact acgagtttgc ctctaaatac
    32941 ggcctgaaca tcaaaccggt tatcctggcg gctgacggct ctgagccaga tctttctcag
    33001 caagccctga ctgaaaaagg cgtgctgttc aactctggcg agttcaacgg tcttgaccat
    33061 gaagcggcct tcaacgccat cgccgataaa ctgactgcga tgggcgttgg cgagcgtaaa
    33121 gtgaactacc gcctgcgcga ctggggtgtt tctcgccagc gttactgggg cgcgccgatt
    33181 ccgatggtga cgctggaaga cggtaccgta atgccaaccc cggacgacca gctgccggtg
    33241 atcctgccgg aagatgtggt gatggacggc attaccagcc cgattaaagc agatccggag
    33301 tgggcaaaaa ctaccgttaa cggtatgcca gcgctgcgtg aaaccgacac tttcgacacc
    33361 tttatggagt cctcctggta ctatgcgcgc tacacttgcc cagagtacaa agaaggtatg
    33421 ctggattccg aagcggctaa ctactggctg ccggtggata tctacattgg tggtatcgaa
    33481 cacgccatta tgcacctgct ctacttccgc ttcttccaca aactgatgcg tgatgcaggc
    33541 atggtgaact ctgacgaacc agcgaaacag ttgctgtgtc agggtatggt gctggcagat
    33601 gccttctact atgttggcga aaacggcgaa cgtaactggg tttccccggt tgatgctatc
    33661 gttgaacgtg acgagaaagg ccgtatcgtg aaagcgaaag atgcggcagg ccatgaactg
    33721 gtttataccg gcatgagcaa aatgtccaag tcgaagaaca acggtatcga cccgcaggtg
    33781 atggttgaac gttacggcgc ggacaccgtt cgtctgttta tgatgtttgc ttctccggct
    33841 gatatgactc tcgaatggca ggaatccggt gtggaagggg ctaaccgctt cctgaaacgt
    33901 gtctggaaac tggtgtacga gcacacagca aaaggtgatg ttgcggcact gaacgttgat
    33961 gcgctgactg aagatcagaa agcgctgcgt cgcgatgtgc ataaaactat cgctaaagtg
    34021 accgatgata tcggccgtcg tcagaccttc aacaccgcaa ttgcggcgat tatggagctg
    34081 atgaacaaac tggcgaaagc accgaccgat ggcgagcagg atcgcgctct gatgcaggaa
    34141 gcactgcagg ccgttgtccg tatgctcaac ccgttcaccc cgcacatctg cttcacgctg
    34201 tggcaggaac tcaaaggcga aggcgatatc gacaacgcgc cgtggccggt tgctgacgaa
    34261 aaagcgatgg tggaagactc cacgctggtc gtggtgcagg ttaacggtaa agtccgtgcc
    34321 aaaatcaccg tcccggtgga cgcaacggaa gaacaggttc gcgaacgtgc tggccaggaa
    34381 catctggtag caaaatatct tgatggcgtt actgtacgta aagtgattta cgtaccaggt
    34441 aaactcctca atctggtcgt tggctaagcg cgggaggaag cgtgcgatat ctggcaacac
    34501 tgttgttatc tctggcggtg ttaatcaccg ccgggtgtgg ctggcatctg cgtgacacca
    34561 cgcaggttcc ttccactatg aaggtcatga tcctggactc aggcgatccg aacgggccat
    34621 taagccgtgc ggtgcgtaac cagttacgtc tgaatggtgt cgagttgctt gataaagaaa
    34681 ccacgcgtaa ggacgttcca tccctgcgtt tgggtaaagt gagcatcgcg aaagataccg
    34741 catcggtatt ccgtaacggt caaacagcag agtatcagat gatcatgacg gtgaatgcga
    34801 ccgtgttgat ccccggccgt gatatctacc cgattagcgc caaagtcttc cgttcattct
    34861 tcgataaccc gcaaatggcg ttagcgaaag ataacgaaca agacatgatc gtaaaagaga
    34921 tgtacgaccg tgctgccgaa cagctgattc gtaagctgcc aagcatccgt gctgcggata
    34981 ttcgttccga cgaagaacag acgtcgacca caacggatac tccggcaacg cctgcacgcg
    35041 tctccaccat gctgggtaac tgatgattcg gttgtacccg gaacaactcc gcgcgcagct
    35101 caatgaaggg ctgcgcgcgg cgtatctttt acttggtaac gatcctctgt tattgcagga
    35161 aagccaggac gctgttcgcc aggtagctgc ggcacaagga ttcgaagaac accacacttt
    35221 ttccattgat cccaacactg actggaatgc gatcttttcg ttatgccagg ctatgagtct
    35281 gtttgccagt cgacaaacgc tattgctgtt gttaccagaa aacggaccga atgcggcgat
    35341 caatgaacaa cttctcacac tcaccggact tctgcatgac gacctgctat tgatcgtccg
    35401 cggtaataaa ttaagcaaag cgcaagaaaa tgccgcctgg tttactgctc ttgcgaatcg
    35461 cagcgtgcag gtgacctgtc agacaccgga gcaggctcag cttccccgct gggttgctgt
    35521 gcgcgcaaaa cagctcaact tagaactgga tgacgcggcg aatcaggtgc tctgctactg
    35581 ttatgaaggt aacctgctgg cgctggctca ggcactggag cgtttatcgc tgctctggcc
    35641 agacggcaaa ctgacattac cgcgcgttga acaggcggtg aatgatgccg cgcatttcac
    35701 cccttttcat tgggttgatg ctttgttgat gggaaaaagt aagcgcgcgt tgcatattct
    35761 tcagcaactg cgtctggaag gcagcgagcc ggttattttg ttgcgcacat tacaacgtga
    35821 actgttgtta ctggttaacc tgaaacgcca gtctgcccat acgccactgc gtgcgttgtt
    35881 tgataagcat cgggtatggc agaaccgccg gggcatgatg ggcgaggcgt taaatcgctt
    35941 aagtcagccg cagttacgtc aggccgtgca actcctgaca cgaacggaac tcaccctcaa
    36001 acaagattac ggtcagtcag tgtgggcaga gctggaaggg ttatctcttc tgttgtgcca
    36061 taaacccctg gcggacgtat ttatcgacgg ttgatatgaa atctttacag gctctgtttg
    36121 gcggcacctt tgatccggtg cactatggtc atctaaaacc cgtggaaacg ctggcgaatt
    36181 tgattggtct gacgcgggtc acaatcatcc ctaataatgt tcctccgcat cgtccccagc
    36241 cggaagcgaa cagcgtgcag cgtaaacaca tgcttgaact ggcgattgcc gacaagccat
    36301 tatttactct tgatgaacgc gagctaaagc gcaatgcccc ctcttacact gcgcaaacac
    36361 tgaaagagtg gcggcaggaa caaggaccgg acgtgccgct ggcgtttatt attggtcagg
    36421 attcattgct gacctttccg acctggtacg aatacgaaac gatactcgac aatgcacatt
    36481 tgatcgtctg tcggcgtcca ggttacccac ttgaaatggc gcaaccgcaa taccagcaat
    36541 ggctggaaga tcatttgaca cataacccgg aagatcttca ccttcagcct gccggtaaaa
    36601 tttatctggc tgaaacgccg tggtttaaca tctcggcgac catcatccgc gaacgtttgc
    36661 aaaacggtga atcgtgtgag gatttattgc cggaaccggt attgacttac attaaccaac
    36721 aaggcttgta tcgctgatac ctgtcgttac ggagggcatg atgcgactgt ggttaattcg
    36781 tcatggcgaa acacaagcga atgtcgatgg tctgtacagc ggtcatgcgc ccacacccct
    36841 gaccgcacgc ggtattgagc aagcgcaaaa tctgcataca ctgctagatg atgtttcctt
    36901 tgacttggtt ttatgcagtg aactggaacg ggcacagcat accgcgcgac tggttctcag
    36961 tgaccgccag ctccccgttc atatcatccc tgaactcaac gaaatgtttt ttggcgactg
    37021 ggagatgcga catcatcgcg acctcatgca agaagatgcc gaaaactata gcgcgtggtg
    37081 caatgactgg cagcatgcta tccccacgaa cggtgaagga tttcaggcat tttcgcaacg
    37141 tgtggaacgc tttatcgcaa ggcttagtga atatcagcac tatcagaata ttttaatcgt
    37201 cagccatcag ggcgtactga gtctgttaat tgctcgttta attggcatgc ctgccgagtc
    37261 tatgtggcat tttcgcgttg accaggggtg ttggagcgcc attgatatca accaaaaatt
    37321 cgcgacgcta cgcgtcctca atagccgtgc catcggggtc gagaacgcat gacttttctg
    37381 tttttttacg ggtaagccgc aacgaccatt gacagccccg ggcaggctga tattctccgc
    37441 agccagactt tttccgccag acacgacttt gtagaaattg ttttacaaaa atggcgatgc
    37501 aatctgcggc gcggggtggg atgatagccc actttcgaaa gccgattcgg cgacaattgt
    37561 cccgaaatcg cctctggttc aggtatactg acagaccatt tttatctatt tgattcaccc
    37621 agggggaaaa cttgcagggt aaagcactcc aggattttgt tatcgacaaa attgatgacc
    37681 tcaaaggtca ggacatcatc gccttagacg ttcaaggcaa atccagcatc accgactgca
    37741 tgatcatctg tacgggtacg tccagccgtc atgttatgtc cattgctgac cacgttgtgc
    37801 aggagtctcg cgcagcgggc ctgttaccgc tcggcgtaga aggtgaaaac agcgccgact
    37861 ggattgtcgt ggatttgggc gatgtgattg tccatgtcat gcaggaagag agccgtcgcc
    37921 tgtatgaact ggaaaaactc tggagttaat gcgtgaagct gcaacttgtc gccgtgggaa
    37981 cgaaaatgcc ggactgggta caaaccggtt ttaccgagta cctgcgtcgt tttccgaaag
    38041 atatgccctt cgagctgatt gaaattccgg ccggcaaacg cggcaagaat gcggacatca
    38101 agcgcatact cgacaaagag ggtgagcaga tgttggcggc cgcaggcaaa aaccgcattg
    38161 tcaccctcga tattccaggc aagccctggg atacgccgca gttagccgct gagctggaac
    38221 gctggaagct ggatggtcgc gacgtaagtc tactgattgg cgggcctgaa gggttgtcgc
    38281 ctgcctgtaa agcggcggct gagcagagct ggtcgctgtc ggcgcttacc ctcccccatc
    38341 cgctggttcg cgtgctggtc gcagagagtc tgtaccgggc gtggagcatc accaccaacc
    38401 atccttatca ccgtgagtga taagggagct ttgagtagaa aacgcagcgg atgaaactac
    38461 agaactcttt tcgcgactat acggcagagt ccgcgctgtt tgtgcgccgg gcgctggtcg
    38521 cctttttggg gattttgctg ctgaccggcg tgcttatcgc caacctgtat aatctgcaaa
    38581 ttgttcgctt taccgactac cagacccgct ctaatgaaaa ccgcattaag ctggtgccta
    38641 tcgcgcccag ccgcggcatt atctacgacc gtaacggtat ccctctggcc ctcaaccgca
    38701 ctatctacca gatagaaatg atgccggaga aagtcgataa cgtgcagcaa acgctggacg
    38761 ctttgcgcag cgtggtagat ctgaccgatg acgatattgc tgcattccga aaagagcgcg
    38821 cacgttcaca ccgtttcacc tctattccgg tgaaaactaa cctaaccgaa gtacaagtag
    38881 ctcgctttgc cgtcaatcag taccgttttc cgggtgtcga agttaaaggc tataaacgtc
    38941 gttactatcc ttacggttcg gcgttgaccc acgtcatcgg ctatgtgtcg aaaatcaacg
    39001 ataaagacgt cgaacgcctg aataatgacg gcaaactggc caactatgcg gcaacgcatg
    39061 atattggtaa gctgggcatt gagcgttact atgaagacgt gctgcacggt cagaccggtt
    39121 atgaagaggt tgaagttaac aaccgtgggc gtgttatccg ccagttaaaa gaagtaccac
    39181 cgcaagccgg acacgatatt tacctgacgc tggatctcaa actccagcaa tatattgaaa
    39241 cgctgctggc gggtagccgc gcagctgtgg ttgttaccga accgcgtaca ggtggggtgc
    39301 tggcgctggt ttccacgcct agttatgacc caaacctgtt tgttgacggt atctccagca
    39361 aagattattc cgccttgctg aacgacccga atacaccgct ggtgaaccgc gccacacagg
    39421 gggtttatcc tcccgcgtct acagttaaac cctatgtggc ggtttcggca ttaagcgccg
    39481 gggtgatcac gcgcaatacg acgctgtttg acccaggctg gtggcaacta ccagggtcgg
    39541 aaaaacgtta tcgtgactgg aaaaaatggg gccacgggcg tctgaatgtc acaagatcgc
    39601 tggaagaatc tgcggatacc ttcttctatc aggtggccta cgatatgggg atcgatcgcc
    39661 tctccgaatg gatgggtaaa ttcggttatg gtcattacac cggtatcgac ctggcggaag
    39721 aacgttccgg caacatgcct acccgcgaat ggaaacagaa acgctttaaa aaaccgtggt
    39781 atcagggtga caccattccg gttggtatcg gccagggtta ctggacagcg accccaatcc
    39841 agatgagtaa ggcactgatg atcctgatta atgacggtat cgtgaaggtt ccgcatttgc
    39901 tgatgagcac cgccgaagac ggcaaacagg tgccatgggt acaaccgcat gaaccgcccg
    39961 tcggcgatat tcattccggt tactgggagc tggcgaaaga cggtatgtac ggtgttgcta
    40021 accgccctaa tggtacggcg aataaatact ttgccagcgc accgtacaaa attgcggcga
    40081 aatctggtac cgctcaggtc ttcggtctga aagcgaacga aacctataat gcgcacaaaa
    40141 ttgccgagcg tttacgtgac cacaaactga tgaccgcctt tgcgccatac aacaatccgc
    40201 aagtggctgt cgccatgatt ctggagaacg gcggcgcggg tccggcggtt ggtacgctga
    40261 tgcgccagat cctcgaccac attatgctgg gtgataacaa caccgatctg cctgcggaaa
    40321 atccagcggt tgccgcagcg gaggaccatt aatcatgacg gataatccga ataaaaaaac
    40381 attctgggat aaagtccatc tcgatcccac aatgctgctg attttactgg cattgctggt
    40441 ttacagcgcc ctggttatct ggagcgccag cggtcaggat attggcatga tggagcgtaa
    40501 aatcggccaa atcgcgatgg gtctggtcat catggtggtg atggcgcaaa ttcctccacg
    40561 cgtttatgaa ggctgggccc cctatctcta tatcatctgt attattttgc tggtggcggt
    40621 agatgctttc ggagccatct ctaaaggcgc tcaacgctgg ctggacctcg gtattgttcg
    40681 ttttcagccg tcggaaattg ccaaaatagc cgtaccactg atggttgcgc gctttatcaa
    40741 ccgcgacgtt tgcccgccat cgttgaagaa tactggcatc gcgctggtgc tgatatttat
    40801 gcccacgctg ctggtggctg cacagcctga cctgggaaca tcaatcctcg ttgcgctttc
    40861 cggtctgttt gtactgttcc tctctggcct tagctggcgt ctgattggcg tcgcagtagt
    40921 gctggtagcg gcgttcattc cgattctgtg gttcttcctg atgcatgatt accagcgcca
    40981 gcgcgtaatg atgctcctgg acccggaatc agacccactc ggcgcgggct atcacattat
    41041 tcagtctaaa attgctattg gctccggcgg attacgcggc aaaggctggc tgcacggcac
    41101 tcagtcacag cttgaatttc tccccgaacg ccatactgac tttatcttcg cggtactggc
    41161 ggaagagctg ggattagtgg gcattctgat tctgctcgct ctctacattc tgctgatcat
    41221 gcgcgggctg tggatagccg ccagagcgca aaccaccttt ggtcgcgtca tggctggtgg
    41281 cttaatgctg atattattcg tttatgtctt cgtaaatatt ggtatggtaa gcggtattct
    41341 gccggttgta ggggttccgc tcccactggt cagttatgga ggatcggcgc taattgtgct
    41401 gatggctggg ttcgggattg taatgtcaat ccacacccac aggaaaatgt tgtcgaaaag
    41461 cgtgtaagag gtgcgcaatg cgtaagcagt ggctcgggat ctgcatcgcg gcaggaatgc
    41521 tcgcggcatg tacaagcgat gatggtcagc aacagacggt aagtgtaccg cagcctgcgg
    41581 tatgtaacgg ccccatagtt gaaattagcg gggcggaccc gcgtttcgaa ccactgaacg
    41641 cgacggcaaa tcaggattac cagcgcgacg gtaaaagcta caaaatcgtg caggatccgt
    41701 ctcgatttag ccaggcggga ctggcggcaa tctatgatgc cgaaccaggc agtaacctga
    41761 cggcctctgg cgaagctttc gatccgacaa agctgacggc ggcccatcca acgcttccga
    41821 tccccagcta cgccagaatc actaacctgg ctaacgggcg aatgatcgtg gtgcgcatta
    41881 atgatcgcgg cccttacggc aacgaccgcg ttatttcgct ttctcgcgcg gcagctgacc
    41941 gtcttaacac gtcaaacaac accaaagttc gtatcgatcc gattattgtc gcccaggatg
    42001 gttcgctttc tggtcctggt atggcttgta ccacagtcgc caaacagact tacgccctgc
    42061 ctgcacctcc cgatttaagc ggtggcgcgg gaacaagttc agtgtctggc ccgcagggtg
    42121 acattcttcc ggtcagtaat tcgacgctaa aaagcgaaga tccgaccggc gcgccggtaa
    42181 ccagtagcgg tttcctcggc gctccaacga ccttagcgcc tggtgtactg gaaggcagcg
    42241 aaccgacgcc tgcgccacag cccgttgtta cagcttcgtc gacaacgcct gcaacctcgc
    42301 ctgcaatggt gacaccgcaa gccgcctcgc aaagcgccag cggcaacttt atggtgcaag
    42361 tcggggccgt aagcgatcag gctcgtgcgc aacagtacca acagcaactg ggacagaagt
    42421 tcggcgtccc cggtcgcgta actcaaaatg gcgcggtctg gcggatccag cttggcccat
    42481 tcgccagcaa agccgaagcc agtaccttgc agcaacgttt gcaaaccgaa gcccaattac
    42541 agtcatttat tactaccgcg cagtagcgta aagcaggcat ctgatgtgtc aatttctgta
    42601 aatgacgtta acaaagtcac gcggaaagtc agatgcctgc cggtagtgca tttgctatag
    42661 tagggcactt ttttaattcc atcacggatg tcgtagttca gaccatgaat accatttttt
    42721 ccgctcgtat catgaagcgc ctggcgctca ccacggctct ttgcacagcc tttatctctg
    42781 ctgcacatgc cgatgacctg aatatcaaaa ctatgatccc gggtgtaccg cagatcgatg
    42841 cggagtccta catcctgatt gactataact ccggcaaagt gctcgccgaa cagaacgcag
    42901 atgtccgccg cgatcctgcc agcctgacca aaatgatgac cagttacgtt atcggccagg
    42961 caatgaaagc cggtaaattt aaagaaactg atttagtcac tatcggcaac gacgcatggg
    43021 ccaccggtaa cccggtgttt aaaggttctt cgctgatgtt cctcaaaccg ggcatgcagg
    43081 ttccggtttc tcagctgatc cgcggtatta acctgcaatc gggtaacgat gcttgtgtcg
    43141 ccatggccga ttttgccgct ggtagccagg acgcttttgt tggcttgatg aacagctacg
    43201 ttaacgcact gggcctgaaa aatacccact tccagacggt acatggtctg gatgctgatg
    43261 gtcagtacag ctccgcgcga gatatggcgc tgatcggcca ggcattgatc cgtgacgtac
    43321 cgaatgaata ctcgatctat aaagaaaaag aatttacgtt taacggtatt cgccagctga
    43381 accgtaacgg cctgttatgg gataacagcc tgaatgtcga cggcatcaaa accggacaca
    43441 ctgacaaagc aggttacaat cttgttgctt ctgcgactga aggccagatg cgcttgattt
    43501 ctgcggtgat gggtggacgt acttttaaag gccgtgaagc cgaaagtaaa aaactgctaa
    43561 cctggggctt ccgtttcttc gaaaccgtta acccactgaa agtaggtaaa gagttcgcct
    43621 ctgaaccggt ttggtttagt gattctgatc gcgcttcgtt aggggttgat aaagacgtgt
    43681 acctgaccat tccgcgtggt cgcatgaaag atctgaaagc cagctatgtg ctgaacagca
    43741 gtgaattgca tgcgccgctg caaaagaatc aggtcgtcgg aactatcaac ttccagcttg
    43801 atggcaaaac gatcgagcaa cgcccgctgg ttgtgttgca agaaatcccg gaaggtaact
    43861 tcttcggcaa aatcattgat tacattaaat taatgttcca tcactggttt ggttaaaaat
    43921 tgaacacttg aaagtgtaat ttccgtcccc atatactaag catcagtaaa aaaactcccg
    43981 ccttctggcg ggagttgcta tttaattacg ttacgccgga gctgacatga aaaccaaact
    44041 taacgaactg cttgaattcc ctactccttt tacttacaaa gttatggggc aggcgttacc
    44101 tgagctggtt gatcaggtgg ttgaagtggt acagcgccat gcgccaggtg actacacccc
    44161 aacggtaaaa ccaagcagca aaggcaacta ccactcggta tctatcacta tcaacgccac
    44221 tcatatcgag caggttgaaa cactgtatga agaactgggc aaaatcgata ttgtccgcat
    44281 ggttctgtaa ctcgcttctc cgttacccgg ctcctggtcg ggtaactccc ctctcctcgc
    44341 tgtgatatac ttttccccca cttttactca ttctccacgg agatgccgtt ttgtatcagg
    44401 ataaaattct tgtccgccag ctcggtcttc agccttacga gccaatctcc cgggctatgc
    44461 atgaattcac cgatacccgc gataatagta cccttgatga aatctggctg gtcgagcact
    44521 atccggtatt cacccaaggt caggcaggaa aagcggagca cattttaatg ccgggtgata
    44581 ttccggtgat ccagagcgat cgcggtgggc aggtgactta tcacgggccg ggacaacagg
    44641 tgatgtatgt gttgcttaac ctgaaacgcc gtaaactcgg tgtgcgtgaa ctggtgacct
    44701 tgcttgagca aacagtggtg aataccctgg ctgaactggg tatagaagcg catcctcggg
    44761 ctgacgcgcc tggtgtctat gtcggggaaa agaaaatttg ctcactgggt ttacgaattc
    44821 gacgcggttg ttcattccac ggtctggcat taaacgtcaa tatggatctt tcaccatttt
    44881 tacgtattaa tccttgtggg tatgccggaa tggaaatggc taaaatatca caatggaaac
    44941 ccgaagcgac gactaataat attgctccac gtttactgga aaatatttta gcgctactaa
    45001 acaatccgga cttcgaatat attaccgctt aattccatac atcaatggcc caatttacac
    45061 tgggtcatta ctcataatac ctgctaatgc tttacatctc cttcgcgttt gtatattttc
    45121 tttatcactt gcagaaatgc ggtgccacgc cttgctccgt aactcacccc attgattaac
    45181 ctattgaaat agttgattat ggtgagcaaa agcgttccgt tccaattaga cataagatta
    45241 ttgtaatttt ttaaatatta tttttccatg aataaaattg gagtaagtgc gtggatagta
    45301 ataatcaaat tgaaccctgt ttaagcagaa agtcgtcaga aggtaaacca caaatattta
    45361 caaccttacg aaatattgat cttaaccttc tgactatttt tgaagctgta tatgtacata
    45421 aagggatcgt taatgcagcg aaagtgctta atctgacccc ctcggcaatc agtcagtcta
    45481 ttcagaaact gcgcgttata ttccctgacc cattgtttat tcgcaaaggc cagggtgtca
    45541 ctcctaccgc atttgcgatg catctacatg agtatatcag tcagggcctt gagtccattc
    45601 ttggcgcgct ggatatcgaa ggaagctatg ataagcaacg aacgataact attgcgacca
    45661 ctccctcggt cggagccctg gtccttcctg tcatctatcg ggcgattaaa actcactatc
    45721 cgcagctttt actgcgcaac ccgcccatca gcgacgcgga aaaccaactc agtcagtttc
    45781 aaaccgatct catcatcgat aacatgtttt gcaccaatcg tacggtgcaa catcatgttc
    45841 tgttcaccga caatatggtg ttaatttgcc gcaagggaaa tccactactc tctttagaag
    45901 atgacagaga gactatcgac aacgctgcgc atgtacttct gttaccggaa gggcaaaatt
    45961 tcagcggtct gcggcagaga gttcaagaga tgtttccgga ccggcaaatc aatttcacca
    46021 gctacaacat tttgacaatc gctgcactgg ttgccaacag tgacatgtta gcgattattc
    46081 ccagccgttt ttataacctg tttagtcgtt gctggccgct ggaaaaactg ccttttccgt
    46141 ccttaaatga ggagcaaata gatttctcta tccactacaa caaattcatc ctgcgtgatc
    46201 ccatcctgca cggagtaata gatgttatcc gtaatgcatt ttgaaaaagt attgttacag
    46261 ccagatcaca gcgcagaacg ataaaatggc acaatccaac aacaatttta cattttcgcg
    46321 accgctttgg ctgctttcag gtccgtttca atgatatact gccagtcgtt aattcaaaaa
    46381 tagttgataa ttacaacaat ctattgaatt gaaacgcttt ccttcgtaat tcgcaactgg
    46441 aacacgtacg ctatgagtaa acccattgtg atggaacgcg gtgttaaata ccgcgatgcc
    46501 gataagatgg cccttatccc ggttaaaaac gtggcaacag agcgcgaagc cctgctgcgc
    46561 aagccggaat ggatgaaaat caagcttcca gcggactcta cacgtatcca gggcatcaaa
    46621 gccgcaatgc gcaaaaatgg cctgcattct gtctgcgagg aagcctcctg ccctaacctg
    46681 gcggaatgct tcaaccacgg cacagcaacg tttatgatcc tcggcgctat ttgtacccgc
    46741 cgttgtccgt tctgtgatgt tgcccacggt cgcccggtag ctcctgatgc caatgaacca
    46801 gtgaaactgg cgcagaccat tgccgatatg gcgctgcgtt atgtggttat cacctccgtt
    46861 gaccgtgatg acctgcgcga tggcggtgcc caacactttg cggattgtat tactgccatt
    46921 cgggaaaaaa gcccgcaaat caaaattgaa actctggtgc cggatttccg cggtcgaatg
    46981 gatcgtgctc tggatattct gactgcaacg cccccagatg tgttcaacca taacctggaa
    47041 aacgtaccgc gtatttaccg gcaggtacgg cctggcgcag attacaactg gtcgctgaag
    47101 ctgctggaac gctttaaaga agcgcatccg gaaatcccga ccaagtctgg tctgatggtg
    47161 ggtctgggtg aaaccaatga agaaattatt gaggtaatgc gcgacctgcg ccgtcatggt
    47221 gtgacgatgt taacgctggg gcaatatttg cagccaagcc gccatcacct gccggttcaa
    47281 cgttacgtta gcccggatga gttcgacgaa atgaaagccg aagcgctggc gatgggcttt
    47341 acccatgctg catgcggtcc gtttgtccgc tcttcttacc acgccgattt gcaggcgaaa
    47401 ggaatggaag ttaagtaatc ctgtaataat tacttacatc tgtctgataa aaaacccgct
    47461 ttttgaagcg ggttttttgt atcgaacaag atattgaggg agcgtcctgc tcgccacgtc
    47521 actctttatg agagagcttt tcagcctgaa gatcaacgtc tgcgcctttt ttcgccgcag
    47581 catcgtcatc attcatcgcc ttcttgaacc ctttaatggc cgctccaagg tctccgccca
    47641 gcgtacgtaa cttcttagtc ccaaacagca gaacgaccag cgccgcaact accagcagtt
    47701 tggtaatact aatctcaccc atagatacct tcttgacata taaccggcaa cagaacgcgc
    47761 cttaaaaacc agagaatatt aacggtcatt cgacgcgcgc acacaatagc aatctgtaac
    47821 aaggtgaatc aagcgccgag taaaaaaaca tcataataat tgcggcggcg caaagcgacg
    47881 gttgtttaag atgggcagtt gcgcgcgcac ctgacgcaca cgttcgggcg tcacttccgc
    47941 cataatgagt gcaggcattt ctgacgctgc cgcaatggtg acgccaaagg gatctataat
    48001 ccggctttgc ccgatatttt tgttcccgca ctcccccgcc gccaccatat aacaggtggt
    48061 atccagcgca cgagcggcaa gcaacgttga ccagtgatgc tctttgagcg gcccacgaac
    48121 ccaggcggca ggaagtacca ggatttcagc tccctgtaaa gcctgtgcca gcgccagctc
    48181 tggaaagcgt aagtcataac aggtcatcag accgaccttc atcccttcca cctccagtaa
    48241 cggagctatt tcattaccag catcaacatg gcgtgattcc tgaatggcaa atgcatcata
    48301 gagatgcagt ttggcataac gggcgacgat gtttcctgcc tgaagtgcca ccagcatatt
    48361 ccatgcgcgc cccggcgttg aaggaagatg aatcgtcaga attgtcgtca tcatgttacg
    48421 tttactttct cgccgtaaac gtccgaggaa ttcgccttcc agcagctgtg ctgatttaac
    48481 cgatagatct gcatcatgat catcgcgcgc cagcaatgct tccggcagga caaacagcga
    48541 tacgtcgttt tccgccgcct gcgccatcaa cgaggcacaa atctcagcgt tcttttccca
    48601 cacagatgta acagcaaact gtcctgcagc aaccagcatt gccacctcct gtcagagtcg
    48661 taaaattcgc caacgacgat tattcggcgt tacacttatc actcatacaa atcaaatagc
    48721 aggattttgc agtgttacaa cttcttttag cagtttttat tggcggtggt acgggaagcg
    48781 tagcgagatg gctgttaagt atgcgattta acccgctgca tcaggcgatt ccgttgggga
    48841 cgctggcagc aaacctgatt ggggcattca tcataggaat gggattcgcc tggttcagca
    48901 ggatgacgaa cattgatcca gtgtggaaag tattaatcac caccggattt tgtggcggtc
    48961 taacaacctt ctcaacattt tcggcagaag tggtgttttt gttacaagag ggccgctttg
    49021 gctgggcatt actgaacgtt ttcgtcaacc ttctggggtc ttttgccatg accgcactgg
    49081 cattctggct gttttcggcc tcaaccgtac actaaagacc aaaaaaaacc cgctgattaa
    49141 gcgggttttg aattcttgct gacgtatctt acagagcgat tacgtttgca gcagaagggc
    49201 ctttggcacc gttagtgatt tcgaactcta cgcgctgacc ttcagcaaga gttttaaaac
    49261 cattagtctg gattgcagag aagtgtacga acacgtcttt gctgccgtct tccggagtaa
    49321 tgaaaccgaa tcctttggac tcattaaacc acttaacgtt acctttaatc ttagacatca
    49381 aaattacctt tacatgaaaa atagacacaa atgctgtgtc ggttatcagt acaccaattg
    49441 tggtacgctt tgtccagtgc aactttacta aaaagtgata aaagtcgcgt tatttttgat
    49501 gttgcttatt ttagttattg attttaaaga agttactaaa acttcatttg tctcaaaact
    49561 gaaagcgcat ccaggcaaag tacacattgc cattgttgta ggtacccgga atgtaggtca
    49621 tctgaaaagt cactgggcca taacccacgg aggccaatgg cagtagaacc gggagaggga
    49681 tgtaattcca gttatcgcgt gccgttacgc cagcggtgaa tcccagacct aaatgaaaat
    49741 tttcatccgc cagcggtcgc caggtacttt cccatccgta tccggcaatc ggttcccatt
    49801 tgttccacga gtccttaaat gccatggcat acaggccatg ccagtttcct ttttcatccc
    49861 aacgcgacag gccaaaaccg ccaccccacg gtcgctcgtt atagcgatcg gttttttctt
    49921 tgtcgtaagc gaaacgtgca tgccaggtga tggcaggaat atataaatca taatgttcag
    49981 gctgttgcca ggtttgtgca atattttctc taaacgttgt catcctctca tctgcgttag
    50041 caaaaacttt accaacgctg attaactgaa taaaaacaaa ggaaaagata gcgacatatt
    50101 tactcacgtt catttgtgac cataaaacat ttatcaaaaa tctactacta gcatagcaaa
    50161 gctacaatta acataacctt aatagaccca acataaagaa taatctgaat agctgctcta
    50221 tttatttccg cttgttcaag ttaaatattt ccgcgcatcg attaaagatg atgcgtcgtt
    50281 ttctgcgatg ggaatagtca aaaaagaaaa accaagtctt ttttgatgac aaaatgcaat
    50341 caaggaaaaa ttattttatt tttttaacga gatacaacaa tcatcttaac gaagtatata
    50401 atatatatcg tcaacccatg ctcatattag attatttttt gagcgcacag ccgcagctaa
    50461 caccattgca attaacaaat ttgcatcaat ccaccatcaa tttacacaca ttattatgtg
    50521 ataattgcca accgctaaat atgcgttttg ttatctatgt ataaaaacag caacttcaat
    50581 gtcttaatgg cagtttttct tgattttaat cagcattcat cgccaattta ttgggcatat
    50641 tttttcctta agctttagga agtttttatt atttactttg ggcctggaga caggaaaaat
    50701 tatgctgaca ttcattgaac tccttattgg ggttgtggtt attgtgggtg tggctcgcta
    50761 catcattaaa gggtattccg ccactggtgt gttatttgtc ggtggcctgt tattgctgat
    50821 tatcagtgcc attatggggc acaaagtgtt accgtccagc caggcttcaa caggctacag
    50881 cgccacggat atcgttgaat acgttaaaat attactaatg agccgcggcg gcgacctcgg
    50941 catgatgatt atgatgctgt gtggctttgc cgcttacatg acccatatcg gcgcgaatga
    51001 tatggtggtc aagctggcgt caaaaccatt gcagtatatt aactcccctt acctgctgat
    51061 gattgccgcc tattttgttg cttgtctgat gtcactggcc gtctcttccg caaccggtct
    51121 gggggttttg ctgatggcaa ccctgtttcc ggtgatggta aacgttggta tcagtcgtgg
    51181 cgcagcagct gccatttgtg cctccccggc ggcgattatt ctcgcaccga cttcagggga
    51241 tgtggtgctg gcggcgcagg cttccgaaat gtcgctgatt gacttcgcct tcaaaacgac
    51301 gctgcctatc tcaattgctg caattatcgg catggcgatc gcccacttct tctggcaacg
    51361 ttatctggat aaaaaagagc acatctctca tgaaatgtta gatgtcagtg aaatcaccac
    51421 cactgccccc gcgttttatg ccattttgcc gttcacgccg atcatcggtg tactgatttt
    51481 tgacggtaaa tggggtccgc aattacacat catcactatt ctggtgattt gtatgctgat
    51541 tgcctccatt ctggagttcc tccgcagctt taatacccag aaagttttct ctggtctgga
    51601 agtggcttat cgcgggatgg cagatgcgtt tgctaacgtg gtgatgctgc tggttgccgc
    51661 tggggtattc gctcaggggc ttagcaccat cggctttatt caaagtctga tttctatcgc
    51721 tacctcgttt ggttcggcga gtatcatcct gatgctggta ttggtgattc tgaccatgct
    51781 ggcggcagtc acgaccggtt caggcaatgc gccgttttat gcgtttgttg agatgatccc
    51841 gaaactggcg cactcttccg gcattaaccc ggcgtatttg actatcccga tgctgcaggc
    51901 gtcaaacctg ggtcgtaccc tatcacccgt ttctggcgta gtcgttgcgg ttgccgggat
    51961 ggcgaaaatc tcaccatttg aagtcgtaaa acgcacctcg gtgccggtgc ttgttgggct
    52021 agtgattgtt atcgttgcta cagagctgat ggtgccagga acggcagcag cggtcacagg
    52081 caagtaaata gtaatgccgg aggcaagttt cctccggctt tatcatgtca cccactgtgg
    52141 tatatgcgtt gtggtctgcc aactttgccg tgaacaattt cagcaataat cagatggcgg
    52201 ctggcgcaat attcaagata acgcctggca gtggtgcggc tgatggtcag tgcctgcgcc
    52261 accgtttctg ccgtatgttg cacaccaggc tctttaaaca gttttcgcac cgcgtttagc
    52321 gtcaaggggt caatgccggt cggcagttcg tctttgggtt caccgcgagc ataggcatta
    52381 aacatctcat caatttgctt ctggctggcg ctatcaatac tttccagcat atgtttacgc
    52441 tggcggaaac gggttagcgt ttgccccagt gtattgattc acttgaagta cgaaaaaacc
    52501 gggagaacat tggattattc gggatctgat gggattagat ttggtggggc ttgcaagcct
    52561 gtagtacaaa ttttaggcgt taaccaatga aacgcgaaag atagtaaaaa attgcttttg
    52621 tttcattgaa aaaacgaaaa acaaaaacac tataaatcat ttcaatcata gcttcaaaaa
    52681 acgttcaaaa ccgataacaa ccaggctgtc accaaatgac tcatatcaca aatcagctca
    52741 tgccgtttag gtatgttaca tgtgtgatta tgtgaggtga agtatgtttt agctggctca
    52801 tggttgttat acggcttttt tcgcatcttg tggttccaac aaaggagcta caggctcttc
    52861 ctgtttatga acaccataca ttgacaaaat ctcttggcga gtttctggag gtaatttatg
    52921 cagcgccaaa gctgtttgta gtgtttggcg atcaataccc agcgttgcca atagggtgtc
    52981 aacggagatg gaggggcgtt cttgcattaa tccggaaagc ccatatttag ccatgtttag
    53041 ctctatctct cggacttcat tttctcctag tttattgatt aacagggaga acattttcaa
    53101 tgtatcagca ttcatttctt cgatagcaat tttttgcata gccggtattg ccgggatata
    53161 ttcaacccca ggcccgcgac gcccttcttg tgaacgcttc ggccatcctt ctttctttgc
    53221 tcgtttatgc accccctgta ttgtcccagg cattccatcc agttcagtaa tttgtttggg
    53281 tgtcatccac tggtttatct catttgcgct tttcataaac taagtttacc agatttatct
    53341 atataaaaat caatggatta caaaataaac cacattaact gatataaacc ggtttggttt
    53401 atttggtaat atataaaaca gataatcaag acgaattatt tgttttggta accctttatc
    53461 atacgcaata tggttggtgt gcgaaagatg agtaacgatt ggcatcgggc cgacgtgatt
    53521 gcggccctca aaaaaacagg aacccctctg gcaaaattgt ccagacgttc aggttattca
    53581 accgggacgc ttgcaaatgc attagtcagg ccatggccta aaggtgaacg cattattgct
    53641 gacgccttaa acatgaaacc tgaggaaatt tggcctagta gatatctaag ggatcacaat
    53701 gagagagtgg atatcagcaa ttgaatgcat gggtttatct ggtttacctg gttcattaat
    53761 gggggttcac aaaagagctc agagagaagg atggctcaag agaagaagag aaggaattag
    53821 aggggttgct tacgaatacc atatatcttc tcttcctaac attgctcgag ctgaagtttt
    53881 gctccaacaa ggaaaattta aaaccagtca gggatgtttt gaaatcgccc gccccacgct
    53941 ggaagctcat gattatgatc gtgaggcact atggagcaaa tgggataaag ccagcgattc
    54001 ccagcgcagc ctggctgaaa aatggttgcc ctcaattcag gccacagacg aaatgctgaa
    54061 ccaggggatt tcaacgaaaa cggcttttgc gaccgttgca gggcattacc aggtcagcgc
    54121 atccactttg cgggacaagt attaccaggt acagaagttt gcgaagcctg actgggcggc
    54181 tgcacttgtt gatggacgtg gagcatcccg tcgcaatgtt cacaaaagtg aatttgacga
    54241 ggatgcctgg cagtttctga ttgcagatta tctgcgaccg gaaaaacccg ccttccgcaa
    54301 atgttatgag cgtctggaac tggcagcccg cgagcatggc tggagtattc cctcccgtgc
    54361 cacggccttt cgccggattc agcaactgga cgaggcaatg gttgttgcct gtcgtgaagg
    54421 tgaacatgca ctgatgcatc tgataccggc acagcagcga actgtggaac acctggacgc
    54481 catgcagtgg atcaacggcg acggttatct gcataacgtc tttgtacgct ggtttaacgg
    54541 tgatgtgatc cgtccgaaaa catggttctg gcaggatgtg aaaacccgaa aaattctggg
    54601 ctggcgctgc gatgtgagcg agaacattga ttcaattcgc ctctcgttca tggatgttgt
    54661 gactcgctac agtatcccgg aggattttca catcaccatt gataacaccc gtggtgctgc
    54721 gaataaatgg ctgacgggag gcgcgcccaa tcgctaccgc tttaaggtaa aagaggacga
    54781 tccaaaagga ctgtttttac tgatgggtgc gaaaatgcac tggacaagcg ttgttgccgg
    54841 taaaggctgg ggccaggcaa aacctgttga acgtgctttc ggtgttggtg ggcttgagga
    54901 atacgttgat aagcatccgg cactggctgg cgcatatacg gggccaaatc cgcaggcaaa
    54961 acctgataac tatggcgacc acgctgttga tgcagagctg tttctgaaaa cctttgccga
    55021 aggtgtggcg atgttcaatg ccagaacagg ccgtgaaaca gaaatgtgcg ggggcaaact
    55081 ctcgtttgat gatgttttcg agcgtgaata cgccagaacg attgtgcgta agccaaccga
    55141 agaacaaaaa cggatgctgt tactgcctgc cgaggcggtg aacgtttcac gcaaaggcga
    55201 gtttacgctt aaagttggcg gctcccttaa aggcgcgaaa aacgtttatt acaacatggc
    55261 attaatgaat gccggagtga aaaaagttgt ggtcagattt gatccacagc agttacacag
    55321 cacggtttat tgag
//
LOCUS       DAEBXN010000015        53315 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.015, whole genome shotgun sequence.
ACCESSION   DAEBXN010000015 DAEBXN010000000
VERSION     DAEBXN010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 53315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53315
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.015"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(11..322)
                     /locus_tag="KJL99_000885"
     CDS             complement(11..322)
                     /locus_tag="KJL99_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HBD4435715.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            485..581
                     /gene="ffs"
                     /locus_tag="KJL99_000886"
     ncRNA           485..581
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="KJL99_000886"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            701..1054
                     /locus_tag="KJL99_000887"
     CDS             701..1054
                     /locus_tag="KJL99_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HBD4435716.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="KJL99_000888"
     CDS             complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="KJL99_000888"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HBD4435717.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPELGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FLEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDCLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(2810..3280)
                     /locus_tag="KJL99_000889"
     CDS             complement(2810..3280)
                     /locus_tag="KJL99_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HBD4435718.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(3396..3947)
                     /gene="maa"
                     /locus_tag="KJL99_000890"
                     /pseudo
     CDS             complement(3396..3947)
                     /gene="maa"
                     /locus_tag="KJL99_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
     gene            complement(4119..4337)
                     /gene="hha"
                     /locus_tag="KJL99_000891"
     CDS             complement(4119..4337)
                     /gene="hha"
                     /locus_tag="KJL99_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HBD4435719.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="KJL99_000892"
     CDS             complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="KJL99_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HBD4435720.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQALQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(5283..8432)
                     /locus_tag="KJL99_000893"
     CDS             complement(5283..8432)
                     /locus_tag="KJL99_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HBD4435721.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWLNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSTPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(8455..9648)
                     /gene="acrA"
                     /locus_tag="KJL99_000894"
     CDS             complement(8455..9648)
                     /gene="acrA"
                     /locus_tag="KJL99_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HBD4435722.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQGYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATVLATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            9790..10437
                     /gene="acrR"
                     /locus_tag="KJL99_000895"
     CDS             9790..10437
                     /gene="acrR"
                     /locus_tag="KJL99_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HBD4435723.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            10565..13927
                     /gene="mscK"
                     /locus_tag="KJL99_000896"
     CDS             10565..13927
                     /gene="mscK"
                     /locus_tag="KJL99_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HBD4435724.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(14139..14300)
                     /gene="rsmS"
                     /locus_tag="KJL99_000897"
     CDS             complement(14139..14300)
                     /gene="rsmS"
                     /locus_tag="KJL99_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HBD4435725.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTHDDEAEK"
     gene            complement(14314..14841)
                     /gene="priC"
                     /locus_tag="KJL99_000898"
     CDS             complement(14314..14841)
                     /gene="priC"
                     /locus_tag="KJL99_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HBD4435726.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQSCLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKISRWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            14911..15288
                     /locus_tag="KJL99_000899"
     CDS             14911..15288
                     /locus_tag="KJL99_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HBD4435727.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            15441..15992
                     /gene="apt"
                     /locus_tag="KJL99_000900"
     CDS             15441..15992
                     /gene="apt"
                     /locus_tag="KJL99_000900"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HBD4435728.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISENYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            16121..18046
                     /gene="dnaX"
                     /locus_tag="KJL99_000901"
     CDS             16121..18046
                     /gene="dnaX"
                     /locus_tag="KJL99_000901"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HBD4435729.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEGNGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTLVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     VKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    17379..17443
                     /gene="dnaX"
                     /locus_tag="KJL99_000901"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            18099..18428
                     /locus_tag="KJL99_000902"
     CDS             18099..18428
                     /locus_tag="KJL99_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HBD4435730.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            18428..19033
                     /gene="recR"
                     /locus_tag="KJL99_000903"
     CDS             18428..19033
                     /gene="recR"
                     /locus_tag="KJL99_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HBD4435731.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            19143..21017
                     /gene="htpG"
                     /locus_tag="KJL99_000904"
     CDS             19143..21017
                     /gene="htpG"
                     /locus_tag="KJL99_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HBD4435732.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWAKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            21198..21842
                     /gene="adk"
                     /locus_tag="KJL99_000905"
     CDS             21198..21842
                     /gene="adk"
                     /locus_tag="KJL99_000905"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HBD4435733.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKQVAEVRADLEKILG"
     gene            21974..22936
                     /gene="hemH"
                     /locus_tag="KJL99_000906"
     CDS             21974..22936
                     /gene="hemH"
                     /locus_tag="KJL99_000906"
                     /EC_number="4.99.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HBD4435734.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESSVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMVPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(22933..23892)
                     /gene="aes"
                     /locus_tag="KJL99_000907"
                     /pseudo
     CDS             complement(22933..23892)
                     /gene="aes"
                     /locus_tag="KJL99_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
     gene            24044..25348
                     /gene="gsk"
                     /locus_tag="KJL99_000908"
     CDS             24044..25348
                     /gene="gsk"
                     /locus_tag="KJL99_000908"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HBD4435735.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEVLYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVELVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSQEESYWDR"
     gene            complement(25481..27157)
                     /locus_tag="KJL99_000909"
     CDS             complement(25481..27157)
                     /locus_tag="KJL99_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HBD4435736.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(27395..28615)
                     /gene="fsr"
                     /locus_tag="KJL99_000910"
     CDS             complement(27395..28615)
                     /gene="fsr"
                     /locus_tag="KJL99_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HBD4435737.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYARLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            28833..30485
                     /gene="ushA"
                     /locus_tag="KJL99_000911"
     CDS             28833..30485
                     /gene="ushA"
                     /locus_tag="KJL99_000911"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HBD4435738.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNLEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGKTNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(30522..31001)
                     /gene="ybaK"
                     /locus_tag="KJL99_000912"
     CDS             complement(30522..31001)
                     /gene="ybaK"
                     /locus_tag="KJL99_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HBD4435739.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(31205..31999)
                     /locus_tag="KJL99_000913"
     CDS             complement(31205..31999)
                     /locus_tag="KJL99_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HBD4435740.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            32137..32478
                     /locus_tag="KJL99_000914"
     CDS             32137..32478
                     /locus_tag="KJL99_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HBD4435741.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNRVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(32693..35197)
                     /gene="copA"
                     /locus_tag="KJL99_000915"
     CDS             complement(32693..35197)
                     /gene="copA"
                     /locus_tag="KJL99_000915"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HBD4435742.1"
                     /translation="MSQTIDLNLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMIVTADNRSL
                     WLVIGLITLAVMVFASGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAIFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            35459..36391
                     /gene="glsA"
                     /locus_tag="KJL99_000916"
     CDS             35459..36391
                     /gene="glsA"
                     /locus_tag="KJL99_000916"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HBD4435743.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            36394..37686
                     /locus_tag="KJL99_000917"
     CDS             36394..37686
                     /locus_tag="KJL99_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBD4435744.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWGAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASGINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLDSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            37811..38218
                     /gene="cueR"
                     /locus_tag="KJL99_000918"
     CDS             37811..38218
                     /gene="cueR"
                     /locus_tag="KJL99_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HBD4435745.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(38219..38677)
                     /locus_tag="KJL99_000919"
     CDS             complement(38219..38677)
                     /locus_tag="KJL99_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HBD4435746.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(38674..39591)
                     /locus_tag="KJL99_000920"
     CDS             complement(38674..39591)
                     /locus_tag="KJL99_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HBD4435747.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            39737..40414
                     /gene="fetA"
                     /locus_tag="KJL99_000921"
     CDS             39737..40414
                     /gene="fetA"
                     /locus_tag="KJL99_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HBD4435748.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRDQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            40401..41180
                     /gene="fetB"
                     /locus_tag="KJL99_000922"
     CDS             40401..41180
                     /gene="fetB"
                     /locus_tag="KJL99_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HBD4435749.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGAIEFIPMQVIPVAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(41243..42097)
                     /gene="cnoX"
                     /locus_tag="KJL99_000923"
     CDS             complement(41243..42097)
                     /gene="cnoX"
                     /locus_tag="KJL99_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HBD4435750.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADKPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(42158..42967)
                     /gene="ybbO"
                     /locus_tag="KJL99_000924"
     CDS             complement(42158..42967)
                     /gene="ybbO"
                     /locus_tag="KJL99_000924"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HBD4435751.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPNDVERMNNMGFTGVLIDLDSPESVNRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLERLLPGRVMDKILQG"
     gene            complement(42957..43579)
                     /gene="tesA"
                     /locus_tag="KJL99_000925"
                     /pseudo
     CDS             complement(42957..43579)
                     /gene="tesA"
                     /locus_tag="KJL99_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
     gene            43550..44236
                     /locus_tag="KJL99_000926"
     CDS             43550..44236
                     /locus_tag="KJL99_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBD4435752.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            44233..46648
                     /locus_tag="KJL99_000927"
                     /pseudo
     CDS             44233..46648
                     /locus_tag="KJL99_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(47361..48455)
                     /gene="mnmH"
                     /locus_tag="KJL99_000928"
     CDS             complement(47361..48455)
                     /gene="mnmH"
                     /locus_tag="KJL99_000928"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HBD4435753.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMD
                     NDERASVGTCYKQQGSDAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAVWVKAQ"
     gene            complement(48524..49450)
                     /gene="allS"
                     /locus_tag="KJL99_000929"
     CDS             complement(48524..49450)
                     /gene="allS"
                     /locus_tag="KJL99_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HBD4435754.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSAGTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSCKAVEDIVTLFTQHRPEISGFLEIFGTPRS"
     gene            49680..50162
                     /gene="allA"
                     /locus_tag="KJL99_000930"
     CDS             49680..50162
                     /gene="allA"
                     /locus_tag="KJL99_000930"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HBD4435755.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            50240..51055
                     /gene="allR"
                     /locus_tag="KJL99_000931"
     CDS             50240..51055
                     /gene="allR"
                     /locus_tag="KJL99_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HBD4435756.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGRVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            51145..52926
                     /gene="gcl"
                     /locus_tag="KJL99_000932"
     CDS             51145..52926
                     /gene="gcl"
                     /locus_tag="KJL99_000932"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HBD4435757.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGVSHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGGCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            52939..53217
                     /locus_tag="KJL99_000933"
                     /pseudo
     CDS             52939..53217
                     /locus_tag="KJL99_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
     gene            complement(53231..>53315)
                     /locus_tag="KJL99_000934"
     CDS             complement(53231..>53315)
                     /locus_tag="KJL99_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBD4435758.1"
                     /translation="RKSLSFSKSVELHDKVIGHYLNIKHYQ"
BASE COUNT        12856 a        13715 c        14038 g        12706 t
ORIGIN      
        1 ccggagaggg ttagtagttc cagcgataac gctgcaagtc gatttgcccg cttcccgata
       61 ccatcacacc ttctgccagt aatgcctgtc gctgacgctg taaatccggc ccggttagcg
      121 aaattgtgcc atggcgatta accacccggt gccagggtaa ggtgctgcct tcagggagac
      181 gctttaacac accgcccacc tggcgcgcgg cgcggggcga tcccgccagt ttcgccacat
      241 caccgtaagt ggtgacatag ccttcgggaa tagcggcgac gatttgccag acgcgttggg
      301 gaaatgaatc ttctttttcc atcttttctt cctgaggtaa tttttcagca taatctggaa
      361 aaacgcccga gtgaagtcgc attgcgcaag aaaccagcat ctggcacgca atgggttgca
      421 attagccggg gcagcagtga taatgcgcct gcgcgttggt tttcaacgct ctcaatgggg
      481 gctctgttgg ttctcccgca atgctactct gtttaccagg tcaggtccgg aaggaagcag
      541 ccaaggcaga tgacgcgtgt gccgggatgt agctggcagg gcccccaccc atttctgcct
      601 cccaccgttt cgtcaaaaaa tcccagcatg gctaaacttt aaccacaact gacgtcgcaa
      661 gaattgtctg gctgcgcagt acgcttcgga ggtatgtctg atgaagtatg ttgatggttt
      721 tgtggttgcc gttcctgccg ataaaaagga tgcctatcgg gaaatggccg ctaaggccgc
      781 gccattgttt aaagagtttg gcgcgcttcg tattgtcgaa tgctgggcca gcgatgtgcc
      841 ggatggcaaa gtgaccgatt ttcgtatggc ggtgaaagcg gaagagaatg aagaggttgt
      901 ctttagctgg attgaatacc cttcaaaaga ggtccgcgac gcagctaatc aaaagatgat
      961 gtcggaccca cggatgaaag agttcggcga gtccatgccg tttgacggca agcgaatgat
     1021 ctatggcgga ttcgagtcaa tcatcgacga ataggtagcg tgacgggctg tgcggcgtgc
     1081 cgtcagccca aaaattcaca aatgttgctc ggcccagcgt aagaaatctt ctttcggtaa
     1141 tgccttgctg tagagccagc cctgaccgta gtgcacgcca tgctggcgta gccactcttc
     1201 ttgtttactg gtttcgattc cctccgctac cattttcagt ttcagtgttt ttgccatttc
     1261 gatgatatgc ggcgtgacat ttttatattc cagcgcatca acgaaagatt tatcgatctt
     1321 cagaatgtcg acatccagat cctgtaaata acttaaactt gaataccccg taccaaaatc
     1381 atcaagataa atttcatggc ccgcctcccg gtagcgagaa attatcgggg cgctggtttt
     1441 cggatcggca aactcgcgtt cagtgagttc aagcgcgatc tgtctgggat taacctgata
     1501 gtgattgatc atttcacgca gcaattgcgg gattttttcc gaggtgagca cggtggattc
     1561 aagattgatc gaaatatgct gctgtggatg ctgacgcaag cagtcgccca tatcttcaaa
     1621 gacgcttctt ataatcagta gcgtcaatgg ctcagaaagg cccgtttgct gtgccagcgg
     1681 aataaaacta tctggtgaca accaactacc gtctgtctgc ggccagcgcg ccagtgcctc
     1741 agcaccgaca attttgccat tggctaagga gacaatcggc tgatagtgca cgcaaatatc
     1801 acgattttcg atagcatcct gcagccgatg atgcggtgat tggatacggc gcaaaatacg
     1861 cagcacaaac atcgctgcca gcaggccaat caccaacccg agcggtaacc aaataaagac
     1921 ttgccgatgc cagcctttct gcaacatttt cgttgaagcc caggtgatga tcgaaatatt
     1981 catctccagg aagggcagta tatcgtaaat gatcccatta ttttcgatat gctcaccggg
     2041 tgttttttgt agcctggtaa taattccctg agcaatttca tcgctgctgg ttatgacaac
     2101 gttatgggca ttgccaataa tggcggcatc aatttgccat gaactatagg gaatgacatc
     2161 aataaaggaa gcggggtcga tcatgacgac ataatgtgcc gttcccatgg cgaccatgta
     2221 acggataatg cctaaatcgt tatgcgatgt taaccagaca cgataaccat ctttcgaaat
     2281 tttacctagc tcgggaaagg tatcgggcgg actttcatgc tccagagacg aacactgggg
     2341 aacgttgtta tcgatataaa ccacttcctg aatatagcga taactgtaag atacccgacg
     2401 catttccatg agatgggctt cgctacaggc agcgccttgc catctttcca gctcctgcag
     2461 cgcatctttc ccttgtgtcg ccaccttatt ggctcgaata gcgacgcggg aggaataggt
     2521 atccagctct tcaataaacg atgtttctac ctgctgatgg gccagccaga tgcttaagcc
     2581 gacaggcagc aatactgaaa gaatcagtac tcccgaaata aggccgacca gatgtcgtgt
     2641 tctcaccagc atatccttat taacacagtg tagggaaagt atatctgatt gtggaaaaag
     2701 gctggcagga gataaggagg tagagctggt atttcatgaa gtgatgggtg gaaacgatca
     2761 ttgtcatttg accaggcgca gagctttacg cctggtatgt aaccctaact taagatgttg
     2821 attcggcgcg agcgagggta aaaatatcgt aaaaagacag ttcaccttta cggacgatca
     2881 ttttttgcag ttgttcctta tgaacatccg cgacacgcgg agagttcaag atggcttcaa
     2941 ccagcgcggc aggtacaaag cgcgtgttgt accattcgcc gttatagcaa acgcggaaat
     3001 ccagcacgtc gatatcttca tagcggtagc gtgggtagac atcgaaaaag aagaaaccat
     3061 taagcaggat aaataataca accagtagcc agacagagcc tgacagcgtt tcggactgca
     3121 acatcaccgc cagggtggca aaaaaagcaa cgtacatacc gataaacagc cccggatgtt
     3181 tacggataaa actgatacta aagcggggtt tgttgtcgcg tttttcgcgg gtattcagag
     3241 actcaatcgt ttcggtcagc aggcgttgta tttcggtcat cgtttgcctt cgtggtatct
     3301 gcaactttcc agggaacacc ctattttaac gtgggaatag tagaggggaa gtaacagatc
     3361 taccacaatt ttgcataaca gttgcgaaaa accgattaca attttttaat tattctggct
     3421 ggattaccgc ccacgacaac gttgtccggg acaccttttg tgacaactgc acctgaagct
     3481 accacgacgt tatcaccaat ggtcacacca gggttaatga ccgcgcgtcc gccaatccag
     3541 acgttattac cgatggtgac gggtttcccc agttcagcac cgctattacg tgctacaggg
     3601 tcgatgggat gtgttgccgt gtagatatga acgcctggtg ccaacataca gttatcaccg
     3661 atgcgaatag ggcagacatc aagcatcacg caatcgaagt tggcgaaaaa attattgccg
     3721 agaaaaatgt tatagccata atcacagcga aacgttggct caatataagc ctctgtcacc
     3781 tgaccgaata aattagcgag aatttactgg cgtaatgtgt gctcttccgc cagggaatga
     3841 ttgtatcggt gaataagctg acgagcgcgc aggcgatcgc gagataacgt ctcatctgcc
     3901 gagcgataca actcaccagc aatcatcttt tctttttctg tgctcattac tgacctccgg
     3961 ttgctgaaaa tgcggcaaca cagtaagacg aaaagtcagt gaagagaacg ttctcattac
     4021 atctgtgtga taaagatcac atagggtatg tcttcatggc gaaaaagtat aaaattctta
     4081 ataaacagcc ggttatagca ccgaaagcga attatttatt agcgaataaa tttccatact
     4141 gaggaaggga tcttgtcgta cagtttattc atggtcaatt cggcgaggcg gtgatctgcg
     4201 gctgagtaaa ataccgccag ttcattatct gataattcgt atttattttt ctcgataaca
     4261 cgctccagcg tgtcaattgt ctggcaacga cgtaaacgca ttaaataatc ggtttttgtt
     4321 aaaggttttt cggacatact tctacccatg gttgtaataa ttctaacaag ataaactcgc
     4381 aggattctct ttcgtcgcgt tgacaaaaca acggaatagt cgattacctg acttccgcca
     4441 tttctgaaga gcctgcatat taataccata actgctgaac aacataaagg tgtcatccag
     4501 atattcgtcg atctgctcaa tgagcttatt gtcttcatta tacttaattt tgtaattaag
     4561 tgcgaaggtc gcaatatgct caatcagttc attcaactgg aggttgatcg ccgaggttgg
     4621 gtcgttaacc caaccatgat tgctttcttc aaggtttgca agacagtcat gatacagggt
     4681 ttcacagaga aacttaagct gtgcgatatc atgtcttttg ggtgagtatt catccataac
     4741 gcgtcccctt cttagtggtt gaactaacgg acacctttcg ggatggaaaa aacttactga
     4801 cctagacttg ccctctcgtt tgttagctta actataagcc actctttgca ggttttcatc
     4861 gcattattac gaaacattac aattaggaag gaatcgggac aggactcact ttttcatact
     4921 agcactaacc cgcagcaggt aaaagcagtt tgctcgaact gttcataaca tttcaggatt
     4981 atcgccagat gaaattggtg acactaatac caggattgct ctgaatatga cgtaataacc
     5041 gaggaatgaa taaagaatta ccgcaaataa ttaagaatag cctctaaatg attatggatt
     5101 attaaatcta ttagcgctac ttaatataaa ttaactaaca atcagtattt tttatgaatt
     5161 ttatacgtgg ttaatactgg ttttcgtatg agatcctgag ttggtggttc aattactcct
     5221 taatgttcgt aggttatgca taaaaaaggc cgcttacgcg gccttagtga ttacatgttg
     5281 tatcaatgat gatcgacagt atggctgtgc tcgatatctt cattcttgcg gctaaagcgg
     5341 cggcgaacca ccacaaagaa taccggaacg aagaagattg ccagtaccgt tgcggtcacc
     5401 atcccgccca ttacaccggt acctactgcg ttctgcgcgc cggaaccagc accagtactg
     5461 ataaccagcg gcataacgcc gaggataaac gccagcgagg tcatcaggat cggacgtaaa
     5521 cgcatccgca ccgcatcaag cgtcgcttca atcagacctt taccttcttt atccatcaag
     5581 tctttggcga attcgacgat aagtatcgcg ttcttcgccg acaacccaat ggttgtgagc
     5641 agtcctactt ggaagtaaac gtcattggtc aggccacgga aggtggcagc cagcaacgca
     5701 ccgataaccc ccagcggaac gaccagcata acggagaacg gagtcgacca gctctcgtac
     5761 agcgccgcca gacacaggaa tacgacaatc aacgaaatcg cgtacagtga aggtgcctgg
     5821 ttgccggaga gacgttcctg ataggacatc cccgtccagt catagccaac accggtaggc
     5881 agtttgctcg ccagttgttc catcagctcc attgcttcac cggtactttt acccggtgcc
     5941 gcctggccta agatttccat ggatggcagg ccgttgtaac gttccagacg cggcgaaccg
     6001 tactcccaac gagaagagga gaacgccgag aatggcacca tctgaccatc agcagcacga
     6061 acataccagt cgccgatatc atccggcagc atacggtatt tcgcttctga catgacgtaa
     6121 actttcttca cacgaccgcg gtcgataaag tcgttcacat agctaccgcc ccatgcagcg
     6181 cccagagtgg tgttaatgtc gttgatagaa acacccagcg cctgcgcttt ttcctggtcg
     6241 atatcaatct taaactgcgg ggtatcttcc agaccgtttg gacgtacgct ggtcagcata
     6301 tcagggtgct tcgctgcttc tgcaagcagc tggttacgcg cctgagtcag tttttcgtga
     6361 ccaaggccag cctggtcaat cagctcaaag tcaaagccgg ttgcagtacc cagttccacg
     6421 attgcgggca ggttaaaggc gaaaaccatc gcatctttga tttgcgagaa agcgcgtgtt
     6481 gcacgcatgg taatcgcttc aactttgttt tcttcgcccg gacgatcggc ccagtccttc
     6541 aaggaaacga acgcaatacc ggtattctga ccacgtcccg caaagccgaa gccgttaacg
     6601 gcgaacaccg actcaacgtt gttcttttct ttggtcagat agtaatgcgt tacctcattg
     6661 agcactttct gcgtacgttc ctgcgttgca cctgctggca gctgaaccat ggtcataaat
     6721 acgccctggt cctcatctgg caagaaggag cttggcagac gcacgaacag ataggccatg
     6781 ccgaccacga tgatcagata cagcactagg taacgccccg tactgcgcag aataccgcct
     6841 acgctgtcgg tgtagtggtg cgtgctcttc tcgaacatgc ggttcaacca gccgaagaag
     6901 ccttttttac cttccccgtg atcgcctttg gcaatcggtt tcagcatggt ggcacaaaga
     6961 gccggagtca ggatcaacgc taccagtacc gacagcgcca ttgctgaaac aatggtaata
     7021 gagaactgac gatagatagc accagtagaa ccgccaaaga aggccatcgg tacgaatacc
     7081 gccgacagta ccatcgcgat accgaccaga gcgccctgaa tctgccccat cgacttacgg
     7141 gtagcttctt ttggcggcaa accttcttcc gccataacac gctccacgtt ttctaccaca
     7201 acgatagcgt catccaccaa caagccgatg gcgagcacca tcccgaacat tgttagcgtg
     7261 tttatcgaga agccaaaggc ggcaaggacg gcaaaggtcc cgagcaatac caccggtacg
     7321 gcaatggtcg gaatcaacgt cgcgcggaag ttctgcagga acagatacat aaccaggaac
     7381 acgaggatga tcgcttcgac cagcgtttta accacttcgt gaatagagat tttcacgaac
     7441 ggcgtggtgt cgtacgggta aacaattttc agacccgacg ggaagaacgg ttccatcttc
     7501 gccagttcag cacggattgc cgcagcggta tccagcgcgt ttgcaccggt cgccagcttg
     7561 atccccagac cggaagcagg ttggccgtta aactctgcga tgatgtcgta gttctcacca
     7621 cccagctcaa tcttcgccac atcacgcagc agtacgcggg aaccatcctg attcactttc
     7681 agcaggattt tgccgaactc ttcagtagag gtcagacgcg tctgagcaat aatagaggcg
     7741 ttaagctgtt ggcctttcac cggcggcgta ccaccgagct gacccgccgc aacctgggcg
     7801 ttctgcgctt tgatggcggt aatgacatca accggcgtta gctggaattt gttcagctca
     7861 ttcgggttca tccagatacg catcgcgtac tgtgaaccga acaactgaac atcacccacg
     7921 cccgacgtac ggctgatggc atctttcata ttcgccgcta cgtagtcaga gatatcctcc
     7981 tgcgtcatgg tgccatcggt gttgataacg ccgacaacca tcaggaagct gctggatgat
     8041 ttctcaacgc tcaccccttg ctgctgaact tcttgcggca gcaacggcat cgccagctgc
     8101 agtttgttct gcacctgaac ctgcgcgata tccgcatcag taccagactc aaaggtcagg
     8161 gtgatctgca cggtacccgt ggagtcactg ttagaggaca tgtacatcag gttatcgata
     8221 ccgttcatat tctgttcgat aacctgtgtc accgtgtcct gcactgtttt cgcatcagcg
     8281 ccggggtagg aggcggagat cgttactgcc ggcggtgcaa tcgtaggata ttgcgccacc
     8341 ggcagtttga ggatcgccag cccccctgcc aacatgatga taatggcgat cacccacgca
     8401 aaaatcgggc gatcgataaa gaaattaggc atgtcttaac ggctcctgtt taagttaaga
     8461 cttggactgt tcaggctgag caccgcttgc ggcttgctgg ttattatcag cggtaacttc
     8521 ttgtgctttt acctggacac caggacgcac tttctgcagc ccacttatta ctacgcgatc
     8581 gcctgctttc agaccttctg tcaccagcca cttatcgcca atagcctggc ttgcaacgat
     8641 cggacgggtt tccactttgt catccgcgcc aactaccagt acggtggcat caccacgcgg
     8701 cgtacgggtt acgccctgtt gcgggactaa aatagcgttt ggattaagcc cttcttccag
     8761 acgtgcgcgc acgaacatac ccggcagcag agtgtgatcc gggttcggga agatagcgcg
     8821 tagggtgata gacccagtgg tctgatcaac ggtaacgtca gagaattcca gcgtaccgtc
     8881 ctgcgggaac ttaatgccgt cactggtgat cagcgacact ttggctttgc cgttctcttg
     8941 tttcagcgtg ccattcgcca gttcctgttt caggcgaagg aagtcgttgc tggactgggt
     9001 cacatcaacg tagatcggat caagttgctg cacggttgcc agcacagtcg cctgaccgtt
     9061 ctgtaccaat gcgccttccg tcacgttcga cttaccaatg cgaccgctaa tcggagaggt
     9121 gactttggtg taagccagat tgatccgcgc agtttcaacg gcagctttcg ccgcagttac
     9181 cgcagcattc gcctgttgcg catcagccag agcctgatcg tacccttgct tactgatgta
     9241 ctgagtaccg agcagtttct gataacgatt caccgtcaat tgcgcgatat tggctgcagc
     9301 ctgggctttc gccagatcac ctttcgcact gtcgtatgtc gcctgatagg tcgcaggatc
     9361 aatctgatag agagagacac cagcttcgat gtcgctacct tctttgaaat tacgcttcag
     9421 gataatcccg ctaacttgag gacgaacttc tgcgatccgg taggcactgg tgcgacctgg
     9481 aagctcggtt gtgatctgca gaggttcagt tttgactgtt actacgccaa cggcgggcat
     9541 ctgctggcca ccttgttggg cctgtttgtc gtcacatcct gttagggcta agctgcctga
     9601 gagcatcaga acgaccgcca gaggcgtaaa ccctctgttt ttgttcatat gtaaacctcg
     9661 agtgtccgat ttcaaattgg tcaatggtca aaagttaata aacccattgc tgcgtttata
     9721 ttatcgtcgt gctatggtac atacattcac aaatgtatgt aaatctaacg cctgtaaatt
     9781 cacgaacata tggcacgaaa aaccaaacaa gaagcgcaag aaacgcgcca acacatcctc
     9841 gatgtggctc tacgtctttt ctcacagcag ggggtatcat ccacctcgct gggcgagatt
     9901 gcaaaagcag ctggcgttac gcgcggtgca atctactggc attttaaaga caagtcggat
     9961 ttgttcagtg agatctggga actgtcagaa tccaatattg gtgaactaga gcttgagtat
    10021 caggcaaaat tccctggcga tccactctca gtattaagag agatattaat tcatgttctt
    10081 gaatccacgg tgacagaaga acgacgtcga ttattgatgg agattatatt ccacaaatgc
    10141 gaatttgtcg gagaaatggc tgttgtgcaa caggcacaac gtaatctctg tctggaaagt
    10201 tatgaccgta tagaacaaac gttaaaacat tgtattgaag cgaaaatgtt gcctgcggat
    10261 ttaatgacgc gtcgggcagc aattattatg cgcggctata tttccggcct gatggaaaac
    10321 tggctctttg ccccgcaatc ttttgatctt aaaaaagaag cccgcgatta cgtcgccatc
    10381 ttactggaga tgtatctcct gtgccccacg cttcgtaatc ctgccactaa cgaataaccc
    10441 tgaatctgac tccaggattt ttcctggaca ttttcgtcgt tgctattctg gttcactgcg
    10501 tcgtgatatt cttgcggttt gactttttca ggtcgttctt caggttcaga aaccttcatt
    10561 catcatgact atgttccagt attacaaacg atcacggcat tttgtttttt cagcatttat
    10621 tgcttttgtt tttgtcttgt tatgccagaa cacggcgttt gcgcgggcgt catcgaatgg
    10681 tgatctgccg acaaaagcgg acctgcaggc gcaacttgac tcactaaata aacaaaaaga
    10741 tctttctgct caggacaaac tggtgcagca ggatctgaca gatacattag ccaccctcga
    10801 taaaatcgat cgcataaaag aagagacagt tcagctacgg caaaaagtcg ctgaagcgcc
    10861 ggaaaaaatg cgccaggcga ccgcggcgtt aacagcactt agcgatgtcg ataacgacga
    10921 agaaacgcgc aaaattctga gtacgctttc gttgcgccag ctggaaactc gcgttgccca
    10981 ggcgctggac gatttgcaaa acgcacaaaa cgatctggcg tcttataaca gccagctggt
    11041 ttcgttacag acgcagcccg aacgcgtgca aaatgcgatg tataacgctt cgcagcagct
    11101 gcaacaaatt cgcagtcgtc tggatgggac tgatgtcggc gagactgcct tacgtcccag
    11161 ccagaaagtg ttaatgcagg ctcagcaggc gttgctgaat gcggagattg accagcagcg
    11221 taaaagcctg gaagggaaca ccgtcttgca ggataccttg caaaagcaac gtgattacgt
    11281 gacggcgaac agcgcccgtc tggagcacca gttgcagctg ttgcaagaag cggtaaacag
    11341 caagcgcctg actttaactg aaaaaacggc gcaggaagcc gtctccccgg atgaagccgc
    11401 gcgtattcag gctaatccgc tggtgaaaca ggaactggaa attaaccagc agttaagtca
    11461 gcgtctgatt accgcgactg aaaacggtaa tcagttgatg cagcaaaaca ttaaagtcaa
    11521 aaactggctg gagcgggcgc tgcaatcgga acgcaatatt aaagagcaga ttgccgtcct
    11581 gaagggcagc ctgctgttgt ctcgtatcct ttaccagcaa caacaaacgc tgccctcggc
    11641 ggatgaactg gaaaacatga ccaaccgcat cgcggatttg cgtctcgaac agtttgaagt
    11701 taaccagcag cgtgatgcac tcttccagag cgatgcgttc gtcaacaaac tggaagaagg
    11761 tcacaccaac gaagtcaaca gcgaagttca cgatgcgtta ttgcaagtgg ttgatatgcg
    11821 tcgcgaattg ctggatcaac tcaacaaaca gttgggtaac cagctgatga tggccattaa
    11881 cctgcaaatc aaccagcagc agttaatgag cgtatcgaaa aacctgaaat ccatcctgac
    11941 tcagcaaatc ttttgggtga acagtaaccg tccaatggac tgggactgga tcaaagcgtt
    12001 cccgcaaagc ctgaaagatg aatttaagtc gatgaaaatc acggtgaact gggaaaaagc
    12061 ctggcctgcc gtttttatcg ctttcctcgc cggtttgccg ctgctgttga ttgccgggct
    12121 gatccactgg cgtctgggct ggctgaaagc gtatcaacag aaactggctt ccgctgtggg
    12181 ttccctgcgt aacgatagcc agctcaacac accaaaagcg atccttatcg acctgatccg
    12241 cgcgctgccg gtgtgcctga ttattctcgc ggttggcctg attctgttga ccatgcagct
    12301 caacatcagc gaactgctat ggtcgttcag taaaaaactg gcgattttct ggctggtgtt
    12361 tggcctgtgc tggaaggtac tggagaaaaa tggcgttgcc gtgcgtcact tcggcatgcc
    12421 ggagcagcag accagccact ggcgtcggca aattgtccgc atcagtctcg cgttgctgcc
    12481 tatccatttc tggtctgtgg tggcagaact ttccccgctg catctgatgg atgatgtgct
    12541 ggggcaggcg atgattttct tcaacctgct gctgattgcc ttcctggtat ggccaatgtg
    12601 ccgcgaaagc tggcgtgata aagagtcgca caccatgcga ctagtcacca ttaccgtgct
    12661 gtcgattatc ccgattgcat tgatggtgct gactgccacg ggttatttct acaccacgct
    12721 gcgcctctcc gggcgttgga ttgaaaccgt ttatctggtg atcatctgga acttgctcta
    12781 ccagacggta ttgcgcggct taagcgtagc ggcgcggcgt atcgcctggc gtcgtgcgct
    12841 ggcgcgtcgg cagaatctgg tgaaagaggg cgcagaaggt gccgaaccgc cggaagaacc
    12901 caccattgca ctggagcaag ttaaccagca gacgctgcgt attaccatgt tgctgatgtt
    12961 tgcgctgttc ggtgtcatgt tctgggcaat ttggtccgat ttgatcaccg tgttcagcta
    13021 tctcgacagc atcacgctct ggcattacaa cggcactgaa gctggcgctg cggtggtgaa
    13081 aaacgtcacc atgggcagtc tgttgtttgc gattatcgcc tcaatggtgg cctgggcgtt
    13141 gattcgcaac ctgcctggtt tactggaagt gctggtgctc tcgcgactga atatgcgcca
    13201 gggcgcgtcg tatgccatta ctaccatcct taactacatc attattgccg ttggtgcgat
    13261 gacggtgttc ggatcgctgg gcgtctcttg ggataaactc cagtggctgg ccgcagcatt
    13321 atccgtaggt cttggttttg gtttacaaga aattttcggt aacttcgtct ccggtttgat
    13381 cattctgttc gaacgtccgg tgcgtattgg cgatacggta accattggta gcttctcggg
    13441 gacggtaagt aagatccgta ttcgtgcgac aacgattacc gatttcgatc gcaaagaagt
    13501 gatcatcccg aacaaagcgt ttgttaccga gcgtctgatc aactggtcgt tgactgacac
    13561 tactacgcgt ctggtgatcc gtctcggcgt ggcctatggc tccgatctgg aaaaagtgcg
    13621 taaagtgtta ctgaaggcgg cgactgagca cccaagggtg atgcacgaac caatgccgga
    13681 agtcttcttt acggcatttg gtgccagcac gttggatcat gagctgcgtc tgtatgtgcg
    13741 tgaactgcgt gaccgtagcc gtactgtcga tgagctgaac cgtactatcg atcagctgtg
    13801 ccgtgaaaac gacatcaaca ttgcctttaa ccagcttgaa gtgcatctgc acaacgagaa
    13861 gggcgatgag gtgacggaag taaaacgcga ctacaaaggc gacgatccga caccagcggt
    13921 tgggtaataa ccaaaggggg cgacatttag ttgccccgag attactgaca aagtgcgcgt
    13981 tgttcatgcc ggatgcggcg tgaccgcctt atccggccta cgaaaccgca agaattcaat
    14041 atattgcagg agcggtgtag gcctgataag cgtagcgcat caggcagttt tgcgtttgcc
    14101 cgcaatctta ggggacattt agcggcccca tttatttctc acttttccgc ctcatcatcg
    14161 tgcgttaatt tcttttcata atcacgcttt acaatatcca gcgcgcgcag cacggtactg
    14221 gcagggatct gattttcctc cagcagcaca atcaaatcga cagccagttt gacatcgtca
    14281 ggggcatttt caagtgacat actctttcca ttgctagcgg gttaagcgcg ctagcctgtt
    14341 ttcgattttt tccagcgcat ggcggcagcg tgccaggcgc gcttcatagg cttccacttc
    14401 acgatgcagc gtttgctgtt ccacgagatc ggtcacccgc gccaggcggg ctctgcgttc
    14461 ggcaaccatc tcacgtagcc gccgctcaaa atcctgatgc tgaatacgtt tacgctgcca
    14521 gcgggaaatt ttcggtggcg cactgtccca ctcgcgcaat gaccaggcgg aggcttcacg
    14581 cgcgatggct tccagttgtg ccgccagatg ttccgccagc caggccactt gcggcagctg
    14641 ttgctgctca actgcatgac gaagcgcagc cagattatcg cccgcctcgt cgagacaaga
    14701 ctgtagtgtt gtcgcacgag tctgaaaaag atgcctgtcg aaacgagcac ttagcgtggc
    14761 gaactgtgac accggggcac aacgctgacg cagcgtagcg agctgtcctt ccagtttttc
    14821 cagcagcagg gcggttttca cgataagacc tcaatgaaaa tgataattgt tatgctaaag
    14881 tagccactat gtaagctgac aacactcaat atgcaacgaa tcattttaat catcattggc
    14941 tggctggcgg tagtgctggg tacgctgggc gtggtattac cggtattacc gacgacgccg
    15001 tttatcctgc tggcggcctg gtgctttgcc cgttcttccc cgcgctttca cgcctggttg
    15061 ctgtaccgct catggtttgg cagctatcta cgtttctggc agaaacatca tgcgatgccg
    15121 cgcggcgtca aaccgcgggc gattttgctt attttgctca cgtttgccat ttctctgtgg
    15181 ttcgtccaga tgccatgggt gcgcatcatg ttgctggtaa ttctcgcctg tttgcttttc
    15241 tatatgtggc gaattccggt gattgatgaa aagcaagaaa agcactgaag cacaacaatc
    15301 gcagttgcaa ttattgcgta cagccagtac attctggcgt tttcgagcac aggcgcaggc
    15361 ggtcaaaggt taaacaacag ttacttttga tacgtttaaa acgcgccgtg agtaccaccg
    15421 taacaagcag gcatacactt atgaccgcga ctgcacagca gcttgagtat ctcaaaaata
    15481 gcatcaaaag cattcaggac tacccaaaac ccggcattct tttccgcgat gtcaccagct
    15541 tactggaaga cccgaaagct tacgctctca gcatcgactt gctggttgag cgttacaaaa
    15601 atgcgggcat taccaaagtt gtcggcaccg aagcgcgtgg cttcttgttt ggcgctccgg
    15661 tagctctggg tctgggcgtt ggctttgtac cggtccgtaa accgggcaaa ctgccgcgtg
    15721 aaaccatcag tgaaaattac gacctggaat acggcaccga tcagctggaa atccacgttg
    15781 atgccatcaa accgggcgac aaagttctgg tggtggacga tctgctggca accggcggca
    15841 ctatcgaagc gaccgttaaa ctgatccgtc gtctgggtgg tgaagtggct gacgctgcgt
    15901 tcattatcaa cctgtttgat ctcggcggcg aacagcgtct cgaaaaacag ggcattacca
    15961 gctacagcct tgtcccgttc ccgggccatt aattatcgcc agtcttgtgc tgcccacgct
    16021 acggacagca caagatgtgc attcagcctc gccgttctga cggggctgtg ttagcattac
    16081 cccttcgtga atccaccttc cagcgtttca gagcctgcca atgagttatc aggttttagc
    16141 ccgaaaatgg cgcccacaaa cctttgctga cgtcgtcggc caggaacatg tgctgaccgc
    16201 actggcgaac ggcttgtcgt tagggcgtat tcatcatgct tatctttttt ccggcacccg
    16261 tggcgtcgga aaaacctcta tcgcccgact gctggcgaag gggctaaact gcgaaaccgg
    16321 cattaccgcg acgccgtgcg gcgtgtgcga taactgtcgt gaaatcgagc aggggcgctt
    16381 tgtcgatctg attgaaatcg acgccgcctc gcgcaccaaa gttgaagata cccgcgacct
    16441 gctggataac gtccagtacg ctccggcgcg tggtcgtttc aaagtttatc tgatcgacga
    16501 agtgcatatg ctgtcgcgcc acagctttaa cgcactgtta aaaacccttg aagagccgcc
    16561 ggagcacgtt aagtttctgc tggcgacgac cgatccacag aaattgccgg tgacgatttt
    16621 gtcacgctgt ctgcaatttc atctcaaggc gctggatgtc gagcaaattc gccatcagct
    16681 tgagcacatc ctcaatgaag aacatatcgc tcacgagccg cgggcgctgc aattgttggc
    16741 gcgcgccgct gaaggcagcc tgcgagatgc cttaagtctg accgaccagg cgattgccag
    16801 cggtgacggc caggtttcaa cccaggcggt cagtgcgatg ctgggtacgc ttgacgacga
    16861 tcaggcgctg tcgctggttg aagcgatggt cgagggcaac ggcgagcgcg taatggcact
    16921 gattaatgaa gccgctgccc gtggtatcga gtgggaagcg ttgctggtgg aaatgctcgg
    16981 cttgttgcat cgtattgcga tggtacaact ttcgcctgct gcacttggca acgacatggc
    17041 cgccatcgag ctgcggatgc gtgaactggc gcgcaccata ccgccgacgg atattcagct
    17101 ttactatcag acgctgttga ttggtcgcaa agaattaccg tatgcgccgg accgccgcat
    17161 gggcgttgag atgacgctgc tgcgcgcgct ggcgttccat ccgcgtatgc cgctgcctga
    17221 gccagaagtg ccacgccagt cctttgcacc cgtcgcgcca acggcagtaa tgacgccaac
    17281 cctggtgccg ccgcaatcag cgccgcagca ggcaccgact gtaccgctcc cggaaaccac
    17341 cagccaggtg ctggcggcgc gccagcagtt gcagcgcgtg cagggagcaa ccaaagcaaa
    17401 aaagagtgaa ccggcagccg ctacccgcgc gcggccggtg aataacgctg cgctggaaag
    17461 actggcttcg gtcaccgatc gcgttcaggc gcgtccggtg ccatcggcgc tggaaaaagc
    17521 gccagccaaa aaagaagcgt atcgctggaa ggcgaccact ccggtgatgc agcaaaaaga
    17581 agtggtcgcc acgccgaagg cgctgaaaaa agcgctggaa catgaaaaaa cgccggaact
    17641 ggcggtgaag ctagcggcag aagccattga gcgcgacccg tgggcggcac aggtgagcca
    17701 actttcgcta ccaaaactgg tcgaacaggt ggcgttaaat gcctggaaag aggagagcga
    17761 caacgcagta tgtctgcatt tgcgctcctc tcagcggcat ttgaacaacc gcggtgcaca
    17821 gcaaaaactg gctgaagcgt tgagcatgtt aaaaggttca acggttgaac tgactatcgt
    17881 tgaagatgat aatcccgcgg tgcgtacgcc gctggagtgg cgtcaggcga tatacgaaga
    17941 aaaacttgcg caggcgcgcg agtccattat tgcggataat aatattcaga ccctgcgtcg
    18001 gtttttcgat gcggagctgg atgaagaaag tatccgcccc atttgatcgt aagcacagct
    18061 tacgttcgtc atccttaacg tgattgagag agaaacctat gtttggtaaa ggcggtctgg
    18121 gtaacctgat gaagcaagcc cagcagatgc aagaaaaaat gcagaaaatg caggaagaga
    18181 tcgcgcagct ggaagttacc ggcgaatctg gcgcaggtct ggtaaaagtg accatcaacg
    18241 gtgcacacaa ctgccgtcgc gtagagatcg acccgagcct gctggaagac gacaaagaga
    18301 tgctggaaga cctggtggct gcagcattca acgacgcagc acgtcgtatt gaagaaacgc
    18361 agaaagaaaa aatggcctct gtatcctccg gaatgcagct gccgcctggc tttaagatgc
    18421 cgttctgatg caaaccagcc cgctgttaac acagcttatg gaagcactgc gctgtctgcc
    18481 gggcgttggc ccgaagtcgg cgcagcgtat ggcgttcacg ctgcttcagc gcgatcgtag
    18541 cggcgggatg cgtctggcgc aggcgctcac ccgggcgatg tcggaaatcg gccactgcgc
    18601 cgattgccgc actttcaccg aacaggaagt ctgtaacatc tgttcgaatc cgcgtcgtca
    18661 ggaaaacggt caaatctgtg tggtggagag tccggcggac atctacgcca ttgagcagac
    18721 ggggcagttt tctggtcgtt attttgtgtt gatggggcat ctgtcaccgc tggacggcat
    18781 cggtccggat gatatcgggc ttgatcgtct ggaacagcgt ctggcagagg aaaaaatcac
    18841 tgaagtgatc ctcgccacca atcccacggt tgaaggtgaa gctaccgcta actacattgc
    18901 cgagctttgc gcgcaatatg acgtggaagc cagccgaatc gctcatggcg taccggttgg
    18961 cggcgaactg gaaatggtcg atggcaccac gctgtcacac tccctcgccg ggcgtcataa
    19021 gattcgtttt taagcaaacg agagcaggag cacctgctct cgcttgaaat tattctccct
    19081 tgtccccatc tctccctcat cctgttttta accttaaaat ggcattattg aggtagacct
    19141 acatgaaagg acaagaaact cgtggttttc agtcagaagt gaaacagctt ctgcacctga
    19201 tgatccattc tctctattcc aataaagaaa tcttcctgcg tgagcttatc tctaacgcct
    19261 ccgatgcggc ggacaagctg cgtttccgtg cgctatctaa cccggacctg tacgaaggtg
    19321 atggcgaact gcgcgttcgt gtctctttcg ataaagacaa gcgcacgctg accatctccg
    19381 ataacggcgt ggggatgacc cgcgacgaag tgattgacca tctggggact atcgctaaat
    19441 ccggtaccaa atcattcctc gaatccctgg gttctgacca agcgaaagac agccagctga
    19501 tcggtcagtt tggtgttggt ttctactctg cgtttatcgt ggccgacaaa gtgaccgtgc
    19561 gtactcgcgc ggcaggcgaa aaaccagaaa acggcgtctt ctgggaatcg gctggcgaag
    19621 gtgaatatac cgttgccgat atcaccaaag aagatcgtgg tactgaaatc accctgcatc
    19681 tgcgtgaagg cgaagacgag ttcctcgatg actggcgcgt gcgttccatc atcagcaaat
    19741 actccgacca tatcgcgctg ccggtagaga tcgaaaaacg cgaagagaaa gatggcgaaa
    19801 ccgttatctc ctgggcaaaa atcaacaaag cgcaggcgct gtggactcgt aacaagtcgg
    19861 aaatcaccga tgaagagtat aaagagttct acaaacacat cgcccacgac tttaacgatc
    19921 cgctgacctg gagccacaac cgcgttgaag gtaagcagga gtacaccagc ctgctgtaca
    19981 tcccgtccca ggctccgtgg gatatgtgga accgtgatca taaacacggc ctgaaactgt
    20041 acgttcaacg cgtgtttatc atggacgacg ctgaacagtt catgccgaac tatctgcgct
    20101 tcgtgcgtgg tctgattgac tccagcgatc tgccgctgaa cgtttcccgt gaaatcctcc
    20161 aggacagcac ggtaacgcgt aacctgcgca atgcgctgac caagcgtgtg ctgcaaatgc
    20221 tggaaaaact ggcgaaagac gacgcggaaa aataccagac cttctggcaa cagtttggcc
    20281 tggtactgaa agaaggtccg gcggaagatt tcgctaacca ggaagcgatc gccaaactgc
    20341 tgcgttttgc ttctacttat accgattctt ctgcgcagac cgtgtctctg gaagactacg
    20401 tttcccgcat gaaagaaggg caggagaaaa tctactacat cactgcagac agctatgcgg
    20461 cagcgaagag cagcccgcac ctggaactgc tgcgtaagaa aggcatcgaa gttctgctgc
    20521 tttccgaccg catcgatgag tggatgatga actatctgac tgagttcgac ggtaaaccgt
    20581 tccagtcggt gtctaaagtt gacgagtcgc tggaaaaact ggctgacgaa gttgatgaga
    20641 gcgcgaaaga agcggagaaa gcactgactc cgttcatcga ccgcgtgaaa gctctgctcg
    20701 gcgagcgcgt gaaagatgtc cgtctgactc accgtctgac cgatacgcca gcgatcgttt
    20761 cgaccgacgc ggacgaaatg agcactcaga tggcgaaact gttcgctgcg gcgggccaga
    20821 aagtgccaga agtgaaatac atcttcgaac tgaacccgga tcacgtactg gtgaaacgtg
    20881 cggcagatac tgaagatgaa gccaagttca gcgagtgggt agaactgctg ctggatcagg
    20941 cgctgctggc agaacgcggc acgctggaag atccgaacct gtttattcgt cgtatgaatc
    21001 agctgctggt ttcctgatgt catgccggat gacctttgtg tcatccggca tttttctttt
    21061 catcatctgt actttccgca aattatctcg ccattaaccg tttcagcctc aggtgccttt
    21121 cttgaggcaa tcgcctgttg gtggtatcgt ttatcgcttt ttcaaaaaaa tcgacacatt
    21181 ttaaggggat tttcgcaatg cgtatcattc tgcttggcgc tccgggcgcg gggaaaggga
    21241 ctcaggctca gttcatcatg gagaaatatg gtattccgca aatctccact ggcgatatgc
    21301 tgcgtgctgc ggtcaaatct ggctccgagc tgggtaaaca agcaaaagac attatggatg
    21361 ctggcaaact ggtcaccgac gaactggtga tcgcgctggt taaagagcgc attgctcagg
    21421 aagactgccg taatggtttc ctgttggacg gcttcccgcg taccattccg caggcagacg
    21481 cgatgaaaga agcgggcatc aatgttgatt acgttctgga attcgacgta ccggacgaac
    21541 tgattgttga tcgtatcgta ggccgccgcg ttcatgcgcc gtctggtcgt gtttatcacg
    21601 ttaaattcaa tccgccgaaa gtagaaggca aagacgacgt taccggtgaa gaactgacta
    21661 cccgtaaaga cgatcaggaa gaaaccgtgc gtaaacgtct ggttgaatac catcagatga
    21721 ctgcaccgct gatcggctac tactccaaag aagcggaagc gggtaacacc aaatacgcga
    21781 aagttgacgg taccaagcag gttgctgaag ttcgcgctga tctggaaaaa atcctcggct
    21841 aatcaagccg cagcagatct gctttcgggc aggtctgctt atccacctca ggcaatacct
    21901 ctcacagcaa ttagttcttc ttcctcactt ttccgctaca attatcaaca agttgaatcg
    21961 ataagaggcg gtaatgcgtc agactaaaac cggtatcctg ctggcaaacc tgggtacgcc
    22021 cgatgccccc acacctgaag cggtaaaacg ctatctgaaa caatttttaa gcgacagacg
    22081 cgtggttgat acctcacggt tgttatggtg gccgttgctg cgcggcgtga ttttgccgct
    22141 gcgctcgccg cgtgtggcga agctgtacgc ctctgtctgg atggaagacg gctcgccgct
    22201 gatggtttac agccgccagc aacagcaggc gttggcacaa cgtttaccgg atacgccagt
    22261 ggcgctgggc atgagctacg gctcgccatc actggaaagc tccgtcgatg aactcctggc
    22321 agagcatgta gatcatattg tggtgctgcc gctttatccg caattctcct gttctacggt
    22381 cggtgcggta tgggatgaac tggcacgcat tctggcgcgc aaacgtagca ttccggggat
    22441 atcgtttatt cgtgattacg ccgataacca cgattacatt aatgcactgg cgaacagcgt
    22501 acgcgcttct tttgccaaac atggcgaacc ggatctgctg ctgctctctt atcatggcat
    22561 tccccagcgt tatgcagatg aaggcgatga ttacccgcaa cgttgccgca caacgactcg
    22621 cgaactggct tccgcactgg ggatggtacc ggaaaaagtg atgatgacct ttcagtcgcg
    22681 ctttggccgg gaaccctggc tgatgcctta taccgacgaa acgctgaaaa tgctcggaga
    22741 aaaaggcgta ggtcatatac aggtgatgtg cccgggcttt gctgcggatt gtctggagac
    22801 gctggaagag attgccgagc aaaaccgtga ggtcttcctc ggtgcgggcg ggaaaaaata
    22861 tgaatatatt ccagcgctta atgccacgcc ggaacatatc gaaatgatgg ctaatcttgt
    22921 tgccgcgtat cgctaaagct gagcggtaaa gaactgagcg ccgtcgcgaa gagcctcgtc
    22981 ggcggttttc atcatccgtg aataatgcaa aaaggcatgc agcgtacctg ggtagagttt
    23041 gaactcacag ggctgctgat gcgccgctaa cgtctagtaa agcagacggc tgtcatccag
    23101 cagcggatcg aactccgccc cggcaataaa acagggcgga acttcgcgag tgagatcatt
    23161 attaaacaga cagtaatacg gtgactcgcg gtccgcgtcg ttgcttaaat acgcctcttc
    23221 atacatctgc aaatcctgtt gcgttaagcc atcccagaca ccgcccaaca gacgacgagt
    23281 cacggaatcc cgtaatccgt aaagcccata ccacagcaaa acacccgcaa ttttaccgca
    23341 atcgatctgt ttatcacgca accacaacgc actggcgagc gccagcatgg cacctgcgga
    23401 atcaccggca aagccaatgc gggacatatt gatttgataa tcctccgcct gctggtggaa
    23461 ataacaacaa gcagccacaa tttcctctat cgcttgtgga aaacgcgctt caggtgaaag
    23521 ggtgtaatta ataccaatca ccgtacattg gctgtagctt gccagcaggc gcatgatgcg
    23581 atcgtgggta tcgagattgc cgagaataaa accgcctcca tgcaaataaa atagcgtcgc
    23641 cgggctatct ggctgcggac aaaagagacg tgtttccacc tgcccatatt ttgttggaac
    23701 catgtaagct ctggttgcca tttctggagc gcccgcattc cagaatcggc gctcaagcgt
    23761 gtaatactgt cgttgctcag caatcgttcc cgttgcgggc caggacggta aatccggctg
    23821 aagagtattc acaacggtct tcatttcagc agaaataagg tccagaacag gtagtttgtt
    23881 ttccggcttc atacaaaaac tcctttcaaa ttacgtcatt gtaaggaacc actgccatga
    23941 aaatgcgatc ccgcctgctg atattgaaac tggctgcgtc tcgcgcgctc ccgtcagatt
    24001 gtgttaacat tcgccgctca gttaaccacc cgtaaaaaca accatgaaat ttcccggtaa
    24061 acgtaaatcc aaacattact tccctgtaaa tgcacgcgat ccgctgcttc agcaattcca
    24121 gccagaaaac gaaaccagcg ccgcctgggt cgtaggtatt gaccagacgc tggtcgatat
    24181 tgaagcgaaa gtggatgatg aattcattga gcgttatgga ttaagcgccg ggcattcact
    24241 ggtgattgag gatgacgtag ccgaagtgct ttatcaggag ctaaaacaga aaaacctgat
    24301 tacccatcag tttgcgggtg gcaccattgg taacaccatg cacaactact cggtgctcgc
    24361 ggacgaccgt tcggtgctgc tgggcgtcat gtgcagcaat attgaaattg gcagttatgc
    24421 ctatcgttac ctgtgtaaca cttccagccg taccgatctt aactatctac aaggcgtgga
    24481 tggtccgatt ggtcgttgct ttacgctgat tggcgagtcc ggggaacgta cctttgctat
    24541 cagtccaggc cacatgaacc agctgcgggc tgaaagcatt ccggaagatg tgattgccgg
    24601 agcctcggca ctggttctca cctcatatct ggtgcgttgc aagccgggtg aacccatgcc
    24661 ggaagcaacc atgaaagcca ttgagtacgc gaagaaatat aacgtaccgg tggtgctgac
    24721 gctgggcacc aagtttgtca ttgccgagaa tccgcagtgg tggcagcaat tcctcaaaga
    24781 ccacgtttct atccttgcga tgaacgaaga tgaagccgaa gcgttgaccg gagaaagcga
    24841 tccgttgttg gcatctgaca aggcgctgga ctgggtagag ctggtgctgt gcaccgccgg
    24901 gccaatcggc ttgtatatgg cgggctttac cgaagacgaa gcgaaacgta aaacccagca
    24961 tccgctgctg ccgggcgcta tagcggaatt caaccagtat gagtttagcc gcgccatgcg
    25021 ccacaaggat tgccagaatc cgctgcgtgt ctattcgcac attgcgccgt acatgggcgg
    25081 gccggaaaaa atcatgaaca ctaatggagc gggggatggc gcattggcag cgttgctgca
    25141 tgacattacc gccaacagct accatcgtag caacgtacca aactccagca aacataaatt
    25201 cacctggtta acttattcat cgttagcgca ggtgtgtaaa tatgctaacc gtgtgagcta
    25261 tcaggtactg aaccagcatt cacctcgttt aacgcgcggc ttgccggagc gtgaagacag
    25321 ccaggaagag tcttactggg atcgttaagt tatcgtcggt tcgtaggcca gataaggcgt
    25381 tcacgccgca tctggcattt ggctctcgat gcctgatgcg acgctggcgc gtcttatcat
    25441 gcctacatat ttttcatatt ttacatccgg caaccaccgt ttaccccgtc accacttcac
    25501 ccgccggtgg cgtttccagc agttccagca tagtacgggc aatttcacgt tcacccatca
    25561 ctacctgatt cgcgccacgt tcggtgatat acgtcacttc atcgtcataa tgggcgcggg
    25621 cgataatctc aatatccggg tttttcgcgc gggcagacgc cacaatctca cccgcttcat
    25681 aaccgttggg gatcgtcagg atcagccatt ttgcacattc cagatgtgcc agttgcataa
    25741 tttcttcgtt cgctgcattg cccaatacag cgcggacccc acgctctcgc agctcatcaa
    25801 cacgggttcg tgacgtctca atcaccacca gcggaatatc agaggcgagc aatttctccc
    25861 ccagcaggct gcctacacga ccgtaaccca ccaatagcgc atggttgcaa atatccactg
    25921 ggatctgttt ctcttcttcg attgcctctt ccagcgtctg ctcttccagc gtttcggtct
    25981 tcgccagata tttctccagt agtgcgaaca gtaccgggtt gagcataatc gacaggatcg
    26041 cccctgccag taccaggttt tgtccggcct gcggcagtaa attcaatgcc attcccagtc
    26101 ccgccaggat aaacgcgaac tcaccaatct gcgccaggct ggcggcgatg gttaatgccg
    26161 tacgttggga gtgaccaaac agacggacaa ggaacagggc cgcgaccgat ttaccaaaca
    26221 gaataattgc cagcgtcgcc agcactgcca gcggttgctg aatcagaatt aacggatcaa
    26281 acaacatccc gactgagaca aaaaacagca ccgcaaacgc gtcgcgcaat ggcaacgtat
    26341 cgtgggcggc acggtgactc aattcagact cgttcagtac catcccggca aagaacgcac
    26401 cgagtgcaaa ggagacatca aacagctcta ccgcaccaaa ggcaatccct aacgccagcg
    26461 ccagcactga cagggtaaac agctcgcgag aaccggttgc cgcgctgcgt gccataatcc
    26521 acggcaccag acggcgacct accagcatca taatggcgat aaatgcgatc accttgccga
    26581 tggtgatccc catatcgact gcaagagtgg caaagcccac atcgccctgt tccatcattc
    26641 ctgccactgc gggcagcaac accagcgtca gaaccatcac caggtcttcc acaatcaacc
    26701 aaccgatggc gatttgccca cgctgactgt ctattaattg ccgttcttca agtgcgcgca
    26761 gtaacaccac ggtactggcg gtggaaagac ataagccgaa cacgataccg gtcattaacg
    26821 accagcccag cacggcggag agcgccatac ccagcagcgt cgccacggct atctgggcga
    26881 tcgcgccggg aatggcgatg gactttaccg ccatcaaatc cttgagcgaa aaatgcaaac
    26941 cgacgccgaa catcagcaaa ataacgccca gttcagccag ttccggcgca agcttggtat
    27001 cggcaacaaa gcccggagtg aatggtcctg ccagcacacc cgctaacaga tatcccacca
    27061 gaggagaaat acgtagttta ttggccagca tgccgaggat aaaggcgagc acaaggccgc
    27121 caacaatggt ggtgataagc ggggtggcgt gatgcattcc gtctcctttt cctggtggtt
    27181 attgtccatt tttggctggg aaaaccaaaa ttacaggtaa tagtttatga caatttcatt
    27241 gatgatgttt atgaataatt gttgaatttt gcagaaaaat ggaattagct gcaaaaaaag
    27301 cacggttcgg aaaacagaag gcggtttaac agaggaaagg cttatggcga ctgtaaaacg
    27361 atgcagccaa agtttgcctt tggctgcaat gaaatcagtc tttatgccgg ttatcaggca
    27421 ggaatatggt caacatcccc aatagtggca ggaaagcaca gattttatag actaactcga
    27481 tgctggtgtg atcggcgata agccccagaa ctgccgctcc cagacctccc atgccgaaag
    27541 caaaaccgaa aaagagtcca gaaaccatac cgatacgtcc cggaagtagc tcctgagcgt
    27601 agaccagaat ggcagagaat gccgaagcga ggataaatcc aataatcacc gttaaaaccc
    27661 ccgtccagtg caggcgggcg tagggtaaaa tcagcgtaaa cggcgcaacg ccgaggatag
    27721 agccccaaat cacatatttc cgtccaattt tatcccctac aggcccgccg atcaccgtac
    27781 ctgccgcaac ggcaaacagg aaggcaaaca gatgaagctg ggcattctgg atagataatc
    27841 cgaatttttg catcagataa aaggtgtaat agctgctgat gctcgccata tagaaatatt
    27901 tcgagaaaat gaggattaac agaatgctga ctgccagtac cactttgttg cgcggcagtg
    27961 gattgattat cgtcgctttg ggttttcctt tattcattcg gtgctgtgcc gagtaccaac
    28021 ggctaatttg cgccaacacc acgatcgcca gcagtgccgc aagcacaaac caggcaacgt
    28081 tgcctttgcc ataaggcgcg ataatcaccg ccgccagcaa gggtcccagg gaactgccaa
    28141 agttgccgcc gacctgaaag atagattgcg ccaggccatg ccgcccgccg gaagccatac
    28201 gggctacgcg agaagattcc ggatgaaaga ccgatgaacc ggtaccgacc agcgccgctg
    28261 ccagcagaac tgcgccaaaa ctgcccgcca gcgcaagcag caccagacca cttaaggtaa
    28321 agcacatgcc aattggcaac gaccacggca tcggatattt atcggtccag tagccgacca
    28381 ctggttgcag tagcgaagag gcgagctgga aggtgagggt tatcatgcca atctgcataa
    28441 atgtcagaga aaattctgac tgaagcagcg gataaatcgc cagaatcagc gattggatca
    28501 tgtcgttcag cagatgtgag aggctgatag cacctaaaat accaaacgat gttcgggcct
    28561 tggtcgttga cgcagccgcg cccgccacag gctgggtttg ttcactcatt gccataggaa
    28621 agtcactttt tcagggttgc gatgtaaaga atgatcttat ttgtgattat tacccgacta
    28681 acatacctgt atgcgtcgtc tgaaggaagt ctcaacgccg aatacagaat ttctaatctg
    28741 gatgcagatt tatcttcacc ggacgcagac ttgtctatga tgttgcgtca tactattttt
    28801 caacacgttg aaatcaggtc agggagagaa gtatgaaatt attgcagcgg ggcgtggcgt
    28861 tagcgctgtt aaccacattt acactggcga gtgaaactgc tctggcgtat gagcaggata
    28921 aaacctacaa aattacagtt ctgcatacca atgatcatca tgggcatttt tggcgcaatg
    28981 aatatggcga atatggtctg gcggcgcaaa aaacgctggt ggatggtatc cgcaaagagg
    29041 ttgcggctga aggcggtagc gtgctgctac tttccggtgg cgacattaac actggcgtgc
    29101 ccgagtctga cttacaggat gccgaacctg attttcgcgg tatgaatctg gtgggctatg
    29161 acgcgatggc gatcggtaat catgaatttg ataatccgct caccgtatta cgccagcagg
    29221 aaaagtgggc caagttcccg ttgctttccg cgaatatcta ccagaaaagt actggcgagc
    29281 gcctgtttaa accgtgggcg ctgtttaagc gtcaggatct gaaaattgcc gttattgggc
    29341 tgacaaccga tgacacagca aaaattggta acctggaata cttcactgat atcgaatttc
    29401 gtaagcccgc cgatgaagcg aagctggtga ttcaggagct gcaacagaca gaaaagccag
    29461 acattattat cgcggcgacc catatggggc attacgataa tggtgagcac ggctctaacg
    29521 caccgggcga tgtggagatg gcacgcgcgc tgcctgccgg atcgctggcg atgatcgtcg
    29581 gtggtcactc gcaagatccg gtctgcatgg cggcagaaaa caaaaaacag gtcgattacg
    29641 tgccgggtac gccatgcaaa ccagatcaac aaaacggcat ctggattgtg caggcgcatg
    29701 agtggggcaa atacgtggga cgggctgatt ttgagtttcg taatggcgaa atgaaaatgg
    29761 ttaactacca gctgattccg gtgaacctga agaagaaagt gacctgggaa gacgggaaaa
    29821 gcgagcgcgt gctttacact cctgaaatcg ctgaaaacca gcaaatgatc tcgctgttat
    29881 caccgttcca gaacaaaggc aaagcgcagc tggaagtgaa aataggcaaa accaatggtc
    29941 gtctggaagg cgatcgtgac aaagtgcgtt ttgtacagac caatatgggg cggttgattc
    30001 tggcagccca aatggatcgc actggtgccg actttgcggt gatgagcgga ggcggaattc
    30061 gtgattctat cgaagcaggc gatatcagct ataaaaacgt gctgaaagtg cagccattcg
    30121 gcaatgtggt ggtgtatgcc gacatgaccg gtaaagaggt gattgattac ctgaccgccg
    30181 tcgcgcagat gaagccagat tcaggtgcct acccgcaatt tgccaacgtt agctttgtgg
    30241 cgaaagacgg caaactgaac gaccttaaaa tcaaaggcga accggtcgat ccggcgaaaa
    30301 cttaccgtat ggcgacatta aacttcaatg ccaccggcgg tgatggatat ccgcgccttg
    30361 ataacaaacc gggctatgtg aataccggct ttattgatgc cgaagtgctg aaagcgtata
    30421 tccagaaaag ctcgccgctg gatgtgagtg tttatgaacc gaaaggtgag gtgagctggc
    30481 agtaatccga aagtgccgga tgtttgcatc cggcacaatg cttaatcgcg gcgggcgata
    30541 tcagcaaatt tggcatcgag gatctttgcc agatcgcctg ccgccagttc gatatccagt
    30601 ccgcgcttgc cgccggaaac ataaatagtg gcaaattctt gtgcgggggc gtcgataatc
    30661 gttggcagac gttttttctg ccccagtggg ctaatccccc caaccaggta tcccgtcgaa
    30721 cgctgcgcga ccatcggatc ggccatctca actttcttgg cacccagcgc ttttgctact
    30781 tttttaagat ccagttgacc tgcgaccggc gtaacggcca cggcaaggtg tttcatatca
    30841 ccgttcactg ccaccagcag cgttttgtag acctgatccg gattcaaacc taattttttg
    30901 acgacttcat cgccaaaatt ggtttcagcc ggatcgtgct cgtaggtatg gatttgaaac
    30961 gaaatcttgt ttttttcgag taatttaact gcgggtgtca tggtaatttt cctgccttaa
    31021 acaaaaaaac gaagcaagca tacgcctgat ggcggtccaa aaaaaagagt catcttgcct
    31081 aagagtattg gcaggatggt gagattgagc gacaatcgag ttacaccgtc gcttaaagtg
    31141 acggcataat aataaaaaaa tgaaattcct ctttgacggg ccaatagcga tattggccat
    31201 ttttttagcg caacatttgc ggcaaattcc cttctccata caggtgtagt gcaccgaccg
    31261 cgaccacata tcgccccggc ggcatggcgc gtaatttatc ccgccaggcg agatttcgct
    31321 gatgcatcag cacatcgtac agcgactgac tgaacgtatt gggcagcgtt atatcattat
    31381 tttgcggcgg tgcattcagc caccagctca tcatttgttg cagcaaccgt gcgttggtat
    31441 gccagtgggt cagcgtatcg tccagcagcg ccagtccttt gtcagggagc tggagcaaca
    31501 tggcaatctg gttttcagcc ccttccagtt caatcacggg tttatgttgt tgcttcgccg
    31561 cctgcaatag ctggtaatcg ataccgtatt ctgcccgcag ccccagtttt tgtgcctgcg
    31621 tcgcctgaag aaccatcgcg atttgccaca gcggttgggt agaaaagagt gagggagaaa
    31681 tgcccatctc ctggctaatg tgctgcaggt tttggagttg ctcctcgcta atgcgctctt
    31741 ccagcgcctc gcaggcaggc aaattagcaa aaggcgtatc gctggtggaa acatccgcct
    31801 cgacgatcag cgcatcggcg tttttgagct ttttgagcaa acgggtgggc aggggagcca
    31861 tatcgtggct acccatatga atactgccaa tcagatgaaa atggcgattg ccggggaggg
    31921 tgatatcgat ggcaggccag gtgtaatgat taccgcgcag cgcggcccaa agcgttttta
    31981 cccggtacaa cagatccata cgacctccct ttgtgaaata tcatgctagc gcgcggtgag
    32041 ggatggcgca aatggagttg gggacttgtt taatgtgttt gtatgattca gtatgttctt
    32101 gcatcgctat tcacagggaa gcaacagtta aaaaccatga aacaggcaac aagaaaaccg
    32161 acgacacccg gagatattct tctctatgaa tatctggaac cgctcgattt gaaaatcaat
    32221 gagttagcag agttgctgca tgttcatcgt aatcgcgtca gtgcactgat caataacaat
    32281 cgtaaactca ccactgagat ggcattccgt ctggcgaaag tttttgatac cacagtcgat
    32341 tttaggctaa acctccaggc ggcagttgat ctttgggaag ttgaaaacaa catgcgcacc
    32401 caggaagaat tgggacggat tgaaacagtg gctgaatatt tggcacgccg tgaagagcgt
    32461 gcaaaaaagg tcgcgtaagg ccaaaacatc gcttcatctc gtaggccgga taaggcgttc
    32521 acgccgcatc cggcatccga gcaccattgc ctgatgcgac gcttacgcgt cttatcaggc
    32581 ctacaaacct gtgcctgaac cgtaggccgg ataaggcgtt tacgccgcat ccggcagtca
    32641 tgcgtcgatg ccaaatgcgc cgccctaaag cagcgcatcc gcaatgacgt acttattcct
    32701 tcggtttaaa ccgcagcaac cggttggcgt tacttactac ggtaattgac gagagcgcca
    32761 ttgccgctcc ggcaactacc gggttaagca gtgttccagt aaacggccac aaaataccgg
    32821 cggcgaccgg aataccgatg ctgttgtaga taaaggcacc gagcaggttc tgcttcatgt
    32881 tgcgcagcgt tgcgcgggaa atcgcgagcg catccgcaac gcccatcagg ctatggcgca
    32941 tcagggtaat cgccgcggtt tcaatggcaa catcactgcc gccacccatc gcaatgccga
    33001 catccgcctg ggccagcgct ggcgcgtcgt taatgccgtc gcccaccatt gccacctgac
    33061 gtccttcact ttgcagacgt ttgatcgctt cggctttacc gtccggcagc actccggcaa
    33121 tcacttcatc aattccagct tctttggcga tcgcattggc ggtggttggg ttatccccgg
    33181 tcaacatcac cagacgatat cccgctttat gcaggcgttg cagcgccgcc acgctatcac
    33241 tacgcaacgg atcgcgtaca gccagcaggg ctactgcttt cccgtcaatc gccagcagca
    33301 caggcgttgc tccttgcgat gcctgagctg aaatatcggc ttcaatcgct ttagtatcga
    33361 cttgctgatc atttaacaac gcctgattac ccagcaataa cgcatgacct tctgcttcac
    33421 cgctcacgcc cagcccgcgc aatgtgcgga aaccgttgac ctgcggtagc tgcatatcgc
    33481 ctgctttatc aaggatcgct cgtgccaatg gatggctgga accttgctcc agcgccgccg
    33541 ccagacgcag cgcctgcgct tcgtcaacat cagcaaatgt tttcactgcg acaacctgcg
    33601 gcttcccttc ggtcagcgtc ccggttttat cgaacacgac ggtatcgagt gtactggcgc
    33661 gttgcagcgc gtcggcgtcc cgcaccagca cgccaaactc agccgcccgc ccgacgccgg
    33721 aaataatcga catcggcgtc gccagtccca gcgcacacgg gcaggcgata atcagcaccg
    33781 tagtggcaat caccagggta tagacaatct gcggtgccgg accaaagaaa taccagattg
    33841 ccgcactgac aagcgcaata accactacta ccggcacaaa tatggctgag attttatccg
    33901 ccagctgacc gatttctggc ttgctgctct gggcctggcg caccatgcga atgatccttg
    33961 atagcgtggt atggctgcca accgcactgg cacgaaacag cacactgccg tcctgtacca
    34021 ctgtcccggc atggacgcta tcgccttcgc ctttttgctg cgggattggt tcgcccgtca
    34081 gcatcgcttc atccagccat gcttcgccct gggtaatctc gccatctacc ggcacgcgat
    34141 cgccggtcgt caggcgcagc aacatacctg gctgcacttc tgccagaggc acgctttttt
    34201 caccttcgtc agtaaccagg cgcgccgtcg gtggggttaa atcgagtaat ttctccagtg
    34261 ccttagaaga acgctgacgt gcgcgtgctt ccagcatatg gccgagattg atcagaccga
    34321 taatcatcgc actggcttca tagtaaagat gtcgcgcttc catcgggaac cactgcggcc
    34381 acaggttgac gctcatcgaa tagagccacg ccacgcccgt tcccagcgcc accagcgtat
    34441 ccatcgtcgc tgcaccgttc agcaggcttt tccatgcact gcggtaaaaa tggccgctgg
    34501 cgaaaaccat caccgccagg gttatcaggc cgataaccaa ccacaggctg cggttgtcgg
    34561 cggtgactat catgttatcg ccgatcatcc cccagaccat caccgggata cccaccgcca
    34621 gtgcgacaat tgcctgccag cggaagcgct tcatcgtagc gacggcggtt tcttgctggc
    34681 gttcgcggcg tttcgcgtca tcttcaatcg cttccgcgcc gtagcccgct ttttccaccg
    34741 cctgcactaa atcttgtggg gaggcactgc ccatcaccag cgcagtacgc tccgccaggt
    34801 ttacccgtgc ctgagtgacg cccggtacgc tttgcagcgc attttgtacg cgggtgacac
    34861 agctggcgca gctcatgccg ctcagcagca actgctggct gtcatcgtca tcggcggtcg
    34921 ctgccggaag cgcctcagaa accgctgtca gtgcttccga cgggattgat gactccgcca
    34981 gcggtttagc ctttgggtgg cttacagatg cgtcataacc cgcttgtttg atggtttcga
    35041 tcagctgttc tgcactggca gtcccggtaa cgtgcgcttc agtgatagac acatccgctt
    35101 gttccacatc cgggcgctgt tcaagacttt ctttcacgcg tttaacgcag tgaccgcagg
    35161 acaggccgtc caggttcagg tcgatagttt gtgacataaa acactccttt aagacagttt
    35221 tgactggctg tgataaaggt taaaccttcc agcaagggga aggtcaagaa attaataaac
    35281 caggcgggta aaagtccgta aagattaaaa aatcggctcg atttgcatca ggattagaca
    35341 tttatttctt tgttttcctg tagttaagtt gcgggtgcta agttaaatca ggatgcctga
    35401 aaatcggcac tggggtgagg aattacctcc cgcatctata aaaaggagtt aacaaaagat
    35461 gttagatgca aacaaattac agcaggcagt ggatcaggct tacacccaat ttcactcact
    35521 taacggcgga caaaatgccg attacattcc ctttctggcg aatgtaccag gtcaactggc
    35581 ggcagtggct atcgtgacct gcgatggcaa cgtctatagt gcgggtgaca gtgattaccg
    35641 ctttgcactg gaatccatct ctaaagtctg tacgttagcc cttgcgttag aagatgtcgg
    35701 cccgcaggcg gtacaggaca aaattggcgc tgacccgacc ggattgccct ttaactcagt
    35761 tatcgcctta gagttgcatg gcggcaaacc gctttcgcca ctggtaaatg ctggcgctat
    35821 tgccaccacc agcctgatta acgctgaaaa cgccgaacaa cgctggcagc gaattttaca
    35881 tatccaacag caactggctg gtgagcaggt agcgctctct gacgaagtca accagtcgga
    35941 acaaacaacc aacttccata atcgggccat tgcctggctg ctgtactccg ccggatatct
    36001 ctattgtgat gcaatggaag cctgtgacgt gtacacccgt cagtgctcta cgctcatcaa
    36061 tactgttgaa ctggcaacgc ttggcgcgac gctggcggcg ggtggtgtga atccgttgac
    36121 gcataaacgc gttcttcagg ccgacaacgt gccgtacatt ctggccgaaa tgatgatgga
    36181 agggctgtat ggtcgctccg gtgactgggc gtatcgcgtt ggtttaccgg gcaaaagcgg
    36241 tgtaggtggc ggtattctgg cggtcgtccc tggcgtgatg ggaattgccg cgttctcacc
    36301 accgctggac gaagaaggca atagtgttcg cggtcaaaaa atggtggcat cggtcgctaa
    36361 gcaactcggc tataacgtgt ttaagggctg atcatgatga acacggaagg taataacggt
    36421 aacaaacccc tcggtctatg gaacgtcgtt tccatcggta ttggggcaat ggtgggggcg
    36481 gggatcttcg cgctgctggg gcaggctgcg ttgctaatgg aagcctcgac ctggggcgcc
    36541 tttgcttttg gcggtattgt ggctatgttt tccggttatg cctatgcgcg tctgggggcg
    36601 agctatccca gcaatggcgg tattatcgac ttttttcgtc gcggattagg caacggcgtc
    36661 ttttcgctag cgctctcgtt gctatacctg ttgacgctgg cggtgagcat cgccatggtt
    36721 gcccgtgctt ttggcgctta tgccgtgcag tttttgcatg aaggcagcca ggaggagcac
    36781 cttattttgc tctacgcgtt ggggatcatt gcggtgatga cgcttttcaa ctccttaagc
    36841 aaccatgcgg tagggcggct ggaagtgatc ctcgtcggca ttaaaatgat gatcctgtta
    36901 ttgctgatta ttgccggtgt ctggtcgctg caaccggcgc atatttccgt ctctgcgccc
    36961 cccagctccg gtgcgttctt ctcctgtatt gggataacct tccttgccta tgcgggcttt
    37021 ggcatgatgg cgaacgcggc ggataaagtg aaagatccgc aggtcattat gccacgggcg
    37081 tttctggtgg cgattggcgt taccacgttg ctttatatct cgctggcgct ggttttgctt
    37141 agcgatgtat cggcattaga gttagaaaaa tatgctgata ccgccgtagc gcaggctgct
    37201 tctccgctgc tcgggcatgt gggttatgtg atcgtcgtca tcggcgcttt actggcgacg
    37261 gcttcaggca ttaacgcgaa cctgttcgcc gtgtttaaca tcatggacaa catgggcagc
    37321 gaacgcgaac tgccgaagct aatgaataaa tccctgtggc ggcagagtac ctggggcaat
    37381 atcatcgtcg tggtgttgat aatgctgatg acggcggcgc taaatttaga ctcactcgcc
    37441 agcgttgcca gcgccacctt tttgatctgc tatctggcgg tgtttgtggt ggcgatccgc
    37501 ctgcgtcatg atattcacgc ctcgttgccg attcttatcg ttggtacgtt ggtgatgttg
    37561 ttggtgatcg ttggctttat ctacagcctg tggtcccagg gcagccgtgc gttgatatgg
    37621 attattggtg caatcttact cagccttatt gtggcaatgg tcatgaagcg taataaaacc
    37681 gtataacacc tctctgtgcg cagtacttcc tgtattattg tggtggcggt cgatattcgc
    37741 actggcaaaa aaacgtgctt gaatatctgt tgaaaccctt taacaaagca caggaggcgt
    37801 tgcgcgaacg atgaacatca gcgatgtagc aaaaattacc ggcctgacca gcaaagccat
    37861 tcgcttctat gaagagaagg ggctggtgac gccgccgatg cgcagcgaaa acggttatcg
    37921 cacctacacg cagcagcatc tcaacgaact gaccttactg cgccaggcac ggcaggtggg
    37981 ctttaacctg gaagagagcg gcgagctggt gaatctgttt aacgacccgc agcggcacag
    38041 cgccgacgtc aaacggcgca cgctggagaa ggtggcggag atcgaacgac acattgagga
    38101 gctgcaatcc atgcgcgacc agctgctggc actggcgaat gcctgccctg gcgatgacag
    38161 cgccgactgc ccgattatcg aaaatctctc cggctgctgt catcatcggg cagggtgatt
    38221 aagacgagac ggctcggata tgtagggtta tcccttctat cgcaatgact tcaacatgcg
    38281 tacctgcacc gagatcctcg ctggcgctga caggccatga actgtcaccg acgcgcatat
    38341 gaccgcgccc gttgaccagc ggagattcca gcacaaaacg tcggccaatc agctgctgcc
    38401 cgcgctggtt taaatgacta tcgctgtgct tttgttcgcg cacccgccgc gacaaccatt
    38461 tccaccacag ccaggcggcg agcagcgtca ggatggcaaa cattacccct tgccactccc
    38521 aacccagcgg caccagccag accaccaggc cggtaatcac cgctgccacg ccgctccaca
    38581 acaaataacc atttccgccc agcatctcgg ctgccagcag caacccgccg agactgagcc
    38641 agaaaatatg tggatgaacg accattaact ccatcatggc tgagtccgtt tgttggcgct
    38701 gtctttcacc agctcggcaa tcccggcaat cgaccccatc aggctgctgg cctctaatgg
    38761 catcatcact actttgctgt tactggagga accgatctgc tgtaacgctt cggtgtattt
    38821 ttgcgctacg aagtagttca ccgcctgaat atcaccggag gcgatagctt cagacaccat
    38881 tttggtggcg cgggcttctg cttcggcgga acgttcacgc gcttcagcct gtaaaaacgc
    38941 cgactgacgt tcgccttccg ctttcaggat ttgcgactgt ttttcacctt cggctttgag
    39001 gatttccgcc tgacggatcc cttccgcttc aagaatgtaa gcgcgtttgg tacgttccgc
    39061 tttcatctgc gcgttcattg aagagataag ctctgccggt gggcgcacgt cgcgaatttc
    39121 aatacgggtg actttaatcc cccacgggtt ggtggcttca tcgacaatac gcagcaggcg
    39181 tgagttgatg ctgtcgcgct gagagagcat ttcgtcaagt tccattgaac ccaacacggt
    39241 acggatgtta gtcatggtca ggttgatgat cgccagctcc agattgctga cttcataggc
    39301 agcgcgtggc gcgtcaatca cctgaataaa acagactgcg tcgatggtaa cgttggcgtt
    39361 atctttcgag ataacttcct gggaagggat atcgagcact tgctccatca tattgatctt
    39421 gcgaccaatg cgatccataa acggcaccac cagactgagc cccggctgta acgttttggt
    39481 atagcgacca aaacgttcta ccgtccattg atagccctgc ggcacgattt tgacgcccgc
    39541 gccgacaatg accagcgcga caaaaatgag aatcgggata aagataagca tcggaaaaac
    39601 ctcctgttgt accgtccata atcagcaaaa ttgctgcttg attaaacaaa ttatacctga
    39661 ttactgaaag agagttcccc cttattcctg cgaaggataa actgttttta gtaaaaatca
    39721 gaaaaaggga acagcgatgc aggaaaatag tcctttgctt cagctacaaa acgtaggata
    39781 tctggcgggt gatacgaaga ttcttaataa catcaatttt tcgctgcgtg ctggcgaatt
    39841 taagttaatt accggtcctt ctggttgtgg caaaagtacg ctgctaaaaa tagttgcttc
    39901 attgatcagc ccaaccagcg gaacgttact gtttgaaggt gaggatgtca gcacactaaa
    39961 accagaaatc taccgccagc aagtctctta ctgcgcccag acgccgacgc tgtttggcga
    40021 tacggtatac gataatctga tctttccctg gcagatccgt gaccagcagc ctgacccggc
    40081 catttttctc gattttctcg aacgcttcgc tttgccggac agcattttga cgaagaatat
    40141 cgccgagcta tctggtggtg aaaaacaacg catctcattg attcgtaacc tgcaatttat
    40201 gccgaaggtt ttattgctgg atgaaataac cagtgcgctg gatgaaagta ataaacataa
    40261 cgtcaatgag atgatccatc gttatgtgcg cgagcaaaat attgccgtgc tgtgggtgac
    40321 acacgataaa gacgaaatta atcatgcgga taaagtgatt acactgcaac cgcatgccgg
    40381 agaaatgcag gaagcacgct atgaactcgc ataatattac taacgaatca ttagcactgg
    40441 cattaatgct ggtggtggtg gcaatcttaa ttagccataa agaaaaactg gcgctggaga
    40501 aagatattct atggagcgtc gggcgagcga taattcagct gattattgtc ggctatgtgc
    40561 tgaagtatat tttcagcgtg gatgatgcca gcctgacatt attgatggtg ttatttatct
    40621 gctttaatgc ggcgtggaac gcgcaaaaac gcagtaaata tattgctaaa gcttttatct
    40681 catcgtttat tgccattacg gtcggggcgg gaattaccct ggcggtgctg attctctcag
    40741 gggcgattga atttatcccg atgcaggtga tccctgtcgc cgggatgatt gccggtaacg
    40801 ccatggtagc ggtggggttg tgttacaaca atttagggca acgggtcatt agcgaacagc
    40861 aacagattca ggagaaactg agtcttggcg cgacgccgaa gcaggcttca gcgatattga
    40921 ttcgcgacag tattcgcgcg gctttaattc cgacggtcga ttcagcaaaa acggttggct
    40981 tagtgagttt accaggaatg atgtccgggc tgatatttgc cgggattgat ccggtgaagg
    41041 cgattaaata tcagattatg gtgaccttta tgctgctctc aaccgccagc ttgtcgacca
    41101 ttattgcctg ctatttaacc tatcgtaagt tttataattc gcgccaccag ttggtggtga
    41161 ctcaattgaa gaagaaatga tgcgatgccg gatgcgcagc atcgcatccg gcgttgtggt
    41221 tcatgtgccg aacaacggaa tatcaataca acaatgcata cagctggcgg cgatacttcg
    41281 acgccagtgc atcacccgta cccagcgcag cgaggatctc ctggaacgtt ttacgcgtct
    41341 gaccgtctgc ggcggtgaga tctttacgca gatgcccgaa cagcaactcc agcgcctctt
    41401 cattgcgccc aacctgatgc agttgcagcg ccagttgcgt cgccagtgcg gcatcttctg
    41461 gattctccgc cacctgctgt tgcaactgtt gaatttccgg cttatcagcc gcctgcttca
    41521 gcagttcgat ttgcgctacc agcccctggt agcgggtgtc ctgatcctgc aacggaatgg
    41581 ttttcagcac cgcttccgca tcttcagaac ggttcagcgc aatcagcgtt tctgccagca
    41641 gcaggccgat ctccccgttc tgattcgaca actgccaggc gtctttcagc aatggcaggg
    41701 catcggtgta attgccttcc tgcatcagtt gcatcgcctg ctgcgctttc agctcttctt
    41761 cgcgcggcag cactttatcc agcagggcgc ggatcgcctc ttccggttgc ggcccctgga
    41821 agccatctac cggttgcccg ttctggaaca gatacacggt cggaatcgcg cgcagaccaa
    41881 actgggcggc aatcatctgc tccgcgtcgc agtccagctt cgccagaata aattgcccgt
    41941 tgtactgcgc cgcgaggctt tccaggatcg gggttaactg caaacagtgc tggctacgtt
    42001 cagaccaaaa atagaacagc accggagtgg tcatcgactg ttcaagaacc tgttgcaggt
    42061 tagattcgtt aatgttgaca atattttcta cggacatgga gtcgctctct gttgtcgata
    42121 ttttctttga catgggggct taagcgcgcg cttcaactca cccctgcaat attttgtcca
    42181 tcacgcgccc aggcagcagg cgctcgagca ccattactgc ccaggtcacc agcgttaccg
    42241 gatagcgcat cttcggcttc tcgctaataa aagcatggcg cactttgtcg accaccgctt
    42301 ccggtcccaa cgtaaagcgg gcggcgatgc cgggattttc gactggttta tcactttgcg
    42361 tctggttgac gttgtcggtg aagcgagtac gaatgggacc gggttcgatc aggctgactt
    42421 taattccgct gtggcgtagc tccatgcgca gcgcgtctga ccacgcctcc agcgcatatt
    42481 tactggccgc gtaagcgcca cgacccggcg tggagattaa tcccatcacc gatgatgtca
    42541 tcacaatacg cccttcaccg tgcggtaaca tcgcgggtaa caggcgcatg gtgagctggt
    42601 gtgcgccgaa aaagttggcg gaaaactgct gttccatctg cgcacggctg atggtggaaa
    42661 gggggccata catgccgaat ccggcattgt taaagatccc atacagacaa ttatcggtca
    42721 gggcgatcac ctcgtctgct gcgcgattaa cactttctgg tgaatccaga tcgatcaaca
    42781 cgccggtaaa tcccatgttg ttcatacgct caacatcatt cggttttcgg caacctgcca
    42841 gcacatgaaa accctggcgt tttaattcga gcgcgctttc caggccaatt ccactggaac
    42901 atccggtaat taagaccgat ttttgcataa ctttacctgt caggatctcc gttgctttat
    42961 gagtcatgat ttactaaagg ctgcaactgc ttcgccatcc agtcggcaat aaacggctgg
    43021 gcgtcgcggt tgggatgaat accgtcatcc tgcatccatt gtggcttgag gtagacctct
    43081 tccataaaaa agggcagcag cggaacatca aactctttgg cgagtttggg gtaaatggcg
    43141 ctaaaggctt cattataacg gcgaccatag tttgcaggca gacgtatttg cattaacaat
    43201 ggttcagcgt tggcggcttt gacatcctgc aaaatctggc gcagcgtttg ctcggtttgc
    43261 tgtggctgaa aaccacgcaa accatcattg cgcccagttc aaccagcacc caacgcggct
    43321 gatgctgttt gagcagagcc ggaaggcgcg ccagcccttg ttgcgaggtg tcgccgctga
    43381 tgctggcatt aactaccgac gttttactct gccacttatc attcaacaag gcaggccagg
    43441 ccgcgctggc agacattcga tacccggcgc tcaggctatc acccagaatc aataacgtgt
    43501 ccgctgcggc ggcacggaag gttaacagga ccaggaacag gaagggcaaa tgccagcgga
    43561 aaacattgtt gaagttcatc atcttaagaa gtccgtcggt cagggggagc atgaactctc
    43621 catcctcacc ggagttgagc tggttgtcaa acgtggcgag accatcgcac tggtgggcga
    43681 gtcgggatcg ggtaagtcaa ccttgctggc gatcctcgcc gggcttgatg acggcagcag
    43741 tggcgaagtg agtctggtgg gacaaccgct acataatatg gacgaagaag cgcgggcaaa
    43801 gttgcgcgcg aagcacgtcg gctttgtttt tcagtcattt atgttaattc ctacccttaa
    43861 cgcgctggaa aacgtcgagc ttccggctct gctgcgcggt gagagtagcg cggaaagtcg
    43921 taacggggcg aaagcgttgc tcgaacagtt agggctgggt aaacgtctgg atcatcttcc
    43981 ggcacagctt tccggcggtg aacagcaacg agtggcgctg gcacgagcct ttaatggtcg
    44041 acctgatgtg ctgtttgccg acgaacccac cggcaacctt gaccgccaga cgggcgataa
    44101 aattgccgac ctgctgtttt ccctcaaccg tgaacatggc accacgttga ttatggtgac
    44161 ccacgacctg caactggcgg cacgctgcga ccgctgctta cggctggtga acgggcagtt
    44221 gcaggaggaa gcatgattgc acgttggttc tggcgcgaat ggcgttcgcc gtcgctatta
    44281 attgtctggc tggcgctaag cctggcggtg gcctgcgtgc tggcgctggg caatatcagc
    44341 gatcgcatgg agaagggctt aagccagcaa agccgtgagt ttatggcggg cgatcgggcg
    44401 ttgcgcagtt cacgcgaagt gccgcaagcg tggctggagg aagcgcaaaa gcgcggcctg
    44461 aaagtcggca agcagctgac tttcgccaca atgacctttg caggcgacac accgcagctg
    44521 gcgaacgtca aagcggtgga tgatatctac ccgatgtatg gcggtctgca aactaatccc
    44581 cctggcctga aaccgcaggc gggcagcgta ttgctggccc cacgcctgat ggcactgctt
    44641 aacctgaaaa cgggcgacac cattgacgtg ggcgatgcca ccttgcggat tgccggagaa
    44701 gtgattcagg aaccggattc cggttttaac cccttccaga tggctccgcg tctgatgatg
    44761 aatctggcgg atgtcgataa aaccggagcc gtgcagccgg ggagtcgggt cacctggcgt
    44821 tataaattcg gcggcaacga gaaccagctc gacggctatg agaaatggtt gttacctcag
    44881 cttaaacccg aacaacgctg gtacggtctg gaacaggacg aaggcgcgct ggggcgatcg
    44941 atggaacgct cgcaacagtt cctgctgctt tcggcgcttc tgaccttgct gctggcagtg
    45001 gcagcggtgg cggtagcgat gaatcattac tgtcgcagtc gctacgatct ggtggcgatc
    45061 ctcaaaacgc tgggggcagg gcgagcgcaa ctgcgtaagc taatcgtcgg tcagtggttg
    45121 atggtgctga cgctttcagc cgttaccggt ggggccatag gcctgttgtt cgaaaacgtg
    45181 ttgatggtgc tgctcaagcc cgttctgcct gctgcactac cgccagccag cctctggccg
    45241 tggctgtggg cgcttggcac catgacggtc atctcgctgc tggtggggct acgaccatac
    45301 cgcttgttgc tggcaacgca gcctttacgc gtattacgta atgatgtggt cgcgaacgtc
    45361 tggccgctga agttttatct gccgattgtc agtgtggtgg ttgtgctgct gctcgccgga
    45421 ttaatgggtg gcagcatgct gctttgggcg gtgctggcgg gcgcggtagt actggctttg
    45481 ctgtgcggtg tgctgggctg gatgctgctg aatgtacttc gccgcatgac gctgaaatcg
    45541 ctgcctctgc gcctggcggt tagccgcctg ttacgtcagc cgtggtcaac gttaagccag
    45601 ctttcggcat tttcgctctc ctttatgctg ctggcactgc tgctggtgct gcgtggcgat
    45661 ctgctcgacc gctggcagca gcagctaccg ccagaaagcc cgaactactt tttaattaac
    45721 atcgccacag aacaggtagc accgctaaaa gcgttcctcg cggaacatca tatagtcccg
    45781 gaatcatttt atccggtagt gcgggcgcgg ctgacggcga ttaatgataa gccgacagaa
    45841 ggtaatgaag atgaggcgct taatcgcgaa ctcaatctta cctggcaaaa tacgcggccc
    45901 gatcataatc cgattgtcgc cggtaactgg ccgccaaaag ccgatgaagt gtcgatggaa
    45961 gaggggctgg cgaaacgctt aaacgttgcc ctcggtgata ccgtgacttt tatgggcgat
    46021 acccaggagt tccgcgctaa agtgaccagc ctgcgcaaag tggactggga aagtctgcgg
    46081 cctaatttct attttatttt ccctgaaggg gcattagacg ggcaaccgca gagctggctt
    46141 accagtttcc gctgggagaa tggcaacggc atgttgacgc aactcaaccg ccagttcccg
    46201 accattagcc tgttagacat tggcgcgatt ttaaaacagg tcggtcaggt gctggagcag
    46261 gtaagtcggg cgctggaggt gatggtggtg ctggtcaccg cctgcggtat gttgctgttg
    46321 ctggcacagg tgcaggtggg aatgcgccag cgtcatcagg agctggtggt atggcgcaca
    46381 ctcggtgcgg ggaaaaaact gctgcgcacg acattgtggt gtgatgttcg ccatgcttgg
    46441 gtttgtttcc ggcctggtgg ccgcaattgg tgcggaaacg gcactggcag tgttgcaggc
    46501 gaaagtgttt gatttcccgt gggagccaga ctggcgattg tggattgttc tgccgtgcag
    46561 cggtgcgctg ctgctgtcgc ttttcggcgg ctggctgggt gcgcgactgg ttaagggtaa
    46621 ggcgctgttc aggcagtttg tggggtgatg acaggtgtta gattgcctga tgcgctacgc
    46681 ttatcaggcc tacgaagagc gtgcaatata ttgattttgc aagattgtgt aggccggata
    46741 aggcgtttac gccgcatctg gcatgaacag tgtgcatttt ggcaataaat atgaaaccaa
    46801 ctgtcatgtc ggttttttta aaggccgaag gaggtaaacc ttcggcctta gctacgtttc
    46861 ctcagttatt caataaactg acggatcagt tccagcagcg cgattagcgc cttaagaacg
    46921 atgagaactt tatcgagaaa agttttcatc cgttgctccc ttaagggaga aagacaatca
    46981 cagcttaccc ttacgttgcc tataactatg attttcttca tgtgctagat gaggactgtc
    47041 tctctccaaa ttagagcacc agaatcagca ccacctgtat cctgaccagg acttaaaagt
    47101 acttttccgc actgcggtaa tcccttgagg cagtatcact actagcgtcg ggattgtatc
    47161 gccaacatag ctgtttgaat atgtaggcat cctgacttat aattcacttg ctagatgagg
    47221 actgtttaat ttctgcccgg aaccaccaag tcttgggcgg aaaaaataaa aaaaccgact
    47281 gtcatgtcgg tttttttata tctgtattct ggtgcaacca ttttgttctc acgacaatcg
    47341 ccgtttcaga ataccgccca tcactgcgcc ttaacccata ccgccacttc cgcccactca
    47401 ccgcgaaaga caactttttc cgcttttttc tcaagctgat agcgatacat tgggtcgtaa
    47461 tattctttaa gtaacggcac cagccaggcc agatgaccgt cggtgctgcc ggtggtgagt
    47521 tgcgttgtca gcgccgtatc cagctgggca gcgagttcgt tatagcgctg taaccccagc
    47581 cgacgcttaa tcgccgaaag tccgtgatgc aggtattcgc aatattcctg ccagccctgt
    47641 tcgtcgccat aagcgtgggt aaaatcatga tgcatacgca agaaatactc ttcattcagg
    47701 cgttcaagac ggatctcaaa cggatcttct accaccgcaa tcgccgcctg agtcattcgc
    47761 tcgcgcagac attccggcag gtgattcgaa ccgatcatcc ggctttcatc ttccagcacc
    47821 cacaggcgca aatcctgacg ggcgtcggtt tttagcattt cggcagccag caggttttca
    47881 aaactcgcct ggctaagttg tggttgtaac gtgcgaccaa acgccgaacc gcgatgacgc
    47941 gccagccctt ccagatcaac accgtttggc tgttgttgca ctaatagcgt tttaccgctg
    48001 ccggtacaac cgccaatcag cactatcggt ttttgtgcca gttcaatggt cgcctgaatc
    48061 gcggtctggc gcagtgcctt ataaccgcct tccaccagcg gataatcaat ccccgcttca
    48121 tgcagccagc gttgcacaat atgtgaacgt tgaccgccac gggcgcagca gagaatacct
    48181 tgcggatttt gcaggcacgc tgcccgccag gcgtacatac gctgctgacg aatttcaccc
    48241 gccaccagtt tatgccccag cgccagcgct gcgtctgagc cttgctgttt atagcaggtg
    48301 ccaacggagg cgcgttcatc gttatccatt aacggcagat tgatagcggc gggcattgcg
    48361 ccttgctcaa actcgatagg ggcgcgaaca tcaataatgg gcgtatcagc aatcagcagg
    48421 gcacgatagt cctgttccgt gtgtctctct tgcatagtta aaagtgaacc tcaaatcagc
    48481 ttgcgcgcta ttttacgcgc caacgcgcaa ggaaacttga tttttaactg cgtggggtgc
    48541 cgaaaatttc taaaaatccg ctgatttccg gcctgtgttg ggtaaacaag gtcacaatat
    48601 cttctaccgc tttgcagctg ccaaatttgc gccatgccag actcaatggc gaaggagggc
    48661 gcatcgttgg gattacccgg ctgaccagtt gttgattatc gatcattgac tggcaaagtg
    48721 attttggcaa aaaaccaatg ccaacacccg ccagatgggc ggcgattttc gtttccatat
    48781 cgggaacaat aatctctttt tgccccggca atcgccaggc gacgcgtttg gttaaggtgc
    48841 cggcgctgtc ttcaatattg accgccggaa agcgacgcaa ctgcgcttct gttagcggct
    48901 cttcaacgtt cgccagcgga tgatccgccg ccatgacaaa tcgccattgc accgatccta
    48961 agggatcaag actaaaggta tttgccagcg cctcagttcc cgtgaccccg atagccagcg
    49021 aaaaaccttc gtacaatagc gagtcccaga cgcccatata gatttgtcgg gagatgtgaa
    49081 actgggtaaa ggggtaacgc tcattcagcc acgccagcaa ctgggcgacg gcctgggggt
    49141 tatagagcag gttgttgatg acaatattca cctggcgttc cacgccatca ttcacctgtt
    49201 gcagctcgct gggcatactt tccagccagc tcagccagtc tctggcctgg gaaagtagat
    49261 gctcgccagc cgctgtcaac gttacgctac gagtcgtacg gaaaaacagc gctactccgg
    49321 tattctcttc cagaagttta atgcgataac tgatcgtcgc cgtggtttta cataatcgtt
    49381 ctgccgcttt tgaaaaactt cctgtttcag caaccgcaat gaaagtccgc aaggtttctg
    49441 gatcgaacat cttcaggtat ccccttttaa atccgcaagt tgcgtgattt tcttatcctc
    49501 tgatttatca gtatttttac atgataatcc tgttcaattt gtggactaaa tctagtttta
    49561 gaaaaacatt ccaacttttg tattaatgtt gttctcttaa ggttttagat tgcctgttat
    49621 tgaaaccaag ctgaccggtc ggcggtggtt gaacggaatt atgttacaag gacaaaaaga
    49681 tgaaacttca ggtattaccg ttaagtcagg aagcctttag tgcttatggc gacgtaatcg
    49741 aaacgcagca acgggatttt ttccatatta acaatggcct ggtggagcgt taccacgatt
    49801 tggcgctggt tgagattctt gagcaagacc gtacgcttat cagcattaac cgcgcgcaac
    49861 cggcgaatct gccgctgacc attcacgaac tcgaacgtca tccgctgggc actcaggcct
    49921 ttatcccaat gaaaggtgaa gtgtttgtgg tggtcgtggc gttaggcgac gacaaaccag
    49981 acctgtcaac gctgcgggcg tttatcacca acggtgaaca gggggtgaat taccatcgta
    50041 acgtctggca tcacccactt ttcgcctggc agcgcgtcac cgattttctg accatcgatc
    50101 gcggcggcag tgacaactgt gatgttgaaa gtattcctga acaggaactc tgttttgcgt
    50161 gacgcctgca accgacttgc ataagataaa ctaattgttc attgtttatg ctcacttgta
    50221 ggtcggagtt aacgtaggta tgacggaagt tagacggcgc ggcaggccag gacaggcgga
    50281 gcctgtggca cagaagggcg cacaggcgtt agagcgggga attgcgattc tgcaatatct
    50341 ggaaaaaagt gggggaagtt cgtcggttag cgatatttct ctcaatctgg atttgccgct
    50401 ctccacgacc tttcgcttgc tgaaggtttt acaagcagcg gattttgtct atcaggacag
    50461 tcaattaggc tggtggcata taggattagg tgtctttaac gtcggtgcgg cgtacatcca
    50521 taaccgcgat gtcctctccg tcgccgggcc gtttatgcgc cgcctgatgt tactttccgg
    50581 cgaaacggtc aatgtcgcga tccgtaacgg caatgaagcg gtattaattg gtcagttaga
    50641 gtgtaaatcg atggtcagga tgtgtgcgcc actgggcagt cgtctgccgc tgcatgcttc
    50701 cggtgcgggc aaagcgctgc tttatccgct ggcggaagag gagttgatga gcatcattct
    50761 gcaaaccggt ttgcagcagt ttacgccaac tacgcttgtg gatatgccca ccttgctgaa
    50821 ggacctggaa caagcgcgtg aactgggcta taccgtagat aaagaagagc atgttgtagg
    50881 tctgaattgc atagcttcag caatttacga tgatgtcggt agagttgttg ccgctatctc
    50941 catctccggg ccttcatcaa gactgacaga agatcgtttt gtcagtcagg gtgagctggt
    51001 cagagacacc gcccgcgata tcagcacggc gttgggactg aaagcacatc catagtgtct
    51061 gtcgcatccc gctctgcgga gcgggttttt ttgacaaaat ttgaaagttg gaaaattttt
    51121 ccaactaata gaggtaggaa taaaatggca aaaatgagag ccgttgacgc ggcaatgtat
    51181 gtgctggaga aagaaggtat caccaccgcc ttcggcgttc cgggagctgc aattaatccg
    51241 ttctactcag cgatgcgtaa gcacggcggt attcgtcaca ttctggcgcg tcatgtggaa
    51301 ggtgtttcgc acatggcgga aggttatacc cgcgcaacgg cagggaatat cggcgtatgt
    51361 ctggggactt ccggtcctgc gggcacggac atgatcaccg cgctctattc cgcttctgct
    51421 gattccattc ctattctgtg cattaccggc caggcaccgc gcgcccgtct gcataaagaa
    51481 gattttcagg ccgtagatat tgaagcaatt gctaaaccgg tcagcaaaat ggcggttaca
    51541 gttcgtgaag cggcgctggt gcctcgcgtg ctgcaacagg catttcacct gatgcgttct
    51601 ggtcgtccgg gtccggtact ggtggattta ccgttcgacg ttcaggttgc ggaaatcgag
    51661 tttgatcctg acatgtacga accgctgccg gtctacaaac ctgctgccag ccgtatgcag
    51721 atcgaaaaag ctgtagaaat gttaatccag gccgaacgtc cggtgattgt tgccgggggc
    51781 ggggtaatca atgctgacgc agctgcactg ttacaacagt ttgctgaact gaccagcgtt
    51841 ccggtgatcc caacgctgat gggctggggc tgtatcccgg acgatcatga actgatggcc
    51901 gggatggtcg gtctgcaaac cgcgcatcgt tacggtaacg caacgttgct ggcgtccgac
    51961 atggtgtttg gtatcggtaa ccgttttgct aaccgtcata ccggttcggt agagaaatac
    52021 accgaagggc gcaaaatcgt tcatattgat attgagccga cgcaaattgg tcgcgtgctg
    52081 tgtccggatc tcggcattgt ctctgatgct aaagcggcgc tgacactgct ggttgaagtg
    52141 gcgcaggaga tgcaaaaagc gggtcgtctg ccgtgtcgta aagaatgggt cgccgactgc
    52201 cagcagcgta aacgcacttt gctgcgcaaa acccacttcg acaacgtgcc ggtgaaaccg
    52261 cagcgcgtgt atgaagagat gaacaaagcc tttggtcgcg atgtttgtta tgtcaccact
    52321 attggtctgt cacaaatcgc tgcggcacaa atgctgcatg tctttaaaga ccgccactgg
    52381 atcaactgtg gtcaggctgg tccgttaggc tggacgattc cggcggcgct ggggggttgt
    52441 gccgctgatc cgaaacgcaa tgtggtggcg atttctggcg actttgactt ccagttcctg
    52501 attgaagagt tagccgttgg cgcgcagttc aacattccgt acatccatgt gctggtgaat
    52561 aacgcctatc tcggcctgat tcgtcagtca caacgcgctt ttgacatgga ctactgcgtg
    52621 caactcgctt tcgagaatat caactccagt gaagtgaatg gctacggtgt tgaccacgta
    52681 aaagtagcgg aaggtttagg ttgtaaagct attcgggtct tcaaaccgga agatattgcg
    52741 ccagcctttg aacaggcgaa agccttaatg gcgcaatatc gggtaccggt agtcgtggaa
    52801 gttattctcg agcgtgtgac caatatttcg atgggcagcg aactggataa cgtcatggaa
    52861 tttgaagata tcgccgataa cgcagcggat gcaccgactg aaacctgctt catgcactat
    52921 gaataaggga gataaatgat gttacgtttc tctgctaatt tatcgatgtt atttggtgaa
    52981 tatgattttc tcgcccgttt tgagaaagct gcgcagtgtg gttttcgcgg cgttgaattt
    53041 atgtttcctt atgactacga cattgaagaa ttaaaacagg tactggcgag taataaactc
    53101 gaacatacgc tgcacaattt accggcaggt gactgggcgg cgggcgagcg cggtattgcc
    53161 tgtattcctg gccgtgaaga agagtttcgg gatggcgtag cagcagcgat tcgttggtaa
    53221 tgactccaac ttactgatag tgttttatgt tcagataatg cccgatgacc ttgtcatgca
    53281 gctccaccga ttttgagaac gacagtgact tccgt
//
LOCUS       DAEBXN010000016        53187 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.016, whole genome shotgun sequence.
ACCESSION   DAEBXN010000016 DAEBXN010000000
VERSION     DAEBXN010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 53187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53187
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.016"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            351..437
                     /gene="leuL"
                     /locus_tag="KJL99_000935"
     CDS             351..437
                     /gene="leuL"
                     /locus_tag="KJL99_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HBD4435759.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            530..2101
                     /gene="leuA"
                     /locus_tag="KJL99_000936"
     CDS             530..2101
                     /gene="leuA"
                     /locus_tag="KJL99_000936"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HBD4435760.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVTNYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKTQHNENNKETV"
     gene            2101..3192
                     /gene="leuB"
                     /locus_tag="KJL99_000937"
     CDS             2101..3192
                     /gene="leuB"
                     /locus_tag="KJL99_000937"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HBD4435761.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDIQCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3195..4595
                     /gene="leuC"
                     /locus_tag="KJL99_000938"
     CDS             3195..4595
                     /gene="leuC"
                     /locus_tag="KJL99_000938"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HBD4435762.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAALVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4606..5211
                     /gene="leuD"
                     /locus_tag="KJL99_000939"
     CDS             4606..5211
                     /gene="leuD"
                     /locus_tag="KJL99_000939"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HBD4435763.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEAKQPAFMN"
     gene            complement(5260..6438)
                     /gene="setA"
                     /locus_tag="KJL99_000940"
     CDS             complement(5260..6438)
                     /gene="setA"
                     /locus_tag="KJL99_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HBD4435764.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLLSVARVELPSENALSMQGDWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSDLGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVVAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNTISTGVILAGVIQGAIAQSWGHFAVYWVIAIISVVALFLTAKVKDV
                     "
     gene            complement(6540..6671)
                     /gene="sgrT"
                     /locus_tag="KJL99_000941"
     CDS             complement(6540..6671)
                     /gene="sgrT"
                     /locus_tag="KJL99_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HBD4435765.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            6760..8415
                     /gene="sgrR"
                     /locus_tag="KJL99_000942"
     CDS             6760..8415
                     /gene="sgrR"
                     /locus_tag="KJL99_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HBD4435766.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIT
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPCEWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEKKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      8449..8548
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8579..9562
                     /gene="thiB"
                     /locus_tag="KJL99_000943"
     CDS             8579..9562
                     /gene="thiB"
                     /locus_tag="KJL99_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HBD4435767.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EANCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            9538..11148
                     /gene="thiP"
                     /locus_tag="KJL99_000944"
     CDS             9538..11148
                     /gene="thiP"
                     /locus_tag="KJL99_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HBD4435768.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            11132..11830
                     /gene="thiQ"
                     /locus_tag="KJL99_000945"
     CDS             11132..11830
                     /gene="thiQ"
                     /locus_tag="KJL99_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HBD4435769.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(11944..12708)
                     /locus_tag="KJL99_000946"
     CDS             complement(11944..12708)
                     /locus_tag="KJL99_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBD4435770.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(12794..13672)
                     /gene="araC"
                     /locus_tag="KJL99_000947"
     CDS             complement(12794..13672)
                     /gene="araC"
                     /locus_tag="KJL99_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HBD4435771.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGCHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPYFSDLFGQIINAGQGEGRYSELLAMNLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            14011..15711
                     /gene="araB"
                     /locus_tag="KJL99_000948"
     CDS             14011..15711
                     /gene="araB"
                     /locus_tag="KJL99_000948"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HBD4435772.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDTPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            15722..17224
                     /gene="araA"
                     /locus_tag="KJL99_000949"
     CDS             15722..17224
                     /gene="araA"
                     /locus_tag="KJL99_000949"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HBD4435773.1"
                     /translation="MAIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PYKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGYLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLPVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            17424..18119
                     /gene="araD"
                     /locus_tag="KJL99_000950"
     CDS             17424..18119
                     /gene="araD"
                     /locus_tag="KJL99_000950"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBD4435774.1"
                     /translation="MLKDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            18194..20545
                     /gene="polB"
                     /locus_tag="KJL99_000951"
     CDS             18194..20545
                     /gene="polB"
                     /locus_tag="KJL99_000951"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HBD4435775.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IHADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDKAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDIIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            20710..23616
                     /gene="rapA"
                     /locus_tag="KJL99_000952"
     CDS             20710..23616
                     /gene="rapA"
                     /locus_tag="KJL99_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HBD4435776.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKSCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            23628..24287
                     /gene="rluA"
                     /locus_tag="KJL99_000953"
     CDS             23628..24287
                     /gene="rluA"
                     /locus_tag="KJL99_000953"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HBD4435777.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(24404..25219)
                     /gene="djlA"
                     /locus_tag="KJL99_000954"
     CDS             complement(24404..25219)
                     /gene="djlA"
                     /locus_tag="KJL99_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HBD4435778.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            25474..27828
                     /gene="lptD"
                     /locus_tag="KJL99_000955"
     CDS             25474..27828
                     /gene="lptD"
                     /locus_tag="KJL99_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HBD4435779.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKFLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASRPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            27881..29167
                     /gene="surA"
                     /locus_tag="KJL99_000956"
     CDS             27881..29167
                     /gene="surA"
                     /locus_tag="KJL99_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HBD4435780.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            29167..30156
                     /gene="pdxA"
                     /locus_tag="KJL99_000957"
     CDS             29167..30156
                     /gene="pdxA"
                     /locus_tag="KJL99_000957"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HBD4435781.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDASIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            30153..30974
                     /gene="rsmA"
                     /locus_tag="KJL99_000958"
     CDS             30153..30974
                     /gene="rsmA"
                     /locus_tag="KJL99_000958"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HBD4435782.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDIRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            30977..31354
                     /gene="apaG"
                     /locus_tag="KJL99_000959"
     CDS             30977..31354
                     /gene="apaG"
                     /locus_tag="KJL99_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HBD4435783.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            31361..32203
                     /gene="apaH"
                     /locus_tag="KJL99_000960"
     CDS             31361..32203
                     /gene="apaH"
                     /locus_tag="KJL99_000960"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HBD4435784.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(32281..32760)
                     /gene="folA"
                     /locus_tag="KJL99_000961"
     CDS             complement(32281..32760)
                     /gene="folA"
                     /locus_tag="KJL99_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HBD4435785.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(32951..34813)
                     /gene="kefC"
                     /locus_tag="KJL99_000962"
     CDS             complement(32951..34813)
                     /gene="kefC"
                     /locus_tag="KJL99_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HBD4435786.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREA
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(34806..35336)
                     /gene="kefF"
                     /locus_tag="KJL99_000963"
     CDS             complement(34806..35336)
                     /gene="kefF"
                     /locus_tag="KJL99_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HBD4435787.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(35384..36778)
                     /locus_tag="KJL99_000964"
     CDS             complement(35384..36778)
                     /locus_tag="KJL99_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD4435788.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMC
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGSLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMGLFVQISL
                     FSESILLTISRVNI"
     gene            complement(36837..37124)
                     /gene="fixX"
                     /locus_tag="KJL99_000965"
     CDS             complement(36837..37124)
                     /gene="fixX"
                     /locus_tag="KJL99_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HBD4435789.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(37121..38407)
                     /locus_tag="KJL99_000966"
     CDS             complement(37121..38407)
                     /locus_tag="KJL99_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBD4435790.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVLLAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGNPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(38458..39399)
                     /locus_tag="KJL99_000967"
                     /pseudo
     CDS             complement(38458..39399)
                     /locus_tag="KJL99_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
     gene            complement(39414..40184)
                     /locus_tag="KJL99_000968"
     CDS             complement(39414..40184)
                     /locus_tag="KJL99_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HBD4435791.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            40540..42054
                     /gene="caiT"
                     /locus_tag="KJL99_000969"
     CDS             40540..42054
                     /gene="caiT"
                     /locus_tag="KJL99_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HBD4435792.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLAGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            42085..43227
                     /gene="caiA"
                     /locus_tag="KJL99_000970"
     CDS             42085..43227
                     /gene="caiA"
                     /locus_tag="KJL99_000970"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HBD4435793.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            43356..44573
                     /gene="caiB"
                     /locus_tag="KJL99_000971"
     CDS             43356..44573
                     /gene="caiB"
                     /locus_tag="KJL99_000971"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HBD4435794.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            44647..46200
                     /gene="caiC"
                     /locus_tag="KJL99_000972"
     CDS             44647..46200
                     /gene="caiC"
                     /locus_tag="KJL99_000972"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HBD4435795.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGNKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESE
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYM
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQAQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPS
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            46309..47094
                     /gene="caiD"
                     /locus_tag="KJL99_000973"
     CDS             46309..47094
                     /gene="caiD"
                     /locus_tag="KJL99_000973"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HBD4435796.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            47100..47690
                     /gene="caiE"
                     /locus_tag="KJL99_000974"
     CDS             47100..47690
                     /gene="caiE"
                     /locus_tag="KJL99_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HBD4435797.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(47776..48171)
                     /gene="caiF"
                     /locus_tag="KJL99_000975"
     CDS             complement(47776..48171)
                     /gene="caiF"
                     /locus_tag="KJL99_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HBD4435798.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMCR"
     gene            complement(48433..51654)
                     /gene="carB"
                     /locus_tag="KJL99_000976"
     CDS             complement(48433..51654)
                     /gene="carB"
                     /locus_tag="KJL99_000976"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBD4435799.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(51672..52820)
                     /gene="carA"
                     /locus_tag="KJL99_000977"
     CDS             complement(51672..52820)
                     /gene="carA"
                     /locus_tag="KJL99_000977"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBD4435800.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRIDKPAFSFQGHPEASPGPHDAAPLFDHFIKLIEQYRKTAK"
BASE COUNT        12792 a        14451 c        13940 g        12004 t
ORIGIN      
        1 aattcttaac attaattgtt caataatatt caccaaatca atatcaaaaa aaatcgcaaa
       61 acatataatt caatacaaat catcaggata ggttttgcaa cgcgtgcatt ttgtcccctt
      121 tttcctcgtt gattagatgc aaaaatttat gctgaaatat gtcaaccgat gaaaagcgtc
      181 ggtagttaag cagaaattaa tatcgcttac tttaaccacc gtagcacaat tagctaattt
      241 tacggatgca gaactcacgc tggcgggacg tttttattgc gtcagggttg acatccgttt
      301 ttgtatccag taactctaaa agcatatcgc attcatctgg agctgattta atgactcaca
      361 tcgttcgctt tatcggtcta ctactactaa acgcatcttc tttgcgcggt agacgagtga
      421 gcggcatcca gcattaagcc agcacgcagt caaacaaaaa acccgcgcca ttgcgcgggt
      481 ttttttatgc ccgaagcgag gcgctctaaa agagacaagg acccaaacca tgagccagca
      541 agtcattatt ttcgatacca cattgcgcga cggtgaacag gcgttacagg caagcttgag
      601 tgtgaaagaa aaactgcaaa ttgcgctggc ccttgagcgt atgggtgttg acgtgatgga
      661 agtcggtttc cccgtctctt cgccgggcga ttttgaatcg gtgcaaacca tcgcccgcca
      721 ggttaaaaac agccgcgtat gtgcgttagc tcgctgcgtg gaaaaagata tcgacgtggc
      781 ggccgaatcc ctgaaagtcg ccgaagcctt ccgtattcat acctttattg ctacctcccc
      841 aatgcacatc gccaccaagc tgcgcagcac cctagacgag gtgatcgaac gcgctatcta
      901 tatggtgaaa cgcgcccgta attacaccga tgatgttgaa ttttcttgcg aagatgccgg
      961 gcgtacaccc attgccgatc tggcgcgagt ggtcgaagcg gcgattaatg ccggtgccac
     1021 caccatcaac attccggaca ccgtgggcta caccatgccg tttgagttcg ccggaatcat
     1081 cagcggcctg tatgaacgcg tacctaacat cgacaaagcc attatttccg tacataccca
     1141 cgacgattta ggcctggcgg tcggcaactc actggcggcg gtacatgccg gtgcacgcca
     1201 ggtggaaggt gcaatgaacg ggatcggcga gcgtgccggt aactgttcgc tggaagaagt
     1261 catcatggcg attaaagttc gtaaggatat tctcaacgtc cacaccgcca ttaatcacca
     1321 agagatatgg cgcaccagcc agttagttag ccagatttgt aatatgccga tcccggcaaa
     1381 caaagccatt gttggcagcg gcgcattcgc tcactcctcc ggtatccacc aggatggcgt
     1441 gctgaaaaac cgcgaaaact acgaaatcat gacaccagaa tctattggtc tgaaccaaat
     1501 ccagctgaat ctgacctctc gttcggggcg tgcggcggtg aaacatcgca tggatgagat
     1561 ggggtataaa gaaagtgaat ataatttaga caatttgtac gatgctttcc tgaagctggc
     1621 ggacaaaaaa ggtcaggtgt ttgattacga tctggaggcg ctggccttca tcggtaagca
     1681 gcaagaagag ccggagcatt tccgtctgga ttacttcagc gtgcagtctg gctctaacga
     1741 tatcgccacc gccgccgtca aactggcctg tggcgaagaa gtcaaagcag aagccgccaa
     1801 cggtaacggt ccggtcgatg ccgtctatca ggcaattaac cgcatcactg actataacgt
     1861 cgaactggtg aaatacagcc tgaccgccaa aggtcacggt aaagatgcgc tgggtcaggt
     1921 ggatattgtc accaactaca acggtcgccg cttccacggc gtcggcctgg ccaccgatat
     1981 tgtcgagtcc tccgccaaag ccatggtgca cgtactgaac aatatctggc gtgccgcaga
     2041 agtcgaaaaa gagttgcaac gcaaaactca acacaacgaa aacaacaagg aaaccgtgtg
     2101 atgtcgaaga attaccatat tgccgtattg ccgggagacg gtattggtcc ggaagtgatg
     2161 acccaggcgc tgaaagtgct gaatgccgtg cgcaaccgct ttgcgatgcg catcaccacc
     2221 agccattacg atgtaggcgg cgcagccatt gataaccacg ggcaaccact gccgcctgcg
     2281 acggttgaag gttgtgagca agccgatgcc gtgctgtttg gctcggtagg cggcccgaag
     2341 tgggaacatt taccaccaga ccagcaacca gaacgcggcg cgctgctgcc gctgcgtaag
     2401 cacttcaaat tattcagcaa cctgcgcccg gcaaaactgt atcaggggct ggaagcattc
     2461 tgtccgctgc gtgcagacat tgccgctaac ggcttcgaca tccagtgcgt gcgcgaactg
     2521 accggcggca tctatttcgg tcagccaaaa ggccgcgaag gtagcggaca atatgaaaaa
     2581 gcgtttgata ccgaggtgta tcaccgtttt gagatcgagc gtattgcccg catcgcgttt
     2641 gaatctgctc gtaaacgtcg ccacaaagtc acctcaatcg acaaagctaa cgtgctgcaa
     2701 tcctctattt tatggcgtga aatcgtcaac gagatcgcca cggaataccc ggatgtcgaa
     2761 ctggcgcata tgtacatcga caacgccacc atgcagctga ttaaagatcc atcacagttt
     2821 gacgttctgc tgtgctccaa cctgtttggc gacattctgt ctgacgagtg cgcaatgatc
     2881 actggctcga tgggcatgtt gccttccgcc agcctgaacg agcaaggttt tggactgtat
     2941 gaaccggcgg gcggctcggc accagatatc gcaggcaaaa acatcgccaa cccgattgca
     3001 caaatcctgt cgctggcact gctgctgcgt tacagcctgg atgccgatga tgcggcttcc
     3061 gccattgaac gcgccattaa ccgtgcatta gaagaaggca ttcgcaccgg ggatttagcc
     3121 cgtggcgctg ccgccgttag taccgatgaa atgggcgata tcattgcccg ctatgtggca
     3181 gaaggggtgt aatcatggct aaaacgttat acgaaaaatt attcgacgct cacgtagtgt
     3241 acgaagccga aaacgaaacc ccgctgttat atatcgaccg ccacctggtg catgaagtga
     3301 cctcaccgca ggcgttcgat ggtctgcgcg cccacggtcg cccggtacgt cagccgggca
     3361 aaaccttcgc cactatggat cacaacgtct ctacccagac caaagacatt aatgcctgtg
     3421 gtgaaatggc gcgcatccag atgcaggagc tgattaaaaa ctgcaaagaa tttggcgtcg
     3481 agctgtatga cctgaatcac ccgtatcagg ggatcgtcca cgtaatgggg ccggaacagg
     3541 gcgtcacctt gccggggatg accattgtct gcggcgactc gcataccgcc acccacggcg
     3601 cgtttggcgc actggccttt ggtatcggca cttccgaagt tgaacacgta ctggcaacac
     3661 aaaccctgaa acagggccgt gcgaagacca tgaaaattga agtccagggc aaagccgcgc
     3721 cgggcattac cgcaaaagat atcgtgctgg caattatcgg taaaactggc agcgcaggcg
     3781 gcaccgggca tgtggtggag ttttgcggcg aagcaattcg tgatttaagc atggaaggtc
     3841 gtatgaccct gtgcaatatg gcgatcgaaa tgggcgcaaa agccgctctg gttgctcctg
     3901 acgaaaccac ctttaactat gtcaaaggcc gtctgcatgc gccgaaaggc aaagatttcg
     3961 acgacgccgt tgcctactgg aaaaccctgc aaaccgacga aggcgcaact ttcgataccg
     4021 ttgtcactct gcaagcagaa gaaatttcac cgcaggtcac ctggggcacc aatcccggcc
     4081 aggtgatttc tgtgaacgac aatattcccg atccggcttc gtttgccgat ccggttgaac
     4141 gtgcgtcggc agaaaaagcg ctggcctata tggggctgaa accgggtatt ccgctgaccg
     4201 aagtggctat cgacaaagtg tttatcggtt cctgtaccaa ctctcgcatt gaagatttac
     4261 gcgcggcagc ggagatcgcc aaagggcgaa aagtcgcgcc aggcgtgcag gcactggtgg
     4321 ttcccggctc tggcccggta aaagcccagg cggaagcgga aggtctggat aaaatattta
     4381 ttgaagccgg ttttgaatgg cgcttgcctg gctgctcaat gtgtctggcg atgaacaacg
     4441 accgtctgaa tccgggcgaa cgttgtgcat ccaccagcaa ccgtaacttt gaaggccgcc
     4501 aggggcgcgg cgggcgcacg catctggtca gcccggcaat ggctgccgct gctgctgtga
     4561 ccggacattt cgccgacatt cgcaacatta aataaggagc acaccatggc agagaaattt
     4621 atcaaacaca caggcctggt ggttccgctg gatgccgcca atgtcgatac cgatgcaatc
     4681 atcccgaaac agtttttgca gaaagtgacc cgtacgggtt ttggcgcgca tctgtttaac
     4741 gactggcgtt ttctcgatga aaaaggccaa cagccaaacc cggacttcgt gctgaacttc
     4801 ccgcagtatc agggcgcttc cattttgctg gcacgagaaa acttcggctg tggctcctcg
     4861 cgtgagcacg cgccctgggc attgaccgac tacggtttta aagtggtgat tgcgccgagt
     4921 tttgctgaca tcttctacgg caatagcttt aacaaccagc tgctgccggt gaaattaagc
     4981 gatgcagaag tggacgaact gtttgcgctg gtgaaagcca atccggggat ccatttcgac
     5041 gtggatctgg aagcgcaaga ggtgaaagcg ggagagaaaa cctatcgctt taccatcgat
     5101 gccttccgcc gccactgcat gatgaatggt ctggacagca tcggcctgac cctgcagcac
     5161 gacgacgcta ttgcgtctta tgaagcaaaa caacctgcgt ttatgaatta gaccccttgc
     5221 ccggtcaaat gaccgggctt tccgctatcg tccacgtcat caaacgtctt taacctttgc
     5281 ggttaaaaat aatgcgacaa cagaaataat cgcaattacc cagtagacag caaagtgccc
     5341 ccaactttgt gcaattgctc cctgaataac accagccaga attaccccgg tagagatagt
     5401 gttggtaaat aaggtggtag ctgcccccgc tcttccaggc attaaatcct gaaaccatag
     5461 catcccaata cccgcaacaa tgccgataaa tacagcgtta aaaagttgca gcgtcatcaa
     5521 cgccatacgg ctatgaaaga aaatcaatcc agtgtaaaac agcactcctg ccaccactgc
     5581 aatgaccatc attcgccgct taccataacg tttgacatag tagccagcca gaatcattgc
     5641 tggtatttcc agtccagctg ccgtccccat caggaaaccc gccagtttgt ctggcaatcc
     5701 taaatcgcta ctgatccaca atggcatatc aataatgtac atagtgttgc aggtccacat
     5761 taacgtcgag gcgacaaata acatccgtac gttactatcc tgccagtcac cttgcattga
     5821 taaagcattt tccgacggca gttctacccg cgccacagac agaagcataa atgcaatcaa
     5881 taccagactg agtgtgaata tcccggcggc aatcgaaaac atcactgtaa agccgtaatt
     5941 caacgccagc ataaaggcca gcggtggacc gattacccat gccagagaaa gctgcgcacg
     6001 catcaccgag ctaaacatca ccacttctcg cgccgagtta tccgcgtatt cccgcgccag
     6061 agcaaataac tgtggcattg ccgtattggc cagagatgcc agaagcacac cacaggtgat
     6121 aagcgtcaga taatgacgat taaatgcaaa cagtagcgca ttgccaatag ccatcaaaca
     6181 gcaaaatata atcagttttc gccgatcgcc ctgactgtca gaacgttttg ccaaccagag
     6241 gcttacgccg atcccagcaa tagcattcac cgtataaaag agtccgatcc agaaaggttg
     6301 cgcgccaacc tcacgactca gaaataagct caatgtagga gcctgtagcg ccccggccac
     6361 ccccatcata aaagcgacca gcataaatgc cgcgtaaaca ccgttcatac ggcgagccat
     6421 cgtcattatc cagatcatac gttccctttt tagcgcggcg agaataaaaa aaaccagcag
     6481 gtataatctg ctggcgggtg attttacacc aatactcagt cacacatgat gcaggcaagt
     6541 caactttcag aattgcggtc atcccactgc atcagtcctt caagcatggt taatcgttgt
     6601 gggacgctta accaacgcaa ccagcacaac ttcgctgtcg cggtaaaata gtgctgataa
     6661 aactgacgca tggggcacct ccttgcttca tcgttaggaa ttattgactt aatataggga
     6721 aaataaaatt gctgtctttt gcgcaggagt tcccctttta tgccatctgc tcgtctgcaa
     6781 caacagttca tccgcctgtg gcaatgctgc gagggtaaat cgcaggacac aacgctcaac
     6841 gaactggcag cgttattgag ctgctcgcgt cgtcatatgc gtaccctgct caacaccatg
     6901 caggatcgcg gctggctgac gtgggaagcg gaagtcgggc gcggtaaacg ctcgcgtctg
     6961 acattcctct ataccgggct ggcgcttcag caacagcggg cggaagacct gctggagcag
     7021 gatcgtatcg accaactagt gcagttggtt ggcgacaaag cgactgtgcg gcaaatgctg
     7081 gtttctcatc tgggccgcag cttccgccag gggcggcaca tcctgcgcgt gctctactat
     7141 cgtccgttgc gtaatctgct acctggcagc gcattgcgcc gttccgaaac ccatattgcc
     7201 cggcaaatct tcagttcgct aacgcgcata aatgaggaaa atggggaact ggaagcagac
     7261 atcgcccacc actggcagca aatatcaccg cttcactggc gtttcttttt gcgtccagga
     7321 gtccattttc atcatggtcg tgaactggaa atggacgacg tgatcgcctc tttaaaacga
     7381 atcaatacgc tgccgctcta ttcgcatatt acggacattg tgtcgccgac gccctggacg
     7441 ctggatatcc atctcacgca gccggaccgc tggttgccgt tactgctggg gcaagttccg
     7501 gcgatgatcc tgccgtgcga atgggaaacc ctcagtaact ttgccagcca tcctattggc
     7561 accggtccgt atgcggtgat tcgcaacagc accaatcaac tgaaaattca ggcattcgat
     7621 gacttcttcg gttaccgggc attaatcgac gaagttaacg tctgggttct gccggaaatt
     7681 gccgacgagc cagccggtgg tctgatgcta aaaggtccac agggcgagaa aaaagagatt
     7741 gaaagccgcc tggaggaagg ttgctactat ttactgttcg acagccgcac ccatcgcggg
     7801 gcgaatcagc aagtcaggga ctgggtaagc tatgtgcttt ctccaactaa tctggtctat
     7861 ttcgctgagg aacagtacca gcaactgtgg ttcccggctt atggactgct cccccgttgg
     7921 caccatgccc gcaccataaa gagcgaaaaa ccggctggcc tggaaagcct caccctgacc
     7981 ttttatcagg atcacagtga gcatcgggtg attgccggga tcatgcagca gattctggca
     8041 agtcaccagg tcacgctgga aatcaaagag atcagctacg atcagtggca tgaaggagag
     8101 atcgagagcg atatctggct taatagcgcc aactttacgc tgccgctgga tttttcgctg
     8161 ttcgcgcact tgtgcgaggt gccgctgctc caacactgta ttccgatcga ctggcaagcc
     8221 gacgccgccc gctggcgcaa tggcgaaatg aacctggcga actggtgcca gcaactggtc
     8281 gccagtaaag caatggtgcc gcttatccac cactggctga tcattcaggg acaacgcagt
     8341 atgcgcggcc tgcgtatgaa taccctcggc tggttcgatt ttaaatcagc gtggtttgcg
     8401 ccaccggatc catgattgct ggtagataac aaaatcacta cactaacgcc gttctcaacg
     8461 gggtgccacg caaacgcgtg cgctgagaaa atacccgtcg aacctgatcc ggataacgcc
     8521 ggcgaaggga tttgaggctc cttctcaagt cctttgccac tcttttttga ggtgcaaagt
     8581 gttaaaaaaa tgtcttcccc tgctgttgct gtgcacagcg cccgttttcg ctaaacccgt
     8641 tctgactgtt tatacctacg attccttcgc cgccgactgg gggcctggtc caaagatcaa
     8701 aaaagctttt gaagccaact gtaactgcga actgaaactg gtggcgctgg aagatggcgt
     8761 ttcgcttctc aaccgtctac ggatggaagg caaaaatagt aaagccgatg tggtgctggg
     8821 gctggataac aatctgttag acgccgccag taaaaccgga ctatttgcca aaagcggtgt
     8881 ggcagcggat gccgttaacg ttcccggcgg ctggaataat gacactttcg tcccgtttga
     8941 ttacggctat ttcgccttcg tctatgacaa gaacaaactg aaaaacccgc cgcaaagcct
     9001 gaaagaattg gttgagagcg atcagaactg gcgggtgatt tatcaggatc cgcgtaccag
     9061 cacgccgggg ctgggtctgc tgttgtggat gcaaaaagtc tatggcgatg acgccccaca
     9121 agcctggcaa aaactggcga agaaaaccgt cacggtcacc aaaggctgga gcgaagccta
     9181 cggcctgttt ttaaaaggtg aaagcgatct ggtactgagt tacaccacct ctccggctta
     9241 tcacattctc gaagagaaaa aagataacta cgccgccgcg aactttagcg aaggtcacta
     9301 tctgcaggtg gaagtcgccg cccgcaccgc tgccagcaag cagccggagc tggcgcaaaa
     9361 attcctccag tttatggttt ctccggcttt tcagaatgcg atcccaaccg gtaactggat
     9421 gtatccggtg gcaaatgtca cgctgcctgc cgggtttgaa caattgacca aaccggccac
     9481 cacgctggag ttcacaccag ctgaagtggc ggcacaacgt caggcatgga ttagcgaatg
     9541 gcaacgcgcc gtcagccgtt aattcccggc tggttaattc caggtgtaag cgccgccacg
     9601 ctggtggtag cggttgcgct cgcggcgttt ctcgccctgt ggtggaacgc gccgcagggt
     9661 aactgggtgg cagtctggca ggacagctac ctgtggcatg tggtgcgctt ctccttctgg
     9721 caggcgtttc tctcggcgct actctctgtc gtacccgcga tattcctcgc ccgcgccctc
     9781 tatcgcaggc gctttccggg tcggctggcg ctgttgcgtc tgtgcgcaat gaccttgatc
     9841 ctcccggtgc tagttgctgt tttcggcatt cttagcgtct atggtcgtca gggctggctg
     9901 gcatcgctct gccaatcgct cggtctggag tggacctttt cgccctacgg cctgcaaggc
     9961 attttgctgg cgcacgtatt ttttaatctg ccgatggcga gccgcttatt actccaggca
    10021 ctggaaaaca ttcccggcga acaacgtcag cttgccgccc agcttgggat gcgcggctgg
    10081 cattttttcc gcttcgtcga atggccgtgg ctacggcgac aaatcccgcc ggttgccgcg
    10141 cttatcttta tgctctgttt cgccagcttc gccaccgtgc tatcgctggg cggcggtccg
    10201 caggcaacca ctatcgagct ggctatttat caggcgctga gttacgacta cgatcctgcc
    10261 cgcgcggcga tgctggcgct gctccagatg gtgtgttgcc ttgggctggt gctgttgagt
    10321 cagcgactaa gtaaggccat tgcgcccggc accacgctgc tgcaaggctg gcgcgatccg
    10381 gacgatcgtc tgcatagccg catttgcgac acggtgttaa tagtgctggc gctgctgctg
    10441 ttgctgccac cgttgctggc ggtgatcgtc gatggggtaa atcgccagtt gccggaagtg
    10501 ctggcacaac cggtgctgtg gcaggcgctg tggacctcgt tgcgtattgc gctggcggca
    10561 ggtgtattgt gcgtagtgct gaccatgatg ctgctatgga gcagtcgcga actgcgggcg
    10621 cggcagaaaa tgctggcggg tcaggcgctg gagatgagcg gcatgttgat cctcgccatg
    10681 ccggggattg tgctggctac cggcttcttt ttactgctca acaacactat cggcctgcca
    10741 caatctgctg acggcattgt gattttcacc aatgcgttaa tggcgatccc ttatgcgctg
    10801 aaagtactgg aaaacccgat gcgcgatatc accgcccgct acagtatgtt gtgtcagtcg
    10861 ctgggcattg aaggctggtc gcgcttaaaa gtggtcgagc tgcgcgccct gaaacgtcca
    10921 ctggcgcagg cgctggcttt tgcctgcgtg ctgtcgattg gtgattttgg cgtagtggcg
    10981 ttgttcggta acgatgattt ccgtaccctg ccgttttatc tctaccagca aattggctcc
    11041 tatcgcagcc aggacggcgc ggtcaccgcg ttaattctgc tactactctg ttttctgctg
    11101 tttaccgtga ttgaaaaact accggggcga aatgttaaaa ctgactgata tcacctggct
    11161 ttatcaccat ttgccgatgc gttttagctt aacggtagaa cgcggcgagc aggtggcaat
    11221 cctcgggcca agcggcgcgg gtaaaagtac cctgctgaat ttgattgccg gttttctgac
    11281 gccagccagc ggttcgctga ctatcgatgg tgttgatcac accacgacgc cgccgtcacg
    11341 ccgtccggtg tcgatgctgt ttcaggagaa caacctgttc agccatctga cggtcgcgca
    11401 gaacatcggg ctggggctaa atccggggtt gaaactgaac gcggcacagc aggagaaaat
    11461 gcacgcgatt gcccgtcaga tggggattga taatttaatg gcgcggttac cgggcgagct
    11521 ttccggcggt cagcgacagc gcgtggcgtt agcgcgttgt ctggtgcgcg aacagccgat
    11581 tttattgctc gatgaaccgt tctctgcgct cgatccggcg ttacgtcagg agatgctgac
    11641 gctggtgagc acgagctgcc agcagcaaaa aatgacgcta ttgatggtgt cgcacagtgt
    11701 ggaagatgcg gcgcggatcg ccacacgctc ggtggtggtc gccgacgggc gcatcgcctg
    11761 gcagggtaag actgaagaat tgttgagcgg taaggcgagt gcttcggcaa tattggggat
    11821 tacgggttag tatgcactgt cagatgcggc gtgaacacct tatccgacct acggacagtg
    11881 cgttttgtag gcctgataag acgcggtcag cgtcgcatga ggcaacccgc acaagacaat
    11941 cccctaaacc ccaaccactt tacgcaaaat atcgatatac accggcatca acgggtggcg
    12001 aattaacacc accagcgcca ccacgccgat ggcagaaatc aacggcgtca gccacaacaa
    12061 acggccgcgc gacaaataat gacttaaacg atcggttgct ttaccgctgc gccataaccg
    12121 ccagcacagc cagccaccaa cccacaaaaa taccgccgtc gccagcagca accatttaaa
    12181 ctcgccgctc tgcattccgg ctggaatatc gatcgccgcg cccgccagaa tcccaggcag
    12241 gaagtaaaac ggtggccaca gcaggcagcc gataatattc ggcgtaatga attttgccac
    12301 cggcagatcc agcattcccg ccaccattgg caccagcgga cgcgtcgggc caacaaagcg
    12361 accgaccaga atggtgaaca tgctgtgttg atgcaacgca tgttcagttt tatcgagtaa
    12421 tgctttgttt ttcttcagaa atgaccagcg atgcaacggc tttttaaaac gccagcccag
    12481 ccagaaggag atccagtcgc ccagcagaca accgacaatc cctgccagcc aagcgtgcca
    12541 gaaacttaac tcgccgctgc caatcagcgc tcccagcccc gccatcagca ccgtaccggg
    12601 tagaatcaaa ccgaccagcg ccagcgactc cagaaaggcc accaacacca ccgccatcaa
    12661 tgaatacacg gtagattggg taataaagtg ttccagcaat gcttgcataa tgtgcctgtc
    12721 aaatggacga agcagggatt ctgcaaaccc tatgctactc cgtcaagccg tcaattgtct
    12781 gattcgttac caattatgac aacttgacgg ctacatcatt cactttttct tcacaaccgg
    12841 cacggaactc gctcgggctg gccccggtgc attttttaaa tacccgcgag aaatagagtt
    12901 gatcgtcaaa accaacattg cgaccgacgg tggcgatagg catccgggtg gtgctcaaaa
    12961 gcagcttcgc ctggctgata cgttggtcct cgcgccagct taagacgcta atccctaact
    13021 gctggcggaa aagatgtgac agacgcgacg gcgacaagca aacatgctgt gcgacgctgg
    13081 cgatatcaaa attgctgtct gccaggtgat cgctgatgta ctgacaagcc tcgcgtacac
    13141 gattatccat cggtggatgg agcgactcgt taatcgcttc catgcgccgc agtaacaatt
    13201 gctcaagcag attcatcgcc agcagctccg aatagcgccc ttccccttgc ccggcgttaa
    13261 tgatttgccc aaacaggtcg ctgaaatacg gctggtgcgc ttcatccggg cgaaagaaac
    13321 ccgtattggc aaatattgac ggccagttaa gccattcatg ccagtaggcg cgcggacgaa
    13381 agtaaaccca ctggtgatac cattcgcgag cctccggatg acaaccgtag tgatgaatct
    13441 ctcctggcgg gaacagcaaa atatcacccg gtcggcagac aaattctcgt ccctgatttt
    13501 tcaccacccc ctgaccgcga atggtgagat tgagaatata acctttcatt cccagcggtc
    13561 ggtcgataaa aaaatcgaga taaccgttgg cctcaatcgg cgttaaaccc gccaccagat
    13621 gggcattaaa cgagtatccc ggcagcaggg gatcattttg cgcttcagcc atacttttca
    13681 tactcccgcc attcagagaa gaaaccaatt gtccatattg catcagacat tgccgtcact
    13741 gcgtctttta ctggctcttc tcgctaaccc aaccggtaac cccgcttatt aaaagcattc
    13801 tgtaacaaag caggaccaaa gccatgacaa aaacgcgtaa caaaagtgtc tataatcacg
    13861 gcagaaaagt ccacattgat tatttgcacg gcgtcacact ttgctatgcc atagcatttt
    13921 tatccataag attagcggat cctacctgac gctttttatc gcaactctct actgtttctc
    13981 catacccgtt tttttggatg gagtgaaacg atggcgattg caattggcct cgattttggc
    14041 agtgattctg tgcgagcttt ggcggtggac tgcgccagcg gtgaagagat cgccaccagc
    14101 gtagagtggt atccccgttg gcaaaaaggg caattttgtg atgccccgaa taaccagttc
    14161 cgtcatcatc cgcgtgacta cattgagtca atggaagcgg cactgaaaac cgtgcttgca
    14221 gagcttagcg tcgaacagcg cgcagctgtg gtcgggattg gcgttgacag taccggctcg
    14281 acgcccgcac cgattgatgc cgacggtaac gtgctggcgc tgcgcccgga gtttgccgaa
    14341 aacccgaacg cgatgttcgt attgtggaaa gaccacactg cggttgaaga agcggaagag
    14401 attacccgtt tgtgccacgc gccgggcaac gttgactact cccgctatat tggcggtatt
    14461 tattccagcg aatggttctg ggcaaaaatc ctgcatgtga ctcgccagga cagcgccgtg
    14521 gcgcaatctg ccgcatcgtg gattgagctg tgcgactggg tgccagctct gctttccggt
    14581 accacccgcc cgcaggatat tcgtcgcgga cgttgcagcg ccgggcataa atctctgtgg
    14641 cacgaaagct ggggcggctt gccgccagcc agtttctttg atgagctgga cccgatcctc
    14701 aatcgccatt tgccttcccc gctgttcact gacacctgga ctgccgatat tccggtgggc
    14761 accttatgcc cggaatgggc gcagcgtctc ggcctgcctg aaagcgtggt gatttccggc
    14821 ggcgcgtttg actgccatat gggcgcagtt ggcgcaggcg cacagcctaa cgcactggta
    14881 aaagttatcg gtacttccac ctgcgacatt ctgattgccg acaaacagag cgttggcgag
    14941 cgggcagtta aaggtatttg cggtcaggtt gatggcagcg tggtgcctgg atttatcggt
    15001 ctggaagcag gccaatcggc gtttggtgat atctacgcct ggtttggtcg cgtactcggc
    15061 tggccgctgg aacagcttgc cgcccagcat ccggaactga aagcgcaaat caacgccagc
    15121 cagaaacaac tgcttccggc gctgaccgaa gcatgggcca aaaatccgtc tctggatcac
    15181 ctgccggtgg tgctcgactg gtttaacggt cgtcgcacgc caaacgctaa ccaacgcctg
    15241 aaaggggtga ttaccgatct taacctcgct accgacactc cgctgctgtt cggcggtttg
    15301 attgctgcca ccgcctttgg cgcacgcgca atcatggagt gctttaccga tcaggggatc
    15361 gccgtcaata acgtgatggc gctgggcggc atcgcgcgga aaaaccaggt cattatgcag
    15421 gcctgctgcg acgtgctgaa tcgcccgctg caaattgttg cctctgacca gtgctgtgcg
    15481 ctcggtgcgg cgatttttgc tgccgtcgcc gcgaaagtgc acgcagacat cccatcagcc
    15541 cagcaaaaaa tggccagtgc ggtagagaaa accctgcaac cgcgcagcga acaggcacaa
    15601 cgctttgaac agctttatcg ccgctatcag caatgggcga tgagcgccga acaacactat
    15661 cttccaactt ccgccccggc acaggctgcc caggccgttg cgactctata aggacacgat
    15721 aatggcgatt tttgataatt atgaagtgtg gtttgtcatt ggcagccagc atctgtatgg
    15781 cccggaaacc ctgcgtcagg tcacccaaca tgccgagcac gttgttaatg cgctgaatac
    15841 ggaagcgaaa ctgccctaca aactggtgtt gaaaccgctg ggcaccacgc cggatgaaat
    15901 caccgctatt tgccgcgacg cgaattacga cgatcgttgc gctggtctgg tggtgtggct
    15961 gcacaccttc tccccggcca aaatgtggat caacggcctg accatgctca acaaaccgtt
    16021 gctgcaattc cacacccagt tcaacgcggc gctgccgtgg gacagtatcg atatggactt
    16081 tatgaacctg aaccagactg cacatggcgg tcgcgagttc ggcttcattg gcgcgcgtat
    16141 gcgtcagcaa catgccgtcg ttaccggtca ctggcaggat aaacaagccc atgagcgtat
    16201 cggctcctgg atgcgtcagg cggtctctaa acaggatacc cgtcatctga aagtctgccg
    16261 atttggcgat aacatgcgtg aagtggcggt caccgatggc gataaagttg ccgcacagat
    16321 caagttcggt ttctccgtca atacctgggc ggttggctat ctggtgcagg tggtgaactc
    16381 catcagcgac ggcgatgtta acgcgctggt cgatgagtac gaaagctgct acaccatgac
    16441 gcctgccaca caaatccacg gcgaaaaacg acagaacgtg ctggaagcgg cgcgtattga
    16501 gctggggatg aagcgtttcc tggaacaagg tggcttccac gcgttcacca ccacctttga
    16561 agatttgcac ggtctgaaac agcttccagg tctgcccgta cagcgtctga tgcagcaggg
    16621 ttacggcttt gcgggcgaag gcgactggaa aaccgccgcc ctgcttcgca tcatgaaggt
    16681 gatgtcaacc ggtctgcagg gcggcacctc ctttatggag gactacacct atcacttcga
    16741 gaaaggtaat gacctggtgc tcggctccca tatgctggaa gtctgcccgt cgattgccgt
    16801 agaagagaaa ccgatcctcg acgttcagca tctcggtatt ggtggtaagg acgatcctgc
    16861 ccgactgatc ttcaataccc aaaccggtcc agcgattgtc gccagcctga ttgatctcgg
    16921 cgatcgttac cgtctgctgg ttaactgtat cgacacggtg aaaacaccgc actccctgcc
    16981 gaaactgccg gtggcgaatg cactgtggaa agcgcaaccg gatctgccaa ctgcttccga
    17041 agcgtggatc ctcgctggtg gcgcgcacca taccgtcttc agccatgcgc tgaacctcaa
    17101 cgatatgcgc cagttcgccg agatgcacga cattgaaatc acggtgattg ataacgacac
    17161 ccgcctgcca gcgtttaaag acgcactgcg ctggaacgaa gtgtattacg gatttcgtcg
    17221 ctaagtagcc acatcaggta tgtaacgcct gatgcgacgc tgacgcgtct tatctggcct
    17281 acacgctgcg attttgtagg ccggataagc aaagcgcatc cggcattcaa cgcctgatgc
    17341 gacgctggcg cgtcttatca ggcctacgcg ctgcgatttt gtagaccgga taagcgaagc
    17401 gcatccggca cgaaggagtc aacatgttaa aagatctcaa acgccaggta ttagaagcca
    17461 acctggcgct gccaaaacac aacctggtca cgctcacatg gggcaacgtc agcgccgttg
    17521 atcgcgagcg cggcgtcttt gtgatcaaac cttccggcgt cgattacagc gtcatgaccg
    17581 ctgacgatat ggtcgtggtt agcatcgaaa ccggtgaagt ggttgaaggt acgaaaaagc
    17641 cctcctccga cacgccaact caccggctgc tctatcaggc attcccctcc attggcggca
    17701 ttgtgcatac gcactcgcgc cacgccacca tctgggcgca ggcgggtcag tcgattccag
    17761 caaccggcac cacccacgcc gactatttct acggcaccat tccctgcacc cgcaaaatga
    17821 ccgacgcaga aatcaacggc gaatatgagt gggaaaccgg taacgtcatc gtagaaacct
    17881 ttgaaaaaca gggtatcgat gcagcgcaaa tgcccggcgt tctggtccat tcccacggcc
    17941 cgtttgcatg gggcaaaaat gccgaagatg cggtgcataa cgccatcgtg ctggaagagg
    18001 tcgcttatat ggggatattc tgccgtcagt tagcgccgca gttaccggat atgcagcaaa
    18061 cgctgctgga taaacactat ctgcgtaagc atggcgcgaa ggcatattac gggcagtaat
    18121 gactgtataa aaccacagcc aatcaaacga aaccaggcta tactcaagcc tggttttttg
    18181 atggattttc agcgtggcgc aggcaggttt tatcttaacc cgacactggc gggacacccc
    18241 gcaagggaca gaagtctcct tctggctggc gacggacaac gggccgttgc aggttacgct
    18301 tgcaccgcaa gagtccgtgg cgtttattca cgccgatcag gttccccgcg ctcagcatat
    18361 tttgcagggt gaacaaggct ttcgtctgac accgctggcg ttaaaggatt ttcaccgcca
    18421 gccggtgtat ggcctttact gtcgcgccca tcgccaattg atgaattacg aaaagcgtct
    18481 gcgtgaaggt ggcgttaccg tctacgaggc cgatgtgcgc ccgccagaac gctatctgat
    18541 ggagcggttt atcacctcac cggtgtgggt cgagggtgat atgcacaatg gcactatcgt
    18601 taatgcccgt ctgaaaccgc atcccgacta tcgtccgccg ctcaagtggg tttctataga
    18661 tattgaaacc acccgccacg gtgagctgta ctgcatcggc ctggaaggct gcgggcagcg
    18721 catcgtttat atgctggggc cggagaatgg cgacgcctcc tcgcttgatt tcgaactgga
    18781 atacgtcgcc agccgcccgc tgctgctgga aaaactcaac gcctggtttg caaaccacga
    18841 tcccgatgtg atcatcggct ggaacgtggt gcagttcgat ctgcgaatgc tgcaaaaaca
    18901 tgccgagcgt taccgtcttc cgctgcgtct tgggcgcgat aatagcgagc tggagtggcg
    18961 cgagcacggc tttaaaaacg gcgtcttttt tgcccaggct aaaggtcggc taattatcga
    19021 cggtatcgag gcgctgaaat ccgcgttctg gaatttctct tcattctcgc tggaaactgt
    19081 cgctcaggag ctattaggcg aaggaaaatc tatcgataac ccgtgggatc gaatggacga
    19141 aattgaccgc cgtttcgccg aagataaacc tgcgctggca acttataacc tgaaagattg
    19201 cgagctggtg acgcagatct tccacaaaac tgaaatcatg ccatttttac tcgaacgagc
    19261 aacggtgaac ggcctgccgg tggatcggca cggcggttcg gtggcagcgt ttggtcatct
    19321 ctattttccg cgaatgcatc gcgctggtta tgtcgcgcct aatctcggcg aagtgccgcc
    19381 gcacgccagc cctggcggct acgtgatgga ttcacggcca gggctttatg attcagtgct
    19441 ggtgctggac tataaaagcc tgtacccgtc gatcatccgc acctttctga ttgatcccgt
    19501 cgggctggtt gaaggcatgg cgcaacctga tccagagcac agtaccgaag gttttctcga
    19561 tgcctggttc tcacgggaga aacattgcct gccggagatt gtgactaaca tctggcacgg
    19621 gcgcgataaa gccaaacgtc agggtaataa gccgctttcg caggcgctga agatcattat
    19681 gaatgccttt tatggcgtgc tcggcaccac cgcctgccgc ttcttcgatc cgcgcctggc
    19741 atcgtcgatc accatgcgtg gtcatcagat catgcggcag accaaagcat tgattgaagc
    19801 gcagggctac gacattatct acggagatac cgactcgact tttgtctggc taaaaggtgc
    19861 acattcggaa gaagaggcgg cgaaaatcgg tcgtgcactg gtgcagcacg ttaacgcctg
    19921 gtgggcggaa acgctgcaaa aacaacggct gaccagcgca ttagaactgg agtatgaaac
    19981 tcatttctgc cgttttctta tgccaaccat tcgcggagct gataccggca gtaaaaagcg
    20041 ctacgccggg ctgattcagg agggcgacaa gcagcggatg gtgtttaaag ggctggaaac
    20101 cgtgcgcacc gactggacgc cgctggccca gcagtttcag caggagctgt acctgcgtat
    20161 cttccgcaac gagccatatc aggagtacgt ccgccaaacc atcgacaaac tgatggcggg
    20221 tgaactggat gcgcggctgg tttaccgtaa acgccttcgc cgtccgctga gcgaatatca
    20281 gcgtaatgtt ccgcctcatg tacgcgccgc tcgccttgcc gatgaagaaa atcaaaagcg
    20341 cggtcgcccc ttgcaatatc agaatcgcgg caccattaag tacgtatgga ccaccaacgg
    20401 cccggagccg ctggactacc aacgttcacc actggattac gaacactatc tgacccgcca
    20461 gctacaaccc gtggcggagg gaatactccc ttttattgag gataattttg ctacacttat
    20521 gaccgggcaa cttgggctat tttgagcaaa aaaaagagtt cgccagatac cattttgatg
    20581 cgtgacgaat gctttgccat ccagtaccat agcgcccttt ccattcctgg acctgaataa
    20641 caccactacc tcataagcac ggtagcgggt ggttattgcc tgcaattaaa gatatagagc
    20701 cgaacacata tgccttttac acttggtcaa cgctggatca gcgatacaga aagcgaattg
    20761 ggacttggaa ccgttgtcgc ggtggatgcg cgaactgtca ctttactttt cccatctact
    20821 ggtgaaaacc gtctgtacgc acgcagtgat tcccccgtga cccgcgtgat gttcaaccct
    20881 ggtgatacca ttaccagcca tgacggctgg cagatgcaag tcgaagaagt aaaagaagaa
    20941 aatggcttgc tgacctatat cggtactcgc ctggatactg aagagtccgg cgtagccctg
    21001 cgtgaagttt tccttgatag caaactggtg ttcagcaaac cgcaggaccg tctgtttgcc
    21061 gggcagattg accgtatgga ccgctttgcg ctgcgttatc gcgcgcgtaa gtattccagc
    21121 gaacagttcc gtatgccgta cagcggcctg cgcggtcagc gtaccagcct gatcccgcat
    21181 cagctcaaca tcgctcatga tgttggtcgc cgccacgcgc cgcgcgtcct gctggctgac
    21241 gaagtgggtt tagggaaaac cattgaagcc gggatgatcc tgcatcagca actgctctct
    21301 ggcgctgctg aacgtgtgct gattatcgtc ccggaaacct tacagcatca gtggctggta
    21361 gaaatgctgc gccgtttcaa cctgcgcttt gctctgtttg atgatgagcg ttatgccgaa
    21421 gctcagcacg atgcttacaa cccgtttgac accgaacagc tggtgatttg ctcgctggat
    21481 tttgcccgtc gtagcaaaca gcgcttggaa catctctgtg aagccgaatg ggacctgctg
    21541 gtggtcgatg aagcgcatca cctggtgtgg agcgaagatg cgccaagccg tgaatatcag
    21601 gccattgaac aactggcaga gcacgtgccg ggcgttctgc tgctgaccgc gaccccggaa
    21661 cagctgggga tggaaagcca cttcgcccgt ctgcgtctgc tggacccgaa ccgtttccac
    21721 gattttgcgc agttcgttga agagcagaaa aattatcgtc cggttgcgga cgccgttgcc
    21781 atgctgctgg caggtaacaa actgagcaat gacgaactga acatgctcgg cgagatgatc
    21841 ggcgagcagg atatcgagcc gctgttgcag gcagcaaaca gcgacagcga agatgcccag
    21901 agcgcccgtc aggagctggt ttcgatgctg atggatcgcc acggcaccag ccgcgtgctg
    21961 ttccgtaaca cgcgtaacgg tgtgaaagga ttcccgaaac gcgagctgca caccattaag
    22021 ctgccgctac cgacgcagta tcagacggct attaaagtct ccggcattat gggcgcacgt
    22081 aaaagtgcgg aagatcgtgc tcgcgatatg ctctacccgg agcgtattta tcaggaattt
    22141 gaaggtgata acgccacctg gtggaacttc gatccgcgcg ttgagtggct gatgggctac
    22201 ctgaccagcc atcgctctca gaaagtgctg gtgatctgcg ccaaagctgc cactgcgctg
    22261 caactggagc aggtactgcg cgaacgtgaa ggtattcgcg ctgcggtgtt ccacgaaggt
    22321 atgtcgatta tcgaacgtga ccgcgctgcc gcctggtttg ccgaagaaga caccggcgca
    22381 caggtactgc tgtgctcaga aatcggttct gaaggacgta actttcagtt cgccagtcac
    22441 atggtgatgt ttgacctgcc attcaacccg gatctactgg agcagcgtat tggtcgtctg
    22501 gatcgtatcg gccaggcgca cgatattcag atccatgtgc cttatctgga gaaaaccgct
    22561 cagtcggtgc tggtgcgctg gtatcacgaa ggtctggatg catttgagca cacctgcccg
    22621 accggacgca ctatttacga tagcgtatac aacgatctga ttaactatct ggcttcaccg
    22681 gatcaaaccg aaggctttga cgatctgatc aaaagctgcc gcgagcaaca tgaagcgctg
    22741 aaagcacagc tggaacaggg tcgtgaccgc ctgctggaaa tccactccaa cggtggcgaa
    22801 aaagcccagg cactggcaga aagcattgaa gagcaggatg acgataccaa cctgatcgcc
    22861 ttcgccatga acctgttcga tattatcggt atcaatcagg acgatcgcgg cgacaacatg
    22921 atcgtgctga cgccgtccga tcatatgctg gtgccggact tccctggcct gtcggaagat
    22981 ggcatcacca tcacctttga tcgtgaagtg gcgctggcgc gtgaagatgc acagtttatt
    23041 acctgggagc atccgctgat ccgcaacggt ctggatctga tcctttctgg cgataccggt
    23101 agcagcacga tttcactgtt aaaaaacaaa gcgttgccgg taggtacgct gttggtggaa
    23161 ctgatttatg tggttgaagc ccaggctccg aagcagttgc agctcaaccg cttcctgcca
    23221 ccgacgccgg tacgtatgct gctggataaa aacggcaaca acctggcggc gcaggtagag
    23281 tttgaaacct ttaaccgcca gcttaacgcg gttaaccgtc acaccggcag caaactggtt
    23341 aacgccgtgc agcaggatgt tcacgctatc cttcaactgg gtgaagcgca gatcgagaaa
    23401 tctgcccgtg cattgattga tgcagcgcgt aacgaagccg acgaaaaact gtctgccgag
    23461 ctgtctcgtc tggaagctct gcgtgcagtg aacccgaaca ttcgtgacga cgaactgacc
    23521 gccattgaga gcaaccgtca gcaggtaatg gaaagcctgg atcaggcagg ttggcgtctg
    23581 gatgccctgc gtttgatcgt tgtaacgcat cagtaacgga gccgaaaatg gggatggaaa
    23641 actacaatcc accgcaggaa ccctggttgg ttatcctgta tcaggatgac catattatgg
    23701 tggtcaacaa gccgagcggt ttgttgtcag tgccgggtcg tctggaagag cacaaagaca
    23761 gcgtgatgac gcgcattcag cgtgattatc cgcaggcaga atcggtgcat cgtctggata
    23821 tggctaccag cggcgtgatt gtagtggcgc tgaccaaagc cgcggagcgg gagttaaaac
    23881 gccagttccg cgagcgcgag ccgaaaaagc agtatgtggc ccgcgtctgg gggcatccat
    23941 cccccgcaga aggtctggtg gatctgccgc tgatttgcga ctggccaaac cgcccgaaac
    24001 agaaagtctg ttacgaaacg ggtaaacctg cgcagacgga atatgaagtg gtggagtatg
    24061 cggcggataa cacggcaaga gtggtgttaa aaccgattac cgggcgttcg catcaattgc
    24121 gtgtgcatat gctggcgctg gggcatccga ttctcggcga tcgtttttat gcatcaccag
    24181 aagcgagagc gatggcaccg cgtttgttgc tacatgcaga gatgttgacg attacccatc
    24241 cggcgtatgg caatagtatg acgtttaaag cgccagcgga tttttaatcg tcacgctggc
    24301 gcagttcggt aggcggataa ggcgttcacg ccgcatccgg caattgtgcg ccgattgcct
    24361 gatgcgacgc gggccgcgtc ttatcaggcc tacagtttaa aacttattta aaccctttct
    24421 gctgctttat cagctcatat gcctgctgaa tttcctgcgc tttctgcttc gccatctcca
    24481 tcatctcagg cggcaaacct ttcgccacca gcttatcggg atggtgttca ctcatcagct
    24541 tacggtaggc acgtttgatg gtggtcgcat catccgtcgg cttcacgccc agcacattac
    24601 aggcatcttc cagcgtcggg ccacgctgcg cttgctgcca gttaccaccg ccagattgct
    24661 gctgataacc gccgccaaac tgtgcaccgc cctgcatcat gcgcaaaaac tggtcaaact
    24721 gggcgcggga gatccctaat tcttcagcaa tgacatacag caccgcccgt tcattcgggt
    24781 gcagtgaacc atcagcaaac gccgcctgaa tctggatctc cagaaacata cgaattaagt
    24841 caaaacgacc aaagcagaca ctgcgaaact ggcgcatctt ttcgcgcagc gggtaattat
    24901 ctgattttcc cacccggaac gcgttttgcg ccgcagtacg ggaagcgcca tgaaggttca
    24961 ttcggtccat caactggctg gcgatatgaa tatcagcctc cgtgacgcga cctttggatt
    25021 tggttaaatg ccccatcact tcaaaagtgg tggcaaaaaa cagcgcctga cgctcacgct
    25081 ggttggcgaa ccacgccatt ttacggctac gggctttatc aaacatatgg ccaattaaca
    25141 ggcctaacac tacgccccaa aagccgccgc ccatcagtaa ggccacggcc acgccaatga
    25201 tttttcccca atactgcata tattccccaa atcgacacac ggatatcagg gctatctccc
    25261 acaatataaa ggtgctttta ccgttttccg gctgcggtca attgtgggac atcgcctata
    25321 atttgcatta tcatacctgt cattcaatgc cgtgcctaac accactgacg ctattcggac
    25381 aggattaaca ctagcgtaga gatgacgagt acgttagtct ctgaccgttt gtcacgcgca
    25441 acgttaccga tgatggaaca ataaaatcaa cgtatgaaaa aacgtatccc cactctcctg
    25501 gccaccatga ttgccaccgc cctttatagt caacagggac tggcagccga cctcgcctca
    25561 cagtgcatgt tgggcgtgcc aagctatgac cgtcctctgg tacagggcga taccaatgac
    25621 ttacccgtga ctatcaatgc tgaccacgcg aaaggggact acccggatga cgccgtgttt
    25681 actggcagcg tggatatcat gcagggtaac agccgtctgc aggccgacga agtgcagctc
    25741 catcaaaaag aggcaccagg acaaccggag ccggtacgta ccgttgatgc gctcggtaat
    25801 gtccattacg acgataacca ggtgatcctc aaagggccga aaggctgggc gaatctgaac
    25861 accaaagata ccaacgtctg ggaaggtgat taccagatgg tgggtcgcca gggtcgcggt
    25921 aaagcggacc tgatgaaaca acgtggcgaa aaccgctata ccattctgga taacggtagc
    25981 tttacctcct gtctgccggg ttctgacacc tggagcgtgg taggtagcga aattattcat
    26041 gaccgcgaag aacaagttgc tgagatctgg aacgcccgct ttaaggtggg tccggtaccg
    26101 atcttttata gcccctattt gcagttgccg gtgggtgaca aacgtcgctc tggtttcttg
    26161 atcccgaacg ccaagtacac caccaccaac tactttgagt tctacctgcc atattactgg
    26221 aacatcgcgc caaatatgga tgccaccatc acgccgcatt atatgcatcg tcgtggcaac
    26281 atcatgtggg agaacgaatt ccgctacctc tcccaggcgg gcgctggctt gatggaactg
    26341 gactatctgc cttcagataa agtctatgaa gatgaacacc cgaacgatga cagttcacgt
    26401 cgttggttat tctactggaa ccactccggg gtcatggatc aggtgtggcg tttcaacgtc
    26461 gactacacca aggtcagcga tcctagctac ttcaatgatt tcgataacaa gtacggttcc
    26521 agtactgacg gctacgcaac gcaaaaattc agcgttggct atgcggtgca aaacttcaat
    26581 gccaccgttt caaccaagca gttccaggtt ttcagcgaac agaacaccag tagctactcg
    26641 gcagagccgc agttagacgt taattactac cagaatgatg ttggtccgtt tgatacgcgt
    26701 atttacggcc aggcagtgca ctttgttaac accagagacg acatgcctga agcaacccgt
    26761 gttcacctgg aaccgaccat caatttgccg ctctctaata actggggcag catcaatacc
    26821 gaagcgaagt ttctggcaac ccattatcag caaaccaatc ttgactggta taactccaga
    26881 aacacgacca agctggacga atccgttaac cgcgtaatgc cgcaattcaa agttgacggc
    26941 aaaatggtct ttgaacgcga tatggaaatg ctggctccgg gttataccca aacgctggaa
    27001 ccgcgcgcgc agtatttgta cgtgccgtat cgcgatcaga gcgacatcta taactacgac
    27061 tcgtctctgc tgcaatctga ctactctggc ctgttccggg accggactta cggcggtctt
    27121 gaccgtattg cctccgctaa ccaagtgacg accggtgtca catctcgcat atatgatgat
    27181 gctgccgttg aacgttttaa tatttccgtt ggtcaaatct actatttcac ggagtctcgc
    27241 actggcgatg acaacataac atgggagaat gacgacaaaa cgggctcact ggtgtgggca
    27301 ggcgacacct actggcgtat ctccgagcgt tggggattgc gtggcgggat tcagtacgat
    27361 acacgtctgg ataacgtagc gaccagtaac tccagcattg aataccgtcg ggatgaagac
    27421 cgtctggtac agctgaatta ccattacgcc agcccggaat atattcaggc tacgctgcct
    27481 aagtactatt ccactgctga gcaatataag aatggtattt cgcaggtagg tgctgtcgcc
    27541 agcaggccaa ttgccgatcg ttggtccatt gttggggcct actactacga caccaatgcg
    27601 aacaagcaag ccgactctat gttaggtgtg caatacagct cctgctgcta tgcaattcgc
    27661 gtcggttacg agcggaagct gaacggttgg gataacgata aacaacatgc ggtatatgac
    27721 aacgcaatcg gctttaacat cgaacttcgc ggcctgagct ccaactacgg tctgggtacg
    27781 caagagatgc tgcgttcgaa cattctgccg tatcaaaaca ctttgtgatt tgttgattta
    27841 ccacgtaatc cgcagtgcgg ttaattgaaa tggaaaaagt atgaagaact ggaaaacgct
    27901 gcttctcggt atcgccatga tcgcgaatac cagtttcgct gccccccagg tagtcgataa
    27961 agtcgcagcc gtcgtcaata acggcgtcgt gctggaaagc gacgttgatg gattaatgca
    28021 gtcggtaaaa ctgaacgctg cgcaggcaag gcagcagctt cctgatgacg cgacgctgcg
    28081 ccaccaaatc atggaacgtt tgatcatgga tcaaatcatc ctgcagatgg ggcagaaaat
    28141 gggagtgaaa atctccgatg agcagctgga tcaggcgatt gctaacatcg cgaaacagaa
    28201 caacatgacg ctggatcaga tgcgcagccg tctggcttac gatggtctga actacaacac
    28261 ttatcgtaac cagatccgca aagagatgat tatctctgaa gtgcgtaaca acgaggtgcg
    28321 tcgtcgcatc accatcctgc cacaggaagt cgaatccctg gcgcagcagg tgggtaacca
    28381 aaacgacgcc agcactgagc tgaacctgag ccacatcctg atcccgctgc cggaaaaccc
    28441 gacctctgat caggtgaacg aagcggaaag ccaggcgcgc gccattgtcg atcaggcgcg
    28501 taacggcgct gatttcggta agctggcgat tgctcattct gccgaccagc aggcgctgaa
    28561 cggcggccag atggggtggg gccgtattca ggagttgccg gggatcttcg cccaggcatt
    28621 aagcaccgcg aagaaaggcg acattgttgg cccgattcgt tccggcgttg gcttccatat
    28681 tctgaaagtt aacgacctgc gcggcgaaag caaaaatatc tcggtgaccg aagttcatgc
    28741 tcgccatatt ctgctgaaac cgtcgccgat catgactgac gaacaggccc gcgtgaaact
    28801 ggagcaaatt gctgctgata tcaagagtgg taaaacgact tttgctgccg cagcgaaaga
    28861 gttctcgcag gatccaggtt ctgctaacca gggcggtgat ctcggctggg ctacaccaga
    28921 tattttcgat ccagccttcc gtgacgccct gacccgcctg aacaaaggtc aaatgagtgc
    28981 accggttcac tcttcattcg gctggcattt aatcgaactg ctggataccc gtaatgtcga
    29041 taaaaccgac gctgcgcaga aagatcgtgc ataccgcatg ctgatgaacc gtaagttctc
    29101 ggaagaagca gcaagctgga tgcaggaaca acgtgccagc gcctacgtta aaatcctgag
    29161 caactaatgg ttaaaaccca acgtgttgtg atcactcccg gcgagcccgc cgggattggc
    29221 ccggacttag ttgtccagct tgcacagcgt gagtggccgg tcgaactggt tgtttgtgcc
    29281 gatgccactc tccttaccga ccgggcagcg atgctcggtt tgccgctcac cctccgccct
    29341 tattccccca actcccctgc acaaccgcaa actacgggca cattaacgct acttcctgtc
    29401 gcgctacgtg aatctgtcac tgcggggcag ttagcgattg aaaatgggca ttacgtggtg
    29461 gagacgctgg cgcgagcgtg tgatggctgt ctgaacggtg aatttgctgc gctgatcaca
    29521 ggccccgtgc ataaaggcgt cattaacgac gcaagcattc cgtttaccgg tcataccgag
    29581 tttttcgaag agcgttcgca ggcgaaaaaa gtggtgatga tgctggcgac cgaagaactt
    29641 cgcgtggcgc tggcaacgac gcatttaccg ctgcgcgata tcgcagatgc tatcacccct
    29701 gcacttttgc acgaagtgat tgctattttg catcacgatt tgcgtacaaa atttggtatt
    29761 gccgaaccgc gcattctggt ctgcgggctg aatccgcacg cgggcgaagg cggtcatatg
    29821 ggtacggaag agatagacac cattattccg gtgctcgacg agctgcgggc gcaggggatg
    29881 aaactcaacg ggccgctgcc tgccgatacc ctgtttcagc cgaaatatct cgataacgcc
    29941 gacgccgtgc tggcgatgta ccacgatcag ggtcttcccg tgctaaaata ccagggcttc
    30001 gggcgcggtg tgaacattac gctgggcctg ccctttattc gcacatcagt ggaccacggc
    30061 accgcgcttg aactggcggg acgtggcgaa gccgatgtcg gcagttttat tacggcgctt
    30121 aatctcgcca tcaaaatgat tgttaacacc caatgaataa tcgagtccac cagggccact
    30181 tagcccgtaa acgcttcggg caaaactttc tcaacgatca gttcgtgatc gacagcattg
    30241 tttctgccat taacccgcaa aagggccagg caatggtcga aatcggcccc ggtctggcgg
    30301 cattgaccga gccggtcggc gaacgtctgg accagctgac ggtcatcgaa cttgaccgcg
    30361 atctggcggc acgtctgcaa acgcatccat tcttaggccc gaaactgacg atttatcagc
    30421 aggatgcgat gacctttaac tttggcgaac tggccgagaa aatgggtcag ccgctgcgtg
    30481 ttttcggcaa cctgccttat aacatctcca cgccgttgat gttccatctg tttagctata
    30541 ctgatgccat tgccgacatg cactttatgt tgcaaaaaga ggtggtgaat cgtctggttg
    30601 caggaccgaa cagcaaagcg tatggtcgat taagcgtcat ggcgcaatac tattgcaacg
    30661 tgatcccggt actggaagtt ccgccgtcag cctttacacc accacccaaa gtggattccg
    30721 ccgtcgtgcg cctggtccct catgcaacga tgcctcaccc ggttaaagat attcgcgtgt
    30781 tgagccgcat caccaccgaa gcctttaacc agcgtcgtaa aaccattcgt aacagcctcg
    30841 gcaacctgtt tagcgtcgag gtgttaaccg gaatggggat cgacccggcg atgcgagcgg
    30901 aaaatatctc tgtcgcgcaa tattgccaga tggcgaacta tctggcggag aacgcgcctt
    30961 tgcaggagag ttaacgatga tcaattcgcc ccgagtgtgt attcaggttc aaagcgtcta
    31021 cattgaggca caatcttcac ctgataatga acgttacgtt tttgcttata ccgtaaccat
    31081 acgcaatctg gggcgagcgc cagtgcagtt gttggggcgt tactggctga tcaccaatgg
    31141 caatggccgt gaaaccgaag tccagggcga aggagtggtt ggcgtccagc cacttatcgc
    31201 gcctggcgaa gagtaccagt acaccagcgg tgcaatcatt gaaaccccgc tgggcaccat
    31261 gcagggtcac tacgaaatga tcgatgaaaa tggcgtccct ttcagcatcg acattcccgt
    31321 attccgactc gccgttccca cactcattca ttaaaagaat atggcgacat accttattgg
    31381 cgacgttcat ggttgttacg atgaactgat cgcattgctg cataaagtag aatttacccc
    31441 cgggaaagat accctctggc tgacgggcga tctggtcgcg cgcggcccgg gttcgctgga
    31501 tgttctgcgc tatgtgaaat ccttaggcga cagcgtacgt ctggtgctgg gcaatcacga
    31561 tctgcatctg ctggcggtat ttgccgggat cagccgcaat aaaccgaaag atcgcctgac
    31621 accgctgctg gaagcgccgg atgccgacga gctgcttaac tggctgcgtc gccagcctct
    31681 gctgcaaatc gacgaagaga aaaagctggt gatggcccac gcagggatca cgccgcagtg
    31741 ggatctgcag accgccaaag agtgcgcgcg cgatgtagaa gcggtgctgt cgagtgactc
    31801 ctatcccttc tttcttgacg ctatgtacgg cgatatgcca aataactggt caccggaatt
    31861 gcgggggctg ggaagactgc gttttatcac caacgccttt acccgtatgc gtttttgctt
    31921 cccgaacggt caactggata tgtacagcaa agaatcgccg gaagaagccc ctgccccact
    31981 gaaaccgtgg tttgcgattc ctggccctgt cgctgaagaa tacagcatcg cctttggtca
    32041 ctgggcatcg ctggaaggca aaggtacgcc ggaaggtatt tacgcgctgg ataccggctg
    32101 ctgctggggt gggtcattaa cctgcctgcg ctgggaagat aaacagtatt ttgtccagcc
    32161 gtcgaaccgg cataaggatt tgggcgaagc ggcggcgtct taaacacagc ctgatacagg
    32221 aaggccggat aagacgcgac cggcgtcgca tccggcacta gccgtaaatt ctatacaaac
    32281 ttaccgccgc tccagaatct caaagcaata gctgtgagag ttctgcgcat cggcgtcgtg
    32341 gaattcgctg aataccgatt cccagtcatc cggctcgtaa tccgggaaat gggtgtcgcc
    32401 ttccacttct gcgtcgatat gcgtcagata cagtttttgc gcttttggca ggaactgctc
    32461 ataaacgcga ccgccgccaa tcaccatgat ttctggtacg tcaccacacg ccgcaatggc
    32521 ttcatccacc gatttcaccc acgttacgcg atcgtccgta cccggttgac tgctgaggat
    32581 aatatttttg cgtcctggca acggacgacc gattgattcc caagtatggc ggcccataat
    32641 cacgggttta tttaaggtgt tgcgtttaaa ccaggcgaga tcggcaggca ggttccacgg
    32701 catggcgttt tccatgccga taacgcgatc taccgctaac gccgcaatca gactgatcat
    32761 tgagatttcc cgataaaaaa attgtcgcca ctatacgtaa agcgtaaacc gtcgtcgact
    32821 ggtgcgagga tgatgttgag gaaaatttta tattctgctg gcgagtccac gctctccccc
    32881 tggactcgcc gcattacaat gaaacaaaaa caaacagtta gctgtaaagt gtgatttacg
    32941 tcactcttta ttaagatgag ggtttcgttt ccggttcatc cgccatgttg ccggtatgtt
    33001 taccttcttc tgttccctgc cagccatgtc gttgaattaa tgacagatgt tcgcggtcct
    33061 cggtgatgat ctcacttaac atcgcgctgg tgcgtttata gaccgccgcg cgggctttgg
    33121 tgtcattctc aaccattgcc atctcttcca ccatctgaat attaaagcgg cggaacacat
    33181 cggcacgttc tcgcgcttca tacggcccca gacctaaact ttccagcgcc agacgcccgg
    33241 ttttcagcgc accttcaaag gtttcacgct ccggcttttc aacgcctgcc tgacgcaaac
    33301 gaatgtagtg atcgacatcg cgggcgcggg caataatctg caaatgcggg aaatgttctt
    33361 tcaccatctc cgtcagttgc aggttggttt gcggatcgtc aatagcgtta atcagcactt
    33421 ccgctttcgc cgctccggca gattccagta aatccatccg cgtggcatcg ccataaaaca
    33481 ctttcatacc aaatttacgc agcgtttcga tatggtccgg atcgtgatcg agcaccacca
    33541 ttttcacccc gctggagagc agtaaacgtc cggtaatctg tccaaaacga ccgaatccgg
    33601 cgataatcac gcgcggctgt tcttcatcga tctcatcggc ttcacgcgcc tcctccgtag
    33661 aagattgctc aaggcgattg aggatcacca gcagaatcgg cgttgctgcc atcgacagcg
    33721 ccaccgccag ggtcagcgat ttcgcccact ccggctccag cacattcgcc atctgcgccg
    33781 caccaaatac cacaaaggca aactcactgc cctgccctaa caacaccgca aaccagcgac
    33841 gctgtttatt tggcacctgc aacggtcggg caatcagcca cagcatggcg attttgatga
    33901 tgaggaaacc gagcagcaaa atgacaatgc gcaatgggtt ttcaatcagc gtgccaaagt
    33961 ctatcgacat gccaacaccg atgaaaaaca gccccaacag caaaccttta aacggttcga
    34021 tatcgctttc caacgcatga cggtattccg agcttgccag cagtacgccc gccagaaacg
    34081 cgcccatcgc catcgacaag ccgacctctt ccagcagcaa accaaagcca aacacaagga
    34141 ataacgccac ggcactaaac gcttcccgca agccagagcg ggcaacaaaa cgcagcgccg
    34201 gacgcgtaac atagcgcccc agcaatacca ccagtaccag cgcacccgcc acttttaacg
    34261 ccgagagagc aaatgcgccc atcgtcgtcg aggcactgct cgccgccagt agcgggatca
    34321 tcgccaccag cgggattgcc gcgatatcct ggaacagcag cactgcaaag gcactgcggc
    34381 ccatttgcgt caccatcaga ttgcgttcat tcatcgcctg catggcaatc gccgttgatg
    34441 agagcgccag cgtcataccg atcaattccg cgacctgcca gcgcagccca agtaacatac
    34501 agaacagccc cagcaggcca ccgcaaatca ccatctgcaa tgcgccaccg ccgaacactg
    34561 ccgcacgcag cttccacagc ctttgtggat cgagttcgag gccgataata aacagcatca
    34621 gcaccacccc aatctcggca aagtgcagaa tagattcggc atcggtcacc agtcgcagcc
    34681 cccacgggcc aataatgcag ccggcgatca ggtagccaag taccgatccc agaccaagac
    34741 gtaccgcaat gggtacaatc agcgccgccg atccgaggta gatcagcgcc tgaaccagcg
    34801 tatgactatc catgatgggc ctcctgccat tccagcagac gttgcttata gtggcgcgcc
    34861 tgcccttcga gggtttcgtc gtcacaaata aaggtgcagt gcatggcaaa cggtggcagc
    34921 cagttcagcc cgcagtagat tgccgtcgcc tgtagcggct gcgacagcac atcaaagccc
    34981 ggatgcgcac caatttcaaa atggctttcc ccgccgccgg tcgtcaccgc ccacagcaaa
    35041 tgtttgccat gcagcgccgt gccgccgtga ccgtaagccc agccgtgcga caaaacttta
    35101 tcgatccaaa gtttaagaag cggaggaatg ctgtaccact gcatcgggtg ctgccagacg
    35161 atcagatcgg cgcgagagag cgcctcctgc tcggcggcaa tatcgatatt gaagtcagga
    35221 tagagttgat aaagagagcg aatttcgacg ccttccagcg tccttgcctg ttcaagcatc
    35281 cgtttattcg catgggaatg atgcggatac ggatgcgcat aaatgatgag gatcatagtt
    35341 attagcccgc gtgtttacgc ctttgttttc ccacaaagtg tagtcatata ttcacgcggc
    35401 ttatagtcaa taatattgat tcagaaaaga gagaaatctg aacaaataat cccattctct
    35461 gcccgcggat cgtcatattg ctggtctgcg ccagtgacat tcctcgagtc tccggggcaa
    35521 acgctacgga aatcaacaag ccaaacagcg agatacccgc ccccattagc atcgtgttac
    35581 tgataccgta attattgata aatatcggca gtgcccaggt cgaaacaatg gtgccaatac
    35641 gacttaagga cataatcacg cccacggcag aggcgcggat atccgtcggg aagagttcat
    35701 taggatagag ccactgcaaa ttacccggcc cgccagagaa aaaggcatac accgcaaagg
    35761 ccatcactac cagccagatc cccatatccg ggattagccc caaaaccgcc aacgccagcg
    35821 tcatcatggc aaagctgcca atcaacaatg gacgccgtcc ggcagtgttt agccacagca
    35881 tcggcggaat acagccgagc ataaagaaca ggctaatcac cacattccct agtgccgcgt
    35941 ttttgccaac ccccaatccc aacgaaccaa cgatttgcgg gccaaaggtg taaatggcga
    36001 acattgggat cacctggcag gtccagatgg cggcaacaaa cagaacaaaa ggaaaatggc
    36061 ggcgattaaa cagatcgcga aaacgggttt gctgcggctg ctcttcatcg aaagccaccg
    36121 gttcgccaaa cagttttatc atcatctctt cgcactcttt tactcgccct ttgcgtaata
    36181 accagcgggg agattctggc agttcgaatc gaccaatcaa aatcaacaaa caagggatcg
    36241 ccgcgctacc cagcatccag cgccaaccac cttccacatc ataaagccag tagccgacca
    36301 gatcggcgca ggtcgcgccg acataccaca tcgcggcaat aaagctgatg gaaaacgccc
    36361 gctgacgggt actggagaac tcggtgatca tcgaagtggc gatgggataa tctgcaccga
    36421 tgacaatgcc gatgagtacc cgcatcacca acagttcgac gggggatgaa acacacatcg
    36481 tcgccaccga tatcacgccg atggcgatga tatcaatgag gaacattttg cgccgtccga
    36541 ctttatcgga gatatagcca aacagcgatg tgccaacgaa cagcccggcg agcgttcccg
    36601 cgcccagcaa gccaatccag tcagcgtcca gtttcagcgc cggagtcagt tgctccagcg
    36661 ccacgccaat cattaccagt acataaccat ccagaaacgg accaccgctt ccccacagca
    36721 aaatgcggcg gtgaatagag gagaatttga gatcgtcaaa gtttctggac ggttgcatgg
    36781 gttattcctg tttttttgca atgagagtca gtacggcgtt acgtatcaaa ccaacatcag
    36841 ccgtaacgga actccacacc aaaggtgccg cgcgggtatt cccactgttc cagcgccgac
    36901 cccagcccca gaatgcgaca ggtgccgcac tccagacatc cggcgtaatc gaagcgcaca
    36961 ctgccgtcat cctgcttctt gtacagacct gcggggcacg ctttcaccag cagctccagc
    37021 acctgtttat cagcatcggc cttcacaaca atgtgcggat gctcttcatc gacattgaat
    37081 ttattgacgc ccagtttgac gtccacattg acgggagaag tcataaaacg gtcactcctt
    37141 tcatgccatc cttgatcaga ttgatgaagc ccactttctt gccgtggcgg aggattttct
    37201 tgcgcatcag ttccggcgca ctgccgtcaa tggtgaacag gtcacgcgcc acgcccaccg
    37261 ccagttccgg gttgccgcta aacatgcgtg ggttatcaag gaacgccggt agtttctggt
    37321 acatgcgcat atcgcgcagc gggccactct ccagatgctg acgatattcc gcgagttttt
    37381 gcttactgaa atcgtcgctt ttcatcgctg aaagcacggt ttttgctgca gcttccccgg
    37441 cggcaatcgc cagatccata ccgcgaatgg taaaaccgag gttcatacac attccggcgg
    37501 catcaccggc aatcaatacg ccgtcaccga ccagctccgg cagcatgttg atgcctgctt
    37561 ccggcactac gtgagcggaa tattccacca gcttgccgcc cgcgatcagc ggtgcaacgg
    37621 ccggatgctg tttgaaatct tccagcattt gcggcaccga ttttttcgcg tcatgcagat
    37681 gatgcagacc acaaaccagc cccagcgaca gggtattttc attggtataa aggaagccgc
    37741 cgcccatcag gccatcggtg ggtgatcccg caaacagaca agccgcgccc tgattaccct
    37801 gcaactgaaa acggtcttcg attaccgact tcggtaactc gatcagttcc ttcacgccaa
    37861 ccgccacatc cgtcggttta acgcgttttg ccatccccaa cttttcggca aggatggagt
    37921 tcaccccatc agcaaggagc accgttttcg cttcaatcac atcaccatcg gcttctacac
    37981 cgacgacttt gccatcgcgc tgtacgaggt tatcgacgcg gatcccggta attaactgcg
    38041 cgcccgcttc ttcggcctgc tccatcaacc aggcatcaaa tttactgcgc aaaacggagt
    38101 aagaacgctg ggatggcgag gtttcgtcac cgttgcagta gtccatagtc atcgctgact
    38161 tttccgtcat aaacgcgagt ttttcatggg tgatcaggcg ttctacgggg gcggaatcgg
    38221 cgaaacctgg aataatgtgt tccagactgt gggcatagag acgcccgcca gtaacgttct
    38281 tggcacctgc ggaattgcca cgctcgataa ctaacacttg cgccccttcg cgggcgagca
    38341 ccagtgcggc aaccgaaccg gcaagccctg caccgacgat gatggcgtca aagatatctt
    38401 cggacataag agctccctgt cagcggcaga gccgtaatgc agccctgcca gagtggatca
    38461 acgcgctaaa gctgcggtca gtgccggaag gatcttcacg gcatcgccaa caatgccgta
    38521 atccgcgtac tggaagatcg gcgcattttt atctttattg atggcgaaaa tggtttgcga
    38581 cgcgttagcg ccaaccatgt gctggatcta cccggagatc cccaccgcca ggtacagttc
    38641 aggtttcagc atcaggttgg agataccgac atagcgttcg tgctccatcc atttttcgtt
    38701 ttctgccacc ggacgagaac aggccaactc cgcacctatc gccttgcaaa gctgttctgc
    38761 cagcgcaatg ttctctttgc tgccaatgcc gcgaccgacg ctgaccacca gacgggcttt
    38821 gtcgagatcg acgctgttgc tctggcgcgc ctgggttgcc gtgcgggtaa tcgccacagc
    38881 cggagcctgc cactccacgg tgtgcgtttc gccagtgcgt gacgcgtctg gctgagccgc
    38941 atcgaacgtg ccgctgctga tggtcagtac cgcatacggc gtggcaatgc gttcttcgcc
    39001 aatcgccaga ccgccgtaaa ccatgtgttt cactgttgct ttaccgtcct gtacgctgac
    39061 ggtgctggca tcgttagaca ccgccgcttt aaggcgataa cccagttttg ccgccagtaa
    39121 tttgccgcga cgggtgtttg gcaacagcac cagaccgtca gcgccgtgct ggcgaatagt
    39181 gtcagccatg acaccggcgt aatcttcgat catccgatcg tccggtttgc cgtttaattt
    39241 ccagacatga ttagcgccga gctggattgc ctgtgcgccg tcggcatcat tgaggacaaa
    39301 ggcattgatt tgattagcta aagcctgcgc accgttcatc agttccggca gacgagaggg
    39361 ggtatcgctg aatacccaga cttgagaaaa cgtgttcata gcatcccctg taattaaatg
    39421 actttgcgaa gattttcagc aaatgcggcg atctgttctt cgccgtcgcc ttcaatcacg
    39481 atgcgctgac gctcgcgctg tttcggcgcg gcaacctgtt gttcagacca ggcgtccacg
    39541 gcgttaaaac caatatccgc cgccgaccat acctggactg gctttttcgc cgcgccgaga
    39601 atggctttca tcgaaggaat ttgtggggag ttgatatcag tggaaacagc aacaaccgca
    39661 ggcagcggaa tgcttaaggt ttcggtttca tcttccagtt cgcgctcaac ggtgagggta
    39721 tctgccgtca gggagataat tttgctgacg ccgttaactg ccggaatatt gaggatttcg
    39781 cccaccagca gaccaacctg ctgggcataa aggtcggaag aaccatcgcc acagaggatc
    39841 agatcaaagc ctgctttctg ggcggctgca gccagtgcgc tcgccgtttg ttgcggcagt
    39901 gcctgctcga actggtcatc aatcaccaca atcagttcat ccgggccgcg cgatagcaca
    39961 tctttacgcc ctttggcgtt ggtcagggct ttaccgccca cacttaaggc tgtcacctgc
    40021 gcctctgctg cctgttgctt tagctggcaa gccgcttcaa tagcgttgag atcgtattgg
    40081 cttattttgg catcggcttt gctgaagtct aatgaaccat cagcattatt gaccgcaata
    40141 tcctgttcat caggcacgca cttatagcaa gtaataatct tcattgcatc tccagaaatc
    40201 atgaaggaaa cgtcgttcat atcggtggtg ttaattacag atatcacgcc ctttaatttg
    40261 gaaataaaac tttaatcacc aatattgaaa gtcagatgct atttttttac ccacaaaaga
    40321 atataacaaa ctgattaata accaaaatta acgaaaaacg ccctctgttc actcgaaaca
    40381 aaaatgtgat accaatcaca gaatacagct tattgaatac ccattatgag ttagccatta
    40441 acgcgtccac gaggttaata ataattatat taaatgttaa caaaaataaa acaaacggga
    40501 acgcaaaata aatattcgtt ttcacagtgg aattaactca tgaagaatga aaagagaaaa
    40561 acgggaatag aaccgaaggt tttctttccg ccattaataa tcgtcggcat actttgttgg
    40621 cttacagtca gagatctgga tgcggcgaat gtcgttatta atgctgtatt cagttacgtc
    40681 accaatgtat ggggatgggc atttgaatgg tatatggtgg tgatgctttt cggttggttc
    40741 tggctggtgt ttggcccgta tgccaaaaag cgtttaggta acgaaccgcc agaatttagc
    40801 accgccagtt ggatctttat gatgttcgcc tcctgtacgt ctgctgccgt actgttctgg
    40861 ggatcgattg agatctacta ctacatctcc accccgccgt ttggcttaga accgaactcg
    40921 acaggagcga aagagttggg gctggcttac agcttgttcc actggggacc tctgccgtgg
    40981 gccacttaca gcttcctttc agtcgccttc gcttacttct tctttgtccg caaaatggaa
    41041 gtgattcgcc ccagctcgac actggtgccg ctggcaggtg aaaaacacgc caaagggttg
    41101 ttcggtacca tcgtcgacaa cttctatctc gtcgccttga tcttcgcgat gggtaccagt
    41161 ctgggccttg ccacgccgct ggtgaccgag tgtatgcaat ggttgtttgg cattccgcat
    41221 accctgcaac tggacgctat catcattacc tgctggatta tcctcaacgc catttgcgtc
    41281 gcttgcggtc tgcaaaaagg ggtacgtatc gccagtgacg tgcgtagtta cctgagcttc
    41341 ctgatgctgg gttgggtgtt catcgtcagc ggtgccagct tcatcatgaa ctacttcacc
    41401 gattcggtgg ggatgttgct gatgtatctg ccgcgcatgt tgttctatac cgatcccatc
    41461 gctaaaggcg gcttcccgca gggctggacc gtgttctact gggcatggtg ggtgatttac
    41521 gccatccaga tgagtatctt cctcgcccgc atctcccgtg gtcgtactgt gcgtgaactg
    41581 tgcttcggca tggtgatggg gctgacagca tccacctgga tcctgtggac cgtgctcggt
    41641 agtaacactc tgctgttgat ggataaaaac atcatcaaca ttccaaatct gatcgaacag
    41701 tacggtgtgg cgcgcgccat catcgaaacc tgggccgctc tgccactcag caccgccacc
    41761 atgtggggct tcttcatcct ctgctttatt gccaccgtta cgctggttaa cgcctgctct
    41821 tataccctgg cgatgtctac ttgccgcgaa gtacgcgatg gtgaagaacc accgctgctg
    41881 gtgcgtatcg gttggtcaat tctggttggc attatcggta ttgttctgct ggcgctcggc
    41941 ggcctgaaac cgattcaaac cgccattatc gccggaggat gcccgctgtt cttcgtcaac
    42001 attatggtga cgctctcctt tattaaagac gcgaaacaga actggaaaga ttaattaacc
    42061 cccaaaatat caagaggttg aaagatggat tttaatttaa atgatgagca ggaactgttt
    42121 gtcgccggta tccgcgaact gatggccagc gaaaactggg aggcctattt tgccgagtgc
    42181 gaccgtgaca gcgtctaccc ggaacgtttt gtcaaagcac tggcggatat gggtatcgac
    42241 agtctgctga tccctgaaga gcacggtggt ctggacgcgg ggtttgttac tctcgccgcc
    42301 gtgtggatgg agctgggacg tctgggggca ccaacctatg tactgtacca gctgccgggc
    42361 gggttcaata ccttcctgcg cgaaggcaca caagagcaga tcgacaagat tatggctttc
    42421 cgcggcaccg gtaagcagat gtggaactca gcgattaccg aaccgggcgc gggctccgac
    42481 gtgggtagcc tgaaaacgac ttatacccgt agaaatggta agatttatct taatggtagt
    42541 aagtgtttta ttaccagtag cgcctacacc ccgtacatcg tggtgatggc gcgcgacggg
    42601 gcttctccgg acaaacctgt ctacaccgaa tggtttgttg atatgagcaa accgggcatc
    42661 aaagtgacca aacttgagaa gctcggtctg cgtatggata gctgctgtga aatcaccttt
    42721 gacgatgtgg aactggacga gaaagacatg ttcggtcggg aaggtaacgg ctttaaccgc
    42781 gtcaaagaag agttcgacca tgaacgtttc ctggtagccc tgaccaacta cggtacggcg
    42841 atgtgcgcct ttgaagatgc ggcgcgctac gccaaccagc gcgtgcagtt tggcgaggct
    42901 attggtcgtt tccagttgat tcaggaaaaa ttcgcccaca tggcgatcaa attaaactcc
    42961 atgaaaaaca tgctgtatga agcagcgtgg aaagcagaca acggcaccat cacctctggc
    43021 gatgcagcga tgtgcaaata cttctgcgcc aatgcggcat ttgaagtggt ggatagcgca
    43081 atgcaggtgc tgggcggtgt cgggattgcg ggcaaccacc gcatcagccg cttctggcgt
    43141 gacctgcgtg tagaccgcgt ttccggagga tctgacgaaa tgcagatcct gacgctgggt
    43201 cgtgcggtgc tgaagcaata ccgctaagtc cgtgcgctgc ctgatgcgac gctgacgcgt
    43261 cttatcaggc ctacgggagc acgaatgtaa gccggataag gcgtttacgc cgcatccggc
    43321 agtcatgcgc tacataaatt tttcaggagc taataatgga tcatctaccc atgccgaaat
    43381 tcggaccgct ggccggattg cgcgttgtct tctccggtat cgaaatcgcc ggaccgtttg
    43441 ccggacaaat gttcgcagaa tggggcgcgg aagttatttg gatcgagaac gtcgcctggg
    43501 ccgacaccat tcgcgttcaa ccgaactacc cgcagctctc ccgccgcaat ttgcacgcgc
    43561 tgtcgttaaa tattttcaaa gatgaaggcc gcgaagcgtt tctgaaatta atggaaacca
    43621 ccgatatctt catcgaagcc agtaaaggtc cggcctttgc ccgtcgtggc attaccgatg
    43681 aagtactgtg gcagcacaac ccgaaactgg ttatcgctca cctgtccggt tttggtcagt
    43741 acggcaccga ggagtacacc aatcttccgg cctataacac catcgcccag gccttcagtg
    43801 gttacctgat tcagaacggt gatgttgacc agccaatgcc tgccttcccg tataccgccg
    43861 attacttttc tggcctgacc gccaccacgg cggcgctggc agcactgcat aaagtgcgtg
    43921 aaaccggtaa aggcgaaagt atcgacatcg ccatgtatga agtgatgctg cgtatgggcc
    43981 agtacttcat gatggattac ttcaacggcg gcgaaatgtg cccgcgcatg agcaaaggta
    44041 aagatcccta ctacgccggt tgcggtctgt ataaatgtgc cgacggctac atcgtgatgg
    44101 aactggtggg cattacccaa attgaagagt gctttaaaga tattggcctc gcacatctac
    44161 tcggtacgcc ggaaatcccg gaaggcactc agcttatcca ccgtatcgaa tgcccttacg
    44221 gcccactggt agaagaaaaa ctcgatgcct ggctggcggc acataccatc gcagaagtta
    44281 aagaacgctt tgccgaactg aatatcgcct gtgccaaagt actgaccgta ccggaactgg
    44341 aaagcaatcc acagtatgtt gcccgtgaat caatcactca gtggcaaacg atggatggtc
    44401 gcacctgcaa agggccgaac atcatgccga aattcaaaaa taaccccgga caaatctggc
    44461 gcggaatgcc ctcacatggc atggacacgg ctgccatttt gaaaaatatc ggctacagcg
    44521 aaaacgacat tcaggagttg gtcagcaaag gtctggccaa agttgaggac taaacgcgtg
    44581 gcgtcagtcg gcttgcctga ctagcgcccg cccaataaca aacgaacaaa aatggataga
    44641 ggtgcaatgg atatcattgg cggacaacat ctacgtcaaa tgtgggacga tctggcggac
    44701 gtttacggtc ataaaacggc gctgatttgt gaatccagcg gcggagtcgt taaccggtat
    44761 agttatcttg agttaaatca ggagattaac cgcacggcaa acctgtttta tacgctgggg
    44821 attcgcaaag gcaacaaggt tgcactacat ctcgacaact gcccggaatt tatcttttgc
    44881 tggttcgggc tggcaaaaat tggcgcgatt atggtgccga ttaacgcccg cctgttacgc
    44941 gaggaaagcg agtggatctt gcaaaatagc caggcgtgcc tgctggtgac cagtgcgcaa
    45001 ttttatccta tgtatcaaca gattcagcag gaagatgcca ctcaattgcg gcacatttgc
    45061 ctgacagatg tggcacttcc cgctgatgat ggagtgagtt catttactca actgaaaaat
    45121 caacaacctg ccaccttgtg ctatgcaccg ccgctatcga ctgacgatac ggcggaaatt
    45181 ctcttcacct ccggcaccac ctcccgaccg aaaggtgtgg tgattaccca ttacaacctg
    45241 cgcttcgctg gatattactc cgcctggcag tgtgcactgc gtgacgatga cgtctacatg
    45301 acggtaatgc ctgcgtttca tatcgattgc cagtgtactg cggcgatggc ggcgttttct
    45361 gccggggcca cctttgtgct ggtcgagaaa tacagcgccc gcgccttctg gggacaggcg
    45421 cagaagtacc gcgccaccat taccgaatgt attccgatga tgatccgtac gttgatggtg
    45481 cagccgcctt cagcgaacga tcggcaacac cgcctgcggg aagtgatgtt ttatctcaac
    45541 ttgtcggagc aggaaaaaga cgcattttgt gaacgcttcg gcgttcgctt gctgacgtct
    45601 tatgggatga cggaaaccat tgtgggcatt atcggcgatc gccctagcga taaacgacgc
    45661 tggccgtcga ttggtcgggc ggggttttgc tacgaagcgg agatccgcga cgatcacaat
    45721 cgcccgctcc cggcaggtga gatcggtgaa atctgtatta aaggcgtacc agggaaaacc
    45781 atcttcaaag agtattttct caacccgaaa gccactgcga aagtgctgga agccgacggc
    45841 tggctacata ccggcgatac cggataccgc gacgaagaag gcttttttta tttcgtcgat
    45901 cgccgctgca atatgatcaa acgcggtggc gagaatgtct cctgcgtgga gctggaaaat
    45961 atcatcgcca cccatccgaa aattcaggac atcgtggttg tgggtattaa agattcgatt
    46021 cgcgatgaag ccatcaaagc atttgtggtg ctgaatgaag gtgaaacatt gagcgaagag
    46081 gaattttttc gcttctgcga acaaaatatg gcgaaattta aagtgccctc ttatctggag
    46141 atcagaaaag atctgccacg taattgctcg gggaaaataa ttagaaagaa tctgaaataa
    46201 atgaaacgtc agggaactac cctgccagct aataatcacg ccctaaaaca atatgcattt
    46261 tttgcaggga tgttgtcgtc cctaaaaaag caaaaatgga gaaaaggaat gagtgaatca
    46321 ttacatctga cccgcaatgg atcaattctg gaaattaccc ttgatcgccc aaaagcgaat
    46381 gctattgatg caaaaaccag ctttgaaatg ggcgaagtat ttctaaattt ccgtgacgat
    46441 ccgcaattac gtgtcgccat tattaccggt gccggagaga aattcttttc cgcgggctgg
    46501 gatttaaaag cggcagcaga aggcgaagca ccggatgctg actttggccc gggtggtttt
    46561 gcgggattaa ccgaaatttt caatctcgac aaaccagtta tcgcagctgt gaacggctac
    46621 gcctttggcg gcggtttcga actggcgctg gcggcagatt ttattgtttg tgccgataac
    46681 gccagcttcg ccctgccgga agccaaactg ggcatcgttc ctgacagcgg cggtgtgctg
    46741 cgtctgccga agatcctgcc gcctaccatc gtcaatgaaa tggtgatgac cggcagacga
    46801 atgggcgcag aagaggcgct gcgttggggg atagtcaacc gcgtggttag ccaggcggaa
    46861 ctgatggata acgcccgcga actggctcag cagctggtta acagcgcccc gctggcgatt
    46921 gcggcgctga aagagatcta ccgcaccacc agcgaaatgc cggtagaaga agcgtatcgc
    46981 tatattcgca gcggcgtgtt gaaacactat ccatcggttc tgcattcgga agatgccatt
    47041 gaagggccgc tggcgtttgc cgagaagcgc gatccggtgt ggaaaggacg ttaacaacca
    47101 tgagctatta cgcctttgag gggttaattc cggtggttca cccgacggcg tttgtccatc
    47161 ccagtgccgt cttgattggc gatgtgattg tgggagccgg tgtctacatc ggcccactcg
    47221 cctcactgcg tggtgactac gggcggttga tcgtgcaagc gggagccaat attcaggatg
    47281 gctgcattat gcatggctac tgcgacactg acaccatcgt tggggaaaac ggccatatcg
    47341 ggcatggagc gatcctgcat ggttgtgtga ttggtcgcga tgcattggtc gggatgaaca
    47401 gcgtaattat ggatggcgcg gtcattggcg aagagagcat tgttgccgca atgagctttg
    47461 tcaaagcagg ctttagtggc gagaaacgcc agttgttgat gggtacgcca gcccgcgctg
    47521 tacgcagtgt tagtgacgac gagttacact ggaaacggtt gaataccaaa gagtatcagg
    47581 atcttgtcgg gcgctgtcat gcatcgttac atgaaacgca gccactgagg caaatggagg
    47641 aaaatcgccc ccgtttgcag gggacgacgg atgtgacgcc taaacggtga tcttgccgga
    47701 tgcgacctgg ttgctggata agatgctaca acatcgtatc tggcaagcag gacgcccggt
    47761 atcaacgaga taaaattaac gacacatact ctttgacaac atcatctgcc acttctgctc
    47821 gcggttaagt tccgtgagcg gaacggcagg aatacgcggt gtctttctta cgccgactaa
    47881 tttttcccga ctgttattgc gcaggcgcgc gtaaatttgc tcatcgatat cgaccacttt
    47941 aaccagtcgc tgacactggc atccccgccc ttccagctta ttagggatca tcttaacttc
    48001 gcagcttatt tctgtgactt ccgacagaat ataagtcagg gtattaaccg ccttgctgtg
    48061 ttcaatatcg aaatgaatag agatctctct tgctatcacc caccgatttt cagccatcat
    48121 ccattcggcg attaacaagt agagtggttt ttcaacatat ccttcacaca tacgaactcc
    48181 agtttcacaa attaaaagtc tagctatgac tcgttttgaa gcaattaaac aaggccatat
    48241 taaaattgtg gatatatcga tagtatgcaa cttcaccgtc gataagtgtg agctaaatcc
    48301 tgcttatgtc atcaaaaata tcgattcccc aaaagattta cttaatgggc aaaaaaaaac
    48361 ggtcctcatc agagaaccgt attagttatt cgatcaaatt agcggatgaa aaatatccgc
    48421 cacgacacgc tattatttga tctgtgcgtg catttcctgc accgaaatta ctttttcagt
    48481 cgcatcggca ttcagcgcca tcgcggtagc gaaaccaccg ttcagggtgg tgtcataatg
    48541 cactttatat tgcagcgcac tgcgacggat cacgcgggag tcttcaatcg cacgacggcc
    48601 tgaggtggtg ttgatgatgt aggtatattc gccattcttg atacggtcct gaatgtgcgg
    48661 acggccttca tgcaccttgt ttaccagacg cggattgata cccgcttcgc ccagcacaat
    48721 cgccgtgccg tgggtcgcat ccagctcgaa gccctgtttc agcagttttg ccgccaggtc
    48781 caccacgcgt tctttatcgc cttcgcgcac ggaaagcagc gcacgaccgt gtttcttcat
    48841 ggtggagttg ctgcccagct gcgctttggc gaacgcttca gcgaaggtgc ggcccacgcc
    48901 catgacttcc ccggtagagc gcatttctgg ccctaacagc gggtcaacgc ctgggaattt
    48961 attgaacggc agcaccactt ctttcaccga gtagtacggc ggaataactt ctttggttac
    49021 gccctgctca gccagcgatt tgccagccat cacacgcgcc gccacttttg ccagcggtac
    49081 gccggtggct ttggagacga acggaacagt acgcgccgca cgcgggttta cttcaatcag
    49141 gtaaacttcg ttgtttttca ccgcaaactg cacgttcatc agaccgcgca cctgcaattc
    49201 gaaggccagt ttctgcacct gctggcgcat cacatcctga atttcctgac ttaaggtgta
    49261 cgctggcagc gaacatgcgg agtcaccgga gtgcacaccc gcctgctcga tgtgctccat
    49321 gatgccgcca atcagcacca tttcgccgtc gcagatggca tccacgtcaa cttctactgc
    49381 gtcatcaagg aaatgatcca gcaacactgg cgcatcgtta gacacgctaa ccgccgtctg
    49441 gaagtagcga cgcaggtcag cttcgtcata gacgatttcc atcgcccgac cgccgagaac
    49501 gtaagacgga cgcaccacca gcgggtagcc aatctctttt gccttctcaa ctgccatttc
    49561 aatagcagta acggtggcgt tcgccggttg tttcagtttc agacggtcaa ccgcatgctg
    49621 gaagcgttca cggtcttccg cacggtcgat agcatccggg ctggtgccga taaccggtac
    49681 gccagcggct tccagtgcac gcgccagttt cagcggggtc tgaccgccgt actgaacgat
    49741 aacgcctttc ggcttctcaa tacgcacgat ttccagcaca tcttccagag ttaccggctc
    49801 gaagtagagg cgatcggaag tgtcgtagtc ggtagagacg gtttccgggt tacagttaac
    49861 cataatggtt tcgtaaccgt cttcacgcag cgccagcgag gcgtgtacgc agcagtagtc
    49921 gaattcgata ccctgaccga tacggtttgg accgccgcca agcaccatga ttttttcacg
    49981 gtcggtagac ggattcgctt cgcactcttc ttcataagtg gagtacatgt aagcggtgtc
    50041 ggtggcaaac tctgccgcac aggtatccac gcgcttgtag accgggtgca ggtcatattg
    50101 gtcacgcagc ttacggattt ccgcttcgcg tacgcccgcc agttttgcca agcgcgcatc
    50161 ggcaaagcct ttgcgtttca gctggcgcag gaattcagcg ttcaggccag tgatgcccac
    50221 ttccgccact ttctcttcca gacgcaccag ttcttcaatc tgtaccagga accagcggtc
    50281 aatgttagtc aggttgaaga cgccgtccac agacaggccc gcgcggaaag catcggcgat
    50341 gtaccagata cgctctgcgc ctgcgtcttt cagttcgcga cggattttgg ttaacgcttc
    50401 cgggtcatcc aggctcactt tcgggtcgaa gccagttgcg cccacttcca ggccgcgcag
    50461 cgctttttgc agggattcct gctgcgtgcg accaatcgcc atcacttcgc caaccgattt
    50521 catctgagtg gtcagacggt cgttagcacc ggcgaatttt tcgaagttga agcgaggaat
    50581 tttagtaacc acatagtcga tggacggctc gaaggaggcc ggagtacgtc cgccagtgat
    50641 gtcgttcatc agttcgtcta gggtgtaacc caccgccagt ttcgccgcca ctttagcaat
    50701 cgggaaaccg gtcgctttcg acgccagcgc cgaagaacgg gacacgcgtg ggttcatttc
    50761 gataacaatc aggcgaccgt ttttcgggtt caccgcaaac tggacattgg aaccaccggt
    50821 ttcaacgccg atttcacgca gcaccgccat cgaggcgtta cgcatgattt gatattcttt
    50881 gtcggtcagc gtttgggctg gcgcgacagt gatggagtca ccggtgtgga tgcccatcgc
    50941 atcaaagttt tcgatagagc agacgatgat gcagttgtcg tttttatcac gcaccacttc
    51001 catctcgtac tctttccagc cgatcagcga ctcatcaatc agcaactctt tggtcggaga
    51061 gagatccaga ccgcgggcgc aaatttcttc aaactcttca cggttataag cgataccgcc
    51121 gccgctaccg cccatggtaa aggatgggcg aataatgcac gggaagccca cgtcagcggc
    51181 aacagccagc gcttcttcca tcgtgtgtgc gataccggaa cgcgcggttt ccagaccaat
    51241 tttcttcatc gctacgtcga aacgacggcg gtcttctgct ttatcaatcg catcggcagt
    51301 ggcaccaatc atggtgacac cgaactcttc caacacgccc tgacgttcca gctccagcgc
    51361 gcagttcagc gccgtctgac cgcccatggt tggcagcacc gcgtccgggc gctctttttc
    51421 aataatcttg cgtaccactt cccagtgaat cggctcgatg taggtcgcat cggccatttc
    51481 cgggtcggtc atgatggtcg ccgggttgga gttcaccaga atgacgcggt aaccctcttc
    51541 gcgcagggct ttacatgctt gcgcgccaga gtagtcaaac tcacacgcct gaccgataac
    51601 aatcgggccc gcacccagaa tcaggatact ttttatatct gtacgttttg gcatggctct
    51661 tttactcctg attacttagc ggttttacgg tactgctcaa ttaacttgat aaagtggtcg
    51721 aacaacggcg cggcgtcgtg tggaccaggg ctggcttcag ggtgaccctg gaagctgaac
    51781 gccggcttat cgatgcgatg aatgccctgt aacgtaccgt cgaacaggga tttatgcgtg
    51841 acacgcaggt ttgcaggtaa tgttgcttca tccaccgcaa aaccgtggtt ctgggcggtg
    51901 atcatcacca cgtttttctc cacatcttta accggatggt tgccgccgtg gtgaccaaat
    51961 ttcattttga cagtcttcgc accgctcgcc agcgccagca gctgatgacc gagacagatg
    52021 ccgaataccg gaatatcggt ttcgaggaac ttctggatgg cggtaatggc gtaatcgcac
    52081 ggggccgggt cgccaggacc gttggagagg aagatgccgt ctggattcat tttcagcaca
    52141 tcttccgcag aagtttgcgc cggaacgatg gtcaggcgac agcctctatc caccagcatc
    52201 cgcaggatgt tgcgcttggc accaaaatca taagccacga cgtggaacgg cagctcgtct
    52261 tcttttttcg cttctggcag gccgccggtc aacgtccagc tcccttgtgt ccagctatag
    52321 gcttctgcgg tggtcacttc ttttgccaga tccatgccgt tcagacctgg gaacgcgcgg
    52381 gctttttcta acgccagcgc cgcatccggg ttatcgcctg cgataatgca gccattctgt
    52441 gcgcctttct cgcgcagtaa acgcgtcagc ttacgggtat cgatatcggc aatcgccacg
    52501 atgttatggc gcttcaggta agaagagagg tcttcggtat tacggaagtt gctggcaatc
    52561 agcggcaggt cgcgaatcac cagaccttgt gcatgtacct gagaagattc ttcatcggcg
    52621 tcattagtgc cgacattgcc aatatgggga taagtaagag taacgatttg acgagaatag
    52681 gaaggatcag tgaggatttc ttgataaccg gtcattgaag tattgaaaac gacttcccca
    52741 accgccgaac ctgttgcccc tatggcccga ccgtgaaact gggttccgtc ttccagaacc
    52801 aatagcgctg acttaatcaa aacaccctcc agagaatact cactcacttt atttgcatat
    52861 taattcacag tgatgacgta aatcaatgca aatctgctta ccgacgaatt tctggcaaac
    52921 ggcggcattc tggagatatg ggcgctaaaa gtcaacttaa tggcaggatt ttttatatta
    52981 ttttgtggct catgcatgtt ttacaggcat taagcgacaa aaacatcatc taacctgttc
    53041 tggaaaacgc ttgcgcagca agaagattgc atgaaataaa aacgagcagg aaaaagtgga
    53101 ccaaatggtc aaaatttaca ttaaaacaac aaaaatagaa gacgaaacat gtaatttttg
    53161 gcagttatag aaaatttaaa agcataa
//
LOCUS       DAEBXN010000017        51890 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.017, whole genome shotgun sequence.
ACCESSION   DAEBXN010000017 DAEBXN010000000
VERSION     DAEBXN010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 51890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51890
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.017"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(76..2355)
                     /gene="maeB"
                     /locus_tag="KJL99_000978"
     CDS             complement(76..2355)
                     /gene="maeB"
                     /locus_tag="KJL99_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HBD4435801.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGVQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            2644..3594
                     /gene="tal"
                     /locus_tag="KJL99_000979"
     CDS             2644..3594
                     /gene="tal"
                     /locus_tag="KJL99_000979"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HBD4435802.1"
                     /translation="MNELDGIKQFTTVVADSCDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGCVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     TQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            3614..5617
                     /gene="tkt"
                     /locus_tag="KJL99_000980"
     CDS             3614..5617
                     /gene="tkt"
                     /locus_tag="KJL99_000980"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBD4435803.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFPRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQMEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(5720..6763)
                     /locus_tag="KJL99_000981"
     CDS             complement(5720..6763)
                     /locus_tag="KJL99_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HBD4435804.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQIIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDARPTLWITR"
     gene            complement(6889..7464)
                     /gene="nudK"
                     /locus_tag="KJL99_000982"
     CDS             complement(6889..7464)
                     /gene="nudK"
                     /locus_tag="KJL99_000982"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HBD4435805.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(7532..9511)
                     /gene="aegA"
                     /locus_tag="KJL99_000983"
     CDS             complement(7532..9511)
                     /gene="aegA"
                     /locus_tag="KJL99_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HBD4435806.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKFGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDLLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            9717..11434
                     /gene="narQ"
                     /locus_tag="KJL99_000984"
                     /pseudo
     CDS             9717..11434
                     /gene="narQ"
                     /locus_tag="KJL99_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
     gene            11636..14749
                     /gene="acrD"
                     /locus_tag="KJL99_000985"
     CDS             11636..14749
                     /gene="acrD"
                     /locus_tag="KJL99_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HBD4435807.1"
                     /translation="MANFFIDRPIFAWVLTILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASSANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMLLTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(14848..14907)
                     /gene="ypfM"
                     /locus_tag="KJL99_000986"
     CDS             complement(14848..14907)
                     /gene="ypfM"
                     /locus_tag="KJL99_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HBD4435808.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            15288..15644
                     /locus_tag="KJL99_000987"
     CDS             15288..15644
                     /locus_tag="KJL99_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HBD4435809.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            15648..16775
                     /gene="dapE"
                     /locus_tag="KJL99_000988"
     CDS             15648..16775
                     /gene="dapE"
                     /locus_tag="KJL99_000988"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HBD4435810.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            16803..17003
                     /locus_tag="KJL99_000989"
     CDS             16803..17003
                     /locus_tag="KJL99_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HBD4435811.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(17113..17811)
                     /gene="ypfH"
                     /locus_tag="KJL99_000990"
     CDS             complement(17113..17811)
                     /gene="ypfH"
                     /locus_tag="KJL99_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBD4435812.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDQAL
                     SGGKPGDDDVIEMM"
     gene            complement(17885..19900)
                     /gene="tmcA"
                     /locus_tag="KJL99_000991"
     CDS             complement(17885..19900)
                     /gene="tmcA"
                     /locus_tag="KJL99_000991"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HBD4435813.1"
                     /translation="MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            complement(19915..20778)
                     /locus_tag="KJL99_000992"
     CDS             complement(19915..20778)
                     /locus_tag="KJL99_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HBD4435814.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVIQATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            complement(20946..21659)
                     /gene="purC"
                     /locus_tag="KJL99_000993"
     CDS             complement(20946..21659)
                     /gene="purC"
                     /locus_tag="KJL99_000993"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBD4435815.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            complement(21872..22906)
                     /gene="bamC"
                     /locus_tag="KJL99_000994"
     CDS             complement(21872..22906)
                     /gene="bamC"
                     /locus_tag="KJL99_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HBD4435816.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            complement(22923..23801)
                     /gene="dapA"
                     /locus_tag="KJL99_000995"
     CDS             complement(22923..23801)
                     /gene="dapA"
                     /locus_tag="KJL99_000995"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBD4435817.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            23947..24519
                     /locus_tag="KJL99_000996"
     CDS             23947..24519
                     /locus_tag="KJL99_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HBD4435818.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            24519..24989
                     /gene="bcp"
                     /locus_tag="KJL99_000997"
     CDS             24519..24989
                     /gene="bcp"
                     /locus_tag="KJL99_000997"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HBD4435819.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            25242..25859
                     /gene="hyfA"
                     /locus_tag="KJL99_000998"
     CDS             25242..25859
                     /gene="hyfA"
                     /locus_tag="KJL99_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HBD4435820.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGESPLSLTQEQH"
     gene            25859..27876
                     /gene="hyfB"
                     /locus_tag="KJL99_000999"
                     /pseudo
     CDS             25859..27876
                     /gene="hyfB"
                     /locus_tag="KJL99_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            27887..28834
                     /gene="hyfC"
                     /locus_tag="KJL99_001000"
     CDS             27887..28834
                     /gene="hyfC"
                     /locus_tag="KJL99_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HBD4435821.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQNYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            28851..30290
                     /gene="hyfD"
                     /locus_tag="KJL99_001001"
     CDS             28851..30290
                     /gene="hyfD"
                     /locus_tag="KJL99_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HBD4435822.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMTLVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVDFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            30302..30952
                     /gene="hyfE"
                     /locus_tag="KJL99_001002"
     CDS             30302..30952
                     /gene="hyfE"
                     /locus_tag="KJL99_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HBD4435823.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            30957..32543
                     /gene="hyfF"
                     /locus_tag="KJL99_001003"
     CDS             30957..32543
                     /gene="hyfF"
                     /locus_tag="KJL99_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HBD4435824.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKP
                     PQAVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRS
                     TWHDFLPSGTASVSEKHSER"
     gene            32533..34247
                     /gene="hyfG"
                     /locus_tag="KJL99_001004"
                     /pseudo
     CDS             32533..34247
                     /gene="hyfG"
                     /locus_tag="KJL99_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
     gene            34257..34802
                     /gene="hyfH"
                     /locus_tag="KJL99_001005"
     CDS             34257..34802
                     /gene="hyfH"
                     /locus_tag="KJL99_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HBD4435825.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTH
                     KADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            34799..35557
                     /gene="hyfI"
                     /locus_tag="KJL99_001006"
     CDS             34799..35557
                     /gene="hyfI"
                     /locus_tag="KJL99_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HBD4435826.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKRHLLQDIRRSAYVYRIDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            35550..35963
                     /gene="hyfJ"
                     /locus_tag="KJL99_001007"
     CDS             35550..35963
                     /gene="hyfJ"
                     /locus_tag="KJL99_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HBD4435827.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            35993..37822
                     /gene="hyfR"
                     /locus_tag="KJL99_001008"
     CDS             35993..37822
                     /gene="hyfR"
                     /locus_tag="KJL99_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HBD4435828.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRYAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEAQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PHNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADACRRMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRHDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMA"
     gene            37823..38005
                     /locus_tag="KJL99_001009"
     CDS             37823..38005
                     /locus_tag="KJL99_001009"
                     /inference="COORDINATES: protein motif:HMM:NF014950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HBD4435829.1"
                     /translation="MLHPTTPENDEEERQRIVQVLRETNGIVAGPRGAATRLGMKRTT
                     LLSRMQRLGISVREVL"
     gene            38027..38875
                     /locus_tag="KJL99_001010"
                     /pseudo
     CDS             38027..38875
                     /locus_tag="KJL99_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
     gene            complement(38913..39974)
                     /locus_tag="KJL99_001011"
     CDS             complement(38913..39974)
                     /locus_tag="KJL99_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBD4435830.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPVLMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            40187..41650
                     /gene="bepA"
                     /locus_tag="KJL99_001012"
     CDS             40187..41650
                     /gene="bepA"
                     /locus_tag="KJL99_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HBD4435831.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQKRFKPYTKM"
     gene            41671..42030
                     /gene="arsC"
                     /locus_tag="KJL99_001013"
     CDS             41671..42030
                     /gene="arsC"
                     /locus_tag="KJL99_001013"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708333.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HBD4435832.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIMVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(42163..42909)
                     /gene="hda"
                     /locus_tag="KJL99_001014"
     CDS             complement(42163..42909)
                     /gene="hda"
                     /locus_tag="KJL99_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HBD4435833.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(42959..44248)
                     /gene="uraA"
                     /locus_tag="KJL99_001015"
     CDS             complement(42959..44248)
                     /gene="uraA"
                     /locus_tag="KJL99_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HBD4435834.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPNSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(44334..44960)
                     /gene="upp"
                     /locus_tag="KJL99_001016"
     CDS             complement(44334..44960)
                     /gene="upp"
                     /locus_tag="KJL99_001016"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HBD4435835.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            45284..46321
                     /gene="purM"
                     /locus_tag="KJL99_001017"
     CDS             45284..46321
                     /gene="purM"
                     /locus_tag="KJL99_001017"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HBD4435836.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            46321..46959
                     /gene="purN"
                     /locus_tag="KJL99_001018"
     CDS             46321..46959
                     /gene="purN"
                     /locus_tag="KJL99_001018"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HBD4435837.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            47131..49197
                     /gene="ppk1"
                     /locus_tag="KJL99_001019"
     CDS             47131..49197
                     /gene="ppk1"
                     /locus_tag="KJL99_001019"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HBD4435838.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNAHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRSDSIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            49202..50743
                     /gene="ppx"
                     /locus_tag="KJL99_001020"
     CDS             49202..50743
                     /gene="ppx"
                     /locus_tag="KJL99_001020"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HBD4435839.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEILRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(50782..>51890)
                     /gene="pdeF"
                     /locus_tag="KJL99_001021"
     CDS             complement(50782..>51890)
                     /gene="pdeF"
                     /locus_tag="KJL99_001021"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HBD4435840.1"
                     /translation="KQKLSHWLSPLLEPGEDVYQLSGNDLALRLNTESHQERITALDS
                     HLKQFRFFWDGMPMQPQIGVSYCYVRSPVNHIYLLLGELNTVAELSIVTNAPENMQRC
                     GAMYLQRELKDKVAMMNRLQQALEHNHFFLMAQPITGMRGDVYHEILLRMKGENDELI
                     GPGSFLPVAHEFGLSSSIDMWVIEHTLQFMAENRAKMPAHRFAINLSPTSVCQARFPV
                     EVSQLLAKYQIEAWQLIFEVTESNALTNVKQAQITLQHLQELGCQIAIDDFGTGYASY
                     ARLKNVNADLLKIDGSFISNIVSNSLDYQIVASICHLARMKKMRVVAEYVENEEIREA
                     VLSLGIDYMQGYLIGKPQPLIDTLNEIEPIRESA"
BASE COUNT        12084 a        13715 c        13487 g        12604 t
ORIGIN      
        1 ataagctttg ctaatatatt tcaggaaaac cgcttaccct gaatattcag ggtaagcgtg
       61 agagttaaga atgaattaca gcggttgggt ttgcgcttca accacggcca gcgccaccat
      121 attgacgata cgacgcaccg atgcgatcgg cgttaacacg tgaaccggtt tcgccacgcc
      181 catcagcacc ggaccgacag tcacaccttc cgagctggaa acgcgcagta agttgtaact
      241 aatgcgggca gcttccatgt tcggcatcac cagaatattg gcagaacctt tcaaagggct
      301 gtccggcata cggtcgttgc gaatcgcttc caccagcgct gcatcgccgt gcatttcacc
      361 atcaatcatc agatctggcg cacgatccct gaccagttcc agcgcctgac gcattttgct
      421 cgacgacggg cagtcagaag aaccaaagtt ggagtgcgac aacagagcaa cgcgcggctc
      481 aataccaaaa cgacggacag tttctgccgc catcaaggtg atctccgcca gctcttctgc
      541 gtctggttcg tcattgacgt aggtatcggc aataaaggtg ttaccactcg gcagcagcag
      601 cgcattcatt gcacctgcgg tgtgaacgcc atcgcgataa ccaaagacat ttttcaccac
      661 actaaaatgt tcatgataat cgcccaccgt accgcagatc attgcatcgg cttccccacg
      721 ctgaaccatg atcgcgccga tcactgtcgg gttactgatc agcgcacgct gcgcctgttc
      781 ctgagtgaca ccgcgacgct tcatgatctg gaagtattcg gtccagtact ctttaaagcg
      841 cggatcggat tcgttattga cgatttcaaa atcaacgccc gctttgatct gcaagcccag
      901 tttctgaatg cgcatttcga tcacgttcgg acgaccgata aggatcggtt tcgccagtcc
      961 cagcgttacc agttcctgag tcgcatgcag aacgcgcgcc tcttcccctt ccggcagaac
     1021 tacgcgcttc ggcgctttgc gagcctggga gaaaatcggc ttcataaaca ggttggtttt
     1081 gtaaacgaac tcagtcagct tgtcgatgta gacgtcgaaa tcagcaatcg gacgcgtcgc
     1141 cacgcccgac tccatcgccg cttttgcgac cgcaggagcg atcttaacga tcaagcgcgg
     1201 atcaaacggt tttggaatga tgtattccgg accaaagctc agatcctgaa cgccatacgc
     1261 tgaagccacc acttcactct gttctgcatg ggccagttct gcaatcgcgc gtaccgccgc
     1321 cagtttcatc tcttcgttga tggcggttgc gccaacgtcc agcgcgccac ggaagatgaa
     1381 cgggaagcaa agaacgttgt tcacctggtt cgggtagtcg gaacggccgg tacagatgat
     1441 ggcatccgga cgcacttctt tcgccagcgg cggcagaatt tccggttccg ggttcgccag
     1501 cgccaggatc attggtgccc gagccatttt cttcaccatt tcctgggtca gcactttcgg
     1561 gccggaacag cccaggaaaa tatccgcacc ttcaatcaca tcatcgaggg tacgtttgcc
     1621 gtcatccacc accgcatatg cggctttggt ttccgccata tttggctcac ggccctgata
     1681 gataacgcct tttgaatcgc aaaccacgat gttatgtttt tgcagcccca gcgctaccag
     1741 caggttcata caggcgattg ctgcggcacc cgcgccggaa accaccatcc gcacgtcgga
     1801 gatgtttttc tccaccacgc gcaagccgtt gaggatggcg gcagtgctga tgattgccgt
     1861 gccgtgctga tcgtcgtgga ataccggaat attcatccgc tcgcgcagtt tctgttcaat
     1921 atagaaacat tctggcgctt taatgtcttc gaggttgatg ccgccgaagg tcggttcgag
     1981 cgcggcgacg acttcaataa atttgtccgg gtcgagttca tcaacttcaa tgtcaaatac
     2041 atcaatccca gcgaatttct taaatagaac gcccttgcct tccatcaccg gtttgcctgc
     2101 cagcgcgcca atgttgccta accccagcac cgccgtaccg ttagagatca ccgcaaccag
     2161 gttaccgcgc gcggtatatt tgtaggcttt tagcgggtct ttttcgattt caagacaagg
     2221 tgcggcaacg cctggtgagt aggccagcgc cagatcgcgc tgcgttgcca gaggcttggt
     2281 tggagaaacc tggattttcc ctggaactgg aaattcatgg aaatcaagtg cactttgttt
     2341 taactggtca tccatttggt tgttcctttc acgtaacgtt cacaaataaa gtgtgtgggc
     2401 aacagcccct gcccacaacg tggcgcacat tattaccctg ccggagtcta cagactttga
     2461 gcaagtccaa actctcacca ttaatataat gttttggtaa taatcctata acactgatgt
     2521 tacctgctta atccagcaat accatgcctg tctgctatgc ttttttgatg cgtttagcga
     2581 aatttctcag aagtgtgaat taacgcactc atctaacact ttacttttca aggagtattt
     2641 cctatgaacg agttagacgg catcaaacag ttcaccactg tcgtggcaga cagctgcgat
     2701 attgagtcca ttcgccatta tcatccccag gatgccacca ctaatccttc gctgttactc
     2761 aaggctgccg gattatcaca atatgagcat ttaatagacg atgctatcgc ctggggtaaa
     2821 aaaaatggca agacccagga acaacaggtg gtcgcagcgt gtgacaaact ggcggtcaat
     2881 ttcggtgctg aaattctcaa aatcgtaccc ggttgcgtgt caacagaagt tgatgcacgc
     2941 ctctcttttg ataaagaaaa gagtattgag aaggcgcgcc atctggtgga cttgtatcag
     3001 caacaaggcg ttgagaaatc acgcattttg atcaaactgg cttcgacctg ggaaggaatt
     3061 cgcgcggccg aagagctgga aaaagaaggt attaactgca acctgacgct gctgttttct
     3121 tttacccagg cgcgggcctg tgcggaagca ggcgtttttc tgatttcgcc gtttgtaggg
     3181 cgtatttatg actggtatca ggcacgcaag ccgatggacc cgtatgtggt ggaagaagat
     3241 ccgggcgtta aatcggtgcg caatatctac gactactata agcaacacca ctatgaaacc
     3301 attgtgatgg gcgcgagctt ccgtcgcacc gaacaaatcc tcgccttaac cggctgcgat
     3361 cgactgacta tcgcaccgaa tttgctgaag gagctgcagg aaaaagtttc tccagtggta
     3421 cgtaaattaa tcccgccttc tcagacgttc ccacgcccag cacccatgag cgaagcggag
     3481 ttccgttggg agcacaatca ggatgcgatg gcggtagaaa aactgtctga aggcattcgt
     3541 ctgttcgctg ttgatcaacg caaactggaa gatcttcttg ccgccaaact ataaaccagc
     3601 cacggagtgt tatatgtccc gaaaagacct tgccaatgcg attcgcgcac tcagtatgga
     3661 tgctgtacaa aaagccaatt ctggtcatcc cggcgcgccg atgggcatgg ctgatattgc
     3721 cgaagtgctg tggaacgatt ttcttaaaca taatcctacc gacccaacct ggtatgatcg
     3781 cgaccgcttt attctttcca acggtcacgc gtcgatgctg ctctacagtt tgctacatct
     3841 gaccggttac gacctgccgc tggaagaact gaagaacttc cgtcagttgc attcgaaaac
     3901 cccaggccac ccggagattg gctatacgcc aggcgttgaa accaccaccg gcccgcttgg
     3961 acaaggtttg gcgaacgccg tcgggctggc gatagcagag cgtacactgg cggcgcagtt
     4021 taaccagcca gaccatgaga tcgtcgatca cttcacctat gtgtttatgg gcgacggctg
     4081 cctgatggaa ggtatttccc acgaagtctg ttcgctggca ggcacgctgg gactaggcaa
     4141 gctgattggt ttttacgatc acaacggtat ttccatcgac ggtgaaacag aaggctggtt
     4201 taccgacgat acggcaaaac gttttgaagc ctatcactgg catgtgatcc atgaaatcga
     4261 cggtcacgat ccgcaggcgg tgaaggaagc gatccttgaa gcgcaaagcg tgaaagataa
     4321 gccgtcgctg attatctgcc gtacggtgat tggctttggt tcgccgaata aagcaggtaa
     4381 ggaagaggcg cacggcgcac cactggggga agaagaagtg gcgctggcac ggcaaaaact
     4441 gggctggcac catccgccat ttgagatccc taaagagatt tatcacgcct gggatgcccg
     4501 tgaaaaaggc gaaaaagcgc agcagagctg gaatgagaag tttgccgcct ataaaaaggc
     4561 tcatccgcaa ctggcagaag agtttccccg acggatgagc ggtggtttac cgaaggactg
     4621 ggagaaaacg actcagaaat atatcaatga gttacaggca aatccggcga aaatcgctac
     4681 ccgtaaggct tcgcaaaata cgcttaacgc ttacgggccg atgctgcctg agttgctcgg
     4741 cggttcggcg gatctggctc ccagcaacct gaccatctgg aaaggttctg tttcgctgaa
     4801 ggaagatccg gcgggcaact acattcacta cggggtgcgt gaatttggca tgaccgctat
     4861 cgccaacggc atcgcgcacc acggcggctt tgtgccgtat accgcgacgt tcctgatgtt
     4921 tgttgaatac gcccgtaacg ccgcgcggat ggcggcactg atgaaagcgc ggcagattat
     4981 ggtttatacc cacgactcaa ttggcctggg cgaagatggt ccgacgcacc aggctgttga
     5041 gcaactggcc agcctgcgct taacgccaaa tttcagcacc tggcgaccgt gcgatcagat
     5101 ggaagcggcg gtgggctgga agctggcggt tgagcgccac aacggaccga cggcactgat
     5161 cctctcaagg cagaatctgg cccaggtgga acgtacgccg gatcaggtta aagagattgc
     5221 tcgtggcggt tatgtgctga aagacagcgg cggtaagcca gatattattc tgattgccac
     5281 cggttcagag atggaaatta ccctgcaagc ggcagagaaa ttagcaggag aaggtcgcaa
     5341 tgtacgcgta gtttccctgc cctcgaccga tattttcgac gcccaggatg aggaatatcg
     5401 ggagtcggtg ttgccttcta acgttgcggc tcgcgtggcg gtggaagcag gtattgccga
     5461 ttactggtac aagtatgttg gtctgaaagg ggcaattgtc gggatgacgg gttacgggga
     5521 atctgctccg gcggataagc tgttcccgtt ctttggcttt accgccgaga atattgtggc
     5581 aaaagcgcat aaggtgctgg gagtgaaagg tgcctgatgg tgatcgccgg atgctgtatg
     5641 ttcccctcac cctaaccctc tccccagaag ggcgagggga cggtccgtgc gctttttagg
     5701 gccgcatctg cgattttgtt taacgggtga tccacaacgt gggcctggcg tctggcccat
     5761 gccagttatc gcaggtaggt tctgcggcgt aacgcaccag gcgaaaacgc tgaccgtcaa
     5821 agcgccagcg cgcctgaatg ccacaatcgc ttaatccgcg ccctttcgct aaggtcacca
     5881 gttcacgcga tttctcatca aatgttgcgt tcatcagttc cagttcattc gtctcctggc
     5941 cgttgttgaa cggcaaacgc aaccgaaccg ggcgcgaggc tagcggcttt ttacgcgaca
     6001 caatccatgc caaatcaatg gtgttatagg cccctgcctc acagctaatc atcatcagtg
     6061 ctttatcatc ggtcagcgca gtgacattca cctcacgacg taacggatca agcgagcagc
     6121 gcagaccatt catccgccag tttccataat ccagcaaatc gttacgttct tcgagtgaga
     6181 gtggcgtcgg cgttgggtta accaccgcga cctctttcag cgcaggcgcg ggcggtacgc
     6241 tgagaggcgg ttcgtcccct ttcttgatcc acgcggtttc actgccaacg cgcttttgct
     6301 gagcatcaat aaacaacaac gccgctttta agccgctcag agaaatggtc tgattgccgt
     6361 cgcgtaaggt gatcgccttc ccttcctgaa ttatctgcaa aaacgcggtg atggttgccg
     6421 tatcatcggt tactaataac catggtgaaa tccgccactt gtcaccactt aatgccaacg
     6481 gctcaccatc taatagcagc cgtggcgcta tctccccttc agacgcatcc ggcgacttca
     6541 acccaccgcg ctcaatacgt aaaacggcat cggtatgcgc cccagcgctg cggctcaggg
     6601 tcatcaccag tccattatga tcgcccgtat tgcgcgccac acaaaaattt tgattattac
     6661 aggtgacctg ccagtcggaa aacgcccgtt gcgctggtgc cgcccacacc aaagacgtcg
     6721 gcaacaaagc gaaaaaaaag agaaggaaaa tgcgatagcg catggacggt acgaccccag
     6781 aagttcaaac aaacagcaca agtcgtatct tcgttgcgca ggtagcgcgg ctcaatcgga
     6841 tttgtcggat tgagccaata ataaacaacg aaatattatt gctatttttc agtccattaa
     6901 atgtgacatt tgcaaatagt taagcaataa caccgtctta ccgtcacgta tctcgccggt
     6961 tttgatcatc tccagcgcct ggctgaacgg cagctcgagc acttcaatat cttcatcttc
     7021 aacaccgcca ccggcgttag cgcgctgatt gtcactgtat tcggcgataa aaaagtggat
     7081 tagctcagtc acaccgccag gcgacatata cagttcaaat aatttgcgca cttcgcccac
     7141 ttcatagccc gtctcttcaa tcgcttcttt acgaatgcac acttccggtt cgtcgttatc
     7201 cagcagcccg gcgcaggttt caatcagctg cccgctttca ttgccattaa cccaggtagc
     7261 gacacggaac tgacgaatca gaaccacgct ctttttcttc gcgttgtaca ggaggatcgt
     7321 cgcaccatta ccgcgatcgt aaacttcacg tttatggcgg ataacttcgc catctttgcg
     7381 cgtgagatcg taagtaatgt tgtgcagggt gaaatagtta tcggagagaa ttttgtcttt
     7441 aatgagggtg atttgttgcg tcataccgac tccacagcgc gaaatgaaca attatattac
     7501 gctgtgaagt cgggtttgtc tgcgcaggct atcagtgaga tttgactgat tttaccccca
     7561 gccagtcaat aatcccctgt gccgcatgac gtccttctgc cattgcggta accaccagat
     7621 ccgcaccacg cacggcgtcg ccaccggcga agattttcgg attggtggtc tggtaacggt
     7681 actggctttc cacatccgcg atgatgcggc cccatttgtc taccgttaca ccgtgcgact
     7741 ccagccacgg catcccgtgt ggattgaagc caaacgccat aatcaccgca tcggctggca
     7801 tgacaaattc actgccctca atcggtacag gtcgccgacg cccctgggca tccggctcgc
     7861 caagacgcgt gcgcaggaaa cgaattccgc aaacgtgacc ttgctcattc agctcaagcg
     7921 ccaccggctg gacgttaaat tcgaagttgg ccccctcttc gcgggcgttc ttcacctctt
     7981 tcttcgagcc tggcatgtta gcttcatcac gacgataagc gcaggtgacg ttactcgcgc
     8041 cgtggcgcag cgcagtacgc acacagtcca tcgcggtgtc gccgccgccc agtaccacga
     8101 cgttaagtcc ggcggtattg ataaacggct cttccggtag ctcttcgagc cccatcacct
     8161 gtttagtgtt ggcaatgagg aacggcagcg cgtcataaac gcccggcgca tcttcattgg
     8221 gtaaacccgc tttcatggaa cggtaagtgc ctacgccaac gaagaccgcg tcgtattgtt
     8281 ccaaaagcaa atccaaagag acatctttac ccacttcaca attgagttcg aagtgaatcc
     8341 ccatcgcgct gaagatttcc cgacggcgtg ccagcaggga tttatccagt ttgaaagaag
     8401 gaatgccgaa agtgagcaag ccaccgattt ctggatggcg atcgtacacc gtcaccgcca
     8461 cgccattgcg ggtcagaaca tccgcacagg ccagccctgc cggacctgcc ccgataatcg
     8521 ccacccgctt gtccactttg gtgacatgac ttaagtcagg acgccaacct ttcgccaacg
     8581 cctgatctga aatatagcgt tcaatgttgc cgatagttac cgcgccgtgc tcatcgcgaa
     8641 tagtacaggc accttcacac aaacggtctt gcggacaaac gcgtccggta atttccggca
     8701 gggtgttggt ctggtgagaa agctcgacgg cggcgtcgat gtttccggct ttcaccagtt
     8761 caatccactg cggaatatgg ttatgcagcg ggcaggtcca ttcacaaacg ctgtgctcgc
     8821 cgaacttaag gcagcgcgag gcttcccgtt gtgcctggtc ggcgcgaaat ggcagataaa
     8881 tttcatcaaa accggttttg cgcgcttcaa tcgccagttt atccggctcg ccacgcgcgg
     8941 gcgttgcctg catttgttcg actttactca ttaccggcat ttcttgtgca gcggtactgg
     9001 catgccacgg ttgatgctcc tgacgtgcgg tgcgcaagcg gcgggatttc gccatgccgg
     9061 agagtgcgac gtcagtgacc agttgcagcg cgtccgccgg gcaattctcg acacaggcag
     9121 gaccgttttc gcgccctgca caaaggtcgc atttatgcgc cgtggctttt acttttcctg
     9181 ccgcgacggg tgtcaggacg atttgcatcg taccaaacgg acaggccacc acgcaggatt
     9241 tacagccaat acacttttgc tgattgacct gaatgctgtc atcaacgtgg ctgattgcgc
     9301 cattagggca gctacgggcg cagggcgcat cttcacaatg gtgacaggtc actgcactac
     9361 gttgctgctg atgtttgata accgtaattc ggggatgaaa atggtgttgg ctcaggacat
     9421 gttgctcatc attgtgagcc atgacacagg cgatttcaca agcatgacaa cccagacatt
     9481 gctgactgtt ggccataata aaacgattca taacgacctt cttttttggt tgtaaaaacc
     9541 ttattcttta tatgagtgtt gttattaccc gacttacagg ggaaccggca atgttcatgt
     9601 gcccagaata agtaactatt tcgctataaa ctgtggcaga tcaaataatc ccatcactga
     9661 ctaaattgcg tttcagcgaa ctggaacatt aatgattttt tgtggagaag acgcgtgtga
     9721 ttgttaaacg acccgtctcg gccagtctgg cccgggcctt tttttacatt gtgctgctgt
     9781 cgattctttc cacgggtatc gctctgctaa ctctggcgag cagtttgcgc gacgctgagg
     9841 ctatcaatat tgccggatcg ctgcgtatgc agagttaccg cctgggctac gacttgcaaa
     9901 gtggcagtcc acaactcaat gcacatcgcc agttgtttca gcaggcactg cattcaccgg
     9961 tattaaccaa cctcaacgtc tggtatgtgc cagaagcagt aaaaactcgc tatgcgcatc
    10021 tgaatgccaa ctggctggag atgaataatc ggctcagcaa gggcgatttg ccgtggtatc
    10081 aggccaatat taataattat gttaatcaga tagacctgtt cgtgctggct ttacagcact
    10141 acgctgaacg caaaatgctg ctggtggtgg cgatttccct ggctggcggt atcggtattt
    10201 tcacgctggt cttttttact ttgcgccgca tacgccatca ggtggttgcc ccgctgaatc
    10261 agctggttac cgccagtcag cgtattgaac acgggcagtt cgactcgccg ccgctggata
    10321 ccagtctgcc gaatgagctt ggtctgcttg caaaaacctt taaccaaatg tcgagcgagc
    10381 tgcataaatt gtacctttcg ctggaggcgt cagtagaaga aaagacccgc gatctccacg
    10441 agaccaagcg tcgtctggag gtgttgtatc agtgcagtgt tcccaggcgc tgaacaccag
    10501 ccagattgat gtgcattgtt tccgccatat tttgcagatt gttcgcgaca atgaagcagc
    10561 tgaatatctg gagctgaacg ttggggataa ctggcggatt agcgaaggga agccaaaccc
    10621 tgagctgcca atgcagattt taccggtgac aatgcaagag acggtttacg gcgaactgca
    10681 ctggcaaaat agtcacgttt catcatcaga accgctgctt aacagtgttt catcgatgct
    10741 gggacgcggt ttgtacttta atcaggcgca gaagcatttt cagcagttat tgttgatgga
    10801 agaacgtgcg accatcgccc gcgaattgca cgactcgctg gctcaggtgc tttcttactt
    10861 acgtatccag ttgacgttac tgaagcgttc gataccggaa gataacgcca ccgcacaaag
    10921 tatcatggcc gatttttccc aggcgttgaa tgatgcttat cggcagttac gcgagctgtt
    10981 gactactttc cgcctgacgc tgcagcaggc ggatctcccc tccgcgttga gggaaatgct
    11041 ggatacgtta caaaatcaaa ccagcgctaa actgaccctc gactgccgtc tgccaaccct
    11101 ggcactggat gcgcaaatgc aggtgcattt gttgcaaatt attcgcgaag cggtgctgaa
    11161 tgcgatgaag cacgccaacg ccagcgaaat cgccgtcagt tgcgtcaccg cgccggacgg
    11221 caatcacacg gtttatatcc gtgataacgg gattggtatc ggtgaaccga aagaacccga
    11281 aggtcattat ggactgaata tcatgcgcga acgcgcggaa cggctgggcg ggacgctgac
    11341 tttttcacaa ccttccggcg gcggcacgtt agtgagtatt agctttcgct ccgcagaggg
    11401 tgaggggaag gggaacacgc cggctgaggt gtaaataatt accctcacac cggccctctc
    11461 cctttaaagg cgagagggaa aaacgtgccc atattgacct gtaagcgagt gtgtcagcaa
    11521 tctcatgata atttacatta actccttttt ttctccacga ttggctcgta ccttgccgct
    11581 acagtgaagc aagtcaagcc tacaacaata cgcagaaaca cgaggtcctc ttttaatggc
    11641 gaatttcttt attgatcgcc ccatttttgc ctgggtgctg acaatcctgt tgtgtctgac
    11701 aggtaccctg gcgatttttt cattgcccgt tgaacaatac cccgatctcg cgccaccgaa
    11761 tgtgcgagtg accgctaact atcccggcgc atcggcccag acgctggaaa acaccgtgac
    11821 ccaggttatc gagcaaaata tgaccggcct cgataatctg atgtatatgt catctcagag
    11881 cagtggcacc ggtcaggcat ctgtcacttt aagttttaaa gcaggcaccg atccggacga
    11941 agccgtgcag caagtacaaa accagctgca atcagccatg cgaaagttac cgcaggcggt
    12001 gcaaaatcag ggcgtgacgg tgcgtaaaac cggcgatacc aacattctga ccattgcctt
    12061 cgtctctacc gatggctcga tggataaaca ggatattgct gattatgttg ccagtaatat
    12121 tcaggacccg ttaagccgcg tgaatggcgt cggggatatc gatgcctatg gttcgcaata
    12181 ttccatgcgt atctggctgg acccggcgaa actcaacagt ttccagatga cggctaaaga
    12241 tgtcactgat gccattgagt cacagaacgc gcagattgcg gttgggcaac ttggtggtac
    12301 accttccgtc gataagcagg cgctcaacgc caccattaac gcccagtcac tgctgcaaac
    12361 accagaacag ttccgcgata tcaccttgcg ggtgaatcag gacggctcag aggtaaggct
    12421 gggcgatgtc gccaccgtcg aaatgggggc ggagaaatac gattatctta gccgcttcaa
    12481 tggtaagcca gcctccgggt tgggggtaaa actggcctcc agcgcaaacg aaatggcgac
    12541 agcggagctg gtgctcaatc gtctcgacga gctggcgcag tatttcccgc acggactgga
    12601 atacaaggtg gcgtatgaaa ccacctcgtt tgttaaagcc tccattgaag acgtggtgaa
    12661 aacgctgctg gaagctatcg ctctggtttt cctcgttatg tatctgttcc tgcaaaactt
    12721 ccgcgccacg ctgataccca ctatcgccgt gccggtggtg ttgatgggaa ccttctccgt
    12781 actttacgcc ttcggttaca gcgtcaacac cttaaccatg ttcgcgatgg tgctggcgat
    12841 cggtctgctg gtggatgacg ccatcgtggt ggtggaaaac gtcgaacgta ttatgagtga
    12901 ggaaggactc actcctcgcg aagccacacg taaatcgatg gggcagatcc agggggcact
    12961 ggtcgggatt gcgatggtgc tttcggcggt atttgtacca atggccttct tcggcggcac
    13021 caccggtgcc atctatcgcc agttctctat taccattgtt gcggcgatgg tgctgtcagt
    13081 actggtagcg atgctcctca ctccggctct gtgtgccacg ctacttaagc cactgaaaaa
    13141 aggtgagcat cacgggcaaa aaggtttttt tgcctggttt aaccagatgt ttaaccgcaa
    13201 cgccgaacgc tacgaaaaag gggtggcgaa aattctccac cgtagcctgc gctggattgt
    13261 gatttatgtc ctgctgcttg gcggcatggt gttcctgttc ctgcgtttgc cgacgtcgtt
    13321 cttaccgctg gaagaccggg gcatgtttac tacctcggta caattgccca gcggttcaac
    13381 ccaacaacag accctgaaag tcgttgagca aatcgagaaa tattacttca cccatgaaaa
    13441 agacaacatc atgtcggtgt ttgccaccgt tggttctggc cctgggggta acgggcaaaa
    13501 cgtggcgaga atgtttatcc gcctgaaaga ctggagcgaa cgcgacagta agaccggcac
    13561 ctcgtttgcc attatcgagc gtgcaacgaa ggcgtttaac caaattaaag aagctcgcgt
    13621 tatcgccagc agcccaccag caattagcgg tcttggtagt tctgcaggtt ttgatatgga
    13681 gttgcaggac cacgctggag cgggtcacga tgcgctgatg gcagcacgta atcagttgct
    13741 ggcgctggcg gcggaaaacc cggagctaac ccgtgtgcgc cataacggcc tcgatgacag
    13801 tccgcagttg cagattgata tcgaccagcg taaagctcag gcgctgggcg ttgatatcga
    13861 cgatattaac gacacactgc aaaccgcctg gggttcgagc tatgtgaatg actttatgga
    13921 tcgcggtcgc gtgaagaaag tctatgtgca ggcagctgcg ccgtatcgca tgctgccaga
    13981 tgacatcaat ctctggtatg tccgaaataa agatggcggc atggtgccct tctctgcttt
    14041 cgcgacctca cgctgggaaa caggctcgcc gcgtctggaa cgctataacg gttattctgc
    14101 ggttgagatt gttggggaag ccgcaccggg ggtcagtacc ggtacggcga tggatattat
    14161 ggaatcgtta gtgaagcagc tgccaaacgg ctttggtctg gagtggacgg cgatgtcgta
    14221 tcaggagcgg ctttccggcg cgcaggctcc ggcgctgtac gccatttcct tgctggtggt
    14281 attcctgtgt ctggcggcat tgtatgaaag ctggtcggta ccgttctcgg taatgctggt
    14341 cgtgccgctg ggggtaatcg gcgcgctgct ggcaacctgg atgcgcgggc tggaaaacga
    14401 cgtttacttc caggtgggcc tgttaacggt cattggttta tcggcgaaaa acgccatcct
    14461 gatcgtcgag tttgctaacg agatgaacca aaaaggccat gacctgtttg aagcgacgct
    14521 ccacgcctgc cgtcagcgtt tacgcccgat tctgatgacc tcgctggcat ttatcttcgg
    14581 cgtattgcca atggcaacca gcacgggtgc cggttccggt ggtcagcatg cggtgggtac
    14641 tggcgtaatg ggcgggatga tttcagctac tattctggct atttacttcg tgccgctgtt
    14701 ctttgtgctg gtgcgccgcc gcttcccgct gaagccgcgc ccggaataag caataaaaaa
    14761 ggcgacatgc caatgtgtcg cctttttcaa ctttccgatt aagaacctgc tcagcgggtt
    14821 cttgctgttt atactttgtc tcaggaatta cttacgaagc ataacttcga taaagtcttt
    14881 ccagttcccc agttcacgtt caatcataac aacctctctt ataattatgg gtattctacg
    14941 gaaacaatat accgtggtga agctaattta ctcgattgct gcgatgacta cctccggggg
    15001 acaaacctta tgtaaatact atggtcctgc ggtgataatt tgtatgtgat acacagcaac
    15061 atttcgagat attcatacgg catctaatac tgatttaatt ctggttaaaa tacagacaga
    15121 taacaagatg aatattctta atgtttacgt taaaaatgtt taatattatt taatagttgt
    15181 taatttgaat acttcgataa tgttatattt tctgataatc atttgcaggc aaaatgtttt
    15241 cacccttaaa tgagtattta ttctcataaa tcgaaaaagg attcattatg gttacacttt
    15301 acggcatcaa aaattgtgac accattaaaa aggctcgccg ttggctggaa gccaataaca
    15361 tcgactatcg ttttcatgat taccgcgtcg atgggctgga cagcgaatta ttgaacggtt
    15421 ttatcaacga attaggctgg gaagcgttac tcaacacccg tggtacaacc tggcgtaaac
    15481 tggacgaaac tacccgcaat aaaatcaccg atgcggcctc tgcggcggca ttaatgactg
    15541 aaatgcctgc aattatcaaa cgtccattgc tctgcgcgcc tggtaagcct atgctgctgg
    15601 gtttcagtga ttccagttat cagcaatttt tccatgaggt gtagtctatg tcgtgcccgg
    15661 ttattgagct gacacaacag cttattcgcc gcccttccct gagtcctgat gacgcaggat
    15721 gtcaggcttt gttgattgaa cgtttgcagg cgatcggttt taccgttgaa cgcatggatt
    15781 ttgccgatac gcagaatttt tgggcatggc gtgggcaagg tgaaacgtta gcctttgccg
    15841 ggcataccga cgtggtgccg cctggcgacg ccgatcgttg gatcaatccg ccgtttgaac
    15901 ccaccattcg tgacggcatg ttattcgggc gcggtgcggc agatatgaaa ggctcgctgg
    15961 cggcgatggt ggtagcagca gaacgttttg tcgcacaaca tcccaaccat acggggcgac
    16021 tggcatttct gatcacttct gatgaagaag ccagtgccca caacggtacg gtaaaagtcg
    16081 tcgaggcgtt aatggcacgt aatgagcgtc tcgattactg cctggtcggc gaaccgtcga
    16141 gtatcgaagt ggtaggtgat gtggtgaaaa atggtcgtcg cggatcgtta acctgcaacc
    16201 tcaccattca tggcgttcag gggcatgttg cctacccaca tctggctgac aatccggtac
    16261 atcgcgcagc acctttcctt aatgaattag tggctattga gtgggatcag ggcaatgaat
    16321 tcttcccggc gaccagtatg cagattgcca atattcaggc gggaacgggc agtaacaatg
    16381 ttattccggg tgaactgttt gtgcagttta acttccgctt cagcaccgaa ctgactgatg
    16441 agatgatcaa agcgcaggta cttgccctgc ttgaaaaaca tcaactgcgc tatacggtgg
    16501 attggtggct ttccgggcag ccatttttga ccgcgcgcgg taaactggtg gatgcggtcg
    16561 ttaacgcggt tgagcactat aatgaaatta aaccgcagct actgaccaca ggcggaacgt
    16621 ccgacgggcg ctttattgct cgcatggggg cgcaggtggt ggaactcggg ccggtcaatg
    16681 ccactattca taaaattaat gaatgtgtga acgctgccga cctgcagcta cttgcccgta
    16741 tgtatcaacg tatcatggaa cagctcgtcg cgtgatgagt ggttctgcaa gaggaaataa
    16801 gcatggactg gctggctaaa tattggtgga ttctggtgat tgtctttttg gtaggcgtcc
    16861 tgctgaacgt gattaaagat ctcaagcgcg tcgaccataa gaaatttctc gccaacaagc
    16921 cggagcttcc cccgcatcgt gatttcaacg ataagtggga cgatgacgac gactggccga
    16981 aaaaggatca accgaagaag taagctgagt gctcaaccgt aggccggata aggcgttaac
    17041 gccgcatccg gcaatggtga acgatccctg atgcgacgct cacgcgtctt atcaggccag
    17101 gtaatctgtc ttttacatca tttcaatcac atcatcatcg ccaggcttac cgccacttag
    17161 cgcttggtcg aaataatgtt tcggaatggt ataacgcaag tgatccaggg caaattgcat
    17221 actgcgattg tcgatcgcat ggcctaaatc ctccacgata tccagcgtca cgtcaccacc
    17281 ggcactgatt aacgcttcct gcgccgcaac tgcatgcgcc agatcgataa ccggatcttc
    17341 accaccatga atcaagtgaa ttgtcgtggc tgtagacgcg gtctccggca ggctcgcata
    17401 gcgtccgtta aaggcaatga cgcgcgaagc aaggcccggt tcggctttaa tgctctccag
    17461 cgccataatc gcgccttgtg agaaaccaat tagcgccgtg gcgttagccc ctactccgct
    17521 ctgtttctgc cagtagcgta ccgtttcaat aaacgtcggc atgatggcat ccacacgcgc
    17581 ctggcgatta tcttccgtaa taccctgcac tgaaaaccac tgacgccccg ccggattacc
    17641 gctcggctcc gcgccaccga cactcaccac cagcgcatca ggaaacagcg gtgcaaacca
    17701 gctgcctatt tcccccattg ccaccgggtt atccccgaca ccgtggaaaa gcagtaacaa
    17761 ctgttgtgca ggtttatccg ggctttgaac aacaaaatgg tcatgtttca tggcgaactc
    17821 cttaactgat gtcattaatt ttacgccgct gactcacaat gaccatggga gaaaattgaa
    17881 ggagtcagtg aaaaaattgc cattgcgtta tgcgatcgcg cagacgctca gtgcgaacct
    17941 catttagtgc gaacagcgcc tgcgcggcct cttcccgctg gcgaaccagt aacatcttgc
    18001 ggcctgaaag tttaagtgtg gtacataact gcgcgtcact ggcgtttttc tgtaaacgcc
    18061 cacgcagcgc cggtaatgcc agttcactgg tttgtagcag acgcaataag caacctaacg
    18121 atgttaatag cggacgatga gcgaaagcaa aaccggccag ttcaagccag tcgtcgtcag
    18181 aaaggatggc atcgtttagt ggatcaacag gaagcatttc gccattccac tgcgcgagag
    18241 cttgcgcatc gcgacgtaaa cggtaatgct cacgttcagc cagctgttta cccgcatcac
    18301 tcatcggtaa cagcgccatc gccgtatagc aaccgctgct ggcttcccga tgattaccca
    18361 tccgcaccag cacaaaaccg cagcgttgcc agaaacgcca taactcccca gtgtaaccaa
    18421 aactcaccga aagatagtcg aggtcatgcg tatattgcaa agctccagca ataagttgtc
    18481 gccctgtgcc ttcccgctga cgagccggat gaactgctat ccggctaacc cgccttccgc
    18541 gcaatgtcgc cgccagtgga ttgctgccgt gcgccgccag cgactgggcc accagattac
    18601 cccgcgggcg acgaaaacct gcccataccg cctgactgag ttcttgagat aatccgccct
    18661 catccaccag ccacaacgcc ccggcaatct cgttttcgcc agccgcctgt aaaaaatgtt
    18721 gccctggtgc atccatcatc cggcgtaaat ccagcggcga ggtccggtag tgcgcaccag
    18781 acaataactg ataaaccttt aacggcgttt ctggctcgct tcgccataac gtttgttcaa
    18841 atgcggaaat gacgatattg ccttgtggtg tatgggtgaa gttttcatcg ttaaaaacca
    18901 gtgcctcact aaccattttt tccagcgggc atccctgtgc ccagcggatc ggctgttgca
    18961 gttcaaaacg gtgtaaatgc ggaaagcgag cgcaaaattt cagcaaaaaa ccacgtccgg
    19021 taccttcgta gccctgcacc gtagtggtta acaacgttcg aggaaaacgc gataccagtt
    19081 gatgcaacaa cggcgcaggt atggctgcgg cttcgtcgac caccagccag tcggcttgct
    19141 catcgctggc taacaaggca tccggtgcaa taaagcgaaa cttctcgccc gcaaattgtg
    19201 ccagtacatc cgttgccgct tttgcgggcg cggtgacaat cgcactgccc gcaatacgag
    19261 aaatgagttg ccctgccaac gccgacttac cgcgcccacg cgcagccgtt accgctgcca
    19321 cgcccggcgg catggtcagt agctgctgta agagttgctg ttgttctggt tgtggtgcgc
    19381 cggtcgcggg gtgccagtca gtacggggag taaaatgcgc caacgagaac ggctggtttt
    19441 gccgccagag gatagcgtcg ttatccgccg taagtacgcg tttgagatgc tggacaaaat
    19501 gcggcgtcgc aatagggtca gggcaatcac tccagcgcag cgagtcggca tcaggttggt
    19561 tttcccactc ttcccataca gggagtaaca aaaccagcca gcttcccgct ttcaacgttc
    19621 cgctaagtgc tgcaaaggca gcggcatcaa agccgtggcg ggcgtcgaat accgcatgcc
    19681 ggaactcgcg cccaagtaaa gtttgtagcg ccgagggtga acagtggttt tcagcatcta
    19741 gctgcggcga aatccacagc cagtcgccag gtaaggcatc acgcaactta agagcatgat
    19801 caaaacacca accctcttcc ccgctcaaca ccagcaagcg gcagatccct tcacgtttca
    19861 tttgcgctgt taatgtgtaa agcgcagtca gttcagccat ccctgccccg gaaattaaat
    19921 gcttttacca aaagtattgc attgtgccgg atcgccgctg tcgaaaccac gtttaaacca
    19981 gctgtagcgt tgctgagaag tgccatgtgt gaaactgtct ggtactactc gcccctgact
    20041 ttgctgttgt aaacggtcat cgccgatggc ctgcgccgca ttcagcgcct cttccagatc
    20101 gccagtttcc agaacgcctt gctgctgcat actatgcccc cagacaccgg caaaacagtc
    20161 ggcctggagt tccatacgca cagataagcg gtttacttcc gcctgcgtcg cgttttgttg
    20221 cagttgacga actttcggct cgatgcctaa cagtttctgc acatgatgac cgacttcatg
    20281 ggcgataacg tacccctggg caaaatcgcc atccgcgcca agtttgtctt tcatgtcatc
    20341 atagaaggag agatcgatat aaactgtgcc atccgccggg caatagaacg gccccataat
    20401 ggactggccc gccccgcagc cggtgcgcgt cattccacgg tacatgacca gcttcggttg
    20461 ctgataggtc ttacccatct tttcgaactg ttgtccccag gtgtcttccg tggttgcctg
    20521 aatcaccgag gtgaattttg cggcttcgtc ttcattaggg ctaattgacc gcgttgattg
    20581 ttgttgagaa accggctgcc cggtcatcaa cccggttaaa tcaacgccat agtagcctgc
    20641 aaccagcacg actatcagta aaatcagccc gcctttaccg ctcggcagac ggaaaccggg
    20701 accgcccatt gacggaccgc cagagctgtt gcgcctgtct tcaacattgt cactttcacg
    20761 tcgcccttgc caacgcatag atacctcaac aatatattca ttatagaaat gatcgtaggt
    20821 ggttcagagg aagattacca caggaaagcg ggatgaaatg gcggaggaat gagcgttcaa
    20881 tgcatatatt ccgagagcgg ttgtcttgcc gtgccagctg cacggcaaga tgatgaacag
    20941 aaaaatcagt ccagctgtac acccaggcgg cgggcgacgg cttcataggc ttcgatcagg
    21001 ccaccgaggc tctggcggaa acggtctttg tccattttct ccagtgtttc tttgtcccac
    21061 aggcggctgc cgtccgggga gaactcatcg cccagtacca cttcaccttt gtacagacca
    21121 aactccagct tgaagtcgac cagaatcaga ccagcatcat cgaacagttt tttcagcacg
    21181 tcgttcgctt tgtaggtcag ctctttcata cgcgccaggt tctctttgct cacccagcca
    21241 aaggtttcgc agtaagattc gttgaccatc ggatcgtgca tggcatcgtt tttcaggaac
    21301 agatcgaaca gcggcgggtt cagctcaata ccttcttcga ttccaagacg tttcactaga
    21361 gagccagcag cacggttacg cacgacacac tcaaccggca ccatatccag ctttttcacc
    21421 agacattcgg tatcggagag cagacgctcc atttgagtcg ggatacccgc ttcagccagt
    21481 ttgctcataa tgaagtagtt gaacttgttg ttcaccatac ctttgcgatc aaactgctca
    21541 atgcgcgcgc catcccctgc tgacgtatca ttgcggaatt cgagcaccaa caggtccggg
    21601 ttttccgtgc tgtatacggt tttcgcttta ccacgataca actcagcttg cttttgcatc
    21661 tttatcactc ctgggtgtga attaacgttt taaaatcttt tgctgtctgg tgtgccggat
    21721 gttttgtcgg atgcggcgtg aacgccttat ccgaactacg tccgacaaac aaatttcgtg
    21781 cgaattaccg ctaaaatcgc gttttcctgc cgacgcacac gtttgcgtat catatcagaa
    21841 aaaagggccg gatgattcca gccctgtatt tttacttgct aaacgcagcc tggaagacag
    21901 ctaccagcgc gtcgttctga ctctgagtca gagtatgacc tttcggatcg atgaactgta
    21961 ggctgctgcg gttatctaaa tcgccaacct gcagtttata gtcaccggat gccaggcctg
    22021 gatcgctcgc gcccagttcc tgccagtcgc tgtcagacag cggcttataa gttacggcca
    22081 tgttgccctg cgaacgggtg ctgtcggtca ctttcatgcc cactttttcc agcgccgctg
    22141 gcagacgttg ccaaaccaca ttgaacggcc cgcgtacgac cagcattggt aaaccggtgt
    22201 catcagctgc actttgtacg tccatagtgg tggaggcacg attttgcgca gcgttcgcgg
    22261 cgtcagtggc agatttatcc agaccggcgg aaataacgtt catcatctcc gtgctgtaac
    22321 gctgcatgga agccgcgtct gcaaccggtt tgcccgcctg ttccaggttc agcagtttaa
    22381 ccgtaaccgc ctgctgataa ccctgcggct taacagagat ttgataacga ccacgatact
    22441 gctcgtcttc gtccagacgg ttccattgta cccaatcggt ggtcagtgtc tgaccagcat
    22501 catcacgttg ggtgatggtg tagtttttcg cctgcagcac gctaaccacc tgcggccaca
    22561 gagtattgcc acgaccattt tccaccagca atgaagcggt atcgcccgtg aactgggtac
    22621 gcgcgccaga aaccagtgcc aacggctggg ctggtggacg aatgtccagc gccttaccga
    22681 cagcaccact accgttggtc accgggattg cataatcacc ggaggtcacc ggcaaaatca
    22741 ttccagccgg ggcatgaagc tccgcaagcg gtgccgcttc caggtaggct tcatcaccac
    22801 tgacctgacg cttatagcgt gagtcagaac tacaggcagc gagtaataaa acaagcgaaa
    22861 cacccgcaac ctttgccagg cgcgactttt gaacagagta agccatcaaa tctccctaaa
    22921 ctttacagca aaccggcatg cttaagcgcc gctctgaccg tctcacgacc actgtcggtg
    22981 attggtgtca ttggcaggcg cagcgtatcg gtcgccacaa gacccagttc cttacatgcc
    23041 catttcaccg ggattggatt gggttcgaca aatagtttgt tgtgtaatgg catcagacgc
    23101 tgattaataa cgcgtgcctc ggcaaaatgc ccttctgctg ccagtttgca catctgggcc
    23161 atatcacgcg ctgcgacgtt agccgtaacg gaaataactc catgaccgcc caattgcatg
    23221 aagtccagcg cgctcgcatc atcgccgctc agcagaacaa aatcatctga aaccagctct
    23281 ttgatctggt ttacacgcgt taagttccct gttgcctctt tgattccgat aatatttttt
    23341 actttcgcca gacggcccac cgtttccggg agcagatcgc agccagtacg ggacggcaca
    23401 ttatacagaa tttgcggcag gtcagtatgc tcagcgatgg ctttgaaatg ctgatacaaa
    23461 ccttcttgcg acggacgatt gtagtaaggg gttaccgtca ggcagccgac gataccactg
    23521 tcattgaagc gctgcgtcag gctaatggct tccgcagtag cgttagcgcc ggtcccggca
    23581 attaccggaa tgcgcccatc agccagatcc agcgtcatca tcaccacatc agcatgttcg
    23641 tcatgattta aggtagcgga ctcgccagtg gtgccaacag aaacgatcgc cgaagtaccg
    23701 ctggcgacat gataatcaat cagttttttc aagctagccc gacagacatt acctttttca
    23761 tccatcggag taacaatcgc gacaatactt cccgtgaaca tgggccatcc tctgtgcaaa
    23821 caagtgtctc aatggtacgt ttggtatggc attaaaagca agcagacaga accgttctga
    23881 ttgttgtatg catgattttt ttatgctttc cttaagaaca actcacccct taaaggaata
    23941 accagtttga cactgtcatc gcaacattat ctggtgatca ctgcgttggg tgccgatcgc
    24001 cctggaattg tgaacaccat cacccgtcat gtcagtagtt gcggctgtaa tattgaagac
    24061 agtcgcctgg cgatgctggg agaagagttc acgtttatca tgctgctttc cggttcatgg
    24121 aatgccatca ctctgattga atcaacgtta ccgttgaaag gtgccgaact ggatctttta
    24181 atcgtgatga agcgcacgac ggcgcgtccg cgtccgccaa tgccagcatc tgtctgggtt
    24241 caggtcgatg ttgcagactc cccgcattta attgaacgct tcacagcact tttcgacgcg
    24301 catcatatga acattgcgga gctggtgtcg cgcacacaac ctgctgaaaa tgaacgggct
    24361 gcgcagttgc atattcagat aaccgcccac agccccgcat ctgcggacgc agcaaatatt
    24421 gagcaagcgt tcaaagccct atgtacagaa ctcaatgcac aaggcagtat taacgtcgtc
    24481 aattattccc aacatgatga acaggatgga gttaagtaat gaatccactg aaagccggtg
    24541 atatcgcacc gaaatttagc ttgccggatc aagacggaga acaagttaat ttgaccgact
    24601 tccagggaca gcgtgttctg gtttatttct acccgaaagc catgaccccc ggctgtaccg
    24661 tacaggcctg cggcttacgc gataacatgg atgagttgaa aaaagcgggc gttgatgtgc
    24721 tgggtatcag caccgataaa cccgaaaaac tctcccgttt tgcggaaaaa gagctgctta
    24781 actttacgct cctgtctgat gaggaccacc aggtgtgcga acaattcggc gtctggggtg
    24841 aaaagtcctt catgggcaaa acctacgatg gtattcatcg catcagcttc ctgattgacg
    24901 ctgatggcaa aatcgaacat gtctttgacg atttcaaaac cagcaatcac cacgacgttg
    24961 tgctgaactg gctgaaagaa cacgcctgat tactttgctc cattccgtgc tggctgcgct
    25021 tgcggccagc atacctcact tctcgtgatc aagatcacat tctcgctttc ccctgcgaca
    25081 cgggtgtcga atccattttt tgctgaacgt taatgaccat catttttgta ccgttcagaa
    25141 tccagttaat acataactta ttgaatagat tgagttaatc agaatggcat cctttataca
    25201 atatgaaatg caatgtttca tatcattttc aaggagccga catgaaccgc tttgtggtgg
    25261 ccgaaccact gtggtgtaca ggatgtaata cctgtctcgc tgcctgttcg gacgtgcata
    25321 aaacgcaagg tttacagcaa cacccgcgcc tggccctggc gaagacgtca acaatcactg
    25381 cccctgtcgt gtgtcatcac tgtgaggaag ccccttgcct gcaggtctgc ccggtcaatg
    25441 ccatctctca gagggatgat gcgatccaac tcaacgaaag cctctgtatt ggctgcaagc
    25501 tttgcgccgt ggtctgccca tttggcgcaa tcagcgcttc aggaagccgt ccggtgaatg
    25561 cccatgcgca atatgttttt caggctgaag gctcactcaa agacggcgaa gaaaacgtgc
    25621 cgccccaaca tgcgttgctg cgctgggaac cgggtgtcca gaccgtcgcg gtgaaatgcg
    25681 acctgtgtga tttcttgcca gaaggtccgg cctgcgttcg cgcttgcccg aatcaggcgt
    25741 tacggctgat caccgacgat agcctgcaac gtcagatgaa agaaaaacag cgccttgccg
    25801 caagctggtt tgccaatggc ggggagtctc ccctttccct cactcaggag caacactaat
    25861 ggatgccctg caattattaa cctggtcgct gatcctctat ctgtttgcca gtctggcttc
    25921 gctgttttta ctcggtctgg acagactggc tattaagctt tccggcatca catcgctggt
    25981 gggcggcgtg attggcatca tcagcggaat tacgcaatta catgctggtg taactttagt
    26041 cgcccatttt gccacccctt ttgactttgc cgatttaacc ctgcgaatgg atagcctctc
    26101 ggcatttatg gtgctagtta tctccttgct ggtggtggtt tgttcgctct attcattgac
    26161 ttatatgcgc gaatacgagg gcaaaggcgc ggcggcgatg ggcttcttta tgaatctttt
    26221 catcgcatcg atggttgccc tgctggtgat ggacaacgct ttttggttca tcgtgctgtt
    26281 tgaaatgatg tcgctgtctt cctggtttct ggtcattgcc aggcaggata aaacgtcgat
    26341 caacgctggc atgctctact tttttatcgc ccacgccgga tcggtgctga ttatgatcgc
    26401 cttcttgctg atggggcgcg aaagcggcag cctcgatttt gccagtttcg cacgctttca
    26461 ctttctccgg ggctggcgtc ggcggtgttt ctgctggcct ttttcggttt tggcgcgaaa
    26521 gccgggatga tgccgttgca cagctggttg ccgcgcgctc accctgccgc accatcgcac
    26581 gcttcggcgt tgatgtctgg cgtaatggtc aaaatcggta ttttcggcat cctgaaagta
    26641 gcgatggatc tgctggcgca aacgggtttg cctctgtggt ggggcattct ggtgatggcg
    26701 atcggcgcaa tctccgcgct cctgggcgtg ctatatgcgc tggcggaaca ggatatcaaa
    26761 cggctgctgg cctggagtac cgtcgaaaac gtcggcatta ttttgctggc agtcggtgtg
    26821 gcgatggacg gtctgtcact gcacgacccg ctgctcaccg tggttggact gctcggcgca
    26881 ctgtttcatc tgctcaacca tgcgctgttc aaagggctgc tatttctcgg cgcggacgcg
    26941 attatttcgc gtttgcatac ccacgacatg gaaaaaatgg gggcactggc gaaacggatg
    27001 ccgtggacag ccgcagcatg cctgattggt tgcctcgcga tatcagccat tcctccgctg
    27061 aatggtttta tcagcgaatg gtacacctgg cagtcgctgt tctcactaag tcgtgtggaa
    27121 gccgtagcgc tacaacttgc gggtcctatt gctatggtaa tgctggcagt cactggtggg
    27181 ctggcagtaa tgtgcttcgt caaaatgtac ggtattactt tctgtggtgc gccgcgcagt
    27241 acacacgctg aagaggcaca ggaagtgcca aatacgatga tcgtcgccat gctactgctc
    27301 gcggcactct gcgtattaat tgcgcttagt gccagttggc tggcaccgaa gataatgcat
    27361 attgcccatg cgtttaccaa tacccctccc gtcactgtcg ccagcggaat agcacttgta
    27421 cccggcacgt ttcatacacg ggtcactcct tcattactgt tgctgttact actggcgatg
    27481 cctttgctgc caggccttta ctggctgtgg tgtcgttcgc gccgcgcagc gtttcgtcgc
    27541 acaggagatg cctgggcatg cggctacggc tgggaaaacg cgatggctcc gtcaggcaat
    27601 ggcgtgatgc agccgctgcg tgtggtcttc tctgcgttat ttcgtctacg acaacagctc
    27661 gaccctacgc tgaggctaaa taaaggtctt gcgcacgtca ccgccagggc tcagagcaca
    27721 gaacccttct gggatgagcg ggtgatccgc cccatcgtga gcgccaccca acggctggtc
    27781 aaagaaatac agcatctgca aagcggcgac tttcgtctct attgcctgta tgtggtcgcc
    27841 gcactggttg tgctgctaat cgctattgcc gtctaaggaa atcaccatga gacaaactct
    27901 ttgcgacgga tatctggtca tttttgcgtt agcacaggcc gtgattctgc tgatgctaac
    27961 cccacttttt acgggtattt cccggcagat acgcgcgcgt atgcactccc gccgcgggcc
    28021 ggggatctgg cagaattatc gcgatatcca caaactgttt aaacgccagg aagttgcgcc
    28081 gacatcttca ggtctgatgt tccgcctgat gccgtgggta ttaatcagca gcatgctggt
    28141 gctggcgatg gccttaccac tatttattac cgtttcccct tttgcgggcg gcggcgatct
    28201 gatcaccctt atctatcttc ttgccctgtt tcgttttttc tttgctcttt ccgggctgga
    28261 taccggaagt ccgtttgcgg gagtcggtgc cagtcgcgag ttgacgctcg gcattctggt
    28321 cgaaccaatg cttattctct cactgctggt attggcgctg atagcaggtt ccacgcatat
    28381 cgagatgatc agcaatacgc tggctatggg ctggaactcg ccgctaacca ccgtactggc
    28441 gttactggcc tgtggttttg cctgcttcat tgagatggga aaaattccct ttgatgttgc
    28501 tgaagcagaa caggaattac aggaaggccc gctgaccgaa tattccggtg ccgggctggc
    28561 gctagcgaaa tgggggctgg ggctgaaaca ggtcgtgatg gcagcactgt ttgtggccct
    28621 gtttctgccc tttgggcgcg cgcaagaact ttctctcgcc tgcctgctga cttcacttgt
    28681 cgttacgctg ctcaaggttt tgctgatttt tgtactggcc tcaatcgcag aaaacacgct
    28741 ggcacgcggg cgttttttac tcattcacca tgtgacctgg cttggcttca gccttgctgc
    28801 gcttgcatgg gtcttttggt taaccggtct gtaaggagca ctgacggaat atggaaaatc
    28861 ttgctctgac gacgttaatg ctgcctttta tcggcgcact ggtcgtttcg ttttcgccac
    28921 aacgtcgggc cgccgaatgg ggggttttgt tcgccgcgct gaccacgctg tgcatgttgt
    28981 cgctgatctc cgcgttttat caggccgata aagttgccgt cacgttgacg ttggtcaacg
    29041 tgggggatgt ggcattattt ggcctggtca ttgatcgcgt gagtacgctg attctgtttg
    29101 tggtggtgtt tctcggtttg ctggtcacga tctactccac gggttatctg acggataaaa
    29161 atcgcgaaca cccgcataac ggcacgaatc gttattacgc atttttgctg gtgtttatcg
    29221 gcgcgatggc gggactggta ctctcctcga cgctgctcgg tcagttgttg ttttttgaaa
    29281 ttacgggcgg ctgctcctgg gcgttgatca gttattacca gagcgataaa gcgcagcgtt
    29341 cagcactaaa agcgttgctt atcacccata tcggttcgtt ggggttgtat cttgccgccg
    29401 ccacgctgtt tttgcagacc ggaacgtttg tgcttagcgc gatgagcgag ttacatggcg
    29461 acgcacgtta tctggtttat ggcggcatcc tgtttgccgc gtgggggaaa tcggcccagc
    29521 taccgatgca agcgtggcta ccggacgcaa tggaagcgcc aacaccgatc agcgcctatc
    29581 tccacgccgc atcgatggtg aaagtgggcg tttacatttt tgcccgcgcc attatcgacg
    29641 gcggcaatat cccgcatgtg attggcggcg ttggcatggt catgacactg gtcaccattc
    29701 tttatggctt tctgatgtat ttgccacagc aggatatgaa gcggttgctg gcctggtcga
    29761 ccatcactca acttggctgg atgttcttcg gcttgtcgct ctccatcttc ggctcgcggc
    29821 tggcgctgga gggtagcatc gcctacatcg tcaaccacgc gttcgctaaa agcctgtttt
    29881 tccttgtagc aggtgcgctg agttacagct gcggcacgcg cttgttgccg cgtctgcgtg
    29941 gcgtattgca caccctgccg ttgccaggcg tggatttctg cgtggcagcg ctggcaatta
    30001 ccggtgtgcc gccgttcaac ggcttcttca gtaaattccc gctgtttgcc gccggttttg
    30061 cgttgtcagt ggagtactgg atcctgctgc ccgccatgat tctgctgatg attgaatcgg
    30121 tcgccagttt cgcctggttt attcgctggt ttggtcgcgt cgtgccgggc aaaccgagcg
    30181 aggccgtcgc cgatgccgca ccgctgccag ggtcaatgcg cctggtgttg attgtactga
    30241 ttgtgatgtc gctgatttcc agcgtaatcg ccgcgacctg gttgcagtaa ggagatgatg
    30301 aatgaccggt tctatgatcg taaataatct ggcgggactg atgatgctga catcgctgtt
    30361 tgtgattagc gtcaaaagct atcgcctgtc atgcggattt tacgcctgcc agtcgctggt
    30421 gctggtgtct attttcgcca ctctctcgtg cctgttcgcc gcagagcaac tgctgatctg
    30481 gtccgccagc gcctttatca ccaaagtgct gctggtaccg ttaatcatga cttacgctgc
    30541 acgaaatatt ccccagaaca tcccggaaaa agcgttattc ggtccggcaa tgatggcact
    30601 gctcgcggcg ttaattgtcc tgctttgcgc atttgtcgtt cagcccgtga agctaccgat
    30661 ggctaccggg ctgaaaccgg cgctggcggt agcgttaggt cattttctgc ttggcctgct
    30721 gtgcattgtc agccaacgca atatcctgcg gcaaattttt ggttactgcc tgatggaaaa
    30781 cggctcccat ctggtgctgg cgcttcttgc ctggcgagca ccggaactgg tggaaatagg
    30841 tatcgctacc gacgccatct tcgccgtcat tgtgatggtg ttactggcaa gaaaaatatg
    30901 gcgtacccac ggcacgctgg acgtgaacaa cttgaccgcg ctgaagggat aatgagatga
    30961 gttattctgt gatgttcgct ttactcctgc tcacgccgct gcttttttcg ctgctctgtt
    31021 ttgcctgccg gaaacggggg ctctctgcga cttgtacggt gactgtatta catagcttag
    31081 ggatcacact gctgctgatt ctagcactct gggtggtcca aactgccgct gatgcaggag
    31141 aaatattcgc tgcgggactg tggcttcata ttgatggtct gggcggtttg ttcctcgcca
    31201 ttcttggtgt gattggcttt ctcaccggtg tttactcgat tggctacatg cgtcatgaag
    31261 tggcacacgg cgagctttca cccgttacac tgtgcgatta ctacggtttc ttccatctgt
    31321 ttttgttcac catgctgctg gttgttacca gcaataacct gattgtgatg tgggcggcga
    31381 tcgaagccac caccttaagc tcggcgtttc tggtaggcat ttacggtcag cgttcatcgc
    31441 tggaatcagc atggaagtac atcattattt gtactgttgg tgtcgctttt ggtctgttcg
    31501 gtaccgtgct ggtatacgcc aacgccgcca gcgttatgcc gcaggcagaa atggcgatat
    31561 tctggagcga ggttcttaag caatcgtcct tgcttgaccc aacattaatg ctgttggcct
    31621 ttgtgtttgt gctaattggc tttggtacca aaaccgggct atttcccatg cacgcctggc
    31681 tgccggatgc tcacagtgaa gcgccgagtc cggtcagcgc cctgctctcc gccgtattgc
    31741 tgaactgcgc gctgttggtg ctgattcgct attacatcat tatttgccag gccatcggca
    31801 gcgatttccc caaccggttg ttgctcatct tcggcatgtt gtcggttgcc gtggcggcat
    31861 ttttcattct ggtacagcgg gacattaagc gtctgctggc gtactccagc gtggagaaca
    31921 tggggctggt cgcggtggcg ctaggcattg gcgggccgct gggaattttt gccgcgctgc
    31981 tgcacacctt aaaccacagt ctggcaaaaa cgctgctgtt ctgcggttcc ggcaatgtac
    32041 tgctcaagta cggcacgcgc gatctcaacg tcgtctgtgg gatgctcaaa atcatgccat
    32101 ttaccgccgt gctgtttggt ggcggtgcgc tggcgctggc gctggcaggg atgccgccct
    32161 tcaacatttt tcttagcgaa tttatgaccg ttaccgcagg actggcacgt aatcacctgc
    32221 tgattatcgt cctgctgtta ttgctgttaa cgctggtgct ggcgggcctg gtacggatgg
    32281 ctgcgcgggt gttaatggcg aaaccgccgc aggccgttaa ccggggtgat ctcggctggt
    32341 tgaccacctc gccaatggtg attctgctgg tcatgatgct ggcgatggga acgcatattc
    32401 cacaacctgt catcaggatc ctggcgggcg cttccactat agtcctctca gggacgcacg
    32461 acctgcctgc acaacgtagc acctggcatg attttttgcc ttcaggcacc gcatctgttt
    32521 cggagaaaca cagtgaacgt taattcatcg tcaaatcgtg gcgaagcgat tctcgccgcc
    32581 ctgaaaacgc agttccccgg cgcggtgctg gatgaagagc gacaaacgcc tgaacaggtc
    32641 accattacgg tgaaaatcaa tctgctgcct gacgttgtac attatcttta ttatcaacat
    32701 gatggctggc ttccagtcct gtttggcaac gacgagcgga cacttaacgg tcattacgcg
    32761 gtttattatg ccctttcaat ggaaggggcc gaaaaatgct ggatcgtggt gaaggcactg
    32821 gtcgatgccg acagtcggga gtttccgtca gtcacaccgc gcgtccctgc cgcggtctgg
    32881 ggcgagcgag aaattcgcga tatgtacggg ctgattccgg ttggcctgcc ggatcagcgt
    32941 cgcctggtgt tgcccgatga ctggccggaa gatatgcatc cgctgcgcaa agatgcgatg
    33001 gattatcgac tgcgccctga accgacgaca gattccgaaa cgtatgcgtt tatcaatgag
    33061 ggcaacagcg atgcgcgggt gatccctgtc ggcccgctgc atatcacctc ctatgaaccg
    33121 ggtcacttcc gcttgtttgt ggatggcgag caaattgtcg atgctgatta ccgcctgttt
    33181 tatgtccatc gcggcatgga gaaactggca gaaacgcgga tgggctacaa cgaagtgacc
    33241 ttcttatcgg accgcgtgtg tgggatttgc ggttttgccc acagtgtggc ctataccaac
    33301 tcggttgaaa atgcactggg gattgaggtg ccgcaacgag cacatactat tcgctcgatt
    33361 ctgctggaag tcgagcggct acacagtcat ttgctcaacc ttggcctctc ctgccatttt
    33421 gttggttttg ataccggctt tatgcaattt ttccgcgtgc gggaaaagtc gatgacgatg
    33481 gcggaattgc tgaccgggtc gcgtaaaacc tacggtctga atctgattgg tggtgttcgc
    33541 cgcgatattc tcaaagagca acgtctgcaa acgctgaaac tggtgcgcga gatgcgcgcc
    33601 gacgtgtcgg agctggtaga gatgctgctt gctacgccga atatggaaca acgcactcag
    33661 ggcattggca ttctcgaccg acaaatcgcc cgtgattata gccctgtagg gccgctgatc
    33721 cgcgcagtgg ttttgcccgt gatttgcgct ttgatcaccc ctacgccgac tacggcaata
    33781 ttccaaaaac actgtttacc tttaccggcg gcgatgtttt ctcccgcgtg atggtccgtg
    33841 tcaaagagac gtttgattcg ctggcaatgc tggaatttgc cctcgacaac atgccggata
    33901 ccccactgct gaccgaaggc tttagctata aacctcacgc attcgcgctg ggctttgttg
    33961 aagcgccacg cggtgaagac gtgcactgga gcatgctcgg tgataaccaa aaattgttcc
    34021 gctggcgctg tcgtgccgcc acctacgcca actggccggt gttgcgttac atgctgcgcg
    34081 gcaataccgt ttctgacgca ccgctgatta tcggtagcct tgatccctgc tactcctgta
    34141 ccgaccgtgt gacgctggta gatgtgcgca agcgccagtc aaaaaccgtg ctgtataaag
    34201 agatcgaacg ctacggcatt gatcgtaacc gttcgccgct gaagtaagga cagaagatgc
    34261 tgaagttact gaaaactatt atgcgcgccg gaaccgcgac ggtgaaatat cccttcgcgc
    34321 cactggaggt cagcccaggc tttcgcggga aaccggacct gatgcccagc caatgtattg
    34381 cctgcggtgc ctgcgcctgt gcttgtccgg caaatgcgct gactatccag accgacgacc
    34441 agcaaaattc gcgcacctgg cagctctatc tggggcgttg tatttactgc ggacgttgtg
    34501 aagaagtgtg cccgaccaga gccatccagc ttaccaataa ctttgaactg accgtcaccc
    34561 ataaagccga tctctatacc cacgcgacgt tccatctaca acgttgcagc cgttgcgaac
    34621 gcccgtttgc cccgcaaaaa accgtcgcac tggctgctga attgttggca cagcaacaaa
    34681 atgctccaca aaaccgcgaa atgttatggg cgcaagcgag cgtctgcccg gagtgcaaac
    34741 aacgcgcaac gctgatcaac gacgatacag atgtaccgct ggtggctaag gagcagctat
    34801 gagtccagtg cttacgcaat atgtcagcca gcccatcacg ctggacgagc aaacgcaaaa
    34861 gatgaagcgg catttgctac aggatatccg tcgctcggct tacgtttatc gcatcgattg
    34921 cggcggctgc aacgcctgtg aaatcgaaat ttttgctgcc atcacccccg tattcgacgc
    34981 agaacgtttt ggcattaagg ttgtttcatc accgcgtcac gccgatattt tgttatttac
    35041 tggcgcagtc acccgggcga tgcgtatgcc tgcacttcgg gcgtatgagt ctgcccccga
    35101 tcataaaatc tgtgtttcct acggcgcgtg cggtgtcggc ggcggtattt tccacgatct
    35161 ctacagcgtc tggggcggca gcgacaccat tgtccccatt gatgtttgga tccccggctg
    35221 cccgccaaca ccggccgcca ccattcacgg tttcgccgtg gcgctcggtt tgctgcaaca
    35281 gaagattcac gcggtggatt atcgcgatcc caccggggtg actatgcaac cgttgtggcc
    35341 gcagatcccg ccatcacagc gtatcgccat tgagcgagaa gcgcggcggc tggcgggcta
    35401 tcgtcagggg cgagaaattt gcgatcggct cctgcgccat ttaagcgacg atcctacagg
    35461 aaatcgggtt aacacctggt tgcgcgatgc cgacgatcca cgtctcaata gtatcgttca
    35521 gcaactcttt cgcgtactcc gggggttaca tgactgaaga gtgcggggaa attgttttct
    35581 ggacgctgcg aaaaaagttt gtcgccagta gcgacgagat gccggaacac agctctcagg
    35641 taatgtatta ctcgctggct atcggtcatc acgttggcgt gattgattgt ctgaatgtcg
    35701 ccttccgctg cccactgacg gaatacgaag attggcttgc actggtcgaa gaggagcaag
    35761 cccgacgtaa gatgctgggg gtgatgactt ttggtgagat tgttattgac gccagccaca
    35821 ccgccctgtt gacccgggca ttcgcgccac tggcagatga cgcgacgtct gtgtggcaag
    35881 cgcgtagcat tcaattcatt catctgttgg atgaaattgt gctggaaccg gctatctatc
    35941 tgatggccag aaaaattgcg tgagaaggat ttctcattaa taaggactgt tgatggctat
    36001 gtcagacgaa gcgatgtttg ccccgccgca aggaataaca attgaagcgg taaacggaat
    36061 gctcgcggag cggttagcgc agaaacacgg caaggcgtct ttattacgcg ccttcatccc
    36121 gctgccgccg ccgttcagcc cggtacaact tattgaactg catgttctca aaagcaactt
    36181 ctattaccgc taccatgatg atggcagcga tgtgacggca acaacagagt atcagggcga
    36241 gatggtcgat tattcgcgtt acgccgtcct tctcggcagt agtggaatgg cggagctacg
    36301 ctttattcgt acccacggca gccgttttac tccccaggat tgcacactgt ttaactggct
    36361 ggcacgcatt atcaccccgg ttctgcaatc atggctcaat gatgaagcac agcaggtggc
    36421 gctgcgtttg ctggagaaag atcgcgatca tcatcgggta ctggtggata tcactaatgc
    36481 agtgctgtca catcttgatc tcgacgatct gatcgctgac gtcgctcgtg agatccatca
    36541 ttttttcggt ctggcttcag tcagtatggt actgggcgat catcgaaaga acgagaagtt
    36601 cagcctgtgg tgcagcgatc tttctgcctc acattgtgcg tgtctgccac acaatatgcc
    36661 tggcgacagt gtattgctga cacaaacgct acaaacccga caaccgacct tgacgcaccg
    36721 tgcagatgat ctgtttctct ggcaacgcga cccgttatta ctcttacttg catctaacgg
    36781 ctgcgaatct gcgctcctta taccgcttac ctttggcaac catacaccgg gtgcattgtt
    36841 gctggcgcat acctcttcca ctctctttag tgaggaaaac tgccagctac tacaacacat
    36901 agccgatcgc atcgctattg ccgttggcaa tgccgatgcc tgccgtagaa tgaccgattt
    36961 gcaggaaagt ttgcagcaag aaaaccacca gcttagcgag cagctccttt cgaatctggg
    37021 cgtcggtgac attatctatc aaagccaggc aatggaagac ctgctccagc aggtagatat
    37081 tgtggcgaag agcgacagta cggtgttgat ttgcggtgaa accggaaccg gcaaagaggt
    37141 gatcgccaga gcgatccatc aacttagccc gcgacacgac aagccgctgg tcaaaatcaa
    37201 ctgcgctgcc atccccgcca gtcttctgga aagtgagtta ttcggtcatg acaaaggggc
    37261 gtttactggt gcgattaata cccatcgtgg tcgttttgaa attgccgatg gcggcacgtt
    37321 gtttctcgat gaaattggcg atctgccgtt agaacttcag cctaaactgc tgcgcgtatt
    37381 gcaggaacgg gagattgagc gtctcggcgg gagtagaacg atcccggtaa atgtcagagt
    37441 cattgccgcc accaaccgtg atttgtggca aatggttgaa gatcgccagt ttcgcagcga
    37501 tctcttttat cgcctgaatg tcttcccact ggaattgcct ccgctgcgcg accgtccgga
    37561 agatatccct cttttagcaa agcatttcac gcaaaaaatg gcgcgccata tgaatcgttc
    37621 aattgacgcc atcccgaccg aggcgctacg ccagttgatg tcgtgggact ggccgggcaa
    37681 cgtgcgcgag ctggaaaacg tgattgagcg agcggtactg ctgactcgcg gtaacagtct
    37741 gaatttacat cttaatgtcc gacaaagccg tttactgccg acgctaaatg aagattcagc
    37801 gcttcgcagt tcaatggcgt agttgctgca cccgacgacg ccagagaatg acgaagaaga
    37861 acgtcagcgc attgttcagg tattgcgaga aaccaatggc attgttgccg ggccccgtgg
    37921 cgcggcgaca cgattaggga tgaagcgcac cacgctgctg tcacgaatgc agcgtctggg
    37981 aatctcggtt cgcgaggtgt tgtaatctgc ttttgcagga gtatgcatga gaaacaaact
    38041 ctctttcgac ttgcagttga gcgccagaaa agcggcaatc gctgaacgga ttgccgccca
    38101 taaaattgcc cgcagtaaag tgtcggtctt tttaatggcg atgtccgctg gcgtgtttat
    38161 ggcgatcgga tttacttttt acctttccgt tatcgccgat gccccgtctt cataggcatt
    38221 aacccatctg gtgggcggcc tttgctttac acttggcttt attttgctgg cggtttgcgg
    38281 caccagcctg ttcacctcgt cggtaatgac ggtaatggca aaaagtcggg gcgttattag
    38341 ttggcgaact tggctgatta acgcacttct ggtggcctgc ggtaatctgg caggtattgc
    38401 ctgtttcagt ttgttaatct ggttttccgg gctggtgatg agtgaaaacg cgatgtgggg
    38461 agtcgcgatt ttacactgcg ccgagggcaa aatgcatcat acatttactg aatctgtcag
    38521 cctcggcatt atgtgcaatc tgatggtttg cctggcgctg tggatgagtt attgcggacg
    38581 ttcgttatgc gacaaaatcg tcgccatgat tttgtccatc accctgtttg tcgccagtgg
    38641 ctttgagcac tgtatcgcca atttgtttgt gattccgttc gccattgcca ttcgccattt
    38701 cgcccctacc tccttctggc aactggcgca cagtagcgca gacaattttc cggcactgac
    38761 ggtcagccat tttattaccg ccaatctgct cccggtgatg ctgggtaata ttatcggcgg
    38821 tgcagtgctg gtgagtattt gttatcgggc tatttattta cgtcaggaat cctgaaaaat
    38881 cagcccggcg aaacagttcg tcgggctgaa gattattctt gcgcgatttg cccatcgggc
    38941 caggcgtgga tcacggcttt gatcagcgtc gccaatggaa tggcgaagaa tacgccccag
    39001 aatccccaca aaccaccgaa gatcaccacc gataaaataa tcaccagcgg atgcaggtta
    39061 accgcttcgg agaacaacac cggtactagc aggttgccgt ccagcgcctg aataatcaga
    39121 tacaccgcga agcagctcca gaattcagta cctgcgccaa actggaatag cgccacgcca
    39181 accaccggaa tggtcaccac aaatgcgcca atgtacggaa taagaaccga gaagccgacc
    39241 agcactgcca gcagcagcga atagttcagc ccgaagagca agaaccccag ccaggtggcg
    39301 atccccacca cgatcatctc cagcactttg ccgcggatat agttggtgat ttgttgattc
    39361 atctccttcc acacctgtcc tgccagccca cggttacgcg gcagcacccg gcgaacggcg
    39421 ttcagcatct gctctttgtc tttcagcagg aagaagacca tcaatggcac cagcaccaga
    39481 tagacggcta tggtcagcaa accgaccagc gaggcgaggg aaattttcac caccgaatcg
    39541 cccatggtca gcatccgact gcgcatattt tcggccattg catcaataat gcccgcgtcc
    39601 attaacaccg gatagcggcg cggcaacgtg gcggcaaagt cagaaagctt attgagcatc
    39661 cccggcatat cgcggattaa gtagatgccc tgttgccagg cgataggcag taccacgaac
    39721 gccatcagta gcaatatacc gacaaaaacc accaatacaa tcgacgtcgc ccagcggcgg
    39781 gagcagccaa tagattgcag gcgcacggtt ggccattcca gcaaataagc cagcacaata
    39841 gccaccagca acggagcaag caggccacta aagaaaaaga taatgccaaa tccggcaact
    39901 aaaataacca gcaaggcaat cgcttccggg tcgctaaaac ggcggcgata ccattgcatc
    39961 aacatttcga gcataaagac cttccctgaa cctcaagagc gggattgcga tccgcaattg
    40021 tatcgaaatg tcacaaaaaa gacttcgctt tttatgacgg attcaggaaa ctgaaaagtc
    40081 atttgagtgg gctaatcttc gccgttacac tcaaaggcgg cgaggtggga acgatatttc
    40141 acagtatcgg tcaaatgact atcttcagaa atacaggata gaggttatgt tcaggcagtt
    40201 gaaaaaaaac ctggttgcaa ccctcattgc tgctatgacc attggtcagg tagccccggc
    40261 gtttgccgac agcgcagaca ccttgccgga tatgggaacc tccgcaggaa gcacgctttc
    40321 cattggtcag gaaatgcaga tgggcgacta ttatgtccgc cagctacgcg gcagcgcgcc
    40381 gttaattaat gacccgctgt taacgcaata tattaattcg ctggggatgc gtctggtttc
    40441 gcatgccaat tcggttaaga caccgtttca tttttttctg atcaacaacg acgaaattaa
    40501 cgcctttgct ttctttggcg gcaacgtggt gctgcactct gccctgttcc gttattccga
    40561 taacgaaagt caactggctt cagttatggc gcacgaaatc tcccacgtca cccaacgtca
    40621 cctggcgcga gcgatggaag atcaacaacg cagcgcgccg ctgacctggg tcggcgcgtt
    40681 aggttctatt ttactggcga tggccagtcc gcaggcgggg atggcggcgc tgaccggtac
    40741 actggcggga acgcgccagg ggatgatcag tttcacccag caaaatgaac aggaagcgga
    40801 ccgcattggt attcaggtgc tgcaacgctc gggattcgat ccacaggcga tgccgacctt
    40861 cctggaaaaa ttgctcgatc aggcgcgtta ctcctcgcgc ccgccggaaa ttttattgac
    40921 tcacccgttg ccggaaagtc gtctggcaga tgcccgcaac cgtgctaatc agatgcgccc
    40981 gatggtggtg cagtcgtcgg aagatttcta tctggcgaaa gcgcgcacac tggggatgta
    41041 taattccgga cgtaaccagc tcaccagtga tttgctggat gaatgggcga aaggaaacgt
    41101 tcgtcagcaa cgagcggcgc aatatggtcg tgctttacag gcgatggaag ccaataaata
    41161 cgacgaggcg cgtaaaacgc tgcaaccgtt actggcggca gaacctggca acgcatggta
    41221 tctcgatctg gctaccgata tcgatcttgg gcaaaacaaa gccaatgagg caatcaatcg
    41281 cctgaaaaat gcccgcgatt tacgcaccaa tccggtgttg cagctcaacc tggcgaacgc
    41341 ttatctgcaa ggcggtcaac cacaagaagc ggccaatatt ctgaatcgct acacctttaa
    41401 taataaagat gacagcaacg gctgggattt gctggcacag gcggaagccg cgctaaataa
    41461 ccgcgatcag gagctggctg cgcgagcaga aggttatgcg ctcgccggac gactcgatca
    41521 ggccatttcg ctgttgagta gcgccagttc gcaggtgaaa ttaggcagcc tgcaacaagc
    41581 gcgttacgat gcgcgcatcg accagttgcg ccagctgcag aaacgcttta agccttatac
    41641 caagatgtaa ttcgcattaa aggaagaaca atgaccaaac aggtaaaaat ctaccataac
    41701 ccacgctgtt caaagagccg ggaaacgctg aacctgctga aagaaaacgg cgtggagcca
    41761 gaagtggtgc tctaccttga gacacccgcc gatgcggcaa cgctgcgcga tttgctgaaa
    41821 atgctgggga tgaatagcgc ccgtgaattg atgcgccaga aagaggatct ttataaagaa
    41881 ctaaacctgg ctgacagttc gcttagcgaa gaggcgctga ttcaggcaat ggtggataat
    41941 ccgaagctga tggagcgccc gattatggtc gcgaatggca aagcgcggat tggtcgtcca
    42001 ccggagcagg tactggaaat cgtgggttaa gatgtgctcc atcgctagtt gaagcacatt
    42061 gccggatgcg acgcttgcca cgtcttatcc ggcctacgaa tactatggat taacttcgcg
    42121 taggtcaggc gttcgcgccg catccgacaa taaacacctt atctacaact tcagaatttc
    42181 tttcacaaac ggaatggtca gcttacgttg cgcggtaatc gacgcacgat ccaactgatc
    42241 caacgtcata aatagcgtgc gcatttctct gtcgagccgc ttcagcaaga aacgccccac
    42301 atcttccggc agttcaaaac cacgcaaacg cgcgcgtaac tgtagcgcct gcaacttatc
    42361 ttcatcagaa agtggctgca atttgtagat ctgcccccag tcgagacgcg acgcgagatc
    42421 cggtaatccc agattcaact gccgcggtgg acgatcgccg gtgatcaaca accgtgtttt
    42481 gcccgattcc agaattcgat tgtagagatc gaaaatcgcc atctcccaca actcatcgcc
    42541 tgcaatacac tcaatgttgt cgatacagac cagcgacaaa tgctccatac cgtcgagcac
    42601 ttccggaaca aaccaggtgc gtttatccag cgggacatag cccaccgcat cgccacgctg
    42661 cgacaattcc gcgcaagccg cgtgcagcag atggctgcgc cccgcgcctt cgcgtgccca
    42721 gagatagatg taaccgctat gttcctgacg cagcacgttt tgcagcgcgg ccagtaaaga
    42781 ggagttatcc cccggccaga aacttgcaaa ggtttcgtcg tcaggaagat aaagtggcaa
    42841 agagagctgt gccggtgtgt tcagagatac ctcaaccagg atttcacaaa atcgcgagaa
    42901 gtttaccaca gaatcccatg atgtttgaac cgggcagcaa cactgcccgg tcggtacatt
    42961 atttgtctgt tatatccgcg tcttctgcgt ccagcaccac ttcttcaggg cgcagcacac
    43021 tgatcagttt gaaaatcaaa cttaagccga taccgacgat ggtcgccagc gccatacctt
    43081 ttaactccgc cgcgccgatg tttaccttcg cgccactgac gccgatgatc aaaatcacgg
    43141 aagtcaggat caggttctgt gctttgttgt aatccacttt cgattcgatc aaaacacgaa
    43201 taccggaagc accaatgaca ccataaagca gcagcgaaac gccgcccatc accggcaatg
    43261 ggatcatctg gatagcggca gccagtttac cgacgcagga aagcaggata gcgaaaatcg
    43321 ccgccccgcc gataacccag gtactgtaaa cacgggtgat cgccatcacg ccaatgtttt
    43381 ctccgtaagt ggtatttggc gtagagccaa agaagccgga gatcacggtc gacaggccgt
    43441 tagcaaacat cgaacggtgc agacctggat cgcgcagcag atcttttttg acgatattag
    43501 ccgttactac caggtgccct acgtgttcgg caataaccac taacgccgct ggcagaatag
    43561 tcagaatggc aaaccactcg aagcgcggcg tatagagggt tggcagtgca aaccagtgag
    43621 cattaataat cggcgtggta tcgacaattc ccattgcgaa agagagcgcg taccccacca
    43681 gcacgccaat taaaatcggg ataattgcca ggaaaccacg aaacagcacg gaacctaaaa
    43741 ccgtgaccgc cagggtggta atagagatga tgatggtttt ggagtttggc gtttgccctt
    43801 cagccgggag taaacccgcc ataccggcag ctacgcccgc cagctccaga ccgatgacgg
    43861 caacgattgc gcccattgcc gcaggtggaa acagcacgtc caaccagccg gtccccgctt
    43921 tcttcacgat aaaagaaacc aggcagaaca gcacgccgca cataataaag ccgcccagcg
    43981 cgacttcata ccctaacggc aacagtaaca ataccggtga aataaaggca aagctggaac
    44041 caagataagc cggaattttc cctttacaga tgaagagata cagcagcgtt ccaataccgt
    44101 taaataacag tacagtcgcc gggttaatat gaaataagac gggcaccagg acggttgcac
    44161 caaacatggc gaacaaatgt tgcaaactaa gcgggattgt ctgtaaaagt ggcggtcttt
    44221 cactcacccc gatagcacgg cgcgtcatag tattatcctc tgtattatgt gttataggcg
    44281 ctttactcaa aaaaaagccg actcttaaag tcggctttaa ttatttttat tctttatttc
    44341 gtaccaaaga ttttgtcacc ggcatcgccg aggcccggaa taatgtatcc gtgctcgttc
    44401 agtccctggt caatcgatgc agtatacagt tcgacgtccg ggtgcgcttt ttccagcgca
    44461 gcgatacctt ctggcgcagc gaccagcacc agaactttga tgctgctgca gcccgctttt
    44521 ttcagcaggt cgatggtcgc gataacggaa ccaccggttg ccagcattgg gtcaacgatc
    44581 agcgccatac gctcatcgat gttagaaacc agtttctgga agtacggtac cggctccagc
    44641 gtttcttcat tacggtacat accgacaacg ctgatgcgcg cgctcggaac gttttccagc
    44701 acaccgtcca tcataccaag acccgcacgc agaattggca caacggtaat tttcttacct
    44761 ttgatctggt cgatttctac cgggccgttc cagccttcga tagttacttt ttccgtttcg
    44821 aggtcggcgg tcgcttcgta agtcagcagg ctacccactt cggaagcgag ttcgcgaaag
    44881 cgcttggtgc tgatatcttg ctcacgcatc agtcccagct tgtgtttgac gagtgggtgt
    44941 ttgacttcca cgatcttcat actctttctc ctttgagggg cagccacaaa aaaatcgacg
    45001 gattatacct cctttcttca gggcggcaat attattttcg ttgactttag tcaaaatgat
    45061 aacagtttga gataaagtta ttttatattc aaatggttat gaaagaagat tattccatcc
    45121 gaaaactaac ctttgccctg gcacaagtct tctttcgccg cgcgcccggg gaaaagacgt
    45181 gcaaaaaggt tgtgtaaaac agtctcgcaa acgtttgctt tccctgttag aattgcgccg
    45241 aattttattt ttctaccgca agtaacgcgt ggggacccaa gcagtgaccg ataaaacctc
    45301 tcttagctac aaagatgccg gtgttgatat tgacgcgggt aatgctctgg ttggaagaat
    45361 caaaggcgta gtgaagaaaa cgcgtcgtcc ggaagtgatg ggcggtctgg gcggcttcgg
    45421 tgcgctgtgt gcattgccgc aaaaatatcg tgaacccgtg ctggtttccg gcactgacgg
    45481 cgtaggtacc aagctgcgtc tggcaatgga cttaaaacgt cacgacacca ttggtattga
    45541 tctggtcgcc atgtgcgtta atgacctggt ggtgcaaggt gcggaaccgc tgtttttcct
    45601 cgactattac gcaaccggaa aactggatgt tgataccgct tcagcggtga tcagcggcat
    45661 cgcggaaggt tgtctgcaat cgggctgttc tctggtgggt ggcgaaacgg cagaaatgcc
    45721 ggggatgtat cacggtgaag attacgatgt cgcgggtttc tgcgtgggcg tggtagaaaa
    45781 atcagaaatc atcgacggct ctaaagtcag cgacggcgat gtgctgattg cactcggttc
    45841 cagcggtccg cactcgaacg gctattcgct ggtgcgcaaa attcttgaag tcagcggttg
    45901 tgatccgcaa accaccgaac ttgatggtaa gccattagcc gatcatctgc tggcaccgac
    45961 ccgcatttac gtgaagtcag tgctggagtt gattgaaaag gtcgatgtgc atgccattgc
    46021 gcacctgacc ggcggcggct tctgggaaaa cattccgcgc gtattgccag ataataccca
    46081 ggcagtgatt gatgaatctt cctggcagtg gccggaagtg ttcaactggc tgcaaacggc
    46141 aggtaacgtt gagcgccatg aaatgtatcg caccttcaac tgcggcgtcg ggatgattat
    46201 cgccctgcct gctccggaag tggacaaagc cctcgccctg ctaaatgcca acggtgaaaa
    46261 cgcgtggaaa atcggtatca tcaaagcctc tgattccgaa caacgcgtgg ttatcgaata
    46321 atgaatattg tggtgcttat ttccggcaac ggaagtaatt tacaggcaat tattgacgcc
    46381 tgtaaaacca acaaaattaa aggcaccgta cgggcagttt tcagcaataa ggccgacgcg
    46441 ttcggccttg aacgcgcccg ccaggcgggt attgcaacgc atacgctcat cgccagcgcg
    46501 tttgacagtc gtgaagccta tgaccgggag ttgattcatg aaatcgacat gtacgcaccc
    46561 gatgtggtcg tgctggctgg ttttatgcgc attctcagcc cggcgtttgt ctcccactat
    46621 gccgggcgtt tgctgaacat tcacccttct ctgctgccga aatatcccgg attacacacc
    46681 catcgtcagg cgctggaaaa tggcgatgaa gagcacggta catcggtgca tttcgtcacc
    46741 gatgaactgg acggtggccc ggttatttta caggcgaaag tcccggtatt tgctggtgat
    46801 acggaagatg acatcaccgc ccgcgtgcaa acccaggaac acgctattta tccactggtg
    46861 attagctggt ttgccgatgg tcgtctgaaa atgcacgaaa acgccgcgtg gctggatggt
    46921 caacgtctgc cgccgcaggg ctacgctgcc gacgagtaat gcccccgtag ttaaagcgcc
    46981 agctctgccg ctggcgtttt tcaattcacc tgtaaatcgc aagctccagc agtttttttc
    47041 ccccttttct ggcatagttg gacatctgcc aatattgctc gccataatat ccaggcagtg
    47101 tcccgtgaat aaaacggagt aaaagtggta atgggtcagg aaaagctata catcgaaaaa
    47161 gagctcagtt ggttatcgtt caatgaacgc gtgcttcagg aagcggcgga caaatctaac
    47221 ccgctgattg aaaggatgcg tttcctgggg atctattcca ataaccttga tgagttctat
    47281 aaagtccgct tcgctgaact gaagcgacgc atcattatta gcgaagaaca aggctccaac
    47341 gctcattccc gccatttact gggcaaaatt cagtcccggg tgctgaaagc cgatcaggaa
    47401 ttcgacggcc tctacaacga gctattgctg gagatggcgc gcaaccagat cttcctgatt
    47461 aatgaacgcc agctctccgt caatcaacaa aactggctgc gtcattattt taagcagtat
    47521 ctgcgtcagc acattacgcc gattttaatc aatcctgaca ctgacttagt gcagttcctg
    47581 aaagatgatt acacctatct ggcggtggaa attatccgta gcgatagcat ccgttacgcg
    47641 ctgctggaga tcccatcaga taaagtgccg cgctttgtga atttaccgcc agaagcgccg
    47701 cgtcgacgca agccgatgat tcttctggat aacattctgc gttactgcct tgatgatatt
    47761 ttcaaaggct tctttgatta tgacgcgctg aatgcctatt caatgaagat gacccgcgat
    47821 gccgaatacg atttagtgca tgagatggaa gccagcctga tggagttgat gtcttccagt
    47881 ctcaagcagc gtttaactgc tgagccggtg cgttttgttt atcagcgcga tatgcccaat
    47941 gcgctggttg aagtgttacg cgaaaaactg actatttccc gctacgactc catcgtcccc
    48001 ggcggtcgtt atcataattt taaagacttt attaatttcc ccaatgtcgg caaagccaat
    48061 ctggtgaaca aaccactgcc gcgtttacgc catatttggt ttgataaagc ccagttccgc
    48121 aatggttttg atgccattcg cgaacgcgat gtgttgctct attatcctta tcacaccttt
    48181 gagcatgtgc tggaactgct gcgtcaggct tcgttcgacc cgagcgtact ggcgattaaa
    48241 attaacattt accgcgtggc gaaagattca cgcatcatcg actcgatgat ccacgccgca
    48301 cataacggta agaaagtcac tgtggtggtt gagttacagg cgcgtttcga cgaagaagcc
    48361 aacattcact gggcgaagcg cctgaccgaa gcaggcgtgc acgttatctt ctctgcgccg
    48421 gggctgaaaa ttcacgccaa actgttcctg atttcacgta aagaaaacgg tgaagtggtc
    48481 cgttacgcac acatcgggac cgggaacttt aacgaaaaaa ccgcgcgtct ttatactgac
    48541 tattcgttgc tgaccgcaga tgcgcgtatc accaacgaag tacggcgggt atttaacttt
    48601 attgaaaacc cataccgccc ggtgacattt gattatttaa tggtgtcacc gcaaaactct
    48661 cgccgtctgt tatatgaaat ggtagaccgc gaaatcgcca acgcgcagca agggctgccc
    48721 agtggtatca ccctgaagct aaataacctt gtcgataaag gcctggttga tcgtctgtat
    48781 gcggcctcca gctccggcgt accggttaat ctgctggttc gcggaatgtg ttcgctgatc
    48841 cccaatctgg aaggcattag cgacaacatt cgtgccatca gtattgttga ccgttacctt
    48901 gaacatgacc gggtttatat ttttgaaaat ggcggcgata aaaaggtcta cctttcttcc
    48961 gccgactgga tgacgcgcaa tattgattat cgtattgaag tggcgacacc gctgctcgat
    49021 ccgcgcctga agcagcgggt gctggacatc atcgacatat tgttcagcga tacggtcaaa
    49081 gcacgttata tcgataaaga actcagtaat cgctacgttc cccgcggcaa tcgccgcaaa
    49141 gtacgggcgc agttggcgat ttacgactac atcaaatcac tcgaacaacc tgaataaccc
    49201 tatgccaata cacgataaat cccctcgtcc gcaggagttt gctgcggtcg atcttggttc
    49261 aaacagtttt cacatggtca tagcccgtgt ggtagatggt gccatgcaga ttattggccg
    49321 cctgaaacag cgggtgcatc tggcggacgg cctggggcca gataatatgt tgagtgaaga
    49381 ggcaatgacg cgcggtttga actgtctgtc gctgtttgcc gaacggctac aagggttttc
    49441 tcctgccagc gtctgtatag tcggcaccca tacgctgcgt caggcgctga acgccactga
    49501 ctttctgaaa cgcgcggaaa aggtcattcc ctacccgatt gaaattattt ccggtaatga
    49561 agaagcccgt ctgattttta tgggcgtgga acatacccaa ccggaaaaag gtcgcaaact
    49621 ggttattgat attggcggcg gatctacgga actggtgatt ggtgaaaatt tcgaacctat
    49681 tctcgttgaa agccgccgga tgggttgtgt cagctttgcc cagctttatt ttcctggcgg
    49741 ggtcatcaat aaagagaatt ttcagcgcgc tcgcatggcg gcagcacaaa aactggaaac
    49801 tttaacctgg caattccgta ttcagggctg gaacgttgca atgggcgctt ccggtaccat
    49861 aaaagccgcc catgaagtgt taatggaaat gggcgagaaa gacgggataa ttaccccgga
    49921 acgtctggaa aaactggtaa aagaaatttt acgtcaccgt aatttcgcat cgctgagttt
    49981 accgggtctt tccgaagagc ggaaaacagt cttcgttcct ggactggcga ttttatgtgg
    50041 tgtgtttgat gctttagcca tccgtgaact gcgcctttct gacggggcgt tacgcgaagg
    50101 cgtactgtat gaaatggaag gacgtttccg tcatcaggat gtgcgtagtc gcaccgccag
    50161 cagcctcgcc aaccagtatc acatagacag cgaacaggcc cgacgagtgc tggataccac
    50221 tatgcaaatg tacgaacagt ggcgggaaca gcaaccgaag ctggcgcatc cgcaactgga
    50281 ggcgctactg cgatgggccg ccatgctgca tgaggtcggg ttgaatatca accacagcgg
    50341 tttgcatcgc cactccgctt atattctgca aaacagtgac ttgccgggtt ttaatcagga
    50401 acagcagctg atgatggcga cactggtgcg ctatcaccgt aaagcgatta agctcgacga
    50461 tctgccgcgc tttaccttgt ttaagaagaa acagttcctg ccactgatac agctattgcg
    50521 ccttggcgta ttactaaaca atcaacgtca ggcaaccacc acaccgccaa cattgacact
    50581 gattactgat gacagtcact ggacactgcg tttcccgcat gactggttta gtcagaatgc
    50641 gctggtactg cttgatctgg aaaaggagca agaatactgg gaaggcgtgg ctggctggcg
    50701 gttgaaaatt gaagaagaaa gtaccccaga aatcgccgct taatgctttg cgggccgacg
    50761 agaaatgtcg gcccgcatta ttcaggcact ttcgcgaatg ggttcgattt cattcagcgt
    50821 atcaattaac ggttgcggct taccaataag ataaccctgc atataatcga tccccaaaga
    50881 gagcaccgcc tcgcggatct cttcgttttc aacgtactct gccactaccc gcattttctt
    50941 cattcgcgcc agatggcaaa tcgacgccac aatctgataa tccagactat ttgacacaat
    51001 attgctgata aagctgccgt caattttaag cagatcggca ttcacatttt taagccgtgc
    51061 atagctggcg tagccggtgc cgaaatcatc aatcgcaatc tggcagccta attcctgaag
    51121 atgctgcaag gtgatttgcg cctgcttaac attggtcaga gcattacttt cggtgacttc
    51181 aaaaataagt tgccacgctt caatctgata tttagccagc aactgactga cttcaacagg
    51241 aaaacgagcc tgacataccg aggttggaga cagattaata gcaaaacggt gagcgggcat
    51301 cttcgctctg ttttcagcca taaattgcag cgtatgctca atgacccaca tgtcgatact
    51361 cgacgataaa ccaaattcgt gcgcgaccgg caagaagcta ccggggccga tcagttcatc
    51421 attctcacct ttcatgcgca gaagaatttc atggtaaaca tcaccacgca taccggtaat
    51481 cggctgggcc atcaggaaaa aatggttgtg ttccagcgcc tgctgtagtc gattcatcat
    51541 cgcgacttta tctttcaatt cgcgttgcaa atacattgcc ccgcaacgct gcatattttc
    51601 cggggcgttg gtcacgatgg aaagttcggc gaccgtattt agctctccca gcagcaggta
    51661 gatatgattc actggcgagc gcacatagca gtaactgacg ccaatctgcg gttgcatcgg
    51721 catcccatcc caaaagaaac gaaattgctt gagatgacta tccagtgcgg taatgcgctc
    51781 ctggtgcgat tctgtattca gtcgcagcgc gagatcgtta cccgaaagct gataaacatc
    51841 ttcacccggt tccagcaagg gtgacagcca gtgagaaagt ttttgcttgt
//
LOCUS       DAEBXN010000018        50050 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.018, whole genome shotgun sequence.
ACCESSION   DAEBXN010000018 DAEBXN010000000
VERSION     DAEBXN010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 50050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50050
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.018"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            191..694
                     /locus_tag="KJL99_001022"
     CDS             191..694
                     /locus_tag="KJL99_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HBD4435841.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(774..1025)
                     /locus_tag="KJL99_001023"
     CDS             complement(774..1025)
                     /locus_tag="KJL99_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HBD4435842.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(1140..1226)
                     /gene="azuC"
                     /locus_tag="KJL99_001024"
     CDS             complement(1140..1226)
                     /gene="azuC"
                     /locus_tag="KJL99_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HBD4435843.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            1489..1812
                     /locus_tag="KJL99_001025"
     CDS             1489..1812
                     /locus_tag="KJL99_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HBD4435844.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            1983..2480
                     /gene="ftnA"
                     /locus_tag="KJL99_001026"
     CDS             1983..2480
                     /gene="ftnA"
                     /locus_tag="KJL99_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HBD4435845.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(2517..2756)
                     /locus_tag="KJL99_001027"
     CDS             complement(2517..2756)
                     /locus_tag="KJL99_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HBD4435846.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGVTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            2946..4157
                     /gene="tyrP"
                     /locus_tag="KJL99_001028"
     CDS             2946..4157
                     /gene="tyrP"
                     /locus_tag="KJL99_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HBD4435847.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(4219..4884)
                     /gene="yecA"
                     /locus_tag="KJL99_001029"
     CDS             complement(4219..4884)
                     /gene="yecA"
                     /locus_tag="KJL99_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HBD4435848.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(5080..5166)
                     /locus_tag="KJL99_001030"
     tRNA            complement(5080..5166)
                     /locus_tag="KJL99_001030"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5130..5132),aa:Leu,seq:taa)
     gene            complement(5179..5252)
                     /locus_tag="KJL99_001031"
     tRNA            complement(5179..5252)
                     /locus_tag="KJL99_001031"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5218..5220),aa:Cys,seq:gca)
     gene            complement(5307..5382)
                     /locus_tag="KJL99_001032"
     tRNA            complement(5307..5382)
                     /locus_tag="KJL99_001032"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5347..5349),aa:Gly,seq:gcc)
     gene            complement(5534..6082)
                     /gene="pgsA"
                     /locus_tag="KJL99_001033"
     CDS             complement(5534..6082)
                     /gene="pgsA"
                     /locus_tag="KJL99_001033"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HBD4435849.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYVGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(6139..7971)
                     /gene="uvrC"
                     /locus_tag="KJL99_001034"
     CDS             complement(6139..7971)
                     /gene="uvrC"
                     /locus_tag="KJL99_001034"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HBD4435850.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAACIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(7968..8624)
                     /gene="uvrY"
                     /locus_tag="KJL99_001035"
     CDS             complement(7968..8624)
                     /gene="uvrY"
                     /locus_tag="KJL99_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HBD4435851.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            8920..9096
                     /locus_tag="KJL99_001036"
     CDS             8920..9096
                     /locus_tag="KJL99_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4435852.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTT
                     NFHCNMTRKINEYA"
     gene            9083..9307
                     /locus_tag="KJL99_001037"
     CDS             9083..9307
                     /locus_tag="KJL99_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HBD4435853.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(9340..9960)
                     /gene="sdiA"
                     /locus_tag="KJL99_001038"
                     /pseudo
     CDS             complement(9340..9960)
                     /gene="sdiA"
                     /locus_tag="KJL99_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
     gene            complement(10190..10942)
                     /gene="yecC"
                     /locus_tag="KJL99_001039"
     CDS             complement(10190..10942)
                     /gene="yecC"
                     /locus_tag="KJL99_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HBD4435854.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKLLLQ"
     gene            complement(10939..11607)
                     /gene="tcyL"
                     /locus_tag="KJL99_001040"
     CDS             complement(10939..11607)
                     /gene="tcyL"
                     /locus_tag="KJL99_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HBD4435855.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(11622..12608)
                     /gene="dcyD"
                     /locus_tag="KJL99_001041"
     CDS             complement(11622..12608)
                     /gene="dcyD"
                     /locus_tag="KJL99_001041"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HBD4435856.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDEVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(12713..13513)
                     /gene="tcyJ"
                     /locus_tag="KJL99_001042"
     CDS             complement(12713..13513)
                     /gene="tcyJ"
                     /locus_tag="KJL99_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HBD4435857.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKESGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(13601..14152)
                     /gene="fliZ"
                     /locus_tag="KJL99_001043"
     CDS             complement(13601..14152)
                     /gene="fliZ"
                     /locus_tag="KJL99_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HBD4435858.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQQSWAALCRFCGDLHCKTQSDFFDIIGFKKFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(14198..14917)
                     /gene="fliA"
                     /locus_tag="KJL99_001044"
                     /pseudo
     CDS             complement(14198..14917)
                     /gene="fliA"
                     /locus_tag="KJL99_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
     gene            complement(15082..16734)
                     /gene="fliC"
                     /locus_tag="KJL99_001045"
     CDS             complement(15082..16734)
                     /gene="fliC"
                     /locus_tag="KJL99_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HBD4435859.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGTAASATNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDT
                     AKGTITIDGSAQDVQISSDGKITASNGDKLYIDTTGRLTKNGSGASLTEASLSTLAAN
                     NTKATTIDIGGTSISFTGNSTTPDTITYSVTGAKVDQAAFDKAVSTSGNNVDFTTAGY
                     SVNGTTGAVTKGVDSVYVDNNEALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTT
                     DAETKAATTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRI
                     QDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            17000..18406
                     /gene="fliD"
                     /locus_tag="KJL99_001046"
     CDS             17000..18406
                     /gene="fliD"
                     /locus_tag="KJL99_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HBD4435860.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGDDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKVQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            18431..18841
                     /gene="fliS"
                     /locus_tag="KJL99_001047"
     CDS             18431..18841
                     /gene="fliS"
                     /locus_tag="KJL99_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HBD4435861.1"
                     /translation="MYAAKGTQAYAQIGVESTVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSSIQDPV"
     gene            18841..19206
                     /gene="fliT"
                     /locus_tag="KJL99_001048"
     CDS             18841..19206
                     /gene="fliT"
                     /locus_tag="KJL99_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HBD4435862.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            19284..20771
                     /gene="amyA"
                     /locus_tag="KJL99_001049"
     CDS             19284..20771
                     /gene="amyA"
                     /locus_tag="KJL99_001049"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HBD4435863.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAECWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(20805..21218)
                     /locus_tag="KJL99_001050"
     CDS             complement(20805..21218)
                     /locus_tag="KJL99_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBD4435864.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRGIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            21405..22610
                     /gene="yedE"
                     /locus_tag="KJL99_001051"
     CDS             21405..22610
                     /gene="yedE"
                     /locus_tag="KJL99_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HBD4435865.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRE
                     APQTAKEIA"
     gene            22607..22840
                     /gene="yedF"
                     /locus_tag="KJL99_001052"
     CDS             22607..22840
                     /gene="yedF"
                     /locus_tag="KJL99_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HBD4435866.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            22947..23617
                     /locus_tag="KJL99_001053"
                     /pseudo
     CDS             22947..23617
                     /locus_tag="KJL99_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(23657..24865)
                     /locus_tag="KJL99_001054"
                     /pseudo
     CDS             complement(23657..24865)
                     /locus_tag="KJL99_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            24933..25548
                     /gene="tnpA"
                     /locus_tag="KJL99_001055"
                     /pseudo
     CDS             24933..25548
                     /gene="tnpA"
                     /locus_tag="KJL99_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            25876..26958
                     /locus_tag="KJL99_001056"
     CDS             25876..26958
                     /locus_tag="KJL99_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HBD4435867.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLIEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
     gene            complement(27447..28245)
                     /locus_tag="KJL99_001057"
                     /pseudo
     CDS             complement(27447..28245)
                     /locus_tag="KJL99_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(28255..28806)
                     /gene="ybcL"
                     /locus_tag="KJL99_001058"
     CDS             complement(28255..28806)
                     /gene="ybcL"
                     /locus_tag="KJL99_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HBD4435868.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPTDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(28975..29307)
                     /gene="emrE"
                     /locus_tag="KJL99_001059"
     CDS             complement(28975..29307)
                     /gene="emrE"
                     /locus_tag="KJL99_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HBD4435869.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(29652..29966)
                     /gene="fliE"
                     /locus_tag="KJL99_001060"
     CDS             complement(29652..29966)
                     /gene="fliE"
                     /locus_tag="KJL99_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HBD4435870.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            30180..31839
                     /gene="fliF"
                     /locus_tag="KJL99_001061"
                     /pseudo
     CDS             30180..31839
                     /gene="fliF"
                     /locus_tag="KJL99_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
     gene            31832..32827
                     /gene="fliG"
                     /locus_tag="KJL99_001062"
     CDS             31832..32827
                     /gene="fliG"
                     /locus_tag="KJL99_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HBD4435871.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLRRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLAKRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            32820..33506
                     /gene="fliH"
                     /locus_tag="KJL99_001063"
     CDS             32820..33506
                     /gene="fliH"
                     /locus_tag="KJL99_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HBD4435872.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            33506..34879
                     /gene="fliI"
                     /locus_tag="KJL99_001064"
     CDS             33506..34879
                     /gene="fliI"
                     /locus_tag="KJL99_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HBD4435873.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            34901..35242
                     /gene="fliJ"
                     /locus_tag="KJL99_001065"
                     /pseudo
     CDS             34901..35242
                     /gene="fliJ"
                     /locus_tag="KJL99_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
     gene            35239..36366
                     /gene="fliK"
                     /locus_tag="KJL99_001066"
     CDS             35239..36366
                     /gene="fliK"
                     /locus_tag="KJL99_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HBD4435874.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLTLLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTADASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            36471..36935
                     /gene="fliL"
                     /locus_tag="KJL99_001067"
     CDS             36471..36935
                     /gene="fliL"
                     /locus_tag="KJL99_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HBD4435875.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            36940..37944
                     /gene="fliM"
                     /locus_tag="KJL99_001068"
     CDS             36940..37944
                     /gene="fliM"
                     /locus_tag="KJL99_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HBD4435876.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASISGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVEFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            37941..38354
                     /gene="fliN"
                     /locus_tag="KJL99_001069"
     CDS             37941..38354
                     /gene="fliN"
                     /locus_tag="KJL99_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HBD4435877.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSEKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            38357..38722
                     /gene="fliO"
                     /locus_tag="KJL99_001070"
     CDS             38357..38722
                     /gene="fliO"
                     /locus_tag="KJL99_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HBD4435878.1"
                     /translation="MNNHATVQSSAPVSAEPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            38722..39459
                     /gene="fliP"
                     /locus_tag="KJL99_001071"
     CDS             38722..39459
                     /gene="fliP"
                     /locus_tag="KJL99_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HBD4435879.1"
                     /translation="MRRLFSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            39469..39738
                     /gene="fliQ"
                     /locus_tag="KJL99_001072"
     CDS             39469..39738
                     /gene="fliQ"
                     /locus_tag="KJL99_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HBD4435880.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            39747..40532
                     /gene="fliR"
                     /locus_tag="KJL99_001073"
     CDS             39747..40532
                     /gene="fliR"
                     /locus_tag="KJL99_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HBD4435881.1"
                     /translation="MMQETSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPGSHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     SEPLNSNAFLAPTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            40817..41440
                     /gene="rcsA"
                     /locus_tag="KJL99_001074"
     CDS             40817..41440
                     /gene="rcsA"
                     /locus_tag="KJL99_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HBD4435882.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(41484..41672)
                     /gene="dsrB"
                     /locus_tag="KJL99_001075"
     CDS             complement(41484..41672)
                     /gene="dsrB"
                     /locus_tag="KJL99_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HBD4435883.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVALEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            41835..42062
                     /gene="yodD"
                     /locus_tag="KJL99_001076"
     CDS             41835..42062
                     /gene="yodD"
                     /locus_tag="KJL99_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HBD4435884.1"
                     /translation="MKTAKVYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            42360..43175
                     /locus_tag="KJL99_001077"
                     /pseudo
     CDS             42360..43175
                     /locus_tag="KJL99_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(43172..44866)
                     /gene="yedQ"
                     /locus_tag="KJL99_001078"
                     /pseudo
     CDS             complement(43172..44866)
                     /gene="yedQ"
                     /locus_tag="KJL99_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
     gene            complement(45037..45219)
                     /locus_tag="KJL99_001079"
     CDS             complement(45037..45219)
                     /locus_tag="KJL99_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HBD4435885.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
     gene            complement(45298..46215)
                     /locus_tag="KJL99_001080"
     CDS             complement(45298..46215)
                     /locus_tag="KJL99_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HBD4435886.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            46388..47308
                     /gene="yedA"
                     /locus_tag="KJL99_001081"
     CDS             46388..47308
                     /gene="yedA"
                     /locus_tag="KJL99_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HBD4435887.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVGSWPPLMMAGVRF
                     QAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     IATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(47297..47767)
                     /gene="vsr"
                     /locus_tag="KJL99_001082"
     CDS             complement(47297..47767)
                     /gene="vsr"
                     /locus_tag="KJL99_001082"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HBD4435888.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRRREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(47748..49166)
                     /gene="dcm"
                     /locus_tag="KJL99_001083"
     CDS             complement(47748..49166)
                     /gene="dcm"
                     /locus_tag="KJL99_001083"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HBD4435889.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKMLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(49233..49773)
                     /locus_tag="KJL99_001084"
                     /pseudo
     CDS             complement(49233..49773)
                     /locus_tag="KJL99_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
     gene            complement(49813..>50050)
                     /locus_tag="KJL99_001085"
     CDS             complement(49813..>50050)
                     /locus_tag="KJL99_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4435890.1"
                     /translation="WAFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWL
                     GALVGFLFMALVGALLGGIAWYTRPRPARCRRYE"
BASE COUNT        12478 a        12832 c        12182 g        12558 t
ORIGIN      
        1 aaatatataa acttgatgat ttaagcattt tcttataacc gttcagacgt tattcttatt
       61 tcagatcatc gtcagaattg actccacgat cacatttcgg accggcagaa aggaattatt
      121 ctgcaaacag taattatggt gttttgattt atcttgcacc tctccacttc tggatataag
      181 gatattaggt atggcaaccg ctggaatgct tctcaaactc aactctcaaa tgaaccgcga
      241 gttttacgca tccaatctct accttcacct gagtaactgg tgttctgaac agagtctgaa
      301 cggcaccgcc actttccttc gcgcccaggc acagagtaat gtgacccaaa tgatgcgcat
      361 gtttaacttt atgaagagtg tcggcgctac ccccatcgtt aaagccattg atgttccagg
      421 tgaaaaactg aactctctgg aagaactgtt ccaaaaaacg atggaagaat acgagcaacg
      481 ttcaagcact ctggcgcagt tagccgatga agcgaaagaa ctgaatgatg attcaaccgt
      541 caatttcctg cgcgatctgg aaaaagaaca gcagcatgat ggcctgttgc tgcaaaccat
      601 tcttgatgaa gtgcgcagtg cgaaacttgc gggtatgtgc cctgtgcaga ccgaccaaca
      661 tgttctgaat gtcgtgtcac accagctgca ttgatcatac atcggcgcta atacattgcg
      721 ccgatgaagt ttttgagaaa ccgctgcctc atctgtttga agcagcggtt tttttaatgg
      781 gattcaccct gtggggtaaa cttgagttca atcagcgcga tggctttttg gattgcccgc
      841 atggtgaccg ggtctgcggc agcgggatga tgagtaaagt cgatattctt cagctgactg
      901 gacatttttt cacgaacttc aacgggcgcg attacatcga gaacatccag aatttgtttg
      961 ataaccaact ggcaagctac cacatcagaa accagttcct gatcggcatt cagcggctgg
     1021 gacatcgtaa actcctgata gcattttgaa agccgttata gtagcggctt cacatcttca
     1081 gcgatagtca catccaccgt catcaggaca caaaaaaacc tgccggagca ggttttttgt
     1141 tatcggaaca tattgcctgg cggtacgtct ttgaacgtct tgcaatagtt attgaacata
     1201 cttttcagga ttttgcgcag tttcatcgcg gcactccgac catttgttat acaggtgtta
     1261 ttgtctttgc gcttataata tgacaaccat cacaaaaatc aatctttatg tgatacaaat
     1321 cacataaata cccctttaat gttataaaaa tgataatcaa aaaacagccc ccctatttct
     1381 gacacctaca gatggcaaga aatagcgcct gccaggcgtc ttttccggcc attgtcgcag
     1441 cgctgtaacg cgtaaaatag tgctttctct tactcttctg gctggaccat gagacttctg
     1501 attctgactc tttcattaat aacgctggcg gggtgtacgg ttactcgcca ggcccatgtg
     1561 agcgaagttg acgcggcaac aggtattgta cggttggttt atgaccaggc ttttttgcag
     1621 catgcccata ccgatcgtta tgtcagtcgc gggatcgccg atcgcgcatg ccagcaggaa
     1681 ggctataccc acgcgatccc ctttggtcag ccagtaggca actgtagcct ttttgcgggt
     1741 tccttatgtt tgaatacgga attcactttg tcatatcagt gccatcattc cgccttccct
     1801 gtctttctct aaatatttta atcagcgagg ggatcttcgc tgattaaaga aatagcggta
     1861 atgcgtttta ttcccattcg tatttttaat aattgaagtt tatattttac cttttgcaaa
     1921 taataaaata acaaattata gtgacgccac agcaacaaat ataacctttg tggagcacta
     1981 tcatgctgaa accagaaatg attgaaaaac ttaatgagca gatgaacctg gaactgtact
     2041 cttcactgct ttatcagcaa atgagcgcct ggtgcagcta tcataccttc gaaggtgctg
     2101 ccgcgttcct gcgccgtcac gcccaggaag agatgacgca tatgcagcgt ctgtttgatt
     2161 acctgactga taccggcaat ttaccgcgta ttaataccgt tgaatctccg tttgctgaat
     2221 attcctcact tgatgaatta ttccaggaaa cctataaaca cgaacaatta atcacccaga
     2281 aaattaacga actggctcat gctgcaatga ccaatcagga ctacccaaca tttaatttcc
     2341 tgcagtggta tgtttctgag cagcatgaag aagagaaact gttcaaatcg attattgata
     2401 aattaagcct ggcaggcaaa agcggcgaag gtctgtattt tatcgacaaa gaactctcta
     2461 ccctcgacac acaaaactaa tgctctccgg cggcaggttt ctgccgccgt ttcaaatcag
     2521 tgacggcaaa tcttactctg atcggtcgaa aaaccttctt ctgaaggtat aaatttgcct
     2581 tttgctgcca gaaacgctac cagctctccc gctgtcaccc cttctgccga gcacgtgtga
     2641 aaacgtgcct gttcaccaaa acgcgcttta atcgcagctt ccagactggc atgcgtatat
     2701 tgctcgcctg attcaatcat catatttaac acttcatgac cgtgaataga gtccatcgtt
     2761 cctcctcaaa aaaagcctag cgtagcgatt accgcttatg aagactttgc gccagcgcag
     2821 gactgaatgc ttttattgta catttatatt tacacaatat gtaacgtcgg tttgacgaag
     2881 cagccgttat gccttaacct gcgccgcaga tatcactcat aaagatcgtc aggacagaag
     2941 aaagcgtgaa aaacagaacc ctgggaagtg tttttatcgt ggcgggaacc acaattggcg
     3001 caggcatgct ggcaatgccg ctggctgcgg ccggtgttgg ttttagcgtt acgttaatct
     3061 tgttgattgg gctttgggcg ttgatgtgct acacggcgct attactgctg gaggtgtacc
     3121 agcatgttcc ggcagatacc ggtctgggca cgctggcaaa acgctatctg ggacgctacg
     3181 gtcaatggct gacgggcttc agtatgatgt tcttaatgta tgctctgact gcagcataca
     3241 tcagcggtgc cggtgaattg ttggcctcca gcatcagcga ctggacaggt atttcaatgt
     3301 cggcaaccgc tggcgtgctg ttgttcactt ttgttgccgg tggcgtggtt tgtgttggaa
     3361 catcgctggt cgatttattt aaccgttttc tgttcagcgc caaaattatt tttctggtgg
     3421 tgatgctggt attgctgcta ccgcatattc acaaagtgaa tcttttaacc ctgccgttgc
     3481 aacaggggct ggcactgtct gcaatcccgg tgatttttac gtcgtttggt tttcacggta
     3541 gcgtgccgag tattgtcagc tatatggatg gcaacattcg taagctacgc tgggtgttta
     3601 taatcggtag tgccatcccc ctggtggcat atattttctg gcaggtggcg acgcttggca
     3661 gcattgattc aacaaccttt atgggattgc tggctaatca tgctggatta aacgggctgt
     3721 tacaggcgtt acgcgaaatg gtggcctctc cgcatgttga gctggcggtg catttatttg
     3781 ctgatttagc cctcgccacg tcatttctcg gcgttgcgtt aggcttattt gattatctgg
     3841 ctgatttgtt tcagcgttca aataccgttg gtggacgatt gcaaactggt gcaattacct
     3901 ttctgccgcc gttggcgttt gcactgtttt atccacgagg atttgtgatg gcgctgggtt
     3961 acgccggtgt ggcgctggcg gtactggcat tgattatccc ttcactattg acctggcaaa
     4021 gcagaaagca caatcctcag gcgggttacc gggtcaaagg tggtcgtccg gcgctggtgg
     4081 tggtgtttct ctgtggtatt gctgtgattg gcgtgcaatt tttgattgcg gcagggttgt
     4141 taccagaagt ggggtgatca gatagcctca aattccttat tgggtgccag aattaacgct
     4201 gacacccaat gtgacctctt aatgcaggca gcactgctta aatttcttac cactgccgca
     4261 cggacaagga tcgttacgcc ccggtttctc ttctgctttg atcggttgct gaacagcttt
     4321 ttcctgcgga tgcgccatcc agtatgcatg taaatcaagc gccgccagtc gaatggcatc
     4381 tacgctctct tcaaacgctt ctggcgacat tttctctacc cgctcgaagt tttcctcagt
     4441 accgtgcagc gcaatcgcct ccagcgctgg ttttaaagaa tcgggcaacg ttgaccagtc
     4501 agaaagtgcc acgccccgca tatagccaaa gcaccactcc tcaacaatcg tcagctcgct
     4561 gccatcaact tctcgcaagc cgaataacgg ctcaaactgc tccgggaatt cgttcagacg
     4621 ctctgcggta tcggccatat gttgaaaagc caggttcata aagcgcgtca tctctttctc
     4681 tgacgcccaa cgcggcacat agtcagcccc accccacacg gcaaccagcc actgttctgg
     4741 ttcaatctct tgcggagaac tcaacaccgc cgtcaataaa ccgtccagct ccgccacatc
     4801 aaggatggcg tggtcagtgt tgtatttggt cagaatatcg tccagccatt ccaactcact
     4861 ttcgtttaac ggtcccgttt tcatacgctt ttccttgtgg atctcaactc gccagcacct
     4921 atcttacatg ccggtccgta tcagagatac tttttgagtg gctttgccgg tgattaaaaa
     4981 ttaaggaggg tgtaacgaca agttgcaggc acaaaaaaac cacccgaagg tggtttcacg
     5041 acactgctta ttactttgat tttattctta tctttcccat ggtacccgga gcgggacttg
     5101 aacccgcaca gcgcgaacgc cgagggattt taaatccctt gtgtctaccg attccaccat
     5161 ccgggctcgg gaagaaagtg gaggcgcgtt ccggagtcga accggactag acggatttgc
     5221 aatccgctac ataaccgctt tgttaacgcg ccaaattctt caggcctttc agccagacat
     5281 ccgcttgacg ccgatgtctt ttaaactgga gcgggaaacg agactcgaac tcgagacctc
     5341 gaccttggca aggtcgtgct ctaccaactg agctattccc gcattcatca agcaatcagt
     5401 taatcacttg attttattat cgtctggcaa tcagtgccgc cgttcgatgc gttgcattct
     5461 acttacctgg cgcgatgagt caacgatatt tttcaccact tttgatcgtt tgctgaaaat
     5521 tacgccgaaa cgatcactga tcaagcaaat ctgcacgcgc agcgctcaaa tattgcaaca
     5581 ttgaccacag agtcagtacc gcagccacaa agaaaagtgc aataccgacg tactcaaccc
     5641 aaatgttcgg acgccacagc agccaggcca acgccaccat ctgggcagtg gttttcactt
     5701 tcccaatcca ggagacggcc acgctactgc gtttacccaa ctccgccatc cattcgcgta
     5761 gcgcagaaat aataatttca cgggcgatca tcgttgccgc cggtaatgtc acccaccagc
     5821 tgtgataatg ctcggttacc agcaccatgg cgatagccac gagaacttta tctgccacag
     5881 ggtcaaggaa agcaccaaac cgggtactct ggttccagcg gcgtgccaga aaaccatcga
     5941 accagtcagt caccgccgcg acgcagaaaa tgagcgcggc ggcaaacggc gaccaggtga
     6001 caggcagata aaagaccaat acaaagaatg ggataaggat gacacggaac agtgtaagca
     6061 acgtagggat attaaattgc ataatgacgg gtaactatct gttgtcagta agattacccc
     6121 tatgttgcta cagagacatc aatgtttcaa cgaccagaag atcttttctg ccagaccttg
     6181 cgaaataccc ggcacttttg caatttcctc gacgctggcg ttacgtaaac cttgcaaacc
     6241 gcccatatat ttcaacaaca tttgccgacg ttttggcccg acgccttcaa tggtttccag
     6301 ggaactggta tttttgacct tcgcccgttt tttacggtgc ccgccaatcg cgtgatcatg
     6361 tgattcatcg cgaatatgct ggataacatg cagcgcgggt gaatctggcg gcaaactaaa
     6421 tccctcacct tccggctcaa agaacagcgt ttccagtcca gccttacgat ctgctccttt
     6481 ggcaacgcca agtagcagcg gatgattttt atcccatgag acatccagtt cggcgaagac
     6541 atttttcgcc tgcgcaagct ggcctttgcc gccgtcgata aggatcacat ccgggatctt
     6601 actgtcgtca atggctttac cataacgccg acgcagcacc tgattcatcg ccgcataatc
     6661 atcgcccggc gtgatgcctg taatgttata gcgccgatac tccgcacgca gcgggccgtt
     6721 agcatcaaac accacacagg aagcgacggt ttgttcgccc atggtatggc tgatgtcaaa
     6781 gcactccatc cgcttcactt ccggcaattt caacacgctg gcaagcgcgg tcagtcgctg
     6841 gtgaacggta gattgctgcg aaagtttgct ggttaaggcc gtcgccgcat tggtgcgcgc
     6901 gagtttcaga taacgcgccc tatcgccgcg aggtttggtt tgaacattaa tcttgcgtcc
     6961 cgccagttct gaaagggaat cggcgagcag cgttttatcg ctaagattaa aatcgagcag
     7021 gatctcaccc ggtaaggtgc gcatctggct gccttgtaaa tagaactgac ctacgaaggt
     7081 ttctaccacc tcgctcagtt ccgtaccgcc aggcactttc gggaaatagc tgcggctgcc
     7141 gagcactttg ccctgacgaa tgaacaatac gtggacacaa gccatgcccg catcgaacgc
     7201 cacaccaata acgtcaaggt cgtcgccggt attggaaaca aactgttttt cggtgacgcg
     7261 tcgcaccgcc tgaatttggt cgcgaataca tgccgcttct tcaaactcca gattctggct
     7321 ggcagtttcc atacggctaa tgagttgcgt aagcacctga tcatctttgc cagacaaaaa
     7381 caggcgcaca tactcgacct gctgagcgta ttcttcttca ctcaccagtc cttcaacgca
     7441 cggtcccaga cagcgcccta tctggtattg cagacacgga cgcgagcgat tgcgataaac
     7501 actattttcg cactggcgaa tggggaaaat cttttgcagt agcgccagtg tttcacgtac
     7561 ggcatagcca ttcgggaacg ggccgaaata ttcacctttg gcatgctttg caccacgatg
     7621 catcgccaga cgcgggtggg tatcgccact caggaagata aaaggataag atttatcatc
     7681 gcgtagcaaa acgttgtaac gcggctgata gagtttgatg tagttgtgtt ccagcaacag
     7741 cgcttcggtt tctgtatgcg taaccgttac atcaatttgc tggatctggg cgaccagcgc
     7801 ttcggttttg cgcgaagcga ggttgctgcg gaaatagctg gaaagccgtt ttttcaggtc
     7861 tttcgctttg ccgacataga taaccgtacc accagcatcg tacatgcgat aaacgcctgg
     7921 ctggctggtt acggttttta aaaacgcttt tgcgtcaaac tgatcactca ctgacttgat
     7981 aatgtctccg cattacacag accatggcga attgccaggt gagtcagctc aacatcgcca
     8041 tgaatgttta gtttactgaa catacgatag cggtagctgt tcaccgtttt cggactgaga
     8101 ttgagctgtt ctgagatctc attgaccttc tggcccttag tgatcatcag cataatctgc
     8161 aattcacgtt cagacaaact ggcaaatggg ctttctgttt tttctggttc gatctggctt
     8221 aacgccattt gttgagcgat atcagaagca atgtaacgct gccctgaata gacagaacga
     8281 atcgcactca cgacttcctg cggagccgcg cctttgctga ggtagccagc agcacctgcc
     8341 tgcatgactt tcgctggtaa agggttttct gtatggaccg taagcatgat gattttaaca
     8401 tcagctgtgg aacgcgcgat tttacgcgtc gcctcaagac cgccaatgcc cggcatactc
     8461 atgtccatta gcaccacgtc aacggcattt gtccggcacc acttaacggc gtcttcaccg
     8521 cacgatgcct ctccgacgac ttttataccc ttaatatctt ccagaatgcg tcgtatccct
     8581 gcgcgcacca gttcgtggtc atcaacaagt agaacgttga tcaaaggaat atctccagaa
     8641 atagggataa cgctactgat agttagtcat tcgtatatta gcggttttta ttgcaacttt
     8701 gaaacgttaa aaatggtggg tattcgattt ttctctcgtt tttgtcaatt cgattgtcca
     8761 cagttatggt cacgcccgtc accaacggct taccggatcg tttcacagta agccctacga
     8821 tgaaatgtga caaaaattga ctttattcag caaaaatgaa aatcagcccg ctgaatatgt
     8881 aacattaatt aacccgaaca atacgggcaa aaaacattca tgataaaaat atttattggt
     8941 cattatatta acgtatttta tagcactgcc gatatcacgc tcaaaaaaca accactgcta
     9001 tttttagcaa agcttatggt atactccgcc gccttaacat ttttcaccac aaattttcat
     9061 tgcaacatga cgaggaaaat aaatgagtac gcctgatttt tctactgccg agaataatca
     9121 agaactggca aatgaagtct cctgcctgaa agcgatgctg acgctgatgc tgcaggcgat
     9181 gggacaagct gacgcgggcc gcgtgatgtt aaagatggaa aaacagcttg cgctgatcga
     9241 agacgaaacc caggctgcag tattttccaa aacggttaag caaattaaac aagcctaccg
     9301 tcagtaatat aaaaccggct gagagcgtgc ctttcagccg gttattgaca aaatcatcgc
     9361 tatctctgct gatgtcttcc cttccgccgt ccatttcaga atttcttttt cgcgcttgct
     9421 gaaattcatc tctggcgtca tcactatttc atcatttaaa cgcatcagag ccatcagact
     9481 ttcgcgcacc agtaactgca tttttaattg cagttcatca ctaagaatgg gtatttcgcg
     9541 cgtgctgcaa cgggaaaagg acaaaaagcc cagcgcccga tttggcagca ttaaatactg
     9601 agtgacaccg cggcgtaaac catgtgcgtg cgcggcttcc cataatggct gtgcttcgct
     9661 gaataagtca tcattccaca ttaaatggcc ctgactaaag ttttcagggt tcagcacagg
     9721 atcaattgcg agaaaatttt ttgcctgata ataactaacc cacgactcag ggtaattggt
     9781 gtagaaagcc actttaggtc gcgtgaatgg taccgggtgg cgaacacata acgaatagta
     9841 atcgtactcc agctgctgag cctgaagctc tatttcatgg taggcctctt ctgcggcctc
     9901 catcctctga aaacgcaaca gcatcgtgcg acgccagctg aaaaaatccg tatcctgcat
     9961 agtaaaccgc aacgcccctg agagtgagta tcatttataa tgataattca aactatcaca
    10021 taaataagat ttatatataa tttatattat tcaggcaatg aattactttt gcaagccatc
    10081 gcattctctt atgttattaa tgagttatgc tgatttgtta agcagtttta tcacgcttga
    10141 aatggcgtcc cgccccgaca ggtgaatcgt cggggctgat tttttcttat tattgcagca
    10201 gaagcttctc gaggaactgg cgggtgcgag gctgctgggg atcggcaaat aacgcttttg
    10261 ctgccccctg ctcgactatc cgcccctggt ccataaagat cgcccggtcc gcaacatccc
    10321 gggcaaagct catttcgtgt gtcacaatca ccatcgtgcg cttttcctgc gccagctggc
    10381 ggatggtgtt caggacttcg cccaccagct ctggatccag cgccgacgtt ggctcgtcaa
    10441 acaaaatcac ctcaggacgc attgccagtg cacgcgcaat cgcaacacgc tgctgttgac
    10501 cgccagacaa acgacgtgga tagctggttt ctttaccggc cagcccaact ttcgccagca
    10561 actcgcgagc gcgcgccgtg gcctcttctt tcggttcgcc tttgacgatc accggccctt
    10621 caataatgtt ttccagcacc gtacgatgcg gaaacaaatt aaagttctgg aagacaaacc
    10681 cgacgtgctg acgcaactgg cgaatcagcg atttttgctg acttaatgaa cgtgcagtat
    10741 caatagtgat atcgccaacg gtgatcgttc ccgcttccgg ttgttccagc agatttatgc
    10801 tacgtagcaa tgtggttttg ccggaaccac tcggaccgat aattgccacc acttcgccag
    10861 gctttacctc gaggtcgata ccgtgcagca ccgtctgacc gtggaatttt ttcaccaggt
    10921 tcttaacttc aatggcactc attttggttc tctctcctgg cgattaagtt gattctcaaa
    10981 atggttctgc aacgtcgata acacagtcgc catgatccag tagatgagcg aagccgccag
    11041 gtacatggtg aacacctcca gcgtgcgcga ggtaatcaac tgcgcctgac ggaacagctc
    11101 cggcacctgg attgtcgcgg ccagcgacgt atcttttacc agactgataa agctgttcga
    11161 cagcggcggc aacgccaccc gcgccgcctg tggcaaaatc gcgcgacgca tggtctgcca
    11221 cggcgtcata ccaatactgg cagcggcttc ccactgacct ttatcaatag aagaaatggc
    11281 ggcgcgcagc gtttcggcgg cataggcggc agtgttcagc gacagaccaa tcattgctga
    11341 cggaatggga tctaattcga taccaaactg cggcaggccg taatagatca taaacagttg
    11401 tgcgatgagc ggcgtaccac gaaaaataga gatataaaaa cgcgccagcc agcgcacggg
    11461 ccagattggc gacaggcgca tcagcgcgag aataaacccg agtagtaagc caaaaaacat
    11521 gccgccgata ctgagttgca gggtataccc agcccctttc aacaggaacg gcaaggaatc
    11581 aataaccagt tgtatacttt cttgcatgag cgttcccgga gctaaacgtg gggatgatag
    11641 gcgaacagcg caggcgcgcc gccggtatga ataaacagaa tcggcccttc atctttgaag
    11701 cgtttctgac tgataccgtc aatcagcccc gccatcgctt ttccggtata cacaggatca
    11761 agcagaatgc cttccagacg cgccagcaat ttcactgctt ccatgccttc gtcgttcggc
    11821 acgccgtagc caggtgcaaa atagtcatcc cagagtaaaa tttccgctga tgcggtcagc
    11881 tccagttctt tcgcaatcgc ctgttgtagg ttaaccactt tcggcaattg atcggcaacg
    11941 gaacgcgaca cggtcacgcc aatcagttcg ctttcaggca tcaggtgttc cagcccaaca
    12001 gccagtccgg cgtgagttcc ggcactgccc gatgcgacta ccaccgacga aatattaacc
    12061 gccccttcac actgttgcgc gatttccagc gcactctcca cataacctag cgcgccaaga
    12121 gcattagaac cgccaaccgg aatgacatac gggcgaaagc cttgtgcttc gactcgcgtc
    12181 gccagttctt ccagttgggc attgggatcg gtcagtgcgt cgcacatttc aatctgggta
    12241 ttgaacagat ccagcaacaa acgattgccg ttggttaaat agttttctgc ggttgtgcca
    12301 ataggatttt ccagcagcgc cacgcagtgc agaccgagtt tcgccgcgac tgcggcagtc
    12361 tggcgcacat ggttagactg gatcgcgccg gcagtaatca gcgtatcggc accttcgcgc
    12421 agggcatctg ccgcgagaaa ttccagctta cgtaatttat tgccgcccat tgccatgggg
    12481 gtgacctcat cccgtttgat gaaaatttcc cgtccaagat aatcagaaaa gcgcggcaga
    12541 tattcgagcg gcgttggcgc gccgataaac tccaaccgtg gaaaacgggt taaattatgc
    12601 agtggcataa cagcctccga tgtgtgttgt tgtgattttc ttattatgca cgctgaaaac
    12661 gcgtaaataa aaaaggcgct agtgaaagcg cccttttttg tcattatgct gattatttgg
    12721 tcacatcagc accaaaccat ttttcggaaa gagcttgcag agtgccgtct ttttgcattt
    12781 ccgcaattgc atcattcacc gctttcagca ggtcttcatt tcctttacgc agcgccacgc
    12841 cagactcctg acgggagaat gcttcaccgg ttactgccag cgtatcgttg gttttcttca
    12901 ccagatccag cgccgccaga cgatcaacga ggatcgcatc gatacgccct acgcgcagat
    12961 cctgatattt ggtcgggtca tcatcatagg tacgcacatc gacaccctga acattctgcc
    13021 gcagccactc ttcatagttg gtgcccagac cgacacccac ttttttgcct ttcagatcag
    13081 cagccgtttt aatggtgcct tcgttacctt ttttcaccag cgcctgaata ccggaaatgg
    13141 tgtaaggggt agagaaatcg tattttttct tgcgctcgtc agaaatggtg acctgattaa
    13201 tcaccacatc aatgcgttta gagtccagcg acgccagcat accgtcccat ttggtcggtt
    13261 ttagtgacgc ctcaacgcca agatgttttg ccagctgttg ggcaaattcc acttcaaaac
    13321 cggttaattt gccgtcatcc ccctgaaaac tgaacggcgg ataagtgcct tccagcccta
    13381 ccagcagcgt gccgctctct ttgactttat taagcagacc ttcatctgca aaacttttaa
    13441 cgctcatgcc cgcaaccagc gccacggcca tcacacccat caatgcctga cgtcccagat
    13501 gtgctaattt catattcacc ccgaatgttg ttatgtctgt ttgcagtgta gagccattgt
    13561 ttgtaaacac aaaaacaact ccgctacatc ttattcttat ttaatatata tcagaagcag
    13621 gcaggctgga ggatttctgc acaagtccgg tacaggtttg gcgctgatag tgttgatact
    13681 tccgtaatgc gatgcggtaa ttgttggtgc tggtggtggg cagccacggg gcgaggattt
    13741 catcaaggaa accgtcctgc agctgatcga gggaaatatt ttgctcatgt agatgattac
    13801 ccaaacggcg cagacgaacg acatattcac gcaccgtacc tgggctcatt tcagtttgct
    13861 caaaaagaaa tttcttaaag ccgataatat cgaaaaaatc actctgcgtt ttgcaatgta
    13921 aatcaccgca aaatcggcac aatgccgccc atgattgttg ttccgtttgc cagccttttt
    13981 catcaagcag cgtgtccagg cgggaaatct cgattttatt cactattttg ccgtcgcgaa
    14041 ctaaggtaat gcgatcgagt aatttacggc aatgcgcgca atgggtctgg ctgtgtttaa
    14101 agtctttaag atagcggctt aatggccgtc ttttcaggtg ctgcaccatc attaagaact
    14161 cctggtagtc aaagttaaag tgcggcattt actgacgtta taacttaccc agtttagtgc
    14221 gtaaccgttt aatggcctgg ctgtgtaact gactgacccg cgattccccg acctccagca
    14281 ccgcgccaat ctctttgaga ttcagctctt cctggtaata gagggttaat accagttttt
    14341 cgcgctccgg caacgtttcg atggcttcca tcacccgctg gcgcagatta ctgtccagca
    14401 gttgttgtag cgggttttct cgctgatgat catcagtaac cagttcgatg ctatcgccgt
    14461 gctcttcacg ccactcatcg taggagaaga gctggctgtt attggtgtcg agcagcattt
    14521 gacgataatc ggcaatatcg atccctaaac gttccgctac ctcagtttcc gtggcgttgc
    14581 ggccaagttc ctgctccagt tgccctattg cctgtgccac ttcacgcgcg ttgcgtcgca
    14641 cgctgcgcgg cacccagtca cggctgcgaa gttcatccag catagcgcca cggatacgct
    14701 acactgcgta agttgtaaat gccgttcctt gtagggcgtc atagcgttcg acagcattaa
    14761 gtaacccaat gccgcccgcc tgtagcagat cgtcaagttc cacgctcgcg ggcagtcgaa
    14821 cctgcaggcg caatgcttcg tgacgcacca gcgggacata acgctgccac agcgagtgtt
    14881 tatccattac accttcagcg gtatagagtg aattcacgat aaacagccct gcgttatatg
    14941 agttatcggc atgattatcc gtttctgcag ggtttttaat cggacgatta gtgggtgaaa
    15001 tgaggggtta tttgggggtt acgcaaaaag ccgaatgtaa aaaaccccgc cggtggcggg
    15061 gtttgagagg cattcagacg attaaccctg cagcagagac agaacctgct gcggtacctg
    15121 gttggctttt gccagcacgg agttaccggc ctgctggata atctgcgctt tcgacatgtt
    15181 ggacacttcg gtcgcatagt cggcgtcctg aatacgggac tgcgcttcag acaggttggt
    15241 agtggtgttg ttcaggttgg tgactgcgga atccaaacgg ttttgcaccg cacccaggga
    15301 ggagcggaat ttgtcgatgg agctgatggc ttcatccaga gctttcaggg gatcggctgt
    15361 cgtagctgct tttgtttcag catctgtcgt caatttacca tcagcatctt tataaactgc
    15421 cttaccgctg ccgttagtca ctgaaccatc atcctgtagg taaaaatcta cagtatcaga
    15481 tgtggtcaac gcctcgttgt tatcaacata aaccgaatca acaccttttg ttacagcgcc
    15541 agttgtgccg ttgacgctat aacctgcagt agtgaaatca acattgtttc ccgaggttga
    15601 tacagcttta tcgaaagctg cctgatcaac ttttgcacct gttactgaat aagtaatagt
    15661 gtccggcgta gtactattac cggtaaagga gatagaggta ccgccaatgt caatggttgt
    15721 cgctttggta ttattggctg caagtgtgga cagactagcc tcagtcaaag aagcaccaga
    15781 gccgtttttc gttaagcgcc cagttgtatc aatgtagagt ttatctccat tgcttgccgt
    15841 aattttacca tcactgctga tctgaacatc ctgtgcagaa ccatcgatag taatagtgcc
    15901 cttagcggta tcaccaactc ccggggtcaa atatttctga acatcggcag ctgaagccgt
    15961 tgtggtatca aaagagtatg acttacttgc actgtcatac ttgtaattcg ttgcactagc
    16021 cgccgttccg aagccgttat taatggttgc ctgaacagta tcaccatcac taactccagc
    16081 cagcaaatca gacgctttag cagcatcgta acccgcactc acagtatatt tgttgccgac
    16141 cactgttgca ttagcagcta ccaagtcagc tttagatgca gcagtattag ccacagcccc
    16201 gccaccattc acattaaacc cattcagccc cagcgtatca gagtcaattt tcttcagatc
    16261 aatagtgata gtctggccgt cattcgcacc aacctgaatt ttcatcgaac cgtcttttgc
    16321 cagtacgttc acgccgttga actgggtctg accggatacg cggtcaattt cgtcgagacg
    16381 ggatttgatt tcgtcctgaa tggagtccag atccgaatca gagttagtcc cggtagaagc
    16441 ctgaaccgtc agctcacgga tacgctgtaa gttgttgttg atttcggaca gcgcgccttc
    16501 agtggtctgt gcaacagaaa taccgtcgtt ggcgttacgt gcagcctgag tcaggccttt
    16561 aatgttagaa gtaaaacggt tagcaatcgc ctgacccgcg gcgtcatcct tcgcgctgtt
    16621 aatacgcaag ccagaagaca gacgctcgat agaactcgac agcgcagact ggttcttgtt
    16681 gatattattt tgagtgatca gcgagaggct gttggtatta atgacttgtg ccatgattcg
    16741 ttatcctata ttgcaagtcg ttgattacgt tttgggtttc cacccgtcgg ctcaatcgcc
    16801 gtcaaccctg ttatcgtctg tcgtaaaaca acctttagaa tttttttcac aaacagccat
    16861 tttttgttag tcgacgaaat actcttttct ctgcccctta ttcccgctat taaaaaaaac
    16921 aattaaacgt aaactttgcg caattcaggc cgataacccc ggtattcgtt ttacgtgtcg
    16981 aaagataaaa ggaaatcgca tggcaagtat ttcatcgctg ggagtcgggt caggtctgga
    17041 tttaagttca atccttgata gcctcaccgc cgcgcaaaaa gcgacgctaa cccctatttc
    17101 aaatcagcaa tcgtcgttta ccgctaaact tagcgcctac ggtacgctga aaagcgcgct
    17161 gacgactttc cagaccgcca atactgcatt gtctaaagcc gatctttttt ccgccaccag
    17221 caccaccagc agcaccaccg cgttcagtgc caccaccgcg ggtaacgcca tcgccgggaa
    17281 atacaccatc agcgtcaccc atctggcgca ggcgcaaacc ctgaccacgc gcaccaccag
    17341 agatgatacg aaaacggcga tcgccaccag cgacagcaaa ctcaccattc aacaaggcga
    17401 cgacaaagat ccgattacca ttgatatcag cgcggctaac tcatcgttaa gcgggatccg
    17461 tgatgccatc aacaacgcaa aagcgggcgt aagtgcgagc atcattaacg tgggtaacgg
    17521 tgaatatcgt ctgtcagtca catcaaatga caccggcctt gataatgcga tgacactctc
    17581 ggtcagcggt gatgatgcgc tacaaagttt tatgggctat gacgccagtg ccagcagcaa
    17641 cggtatggag gtctcggtcg ccgcccagaa tgcgcagctg acggtcaaca acgtcgccat
    17701 cgagaacagc agcaacacta tcagcgacgc gctggaaaac atcactctga acctgaacga
    17761 tgtcaccacg ggcaaccaga cgctaaccat cactcaggac acctccaaag tgcaaacggc
    17821 gattaaagac tgggtgaatg cctataactc gctaatagat accttcagca gcctgaccaa
    17881 atacaccgcc gtagatgcgg gagctgatag ccagagttct agcaatggcg cactgctcgg
    17941 cgactccacg ctgcggacga ttcagacgca gttgaagtcg atgctgagta ataccgtcag
    18001 ttcttccagc tataaaacgc tggcgcagat tggtatcacg accgatccca gcgatggcaa
    18061 actggaactg gatgccgaca aactcaccgc tgcactgaaa aaagatgcca gcggcgtagg
    18121 tgcattgatt gttggcgatg gtaaaaaaac cggcatcaca accaccatcg gcagcaacct
    18181 gaccagttgg ctttcgacaa cgggcattat taaagccgct accgatggcg ttagtaaaac
    18241 cctgaataag ttaactaaag actacaacgc cgccagcgat cgcattgatg cgcaggtcgc
    18301 gcgctacaaa gaacaattta cccaactgga cgttttaatg acctcgttaa acagcaccag
    18361 cagctactta acgcagcagt tcgaaaacaa cagtaattcc aagtaatcat tattcatcgg
    18421 gagacaggtc atgtacgcgg caaaaggcac ccaggcctat gcacaaattg gcgtcgaaag
    18481 caccgtaatg agcgccagcc aacagcagtt ggtcaccatg ctatttgatg gagtgctgag
    18541 cgcactggtt agagcgcgac tgtttatgca ggacaacaat cagcaaggca aaggcgtctc
    18601 tttgtcaaaa gcgatcaaca tcattgagaa cggactgcgg gtgagtcttg atgaagagag
    18661 caaagacgaa ttaacccaaa acttaattgc tctttatagc tatatggtca ggcgtttgct
    18721 gcaagccaat ttacgcaacg atgtctcagc agtcgaagag gtggaagcat taatgcgtaa
    18781 tattgccgat gcctggaaag agtctttact ctccccttct tcgattcagg acccagtctg
    18841 atgaacaatg caccgcattt gtatttcgcc tggcaacaac tcgtcgaaaa aagccagctc
    18901 atgttacgcc tggcaacgga agaacaatgg gacgaactca tcgctagcga aatggcgtat
    18961 gtgaatgcgg tgcaggagat cgcacacctg actgaagagg ttgccccgtc cactacgatg
    19021 caggagcagc tccgcccgat gctgcacctg attctcgaca acgaaagcaa ggtaaagcag
    19081 ttattacaga ttcggatgga tgaactggcg aaactggtcg gtcagtcatc ggtgcaaaaa
    19141 tcggtgttaa gtgcctatgg cgatcagggc ggctttgtgc tggctccgca ggataacctc
    19201 ttttgaatct taatgagccg atggctcgcg aataatccga ttacggctac gcttctaata
    19261 ttccccttga atggagtcga agaatgcgta atcccacgct gttacaatgt tttcactggt
    19321 attacccgga aggcggtaag ctctggcctg aactggccga gcgcgcagac ggttttaatg
    19381 atattggtat caatatggtc tggttgccgc ccgcctataa aggcgcatcg ggcggctatt
    19441 cggtcggcta cgactcctat gatttatttg atctaggtga gtttgatcag aaaggcagca
    19501 tccctactaa atatggtgat aaagcacaac tgctggccgc cattgacgcc ctgaaacgta
    19561 atgacattgc ggtgctgctg gacgtggtag tcaaccacaa aatgggcgcg gatgaaaaag
    19621 aagctattcg cgtgcagcgt gtaaatgctg atgaccgtac gcaaattgac gaagaaatca
    19681 ttgagtgtga aggctggacg cgttacacct tccccgctcg tgccgggcaa tactcgcagt
    19741 ttatctggga tttcaaatgt tttagcggta tcgaccatat cgaacaccct gatgaagatg
    19801 gcatttttaa aattgttaac gactataccg gagaaggctg gaacgatcag gttgatgatg
    19861 aactcggtaa tttcgattat ctgatgggtg agaatatcga ttttcgcaat catgccgtga
    19921 cggaagagat taaatactgg gcgcgctggg tgatggaaca aacgcaatgc gacggctttc
    19981 gtcttgatgc ggtcaaacat attccggcat ggttttataa agagtggatc gaacacgtac
    20041 aggaagttgc gccaaagccg ctgtttattg tggctgaatg ctggtcgcat gaagtcgata
    20101 aactgcaaac gtatattgat caggtggaag gcaaaaccat gctgtttgat gcgccgctgc
    20161 agatgaaatt ccatgaagca tcgcgcatgg gtcgcgacta cgacatgacg cagatcttca
    20221 ctgggacgct ggtggaagcc gatccttttc acgccgtaac cctcgttgcc aaccacgaca
    20281 cccaaccgtt gcaagccctc gaagcaccgg tcgaaccgtg gtttaaaccg ctggcgtatg
    20341 ccttaatttt gttgcgggaa aatggcgttc cttcggtgtt ctatccggac ctctacggtg
    20401 cacattacga agatgtcggt ggtgacgggc aaacctatcc gatagatatg ccaataatcg
    20461 aacagcttga tgagttaatt ctcgcccgtc agcgtttcgc ccacggtgta cagacattat
    20521 ttttcgacca tccgaactgc attgccttta gccgcagtgg caccgacgaa tatcccggct
    20581 gcgtggtggt catgtcgaac ggagatgatg gcgaaaaaac cattcatctg ggagagaatt
    20641 acggcaataa aacctggcgt gattttctgg ggaaccggca agagagcgta gtgacagacg
    20701 aaaacggcga agcgaccttc ttttgcaacg gcggcagcgt cagcgtgtgg gttatcgaag
    20761 aggtgattta aatccatccc cggcggcaag ccggggagat ttcattacgg cagtggcgtc
    20821 ggtaacggcg ctttatccag cgcggcggca cactctgccg tcgggcgatc cacacgctcc
    20881 atcgtcatac catcgtactc aatggtgtta ccgtcacgct caacctcgta cagctcacgt
    20941 tttacggtaa cgttagtgag atcatcagac atcaatgtca acttacccgg cacggcaatc
    21001 ccccgctgcc actgacggca atccaaggta tcgccctctt tagtgacaat caggctgcct
    21061 atcgcttccg gactgaccag cgcacgttgc ggccctttgg tctgccagta gcccgccagc
    21121 cagtccggcg cgggggtttt cacgacattg ttatagtttt cgacttcggc acagcccgcc
    21181 agcaacatga gcgcgcctgc aattgcgagt tttttcatca ttcgtcctgc atgagaagaa
    21241 aagaggattg tggcattaaa gctctgatgt cgccagcctt tccaggttca gagagaaatt
    21301 tgatctaact attatttttg tgaattatta ccccgaaaat aaatttgcat ggtgtatgat
    21361 tcgcggctct tgtttcaaac cttctgagtt cagaggctac attcatgtca tggcagcaat
    21421 tcaaacacgc ctggttgatt aaattctggg cacccatccc tgcggtcatc gcggcgggta
    21481 ttctctccac ttactatttt ggcattactg gcaccttttg ggctgtcacg ggtgaattta
    21541 cccgttgggg cggtcagctc ctgcagctgt ttggcgtcca tgctgaagag tggggttatt
    21601 ttaaaattat ccatctggaa ggatcgccat taacccggat cgacggcatg atgatcctcg
    21661 gtatgtttgg cggctgcttt gctgctgcgc tgtgggccaa caatgtcaaa ctacgcatgc
    21721 cacgcagccg tatccgcatt atgcaggcca tcattggcgg cattatcgcc ggttttggcg
    21781 cgcgtctggc aatgggctgt aacctggcgg cgttctttac cggcattcct cagttctcgc
    21841 tgcatgcctg gttctttgcc atcgccactg ccattggttc atggtttggc gcgcgcttta
    21901 ccctgctgcc catcttccgt attcccgtga aaatgcagaa agtttctgcc gcctcaccgc
    21961 tgacgcaaaa accggatcag gcgcggcgtc gttttcgtct cgggatgctg gtctttttcg
    22021 gcatgctggg ctgggcgctg ctcacggcga tgaaccaacc aaaactgggg ctggcaatgc
    22081 tgtttggcgt cggctttggt ttactgattg aacgtgcgca aatctgcttt acttcggcgt
    22141 tccgcgatat gtggatcacc ggacgtaccc atatggcgaa agcaatcatt atcggtatgg
    22201 cggtgagtgc catcgggatc ttcagttacg tacagttagg cgttgaacct aaaatcatgt
    22261 gggcgggacc aaatgcggta attggtggtt tactttttgg ttttggcatc gtgctggcag
    22321 gcggctgcga aaccggctgg atgtaccgcg cggtagaagg ccaggtgcac tactggtggg
    22381 tcggtctggg caacgtgatc ggctcaacga ttctggcgta ttactgggat gatttcgctc
    22441 cggcgctggc caccgactgg gacaaaatca acctgctgaa aacttttggt ccgatgggag
    22501 gcctgctggt gacatatttg ctgctgtttg ctgcgctgat gttgattatc ggctgggaaa
    22561 aacgcttctt ccgccgtgag gcaccgcaga ctgctaagga gatcgcatga aaaatatcgt
    22621 tcctgattac cgccttgata tggtgggtga accctgccct tatccggcag tcgcaaccct
    22681 tgaggcgatg ccgcaattaa aaaaaggtga gatcctggaa gtggtgagcg actgtccgca
    22741 gtcgatcaat aatattccac tggatgcgcg taatcacggc tacacggtgc tggatattca
    22801 gcaagacggg ccgaccattc gttatttaat tcagaagtaa tccctcattc ccgctgtggc
    22861 ccacagcggg aacatcgtta cctctgacac atagatttta cccgccccct ctcctattct
    22921 tttcagtatg atctcaggag gttgctatgt gtggacgctt tgcccaatcc caaacgcgtg
    22981 aggattacct tgcgcttctc gcggaagata ttgaacgcga tattccctac gatcccgaac
    23041 ccattggcag atacaatgtc gcgccgggaa ccaaagttct tctgctcagt gaacgtgatg
    23101 aacaccttca tctggatccg gttttctggg gatatgctcc cggatggtgg gataaaccgc
    23161 cgctgattaa cgcccgcgta gaaactgcct caaccagtcg catgtttaaa ccgctctggc
    23221 aacatggtcg ggcaatctgt tttgccgatg gctggtttga gtggaaaaaa gaaggcgaca
    23281 aaaaaagcct tattttatct atcgcgctga cggacaacct gtttttatgg ccgcgatagg
    23341 tagcataccg tttgagcgtg gtgacgaagc cgaaggattt ttgatcgtca ctgcggcggc
    23401 agatcaaggt ctggtagata ttcatgaccg ccgcccgctg gttctgtcgc cagaagctgc
    23461 acgggaatgg atgcggcaag acattggtgg taaagaagcc tcagaaatcg ctaccagaag
    23521 ctgtgtgcca gcaaaccagt tcacctggca ccctgtgtcg cgcacggtgg gtaatgtcaa
    23581 aaaccagggc gcggagttaa ttcaacctgt ttgttgacat cctccacgcc ctgaatgacg
    23641 aggacccctg ctacgttcag gctgtcgcct gaatcatttc ggtgggttcc tgcttcaacg
    23701 agcggcctga ctgcaccatc tctccacagg caagaacggc gtgccccgcc gctaaaatgt
    23761 tacgagctcc atttacatcg gcgttcgctg tatatccaca tacctggcat ctgaatttac
    23821 tttgtgacag gcgattttct ttcgctgtat gaccacagca cgcgcaacgc tggcttgtgt
    23881 acgctggcgg aacagcaagc acctgaccgc cacgccagag ctgcttgtac tcaagctggc
    23941 ggcgcatttc ataccagccc tgatccagta tcgaacggtt taaacctgat tttgcccgga
    24001 cattgcgccc cggctggctg accgtacccg ctgctgactt tgacatgttg ctgaccttca
    24061 aatcctcaat gactatcatt acgtggtttt tgctgacggt cgttgtgact ttgtgaaggt
    24121 agtccctgcg gatatttgcg atacaggaat gcagtcgctg tattttgcgt ttctgcttct
    24181 gccagttgtt gctgaatttg accttgcggc ttaactgtcg ctgaagtctc gccagcgtct
    24241 tctggttttt ctgaaaactg tttacaggct caaagactgt gccatctgac agcgaggcaa
    24301 gtttagccac gccagcatcc agcccgacca ttgatgctga agggtgaacc ggagttgata
    24361 cttcactttc tgcctgaata ctgatgtacc acttaccgca ggactggctg acagtgacat
    24421 ttttcacaac acccgtgacc tgacggctgt tccggtagcg catccagcca agttttggca
    24481 gaaaaatacg gctgttttcc tgagcgagct taacgccctg cggatagcgg aatgcatcat
    24541 tctgtcaccg ctttttgaat caggggaacg ccgagcgctt ctggaaaaag tttttgcagg
    24601 cctgctcagg gtctttcagt gactgttgca atggctgtga cggagcatct ttaatccatt
    24661 gcgtttcagt ggcatttttc cactcaacca atcaggaagc cattttcgcg taagggatgt
    24721 ctttgttacc ggccttatga ttctcattct gacgtgccag agtacgactg aaaacgaaac
    24781 gacatgcgcc tgcgaagcgc ctcatttcac gcgcctgttg accaccgggt cttaaccgga
    24841 atttgaatac ctgtagtcgt ttcacagggc aaattatact ttggtctgtg aaaaaaactg
    24901 atatccgacg cggaaggcac tgtgttttcc tgatgcatgc tcaccgggtc tttgttacca
    24961 agtaccgacg ccagattttt gaccatgacg cgacagaaaa actacgcact tacttttcaa
    25021 atgtatgtgc ttattttgaa gctgaactgg ttgaaatgga tggcgaacca gatcacgtcc
    25081 atctgttaat taactatccc cccaaactgg cgatatccag tctggtaaac aaacagcctc
    25141 aaaggcgtat cgggtaggtt actgcgacgg gatcgaccag atattgctgc caggtattac
    25201 cgcaaagatg ttttgtggcg tcccggctat tttgccagta gctgcggagg tgcgccaata
    25261 tccgtcatcc gccaatacat tgaacaacag caaacacctg gtcagatgaa aaaacgagcc
    25321 ttatatcccc gccctgaagg acgggctttt acggtgcact ggataagcct ggagattatc
    25381 tacacctgca tggacatcat tgtacgatgc cccgtacatg ctccatcaca tcctcaacat
    25441 caaagtatta gagccttgca aaccattatg cgtctcagtt tgtctcatct atcttacgca
    25501 aaaaaaatag aactaatatc aagcggcgct atattgcgta ttaaataaaa ccacctcaat
    25561 tacattaata aatctaaata aatatcatgc aaataatttc catggttatt tttaggctat
    25621 ttaaattaaa aaaataaatt atgagtcaat cataaataac aacctttaat ataatttgac
    25681 taccaaattt atattcgttc acaaaatagt catgcattgt gtgcaataga aacagttcag
    25741 ataaaaatag ggatctactt cacaatttaa atgagaaact aaaacttaca tcttgaaata
    25801 atctcattga ttggatgaat atttgtagtg cagtgacatc atttttaaat aatagttcaa
    25861 ataaaagggc tcacgatgaa aaaattaaca gtggcaattt ctgctgtagc tgcatcagta
    25921 ctgatggcga tgtctgctca ggcagcagag atttacaata aagacagtaa caagctggat
    25981 ctgtacggaa aagttaatgc taagcactac ttctcttcta acgatgcaga tgatggcgac
    26041 acaacttatg ttcgtctggg cttcaaaggc gaaactcaaa tcaacgatca actgactggt
    26101 ttcggtcagt gggaatatga attcaaaggc aaccgtgctg aatctcaagg ttcttccaaa
    26161 gataaaaccc gtcttgcctt cgctggcctg aaattcggtg actacggctc catcgattat
    26221 ggccgtaact acggtgtagc atacgatatc ggtgcatgga ccgacgttct gccagaattc
    26281 ggtggcgata cctggaccca aacagatgtg ttcatgactg gtcgcaccac aggtgttgca
    26341 acctatcgta acaacgactt ctttggtctg gttgatggtc tgaactttgc tgctcagtat
    26401 cagggtaaaa atgaccgcac tgacgtaact gaagctaacg gtgatggttt cggtttctcc
    26461 actacttatg agtatgaagg attcggtgta ggtgcaacct atgctaaatc tgaccgcact
    26521 aatgatcagg ttatctacgg taacaacagc ctgaatgcat ctggtcaaaa tgctgaagta
    26581 tgggcagctg gtctgaaata tgatgcgaac aacatctatc tggctaccac ctattctgaa
    26641 acccagaaca tgactgtttt tggtaataac catattgcca acaaagcaca aaacttcgaa
    26701 gtagttgcac aatatcagtt cgacttcggt ctgcgtccgt ccgttgctta cctgcaatct
    26761 aaaggaaaag acttgggtgc gtggggtgat caggacctga ttgaatatat tgatgtaggt
    26821 gcaacctatt acttcaacaa aaatatgtcc acttttgttg attacaaaat caacctgatt
    26881 gataagagcg atttcacgaa agcatctggc gttgctaccg atgatatcgt tgctgtagga
    26941 ttggtttacc agttctaatc tgattactca aagatatgtt gtgggaggct ttgcctcccc
    27001 aacatataag tggcgctctc aagccacttt ccttagaagc actatcttgc ttctgactat
    27061 ataaattacc ctttattttc tgggacgttt caacgcccca tttttaataa tactcagtga
    27121 acaattaacg tatcaattag atttaataac aacgatatcc gtacctaacc tgatatctaa
    27181 tgtcataagc atccctttaa ttatcccctc aacctcttgt aaatttccca ctcttgagct
    27241 ccacatagcc caaacgccgg atgcaaaaaa aatcatacgt gcatctctga aattgtgatg
    27301 gaggaagtga gtatcaatta tatgtgctta taaacgttcc cgattggcac agaagtcacc
    27361 ggattgttca ggctccaata ggttgagtat gtcgtattta ttttccaaaa taaaaatcga
    27421 tgctgttgct ccttaataaa atacttttac tctctggacg cttttcttag ctctcttggt
    27481 gttatattaa aacgattatg gaacctttca gtaaaacgag aaggacactt gtaaccattt
    27541 tccctggcaa tctcgctgat gggttttact gtcgtttgta tagcaggaca acgcattatt
    27601 taacctcacg tcgtccaata tactttgaaa acttattccc tcgcttgcca gacggcgatg
    27661 caatgtggaa acagaaatgt agagatatct cgctaccttg tttgctgtcc attttgttct
    27721 aggttcggat agcattaggt tataacaacg acttattaat gattgtttac tatctgataa
    27781 aagtaaacaa ttaacatgat gtgctcctaa tgaaagtaga acgcccattg ctaagtgttc
    27841 ctgaatttgc gttgagaagc ctcgggaaat agatgttttt agttgctccc aacaatatat
    27901 taactcgggg ttctgaggta agcagaaacc tgtcgtgtta cgaatttgat cagttatcgc
    27961 ataaaatttt tgaaactttt caattagatc aatgggataa taaagcattt ctgcaagata
    28021 aagacctgct tctggataat tcacaatata aaattcatat ccacagggaa ataatattat
    28081 ctgattatta tcaacagtta gactatgcgt ctcccaattg ataacttttt ttccctgacg
    28141 gatacgacat aaagctggca taagaggctt aaccctgtga atctcatgat gtttatgcat
    28201 ccgtatttct tcaatcttta ggttagactt atctcttgcc agcatactct taccctattt
    28261 tatctcataa actggtgtta tctcagcggt tgcaatttta ttggcattaa gcatataacc
    28321 aactaacgct ccgctggaat tagaatccac aggaatctta tcagttttta gagcccatac
    28381 tttaaactgg taatgatggg gtttatctcc tttgggagga catgctccac caaatcctgc
    28441 ataaccaaaa tcatttcggc cttgaacagc accagtcgga agttttgtac catcacgtct
    28501 tcctgcatca gtgggcaaat atgttacagt tgctggaata ttagcaacag tccagtgcca
    28561 ccatccactg cctgtaggag catctggatc atacacagtt acggcaaagc ttttggtgcc
    28621 ttcaggagca ccagaccagg ttaatgaggg cgatgtatta ccaccttcac acccaaatcc
    28681 agaaaataca tgagacgttg taagttgctc tcctgttttt atttcattac tagtgacctg
    28741 aaatgctgcg gcctgcgcag aaaatgttat gaatgccaat acacttgaaa cgataagttt
    28801 tttcataaaa acctcattgt tgtgacctat cgttatttta tttgatgtgc cttgatctca
    28861 ttatgcataa aggcgcaatg atcgcgcaaa tgcaatcata attataaagg tgccccgaac
    28921 attagccata atatgcacaa atcagattcc tgttatctca aggagaatta ttttttaatg
    28981 cggtgcactt cgtgacaaca agttaatgac taacacaccg gcacaaatca acatcatgcc
    29041 tatgatagct ggcaggtcca gtcgttgacc aaaaaatccc caggaaagca aactaatcag
    29101 aacgataccg actcctgacc agatagcata agcaatccct gtaggtatat aagccagcgt
    29161 ctgagctaat aaccagaatg atgcacaata gcaaataatt gtgccaacag agggccataa
    29221 ccgtgtaaac ccctctgaaa acttcattaa ggttgtacca atgacctctg caagtattgc
    29281 gccaccaaga taaatataag ggttcatagc atattctttc ctgttcatac tggagagaat
    29341 tgtactacag tttgaacaca actcaccgtt ttcatcctgg tgtatgtgta tccaaggggc
    29401 tctggcgtgg caagagataa aggcaggaac aataatgatc agaaggaacc ccaggctaac
    29461 gaatgagttc actcttcctg ggagcaaagc gaagacaaaa catgttatcg cgtttacgga
    29521 tcgtggatgg cagaaaacaa ccaggatcag gtatccattt taaaccagaa attgagtgag
    29581 tttgccccat ccatgcccca cgctgtggga tcggatataa ttaaacaagc atagtcaaca
    29641 ggttacacaa cctacacctg catggacatc acttcctgat acgccgccac cagcttatta
    29701 cgcacctgaa tccccatttg catagaaact gaggcttttt gcatatcggt catcacatcg
    29761 tttagcgcca cgccgggttc accgagggtg aatttttctg cctgcgtgcg ggcgactgtt
    29821 tgtgtatcgc ttatgcgatc gagcgcggcg tgcagctgcc cggcaaaact aatggtcggt
    29881 tgcggcagtg attcctgcgc acgcgcactc atcgccgtag cctgtaactg gctgataacc
    29941 ccttcaatcc cctgtatcgc tgacattttc gtctcccgga taatttctgg taacaaagcc
    30001 taccagtaag tcaataagac aaaggcgcta aatagcaaca aaaaaactgg tttattggcg
    30061 gatagaaaaa acgaaagcac aaataatggg agcgtcaatt tttcgagtgt gctgacccga
    30121 gagtgagtct tgttccactt tgccaataac gccgtccata atcagccacg aagtgcgcga
    30181 tgaatgcgac tgcagcccag acaaaatctc ttgagtggct taatcgcctg cgtgcgaatc
    30241 cgaaaattcc attgattgtt accggttccg cggcagtggc ggtcatggtc gcactgatcc
    30301 tgtgggcgaa agcccccgac taccgcacat tattcagcaa tctttccgat caggatggtg
    30361 gcgcaattgt cagccaactg acgcaaatga atattcctta ccgcttcagc gaagccagcg
    30421 gcgctattga ggttccggca gataaagttc acgaactgcg cctggcgcaa caaggtttgc
    30481 ccaaaggcgg cgcggtcggt ttcgaactgc tcgatcagga aaagtttggt atcagccagt
    30541 tcagcgaaca ggtgaattat cagcgggcgc tggaaggcga gctttctcgt accatcgaaa
    30601 ctatcggccc ggtaaaaggg gcgcgcgtac atctggcaat gccgaaaccg tctttattcg
    30661 tccgtgaaca aaaatcccct tctgcatcgg tgacggtaaa tctgttaccc ggccgcgcac
    30721 tcgatgaagg gcaaattagc gctattgtac atctggtttc cagcgcggtt gctggtctgc
    30781 cgcctggaaa cgtcacgctg gtggatcagg gcgggcatct gttaacccag tccaatacca
    30841 gcgggcgcga tcttaatgac gctcagttga aatatgccag cgatgtcgaa ggccgtattc
    30901 agcagcgtat tgaagcgatc ctgtcaccta ttgttggtaa cggtaatatt cacgcctagg
    30961 tcacggcgca actggacttc gccagtaaag aacaaacgga agaacagtat cgccctaacg
    31021 gtgatgaatc tcatgcggcg cttcgttcac gccagcttaa tgagagcgag caaagcggtt
    31081 ccggttatcc gggcggcgta ccgggggcgt tgtcgaatca accggcacct gcgaataacg
    31141 cgccaatcag cacgcctccg acaaatcaaa ataaccgcca gcagcaggcg agcaccacca
    31201 gcaatagtgg gccgcgtagc acacagcgga atgaaaccag taactacgaa gtcgatcgca
    31261 ccattcgtca taccaaaatg aacgtgggcg atgtgcaacg tctgtcagtc gcggtagtgg
    31321 tgaattacaa aaccttgcca gacggtaaac cattgcctct cagcaacgaa cagatgaagc
    31381 aaattgaagc tctgacccgc gaggcgatgg gcttttctga aaaacgcggt gattcgctca
    31441 atgttgttaa ctcgccgttc aatagcagtg acgaaagcgg cggagcactg ccgttttggc
    31501 aacagcaagt gtttatcgat cagttgctgg ctgccggtcg ctggttgctg gttgctggta
    31561 ctgctggtgg cgtggttgct gtggcggaaa gcggtacgcc cgcagctaac acgtcgcgcc
    31621 gaggcggtga agactgtgca gcaacaggcg caggcccgcg aggaagtgga agatgcggtg
    31681 gaagtccgtc tgagcaaaga cgaacaactc caacaacggc gcgctaacca acgactgggg
    31741 gcagaagtca tgagccagcg tatccgtgaa atgtctgata acgatccgcg cgtggtggcg
    31801 ctggtcattc gccagtggat aaataacgat catgagtaac ctgacaggca ccgataaaag
    31861 cgtcatcctg ctgatgacca ttggcgaaga ccgggcggca gaggtgttca agcacctctc
    31921 ccagcgcgaa gtgcaaaccc tgagcgctgc aatggcgaac gtcacgcaga tctccaacaa
    31981 gcagctaacc gatgtgctgg cggagtttga gcaagaagct gaacagtttg ctgcactgaa
    32041 tatcaacgcc aacgattatc tgcgttcggt attggtcaaa gctctgggtg aagaacgtgc
    32101 cgccagcctg ctggaagata ttctcgaaac ccgcgatacc gccagcggta ttgaaacgct
    32161 caactttatg gagccgcaga gcgccgccga tctgattcgc gatgagcatc cgcaaattat
    32221 cgccaccatt ctggtgcatc tgaggcgcgc ccaggccgcc gatattctgg cgttgttcga
    32281 tgaacgtctg cgccacgacg tgatgttgcg tatcgccacc tttggcggcg tgcagccagc
    32341 cgcgctggcc gaactgaccg aagtactgaa tggcttgctc gacggtcaga atctcaagcg
    32401 cagcaaaatg ggcggcgtga gaacggcagc cgaaattatc aacctgatga aaacgcagca
    32461 ggaagaagcc gttattaccg ccgtgcgtga attcgacggc gagctggcgc agaaaatcat
    32521 cgacgagatg ttcctgttcg agaatctggt ggatgtcgat gatcgcagca ttcagcgtct
    32581 gttgcaggaa gtggattccg aatcgctgct gatcgcgctg aaaggggccg agcagccact
    32641 gcgcgagaaa ttcctgcgca atatgtcgca gcgtgccgcc gatattctgc gcgacgatct
    32701 cgccaagcgt ggtccggtgc gtctgtcgca ggtcgaaaac gaacagaaag cgattctgct
    32761 gattgtgcgc cgtcttgccg aaactggcga gatggtgatt ggcagcggcg aggataccta
    32821 tgtctgataa tctgccgtgg aaaacctgga cgccggacga tctcgcgcca ccaccggcag
    32881 agtttgtgcc aatggtcgag tcggaagaaa ccattattga agaggccgaa cccagccttg
    32941 agcagcaact ggcgcaactg caaatgcagg cccacgagca gggttatcag gcggggattg
    33001 ccgaaggtcg ccagcaaggt cacgaacagg gctatcagga aggactggcc caggggctgg
    33061 aacaaggtct ggcagaggcg aaggctcaac aagcgccaat tcatgcccgg atgcagcaac
    33121 tggtcagcga atttcaaact acccttgatg cgcttgatag tgtgatagct tcgcgcctga
    33181 tgcagatggc gctggaggca gcacgtcagg tgatcggtca gacaccaacg gtggataact
    33241 cggcactgat caaacagatc caacagttgt tgcagcaaga accgttattc agcgggaaac
    33301 cacagctgcg cgtacacccg gatgatctgc aacgtgtgga tgatatgctc ggcgcaactt
    33361 taagtttgca tggctggcgc ttgcggggcg atcccaccct ccaccctggc ggatgtaaag
    33421 tctccgccga tgaaggcgat ctcgacgcca gtgtcgccac tcgctggcaa gaactctgcc
    33481 gtctggcagc accaggagtg gtgtaatgac cacgcgcctg actcgctggc taaccacgct
    33541 ggataacttt gaagccaaaa tggcgcagtt gcctgcggta cgtcgctacg ggcgattaac
    33601 ccgcgctacc gggctggtgc tggaagccac cggattacaa ttgccgctcg gcgcaacctg
    33661 tgtcattgag cgccagaacg gcagcgaaac gcacgaagta gaaagcgaag tcgttggctt
    33721 taacggtcaa cggctgtttt taatgccgct ggaggaagtg gaaggtgtcc tgcccggcgc
    33781 gcgtgtatat gccaaaaaca tttcggcaga agggctgcaa agcggcaagc agttgccgct
    33841 cggtccggcg ttattaggtc gtgttctgga cggtagcggt aaaccgctcg atggcctgcc
    33901 cttccccgat acgacggaaa ccggtgcgct gattaccccg ccatttaacc cgttgcaacg
    33961 tacaccgatt gaacatgtgc tggacaccgg cgtgcgccca atcaatgccc tgcttaccgt
    34021 cggacgcggg cagcgtatgg ggctgtttgc cgggtcgggc gttggtaaaa gtgtgctgct
    34081 ggggatgatg gcacgctaca cccgcgccga tgtcattgtc gtgggtttga ttggtgaacg
    34141 tgggcgcgaa gtaaaagatt ttattgagaa tatcctcggt gccgaaggac gtgcacgctc
    34201 agtggtgatt gccgctccgg cggatgtttc tccgctcctg cgaatgcaag gtgccgccta
    34261 tgccacgcgc attgccgaag attttcgcga tcgtggtcag catgtgttgc tgattatgga
    34321 ctccctcacc cgctacgcga tggcccagcg tgaaattgcg ctggcgattg gcgaaccacc
    34381 tgccactaaa ggttatccac cgtcggtgtt tgccaaatta ccggcactgg tcgagcgtgc
    34441 cggtaatggc attagcggcg gcggctcgat taccgcgttt tataccgtgc tcaccgaagg
    34501 cgatgaccag caggacccca ttgctgactc cgcgcgggca atcctcgacg gtcacattgt
    34561 gctgtctcgc cgactggcgg aagccgggca ctatccggct atcgatattg aagcgtcgat
    34621 cagtcgcgca atgacggcgt tgatcagtga gcaacattac gcgcgagtgc gcaccttcaa
    34681 acagctgttg tcgagttttc agcgtaaccg cgatctggtt agcgtcggcg cgtatgccaa
    34741 aggcagcgat ccgatgctcg ataaagccat cgccctgtgg ccgcagctgg agggctattt
    34801 gcaacaaggc atttttgaac gcgcggactg ggaagcgtct ctccaggggc tggagcgtat
    34861 tttcccgaca gtgtcataac ccaggagata acggcagatg gcggaagaac agctcaaaat
    34921 gttgatcgat tatcagaatg aatatcgcaa taacctcaat agcgatatga gtgccgggat
    34981 gaccagcaac cgctggatca actatcagca gtttatccag acgctggaaa aagccattac
    35041 tcagcatcgc cagcaactta atcagtggac gcagaaagtt gacattgccc tgaacagctg
    35101 gcgagaaaag aaacaacgtt tgcaggcctg gcagacactg caggaacggc aatccacggc
    35161 ggcactgctt gcagaaaacc gcctcgatca gaaaaagatg gatgagttcg cccagcgcgc
    35221 cgccatgagg aaacctgaat gattcgctta gcgcccttga ttaccgccga cgttgacacc
    35281 accacattgc ctggcggcaa agccagcgat gctgcacaag attttctcac gttgttgagc
    35341 gaagcattag caggcgagac aacaactgac aaagcggccc cccagttgct ggtagcaaca
    35401 gataagccca cgacaaaagg cgagccgctg gtcagcgata ttcttgccga tgcgcaacaa
    35461 gcggatttac tgatccctgt ggatgaaaca cttcctgtca tcaacgacga acaatccaca
    35521 tcaacaccgt taaccaccgc tcagacgatg acgttggctg cggtggctga caaaaatacg
    35581 acaaaagacg aaaaagcgga tgatcttaat gaagacgtca ccgcaagtct gagcgccctt
    35641 tttgcgatgt tgccgggttt tgacaatacg cccaaagtga ctgatgcacc gtcaaccgtg
    35701 ttaccggcag agaaaccaac gctcttcaca aaactgactt ctgcgcaact cactacagca
    35761 cagcctgatg acgcccccgg cacaccagct cagccattaa caccgctggt agcagaagcc
    35821 cagagtaaag cggaagtcat cagcacgcct tcgccggtga ccgctgacgc cagcccgcta
    35881 atcactccac accagacaca gccactgccc accgtcgccg cgcctgtttt gagtgcaccg
    35941 ctgggttctc acgaatggca acaatcattg agccagcata tttcgctgtt cacccgccag
    36001 gggcaacaaa gtgcagagtt gcgtctgcac ccacaggatt taggtgaagt gcaaatttcc
    36061 ctcaaagtgg atgataacca ggcgcagatc cagatgattt caccgcatca gcacgtacgc
    36121 gccgccctgg aagcagcgct gccggtactg cgtacgcagc tggccgaaag tggcattcag
    36181 ttagggcaaa gcaacattag tggcgaaagc tttagtggtc agcagcaggc cgcttcccag
    36241 caacagcaaa gccaacgcac agcaaaccat gaacctctgg cgggggaaga cgacgatacg
    36301 cttccggttc ccgtctcttt acaagggcgt gtaacaggta acagcggcgt tgatattttc
    36361 gcctaacgtc agaggtagca ccgtaatccg cgtcttttcc ccgctttgtt gcgctcaaga
    36421 cgcaggataa ttagccgata agcagtagcg acacaggaag accgcaacac atgactgatt
    36481 acgcgataag caagaaaagc aagcgatcgc tttggatccc gattctggta ttcattaccc
    36541 tcgcggcctg tgccagcgca ggttacagct actggcattc gcatcaggtt gccgctgacg
    36601 acaaagcgca gcaacgcgtc gtgccctccc cggtcttcta tgcgctggat acctttacgg
    36661 tcaatttggg cgatgcggat cgcgtgcttt atatcggcat aaccctgcga ctgaaagatg
    36721 aagcaacccg ctcgcggctg agtgagtatt tgccggaagt ccgtagtcgc ttgctgttac
    36781 tgttttcacg tcaggatgct gccgtactgg cgacagaaga aggcaagaaa aacctgattg
    36841 ccgagattaa aaccacactt tccaccccgc ttgttgccgg gcaaccgaaa caggatgtca
    36901 ccgacgtgct gtataccgct tttattctgc gataacgaca tgggcgatag tattctttct
    36961 caagctgaaa ttgatgcgct gttgaatggt gacagcgaag tcaaagacga accgacagcc
    37021 agtattagcg gcgaaagtga cattcgtccg tacgatccga atacccaacg acgggttgtg
    37081 cgcgaacgtt tgcaggcgct ggaaatcatt aatgagcgct ttgcccgcca ttttcgtatg
    37141 gggctgttca acctgctgcg ccgtagcccg gacattaccg tcggggccat ccgcattcag
    37201 ccgtaccatg aatttgcccg caacctgccg gtgccgacca acctgaacct aatccacctg
    37261 aaaccgctac gcggcacagg gctggtagtg ttctcgccga gtctggtgtt tatcgccgtg
    37321 gataacctgt ttggcggcga tggacgcttc ccgaccaaag tggaaggtcg cgagtttacc
    37381 catactgaac agcgcgtcat caaccgtatg ttgaagctgg cgctggaagg ctatagcgac
    37441 gcctggaaag cgattaatcc gctggaagtt gagtacgtgc gttcggaaat gcaggtggaa
    37501 tttaccaata tcaccacctc gccgaacgac attgtggtta acacgccgtt ccatgtggag
    37561 attggcaacc tgaccggcga atttaatatc tgcctgccat tcagcatgat cgagccgcta
    37621 cgggaattgt tggttaaccc gccgctggaa aactcgcgta atgaagatca gaactggcgc
    37681 gataacctgg tgcgccaggt gcagcattca cagctggaac tggtggccaa ctttgccgat
    37741 atctcgctac gcctgtcgca gattttaaaa ctgaaacccg gcgacgtcct gccgatagaa
    37801 aaacccgatc gcatcatcgc ccatgttgac ggcgtcccgg ttctgaccag tcagtacggt
    37861 actctcaacg gtcagtatgc gttacggata gaacatttga ttaacccgat tttaaattct
    37921 ctgaacgagg aacagcccaa atgagtgaca tgaataatcc ggccgatgac aacaacggcg
    37981 caatggacga tctgtgggct gaagcgttga gcgagcaaaa atcaaccagc gaaaaaagcg
    38041 ctgccgacgc ggtgttccag caatttggcg gtggtgatgt cagcggaacg ttgcaggata
    38101 tcgacctgat tatggatatt ccggtcaagc tgaccgtcga gctgggccgt acgcggatga
    38161 ccatcaaaga gctgttgcgt ctgacgcaag ggtcagtcgt ggcgctggac ggtctggcgg
    38221 gcgaaccgct ggatattctg atcaacggtt atttaatcgc ccagggcgaa gtggtggttg
    38281 ttgccgataa atatggcgtg cggatcaccg atatcattac cccgtccgag cggatgcgcc
    38341 gcctgagccg ttagtgatga ataaccatgc tactgtgcaa tcttccgcgc cggtttctgc
    38401 tgagccactg ctgcaggtga gcggcgcact catcgccatt attgccctga tcctcgctgc
    38461 tgcctggctg gtaaaacggc tgggatttgc ccctaaacgc actggcgtta acggtctgaa
    38521 aattagcgcc agtgcttcgc tgggcgcgcg tgaacgggtc gtggtggtcg atgtggaaga
    38581 tgcacggctg gtgctcggcg ttaccgcagg gcaaatcaat ctgctgcata aacttccccc
    38641 ttcagcacca acggaagaga taccgcagac cgattttcag tcggtcatga aaaatttgct
    38701 taagcgtggc gggagatcct gatgcgtcgt ttgttttctg tcgcaccggt ccttctctgg
    38761 ctgattacgc ccctcgcctt cgcgcaactg ccgggtatca ccagccagcc gctacctggc
    38821 ggtggacaaa gctggtcgct cccggtgcag acgctggtgt tcatcacctc gttgacgttc
    38881 attccagcga ttttactgat gatgaccagt ttcacccgca tcattattgt ttttggttta
    38941 ttgcgtaacg cgctgggaac gccctccgcg ccacctaacc aggtattgct ggggctggca
    39001 ctgtttttga ccttttttat tatgtcgcca gtgatcgaca aaatttatgt cgatgcgtac
    39061 cagccattca gcgaagagaa aatctcaatg caggaggcgc tggaaaaagg ggcgcagccg
    39121 ctgcgtgagt ttatgctgcg tcagacccgc gaggcagatt tagggttgtt tgccagactg
    39181 gcgaataccg gcccgttgca ggggcctgaa gctgtgccga tgcgcatttt gctcccggcc
    39241 tacgtgacca gcgagttgaa aaccgcattt cagataggct tcacgatttt catccctttt
    39301 ttgattatcg acctggtgat tgccagcgtg ttgatggcat tggggatgat gatggttccc
    39361 cccgccacca ttgctctgcc ctttaaactg atgctgtttg tactggtgga tggctggcaa
    39421 ttgctggtcg gttcgctggc gcagagcttt tacagctaga gaggcaaaat gacacctgaa
    39481 tcggtcatga tgatgggaac tgaagcgatg aaagtcgcgc tggcactggc agccccgctg
    39541 ttgctggtag cgctggtcac gggccttatc atcagtattt tgcaggcagc cacgcagatt
    39601 aacgaaatga cgctgtcgtt tattccgaaa atcatcgccg tatttatcgc catcattatt
    39661 gccggaccgt ggatgctcaa tctgttgctg gattacgtcc gcaccttgtt cactaacctg
    39721 ccgtatatca tcgggtagcc cgtacaatga tgcaggagac aagcgatcaa tggctttcct
    39781 ggttaagcct gtacttctgg ccgttactgc gcgtgctggc gctcatttct accgcgccaa
    39841 ttctgagcga acgcagcgtg ccgaaacggg taaaactggg tctggcaatg atgatcacgt
    39901 tcgccattgc cccatcatta cctgccaacg atgttcctgt tttttcgttc tttgctctgt
    39961 ggctggccgt acagcagatc ctgatcggca ttgcgcttgg ttttaccatg caatttgcct
    40021 ttgccgctgt gcgaaccgct ggcgaaatta tcggtctgca aatggggctg tcatttgcga
    40081 catttgtcga tcctggcagc catctcaata tgcccgtttt agcgcgcatc atggatatgc
    40141 tggcattact gctgttcctg acatttaacg gtcatttatg gttgatttca ctgctggtcg
    40201 atacctttca caccctgccg attggtagcg aaccgttgaa cagcaatgcg tttctggcac
    40261 ccaccaaagc agggagtttg attttcctta acgggctgat gctggcgtta ccgctcatta
    40321 ctctgttgct gaccctgaat ctggcattag gtttactgaa tcgtatggcc ccgcaattat
    40381 ccatttttgt tattggattt ccattaactc tgactgtcgg catctcttta atggcggcat
    40441 taatgccgtt aattgcgcct ttttgcgaac atttattcag tgaaattttt aatttgctgg
    40501 ctgatattat tagtgaatta ccattaatat aattccgtaa cgtttatcat gctaccctaa
    40561 ggattatccg aaaaataata tctgcgaaca tcttccagga tactcctgta gcgaaatatt
    40621 tgttttaagc tcactcacat atcgcaacat ttactttaag acaattccag gcaaattata
    40681 caacacttta cgggatagta agtccgcctg aaaaattgcg agagtggcgc attaggtgac
    40741 ccatgttgtt ccgtttagtc atgatgaaat attcaggtaa ggggaattat cgttacgcat
    40801 tgagtgaggg tatgccatgt caacgattat tatggattta tgtagttaca cccgactagg
    40861 tttaaccggg tatctgttga gtagaggggt taaaaaaaga gaaatcaacg acattgaaac
    40921 cgttgatgac cttgccatag cttgtgattc acagcgtcct tcagtggtgt ttattaatga
    40981 ggactgtttc atccacgatg cttctaacag tcagcgcatc aagcacatca ttaatcaaca
    41041 tcccaatacg ttatttatcg tttttatggc aattgccaat gttcattttg atgaatatct
    41101 attggtcaga aaaaatttat tgatcagttc taaatcgatt aaaccggaat ctctcgacga
    41161 tatccttggc gatattctga aaaaagagac aacgataacc tcgtttttaa atatgccgac
    41221 gttatcattg agccgaaccg aatcgagtat gttgcgaatg tggatggcag gtcagggaac
    41281 cattcaaatc tccgaccaaa tgaatatcaa agccaagacc gtttcatcgc ataaaggtaa
    41341 tattaaacgt aagatcaaaa cgcataataa acaggttatc taccatgtcg tccgactgac
    41401 ggataatgtg actaatggta tttttgtcaa catgcgctaa cacattctga ctggtggttt
    41461 cccaccagtc aggctgaata agattactct gctttctcaa caaagatacc gtcctgatgc
    41521 cctgcttcat taaagaacca gatgccgagc gggtagtctt ccagcgcaac caggtacatt
    41581 gtgccttcac taaactcctc aactgccagt accactccag gacgacgcgg accgccatcc
    41641 gttttgactg ttacccgatc attcaccttc atcgttttcc tcctgtggct ttgtgccagt
    41701 gtagaacaat ttcgtttttc tggcagcgcc aagcgcgcgc gagtgctgat tttctcgacg
    41761 gtctatactt aagagatgcc aggcggactt aacgactggc ggcaacaaca gagtaacggt
    41821 tgcgaggaaa gatgatgaaa accgcaaaag tgtacagcga taccgcaaaa cgtgaggtca
    41881 gcgtcgatgt cgatgccctg ctggcggcga tcaatgaaat tagcgaaagc gaagttcatc
    41941 gcagccagaa cgattctgaa cacgttagcg tcgatggacg tgaatatcat acatggcgtg
    42001 aattggcgga tgccttcgaa ctggatattc atgacttcag cgtctctgaa gtgaatcgtt
    42061 gaatgcacaa taaaaaaatc ccgaccctga gggtgtcggg atgaaacttg cttaagcaag
    42121 aagcacttaa aaaattcgtt acaccaggaa atctgatgtg ttcatcacct tatctgcaat
    42181 ttttttcgct gacaagaaaa tattcgcgct atgaatgatt aacgctactt atgaatagaa
    42241 atgtgacgcg cgtcacccct tggtggctct ggtgtcaacg ggatcgctcc cgaaaaaatc
    42301 atctttgatt atttgtgctg ttttgagaat catcctggta aggggagtac tgcgcgacca
    42361 tgttttcaat tcaacaacca ctactggttt ttagcgatct tgatggcacc ctgctggaca
    42421 gtcatagtta tgactggcaa ccggcagccc cctggctcag ccgtttacac gaagccaata
    42481 ttcccgtcat tctctgtagc agtaaaactt tagcggaaat gctgtacttg caaaaaatgt
    42541 tggggctaca aggcttaccg ctgattgcag agaatggcgc agtgatccag cttgctgaac
    42601 aatggcagga tatagacggt tttccacgca tcatctcagg tattagccat ggcgaaatct
    42661 gccaggtttt aaatacgcta cgcgagaaag aacattttaa attcacgact tttgatgatg
    42721 tcgacgatgc aaccattgcc gaatggacgg gattaagccg tagctaggcg gcgctgacgc
    42781 agctgcatga ggcgtcggta acgctaatct ggcgcgacag tgacgagcgt atggcacaat
    42841 ttatcgctcg tctgaacgaa ctgggcttgc agtttatgca gggtgcgcgc ttctggcacg
    42901 tactggatgc ctctgccgga aaagatcagg ctgccaactg gattatcgcg acctatcaac
    42961 aattgtcagg cagacgccca accacacttg gcctgggcga tgggccaaac gatgcgcctt
    43021 tactggaggt aatggattac gcggtgattg tgaaagggct aaaccgtgaa ggggtacatc
    43081 tgcatgatga ggatccggcc cgcgtctggc gaacgcagcg tgaaggaccg gaaggctggc
    43141 gtgaagggct ggaccatttt ttctccgccc gttaagcgtt atcgctcgcg aatacccgat
    43201 tgcgcccggc ctgttttgcg aggtaaagcc gacggtcggc caatgactgg agttgttcaa
    43261 aatcataatc accggtttcc tcgctgctac ttacccccag cgaggcactg atgcgtatcg
    43321 ttgtactctt ggcgatcagc atctcttttt catttaactt caggcgaata cgttctgcga
    43381 cctccgcagc ctgcgtcaga ttcgcgcctg gcagaatcac acaaaactcc tcaccaccga
    43441 cccgcccggc aacgtcctgc gcacgcaagg aactgctaat taatccggca gcatgagaaa
    43501 gaacacggtc gcccgcctga tgaccaaagc ggtcattaat cgctttaaaa tggtcaagat
    43561 cgacctgaat gacagaaaaa ggatgttggt gcgtctgaca caatttagcg agcggacggg
    43621 ctttttcgaa cagtgcgcca cggttatata aacgcgttaa ggtgtcgtgc cacgcctgcc
    43681 actgcaacga gctttgcaga acatacatgt tgctgaccat ccggcgaatc acataccagg
    43741 agaggagtaa catggtggta aagagcgccc acagcagggt taatgcaatg ctgatactgc
    43801 cgaaatcgcc gcgcacgcct tcgcttagcg tatggacgcg cgccagcaca ccgtcgaaat
    43861 ggtccagacg ttcccagcta acatagcgac tgtccatacg aatgccgccc cgcgtgtcat
    43921 gttccattgc ctgcgccagc aaggccagtt cacgaggatc aaaaatattc cctgttggat
    43981 ggtcaggatt ggaagaggtc aaaaatctca gcttactgtc atagagctga tactcaccat
    44041 cgaggttttt atcgatggcg tttcttaaaa attgctgcat agtacgcacg ggaatactca
    44101 tccccagcac gccataccag tagttattac tgtctaccgg aacactgacg gtaacctgcg
    44161 gttcagtatt gctggcgtgt tccggttgcg aagtgaacca gcgtaccgcg cggtgacgat
    44221 tttctcgttg cgaatggccg ataaaccagg gttgggtgac atagccgtaa taacgcgttg
    44281 gtacattgcg cgtaaacaag gtcggctgcg tcgaaacgta aaatccagca cgcgagacat
    44341 acatcgcctg ttcaaccatc gacgaggagt tgtgcgccag tcgcagcaag taaccaactt
    44401 ccagtgcagc ggtaatttca ttgtcgaggc tttcattttc gcgagacagg aaattcccct
    44461 cgctgactaa tgcatccgat acgccattga ctggcagggt gcgccgtcgg ttgagttcga
    44521 tttgccaggc gtgctcgtcg cgatgctgtt cgaactcggt tacggcatta cgcagagagg
    44581 tgaaatccag tggcgccacg agagcttcgc gcatgccatt acgcaaaaag atcagtttgt
    44641 cgacgttata ctgcaaatgc ttatcgagcg cgttggctac gttttccaga tgattacgct
    44701 agctggagat ataggcatct tccagcacca ccacttcacg ccaggtgagc aaggtggaaa
    44761 aaagcaatac cacgataaag cagagattaa cgacatgacc gggccccagg cggcgtgcga
    44821 gttttttcaa ccagctctgg ttttccattt ttgtctcgtg ctgcaccctg acgactccca
    44881 acctgctttt ttatgattct ggcataaccc ggcaatagcg taccgcaaaa cagcgataat
    44941 tttataatgg tggcatgata agacgcgact atcattcatt gcaggcattg ggacaccagg
    45001 ccggatgtga tccggcctgg tgttaatgat accctttcaa acttcttccg cagaacgact
    45061 cccctccccc ggaacaagat cggtttcatg aaaagcttcc cgcttgaccc cgtaaccgtc
    45121 ataccaacga cactcaacca taccgctgga gtatccagtg acaatcatcc gcgggccgcc
    45181 ctctttaacc gtaacttcct cactaaccat aaagctcata ctcgcctcct tttttcgagt
    45241 gaaacttgtt caccttagtt gaagatggcg aattttgcat aatagccagt gcgagtatta
    45301 atgtgcctga ccgcgcattt tcgctactgc tttcactccc agcaccacga tgccgccgat
    45361 gataaatcca agaatcagat ttaaaacagt cggtaatatc atcgccacca ctgcactttg
    45421 ctgcccggcg atatgttcaa tagcgtgatg cagcggcgca ataccatgta ccacaatccc
    45481 gccgccgacg aggaacatcg ccagcgtgcc gacaatcgat aatgctttca tcagccaagg
    45541 cgcgataatc aataaccctt tacctaatgc ctgcatcagc gcgctagatt tttccgccag
    45601 ccaataccct aggtcatcaa tcttaacgat aacccctacc agaccgtaga cgcccacggt
    45661 cactaccagc gcgatgcctg aaagcaccag cacctgatta agcaacggcg cctctgccac
    45721 aatccccagc gtgatggcga cgatttccgc agacaaaata aaatcggtac gaatcgcccc
    45781 tttaattttg tccttttcaa acttcagcgg atcctgcgcc gccagcttct ccagacgctg
    45841 ctggctctgc gctggatctt ctttatgttt acgcgcctcc agcatatgca gcactttctc
    45901 taccccttca aagcagagaa acgcgccacc aatcatcaac agtggcgtaa tcgcccacgg
    45961 gataaacgca ctgatgatca gcgccagcgg caccagaatc actttattga tcagcgatcc
    46021 tttcgccacg ccccagacca cgggaagttc ccggttggcc cgcacgcctg aaacttgttg
    46081 cgcattgagc gataaatcat cccctaatac accggcggtt ttcttcgccg ccagtttgcc
    46141 catcacagag atatcgtcca gcagtgtggc gatatcatcg agcaacgtca gtaaactact
    46201 cccggccaaa agcattccct ctcattttta ttgttgaagg gtgtaagtat gaagcaaaaa
    46261 cggcagctct gatacttgct tcacaggtca acatttattt aacaatcaag tgcaattata
    46321 tctctcgcca gcagaatgat tttcacgttt actatgaacc acctttttat ttccgccgtg
    46381 agggattatg cgtttccgcc agttgttacc gctttttggc gcgctgtttg cgttgtatat
    46441 catttggggc tcaacctatt ttgtcattcg gattggcgtg ggaagctggc ctccgttaat
    46501 gatggcgggc gttcgattcc aggcagccgg tattttattg ctggcatttt tgctactgcg
    46561 cggacacaaa cttcccccgc tacgtccgct gctcaatgcc gcgctgattg gcctgttatt
    46621 gctggctgtc ggtaatggca tggtgacggt tgccgaacat caaaatgttc cttccggcat
    46681 cgccgccgta gtgattgcaa ccgtgcccct ctttaccctg tgcttcagtc gcctgtttgg
    46741 cattaaaacg cgcaaactgg aatgggtggg tattgccatt gggcttgccg gaatcatcat
    46801 gctcaatagc ggtggaaatt taagcggcaa tccgtggggc gcgattctga ttttaatcgg
    46861 ctcgattagc tgggcgtttg gctcagttta tggctcgcgc attaccttac ctgtagggat
    46921 gatggcgggt gcgattgaga tgctggcggc aggcgtggtg ttaatgatcg catcgatgat
    46981 tgcgggtgaa aaactgacgg cgctcccttc cctgtcaggc ttccttgcgg tcggctatct
    47041 ggcgctgttt ggttcgatta tcgccattaa cgcttatatg tatttaatcc gtaatgtcag
    47101 tccggctctc gccaccagct acgcctacgt taacccggtg gtcgcggtct tgctgggtac
    47161 gggactgggt ggcgaaacgc tgtcgaagat tgaatggctg gcgctcggcg taattgtttt
    47221 cgcggtggta ctggtcacgt tgggaaaata tctcttcccg gccaaacccg ttgtcgcgcc
    47281 agttattcag gatgcatcaa gcgagtaaat gaatcccctg cgtgtcgatc tgcgcgctgg
    47341 caccttcgcc gcagatccac tcttccagac gctcggtaag cgccgcatcc gtcagcttct
    47401 cgcgcctacg taacgcacac tcccagacaa tcagtacgcg ccagccgagc tcctgcaagc
    47461 gactgatatc gcggcgatcg cgttcaacat ttttacctat cttctccagc cagaactcag
    47521 ttcgagtcgc aggcacttta aacagatagc agtgatgatg atgccagaag cagccgtggg
    47581 taaatatcac gcagcgatat tcatcaacga caaaatccgg gcgtccgggc agactggcgt
    47641 cctgaacgcg aaacgcaagg ccctgcccgg ttaacaaact ggcgaggcgc ttctcaatcg
    47701 ccgtatcacg cgtggcaatc gcgcgcatat ttttgctgcg ggtggcctta tcgtgaacgt
    47761 cgaccatgtt gtgcctcttg ctgacgcaac gccaccgcct gtttgatttt tggctcaagc
    47821 agttttgcca ccgcggcaaa gaccggcacg accaccgagt tgccgaactg gcgataggcc
    47881 tgagtgtccg aaaccggaat gcggaatttc gcttctcccg gcgcttcaaa acccattaag
    47941 cgcgcgcatt cacgaggcgt taaccgacgt ggacgatgtt gctgattcag cggatcgtca
    48001 aagtctttct cacccgtggc catatcccaa ccgcgatcga ttaaaatttc cgcgccatct
    48061 ttgtaataac gagcagacag cgtacgggtg acgctttgcg gattgttcgg ataaaccatt
    48121 ccataaccga agccgttacc gcgcgcctga tgttttttcg catatcgata gaggtacttc
    48181 cacagcaccg gcgtcaggat atatttcgcc tcaaccatcg ggtccagcag ctgcgccagc
    48241 gtcactcgct gtgcagggaa acattcgctg atatcacgca gagtaaaatc ggctttcaga
    48301 ttcagatcgc gacgaaaacc caccagcacg atgcgttcac ggtgctgcgg caggaaatgt
    48361 ttaccatcga tgattttcgg atcgtccggc ccgttatctt ctgcatcggc aacgtcatag
    48421 cccagttcgt ccagcgtctg catgatgatg cggaacgttt ttcccttatc gtgactcttc
    48481 aggtttttga cgttttcgag cacaaacatc gccggacgac gcgcgtcgat aatgcgcacc
    48541 acatcaaaga acagcgtgcc ctgggtatcg caggcaaaac cgtgcgcccg cccgagcgag
    48601 ttctttttcg atacgccagc cagcgaaaat ggctgacaag ggaaaccggc cagtaaaaca
    48661 tcgtgttcag gaatgtgttg acgaatatgt tccgccgccg cctcatcact cacgccttct
    48721 tgatgactga gggtgatgtc gcggatatct tcattaaaat gatgcgtcgc cgggtcgcaa
    48781 taatggttgg ctttataagt gcgtaccgca tgtttgttcc attcgctggt aaacacgcac
    48841 tgtccaccaa tcgattcaaa accacgacgg atgccgccaa ttccggcaaa tagatcgata
    48901 aagcgaaacg cataatgcgg atgatgttcc ggtggtttgg gtaacaacgt ttgcagatgg
    48961 gcaaactctt tctcacttaa acggtgccat gccgagtcat tcgccagcgc acgttttaaa
    49021 attgccgcgc tccagtgatt ctcacccaca ccattaagct gcgccaccag cattttaaca
    49081 tcataaattt gcagcagttt ctccagcatt gcctgtgcgg cattcccggt gctgtatgaa
    49141 tcggttactg atatattttc ctgcatagat ttaaccggcc atctgagatg gccgacaggt
    49201 taacataatt actgccgttt agggccacgc gctcagccag cgggtgaaaa cgcatctatc
    49261 accttgtgat cgataccttc attctccccc gccagttcgg caccgagctt cgccatcaaa
    49321 cagcgccaag ctcaatgccc ctgaaacggc aaatacgcgc gccaggccaa tcgcccccag
    49381 ggcttccagc cgatccgcat cctggacgat tttagcttcc gttgttaagg gggcgatttg
    49441 cgcgctgaaa ctgtgagcgg caatggcatg acaaacggcc tcaattttct ccgccggaaa
    49501 ttgcacgaac tcttcacgga gcaggcgacg cgtctcttct gccgccagga ttgaagaacg
    49561 ctgccgttgc ggatgatttt tcgccaggct cacgatatcg tgaaaataac aggcggttaa
    49621 aatcaccagc atatcgacat cgtcatctgc cgccagtttc tgtgccgtag cccagacacg
    49681 gcgaaaatga cacaggtcat gcgccgcgtc ctgatgttga tgatggtttt tcaaccagtt
    49741 ctcgaactat gcctgccagt gttgtaactc catacgcgct ccgttgccaa agagtctcaa
    49801 ccttagcagt ttttattcat agcgtctgca acgtgcggga cgaggtcggg tgtaccatgc
    49861 aatgccgcct aacaaggcac caaccagggc cataaacaga aatccgacca atgctcctaa
    49921 ccacttcccc gcggtagatc ctaaatcact ttcaaatccc gacacggggg catcggggat
    49981 ccggctcatc acccagatgt atcgcgccat tgcccagacg aaatagccac accatgtata
    50041 gaaagcccac
//
LOCUS       DAEBXN010000019        49830 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.019, whole genome shotgun sequence.
ACCESSION   DAEBXN010000019 DAEBXN010000000
VERSION     DAEBXN010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49830
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.019"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..1224
                     /locus_tag="KJL99_001086"
     CDS             <1..1224
                     /locus_tag="KJL99_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HBD4435891.1"
                     /translation="FELPEITDNELLVSVISDSVCLSTWKAALLGSEHKRVPDDLENH
                     PVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSGYSAGYSYEYFGGNATYMI
                     IPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYHTTQYVYEHRMGVKPGGNI
                     ALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQVQKLLPVDLAASKGIELVY
                     VNTKGMSDPIQMLRALTGDVGFDDIFVYAAVPAVVEMADELLAEDGCLNFFAGLTDKN
                     FKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQLQPSFMVTHIGGLDAVPET
                     VLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKELARLVEETHGIWNEQAEK
                     YLLAQFGVDIGEAAQ"
     gene            1221..2186
                     /gene="yggF"
                     /locus_tag="KJL99_001087"
     CDS             1221..2186
                     /gene="yggF"
                     /locus_tag="KJL99_001087"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HBD4435892.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            2208..2717
                     /gene="fumE"
                     /locus_tag="KJL99_001088"
     CDS             2208..2717
                     /gene="fumE"
                     /locus_tag="KJL99_001088"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HBD4435893.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            2714..3427
                     /locus_tag="KJL99_001089"
     CDS             2714..3427
                     /locus_tag="KJL99_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HBD4435894.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKLLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQALPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPAAALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRGLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(3399..4076)
                     /locus_tag="KJL99_001090"
     CDS             complement(3399..4076)
                     /locus_tag="KJL99_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HBD4435895.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHNAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(4070..4747)
                     /locus_tag="KJL99_001091"
     CDS             complement(4070..4747)
                     /locus_tag="KJL99_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBD4435896.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPREITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVIIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(4735..5118)
                     /locus_tag="KJL99_001092"
     CDS             complement(4735..5118)
                     /locus_tag="KJL99_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBD4435897.1"
                     /translation="MQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRA
                     RGVPLDEGWYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADS
                     TLQRVARYTMILLMLTEFGAWIWLR"
     gene            complement(5134..5442)
                     /locus_tag="KJL99_001093"
     CDS             complement(5134..5442)
                     /locus_tag="KJL99_001093"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4435898.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLLILAIVSTSQL
                     "
     gene            complement(5443..6021)
                     /locus_tag="KJL99_001094"
     CDS             complement(5443..6021)
                     /locus_tag="KJL99_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBD4435899.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVSGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(6044..6475)
                     /locus_tag="KJL99_001095"
                     /pseudo
     CDS             complement(6044..6475)
                     /locus_tag="KJL99_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            6847..7866
                     /gene="epd"
                     /locus_tag="KJL99_001096"
     CDS             6847..7866
                     /gene="epd"
                     /locus_tag="KJL99_001096"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HBD4435900.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7916..9079
                     /gene="pgk"
                     /locus_tag="KJL99_001097"
     CDS             7916..9079
                     /gene="pgk"
                     /locus_tag="KJL99_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HBD4435901.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            9294..10373
                     /gene="fbaA"
                     /locus_tag="KJL99_001098"
     CDS             9294..10373
                     /gene="fbaA"
                     /locus_tag="KJL99_001098"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HBD4435902.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            10564..11424
                     /gene="mscS"
                     /locus_tag="KJL99_001099"
     CDS             10564..11424
                     /gene="mscS"
                     /locus_tag="KJL99_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HBD4435903.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            11563..12198
                     /gene="argO"
                     /locus_tag="KJL99_001100"
     CDS             11563..12198
                     /gene="argO"
                     /locus_tag="KJL99_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HBD4435904.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            12291..13031
                     /locus_tag="KJL99_001101"
     CDS             12291..13031
                     /locus_tag="KJL99_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HBD4435905.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            13198..14148
                     /locus_tag="KJL99_001102"
     CDS             13198..14148
                     /locus_tag="KJL99_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBD4435906.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATIFSILLKIVTGQTINPASSRLQLNYGVSRRRG"
     gene            complement(14090..15568)
                     /gene="scpC"
                     /locus_tag="KJL99_001103"
                     /pseudo
     CDS             complement(14090..15568)
                     /gene="scpC"
                     /locus_tag="KJL99_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
     gene            complement(15592..16377)
                     /gene="scpB"
                     /locus_tag="KJL99_001104"
     CDS             complement(15592..16377)
                     /gene="scpB"
                     /locus_tag="KJL99_001104"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HBD4435907.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(16388..17383)
                     /gene="meaB"
                     /locus_tag="KJL99_001105"
     CDS             complement(16388..17383)
                     /gene="meaB"
                     /locus_tag="KJL99_001105"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HBD4435908.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGIAQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRGLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(17376..19520)
                     /gene="scpA"
                     /locus_tag="KJL99_001106"
     CDS             complement(17376..19520)
                     /gene="scpA"
                     /locus_tag="KJL99_001106"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HBD4435909.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSLF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCHTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(19724..20617)
                     /gene="argP"
                     /locus_tag="KJL99_001107"
     CDS             complement(19724..20617)
                     /gene="argP"
                     /locus_tag="KJL99_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HBD4435910.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            21000..21659
                     /gene="rpiA"
                     /locus_tag="KJL99_001108"
     CDS             21000..21659
                     /gene="rpiA"
                     /locus_tag="KJL99_001108"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HBD4435911.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            21915..23147
                     /gene="serA"
                     /locus_tag="KJL99_001109"
     CDS             21915..23147
                     /gene="serA"
                     /locus_tag="KJL99_001109"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HBD4435912.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            23376..23435
                     /gene="ibsC"
                     /locus_tag="KJL99_001110"
     CDS             23376..23435
                     /gene="ibsC"
                     /locus_tag="KJL99_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HBD4435913.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            23768..23827
                     /locus_tag="KJL99_001111"
     CDS             23768..23827
                     /locus_tag="KJL99_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HBD4435914.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(23928..24530)
                     /locus_tag="KJL99_001112"
     CDS             complement(23928..24530)
                     /locus_tag="KJL99_001112"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HBD4435915.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQLEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(24551..24734)
                     /gene="ssrS"
                     /locus_tag="KJL99_001113"
     ncRNA           complement(24551..24734)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="KJL99_001113"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24776..25105)
                     /gene="zapA"
                     /locus_tag="KJL99_001114"
     CDS             complement(24776..25105)
                     /gene="zapA"
                     /locus_tag="KJL99_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HBD4435916.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            25273..25851
                     /gene="ygfB"
                     /locus_tag="KJL99_001115"
     CDS             25273..25851
                     /gene="ygfB"
                     /locus_tag="KJL99_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HBD4435917.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            25877..27202
                     /gene="pepP"
                     /locus_tag="KJL99_001116"
     CDS             25877..27202
                     /gene="pepP"
                     /locus_tag="KJL99_001116"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HBD4435918.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     NVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            27199..28377
                     /gene="ubiH"
                     /locus_tag="KJL99_001117"
                     /gene_synonym="visB"
     CDS             27199..28377
                     /gene="ubiH"
                     /locus_tag="KJL99_001117"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HBD4435919.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            28400..29602
                     /gene="ubiI"
                     /locus_tag="KJL99_001118"
                     /gene_synonym="visC"
     CDS             28400..29602
                     /gene="ubiI"
                     /locus_tag="KJL99_001118"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HBD4435920.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAVDA
                     LPQLRVSAINAASEKLLTRLGVWQDILARRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPTELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(29676..29759)
                     /locus_tag="KJL99_001119"
     CDS             complement(29676..29759)
                     /locus_tag="KJL99_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4435921.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            30050..31144
                     /gene="gcvT"
                     /locus_tag="KJL99_001120"
     CDS             30050..31144
                     /gene="gcvT"
                     /locus_tag="KJL99_001120"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HBD4435922.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQVGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPTDRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            31168..31557
                     /gene="gcvH"
                     /locus_tag="KJL99_001121"
     CDS             31168..31557
                     /gene="gcvH"
                     /locus_tag="KJL99_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HBD4435923.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            31676..34549
                     /gene="gcvP"
                     /locus_tag="KJL99_001122"
     CDS             31676..34549
                     /gene="gcvP"
                     /locus_tag="KJL99_001122"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HBD4435924.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASNVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWTEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            34816..35559
                     /locus_tag="KJL99_001123"
     CDS             34816..35559
                     /locus_tag="KJL99_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBD4435925.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LIMQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDSLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(35616..37049)
                     /gene="bglA"
                     /locus_tag="KJL99_001124"
     CDS             complement(35616..37049)
                     /gene="bglA"
                     /locus_tag="KJL99_001124"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HBD4435926.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTCGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKFVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLCGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            37094..37404
                     /locus_tag="KJL99_001125"
                     /pseudo
     CDS             37094..37404
                     /locus_tag="KJL99_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
     gene            37568..38227
                     /locus_tag="KJL99_001126"
     CDS             37568..38227
                     /locus_tag="KJL99_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBD4435927.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(38423..39403)
                     /gene="ygfZ"
                     /locus_tag="KJL99_001127"
     CDS             complement(38423..39403)
                     /gene="ygfZ"
                     /locus_tag="KJL99_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HBD4435928.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            39646..39912
                     /gene="sdhE"
                     /locus_tag="KJL99_001128"
     CDS             39646..39912
                     /gene="sdhE"
                     /locus_tag="KJL99_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HBD4435929.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRNRERGPVAI"
     gene            39893..40300
                     /locus_tag="KJL99_001129"
     CDS             39893..40300
                     /locus_tag="KJL99_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HBD4435930.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(40340..40861)
                     /gene="fldB"
                     /locus_tag="KJL99_001130"
     CDS             complement(40340..40861)
                     /gene="fldB"
                     /locus_tag="KJL99_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HBD4435931.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIIALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            40973..41869
                     /gene="xerD"
                     /locus_tag="KJL99_001131"
     CDS             40973..41869
                     /gene="xerD"
                     /locus_tag="KJL99_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HBD4435932.1"
                     /translation="MKQELARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            41894..42604
                     /gene="dsbC"
                     /locus_tag="KJL99_001132"
     CDS             41894..42604
                     /gene="dsbC"
                     /locus_tag="KJL99_001132"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HBD4435933.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            42610..44343
                     /gene="recJ"
                     /locus_tag="KJL99_001133"
     CDS             42610..44343
                     /gene="recJ"
                     /locus_tag="KJL99_001133"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBD4435934.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            44434..45532
                     /gene="prfB"
                     /locus_tag="KJL99_001134"
     CDS             join(44434..44508,44510..45532)
                     /gene="prfB"
                     /locus_tag="KJL99_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HBD4435935.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            45542..47059
                     /gene="lysS"
                     /locus_tag="KJL99_001135"
     CDS             45542..47059
                     /gene="lysS"
                     /locus_tag="KJL99_001135"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBD4435936.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(47102..47650)
                     /gene="idi"
                     /locus_tag="KJL99_001136"
     CDS             complement(47102..47650)
                     /gene="idi"
                     /locus_tag="KJL99_001136"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HBD4435937.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPRGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            47705..47776
                     /locus_tag="KJL99_001137"
     CDS             47705..47776
                     /locus_tag="KJL99_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4435938.1"
                     /translation="MINQESVYRQPPVLSGCRQVKTI"
     gene            complement(47773..47898)
                     /locus_tag="KJL99_001138"
     CDS             complement(47773..47898)
                     /locus_tag="KJL99_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4435939.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(47900..49348)
                     /gene="uacT"
                     /locus_tag="KJL99_001139"
     CDS             complement(47900..49348)
                     /gene="uacT"
                     /locus_tag="KJL99_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HBD4435940.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIICGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVMRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLEVRTVRMWLLMRKLKKNEHGE"
BASE COUNT        12051 a        12452 c        13406 g        11921 t
ORIGIN      
        1 tttgaactgc cagaaattac cgataatgaa ctgttagtga gtgtaatttc tgacagcgtc
       61 tgtttatcga cctggaaagc ggcgttactc ggtagtgaac ataaacgcgt acccgacgat
      121 ttagaaaatc atccggtcat taccgggcat gaatgtgccg gggttattgt cgaagtgggt
      181 aaaaatctca ctggcaaata taaaaaaggc cagcgttttg tattgcaacc ggcgatgggg
      241 ttaccaagcg gatattcagc gggctacagc tacgaatatt ttggcggcaa tgccacttat
      301 atgattattc ccgaaatcgc cattaatttg ggctgcgtat taccgtatca cggctcttat
      361 tttgctgcgg cgtcgctggc agagcctatg tgctgcatta ttggtgctta tcatgccaat
      421 tatcacacca cgcaatatgt ttatgagcat cgcatgggcg tcaaacctgg cggcaatatt
      481 gcactgctgg cgtgtgcggg tccgatgggc attggcgcta tcgattacgc cattaacggt
      541 ggcatacaac cgtcacgggt ggtggtggtc gatatcgacg acaaacgcct ggcgcaggtg
      601 cagaagctgc tgccggtgga tctggcggcc agtaaaggca ttgagctggt gtatgtgaat
      661 accaaaggga tgagcgatcc tatccagatg ctgcgggcgc tgacaggaga tgtcgggttc
      721 gatgacattt ttgtttatgc ggcggtgcct gctgtcgttg agatggctga cgaattactg
      781 gcggaagatg gctgtctgaa cttctttgcc gggctgacgg ataaaaactt caaagtgccg
      841 tttaatttct acaacgtcca ttacaacagc acgcacgtag tcggtacatc cggcggttca
      901 acggacgaca tgaaagaggc gattgccctt agcgccacag gacagttaca gccgtcgttt
      961 atggtgaccc atattggtgg cctggatgcg gtgccagaaa ccgtgctcaa tctgccggat
     1021 atccctggcg gtaaaaaact catttataac ggcgtgacca tgccgctcac tgccattgcc
     1081 gattttgccg aaaaaggcaa aaccgatccg ctgtttaaag agttggcgcg gctggttgag
     1141 gaaacgcacg gcatctggaa tgaacaggcc gagaaatatc tgctggcaca atttggcgtt
     1201 gatatcgggg aggccgcgca atgatgtccc tggcgtggcc attatttcgc gttacggaac
     1261 aggcagcgtt ggctgcctgg ccgcaaaccg gatgtggcga caaaaataaa attgatggcc
     1321 tggcggtcac cgcgatgcgc caggcattaa acgacgtcgc ttttcgtgga cgagtggtta
     1381 tcggcgaagg agaaattgac catgcgccga tgctgtggat tggcgaagag gtgggcaaag
     1441 gcgatgggcc agaagtcgat atcgcggttg atcccattga aggcacgcgg atggtggcga
     1501 tggggcagag caatgcgctg gcggtcatgg ctttcgctcc tcgagatagc ctgttacatg
     1561 cgcccgatat gtatatgaaa aagctggttg ttaatcgact ggctgccggg gcgattgatc
     1621 tgtctttgcc tctggcggac aacttgcgta acgtggcgcg ggcgttaggt aaaccgctgg
     1681 acaagctgcg catggttacg ctggataaac cgcgtctgag tgccgcgatt gaagaagcaa
     1741 cacagctggg cgtgaaggtt tttgccctgc cggatggcga tgtcgccgcc agcgtgctga
     1801 cctgctggca ggataatcct tacgatgtga tgtacaccat cggcggtgcg ccagaaggcg
     1861 tgatttctgc ctgtgccgtt aaagcgttag gcggggatat gcaggccgag ctgattgact
     1921 tttgtcaggc taaaggggat tacacggaaa atcggcagat tgcagagcag gagcgcaagc
     1981 gttgtaaggc aatgggcgtt gacgttaacc gtgtgtactc gctcgatgaa ctggtgaggg
     2041 ggaacgatat cctctttagc gctacgggcg tgacgggcgg cgagttggtg aacggtatcc
     2101 aacagacggc gaatggggtg cggacgcaga cattactgat cggcggcgcg gaccaaacgt
     2161 gtaatataat agactccctg cattgatggt gatctatcgg aagaccaatg gcgacgctga
     2221 cagaagatga tgtgcttgag caactggatg cacaggacaa tttattttca tttatgaaaa
     2281 ctgcgcattc tattttgctc caggggatac gccagtttct gccgtcgctg tttgtcgata
     2341 acgatgaaga gatcgtcgaa tatgcagtga agccgttact cgcccaaagc ggcccgcttg
     2401 acgatattga tgttgcgctg cgtttgattt atgcgctggg gaaaatggat aaatggctgt
     2461 atgccgatat cacgcacttt tcccagtact ggcattacct gaacgaacag gatgaaacgc
     2521 ccggatttgc cgatgatata acctgggatt ttatcagcaa tgtcaacagt ataactcgca
     2581 atgcgacgct ctacgatgcg ttaaaagcga tgaagttcgc cgatttcgcc gtctggtcag
     2641 aggcgcgttt tagcggaatg gtcaaaacgg cgctgacgct ggcagtaacg accaccttaa
     2701 aggaattaac gccgtgaaaa ttgaattaac ggtgaatggg ctgaacgtgc aagcgcagta
     2761 ccatgatgaa gagatagaac gcgtgcataa actgttgctg cgtatgctgg cggccttaca
     2821 gacggtaaat ccgcagcggc gtacggtggt ttttctttgc gctccgccgg ggacgggtaa
     2881 atctacccta accaccttct gggaatatct cgcgcaacaa gacccggagc tgcccgctat
     2941 tcaggcgctc ccgatggatg gttttcacca ttacaatagc tggctggacg cgcatcaatt
     3001 gcgccccttc aaaggcgcac cagagacatt tgacgttgcg aaactggcgg aaaatctgcg
     3061 ccaggtggtg gaaggggatt gtacgtggcc gcagtacgat cgacaaaagc atgatcctgt
     3121 tgaagatgcg ttgcacgtta ccgcaccact cgtcatcgtc gaaggaaact ggttgttgct
     3181 ggacgacgag aagtggtgcc agcttgcgca attttgtgat ttctcgatct ttatcaacgc
     3241 gccagctgct gcgttgcggg agcgtctggt ggggcgcaag ctggcgggag gggtatcgct
     3301 ggcagatgca gaagcctttt atgaccgtac cgacgggccg aatgttcgcc gggggctgga
     3361 agagagtctg cctgcaaatt tgaccttaat gatgaccgct acgggtgaat atcgtctggt
     3421 ggattaacgt tcctgattac accatcttcc agccgcacca cacgagagaa atgacgccgg
     3481 gtaaatgcgg cgtcatgact gattgctacc acgctcgtgc ctcgctggcg gcacttctcc
     3541 agccagcttt caaacacgct caaccaattt tcgtcaaaat cccgactcgg ctcgtccagc
     3601 agtaatagtg gcggagagag tgcttccagg caggcgacgg cgaccatccg tcgctgcgca
     3661 ttatgcagat ccaacgggtg cgagttggca gtgtctgcta actgacaaca ttgcagggca
     3721 gcatgggtac gttgagtgat ttcatccgcc gggcattttt gcagtttcaa accaaatgcg
     3781 atctcgtcag cgacggtgct gtgaaaaagt tggttttccg cttcctgaaa cagtacgcca
     3841 acctttgccg cgcgctggcg gtttttcagg tttttcatcg cctgttgttg cagcataacc
     3901 gtaccggctg tgggcgtaag aagacctgcc attactcgta acagcgtcga tttccctgcg
     3961 ccattgtcac cggtcagcgc caaccattca ccttgtttga gttgcagaga aatgtcgcaa
     4021 agacaatccg ttgccgcgcc aggccagcga taagatatct tatttaacgt taacatagtg
     4081 gctggagcgc tccgttttgc aattgccacg cttgctggca gtagttcagg aaaggtgtgc
     4141 gatgacgttc aaaaagaata atcagcgaac cacgttcaaa cgcccagtgt tgtaagcgct
     4201 gcaacagcat ttcgctggcc tgcggtgtca ggcggctaaa ggcttcatca agaattaaga
     4261 gctttggctg catcgcgatg gcgcaggcga tgatcacgcg ctgtgtctct ccaccggaga
     4321 gtgttgccgg gtgacgatgg cgcaacggct ggcactcggt cagagtcagg gcggcatcaa
     4381 tccgcgccat aatctctgct tcagcaagac acagattttc tggcccaaag gcaacttctt
     4441 cttccacgct aaaggtacag ccggagagct gcaaataggg cgattgctgg acgagctgga
     4501 tggtggcgga ttgttcatta agcgggagat gcccaatggg cgtaccgagg agcgtccccg
     4561 tgcctgttat ctctcttggc agaaagtccg gataccagcc cgccatcagc tgtgccagcg
     4621 tacttttgcc actgccgttg tcaccaaaga tggcgaccat ccccggtttg acatagtgaa
     4681 agtcataaca gaacggagga tgcgttgagt gtgtcgggca ataacggaac tgttctaacg
     4741 taaccatatc cacgctccga attctgtcag catcagcaag atcatggtgt aacgcgctac
     4801 gcgttgtagg gtgctatcgg ctggtgccca caacgttgtt cgtcggttgt ggatccgaaa
     4861 tgcccgcata tccagcgccg caccacggac agccagatcg ttaagcgcat tgtgtgttag
     4921 cggaataatt aacgcgggca tagcacgtaa acgctgatac cagccctcat ccagcggcac
     4981 gccgcgggca cgctgcgctt catgaataat cgctaattgc cgttttaact gctcaacgac
     5041 caataacggc ccggcaaaca gataagcgac gcctggcggc aggcgagaag caaacagagc
     5101 gcgaataaaa cattgcacgg ggacgtactg cattcacaac tgtgaggttg aaacaatcgc
     5161 cagaatcagc aaccagagcg taatagcatg cgaccaccgt tctggcgagc gtggcgtgcc
     5221 gcttagccat tctggtagcc agccaccgtg taccagccac agcccggctc ccagcgagaa
     5281 catcagccag acgacatatt ttgcccgccg ccgcgtggcc tttagcgcaa taaggcagaa
     5341 aaaggtcgcc gcgctataga tcggcaaaat ggtctgcgcg ggcaggataa gcgtggtgca
     5401 ggccgctaat gcccacagtg ttaatgaggt aaatggatgc atttagcgca cggcagccat
     5461 tgccggaaaa tggcgcgtgg tgcgaatcgg cagctggcgc agtaaaagcc agacaatcac
     5521 cgctgtaaga attttatcga ccaggttcgc gcctatcacc gtaatcgcca ccgactccac
     5581 caggttttgc cccatcgagt gcatccaggc aacgaacaaa tcggctccgc taccggtgac
     5641 accgccgaac agcgcagtac gcagcggtac agcgacgacc gttaccgcaa gcgtaataat
     5701 cacgccgctg acaacgactt ttggcagggt gcgaaaccaa cccgctcgcg ccagccaacc
     5761 ggagaccagg ccgatgacca tcgcgacggg tgcaaatgcg gcggcgatcg ggtcggtcag
     5821 caatccccag agtaaattgg tcagtaagcc ggtcagcatt ccgatcaccg gaccaagcag
     5881 taccgcgcta ataagtgtgc cgatggagtc gagaaatata ggtaatttga ccatgctggc
     5941 cagttgcccg ccgatcatat tgatggcgat agatataacg atcaacacca gtgcctgact
     6001 ggaaaagtgg ctacgcgcca taagaaaacc tctttgaaag tagttattta acgggggaaa
     6061 tatgcaactc gtcgtaacca gaaagcgcgc aaggttggcg gctaaacgcc agttcctcaa
     6121 ggctaccgat caccagttca agcgtggtcc gcacggtaca gccgggcatc atatgaccgc
     6181 caagcatcgt gccgtgtgga tcggaaacgc agagatgaag atgctcacca ctctgttcca
     6241 gcgtgccgtt tagtgagatc acttcgaatg taccgttcag gagcgtggtg ccttcttgcc
     6301 cggcatagcg caaggcgacg tcagtcaggc taccggtaca gcctgcaatc taggcggcat
     6361 gaagttgatg ttgctgtgca aaagcgtgta gctgggaaaa aacttcctgc ccggggagga
     6421 ggcgcagtgc gtaaaagcgt gcggaggaag aatatgagta gggcaatgtc atcattggag
     6481 tactccgaaa ctcggacgta ttgcggataa tatcgacgct gcatcagtca tattctgata
     6541 taagtcaggg tttgtgacgg cacaacgaaa agattttgct acgcttattc gtgattctgc
     6601 aaagtggaat aaattccaca ggggcggggc tgaaaacgtg acgtaagtca gataattgct
     6661 ttctttgact ggacaatcat tccttttatt ccacgtttcg cttatcctag ctgaagcgtt
     6721 tcagtcgatt aaatgttcga caattaacca atcagtcgca gtttgcgaca ggtaaggttt
     6781 ccccggacga tttgctggat tactctgtct ggcaaatttg ctcagggaaa accttgcagg
     6841 agatctatga ccgtacgcgt agcgataaat ggcttcggtc gcatcgggcg taatgtggtt
     6901 cgtgctttgt atgaatccgg acgccgggcg gaaattaccg tggtggcaat caacgaactg
     6961 gcggatgctg cgggcatggc gcatttgttg aaatatgaca ccagccatgg ccgttttgca
     7021 tgggaagtac gacaggaacg cgatcaactt tttgttggtg atgacgccat ccgcgtattg
     7081 catgaacgtt cactgcaatc gctcccctgg cgtgaacttg gcgttgatgt agtcctcgac
     7141 tgcaccggcg tatatggctc ccgcgagcat ggcgaagcgc atattgccgc cggggccaaa
     7201 aaagtgctct tttcacatcc tggcagtaac gatctcgacg cgaccgttgt ttacggcgtc
     7261 aatcaggatc aacttcgtgc ggaacaccgc atcgtttcta acgcttcctg taccacgaat
     7321 tgcataattc ccgtcatcaa attgttagat gatgcgtacg gtattgagtc cggcactgtg
     7381 accacaattc actccgccat gcacgatcaa caggttattg atgcatacca tcctgacctg
     7441 cgtcgcaccc gggcagccag ccagtcgatc attccggtcg atactaaact ggccgctggt
     7501 atcacacgat tttttccgca atttaacgat cgctttgaag cgattgcggt acgtgtgcca
     7561 accataaatg tgacggcaat cgatttaagc gtgacggtga agaaacctgt aaaagccaat
     7621 gaagtcaacc tgttgctgca aaaagcagca caaggtgcat ttcatggtat agttgactat
     7681 acggaattgc cgttggtctc tgtagatttt aaccacgatc cgcacagtgc cattgtcgat
     7741 ggcacccaaa cccgggtcag tggcgcacac ctgatcaaaa cgttggtctg gtgcgataac
     7801 gaatggggct ttgctaaccg aatgctcgac acgacgttag ctatggctac tgttgctttc
     7861 aggtaagacg caagcagcgt ctgcaaaact tttagaatca acgagaggat tcaccatgtc
     7921 tgtaattaag atgaccgatc tggatcttgc tgggaaacgt gtatttatcc gtgcggatct
     7981 gaacgtacca gtaaaagacg ggaaagtaac cagcgacgcg cgtatccgtg cttctctgcc
     8041 gaccatcgaa ctggccctga aacaaggcgc aaaagtgatg gtaacttccc acctgggtcg
     8101 tcctaccgaa ggcgagtaca acgaagaatt ctctctgctg ccggttgtta actacctgaa
     8161 agacaaactg tctaacccgg ttcgtctggt taaagattac ctcgacggcg ttgacgttgc
     8221 tgaaggtgaa ctggttgttc tggaaaacgt tcgcttcaac aaaggcgaga agaaagacga
     8281 cgaaaccctg tccaaaaaat acgctgcact gtgtgacgtg ttcgtaatgg acgcattcgg
     8341 tactgctcac cgcgcgcagg cttctactca cggtatcggt aaattcgctg acgttgcgtg
     8401 cgcaggcccg ctgctggcag ctgaactgga cgcgctgggt aaagcactga aagaacctgc
     8461 tcgcccgatg gtggctatcg ttggtggttc taaagtatct accaaactga ccgttctgga
     8521 ctccctgtct aaaatcgctg accagctgat tgttggtggt ggtatcgcta acacctttat
     8581 cgcggcacaa ggccacgatg tgggtaaatc cctgtacgaa gctgacctgg ttgacgaagc
     8641 taaacgtctg ctgaccacct gcaacatccc ggttccgtct gatgttcgcg tagcaaccga
     8701 gttctctgaa actgcaccgg ctaccctgaa atctgttaac gatgtgaaag ctgacgagca
     8761 gatcctggat atcggtgatg cttccgctca ggaactggct gaaatcctga agaatgcgaa
     8821 aaccattctg tggaacggtc cggttggcgt gttcgaattc ccgaacttcc gcaaaggtac
     8881 tgaaatcgtg gctaacgcta tcgcagacag cgaagcgttc tccatcgctg gcggcggcga
     8941 cactctggca gcaatcgacc tgttcggcat tgctgacaaa atctcctaca tctccactgg
     9001 cggcggcgca ttcctcgaat tcgtggaagg taaagtactg cctgcagtag cgatgctcga
     9061 agagcgcgct aagaagtaaa aaatcacagg gcagggaaac ctgcacttgt ttcagcgcgc
     9121 ttttagagca cgcatttttt catactctaa ataattcgag ttgcaggaag gcgacaagag
     9181 agtgaatccc caggagctta cataagtaag tgactggggt gagcgaacgc agacgcagca
     9241 catgcaactt gaagtattat gagtataagg cccgacgata caggacaaga gacatgtcta
     9301 agatttttga tttcgtaaaa cctggcgtaa tcactggtga tgacgtacag aaagttttcc
     9361 aggtagcaaa agaaaacaac ttcgcactgc cagcagtaaa ctgcgtcggt actgactcca
     9421 tcaacgccgt actggaaact gctgctaaag ttaaagcgcc ggttatcgtt cagttctcca
     9481 acggtggtgc ttcctttatc gctggtaaag gcgtgaaatc tgacgttccg cagggtgctg
     9541 ctatcctggg cgcgatctct ggtgcgcatc acgttcacca gatggctgaa cattatggtg
     9601 ttccggttat cctgcacact gaccactgcg cgaagaaact gctgccgtgg atcgacggtc
     9661 tgctggacgc gggtgaaaaa cacttcgcag ctaccggtaa gccgctgttc tcttctcaca
     9721 tgatcgacct gtctgaagaa tctctgcaag agaacatcga aatctgctct aaatacctgg
     9781 agcgtatgtc caaaatcggc atgactctgg aaatcgaact gggttgcacc ggtggtgaag
     9841 aagacggcgt ggacaacagc cacatggacg cttctgcact gtacacccag ccggaagacg
     9901 ttgattacgc atacaccgag ctgagcaaaa tcagcccgcg tttcaccatc gctgcgtcct
     9961 tcggtaacgt acacggtgtt tacaagccgg gtaacgtggt tctgactccg accatcctgc
    10021 gtgattctca ggaatatgtt tctaagaaac acaacctgcc gcacaacagc ctgaacttcg
    10081 tattccacgg tggttccggt tctactgctc aggaaatcaa agactccgta agctacggcg
    10141 tagtgaaaat gaacatcgat accgataccc aatgggcaac ctgggaaggc gttctgaact
    10201 actacaaagc gaacgaagct tatctgcagg gtcagctggg taacccgaaa ggcgaagatc
    10261 agccgaacaa gaaatactac gatccgcgcg tatggctgcg tgccggtcag acttcgatga
    10321 tcgctcgtct ggaaaaagca ttccaggaac tgaacgcgat cgacgttctg taagtcattc
    10381 ctttctgctt atctcaaggc ccgctctgcg ggtctttttt tcgccaaaag caaggtgatt
    10441 cagatgagaa atctgtgatc tatttggcaa aattatgctt tattgtttac ccttgtcaga
    10501 ctgcccgtca taaggcggcg gagtgtattt ctccattttg agtcagttga aaaggaatat
    10561 tgaatggaag atttgaatgt tgtcgatagc ataaacggcg cgggaagctg gctggtagct
    10621 aaccaggcgc tgctgctaag ttatgcagta aacatcgtgg cggcactcgc gatcatcatc
    10681 gttggtttga ttatcgcgcg gatgatttcc aacgcggtga atcgcctgat gatctcccgt
    10741 aaaatcgatg ccactgttgc tgattttctt tctgcattag tccgttacgg tattatcgcc
    10801 tttacgctaa tcgctgccct gggacgcgtg ggtgtacaaa cagcgtcagt cattgctgta
    10861 ctcggtgccg caggcttagc tgttggtctg gctttgcagg ggtcactttc taacctggcc
    10921 gctggcgtgt tacttgtcat gttccgcccg ttccgtgccg gagaatatgt tgacctcggc
    10981 ggcgtagccg gtactgtgct gagtgtgcag attttctcca ccaccatgcg tactgcagac
    11041 ggtaaaatta tcgttattcc gaacggtaaa attattgccg gaaatattat taacttctcc
    11101 cgcgagccag ttcgccgtaa cgaatttatt attggcgtgg cgtatgattc cgatatcgat
    11161 caggttaagc agatcctgac caatattatc cagtctgaag atcgcatttt gaaagatcgc
    11221 gaaatgactg tgcgcctgaa cgaacttggt gcatcgtcga ttaatttcgt ggtccgcgtc
    11281 tggagcaaca gcggcgatct gcaaaacgtg tactgggatg tgctggagcg tattaaacgt
    11341 gaatttgatg ccgccggtat cagcttcccg tacccgcaaa tggatgtgaa ctttaagcgg
    11401 gtgaaagaag acaaagctgc gtaatcaacg ctgaggccag ataatactct ctggcctctc
    11461 tcttattagt tattctgatt gccaattaat attatcaatt tccgctaata acaatcccgc
    11521 gatatagtct ctgcatcaga tacttaattc ggaatatcca acgtgttttc ttattacttt
    11581 caaggtcttg ctcttggggc agctatgatt ctgccgctcg gcccacaaaa tgcttttgtg
    11641 atgaatcagg gcattcgtcg tcagtaccac attatgattg ccttactttg cgctatcagc
    11701 gatttggtcc tgatttgcgc ggggattttt ggtggtagcg cgttattgat gcagtcgccg
    11761 tggttgctag cgctggtcac ctggggcggc gtagtcttct tgctctggta tggttttggc
    11821 gcttttaaaa cagcgatgag tagtaatatt gagttagcta gtgccgaagt cctgaagcaa
    11881 ggcagatgga aaattatcgc caccatgttg gcagtgacct ggctgaatcc gcatgtttac
    11941 ctggatactt ttgttgtact gggcagcctt ggcgggcagc ttgacgtcga accaaaacgt
    12001 tggtttgcgc tcgggacaat tagcgcctct ttcctgtggt tctttggtct ggctattctc
    12061 gcagcctggc tggcaccgcg cctgcgcacg gcaaaatcac agcgcattat caatctggta
    12121 gtgggatgtg tcatgtggtt tatagccttg cagctggcga gagacggtat tgctcatgca
    12181 caagccttgt tcagttaggc gctatctgat ggaaaaataa aacagaggcg ctaagcttgc
    12241 ctccagaggt cctgaatttt gggcaagtca attaacacgg agagactaac gtgaagttca
    12301 aagttatcgc cctggcggca ttaatgggta ttagcgggat ggcagcgcag gctaacgaat
    12361 tgccggatgg accgcatatt gtcacctccg gtacggcaag tgtggatgcg gtgccagaca
    12421 ttgccactct tgcgattgaa gttaacgtgg ccgcgaagga tgccgctaca gccaagaaac
    12481 aggcagatga gcgcgtcgca caatacattt ccttccttga actcaatcag atcgcgaaaa
    12541 aagatatcag ctcagcgaat ttacgcaccc agccagatta tgactatcag gatggtaaaa
    12601 gtatcctgaa aggctaccga gctgtgagaa cggtggaagt cacgctccgt cagttagaca
    12661 aactgaattc cttgctggat ggcgcgctga aggcgggtct taacgaaatt cgttctgtgt
    12721 cgctgggcgt ggcgcagccg gatgcctata aagacaaagc gcgtaaggca gcgattgata
    12781 acgcgattca tcaggcgcag gaactggcga acggctttca tcgtaaactg gggccggtat
    12841 atagcgtgcg ctaccatgtt tccaactatc agcccagccc aatggtgcgg atgatgaaag
    12901 ccgatgccgc gccggtgtct gcccaggaaa cttacgagca ggccgctatt cagtttgatg
    12961 atcaggtcga tgtggtcttc cagttagaac ctgtggatca acaacccgct aaaacacctg
    13021 cagcacaata atacttgtag cccgacgtat tcgtcgggct aatgccttta cccgatattg
    13081 cctccttttt ctgtaatttt ctgtaaaacc atgacgtaat cacctatctt tgctcgcggt
    13141 ttcatcaagg ataggctgac tgaaacggtt aagcggacaa ggatgcgggg aggggggatg
    13201 gacattttta tttcgaaaaa gatgcgcaat ttcattttat tagcgcaaac caataatatt
    13261 gcccgggcgg cagaaaaaat ccatatgacg gcttcgccat ttggtaaaag cattgccgcg
    13321 ctggaagaac aaattggcta tacgctattt acccgcaaag ataataatat cagcctcaat
    13381 aaagccggtc aggaactgta tcaaaaactg ttcccggttt atcaacgact ttctgcgatt
    13441 gataatgaaa tccataactc agggcgtcgt tcacgagaaa ttgtgattgg catagataat
    13501 acttatccga caattatttt cgatcagttg attagtctcg gagataagta tgaaggcgtg
    13561 accgcccagc cagttgagtt cagcgaaaac ggcgttatcg ataatctttt tgaccgtcag
    13621 ttagatttta ttatctcccc acagcatgta tctgcacgag ttcaggaact ggaaaacctg
    13681 actatcagtg aattaccacc gctgcgattg ggctttctcg tttctcgccg ttatgaagag
    13741 cgtcaagagc aagagctgct tcaggagctg ccctggctac aaatgcgctt tcaaaaccgg
    13801 gctaacttcg aagcaatgat tgatgcgaat atgcgacctt gtgggatcaa tcccaccatt
    13861 atttatcgtc cctacagttt tatggccaaa atcagcgccg tggagcgcgg acattttctg
    13921 acggtgatcc cgcattttgc ctggcgactg gtgaatccgg caacgctgaa atattttgat
    13981 gcgccgcaca ggccgatgta tatgcaggaa tatctttatt caatcagaaa tcatcgttat
    14041 accgccacga tcttcagcat attgctgaag atcgtgacgg gacaaaccat taacccagca
    14101 tcgagccggt tgcaattaaa ttacggtgta agtcgaagac gtggctaaga tcgtggtgaa
    14161 tatgtccgcc gggcgcattt tccagatagc gatgcagata atcccgatac ataggatgtg
    14221 cacaattatc aatgatagtg cgggcgcgtt gaagcgggga aagaccgcgc agatcggcga
    14281 tcccttgttc ggtgatgatc actttgacgc tgtgttcgct gtgatcaaca tggctgcaca
    14341 ttggcacgac ggttgagatc ttgccttctt tagcaatcga cggagccata aagatcgaca
    14401 gatacgcgtt gcgttcaaaa tcaccgctgc cgccgatgcc gttcatcaga tcgaccccgg
    14461 ctacgtgcgt tgaatttgca tgcccgtaaa tatcaaactc caggccgacg ttcagagcga
    14521 tgacgcccag acgacggatg atttccgggt tattggaaat ctcctgcgga cgcaacacaa
    14581 tgcggctggc aaagtaatcc atattgtcgt aaatcttgcg cagggaatcg gccgagattg
    14641 tcaggctgga ggcgctggcc ccgctgattt tgccggtttc cagtaaatgc accaccgatt
    14701 cctgtagcac ttccgaatac atcatgaacg gaggaatttc cgggttttcc cccagacgtg
    14761 ccattaccgc attattgata ttgcccacgc cactttgcag cggcagaaat tccggcggaa
    14821 tacgcccatg cgccatttcc tgcaataaga acgtgaccac gttatcggca atctgctggc
    14881 acatgggatt ttgcttatcc agcatattac cggcgtcggg caagttggtt tccacgacgg
    14941 cgacaatctt tttcggatcg atttgcacat agcgggtacc gacgcgatcc attgcatgga
    15001 agatcgacac gctattgcgc cgcggtggcg cgccaggaat cacaatatct gccagttctg
    15061 caacgcgcgg atcgtgatag tgattaagtt caatgatcac tttcttcgct cgcagcagct
    15121 aggtcggcgc attaccgatc ccgctggtta accagactcg accatccggt gccagtgccg
    15181 atgcttcaat gacggcaaca tcaatgtcgc cgaagaaacc gtaattgacc atttgcgcca
    15241 cttcgctcaa atgcaggtca acgaaactca ccgcgccctg attgatcttt ttacgtaaac
    15301 tggacgatgt ttgatatggc gcacgccagg aaacagcatc ggcgtcagaa agtacatcgt
    15361 cagcggcggc gctgattgac gcccccgtca gcaggcgaat ttgatacggc tttttggcct
    15421 catgctgttc gttagctctg cgggcaatcg cggcgggtag ggctttcggc gaacccgctg
    15481 gggtaaagcc gctaaatgcc accatgtcgt tatgctggat aatttctgcc gcttcatcgg
    15541 cggtcatcct tgtccactga gtttccattt ttactccttc gttcgcaggg attaatgacc
    15601 aacgaaatta ggtttacgtt tttcgaggaa agcgttcatc ccttcctggt aatcttcgct
    15661 gtcataaacc gcgcggcgca tcccctgaat acgttcaaat tcatcggagt tcatggtgtg
    15721 tgcttcgccc agtacacgca gctcttcttt gataacggca atggctaacg gcgctttctc
    15781 agagatgtgg tgcgccattt gtaaggtgaa atcttccagt tcttccactt ccacaacatg
    15841 gttgaggatg ccgacagcca gcgcgcgctg ggcggtgatt ggcgaagcgg taaaaatcag
    15901 ctctttgaca atgtggaagc ccgcgtcgcg ggtcaggttg tgaatgccga ccaggttata
    15961 cgggacgccg aggtttacag gcgtcattga gaaggttgag gtactggcgg cgatgatcag
    16021 atcggaactc atgatcattt caaatgcgcc accccaaaca ctaccttcca ccatcgaaat
    16081 gatcggtttc gggaattttt ggatcatgcg ggtgatttga cgcaatggat catcatagga
    16141 gagcggatcg cgaccgccag acggcagttc gtgaatatcg tgacctgcgg agaagacttt
    16201 ggatccactt ggcgcccgca aaatgataca gcgaatttcc ggccggttga gatcgcttaa
    16261 cgcctgcata agatcatcaa taaagacttt acttaaggca ttaagttttc gtccatagtt
    16321 aaactcaatg accgccactt tgttgatagt ggcaacgttc acatactgat aagacataaa
    16381 aattccttta atcaaaatat tgcgtctgga taaattcact gagctgccgc aggccggtgc
    16441 gcggtgagag cgtattgttt ttgaccgcta aaagcgtctg gcggtaatag cgatcgaaat
    16501 cttcattcgc gaacaggtga ttcagtactt cttcttcggt ctgcttacgc agccattcca
    16561 ccgattgttg ttgccgcact tgttgtaaac gaccactggc agttagcgcg gttttgaagt
    16621 cgatgatggc gtgccagatc tcatcgattc cacgtttttc cagtgcgcta caagtcagac
    16681 cccgtggctg ccattcgtcg tatttacgtc gcagaatatg cagggcactc tcgtacatat
    16741 gccgggcaat ggcgacattg gtatggttat cgccatcgtc tttgttgata acgagcagat
    16801 cagccacttc catcagccct tttttaatgc cctgcagatc atcgccgcca ccggcaattt
    16861 gcaacgagat aaaacagtcc accatgcggg cgacttccgt ttccgactgc gcgatgccaa
    16921 ccgtttcgac aatcactacg tcataacccg ccgcttcgca taacagcatt aattcccgtg
    16981 ctcgctgact ggcaccgccc agatgaccgg aggagggtac cgggcgaata aacgccgcct
    17041 cggcacgcgc caggtcattc atgcgggttt tatccccgag aatgctaccg ccagtgaccg
    17101 ggctgctggg atcgaccgca ataacagcaa cctttaatcc ctcacgaatc aacaacatgc
    17161 caaaggcttc aagaaaggta cttttccccg cgccgggtgt gccggtaacg cccagtcgca
    17221 gggcgttacc acagaacggc ataatagcgt caagcagctg tgtacttagc gcctgatgac
    17281 gtgggtgacg gctttccacc agcgtcatgg cttgggcgag cgtggcacgc tcaccctgac
    17341 gtaagcggcg aatactttct gccagcgtgg cttcattaat catgatgctg gcttatcaga
    17401 ttcagtacgt cgcgcacact gtcgagcata ggtgtacctg gaccataaat cgccgccacg
    17461 ccgcgctctt gcaggaaggc gtaatcctgc ggcggaatga cgccacccgc gaccacgcag
    17521 atatcttcgc gtccccattt tttcagcgct tcgaccagtt ccgggatcag cgttttatga
    17581 ccggcagcca gtgaggatgc gcccactacg tgaacgtcgt tttctacggc taggcgggcg
    17641 atctcttcag gtgtagagaa catcgggctt aaatctacgt cgaaaccgag atcggaatag
    17701 gcgctggcga tcactttcgc gccgcgatcg tgtccatcct ggcccatctt agcgatcaga
    17761 atgcgcgggc gacgaccatt gtcggcaagg aactgctccg tttgcgcaac aatggcatcg
    17821 aactcggagg ccgatttctc agactgatga tagctttgcg caatcacgcc ggtaacacac
    17881 tggcttggca ccagataacg gtcgaaagcg acttccagcg catcggaaat ttcacccagg
    17941 gtggcgcgaa cgcgagcggc attaacagcg gcagccagca ggttttcgtt atgctgtgcg
    18001 gcgtgagtca gggcgttcaa cgcggcggtt acggcggcat catcacgggt ggcgcgaatg
    18061 cgttccagcg aagcaatttg ctcgttacgc accatcacgt tgtcgatctc aagtacatcg
    18121 gtttcgtctt cgtgatccag tttgtacttg ttgacaccaa cgatgacacg cttgccctgg
    18181 tcgatcagcg actgttcgcg cgctgaggcc tcttcgatca ttcgttttgg cagacctgct
    18241 tcgatcgctt tcgccatgcc accggcttcg tcgatctgtt ggataatagc tctggcttgt
    18301 ttgacgattt gatcggtcag cgactcaatg taataggatc cggccagtgg atcgacggtg
    18361 tggcagagtt ctgattcttc ctggatgatg atctgggtgt tgcgggcaat gcgtgctgag
    18421 aaatcggtag gcaaaccaag cgcttcgtca aaggcgttgg tatgcagtga ctgagtaccg
    18481 cccagcgtcg cagccagtgc ttcaatggtg gtgcggataa cgttgttata cggatcctgt
    18541 tcagtcaggc tccagcctga ggtctggcag tgggtacgca gcgccagtga tttcggatcc
    18601 tgtgcgccaa atccactgac cgcttcgctc cataaataac gtgccgcacg caacatggcg
    18661 acgttcataa acagatccat gccgataccg aagaataacg acaggcgagg agcgaagtca
    18721 tcaattttca gtccggcaga gattgctgct ttgatgtact caatcccatc ggcgagcgta
    18781 aatgctacct gctgcacgca gttggcaccc gcttccccca tgtggtaacc gctgatactg
    18841 atggtattaa atcgcggcat gttgccggaa caccaggcga tgatgtcggc gataatgcgc
    18901 attgacggtt ttggtgggta aatataggtg ttgcggcaga ggtactcttt gagaatatcg
    18961 ttttgaatgg tgccggtcag tttatcaggt gtaacacctt gctcttctgc ggcgacgata
    19021 taaaacgcca gtactggtag cactgcgcca ttcatggtca tcgaaaccga cattttatcc
    19081 agcgggatct ggtcgaacag gactttcata tcttccacgg tgtcgatagc gacgcccgct
    19141 ttgccgacgt cacccgccac gcgtgggtta tcggagtcgt agccacggtg ggtggcaagg
    19201 tcaaacgcaa cggaaagacc tttttgcccg gcggccaggt tacggcgata aaaagcgttg
    19261 gactcttttg ctgttgaaaa accagcatac tgacggatgg tccacggttg ggcggtatac
    19321 atagtggcac gcgggccacg aacgtagggc ggcaaaccag gaagggtacc tgtcacctcc
    19381 agattatcga gatcggcttc ggtatacagc ggcttgatgg cgatcccttc cgcggtttgc
    19441 tgaaccagcg agtcgacagt tttctcccga cggctcaatt ccttgttggc aagctgttgc
    19501 cactcctgct cgttagacat tatttgctcc gtaaaacgca atcaaaatga gggtatttgc
    19561 gcttacggtg agctttttac gtgggtttgt cgccagcaaa aaaaatgcat tcagacgtcg
    19621 ttttttgccg cctgagattt gtctattttg tgatgtgatt caaggaatac aaaaagaagt
    19681 gaggacaacg cctgatatgt gctatcaggc attatttgat ggattaatcc tgacgaagga
    19741 ctttgtgacc ataatcgagt aacgcatcag tgactttacg catcatgcgg ctttcaggag
    19801 caaagcggtg ccagtagagc atccgtcgtt gaaatagccc aggcgttaag tcaatcagtt
    19861 caccgctggc cagctctttc tcgatttgca ggtgcgggat catacagcag gtggtgccct
    19921 ggcgagcaag ttgtacgaac gcttctgaag aattaacgat atggcagggt acgctgccag
    19981 gaggaagatc gaagttttgc tgcaaaaagg cctggtgcat atcgtcaaga tggtcaaacg
    20041 cgaccactgg cgctttcaat aatgccgaac gcgttacgcc gttaggaaaa tatttttcgg
    20101 caaacggttt tgagctgacg aacagatagt cgagcgcacc gagtttatcg acaagacaac
    20161 tcggcagcgc ctgatgttga atactcaccg cgccgaccac ttcgccgcgg cgcagacgtt
    20221 cctgagtacg ggtttcatct tctacctgca agttgaggcg gataggcgaa tcagccaaca
    20281 caggagccag tgccggaagc aaccacgtcg ccagactgtc ggcgttgacc gccagtgaaa
    20341 gcagcagcgg agtcgaaccg gtttgttcat cgcccagcca ctcttcttcc agcaactcca
    20401 cctggcgcag cagtgccagc agtttttgcc cttgttccgt cgggcgcggc ggtacggtac
    20461 gcaccaacag cggctgcccg aacatatttt ccagttgctt aatgcgctgt gagacggctg
    20521 attgtgtaat gcacagcttt tgtgccgcgc gctcaaatcc tcgttcacgt atcaccgcat
    20581 ccagtgcctg taatgttctg tagtccgggc gtttcattgc tgttgtctgc tcctggtaaa
    20641 tgctttttcc tgcactatga cacaattttg tgtcaggttg catataccga agccgtcggc
    20701 gggtaacatt tgtatcaatg gttaaacgcg cagcttaaaa gatcagggat ttgcgctatt
    20761 gcatttcgct tagcgacttc gcgccctggt tatgttgaat ggcgtggcgt tattgcctct
    20821 gttcgcaccg tggagcggca tacccagacc gttgtatgct ttatttgcgg ggcgaaaggg
    20881 gatgcccgcc attgcgcgat attttatgga tgagttaacc acccggcttg ccaacggggt
    20941 ctgaatcgct ttttttgtat ataatgcgtg tgaaatttca taccacaggc gaaacgatca
    21001 tgacgcagga tgaattgaaa aaagcagtag gatgggcggc acttcagtat gttcagcccg
    21061 gtaccattgt tggtgtaggt acaggttcca ccgccgcaca ctttattgac gcgctcggca
    21121 cgatgaaagg ccagattgaa ggggccgttt ccagttcaga tgcttccact gaaaaactga
    21181 aaagcctcgg cattcacgtt tttgatctca acgaagtcga cagccttggc atctacgttg
    21241 atggcgcaga tgaaatcaac ggccacatgc aaatgatcaa aggcggcggc gcggcgctga
    21301 cccgtgaaaa aatcattgct tcggttgcgg aaaaatttat ctgtattgcc gacgcctcca
    21361 agcaggttga tattctgggt aaattcccgc tgccagtaga agttatcccg atggcacgta
    21421 gtgcagtggc gcgccagctg gtgaaactgg gcggtcgtcc ggaataccgt cagggcgtgg
    21481 tgaccgataa tggcaacgtg atcctcgacg tccacggcat ggaaatcctt gacccgatag
    21541 cgatggaaaa cgccataaat gcgattcctg gcgtggtgac tgttggcttg tttgctaacc
    21601 gtggcgcgga cgttgcgctg attggcacac ctgacggtgt caaaaccatt gtgaaatgat
    21661 ctgacggggg aacctccccc gttaaaaaaa ttctcttcat taaatttggt gacatgtgtc
    21721 acgcttttac caggcaattg tcgattgctc taaataaatc ctctaaacca gcatattcat
    21781 ccaagaatta cctttgcgtg atatttcctc aacatcgcga cgcaaacgtt catattgccg
    21841 caatattatt ttttgatatg ttgaaaggcg gatgcaaatc cgcacacaac atttcaaaag
    21901 acaggattgg gtaaatggca aaggtatcgc tggagaaaga caagattaag tttctgctgg
    21961 tagaaggcgt gcaccaaaag gcgctggaaa gccttcgtgc agctggttac accaacatcg
    22021 aatttcacaa aggcgcgctg gatgatgaac aattaaaaga atccatccgc gatgcccact
    22081 tcatcggcct gcgatcccgt acccatctga ctgaagacgt gatcaacgcc gcagaaaaac
    22141 tggtcgctat tggctgtttc tgtatcggaa caaaccaggt tgatctggat gcggcggcaa
    22201 agcgcgggat cccggtattt aacgcaccgt tctcaaatac gcgctctgtt gcggagctgg
    22261 tgattggcga actgctgctg ctattgcgcg gcgtgccgga agccaatgct aaagcgcacc
    22321 gtggcgtgtg gaacaaactg gcggcgggtt cttttgaagc gcgcggcaaa aagctgggta
    22381 tcatcggcta cggtcatatt ggtacgcaat tgggcattct ggctgaatcg ctgggaatgt
    22441 atgtttactt ttatgatatt gaaaacaaac tgccgctggg caacgccact caggtacagc
    22501 atctttctga cctgctgaat atgagcgatg tggtgagtct gcatgtacca gagaatccgt
    22561 ccaccaaaaa tatgatgggc gcgaaagaga tttcgctaat gaagcccggc tcgctgctga
    22621 ttaatgcctc gcgcggtact gtggtggata ttccggcgct gtgtgatgcg ctggcgagca
    22681 aacatctggc gggggcggca atcgacgtat tcccgacgga accggcgacc aatagcgatc
    22741 catttacctc tccactgtgt gaattcgaca acgtccttct gacgccacac attggcggtt
    22801 cgactcagga agcgcaggag aatatcggct tggaagttgc gggtaaattg atcaagtatt
    22861 ctgacaatgg ctcaacgctc tctgcggtga acttcccgga agtctcgctg ccactgcatg
    22921 gtggtcgtcg tctgatgcac atccacgaaa accgtccggg cgtgctaact gcgctgaaca
    22981 aaatcttcgc cgagcagggc gtcaacatcg ccgcgcaata tctacaaact tccgcccaga
    23041 tgggttatgt agttattgat attgaagctg acgaagacgt tgccgaaaaa gcgctgcagg
    23101 caatgaaagc tattccgggt accattcgcg cccgtctgct gtactaattc cccttctctg
    23161 aaaattaacg ggcaggtcac tgacttgccc gtttttttat cccttctcca caccgttgcc
    23221 gcgttgcgca ttctactttg cgagtctgct ccagaaaata aatcaaagtc agatcaatgc
    23281 gttgtgttta tttaatgggg atttacgatg gcgggacagc atggggctgt aacggtaaag
    23341 ccctcaccga agcgagggct taaaggagaa gggttatgat gcgacttgtc atcatactga
    23401 ttgtactgtt actgataagt ttcggagctt attaacagtc agtctcaagg gaggagtcat
    23461 cctcccttac ccttactcac taaattaggt caaagaatca acgatgtcaa tcagggcgat
    23521 gcggggtgca tcgccctgaa ccgaactttt tgatagccac ttcatcgccc acgtttactg
    23581 ccgtaataga tcctttttcc caaaaattat ctctgttttg catggggttt ttcgagcacg
    23641 ctcgaagaaa agatttaaag gtagatcaat gcgttgtggt tatttaaaga gagcttacga
    23701 tagctgggca gcatggggct gtaacggtaa agccctcacc gaagcgaggg cttgaaggag
    23761 aagggttatg atgcgacttg ccatcatact gattgtactg ttactcataa gtttcccagc
    23821 ttattaacag tcagtcacag gggaggagca atcctccctt acccttactc actaaattag
    23881 gttaaagaat caacgatgtc aatcagggcg atgcaggtgt atcgccctta ccactcccag
    23941 actttcgacg gtgtaaccac cgcaggaaga gggatatccc actcttcaac ggggagtttt
    24001 tccaccaact gacaatcatg cgcataaccc accggttgcg ttttatagtg ctgccagttt
    24061 tgtaaggtcc gatcataaaa accaccgccc attcccagac gctgaccgta ctcatcaaag
    24121 gcgaccagcg gtgtgatcag cacgtctaat cgggaaaggg gtagcacgtc acgcacatcc
    24181 agttttggct catggatctt caacctgttc atcaccagtt cgctttgcgg atggtaattc
    24241 aggaacagca aattaccggc actaaaggga tgcaaaactg gaagatatac gcgcttaccg
    24301 gcgcgccaga gttgttctat tagcggctgg gtgtcgagtt caccatcaaa agagaggaat
    24361 acagcgaccg tatgcgccat caccaccggg ggataagtca tcatccgggt agcggcttgt
    24421 tgacccattt ccagctgttg ttccggcgtt aacgcacgac gacgttgccg aatcattttg
    24481 cggatttctt gtcgggataa tgtcagtggg agctctggta gttgcgtcat ggctggtgcc
    24541 agataagaag ggaatctccg agatgccgcc gcaggctgta acccttgaac ccttggttca
    24601 aggtgaatgt gtcgtcgcag ttttaaggct tctcggacga accgagcatg ctcaccaacc
    24661 gcggagcgcc acattcttgt ggtatgaaat atcggctcag gggactggcc cgcttgcgaa
    24721 catctcagag aaattttgtc ttcacggtta ctctaccaca gtaaaccgaa aagtgtcatt
    24781 caaagttttg gttagttttt tcggtgatgc gaccttgttc aagtaacgct tgttctatgg
    24841 tctgctgcag catccgaata cgctgttcca tacttgccgc gtagtcacga gtctttgctt
    24901 tttcttgcgc taactcatag ctgatattca atgcggcaat gaagaccaac tgttcagtat
    24961 ttgtgactct agtgcgttct ttcagatctt gcaaccgttg gttcagatcg tccgctgcct
    25021 gattcaacgc atccctttgg tcaggcgggc agttcacacg cagtgaacgg ccaaaaattt
    25081 ggatatcgac gggttgtgca gacatgccac cttcctgctg attgactgcg ctgccttcgt
    25141 ctttagaccc atggtctgcg aaggggcgac actatagcta ccctgatgag aagagacaag
    25201 cccttttctg gtccaccagg gcccaaagtg gtagcatatc atgaatattc ctccctttga
    25261 cgacgaatgc ttatgtctat acagaacgaa atgcctggtt acaacgaaat gaaccagtat
    25321 ctgaaccaac aagggacggg tctgacccct gccgagatgc atggtttaat cagtgggatg
    25381 atatgtggcg gtaacgatga cagctcatgg ctgccgctac ttcacgacct gacgaacgaa
    25441 ggcatggctt tcggtcatga gctggcacag gcactgcgta aaatgcactc tgccaccagc
    25501 gatgccctgc aggatgacgg cttccttttt cagctttatc tgcctgatgg cgatgatgtc
    25561 agcgttttcg atcgggctga tgcactggca ggttgggtca atcacttcct gcttggtctt
    25621 ggcgttacgc aaccgaagct ggacaaagtg accggcgaaa ccggtgaagc catcgacgat
    25681 ctgcgtaaca tcgcgcagct gggctacgac gaagacgaag atcaggaaga gcttgaaatg
    25741 tcgcttgaag agatcatcga atacgttcgt gttgccgcgc tgttatgcca cgacaccttt
    25801 actcatccgc aaccgaccgc gccagaagta caaaaaccga ctctacacta aaaacaaaaa
    25861 acgtaaggag agtgttatga gtgagatatc ccggcaagag tttcagcgtc gccgtcaggc
    25921 cctggtggag caaatgcaac ccggcagcgc cgcgctgatt tttgctgcac cagaagtaac
    25981 acgtagcgcc gacagcgaat acccctatcg tcagaacagt gacttctggt acttcaccgg
    26041 ctttaacgaa ccggaagcgg tgctggtgct gattaaaagc gatgacactc ataaccacag
    26101 cgttctgttt aaccgcgttc gcgacctgac ggcggagatc tggtttggcc gtcgcttagg
    26161 ccaggatgcc gcgccagaga aactgggcgt tgaccgcgca ctggcattca gcgaaatcaa
    26221 tcagcaactt tatcaactac ttaacggcct ggatgtggtt taccatgccc agggcgaata
    26281 tgcatatgct gatgaaatcg tgaacagtgc gctggaaaaa ctgcgtaaag gttcgcggca
    26341 aaatctcacc gcaccggcaa cgatgatcga ctggcgtcct gttgttcatg aaatgcgcct
    26401 gttcaaatcg ccagaagaga ttgccgtact ccgccgcgcg ggagaaatca ccgccatggc
    26461 acatacacgg gcgatggaaa aatgccgtcc gggaatgttc gagtaccatc tggaaggcga
    26521 aattcaccac gaatttaacc gccacggtgc gcgctatcct tcctataaca ccattgtagg
    26581 cagcggtgaa aacggctgca ttctgcacta caccgaaaac gagagtgaac tgcgcgacgg
    26641 tgacctggtg ttgattgacg cgggttgtga atacaaaggt tacgcgggcg atattacccg
    26701 caccttcccg gtcaacggca aattcaccca ggcccagcgt gaaatctacg acattgtgct
    26761 ggagtctctc gaaaccagcc tgcgcctgta tcgtccggga acttccattc tggaagtcac
    26821 tggtgaagtg gtgcgcatca tggttagcgg cctggtaaaa ctcggcatcc tgaaaggtaa
    26881 tgttgatgaa ctgatcgctc agaacgccca tcgtcctttc tttatgcatg gccttagcca
    26941 ctggttagga ctggatgtcc atgacgttgg cgtttatggt caggatcgct cgcgcattct
    27001 ggaaccgggg atggtactga ccgtagagcc tggtctgtat atcgcgccgg atgcggatgt
    27061 gccagaacaa tatcgcggta tcggcattcg tattgaagac gacattgtga ttaccgaaac
    27121 cggcaacgaa aacctcaccg ccagcgtggt gaaaaagccg gaagaaatcg aagcgttgat
    27181 ggctgctgcg agaaagcaat gagcgtaatc atcgtcggtg gcggcatggc gggcgcgacg
    27241 ctggcgctgg ctatttcccg gttaagtcac ggggcgctgc cggtacattt gattgaagcg
    27301 actgcgccag agtcacatgc tcatccgggc tttgatggac gagcgatagc gctggcggcg
    27361 ggtacctgtc agcaactggc gcgcatcggc gtctggcaat ctctggctga ttgcgcaacc
    27421 gccatcacta ccgtgcatgt cagcgatcgt ggtcatgctg gattcgtcac cctcgccgca
    27481 gaagattacc aactggcggc gctggggcag gttgtcgaat tgcataatgt cgggcaacgg
    27541 ctgtttgcat tgctgcgaaa agcacctggc gtaatgctgc attgccccga tcgcgtagct
    27601 aacgttgccc gtactcagag tcatgttgaa gtgacgctgg agagcggcga gatgctgacg
    27661 ggccgcgtgc tgatagccgc tgatggcacc cattcagcgt tagccaccgc gtgcggcgtt
    27721 gattggcagc aggagcctta cgaacaactg gcagtgattg ccaacgttgc tacttctgtt
    27781 gcgcacgaag gccgcgcttt tgaacgcttc acgcaacatg gcccgctggc gatgttgccg
    27841 atgtctgacg gacgctgttc gctggtctgg tgtcatccac tggaacggcg cgaagaggtg
    27901 ctgtcgtgga gcgacgagaa gttttgccgt gaactccagt ctgcctttgg ctggcgactg
    27961 gggcaaatta cccacgccgg taaacgcagt gcttatccgc tggcattaac ccgcgccgcc
    28021 agaccgatta cccatcgcac tgtgctggtg ggcaatgcgg cgcaaactct gcacccgatt
    28081 gccgggcaag ggtttaacct cggtatgcga gatgtcatga gccttgcgga aaccctgact
    28141 caggcgcatg agcgcggaga agacatcggt gattacggca tattgtgccg ctatcagcag
    28201 cgtcgacaga gcgatcgcga agcaaccatc ggcgtcacgg acagcctggt acatcttttt
    28261 gccaaccgtt gggcaccgct ggttgtcggg cgcaacatcg gactgatgac gatggaatta
    28321 ttcaccccgg cacgcgatgt gctggcgcag cgcaccctcg gttgggtggc gcgttgaagg
    28381 cggttttaag gaataaacaa tgcaaagtgt tgatgtagcc attgttggcg gcggcatggt
    28441 ggggctggcg gttgcctgcg gcttacaggg gagcggctta cgcgttgccg tactggagca
    28501 gcgcgtaccg gaacctctgg cggtagatgc actaccacaa ctgcgcgttt cggctatcaa
    28561 tgccgccagc gaaaaattac tcacccgtct tggcgtctgg caggacattc tcgctcgtag
    28621 ggccagttgt tatcacggca tggaagtgtg ggacaaagac agttttggtc acatctcgtt
    28681 tgacgatcaa agcatgggct atagccatct tgggcacatc gttgaaaact cagtgattca
    28741 ctacgcgctg tggaacaaag cgcagcagtc gtcagatatt actctgttgg cccccacaga
    28801 attacagcag gtcgcctggg gagaaaatga aaccttcctg acgctgaaag atggcagcat
    28861 gttaacggcg cgtctggtga ttggcgcgga cggtgctaat tcctggttgc gcaacaaagc
    28921 agatattccg ctgactttct gggattatca gcatcacgcg ctggtagcga ccattcgcac
    28981 ggaagaaccg catgatgcgg tggcgcggca ggttttccat ggcgaaggca ttctggcctt
    29041 tttaccgctt agcgatccgc atctttgctc gattgtctgg tcactgtcgc cagaggaagc
    29101 gcagcggatg cagcaggcaa gtgaagacga atttaatcgc gcgttaaata tcgcttttga
    29161 taatcgcctt ggcttatgca aggttgagag cgcgcgtcag gtgttcccgc tgacggggcg
    29221 ttatgcgcgc cagtttgccg cacaccgtct ggcgctggtg ggcgacgccg cgcataccat
    29281 tcacccactg gcggggcagg gcgttaatct cggatttatg gacgccgcag agctgattgc
    29341 tgaactgaaa cggttgcatc gtcagggtaa agacatcggg cagtacattt acctgcgccg
    29401 ctatgaacgt agccgcaagc acagcgcggc gctgatgctg gctggtatgc agggttttcg
    29461 cgatctcttt tccggcgcta atccggcgaa aaaactgctg cgtgatattg gtttgaaact
    29521 ggccgacacg cttcctggcg ttaagccgca acttatccgc caggcaatgg gattaaacga
    29581 tttgcctgaa tggctgcgtt aaaaatttct cctctgttgt ttatttgata cccatcacac
    29641 tttcatctcc cggttttttc gccgggagat tttcctcatt tgaaataaac taatttcacc
    29701 tccgttttcg cattatattt tctaatacca tgattttttg atttagtgtt ttttgacatt
    29761 tttttagctc ttaatattgt cttattcaaa ttgactttct catcacatca tctttgtata
    29821 gaaattggtg tattttttgg ttttttattc tgtcgcgatt tttgcatttt ttaaccataa
    29881 gctaatgtga tgatcaattt taccttatgg ttaacagtct gtttcggtgg taagttcagg
    29941 caaaagagaa cgattgcgtt ggggaccggg agtggctccg atgctgggtt ttgtggtgat
    30001 aatttcacca tgaaaaagtt gtcagccccg cttattcaat gaggacaaga tggcacaaca
    30061 gactcctttg tacgaacaac acacgctttg cggcgctcgc atggtggatt tccacggctg
    30121 gatgatgccg ctgcattacg gttcgcaaat cgacgaacat catgcggtac gtaccgatgc
    30181 cggaatgttt gatgtgtcgc atatgaccat cgtcgatctc cgcggcagcc gcacccggga
    30241 gtttctgcgt tatctgctgg cgaacgatgt ggcgaagctc accaaaagcg gcaaagccct
    30301 ttactcgggg atgttgaatg cctctggcgg tgtgatagat gatctcatcg tctactactt
    30361 tactgaagat ttcttccgcc tcgttgttaa ctccgccacc cgcgaaaaag acctctcctg
    30421 gattacccaa cacgctgaac ctttcggcat cgaaattacc gttcgtgatg acctttccat
    30481 gattgccgtg caagggccga atgctcaggc aaaagctgcc acactgttta atgatgctca
    30541 gcgtcaggcg gtggaaggga tgaaaccgtt ctttggcgtg caggtgggcg atctgtttat
    30601 tgccaccact ggttataccg gtgaagcggg ctatgaaatt gcgctgccca atgaaaaagc
    30661 ggccgatttc tggcgtgcgc tggtggaagc gggcgttaag ccctgcggtc tgggggcgcg
    30721 tgacacgctg cgtctggaag cgggcatgaa tctttatggt caggagatgg acgaaactat
    30781 ttctccttta gccgccaaca tgggctggac tatcgcctgg gaaccgacag atcgtgactt
    30841 tatcggtcgt gaagccctgg aagtgcagcg tgagcatggt acagaaaaac tggttggtct
    30901 ggtgatgacc gaaaaaggcg tgctgcgtaa tgaactgccg gtacgcttta ccgatgcgca
    30961 gggcaaccag catgaaggca ttatcaccag cggtactttc tccccgacgc tgggttacag
    31021 cattgcgctg gcgcgcgtgc cggaaggtat tggcgaaacg gcgattgtgc aaattcgcaa
    31081 ccgtgaaatg ccggttaaag tgacaaaacc tgtttttgtg cgtaacggca aagccgtcgc
    31141 gtgatttact tttttggaga ttgattgatg agcaacgtac cagcagaact gaaatacagc
    31201 aaagaacacg aatggctgcg taaagaagcc gacggcactt acaccgttgg tattaccgaa
    31261 catgctcagg agctgttagg cgatatggtg tttgttgacc tgccggaagt gggcgcaacg
    31321 gttagcgcgg gcgatgactg cgcggttgcc gaatcggtaa aagcggcgtc agacatttat
    31381 gcgccagtaa gcggtgaaat cgtggcggta aacgacgcac tgagcgattc cccggaactg
    31441 gtgaacagcg aaccgtatgc aggcggctgg atctttaaaa tcaaagccag cgatgaaagc
    31501 gaactggaat cactgctgga tgcgaccgca tacgaagcat tgttagaaga tgagtaacgg
    31561 ctttattcct cttctgcggg agaggatcag ggtgaggaaa atttatgcct caccctcact
    31621 ctcttcgtaa ggagagaggt tcacaattca ctgcacgttt caggaaccat cgctcatgac
    31681 acagacgtta agccagcttg aaaacagcgg cgcttttatt gaacgccata tcggaccgga
    31741 cgccgcgcaa cagcaagaaa tgctgaatgc cgttggtgca caatcgttaa acgcgctgac
    31801 cggccagatt gtgccgaaag atattcagct tgcgacacca ccgcaggttg gcgcaccggc
    31861 gaccgaatac gccgcactgg cagaactcaa ggctattgcc agtcgcaata aacgcttcac
    31921 gtcttacatc ggcatgggtt acaccgccgt gcagctaccg ccggttatcc tgcgtaacat
    31981 gctggaaaat ccgggctggt ataccgcgta cactccgtat cagcctgaag tctcccaggg
    32041 ccgccttgaa gcactgctca acttccagca ggtaacgctg gatttgactg gactggatat
    32101 ggcctctgct tctcttctgg acgaggccac cgctgccgcc gaagcaatgg cgatggcgaa
    32161 acgcgtcagc aaactgaaaa atgccaaccg cttcttcgtg gcttccaatg tgcatccgca
    32221 aacgctggat gtggtccgta ctcgtgctga aacctttggt tttgaagtga ttgtcgatga
    32281 cgcgcaaaaa gtgctcgacc atcaggacgt cttcggcgtg ctgttacagc aggtaggcac
    32341 taccggtgaa attcacgact acactgcgct tattagcgaa ctgaaatcac gcaaaattgt
    32401 ggtcagcgtt gccgccgata ttatggcgct ggtgctgtta actgcgccgg gtaaacaggg
    32461 cgcggatatt gtttttggtt cggcgcaacg cttcggcgtg ccgatgggct acggtggccc
    32521 acacgcggca ttctttgcgg cgaaagatga atacaaacgc tcaatgccgg gccgtattat
    32581 cggtgtatcg aaagatgcag ctggcaatac cgcgctgcgc atggcgatgc agactcgcga
    32641 gcaacatatt cgtcgtgaga aagcgaactc caacatttgt acttcccagg tactgctggc
    32701 aaacatcgcc agcctgtatg ccgtttatca cggcccggtt ggcctgaaac gtatcgctaa
    32761 ccgcattcac cgtctgaccg atattctggc ggcgggcctg caacaaaaag gtctgaaact
    32821 gcgccatgcg cactatttcg acaccttgtg tgtggaagtg gccgacaaag cgggcgtact
    32881 gacgcgtgcc gaagcggctg aaatcaacct gcgtagcgat atcctgaacg ccgtggggat
    32941 cacccttgat gaaaccacca cgcgtgaaaa cgtaatgcag cttttcagtg tgctgctggg
    33001 cgataaccac gggctggaga tcgacacgct ggacaaagac gtggctcacg acagccgctc
    33061 tatccagcct gcgatgctgc gcgacgacga aatcctcacc catccggtgt ttaatcgcta
    33121 tcacagcgaa accgaaatga tgcgctatat gcactcgctg gagcgtaaag atctggcgct
    33181 gaatcaggcg atgatcccgc tgggttcctg caccatgaaa ctgaacgccg ccgccgagat
    33241 gatcccaatc acttggacgg aatttgccga actgcacccg ttttgcccgc cggaacaggc
    33301 tgaaggttat cagcagatga ttgcgcagct ggctgactgg ctggtgaaac tgaccggtta
    33361 cgacgccgtt tgtatgcagc cgaactccgg cgcacagggc gaatacgcgg gcctgctggc
    33421 gattcgtcat tatcatgaaa gccgcaacga agggcatcgc gatatctgcc tgatcccggc
    33481 ttctgcgcac ggaactaacc ccgcttctgc acatatggca ggaatgcagg tggtggttgt
    33541 ggcgtgtgat aaaaacggca acatcgatct gactgatctg cgcgcgaaag cggaacaggc
    33601 gggcgataac ctctcctgta tcatggtgac ttatccttct acccacggcg tgtatgaaga
    33661 aacgatccgt gaagtgtgtg aagtcgtgca tcagttcggc ggtcaggttt accttgatgg
    33721 cgcgaacatg aacgcccagg ttggcatcac ctcgccgggc tttattggcg cggacgtttc
    33781 gcacctcaac ctgcataaaa ctttctgcat tccgcacggc ggtggtggtc cgggtatggg
    33841 accgatcggt gtgaaagcgc atctggcacc gtttgtaccg ggtcatagcg tggtgcaaat
    33901 cgaaggcatg ttaacccgtc agggcgcagt ttctgcggca ccgttcggta gcgcctctat
    33961 cctgccaatc agctggatgt acatccgcat gatgggcgca gaagggctga aaaaagcaag
    34021 ccaggtggca atcctcaacg ccaactatat tgccagccgc ctgcaggatg ccttcccggt
    34081 gctgtatacc ggtcgcgacg gtcgcgtggc gcacgaatgt attctcgata ttcgcccgct
    34141 gaaagaagaa accggcatca gcgagctgga cattgccaag cgcctgatcg actacggttt
    34201 ccacgcgccg acgatgtcgt tcccggtggc gggtacgctg atggttgaac cgactgaatc
    34261 tgaaagcaaa gtggaactgg atcgctttat cgacgcgatg ctggctatcc gcgcagaaat
    34321 tgaccaggtg aaagccggtg tctggccgct ggaagataac ccgctggtga acgcgccgca
    34381 cattcagagc gaactggtcg ccgagtgggc gcatccgtac agccgtgaag ttgcggtatt
    34441 cccggcaggc gtggcagaca aatactggcc gacggtgaaa cgtctggatg atgtttacgg
    34501 cgaccgtaac ctgttctgct cctgcgtacc gattagcgaa taccagtaat tcactgattc
    34561 gagtattttc taaaggcgct tcggcgcctt tttagtcaga tgacaaagta caaaagtgct
    34621 cagacagtcc cctcgcccct ttggggagag ggttagggtg aggggaacag gccggcactg
    34681 gcgcgaatat ttaccctcac cccgaccctc tccctgaaag ggcgaggggg aaaagcgtgc
    34741 caacattgaa gattgagcca gtttgttagc aatctcaaag atacgtcaac gaattaattt
    34801 ttctcggaaa aacaaatggc tatagcactt gtgaccggtg gcagtcgcgg catcgggcgg
    34861 gcaactgcat tactgttggc gcaagaaggg tatacggtgg cggttaatta tcagcaaaac
    34921 ctccacgcgg cgcaggaagt gatgaactta ataatgcaag ccggtggcaa agcattcgtg
    34981 ctccaggcgg atatcagcga cgaaaaccag gtcgttgcga tgtttacagc aatcgatcag
    35041 cacgatgaac cgctagcagc gctggtcaat aacgccggga tcttgtttac ccagtgcacc
    35101 gttgaaaacc ttaccgcaga gcgaatcaac cgagtacttt ccaccaacgt gacgggatat
    35161 tttctctgct gccgcgaggc ggtaaaacgc atggcgctta aaaatggtgg cagtggcggc
    35221 gctatcgtca atgtctcttc ggtggcctca cggttgggtt cgccagggga atatgttgat
    35281 tacgcggcat cgaaaggggc gattgattcg ttaaccaccg gactatcgct ggaagtcgcc
    35341 gcgcagggga tccgcgttaa ctgcgtgcgg ccagggttta tttataccga aatgcacgcc
    35401 agcggcggcg agcctggacg cgtcgatcgc gttaagtcga acatccccat gcagcgtggt
    35461 ggacaggcag aagaggtcgc gcaggccatt gtctggctac taagtgataa agcctcttac
    35521 gtcacgggaa gttttatcga tttggcgggc gggaaataaa acagggaagt tgtctgaccg
    35581 gatgcaacaa gtattgcatc cggtacttca tcgacttaaa gcttctcgcc gttgctggca
    35641 atcacctctt tgtaccagtt aaagctcttc ttacgtgaac gcgacatatc gccagtaccg
    35701 tcgtcatgtt tattcacata gataaagccg tagcgtttgc tgtactgccc ggtagtgaat
    35761 gacacgcagt cgatgcagcc ccacggtgtg tagcccatca gatccacacc atcgtaagtc
    35821 actgcttttt tcatctcttc aatatgggcg cgcaggtagt caatgcggta gtcgtcgttg
    35881 atgctgccat cttcttccac tttgtcgtaa gcgccaaaac cgttttcgac aataaacagc
    35941 ggcctctgat aacgctcata cagttcgcaa agggcatagc gcagacctac tggatcaatc
    36001 tgccagcccc agtcagacgc tttaacatac gggttcggta cgctgccttc aaaaccagag
    36061 atcgcatcgc cggtgccgcc ttcggccttc actgcgttgg tcatgtaata gctgaaacca
    36121 agataatcgc aggtgccttc gcgcagtaca tcgagatcgc cgtcttccat tttgatatta
    36181 aatccgcgac gttcccactc gttcaataca taggacgggt aatagccgca tagctgcaca
    36241 tcggtaaaga cgtagcgttc gcgcatcgac tcctgagcga acatcacatc gtccgggtta
    36301 catgagtagg gatagagcgg caccatcgcc agcatacagc cgactttcat ctccgggtta
    36361 atgcgacgcg cggctttcac tgccagggcg ctggcgacaa actggtgatg cagcacctgg
    36421 tacatcgtct cttccgggtt gtcatgctcg gtatacacca cgccggagca gcagtaacca
    36481 aacagcggcg cacgccagtt acgctggttg tttatttcgt tgaaggtcat ccagtatttg
    36541 actttatgct tgtagcgttc gaataccact tccgcgaaac ggacaaagaa atcaacgaat
    36601 ttacggttgg tccagctacc gtattgctgc accagatgca gcggcatttc aaagtgggag
    36661 agggttatca ccggttcgat gttgtatttc agcagttcat cgaacagaga gtcgtaaaac
    36721 ttcagccctt cttcgtttgg ctgagcttca tcaccttttg gaaaaatacg cgtccaggcg
    36781 atggaagtcc gaaaacattt gaagcccatt tcggcaaata gcttgatgtc ttccttgtag
    36841 tgaccataaa aatcaacggc ttcatggttt ggatagtatt ttcctggcaa gacttctttg
    36901 gtgatttcgc gcggcacgcc gtgtgcgcca caggtcagaa cgtcacaaat gctcggtcct
    36961 ttgccgcctt tgttccagcc gccttcgacc tgatgagcgg caacggcgcc accccataag
    37021 aaatctttcg gtaaggtgag ttttttcact atcataggct ccagatcagt caattcttac
    37081 aggaaatagt accatgcagc caaacgacat cacttttttc aacgtttcca ggatgacatt
    37141 ctggctgggc gtaaaaccat caccatccgc gacgagtctg aatcgcactt caaaacgggt
    37201 gatgtgcttc gtgtcggacg ttttgaagat gacggttatt tttgcacgat tgaagtcacc
    37261 gcaacctcaa ccgtaacgct ggatacactg acggaaaaac atgcagagca ggaaaatatg
    37321 accctgcctg aactgaaaaa ggtcattgct gacatctatc ccggtcagac acaattttat
    37381 atgattgaat ttaaatgtct ttaaattgaa ctcacgcctg ttagttgaaa tttcgcattt
    37441 aatttcatga tttttaggag attagtctca aatcagaatt tattttagct aacaggtgtt
    37501 cactggaact attctcagtt acgctggaga ggtatacatg tgcgtgtacg tttctctgga
    37561 gtaagttatg gttcagaagc ccctcattaa gcagggatat tcgctggcag aggaaattgc
    37621 caacagcgtc agtcacggca ttgggctggt gtttggtatc gttgggctgg tgttgctact
    37681 ggttcaggcg gtggatctca atgccagcgc cacagcgata accagttaca gcctctatgg
    37741 cggcagtatg atcctgctgt tcctcgcttc gacgctctat cacgccattc ctcatcaacg
    37801 ggcaaaaatg tggctgaaga aatttgacca ttgcgctatt tacctgttga ttgccggaac
    37861 ctacacgccg tttttgctgg tggggctgga ttctccgtta gcgcgcgggt tgatgattgt
    37921 tatctggagc ctagcattgc tggggattct gttcaaactg accatcgcgc accgattcaa
    37981 aattttatct ctggtgactt atctggcgat gggctggctg tcgctggtgg taatttatga
    38041 aatggcagtt aagctcgcgg cgggcagcgt taccttactg gcggtaggcg gcgtggttta
    38101 ttcgctcggg gtgattttct acgtctgcaa acgcattcca tacaaccatg ccatctggca
    38161 cggcttcgtg ctcggcggta gtgtgtgcca ctttctggcg atctatttgt atattgggca
    38221 ggcgtaatgg gtatgccttt gtaggcctga taagacgcgt cagcgtcgca tcaggcatcg
    38281 gagcgcaacc gccggatgcg gcgtgaacgc cttatccgat ctacgggcct cgtccctgtt
    38341 gtgataaagg catcaaatcc cagacatgat aagacgcgtc agcgtcgcat caggcgataa
    38401 atgcctgatg cgatacagag atttactctt cgagcgaata cggcagcggc tcgatatgca
    38461 gtgtattcgc atcgtcgcgt acgcggaaga tgctgtccgg ttccatatcg ttattcatga
    38521 cgacctgtac cacgacctga ccatcttcca gttttaccgc agccagcacc gtaccggtac
    38581 gacgccagtt ctcgcccatt ttcagctcta agtcttcacc agcttccggc agtcggctgg
    38641 cactacctgc cagcaaccag agcgcgcgtt tgttggcacc acggaatttt gctcgcgcca
    38701 ccatctcttg tccggtataa cagcctttct taaagctgat accgcccagc gcctggagat
    38761 tggtcgcctg tgggataaac tgcccgctgt tggcggcatc aatcaccggg aaaccagctt
    38821 caatgtttaa tgccagccac tgttggctat tgttcagttc cgcttcaccg cgcagtttat
    38881 cggtcagcat attagcagta gcttcatcgg ttacgatcag gaaacgttct gccgggtgtt
    38941 caaaccatag caaagtggtc gcgccttctt tgactacctg tttttctttc gaaggcagtt
    39001 cgctaaagag atttgccagc gcggcgcgcg cctgaaaacc ggcaacacca agcagcacac
    39061 gctcgtcgtc tggcgcgatg gtcactttag agaataccgc atattttttc agttcagtca
    39121 gctgcggttc acgcacgctg cgccgttcaa tccatgcaaa gccatcgccg tcgcggaaca
    39181 gacgtaaatt gctccacatt ttacctttgg cgtcgcaatg ggcggcgagc aggtgctgat
    39241 cttctgccat ctggctgaca tctgctgtca cctgaccctg catatatttt tcgctgtccg
    39301 cgccagtaat ggtggcaagc gcccagtcat caagcgtcat cagcgtcagc ggtaaacggg
    39361 cagaagccgt aggctgacgt ggaggaaaag gtgtaaaagc cataatagag tcctgagttg
    39421 cttaacggcg tattaatgac taatggtaaa agagctatga ggcaatgcaa gtgctttcac
    39481 tacgcgatct gtgccttctg cgaggcgact gccaaaaaaa tgaagccctt gaattgttgt
    39541 gaattaattt cgcgatcgcg atggtgttta ctgatattcc cgtaaaagaa gtcgaaaaac
    39601 acgttacaat agccgggtac tggattttcg tgagacagga agaagatgga cattaacaac
    39661 aaagcccgaa ttcattgggc atgccgccgt ggtatgcgcg aactcgatat ttcaatcatg
    39721 ccgtttttcg aacatgagta cgacagctta agcgatgacg aaaaacgcat ctttattcgt
    39781 ctgctggaat gtgacgatcc ggacctgttt aactggctga tgaatcacgg taaaccggcc
    39841 gatgcagaac tggaaataat ggtccgactc atccagacac ggaaccggga acgtggtcct
    39901 gtggcaatct gatttgcgcg tctcctggcg cgcacagtgg ctttccttgc tgattcatgg
    39961 gctggttgcc gctgttattt tactcatgcc ctggccgctc agttacaccc cgttatggat
    40021 ggtgttactt tcgctggtgg tatttgattg cgttcgcagc cagcggtgta ttaatgctcg
    40081 ccagggggaa attcgcttgt tgatggacgg gcgtttgcgt tggcaagggc aggagtggag
    40141 catcgtcaaa gcaccgtgga tgattaagag cggcatgatg ctgcgtttac gttctgatgg
    40201 cggtaaacgg caacatttat ggctggcagc cgacagcatg gacgaagccg aatggcggga
    40261 tttacggcgg attttgttgc aacaagagac gcaaagataa agatgacggc aaagcatatg
    40321 cgctaagccg tcagtgagat caggcgtaat gctctgccat ttcgttgagg atttgctcgc
    40381 accagctctg aatacgctcg tcgctaaggt catactggtt agtttcatcc agcgccagac
    40441 ccacgaacag ttgcccgtca gcaatcaccg gtttcgggct ggtaaattca tatccttccg
    40501 ttggccagta gccgacgaac ttcacgcctt tggtcgagag tttgtcatgc agcataccga
    40561 gcgcatcgag gaaccactcg ccgtatccca gttgatcgcc aagcccatac agcgcaataa
    40621 ttttaccttc aaggttcagg tcgtcgagct gatcccagac ggcttcccag tcttcctgga
    40681 tttcaccaaa atcccaggtc gggataccca gaatgagcac atcgtactgc tccattaatt
    40741 tcggggagtc gtccttgagg ttatgtaagg tcaccagttc tgggccgata atatcgcgga
    40801 ttttttctgc cgccatttcg gtgtaacagg tgctggaacc gtaaaaaaga cccatattca
    40861 taacgtaagc ttctcgattc tgctgtggct tttgcagcta gtgtatcaga atcgctttag
    40921 tcacggcaca ataaggcata atggatcaaa tgagtgacca taaggggcgc aagtgaaaca
    40981 ggagctggca cgcatcgagc agtttcttga tgctctgtgg ctggaaaaaa atctggctga
    41041 aaatacgttg aacgcttacc gtcgcgatct gtcaatgatg gtggagtggt tgcatcaccg
    41101 cgggttgacg ctggcgacgg cgcaaagtga cgatttgcag gcattactgg cagaacggct
    41161 ggagggcggg tataaagcca ccagctcagc gcgtttgctg agtgcagtgc gccgattgtt
    41221 ccagtatctt tatcgcgaaa agtttcgtga agacgatccc agtgcgcatc tcgcttcacc
    41281 gaaattgccc cagcgtttgc caaaagattt aagcgaagcg caggtcgaac gtttattaca
    41341 ggcgccatta attgatcagc cactggagct acgcgataaa gccatgcttg aagtgttgta
    41401 tgctaccggg ctgcgtgtct ctgaactggt cgggctgaca atgagtgata tcagcctgcg
    41461 tcagggcgta gtgcgggtca ttggtaaagg caacaaagag cgcctggtgc cgttagggga
    41521 agaggcggtt tactggctgg aaacctatct ggaacatggg cgtccgtggc tgctgaatgg
    41581 tgtgtcaatt gacgtgttgt ttcccagcca gcgtgcgcag cagatgacgc gacagacctt
    41641 ctggcaccgc attaaacatt atgccgtgct ggcgggtatc gacagcgaaa agctgtcacc
    41701 gcatgtgttg cgtcacgctt ttgccactca tttattaaat catggtgcgg atttacgcgt
    41761 ggtgcagatg ctactgggcc acagcgatct ttccaccacg caaatttata cgcatgtcgc
    41821 taccgagcgt ctgcggcaac ttcatcaaca gcatcacccg cgggcgtgat gtctgaaaag
    41881 aacgggaaga tttatgaaga aaggttttat gttgtttact ttgttagcgg cgttttcagg
    41941 ctttgctcag gctgatgacg cggcaattca acaaacgtta gccaaaatgg gcatcaaaag
    42001 cagcgatatt cagcccgcgc ccgtagctgg catgaagaca gttctgacta acagcggtgt
    42061 gttgtacatc accgatgatg gtaaacatat cattcagggg ccaatgtatg acgttagtgg
    42121 cacggctccg gtcaatgtca ccaataagat gctgttaaag cagttgaatg cgcttgaaaa
    42181 agagatgatc gtttataaag cgccgcagga aaaacacgtc atcaccgtgt ttactgatat
    42241 tacctgtggt tactgccaca aactgcatga gcaaatggca gactataacg cgctggggat
    42301 caccgtgcgt tatcttgctt tcccgcgcca ggggctggac agcgatgcag agaaagaaat
    42361 gaaagctatc tggtgtgcga aagataaaaa caaagcgttt gatgatgtga tggcaggtaa
    42421 aagcgtcgca ccagccagct gcgacgtgga tattgccgac cattacgcac ttggcgtcca
    42481 gcttggcgtt agcggtactc cggcagttgt gctgagcaat ggcacacttg ttccgggtta
    42541 ccagccgccg aaagacatga aagaattcct cgacgaacac caaaaaatga ccagcggtaa
    42601 ataattcgcg tgaaacaaca gatacaactt cgtcgccgtg aagtcgatga aacggcagac
    42661 ttgcccgctg aattgcctcc cttgctgcgc cgtttatacg ccagccgggg agtacgcagt
    42721 gcgcaagaac tggaacgcag tgttaaaggt atgttgccct ggcagcaact aagcggtgtc
    42781 gaaaaggccg ttgagatcct ttacaacgct tttcgcgaag gaacgcggat tattgtggtc
    42841 ggtgatttcg acgccgacgg cgcgaccagc acggctctaa gcgtgctggc gatgcgctcg
    42901 cttggttgca gcaatatcga ctacctggta ccaaaccgtt tcgaagacgg ttacggctta
    42961 agcccggaag tggtcgatca ggcccatgcc cgtggcgcgc agttaattgt cacggtggat
    43021 aacggtattt cctcccatgc gggagttgag cacgctcgct cgttgggcat cccggttatt
    43081 gttaccgatc accatttgcc gggcgacaca ttacccgcag cggaagcgat cattaaccct
    43141 aacttgcgcg actgtaattt cccgtcgaaa tcactggcag gcgtgggtgt ggcgttttat
    43201 ctgatgctgg cgctgcgcac ctttttgcgc gatcagggct ggtttgatga gcgcggcatc
    43261 gcaattccta acctggcaga actgctggat ctggtagcac tggggacagt agcggacgtc
    43321 gtgccgctgg acgctaataa tcgcattctg acctggcagg ggatgagtcg catccgtgcc
    43381 ggaaagtgcc gtccagggat taaagcgctg ctggaagtgg caaatcgcga tgcacaaaaa
    43441 ctagccgcca gcgatttagg ttttgcgctg ggaccgcgtc tcaatgctgc cgggcgactg
    43501 gatgatatgt ccgttggtgt ggcgctgttg ctgtgcgaca acatcggcga agcgcgcgtg
    43561 ctggcaaatg aactcgatgc gctaaaccag acgcgaaaag agatcgaaca aggaatgcag
    43621 gttgaagccc tgaccctgtg cgagaaactg gagcgcagcc gtgacacgct acccggcggg
    43681 ctggcaatgt atcaccccga atggcatcag ggcgttgtcg gtattctggc ttcgcgtatc
    43741 aaagagcgtt ttcaccgtcc ggttatcgcc tttgcgccag caggtgacgg tacgctgaaa
    43801 ggttccggtc gctccattca ggggctgcat atgcgtgatg cgctggagcg attagacaca
    43861 ctctaccccg gcatgatgct caagtttggc ggtcatgcga tggcggcggg tttgtcgctg
    43921 gaagaggata aattcgaact ctttcaacaa cggtttggcg aactggttac tgagtggctg
    43981 gccccttcgc tattgcaagg cgaagtggtg tcagacggtc cgttaagccc ggccgaaatg
    44041 accatggaag tggcgcagct gctgcgcgat gctggcccgt gggggcagat gttcccggag
    44101 ccgctgtttg acggtcattt ccgtctgctg caacagcggc tggtgggcga acgtcatttg
    44161 aaggtgatgg tcgaaccggt cggcggcggt ccactgctgg atggtattgc ttttaatgtc
    44221 gataccgctc tctggccgga taacggcgtg cgcgaagtgc aactggctta taagctcgat
    44281 atcaacgagt ttcgcggcaa ccgcagcctg caaattatca tcgacaatat ctggccaatt
    44341 tagcgtcatc ttctctataa aaaagagcgt ggattgggta caatcccgct cttatcaccg
    44401 cattttgact agctcaataa aagaaatcag accatgtttg aaattaatcc ggtaaataat
    44461 cgcattcagg acctcacgga acgctccgac gttcttaggg ggtatctttg actacgacgc
    44521 caagaaagag cgtctggaag aagtaaacgc cgagctggaa cagccggatg tctggaacga
    44581 acccgaacgc gcacaggcgc tgggtaaaga gcgttcctcc ctcgaagccg ttgtcgacac
    44641 cctcgaccaa atgaaacagg ggctggaaga tgtttctggt ctgctggaac tggctgtaga
    44701 agctgacgac gaagaaacct ttaacgaagc cgttgctgaa ctcgacgccc tggaagaaaa
    44761 actggcgcag cttgagttcc gccgtatgtt ctctggcgaa tatgacagcg ccgactgtta
    44821 cctcgatatt caggcggggt ctggcggtac ggaagcacag gactgggcga gcatgcttga
    44881 gcgtatgtat ctgcgctggg cagaatcgcg tggtttcaaa actgaaatca tcgaagagtc
    44941 ggaaggtgaa gtggcgggta ttaaatccgt gacgatcaaa atctccggcg attacgctta
    45001 cggctggctg cgtacagaaa ctggcgttca ccgcctggtg cgtaaaagcc cgtttgactc
    45061 cggcggtcgt cgccacacgt cgttcagctc tgcgtttgtt tatccggaag ttgatgatga
    45121 tattgatatc gaaatcaacc cggcggatct gcgcattgac gtttatcgcg cgtccggcgc
    45181 gggcggtcag cacgttaacc gtaccgaatc tgcggtgcgt attacccaca tcccgaccgg
    45241 gatcgtgacc cagtgccaga acgaccgttc ccagcacaag aacaaagatc aggccatgaa
    45301 gcagatgaaa gcgaagcttt atgaactgga gatgcagaag aaaaatgccg agaaacaggc
    45361 gatggaagat aacaaatctg acatcggctg gggcagccag attcgttctt atgtccttga
    45421 tgactcccgc attaaagatc tgcgcaccgg ggtagaaacc cgcaacacgc aggccgtgct
    45481 ggacggcagc ctggatcaat ttatcgaagc aagtttgaaa gcagggttat gaggaaccaa
    45541 catgtctgaa caacacgcac agggcgctga cgcggtagtc gatcttaaca atgaactgaa
    45601 aacgcgtcgt gagaagctgg cgaacctgcg tgagcaggga attgccttcc cgaacgattt
    45661 ccgtcgcgat catacctctg accaattgca cgcagaattc gacggtaaag agaacgaaga
    45721 actggaagcg ctgaacatcg aagtcgccgt tgctggccgc atgatgaccc gccgtattat
    45781 gggtaaagcg tctttcgtta ccctgcagga tgtgggtggc cgcattcagc tgtacgttgc
    45841 ccgtgacgat ctgccggaag gcgtttacaa cgagcagttc aaaaaatggg acctcggcga
    45901 catcctcggc gcgaaaggta aactgttcaa aaccaaaacc ggcgaactgt ctatccactg
    45961 taccgagctg cgtctgctga ccaaagcact gcgtccgctg ccggataaat tccacggctt
    46021 gcaggatcag gaagcgcgct atcgtcagcg ttatctggat ctcatctcta acgatgaatc
    46081 ccgtaacacc tttaaagtac gctcgcagat cctctctggt attcgccagt tcatggtgaa
    46141 ccgcggcttt atggaagttg aaacgccgat gatgcaggtg atcccaggcg gtgccgctgc
    46201 gcgtccgttt atcacccacc ataacgcgct ggatctcgac atgtacctgc gtatcgcgcc
    46261 ggaactgtac ctcaagcgtc tggtggtcgg tggcttcgag cgtgtattcg aaatcaaccg
    46321 taacttccgt aacgaaggta tttccgtacg tcataaccca gagttcacca tgatggaact
    46381 ctacatggct tacgcagatt acaaagatct gatcgagctg accgaatcgc tgttccgtac
    46441 tctggcacag gatattctcg gtaagacgga agtgacctac ggcgacgtga cgctggactt
    46501 cggtaaaccg ttcgaaaaac tgaccatgcg tgaagcgatc aagaaatatc gcccggaaac
    46561 cgacatggcg gatctggata acttcgactc tgcgaaagcg attgctgaat ctatcggtat
    46621 ccacgttgag aagagctggg gtctgggccg tatcgttacc gagatcttcg aagaagtggc
    46681 agaagcacat ctgattcagc cgaccttcat tactgaatat ccggcagaag tttctccgct
    46741 ggcgcgtcgt aacgacatta acccggaaat cacagaccgc tttgagttct tcatcggtgg
    46801 tcgtgaaatc ggtaacggct ttagcgagct gaatgacgcg gaagatcagg cgcaacgctt
    46861 cctggatcag gttgccgcga aagacgcagg tgacgacgaa gcgatgttct acgatgaaga
    46921 ttacgttacc gcactggaac atggcttacc gccgacagca ggtctgggga ttggcatcga
    46981 ccgtatggta atgctgttca ccaacagcca taccatccgc gacgttattc tgttcccggc
    47041 gatgcgtccg gtgaaataag cattacgtta tgctcacaac cccggcaaat gtcggggttt
    47101 tttatttaag ctgggtaaat gcagataatc gtattctggc ttcgcgattt gtcgcctgca
    47161 tcaccatcca cggactgaac gcccacggcg tggcatcaat accgcgtaat acatcagcta
    47221 aatcacacca ttgataatcc atcacttcat catcgttgat ctgtaacgca ctggtggtgc
    47281 gtgcggcaaa taccggacac acttcatttt ccacaatgcc tctcggatcg gtggcgcggt
    47341 agcgaaagtc aggatagata gattcaggag gcgtaatttc cacgccaagc tcataacggc
    47401 aacggcggat caccgcgtct tcgctgcttt ctcccagttg tgggtgccca caaaccgagt
    47461 tagtccacac gccaggccac gcttttttgc tcagtgcgcg gcgggtaact aataattgcc
    47521 ccttggcatt aaacagccaa ctggagaacg cgagatgtaa gagggtgtct gccgtgtgtg
    47581 cggcatactt ttccagcgta cccgtgggaa ctccctgtgc attcaataaa atgacgtgtt
    47641 ccgtttgcat aatttctcac atgtaattct gatcactcat cattatagat aattgaagag
    47701 tgaaatgatt aaccaagaga gcgtttatcg acaaccgccc gttttgagcg gatgccgaca
    47761 ggtaaaaacc atttaaaaaa gtagcatcat atatttgaag cgactttcaa tggccattgc
    47821 aacacagtca gaaatgacag gaatagagag agtaaaaagc aacaacagac tgaatatagc
    47881 gcgcattaaa aaattcatat tattctccat gctcattttt cttcagcttg cgcatcagaa
    47941 gccacatacg tactgtcctg acttctaaat ctttatcaga gacggattcc ttcaccaggt
    48001 cagcatgatg ctgatagcca ttaaagaaga cgttcagcac aacggcgcta agggttgcga
    48061 gcataatgcc gctatgtagc agcggttgca gtacggccgg taacttagaa aagaaatcgt
    48121 gagagagcgt cggagtcatg ccaacgccga gactgatcgc cacaatatag aggttgtaac
    48181 ggttggtggt gtagttacag cgcgacagaa ttcgaatccc tgtcgccagt accatgccga
    48241 acatcactag accagcgccg cccagcacaa attgcggaat ggaggctacc agcaccgcca
    48301 tttttggcac catgccaaac aggattaata taattcccga agaaatacac acccagcggc
    48361 tatgaacgcg catcacgcta accaggccaa cgttttgaga aaatgacgtg tgggggaagc
    48421 tgttaaacgt gccgcctatc attgtcccta cgccatcgac acgcagcccg caaataatat
    48481 cgtgtgaaga gagtttacga ccgactattt cacccagtgc caggaacatc cccatcgact
    48541 cgataaacac gatgattaac acggcagtca tggtcagaat ggaaacggga tcgaaaatcg
    48601 gcataccaaa cgacatcggg gtgacaatcg caaaccacga ggcatcatgt agcccggata
    48661 aattgacttc attcatcatc cacgaaagta aaaagccaaa tacaatcccc agtaatacgg
    48721 cgacgttgga cataaatcct ttcgcatagc gagtaatgag caagataaaa attaagacgg
    48781 caaaggagat acctaaataa acgggattac catattgcgg attcccttta cctccagcgg
    48841 cccagtcaat acccacctga atgatgctaa gcccaataga agtaataacc acaccggtaa
    48901 ccagtggcgg gaataaaggc atcaagcgac caataagtgg cgctaataat gtggtgataa
    48961 aacccgcggc gatagtggca ccaaatatcc ccagcaggcc gatatccggg ttcatcccaa
    49021 tggctatcat tggtgttaca gcagcaaagg tcaccgacat aatcaccggc aggcggatcc
    49081 ccataaagcg gccgataccg atacattgca ataatgtgac gatcccgcag caaaagagat
    49141 ccgagctaat gagcatcgca atagcatctt ttgagaggcc cagtcggtca ccaatcataa
    49201 gaggaacagc gactgcacct gcgtacatga caaggacgtg ttgcagaccg agtatgatca
    49261 gctttcctgg tgataatatg cggtcaacct catcagttgg gcggtcttgc ccagaagatg
    49321 agggaagttg ggaatctatg gcgctcatca gtgtttctcc tttcgcccgc cagaataaaa
    49381 agcaaccgtt tacgttaatg aaactgtcta aacacatgct cgtggagtgc cagtttttgc
    49441 gaggaagggg agttagagac aggaattgcg gaggcaatca cataatcgtg cttaaaccac
    49501 gaaaatgaca agagaaactg acaatggaat tatctgtaat tttctcatat tgatatttca
    49561 ttaatcattc tggcatgtcg cactctcgca tttaatcgtt tttatctgga tagcgctctt
    49621 ttgatcggca atgttgaaat aatgaaagcg gcatagattg aggggcaata atttgaactc
    49681 agtcgcaaaa ttataattaa aacggtcatg ctacctaata tatgcccgtt tattgcatcg
    49741 cgaaatttga gaagacataa ctcattaaat gaaagggatg caaatgaata cgtttatcgc
    49801 tgctgaagct gcggaatgta taggctgtca
//
LOCUS       DAEBXN010000020        49739 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.020, whole genome shotgun sequence.
ACCESSION   DAEBXN010000020 DAEBXN010000000
VERSION     DAEBXN010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49739
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.020"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..185
                     /locus_tag="KJL99_001140"
     CDS             <1..185
                     /locus_tag="KJL99_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBD4435941.1"
                     /translation="SRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVE
                     LHDKVIGHYLNIKHYQ"
     gene            complement(179..1357)
                     /gene="setB"
                     /locus_tag="KJL99_001141"
     CDS             complement(179..1357)
                     /gene="setB"
                     /locus_tag="KJL99_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HBD4435942.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFWVMTPTY
                     "
     gene            1725..2855
                     /gene="fruB"
                     /locus_tag="KJL99_001142"
     CDS             1725..2855
                     /gene="fruB"
                     /locus_tag="KJL99_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HBD4435943.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDKHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            2855..3793
                     /gene="fruK"
                     /locus_tag="KJL99_001143"
     CDS             2855..3793
                     /gene="fruK"
                     /locus_tag="KJL99_001143"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBD4435944.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            3810..5501
                     /gene="fruA"
                     /locus_tag="KJL99_001144"
     CDS             3810..5501
                     /gene="fruA"
                     /locus_tag="KJL99_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HBD4435945.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTSQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            5925..6866
                     /locus_tag="KJL99_001145"
     CDS             5925..6866
                     /locus_tag="KJL99_001145"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HBD4435946.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQNHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            6854..7792
                     /gene="psuG"
                     /locus_tag="KJL99_001146"
     CDS             6854..7792
                     /gene="psuG"
                     /locus_tag="KJL99_001146"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HBD4435947.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEKIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTSGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            7886..9136
                     /locus_tag="KJL99_001147"
     CDS             7886..9136
                     /locus_tag="KJL99_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HBD4435948.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRVNRNELFTAICSGMASIAGSVMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(9207..9901)
                     /gene="yeiL"
                     /locus_tag="KJL99_001148"
                     /pseudo
     CDS             complement(9207..9901)
                     /gene="yeiL"
                     /locus_tag="KJL99_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
     gene            10032..10973
                     /gene="rihB"
                     /locus_tag="KJL99_001149"
     CDS             10032..10973
                     /gene="rihB"
                     /locus_tag="KJL99_001149"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HBD4435949.1"
                     /translation="MEKRKIILDCEPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERVGGPAGELFSD
                     IMNFTLKTQFEYYGLAGGPVHDATCIGYLINPDGIKTQDMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            11073..12323
                     /locus_tag="KJL99_001150"
     CDS             11073..12323
                     /locus_tag="KJL99_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HBD4435950.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEADATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(12379..13467)
                     /locus_tag="KJL99_001151"
     CDS             complement(12379..13467)
                     /locus_tag="KJL99_001151"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBD4435951.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKDKGYILTEQEYCVVVGTINMDIRGMADIHYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTVPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(13470..14327)
                     /gene="nfo"
                     /locus_tag="KJL99_001152"
     CDS             complement(13470..14327)
                     /gene="nfo"
                     /locus_tag="KJL99_001152"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HBD4435952.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQNDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(14401..15450)
                     /locus_tag="KJL99_001153"
     CDS             complement(14401..15450)
                     /locus_tag="KJL99_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HBD4435953.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            15549..16430
                     /locus_tag="KJL99_001154"
     CDS             15549..16430
                     /locus_tag="KJL99_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBD4435954.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTRDIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            16636..18105
                     /gene="lysP"
                     /locus_tag="KJL99_001155"
     CDS             16636..18105
                     /gene="lysP"
                     /locus_tag="KJL99_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HBD4435955.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            18397..20388
                     /gene="cirA"
                     /locus_tag="KJL99_001156"
                     /pseudo
     CDS             18397..20388
                     /gene="cirA"
                     /locus_tag="KJL99_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(20420..21255)
                     /gene="yeiG"
                     /locus_tag="KJL99_001157"
                     /pseudo
     CDS             complement(20420..21255)
                     /gene="yeiG"
                     /locus_tag="KJL99_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
     gene            21513..22181
                     /gene="folE"
                     /locus_tag="KJL99_001158"
     CDS             21513..22181
                     /gene="folE"
                     /locus_tag="KJL99_001158"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HBD4435956.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            22198..23274
                     /locus_tag="KJL99_001159"
     CDS             22198..23274
                     /locus_tag="KJL99_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HBD4435957.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYDAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLGVLLLLGLISDSQTSRAWTPDASAILYEKYWKLHGGVEAISNRA
                     DGVGNSLLALGAQYGWQLAGMMLIGAALMRSGWLKGQFSLRHYRRTGFVLVAIGVTIN
                     LPAIALQWQLDWAYRWCAFLLQMPRELSAPFQAIGYASLFYGFWPQLSRFKLVLAIAC
                     VGRMALTNYLLQTLICTTLFYHLGLFMHFDRLELLAFVIPVWLANILFSVIWLRYFRQ
                     GPVEWLWRQLTLRAAGPAISKTSR"
     gene            23416..24456
                     /gene="galS"
                     /locus_tag="KJL99_001160"
     CDS             23416..24456
                     /gene="galS"
                     /locus_tag="KJL99_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HBD4435958.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            24736..25734
                     /gene="mglB"
                     /locus_tag="KJL99_001161"
     CDS             24736..25734
                     /gene="mglB"
                     /locus_tag="KJL99_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HBD4435959.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            25795..27315
                     /gene="mglA"
                     /locus_tag="KJL99_001162"
     CDS             25795..27315
                     /gene="mglA"
                     /locus_tag="KJL99_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HBD4435960.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            27331..28341
                     /gene="mglC"
                     /locus_tag="KJL99_001163"
     CDS             27331..28341
                     /gene="mglC"
                     /locus_tag="KJL99_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HBD4435961.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKISGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(28377..29590)
                     /gene="preA"
                     /locus_tag="KJL99_001164"
                     /pseudo
     CDS             complement(28377..29590)
                     /gene="preA"
                     /locus_tag="KJL99_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
     gene            complement(29584..30822)
                     /gene="preT"
                     /locus_tag="KJL99_001165"
     CDS             complement(29584..30822)
                     /gene="preT"
                     /locus_tag="KJL99_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HBD4435962.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRVRQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDAFAELEQQRNTIKTQNYQIRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(31016..31255)
                     /locus_tag="KJL99_001166"
     CDS             complement(31016..31255)
                     /locus_tag="KJL99_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HBD4435963.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(31258..31977)
                     /gene="sanA"
                     /locus_tag="KJL99_001167"
     CDS             complement(31258..31977)
                     /gene="sanA"
                     /locus_tag="KJL99_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HBD4435964.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(32127..33011)
                     /gene="cdd"
                     /locus_tag="KJL99_001168"
     CDS             complement(32127..33011)
                     /gene="cdd"
                     /locus_tag="KJL99_001168"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBD4435965.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(33141..33836)
                     /locus_tag="KJL99_001169"
     CDS             complement(33141..33836)
                     /locus_tag="KJL99_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HBD4435966.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(33833..34231)
                     /locus_tag="KJL99_001170"
     CDS             complement(33833..34231)
                     /locus_tag="KJL99_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HBD4435967.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            34470..35417
                     /gene="dusC"
                     /locus_tag="KJL99_001171"
     CDS             34470..35417
                     /gene="dusC"
                     /locus_tag="KJL99_001171"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HBD4435968.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGFWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            complement(35788..35871)
                     /gene="yohP"
                     /locus_tag="KJL99_001172"
     CDS             complement(35788..35871)
                     /gene="yohP"
                     /locus_tag="KJL99_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HBD4435969.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            36095..37531
                     /gene="mdtQ"
                     /locus_tag="KJL99_001173"
                     /pseudo
     CDS             36095..37531
                     /gene="mdtQ"
                     /locus_tag="KJL99_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
     gene            37584..38345
                     /locus_tag="KJL99_001174"
                     /pseudo
     CDS             37584..38345
                     /locus_tag="KJL99_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(38462..39040)
                     /locus_tag="KJL99_001175"
     CDS             complement(38462..39040)
                     /locus_tag="KJL99_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBD4435970.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            39210..39797
                     /locus_tag="KJL99_001176"
     CDS             39210..39797
                     /locus_tag="KJL99_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HBD4435971.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLTLTVILWGYGYRLF"
     gene            39971..40903
                     /gene="pbpG"
                     /locus_tag="KJL99_001177"
     CDS             39971..40903
                     /gene="pbpG"
                     /locus_tag="KJL99_001177"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HBD4435972.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(40941..42656)
                     /gene="dld"
                     /locus_tag="KJL99_001178"
     CDS             complement(40941..42656)
                     /gene="dld"
                     /locus_tag="KJL99_001178"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HBD4435973.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARIDEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            42852..45149
                     /gene="bglX"
                     /locus_tag="KJL99_001179"
     CDS             42852..45149
                     /gene="bglX"
                     /locus_tag="KJL99_001179"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HBD4435974.1"
                     /translation="MKWLCSVGIAVSLVLQPALADDLFGNHPLTPEARDAFVTDLLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQGQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPISFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            45401..46318
                     /gene="osmF"
                     /locus_tag="KJL99_001180"
     CDS             45401..46318
                     /gene="osmF"
                     /locus_tag="KJL99_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HBD4435975.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVGRGAITSDELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPTFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            46325..47482
                     /gene="yehY"
                     /locus_tag="KJL99_001181"
     CDS             46325..47482
                     /gene="yehY"
                     /locus_tag="KJL99_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HBD4435976.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGLHLWQL
                     WPQTIWMLVGVGCAWLTACFVPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGRGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            47475..48401
                     /gene="yehX"
                     /locus_tag="KJL99_001182"
     CDS             47475..48401
                     /gene="yehX"
                     /locus_tag="KJL99_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HBD4435977.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVHLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            48406..49137
                     /gene="yehW"
                     /locus_tag="KJL99_001183"
     CDS             48406..49137
                     /gene="yehW"
                     /locus_tag="KJL99_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HBD4435978.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPILQVTLAGLGAIDPSVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(49118..49225)
                     /locus_tag="KJL99_001184"
     CDS             complement(49118..49225)
                     /locus_tag="KJL99_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HBD4435979.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(49285..49739)
                     /locus_tag="KJL99_001185"
                     /pseudo
     CDS             complement(49285..49739)
                     /locus_tag="KJL99_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
BASE COUNT        12023 a        12253 c        13360 g        12103 t
ORIGIN      
        1 aatcacgcct gaagggaaag ctgcacgtta tcagcaagcg ttacactcag cgcattgaac
       61 gacataatct gaatctgaga caacatctgg caaggctggg acggaagtca ctgtcgttct
      121 caaaatcggt ggagctgcat gacaaggtca tcgggcatta tctgaacata aaacactatc
      181 agtaagttgg agtcattacc caaaacagag tggcgataat catcaccatc gcaaaccaga
      241 acacagcgtg ataattccag atctcggcga cgatgcccgc cactgatcct gcgatgatcc
      301 agcccacgcg cgacgtgttg gtatagagcg tggtggctga acctgcctga ccgggcatca
      361 aatcctgaaa atagagcatc ccgatgccgc ccagaatgcc aataaaaata gcatttagca
      421 gctgcaagcc caacagaatg gcaggtgaat gcgccatcag cattcctgcg taaaaacaga
      481 cgccacccac ggcagcaacg cgcattaaga aacgcttacc cagacgtttg gcgaaatatc
      541 cggcaatcaa catcgtcggg atttccagcc cggcggcggt ccccatcatc acaccggcca
      601 gtttctcggg aagatgcagt tcgttgataa taaatagcgg catgttgatg atgtacaggc
      661 tgttcgagcc ccacatcaat gtacaaatga caaacagcag tagcgtatcg cgacggttac
      721 ggcgcggcgc ttcaaccgtg ccggtcgcca gcggaagctc tttttgcatc gacggtaaaa
      781 acagccacac catcacgccg caaacaataa acgctaccgc tgcgctcaga tacattaccg
      841 taaagctgaa acccatcgct aacgcataag ccagcggtgg gccaatgacc catgccagtg
      901 aaacctgagc gcgtaaaaaa gagctgaaca tcaccgcctc acgtccggtt ttgtcggcat
      961 gttcacgggc aagggcaaac atttgcgggt tagcggtcga gccaaagctg ctaagaaaga
     1021 cgccaacgaa tagcaaaaca aagtagttgc gattccaagc aaaaagggtg caggccagca
     1081 cgcctaacag gcagcaaaag acaatcagcg atttgcgatc gccgcgctta tcagagcgcc
     1141 cagcgagaaa ctgacttacc agaatcccaa tgacagcgct gccggtaaag aagaatccca
     1201 ccatcgccgg acgggcatgt acttcatcgg taaggaaaat actgagtgtc ggggtttgca
     1261 gagcacccgc aataccggtg agaaaggcaa cgattaaaaa cgccgtcgag gtcaggtcaa
     1321 acgatttcgc gctggagact gcgggggagt tatgcatgct ggtcgatcac tggaagagga
     1381 agacgcggag tttacgctgc ttgtcagaaa ataaacagac agcgaaatca aaagcgccac
     1441 gaataatcaa aatgccattt cactgttgct gattggctgc tgacgctgct gaaatgaaat
     1501 tgctgataac gaattttccc atcagcaatt aggaaaaatg acaaaaaatt gtgcagcaca
     1561 tcaaactttt gctcataact ttacggcttt ccttgcgtgc tgaaacagaa gtattatgct
     1621 ttcttgaaac gtttcagcgc gatcttgtct ttaaccctaa gctaggttgg cgcttttttt
     1681 ctcatagagg ctgaatcgtt tcaattcagc aagagaggag aactatgttc cagttatccg
     1741 tacaggacat ccatccgggc gaaaaggccg gagacaaaga agaggcgatt cgccaggtcg
     1801 ctgcggcgct ggtgcaggcc ggtaatgtag cagaaggcta cgtcaatggc atgctggcgc
     1861 gcgaacagca aacctcaacg ttcctcggca atggtattgc tattccacac ggcactaccg
     1921 acacccgcga tcaggtgctg aaaaccggcg ttcaggtatt tcagttcccg gaaggcgtca
     1981 cctggggtga cggtcaggta gcgtacgtgg caatcggtat tgctgccagc tcggataaac
     2041 atctgggcct gctacgccag ctgacccacg tactgagcga tgattccgtt gctgaacaac
     2101 tgaagtcagc aacaacagca gaagaacttc gcgcattact gatgggcgaa aagcagagtg
     2161 agcagctgaa gctcgacaac gaaatgctga cgctggatat cgtcgccagc gatctgctga
     2221 ctcttcaggc gctgaacgct gcgcgtctga aagaggcgag ggcagttgac gccactttcg
     2281 tcaccaaagc catcaatgaa caaccgctga atctcggaca gggtatctgg ctgagcgata
     2341 gcgccgaagg caatctgcgt agtgcgattg cggtaagccg tgcggcaaat gcttttgatg
     2401 tggacggcga aacggcagcc atgctggtga gtgtggcgat gaatgacgat cagcccatcg
     2461 cggttcttaa gcgtctcgct gatttgttgc tcgacaataa agctgaccgc ttgctgaaag
     2521 cggatgcggc aacgttgctg gcgctgctga ccagcgatga tgcgccgacc gacgacgtgt
     2581 taagcgcgga gtttgtggtg cgcaatgaac acggcctgca tgctcgtcca ggtaccatgc
     2641 tggtcaatac cattaaacaa tttaacagtg atattaccgt gacaaacctt gatggcaccg
     2701 gcaaaccggc aaacggacgt agtctgatga aagttgtggc acttggcgtt aagaaaggtc
     2761 atcgcctacg ctttaccgcc cagggtgcag atgctgaaca ggcgctgaaa gccattggcg
     2821 acgctatcgc tgctggtctt ggggagggcg cataatgagc agacgtgttg ctacgatcac
     2881 ccttaatccg gcttatgacc ttgttggttt ctgcccggaa attgaacgcg gcgaagtgaa
     2941 cctggtgaaa accaccggtc tgcatgcggc gggtaaaggc atcaacgtgg ccaaagtatt
     3001 aaaagacctg ggaattgatg tcaccgttgg cggcttcctg ggtaaagaca atcaggatgg
     3061 ttttcagcaa ctgttcagcg agctgggcat tgccaaccgt ttccaggttg tacaggggcg
     3121 cacccgaatt aacgttaagc tgacggaaaa agacggcgaa gtgaccgact tcaacttctc
     3181 gggtttcgaa gtcacccccg ccgactggga acgctttgtg actgattctc tgagctggct
     3241 cggtcagttc gatatggtct gtgtcagcgg aagcttaccg tcaggcgtca gcccggaagc
     3301 gttcaccgac tggatgactc gcctgcgtag tcagtgtcct tgcattatct ttgatagtag
     3361 ccgtgaagcg ttagtagcag gtttgaaagc ggcaccgtgg ctggtgaaac ctaaccgccg
     3421 cgagttggaa atctgggcag gccgtaaact gcctgaaatg aaagatgtga ttgaagctgc
     3481 acatgcgcta cgtgaacaag gtattgcgca tgttgttatt tcactgggtg ccgaaggcgc
     3541 gctttgggtt aatgcctccg gcgaatggat cgctaaacca ccgtcagtcg atgtcgtaag
     3601 caccgttggc gcaggggatt ctatggttgg tggcctgatt tatggcttgc tgatgcgtga
     3661 atccagtgaa cacacactgc gtctggcgac agctgttgca gccctggcgg taagtcaaag
     3721 caatgtgggt attaccgatc gtccgcagtt ggccgcaatg atggcgcgcg tcgacttaca
     3781 accttttaac tgacagcagg agaggcataa tgaaaacgct gctgattatt gacgctaatc
     3841 tcggtcaggc acgcgcctat atggcgaaga ccctgctggg cgcggcggcg cgaaaagcaa
     3901 aactggaaat catcgacaat ccgaacgacg cagaaatggc gattgttctc ggtgattcca
     3961 tcccgaatga cagcgcgctg aacggtaaaa atgtctggct gggtgatatt tcccgggcag
     4021 ttgcgcaccc tgagctgttc ctgagtgaag ccaaaggcca tgcgaaacct tacactgcgc
     4081 cggtcactgc gacagcaccg gttgccgcca gcggtccgaa acgcgtagtt gcggtgactg
     4141 cttgcccgac tggcgtagca cacaccttta tggcggctga agccattgaa accgaagcga
     4201 aaaaacgtgg ctggtgggtg aaagttgaaa cccgtggttc tgttggcgcg ggtaatgcaa
     4261 tcactcccga ggaagtagcc gcagcggatc tggtgattgt ggcggcagat atcgaagtgg
     4321 atctggcgaa atttgctggt aaaccgatgt atcgtacctc taccggtctg gcgctgaaga
     4381 aaacctcgca ggaactggat aaagcggttg ctgaagcaac gccgtatgaa ccggcgggca
     4441 aagctcaaac ggcgaccact gaaggtaaga aagagagtgc aggcgcttat cgtcacttgc
     4501 taacgggcgt ctcttacatg ctgccgatgg tcgttgctgg tggtctgtgt atcgcgcttt
     4561 cttttgcttt tggtatcgaa gcgtttaaag agccgggtac gttggcagcg gcgctgatgc
     4621 agattggtgg tggttcagcc tttgcgctga tggtgccggt actggcaggt tatattgcct
     4681 tctccattgc cgatcgtccg ggcctcacgc cgggtctgat tggcggtatg ctggcggtca
     4741 gcaccggttc tggcttcatt ggcggtatta ttgcgggctt cctggctggt tacattgcga
     4801 agttaatcag tacgcaactg aaactgccgc agagtatgga ggcgctgaaa ccgatcctga
     4861 tcattccgct aatttccagt ctggtggtcg gtctggcgat gatctacctg atcggtaaac
     4921 cagttgctgg cattctcgaa gggctgactc actggctgca gaccatgggg actgcgaatg
     4981 cggttctgct gggggcgatc ctcggtggca tgatgtgtac tgacatgggc ggtccggtaa
     5041 acaaagcagc gtacgcattc ggtgtgggtc tgctgagtac tcaaacctat ggcccgatgg
     5101 cggcgattat ggcggcaggt atggtgccac cgctggcaat gggcctggca acaatggtgg
     5161 cgcgtcgcaa attcgacaaa gcgcagcagg aaggtggcaa agccgctctg gtattgggac
     5221 tgtgcttcat ttcggaaggt gcaattccgt ttgctgctcg tgatccgatg cgtgtgctgc
     5281 cgtgctgtat cgtgggtggg gcgctgactg gcgcaatctc aatggcgatt ggtgcgaaac
     5341 tgatggcacc gcacggtggt ctgtttgttc tgctgatccc tggcgctatt acgccggtat
     5401 tgggttacct ggtagcaatt attgccggta cgctggtggc gggtttggcc tatgccttcc
     5461 tgaaacgtcc ggaagtggac gcagtagcga aagcagcgta ataaaaggtg tgttacaggg
     5521 cagaaatttc ctgccctgta acatttgatg agcgcaaaga gaatgaccag cttgtgaatg
     5581 taattttatg aatttaaatg gttttatctt tcagtagaaa cttcaaacgt tcatcaaaca
     5641 ctaaggttat ttcctcaagg gcagattatt ttctgccctt ttttagtgtt cctgttctgt
     5701 gcatcccgtc acaaattcct cgcttttgta attcaggcat ttaatcgcat tttgtgagcc
     5761 tgctcaaatc tgcccatttt gcctctctgg cggtaattta ctttacgtaa aacatgcata
     5821 ctatgagcac atgtttaaat atgaacacat gtttaaaccg tctttgcagt atctatattc
     5881 ttgtggattg acagggttgg taatttttta ccagaggacg tgctatgcgc gaaaaggatt
     5941 atgtcgtaat tataggttcg gcgaatattg atgtcgccgg atattcacat gaatcattaa
     6001 attatgcgga ttcaaatcca ggtaaaataa aatttacgcc tggtggagta gggcgcaata
     6061 ttgcacaaaa cctggcgttg ctgggtaaca aagcctggct actgagcgcc gtaggcagtg
     6121 atttttatgg tcaatcgctg ctaacgcaaa ccaatcaatc tggtgtttat gtcgataaat
     6181 gcctgattgt gccgggagaa aatacgtcga gttatttatc attactcgat aataccggtg
     6241 aaatgctggt tgctataaat gacatgaata ttagcaacgc tattacagct gaatatctcg
     6301 cacagcaccg tgaatttatt cagagggcaa aggtcattgt cgcggactgt aatatcagtg
     6361 aagaggcact ggcatggatt ctggataatg ccgccaacgt acccgtattt gtcgatccgg
     6421 tttcagcgtg gaaatgtgtc aaagtgcgcg accgtctaaa tcagaaccac actctgaagc
     6481 caaaccgcct tgaagcggaa accctgagtg ggattgcgct gtcagggcgt gacgatgtgg
     6541 caaaagttgc tgcctggttc catcaacatg gcctgaaccg actggtattg agcatgggcg
     6601 gcgacggcgt ttattacagc gatatcagcg gtgaaaatgg ctggtctgcg ccgatcaaaa
     6661 ccaatgttat taatgttacc ggagcgggcg atgccatgat ggcgggactt gcttcgtgtt
     6721 gggtagacgg aatgccgttt gccgaatctg ttcgtttcgc acagggatgt tcgtcaatgg
     6781 cgctctcctg tgaatacacc aataaccccg atttatcgat tgccaacgtt atatcgttag
     6841 tggagaacgc agaatgtctg aattaaaaat ttcccctgaa ttattacaaa tttctccgga
     6901 agtgcaggac gctttaaaaa acaaaaaacc ggttgtggcg ctggaatcga ccattatttc
     6961 tcacgggatg ccgttcccac aaaatgccca gaccgcaatt gaagtagaag aaactattcg
     7021 taaacagggc gcagtacctg ccactatcgc cattattggc ggcgtgatga aagtgggttt
     7081 aagcaaagaa aaaattgaat tactgggtcg tgaagggcat aacgtgacca aagttagtcg
     7141 tcgcgattta ccttttgttg ttgccgccgg aaaaaatggc gcaaccactg tggcttcaac
     7201 gatgattatt gcggcgcttg ccggaattaa agtatttgcc accgggggaa ttggtggtgt
     7261 gcatcgcggg gcggaacata ccttcgatat ttctgccgat ttgcaagaac tggcaaatac
     7321 taatgtcacc gttgtttgtg ccggggcgaa atctattctc gatttaggat taaccactga
     7381 gtatttagaa accttcggtg tgccgttaat tggctatcag actaaagcgc tgcctgcgtt
     7441 tttctgccgt accagctcgt ttgacgtcag cattcgtctc gacagcgcca gtgaaattgc
     7501 ccgtgcaatg gcggtgaaat ggcaaagcgg gctgaacggt ggcctcgtgg tagcgaaccc
     7561 gatcccggaa cagtttgcga tgccggagga atctatcaat gcagccatag atcaagccgt
     7621 cgccgaagcc gaagagcagg gcgttattgg taaagaaagt acaccgttcc tgctggcgcg
     7681 cgttgctgag ctgaccagcg gtgacagcct gaaatccaat atccagctgg tgttcaacaa
     7741 cgccattctg gcgagtgaaa ttgccaaaga ataccagcgt ctcgcgggtt aatttctgac
     7801 gggcagaaag ccgtcgcccg tactcatcaa gcataccaaa acctggcgct gacgccgggt
     7861 ttactggcat gaagggaaaa tcattatgga tataatgaga agtgttgtgg ggatggtggt
     7921 gttactggca atagcatttc tgttgtcagt gaataaaaag agcatcagtt tgcgcacggt
     7981 tggagccgca ctgctgctgc aaatcgctat tggtggcatc atgctctact tcccaccggg
     8041 aaaatgggca gtagaacagg cggcattagg cgttcataaa gtgatgtctt acagtgatgc
     8101 cggtagcgcc ttcatttttg gttcgctggt tgggccgaaa atggatgtcc tgtttgacgg
     8161 tgcgggtttt atcttcgcct ttcgcgtact tccggcgatt attttcgtta ctgcgctcat
     8221 cagtctgctg tactacattg gcgtgatggg gctgctgatt cgcatccttg gcagcatttt
     8281 ccagaaagcc ctcaacatca gcaaaatcga atcttttgtt gcggttacca ctattttcct
     8341 cgggcaaaat gagatcccgg cgatcgttaa accgtttatc gatcgcgtga atcgcaacga
     8401 gttgtttacc gcaatttgta gcgggatggc gtccattgct ggttcggtga tgattggtta
     8461 tgccggaatg ggcgtaccaa ttgactacct gttagcggca tcgctgatgg cgatccccgg
     8521 cggtattttg tttgcacgta ttcttagccc ggcaaccgag ccttcgcagg tcacatttga
     8581 aaatctgtcg ttcagcgaaa cgccgccaaa aagctttatc gaagcggcgg cgagcggtgc
     8641 gatgaccggg ctgaaaatcg ccgctggtgt agcgacggtg gtaatggcgt ttgtcgcaat
     8701 tattgcgctg atcaacggca ttatcggcgg aattggcggc tggtttggtt tcgccaatgc
     8761 ctctctggaa agtatttttg gctatgtgct ggcaccgctg gcgtggatca tgggtgtgga
     8821 ctggagtgat gccaaccttg ctggtagcct gattgggcag aaactggcga ttaacgaatt
     8881 cgtcgcttac ctgagtttct ccccatacct gcaaacgggc ggcacgctgg aagtgaaaac
     8941 cgtggcgatt atatcctttg cgctttgtgg ttttgctaac tttggttcta tcggtgttgt
     9001 cgttggcgca ttttcggcta tttcgccaaa acgtgctcca gaaatcgccc agcttggttt
     9061 acgggcgctg gcagcagcaa cgctttccaa cctgatgagt gcgactattg ccgggttctt
     9121 tattggtctg gcgtaatgct cgatttgtag accggataaa acgtattaag cgttgcatcc
     9181 ggcaatcgcg ctattgcaaa taatttttac tgcatcatcc cggagaattt attctccggg
     9241 tccatctcca gcgccagtcc tgacaactgc tttctgtttt taattagata tcctttcttt
     9301 taattaataa accgtcgtga atgaattgcg cgagaacata taaaagatgt cgataagaaa
     9361 cgcctaaata ctctgccgct tgcgtatgct tttcgtgata aagatcacct tcttgtgaga
     9421 gtaatataaa tgctgccagg cgattcacta acggaaatga ttgattctga gttaaagaaa
     9481 caatattacg ataattttta tgacttaagg tgacgcaaag ttttcgtaaa aatagggtgt
     9541 cgtttaataa cagcggacgg taatgtttca tagggagcgc aaggcaccaa cactcttcaa
     9601 tagcctgcac cgcacgcggt tcatggtctt tgtcgattaa ctcaatctcg ccaataaaac
     9661 agggagcggc aaagaaatcg atcagcgaca cgcgaccatt ggcaagcgtg gcgtaaagcc
     9721 tggcgcggcc tcgcgtcaga taaaacagcc atgacggctg ttgcccttcc tgcacaatgt
     9781 agtcacgcgc taaaaaatga aacagccgcg tatcggctga aacatccgtt gaaaagcaat
     9841 ccttaaacgc agattcactc atcaattgct gcttaaggtc attattgtgg atttctttca
     9901 cgatgtttat ccgctatgag atttctcata tttagcatac gtacgctgtt gttaagattc
     9961 aatcattggc ccggtcgaat tttctttgct gtagaaaaaa ataacgcaac tggaaacaga
    10021 ggaaataaac aatggaaaag agaaaaatta ttctggattg tgagccgggt catgatgatg
    10081 ctattgcaat gatgatggca gcgaaacatc cggcaataga tttattaggc atcactattg
    10141 tagcgggtaa tcagacgctt gataaaacat taattaatgg cctgaatgtt tgccagaaac
    10201 tggagattaa tgttccggtt tatgcgggga tgccgcaacc cattatgcgt aaacaaatcg
    10261 ttgccgataa tattcacggt gaaaccggac tggatggccc ggtattcgag ccgctgaccc
    10321 gccaggcaga aagtacccat gcggtgaaat atatcatcga taccctgatg gcaagcgatg
    10381 gcgatatcac tctggtgccg gttggtccgc tttcaaatat cgcggtggca atgcgtatgc
    10441 aacccgcaat cctgcccaaa atccgtgaaa ttgtgctaat gggcggcgct tacggtacag
    10501 gcaacttcac cccatctgcc gagttcaata tctttgccga cccggaagcc gcccgcgtgg
    10561 tgttcacctc cggcgttcca ttagtgatga tgggcctcga tcttaccaac cagaccgttt
    10621 gcaccccgga tgtaattgct cggatggaaa gggtaggcgg ccctgccgga gagctgttca
    10681 gcgacatcat gaacttcact ctcaaaacgc agttcgaata ctacggcctg gctggcggcc
    10741 cggtgcatga cgccacctgc attggttatc tgatcaaccc tgatggcatt aaaacccagg
    10801 atatgtacgt cgaagtggac gtcaacagcg gcccttgcta cgggcgtacg gtctgcgacg
    10861 aactgggcgt tcttggcaag cccgctaata ccaaagtcgg catcactatt gataccgact
    10921 ggttctgggg cttagtcgaa gagtgcgtgc gcggctacat caaaacccat taagtgttga
    10981 taacaagccg ggacagtatc gcaagatgca ggcccggcta tttctgatgg gcagaaagcc
    11041 gtcgcccaaa atgacatgaa gagataatca ctatggatgt catgagaagt gttctgggaa
    11101 tggtggtact gctggcaatt gcgtttttac tgtcagtaaa caagaagaag atcagcctgc
    11161 gtaccgttgg cgcggcgtta gtgttacagg tcgtgattgg cgggattatg ctttggttac
    11221 cgccagggcg ttgggtcgca gaaaaagtcg cttttggcgt ccacaaagtg atggcgtaca
    11281 gcgacgcagg tagcgcattt atcttcggtt ctctggtcgg accgaaaatg gataccttat
    11341 ttgatggcgc aggatttatc tttggtttca gggtattacc ggcaattatc ttcgtcactg
    11401 cactggtgag tattctctac tacatcggtg tgatggggat cttaattcgc attctcggcg
    11461 gtatcttcca gaaagcttta aatatcagca agatcgagtc attcgtcgcg gtcaccacca
    11521 ttttcctcgg gcaaaacgaa attccggcga tcgtcaaacc ctttatcgat cgtctgaatc
    11581 gcaatgaatt atttacagcg atttgtagtg gcatggcctc gattgctggt tcgacaatga
    11641 ttggttacgc cgcactgggc gtacctgtgg aatatttgct ggcggcatcg ttaatggcga
    11701 tccctggcgg gatcttgttt gcccgcctgt taagcccggc tacggaatct tcgcaggttt
    11761 cttttaataa cctctctttc accgaaacac cgccaaaaag cattattgaa gccgatgcga
    11821 caggggcaat gaccgggctg aaaatcgccg ccggtgtagc gacagttgtt atggcatttg
    11881 tcgccatcat tgcgttaatt aacggtatta tcggcggcgt tggcggctgg tttggttttg
    11941 aacatgcctc gctggagtcc attttaggtt acctgctggc cccacttgcg tgggtaatgg
    12001 gtgttgactg gagtgatgca aatcttgccg ggagtttgat tggacagaaa ctggcaataa
    12061 atgaatttgt cgcttatctc aatttctcac cctatctgca aacggctggc actctggatg
    12121 ctaaaaccgt ggcgattatt tccttcgcgt tgtgcggttt cgctaacttt ggttctatcg
    12181 gggtggtggt gggggcgttt tctgcggttg cgccacaccg tgcgccggaa atcgcccagc
    12241 ttggtttacg cgcgctggcg gcggcgacac tttctaatct gatgagtgcc accattgccg
    12301 ggttctttat tggtttagcg tagtgtgcaa tttatgagcc ggattagatg cggtcaagcg
    12361 tcgaatccgg caacattgtt acaccattgg cactaatgac agcgcgttat cggcagacag
    12421 ggtagggttg tttaagctgc cgctggcgcg tgaaattgcc gcgcaggcca tcgcaaaacg
    12481 ggcgctgtca cggaaattgt ttccctccag aaagctatat accagacccg ccataaaacc
    12541 atcgtcagca ccaaaactgt ctaccgtcgt atgcgctggc acagtcagca aaaattgttc
    12601 accatccttt tcgctgcaat aaactgactc atcgggcaaa taaacaaaca gttgctgaac
    12661 accttgctga tgcaatgcat tcactgcggc attacggtca gcatcgctgg tgatcgcctg
    12721 tccccataaa atttccagct ccggtaaagt gggtttcagg gtgtgaatat gcgccagcca
    12781 gtgtttgatt ttgcccgctt tgaattctga aacggtatcg acaaacaccg ggatctcatc
    12841 agcgagggta aagacccatt ccagcgcctc ggctgtcagg ttgcaatcag ccagtaccac
    12901 gcccgcatga cgaagtaaat cgcgcgaccc gttcagtagt tgcggtgtca actgttccag
    12961 cagatgggta tcgttaattg ccagcacggt ttgatcgtct cgattggcaa ttgccagata
    13021 cgtcgatgtg ctttgaccat gcaaacgaac gcagccggag acattcacgc cggcacggcg
    13081 cgtttcttcc aggagcattt cgccataaaa gtcatcgcca atcactgaaa gcaaatggac
    13141 gtcacggcct aacagcgcca gattgtgggc gatattgcgt ccaacgccgc ctgccgaaca
    13201 atgaattgta ccgggatgag aagccgcttg cgggtaatgg atatccgcca tcccgcgaat
    13261 atccatattg attgtcccca ccactacgca gtattcctgc tcggtgagaa tgtaaccttt
    13321 gtctttaatc cggcctttgc gcattaaatc cataatatgc gccgcaacgc gcgaacgact
    13381 gatttgcagc atgtccgcaa tttcgttctg ctgaatcagc gggttacgcc gtaaaattgc
    13441 aaggatctcc ttttcccggt tattcatctt caggctaccg ctttttcagt ttgttgcgct
    13501 ttcagccagg cgatctcttc tgcccagata tccgggttga tggtttcaag gatcagcgga
    13561 atgccgtcga aacggtcgtt ctgcataatc cagcggaacg catcatgacc gatattgcct
    13621 tcaccgaggc tatgatggcg gtcaacgcgg ctgccaaagg tacttttcgc atcgttaagg
    13681 tgcatcccgc gcaaatactt aaagccgaca atacgggcaa aatcagcgaa cgttttttcg
    13741 cattcggctg gagtacgcaa atcatacccg gcagcgaaag catggcaggt atcaatgcag
    13801 acgccgacgc gggatttatc ttccacgccg tcgataatcg ccgcgagatg ttcgaattta
    13861 aaccctaagt tactgccctg accggcggtg ttttcaatca ccgctgttac accttgagtt
    13921 ttatccagcg caatgttgat ggattcggca atacgcgcaa ggcaatcctc ttctgaaatc
    13981 tgcatcagat ggctgccagg gtggaagttg agcaaagaaa gccccagttg ttcgcaacgc
    14041 tgcatttcat ctataaaggc atcgcgcgat ttttccagag cttcagtgac cggatgtccg
    14101 aggttgatca gataactgtc gtggggaaga atttgcgccg atgtgtagtg atatttttca
    14161 caggcggctt tgaattcatc gatggtttgc gtcgtgagcg gtgcggcacg ccactgacgt
    14221 tggtttttgg tgaacaaggc aaacgcggtt gcgtcgattt cggcggcgcg aattgcggca
    14281 tttgccagac cgccagcagc actaacgtgc gctccaatgt atttcatgcg aggactcctg
    14341 ttaaacccgc tggaggaaaa cggtaatgat agcgggttaa caggagcaag aagtagtggt
    14401 ttatgcgatg acgctttgaa tcacatagtt aatcgcacca ccaccaacaa tcagccaggc
    14461 aaacagtacc agtgccatca gcagaggttt cgccccagct tttttcagcg cgctgacgtg
    14521 agtggtcaga cccagcgccg ccatcgccat tgccagcagg aaggtatcca gcgttaccag
    14581 catgttcacc acgctctgcg gtaacaggtg gaacgagtta aagatggcaa ctacgatgaa
    14641 caagatggca aaccacggaa tagtgatttt gcttttctcg ccgctgttcg ccccagacag
    14701 ctgtttaaca cgcgccgcca gcaggatgag gaacggagcc agcatcatca cgcgcagcat
    14761 tttggaaata actgctgcgt tttctgcatc cgggctgatg gcatgacctg ccgccaccac
    14821 ctgcgccact tcgtgcacag tagaaccaat gtagataccg aaagtttccg gactaaacca
    14881 ttgagacatc agcggatata tcgccgggta gaggaaaatc gcgacggtcc cgaagataac
    14941 aacggttgca acagccacgg ttactttact ggcttccgct ttcactaccg gctcagtcgc
    15001 cagtaccgcg gcagcaccac agatactgct accggcaccg atcaaccaac tggtgtgctt
    15061 atccagacca aacactttct gccccaggaa gcaagccagc aggaaggtac tggacagcgt
    15121 cagcacgtca atgatgatcc cactgatacc gacatcggca atttgcgaga acgtcagacg
    15181 gaagccataa agaatgatac ccagacgtaa taaatattgc ttggcaaaca gcacaccacc
    15241 gtcacagctt ttccagatgt gcggatagat ggtgttgcct aaaaccatcc ccaacaagat
    15301 tgcgagggtg agggcactaa acccggcacc cgcaaccgcg ggaatggaac caccccacag
    15361 ggcgaccccg gtgataactg cactcagggc taaccccgga ataaaatgcc acagtgtacg
    15421 atgttgtttc tgtaaggtga tattcgtcat aaccctctcc tttaaccggg cataaggtta
    15481 cgactaactg gtttaaaaat aaaattgatt atatatttat aattaatctt tataagtggt
    15541 aagcgactat gcacatcacc ctccggcagt tggaagtttt tgcagaagta ttgaaaagtg
    15601 gatcaaccac ccaggcgtcg gtgatgctgg cgttgtcgca atcagcagtg agcgcagcct
    15661 tgactgacct ggaagggcag cttggcgttc aactgtttga tcgcgtgggg aaaagactgg
    15721 ttgttaatga acacgggcgg ctgctctatc cgcgagcgtt ggcactgctt gaacaggcgg
    15781 ttgaaatcga acaactgttt cgcgaagaca acggcgcgat tcgtatctat gccagtagta
    15841 ccatcggtaa ctacattctg cctgcagtta tcgcccgtta tcgccatgat tatccgcagt
    15901 tgccgattga acttagcgtt gggaacagcc aggacgtgat gcaagcggtg ctggatttcc
    15961 gcgttgatat tggctttatt gaaggaccgt gccacagcac tgaaatcatt tctgaaccgt
    16021 ggctggaaga cgagctggtg gttttcgccg cgccgacttc gccgttggct cgtggtccgg
    16081 tcaccttaga acagctggcc gctgcgccgt ggatcctgcg tgaacgcggt tccggcacgc
    16141 gggatattgt cgattatctg ttgctgtcac atttaccgaa gtttgagatg gcgatggaat
    16201 taggtaactc cgaggcaatc aaacatgcgg tgcgtcatgg gttgggaatt agttgcctgt
    16261 cgcgacgtgt gattgaagat caattgcagg caggcacatt aagtgaagtt gcggtccctc
    16321 tgccgcgcct gatgcgtacg ttgtggcgta tacatcatcg gcaaaaacac ctttccaacg
    16381 cgctacggcg ctttctggac tattgcgatc ccgcaaatgt gccgcgttaa gttgctgtac
    16441 aagaacatgc tggtgctgtg tcgatttcgt gacgcagcgc cttcagcatg cattcgccag
    16501 aaaagagatt ggctgcttta cttataatcc ctgggcgatc atgaaggtgt cttataaccg
    16561 tgtatttctg ccggaaggat tgccaatcgt ctgctacaat cgcgccatca tttttaagat
    16621 ggatagcatt tttgtatggg ttccgaaact aaaactacag aagcgccggg cttacgccgt
    16681 gaattaaagg cgcgtcacct gacgatgatt gccattggcg gttccatcgg tacaggtctt
    16741 tttgttgcct ctggcgcaac gatttctcag gcaggtccgg gcggggcatt gctctcgtat
    16801 atgctgattg gcctgatggt ttacttcctg atgaccagtc tcggtgaatt ggctgcatat
    16861 atgccggttt ccggttcgtt tgccacttac ggtcagaact atgttgaaga aggctttggc
    16921 ttcgcgctgg gctggaacta ctggtacaac tgggcggtga ctattgccgt tgacctggtt
    16981 gcagcacagc tggtcatgag ctggtggttc ccggatacac cgggctggat ctggagtgcg
    17041 ttgttcctcg gcgttatctt cctgttgaac tacatctctg ttcgcggctt tggtgaagcg
    17101 gaatactggt tctcactgat caaagtcacg acagttattg tctttatcat cgttggtgtg
    17161 ctgatgatta tcggtatctt caaaggcgcg cagcctgcgg gctggagcaa ctggacaatc
    17221 ggcgaagcgc cgtttgctgg tggttttgcg gcgatgatcg gcgtagctat gattgtcggc
    17281 ttctctttcc agggaaccga gctgatcggt attgctgcag gcgagtccga agatccggcg
    17341 aaaaacattc cacgcgcggt acgtcaggta ttctggcgaa tcctgttgtt ctatgtgttc
    17401 gcgatcctga ttatcagcct gattattccg tataccgatc cgagcctgct gcgtaacgat
    17461 gttaaagaca tcagcgtcag tccgttcacc ctggtgttcc agcacgcggg tctgctctct
    17521 gcggcggcgg tgatgaacgc agttattctg acggcggtgc tgtcagcagg taactccggt
    17581 atgtatgcgt ctactcgtat gctgtacacc ctggcgtgtg acggtaaagc gccgcgcatt
    17641 ttcgctaaat tgtcgcgtgg tggcgtgccg cgtaatgcgc tgtatgcgac gacggtgatt
    17701 gccggtttgt gcttcctgac ctccatgttt ggcaaccaga cggtatacct gtggctgctg
    17761 aacacctccg ggatgacggg ttttatcgcc tggctgggga ttgccattag ccactatcgc
    17821 ttccgtcgcg gttacgtatt gcagggacac gacattaacg atctgccgta ccgttcaggt
    17881 ttcttcccac tggggccgat cttcgcattc attctgtgtc tgattatcac tttgggccag
    17941 aactacgaag cgttcctgaa agataccatt gactggggcg gcgtagcggc aacgtatatt
    18001 ggtatcccgc tgttcctgat tatttggttc ggctacaagc tgattaaagg aactcacttc
    18061 gtacgctaca gcgaaatgaa gttcccgcag aacgataaga aataagtttc ctcccttcct
    18121 tgctaagccc tctcaaccga gagggctttt tcaattccat ttccctgaca aatcatgcgg
    18181 atataaaatt taacatttgg attgataatt gttatcgttt gcattatcgt tacgacgcaa
    18241 tcaaaaaagg ctgacaaatc agaggctgtt ccggctttct gggatgatca cctgcataca
    18301 aataagtcca ccgcgatgct gccgtacgca aggggacgta aagaagatgt gagcgatacc
    18361 cattttattt tcgtagttac ctcatggaga tatggaatgt ttaggttgaa ccctttcgta
    18421 cgggtcgggc tgtgtttgtc cgctatttct tgtgcatggc ctgtgttagc ggtcgatgat
    18481 gatggcgaaa cgatggttgt cactgcatct tccgtggaac aaaatcttaa agatgcaccc
    18541 gccagtatca gcgtcattac ccaggaagac ctgcagcgaa aaccggtaca gaatctgaag
    18601 gatgtcctca aagaagtgcc tggcgtacaa ctgacgaacg aaggggataa ccgtaagggc
    18661 gttagtattc gtggtctgga cagcagctac accctgattc ttgtcgacgg taaacgcgtt
    18721 aactcccgca atgccgtctt ccgccacaat gatttcgatc tgaactggat cccggtcgat
    18781 tccatcgaac gtattgaagt ggttcgtggc ccgatgtcgt cgctatacgg ctccgatgcg
    18841 ctcggcggtg tagtgaatat catcaccaaa aaaatcggtc agaaatggtc gggtaccgtt
    18901 accgtcgata ccaccattca ggaacatcgc gatcgcggtg atacctataa cggtcagttc
    18961 tttaccagtg gaccattaat tgatggtgtg ctgggaatga aagcttacgg cagcctggca
    19021 aaacgtgaaa aggatgaccc gcaaaactca acgaccaccg ataccggaga aacgccgcgt
    19081 attgaaggat tctccagccg cgacggcaat gtcgaatttg cctagacacc gaatcaaaat
    19141 cacgatttta ctgccggata cggtttcgac cgtcaggatc gtgattccga ttcgctggac
    19201 aaaaaccgcc tggaacgcca gaactactgc gtcagccata atgggcgttg ggattacggc
    19261 accagcgaac tgaaatccta cggtgagaaa gtcgagaaca aaaaccctgg caacagcagc
    19321 ccgataactt ccgaaagcaa tacggtcgac ggcaaataca cgttgccgct gacggcgatt
    19381 aatcagtttc tcacggttgg cggtgaatgg cgtcacgaca aacttagcga tgcggtgaac
    19441 ctgaccgggg gaaccagctc caaaacgtct gccagccagt acgcgctgtt tgtggaagat
    19501 gaatggcgga tcttcgagcc gctggcgctg acgaccggcg tgcgtatgga cgatcacgaa
    19561 acctacggtg aacactggag tccgcgtgcc tacatggttt ataacgccac cgacaccgta
    19621 acggtgaaag ggggctgggc gacggcattt aaagcacctt ctctgttgca acttagccct
    19681 gactggacga gcaattcctg ccgtggcgca tgtaagattg tgggtagccc ggatctgaaa
    19741 ccagaaacca gcgaaagttg ggagctgggg ctttactaca tgggtgaaga aggctggctg
    19801 gaagggggtg aatccagcgt tatcgttttc cgtaacgatg tgaaagatcg tatcagcatc
    19861 agccgtacgt ctgacgtcaa cgctgcaccg ggctaccaaa actttgttgg ttttgagacg
    19921 ggcgctaacg gacggcgcat accggtattt agctactaca gcgttaacaa agctcgtatt
    19981 cagggcgtgg aaaccgaact gaaaattccg ttcaacgatg aatggaaact gtcgatcaac
    20041 tacacctaca acgatggtcg tgatgtcagc aacggcgaaa acaaaccgct atccgatctg
    20101 ccgttccata ctgctaacgg tacgctggac tggaaaccgc tggcgctgga agactggtca
    20161 ttctatgttt ctggtcacta taccgggcag aaacgcgccg acagcgcgac ggctaaaaca
    20221 ccgggcggtt ataccatctg gaataccggc gcggcctggc aggtgactaa agacgtcaaa
    20281 ctgcgcgcag gcgtgctgaa ccttggcgac aaggatctca gtcgtgacga ctacagctat
    20341 aacgaagacg gacgtcgtta ctttatggca gtggattatc gcttctgatg agaagatgcc
    20401 cggcgaaccg ggcggacttt cacttcagta aatactgcgc atggaagcgc aggtgatcct
    20461 ctataaaaga ggcgatgaag tagtaactgt gatcatatcc cggctgaata cgcagcgtca
    20521 tcggccacgc tttctggcgc gcggcttctg ccaataccgc aggttgcaac tggtcggcaa
    20581 gaaactgatc attatcgccc tgatcgataa gcgtcgggat cgcatcctgc gcgttactgg
    20641 catacatcag cgcgcaacta tcccattcca gcatgcattt ttgtcctcac ctaaataggt
    20701 gctaaacgct ttgattcccc acgggacgct gcacggattc acaatgggcg caaaggccga
    20761 aacgctggtg tatttacccg gatttttcag cgccataatc agcgcaccgt gaccacccat
    20821 tgagtgaccg ctaatggcgc agcggtcgct gacattaaat tgcgactgaa ccagcgccgg
    20881 taattcatcg cgcagataat catacatccg gtaatgcgtc gcccacggcg gttgcgtggc
    20941 attaagataa aagcctgcgc cctggcctaa atcgtagcca tcgtcgttgg caaccttttc
    21001 gccgcgcggg ctggtgtctg gcatcaccag tacaatcccc agttccgccg ctacccgctg
    21061 ggcacccgcc ttggtggtga agttctcgtc attgcaggtt aatccggaaa gccagtacag
    21121 cactggtggc ggggtgtgat cacgaggggg ggggagaaag atactgaacg tcatcgggca
    21181 gtttaaggta ctggagtcgt gtcgccagcg ttgctgccag ccttcaaaac agcggtgctc
    21241 ttcgagcatt tccatgcgtg gctccttgtt gtgttgtttg caatttcttc atcataccgt
    21301 ttattcaatg tgctgtgagt aactttcact tccgtatttg cataacgatg ttttaacatc
    21361 tgctgatgaa aggcagcggc aattacaata attatcgctg tgaatactgg attatgtgcg
    21421 ccgcctcacg cacaataatc aagctgtaaa tcagcttaat aactttgccc ccacgcaggg
    21481 tggaggcgtc acacctgcag gagaaatcat aaatgccatc actcagtaaa gaagcggccc
    21541 tggttcatga agcgttagtt gcgagaggac tggaaacacc gctgcgcccg cccgtgcatg
    21601 aaatggataa cgaaacgcgc aaaagcctta ttgctggtca tatgaccgaa atcatgcagc
    21661 tgctgaatct cgacctggct gatgacagtt tgatggaaac gccgcatcgc atcgctaaaa
    21721 tgtatgtcga tgaaattttc tccggtctgg attacgccaa tttcccgaaa atcaccctca
    21781 ttgaaaacaa aatgaaggtc gatgaaatgg tcaccgtgcg cgatatcact ctgaccagta
    21841 cctgtgaaca ccattttgtt accatcgatg gcaaagcgac ggtggcttat atcccgaaag
    21901 attcggtgat cggtctgtca aaaattaacc gtatcgtgca gttctttgcc cagcgtccgc
    21961 aggtgcagga acgtctgacg cagcaaattc ttattgcgct acaaacgctg ctgggcacca
    22021 ataacgtggc tgtctcgatc gacgcggtgc attactgcgt gaaggcgcgt ggcatccgcg
    22081 atgcaaccag tgccacgaca acgacctctc ttggtggatt gttcaaatcc agtcagaata
    22141 cgcgccacga gtttctgcgc gctgtgcgcc atcacaactg attaaaaggc aggaaccatg
    22201 gagcgcaacg tcacgctcga ttttgttcgc ggcgtcgcca ttctggggat cctgctatta
    22261 aacatcagcg cctttgggct accaaaggcg gcttatctca atcccgcctg gtacgatgct
    22321 attacgccgc aggatgcatg gacctgggca tttctcgatc tcatcggcca ggtgaaattc
    22381 ctcacgcttt ttgcgctgct gtttggtgct ggcctgcaaa tgttgctgcc ccgtggcaga
    22441 cgctggatcc agtcgcggtt aacgctgtta gtcttgttgg gctttattca cggtttattg
    22501 ttctgggacg gcgatattct gctggcttac gggctggtgg ggcttggcgt tttgctgtta
    22561 ttggggctga tttccgacag ccagaccagc cgcgcctgga cgccggatgc atcggctatt
    22621 ttgtatgaaa aatactggaa gcttcacggc ggcgttgaag cgatcagtaa tcgtgccgat
    22681 ggtgttggca acagtttact ggcactgggc gcacagtatg gctggcaact ggcagggatg
    22741 atgctcattg gtgcggcgct gatgcgcagc ggctggctga aagggcagtt cagcttacgt
    22801 cactatcgtc gtactggttt tgtgctggtg gcgattgggg tgaccattaa ccttcctgcc
    22861 atcgccctgc aatggcagct ggactgggcg tatcgctggt gtgccttctt acttcaaatg
    22921 ccgcgggaac tgagtgcgcc gtttcaggcg attggctatg cgtcgctgtt ttatggcttc
    22981 tggccgcaat tgagccgctt taagctggtg cttgcgatcg cctgcgtcgg acggatggcg
    23041 ctgaccaact atctattgca aacgctgatt tgtaccacgc ttttttacca cctcggtttg
    23101 tttatgcatt ttgaccgcct ggagctgctg gcgtttgtta ttccggtatg gctggcgaat
    23161 atcctcttct ctgttatctg gctgcgttac ttccgccagg ggccggtgga atggctctgg
    23221 cgtcagttaa ctttgcgtgc tgccggaccg gcaatatcta aaacatcaag ataacgatct
    23281 ggattacatt cgttaacaaa acggctgtaa ccgtttccat tgctgtgact cgattcacga
    23341 agtcctgtat tcagtgctga caaaatagcc gccagcaagc agtcatttac tgcaatctca
    23401 taacaggtag tgaatatgat caccattcgt gatgtagcgc gtcaggctgg cgtctctgtg
    23461 gcaacggttt cccgggtgct caataacagc acgctggtca gtgccgacac gcgtgaagca
    23521 gtaatgaaag ccgtgagtga gctggattat cggccaaacg ccaatgccca ggcgctggca
    23581 actcaggtta gcgacaccat tggcgtggtg gtgatggacg tttctgatgc gtttttcggc
    23641 gcgctggtaa aagcggtgga tctggtcgcc cagcagcatc agaaatacgt gctaatcggc
    23701 aatagctatc atgaagcgga aaaagagcgt cacgccattg aggtgttaat tcgccagcgt
    23761 tgtaatgcgt tgattgttca ctcaaaagca ttgagtgacg atgaactggc gcaatttatg
    23821 gataacattc ccggtatggt gttaatcaac cgcgttgtgc cggggtacgc ccatcgttgc
    23881 gtttgcctgg ataatctcag cggtgcccga atggcgacgc gcatgttgct gaataacggt
    23941 catcaacgta ttggttatct ttcttccagc cacggcattg aagatgacgc catgcgtaaa
    24001 gcaggctgga tgagtgcgtt gaaagagcag gatattattc cgccggaaag ctggattggc
    24061 actggtacgc cggacatgcc gggcggtgag gcggcgatgg ttgaactgct ggggcgcaat
    24121 ctacaactta ccgctgtatt tgcttataac gacaatatgg ccgctggcgc actgacagca
    24181 ttaaaagata atggcattgc gattccgtta catctctcaa tcatcggttt cgatgatatt
    24241 cccatcgccc gttacaccga cccgcaatta acgaccgtgc gttatcccat tgcttcaatg
    24301 gctaaattag ccaccgaact ggccttgcag ggggcagcag gcaatattga tcctcgtgcc
    24361 agccactgtt ttatgccgac gttagtgcgt cgccattctg tcgcaacgcg ccagaatgcg
    24421 gcggcgatca ctaactcaac aaatcaggcg atgtaaccgc tttcaatctg tgagtgattt
    24481 cacagtatct taacaatgtg atagctatga ttgcaccgtt ttaatgttgt aacccgtatg
    24541 taacagtgaa taatcacttt caccgaggta atagcgtcat aacaacaatt aaagccgttt
    24601 tctggagcgt taccgggcat ggaagaacga attttaaaaa gtgagcttcg gcgttcagta
    24661 acacttcatt aactctactg ccccgccgag catttatctc aagcactacc ctgcataaga
    24721 aaaaccggag ataccatgaa taagaaggtg ttaaccctgt ctgctgtgat ggccagcatg
    24781 ttattcggtg ccgctgcaca cgctgctgat actcgcattg gtgtaacaat ctataagtac
    24841 gacgataact ttatgtctgt agtgcgcaag gctattgagc aagatgcgaa agccgcgcca
    24901 gatgttcagc tgctgatgaa tgattctcag aatgaccagt ccaagcagaa cgatcagatc
    24961 gacgtattgc tggcgaaagg ggtgaaggca ctggcaatca acctggttga cccggcagct
    25021 gcgggtacgg tgattgagaa agcgcgtggg caaaacgtgc cggtggtttt cttcaacaaa
    25081 gaaccgtctc gtaaggcgct ggatagctac gacaaagcct actacgttgg cactgactcc
    25141 aaagagtccg gcattattca gggcgatttg attgctaaac actgggcggc gaatcagggt
    25201 tgggatctga acaaagacgg tcagattcag ttcgtactgc tgaaaggtga accgggccat
    25261 ccggatgcag aagcacgtac cacttacgtg attaaagaat tgaacgataa aggcatcaaa
    25321 actgaacagt tacagttaga taccgcaatg tgggacaccg ctcaggcgaa agataagatg
    25381 gacgcctggc tgtctggccc gaacgccaac aaaatcgaag tggttatcgc caacaacgat
    25441 gcgatggcaa tgggcgcggt agaagcactg aaagcacaca acaagtccag cattccggtg
    25501 tttggcgtcg atgcgctgcc agaagcgctg gcgctggtga aatccggtgc actggcgggc
    25561 acagtactga acgatgctaa caaccaggcg aaagcgacct ttgatctggc gaaaaacctg
    25621 gccgatggca aaggtgcggc tgatggcacc aactggaaaa tcgacaacaa agtggtccgc
    25681 gtaccttatg ttggcgtaga taaagacaac ttggctgaat tcagcaagaa ataagactga
    25741 ttgggcgcag tcgtttactg cgcccttatt gtatggccaa caaggtaaaa ttatatggtc
    25801 agctcaacga ctccgtcttc cggggaatac ttgttggaaa tgagcggtat caacaagtcc
    25861 tttcctggtg ttaaggcact tgataacgtt aatttaaaag tccggccaca ttctatccat
    25921 gcattaatgg gggaaaacgg tgcaggaaaa tcgacattat taaaatgcct gtttggtatt
    25981 tatcaaaaag actccggcac cattttattc cagggtaaag agatcgattt ccattccgcc
    26041 aaagaggcac tggaaaatgg tatttcgatg gtacaccagg agttaaacct ggtattacaa
    26101 cgttcggtga tggataacat gtggctgggg cgatatccca ccaaaggcat gtttgtcgat
    26161 caggacaaaa tgtaccgcga aaccaaagcg atttttgatg aactggatat tgatatcgat
    26221 ccgcgtgcgc gcgtcggcac attatccgtt tcgcaaatgc agatgatcga aatcgccaaa
    26281 gcgttttcct ataacgcgaa aattgtgatt atggatgaac cgacttcttc gttaaccgaa
    26341 aaagaggtca atcatctgtt cactattatt cgtaaattaa aagagcgcgg ctgcggtatt
    26401 gtttatatct cgcataaaat ggaagaaatc ttccagttat gtgatgaagt taccgtattg
    26461 cgcgacggtc agtggatcgc caccgaaccg ctggcaggac tgacgatgga caagatcatc
    26521 gccatgatgg ttgggcgttc tcttaatcag cgttttcctg acaaagaaaa caagccgggc
    26581 gaagtcatcc tcgaggtgcg taacctaacg tcactgcgcc agccgtcgat tcgcgatgtc
    26641 tcgtttgatc tgcataaagg ggagatcctc ggtattgccg ggctggtggg ggcgaaacgt
    26701 accgatattg ttgagacgtt atttggtatt cgcgagaaat cggctggcac cattacgttg
    26761 cacggcaaac agatcaataa ccataatgcc aacgaagcca taaaccacgg atttgcactg
    26821 gtaactgagg agcgccgctc aacgggaatt tatgcctatc tggatattgg ttttaactcg
    26881 ttaatttcca atattcgcaa ctacaaaaat aaagttggtt tactggataa ctcgcggatg
    26941 aaaagcgata cccagtgggt gattgattcg atgcgggtaa aaacgccggg tcatcggacg
    27001 caaattggtt cgctctccgg tggtaatcag caaaaggtga ttattggtcg ctggctacta
    27061 acgcaaccag aaatattaat gctcgatgaa ccgacgcgcg gtattgatgt cggggcgaag
    27121 tttgaaattt atcagttaat tgccgaactg gcgaagaaag gcaaggggat tattattatc
    27181 tcctctgaaa tgcctgagtt gttagggata acagaccgta ttctggtcat gagcaatggt
    27241 ctcgtttccg gaattgtcga tacaaaaaca acaacgcaaa acgaaattct gcgtcttgcg
    27301 tctttgcacc tttaagatta ggggcttccc atgagtgcgt taaataagaa aagttttctt
    27361 acttacctga aagagggcgg tatttacgtc gttcttttag ttttgctggc gattattatt
    27421 ttccaggacc caacattttt aagtctgttg aacttaagta atattctcac ccagtcatcg
    27481 gtgcgtatta ttatcgcgct cggtgtggca gggttaattg tcacccaggg gaccgatctt
    27541 tctgctggtc gtcaggtagg gctggcggca gtggtggctg cgacattatt gcagtccatg
    27601 gataacgcca acaaagtgtt cccggaaatg gcgacgatgc cgattgcgct ggttattctg
    27661 attgtctgtg ccattggtgc ggtgatcggt ttgatcaacg gtctgattat cgcttatctc
    27721 aacgtgacgc cgttcattac cacgctcggc acgatgatca tcgtctatgg catcaactcg
    27781 ctctattacg actttgtcgg ggcgtcgcca atttctggtt ttgacagtgg cttctctacc
    27841 tttgctcagg gctttgtcgc gctggggagt ttccgtctct cttacatcac cttctacgcg
    27901 ttgattgcgg tggcgttcgt ctgggtgttg tggaacaaaa cccgcttcgg taagaacatt
    27961 tttgccattg gcggtaaccc ggaagcggca aaaatatctg gtgtcaacgt cggcctgaac
    28021 ctgctgatga tctacgcgtt gtctggcgtg ttctatgcct ttggcgggat gttagaagcc
    28081 ggacgtatcg gctctgccac caacaacctc ggctttatgt atgagctgga tgctatcgcg
    28141 gcgtgcgtgg taggcggtgt atcgttcagc ggcggtgtgg ggacggtgat tggcgtggtg
    28201 accggggtaa ttatttttac cgtcatcaac tatggcctga cgtatatcgg cgtaaaccca
    28261 tactggcagt acatcatcaa aggggcgatt attatcttcg ccgtagcgct ggattcactg
    28321 aaatacgcgc gtaagaagtg atgtgattgc ggatattgcc ggatgcgtct ctggttttac
    28381 aacgttaccg ggtgttcttt ctcgcctttc ttaaacttca cttccccgag ctcaatacaa
    28441 cccaccgggc agacgtgacc acaaagcaga caacccacac atttctcggt attacaatgc
    28501 ggtgtgcggg ttttctcgct ccattccatc gcctggtgac cgccgtcgta acaggaaata
    28561 taacagcgtc cacagccaac acatttgtca agattgatac ggggatagac aatataactg
    28621 cggtctaaat cttccgccgg gacaatattg ttattcgcca gacctaccat ctcctgcaac
    28681 gaatcaaatc cctgatcggc gagataatgc gataacccgc tcgccatatc ttctacgatg
    28741 cgatagccgt actgcataat gccggtggtg acctgtaacg ttgctgcgcc gagcaataaa
    28801 aactccgccg catcttccca ggtttcaatg ccgccgatac cgctgattgg gaaatcgcgc
    28861 agttctggat gggtgcgcat ttgctgaatg aagcgcaggg cgatcggttt taccgcttta
    28921 ccggaatatc cggaaatact cgatttaccg ttaacgatcg gcataccgat tttctgatta
    28981 agatcgatat tggtgatgga tttaacggtg ttaattgccg caatgccatc tgcgccgccg
    29041 cgcttcgccg ccagcgccac ttcgcacata tcaccgatat tcggcgtcat cttcgccagc
    29101 attggcagcg tggaaccccg tttcactgcc cgacaatatt tttctaccag ctccgggctt
    29161 tgcccgacat cgctacccat cgcatgagaa gtcatttgcg gacaggagaa gttacactcg
    29221 atcatatccg cgccagctgc tcccattgct gctcattttc ccccatgatc gaagcgatca
    29281 atactttgtc cgggtaatct tccttcagcc gacgcagggc ggtcagattc tcttccaacg
    29341 gatgttcagc aatctgctcc atatttttga agccgataaa accggtatct tctttcacca
    29401 gatgatcaaa acgcggcgag acttcgttgg cgataaaaaa gccgatcgtt ttaaacacca
    29461 caccgcccca acctgtgtcg taggctttgg cacacatctc atagcagttg cctaccggcg
    29521 aagaagagag gcagaacggg ttgggaaagt tcacgccgca aaaagtaatc gaaagatctt
    29581 tcgttaacat gagcaagctc cctctaaata gtgatgaatc gcctcggcgg cttctttccc
    29641 ggttttcacg gcatagacca cggttttgtc accctcaaca atatcgccag cagcaaagac
    29701 ttgcggatcg cggatctgat aattttgtgt tttgatggtg ttacgctgct gctctaactc
    29761 cgcaaaggca tccagtctgg catgctgacc gacggcgaga ataattttat ctgccgccat
    29821 cgtcagttcg cctgataacc gtacatgctt aaacgtgact ttattgcctt ccacggctac
    29881 tggcgtgaat ccatcgatga tcgaaacgcc cagctcccgg gcgctggcaa actctttttc
    29941 gcttgccgga aactcatcta actcttcacg tgctacgcaa gttaccgcct gacagccgag
    30001 aactttcagc gtgctggcta cgtccatcgc gacatcaccg ccgccgataa ttaatgcgct
    30061 ttgtggaatg ctgatatcgc cttgcgcctg tcgtacacgt tgcaggaagt cgacggcaat
    30121 ctcaacgtcg ctatgctcaa acagcggtag cccggaaccg cttgataacc ccacggtgac
    30181 cagtaccgcg cggttttccg ctttcagttg ctccagggtg agtgttttac cgatttcgtt
    30241 attgcactta atggtcacgc ccattttttc aatacgggcg atctctgcat ccagcactga
    30301 ttgcggtaac cggaattgcg gaataccgtt acgcaaccag ccaccggggt gcgcttcttt
    30361 ctcataaatc gtgacgtcat aaccctggtt tgtcagtgtc acactggcct gcaatccggc
    30421 aggacctgcg ccaataatcg cgactttgcc gagcgtttta gtaccgggct gataaatttc
    30481 catcccggtt tgttgttcaa aatcagtaac aaaacgctgt aagcggccga tatcaatggg
    30541 agcatcaaca ccggcacggg tacaaccgct ttggcataat ttctccgtcg ggcacactct
    30601 ggcacaaacg gcaccgaggg cattattttc gcgaattgtc tcggcagcgc ctttaaaatt
    30661 acgaaagtag attgagcgaa taaatttccc cggatcggtc tgtgccgggc aagcctgact
    30721 acagggagcg tcgtgacata ataaacagcg agaggcttct ttaatcgcca gtaacggcgt
    30781 aaaagccgga gtgagttcat ccagataatt ttgttgcggc atgtgatctt ccttaatgct
    30841 atgagtaatt atggcgaacg ctaaataaat attgcgtgct gatattaagg ctatgcgcgt
    30901 ggggaaattt aagctgtgaa aggattcaca aatagctgcg gttcagaatg ttatatccgc
    30961 agcttattga gtgaagtaat aattattctc aataattaac agaaaagaga aaatattaac
    31021 gatagaaaat aagataaaca atcatgccga tagataaaat tccgaagcca atatatgcca
    31081 ccatataagc gaagaagagt ccgggatttt ttgctcgcca gacatacacc ggttccggtg
    31141 catttttaac ccgtttatca atcgcgcctg cgcgccagcc tttccatgct tcgcggaaac
    31201 aaaatatcgg gataaggaaa aaaacggcaa cgacaaaaaa ctgctgtaca tccataacta
    31261 ctttccttgt ttcttttgta attcaagtaa ctgttcgggt gtaacggcgg gatacccctg
    31321 cgcatcttcc ggcacctggt gcatagccgg aatagggacc agcggcccta aaaaacgcgg
    31381 ttcacgttta aaaatataaa ggtcagccag cgcaccgaaa cgggcggcaa attcgcgaat
    31441 acgtactgac agcatatctt tcggtgacgg tacggcataa cactgagcct gaatccccat
    31501 atgcagcgca ataaataatg ctcgctcaca gtggaaacgt tgggtgataa taatgaaatc
    31561 attagtatcg aaaactttgc gtgtacgcac gatggaatcc agcgtacgaa agcctgcgta
    31621 atcgagaaca atatctgatg ggtcgacacc agcagcgatt aaatctttgc gcatggtcat
    31681 cggctcgtta taactttgca atgcgttatc gccgctcagt aatagataat ttaccttacc
    31741 gctgttatag gcattaatcg ctccttgaat gcggtagcga taatactgat taataacgcc
    31801 ggtacgataa tattttgctg tgccgagcac cacgccgacc tggcggtagg ggagatcctg
    31861 caattcgtcg tagatataag gcgcggtttt ccagctcatc cagcgatcga ggccgagcac
    31921 agtcaacagc agcaagccga tcaggactaa caggctgagg aacacgcgct ttaacatgca
    31981 ggagactcga gctaaggaaa atttccatca ggctacttta cccgctggtt ttgcgcaaga
    32041 aacggcaaac tgtttgccgg gaatttcgac accaggagag ggctggcaag ccggacaagg
    32101 cgttcacgcc gcatccggca ccaggcttaa gcgagaagca cgcggtcgat actgtgacag
    32161 ccgagagctt tcagcgttgc ggaggtggca tcccactgaa tcaacggcgc atcggctttt
    32221 tctgccagaa ccgcgcgctg gatatccggg taatcataac ccttgagatt caacagaatt
    32281 aacgccccct gcaacggcgg cagagtcggg ttgaatgcgg cgttttcagc gtagctgccg
    32341 ctgaaaatac gaccgtcttt acattccagc gcgacaccgc ttggtgactt actgtaaggc
    32401 atgtgcgaac ggtttgccgc tgcaatcgcc gcctgagaaa gcgcatcacc agtcagcgca
    32461 tagccgtgat cctgctcgtc catcagcagc gttttaatct ccagatcttt cggcccaaag
    32521 gcatccggca gatagtcacg cagcgcgtgt gcctcgcggc ccggcaaatg aatacgcaga
    32581 tccagaccgc tgttcagttc attcataaac tgacggcagt gaccacaagg cgtatagtta
    32641 acggtgatgg ctgcaagcgc tttttcacca ctcaaccaag cgtggctgat cgcgctttgt
    32701 tcggcatgaa cggtttgctg cattgttgca ccaataaatt ccatattggc accaaaatac
    32761 caggttccgc tcacaccgcg cgcaattgcg ccaacattaa aattcgacaa tggcgtacgc
    32821 gcacaggccg ccgccagcgg aagtagtgcg aatgccagcg cgtcttcgtc cagtcccgtt
    32881 gcgctcttca gcgatgagac ttgctccccg gtcaacaaag cggggaagta cttgtctgcc
    32941 agaataggtt ccagtgcaga ttgcaagtta tccgcaagtt gggcaaaagc ggtttgaaaa
    33001 cgtggatgca tggcgttgcc tcataatcat gtaatgggat aacagtttac gaacccgtta
    33061 tggctttata tgtgatctga atctcattaa ttatgcaaca cgtgaaactt acatcaaaaa
    33121 tgcgcgacgt atcgcaaatt ttagcccatt actgccagaa taatcgggaa aaggaacggt
    33181 gcgatcagcg aagtaattat cccgcataac accagcgcta gcgaactaaa tgcaccttcc
    33241 tgataatcca gctcggcgca gcgcgccgta ccgagggcgt gcgaggcagt ccccattgcc
    33301 agaccgcgcg cagctttggt acgaatacgc atcgcattaa gcaatgtatg gccaaatacc
    33361 gcgccgagga tgccgacgaa aatcacgcaa actgcgctga ttgccggaat accgccaata
    33421 ctgccgccaa ccgccattgc aataggcgtg gtgactgatt tcggcaggat tgacgcggcg
    33481 atttccggtg aagcgcccat caacaatgcc acggaagtcc ccgtcaccat cgcaaccacg
    33541 ctgccgataa aacagatggt gatgatcgat ttccagcgcg cgcggatctg gtgtagctgc
    33601 tcatataaag gataggccag cgcgacgacc gccggttgca gcagatcgtt tagcacttcg
    33661 ctacctttaa agtaactgtc gtaagagatg ccggtcagca ttaaaaaagg aatgatcacc
    33721 accattgcta ccagcaacgg gttaagcagg ggaaacttat accgtgccgc cagtttgcgg
    33781 gcggcaaaaa agacaatcaa cgtcagcggt aatgaccacc agatattcgc catcattctt
    33841 ctgatccttt ctgacctacg actttacgtt caccgtgtac cagttgtgaa ctccagctca
    33901 ccaccagaaa aaccaccagc gtactgacag cacaggaaac cactaccggg ccaaactgcg
    33961 cgcggagcaa atcaaaatat tgcatgacgc ctacgccaat cggcacaaac aatagcgcca
    34021 tatagcgaat cagtacgtag caccccggat tgacccattt cgccggaaga atttgcaagg
    34081 ccagcaggac aaacaggatc agcatcccga tgatgctgcc cggaatggtt accggtagca
    34141 gggaagcaat gaaaatgcct gcatacaggc aggcataaat caggacgaaa gcgcgtaaat
    34201 attgccagat aatgttcagt gtcttactca taatcaacac cccagatgag atggattcat
    34261 catacaatta attcgtaaaa cgtgctaccg atcacattaa gaaatcagcg cttatttttt
    34321 cgctacccgg tattacgcaa tgagcagcga actcaggcat acatcaagca tagagaggga
    34381 ttgcagtttg cagcaatccc tctttgcaga cctttaatcc gctgaccata ggcgctaaca
    34441 tagcgcctca tttttttgcg ggtgatgata tgcgtgtgtt actggcaccg atggagggag
    34501 tgcttgactc tctggtgcgt gaattgctga ccgaagttaa cgactacgat ctgtgcatca
    34561 ccgagtttgt ccgcgtggtg gatcaactgc tgccggtaaa agtctttcat cgcatttgcc
    34621 ctgagctaca aaacgccagc cggacaccat ctggtacgct ggtgcgcgtg cagttgttag
    34681 gtcagttccc acaatggctg gcagagaacg ccgcccgtgc ggtggagtta ggtttctggg
    34741 gcgtggatct caattgcggc tgcccgtcga aaacggttaa cggtagcggc ggcggggcga
    34801 cgttactcaa agatcctgaa ctcatctacc agggggcaaa agcgatgcgt gaagctgtac
    34861 cggcgcattt gcctgtcagc gtgaaagtgc gtctgggctg ggacagcggt gagaagaaat
    34921 ttgaaatcgc cgatgcggtt caacaggctg gcgctacgga gctggtggtg catgggcgga
    34981 cgaaagagca gggttaccgc gcggagcata ttgactggca ggcgattggc gagattcgcc
    35041 agcggctgaa tattccggtg attgccaacg gtgaaatctg ggactggcag agcgcgcaac
    35101 aatgcatggc gatcagcggc tgcgacgcag tgatgattgg tcgcggggcg ctcaatattc
    35161 ccaacctgag ccgggtggta aaatataacg aaccgcgaat gccgtggccg gaggtggttg
    35221 ctttgctgca aaaatatacc cgtctggaaa agcagggcga taccgggtta tatcacgttg
    35281 cgcggattaa acagtggttg agttatttgc gtaaagaata cgatgaagca acggaattat
    35341 ttcagcatgt tcgggtgttg aataactccc ctgatattgc aagggctatt caggcaattg
    35401 atatcgagaa actctaaaaa ccatagtgat gtttttgaca atgtgttagc tctttatcaa
    35461 taggccgcac ggttgtttgg tggcgtcaaa atcgccgagg cgttccctga aggccggggc
    35521 agcccacatg gatgtgggct gagggcgcgt tttacaggga tgttacctcg cgcccgaccc
    35581 ggtagccgta agggataagc cgagggcacc gcgcagcggc gattttgttc gccagagccc
    35641 gggggggcag ggggcggcgg cgattggtcg ccccctgcgc gctccttgcg ccagtgatta
    35701 tatgaagcaa tgcacatgac aggagcgcaa caggatgaga gactgaaata ttctcgttat
    35761 tttcggatct gatgacatta attaatttta aaatatcatc ttaaatacgc cagtcaccac
    35821 cagtagccca atcaggaaaa taatcaatac agcccagagt ataattttca ttggcgttcc
    35881 tttatttgtg agtgctgaat aagtatagga aaaaacgaag ccctttgccg ttatccataa
    35941 acgattaaga taaatcggaa gatatcattt acttagtcta tatttttctg ggtatgtcgt
    36001 tttattgcta ctgatgtatt gccacgcgaa actaaaaaat atatcgcaac acacggtaat
    36061 ctccgcgcac cgtttcttac ctgattattc ccccatgaat cgtgattcct tttatccagc
    36121 catcgcctgt tttccgctgt tactgatgct ggccgggtgt gcgcctatgc atgaaacccg
    36181 ccaggcgtta agccagcaaa cgcccgctgc acaagttgac accgcattac ccacggcgct
    36241 gaaaaatggt tggccagaca gccaatggtg gctggagtat cacgataatc agctcacttc
    36301 attaattaac aatgcactac agaacgcacc ggatatgcag gtggctgaac agcgcattta
    36361 gctagctgaa gcccaggcga aggcagttgc cactcaggat ggtccgcaga tcgacttttc
    36421 ggcggatatg gagcggcaaa aaatgtcggc agaagggcta atggggccgt ttgctctgaa
    36481 cgatccggcc gcaggtacga ccggtccgtg gtacaccaac ggaacttttg gcttaacggc
    36541 gggctggcat ctcgatatct ggggaaagaa tcgggcggag gttactgccc gcctgggtac
    36601 ggttaaagca cgggcggcgg aacgcgagca aacccgccaa ttgctggctg gcagcgtagc
    36661 ccgcctgtac tgggagtggc aaacccaggc ggcgttaaac acggtcttgc agcaaataga
    36721 aaaagagcag aacaccatta tcgcgaccga tcgccagcta tatcagaacg ggattacttc
    36781 ttcagttgac ggtgtggaaa ccgatattaa tgccagcaaa acccggcagc agctcaacga
    36841 tgtcgcgggg aaaatgaaaa ttattgaggc acggttaaat gcactcacaa atcaccagac
    36901 taagtcattg aagcttaaac cggtcgcgtt gccgaaagtg gcaagccagc ttcctgatga
    36961 actggggtac tccttactgg cccggcgggc agatttgcag gcggcgcact ggtacgttga
    37021 gtcatcgtta agcactattg atgcggcaaa agcggcattt tatcctgaca tcaacctgat
    37081 ggccttcctg caacaggatg cgttgcactt aagcgatctg ttccgtcatt ccgcgcagca
    37141 aatgggcgtt acggcaggcc tgacgctacc tattttcgat agtggtcgtc ttaacgccaa
    37201 tctcgatatc gcaaaagccg aaagcaactt gtctatcgcc agctacaaca aagcggtggt
    37261 tgaagcagtg aatgacgtgg cgcgggcagc cagtcaggtt cagacactgg cggagaaaaa
    37321 ccagcatcag gcgcaaattg agcgcgatgc cttgcgtgtg gtaggtcttg cgcaggcccg
    37381 ctttaacgcg ggcatcattg ctggttcccg cgtcagcgaa gccagaatcc cggcgctgcg
    37441 tgagcgggcc aatggcctgt tattgcaagg gcagtggctg gatgcctcca ttcgactcac
    37501 cggtgcgctg ggcgggggtt acaaacgctg atgatatgcg ccttctatac ttaacgttta
    37561 ttcagcgtta agtggagaac tcgatggcat aggtcgcgat aattaccgct tccgattcgg
    37621 ggatcggcaa agagtgcgcg ttattactgg cgcagcaggg gtttgatatt ggcattacct
    37681 ggcactcaga tgaagaaggg gcaaaagata ccgcgcgtga ggtggttacc cacggcgtac
    37741 gtgcggagat cgtgcaactg gatctcggca atctgccaga aggtgcgcag gcgctggaga
    37801 cactcatcca gcgatttggg cgtattgatg tgctggtgaa taatgcgggt gcaatgacca
    37861 aagcgccgtt tcttgatatg gcttttgatg agtggcgcaa gatttttacc gttgatgttg
    37921 atggtgcatt tttatgctcg caaattgcgg ctcgtcagat ggtgaaataa gggcagggcg
    37981 gtcgcatcat caacattacg tcggtacatg aacatacgcc gctgccggat gccagcgcct
    38041 acacagccgc taaacatgcg ctcggcgggt taaccaaaac gatggcgctg gagttggtca
    38101 ggcataagat tttggtgaac gcagtcgcgc ctggggcgat agccacgcca atgaacagca
    38161 tggatgatag cgacgtgaag cccgacgcgg agccttcgat tcctctacgg cgttttggcg
    38221 caacgcatga gattgccagc ctggtggtgt ggctttgttc ggagggcgca aattacacga
    38281 cagggcagtc gttgatagtg gatggcggct ttatgttggc gaatccgcag ttcaacccag
    38341 aatagcgcag tgaggtgcgt ggatatttca atgggttagg ccagataaga caccagcgtc
    38401 gcatcaggca tggaacaccc ggtgccggat gtggagtgaa tgcttatctg tggcaactgc
    38461 tttacgcctg attatccggc tttttcttcc cgcgccgttt cagccaccag cgcacgccca
    38521 ccaccagaac cacgaccaga atcaaccaga cccagtgctt caaatgctgg tcgagattgt
    38581 gcaaccacgg cgcaatcacc tgaccaccag cgtaaccaat agtggtaaaa atcaacgccc
    38641 aggcaaatgc gccgagaata ttcagcggca gaaagatttt cggcggcagc tggctggcac
    38701 caatcagcgt cgggccaatc acccgaaagc catacataaa gcgcgtacca atgacaaaca
    38761 gatacggatg gcgttggata agtttctgtg cccgctcaat tttatcctga tgtttcgaga
    38821 aacggcgtaa cagcttgccg ccaaaccgcc gcccgcacag atagagcacc tggtcgccaa
    38881 tcatgccacc aagcgccaca gaaagtacca ccagtgggaa ctttaatagc ccctgatgtg
    38941 ccgcaacgcc tcccagcaaa gtcacggttt caccttccgc caggctaccg ataaccagcg
    39001 cggcataacc atattgtgag atgagtgtat tgagatccat aggccagttt gtctcccttt
    39061 ggttgcctgt aagcatacac cctgtgaatc attcacgctg aaaaaggaca tacatcagac
    39121 ggtgcaaatt acatggatga acaaaaaata atcacaagct ggcttatact tgaactgtca
    39181 ggttcatgaa ctcacaacaa gggggcggta tgagccatgt ctggggactg ttttcccatc
    39241 ccgatcgtga aatgcaggtg attaatcgcg aaaacgaaac gatttctcac cattacaccc
    39301 accacgtttt gctgatggcg gcgattccgg tgatttgcgc cttcattggc actacacaga
    39361 ttggctggaa ttttggcgat ggcactatcc tgaagttatc ctggtttacc ggactggcgc
    39421 tggctgtctt gttttacggc gttatgctgg ctggggttgc ggtgatgggg cgggtcatct
    39481 ggtggatggc gcgcaattac ccgcagcgtc cgtctcttgc gcactgcatg gtcttcgcgg
    39541 gctacgttgc aactccgctg tttttaagcg gtctggtggc gctttatcca ctggtctggt
    39601 tgtgtgcgct ggtcggcacg gtggcgctgt tttacaccgg ctacctgctt taccttggta
    39661 ttccgagctt cctgaatatc aacaaagagg aaggccttag cttctccagt tcgacgctgg
    39721 cgattggcgt gctggtactg gaagtgctgc tcaccctaac cgttatacta tggggctacg
    39781 gttatcgttt gttctagaaa gttctttgac gttgcattgc tggcgtaaat gtcagcaatg
    39841 cagcatttac tcacgattct cctttgacga tcgcactttt tgctcgttat gatggcgtcg
    39901 ctagcctcag taaatcctta ctccggcggt gcgcaacccg tgcgcgtgaa ccactatctg
    39961 aatgctcatc atgccgaaat ttcgagtttc tttatttagc ttggccctga tgctggctgt
    40021 gccttttgca ccgcaggccg ttgcgaaaac ggcagccgct accaccgctt cacaaccgga
    40081 aattgcctcc ggtagcgcga tgattgttga tctgaatacc aacaaagtga tctattcgaa
    40141 ccacccggat ctggtgcgtc cgattgcgtc tatcagcaaa ttaatgaccg cgatggttgt
    40201 gctggatgca cgactgccgc tggatgaaaa actaaaagtg gatatcagcc agacgccgga
    40261 gatgaaaggg gtctattcgc gcgtacgact gaatagcgaa atcagccgta aagatatgct
    40321 gttgctggcg ctgatgtctt cagaaaaccg cgcggcggca agccttgcgc accattatcc
    40381 cggtggttac aaagccttta ttaaggcaat gaatgcgaaa gcgaaatcgc tcggaatgaa
    40441 caacacgcgc tttgttgaac ctaccggatt gtcggtgcat aacgtttcaa ctgcccgtga
    40501 cttaaccaaa ctgctcattg ccagcaaaca atatccgttg atcgggcagt taagtactac
    40561 ccgggaagat atggcaacct tctctaaccc gacgtatacg ctgccgttcc gcaatactaa
    40621 tcatctggtg tatcgcgata actggaatat tcagttaacc aaaaccggct ttaccaatgc
    40681 ggcgggccat tgtctggtga tgcgtacggt tatcaataat aaaccggtgg cgctggtagt
    40741 gatggacgcg tttggcaaat atacccattt tgccgatgcc agccgcctgc gtacctggat
    40801 tgaaaccggt aaagtgatgc ctgtgccggc agcagcgttg agctataaaa aacaaaaagc
    40861 cgcccaaatg gcggcggcgg ggcagacggc acagaacgat taagtgaatt cggatggcga
    40921 tactctgcca tccgtaattt ttactccact tcctgccagt ttttccgttt actggtttta
    40981 ccgatccccg gattcatgct attggtcgga tcattctcgc gatagaactt ctgcaacgtc
    41041 tccggtgctt tatacaaatg accgacgtta tgctcggcag ggtactgcgc gccgcgctgc
    41101 tgtagcagtt ccagcatctg ttcttttaac gcatgcacat ccacgccttt tttcactatg
    41161 taatcctgat ggaagacata gcacataaaa tggccgtaat agagcttgtg caccagctgg
    41221 ctgtcgatct ccggcggtaa atgctcatac cactcggtgt cgttacgccg cagagcgata
    41281 tccagtgcca gaatgtcttc gacttcatcg gaatgcaccg cctgataacg aattgctgcg
    41341 cccgcagcgg cgaaacggtg taaaaacgct ttgctacctt cctccggcgt acagacaaag
    41401 aaatcgccct cggcctgttt gaaataatcc accagccacg atttggcttc accgacgcca
    41461 tcgcctgcca tttttaacag cagatgatgc tcgtatttat cgcgccagtt tttcatgcgc
    41521 ggcggtaaat ggctggggaa caggtgaccg aatttttgca ttgcacggtc agtaaaatgc
    41581 ggacggaaga atttcacttt ctccagcatc gcatcggtgc gtcccttgag attaaagaag
    41641 aacggcatct tgtcggtgcc taacttatca atcatcagga aggtgtcttt gccgtatttt
    41701 tccgcaatat cgtagatatc ccggtgcata tattccccgg caaccggcag attttcgaag
    41761 ttagccagaa tatgacggcg gatttcggtc agcacttccg gctggttggt gccgatataa
    41821 aacacctgct gatttttttc cgcttcgaag gtatcaagac gtaccgcaaa gacggccagc
    41881 ttcccggcgc aaccagaaga ttcaaataac cgatccggat cggcgttata acgtgcgggc
    41941 gtgtcggctt caatatcacg aacgcggtgg acatagtcat aatcgtgggc gtgacgacca
    42001 tcgtgacgca catcgtcatc tttgatgcga tcgtcatcca gcttgctaag gatctgctcc
    42061 ggcgtttcgc ccagatcaat ccccagatgg ttcaccagcg tcagtttgcc atcttcatct
    42121 atacgcgcga ataacgacat ttcggtatac gccgggccac gttgcaccag cgagccgccg
    42181 gagttgttac aaataccgcc gatgaccgat gcgcctatac acgatgatcc aatcaccgaa
    42241 tgcggttcgc gtcccagcgg tttgagggct ttttccagcg aatagagcgt ggtgcccgga
    42301 taggccagca cctgttcgcc cttgccgaga acgtgcagct tgtcgagacg cagggtgctg
    42361 atgatcacga tatcgcgatc atagtcgtta ccgtttggcg tcgatccttc ggtcaggcct
    42421 gtattggcag cctgcatcag aataattttg tcggcggtga cgcaggcttt cagcacccgc
    42481 cacaattcca gtagtgagcc agggaaaacg acagccagcg cgtcgccctg accagaacgg
    42541 aagcccttgc gatagcgggc cgtttttgcg ggatcggtga gcaggtgtga atgaccgacc
    42601 agacgggcaa gttcattcaa aaaggcttta ttatcagttg ttgtcatgga agacattttc
    42661 cactccttgt ggtggcgaaa aaatatcaca aactagaata gcgccttgta tgacaattca
    42721 gcgagtgaat attttgaaaa ttcagacaat aaactgagca ttctttttct ctctccatca
    42781 tgcttatggc aaactacggt ttttgcacca ttttcgctgc cacgtcgggc aacaaaggaa
    42841 gaaaaatcca tatgaaatgg ctatgttcag taggaatcgc ggtgagtctg gtcctgcagc
    42901 cagcactggc ggatgattta ttcggtaacc atccattaac gcctgaagcg cgggatgcgt
    42961 tcgtcaccga tctgctcaag aaaatgacgg ttgatgagaa aattggtcag ctgcgcttaa
    43021 tcagcgtagg cccggataat ccgaaagagg cgatccgcga gatgatcaaa gacggtcagg
    43081 ttggggcgat tttcaacacc gtaacccgtc aggatatccg cgccatgcag gatcaggtga
    43141 tggaattaag ccgcctgaaa attcctcttt tctttgctta cgacgtgctg cacggtcagc
    43201 gcacggtgtt cccgattagc ctcggtctgg cctcgtcttt taacctcgat gcggtgaaaa
    43261 cggtcgggcg tgtctctgct tatgaagcgg cagatgatgg cctgaatatg acctgggcac
    43321 cgatggtcga tgtctcgcgc gatccgcgct ggggacgtgc ttccgaaggt tttggcgaag
    43381 atacgtatct cacctcaata atgggtaaaa ccatggtgga agcgatgcag ggtaaaagcc
    43441 cggcagatcg ttactcggtg atgaccagcg tcaaacactt tgccgcatac ggcgcggtag
    43501 aaggcggtaa agagtacaac accgtcgata tgagtccgca gcgcctgttt aatgattata
    43561 tgccgccgta caaagcgggg ctggacgcag gcagcggcgc ggtgatggtg gcgctgaact
    43621 cgctgaacgg cacgccagcc acctccgatt cctggctgct gaaagatgtt ctgcgcgacc
    43681 agtggggctt taaaggcatc accgtttccg atcacggtgc aatcaaagag ctgattaaac
    43741 atggcacggc ggcagacccg gaagatgcgg tgcgcgtggc gctgaaatcc ggaatcaaca
    43801 tgagtatgag cgacgagtac tactcgaagt atctgcctgg gttgattaaa tccggcaaag
    43861 tgaccatggc agagctggac gatgctgccc gccatgtact gaacgttaaa tatgatatgg
    43921 ggttgtttaa cgacccatac agccatttgg ggccgaaaga gtctgacccg gtggatacca
    43981 atgccgaaag ccgcctgcac cgtaaagaag cgcgtgaagt ggcgcgcgaa agcctggtgt
    44041 tgctgaaaaa ccgtctcgaa acgttaccgc tgaaaaaatc ggccaccatt gcggtggttg
    44101 ggccactggc ggacagtaaa cgtgacgtga tgggcagctg gtccgcagcc ggtgttgccg
    44161 atcaatccgt gaccgtactg accgggatta aaaattccgt cggtgaaaac ggtaaagtgc
    44221 tgtatgccaa aggggcgaac gttaccagtg acaaaggcat tatcgatttc ctgaatcagt
    44281 atgaagaagc ggtcaaagtc gatccgcgtt cgccgcaaga gatgattgat gaagcggtgc
    44341 agacggcgaa acaatctgat gtggtggtgg ctgtagtcgg tgaagcacag gggatggcgc
    44401 acgaagcctc cagccggacc gatatcacta ttccgcaagg ccaacgtgac ttgattgcgg
    44461 cgctgaaagc caccggtaaa ccgctggtgc tggtgctgat gaacgggcgt ccgctggcgc
    44521 tggtgaaaga agatcagcag gctgatgcga ttctggaaac ctggtttgcg gggactgaag
    44581 gcggtaatgc aattgccgat gtattgtttg gcgattacaa cccgtccggc aagctgccaa
    44641 tttccttccc gcgttctgtc gggcagatcc cggtgtacta cagccatctg aacaccggtc
    44701 gtccgtataa tgccgacaag ccgaacaaat atacttcgcg ttattttgat gaagctaacg
    44761 gagcgcttta tccgttcggc tatggtctga gctataccac tttcaccgtc tctgatgtga
    44821 aactttctgc gccgaccatg aagcgtgacg gcaaagtgac ggccagcgtg caggtgacga
    44881 acaccggtaa gcgcgagggg gccacggtag tgcagatgta cttgcaggat gtgacggctt
    44941 ccatgagtcg ccctgtgaaa cagctgaaag gctttgagaa aatcaccctg aaaccgggcg
    45001 aaactcagac tgtcagcttc ccgatcgata ttgaggcgct gaagttctgg aatcaacaga
    45061 tgaaatatga cgccgagcct ggcaagttca atgtatttat cggcactgat tccgcacgcg
    45121 ttaagaaagg cgagtttgag ttgctgtaat gctttagtaa gggcgcgacg tttgcgccct
    45181 ttgcctgatg ttcatatctt aacttgctga attttcgccc tttgtaggcc ggataaggcg
    45241 ctcgcgccgc atccggcagg aaacgccgga ccgaatatgc ctcttaagta tttatctgat
    45301 tctgcaactg aatccttccg ctcaggctaa ctctgtcatt atcaactatg cttttctctt
    45361 aattcgctga aaacaacagt aaaagaatga ggaaagcatc atgccactct taaagctctg
    45421 ggcaggttca ctggttatgt tggcagccgt gagcctgccg ctacaggcgg cttcccccgt
    45481 taaagtcggt tcaaaaatcg ataccgaagg cgcgctgctc ggcaacatca tcttgcaggt
    45541 actcgaaagc cacggcgtac cgacggtgaa taaagtgcaa ctcggaacga ctcctgtggg
    45601 gcgcggggcg attacttccg atgaactgga tatctatccg gaatataccg gcaatggcgc
    45661 tttcttcttt aaagatgaaa acgatgcagc gtggaaaaat gccgggcagg gctacgagaa
    45721 agtcaaaaag ctcgatgcgg agcacaacaa gttaatctgg ctgacgcccg cgcctgcaaa
    45781 taacacctgg actatcgccg tgcgtcagga tgtggcagag aaaaacaaac tcacttcgct
    45841 tgccgacctg agtcgttatc tgcaagaggg cggcaccttc aaactggcag cctcggcaga
    45901 gtttatcgaa cgcgccgatg cgttacccac gtttgaaaaa gcctatggct ttaagctcgg
    45961 tcaggatcag ttgctgtcac tggctggtgg cgacacggcg gtgacgatta aagccgctgc
    46021 ccagcaaacc tctggcgtta atgccgcaat ggcttacggc actgacggtc cggtcgcggc
    46081 gctggggctg caaaccttaa gcgatccgca aggcgtgcaa cctatctacg cgcctgcacc
    46141 agtggtgcgt gaatcggtgt tgaaggagta tccgcaaatg gcacagtggc tacagccagt
    46201 cttcgccagc ctcgatgcaa aaacattgca gcaactgaat gccagcattg cagtggaagg
    46261 actggatgcc aaaaaagtgg ctgccgacta cctgaaacaa aaagggtggg cgaagtaatt
    46321 tcccgtgact tatctacgta ttaatcctgt tctggcgctg ctgttgttgc tgacggcaat
    46381 cgcagcggcg ctgccgttta tcagttacgc gcctaatcgt ttagtctcgg gtgaagggct
    46441 tcatctctgg caactgtggc cgcaaacgat ctggatgctg gtgggcgttg gttgcgcctg
    46501 gctgacagcc tgttttgtcc ccgctaaaaa aggcagcatt tttgcactca ttctggcgca
    46561 attcgtcttc gtattgttgg tgtggggagc tggaaaggcg gcgacccaac tggcgcaaaa
    46621 tggcagtgcg ctggcgcgta ccagcctcgg cagaggtttc tggctggctg cggcgctggc
    46681 attgctggcc tgtagcgatg ccatccgccg aatctccacg catccgctgt ggcgctggtt
    46741 gttgcatatg cagattgcca ttattccgct gtggttgctg tactccggca cgcttaacga
    46801 tctctcacta atgaaagaat acgccaaccg tcaggatgtg tttgacgacg cgctggcaca
    46861 acatctgacg ttgctgtttg gtgcggtgct gcctgcgtta gtgattggtg tgccgttggg
    46921 catctggtgc tacttttcca ctgcgcggca gggggcaatt ttttctctgc tcaatgtcat
    46981 tcagaccgtg ccttcggtgg cgctctttgg cctgttgatt gcgccgcttg ccgcgctggt
    47041 tacggccttt ccgtggctgg ggaagctcgg catagcaggg accggaatga cacccgcact
    47101 gattgcgctg gtgctctatg ccttgctgcc gctggtgcga ggcgtggtag tcggtttgaa
    47161 ccagatcccg cgcgatgtgc tggagagcgc cagagcgatg gggatgagcg gggcgcagcg
    47221 tttcctgcat gttcagttac cgctggcgtt accggtattt ctgcgcagcc tgcgggtggt
    47281 gatggtgcaa actgtaggta tggcggtgat tgcggcgtta atcggcgcag gcggttttgg
    47341 tgcgctggtt ttccaggggc tgctaagcag cgccattgat ttagtgttgc tgggggtgat
    47401 cccggtaatt gttctggcgg tgcttaccga cgcgctgttc gatttgctta tcgcactgct
    47461 gaaggtgaaa cgtaatgatt gaatttagcc atgtcagcaa actgttcggc gcacaaaaag
    47521 ccgttaacga tctcaatctc aattttcagg aagggagttt ttcggtgctg attggcacat
    47581 ctggctccgg caaatccacc accctgaaaa tgattaaccg cctggtggag catgacagcg
    47641 gagtgatccg ctttgccgga gaagaaattc gctcgctgcc agtactggag ttgcgccgcc
    47701 ggatgggcta tgccattcaa tctattggcc tgttccccca ctggagcgtg gcgcaaaaca
    47761 tcgctaccgt gccgcaatta caaaaatggt cgcgggcgcg gattgacgat cgtatcgacg
    47821 aattaatggc gctactgggg ctggagtcaa atttgcgtga gcgttatccg catcagcttt
    47881 ccggtggtca gcagcaacgt gtgggagtgg cgcgtgcact ggctgccgat ccgcaagtct
    47941 tactgatgga tgaacctttt ggcgcactgg acccggtaac gcgcggcgcg ttgcaacaag
    48001 agatgacgcg cattcaccgt ttgctggggc gcactattgt gctggtcact catgatattg
    48061 atgaggcgct acggctggca gaacatctgg tattgatgga tcacggtgaa gtggtgcagc
    48121 aggggaatcc gctgacgatg ctgactcgtc cggcgaatga ttttgtccgc cagttttttg
    48181 gacgtagtga actgggggtg cacctgcttt cgttacgtag cgtggcggat tacgtgcgtc
    48241 gcgaagaacg ggcagaaggt gaggcactgg cagaagagat gacgctacgc gatgcgctct
    48301 ccctgtttgt cgcgcgggga tgcgaggtgc tgccggtggt gaacacgcag ggccagcctt
    48361 gcggcacgct gcattttcag gatctgctgg tggaggcgta agcgtatgaa gatgttgcgc
    48421 gatccgctgt tctggctcat tgctcttttt gtggcgctga ttttctggct gccttacagc
    48481 cagccgctgt ttgctgcctt gttcccacaa ctgccacgac ccgtttatca gcaagaaagt
    48541 tttgcagctc tggcactggc tcatttctgg ctggtgggaa tttcgagttt gtttgcggtg
    48601 atcattggca caggtgccgg aattgctgtt actcgcccgt ggggcgcgga atttcgccca
    48661 ctggtggaaa ctattgccgc cgttggacag acttttccgc ctgtcgcagt gctggcgatt
    48721 gccgttccgg tgatcggctt tggtctgcaa ccagcgatta tcgccttgat cctttacggt
    48781 gtgctgccca tcctgcaggt gacacttgcc gggctgggag cgattgatcc cagcgtgaca
    48841 gaagttgcga aaggtatggg aatgagtcga ggtcagcgac tgcgtaaggt cgagctaccg
    48901 ctggcggctc cggtgattct ggcgggcgtg cgaacttcgg tgattatcaa cattggtacg
    48961 gcgacgatcg cctcaacggt aggggccagt acgctgggta cgcccatcat catcgggctt
    49021 agcggattta ataccgcgta tgtgatccag ggggcgttac tggtggcact ggcggcgatc
    49081 atcgcagacc gcctgtttga aaggctggtg caggcgctta gccagcacgc aaaataaagg
    49141 tataacctgc gagcatgacg ccaccaattc cgcctaacgc cataaacagg aacagggcga
    49201 tgaccccaat tttagctatg cgcataatgc actccttatg ttaacgaaag gattgtacag
    49261 taaagcgcat ttgttaacga atcattaaat accgagtggg aaaatatcat ggccttgttc
    49321 ttgccaactg gtgagttgct gctgttgggc ggaggttcga ttttcaccgc accacaccag
    49381 caatgtacgg ccttcgaata gttcagggcg tagttgattg agcgagtggg cgagcgagga
    49441 catcaatgcg ccatccttgt tgactggcaa tccagccctc cagccacaga cgggtggtat
    49501 cctgaatatt ccagccaacc accagcgcat ctttaccctg ttttttacgt gccgaagcca
    49561 gacaaatggc gatgtagttg atcagtacgc cgtcgaggat cgccagcagc gcctggagag
    49621 tcggttgttg gcactgaagc cgtcggcgca gaggaataaa cagatgtgtg gtgagtgtct
    49681 gggcggggta atcctgaccg cgctctttga tccacgttcg caggctatgt agattgccg
//
LOCUS       DAEBXN010000021        47797 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.021, whole genome shotgun sequence.
ACCESSION   DAEBXN010000021 DAEBXN010000000
VERSION     DAEBXN010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47797
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.021"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            75..227
                     /gene="hokA"
                     /locus_tag="KJL99_001186"
     CDS             75..227
                     /gene="hokA"
                     /locus_tag="KJL99_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HBD4435980.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     AYKLKE"
     gene            complement(414..626)
                     /gene="cspA"
                     /locus_tag="KJL99_001187"
     CDS             complement(414..626)
                     /gene="cspA"
                     /locus_tag="KJL99_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HBD4435981.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(907..1197)
                     /locus_tag="KJL99_001188"
                     /pseudo
     CDS             complement(907..1197)
                     /locus_tag="KJL99_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
     gene            1631..2341
                     /locus_tag="KJL99_001189"
     CDS             1631..2341
                     /locus_tag="KJL99_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HBD4435982.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2391..3365)
                     /gene="ghrB"
                     /locus_tag="KJL99_001190"
     CDS             complement(2391..3365)
                     /gene="ghrB"
                     /locus_tag="KJL99_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HBD4435983.1"
                     /translation="MKPSVILYKALPDDLLQRLQAHFTVHQVANLSPQTVVQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRVHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3469..4119)
                     /locus_tag="KJL99_001191"
     CDS             complement(3469..4119)
                     /locus_tag="KJL99_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HBD4435984.1"
                     /translation="MFILLAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSLVGA
                     GIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRSGDN
                     IILNMPNNVTFDSSSAPLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDLNMR
                     LSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSPL"
     gene            4282..6615
                     /gene="bisC"
                     /locus_tag="KJL99_001192"
     CDS             4282..6615
                     /gene="bisC"
                     /locus_tag="KJL99_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HBD4435985.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTTLAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6584..7024)
                     /locus_tag="KJL99_001193"
     CDS             complement(6584..7024)
                     /locus_tag="KJL99_001193"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBD4435986.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7021..7584)
                     /gene="tag"
                     /locus_tag="KJL99_001194"
     CDS             complement(7021..7584)
                     /gene="tag"
                     /locus_tag="KJL99_001194"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HBD4435987.1"
                     /translation="MERCGWVSQGPLYIAYHDNEWGVPETDSKKLFEMICFEGQQAGL
                     SWITVLKKRENYRAYFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFPDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYLGNKP"
     gene            7742..8440
                     /locus_tag="KJL99_001195"
     CDS             7742..8440
                     /locus_tag="KJL99_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBD4435988.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8669..9877
                     /locus_tag="KJL99_001196"
     CDS             8669..9877
                     /locus_tag="KJL99_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD4435989.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTRSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            10201..11892
                     /gene="eptB"
                     /locus_tag="KJL99_001197"
     CDS             10201..11892
                     /gene="eptB"
                     /locus_tag="KJL99_001197"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HBD4435990.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LVVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            11984..12060
                     /locus_tag="KJL99_001198"
     tRNA            11984..12060
                     /locus_tag="KJL99_001198"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12018..12020,aa:Pro,seq:cgg)
     gene            12804..14411
                     /gene="dppA"
                     /locus_tag="KJL99_001199"
     CDS             12804..14411
                     /gene="dppA"
                     /locus_tag="KJL99_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HBD4435991.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            14719..15738
                     /gene="dppB"
                     /locus_tag="KJL99_001200"
     CDS             14719..15738
                     /gene="dppB"
                     /locus_tag="KJL99_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HBD4435992.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHVQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            15748..16650
                     /gene="dppC"
                     /locus_tag="KJL99_001201"
     CDS             15748..16650
                     /gene="dppC"
                     /locus_tag="KJL99_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HBD4435993.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVAIVLF
                     IAIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            16661..17644
                     /gene="dppD"
                     /locus_tag="KJL99_001202"
     CDS             16661..17644
                     /gene="dppD"
                     /locus_tag="KJL99_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HBD4435994.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            17641..18645
                     /gene="dppF"
                     /locus_tag="KJL99_001203"
     CDS             17641..18645
                     /gene="dppF"
                     /locus_tag="KJL99_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HBD4435995.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFVPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(18675..19946)
                     /locus_tag="KJL99_001204"
     CDS             complement(18675..19946)
                     /locus_tag="KJL99_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HBD4435996.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPVTWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLIAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            20422..20529
                     /locus_tag="KJL99_001205"
     CDS             20422..20529
                     /locus_tag="KJL99_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HBD4435997.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            20905..21012
                     /gene="ldrD"
                     /locus_tag="KJL99_001206"
     CDS             20905..21012
                     /gene="ldrD"
                     /locus_tag="KJL99_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxic polypeptide
                     LdrD"
                     /protein_id="HBD4435998.1"
                     /translation="MTFAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            21388..21495
                     /locus_tag="KJL99_001207"
     CDS             21388..21495
                     /locus_tag="KJL99_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HBD4435999.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            21871..21978
                     /locus_tag="KJL99_001208"
     CDS             21871..21978
                     /locus_tag="KJL99_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HBD4436000.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(22065..23744)
                     /gene="bcsG"
                     /locus_tag="KJL99_001209"
     CDS             complement(22065..23744)
                     /gene="bcsG"
                     /locus_tag="KJL99_001209"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HBD4436001.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDIWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIDFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(23741..23932)
                     /gene="bcsF"
                     /locus_tag="KJL99_001210"
     CDS             complement(23741..23932)
                     /gene="bcsF"
                     /locus_tag="KJL99_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HBD4436002.1"
                     /translation="MMTISDIIEIIIVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(23929..25500)
                     /gene="bcsE"
                     /locus_tag="KJL99_001211"
     CDS             complement(23929..25500)
                     /gene="bcsE"
                     /locus_tag="KJL99_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HBD4436003.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            25773..25961
                     /locus_tag="KJL99_001212"
     CDS             25773..25961
                     /locus_tag="KJL99_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HBD4436004.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            25973..26725
                     /gene="bcsQ"
                     /locus_tag="KJL99_001213"
     CDS             25973..26725
                     /gene="bcsQ"
                     /locus_tag="KJL99_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HBD4436005.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCEHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            26722..27081
                     /locus_tag="KJL99_001214"
     CDS             26722..27081
                     /locus_tag="KJL99_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HBD4436006.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYTHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     SGHFQVCKIFVDVTING"
     gene            27078..29339
                     /gene="bcsA"
                     /locus_tag="KJL99_001215"
                     /pseudo
     CDS             27078..29339
                     /gene="bcsA"
                     /locus_tag="KJL99_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077779.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            29350..31689
                     /gene="bcsB"
                     /locus_tag="KJL99_001216"
     CDS             29350..31689
                     /gene="bcsB"
                     /locus_tag="KJL99_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HBD4436007.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGEQGADAPIVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNFEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLATISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            31696..32802
                     /gene="bcsZ"
                     /locus_tag="KJL99_001217"
     CDS             31696..32802
                     /gene="bcsZ"
                     /locus_tag="KJL99_001217"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HBD4436008.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYMPPTLAQYFTRFGAPWTTLRETNQHLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDTYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            32879..33220
                     /locus_tag="KJL99_001218"
     CDS             32879..33220
                     /locus_tag="KJL99_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HBD4436009.1"
                     /translation="MDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRRSIDDIER
                     SLQNDRLAQQAEVLENQGKWAQAAALQRQRLALGPGSVWITYRLAGRSCAAIQRWRQQ
                     SGLWLHGAGIT"
     gene            33135..33386
                     /locus_tag="KJL99_001219"
                     /pseudo
     CDS             33135..33386
                     /locus_tag="KJL99_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BCSC C-terminal domain-containing protein"
     gene            33468..35456
                     /gene="hmsP"
                     /locus_tag="KJL99_001220"
     CDS             33468..35456
                     /gene="hmsP"
                     /locus_tag="KJL99_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HBD4436010.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            35643..36929
                     /gene="dctA"
                     /locus_tag="KJL99_001221"
     CDS             35643..36929
                     /gene="dctA"
                     /locus_tag="KJL99_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HBD4436011.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGALGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            37150..38646
                     /locus_tag="KJL99_001222"
     CDS             37150..38646
                     /locus_tag="KJL99_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBD4436012.1"
                     /translation="MQGIKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASDSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(38742..39671)
                     /gene="kdgK"
                     /locus_tag="KJL99_001223"
     CDS             complement(38742..39671)
                     /gene="kdgK"
                     /locus_tag="KJL99_001223"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HBD4436013.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLASEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRAKG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEALVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            39903..40670
                     /gene="pdeH"
                     /locus_tag="KJL99_001224"
     CDS             39903..40670
                     /gene="pdeH"
                     /locus_tag="KJL99_001224"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HBD4436014.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            40740..42800
                     /locus_tag="KJL99_001225"
     CDS             40740..42800
                     /locus_tag="KJL99_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HBD4436015.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGNLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYIFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(42982..44304)
                     /locus_tag="KJL99_001226"
     CDS             complement(42982..44304)
                     /locus_tag="KJL99_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HBD4436016.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(44715..45728)
                     /gene="yhjD"
                     /locus_tag="KJL99_001227"
     CDS             complement(44715..45728)
                     /gene="yhjD"
                     /locus_tag="KJL99_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HBD4436017.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(45777..46676)
                     /locus_tag="KJL99_001228"
     CDS             complement(45777..46676)
                     /locus_tag="KJL99_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBD4436018.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            47196..>47797
                     /locus_tag="KJL99_001229"
     CDS             47196..>47797
                     /locus_tag="KJL99_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBD4436019.1"
                     /translation="MQIVMFDRQSIFIHGIKISLQQRIPGVSIQGVSQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
BASE COUNT        11444 a        11909 c        12859 g        11585 t
ORIGIN      
        1 agtgaggcta ccctatgcct gaacaccata aggttagcct cttactcgtt ggaaatcaac
       61 acagggggct gggaatgccg cagaaatata gattactttc tttaatagtg atttgtttca
      121 cgcttttatt tttcacctgg atgataagag attcactgtg tgaattgcat attaaacagg
      181 agagttatga gctggcggcg tttttagcct acaaattgaa agagtaagag tcttcgacgg
      241 gaaattattc ccgccttact tacggcgttg cgcattctca ttgcacccat atttattctt
      301 cacaaaaata ataatagatt ttattacgcg atcgattatt tatttcctga aaaaaaataa
      361 aaaaatcccc gccaaatggc agggatctta gattctgtgc ttttaagcag agattacagg
      421 ctggttacgt taccagctgc cgggccttta gcgccgcttt cgatggtgaa ggacactttc
      481 tgaccttcgt ccagagattt gtaaccatcg ttctggatag cagagaagtg tacgaacaca
      541 tctttagagc catcgtcagg agtgatgaag ccgaagcctt tgtcagcgtt gaaccatttt
      601 acgataccag tcattttacc ggacatagtg tattaccttt aataattaag tgtgcctttc
      661 ggcgatatgg cgtgctttac agattttgaa gcgttaaagg aatgtgcact acgaggggta
      721 tcaacgataa ctcttgaagg gacttgcctt actacactgc tttaatggtc tgtacgtcaa
      781 accgttgatg tgcattaagc cactcattgg cgggtgatgc aacaattatt tttcatattt
      841 atgattaatc ggttggaaaa cggtcctgtc atcaggaccg taaacagcaa taaagtggat
      901 aaaagtctat ttcatcaact gcttacttaa tgccgggttg gcttgaatca aacgcatcaa
      961 ttttagttcg gcacttgaag gcttcacgcg tctggattcc cattccttta ccatggcgac
     1021 tgatacgccc aaaacccggg cgaaatcatc gatttttaat cctgtccctt ttcataactg
     1081 ctcaatttcg gtacaggacg ttgttctgtg cgtcagggta attttctgcg tttcatcttt
     1141 aaaaacaatc tgttccaggc tgctcaacag ctcatgcatt ggatctttat attccattga
     1201 aaactcctca aatcacactg cgggatcgtg aattgcatcg aagctcatta agaatagtcg
     1261 ggaaaagcag acacagcggg gtgaagtcag ggcatgctct ggctatgatt cgattcagcc
     1321 tgtcatttag cgtgacttat agccacctaa cgatctgata aatgccgata tatctcttat
     1381 cactcctgtt ttgtaaagat ttcgcaaatc taaccttctg cgaaggcgta aatctctggc
     1441 tacaaaaaag gcaacggcac tatcattttt taacaatgaa acaacaaatt aaccttgttt
     1501 cattttctcc atgattttac caggaagatt acgtagaaag ggtatcctgc aagcctgtcc
     1561 tggactatcc ttgtcacgtc agacacgcgt gtgtcgttgt gcgctttttt tgggtgaaag
     1621 gagtaagaaa atggcgacag gaaagtcctg ctctcgctgg tttgcgcctc ttgcggcgtt
     1681 attaatggta gttagcctga gtgggtgttt tgataaagaa ggcgatcagc gtaaagcgtt
     1741 tatcgacttc ctgcaaaata cagtgatgcg tagcggtgaa cgtctaccaa ccctgactgc
     1801 cgatcagaaa aaacagtttg gtccttttgt ctctgattac gcgattctgt atggttattc
     1861 tcagcaggtg aatcaggcga tggattccgg tctgcgcccg gtcgtagaca gcgttaacgc
     1921 aattcgcgta ccgcaggatt acgttacgca aagcggtccg ctgcgtgaaa tgaatggttc
     1981 gctgggtgtg ctggcgcaac aactgcaaaa tgcgaagttg caggccgatg ctgcgcactc
     2041 tgcgctaaaa cagagcgatg acctgaaacc ggtatttgat caggcattca ccaaagtggt
     2101 aacgacgcca gctgatgcat tgcaaccgtt gattccggcg gcacaaactt tcacgcaaca
     2161 actggtgatg gttggagact acattgctca gcaaggtact caggtgagct ttgtcgcaaa
     2221 tggcatccag ttcccgactt cacagcaggc aagtgaatat aacaaactga ttgcgccatt
     2281 accggcacaa catcaggcgt ttaatcaggc ctggactaca gcagttactg caacccaata
     2341 aagagtaaaa gcccgagcga ttatcgggct ttacttcacg cagtcgcagc ttagtccgcg
     2401 acgtgaggat tcacacagtt cttctcaacc tttccttgta acgcatcaat caaattatcc
     2461 acggcacagg cggccatgcc ataacgcgtc tcatgggtgg cagatccaat atgcggtact
     2521 gcgacgacgt tggccattga gagcaacggc gaatctacgg acagtggctc ttgttcgaag
     2581 acatccagcc cggcggcgtg aatttcgcct ttctgcaatg ctgcgatcag tgcattttcg
     2641 tcaaccaccg ggccacgtcc ggcattaatg aaaatggcgg aggatttcat tttggcgaat
     2701 tgttctgcgc caaacagatg atgcgtctca tcagttaacg gcaggatcag gcaaacgaaa
     2761 tctgactctt gtaacagagt atccaaatcg cagtagcggg cgttgaagcg ttcttctgct
     2821 tctttatggt ggcggcgcgc gttatagagg atgggcatgt tgaagccaaa gtgcacacgt
     2881 tgtgccagcg ccatgccgat ccgtcccatc ccgacaatgc ccagtgtttt atggtgaacg
     2941 tcagtgccgt accagtccgg gcctatgctc gcggtccatt cgcctgcttt tacccgttct
     3001 gctacctcca caacccgacg agcggtagac aacaccagcg ccatcagcgt atcggcgacg
     3061 gtttctgtta atacggttgg cgtgtgcatc agcagaattt ttcgggcggt aagcgcatcg
     3121 acatcaaaat tgtcatagcc gacggagatc gttgatgtgg cacgcagttt cggcattttt
     3181 tccagcaatg cggcatctac attctcgttt gaacccagta aaccttcagc ttctgcaaaa
     3241 attgctgcat tttgaacgac ggtttgtggg ctgaggtttg ccacctggtg aacggtgaaa
     3301 tgcgcttgca ggcgttgcag taaatcatca ggtaaggctt tgtagaggat aacggacggc
     3361 ttcatgcttc tctccattca ctgataaggc gatcacctta gcctgaaata tgatgaagga
     3421 aaactgaaga atgccaggtg atttctgcat cacctggcat gaaagggatt acagcgggct
     3481 taaggtaatt tctacacggc ggttttgcgc cttaccttct gcggtgctgt tgctggcgat
     3541 tgggttagcc gggccaaggc cctgagtacg gatgcggctg gcgtccacgc cctgggtgat
     3601 caacgcgctg gcaacggaat ccgcacgctg ctgggagaga cgcatgttca ggtcgtgacc
     3661 ccccgtgctg tcggtataac caatcacgtt aaccgccgtt ttcggatact ctttcagcac
     3721 cattgccacg ccggtcaggg tgttagcgcc tgccggtttc aggggagcgc tgctgctgtc
     3781 gaaggtcaca ttgttcggca tattgaggat aatgttatcc ccgctgcggg ttacgctaac
     3841 accagtgccg cgcattttgt cacgcagctt cgcttcctgc acatccatgt aataacccac
     3901 gccgccgccc agagctgcgc ctgctgctgc gccaatcagc gcgcctttac cgcgatcttt
     3961 cttcgaagaa gagagcgcac caatacccgc gcccacgaga gagcccagac ctgcgccgat
     4021 agcagatttg cctgcttcgc gttcgccggt gtaagggtta gttgtgcagc cagataccgc
     4081 cagagcgcca ctcactacgg cggcaagtaa gataaacacg tttcttcatt gttaatcctt
     4141 aataaccttt ttattctttg tcacgggttc cgtggcggga gattatgccg cgtgaacatg
     4201 aagattattc ctgggaatac tcggaaattt gtaagtaata tttaactgct caatacatct
     4261 aacctttcag gagtcttcgg tttggccaac tcatcctcac gatattccgt tctgactgcc
     4321 gcccactggg ggcccatgct ggttgaaacc gacggcgaaa ccgtgtttag ctcgcgtggc
     4381 gcgttagcca caggaatgga aaactccttg cagagcgcgg ttcgcgacca ggttcacagc
     4441 aatacgcggg tacgatttcc aatggtgcgc aaaggctttc tcgcgtcacc ggaaaacccg
     4501 caaggcattc gtgggcagga tgaatttgtt cgcgtgagtt gggatgaggc gctggatctt
     4561 attcaccaac aacataaacg cattcgtgag gcttatggtc cggcatcgat ttttgctggt
     4621 tcctacggct ggcgttcaaa cggcgtgctg cataaggcct cgacattatt acaacgctat
     4681 atggcgctgg caggcggtta taccgggcat ctgggggatt attcgaccgg tgcggcacag
     4741 gcgatcatgc cgtatgtcgt aggtggcagc gaagtttatc aacagcaaac cagttggccg
     4801 ctggtgctgg aacatagcga tgtcgtggtg ttgtggagtg ctaacccact caatacgctg
     4861 aaaattgcgt ggaatgcatc cgatgagcag gggctttctt acttttccgc actgcgtgac
     4921 agtgggaaaa agctgatctg cattgatcca atgcgatcgg aaaccgtcga tttctttggc
     4981 gataaaatgg agtgggtggc accgcacatg ggcaccgatg ttgcgctgat gctggggatc
     5041 gcccatacgc tggtggaaaa tggttggcac gacgaagcgt ttctggcgcg ttgcaccaca
     5101 ggttatgccg tcttcgcctc ttatttgctg ggcgagagtg acggaatagc gaaaaacgcc
     5161 gaatgggcgg cagagatttg tggtgttggc gcagcgaaaa tccgcgagct ggcggctatt
     5221 ttccaccaaa ataccaccat gctgatggca ggctggggaa tgcagcgcca acagtttggt
     5281 gagcaaaaac actggatgat cgtcacgctg gcagcaatgt tggggcaaat cggcacaccc
     5341 ggcggcggtt ttggtctttc ttaccatttt gccaatggtg gtaaccccac gcggcgttct
     5401 gcggtgctct cttccatgca gggcagcttg ccgggtggct gcgatgcggt ggataaaatc
     5461 cctgttgccc gcattgttga agcactggaa aaccctggtg gcgcatatca acacaacggt
     5521 atgaaccgac atttcccgga tattcgtttt atctggtggg cgggcggtgc caactttact
     5581 catcatcagg ataccaatcg tctgatccgt gcctggcaaa aaccggagct ggtggtgatc
     5641 tctgaatgct tctggacggc ggcggcgaaa cacgcggata ttgttctgcc tgcgactacc
     5701 tcttttgagc gtaatgatct caccatgacc ggtgattaca gtaatcagca tctggtgccg
     5761 atgaagcaag tggtgccgcc acgctatgaa gcgcgtaatg attttgatgt ttttgccgag
     5821 ttgagtgaac gttgggagaa gggcggctat gcgcggttta cggaaggaaa aagtcagctg
     5881 caatggctgg aaacgtttta taacgttgcc cggcagcgcg gggcaagcca gcaggttgaa
     5941 ttgccgccat ttgctgagtt ctggcaagcc aaccagttaa ttgagatgcc ggaaaacccg
     6001 gacagcgagc ggtttattcg cttcgctgat ttttgccgcg atccgctggc gcatccgtta
     6061 aaaaccgcca gcggcaagat tgaaatcttc tcacagcgta ttgccgatta cggttacccg
     6121 gattgccctg ggcatccaat gtggctggag ccggacgaat ggcagggcaa tgccgaacca
     6181 gaacagttgc aggtactttc tgcccatccg gcgcaccgcc tgcacagcca gctgaattac
     6241 agttctctgc gcgaattgta cgcggtagca aaccgtgagc ctgtcaccat tcatcctgac
     6301 gatgctcagg cgcgcggcat aaccgaaggg gatatggtgc gggtgtggaa ctcccgtggg
     6361 cagatccttg ccggagcggt cattagcgag ggaattaaac ctggcgtgat ttgcattcac
     6421 gaaggggcat ggccggatct ggatttaacc gctgacggta tttgtaaaaa cggcgcagtg
     6481 aacgtgctga ccaaagatct ccccagctcg cggctgggga atggctgtgc gggtaatacg
     6541 acgctggcat ggctggaaaa atacaacggt ccggaactga cacttacagc gtttgaacca
     6601 ccggccagct cataatccag gtgggtagtt gggtttcatc ctgccatgcg caatcgacaa
     6661 tgtgaaaacc ctgcgcacgg taaaaatcta tcgccggttg atttttttga taaacctcca
     6721 gcatcagatg gggatagcgc tgctgcacat actgcatcag cgccttacca ataccgcgtc
     6781 tgacggcctt cggtgcgaca aacattgctg ccagaaatcg gccttccata atgctgacaa
     6841 aaccgagaag cttaccgtcc tcttcccaga cccagttttg cgcgttggca agataggcat
     6901 cccgcaccag cggaatgcag tcacgccagt aattcgcttt tataaaggga tgcccccagg
     6961 ttgtactttc cagccacagt tcgaggatcg cggggagttc tgaacgttgc gcttcccgaa
     7021 tcatggttta tttcccagat agcaacagca gccaaccaca tgatcattca ccagcccaca
     7081 tgcctgcata aaggagtaac agattgtggt gccgacaaac ttaaaaccac gttttttcag
     7141 tgccttagat agggcgtcgg aggcggacgt agatgtggga atttcgctca acgttgtggc
     7201 ttgtgtcacc tgtggctgat gatttacaaa cgaccagaca aagtcgggaa acggttcgcc
     7261 gttctgttcc atttgcaggt acgcccgcgc attaccaata attgcctgaa ttttccctcg
     7321 atggcggata atcccggcgt cctgtaccag tctttcgaca tcctcttcct gcattgctgc
     7381 gaccttcacc ggatcgaact gatgaaagta ggcgcgatag ttttcgcgtt ttttgaggac
     7441 ggtgatccac gataacccag cctgctgccc ttcaaagcag atcatttcga acagtttttt
     7501 actgtcagtt tcaggcacgc cccactcatt atcatggtag gcaatataaa gcgggccctg
     7561 actcacccag ccgcaacgtt ccatactttc cctcgctatg tacccgatcg taaaaatttc
     7621 agccgctttt cttgacgcat cggtgaaaaa gtcaatattt aatacagggc tactatgccc
     7681 gatatccttc atacaatgac aataatattg ccgaattcgg ctcttccctg gagtcgtgtt
     7741 aatgatcata aaaaaaagcg gtggtcgctg gcagctaagc ctgctggcga gcgtggtaat
     7801 cagtgccttt tttctcaaca cagcttacgc ctggcaacaa gaatatatcg ttgatacgca
     7861 acccggacat tccacagagc gttacacctg ggatagtgat catcaacctg attacaacga
     7921 tattttgtcg caacgtattc aaagtagcca aagggcgctg ggactggaag tcaatctggc
     7981 ggaagaaact cctgtggatg tgaccagcag tatgagtatg ggctggaatt ttcctttata
     8041 tgaacaggtt acaaccggcc cggtcgcggc attacattac gatggcacaa ccacctcgat
     8101 gtataacgag tttggcgaca gtactaccac gctgaccgat ccgttatggc atgccagcgt
     8161 gagtagctta ggctggcgtg ttgactcccg gcttggcgat ctccgaccct gggcgcaaat
     8221 cagctataac cagcaatttg gcgagaatat ctggaaggcg caatcaggcc tgagccggat
     8281 gacggcgaca aaccagaacg gcaactggct ggatgtcacc gtaggcgctg atatgttgct
     8341 caatcaaaat attgccgcct atgctgcgct aactcaggca gaaaatacca ctaataatag
     8401 cgactatctg tatacgatgg gggttagcgc cagattttaa cgtaacagtc acaattgaaa
     8461 ccattgaata acaacagttg tggcgatagt gggtgctaac ttaccaaata ataaatttgg
     8521 tgaataattg tcgcgttatt cattcctgaa ctaaggcatt tcattccgtt ctgatggcat
     8581 ttcatgccgt ttttccccaa gcataaaatg cacttcgtta tggttgtcgg cagagatttt
     8641 tcctttttat tactgcagga atactgccat gacaccttca aattatcagc gtacccgctg
     8701 gctgacactc atcggtacta tcattaccca gtttgcgctg gggtcggttt atacccggag
     8761 cctgtttaat ggcgcgcttt ctgccaagct ggatgcgccg gtaagccagg tcgctttctc
     8821 tttcggcttg ttaagtctgg ggctggcaat ttcgtcttct gttgcgggca aattgcagga
     8881 acgttttggc gttaaacgcg tcaccgtggc ttccggcatt ttgctgggat taggcttctt
     8941 cctgaccgcg cattccaaca acctgatgat gctgtggtta agcgccggtg tgctggtcgg
     9001 tctggcggat ggcgcgggtt acctgctgac gctctctaac tgcgtgaagt ggttcccgga
     9061 gcgtaaaggt ctgatctccg cgttcgctat cggttcttat ggtctgggta gcctgggttt
     9121 caaatttatc gacacgcagc tgctcgaaac ggtgggtctg gaaaaaacct ttgtgatttg
     9181 gggagcgatt gcgctggtga tgattgtttt cggcgcaacg ttaatgaaag acgcaccaaa
     9241 acaggaagtg aaaaccagca atggtgtggt ggagaaagat tacacgctgg cagagtcgat
     9301 gcgtaaaccg cagtactgga tgttagcggt aatgttcctg actgcctgca tgagcggcct
     9361 gtacgtgatt ggggtagcga aagatatcgc ccaaagtctg gcacaccttg atgtggtttc
     9421 cgcagccaat gcagtaacgg ttatttccat cgctaacctt tcaggtcgtc tggtgctggg
     9481 cattctgtct gacaaaattg cccgtatccg tgttatcacc attggtcagg tgatttcgct
     9541 ggtgggtatg gcggccctgc tgtttgcacc attaaatgca gtgacgttct ttgcagcgat
     9601 tgcctgcgtg gcatttaact ttggcggcac tattaccgtc tttccatcac tggtcagtga
     9661 gttcttcggc ctcaataacc tggcgaaaaa ctacggtgtg atttacctcg gtttcggtat
     9721 cggtagcatt tttggttcga ttatcgcctc actgtttggc ggtttctatg tgaccttcta
     9781 cgtgattttc gccctgctga ttctgtcatt ggcgctttct acgacgattc gtcagccaga
     9841 gcagaaaatg ttgcgtgagg cgcatggctc cctttaatgc tcagccgcga gctactgggc
     9901 ttcagcttcc gtgtgcatca aacgcatcaa cgaaatgtat aaacaatgac tcgtaaaatt
     9961 tgggccagtt aactggccct ttttactgct ttttgtaacg ctaaagtctc ttttcaaact
    10021 tgcatttttg taaatttgtg cttcatgcac actctttccc cacacttttt cgctttgctg
    10081 tggtctactt attcgcgcgt gtagatttta cttatctgac tacctccgca cttttcccct
    10141 gccgggcctg aaaagccgct aagcagggtg ttatcacctg tttgtccagg gtttgtttgc
    10201 atgagataca tcaaatcgat tacacagcag aagctgagct ttttgcttgc aatctatatt
    10261 ggccttttta tgaatggcgc ggttttttat cgccgcttcg gcagctatgc gcacgatttt
    10321 accgtctgga aaggcgtttc tgctgttgtt gaactggccg ccaccgtact ggtgaccttc
    10381 tttttactac gtcttctatc gctgtttggc cgccgcagct ggcgtattct ggcgtcgctg
    10441 gtggtgctct tttccgcagg tgccagctat tacatgacct tccttaatgt ggtcattggt
    10501 tatggcatca tcgcttccgt catgaccacc gatatcgacc tgtcaaaaga agttgttggt
    10561 ctgaacttta ttctctggtt aatcgccgtt agtgcattgc ctcttatcct tatctggaat
    10621 aaccgctgtc gctacacctt gctccgacaa ctgcgaaccc cagggcagcg tattcgcagc
    10681 ctggtggtcg tcgtactggc gggtattatg gtttgggcac cgattcgttt gctggatatc
    10741 cagcagaaga aagtggagag ggcgaccggc gttgatttgc cgagttatgg cggtgtcgta
    10801 gcgaactctt atctgccatc aaactggctt tctgcgttgg ggctgtatgc ctgggcgcgg
    10861 gtcgatgaat cttccgataa taattcattg cttaatccgg cgaagaagtt tacataccag
    10921 gctccacaaa acgttgatga tacttatgtc gtgtttatca tcggtgaaac cacgcgttgg
    10981 gaccatatgg gtattttcgg ctatgagcgt aataccacgc cgaaactggc ccaggagaaa
    11041 aatctggcgg cgttccgtgg ttactcctgt gataccgcaa ccaaactctc actgcgttgc
    11101 atgtttgtac gtcagggggg cgcggaagat aatccgcagc gcacattaaa agaacagaac
    11161 attttcgcgg ttctgaagca gttaggattc agttctgacc tctacgctat gcagagcgaa
    11221 atgtggttct acagcaacac gatggcagac aacattgctt atcgtgagca gattggtgcg
    11281 gagccacgta atcgtggcaa gccggtagat gatatgttgc tggtagacga aatgcagcaa
    11341 tcgctagggc gcaacccgga tggtaagcat ctgatcattc tgcataccaa aggttcgcat
    11401 tttaactaca cccagcgtta tccgcgtagc ttcgcgcagt ggaagccgga atgtattggt
    11461 gttgatagcg gctgtaccaa agcgcagatg atcaactcct atgacaactc ggtgacctat
    11521 gtggatcact ttatctccag cgtgattgat caggttcgcg ataagaaagc gattgtgttc
    11581 tacgcagctg accacggtga gtcaattaat gaacgcgagc acctgcacgg cacgccgcgt
    11641 gaactggcac cgccggagca gttccgcgta ccgatgatgg tctggatgtc agataaatat
    11701 ctggaaaatc cggccaatgc gcaggcgttt gcgcagctga aaaaagaagc cgacatgaaa
    11761 gtgccacgcc gtcacgtaga gctgtacgac accatcatgg gttgtcttgg ctatacttca
    11821 ccggatggtg gaattaacga aaacaacaac tggtgtcaca tcccgcagac aaaagtggca
    11881 gcggctaact aagcggcctg ctgactttct cgccgatcaa aaggcatttt gctattaagg
    11941 gattgacgag ggcgtatctg cgcagtaaga tgcgccccgc attcggtgat tggcgcagcc
    12001 tggtagcgca cttcgttcgg gacgaagggg tcggaggttc gaatcctcta tcaccgacca
    12061 aattcgaaaa gcctgctcaa cgagcaggtt tttttgcatt tgtagctcat gaggatgaga
    12121 acctccgggg gcagggaggc tcgactcgac gtaggcctga taagacgcgc cagcgtcgca
    12181 tcaggcaacg gcaccgcact tattgtcgga tgcggcgcga ccgccttatt cgaccaacac
    12241 accacacatt ttcctgccaa ctttgcgccc catatctcac tatccattaa cggatttgtg
    12301 acagaaacca ttgtcttttt ttatatttgc ttaaatcttt tttgcgttac ttatggctgg
    12361 cgctggcatt ttccgctgtg cgttttagcg ttcatggtat taatcgctca gatatccacc
    12421 ttttcctcag atttttttca ttaaatgcat aaaacttaat caccaaatgt tgctaaatat
    12481 taagctgatt gttctggtta tgttggagta gcacaaattt ctctgctgca aatagctgtt
    12541 tgaagttttt ttaatctttg tttgtggatt ctcaccgttg gtgtaaatcc cggttggctg
    12601 tattgacgtt ttcacattct gttgacagat tgtaggtcac gaggggcatt ttatggagga
    12661 tccgcactgt tacactgatg ttaattagta cggcatcccc acctcataac gttgacccga
    12721 ccgggcaaaa aacaaaaaag gtcaggcagc gacaacccac tgcaaagggt taaaacaaca
    12781 aacatcacaa ttggagcaga ataatgcgta tttccttgaa aaagtcaggg atgctgaagc
    12841 ttggtctcag cctggtggct atgaccgtcg cagcaagtgt tcaggctaaa actctggttt
    12901 attgctcaga aggatctccg gaagggttta acccgcagct gtttacctcc ggcaccacct
    12961 atgacgcctc ttccgtcccg ctttataacc gcctggttga atttaaaatc ggcaccaccg
    13021 aagtgatccc gggcctcgct gaaaagtggg aagtcagcga agacggtaaa acctatacct
    13081 tccatctgcg taaaggtgtg aagtggcacg acaataaaga attcaaaccg acgcgcgaaa
    13141 tgaacgccga tgacgtggtg ttctcgttcg atcgtcagaa aaacgcgcaa aacccgtacc
    13201 ataaagtttc tggcggcagc tacgaatact tcgaaggtat gggcttgccg gagctgatca
    13261 gcgaagtgaa aaaggtggac gataacaccg ttcagtttgt gctgactcga ccggaagcgc
    13321 cgttcctcgc tgacctggca atggacttcg cctctattct gtcaaaagaa tatgctgacg
    13381 cgatgatgaa agccggtaca ccggaaaaac tggacctcaa cccaatcgga accggtccgt
    13441 tccagttaca gcagtaccaa aaagattccc gtatccgcta caaagcgttt gatggctact
    13501 ggggcaccaa accgcagatc gatacgctgg tcttctctat tacccctgat gcttccgtgc
    13561 gttacgcgaa attgcagaag aatgaatgcc aggtgatgcc gtacccgaac ccggcagaca
    13621 tcgctcgcat gaagcaggat aaatccatca acctgatgga aatgccgggg ctgaacgtcg
    13681 gctacctctc gtataacgtg cagaaaaaac cgctggatga cgtgaaagtt cgccaagcac
    13741 taacctacgc ggtgaacaaa gacgcgatca tcaaagcggt ttatcagggc gcgggtgtat
    13801 cagcgaaaaa cctgatcccg ccaaccatgt ggggctataa cgacgacgtt caggattaca
    13861 cctacgatcc tgaaaaagcg aaagccttgc tgaaagaagc gggtctggaa aaaggtttct
    13921 ccatcgacct gtgggcgatg ccggtacaac gtccgtataa cccgaacgct cgccgcatgg
    13981 cggagatgat tcaggcagac tgggcgaaag tgggcgtgca ggccaaaatc gtcacctacg
    14041 aatggggtga gtacctcaag cgtgcgaaag atggcgaaca tcagacggta atgatgggct
    14101 ggactggcga taacggggac ccggataact tcttcgccac cctgttcagc tgcgccgcct
    14161 ctgaacaagg ttctaactac tcaaaatggt gctacaaacc gtttgaagat ctgattcaac
    14221 cggcgcgtgc taccgacgac cataacaaac gcgttgaact gtacaagcaa gcgcaggtgg
    14281 tgatgcacga tcaggctccg gcactgatca tcgctcactc caccgtgttt gaaccggtac
    14341 gcaaagaagt caaaggctat gtggttgatc cattaggcaa acatcacttc gaaaacgtct
    14401 ctatcgaata attaaaagcc atacaagact gatggcaaag gcaaaactgc ctgatgtgct
    14461 ccgcttatca ggcctacgaa aattctgcaa tgtattgaat ttgcacgatt ttgtaggccg
    14521 gataaggcgt taacgccgca tccggcataa acaaagcgca ctttgtcaac aatctgtata
    14581 cccggtggcg ctgtgcctct gcgtgcagcg ccatccggca gcaatacttc attctctgtc
    14641 cgacaagggc gggaattgat ttgtgagcaa tacagacacg cagttccagg ctgcgagtca
    14701 ctatagagaa tccgggttat gttgcagttt attctccgac gtttgggact cgtcatcccc
    14761 acgtttatcg gtattaccct tctcacattt gcctttgtcc acatgatccc gggcgatccg
    14821 gtgatgatca tggcaggcga acgtgggatc tcccctgagc gtcacgtgca gctgctggct
    14881 gaactcggct tagataaacc gatgtggcag cagtatctcc attacatttg gggcgtaatg
    14941 cacggcgatc taggcatttc aatgaaaagc cgaattccag tatgggaaga gttcgtgccg
    15001 cgctttcagg ccacgctgga acttggcgtc tgcgcgatga tttttgctac ggcagtcggt
    15061 attccggttg gtgtgctggc tgcggttaaa cgcggttcca ttttcgatca cactgcagtt
    15121 ggcctggcgc tgaccggcta ctcgatgccg atcttctggt ggggcatgat gctgatcatg
    15181 ctggtttcgg tgcactggaa cctgacgccc gtctccggtc gcgtgagcga tatggtgttc
    15241 ctcgatgact ccaatccgtt aaccggtttt atgctaatcg acaccgccat ctggggtgaa
    15301 gacggcaact ttatcgatgc cgtcgcccat atgatcttgc ccgccattgt gctgggtact
    15361 attccgctag cggtcattgt gcgtatgaca cgctcctcga tgctggaagt gctgggcgag
    15421 gattacatcc gcaccgcgcg cgccaaaggg ctaacccgca tgcgggtgat tatcgtccat
    15481 gcgctgcgta acgcgatgct gccggtagtg accgttatcg gcctccaggt gggaacattg
    15541 ctggcggggg cgattctgac cgaaaccatc ttctcgtggc ccggtctggg gcgctggttg
    15601 attgacgcac tgcaacgccg cgactatccg gtagtgcagg gcggcgtatt gctggtggcg
    15661 acgatgatta tcctcgtcaa cttgctggtc gatctgctgt acggcgtggt gaacccgcgt
    15721 attcgtcata agaagtaagg ggacatcatg tcacaggtta ctgaaaataa agtgattagc
    15781 gcaccggtgc cgatgacccc gttacaggag ttctggcact attttaaacg caacaaaggc
    15841 gcggtcgtcg ggctggttta cgtcgccatc gtgctgttca tcgcgatctt tgccaactgg
    15901 attgcgcctt ataacccggc ggatcagttc cgcgatgcgc tgctcgcccc gccagcctgg
    15961 caggaaggcg gcagtatggc acacttgctg ggcaccgatg acgtcggtcg tgatgtgctg
    16021 tcgcggctga tgtacggcgc gcgtttatcg ctgctggttg gctgtctggt ggtggtgttg
    16081 tcgctgatta tgggcgttat tctcggcctg atcgccggtt acttcggcgg cctggttgat
    16141 aacattatca tgcgcgtggt cgatatcatg ctggcgctgc caagtctgct gttggcactg
    16201 gtgctggtgg cgattttcgg cccgtcgatt ggtaacgccg cgctggcgct gaccttcgtt
    16261 gccttgccgc actatgtgcg cttaacccgc gccgccgtgc tggtggaggt gaatcgcgat
    16321 tacgtcaccg cctctcgcgt ggcaggtgcc ggggcgatgc gccagatgtt tattaacatc
    16381 ttcccgaact gccttgcgcc gctgattgtt caggcgtcgc tcggtttctc taacgccatt
    16441 ctcgatatgg ctgctcttgg tttcctcggc atgggggcac agccgccaac gcctgagtgg
    16501 ggcaccatgc tctccgacgt gttgcagttc gcgcaaagcg cctggtgggt cgtgaccttc
    16561 cctggtctgg cgatcctgtt gacggtgctg gcatttaacc tgatgggtga cggtctgcgt
    16621 gacgcgctcg atcccaaact aaagcagtaa gaggttcgag atggcgttat taaatgtaga
    16681 taaattatcg gtgcatttcg gcgacgaaag cgcaccgttc cgcgccgtag accgcatcag
    16741 ctacagcgta aaacagggcg aagtggtcgg gattgtgggt gagtccggct ccggtaagtc
    16801 ggtcagttca ctggcgatta tggggctgat tgattatccg ggccgcgtaa tggcagaaaa
    16861 actggagttt aacggccagg atttgcagcg tatctcagaa aaagagcgcc gcaacctggt
    16921 gggtgccgaa gtggcgatga tcttccagga cccgatgacc agccttaacc cgtgctacac
    16981 cgtgggtttc cagattatgg aagcgattaa agtgcatcag ggcggcaaca aaagtacccg
    17041 ccgtcagcgg gcgattgacc tgctgaatca ggtcggtatt cccgatccgg cttcgcgtct
    17101 ggatgtttac ccgcatcagc tttccggcgg catgagccag cgcgtgatga tcgccatggc
    17161 gattgcctgt cggccaaaac tgctgattgc cgatgaaccg accaccgcgc tggacgtgac
    17221 cattcaggcg caaatcatcg aactactgct ggagctacag cagaaagaga acatggcgct
    17281 ggtgttaatt acccatgacc tggcgctggt ggcggaagct gcgcataaaa tcattgtgat
    17341 gtatgcaggt caggtagtag aaacgggcga tgcacacgcc atcttccatg cgccgcgtca
    17401 cccgtatact caggcattgc tgcgtgcgct gccagaattt gctcaggaca aagaacgtct
    17461 ggcgtcgttg ccaggtgttg ttcccggcaa gtatgaccgc ccgaacggct gcctgcttaa
    17521 cccgcgctgc ccctatgcca ctgacagatg tcgcgctgaa gaaccggcgc tgaatatgct
    17581 cgctgatggg cgtcagtcca aatgccatta cccacttgat gatgccggga ggcccacact
    17641 atgagtacgc aagaggccac ctcgcaacaa ccgctgttgc aggctatcga cctgaaaaaa
    17701 cattatccgg tgaagaaagg catgttcgtg ccggaacgtc tggtgaaggc gctggacggc
    17761 gtttcgttta accttgaacg tggcaaaacg ctggcagtag tgggcgaatc tggctgcggt
    17821 aaatcgaccc tcggtcggtt gctgacgatg atcgaaacgc ccaccggcgg cgagctgtat
    17881 tatcaggggc aggatctgct caagcacgat ccgcaggcgc agaagctgcg tcggcagaaa
    17941 atccagatcg tcttccagaa tccctacggt tcgctgaatc cgcgtaagaa agttgggcaa
    18001 attctcgaag agccgctgct tattaacacc agcttaagca aagaacagcg tcgggaaaaa
    18061 gccctgtcga tgatggcaaa agtcggcctg aaaaccgagc actatgaccg ctatccgcac
    18121 atgttttccg gcggacaacg tcagcgtatc gctattgccc gtggtctgat gctcgacccg
    18181 gatgtggtga ttgccgatga gccggtttct gcgctggacg tctcagtgcg tgcgcaggtg
    18241 ctgaatctga tgatggattt gcagcaggag ttggggcttt cttatgtctt tatctcccac
    18301 gacctgtcgg ttgtggagca cattgctgat gaagtgatgg tgatgtacct aggccgctgc
    18361 gtggagaaag gaacgaagga ccaaatcttc aataacccgc gccatccgta cacccaggcg
    18421 ctgctctccg cgacgccacg cctgaacccg gacgatcgtc gtgaacgcat caaactcacc
    18481 ggtgaactgc caagcccgct gaatcctccg ccgggttgcg ccttcaacgc ccgctgtcgt
    18541 cggcgcttcg gcccctgcac ccagttgcag ccgcagctaa aagactacgg aggtcaactg
    18601 gtagcttgtt ttgctgttga tcaggatgaa aatccgcagc gttaactcac aaaaccgggg
    18661 ccatgcgctc cggtttactc aatcagcttc aacagtggcg acaagcatag caaaatgccg
    18721 taaagcagaa tcagccaggc tttaaatccg cgaagttttt ccagttgtgc gactttatag
    18781 atcaggaaaa acggaatgag gcacgacaca atgccatata acgggcttcc caactgaaag
    18841 aacaccagca ctgatacacg aaacgaaacc caaatcgtca acgtaatgac gataaaagcg
    18901 cagatcgcca gagtcagcat gcgtgagtta attttcttgg tatcaataat tcggcttaac
    18961 agattgagaa taatgccttt aatagcctcg tggaaaccga gataaatgcc aaagaatgcg
    19021 gtcagtacgg caaagatatt cagcaccgta gaggtgatat gaatgatatg tccagggatc
    19081 acctgcgcgg ccagtgccag tgccgagata ttttgttcaa aggcagaaac ggcttcttcg
    19141 tggctaattg agaaggtaaa cgaaaaggca aaaaacagga tcaccgcgat gagcgtgata
    19201 taactaattc ggtgggtacg cagcgcgagc cgggttgcca gtactttatc tgcttcccgt
    19261 ttacgatagg caatattcat tgggttaagt acctgaataa ataccgcaga aaagaagcaa
    19321 aatggaatgg taagcaggac atcgcggaaa aagacggagg cttgcgggaa ggcggttata
    19381 ttggcgaaat tccagtgcgg gatcatcgca aaaccgaaca cgacaataat ccctactttg
    19441 accaccacca ttggcccaga aatcttaaac agtaatcgtt caccaccaga cgcaatcgct
    19501 atcagtacgg cgaaaatagc gactttataa agtagagatt gtgaaagatc ggcatcggtt
    19561 aaaccgaagg ttttcaggta cgaggcgctg tcgaaaacca cggagagaga gtagataaaa
    19621 atcccgtgga taatcatcaa aaagtagata accccgagga aaattcccca gttcttcccc
    19681 aggtaatgac tgataatatc ggtgtagtca ttacaggaat cgctttcaga aagggttttt
    19741 aaataaatgt cctgcactac ccaggtgaca ggataagcaa tgatcgctgc ggtaataaat
    19801 acccaaattc ccttcaagcc aatttgtact ggcatcagca cggttccggc accaatcgcc
    19861 atgcctatgc ataataaaac ccagccgaag tcgtagcgtg taaacggcaa tttctgattg
    19921 tgtttcgata gtgtgttgtg ctgcataaag tcctgtccag aaagaagtcg gcctcgcaaa
    19981 aatgaataaa cccagtttct cttgcgcaac aggtggagaa gtcgaatacg cagtatgtaa
    20041 aaaatgaggc aacagattca ctggggaggt cgcgaggagg cgcaattcta caaaattgcg
    20101 catttcacta ccagtctgga gtcagcccgg atatgaactg caactattgc aaataaagaa
    20161 attaagctgg aaggcatcgc gcaatgagga agattgccgg gtgctgacag taaaaagcaa
    20221 tcgtagaatc agcgtcaagc cggaaggatt ccggtgaggc gcaatgttgc gggggcttaa
    20281 tccctggtgg cattggttgc tggagagaga aaacccccgc acgttgcagg tatgtacctg
    20341 acaacataac gggggctaat cttgaaccta gaccactcaa gaatagccgc gaaacgttgt
    20401 cattacaaca caggcggcta tatgacgctc gcagagctgg gcatggcctt ctggcatgat
    20461 ttagcggctc cggtcattgc tggcattctt gccagtatga tcgtgaactg gctgaacaag
    20521 cggaagtagc gtgtcatgtg ggcgtcaggc tgccgtaatg gcaatttgcg cccggaccag
    20581 gccgcagggg tgaaactctg cggccttttt cgtaagaaaa atgcaacggt tgcaagcgga
    20641 taatattctg cggaatgcgc agcacagagt gaagatgaga cggattccgg aacagaaaaa
    20701 cagtcgtaga atcagcgtca agccggaagg attccggtga ggcgcaatgt tgcgggggct
    20761 taatccctgg tggcattggt tgctggagag agaaaacccc cgcacgttgc aggtatgcac
    20821 ctgacaacac cacgggggcc attcttgcat ccaaacaata caagaatagc cgcgaaacgt
    20881 tgtcattaca atacaggcgg ctatatgacg ttcgcagaac tgggcatggc cttctggcat
    20941 gatttagcgg ctccggtcat tgctggtatt cttgccagta tgatcgtgaa ctggctgaac
    21001 aagcggaagt aacgtgccat gcgggcgtca ggctgccgta atggcaattt gcgcccggac
    21061 caggccgcag gggtgaaact ctgcggcctt tttcgtaaga aaaatgcaac ggttgcaagc
    21121 ggataatatt ctgcggaatg cgcagcacag agtgaagacg tgacggattc cggaacagaa
    21181 aaacagtcgt agaatcagcg tcaagccgga agggttccgg tgaggcgcaa tgttgcgggg
    21241 gctttatccc tggtggcatt ggttgctgga gagagaaaac ccccgcacgt tgcaggtatg
    21301 cacctgacaa catcacgggg gccattcttg aacctgaaca actcaagaat agccgcgaaa
    21361 cgttgtcatt acaacacagg cggctatatg acgctcgcag aactgggcat ggccttctgg
    21421 catgatttag cggctccggt cattgctggc attcttgcca gtatgatcgt gaactggctg
    21481 aacaagcgga agtaacgtgt catgcgggcg tcaggctgcc gtaatggcaa tttgcgcccg
    21541 gaccaggccg caggggtgaa actctgcggc ctttttcgta agaaaaatgc aacgattgca
    21601 cgcggataat attctgcgga atgcgcagca cagagtgaag acgtgacgga ttccggaaca
    21661 gaaaaacagt cgtagaatca gcggcaagcc ggaaaggttc cggtgaggcg caatgttgcg
    21721 ggggctttat ccctggtggc attggttgct ggagagagaa aacccccgca cgttgcaggt
    21781 atgcacctga caacatcacg ggggctaatc ttgaacccaa acaactcaag aatagccgcg
    21841 aaacgttggc attacaacac aggcggctat atgacgctcg cagaactggg catggccttc
    21901 tggcatgatt tagcggctcc ggtcattgct ggcattcttg ccagtatgat cgtgaactgg
    21961 ctgaataagc ggaagtaacg tgtcatgcgg gcgtcaggct gccgtaatgg caatttgcgc
    22021 ccggatcagg ccgcagaggt taaactctgc ggcctttttc gtgcttactg cgggtaaggc
    22081 acccagtcgc cgccgttcag gcgaacgtac ggtttatctt ggtattgaat aactactgca
    22141 tttgagttct cggagaccgg tgctgtttgt ggcaacccac tggtgagttt tttccagtca
    22201 acattgtctt cggtgaaaat cttgccatca agaacgcgaa ccaccagatc ggagatagcc
    22261 aggaagctgc tcggttggtc aatgacaatc ggtgccccct gatgtggtgc cttcatgccg
    22321 aagaatttca ccccaacggg gacgtctgtg atagacgggc tagggatatc acgtaggcca
    22381 gatacctgca ttctgtcgcc cttcagcgcg ccgccgtgtt ccggcaccac gaccaccatc
    22441 actttacgac ccgatttctc cagttcagta aagaaggcgt ccagttcatc aaagaatttc
    22501 tgcgcccgcg ctttgtaatc cgctgttttg ctgacccccg gataatggtt gccgtcatgc
    22561 agtggaagcg tgttgtagaa cgtggcacta cggctgtttt tatctttttc ggtaacgtcc
    22621 agccagcggt taagcacggc ggtatcgtca taaaccggcg aaccatcaaa gcccagcaaa
    22681 ataaccggca gatttgtttg atccatcaat tcagtctgca tgccgccatt ttcgcgaact
    22741 tctttcaaaa aaccgccgaa ctggccgtta tgtcccatca tcaggtgctg ggtaaagccc
    22801 agtttcgaaa ggttatcaaa cagatagcag tcgttatttg ccggttgata cagattagtg
    22861 tgcgaagtct gcccgcagct ggcgcgcagt aaacggatcg ccgccgggcc actgtaggag
    22921 gtggcggagt taaagttctt gaaatcaata tcgaaatgcg accacagtgg atgcgacatc
    22981 aacccggcgg cttctatatc cgaccaggaa agcgaacaga tgttaatcac cagtagttca
    23041 aatggctgag catcagcggg cagcgaagac gggaaggtcg atttacgttt cgcctccgcg
    23101 ttatagaaat tattcagcca ggcgttaagg ttcgccgttg ttggcggtgc agtttgtgcg
    23161 ggcatatcac ccactaccgg tgcgccaccc gtcgccgcaa cggttgccgc tgcgttacca
    23221 cccgtcgttg ttacagtggt cgtcggttgt ccggctggcc acaaggagaa acttggtccc
    23281 gccagggtaa gtacgttcag ccatagcagt atggcaacca caaaaacggt aatgcgaatc
    23341 cattgtgaca ggaataacca ggccactaat aaaacaaaaa tggccccaat catctgccag
    23401 ttaataaagc gtgtgacaag gtcgattaaa taatcggtac tgaaccccgc cacctgcgaa
    23461 ccctggctca ttatgctttc cgggccaggc aaccagatgt catgccagaa caaagcaaag
    23521 ccgatcggca gggcaatcca gtggcgcaag cgatgcaggc tgtaacgcgg aatgggcatc
    23581 agcagaaacg cggcaaacac caaattgagg agcgggtgga agttaagata tcccgcccac
    23641 aacaggccaa acttaaccag aaaatagaag ttccagccgg aaaggccgcg ccagtattgc
    23701 cagagggaag aaggcatggc ggtattttgc gtaaattgag tcattttttg gttgccctgg
    23761 ctttttccgt gcggcgtaac gtcccggccg gtttaacata acgaggttta gcaaagaaca
    23821 aacgtaaggt gtcgcgaatg cgtcgcaaag agtgccgcgc cagatagccc agcgggaaaa
    23881 atatcagtgc gcaaacgata ataatttcaa tgatatcgct gatggtcatc atgatgagcg
    23941 ctccacagca tcatctaaca gtcgcatggg ttctggtatt cgtcgccagt ggcgaccatc
    24001 gtgctcggca ttgatgaccg gtttagaact ttgcgttaaa ggcagcggca ttccccattg
    24061 ttctggggca agcaggcgca tctgcaccag ctcggcactg atttgatcat cttcaaacca
    24121 gaccatacgg tttgagaaaa tgtcgccagt aggcaatggg aaaatatgat tcaacgcggt
    24181 atccagatcg ttaatccgac agaatgagag aaacagcacc agccgattac cgccaatggt
    24241 catgatatcg ccggtacggt tagggcgaca tagcgtcagg gcttgctcaa cgcggatacc
    24301 cggtaccgga cgtagggcaa ccagaacgcc tttaccgtgg gcaggcaaga gagggttatt
    24361 catcatgttg ttgacggcat tacagaacac atcccacttc tggaaaccac gcagtttgag
    24421 cggctgggtc attgacagca aggtagtgat atcttccggt acatagcgac taaacttctg
    24481 cccttgcacg ctttcgatca tcgtcagaca acgggagagt ggcgcattcc acggaataac
    24541 catatttgca ccgcaggcca ataacaaacg ttcatcggtg gcgcgcaggc tagcggtatt
    24601 ttcccgcacg aggattttca tcgcactacc gcgctggcga cgcagggtat gaatgctgcg
    24661 ggccagtggc tcgatttgcg cattttgctg taaagaaaag accaccgtcg ccgcctgagc
    24721 ggtacgggct tcattgaaca ggacttcgtt attgttgaac agttgccagt gttccgatag
    24781 cggcggcgca ccttccagta cagcaacatt actcagaatg cgtttttcgt cgctgcgtgg
    24841 ttggatctcc gcctcttcgc tttgaactaa tgtccagata ccattttgtt gctgaacgct
    24901 aagctgctga cgggcgctga cccctttttc gttgcaccag aaggcaatat ccagcaaatg
    24961 ttggtcaccc tgaaaacgca aactggcaag accaaaaagt gaacggtatt cctcaagcaa
    25021 caatgaaaat tgtttatcgt tattatttcc gggattaatt accaaaagcg aacaatggtt
    25081 taacctgctc catttattca ttttatccaa ccatgagcga agccgctcgg caggaatatt
    25141 ttgccatgcg ttatttgcgc aaacaagaat aaaaaggtaa ttatggggat caatagaaca
    25201 ctgcaaatca cgggccaaat agtatagacc tttttcatga tttagcattg aaaataattt
    25261 tattttttcc ggcccttgag aatcatctaa ttgaaagatt ttcgccggat cgctgtccat
    25321 gctaatgacc gcgacgtgtg cggtttcagc ctgggatgca attgtttgat tcgccagact
    25381 gatagcatct tcatggcgat cgacgttaaa ccaccagacg ccgcctgctg gcatatgtcg
    25441 cagctcatcc cataatgatg agataccgat agagaataca gggtccacaa tgtccctcat
    25501 gtcaccattt tgtctgatta tcagtttact agcgaaagct aagaaataaa cctaccattg
    25561 aaattaaaac ttatcagatt tagcatgtga atcaatttag aaggtcacaa gacgtgtgat
    25621 tttagtcttt tcgtttttaa ctttttctgt tgcgtaacgg aaagtcaaaa agtgagcaaa
    25681 ttcccgtctc gccgtaaaca ataaaccggc gaaatgctca cggttagaat tgtctcatga
    25741 atggtacggt tatttcatag ggatcaagca aaatgaataa caatgaacca gatactctgc
    25801 ctgatcccgc gataggctat atcttccaga atgatattgt ggcgttaaag caggcatttt
    25861 cactgcctga tattgattat gccgatattt cccaacgcga acagttggcc gcggcattaa
    25921 aacgctggcc gttgctggca gagtttgcgc aacaaaagta ggggattggt gaatggccgt
    25981 actgggattg cagggggtgc ggggaggcgt ggggacaaca accatcaccg ccgcattagc
    26041 ctggtcatta caaatgttgg gagaaaatgt cctggtggtc gatgcctgcc cggacaactt
    26101 gttgcgcctg tcatttaatg ttgattttac ccaccgtcag ggctgggcca gagcgatgct
    26161 ggatggccag gactggcgtg acgctgggtt gcgctacacc tcgcagctcg atttgctgcc
    26221 ttttggtcag ttatccattg aagaacaaga aaatccacag cactggcaaa cccggctgag
    26281 cgatatttgc tccggcttac agcaactaaa agccagcggg cgttaccagt ggattttaat
    26341 cgacttaccg cgtgatgcct cgcagataac ccaccagctg ctgagtttgt gcgagcactc
    26401 gctggcaatc gtcaatgtgg atgccaactg ccatatccga ctgcatcagc aagcgctgcc
    26461 ggatggcgca catattttga ttaatgactt ccgtattggc agtcaggttc aggacgatat
    26521 ttaccagctt tggttgcaaa gccagcgccg attactgccg atgctcattc atcgtgatga
    26581 agcgatggct gaatgcctgg cggctaagca accagtaggt gaatatcgca gtgatgcgct
    26641 ggcggctgaa gagatactga cgctggcgaa ctggtgcctg ttgaactact ccgggctgaa
    26701 aacgccagtc gggagtgcat catgagtatc ctgacccggt ggttgcttat cccgccggtc
    26761 aacgcgcggc ttatcgggcg ttatcgcgat tatcgtcgtc acggtgcgtc ggctttcagc
    26821 gcgacgctcg gctgtttctg gatgatcctg gcctggattt ttattccgct ggagcacccg
    26881 cgctggcagc gtattcgcgc agaacataaa aacctgtata cgcatatcaa cgcctcgcgt
    26941 ccgcgtccgc tggacccggt ccgttatctc attcaaacat gctggttatt gatcggtgca
    27001 tcgcgcaaag aaacgccgaa accgcgcagc gggcattttc aggtctgcaa aatattcgtg
    27061 gacgttacca tcaatggatg aacgagctgc ctgagcgcgt tagccataaa acacagcatc
    27121 tggatgaaaa aaaagagctc ggtcatttga gtgccggggc gcggcggttg atcctcggta
    27181 tcatcgtcac cttctcgctg attctggcgt taatctgcgt tactcagccg tttaacccgc
    27241 tggcgcagtt tatcttcctg atgctgctgt ggggggtagc gctgatcgta cggcggatgc
    27301 cggggcgctt ctcggcgcta atgttgattg tgctgtcgct gaccgtttct tgccgttata
    27361 tctggtggcg ttacacctct acgctgaact gggacgatcc ggtcagcctg gtgtgcgggc
    27421 ttattctgct cttcgctgaa acgtacgcgt ggattgtgct ggtgctcggc tacttccagg
    27481 tagtatggcc gctgaatcgt cagccggtgc cattgccgaa ggatatgtcg ctgtggccgt
    27541 cagtggatat ctttgtcccg acttacaacg aagatctcaa tgtggtgaaa aataccattt
    27601 acgcctcgct gggtatcgac tggccgaaag acaaactgaa catctggatc ctcgatgacg
    27661 gcggcaggga agagtttcgc cagtttgcgc aaaacgtggg ggtgaaatat atcgcccgca
    27721 ccactcatga acatgcgaaa gcgggcaaca tcaacaatgc gctgaaatat gccaaaggcg
    27781 agttcgtgtc gattttcgac tgcgaccacg taccaacgcg atcgttcctg caaatgacca
    27841 tgggctggtt cctgaaagag aaacagctgg cgatgatgca gactccacac cacttcttct
    27901 cgccggaccc gtttgaacgc aacctgggac gttttcgtaa aacgccgaac gaaggcacgc
    27961 tgttctatgg tctggtgcag gatggcaacg atatgtggga cgccactttc ttctgcggtt
    28021 cctgtgcggt gattcgtcgt aagccgctgg atgaaattgg cggcattgct gtcgaaaccg
    28081 tgactgaaga tgcgcatact tctctgcgtt tgcaccgtcg tggctatacc tccgcgtata
    28141 tgcgtattcc gcaggcggcg gggctggcga ccgaaagtct gtcggcgcat atcggtcagc
    28201 gtattcgctg ggcgcgcggg atggtataaa tcttccgtct cgataacccg ctcaccggta
    28261 aaggactgaa gtttgctcag cggctgtgct acgtcaacgc catgttccac ttcttgtcgg
    28321 gcattccacg gctgatcttc ctgactgcgc cgctggcgtt cctgctgctt catgcctaca
    28381 tcatctatgc gccagcgttg atgatcgccc tattcgtgct gccgcatatg atccatgcca
    28441 gcctgaccaa ctcgaagatc cagggcaaat atcgccactc tttctggagt gaaatctacg
    28501 aaacggtgct ggcgtggtat atcgcaccac cgacgctggt ggcgctgatt aacccgcaca
    28561 aaggcaaatt taacgtcacc gccaaaggtg gactggtgga agaagagtac gtcgactggg
    28621 tgatctcgcg gccctacatc ttccttgttc tactcaacct ggtgggcgtt gcggtaggca
    28681 tctggcgcta cttctatggc ccgccaaccg agatgctcac cgtggtcgtc agtatggtgt
    28741 gggtgttcta caacctgatt gttcttggcg gcgcagttgc ggtatcggta gaaagcaaac
    28801 aggtacgccg atcgaaccgc gtggagatga cgatgcccgc ggcaattgcc cgcgaagatg
    28861 gtcacctctt ctcgtgtacc gttcaggatt tctccgacgg tggtttgggg atcaagatca
    28921 acggtcaggc gcagattctg gaagggcaga aagtgaatct gttgcttaaa cgcggtcagc
    28981 aggaatacgt cttcccgacc caggtggcgc gcgtgatggg taatgaagtt gggctgaaat
    29041 taatgccgct caccacccag caacatatcg attttgtgca gtgtacgttt gcccgtgcgg
    29101 atacatgggc gctctggcag gacagctacc cggaagataa gccgctggaa agtctgctgg
    29161 atattctgaa gctcggcttc cgtggctacc gccatctggc ggagtttgcg ccttcttcgg
    29221 tgaagggcat attccgtgtg ctgacttctc tggtttcctg ggttgtatcg tttattccgc
    29281 gccgcccgga gcggagcgaa acggcacaac catcggatca ggctttggct caacaatgat
    29341 gataacgcga tgaaaagaaa actattctgg atttgtgcag tggctatggg gatgagtgcg
    29401 ttcccctctt tcatgacgca ggcgacgcca gcaacgcaac cactgatcaa tgctgagcca
    29461 gctgtagccg cccagacgga acaaaatccg caggtggggc aagtgatgcc aggcgagcag
    29521 ggagctgatg cccccatcgt ggcgcagaac ggtccttcgc gtgatgtgaa gctgaccttt
    29581 gcgcaaatcg cgccgccgcc gggcagcatg gtgctacgtg gtattaaccc gaacggcagc
    29641 attgagtttg gtatgcgcag cgatgaagtg gtgacgaagg cgatgctcaa cttcgaatac
    29701 acgccatcgc catcgttact gcctgtccag tcgcagttaa aggtttatct caatgatgag
    29761 ctgatgggcg tgctgccagt gaccaaagaa cagttgggta aaaaaacgct ggcgcaaatg
    29821 cccattaatc cactgtttat taccgacttc aaccgtgtgc ggctggagtt tgtcggccat
    29881 tatcaggacg tgtgcgaaaa cccggccagc accacgcttt ggctggatgt tgggcggagc
    29941 agtggactgg atctgaccta tcagaccctg aatgtgaaga atgacctgtc acacttcccg
    30001 gtgccattct ttgacccgcg cgataaccgt actaacacct tgccgatggt ctttgcgggt
    30061 gcgccggatg ttgggctgca acaagcatct gccattgtcg cctcgtggtt tggttcgcgt
    30121 tctggctggc gtgggcagaa cttcccggtg ctctataacc aattgccgga tcgcaatgcc
    30181 attgtctttg caaccaacga caaacggccg gacttcctgc gcgatcatcc ggcggtaaaa
    30241 gccccggtga ttgagatgat taaccatccg cagaatcctt acgtcaaact gctggtggtg
    30301 tttggtcgtg acgacaaaga cctgttgcag gcagcgaaag gtatcgctca gggtaacatt
    30361 ctgttccgtg gtgaaagcgt ggtagtgaat gaagtgaaac cgctgctacc gcgtaagccg
    30421 tacgatgcgc cgaactgggt acgtaccgat cgtccggtca catttggcga actgaaaacc
    30481 tatgaagaac agttacaatc cagcggcctt gagccagcag cgattaacgt ttcgctaaac
    30541 ctgccgccgg atctctacct gatgcgcagt accggcattg atatggatat taattaccgc
    30601 tacaccatgc cgccggtgaa agacagttcg cggatggata tcagcctgaa taaccagttc
    30661 ctgcaatcct tcaacctgag cagcaaacag gaggcgaacc gtctgctgct gcgtattccg
    30721 gtattacaag gtttgctgga tggcaaaaca gatgtctcta ttccggcgct gaaactgggc
    30781 gcgaccaacc agctgcgctt cgactttgag tatatgaacc cgatgccagg cggttcggta
    30841 gataactgta ttaccttcca gccggtgcag aatcatgtgg tgattggtga cgattccacc
    30901 atcgacttct cgaagtatta ccacttcatc ccgatgccgg atctacgcgc ctttgctaac
    30961 gcgggcttcc cattcagccg gatggcggat ctgtcgcaaa ccatcaccgt gatgccgaaa
    31021 gcgcctaacg aagcacagat ggaaacgttg ctgaatactg ttggttttat cggcgcacag
    31081 acgggcttcc cggcgattaa tctgacggtg accgatgatg gcagcaccat tcagggcaaa
    31141 gatgccgaca tcatgatcat cggtggtatc ccggacaaac tgaaagacga taagcagatc
    31201 gacctattgg tgcaggcgac cgaaagctgg gtgaaaacac cgatgcgcca gaccccgttc
    31261 cccggcattg tgccggacga gagcgatcgc gcggcagaaa cccagtcaac gctgacctct
    31321 tccggtgcga tggcggcggt gattggcttc cagtcgccgt ataacgacca gcgcagcgtg
    31381 attgcgctgt tggcagatag cccacgcggt tatgaaatgc ttaacgatgc ggtgaacgat
    31441 agcggcaaac gcgccaccat gttcggttcg gtcgcggtga tccgcgagtc cggtatcaac
    31501 agcctacgtg ttggcgacgt ttattacgta ggtcatctgc cgtggttcga gcgcttgtgg
    31561 tatgcgctgg caaaccatcc gattctgctg gcggtgctgg cgactatcag tgtgatattg
    31621 ctggcatggg tactgtggcg tctgctgcga attattagtc gtcgtcgtct taacccggat
    31681 aacgagtaat tgaagatgaa tgtgttgcgt agtggaatcg tgacgatgct gttgttggct
    31741 gcctttagtg ttcaggcagc ctgtacctgg cctgcctggg agcagtttaa aaaggattac
    31801 atcagtcagg aagggcgcgt catcgacccc agcgacgcgc gcaaaatcac cacctccgaa
    31861 gggcaaagtt acggtatgtt ctttgccctg gcggctaacg accgtgcagc tttcgataat
    31921 attctcgact ggacgcagaa caatctcgct cagggttctt taaaagaacg tttgcccgcc
    31981 tggctgtggg gcaagaaaga gaacagtaag tgggaagtgc tggacagcaa ttcggcctcc
    32041 gatggtgatg tctggatggc ctggtcgttg ctggaggcgg ggcgtttgtg gaaagagcag
    32101 cgttataccg acatcggcag cgcgttgcta aaacgtatcg cgcgggagga agtggtgacg
    32161 gtgcctgggc tgggttccat gttgttaccg ggcaaagtgg gttttgctga ggataacagc
    32221 tggcgtttta accccagcta catgccgccg acgctggcgc agtatttcac ccgctttggc
    32281 gcgccgtgga ccacgctgcg cgaaaccaat caacatttat tgctggaaac cgccccgaaa
    32341 ggcttttcgc cagactgggt gcgctatgag aaagacaaag gctggcagct aaaagccgaa
    32401 aaaacattga tcagcagcta cgacgctatc cgcgtttaca tgtgggtagg catgatgcct
    32461 gacagcgatc cgcaaaaagc gcggatgctc aaccggttta aaccgatggc gacattcact
    32521 gagaaaaacg gttatccgcc ggaaaaagtg gatgtggcta cggggaaagc gcagggtaaa
    32581 ggaccggtcg gtttttctgc cgccatgctg ccttttttac aaaaccgcga tgcgcaggca
    32641 gttcagcgcc agcgcgtggc cgataacttt cccggcagcg atacctatta caactatgtg
    32701 ctgaccctgt ttggacaagg ctgggatcaa caccgtttcc gcttctcgac aaaaggtgag
    32761 ttattacctg actggggcca ggaatgcgca aattcacact aaacatattc acgctttccc
    32821 tcggtctggc cgtcatgccg atggtcgagg cagcaccaac catcagcaga ccctgcggat
    32881 ggacagcggc aacactaatg ccgtgcgcgg gctggcgaat atttaccgcc agcagtcgcc
    32941 agaaaaagct gaagcgttta tcgcctcgct ctctgccagc cagcggcgca gcattgatga
    33001 tatcgaacgc agcctgcaaa acgaccgtct ggcacagcag gcagaggtac tggaaaacca
    33061 gggcaaatgg gcgcaggcgg cagcacttca gcggcaacga ctggcgctgg gccccggcag
    33121 cgtatggatt acttaccgac tggcaggaag aagctgcgcg gcaatccaac gatggcggca
    33181 gcagtcaggg ctttggctac acggcgcggg cattacttga acgacgtgtt acttccaact
    33241 ggtttgttgg cacggcaatt gatatccagc aggtgaaaga ttacgcaccc agccatttcc
    33301 tgctctacgt acgttattcc gccgccggat ggcagggtga catggattta ccgccgcagc
    33361 cgctgatacc ttacgccgac tggtaagttt tcagatagcg cctctcttaa tgccgctgcg
    33421 atcgggtata ctcgggcggc aatctgggat ttccgggggg agacaatttg cgcgtaagtc
    33481 gctcgttaac aatcaagcag atggcaatgg tggcagccgt tgtcctggtg ttcgttttta
    33541 ttttttgcac cgttttgctg ttccatctgg tccagcagaa tcgctataac acggctacgc
    33601 aactggaaag cattgctcgc tctgtccgcg aacccttatc ttcagctatt ttgaaaggcg
    33661 atattcccga agcggaagct attcttgcca gcattaaacc ggcaggcgtg gtcagccgtg
    33721 ccgatgtagt gctgcctaac cagttccagg cgctgcgtaa aagttttatt ccagagcgtc
    33781 cggtgccggt aatggttact cgcctgtttg agctaccggt tcaaatctcg ctgggcgtct
    33841 actcgctcga acgtccggca aatccgcagc caatagccta tctggtgcta caggcggatt
    33901 ccttccgtat gtataagttc gtgatgagca ctctctcaac gttagtgacc atttacttac
    33961 ttttgtcgct tatcctgacc gtcgccatca gctggtgcat taaccgcctg attttgcatc
    34021 cgttacgcaa tattgctcgc gaacttaacg ccatcccagc ccaggagctt gttggtcacc
    34081 aactggcatt accgcgtctg catcaggacg atgaaatcgg tatgttggtg cgcagttaca
    34141 acctcaacca gcaattgctg cagcgccatt atgaagaaca gaacgaaaat gcgatgcgct
    34201 tcccggtgtc ggatttgccg aacaaagcct tgctgatgga gatgctggag caggttgtcg
    34261 cgcgtaaaca aaccaccgcg ctgatgatca tcacctgtga aaccctgcgt gatactgcgg
    34321 gcgtgctgaa agaggcgcaa cgagaaattc tgctgctgac gctggtggaa aaactcaaat
    34381 cggtactgtc gccacgtatg atcctcgcgc agattagcgg ttatgacttt gctgtcattg
    34441 ccaacggtgt acaggaaccg tggcacgcaa tcactttggg tcagcaagta ctcactatca
    34501 tgagcgagcg cctgccgatt gaacgtattc aactccgtcc gcactgtagc attggcgtgg
    34561 cgatgttcta cggcgatctc accgccgaac agctttacag tcgcgctatt tctgcggcat
    34621 ttaccgctcg ccataaaggc aagaatcaga ttcagttctt tgatccgcag cagatggaag
    34681 cagcccagaa gcggttgacg gaagagagcg atatccttaa tgcactggaa aatcatcagt
    34741 ttgcaatttg gttacagcca caggtcgaga tgaccagcgg taaactggtc agtgcggaag
    34801 tgttactgcg tatccagcaa ccggatggca gttgggacct gccggatggc ttaatcgatc
    34861 gcattgagtg ctgtgggctg atggttaccg tcggtcactg ggtgctggaa gagtcctgtc
    34921 gattgcttgc agcctggcaa gagcgcggca ttatgctgcc cttgtcggta aacctctctg
    34981 cgctgcaact gatgcacccg aatatggtgg cggatatgct ggaactgtta acccgctatc
    35041 gcattcagcc gggaacactg attctggaag tgacagaaag ccgacgtatt gacgaccctc
    35101 atgctgcggt ggcaatcctc cgtccgctgc gcaatgccgg agttcgggtg gcgctggatg
    35161 atttcggcat gggctacgca gggctgcgtc agctgcagca tatgaaatcg ttgccaatcg
    35221 acgtactgaa aatcgacaaa atgtttgttg aaggcttgcc ggaagatagc agcatgattg
    35281 ctgcaattat catgctggcg cagagcctga acttacaaat gattgccgaa ggcgtggaga
    35341 ctgaagcaca acgagactgg ctggcaaaag cgggcgttgg tattgcccag ggcttccttt
    35401 ttgctcgccc actccctatt gaaatcttcg aagagagtta cctggaagaa aagtagctac
    35461 cccaaactga ttacaaaact ttaaaaagtg ctggtttgtg cgagccagct caaacttttt
    35521 aacctttttg tttcaattat gatccaggta catttctgtg atgttgtctg ggtgttattt
    35581 taaggccgca ggtacccccc ccataacctt acaagacctg tggttttact aaaggacacc
    35641 ctatgaaaac ctctctgttt aaaagccttt actttcaggt cctgacagcg atagccattg
    35701 gtattctcct tggccatttc tatcctgaaa taggcgagca aatgaaaccg cttggcgacg
    35761 gcttcgttaa gctcattaag atgatcatcg ctcctgtcat cttttgtacc gtcgtaacgg
    35821 gcattgcggg catggaaagc atgaaggcgg tcggtcgtac cggcgcagtc gcactgcttt
    35881 actttgaaat tgtcagtacc atcgcgctga ttattggtct tatcatcgtt aacgtcgtgc
    35941 agcctggtgc cggaatgaac gtcgatccgg caacgcttga tgcgaaagcg gtagcggttt
    36001 acgccgatca ggcgaaagac cagggcattg tcgccttcat tatggatgtc atcccggcga
    36061 gcgtcattgg cgcatttgcc agcggtaaca ttctgcaggt gctgctgttt gccgtactgt
    36121 ttggttttgc gctccaccgt ctgggcagca aaggccaact gatttttaac gtgatcgaaa
    36181 gtttctcgca ggtcatcttc ggcatcatca atatgatcat gcgtctggca cctattggtg
    36241 cgctcggggc aatggcgttt accatcggta aatacggcgt cggcacactg gtgcaactgg
    36301 ggcagctgat tatctgtttc tacattacct gtatcctgtt tgtggtgctg gtattgggtt
    36361 caatcgctaa agcgactggt ttcagtatct tcaaatttat ccgctacatc cgtgaagaac
    36421 tgctgattgt actggggact tcatcttccg agtcggcgct gccgcgtatg ctcgacaaga
    36481 tggagaaact cggctgccgt aaatcggtgg tggggctggt catcccgaca ggctactcgt
    36541 ttaaccttga tggcacatcg atatacctga caatggcggc ggtgtttatc gcccaggcca
    36601 ctaacagtca gatggatatc gtccaccaaa tcacgctgtt aatcgtgttg ctgctttctt
    36661 ctaaaggggc ggcaggggta acgggtagtg gctttatcgt gctggcggcg acgctctctg
    36721 cggtgggcca tttgccggta gcgggtctgg cgctgatcct cggtatcgac cgctttatgt
    36781 cagaagctcg tgcgctaact aacctggtcg gtaacggcgt agcgaccatt gtcgttgcta
    36841 agtgggtgaa agaactggac cacaaaaaac tggacgatgt gctgaataat cgtgcgccgg
    36901 atggcaaaac gcacgaatta tcctcttaat ctcaccactt atgcccgcac tcccttcccc
    36961 gagtgcgggt gtttgcgcat aattgacccc tgtgcctgaa cttaccacct gggtgagttc
    37021 aggtttattt cacttcttcc gtgacatttt catgttcttg cggtctaaca cgaagtgttt
    37081 ttaacgtcat ttagactgac cgacaacggg cagttttttt attaccagga tagttgatca
    37141 ggggttcaca tgcagggcat aaaaattcga cttttagcgg gcggtttgct gatgatggcc
    37201 actgctggct atgtgcaggc agatgcgctc cagcctgatc cagcatggca acaggggacg
    37261 ctttccaacg gtttacagtg gcaagtgctg accacccccc agcgtcccag cgatcgtgtt
    37321 gaaattcgcc tgctggttaa taccggttcg ctcgccgaaa gtacacaaca gagcggttac
    37381 agtcacgcca tccctcgtat tgcgctaacg caaagcggtg gccttgacgc agcacaggcg
    37441 cgttcattgt ggcagcaggg gatcgaccct aaacgcccga tgccgccggt aattgtctct
    37501 tatgacacca cgctgtttaa tctgagtttg cccaataacc gtaacgattt gctgaaagaa
    37561 gcgctctctt atctggcaaa tgccactggc aaattgacca tcacaccaga aaccatcaac
    37621 cacgcgctgc aaagtcagga catggtggca acctggcctg ccgatactaa agagggctgg
    37681 tggcgctatc gtctgaaagg gtcaaccttg ttaggtcacg atcctgccga tccgctgaaa
    37741 caacccgttg aagcggaaaa aattaaagat ttctatcaga aatggtacac cccggatgca
    37801 atgacgctac tggtggtggg aaacgtggat gcgcgctcgg ttgtcgacca aatcaacaaa
    37861 acgtttggcg aactgaaagg caaacgtgaa acgccagctc cggtgccgac gctttctccg
    37921 ctgcgtgcgg aagcggtgag tattatgact gacgcggtgc gtcaggaccg gttatctatc
    37981 atgtgggata cgccgtggca gccgattcgt gaatcagccg cactgctgcg ctactggcgt
    38041 gcggacctgg cccgcgaggc gctgttctgg catgttcagc aagcgttaag cgccagtgac
    38101 agcaaagaca tcggtcttgg atttgactgc cgtgtgctgt atctgcgtgc gcagtgtgcc
    38161 atcaacatcg aatcaccaaa cgacaagctg aacagcaacc ttaatctggt ggcgcgtgaa
    38221 ctggcgaagg ttcgcgataa aggtctgccg gaagaagagt tcaatgcgtt agtggcgcaa
    38281 aagaaactgg agctgcagaa actgtttgcc gcctatgcac gggctgatac cgatattctg
    38341 atgggccagc ggatgcgttc gttgcaaaat caggtcgtcg atatcgcgcc ggagcagtat
    38401 cagaaactgc ggcaggattt ccttaacagc ctgactgtgg agatgttaaa tcaggatctg
    38461 cgtcagcagt tgtcgaatga tatggcgtta atactgctgc agccgaaagg cgagccggaa
    38521 tttaacatga aagcgttgca ggcggcctgg gatcaaatca tggccccatc taccgccgct
    38581 gcgaccacct ctgtcgccac ggatgacgta catcctgaag tgacggatat tccacctgca
    38641 cagtaatgcc tgatggtgat gagttagcac aaccatcagg tatttatgga tgagctggat
    38701 agaggggtaa cactttatcc agccttttgc atatgctgcg tttacgctgg catcgcctca
    38761 cgcgggataa tcgcgccgcg atactgaata acggtacttg cggtcaggtg cccgcgtttc
    38821 gccgcgtttt ccgcactgcc gcccgtcaaa cgtaccgcca gataaccggc gctgaaagag
    38881 tcgccagctg cggtggtatc gatcactttt tctttcggca gtttcaccgc cggaacatca
    38941 actaacgctt caccagcaat ggacaccagg caagaatccg ccccgcgttt caccaccact
    39001 tctttcacgc ccgcgttatg ggtgcgcgca atgacgtctt ccaccggctg ttgaccccac
    39061 agcgcgtctt cgtcgtccag cgtcaggaag gcgatatccg tgcattccag catttgctgg
    39121 tacacctgct gtgtctcttc tttgctggcc cacaggcgcg gacgatagtt attgtcgaaa
    39181 atcacttttc cgcctttggc gcggcattcg cgcagcaggg aaagcagctt ttcgcggctg
    39241 gtcgggctta agatcgccag gctaatcccg ctcaggtaga gataatcgaa attcgccagc
    39301 tcttcgcaaa tcgccgcaga ctgctcgctc gccagccaga atttggcggc ggcttcgtta
    39361 cgccagtagt agaacgtacg ttcgccggtg ctgtcggttt caatgtagta aaggcccgga
    39421 agacggtttt ccatccgttg ggtcagggaa gtatcaacgt tctcgccgtg ccaggcgtcc
    39481 agcatctgct ggctaaaact gtccgttccc agcgccgtta cgtaatgaac ggttaatgcc
    39541 gcaggatcga cctgacgggc gatatagacg gaagtgttca gggtatcgcc gccgaaaccg
    39601 cgcttaacgt ccgcgccttt ctcggaaagc tcaatcatgc attcgccaat cacggcaatc
    39661 tttttggaca tagtcgtgaa cctgatctgt aaaaaattag cgttagtttg cgctgtggtg
    39721 gtttgctggt caaccatatt aaaacagtgt tccattattt ttttgagcca gaacgtattc
    39781 ctgaaagatt ccgttgtgga gtgaggaaat ttaccccggc caggcggtaa agttctgccc
    39841 ttacgcgccg ataatctttg tcgagtccgg gcagcatcac ttttaaacac aggacatctt
    39901 tgatgataag gcaggttatc cagcgaataa gcaaccctga agcaagcatc gagagcttgc
    39961 aggaacggcg tttttggttg cagtgtgagc gtgcttacac ctggcagccg atctatcaaa
    40021 catgcgggcg gttaatggcc gtggagctat taacggtggt cacgcatccc ttgaaccctt
    40081 cgcaacgcct gccgccggat cgctatttta ctgaaatcac cgtcagccat cggatggagg
    40141 ttgtgaaaga gcagattgat ttgctggcgc aaaaagccga cttctttgta gagcacggcc
    40201 tgctggcatc ggtcaatatt gatggcccta cgctcatcgc cctgcgtcag caaccaaaaa
    40261 tcctgcgcca gattgagcgt cttccctggc tgcgtttcga actggtggag catatccgtc
    40321 tgccgaaaga ttcaaccttt gcctcgatgt gtgaatttgg cccgctgtgg ctggatgatt
    40381 ttggtaccgg gatggcaaat ttctctgcac taagtgaagt gcgttatgac tacatcaaaa
    40441 tcgcgcgaga actgtttgtg atgctgcgtc agtcgccgga aggccgcaca ctcttttctc
    40501 agcttttaca tctaatgaat cgctattgtc gcggggtgat tgtcgagggc gtagaaacac
    40561 cggaagagtg gcgtgatgtt cagaactcgc ccgcattcgc cgcacaaggc tggtttcttt
    40621 cacgcccggc accgatggaa acgctgaata cggcggttct ggcgctataa gctgcctcat
    40681 tttccgccgg gctggactat ctttaggact ggcacaggaa gaatatgagg aagcgaacga
    40741 tgagcaaggc aggcaaaata accgctgcga tttcaggggc tttcttgttg ttgattgtcg
    40801 tggcgatcat tttgattgca acttttgact ggaatcgact caaaccgacc atcaaccaga
    40861 aagtctctgc ggagttgaat cgtccgttcg ctatccgtgg caatctgggc gtggtgtggg
    40921 agcggcaaaa acaagaaact ggctggcgca gctgggtgcc gtggcctcat gtacatgcag
    40981 aagacatcat tcttggtaat ccaccggata ttcccgaagt cacgatggtg catttgccgc
    41041 gagtagaggc aacgctggca ccgctggcgc tgctgaccaa aacggtctgg ctgccgtgga
    41101 tcaagctcga aaagcccgac gcgcgcctga ttcgcctctc tgaaaagaac aataactgga
    41161 cgtttaatct tgccaacgat gacaacaaag acgcgaatgc aaagccgtcg gcatggtcgt
    41221 ttcggctgga taatattctt ttcgatcaag ggcggatcgc cattgatgac aaagtaagca
    41281 aagcggatct ggaaattttt gttgatccgt taggcaagcc gctgccattc agcgaagtta
    41341 ctggatcgaa aggtaaagcg gataaagaaa aggtgggcga ttacattttt ggcctgaagg
    41401 cgcagggacg ttataacggt gaaccgctca cgggtacggg aaaaataggc ggtatgctgg
    41461 cgctgcgtgg cgaagggacg ccgtttccgg tacaggctga tttccgttca ggtaataccc
    41521 gtgttgcttt tgatggcgtc gtgaatgacc caatgaagat gggcggtgtc gatttacggc
    41581 ttaaattttc tggcgattca ctgggtgatc tctatgaact gacgggcgtt ctgctgcccg
    41641 ataccccgcc gtttgaaacg gatggtcggc tggtagcgaa aatcgacact gaaaaatcgt
    41701 cggtctttga ttatcgcggt tttaatgggc gaattggcga tagcgatatc cacggttctc
    41761 tggtctacac caccggaaag ccacgaccaa aactggaagg tgatgtcgag tcgcggcaat
    41821 tgcggctggc ggacctggga ccgttgattg gcgttgattc cgggaaaggg gcagaaaagt
    41881 cgaaacggtc tgaacagaag aagggcgaaa aaagcgttca gcctgcgggc aaagtgctgc
    41941 cttatgaccg cttcgaaacc gataaatggg acgttatgga tgccgatgtt cgcttcaaag
    42001 ggcggcgcat tgagcatggc agtagcctgc cgattagcga tctttctact catatcatcc
    42061 tcaaaaatgc tgacctgcgc ctgcaaccgc tgaaatttgg catggcgggc ggcagcattg
    42121 cggcgaatat tcatctggaa ggcgataaaa agccgatgca ggggcgggca gatattcagg
    42181 ctcgtcgact gaaactgaaa gaactgatgc ccgatgtgga actgatgcag aagacgctgg
    42241 gggaaatgaa cggtgacgcg gaactacgcg gtagcggtaa ctcggtggcg gcgcttttag
    42301 gcaacagtaa cggcaacctg aaactgttga tgaatgacgg gctggtgagc cgcaacctga
    42361 tggagattgt tgggctgaat gtcggcaact acattgtcgg tgcgatattt ggtgacgatg
    42421 aggtgcgggt gaactgcgcg gcggcgaatc tgaatattgc caacggcgtg gcgcgcccgc
    42481 agatttttgc tttcgatact gagaacgcgt tgattaatgt taccggcacg gcaagttttg
    42541 cttcggaaca gctggatttg actattgatc cggagagtaa agggattcgg attatcacac
    42601 tgcgttcgcc gctgtatgtg cgtgggacgt ttaaaaatcc gcaggctggg gtgaaagccg
    42661 gaccgctgat tgcccgtggt gctgttgctg cggcactggc aacgctggta acaccggcgg
    42721 cggcgttact ggcactgatc tcaccttccg aaggggaggc taatcagtgt cggacgatat
    42781 tgtcgcagat gaagaagtga tgataattgc cggatgtctg acatccggca attaaacaga
    42841 tttagatttt tggcaaagtg tgctttgttc atgccggatg cggcgtgaac gccttatccg
    42901 gcctacaaat ccttgtgaat tcaatatatt gtatggaaat tcagtcctga taagcgtagc
    42961 gcatcaggct tttcacactt attacaacga ctgatgtcgc gtctcatggg tcagcagcag
    43021 ggcgattaac gtcaagccag ccatcgccgc cagatataac cccaccgcac ctaacccgta
    43081 gttagcctgc aaccaggctg cgatatacgg cgcaacggaa gccccgagaa tcgacgatac
    43141 gttgtaagag aacgatgctc cggtgtaacg cacttctgtc ggaaacagct ctggtaacag
    43201 cgcacccatc ggcccgaagg tcagccccat cagacttaac cccagcagca ggaaggcaaa
    43261 aaccagaatc gggttgccag aaccgagcag cgggttaaag gcgaacagcg cgaacaggat
    43321 gatcagcgtg gtgatgatca ccatgctttt acggcgacca aaggcatcag ccagtaatcc
    43381 ggcgactggc accatcacgc caaaaccaat aactgccatc atcagcatcc acaacacttc
    43441 gttacgcggc aggccaagcc caactggcgc ggcggcggta ctgaaggtca tagagtagac
    43501 cgtcatgatg taaaacagcg tataggttgc cagcataatg aacgtaccca gtacggttac
    43561 gcgaacatgt ttggtcagca gcgtacccag cgggatcttc acctgttttt tcgctttagc
    43621 gactttctca aacaccggcg actcatgcag cgacacgcga acatacaggc cgataatgac
    43681 cagcaccgcc gagaagataa atggcacacg ccagccccag ctcataaact gctcatcggt
    43741 cagtagccag gaaagcagca agaaagtgcc gttggcaaag aagaagccaa tcggtgcgcc
    43801 tagctgtggg aaggagccat acagtgcacg tttgcgcggt ggggcgtttt cagtcgccag
    43861 cagcgccgcg ccgccccatt caccgcctaa gcccagaccc tgaccaaatc gagccagcgc
    43921 cagcagcagt ggggcgaaaa taccaatcgt ggcatagccc ggcagcagac caatcaccac
    43981 ggtcgaaatc cccatcgtta gcaacgaggc gaccagcgtc gctttacgcc caacgcgatc
    44041 gccaaaatga ccaaaaacgg cagagccaat ggggcgcgcg acgaaggcga tggcgaaggt
    44101 ggcgagcgac tgtagcgttg ctgccgttgg atcgccctgc gggaagaaga tatgcggaaa
    44161 cacaataacg gccgcagtgg cgtaaatgta aaagtcgaag aactcaatgg ccgtgccaat
    44221 gagagaggcg acaaggactt tattacgcga gttgatcggc gtgtattctt gttcgtggtc
    44281 gagtgttgtg gctgttgctt gcataatctt ttcttatttt ttggctaacg aatagccata
    44341 ttacgcacag caaaagtgat atttcaatgt gtaacaaatc gcggaaatgg tcaaaaacca
    44401 ggcaaaaagg ggataatgtt taggccagtg atttttcttc tatgaaatgc ttcacataat
    44461 tttataaata gtggataaaa ctggtttcag gttaaataaa agttaccaac tggatttata
    44521 tgctggggtt atgagttgga gtgaaattgt ttgctcccaa atagatggct gaagaggctc
    44581 tggggaagag tttcaagagg ttatgtgcgc attatagccg gatgcgatgc tggcgcatct
    44641 tatccggcct acggggagcc aggccgaata aaatcgtatg ccggataagg cgtttacgcc
    44701 gcatccggcc tattttaagg ctgcgttttc cctggcattc gcgggtcgtc tttatattcg
    44761 gcggtggcaa tccacgccgc gcaaaacagc gtcaaacggg cgaagaagta gaaaaatgcc
    44821 atcagcccca gcacggaacc aaatgctgcg ccagaggggg atttcatcag cgatggcagg
    44881 gtgtaggtca tcacgatttt aatcacttca aaaccaatag cagccagaaa tgttccgcga
    44941 atcagggctt ttttgcgcgg acggtggcgc ggcaaccgcc agaagatcca gaagaaaagc
    45001 agatagttgg cgaagatgga aatcgccagg ccaatcaatc gccacgtcgg tttcagccac
    45061 tcaatgctgt tcaggtgcag ggcgctaata atcatttgct gcgccgaacc ggcaaccgag
    45121 gtgatcgaaa gcgtcacaat cagcgcaatc aacaaaccaa tcagtgaaat aaaatcacgc
    45181 agatatttta cccagaactt ctcctgatct tgcggcgagc gttcccagac atcgcgcgac
    45241 tgggcacgaa tcgcttcacg caggttaccc atccagttga tgccggaata aagtgccacc
    45301 gccaggccga caagccctac agtcgtacgc tgctgaacgg cggtgttgat ggtgtttttc
    45361 aacgtggcgg ctagtgtcgg atcgctgatg ttttgcagaa ttttgtcgaa gatatcctgt
    45421 agcaacatcg gatgggaggc cagcacaaag ccccctgcgg caaacgacac catcaaaatc
    45481 gggatcatcg acaaaaatga gaaataggtg atagccgcac caaactggtt acccagccga
    45541 tcgttaaagc gttctgtcgc gcgaataagg tgcgcaataa ccggttggcg ttggactttt
    45601 tcggcagtgg tggtgacggt ttccagcgcc tggctaactt tgccgccttt ctcgctatta
    45661 tccaactgct taatcggctc gtgctccaga tcctgggtgg gacgtttgat ctcgttttcc
    45721 tgcgtcatcc gctcttttgt ccttctgtcg tggtcattct taaaaagtat agtcggtcag
    45781 tccaggtgct ctttcattac gccagccagc cactgcataa acaggtttac acgccgggaa
    45841 agctcccgac gctgcgggta aaccagcgaa agggagagcg gctcggcacg gtagcctggc
    45901 aacacttcaa taagccgccc ggcacgcagg gcttcgcgca cggcaatgcg cgggatctga
    45961 ataatcccca gaccggcaag accggcggcg agatagtttt cgctactgtt taccgtcaac
    46021 atgccgccag acttaaacca ctggacgcca ttaacgctgg caacctcaaa acctaacgga
    46081 tgtacaccca ggtgcggtgt ataacgcact attgcgtgtg aagtcagatc atcggggctt
    46141 tgcggataac caaagcgcgt cagatagtgc ggactggcac agttgaccat ggttagtttg
    46201 ccgaggggac gggcgataac gccatcttcc ggtaacgcgc cagtgcgtat cacgcaatca
    46261 aaaccatcat gaagaatatc taccggacgg ttatggctac tcagttccag ctcaattccc
    46321 ggatgctgat agagaaattc cgacagacgc ggcagtaaca ggtttttcgc gattcctgac
    46381 gggatgtcga tgcgtaattt accgctgata ctggtggcat cctgttgaaa cagaccgtcc
    46441 agttcgctga gattactcaa cacatctttt gcccgttgat aataggtcat gccttctggc
    46501 gtgagtttga cccgtcgcgt agtgcgctgg agaagctggg tgccaagttg atgttccagt
    46561 gcctgtatct ggcgcgaaac acttcccttt ggcaaagcaa agaaatccgc cgcgcgggaa
    46621 aaactttcca gctccgcgac tttgatgaac aactgcattg cgtgaatttt atccatttct
    46681 ttgaccgatt gttgttcaca gcgaaacagt aacgcgcata tcgcattgtt tattgttttt
    46741 tctaacatct aatgccgggt ttcacaatgt tgtcatgacg aatgtggcga tttctcagca
    46801 ggaaaagggc ggaatagctt tatgagaaag gtgaatcccc ttaaacagag cgtatttttc
    46861 agtgattccc tttaaagtga atagaggacg tattggctat ttgtgcttat ttttgtctca
    46921 ttgccatttg ttattttggc ttgtagtcgc ggaatttcat ttttaattgc atgcgtacta
    46981 accaccaata taaggaaatc ctcaaaattg ttttaaatag tgccctcgac atgtaatttc
    47041 aggcgtttag ctgtaactat ttatatcggt aactattttc tacgcgtcat ctctgtagaa
    47101 tggccaccgc caaatggcga attcttattt tttctcgcgg gtaataccct tccactactt
    47161 aagtcttttt aacgtattca ggtggagatt cagttatgca aatagtcatg tttgacaggc
    47221 agtcaatatt tattcatgga ataaaaatca gtttacagca gcgtattcca ggagtgagta
    47281 ttcagggggt cagtcaggca gacgagttat ggcaaaagct ggaaagttac cctgaagcct
    47341 tagttatgct cgatggcgat caggatggtg agttttgcta ctggttgctg caaaaaaccg
    47401 tggtgcagtt tcctgaggtt aaggtgttaa tcacggcgac ggattgcaat aaacggtggt
    47461 tacaggaagt tatccatttt aatgtgctgg ccattgtgcc tcgcgattca accgtcgaga
    47521 catttgcgtt ggcggtaaat agtgcggcga tggggatgat gtttctgcct ggagactggc
    47581 ggacgacgcc ggaaaaggac attaaagatc tcaaatcgtt aagcgcccgc cagcgggaga
    47641 ttttaaccat gttagcggcg ggcgaatcaa ataaggagat cggcagagcg ctgaatatca
    47701 gtaccggaac ggtaaaagcg catctggaat ccctgtatcg ccgtctggaa gtgaaaaatc
    47761 gcacccaggc ggcaatgatg ttaaatatct cctcctg
//
LOCUS       DAEBXN010000022        47466 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.022, whole genome shotgun sequence.
ACCESSION   DAEBXN010000022 DAEBXN010000000
VERSION     DAEBXN010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47466
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.022"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            172..654
                     /gene="sseA"
                     /locus_tag="KJL99_001230"
                     /pseudo
     CDS             172..654
                     /gene="sseA"
                     /locus_tag="KJL99_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
     gene            complement(1473..2249)
                     /gene="sseB"
                     /locus_tag="KJL99_001231"
     CDS             complement(1473..2249)
                     /gene="sseB"
                     /locus_tag="KJL99_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HBD4436020.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(2391..3674)
                     /gene="pepB"
                     /locus_tag="KJL99_001232"
     CDS             complement(2391..3674)
                     /gene="pepB"
                     /locus_tag="KJL99_001232"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HBD4436021.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAIMRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(3733..3933)
                     /gene="iscX"
                     /locus_tag="KJL99_001233"
     CDS             complement(3733..3933)
                     /gene="iscX"
                     /locus_tag="KJL99_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HBD4436022.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(3945..4280)
                     /gene="fdx"
                     /locus_tag="KJL99_001234"
     CDS             complement(3945..4280)
                     /gene="fdx"
                     /locus_tag="KJL99_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HBD4436023.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(4282..6132)
                     /gene="hscA"
                     /locus_tag="KJL99_001235"
     CDS             complement(4282..6132)
                     /gene="hscA"
                     /locus_tag="KJL99_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HBD4436024.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDATIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(6149..6664)
                     /gene="hscB"
                     /locus_tag="KJL99_001236"
     CDS             complement(6149..6664)
                     /gene="hscB"
                     /locus_tag="KJL99_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HBD4436025.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSLAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(6760..7083)
                     /gene="iscA"
                     /locus_tag="KJL99_001237"
     CDS             complement(6760..7083)
                     /gene="iscA"
                     /locus_tag="KJL99_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HBD4436026.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(7100..7486)
                     /gene="iscU"
                     /locus_tag="KJL99_001238"
     CDS             complement(7100..7486)
                     /gene="iscU"
                     /locus_tag="KJL99_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HBD4436027.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(7514..8728)
                     /gene="iscS"
                     /locus_tag="KJL99_001239"
     CDS             complement(7514..8728)
                     /gene="iscS"
                     /locus_tag="KJL99_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBD4436028.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(8840..9328)
                     /gene="iscR"
                     /locus_tag="KJL99_001240"
     CDS             complement(8840..9328)
                     /gene="iscR"
                     /locus_tag="KJL99_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HBD4436029.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(9598..10338)
                     /gene="trmJ"
                     /locus_tag="KJL99_001241"
     CDS             complement(9598..10338)
                     /gene="trmJ"
                     /locus_tag="KJL99_001241"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HBD4436030.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            10457..11260
                     /gene="suhB"
                     /locus_tag="KJL99_001242"
     CDS             10457..11260
                     /gene="suhB"
                     /locus_tag="KJL99_001242"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HBD4436031.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAILANMRDELSDALKH"
     gene            11405..12259
                     /locus_tag="KJL99_001243"
     CDS             11405..12259
                     /locus_tag="KJL99_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBD4436032.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQQLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGNVYREQMVDFI
                     LSALNPQN"
     gene            12450..13730
                     /gene="csiE"
                     /locus_tag="KJL99_001244"
     CDS             12450..13730
                     /gene="csiE"
                     /locus_tag="KJL99_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HBD4436033.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQHVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYIHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(13722..14861)
                     /locus_tag="KJL99_001245"
     CDS             complement(13722..14861)
                     /locus_tag="KJL99_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HBD4436034.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLPLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQVSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(15021..15911)
                     /gene="hcaR"
                     /locus_tag="KJL99_001246"
     CDS             complement(15021..15911)
                     /gene="hcaR"
                     /locus_tag="KJL99_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HBD4436035.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KLALRDFIAIVQERLASVTA"
     gene            16047..17408
                     /locus_tag="KJL99_001247"
     CDS             16047..17408
                     /locus_tag="KJL99_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HBD4436036.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNSRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLHDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            17405..17923
                     /locus_tag="KJL99_001248"
     CDS             17405..17923
                     /locus_tag="KJL99_001248"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HBD4436037.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            17923..18243
                     /locus_tag="KJL99_001249"
     CDS             17923..18243
                     /locus_tag="KJL99_001249"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HBD4436038.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            18240..19052
                     /gene="hcaB"
                     /locus_tag="KJL99_001250"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             18240..19052
                     /gene="hcaB"
                     /locus_tag="KJL99_001250"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HBD4436039.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTTSK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            19062..20269
                     /locus_tag="KJL99_001251"
                     /pseudo
     CDS             19062..20269
                     /locus_tag="KJL99_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
     gene            20366..20788
                     /locus_tag="KJL99_001252"
     CDS             20366..20788
                     /locus_tag="KJL99_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HBD4436040.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(20836..21708)
                     /locus_tag="KJL99_001253"
     CDS             complement(20836..21708)
                     /locus_tag="KJL99_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HBD4436041.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDEHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(21720..22814)
                     /locus_tag="KJL99_001254"
     CDS             complement(21720..22814)
                     /locus_tag="KJL99_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HBD4436042.1"
                     /translation="MKINRIVNEGFMKTMLAAYLLGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGGMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYVLMASGKCGKVVINFPD"
     gene            complement(22847..23845)
                     /locus_tag="KJL99_001255"
     CDS             complement(22847..23845)
                     /locus_tag="KJL99_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBD4436043.1"
                     /translation="MYASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(23870..25381)
                     /locus_tag="KJL99_001256"
     CDS             complement(23870..25381)
                     /locus_tag="KJL99_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBD4436044.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASY
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(25404..26386)
                     /locus_tag="KJL99_001257"
                     /pseudo
     CDS             complement(25404..26386)
                     /locus_tag="KJL99_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
     gene            complement(26483..29584)
                     /locus_tag="KJL99_001258"
     CDS             complement(26483..29584)
                     /locus_tag="KJL99_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HBD4436045.1"
                     /translation="MWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPALV
                     GMLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMTGF
                     TLRPDRAALEIASRVYNGNATPRHFLWWVNPAVKGGEGHQSVFPPDVTAVFDHGKRAV
                     SAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLHVA
                     NHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEEKR
                     FEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCNAL
                     LDDAVALTPATAIQGVLHGINPDRLTIELSDADGNIVLSYQEHQPQELPLPDVAKAPL
                     AAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNRAD
                     FPQAVAYASQALKRAHALNKKPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGNSK
                     AGGYYGLARLAARNDNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARLQR
                     EKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTLAA
                     EMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESIEE
                     CWFARHLLACFYYNKRSYNKAIALWQRCVEMSPEFADGWRGLAIHAWNKKHDYELAAR
                     YLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLNLW
                     HLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELLHA
                     ALYYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHSYY
                     NDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLLSL
                     YGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFIFN
                     YVH"
     gene            complement(29591..29764)
                     /locus_tag="KJL99_001259"
     CDS             complement(29591..29764)
                     /locus_tag="KJL99_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4436046.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKS"
     gene            29882..31075
                     /locus_tag="KJL99_001260"
     CDS             29882..31075
                     /locus_tag="KJL99_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBD4436047.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDVPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVLVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGSLETVASLSALKKQARVWLK
                     SQPVNTQLEPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(31273..32526)
                     /gene="glyA"
                     /locus_tag="KJL99_001261"
     CDS             complement(31273..32526)
                     /gene="glyA"
                     /locus_tag="KJL99_001261"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBD4436048.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            32853..34043
                     /gene="hmpA"
                     /locus_tag="KJL99_001262"
     CDS             32853..34043
                     /gene="hmpA"
                     /locus_tag="KJL99_001262"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HBD4436049.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISTGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGEVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(34088..34426)
                     /gene="glnB"
                     /locus_tag="KJL99_001263"
     CDS             complement(34088..34426)
                     /gene="glnB"
                     /locus_tag="KJL99_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HBD4436050.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(34487..35821)
                     /gene="glrR"
                     /locus_tag="KJL99_001264"
     CDS             complement(34487..35821)
                     /gene="glrR"
                     /locus_tag="KJL99_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HBD4436051.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNHFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(35811..36524)
                     /gene="qseG"
                     /locus_tag="KJL99_001265"
     CDS             complement(35811..36524)
                     /gene="qseG"
                     /locus_tag="KJL99_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HBD4436052.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(36689..38116)
                     /gene="qseE"
                     /locus_tag="KJL99_001266"
     CDS             complement(36689..38116)
                     /gene="qseE"
                     /locus_tag="KJL99_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HBD4436053.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSCNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(38674..42561)
                     /gene="purL"
                     /locus_tag="KJL99_001267"
                     /gene_synonym="purI"
     CDS             complement(38674..42561)
                     /gene="purL"
                     /locus_tag="KJL99_001267"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HBD4436054.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYNFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLCTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVVQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFQRGGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            42820..44376
                     /gene="mltF"
                     /locus_tag="KJL99_001268"
     CDS             42820..44376
                     /gene="mltF"
                     /locus_tag="KJL99_001268"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HBD4436055.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(44373..44909)
                     /gene="tadA"
                     /locus_tag="KJL99_001269"
     CDS             complement(44373..44909)
                     /gene="tadA"
                     /locus_tag="KJL99_001269"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HBD4436056.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(44934..45569)
                     /gene="yfhb"
                     /locus_tag="KJL99_001270"
     CDS             complement(44934..45569)
                     /gene="yfhb"
                     /locus_tag="KJL99_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HBD4436057.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            45778..46626
                     /locus_tag="KJL99_001271"
     CDS             45778..46626
                     /locus_tag="KJL99_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBD4436058.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAALRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            complement(46965..47466)
                     /locus_tag="KJL99_001272"
                     /pseudo
     CDS             complement(46965..47466)
                     /locus_tag="KJL99_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT        11320 a        13012 c        11939 g        11195 t
ORIGIN      
        1 acgttacaga cggtcccggc aaaagtgcct gacctgaccc ctattagcgg ttaccagcgc
       61 cccaccagac ccggtgacat ccccccccgg atcatggacg actgggggca gtaatcatgc
      121 cgggtctggc tggctaacac cccattataa ggggtacgaa taaagtaacg gggacttgca
      181 ggctggcagc gcgatgacct gctattagaa gaaggtgcag tagagctgcc ggaaggagag
      241 tttaacgccg cgtttaatcc tgaagcagtg gtgaaagtaa ccgatgtgtt attggcaagc
      301 catgaaaata cggcgcaaat tattgatgcc cgcccggctg cacgttttaa cgcagaagtt
      361 gatgaacctc gcccaggttt acgtcgcgga catattcccg gtgcattgaa cgttccgtgg
      421 acggaactgg tgcgcgaagg cgaactaaaa acgaccgatg aactggatgc gatatttttt
      481 ggtcgcggcg tcagctatga caaaccgatt atcgtcagct gcggctctgg tgtaacggca
      541 gccgtggttt tgttagcact cgcgacgctg gatgtgccaa acgtgaaact gtacgacggc
      601 gcatggagtg aatggggcgc gcgggcagat ttaccggttg agccagtgaa ataagtatat
      661 tacaggcaat aaaaaaccgc cgaatttggc gtttttttat tgctggtctg gttcgcggcc
      721 tttccagcag gttgacttgt gttacatgag caacgcaggt gcttcacagc aaaacaatac
      781 tcaccagtaa ctctcttttt gtcaagcaaa agagagtaat tattgtttat ttagcgtatt
      841 atcgacaccg gccctttccg ccgtgttcgg taataaaata acctggctta ttagtccgaa
      901 ttcagacaaa tataaacaaa tcctgctcaa aattaaaaat tctaaccggt aaaagatatt
      961 acttaaacat gtaaatttac tttcctttaa aaaacaaaaa accgccaaaa tcaggcggtt
     1021 atttgttgct ggtccggttc gcggcctttc cagcaggttg tattaccgta gtaatgcaag
     1081 cgcgtctcag cggagacaat actcgccagt aactctcttt ttgtcaagca aaagagagtt
     1141 attattgttc tgttagtgta ttatccactg cggccctttc cgccgtctcg caagcgggcg
     1201 ctggcttaag gaaaggatgt tccgcgaccg ttagagcaag cgcgtctcag cgcttgctat
     1261 cgcggcaata tcgccagtgg tgctgtcgtg atgcggtctt cgcatggacc gcacaatgaa
     1321 gatacggtgc ttctgtatcg tacttattgt ttctggtgcg ctgttttccc aagtaaataa
     1381 taaccggagt ctctccggcg acaatttact ggtggttaac aaccttcaga gcagcaagta
     1441 agcccgaatg ccgcccttta gggcggcata ttttagatta tccggttttg tttaaagtca
     1501 cgcaaaaaac caccccagcg acgttcatag aatggcgcaa tatgttcggt aataaagtgg
     1561 ctaattcctt tttccccttt tttcacctga caaatatcga ttggttcatc gccaggtaat
     1621 gtatcggtcg ctacacttcc cgtcgcctga ataatttctt cgatatcacc atcggcttca
     1681 atgccaataa gtaaattagg ctgtgcctct tcgttctctt taattgaaca aataaaagca
     1741 cgcttcaccg gcttaatggt tttaaataag gtggtgagtg aatcaatcat ttgtgctggc
     1801 ggctctgcga cttccgataa tatcagcgat tcaccgcctt ccaggatttc ctggctgctc
     1861 agcggatttc cctcttcacc aatcaacaaa ctgatttcac gcggcataaa ttctttaccg
     1921 gtcggcagtt tggcattaag gaagagcgtt tcgccaaggg tcatctcaaa caatgtgcga
     1981 acgggcatta cgacaaatgc ctgttcgtct tcaaccgcct gttgaagcgc ttctaacgag
     2041 gtgaaaaaag gaatgacgct ggtgccgtct tctttttccc agtgctgtaa atcaagcgca
     2101 ctatcttcaa ccacagcctc gccctgcgcc gccgtaccag gcacccagac ggtggattcc
     2161 agtagagtac ggaaaaaggc cgggcggtgc gccggttcgg ttgctgcttt ttccagcagg
     2221 tcttcaagtt cgttttttgt ttcggacata agaaccacaa ttcattcaac gttcggggcg
     2281 caaatgcctg atgcgctacg cttatcaggc ctacaaggga ttcgcaattt gttgaatttg
     2341 cagaatttgt aggccggata aggcgtttac gccgcatccg gcatattagt ttacgccgtt
     2401 aacagattag ctatcgtgcg cacaccaagt cccgtagcgc ccgcagacca ctgttcaacc
     2461 ggcgctttac ggtaagtcgc cgagcagtcg atatgcagcc agccttgctg atagttctca
     2521 acaaagtgcg acaggaagcc cgccgccgtg ctcgcgcctg ccggatacgc cgcgcttccg
     2581 gtattgttca gttcggcaaa gttagacggc agctggctgc ggtggaattc cgccagcggc
     2641 agacgccaga atggttcgtt ctcttgtgag gcactcgcca gtaaacgacc ggcaagcgca
     2701 tcgtcaaaac tgaacagcgc gtgataatca ttacccagcg cagttttcgc cgccccggtg
     2761 agggtcgccg catcaatgat catttccggt ttctgcgcac tggcatcaat cagaccatcg
     2821 gcaagcacca gacgcccttc ggcatcagtg ttcatcactt caactttttt accgttgcga
     2881 taggtgatga tatcgcccag cttgaacgca ttgccgctaa tcaggttatc cgcacagcag
     2941 aggaacagct tcacgcgctt gttcagtccg cgcataatgg caaatgccag cgccccggta
     3001 accgttgccg cgccgcccat gtccgacttc atcgagtcca taaacgcagt ctgtttgatg
     3061 ctgtagccgc cggagtcaaa agtgatacct ttacctacca ggcacgcgta caccggtgct
     3121 tctttatcgc cagttgggtt gtaatccagc gccagcaata ccggagaacg ttctgaaccg
     3181 cgcccgacag tgtgcagccc catataacct tgctcacgca gatcttcgcc tttggtgatc
     3241 cgataagtca cacgatcgcc cgcgacgttg ctgatcagat caacagcacg ctgtgccagt
     3301 tgcgatggtc ccaattcttc tgccggtgcg ttgatggtgt cacgcaccca gtcgatgatc
     3361 atcaggcggt tatccagttc ctggcgctgg gcatcgtcca gatccggcca ctccacttta
     3421 cgcgtgcctt tcgggccttt ataaccttgc cagaatgccc agcagcgatc cgcatcccag
     3481 ccttcaccgc ttaactgaac atgcttgatg cccagaccgt caatcttgcg ggccgcacgc
     3541 tggatcagcc ccagatcgtc tgccccgttc aaatgcaggg taatgccgtc attattaatg
     3601 ctgtaagttg ctttttctcc ccagcgcgcg tcggcaggtt gggtagagag ggtaatcttc
     3661 atcgcttctg tcattttagt tatccttatt cgtttaacag caaaacgggc tacccccagg
     3721 cagcccgttc attcactcgg cttcgtccag ccagactaac aaaatcgctt cgaggatttt
     3781 ctcgttggat gcctgcgggt cgtcgtcgaa atcttccaga tcgcaaatcc actgatgcat
     3841 atcggtgaat cgaaccgttt tcggatcaag atcgggatac gcatcgtaca gtgcttcgcc
     3901 aatttcgcgg ctatcggtcc acttaagtcc catactaacc tctgttaatg ctcacgcgca
     3961 tggttgatag tgtaacgcgg gatttcgacc actaaatctt cgtcggtgac gcgcgcctgg
     4021 cagcttaaac ggctttccgg ctccagtccc caggctttgt ccagcatgtc gtcttcctgc
     4081 tctgagcttt ccggcagtga gtcaaaacct tcacgaacga tgcagtggca ggtggtgcaa
     4141 gcacaggatt tttcacaggc gtgttcaatc tcaataccgt tacgcagcgc tgcgtcgaga
     4201 atggtttcac cgctattagc ttccagaaca gcgccatcag ggcagaggtc ctgatgaggc
     4261 aaaataacaa tctttggcat attaaacctc gtccacggaa tggcctttca gcgcacgacg
     4321 aaccgactgg tccatgcggc gagcggcgaa atcctgggtt tgtttgtcta cgtttttaat
     4381 cgcttgttcg atggcgtcaa catcatcgcc ctgcgccact tcactcaggt gagcggcagc
     4441 attgtcaatg acctgacgct ctgcggcgct taacagcgcg gcatcagcag ccagcgcgcc
     4501 gtgcagactt tccagcacac gcgccgcttc tactttttgt tctgccagca tacgggcttt
     4561 tacgtcctgc tcggcatagc tcattgagtc tttgatcatc gaagcgattt cgctatcggt
     4621 cagaccgtaa gacggtttga cctgaataga cgcctcaacg ccggtggatt tctccatcgc
     4681 cgtcacgctc aaaagaccgt cggcatcgac ctggaacgtc acgcgaatat gcgcaccgcc
     4741 agccggtagc gccggaatac cacgcagcgc aaaacgcgcc agtgagcggc agtcctgcac
     4801 cagttcgcgc tcaccctgca ttacatggat agacatcgcc gtctgaccat ctttaaaggt
     4861 ggtgaaatcc tgagcgcggg ccaccggaat agtggtatta cgcggaatca ctttctccac
     4921 caggccgccc atcgtttcga ggcccagcga cagtgggatc acatcaagca gcagcatttc
     4981 gctgtctggc ttgttaccca ccagaatatc cgcctgaatc gccgcgccaa tagcgacgac
     5041 tttatccggg tcgatggaag tcagcggtgg acgaccgaaa aattcgccta cccgttcacg
     5101 caccagcggc acgcgagtag aaccgcccac catcaccact tccagcactt cgtcagcttc
     5161 tacacccgcg tctttcagcg cgcgacgaca agccagtaag gttcgtttta ccagtggcgc
     5221 gatcagttca ttgaattgtt cacggctgat ttcgccctgc cagcccgcaa cgttaacggt
     5281 cacggagtcc gcatcgctca gcgcgatttt ggctgcaatg gtggcatcca gcagttcacg
     5341 ctgaacgcgg ttatcgctac gatcaggaat gtccgcctgc tcgcgaatgt aatccgccag
     5401 cagatggtcg aaatcatcgc cgccgagcgc ggaatcaccg ccggttgcca gcacttcaaa
     5461 cacgccgcga cttaagcgca gaatggaaat atcaaacgtc ccgccaccga ggtcataaac
     5521 agcgatcacg ccttcctgac cggaatccag cccgtaggcg atagccgcag cagtcggttc
     5581 gttaagtaag cgcagaacat gcaaccccgc cagacgcgcc gcgtctttgg tgccctgacg
     5641 ctgggcatcg tcaaagtacg ccggaacggt gataactaca ccatccagct cgcctgccag
     5701 ggcttcagtt gcccgcgccg ccagtgcttt gaggatgtcc gcagaaacgc gcaccgggtt
     5761 cagtagcccc gccgccgttt caatcatcgg caggccgttt tcgctggcct ggaattgata
     5821 aggcagatgc ggatagcgtt gctggatatc agccagcgag cgccccatca ggcgtttaac
     5881 agaactaatg gtgttggcgg tgtcaagcgc tgcgttagta cgcgcgtcat aacccaccga
     5941 atgcccttgc tgttgatagt gaacaacaga tggcagcagg tgacggcctt catggtcggc
     6001 taacgtttcg gcctgaccgc tgcgcactgt cgccaccagc gagttggttg tgcccaggtc
     6061 aataccagcc gccagacgac gctgatgcgg cgcagcactc aaaccaggtt cactaatttg
     6121 taataaggcc atgtttagct tccagaaatt aaaaatcgag cagtttttct tcgagttgtt
     6181 cggcactgct ccgcagttta tcgagaaaac gcagcttacg caccgtatcc gccgccgcgt
     6241 cccacgcctc gttgtctaac tgttcaacca tcaactgatg gcgggtatca aacatctttt
     6301 tcacacgttt gataaagctt tccagccgcg cttcatcttt cgcctgttcg atctcgtcca
     6361 gctcttcgcg cagctccaac tgttccatca ggaacgcggt gtcgcgcaca gtatgctgct
     6421 cgctggcgag atcaaaaccg tgtaaagaaa gcaaatattc cgcgcgcatt aacggatgac
     6481 gcagcgtttg ccaggcctgg ttaatggttg cagattgctg tacggcggcg agttgttccg
     6541 ccaggcttcc gctggcgaat ttatcaggat gatactgacg ttgtagatcc tgaaaacgca
     6601 ggctcagtgc ctgggtatcg agttgataac gggcaggcaa gccaaagagg gtgaagtaat
     6661 ccataacatt ctcagggctg cgatccgccg cgataacggc gaatcaggta aaacaaaccc
     6721 cacgcgcagg cgaccacggt ggggttatcg gtatgcgcat caaacgtgga agctttcgcc
     6781 gcaaccacac tcatctttaa cgttcgggtt ggtgaattta aacccttcat tcaggccttc
     6841 ttttacgaag tccagctgcg taccgtccag aaattgcagg cttttgccat cgaccacgac
     6901 tttcacgcct ttgtcttcaa acacgatgtc ttccggcgtc ggttcgtcaa caaattccag
     6961 tacataagcc atacctgaac atccggaggt tctcacgccc agacgcaggc caaacccttt
     7021 gccgcggtta gccagaaagg tatttactcg cgctgctgca ctgtcgctca gtgtaatcga
     7081 cataaccaaa cctcaactct tattttgctt cacgtttgct tttatagtcc gcaatggcgg
     7141 ctttgatcgc gtcttctgcc agaatagaac agtgaatttt caccggcggc agttcaagtt
     7201 cttcagcaat atcggtgttt ttgatcgcct gcgcttcgtc gagagacttc cctttcaccc
     7261 attcggtgac cagggagctg gaagcgatag cggaaccgca gccgtaagtt ttaaaacgcg
     7321 cgtcttcaat gataccttca tcgttgactt taatctgcaa cttcattacg tcgccacagg
     7381 ccggtgcacc caccatgccg ctgccgacgt tctcgtcgtt gttatcaaag gaacccacgt
     7441 tacgcggatt ctcgtaatgg tcgataactt tttcgctgta agccattata aattctcctg
     7501 attccgatac cgattaatga tgagcccatt cgatgctgtt cagatccacg ccctgcttgt
     7561 acatttccca cagcggagaa aggtcacgca gacgaccgat ggatttacga actaactcga
     7621 tggtgtagtc gatctcttct tcagtagtaa aacgacctaa agagaaacgg atagagctat
     7681 gtgccagctc gtcgttcagc cccagcgcgc gcagcacgta ggacggttcg aggcttgctg
     7741 acgtacaggc ggaacctgaa gaaactgcga ggtctttcag cgccataatc agcgactcac
     7801 cttcaacgta gttgaagctg acgttgagaa tgttcggcgc accgtgttcc aggtcaccgt
     7861 tcaggtaaac ttcttcgata tctttgatgc cgttccacag acggttacgc aggccgcgca
     7921 gacgttccat ctcggtcgcc atctcttctt ttgcgatgcg ataggcttcg cccattccga
     7981 cgatctggtg aacaggcaga gtgccggaac gcataccgcg ctcgtgaccg ccgccgtgca
     8041 tttgcgcttc gatgcgtaca cgcggtttac gacgcacata cagcgcaccg atacctttcg
     8101 ggccatagat tttgtgaccg gagaaagaca tcaggtcaac tttcaactgg ctcaggtcga
     8161 ttggcagttt gcccacgctc tgggttgcat caacgtgata gataatgcca cgagcacggc
     8221 acatttcgcc gatagccgcg atatcctgca ccacgccgat ttcgttattt acgtgcatga
     8281 tggaaacaag gatggtgtcg tcacgcatcg ccgcttcgag ttctttcagg tcgataatac
     8341 cgttacgctg cggtgccagg taggtgactt cgaaaccttc gcgctccagc tgacggcaag
     8401 tatccagtac cgctttgtgt tcggttttgc tggtgatgat gtgcttgcct tttttctgat
     8461 aaaagttggc tgcacctttg atcgccaggt tgtcagattc ggttgcacca gaggtaaaga
     8521 cgatttcacg cggatcagcg ccgaccagat cggcaatctg attacgggcg atatctaccg
     8581 cttcttcagc ctgccagccg aaacggtgag aacgagaggc cgggttacca aaggttccgt
     8641 ccatcgtcat gaactgcatc attttctcgg caacacgcgg gtccaccggc gtggttgcgg
     8701 agtagtcgag ataaatcggt aatttcattg ctctataaac tccgtacatc actcaatgca
     8761 aggaatcagg ctaccggctg gatgtacgac cgtgtttacg gagtatttag cactccggcc
     8821 tgattctgaa ttctttttat taagcgcgta acttaacgtc gatcgcgtct tgtgtgcggg
     8881 tgcgtggcgc gtcgtgagta tgctgacgac cagacacatc cagcacttcc tggttattaa
     8941 ccagttcgcc taaagtaatg ttgttgagaa aaccggtgag acggtcgctc aaatcacgcc
     9001 acagcgcgtg ggtcaggcat ttatcgccgc cctggcagcc gcctttaccc tgacaacggg
     9061 tggcatctac agattcgtca acggcgctaa tcacttcgcc aacggcgatg ctgctggcat
     9121 ctttgcctaa cagataacca ccgcctggtc cacgtacgct ggaaaccaga ccatttttac
     9181 gcagacggga aaacagttgt tccagataag aaagggaaat tccctgacgt tcggaaatat
     9241 cagccaacgg taccgggccc gcttcagagt tgagcgcaac gtcaagcatt gcggtcacgg
     9301 catagcgccc tttagatgtc agtctcatgt cttacttcac ctcaaactcg cccctgcccg
     9361 gggtttttta ttgtaaaagt gggggtattg catagcaggg tcaagtctga cattcccgag
     9421 taaattggtc aactatttac ttgactgatt tagtcgggta tttaaccttc agtgccattt
     9481 ttttatcgtg gcgtttgtac ctgttgtcgg atgcggtgtg aacaccttat ccgacctacg
     9541 attctgtaca ttgtaggcct gataagacgc gttaagcgtc gcatcaggca accactgtta
     9601 ttcggccttg ttacctttat tctgctgctc aatagaagcc agaatcccgc gcaggatatt
     9661 caattcctgg ctttccgggc gcgcacgggt aaacagacgg cgcaatttat tcatcacctg
     9721 ccccggatgg ttttcacgga taaaaccggt tgccagcagc gtttgttcca gatgaccata
     9781 aaaacgctcc agatcatcga ccagcggata cggcgtctct tcatgctcga cctgctcgcc
     9841 attttcctga gttgccagcc aggccatacg cacttcatag gcaataacct gaaccgccat
     9901 tgccaggttc agcgaactgt attccgggtt tgccgcaatc gcgacatgat aatggcattt
     9961 ctgcaactct tcattggtca agccgacgcg ctcacgacca aacaccagcg ccaccggggt
    10021 atttgccgct tcagcgacgc ttttcagacc gcattcgcgc gggtcgagca tcggccacgg
    10081 cagcgtgcgg gagcgtgcgc tggtgcccac caccaggcta caaccggcta acgcttcgtc
    10141 gagcgtatcg acgatgtgag cattaccaat cacatcgctg gcccctgcag ccagggcaat
    10201 cgcctgggag tcgggtttca ccagtggatt aaccagccac agattggtta atcccattgt
    10261 tttcatggca cgggcaacag aacccatatt gccggtgtgt gacgtctcca ccagcacaat
    10321 tcgaatattt tgcagcattg tctttcttca tctaaagatt attcacgcat cttatcataa
    10381 aacgaagaca gatgccgatc tcgctgctat actctgcgcc gttttcccgt tctttaacat
    10441 ccagtgagag agaccgatgc atccgatgct gaacatcgcc gtgcgcgcag cgcgcaaggc
    10501 gggtaattta attgccaaaa actatgaaac cccggacgct gtagaagcga gccagaaagg
    10561 cagtaacgat ttcgtgacca acgtagataa agctgccgaa gcggtgatta tcgacacgat
    10621 tcgtaaatct tacccacagc acaccatcat caccgaagaa agcggtgaac ttgaaggtac
    10681 tgatcaggat gttcaatggg ttatcgatcc actggatggt actaccaact ttatcaaacg
    10741 tctgccgcac ttcgcggtat ctatcgccgt tcgtatcaaa ggccgcaccg aagttgctgt
    10801 ggtatacgat cctatgcgta acgaactgtt caccgccact cgcggtcaag gcgcacagct
    10861 gaacggctac cgtctgcgcg gtagcaccgc tcgcgatctc gacggtacca ttctggcgac
    10921 cggcttcccg ttcaaagcaa aacagtacgc cactacctac atcaacatcg tcggcaagct
    10981 gttcaacgaa tgtgcagact tccgtcgtac cggttctgcg gcgctggatc tggcttacgt
    11041 cgctgcgggt cgtgttgacg gtttctttga aatcggtctg cgtccgtggg atttcgcggc
    11101 aggcgagctg ctggttcgtg aagcgggcgg catcgtcagc gacttcaccg gtggtcataa
    11161 ctatatgctg accggtaaca tcgttgctgg taacccgcgt gttgtgaaag ccatactggc
    11221 gaacatgcgt gacgagttaa gcgacgctct gaagcattaa tgactcaggc gggtgatatc
    11281 actcacccgc cctcgccttt caggcgctat tccgaaatac ttcctcaccg ctttactttc
    11341 tttcgtcact ctcccaccat ctttccccga ttaatggatg ataaagaagt cgcaaggaaa
    11401 ggttatggca ctgccagtga acaaacgcgt tcccaaaatt ctgtttattc tctttgttgt
    11461 tgccttctgc gtttatttag tgccgcgcgt tgccatcaac ttcttctatt atcctgacga
    11521 taaaatttac ggtcccgacc cctggtcggc ggaatccgtc gaatttacgg ctaaggacgg
    11581 tactcgtctg caaggctggt ttattccttc ttcgacgggc cctgctgaca acgccatcgc
    11641 aaccatcatt catgctcacg gcaatgccgg aaatatgtcc gcccactggc cgctggtcag
    11701 ttggttacct gagcgtaatt tcaacgtttt tatgtttgat tatcgcgggt ttggtaaatc
    11761 aaaaggcact ccgtcccagg ccggattgct ggacgatacg caaagcgcca tcaatgtggt
    11821 gcgccatcgc agtgatgtaa acccacaaca gctggtgctg ttcgggcaga gtattggcgg
    11881 ggcgaatatt cttgctgtta ttggtcaggg tgatcgtgaa ggcatacgtg cggtgatcct
    11941 cgactccaca tttgcctctt atgcaaccat cgccaaccag atgatccccg gcagtggcta
    12001 cttacttgat gagagttaca gcggcgaaaa ttacatcgcc agcgtcagcc cgatcccgct
    12061 tttactcatt cacggtaaag ccgatcacgt tatcccatgg cagcacagcg aaaagttgta
    12121 tagcctggca aaagagccaa aacggttgat cctaatcccg gatggcgaac acattgatgc
    12181 tttttccgat cgtcacggca atgtttatcg cgaacagatg gtggacttta tccttagcgc
    12241 gttgaatccg cagaactaac ccatgatcgc tagcacgata atcattcaca aaaccacctt
    12301 aagacatgct aatccactgg tcagaacagt ttaaaatgag aaaaattctg tgacgcttgc
    12361 caacatttct gatgattagc attcccttcg ccatttccct gagcaaactt tagctattct
    12421 tatcaattat gcttatggga gatctacaga tgatgcctac gcttgctcca ccatctgtcc
    12481 tttcggctcc ccagcgccgc tgccagatct tgctgacgct ctttcagccg gggttgaccg
    12541 ccaccatggc aaccttcagc gagcttaatg gtgtggatga tgatattgcc agtcttgata
    12601 tcagcgaaac aggacgggag atcctgcgct atcatcaact cacactgacg actggttatg
    12661 acggtagcta ccgggttgaa ggtacagtgc ttaaccaacg tttgtgttta tttcactggc
    12721 tacgacgtgg tttccgtctg tgtccgtcat ttattaccag ccaattcacc cccgccctga
    12781 agagtgaact gaagcggcgc ggaattgcgc gtaactttta cgacgatacc aatctacaag
    12841 cgttagtgaa tctctgctcc cgacggctgc aaaaacgctt tgaatcgcgc gatattcatt
    12901 tcctgtgtct gtatctgcaa tattgtttgc tgcagcacca cgctggaatt acgcctcagt
    12961 ttaatccgct ccaacgtcgc tgggcggaat cctgccttga atttcaggta gcgcaggaaa
    13021 ttggacgcca ctggcagcgt cgggcgctcc agcatgtacc gcctgatgag ccactgttta
    13081 tggcattact tttttccatg ttgcgggttc ccgatccact gcgggatgcg catcagcggg
    13141 acagacaatt gcgtcagtct atcaaacgtc tggtaaacca ttttcgtgag ctgggaaatg
    13201 ttcgttttta tgatgaacag gggttatgtg atcagcttta tatccacctc gcccaggcgt
    13261 taaatcgcag tttgtttgcc atcggtattg ataataccct gccggaagag ttcgccagac
    13321 tgtatccacg cctggtacgc accacccgcg cggcgctggc cggatttgaa agtgaatacg
    13381 gcgtccatct ttctgatgag gaaagtggtc tggtcgcggt gattttcggt gcctggctaa
    13441 tgcaggaaaa cgatctgcat gaaaaacaga ttattctact gaccgggaat gatagcgagc
    13501 gagaagcgca gattgagcag cagttacgcg aactaacgtt actgccgctc aacattaagc
    13561 atatgtcggt aaaggcattt ttgcagacag gcgctccgcg tggcgcggca ctgattattg
    13621 cgccttatac catgccgtta ccgctctttt caccaccgct gatctatacg gacctgacgc
    13681 tgacaacaca tcaacaggag cagatccgca aaatgctcga atcagcatga gggaacaact
    13741 ttcgggcgca aaaacattgc cggaagcgcc accagcgcca ttacccagaa cacgccgtgg
    13801 cccagatatt gatacaggaa accggcgaaa acggtcatga tagcgatact gccgcccatc
    13861 gcgacggcag agtaaaccgc ctgtaaacgg atgacttcgc taacctggcg agcagcaata
    13921 taacgcatgg cggccaggtg gcagaccgtg aaggtgccgc aatgcagaat ttgcaccact
    13981 atcaaccacg gcaacgccgt agttgctccc ataatgcccc agcgcactac gccgcaaatc
    14041 gccgagatca acagcatatc gcgagcacta caacggcgga aaagtttatt actcagcgca
    14101 aagataatga cttccgccac cacgcccagc gaccacaaat accccaccgc cgaggccgag
    14161 tagccagctg cctgccagta aatggcgcta aaaccgtaat aggccgcatg tgccccctgc
    14221 aataaacaaa cgcaggccag aaagcgccag ttctggcgaa ccagcgccaa ccacgcagac
    14281 caaccggtgc tctcctgctg gcggcttgcc ccttgtggct gaatcgtcgg acggatgaga
    14341 aagccgagca gcatggatgc cacgcccaac gtcaacagca cgaggatcac ccgataatca
    14401 aacatagtga ccagtttgcc cgtcagcgcc gagccaatga caaacgccac cgagccccac
    14461 agtcgcactt tgccgtaatc aagcgggaac tgcttttgcc acgtattcgc cagtgcatcg
    14521 gtcaacggta ccagcggtga gaaaaagagg ttaaagccaa tcatcaccag catcagccac
    14581 gctacgtgcg cccccgccca gaaggcgaca gcaaagagaa gtggcagcag tgccagcacg
    14641 cgcaaggcgg aaatcaggcg ggaaggatcg ctgacgcggg gcgcgatgag caaactcccg
    14701 agaaaacggg caaccagacc tgcccccaat aacaggccga tggtttctgg cgttaaacca
    14761 atccctttaa gccagacgct ccagaaaggt aaaaaaatgc cgtaactaaa aaagtatgtg
    14821 aaatagccga gcgccaacca gcgcgtggat tgcaaaacca tgagtccctc ccgtcaggag
    14881 gcgttagtct ggggttaatc gaatgttggt gcaagttgaa aaataagaat attcgagcct
    14941 gcttcccgat gtctgtaacg ccagttcaca agcactaaaa aactgcgatc cgcgacgcat
    15001 gttgcaaaaa taatgcgtaa ttatgccgtt acgcttgcca aacgttcctg cacaatggcg
    15061 atgaaatcgc gtaatgccag cttcatctcc cctttcttcc acgccatcag taaagcaatg
    15121 gaaggcacat ttccggcaat gggacgaaaa acaacctgcc cggtattaaa attattcata
    15181 taaccgggta tcaaagtgac gcccagcccc atgcccacca gattcatggt caccagaata
    15241 ttcgttgcca cctggacgat atttggctgg ctgttttctt gcgcaaacca cgctttaacg
    15301 atcggcgcaa gcgaaccgga atacgccgga tcggtactga cgaaattcac gccatccagt
    15361 tgcgcggcgg tgatctcttt ttcatgtgct aaagggtgat caaccggtaa caccaccact
    15421 aatggttcgt caaaaagctc cagataatca atctccgggc tataaacagg atggcgcatt
    15481 aagccgacgt cgagttcacc acggcgaatt ttttcctcct gttgcgtggt gattaaactc
    15541 accagctcaa tcaaggtgtc tggctgtctg agacgaaaca tcggtaatac ttttggcaat
    15601 aaattcactt ccgccgatgg cacaaagcca atggttaatt gtctgtcttc ctgaacaatt
    15661 ttccgcgccc gtaatttggc attttccgct tgttcgagga ttgccagcgc gtcctggaga
    15721 aaacattctc ccgccgccgt caacgcgact ttgcgcttat ccctcaccag taacggaaca
    15781 ccgacacagt tttcaagatc gcggatctgg ctgcttaacg aaggctgtga ggtatgcagt
    15841 ttttccgccg cacgggtaaa gttcagtgcc tgcgccactg cgacgaaata gcgtaaatgc
    15901 cgtagttcca tcaccttccc cttgttatcg aaaaaacgtc tcagctggta gaaattaact
    15961 atttcacata ttagcaacca accagcaaca tccttatggc acaaaaatag aaggtcaata
    16021 catcttatct ttcaggatta aaaaatatga ccacaccctc agatttgaac atttaccaac
    16081 tgattgacac ccaaaacggt cgggtcactc cgcgtattta taccgacccg gacatttacc
    16141 aactggagct tgagcgtatt ttcggtcgtt gctggctatt tctcgcccac gaaagccaga
    16201 tcccaaaacc cggtgatttc tttaacacct acatgggaga agatgcagtt gtcgtggtgc
    16261 gccagaaaga cggcagcatc aaggcgtttc tcaaccaatg ccgccaccgg gccatgcgtg
    16321 tgagttatgc agattgtggc aacagtcgcg cctttacctg cccgtatcac ggctggtctt
    16381 atggcattaa cggcgagttg atcgatgtac cgctggaacc tcgcgcctac ccacaagggt
    16441 tgtgtaaatc ccactggggg ctaaacgaag ttccttgtgt ggagagttat aaaggactga
    16501 tttttggcaa ctgggatacc agcgcaccgg gcctgcatga ttacctgggt gacattgcct
    16561 ggtatctgga tggcatgctg gatcgtcgcg aaggcggcac cgaaattgtc ggcggcgtac
    16621 aaaagtgggt gatcaactgt aactggaaat tcccggcaga gcagttcgcc agtgaccagt
    16681 atcatgctct gttcagccat gcttctgccg ttcaggtatt aggggcgaaa gatgatggca
    16741 gcgataagcg cctcggtgat ggacaaaccg cccgcccggt gtgggaaacc gccaaagatg
    16801 cgctgcaatt tggtcaggac ggtcacggta gcggtttctt ctttactgaa aaaccggatg
    16861 ctaatgtctg ggtcgatggc gcagtttcaa gctattaccg cgaaacctat gccgaagcag
    16921 aacaacgttt aggtgaagtt cgcgccctgc gcctggcggg tcataacaat atattcccca
    16981 cgctttcatg gctaaacggc actgccacac tccgcgtctg gcatccgcgc ggccctgatc
    17041 aagttgaagt gtgggcgttc tgtattactg acaaagccgc ctccgatgaa gttaaagccg
    17101 cttttgaaaa cagcgccact cgtgcttttg gtcctgctgg ttttctcgag caggatgact
    17161 cggagaactg gtgtgaaatc cagaaattgc ttaaaggcca ccgcgcccgc aacagcaaac
    17221 tgtgtctgga aatggggctt ggtcaggaaa agcgccgcga cgacggcatt cctggcatta
    17281 ctaactatat cttttcagaa actgccgctc gtggaatgta ccaacgctgg gccgatcttc
    17341 tgagtagcga aagctggcag gaagtgctcg ataaaaccgc cgcttaccag caggaggtga
    17401 tgaaatgagt gcgcaagttt cactggagtt acatcaccgc attagccagt ttctctttca
    17461 cgaagccagt ctgctggatg actggaaatt tcgtgactgg ttggcacagc tcgacgaaga
    17521 gattcgttac accatgcgca ccacggttaa cgcgcaaaca cgcgaccgtc gcaaaggcgt
    17581 ccagccgccg acgacctgga ttttcaatga caccaaagac cagctggagc ggcgaatcgc
    17641 ccgtctggaa acgggcatgg cctgggcaga agagccgccg tcacgcactc gtcacttaat
    17701 cagcaactgc caggtaagcg aaaccgacat cccaaacgta tttgccgtga gggtgaatta
    17761 tctactttat cgggcacaaa aagagcgcga tgaaacattc tatgttggaa cgcgtttcga
    17821 caaagttcgc cgtctggaag atgacaactg gcgcttgctg gaacgggata tcgtcctgga
    17881 tcaagcggtg atcacttccc ataacctgag tgtactgttc tgatgaatcg aatttatgcg
    17941 tgtcccgttg cggatgtgcc ggagggtgag gctctccgga tagatacctc gcccgtcatc
    18001 gccttgttca acgtgggcgg cgagttttat gccattaacg atcgttgcag ccatggtaat
    18061 gcgtcgatgt cagaagggta tctggaagat gacgccacgg tggagtgccc gctacacgcc
    18121 gccagttttt gcctgaaaac ggggaaagcg ttatgcctgc ccgccaccga tccgctcacc
    18181 acttatccag tacacgttga aggtggtgac attttcatcg acttaccgga ggcgcagcca
    18241 tgagcgatct gcataacgag tccattttta ttaccggcgg cggatcggga ttagggctgg
    18301 cgctggtcga gcgatttatc gaagaaggcg cgcaagttgc cacgctggaa ctgtcggcgg
    18361 caaaagtcgc cagtctgcgt cagcgatttg gcgaacatat tctggcggtg gaaggtaacg
    18421 tgacctgtta tgccgattat caacgcgcgg tcgatcagat cctgactcgt tccggcaagc
    18481 tggattgttt tatcggcaat gcaggcatct gggatcacaa tgcctcactg gttaatactc
    18541 ccgcagagac gctcgaaacc ggcttccacg agctgtttaa cgtcaacgta ctcggttacc
    18601 tgctgggcgc aaaagcctgc gctccggcgt taatcgccag tgaaggcagc atgattttca
    18661 cactgtcaaa tgccgcctgg tatcctggcg gcggtggccc gctgtacacc accagtaaac
    18721 atgccgcaac cggacttatt cgccaactgg cttatgaact ggcaccgaaa gtgcgggtga
    18781 atggcgtcgg cccgtgtggt atggccagcg acctgcgcgg cccacaggcg ctcgggcaaa
    18841 gtgaaacctc gataatgcag tctctgacgc cggagaaaat tgccgccatt ttaccgctgc
    18901 aatttttccc gcaaccggcg gattttacgg ggccgtatgt gatgttggca tcgcggcgca
    18961 ataatcgcgc attaagcggt gtgatgatca acgctgatgc gggtttagcg attcgcggca
    19021 ttcgccacgt agcggctggg ctggatcttt aaggaagcac gatgaaagaa aaaacgatca
    19081 ttattgtcgg tggcgggcaa gcggcggcaa tggctgcggc ctcgctacgc cagcaagggt
    19141 tcaccggtga gctgcatctg ttttccgatg agcgacatct tccttatgaa cgacctccac
    19201 tctcgaaatc catgttgctg gaagattccc cgcagttaca gcaggtgtta cccgctaact
    19261 ggtggcagga aaacaatgtt catctgcatt ccggtgtaac catcaaaacg ctgggtcgcg
    19321 acacacgaga gttagtgtta accaacggcg aaagctggca ctgggatcag ctttttatag
    19381 caaccggcgc ggcagctcga ccgctgccgt tgcttgatgc actgggagaa cgctgcttta
    19441 ccctacacca tgccggtgat gccgccagac tgcgagaagt tctgcagccc gaacggtcag
    19501 tcgtgattgt cggtgccgga actattggtc tggaactggc tgccagcgcc acgcagcgca
    19561 gcgcagatgt aaggtgacag tgattgaact ggcggcaacc gtcatgggcc gtaatgcacc
    19621 accgcccgtg caacgctatc ttttacagcg ccatcagcag gctggtgtgc gcattctgct
    19681 caataatgcc attgaacatg tggtcgatgg tgaaaaagta gaactgacgc tgcaaagtgg
    19741 ggaaacgctt caggctgatg tggtgattta cggtattggt atcagcgcca acgagcaact
    19801 ggctcgcgag gccaaccttg atactgccaa tggcattgtc attgatgagg cttgccgcac
    19861 ctgcgatccc gcgatctttg ccggtggcga tgtggcaatc actcgtcttg ataatggtgc
    19921 actacaccgc tgcgaaagct gggaaaacgc caataaccag gcgcaaattg ccgctgccgc
    19981 aatgttaggg ctaccgctac cgctactgcc gccgccgtgg ttctggagcg atcagtacag
    20041 tgataactta cagtttattg gcgatatgcg tggcgatgac tggctttgtc gtggcaaccc
    20101 ggaaactcag aaggcgattt ggtttaatct gcaaaacggc gtgcttatcg gtgcggtcac
    20161 gctgaatcag ggccgtgaga ttcgcccaat tcgcaaatgg atccagagcg gcaaaacgtt
    20221 tgatgcgaaa ctgctgatag atgagaacat cgcgcttaaa tcactgtaat caggataatt
    20281 agcgaatatc tcaatgcctg gggcgtggcg aggtgcaaga gtgtgtatta cgtttaaatc
    20341 acattatctt gcaaagggat tggttatgaa cacactacgt tattttgatt ttggagctgc
    20401 ccgccccgtt ttgttattaa ttgcccgtat cgccgtggtc ttaattttca ttatttttgg
    20461 ttttcccaaa atgatgggct ttgacggtac ggtccaatat atggcctcgt tgggcgcgcc
    20521 aatgccgatg ctggcagcga ttattgcggt agttatggaa gtgcccgccg cgatattaat
    20581 cgtgcttggc tttttcaccc gtccgctggc ggtgctgttt attttctaca cgctgggtac
    20641 ggcggtgatt ggtcaccatt actgggatat gaccggcgat gcggttgggc caaatatgat
    20701 taatttctgg aagaatgtca gtatcgctgg cgcgttcttg ctattggcaa ttaccggacc
    20761 gggggcaatt tctctcgatc ggcgttagga aagatgccgg atgcggcgtg aacgccttat
    20821 ccggcatttt ataaattaca gccactctac ccgcaacgac atctctgagg ttgttgattc
    20881 ccctggtgct agcgcaatga gatcaccccc ttccgggcga tgatgctcat ccggcgcatg
    20941 gctcatcggt tccagacaga agaaatcaaa cgcatatcct ttatcaaacg cagggtcgga
    21001 aacaaagatg aaataacacg gtgcaggtgg cgtcgtttcc atgatgatgg cataaccttc
    21061 ctgcggctgc tcgatgcggg cctgaccatt ccatccggca aaaccattgt tcacccactg
    21121 acgcggcaac ggcgcgagct ggttaaaatc cagttcctgc ggtagctgct cgcaaaactc
    21181 acccgccagc cactgctccc gttccagcca gtaaccgctc gcctgcgcct gaatccgcgt
    21241 ttgtggtgac aacggaaaat aaggatgcca gccggtacca aatggcagcg tctctgcccc
    21301 ttgattggtg acagagagcg tcaccgtcag cgtatccgcc gttaaatgaa acgcctgact
    21361 tacccgatag tgatagacac cgctgcgatg ttcatacacc agacacaaac tatcatcgct
    21421 gtgcgagaca cattgccact cgcccagcca gccatcgccg tgtagatagt gcgcatccca
    21481 ctcaacattc ggttgcagtt gatactcacg cccctgccag acaaaacggt tgccgctcac
    21541 ccggtttgca aatggcacca gcggaaaaca tgaggcatcg gttgccacac cgcttttttt
    21601 accaggacgt aagagcggcg tcgtatcgcg ccagaagcct tcgattacgc cgccctggtc
    21661 agaaacgtcc agctttagcg acccgtggga taaggtatag atggtcatcc gctaactcct
    21721 taatccggga agttaatcac aactttcccg catttgccgc tcgccatcag cacataggca
    21781 tcacctgcct gttctagcga gaagcgatgg gtaatggcgt tacgcggcca cagcttccag
    21841 tcagtcaaat cgtgggcgca tttttccata tggaacagac tggtcaccca ggagccgata
    21901 atccgccgtt gatggtgcat cagatcagcg ctgacctcga attccacttt tccggtttca
    21961 ccaatgtaaa ccacccgccc ccagtcagca gtggattgca gtgccagcaa gcgacctgcg
    22021 gcattaccgg aacaatcgag cgcaacatcc gcgccaccgt gggtaagttc agcaattatc
    22081 tgcggcagac cttcggtggt tgctaaatag ccgtgatcca tcccccctaa ctgttttgcc
    22141 atcgccagac gttccggcag catatcaacg ccgatgatcc gttttgcacc gcgacctttc
    22201 gccagcatca tcgccatcat gccgaccggc cccagaccga ccaccagcac gttatcgctg
    22261 ccggaaactt cgccgcgcaa aatcccttca taggccgtgc caacgccgca actgataaac
    22321 gcaccatctt cgtagctcag cgcatccggc aggaggatca gatctttttc ttccgccagc
    22381 agatattcgg catgaccgcc gtcacgctgc cagccgtaag ccgcttttcc tttgccagta
    22441 caagagatag gaaagccgcg gcggcagttc gggcaaaaac cacagccaga aatgtgatac
    22501 accagcacgc ggtcgccctc tttaaaatgg cggcagcctt gccccatcgc cacaatctgc
    22561 ccgcacggtt catgaccgtt gataaatccc tggtataacg gtttatcggg tgccgccgcc
    22621 gtggcacggt gttgatgata gatatagtgg acatcgcttc cgcaaatccc ggaggatttc
    22681 attttgatca gtacctggtt aatccccggc gtcggcaccg caacttcccg cagatcgacg
    22741 gttgaatttc ctagtaaata agcagccagc atcgttttca taaatccctc attaacaata
    22801 cgattaattt tcatccctgc agcacccgcg ccaggggcgt taaggtttag cgtttcgctt
    22861 tactgcttcg ctgggtcagc aagatattcg ccagcaccgc caccacgatg atgacgccgc
    22921 gtaccacctg ctggaaaaag gagttaatac cgagcagcac cagaccgtta ccgattagcg
    22981 taatcaccag cacaccaagc aatgtaccga acagggaacc gcgaccgccg gaaagtgccg
    23041 taccgccgac cacgaccgcg gcgatgacgt caaactccag accgtttgcg gcacctgcgt
    23101 taccggaacc gaggcgtgcc gccaacaaaa tgccggtcac cgccgctaat aatcccgaaa
    23161 gggtaaagat aagaatgcgc actcgacgaa cgttaatacc gcacaactgc gccgccgtgg
    23221 cattaccgcc aacggcaaaa accgagcgcc cgaaggcggt ttttcggcta atgaacacaa
    23281 acagcgcaaa caacaccatc atgatcagcg cggatactgg cacaccgaga aattgtccgc
    23341 ccagccagtc cagcacctcg ttttcgtcaa tcggcactgg cagtgcgttg gtcataaaca
    23401 gccccattcc gcgcagagcg ctccacagcc ccagtgtggc aacgaaactt ggtacgttaa
    23461 acacgccgcg cagcaccccg gcgagcgttc ccatcagcgc gcctaacagc aacaccagca
    23521 gacaagcaat cgccaacgga acttcaaatt gcagcaaaaa tgccaggcac accgagacaa
    23581 aagccaccat cggcccaacg ctgacatcaa tttcaccgga gataataatc agcgtcatcg
    23641 cccaggcggc aatcccaatg gtggcggcat cgcgcagcac gttcatctgg ttattcaatg
    23701 agataaagcc aggcgcgttc agggagaaga ccagataaag aatggcaatc accaccagca
    23761 aaccgatctc attaatatgg cgactgacaa attgtttgag cgagacgctc ttgccctgcg
    23821 gcaatggtaa tgacgaagca tacatcttca ggttcctcgg taaaattcat cagtgcacag
    23881 acagaatggc ggacatcagc tcatccacat tgaccggcga gtgaaactcc tgcgagaacg
    23941 tgccgtgctg taataacagg atgcggtcac acaccagcgg caattcctcc acttcactgg
    24001 agataaacac cacgcttttt ccttcggcag ccagctcacg gacaatacgg taaatctgct
    24061 gtttggcttc gatatcgacg ccgcgcgttg gctcgtcgag caacaaaatc tggctggcgg
    24121 catagaccca acgaccgatc accacttttt gctgattgcc accagaaagc gtgccgatgg
    24181 gtgtttcgct actggcagcc ttgaccgtca tccgctgcat cacctcttcg gtcaggcggc
    24241 ggatggtgga ccattgcagc acaccgttgg tgctgatttt ttgccgattg gtcagcactg
    24301 tattttcgtc aacgcctagc cagggaatga tccccgcttc tttgcggttt tctggcgtat
    24361 agccaatgcc gcgtttcagc atgtcgccgt aatcggggcg catgattttc tcgccgttga
    24421 taacaatttc gccctgttca tacgtctcca gcccaacaat cgccttcagc aattcactgc
    24481 gccctgcccc cagcaggcca gcaatgccaa gcacttcgcc acgacgtagc gtaaagctga
    24541 tatcctccag cttgggctta tggcgtaacg cacggacttc cagtacggcc tgatccacaa
    24601 tttcctgagg ggcaaccggc gcaatatcaa cgtgatcacg ccctagcatt agcgacacaa
    24661 tatgatgcgt ggaggtgttt tcgagcatca catcgcccgc cacctgacca tcgcgcataa
    24721 cggtggcata ggaggcaatg cggcggattt cttccatccg gtggctgaca taaatcaccg
    24781 ccacgcccag tgccgacatc tttttcaccg cactgatcac cagttcaact tctgcactgg
    24841 caagcgagct ggtgggttca tcaagaatga ccacgcgcgg ctcgcccttc atcacccgtg
    24901 caatttccac cagttgcttt tgcgccgggc ttagcgttga aacaagttgt tcaggactaa
    24961 cgtcaacgcc cagcgcctgt aagcaacgtt gggcatcctg cgccatttgc aggtaatcaa
    25021 tcatgccgtt gcggcgaggc cattgaccaa ggcagaggtt ttctgccacc gtcagccctt
    25081 ccaccagact taattcctga taaaccgcac gaacccccag ttcagcggca cggcgagtca
    25141 acgtagcttc gtcaccttcc agtcgcgtct cgccaatcca gatatcaccg ctatccgggc
    25201 gttcgctgcc ggtaagcatt cgaatgagag tcgatttgcc cgcgccgttt ttacctaaca
    25261 gcgcacgaac ttcgccttta ttgagcgtga agttaacgtt atccagcgca acgacgccgg
    25321 ggtaacgctt atttcctgcc accacttttg ctaccgggac tgcctctgtt gccgtgaaca
    25381 tagggacctc tgcgaatcag cgattagggc agaccatcaa cgtgcgttgc cagccactgt
    25441 ttaccatctt ccgttttggt atagagatcg attggcacct gaatcacttt ttcaccgtcg
    25501 gcttgtttat tgataacctt caatgtttgc gcgaaaacag cattgcccat tttcttaccg
    25561 gaaatatcca ctaccgcttt cagcacctga ttgttttcca gctcctgagc aatttcggtt
    25621 gtcatatccg aaccgaaaac agcaattttt ccggcctgat tttgattacg taccgctttt
    25681 accgcgccga gtgtcgcacc gcccgactcc cccataatgg cgttgagatc cggcgtggag
    25741 ataatcagtt tttcaccaac ggaaatcgct ttatctaaaa cagtcccttc ctggttagcg
    25801 acaatttgcg cgccgggaac gcgggatttt aatacttctt caaatccttt acgtcgctgc
    25861 acacaaactt caaaggcttc gcaattgatg acggcaattt tcggctggtc aattttattg
    25921 gcaataaaat aatcggcggc agcgttaccc agtttttacc aaattccagt ggatcgccga
    25981 ccagatacgc cgagacatat ttatcgacac ccttttgatt aatacaggtg ttgtagcaaa
    26041 tcaccggaat acccgcttca ctggcgcgac gaacggtacg gctactgcca ttttcagaca
    26101 ctgccgataa aataatggca tcgacattac gcgccacgag ggtatcaaca aaggtacttt
    26161 ctttcgaaat atccccctgg gcgttagttt caattaactg tacctgtact gaagaatctt
    26221 ttgccgcatc ctgaacgccc tgacgcactc cagcgtaata tccctgggta tcaaggtata
    26281 ttgcaccaat ggtcatttct ttatccgcag ccctggcaaa tagtgcgcta cctaatagcg
    26341 tagccattaa taatagatta cgggttgttc tcattttttt aggcatagtg cctcctgtag
    26401 ggtttttatt aacaacggct tattctaatt attttgtgat gagcggcagc gcgtgccgct
    26461 caaatattac agttaatagg gtttagtgaa cgtagttaaa aataaaaggc atcacagtgg
    26521 tgaataatgc cgctttcggc caggccggat taatcgccgt caattcagcg cgagcagact
    26581 catattgcgc aacctccccg agtcccgcgg acgccagcat cgccaccatc aggcattttt
    26641 ctttatgctg ctgttgtaaa tcgccataca gcgataacag gtcaggttgt gaaacagcaa
    26701 agaaatccgc ctcaatactg gttttcgcca tctcttgcgc ccactgtttc atttcactaa
    26761 acagttgctg tgcggtttgc tgttcaccca gcagtcgcag cgccattcct tgccagaaga
    26821 gataatcaac cggctgatcg ttgtaataac tgtggatgtt aatggtgcga tcgccggtcg
    26881 ccgccagacg taaacaacgc atcgcttcag tttcatcgcc ctgcgcgttg gcgcatatcg
    26941 cctgccagaa ccagatgtcg ttatcagttt gccccggtaa acggccttcg cttaaattct
    27001 ccggataata cagcgcggca tgaagcagtt cgctggcctg ctgcggctct ctggcatcaa
    27061 gatgctgcca ggcgcgtaat aactggttga ggataaactg actggtgacc ttcccttccc
    27121 cgccttccca cgggtggaat ttacgcgtgg cgagaatgtc cgccgctttg tctgcctgac
    27181 ccgtgagatg ccacaaattg agcagttctg cggtcatgtc gtcgcgtttc agcgcaattt
    27241 ccagattatt ttccagacgc gccagtcgtt tctccggtgt ggctccactt aacttatcca
    27301 gcaaatcccg ttcgaaaagc agacgtgcat cctgcggcgc aagctgataa gcattatcaa
    27361 gataacgcgc ggccagctca taatcgtgtt tcttattcca cgcatggatc gctaacccgc
    27421 gccagccgtc ggcaaactcc ggcgacatct ctacgcaacg ttgccataag gcaatggctt
    27481 tgttgtagct acgtttgttg tagtagaagc aggccagtaa atggcgagca aaccaacact
    27541 cttcaatact ctccagcgcc gccacttctt ccagcgtatt cgggaaacgg acaaactgcg
    27601 ggaagacatc aatggctttt gcgaccagtt cgccacgttc ggctttcggc agcaagctgg
    27661 cttgcaggta aagcggcagc gtgcgctggc agtccagtgc gttcagcatc tctgccgcca
    27721 gggtgggcat tccccagtta atcagttgcc cggcggtcat cagagcgtta acgtcgcgtc
    27781 cctgacacag accgcgccac tgcgcgaggg ctgattcgct acgaccatcg aaccagttca
    27841 gccaccacag agtggcgttc agcggataat cacgcagcag tttctcgcgc tgcaaacgcg
    27901 cgttgtcctg acgaccactt aacaccagca gcagattatg caagcaaagc acttcctgat
    27961 tggttgggca ggcacgaaga ctttgttggc aaaaatccag acccgcgtcg aagttatcat
    28021 tacgcgccgc cagtcgtgcc agaccataat agccgcctgc tttactattg ccgctccaga
    28081 ccgcacgcca gaaatcctct tcggcttgtt gatattgtcc ctgacgttcg taagcactgg
    28141 cgcgaatcaa acttgcttgt ccacactgcg gttttttgtt cagcgcatgt gcacgtttca
    28201 gcgcctgact ggcatacgcc accgcttgcg ggaaatctgc gcggttatat tccagcatcg
    28261 ccagcgccaa gttacagcga taatccagcg ggtccagcgc cacgccgcgc aggtagtaat
    28321 cgaacggtga acggctggcg tgatgatatt gctccagatg ctgaccgata aaccaggctt
    28381 catctgtact ggtaatgtct tgtgctgcca gtggcgcttt ggcgacgtcc ggcaacggca
    28441 actcctgcgg ctgatgttcc tgataactca gtacaatatt gccgtcggca tcggagagtt
    28501 caatggtcag tctgtcagga ttgataccgt gcaacacgcc ctggatggcg gtcgctggtg
    28561 tcagggccac ggcatcatcg agcaacgcgt tgcatttgcc gatttcgcgg atcgccagac
    28621 ggtatccgtt caacggagag atggcataca gcccccactc aatcccccgc tcactacgct
    28681 ggagttttat caccgcatcg cgggaggcat tttgcaccat gcccagagaa tgataaggca
    28741 ggaaatactg ctcgaaacgc ttctcttcgt aagcatcaag ccaggtaaaa tcaggctggt
    28801 tatcggcaaa aataccggtc atcagttcga tatacgggcc gttattgtcg gtcagactct
    28861 tatcccacgc ctggccaaat tcactgtgtc cccaactcca ctgtttttta cctggcgcaa
    28921 tatggtggtt ggcaacgtgc agcaaaccgc catcttcatc gtgacaccac gcgccgacaa
    28981 aatcgtactg cgatttttcg gccatatatg aggttggaac aggcacattt ttatagcgag
    29041 aaatgtccac tccggcggag tagtccactt tgtagtaagt gccggtggcg atggggaaag
    29101 cggagacggc ccgtttgccg tgatcaaaca ccgcagtcac atccggtggg aaaacgctct
    29161 gatgcccttc accccctttc actgccgggt tgacccacca caagaaatga cgcggcgtgg
    29221 cgttgccgtt atagacgcgg ctggcgattt ccagtgccgc ccggtcaggg cgcagggtga
    29281 aacctgtcat cacctgtaaa ccatgcatcg gctccgtttc gccgacccac accgtctgtg
    29341 caccgtcttc atgggcttcg agggtgaaat caacgggcat aaaggtggtc gggcgatggt
    29401 gttgcggcca gttaaactca atcccgccgg agatccacgg tcccagcatc cccaccagcg
    29461 caggtttaat gacttcattg tgatagacaa aatcgcgctg tttcacttta tcccatgcgc
    29521 gatgcacccg accgcccagt tccggcagga tcatcacttt gatgtagtcg ttttccagcc
    29581 acaccgcctg ctaggatttc agggttttct gctcgctcag cgtatcggtc acgccgtagg
    29641 gataaaccgc gccggacgat ccctgataaa cgcgattttc caggaacatg ggatgtatat
    29701 cctgcggccc ggtttcatag gtcgggatct caacgcgctc ttgccacact tttactggag
    29761 tcatggtgcc ctcaatatta acaagacata ctgaattaaa agatttgtgg cagtgtattg
    29821 aacaatctgg caatgttttc gcggaataat cacgcaatta actaaacaag gtttagtgaa
    29881 gatgagagcc tgcattaaca atcaacagat tcgccaccat aacaaatgcg tgattctgga
    29941 actgctgtac cggcaaaagc gcgccaataa atcaacgctg gcccggctgg cgcaaatttc
    30001 gattccggca gtcagtaata ttttgcagga actggaaagc gaaaaacggg tggtgaatat
    30061 tgacgatgaa agccagacgc gcgggcatag tagcggtaca tggctgattg cgccggaagg
    30121 tgactggacg ctgtgcctga acgtgacgcc caccagtatt gagtgtcagg ttgctaatgc
    30181 ttgtttaagt ccgaaaggcg aatttgagta tttacagatt gatgtaccga cgccgcaggc
    30241 gctgctgtcc gaaatcgaaa aatgctggca tcgccaccgt aaattgtggc cggaccgcac
    30301 catcaatctg gcgctggcaa tccacgggca ggttgatccg gtgaccggcg tatcgcaaac
    30361 catgccgcaa gcgccgtgga caacgccagt tgaggtgaag tatctgctgg aagagaagct
    30421 gggcattcgg gtgctggtcg ataatgactg cgtgatgctg gcgctggcgg agaaatggca
    30481 aaataattcg caggaacggg atttctgcgt gatcaacgtt gattacggca ttggctcgtc
    30541 gttcgtgatt aacgagcaaa tttatcgcgg cagtttgtat ggtagcggac agattggtca
    30601 caccatcgtt aatccggatg gagtcgtctg cgactgtgga cgttacggca gcctggaaac
    30661 cgtcgcctcg ttaagtgcat taaaaaaaca ggcgcgggta tggctaaaat cacaaccggt
    30721 taatactcag cttgagcctg aaaaactgac aaccgcgcag ttaatcgctg cctggcagag
    30781 tggagaaccg tggatcacca gctgggttga tcgctctgcc aatgccattg gtttgagtct
    30841 gtataacttc ctcaacatcc tcaatattaa tcagatttgg ttgtacggtc gcagttgtgc
    30901 ctttggtgag aactggctta atactattat tcgccagaca ggatttaacc cgttcgaccg
    30961 cgacgaagga ccgagcgtga aagcgacgca aattggcttc gggcaattaa gccgcgcaca
    31021 acaggtgctg ggaattggct atttgtatgt tgaggcgcag ttacgacaga tttgatggcg
    31081 cgataacgta gaaaggcttc ccgaaggaag ccttgatgat cataaacgaa aaattgcctg
    31141 atgcgctacg cttatcaggc ctacacggag attgcaatat attgaatttg caagattttg
    31201 taggcctgag aaggtgttca tgccgcatcc aacatgaaca acgagcacat tgtcagcaaa
    31261 tcaccgtttc gcttatgcgt aaaccgggta acgtgcgcag atgtcgagaa ctttaccttt
    31321 gatgcgctcg ataacggctt catcattgat gctgtccagc acgtcacaca tccagccagc
    31381 cagttctttc gcttcggctt ctttaaagcc gcgacgggta atcgccggag tacctacacg
    31441 aataccggag gtcacaaacg ggctcttcgg atcgttcggt acgctgtttt tgttgacggt
    31501 gatgttagca cggcccagag cggcgtctgc ttctttaccg gtcaggtttt tatcaaccag
    31561 atcaaccagg aacaggtggt tatcagtgcc gccggaaacc actttgtagc cgcgctcgag
    31621 gaacacttct accatcgctt tagcgttttt cgcgacctgc tgctggtaag ttttgaactc
    31681 aggctccatc gcttctttca gagcaaccgc tttaccggcg attacgtgca tcaacggacc
    31741 gccctgacca ccagggaaaa cggcagagtt cagttttttg tacagctctt cgctaccacc
    31801 tttcgccaag atcaggccgc cgcgcggacc cgccagggtt ttgtgagtgg tggtagtaac
    31861 aacgtgagca tgaggaaccg ggttcgggta gacgccagca gcaaccaggc ccgcaacgtg
    31921 cgccatatca acgaacagat aagcaccgat gctgtcagcg atttcacgca ttttcgccca
    31981 gtcaaccacg ccggaatatg cagagaaacc accgataatc attttcggct tgtgttcttt
    32041 ggcttgtttt tccagatcgg cgtaatcgat atgaccggta gcatcgatac cgtaaggaac
    32101 gatgttgtac agtttaccgg agaagttaac cggagaaccg tgagtcaggt gaccgccatg
    32161 cgccaggttc atacccagaa cggtatcacc tggttccagc agcgcggtgt agaccgcaaa
    32221 gttagcctgg gagccggagt gcggctggac gttagcgtag tcagcgccga acagttcttt
    32281 cgcacgatcg atcgccagtt gttcaacgat atcaacatac tcgcaaccgc cgtagtagcg
    32341 tttgcccgga taaccttcag catatttgtt ggtcagctga gaaccctgcg cctgcattac
    32401 gcgcgggctg gtgtagtttt cggaggcgat cagttcgatg tgctcttcct gacgtacttt
    32461 ttcctgctcc atagcctgcc acagttcggc atcataatcg gcaatgttca tttcacgctt
    32521 taacatccgc atctcctgac tcagctaaca ataaaatttt tggcctttat aggcggtcct
    32581 gttggacaac ggcgaacagt ataaccgaat cattgtgcga taacaggtct tgacaaagga
    32641 atttacgcaa acgattacct tcaggctacg caaggctttg gagaataaag agcttgcaac
    32701 cggaaacgga tttcttttca ggtttgtgat gcaaattttt cacttcatca cattctttct
    32761 gaaaaacacc aaagaaccat ttacattgca gggctatttt ttataagatg catttgagat
    32821 acatcaatta agatgcaaaa aaggaagacc atatgcttga cgctcaaacc atcgctacag
    32881 taaaagccac catcccttta ctggtggaaa cggggccaaa gttaaccgcc catttctacg
    32941 accgtatgtt tactcataac ccagaactca aagaaatttt taacatgagt aaccagcgta
    33001 atggcgatca acgtgaagcc ctgtttaacg ctattgccgc ctacgccagt aatattgaaa
    33061 acctgcctgc gctgctgcca gcggtagaaa aaatcgcgca gaagcacacc agcttccaga
    33121 tcaaaccgga acagtacaac atcgtcggtg aacacctgtt ggcaacgctg gacgaaatgt
    33181 tcagcccggg gcaggaagtg ctggacgcgt ggggtaaagc ctatggtgta ctggctaatg
    33241 tatttatcaa tcgcgaggcg gaaatctata acgaaaacgc cagcaaagcc ggtggttggg
    33301 aaggtactcg cgatttccgc attgtggcta aaacaccgcg cagcgcgctt atcaccagct
    33361 tcgaactgga gccggtcgac ggtggcgcag tggcagaata ccgtccgggg caatatctcg
    33421 gcgtctggct gaagccggaa ggtttcccgc atcaggaaat tcgtcagtac tctttgactc
    33481 gcaaaccgga tggcaaaggc tatcgtatcg cggtgaaacg cgaagagggt gggcaggtat
    33541 ccaactggtt gcacaatcac gccaatgttg gcgatgtcgt gaaactggtc gctccggcag
    33601 gtgatttctt tatggctgtc gcagatgaca caccagtgac gttaatctct accggtgttg
    33661 gtcaaacgcc aatgctggca atgctcgaca cgctggcaaa agcaggccac acagcacaag
    33721 tgaactggtt ccatgcggca gaaaatggcg aagttcacgc ctttgctgat gaagttaagg
    33781 aactggggca gtcactgccg cgctttaccg cgcacacctg gtatcgtcag ccgagcgaag
    33841 ccgatcgcgc taaaggtcag tttgatagcg aaggtctgat ggatttgagc aaactggaag
    33901 gtgcgttcag cgatccgaca atgcagttct atctctgcgg cccggttggc ttcatgcagt
    33961 ttaccgcgaa acagttagtg gatctgggcg tgaagcagga aaacattcat tacgaatgct
    34021 ttggcccgca taaggtgctg taatttgatg ttgccggatg gaaacatccg gcaacccttg
    34081 acgcggttta aattgccgcg tcgtcctctt caccggtacg gatgcgaatg acccgagcca
    34141 cgtcaaagac gaagatttta ccgtcaccga ttttgccggt ttgcgccgtg cgaataatgg
    34201 tatcgacaca ggtatcgaca atgtcgtccg gtacgacaat ctcaattttc actttcggca
    34261 gaaaatccac catatactcc gcgccgcggt acagctcggt atggcctttc tggcgaccaa
    34321 agcctttcac ttcggtcacc gtcatgccgg taataccgac ttcggccagt gcttcgcgga
    34381 catcgtccag cttgaagggt tttataatcg catcaatctt tttcatgcta ttccttgaaa
    34441 aggttgcctg tcttttgatc tgctaaacgt aacacataac gccaattcat tccttgaaat
    34501 cgtttgcatc cagctcgtgt cgggaaagca gtttataaaa ttctgtccgg ttgcgccccg
    34561 ccattctcgc cgcgtgggtg acgttgcctt tggtgatttg cagcagctta cgcaagtagt
    34621 tgagttcaaa atgattacgt gcctcaacaa aggttggcag cgccgtattt tcaccctcca
    34681 gcgcctgctc caccagcgca tcactaatca ccggagatga ggtcagcgcc acgcactgtt
    34741 caatcacgtt gaccaactgg cgcacattac ccggccagct cgcggtcatc aggcgtttca
    34801 tcgcatcggt agagaacgcg cggacaaacg gtttatgtcg ctctgccgcc tggcgcaaca
    34861 ggtgatttgc cagtagcgga atgtcttctg tgcgctccgc cagtgccgga attttcaggc
    34921 tgacaacgtt gaggcggtaa tagagatctt cgcggaattc cccgcgcgcc atcgcttttg
    34981 acagatcacg gtgagtggca gaaataatcc gcacattgat atcaatatcg cggttactgc
    35041 ccagcgggcg cactttacgc tcctgcaaca cgcgcagcag tttgacctgt aacggcgcag
    35101 gcatatcgcc aatttcatcg agaaatagcg ttccgccttc cgccgcctgg aataaacctt
    35161 cgcgattgct gacagcgcca gtaaacgcgc cacgcgcatg accaaacagc tccgactcca
    35221 gcaattgctc gggcaatgcg ccacagttaa tagcaataaa tggtttgctg ttgcgcgggc
    35281 tggcgttgtg gatagcctgg gcgaaaatct ctttcccggt gccgctctga ccgttaatca
    35341 aaacgctgac gtctgattgc gccaccagcc gcgcctgttc cagcaaacgc agcatcagcg
    35401 ggctgcgggt gacaattgcc tcgcgccagc gttcatcggt ggctggcgcg gattgctcca
    35461 gcgcatcgtc aattgcctga tatagcgcat ctttatcgac aggcttggtg aggaaactaa
    35521 aaacgccctg ctgtgttgca gcaacggcat cggggataga accatgcgca gtaagaataa
    35581 ttactggcat tcccggctgc actttctgga tttcagcaaa tagctgcata ccgtccattt
    35641 catccatccg caggtcgctg ataactaaat ctactttttc gcgattcagt acccgtaatc
    35701 cttcagcgcc actttccgcc gtgaccacac tgtagccttc gctggtcagg cgcaggccaa
    35761 gcagtttcag caatcccgga tcgtcatcga ccaataataa atgcgcaggt ttatggctca
    35821 tcaggagtga cctcatgggt ggatggcgcg ggcttttcac tctcgtgcgg cgtatccggc
    35881 gagaaatttc cagccggttt gcgggtcgag agctggcgtt caatatcggt caggttttcc
    35941 agcttgcggg tggtgagttc cagttgctgc tgtagaacgt gatgttgctg gcgcaatgta
    36001 tccagctcgc tgtcgctgga ctgctggagt ttactgtaac gttggcgctc ttccgccagt
    36061 tgcagttgca gcgcctgacc gtcgcgccag agttgataca gtgggcgaac ctgtgccggg
    36121 atctcggtac ttaacgcttc aatacgcgcg accagctggc ggcgctcata cggcgtaatt
    36181 ttggcgtcgg cgagcaaaat cccttgttta aaggtatttt gccagctgcc gtcgtcatat
    36241 tggcgagctt gctgacgcga ctgcgcaggc attaaacgat cagcacaatc catcgcccgc
    36301 agccagtaaa gcggattggt ttcggttgat ttaccttgca gcgcccagat gtcgctacat
    36361 tcagtagaaa gatagtcagc cagttgataa accggaattt tttcttctgc tggcgtatca
    36421 atggctggct tattgtgatt ctgcacgcaa cccagcaatg ccagacatgg cagccctgcc
    36481 agccacagcc gtcggggcaa taatcgttga aaaatgtgtc gcatattcac cagacttaaa
    36541 gcctatccca gtgggcgtaa ttgttgcaga cagtctggac atggacagcg cggagaaacc
    36601 ggggcgtaca tatcgtacgt gaagatttcg agcactaccc ggggccgaac tgacaaataa
    36661 aatagcctga taggataggc tgttagcatt atttcgtgtt tttcgacgac ggtaattcaa
    36721 tgcggaaaca aacgtcttgc ccgctctcgt cgaccagata cagttcccct tgcatacggc
    36781 gaatacaatc cctggcaatg cttaatccca gaccgctgcc cttcaccgcc ccttttcgct
    36841 ggtggcttcc ctgaaaaaag ggttcgaaga tcatggcgcg ttcctcttgc ggaatgggcg
    36901 tgcctgtatt gatgacatca atataaaccc gcgcaccatg taaactgctg cgaaggcaaa
    36961 tgttaccgga ttcagccccg tagtgcaccg cattggagta aagattatcc agtacgctca
    37021 tcagcagcat tggctccgcc aggcaagctg ttgctttgag atcgacgtcg gtatgcatca
    37081 ttttagcccg tgcgggcagg ctatgagcag aaaccactgt ctccaccagc ggtgctaatt
    37141 caacattctc cagttccacc gcactgtccg cctgtttacg gttgtaatca agcagttgtt
    37201 cgatcagttt ttgcaaattg cagctgctgc tatcaagaat gctcaccacc tctttttgct
    37261 ctggcgtaag cggcccgaca acctgatcag ccagtaattc agtgccctcg cgcatactcg
    37321 ccagtggcgt ttttaattca tgagataaat gtcttaaaaa ttgatggcgt tgggattcca
    37381 gccatgacag gcgctcactt aaccaaagaa tacgttgccc aaccgagcgt aactcgctcg
    37441 gtccacagaa cgaaacgcta ttgcccagag aacgcccttc ccccagacgg ttgatcatgc
    37501 gctcgatatt tttcaccggc ccgataatca tccgcgtgaa aagtagcacc atcaccagac
    37561 tcaccagaaa tagcaccagc gattgccaac caaaatattg cccacgttcg gcgatttcac
    37621 gctgaagttg ctgcccacga gagaacacca ctgtgcgcgt ggcctgcacc atttcggtat
    37681 tggcactggc aaaggcttca agacgcgcgg cggcggcagc atcgggacca ctgttgttac
    37741 actgaagttg ggccagattg ttcaaatcct gacgcaatgc ctggtaaagt ttatcgtctg
    37801 gcagtacgcc tgcgtgggca tcgagcattt cgctgtaacg cttgcgctgg ctttgataaa
    37861 ccttcgcaag cgtagggtcg tccagcacgc aatactgacg gtagctacgc tccatctcca
    37921 gcgccgcgtt ggtcatcgct tcactacgcc gggcatcaat aagcgtagtg cggttaacca
    37981 gcgccgcctg atcgctaagc gcattcaggc tttgccaggc ttgccatgcc agcaccaaca
    38041 ggggtagtag aattagcaaa aatgccagca ttaccagttg tcgtaatgag cggggaaaaa
    38101 cgggccagcg tttcaaggtg ttactctcgt cagacgcgaa tagcctgatg ctaaccgagg
    38161 ggaagttcag atacaacaaa gccgggaatt acccggcttt gttatggaat aaggcggtgc
    38221 ctaactcgac gtttcgcccg atggttgata tagctacgct gatatcagaa gttggacggc
    38281 aggcaccttg ttgtgcgtca ttcgaatttt atgtagcacg tcccgaaggg gctgacataa
    38341 gtcggtgaat gagccactgc ttactattat gcagtaactg tgccaataaa gaaaatagtt
    38401 tggtaacgta ctgattatac gttgctttga gggatttatg gcttcttcgc tatttgaatg
    38461 taaatcagcc actgtgtcgc taaaaagaga caacttaaga taaacttatt agattacaat
    38521 aaaatcaatc atttaagtgt cgccagaaag cgacacggta aaccacccat tgtcgtgatt
    38581 tacagacaca aaaaagctcc cggagttggg agcttatgat agtggttggt gcttaatgcc
    38641 gcatccgggc tgcaaaacca atgaactgat gacttacccc aactgcttac gcgcattgcg
    38701 gaaaatgcgc atccatgggc catcctcgcc ccagttttcc ggatgccagg agttgctgac
    38761 agtacggaaa acacgttccg ggtgcggcat cataatggtg actcgaccac tttcagtcgt
    38821 aaccgccgta ataccgttcg gtgaaccgtt cgggttagcc gggtaggttt cagtgacttt
    38881 gccgaagtta tcgacatagc gcagtgccac cagccctttg ctttccagtg ccgccagatg
    38941 cgccgcatca cgcacttcca cgcgcccttc accatgagag acagcaatcg gcatttgcga
    39001 gcccaccatc ccctgcaaca gcagagacgg gctttgggtt acttcaacca ggctgaaacg
    39061 cgcttcaaag cgatcggagg tgttacgcac aaaacgtggc cacaactcac tccccgggat
    39121 cagttcacgc aggttagaca tcatctggca accgttacat acccccaggg ccagcgtttg
    39181 cggacgatgg aagaaggttg caaactcatc gcgtacacgg tcattgaaca ggatagactt
    39241 cgcccaacct tcaccggcac ccagcacatc accgtaggag aaacctccgc acgcgaccag
    39301 ggcgtgaaaa tcctccagtc ccgtacgtcc agtcagcagg tcgctcatat gcacgtcgat
    39361 agcatcaaag cccccacgtt ggaaagctgc cgccatttca acatgcgagt tcacgccctg
    39421 ctcacgcagt acggcaactt tcggacgtgc gccagtggca atatacggtg ctgccacatc
    39481 ttcgttgata tcgaacgaca gttttacatt caggcccgga tcggcgtcgt tagatttcgc
    39541 ctgatgctcc tgatcggcac actccgggtt gtcacgcagg cgctgcatct gccaggtagt
    39601 ttctgcccac cagacacgca acgtagtgcg gctttcgctg aatacagtct gcccgttggc
    39661 ggtaatcaca aaacggtcac cggaaaccgc ctgcccaaca taatggacgc aatccgcaag
    39721 cccatgctgt gccagtacgg actcgaccgc ttcacggtca gcggcacgaa cctgaatcac
    39781 cgcacccagt tcttcgttaa acaacgccgc caggcgatcg tcacccagag aggcgatatc
    39841 cgcgtcaatg ccacaatgac ctgcaaaggc catttccgcc agcgttacca gcaggcctcc
    39901 atcagagcgg tcgtgatacg ccagcagctt acgctgtgca accagcgcct gaatcgcgtc
    39961 atagaagcct ttcagttgga caacatcgcg tacatctgcc ggtttgtcgc caagctgacg
    40021 ataaacctgc gccagcgccg ttgcgcccag cgcgttattg cctttgccca aatcaatcag
    40081 cagcagtgcg ttatcttcgg tacaaagctg cggcgtgatg gtgtgacgta catcttccac
    40141 gcgggcaaat gcagaaatca ccagcgacag cggcgacgtc atttcgcgct cttcgttacc
    40201 ttcctgccag cgggttttca tcgacataga gtctttaccc accgggatcg tcaggcccag
    40261 cgccggacaa agttcttcac ccacggcttt aacggcttca tacaggcccg catcttcacc
    40321 agggtggcct gccgccgcca tccagttggc ggaaagttta atgcgtttga tatcgccaat
    40381 ctgtgttgcg gcaatattgg ttaacgcttc accgaccgcc agacgggcag aggcggcgaa
    40441 atccagcagc gcaaccggcg cacgctcgcc aatcgccatc gcttcaccgt agtagctgtc
    40501 gaggctggcg gtagtgaccg cgcagttagc gaccggcacc tgccacggcc ccaccatctg
    40561 atcgcgcgcc accatgccgg ttacgctgcg gtcaccgatg gtcaccagga aagttttctc
    40621 cgccacggtc ggcagatgca gtacgcgttt caccgcgtct gcaatggtga tcccttcacg
    40681 gaccagcgca tcgcccttcg ctttcagcgt ttgtacatcg cgggtcatct tcggcgtttt
    40741 accaagcagg acgtccagcg gcagatcgat cggctgatta tcaaaatgac gatcgtgcag
    40801 agaaagatgc agttcttcgg tcgcttcacc aatcaccgcg tagggtgcgc gctcacgctt
    40861 acacagttcg tcaaacagcg gtaattgatc ggcagcaacc gccagcacgt agcgttcctg
    40921 ggattcgtta caccagattt ccagcgggct catgcccggt tcgtcgctta gaatatcgcg
    40981 cagttcaaat ttaccgccgc gtccgccgtc gctcaccagc tccggcatcg cgttagaaag
    41041 accgcccgca ccgacgtcgt ggataaacag aattgggtta gcatcaccaa gctgccagca
    41101 acggtcgatc acttcctggc aacgacgctc catctccggg ttgtcgcgct gtacggaagc
    41161 aaagtcgagg tcggcatcag actgaccaga cgccatggaa gacgccgcgc cgccgccaag
    41221 accgatgttc attgccgggc cgccgagaac gaccagcttc gcaccgacgt tgatctcgcc
    41281 tttttgtacg tgatcggcgc gaatgttgcc gatcccgccc gccagcatga tcggtttgtg
    41341 ataaccgcgc agctcttcgc cattgtggct gttcactttt tcttcataag tacggaagta
    41401 gccgttcagt gccggacgac caaattcgtt gttaaacgcc gcaccgccca gcgggccttc
    41461 ggtcatgatg tccagcgcgg taacaatgcg ctcaggctta ccgaaatctt cttcccacgg
    41521 ctgttcgaag ccaggaattc gcaggttgga tacggagaaa ccaaccaaac cagctttcgg
    41581 cttcgcgccg cgcccggtgg caccttcatc gcggatttca ccgccggaac cggtcgccgc
    41641 gcccggccac ggagaaatcg ccgtcgggtg gttgtgagtt tcgactttca tcaggatgtg
    41701 cgccggttcc tgatggaaat tgtagcggcc cgtttcgtgg tcagcaaagt agcggcccac
    41761 ttcagaacct tccattacgg cggcgttatc tttataagca gagagaacgt gatctggcgt
    41821 ggtttcgaaa gtatttttga tcatcctgaa cagcgatttc ggctgttgtt caccgtcgat
    41881 aatccagtcg gcgttaaaaa ttttgtggcg gcagtgctca gagttcgcct gagcgaacat
    41941 atacagctcg atgtcgttcg ggttacgacc aagctttgtg aaagcatcct gcagatagtc
    42001 aatttcatct tccgccagag ccaagccaag acgcaggtta gcgtcgatca gcgcctgacg
    42061 gccctgcccc agcaaatcaa cgcttgtgac cggagtcggt tgatggtggg caaacaactg
    42121 ctctgcatca tctaaatcaa aaaagaccgt ttccatcatg cggtcgtgca gttcagcggt
    42181 aacctgctgc cattgttcat tggtcagcgt accggcttct atatagtaag caacgccgcg
    42241 ctcaaggcgg tttacctgtt gtagcccgca gttatgggca atatcggtcg ctttcgaaga
    42301 ccagggagag atggtgccag gacgcggggt caccagcagg agtttgcctt gcggggcgtg
    42361 gctggcgagt gccggaccat atttcagcag gcgttcaagt tgtgcgtgct catcatcgtt
    42421 taacggcgca ttgaggtcag caaaatggac atactcggcg taaatattgt gaaccgggag
    42481 cctggcagcc tgaaaacgtg ccagcagttt gttgattcgg aatgccgaca gtgcaggcga
    42541 accacgcaga atttccatca taagtctctc gtcttctaag ctttcggtgt acccaagggg
    42601 ggggaaacgg gcgtcattat aaagaatctg atgcgctgac gaaaccgttt gcgtggaaat
    42661 aaaatcacca tcgtgaatta gcaacgcgtg ccgccaatgg ctgtaataag ttgccatctg
    42721 gcgcaggttt acgcaaaatg ccgctcattt atgagtaaac ctttcactat tattacgttt
    42781 tttcaagctg ggacgcgcac gacacagaga attaactaat tgaaaaaatt aaagattaat
    42841 tatctgttca tcggcattct ggcactgctg ctcgcggtcg ctctctggcc atccattccc
    42901 tggtttggta aagccgacaa ccgtatcgcc gccattcaag cgcggggaga gttgcgtgtg
    42961 agcaccattc atactcccct gacttataac gaaatcaacg ggaaaccttt tggcctggat
    43021 tacgaactgg cgaaacagtt tgccgattac ctcggcgtaa aactgaaagt gaccgtgcgg
    43081 cagaatatca gccagctgtt tgacgacctt gataatggta acgccgacct gctggcggca
    43141 ggactggtct ataacagtga gcgggtaaaa aattatcagc ctggccctac ctattattcc
    43201 gtgtcacaac aactggttta taaagtgggt cagtatcgcc cacgtacgct gggcaacctg
    43261 acggcggagc aactcaccgt tgcaccgggt catgtggtag ttaacgatct ccagaccctg
    43321 aaagaaacaa aattcccgga attaagctgg aaggtagacg acaaaaaagg ctctgcggaa
    43381 ttaatggaag atgtcatcga aggaaaactc gattacacca ttgctgattc tgtcgccatc
    43441 agcctgtttc agcgcgttca cccggagctc gccgtagcgc tcgatatcac cgatgaacaa
    43501 ccggtgactt ggtttagccc gttagatggc gataataccc tttccgccgc cctgctcgac
    43561 ttcttcaacg aaatgaatga agacggtacg ctggcacgca ttgaagagaa atacctgggg
    43621 catggcgatg attttgatta cgtcgatacg cgcacatttt tacgcgccgt cgatgcggta
    43681 ctgccgcagt taaagcccct gtttgagaaa tacgccgaag aaattgactg gcgtttgctg
    43741 gccgctattg cttatcagga atcgcactgg gatgcacagg ccacttcacc gacgggtgtg
    43801 cgcggcatga tgatgttaac caaaaatacc gcgcaaagcc tcggcattac ggatcgtacc
    43861 gatgccgaac agagcatcag cggtggcgtg cgttatttgc aggatatgat gagtaaagtg
    43921 ccggaaagtg tgccggagaa cgaacggatc tggtttgccc tcgctgcgta caatatgggc
    43981 tatgcgcata tgctggatgc ccgcgccctg acggcaaaaa ccaaagggaa tcctgacagt
    44041 tgggctgacg taaaacagcg tctgccttta cttagccaga aaccctatta cagcaagctg
    44101 acttacggct acgctcgtgg gcatgaagcc tacgcttatg tcgaaaatat tcgtaagtat
    44161 cagattagcc tggtgggtta tctgcaagag aaagagaagc aggctacaga agcggcgatg
    44221 caactggcgc aggattatcc ggcggtatcg cctacggagt tgggcaaaga gaaatttcct
    44281 tttctctcgt ttctttccca gtcgtcatca aactatttga cccattctcc ctctctgctg
    44341 ttttccagga aagggagtga agagaaacaa aattaatccg tcgaggattg cgcttttttc
    44401 tgcgctttaa tttcctggcg gcgcatgcga aagaagtcac tgagcaacgc cgcgcactca
    44461 tccgccagta ttccttccgt aatttccact cggtgattca tacccggatg atgcagcaca
    44521 tccattaaag atcccgcagc gccagttttc gcgtcacgcg caccaaagac cacgcgacca
    44581 atgcgactgt ggatcatcgc tccggcacac attacacatg gttcaagcgt gacatacaac
    44641 gtggcgtcga tcagacgata attttgcatc accagaccac cctgccgcag ggccatgatt
    44701 tctgcatgtg cggtgggatc atggcgacca atcgggcggt tccagccttc gccgattacc
    44761 cgattgttat gcactaatac cgcgccgacc ggcacttccc gctcatccca ggcacgtttc
    44821 gccagcgtca gcgcgtgacg catccaatat tcgtggctaa attcgacttc agacaaaaag
    44881 aaaactccgg ttataaaagc gcggcgcatt atacacggac gctatgcttt actctattcc
    44941 agttgctgga gttcaccgcg cggggttact cgccaacgat gctgacagaa ataaagcagc
    45001 ggattgtcct gattactgtc gctatagcca ctgtacagcc gcagcggcgt gccgattttg
    45061 cgctccagtt gtgcgacctt ttcatgtccc agacaacgca tcgtcaatac ccaaccacca
    45121 tagccacgct gaatttggct ggcgataaga ttaacccgcg gcagccaggg cgtatcgaaa
    45181 taaaccgctt caaccagcgg ctgcggagag ccggtaatca accagatatc agcatcggaa
    45241 cttaacaggt aggtggttaa tcgctcctga accagcggaa aggcggtaac attgtcgcga
    45301 aaccagcgca cgaaatcggc ctgcaacgtc tgtaaacgtg cttcgctgtg accaaaagtg
    45361 cacccccaca gaagcagact catcggccag cgtgccgcac gaccttttat caataacgca
    45421 atggctataa tcggtaacaa cggcaggaca agtaacgcat tcagcggttg gcgacgcagt
    45481 aaatagcgca gaaaactgcc gaacatatcc tgctgatgca atgttccatc taagtcaaaa
    45541 aacaccacac gacgctcgtg agttgccaaa ccatactcct ctggactgaa acatcctgat
    45601 taattttcgc tactacatag cctaacagat agatcatcac ttttccggca acaatcgacc
    45661 gcatcatggc taactggaat ttttaattca tggcaattag cggcaatgga atataaaatt
    45721 cactcgcgtg tgtctcatat tgcctgttgt cgccactatt tcatcgcgag gaaagatatg
    45781 aacggcttac ttcgtatccg tcagcgttac caagggcttg cccagagtga taaaaaactg
    45841 gcggattatc tgctgctaca acctgatacg gcgcgccatt taagctctca gcaactggcc
    45901 aacgaagccg gagtcagtca gtccagcgtc gtgaagttcg cgcaaaaact cggctataaa
    45961 ggttttccgg cgcttaagtt ggcgttgagt gaagcgctgg caagccagcc ggaatcgccc
    46021 tccgtgccca ttcataacca aatccgcggt gatgatccgt tacggctggt cggcgaaaaa
    46081 ctgattaaag aaaataccgc cgctatgtac gcaacgctaa acgttaatag tgaagagaaa
    46141 ctgcatgaat gcgtaacaat gttgcgctct gcgcggcgga taattctgac cggtattggc
    46201 gcttcgggtc tggtggcgca aaactttgcc tggaagctga tgaagattgg cttcaatgct
    46261 gccgcactgc gcgatatgca tgcgctactc gcaacagtac aggcgtcgtc ccctgacgat
    46321 ctgttattag ccatttccta caccggtgta cgacgcgagt taaacctggc ggcagatgag
    46381 atgctgcgag tgggcggaaa agtgctggcg attaccggct ttactccgaa tgccctgcaa
    46441 cagcgtgctt ctcattgcct gtataccatt gccgaagaac aagcgacaaa cagtgcttca
    46501 atctctgctt gtcacgctca gggaatgtta acggatttgc tgttcattgc gctgattcag
    46561 caggatctgg aactggcacc agaacgtatt cgtcatagtg aagcgctggt gaaaaaactg
    46621 gtctgagtaa agaatgcgcg tataatgccc gcccggtttg tgttgttttg agagtttcct
    46681 tatggcgctg ttaatcacta aaaaatgcat caattgtgat atgtgtgaaa tcaaataaaa
    46741 acacatttat tttcatagtg atacagcaat gtgttattga atgtgttgtt ggatttatgg
    46801 tgcaaaaaaa ttttgcataa gttcagcaac acaaagaaca ccactttctt taacgtcctc
    46861 cgcgccctaa aggacggggc tttacggcgc aaaaggcctg tgacatacaa cattaacttt
    46921 tcagtcaacc gtagcacgtt cttgtatacg ccgtgtctgc ggttttatat aaccctggct
    46981 ggaaacctct tatacaaagt cgattcgcca acatcataga tgatcgccac cttctggcga
    47041 gaaactcctg atgcaattag tcgcccggcc tgcgcccatt attctggtga aagtttggga
    47101 cggcgaccgc caattcgtcc ctgtgcgcga gcagcttcca gtcctgcttt tgttcgttca
    47161 acaatcagtt cacgttccat ttcagccagg gcacccatca catggaagaa aaagcgtccc
    47221 attggtgtgc tggtatcaat tgaatcagtc agactacgaa agttgatgcc tcgttcgcgc
    47281 aacgcctcca ccagcacgac aagatgccgc atactgcgcc ccagccgatc cagcttccag
    47341 acaaccagtg tgtcgcctgc cgataatgtc ttgagcagtt ttttcagccc cggtctttcg
    47401 gactttgtac cacttatctt gtcttcaaaa atcagctcgc atcctgcaca gttcaacgca
    47461 ttacgt
//
LOCUS       DAEBXN010000023        46473 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.023, whole genome shotgun sequence.
ACCESSION   DAEBXN010000023 DAEBXN010000000
VERSION     DAEBXN010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46473
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.023"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(26..1885)
                     /gene="gss"
                     /locus_tag="KJL99_001273"
     CDS             complement(26..1885)
                     /gene="gss"
                     /locus_tag="KJL99_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HBD4436059.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            2090..2956
                     /gene="yghU"
                     /locus_tag="KJL99_001274"
     CDS             2090..2956
                     /gene="yghU"
                     /locus_tag="KJL99_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HBD4436060.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPFSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3079..3327)
                     /gene="hybG"
                     /locus_tag="KJL99_001275"
     CDS             complement(3079..3327)
                     /gene="hybG"
                     /locus_tag="KJL99_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HBD4436061.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3461..3802)
                     /gene="hypA"
                     /locus_tag="KJL99_001276"
     CDS             complement(3461..3802)
                     /gene="hypA"
                     /locus_tag="KJL99_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HBD4436062.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3795..4283)
                     /gene="hybE"
                     /locus_tag="KJL99_001277"
     CDS             complement(3795..4283)
                     /gene="hybE"
                     /locus_tag="KJL99_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HBD4436063.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRRGE
                     NA"
     gene            complement(4276..4770)
                     /locus_tag="KJL99_001278"
     CDS             complement(4276..4770)
                     /locus_tag="KJL99_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HBD4436064.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(4770..6473)
                     /gene="hybC"
                     /locus_tag="KJL99_001279"
     CDS             complement(4770..6473)
                     /gene="hybC"
                     /locus_tag="KJL99_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HBD4436065.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6470..7648)
                     /gene="hybB"
                     /locus_tag="KJL99_001280"
     CDS             complement(6470..7648)
                     /gene="hybB"
                     /locus_tag="KJL99_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HBD4436066.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIGLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7638..8624)
                     /gene="hybA"
                     /locus_tag="KJL99_001281"
     CDS             complement(7638..8624)
                     /gene="hybA"
                     /locus_tag="KJL99_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HBD4436067.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8627..9745)
                     /gene="hybO"
                     /locus_tag="KJL99_001282"
     CDS             complement(8627..9745)
                     /gene="hybO"
                     /locus_tag="KJL99_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HBD4436068.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGSAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEKGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9934..10221)
                     /locus_tag="KJL99_001283"
     CDS             complement(9934..10221)
                     /locus_tag="KJL99_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HBD4436069.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10340..11227)
                     /locus_tag="KJL99_001284"
     CDS             complement(10340..11227)
                     /locus_tag="KJL99_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HBD4436070.1"
                     /translation="MPRLTAKDFPQELLDYYDYYVHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVG
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPPYEAALKDNNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            11384..12424
                     /gene="gpr"
                     /locus_tag="KJL99_001285"
     CDS             11384..12424
                     /gene="gpr"
                     /locus_tag="KJL99_001285"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HBD4436071.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12464..12958)
                     /locus_tag="KJL99_001286"
     CDS             complement(12464..12958)
                     /locus_tag="KJL99_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HBD4436072.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLKWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            13149..14033
                     /gene="yghA"
                     /locus_tag="KJL99_001287"
     CDS             13149..14033
                     /gene="yghA"
                     /locus_tag="KJL99_001287"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HBD4436073.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPSIQAKMTPIPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASVITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(14305..14730)
                     /gene="exbD"
                     /locus_tag="KJL99_001288"
     CDS             complement(14305..14730)
                     /gene="exbD"
                     /locus_tag="KJL99_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HBD4436074.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(14737..15471)
                     /gene="exbB"
                     /locus_tag="KJL99_001289"
     CDS             complement(14737..15471)
                     /gene="exbB"
                     /locus_tag="KJL99_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HBD4436075.1"
                     /translation="MGNNLMQTDLSVWGMYQYADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            15723..16910
                     /gene="metC"
                     /locus_tag="KJL99_001290"
     CDS             15723..16910
                     /gene="metC"
                     /locus_tag="KJL99_001290"
                     /EC_number="4.4.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HBD4436076.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            17050..17709
                     /locus_tag="KJL99_001291"
     CDS             17050..17709
                     /locus_tag="KJL99_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBD4436077.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGSWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(17749..18648)
                     /gene="yqhC"
                     /locus_tag="KJL99_001292"
     CDS             complement(17749..18648)
                     /gene="yqhC"
                     /locus_tag="KJL99_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HBD4436078.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            18842..20005
                     /gene="yqhD"
                     /locus_tag="KJL99_001293"
     CDS             18842..20005
                     /gene="yqhD"
                     /locus_tag="KJL99_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HBD4436079.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGTGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            20110..20937
                     /gene="dkgA"
                     /locus_tag="KJL99_001294"
     CDS             20110..20937
                     /gene="dkgA"
                     /locus_tag="KJL99_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HBD4436080.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGKIAKLDQGKRLGPDPDQFGG"
     gene            21137..22063
                     /locus_tag="KJL99_001295"
     CDS             21137..22063
                     /locus_tag="KJL99_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HBD4436081.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     VDHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPESFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            22114..22371
                     /gene="yqhH"
                     /locus_tag="KJL99_001296"
     CDS             22114..22371
                     /gene="yqhH"
                     /locus_tag="KJL99_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HBD4436082.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(22414..24633)
                     /locus_tag="KJL99_001297"
     CDS             complement(22414..24633)
                     /locus_tag="KJL99_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708831.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HBD4436083.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTLGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VIVQPPRQKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEGVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(24744..26156)
                     /gene="ftsP"
                     /locus_tag="KJL99_001298"
     CDS             complement(24744..26156)
                     /gene="ftsP"
                     /locus_tag="KJL99_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HBD4436084.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(26231..26968)
                     /gene="plsC"
                     /locus_tag="KJL99_001299"
                     /gene_synonym="parF"
     CDS             complement(26231..26968)
                     /gene="plsC"
                     /locus_tag="KJL99_001299"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HBD4436085.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(27202..29460)
                     /gene="parC"
                     /locus_tag="KJL99_001300"
     CDS             complement(27202..29460)
                     /gene="parC"
                     /locus_tag="KJL99_001300"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HBD4436086.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(30006..30488)
                     /locus_tag="KJL99_001301"
     CDS             complement(30006..30488)
                     /locus_tag="KJL99_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBD4436087.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDAPATTAPEAFRFDICGSVSELIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(30541..30933)
                     /gene="ygiW"
                     /locus_tag="KJL99_001302"
     CDS             complement(30541..30933)
                     /gene="ygiW"
                     /locus_tag="KJL99_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HBD4436088.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSVTQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            31085..31744
                     /gene="qseB"
                     /locus_tag="KJL99_001303"
     CDS             31085..31744
                     /gene="qseB"
                     /locus_tag="KJL99_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HBD4436089.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLNLTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            31741..33015
                     /gene="qseC"
                     /locus_tag="KJL99_001304"
     CDS             31741..33015
                     /gene="qseC"
                     /locus_tag="KJL99_001304"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HBD4436090.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNGPGVTPEALARIGERFYRPPGQTATGSGL
                     GLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(33061..33393)
                     /locus_tag="KJL99_001305"
     CDS             complement(33061..33393)
                     /locus_tag="KJL99_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HBD4436091.1"
                     /translation="MLKQKIKTIFEALLYIMLTYWLIDSFFAFNKYDWMLESGGNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWLYVFSLVICLVCGWWLF
                     WGRFIFCY"
     gene            33712..34293
                     /gene="mdaB"
                     /locus_tag="KJL99_001306"
     CDS             33712..34293
                     /gene="mdaB"
                     /locus_tag="KJL99_001306"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HBD4436092.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYNVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGSLVQDKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            34324..34638
                     /locus_tag="KJL99_001307"
     CDS             34324..34638
                     /locus_tag="KJL99_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HBD4436093.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(34686..36578)
                     /gene="parE"
                     /locus_tag="KJL99_001308"
     CDS             complement(34686..36578)
                     /gene="parE"
                     /locus_tag="KJL99_001308"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HBD4436094.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQGLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(36607..37188)
                     /gene="yqiA"
                     /locus_tag="KJL99_001309"
     CDS             complement(36607..37188)
                     /gene="yqiA"
                     /locus_tag="KJL99_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HBD4436095.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(37188..38015)
                     /gene="cpdA"
                     /locus_tag="KJL99_001310"
     CDS             complement(37188..38015)
                     /gene="cpdA"
                     /locus_tag="KJL99_001310"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HBD4436096.1"
                     /translation="MESLLTLPLAGEARGRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(38040..38462)
                     /locus_tag="KJL99_001311"
     CDS             complement(38040..38462)
                     /locus_tag="KJL99_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HBD4436097.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(38463..39092)
                     /gene="nudF"
                     /locus_tag="KJL99_001312"
     CDS             complement(38463..39092)
                     /gene="nudF"
                     /locus_tag="KJL99_001312"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HBD4436098.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            39297..40778
                     /gene="tolC"
                     /locus_tag="KJL99_001313"
     CDS             39297..40778
                     /gene="tolC"
                     /locus_tag="KJL99_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HBD4436099.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLEREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            40926..41597
                     /locus_tag="KJL99_001314"
     CDS             40926..41597
                     /locus_tag="KJL99_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HBD4436100.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            41603..42763
                     /locus_tag="KJL99_001315"
     CDS             41603..42763
                     /locus_tag="KJL99_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HBD4436101.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDEIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(42801..43589)
                     /gene="ygiD"
                     /locus_tag="KJL99_001316"
     CDS             complement(42801..43589)
                     /gene="ygiD"
                     /locus_tag="KJL99_001316"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HBD4436102.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     LNPTPEHYLPLLYVLGVWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            43732..44505
                     /gene="zupT"
                     /locus_tag="KJL99_001317"
     CDS             43732..44505
                     /gene="zupT"
                     /locus_tag="KJL99_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HBD4436103.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQLLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(44563..44733)
                     /locus_tag="KJL99_001318"
     CDS             complement(44563..44733)
                     /locus_tag="KJL99_001318"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4436104.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(44994..45647)
                     /gene="ribB"
                     /locus_tag="KJL99_001319"
     CDS             complement(44994..45647)
                     /gene="ribB"
                     /locus_tag="KJL99_001319"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HBD4436105.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(45751..45899)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            46021..46311
                     /gene="ubiK"
                     /locus_tag="KJL99_001320"
     CDS             46021..46311
                     /gene="ubiK"
                     /locus_tag="KJL99_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HBD4436106.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELENRSTEIKKQPDPETLPPTL"
BASE COUNT        11557 a        12413 c        11577 g        10926 t
ORIGIN      
        1 cagtacgggg ttgctacaac atgtattact ttttcaccac aattaacggt tcaatatcac
       61 tctctttttt gatgaccagt gattcatcac cgcgcaaaca cgtcccaccg tagttgccgc
      121 caacggtgaa ggtacatacc tgaatgtatt taccgtccac tttcggcaaa caccacagtt
      181 gctgatagat gtttttctgc tcggcaaatt taccgctggt tttgtccagc acctcttcat
      241 gatggctgac caggtcgata ttgctaccac agcgaccggc gatcggtttc actgcgtaac
      301 ctgttttcac caattcatca ttaacagtga aatcggtatc cagcaggtaa cgatggtgcg
      361 ggaacagcga ccagaggatc ggcagaatcg ctttgttgcc ggggatcacc gtccacagcg
      421 gctcaaagac cagcacttcc gggcgcagca atacgtcgat aagacgcact tcgttttgcg
      481 gatgaccggt acggattggc accgcagcaa actcacggtc gctaacttca cgaatctgat
      541 caaacgcggt ttcccacgcc caggttttcc acacgcagtt aaccagtcgc ccttccccat
      601 caatcagttg cccggcagca tcccagccca gttcatccag cccgcgcaag atacgcgttt
      661 caaagcccgc ctggtgcagc gcctgctcca taaactgcgc gtgatagttt tcctcgatat
      721 ctttgtcctg catgatatgg acaaacggac gtgcacgact gtgtttccag gcaccagcca
      781 gttcgttaat cagcccttcc gccggattga agccgttgcc tttatagccc tgctccgccc
      841 aacgttcgag gatcaagccc gcttcggtat gacaggaggc ggagtcggcg ttgtattcgt
      901 aaaccttcag gccacgctca tccatgcaga aatccatacg accagtgatc atatggtgac
      961 ggcgacgctg ccaggagaga cgcaaacgtg gccagaggat tttcggaatg tcgaacagcg
     1021 ccagcaggtt gtcatctttc agcaccttgt cggttgcgtg aagatacatc agatgcagct
     1081 cgttggtggc tttaattagc tcctgctcgg cactctcggt aatggtgtag taatgatacg
     1141 gatcctgatt gatcacctga ccgttggcct gcacataggc gttttgtagc ggatcttttt
     1201 catccagcca tttaccgtca aactggcctt tgttttccag acgcgctcca ctgattttca
     1261 gcagctcgcc tgcaatttcc ggctgcggta agctgtattc agtatcttcc gtctggatca
     1321 tccagcccag aatggtggtg tcatcaaaag tgtctttaag ggtatagcag ccgttttcga
     1381 ccaccatctc cagctcgcgc gtccactgtt gcccttgcgg caacggggaa tgaatcacgt
     1441 tctgttccgc aatacggact ttgttgccat gcaattgggt aatgatggcg acatggccag
     1501 tgtctttaaa ttcaccgcct ttatcccaga taagaagcgc acccgcgacc ggcgcacgcg
     1561 gcgagccgtt aggaaatgcc tgcaatggca ggatgttgtc attaaccact tctcgcagga
     1621 agcgcagcga gaaaatctcc cacgccatac ccacgtcagt aaagaccaca ccgtaattca
     1681 gaaagagaaa acggcgagca aattcaacgc attgccactt gtggcccata tattcgtcgt
     1741 cgatatagct acggaatacg gcgtcatctt cgtattcctg cggatcgaga gaactgtaat
     1801 ctgaagagta gattgctacc ccacctgggg cgtagcccaa taatgtcccg aacggggcat
     1861 cctggctggt cgttcctttg ctcatcactt tacctttaac aatacaacct aagcagttgg
     1921 cgtaattgtt gtgctctgat tacctgctca gcgacattaa ccggacagag gtcagactaa
     1981 tcatacacct catcgcagcg gctggcgcaa agggttaaaa aattcacatt ctgtacagca
     2041 agtgacctgc tacactgctt caacactacc actcagaagg caactcacta tgacagacaa
     2101 tacttatcag cccgcgaaag tctggacgtg ggataaatcc gctggcggcg cgttcgccaa
     2161 tatcaatcgc ccgttttctg gtccgacgca tgaaaaaacg ctgcccgttg gcaaacatcc
     2221 attgcaactt tattcgctgg gaacgccgaa cggtcagaaa gtaacgatta tgcttgagga
     2281 gctactggcg ctgggcgtta ctggtgcaga gtacgacgcc tggctgattc gtattggcga
     2341 tggcgatcaa ttctccagcg gctttgtcga agtgaaccca aactcgaaga tcccggcgct
     2401 gcgcgatcat acgcataatc cgccgatccg cgtgtttgaa tctggttcga tcctgcttta
     2461 tctggcggag aaatttggct acttcctgcc gcaggatctg gcaaaacgga ctgaaacgtt
     2521 gaactggctg ttctggttac agggtgccgc gccgttcctc ggcggtggtt ttggtcactt
     2581 ttaccattac gcgccggtga aaattgagta cgccatcaac cgctttacca tggaagccaa
     2641 gcgcctgctc gacgtgctgg ataagcaact ggcgcagcat aagtttgttg cgggcgatga
     2701 gtacaccatt gcggatatgg cgatttggcc gtggtttggc aacgtggtgc tcggcggcgt
     2761 gtatgatgcc gcagagtttc tggatgcggg aagttataaa catgtgcaac gctgggcgaa
     2821 agaagtgggc gaacgtccgg cagtgaaacg tgggcgtatt gttaaccgca ccaacgggcc
     2881 gctgaacgag cagttgcatg agcgccatga cgccagtgat tttgagacaa atacagaaga
     2941 taagcgtcag gggtaagggt tggtgttcgt cgcagcaagc catccaggcc ggataaggcg
     3001 ttcacgccgc atccggcaat cgtgcacaat gcctgatgcg acgctgccgc gtcttatcag
     3061 gcctacaaaa gctaatcatc acgcgctggt aatgtcgtaa tccatttggc gcagtgcgtc
     3121 taatgtggct ttggcttcat cttcgtcgat gatgctcatg gcaaatccga cgtgtaccag
     3181 cacccactgg cccagcagat cggcagggtt accttcgcaa atcagggcga tattcacgtc
     3241 gcgcttgata ccacatactt caacctgcgc aagctggtga atatcttcac cgacagccag
     3301 cacctggcct ggaacgccaa tacacattgt taactccggt tatttcgatt gccggacaaa
     3361 cgcgaaaaag aacctttagc gggtgttccc tctcacccta accctctccc cggaggggcg
     3421 aggggaccgt ccgtgcgctt tttaggcgtt gtcctcagtc ttattcaact tcaatacttt
     3481 tgacgatcag cgaatcgccg gtatcgacac gcaaccgctc gccgtgacag agcggacact
     3541 gcgcatcgtg ctgatgaatc tccaccacct ggctgcaatc ccagcaccag gcctgggcgg
     3601 gtttatagac gatatgtaaa tcgcaccctt gcgccaccgt tccgtggcag acaatttcaa
     3661 aactaaaacg gacggcgctc tcctcaacgc aggagagcgc gccaatttcc agccacacgg
     3721 cggtgacgcg cttaacatcg tgctgctccg cctgccgttg gataatttca acggcgctct
     3781 ggcaaagcga caactcatgc attttcgccc ctcctgcggc caaacagcag ggcgcgacgc
     3841 cctgcgtgtg gtacatccgg attggtgact ggcaacgaaa ggatcattcg tgcgcagtca
     3901 tccgtcaggc gttggccttc ttcaatcgac atgctgtgcg aaagcggcga catcagcgaa
     3961 caggagaggt attgcgaaac accgtccagt tcgccaacgg taaaggtcat ggtgccatac
     4021 ggcagttgca gaccaatttt ttcactcact ttgcgcagcg gccagagttg atccgggccg
     4081 gggaagataa ctgcactcag catccacggg gtgatcacac accccgtcca ctgaccttcg
     4141 aacagcgtaa aatcagaaac atacaccggc attgaaggat gcagaaaaga gagatcgtgc
     4201 atcgaacgcc gggcaatttc ttcaaacgct gcctgtactt gcgccttcgg ggaggtctgg
     4261 aaacctgcta tctcttcagt catgaatcgc ctcccgtggg atggcttcca cgcctgattc
     4321 gcgcagcgca gccagaacct gctcaagcgc aggttcaatc attgcttcaa ccgtcggcgt
     4381 taagccgatg tgtggctcca gcgattccgg gatcacgccg accagggtca gctttttcgg
     4441 aaactcaccg gtgaagcgca gggccgacaa tacgtcggcc aggccaagct gatgcggaga
     4501 gattttgttg gtaaacaacg ccggaacttc ttcatcccgc aggatcatca tcgttcccgg
     4561 agtgttcttt ttcgacacaa tggcatccgc aataatcaga tgatcgcgat ttgccatgtc
     4621 gccaagcagc tccattcccg ccgtgccgcc atcgaggatc tcaacataat ccggcagaat
     4681 gtatcgttgc tctaacgctt cgacaatccg cacgccgatg gcttcatcgg tcagcaaaat
     4741 attgccgacc cctaagacta aaatacgcat tacagaacct tcactgaaac cacttcgttg
     4801 ccgtcggcat ccactacgtg taccgcacag gccatgcacg ggtcgaagga gtgaatggta
     4861 cgcaccactt ccagcggttt attcggatcg gcaaccggtg tacccaccag cgactgctcg
     4921 taaggaccga cgtcatcatt gaagttacgc ggaccagagt tccaggttga tggaacaacc
     4981 gcctggtagt tgctgatgat accgtcttta ataaccatcc agtgagagag cataccgcgc
     5041 ggcgcttcga ggaagccaac acctttgaac tcacccgttg ccggaatgtt cggcttcaca
     5101 aaggtggtgt gatcgccttt gccgatattg gtgatcagtg cactgtattg gttttgcagg
     5161 atatcctgca attcacagca gtgaacggta cgaccaataa tacggcccag cgtggagtgc
     5221 agctgtgcca cttccagcgt gttgccagtc agtttctgat aaatcgcaac gatttcattc
     5281 agtttgttct gggtagattc gcgacctgcc gccagtttca ccagcatatt agccagcgga
     5341 cccacttcta ccgttttgcc gtagaaagtc ggtgatttca cccaggaata tttcccgtcg
     5401 tcagaccaac catcataagc cggaatggtg gtaccttccc acggcgcctg tggcgcttcg
     5461 tctttatacc aggagtgctt cgcgctttcc tgaatgcctt tgatcaggta ttcatcggaa
     5521 tgagaagtga tcggacgata cgaggacaga tccgcattct caatgtagcc tcccgggaac
     5581 aggaagctgc cgtttttgct gtcggtcggg aattccggca cgctcaggta gttcaccgca
     5641 cctttaccgc gcgtcagcca ttccgggtag aacgcggcga taaccgcggt atcaacctta
     5701 taaacctgct caacaaagtc gctcagtttg tcgatgaaag acttgatgta catcaggcgc
     5761 tcaaggttca acacgcccaa cccgtcgagg ttgattgggt tcgcgacacc acccacagcc
     5821 aggttctgaa tgtgcggcgt tttaccgccc agcagcgcca cgacgcggtt agcgtcacgc
     5881 tggcactcca gcgcctgcag gtagtgcgct accgcaatca ggttcacttc cggcggcagt
     5941 ttcatcgccg ggtgacccca gtagccattg gcgaaaatac ccaactgacc gctggcaacc
     6001 agatctttga tcttgttctg aactttagtg aactcttccg gactgttcag atgccaggtc
     6061 gaaacgcctt tcagcatttc ggaggctttg gttgggtcag cttgcagtgc agaagtgatg
     6121 tccacccagt ccagcgccga aagctgatag aaatgaacga tatggtcatg cgtggtgtgc
     6181 gcagccagaa tgatgttacg gatgtattgc gcgttaaccg gaacgtcgat attcagcgca
     6241 ctttctgccg cacgaacgga agacagcgcg tgagtggtag tacatacgcc acagatacgt
     6301 tgcacaatca tccatgcatc gcgcggatcg cggtttttca cgatctcttc catgccgcgc
     6361 cacatggtac cggaagccca tgctttcgaa acgacgccat tttcgatttc gcaatcgatg
     6421 cgtaaatgcc cctcaatacg ggttaccgga tcaatagtaa ttctctggct catgctttgc
     6481 tcgcctcatg acgattatga tcgttttgtt ttaaaggagg aagtatcggc agtagacgaa
     6541 tgagtacgat gtaagcgcaa atctcaatag ccacaaaacc aatagaaatc aacagttctt
     6601 cccaggtcgg gaagtaggcg taaccgccgc ccgggttgaa tgccaccaac gaataggtca
     6661 gacgccaggt tgcacaacct aacagtgcgc tcagtgctga caggaacagc atacgggaat
     6721 cattacgcag cttcgccaca cgcagaacga ccagcgggaa gagcatcagc aggacttcaa
     6781 tccagaacat cacggagtag aagtcaccgg caaacgctaa cgacagcttg tcgcgataga
     6841 taagctcgcc aaagcgcagc ccgatgaaaa tcgccagcaa cacactgatg gtgttggtca
     6901 gcttgacgaa caggctcttt tcatccggac cgttgccacg cagacccgcc tgcaccagtg
     6961 aaccttcaaa gatgacaatc gagaagccca tgatgaacgc cgtcagcagc gagaacagcg
     7021 gcaacatttc atagctctgc cagagcggat gcaccttgta gcccgccgag atcatcagcg
     7081 accccattga agactggtgc atggtcggca gcagcgcacc gagcgcgatg atgaagaaca
     7141 tcaccttgtt cagacgttgt agcgacacct tccagcccag acgttcaaac agtgccggag
     7201 caaactccag agccatcacg ccgatgtaga tggtcataca gaccgccgtc tcgaacagta
     7261 ccgagttcac gttgaagtga cccggaatgt agaagtacgg caggttccag tagcgaccca
     7321 cgtcgatagt gatcgacaag ccacccagtg agtaaccaaa cagactcgcc aacagcgccg
     7381 gacgcaccag cggatggtat tgcccacggt taaagacgta taccgcccac gccagcgccc
     7441 agccgccaca ggcaaagccg gtgccaatca gcaggtcaaa cgcgatccac acgccccacg
     7501 ggaagccgcc gttcaggtca gagacagagc ccagaccgaa caccagacgc ttcacaatca
     7561 ggagcataca gatgacgatt aacggtccaa aaatcatgac cggtttactg atgattttgc
     7621 cgcccagcgg ttgtggatca tgactcatga tcgtctcctc cgtcgtgatg gtcgtttttg
     7681 gtgttgcgac gaaccagcac ggttaagccc gccagcacag ccagtggtag catcatgcct
     7741 ttatacaggg tgtgttgaat attttcggaa cgcgcaccgg tagaaagatc gtccagtttc
     7801 ggcaggtcga gattttcata aggcacaccc gtcagtacca gaacctgagt accgccgccc
     7861 tctttctcgc cgtacagatg cggataatat ttcggcaccg tatgcaggta agtgtcgcca
     7921 gatttcagcg tctgacgcgg atagtggtat tcgctgccag gcttcagcgc cagacgtttt
     7981 ttcgcctccg ccatcagctc ttcacgcgta ccgaaaatca ccgcgcccgc cgggcacact
     8041 tctacgcagc caggcagacc gcctttatcg agacgttcca cacctttctg gttgcacagc
     8101 tcgcacttat gcagcgcacc aaacgggttg ttgtagtcgt acttcggcac gttgtacgga
     8161 caggcgacca tgcagtaacg gcaaccggtg cacacgtctt tgtcgtaatg gacaatgccg
     8221 gttttcggat cttttttcag tgcagagacc gggcacacag agacacagtt cggatcgacg
     8281 cagtgcatac actgtttctt aatgtacgcg tagccgttct cctcctggtc tttgttgacc
     8341 cctgtgccgc tggtccacac ctgaatgatg ttattggtat acggcgacag tttgtcgttg
     8401 ttcgaccagg tctgttcccc ttgcgggtta cgttcaggga agttgatatc ctgacacttg
     8461 gtgacgcaag cctggcagcc tacgcacaag gtcgagtcgt acaacatccc cagcgatccc
     8521 ggaattggcg ggcggttttc agcagccgca tgactgacag acggcagcgc gcccgtcagc
     8581 aatgccccgc aggaggctgc tttaataaaa ttacgtctgt tcacggttat tctccccgtg
     8641 agtcagcgtt atctttcttt tgctgacgac ccagttcacg caccgccatc acgctgacac
     8701 cggcaaccag cccaaccaca ccgccgagca aaccaatagc gcctgcagag acgttgccgc
     8761 cctctttagc gttaacatcc ggtttctgtg aacgcggagt ctgattttcg acgttggcaa
     8821 gctgatggat gcctttatgg aagccgatac ctttttcgtt acagccatag cacgggtgac
     8881 caatcgccac cggccacaca ccgccaacat cgcagaattg cagcgttgag cagttgccgt
     8941 aagtttctgg ccctttacag ccgaggtggt acaagcacca gccttcgcgg tggccttcat
     9001 caccgaactc tttggcaaaa cgaccagcat cgaagtgcgg gcgacgttcg cagtgttcgt
     9061 gaatcagacg gccataggcg aaggtcggac ggtttttgtc atccagtttc ggcggtttgc
     9121 cgtaagtgat gatgtgcgca acggtcgcga ggaagttgtg cgggttcggc gggcagcccg
     9181 gaatattgat aacggttttg cctggcagaa cttcttgcaa gctgactgcg ccagttgggt
     9241 taactccagc tgcggcaaca ccgccccacg cggagcagga accaatagcg ataatggctg
     9301 ctgagccttc cgccgctttg cggatgtgat ccacaatcgg ctcaccggca accatgcaat
     9361 aaataccgtt atcttttaat gggatggaac catccaccac taacacatac tgccctttgt
     9421 acttctcgag agcgttatgt ttgttctctt cgacctgatg accgaaggcg gcggaaagca
     9481 cttcgtgata ttccagagag atagtctcca gcacgaggtt ttctaccgtt ggatgcgttg
     9541 cacgaagcag agattccgta caaccggtgc actcctgcgc gccaatccag ataactggcg
     9601 gacgctgcgg gttagtaacc gattcggcca tctctgcagc ggctttgcta cttaacccca
     9661 tggtggcggc taatgctgca caaagcttca tgaaatcacg acggttaatg ccgtgagaat
     9721 ggatgagggt gttatctcca gtcatttata gttattccgt tgcgagaacc tggcatatat
     9781 tttgcctcaa tcgcaaaatc aataatgcga tcgatgcgcc atttaccaca catttattat
     9841 ggttatcgat atcatgatac tgcggcgggg cgctaaaacg aagggaagaa gcattagtgt
     9901 aattaattaa agcaagataa tacgtatgtt tgatcaattt tcgaggcgat aaccggccat
     9961 aaaaaagcgc aacgtagaac aggaattatt ctcacgaaag aaatccatta ccatctcttt
    10021 atccagtcca tagcggcgcg tcagaatacc cgcttcccag gcgctaagct ggcgatctcc
    10081 ggtcttttcg tacatcctgt gtgagtagcc taatacaaaa ccgcgtttat aatcggcaca
    10141 gaattttgtc acattaaccg cactgtcggc atgcgttgct tttaatcccg ccattaagcc
    10201 tttaccaaaa tggttattca tcgttcacct cattcattat atatcgccat atataacgat
    10261 attttaggca aagaacgact tttgcgcacg ctattttttc atacttgttt aagacgtaat
    10321 cccgtaagag ataaacctat caggagagat atttatcgaa ccatttcagt gtcctttgcc
    10381 aggcaagatc ggcggcagat ttgtcataac ggggcgtgga atcattatgg aatccgtgat
    10441 taacccccgg atagatataa gcctcataaa ccttattatt gtctttcaac gccgcctcgt
    10501 aaggaggcca gccctcgttg attcgggtgt ccagttccgc gaagtggagt agtaaaggcg
    10561 cttcaatctt cgccacatcg gcagtgggtg cctgacgacc ataaaacggc accgcgcagg
    10621 ccagttccgg atacgcgaca gccgccgcgt tcgatacgcc accgccatag caaaatccgg
    10681 taatacccac tttacctgtc gcttgcggat agcgttgcat aaactcaatt gcggcaaaga
    10741 aatcattcat cagtttggtt ggatcaacct gctgttgcag ctcacgacct ttatcatcat
    10801 ttcccggata acctccaacg gaacttaatc cgtcaggtgc cagggcgata taccccgcct
    10861 tcgccactcg ccgtgccaca tcttcgatat acggattcag tccacgattc tcatgcacca
    10921 cccccacggc tggcgttttg ccgctcattt ttgcgggttt caccagataa ccccgtacct
    10981 cgccgtgacc atttggcgaa ggatacgtga tgtactcagc aacaatctcc gggtcggtaa
    11041 actctacctg agtcgccagc gcataatttg gcttgagcaa atcaaacaac gctaatgccg
    11101 tcatcccgcc caccgcatac ttcgccgcaa ggttgaggaa ctcacgtttc gaaattttcc
    11161 cgtgaacgta atagtcgtag taatccaaca actcttgtgg gaaatctttg gcggttaaac
    11221 gcggcatcgt tgcattcctc aactggtgtt tttttaagca aagcataagc gcgtgttttt
    11281 gcccagtttt tcgtcactct gtgagccaga ctacgggata cgcgctggcg aatcgctaaa
    11341 ctagaaacat tgtttcgaaa ttgaacggtg gaaaggagag gtcatggtct ggttagcgaa
    11401 tcccgaacgt tacgggcaga tgcagtaccg ctattgcgga aaaagtggtt tacgcctgcc
    11461 cgcattatcg ctcggtttat ggcacaattt cggtcacgtt aacgcgctgg aatcacagcg
    11521 tgcaatcctg cgtaaagcgt ttgatttagg cattacgcac tttgatttag ccaacaatta
    11581 cgggccgcct ccaggaagcg cagaagagaa ctttggtcgc ctgctgcgcg aggattttgc
    11641 tgcttatcgc gatgaactga ttatctctac caaggctggc tacgatatgt ggcccggccc
    11701 gtacggctct ggcggttcac gtaaatacct gctcgccagc ctcgaccaaa gcctgaagcg
    11761 tatggggctt gagtatgtcg atatctttta ctctcatcgc gtcgatgaaa atacgccgat
    11821 ggaagaaacc gcctctgcgc tggctcatgc ggtacaaagc ggtaaggcgc tgtatgtcgg
    11881 gatctcctct tactcgccag agcggacgca aaaaatggtc gagttgctgc acgagtggaa
    11941 aattccgctt ttaattcatc aaccttcgta caatttactg aaccgctggg tggataaaag
    12001 cggcctgctg gataccctgc aaaataacgg cgtgggctgt attgccttta ctcctctggc
    12061 tcagggattg ctgaccggaa aatatctcaa cggcattccg caagattcac ggatgcatcg
    12121 tgaagggaat aaagttcgtg gtctgacgcc gaaaatgctt accgaagcca acctcaacag
    12181 cctgcgctta ttgaatgaaa tggcacagca gcgtggacaa tcaatggcgc aaatggcgtt
    12241 aagctggttg ctgaaagatg atcgcgtgac gtcggtattg attggtgcca gccgcgcgga
    12301 gcaactagag gagaacgtgc aggcgctgaa taatctgaca tttagcaccg aggagctggc
    12361 gcagattgat cagcatatcg ccgatggcga gctgaatctg tggcaggcgt cgtccgataa
    12421 atgacctgtt aataacgggc gcgcgaaccg cgcccgaatc agatcagtga ttatgacgag
    12481 tcagtcggtc aagatagccc atcacaaatg cagaaagcac aaacgtcaga tggataatga
    12541 cgtaccacat cagtttgtta tccgggacat ttttcgcatc cataaagacg cgcagtaagt
    12601 gaatggaaga aattgccaca atcgacgctg ctactttgtt tttcagcgac gttgcgtcca
    12661 ttttccccag ccacttcagc ttctctttgt tctcggagat atccagctgt gagacgaaat
    12721 tctcataacc ggaaaacatc accatcacca gcaaaccgcc aaccagcgtc atatccacca
    12781 gcgacagcaa cacgaggatc aaatctgatt ccgccatcga gaagatattc ggcagtacgt
    12841 gaataatctc ctggaagaac ttcagcgcca gggcaactaa cgccagcgaa aggccaaagt
    12901 acacgggggc aagcagccag cgagaagcat acattgcatt ttcaagaaaa cgttccatac
    12961 attcctgtcg tgtagttaaa acgggcaaca gtatatcgca attgagtaaa cagcacgcaa
    13021 cagcaaatca acatatacgt taaacctttg tcgcataatt ctgaggttta gggatcttcc
    13081 tggttccctt atcaacgcat cgagagcgtc tacccttaaa ggacgttgaa cctgaaggga
    13141 gaaaaacgat gtctcattta aaagacccga ccacgcagta ttacactggt gaatatccca
    13201 aacagaaaca accgacgcca agcatccagg cgaagatgac accgataccg gattgcggcg
    13261 agaaaaccta tgttggtagc ggtcgcctga aagatcgtaa agcactggtg acagggggcg
    13321 attccggaat aggtcgcgct gccgccatcg cttacgcgcg tgaaggggct gacgtggcga
    13381 tcagttatct tcccgtggaa gaagaagacg ctcaggatgt gaaaaagatc attgaagaat
    13441 gcggacgcaa agccgttctg ctgccaggcg atttaagcga tgagaaattc gcccgttcgc
    13501 tggttcacga agcgcacaag gcgttaggcg ggctggatat tatggcgctg gtcgccggga
    13561 aacaggttgc cattccggat attgcagacc tcaccagcga acagtttcaa aagacctttg
    13621 ccattaacgt tttcgcgctg ttctggctaa cccaggaagc gatccccctg ctaccgaaag
    13681 gtgcaagtgt catcaccact tcgtcaatcc aggcatacca gccaagtccg catttactgg
    13741 actatgcggc tacgaaggcg gcgattctga actacagccg tggcctggca aaacaggtcg
    13801 cggagaaagg tattcgggtg aatattgtcg cgccaggccc tatctggacg gcactgcaaa
    13861 tttccggcgg acaaacgcag gataagatcc cgcagtttgg tcagaaaacg ccgatgaagc
    13921 gtgcggggca accggcggaa ctggcccctg tatatgttta tctggcaagt caggagtcga
    13981 gctacgtcac cgcagaagtg cacggcgtgt gcggcggcga gcatttaggt taaaaaaatg
    14041 cccggttgtg aaaagcaacc gggcatcatt gtgaattcac tcttacctga tgtggcgaaa
    14101 acgccttatc cgcctacaaa cgcatgaaga ttcaacatgt tgcaatagat tttgtaggcc
    14161 tgataagcgt agcgcatcag gcattgagca cctaatgccg gatgcgtcgt gaacgcctta
    14221 tccggcctac aaacgcacga agattcaaca tgttgcaata gattttgtag gcctgatgag
    14281 cgtagcgcat caggcaattc tactttactt cgctttggcg gtttcttcgc cgaccagacc
    14341 tatcttcagg taacccgcct gatgcagcgt atccattacc ttcatcaacg tctcgtaatc
    14401 gacggttttg tccgctcgga agaagatggt ggtgtctttc ttgccttcgg ttaacgcatt
    14461 caacgccgta atcattgttt catcggtgac cggatcgtta ccgataaaca tcgagttgtc
    14521 tgccttcacc gacagataaa ccggtttttc cgggcgcggc tgcggcgtgc tggtagaagc
    14581 aggcaagttc accttcacat ctaccgtcgc taacggtgcc gccaccataa agataatcag
    14641 cagaaccaac atcacgtcga taaacggcgt cacgttgata tcatgcattt cgccgttatc
    14701 gtcgaggttt tcgttaagat gcattgccat cggacattat cctgcgcgta atttttgtgc
    14761 gacacgaacc ggatgcgcag cggcgctggc ttccagatcc aggtcacggc tttgcagcaa
    14821 caatacctgc gctgcaacat cacccagcat cgctttaaag ccgccaatct ggcgtgcaaa
    14881 tacgttatag ataacgaccg caggaatcgc tgccacgagg ccgattgccg ttgctaacag
    14941 agcttctgcg atacccggcg caacgactgc caggttagtg gtctgcgttt gcgcgatacc
    15001 aataaagctg ttcatgatgc cccagaccgt accaaacaga ccaacgaacg gagaaatcgc
    15061 gccgatggtt gccagatagc cgttaccgcg gcccatttga cgacccactg cggcgacccg
    15121 acgctccaga cggaagctgg tacgttcttt aataccttcg ttatcgtcgc tgccttctga
    15181 cagttccagc tcattctgcg cttcattgag caaatgcagg cttaagcttt tgctaccaaa
    15241 atcagcggcg atatcgttgg cctggtttaa ggaacgtgct tcagccagca gttgctgctc
    15301 gcgcttaagg cgacgcttct gattgaagaa ctctacgctc ttactgaaga agattgccca
    15361 ggtgactaca gaggccaaaa taagcccaat catcacgcac ttaacgacaa tatcggcgta
    15421 ctgatacata ccccagacgg aaaggtccgt ctgcattaaa ttattaccca ctgtgtatct
    15481 ccaggacgca agtcacaaaa tctgcgcata ataatatcaa aacgacgtcg aattgatagt
    15541 cgttctcatt actatttgca tactgccgta cctttgcttt cttttccttg cgtttacgca
    15601 gtaaaaaagt caccagcacg ccatttgcga aaattttctg ctttatgcca attcttcagg
    15661 atgcgcccgc gaatattcat gctagtttag acatccagac gtataaaaac aggaatcccg
    15721 acatggcgga caaaaagctt gatactcaac tggtgaatgc aggacgcagc aaaaaataca
    15781 ctcttggcgc ggtaaatagc gtgattcagc gcgcttcttc gctggtattt gagagtatgg
    15841 aagccaaaaa gcacgcgacg cgcaatcgcg ccaatggcga gttgttctat ggacggcgcg
    15901 gaacgttaac ccatttttcc ttacaacaag cgatgtgtga actggaaggt ggcgcaggct
    15961 gcgcgctatt tccctgcggg gcggcagcgg ttgctaattc gattcttgct tttgtcgaac
    16021 agggcgatca tgtgttgatg accaacaccg cctatgaacc gagtcaggat ttctgtagca
    16081 aaatcctcag caaactgggc gtaacgacat cgtggtttga tccgctgatt ggtgccgata
    16141 tcgttaagca tctgcagcca aacactaaaa tcgtgtttct ggaatcgccg ggttccatca
    16201 ctatggaagt ccacgacgtt ccggcgattg ttgccgccgt gcgcagtgtg gtgccggatg
    16261 ccatcattat gatcgacaac acctgggcag ccggtgtgct gtttaaggcg ctggattttg
    16321 gcatcgatgt ttctattcag gccgccacca aatatctggt tgggcattca gatgcgatga
    16381 ttggcactgc cgtgtgcaat gcccgttgct gggagcagct acgggaaaat gcctatctga
    16441 tgggccagat ggtcgatgcc gataccgcct atataaccag ccgtggcctg cgcacattag
    16501 gtgtgcgttt gcgtcaacat catgaaagca gtctgaaagt ggccgaatgg ctggcagaac
    16561 atccgcaagt tgcgcgagtt aaccaccctg ccctgcctgg cagtaaagga cacgaattct
    16621 ggaaacgaga ctttacaggc agcagcgggc tattttcctt tgtgcttaag aaaaaactca
    16681 atgatgaaga gctggcgaac tatctggata acttcagttt attcagcatg gcctactcgt
    16741 ggggcgggta tgaatcgttg atcctggcaa atcaaccaga acatatcgcc gccattcgcc
    16801 cacaaggcga gatcgatttt agcgggacct tgattcgcct gcatattggt ctggaagatg
    16861 tcgacgatct gattgccgat ctggacgccg gttttgcacg gattgtataa cattgccact
    16921 tttggacaat tttgcagaca ttttattgtg aaaagtctta aattgttgcg tccgggatca
    16981 aggcgtcccg gacgattcag gagtacaata ggcagataaa ggcttaaacg ctgttccaca
    17041 ggaaagtcca tggctgttat tcaagatatc atcgctgcgc tctggcaaca cgactttgcc
    17101 gcgctggcgg atcctcatat tgttagcgtt gtttactttg tcatgtttgc cacgctgttt
    17161 ttagaaaacg gcctgctgcc cgcctcattt ttgccaggcg acagcttgtt gatactggca
    17221 ggcgcattga ttgcccaagg ggttatggat tttctgccta cgattgcgat tctgaccgcc
    17281 gcagcaagtc tgggctgctg gctaagttat attcagggga gctggttagg gaataccaaa
    17341 acggtgaaag gctggctggc acagcttcct gctaaatatc accagcgcgc cacctgcatg
    17401 tttgaccgcc acggtctgct ggcgctgctg gctggacgtt ttcttgcatt tgtccgtacg
    17461 ctgctgccaa ccatggcggg aatttccggt ctgccaaacc gccgcttcca gtttttcaac
    17521 tggttaagtg gattgctgtg ggtcagcgtg gtaaccagtt ttggctatgc cttaagtatg
    17581 attccgttcg ttaaacgcca tgaagatcag gtaatgacgt tcctgatgat cctgccaatt
    17641 gccttgttaa ccgctggctt gttaggcacg ctgtttgtgg tgattaaaaa aaaatactgt
    17701 aacgcctgac gattttcccc gttcccggct gctgtaccgg gaacgtattt aattcccctg
    17761 catcgcccgc attcttgccg catcttcccc cggcgtcaca ccgaagtaac gtttaaactc
    17821 acggctaaat tgcgatgcgc tttcatagcc gacgcgcatc gctgctgcgc tggccttcat
    17881 gccgtcatgg atgatcatca tccgcgcctt atgcagacgg taattcttca aatactgcaa
    17941 cggcgaggtg ctggtgacag acttaaaatt atggtggaac gccgatacgc tcatgttggc
    18001 ttctgccgcc agttgctcga cgctcaggtt ttcggtgtat ttattctcaa ttcgtttcag
    18061 cacgcggcta atcagactga agtgagtctg gcgactgacc agcgccagta acgcgccgcc
    18121 gcaaggtccg gtcagcacgt agtacagaat ttcgcggatg atctgtttgc cgagaatacg
    18181 cgcatccagt ggtcgctcca tcacgtcgag taaccgctcc gccgcgcata aaatctcttc
    18241 tgataacgtg gcggagttaa tcccgctggc tgccatcgac ggctggaaat gctcatcttc
    18301 gccaatgtcc atcaacagtt cctgtaactg caaaatatcg acattgagac gcaaccctgc
    18361 cagcggcacc tctgacgtgg cataggtttc gcactcaaac ggcaacggca ccgtcagcag
    18421 caggtattca ttggcatcat aacgaaacac gcgttcattg atataaccga ttttatgccc
    18481 ggaaaagaga attatgatgc caggctcgta catcaccggt gtacgtgcga aaggcgtctc
    18541 gccatacaac aaacgcacat cgggcaacag ttctgacaaa ctattttctt tatttttcag
    18601 tttattaact ttatccgcca gcaagcgaca aatctcttca cgtttcatat cgcgtaattt
    18661 cttaggaata atgcggcaat ttgattgtgc gcaattttgt agcatttctc cagcactctg
    18721 gagaaatagg caagacattg gcagaaatga gcattgagag ccagggcgct ggcgatcaca
    18781 atgaaaaaca tcaggcagat cgttctctgc cctcatattg gcccagcaaa gggagcaagt
    18841 aatgaacaac tttaatctgc acaccccaac ccgcattctg tttggtaaag gcgcaatcgc
    18901 tggtttacgc gaacaaattc ctcacgatgc tcgcgtattg attacctacg gtggcggcag
    18961 cgtgaaaaaa accggcgttc tcgatcaagt tctggatgcc ctgaaaggca tggacgtgct
    19021 ggaatttggc ggtattgagc caaacccggc ttatgaaacg ctgatgaacg ccgtgaaact
    19081 ggttcgcgaa cagaaagtga ctttcctgct ggcggttggc ggcggttctg tactggacgg
    19141 caccaaattt atcgccgcag cggctaacta tccggaaaat atcgatccgt ggcacattct
    19201 gcaaacgggc ggtaaagaga ttaaaagcgc catcccgatg ggctgtgtgc tgacgctgcc
    19261 agcaaccggt tcagaatcca acgcaggcgc ggtgatctcc cgtaaaacca caggcgacaa
    19321 gcaggcgttc cattctgcac atgttcagcc ggtatttgcc gtgctcgatc cggtttatac
    19381 ctacaccctg ccgccgcgtc aggtggctaa cggcgtagtg gacgcctttg tacacaccgt
    19441 ggaacagtat gttaccaaac cggttgatgc caaaattcag gaccgtttcg cagaaggcat
    19501 tttgctgacg ctaatcgaag atggtccgaa agccctgaaa gagccagaaa actacgatgt
    19561 gcgcgccaac gtcatgtggg cggcgactca ggcgctgaac ggtttgattg gcactggcgt
    19621 accgcaggac tgggcaacgc atatgctggg ccacgaactg actgcgatgc acggtctgga
    19681 tcacgcgcaa acactggcta tcgtcctgcc tgcactgtgg aatgaaaaac gcgagaccaa
    19741 gcgcgctaag ctgctgcaat atgctgaacg cgtctggaac atcactgaag gttccgacga
    19801 tgagcgtatt gacgccgcga ttgccgcaac ccgcaatttc tttgagcaat taggcgtgcc
    19861 gacccacctc tccgactacg gtctggacgg cagctccatc ccggctttgc tgaaaaaact
    19921 ggaagagcac ggcatgaccc aactgggcga aaatcatgac attacgttgg atgtcagccg
    19981 ccgtatatac gaagccgccc gctaagcttt ttacgcctca aactttcgtt ttcgggcatt
    20041 tcgtccagac ttaagttcac aacacctcac cggagcctgc tccggtgagt tcatataaag
    20101 gaggaacgta tggctaatcc aaccgttatt aagctacagg atggcaatgt catgccccag
    20161 ctgggactgg gcgtctggca agcaagtaat gaggaagtaa tcaccgccat tcaaaaagcg
    20221 ttagaagtgg gttatcgctc gattgatacc gccgcggcct acaagaacga agaaggtgtc
    20281 ggcaaagccc tgaaaaatgc ctcagtcaac agagaagaac tgttcatcac cactaagctg
    20341 tggaacgacg accacaagcg cccccgcgaa gccctgctcg acagcctgaa aaaactccag
    20401 cttgattata tcgacctcta cttaatgcac tggcccgttc ccgctatcga ccattatgtc
    20461 gaagcatgga aaggcatgat cgaattgcaa aaagagggat taatcaaaag catcggcgtg
    20521 tgcaacttcc agatccatca cctgcaacgc ctgattgatg aaactggcgt gacgcctgtg
    20581 ataaaccaga tcgaacttca tccgctgatg caacaacgcc agctacacgc ttggaacgcg
    20641 acacacaaaa tccagaccga atcctggagc ccattagcgc aaggagggaa aggcgttttc
    20701 gatcagaaag tcattcgcga tctggcagat aaatacggca aaaccccggc gcagattgtt
    20761 atccgctggc atctggatag cggcctggtg gtgatcccga aatcggtcac accttcacgt
    20821 attgccgaaa actttgatgt ctgggatttc cgtctcgaca aagacgaact cggcaaaatt
    20881 gcaaaactcg atcagggcaa gcgtctcggt cccgatcctg accagttcgg cggctaacgt
    20941 gcaaattctc ccggtggcgg taatgttccg ctaccggact tttcagaaat catttattcc
    21001 cctcgcgtcc cgcccgttgt tactcttcct tgttcaggaa tgccaaatat aaggacatca
    21061 tcatgcagag ccggaagctc ttaaaagaac aactcatcta tatccgggat aaacgcaacg
    21121 gagaggtgaa aaacagatga aaataatact tctgttttta gcagccctgg caagttttac
    21181 cgtacacgcg cagcctcctt cacagaccgt agaacaaaca gtccggcaga tttatcagaa
    21241 ctataaatca gatgccagta cgccttattt cggtgaaacc ggagagcggg cgataacttc
    21301 agcgcgtatt caacaggcgc ttaccctgaa cgacaatctt acactaccgg gcaatattgg
    21361 ctggctggat tatgatccgg tttgtgattg tcaggatttt ggcgatctgg tgttagaaag
    21421 cgtagcgata acccaaactg acgtcgatca tgccgatgcc gttgtgcgct ttcgtatctt
    21481 taaagatgat aaagaaaaga ccacacagac actgaaaatg gtggcggaaa atggtcgttg
    21541 ggtcattgat gatattgtca gcaatcatgg cagcgtctta caagcagtta atagcgagaa
    21601 tgaaaaaacg ctggccgctt tagcttcgtt gcagaaagaa cagccagaat cctttgttgc
    21661 tgaactcttt gaacatattg ctgattatag ctggccgtgg acgtgggtgg tttccgactc
    21721 ttaccgccag gcggttaatg ccttctataa aaccaccttc aagacggcca ataatcccga
    21781 tgaagatatg caaatagaac ggcaatttat ttacgacaat ccgatctgtt ttggcgaaga
    21841 gtcgctattt tcacgcgttg atgaaattcg agtcctggag aaaaccgccg attccgcccg
    21901 cattcatgtt cgttttacgc tgaccaatgg caacaatgaa gagcaagagc tggttttaca
    21961 gcggcgcgaa ggtaagtggg aaatcgctga ttttatccgc ccgaacagcg gcagcctact
    22021 taagcagatt gaggcaaaaa ccgccgccag attaaagcaa tgagctgaat taaataacaa
    22081 ttagccggaa caataaataa aagggaacac tatatgaaaa cgattttcac cgtgggagct
    22141 gttgttctgg caacctgctt gctcagtggc tgcgtcaatg agcaaaaggt caatcaactg
    22201 gcgagcaatg tacaaacatt aaatgccaaa atcgcccggc ttgagcagga tatgaaagca
    22261 ctacgcccac aaatctatgc tgccaaatcc gaagctaaca gagccaatac gcgtcttgat
    22321 gcacaggact attttgattg cctgcgctgc ttgcgtatgt acgcagaatg ataaaaaaat
    22381 ccccggcagc atgtcagttg ccggggattt tttttaacgt ccaaccgccg ctttagggcg
    22441 tttcttcgca ccagcattca ccggacgaga ttgcgtagac gacgcttttt ttgccgtagc
    22501 aggcgtctga cgctgagtcg ccatcggcgt atgtttagtc aacgccggac gggtattgcg
    22561 gttctggcga cgagcttcac gcatctcttc aatggttggc gcaggcacta agcaatcgcg
    22621 acggctgcca atcagatgct ttttgcccat cgcttccagc gcctggcgga ttaacggcca
    22681 gtttgccgga tcgtggtaac gcaacaacgc tttatgcaaa cgacgctgtt tgtcgccctt
    22741 cggtacgaag acgccttcgc tcttataacc aatcttcgcc agcggttttt tcccggtgta
    22801 atacatggtg gttgagttcg ccagcggcga tgggtagaag ttctgtacct ggtcgagacg
    22861 gaaacgatgc tttttcagcc acagcgccaa attcaccata tcttcatcac gcgtaccggg
    22921 gtgcgcagag atgaaatacg ggatcagata ctgctcttta cctgcctgtt tcgagtaggt
    22981 atcgaacagc tctttaaagc ggtcatagct gcccatgccc ggcttcatca tcttcgacaa
    23041 cggcccttct tcggtatgtt ccggagcaat cttcagataa ccgccgacgt gatgggtcgc
    23101 caactctttg atatagcgcg gatcttccac ggcgatgtca taacgtacgc ctgaggcgat
    23161 aaggattttt ttaatgcctt tcagatcacg cgcacggcga tagaggttga tcgtcggttc
    23221 gtggttagtg tccatgtgcg gacaaatatc cggataaacg cacgacaaac ggcgacaagt
    23281 ttgttcagcg cgtggcgatt tgcagcgcaa catatacatg ttggcagttg gcccaccgag
    23341 atcggaaatc acgcccgtaa aacctggaac ggtgtcgcgg atcgcttcga tctcattaat
    23401 gatcgaatct tccgaacggc tctgaataat gcgcccttcg tgctcggtaa tagaacagaa
    23461 agaacagccg ccaaagcagc cgcgcataat gttgaccgaa aaacggatca tttcgtaagc
    23521 cggaatacgg gcattgccat aggccggatg tggcacgcgc ttgtacggca gcgcaaaaac
    23581 gctgtccatc tcttcggtag aaagcgggat agcaggcggg ttgatccaca catagcggtc
    23641 accgtgtttt tgcatcaatg cgcgggcgca gcctgggttg gtttcgtggt gcagaatgcg
    23701 cgaagcatgg gcgtacagca ctttatcgcc cttcactttc tcgaaagaag gcagcaatac
    23761 gtaggttttt tcccacggtt tctggcgcgg tggctgcacg attacagctt tcgcttcctg
    23821 ctttttcggc gcaacaggtt tgttatccgc acacggcaaa tcttcgccat acggatgcgg
    23881 gattgggtcg attttcccta gggtatcaag acgggtggaa tccacaccgc tccagccagg
    23941 cagcgcctct tttacgataa ttgcggtatt acgcacatcg cggatttcac taatcggctc
    24001 gcccatcgcc agacgatgcg ctacttccac cagcggacgt tcaccgttac caaacatcag
    24061 catgtcggct ttcgaatcta ccagaacgga acggcgcacg gtatcggacc agtaatcata
    24121 atgcgcggta cggcgcaggc tagcctcaat accgccgagg atcaccggca catctttcca
    24181 cgcctcttta caacgctggg tataaaccag cgtggcgcga tccggtcgct tacccgcgac
    24241 attatctggc gtgtaggcat catcatgacg taaacggcga tcggcggtat agcggttgat
    24301 catcgaatcc atgttgccag cagtaacacc aaaaaacaga ttcggtttac ccagacgcat
    24361 aaagtcgtct ttgctgctcc agtccggctg ggcgatgatc ccgacgcgaa agccctgcgc
    24421 ttccagcata cgaccgcaaa tcgccatccc gaagcttggg tgatcgacat acgcgtcgcc
    24481 agtaaccaaa atgatgtcgc agctatccca gccaagttga tccatctctt cacgagacat
    24541 cggcaaaaac ggtgccggtc caaaacaggc ggcccagtac tgcggccagg agaacaggtc
    24601 gcgatccggt tggatcaggg agatagagct cattttgctt ccagaaatga taaaaaaata
    24661 atcaaaggcc ggggattata agccggaacg aaagagaaat cgaaaggtat tccatactcg
    24721 ccctcctcgg gcgagtatga agattacggt accggattga ccaacagttg cccaatcgag
    24781 ccacggtccg ccatttccag cgtctgactg ttgaagtaga acgggaagtg cgcccaggaa
    24841 ggctgaccga aataaacaag cagctccact tgtccgtcaa cccaaacggt atctttccag
    24901 cctctgtctt ccgggaacgg catcgcgcca ttcacgttac ggatctggaa cattacgcct
    24961 tcaatatgga acgcttgcgg ctcgtccgcg cgtaccgtcc agcgttccca cgttccttgc
    25021 tgcgcggtga catcaatacg gttgacgtcc cacagctgtc cattaatacc cgggtcatca
    25081 cccagactga tatcgcgact gcgaattggc gaaccagcca tgatttcagt tggcagcaag
    25141 cgcatcggaa gactgtctgt gaccagcggc agaaggccgg ttgggcgcag cgttagcacc
    25201 aaggtagaaa ccagaatact ggatggctca aagaagccgc gaatacgatc aacaatgctc
    25261 gccgcttcac cacaggtgat cgacacttca tcgccgttgc tcatatccac cagaatctcg
    25321 cggcgctcgc ccggtgccag cgaaagttgc ttcaccgata caggagcagg caggaatccc
    25381 tgatcgccag aaatcacatg taacgggcga ccatcgctca tctgtagttg atagcgacga
    25441 gagttcgacg cgttcagcag tcgcaagcgc acccagccac gcgagacttc aacgtacggg
    25501 ctttgtacac cgttaaccag cagcgtatca ccaacaaagc cgccgcttcc cggttcgttg
    25561 tattctggcg taccaaagtt atccagccgt ttatcctgga taatgaccgg aaaatcatcc
    25621 acaccataat ggttggggat aggcagcgac ttgctgactt catcttccac cagccacatt
    25681 ccggcaaggc cgttatagac ctgctgagcc gtgcggttgg gagtattggc gtgataccac
    25741 agagtagctg cgttctggcg aataggcagt acgggtgccc agtcagcgtt tggcgacatc
    25801 atccgtgccg gaccgcccat cagcgggcct ggtacctgta gcccggccac cgtcattgag
    25861 acattttctg tcaggcggtt gctgtaaata agcttaacat cgtcgccctt ccagacgcgg
    25921 atagtcggcc ccaggtaacg accattgatt ccccagaccg acgcgcgtgt ccctggcgta
    25981 aatgaccagt gcgcacgttg tacagtcata aacagcggtt gcccacggcg agattcaagt
    26041 agcggcggaa cgggtagcgg ttgctgttgc ccggctgcgc tggccttcag gggaacagcg
    26101 cctgcacaaa gtgcaatccc cgatgcctga atgaactgac gccgactgag tgacatattt
    26161 gctccatgta aaactggcta aagtaatacc gtgcaggaaa gtgttccccg caaaaataca
    26221 gattgccgac ttaaactttt ccggcggctt cgcgttctgc gacttcttta tcgagctcgg
    26281 cgattttttg ttccattatc gaacgacaat gggcagccag ctcacgaacc tgatctttgc
    26341 catactgact gacgtcaatt ggcggcagca tttcgacaat caccagaccg ttgtgcagtc
    26401 gattaagatt aatcttattc gaagttgtag agacgcacac gggaataatc gggacgcccg
    26461 ccgcaattgc cgcgtgaaat gctccagtct tgaacggtag caggccgcga ccacggctgc
    26521 gggttccttc cgggaacatc cagatggaaa tacggcgttt tttgaaatga ttcacgactt
    26581 ccgcaatggt gccgtgagct ttagtgcgat tgtttctgtc gatcaataag ttgccggtta
    26641 accagtacaa ctgcccgaag aaggggatcc acagcaagct ctttttacct accgtcaccg
    26701 tcggcggttg cacaatgttc gatgctgtca ccatgtcata gttgttctgg tggttagcga
    26761 tatagatagc attgccgtag ctttcagcat ctgcaggttt gcggcactca actttcaggc
    26821 cgaacagtgg cgcaagacgg ccaaacatat gcccaaaggt ggccacatgt ttcgggttac
    26881 gcgggctgaa aaggcagtaa atggagccga atacacagac taagatgctg taaatcacgg
    26941 taataataag acgaaagata tatagcataa caccctctga aggtctgaga gactggcatt
    27001 gtacgtcacc tgaaatcagg ttagggcatt gttaaaagcc gctctctgga aaagcgtatt
    27061 gttaatcgca acgcatgaat aaacaacggt ttcaagggaa tttttatcgt ttccgggggt
    27121 tgaatacgct gccggatgac gacttaacgt ttcatccggc gttccttgct agcgggagga
    27181 aacagcgccc tccccggcat attactcttc gctatcaccg ctgctggcac ggcgaggaga
    27241 gtcgatctca acacgatcga tacgctgcaa accgcgcatc aacgtaccgc ggcgtccacg
    27301 ttcgccagtg actttctgta gctcttccgg acgcagttta attttgcgtt tcccaacatg
    27361 aatggtcagc gtgctttgcg gcggcagaac gtacagttgc gccagaccat cctcgccacg
    27421 cgcggcttct gccgatggaa tgttgataat cttgttgcct ttgcccttcg acagctgcgg
    27481 cagatcgctt accgggaaca tcaacatacg gcctgcctga gtgattgcca gcagcatatc
    27541 ggaagcatct tcaatcacca ccggcggcat aacatgggca ttttccggta aggtgatcaa
    27601 agccttaccc gcacggttac gcgctaccag atcgttaaag gtgcagacga aaccgtaacc
    27661 cgcatcggaa gccatcagca gtttctgatc gtcgctttcc atcagcatat ggtcaacggt
    27721 cgccccaggc ggcaacgtta atttgccggt gagtggttcg ccctgaccac gcgccgacgg
    27781 cagcgtaatc gggtcgatgg catagctacg accggtggaa tcaacaaaca ctaccggttg
    27841 gttactctta cctttcaccg ccgctttgaa gctatcaccc gctttataat tcaggcccgg
    27901 cgcgtcgata tcatggcctt tagcgctgcg tacccagccc atctgcgaca gcacaatggt
    27961 gacaggttca gacggcagca tgtcgtgctc gctcatcgct ttcgcttctt cgcgttcctg
    28021 caacggcgaa cgacgatcgt caccgtaggc ttgcgcgtct gcctgcagtt ctttcttcag
    28081 caggttattc attttacgct cggaagccaa aatgccctgc aactggtcgc gctctttttc
    28141 cagctcactc tgctcaccgc gaatcttcat ctcttccagt ttggcaagat gacgcagttt
    28201 cagttcgagg atcgcttccg cctgggtttc cgtaaggcca aaccgcgaca tcagcgccgg
    28261 tttcggttca tcttcgttac gaatgatctc aatcacttcg tcgatgttga gaaacgccac
    28321 cagcaaacct tccaggatat gcaggcgctt gaggactttc tccagacgat agttcagtcg
    28381 gcggcgcacg gtatcgcgac ggaacaccag ccattcggag aggatttcca gcaggttttt
    28441 caccgccgga cgaccatcca gaccgatcat attgaggtta atgcgatagc tcttttccag
    28501 atcggtggta gcgaagaggt ggttcatcac ctgatccata tccacgcggt tggaacgcgg
    28561 cacaatcacc agacgggtcg ggttctcgtg gtcagattca tcgcgcagat cgtcaaccat
    28621 cggcagcttt ttgttgcgca tttgcgcagc aatttgctcc agtacgcgcg cacctgaaac
    28681 ctgatgcggt aatgcgctga taaccaccgc gccatcttct ttcttccaca ccgcgcgcat
    28741 acgcactgaa ccacgtccgt tctcgtagat tttacgaatc tcggcgcgcg aagtgataat
    28801 ttccgcttca gtcggataat ccggcccctg cacgatatcc agcagctgat cgagcgtagt
    28861 tttcggctgg tcgattaatg cgattgccgc ctgagctact tcacgcaggt tatgcggtgg
    28921 aatatcggtc gccatgccga cggcaatacc ggtggtgccg ttaagcaaaa tgtttggcag
    28981 acgggcaggt agcattttcg gctcctgcaa agtgccgtcg aagtttggca cccagtcagc
    29041 cgtcccctgc cccagctcgc tcaatagcag ctcggaatat ttcgacaacc gggattcggt
    29101 gtaacgcatt gccgcgaacg atttcggatc gtccggcgcg ccccagttcc cctgaccatc
    29161 aaccagcgga taacggtaag agaacggctg cgccatcagg accatcgctt cataacaggc
    29221 gctatcgccg tgcggatggt atttacccag tacgtcaccg acggtacggg ccgatttttt
    29281 aaatttggcg ctggcattca ggcccagttc agacatcgca tacacaatgc ggcgctgaac
    29341 aggtttcaga ccatcaccaa taaacggcaa cgcacggtcc atgatcacgt acatggagta
    29401 gtttaagtag gcgttttccg taaattcatg tagcgcaagg cgctctgcca tatcgctcat
    29461 taattctgat tcctcaactt attcgccagc cttcaaaggg gcaatattgc cggagatact
    29521 acattatctg ccgcgtcgag tcacaggaga tcagcgtgaa tagcgccgcc ggacgtgctc
    29581 cgacggcgat aaaggcatca atgtgccttg atatacaact ctttgctgta gacttgatcg
    29641 gcatagaggg cagcacaagg ttgcgcccat gccacaccac tcggcatggg ggtccgctcc
    29701 gggcgattcg agaagttgag gttatgcgag tccagggaag cctaaagcat caaagtagtc
    29761 gactgtaagt atacggttca gcccagttta gctgtgtctc caccagtgcc ccgcgcctcc
    29821 tgccataagt gcggcttgct catggatatc cccgagacgg cttacggcgc accggataat
    29881 aaatttcgcg tatacatggt attccttacc ggagaaatta taagcccaac aacaatacgc
    29941 ctgtgacatt gttcgctcaa acttatcgcg agtgatttgg ctcacactcc ggtaaagatt
    30001 ccccatcacg ccaacggcac ataaacatcc gtttccagtc gctgctctgt caccccttcg
    30061 gcaagattgg tgtagtgaaa cagaatcggt gctttacgca ttttctcgcc gcttgcaggc
    30121 agccagtggc gaataatgcc ccatatcgtg tgtgaaatat cgtccagctc gccaacgtgg
    30181 cgggccacgg cataacgtcc accggtaagt tcaccattgc tcacaccata acgattatcg
    30241 ggaatcagtt cgctaacgct gccgcagata tcaaagcgaa acgcttccgg tgcggtggtg
    30301 gcaggggcgt cccaggcgac gccaaaagtc tggctttggt taacaggaga aagccccgtc
    30361 tctttacgcc acatgataaa tttcgccaca ctgtagttga gcaactcagg gctacagcgg
    30421 tgcggcaaca tcgccacagg gattgatggg aaatcgataa tgtttacatc cagtgtcagg
    30481 tttgtcattt tgcctccctg cgaaattatt cccgggaacg cgtcccggga gcggtaacaa
    30541 ttacggattt actttgcgga tctgtttgac gtcaatttca acagaattcc agtctttatc
    30601 gacttcaccc tgaatctcaa ccgtatcttt cggcgtcacc gtcacaccgt tccagcgttt
    30661 gtgatcgata tcaacattga tagtaccgct ggcatcttta aacacgtaga gatcgtcaga
    30721 gatgcgttca acgatattgc cgcgcagggt tacccaggtg tcgtcacgca gggattttgc
    30781 gctttctaca gtcgttacgc tgccgttcgg cccctgaaat cctccggcct gactttgcgt
    30841 taccgatggg ccagaaaaac cgccctgctc tgctgccatc accggtgcac tgcacagggc
    30901 cattactgcg attactgctg cgaatttttt catgtttatt actcccttta atgtctgttt
    30961 ccgagcattt aacaagatag tccttaacaa cttcttaagg gaaaaaaata aaatttaatg
    31021 ctgtacagag cgcgttacaa cacggtttac tggcagcaaa tacggttatc gcagggatga
    31081 aaaaatgcga attttactga tagaagatga catgctgatt ggtgacggca tcaaaactgg
    31141 ccttagtaaa atgggtttta gtgtcgactg gtttacacaa ggtcgtcagg gaaaagaggc
    31201 gctttatagc gcaccttatg atgcggtgat cctggattta accttaccag gcatggatgg
    31261 tcgcgatatt ttgcgcgaat ggcgagagaa aggtcagcgt gagccggtac tgaacctgac
    31321 cgcgcgtgat gcgttagcgg aacgtgtaga agggctgcgt ctgggagctg acgattatct
    31381 gtgtaaacct tttgcgttga tagaagtcgc cgccaggctg gaggctctga tgcgccgaac
    31441 caacggccag gccagcaacg agctgcgcca cggtaacgtc atgctcgacc ccggcaaacg
    31501 tatcgccacg ctggctggcg aacccttaac actgaaacca aaagaatttg ccctgctgga
    31561 attactgatg cgtaacgctg gtcgggtact gccgcgcaaa ctgattgaag agaaactgta
    31621 tacctgggac gaagaggtca ccagtaatgc cgttgaagtg catgtgcatc atctgcgacg
    31681 caaactcggt agtgatttta ttcgtaccgt gcatggtatt ggctacacat taggtgagaa
    31741 atgaaattta cccaacgtct tagtctgcgc gtcaggctga cgctaatctt tttaattctg
    31801 gcctcggtga cctggctgct ttccagcttt gtcgcctgga aacaaacaac ggataacgtc
    31861 gatgaattgt tcgacaccca actgatgctg tttgccaaac ggttaagtac gctcgatctc
    31921 aacgaaatca acgcggcgga tcgcatggca cagacgccaa ataaattaaa acacggtcat
    31981 gttgatgacg atgcgctgac ctttgccatc tttacccacg acggcagaat ggtccttaat
    32041 gatggcgata acggagaaga tattccctat agctatcaac gggaaggttt tgctgacggg
    32101 caactggtcg gtgaagacga tccttggcgt tttgtctgga tgacctcacc tgatggcaaa
    32161 tatcgcatcg ttgttggcca ggaatgggaa taccgtgaag acatggcgct ggcgattgtt
    32221 gccgggcaat tgatcccgtg gctggtcgca ctgccgatta tgttaatcat catgatggta
    32281 ctactgggtc gtgaactcgc gccgctgaac aaactggcgc tggcactacg tatgcgtgac
    32341 cctgactcgg aaaaaccact aaacgcgact ggcgtaccca gcgaagtgcg gccactggtt
    32401 gagtcgctaa atcaactgtt cgcccgcaca catgcgatga tggttcgaga acgacgcttt
    32461 acctccgatg cagctcacga actacgtagc ccgttaacag cgctgaaagt gcaaaccgaa
    32521 gttgcgcagc tctctgacga tgatccgcag gcgcggaaaa aagcactgct ccaattacat
    32581 tccgggatcg atcgcgctac tcgtctggtt gatcaactgc tcacgctatc gcggctggac
    32641 tcactggata accttcagga cgtcgcggag atcccgcttg aagatctcct acaatcgtcg
    32701 gtgatggata tttaccacac ggcgcagcag gcgaaaattg acgtgcgact gacactcaat
    32761 gcccacggca tcaaacgcac cggacaaccg ctattgctaa gtttgttggt gcgaaatttg
    32821 ctggataacg gccccggtgt gacaccagag gcactggcgc gaattggcga acgcttctat
    32881 cgcccacccg gacaaaccgc taccggcagt ggacttgggc tatcgattgt ccagcgaatc
    32941 gccaagctgc atgacatgaa tgttgagttt gggaatgcgg aacaaggtgg atttgaggcg
    33001 aaggtaagct ggtaacttct ctgtctatga gagccgttaa aacgactctc atagatttta
    33061 ctaatagcaa aatataaacc gtccccaaaa aagccaccaa ccacaaacca gacaaataac
    33121 gagcgagaat acatatagcc aaaactcgaa catttcccgc ataaagagtt ttcttaagat
    33181 aagaataata agtggcgtaa gaagaaaaaa tgctgcaatc attgcttgca atatacgatc
    33241 ttcgcctgaa acggaaggta tactgcaaat attgcctccc gactcaagca tccagtcata
    33301 cttattgaaa gcaaaaaaag aatctattaa ccaataagta agcattatat atagcaatgc
    33361 ttcaaatatt gttttaattt tctgctttaa catatatcag tacgctcata aaaatccatt
    33421 tataattatc aacatgacac gttgatattt tcatctttta cttattcaaa gccatatttt
    33481 caatccccaa aaaaaccgaa ccaaacgtgt aaattattcc ggtgcgagta agaattatga
    33541 ggaatggcta tcagtattgt cattttcaga aaatatttat cctgcatcgg tgagtcagag
    33601 ctagatcaga cttttgctaa attcgcaaaa gactttgcac attttgctaa tttcacagta
    33661 ccgctctgtg acgtactata gtcgacaaac gtctcacctt gagattaaaa aatgagcaac
    33721 atcctgatta tcaacggcgc gaaaaaattc gcccactcca atggtcaact gaacgacacc
    33781 ctgaccgaag tcgcggatgg cacactgcgc gacctcgggc atgatgtccg catcgttcgc
    33841 gccgacagcg actacaatgt caaagcggaa gtacaaaact ttctctgggc tgatgtggtg
    33901 atctggcaga tgccaggctg gtggatgggc gcgccgtgga cagtgaaaaa atatattgat
    33961 gatgtattca ccgaaggtca cgggactctg tatgccagcg atggtcgtac ccgcaaagat
    34021 ccgtcgaaaa aatacggttc cggcagcctg gtacaggaca aaaaatatat gctttctcta
    34081 acctggaacg caccaatgga agccttcacc gaaaaagatc agttcttcca cggcgttggc
    34141 gttgacggtg tgtatctgcc gttccataaa gcaaaccaat tcctcggtat ggaaccgctg
    34201 ccgacattta tcgctaatga cgtgataaaa atgcctgatg ttccccgcta tactgaagaa
    34261 tatcgcaagc atcttgtgga aatttttggt taactagagc tcaggcttta gaaggagtta
    34321 accatgctta ccgtaatcgc agaaatccgt actcgtcctg gtcaacatca ccgtcaggcg
    34381 gtattggatc agtttgctaa aatcgttcca accgtactga aagaagaagg ttgccacggc
    34441 tatgcgccaa tggtggattg cgctgctggc gtgagtttcc agtctatggc accggattct
    34501 atcgtgatga ttgagcagtg ggaaagcatc gcgcaccttg aagcgcatct gcaaaccccg
    34561 cacatgaagg cgtacagcga agccgtaaaa ggcgacgtgc tggagatgaa tatccgtatt
    34621 ctgcagccag ggatttaatc ctgccttgtt tgcccggcca tcctgaccgg gcaatgttct
    34681 ttcctttaaa cctcaatctc cgccatgtcg cctttctctt gcaaccagtt gcggcgatct
    34741 tccgagcgtt tcttcgccag cagcatatcc atcatcgcgt cagtacgctg atcgtcttca
    34801 tcatcgatag tcaactgcac cagacggcga gtgttcggat caagcgtggt ttcacgcaat
    34861 tgcatcgggt tcatttcccc cagaccttta aaacgctgga cgttcggctt gcctttcttg
    34921 cgttttaatt gctcaagtac gccctctttc tcttcttccg tcagcgcgta ataaacctct
    34981 ttcccgagat caatacggta gagcggtggc agtgcgacgt aaacgtgacc gtgtttcacc
    35041 aacgcgcgga agtgttttac gaacaaagcg cagagcagcg tggcaatgtg cagaccatca
    35101 gagtccgcat ccgcgaggat acagattttg ccataacgaa gctggctcag atcgtcgctg
    35161 tcaggatcga taccgatcgc taccgaaata tcatgcactt cctgcgaggc cagcacttcg
    35221 tcggaagaaa cttcccaggt gttaaggatc ttacctttca gcggcatgat cgcctgatat
    35281 tcacgatcgc gcgcctgctt ggcagatccg cctgcggagt caccttccac gaggaacagt
    35341 tcggtacggt taaggccctg cgcggtacaa tcagccagtt tgccaggcag cgccgggccg
    35401 ctggtcagct ttttacgcac cactttttta gccgcacgca tacgacgctg ggcgctggaa
    35461 atcgccatct ctgccagcaa ctccgccgcc tgaacgttct ggttcagcca caggatgaag
    35521 gcatctttca ccacgcccga aacgaatgcc gcacactgac gcgaagagag acgctctttg
    35581 gtctgcccgg caaactgcgg atcctgcatt ttaaccgaca gaacataggc gcagcgatcc
    35641 cagatatctt ccgccgacag cttcacgccg cgcggcagaa tgttgcggta ttcacagaac
    35701 tcacgcatcg cgtccaacag gccctgacgc agaccattaa catgggtacc gccctgcatc
    35761 gttgggataa ggttgacgta gctttcggtc agcagttcac cgccttccgg cagccacagt
    35821 agcgcccagt ccacagcttc agtatcgcca gcgaaattac cgataaacgg tttttctggc
    35881 agcgtcggta aaccgtttac cgcttccgcc aggtaatcat tcagaccgtc ctgatagcac
    35941 cagcgctgtt cggtgttgtt gatctcatct ttaaaggtaa tctcaacgcc ggggcacagt
    36001 actgctttgg ccttcagcac atgcgtcagg cgtgaaacag aaaaacgcgg gctgtcaaag
    36061 aaggtttcat ccggccagaa gtgcacgctg gtaccggtat tgcgtttacc gcaagtgccg
    36121 acaacctgta aatcctgcac cttttcgcca ttttcaaagg cgatgttata aacctgacca
    36181 tcgcggcgca cgttaacttc tacgcgcttc gacaaggcgt taactaccga aatccccacg
    36241 ccatgcaggc cgccagagaa ctggtaattt ttgttagaga atttaccgcc tgcatgcaga
    36301 cggcaaagaa tcagttcaac cgccggtact ccttcttccg ggtgaatatc caccggcatc
    36361 ccgcgcccat cgtcaatcac ttctaacgac tggtcagcat gtaagattac atccacgcgt
    36421 tttgcgtgac ccgccagtgc ttcatcgaca ctgttatcaa taacttcttg ccccaaatgg
    36481 ttagggcgag tggtatcggt atacatcccc ggacggcggc gaaccggctc aagcccggtg
    36541 agtacctcaa tggcatcagc gttataagtt tgcgtcatgg tttaagttag caattcgagt
    36601 tgatcgtcag agatggtgca gaccaagaaa atcgacgatc gggttgaaat aatcttcgaa
    36661 gcccgtgaat gcgtggttgc cgccttctat gacagtctgg cggcaggaag catagtacgc
    36721 caccgcctgg cggtaatcca gcacttcatc tcccgtctgt tgcagcagcc agatcaaatc
    36781 cggcgcttcc agcgggtcaa tctgcatgac tttgagatcg taaatatggc gtgactctag
    36841 cacatattgc tgcccggtgt aggggttctc gttctgaccg agatagtccg tcagcaattc
    36901 aaacgggcgt accgccgggt ttaccaccac tgcgggcagc ataaaacatt gcgacaacca
    36961 ggtggcgtaa tatcccccca gtgacgaacc gacaataccc agcgaatcgc cgccatgttc
    37021 caggacaatg gattccagca gttctgccgc gtcggaagga tacggcggca actgcggaat
    37081 gatcatctca acgtcagggt gatgttccgc cagccagttt tttaacaagc tcgcttttgc
    37141 agagcgcggc gagctgttga aaccgtgtaa ataaagaagc gtagacatca gtagccttct
    37201 gaagcggtat caggttggaa acgtgtgtcc gccaggcgat gcacctcggt ggtcagcgtg
    37261 ccatcagcat gtaactcgag agtacgccag ccgggcgcga tagtatccag cgtaaagttg
    37321 gaacagtgcg gcttaaactg cacacaggtc gacggcgttg ccagcaggcg gcgaccgttc
    37381 cagtcgagat ccagctcctg atgaatatga ccgcacagca agtatttgac gtgcggaaac
    37441 ttcgccagca cggtatccag ttcgcccgcg ttacgcagac tgtgttgatc gagccaacta
    37501 caacccgcag gtagcggatg atgatgcagc agcagcaacg tatggcgttc tggcgcatcg
    37561 gccagtttac gttccagcca ctcaagctga aactcgctca gctcaccgtg cggcacgcca
    37621 aacacctggc tatccagcaa caggatttgc cattgctcac caataaacac ccgcttcgcc
    37681 ggggagatac ccgcatcctg taacgcgctg tacatcgcgg gctggaaatc gtggttgccc
    37741 ggcagccaga cgcagggcgc acgaaaactt gcgatgcctt cagcgaaatg ctgataggcc
    37801 gcagaggatt gatcctgcgc taaatcacct gtcgcgacaa tcaggtcgaa ttcgtgctgg
    37861 tgtggacgaa tcgcctccag caccgcctgg tagctttccc aggtgtttac ccctaacagg
    37921 gcttcgtgct tttgtgcaaa caggtgagta tcggtaattt gtaaaatcct tcctctggcc
    37981 tcaccagcca gaggaagggt taacaggctt tccaaatggt gtccttaggt ttcacgacgc
    38041 tagtaaaccg gaatcgccat cgctccatgt gctaaacagt atcgcaacca gtccgctaaa
    38101 aactggttaa tttgatgctt ttcgtcgcgt tgatgcaact ttttattagg ataatcatac
    38161 cgcgctttga agcgaaaaat ctgctggctt gaacacactt cagccaccat cgcgtcatga
    38221 tacagacgca ccgtcattga cggaaggctc cagtaactga tcgcgggcgc agtctgttct
    38281 attgtcacca gggtagtgta tcgggtcgat tccacaatcg tcagccgata ttgtgcgttt
    38341 gccacctgat agcttacagt ttcgccgggt gcgtcattgc gcggtaacaa acggcgcaat
    38401 tgtgaaaagt tcatctcgca caggcgcatc atttcaggaa agtcaggtgt gtaacgcttc
    38461 atttatgccc actcattttt taacgcttga tgatgcagct gcagccattg caaagcgatg
    38521 accgacgctg cgttgtcgat tttcccctct tctacccact ggtatgcctg ttcccggctt
    38581 accacatgaa cgcgaatatc ttcgttttca tcagccagac catgaatacc gcttgcggtc
    38641 gtggcgtcca cttcgcccac cataattgac gaacgctcac tggtgccccc cgggcttgcc
    38701 aggaaactta acaccggttt ggtccgtttg actatcagtc ccgcctcttc aatcgcttcg
    38761 cgacgggcaa catcttccac actttcaccc tcttcaatca tcccggcaac catctccagt
    38821 agccaggggg tttcgctggt gtcgtacgcg gcaatccgaa tctgctcaat cagcacaact
    38881 tcatcacgca ctgggtcaaa gggtagcaag actgcggcgt gaccgcgctc aaaaatttcc
    38941 cgccgtacct catgactcat ttgcccgttg aatagacgat gacgaaatct ataaagatct
    39001 aatgaaaaaa agccgcgata aagtgtttct cgtgcaataa tttctacatc gtttttgcca
    39061 aatgtaacgg gcaggttgtc tggcttaagc attgttaatg tcctagcact aatagtgaat
    39121 taaatgtgaa tttcagcgac gtttgactgc cgtttgagca gtcatatgtt aaattgaggc
    39181 acattaacgc cctatggcac gtaacgccaa ccttttgcgg tagcggcttc tgctagaatc
    39241 cgcaataatt ttacagtttg atcgcgctaa atactgcttc accacaagga atgcaaatga
    39301 agaaattgct ccccattctt atcggcctga gcctttctgg gttcagttcg ttgagccagg
    39361 ccgagaacct gatgcaagtt tatcagcaag cacgccttag taacccggaa ttgcgtaagt
    39421 ctgccgccga tcgtgatgct gcctttgaaa aaattaatga agcgcgcagt ccattactgc
    39481 cacagctagg tttaggtgca gattacacct atagcaacgg ctaccgcgac gcgaacggca
    39541 tcaactcgaa cgcgaccagt gcgtccctgc agttaactca atccattttt gatatgtcga
    39601 aatggcgtgc gttaacgctg caggaaaaag cagcagggat tcaggacgtc acatatcaga
    39661 ccgatcagca aaccttgatc ctcaacaccg cgaccgctta tttcaacgtg ttgaatgcta
    39721 ttgacgttct ttcctataca caggcacaaa aagaagcgat ctaccgtcaa ttagatcaaa
    39781 ccacccaacg ttttaacgtg ggcctggtag cgatcaccga cgtgcagaac gcccgcgcgc
    39841 agtacgatac cgtgctggcg aacgaagtga ccgcacgtaa taaccttgat aacgcggtag
    39901 agcagctgcg ccagatcacc ggtaactact atccggaact ggcggcgctg aatgtcgaaa
    39961 actttaaaac cgacaaacca cagccggtta acgcgctgct gaaagaagcc gaaaaacgca
    40021 acctgtcgct gttacaggca cgcttgagcc aggacctgga gcgcgagcaa attcgccagg
    40081 cgcaggatgg tcacttaccg actctggatt taacggcttc taccgggatt tctgacacct
    40141 cttatagcgg ttcgaaaacc cgtggtgccg ctggtaccca gtatgacgat agcaatatgg
    40201 gccagaacaa agttggcctg agcttctcgc tgccgattta tcagggcgga atggttaact
    40261 cgcaggtgaa acaggcacag tacaactttg ttggtgccag tgagcaactg gaaagcgcac
    40321 atcgtagcgt cgtgcaaacc gtacgttcct ccttcaacaa cattaatgct tctatcagta
    40381 gtattaacgc ctacaaacaa gccgtagttt ccgcgcaaag ctcattagac gcgatggaag
    40441 cgggctactc ggtcggtacg cgtaccattg ttgatgtgtt ggatgcgacc accacgctgt
    40501 acaacgccaa gcaagagctg gcgaatgcgc gttataacta cctgattaat cagctgaata
    40561 ttaagtcagc cctgggtacg ttgaacgagc aggatttgct ggcactgaac aatgcgctga
    40621 gcaaaccggt ttccactaat ccggaaaacg ttgccccgca aacgccggaa cagaatgcta
    40681 ttgctgatgg ttatgcgcct gatagcccgg cacccgtcgt tcagcaaaca tccgcacgca
    40741 ctaccaccag taacggtcat aaccctttcc gtaactgatg acgacgacgg ggcttcggcc
    40801 ccgtctgaac gtaaggcaac gtaaagatac gggttatctg ccgcattctt cccccttctc
    40861 gcttcaattt cgaccagcca tcctctattc tgatgggtat ttaccactgg tcccggaaga
    40921 caaaaatgaa acggacaaaa tccatacgcc acgcatcgtt ccgcaaaaac tggagcgcac
    40981 gccatctgac accagtcgct ctcgcggttg ccactgtttt tatgctggct ggctgtgaaa
    41041 agagtgatga aacagtgtct ctctatcaaa atgctgacga ctgttcagct gcaaacccag
    41101 gcaaaagcgc cgaatgtacc accgcgtaca acaatgcgct gaaagaagcc gaacgtactg
    41161 cgccgaaata cgccacccgt gaagactgtg ttgctgaatt tggtgaaggt cagtgtcagc
    41221 aagcaccagc ccaggctggc atggcaccag aaaaccaggc gcaggctcag caatccagcg
    41281 ggagtttctg gatgccgctg atggccggtt acatgatggg gcgtctgatg ggcggcggcg
    41341 cgggatttgc acagcagccg ctgttctcct cgaaaaaccc ggccagtccg gcttacggta
    41401 aatataccga cgcgacgggt aaaaactatg gcgcagccca gccaggccgc accatgaccg
    41461 taccgaagac ggcaatggca ccaaaaccgg cgaccaccac taccgttacc cgtggcggtt
    41521 ttggtgaatc tgttgccaaa caaagcacta tgcagcgtag cgcaaccggc acctcttctc
    41581 gttcaatggg tggctgatac cgatggaaag agtcagtatt accgagcgcc cggactggcg
    41641 tgagaaagcc cacgaatacg gtttcaattt tcacaccatg tacggcgagc cgtactggtg
    41701 tgaagatgct tactacaagt tgaccctcgc ccaggttgaa aagctggaag aagtcaccgc
    41761 cgaactgcac cagatgtgcc tgaaagtggt ggaaaaagtg atcgccagcg atgagctgat
    41821 gaccaaattc cgcataccaa aacacacctg gagttttgtg cgtcaatcat ggctgactca
    41881 ccagccatcg ctttattcgc gtcttgatct ggcgtgggat ggcactggcg aacctaaact
    41941 tctggaaaat aacgccgata cgccaacgtc actatacgag gcggcgttct ttcagtggat
    42001 ctggctggaa gatcagctta acgccggtaa cttgccggag ggcagcgacc agtttaacag
    42061 tctgcaagaa aaactgatcg atcgcttcgt tgagctgcgt gaacagtatg gcttccagtt
    42121 gctgcatctc acctgctgtc gcgacacggt ggaagatcgc ggaaccattc agtatttgca
    42181 ggactgcgca accgaagctg aaatcgccac cgagttcctc tacatcgacg agataggttt
    42241 aggcgaaaaa ggccagttca cggatttaca ggatcaggtg atttctaacc tgttcaagct
    42301 ttatccgtgg gaatttatgc tgcgcgaaat gttctccacc aagctggaag atgctggcgt
    42361 acgctggctg gaaccggcgt ggaagagcat tatctccaac aaggcacttc taccgctact
    42421 gtgggagatg ttcccgaatc acccgaacct gctgcccgct tattttgcgg aagatgatca
    42481 tccgcaaatg gaaaaatatg tggttaaacc gatcttctcc cgtgaaggcg caaacgtgtc
    42541 gatcattgag aacggcaaaa ccattgaagc agcggaaggt ccgtatggcg aagaagggat
    42601 gattgttcag caattccacc cgttaccgaa attcggcgac agctatatgc tgattggtag
    42661 ctggctggtg aacgatcaac ctgccggaat tggcattcgt gaagaccgtg cgttgatcac
    42721 ccaggatatg tctcggtttt atccacatat ttttgttgaa taagccacga taccggatgg
    42781 cactcgccat ccggtaattg ttagcctatc tgcaccgaca gcatacttaa actgcccatt
    42841 tctataccat cgaccggaat ggtaattggc tcctgcccat cccacacacc taacacatac
    42901 aacaacggca gatagtgctc tggcgttggg ttcaataacg cgccaccttc atggtcgaga
    42961 taattcacca gaggatgctg ctctaccggc ccttgccacg tcagattcgc tttcacatac
    43021 tcattaaacg acgtcgccca cggatacggt gaactatcac cgtgccactt cactgtgcgc
    43081 aggttatgca ccacgttacc gctggcgacc aacattattc cttcatcacg cagcgccgcg
    43141 agtttgcgcc ccatttcgaa atgccaggcg gcaggtttgc tactgtcgat actcaactgc
    43201 accataggga tatcggcgtc aggatacatc ttaatcagca cgccccacga gccgtggtca
    43261 aagccccagg cttctttatc cagcgtcacc gggatcggcg ctaacagctc aaccagacgc
    43321 tgtgccagcg caggcgaacc cggagcagga taatgcgtat cgtacagcgc ctgcgggaag
    43381 ccaccaaagt catgaatcgt gggcggcgtc tccatcgcgg tcactcctgt tccacgggta
    43441 aaccagtgag ccgaaaccac cacaatcgct tgcgggcgtg gcaatgtcat ccccaacttc
    43501 tgccagctgc gggtatacaa attatcttcc agcacgttca tcggactacc gtgacctaaa
    43561 aacaatgctg gcatacgtgt tgaagacatg atgatatcct taactaaagg tgtcattttg
    43621 atatcctcac aatacgcttg ttcggcggag taagaacccg gataacaatg atgatgatca
    43681 tcagttattt tgacgatctg cctgaaggtg aagatttata aggagttgtc gatgtcagta
    43741 cctctcattc tgaccatact ggcgggggca gccacgttta ttggcgcgtt tctcggcgtt
    43801 ctcggacaaa aaccctcgaa ccgcttactg gcgttttcgc taggttttgc ggcggggatc
    43861 atgttgctca tctcattaat ggaaatgctt cctgccgcac tagcagctga aggaatgtcg
    43921 ccggtgttgg gttatggaat gtttatcttc ggtctgcttg gctactttgg cctggaccgc
    43981 atgttgccgc atgcgcatcc gcaggattta atgcaaaaat cggtgcagct gttgccaaaa
    44041 tcgatcaagc gcacagccat tctgctcact ctcggcatca gtctgcataa cttcccggaa
    44101 gggattgcca cctttgtcac ggcgagcagc aacctggagc tgggatttgg catcgcactg
    44161 gccgtcgcgt tgcacaatat ccctgaaggt ctggcagtgg caggcccggt ttatgcggca
    44221 acggggtcta aacgcaccgc gattctgtgg gcggggattt ctggactagc agaaattctt
    44281 ggtggtgtgc tggcgtggtt aatcctcggt agcatgattt ccccggtggt catggcggca
    44341 atcatggcgg cggttgcagg aattatggtg gcgctctcgg ttgatgaatt aatgccgctc
    44401 gccaaagaga ttgaccctaa taataacccc agctatggcg tactatgtgg aatgtcagtg
    44461 atgggattca gtttagtgct gctacaaacg gcgggaattg gttaaaaaca acatcagaag
    44521 ttgtccagca catggacaac ttctgccagc ttcacgaaag ccttattcta ccttttcggc
    44581 tgtctcttct ctcgtactgt ttaagtattt gttccgcgct tcaagcagtg cgtctctgtc
    44641 ctggcgaaac gcccgacaat aacgtttcaa atgcaggaaa taacccacca caaccagagc
    44701 aatcaataca atccagtacc aggcgataaa catcctttta cccttaatat ttaacaatac
    44761 taaacattta gcgcataaat ttcacatatc ctttttcgga tatattcata tggtcgcata
    44821 ataacggaac aattatatga ttttgatcat aatgaaaaag aatgcctgat attaatgaaa
    44881 ttttctcata gaaagagaag cggaattaac atcttgcata gcagggcaaa ataaaaaccg
    44941 aagccagcta caacttcggt ttcttgatta aggcagtaat taagcagcgg ttttcagctg
    45001 gctttacgct catgtgcctg acggtatgcc accaggtctt caatagtcac gagcgccata
    45061 ttgtgtttat tggcaaactc aatgcactct ggtgcacgcg ccatcgtgcc atcgtcatta
    45121 gtcagctcac acagcacacc agccggttta aagcctgcca gcgtcatcaa atcaatagtc
    45181 gcttcagtat gaccgccacg cgtcagtaca ccacctgcct gcgcgcgaag tgggaaaacg
    45241 tggcccgggc gattcagatc agaaggtttt gcgccatcgg caatcgctgc gcgaacggtc
    45301 gtaatacgat cagcggcaga aacaccggta gtcacacctt cagccgcttc aatggtcacg
    45361 gtaaaaccgg tgccataggc gctggtgtta ttttctacca tcattggcag atcaagttgt
    45421 ttacggcgat cttcagtgat gcacaggcaa acaataccgc taccgtggcg aatggtcagc
    45481 gccatctgct caacggtcat ggtttctgcc gggaagatca tatcgccttc gttttcacgg
    45541 tcttcatcat caagcaccat tacaccgcgt ccttcacgca gcgcagccag tgcattttca
    45601 acacgttcga aaggcgtacc aaaagaggaa agtagcgtct gattcatggt aaaaaaacct
    45661 cactaaaatt atggttacca gaatcagggc agtcttagga gtggcggcat atagccaaaa
    45721 taacgtgagc gggtccatgc ccgacagaat cgttactctc tcccatccgg actctaaccg
    45781 tcggccccgg aattacaccg gatctgctgt cctttgagtt cgcacccaaa gcgctcgcgg
    45841 gctttcaact gagttgattt accgccggtg gggaatttcg ccccgccctg agaataagcg
    45901 gattcactat aacgctaatg attagcggca gcaacgcata gcttcacata attctggttt
    45961 atgacttacc cttatcgcac tacaatggta ctcaacacct atcagtacag ggaaactaca
    46021 atgattgacc cgaaaaaaat tgagcaaatc gctcgccagg ttcacgaatc aatgcctaaa
    46081 ggaatcaggg agttcgggga agatgtggag aaaaaaatcc gccaaaccct acaagctcag
    46141 ctgacgcgcc tcgaccttgt aagccgtgaa gagttcgacg tccaaacgca agtcctgtta
    46201 cggactcgtg agaaactggc gctgcttgaa caaagaatca gcgaactgga aaatcgcagt
    46261 actgagatca aaaagcagcc agatccagag actctccccc caacgctgta aacatttgtc
    46321 tttaatgcac tgtaaattat gaattaatcc tctaatttac agtgttttct attacattat
    46381 cgcttaatcc cattcagagt atcatcacaa aaatgaatga ttgcaattga aaatataatt
    46441 gttatgtatt ctttaagttt tacttaatcg ata
//
LOCUS       DAEBXN010000024        45785 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.024, whole genome shotgun sequence.
ACCESSION   DAEBXN010000024 DAEBXN010000000
VERSION     DAEBXN010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45785
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.024"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            95..2248
                     /locus_tag="KJL99_001321"
     CDS             95..2248
                     /locus_tag="KJL99_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HBD4436107.1"
                     /translation="MYKSLFTIIILLYSASSYAKSESPPASTITFIAQIDHVELNKTA
                     LSHDMKLVLADRFYFKTSNPCETNSISARPESMDITDKAYADKLKKLETPFLDDRYFF
                     MTINQCDGNGTPMLTDIKVCTTELCGAEYTKKMSTVWLDETLKPTAKRLAIYFIQSPL
                     PYDEEKKQWKVTGWYISSTDNQSSTKQIAFEGYTDTDNLSDSKFISSFKSWFESGKIK
                     NIANYNPKGERDGKYESYYNSNGNVYESLSFSNGLINGEFITYYENGAIESKRHFNND
                     KIKDGECPHFYEDGKIKQQHSYLNSKLDGPAYEYYPDGKLMQENFYQQSVLIGNDTSY
                     YQSGKISSIHNRNSRGQYDGANERYSEEGKLLSKSVYKDGKQISVQTWYENGQKEEEK
                     HFDEQGQLNGLVKQWYKNGNLAKSQNYKHDILDGNSEEWYENGIPESLYPYKNGKTDG
                     IAKSWNKYGKLTYSIEYKNGVENGAYRNWSKNTGKLTKETLYVNGIRQGVEKEFNDRT
                     GKLLTSTQYVNNKRHGTEETYDQNGIKYITCYQNDQKLSSLDNPTQIKDNATTGDSSA
                     QFALGKYEFICANIDEGIKWLTKSAEQKNTDAIYFLATAYKGNGIPANNEKYLAYLQQ
                     AATLGSSNAQAEIGYLYLIGKELPQNLPDAGVWFKKAAAQGNFAAHFYLGRMYQNGDG
                     VERNMEKARFHLSNAAEGGIEPALKALNELEHQTK"
     gene            2301..4031
                     /locus_tag="KJL99_001322"
     CDS             2301..4031
                     /locus_tag="KJL99_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4436108.1"
                     /translation="MKAKSTLLLMILSVFYSHQVLSSEPTQPDVYSVVEKKLNSALPL
                     EENTTFRSQAEWFNLQYELLVSGYPERAYNLLSKLETESKNAKYYLDLTASRVAEEEA
                     PETALTFLSKIGLNKPATLTSFESYINSWIKSKQPEKAMQLLSLDENALNYYLPTVLV
                     AYQIVPDKAISIYNSVYGDNVVIPYQQVTMLLTVAEGYQTKGDIKNAIHYADKALNMF
                     DKAIAVDSSRESHYSDEYLKLMNIYAANGNKEKAVVLSQRLHKAITESQSNYLSTLEG
                     ILLFYRNNDMQQDYQNSLSNYIVFIDKIFAFSPSTRSELSLINLLNSLNEVELMRKRL
                     AFLMTAPEYACYNSQYCYEDKVSALKILYRHHDNELMDKYFTTLLKEISNQPLEDWDI
                     IISNTGEKFSEVGLTKYAQHLASFAEEFYIKEQKSHSESEMRGLFSSLAELYSFGNDT
                     ASANRVFHQHVPAFNTDHMIQYFINAKEWNNARELLIKEEMLGMHNLILLENICMQKN
                     AECMAHITFTLNKLTTQPAITKIDSVGNEQLYQIGKIYHKLEIKPEPEQQVLIQSLYD
                     RASGSASATQ"
     gene            complement(4091..4582)
                     /gene="yajQ"
                     /locus_tag="KJL99_001323"
     CDS             complement(4091..4582)
                     /gene="yajQ"
                     /locus_tag="KJL99_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HBD4436109.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            4750..5661
                     /gene="panE"
                     /locus_tag="KJL99_001324"
     CDS             4750..5661
                     /gene="panE"
                     /locus_tag="KJL99_001324"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBD4436110.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            5624..6214
                     /gene="yajL"
                     /locus_tag="KJL99_001325"
     CDS             5624..6214
                     /gene="yajL"
                     /locus_tag="KJL99_001325"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HBD4436111.1"
                     /translation="MSASALVCLAPGSEETEAVTTINLLVRGGIKVTTASVDSDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGTECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(6268..7716)
                     /gene="thiI"
                     /locus_tag="KJL99_001326"
     CDS             complement(6268..7716)
                     /gene="thiI"
                     /locus_tag="KJL99_001326"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HBD4436112.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQAAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            7922..8164
                     /gene="xseB"
                     /locus_tag="KJL99_001327"
     CDS             7922..8164
                     /gene="xseB"
                     /locus_tag="KJL99_001327"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HBD4436113.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            8164..9063
                     /gene="ispA"
                     /locus_tag="KJL99_001328"
     CDS             8164..9063
                     /gene="ispA"
                     /locus_tag="KJL99_001328"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HBD4436114.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQALKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            9088..10950
                     /gene="dxs"
                     /locus_tag="KJL99_001329"
     CDS             9088..10950
                     /gene="dxs"
                     /locus_tag="KJL99_001329"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HBD4436115.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLVI
                     LNFGTLMPETAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            11005..11922
                     /gene="yajO"
                     /locus_tag="KJL99_001330"
     CDS             11005..11922
                     /gene="yajO"
                     /locus_tag="KJL99_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HBD4436116.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHCWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKP"
     gene            complement(12033..12551)
                     /gene="pgpA"
                     /locus_tag="KJL99_001331"
     CDS             complement(12033..12551)
                     /gene="pgpA"
                     /locus_tag="KJL99_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HBD4436117.1"
                     /translation="MTILSRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGSMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(12529..13506)
                     /gene="thiL"
                     /locus_tag="KJL99_001332"
     CDS             complement(12529..13506)
                     /gene="thiL"
                     /locus_tag="KJL99_001332"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HBD4436118.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFVTP"
     gene            complement(13584..14003)
                     /gene="nusB"
                     /locus_tag="KJL99_001333"
     CDS             complement(13584..14003)
                     /gene="nusB"
                     /locus_tag="KJL99_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HBD4436119.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(14023..14493)
                     /gene="ribE"
                     /locus_tag="KJL99_001334"
     CDS             complement(14023..14493)
                     /gene="ribE"
                     /locus_tag="KJL99_001334"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HBD4436120.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(14582..15685)
                     /gene="ribD"
                     /locus_tag="KJL99_001335"
     CDS             complement(14582..15685)
                     /gene="ribD"
                     /locus_tag="KJL99_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HBD4436121.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGVLLQAGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(15689..16138)
                     /gene="nrdR"
                     /locus_tag="KJL99_001336"
     CDS             complement(15689..16138)
                     /gene="nrdR"
                     /locus_tag="KJL99_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HBD4436122.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDYVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            16289..16828
                     /locus_tag="KJL99_001337"
     CDS             16289..16828
                     /locus_tag="KJL99_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HBD4436123.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            17126..18010
                     /gene="tsx"
                     /locus_tag="KJL99_001338"
     CDS             17126..18010
                     /gene="tsx"
                     /locus_tag="KJL99_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HBD4436124.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(18187..18534)
                     /locus_tag="KJL99_001339"
     CDS             complement(18187..18534)
                     /locus_tag="KJL99_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HBD4436125.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(18663..19634)
                     /gene="secF"
                     /locus_tag="KJL99_001340"
     CDS             complement(18663..19634)
                     /gene="secF"
                     /locus_tag="KJL99_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HBD4436126.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALTHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(19645..21492)
                     /gene="secD"
                     /locus_tag="KJL99_001341"
     CDS             complement(19645..21492)
                     /gene="secD"
                     /locus_tag="KJL99_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HBD4436127.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(21520..21852)
                     /gene="yajC"
                     /locus_tag="KJL99_001342"
     CDS             complement(21520..21852)
                     /gene="yajC"
                     /locus_tag="KJL99_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HBD4436128.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(21875..23002)
                     /gene="tgt"
                     /locus_tag="KJL99_001343"
     CDS             complement(21875..23002)
                     /gene="tgt"
                     /locus_tag="KJL99_001343"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HBD4436129.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(23057..24127)
                     /gene="queA"
                     /locus_tag="KJL99_001344"
     CDS             complement(23057..24127)
                     /gene="queA"
                     /locus_tag="KJL99_001344"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HBD4436130.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            24220..24793
                     /gene="acpH"
                     /locus_tag="KJL99_001345"
                     /pseudo
     CDS             24220..24793
                     /gene="acpH"
                     /locus_tag="KJL99_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
     gene            complement(24798..26615)
                     /gene="malZ"
                     /locus_tag="KJL99_001346"
     CDS             complement(24798..26615)
                     /gene="malZ"
                     /locus_tag="KJL99_001346"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HBD4436131.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNLGTGGACHNSES
                     PWRDWYSFSDDGTALDWLGYASLTKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYRRMIVLRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEASEV
                     VLPASPFLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(26771..28144)
                     /gene="proY"
                     /locus_tag="KJL99_001347"
     CDS             complement(26771..28144)
                     /gene="proY"
                     /locus_tag="KJL99_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HBD4436132.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(28220..29539)
                     /gene="brnQ"
                     /locus_tag="KJL99_001348"
     CDS             complement(28220..29539)
                     /gene="brnQ"
                     /locus_tag="KJL99_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HBD4436133.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVVAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFCLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(29946..31241)
                     /gene="phoR"
                     /locus_tag="KJL99_001349"
     CDS             complement(29946..31241)
                     /gene="phoR"
                     /locus_tag="KJL99_001349"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HBD4436134.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(31299..31988)
                     /gene="phoB"
                     /locus_tag="KJL99_001350"
     CDS             complement(31299..31988)
                     /gene="phoB"
                     /locus_tag="KJL99_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HBD4436135.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIKMQGLSLNPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            32178..33380
                     /gene="sbcD"
                     /locus_tag="KJL99_001351"
     CDS             32178..33380
                     /gene="sbcD"
                     /locus_tag="KJL99_001351"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HBD4436136.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGSSKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEDLDESQQQRLQHLFTTTLR
                     TLAGEHEA"
     gene            33377..36520
                     /gene="sbcC"
                     /locus_tag="KJL99_001352"
     CDS             33377..36520
                     /gene="sbcC"
                     /locus_tag="KJL99_001352"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HBD4436137.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHVRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            36646..37827
                     /gene="araJ"
                     /locus_tag="KJL99_001353"
     CDS             36646..37827
                     /gene="araJ"
                     /locus_tag="KJL99_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HBD4436138.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMSVLTELAHNVGISIPAAGHMI
                     SYYALVVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQECWRYTFL
                     LIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAWF
                     SYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIVL
                     ALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFNL
                     GSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKPL
                     G"
     gene            complement(37970..38878)
                     /gene="mak"
                     /locus_tag="KJL99_001354"
     CDS             complement(37970..38878)
                     /gene="mak"
                     /locus_tag="KJL99_001354"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HBD4436139.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNVNSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVSKAKHGDSSGVRGAAWLWPQE"
     gene            39003..39914
                     /gene="rdgC"
                     /locus_tag="KJL99_001355"
     CDS             39003..39914
                     /gene="rdgC"
                     /locus_tag="KJL99_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HBD4436140.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWLDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(39992..40447)
                     /locus_tag="KJL99_001356"
     CDS             complement(39992..40447)
                     /locus_tag="KJL99_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4436141.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(40444..40779)
                     /locus_tag="KJL99_001357"
                     /pseudo
     CDS             complement(40444..40779)
                     /locus_tag="KJL99_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(40934..41218)
                     /gene="ppnP"
                     /locus_tag="KJL99_001358"
     CDS             complement(40934..41218)
                     /gene="ppnP"
                     /locus_tag="KJL99_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HBD4436142.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(41290..41967)
                     /locus_tag="KJL99_001359"
     CDS             complement(41290..41967)
                     /locus_tag="KJL99_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HBD4436143.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(42225..42416)
                     /gene="yaiA"
                     /locus_tag="KJL99_001360"
     CDS             complement(42225..42416)
                     /gene="yaiA"
                     /locus_tag="KJL99_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HBD4436144.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(42466..42990)
                     /gene="aroL"
                     /locus_tag="KJL99_001361"
     CDS             complement(42466..42990)
                     /gene="aroL"
                     /locus_tag="KJL99_001361"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HBD4436145.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAASTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(43173..43631)
                     /locus_tag="KJL99_001362"
     CDS             complement(43173..43631)
                     /locus_tag="KJL99_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HBD4436146.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNSRGERYTPATI
                     RECLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            43751..44560
                     /gene="proC"
                     /locus_tag="KJL99_001363"
     CDS             43751..44560
                     /gene="proC"
                     /locus_tag="KJL99_001363"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBD4436147.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(44577..45692)
                     /gene="adrA"
                     /locus_tag="KJL99_001364"
                     /gene_synonym="dgcC"
     CDS             complement(44577..45692)
                     /gene="adrA"
                     /locus_tag="KJL99_001364"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HBD4436148.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMILCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLMFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
BASE COUNT        11902 a        11944 c        11201 g        10738 t
ORIGIN      
        1 tcgcttaatt ccatataact ggacgattac attagaagta aactatactt tttatgaaat
       61 atcagggata aattattcac atggaaaata catcatgtac aaatcattat ttactattat
      121 cattctactg tatagcgcca gttcatatgc aaaaagcgaa tcccctcccg caagcactat
      181 aactttcatt gcacaaatcg atcatgttga gctaaataaa acagcattat ctcatgatat
      241 gaaactcgtt cttgccgatc gtttttattt caaaaccagt aatccttgcg aaacaaattc
      301 aatttctgct cgaccagaat caatggatat tactgacaaa gcctatgccg acaaattaaa
      361 aaaattagaa actccttttc ttgatgatcg ctatttcttt atgaccatca atcagtgtga
      421 cggtaatggc actcccatgc tgacagacat aaaagtatgt acaacagaat tatgtggtgc
      481 tgaatataca aagaaaatgt ctactgtatg gctcgatgaa actttaaaac caactgctaa
      541 acgtctggca atttatttta ttcaatctcc tttaccctat gacgaggaga aaaaacaatg
      601 gaaagtcacc ggatggtata tctcatcaac tgataatcag tcttcaacta aacagattgc
      661 atttgaagga tatacagaca cagataacct ctccgattct aaatttattt cttcatttaa
      721 atcctggttt gaaagtggaa agataaaaaa cattgctaac tataatccaa aaggagaaag
      781 agatggaaaa tacgaaagtt attacaatag caatggtaac gtctatgaat ctctaagttt
      841 ttctaatggt cttatcaacg gtgagtttat tacatattat gaaaatgggg ccattgaaag
      901 taaacgtcat tttaataacg acaaaattaa agatggtgaa tgccctcatt tttatgaaga
      961 tgggaaaatc aaacagcaac atagctatct gaatagtaaa ctcgatggtc ctgcatatga
     1021 atattatcct gatggcaaac tcatgcagga aaatttctat cagcaaagtg tgttgattgg
     1081 aaacgatacc agttactacc aatccggaaa aataagctct atacacaata gaaattcccg
     1141 tggtcagtat gatggagcta acgaacgtta tagtgaagag ggtaagttac tgtctaaatc
     1201 cgtctataaa gacggaaaac aaatttctgt tcaaacctgg tatgaaaatg ggcagaaaga
     1261 agaggagaaa cattttgatg agcaaggcca attaaatggt ctggttaagc aatggtataa
     1321 aaatggtaat cttgcaaaat cacaaaacta taaacatgac atactcgatg gtaacagtga
     1381 agagtggtac gaaaatggca tccctgaaag tttgtaccct tataagaatg gaaaaaccga
     1441 cggcattgcc aaatcatgga ataagtatgg taaattaacc tattcaatcg aatacaaaaa
     1501 tggcgtagag aatggggcat atcgtaactg gagcaaaaac acaggtaaac taaccaaaga
     1561 aaccctgtac gtcaatggta tcagacaggg agtggaaaaa gagtttaacg atcgcacagg
     1621 taaactatta acctccacgc aatatgttaa taataaaaga catggtacgg aggaaactta
     1681 tgatcaaaac ggaataaaat atattacatg ctatcaaaat gatcagaaat tatcgtcttt
     1741 agacaatcca acacaaataa aagataatgc aacaacaggc gattccagtg ctcaatttgc
     1801 attagggaaa tacgaattca tctgtgcaaa tatagatgaa gggattaaat ggttaaccaa
     1861 atctgcagag caaaaaaaca ctgatgcgat atacttttta gctacagcct acaaaggcaa
     1921 tggtatacct gcaaacaatg aaaaatacct agcatatctg caacaagctg caacactggg
     1981 cagtagtaat gcccaggcgg aaattggcta tttatatctc atcggaaaag aactaccaca
     2041 gaatcttcct gatgctggtg tctggttcaa aaaagcagct gcacaaggta attttgccgc
     2101 acatttctat ctgggaagga tgtatcagaa tggtgacggc gttgagagaa atatggaaaa
     2161 agccaggttt catctatcta atgcggcaga aggtggaata gaacctgcgc ttaaagcatt
     2221 aaatgaactt gaacatcaaa caaaataaag cgctcaggga ccacgtaagg ttcatcaaac
     2281 agaaaaggat atagtttgcg atgaaagcga aatccacttt gttattaatg attttatctg
     2341 ttttttattc tcatcaggtt ttatccagcg aacccactca gccggatgtt tattctgtcg
     2401 tcgagaaaaa actaaactct gctttacctc tggaagaaaa taccaccttc agatcgcagg
     2461 ctgaatggtt taatttgcaa tatgaattat tagtatctgg ctaccctgaa cgtgcttaca
     2521 atttactgag caaacttgag acagaatcga aaaacgctaa atattatctg gatttaactg
     2581 cctccagagt cgctgaagaa gaagcaccag agacagcctt aactttttta tcaaaaattg
     2641 ggttaaataa accggctact cttacatcct ttgagagcta tatcaatagc tggataaaaa
     2701 gcaaacaacc cgaaaaggct atgcagcttc tcagcctcga tgagaatgcc ctgaattatt
     2761 atttaccgac tgtgttagtc gcatatcaga tcgttcctga taaggcaata agtatttata
     2821 attctgtata tggagataat gttgttatac cctatcagca agtcaccatg ttacttacag
     2881 ttgcggaggg ttatcaaaca aaaggcgata taaaaaacgc cattcattat gccgataagg
     2941 cgctgaatat gtttgataaa gctattgctg tagatagcag tcgtgaatcc cattatagtg
     3001 atgagtattt gaaactcatg aatatctacg ccgccaatgg caacaaagaa aaagcggtgg
     3061 ttttatctca acgtctacac aaagccatta ccgaatctca atcgaattac ttaagtactc
     3121 tggaaggaat tttattgttt tatagaaata atgatatgca gcaagactat caaaacagcc
     3181 tttcaaatta catagtattt atcgataaaa tttttgcctt ttccccatca acaagaagtg
     3241 aattaagcct cattaattta ttaaattcat taaatgaggt ggaattgatg agaaaacgtc
     3301 tggcgtttct gatgacagca ccagaatacg cttgttataa tagtcagtat tgttatgaag
     3361 acaaagtcag cgcattaaaa atcctgtatc gtcatcatga caatgagcta atggacaaat
     3421 atttcacgac tcttcttaaa gagatcagta atcaaccatt agaagactgg gatataataa
     3481 taagtaatac tggtgaaaaa tttagtgaag tgggtctaac caaatatgct caacatctcg
     3541 catctttcgc tgaagaattt tatataaaag agcaaaaatc acattctgaa agtgaaatga
     3601 gaggactatt ttcatcactt gccgaattat atagttttgg taatgatact gccagcgcaa
     3661 accgagtgtt ccatcagcat gtccctgcct tcaatacaga tcatatgatt caatatttta
     3721 taaatgccaa agaatggaac aatgccaggg aattattgat aaaagaggaa atgttgggta
     3781 tgcataatct gatattgttg gagaatattt gtatgcaaaa aaatgcagaa tgcatggcac
     3841 acattacctt tacgcttaat aaattaacaa cacaaccagc aataacaaaa atagatagcg
     3901 tgggtaatga gcaactttat cagattggca aaatctacca taagttggaa attaaacctg
     3961 agccagagca acaggtttta attcagtcgc tttatgaccg ggcatcagga agcgcctctg
     4021 caactcaata aatgcatggt caaaagggcc accacggtgg ccctgacaat cagtagctat
     4081 atccagaaag ttaatcgcgg aagtttttga actggaacgg ctgaccgaga tcgccaccgc
     4141 gcaccatcgc catgacagcc tgcaaatcat cacgagattt gccagtcacg cggatctcat
     4201 cgccctgaat ttgcgcctgc actttcagtt tgctgtcttt gatcatcttg acgatttttt
     4261 tctgagtggc gctctcaatg ccctgtttca gtttcgcttc cacaaaccag gttttaccgc
     4321 tatgaacgat attttccggt acatccagtg aactgccttc aatgccgcgc ttcagcagct
     4381 tggcacgcag aatatccagc aactgattga cctggaagtc ggactcgctc aacactttga
     4441 tggttttgct ggcgtcgttc agctcaaatg aggcttcaac gttacggaag tcaaaacggg
     4501 actccacttc gcgactcgcg ttatcgaccg cgttacgtgc ttcctgaaga tcaacttcag
     4561 agacaatatc gaaagatggc atcttttcct ctcccttcat ttttgatgcg aagcataata
     4621 cctgcaaagt taaataacca acagtcctgc accaggacgt atcccttaat tgtgcatagt
     4681 cgcaacagca tgcacaatta agggatagcc ttattgaaca cccggagtgg ttgcgggtga
     4741 ggaggaacaa tgaaaattac cgtattggga tgcggtgcct tagggcaatt atggcttaca
     4801 gcactttgta aacagggtca tgaagttcag ggctggctgc gtgtaccgca accttattgt
     4861 agtgtgaatc tggttgagac agatggttcg atatttaacg aatcgctgac cgccaacgat
     4921 cccgattttc tcgccaccag cgatctgctc ctggtgacgc tgaaagcatg gcaggtttcc
     4981 gatgccgtca aaagcctcgc atccacactg cctgtaacta cgccaatact gttaattcac
     5041 aacggcatgg gcaccatcga agaattgcaa aacattcagc agccattact gatgggcacc
     5101 accacccatg ccgcccgccg cgacggcaat gtcattattc atgtggcaaa cggaatcacg
     5161 catatcggtc cggcacggca acaggatggc gattacagtt atctggcgga tattttgcaa
     5221 accgtgttgc ctgacgttgc ctggcataac aatattcgcg ccgagctgtg gcgcaagctg
     5281 gccgtcaact gcgtgattaa tccactgact gccatctgga attgcccgaa cggtgaatta
     5341 cgtcatcatc cgcaagaaat tatgcaaata tgcgaagaag tcgcggcagt gatcgaacgc
     5401 gaagggcatc atacttcagc ggaagatttg cgtgattacg tgatgcaggt gattgatgcc
     5461 acagcagaaa atatctcgtc gatgttgcag gatatccgcg cgctgcgcca caccgaaatc
     5521 gactatatca atggttttct cttacgccgc gcccgcgcgc atgggattgc cgtaccggaa
     5581 aacacccgcc tgtttgaaat ggtaaaaaga aaggagagtg aatatgagcg catcggcact
     5641 ggtttgcctc gcccctggta gtgaagagac tgaagccgtc accacgatca acctcttggt
     5701 tcgcggcggt atcaaagtca ccactgccag cgtcgacagc gatggcaacc tggcaattac
     5761 ctgctcgcgc ggcgtgaagc tgctggcgga tgcgccgctg gtcgaagtgg ctgatggcga
     5821 atatgacgtg atcgtgctgc ctggtggcat taaaggcacg gagtgttttc gcgatagcac
     5881 tctgctggtt gaaaccgtta aacagttcca ccgttccggg cgtatcgtcg cggctatttg
     5941 cgccgcgcca gccaccgtgc tggtgccgca cgatatcttt ccgattggca atatgaccgg
     6001 cttcccgacg ctgaaagaca aaattcccgc tgaacaatgg caggacaagc gcgtcgtctg
     6061 ggatgcacgg gtaaaattgc tgaccagcca ggggccgggt acagctatcg actttggtct
     6121 gaaaattatc gacctgctgg ttgggcgcga gaaagcccat gaagtggcat cacaactggt
     6181 gatggcggca gggatttata attattacga gtagtgtcgg atgcggcaaa cgtcgcatct
     6241 gaccagatgc gacgtaaaaa ctacaaatta cgggcgatat accttcacat tgttaaagcc
     6301 ttgctcgcgc agatagagcg cctgcagacg gctcatcacc ccgcgctcac accacagcag
     6361 ccaggttttg ttctggtcga gatcgccaaa tttggtgctc agtttataga acggcagaga
     6421 aaccacatca atcccttcga ctttcagtgg cttatcttcc tgttcatcga cagaacggat
     6481 atcgagaatc acgtcgttcg ggccgaagcc gttgacggtt tccacttcca ccacttcctg
     6541 ctcggtctgc tgggcgattt cgcggatatc aacgttattc gcttcctcaa ccactttatc
     6601 gagaatactg aagtcgaact tctcttcttc cgcttcaatc ttcgatttaa ccgctttcac
     6661 cgtcgggctt ttggagatca cgccacaata ttccggcatc gtgcgagcaa agtcttcggt
     6721 gccaatctgg cgagccaggt tgatgatgtg ctctttgtcg taagaaatca gcggacgcag
     6781 gatcagcgtg tcagagacgt tatcaatcag gcgcaggttg gtcagcgtct gactggacac
     6841 ctggccgagc gcttcgccgg tgaccagcgc ctgtacgccg taacgttcag ccactttaga
     6901 tgcggcacgc accatcatac gtttgaggat aacgcccatc tgaccgtcgt cgattttctc
     6961 gagaatttcc ccgacaaccg gttcgaaatt aatggcgaca aaacgcacgc ggtgggagct
     7021 gccaaagcgg ttccacagat aatgcgccgc ctgacgcacg ccaatttcat gcgctgcgcc
     7081 gccgaggtta aagaagcagt aatgcacgcg gcagccgcga cgcatcaaca tataactgga
     7141 aacaccggag tcgaaaccac cggaaatgag cgacagcaca tcttcctggg tgccaatcgg
     7201 gaaaccacca ataccttcgt agcgaccttt aatcagcagg aggcgatcgt cttccacttc
     7261 cagatgaaca gttacatccg ggttagtcag cttcacgcgc gcggattcaa tatgctgatt
     7321 taaaccgccg ccgacgtaac gttccacatc aatcgagcta aaatcatgtt tgccacggcg
     7381 cttcacgcgt acgcagaagg ttttgccttc cagctgatcg cgatactgaa ccaacgcttt
     7441 ctcaaaaata tcgtgcatgt cggtaaacgg cacgtcttcg acttcgagaa tatggtggat
     7501 acccggaata cgggtcaggg cgtcgcgaat agtcagacgc tggttttcat ctttcgcgcg
     7561 aacttcgatg ttatcccagt ggcggacgac agcgagcgtc tcatcatagt gctttaaaac
     7621 gttacgaatg ttcccggtaa ggatttttat aaagcgcaag cgcacagatt ggcttttgat
     7681 ggtgatttcc gggaacaatt taatgataaa cttcatggcg gcaatggttc gttggcaagc
     7741 cttaagcgac ttgtataggg aaaaatacag cagcccacac ctgcggctgc atccaggcgc
     7801 ggaagtatac cactaacatc gctttgctgc gcacatcacc ttaccattgc gcgttatttg
     7861 ctatttgccc tgagtccgtt accatgacgg ggcgaaaaat attgagagtc agacattcat
     7921 tatgccgaag aaaaatgagg cgcccgccag ctttgaaaag gcgctgagcg agctggaaca
     7981 gattgtaacc cgtctggaaa gtggcgacct gccgctggaa gaggcgctga acgagttcga
     8041 acgcggcgtg cagctggcac gtcaggggca ggccaaatta caacaagccg aacagcgcgt
     8101 acaaattctg ctgtctgaca atgaagacgc ctctctaacc ccttttacac cggacaatga
     8161 gtaatggact ttccgcagca actcgaagcc tgcgttaagc aggccaacca ggcgctgagc
     8221 cgttttatcg ccccactgcc ctttcagaac actcccgtgg tcgaaaccat gcagtatggc
     8281 gcattattag gtggtaagcg cctgcgacct ttcctggttt atgccaccgg tcatatgttt
     8341 ggcgttagca caaacacgct ggacgcaccc gctgctgccg tagagtgtat ccacgcttac
     8401 tcattaattc atgatgattt accggcgatg gatgatgacg atctgcgtcg cggtttgccg
     8461 acctgccatg tgaagtttgg cgaagcaaac gcgattctcg ctggcgacgc tttacaaacg
     8521 ctggcgttct cgattctaag cgatgccaat atgccggaag tgtcggatcg cgacagaatt
     8581 tcgatgattt ctgaactggc gagcgccagc ggtattgccg gaatgtgcgg tggtcaggca
     8641 ttagatttag acgcggaagg caaacacgta cctctggacg cgcttgagcg tattcatcgt
     8701 cataaaaccg gcgcattgat tcgcgccgcc gttcgccttg gtgcattaag cgccggagat
     8761 aaagggcgtc gtgctctgcc agtactcgac aagtacgcag agagcatcgg ccttgccttc
     8821 caggttcagg atgacatcct ggatgtggta ggagatactg caacgttggg aaaacgccag
     8881 ggtgccgacc agcaacttgg taaaagtacc taccctgcac ttctgggtct tgagcaagcc
     8941 cggaagaaag cccgggatct gatcgacgat gcccgtcagg cgctgaaaca actggctgaa
     9001 cagtcactcg atacctcggc actggaagcg ctagcggact acatcatcca gcgtaataaa
     9061 taaacaataa gtattaatag gcccctgatg agttttgata ttgccaaata cccgaccctg
     9121 gcactggtcg actccaccca ggagttacga ctgttgccga aagagagttt accgaaactc
     9181 tgcgacgaac tgcgccgcta tttactcgac agcgtgagcc gttccagcgg gcacttcgcc
     9241 tccgggctgg gcacggtcga actgaccgtg gcgctgcact atgtctacaa caccccattt
     9301 gaccaattga tttgggatgt gggacatcag gcttatccgc ataaaatttt gaccggacgc
     9361 cgcgacaaaa tcggcaccat ccgtcagaaa ggcggtctgc acccgttccc gtggcgcggc
     9421 gaaagcgaat atgacgtatt aagcgtcggg cattcatcaa cctccatcag tgccggaatt
     9481 ggtattgcgg ttgctgccga gaaagaaggc aaaaatcgcc gcaccgtctg tgtgattggc
     9541 gacggcgcga ttaccgctgg catggcgttt gaagcgatga atcacgcggg cgatattcgt
     9601 cctgatatgc tggtggtcct caacgacaat gaaatgtcga tttccgaaaa tgttggcgcg
     9661 cttaacaacc atctggctca gctgctttcc ggtaagcttt actcttcact gcgcgaaggc
     9721 gggaaaaaag ttttctctgg cgtgccgccc attaaagagc tgctcaaacg taccgaagaa
     9781 catattaaag gcatggtggt gcctggcacg ttgtttgaag agctgggatt taactacatc
     9841 ggcccggtgg acggtcacga tgtgctgggg cttatcacca cgctaaagaa catgcgcgac
     9901 ctgaaaggcc cgcagttcct gcatatcatg accaaaaaag gtcgtggtta tgaaccggca
     9961 gaaaaagacc cgatcacttt ccacgccgtg cctaaatttg atccctccag cggttgtttg
    10021 ccgaaaagta gcggcggttt gccgagctat tcaaaaatct ttggcgactg gttgtgcgaa
    10081 acggcagcga aagacaacaa gctgatggcg attactccgg cgatgcgtga aggttccggc
    10141 atggtcgagt tttcacgtaa attcccggat cgttacttcg acgtggctat tgccgagcaa
    10201 cacgcagtga cctttgccgc cggtctggcg attggtggat acaaacccat tgtcgcgatc
    10261 tactccacct tcctgcaacg cgcctatgat caggtgctgc atgacgtggc aattcaaaag
    10321 ctaccggtcc tgttcgccat tgaccgcgcg ggcattgttg gtgctgacgg tcaaactcac
    10381 cagggcgctt ttgatctctc ttacctgcgc tgcataccag aaatggtcat tatgaccccg
    10441 agcgatgaaa acgaatgtcg ccagatgctc tataccggct atcactataa cgatggcccg
    10501 tccgcggtgc gctacccgcg cggcaacgcg gttggcgtgg aactgacgcc gctggaaaaa
    10561 ctaccaattg gcaaaggcat tgtgaagcgt cgtggcgaga aactggtgat ccttaacttt
    10621 ggtacgctga tgccagaaac ggccaaagtc gccgaatcgc tgaacgctac gctggtcgat
    10681 atgcgttttg tgaaaccact tgatgaagcg ttaattctgg aaatggccgc cagccatgaa
    10741 gcgctggtca ccgtagaaga aaacgccatt atgggcggcg caggtagcgg agtgaacgaa
    10801 gtgctgatgg cccatcgtaa accagtaccc gtgctgaaca ttggcctgcc ggacttcttt
    10861 attccgcaag gaactcagga agaaatgcgc gccgaactcg gcctcgatgc cgccggtatg
    10921 gaagccaaaa tcaaggcctg gctggcataa tccctactcc actcctgcta tgcttaagaa
    10981 attattcatg gcaggagtgg cagcatgcaa tacaacccct taggaaaaac cgaccttcgc
    11041 gtttcccgac tttgcctcgg ctgtatgacc tttggcgagc cagatcgcgg taatcacgca
    11101 tggacactgc cggaagagag cagccgtccc ataattaaac gcgcgctgga aggtggcata
    11161 aatttctttg ataccgccaa tagctattcc gatggtagca gcgaagagat cgttggtcgc
    11221 gcactgcggg atttcgcccg tcgtgaagac gtagtcgttg ccaccaaagt gttccatcgc
    11281 gttggtgatt taccggaagg attatcccgt gcacaaattt tgcgctctat caacgacagc
    11341 ctgcgacgtc tcggcatgga ttatgtcgat atcctgcaaa ttcatcgctg ggattacaac
    11401 acgccgatcg aagagacgct ggaagccctg aacgacgtgg taaaagccgg gaaagcgcgt
    11461 tatatcggcg cgtcatccat gcacgcttcg cagtttgctc aggcgctgga actacaaaaa
    11521 cagcactgct gggcgcagtt tgtcagtatg caggatcact ataatctgat ttatcgcgaa
    11581 gaagagcgcg agatgctgcc tctgtgttat caggagggcg tggcggtgat tccgtggagc
    11641 ccgctggcgc gggggcgact gacgcgtcca tggggagaaa ctaccgcacg actggtgtct
    11701 gatgaggtgg ggaaaaatct ctataaagaa agcgatgaaa atgacgcgca gatcgcagag
    11761 cggttaacgg gcgtcagtga agaactcggt gcaacacgag cacaagttgc gctggcctgg
    11821 ttgttaagta aaccgggcat tgccgcaccg attatcggaa cttcgcggga agaacagctt
    11881 gatgagctgc tgaacgcggt ggatatcact ttgaagccat aacagattgc cgagctggaa
    11941 acgccgtata aaccgcatgc ggtcgtagga tttaaataag agtccaggcc tgatgagacg
    12001 tgacaagcgt cacatcaggc atcggtgcac aactacgaca gaatacccag cggccagtga
    12061 tgaccgataa agtacaggat gcctgcggaa atcaccccgg cgacaatatc gtcgatcatg
    12121 atccccatgc cgccatgtac attgcgatca aaccagcgga tcggccacgg cttccacata
    12181 tccagaatac ggaaaatcac aaacccggcg gcaacccact gccagtcatt ggtcggcagc
    12241 gccatgagcg tgatccacat accaataaat tcgtcccaga caatgctgcc gtgatcgtgc
    12301 acgcccatat ctttcgccgt ttgatgacaa agatagacgc cgatacagat ccccaacatc
    12361 accaccagcg agtagagctg ccagggtaaa aaggtcatca gataccagaa agggatcgcc
    12421 gccagcgatc ccatcgagcc aggaacgatt gggcttaatc cacttccgaa tccgacagca
    12481 agcagatgcc acggattact catcttcagg cgacttttcg cgacatcttt atggcgtgac
    12541 aaaatggtca tatcctttcc agtcaaacgt gacaggttcg ccgtcacgaa taaaacaaag
    12601 cccttcgata tcggcggtca tttgcccgat acaggtaaac ggtacgccaa ggtgtccgag
    12661 agcaacatcc agcgcgccac ggttcagttc cggcaccgtg aaacacaact cgtaatcttc
    12721 accgccagag agcgcccagc gcagcgcctg ttccggttca acatggcgag aaagcgcatc
    12781 agaaaacggc agcaatgcca ggtcaatacg tgcgccgcag tcgctggctt tcacgatatg
    12841 cccgagatcg gaaatcagac cgtcagagag atcgatagct gaatttgcca gatcgcgcag
    12901 tgcctgcccc tgtaaaatac gcggcgatgg acggagatga cgtttgatca agtagtcagc
    12961 atctttagca tcggcaacct gcaaacggtt ttgcaaaatc gccagcccgg cggcgctatc
    13021 gcccggtgta ccggtcacat agatccagtc acccggtttc gccccagagc gcgttaaggc
    13081 tcgtcccatc ggaacaaagc cgtggatacc caacgtcatt gataatggcc cacgcgtggt
    13141 atcgccgcca atgagttgca tatcgtaata attgagaaga tcaaacaaac tgtcgctgaa
    13201 ggactcaagc cacgcttcgt ctacgtccgg taaggttaat gccagcgtca gccaggccgg
    13261 atcggccccc atcgctgcca gatcgcttag gttcaccgcc agtgctttat aagccagatc
    13321 agcaggatcg atatcaggga ggaaatggtt gcccgccacc agcgtatcag tgctgatcgc
    13381 cagggtctgt ttctcgggga tattgagaag agcgcaatcg tcgccgatgc ccagttcgac
    13441 atcaagacga gaacttctta cacggtcaaa ataacgggca atcagggaga actcgccaca
    13501 tgccatacgt tatgcctcag caggtaaaaa agaaaaggcc ggcgatcatg gaacagtctt
    13561 ccgtgaatct accggcctgg atattacttt ttgttagggc gaatcacagg tgctgcttta
    13621 tcgagtacgc cgttgacgaa cttatggctg tcttctgcgc cgaacgattt cgccagttcg
    13681 atcgcttcgt taatggccac tttgtatggc acatcactac gtttagacag ttcgtacagc
    13741 gcaatgcgca gtactgcttt ttctacctgg cccagttctt ccagcaggcg ggacaggtag
    13801 ggcttcatca gtccgtcgag gtatgcggtg ttagtcgcca ccccggccag cagctcacgg
    13861 aagtacagga cgtcaacgtc ttttacatcc tgttcagcca ggaactggta ttcaacatca
    13921 gcgatgtcgt tctgggacaa ctgccaggag tagagcgcct ggacggcaca ctcacgagcg
    13981 cggcgacgag cagcaggttt cacggatttc cccttactaa tttcaggcct tgatggcttt
    14041 caatacatta atcatttcaa gcgcggtcag tgcagcttct gcacctttgt tgccagcttt
    14101 ggtgccagca cgttcgatcg cttgttcaat gctttcagtg gtcagaaccc caaaagcaac
    14161 cggaatttcg ctgtcctggg caacatgcgc caggccgttg cttgcaccac cagcgacata
    14221 ttcaaagtgg gcagtgccac cacgaataac cgtacccagc gcaatcaccg cgtcgtattt
    14281 accggtttta gccagtgcac ccgccgccag cggcagctca taggcaccag gcacccaaac
    14341 aacggtaatg ttttcatctt ttacctgacc gatacgtttc agtgcgtcaa ttgcaccttc
    14401 cagcaggctg tcattgataa agttgttgaa acgcgcgatg gtgatggcga cgcgagcgtc
    14461 cggggtagca acgttagctt caataatgtt cattttcttc cttcgggttc gagtatggcc
    14521 ccgcaggggg gcggatttta gcataatatt tcgtgcgctg cttccctttc gagccgggag
    14581 atcatgcacc cactaaatgc aggcaaacat ccgggcctac atgacgtatc tctttgaatt
    14641 taaattgggg ggcgtcggct aatttctcaa gccctggcag cgagcataat ccacgggcgt
    14701 cgctgcctaa tagtttaggt gcgatataga caatcagctc atcgactaaa cccgcctgta
    14761 gcaatacgcc agcgagcgtt ggccccgctt ccacccagat gctgttaatc tgctgtttac
    14821 ccagttgcat catcagtaca accagatcca gatgaccttt atgctctgga atcagcaagg
    14881 tacgcaccgt ttccggccac tcacgagaat cttcctgcgt acgcgcgaac caggtttcgc
    14941 cgggctgctg cacaatgcga tgttccggcg tcacgcgatt ttggctatca atcacaatac
    15001 gtactggctg gcggagattt tgttgcgggt agagcgcctg agtttgttca tccagttcag
    15061 accaacgcac tgttaaggct ggatcatccg ccagcaccgt ggcgctgctg gttaaaatgg
    15121 catgactttg tgcgcgctgt cgttgtacgt cgcgccgcgc ctgaggcgaa gtgatccact
    15181 ggctttcgcc gctcgccatc gccgtgcggc catcaagcga tgcgccaagt tttaactgaa
    15241 tataaggaaa gccggtgcgc atccgcttga gaaagccttt attcaattgc tcggcttcac
    15301 tcatcatcag cccgtggctg acgtcaatgc cagcctgttg cagacggtaa agtccacgcc
    15361 cagcgacctg cgggttagga tcttgcatcg ctgcgaccac gcgcgctacg ccagcggcga
    15421 tgagtgcgtc acagcacggt ggcgtacggc catgatggct acagggttcg agtgtcacat
    15481 acgccgttgc gcctttggct ttttcacccg ccatacgcaa cgcgtgtact tcggcatgtg
    15541 gttcacccgc tcgttggtgg taaccttcac cgacaatttc gccatctttg acaatgacgc
    15601 acccgacatt cgggttgggg tgggtggtaa aacgtcctcg ttgcgccagc tttagcgccc
    15661 gcgccatgta atactcgtcc tgcacggctt agtcctccag gcgcgcgatc tcttcgccaa
    15721 attctttgat atcttcgaaa ctgcggtaga cagaggcaaa acggatatag gcgactttat
    15781 cgagcttttt caattgctcc atcaccagat tgccaatcat cttgctcggt acttcgcgct
    15841 caccggtggc gcgcagctgc gatttaatat gattgattgc catttcaacg taatcggaac
    15901 tcaccggacg tttttccagc gcccgcagca ttccgctacg caatttctct tcattaaacg
    15961 gttcacgcac gtcgttgctt tttacaacac gcggcataac cagctccgcc acttcaaagg
    16021 tggtgaaacg ttcattacac accagacact gccgacggcg gcgtacggat gaaccctcgc
    16081 ccacgagacg agagtcaatt accttagtgt ccacggcgaa acagaatggg caatgcatac
    16141 ggcgtcctga ccaggtggtt aacggaaatc tattttaccc tgaactgacg atacaacaaa
    16201 ggcgcaacgc ttttgagata aaggatcgat gctttcgctg cttttaccct ctaccattag
    16261 gctaattgcg acgagaaagg gacgacctat gaatactaac gtttttcgac tgctcctgct
    16321 gggaagcctg ttcagcctta gcgcctgtgt gcagcaaagt gaagtgcgac agatgaaaca
    16381 cagcgtcagc acgctgaacc aggagatgac gcaactcaat caagaaacag tcaaaatcac
    16441 tcagcaaaac aggctgaatg caaaatccag cagtggggtt taccttctgc ctggagcgaa
    16501 aacaccggca agactggaaa gccagatcgg tactttacgt atgtcgctgg tgaatattac
    16561 gcctgataca gatggcacca cccttacact acgtattcag ggtgagtcga atgatccctt
    16621 gcccgcattc agcggaactg ttgaatatgg gcaaattcag ggaacaatag acaactttca
    16681 ggaaatcaat gtgcagaatc aattgattaa tgcccctgcc agcgtcctcg cccccagcga
    16741 tgttgatatt ccgttacaat taaaggggat ttctgtggat cagttaggct ttgtacgtat
    16801 ccacgacatt caacctgtta tgcagtaaac gtatttcggg acgattttgt gcgtcccgca
    16861 acatcttccc cgtcattttg ttactctgct tacatcacct ggattgatag taaaagtttg
    16921 caacaagggc gaaagtcagt acaatccccg cccgaatgtg tgtaaacgtg aacgcaatcg
    16981 attacgtaaa tgatagaact gtgaaacgaa acatattttt gtgagcaatg atttttataa
    17041 taggctcctc tgtatacgaa atatttagaa acgcaatttg cgcctttttc actcccgcaa
    17101 gggattttca aacagtggca tacatatgaa aaaaacatta ctggcagccg gtgcggtact
    17161 ggcgctctct tcgtctttta ctgtcaacgc agctgaaaac gacaaaccgc agtatctttc
    17221 cgactggtgg caccagagcg ttaacgttgt cggaagctat cacacccgtt tcggaccgca
    17281 gatccgcaac gatacctacc ttgagtacga agcattcgct aaaaaagact ggttcgactt
    17341 ctatggttat gcggatgcgc cggtattctt cggcggtaac tccgatgcta aaggtatctg
    17401 gaaccacggt tctccgctgt ttatggaaat cgaaccacgt ttctccatcg acaagctgac
    17461 caatactgac cttagcttcg gtccgttcaa agagtggtac ttcgcgaaca actacattta
    17521 cgacatgggt cgtaataaag atggtcgcca gagcacctgg tacatgggtc tgggtaccga
    17581 tatcgacact ggcctgccga tgagcctgtc catgaacgtc tatgcgaaat accagtggca
    17641 gaactatggc gcagcgaacg aaaacgagtg ggacggttac cgtttcaaaa ttaaatactt
    17701 tgtgccgatt accgatctgt ggggcggtca gctgagctac atcggcttca ccaacttcga
    17761 ctggggttcc gatttagggg atgacagcgg taacgcaatc aacggtatta agacccgtac
    17821 taataactct atcgcttcca gccatattct ggctctgaac tacgatcact ggcactactc
    17881 tgtcgtagct cgttactggc acgacggtgg tcagtggaac gacgatgcag aactgaactt
    17941 cggcaacggc aacttcaacg ttcgctctac cggctggggt ggttacctgg tagtaggtta
    18001 caacttctga ttatgaaaat gccgggattt attcccggca tttctgattg ttaacaacgt
    18061 gtgctttgtt catgccggat gcggcgtaaa cgctttgtcc ggcccacaaa agcacgtaaa
    18121 ttcaatatat tgctgaaata acgtaggcct gataagcgta gcgcatcagg caattttacg
    18181 ttttaatcac ttcttcttgt tcatcatcgc tttcaggtca gcgaatgggt tgtacttcgc
    18241 ttcaccgaca tctttctgcg catcttcccc tgcgataacg gtcgtaccat actgatccgc
    18301 ttcggtatat ttcgaatgct catgatcgtg gcaatagaga cacaacaatt cccagttact
    18361 gccatcttcc gggttattgg tatggtcgtg atcaatgtgg tgaacggtaa gttcacgcag
    18421 gttagaataa acaaactcgc gggaacagcg accgcagacc cacggataga tttttaatgc
    18481 tttttcgcga tagccgcttt ctaaccgtgc atagtttttt gggatgatag ccatgaaaag
    18541 ttacctggat gaaatgtagg gttacgcgca gtttcccaag atgcggggat tttcgcaatc
    18601 tccatacatc aaaaaaaatc ccgatcttct gaccgggatt ttcaacatca acgggaactt
    18661 gattaaggca gaattgacgg ctgatccgcc ccttcttttt ccactttctg ctgcaacatg
    18721 tgttcgcgct tcatacccag tttcagagcc aacgcagatg ctacgtagat ggaagatgca
    18781 gtgccgatgg aaacaccgat aagcatggtc agcgagaagc cttccagtac cggaccaccg
    18841 aagagataca gcatcaggat aaccatcaag gtagtaccgg atgtgatcaa ggtacggtgc
    18901 agcgtctggg tcaaggacac gttaaagatt tcgtaaggcg taccgcgacg gatcttgcgg
    18961 aagttttcac gaattcggtc cgataccacg atactgtcgt taagcgagta accgataacc
    19021 gacatcaacg atgccacaat ggtcaggtca atctcgatat ggaataacga caaaataccc
    19081 agcgtaatga taacgtcgtg cgtcagcgca ataaccaccc ctgccgccag tcgccactca
    19141 aagcggaaac ctacgtacac gaggatagac agcagcgctg ccatcaacgc catcgcaccg
    19201 gtttgcgcaa ggtcagcccc cacgctcgga ccgacgaact caatacgctt cactgctgca
    19261 ttctgattgg tggattcgtt aatcaccttc agaacttggc tgcccagcac ctgaccgccg
    19321 gtttcgcctt cagcaggcgg catacggacc atgatgtcgt ggctgctacc aaagttttgc
    19381 agcattggct cttcaaaacc ggctttttgc aatgcatcac gcattacgtc aatttcagcc
    19441 ggtttttcga gcgtaatttc gataaccgta ccaccggtga aatccagccc ccagttaaag
    19501 ccgcgcacgc ccataataac gatagcagcg attaacagca gaccagagat gccgaaagcc
    19561 cagtagtccc agcgcataaa gtcatagact ttacggccgt ggtttagttg ttcaacagta
    19621 tattcctgtg ccacatcgca ctcctcagat tgacagcttc ttgacgcgct tgccgccata
    19681 tagcaggttt acgatggcac gcgtaccgac aatcgcggta aacatcgacg tcgccacacc
    19741 gataccggta gtaatcgcga accctttaat tgccccggta cccactgcgt acaggatgat
    19801 gactttaatc agcgtggtga tgttcgcatc gaagatagaa ctgaatgcgc cacgataacc
    19861 ttcatcaatt gcctgttgaa cagtacgtcc gttgctcaac tcttctttaa tacgttcgtt
    19921 gatcagtacg ttcgcatcga ccgctaccgc aagggttaag acgatacccg cgataccagg
    19981 catactcagt gttgcgcctg gcaacagcga cataataccg acgatcaaga tcaagttggc
    20041 aatcagagca ctggtcgcaa tcagaccaaa cttcttatag aagatgatca tgaacagaat
    20101 agaaaccagc agaccggcaa ggcaagcctc cagcccctgc tcaatgttct gcatacccag
    20161 ggtcgggcca attgtacgtt cttcaacaat ctgaatcggc gcgatcaacg caccggcacg
    20221 cagcagcagt gacagctgac gggcttcgtt cgggttgttg atgccggtga tacggaagct
    20281 gttacccaga cgagactgga tgttggcgat gttaatcacc tcttcctgtt tcaccagaac
    20341 cgcacgacca tttgcatctt tcttaccgct gtctttgtac tccacaaaca gggttgccat
    20401 cggtttgccg atgttgtcct tggtgaagtt agacataatg ttgccaccag cgctatcgag
    20461 cgagatgtta acctgcggct ggttgtattc gtcctggctg gaagtggagt cggtgatatg
    20521 gtcaccagtc agaattacgc gtttgtacag cacaaccggc tgaccttcgc gggtctgttt
    20581 cacttcagag tcgcccggta cgcgaccgga tgccgcagcg gcctgatcaa cattggtgtt
    20641 taccagacgg aattccagcg ttgccgtcgc gcccagaatc tctttcgcac gcgcagtgtc
    20701 ctgaataccg ggcagttcaa caacgatacg gtcagcaccc tgacgctgaa ccaccggctc
    20761 cgccacgcca agttggttta cacggttacg caggatatta atgttctgct gcaccgcata
    20821 ttcacgcgct tcactcagac gagcatcgct cattaccgca cgcagctggt tgctgccctg
    20881 gctgctaatc accaggtccg gatggcgctt gctcagatac gcaatggctt catcacgagc
    20941 tttagcatcg cggaaagtga tgctcaggcc gtagttgttt tctttacgaa cagtggtata
    21001 cgggatgccc ttttcgcgca ggtcactgcg taggctatcg atattttgtt cctggagttt
    21061 gccaagcgcg gtgtccatat ccacttccat caggaagtga acgccgccac gcaggtcaag
    21121 gccgagcttc atcggctcag catgaatagc tgccagccag cgcggcgttg ccggggcaag
    21181 gttaagcgcc acgacgtatt tgtcacccat aacgcccatt aatgcttcac gagcgcgcaa
    21241 ctgggtgtca gtggagtcga agcgcgcaag aatagcgccc tcttccagtg ccacagactt
    21301 agcagttatt ttttcttctt gtaacgtttt ctggacctgg atcagcgttt gctcactggc
    21361 ggcgactccg cgcgcaccag tgatctgaac agccggatcc tcaccaaaca ggttgggaag
    21421 cgcatacagc agaccgatga caatcaccac gatcagcatg acgtacttcc acaaaggata
    21481 acggtttaac acggcaattc ccttagggaa aaattttaat tacagcgcct tcatggtgcc
    21541 tttcggcagg acggcagcta cgaagtcacg tttaataact acttcagtgg tgtcattcag
    21601 cgcgatagca atgtagccgt tttccgctac tttggttacg cgaccaacca ggccaccgtt
    21661 cgtcagaact tcatcacctt tggcaatgga gtccatcagc tttttgtgtt ctttggtgcg
    21721 cttctgctgt ggacgcagga tcatgaaata gaaaatcaga ccgaatacca ccagcatcaa
    21781 aatcaaagac atcgggctac cttgcgccgg tgcacccgtt gccgctaccg catcagaaat
    21841 aaaaaagctc attaaatttc cctcattatt aatattaatc aacgttcaaa ggtggtactt
    21901 ctcgcccctg acgctggtaa aaatcagtta cgaagctctc taatttaccc tcttcaatag
    21961 ccttgcgtaa acccgccatc aaacgctggt agtaacgaag gttatgaatg gtgttgagtc
    22021 gcgcgcctaa tatttcgttg caacggtcga gatgatgcaa gtaagcgcgt gaataattgc
    22081 gacaggtgta gcaatcacac tcaggatcga gtgggccagt atcgctctta tatttcgcat
    22141 tgcggatttt caccacgcca tcggtcacga acaaatgacc atttcgggcg ttgcgggttg
    22201 gcattacgca gtcaaacata tcgataccgc gacgtacgcc ttcaaccagg tcttccggtt
    22261 taccaacgcc catcaggtaa cgcggtttgt ctgccggaat ttgcggacac acatgctcca
    22321 gaatacggtg catatctgct ttcggctcac ccacagccag accgccgaca gcgtagccat
    22381 caaagccgat atctaccaga cctttaacag atatatcacg taaatcttcg taaatactgc
    22441 cctgaattat accaaataac gcatttttgt taccaagact gtcaaaacgc tcacggctac
    22501 gcttcgccca acgcagagac atctccatag agcgttttgc gtaatcccag tcagcaggat
    22561 acggcgtaca ctcatcaaag atcatgacga tatccgaacc aagatcgtac tgaatctcca
    22621 tcgacttttc cgggtcgagg aaaatcgggt cgccgttgat cgggttacgg aagtgaacgc
    22681 cctgttcggt gattttacga atatcaccaa ggctgaagac ctggaagccg ccggagtcgg
    22741 taagaatcgg tcctttccac tgcataaaat cgtgcagatc gccgtgcagt ttcatgattt
    22801 cctggcccgg gcgcagccac aggtggaagg tgttgccgag gataatttgc gcgccagtgg
    22861 cttcaacttc ttccggcgtc atccctttta cggtgccgta ggtgccaaca ggcataaaac
    22921 aaggcgtttc cactacgcca cgatcaaaga ccaggcggcc acggcgtgcg cgaccgtcgg
    22981 tggtgtccag ttcaaatttc attttttctc cactacgtca gaaaaacagt ccaacgttta
    23041 aaccagcgcc gcggaattac tccccgacgc gctcattaat tgcctgcgga ttgtacgtga
    23101 taaacatcgc atcaccgtaa ctaaaaaagc gatatttctc ttctaccgcc gctttatagg
    23161 cattcatggt gtgttgataa ccggcaaagg ccgaaaccag cataatcagc gtcgactctg
    23221 gcaagtggaa gttcgtcacc agcgcatcga ccactttgta ctggaagccc ggatagataa
    23281 agatttgggt atcgtcaaag aacggttcga taagatcgtt ttttgccgcc tgtgccgcgc
    23341 tttccagcga acgtactgaa gtggtgccaa ccgcaatcac ccggttaccg cgcgctttcg
    23401 ccgccagtac cgcgtctacc acatcctgcg gtacttcagc gtattccgag tgcatgatgt
    23461 gatcttcaat ggtgtcgacg cgcaccggct ggaaggtgcc cgcaccaacg tgcaacgtca
    23521 caaacgccat ctccacgcct ttggcgcgta atttttccag caaaggctcg tcaaaatgca
    23581 gaccagcggt cggggctgca accgcgcccg gtttttcgct ataaacggtt tgataaagtt
    23641 cgcggtcagc gtcttcgtcc ggacgatcga tatacggcgg cagcggcata tggccgatgc
    23701 tgttgaggat atccagtacc gagcgttcat cattaaattc gacttcaaac agtgcgccgt
    23761 ggcgcgcggt cattgttgcg ttgatacttt cgtcatcgcc cagcaacagt tctgcgccag
    23821 gttttggcgc tttcgaggcg cgaatatgcg caagaatgcg tttgtcgtcg agcatccgct
    23881 caaccagcac ttcaatcttg ccgccgctgg ctttacgccc aaacagacgc gccgggatca
    23941 cgcgggtatt attaaaaacc agaagatcgc cggggttgag cttatcgagt aaatcggtga
    24001 aagtaccgtg cgtcagcgcg cccgtcggcc cgtccagcga cagtaaacga cagctactgc
    24061 gttcaggcat gggatagtgg gcaatcaggg attcgggcaa ttcaaaggag aaatcggtaa
    24121 cgcgcatgac actgactcag actaaaataa gagtcgggta gtctagtgcc ggggcgctct
    24181 ccctgcaacc tttacccccc tttggataga atataatcga tgaatttttt agctcacctg
    24241 catttagccc atctcgcgga aagctcgctt tctggcaatt tactggctga tttcgtacgc
    24301 ggaaatcctg aagaaagttt tccgcccgac gtcgtggctg gcattcatat gcatcgacgt
    24361 attgactgac aatctgccgg aagtccgcga agcacaggag tggtttcgta gtgaaacgcg
    24421 ccgcgttgcg cctattacgc tggatgtcat gtgggatcac tttctttccc gccactggtc
    24481 gcagctgtcg ccggactttc cgctacagga atttgtctgt tatgcccgcg agcaagtgat
    24541 gacgattttg ccggactcac cgccacgttt tatcaatctg aacaattact tgtggtcaga
    24601 gcagtggctg gtgcgctatc gcgatatgga tttcatccag aacgtgttaa acggcatggc
    24661 aagccgccgc ccacgtctgg atgccctgcg tgactcctgg tacgatttag acgctcatta
    24721 tgacgccctc gaaacccgct tctggcagtt ttatccgcgg atgatggcgc aggcgtcaca
    24781 caaggcgtta taaaacgtta gttcatccat accgtagccg aaatggcagg caaagccaga
    24841 atcccgtcag tcagttgccc atgcccttct ttgcattgcc attgcacggc attgagaaac
    24901 ggtgacgcgg gtagcaccac ttcactggcc tcgccacggt tgattgccac cagtacacgt
    24961 tgctgattca gcacgcggac aaataccact acgttatctt ccgcatacag cacctgacag
    25021 ccgccacgac gcagcgcctg acttttctta cgcagcacaa tcattcgccg gtacagcgcg
    25081 aataacgccg tatcctgctt ttccacctgc caggggaacg gtttacggca aaacggatcg
    25141 tttttaccat ccagtcccac ttcatcaccg taataaatgc acggtacacc aggccaggtg
    25201 aacagccaga ccaccgccag cggcaggcgc gcaatatccc gaccgagcag cgttttaaat
    25261 cgcgcagtat cgtggctatc gagctgatta aacatacgta attgttgttg atgagaaagc
    25321 cctgcgcggt aattatccat ccaggccata caggtttggg cgtcaatttg ctgcggatca
    25381 taagagatgt cggtattggc aagaaatccc cacaacggga atgtgaagcc acgatagttc
    25441 atggcagcat cttccacatc ggcctgtaac cattgccgtg catcgccaaa atgttcgccg
    25501 acaatatacg cttccggctg ggtttctttc gccgcttcgg tgatcccggc aacgtgctgc
    25561 atattattgc gcgccccacc cgcctccccc agcatatgca ccacatccag ccgccagccg
    25621 tccatattcc acggcgcttt cagccagtgg cggacaatac tgtcttcccc gcgataaatt
    25681 tcattcacca gactttccga ctgataatcc agcttcgtca agctggcata gccaagccag
    25741 tcgagcgccg tgccatcatc actaaacgag taccagtcgc gccagggcga ttcagagttg
    25801 tgacaagctc cccccgtgcc aagattgtgc ctgtcaaacc aggcatggga atcgccactg
    25861 tggttaaaca cgccgtccag caccagccgc attcccagct gctgcgtatt gtgtcgcaaa
    25921 cgcagcaacg ccccatcacc gccaaactgc ggatcgacat ggcgataatc ctcggtatcg
    25981 tatttatgta cgctgggagc tttaaacacc ggattgagat acagcgctgt cacgccaagc
    26041 tttttcagat acggcagttt ttcgcttatc ccgtccagat cgccgccata gaacgttgat
    26101 ccgcccgcct gcgccgtgac cggttcatcc cagtcacgca agatgatctc ttgtccggct
    26161 gcatgatggt aatagacatg atcctgttca gcttcacgag gaagactacg cgcaaaacga
    26221 tcagggaaga tctgataaaa aatctgatcc gcagcccatt gtgggccgat atcaggtaca
    26281 tcgacggcaa actgctccag tcgtgccggc ggcattcggc tgaagccctg cggtgtaaac
    26341 caacgctggc gatcgtgcca cagcagtttg aaactgtaac gccgccgggg ttgtccgctg
    26401 gagagatcaa tcgccgcacg ccatgcggtg acgcctggct gcggctgact gcgctgctta
    26461 tgcattggta ctgacatttc ttcgttatcg tgttctgtac gcagcataat gcgctgcggt
    26521 gggtcttcgc ccgtcagcca cagtgtaatg agcagttgat ctttgctttg tttaacaaat
    26581 gggggcaccg gcaggtgcca tgcatttaac atcataattc ccctgtgatg aaaatctgat
    26641 caccttgcca tagcctaatg caggatcact catcatctga aggatatttg ggggaggagg
    26701 acgggggcgg ataactttcg gaatagccgg atcaggattt cctcctgaac cggcttacag
    26761 aacgcaagta ttattggttt tcagccagct gacgatcgtg gcggcgttta aacatccagc
    26821 caatcaacag cacaacaatc cacgcgaaac cgacatacag cgagatacgc gtatccgggt
    26881 gataaccaat caacccgata ataaagagca ggaaaatcag cccgccgatg gtcgttgcta
    26941 ccccacccgg cactttaaat ttcagcgcct taacttcttc tggcggcaaa cggcgacgga
    27001 aggcgatttg cgacagcagg atcataatcc acacccatac cgtggcgaag gttgccagcg
    27061 aagcgatcac caggaagacg ttttccggca tgatgtagtt cagatacacc gcaaacagca
    27121 gcgcggtagt catcaccagc accgtaaccc acggaatacc gcgacgcgac gttttgctga
    27181 aaattttcgg tgcgctgccc tgctctgcca taccgtggag catacggcct acgccaaata
    27241 cgtcactgtt aattgccgac agcgaagcgg tcagcacaac aaagttaaga atgctggcgg
    27301 caaaggtaat gcccatatgc tggaacgtca gtacgaacgg gctaccggca gtgccaacct
    27361 gattccacgg gtagatagac ataatgacga acagcgtacc gacgtagaac accagaatac
    27421 gcatcggcac ggagttaatc gcacgcggaa tcgatttctc aggatctttc gcttcaccgg
    27481 cggtaatccc gataatttcg atcccaccgt aagcaaacat caccatttgc aacgacatta
    27541 ccatgccaag ccagccgtta ctgaagaagc cgccgttgct ccacaggtta tgaataccgg
    27601 tcggttgccc gccgttgcca atcccccaga tgatgatgcc gaaaccggcg acaatcatga
    27661 tgatgatggt ggcgacttta aagaacgaga accagaattc cagctcaccg aagaccttca
    27721 cgctcatcag gtttacggcg caaatgatca gcaccacgct cagtacccaa atccagtgcg
    27781 gcaccgtcgg gaacaagaca cccatataga taccaaaagc ggtcacatcg gcgatggcga
    27841 caataaggat ttcaaaacag taggtccatc cggtaatgta acctgccagc gggccaaggt
    27901 tttcctgcgc ataacgcgag aaagagctgg cggccgggtt atgtaccgac atttccccca
    27961 gcgcacgcat aatgatatac gccgcgatac caccgataat ataggccaac aacacgctcg
    28021 gaccggccat tttgatggcg tctgccgaac cgtaaaacag cccggtgcca attgctgaac
    28081 ccagtgccat aaagcgtatg tggcgggtac ttagcccacg ctttagctta ttcttacttt
    28141 ccatcaattc ctaacccatc aacacaataa aaaaccacgg ggcatcgcag ccccgtggtt
    28201 aaaacaaatg ttcagtgatt tagtgagcgc tggaggtcac ctgacgacct gccgcgcgat
    28261 cccagataat ggccagaacc accatcacca ctgttggcat taaccacgcc agaccttgtt
    28321 cggccagcgg taaacgctgc gcccaggacg gtaagatatc gctgaatgca gatgctttga
    28381 tcccgtcgag aataccaaaa agcaggctga taaacatcgg cggagcaatc acgcgggacg
    28441 aattatgcca ccatgagcgt gtaaaactta atacaaccag tgcgatacac ggcggataaa
    28501 tagcggtcag caccggtacg gagatctgga tcagctggct taagccgagg ttagacacca
    28561 ccatcgagaa gccgccgagg ataaacacca gcgtacgata agagagcggt acgtactggg
    28621 caaagaattc tgcacaagca caggtcaggc caaccgccgt gaccagacag gcgatgaaga
    28681 ttaacgccgc cagcaggaag ctaccgccgc cgccaaaggt gtgctgaacg taagcatgca
    28741 gaatagcagc gccgtttgca gactgatcga ccagcgacgc gctgtctgaa cccagacaga
    28801 acagcgccag gtacagcaaa gtcagaccaa cacccgccat caggccagcc cagacggtat
    28861 aacgggtcag cagacgcgct tcggtaacgc cacgagaacg cgccgcgtta acaataacga
    28921 taccaaacac cattgcgccc agcgtatcca tggtcagata gccgttaacg aagccgttag
    28981 aaaatgcagc gttttgataa gcctcagtcg ccgtgctgat agaacccgcc ggccagacaa
    29041 tcgcgacaac agacaggatg accagcgcga taattttcag cggcgcaagg aagttgccca
    29101 cggtatccag cagcttgccc ggatagagcg aaaccagaat aacgatagcg aaatagacca
    29161 ggctgtagat aaacagcggc agcgcggaat cacccgtcag cggcgcaatc cccacttcaa
    29221 aggaaacggt agctgtacgc ggcgtagcga aaagcggccc caccgccagg taacaaaccg
    29281 ttgccagcag tacgccagcg actttaccga ttggcgtgct gaggctgtca acaccgccgc
    29341 caacttttgc cagcgccact accgttaata ccggcaggcc aacggcagta atgaggaagc
    29401 cgaatgccgc agtccagacg tgttcgcctg cctgtaaacc gaccattgga gggaaaataa
    29461 tgttacctgc gccgacgaac aacgcaaatg tcataaagcc cagagcgatg atatcgcgcg
    29521 atcttaattg atgggtcata aatcatactg cctgtggatg tggtgttgta acgttaaatt
    29581 tttgtgccaa tccctgggga tgatacagcg gtatatttaa acaattttag cgggaaatac
    29641 tcccaaccag gcgcggcgaa gaatagtttt tcgatttacc gcgttaatgc agtcagtatg
    29701 acagcacctg aagcgcaatt taaacgctta taacgtttta aagcaaggag ggatctaaaa
    29761 accagaggta tatagcgata agaaatcacc aaccagtata aaacactatt tccgtgaaaa
    29821 agacatggct aatcatgcga acaaaaaatc accaaccgtt gtaaaatcta acgcccgtat
    29881 tttgactgca aagaaaataa gccagcatcg gctggcttat ggcaataaaa gatgacaaag
    29941 gcggattaat cgctgttttt ggcaattaaa cgttccggga taacaaaact gaaacgtgtt
    30001 ccttttccta ctgtactctc aatattcagg cgactttcgt gatgattcac cgcatgtttc
    30061 acgatcgcta accctaatcc gctaccgccg gtttgccggg aacgcgcttt atcaacgcga
    30121 taaaaacgct cggtcaggcg cggaatatgc tccggtgcaa tgcccggtcc gttatcttca
    30181 acgctaaatt cggcaccgtg cggcactcgc tgccagcgta cggtgatatg cgtgccttcc
    30241 ggcgtatgat tcacggcgtt atagaccagg ttcgaaatcg cactgcgtag ctgatcttcg
    30301 ttgccagaca ccttgaggcc gttatctatc tcaaaggtaa atgtctgttt tttctgactc
    30361 agagtctgag cctcgcgctc aacaacgcgc agcatcatcg gcacatcaac cttttcattg
    30421 agcaattgcg tcggcgcggc ttcaattttc gacagcgtta gcaactgctt caccagccct
    30481 tccatccgct gggtctgctc gcgcatggtg tgcaacgctt tttcgcgtac cgcgccttcc
    30541 agcggctgct catccatcat ctccaggtaa ccctgtaaca cggtcaatgg cgtacgtaac
    30601 tcatggctca cgttggcaaa aaagttacgc cgcgcccctt ccagttgatg catttgcgtg
    30661 acatcacgcg ccaccatcag caactgtttg tgggtataag gcatgacgcg aatttccaga
    30721 tgccgcccgg tgttgagcac cagattgagc gggcgagaaa aatcacgcgt tttcagatat
    30781 tgcgtaaact ccgggtaacg cagtaggtta aggatgttct gcccgttatc ttccggccag
    30841 cgcaaaccaa gaatttgttg cgccagaccg ttacaccaga aaataccgcc ctcttccgtg
    30901 gtcagcacca ccgcgtcggg cagcgactcc gcgccgctac gaaagcgttt aatcagattg
    30961 cccagttcac ggcggcgttt tttatttcgc agctgcatct ggtgtaagcc gtatagtagc
    31021 ggttcccagc taccacgccc cggtggcggg gtcatactgc gatccaccca cagccaccat
    31081 gaaaggcgca ataaattcca gaaatgccag ataagcagtc ctgttaccga tgccagcaaa
    31141 aaccagggca ggtaaccaaa aaatgcaccc aggatgaaag ccgggaggca gcaaagtagc
    31201 agctccagca ccagcctttt ccacgacagc cgttccagca cgtaagatac tccagttaag
    31261 aaatcataag ccctgctctg cgtccgatga gcaaggcgtt aaaagcgggt tgaaaaacga
    31321 tatcctgtac cgcgcacggt ctgcaccatg cggtcatgcc cgccgggctc cagtgcttta
    31381 cgcaggcgac gaatgtggac atcgaccgtg cggtcttcca catacacgtt agttccccag
    31441 acgtggttta acagctgctc gcggctgtac acgcgctcag gatgcgtcat aaaaaagtgc
    31501 agcagtttaa attctgtcgg ccccatctcc agcggctctt cgcccgccat cactcggtga
    31561 gatgtcgggt tgagacttaa tccctgcatc ttaatcacct cttccaccgc cattggcgaa
    31621 atacggcgca ttaccgcttt gattcgcgcc accagctcct tcggcgaaaa tggcttggtg
    31681 atatagtcat ccgcgccggt ttcaaggccg cgcacgcgat cttcttcttc ccctctggcg
    31741 gtcaacatca ccactggaat atcccgggtc atcgactcgc gcttgaggtg tttgatgaac
    31801 tggataccgg agccgccagg taacatccag tcaaggagaa ttaaatccgg ccagggttca
    31861 ttcagttgat tcacagcact gtcataatct tccgcttcga ccggctgaaa gccattttgt
    31921 tcgagcacga agcagaccat ttcgcgaatt ggagcttcat cttctacgac cagaatacgt
    31981 ctcgccatga tttgccctgt tgtaataaat aggttgcgat cattaatgcg acgtcattat
    32041 gcgtcagatt tatgacagat ttatgaaaag ctcgtcgcac atatcttcag gttattgatt
    32101 tccgtggcgc agaaaaaagc aaatggcaca tctgtttggg tataatcgcg accatgcttt
    32161 ttcgccaggg aaccgttatg cgcatccttc acacctcaga ctggcatctc ggccagaact
    32221 tctacagtaa aagccgcgaa gctgaacatc aggcttttct tgactggctg ctggagacag
    32281 cacaaaccca tcaggtggat gcgattattg ttgctggtga tgttttcgat accggctcgc
    32341 cgcccagtta cgcccgcacg ttatacaacc gttttgttgt caatttacag caaactggct
    32401 gtcatctggt ggtactggca ggaaaccatg actcggtcgc cacgctgaat gaatcgcgcg
    32461 atatcatggc gttcctcaat acaaccgtgg tcgccagcgc cggacatgcg ccgcaaatct
    32521 tgcctcgtcg cgacgggacg ccaggcgcag tgctgtgccc cattccgttt ttacgcccgc
    32581 gtgacattat ttccagccag gcggggctta acggtattga aaaacagcag catttactgg
    32641 cagcgattac cgattattac caacaacact atgccgatgc ctgcaaactg cgcggcgatc
    32701 agcctctgcc catcatcgcc acgggacatt taacgaccgt gggttccagt aaaagtgacg
    32761 ccgtgcgtga catttatatt ggcacgctgg acgcgtttcc ggcacaaaac tttccaccag
    32821 ccgactacat cgcgctcggg catattcacc gcgcacagat tattggcggc atggaacatg
    32881 ttcgctattg cggttccccc attccactga gttttgatga atgcggtaag agtaaatatg
    32941 tccatctggt gacattttca aacggcaaat tagagagcgt ggaaaacctg aacgtaccgg
    33001 taactcaacc catggcggtg ctgaaaggcg atctggcgtc gattaccgca cagctggaac
    33061 agtggcgcga tgtatcgcag gagccacctg tctggctgga tatcgaaatc actactgatg
    33121 agtatctgca tgatattcag cgcaaaatcc aggcattaac cgaatcattg cctgtcgaag
    33181 tattgctggt acgtcggagt cgtgaacagc gcgagcgtgt gttagccagc caacagcgtg
    33241 aaaccctcag cgaactcagc gtcgaagagg tgttcaatcg ccgtctggca ctggaagacc
    33301 tggatgaatc gcagcagcaa cgtctgcagc atcttttcac cacgacgttg cgtaccctcg
    33361 ccggagaaca cgaagcatga aaattctcag cctgcgcctg aaaaacctga actcattaaa
    33421 aggcgaatgg aagattgatt tcacccgcga gccgttcgcc agcaacgggc tgtttgccat
    33481 taccggccca accggtgcgg ggaaaaccac cctgctggac gccatttgtc tggcgctgta
    33541 tcacgaaact ccgcgtctct ctaacgtttc acaatcgcaa aatgatctca tgacccgcga
    33601 taccgctgaa tgtctggcgg aggtggagtt tgaagtgaaa ggtgaagcgt atcgcgcgtt
    33661 ctggagccag aatcgggcgc gtaaccagcc ggacggtaat ttgcaggtgc ctcgcgtaga
    33721 gctggcgcgc tgcgccgacg gcaaaattct cgccgacaaa gtgaaagata agctggaact
    33781 gacagcgacg ttaaccgggc tggattacgg gcgcttcacc cgttcgatgc tgctttcgca
    33841 ggggcaattt gctgccttcc tgaatgccaa acccaaagaa cgcgcggaat tgctcgagga
    33901 gttaaccggc actgaaatct acgggcaaat ctcggcgatg gtttttgagc agcacaaatc
    33961 ggcccgcact gagctggaga agctgcaagc gcaggccagc ggcgtcacgt tgctcacgcc
    34021 ggaacaagtg caatcgctga cagcgagttt gcaggtactt actgacgaag aaaaacagtt
    34081 aattaccgcg cagcagcaag aacaacaatc gctaaactgg ttaacgcgtc aggacgaatt
    34141 gcagcaagaa gccagccgcc gtcaacaggc cttgcaacag gcgttagccg aagaagaaaa
    34201 agcgcaacct caactggcgg cgcttagtct ggcacaaccg gcacgaaatc ttcgtccaca
    34261 ctgggaacgc atcgcagaac acagcgcggc gctggcgcat attcgccagc agattgaaga
    34321 agtaaatact cgcttacaga gcacaatggc gcttcgcgcg agcattcgcc accacgcggc
    34381 gaagcagtca gcagaattac agcagcagca acaaagcctg aatacctggt tacaggaaca
    34441 cgaccgcttc cgtcagtgga acaacgaact ggcgggttgg cgtgcgcagt tctcccaaca
    34501 aaccagcgat cgcgagcatc tgcggcaatg gcagcaacag ttaacccatg ctgagcaaaa
    34561 acttaatgcg cttgcggcga tcacgttgac gttaaccgcc gatgaagttg ctaccgccct
    34621 ggcgcaacat gccgagcaac gcccactgcg tcagcgcctg gtcgcgctgc atgggcagat
    34681 tgttccccaa caaaaacgtc tggcgcagtt aatggtcact atccagaatg tcactctgga
    34741 acagacgcaa cgtaatgtcg cacttaacga aatgcgccag cgttataaag aaaagacgca
    34801 gcaacttgcc gatgtgaaaa ccatttgcga gcaggaagcg cgcatcaaaa cgctggaagc
    34861 ccagcgtgca cagttacagg cgggtcagcc ttgtccactt tgtggttcca ccagccaccc
    34921 ggcggtcgag gcgtatcagg cgctggagcc tggtgttaat cagtctcgat tactggcgct
    34981 ggaaaacgaa gttaaaaagc tcggcgaaga aggtgcggcg ctgcgcgggc aactggatgc
    35041 attaacgaag cagcttcagc gcgatgaaaa cgaagcgcaa agcctccgac aagatgagca
    35101 agcacttact caacaatggc aagccgtcac ggccagcctc aatatcacct tgcagccaca
    35161 ggacgatatt caaccgtggc tggatgcaca agatgagcac gaacgccagc tgcggttact
    35221 cagccaacga catgaattac aagggcagat tgccgcgcat aatcagcaaa ttatccagta
    35281 tcaacagcaa attgaacaac gtcagcagca gctcttaacg gcattggcgg gttatgcact
    35341 gacattgcca caggaagatg aagaagagag ctggttggcg acacgtcagc aagaagcgca
    35401 gagctggcag caacgccaga acgaattaac cgcgctgcaa aaccgtattc agcagctgac
    35461 accgattctg gaaacgttgc cgcaaagtga tgatctcccg cacagcgaag aaactgtggc
    35521 gctggataac tggcggcagg ttcatgaaca atgtctcgca ttacacagcc agcagcagac
    35581 gttacagcaa caggatgttc tggcggcgca aagtctgcaa aaagcccaag cgcagtttga
    35641 caccgcgcta caggccagcg tctttgacga tcagcaggcg ttccttgcgg cgctaatgga
    35701 tgaacaaaca ctaacgcagc tggaacagct caagcagaat ctggaaaacc agcgccgtca
    35761 ggcgcaaacg ctggtcactc agacagcaga aacgctggca cagcatcaac aacaccgacc
    35821 tgacgggttg gctctcactg tgacggtgga gcagattcag caagagttag cgcaaactca
    35881 ccaaaagttg cgtgaaaaca ccacgagtca aggcgagatt cgccagcagc tgaagcagga
    35941 tgcagataac cgtcagcaac aacaaacctt aatgcagcaa attgctcaaa tgacgcagca
    36001 ggttgaggac tggggatatc tgaattcgct aataggttcc aaagagggcg ataaattccg
    36061 caagtttgcc caggggctga cgctggataa tttagtccat ctcgctaatc agcaacttac
    36121 ccggctgcac gtgcgctatc tgttacagcg caaagccagc gaggcgctgg aagtcgaggt
    36181 tgttgatacc tggcaggcag atgcggtacg cgatacccgt accctttccg gcggcgaaag
    36241 tttcctcgtt agtctggcgc tggcgctggc gctttcggat ctggtcagcc ataaaacacg
    36301 tattgactcg ctgttccttg atgaaggttt tggcacgctg gatagcgaaa cgctggatac
    36361 cgcccttgat gcgctggatg ccctgaacgc cagtggcaaa accatcggtg tgattagcca
    36421 cgtagaagcg atgaaagagc gtattccggt gcagatcaaa gtgaaaaaga tcaacggcct
    36481 gggctacagc aaactggaaa gtacgtttgc agtgaaataa ctattcagca ggataatgaa
    36541 tacagagggg tgaattatct cttggccttg ctggtcgtta tcctgcaagc tatcacttta
    36601 ttggctacgg tgattggtag ccgttctggt ggttgtgatg gtggtatgaa aaaagtcatt
    36661 ttatctttgg ctctgggcac gtttggtttg gggatggccg aatttggcat tatgagcgtg
    36721 ctcacggagc tggcgcataa cgtaggaatt tcgattcctg ctgccgggca tatgatctcg
    36781 tattatgcac tggtggtggt ggtcggtgcg ccaatcatcg cactcttttc cagccgctac
    36841 tcactcaaac atatcttgtt gtttctggtg gcgttgtgcg tcattggcaa cgccatgttc
    36901 acgctctctt cgtcttacct gatgctcgcc attggtcggc tggtatccgg ctttccgcat
    36961 ggcgcatttt ttggcgtcgg agcgatcgtg ttatcaaaaa ttatcaaacc cggaaaagtc
    37021 accgccgccg tggcggggat ggtttccggg atgacagtcg ccaatttgct gggcattccg
    37081 ctgggaacgt atttaagtca ggaatgctgg cgttacacct ttttattgat cgctgttttt
    37141 aatattgcgg tgatggcatc ggtctatttt tgggtgccag atattcgcga cgaggcgaaa
    37201 ggaaatctgc gcgaacaatt tcactttttg cgcagcccgg ccccgtggtt aattttcgcc
    37261 gccacgatgt ttggcaacgc aggtgtgttt gcctggttca gctacgtaaa gccatacatg
    37321 atgtttattt ccggtttttc ggaaacggcg atgaccttta ttatgatgtt agttgggcta
    37381 gggatggtgc tgggaaatat gctaagtggc aggatttcag gacgttattc accactgcgc
    37441 attgcagcag tgactgactt tatcattgta ctggcactgc tgatgctctt tttctgcggc
    37501 ggcatgaaaa caacgtcgct tatttttgct tttatttgtt gcgcgggatt atttgccctt
    37561 tcagcacctc tgcaaatatt gttactgcaa aatgccaaag gaggagagtt attaggtgcc
    37621 gcaggtgggc aaatagcgtt taacctcggt agcgccgtcg gcgcatattg cggaggtatg
    37681 atgctgacgc tggggctggc atataattac gtggcgctgc ctgccgccct gctttcgttt
    37741 gctgcgatgt catcgttgct gctgtatggt cgctataagc gccagcaagc ggcggatagt
    37801 ccggtgctgg cgaaaccact ggggtagatt actgtcacgg tgcgcgttgc ttattgccgg
    37861 atgcggcgtg aacgccttat ccgccctacg cggttctggc acattttgta ggcctgataa
    37921 gacgcggcaa gcgtcgcatc aggcatgatg cgccaattgc ctacgttttt tactcttgtg
    37981 gccataacca cgcagcgccg cgtacgccgc tggaatcacc gtgcttcgcc ttactcaccg
    38041 gcgtttcaca ttcgccgccg aagacaaatt gtttaatcaa ctgcccaacc gtttgatata
    38101 aacggtctac attgctcatc ccgcccccca ggacaatcac atccggatcg agaatattca
    38161 cgacatgtgc cagcgatttt gccagccgca gctcgtagcg acgcaatgcc agttccgcta
    38221 ccggatcgct ttcttcaacc aggcggataa tttcactgcc tttcagcgca tgtccgctca
    38281 aacgacgata atccgtcgcg aatcccgtgc ccgaaataaa ggtttcaata caaccttgtt
    38341 taccgcaata acaagggact tcctcgcgat aacgcagttc gtcttcgtcc atccacggta
    38401 gcggattgtg tccccactca cctgccgtgc cattgccgcc gatatgcgtc cgcccattga
    38461 atgccacgcc cgcgccgcat cccgtgccga taatcacggc aaataccgtc tgcgctcccg
    38521 ctgccgcgcc atctactgct tctgaaaccg ccagacagtt ggcatcattt gccagccgca
    38581 cttcccgctg caacctcgcg cttaagtctt tatcgaatgg ctgaccgttg agccaggttg
    38641 aattgacatt cttcaccaca ccggtgtagg gcgaaatcga acctggaatg cccataccta
    38701 ccgttccgcg ctgccctgtc gcctgctccg ccatatcaac caacgtggcg atcgtttcaa
    38761 tagtctgccg gtaatcatca cgcggcgtgg gcagacgatg gcggtacaac tgctcccctg
    38821 catcgcccag tgcaatcact tcagttttgg tgccgcctaa atcgatacct atacgcactg
    38881 tactctcctt atttttttca atatcaatag cgtagagacg gacaaccgga ttggcaatgc
    38941 aaggccgccg acaattcgtt atcatgcccg ctaaatttaa cgacaaggcc gtggaaatta
    39001 tcatgctgtg gttcaaaaat ttaatggttt accgtcttag ccgcgagatt tcgctgcgtg
    39061 cagaagagat ggaaaaacag ctagcctcga tggcatttac cccatgcggc agccaggaca
    39121 tggcgaagat gggctgggtt cctccgatgg gatcgcacag cgatgcgtta acgcacgttg
    39181 ccaatggtca aattgttatc tgcgcgcgca aagaagaaaa aatcctcccg tctccggtga
    39241 ttaaacaggc gctggaagcg aaaatcgcca aactggaagc ggaacaggcg cgtaagctga
    39301 agaaaaccga aaaagattcg ctgaaagacg aagtgctgca ctctctgctg ccgcgtgctt
    39361 tcagccgttt tagccagaca atgatgtggc tcgacacggt taacggtttg attatggtgg
    39421 actgcgccag tgccaaaaaa gcggaagata cgctggcatt actgcgtaaa agcctggggt
    39481 cgttaccggt tgtaccattg agcatggaaa acccgattga actgacgctg accgaatggg
    39541 ttcgctccgg tagtgcggca cagggcttcc agctgcttga tgaagccgag ctgaaatcgt
    39601 tgctggaaga tggcggcgtg atccgcgcga agaaacaaga tctgaccagc gaagagatca
    39661 ccaatcacat tgaagccgga aaagtggtga ctaaactggc gctcgactgg cagcagcgca
    39721 ttcagtttgt gatgtgcgac gatggttcgc tcaagcgtct taagttctgc gacgagctgc
    39781 gcgatcaaaa cgaagatatc gaccgtgaag atttcgccca gcgttttgat gccgatttca
    39841 tcctgatgac tggtgaactg gcagcgttaa ttcaaaacct gattgaagga ttaggtggcg
    39901 aagcacaacg ttaattgctg attttccttt aatgccggat gcgacgcctg ccgcgtctta
    39961 tccggcgtac gaagccacac caggcatata attattcgct acggcgagca ataattttta
    40021 gcgcagcaat attatgcgtt ttacgccgta actgatctaa tcgcctcatc cctattcgcc
    40081 tgtcagcagc acgcagaata ttcactaacc cccatttatc gctggtaaat tgttttgtta
    40141 acagctccgc ttttggggta tcaatatatt cacgcaataa atcagaaatt gaacaaatat
    40201 ccgtcaggta aggataccag caagtttcac cgtgaaaatt acgcactcca gcgttacctg
    40261 caataaagtc tttcgggtaa tcccagatca cgtttttatc caacgtgatc caataacgcg
    40321 gtaaatcggt acttccacca ttttggctgc gcatgggata acgggtacag tgaatttgca
    40381 ggttgatcgt tggcgtcagt aagtcataga tctcccgctg taacttgctc catggacgtt
    40441 gtgtcattgt ttttcctcaa gccggtgacg cgcttcactg cgaattttcc gcgttgcccc
    40501 ggttttcact aaatcacgta actgcgaaag atcgggttgt tcgacaaata agagtaatga
    40561 tggatcctct tttaaccacg ccgttttcac atcggcagcc agctgtggat tattaatagc
    40621 cagcgaccga tcctgaggct cagtcaatgt cgttaacaac gacgccgggg tatgtgcgtt
    40681 gcgtaatgcc gtttggcgca aatgatcatt gcgactttgc agcaatgcac gccaccattc
    40741 atcatctgat ttatctttta tcgtagccag ttccgcaaaa aatacctgga gatcttcact
    40801 gatatttata tcaaccttga tgaattatga atatatcgga aattgaaaaa atcgcagcaa
    40861 aggaaatatt attatgggtt aaagagagga tatttcagaa aaatgccctc ccgttcaagg
    40921 ggaaggcgag gaattacaga tagcggcaca gataagaggt aggttcggca acttgaagat
    40981 gaaactcact gtgaccggga acattaaaca ccgaaccggc ttcatacact tgccagtcgg
    41041 tcgcgtcagg cagtaacaca ttcagcgcgc cactgattac cgtcatctct tccggctcag
    41101 cggtgctgaa ggtgtattcg ccttcaacca taacacccac gctggcgcga ccagtgctgc
    41161 tgctggaaaa gccgatcgat ttcactttgc cggaaaagta ctcattactt tgaagcataa
    41221 actggccctg tctggtagtt gaacatctac ttcactatag gggccagagg cgctggctgt
    41281 cacgcaaaat tacaccagta attccgcagc cagccgtgca atcaatacgt tagagagcaa
    41341 gacaggaaca tcgagctgtt tttgcagtaa atcgcgatga cgttggtgaa atcctaaaca
    41401 atccagcatg atgacatcag ccccttttgc cagtaattct ttcccagcat caatgatttt
    41461 ttgttctgaa tcatgaatgg ggttacccaa tgaaaatacc ggcgatttct gcaaaatttg
    41521 ccatttttgc gcctgaacgg gcagcatctc ctcaaccgga acgataaccc ccacctgatg
    41581 atcttcaaca atagaggaaa ccagtggagg caatattcgc gacggctcaa gaaagatcgt
    41641 attacgcgca gtcatactac taatgtttgc tgtactcatt aataaaatga cgtcataacc
    41701 ctgattatcg agcacttcaa ccacaccttg cagatctcgc tccactttac gacgcgaaac
    41761 atgggccagc tggttgtcat ttaataatgt gagaatggtg tcttcgcctg cttctggcgc
    41821 gtactccgcc atcacttctt cacgacttaa cttccccagc aggctatgat gggaaatatt
    41881 atcttcgtca atgtattccg tcaggagcgg caaaacctcc tgcataggta caatgccgat
    41941 ggtcaggatc gccaacgacg cactcattgt tcatccacct tacttttctt tcgtcgttaa
    42001 ttaccgggca agtgtgaagc accactgctg acacttactt gtgctacaaa tgacaaaaag
    42061 cgtagcagca gacgtgcacg gcataatgta aagattccaa atgattccag taatggaatg
    42121 ttattgttta atattctaat tattagaaaa acataaatta tgaaaaatgt gacgcagatc
    42181 ccttgtagtg gcactcagaa tcccttccga gcagtctgat gcggtcactc tttgtcaggg
    42241 tcctcatagc gttttttcgc atccatcgct tcctgagccg tcggatgttc actgatcaac
    42301 gagtctggtt taggatgatc ggctctgagt tggtaccagg ttaccgtctg tcctggcttt
    42361 cctttttcaa tcgtcactat atatgcttca cgaggataag gcggtttcgt tggcatcgtt
    42421 cttccttatt tcacgggatg aacgttaagt ataggcgctc taaaatcaac aattgatcgt
    42481 ctgtgccagg gcgctacgaa tttcagaaat cacctggctg ggttcgtttg ttgcgtcgat
    42541 gataatatgc gcaacttcgc gatatagcgc atcgcgttct tccagcactt cctgaacttc
    42601 ttcgctcagc ggttttcccg ttaaggttgg ccgtaaatct tcttccggtg cagcttgcag
    42661 tcggttaacc aggactgata ctggcgcaca caaataaacc acgatcccgt tattttgcat
    42721 gaagtgacga ttaaattccg tcagaataat gccgccgcct gtagcgataa cggtggatgc
    42781 cgcagttacc gcttccagcg ccgccgtttc tctggcgcga aatcccgccc actcttccct
    42841 ttcgacgatc tccgcgaccg tcatattgag ctgtgattgc aaccactgat cggtatcgac
    42901 aaaccgacgg ttaagcgaat cggcaagggc cattccgacc gttgttttac cacagccccg
    42961 aggcccgatc agaaaaagag gttgtgtcat cgtgggtttt ccccaatagg tcgcaatgcg
    43021 gcgaaagccg gtgtcatgag aatagcgatc ataccatcaa actagtacaa tttcgattgt
    43081 aaagaaaaaa ttccacttaa agtaaaaatc tcaatacatc ccttactata ccaataaata
    43141 ttcaagaatg aagtgtaaat aataaattac atttagccac gactacgttg cacttccagc
    43201 caccacttct ccagctccgc ggcaaaggcc tggcggtcac gttgtgaaag gctatctggt
    43261 ccgccggtct ggatcccact ggcacgtaag gtatccataa aatcgcgcat cgtcaggcac
    43321 tcacgaatgg tcgctggcgt gtaacgttcg ccgcgcgaat taagcgccgc tgcgcctttc
    43381 tcgatggctt cagcagccaa aggtatatct gcggtgatca ccaaatcgcc cgcttcacac
    43441 tgccggacaa tttcgttatc ggcaacgtcg aaacctgccg cgacgcgcag cgtacgaata
    43501 aatcgcgatg gcggcacgcg taaactctgg tttgctacca gtaccagcgg catctgcata
    43561 cgttccgccg cgcgatacaa aatctcttta attacattgg gacacgcgtc ggcatccacc
    43621 caaattgtca taaagtcatc ctttgttagg taatcctcta ttgtgtcgcg cttttgcctt
    43681 ccggcatagt tctgtttatg cttctgccag cgattatcaa aacaatgaat ttcacggcag
    43741 gagtgaggca atggaaaaga aaatcggttt tattggctgc ggcaatatgg gaaaagccat
    43801 tctcggcggt ctgattgcca gcggtcaggt gcttccaggg caaatctggg tatacacccc
    43861 ctccccggat aaagtcgccg ccctgcatga ccagttcggc atcaacgccg cagaatcggc
    43921 gcaagaagtg gcgcaaatcg ccgacatcat ttttgctgcc gttaaacctg gcatcatgat
    43981 taaagtgctt agcgaaatca cctccagcct gaataaagac tctctggtcg tttctattgc
    44041 tgcaggtgtc acgctcgacc agcttgcccg cgcgctgggc catgaccgga aaattatccg
    44101 cgccatgccg aacactcccg cgctggttaa tgccgggatg acctccgtaa cgccaaacgc
    44161 gctggtaacc ccagaagata ccgctgatgt gctgaatatt ttccgctgct ttggcgaagc
    44221 ggaagtaatt gctgagccga tgatccaccc ggtggtcggt gtgagcggtt cttcgccagc
    44281 ctacgtattt atgtttatcg aagcgatggc cgacgccgcc gtgctgggcg ggatgccacg
    44341 cgcccaggcg tataaatttg ccgctcaggc ggtaatgggt tccgcaaaaa tggtgctgga
    44401 aacgggagaa catccggggg cactgaaaga tatggtctgc tcaccgggag gcaccaccat
    44461 tgaagcggta cgcgtactgg aagagaaagg cttccgtgcc gcagtgatcg aagcgatgac
    44521 gaagtgtatg gaaaaatcag aaaaactcag caaatcctga tgactttcgc cggacgtcag
    44581 gccgccactt cggtgcggtt acgtccggct ttctttgctt tgtaaagcgc caaatctgcc
    44641 gatttcaacc actcacgata gtgactcatt tgtgggttca gcggcgcaac ccccacacta
    44701 atccgtaaag ttacctgtgg cgtattcggc aagcgtaacg tgtttagccc ttcacgcacc
    44761 cgtaacatgg cggtaatggc gctctcagct ggcgtaccgg acatgattac tgcaaactca
    44821 tcgccgccaa accgaccaat cacatcgcta ccgcgcaggg taatttgtaa ctgtcgggta
    44881 agcgccacaa tcgcttcatc gcccacatca tgaccccagg tatcgttgat gctcttgaaa
    44941 tggtcgatat cgataatcag taacgttgca tcgcgattat gccgccgaca gttatcaaat
    45001 tcattgcgta acatagtttc ccaatgacgt cggttataca cgcccgtcat gccgtcgcga
    45061 gtactcatga cctgcaacct gcgtttatgt tccgccagtt tggttgccgt ctggtagctg
    45121 acccagccaa acatcagagg ataaacgaca ataatgggaa gggagagcca ccattccagc
    45181 ggagcactat tgaacgacac ggtaatgccc gtaagctcga gggtgacaag gcaggaaacc
    45241 accatcaaca ccagacccgc gacaaacaga cgggggccgc ctgcccccat caaattcaga
    45301 cacagaatca tcaacatagc ggtggaaggc agcacgttta cgcccattac gcctacccac
    45361 attcccgcta atactgcatc ggtttttaag ttgtaaattt cccggctaag cggatcgacg
    45421 gccctgctcg ctatctgcca ggctaaatgc ggccagacga acgcccagcc gaccaacacc
    45481 agccaccacc agcccggcgg cgggtgcgaa accagcgttg aagcaatcgg taagaacatg
    45541 ccagccaggc caaccgcacg gggtagtctg acgcgacggg cgaagcgcag cccggaccgc
    45601 tgatgttcgt tttgaggagt taaaggaggt tcctccccat gcgccgccgc ttttttgaaa
    45661 aagttttcat cattcattat ttttgggaac attcagaaac aattttccca aattatagag
    45721 acggggctaa aggcagaagt atgaaatttc gggtgagcca ctttcgtagc tcacccgtta
    45781 gcact
//
LOCUS       DAEBXN010000025        44405 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.025, whole genome shotgun sequence.
ACCESSION   DAEBXN010000025 DAEBXN010000000
VERSION     DAEBXN010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 44405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44405
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.025"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            14..1780
                     /gene="adeD"
                     /locus_tag="KJL99_001365"
     CDS             14..1780
                     /gene="adeD"
                     /locus_tag="KJL99_001365"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HBD4436149.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     AGEISGPIVIKGRYIAGVGAEYADTPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVINGQNALLDKLDAFRYLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAASNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLALSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(1826..3217)
                     /gene="uhpT"
                     /locus_tag="KJL99_001366"
     CDS             complement(1826..3217)
                     /gene="uhpT"
                     /locus_tag="KJL99_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HBD4436150.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3355..4674)
                     /gene="uhpC"
                     /locus_tag="KJL99_001367"
     CDS             complement(3355..4674)
                     /gene="uhpC"
                     /locus_tag="KJL99_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HBD4436151.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(4684..6186)
                     /gene="uhpB"
                     /locus_tag="KJL99_001368"
     CDS             complement(4684..6186)
                     /gene="uhpB"
                     /locus_tag="KJL99_001368"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HBD4436152.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHLVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(6186..6776)
                     /gene="uhpA"
                     /locus_tag="KJL99_001369"
     CDS             complement(6186..6776)
                     /gene="uhpA"
                     /locus_tag="KJL99_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HBD4436153.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(6851..7141)
                     /gene="ilvN"
                     /locus_tag="KJL99_001370"
     CDS             complement(6851..7141)
                     /gene="ilvN"
                     /locus_tag="KJL99_001370"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HBD4436154.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIAKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(7145..8833)
                     /gene="ilvB"
                     /locus_tag="KJL99_001371"
     CDS             complement(7145..8833)
                     /gene="ilvB"
                     /locus_tag="KJL99_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HBD4436155.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPSPVWIDIPKDVQTAVFEIEAQPAVAEKAVAPAFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPRVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(8939..9037)
                     /gene="ivbL"
                     /locus_tag="KJL99_001372"
     CDS             complement(8939..9037)
                     /gene="ivbL"
                     /locus_tag="KJL99_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HBD4436156.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            9602..9691
                     /gene="tisB"
                     /locus_tag="KJL99_001373"
     CDS             9602..9691
                     /gene="tisB"
                     /locus_tag="KJL99_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HBD4436157.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            9971..11155
                     /gene="emrD"
                     /locus_tag="KJL99_001374"
     CDS             9971..11155
                     /gene="emrD"
                     /locus_tag="KJL99_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HBD4436158.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(11163..11660)
                     /locus_tag="KJL99_001375"
     CDS             complement(11163..11660)
                     /locus_tag="KJL99_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBD4436159.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(11657..12019)
                     /locus_tag="KJL99_001376"
     CDS             complement(11657..12019)
                     /locus_tag="KJL99_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HBD4436160.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(12009..12356)
                     /locus_tag="KJL99_001377"
     CDS             complement(12009..12356)
                     /locus_tag="KJL99_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HBD4436161.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            12465..12878
                     /locus_tag="KJL99_001378"
     CDS             12465..12878
                     /locus_tag="KJL99_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4436162.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDMDIDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(12925..14367)
                     /locus_tag="KJL99_001379"
                     /pseudo
     CDS             complement(12925..14367)
                     /locus_tag="KJL99_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(14402..16117)
                     /locus_tag="KJL99_001380"
     CDS             complement(14402..16117)
                     /locus_tag="KJL99_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HBD4436163.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAIGKGSFM
                     QGIEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLEGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYVGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWWHRHDPAVTFTPD
                     AKDSL"
     gene            16284..17177
                     /locus_tag="KJL99_001381"
     CDS             16284..17177
                     /locus_tag="KJL99_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBD4436164.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHSNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDISVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMSADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(17174..17988)
                     /locus_tag="KJL99_001382"
                     /pseudo
     CDS             complement(17174..17988)
                     /locus_tag="KJL99_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(17988..19604)
                     /locus_tag="KJL99_001383"
     CDS             complement(17988..19604)
                     /locus_tag="KJL99_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBD4436165.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFLEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            19899..20573
                     /locus_tag="KJL99_001384"
     CDS             19899..20573
                     /locus_tag="KJL99_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBD4436166.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLMPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEY"
     gene            complement(20612..22273)
                     /locus_tag="KJL99_001385"
     CDS             complement(20612..22273)
                     /locus_tag="KJL99_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HBD4436167.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(22469..22897)
                     /gene="ibpB"
                     /locus_tag="KJL99_001386"
     CDS             complement(22469..22897)
                     /gene="ibpB"
                     /locus_tag="KJL99_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HBD4436168.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(23009..23422)
                     /gene="ibpA"
                     /locus_tag="KJL99_001387"
     CDS             complement(23009..23422)
                     /gene="ibpA"
                     /locus_tag="KJL99_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HBD4436169.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(23416..23488)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            23727..24059
                     /locus_tag="KJL99_001388"
     CDS             23727..24059
                     /locus_tag="KJL99_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HBD4436170.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(24061..25299)
                     /locus_tag="KJL99_001389"
     CDS             complement(24061..25299)
                     /locus_tag="KJL99_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HBD4436171.1"
                     /translation="MKQITFAPRNHLLTNTNTNTNTNTNTWTPDSQWLVFDVRPSGAS
                     FTGETIERVNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFH
                     HRRGVIAEGGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERGSFTYNDHVMHQLD
                     PALDLRNVGVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGW
                     VGNHALAFIGDTLSPKGEKVPELFIVELPQDEASWKSAGDAPLSGTETTLPAPPRGVV
                     QRRLTFTHHRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRKL
                     THNKTDIQSAFNWHPSGEWLGFVLDNRIVCAHAQSGEVEYLTENHANPPSADAVVFSP
                     DGQWLAWMEGGQLWITETDR"
     gene            25400..26500
                     /gene="cbrA"
                     /locus_tag="KJL99_001390"
     CDS             25400..26500
                     /gene="cbrA"
                     /locus_tag="KJL99_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HBD4436172.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRAVFLKQPEKSNAAYWRATHKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTRFTSPTSRM"
     gene            complement(26461..27798)
                     /locus_tag="KJL99_001391"
     CDS             complement(26461..27798)
                     /locus_tag="KJL99_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD4436173.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASALCFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(27873..29021)
                     /gene="dgoD"
                     /locus_tag="KJL99_001392"
                     /pseudo
     CDS             complement(27873..29021)
                     /gene="dgoD"
                     /locus_tag="KJL99_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(29018..29635)
                     /gene="dgoA"
                     /locus_tag="KJL99_001393"
     CDS             complement(29018..29635)
                     /gene="dgoA"
                     /locus_tag="KJL99_001393"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HBD4436174.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCVGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(29619..30497)
                     /gene="dgoK"
                     /locus_tag="KJL99_001394"
     CDS             complement(29619..30497)
                     /gene="dgoK"
                     /locus_tag="KJL99_001394"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HBD4436175.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVAAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(30494..31183)
                     /gene="dgoR"
                     /locus_tag="KJL99_001395"
     CDS             complement(30494..31183)
                     /gene="dgoR"
                     /locus_tag="KJL99_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HBD4436176.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            31461..32117
                     /locus_tag="KJL99_001396"
     CDS             31461..32117
                     /locus_tag="KJL99_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4436177.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(32163..32975)
                     /gene="yidA"
                     /locus_tag="KJL99_001397"
     CDS             complement(32163..32975)
                     /gene="yidA"
                     /locus_tag="KJL99_001397"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HBD4436178.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(33089..33487)
                     /locus_tag="KJL99_001398"
     CDS             complement(33089..33487)
                     /locus_tag="KJL99_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HBD4436179.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQRNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSAQANNDLLSAGMELLKGKLFR"
     gene            complement(33727..36141)
                     /gene="gyrB"
                     /locus_tag="KJL99_001399"
     CDS             complement(33727..36141)
                     /gene="gyrB"
                     /locus_tag="KJL99_001399"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HBD4436180.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(36170..37243)
                     /gene="recF"
                     /locus_tag="KJL99_001400"
     CDS             complement(36170..37243)
                     /gene="recF"
                     /locus_tag="KJL99_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HBD4436181.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMDDTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(37243..38343)
                     /gene="dnaN"
                     /locus_tag="KJL99_001401"
     CDS             complement(37243..38343)
                     /gene="dnaN"
                     /locus_tag="KJL99_001401"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HBD4436182.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNLLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(38348..39751)
                     /gene="dnaA"
                     /locus_tag="KJL99_001402"
     CDS             complement(38348..39751)
                     /gene="dnaA"
                     /locus_tag="KJL99_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HBD4436183.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAACQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            40048..40128
                     /locus_tag="KJL99_001403"
     CDS             40048..40128
                     /locus_tag="KJL99_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4436184.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            40358..40498
                     /gene="rpmH"
                     /locus_tag="KJL99_001404"
     CDS             40358..40498
                     /gene="rpmH"
                     /locus_tag="KJL99_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HBD4436185.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            40515..40874
                     /gene="rnpA"
                     /locus_tag="KJL99_001405"
     CDS             40515..40874
                     /gene="rnpA"
                     /locus_tag="KJL99_001405"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HBD4436186.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            40838..41095
                     /gene="yidD"
                     /locus_tag="KJL99_001406"
     CDS             40838..41095
                     /gene="yidD"
                     /locus_tag="KJL99_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HBD4436187.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            41098..42744
                     /gene="yidC"
                     /locus_tag="KJL99_001407"
     CDS             41098..42744
                     /gene="yidC"
                     /locus_tag="KJL99_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBD4436188.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIVAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            42850..44214
                     /gene="mnmE"
                     /locus_tag="KJL99_001408"
     CDS             42850..44214
                     /gene="mnmE"
                     /locus_tag="KJL99_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HBD4436189.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT        10774 a        12033 c        11353 g        10245 t
ORIGIN      
        1 aaatgagaaa aatatgaata attccattaa ccataaattt catcacatta gccgggctga
       61 ataccaggaa ttgttagccg tttcccgtgg cgacgctgtt gccgattata ttattgataa
      121 tgtctctatt ctcgacctga tcaatgccgg agaaatttcc ggcccaattg tgattaaagg
      181 acgttacatt gccggtgttg gcgcagaata cgctgatact ccggctttgc agcggattga
      241 tgctcgcggc gcaacggcgg tgccagggtt tattgatgct cacctgcata ttgaatccag
      301 catgatgacg ccggtcactt ttgaaaccgc taccctgccg cgcggcctga cgaccgttat
      361 ttgcgaccct catgaaatcg tcaacgtgat gggagaagcc ggattcgcct ggtttgcccg
      421 ctgtgccgaa caggcaaggc aaaaccagta cttacaggtc agctcttgcg tgcccgctct
      481 ggaaggctgc gatgttaacg gtgccagttt tacccttgaa cagatgctcg cctggcggga
      541 ccatccgcag gttaccggcc ttgcagaaat gatggactac cctggcgtaa ttaacgggca
      601 gaatgcgctg ctcgataaac tcgatgcatt tcgctacctg acgctggacg gtcactgtcc
      661 gggtttgggt ggcaaagaac ttaacgccta tattgctgcg ggtattgaaa actgccacga
      721 aagttatcag ctggaagaag gacgccggaa attacaactc ggcatgtcgt tgatgatccg
      781 cgaagggtcc gctgccagca atctcaacgc actggcaccg ttgatcaacg aatttaacag
      841 cccgcaatgc atgctctgta ccgatgaccg taacccgtgg gagatcgccc atgaagggca
      901 tatcgatgcc ttaattcgcc gcctgatcga acaacacaat gtgccgctgc atgtggcata
      961 tcgcgtcgcc agctggtcga cggcgcgcca ctttggtctg aatcacctcg gcttactggc
     1021 acccggcaag caggccgata tcgtcctgtt gagcgatgcg cgtaaggtca cggtgcagca
     1081 ggtactggtg aaaggcgaac cgattgatgc gcaaacctta caggcggaag agtcggcgag
     1141 actggcgctg tctgccccgc catatggcaa taccattgcc cgccagccag tttccgccag
     1201 cgactttgcc ctgcaattca cgcccggaaa acgctatcgg gtcattgacg tcatccataa
     1261 cgaattgatt acgcactccc gctccagcgt ctacagcgaa aatggttttg atcgcgatga
     1321 tgtgtgcttt attgccgtac ttgagcgtta cgggcaacgg ctggctccgg cctgtggttt
     1381 gctcggcggc tttggcctga atgaaggcgc gctggctgcg acagtcagcc atgacagcca
     1441 taatattgtg gtgatcggtc gcagtgccga agagatggcg ctggcggtca atcaggtgat
     1501 tcaggatggc ggcgggctgt gcgtggtacg taacggccaa gtacaaagtc atctgccgtt
     1561 acccattgcc gggctgatga gcaccgacac ggcgcagtcg ctggcggaac aaattgacgc
     1621 cttgaaagcc gccgcccgtg aatgcggtcc gttacccgat gagccgttta ttcagatggc
     1681 gtttctttct ctgccagtga tccccgcgct aaaactaacc agtcaggggc tatttgatgg
     1741 cgagaagttt gccttcacta cgctggaagt cacggaataa taaaaaaagc ccggcgtcat
     1801 gccgggcaaa agtcaccagt tacgtttatg ccactgtcaa ctgctgaatt tttttctcgc
     1861 ggcggatttt gcgttcttcc attaccgcca ctatcgccat caggcagata caaccaatcg
     1921 cggcgatatc cagcgcggcg aaggtgcctg cccagccggt aaggccgaat accggcgtcc
     1981 catcggcaat cattcccaga cctaacttgg caaagctgtc accaatcagg taagcaaagg
     2041 tgcctttaat accatcggca gcgccaatcg cttttttagg tacaaagcca acagcagcca
     2101 caccaatcaa caattgcggg ccaaagacca ggaaacccaa cgcaaagaga gaagccagat
     2161 agatatattc gttactggca tgttgataca caccgagcgt ggcgataatc agcgccagcg
     2221 cgatgcaggc caccaggcca cggcgaccgt tcgccaggtc agagagccag ccccacagca
     2281 gcgtaccgac cagcgcacca gcttcaaaca gcgtaaagcc ctgaatcgcc accgctttag
     2341 agagtttcag ttcctggaac gcgtatacgg ttgaccactg gtcgatacca atacgtacca
     2401 catagaggaa aatgttggcg aagcacagca gccagatcac tttgtttttc agcacatact
     2461 caacaaagat ctgccacttg gtcatatcgg tagattctgt ctctttgtcc tcttcgctga
     2521 tctcctcgcc gaacagttct tcagctttgc cgaggccata agattccggg gagtcgctgc
     2581 cgtaacgcag gccgataaaa ccgacaatca gcgcgataat cgacgggaag ataaacatgc
     2641 cgatgacatg gccatcgaac aggtaatttg ccccgaacag cgccacacct gctgcgcctg
     2701 caccgccaag gttgtgagaa atattccaga aaccgaggaa tgtcccgcgt ttacgacgcg
     2761 gcgtccattt ggtgatggtg gagtaactgc acgaaccgcc ggtactctgg aaaaagccgc
     2821 ttaaggcgta gaaggcaatc atcaggaaca ggctaaccga gccgctgccc atactggcac
     2881 tgaagcccag catacaaatg gcagagagga taagcatgaa cggcaggaat tgtttggtgt
     2941 ttttgccgtc ggcgtagtat gaaaccaacg ttttacccac gccataagtg atggagaaac
     3001 ccaggccgat catccccagc tgcgtcatgc tcaacccgta ggtcgaaatc atatcgttct
     3061 gcgcgatgtt aaagttcttg cgaatcaggt acatcgtcag gtagccgata aagaccacca
     3121 ggtaggattg catgaacggt ttgaaccaca ttttgcgccg cacttcgagc ggaaggtcca
     3181 gggtcggctt gcgaacttgg tttaggaaag ccagcatggg ttactcctga aatgaatacc
     3241 tgcgtgaggc atgcattgta aaaatcagca gcaaatcagg cctgagatag cgtccaagaa
     3301 aaacctggaa aatttcttag ttttgccgga tatgaagtga aaaggtgaga tgcatcacgc
     3361 ttcacgcggt gtctgggcgt tcaaaaaggg cagtaacagc agtgcggaaa tcccggcggc
     3421 gatagagata accacaaaaa atccgctcca gtgccaggta tcgagcactt tcgccagcgg
     3481 ccaaccagca agcgacgccc ccagataagc aaacaagccg acaaaccccg tcgccgcccc
     3541 tgccgcctct ttgtgggaac actctgccgc cgccatacca attaacatct gcgggccaaa
     3601 gacaaaaaaa ccaatggtga agaagcaggt tgcctgcatc acgtagctgg caaatggcat
     3661 cagccacagc gagccgactg aaagcaaaat tccggcggcg aaaatcaaat tcatcggccc
     3721 acggttgccg ttaaacaatt tgtccgagcc ccaaccggct accagcgcac cgataaatcc
     3781 gcccagttca aacatcgtca ctgccgtatt cgccgtgacc agatcgacgc ccagcgtctc
     3841 ggacatatac aaattgcccc agtcgttgat cgccgcccgg accacataga ccagcacata
     3901 gcaaaacgaa agcagccaga tatacggatt cagcaacaca tatttggtga ggatctcttt
     3961 acgcgtcaac cctgcccctt cttgttgttg agcaatttcc agcgcgtcgt gccgccattc
     4021 accgaccgcc ggtaaaccta acgcctgcgg gcgatcgcgt agccgccagc agagaaaaat
     4081 ccccacgact atcgccatac aaccagcaat catcatcccg gcacgccagc cgtaatgcag
     4141 cgcgctcgct gccatcacaa tgggaatgag tgcgccgccg acgttatgcg ccgtgttcca
     4201 taatgcccac caaccgccgc gctcggtacg tgaataccag gccgttaaca gacgcgcaca
     4261 caccggtgaa ccccagccct ggaaaaaggc gttcagcacc cagagcacgg caaacgccca
     4321 tagcgacgtc gagaagccaa acagaatgtt catgatgccc gtggcgataa gccctatccc
     4381 cataaaataa cgggcatttg agcgatcgct gacaatgccg gagacaaact tcgacacgcc
     4441 gtaagtaatg taaaacaggg tcgctaacag gccgatatcg ctacggctga gcacgccgtt
     4501 agcaaggatt tctggtaccg cggcgttaaa acttttccgc gtgaagtaaa acagcgcgta
     4561 acccagccag atggtcagca gaatatgccg acgccagtag cgataacggg catcaatttc
     4621 atgtttatca gtcattaatg gcgcatcggc aggcgctttc agaaacggca acatcatcga
     4681 accttagaca taacgttgag gtagagaaac gctgacacgc gtgccgtgca gacaggaaat
     4741 gtgtaatgtg ccgcccagcg ccgttacgcg ctcgcgcatt ccggtgaggc caaaaccttg
     4801 ttgcccggaa cccggcggca aaccgctgcc atcgtcttca atcaccagca tcaaccgttc
     4861 atcctgctgc cagccttgca gcgtaaccgc gctggcatcg gcatgtttca caatgttgtt
     4921 cagcccttcc tggcagacac gaaacagcgt cacgcgctgg ttttcgctta atgctgattc
     4981 atcgatacgc cattcgagat ggctgacaat gccgcgccct tccagctcca tttcccgcat
     5041 cagtgagcgg atggcctgct ccagggtgag atcatccagc tggcgcggac gtaaccgtcc
     5101 taacaaacgg cgcaccgcgt cgtaaacgcc cagcgacagt tgttcgatga gctgcccgct
     5161 ctgcttcaca ctggcgttat ctgccgccag ccgctgaaca atgcccgcct gagtacgaat
     5221 agcagtgatg gtctgaccga tatcgtcgtg cagctcacgc gccacgtcgc ggcgcacgct
     5281 ctcttcggtt tctaacaaac gttcagccag atgctgattg cgcgccagtt ccttttgtag
     5341 cgactggtta agttcacgca accgctggat gcctgcgccc agtaacaacc ctgtcagact
     5401 ttgcaccagc aacgagagta ataaatccac cagatgatcg cgccaggttt gactggcgat
     5461 cagcgcgatg gcgttcatca acgtcgcaat cagcgctcct tgccagccat agtgccaggc
     5521 cagcgcgata atcggcagcg ccagacagaa tggcgtaaag cgcgacagtt cgtccggcaa
     5581 tcccaactgg agccagagac tgataacaaa cagcagcaag taccagacca gatgccgccc
     5641 gcgccagttg atgggctgag aaaccagtga cgggccgagc ggcagccagg tgttattggc
     5701 gagatagtgc cagaacacca gacatatcgg ggccagcgtc agaccgccag ttaaagtcag
     5761 caacagcgca ttccacgact ctttgccgtg ccaaagccag ggcagcgact gcaacaacgc
     5821 cgccgccgtt aacgccgcac cctgtaacag caaggtgcgc cagtcacgct gatggcgata
     5881 gcgcgagatc agcgctacgg gcagtaacgt cagcaaacta ccgatcatca ataacggaaa
     5941 atgggttaaa ccgaccgcct gcgttagcca gtaaatcagc agccactccg cacccagcaa
     6001 tacgggccag tatccgcgcg ggcattgcag cattagcccc agacgcagac caaacggaaa
     6061 taacagcacc gccatatcag ggcgctcaac cagatgcagg ctgatactcc ataggcaaaa
     6121 ccatgcggca gagaagataa aaaagcaggc aataacggta attaagcggg agaacaacgt
     6181 cttcatcacc agccatcaaa catgcggcgc gccagctcta cgtcgttact gacgcccagt
     6241 ttttccatca gattggcgcg atggacgtgt accgttttcg gtgacaagcc cagttcggcg
     6301 gcaatctctt tcaccgccat tccttgcgcc agtttttccg ccacctggcg ttcacgtttg
     6361 gtcagcgggt cctgacgacc ggatgccagt ttaatggcaa tatccggcgt cagataacag
     6421 ccgcccgtgg caaccgtatg caccgcagca atgagttcat ccgggctaca gcgtttggaa
     6481 agaaagccgc gtgcccccgc gttaagcgcc tgctcaacca gcgcaggact gtcgtgaacg
     6541 gagagcataa tcgtcgccat acctttcggc agctggctta gcagctccag accggagata
     6601 tcgggcatgg agatatcgca aatacacacc tgcacaccgc gccccggcag ccccgccagc
     6661 gcctcgcgcc ccgaaccaaa ctcggcaact acctgcaaat caggttccag ccccagcagc
     6721 tgcgcaaagc cggagcggac gatgaggtga tcgtctataa gggcaacggt gatcatggtc
     6781 ttgtcctggc gggtaaaaaa aaacgcgctt accttaacga taagcgcgat gttgttcaag
     6841 ccttgagcgg ttactgaaaa aacaccgcga tcttgttaaa catcgtcgga tcggactgat
     6901 tacgctgcac tttcacgaca tcttccagct tagcgatttg gcttatcatc tgctccagac
     6961 gctggtcgtc attgaccagt agccagatat ggcttttgtc gctgtcctga atcggcagac
     7021 aaagaatgcc ttcaacgtta aaagcgcggc gggcaaaaag gccacaaacg tgggtcatta
     7081 cgcccggatg gttgcgaacg gtgagctcca gaattacgtt gttatgagtt gtgttttgca
     7141 tggcttattc ccccaccatt tcagtattcg ccgcacctgg cggcaccatc gggtaaactt
     7201 tttcttcggc atcaatgcgc acatggatca gcgccgggcc agggcgattg atgatttcct
     7261 gcaatgcagc ctgcggatcg gcttcgttat tcaaatcaca ggtttcgagg ccgaatccgg
     7321 cggcaatctg cataaagttg attttgcccg gataggtggc ggcaaaaacg ccttgctcgt
     7381 agaacagact ctgttgctga tgcaccagcc ccagcgcttc gttgttcatc agaatgattt
     7441 tgacatccag ctgattttca ctggcggtcg ccatctcctg aatattcatc atcaggctgc
     7501 cgtcgccgga gaaacacaac actttgcgat ccgggttcgc cagcgccgcg ccaatcgccg
     7561 caggcaggcc aaaacccatc gtgcccagcc caccggaggt cagccactgg cgtgggcgat
     7621 tgagcggata agcttgcgcg gtccacatct gatgctgacc cacatcggtg gtgataatcg
     7681 cattgtcatc gacacaggcg gcaacggcgt tgatcaggcc gtaatggctt aatggatcgc
     7741 acgctttcgg gattggacac gggaattcgc gctgcaaatc cgctaccaac tggtgccact
     7801 ctgcacgcgg ttgcgcttcc acccgcggga tcaattgcgc cagcacgtca tcaacatccg
     7861 cctgaatcgc cacatgcggc tgcttgattt tacccagctc tgcgcgatcg atatcgacat
     7921 gaataatttt ggcattcgga cagaactgct cggttttgcc aatcgcccgg tcatcaaaac
     7981 gcgcaccgag cacaatcaac aaatccgcct cctgcaaaat atagttggta ctgcgcacgc
     8041 cgtgcatccc cagcataccc agcgacaacg gatgcgcttt tggcagcatg cccagcgcca
     8101 ttaaagtcat ggtggtaggc agttgcgctt tctccgccag ttcacgcacc cgcgcaggcg
     8161 cattgatcac accaccaccc agataaagca ccgggcgttt ggcagcgtta atcattgtag
     8221 ctgcgtcacg aatgctttct tcgctaaagg cgggtgcaac ggctttttct gccaccgcgg
     8281 gctgagcttc aatctcaaaa accgccgttt gtacatcctt aggaatgtct atccacaccg
     8341 ggctagggcg gcctgattgc gcaatgcgga aggcatcgct catgacctgt gggagctctt
     8401 cgatatgtct gaccagatag ttgtgtttgg tgatggggat agagatgccg taggtgtcca
     8461 cttcttggaa ggcgtcggtg ccgatcatcg aggcgggaac ctgaccagtg atgcaaatca
     8521 gcgggatgga gtccagccgc gcatcagcaa tggcggtcac caggttagtc gcacccggcc
     8581 cgctacaggc catacagacc gccggtttac cgtcggtgcg cgccattccc tgagcgataa
     8641 accccgcgcc ctgttcatgg cgagccagaa tatggcggat ttgcgtgctt tggcttaagg
     8701 cgtcgtaaac aggcaggata gaaccgcccg gaatgcccgt cacaatctta atgccctgct
     8761 gttccaggaa atgaacgata aattctgcgc cggtaaagcg cttacgcgtc gatgttgtgc
     8821 ccgaacttgc catgctccag tccttttctt ctgggccgac tttccgggag gtgcttaaac
     8881 gaaaaacccc gcccggtttg cgccggcggg gttttggaat cgtgtgttgt tccagtccct
     8941 acggcgcatt gccgacgacc accaccacac gcacgacgac cactgcggcg gatggcgcag
     9001 ttggtagtag ttttgcgttg aacatggaag tagtcattgg ggaccttgtt ggttttgtgt
     9061 ttaacaatat ttatacaagc acagctttac aggggagaca atggaaaatt tttcagcaag
     9121 ggaaaattga ggggttgatc acgttttgta ctgaattgca gataacaaaa aaccccgccg
     9181 gagcgaggtt tcgtcagccg cctgcggctg gtaaccgcaa agcacactgt attatgtcaa
     9241 cactgaaagt atacgtgttc cgcgcagaac gcgcaatttc ggcacgaatt ttgacgtatt
     9301 tagtgcatag ttgagtatcg ttcacagttt gcgttttgtc caaatattac tgtttattta
     9361 tacagtaagc ttctataata tcactgtacg caatgtgtta tgcgggggcc gcatcgttac
     9421 ccggcgcact aagtcctggc tgaaacgggt ggtgccgtca gcgccttaac cccgcgtgag
     9481 cacactgtgt tatgtcaaca agcacaacgt ttctccttga gataccgcgt gcacaacagc
     9541 tggcaacagg cagcggaaag gtacgtcagc tggcagtgct cctgaaccac aggagacgcg
     9601 tatgagcctg gtggatatcg ccattcttat cctcaaactc attgttgcag cactgcaact
     9661 gcttgatgct gttctgaaat acctgaagta attcagattc aagtcgcacc aaaggggagc
     9721 gggtaaccgc tcccctttta tatttagcgt gcgggttgga gtcggatgcg atgctgacgc
     9781 atcttatccg ccctaccatc cctcccggca acatttattg ccgcttttgt ttacatattc
     9841 tgccgctaaa caattcccca ttcctggcgt atatctggct aacattcatc aatgtgatag
     9901 attcctctcc cgcatttatg ggaatgcgta gtgacttatt ctaattattt ttataaaagc
     9961 atccgtgata atgaaaaggc aaagaaacgt caatttgtta ttgatgttgg tattactcgt
    10021 ggccgtcggt cagatggcgc aaaccattta tattccagct attgccgata tggcgcgcga
    10081 tctcaacgtc cgtgaagggg cggtgcagag cgtaatgggc gcttatctgc tgacttacgg
    10141 tgtctcacag ctgttttatg gcccgatatc cgaccgtgtc ggtcgccgac cagtgatcct
    10201 cgtcggaatg tccattttta tgctggcaac gctggtcgcg gtcacgacct ccagtttgac
    10261 ggtattgatt gccgccagcg cgatgcaggg gatgggcacc ggcgttggcg gcgtaatggc
    10321 gcgtactttg ccgcgtgatt tatatgaacg gacacagttg cgccatgcta acagcctgtt
    10381 aaacatgggg attctcgtca gtccgttgct cgcaccgcta atcggcggtc tgctggatac
    10441 gatgtggaac tggcgcgcct gttatctctt tttgttggtt ctttgtgctg gtgtgacctt
    10501 cagtatggcc cgctggatgc cggaaacgcg tccggtcgat gcaccgcgca cgcgcctgct
    10561 taccagttat aaaacgcttt tcggtaacag cggttttaac tgttatttgc tgatgctgat
    10621 tggcggtctg gccgggattg ctgcctttga agcctgctcc ggcgtgctga tgggcgcggt
    10681 gttagggctg agcagtatga cggtcagtat tttgtttatt ctgccgattc cggcggcatt
    10741 ttttggggca tggtttgccg ggcgtcctaa taaacgcttc tcaacgttga tgtggcagtc
    10801 ggttatctgc tgcctgctgg ctggcttgct gatgtggatc cccgactggt ttggcgtgat
    10861 gaatgtctgg acgctgctcg ttcccgccgc gctgttcttt ttcggtgccg ggatgctgtt
    10921 tccgctggcg accagcggcg cgatggagcc gttccccttc ctggcgggca cggctggtgc
    10981 gctggtcggc ggtctgcaaa acattggttc cggcgtgctg gcgtcgctct ctgcgatgtt
    11041 gccgcaaacc ggtcagggca gcctggggtt gttgatgacc ttaatgggat tgttgatcgt
    11101 gctgtgctgg ctgccgctgg cgacgcggat gtcgcatcag gggcagcccg tttaagcgca
    11161 cgtcaccgca gcatcgtcat cagctccatg ggagaacgat gctgctttat cagatcacgc
    11221 atcacccgca tatgcggtgc ggagtaagaa taaaacgcct gatagcccgc acaaagcacg
    11281 ctgtctggcg tctcctgccg agcgtgcggg cacaggcgat gccagtcgca cgcctggcag
    11341 tcacggcgcg gttgcgggtt ttcgctgcca caccattttt gtaaggtggc gtcaaaaagc
    11401 tcaatcgaga tacgactgat atctgcccgc acccaacact caaacagggc aattaaaaat
    11461 ttcccccaca gctctgctgt caccgaagcg gccgtcagat cgccccggtc atcaaattca
    11521 agatagggaa tgagctgtac ggagcggatg ccccgctgtt tgaaattgtg ataaaaatcg
    11581 gcaggttgca gagcatcctt gtactctaca accagcttat gcctgtcctc ttcagcatca
    11641 atgacctgac ttcctgtcat gcgactctct caataaatac gataagttga tgtatatgcg
    11701 ttacagcaaa cagtattgcg agagataaca ccgcgaggga gatcaaaaat ttgtcacgga
    11761 ctacgtgaaa ttgggaaaaa tcgctattcg tgacatccat taaattgcga ttacgggtgt
    11821 agtgccagag gatcagcgcc acgatggcga ggatgccaat cgaaatccag aataacatgc
    11881 ccgcctggtg ccagttgtgt ttgatagcca acgccatcaa cgcgccgtag cccagcatgg
    11941 tacgaaacca cgccagtgat gtgcgctccg gctgcagccc tgggtcggca atgcgtctgg
    12001 ctttacggct atccggcata caacaccagt cccataacga tcaccgccac aaccatcaat
    12061 attaagctga tgattaataa gctgttggtg tagggcaggt cctctttcag acgcatcgct
    12121 ttttcattgc gtaaccagcg caggtagcca tacattgcta acccgccgga aaacagacac
    12181 aacaacaacg ccagcagttc acgaatgacg ggcgtggcga aatctggcgc aagttgatca
    12241 agcccaaccc ccgccgccag aaatcctagt gcggtacgga tccacgccag aaaagtacgc
    12301 tcattagcca gcgagaagcg gtaatcgggc gcttctccaa ggcgggaaat tttcattgtt
    12361 gctcctttgc aatttcacta acgctctatc tttagcggtt ttttttaaga atttcgtccg
    12421 cccgtttcct gatatgcttt tgccatccgc tacaaggagg tcagatgggt atcatagcgc
    12481 aaaataaaat cagttcattg ggcatgctgt tcggtgccat cgctttaatg atggggatta
    12541 tccacttttc attcggccct ttttccgcac caccgccaac gcttgaaagc atcgtggcgg
    12601 acaaaacggc tgaaattaaa agaggtcttc tcgccggaat aaaaggcgaa aaaattacca
    12661 ctgtggagaa aaaagaggac atggatatcg ataaaatcct cgatcaaagc ggtatcgcac
    12721 tggccattgc tgcgctactt tgcgcattta ttggcggtat gcgtaaggaa aatcgctggg
    12781 gcattcgcgg cgcgctggtt tttggtatcg gcatcgtgtg cagcattttg ctgatattcc
    12841 tgattttttc cttcctgacc ggcggttcgc tggtttaagc agcctgaata atgcccgccg
    12901 ggtgatcatc agcgggcatt atcgtcagaa tttctgtttt ttctcctcca ccttcactcc
    12961 ttgcgtcggt aggccggtgt catagtcgcg taccactggc gaatagccat cttgtgggcg
    13021 tgggcgaaac gcccccatcc agcgcggtcg tgcatcttta cgccacggac gcagactcca
    13081 ttggtaactg cggaacggat cgcgaatttt gtccatgtaa tccaataagg cgtcatgcat
    13141 tttgctgcga acgtctgcaa aacggatatc atcgatcagg ttatgcattt catttgggtc
    13201 attacggcga tcgtaaagtt catcactggt gaagaggttg agcaccagtt taaagtcatc
    13261 cgtcacccag caacgcaccg gaataaaacc gccaaagctg tcatgctcaa tctcgtagcg
    13321 gttaaattcc accatcacgc cgcgtggctc tttcacggca aggatatttt cccccggcag
    13381 aatctctggt ttttcaatat ctgccagcgc catcattgtc ggcagtaaat caatatgact
    13441 gactggcgta tcgacctgtc gccgctcccc ttgcggcgaa cggatgatca gcggaatacg
    13501 ggtgatgtcg tcatacatcg ccgctccttt actgatcagc ttatgtgcgc ccatcatttc
    13561 accgtgatcg gaggtataaa tgacccacgt attttcacgt tgctctggcg ttaaggcatt
    13621 gatgacacgt ccgatttggt catcaacaaa gtcattgcag gcaaaataga gcggatggtg
    13681 ataaagcccg tcatcaccga ctggcgatgg catcgcctgc gcccataagc ggtgatgttc
    13741 cggtttgttc gccaggtcat cctgtgattt ctcgccaagt tcgtagtaaa aatcagcgta
    13801 tttctctaaa tactccaccg gacaggtgaa cgggtgatgc ggctcatcat acgaaaccac
    13861 catcaggaag ggttcctcgg cgcgcacggg ctattgcaga aaatctaccg ctcgattgct
    13921 gatgcggtgc gcccaggtga aggtttcgtc aatatggttc gcctgtaaat cttcgacgct
    13981 gtttaggcca ttgcgccaca ggctgatctc tttttccgtc agttcgctaa gatagttcgc
    14041 cccatcaaac cagtaatcag catcccactc cggcggacac tcgccagtgc cgaaatagtc
    14101 atgaccgtcg agatgccatt tgccgatgta acaggtgtga tagccggcat ccttaaagta
    14161 gcgccccata gtggagatgt ttttgcctgg cgcgacgttg ttggtccacg ggccggactg
    14221 gttagcgtag ataccggtga atagtccggc ccgtgcaggc gtacaaaccg gtgaacaagt
    14281 gtaggcggaa ttaaagcgaa taccttccgc cgccagacta tcaatatttt gcgtattcag
    14341 cggtttaccg ctatagcaac cgaccatatt ggtggcctgg gtatcggtca attggggcgt
    14401 ttcataggct atccttcgcg tcgggagtaa aggttacggc tgggtcgtga cgatgccacc
    14461 agctgtcaaa aatcaggtag acaatcacta cggcagcgat gaaataactg atcatcgcta
    14521 accagcgcgt accgtagccg ccgaattcag ccagcccgac atagacgcca atcatcgcga
    14581 acaggatacc aattgacgcg atcttgacgt ttttccacgg tttcatgtcc accgcaaacg
    14641 catctttgaa ggtgaacggc gtggcgcgcg gtttgataaa accgatcacc agcatcacga
    14701 ccacgttgat gcagaacgta caggccagca catagaggaa atggaagtcg aacttcacca
    14761 gatagttgat ggtgatgtag ctgattatgc caatccccat cgccactttt gccgccagcg
    14821 ccgggatgcg cggaaagaaa aagcccataa tgatgatggt aaccagcggc acgttgtaaa
    14881 tgccgttgag ctgtttcatc cagctataca gcccctgcgg cgcgttcgcg atccacggcg
    14941 ctaccaaaac cgaaacgata gcgataaaga aaccaaattt gcgcccgacg gtgaccagct
    15001 gctgcggctc ggcattctgg ttaatgatgc gacggtaaat acccatactg aataatgtac
    15061 tggcgctatt cagaaagccg ttgaaggtac tgatcaccgc accaaataac accgcgccga
    15121 agaaaccctc cagtggcact ggcagaacgt tattgaccag cgtcgggtag gccatgtcgg
    15181 ctttcggtag atcctgatac agatgaaacg caatcaaccc tggcagcacc agtaccagcg
    15241 ggtcgagcat tttcagcacc gccgttaaca gcgccccctt ttgcccttcc gccaggcttt
    15301 ttgacgccag cgtgcgttgc acgatgccct gatttgtaca ccagtaaaag gtgttcacca
    15361 gaattaaacc ggtaaatgcc gcgccaatcg gcaagggatc ggtcgggcca ccgactgagt
    15421 ttaatttctc ggcgtgaacg gtggtaattt gctcaatgcc ctgcataaag ctgcccttgc
    15481 ctatcgcgat caggccaaat accggcacca tcaacccgcc gataaccagc ccaataccgt
    15541 tgatggagtc cgccactgcc attgcgcgca gtccgccgat caccgcatac aaaattcccg
    15601 ccagaccaag caaaattacc agtagccaga tagccgcacc gtgagaaatc tgtagcgatt
    15661 ccccgacgtg aaacaggctg ttgagcgcca acgcgccgga gtagagaaca atcggcagaa
    15721 agcagacgcc ggtggcaatg aggaagcaga agtcgatgat aatacgcgtc gttttatcat
    15781 aacgttcctc cagaaaatcg gggatggtgg caatgccgcg cttcagatag cgcggtaaaa
    15841 agattagcgc gaggaagatc agcgtcaccg ctgaagtcac ttcccagccc atcaccgaca
    15901 tgccgctttt gtaggcctgc ccggaaagac caaccagttg ttccgtggaa aggttggtta
    15961 acattaacga agcggcaata accggcgctt ttagtgaacg tccggcaaga aaatagcctt
    16021 gttgcgatcc ggtatctgtt ttgcgaacct tccaccaggt gatcaccgcc accagcagcg
    16081 taaaaccgac aaaactcaag atttgtaacg aattcatctc atagccctta tttatgttta
    16141 tgtcccggac gcatgaatat agggagaggt aaaatgcgtc gggtcaggca gtcagattct
    16201 taaaatacaa acgtcgtatc cctgaacgga ttgttttctg ttaagttcag gttgtgagca
    16261 taattcgccg gagggatatt aaaatgaatg gaaaattgca aagttcagat gtaaaaaacg
    16321 aaactccgta caatattccc ttattgatta acgaaaatgt gatctccagc ggaatttctc
    16381 tgatctcgct ctggcatacc tacgccgacg agcattaccg ggtgatctgg ccgcgggaca
    16441 agaagaaacc gcttatcgcc aactcatggg ttgcggtgta taccgtacaa ggatgtggga
    16501 aaattctttt aaagaatggc gaacaaataa ctctgcatag caactgtatt atatttttaa
    16561 agccaatgga tattcactct tatcactgtg aaggtttagt ctgggaacag tactggatgg
    16621 aatttacccc caccagtatg atggatattt ccgttggtca gcaaagcgtt atttataatg
    16681 gcgaaattta taatcaggaa ctcaccgaag ttgctgagtt aataacttca ccagaagcaa
    16741 taaaaaataa tctggcagtc gcttttctga cgaaaattat ttatcagtgg atttgtctta
    16801 tgtccgcaga tggtaaaaaa gatccacaac ggcggcaaat tgaaaaatta attgccactt
    16861 tacatgccag tctgcaacaa cgctggagcg tagctgatat ggctgccacg atcccctgta
    16921 gcgaagcctg gttgcgtcgt ctgtttttac gctataccgg caagacgccg aaagaatatt
    16981 acctcgatgc gcgtctggat ctggcgctat cgctattaaa gcaacaagga aactcggttg
    17041 gcgaagtcgc tgatacgctc aacttcttcg actcctttca tttcagcaaa gcctttaaac
    17101 ataaatttgg ttatgcgccg tcagccgtgc taaagaatac ggaccagcac ccaacggatg
    17161 ccagtccaca caattaacgt aactctggcc agtacgcttt gttggcctcc agcaactcat
    17221 ccagaatagc tttggcgacc gaggcgctcg gtaccgtttt cgacaacgta atcgcctgcc
    17281 acaggtgctg atatgaacgc tgttcccagg cgtccacgac cagtttttcc accgccactt
    17341 gctgactcat taaccctttc tgaaactgcg ggatatcgcc gaccaccagt ggttcaggcc
    17401 gtagtagcgc acgcgcatct gtttgcgatc ttgcagccca acaatttgcg ccatccggct
    17461 ttcaataccg attggcatgt cacagatgtt gaggattttc gcattcgggc gcagacgacg
    17521 cgtggcttct gcgacaattg ctgccgggtt ggagtagttg agcatctagg cgtttggtga
    17581 atatttttcc atataatcca ccagttccag gacgccgcca atggaacgca tgccgtacgc
    17641 tattccgccg ggtccgcaag tttcctgacc aacaacaccg tggcgcagtg ggattttttc
    17701 atccagttcg cgcatcgggt acttgccgac gcggatgtgc gccatgacaa aatcaacgtc
    17761 gctgaacgcc acttcaggat cggtggtgta actaaacgca atgtccggcg ctttttcttt
    17821 aaggatgact ttacaggctt cggcaatcac ttcctgccgc gcgccatcgt tatcataaaa
    17881 tttcagtgca cgaagcggga aacggtcctg attcgccagg agcatcaaca cgatgcctgg
    17941 cgtaaaggtg cttccaccgc ctgcgacaac cactgagaat ttggtcatta tactgcctcc
    18001 gtaatggcaa cattttctgc tgattgatga gaattaatta agctatcgag ctgttcacgc
    18061 aattgggata catgcagacc aatgatcacc tgaatggcat cgccactacg gaagacgccg
    18121 tgcgctccca gctttttaaa gacttcgtca tccagcgttt gtgacatgtc atgcagtgca
    18181 atacgtagac gcgtcgcgca attgttaatg ctggagatat tgccgacccc gcccagggct
    18241 tgcaggatac cggcagcctg atccagctct ttttttggct ctgcagcggt ggtttggcct
    18301 cgcgaggctt tgtattcggc ttttgagtag agtttcactt ccgcatcttc acgtcccggc
    18361 gtgcacatgt tgaactgcag aatcagggtg cggaaaacca cgaagtacag cagggtaaag
    18421 cacaacccaa tggcgatttg ggtcagcatc atatccgcgt ggttgctgaa catcgggatc
    18481 cagttttgcg gtaaaacctg gtcaatcaga cctccgccca tgttgcccac cacaccaaag
    18541 agatacatta cggtcgacat tgaggccgcc agcacggcgt gtaccgcaaa cagcaacggt
    18601 gaaatgaaca ggaaggtaaa ttccagcggt tcggtaattc ccaccagcat ggcggttaag
    18661 gttgcgggaa tcagcaagcc cgccactttt acccgatttt ccggtgcggc agtgaagtac
    18721 atcgctaaag aaatgcccac ggcaccaaag atttttgagt taccgtgcag ggcaaaaccg
    18781 ccttccagga acaacgattt cagtggctcg gcgctcagac tgaactcttg cagatgctgc
    18841 gcccagtaca tctgaatgcc gccttcaaca gctgccggac caaagataaa cggtccgtag
    18901 atgaagtggt gtaaaccggt tgggatcaga atacgttcga ggaaggtgta aacccacacc
    18961 ccaagcgcac ccgccgaacg caggaatgct tgcagagatt caatccccat ttgtactttt
    19021 ggccagccga gcaatgtcag ccaggcacag gggatcatca ccaggaaggc gataatcacc
    19081 acataagacg tcccctggaa aatgccgaga aaaaccggca gttttttatc gaacagacgg
    19141 ttatgcagcg ccgtcacaat gccggaaatg ataattgcgc cgataatgct ggtatcgagg
    19201 gttttaatcc cggccatcat tgtcagaccg ctacctgcca ccgcgtcctg agtgaaatcg
    19261 acgccgaagt agcttcccca ggtcattccc atcgcattga tgaaatagtt ccaggtcagg
    19321 aaactcacca tcaccgccag acaagcacgt ccctgcgctt gcttagcaag gccaatgggt
    19381 aaaccgacag caaaaatcag cggcatatta cggaataccg tccaaccgcc ctcttcaata
    19441 atgtgtacga tttgcgcgaa taaactgttc ggatcggtca gtgattcccc gacaaacatc
    19501 gggttttgca gcaagatggc aagacccacc acaatcccgg cgaagggaaa cagcagcact
    19561 ggcgtgaaca tcgcgccgcc aaagcgttga atttgactga gcatttccac atcctctttt
    19621 ctcaattctg taagtaggaa gcctgattgg ttatgcggca gacctggagc gaggatagga
    19681 agatgcgatc ggcagaacat gacgacccaa tgggattcgt gatcgctttc atgcttttcg
    19741 ttgggcggta gagatctacg ttttcgcgaa aaagtagaca tctgtgagca caggtacaca
    19801 gcatcgaaca catctttaaa aaaaagattt ttttcaatcg attaagcaga acttgtgggc
    19861 gcattacccg tgcttgcagg caaaaaagag agctagagat gatctacaaa agcattgcgg
    19921 agcggttaag aattcgactt aattccgcgg atttcacgct aaacagcctt cttcccggtg
    19981 aaaaaaagct ggcggaagag tttgcggtat cgcggatgac tatccgtaaa gccattgacc
    20041 tgctggtagc gtgggggctg gtggtccgcc gccacggcag cggcacttac ctggtgcgca
    20101 aagatgtgct gcatcaaacc gccagcctga ccggactggt ggaggtgtta aaacggcagg
    20161 gaaaaacggt caccagccag gtgctgattt ttgaaatcat gcctgcgcct ccggccattg
    20221 ccagccagtt acggattcaa atcaacgagc agatctactt ctcccgtcgc gttcgttttg
    20281 tggaagggaa accgctaatg ctggaagaca gctatatgcc ggtaaaactg ttccgtaacc
    20341 tttcgctgca acatctggaa gggtcgaagt ttgaatatat tgaacaagag tgcgggattt
    20401 tgattggcgg taattatgaa agcctgatgc cggtgctcgc cgatagactg ctggcgcggc
    20461 aaatgaaggt agcggaacac acgccactgc tgcggatcac ctcgttgtca tatagcgaga
    20521 gcggggagtt tttgaattat tcagtgatgt tcagaaatgc cagcgaatac taggtggagt
    20581 accatttacg gcgactccac ccggaaaaaa gttaaccgat actccagaag agcaccgcca
    20641 gtaattgggg ggtgataatg cgcaggaaca tcactaacgg atagacagtg gcgtaagaga
    20701 gcgccgccgc accactggtt ggatgaagat tattagcaaa cgccagcgcc ggaggatcgg
    20761 tcatggaacc tgccagcatc ccgcacatgg tcaggtaatt cattttggct aacatccgcg
    20821 ccagaatgcc aacagtaatc agcggaacgg cggtgatcag ggcaccataa ccaatccagc
    20881 ttagcccttc gccattgacc agggtattca caaaatcccc accagatttc agaccaacga
    20941 ccgagaggaa cagcacgatc cccagctccc gcagcgcgag gttggcactt ggcggcataa
    21001 accagtacag cttgccgata ctgccgatac gcccgaggat caacgccata atcagcggac
    21061 cgcccgccag ccccagtttc aacgcggccg ggaatcctgg cacaaagacg ggaatagaac
    21121 ctaacaatac gccaagcccg atgccaataa acactggcag catctgaacc tgttgcagtt
    21181 tttgctgcgc attccccagc acattggcaa cggcatcaat tgcggacgga cgccccacca
    21241 ggttgaggat atcgccgaac tgcaggctga tatcgccact ggcgaccagt tcgaccccgg
    21301 cacggttcag gcgcgagata acaacgtcat agcgttcttt aaagtgcagg tcgcgaatac
    21361 gttttccgag cacgttttca ttggtgacca ccacacgctc gacgcgcaaa tcagtgcctt
    21421 tcgtagacag cgaggtatcg acctcctgac caatcaccag ttgcgcatta tgtaaatccg
    21481 ctggctgccc caccagatgc agcaaatcgc ccagttggat aatggtatct ggcgaaggta
    21541 cttttagggt ttcttctcgt ttcagacgcg agcagataat tttgtcgccg ttgagaatcg
    21601 gtacatcttt aatggctaaa tcatgcaggt tagggttctc aacgcgaata ttgatagtct
    21661 tgatcagcgc gccgccattg gtgcgtgaag actcgtgctg ctgagcttct gtctcgacat
    21721 tgacgcggaa aataacccgc aacatccaca tggtgaacaa aatcccgcaa atgccgaatg
    21781 gatacgccat cgcgtaactc atccccatct gatcgaccat ttccattggt gtacccaggt
    21841 cgcgcaagat ctgctgccct gcccccagcg ctggcgtatt ggttaccgca ccggagaaaa
    21901 tccccagcac taccggcagt ggaatatcaa acagtttatg caggatggcg gtaaccagac
    21961 caccgatgat gacgatcaga acagcaaaca ggttgaggcg taatccggag acgcgcaatg
    22021 aggcaaagaa gcccggccct acctgaatcc cgatggtata aacgaacagg atcaggccaa
    22081 attcctgaat aacatgcagc atatcgctac ttaatgtcat ccccgcctga gaaacaaaat
    22141 ggccgacgat gatcccacca aacagcacgc cgccaatacc taatcctatg ccgcgaaatt
    22201 tgacgttgcc gataaacaaa ccgacgactg ccaccaaagc cagaatactg accgttaatg
    22261 ctatatcact catcgtccat ttctcttgag ataacatttt aattgctacg gattatggct
    22321 gaatcggtga agcagatttg ccttatagcg cacattatca cgggaaagaa cgtgccgaaa
    22381 tatcttaaac agtcgaaatt taacgcattg ttgaagaaga aagacaaaaa aagccccacc
    22441 agaatggcgg ggcaaagaga atagctagtt agctatttaa cgcgggacgt tcgctgatag
    22501 cgatacgctg cgctgcgatg ggttcaggct cattacgaat taaatcaata tgcagtaaac
    22561 cgtttacgaa ggttgcgcca gagacttcca tattttcagc cagcgtaaaa ctcaggctaa
    22621 atggctgatt cataagccct tgatgcagcc attttttctc ttcttttggc tgctccggcg
    22681 tgccttttac gctcaggcgc gtgccttcca gttgaatctc taaatcttcc tgacggaaac
    22741 ctgccagcgc aagggtaatg cggtagtggt tatcgtcgct tttctcaatg ttgtacggcg
    22801 ggaagctctg gctttcaccg gcgttttgca gtgcgttggc cagtttgtca aaaccgatcc
    22861 attgacgcat cagtggggat aaatcgaagt tacgcatagt catttctcct tctaagaagc
    22921 gagtaagtac ctgcaaatcc gaagattcgc atatgctccc tgacggcgag catggagatg
    22981 tcaggccgcg ccaggcggcc ttagggaatt agttgatttc gatacggcgc ggttttttcg
    23041 cttccggaat cacgcgttcg agatcgatat acagcaaacc atttaccagg ttagccccac
    23101 gaacatgaat gttctcagct aactggaatt tgcgttcaaa gttgcgttca gcgatgccct
    23161 ggtacagata ggtgcgctct ttttgttcgt cggcgtgagc acctttcacc accagcagat
    23221 tatcctgggc ggtaatttcc agttcgctct cagcaaaacc agccacagcg atagcaatgc
    23281 ggtaatggtt ttcgtctacc agttcaacgt tatacggagg gtagccgcca ttactctggc
    23341 tctggttgtt ttctaagtgg ttaaacaaac ggtcaaatcc aatagcagaa cggtaaagcg
    23401 gggataaatc aaagttacgc ataatcaata gctcctgaaa tcagcgagaa tgtaagacct
    23461 tccacaatgg acaggtcagg tagccagaac acccatcagg cgcgtcctca tcgactacga
    23521 tgtaaaatgg gtctggaaat gaacttttca agccttatca gcggactttt ttttgcagtt
    23581 tatggtctat tgcatagact gagggggcag cacgaaacgt taaagtgcga tagccgccac
    23641 agtgcgacgt aatgatgatg ttattttcag caagggatcg ctataactca tcaggccaac
    23701 acccacgatc aacagatgaa tttattatga taagaaatgt gttgttagcg ttcatgatat
    23761 gcagcggaat gacattactc ggaggatgct ccagcgtgat gtcccatacc ggggggaaag
    23821 aaggaacgta tcccggcacc cgcgccagcg ccaccatgat tggcgacgat gaaaccaact
    23881 ggggcactaa gtcgctggcg attctcgata tgccgtttac cgctgtaatg gacacgcttt
    23941 tactgccgtg ggatgtgttc cgcaaagaca gctcggtgcg ctcgcgcgtt gaaaaaagcg
    24001 aagccaacgc gcaagccacc aatgcggtta ttccaccggc cagaatgcct gataactgat
    24061 ttagcgatca gtttcggtga tccacagctg gccaccttcc atccacgcca gccattgacc
    24121 atccggcgag aagaccacgg cgtccgcaga aggtggattg gcgtggtttt cggttaaata
    24181 ctcaacctcg ccgctttgcg catgggcaca aacaattcga ttatccagca caaagcccaa
    24241 ccattctcct gacggatgcc agttaaatgc agactgaata tccgttttgt tatgggttaa
    24301 cttgcgcggt tcgccgccct gtggcgagat aagccacagt tgcacaatgc cgttatcatc
    24361 acgcattaaa aacgcgattt gcgtaccctg cggattacag cgcacccagt ggcgcgggac
    24421 gttgactaac cccggataag cccgatgatg ggtaaaggtt aaacgtcgct gcacgacgcc
    24481 acgcggtggc gcgggtaggg ttgtttccgt tccacttaac ggcgcatcac ctgccgattt
    24541 ccagctagct tcatcttgcg gtaactcaac gataaatagc tccggcactt tctcgccctt
    24601 tggcgaaagt gtgtcgccaa taaacgccag cgcgtgattt cctacccatc cttcttcata
    24661 agcacgattg atttcatcac tgccaggctg cggcgtgggc gtggttttac tcaccagcac
    24721 acaccagtgg ctaccgctgt attcacgcgg atgttgcttt tgtacgttga ccgggccaaa
    24781 cggtgcagca accccgacgt ttcgcaaatc cagcgccgga tcgagttgat gcattacatg
    24841 gtcgttatag gtaaagctcc ccctttcacc gttcgggcta aagacatgca catggctgcc
    24901 accgcgcagc gctcctggcg tatacggcgc ggtaatatcc attgcatcga gattgctcac
    24961 cttgccgcct tcagcaatga ctccgcgtcg atgatggaag tcgtaatgcc atgtttcatc
    25021 aggattttca gggccgtgaa taaagacata tttctctgac ttcggatgaa cggtcaccac
    25081 gccgacgtgt gcgccctgtg acgcgcgata gataacctcg acctcgcctg tatggatatt
    25141 cacacgctca atggtctcgc cggtaaacga cgccccagaa ggacgcacgt caaataccag
    25201 ccactggctg tcgggcgtcc aggtattggt attggtattg gtattggtat tggtattggt
    25261 gagcaggtga ttacggggag caaaggttat ctgtttcatg gcgcggtcct gatacaaaac
    25321 ggttcccgcc atcatacttc acaaggactt cactttcaga caatagtctt ttgccatcgt
    25381 gatgaatgtg agtgaaaaga tggaacattt cgacgtggcg attattggcc tcggcccggc
    25441 agggtcggcg ttggcacgaa agttagccgg caaaatgcag gtgatcgcgc tggataaaaa
    25501 gcaccagtgc ggtactgaag gtttcagcaa accttgcggc ggtctgctgg caccggacgc
    25561 gcagcgatct tttattcgcg atggactgac gcttcctgtc gatgtgatcg ccaatccaca
    25621 gattttcagc gtcaaaactg tcgacgtcgc cgcatcgctc acgcgtaact accagcgaag
    25681 ctatatcaat attaatcgcc atgctttcga cttgtggatg aaatcgctga tccccgccag
    25741 cgttgaggtt tatcacgata gcctgtgccg aaaaatctgg cgtgaggatg ataaatggca
    25801 tgtcattttt cgtgcagacg gctgggagca acatattacc gcccgctatc tggtcggtgc
    25861 cgatggcgca aactcgatgg tgcggcgaca tctctacccg gatcatcaaa tccgtaaata
    25921 tgtcgctatc cagcagtggt tcgcagagaa acatccggtg ccgttctact cctgcatctt
    25981 tgataatgcg ataactgact gttactcatg gagtatcagc aaagatggtt attttatctt
    26041 tggcggtgcc tatccaatga aagacggtca gacgcgtttc acgacgctga aagagaaaat
    26101 gagcgccttt cagttccagt ttggcaaagc ggtgaaaagt gagaaatgca cggtgttgtt
    26161 tccctcacgc tggcaggatt ttgtctgtgg taaggacaac gcctttctga ttggtgaagc
    26221 ggcgggattt atcagcgcca gctcgctgga ggggattagc tatgcgctgg atagcgcaga
    26281 gattctgcgt gcggtgttcc tgaaacagcc ggagaagagc aacgccgctt actggcgcgc
    26341 cacacacaaa ctgcgtttaa aactcttcgg caagatagta aaaagccgat gcctgaccgc
    26401 accggcttta agaaagtgga ttatgcgcag tggtgtggcg catattccac agttgaaaga
    26461 ttatccaacg cgcttcacat cacccaccag caggatgtaa gagagcgcgc caatcaacgc
    26521 gacggcggag atataaacca gtgcaggcgc gaaaccgtaa ccctgcgcca ggtaccccac
    26581 caccagcgga acggtgatgc cgcccagacc accggcgaag ttaaacacgc cgccggttaa
    26641 accaatcagg cgcatcggtg ccagagaaga gaccagcgac caggtaatcg aagcaaaacc
    26701 gttgccgaag aatgccagcg ccatcaggca cataatcatc atcggatcgt tggtgtagtt
    26761 agcgcccata atgcaggtgg agatcagcaa gccgcagata atcggcgttt tacgcgcaaa
    26821 gcccagtgaa aagcccttac gtaccagcag atccgcgacc cagccagaga gcaggacgcc
    26881 gacaaacgcc gcgaggaatg gcaccgtggt cataaagcct gctttcagcg ccgtgattcc
    26941 tttttcctgg gttaaatagt tcgggaacca ggttaagaaa aaacacagtg cagaagccac
    27001 cgcaaattgc ccaagataaa cgccgatcag tttacgatgg aacaccagtt tccagtcttt
    27061 ggctgttaac ggctgacgcg cctctttctt caccggcgca tcaccatcca ccagaccgcc
    27121 gccatcacga atgtaatcca gttcagcttt gctgatacct ttggtcaggc gcggcggctg
    27181 ataaacctta aaccaaatca gcgaccagat aatgccgata ccaccagtga caatgaacac
    27241 ccagtgccag ctcaacatct cctgaatcca gatcagcagc ggagtcaaaa acgccagacc
    27301 gacaaactga ccagacgtat aaaaaccaac ggcggaagcg cgttcatgtt ccgggaacca
    27361 gctggtcacc atccggttat tggtcgggaa agcgggcgct tcgaaaatac cggttatcgc
    27421 acgcagacca attaatgaca ttaatcccgt ggcaaagcct tggaataaag tcgccactga
    27481 ccaaccaaat atcgcaataa aataagtcac gcgagaacct acgcgatcta aaaaccaacc
    27541 gccggggatc tggcatagcg tataaagcca ggcgaaggcc gaaaatacat agcccatttc
    27601 cgctttcgta atgccgaact cttcctgaat atgggcggaa gccacggcca ggttagcgcg
    27661 gtcaacataa caaatgacca ccgtaataaa gatcatcacc agcgtcagat aacgccgacg
    27721 ccccggcttt gctgcattaa cgggaatatc catagcgagc tttctccaga ttttgggcat
    27781 agcgaagccg ctcaccatgc cctgtaattt acagagggtt atatttttgt attgctgttt
    27841 tagtgcccga tgaggggctt acgtggcagg aattaccact ctgctacgct gttatcttca
    27901 tgacgccaga gcggattacg ccagtccggg gcatttttac tgaactcaat aactttagct
    27961 tcgtcgattt ccacacctaa gcccggtttc gttaacggtt taaagaagcc gccaaccatg
    28021 ctgaagtctt ctttgttttt cacaaagtcg agtaactccg cgcctttgtt gtaatgaatg
    28081 cccatacttt gttactgaag taccgcgtta taggaaacaa agtcgatatg caggcaagcc
    28141 gccagtgcaa tcggtccgaa cggacagtgc ggcgcaaggg tcacatcata ggcttctgcc
    28201 attccagcaa ttttgtagca ttcggtaata ccgcctgcat gggagagatc cggttgcaga
    28261 atcgaaatac cgcctgcctc cagcacgcgt ttaaaatcga aacgtgagaa catgcgctca
    28321 cccgccgcca gtggaatatg cgtttgtgcc gccaatttcg ggtagtattc ggcttgttcc
    28381 gccagcaccg gctcttcaat aaacagcggg cgatacggct ccagttcttt aatcagcact
    28441 ttcgccattg gcgcgctgac gcgaccgtgg aaatcaagac caaactcaat ctgattgcca
    28501 aaagcttcac gaatttgtgc cacggtgttg actgccgcat ctaccgcgcg ggagttatca
    28561 attagcccca gttcttcaca accgttcagt ttgaaggtat cgaagccgat ttcgcgcagg
    28621 gttttaatgc cgtcgataac atccgccgga cgatcgccgc cgacccaact gtaggcttta
    28681 attttgtcgc gaaccaggcc gcccatcagt tgccagactg gcgcattcag cactttgcct
    28741 ttgatatccc ataacgcctg gtcgatcccg gcgatggcgc tcatcaggat tggaccgcca
    28801 cgataaaatc cggcgcgata catcacttgc cataagtcgt tgatgcgcga aggatcctga
    28861 ccaatcaaat agtcacccag ctcgtgaact gccgcttcca ccgtacgggc gcggccttca
    28921 atcacgggct cgccccaacc gaccacgcct tcatcggttt caattttcag gaacatccag
    28981 cggggaggta aacgatacgt ggtaattttg gtgattttca ttgcactgcc tctcgatacg
    29041 ccttaacaaa tgctgctgcc tgctgcgcgg tgcgttctac ggactgcccg gcgcgataaa
    29101 gatcgctgcc taagcccgcc ccaacacaac ctgcgtctat ccactgcgcc aggttttctg
    29161 gcgtcacgcc gccaacggca aagactgcga tgtccggtgg taataccgct tttaacgctt
    29221 tgatgtattg cggaccaaaa gccgatgacg gaaatatttt cagcgcctgc gcgcccgctt
    29281 cgagcgcggt aaaggcttcg gtcgccgtcg cgcagccggg gcaaacggtc atgccgtagc
    29341 ccaccgcgcg gcggatcact tcactatgga tattgggcgt aacgatgagc tgacagccca
    29401 tcctggcgag cgcatcgacc tgttcaggtt tcagtaccgt acctgcgcca atcaacgcct
    29461 tgtcgccata cgcatcaacg atggcgggaa tgctctgctc ccattgtggg gaattcagcg
    29521 ggatttcaac cgcgtcgaac ccggcgtcaa tcaccgcgcc aacatgcgcc agcgcctcgt
    29581 cgggcgtaat gccgcgcaaa atggcgatca gcgggagttt agtttgccac tgcatgagcg
    29641 atgctcctta taccagcctg aaatgccgtg tcgcccgcca ccgccgccac gtcgcaaccc
    29701 atcgcctgaa aggcttgctg gtagcgcgcg gtcagcgatg ttccggcgac aagggtgatg
    29761 gcgtgttgat gggtcacata gtcgcgcata ctggcaacct ctgcgccaat caacaaacca
    29821 gagagaaatt cgctgacctg ttcgcgggga agtgttccca gcacatgcga ggcgcgaact
    29881 tcaaaaagct gcggcaatat ggcgggcgca ttaaggccgc gctcaaggcc agccgcgaag
    29941 gcatcggcag agttttcctg cggcggcaaa cctgcgccaa tcaatgagtg atttaacagt
    30001 aaatgatgta attcaccggt catcacggtg cgaaaatcgt tgatttgctg gctatcggcc
    30061 tgcacccatt tgcaatgcgt accgggcata acataaagag aggaaggagc cagagctcgc
    30121 gcgccgatca attgtgtttc ttcgccgcgc atcacattgt ggttatcgtc atgagagacg
    30181 cataatccgg gaataatcca gatattgtcg ccaacagacg ttaattgttc gccaatagac
    30241 gaaaaacggg caggaacaga taaatacgga gcaactttcc agccgacatt gctgccgacc
    30301 attcctgcca tcaccactgg cgtattttct tcacgccagt cggtcgtgac ttctgctaac
    30361 actgcagccg gagattttcc gttcaggcgc gtgacgcctg cttctgattg cctgctctcc
    30421 aggcagtggt cgccctgata aagccaggcg cgcagattgg tcgatcccca gtcaattgcg
    30481 atgtagcgag ctgtcatgtg atttccttta accttcgtgt cgagctggca atcatggtaa
    30541 gcgccgcctg ctctgccgca tcgccgtcct gatgccgtat cgcatcgaac agcgccttat
    30601 gttcctggag cgtttgcggc atgttggcct catcgcccat ccaggttcgt tcaaaaaccg
    30661 cccgctgcag cgaactgatc gcaatgctaa gttgctgtaa caccgggtta tgcaccgact
    30721 gcagcaccgc ctcgtggtag cgaatatccg cttcgttaaa cgcttcgcgg tcctgattgt
    30781 tggcaatcat ctcgttcagc gccgattcaa tctgcgccag atcgctggag gtcgcgcgct
    30841 ctgctgccca acgggcaatc gccggttcca ccagatttcg cacttcgctc atggcactga
    30901 taagccgtgg gtcgtagtcg ttttccagca cccattgcag tacgtcagtg tcgaggtaat
    30961 tccactggtt acgcggtgcc acaaacgccc cgcgataacg tttcatttca atcagccgct
    31021 tcgccatcag cgaacggaac acctcacgga tgatgttgcg cgaggttgca aactcttcac
    31081 agagctccgc ctcagccgga agtggcgagc caggtacgta tttgccgtga acgatctgtt
    31141 tacccagcgt aatgacaatg cgatcggttt tattgagagt catggagagt ccttgtgctc
    31201 tgtatgttct tctctacttt accccgatcg atgcataacg cggcaacttt gtagtaccag
    31261 cgtgatgacg ttcgcgtttg ccgcgcgcgt aatgtagtac aaacttatat tattgcgcta
    31321 caatttagat caaaaaaaga acaacacata aaaaaatgac atgcgtcggg cagaaatctg
    31381 aaaagggata tcaggcgcta aacaggaggg aaagaagagt atgctttcaa cggcttagct
    31441 actcgtttaa aggattaatc atgaagttga attttaaggg attttttaag gctgccggtt
    31501 tattcccact ggcgctgatg ctttcaggct gtatctcgta tgctctggtt tcccataccg
    31561 caaagggtag ttcaggaaag tatcaatcgc agtcagacac catcactggg ctatcgcagg
    31621 caaaagatag taatggaaca aaaggctatg tttttgtagg ggaatcgctg gattacctta
    31681 tcactgatgg tgccgatgac atcgttaaga tgctcaatga tccagcactt aaccggcaca
    31741 atattcaggt tgccgatgac gcaagatttg ttttaaatgc ggggaaaaag aaatttaccg
    31801 gcacaatatc gctttactac tactggaata acgaagaaga aaaggcactg gcaacgcatt
    31861 atggttttgc ctgtggtgtt caacactgta ccaggtcact ggaaaaccta aaaggcacaa
    31921 tccatgagaa aaataaaaac atggattact caaaggtgat ggcgttctac catccattta
    31981 aagtgcgatt ttatgaatac tattcaccca gaggcattcc ggatggtgtt tccgcagcat
    32041 tactgccagt gactgttaca ctggacatca ttactgcacc gctgcaattt ctggttgtat
    32101 atgcagtaaa ccaataatca gtaagcgggc aaacgcgttt atgctgtttg cccgcccaca
    32161 gattaattca gcacatactt ctcaatagca aacgccacgc catcttcaag gttggatttt
    32221 gtgacaaagt tcgccacttc tttcaccgaa ggaatagcgt tatccatcgc cacaccgacg
    32281 cctgcatatt caatcattgc gatgtcgttt tcctgatcgc caatcgccat gatttcttcc
    32341 ggtttaatac ctaacacgtc ggccagtgat ttcacacccg tacctttgtt aacgcgttta
    32401 tcgaggattt cgaggaagta cggcgcactt ttcagcacgg tatatttctc tttcacttcc
    32461 tgcggaatac gcgcgatagc ctggtcgagg atggcgggtt catcaatcat catcactttc
    32521 aggaactggg tattggggtc cattttctcc gcttcgcaga acaccagcgg aattgtggca
    32581 acgaaggatt catgcaccgt gtagtagctg atatcgcggt tagcggtgta cagcgtggtg
    32641 cggtccaggg cgtggaaatg agaaccgact tcgcgagaga gtttttccag gaaacgatag
    32701 tcgtcatagc tgagagcggt ttgcgccacg gtgctaccat cagcggcctt ctgtaccagc
    32761 gcgccgttat aagtaatgca gtagtcgccc ggctgttcca tatgcagctc tttcaggtag
    32821 ttgtgcacac ctgcatacgg acgacccgtc gttagcacga cattcacgcc acgggcgcga
    32881 gctgcggcaa tcgcattttt aacggcgggt gaaatggtgt gatcgggcag cagaagggtg
    32941 ccatccatat cgatagcaat gagtttgata gccatgagtt cccaaggtag attagttcct
    33001 gacccatgct aacgcgattc cgctcaaaaa tcagtacaac acccgaggga aaaggggatg
    33061 caacgcgggt tgcatgctcc ccatttgctt agcggaagag tttccctttc agcagttcca
    33121 tgcctgcgga aagcagatcg ttattggctt gtgctgacac ttcaccttgc ggtgagagcg
    33181 catcaataat cttcggcaat tgctctgcca gtaaactgga agccgtactg gtatccacgc
    33241 caagtttttg cccgagatcg gacaccgcat ttgtgccgag cgccgactcc agttgctcgc
    33301 cgctaactga ttgatttctc tgttgattac tcaaccaggt tgagagaatg gcccctaagc
    33361 cgccactttg cagtttttcc agcagcacct gaatgccacc ctgctcctca acccaactta
    33421 aaatcgcctg atatttcccc gcatcgcctt tcagaaaggc accgacaact tcatcaaaaa
    33481 gccccatgat aatcacctgt aaagcgttac gtgttgaccc aaaaagtata gatctgcggg
    33541 atgataattg cggattgcag aaataaaaag ggcggagatg atctccgccc ttttcttata
    33601 gcttcttgcc ggatgcggcg tgaacgcctt acccggccta caaaatcatg caaaatcaat
    33661 atattgcagg agtctcgtag gactaataag cgtagcgcat caggcacgct cgcatggtta
    33721 acgccattaa atatcaatat tcgccgcttt cagggcgttc tcttcaataa acgcacgacg
    33781 cggttctacg gcgtcgccca tcagcgtggt gaacaactgg tcagcggcaa tcgcatcctt
    33841 aacggtaacg cgcagcatac gacggctttc cgggtccatg gtggtttccc acagctgttc
    33901 cgggttcatc tcgcccagac ctttataacg ctggatggag aggccacgac gggactcttt
    33961 caccagccag tccagcgcct gctcgaagct ggctaccggc tgacgacgct cgccacgttc
    34021 gataaacgca tcttcttcca gcaagccacg cagtttctca cccagcgtgc agatacgacg
    34081 atattcgcca ccggtgataa actcgtgatc cagcggatag tcagtatcca caccgtgggt
    34141 acgcacgcga acaatcggct cgaacaggtt ttgttcggca ttggtgtgga catcaaactt
    34201 ccactggctg ccgtgctgtt ctttgtcgtt cagttcgctg accagcgcgt tcacccagcg
    34261 ggtaacggtc tgctcatcag aaaggtcagc ttccgtcaac gtcggctgat agataagctc
    34321 tttcagcatt gctttcggat aacgacgctc catgcggttg atcattttct gcgtcgcgtt
    34381 gtactcagat accagtttct ctaacgcttc gccagccaat gccggtgcac tggcgttggt
    34441 gtgcagcgtt gcgccgtcca gcgcgataga gatctggtac tgatccatcg cttcgtcgtc
    34501 tttaatgtac tgttcctgct tgcctttctt cactttgtac agcggcggct gagcgatgta
    34561 gacgtgaccg cgttcaacga tttccggcat ctgacgatag aagaaggtca acagcagcgt
    34621 acgaatgtgc gagccgtcga cgtccgcatc ggtcatgatg atgatgctgt gataacgcag
    34681 tttgtccggg ttgtactcgt cacgaccgat accacagcca agcgcggtga taagcgtcgc
    34741 cacttcctga gaagagagca tcttatcgaa gcgcgctttc tcgacgttaa ggattttacc
    34801 cttcagcggc agaatcgcct ggttcttgcg gttacgcccc tgcttcgcag agccgcccgc
    34861 ggagtcccct tccaccaggt acagttcgga aagcgccgga tcgcgttcct ggcagtctgc
    34921 cagtttgccc ggcaggccag ctaagtcgag cgcaccttta cggcgggtca tttcacgcgc
    34981 gcgacgcgcc gcttcacggg cacgggcagc atcgataatt ttgccaacca cgattttcgc
    35041 atcagttggg ttttccagca ggtattccgc cagcagttcg ttcatctgct gttcaaccgc
    35101 cgatttcacc tcagaagaaa ccagtttgtc tttggtctgg gaggagaatt tcgggtccgg
    35161 cacttttacg gaaacgaccg caatcaggcc ttcacgcgca tcgtcaccgg tggcgctgac
    35221 tttggctttt ttgctgtagc cttctttgtc catgtaggca ttcagggtac gggtcatcgc
    35281 cgcacggaag cctgccaggt gagtaccgcc gtcacgctgc ggaatgttgt tggtaaagca
    35341 gtagatgttt tcctggaagc catcgttcca ctgcagcgcc acttcaacgc caataccgtc
    35401 tttttcggtg gagaagtaga agatatttgg gtggatcggc gttttgttct tgttcagata
    35461 ttcaacgaac gctttgatgc cgccttcata gtggaagtgg tcttctttgc cgtcacgctt
    35521 gtcgcgcaga cgaatggaaa cgccggagtt gaggaacgac aactcacgca gacgtttcgc
    35581 cagaatttca tattcgaact cggtcacatt ggtgaaggtt tcgaggctgg gccagaaacg
    35641 caccatggtg ccggtttttt cagtctcgcc ggtaaccgcc agcggagcct gcggtacacc
    35701 gtgttcgtag atctgacggt gaattttacc ctcgcgctga ataaccagct ccagtttttg
    35761 cgacagggcg tttactaccg aaacaccaac gccgtgcaga ccgccggaca ctttatagga
    35821 gttatcgtca aatttaccgc ctgcgtgcag aacggtcatg atcacttccg ccgccgatac
    35881 gccctcttcc gggtgaatac cggtcggaat gccgcgcccg tcatcctgta cagagacaga
    35941 gttatcggcg tgaatggtga cgataatttc tttacagtga cccgcgagcg cttcgtcgat
    36001 agcgttatct accacctcga ataccatgtg gtgcagaccg gtgccgtcat ccgtgtcgcc
    36061 gatatacata cccgggcgct tacgcaccgc atccagccct ttcaggactt tgatactgga
    36121 ggagtcataa gaattcgaca tcaacgtttc tcgctcattt atacttgggt taatccgtta
    36181 ttttaccctt ttccacggta aacatcttcg aattttcgtc cgacatgtct ataacgtgtt
    36241 cagcactgat cgcgctgaca aagacctgtg attgcgtcgc ttttaagcgg ctggcaagca
    36301 acccgcgacg ctcatcatca agctcagagg caaaatcatc tatcaggtag agacaccgcc
    36361 gcccgctttc acgagtgagg aactctcctt gcgccagacg taaggcgcac atcaacagct
    36421 taagctgccc acgcgataag gtatcttcca ccggcgcacc gtcggcgcga atgcgtaagt
    36481 ccgctttatg cgggccatgc gcggtgtagg ttagctggcg atcgcgttca aaattacgtt
    36541 ccagcacctc agcgtactct gtctctttct cccagccgcg ctggaaagag aaagtcagag
    36601 aaaactcagg gagaaattgc ttacaggtat catccatatc ggccgcgata ccggcgctat
    36661 actccgcgcg ccaggtgctg atttgctccg ccagcgggat cagttcttta tcccacgggc
    36721 gtagctgttc gtaacgagtc acctggcgca gcgccgcatt gcgctgcttg agcaatcgct
    36781 tgagattgct ccaggcggtg aaaaatccag gttcgttgtg aaagcatccc cagtcgagga
    36841 atgctcttct gtatttgggg ccgccgttga gtaaagtaaa cccttctggc gttatcaact
    36901 gcatcggcat caggtgcgcc agttccgcga ctttatgccc gtccgtaccg tcaatacgga
    36961 ctttgctgtc gccctgtttg tctttggtta agccaatcgc cgtctcgcgc tcttcgccct
    37021 gtaatcgccc gtggagaaca aatgcctcct gctcatggcg aatgacgcga ccaatctgca
    37081 aactgcgaaa cgcccgacca tggccgagcg tatagatggc ttccagcacg ctggttttgc
    37141 cactgccgtt ggcacctacc agaaagttaa agccgggaga taaagcgaga tccgcggttt
    37201 caatattgcg gaaatcgcgg atcaacaagc gggtgaggga cattacagtc tcattggcat
    37261 gacaacatag gcagccgact gtgatgcggc atcttcaatc tgcacgctgg aaaccgaatc
    37321 ggtcagcatc atgcggacgt tttcgcattt cagcgcgttc agaacatcca gcacatagct
    37381 gacgttgaag ccgatttcca tctccgcacc gctataggta acgtcgagga tctcttccgc
    37441 ttcttcctgt tccgggttgt tggcagtaat tttcagctgg ttttcgctga catacaggcg
    37501 cacaccgcgg aatttctcgt tagagagaat cgccgcgcga gcaaacgcct gcttgagcag
    37561 atcgcagcct gcttccagat gtttgtccgg gttcttcggc agaacgcgac ggtaatccgg
    37621 gaagcgacca tccaccagtt tcgaggtgaa aataaagtcg ccaacgtggg cacgaatgtt
    37681 attgctgcca atctgcacac gcagcagatt gtcgccgccg tcgagcatac gcatcagttc
    37741 aatcacgcct ttacgcggta cgatcaccga atggcttggc aaagattgac caattggcat
    37801 tgaacagacc gccagacggt ggccgtcggt tgccacggtg cgcagttctt caccttcggt
    37861 ttcaaacagc ataccgttta agtaatagcg aacatcctga tgcgccatcg aaaactgggt
    37921 tgcttcaatc agacgcttca tcgtcgcctg cggcagggta aattcgactt cgctctgcca
    37981 gtcatcgagg ttcgggaaat ccgccgctgg cagggtagac agcgaaaaac ggctacgccc
    38041 ggagcgcacc agcatccgtt caccttccag ctgcacggca atttccgcgc cttcaggcag
    38101 accacggcag atatcaaaga atttgcgcgc cggaacggtc gtcgctcctg gctcgtgtgg
    38161 ctgaaccagc gcaacgcgtg ccaccatttc catctcgaga tcagtaccgg tcagcgacaa
    38221 cgtaccgtca gcaacctgta acagcagatt accgagaatc ggtagcgtag gacgaccacc
    38281 taacggaccg ctcacctgtt gtagcggttt taataaatgc tcacgttcta cggtaaattt
    38341 cataggttta cgatgacaat gttctgatta aatttgaaaa atcttctttg atatcgtggc
    38401 tctcttcacg caactgctcg atcttacggc aggcatgaag caccgtcgtg tggtcacgcc
    38461 caccaaacgc atcgccaatc tctggcagac tgtggttagt cagctctttc gctagcgcca
    38521 tcgccatctg gcgcggacgc gccaccgagc gggatcgacg cttggaaagg agatccgcga
    38581 ctttgatctt gtagtactcc gccaccgtct tctgaatatt gtcgatggtg accagttttt
    38641 cctgcaatgc cagcaagtcg cgcagcgcct cacgcacgaa gtcgatggtg atcgcccgtc
    38701 cggtaaagtt ggcgttagca ataacgcggt tcagcgcccc ttccagctca cgtacgttag
    38761 atcgtagacg cttggcgata aagaacgcca cttcacccgg caaacgaatg tcattttcgt
    38821 cggccttttt catcaggatc gccacacggg tttccagctc tggcggttcg atcgccacag
    38881 tcagtcccca accgaagcgg gatttcaaac gatcctcaac gccgttgatc tctttcggat
    38941 agcgatccga ggtgagaatg atctgttgat taccttccag cagggcgttg aaggtgtgga
    39001 aaaactcttc ctgagatcgt tctttattag caaaaaactg aatatcgtcg atcagcagtg
    39061 catctacgga acggtagtag cgtttaaact cttcgatcgc gttgttttgc agggctttaa
    39121 ccatgtcctg aacaaagcgc tcggagtgca tataaaccac tttggcattc ggcttgcgcg
    39181 ccataatgcc gttacccacc gcatgcagca ggtgagtttt acccagaccc gtgccgccat
    39241 aaaggaacaa cgggttatag gcaccgccag ggttatccgc cacctggcaa gccgccgcgc
    39301 gcgccagttg gttagattta ccttcaacga agttatcaaa cgtgtgtttg acgtttacgt
    39361 tagaacgata ggtcggttct gccggggccg ggacgttatc ccaacctgag cgcgtagaag
    39421 gcgcagcacg ttgcggctgc gtttgcgcca cctgtgcagg ggccgcgacg ttgctcgtca
    39481 ctgccgcttg tggcgtttgc gtcaccggtt tggtgccgac ttcaaaacgc agctgtgggg
    39541 catccgctcc gcagaaactg gttagcagtc cattgatatt attaaggtac ttgtcccgta
    39601 cccaatcgag gacaaaacgg tttggcgcgt acagggccag cgtgttatcg ctcagttccg
    39661 cctgcaatgg gcgtatccac atactgaatt ctgtggctgg taactcatcc tgcaatcggg
    39721 caagacactg ctgccaaagc gaaagtgaca cggcggactc cactcgaaca aaagtcgata
    39781 atgactaagg ctgaaacatt catgattgtt gacgtacgtc gaaaagaccc tgcttgaggg
    39841 tgacgcacga accgctgtct gcggttatat gcccgatcaa gatcctgcaa aacgatcggg
    39901 accgcggatc atagcctaaa ctgcgcaaga gatcttctgt ttctcacaga ttttccctat
    39961 ttatccacag gactttccag aactcgctaa gtgtaaacga tcctgccgcg aggcgggcac
    40021 gatttacgcc gcatattgga aaatttaatg accatagaca aaaattggct taatcgatct
    40081 aataaagatc caggacgatc cttgcgcttt acccatcagc ccgtataatc ctccacccgg
    40141 cgcgccatgc tggtttccac tggtgtgagg tcgtacattt tccctgcgaa aaggtgcgga
    40201 aaagcgcggt aaataaggaa agagaattga ctccggtgtg tacaattatt acaatccggc
    40261 ctctttaatc acccatggct tcggtgtcca tcgtttcatt tttcggcgga tatccaataa
    40321 agccattgaa tttattcaag tttaggtaga aatcgccatg aaacgcactt ttcaaccgtc
    40381 tgtactgaag cgcaaccgtt ctcacggctt ccgtgctcgt atggctacta aaaatggtcg
    40441 tcaggttctg gcacgtcgtc gtgctaaagg ccgcgctcgt ctgaccgttt ctaagtaata
    40501 aagctaaccc ctgagtggtt aagctcgcat ttcccaggga gttacgcttg ttaactccca
    40561 gtcaattcac attcgtcttc cagcagccac aacgggctgg cacgccgcaa attaccattc
    40621 tcggccgcct gaattcgctg gggcatcccc gtatcggtct tacagtcgcc aagaaaaacg
    40681 ttcgacgcgc ccatgaacgc aatcggatta aacgcctgac gcgtgaaagc ttccgtctgc
    40741 gccaacatga actcccggct atggatttcg tggtggtggc gaaaaaaggg gttgccgacc
    40801 tcgataaccg tgctctctcg gaagcgttgg aaaaattatg gcgccgccac tgtcgcctgg
    40861 ctcgcgggtc ctgatagccc tcattcgggt ctatcaacgc ctgattagtc cgctactcgg
    40921 gccgcattgt cgtttcactc caacctgttc aagctacgga attgaggcat tgcgcaggtt
    40981 tggagtgata aaaggcagtt ggttgacggt gaaacgcgta ttaaaatgcc accctttaca
    41041 ccctggtggt gacgatcccg tcccgcccgg accatttgat accagagaac actaacgatg
    41101 gattcgcaac gcaatctttt agtcatcgct ttgctgttcg tgtctttcat gatctggcaa
    41161 gcctgggagc aggataaaaa cccgcaacct caggcccaac agaccacgca gacaacgacc
    41221 accgcagcgg gtagcgccgc cgaccagggc gtaccggcca gtggccaggg gaaactgatc
    41281 tcggttaaga ccgacgtgct tgatctgacc atcaacaccc gtggtggtga tgttgagcaa
    41341 gctctgctgc ctgcttaccc gaaagagctg aactctaccc agccgttcca gctgttggaa
    41401 acttcaccgc agtttattta tcaggcacag agcggtctga ccggtcgtga tggcccggat
    41461 aacccggcta acggcccgcg tccgctgtat aacgttgaaa aagacgctta tgtgctggct
    41521 gaaggtcaaa acgaactgca ggtgccgatg acgtataccg acgcggcagg caacacgttt
    41581 accaaaacgt ttgtcctgaa acgtggtgat tacgctgtca acgtcaacta caacgtgcag
    41641 aacgctggcg agaaaccgct ggaaatctcc tcgtttggtc agttgaagca atccatcact
    41701 ctgccaccgc atctcgatac cggaagcagc aacttcgcac tgcacacctt ccgtggcgcg
    41761 gcgtactcca cgcctgacga gaagtatgag aaatacaagt tcgataccat tgccgataac
    41821 gaaaacctga acatctcttc gaaaggtggt tgggtggcga tgctgcaaca gtatttcgcg
    41881 acggcgtgga tcccgcataa cgacggtacc aacaacttct ataccgctaa tctgggtaac
    41941 ggcatcgtcg ctatcggcta taaatctcag ccggtactgg ttcagcctgg tcagactggc
    42001 gcgatgaaca gcaccctgtg ggttggcccg gaaatccagg acaaaatggc agctgttgct
    42061 ccgcacctgg atctgaccgt tgattacggt tggttgtggt tcatctctca gccgctgttc
    42121 aaactgctga aatggatcca tagctttgtg ggtaactggg gcttctccat tatcatcatc
    42181 acctttatcg ttcgtggcat catgtacccg ctgaccaaag cgcagtacac ctccatggcg
    42241 aagatgcgta tgctgcagcc gaagattcag gcaatgcgtg agcgtctggg cgatgacaaa
    42301 cagcgtatca gtcaggaaat gatggcgctg tacaaagctg agaaggttaa cccgctgggc
    42361 ggctgcttcc cgctgctgat ccagatgcca atcttcctgg cgttgtacta catgctgatg
    42421 ggttccgttg aactgcgtca ggcaccgttt gcactgtgga tccacgacct gtcggcacag
    42481 gacccgtact acatcctgcc gatcctgatg ggcgtaacga tgttcttcat tcagaagatg
    42541 tcgccgacca ctgtgaccga cccgatgcag cagaagatca tgacctttat gccggtcatc
    42601 ttcaccgtgt tcttcctgtg gttcccgtca ggtctggtac tgtactatat cgtcagcaac
    42661 ctggtaacca ttattcagca gcagctgatt taccgtggtc tggaaaaacg tggcctgcat
    42721 agccgcgaga agaaaaaatc ctgattcggt gagttttcgc taaaataagg gcggtcagtt
    42781 gaccgccttt tttcttttcg tagggcggat aagcaccgcg catccgccac acaaagcaac
    42841 aggaacatca tgagcgataa tgacactatc gtagcccagg ccacgcctcc gggacgtggc
    42901 ggcgttggca tcctgcgcat ctccggcttc aaagcccgtg aagttgccga aaccgtgctg
    42961 ggtaaactgc ctaagccgcg ctacgccgat tatcttccgt ttaaagacgc cgacggcagc
    43021 gtgctcgatc aggggattgc gctatggttc cctggcccga actcgttcac cggcgaagat
    43081 gtgctggaac tgcaaggtca tggcggtccg gtgatcctcg acctgctgtt aaaacgcatt
    43141 ctgaccattc ccggcctgcg gattgctcgc cctggtgagt tttccgaacg cgcgtttctt
    43201 aacgataaac ttgacttagc ccaggccgag gcgattgccg atcttatcga cgccagttcg
    43261 gaacaggcgg cccgttcggc acttaactcg ctgcaaggcg cattctccgc acgggttaat
    43321 catctggtag aagccctcac ccacttgcgc atttacgtcg aagcggcaat tgatttccct
    43381 gatgaagaga tcgatttcct ctccgacggc aaaattgaag cccagcttaa tgacgtgatg
    43441 gccgatctcg atgcagtgcg tgctgaagca cgtcagggta gtttgttgcg cgaagggatg
    43501 aaagtagtga ttgccggacg tcctaatgcc ggtaaatcga gcctgttaaa cgcgctggcg
    43561 ggccgtgaag cggcaatcgt aaccgatatc gccggaacca cgcgtgacgt gctgcgtgag
    43621 catatccaca ttgacggaat gccgctgcat atcatcgaca ctgccgggct acgtgaggcc
    43681 agtgacgaag tggaacgtat tggtatcgag cgcgcgtggc aggaaattga acaggccgac
    43741 cgcgtgctgt ttatggtcga tggcaccacg acagacgccg ttgatccggc agagatctgg
    43801 ccggaattta tcgcccgtct gcccgcgaaa ctgccgatca ccgtggtacg caataaagcc
    43861 gatatcaccg gcgaaacgct gggaatgagt gaagtgaacg gtcacgcgtt aattcgtctc
    43921 tcggcaagga ctggtgaagg cgtggacgtg ctgcgtaacc atctcaaaca gagcatgggc
    43981 tttgacacca atatggaagg cggcttcctg gcgcgccgtc gccacttaca ggcgctggaa
    44041 caggcagcgg agcatttgca gcaaggtaaa gcgcaactgt tgggagcctg ggcaggtgaa
    44101 ctgctggcgg aagagttgcg cctggcacag cagaacttaa gcgaaatcac cggggaattt
    44161 acttcagacg acctgctggg gcggattttc tccagcttct gtattggtaa gtaaccgcgc
    44221 ttacgaagcc gtattctgac tgtcagatgc ggcttcgctt cattgttagc actcctgtta
    44281 tgcctcaacc cttttttaaa cattaaaatt cttacgtaat ttataatctt taaaaaaagc
    44341 atttaatact gctccccgaa cgattgtgat tcgattcaca tttaaacaat ttcagaatag
    44401 acaaa
//
LOCUS       DAEBXN010000026        42827 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.026, whole genome shotgun sequence.
ACCESSION   DAEBXN010000026 DAEBXN010000000
VERSION     DAEBXN010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42827
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.026"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            1414..2568
                     /locus_tag="KJL99_001409"
     CDS             1414..2568
                     /locus_tag="KJL99_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBD4436190.1"
                     /translation="MPSPRIRKMSLSRALDKYLKTVSVHKKGHQQEFYRSNVIKRYPI
                     ALRNMDEITTVDIATYRDVRLAEINPRTGKAITGNTVRLELALLSSLFNIARVEWGTC
                     RTNPVELVRKPKVSSGRDRRLTSSEERRLSRYFREKNLMLYVIFHLALETAMRQGEIL
                     ALRWEHIDLRHGVAHLPETKNGHSRDVPLSRRARNFLQMMPVNLHGNVFDYTASGFKN
                     AWRIATQRLRIEDLHFHDLRHEAISRFFELGSLNVMEIAAISGHRSMNMLKRYTHLRA
                     WQLVSKLDARRRQTQKVAAWFVPYPAHITTIDEENGQKAHRIEIGDFDNLHVTATTKE
                     EAVHRASEVLLRTLAIAAQKGERVPSPGALPVNDPDYIMICPLNPGSTPL"
     gene            2719..3543
                     /locus_tag="KJL99_001410"
     CDS             2719..3543
                     /locus_tag="KJL99_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001238934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HBD4436191.1"
                     /translation="MRLNTTGIAARMMLSLDKKAIGEKLINGRQINPTPLQVRYPQGV
                     REVLGIMSEQLAISTADLTRILLEDALHNMFMPADNTTGNIISRIEYIMLSHDINAPL
                     LATLLSPWNIRSTVIQDPARLADYLSADALEHLAECFNLNPDWLNGHENYPIALSGEW
                     PDTADNFRMLINDSSNTEVIFWHSFPFAGNTKREYCGVILRQEKEINGSVIYPALSLS
                     PTLLNDEKRKWLTEYTTRQNTTMSLRRVTLRPGLAGNLITGQILPVSLFNTSLLPW"
     gene            3629..4831
                     /locus_tag="KJL99_001411"
     CDS             3629..4831
                     /locus_tag="KJL99_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HBD4436192.1"
                     /translation="MMKKNHITRTIIASAVLFSFNAAAATSYFEARNDAMGGTGVASS
                     HYGVAPLANPALLTKHNSNDDFSLLLPSVGAQVADPDDVSNKADDVKDDWDLFDSAVD
                     NQHGVQQAAANLKHRLQEFRNINADAQVGVSAVAAMANDTLPFALMVKSYGTVSVNGK
                     VNDADLDYLDKVANGTITDVDKNALTSRAFGRAAVITDVGISFAKELETAGQKWSLGV
                     TPKYQRVDLFNYNVTVRDYDKDDFDGDKYHNTKNGFNADIGAYTDLNDNWTVGLVAQN
                     IIPRSIDTKVVNGFKETFKVRPQATAGVSWHNDLFTTALDVDLTPASGFTSDSKRQFA
                     SVGAEFNAWKWAQLRAGYRQNMASNSGSAFTAGVGISPFDVVHIDVSGLVGTDHDYGA
                     MAQLQFTF"
     gene            4891..5475
                     /locus_tag="KJL99_001412"
     CDS             4891..5475
                     /locus_tag="KJL99_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBD4436193.1"
                     /translation="MMKPRSSYSKTAFILLFSVFLVAAVTKAKSSLPDITLEQAKEIN
                     ADNTVIFLFRHGERCDRSDMPCYSDKSGITITGTEKAQQEGIKFATIFSEYDIYSSNA
                     VRTIQTAKFFSGKEPVVMDSLSDCNNDLYKTLESIARESHKRNIVIMTHNHCLSFLAR
                     DRLGKKFKPAYLDALIMHYDGTRLILDGKYNKEA"
     gene            5869..6327
                     /gene="traH"
                     /locus_tag="KJL99_001413"
     CDS             5869..6327
                     /gene="traH"
                     /locus_tag="KJL99_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer lipoprotein TraH"
                     /protein_id="HBD4436194.1"
                     /translation="MNRIIPAGCLAALFLAGCNSPSQHTPVPRNYGAASDVAVTRHTQ
                     YTLWDKGVFSGQLPVSTGSHLLTANSDRLSFDWEGDAIELLNELARVRGMQFNYNGVR
                     LPLPVNLHVRDMTFSNTLRLIEAQTAWRATIHQYPGLLQVSFMQPENRKK"
     gene            6324..7142
                     /gene="traI"
                     /locus_tag="KJL99_001414"
     CDS             6324..7142
                     /gene="traI"
                     /locus_tag="KJL99_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer lipoprotein TraI"
                     /protein_id="HBD4436195.1"
                     /translation="MKALTSLILPVILLTGCSSSPTISPGGVNTPPPDINAWLKPRSE
                     KPDSLSETRWKMLTDAGKTLGFRGGKAQRSWELIQALNARESTLNALYDFRPLISPEG
                     WLPPVIDEAQDVAHITPDQIRTSSRVWTIIRPERFVSNPPGWRDWLLRGLSTTATPGT
                     EGSVVPEDSVQRKVWETALRQGWQEGRQNADLTLEANQKTLTRDYRGMMLYSLLWRQG
                     MITRPDVSDQMQTVTGDGKKLVTGDRVRRLKNHAEFNLQKSHWRPLISTEGGSR"
     gene            7139..8287
                     /gene="traJ"
                     /locus_tag="KJL99_001415"
     CDS             7139..8287
                     /gene="traJ"
                     /locus_tag="KJL99_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid transfer ATPase TraJ"
                     /protein_id="HBD4436196.1"
                     /translation="MNIPDEFGLFPFQRFTADELRHFFVWCAAHKVSDVDLTGGSPVS
                     VSRFGRRVRCSSATLPTTLMSSLIDELFGREVIPRVLAGNPVDRTIQINGDASGRYGL
                     KRGERIRLRSHLIQGTSGAEEKAISITMRVIPTEIPDILSMNIEPNLLEAMVCKSGLG
                     FVCGETGSGKSTLCAALYCYIKKNYPNAKIVTYEDPVEYILGNENDLLPPHQAEIGRD
                     VASFAAGLRSAVRRNPEIIGVGEIRDNETADAAVQAGNTGHYCLSTMHTKSPGETLAR
                     LLGLFPPVIRDSMAWAVLSLLQFILVQVLVRTNDGGRKAVREYIVINDELRDNLSGMP
                     HAEWGHHIDAIIRQEKRRIRDQILEMYIRNEVDRREAILFIPPGELRS"
     gene            8284..8574
                     /locus_tag="KJL99_001416"
     CDS             8284..8574
                     /locus_tag="KJL99_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBD4436197.1"
                     /translation="MKREGFWRYATPPVNIQGVPLPFTMIYLFICPFPSKTTFWICTG
                     IILFFVILDRSGWTVRTICTRIFSILRGAIASGRPWWYRHHTESPRDWTGLD"
     gene            8589..9140
                     /locus_tag="KJL99_001417"
     CDS             8589..9140
                     /locus_tag="KJL99_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HBD4436198.1"
                     /translation="MSGKKTIVTLLRVSLLACPLLFTTPSFAMIDTPSVKVGFSPEGS
                     ASALVLDTINSAESSIRMMAYSFTDPDVMHALAKAKKRGVDVRIVVDDKGNTNRASQE
                     AMKYINLLDIPLRTVDAFPIHHDKVIIVDGNTVETGSYNFSRAAARKNSENVVVLKNM
                     PDVAAQYLEHWQDRWNKGTDWRP"
     gene            9230..12997
                     /locus_tag="KJL99_001418"
     CDS             9230..12997
                     /locus_tag="KJL99_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBD4436199.1"
                     /translation="MPSVSRYRTWLAVPADEIEDLKKAHPPMNGHTPVIWDKEHKLWF
                     ARPGADLSRLDRWLPRPQDVSMNGSDPVTEFAQVLENAGLVLKELPVMDGKIHRVPTA
                     DDKKGQKSGAYRGFLDGRPAGWYRDYRSADNSPITWTFSGGEQTDPRARLHLKAHSMQ
                     RREDAERELKAQYNRQAAYARRYINKWPQATAHEYLTRKGIQAAPGVRVNNKNELVIP
                     FSNRNGAIRSYQRIPVTGGKDARILIDSEKTGNWFALGTPRNGQPVLFAEGYATAASL
                     HEATGLPVLMTVDGGNMIAVAENARQKWTQSPFIFCADNDHAIRVNKGIVSATKAAEL
                     TGGSVIFPAFTDAEKAQGLTDFNDLDASRGRAAFQHVINAQLEHIGVSTPNSNTPEMR
                     EALVIGNLVFTPVHTEEKTMTPTEYPETSPDTGHSHDQGPSLPSATQQEQPAASSSNI
                     ADETQSFTSHATENNGKDERHADNVQAQTESSVVQDSPAESPASPESTTASAPDEPAH
                     PAEPPEKVVSVATDKDWREFEAELSQPEKNESQQESGTSPEIPAPAAAPASPEDSPSS
                     ETMNESAEPSPSPDAVKEEMTVMTEPDDKQSTGISPDTPREEAVNHVTEDIPLQPEPF
                     LPESDGPEEYEDYSAYQELMNNENPEHLQENSDMPSQPDTGHVQADESELQATTATIE
                     PAVRDEPTPQQPVQSTSPSDNTSSFLDKARGFFTRKKTDSQAHENSDPTPSAETTTTP
                     PIPDSIVYAPERSDAPISLNMDEILKELDWEKRADRTVLYKLDGKPAFIDRVNRLEMV
                     NGASNDDRSVLAALAVATKFYGGVIELTGSDAFKQKAMQLIIEHNIDVRMKLPDQRAE
                     LEKLRKEMAVSKDAVVTHQPTPELNRNIPEQPAVPDPVQEKEATQSPASPAAPEASTV
                     STAPASSPAEPGKTADAAPGEEPPGKLRPGESVTAVLHNFGRAEYAPGKGESFFVELK
                     NRSGSKLYWGEQLESLVKNHQKGDVVTLTLQNREQFILPGEQKARFRNKWSMESVTNG
                     ISVSHDNPDKGQRIQAIPVETFMKVAAQISQGWPEEIKALRMPENVGSHLFIGEDRHP
                     VSAPQNANQVTEITSAAPDKLTPVLGSVDKDTRELNLLLVQSADEHLQGVVRLNGTLY
                     PALATPSADNSQLVINALTDKGLRFAGYGEAVNHDADSTNRPAPELMQFHLKTREEPL
                     FAAVYTPEKQPDALYRNLGFEQSWQQWSNSQKPEDRQEKTLHQDLSHSPGR"
     gene            13015..13362
                     /locus_tag="KJL99_001419"
     CDS             13015..13362
                     /locus_tag="KJL99_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001629514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBD4436200.1"
                     /translation="MNMHKSLVAVLGLILISSTASAATCRAGSAAQAGSNTGYERARA
                     AADAWSQRENDVSSSLQSCLSRIKKISINLPQFPSLDDILSQLENQVCDAVVDKVNEK
                     LPGNIDPWKDYNL"
     gene            13359..14051
                     /locus_tag="KJL99_001420"
     CDS             13359..14051
                     /locus_tag="KJL99_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotI/IcmL family type IV secretion protein"
                     /protein_id="HBD4436201.1"
                     /translation="MTENTELSSPSHPAPDPEILKSTIRAMKQSEQRANNVPALIKAL
                     LCTGTCLLISITGNAIQYWHSTNVEREYFATDNGRLVRLAPTSQPAWSQNDAMAFGSQ
                     ALATAFNLDFVHYRSQISSLSPRFSDEGFVGYVNALQASNILETIKKEKMNLTATTGA
                     GVLVRQGQMSDGVWFWTFQYPVRMRLVGQTTSKPEQSFVFEITIQRVDPRLKPSGMEI
                     RQMISRNAGPNS"
     gene            14062..15045
                     /gene="traN"
                     /locus_tag="KJL99_001421"
     CDS             14062..15045
                     /gene="traN"
                     /locus_tag="KJL99_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraN"
                     /protein_id="HBD4436202.1"
                     /translation="MQSRYLLSTLLLVCSAATSADNAGWQNARTPQTTNSASDHVQNA
                     SQNTGGVPATLVKGELPAPGQASPLVQDAARLDSELSADEIRSLRSLMADNERAINAP
                     ITSVVPRISSLTVNLSPGASLPLVRTAMNNLSVVTFTDINGSPWPQSDPPYNAAPKLF
                     DVQYNENMVTITPLRPWASGNISVYLKGLSVPVILNVTSGETDTPSSSQEMDSRLDLR
                     IPRQGPTSPVVSIPTDKIALHDATLQAFLDGIPPRDPSVKRLKFTGNVPDTTIWQHGD
                     DLLVRSRAMLRDEFEQTLSSADGTHLWKLPVTPLLTFSVNGQSVHVTPELE"
     gene            15048..16337
                     /gene="traO"
                     /locus_tag="KJL99_001422"
     CDS             15048..16337
                     /gene="traO"
                     /locus_tag="KJL99_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraO"
                     /protein_id="HBD4436203.1"
                     /translation="MSAEQDAGKSGKKLAALLGLGSIILFGGGYIAFSKLSGSNSDMQ
                     SAVNINSAASGGTRSVTETPHYRELLRADNERGAAAAARNNQTFIASLPQGLDIPDSQ
                     EKQQQPAAKPENYAHRQASGTPQEDRAASEKRMERLQKLIVRIKDQHPAGSTPTIATA
                     MWNKSPAETTGQNGTQQFALQNASLSTPVAEKGIQLIPALTRIPAYIDTAVDSDNQSS
                     KVIATIPAGPWAGATLFSPGVKLVGNGVEIHFDRMSWNGMDLKVNAYAQREDNLMSSV
                     ASNVNTRWFKHIILPSVLGGVGSIGTLYKDANTQVIQGNYGTVTGRVGMPSGEAVAGV
                     IAGGMAERGSQILTRQAESEPYKQVEVYQHEVVSILFVDPVMTNDARSSSLSSGISPS
                     VNRTSQTEQRSQARMQTAMEQRKAVMQRRYDEQPETP"
     gene            16337..17041
                     /gene="traP"
                     /locus_tag="KJL99_001423"
     CDS             16337..17041
                     /gene="traP"
                     /locus_tag="KJL99_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraP"
                     /protein_id="HBD4436204.1"
                     /translation="MKPETEIDETGSFGEPEEKPAPFWKRSVWGISVATWGLCAVVLL
                     AAIWYLFLRAPSETGMPPFNDADAGVQTWQTTQESSPSVQSTETMTVRTGEDMSQLAR
                     DVKTELDNRDEKIQATLNMLHDSINKLGEAIKKDEEYAQETRRQLDDIRSHLNGMMTQ
                     KSVTESSSTPHPAKKKTSSVLNGMKIMSMETGMVWIRWQGSTWAVREGQTLGNVVIQR
                     IDPTTRTIITSAGTLR"
     gene            17041..17568
                     /gene="traQ"
                     /locus_tag="KJL99_001424"
     CDS             17041..17568
                     /gene="traQ"
                     /locus_tag="KJL99_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraQ"
                     /protein_id="HBD4436205.1"
                     /translation="MNMDALTAIENFASSIFSAGMDFLFTWGEFIGVISMITLFARAR
                     SAGPVKMSPGKFIAGMLTSCMLVSLPAMINAGGVQMGFRADSFGPIAYVQPQTFGAAA
                     GAANAVLSLAKLAGVGFVMNGISIWRKAGLDGHTALSASESVSKGNVKFIAGVLLVFI
                     DRVLNALLASIGIVF"
     gene            17619..18023
                     /gene="traR"
                     /locus_tag="KJL99_001425"
     CDS             17619..18023
                     /gene="traR"
                     /locus_tag="KJL99_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraR"
                     /protein_id="HBD4436206.1"
                     /translation="MSRIRVVAARLYAAFIHAQLFVMSCRSKLTKYLLLIPMMLLPKA
                     VLADGDLADMVRNVGEGAKTAQSSSITIAQFIGVILFLGGLIGLKKVGKQGGMGLAGC
                     IVSIVIGAVLVAGPEMMSRSQKQLGISSISIG"
     gene            18087..18275
                     /gene="traS"
                     /locus_tag="KJL99_001426"
     CDS             18087..18275
                     /gene="traS"
                     /locus_tag="KJL99_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative conjugal transfer protein TraS"
                     /protein_id="HBD4436207.1"
                     /translation="MSAWSPEHITLRRTENGNVIAHDSRDDQEFIFAECCSDEIANII
                     LEAVKRYTSTEPAHATQH"
     gene            18259..19059
                     /gene="traT"
                     /locus_tag="KJL99_001427"
     CDS             18259..19059
                     /gene="traT"
                     /locus_tag="KJL99_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraT"
                     /protein_id="HBD4436208.1"
                     /translation="MQHSIKDLWLYPFPEIDVVHTQEPLLPEPELTTPGRCICCRQNV
                     RHRFRLDDSWPLRQLTDTISDTRVRLNKATEHLDKLKKRGEPVATGEKEKYNTAVKAA
                     ERALEQARLSARRLSLRHVQKAEITSTESLSEKEQELFHEDGPPYSLCAFCHAWHSLN
                     GYAAAQGVMVWLPDLHPSTVVALNRRSLQEVFSNDKFRVRRGREALSALMQNRLAVED
                     KFRSFRPADFADVFRRYPPSGRSPLREKMNGIALILTPDSFIKKEYVD"
     gene            19149..22193
                     /gene="traU"
                     /locus_tag="KJL99_001428"
     CDS             19149..22193
                     /gene="traU"
                     /locus_tag="KJL99_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraU"
                     /protein_id="HBD4436209.1"
                     /translation="MNINRLIFTIEDCLNTLSRFSVASSFVEYCDLRTVIGLDRQDRE
                     RRPWLNSPYIAATKRGEYLSVFEVSGAFREMDEASDQTGLGSLESLITSMSDSLNTAY
                     KNSGHKISCVFERDPDMGKEEIEDMVAPQKRSLANTGIQLQDVVDEKVTTLSPWLVRE
                     RCWLAIWSGPDLISNSDRTAHDELVRRLAERVPKARFAQSPWQWTLSALKIRHEAFLD
                     NVEQALRHSSDGLILRLLDIHEVGREIRRQTERHSTPRNWQPHLPEDAQPAGYRWTDD
                     ESVLHAPSLHLQLFNTQVTTQGNLVQAGGLWHGMVSITLPPQNLQTFNELVRAVPRAV
                     PWRIRMDLMPGGMKALNLKKTLLTYSSFISAVRPMYESVMTLAATDEKEPVCIMTIMA
                     STWGKTREICTRNQAILKSAIEGWGVCGTTTTFGDPRRAWVNTILAASGGSGPVPLYP
                     PLSHAISLFPLNRAGSVWRGKGNLMLHTEDGSAFEVGLASSQQNKHTELAPGDPGLGK
                     SVLINTLSEIQISSAQKNLPFIAYIDKGYSAQGLVQLIRDSLPPERKDEAVGIILSND
                     PEYTRNLFDVMYGAKKPITPEKNFMSSVLCALCVDTGTGQPCNPGDTRQIINQLIELA
                     FKEYGENNPRLYRASTEDLVDSALQDSGLYEKHDAAWWARSTWFEVRDMLHNAGYIMA
                     AQRAHYQAMPQLPEVSSMLGHTSLRDVFGTVQRDGSNELLLDYIRRALEQGHNDYPMI
                     SGYTRFMINPETRVIAVDLNNVAGDKTPAGRLKTGIMYLLAGQIAGGDFTLPQYRDEV
                     LKQLPREYHEIALKRINQLDQEVKTKVYDELHNARGIDFIWENLDTQEREQRKFAIRT
                     VLSTQYLRDYPESVLKSANTLWLLRYKPEDIPVLRDNFNVPEFMLKRFLKMPEGPAPD
                     GSGVPVLGVFRVKSGTLARILKFTVGPLELWALNSSPKDSALRKTLTNKLGSVRARKI
                     LAENFPRGSATSLIEHRAGQHNSDNVIEDLASELIRKQGYNL"
     gene            22193..22807
                     /gene="traV"
                     /locus_tag="KJL99_001429"
     CDS             22193..22807
                     /gene="traV"
                     /locus_tag="KJL99_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000337395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraV"
                     /protein_id="HBD4436210.1"
                     /translation="MCTIHITPRCRRPARGQVFLKKLEVKGDSFSFTLPSRLFRLHPA
                     PNPRVIYASDARTQGKVTGMRLLLPSSTDELITTAIWQVSVPDEAPFDVQHVIQWILP
                     SPLASLMSDDTWLWPSVPGTPALSAGDWPVMDTDPSTLRRVRRRSFSIIDNRNRKGYI
                     TRRYQCFPLAPLPEDRKYDSLPDLVLHPEQETIKCKEKHYSQPL"
     gene            22774..23976
                     /gene="traW"
                     /locus_tag="KJL99_001430"
     CDS             22774..23976
                     /gene="traW"
                     /locus_tag="KJL99_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraW"
                     /protein_id="HBD4436211.1"
                     /translation="MQRKTLLAALIATLSGTACQAHAYSVTVVASRPVEEQVIPRMEA
                     IKDVLGNILSTQTATGTAINQNSEKLASVIAQNGQATRQQMIFSNETQRLEEARKSFT
                     VPDSICSESASGIATESKSASASAASKLSKGGGVSNRSIRDRLASAANSPVREAYDGA
                     AIHASYCTEAEYARFGGTAVCPSVGEIPGGDSQVRSIYHGAGTADTPAALTWDQKQID
                     AATAYMKNTSRPSAGRALGKGEVNTQSGRTYVGLQNEYNGIIDSASNPQLTLIADSTP
                     NESTRKALAETLQSDSAAAYFDQVASPEAKARGYMSTREFEAFEAGRRYANTAYLVDL
                     QEMQGDNLLRELVRITAQMNWQLNDLKEQIRQGNVISGQQLALTARQYYEKQLGSLEK
                     TINQANAR"
     gene            24005..24589
                     /gene="traX"
                     /locus_tag="KJL99_001431"
     CDS             24005..24589
                     /gene="traX"
                     /locus_tag="KJL99_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraX"
                     /protein_id="HBD4436212.1"
                     /translation="MNKLPENSDQTKNVKKSPSITRIATRGIINIFVPLMETRLITNS
                     VQHMLEQQKRRLDRFRQVNNKKAQLHLTWEEALQASHMSVDDLDRRFRRRRTVWRFCC
                     WSLLAIALFLSGMLFAASSLPLTTLVRAISTLVLILSGVALCASRALIVTYRLWQLHE
                     RKVSEPEQGTFRDFLNDRNGWRNATLIAVTSKQY"
     gene            24686..26854
                     /locus_tag="KJL99_001432"
     CDS             24686..26854
                     /locus_tag="KJL99_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotA/TraY family protein"
                     /protein_id="HBD4436213.1"
                     /translation="MKILLRALCAGLAISSLPAMASVTYQDIVSAATNPDDLSRQALV
                     TIFGDVVTNPLSTSAPTLIGSMFGAFNSIIAVLAVVWFMFIGIRHVVRSGHQGQVFST
                     GRDIVGTLSVVAGFLMIVPTGNGWSIAQLIMLWGASIMGVGSANVMVQLAADNIANGY
                     SMTVQPVQASTRTAARGIFEMELCKYAVNAGLNDFNQTVKSSTSLMTESAKTASGNYT
                     VTVSNGSGICGSASLSVEGNGTTDQSTIGKFFNPFSKNEYSGVISAQRAAMDNMISDM
                     DNAASEFVTTFLEKRNSGNGTLPDIETRIQRAADEYERAVQKSLPIDNGEQSRKEALK
                     SYLTTYGWVTLGAWYQTFATANQRLAELADRAPAVTSMSSLGEVGDTDLFSAVMGAYK
                     TQLQNSTYTPTIGTITTQDESNAANSTDPQSVVMKSIAPTILKWTNQAATEWSGTGTT
                     SNQVNPLIKMKNIGDYTLGTTEILWTGYTTVRVLATMGDNSIFGKAVNLFSGLPKGFV
                     ALLDAAAPPIYFLLFLLFCAGFSLSIYLPFIPFIFWMTGIGNWIVSVLIGCTAGPLWA
                     ATHLGTSEDRGSRAAYGYIYLIDSMIRPPLMVFGFFFASVAIIAVGTILNSLFGAALV
                     NVQVKSLTGIFSLAGFLLIYARMCTTSVAAIFALQAYLPDHVINFLGGRDGVNTLGNL
                     TSSVKDIFAGSNRNIRHSPGVREDRLKNVTKDDNKDGIKG"
     gene            26928..27578
                     /gene="excA"
                     /locus_tag="KJL99_001433"
     CDS             26928..27578
                     /gene="excA"
                     /locus_tag="KJL99_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid IncI1-type surface exclusion protein
                     ExcA"
                     /protein_id="HBD4436214.1"
                     /translation="MQMVKTINRYPAWWTLYYVLRFIYFISIPVLSIFILFGVLSITS
                     SRYVTQEDYIYTCVCLFLLIAPAILMYSRASSRKDKIKKIVAEIKNTGFYSPDKEYEG
                     LSFTQGVYFGVDTKKGTMLYARAYPGNIMDIIGFDIDNFTRTVTDAKTLEIYTKYINI
                     PMVSIPSGCIHPKMMADTMHAMAERGYDYPVDFPRLIQEKRKEWEQIAGMPVAEVF"
     gene            complement(27650..27859)
                     /locus_tag="KJL99_001434"
     CDS             complement(27650..27859)
                     /locus_tag="KJL99_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HBD4436215.1"
                     /translation="MSMPPAIANTFLFEMMKSKSKDVTLAAIYALGEGRCQAENITRE
                     LHRLSQSDDMEIKIAAIKALGRIYR"
     gene            28251..28427
                     /locus_tag="KJL99_001435"
     CDS             28251..28427
                     /locus_tag="KJL99_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4436216.1"
                     /translation="MNLVDAFVKKVISGPYKEYGKWWIDVEYISWGVPGKTRLMFESK
                     EQALEVKEGYKFLT"
     gene            complement(28492..28788)
                     /locus_tag="KJL99_001436"
     CDS             complement(28492..28788)
                     /locus_tag="KJL99_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000115013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4436217.1"
                     /translation="MSALRHVLSGILFSGKNRLQVLAKTSGGAGFGALVGEELSITDT
                     IETVVTGSVRANNGVLSPVMEKRMINLLIIVLRAVVAVANALIAVLELIRELID"
     gene            29088..29339
                     /locus_tag="KJL99_001437"
     CDS             29088..29339
                     /locus_tag="KJL99_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4436218.1"
                     /translation="MSEKWSKEFDSCFQVGSIEEIADALEKRSGSEENALAMLAMTAF
                     TLMTRRGICEMQNVAPDGKGIRLELIGFRENETIPDTLH"
     gene            complement(29411..29563)
                     /locus_tag="KJL99_001438"
     CDS             complement(29411..29563)
                     /locus_tag="KJL99_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HBD4436219.1"
                     /translation="MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVLVATL
                     AYEVKR"
     gene            29855..31063
                     /gene="trbA"
                     /locus_tag="KJL99_001439"
     CDS             29855..31063
                     /gene="trbA"
                     /locus_tag="KJL99_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbA"
                     /protein_id="HBD4436220.1"
                     /translation="MSYNRQPVAEDPMQIWGAVGVLLILLLFVIWLFLPEVVYASCLI
                     LHTLWGLVDWGPFHNYAAPRYNLLAMTGNNAANISYSQWVNVMEQTIGILWMYLLPVT
                     LWCLWEWYQHPGQSRFTRRPVDITRLPHIFASLSPAIAPVLADGDPEKLFHGGKRPER
                     RVALTPEAFVEQHTLITNMQLDVAAARRCFMAQLGKPLTSWKDMAPHEKALFAIFGLQ
                     YFLDDRKAALKLMDTLNLSCRIKSKRDSGKFCTPVYSLAKSAFQRVIKSNGAQQWLKQ
                     HRYVRSGLVWLYAHDLRLTPPNWIWLKGVDRTLFYALHRANTTKGFIEGAGVVAVARA
                     EAEAMRFGLPCPEPCVDEAVEGLRRDMLSLGLIWDEPQPDRDRKRRILTNWSLTDDIL
                     PRTPATDNEF"
     gene            31082..32152
                     /gene="trbB"
                     /locus_tag="KJL99_001440"
     CDS             31082..32152
                     /gene="trbB"
                     /locus_tag="KJL99_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbB"
                     /protein_id="HBD4436221.1"
                     /translation="MTIEYFATRIAKHISVQAIWPNGRTEIIAVLPRDALSGLVTRNN
                     QLIHIAFDAMGTRNRETVLIDKQEHNADQILTAIDSCLRLEYLMERRFSRTPLFRGII
                     ASVVLFVMATIGFSLFRYVDRVFWDDTTPEAVQTAGEPRLLPPHLNHTVPLNEGIQLP
                     VPPKKDVQVPEKTITSKNPEAAAARHNLATVLKRNADRGMFTINLSSGHERTLFAFLD
                     PACPNCRLLEPALKRLASDFNVVIYPVSVIGGEESTDRVAPLLCEKDAQKRAAGWHRL
                     YSADNGMMTPSEETTPADETCLKAARAAIDVNNVAFRKFGFAGTPWVLSDTGWHLPTG
                     ILQETGTLNLFLKTTDSESGHE"
     gene            32145..34436
                     /locus_tag="KJL99_001441"
     CDS             32145..34436
                     /locus_tag="KJL99_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbC"
                     /protein_id="HBD4436222.1"
                     /translation="MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIW
                     PLALPACLLFTLITMLVFSLQRWRCPLRMPMTLECADPSQDRMIKRSLFSFWPTLFQY
                     EVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIMFFATTGGGKTETIFAWAINP
                     LCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEIILSGEKTR
                     PQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGK
                     TMSLQMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWT
                     EEPRKQHAYLSGQFSETFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTS
                     AHTTSALGRMFITQKSMILARDLGYRLEGTDSDALEVKKYKGRFPYLCFLDEVGAYYT
                     DRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLMQNTGTKFAGRIVSEGSTA
                     RTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELHPGEFFS
                     IFRGETVPSASFFIPDDEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTAQRRIPSPE
                     NVSAIIGVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESA
                     LWETAVNTLKTTTREERRIRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNH
                     PTGRPVNGFHHKKTNRPDWDGMY"
     gene            complement(34473..37172)
                     /gene="nikB"
                     /locus_tag="KJL99_001442"
     CDS             complement(34473..37172)
                     /gene="nikB"
                     /locus_tag="KJL99_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type relaxase NikB"
                     /protein_id="HBD4436223.1"
                     /translation="MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQ
                     PHRSRFSRLVDYATRLRNESFVALVDVMKDGCEWVNFYGVTCFHNCTSLETAAADMEY
                     IARQAHYAKDDTDPVFHYILSWQSHESPRPEQIYDSVRHTLKSLGLADHQYVSAVHTD
                     TDNLHVHVAVNRVHPETGYLNRLSWSQEKLSRACRELELKHGFAPDNGCWVHAPGNRI
                     VRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVNDWLSLHRRLAEDGLYLSQ
                     MDGKFLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNP
                     DFIAADVRPEKIAETESLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRV
                     QHGHLVICDGYDHNQTPVRADSVWSLLTLDNVNQLDGGWQPVPTDIFLQVTPTERFRG
                     RRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGYSISHCSPQIEEMITQGEF
                     TWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEPQAGPFV
                     SAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAW
                     REQWRKPDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLK
                     EDIRNERQKLIADGKWYPPSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDKSRTTTT
                     DRCVVLCEPGGTPVYGNTGDLEARLQKNGSVRFRDRRTGEFVCTDYGDRVVFRNHHDR
                     NALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAWHNVTGRTGHEDYRITR
                     PDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG"
     gene            complement(37183..37515)
                     /locus_tag="KJL99_001443"
     CDS             complement(37183..37515)
                     /locus_tag="KJL99_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="HBD4436224.1"
                     /translation="MSDKRQRSGSERRQKTILRAVRFSPDEDETICKNAEDSGLTVSA
                     YIRNAALNKRINSRTDDAFLKELMRLGRMQKHLFVQGKRTGDKEYAEVLVAITELTNT
                     LRKQLMEG"
     gene            37748..38083
                     /locus_tag="KJL99_001444"
     CDS             37748..38083
                     /locus_tag="KJL99_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HBD4436225.1"
                     /translation="MASKKFYSDDDIQLAKAALSELPDLTAQRKTLRDFLDAIRDDII
                     ILVRTKGYTLADVRDTLKNAGYEVGEKALRDIIREAESKKPSRRSSSKTAPKKTDSGR
                     KDGIDMNNN"
     gene            complement(38170..39018)
                     /locus_tag="KJL99_001445"
     CDS             complement(38170..39018)
                     /locus_tag="KJL99_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HBD4436226.1"
                     /translation="MNQTLPTADLNTAGTTDVIPSVAIDRIIAQRNEGIALFMQAMEC
                     LATARRILLDASGDIFLYGFEDCVTDSVRCMDKPEEAKKNITRLADRKIWDRLMTDTG
                     MYTFMSSCQRDEWNSQLMSDTCPEITLDNVLATFRYLNASKMQTFEQGLIDVYRKLSW
                     DYRTNNPCRLGKKIIIENLLYRWSNGRVTLDCSGREALDDLVRPFYLLEGRNVPDFRN
                     SIGAQYGEFLGNGDNVGKLLEGEYFTVRGYQKGTVHIVFKRSDLVEKLNDIIARHYPG
                     ALPPRV"
     gene            39367..39564
                     /locus_tag="KJL99_001446"
     CDS             39367..39564
                     /locus_tag="KJL99_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4436227.1"
                     /translation="MQGAPHPARLKKNDGTTMACGGHWHTTPAQVKTEKTARHLQPKK
                     VKFLHFFALVYRADLAIVMIK"
     gene            39598..39849
                     /locus_tag="KJL99_001447"
     CDS             39598..39849
                     /locus_tag="KJL99_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBD4436228.1"
                     /translation="MTGWELRIWRKSMLWSREKAAREFGVTQRTWHAWENAEQVDVTV
                     WRTTQALSVRDLLPHMQGMRKADIIRRLENELGETAEDV"
     gene            complement(39880..40086)
                     /locus_tag="KJL99_001448"
     CDS             complement(39880..40086)
                     /locus_tag="KJL99_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4436229.1"
                     /translation="MTIAERLIQKGFDEGFDEGFKEGFKKGALEVAREAACRLRDMGW
                     TPERIQEAAGLSGEELKKLFPDEQ"
     gene            complement(40148..41050)
                     /locus_tag="KJL99_001449"
     CDS             complement(40148..41050)
                     /locus_tag="KJL99_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBD4436230.1"
                     /translation="MSKKKNTTTPTPHDAAFRSFLANPDVARDFLELHLPAEYRQLCD
                     LSTLKLEPATFVEPDLHQYASDILWSVKITGGKDGYVYTLIEHQSTENLYMPFRMLRY
                     SVAAMQRHLEQHKTLPLVIPVLFYHGERSPYPYSMNWLDCFENPALAAKIYTKPFPLV
                     DITVVDDNEIMNHRRMAALTLLMKHIRHRDMMELLDKLPQVMVEISDEQVRVLIHYIV
                     NAGDSVSPEFMRALAERLPQHEDKLMTIAERLEQKGRQEGRMEGALEKALAIACQLQK
                     MGMTPEQIKQATGLSDDELKKIIH"
     gene            complement(41047..41436)
                     /locus_tag="KJL99_001450"
     CDS             complement(41047..41436)
                     /locus_tag="KJL99_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CbiX"
                     /protein_id="HBD4436231.1"
                     /translation="MKSCFDTDKAFHMSFDEVTFNADRIRSKLELLSEELMPRCGLVY
                     ELYQRRLSAAIDNFVATLPTEQHLLVLELAREEFDYLSAEEIAEEIRRDSERGYCCHG
                     FERNCCPLGCGDLGDDCCDAADLMEEP"
     gene            complement(41613..41879)
                     /locus_tag="KJL99_001451"
     CDS             complement(41613..41879)
                     /locus_tag="KJL99_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4436232.1"
                     /translation="MRPSIIFATAEYVKRLREECLRENKPLHRHTRFRRQELAQDEIN
                     PDVLAMSGHIARRCSEQKRVRIPAMKVSEWGHLLRALEIERVCH"
     gene            complement(41973..42407)
                     /locus_tag="KJL99_001452"
     CDS             complement(41973..42407)
                     /locus_tag="KJL99_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="post-segregation killing protein PndC"
                     /protein_id="HBD4436233.1"
                     /translation="MTVSSTISVFCRDGVFRTVYCHLHGEPTWNGRILHTHYATGQQA
                     EALVEHGDIRCLGPRCDKPSGHTLQNPVDGVTAYYGRDSGFRMDSEAREYRSFMEAIA
                     TESTEEVRFHYVFIDGYWKVMYRTPEGWKMKALALALRRCPK"
     gene            complement(42651..>42827)
                     /locus_tag="KJL99_001453"
     CDS             complement(42651..>42827)
                     /locus_tag="KJL99_001453"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4436234.1"
                     /translation="LKNQACSDRSGRAAPVAPETALPAHLTVCKATAPDGAVNPSAAT
                     PAACFPLWTFRGLE"
BASE COUNT        11358 a        11000 c        10363 g        10106 t
ORIGIN      
        1 ggcctacgtg ccaatccggt acctggaaat catcatcagc gtcgatatgg acgaatataa
       61 aaacatttac gttatatccc aaaaacacac aggtcttagg gaggtttaaa ctgtgtatta
      121 acacctatcg tattgacggg cgtgaaatgg cagaaacaga agtcgttcct attgacatgc
      181 ctgactctaa cggcgagatg acttggcaag ccaaaaacta cacacagtat tcgtcctact
      241 ttatgaagat tacttgcttg aaatagctta ttggcaaatg gcatatacag ttattgaacc
      301 attctgatct tcggttgtat cgcacgcagc cgaccatgag ttattggctg atgggaatga
      361 cctggtaagc catatgtaac cagttcgtga ggtgcatatt ccgccgccac ctgtaacacg
      421 ttttccagtt ggacaggtag ctgttgctcc aggaaagcgc caacctgtgg cctgtgctgt
      481 agcaatttga agctcaccag atcctacctt tctccaggta ccggattggc actggcctac
      541 gtgccaatcc ggtacttggc gtaaaagcaa ttctggtagt acggtgataa ctggtcgtat
      601 agcaaatggt cagcaaatac cgctgccaac aggattttct gccagtcagt gctcatggag
      661 tgtgagcaat gcagaaaacc ctcaaggttg gaagccgaat tactttgcag gatcagttgc
      721 aacttatgat gcaaatcgaa ttgttaaatg tggtttttat gatgaataca atttccataa
      781 agggacattt agagctgatt taaccggaaa atgtagttat gtcgtcgcgt gtcagaactg
      841 agattaaatt gctggcgctg ctggcctacg tgccaatccg gtacctgggg cgctcctaaa
      901 attcaattca caacgcagac ctacaacata gctaaaaaca ctcgtaattt aaggctcggg
      961 gtccatgcat attgctcatg gacttacctg aatggctcac cctttggtgg ttttcaacag
     1021 gtatattccg accaaaacaa cgtttggtat gtgagtaatt atgcttgggg aaattatgag
     1081 tctggtggga ccatatcagt cacatgcctc aatcttcctg gtgctggagc ttaattaaag
     1141 gggcaacagt aggcatcgac atgttcctca tcaacaccat caccaatacc gtacatgcgt
     1201 gttccagtca taatgtatcc agtcggacat tgaatcggct tgtaatagat cgaaccacca
     1261 ctcttaccgc cgataaagtg attcattgct acggatgatt tgtaccaatt acatgctgag
     1321 taattaattt tattaccccc tgaccaccga ccggattggc acgcttgcag agaatgattg
     1381 ttggcattat tttttgcctc ttttgaggtt agcatgccgt ctccacgcat ccgtaaaatg
     1441 tccctgtcac gcgcactgga taagtacctg aaaacagttt ctgttcacaa gaaagggcat
     1501 caacaggagt tttaccggag caatgttatc aagcgatatc ccattgctct tcggaatatg
     1561 gacgaaataa caaccgttga tattgctaca tacagagacg ttcgtttagc agaaataaac
     1621 ccccgaacgg gtaaagccat tacaggtaat actgtacgtc ttgaactcgc ccttctgtca
     1681 tctctgttca atattgctcg tgttgaatgg ggaacctgtc gtactaaccc ggttgaactg
     1741 gttcgcaagc cgaaagtatc ctccggacga gatcgccggc taacgtcttc agaagaacgt
     1801 cgcctttctc gctatttccg cgaaaaaaat ctgatgttgt atgtcatttt ccatcttgcc
     1861 cttgaaacag ccatgcggca gggcgaaata ctggccttac gttgggagca cattgatttg
     1921 cgccacggtg tggctcattt acctgaaacc aaaaacggtc actcacggga tgttcctctg
     1981 tccagacgtg cccgtaactt tcttcaaatg atgcccgtta atctccacgg caatgttttt
     2041 gattacaccg catccggctt taaaaatgcc tggagaatag ccacacaacg acttcgcatc
     2101 gaggacctgc attttcacga tctacggcat gaagcaataa gccgcttctt cgaactgggt
     2161 agcctgaatg taatggagat tgctgcaata tcaggacatc gttccatgaa tatgctgaaa
     2221 cggtatactc atcttcgtgc atggcaactg gtcagtaagc ttgatgcccg ccggcggcag
     2281 acacaaaaag tggcagcatg gtttgtgccg tatcctgccc atatcacgac tatcgatgaa
     2341 gaaaatgggc agaaagcgca tcgtattgag atcggtgatt ttgataacct tcacgtcact
     2401 gccacaacaa aagaggaagc agttcaccgc gccagtgagg ttttgttgcg tacactggcc
     2461 attgcagcac agaaaggcga acgtgtccca tctcccggag cgttacctgt taacgaccct
     2521 gactacatta tgatttgccc tctgaacccg ggcagcacac cgctgtaaac atcattcaaa
     2581 acacatacag aaaggcgagt tacaacaact cgccaaacac attcgatctg catgatcgaa
     2641 attattcgtt tcaccagaaa aaacaccgaa gatacaataa ccacatgagc tgaaaagact
     2701 ttttcagtcg gaggaacaat gagactgaat acgaccggta ttgcggcccg aatgatgctc
     2761 tcgctggaca aaaaagcaat cggcgaaaaa ctcatcaacg gtcgtcagat aaacccgaca
     2821 ccactgcagg tccgttaccc acaaggggta agagaagttc tggggataat gagcgaacaa
     2881 ttagccatat ccaccgctga tttaacccga atcctgcttg aggatgctct tcataatatg
     2941 tttatgcctg cggataacac cacaggaaac ataataagca gaattgaata catcatgctg
     3001 tcgcatgata taaatgcgcc attactggca acattgctgt cgccatggaa tatccgctcc
     3061 acggttatac aggacccggc cagacttgca gactatcttt cagctgacgc tctcgaacat
     3121 cttgctgaat gttttaatct caatccggac tggctgaatg gccatgaaaa ctatccgata
     3181 gcgttgtccg gtgaatggcc agatactgct gataatttca gaatgcttat taatgatagc
     3241 agcaacactg aagttatttt ctggcatagt ttccccttcg caggaaacac gaaaagagaa
     3301 tattgcgggg taatattgag acaggaaaaa gaaataaatg gctctgtaat atatccggca
     3361 ctatcattat caccaacact tctgaacgat gaaaaaagaa aatggttaac tgaatataca
     3421 accaggcaga atacaacaat gtctttgcga cgcgtcacgt tgagacctgg ccttgccggg
     3481 aaccttatta cagggcaaat actacctgtt agcttattta atacgtcttt attaccctgg
     3541 taaataccag atagtaccaa agtaaaaaat tacggcacat ccgtgtcata gcgttgtttt
     3601 gtctaaattc tgataaacag gaaattaaat gatgaagaaa aatcatatta cccgcaccat
     3661 cattgcatct gctgtattat tttcctttaa cgctgcagca gcgacgtcat attttgaagc
     3721 gcgtaacgat gcaatgggtg gcaccggggt agcatcttcc cattatggtg tggcacctct
     3781 ggcaaacccg gctttactga caaagcacaa cagcaatgat gatttcagcc ttcttctgcc
     3841 gtctgttggg gcacaagttg ctgatcctga tgatgtatca aataaagctg atgatgttaa
     3901 agatgactgg gatttgtttg acagtgctgt agataaccag catggcgtac aacaggcagc
     3961 ggcaaatctt aaacatcgtc tgcaggaatt ccgcaatatc aatgcagatg ctcaggtagg
     4021 tgtatcagct gtagcagcca tggcaaatga cactctgccg tttgccctta tggtcaagtc
     4081 ttacggaact gtcagcgtaa acggaaaagt aaacgatgca gacctggatt atctggacaa
     4141 agttgccaat ggaacaatta ccgacgtcga taagaatgcc ctgacctcac gcgcattcgg
     4201 gcgtgcagct gtaattaccg acgtcggaat ttcatttgcc aaagaactgg aaacggccgg
     4261 acaaaaatgg tctcttggcg tcacaccaaa atatcagcgt gtggacctgt ttaactacaa
     4321 cgtaacggtt cgtgattacg acaaagacga ttttgacggt gacaagtacc acaacacgaa
     4381 aaacggattc aacgccgata ttggtgctta tacagacctg aatgacaact ggactgtcgg
     4441 tcttgttgcc cagaacatca ttccacgcag tattgatact aaagtcgtca atggctttaa
     4501 agaaaccttc aaagttcgtc cacaggcaac cgccggtgta tcctggcata atgacctgtt
     4561 taccactgct cttgacgttg atctgactcc tgccagtggc ttcacttctg acagcaaacg
     4621 tcagtttgca agtgttggtg ctgaatttaa cgcatggaaa tgggcgcaat tgcgtgccgg
     4681 ttatcgtcag aacatggcat caaactccgg aagcgccttc accgctggcg ttggtatctc
     4741 tccatttgac gtcgtacata tcgatgtttc cggcctggta ggaactgacc acgattacgg
     4801 tgcaatggca caacttcagt tcactttctg attacagtcc ccgcccccgg cactaaaccc
     4861 gggggtattt ttatattcag tttaaataac atgatgaaac cacgttcttc ttattcaaag
     4921 acagcattca ttcttttatt ttctgttttc ctggtagctg cagtgacaaa agcaaaaagc
     4981 tcgcttcccg atatcaccct ggaacaggca aaagaaatta atgctgataa caccgttatt
     5041 ttccttttcc gccatggaga acgttgcgac cgttcagata tgccctgcta ttcggataag
     5101 agcggaatca ctattaccgg tacagagaaa gcacagcagg aaggtattaa gttcgcaaca
     5161 atcttcagtg aatatgatat ttactccagc aatgcggtaa gaaccataca aactgcaaaa
     5221 ttcttctccg gtaaagaacc cgtcgttatg gacagtttat cagactgcaa taatgatctt
     5281 tataagacgc ttgaatcaat cgcaagagaa tctcataaaa ggaacatagt aataatgaca
     5341 cataatcatt gtttatcatt tctggccaga gataggctcg gcaagaaatt caaaccggcg
     5401 taccttgatg ccctgatcat gcattacgat ggtacccggc tcatacttga cggaaaatac
     5461 aacaaagagg catagagata tttgaattta cataaattct gatatactca gtaagataca
     5521 agagtgtacg ggcacatgtg cctgtctgaa ttgcaggtta tccggacgag ggccgggaac
     5581 tgtttcaggt acaagcgccg gtacgttcga ttcataaatc gtcgagtcat ccatcatgga
     5641 tcgattcggc gcggagcgtt aaagattacg ctcacattca tattatgtct gacgaccaat
     5701 caaccagcca gtatcaggaa taatcacctt cagtaaacta tgaggctatt tacctggccg
     5761 cactggcatt tatttccggt aacgcaagta tagccggaaa caaacatcat cctgtctgta
     5821 ccggagcacc ggtatatccc attctgattt aactgctcag gaattattat gaaccgtatt
     5881 atacctgcgg ggtgtcttgc cgcccttttt ctggctggct gcaactcccc ttctcagcat
     5941 actcctgttc cgcgtaacta cggtgctgcc tctgatgtgg cggttacccg gcacactcag
     6001 tacacattat gggacaaagg tgtgttttcc ggccaacttc cggtttcaac ggggtctcat
     6061 cttctgacag ctaacagcga tcgcctttcc tttgactggg aaggtgatgc gattgaatta
     6121 ctgaacgaac tggccagagt cagggggatg cagtttaact acaatggtgt ccgcctgccg
     6181 ttaccagtca accttcacgt ccgggatatg acgttttcca atacgttgag acttattgaa
     6241 gcccagacag cctggagagc aacaatccat caatatccgg gcttgttaca ggtcagcttc
     6301 atgcagccgg agaaccggaa aaaatgaaag cactcacctc tctcattttg ccagtaatac
     6361 tgcttactgg ttgttcttct tcccctacca tctcgccagg tggtgtcaat acgccaccac
     6421 ctgacattaa tgcatggctt aaaccacgaa gtgaaaagcc ggatagtctg tcagaaacac
     6481 gctggaaaat gctgaccgac gcaggtaaaa cgcttggatt tcgtggtggc aaggctcagc
     6541 gctcatggga gctgattcag gcactcaacg cccgggaaag cacacttaat gccttatacg
     6601 atttccggcc actgattagc ccggaaggct ggctaccacc ggttattgat gaagcacagg
     6661 acgtagctca tatcacgcca gatcaaatcc ggacctcttc ccgggtctgg accatcattc
     6721 gccctgaacg ctttgtcagc aatccaccgg gctggcgtga ctggcttctc cgggggcttt
     6781 caacaacagc cactccgggt acagaaggca gcgtcgttcc agaagacagc gtgcaacgaa
     6841 aagtgtggga aaccgcatta cggcagggat ggcaggaagg aagacaaaac gccgacctga
     6901 cgcttgaagc caaccagaaa accctcaccc gtgattaccg gggcatgatg ctgtactcac
     6961 ttctctggcg tcagggcatg atcacccgtc cggatgtttc tgatcaaatg caaaccgtta
     7021 caggtgacgg gaaaaaactg gtcactggag atcgtgttcg tcgccttaaa aatcatgcgg
     7081 aattcaatct tcagaaatct cactggcgtc cgcttataag cactgaagga ggctcccgat
     7141 gaatattcct gatgagtttg gtctttttcc gtttcagcgt ttcacagcag atgaactacg
     7201 gcatttcttt gtctggtgtg ccgcacataa agtcagtgat gtggatctga ccggtgggtc
     7261 accagtttct gtcagtcgct ttggccgacg ggtgcgttgt tccagtgcaa cgttaccaac
     7321 cacactcatg tcctccctga ttgatgaact gttcggtcgg gaagtcattc cccgtgttct
     7381 ggcaggcaat ccggttgaca ggacgataca gattaacggg gatgcatccg gtcgctatgg
     7441 tcttaaacgc ggagaacgca tccggttacg ctctcacctc attcagggca catctggtgc
     7501 ggaggagaaa gcaatatcaa taaccatgcg tgtcatcccg acagaaatac cggacatcct
     7561 ctccatgaac attgaaccaa acctgcttga agcaatggtc tgcaaaagcg gtctgggatt
     7621 tgtatgtggt gaaacagggt caggaaaatc aacgctgtgt gcagcactgt actgttacat
     7681 taagaagaat tatcccaacg ccaaaatcgt gacatatgaa gacccggtgg aatatatcct
     7741 gggaaatgaa aacgatctgc tcccaccgca tcaggcagaa atcgggcgtg atgtggccag
     7801 ttttgctgcc ggactgcgtt ctgctgtacg acgtaatccc gaaattatcg gtgtcggtga
     7861 aatcagggat aacgaaaccg ccgacgcagc ggtacaggca ggcaacaccg ggcattactg
     7921 cctgagcacc atgcatacga aatctcctgg tgaaacgctg gcacgtcttc ttgggctgtt
     7981 tccccctgtc atcagggatt cgatggcctg ggctgtcctg tcactgttgc agttcattct
     8041 ggttcaggtt ctggtcagaa caaatgacgg tggtcgtaaa gccgtccggg aatatatcgt
     8101 cattaacgat gagcttcgcg acaatctttc cggaatgcca catgcagagt gggggcatca
     8161 cattgatgcc ataatccggc aggagaagcg ccgtatccgg gaccagattc tggaaatgta
     8221 tatccggaat gaagttgacc gaagagaagc gattcttttt atcccgccag gggagttacg
     8281 gtcatgaaaa gagaaggttt ctggcgatat gcaacaccgc cagtcaacat ccagggcgta
     8341 cctctgccgt tcaccatgat ttatctgttt atatgcccct ttccctccaa aacaaccttc
     8401 tggatttgca ccggcatcat tttatttttc gttattctcg accgttcagg ctggactgtc
     8461 aggacgatct gtactcgcat tttcagcatt ctgagagggg ccatcgcctc cggtcgtcca
     8521 tggtggtatc gccaccatac ggaatcacca cgagactgga ctggcctcga ttaaattctg
     8581 gagtctttat gtcaggtaaa aaaactatcg ttactctgct ccgggtatcg ctactggcat
     8641 gcccgttact gttcacgaca ccatcgtttg caatgatcga cacgccatcc gtgaaggtcg
     8701 gcttttctcc tgaaggaagc gcatccgctc tggtgcttga cacaatcaac agtgcggaaa
     8761 gctccatcag gatgatggca tattccttca ccgacccgga tgtaatgcat gcgctggcaa
     8821 aagcgaaaaa acgcggagtg gacgtccgta ttgttgttga tgacaagggg aatacgaacc
     8881 gggcaagtca ggaagcgatg aaatatatca acctgctgga cattcctctc cggactgtag
     8941 atgccttccc catccatcac gacaaagtca tcattgttga cggaaacacg gttgaaactg
     9001 gctcctataa cttctctcgt gctgctgcac gcaagaattc agagaatgtc gttgtgctca
     9061 agaatatgcc ggatgtagcc gcacaatatc ttgaacactg gcaggatcgc tggaataagg
     9121 gtacagactg gagaccctga caaacatttc tcccccgatt ttctatcccc ccagccaccc
     9181 ccggcttccg gcggtggttt ttttccgtct tttttcagga gaagcactca tgccgtctgt
     9241 atcccgctac cgcacctggc ttgccgtgcc tgcagatgaa atagaagatc tcaaaaaagc
     9301 acatcccccg atgaacgggc acacgcccgt catctgggac aaggagcaca aactctggtt
     9361 cgcccgcccg ggggcggacc tgtccaggct ggaccgctgg ctgccccggc cacaggacgt
     9421 ctccatgaac ggcagtgatc cggtcaccga gtttgcccag gttctggaga atgccggact
     9481 ggttctgaaa gaactgccgg tcatggacgg gaaaatccac cgtgtcccga ccgccgatga
     9541 caaaaaagga caaaaaagtg gcgcatatcg cggattcctg gacggcagac ctgccggctg
     9601 gtacagggat tatcgcagcg cggataattc gccgatcacc tggaccttct ccggcggaga
     9661 acagacagat ccccgcgcaa gactccacct gaaagcgcat tcaatgcaac gcagggagga
     9721 cgcggagcgg gaactgaaag cacagtacaa ccgacaggcc gcgtatgccc gccgctatat
     9781 caacaaatgg ccgcaggcca cagcacatga atacctgacc cggaaaggca ttcaggctgc
     9841 accgggggtg cgggtaaaca acaaaaatga actggttatt cccttcagta accgtaacgg
     9901 cgcgatacgc tcttaccagc gtattccggt caccgggggg aaagatgccc gcatcctgat
     9961 agattcggag aaaaccggta actggtttgc gctgggcaca ccgcgcaacg gtcagccggt
    10021 actgtttgcc gagggctatg ccactgcggc ctccctgcac gaagccacgg gccttccggt
    10081 actgatgacg gtggatggag gcaacatgat tgccgttgcc gaaaatgccc ggcaaaagtg
    10141 gacgcaaagc ccctttatct tctgtgccga caacgatcat gcaatccggg ttaacaaagg
    10201 tatcgtcagt gcaacaaaag ccgcagaact gacaggagga tcggtcatct ttcctgcgtt
    10261 caccgatgcc gaaaaagcgc aggggctgac cgatttcaat gacctggacg caagccgggg
    10321 acgggccgct tttcagcatg tcatcaacgc ccagttagaa cacatcgggg tttccacccc
    10381 caacagtaac accccggaaa tgcgcgaggc actggtgata ggcaacctcg tttttacacc
    10441 ggtccacact gaggaaaaaa ccatgactcc gacagaatat cctgaaacat cacccgatac
    10501 agggcacagc catgaccagg gaccttctct gccgtccgcc acgcagcagg aacaaccagc
    10561 tgcttccagc agtaacattg ctgatgaaac acagtcattt accagtcacg ccactgaaaa
    10621 taacggcaaa gatgagcggc acgctgataa cgttcaggcg caaacggaaa gctctgtagt
    10681 tcaggactcc cctgcagaat caccggcgtc cccggaaagt acaacggcgt ctgcccctga
    10741 tgaacccgct catccggcgg aacctccgga aaaagttgtc tcggtggcca cagataaaga
    10801 ctggcgtgaa tttgaagccg aactgtcaca accggaaaaa aacgagtcac aacaggaatc
    10861 cggcacttca cctgaaatac cggcccctgc tgccgccccc gcatcccctg aagacagtcc
    10921 ctcatctgaa accatgaatg aatcagccga gccatcacct tcccctgatg cagtcaaaga
    10981 ggaaatgacc gtgatgacgg agcctgatga caaacaaagc accggaatat ccccggatac
    11041 tccccgggaa gaggcggtca accatgtaac tgaagacatt ccccttcagc cagagccgtt
    11101 ccttcctgag tcagatggcc ccgaagaata cgaagactac agcgcatatc aggagctgat
    11161 gaacaatgaa aatcctgagc atctgcagga aaacagtgat atgccgtcgc aaccggacac
    11221 aggccacgtt caggcagatg agtcagaact tcaggccaca acagccacta tagaaccggc
    11281 tgtccgggat gaacccactc cgcagcaacc tgttcagagc acatcacctt ctgataatac
    11341 ttcatcgttt ctcgacaaag cgcgtggctt ttttacacgt aaaaaaacgg attcacaggc
    11401 gcatgagaac agtgatccca cgccctcagc agaaaccacg acgactccac cgatacctga
    11461 cagtattgtc tacgccccgg aacgttctga tgcaccgata tcgctcaaca tggacgaaat
    11521 tcttaaagag cttgactggg aaaaacgagc agacagaacc gtcctgtaca aactggatgg
    11581 caaacctgcc ttcattgacc gggtaaaccg gctggaaatg gtcaacggtg ccagcaatga
    11641 cgatcgcagc gttctggccg cacttgctgt ggccaccaaa ttttatgggg gcgtcataga
    11701 gctgacgggc agtgatgcct ttaagcaaaa agcaatgcag ttgattatcg agcacaacat
    11761 cgatgtccgg atgaaattgc cagatcaacg ggctgaacta gaaaaattac gcaaggaaat
    11821 ggctgtcagt aaggatgctg ttgtcaccca tcagccaaca ccggaactta accggaatat
    11881 ccctgaacaa ccggctgtcc ctgaccccgt tcaggaaaaa gaagccacac aatccccggc
    11941 gtcccctgct gcccctgaag catcaaccgt cagcaccgct cctgcatcat caccggctga
    12001 accaggcaaa acggcagatg cagcaccagg tgaagaacca ccaggaaaac ttcgcccggg
    12061 agagagcgtg actgccgtac tgcacaattt tggtcgtgct gaatatgcac ccggcaaagg
    12121 agaaagtttc tttgttgagc ttaaaaaccg cagtggcagc aaactctact ggggggaaca
    12181 actggaaagt ctggtcaaaa accatcagaa aggtgacgtg gtgacactga cacttcagaa
    12241 ccgcgaacaa ttcatcctcc cgggagagca aaaagcacgt ttccggaata agtggtccat
    12301 ggagtcagtt accaacggga tctcggtatc acatgataat ccggataaag gtcagcgtat
    12361 tcaggcgatt ccggtcgaaa cttttatgaa agtggctgcg caaatcagcc aggggtggcc
    12421 cgaagagata aaagcgttac gtatgccaga aaacgtcggc tcacatctgt ttatcggtga
    12481 agaccgacat ccggtctccg cgccacagaa cgcaaatcag gtaacagaaa taaccagcgc
    12541 agcccctgac aagctgacac ctgttctggg atcagtggat aaggatacgc gtgaactcaa
    12601 cctgctactt gtccagagtg ccgatgaaca tctgcagggt gtggtccggc tgaacggcac
    12661 attgtatccg gcactggcca ccccatcggc cgacaacagt cagctggtta tcaatgcgct
    12721 gaccgacaaa ggtttacgct ttgcaggtta tggtgaagcg gtaaaccacg atgctgacag
    12781 cacgaatcgc cccgcaccgg agcttatgca gtttcatctg aaaacccggg aagaaccgct
    12841 ttttgctgct gtctacacgc cggaaaaaca acctgatgcg ttgtaccgga acctgggctt
    12901 cgagcagtca tggcagcagt ggtccaacag ccaaaagccg gaggacaggc aggaaaaaac
    12961 tctgcatcag gatctctccc acagccccgg ccgatgagcc ggtaaggaag ccatatgaac
    13021 atgcataagt ctctggttgc cgtgctcggc cttatcctga tttcatccac ggcaagcgct
    13081 gcaacctgtc gtgcgggtag cgcggcacag gccggtagta atacaggata tgagcgagca
    13141 cgtgcagctg cggatgcatg gtcacaacgt gaaaacgatg tttcctcatc cctgcagtcc
    13201 tgtctgtcac gaatcaaaaa aatcagcata aacctccccc agttccccag ccttgacgac
    13261 attctgtcgc agctggaaaa ccaggtctgc gacgcggtcg tggacaaggt gaatgaaaag
    13321 ctgccgggga atattgatcc ctggaaggac tacaacctat gaccgaaaat accgagttat
    13381 cctcgccatc acatccggca cctgacccgg aaatactgaa gagcacaatc agggctatga
    13441 agcagtcaga acagcgggca aataatgtcc ctgcgctgat aaaagcgctt ttatgtacgg
    13501 gcacatgtct tcttatcagt attactggta acgccattca gtactggcac tcaacgaatg
    13561 tggagcgcga atatttcgct acagacaatg gtcgtctggt caggctggca ccaacaagtc
    13621 agccagcatg gagtcagaat gatgcaatgg cctttggcag ccaggcactg gcgaccgcat
    13681 tcaacctgga ttttgtgcac tatcgttcac agatctcctc cctgtctccg cgtttttctg
    13741 acgaaggttt tgtcggatat gtgaatgcat tacaggcatc taacattctg gagacgatca
    13801 agaaagagaa gatgaatctg accgccacga caggtgctgg cgttctggta cgacagggac
    13861 aaatgtccga cggcgtatgg ttctggacat tccagtatcc tgtccgtatg cgtctggtcg
    13921 gccagacaac cagcaagcca gaacagtctt ttgttttcga aatcaccatc cagcgtgttg
    13981 accccagact gaaaccatcc ggtatggaaa tccggcagat gatctcccgt aacgccggac
    14041 cgaactcctg agataagtaa tatgcaatca cgttatttac tgagcacact gctcctggtg
    14101 tgcagcgccg ccacgtctgc agataacgca ggatggcaga acgcacgcac accacaaacg
    14161 acaaattctg ccagcgatca cgttcagaac gcctcccaaa acaccggagg tgttccggct
    14221 acactggtaa aaggtgagct gcctgccccc gggcaggcca gcccgctggt acaggatgcg
    14281 gcaaggcttg attctgagct ctccgccgat gagattcgct cactacgcag tctcatggcg
    14341 gataacgagc gggccatcaa tgcgcccatc accagtgttg taccccgtat cagctctctg
    14401 acagttaatc tgtcccccgg tgccagcctt cctcttgttc gcacagccat gaacaacctg
    14461 agcgtggtga catttaccga tatcaacggt tcaccatggc cacaatccga tcctccttat
    14521 aacgcggcac caaaactctt tgatgtgcag tacaacgaga acatggtgac aatcacacca
    14581 ttgcggccat gggcatccgg aaacatttct gtctacctga aaggtctgtc ggtaccggtc
    14641 atcctgaacg taaccagtgg cgaaacagat acgccgtcat ccagtcagga aatggacagt
    14701 cgtctggatc tgcgaatacc acggcaggga ccaaccagcc cggttgtctc catcccgacg
    14761 gataaaatcg ccctgcatga tgcaacatta caggcatttc tggatggtat tcctccccgg
    14821 gacccttcag ttaagaggct gaaattcaca ggtaatgtcc cggatacaac gatctggcaa
    14881 cacggcgatg acctactggt tcgttcccgg gcgatgttgc gcgatgaatt tgaacagacc
    14941 ttgtcctctg ccgacggaac tcatctctgg aaacttcccg tcaccccact actgactttc
    15001 tctgtgaatg gccagtccgt tcacgtgaca ccagaactgg agtaatcatg agtgctgaac
    15061 aggatgccgg taaatccgga aagaaactgg ctgccctgct tggactgggc agcatcatct
    15121 tatttggcgg cggctatatc gcgttttcca aactatcagg gagtaacagc gatatgcagt
    15181 cagccgtgaa cattaacagt gcagcaagcg gcggaacgcg cagcgttacc gaaacgcctc
    15241 actatcgtga actgttaaga gcagataatg aacgaggggc cgcagctgca gcccgcaata
    15301 atcagacgtt cattgccagt cttccacagg ggcttgatat tccggactct caggaaaagc
    15361 aacaacagcc tgcagcaaaa ccggaaaatt atgctcatcg ccaggcttcc ggcacaccac
    15421 aggaggatcg agcagccagt gaaaaacgta tggagcggct gcaaaagctg attgtaagaa
    15481 taaaagatca gcatcctgcc ggtagtacac caaccatagc aacggctatg tggaataaat
    15541 ctccggcaga aacaacaggg cagaatggta cgcagcaatt cgcattacag aatgccagcc
    15601 tgtccacgcc ggtagcagaa aaagggatac aactgatacc tgctctgacc cgtataccgg
    15661 catatattga tactgcagtg gattctgata accagtcatc caaggttatc gcaaccattc
    15721 cggctggccc ctgggcaggg gcaacgctgt tcagtccggg agtcaaactg gtgggtaacg
    15781 gcgtggagat ccatttcgac cggatgagct ggaacggcat ggaccttaag gtcaatgcat
    15841 atgctcaacg ggaagacaac ctgatgtcat ccgtggccag caatgtgaat acacgctggt
    15901 ttaaacatat cattctgccg tcagtgttag gaggtgtggg cagtattggc acactttata
    15961 aagatgccaa cacgcaggtt attcagggta actatggcac ggttaccgga cgcgtgggaa
    16021 tgcccagtgg tgaagctgtt gcgggcgtta ttgccggtgg tatggcggaa agaggaagtc
    16081 agatattaac ccgccaggca gaaagtgagc cctataaaca ggtagaagtg tatcagcatg
    16141 aagtcgtatc aattctgttt gttgacccgg taatgacaaa cgacgccagg tcatcttccc
    16201 tttccagtgg catttcccct tcagttaatc gtacatcaca gacggaacaa cgctcgcagg
    16261 cacgaatgca gacagccatg gagcaaagaa aagctgtgat gcaacgtcgg tatgatgaac
    16321 aaccggagac accataatga aacctgaaac agaaatagat gaaaccggca gcttcggtga
    16381 acccgaagag aaacccgccc ctttctggaa acgttccgtc tgggggatat ctgtagcaac
    16441 atgggggcta tgtgcagtcg tattgctcgc agccatatgg tatctgttcc tgagagcacc
    16501 atcggaaaca ggcatgcctc cgtttaatga tgcggatgct ggcgtacaga cctggcaaac
    16561 cacacaagaa tcatcacctt ccgttcagtc cactgagacc atgactgtcc ggacaggaga
    16621 agatatgagc cagcttgccc gggatgtaaa aacagagctg gataaccgcg atgaaaaaat
    16681 tcaggcaaca ctgaatatgc ttcacgacag cattaacaag ctgggcgaag ctataaaaaa
    16741 ggatgaagaa tacgcacagg aaacgcgccg tcagctggat gatatccgga gccacctgaa
    16801 cggaatgatg acacagaaaa gcgtcaccga atcctcttcc acaccgcatc cagcaaagaa
    16861 aaaaacgtcg tctgtactta acggaatgaa aatcatgtcg atggaaactg gcatggtctg
    16921 gattcgctgg cagggtagta cctgggctgt cagagaaggt cagactctgg gcaatgtggt
    16981 gatccagcgg attgatccga cgacccggac catcatcacc agtgcaggga cgttacgctg
    17041 atgaacatgg atgccctgac cgccattgag aactttgcca gcagtatttt cagtgccgga
    17101 atggatttcc tgtttacctg gggagaattc atcggggtga tcagcatgat taccctgttt
    17161 gcccgcgccc gttcagccgg tccggtaaaa atgtctcccg gaaaatttat tgcggggatg
    17221 ttaaccagct gcatgctcgt atcactcccg gcaatgatta acgccggagg cgtacaaatg
    17281 ggattcaggg ctgactcatt tggcccgatt gcatatgtac aacctcagac gtttggcgct
    17341 gctgctggcg cagcaaatgc agttctgtcg ctggccaagc tggccggtgt gggatttgtc
    17401 atgaacggga tatccatctg gcgcaaggcc ggactcgatg gacataccgc actgagtgcc
    17461 agtgaatccg tcagtaaagg aaacgtcaaa tttattgccg gcgtcctgct ggtctttatc
    17521 gatcgtgtcc tcaatgccct gctggcaagc attggaatcg tcttctgatt tttttcacct
    17581 tcatataaac cactgatata tccatttaag gagaaactat gtcacgtatt cgtgtcgtgg
    17641 cggcccgtct gtacgccgca tttatccatg cacaactgtt tgtaatgtca tgccggagca
    17701 aactgactaa gtacctgctg ctcattccca tgatgttgct gcccaaagcc gttcttgccg
    17761 atggagatct tgccgatatg gttcgcaatg ttggtgaagg tgctaaaaca gcacagtcat
    17821 catcaatcac tattgcgcag ttcatcggcg taattctgtt tctcgggggc ctgattggcc
    17881 tgaaaaaagt tgggaaacaa ggggggatgg gactggctgg ttgcattgta tctatcgtca
    17941 ttggggcggt acttgtcgcc ggacctgaaa tgatgagtcg cagccagaaa cagttgggga
    18001 tttcctcaat ttccattggc taatcttgtc cagggcactg gcatttatcc ggtgcccttc
    18061 ctgttcggca atacaaggag gcacatatgt ctgcctggag cccggagcac ataacgttac
    18121 gacgaacaga aaacggcaat gtgattgccc acgacagtcg ggacgatcag gaattcattt
    18181 ttgcagaatg ctgttccgat gaaatcgcaa acatcattct ggaagccgtg aaacgttaca
    18241 ccagtacgga gcctgctcat gcaacacagc attaaagact tgtggttgta tccctttcca
    18301 gaaattgatg tggttcatac acaggagcca ctgttaccgg aaccggaatt aacaacgccc
    18361 ggtcgatgta tctgctgccg gcaaaacgtc aggcaccgtt tcagactgga tgactcatgg
    18421 ccactcagac agcttaccga cacaatcagt gacacacgag ttcgtcttaa taaggccaca
    18481 gaacatctgg ataagttaaa aaagcgtggt gaaccagtcg caaccggcga aaaggaaaag
    18541 tacaacacgg cagtaaaagc cgctgaacgg gcgcttgagc aggcccgtct gtctgccaga
    18601 cgtctttcac tccgccatgt acagaaagct gaaatcacca gtacagagtc gttgtcagag
    18661 aaagagcagg aactgtttca tgaagacggc ccgccttaca gcttatgtgc tttctgccac
    18721 gcctggcatt cactgaatgg ttatgccgca gcacagggcg ttatggtgtg gctacccgac
    18781 cttcatccat ccacggtcgt ggcccttaac cgtcgttcat tacaggaagt tttcagtaat
    18841 gataagttcc gggtccggcg gggacgggaa gcgctctctg cactgatgca aaacagactg
    18901 gcggttgagg ataaattccg cagtttccgc ccggccgatt tcgctgacgt ttttcgtcgc
    18961 tatccacctt ccggtcgttc gcccctgcgt gaaaaaatga acgggattgc cctgattctg
    19021 acaccagatt cattcatcaa aaaagaatac gttgattgat ttttcttcat gccactccag
    19081 cgcggggtgg cattaaaaaa atcaaactgc acaaacctcc ctcctgtaag tccggagttc
    19141 cctcacctat gaacataaac cgtttaatct tcactatcga agactgtctg aacacgctat
    19201 cccgtttctc tgtcgcaagc agttttgttg agtattgcga tttacgtact gtaattggtc
    19261 tggacagaca ggatcgtgaa cgtcgcccat ggctgaattc gccctatatt gcagccacaa
    19321 agcgtggaga atatctctct gtctttgaag tcagcggtgc tttcagggaa atggatgaag
    19381 catccgacca gactggccta ggttcactgg aatcgctgat caccagcatg agtgacagcc
    19441 tgaatacggc atataaaaac agcggtcata aaatcagctg cgtttttgag cgtgatcctg
    19501 acatggggaa agaagaaatc gaagacatgg tcgccccaca aaagcgctca ctggctaata
    19561 caggtatcca gttacaggat gtggtcgatg agaaagtcac aacactgtcc ccatggctgg
    19621 tccgggagcg ttgctggctg gcgatctgga gtggacccga ccttatcagc aacagcgatc
    19681 gcaccgccca tgatgagctt gtcagaagac tggctgaacg ggtaccgaaa gcacgctttg
    19741 cccagtcccc ctggcagtgg accttatctg cactgaaaat ccgccacgaa gcttttcttg
    19801 ataatgtgga acaggcactg agacattcat ctgatggcct gattctccgg ctactggata
    19861 tacacgaggt cgggcgtgaa atacgtcgtc agacagaacg gcacagcaca ccgcgtaact
    19921 ggcaacccca cctgcctgaa gatgcccagc cagcgggata tcgctggact gacgatgaat
    19981 ccgtactaca tgctccttca ttgcatttgc agttattcaa cacgcaggtg accacacagg
    20041 gcaacctggt tcaggcgggc ggactctggc acggaatggt atcgattact ttacctccgc
    20101 aaaatctgca gacgtttaac gaactggtcc gtgccgtccc ccgcgcagtc ccctggcgca
    20161 tcagaatgga tctgatgccc ggcgggatga aggctcttaa tctgaagaaa acgcttctga
    20221 cttacagcag cttcatttct gccgttcgtc caatgtatga atccgttatg accctggcgg
    20281 caacggatga aaaagaacct gtctgcataa tgaccatcat ggcctccacg tggggaaaaa
    20341 cacgtgaaat atgcacacga aaccaggcca ttctgaaatc ggccattgaa ggctggggcg
    20401 tatgtggcac cacgacaaca tttggtgatc cccgacgcgc ctgggtgaat accattctcg
    20461 cggcatccgg aggctccggc ccggttcccc tgtatccccc gttgtcacat gccatctcgc
    20521 ttttcccact taacagggca ggctctgtct ggcgaggcaa agggaatctg atgttacata
    20581 cagaagacgg ttccgcattt gaggttggtc tggcgtcatc ccaacaaaat aaacatacag
    20641 aactggcacc aggcgatccg ggactgggta aatcagttct gattaatacg ttgtcggaaa
    20701 tacagatatc atctgctcag aaaaacctgc ccttcattgc ctatatcgat aaaggataca
    20761 gtgctcaggg actggttcag cttattcgcg acagtcttcc tccggaaaga aaagacgaag
    20821 ctgtcggtat tattctgagc aatgatccgg aatacacccg taaccttttt gacgtgatgt
    20881 acggcgctaa aaagcctatt acgccggaaa agaatttcat gtcatcggtg ctgtgtgctc
    20941 tctgtgtcga taccggtacc ggtcaaccct gtaaccccgg agacacccga caaattatca
    21001 accagcttat cgagctggct tttaaagagt acggtgaaaa taatccgcga ctttatcgcg
    21061 cctcaacgga agacctggtg gattcagcac tgcaggactc gggactgtat gaaaaacatg
    21121 atgcagcctg gtgggccaga tcaacctggt tcgaagtccg ggatatgctc cacaatgccg
    21181 ggtatatcat ggcggctcag cgggcacatt atcaggcaat gccccagctt cccgaagtat
    21241 cctctatgct gggccatacc agtctcaggg atgttttcgg tactgttcag cgtgatggtt
    21301 caaacgaact tctgctggac tacatccgcc gcgccctgga gcagggacat aacgactacc
    21361 ccatgatatc cgggtatacc cggtttatga tcaacccgga aacccgggtc atcgctgtcg
    21421 atctaaataa tgttgccggt gacaaaacgc ctgcaggcag gcttaaaacc ggcatcatgt
    21481 acctgcttgc aggtcagatc gccggtggtg acttcaccct gccacaatac agggatgaag
    21541 tgttaaagca gcttccacgg gaataccatg aaatcgctct taaacgtatt aaccaacttg
    21601 atcaggaggt taagacaaaa gtttatgatg aactgcataa cgcccggggc attgacttca
    21661 tctgggaaaa tctggatacc caggagcgtg agcaacgcaa gtttgctatc cgcacggttc
    21721 tgagtactca gtatctgagg gactacccgg aaagtgttct taagtcagca aatactctct
    21781 ggttactgcg ctacaaaccc gaagatatcc ccgtactgag ggataacttc aacgtgcctg
    21841 aatttatgct gaaacgcttc ctgaaaatgc cggaaggccc ggcaccggat ggtagtggtg
    21901 ttcctgttct gggagtattc agggtaaaaa gtggcacgct tgcgagaata ctcaaattta
    21961 ccgtcggtcc actggagcta tgggcgctga actcatcacc aaaagacagt gccctgcgta
    22021 aaacgctgac aaataagtta ggttcagttc gggcacgtaa gattctggcc gaaaacttcc
    22081 ccagaggttc tgcaacgtca ttaattgagc accgggcggg ccaacataac agtgacaatg
    22141 ttattgaaga tctggcctcg gagctcatcc gaaaacaggg atacaatctc tgatgtgtac
    22201 aattcatatc accccacgct gccggagacc tgccagaggg caggtatttc ttaaaaagct
    22261 tgaggtaaaa ggtgacagtt tctcattcac tctgccgtca cgtctcttca ggttacatcc
    22321 ggcccccaat cccagagtga tatacgcttc ggatgccaga acacagggaa aggttacagg
    22381 catgcggctg ttgttgccgt ccagcacaga tgagctgata acaacggcaa tatggcaggt
    22441 ttctgtcccg gatgaagcac cattcgacgt tcagcatgtc atccagtgga tactcccttc
    22501 accgctggct tcgctgatgt ccgatgatac ctggttatgg ccatccgttc ccggaacgcc
    22561 ggcactgagt gccggtgact ggcctgttat ggatactgac ccttccacct tacggcgcgt
    22621 cagacgtcgt tctttcagca tcatcgataa ccggaacagg aaaggttata tcaccaggcg
    22681 atatcagtgt ttcccactgg ccccactacc tgaagacaga aaatacgaca gccttcctga
    22741 ccttgtgctc catcccgagc aggagacaat aaaatgcaaa gaaaaacatt actcgcagcc
    22801 cttatagcca ccctgagtgg aacggcatgc caggctcatg cttattcagt caccgtcgtc
    22861 gccagccggc ctgttgaaga gcaggtcatc cccagaatgg aagccatcaa agatgtcctt
    22921 ggcaacatac tgagtaccca gacagccacg ggtaccgcta tcaatcagaa ctctgaaaag
    22981 ctggcaagtg tcattgccca gaacggacag gcgacacgtc agcagatgat tttcagtaat
    23041 gaaactcagc gcctcgagga ggcacggaaa agcttcaccg tgccagactc catctgcagt
    23101 gaatccgcat cagggattgc aacagaaagc aaaagtgcct cagcatccgc agcctcaaaa
    23161 ctcagtaaag gtggcggtgt gagcaaccgt agcatccgcg accgtctggc cagcgcagca
    23221 aactctcccg tgcgggaagc ttatgatggc gcggccatac acgccagtta ctgtacagaa
    23281 gcagaatatg cacgttttgg aggaaccgcc gtttgtcctt ctgtgggcga aataccaggt
    23341 ggagacagtc aggtacgctc gatttaccac ggcgcaggta cagcggacac acctgcagca
    23401 ctgacatggg atcagaagca aatagatgca gcgacggctt acatgaaaaa cacttcccgc
    23461 ccctctgccg gtcgtgctct gggtaaagga gaagtgaata cccagagtgg gcgaacctac
    23521 gtcggactcc agaacgaata caacggcatc attgattctg cgtcgaatcc ccaactgacg
    23581 ctgattgctg acagcacgcc taatgaaagc acacgtaaag cactggctga aacactgcag
    23641 tcagactcag cggccgctta cttcgaccag gtggcttcac cagaagcaaa agcacgggga
    23701 tatatgagta cccgtgagtt tgaagctttt gaggctggcc ggcgctacgc caacacagca
    23761 tacctggtgg atttacagga aatgcaggga gataacctgc tccgtgaact ggtaagaatc
    23821 actgcgcaaa tgaactggca actgaacgac ctgaaagaac agatccgcca gggtaatgtc
    23881 atctctggac agcaactggc tctgaccgcc cgccagtatt atgaaaaaca actcggtagc
    23941 cttgagaaaa caatcaatca ggcaaacgca cgttgaaagc tgacgtcaga acaggactga
    24001 aaaaatgaat aagttaccgg aaaattcaga ccagacgaaa aacgtcaaaa aatcaccatc
    24061 cataacccgc atagccaccc gggggattat taacattttt gttcctctga tggagactcg
    24121 cctgataacg aattctgtac agcatatgct ggaacaacaa aaacgcaggc tggacagatt
    24181 tcgtcaggta aataataaaa aggcgcaact ccacctcacg tgggaagaag ctctgcaggc
    24241 cagccatatg tcagtagacg atctggatcg tcgtttccgc cggcgcagaa cagtatggcg
    24301 attctgttgc tggagtctgc tggccatcgc tttgtttctc agtggtatgc tctttgccgc
    24361 ctcttcatta cctcttacga ctctggtacg cgccatcagc actcttgtgt tgatactgtc
    24421 aggcgtcgct ctttgtgctt ccagagcact gattgttaca taccgcctct ggcagttgca
    24481 tgaacgaaaa gtcagcgaac ccgagcaggg gactttccgt gattttctga acgaccgcaa
    24541 tggctggcgc aatgccacat taattgccgt aaccagtaaa caatactgat cacgtccaga
    24601 tgccagtacc actggcatgt cgccatcccc cttaattaaa tcctgtgtgg cctttgccca
    24661 tacgcaattc tattccggag ttactgtgaa aattttactt cgcgcactct gtgcaggcct
    24721 tgctatatcc tcactgccgg caatggcttc agtgacctat caggatattg tgtctgccgc
    24781 aaccaatcct gacgatctgt ctcgccaggc gctggtcacg atattcggtg atgtagtcac
    24841 caatccactg tcaaccagcg cccccactct gatcggaagc atgttcgggg cgttcaacag
    24901 tatcatcgcc gtacttgccg ttgtctggtt tatgttcatt ggtatccgtc atgttgtgcg
    24961 ttcggggcat cagggacagg tattcagcac cggacgcgat atcgtcggca cactgagtgt
    25021 cgttgcaggt tttctgatga tcgttccaac cggaaatggc tggagtatcg ctcagctcat
    25081 tatgttatgg ggcgcatcga taatgggggt tgggtccgcc aatgtgatgg tgcaacttgc
    25141 cgctgataat atcgcaaatg gttattcaat gactgtacag ccagttcagg catccacacg
    25201 aacagcagcg cgtggcattt ttgaaatgga actgtgtaag tatgcggtta acgcaggact
    25261 aaatgatttt aatcagacgg tcaaatcctc gacttcactg atgacagaat cagctaaaac
    25321 cgccagtggt aattacactg tcacggtatc caatggcagc ggtatctgtg gttcagccag
    25381 cctgtcagta gaaggaaacg gaacaacaga tcaatcgacg attggaaaat tctttaatcc
    25441 cttcagtaaa aacgaataca gcggtgttat ctcagcacaa cgtgcagcca tggataatat
    25501 gattagcgac atggacaacg ctgccagtga gtttgtgaca acatttctgg aaaaaagaaa
    25561 ttctggcaac ggaacattgc cggatatcga gacacggatt cagagagcag ccgacgagta
    25621 tgaacgcgct gtacaaaaat cgctacctat tgataacgga gaacaatcca ggaaagaagc
    25681 tctcaagtct tatctcacaa cctatggatg ggtgacgctt ggcgcgtggt atcagacctt
    25741 tgccactgct aaccagcgtc tggcagagct ggctgacaga gctccagctg taacttcaat
    25801 gtcatcactt ggggaagttg gcgatactga tttatttagt gctgttatgg gggcatacaa
    25861 gactcaatta cagaactcca catatactcc aacgatagga acgataacaa ctcaagatga
    25921 aagtaatgca gctaatagca cagacccaca aagtgtggtc atgaaaagta tagcgccaac
    25981 catattaaaa tggacaaacc aagctgctac tgaatggtca ggaacaggaa caaccagcaa
    26041 ccaagttaac ccattaatta agatgaaaaa catcggcgat tatacattgg gtacaacaga
    26101 aattctctgg acaggatata caactgtcag agttctcgcg acaatgggtg ataactctat
    26161 tttcggaaag gctgttaatt tgttctctgg attacctaaa ggatttgttg ctctgctgga
    26221 tgctgcagct cccccaatat atttcttgtt gttcctgttg ttttgtgcgg gtttttcctt
    26281 atcaatttac ctaccattta taccattcat tttctggatg acaggcattg ggaactggat
    26341 agtaagcgta ttaataggat gcaccgccgg gcctctgtgg gcagcaacac atctaggaac
    26401 atcagaggac agaggaagtc gagcagctta cggatatata tacttaattg acagcatgat
    26461 aagaccacct ctcatggtat tcggtttctt ttttgcctct gtcgccatca ttgccgtcgg
    26521 cacaatatta aactcactgt ttggcgcagc actagttaac gtacaggtaa aatcattaac
    26581 agggatattc agccttgccg ggttcctgct aatttacgcc cgtatgtgta caacgtctgt
    26641 agctgcaata tttgctttac aagcatatct tcctgaccat gttatcaatt tcttgggtgg
    26701 tagagatggt gtaaatactc ttggcaacct cacctcatca gtaaaagata tttttgcagg
    26761 ttctaataga aatattagac acagccctgg agttagagag gacagactaa aaaatgtcac
    26821 aaaggatgat aataaagatg gaataaaagg ataattttaa taaaacaacc ttgccataaa
    26881 cactcaatcg cattaaaata tcgatgagtg tttatggagg gtttattatg caaatggtaa
    26941 aaacaatcaa tagatatcct gcttggtgga cattatatta cgtccttaga tttatatatt
    27001 tcataagcat accagtgtta tccattttta ttcttttcgg agtgctctcc attacatctt
    27061 caagatatgt gacgcaagaa gattatattt atacttgcgt ctgtctgttt ttactaattg
    27121 cccctgcaat attgatgtat tcccgagcaa gctcaagaaa agacaaaata aaaaaaattg
    27181 tcgcggagat aaaaaacacc ggtttttatt cacctgacaa agaatatgag ggactgtctt
    27241 ttactcaggg cgtctatttt ggcgtggata ccaaaaaagg cactatgctg tatgctcgtg
    27301 cttaccctgg aaatataatg gatattatcg gctttgacat tgacaacttc acacgcactg
    27361 tgaccgacgc caagacacta gagatttata caaaatacat aaatatacca atggtctcaa
    27421 ttccgtcagg atgcatccat cccaaaatga tggcggatac aatgcatgca atggcagaaa
    27481 gaggatatga ttaccctgtc gactttcctc gcctaattca ggagaagcgt aaggagtggg
    27541 agcagattgc aggcatgcct gtagccgaag tattttaaga taagctccct ccataacagc
    27601 cggttcaaat gagccggctt ttgttcatta caacagataa cttatgccgt taccgataaa
    27661 ttcgaccaag tgctttaatc gcagcaattt ttatttccat atcatcactt tgactcaatc
    27721 tatgtaattc acgagtaatg ttctccgcct gacaccggcc ttcacctaat gcatagatcg
    27781 cagcaagtgt aacgtctttt gatttactct tcatcatttc aaacaagaaa gtattagcta
    27841 tcgcaggagg catgctcata atcaatcctc ttcatgaaat aaacaatggc actcacttac
    27901 aaacaggagt ccactatcgc aagcgactca acttagcaat atctctcaat ttgaatgaaa
    27961 aaaacagatg gcatgtgaca gggaaccgtc cgcatgatgt ccgtgaccat ttaaccccat
    28021 aagctaacct tgtcacaaac gggcgcagaa cagcctgcca gacagacaac aaaacaaaag
    28081 tccaagataa aacatgaata ccggtatctc aatgcgaaga aataatctcc gtagaaaccc
    28141 ccatgagaaa aatatcatat gggagaaaat cgcaattatc acagccagcg cattcattat
    28201 tttcgctgtg ctctggcatt aattattttc aacacaaaaa ggaaaaagca atgaatctgg
    28261 tagacgcctt tgtcaaaaaa gtaatatccg ggccatataa agaatatgga aaatggtgga
    28321 tagatgttga atacatttca tggggcgttc ccgggaaaac aagactgatg tttgagtcaa
    28381 aggagcaggc actggaagtt aaagaagggt acaaattttt aacgtgagat ctctttttgt
    28441 taaagggcag gaagcggtaa ctccctgccc ttagttacgt ttccaggctt atcagtcgat
    28501 aagttcccgg atcagttcca gaacagcaat cagcgcgttt gcaacggcaa ctactgccct
    28561 gagaacgatg attaacagat taatcatccg cttctccatc actggagaaa gaaccccgtt
    28621 gttcgctctt acactgcctg tcacaacggt ttctatagtg tcagttatag acagttcttc
    28681 tccgaccaga gcaccaaaac cagcaccacc tgatgttttg gccaggactt gtaacctgtt
    28741 cttcccgctg aacaatatgc cggacagaac atgcctcaga gctgacatgc cgagtataca
    28801 cttatccagt acttgcagaa atataattcc agacgtacta tctacaggtc agttatagac
    28861 agttcttttc cgaccagagc caccaactct gtagcggaac agtaaaaaag ccgacctcac
    28921 cagtcggttt ttttacgtct gttgtttctc aaaatcctcc cgccatgccc gtcacaccgg
    28981 agaaactcat gctctgaatc agaaccaacc atctcaactt tgccgtctcc tgaaaaaact
    29041 ggcacattca gttcgataac tacccataaa tacagagaga aaatccgatg tcagaaaagt
    29101 ggagtaaaga attcgactct tgctttcagg tggggagcat agaagaaatt gccgatgctc
    29161 tcgagaagcg cagcggaagc gaggaaaacg cgttagccat gctggcaatg acagccttca
    29221 cactcatgac ccgacgtggt atctgcgaaa tgcagaacgt ggcccccgat ggcaaaggca
    29281 ttcggctgga gctaatcggc ttcagggaaa atgaaacaat accggatacg cttcattgaa
    29341 gaagaaaggg cgcaatgagg tcagccttcg caacaaagcg gcggcagaaa acctgccgcc
    29401 gtgttgcccg ttaacgttta acttcgtagg ctaacgttgc cacaagcact gtgtttccct
    29461 gctggagccg gagtccgcaa agcgaatccc tcaccatcca gacaaaacac agaatcgtca
    29521 cacagatgac gattaacatc attaaaaacg ttcgctgtgg catcatagcc cctccattgc
    29581 cttacggcgg ttatcgggct actataatgt tgtcgaagca ttacagtaga gcccttggtt
    29641 gattgataaa aaaatctcca ggggctttct gctgtatacc gtccacaaaa cgctttcggc
    29701 atacagcctc aagcgcccgc agctattcta acgactttcg tatctgcaac aatcctttta
    29761 tcgcacggct aattcctctg gttagctctt atcgcctgca aataaatttt taacctttcc
    29821 gtttgattta aattccagaa atggagactt tgtcatgtct tacaatcgac agcctgtcgc
    29881 agaagatcct atgcagatat ggggcgctgt tggtgtcctt cttatcctgc tgctattcgt
    29941 catatggtta ttcttgccgg aagtggttta cgccagctgc ctgattcttc ataccttatg
    30001 gggactggtc gactgggggc catttcataa ctatgcagca ccacgataca acctgcttgc
    30061 catgaccggt aacaacgccg ccaacataag ctatagccag tgggttaacg tcatggagca
    30121 gaccataggt attctgtgga tgtacctctt acctgtgaca ctctggtgtt tgtgggaatg
    30181 gtatcaacat ccgggacaaa gccgtttcac ccgcagacca gtagatatta cccgactgcc
    30241 acacatcttt gcttcgttgt cccctgctat cgcccctgta ctggcagatg gtgatccgga
    30301 aaaactcttt catggtggca aacgacctga acggcgggtt gctctcaccc cggaagcatt
    30361 tgtcgagcag catactctca tcaccaatat gcagctcgat gttgcagctg caagacgatg
    30421 tttcatggcc cagttgggta aacctttaac gtcctggaaa gacatggcac cacatgaaaa
    30481 agcgctcttt gccatatttg gtcttcagta tttcctggat gatcgtaagg cagcattaaa
    30541 gctcatggat acactgaacc tctcatgcag aattaaaagc aaacgggaca gtgggaaatt
    30601 ctgtacccct gtttattcac tggccaaatc agctttccag agagtgatta aaagcaacgg
    30661 agcacagcaa tggctgaaac aacatcgcta cgtccgcagt gggctggtct ggctgtatgc
    30721 acatgacctg cggcttacgc cacccaactg gatttggctg aaaggcgtgg acaggacgct
    30781 gttttatgca ttacaccgcg ccaataccac gaaaggattt attgaaggcg ctggcgtggt
    30841 ggccgttgca cgtgcagagg ccgaagccat gcgttttggt cttccctgcc ctgaaccctg
    30901 tgttgatgaa gcggtggaag gcttacggcg ggatatgctc agtctcggtc ttatctggga
    30961 tgaaccacaa ccagatcgcg accggaaacg ccgcatcctg accaactggt ccctcaccga
    31021 cgacatcctg ccccgcacac ctgctacaga caacgaattc tgattatccg gagaactgaa
    31081 aatgacaatc gagtactttg caacacgcat tgcaaaacat atttccgtgc aggcaatctg
    31141 gccgaatggc aggacagaaa ttattgccgt ccttccacgg gatgccctgt cagggctggt
    31201 aacacgaaat aatcaactga ttcatatagc ctttgacgcc atgggcaccc gtaacagaga
    31261 aaccgtcctg attgataaac aggagcataa cgctgaccag atacttacag ctattgattc
    31321 ctgcctgcgc cttgaatatc tgatggaacg ccgcttcagc agaacacctt tgttccgtgg
    31381 cataatcgcc agcgttgtgc tgttcgtaat ggcaacgatt ggcttctctc tgttccgcta
    31441 tgttgatcgt gtattctggg atgatacaac gcctgaagca gtacagacag caggtgagcc
    31501 acgactgctt ccccctcacc tgaatcacac tgtgccatta aatgaaggca ttcaactccc
    31561 ggttcccccg aaaaaagacg tccaggtacc ggagaaaaca atcacaagca aaaatccgga
    31621 agccgcagct gcgcgacata atctggctac tgttctgaag cggaacgccg atcggggcat
    31681 gtttaccatc aacctgtcat ccggtcatga acgaaccctg ttcgcatttc tcgatcctgc
    31741 ctgtcctaac tgcagacttc tggagccggc cttaaaacgc ctggccagcg acttcaatgt
    31801 cgttatctac ccggtttcag ttattggtgg agaggaatcc accgaccgtg tggcaccact
    31861 gctgtgtgaa aaagatgctc agaaacgtgc tgccggctgg caccgactgt attccgcaga
    31921 taacggaatg atgacgccat cagaagaaac aacgcctgca gatgaaacat gcctgaaagc
    31981 agccagagct gcgattgatg ttaacaatgt cgccttccgt aagttcggat ttgccggtac
    32041 cccatgggtg ctgtccgaca cgggctggca tctgcctact ggaattctgc aggaaactgg
    32101 cacgcttaat ttattcctga aaaccaccga cagcgagtct ggccatgagt gaacatcgcg
    32161 ttaaccccga gcttttacac cgtacagcct ggggaaatcc tgtctggaat gcactccaga
    32221 gtctcaatat ctatggcttt tgcctggtgg caagtcttgt ggccagtttt atctggccac
    32281 ttgcattacc ggcatgtctg ttgttcacac tgataacgat gctggtattt agtctgcagc
    32341 gatggcgctg ccctcttcgt atgcccatga cgctggaatg tgctgacccg tcacaggaca
    32401 gaatgattaa aagaagtctt ttcagcttct ggccaaccct gttccagtat gaggtcattc
    32461 tggaatcacc ggccagcggc atattctatg ttggttatca acgcgtcagg gatattggca
    32521 gggagttatg gctgagtatg gacgatctga cccgccatat aatgtttttc gccaccaccg
    32581 gtggcggtaa aacggaaacc atctttgcct gggcgattaa cccactttgc tgggcaagag
    32641 gattcaccct tgtcgatggc aaggcacaga atgatacggc aagaacgatc tggtatctgg
    32701 ccagacgctt tggacgtgaa gacgatgtgg aagtcattaa cttcatgaac ggaggaaaat
    32761 cccgcagtga aatcattctg agtggtgaaa aaacgcgtcc tcagtcaaat acctggaatc
    32821 ccttctgtta cagcactgag gcattcacag cggagacaat gcagtcaatg ctgccccaga
    32881 acgtccaggg gggcgagtgg cagtcccggg ccattgccat gaataaggca ctggtatttg
    32941 gtactaaatt ctggtgtgtg cgcgaaggca aaacaatgtc gctgcagatg ctccgtgaac
    33001 atatgactct cgaagggatg gccaaacttt actgtcgtgg cctcgatgat cagtggccgg
    33061 aagaagctat cgcaccgtta cgtaactacc ttcaggacgt tcccggtttc gacctgtcgc
    33121 tggtcaggac accatcagcc tggacggaag aacccagaaa acaacatgct tatctctccg
    33181 ggcagttttc tgaaactttc agcacattca cggaagcatt cggggatatt tttgctgaag
    33241 attccggtga tattgatatc cgtgacagca ttcacagcga caggattctg atggtaatga
    33301 tccccgctct ggatacatca gcgcatacaa catctgctct gggacgaatg tttatcaccc
    33361 agaaaagtat gattctcgcc cgtgatttgg gctaccgcct cgaaggaact gattccgatg
    33421 cactggaagt caaaaaatat aaggggcgtt tcccttatct gtgtttcctt gatgaagtgg
    33481 gggcatacta tacggaccgt attgctgtgg aagccactca ggtgcgttca ctggattttg
    33541 ctctgatttt gatggcacag gaccaggaac gtatagaagg acagacaaca gccacgaata
    33601 cggcaacact gatgcagaac acggggacaa aatttgccgg caggattgtt agcgagggta
    33661 gtacggccag aacgctgaaa agcgccgccg gtgaagaagc cagagcgaga atgaacaacc
    33721 tgcagcgtca ggacggcata tttggtgaat catggattga cagcccacaa atcagcatcc
    33781 tgatggaaag caagataaat gttcaggagt tgattgaact tcatcctggc gagttctttt
    33841 ctattttccg gggagaaacc gtaccatcag cctcattctt catccctgac gatgagaaaa
    33901 gctgcagcag cgatcccgtt gttataaaca ggtatatcag cgtggatgcg ccacgcctgg
    33961 accgtctccg ccgtcttgtt ccccgtacag ctcaacgtcg aatcccgtca ccggaaaatg
    34021 tcagtgccat cattggcgtc ctgactgcga aaccctccag aaaaagacga aaaattcgca
    34081 cggagccgca cacgatcgtg gatacattcc agcaacgcat tgcaggacgt caggcggcta
    34141 tggcaatgct ggaagagtac gacacggata tcaacgcacg tgaaagtgcc ttgtgggaaa
    34201 cagcggtgaa tacgcttaaa acgaccacac gggaagagcg acgaattcgc tatatcacgc
    34261 tgaacaggcc agaactacct gaaacaaaag aggaaaatca gatatcagtg agagcagaaa
    34321 gagcaggtat aaacctgtta acgcttccac aggacaataa ccacccgaca gggcgtcctg
    34381 taaacgggtt tcatcataaa aaaaccaacc gtccggactg ggacggaatg tactgaacag
    34441 tggccgcgcc ggattctggc gcggcctttt ttttaacctg gagaaggcgg ttcccagcgt
    34501 ttatcctgct ccggcggagg cagtgatgcg tcatcgttgc tgttcgccgc gatataatcg
    34561 cggtagtagc gttcacttcc ttcacggtga tgatccacgt ccggtcgcgt aatcctgtag
    34621 tcttcgtgac cggtacggcc tgtcacatta tgccaggcaa ccatttcagc aaagacctgg
    34681 ttaaattgtt tgtcgtttcc ttctggttca aaacccatgc gtggatcacg cccgaataat
    34741 accggtgcaa tcaaatccag tttatctgcc agcgcattgc ggtcatggtg attacggaaa
    34801 acaaccctgt caccataatc cgtacagaca aactctcccg tccgacgatc acggaaacga
    34861 acactcccgt tcttctgcaa acgggcctcg agatcaccgg tattaccgta taccggcgtt
    34921 ccgcccggtt cacaaagcac gacacagcgg tctgttgtcg ttgtgcgtga cttatcttta
    34981 cggcgatcgc gataatccca gcctctcagc tgcgatacag cagccctgtc tccctgagca
    35041 gcctgaattt cgacccactg acggtaagag ggtggatacc acttcccgtc agcaataagt
    35101 ttctgccgct cattcctgat gtcttctttc agcctgatca atgcctgcat tcgctgaact
    35161 tctgcaatgt gataatgcag cttacgtaat gccggatcat cgtactgcgc acgaatgtgt
    35221 gacttccgca gacggcatgc ctgatgaatt tcccgacaac gttccccata acgcagatct
    35281 ggtttacgcc attgttcacg ccatgccaga tagcgggcac gcaggtcagc acgggcctca
    35341 gctctggctt cacgtcgctg ccggcgcagc attgggtcac gtttactgct gaccccgtac
    35401 ctgtcactga cagccagctc cgggttatag cgactttcag gttgcaccct gtcaaacaaa
    35461 tctgctgggg cactcacaaa aggtccggcc tgtggctctg cacgccccag agtcagatca
    35521 ggatgaatgc tgctggcctt caccggcgtt tgctcatgat taaaggcatc aacgacaaca
    35581 agcccgtgat gttgtttctg tagcatcagt ccctgttgtg caaacaactc atgacaacgc
    35641 tgccaggtaa actcaccctg tgtgatcatt tcttcaatct gagggctaca atggctgatg
    35701 ctgtatcccc acagactttc tttgtcagaa aacagttcgc gtttgatagc cccctgcggc
    35761 cccgtacctg tacgcatacg gtgccattct ttatcggtcg caggacagct ctccatacga
    35821 cgaccacgga agcgttctgt tggcgtaacc tggaggaaaa tatctgttgg tacaggctgc
    35881 cagccaccat cgagttgatt cacattgtcg agcgtcagca gtgaccatac gctgtcagca
    35941 cgaacgggag tctggttatg atcataaccg tcacagataa ccaggtgacc atgctgaaca
    36001 cgcatccata atcccgcttc agccagtgtg cggtgaatag cctggcagtt ttccagccgt
    36061 ccttcccgtg ccatttccgg cagacgttct ccaagatgac gacaggcata ctgctgcaga
    36121 ctttctgttt cagcgatttt ttccgggcga acatcagctg caatgaagtc cggattatag
    36181 cgtcctggtg cttccacctg gctgaaaatg tcttttggta ctggcgtgta gtcacccatt
    36241 tttttcatga gtttttctgc acaccaggag ggaccaaacg agtcaagctg tacaccttcc
    36301 ctgttgcgat cccagccatc catcaccaga aattttccgt ccatctgaga caggtacaga
    36361 ccatcttccg caagacgacg atgcagggat aaccagtcat ttacaggttc actgcgtaaa
    36421 ccagcgaccg ctgtctgcgc gacatactcg cgaaaagttt gtttttttcc gcgtgtccaa
    36481 gcgttctggc gatcacgttc aacggcagtt ttgcgaacga tacgatttcc cggcgcatgg
    36541 acccagcaac cgttatccgg agcaaaacca tgcttcagtt caagctcacg gcaggcacga
    36601 ctgagttttt cctgactcca tgataatcgg ttcagataac ccgtttcagg gtgtacccgg
    36661 ttaacagcca catggacgtg cagattatct gtatcagtat gcacggcaga gacatactga
    36721 tggtcggcaa ggccgagtga tttcagcgta tgacgtacac tgtcataaat ctgttccgga
    36781 cgcgggcttt catgtgactg ccaggaaagg atgtagtgaa aaacaggatc agtgtcatct
    36841 tttgcatagt gtgcctgccg ggcaatgtac tccatatctg cagcggcagt ttcaagagaa
    36901 gtacagttat gaaagcaggt gacaccataa aagttgaccc attcgcagcc gtccttcatg
    36961 acatcaacca gcgccacgaa tgactcattt cgaagacgtg tcgcataatc aacaaggcga
    37021 ctgaaacggc ttcggtgcgg ttgttcagcc tgcgaagaag aagataaatc cagttcctca
    37081 tcggtcatgt catcccggac agagacgtag gataccaggt cttcgaacga agacttgccg
    37141 tcccttctct ttttcggaat gactgcattc accggagaat cctcaacctt ccattaactg
    37201 tttacgcaat gtatttgtaa gttcggttat ggcaacaagc acttctgcgt attccttgtc
    37261 accggttctt tttccctgaa caaacagatg cttctgcatc cttcccagtc tcattaactc
    37321 cttcagaaaa gcatcatctg tccgggagtt aattcgctta ttcagtgctg catttcggat
    37381 gtaagcagat acagtaagtc cggaatcttc agcattctta cagatagttt catcctcatc
    37441 cggtgagaac cggaccgctc tcaggatcgt cttttgccgt ctttcactac cacttctttg
    37501 acgtttatca ctcacggtca gctccttact ggggtgtcgg ggcgaagccc tgaccaggag
    37561 gcaattgtat aatcgcgcgt gcgcggttat acaattgcac atcctgtccc gtttttcagg
    37621 ccattatagc ctgttatttt tgtgcatcat agtcttatgc agacggcaga ggcagggcgc
    37681 agccctgcca ggaggcattt tttgatgata aaatcagact tcaacatgaa acggagagaa
    37741 agtgccaatg gcttctaaaa aattttactc agatgatgac atccagctgg caaaagctgc
    37801 tttatcggaa ttgccggacc tcaccgctca acggaaaaca ttgcgtgatt ttcttgatgc
    37861 cattcgtgat gacatcatta tcctggtaag gacaaagggt tatacactgg cagatgttcg
    37921 tgacacgctc aaaaatgcag ggtatgaagt aggagagaag gcacttcgtg acattattcg
    37981 ggaagcagaa tcgaaaaaac catcccgccg ttcttcctct aaaacagcac ctaaaaagac
    38041 agattcaggc aggaaagatg gcatcgatat gaacaacaat tgatgtgaca atgtcacatc
    38101 gtgttctgag attatgatga ttacagaaaa aaaaagcccc ggcataaata ccagggcttt
    38161 tctgtttaat cagactcgtg gcggcaatgc acctggataa tgccgtgcaa taatatcatt
    38221 cagtttttca acaaggtcag aacgcttaaa gacaatgtgt acggtccctt tctggtagcc
    38281 acgcaccgta aaatattccc cttctaacag cttaccgaca ttgtcgccgt tcccgagaaa
    38341 ttcaccatac tgcgccccga tgctgttcct gaagtcagga acgttgcgcc cctccagcag
    38401 ataaaacgga cgtaccaggt catccagtgc ctcccgtccg ctgcagtcca gcgtcacacg
    38461 cccattactc cagcggtaca gcagattttc aataatgatt ttcttaccca ggcggcaggg
    38521 attattggtt ctgtaatccc atgacaattt ccggtagaca tcaatcagtc cctgttcaaa
    38581 tgtctgcatc ttactggcat tcagatagcg aaaagttgcc agtacattgt ccagtgtgat
    38641 ttcaggacag gtgtcgctca tcagctggct gttccactca tcacgctggc atgaactcat
    38701 gaaggtgtac atgccagtat ccgtcatcag gcgatcccag attttccggt cggcaagacg
    38761 agtgatattc ttttttgctt cttccggttt atccatgcat cgaacggagt cagtcacgca
    38821 gtcttcaaac ccgtagagaa aaatgtcacc ggaagcatcg agcagaatcc tgcgcgctgt
    38881 cgccaggcat tccatcgcct gcatgaacag tgcaatacct tcgttacgct gcgcgatgat
    38941 gcggtcgata gccacagacg gaataacgtc tgtcgtgccg gcagtattca ggtcagcagt
    39001 gggtaaagtc tgattcatca cattgtctcc tcatcgtcac gactgccggg tcagccaggt
    39061 catttcccgg aggagcgaag ctccttctgc cgtccgggtc ttcggggatt tccccgcagt
    39121 ggacctttcc gacgggggac gctcaccacg tcacggggca gttgtccggg ggcagacgca
    39181 aaagtttttg acgcctgcgg caaaaacttt tgtggctggt acatctctcc cctggacgac
    39241 tgaaacggga tgtggtcgtg tatgtacccc cggaaagtcc gtctgcggag aacaggccgc
    39301 agtgcggcgg cagacaggca gagacagctg cgccatcagg cgcagtggcc ttacacaccg
    39361 taaggtgtgc agggagcgcc gcatccggcg cgactgaaga aaaacgacgg cacaacaatg
    39421 gcatgtggtg gtcactggca caccactccc gcacaggtga aaacagagaa aaccgcacga
    39481 catttacaac caaaaaaagt gaaatttctt cacttttttg cacttgttta tcgcgctgat
    39541 ttggcaattg tgatgataaa ataaagtgaa gttttttcac tttttcaaag gtgttttatg
    39601 actggctggg aactgcgaat ctggcgaaaa agcatgctct ggtcacgcga aaaagcggcc
    39661 agagaatttg gcgtgacgca gagaacatgg cacgcctggg aaaatgcgga acaggtggac
    39721 gtcaccgtct ggaggaccac ccaggcactt tcagtccgtg acctgctgcc tcacatgcag
    39781 gggatgcgca aggcagatat catcaggcgt ctggaaaatg aactggggga aacggcagag
    39841 gatgtgtgag cgtgattcac agcctggcag aatgccaggc tactgctcat caggaaacag
    39901 ttttttcagt tcttcaccgg aaagtccggc tgcctcctga atccgttccg gcgtccagcc
    39961 catatcccgc agccggcagg ctgcttcccg cgccacttca agcgcacctt tcttaaaacc
    40021 ttctttaaaa ccttcgtcga aaccttcgtc gaaacctttc tgtataagac gttctgcaat
    40081 cgtcatactg ttttctctgc tcctgctggc aaaaggcccg gtattttgcc gggctgtgag
    40141 gttctcatca gtgaatgatt ttcttcagtt cgtcatcgga aagtccggta gcctgcttaa
    40201 tctgctccgg cgtcatcccc attttctgaa gctggcacgc aatagccagg gctttttcaa
    40261 gcgcaccttc catcctgccc tcctggcgac ctttttgctc aagacgttct gcgatagtca
    40321 tcagtttatc ctcatgctgc ggcagacgct cagccagcgc ccgcataaat tccggtgata
    40381 cagagtcccc tgcgttaacg atgtaatgaa tcagaacacg cacctgctcg tctgaaattt
    40441 ccaccatgac ctgcgggagt ttgtccagca gctccatcat gtcacgatgg cggatatgct
    40501 tcatcagcag cgtcagtgcg gccatccggc gatggttcat gatttcattg tcatcaacga
    40561 cagtgatatc aaccagcgga aacggctttg tgtatatttt agccgcaagc gccggattct
    40621 caaaacagtc cagccagttc atgctgtacg gatacgggct gcgctcacca tgatagaaca
    40681 gcaccggaat caccagtggc aacgttttgt gctgctccag atgtctctgc atcgccgcca
    40741 cactgtaacg taacatgcgg aaaggcatgt acagattttc ggtgctctgg tgctcgatga
    40801 gcgtataaac atagccgtct ttacccccgg tgattttcac gctccagagg atatcgctgg
    40861 cgtactgatg caggtccggc tctacaaagg tggcgggttc cagcttcagc gtggacaggt
    40921 cgcacaactg ccggtactcc gcaggaagat gcagttccag aaaatctctg gcgacgtcgg
    40981 gattcgccag gaacgaccgg aacgcggcat catgcggcgt gggcgtcgtg gtgtttttct
    41041 tcttactcac ggttcctcca tcaaatcagc agcatcgcag caatcatcgc ccaaatcccc
    41101 acaaccgaga ggacagcaat tgcgttcaaa gccatgacaa caatagccgc gttctgagtc
    41161 acggcgtatt tcttcagcaa tctcttctgc ggaaaggtaa tcaaactcct cacgggccag
    41221 ttccaggacc agcagatgtt gttctgtcgg caatgtggcc acaaaattat caatagcagc
    41281 actcaaacgt cgctgatata gctcataaac aagaccacaa cggggcatta gttcttcgga
    41341 taatagttcc agttttgagc gaattctgtc agcgttgaaa gtgacttcat caaaagacat
    41401 atgaaaagct ttgtcagtat caaaacaaga cttcattgtt atctccagcc ctcccgggcc
    41461 ctgaatgaaa gagaatctgt ttcccttttc tgttttttgt ggagacacca tgcaacacag
    41521 acgtcgtgat gtttttcatc ttgctcacgg ttcctcctgg taaagtacct gaacacatcc
    41581 tgccacctgc acaggcagac ggcaggggtt aatcagtgac agacccgttc aatttcaagc
    41641 gcgcggagca ggtggcccca ttcgctgact ttcatggcgg ggatacgtac ccgcttctgc
    41701 tcactgcagc gtctggcgat atgaccgctc atcgccagga cgtccgggtt aatctcatcc
    41761 tgtgccagct cctgacgtct gaagcgggta tggcggtgca ggggtttatt ctcccgcagg
    41821 cactcttcac gcagacgctt tacatactcg gcggttgcga agataattga tggtctcatg
    41881 gcataatctc catagatagt gattgagatg ccgggcttcc tggcggggaa gcgccggcgg
    41941 agagaggcag ggtttatcgc cctgcctttt tttcatttcg gacagcggcg cagtgccagc
    42001 gcgagcgctt tcatcttcca gccttccggc gtgcgataca tcaccttcca gtagccgtcg
    42061 atgaacacat aatggaagcg cacctcttca gtgctttcag tggcaatagc ctccatgaaa
    42121 gaacggtact cacgcgcctc actgtccatc cggaaaccac tgtcacgtcc gtaataagcc
    42181 gtcacaccgt ccaccgggtt ctgaagcgta tgcccggagg gtttgtcgca acggggaccg
    42241 aggcaacgga tatcaccgtg ttcaaccagg gcttcggcct gctgaccagt ggcatagtgg
    42301 gtatgcagga tgcgaccgtt ccaggtcggc tcaccgtgca ggtggcagta aacggtacgg
    42361 aacaccccgt cgcggcaaaa aacggaaatc gtgctgctga ctgtcatgaa aacgtctcct
    42421 tctgctgaat caggtcgccg ggccgaccgg ttgctccccg gaggggcgaa agccccttct
    42481 gccgtccact tcttcggggt ttctccccgc agtggacctt tccgatgggg cgtccgtcac
    42541 cacgtcacgg ggcagctgtc cgggggcgga cgcaaaattt tttgccgggt ttatggctaa
    42601 aaattttgtg gacggtatat cttcccctgg actgctgaaa cgggatgtgg ttactccagc
    42661 ccccggaaag tccacagcgg gaaacaggcc gcaggggtgg cggcggaagg gttgactgcg
    42721 ccatcaggcg cagttgcctt acacaccgtc aggtgtgcag ggagcgccgt ttccggcgcg
    42781 acaggggcgg cgcgaccgga acggtcgctg caagcctggt ttttcag
//
LOCUS       DAEBXN010000027        41051 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.027, whole genome shotgun sequence.
ACCESSION   DAEBXN010000027 DAEBXN010000000
VERSION     DAEBXN010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 41051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41051
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.027"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..155
                     /locus_tag="KJL99_001454"
     CDS             <1..155
                     /locus_tag="KJL99_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBD4436235.1"
                     /translation="EQKMLQHTPIRRLGQPQDIANAALFLCSPAASWVSGQILTVSGG
                     GVQELN"
     gene            384..974
                     /gene="uidR"
                     /locus_tag="KJL99_001455"
     CDS             384..974
                     /gene="uidR"
                     /locus_tag="KJL99_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HBD4436236.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            1362..3065
                     /gene="uidA"
                     /locus_tag="KJL99_001456"
     CDS             1362..3065
                     /gene="uidA"
                     /locus_tag="KJL99_001456"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HBD4436237.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWCESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLSIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     N"
     gene            3071..4096
                     /gene="uidB"
                     /locus_tag="KJL99_001457"
                     /pseudo
     CDS             3071..4096
                     /gene="uidB"
                     /locus_tag="KJL99_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431055.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
     gene            4135..5400
                     /gene="uidC"
                     /locus_tag="KJL99_001458"
     CDS             4135..5400
                     /gene="uidC"
                     /locus_tag="KJL99_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HBD4436238.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIVGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMVGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWYDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGAN
                     YRVTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(5626..7134)
                     /locus_tag="KJL99_001459"
     CDS             complement(5626..7134)
                     /locus_tag="KJL99_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HBD4436239.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLGSFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(7235..8410)
                     /gene="manA"
                     /locus_tag="KJL99_001460"
     CDS             complement(7235..8410)
                     /gene="manA"
                     /locus_tag="KJL99_001460"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HBD4436240.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPHAERLSELFANLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            8609..10255
                     /gene="fumB"
                     /locus_tag="KJL99_001461"
     CDS             8609..10255
                     /gene="fumB"
                     /locus_tag="KJL99_001461"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HBD4436241.1"
                     /translation="MSNKPFHYQSPFPLKKNDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            10398..11801
                     /gene="fumC"
                     /locus_tag="KJL99_001462"
     CDS             10398..11801
                     /gene="fumC"
                     /locus_tag="KJL99_001462"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HBD4436242.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLDLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(11798..12727)
                     /gene="tus"
                     /locus_tag="KJL99_001463"
     CDS             complement(11798..12727)
                     /gene="tus"
                     /locus_tag="KJL99_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HBD4436243.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(12803..14104)
                     /gene="rstB"
                     /locus_tag="KJL99_001464"
     CDS             complement(12803..14104)
                     /gene="rstB"
                     /locus_tag="KJL99_001464"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HBD4436244.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALTMGGTVNCDISELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(14283..15602)
                     /locus_tag="KJL99_001465"
     CDS             complement(14283..15602)
                     /locus_tag="KJL99_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HBD4436245.1"
                     /translation="MLFLICSWGIFKTDQKSESLSKSVFIAIFLGAIIGISLHFIPET
                     SSETVIEWYNIAGNGYVNLLKLVAVPLIFISILSAINKLENSAGIGKMSLTIVACMLC
                     LVMIAGLVGLAIASAIGLDASAFGHMQSSLTSDDVSKTAAVSLPRLITSLIPTNLFLD
                     LTGARSVSVIGIVIFTILAGIALLKVKKDAPAEGEKLSAGINAIQIWVMKMVRIVIAL
                     TPYGVMALMAKVFSTYQLSQFTNLLGFIVACYLAVFVMFIVHSGILLLIGKNPVNYYK
                     TVWQVLTFAFVSRSSAASIPLAISALEKLGVPNTIANIAASFGSSMGQNGCAGIYPAV
                     MVAMIAPTMGIDPFSLQFLATLLPAIALGSIGVAGVGSGGTFAALIVLSTLNFPVALV
                     VIFIAIEPIVDMARTALNVNGSIMSGVISARLLKDRETKEQQSVTLN"
     gene            complement(15827..16570)
                     /gene="rstA"
                     /locus_tag="KJL99_001466"
     CDS             complement(15827..16570)
                     /gene="rstA"
                     /locus_tag="KJL99_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HBD4436246.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWDSDNKQSDD"
     gene            16699..17034
                     /locus_tag="KJL99_001467"
     CDS             16699..17034
                     /locus_tag="KJL99_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HBD4436247.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(17031..17753)
                     /gene="folM"
                     /locus_tag="KJL99_001468"
     CDS             complement(17031..17753)
                     /gene="folM"
                     /locus_tag="KJL99_001468"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HBD4436248.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(17790..19172)
                     /locus_tag="KJL99_001469"
     CDS             complement(17790..19172)
                     /locus_tag="KJL99_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBD4436249.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(19358..20302)
                     /locus_tag="KJL99_001470"
     CDS             complement(19358..20302)
                     /locus_tag="KJL99_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBD4436250.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQKRGNNLTVSADLYK"
     gene            20826..22358
                     /gene="pntA"
                     /locus_tag="KJL99_001471"
     CDS             20826..22358
                     /gene="pntA"
                     /locus_tag="KJL99_001471"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HBD4436251.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYIVPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            22369..23757
                     /gene="pntB"
                     /locus_tag="KJL99_001472"
     CDS             22369..23757
                     /gene="pntB"
                     /locus_tag="KJL99_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HBD4436252.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(23782..24816)
                     /gene="tqsA"
                     /locus_tag="KJL99_001473"
     CDS             complement(23782..24816)
                     /gene="tqsA"
                     /locus_tag="KJL99_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HBD4436253.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            25227..25592
                     /gene="mdtJ"
                     /locus_tag="KJL99_001474"
     CDS             25227..25592
                     /gene="mdtJ"
                     /locus_tag="KJL99_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HBD4436254.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            25579..25908
                     /gene="mdtI"
                     /locus_tag="KJL99_001475"
     CDS             25579..25908
                     /gene="mdtI"
                     /locus_tag="KJL99_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HBD4436255.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVVYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(25947..26768)
                     /locus_tag="KJL99_001476"
     CDS             complement(25947..26768)
                     /locus_tag="KJL99_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HBD4436256.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(26868..26951)
                     /locus_tag="KJL99_001477"
     CDS             complement(26868..26951)
                     /locus_tag="KJL99_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4436257.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(27044..27379)
                     /locus_tag="KJL99_001478"
     CDS             complement(27044..27379)
                     /locus_tag="KJL99_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HBD4436258.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHGHQQP
                     AKPAAQPAA"
     gene            complement(27776..29029)
                     /locus_tag="KJL99_001479"
                     /pseudo
     CDS             complement(27776..29029)
                     /locus_tag="KJL99_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            29136..30056
                     /locus_tag="KJL99_001480"
     CDS             29136..30056
                     /locus_tag="KJL99_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBD4436259.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPYDHPLANNPSVTLAELAELAELAELAKEPFVFFDPHVGTGLYDDI
                     LGLMRRYHLTPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDA
                     VSEMWLVWPKHHEQSPAARNFRIHLLNALR"
     gene            30191..31411
                     /gene="mlc"
                     /locus_tag="KJL99_001481"
                     /gene_synonym="dgsA"
     CDS             30191..31411
                     /gene="mlc"
                     /locus_tag="KJL99_001481"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HBD4436260.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVRELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGVGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIIIGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            31536..32231
                     /gene="bioD"
                     /locus_tag="KJL99_001482"
     CDS             31536..32231
                     /gene="bioD"
                     /locus_tag="KJL99_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HBD4436261.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKRVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(32184..33476)
                     /gene="clcB"
                     /locus_tag="KJL99_001483"
     CDS             complement(32184..33476)
                     /gene="clcB"
                     /locus_tag="KJL99_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HBD4436262.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAVFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSSWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAIVAWLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISQTLHRDSIYRQHTAQHS"
     gene            complement(33635..34249)
                     /gene="dmsD"
                     /locus_tag="KJL99_001484"
     CDS             complement(33635..34249)
                     /gene="dmsD"
                     /locus_tag="KJL99_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HBD4436263.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(34292..35146)
                     /locus_tag="KJL99_001485"
     CDS             complement(34292..35146)
                     /locus_tag="KJL99_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HBD4436264.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAEVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(35148..35765)
                     /gene="dmsB"
                     /locus_tag="KJL99_001486"
     CDS             complement(35148..35765)
                     /gene="dmsB"
                     /locus_tag="KJL99_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HBD4436265.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(35776..37814)
                     /locus_tag="KJL99_001487"
                     /pseudo
     CDS             complement(35776..37814)
                     /locus_tag="KJL99_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(37875..38984)
                     /locus_tag="KJL99_001488"
     CDS             complement(37875..38984)
                     /locus_tag="KJL99_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBD4436266.1"
                     /translation="MVNVSHIRTLSSVESTTSSPSIRLTVESIWNYAGNTLVNQHSDI
                     NKTHEILQDESKCEMIVVIENFMTSSAKYADILLEDLMTVEQEDIIPNDYAGNMGYLI
                     FLQPVTSEKFERKPIYWILSEVAKRLGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPAL
                     PSYDELKKMGIYKRKDPNGHFVAYKAFRDDPEANPLKTPSGKIEIYSSKLAEIARTWE
                     LEKDEVISPLPVYASTFEGWDSPERRTFPLQLFGFHYKSRTHSTYGNIDVLKAACRQE
                     VWINPIDAQKRGIANGDMVRVFNHRGEVRLPAKVTPRILPGVSAMGQGAWHEPNMSGD
                     KIDHGGCVNTLTTLRPSPLAKGNPQHTNLVEIEKI"
     gene            38967..40556
                     /locus_tag="KJL99_001489"
     CDS             38967..40556
                     /locus_tag="KJL99_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBD4436267.1"
                     /translation="MTDINHDNTAYPSSYFSGSPYLYGQVYTPSPDTRLTQINLLGVA
                     KPQLVRRANGRYTIRFRLKGQTTPFLSVSTRSTDRRVATMRQRELAATAKAFMLDRPE
                     VSLQELTEHLRSMAEQFLTDASDDYWNGLEVATLVDEKSNLKELAATQALSLDQQKGI
                     RLALEVLTAAQQRVDTGDTSGLIKLIDDNNHTDYSTIGDSTSILKNEQGDRPVVFTQE
                     RQPEVTICSFSELVSSLLAEKVQTLKSSSYKDLSSSLNTVSRFLPEDMDLMSRSEWLA
                     VRDAMLASEVRPSTINKLLTKAKMCLDYGLMNGQLEGRNPIERMKLTKDVDSKRRAFT
                     DEEMERLLVRVESEYQFTRHTAHTTSEARRWASLVSVVTGARSAEVCHLTKRDIVTLD
                     NGLVCIDINEDGDGKSVKNKHSVRLVPLTDGACGLDLKSFLEWVDMQPDEGPLFGMTP
                     SAYSSWFNSRVLTEALGDATDVSLHSLRHWLATRMKERGANLVDAQGILGHSSQSITY
                     DLYGKGHAVGRLAEVLKTALL"
BASE COUNT        10419 a         9965 c        10521 g        10146 t
ORIGIN      
        1 ttgaacaaaa aatgttacag cacacgccga tcagacgtct gggccaaccg caagatattg
       61 ctaacgcggc gctgttcctt tgctcgcctg cagccagctg ggtaagcgga caaattctca
      121 ccgtctccgg tggtggggta caggagctca attaatacac taacgaaccg gtaaacggtc
      181 gtatgtgttg tttaccggga tagcgtgtgc aattcatcaa cgtctctgct aaacaactgg
      241 cagccaaatc acggctattg gttaaccaat tgctgggtga aaagtatacg aatagagtgt
      301 accttcgcac tattcaacag caatgatagg cgctcacctg acaacgcggt aaactagtta
      361 ttcacgctaa ctataatggt ttaatgatgg ataacatgca gactgaagca caaccgacac
      421 ggacccggat cctcaatgct gccagagaga ttttttcaga aaatggattt cacagtgcct
      481 cgatgaaagc catctgtaaa tcttgcgcca ttagtcctgg gacgctctat caccatttca
      541 tctccaaaga agccttgatt caggcgatta tcttacagga ccaggagagg gcgctggctc
      601 gtttccggga accgattgaa gggattcatt ttgttgacta tatggtcgag tctattgtct
      661 ctctcaccca tgaagccttt gggcaacggg cgctggtggt cgaaattatg gcggaaggga
      721 tgcgtaaccc acaggtcgcc gccatgctta aaaataagca tatgacgatc actgaatttg
      781 ttgcccagcg gatgcgtgat gcccagcaaa aaggcgagat aagcccagac atcaacacgg
      841 caatgacttc acgtttactg ctggatctga cctacggtgt actggccgat atcgaagcgg
      901 aagacctggc gcgtgaagcg tcgtttgctc agggattacg cgcgatgatt ggcggtatat
      961 taaccgcatc ctgatcctct catttcgcgg gctggtgata actgtgcccg cgtttcatat
     1021 cgtaatttct ctgtgcaaaa attatcctcc ccgtgttcgg agaattcccc cccaaaatat
     1081 tcaccgtagc catatgtcat gagagtttat cgttcccaac actctcgaac gaacgttcga
     1141 ttgcttattt tatggcttct gtcaacgctg ttttaaagat taatgcgatc catatcacgc
     1201 tgtgggtatt gcagtttttg gttttttgat cgcggtgtca gttcttttta tttccatttc
     1261 tcttccatgg gtttctcaca gataactgtg tgcaacacag aattggttaa ctaatcagat
     1321 taaaggttga ccagtatttt tatcttaatg aggagtccct tatgttacgt cctgtagaaa
     1381 ccccaacccg tgaaatcaaa aaactcgacg gcctgtgggc attcagtctg gatcgcgaaa
     1441 actgtggaat tgatcagcgt tggtgcgaaa gcgcgttaca agaaagccgg gcaattgcag
     1501 tgccaggcag ttttaacgat cagttcgccg atgcagatat tcgtaattat gtgggcaacg
     1561 tctggtatca gcgcgaagtc tttataccga aaggttgggc aggccagcgt atcgtgctgc
     1621 gtttcgatgc ggtcactcat tacggcaaag tgtgggtcaa taatcaggaa gtgatggagc
     1681 atcagggcgg ctatacgcca tttgaagccg atgtcacgcc gtatgttatt gccgggaaaa
     1741 gtgtacgtat caccgtttgt gtgaacaacg aactgaactg gcagactatc ccgccgggaa
     1801 tggtgattac cgacgaaaac ggcaagaaaa agcagtctta cttccatgat ttctttaact
     1861 atgccgggat ccatcgcagc gtaatgctct acaccacgcc gaacacctgg gtggacgata
     1921 tcaccgtggt gacgcatgtc gcgcaagact gtaaccacgc gtctgttgac tggcaggtgg
     1981 tggccaatgg tgatatcagc gttgaactgc gtgatgcgga tcaacaggtg gttgcaactg
     2041 gacaaggcac cagcgggact ttgcaagtgg tgaatccgca cctctggcaa ccgggtgaag
     2101 gttatctcta tgaactgtgc gtcacagcca aaagccagac agagtgtgat atctacccgc
     2161 tgcgcgtcgg catccggtca gtggcagtga agggcgaaca gttcctgatt aaccacaaac
     2221 cgttctactt tactggcttt ggtcgtcatg aagatgcgga tttgcgaggc aaaggattcg
     2281 ataacgtgct gatggtgcac gatcacgcat taatggactg gattggggcc aactcctacc
     2341 gtacctcgca ttacccttac gctgaagaga tgctcgactg ggcagatgaa catggcatcg
     2401 tggtgattga tgaaactgca gctgtcggct ttaacctctc tttaagcatt ggtttcgaag
     2461 cgggcaacaa gccgaaagaa ctgtacagcg aagaggctgt caacggggaa actcagcagg
     2521 cgcacttaca ggcgattaaa gagctgatag cgcgtgacaa aaaccaccca agcgtggtga
     2581 tgtggagtat tgccaacgaa ccggataccc gtccgcaagg tgcacgggaa tatttcgcgc
     2641 cactggcgga agcaacgcgt aaactcgacc cgacgcgtcc gatcacctgc gtcaatgtaa
     2701 tgttctgcga cgctcacacc gataccatca gcgatctctt tgatgtgctg tgcctgaacc
     2761 gttattacgg ttggtatgtc caaagcggcg atttggaaac ggcagagaag gtactggaaa
     2821 aagaactgct ggcctggcag gagaaactgc atcagccgat tatcatcacc gaatacggcg
     2881 tggatacgtt agccggcctg cactcaatgt acaccgacat gtggagtgaa gagtatcagt
     2941 gtgcatggct ggatatgtat caccgcgtct ttgatcgcgt cagcgccgtc gtcggtgaac
     3001 aggtatggaa tttcgccgat tttgcgacct cgcaaggcat attgcgcgtt ggcggtaaca
     3061 attgacctac atgggcctcg ggctttgcta cagcctggtg aatattcctt atggttcact
     3121 tgctaccgcg atgacccaac aaccacaatc tcgcgcccgt ctgggcgcgg ctcgtgggat
     3181 tgccgcttca ttgacctttg tatgcctggc atttctgata ggaccgagca ttaagaactc
     3241 cagcccggaa gagatggtgt cggtatacca tttctggacg attgtgctgg tgattgccgg
     3301 aatggtgctt tacttcatct gcttcaaatc gacgcgtgaa aatgtggtac gtatcgtggc
     3361 gcagccgtca ttgaagatca gtttgcaaac cctgaaacgg aatcgcccgc tgtttatgtt
     3421 gtgcatcggt gcgctgtgtg tgctgatttc gaccttcgcg gtcagcgcct cgtcgttgtt
     3481 ctacgtgcgc tatgtgttaa atgataccgg gctgttcact gtgctggtac tggtgcaaaa
     3541 cctggttggt actgtggcat cggcaccgct tgtgccgggg atggtcgcga ggatcggtaa
     3601 aaagaatacc ttcctgattg gcgctttgct gggaacctgc ggttatctgc tattcttctg
     3661 ggtttccgtc tggtcactgc cggtggcgtt ggttgcgttg gccatcgctt caattggtca
     3721 gggcgttact atgactgtga tgtgggcact ggaagctgat accgtagagt acggtgaata
     3781 cctgaccggc gtgcgaattg aagggctcac ctattcacta ttctcattta cccgtaaatg
     3841 cggtcaggca atcggaggtt caattcctgc ctttattttg ggattaagcg gatatatcgc
     3901 caatcaggtg taaacgccgg aagttattat gggcatccgc acatcaattg ccttagtacc
     3961 ttgcggattt atgctactgg cattcgttat tatctggttt tatccgctca cggataaaaa
     4021 attcaaagaa atcgtggttg aaattgataa tcgtaaaaaa gtgcagcagc aattaatcag
     4081 cgatatcact aattaatatt caataaaaat aatcagaaca tcaaaggtgc aactatgaga
     4141 aaaatagtgg ccatggccgt tatttgcctg acggctgcct ctggccttac ctctgcttac
     4201 gcggcgcaac tggctgacga tgaagcggga ctacgcatca gactgaaaaa cgaattgcgc
     4261 agggctgata agcccagtgc tggcgcggga agagatattt acgcatgggt acagggagga
     4321 ttgctcgatt tcaatagtgg ttattattcc aatattgttg gcgttgaagg cggagcgtat
     4381 tatgtttata aattaggtgc tcgtgctgat atgagtaccc ggtggtatct tgatggtgat
     4441 aaaagttttg gctttgccct gggggcagta aaaataaaac ccagtgaaaa tagcctgctt
     4501 aaattaggtc gcttcgggac ggattatagt tatggtagct taccttatcg tattccgtta
     4561 atggttggca gttcgcaacg tacattaccg acagtttctg aaggagcatt aggttattgg
     4621 gctttaacgc caaatattga tctgtgggga atgtggcgtt cacgagtatt tttatggact
     4681 gattcaacaa ccggtattcg tgatgaaggg gtgtataaca gccagacggg aaaatacgat
     4741 aaacatcgcg cacgttcttt tttagccgcc agttggtatg atgataccag tcgctattct
     4801 ctgggggcat cggtacagaa agatgtttcc aatcagatac aaagtattct cgagaaaagc
     4861 ataccgctcg acccgaatta tacgttgaaa ggggagttgc tcggctttta cgcgcagctc
     4921 gaaggtttaa gtcgtaatac cagccagccc aatgaaacgg cgttggttag tggacaattg
     4981 acctggaatg cgccgtgggg aagcgtattt ggcagtggtg gttatttgcg ccatgcaatg
     5041 aatggtgccg tggtggatac cgacattggc tatccctttt cattaagtct tgatcgtaac
     5101 cgtgaaggaa tgcagtcctg gcaattaggt gccaactacc gtgtaactcc gcaatttacg
     5161 ctgacatttg caccgattgt gactcgcggc tatgaatcca gtaaacgaga tgtgcggatt
     5221 gaaggcgcgg gtatcttagg tggtatgaac tatcgggtca gcgaagggcc gttacaaggg
     5281 atgaatttct ttcttgctgc cgataaaggg cgggaaaagc gcgatggcag tacgctgggc
     5341 gatcgcctga attactggga tgtgaaaatg agtattcagt atgactttat gctgaagtaa
     5401 gaaataacgc cggagagaaa aacctccggc gtttcagatt gttgacaaag tgcgcgtttt
     5461 ttatgccgga tgcggcgtaa acgccttatc cagcctacaa aaactcataa attcaaagtg
     5521 ttgcaggaaa aggtaggcct gataagcgta gcgcatcagg caatctctgg tttgttttca
     5581 gatgaaaacg ccggagtgaa aattctccgg cgttttggcc gtgaattact gctgcggaat
     5641 tgccggtaca gccggaacgt taagagctgg catcgcaaac atgccaacaa aatcttctaa
     5701 cgacattttc tgcccattta acgttatctg accgttagca tattgcaggc tggtggtgat
     5761 ggtattgtcc tgcaaggtgg tcagacggaa catctgcccc attgctgatg caccttcaac
     5821 ttgctgtttc gccagttttt tcgcttgatc ttcctgataa ccttccagct tcgctacctg
     5881 agtcatcaac tcagttgcca tatccaccgg aatggtcagt ttcgcatcca gagatttaac
     5941 cgaacgatct acttcctgcg ccagcgtttg cggcgcttct ttagtcgttg ccggatcttt
     6001 caggaacagc gacagattca gcgcactttc accctgactg tttttccagc ttagcggcgc
     6061 gatagtaatc accggatcgc ctttcaacat cagcggcagg gcgctaaaga aggcttccgt
     6121 cactttctcc tgataaagtt cgggattgtt ggcaatttct ggctgtgcca gcagcgcctg
     6181 agtttgcgcg ttatattgct ggctaaactg atgccaggct tcaccatcaa tctggccgac
     6241 ttttaaagtc agcttgccgc tgcccagatc ctgattctgt accttcaggc tgtttagcga
     6301 gtaatccagt tggctattga tcgttttacc gtcattgacc agatccgatt taccgctgat
     6361 ctccatgcct tccagcagtg ccagttcttt gccttccact gaaatggtca ttttttccag
     6421 tgacagtttt tggtttccta cgcgctcacc aaaacttccc agcgtgctgg aaccgtcggt
     6481 tttcagatta ttaaaggtca actgcacttt ctggttgtat tcgttaactg cgtctatccg
     6541 accactttgc gcctctccgg aaagggagat ggctttgccg tctctgtcag catttaactg
     6601 gaactcgccg ccgctaaagg cgactttttc atccttttgc tcgtaattca gtggattgag
     6661 cgaaatatcg gaactggaat caccgctgta accaatgcgc gagttaatct caaaaggcgt
     6721 ttcacctttt gccatatcaa acagtggttt gcttacttcg ttattaacca gcgtggtttg
     6781 aattgatgcc atcgacggga tcaggttcag ttttttaagc tgggcaagcg ggaagggacc
     6841 atgatcaacc gattcgttga agatgacgct ctgaccgctt ttaatccacg gattttcttt
     6901 cccggcaatg ggtttcacca acagttgcaa ctggctgctg aatacgccgc gatgatagtt
     6961 ttgataactc acttccaggt tggattcagg agctgtcagt ttgagttgcg cgttcgcctg
     7021 cgcgaccatg tcttcgagat gggtttcaat cttcttgcct gtataccatg cgccgcctgt
     7081 ccagactacg cctagcgcaa caatgacgcc taccgctacc agcgatttat tcataatgat
     7141 tatccataaa atgaaatcag gcggactggc cgcctgaagg tgttataagc ctttaataag
     7201 cttagcaaga gatgttaatt ttttcagtaa gctcttacag cttgttgtaa acacgcgcta
     7261 aacggccgtg gcctttgaca gtcaccggtg attcgttggc ggcaataaac gctgattcac
     7321 ccggtttaag ctgtaactgc tgagaacctt tccacaacgt tgcatcgcct tcgacgcaga
     7381 acaaaatggc ggcactctgc tggctgatgg tggtttcttt atcactaagg tcatgcagcg
     7441 agaaggcaaa atcatccaca ggaatcggga agtccagttc tgcaccttgt ttcaccggct
     7501 gggtcaacaa ctggttagcc ggtttggctt cgaatttcac attggcaacc agttccggaa
     7561 tatcaatgta tttaggcgtc agacctgcac gcagcacgtt atcggagttc gccatcactt
     7621 ccagcgccac gccttgcagg taagcgtgcg gtgtttcagc gaacaggaac atcgcttcgc
     7681 caggattcaa tttcaccaca ttcagtaaca gcggggagaa caggccgctg tcttccgggt
     7741 aaaattcaga aattaaacga atcgtttgcc acggttcacc ctgctgactg tcgagcgccg
     7801 attttaaaat cgccagcgcg cgggattttt cttcaccctg catattcaac aggttggcga
     7861 acagttcgct taaacgttcg gcatgtggct gttgtaaaaa gtgagcaatc gccggatgtg
     7921 caccggcgac cggctggagt agggagacaa tctcggaaaa ttcacgaaac gcgttcatcg
     7981 caaggaaagg cgtcagcgca aaaaccagct ccggcttgtg gttaggatct ttatagttac
     8041 gctcggcggc atccatcggg atacctgcgg cattttcttt ggcaaaaccg atttcagaat
     8101 tgtgtttgtt tggatgaacc tgaatggaga gtggctgtgc tgcgcataat actttgaaca
     8161 ggaaaggcag ttcgccaaag cgtttggcaa cggcctctcc gagcagagtc gatttatcac
     8221 tctcaatcac atcacgcagt gaaacgatat ctccggcggc attctgcact cgtgaactgc
     8281 ttttcggatg tgcgcccatc cacagctcgg ccatcggctg gctggacgga ttttccatac
     8341 cataaagttc agtcaacgcc gttttgctac cccaggcata gttttgcact gagttaatga
     8401 gtttttgcat gatcaatccc tgttttaatg tggaaattaa tcccgctatt aaagcaagaa
     8461 tcctacggga agtaacctgg agccgcaaaa agtcgtacta gtctcagttt ttgttaaaaa
     8521 actgtgtagg atattgttac tcgcttttaa cagggcaacg gaacacccgc ccagagcata
     8581 accaaaccag gcagtaagtg agagaacaat gtcaaacaaa ccctttcatt atcagtctcc
     8641 ttttccactc aaaaaaaatg atactgagta ttacctgcta accagtgaac acgttagcgt
     8701 atctgaattt gaagggcagg agattttgaa agtcgcaccc gaagcgttaa ctctgttggc
     8761 gcgtcaggcg tttcatgatg cgtcgtttat gctgcgtccg gctcaccaac aacaggtggc
     8821 cgacattctg cgtgacccgg aggccagcga aaatgataaa tatgtggcgc tgcaattcct
     8881 gcgtaactcc gacatcgcgg cgaaaggcgt tctgccaacc tgtcaggata ccggcaccgc
     8941 gattattgtt ggtaaaaaag ggcagcgtgt atggaccggt ggtggtgatg aagcggcgct
     9001 ggcgcgcggt gtctataaca cttatatcga agataatctg cgctactcgc aaaacgcgcc
     9061 gctggatatg tataaagaag tgaataccgg caccaatctg ccagcgcaga tcgatcttta
     9121 tgccgttgat ggcgacgaat acaaattcct ctgtatcgca aaaggtggtg gttcggcaaa
     9181 caagacgtat ctctatcagg aaaccaaagc gttactgacg ccggggaaac tgaaaaatta
     9241 cctggttgag aagatgcgca cgctgggtac cgcggcctgt cctccgtatc atattgcgtt
     9301 cgttattggt ggaacttctg cagaaacgaa ccttaaaacg gtgaaactgg cttccgcgaa
     9361 atactatgat gaactgccaa cggaagggaa tgagcacggt caggcgttcc gcgatgtgga
     9421 actggaaaaa gaattactga tcgaagcgca aaatctcggt ctgggtgcgc agtttggtgg
     9481 taaatacttc gctcacgaca tccgcgtgat tcgcctgcca cgtcacggcg catcctgtcc
     9541 ggttggtatg ggtgtttcct gctctgctga ccgtaatatc aaagcgaaga tcaaccgtca
     9601 ggggatctgg atcgaaaaac tggaacataa tccaggtaaa tatatccctg aagagctgcg
     9661 taaagcggga gaaggcgaag cggtgcgcgt tgaccttaac cgtccgatga aagagatcct
     9721 cgcacagttg tcgcagtatc ccgtttctac acgcttatcg cttaacggca cgattatcgt
     9781 tggtcgtgat attgctcacg ccaaactgaa agagcggatg gataacggtg aagggctgcc
     9841 gcaatacatt aaagatcatc cgatttacta cgcgggtccg gccaaaacgc cggaaggtta
     9901 tgcctccggt tctcttggcc caacgaccgc cggacggatg gattcttatg tcgatcaact
     9961 gcaagcgcag ggcggaagta tgatcatgct ggcgaaaggc aaccgcagcc agcaggtgac
    10021 ggatgcctgt aaaaaacacg gcggcttcta ccttggcagt atcggtggtc cggccgctgt
    10081 attggcgcag ggaagtatta agagcctgga atgtgttgaa tatccggaac tgggaatgga
    10141 agccatctgg aaaattgaag tggaagattt cccggcgttt atccttgtgg atgataaagg
    10201 aaatgacttc ttccagcaga tacaactcac acagtgcact cgctgtgtga aataaacaga
    10261 gccgcccttc ggggcggttt ttttacatgg cacgaaagac caaacatttg ttatcaaata
    10321 gtaaataatg agtgagctaa aagttgctta acgaaagcaa aacagaaaga aaaaattaat
    10381 caggtgagga gcaggccatg aatacagtac gcagcgaaaa agattcgatg ggggcgattg
    10441 atgtcccggc agataagctg tggggcgcac aaactcaacg ctcgctggag catttccgca
    10501 tttcgacgga gaaaatgccc acctcactga ttcatgcgct ggcgctaacc aagcgcgcag
    10561 cggcaaaagt taatgaagat ttagacttgt tgtctgaaga gaaagcgagc gccattcggc
    10621 aggcggcgga tgaagtactg gcaggacagc atgacgacga attcccgctg gctatctggc
    10681 agaccggctc cggcacgcaa agtaacatga acatgaacga agtgctggct aaccgggcca
    10741 gtgaattact cggcggtgtg cgcgggatgg aacgtaaagt tcaccctaac gacgacgtga
    10801 acaaaagcca aagttccaac gatgtctttc cgacggcgat gcacgttgcg gcgctgctgg
    10861 cactgcgcaa gcaactcatt ccgcagctta aaaccctgac acagacgctg agtgaaaaat
    10921 cgcgtgcatt tgccgatatc gtcaaaatcg gtcgaaccca cttgcaggac gccacgccgc
    10981 taacactagg gcaggagatt tccggctggg tagcgatgct cgagcataat ctcaaacata
    11041 tcgaatacag cctgcctcac gtagcggaac tggctcttgg cggtacagcg gtgggtactg
    11101 gactaaatac ccatccggag tatgcgcgtc gcgtagcaga tgaactggca gtcattacct
    11161 gtgcaccgtt tgttaccgcg ccgaacaaat ttgaagcgct tgcgacctgt gatgccctgg
    11221 ttcaggcgca cggcgcgttg aaagggttgg ctgcgtcact gatgaaaatc gccaatgatg
    11281 tccgctggct ggcctctggc ccgcgctgcg gaattggtga aatctcaatc ccggaaaatg
    11341 agccgggcag ctcaatcatg ccggggaaag tgaacccaac ccagtgtgaa gcattaacca
    11401 tgctctgctg tcaggtaatg gggaacgacg tggcgatcaa catgggtggc gcttccggta
    11461 actttgaact gaacgtcttc cgtccgatgg tgatccataa tttcctgcaa tcggtgcgct
    11521 tgctggcaga tggcatggaa agttttaaca aacactgcgc agtgggtatt gaaccgaatc
    11581 gtgagcgaat caatcaatta ctcaatgaat cgctgatgct ggtgactgcg cttaacaccc
    11641 acattggtta tgacaaagcc gcggagatcg ccaaaaaagc gcataaagaa gggctgacct
    11701 taaaagctgc ggcccttgcg ctggggtatc ttagcgaagc cgagtttgac agctgggtac
    11761 ggccagaaca gatggtcggc agtatgaaag ccgggcgtta atctgcaaca tacaggtgca
    11821 gccgtggaat gatcaaacga agcggctgcg cctgaggttt ataacggtgc tgcacattgt
    11881 cggcatcgta atttaacaac tcaccaacgt ccggcacgcc cgcgccatta tcccgattaa
    11941 tcagtgcaat cagtggtgta gggcaggcgt gttggacttg tttttgatct cctttgtacc
    12001 agacgcgggc aatcggctgc accttcactg gacgtttgat ttttaacttc gcgttctgtg
    12061 gcagggcagc gatatcctga tactctcgct ccagttttct ttgccactcc tcgcgcgtcc
    12121 acggtgcgac actgcgtggt gatttcaggc ttttttccag ctgtgccagg acttcatcac
    12181 gatgcaaatt cttaatgata tgtttattag cccaaccaaa gcgtaaagtg gcggggtcgt
    12241 gcagaacggt gagcgtgcgg taagcattaa gggtgatcag ccccggcaaa tgacgatgca
    12301 cccattcaaa acgtgccgcg gtggggagtt ctgattcaac cgtgacgata tgctcgaacg
    12361 tggtcttgag tttattgatg tgctgaatat gactgaccaa cgctgcttgc gaaaggttat
    12421 cgacctggta acacaacacg ccaggcagac ggacagcggc cttgctgctg cgattttcgg
    12481 actgttgttg aataaataaa tggcggaaat gacgcaacgc cagcgactgc gcgtcgttgc
    12541 cgagatgttg ttttacctca atacgattaa gcggattatg ctcatcctct ttttttacct
    12601 ccggcaaaga gaacacgcgg gcaaccaata gcttgtgttg ctcaagatga gcggcaaatg
    12661 tagccagctc ttgttccatc tggcgaaagg tagtgttgag tcggtctacg agatcgtaac
    12721 gcgccataat attaaccact ttagttacaa catacttatt ttatagcaca gtcgtggtga
    12781 cttgaccatc gtgcgcgtag tgtcaggcag aggtaaattg cgggatgtta tgccataacg
    12841 gccagctaaa cgagaagcga gcaccaccca gttcgctgat gtcacaatta accgtaccgc
    12901 ccattgtcag tgctattgag tggacaattg ccagccccag cccgcagccg ccggttgagc
    12961 gatcccggct gggatcgagg cgaacaaaag gttcaaagat atgttcgcgg ttttctgggg
    13021 caatccctgg gccatcatcc tcaacaatta atgtcgctct attccccgac agtagcaggc
    13081 tggtttcaac cgttgaatgg cagtagcgca gggcgttatt gagcaaatta tccagcacgc
    13141 gctccattaa gcgcatatcc aacgccgcat aatggccttg catgagcgtt tttatccgta
    13201 ccgttttatc gggcgttact gcctgaatat ctgccagatg cgttgacagc cacaacggca
    13261 ggtctggttc gctaagatga agctcgtttt gtgggcgatc gagtcgggca taagtcagca
    13321 gctcttcaat taaagcttca agttgactga tatcacgatt caacgcctgg gattcggcgg
    13381 cgctcaggtt atcgctcatc tccagtcgat aacgcaggcg cactaacggt gttcgcagtt
    13441 cgtgagcgat accgtcaata agctgttttt tgctggcaat taaggcgttg atattgtccg
    13501 ccatctggtt aaatgcgata ccaagtcgtt caaagctcga accctcatca aagtggatac
    13561 gttcattgag atgcccatcg ccaaatcgtt gcgccgctgc ttccagtttt aacatatcct
    13621 gccagtgcgg acgcatccag ataaacaccg gaaaggcgag ggaaatagca ataaaagcga
    13681 tcagggcgat atccagcaat cgcatttgat ggaggtaata aagataagga acaggaccaa
    13741 ctgccagcac gtagtggctg cgcgggatac gctgcaaaaa cgtgtactga tcgtccaggg
    13801 cgacaatttc gccgccacgc agtcggtgca tggaaatatc atcaagatgg tatttactca
    13861 gtggctcgac acgcagatcg aaagagagat ttaaatccat ctctttcagc gtcttacccc
    13921 agtcgtgtgg ggggatctct cgcaattcgc tgcgcatcag atacagcgaa ctgttcatca
    13981 aatcatccag cgactgtttg cccgcgcgtt cggcggtaaa tttgtacacc agcccaacca
    14041 gcagagacat cacaaggaag cagacaaaca ataacaggta aaactggata aacaattttt
    14101 tcattggcat cgcccgcaac agaatactat tcgtattaat tcacgactgt ggagcaatgt
    14161 aaaccgccaa ctggataaga ccttgtttgg aagataatgt ccggaacaga cggatgtatc
    14221 cccggtgtat tcatattcag taaattcaat tttccaccgg ggcttgttta ttatgctaag
    14281 gtttaattta acgtgacgct ttgttgttct ttagtctctc tatccttaag aagacgggct
    14341 gatattacac cggacattat tgaaccattc acattaagcg ctgttcttgc catatcaacg
    14401 atcggctcta tggcaataaa aataactact aatgcgacag ggaaattaag tgttgaaagg
    14461 acaatcagcg ccgcgaatgt cccaccacta cctacgcctg ctacccctat tgagccaagc
    14521 gcgatggcag gtaaaagagt tgcgaggaat tgcaatgaga agggatcgat acccattgtt
    14581 ggagcaatca tcgccaccat cacggcagga taaatcccag cgcagccatt ttgtcccatg
    14641 ctggaaccaa aagacgctgc aatattagca atcgtatttg gaacccccaa tttttccaat
    14701 gcagaaatag ccagaggaat tgaagccgca ctactgcgag aaacaaacgc aaaggtaagt
    14761 acttgccaga cggtcttgta atagttaacg gggtttttac ctattaacag caaaatgccc
    14821 gagtgaacaa taaacatcac aaaaacggcc agataacagg cgacgatgaa cccgagcaaa
    14881 ttggtaaact gtgaaagctg atacgttgag aagactttcg ccattagtgc cattacccca
    14941 taaggcgtca gcgcaatgac aatacggacc attttcatta cccatatttg aatcgcgttg
    15001 atccccgcgc tcaatttttc gccctctgct ggtgcatctt tctttacttt taacagcgca
    15061 ataccagcaa gaattgtaaa gatgacgatg ccaataaccg aaacacttct tgccccggtc
    15121 agatcaagga agaggttggt tgggatcaat gacgtaatga gtcgcggtaa agataccgca
    15181 gctgttttcg agacgtcatc gctggttaaa gaggattgca tatgaccaaa ggcgctggca
    15241 tcaaggccaa ttgcactggc aatagccagg ccaactaaac ctgcaatcat gactaaacac
    15301 aacatacagg caacaatagt taaagacatc ttgccaatac cagcgctatt ttcgagctta
    15361 ttaatcgctg aaagaatcga aatgaatatg agtggaacgg caacaagttt taataaatta
    15421 acataaccgt taccagcaat attataccat tcgataacgg tttcactgct tgtctcagga
    15481 atgaaatgca gggagatacc aatgatagcc cccaggaaaa tcgcgataaa aacacttttg
    15541 gaaagggatt cgctcttttg atccgttttg aaaatccccc atgagcagat caaaaaaagc
    15601 acaatatatg tgaaagttgt aaccgttttt taagcctctt gcctcattca gcacctgaaa
    15661 tgccggaaag agtttgttgt aaaaaatctg cgccataaga cgcagggacg aggcgattat
    15721 agagaaattt tttctcattt tcatatcaaa tgtagggggt ttggaatttg aataagctct
    15781 tattaattga attcgttaat tgttctcttt ttcagatgaa caaatattag tcatctgatt
    15841 gtttattgtc actatcccat gcatgaggcg caaaaagata gcctttgtta cgcacagttt
    15901 taatgcgata aggttctgcg gcgttatcga gcagtttttt tcttaaccgc gaaatagcca
    15961 cgtccacgct acgatccagt ccgtcataac tgacgccgcg taaatttttc agcaatgcat
    16021 cgcggtccat gatttgcccg gcatgcgtag ctagttccca caataattcg aaatcagctg
    16081 tcgagagcga gatttcagtg ttagccaggg tgactacgcg gttgatgggg tcgatagtta
    16141 acgtgccgaa atgcagggct ttgtagggag taagagacgt ttcctgaaga cctttggtca
    16201 gtgtggcttg ctcattctga cgcaaatgca aacgtaaacg cgctagcaaa acagcagggg
    16261 gcgtcgtttt gagaatatag tcgcaggcac ccatttccag tgccaggatg tggttcatat
    16321 cgctatcgag agaggttaga agaacaatcg gtccagacca ctttgcgcgt aaatcacgac
    16381 aaatggtcat gccgtccttg cctggtagca tgatgtcgag taacaccaaa tccggatttt
    16441 ctcgcaaaat ggtttcttcg gcctggtcgc cgcgcggttc tacggtaacc tgcatatcat
    16501 gttttgccag gtacgcggca atcagtgaac cgacttccgc atcatcttcc acaaatacga
    16561 tagtgttcat aacattcacg gtagatataa aaacgtcaca atacaccgcg ctgtttttac
    16621 ttactatcaa tcgtttctaa acaagttttc atcaggtatt ctactgatga cttgttatgg
    16681 aagtgttaag gtaaaaagat ggggctggta atcaaagccg cgctcggtgc gctggtagtg
    16741 ctgttgattg gtgttttagc aaaaacgaaa aattattata tcgccgggct gattccactt
    16801 ttcccgacct ttgcgcttat cgcgcattat attgttgcca gcgaacgtgg cattgaagcc
    16861 ttacgcgcaa ccatcatttt tagtatgtgg tcgattattc cctattttgt ttacctggtg
    16921 tcgctgtggt atttcaccgg aatgatgcgc ttgcccgccg cttttgttgg ttctgttgcg
    16981 tgttggggga taagcgcatg ggtgttgatt atatgctgga taaagctgca ttaacgcaga
    17041 tgacgaccgc catcaagtgg gaaactgcgt ccggtgacaa agcaactggt aagtaagtaa
    17101 tcgaccaggt cgatcacttc tttctcgcca ggcgcggttt tcatcagtga tttattcagc
    17161 gcctgttgtc gatattcggc atcatcatgt tcattaaaca ggatcagcga tggcgcaata
    17221 gaattcactt tcacttccgg tgccagcttg cgggcaaacg agcgggtcat attatccagt
    17281 gccgctttgc ttgcagcata cgcaacatgt ttgtcgctac cgcgctccac cacataatcg
    17341 gtaaagtgaa tgatatcgct ggcggcgtgt ccgtgcccac gcagtaatct ttccagcgca
    17401 tggttgagca ggtatggggt attaacgtgg atctgcatca tgcaagccag tacgtcggcc
    17461 agtggcgcac ccggtttttc cgcaatccac gcactggcgt tatgcaaaat agcacgcaga
    17521 ccatgggtgg tttttagtac ttcatcggca aacgccatca caccgtcgtt ggtcgaaaaa
    17581 tcagcctgaa tacactgcgc acctgcatta atcagtccat caatggctgg atagtgtgtc
    17641 cgatagctga caatcaccgg ttgcttttga ttaatgaaat gccatgcgag ggcgaggccg
    17701 atgcgacgac ctccgccagt aattaatatt ggcaagggct gggttttacc catcgttatc
    17761 tcctttgcta tccaacgatg ggatgaacgt tatcccacca gcatccaggt cgccggaact
    17821 gaggcaatta acagcatgcc aatcagcacc atttcctgac ggttcagtac attatcatgt
    17881 gtatgcgttt tgcgcgcata caggaaaacc agcagccccg gtgcatacag aacaacggat
    17941 aacagcaagt gcattggacc agaggcgtac aataaccata agccataaat gcaggcaccg
    18001 acacctaccg ctttatgcag tggacgggtt gcgattttca gcaagaacgc gccgaccagg
    18061 aaatagggca ccagaatcat ttctgaggcg atggtcagca acgtgttgta atcggaaccg
    18121 gtcagccaga tgagtaccag acaaatttgc acgcagatgt tggtcaacca cagtgaggcg
    18181 gatggcgcag cttgtgcgtt ctggcgcgca aaaatgcgtg ggaatgcttt atgagtggca
    18241 gccaggaacg gaacttctgc cgccatgatg gtccagctta agtacgcgcc gcaaacggaa
    18301 acgatcaaac cggcagcgat gatgatttcg ccccatggtc ccatcatttc caccatcaga
    18361 ccggccattg acgggttacg aatttcagcc agttcaggac gtgccaccac acccagcgaa
    18421 agcagcgtta ccagcaagta aacgcccaga gcggagagaa ccgccagcag tgtcgctttg
    18481 ccaacatcac gtttattacg cgcacgcgca gaaacaacca cggcaccttc cacaccaatg
    18541 aaaacccaca gggtgatcag catggtgttt ttcacctgtt cccaaacagg tacgccaagt
    18601 gcaagtccgg tgaagtcgag cttgaaggta tccagtttga acatcatcat cgccagcaca
    18661 acaaacagac ccagcggcaa caattttgcc agtgtcgcca ccaggttaat gctggcagcg
    18721 gtttgcaccc cgcgcaggat caaaaagtga acaatccaca ataaagccga tgcaccgact
    18781 atcgattgcc aggtattacc atcgccaaac aggcgcaatt ccggcgtgtc cgtaaaaaag
    18841 cttaacgcgg aaaaaacgat caccagatag gagacgttgg cgatgaccgc gcacagccag
    18901 tatccccatg cggaacaaaa gccgattagc tcgccaaacc cttcgcgggc ataggtaaag
    18961 ataccgccat caagttcggg acgaatgcgc gtgaggatca gcatggcaaa ggccagcaat
    19021 aaaatgccag cgccagtaat accccagccg atgagcagtg ctgccgggct ggcaactgcc
    19081 gccatatttt gcggcagact gaaaacaccc gcgcccagca ttgagcttaa taccagcgcg
    19141 gtgagtgcgc tcagtcccag tttcttttcc atcgatatcc agtgattaaa acgtgataag
    19201 aagaataaat attagggcaa aacgcataaa aatggagttt gccactgaag cgcgggattt
    19261 tacgaagaga tgtgtctgca tgcaatggtc gcggggaaat ttgtgactaa aaatgcgaaa
    19321 ggcggcatca agccgcctta taggagtaac tgaatagtta tttatacaga tctgcgctga
    19381 cggtcaggtt attaccacgt ttctgccact ggcgggtgat gtggtaatac ttagcacctt
    19441 tcttcgcggc acgtttcgca acctgatagg agacttcggt catgttgccg tagttgccag
    19501 agaatttgat gctgtcgaac gggaccatca tcgctgctgt cgctttgttc agttcttcga
    19561 ctttagtgcc atccgggagc gtgacggtgt aacgcccgcc ttttgatgac tgggtttcaa
    19621 agaagcgacc gacttcagaa cttggtgatg cggtagtcgc aacacccggg atctcaactt
    19681 tcttcgcggc ttcgccaccg gcagccagag ctgcacgtcc tgcttcggaa tctgccggga
    19741 tcacatccgg gctctggacg atacgtttct tagcatcttt tttatagatg aatgcagtaa
    19801 tacgctggtt gccgccctgg ttggcatcga tttgacgaac gatgtagaaa gagtaggcac
    19861 ctttcgcttt tgccgctttg gtgatggcat cattgacttc tggctggcta cgatagaagc
    19921 cctggacggt gaccgtgtca aacggttcaa tcagaacagc ctgatctttc ggcagttcga
    19981 caacaccgtt aattacgcga ttacttgttt cttctgcttt ttcagcatcg gctttataga
    20041 ggtcagcgac cacacgccag ttaccgctgt taccaaaatc agaagtgtcg acaacataaa
    20101 aagaggcggc accttcttta tctgcgcgac gagaaacggc tttcaccgct tcgccaatag
    20161 cattaaaacg accggtaacc actacacggt caaaaggttt aaccgctgcc gcttgctccg
    20221 gtgtcagttc tgttgctgcg ttaacggaga atgccgtagc agaaagcagt gccgacgcca
    20281 ggagggtgtt cttaagcttc ataaaaataa tccttcgcct tgcgcaaacc aggtactggt
    20341 attgttatta acgagaaacg tggctgatta ttgcatttaa acggtgtaac tgtctgcgtc
    20401 atttttcata tcacattcct taaaccaatt ttaatcctgc tcaaatgacc gtctatgctt
    20461 aaaaaacagc cgtatcagca tcattactac tgaagcaact gaattgtata agttaattta
    20521 atgttaagta gtgattcgtg ccggggcgat gtctcgtttt acccgaccgt cgaagacaat
    20581 tatcagtctt tatccggcgt tctaaggtgt ttatcccact atcacggctg aatcgttaat
    20641 attttgcgag ttcacgccga aatactgatt tttggcgcta gatcacaggc ataattttca
    20701 gtacgttata gggcgtttgt tactaattta ttttaacgga gtaacattta gctcgtacat
    20761 gagcagcttg tgtggctcct gacacaggca aaccatcatc aataaaaccg atggaaggga
    20821 atatcatgcg aattggcata ccaagagaac ggttaaccaa tgaaacccgt gttgcagcaa
    20881 cgccaaaaac agtggaacag ctgctgaaac tgggttttac cgtcgcggta gagagcggcg
    20941 cgggtcaact ggcaagtttt gacgataaag cgtttgtgca agcgggcgct gaaattgtag
    21001 aagggaatag cgtctggcag tcagagatca ttctgaaggt caatgcgccg ttagatgatg
    21061 aaattgcgtt actgaatccc gggacaacgc tggtgagttt tatctggcct gcgcagaatc
    21121 cggaattaat gcaaaaactt gcggaacgta acgtgaccgt gatggcgatg gactctgtgc
    21181 cgcgtatctc acgcgcacaa tcgctggacg cactaagctc gatggcgaat atcgccggtt
    21241 atcgcgccat tgttgaagcg gcacatgaat ttgggcgctt ctttaccggg caaatcactg
    21301 cggccgggaa agtgccaccg gcaaaagtga tggtgattgg tgccggtgtt gcaggtttgg
    21361 ccgccattgg cgcagcaaac agtctcggcg cgattgtgcg tgcattcgac acccgcccgg
    21421 aagtgaaaga acaagttcaa agtatgggcg cggaattcct cgagctggat tttaaagagg
    21481 aagcgggcag cggcgatggc tatgccaaag tgatgtcgga cgcgttcatc aaagcggaaa
    21541 tggaactctt tgccgcccag gcaaaagagg tcgatatcat tgtcaccacc gcgcttattc
    21601 caggcaaacc agcgccgaag ctaattaccc gtgaaatggt tgactccatg aaggcgggca
    21661 gtgtgattgt cgacctggca gcccaaaacg gtggcaactg tgaatacatc gtgccgggtg
    21721 aaatcttcag tacggaaaat ggtgtcaaag tgattggtta taccgatctt ccgggccgtc
    21781 tgccgacgca atcctcacag ctttacggta ctaacctcgt taatctgctg aaactgttgt
    21841 gcaaagagaa agacggcaat atcactgttg attttgatga tgtggtgatt cgcggcgtga
    21901 ccgtgatccg tgcgggcgaa attacctggc cggcaccgcc gattcaggta tcagctcagc
    21961 cgcaggcggc acaaaaagcg gcaccggaag tgaaaactga ggaaaaatgt gcctgctcac
    22021 cgtggcgtaa atacgcgttg atggcgctgg caatcatcct gttcggttgg ctgtcaagcg
    22081 ttgcgccgaa agagtttctt gggcacttca ccgttttcgc gctggcctgc gttgtcggtt
    22141 attacgtggt gtggaatgta tcgcacgcgc tgcatacacc gttgatgtcg gttaccaacg
    22201 cgatttcagg gattattgtt gtcggagcac tgttgcagat tggccaggga ggctgggtta
    22261 gcttccttag ttttatcgcg gtgcttatag ccagcattaa tattttcggt ggcttcaccg
    22321 tgactcagcg catgctgaaa atgttccgca aaaattaagg ggtaacatat gtctggagga
    22381 ttagttacag ctgcatacat tgttgccgcg atcctgttta tcttcagtct ggccggtctt
    22441 tcgaaacatg aaacgtctcg ccagggtaac aacttcggta tcgccgggat ggcgattgcg
    22501 ttaatcgcaa ccatttttgg accggatacg ggtaatgttg gctggatctt gctggcgatg
    22561 gtcattggtg gggcaattgg tatccggctg gcgaagaaag ttgaaatgac cgaaatgcca
    22621 gaactggtgg cgatcctgca cagcttcgtg ggtctggcgg cagtgctggt tggctttaac
    22681 agttatctgc atcatgacgc gggaatggca ccgattctgg tcaatattca cctgacggaa
    22741 gtgttcctcg gtatcttcat cggggcggta acgttcacgg gctcggtggt ggcgttcggc
    22801 aaactgtgtg gcaagatttc gtctaaaccg ttgatgctgc caaaccgtca caaaatgaac
    22861 ctggcggctc tggtcgtttc cttcttgctg ctgattgtat ttgttcgcac ggacagcgtc
    22921 ggcctgcaag tgctggcatt gctgataatg accgccattg cgctggtatt cggctggcat
    22981 ttagtcgcct ccatcggtgg tgcagatatg ccggtggtgg tgtcgatgct gaactcgtac
    23041 tccggctggg cggctgcggc tgcgggcttt atgctcagca acgacctgct gattgtgacc
    23101 ggtgcgctgg tcggttcttc gggggctatc ctttcttaca ttatgtgtaa ggcgatgaac
    23161 cgttccttta tcagcgttat tgcgggtggt ttcggcaccg acggctcttc tactggcgat
    23221 gatcaggaag tgggtgagca ccgcgaaatc accgcagaag agacagcgga actgctgaaa
    23281 aactcccatt cagtgatcat tactccgggg tacggcatgg cagtcgcgca ggcgcaatat
    23341 cctgtcgctg aaattaccga gaaactccgc gctcgtggta ttaatgtgcg tttcggtatc
    23401 cacccggttg cggggcgttt gcctggacat atgaacgtat tgctggctga agcaaaagta
    23461 ccgtatgaca tcgtgctgga aatggacgag atcaacgatg actttgctga taccgatacc
    23521 gtactggtga ttggtgctaa cgatacggtt aacccggcgg cgcaggatga tccgaagagt
    23581 ccgattgctg gtatgcctgt gctggaagtg tggaaagcgc agaacgtgat tgtctttaaa
    23641 cgttcgatga acactggcta tgctggtgtg caaaacccgc tgttcttcaa ggaaaacacc
    23701 cacatgctgt ttggtgacgc caaagccagc gtggatgcaa tcctgaaagc tctgtaaccc
    23761 tgacggcctc tgcggaggcc gtcactcttt attgagatcg cttaacagaa cggcgatgct
    23821 ttgacctccc gctgtttgtt caagcgcaat tttgacaata attgtcaacg gcacggaaag
    23881 cagcataccc accggtccta acaaccatcc ccaaaaaatc aacgacaaaa ataccaccaa
    23941 tgtcgaaagc cccagcccac gtcccatgat gcgcggctcc agaatattgc cgaagaccag
    24001 attaatcagc agatatcccg ccagcaccag caacgcttcg tagaagccat taaacaccag
    24061 tacctgagcg atagggggga ttgccgcgag aactgaacca atattaggga tgtaattaag
    24121 cgcaaaggcc agcaatcccc agacaaaagc gaagcgaaca tcgagtgcgg cgagcatcgc
    24181 ccaggcgacc aggccggtga tgatgctgat ggctgttttc agcaccagat aatgagaaac
    24241 actgtcaatc gcacgttgaa tcgccgccat cccttcaacc ggacgcgcca tcatttgctg
    24301 aaattttccg ggcaattgtg gcacttcgag cagcataaac agcaccgtca gcagcaataa
    24361 aaatattgat gacatggcat tagataactg cgtcaataag ttggtgagca acgtcatcgc
    24421 cgcgttcgga tcaatgtaat gcgccagttg gtcaactgag acgtcaatcc ctacgcgttg
    24481 caacaacggc tcaagagctt gcagcggcgt cataatagag ttgcgatatt gcggtaacgt
    24541 ccgcgtcaac tcgttgagcg cggaacccag ataagctaat agcaacacca tcgccatcac
    24601 gatgattgtc atcaaaatcg acactgccag tacacgcggc acacgccagc ggaccatatg
    24661 ttgcaccagc gggttaagaa taacagcaat aaataatgcg agaataaacg gcacgatgat
    24721 ctcggcggca aaacggatac cgcagagaat aatgaccagc attcccaaca taatgacgat
    24781 ttttaggcca ttgagcgtga tgatcggctt tgccatgctt gtccccgttt ttcttccttt
    24841 gctaataata atgtcttttt cgctccgtca tcataacgaa aatcaaaccg ctcaggtaaa
    24901 gaagtgaaaa tattttgagt taattcttaa gctatgatac aaatcaggcg tgttcaacta
    24961 ccgaggacaa ttatcatctg cgatgacgag aagcaacact gcggataatt gtaatattat
    25021 ggacaatttg ttcaggacgt tattttctac atttacccat cttcgtacct catccactat
    25081 cttgctcgta ggtgagcagc actggcgcaa cgcgctttag tcctttcttc tgcctaagct
    25141 tgccctatgc gcagggcttg accactttac tttggtttcg ctgaattaag cgaaaattaa
    25201 aatagttctc ttgcaggaga aggacaatgt atatttattg gattttatta ggtctggcta
    25261 ttgctacaga aattaccggt acgctgtcaa tgaaatgggc gagcgtcagt gagggaaatg
    25321 gcggctttat tttaatgctg gtgatgattt ctctgtcgta tatatttctc tctttcgccg
    25381 ttaaaaaaat cgccttaggc gtagcttatg cgctgtggga aggtatcggt attttattta
    25441 ttaccttgtt tagcgttttg ttattcgacg aaagtttatc gctgatgaaa attgccgggt
    25501 taaccaccct ggtcgccggg atagtgttga tcaaatcagg tacccgtaaa gcgcgtaaac
    25561 ctgaactgga ggtgaaccat ggcgcagttt gaatgggttc acgccgcctg gctggcattg
    25621 gcaatcgtgc tggaaatcgt tgctaacgtc tttttgaaat tttctgacgg ctttcgtcgc
    25681 aaaatatttg gcttgctctc cctggcggcg gtgctggcgg cctttagtgc gctttctcag
    25741 gccgttaaag ggatcgactt gtctgtcgtt tatgcattgt ggggcgggtt tggtattgcc
    25801 gccacgttag ccgcaggttg gatcttgttt ggtcaacggt taaatcgtaa aggctggatt
    25861 ggcctggtct tgctgttggc tggaatgatc atggtgaaac ttgcctgatg aagacgctgc
    25921 ccgcgctgtc gggcagcgtt tgaacattat ttttgcgaca gttgatccag cttgtcgcga
    25981 aaaccggtaa cagaaatggc ccggttatcg gcgcgccagc gatctttcgc ggcaggagcc
    26041 gaactttgca ccccaattaa ttgccagccg tcatcggtat gcaacatcag aggcgaaccg
    26101 ctgtcacccg gcaaggtatc gcactgatgt gacatcaccg acgtttgcgc ccagccagtc
    26161 acttcacagt tttgatgact gtataacgta tcgagatgat cttcagggta tcccgcctga
    26221 gtcactttac gaccagccga ttttaatgcg gcagtaagcg cggctttatc tccctcaaat
    26281 aacggcaacg gtgtaatgcc agaaggggga ttacgtagca caatcaatcc gaagtcccac
    26341 ggtgcagctg cgggaggaac aatccaacca tccccatctg cttttaaccg ctttcccagt
    26401 gtcggatcaa cgcggccttc tatgtcgtgg atctcatagc gccaaagacc tttatttgac
    26461 acaaaacgca gcgctaccgc cttatccgct ttacccttag gcggcgtcaa taaacagtgt
    26521 cctgccgtta atgccagatt gggtgcaatc agcgtcgccg tacataaatt gccgctggcc
    26581 gtttccagtt gcccaaccgc atcccacggt gattgggtcg tgtcattcac tggcacacga
    26641 tcatcatgac caaaaaacag ggtgctgacc tcatcgtttg ccgatttggc aacgtctggt
    26701 ttatctgcaa acacaaaagc agacgtcaaa ctaattgcac ccaacactac agcaatggtt
    26761 gtacgcatat cacactctgg tgggtaatta tgattattaa aagcaaaccc tcataaatac
    26821 tatagacggg acgggatgaa agtgggagta aaatcagata actacagtca ggaaagataa
    26881 aaacgggcgg caatcagtgc gcataaaata aggatgataa gaatgaactc aaagcgataa
    26941 cggcccacca tactaccctc cgctaaaggc ggtgctaaac gttacccgct tagcaccgta
    27001 attttcaggc gcgagggggc gcgccagcat tactgttgaa aacttacgct gcgggttgtg
    27061 cagcaggttt tgccggttgc tgatggccgt gtttcccggc gtgtttcttc gctgcctgcg
    27121 ctttttgttc aggggctttc tgttcagctt tcgtattttt atgatgcttt ttagccgcct
    27181 gcgctttctg ggcaggtgct gctttatgct gttttttatg atgtgtagtt ttcgctggcg
    27241 ctgctttggt ggtcgtcgca gtcggagcag gtgtggtcgc agtctctgca gcaaaggcgg
    27301 cagaagacag acccatagca gcggcaacaa ccagagctaa tacttttttc attgtcatac
    27361 cctcaatttg ttttttcatt taaccccact gcggggccgt tgaaataact atatccctgg
    27421 gaatggcaga cttccgtgag tggttggttt cagcgtgtaa cgatatgtac aaacgctgaa
    27481 taaattacag cgttgataat aacgtattgt gacttaagga aaatttagct accaataata
    27541 gtagtcttga tcgggttaac tattatacta cttacaaagc ggtagaataa ctgcgcatca
    27601 gtaataaatg acacacagca aaatgaatcc gtttatttgg gtacttataa tcctgatgac
    27661 gctagatgcg ctgcgggaat tggctggcgc ttcgtctatt ttaggatggc tattaacgtt
    27721 ggtttgagca ggcaataagt ccggacgggt atttaccgca gtccggactt atttttcagg
    27781 cgtgcagacg acgatgcaaa cgcgtcccga ccagcagagc aatgaccagc atcagagcaa
    27841 taaatgcgcc gacgccgttc cagccatagt tatgccagaa aacaccaccc agcgtcccgg
    27901 caatactcga ccccagatag taactgaaca gatacagcga ggaggcctgg cctttagcgc
    27961 gttttgcgcg ggggccgatc cagctgctgg ctactgagtg ggctgcgaag aatccggctg
    28021 agaagagtaa cattccggca aagatcagcc acagcgagct aaataaggtc atcagtaaac
    28081 caaacggcat aactcccgtc gaaaacaaca tcaccggacc gcgcccatag cgggtggtca
    28141 tggttccggc tttgggtgag ctccatgtac cggtcaaata agccagcgat aataagccaa
    28201 ccacggcctg actgacatgc tagggggaaa gcatcaaccg atagccgatg taattaaaca
    28261 gcgtgacgaa cgaccccatc agcaaaaagc cttctgcgaa caataacggt aatccccggt
    28321 cacgccagtg cagacgaaag ttgataaata acgtcttagg gcgcagcgaa gtcgggcaaa
    28381 aatggcgtga ttcagggagg attttccaga acatcaacgc cgaggccagc gcgaaacaac
    28441 cgattgccgc cagagcaatt cgccagttga aaaagtccgt gaagacacca ctaattaagc
    28501 gtccgctcat gccgccaatt gagttgccgc tgatatacaa ccccatcgaa aaggccacga
    28561 aactggggtg gatttcctcg ctaagataag tcatgccaac agctgccacg ccacttaacg
    28621 aaagcccaat caaggcgcgc ataatcaaaa tgccgtgcca gctggtcatc attgtcgaaa
    28681 gtaacgtaca aatggaggcc aacagtagcg ccgtgaccat cactggtttg cgaccaatgg
    28741 catcggatag cgggccagta aatagcaaac cgatagccaa catcgccgtg gaaatggaca
    28801 gtgaaatact actgttcgcg ggcgttaagc caaactcctg cgaaagcacc ggaaggatag
    28861 gctgcacaca atagagaagt gcaaaggttg ccagtccggc agagaacagc gccagggtga
    28921 cgcgcataaa ttgcggcgta ccgcgtttaa taaattgatt tggctgagaa atgctttgct
    28981 tgtcagtgtc gcttgccgga gcgccatcaa cagttgtagt acggctcact tgaaatcctt
    29041 gctaaatatg cctgtggatc aggcttatac atagggtagg aaaatcgaat tgttctgtct
    29101 aatatattaa taatctcaaa taagatgttt taaatatgaa tattgaactt cgtcatctgc
    29161 gttactttgt tgctgttgcg gaagagctgc attttgggcg cgcggctgcc cgcctgaata
    29221 tttcgcaacc gccgctaagt cagcagattc aggcactgga gcaacaaatc ggtgcccgac
    29281 tgctggcacg aactaatcgc agtgtattgc tgacggcagc aggaaaacag tttcttgcag
    29341 atagtcggca aatcctgtct atggtggatg acgctgccgc tcgcgctgaa agactgcatc
    29401 agggtgaagc gggggagttg cgtattggtt ttacttcgtc cgctcctttt attcgggcgg
    29461 tgtccgatac gttatcgctg tttcgccgtg attatcctga tgtccattta caaacccgcg
    29521 aaatgaacac tcgcgagcaa atcgctccgc tcattgaagg aacgctggat atgggattgc
    29581 tgcgtaacac agcgttaccg gagacgcttg aacacgcagt catcgtccat gaaccgctta
    29641 tggcgatgat cccgtacgat catcccctgg caaataatcc gagtgtaaca ctggctgaac
    29701 tggctgaact ggctgaactg gctgaactgg cgaaagaacc ctttgtcttt tttgatccgc
    29761 acgtcgggac tgggctgtat gacgatattc tcggactgat gcgacgttac catttgacgc
    29821 ccgtcatcac tcaggaggtg ggcgaggcaa tgaccatcat cggtctggtt tccgccggtc
    29881 tgggtgtttc aattttgcct gcgtcattta aacgtgttca gctcaacgaa atgcgctggg
    29941 tgccgattgc tgaagaggat gcggtttctg aaatgtggtt ggtctggccg aaacatcatg
    30001 aacaaagtcc agctgcgcgt aactttcgta ttcatctgct gaatgctctc aggtgaggga
    30061 aatttcagcg aaaaagcccg aaaaatgtgc tgttaatcac atgcctaagt aaaaatttga
    30121 cgacacgtat tgaagtgctt caccatagcc tacagattat ttcggagcgc gaaaatatag
    30181 ggagtatgcg gtggttgctg aaaaccagcc tgggcacatt gatcaaataa agcagaccaa
    30241 cgcgggcgcg gtttatcgcc tgattgatca gcttggtcca gtctcgcgta tcgatctttc
    30301 ccgtctggcg cagctggctc ctgccagtat cactaaaatt gtccgtgaga tgctcgaagc
    30361 acacctggtg cgagagctgg aaatcaaaga agcggggaac cgtggccgtc cggcggtggg
    30421 gctggtggtt gaaactgaag cctggcacta tctttctctg cgcattagtc gcggggagat
    30481 tttccttgct ctgcgcgatc tgagcagcaa actggtggtg gaagagtcgc aggaactggc
    30541 gttaaaagat gacttgccat tgctggatcg tatcatttcc catatcgatc agttttttat
    30601 ccgccaccag aaaaaacttg agcgtctaac ttcgattgcc ataaccctgc cgggaattat
    30661 tgatacggaa aatggtattg tacatcgcat gccgttctac gaggatgtaa aagagatgcc
    30721 gctcggcgag gcgctggagc agcataccgg cgtaccggtt tatattcagc atgatatcag
    30781 cgcatggacg atggcagagg ccttgtttgg tgcctcacgc ggggcgcgcg atgtgattca
    30841 ggtggttatc gatcacaacg tgggggtggg cgtcattacc gatggtcatc tgctacacgc
    30901 aggcagcagt agtctcgtgg aaataggcca cacacaggtc gacccatatg gtaaacgctg
    30961 ttattgcggg aaccacggct gcctcgaaac catcgccagt gtggacagta ttcttgagct
    31021 ggcacagctg cgtctcaatc aatccatgag ctcgatgtta catgggcaac cgttaaccgt
    31081 ggactcattg tgtcaggcgg cattgcgcgg cgatctactg gcaaaagaca tcattatcgg
    31141 ggtgggcgcg catgtagggc gcattcttgc catcatggtg aatttattta acccacaaaa
    31201 aatactgatt ggctcaccgt taagtaaagc ggcagatatc ctcttcccgg tcatctcaga
    31261 cagcatccgt cagcaggccc ttcctgcgta tagtcagcac attagcgttg agagtactca
    31321 gttttctaac cagggcacga tggcaggcgc tgcactggta aaagacgcga tgtataacgg
    31381 ttctttgttg attcgtctgt tgcagggtta acatttttta actgttctac caaaatttgc
    31441 gctatctcaa tttgggccag gaaagcataa cttagacttt caaggttaat tattttccta
    31501 gtttatattt gtgaagcata acggtggagt tagtgatgct gaagcgtttc tttattaccg
    31561 gtacagacac ttctgtaggg aaaacggtgg tttcccgcgc attgctacaa gcgttagcct
    31621 cccagggaaa aagggttgcg ggatataaac ccgtagcgaa ggggagcaaa gagacacccg
    31681 aagggctgcg taataaagat gccttggtgt tgcagagtgt ttcaaccatc gaactgcctt
    31741 atgaagcagt taatcctatc gcgttaagcg aagaagaaag tagcgtggcg cacagttgcc
    31801 caatcaatta caccctcatt tcaaacggcc tggcaaacct gaccgataaa gtcgatcatg
    31861 tcgtggtaga agggactggc ggctggcgca gtctgatgaa tgatttgcgt ccactctctg
    31921 aatgggtagt gcaggaacaa ctgccggtgt tgatggttgt cggtattcag gaaggttgca
    31981 ttaaccatgc actgctaaca gctcaggcga tcgccaacga cgggctgccg ctcattggct
    32041 gggtggctaa ccgaatcaac ccaggactgg cgcattatgc ggaaatcatt gatgtgctgg
    32101 gtaaaaaact tccggcaccg ctcattggtg aactgcctta tctgccgcgc gccgaacagc
    32161 gtgaactggg gcaatacatc cgcttagcta tgctgcgcag tgtgctggcg gtagatagag
    32221 tcacggtgta acgtctgcga aattaccgac gcaattacgc aggcaatcaa taaaccgggg
    32281 agtagctgat actccccggt catttcacat atcatcaacg tcgacataat cggcgcgtgc
    32341 gtggttgccg ccaacagtgt cgccatcccg gtcaatccga gtaaaagcgt aatttcttcg
    32401 ccatcaggga accataatcc caggctacga ccatacaaca tgccaatggc aagaccgata
    32461 aatagcgtcg gtgtaaagac accaccgggt gcgccggaac cgctactcgc cagcacggca
    32521 cacagtttac aaaggaagat cccggcaatg atcattaaca gtggtggggc ggttaaaaag
    32581 gattgtacgg tgctatagcc gttgccccac actgcaggtg tgaacaggga aagcagaccc
    32641 acgatcaacc cgcctagtgc cagttgccag ggcggcgcaa gtttgagact cacaaatcca
    32701 cgatgacagg cgttcattaa cgttaacaac agtggtccgc acagacctgc cagcacacct
    32761 gtactgataa tcagcgcata gtcacgagcc tgaaccgtca ctgagagttg tacgttgtag
    32821 agtaacgcgt cgctatgatt aatcagattg ctaaccagcc atgccacgat ggcggaaata
    32881 atcaccgggc cgagagaggc caacatcata gtgccaaaca gcacttcggc tataaataaa
    32941 ctgccagcaa gcggggcacg ataggccgca gccattcccg ccgcggcccc acaggcgatc
    33001 cataatttcc attcctggcg tggcgtaaaa cgttgggcaa aacaggaggc ggcaagggca
    33061 gctaaaagaa tcatcgcacc ttcgcgacca attgcactgc cgctggttac taccagcagc
    33121 gaggcaagcg atttaaccag gcttgctgcg taatcgaact gtccatcggt ttgcaacgct
    33181 tccatgtaat cggtcggcgc atgagggcgt tgttgggtaa atttctgcca gcccatcagc
    33241 aacaaacccg ccgccagtcc gccgagcgcc ggagttagca gccgtcgcca ggaggaaagg
    33301 tttgtcgctg cattgaccag actgccggag tcattattga ggaacaacca ctccagtagc
    33361 agcatcgcat gacgaaaccc ggcaacggca aagacagcga gaataccgac gactgttgcg
    33421 ataagcagac ggcggaacat agtgcgcaga tccggataga tgtgtagatg atgcatgagc
    33481 agaaatgggc aatgaaacac gggcgactat tttgcggcaa aagaggccaa ccgcaaaagc
    33541 agtgcgtgct ttaagagata aaaaaacgtg acattgtaac gtattattgc cgaatcgcgc
    33601 gtaatcgtca ccatccggca atattacggt gatcctatcg aaagagtggt ttaaccgcga
    33661 caggaatcaa cagttgtgat tgccattgcg ccagcgttaa gcgagccagt tcacccagtg
    33721 cacggtagaa agggtgttct gctttttcga taaaaacatc aagaaaacgt gttgaccacg
    33781 gaaaaaggtg ccatgccagc aattcttcgc attctgtctg gcgaccattc tccgccaacc
    33841 acgcagccat caacagcagt gaaccaaaat gatcttccgg ttcgttttgc ttcatttcaa
    33901 actgaatgcc tttctcgcgc atccactgac gaagtgccaa tgttgaatcg ccaaacagca
    33961 cagattcgcg atccagccag accgaccccc acggcggaga cggcagtgcc cacgggccga
    34021 caaacaaacg ctgccaggcc tgggcgtgag tctcttcaca ctgtgtctga aaagccgtca
    34081 ccagcggcgc taatgacgct tctggtagag gccactgagt ttcccagccg tcactggtga
    34141 gtaccgcaac cagaggtgcg gcttccgcac tctctggagc gtaataaaac agcgcgccca
    34201 acacgcgcgc cgcgacagaa aaattatctt gctgtgaaaa atgggtcatt cctaacatcc
    34261 tgaaatgtgc gggttgcccc gcaccaattt gttaacctgc aatagccatt cctactgtca
    34321 tgtgcaggcc ataaaagagc acacgcccga tcatctcacc aacaagtatg aggattagcc
    34381 ccagaacaag tccggcaacg tgaggttcac ggcgacgaat gagcggacaa agccagcatc
    34441 caagacctgc gcataacaat accacacgcc agacctgtaa ggaggcgtaa tctggtacca
    34501 gtgcgctggc ttgttgcacg gatgagtgaa tagacgccag agacaaacct tgcagcacaa
    34561 tcacctcggc acaagcgatc agcgccagaa cgctaatgat ggcaaatggt gtggtattaa
    34621 atgtaacgcg ggctgcccgc agaattgcgg cagcaagtat tggaccgctc aacaataccg
    34681 tcaggaagaa agccagcgta gtgtaaccgt tatgccaggt tggcacggtg tcgatttgat
    34741 acacgcaggt catcatccag acgaaaatga cgccgagcgc catgctgaca agtaaccaga
    34801 gtttccccaa cgcttgcggc attttaccga tgaccgccac cagccaccat agtccgccaa
    34861 cggcaaaaaa tatggaaccg gcagcaatct cgttactcag accagaagct ccgattcgat
    34921 tcagcgagtt aaacgctcgc agtggcgatc ccaggtgcat gacagaggca ataaacccga
    34981 tgcccatcaa cagccagagg aaaaacatgc cacgaacaat gtgctgacgg tctgcgtcat
    35041 ttttggcggc gaaccagcca attccgctaa caatcagcgc gcctactacg cattgaccca
    35101 atacggtaaa gataaccagt ggccattcat gccatccatt tcccatttta cacttcctcc
    35161 ggattagcca gataaccagt ggtatcgccg gtcgggcggc tgttggcgtt aggtttgata
    35221 acgatattgg gttttgtgaa atgcgcgcgc ggcagtggcg cgacggcagc cagagtgccg
    35281 tgcttctggc gcagctcttc aattggaccg aactcaagtg cacgcagcgg gcaggattcg
    35341 acgcatatgg gttgtttccc ctcggcgacg cgcgaataac aaccatcgca cttcgtcatg
    35401 tgcccttttt cagcattgta ctgtggtgcg ccgtacggac aagccatgtg gcagtagcgg
    35461 cagccgatac aaacatcttc atcaaccacc acaaagccat cttcacgctt atgcattgcg
    35521 ccgctggggc agacttttgt acatgcgggg tcatcgcaat ggttacagga gatggagaga
    35581 taataagcga acacgttctg gtgccagatg ccattatctt cctgccagtc gccgccagca
    35641 tattcataaa tacggcggaa actgacttcc gggccaagat ctttaaaatc tttgcacgcc
    35701 agttcgcagg ttttacagcc agtacagcgg ctggaatcga taaaaaatcc atattgtgtg
    35761 gtcatgggct actccttaaa ccttttcgat ctggacaaga ttgctgtgcg acgggtttcc
    35821 ctttgccagc ggtgaagggc ggtgagaggt cagaatattg atactgccgc cgtgatcgac
    35881 ccggtcacca aacatatccg ctttaagcca cgcaccttgc ccgatggcgg taacgccagg
    35941 cagaatacgc ggagtcactt ttgcggcaat cagcatttct ccattattgt taaatacccg
    36001 cacggtatcg ccatgacgga taccgcgtgc ctgagcatca atggggttga tccacacctc
    36061 ttgtgggcag gcctgctgta acacatcaat attgccgtag ctggagtggg tacgcgcttt
    36121 ggtttggcga cttcactcag catccagtag atgggtttgc gttcaaattt tgctgaggtt
    36181 gcgggttggg cgaggatcac atagcccata ttccctgcag attcatgaga gataaggtct
    36241 tcttgctctg ttggcatcag gtcaggcaac aggatatcgc aatacttagc cgaggccgtc
    36301 atgaagtggt caatgccaac aatcatctcg cacttgctgt catcctgaag cacctcatgg
    36361 gtgtgattga tgtcgccatg ttgattgatc aatgtgttac tggcgtagca ccataaaaac
    36421 ttgatgggga catccagttt ttcttttcca cgaacaccat cacgggtcgc ggtcattttc
    36481 gtaccatggt cgatggcatc tgtccatgta aaaacggaaa tctgcgtttt aacaggattc
    36541 tcgagcatcg ggaaccattc tacccccaga tcccagctac cttcgcgtac acctgagttg
    36601 ccgccgttta tgccgacgtt accggtaaga acggaaagca tggcaatagc gcgggacgtt
    36661 tgttcgccgt tggagtgtcg ttgtggcccc caaccctgac aaatataagc aggttttgct
    36721 gaaccgatct ctcgtgccaa ctggataatt ttttctgccg ggatgctggt gatttttgct
    36781 gcccattccg gcgttttagc tatgccgtca ggcccttcgc ccagaatata ggctttataa
    36841 tgcgcgttac gtggtgcgtt ggcgggcagc gttttttcat cgtaaccaac acaatatttg
    36901 tcgagaaatg gctgatcgac catgttttca gtaatcagta cccaggcaat cgcacaggcc
    36961 agtgcgccat cggtgccagg gcgaatgggc agccattcat cttcacgccc ggcagctgtg
    37021 tcgttatagc gtggatcgat gacgatcatg cgtgcgtttg aacgttcgcg ggcttgctcg
    37081 acgtagtaag tgacaccacc gccgctcatc cgcgtttctg ccgggttatt tccgaacata
    37141 acgaccagtt tcgtattggc gatatcatcc gggctgttgc catcattggc accgaacata
    37201 taactcattg cggcactgat ctgtgcggta ctgtagctgc catagcgact gagaaaaccc
    37261 ccgcaagagt tcatcagacg gtacgggacg ttcgagttgg tgatatttcc gccatctacg
    37321 cctgttccgt acaggacatg tacagcctca ttgccgtaat ctttcaggat ccgccgaaga
    37381 ttatcactga tggtatccag ggcttcgtcc caacttatcc gttcaaattt accttcaccg
    37441 cgctcgccga cgcgcttcat gggatatttc aacctgtcag gatgattcat ccgtcggcgg
    37501 atagagcgcc cgcgtaaaca tgcccgaacc tgatgattac cgtaaacgtc gtcacctgtc
    37561 gtatcagact ccacccagta cacggtgtca tctttcacat gcaaacgtag cagacagcgg
    37621 ctcccgcagt taacggtgca ggaactccag accgttttct cttctaccgg agcctctgcc
    37681 gcccggacca tttgggaaaa tggcagagtg aaagcactgc ttgccagcgc gagactgcca
    37741 agtgcggagg ttttcatcag acttctatgg ctgatttcag ccttcatgag cgcctctgtg
    37801 gtatggattt tcatcattac tcacttattg cttttcaaac aaaatgtcat gccagaattt
    37861 atggttgtag tgggttatat tttttcgatc tcgaccagat tagtgtgctg cgggtttccc
    37921 ttcgccagtg gtgaagggcg cagagtggtt agcgtattca cacatccgcc atggtcgatt
    37981 ttatcgccag acatattggg ctcgtgccag gctccctggc ccatagcgct aactccaggg
    38041 agaatgcgtg gtgttacttt ggctggtagc cgaacttcgc cacgatggtt aaacacccgc
    38101 accatatcgc cgttggcaat cccacgtttc tgtgcatcta tagggttgat ccacacctcc
    38161 tgacggcagg ctgccttcag gacatcaatg ttgccgtagg tcgagtgagt acgggatttg
    38221 taatggaaac caaacagttg cagtgggaag gttctacgtt caggggagtc ccagccttca
    38281 aaggttgagg cataaactgg cagtgggctt atcacttcat ctttttccag ttcccaggta
    38341 cgggcaattt ccgccagctt gctggaataa atttcaatct taccggaagg cgttttaagt
    38401 ggatttgcct cggggtcgtc acgaaatgct ttgtaggcga caaaatggcc attgggatct
    38461 ttacgcttat agatacccat ttttttcagt tcgtcgtaag acggtaacgc cggatctttg
    38521 gcaagcattt tggcgtacag atgttgtaac cattgttcct gcgtgcgacc ttctgtgaac
    38581 ttttgataga cgtcaggtcc aagacgtttc gcgacttcac tcaggatcca gtaaatcggt
    38641 ttacgttcga atttttcgct ggtgactggc tggaggaaaa tgagatatcc catgttaccg
    38701 gcgtagtcgt taggaataat atcttcctgc tcaacggtca tcaggtcttc cagcagaatg
    38761 tcggcatatt ttgccgatga ggtcataaag ttttcgatga ccacaatcat ttcgcatttc
    38821 gattcgtcct gcagaatttc atgcgttttg ttgatgtcag aatgctgatt aacgagggta
    38881 tttcccgcgt agttccagat ggactccact gtcagacgga tggacggact tgaagtagtt
    38941 gactcaacgc tacttaaggt cctgatatga ctgacattaa ccatgacaac actgcatatc
    39001 catccagtta tttctctggg tctccctatc tgtatgggca agtctacacc ccaagtcctg
    39061 acacaaggct gacacaaatc aatctactcg gcgtagctaa gcctcagtta gtccgtagag
    39121 ccaatggtcg atatactatc cgcttcagac tcaagggaca gaccacaccg tttctttcag
    39181 tatcgacccg cagcacagac aggagagtag ccacaatgcg tcagagagag cttgcagcca
    39241 cagctaaggc cttcatgttg gatagacctg aagtctcact acaggagctt acagagcatc
    39301 tccggtctat ggctgagcag ttcctgacag acgctagtga cgattactgg aatggacttg
    39361 aggtcgctac tctggtcgac gagaagtcca acctgaagga gctagccgct acgcaagcct
    39421 tgagtctgga ccagcagaag ggcataaggt tagcgctgga agtcctgacc gcagcacagc
    39481 agagagtcga cactggtgat acctctggtc tgattaagct gattgatgat aataaccata
    39541 ctgattactc aactataggc gactctacgt caattctgaa gaatgagcag ggggatagac
    39601 cagtagtttt cacacaggag cgccaacctg aggtcactat ctgtagtttt tctgagctag
    39661 tgtcctcgct gctggctgag aaggtccaga ccctgaagtc ctccagctac aaggacctct
    39721 cgtcctcact aaatactgtc tcccggttcc tgcctgaaga catggacctg atgtctcgct
    39781 ctgagtggct ggctgtcagg gacgctatgc tggcttctga ggtgagaccg tcgacgatta
    39841 acaagctgct gactaaggcg aagatgtgtc tggactatgg cctgatgaat gggcaacttg
    39901 agggccgcaa cccgattgag cgcatgaagt taactaagga tgtagactcc aagcgtagag
    39961 cattcactga cgaggagatg gagagacttc tggtccgtgt tgagtctgag tatcagttca
    40021 cacgacacac ggctcacacg acctctgagg cccgtagatg ggcgtctctg gtgtctgtag
    40081 ttactggcgc tcggtctgct gaggtctgcc acctgactaa acgtgacatt gtaaccttgg
    40141 acaatgggct ggtctgtatc gacatcaatg aagatgggga cggtaagtct gtgaagaaca
    40201 agcattcagt ccgacttgtg ccgttgaccg atggggcctg tggtcttgac ctgaagtcct
    40261 tcctcgaatg ggtagacatg cagcctgacg agggtcctct cttcgggatg actccaagcg
    40321 catactctag ctggtttaac tcaagggtcc tgactgaagc tctgggagac gctacggatg
    40381 tctctctcca cagtctgagg cactggctag cgaccagaat gaaagagcgt ggagccaatc
    40441 tggtggacgc tcagggcatc ttaggtcaca gcagtcagtc cattacgtat gacctgtacg
    40501 ggaaaggaca cgctgttggt aggctggctg aggtgctgaa gacagcttta ctttaagtag
    40561 gaagacccaa ggtgggagac catatacggc ttgatggttg agaccttggg tcactgaagt
    40621 ctgactgaag ttagggaggg tgataataat cataatccct cctgactata ggcccactgg
    40681 ttcgccttcg gctgtccgac tctaagtctg tctgtggtct tatttaaact ggttccctaa
    40741 ctcaggtcct ccaacttggt gtcttactta aactggttcc ctaactcagg cccctccaaa
    40801 tttcgttgtc tgtcagcgac ttaaatatcc cctcacctta gaagagttta gggttcagac
    40861 ccttaactat caccttagga gagacgggcc gtcctccacc caatcaaaac cttaagagga
    40921 ggaccataca tggctattaa aattcagtgc tacaccgagt ccgacaatgc ggctttcttc
    40981 ggtgacgatt ccgaccacca gcaccagcta ccagcaccga catatgacga acctaaggcc
    41041 cgcacaggta a
//
LOCUS       DAEBXN010000028        40544 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.028, whole genome shotgun sequence.
ACCESSION   DAEBXN010000028 DAEBXN010000000
VERSION     DAEBXN010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40544
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.028"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(<1..956)
                     /locus_tag="KJL99_001490"
     CDS             complement(<1..956)
                     /locus_tag="KJL99_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HBD4436268.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKIEVNLGAGEAGS"
     gene            1202..1828
                     /gene="gstB"
                     /locus_tag="KJL99_001491"
     CDS             1202..1828
                     /gene="gstB"
                     /locus_tag="KJL99_001491"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HBD4436269.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(1825..2939)
                     /gene="yliI"
                     /locus_tag="KJL99_001492"
                     /pseudo
     CDS             complement(1825..2939)
                     /gene="yliI"
                     /locus_tag="KJL99_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
     gene            complement(3050..3433)
                     /gene="bssR"
                     /locus_tag="KJL99_001493"
     CDS             complement(3050..3433)
                     /gene="bssR"
                     /locus_tag="KJL99_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HBD4436270.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            3646..4971
                     /gene="rimO"
                     /locus_tag="KJL99_001494"
     CDS             3646..4971
                     /gene="rimO"
                     /locus_tag="KJL99_001494"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HBD4436271.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ANMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLMSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(5018..6346)
                     /locus_tag="KJL99_001495"
     CDS             complement(5018..6346)
                     /locus_tag="KJL99_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBD4436272.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLVMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVILGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(6354..8696)
                     /locus_tag="KJL99_001496"
                     /pseudo
     CDS             complement(6354..8696)
                     /locus_tag="KJL99_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(8874..9785)
                     /gene="gsiD"
                     /locus_tag="KJL99_001497"
     CDS             complement(8874..9785)
                     /gene="gsiD"
                     /locus_tag="KJL99_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HBD4436273.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGVAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(9788..10708)
                     /gene="gsiC"
                     /locus_tag="KJL99_001498"
     CDS             complement(9788..10708)
                     /gene="gsiC"
                     /locus_tag="KJL99_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HBD4436274.1"
                     /translation="MLYYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMFLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(10726..12264)
                     /gene="gsiB"
                     /locus_tag="KJL99_001499"
     CDS             complement(10726..12264)
                     /gene="gsiB"
                     /locus_tag="KJL99_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HBD4436275.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPTALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(12284..14155)
                     /gene="gsiA"
                     /locus_tag="KJL99_001500"
     CDS             complement(12284..14155)
                     /gene="gsiA"
                     /locus_tag="KJL99_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HBD4436276.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYSHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGSRCAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(14142..15107)
                     /gene="iaaA"
                     /locus_tag="KJL99_001501"
     CDS             complement(14142..15107)
                     /gene="iaaA"
                     /locus_tag="KJL99_001501"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HBD4436277.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            15311..16546
                     /gene="moeA"
                     /locus_tag="KJL99_001502"
     CDS             15311..16546
                     /gene="moeA"
                     /locus_tag="KJL99_001502"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HBD4436278.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTTQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKMILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVREWVEVEPFNALFGGL"
     gene            16546..17295
                     /gene="moeB"
                     /locus_tag="KJL99_001503"
     CDS             16546..17295
                     /gene="moeB"
                     /locus_tag="KJL99_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HBD4436279.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(17474..18136)
                     /gene="fsa"
                     /locus_tag="KJL99_001504"
     CDS             complement(17474..18136)
                     /gene="fsa"
                     /locus_tag="KJL99_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBD4436280.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVTKFEQDWQGAFGRT
                     SI"
     gene            18267..19166
                     /locus_tag="KJL99_001505"
     CDS             18267..19166
                     /locus_tag="KJL99_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HBD4436281.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            19172..21604
                     /locus_tag="KJL99_001506"
     CDS             19172..21604
                     /locus_tag="KJL99_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HBD4436282.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIMLVAVSE
                     HIERFAALARAMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVHVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            21750..22565
                     /gene="ybiV"
                     /locus_tag="KJL99_001507"
     CDS             21750..22565
                     /gene="ybiV"
                     /locus_tag="KJL99_001507"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HBD4436283.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            22717..23982
                     /locus_tag="KJL99_001508"
     CDS             22717..23982
                     /locus_tag="KJL99_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HBD4436284.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLNRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRLLPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(24223..25815)
                     /locus_tag="KJL99_001509"
     CDS             complement(24223..25815)
                     /locus_tag="KJL99_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HBD4436285.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFAEPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            26034..26954
                     /gene="ldtB"
                     /locus_tag="KJL99_001510"
     CDS             26034..26954
                     /gene="ldtB"
                     /locus_tag="KJL99_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HBD4436286.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVEHKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(27012..28129)
                     /locus_tag="KJL99_001511"
                     /pseudo
     CDS             complement(27012..28129)
                     /locus_tag="KJL99_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706695.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
     gene            complement(28126..28593)
                     /gene="mntR"
                     /locus_tag="KJL99_001512"
     CDS             complement(28126..28593)
                     /gene="mntR"
                     /locus_tag="KJL99_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HBD4436287.1"
                     /translation="MSRRAGTPIAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            28779..28907
                     /gene="mntS"
                     /locus_tag="KJL99_001513"
     CDS             28779..28907
                     /gene="mntS"
                     /locus_tag="KJL99_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HBD4436288.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            29179..30762
                     /gene="opgE"
                     /locus_tag="KJL99_001514"
     CDS             29179..30762
                     /gene="opgE"
                     /locus_tag="KJL99_001514"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HBD4436289.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSGDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(30810..31325)
                     /gene="ompX"
                     /locus_tag="KJL99_001515"
     CDS             complement(30810..31325)
                     /gene="ompX"
                     /locus_tag="KJL99_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HBD4436290.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(31378..31443)
                     /locus_tag="KJL99_001516"
     CDS             complement(31378..31443)
                     /locus_tag="KJL99_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4436291.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            31678..32565
                     /gene="rhtA"
                     /locus_tag="KJL99_001517"
     CDS             31678..32565
                     /gene="rhtA"
                     /locus_tag="KJL99_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HBD4436292.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVSAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVVILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            32864..33367
                     /gene="dps"
                     /locus_tag="KJL99_001518"
                     /gene_synonym="pexB"
     CDS             32864..33367
                     /gene="dps"
                     /locus_tag="KJL99_001518"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HBD4436293.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            33771..34517
                     /gene="glnH"
                     /locus_tag="KJL99_001519"
     CDS             33771..34517
                     /gene="glnH"
                     /locus_tag="KJL99_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HBD4436294.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFSTEPK"
     gene            34657..35316
                     /gene="glnP"
                     /locus_tag="KJL99_001520"
     CDS             34657..35316
                     /gene="glnP"
                     /locus_tag="KJL99_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HBD4436295.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            35313..36035
                     /gene="glnQ"
                     /locus_tag="KJL99_001521"
     CDS             35313..36035
                     /gene="glnQ"
                     /locus_tag="KJL99_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HBD4436296.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALGNVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            36296..38520
                     /gene="ybiO"
                     /locus_tag="KJL99_001522"
                     /pseudo
     CDS             36296..38520
                     /gene="ybiO"
                     /locus_tag="KJL99_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
     gene            complement(38517..39443)
                     /gene="rlmF"
                     /locus_tag="KJL99_001523"
     CDS             complement(38517..39443)
                     /gene="rlmF"
                     /locus_tag="KJL99_001523"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HBD4436297.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            39719..39979
                     /locus_tag="KJL99_001524"
     CDS             39719..39979
                     /locus_tag="KJL99_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBD4436298.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMLGTATIYK"
     gene            40244..>40544
                     /locus_tag="KJL99_001525"
     CDS             40244..>40544
                     /locus_tag="KJL99_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HBD4436299.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPG"
BASE COUNT        10095 a        10721 c         9866 g         9862 t
ORIGIN      
        1 gagcccgctt cgcctgcccc gagattcact tcgatcttat caccaaacca gacgcgctga
       61 gtcacaaagg tggcatcagg tttaattggc gtcacagttt caaagaagcg gaaaccccag
      121 gtcaataatt tctcagactc attaaaacgg atacggtcag ttttcgcccc cagcactacg
      181 gagattaaac gcatatcacc ctgggtagcc gaagcaacca gattatatcc cgcacctgcc
      241 gtggttcctg tcttcatgcc atcaacattc agattgctgc tccacagcag acggttacgg
      301 ttaggctgac gaattttgtt gaaggtgaac tctttctctt tatgaatggc gtactcttcc
      361 ggcacatcgt ggatcaatgc tttacccagc aatgccatat cgcgcgcggt actgaactgc
      421 cccggcgcat ccaggccgtg caccgtctgg aaggtagtgt tggtcagccc cagttttttg
      481 gcataaccat tcatcagacc aataaaggac tcctggctcc cggcaacgta atctgccagc
      541 gcaatacagg cgtcattacc ggactggata atcacacctt tgttcaagtc tgccaccgaa
      601 acctgatcgc ccggtttgag gaacattacc gatgaaccac gcagtgccgg atttcccgtc
      661 gcccaggcat ctttaccgac cgtcaccata tcggtgagtt taatcttatc ggccttaagc
      721 gcctgcccaa ccacatagct ggtcatgatt ttagtcaggc tcgcaggatc cagtttctca
      781 tccgcattgc cttctgccag cactttaccg ctggcgtaat ccattaaaat ccatgcacgc
      841 gcatccacgc tcggcgcttc aacggtttgt tcggccgcga atgccgttgg ggcaaaaagg
      901 aataaaaatg cagaacccgc tgcaagacca cgaaggagag aggagtattg cgtcataaat
      961 gccacccgag tatccattca aaaaaatacg ttatatcgcc gtatgataag ttcggtgatt
     1021 aataacgcac acacaaactt aaagaaaccg taagttggta aagtttttaa agtttatgca
     1081 atatcctgcc gcttcgctgc ttctggtgtc atttttttgc cagctgttgc ttaaaagtta
     1141 actttacttc accatcggag tcctgttcag tctgtggctg cctgtcggaa aaggggtgag
     1201 tatgattacg ctgtggggtc ggaataattc aactaacgta aaaaaagtat tgctgacgct
     1261 cgaagaactg gaactacctt atgagcaaat tctcgcgggc cgtgagtttg ggataaatca
     1321 cgatgctgat tttctggcga tgaaccctaa cgggctggtg ccgttgttgc gtgacgacga
     1381 aagtgatctc attctttggg aatcaaacgc cattgtccgc tatctggcgg cacagtacgg
     1441 gcaaaaacgc ctgtggatcg actcaccggc acgtcgtgcg gaagcggaaa aatggatgga
     1501 ctgggcaaac cagacgctca gtaatgctca tcgcgggatc ctgatgggat tagtcagaac
     1561 accaccggaa gagcgcgatc aggccgccat tgatgccagt tgcaaagagt gcgacgccct
     1621 gtttgccctg ctcgatgcgg aactggcaaa agtaaaatgg ttctctggcg acgagtttgg
     1681 tgtgggcgat atcgctatcg caccgttcat ctacaatttg tttaacgttg gcctgacctg
     1741 gacaccgcgt ccaaatctgc aacgctggta tcagcaactc actgaacgcc ccgcagtgcg
     1801 caaagtggtg atgattcccg ttagctaatt gcgtgggcta actttaagta attccccact
     1861 ggactcgtcg gtgagaacgt ataaataacc gtcgggtcca gtgcgaacat cacgaattcg
     1921 ctgccctctg tccgttaaaa tacggccatc ttctgtcact ttgtcgccgt tgacgctcat
     1981 cacaatgaca tctttatctt tcagctccca ataaataatt tttgctgcca ctgggggaat
     2041 ttatcgctgt tatagaaggc catgccgctc acagcgggcg aatctttcca gtaaaaaaca
     2101 ggttgctcgg tcccggcgac gatctcccct ttcgcttccg gtatcttaaa gcctgaatag
     2161 ttgattcccc aggttgccag cggccagccg tagtttttgc ctttttgcgg gatattaatt
     2221 tcatcaccac cgcgcgggcc atgttcattc agccacagtg cattactcca cggattcatc
     2281 gccattcctt gcggattacg aatgccataa gaccagatct cggcgcgcgc accggattcc
     2341 tttataaaag gattatcatc cgggatttcg ccctggtcgg tcagacgcac cagtttgccc
     2401 tgtaatttat ccagatcctg cgccgtcggg cgctgattgt tttcgcccag agcaataaaa
     2461 agataacctt taccgtcgaa taccagccgc ccgccaaaat ggttgccggt agacagtttt
     2521 ggcatctggc ggaagacggt gcggaagtcg gtcacttttg agagatcatc acttaagcgg
     2581 ccataaccca cagcagttcc ggctttgcca tcatcgccaa cttcggaata acttaaccag
     2641 atgcggcgag actgagcaaa atcaggcgct aaaaccacgt ccagcaggcc gccctgcccg
     2701 tgcgcccaaa cgtccggaac tccggaaagc ggcgcagata atccttttcc tgcttgccag
     2761 tgacgcaact cgccgccgcg cagagtgatt aacataccgt gattatcggg taaaaaggcc
     2821 agtgcccagg gatggtcgag tttgtcttgc agtacttcga catttaccgt tgcaggagcc
     2881 gcccagagag cggaagaaag acaaataagg ggcacaagga aaaaggattg tcgatgcata
     2941 acgcctccgg attgactctg gcttaaagcg tagtcagtgg aggagataac aaattcattt
     3001 ttacaaaaac ttaaacatga agggggagac gctttctccc ccttagtttt caggccttct
     3061 caagcatggc gtgcttctgc aggctctgga tactcagcgt taagctcatc agacaatttt
     3121 caagcttatc ggcgttgacg gtaataacag tcgggcaatc atggtgccca ctcatcaaac
     3181 atactgcggc tgtcgctaat gcttcttcag catgatgaag agcactccac tcttcctgat
     3241 ccagatgaag attcaaccgc agcgatttat cgtgcagttc gcgattcagt ttaaaaaagt
     3301 tatctcgtag atgattgctt tcgctgacgg acatgtatcc ttttgccttc ctcagttgca
     3361 cgtatgcagc gaggtcaacg cgtgcgtcga tcaaccggtt cagcagatcg attcgctgtc
     3421 tgtcaacgaa catacttcga tcctcctctt tccagccttg ttgcaattaa atagtatggc
     3481 tattttttga gcaaaatgct gtccaggatc aattcttcac gccgaattaa caaaatttac
     3541 atttccttcg cctccgccag cgaatgagat tgcgatacaa attttatcca gaactctgta
     3601 gaatccctgc cctgaccttt cctgtaactg aatatttttt aagctatgag caaagtaact
     3661 ccccagccga aaatcggctt tgtttccctt ggctgtccga aaaaccttgt cgattcagag
     3721 cgtattctca ccgaactccg cactgaaggt tatgacgtgg taccgagcta tgacgatgcg
     3781 aacatggtga tcgtcaacac ctgcggcttt attgacagcg cggtacaaga atcactggaa
     3841 gccattggtg aagcgttgaa tgaaaacggc aaggtaattg tgaccggttg tctgggggca
     3901 aaagaagatc agatccgcga agtccacccg aaagtgctgg aaatcaccgg gcctcatagc
     3961 tatgagcagg ttctggagca cgttcatcac tacgtgccaa aaccgaaaca caacccattc
     4021 ctgagcctgg tgccagaaca aggtgtgaaa ctgacgccgc gtcattatgc ctatctgaaa
     4081 atttcggaag gctgtaatca ccgctgcacc ttctgcatta ttccgtctat gcgcggcgac
     4141 ctgatgagcc gtccgattgg cgaagtgtta agtgaagcga aacgtctggt agatgcgggc
     4201 gttaaagaga ttctggtgat ctcgcaggat acttccgcct atggcgttga tgttaaacat
     4261 cgtactggct tccacaacgg cgagccggta aaaaccagca tggtcagcct gtgcgaacag
     4321 ttatcgaaac tggggatctg gacacgtctg cactacgttt acccttatcc gcatgtggac
     4381 gacgtcatcc cactgatggc agaaggcaaa atcctgccgt atctggacat tccgttgcag
     4441 cacgccagcc cgcgcattct caaactgatg aagcgtccgg gttctgtaga tcgccaactg
     4501 gcgcgcatca aacagtggcg cgaaatctgc ccggaactga ccctacgctc aacctttatt
     4561 gtcggcttcc ctggcgagac ggaagaagat ttccagatgc tactcgactt cctgaaagaa
     4621 gcacgtctgg atcgcgttgg ctgctttaaa tacagcccgg ttgaaggtgc agacgccaat
     4681 gccctgcctg accaggttcc ggaagaagtg aaagaagaac gctggaaccg tttcatgcag
     4741 ctgcagcagc agatttccgc cgagcgcctg caagagaaag tgggccgtga aattctggtg
     4801 attatcgacg aagtggacga agaaggcgcg attggtcgca gcatggcaga tgcaccggaa
     4861 atcgacggcg cggtttatct caatggtgaa accaacgtta agccgggtga tatcctgcgt
     4921 gtgaaagtcg agcacgccga tgagtacgat ttgtggggta gccgggttta attaattcag
     4981 gccgggcaaa atcatttgtc cggcctgcga ataatgatta ctgccagcta atctgtgccc
     5041 tgccgttttt cttactctta tacagccgct catccgccag cattttcaag cgttcgacgg
     5101 tagtacattc atttgaatgc gctacaccga tactggtgct taaattaatg cttcccacct
     5161 ggaaatgaac gatttctttt agtatcgata gcaactcttc cagcgtgccg ccagtataaa
     5221 tcaccgcaaa ttcgtcacct ccgaagcggt agactttacc tttatctacc gggatctgtg
     5281 atttaaggat tttcaccact ttcatcaaca cttcatcgcc cagcaaatga ccgaaggtgt
     5341 cgttaatgtt tttgaattta tcgacgtcaa acagacacaa ataaccgcct ttttccggca
     5401 gcgcttttaa atcttcatca aaggattttc tgcccattgc tttggtcagg tcgtccagga
     5461 acgccgagtt atacatactg tagagttttt tgacatcggc agccgaaaag aaaagcccgc
     5521 catccagtac gcgttcaaaa tggttattaa tgatggcttc tttctccaca ataccacgat
     5581 taatcactct gcgtaccaga tagaggcaac tggcacccaa tatcactgcc accaatgcgg
     5641 caaagaaaat aatcaatgta caaacccgca aataaacatc ttttttatac gcaaatacgt
     5701 acaatccggt cggcaaatag gcgaagtgct ccatcgcttc cgaaacggtg aagatggcgt
     5761 ttttagcatt atattcatca ccggtcaccg gtttaccgtt cttaaataga gagaacatca
     5821 ggttctcgct gtccatcacc agcgattttg acagtaaaga aaaacgctcc agaccaagca
     5881 aaaatatcag actacgattg tgagttaccg gatagaggta atagacgaag gttttaccgt
     5941 caaaagagat aaaccctgta tttttgacat gtcgatactt cacaacctgc ataatatctg
     6001 cacaataatt tttctcgaac tcctttacct gaatacattc gtcactggaa atcagataag
     6061 gaccgacatc gtacaaataa gagggggaaa ccgagtcggc gtaagctgtg cgcaaaccaa
     6121 ccaacagctg ttcctccgtg ggatttagcg tgttgataag atcaattgcc acgctacgcc
     6181 gatattcagc aatatttgcc accgcaaaag cagtatcaat aacggactgt ttcaggctat
     6241 acacccgttc ctttaccatt tgagtgtaga tcccgcaggc aactgctgaa atcatgataa
     6301 aaatcagcag actgactgta agtacgaact tattgattct ggacatacgt aaactactct
     6361 tttactaatt ttccactttt atcccaagcg gagaatggca tgggtttctg gtacagatac
     6421 ccctgagcct gaacacatcc taatttaatt aattcgcgcg caacgtcttc gctctctacg
     6481 ccttccgcaa tcaccgggat ccggttatag cgtgccagat cgaaaataat gcgcacaatt
     6541 tttcccgcag taccgaaaat atcgccggta aattgcgagt ccacctttat gctgtctgga
     6601 ttaatttcat aaaaacgttt caggtttgac aaaccggtgc caaagtcatc aatcgatatc
     6661 cgtacccctt gtttacgcag ttgttcaata ttgagataaa aatgttcatt aattcgctcg
     6721 atcacggttt cagtgatctc aacacagagg ctgttcgcct tcaggccatt ttctttcatg
     6781 atggtggtaa aacgctcatg aaagtgcggt tcgtttaact gccagggcga aacattaata
     6841 tgcagtaaga aatcatcctt cagcgacgca cgttgactaa aatgtctgaa ttaacccatt
     6901 gccgttttaa taatctgata acccagctca ttgataaacc cgatatcttc agcaatggta
     6961 atgaacttca gcggtgaaat gatcccccgc tccgcagaaa cccaacggca caacgcctcc
     7021 gcgctcagaa tggcgcgggt gttaatatca acgatcggtt gatagacgag gaatagttcg
     7081 ccaatttcca ttgcatggcg aatattacgc gcaatccaga tattctcttc tatttcgttg
     7141 agtttcgcgt catcacagaa aataaactcg gagaacatat ggtcacggat actggaaatc
     7201 gccagtttca gtttttctgc gtaactccag gcgttttccc cctgcaaagg ttcacaaatg
     7261 gcagacttat ggatgcgcag attgcggtag ccaaagctgc tgtcaaaatc gtttaccatc
     7321 gctattttac gggtaaatgt ctgtacattt tcccgacaca gaagtaaata gagatcggca
     7381 ttgaatgtca cgatatcgca gcattgcgca tatttctcgg caattcgctg acacaatttc
     7441 ctgacgattt tattgcgttc atcaacacca aacaccgcct ccacggcttc cagaccacac
     7501 agtttgatga tccccacagc cagatactga ttattgttgg actcaataat cgccatattt
     7561 ttaataaaac cgctttcctg atcgtaagag atcagtgaga gcaatttatt tgaccgttca
     7621 atttcggcat ctttcatttc cgcgatcgaa tttttgatgc tgataatctc tttcgcatcg
     7681 ccttcgcgaa ttttcagagc ctcgatctga tccgttgagt cgggcaaaaa cgccagcgta
     7741 ttaaccagct cggtcatttc agaaaattgc cgacggaaca cacgagtggt catgaaatat
     7801 accaccagca acacgataat aatgaacgca aacggtagat aaaaaagttt atctgatgcg
     7861 ttcaataaat taccgccagg ataataaaaa tagatttcga acaatgcatt atttggcact
     7921 gctgatgtct ttgcaatata ctccgtatcg acagaatcaa taatattata attaccttcg
     7981 ctattataaa taatcagttt ttcacgagcg agttgattct tggcaatgat ctcatgacct
     8041 tgtttcaaaa atagaacttc atcattagcg ctgatttgat caagatactc gttcagatac
     8101 cgccagttta aatcgacatg aattttatct accgctaaca aatcgtgatc tttgccgttg
     8161 atacgtagcg taaaagagat acatttttta cccggacgat caaagcaatt gtatgccgga
     8221 ttccagctgt tgttagagtc atctgaagcc accgaccacg gtcgtttcga tgggttaaaa
     8281 tcggcaaaaa taacccgccc tgactcgtcg acaagcggtc catttacttg ttcctgatga
     8341 taaacaacga ttttatcatt cgccctccgc gaaaagagat aatatttatt atcatcaagt
     8401 accagtccca ccgagtctaa aaatggcatc atttccattt tcagaatcac ggcgcgttga
     8461 atctcctctg tattttttaa taaatacagt gagtccttta atgtcagggc cgaacgttca
     8521 atatctttaa agaaatagcc aacgtttttc tgaataagtt caaagcggct atcggcacgt
     8581 tttaccgcgc gttccgatac cagttgatag ttaatcatga aaaaaagacc aataaatgac
     8641 agtgctgcca ataaaaataa aattatcagc cttatctttt cgtataaact cagcatgtta
     8701 atacatttaa caaaataact atctgaacgc tgtgccgctg caaaaatagt cccttgctgc
     8761 ggctttttaa ccaaattacc cgattctaca aaatcagcag aaagttacaa cacttgctta
     8821 gggcaataat ttcctgtgag gtgattaccc tttcaagcaa tattcaaacg taactatcct
     8881 ttaattttcg gatccagcgc atcgcgtaaa ccatcgccca acaaattgaa cgccagtacg
     8941 gtcagaaaaa tagccagggc cggaaaaaca gcgacatgcg gcgcgataac catatccgct
     9001 cgtgcctcat tgagcattgc tccccactct ggtgtcggcg gctgcgcacc gaggccgaga
     9061 aatgacaggc tggcggcaga gataatcgag gtgccaatgc gcatggtgaa aaacaccacg
     9121 atagaagaga cggtcccagg caggatatga cgcaataaaa tggtcatatc gctggcacca
     9181 atactgcgtg ctgactcaat aaaggtttgc tgtttcaaca ccagcgtgtt gccgcgcacc
     9241 aggcgggcaa acgcggggat ggaaaaaatg gcgactgcga taatcacgtt agcaatgccg
     9301 cttcccaaca ccgcaacaac agcgatcgcc agtaaaatac ccgggaaggc aaacagcaca
     9361 tcgcaaatgc gcatgatcag ccgatcccac cagccttcat aatatccagc caacaagccc
     9421 agcaacgtcc ctatcgccac accgataaac acggcaaaca cgcccgccgc cagcgagatt
     9481 tgcgcaccaa ccaggacacg gctgaaaatg tcgcgcccca gtgaatcgac gccaaaccag
     9541 tgctgcaaag aaggtccgtt attcagattg tcataatcaa aataattttc ggcgtcatag
     9601 ggagcgatcc agcgtgcaaa aatggccacc acaatcaata aaataacgaa taatgcggcg
     9661 gtcatcgcca tatgctgacg gcgaaatcgt cgccagaatt catgccacgg tgtacgtacc
     9721 tggtcaggtt tgaccagtgg catggcgttt aacaccgcct gacgtcgcca gttaaatagt
     9781 cgcatcctta cttgtacctg atagccgggt taatggcggc gtaaagcaca tccaccacta
     9841 agttgataag aataaattcc agcgagaaaa gcagaatttc cgcctgaatc accgggtaat
     9901 cgcgcatttc tacagagtca acgagtaagc gtccaagtcc gggccagttg aaaactttct
     9961 ccaccacgat agaaccaccg agcaaaaagc caaactgtaa gcccatcatg gtcactaccg
    10021 ggatcatcgc gttgcgtagc ccgtgtttga ggacaaccca ggtttcgctc acccctttcg
    10081 ccctcgcggt acgcatataa tcttcgctta aaacgtcgac aaatgacgca cgggtaaagc
    10141 gcgccatcac ggcggccact gccgcgccga gcgtcaggga gggtaaaatg tagtgctgcc
    10201 aactgtctgc tcccacagta ggcagccagc ccagttcaac ggagaatacc tgaattaaaa
    10261 acatccccag cgcaaatgcc ggaaacgaga tccccgacac cgcaatggtc atactcaatc
    10321 gatccggcca acgattacgc cagacggcgg cgataattcc cgccgccata ccaaatatca
    10381 ccgcccagac catactggtt atggtcagcc atagcgttgg cataaagcgg ctggcaatct
    10441 catcggcaac cggacgacgc gacaccatcg acaggccaaa atccccctgc acagcattgc
    10501 tgatatagtg ccagaactgg tgatacagcg gctgatccaa ccccagctgc tgacgcacca
    10561 gttctataac ctgcgcatca gcttcgggcc cggcaatcaa tcgcgccgga tcgccgggca
    10621 gcatgtggac aaataaaaac accagcaccg agacgataaa cagcgtcgga atcaacccca
    10681 gtaatcgttt gataacgtaa taaagcattc cactccctgc gttgcttatt gcaaatccgc
    10741 gtcttcaaag ctgaagccgg tatctggcat gatccaaaaa ccggtcaggt ttttactgtg
    10801 tgccgacacc agtttttcta ccaccagcgg gatccacggc gattcttgcc agatgatatc
    10861 ctgcgccgcc ttatataagc gggtcttttc cgccggatca ttcgttttca gtgcctgagc
    10921 caggaagtca tccacctgtt tattgctgta aaacgcggta ttaaacagcg tcggtggcca
    10981 gttttgcgag gcaaacagcg gcgatagtgc ccagtcggct tcgccggttg aagccgacca
    11041 gccagtgtag aacatccgca cgccactctc tttttgccct ttaccttcaa cttctgccgc
    11101 ccgctgtccg gcatccatcg cggtcacctg ggctttaatc ccgacctgcg ctaactgctg
    11161 ctgggtaaat tgcagcactt tctgcgcggt gctgtggtta tgtgacgacc acagcgtggt
    11221 actgaaaccg ttgggatatc ccgcctcttt cagtaattcg cgcgctttca ctggatcgta
    11281 aggccacggt ttataacttt gcgcgtaggc gatacttggc ggtaccacac cagtagctgg
    11341 agttgcatag cccgcaaagg caactttcac cagcgccgga cggttaatgg cgtaattcag
    11401 cgcctcacgg accttcgggt tatcgaacgg cttttgcgtc acgttcatac tgatataacg
    11461 ctgcataatt gacggactgg ccatcaactc gatatttttg tttttctcca gcagtgtggc
    11521 ctgctcgtag ggaatgggga aagcaaactg cgcttcaccg gtttgcagca ttgccgcgcg
    11581 ggtgttgtta tccgccaccg gacgccaggt tatgctgtcc agtttgggca atcctggctg
    11641 ccagtaaccc gcgaattttt tcaccttcac aaaatcggtc tgattccagg tatccagttc
    11701 atacggtccg gttcccaccg gatgaaaacc aatctccttg ccatattttt ccagcgctgt
    11761 cggtgaaatc atcgcggtcg ccggatgggc aagaatatta ataaacgctg agaacggctg
    11821 tttgagggta atctttaccg ttgtcggatc gatcgcctcc gttttggcaa tattcttata
    11881 caggttatag cgtttaagat gattcgccgg atcgctggcc cggtccagat tcgcttttac
    11941 cgccactgcg ttgaaatcgg tgccatcctg gaatttaatt ccttcccgca atttcacggt
    12001 gtaagtaatg ccgtcatcgg aaacggtata actctccgcc agcacgtttt tcagtttcat
    12061 ctctttatcc agaccgaaca gcccctggta aaacgatttc gctacggcct gagataacgt
    12121 gtcattcgcg tcatacggat cgagcgtggt gaaattcgat cctaccgcca ccaccacatc
    12181 tttggcagcg aatgcacaag atgccatcaa cgctgtcgca atgcccagcg ccactaaccc
    12241 actacggtgt acagctcttg ccattttatt ctccgcctga atgttatcta cgcatgaatg
    12301 cgtattctga ttgtggttgt gcgacgtaat gccccggccc gacgcattgc aacgagacgg
    12361 ctgccacctc ttcgccacgc agatgaatat tgctgggaag atcgtccgac agcagtacac
    12421 gctgcggtcg ttgtcgggac ggttcagcga ccggaactgc cgccagtaat ttacgcgtat
    12481 aaggatgctg cgggttttcg aagaccgcgc accgtgaacc aatttcaaca atttgcccga
    12541 gatacatcac cgccacacga tgactaatcc gctctaccac agccatatcg tgggagataa
    12601 acagatacgc aatgccgaaa tcacgctgga gatcgagcaa caagttgata atctgcccgc
    12661 gaatagaaac atccagcgcc gaaacggctt cgtcggcaat gatcactttt ggattcaatg
    12721 ccaacgcgcg agcaatgcag atgcgctggc gctgaccgcc ggaaaactca tgcgggtaac
    12781 gccaggcatg ttcaggtaac aggcccacgc gctccagcaa ccacgcaacg cgtgcagccg
    12841 cgtctttacc tggcaataaa ccgtgtacac gcagcggttc gataatcgaa tcaccgatgg
    12901 tctgacgtgg gtccagcgaa gcgtaagggt cctgaaaaat aaactgaata tcccggcgca
    12961 atgcctgaag tttgccaggt gacaaggtat cgattcgctg accgttaaag ataatttcgc
    13021 cgccctgcga ttcgaccagg cgcagcaacg cccgcccggt agtggattta ccgctgccag
    13081 actcgcccac cagcgatagc gtttcgccag gccagagatc aaaactgact ttctcaacgg
    13141 catgcacttc ccgcgttacg cgattcaaca aaccgctgcg caaagggaaa cgggtgacaa
    13201 gattacgcac ccgtaaaaca ggttcgccat ccaccaccgt tttctgctcg atggggggtt
    13261 cctgtttcgc tggatgttca agcgatatca atgggaaacg tcggggataa tctaaccctt
    13321 tcatcgcacc aagttgcgga acagcagcta acagcgcacg ggtgtaagga tgttgcggtg
    13381 catgaaaaat ctgttcgacg gtacccgttt ccaccgcctc gccctgatac atcaccagta
    13441 cccgatcggc aatctctgcc accacgccca tatcgtgagt gataaagata acgcccatcg
    13501 acatctcttt ttgcaatact ttgattaatt gcaggatctg cgcctgaata gtgacatcca
    13561 gcgcggtggt tggctcatcg gcaatcagca ccgccgggcg gcatgacagc gccatcgcaa
    13621 tcatcactcg ctggcgcatc ccgccagaga gttgatgcga ataacgtgaa agaatggttt
    13681 gtgcctccgg aatgcgtacc tgatccagca tccgcttcgc ctcgaccatc gcttcttcac
    13741 gactggcgtt ctgatgcaga cgaattgatt cggcaatctg ttcacccaca gtaaatactg
    13801 ggttcagcga tgtcatcggc tcctgaaata tcatcgccat atccgcaccg cgcacatggc
    13861 gcatttgtgc agcgctctgc tcgctaagtt caatcacatc acgactgcgc cgccgcaaca
    13921 gcattttatc gcactgtact aaaccgcccg cctgttccaa cagacgcatc aatgccagcg
    13981 cagtcactga cttaccggag ccggattcgc ctacaattgc cagcgtctca ccgcgttgca
    14041 gactaaaaga gagattgcgg accgcagcta ttttctgctg gtcctgcata aaggcaatat
    14101 taagattttc aaccgccagc acattaccgg catcaagttc atcactgtgt ggcaacggtg
    14161 tccccttttt cacggtagat accggtggtt ggcgtatcgc ctgcgtagcc ccaggcgcga
    14221 tacattcctt cggtgttaaa cggtagcgcg acattccctt catggtcgat agcgattaag
    14281 ccaccgctac cgccaagcgc agggagtttt tccattacta cccgctcgca ggcttccgcg
    14341 agacttaatc cgccgtaatc cattaacgcg gcgatgtcat atgccgccag cgcgcggatg
    14401 aagacttcgc ctgtgccggt acaagaaacc gccacactgg cgttattggc gtagcatccg
    14461 gcacccacta agggactatc gccaactcgt ccgggtaatt tattggtcat tccgcctgtg
    14521 gacgtggctg ccgccagatt gccgtctaaa tccaacgcca cggcccccac ggtgcccatt
    14581 ttctgttttt catccagtgg cgcaccgcta tggtcgagga ctgttgcccc ttcctcgcgc
    14641 gctgccagta gttgttcata acgcaaaggc gtggagaaaa tctccggtga gacgcgctcc
    14701 atgccatgag caaacgcaaa attttctgcc ccttcgccaa tcatcatcac atgcgggctt
    14761 tgctccatca ccagccgggc ggcaagaacc ggattacgca gatgactaac gcccgccacc
    14821 gcaccggctt tcagggtgtt accatccatc acacaggcgt ccagttcatg ggtttcatca
    14881 cgcgtaaaga cagcgccaat tccggcgtta aacagtggac actcttccag cagacgcacc
    14941 gcttccgtca ccacatccag cgcactttcg ccggcttcca gcattttctg cccggtttca
    15001 acaatggcag acaacgcctc gatgtagcgt aattcctgtt gcagactcat ctgcgcgcgg
    15061 ctaattgcac ctgcgccacc atgaattgca atgactgctt tgcccatagt attcgtccat
    15121 taagggtggc tcgccacttt tgctataaat atcagaattt ttatgaatta ctcattcaat
    15181 ttttgaatat agaaagatgg atatggcatg taaaggcagg agccaacgct acgcccgagg
    15241 tggcatacag tctatggcgt ttttctgaca taataggcaa attcgatttt gcctccgcag
    15301 gagtgttttc atggaattta ccaccggatt gatgtcgctc gacaccgcgc ttaatgagat
    15361 gctttctcgc gttaccccac tgaccaccca ggaaacgctg ccactggttc agtgttttgg
    15421 tcgtattctg gcgagcgatg tcgtttcgcc actggatgtc ccggggtttg ataactccgc
    15481 tatggacggc tacgcggtgc gtttagccga tattgcctcc gggcaaccgc tgcccgttgc
    15541 cggtaaatcc tttgccggtc agccatacca tggtgaatgg cctgcgggta cctgcattcg
    15601 tattatgacc ggtgcgccgg tgccggaagg ctgcgaagcg gtggtgatgc aggagcagac
    15661 tgaacaaacg gacaatggcg tgcgttttac tgctgaagtg cgtagcgggc aaaatattcg
    15721 ccgtcgcggt gaagatatct ctgcaggtgc ggttgttttc ccggcgggaa ctcgcctgac
    15781 taccgcagag ctgccagtaa ttgcttcact ggggattgcc gaagttccgg tgattcgtaa
    15841 agtgcgtgta gcgctttttt ctaccggtga tgaactccag ttgcccggtc agccgctggg
    15901 cgacggccaa atctacgaca ccaaccgtct cgccgtacac ctgatgttag aacagctggg
    15961 ttgcgaggta atcaacttag ggattatccg cgacgatcca catgccctgc gcgccgcatt
    16021 tattgaagcc gacagccagg cggatgtagt gatcagttcc ggcggtgttt cagtgggtga
    16081 agcggattac accaaaatga ttcttgaaga gctgggggag atcgccttct ggaagctggc
    16141 gattaaacca ggtaaaccgt tcgcgttcgg taaactcagc aatagctggt tctgcggcct
    16201 gccgggcaac ccggtttcag cgacgctgac cttctatcaa ctggtacagc ctttgctggc
    16261 aaaactaagc ggcaacaccg ccagcggcct gcccgcgcgc cagcgcgtac gcacagcgtc
    16321 ccgcctgaaa aaaacgccag gacgtcttga tttccagcgc ggcgtgctgc aacgcaacgc
    16381 cgacggtgaa cttgaagtga cgaccaccgg acatcagggt tcacatatat ttagctcctt
    16441 tagtctcggc aactgcttta tcgtgctgga acgcgatcgc ggcaatgtgg acgtgcgcga
    16501 atgggtggaa gtagaaccgt ttaacgcgtt gttcggaggc ctgtaatggc ggaactcagc
    16561 gatcaggaga tgctgcgcta caaccggcaa atcattctgc gcggttttga ttttgacggt
    16621 caggaggcgc taaaagattc tcgtgtgctg gtagtcggcc tcggtggact cggctgcgca
    16681 gcttcgcagt atctggcaag cgccggtgtc ggcaacctga cgctgctcga cttcgacacg
    16741 gtttcgctct cgaatctgca acgccagaca ctgcacagtg atgccacggt cgggcaaccg
    16801 aaggtggaat ccgcccgtga cgccctgacg cggatcaacc cacatatcgc gattacgcca
    16861 gtcaatgcac tgctagatga cgcagaactt gcagcattga ttgctgaaca cgatctggtg
    16921 ctcgactgta cggataacgt tgcggtacgt aatcaactga acgcaggctg ttttgccgcg
    16981 aaggtgccgc tggtttccgg cgcggcaatt cgtatggaag ggcaaatcac ggtcttcact
    17041 tatcaggacg gcgaaccgtg ctatcgctgc cttagccgtt tgtttggcga gaacgcgtta
    17101 acctgcgtag aagcaggcgt gatggcaccg ttgataggcg taattggttc gttgcaggca
    17161 atggaagcga tcaaactgct ggcaggttat ggcaaaccgg ccagcgggaa aatcgtcatg
    17221 tacgatgcga tgacctgtca gtttcgcgaa atgaaactga tgcgcaatcc ggggtgtgag
    17281 gtgtgcgggc agtaattgcc acctccaagc ctgataagac gcgacaagcg tcgcatcagg
    17341 catttgctcc agacgccaga tgcggcgtga acgccttatc tggcctacgg ttcgggcaca
    17401 gatttccaag cgcggcaggc atcggtgcac tccgccacgt catcagcaat ttatgaggat
    17461 gcagaacgtc cggttaaatc gacgttctgc caaacgctcc ctgccagtcc tgctcaaact
    17521 tcgtcacagc ggcttccacc gccggatagc taatcatctg ttgtgccaca tccagtggca
    17581 gagtaattga ttcacatccg gccagtaagc agtccagcgc ctgacgcggg gttttgaaac
    17641 tcgctgccag cactttcgcc tgcggcgcat gcattttcaa taactggtgt aagtcggtca
    17701 cagtctgaat gccgctaccg ccctgagcat caatacgatt aacgtaaggc gcaacatatt
    17761 ccgcacctgc cagcgccgac agcagccctt gtgccgcgcc atataccgcg gttcccagcg
    17821 tcggaatccc ttccgctttt aacatcttaa tcgctgccag cccctcggct gtcaccggaa
    17881 ctttcaccac gatatccgca ataatagaac gcagcttaag cgcgtcattc accatccctt
    17941 cagcagtggt agccattacc tgggcaaaca gacgcccctg accgcccatc gcttcatgaa
    18001 gttgcggaag cacaacatcc agcggttttt tacccgcggc gataatgctt gggttagtgg
    18061 tcacacccgc cagcggaaaa atacgtgaca gcgccttcac cgcaacaacg tctgaagtat
    18121 ccagatacag ttccataacc atcctcaaaa tgtacttttt gtctgaatgt agactatcac
    18181 gggaaaactc tctgaacagt tgacgcacat caatctaact ttcattcgaa agtattttaa
    18241 tgtttgaacg aaacagagag gcaactatga ttttcaatat tcagcgctac tcgacccatg
    18301 atggccccgg tatccgcacg gtcgtatttc ttaaaggctg ttcgctgggc tgccgctggt
    18361 gtcagaaccc ggaaagccgc gcccgtacgc aggatctgct gtatgacgca cgactgtgtc
    18421 tggaaggctg cgagctgtgc gctaaggccg cgccggaagt gattgagcgc gcgctgaatg
    18481 gtttgcttat tcatcgggaa aagttaaccc cggagcatct gacggcgtta accgactgct
    18541 gtccgacaca ggcgttaacc gtgtgtggtg aagtgaaaag cgttgaggag atcatggcga
    18601 ccgttctgcg cgataaaccg ttttacgatc gcagcggcgg cggcttaacg ctttcgggtg
    18661 gtgaaccctt catgcagccg gaaatggcga cggcgctgct gcaagcctgc cacgaggcag
    18721 gcattcatac tgcggtagaa acctgtctgc atgtgccgtg gaaatatatc gccccttctc
    18781 tgccctatat cgatctgttt cttgccgatt taaaacacgt tgccgacgcg ccgtttaaac
    18841 agtggaccga cggtaacgcc gccagggtgc tggataacct gaaaaaactc gccgcagcgg
    18901 gcaaaaaaat cattatccgc gtgccgctga ttcagggctt taatgccgac gaaacctctg
    18961 taaaagccat taccgatttt gccgccgacg agctgcacgt tggcgaaatt cattttctgc
    19021 cctaccacac gctgggcatc aacaaatatc acttacttaa tctgccctat gacgccccgg
    19081 aaaaaccgct tgatgcgcca gagctgctcg attttgccca gcagtatgcc tgccagaaag
    19141 ggttaaccgc gaccttacga ggataacaac catgaccaca ctgaaactgg acacgctcag
    19201 cgaccgcatt aaagcgcaca aaaatgcgct ggtgcatatt gtgaagccgc cagtctgtac
    19261 cgagcgcgcg cagcactata ccgagatgta tcaacaacat ctcgataagc cgatcccggt
    19321 acgtcgcgcg ctggcactgg cgcatcacct ggcgaatcgc accatctgga tcaaacacga
    19381 tgagttgatc attggcaacc aggcaagcga agttcgcgcc gcgccgatct tcccggaata
    19441 tactgtctcg tggatcgaaa aagagattga tgatctggca gatcgtcccg gtgctggctt
    19501 tgcggtgagc gaagagaaca aacgtgttct gcatgaagtg tgcccgtggt ggcgcggtca
    19561 gacggtacag gatcgctgct atggcatgtt taccgatgag caaaaaggtc tgctggcgac
    19621 cggcatcatt aaagcggaag gcaatatgac ctccggcgat gcgcacctgg cggtcaattt
    19681 cccgctgctg ctggaaaaag ggcttgatgg tctgcgtgag aaagtagcgg aacgtcgctc
    19741 gcgcatcaat ctgacggtgc tggaagattt gcacggcgaa cagttcctga aagcgattga
    19801 tatcatgctg gtggcagtca gtgaacacat tgaacgtttc gctgccctgg cgcgtgcaat
    19861 ggccgcgacc gaaacccgcg aaagccgtcg cgatgaactg ctggcgatgg cagaaaactg
    19921 cgatcttatc gcccaccagc cgccgcagac tttctggcag gcgctgcaac tgtgttactt
    19981 catccagttg attttgcaga ttgaatctaa cggtcactcg gtgtcgtttg gtcgtatgga
    20041 tcagtatctc tacccgtact atcgccgcga cgttgaactg aaccagacgc tggatcgcga
    20101 acacgccatc gaattgctgc atagctgctg gttgaaattg ctggaagtga acaaaatccg
    20161 ctccggctcc cactcaaaag cctctgcggg aagtccgctg tatcagaacg tcaccattgg
    20221 cggacaaaat ctggttgatg gtcaaccaat ggacgcggtg aatccactct cttacgcgat
    20281 cctcgaatcc tgcggtcgcc tgcgttccac tcagcctaac ctcagcgtgc gttaccatgc
    20341 aggaatgagt aacgatttcc tcgacgcctg cgtacatgtg atccgctgcg gcttcgggat
    20401 gccggcattc aacaacgacg aaatcgtgat cccggaattt attaaactcg gtattgaacc
    20461 gcaggacgct tatgactacg cagcgattgg ttgtatagaa accgccgtcg gtggcaaatg
    20521 gggctatcgc tgtaccggca tgagctttat caacttcgcc cgcgtgatgc tggcggcgct
    20581 ggaaggcggg cgtgatgcca ccagcggcaa agtgttcctg ccacaagaaa aagcgttgtc
    20641 ggcaggtaac ttcaacaact tcgatgaagt gatggacgcg tgggatacgc aaatccgtta
    20701 ctacacgcgt aaatcaatcg aaatcgaata tgtcgtcgac accatgctgg aagagaacgt
    20761 gcacgatatt ctctgctcgg cgctggtgga tgactgtatt gagcgagcga aaagtatcaa
    20821 gcaaggcggc gcgaaatatg actgggtttc tggcctgcag gtcggtattg ccaacctcgg
    20881 caacagcctg gcggcagtga agaaactggt gtttgaacag ggcgcgattg gtcagcaaca
    20941 gctggctgcc gcactggcgg atgacttcga tggcctgact catgagcaac tgcgtcagcg
    21001 gctgattaac ggtgcgccga agtacggcaa cgacgatgat accgtcgata cgctgctggc
    21061 tcgcgcttat cagacctata tcgacgaact gaaacagtac cataacccac gctacggtcg
    21121 tggtccggtt ggcggcaact attacgcggg tacgtcgtct atctccgcta acgtaccgtt
    21181 tggcgcgcaa actatggcaa cgccggacgg gcgtaaagcc cacaccccgc tggcagaagg
    21241 cgctagtccg gcctctggta ctgaccatct cggccctact gcggtcattg gctcagtggg
    21301 taaactgcct acggcagcga ttctcggcgg cgtgttgctc aaccagaaac tgaatccggc
    21361 aacgctggag aacgaatctg acaagcagaa actgatgatc ctgctgcgta ccttctttga
    21421 agtgcataaa ggctggcata ttcagtacaa catcgtttcc cgcgaaacgc tgctggaggc
    21481 gaaaaaacat ccggatcagt accgcgatct ggtagtgcgt gtcgcgggtt attccgcctt
    21541 cttcaccgcg ctctctccag acgctcagga cgatatcatc gcccgtactg aacatatgct
    21601 gtaatctctt cggcccggct gcggcgtcgc cgggctaaat cattcacatc atcaatttca
    21661 tccttacttt cattcgaaat ataatttgtg ctctgcgtca cattgttatt acactgttac
    21721 ggtttcattt tcgagccagg agcacaccta tgagcgtaaa agttatcgtc acagatatgg
    21781 acggtacttt tcttaacgac gccaaaacgt acaaccaacc acgttttatg gcgcaatatc
    21841 aggaactgaa aaagcgcggc attaagttcg ttgtcgccag cggtaatcag tattaccagc
    21901 ttatttcgtt ctttcctgag ctaaaggatg agatctcttt tgtcgcggaa aacggcgcac
    21961 tggtttacga acatggcaag caactgttcc acggcgaact gacccgacat gaatcgcgga
    22021 ttgttattgg cgagttgcta aaagataagc aactcaattt tgtcgcctgc ggtctgcaaa
    22081 gtgcatatgt cagcgaaaat gcccccgaag catttgtcgc actgatggca aaacactacc
    22141 atcgcctgaa acctgtaaaa gattatcagg agattgacga cgtactgttc aagttttcgc
    22201 tcaacctgcc ggatgaacaa atcccgttag tgatcgacaa actacacata gcgctggatg
    22261 gcattatgaa acccgtcacc agtggttttg gctttatcga cctgattatt cccggtctac
    22321 ataaagcaaa cggtatttcg cggttactga aacgctggga tctgtcaccg caaaatgtgg
    22381 tagcgattgg cgacagcggt aacgatgcgg agatgctgaa aatggcgcgt tattcctttg
    22441 cgatgggcaa tgctgcggaa aacattaaac aaatcgcccg ttacgctacc gatgataata
    22501 atcatgaagg cgcgctgaat gtgattcagg ctgtgctgga taacacatcc ccttttaaca
    22561 gctgacctct ctaccggagc catgcgctcc ggttttccct tctttttaca tcctgtacat
    22621 cacatcaaat cattaaactt gcattaactt atttataact tgtattgtca catgcaaatt
    22681 agcatagttt cgaatgaaag ttaatgcaag gtggttatgg cttctacttt taccagcgac
    22741 acattgcctg ccgatcacaa agcagctatc cgtcagatga agcacgcgct gcgggcgcag
    22801 cttggcgatg tccagcagat ctttaatcag ctaagcgatg acattgccac gcgagtggct
    22861 gaaatcaacg cactcaaagc acagggcgat gccgtctggc cggtgctgtc ttatgccgat
    22921 atcaaagcag gtcatgttac tgcagagcag cgcgaacaga ttaaacgtcg cggttgtgcg
    22981 gtgataaaag gccatttccc ccgcgaacaa gcgctaggct gggatcagtc gatgctggac
    23041 tatctgaacc gcaaccgctt tgacgaggtc tacaaaggcc ccggcgataa tttcttcggg
    23101 acgctcagcg cttcacgtcc cgagatttac cccatctact ggtcgcaggc gcaaatgcag
    23161 gcccgccaga gtgaagaaat ggcgaatgcg cagtcgtttc tcaatcgtct gtggacattt
    23221 gaaagtgatg gaaagcaatg gtttaacccg gatgtgagcg tcatctaccc tgaccgtatc
    23281 cgcctccttc cgcccggaac gacctccaaa ggtcttggag cgcataccga ctccggggcg
    23341 ctggaacgct ggctgcttcc agcgtatcag cacgttttcg ccaacgtctt taatggcaat
    23401 ctggcgaaat acgatccctg gcatgcggca catcgtacgg aagttgaaga gtacacggtg
    23461 gacaacacca ccaaatgttc cgtgtttcgg acattccagg gctggactgc gctctcggat
    23521 atgctgcctg gtcaggggtt gctgcacgtc gtgcccattc ctgaagccat ggcgtacgta
    23581 ctgttacgtc cgctgcttga tgatgtgccg gaggatgaac tgtgcggcgt agcgcccgga
    23641 agagtgttgc cggtatcaga gcaatggcat ccactgttaa ttgaggcgtt aaccagcatt
    23701 ccaaaactcg aggccggaga ctccgtctgg tggcactgcg acgtcatcca ttccgttgcc
    23761 cccgttgaaa atcaacaggg ttggggcaac gtgatgtaca ttcctgcggc accgatgtgc
    23821 gagaaaaatc ttgcctacgc gcacaaggtg aaggccgcac tggaaaaagg cgcatcgccg
    23881 ggcgacttcc cgcgcgagga ctatgaaaca aactgggaag gacgctttac gcttgccgac
    23941 ctcaacattc acggtaagcg agcgttgggc atggatgttt gagagttaat ggaagtgaat
    24001 tgatgtggga ttaagaaaat cgattcgacg ttactcagat tgctgacaag tgcgctttct
    24061 taatgcctga tgcggcgtta acgccttatc cggcctacaa agtcctgcag attcaatata
    24121 ttacaggtac tgcgtaggcc tgataagcgt agcgcatcag gctattttgc atttgtcatc
    24181 agccgtcgtc accccgacag tatcggggtg acggataatc acttactcga tccctttact
    24241 acgcaggtaa tcttcgtaat taccgctaaa gtcgatcacg cgttccgggg tgatttccag
    24301 aatgcgggtc gccagggagc ttacgaactc acggtcgtgt gaaacaaaga tcagcgtgcc
    24361 ctgatacagt tccagcgcca tgttcagtga ctcgatggat tccatatcca ggtggttggt
    24421 cggttcgtcc atgatcagaa tgttcggctt ctgcatcatt aacttaccaa acagcatccg
    24481 ccctttttcc ccaccggaaa gcactttagc tggctttttg atgtcgtcct ggctgaacag
    24541 caaacgaccg agaatgctgc gtaccgcctg ctcgtcatcg ccttcctgct tccactgact
    24601 catccattcg aacacggtca gatcattttc aaactcatat tcgtggtcct gggcgtagta
    24661 accaatgcgc gcgttttcag accattttac ggtgccgctg tccggttgca gatcgcccac
    24721 cagcgttttc agcagcgttg atttaccgac gccgttggta cccagtaccg caagtttttc
    24781 accgacctcc agcagcaggt tgagattttt aaacagtgga ccgttatcaa accctttggt
    24841 cagaccttcc acttccagcg cgttacggaa cagtttctta tcctgttcaa aacggataaa
    24901 cgggttctga cggctggagg ctttcacctc ttccagtttg attttatcaa tctggcgcgc
    24961 gcgcgaagtt gcctggcgag atttcgaggc gttggcgcta aagcggctaa cgaaagattg
    25021 caactcagca atctgcgctt tcttcttggc gttatcggcc agcagacgtt cacgcgcctg
    25081 ggtcgccgcc gtcatgtact catcgtagtt acccggataa acgcgcagct cgccgtaatc
    25141 cagatccgcc atgtgggtac agaccatgtt aaggaagtga cggtcgtgcg agatgatgat
    25201 catggtgctg tcacgctcgt tcagcacctg ttccagccag cgaatggtgt cgatgtcgag
    25261 gttgttggtc ggttcgtcga gcaggagaat atccggctca gcaaacagcg cctgcgccag
    25321 aagcacacgc agcttccagc caggagcaac ttcactcatc gggccgtagt gctgttccac
    25381 tggaattccc acgccaagca acagttcacc ggcgcgagct tccgcagagt aaccgtccat
    25441 ttcgccgtat ttcacttcca gatcggccac tttatagccg tcttcttcac tcatttccgg
    25501 caaagcatag atgcggtcgc gctcctgctt cacttcccac aactctttat gccccatgat
    25561 caccgtatcc agcacagtga actcttcaaa ggcaaactga tcctgacgca gcttaccaat
    25621 gcgctcgttg ggatcgaggg aaacgttacc cagcgtcggc tcaaggtcgc cgccgagaat
    25681 cttcataaag gtggatttac cactaccgtt cgcgccaatc aggccgtaac ggttgccgcc
    25741 gccgaatttg acggaaatgt tttcaaacaa cggcttactg ccgaactgca tggtgacgtt
    25801 actggaaact aacacaggcg tatcctgaaa agagatatga caaaccgcgc attatgccat
    25861 aagtctgaaa gaagatcctg ctcctcgctt aaggtctaca ctcaagccaa aggcaaaaaa
    25921 tgtgatttcg tacacatctg atttcactgt gagctggaat gaacttataa tgcgcttcca
    25981 atactctaat attctcaacc caatggcctg ccaggcacaa aatctcgctt aacatgaata
    26041 tgaaattgaa aacattattc gcagcggcct tcgctgttgt cggcttttgc agtaccgcct
    26101 ctgcggtaac ttatcctctg ccaaccgacg ggagtcgcct ggttggtcag aatcaggtga
    26161 tcaccattcc tgaaggtaac actcagccgc tggagtattt tgccgcggag taccagatgg
    26221 ggctttccaa tatgatggaa gcgaacccgg gtgtggatac cttcctgccg aaaggcggta
    26281 ctgtactgaa cattccgcag cagctgatcc tgccggatac cgttcatgaa ggcatcgtca
    26341 ttaacagtgc agagatgcgt ctgtattact atccgaaagg gaccaacacc gttatcgtgc
    26401 tgccgatcgg cattggtcag ttaggcaaag atacgcctat caactggacc accaaagttg
    26461 agcataagaa agcaggcccg acctggacgc cgaccgccaa aatgcacgca gagtaccgcg
    26521 ctgcgggcga accgctaccg gctgtcgttc cggcaggtcc ggataacccg atgggtctgt
    26581 atgcactcta catcggtcgc ctgtatgcta tccatggcac caacgccaac ttcggtatcg
    26641 gcctgcgtgt aagtcatggt tgtgtgcgtc tgcgtaacga agacatcaaa ttcctgttcg
    26701 agaaagtacc ggtcggtacc cgcgtacagt ttattgatga gccggtaaaa gcgaccaccg
    26761 agccagacgg cagccgttat attgaagtcc ataacccgct gtctaccacc gaagcccagt
    26821 ttgaaggtca ggaaatcgtg ccaattaccc tgacgaagag cgtgcagaca gtgaccggtc
    26881 agccagatgt tgaccaggtt gttcttgatg aagcgatcaa aaatcgttcc gggatgccgg
    26941 ttcgcctgaa ttaatcgtca aaacgtaaaa caaaggcgga ctcataatcc gcctttttta
    27001 tttgccagac cttaccaggc cgggatcata actaacaaaa cgtatcccac cagcgccgcc
    27061 cacaacagca tcggtattga atagagatgg aaacgccacc agatgcggcg atcgttcgcc
    27121 atacgtagcg caatcaaatt tgccagcgat cctggtaata aaccaaagcc ccccacgttt
    27181 accgcccatg ccagtaataa agacggaggt acataattga gcagcaaaat ggtactcggc
    27241 acgttactga tcacctgcga taaaccgact gccgttaacc ataacccggg ttcagataaa
    27301 tgactcacgt tacccaacac gccttgcaac gctggaagct gggtcagtaa atggacgtcg
    27361 ataaacatcg ccataaacac cagcagcaac gtccagtcca cactcaacac cacgcgacgc
    27421 gccagcagca caaagcccgc cgccacaatc accagtcccc acagctcttg tttgaactcc
    27481 agcgccgtca gaaagacgat atacagcccc agacaattcc acaccagccg cggtttccac
    27541 tccggtgttt gcacccccgt atggtattgc agcgcctttc cagggaaaca acaccagcac
    27601 aacagcagga gtgtcagcat cattgcgcca gccatcggtg ccatttgggc aataaatccg
    27661 gcaaacgaaa gaccagaacg tccccagata agaatatttt gcgggttgcc aattggcgtc
    27721 agtagcgaac cagcgttgac tgccagcgcc tcaaaaataa tcagccgatt aaccgggatc
    27781 tcacacagtc tttttagcgt gatagtcagc ggaacaacaa taaacagcgc gacatcgttg
    27841 gtcagaaagg tagaaagcag cgccgccgcc agcaccataa acatcgccag ccgacgctcc
    27901 gtagcaaagc ggcgcaccat tttgcgcccc agcacatcaa aataaccgct taactccaca
    27961 cctttggtca gcagcatcag gccgcttaag gtgatgatgg tgtgccagtc gatagcagca
    28021 ggccaggatt tcggtgcaaa gggcacgaaa aagcttaatc cgataccaac aagaattaat
    28081 aactgaaaaa acgatcgcct tgcagcgtgc gtaaaaaagg caggctcatt tggcaccgtg
    28141 tttctgggta aacaaacgaa aagcgtcgag cgtctcttca ctaacatggt gctccatgcc
    28201 ttccgcgtcg cgacgggcga tttccggact gacgcccaac accaacaaga aattttcgac
    28261 tatctgatgt cgctcgcggc tttcctgcgc cagcttctct ccttctgccg ttaaaaacac
    28321 gccacgccag gggatcattt caatcagccc catggttgcc agccgcttaa gcattttagc
    28381 caccgtcggt tgcgaaactc ccagacgagc agccatgtcc acctgacgag cttcccccac
    28441 ttccctgatc aagtcagaaa tcagctcaac gtaatcatca ataagctcgc gccgatgcgc
    28501 ctctctgacc tggcggaacc cttcaacgtg ctcttccacg ttcactaact gcgtcacttt
    28561 ttttgctatt ggcgtacctg cgcgacgact cattgtgctt cctcattatg gtgacgcata
    28621 catagcgttc tcatttcgtg cgcacattgt aaaccagagt tgcgaaggta caaaaaatta
    28681 acgttttagc aatagctata taatatagcc tgtgctatat ctgtatgtaa tgcaatcatc
    28741 cctcaaggat cgacgggatt agcaagtcag gaggtcttat gaatgagttc aagaggtgta
    28801 tgcgcgtgtt tagtcattct ccctttaaag tacggttaat gctgctctct atgttgtgcg
    28861 atatggtcaa caacaaaccg cagcaagata aaccttcaga taaatagcgg cgtcgcggta
    28921 cgccgcttca ctcctgcttt tatgcaggca taacgcgttt tggtctgaaa aaaaccactt
    28981 tttgtcggat ttgcaatccc cttcgcaaaa gatttgttcg tcagtagttg acctgaacgg
    29041 cggctcgctc tatcttcttg cagccctgcg tatattgcgg ctcgcggatg cggacccctt
    29101 tcccctcttc acgcactctt gcaggtattg acccttgacg ccagggtaag cacatggcgt
    29161 ttgttacgat agtggcatat gaatttaacc ctcaaagaat cgcttgttac ccgtagccgg
    29221 gtatttagcc cgtggactgc gttctacttt ttacagtcgc tattaattaa cctcggctta
    29281 ggttacccct tcagtttgct ctacaccgct gcgtttacgg ctattttgct tttgctatgg
    29341 cgaacattgc ctcgcgtaca aaaagttctg gtcggtgtca gttcgctggt ggcggcttgt
    29401 tatttccctt ttgctcaggc ctacggcgcg cctaatttca atacattgct ggcattgcac
    29461 tccaccaata tggaagagtc gaccgaaatc ctgacgattt ttccgtggta cagctacctg
    29521 gtcggcttat ttatttttgc gctcggcgta atagcaatca ggcgaaaaaa agagaatgaa
    29581 aaagcgcgct ggaatacctt cgacagcctg tgtctggtat tcagtgtggc gacatttttc
    29641 gttgctcccg tgcaaaacct ggcctggggt ggcgtattta aactgaaaga tactggctat
    29701 ccggtatttc gttttgctaa ggatgtcatc gtcaataata acgaggtgat tgaagagcaa
    29761 gaacggatgg caaaactttc cggaatgaaa gatacctgga cggtcactgc cgttaagccg
    29821 aagtatcaga cctatgtggt agtgatcggt gaaagcgcgc gtcgcgatgc cctcggtgcc
    29881 tttggcggtc actgggacaa taccccgttt gccagcagcg ttaacggttt gatatttgct
    29941 gactacattg ctgccagtgg ctccacgcag aaatcgcttg gcttaacgct caatcgcgta
    30001 gtcgatggca aaccacagtt tcaggataac tttgtcaccc tggcaaatcg cgcgggcttc
    30061 cagacctggt ggttttccaa ccagggccaa atcggcgaat acgatactgc tatcgccagc
    30121 atcgccaaac gtgcagatga agtgtacttc ctgaaagaag gtaattttga agcagataaa
    30181 aacacgaaag acgaagcgtt actggatatg accgctcaag tgctggcgca agagcactcg
    30241 caaccgcagc tgattgttct gcatctgatg ggctcgcatc cgcaggcctg cgacaggaca
    30301 caaggcaaat acgaaacctt tgtgcaatcg aaagaaacgt cgtgctatct ctataccatg
    30361 acgcaaacgg acgatttact gcgcaagctg tacgatcagt tacgcaacag cggcagcagc
    30421 ttctctctgg tttacttttc tgaccacggt ctggccttta aagagcgcgg caaagacgtg
    30481 caataccttg cccatgatga taaatatcag caaaatttcc aggtgccttt tatggtcatt
    30541 tccagcggcg ataaagcgca tcgggtgatt aaagcccgcc gctcagccaa tgacttctta
    30601 ggctttttct cgcagtggac gggaattaaa gcgaaggaaa taaatatcaa atacccgttt
    30661 atatctgaga agaaagccgg gccgatatac atcaccaact tccagttaca gaaggtggat
    30721 tacaaccatc tcggaaccga tattttcgac ccgaaacctt aaaaacaaaa atccgccccg
    30781 agaggcggat ttttatatca ccaaagtgat tagaagcggt aaccaacacc ggcaatccag
    30841 gtgcctacgt caacgctacg aatacggctc tgctcgtaag agaagtccag agcaacgttt
    30901 tccatcgggt tgaactgcag accagcaccg taggagaaac cgtagtcgct ggtgtcgtgt
    30961 ttgtaggtcg ggtattcagt ggtctggaat ttaccataac ccacacccac tacaccgtag
    31021 atgcttgccc agtcgttaat gcggtaagcc ggaccagcag tgatgccgta gtactggttt
    31081 ttgttgtagt caccagagct tgcagtacgg cttttctcgg tgtaagtgaa agaaccgatc
    31141 acacccagcg ggctgttgtc ttcttcatag cggtatttca ggttgaaacc gcccattttg
    31201 ttcatttggc cctgagcgtc gctctgtgcg taaccgccag ttacggtaga agtcgcagct
    31261 acggaagtac ctgcggtgaa agccagaact gcggccagtg ctgaaagaca tgcaattttt
    31321 ttcataacca cctcaaatgt gattcaaata agtcctaagt tttaaatata tcaaaaatta
    31381 atgggaaact cttcgcgatt tgtgatgtct aacgggccat ttcatgtaac agaacgtttc
    31441 catacaccgc tatccatcta aatttaaatc actttttcag agaactgcgt aagtattacg
    31501 catgttttcc ctgtcattca tccagattat tcctaatcac cagactaatg attccatcaa
    31561 tcctggcgca ttttagtcaa aacgggggaa aattttttca acaaatgctc aaccagcatt
    31621 gggtatatcc agtacactcc acgctttact taagtctaga tatttgtggg agaaaggatg
    31681 cctggttcat tacgtaaaat gccggtctgg ttaccaatag tcatattgct cgttgccatg
    31741 gcgtctattc agggtggagc ctcgttagct aagtcacttt ttcctctggt gagcgcaccg
    31801 ggtgtcactg cgctgcgtct ggcattagga acgctgatcc tcatcgcgtt ctttaagcca
    31861 tggcgactgc gctttgccaa agagcaacgg ttaccgctgt tgttttacgg cgtttcgctg
    31921 ggtgggatga attatctttt ttatctttct attcagacag taccgctggg tattgcggtg
    31981 gcgctggagt tcaccggacc actggcggtg gcgctgttct cttctcgtcg cccggtagat
    32041 ttcgtctggg ttgtgctggc ggttcttggt ctgtggttcc tgctaccgct ggggcaagac
    32101 gtttcccatg tcgatttaac cggctgtgcg ctggcactgg gggccggggc ttgttgggct
    32161 atttacattt taagtgggca acgcgcagga gcggaacatg gccctgcgac ggtagcaatt
    32221 ggttcgttga ttgcagcgtt aattttcgtg ccaattggag cgcttcaggc tggtgaagca
    32281 ctctggcact ggtcggttat tccattgggt ctggctgtcg ttattctctc gaccgctctg
    32341 ccttattcgc tggaaatgat tgccctcacc cgtttgccaa cacggacatt tggtacgctg
    32401 atgagcatgg aaccggcgct ggctgccgtt tccgggatga ttttcctcgg agaaacactg
    32461 acacccatac agctgctggc gctcggcgct atcatcgccg cttcaatggg gtctacgctg
    32521 acagtacgca aagagagcaa aataaaagaa ttagacatta attaaattta catttctgta
    32581 tggttatgca taaccatgca gaatttctcg ctacttttcc tctacaccgt atttatatat
    32641 cgaattatgc aaaagcatat ttattccgaa aattcctggc gagcagataa ataagaattg
    32701 ttcttatcaa tatatctaac tcattgaatc tttattagtt ttgtttttca tgcttgttac
    32761 cactattagt gtgataggaa cagccagaat agcggaacac atagccggtg ctatacttag
    32821 tctcgttaat tactgggaca taacatcaag aggatatgaa attatgagta ccgctaaatt
    32881 agttaaatca aaagcgacca atctgcttta tacccgcaac gatgtctccg acagcgagaa
    32941 aaaagcaaca gtagagttgc tgaatcgcca ggttatccag tttattgatc tttctttgat
    33001 taccaaacaa gcgcactgga acatgcgcgg cgctaacttc attgccgtac atgaaatgct
    33061 ggatggcttc cgcaccgcac tgatcgatca tctggatacc atggcagaac gtgccgtgca
    33121 gctgggcggt gtagctctgg ggaccactca agttatcaac agcaaaaccc cgctgaaaag
    33181 ttacccgctg gacatccaca acgttcagga tcacctgaaa gaactggctg accgttacgc
    33241 aatcgtcgct aatgacgtac gcaaagcgat tggcgaagcg aaagatgacg acaccgcaga
    33301 tatcctgacc gccgcgtctc gcgacctgga taaattcctg tggtttatcg agtctaacat
    33361 cgaataaatc catcgctgat ggtgcagaac tttagtaccc gataaaagcg gcttcctgac
    33421 aggaggccgt tttgttttgc agcccacctc aacgcactta tttagtgcat ccatctgcta
    33481 tccccagctg attaagtaaa ttttttgtat ccacatcatc acacaatcgt tacataaaga
    33541 ttgttttttc atcaggtttt acgctaaata atcactgtgt tgagtgcaca attttagcgc
    33601 accagattgg tgccccagaa tggtgcatct tcagggtatt gccctataaa tcgtgcatca
    33661 cgtttttgcc gcatctcgaa aaatcaatga gttgcaaaat tggcacgatt ttttcatata
    33721 tgtgaatgtc acgcagggga tcgtcccgtg gatagaaaaa aggaaatgct atgaagtctg
    33781 tattaaaagt ttcactggct gcactgaccc tggcttttgc ggtttcttct catgccgcgg
    33841 ataaaaaatt agttgtcgcg acggataccg ccttcgttcc gtttgaattt aaacagggcg
    33901 ataaatatgt gggctttgac gttgatctgt gggctgccat cgctaaagag ctgaagctgg
    33961 attacgaact gaagccgatg gatttcagtg ggatcattcc ggcactgcaa accaaaaacg
    34021 tcgatctggc gctggcgggc attaccatca ccgacgagcg taaaaaagcg atcgatttct
    34081 ctgacggcta ctacaaaagc ggcctgttag tgatggtgaa agctaacaat aacgatgtga
    34141 aaagcgtgaa agatctcgac gggaaagtgg ttgctgtgaa gagcggtact ggctccgttg
    34201 attacgcgaa agcaaacatc aaaactaaag atctgcgtca gttcccgaac atcgataacg
    34261 cctatatgga actgggcacc aaccgcgcag acgccgttct gcacgatacg ccaaacattc
    34321 tgtacttcat caaaaccgcc ggtaacggtc agttcaaagc ggtaggtgac tctctggaag
    34381 cgcagcaata cggtattgcg ttcccgaaag gtagcgacga gctgcgtgac aaagtcaacg
    34441 gcgcgttgaa aaccctgcgc gagaacggaa cttacaacga aatctacaaa aaatggttca
    34501 gtactgaacc gaaataataa cgctacacct gtaaagcgca ctggcagttc cctctccccc
    34561 cctggggaga ggattagggt gaggggcgca aacccgctcc ggggccatta attaccctga
    34621 atttgattat ttacaccacg gtaacaggaa caacatatgc agtttgactg gagtgccatc
    34681 tggcctgcca ttccgcttct gattgaaggt gccaaaatga ccctgtggat ttcggtcctc
    34741 ggtctggctg gcggtctggt aatcggattg ctggcaggtt ttgcacgcac cttcggtggt
    34801 tggatagcca accacgtcgc gctggtcttt attgaagtga tccgcggcac acctatcgtc
    34861 gtccaggtga tgtttatcta tttcgccctg ccgatggcgt ttaacgactt acgcatcgac
    34921 ccatttactg cggcggtggt caccatcatg atcaactccg gcgcgtacat tgcggaaatc
    34981 acgcgtggtg cggtgctgtc tatccacaaa ggttttcgtg aagcaggact ggcgctcggt
    35041 ctttcacgtt gggaaaccat tcgctacgtc attttaccgc tggcactgcg tcgtatgctg
    35101 ccgccgctgg gtaaccagtg gatcatcagc attaaagaca cctcgctgtt tattgttatc
    35161 ggcgtggcgg aactgacccg tcaggggcaa gaaattattg ccggtaactt ccgcgccctt
    35221 gagatctgga gcgccgtggc ggtgttctat ctgattatta ccctggtgct gagctttatt
    35281 ctgcgtcgtc tggaaagaag gatgaaaatc ctgtgattga atttaaaaac gtctccaagc
    35341 actttggccc aacccaggtg ctgcacaata tcgatttgaa cattgcccag ggcgaagtcg
    35401 tggtgattat cgggccgtcc ggttccggta aatcgaccct gctgcgctgc atcaacaaac
    35461 tggaagaaat cacctccggc gatctgattg tcgatggcct gaaggtcaac gatccgaaag
    35521 ttgacgagcg cctgattcgc caggaagcag gtatggtgtt ccagcagttt tacctcttcc
    35581 cgcatctgac ggcgctggga aacgtcatgt ttggtccgct acgcgtgcgt ggcgcgaaca
    35641 aagaagaggc ggaaaaactg gcgcgtgagc tgctggcgaa agtcggtctg gcagaacgtg
    35701 cacatcacta cccttccgaa ctttctggtg gtcaacagca gcgtgtggcg attgcccgcg
    35761 cgctggcggt gaagccgaaa atgatgctgt ttgatgaacc gacttccgct cttgacccgg
    35821 aactgcgcca tgaagtgctg aaggttatgc aggatctggc tgaagaaggg atgacgatgg
    35881 tgatcgtgac ccacgaaatc ggttttgccg agaaagtagc ttcgcggctg atctttatcg
    35941 acaaaggccg gattgcggaa gatggcaatc cgcaggtgtt gatcaagaac ccgccgagcc
    36001 agcgcttgca ggaatttttg cagcacgtct cttaataaga cacattgcct gatgcgctac
    36061 gcttatcagg cctacaggat atctggcaac ttattaaaat tgcatgaact tgtaggacgg
    36121 ataaggcgtt cacgccgcat ccggcaaaaa agcccgcacg ttgtcagcaa cctgcttaat
    36181 atcccttcct ccctttcacc cgaaagggag gcacaccaga ttcctctcat ttaaaatcgc
    36241 ccctccccca gcatctatac ttatcttttt gctctgtttt ctcactggag gagtcatgcg
    36301 gtggatcctg ttcatcctct tctgcctgct gggcgcacct gcccacgcgg tatccatacc
    36361 cggcgttaca accacaacga caacggactc aacgactgaa ccggccccgg aaccggatat
    36421 cgaacaaaaa aagcggccta tggcgcactg gcggatgtgc tggataacga cacctcgcgt
    36481 aaagagttga tcgaccagtt gcgtactgtc gccgcgacgc cccctgcgga accggtcccg
    36541 aagatcgtgc cgccgacgct ggttgaagag caaaccgtgc tgcaaaaggt caccgaagtc
    36601 agccgccatt atggtgaagc cctttccgcc cgcttcgggc aactttatcg caatatcacc
    36661 ggctccccgc ataagccatt taatccacaa accttcagca atgcgctgag ccatttttca
    36721 atgttagcgg tattagtgtt tggtttttac tggctgattc gcctgtgcgc actgccgcta
    36781 tatcgcaaaa tgggtcagtg ggcgaggcaa aaaaatcgtg agcgcagcaa ctggttgcag
    36841 cttccggcga tgattattgg ggcgtttatt atcgacctgc tgttactggc cctgacatta
    36901 tttgtcggcc aggtattaag cgacaacctg aatgcaggca gtcgcaccat cgctttccaa
    36961 caaagtttgt ttctcaacgc ctttgccctg attgaatttt tcaaagccgt actacgcctg
    37021 attttttgcc caaacgtggc ggagctgcgc ccgttcacga ttcaggacga gagcgcccgt
    37081 tactggggtc gtcgcctgag ctggttaagc agcctgattg gctatggcct gattgtggcc
    37141 gtgccgatta tctctaatca ggtgaatgta cagataggtg cgctggcgaa cgtcatcatt
    37201 atgctgtgca tgaccgtctg ggcgttgtac ctgatcttcc gaaataaaaa agagattacc
    37261 cagcatatgc tcaactttgc ggagcattcg ctggcttttt tcagcctgtt tatccgcgcc
    37321 tttgcgctgg tgtggcactg gctggcaagc gcctatttta tcatgctgtt tttcttttcg
    37381 ttgttcgatc cgggcaacag tctgaaattt atgatgggtg caacggtgcg cagcctggcg
    37441 attattggta tcgcagcgtt tgtttccggt atgttttccc gctggctggc gaaaaccatc
    37501 accctctcgc cacatactca gcgtaactat ccggagctac aaaaacggct gaatggctgg
    37561 ctttcggcgg cgctgaaaac ggcgcgtatt ctgaccgtct gcgtggcagt aatgttgctg
    37621 ttgagcgcat ggggattgtt cgatttctgg aactggctgc aaaacggcgc ggggcagaaa
    37681 accgtagata tcctgatccg catcgcactc attcttttct tctcggcggt tggctggacg
    37741 gtgctcgcca gtttgatcga aaaccggctg gcttcggata ttcatggccg cccgctaccc
    37801 agcgcccgta cgcgtaccct gctgacgctg ttccgtaacg cgctggcggt gattatcagt
    37861 accatcacca tcatgattgt gttgtcggaa atcggcgtca atatcgcgcc attgctggca
    37921 ggtgccgggg cattaggtct ggctatctcg tttggttcgc aaacgctggt aaaagatatt
    37981 atcaccgggg tatttattca gtttgaaaac ggcatgaaca ctggagattt ggtgactatc
    38041 gggccgttga ccggcacagt ggaacggatg tcgattcgct ccgtgggcgt gcgccaggat
    38101 accggggcgt atcacatcat tccgtggtct tcgataacca cctttgctaa cttcgtccgc
    38161 ggtattggtt cggtagtggc aaactacgat gttgatcgcc atgaagatgc tgataaagcc
    38221 aatcaggcac tgaaagatgc ggtaacggaa ttgatggaaa acgaagaaat tcgcgggctg
    38281 attattggtg aaccgaactt tgccgggatt gtcggcttaa gcaataccgc gtttacactg
    38341 cgtgtttcgt tcaccacgct gccactcaaa cagtggacgg tacgctttgc cctcgacagc
    38401 caggtgaaaa aacatttcga cctggcgggc gttcgcgcgc cagtgcagac ttatcaggtg
    38461 ctgcctgctc cgggcgcgac cccggctgaa ccgctgccgc cgggggagcc aacgctttaa
    38521 cgttggcgat taacaaaacg gcggcgctgc tcgtcgttca taaaggtcca ggcaataaag
    38581 cgactctgct tttgcccctg ggccatctct ttttttacca ccttcaccgc gcccacgtcg
    38641 gtcagagcac gatacaacgg cggtaagttt tcaccacgag ataccagcga ggtaaaccac
    38701 atcacctgct tcgcgaagcc tttgctctct tcaatcatct ttttgataaa gttgacttca
    38761 ccgccttcac accacaactc ctgttgctga ccaccaaagt tcagtgcatc gtctttgttc
    38821 agccccaggt tacggcgttt acgctcacta cctgcccgcg ccgcagcggc ggaatcgtgg
    38881 aatggcgggt tacacaaggt cgcgtcgtat tgctcgtttt tgtggatgat gccgttaaaa
    38941 atcgccccac tctctttttg ccgacgcaga cgaatggcgc ggttaagccc cggattagca
    39001 ctgataatcg cctgcgcact gcttaacgcc tggctgctgg tttcactacc ggtaaaacgc
    39061 cagccatatt catgtacgcc aatcagcgga taaatacagt tcgcaccaac gccgatatcc
    39121 agaatgctgg cattcgctgg aattgttccg cttgcttcag ccagtaaatc ggcaaggtga
    39181 tgaatataat ccgcccggcc cggtactggt gggcagagaa aaccgtcggg gatatcccag
    39241 ttcgctacgg cgtaaaaatg ggccagcaac gccttattga gcgccttcac cgccagcgga
    39301 ttggcaaagt ctacgctttg ctcccctgcg ggtgtaagcg tgaggaattg cctgagttca
    39361 ggattgacct gacaaagcgt ggcgagatcg tagcggctgt gatgacggtt gcgcggatgc
    39421 aaccccggtt tctgggcgga catagcattc tcctttaagc agcggcgtaa gatacccgca
    39481 taacgcgaca gggtaaatat ctggctgcgt ttatcattcc ccaaaacctt aacatggcta
    39541 tggtttatgg caattcatca taaaaatcag cattatgcat gtcattgatt tgtcttattt
    39601 tgtaaaaata tatcgttcaa aaaataagca tttctctgtc aattttttgc gtcatttgca
    39661 tcgcgcaggc gacctacact tatcaggcac tacccgttgt atttatgagg atgtcattat
    39721 gaaaaagtgc ctcacactac tgattgccac cgtcctgagc ggaatctctc tcacggctta
    39781 tgccgcgcaa ccgatgagta acctggacag cggtcagcta cggcccgccg gtaccgtttc
    39841 ggcaactggc gcatcaaacc taagcgatct ggaggataaa ctggcagaaa aagcgcgcga
    39901 acaaggcgcg aaaggttatg tcattaattc cgcaggcgga aatgaccaga tgttaggtac
    39961 tgcaaccatc tacaaataac cccaccaacg taactgcatt cgcctctgga tgcagttttc
    40021 gcctgttttg taatcaaaaa acatcaaatg tgaacgcaat gtaaataaat gtatttcttt
    40081 ttcgcgcaat gggtgataga aaatcgcatc aagtgataat gcttatcaaa attattatca
    40141 cttgcacgaa cactatcacg ggattaacag tggcatcgca tccgcagaga tgctttctcg
    40201 tggcagtgaa aatttcaaca tataagaaaa agtcacctgc aaaatggaaa acaatcgcaa
    40261 tttccctgcc agacaatttc attcgctcac gttctttgcc ggtctttgta ttggtattac
    40321 gcctgtggca caggcactcg ccgccgaagg gcaagctaac gcggatgaca cgctggttgt
    40381 cgaagcatca acgccttcgc tttatgcgcc acaacaatct gccgatccga aattctcgcg
    40441 tccggtagcg gatactaccc gcacgatgac ggtgatttct gaacaagtga ttaaagatca
    40501 gggcgcaacc aaccttaccg acgcgctcaa aaacgtcccc ggcg
//
LOCUS       DAEBXN010000029        37857 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.029, whole genome shotgun sequence.
ACCESSION   DAEBXN010000029 DAEBXN010000000
VERSION     DAEBXN010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37857
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.029"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(<1..74)
                     /locus_tag="KJL99_001526"
     CDS             complement(<1..74)
                     /locus_tag="KJL99_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="MliC; membrane-bound lysozyme inhibitor of c-type
                     lysozyme; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor"
                     /protein_id="HBD4436300.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQLV"
     gene            complement(172..1281)
                     /gene="anmK"
                     /locus_tag="KJL99_001527"
     CDS             complement(172..1281)
                     /gene="anmK"
                     /locus_tag="KJL99_001527"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HBD4436301.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQLVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAVLLLGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            1555..2022
                     /gene="slyB"
                     /locus_tag="KJL99_001528"
     CDS             1555..2022
                     /gene="slyB"
                     /locus_tag="KJL99_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HBD4436302.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(2069..2503)
                     /gene="slyA"
                     /locus_tag="KJL99_001529"
     CDS             complement(2069..2503)
                     /gene="slyA"
                     /locus_tag="KJL99_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HBD4436303.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            2704..2940
                     /locus_tag="KJL99_001530"
     CDS             2704..2940
                     /locus_tag="KJL99_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HBD4436304.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            2943..3800
                     /locus_tag="KJL99_001531"
     CDS             2943..3800
                     /locus_tag="KJL99_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBD4436305.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGELLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFIFAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQLVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            3800..5812
                     /locus_tag="KJL99_001532"
                     /pseudo
     CDS             3800..5812
                     /locus_tag="KJL99_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(5813..6334)
                     /gene="sodC"
                     /locus_tag="KJL99_001533"
     CDS             complement(5813..6334)
                     /gene="sodC"
                     /locus_tag="KJL99_001533"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HBD4436306.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(6415..7311)
                     /locus_tag="KJL99_001534"
     CDS             complement(6415..7311)
                     /locus_tag="KJL99_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HBD4436307.1"
                     /translation="MVQRITIAPEGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RNHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(7360..7599)
                     /locus_tag="KJL99_001535"
     CDS             complement(7360..7599)
                     /locus_tag="KJL99_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HBD4436308.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            7702..8301
                     /gene="nemR"
                     /locus_tag="KJL99_001536"
     CDS             7702..8301
                     /gene="nemR"
                     /locus_tag="KJL99_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HBD4436309.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLAELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            8338..9435
                     /gene="nemA"
                     /locus_tag="KJL99_001537"
     CDS             8338..9435
                     /gene="nemA"
                     /locus_tag="KJL99_001537"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HBD4436310.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMALQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAAFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQLKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            9516..9923
                     /gene="gloA"
                     /locus_tag="KJL99_001538"
     CDS             9516..9923
                     /gene="gloA"
                     /locus_tag="KJL99_001538"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HBD4436311.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            10026..10673
                     /gene="rnt"
                     /locus_tag="KJL99_001539"
     CDS             10026..10673
                     /gene="rnt"
                     /locus_tag="KJL99_001539"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HBD4436312.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            10766..12880
                     /locus_tag="KJL99_001540"
     CDS             10766..12880
                     /locus_tag="KJL99_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBD4436313.1"
                     /translation="MADNPDPSSLLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARG
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQIPLKGIADERRRRGETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGL
                     SATVRSASDVAAFLGGDRPVTVVNPPTMRHPQIRIVVPVANMDDVSSVASGTGEDSHA
                     GREGSIWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVD
                     AAHFESTSGATSNRVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLEL
                     GIDMGAVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVEC
                     MFAGRLENLTPPHNPLDVLAQQTVAAAAMDALQVDEWYSRVRRTAPWKDLPRRVFDAT
                     LDMLSGRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPE
                     GEEKAGSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSAQLPF
                     WRGEGNGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTNANIQGLIDEQRNATG
                     IVPGSRHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASD
                     DSIVARIPDTDGKLPDAAIFLF"
     gene            12890..14182
                     /locus_tag="KJL99_001541"
     CDS             12890..14182
                     /locus_tag="KJL99_001541"
                     /inference="COORDINATES: protein motif:HMM:NF020083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4436314.1"
                     /translation="MLQIVREAVGSSALFAARFRECAARALLMPGRTPGHRTPLWQQR
                     LRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQISDVTTTTP
                     SPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELLDPQVIRQ
                     VEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEVASYLENLLAVKR
                     IFPAMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLRYLRAHAL
                     VTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRSLQAAREA
                     TRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQLAGVGLPA
                     SLWESQILPARVRDYSPEMLDELLATGAVIWSGQKKAG"
     gene            15132..15224
                     /locus_tag="KJL99_001542"
     CDS             15132..15224
                     /locus_tag="KJL99_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001441976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBD4436315.1"
                     /translation="MNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(15268..16476)
                     /locus_tag="KJL99_001543"
     CDS             complement(15268..16476)
                     /locus_tag="KJL99_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBD4436316.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFQKRAAFPR
                     FKKRGQNAAFRYPQDVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWY
                     ISIQTESEVSTPVHPSASMIGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWSGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQ
                     EPTEMIQATA"
     gene            16484..16984
                     /gene="tnpA"
                     /locus_tag="KJL99_001544"
     CDS             16484..16984
                     /gene="tnpA"
                     /locus_tag="KJL99_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HBD4436317.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTKYRRQIFDHDTTEK
                     LRTYFSNVCADFAAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGISNRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCEGAPISVIRQYIEQQQTPGQVENRALYPRPEGRGFT
                     AHRIKD"
     gene            complement(17023..17370)
                     /gene="grxD"
                     /locus_tag="KJL99_001545"
     CDS             complement(17023..17370)
                     /gene="grxD"
                     /locus_tag="KJL99_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HBD4436318.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            17705..18532
                     /gene="mepH"
                     /locus_tag="KJL99_001546"
     CDS             17705..18532
                     /gene="mepH"
                     /locus_tag="KJL99_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HBD4436319.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSMTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAGPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            18660..19241
                     /gene="sodB"
                     /locus_tag="KJL99_001547"
     CDS             18660..19241
                     /gene="sodB"
                     /locus_tag="KJL99_001547"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HBD4436320.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19387..20556)
                     /locus_tag="KJL99_001548"
     CDS             complement(19387..20556)
                     /locus_tag="KJL99_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD4436321.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMHVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVTGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(20722..20811)
                     /gene="ynhF"
                     /locus_tag="KJL99_001549"
     CDS             complement(20722..20811)
                     /gene="ynhF"
                     /locus_tag="KJL99_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HBD4436322.1"
                     /translation="MSTDLKFSLVTTVIVLGLIVAVGLTAALH"
     gene            21110..22135
                     /gene="purR"
                     /locus_tag="KJL99_001550"
     CDS             21110..22135
                     /gene="purR"
                     /locus_tag="KJL99_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HBD4436323.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(22132..23064)
                     /locus_tag="KJL99_001551"
     CDS             complement(22132..23064)
                     /locus_tag="KJL99_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBD4436324.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLSDSETLNKEWLREPEETPATGD"
     gene            23177..24388
                     /locus_tag="KJL99_001552"
     CDS             23177..24388
                     /locus_tag="KJL99_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HBD4436325.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCPNH
                     GNAEVAHSESH"
     gene            24679..25827
                     /gene="cfa"
                     /locus_tag="KJL99_001553"
     CDS             24679..25827
                     /gene="cfa"
                     /locus_tag="KJL99_001553"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HBD4436326.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCKGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(25867..26508)
                     /gene="ribE"
                     /locus_tag="KJL99_001554"
     CDS             complement(25867..26508)
                     /gene="ribE"
                     /locus_tag="KJL99_001554"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HBD4436327.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            26723..28096
                     /gene="mdtK"
                     /locus_tag="KJL99_001555"
     CDS             26723..28096
                     /gene="mdtK"
                     /locus_tag="KJL99_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HBD4436328.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRYMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPEPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(28137..29393)
                     /locus_tag="KJL99_001556"
     CDS             complement(28137..29393)
                     /locus_tag="KJL99_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HBD4436329.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVLGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            29701..29777
                     /locus_tag="KJL99_001557"
     tRNA            29701..29777
                     /locus_tag="KJL99_001557"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29735..29737,aa:Val,seq:gac)
     gene            29782..29858
                     /locus_tag="KJL99_001558"
     tRNA            29782..29858
                     /locus_tag="KJL99_001558"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29816..29818,aa:Val,seq:gac)
     gene            29966..30271
                     /locus_tag="KJL99_001559"
     CDS             29966..30271
                     /locus_tag="KJL99_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HBD4436330.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            30397..31819
                     /locus_tag="KJL99_001560"
                     /pseudo
     CDS             30397..31819
                     /locus_tag="KJL99_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
     gene            complement(31831..32643)
                     /locus_tag="KJL99_001561"
     CDS             complement(31831..32643)
                     /locus_tag="KJL99_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4436331.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIRHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGLGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(32647..33432)
                     /locus_tag="KJL99_001562"
     CDS             complement(32647..33432)
                     /locus_tag="KJL99_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HBD4436332.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(33429..34097)
                     /locus_tag="KJL99_001563"
     CDS             complement(33429..34097)
                     /locus_tag="KJL99_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBD4436333.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYLVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(34161..34799)
                     /locus_tag="KJL99_001564"
     CDS             complement(34161..34799)
                     /locus_tag="KJL99_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HBD4436334.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(34812..36913)
                     /locus_tag="KJL99_001565"
                     /pseudo
     CDS             complement(34812..36913)
                     /locus_tag="KJL99_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
     gene            complement(36934..37560)
                     /locus_tag="KJL99_001566"
     CDS             complement(36934..37560)
                     /locus_tag="KJL99_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HBD4436335.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
BASE COUNT         9100 a         9897 c         9302 g         9558 t
ORIGIN      
        1 accagttgat taaaggcgct acaaccggag agaaggaccg gcaaaatgat aattaacagt
       61 tttttcatag tcatatcccg aagactttcc tggtccggag ggcaaatacc ctccttaatc
      121 ttccaagtgt aacggcagac acgataagaa aaaattcagt taattctgat atcacgagtt
      181 ggcggggaaa atagccccca gtaccgtctc ctggcttgcg ccagtgacgg aaggcagatt
      241 tcctggtaat cccgccagcg tccgccaggc aagccaggcg aaagccaatg cttccatgtc
      301 atcgccacta atgccaacgg catcggtggt ggtgacttct gtgcctagca gtaataccgc
      361 cagacgcgcc atgagtagcg gattacgact acctccacca cataccatca atcgttcgca
      421 gccaccgctc aacaaaactt gttcagaaat ggtcacggcg gtgagttctg ccagtgtggc
      481 ctgaacatca cggggatcaa cacccggaaa atggcgtaaa tggcgctcca gccagccgta
      541 gttaaagtat tcgcgtccgg tgcttttcgg tgcaggttgc gagaaatacg ggtcgctgag
      601 catattttgc agcagtggga gaataacttt acccgcccgt gcccactcgg catctttatc
      661 gtaaggttta cctgcctgac gccagatcca ggcatccatc agcatgttac caggaccggt
      721 atcgtagccg ccaaccagct gcccaggaat gagcagtgac agattggcga tgccgccaat
      781 attgagcacc attcgtcgct cggttgggtg agccagcaag gcatgatgga acgcaggtac
      841 cagcggtgcg ccttgtcctc ccaaggcaat atcgcgacgg cgaaaatcac cgacaaccgt
      901 aattccggtg cgtgccacaa tttgattgtt atcgccaatc tgtaaagtgt gtggtgccac
      961 gcccgtcggt tcatgccaga cggtttgacc gtgacagccg atcgcaacta tatctctcgc
     1021 ctgcaggttt tgttccttaa gcaaggcatt gaccgcatca gcaaaaagtc gcccgagttg
     1081 agtatcaagc tgtccaaact gcgaaagtgt aagctgctgg ccctggcaaa tatccagtac
     1141 agcctgtttc agagacaccg ggatcggcca gctcaagctt gccagctgtg cgacccggtg
     1201 ttcatcaatt gtcgccaaca caacatcaac accatcaagg ctggtgcctg acataacgcc
     1261 aataaagcgg cccgatttca tagttcatcc tttttcaatc tgacgtttgc gcaccactca
     1321 aacataaact tttcgtgaat accatgcgga atgaccgatt tttaccgttg gtagtaaaac
     1381 attatcttca aatcaataat catcatgaat gttttgttta taattggttg atcctacttt
     1441 cattatgatt tgctcatatt tggtagaaca tgtaaccatg gattcacata tgccatatac
     1501 tttgaccatg agggatgctt gcgtggcgtt tcatggtgaa caggagattt ttcaatgatt
     1561 aaacgcgtat tggttgtttc aatggtaggt ctgtctcttg tcggttgtgt taataacgac
     1621 accctgtcag gggatgttta taccgcttct gaagcgaaac aagtacagaa tgtcagctat
     1681 ggcaccatcg ttaacgtacg tccggtacag attcagggcg gtgatgattc caacgttatc
     1741 ggtgcaattg gcggtgctgt tcttggtggt ttcctgggga atactgttgg tggcggaacc
     1801 gggcgttctc tggctactgc agcaggcgct gttgcaggtg gcgtagctgg tcagggcgta
     1861 cagagtgcaa tgaacaaaac gcagggtgtc gagctggaaa ttcgtaaaga cgatggtaat
     1921 accatcatgg tggtacagaa acaaggcaac actcgtttct ctccgggcca acgtgtcgta
     1981 ctggccagca atggcagtca ggtgaccgtt tctccgcgct aaataagttt gcgtgtggtc
     2041 aggttactga ccacacgccc ccttcatttc accctttggc ctgtaactca atgatattat
     2101 gctcaagttt tgcgatgagc gtaatcagtt gctccagttc ctctgcggag atgccatgta
     2161 atatttccgc gcgggttttg ttaataacag cttccatttc gctgatcagt ggctctgcct
     2221 tttccgtcag tttaatacgt ttagcccgac gatcgctggc acaagtttga cgcgaaatta
     2281 accctttttc ttccagttgg tccagagtac ggaccagtga tggctgctcg atgccaatcg
     2341 cttttgccag ttgaatttgc gactggtctg gaggtaactg atggatattg tgtaacgtaa
     2401 cccaatgggt ttgtgttaac tccagcggtt tcaggcgatg gtctatcaga gcacgccata
     2461 tgcgcaccaa ccgtgccaga tcagaaccta gtggcgattc caatttcatc tccttataat
     2521 tagcttgcta agatattatg cggcttttag aatagtgtgc agcaattgta ttgctaaaac
     2581 aaatgtattg ctgcatttgg ttaccgtcag acatattttt cagaaattgc gcgtaaattt
     2641 ttcgcactta aagaatattt attaatctaa cgcaatatat tcggtcgtaa aggaatctac
     2701 tttgtgaagt ttatgctcaa tgcaacagga ttgcccttac aagatctggt gttcggtgcg
     2761 tccgtctatt ttcctccgtt tttcaaagca ttcgcgtttg gattcgtcat ctggcttgtc
     2821 gtacaccgcc tgcttcgtgg ctggatctac gccggcgata tctggcatcc cttgttaatg
     2881 gatttatcgc tgtttgcgat ttgcgtttgc cttgctctgg caatactgat tgcgtggtaa
     2941 ctatgtcaat taaaacaatt aagtatttct caacaatcat tgtagcgata gttgcggttc
     3001 ttgccggatg gtggctgtgg aattattaca tgcaatctcc gtggacacgc gacgggaaaa
     3061 tacgcgcaga acaggtttct atcaccccac aggtgtcggg acgtattgtt gagctgaata
     3121 taaaagacaa ccaactggtt aacgcggggg aacttctgct cacgatcgat aaaacgccct
     3181 ttcagatcgc cgagctgaac gcacaggctc agctggcgaa agcgcaatct gacctcgcaa
     3241 aagccaataa cgaagcgaat cgtcgtcgtc atctctcaca aaattttatt tttgccgaag
     3301 aactcgatac cgctaacctc aatgttaaag cgatgcaggc cagtgttaat gccgcacagg
     3361 cgacgctgaa acagacacaa tggcaactgg cgcaaacgga aattcgcgcc ccggtgagtg
     3421 gatgggtgac taacctcact acccgcatcg gcgattacgc tgacaccggg aaaccgctgt
     3481 ttgcccttgt cgacagccac tcgttttacg tcattggtta ttttgaagaa accaagttgc
     3541 gccatatccg cgaaggtgca ccagcacaaa ttaccctcta tagtgataac aaaacgttac
     3601 agggtcacgt ttccagtatc ggtcgggcga tttatgatca gagcgttgaa agtgactcaa
     3661 gccttattcc ggatgtgaaa cctaacgtcc cctgggttcg tctcgcccaa ctcgttcccg
     3721 tccgctttgc gctggataaa gttccaggcg atgtcacgct ggtgtcgggc accacctgta
     3781 gcattgccgt aggtcaataa tgaacgcatc gtcatggtcc ttgcgcaatt tgccctggtt
     3841 cagggccacg ctggcgcaat ggcgttatgc gttacgcaat accattgcca tgtgtctggc
     3901 gctgacggtt gcctattatt tgaatctgga tgaaccctat tgggcgatga cctcggctgc
     3961 agtggttagc tttcccaccg ttggcggtgt catcagcaaa agcctcggac gcatggctgg
     4021 cagtttactg ggagccattg cggcactgct tcttgccgga catacgctca atgagccgtg
     4081 gttttttcta ttgagcatgt cggcgtggct tggcttttgt acctaggcct gtgcgcactt
     4141 cacgaataac gtcgcgtatg catttcaact ggcgggctac acggctgcca tcatcgcctt
     4201 tccgatggtt aatattactg aggccagcca gctgtgggat atcgctcagg cgcgcgtttg
     4261 cgaggtgata gtcggtattt tgtgcggcgg catgatgatg atgatcctgc ctagcagttc
     4321 cgatgctact gcccttttaa ctgcattgaa aaacatgcac gcccgattac tggaacatgc
     4381 cagtttactc tggcagcctg aaacaaacga tgccattcgt gcagcacatg agggggtgat
     4441 tgggcagata ctgaccatga atttgctgcg tatccaggct ttctggagcc actatcgttt
     4501 tcgccagcaa aacgcgcgcc ttaatgcgct actccaccag caattacgta tgaccagtgt
     4561 catctccagc ctgcgacgta tgttgctcaa ctggccctca ccgccaggtg ccacgcgaga
     4621 aattctcgaa caattgctga cggcgctcgc cagttcgcaa acagatgctt acaccgtcgc
     4681 acgtattatc gccccgctac gcccgaccaa cgtcgccgac taccggcacg tcgccttctg
     4741 gcagcgacta cgttattttt gccgccttta tctgcaaagt agtcaggaat tacatcgtct
     4801 gcaaagcggt gtagatgatc atgccagact cgcacggaca tccgctctgg ctcgtcatac
     4861 cgacaacgcc gaagctatgt ggagcgggct gcgtacattt tgtacgttga tgatgattgg
     4921 cgcatggagt attgcatcgc aatgggatgc cggtgccaat gcattaacgc tggcagcaat
     4981 tagctgcgta ctctactccg cagtcgcagc accgtttaag tcgttgtcac tgctgatgcg
     5041 cacgctggtg ttactttcgc tattcagctt tgtggtcaaa tttggcctga tggtccagat
     5101 tagcgatctg tggcaatttt tactgtttct ctttccactg ctggcgacaa tgcagcttct
     5161 taaattgcag atgcccaaat ttgccgcact gtgggggcaa ctgattgttt ttatgggttc
     5221 gtttatcgct gtcactaatc ccccggtgta tgattttgct gattttctta actataatct
     5281 ggcaaaaatc gttggcgttg cgctggcttg gctagcgttc gccattctgc gtccaggatc
     5341 ggatgctcgt aaaagccgcc gccatattcg cgcgctgcgc cgggattttg tcgatcagct
     5401 aagccgccat ccaacactga gtgaaagcga atttgaatcg ctcacttatc atcacgtcag
     5461 tcagttgagt aacagccagg atgcgctggc tcgccgttgg ttattacgct ggggtgtagt
     5521 gctgctgaac tgttctcatg ttgtctggca attgcgcgac tgggaatcgc gttccgatcc
     5581 gttatcgcga gtacgggata actgtatttc actgttgcgg ggagtgatga gtgagcgtgg
     5641 cgttcagcaa aaatcactgg cggccacact tgaagaatta cagcggattt gcgacagcct
     5701 tgcccgtcat catcaacctg ccgcccgtga gctggcggca attgtctggc ggctgtactg
     5761 ctcgctttcg caacttgagc aagcaccacc gcaaggtacg ctggcctcgt aattacttaa
     5821 ttacaccaca ggcatagcgt tcaccgccac cgcccagcgg tttaggttga tcggacatat
     5881 tatcgccgcc aacgtggacc atcagcgcct tgtctttgat ttcatccagt gatttcagac
     5941 gaggcgcgat gacggcatcg gtagctttgc cgtcattatt gacgaccagt gcaggcagat
     6001 cgcctaaatg cccggtacct tctggccctt catgtttacc ggtattttgt ggatcaagat
     6061 gcccgcctgc ggattccgcg gcgctggctt tgccatcttt ggtggctggc tggcagcttc
     6121 ctttggcatg aatatggaag ccatgttcac cggggggtaa tgctttcaga tcgggtgaaa
     6181 actccagacc tttatcggtt tcagtaatgg tgacgctacc aattgactgc ccaacccctt
     6241 gcgacgtgac gaggttcatc tcgacttttt cactggcagc ttgtgcgccg gtcgcaacaa
     6301 ccagcgccag aatggccaga ctaaaacgtt tcataggacc tccgttcatt gtgcattcct
     6361 gaataagtgt aaaccagtga cacatttttg aacggcggct attcctaaaa gtgcttacgg
     6421 tacgtcgtac cccagtgcag ctttacggat acgaaaccat tgttgacggg tcattttcag
     6481 tgtttctgct tcgacagctg cccgtacgcg ctcaatttta cctgagccga taattggcag
     6541 cggctgcgac ggtaaacgta atacccaggc gtaaaccacc tgttcaatcg agcccgcgtt
     6601 taactcctcc gccaccacag ccagttcatc acgcagcggc tggaaataat catcattaaa
     6661 cagacgacca ccaccaaggc aggaccacgc catcggacga acacgcagtt gttgtagttg
     6721 gtcgagcgtg ccatccagca gtaacggctg atgcaccggg gatatttcca cctgattagt
     6781 ggcaagggta aacggcagac gtgattgcaa cagggcaaat tgcgcaggcg taaagttcga
     6841 tacgccaaaa tgacgcactt tgccgctctg atgcagatgt ttgaacgcgt ccgccacttc
     6901 atcggcatcc attaacgggt ctggtcggtg gattaacagc agatccagat gatcggtcgc
     6961 gagattaatt agcgactgtt cggcgctctt aatgatgtga ttgcggtcag tgatgtaatg
     7021 accaatgacg ttttcttcac gcgcggtcgt cgcgataccg catttactga cgatttccat
     7081 ccgttcacgc aggtgaggtg ccagtttcag tgcctcgcca aacgccgctt cgcactgata
     7141 gccaccataa atatcagcat ggtccacggt ggtcacgccg agatccagat gctcttcaat
     7201 aaaactgacc agctggcggg cggacatatt ccagtccatc aatcgccagt agcccatcac
     7261 aaaacgggaa aactccgggc cttccggcgc aatagtaata cgctgaacca taatcgcttc
     7321 ctcttatcag atataagagg agtatacgca agattaggtt caaaagagtg atggttgctc
     7381 cggttcgtct gatgacgctg gcttatttgc gcgtaatttg cgcattaatc gctgccgaca
     7441 aaggcgcagc acctcttgtt tttcgccatc gctcatttta ttccagttaa aacgctcatc
     7501 ccgactacga aaacagccgc gacaaaaccc gcgttcgtca gactggcaaa ttccccggca
     7561 cgggctctgg acgggaaaga actctaattg ctccgccacc tcgccctcct cagataagat
     7621 tattaccatt attgaagctg ttaatgtcca aagtagcaac tttgcttgca ctagaccaac
     7681 tggtctacta cactccaacg catgaacaaa cacaccgaac atgatactcg cgaacatctc
     7741 ttggcgacgg gcgagcaact ttgcctgcaa cgtggattca ccgggatggg gctaagcgaa
     7801 ttactaaaaa ccgctgaagt gccgaaaggg tccttctatc actactttcg ctctaaagaa
     7861 gcgtttggcg ttgccatgct tgagcgtcat tacgccgcat atcaccagcg actggctgag
     7921 ttgctgcaat ccggcgaagg taactaccgc gaccgcatac tggcttatta ccagcaaaca
     7981 ctgaaccagt tttgccaaca tggaaccatc agtggttgcc tgacagtaaa actctctgcc
     8041 gaagtgtgcg atctgtcaga agatatgcgc agcgcgatgg ataaaggtgc tcgcggcgtg
     8101 atcgccctgc tctcgcaggc tctggaaaat ggccgtgaga accattgttt aaccttttgt
     8161 ggcgaaccgc tacaacaggc acaagtgctt tacgcactat ggcttggcgc gaatctgcag
     8221 gccaaaattt cgcgcagttt cgagccactg gaaaacgcgc tggcccatgt aaaaaacatt
     8281 attgcgacgc ctgccgttta gcaggcattt tttatcacca gacgaccggg agcctttatg
     8341 tcatctgaaa aactgtattc cccactgaaa gtgggcgcga tcacggcggc aaaccgtatt
     8401 tttatggcac cgctgacgcg tctgcgcagt attgaaccgg gtgacattcc taccccgttg
     8461 atggcggaat actatcgcca acgtgccagt gccggtttga ttattagtga agccacgcaa
     8521 atttctgccc aggcaaaagg atatgcaggt gcgcctggca tccatagtcc ggagcaaatt
     8581 gccgcatgga aaaaaatcac cgctggcgtt catgctgaaa atggtcatat ggccttgcag
     8641 ctgtggcaca ccggacgcat ttctcacgcc agcctgcaac ctggcggtca ggcaccggta
     8701 gcgccttcag cacttagcgc gggaacacgt acttctctgc gcgatgaaaa tggtcaggcg
     8761 atccgcgttg aaacatccat gccgcgtgcg cttgaactgg aagagattcc gggtatcgtc
     8821 aatgatttcc gtcaggccat tgctaacgcg cgtgaagccg cttttgatct ggtagagctc
     8881 cactctgctc acggttattt gctgcatcag ttcctttccc cttcttcaaa ccatcgtacc
     8941 gatcagtacg gcggcagcgt ggaaaatcgc gcacgtttgg tactggaagt ggtcgatgcc
     9001 gggattgaag aatggggtgc cgatcgcatt ggcattcgcg tttcgccaat cggtactttc
     9061 cagaacacag ataacggccc gaatgaagaa gccgatgcac tgtatctgat tgaacaactg
     9121 ggtaaacgcg gcattgctta tctgcatatg tcagaaccag attgggcggg gggtgaaccg
     9181 tatactgatg cgttccgcga aaaagtacgc gcccgtttcc acggtccgat tatcggcgca
     9241 ggtgcataca cagtagaaaa agctgaaacg ctgatcggca aagggttaat tgatgcggtg
     9301 gcatttggtc gtgactggat tgcgaacccg gatctggtcg cccgcttgca gctcaaagct
     9361 gagcttaacc cacagcgtgc cgaaagtttc tacggtggcg gcgcggaagg ctataccgat
     9421 tacccgacgt tgtaatccaa cattgcgagc ggcgtaaagc cgccgctata ctaaaacaac
     9481 attttgaatc tgttagccat tttgaggata aaaagatgcg tcttcttcat accatgctgc
     9541 gcgttggcga tttgcaacgc tccatcgatt tttataccaa agtgctgggc atgaaactgc
     9601 tgcgtaccag cgaaaacccg gaatacaaat actcactggc gtttgttggc tacggcccgg
     9661 aaaccgaaga agcggtgatt gaactgacct acaactgggg cgtggataaa tacgaactcg
     9721 gcactgctta tggtcacatc gcgcttagcg tagataacgc cgctgaagcg tgcgaaaaaa
     9781 tccgtcaaaa cgggggtaac gtgacccgtg aagcgggtcc ggtaaaaggc ggtactacgg
     9841 ttatcgcgtt tgtggaagat ccggacggtt acaaaattga gttaatcgaa gagaaagacg
     9901 ccggtcgcgg tctaggcaac taatctcctg ccgggcgtga actcatcgcg cccgcatctt
     9961 tactgcatcg acaagtaata tttgtcataa tgcgcgctgc aatttatccg tattaagaga
    10021 atcagatgtc cgataacgct caacttaccg gtctgtgcga ccgttttcgt ggtttttatc
    10081 ctgttgtgat cgatgttgaa acagccggat ttaacgccaa aaccgatgcg ctgcttgaga
    10141 ttgccgccat caccctgaaa atggatgaac aaggctggct gatgccggac accacattac
    10201 atttccacgt cgaaccattt gtcggcgcaa atttgcaacc agaagccctc gccttcaacg
    10261 gcattgaccc gaacgatccc gatcgcggcg cggtcagcga atacgaggcg ctgcacgaaa
    10321 tttttaaagt tgtacgtaaa ggtattaaag cgagcggctg taaccgcgcc attatggtgg
    10381 cgcacaacgc caattttgat cacagcttta tgatggccgc cgcagaacgc gcctcgctga
    10441 aacgtaaccc gttccaccct ttcgccactt ttgacactgc tgcactggcc gggctggcac
    10501 tcggacaaac cgtattgtca aaggcttgcc agaccgctgg catggacttc gacagcaccc
    10561 aggcgcactc cgcgctgtac gacaccgaac gcactgctgt gctgttttgt gaaatcgtca
    10621 accgctggaa acgtctggga ggctggccgc tacctgccgc cgaagaggtg taatcgagtc
    10681 gatgcctgat gacatgcaat gattcaggca tctatagtga ggctattcca cgcatcaagc
    10741 atgatattca cggggaatag cgttaatggc agataatcca gacccttcat cgctcctgcc
    10801 ggacgtgttt tcaccggcga cccgcgactg gtttcttcgc gcctttaaac agccgaccgc
    10861 tgtccagtcg caaacctggc atgtggcggc gcgaggcgaa catgcgctgg tgattgcacc
    10921 gactggctcc gggaaaacgc tggcagcatt tctctacgcc ctcgatcggc tcttccgcga
    10981 aggcggcgaa gatacccgcg aggcgcataa gcgtaaaacc tcacgcatcc tctatatttc
    11041 accgataaaa gccctgggca ccgacgttca gcgcaacttg cagatcccgt tgaagggtat
    11101 tgccgatgaa cggcggcggc gcggcgaaac ggtaattatt gatgaagtcc acgcggtagc
    11161 gggcagtaaa cgtggtgcgc atctggcgtt aagtctggag cggctcgatg cgctgctcca
    11221 cacctcagca cagcgaattg gcctttctgc cactgtgcgc tcagccagcg atgtggcagc
    11281 atttcttggt ggcgatcgcc cggttacggt agtcaacccg cccacaatgc gccatccgca
    11341 gatacgaatt gtcgtaccgg tcgccaatat ggatgatgtc tcatcggtcg ccagcggcac
    11401 cggcgaagac agccatgccg gccgggaagg ctccatctgg ccatatattg aaacgggtat
    11461 ccttgatgaa gtgttgcgcc atcgctcgac cattgtcttt actaattcac gcgggctggc
    11521 ggaaaaactg acggcacgat taaatgagct ttacgccgca cgcttacagc gttccccgtc
    11581 tatcgccgtt gatgcggccc atttcgagtc gacctccggc gcaacctcta accgtgtaca
    11641 aagtagcgac gtttttattg cccgctcaca ccacggctcc gtctctaaag aacaacgagc
    11701 aatcaccgaa caggcgctga aatcgggtga attacgctgc gtggtcgcaa cctccagtct
    11761 tgaactgggg attgatatgg gcgcggtgga tctggtgatt caggtggcaa cgccgctttc
    11821 tgttgccagt gggttacaac gcattggtcg cgccggacat caggttggcg gtgtatctaa
    11881 agggctgttt ttcccccgta cccggcgtga tttagtcgat tccgcagtca ttgtagagtg
    11941 tatgttcgca ggcaggctgg aaaacctgac accaccgcat aatcctctcg acgtccttgc
    12001 gcagcaaacc gttgccgccg cggcgatgga tgcattacag gtagacgaat ggtactcccg
    12061 cgtacgccgt accgcaccgt ggaaagatct gccaagacgt gtttttgacg ccacgctgga
    12121 tatgctttcc gggcgctatc cctctggcga tttttctgct tttcgcccca aactggtctg
    12181 gaacagggag accgggatat tgaccgcccg acctggcgct caattgttgg cggttaccag
    12241 cggcggcacc attccggatc gtggcatgta tagcgtgtta ttacccgaag gtgaagaaaa
    12301 ggccggttcg cggcgggtgg gtgaactgga tgaggagatg gtatatgagt cgcgggtgaa
    12361 cgacattatc actctcggcg ctacctcatg gcggatccag caaatcaccc gcgatcaggt
    12421 gattgtgact cctgctccgg gtcgttctgc ccagctcccc ttctggcgtg gtgaaggtaa
    12481 cggacgtccg gctgaattag gcgagatgat cggcgatttt cttcatttac tggcggatgg
    12541 cgcgttcttt tccgggacta ttcccccgtg gctggcagaa gaaaatacga acgccaatat
    12601 tcaggggttg attgacgagc aacgcaacgc gacgggcatc gttccgggga gtcgccatct
    12661 ggtcctcgaa cggtgccgtg atgaaattgg cgactggcgt attattttgc actctcccta
    12721 tggaagacgg gtgcatgaac cctgggcgct ggcgattgcc gggcgaatac atgccctatg
    12781 gggcgctgac gcgtctgtgg tcgccagtga tgacagcatt gttgcgcgca ttcctgacac
    12841 tgatggtaaa ttgcccgacg ccgcgatttt tttgttttaa ccagaaaagt tgctgcaaat
    12901 tgtccgcgag gcggtaggca gctcggcact tttcgccgcc cgttttcgcg aatgcgccgc
    12961 gcgggcatta ttaatgccgg gccgcacgcc gggccatcgc accccgcttt ggcaacaacg
    13021 gctgcgcgcc agtcagttgc tggaaatcgc tcagggatat ccggattttc cggtcattct
    13081 cgaaacccta cgcgaatgtc tgcaagatgt ttatgatctt cccgcactgg aacgtttgat
    13141 gcgtcgcctg aacggtggcg aaattcaaat atccgatgta acgaccacta cgccctcgcc
    13201 tttcgccaca agtttattgt tcggctatgt cgcggaattt atgtaccaga gcgacgcccc
    13261 gctggcagag cgccgggcat ccgtactgtc gctggacagc gagttactgc gcaatctact
    13321 cggacaggtc gatccggggg aattactcga cccgcaggtc attcgccagg tggaagaaga
    13381 gttgcaacga ctggctcctg gtagaagagc gaaaggtgaa gaaggattgt tcgacctgct
    13441 gcgcgaactg gggccaatga ccgttgaaga cctggcgcaa cggcatacag gcagcagtga
    13501 agaggttgcg tcgtatctgg aaaatcttct tgcagtaaaa cgaatcttcc cagcgatgat
    13561 tagcggacag gagcgtcttg cctgtatgga tgatgccgcc aggctgcgtg atgccctcgg
    13621 cgtacggcta ccagagtcat tgccagagat ttatttacat agagtcagtt acccgcttcg
    13681 cgacctcttt ctgcgctatc tccgggctca tgctctggtc acggctgaac aactggctca
    13741 tgagtttagt ctcggtattg ccattgtcga agagcagctt cagcaactgc gtgaacaggg
    13801 tctggtgatg aatctgcaac aagacatctg ggtgagcgat gaagtatttc gtcgtctgcg
    13861 tttgcgctcg ctgcaagccg ccagagaagc gacgcgtccc gttgcagcca cgacctatgc
    13921 gcgattgctg ctggaacgtc agggcgtatt acccgccacc gatggtagcc cggcgctctt
    13981 tgcctcaaca tctccaggcg tttatgaggg cgtagatgga gtgatgcggg tgatcgaaca
    14041 gcttgccgga gtcggtttac ccgcctcact ctgggaaagc cagatcctgc ctgcccgcgt
    14101 acgcgactat tcgccagaaa tgctcgatga attactggca accggagcgg ttatctggtc
    14161 gggacaaaaa aaagctgggt gaagatgacg gcctggtggc actgcatcta cagaaatatg
    14221 ccgcagaatc gttcactccc gccgaagcgg atcaggcgaa tcgttcggcg ctgcagcaag
    14281 cgataatcgc tgttctggct gacggaggag cctggtttgc acaacaaatc agccaacgga
    14341 tacgcgataa aatcgtcgaa tcggttgatt cctcggcact gcaagaggcg ttatgggcgc
    14401 tggtctggca aggcgtcatt accagcgata tttgggcacc gttacgcgcc ctcacccgca
    14461 gcagttccaa cgcacgcacc tcaactcgcc gcagtcaccg ggctcgtcgt gggcgtcctg
    14521 tctatgcaca acccgtctcg ccgcgggtat cttacaacac accaaatctg gctggacgct
    14581 ggtcgttatt gcaggtggag ccactaaacg ataccgaaag gatgctggcg ctggcggaaa
    14641 atatgctcga ccgctacggc atcatcagtc gtcaggcggt gatagccgaa aatatccctg
    14701 gcgggtttcc atcgatgcaa acactttgtc gaagtatgga agactccggg cgaattatgc
    14761 gaggtcgttt tgtagaaggt ctgggtggcg cgcaattcgc tgaacgtctg actattgacc
    14821 gattgcgcga tctggcgaca caagccacgc aaacacgcca ctatacacca gtggcgctct
    14881 ccgccaacga tccggctaat gtgtggggaa atcttctgcc ctgggctgca catccggcaa
    14941 cgctggtacc aacgcgtcgg gcgggtgcgc tggtggtcgt ttctggcggc aaattgttac
    15001 tctatctggc gcaaggcggc aaaaaaatgc tggtctggca ggaaaaggag gaattactcg
    15061 ccccagagat tttccaggcg ctgaccaccg cactgcgtcg cgaaccaagg ctgcgcttta
    15121 cgctaacaga agtgaatgat cttccggtcc ggcaaacgcc gatgtttacg ctgctgcgtg
    15181 aggcgggatt ttcaagttcg ccacaagggc tggattgggg atagttgaca tcctccacgc
    15241 cctgaaagac gtggttccct gctacgttca agctgtcgcc tgaatcattt cggtgggttc
    15301 ctgcttcaac gagcggcctg actgcaccat ctctccacag gcaagaacgg cgtgccccgc
    15361 cgctaaaatg ttacgagcgc catttacatc ggcgttcgct gtatatccac atacctggca
    15421 tctgaattta ctttgtgaca ggcggttttc tttcgctgta tgaccacagc acgcgcaacg
    15481 ctggcttgtg tacgctggcg gaacagcaag cacctgaccg ccactccaga gctgcttgta
    15541 ctcaagctgg cggcgcattt cataccagcc ctgatccagt atcgaacggt ttaaacctga
    15601 ttttgcccgg acattgcgcc ccggctggct gaccgtaccc gctgctgact ttgacatgtt
    15661 gctgaccttc aaatcctcaa tgactatcat tgcgtggttt ttgctgacgg tcgttgtgac
    15721 tttgtgaagg tagtccctgc ggatatttgc gatacaggaa tgcagtcgct gtattttgcg
    15781 tttctgcttc tgccagttgt tgctgaattt gaccttccgg cttaactgtc gctgaagtct
    15841 cgccagcgtc ttctggtttt tttgaaaact gtttacaggc tcaaagactg tgccatctga
    15901 cagcgtggcg agtttagcca cgccagcatc cagcccgatc attgatgctg aagggtgaac
    15961 cggagttgat acttcacttt ctgtctgaat actgatgtac cacgtaccgc aggactggct
    16021 gacagtgaca tttttcacaa ctcccgtgac ctgacggcta ttccggtagc gcatccagcc
    16081 aagtttcggc agaaaaatac ggctgttttc ctgatcgagc ttaacgtcct gcgggtagcg
    16141 gaatgcagca ttctgtcccc gctttttgaa tcggggaaaa gcagcccgct tctggaagaa
    16201 gtttttgtaa gcccgctcaa ggtctttcag tgactgttgc aatggctgtg agggcgaatc
    16261 tttaagccat tgcgtttcag tggcgttttt ccactcaacc agccaggaag ccattttccc
    16321 gtaagggatg tatttattac cggcctcatg attctcattc tgaagcgcca gagcacgatt
    16381 gaaaacgaaa cgacatgcgc ctgcgaagcg cctcatttca cgctcctgtt gaccaccggg
    16441 tcttaactgg aatttaaatg cctgtaatcg tttcacaggt caaatgatac tttggtctgt
    16501 gaaaaaagaa accgatatcc ggcgtggaag gcattgtgtt ttcctgatgc atgttcacct
    16561 ggtctttgtc accaaatacc gacgccagat ttttgaccat gacacgacag aaaaactacg
    16621 cacttacttt tcaaatgtat gtgctgattt tgcagctgaa ctggttgaaa tggatggcga
    16681 accagatcac gtccatttgt taattaacta tcctcccaaa ctggcgatat ccagtctggt
    16741 aaacagcctc aaaggcatat cgaataggtt actgcgacga gatcgaccag atattgcagt
    16801 caggtattac tacaaaggcg ttttgtggag tcctggctat tttgccagta gctgcgaagg
    16861 tgcgccaata tccgtcatcc gccagtacat tgaacaacag caaacacctg gtcaggtgga
    16921 aaaccgcgcc ttatatcccc gccctgaagg acgaggtttt acggcgcacc ggataaagga
    16981 ctgacggatg cccgttcgca tccgtcagta ttgcaggacg gattattccg cgtccggctc
    17041 ttcagacttg tatttagcgg cagtttcttt gatcagctgc tgcagttcgc cacgctgata
    17101 catttcgatc acgatatcac aaccgccgac cagctcgccg tcaacccaca gttgcgggaa
    17161 ggtcggccag ttagcatatt tcggcagttc cgcacgaatg tccggattct gcagaatatc
    17221 aacataggca aaacgttcgc cacatgcggc aagcgcctgg actgcctggg cagagaaacc
    17281 gcagctcggc agtttcggtg aacctttcat gtacagcagg atcgggtttt cagcaatctg
    17341 gcgttggatt ttttcgatag tggtgctcat tgtcttgctt cctcaaactt ctttacggca
    17401 gtaatctgac attgtagcgg gtcagtgcgg catcggaaaa taacattttc atcacgcttt
    17461 tgctatttta tccctttgct ctatttgttg catttcaaat attcgtttct tctctgatgc
    17521 gcggcaaact ggcgtattta taaccatttt tagctttcac tgctgcattt ttttgcgctc
    17581 gccaacgaaa cgtatttttt aacaataaaa gctattaact ttctcttctt ctatgcatta
    17641 gaatcatcaa gttttgtaaa tcagacgcag gcatgataga cctgccttaa cagagggacg
    17701 ctcagtggcg cggataaacc gtatttcgat cacgctctgt gctttacttt ttaccaccct
    17761 gcctttaacg cctatggccc atgcttcaaa gcaagccagg gagagttctg ctaccactca
    17821 tatcaccaaa aaagcagata aaaagaaaag cacggcaacc accaaaaaga cccagaaagc
    17881 cagccagaaa acagcgaaaa aagccgccag taaaagtatg accaaaagca aaaccgcttc
    17941 ttctgttaaa aaatcttcca ttaccgcttc taaaaacgcc aaaactcgca gcaaacacgc
    18001 cgtcaataaa acggcctcag ccagctttac cgaaaagtgt accaaacgta agggctataa
    18061 atcgcattgt gtgaaagtca aaaatgccgc gtcaggaacc cttgccgacg cgcacaaagc
    18121 gaaggtgcaa aaagccacga aagtggcaat gaataaactg atgcagcaaa ttggtaagcc
    18181 atatcgttgg ggcggcagct caccgcgtac cggttttgat tgcagcggcc tggtttatta
    18241 cgcttataaa gatttggtga aaattcgtat tccgcgtacg gcgaatgaaa tgtatcacct
    18301 gcgtgatgca ggacctatcg aacgtagtga gctgaaaaac ggcgacctgg tctttttccg
    18361 tactcaggga cgcggtacag ccgatcatgt cggcgtgtat gtcggcaacg gcaaatttat
    18421 tcagtcaccg cgcacaggtc aggaaattca aatcacttct ctcagtgaag actactggca
    18481 gcgccactat gttggcgctc gtcgggtaat gaccccaaaa acacttcgct aaaactttac
    18541 cctgttgtta cggcaacagg gtaagttcat cttttgtctc accttttaat ttgctaccct
    18601 atccatacgc acaataaggc tattgtacgt atgcaaatta ataataaagg agagtagcaa
    18661 tgtcattcga attacctgca ctaccatatg ctaaagatgc tctggcaccg cacatttctg
    18721 cggaaactat cgagtatcac tacggcaagc atcatcagac ttatgtcact aacctgaaca
    18781 acctgattaa aggtaccgcg tttgaaggta aatcactgga agagattatt cgcagctctg
    18841 aaggtggcgt attcaacaac gcagctcagg tctggaacca tactttctac tggaactgcc
    18901 tggcaccgaa cgccggtggc gaaccgactg gaaaagtcgc tgaagctatc gccgcatctt
    18961 ttggcagctt tgccgatttc aaagcgcagt ttactgatgc agcgatcaaa aactttggtt
    19021 ctggctggac ctggctggtg aaaaacagcg atggcaaact ggctatcgtt tcaacctcta
    19081 acgcgggtac tccgctgacc accgatgcga ctccgctgct gaccgttgat gtctgggaac
    19141 acgcttatta catcgactat cgcaatgcac gtcctggcta tctggagcac ttctgggcgc
    19201 tggtgaactg ggaattcgta gcgaaaaatc tcgctgcata ataactgatg gcaaatgcag
    19261 cattgcctga tgcgcttcgc ttaccatgcc aacgcggatg aggcgttaac agcgcattcg
    19321 gcaagataaa tcgcacgttg tcagcaactg taacgcagaa ggttatcctt ctgcgttttt
    19381 gtttaattag ctgttagcaa cgcaaactgt ttcaggttgt tttctggctg acataaacac
    19441 cagcaataat gccagtcccg cgacaatcgc ccccgtcacc ggcacaaagc tgtatcccag
    19501 cccagcggaa attaccgcac caccagcagc tgctcccagc gcatttccaa gattaaaggc
    19561 accaatattg actgatgaag acagacctgg cgcttcactg gcgacatgca tcacgcgcat
    19621 ctgtaacgga ggtacaaccg caaaggttgc tgcgccccac accaccatgc taatagctgc
    19681 gccgaactca ttgcgggcca ggaacgggat tgccagcata atcaccatca gcagcaacaa
    19741 aaagcctttc aacgtgccgt taactgaacg atctgccagt ttgccgccga gatagttacc
    19801 gatagagaat ccgacaccaa tcagcaccag cattgccgtg acgaacaccg gtgttgcgtg
    19861 ggtaatactt tgcagtaccg gagagatata ggtgtagagg gtaaacattg caccagctcc
    19921 cagtaccgtc gtcagcaatg cagacagcac ctgcggacgc attaataccg ccagctcttt
    19981 tttcacttca ggtcgtgccc ctgtaccacc tttaggtaat gagaagaaca gacttaccat
    20041 tgaaatcact cccagccccg ccgttgccag aaatgacatc cgccagccga tggtttcacc
    20101 caaccaggtc gccgccggca cgccaccgat atttgccagg gttaacccca taaacatagt
    20161 ggcaactgcg ctggcctgtt tatgttttgg caccacgctt gcggccacga ctgaacccaa
    20221 accaaaaaat gctccgtgat tcaggctggt caaaatacgt gaaagcatca gggtcatata
    20281 atccggcgcg atggcggaaa gaacgttgcc tagcgtgaaa attgccatca ggaaaatcaa
    20341 cgcactgcgg cgggcacgat gagaaagtag aagcgtcatc agcggcgcgc ccaccattac
    20401 gccaactgca taggcactga ttaacattcc ggcagcggga atcgagacat ccacaccgcg
    20461 cgcaatgacg ggcaacaagc ccattggcga gaactccgtt gtcccgatac caaacgcgcc
    20521 aatcgccagc gccagcaacg gatagttaat tttcatgcct tatctccacc tcttcgcgtc
    20581 attacgcgat attcattaaa gtggcgaaag catgacagca atcacaaaaa aatgaaaata
    20641 acaaaaagag aaaacacttt tgccattttg ctaacaaaca ggaaggagat gcgagggaga
    20701 acgcgctccc tcgagaggaa atcagtgcag cgcggcagtc aaacccacgg ctacgatcaa
    20761 accgaggacg ataaccgttg ttaccagtga aaatttaagg tcggtgctca tcaagttttc
    20821 tcctttttta ttaccacaca aaaagtgata ttacgcattt ttacacactg tgatgaaaaa
    20881 atctcccgtc atttataatg ataagtgttt ttaccacttc cccttttcgt caagatcggc
    20941 caaaattcca cgcttacact atttgcgtac tggccattga ccccttcctg acgctccgtg
    21001 tcgtttttcc ggcgtaccgc aacacttttg ttgtgcgtaa ggtgtgtaaa ggcaaacgtt
    21061 taccttgcga ttttgcagga gctgaagtta gggtctggag tgaaatggaa tggcaacaat
    21121 aaaagatgta gcgaaacgag caaacgtttc cactacaact gtgtcacacg tgatcaacaa
    21181 aacacgtttc gtcgctgaag aaacgcgcaa cgccgtgtgg gcagcgatta aagaattaca
    21241 ctactcccct agcgcggtgg cgcgtagcct gaaggttaac cacaccaagt ctatcggttt
    21301 gctggcgacc agcagcgaag cggcctattt tgccgagatc attgaagcag ttgaaaaaaa
    21361 ttgcttccag aaaggttaca ccctgattct gggtaatgcg tggaacaatc ttgagaaaca
    21421 gcgggcttat ctgtcgatga tggcgcaaaa acgcgtcgat ggtctgctgg tgatgtgttc
    21481 tgagtaccca gagccgttgc tggcgatgct ggaagagtat cgccatatcc caatggtggt
    21541 gatggactgg ggtgaagcaa aagctgactt caccgatgcg gtcattgata acgcgttcga
    21601 aggcggctac atggccgggc gttatctgat tgaacgcggt caccgcgaaa tcggcgtcat
    21661 ccccggcccg ctggaacgta acaccggcgc aggccgcctt gccggtttta tgaaggcgat
    21721 ggaagaagcg atgatcaagg tgccggaaag ctggattgtg cagggtgact ttgaacctga
    21781 atccggttat cgcgccatgc agcaaatcct gtcgcagccg catcgcccta ctgccgtctt
    21841 ctgtggtggc gatatcatgg caatgggcgc actttgtgct gctgatgaaa tgggcctgcg
    21901 cgtcccgcag gatgtttcgc tgatcggtta tgataacgtg cgcaacgcgc gctattttac
    21961 gccggcgctg accacgatcc atcagccaaa agattcgctg ggtgaaacag cgttcaacat
    22021 gctgttggat cgtatcgtca acaaacgtga agaaccgcag tccattgaag tgcatccgcg
    22081 tttgattgaa cgccgctccg tggctgacgg cccgttccgc gactatcgtc gttaatcacc
    22141 cgttgcggga gtctcttccg gctcccgcag ccactcctta ttcagcgtct cactatcgct
    22201 gagatactca agcagccagg ttaacgcagg cgacatatca ttttgctgcc atgtcagaca
    22261 acatgccgaa tccgggaagg ggttttccag ttcaagtgct acccacttcc cctcattaag
    22321 ccacggtttg gcgaaatgtg ttggcaccat ccctatgcat aatcctgccg agatacaggt
    22381 tgccgatgat tcccagtcag gtacgacaag tcttttttgg ttatccagca accaggtaat
    22441 acgtttaggt agcgttcgcg aggtgtcttc gcgcaccaac gacggccagt tgcgcaacgt
    22501 atcatcgctg aacgggccat ccatcaacgc cagcgggtgg tgactggcaa caacgcaact
    22561 ccagcttagc atccccatat cccggaaggc ataacgaccg cctaccggaa tcgcgcgtgt
    22621 tgcgccaatt gccagttcca cgcgcccgtc ggaaagcgca tcccagacac cgttgaacac
    22681 ttcctgaaag acaagaagtt cgacatcatc aaaatggcga taaaaatcaa cgatcatctg
    22741 ccgtgtacgt tctggcctga caatattatc cactgcgata gctaactgac cgcgccagcc
    22801 gttcgctatc tgctgacatt gctggcgggt gatctgcatt tttttgacaa cagagcgccc
    22861 ttctttgaga aaccacgctc cagcagcggt cagctccaca tcacggtgcc gtcgttcaaa
    22921 gagcggtacc gccagccact cttccagctg acgcacggta tagctgaccg cagaaggaac
    22981 acgatgcagc tcctgtgccg cagcgctaaa actaccatta cgcgctaccg catcaacaac
    23041 ttcgagtgaa tattctgacc acatagtctg cctgcaaaat ttttgaaacc agtcatcaaa
    23101 tattaccgtt tcacaacact aatttcactc cctacacttt gcggcggtgt ttaattgaga
    23161 gatttagaga atatacatgc aacctgggaa aagattttta gtctggctgg cgggtttgag
    23221 cgtactcggt tttctggcaa ccgatatgta tctgcctgct ttcgccgcca tacaggccga
    23281 cctgcaaacg cctgcgtctg ctgtcagtgc cagccttagt ctgttccttg ccggttttgc
    23341 cgcagcccag cttctgtggg ggccactctc cgaccgttat ggtcgtaaac cggtgttatt
    23401 aatcggcctg acaatttttg cgttaggtag tctggggatg ctgtgggtag aaaacgccgc
    23461 cacgctgctg gtattgcgtt ttgtacaggc tgtgggggtc tgcgccgcgg cggttatctg
    23521 gcaagcatta gtgacagatt attatccttc acagaaagtc aaccgtattt ttgcgaccat
    23581 catgccgctg gtgggtctgt ctccggcact ggctcccctg ttaggaagct ggctgctggt
    23641 ccatttttcc tggcaggcga ttttcgccac cctgtttgcc attaccgtgg tgctgattct
    23701 gcctattttc tggctcaaac ccacgacgaa ggcccgtaac aatagtcagg atggtctgac
    23761 ctttaccgac ctgctacgtt ctaaaaccta tcgcggcaac gtgctgatat acgcagcctg
    23821 ttcagccagt ttttttgcat ggctgaccgg ttcaccgttc atccttagtg aaatgggcta
    23881 cagcccggca gttattggtt taagttatgt cccgcaaact atcgcgtttc tgattggtgg
    23941 ttatggctgt cgcgccgcac tgcagaaatg gcaaggcaag cagttattac cgtggttgct
    24001 ggtgctgttt gctgtcagcg tcattgcgac ctgggctgcg ggcttcatta gccatgtgtc
    24061 gctggtcgaa atcctgatcc cattctgtgt gatggcgatt gccaatggcg cgatctaccc
    24121 tattgttgtc gcccaggcgc tgcgtccctt cccacatgca accggtcgcg ccgcagcgtt
    24181 gcagaacact ctgcaactgg gtctgtgctt cctcgcaagt ctggtagttt cctggcttat
    24241 tagtatcagc acgccattgc tcaccaccac cagcgtgatg ttatcaacag tagtgctggt
    24301 cgcgctgggt tacatgatgc aacgttgtga agaagttgac tgcccgaatc atggcaatgc
    24361 cgaagtcgct catagcgaat cacactgatc tatatcgata tacttatact taggctgcta
    24421 acaaaatttt gttgtatgat tgaaattagc ggcctatact aatttcgagt tgttaaagct
    24481 acgataaata ttatgttttt acggggacag gatcgttccc gactcactat ggatggtcat
    24541 ttcggcaagg gttcctcctt tccctctgtt ctacgtcgga ttatagactc gcggtttttt
    24601 ctgcgagatt tctcacaaag cccaaaaagc gtctacgctg ttttaaggtt ctgatcaccg
    24661 accagtgatg gagaaactat gagttcatcg tgtatagaag aagtcagtgt accggatgac
    24721 aactggtacc gtatcgccaa cgaattactt agccgtgccg gtatagccat taacggttct
    24781 gccccggcgg atattcgtgt gaaaaacccc gattttttta aacgcgttct gcaggaaggc
    24841 tctttggggt taggcgaaag ttatatggat ggctggtggg aatgtgaccg actggatatg
    24901 ttttttagca aagtcttacg cgcaggtctc gagaaccaac tcccccatca tttcaaagac
    24961 acgctgcgta ttgccggcgc tcgtctcttc aatctgcaga gtaaaaaacg tgcctggata
    25021 gtcggcaagg agcattacga tttgggtaat gacttgttca gccgcatgct tgatcccttc
    25081 atgcaatatt cctgcgctta ctggaaagat gccgataatc tggaatctgc ccagcaggcg
    25141 aagctcaaaa tgatttgtga aaaattgcag ttaaaaccag ggatgcgcgt actggatatt
    25201 ggctgcggct ggggcggact agcgcactac atggcatcta actatgacgt aagcgtggtc
    25261 ggcgtcacca tttctgccga acagcaaaaa atggctcagg aacgctgtaa aggcctggat
    25321 gtcaccattt tgctgcaaga ttatcgtgac ctgaacgacc agtttgatcg tattgtttct
    25381 gtggggatgt tcgagcacgt cggaccgaaa aattacgata cctattttgc ggtggtggat
    25441 cgtaatttga aaccggaagg tatattcctg ctccatacta tcggttcgaa aaaaaccgat
    25501 ctgaatgttg atccctggat taataaatat atttttccga acggttgcct gccctctgta
    25561 cgccagattg ctcaatccag cgaaccccac tttgtgatgg aagactggca taacttcggt
    25621 gctgattacg atactacgtt gatggcgtgg tatgaacgat tcctcgccgc atggccagaa
    25681 attgcggata actacagtga acgctttaaa cggatgttta cctattatct gaatgcctgc
    25741 gcaggtgctt tccgcgcccg tgatattcag ctctggcagg tggtgttctc acgcggtgtt
    25801 gaaaacggcc ttcgagtcgc tcgctaaagg ctattctatc gccccctctt cgggggcgag
    25861 ttcagatcag gcttctgtac ctggttgatt catggcattt tctcgtgccg ccagcacacg
    25921 ttctaccgta tctaccactg cctgagtttg tggatcgatt tcaatgttga cgcgtgcgcc
    25981 aagttttttc ttcccaagag tcgtgcgttc cagtgtttcc ggaattaaat ggacgcaaaa
    26041 acgcgttggc gtgacttcgc cgacggtcag gctaataccg tcgatgccaa taaatccttt
    26101 gtacaggata tatttcatca actgactatc ctggacttta aaccagatct ggcgattatt
    26161 ttccgaggtt aatattttcg ccacttcagc ggtggtcata atatgacctg acattaagtg
    26221 tccgccaatt tcatcactga atttcgccgc acgctcaacg tttacccaat cccccacttt
    26281 taaatcgcca agattggtaa tgcgtaacgt ttctttcatc aggtcaaaac tgacatggtt
    26341 gccgttaatt tccgtcacgg tcaggcagca accgttatgc gccacggaag caccggtttc
    26401 caggccgtcc agcatgtggt cgggtaactc caccacatgc gtacgaaaat ttggtttctc
    26461 gtcaatcgac accagttttg cggtgccctg tacaatcccc gtaaacatac ttacaactcc
    26521 tgaaatcagt taagacattc tgttcagcac aatagcaggt ggaaaacgcc cttaccagtg
    26581 aaggggtaag aatggctatt ttttcactgg agaattaata aatcctcgct acaatagact
    26641 gaatttcccc tgcttcttct ttttgctgcc cattcaggcg gctttttagt ctctcatata
    26701 actacaaata aaaggtgttc acgtgcagaa gtatatcagt gaagcgcgtc tgttattagc
    26761 attagcaatc ccggtgattc tcgcgcaaat cgcccaaact gcgatgggtt ttgtcgatac
    26821 cgtgatggcg ggcggctata gtgccaccga catggcggcg gtcgctatcg gtacttctat
    26881 ctggcttccg gcgatcctct ttggtcacgg actgctgctg gcattaacgc cggttatcgc
    26941 gcaattaaat ggttccggtc gacgtgagcg cattgcgcat caggtgcgac aaggtttctg
    27001 gctggcaggt tttgtttccg ttctcattat gctggtgctg tggaatgcag gttacattat
    27061 ccgctacatg gaaaacatcg atccggctct ggcggacaaa gccgtgggtt atctgcgtgc
    27121 gttgttgtgg ggcgcgccgg gatatctgtt cttccaggtt gcccgtaacc aatgtgaagg
    27181 tctggcaaaa accaagccgg gtatggtaat gggctttatc ggcctgctgg tgaacatccc
    27241 ggtgaactat atctttattt atggtcattt cggtatgcct gagctcggtg gcgttggttg
    27301 tggcgtggct actgcggcgg tgtattgggt catgttcctt gccatggttt cttacattaa
    27361 acgcgcccgc tccatgcgcg atattcgtaa cgaaaaaggc accgctaagc ccgagcctgc
    27421 ggttatgaaa cgactgattc aactcggttt gccgattgcg ctggcactgt tctttgaagt
    27481 gacactgttt gccgtcgtgg ctctgttagt gtctccgctc ggtattgttg atgtcgcagg
    27541 acaccagatt gccctgaact ttagttcact gatgtttgtg cttccaatgt cgctggcggc
    27601 agcggtaact atccgcgtag gttatcgtct gggtcagggc tcaacgctgg atgcgcaaac
    27661 cgctgcgcgg accgggctta tggtgggtgt ctgtatggca accctgacgg ccattttcac
    27721 ggtttcactg cgggagcaaa tcgccctgtt gtacaacgac aatcccgagg ttgtaacgct
    27781 ggctgcgcat ttgatgttgc tggcggcggt atatcagatt tctgactcaa tccaggtgat
    27841 tggcagtggg attttgcgtg gttataaaga tacgcgttcc attttctata tcacctttac
    27901 agcttactgg gtgctgggct tgccaagcgg ctatattctg gcactgaccg atctggtcgt
    27961 tgaacctatg gggccagcag gcttctggat aggctttatt atcggcctga catcggcagc
    28021 cattatgatg atgctgcgta tgcggttcct gcaacgtatg ccgtcagcca tcattctgca
    28081 acgagcgtcc cgctaataaa gacaaggcgc aaccttcacg ggttgcgcct gtgtttttac
    28141 gcaggctgga gcgttgcgcc aatcccgtct tcgtctggct gtaatttcag agcgttacac
    28201 agagtttgcc gactggcatc tgttatctca acgcgtatat ccccgttaac ttcccctttc
    28261 acattgatga agtcaggtga aatttctttc gcactggttt gcaggcaaat cgctccctct
    28321 ccgcgcaggc gtttcacttt cagttgcaca tgcgttgccg caccagtgat cagccacagt
    28381 tttccgccat tcagattgat gttaccaaca atgaaagtga cgctgtcacc ttttaccgta
    28441 ctgccgtcga gaatcaacac accgccctgc tgtacagtaa tcgttgattg ttgcagctct
    28501 gcgccataac caataatcat tgaagcgccg tccagtaatg caacactccc ggtcagttca
    28561 accggtgcgc gatcttcctc tacgacaaaa ttttcgtccg cgctggtggg tgacaacagg
    28621 atcactgttc caccttgtat taccgcattt tccagcgtac cggcatgcac ttcagcccgc
    28681 ccccccacat tcaccgacag gttttgcgtc ttaccggaac tgtagagctg aaggccatcc
    28741 gtccccagac gataaatgga tccattctca acaatagtgt cagagtcttc accaccgaga
    28801 ttattcatgg tgccaccagc aagcaacgta tcactggctt tcccggccag cataaaaacg
    28861 ccgccctgat taatctcaca attgctggct tctccacttg gatacaccaa caattcacca
    28921 ccttcatcta ccacgaccgc tgaggttgtc ccattaacca acaggccgcc ctcttgatcg
    28981 agaatgattt tttccgcgcg atgtccttcc agtacacgca ggttaccgcc attttccagc
    29041 aacaaaccgc aggcataacc ctgatcaacg ctgaattcac cttcgggatg gcggccatta
    29101 accgtagcga gcgtagagag actaattgcg cctccctcgg actgaacaat atctgtggca
    29161 atggcaccgt cgctgaccga tagcgtgcca ttgttaccgt actgtgtgca tttggcgatc
    29221 ccaccggttg ccactttcaa ccagccagcc cccgtgacca cggtattttc ggctcttccg
    29281 ccagcttcaa caatcaactc gccttcagta agttgcgtcg cgccaaggac ctcgccggtc
    29341 ttaacaattt gcacattccc gtcgctcatc aagtttttcg catcagatcc catgatttat
    29401 tcctttgctg catctgtgtg cctttattgc tacctaagtg taaaggctac ggaggattta
    29461 tccacgacag atttgagatg gtggcaaaca actctgttta aactctgata cacgaattat
    29521 tgggttgtat cagatgtaaa tgcgatcctg aataaaaatc gcccttgcaa atcaacaaaa
    29581 tatagccaat ttgcttaaaa gctctgcaaa cggtcgaaat agcgcagaaa attacgtttt
    29641 gcctcttgcc acctttccac tctgccgcta atattcgtcc ccgttgtcac ctacaacgtt
    29701 gcgttcatag ctcagttggt tagagcacca ccttgacatg gtgggggtcg ttggttcgag
    29761 tccaattgaa cgcaccatcc tgcgtccgta gctcagttgg ttagagcacc accttgacat
    29821 ggtgggggtc ggtggttcga gtccactcgg acgcaccaga ttttcttaat ctggtctttt
    29881 cctttttccc tctgtttctt ctctgtatcc aatacgttaa aagatttaca ctgtcttcgt
    29941 atgcgttatc agaaggagaa tcgctatggc aactttgtta caacttcatt ttgcttttaa
    30001 tggcccattt ggcgacgcaa tggctgagca gcttaaacca cttgctgagt cgattaatca
    30061 ggaacctggt tttctgtgga aggtatggac agaaagtgaa aagaaccacg aagccggtgg
    30121 gatctacctt ttcactgatg aaaaaagcgc ccttgcctat ctggaaaaac atactgcccg
    30181 actgaaaaat ctcggcgttg aggaagttgt cgccaaagtt ttcgatgtca atgagccact
    30241 tagtcaaatc aatcaggcaa aactcgcctg acagaattta atcaagggcg gttagcgccc
    30301 ttttcatccc tgtctgaaat ttctcaaatt ctaaaaatct caaccaaact tatctgataa
    30361 cactaaattc gaaagaatgc gtacaggtaa gtaacaatga aaaaaattgc tattgtgggt
    30421 gccgggccta cggggatcta caccttattc tcgcttctac agcaacaaac tccactttct
    30481 atttctatct tcgagcaggc tgacgaggcc ggtgtcggga tgccatacag tgatgaggaa
    30541 aactcaaaaa tgatgctggc aaatattgcc agtattgaaa taccgccgat taattgtacg
    30601 tatctcgaat ggctacaaaa gcaagaagcc agccatctcc agcgttatgg cgttaaaaaa
    30661 gaaaccttgc acgatcgtca gtttttaccg cgaattctgc tgggcgaata tttccgcgat
    30721 caatttttac gattagtaga ccaggcacga cagcaaaaat ttgcagtggc tgtttatgaa
    30781 tcatgccagg ttaccgatct gcaaattaca aatgctggcg tcatgctcgc tacaaatcag
    30841 gatttaccca gcgagacgtt tgatttagcg gtgattgcca cgggtcacgt ctggcctgat
    30901 gaagaagaag caaccagaac gtattttccc agcccgtggt caggcctgat ggaagcaaag
    30961 gtcgatgcgt gtaacgtggg tattatggga acatccttga gcggactgga tgcggcaatg
    31021 gcagtggcta ttcagcatgg ttcgttcatt gaagatgata aacaacacgt cgtttttcac
    31081 cgcgataacg caagtgaaaa gctaaatatt acgttgatgt cgcgcacggg tattttaccc
    31141 gaagccgatt tctattgccc tattccctac gagcccttac acatcgtcac cgatcaggca
    31201 ttaaatgctg agattcaaaa aggtgaagag ggccttttgg atcgggtatt tagattgata
    31261 gtagaggaaa tcaagtttgc tgatccagac tggagccaac gcatagcctt agagagcctg
    31321 aatgtcgatt cctttgctca agcctggttt gccgagcgca aacaacgcga cccatttgac
    31381 tgggcagaaa aaaatctcca ggaagtcgaa cgcaataaac gagaaaaaca tactgttccc
    31441 tggcgttatg tcattctgcg cctgcatgaa gccgtacagg aaattgttcc tcatctgaat
    31501 gaacacgacc ataaacggtt cagtaaaggc cttgccaggg ttttcatcga taattatgcg
    31561 gcaatccctt cagagtctat tcgtcgacta ctggccttac gtgaagcggg gatctgaaat
    31621 ccctgatgtt ggcgaagatt atacgttaca gcaacccgaa gatattcgtg gacgcgtagc
    31681 gttcggcgcg ttgccctggt tgatgcacga ccagcctttc gttcagggac ttacggcatg
    31741 tgcagaaatt ggtgaggcga tggctcgggc ggtcgtaaag cctgcatccc gtgctcgtcg
    31801 gcgtctttcg tttgattaaa ggtaaagcta tcagcactca acaaactgat gctgttcatt
    31861 aagacgatag cggactccgg ttcgaatatt gttttcacct tcaatggcga cggcgaagcg
    31921 aactctttcg ccgtcatgat atgccagtgc ggcgcttccc cctagcccga gaataattga
    31981 gtcgaccacg cccgtgctca ctaccgttga attttcaccg ctggcaatga tgtgtacatt
    32041 gtcgccgctg ttggcaattc tggcgtttgc gccaaagctg gcgatttgca ccaggtcacc
    32101 attactggcg acatgacacc gttcacccaa tgtacaaacg cgtactcgca ttccggtatt
    32161 cgcaatccga ctactatctc cagcgctgct tatccgcgaa gaattaccgg ttacagcaac
    32221 ccgtgcgcgt tcaccggagc tgccaatgtg actgttaaag ccgacacttc caatccgcac
    32281 gctataaccc atactggcaa tttgcgcggc atacccagcg gttgcgattc gggcgttatc
    32341 ctcacaacag ccgatgcgag catgatcgtc gctgcgggca aactgactgc aaaatggtat
    32401 ctctccgggg tctgtggcca gtttttgcat agaggaaact tcctgatgcg cgaagttttc
    32461 atccgccagc cattttttcc acgcatactc aaccagactc tccgcccagt ccgtatatcc
    32521 ttcctcaatc agtgcatgac gaatatcggc ataactaccg ccgcgtggga aatggcgcag
    32581 gaaccagcga tacatcaccg ctccgatacg ccattctcgc aggtctgctc gtgtaatgat
    32641 cattcgttag tgccggtgat agccatcgac catgctttta aaggtttcgt gtggcgtacg
    32701 ccccgtggtg caaagataaa gatgaccgaa gataaaaaag aggcttataa atgccagagc
    32761 aaaatgcgcc tgcaataacc agtatcttac gccaggaaac acatctccca cggcttgcgg
    32821 atagagacac aacagccccg tcaatagtaa caacggcagc aatccataca tgacaccaac
    32881 ataggcgacc tgctgtaagg gattaaattt agactgtgtt gttgccggga aaggatgttc
    32941 ttccccctgc ataatgccaa acaaataaaa tcgcgtttgt tttgccgctc gttccagcca
    33001 cccctgacga cgaatgcgat agtggtgacc attatcccca acggcattga tcagcacaaa
    33061 gccgagccag catgccagta acaaaaatcc gcaaacttca tgcaccgcaa ccagactttt
    33121 aaccgcagtt gcgcccacca tcgcaaagtg atttatcagc ccacttgcca gcaacaatac
    33181 aaagagtaac gcattcgacc agtgccataa tctgaccgct ttgctgtaaa gataaacctt
    33241 ctcaccatga tcggtggctg atttcttcac gccacgagca cgaagcaagg cgtgcagccc
    33301 cagcaccaac cacatcccaa ccagcaatac tccggcaatg atcaaccaca ccggccaaaa
    33361 ttcgggagtg aactgtggca catagttcgc taactggctc tgaaactgtt cagcatgttg
    33421 cgacgggttc atacattttc ctttttaatc aaatgttgac cgaaccggcg atacagatgc
    33481 ggttttccgg caccaggtag ctgatattga tagtatttat tatcctgcaa ccacgcctga
    33541 atttctgggc tatcttcgcg accaaagatc aatgcgtgtt ccggacaggc gctgacgcaa
    33601 atgggcggaa agccttttgc cagtcgggac tcagcgcaga aatcacattt atctgccact
    33661 ttagtgacag ggttaaggta gcgaaccagg tacggacaag cgccaatgca ataactgcag
    33721 ccgatgcact gcgatttttc tacccgcacg atcccctgct catcacgcca cgatgccccc
    33781 gtcgggcata cgtcgatgca cggagcatct tcgcagtgct gacatgactg gcgaaaaaag
    33841 tgatactgcg tttcgttgtc gttatcggtg actggaatgt gtgctatcga taaacggctt
    33901 ccctgagcgg ggacgtggtt agttttacgg catgcgcggg cgcaaatatt acagccattg
    33961 caccgtgact cgtcatgaat catggcatac cgaacttcct tttcttgcct cgtgttcgct
    34021 aacagagaag acgcagaacc agtaaaaaag attactgttc ccatccccag aacaaatttg
    34081 cgtcgagtga aggacatctg attatcccct aatgcagtgg ctgcaactgt ccaccctatc
    34141 catgacatct ccctgaaatt tcaggggttc tgaaactgct ctacctcgaa ccattcgcat
    34201 aagccataag ccttgccata ggtcataaat cgctcgctaa acaagtacaa caccgccgtc
    34261 gatgactcca cctgccgtat atcggcatat tcaggtgcaa catccatcgc ggcgatcgcg
    34321 gcttcaatct gcgcttcctg gaagtaatat ggcgcttgca tcagcatggc gactttgtaa
    34381 ggacgaggat acgtctggca ctcaaaacga acggcgtgtg caatggcgcg acaaatgtcc
    34441 tgctccacaa cttgcaaaga catcgccgcg tagttttcac tcatggcctg cgttgagtaa
    34501 tagtaatcat cctgacttcc cgtcatacaa gcgatatccg cgtagtctcc gttctgctta
    34561 atgctttcca gcaaaactga cagttcctct tctgcaaccg aatagggtgg cgagagaaac
    34621 acggtgcgcg ccaccagctg cccagccgca gaatgctggc gaatgaactc agccaggatt
    34681 tcagcaggtt ctggttcggt taccgtatca acggggtgac gcatcccctg caaccattgc
    34741 cgcttcgcct cgtcaacttc gctgactttc gctaacggca attcatcctg atggttcatc
    34801 ttacccattc cttacgcagg cagtagattg tgtgctgcca gatcggcggc aatatcttcc
    34861 agccccagac gctgcaatgt ttcgcgggtt ggacaaccaa gctgtgggtc ccagcccatt
    34921 tctttgtaga acatcgtgag cgaagcatgc atatcgtcac gatccatttt gtcagtacct
    34981 tcagtaaaga ccgggatctg cggatccttg tcgaaaaccc aggaacagat aagatcgtgt
    35041 tcattacgca tatctttggt ttgcatcggt tttaccgtgt aggcacgatg caacgtaaag
    35101 atacgctctg cagctaaatc taatttttcc tgagtcatct cttcgccggt gatcgctttg
    35161 aagaatttgg cttcaagcgc cagatcgccc cggtaattac ggcttttcag cggcgaaacg
    35221 gtcattggcc agacccagtt gcacagagtg acggcgttat gcaaacagac ccgcaacagc
    35281 gaccactttg catatttaat ttttgcgtca ttgattggcg tgtagttttt ggtttcatcg
    35341 taagcatctt cagaaccaaa aagttctttc gccacttcac gttgcagttt caatggcaag
    35401 ccggaaccaa taaagttgat atgggtatgc gtcatgcagt cacggttgaa catacactta
    35461 acaatggaac cgacctgcgc tgacgcttca ttggcatggt gaaccggata gccaaacggc
    35521 gaccagagtt tatttttcgc gtagccccag tactctttac ccaaattcca gcgttctgcg
    35581 atggcatatg aaccatcagc caggtgactc agctcaccca cacgatgcgc cagacggtag
    35641 taaaaatctt taatgaagtt aacgtcaccc gcttccagtt gatcccagcg aatttctgca
    35701 tactcttcag ctggcagaac acgcttgaac acacctttgc tgtaacaata agtaaagtcg
    35761 cgatgcaact gcccgtagtt acaccatagg ccgtagtcat cgaacaaatt cagaccaacc
    35821 aggttaccaa tcacacgacc atcgtcttta tcttcaaaat ctttcggccc gttcgggaag
    35881 atggtggtat ggacaaagtt tgctacacaa gtgttaccac ctgtgctggg cacgccaaac
    35941 tctttcaccc gaggaatatt catttgggtc atacaacgga tcgggcaaga gtggcagccg
    36001 ctcattttta ctgtgtattt ctctgccgcc ggtcctaagt caaaaacgga tttataggta
    36061 cgaaagccga ccgtattctg attgcctggc ggaatttcac ccgtttcaat cggaccacct
    36121 tcagccgcgc cccaaaacag ccctttacgt gctgtccagc gtgacttggg atctgaatac
    36181 tctgcccacg attgtggcgt acttggcacg acatggttgt tattcgcacc aataagttca
    36241 gtcatcatgt aatcattgag acgcttcatc tcctgacgat cggcaatgtt gacccctttc
    36301 gtcccttcaa ccgcaatcgc tttcaggttt ttcgaaccca ttattgcgcc agttcccgca
    36361 ccgccgctgt ggttacggct attcaacatg ccagagagag gaataaggtt ttccccagcc
    36421 tgaccaatag ccgccacaca ggtttccggg ctggtcaatc gacaaatttc ttccgtcgtt
    36481 gcgcgcgtcc cttttcccca taagaaatcg gctttttcca ggctaacttt gtcatcttta
    36541 atcttcagcc ataccggtga tttcgccttc ccttcgataa taatgacgtc gtatccagcg
    36601 aatttcattt gcgctgcaaa aaagccaccc atatgggcat cgcgactaaa tttcctttgg
    36661 taaaagtaga aagtgaggtg atatttacgc gagaactaca gggggcacca gatccaggta
    36721 atgggccggt agcaaagact aatttattcg cttcatcgaa aggtttcgtg cctagcggta
    36781 cttcgtcata cataattttg tagccgaagc ccatgccacc gacaaaactt ttaaacttac
    36841 tggaatcttc gagggtaata tttcctgtcg tgagattgac tcttaatata ttacctgtcc
    36901 aaccgttagc catgattttt tcctttgcaa gattcacaca gtaatatctt tccactcgat
    36961 aatttttaac gcccctgttg ggcaggcgtt tgcgcattca ccgcataaca cacatttcga
    37021 ggatttttta ctttcggtat ttacggtggc catcatccac gggcatgccg tagtacaggc
    37081 gctacagcca atacaacgtt tatgatcgac ggtaatacag ccttctttct gctgccaggt
    37141 aatcgcacca atcgggcaga cgttcatgca ttgcggctct ttgcattgac ggcaggtgtc
    37201 cgcggtatag ttgagatcgc catacaggcc gccgccagag ccaaccccgt tgtcgccaaa
    37261 gaaataattg cgatggattt tgatacggga gaagaatgtc cctactgagc catcgttgaa
    37321 gttggtacaa gagatttcac agcggtggca acccgtgcat cgcgctcgct gggtcaccag
    37381 cacccctttg ggtgtattta tcaaccctac tgtgccgcta tcaatatctt cctgtttgca
    37441 acccaaaagt gacagtaacg caggggcaat tgttagacca gcaaggcctt ttcctgatat
    37501 acgcaagaat tctaatcgcg ttaagccaat atctaatagt ggacgatcaa ccgggttcat
    37561 ttatttcgca tcctcttcgc aaacacagtg ctgacaattc agcagcctga tattaagcat
    37621 tcaactaata cgctgctgac tctgtcccca acaccagata ttttccctaa cccgatagtg
    37681 tatgagaaaa gatttaacgc cgataaacta atcctttatg gttaggagaa aaaataatgt
    37741 gattattctg agcccttaac attgatcgtt atcaattaaa attacaaagc aagaaattat
    37801 aagcgaactg atatttatta tcatttgaaa taaatttaac ttaagcgaca atcattt
//
LOCUS       DAEBXN010000030        36587 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.030, whole genome shotgun sequence.
ACCESSION   DAEBXN010000030 DAEBXN010000000
VERSION     DAEBXN010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36587
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.030"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(156..1391)
                     /locus_tag="KJL99_001567"
     CDS             complement(156..1391)
                     /locus_tag="KJL99_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HBD4436336.1"
                     /translation="MLQQVPTRAFHVIAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENNFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAVRDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLMVVNK"
     gene            complement(1538..1614)
                     /locus_tag="KJL99_001568"
     tRNA            complement(1538..1614)
                     /locus_tag="KJL99_001568"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1578..1580),aa:Pro,seq:tgg)
     gene            complement(1657..1743)
                     /locus_tag="KJL99_001569"
     tRNA            complement(1657..1743)
                     /locus_tag="KJL99_001569"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1707..1709),aa:Leu,seq:cag)
     gene            complement(1764..1839)
                     /locus_tag="KJL99_001570"
     tRNA            complement(1764..1839)
                     /locus_tag="KJL99_001570"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1804..1806),aa:His,seq:gtg)
     gene            complement(1898..1974)
                     /locus_tag="KJL99_001571"
     tRNA            complement(1898..1974)
                     /locus_tag="KJL99_001571"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1938..1940),aa:Arg,seq:ccg)
     gene            complement(2077..3462)
                     /locus_tag="KJL99_001572"
     CDS             complement(2077..3462)
                     /locus_tag="KJL99_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBD4436337.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWSPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3653..4393)
                     /gene="rffM"
                     /locus_tag="KJL99_001573"
     CDS             complement(3653..4393)
                     /gene="rffM"
                     /locus_tag="KJL99_001573"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HBD4436338.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISIVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4396..5748)
                     /gene="wzyE"
                     /locus_tag="KJL99_001574"
     CDS             complement(4396..5748)
                     /gene="wzyE"
                     /locus_tag="KJL99_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HBD4436339.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQVLLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(5745..6824)
                     /gene="rffT"
                     /locus_tag="KJL99_001575"
                     /gene_synonym="wecF"
     CDS             complement(5745..6824)
                     /gene="rffT"
                     /locus_tag="KJL99_001575"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HBD4436340.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASADKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(6821..8071)
                     /gene="wzxE"
                     /locus_tag="KJL99_001576"
     CDS             complement(6821..8071)
                     /gene="wzxE"
                     /locus_tag="KJL99_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HBD4436341.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8073..9203)
                     /gene="rffA"
                     /locus_tag="KJL99_001577"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(8073..9203)
                     /gene="rffA"
                     /locus_tag="KJL99_001577"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HBD4436342.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDTLAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9208..9882)
                     /gene="rffC"
                     /locus_tag="KJL99_001578"
                     /gene_synonym="wecD"
     CDS             complement(9208..9882)
                     /gene="rffC"
                     /locus_tag="KJL99_001578"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HBD4436343.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTSDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(9860..10741)
                     /gene="rfbA"
                     /locus_tag="KJL99_001579"
     CDS             complement(9860..10741)
                     /gene="rfbA"
                     /locus_tag="KJL99_001579"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HBD4436344.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(10760..11827)
                     /gene="rffG"
                     /locus_tag="KJL99_001580"
     CDS             complement(10760..11827)
                     /gene="rffG"
                     /locus_tag="KJL99_001580"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HBD4436345.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELASN
                     KPHGVAYYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(11827..13089)
                     /gene="wecC"
                     /locus_tag="KJL99_001581"
     CDS             complement(11827..13089)
                     /gene="wecC"
                     /locus_tag="KJL99_001581"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HBD4436346.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(13086..14216)
                     /gene="wecB"
                     /locus_tag="KJL99_001582"
     CDS             complement(13086..14216)
                     /gene="wecB"
                     /locus_tag="KJL99_001582"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HBD4436347.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGALYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIAPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14272..15318)
                     /gene="wzzE"
                     /locus_tag="KJL99_001583"
     CDS             complement(14272..15318)
                     /gene="wzzE"
                     /locus_tag="KJL99_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HBD4436348.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15330..16433)
                     /gene="wecA"
                     /locus_tag="KJL99_001584"
     CDS             complement(15330..16433)
                     /gene="wecA"
                     /locus_tag="KJL99_001584"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HBD4436349.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(16673..17932)
                     /gene="rho"
                     /locus_tag="KJL99_001585"
     CDS             complement(16673..17932)
                     /gene="rho"
                     /locus_tag="KJL99_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HBD4436350.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(18017..18118)
                     /gene="rhoL"
                     /locus_tag="KJL99_001586"
     CDS             complement(18017..18118)
                     /gene="rhoL"
                     /locus_tag="KJL99_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HBD4436351.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18259..18588)
                     /gene="trxA"
                     /locus_tag="KJL99_001587"
     CDS             complement(18259..18588)
                     /gene="trxA"
                     /locus_tag="KJL99_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HBD4436352.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            18719..19984
                     /gene="rhlB"
                     /locus_tag="KJL99_001588"
     CDS             18719..19984
                     /gene="rhlB"
                     /locus_tag="KJL99_001588"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HBD4436353.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            20118..21602
                     /gene="gppA"
                     /locus_tag="KJL99_001589"
     CDS             20118..21602
                     /gene="gppA"
                     /locus_tag="KJL99_001589"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HBD4436354.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENDLSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQENQWQSYVHWPLEVH"
     gene            complement(21649..23670)
                     /gene="rep"
                     /locus_tag="KJL99_001590"
     CDS             complement(21649..23670)
                     /gene="rep"
                     /locus_tag="KJL99_001590"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HBD4436355.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEAHGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNYQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSTKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            23757..24038
                     /gene="ppiC"
                     /locus_tag="KJL99_001591"
     CDS             23757..24038
                     /gene="ppiC"
                     /locus_tag="KJL99_001591"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HBD4436356.1"
                     /translation="MAKTAAALHILVKEEKLALDLLVQIKNDADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(24084..25559)
                     /gene="ilvC"
                     /locus_tag="KJL99_001592"
     CDS             complement(24084..25559)
                     /gene="ilvC"
                     /locus_tag="KJL99_001592"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HBD4436357.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIVRKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            25709..26599
                     /gene="ilvY"
                     /locus_tag="KJL99_001593"
     CDS             25709..26599
                     /gene="ilvY"
                     /locus_tag="KJL99_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HBD4436358.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPLLYGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMANQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(26596..28140)
                     /gene="ilvA"
                     /locus_tag="KJL99_001594"
     CDS             complement(26596..28140)
                     /gene="ilvA"
                     /locus_tag="KJL99_001594"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HBD4436359.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(28143..29993)
                     /gene="ilvD"
                     /locus_tag="KJL99_001595"
     CDS             complement(28143..29993)
                     /gene="ilvD"
                     /locus_tag="KJL99_001595"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HBD4436360.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALCAYASLATSADKGAVRDKSKLGG"
     gene            complement(30058..30987)
                     /gene="ilvE"
                     /locus_tag="KJL99_001596"
     CDS             complement(30058..30987)
                     /gene="ilvE"
                     /locus_tag="KJL99_001596"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HBD4436361.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQIGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(31007..31270)
                     /gene="ilvM"
                     /locus_tag="KJL99_001597"
     CDS             complement(31007..31270)
                     /gene="ilvM"
                     /locus_tag="KJL99_001597"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HBD4436362.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(31267..32913)
                     /locus_tag="KJL99_001598"
     CDS             complement(31267..32913)
                     /locus_tag="KJL99_001598"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HBD4436363.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(32916..32966)
                     /locus_tag="KJL99_001599"
     CDS             complement(32916..32966)
                     /locus_tag="KJL99_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4436364.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(33052..33150)
                     /gene="ilvL"
                     /locus_tag="KJL99_001600"
     CDS             complement(33052..33150)
                     /gene="ilvL"
                     /locus_tag="KJL99_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HBD4436365.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            33502..34981
                     /locus_tag="KJL99_001601"
                     /pseudo
     CDS             33502..34981
                     /locus_tag="KJL99_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
     gene            complement(35006..35344)
                     /gene="maoP"
                     /locus_tag="KJL99_001602"
     CDS             complement(35006..35344)
                     /gene="maoP"
                     /locus_tag="KJL99_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HBD4436366.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            35463..36302
                     /gene="hdfR"
                     /locus_tag="KJL99_001603"
     CDS             35463..36302
                     /gene="hdfR"
                     /locus_tag="KJL99_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HBD4436367.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(36398..36473)
                     /locus_tag="KJL99_001604"
     tRNA            complement(36398..36473)
                     /locus_tag="KJL99_001604"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36438..36440),aa:Trp,seq:cca)
     gene            complement(36482..36558)
                     /locus_tag="KJL99_001605"
     tRNA            complement(36482..36558)
                     /locus_tag="KJL99_001605"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36522..36524),aa:Asp,seq:gtc)
BASE COUNT         8942 a        10012 c         9215 g         8418 t
ORIGIN      
        1 gccaccgcac gtaaaggctt tgccggatac gatcacccaa accagtatcc acagaacgct
       61 gcaatttatt gaatttgcac gatcatgtag gccggataag gcgtttacgc cgcatccggc
      121 aatcaaccgc aggcggcttc caaatggccg cttttttact tattcaccac catcaaatgt
      181 gcctgcataa tgtcactggc cgggcgaccg tgcgccagca aatcagccat tgctttaaga
      241 tatggcggta gatggcggaa ataacgctga tacccggcac acaaataatt cagccccggt
      301 ttgccgctgg catcgagcat gaagcggtgt ttcgggcagc ctccccaaca tgcttttaac
      361 acgttacaac tgcgacactg cgccggtaat tgcttaaatt tatcttcacc aaacacctgc
      421 tgttgcgggg aatcgatcat ttctgcaatt gtttgctgat gcatattccc cagccgatat
      481 tgcggataaa catagtgatc gcaggcgtaa acgtcgccgt tgtgctcaac aatcaccgag
      541 cgcccacagg ttggctgatg atggcaaacc gcacccggcg caccgacaaa attggcaaac
      601 gcccattcga tattcatcac gaaaatcttg ccgacgtcgc gtttgatcca gtggtcaaaa
      661 atcgccacca gaaattcacc aaattcatcg gggcgcacag accattccgt cagttccccc
      721 tgaatatcac caggcgcatg tagtttcagt ccatcgcgga cagctgtttc atcagccagg
      781 cgctcgacca ccggaataaa ctggatgaat tcgactcccg catcgcacaa aaaatcataa
      841 acctgtaacg gttgctgcgc gctggtgcgg ttgacgcaga ccagcacgtt atagtcgaca
      901 tgatgttttt gcaggagcgt cagggcacgc atcaccagtt tatgcgtggg tctgccgcct
      961 ttggtcacgc gatattgatt gtggatctca gccggaccat ccagcgataa cccaacaaga
     1021 aaattatttt ctgccagaaa tgcacaccat tcgtcatcga gcagcacgcc gttagtctgg
     1081 aaactgttac ttatcttcct gccagcacca tatttcgcct gtagcgccac ggcacggcgg
     1141 taaaactcca gcccgagtag cgttggttcg ccgccctgcc aggtaaaagc cacttcgttt
     1201 tgcagttcgc tggcggcgat atagtgacgg atatacgctt ccagcgtgtc atcgtccata
     1261 tgcgtgactg gcttttcgcg gtaaagggat tgtttttcga gataaaaaca gtagtcacag
     1321 ttcagattac aatcggaacc gctcggtttc gctatcacat gaaaagcacg cgttggaacc
     1381 tgttgcagca tggtcgctcc ttgcctgaag tcatccttca atacgttaag ggcgaaatca
     1441 taccgtgggg caatgagtta ttacgggcgt ttattgcgaa gggagggtga cgaaatgcac
     1501 agaaaacaaa aaaccccgcc gaagcggggt tcaaaattgg tcggcgagag aggattcgaa
     1561 cctccgaccc actggtccca aaccagttgc gctaccaagc tgcgctactc gccgaaatac
     1621 tgctttttga atttttagtt caattcttta aagtcgtggt gcgagggggg ggacttgaac
     1681 ccccacgtcc gtaaggacac taacacctga agctagcgcg tctaccaatt ccgccacctt
     1741 cgcattgtca caacttctaa taatggggtg gctaatggga ttcgaaccca cgacaactgg
     1801 aatcacaatc cagggctcta ccaactgagc tatagccacc actacaaatc ttgttacgcg
     1861 gtattactac caccgcagct caagcgctgg gactaaatgg cgcgcccgac aggattcgaa
     1921 cctgagacct ctgcctccgg agggcagcgc tctatccagc tgagctacgg gcgcttagcg
     1981 ccgttgcggg ggtggataat acggacttcc cacccctctg tctagtccct ttttaaataa
     2041 attgctcgtt tggttacggt ttgtgcgttt tgctgcttat tcctccagtt tatgcgcttt
     2101 accgtggcga ttaagcccaa aaactttata aatcgccgtc accgccagca taaagatgat
     2161 gccgacaaac agcgacatac gcgtatcttc attaaagtac atgccgatca aaacgcaaat
     2221 caggaatgcc attgttacgt agttggccca cgggaacagg attgagcgga acggatggct
     2281 ggcaatcgcc gctttatgtg cacgccgaaa acgcagctgg cttatcaata tcacaaacca
     2341 tggcaccatc cccggaagca cgctggcact gtagacgtag acaaacacac gctgcggatt
     2401 gggaatgatg tagttcaggc atgagccaat tagcagaata gcaatagata ctgccacacc
     2461 cgcaaccggt acgccgtgac gggaaacttt tgccattgcc gccggtaact gacggttttt
     2521 cgccagtgcg tagagcatac gtccgcaact gtacatgccg ctgttacagc cagagagcgc
     2581 agccgtcagc accacaaagt tgataatgcc cgccgctgcg gtaataccga ttttggcaaa
     2641 agtcagtacg aacgggctgc cgttgctgcc aatttcattc cacgggaaga tggtgacgat
     2701 aacgaaaatc gcgcctacgt agaaaatcag gatccgccac agcactttgc ctacggcact
     2761 gcgcagcgtc acctgcggat tcttcgcttc accggcagta atgccaatca gctccacgcc
     2821 ctggtaggac gccaccacaa tacacagagc ggtcaggaac cctttccagc cacccgcaaa
     2881 gaaaccgcca tgctctgtga gattgctaaa accaatcgac tgcccgccat tgccaaagcc
     2941 aaagaaaatc acgcccaggc caatgacaat catcacgata atcgtggtga ctttgatcat
     3001 cgcgaaccag aactcgattt cgccgtacaa ccgtaccgcc gccagattcg ccaacgccac
     3061 cagcgccacc gcgatcaatg cgggtatcca ctgcgccatc tccggggacc aaaactggac
     3121 ataaacgcca atggcggtga tttctgagat ccccaccgcc atccacataa accagtaaga
     3181 ccaggcggtg agatagccaa aaaacgggct catataacga tgcgcataaa cggcgaacga
     3241 accggtaacc ggttcgagga acaacatttc gcccattgaa cgcatgatga aaaaaacgaa
     3301 cagcccggcg atgatatagg ccaacaatac ggatggcccg gcccatttca gggtactggc
     3361 ggcccccata aacaggccga cgccaatggt gccccccagg gcgatgagtt cgatatgtcg
     3421 agcttccagc ccacgctgta gctctggttt gttatctgcc ataaatcctc gtgttgtgtt
     3481 tgcatgcttt ccggtgttac cggttatcgt tatgggtaca tcgagtgttg cgaatgtttt
     3541 cgtaattcag gagaaatggc aaataaagca ttaaaaattt gaatgctttg tgtaataaaa
     3601 aagcagacag gcgacgaagt gaccactccg tcgctttaca aagagaggaa aatcagaggt
     3661 tgccggtgta gtgccagcgt aaataacgca gcaaacgaag ctgacgctta atgcggctcg
     3721 gctgcgaaag caggcggtag agccactcca gtcccagcgt ttgccagatt ttcggtgcgc
     3781 gttttacgtg accggtgaaa acatcgtaag tcccgccaac gcccatatac agcgcatctg
     3841 gatgcaccag acggcagtcg cgcatgaaga tctcctgctt tggcgatccc atcgcaacgg
     3901 tgacgatttg cgctccgctg gcatgaatgc gttcaaacag cgcctgacgc tgctcgggtt
     3961 taaaataacc atcctgactg ccaacgatat tcacattcca ttggttgcgc agtttagctt
     4021 cagtttgcgc cagcacttca ggtttaccgc ccacaagaaa taccggcgtc ccttctttgc
     4081 ctgcgcgcgc catcagctct tcccagagat cggcaccggc aacgcgggaa acctgcgcct
     4141 gcgggtactt tttacgtact gaacgtacaa tgctgatgcc atccgcatat ttaaattcgg
     4201 cagcgttaat taactccctg acctcggcgt tatcttcaat ggtcagcatt ttttcggcat
     4261 taatggcaac cagcgttccc tgcttaagct gcccgtcagc aaacagataa tcgagggcat
     4321 gctgcatatc acgccaacca atcaactgta agccacgcag cgtataggtc ggtgccgtgg
     4381 tgttgttatt cattgttatc cttcaacctg cgtccggagc gatgattttg tacgtttatg
     4441 aatgagtccg gcgctttcaa aaagccagta caacagtttt gcgatcatca gacatgcgcc
     4501 gaagaccacg ataaaaaaga ctacgcgtga gacaaacgaa tccagccctt cacgtgccag
     4561 cacgatcata ttgaagatcg ccccaaagca gaaactgtgc aatatcgcag ccttatagcg
     4621 attagtctcg cggttgccca gctcatacag ccagtcgaac catttgatga tcagtccaac
     4681 cacaatcgcc ccgagtggga tgaacaacgc gccgcccatc accaccagtg agcctataag
     4741 cgtaggcgag atcgccagtc cggagtggtt attcagtact tcccaggtaa agtaattggc
     4801 tgagttcagc accatactcg ggcgccccgg ccacagccag gaagggataa agacatagaa
     4861 atcgcggaca attggagcca ggccctggaa gtcgatgttg tcgtagttct gcaacagcaa
     4921 cgccagattc tcccacggtg aaaaggtgtc gcgagtgaga tagagaaacg tatagaacgc
     4981 ttcatcgccg ctcacattca ttccatagcg ttttaacgcc agccagaaca tgccaacaat
     5041 ccccagcacg cccgccgccg ccagcatcca caacgaaatc cagccgcgaa taatgccaat
     5101 aaacaggaag atagcgaatg cgatgatgat attggcgcga gtgccgccga caatcatata
     5161 agtcagcaag ccaaaggcga ccgtgctgac gaggaaaaac agccacgctt tgctgtcctg
     5221 gcgcagaaag tagaccacca gcatcgccgg gatgaaaaag taaaagaagc gttttaacgc
     5281 cacgccggag acttcactgg aaaagatctg actgtaggag ttaagccgga acagcaaaaa
     5341 gccgttgtgc atgaagaaga tgccgacgct taccagcgcg atacccatca ggatcaccca
     5401 cgtaagattg gtctccacgc ggttcatggt aaacagcgga cggcgcggta catcagcaac
     5461 gcgtttgcgt aggcgggttt tgtaggtgac atagtaaacc gcgtagaagc agcccgcaga
     5521 aagcaacacc tgcaacaaga tttctggcgg cgcgacacca acatcaaagc gaaataccag
     5581 cacgctggtc agcgggaagc cgaagaaaaa ggtgagcaaa aacagcaatg aaaagaagac
     5641 attgaagtta aagcgcacac ggcgaaactc aaaccaggtc agcgtggcaa taaacagcgt
     5701 gcagagcagc cagaccacaa acaggccact gaattgcagc agactcatgc gacctccccg
     5761 gcggcaatcg ccaacgcccg ctgccagcct tgtagatagt tagggctaaa gaaggcaatg
     5821 gtgtttttat ccgccgacgc caactggcgc tgcgcttcac gcacaatatc ctcgttgaga
     5881 tcgtcggtag taaacagcac tggcaaatgc tgttccgtca tatcctgcca gaacggattt
     5941 tccctgttaa gcacacaagg aatgcccgcc tgaatcagta agcaaagcgt accaatgccc
     6001 tgctggcggg caaaaataaa gtaaccaaga tcgcactgac gaagtagcgc cagataggcg
     6061 tcaaattcca gtttttcgct caaaatttgc agattttctt cgctgaataa ctccagcccc
     6121 gcctgacgaa cttcctcaat gtacgcttcg ttattaggcg gatatcccat cggcaccacc
     6181 acttttaccg tatcgccaaa ttgctgatga acggcacgta aagcggcgac atgctcattg
     6241 ctgcggtcgc cggagttccc caccagaatg gtcatttttc cttcacgttg ccgatcgttc
     6301 gccatcgtat tgagagaagg gtccatccgc gtcgggaagt acagcagttc tccccgcacc
     6361 tttgggtgtg ttttggcaaa aaagctcaaa tcgccgcggg tggcaaatac acagccgact
     6421 cgcttttgcg ccaggcgacg tagtgggtaa aaaagcttat atctcaagcc actggaaagc
     6481 tcatacaggt ctgcccccca gatatgccag aaaaactggc tgggcttaat gccaccactc
     6541 agcagagcca gccacagtgt gggattgaac tgaccgtgga aaaaaaatcg ctgctgacgg
     6601 ttagcttttg ctttcgcgat gaccgcttcc gccagcgatt ttttcccagg gaaaaattgc
     6661 acagaaagcg ccggacagct atcacttaag ccgtcgtcct tgccaacaac cataaactcg
     6721 cgcgcgtgct cgctcgtcgc ggccagcgcg tcattgaaaa accgcaaaac ggttcggtta
     6781 tggtgaggga tatccgatcc cagtacgtga atcagtacag tcatgcccgc ctacgccaga
     6841 gtaaaaacac gccacaacaa agagaaaaat agacgatata agttgccata tatgcctgcg
     6901 ccgcgcccag tgcaccatgc gcagggatta gccagtgggc aaataccatc aataaagtga
     6961 actggctgac ttccgccaga atataaaacc gcagtgacgc tttggcgatc accagataac
     7021 caaagacata agcgcccact tttaacacat cacccactaa ctgccaggca aagagatcgc
     7081 gcatagcggt aaatttattc gacaacagca accagatagc aaaatcacgc agcaaccaga
     7141 cggtaaaact cgctgccgcc actgccggta agacgaattt cagcgattta accacttccc
     7201 gggtgatatc gcgcttttcc gttagccgcg acaacgtggg cagcaaatat acgctgaacg
     7261 atgccgtaat aaattgcagg taggcatcgg aaatactgct caccccttgc cagatcccca
     7321 cttcatccca gctatactgc gccgccagca gtttacgcat catgatgtaa gcaacaggca
     7381 aggtcaccga cgtaatcaac gccatgagcg taaatttgct caactgccct gccagaccgt
     7441 tatcccagct gggtttcaga tagcttaacg ggatgacacc acgtttaatc aacatgatgg
     7501 cggcaggaat taccaccagc gcgggaatca gcgccagacc cagcaacgcc ccttcataac
     7561 cgcccaaacg gtaactgacg tagtacgcga gaacgccaat caagctgccg acaatcagcg
     7621 ataacgcatt acctgcggca tcgcgaaagc ctttcatcag cgccagtaac aggttgcccc
     7681 aggcgatccc catttgcacc agcgccacta aacgcaccag cccctgatag tcggtattac
     7741 caaacaatcc ctggctgatt ggcgcagctg ccagcacaaa aaccagcgcc atcagcgtag
     7801 agaaaccaag taccatcgct gatgaagtgc cgaccacgcg gcgcagctgt tgcggattat
     7861 catggtactg ggcaacgtat ttggttacac cgttaaagat gccagccccg gcaagcacgc
     7921 cgagcacggt aatcaactgg cggaaatttg ccgccagccc aagccccgcc ggaccaaatg
     7981 acaccgccag caacttaccg accagtaacc cggcaccaat cttgaccagt gtactggccg
     8041 ccgtccacaa ggacgctttt gccaacgaca tatcaggaga agtagttcaa caaagtcgca
     8101 attaccgtac gctgattgac gggcgacagg ttgtagaaca gcggcaggcg cagcaggcgc
     8161 tcgctctctt tggtggtgta gcgatcttca ccgtggaact caccaaagtg ttcccccgca
     8221 gggcaaccgt gcagcggaat gtaatgaaac accgccatga tttccgcttc tttcagaaag
     8281 ttaatcaacg cgctccggtc atcaatatcc cgcagtttaa tgtagaacat atgcgcgttc
     8341 tgcacgcagc catcgggaat cgacggcagc tcgatacgcc cggctttcgc cagaggcgct
     8401 aacgtatcgt agtagttttg ccacagcgcc agacgttgct ggttgatacg atccgctgct
     8461 tccagttgcg cccacaggta cgcagcttgc agatcggaca tcaaatagct ggagccaata
     8521 tcgcgccagg tatatttatc gacctgacca cggaagaact ggctgcggtt agtgcccttt
     8581 tcacggatga tctcggctcg ttcgattaac gctttatcgt taatcagcgt cgcgccgcct
     8641 tcaccacccg ccgtgtagtt tttggtttca tggaagctaa agcagccaat atgaccaatg
     8701 gttcccagtg cacgcccttt gtaagtggac atcacgccct gagcggcatc ttccaccaca
     8761 aacaaattat gctttttcgc caacgccata atggtgtcca tttcgcaggc cacacccgcg
     8821 taatggaccg gcacgataac gcgcgttttg tcggtgatcg ctgcttcaat caacgtttcg
     8881 tcgatgttca tggtgtccgg gcgaacatcc acaaagacga ttttcgcgcc acgcagcaca
     8941 aaggcattgg cggtggagac aaaggtgtag ctcggcatga tcacttcatc gccaggctgg
     9001 atatcgagca gcagcgccgc catctccagc gaagcggtgc aggacggcgt cagtaacact
     9061 ttggcgctgc caaaacgttg ctccagccac tgctggcagc gacgggtaaa accgccatcg
     9121 ccacacagtt tgccgctacc cattgccgac tgcatatagt cgagttcggt tcccaccacc
     9181 ggcggtgcgt taaatggaat catgtgatca cctgtataac cagtacgcgg tgctttctac
     9241 attcgcacca ctttgtatgt atcgtttaag cgcggcggtg ttgcccattt gggtcgccac
     9301 ccgcaaagtt gttttaccgc gagcatacgc ccagtttagc gccgtttgca tcagctcagc
     9361 acctgcaccg cgtccagcca gcaggccaat tcgcgcatct gtcgcattga gttcccgtaa
     9421 agagacatag ccgcgaatat cgccggacgc cgcacgtaga atcagacatt gatgatcaaa
     9481 ggtgccgcgc acggcatttt caatccactg tgcataaaag cgaccgctgg cgtcaggcgc
     9541 ataccacggc gcacgaaaac ggctttgcgc aaatgcggcg ctagctaact gacgtaatgc
     9601 gggaatatcg gtctcttgtg ccacgacagc accgctatca ctgacattgt tcacgggtag
     9661 cgccaaatca acttcacctt ctaccaggga gaatcccagc tgttgcaggg catccagttc
     9721 ccccgtattt gatgccgcaa ttttggcctg cacccgtgac catggcgcta aggcgtctga
     9781 cgtcaggagc ggtgcttcag acgtaatgcg cacgatggcg ctgttaacac caaagaaggc
     9841 gttttcccag gttagtggct caatactggc gcggacgggc acgaagtaac tccagcagat
     9901 attggccgta gccagttttc gctaatgaac tggcagcacg cttcacaccc tcgtcatcga
     9961 gccagccgtt acgccaggcg atctcttcca ggcaggcaat cttaaagccc tggcgttttt
    10021 ctactgtctg cacaaaagtg ctggcctcaa tcaggctgtc gtgagtgccg gtatccagcc
    10081 aggcaaatcc gcgcccgagc agttcaacgg tcaggttgcc cgcctcgagg tacatctggt
    10141 tgatggaggt aatctccagt tcaccgcgct ccgacggctt cacctgcttt gcgtactcca
    10201 cgactttact gtcgtagaaa taaagcccgg tcaccgccca gtttgacttc ggctgttttg
    10261 gcttttcttc aagcgagata gcgcggaaat tgtcgtcaaa ttccaccacg ccaaagcgtt
    10321 ccgggtccat cacctgatag ccaaaaaccg tcgccccttc cgtgcgcgcc gcaacatgac
    10381 gcagcttcgg actgaagccc tgaccgaaga agatgttatc gcccagcacc agacaagaag
    10441 gttcaccatt aaggaaggtt tcaccgatga taaaggcctg cgccagaccg tccgggctgg
    10501 gctgttcggc atattccagc tggataccga actcactacc atcgcccagc aggcgctgga
    10561 aataaccttt atcttccggc gtagtgatga tgagaatttc gcggataccg gccagcatca
    10621 gcaccgacag cggatagtaa atcattggct tatcgtaaat cggcaacagt tgcttcgata
    10681 cgccgcgcgt aatcggatgc aatcgggtac cggaaccgcc cgccaggata atacccttca
    10741 tgtttgcctc cgctgaaaat tagcctttca gacctaaacg ctcaccctga tagctgccgt
    10801 cctgcacctg cttccaccag ctttcattag ccagatacca ctgaaccgtt ttacgcattc
    10861 cactttcaaa ggtttcctgc ggcagccagc caagttcacg ggcaattttc gaagcatcaa
    10921 tggcataacg cagatcatgc cccggacggt cagcgacaaa ggtgatcagg tcacgatagt
    10981 acgccacgcc gtgcggcttg ttcgaagcca gttcttccag cagctcgcaa atggtttcca
    11041 caacatcgag attcttacgc tcgttgtgac caccaatatt ataggtttca ccgactttcc
    11101 cggtggtcgc cacgcaatac agcgcgcggg cgtgatcttc cacatacagc cagtcacgga
    11161 tttgctgccc gttgccatat accggcagcg gtttacccgc cagcgcgttg aggatcatca
    11221 gcgggatcag tttttccgga aagtggtaag gaccgtagtt attcgagcag ttggtgatca
    11281 gcgtcggcag accgtaggtc cgcagccagg cgcgcaccag atggtcgctg ctggcttttg
    11341 acgcggaata agggctgctc ggcgcatacg gcgtggtttc ggtgaagaaa tcatccgtcg
    11401 agtgcaggtc accatatact tcgtcggtgg agatatgatg aaaacggaac gctgattttt
    11461 tatcttccgt cagcgtattc cagtaagccc gcgccgcttc aagcaatgta taagtcccga
    11521 caatgttggt ttcaataaac gctgccgggc cgtcaataga acggtcaaca tggctttcgg
    11581 ctgccagatg catgacacag tctggctgat gctcagtgaa tacgcgtgcc agttctgccc
    11641 gatcgcagat atcaactttc tcaaaggcaa agcgctcgct ttgcgcgacc ggtgccagcg
    11701 acatcaggtt tccggcgtag gtcagcttat cgaccactac caccgcatcg ctcgtttcgt
    11761 tgatgatata acgcaccagc gccgagccaa taaacccggc accacctgtt atcagaattt
    11821 ttctcatcag cgccagactc ctttggcatc gacgacatac tgctgatgga cattgtcgcc
    11881 attgataact ttgaactgac tatgatcgac cagcatcacc agcacgtctg ccgttgccag
    11941 cgcctcgtca agctgcgcca gagtacaaag cccggtcagt tttttcggca actggtggat
    12001 gttaggctca acaaccagcg tttcgccgct gtgccactgg gcgatcagtt cagcgatttc
    12061 catcgccggg ctttcgcgca ggtcatcaat attcggttta aacgccagac caaagcaggc
    12121 gattttcagt tcactggctc gtttatcggt agcagcaagg cagtcggcaa ccgccgcttt
    12181 cacctgatcg ataacccaga acggtttgtg atcgttaact tcgcgcgcgg tacggataag
    12241 ccgcgcctgc tgggggttct gtgccacgat aaaccacgga tcaacagcaa tgcagtgacc
    12301 gcccacgcca gggccaggct gaagaatatt aacgcgaggg tgacgattcg ccaggcgaat
    12361 cagttcccag acgttaatcc cctgatcggc acaaatcagc gacaattcat tagcaaaagc
    12421 gatattcaca tcgcggaagc tgttttcggt gagcttacac atttccgccg tccgcgagtt
    12481 agtgacgaca cactcacctt cgaggaaaat tttgtacaat tcgctggcgc gggccgaaca
    12541 aaccggcgtc ataccaccaa tcacgcgatc gtttttaatc agctcgacca taacctggcc
    12601 cggtaataca cgttccgggc agtaagcaat gttgacgtcc gcctgctcgc ccacctgctg
    12661 tgggaaagtc agatccgggc gcatctctgc taaccattct gccatcttct cggttgaccc
    12721 caccggcgag gtggattcaa ggatcaccag cgcgcctttt ttcagcactg gcgcaatgga
    12781 gcgagcagcc gattcaacgt aggtcatatc tggctcatga tcgcctttaa acggcgtagg
    12841 tacagcaatc agccaggcat ccgcttcaac tggcgtcgtg ctcgctcgta aaaaaccgcc
    12901 ttctacggca gtttttacta cgctcgccaa atcaggctcg acgatatgga tttcgccacg
    12961 attgatggta tcaaccgcgt gttggttgat atcgacacca atcacctgtt tttgccgtga
    13021 ggcaaacgct gctgctgtag gcagcccgat gtaacccagt ccgataacag aaatggtcgc
    13081 aaaactcata gtgatatccg attatttttt aacgcttcca gaatgcgaga gcatgcctga
    13141 ccatcaccat acgggttatg ggcgcggctc atagcttgat attcgttttc gtcttttaaa
    13201 agacgcgtca cttcctcgac aattcgctgc ttatccgtgc ctaccagacg caccgtaccc
    13261 gccgtcaccg cttccggacg ctcagtggta tcgcgcatca ccagcaccgg tttccccagc
    13321 gaaggcgctt cttcctgaat gccgcctgag tcagtcaaaa tcagccaggc gtggttcatc
    13381 agccagacaa acggtaaata ctcctgggga tcgatcagaa tgacattttt cacatgcccc
    13441 agaatgcgat tgaccggctc tctgacgttc gggttgagat gcaccggata gacaatctgg
    13501 atgtcctggt gcgtggtggc gatgtctgcc agcgcgtggc agatttcttc aaagccacga
    13561 ccgaaactct cacgcctatg accggtcacc agaatcatct ttttatcggg ggcgataaac
    13621 gggtaatttg ccgccagttc tgaacgcagc gtgtcgctgc tcatcacctg gtcacgcacc
    13681 cataacagtg catcaatgac tgtattaccg gtaatgaaga ttcggctatc cgcaacgttt
    13741 tcacgcagca agttttgccg ggaagtttcg gttggagaga agtgatacat cgccagatgc
    13801 ccggtcaatg tacggttagc ctcttccggc cacggcgaat agagagcgcc cgtgcgcaga
    13861 ccagcctcaa cgtgaccaac aggaatacgc tgataaaacg ccgccaggct ggttgccagc
    13921 gtcgtcgtcg tatcgccgtg aaccagcacg acgtctggtt tgaactcggc aagaataggt
    13981 tttagccctt ccagaatccg acaggttatc tctgtcaggc cctgtcctgg ctgcattatg
    14041 ttgagatcgt agtcaggtac aatggaaaag agtttcagta cctgatcaag catctcccga
    14101 tgctgcgcag tgacgcaaac tttagcctca aaaaaaggat cttttgccaa cgcatgcacc
    14161 aacggagcca tcttgatggc ttccgggcgc gtaccaaata cagtcagtac tttcacatcg
    14221 attctcttcg aataagcggc gagcgccttt gcgctcaccg cagcagtgtt gctatttcga
    14281 gcaacggcgg gttaatgcga caccagcccc gatcagcccc ccgacaatgc cccacataat
    14341 catcaggaag gcacgacgtg ggctatcgcg ttttaccggt tcttccggcg tacgcaaata
    14401 gcgataggtc tgaaaacgcg gatccagggt tggaccaaca ttcagggtgt ttaacatggc
    14461 tcgattctga tcatagtcga gatcaaaggc cggaccgacg gcctgtaaat tttccagtcg
    14521 agcctggagc attggacgcc caagcaggaa catttctgaa tcaggtaatt cctcggcagg
    14581 cacatctgtc gcactgcgcg aaatattatg ctgctcagca attttcagcg cctgctcaat
    14641 actgttcatc cggcggtcgt agatggcttt cgccacctct tcctgacgct tcacctgagc
    14701 tttcatctgg atggtgcgtg ccgcccatgc gcctttcagc tcatcattca gatggctggc
    14761 tgcacgctgg ctggcaaaag caacatactg acgtaacagg ttattagcgt caggcgcggt
    14821 ttcggcaata agcttcacgc tgtcattgac cgcgcgagta aagtctccgg ggataaactg
    14881 gatgttgtta atcatttcat ccagcaacgc cgcatcggct ttgctgttgc ccaccatccg
    14941 ctgtttgtaa tagtcggttt gcagccagaa ctctctgcgg gtatcccacg aggccagttg
    15001 cataacaaac tctttatagg cttcgtccat gaccgatggt tgatcggcag aagccatgtt
    15061 tgaacggaca tccaggttac gcaaaaattg ctgctgcgag taatatcccc ccagcatatt
    15121 caccgttgga cgatcggtaa tcgccgtcga gctccactcc tgacgagcaa aaaaagtata
    15181 cgccagcgcg attaacgcaa acgccagccc catgccaata atccatagct tcccagccca
    15241 caaggtacga aacaacccac gaatatccag ttcattttca gcgtcttcgg ccggtttccc
    15301 aggcattggt tgtgtcatca catcctcatt tatttggtta aattggggct gccaccacga
    15361 tttctacgca gtctgcgttt tacgcgctta ataaagcgag caactttcca ggcacgctta
    15421 atgcaatatc catagaggaa gaatgctagc aaaaagagca ccagcatgac ccactccggg
    15481 acaaaatgag aatattctgc cagcacgcca atggaagcga gcagtgctgc ggcaagggta
    15541 atcagcacaa acgcctgacg ggaagtaaac ccggcacgca tgatcaaatg gtgaatatgc
    15601 tgacggtcag gagagaatgg gctcatgcct ttacgcaggc gacggtacat aatcgccacc
    15661 atatccatta gcggaatggc gattatccac aaagcggtaa ccgggctgat gggatgggtt
    15721 ttgccctggg tcgtttcgag caggatccag ataacggtaa aaccaatcag cgtactgccc
    15781 gcatcaccca taaagacttt gtagcggcga cccaggatac caaggttaag catgatgtat
    15841 ggcaggatgg cggcgatcat cgcaaagcac cagattgcga ggctggtttg cccgtcgaac
    15901 cacaaaatca taccgattgc tgcaaacgag acgcaggaca acccgcccag caagccatca
    15961 atgccatcaa ccatgttgaa cgcattaatg gccgcccaga cggcaaatag cgtcaggaag
    16021 taaccaaacg gtccgagcac catctcccag gagccaaaga tataacccag gctactgaga
    16081 taaagtttgc cgaacaccat cataacaatg ccaacagcgg cctgtatggt ggcacggatt
    16141 tttacgctga tatcaaaacg atcatccagc gcgccaatga aaacaagcac accggcacaa
    16201 gcgagataga gagatgcatg cggaatatag taatcgacaa ttccgaacgt gaagcaaatc
    16261 cctgcgtaaa ccgaaatccc cccaacgaga ggtatcaatc cctggtgacg tttgcggaag
    16321 tttggtttat ccactaaacc gacttttttt gccaccttac gggcaaaaaa cagaaacagt
    16381 gtcgtgaata aaaaaatact gatgagatca gtactcactg tcagtaaatt cacaatgcat
    16441 gctctcagag aaaattatta gcagaagtat aaccacgaag acctttattc agaagggaaa
    16501 gtccgttccg aacccaccag ccctgtgcaa aaatcaacca tttgatatgc aaattgcata
    16561 acatgccagc aaattccagg aattttccta acatcgccag cgcgacatta agattacaga
    16621 tataaaagca aaacgccacg taaacacgtg gcgtttttgg cataagacaa atttatgagc
    16681 gtttcatcat ttcgaagaaa tcgtcattgg tcttggtcat ggccagttta ttaatgagga
    16741 attccattgc atcgatttcg cccatcgggt gaatgatttt gcgcaggatc cacattttct
    16801 gcagttcttc ctgagtcgtg agcagctctt ctttacgggt accggaacgg ttgtagtcga
    16861 tagccgggaa gacgcgtttt tcagcgatct tacgagagag gtgcagttcc atgttgcctg
    16921 tacctttaaa ctcttcgtaa ataacttcgt ccattttaga accggtatcg ataagcgccg
    16981 tcgcgataat ggtcaggctg ccgccctctt ccacgttacg cgccgcacca aagaagcgtt
    17041 tcggacgatg aagggcattg gcgtccacac caccggtcaa cactttacct gacgccggaa
    17101 caacagtgtt gtaagcgcgc gccagacgag tgatggagtc gagcagaatg ataacgtctt
    17161 tcttgtgctc aaccaggcgt ttggccttct cgatcaccat ttccgcaacc tgaacgtggc
    17221 gagatgcggg ttcgtcaaag gtagaagcaa caacttcacc ttttaccaga cgctgcatct
    17281 cggttacttc ttccggacgt tcgtcgatca gcagaaccat cagcacacaa tccgggtggt
    17341 tgtaagcaat gctctgagca atgttctgca gcagcatagt tttaccggct ttcggcggtg
    17401 ccacaatcag accacgctga ccacgaccga taggtgatgc cagatccagt acgcgagcag
    17461 ttaaatcttc agtagaaccg ttaccacgtt ccatacgcag acgagagttt gcgtgcagcg
    17521 gggttaagtt ctcaaagagg attttgttgc gggcgttttc aggtttgtcg aagttaactt
    17581 cgttaacttt cagcagcgca aaatagcgtt caccttcttt cggcgggcga atcttaccag
    17641 agatggtatc accagtgcgg aggttgaaac ggcggatttg gctaggggaa acgtagatgt
    17701 catcaggacc ggcgaggtag gagctgtctg cggaacggag gaaaccaaat ccatcctgca
    17761 atatctccag tacgccatca ccaaagatat cttcgccact ctttgcgtgc tgcttcagga
    17821 tggcaaaaat aatgtcctgc ttacgcatgc gagccaggtt ttccagcccc atattttcgc
    17881 cgagagtgat cagctcagaa accggcgtat tctttaattc ggtaagattc atagtggtgt
    17941 gagttcttaa acttggggta aatctcgaac ttaatgttgt gaatggtatg gcaggatcat
    18001 ccatgcctgt ttacggtcat cgactcatgt cttttcgctg cctggtcaca ggagagtacg
    18061 cagaactgaa acgacaagac ggattgagtg acgagccaga aatctgttca cttcgcattt
    18121 aagataaaac gggaagcgtt ggaaattaca agattcaaac ttaataaggt atgtttaata
    18181 cgaagtcaac actaagttag catgactcac gccgggcgtc cagtttttag cgacggggca
    18241 cccgaacatg aaattccctt acgccaggtt agcgtcgagg aactctttca actgaccttt
    18301 agacagtgcg cccactttgg ttgccgccac ttcaccgttt ttgaacagca gcagagtcgg
    18361 gataccacgg atgccatatt tcggcgcagt gccagggttt tgatcgatgt tcagttttgc
    18421 aacggtcagt ttgccctgat attcgtcagc gatttcatcc agaatcgggg cgatcatttt
    18481 gcacggaccg caccactctg cccagaaatc gacgaggatc gccccgtccg ctttgagtac
    18541 atccgtgtca aaactgtcgt cagtcaggtg aataatttta tcgctcatat ataactccac
    18601 aggaataagc ctggcgtgtt ggtttcgttg ttggtgtaac attaaccaac taaaggttga
    18661 ctttatttca ccggatacgc tttcgtaaag caatagtaag ctgatattct accacactat
    18721 gagcaaaaca catttaacag aacagaagtt ttccgacttc gccctgcatc cgaaggttgt
    18781 agaagccctt gaaaaaaaag ggtttcataa ctgtacgccc attcaggcac tggcccttcc
    18841 gctgacgctg gcgggtcgtg acgtagccgg gcaggcgcaa accggtaccg ggaaaacgat
    18901 ggcgtttctt acgtcaacgt ttcattatct tctctctcat cctgcgattg ccgatcgcaa
    18961 ggtgaatcaa ccgcgtgcct taattatggc accgacgcgt gaacttgccg tgcagatcca
    19021 cgccgacgcg gaaccgctgg cggaagctac cggcctgaag ctgggcctgg cttacggtgg
    19081 tgatggctat gacaaacagc tgaaagtgct ggaaagcggc gttgacattc tgattggcac
    19141 cacgggtcgt ttaattgact acgccaagca gaaccacatt aacctcggtg ccattcaggt
    19201 agtggtactg gacgaagccg atcgcatgta cgatctaggc tttattaaag atatccgctg
    19261 gctgttccgc cgtatgccgc ctgcaaatca gcgcctcaac atgctgttct ccgccacgct
    19321 ttcgtaccgg gtacgtgaac tggcgttcga gcagatgaac aatgccgaat atattgaagt
    19381 ggaaccggaa cagaaaacgg gccaccgtat taaagaagag cttttctacc cttctaacga
    19441 agaaaaaatg cgtttgctgc aaacgctgat cgaagaagag tggccagaca gagcgattat
    19501 tttcgccaat accaaacacc gttgtgaaga gatctggggc cacctggcgg cagatggtca
    19561 tcgtgtcggt ttgttgacag gcgatgtcgc gcagaaaaaa cgtctgcgta ttcttgatga
    19621 atttacccgt ggcgatctgg atattctggt tgccaccgac gttgccgcgc gtggtttgca
    19681 tattccggca gtgacgcacg tctttaacta cgatttaccc gatgactgtg aagattacgt
    19741 tcaccgtatt ggtcgtacag gtcgcgcagg cgcaagcggt cactctatca gcctggcgtg
    19801 tgaagagtat gcattgaatt tgcctgctat tgagacctat attggtcact caattccggt
    19861 aagcaaatac aatccggacg cattgatgac cgatctgcca aaaccgctgc gcctcacgcg
    19921 cccgcgcaca ggcaatggtc cgcgtcgtac tggcgctccg cgtaatcgtc gtcgttcagg
    19981 ttaaataaaa tgtcatactc aaaatcattc aaactgtatc aagatggcaa gagaatgaat
    20041 cccgatgagc ttactgtagt aagtgattcg ggtgaaagaa tgcagccaac acagagacag
    20101 attgaaggat gaagagtatg ggttccacct cgtcgctgta tgcagccatt gatctcggtt
    20161 cgaatagttt tcatatgctg gttgtgcgcg aggtggctgg aagcatccag acgctgacgc
    20221 gaattaaacg caaagtgcgt ctggctgctg gcctgaatag cgaaaatgac ctctctaatg
    20281 aagcaatgga gcgcggttgg caatgtctgc gcctgtttgc tgaacgtctg caagatatcc
    20341 ctccctcgca aattcgcgtt gtcgctacgg cgacattacg acttgccgtc aatgcgggtg
    20401 attttattgc caaagcgcag gaaatcctcg gttgtccggt acaggtgatc agcggtgaag
    20461 aggaagcgcg tctgatttat cagggcgttg ctcacaccac tggtggtgcc gatcagcgcc
    20521 tggtggtgga tataggcggt gccagtactg aactggtaac cggcacgggt gcacaaacca
    20581 cctcgttgtt cagcctgtcg atgggctgcg tcacctggct ggaacgctat tttgccgatc
    20641 gtaatctggg gcaagaaaat tttgatgctg cagaaaaagc ggcacgcgaa gtgttacgtc
    20701 cggttgccga tgaattacgg tatcacggct ggaaagtgtg cgttggtgct tccggcaccg
    20761 tgcaggcgtt acaggaaatc atgatggcac aggggatgga tgaacgcatt accctggaaa
    20821 agttgcagca attgaaacag cgagccattc attgcggtcg gctggaggaa ctggagattg
    20881 acgggctgac gctggaacgt gcattagtgt tcccgagtgg tctggcgatc ctgatcgcca
    20941 tttttaccga actgaatatt cagtgtatga ccctggcggg cggtgcgctg cgtgaaggcc
    21001 tggtctacgg catgttgcat ctggccgtcg agcaggatat tcgcagccgt acactgcgta
    21061 atattcaacg ccgctttatg atcgatattg atcaggcaca gcgcgtagcc aaagttgcgg
    21121 ctaacttctt cgatcaggtg gaaaacgaat ggcatcttga agcaataagc cgcgatttgc
    21181 tcatcagcgc ctgtcagctt catgaaatcg gcctgagcgt tgacttcaaa caagctccgc
    21241 aacacgctgc ttatctggtg cgtaacctgg atcttcccgg ttttaccccc gcacagaaga
    21301 aactgctggc gacgctactg ctcaaccaga ctaacccggt cgatctctca tcgctgcatc
    21361 agcaaaatgc cgtaccaccg cgcgtcgcag aacaactctg ccgtttactc cgcctggcta
    21421 tcatttttgc cagccgtcgc cgtgacgatc tcgtgccaga gatgacatta caggctaacc
    21481 atgaactgtt gaccttgacg cttccgcaag gttggctaac ccaacatccg ctgggtaaag
    21541 agattattgc tcaggaaaac cagtggcaga gctatgtcca ctggccgctg gaagtgcatt
    21601 aatgagtaag tgccggatgc gatgctgacg catcttctcc ggccttgatt atttccctcg
    21661 ttttgccgcc atcatcgctt tcagattcgc cagatggctt tgccctttct gcatccgttc
    21721 ttcggcactg accactttgc gctcctgttc ccaaatcaga tcatcctgcg gcagctccag
    21781 caaaaagcgg ctcggctccg ggcgcaccag ttcgccgtac tgacggcgtt ctttacacag
    21841 cgtaaaggtc aattccttct gggcgcgggt aatgccgaca taggccagcc gccgctcctc
    21901 atcgatatta tcttcatcga tactgctctg gtgcggcaaa aacccttctt ccataccgac
    21961 catgtagaca taaggaaact ccagcccttt tgacgcgtgg agagtcatca gttgcacctg
    22021 atccagctct tcttcactct caccacgctc catcatgtcg cgtaaagtaa agcgcgtcac
    22081 cacctgggtg agcgtcatcg gctcatccag ttcgctgcct tccagcatct ccgtcatcca
    22141 gctaaacagt tggttgacgt tcttcatgcg catttcggca gctttcgtgc tgggcgatgt
    22201 ttcgtacagc caggattcat aatccatgcc gtggatcaga tcgcgcaccg cggcaatcgg
    22261 ctcccgctcc gccagacgct ggatttctgc caaccagtga gtgaagcggg tcaatgcttc
    22321 ataaccacgt ccgctaagcg tctgactcag gcccatatca aagctggcgg taaacatgct
    22381 tttattgcgc gtcatcgccc actcacccag ctttttcagc gtcgccgggc caatctctcg
    22441 cttcggcgtg ttaacgatac gcagaaatgc gctgtcatcg tccgggttag tcagcacgcg
    22501 cagataagcc agcaagtcct tgatttcagg acgagagaaa aacgacgtac caccagaaat
    22561 tttgtacggg atgcggtttt gcatcaggaa tttttcaaac acccgcgact gatagttacc
    22621 gcgataaaga atagcgtaat ctttgtactg cgttttattg acgaagtgat gcgcaatcag
    22681 ttcaccagtg acgcgctcgg cttcatgttc ttcgttattc gcgcttaaaa cttttagctc
    22741 tgtgccgtaa cccagttcgg agaacagacg cttttcaaag acgtgcgggt tattggcgat
    22801 caggatgttc gccgctttca aaatacgtcc ggaagagcgg tagttctgct caagcttaat
    22861 caccttcagc gccggaaaat cctggctcag cagcaccagg ttttgcggac gtgcgccgcg
    22921 ccaggagtag atcgactggt catcgtcacc caccacggta aagcgcgcgc ggctgcccac
    22981 cagcagtttc accagttcat actggctggt gttggtgtcc tgatattcgt ccaccagcag
    23041 gtagcgaatt ttgttctgcc agcgctcgcg gacttcttca ttgcgttgca gaagtagcgt
    23101 cggcaataaa atcagatcat cgaagtcgag cacattacag gctttcaggt gtgcatcata
    23161 cagcccataa caatgggcaa aaatacggtc ccgctcgcca atcgcactgg ctgccgcctg
    23221 ggacggtgtt ttgagatcgt tcttccagtt agagatggtc gaaatcaact gttgcaggag
    23281 aactttgtca tcttcaatca gcccttcggt caactctttg agcaacgcca gctgatcggt
    23341 atcgtcaaac agcgaaaagt tcgccttcat ccccagcgcc gcatactcac gcttgatgat
    23401 atccagcccc agcgtgtgga aggttgagat catcagccca tgcgcctctt tgcgacccag
    23461 cgtctgtccg acacgctctt tcatctcgcg cgccgcctta ttggtaaagg tcaccgccgc
    23521 gatatgtcgc gcctgataac cgcacccgcg gatcagatgg gcgattttat tggtgataac
    23581 acgagtttta ccggaacccg cacccgccag caccaggcag gggccggtaa cgaattcgac
    23641 agcttgttgt tggccggggt ttagacgcat aggaaaatcg ctcaatgaaa gtcgggaggg
    23701 gaaaataaca gcgtggtagt atagccagcc tcatccttac cactcaaggc acgatcatgg
    23761 caaaaacagc agcagcactg catatccttg taaaagaaga gaaactggct ctggatcttc
    23821 tcgtgcagat taagaacgat gccgatttcg gcaagctggc gaagaaacac tccatttgcc
    23881 catcaggcaa acgcggcggt gatttaggtg aattccgcca gggtcagatg gttccggcgt
    23941 tcgataaagt tgtgttctct tgtccggtac tggagccaac cggcccgctg cacacccagt
    24001 tcggttacca catcattaag gtgctgtacc gtaactaata gcaaggcctt ctccgggaga
    24061 aggccttgaa ctctgaatcg ctattaaccc gcaacagcaa tacgtttcat atccgtcata
    24121 tagccgcgca gtttcttacc tacttgctca atcgcatggc tgcgaatcgc ttcatttaca
    24181 tcacgcagct gcgcgttatc taccgcacct tccggaatag ctttacccaa atcgcccggt
    24241 tgcagttctg ccataaacgg tttcagcagc ggcacacaag cgtaagagaa cagatagtta
    24301 ccgtactcgg cagtatcaga gataaccacg ttcatttcat acagacgctt acggacgatg
    24361 gtgttggcaa tcagcggcag ttcgtgcagt gattcatagt aagcagattc ttcgatgatg
    24421 ccggaatcaa ccatggtttc gaacgccagt tcaacgcccg ctttcaccat cgcgatcatc
    24481 agtacgcctt tatcgaagta ctcctgctcg ccgattttgc cttcatactg cggcgcggtt
    24541 tcgaaggcgg ttttgccggt ctcttcacgc caggtcagca gtttcttatc atcgttggcc
    24601 cagtccgcca tcataccgga ggagaattcg ccggagatga tgtcgtccat atgtttctgg
    24661 aacagcggtg ccatgatctc tttcagctgt tcagacaacg cgtaagcacg cagtttcgcc
    24721 gggttagaga gacggtccat catcagtgtg ataccgccct gcttcagcgc ttcggtgatg
    24781 gtttcccaac cgaactgaat tagtttttct gcgtatgccg gatcggtgcc ctcttccacc
    24841 agcttgtcga agcacagcag cgagccagcc tgcaacatac cgcacaggat ggtttgctca
    24901 cccatcaggt cagatttcac ttccgcaacg aaggacgatt ccagcacgcc cgcacggtga
    24961 ccaccggttg ctgccgccca tgctttagcg atcgccatgc cttcgccttt cggatcgttt
    25021 tccgggtgaa cggcaatcag cgtcggtacg ccgaatccac gtttgtactc ttcacgcact
    25081 tcggtgccag ggcatttcgg cgcaaccatg actacggtga tgtctttacg gatctgctcg
    25141 cccacttcga cgatgttgaa accgtgcgag taacccagcg ccgcgccgtc tttcatcagt
    25201 ggctgtacgg tgcgcactac atcagagtgc tgcttgtccg gcgtcaggtt aaccaccaga
    25261 tccgcctgtg ggatcagttc ttcgtaagta cccactttaa aaccattttc ggtcgcttta
    25321 cgccaggacg cgcgcttctc ggcaatcgct tctttacgca gagcgtagga gatatcgaga
    25381 ccagaatcac gcatgttcag accctggttc agaccttgtg cgccacagcc gacgatgact
    25441 acttttttac cctgaaggta gctcgcgcca tcggcgaatt catcgcgccc cataaagcga
    25501 catttgccca gctgtgccag ctgctgacgc agattcagtg tattgaagta gttagccatg
    25561 gtgattcctc gtgatgttgt gcttcttatt gttcggttcg cgtttgcgag attgaattca
    25621 ctatatgaca ggaaatttat tgcggaaatt gatatattca caacgtcaca ttgcaatttc
    25681 tgcaacgtca acattgaggg ctgcgccatt ggatttacgc gatctgaaaa ccttcctgca
    25741 tctggcggaa agccgccatt ttggccgcag cgcgcgggcg atgcacgtta gcccgtccac
    25801 gctctcacgg cagattcagc gcctggaaga agatctcggc cagccgctgt ttgtgcgcga
    25861 taaccgcacg gtgacgctga ctgaagcggg cgaagaactg cgcgttttcg cccagcaaac
    25921 gctgttgcag tatcagcagc tgcgccacac catcgaccag caagggccgt tgctctatgg
    25981 cgaattacat atcttctgct cggtgaccgc cgcttacagc catctgccgc cgattctgga
    26041 tcgcttccgc gcggaacacc cgtcggtgga gatcaaactc actaccggtg atgcggcaga
    26101 tgcaatggaa aaagtcgtca caggtgaagc tgatctggcg attgccggga agccagagac
    26161 tctgcccggc gcggtggcgt tttcgatgtt ggagaatctg gcagtggtgt tgattgcacc
    26221 cgcattgccc tgcccggtgc gtaatcaggt ttctgtcgaa aagccggact ggtcaacggt
    26281 accgtttatc atggccaatc aggggccggt gcgccgccgc attgagttgt ggttccgacg
    26341 caataaaatc agtaacccga tgatttacgc cacggttggc gggcatgaag cgatggtatc
    26401 gatggtggcg ctcggctgtg gcgtggcatt gttgccggaa gtggtgctgg aaaacagccc
    26461 cgaaccggtg cgtaaccgcg tgatgatttt agagcgcagc gatgagaaaa cgccgtttga
    26521 gctgggcgtg tgtgcgcaga aaaagcggct acatgagccg ctaattgagg cattctggaa
    26581 gattttgccg aaccattaac ccgccaaaaa gaacctgaac gccgggttat tggtttcgtc
    26641 gtggcaatcg tagcccagct cattcagccg ggtttcgaaa tccggttcat ggtcgccaag
    26701 ttcgaacgcc gccagtacgc gcccgtagtc ggtgccatgg ctgcgatagt ggaacaaaga
    26761 aatgttccag tgcgtaccca gcgtgttgag gaagcgcagc agcgcgcccg gtgattccgg
    26821 gaattcgaag ctgtagaggc gttcctgcaa cggatgtgac ggacgcccgc cgaccatata
    26881 gcgcacatgc aacttcgcca tttcgtcgtc ggagagatcc accacgctgt agccgccgtc
    26941 gttgagcatc tgcaaaattt ctttgcgctc ttcgaggccg cggctcaggc gcacgccgac
    27001 aaagatgcag gcgtttttgg catcggcaaa acggtagttg aactcggtga ccgatcgccc
    27061 gccaagcagt tggcagaatt tgaggaagct gcctttttct tccggaatgg tcaccgccaa
    27121 caacgcttca cgctgttcgc ccagttcgca gcgttccgag acgtagcgca ggccgtggaa
    27181 gttcacgttg gcaccggaaa gaatatgcgc cagccgttcg ccgcgaatgt tgtgctgggc
    27241 gatatatttt ttcattcccg ccagcgccag cgcgccagag ggttccgcca ccgcacgcac
    27301 atcttcgaat aaatccttca tcgccgcaca gatcgcatcg ctatcgacgg tgatgatgtc
    27361 gtcgagatac tcctggcata aacggaaggt ttcgtcaccg atgcgtttta ccgcgacacc
    27421 ttcggcgaac agtcccacgc gtggcagatc aaccggatga cccgcatcca gcgctgcttt
    27481 caggcaggcg gagtcttccg cttctacggc gatcactttg atttgcggca tcagttgttt
    27541 gatcagcacc gctacgcccg ccgccagacc gccgccgccg actggcacaa atacgcggtc
    27601 gagatgggcg tcctgctgga gcagttccag cgccagcgtg ccttgcccgg caatcaccat
    27661 cggatggtcg aacggcggca cccaggtgaa cccctgctgc tgtgacagtt cgatcgcttt
    27721 agctttcgct tcgtcaaagt tcgcgccatg aagcagcact tcgccgccga atccacgtac
    27781 cgcatcgact ttgatgtcgg cggtggcggt tggcataacg atcagggcct tcacgcctaa
    27841 ccgcgcagaa gaaaacgcga cgccctgcgc gtggttaccc gcagaagcag tgatcacgcc
    27901 gtgcgctttt tgttcttccg tcaggcccgc catcatggcg tatgcgccgc gcagcttaaa
    27961 gctgtgcact ggctggcgat cttcgcgctt caccagaatg acgttatcaa gacgcgacga
    28021 cagtttttcc attttttgta gcggcgtaac ctgcgccgcc tcgtaaaccg gcgcgcgcag
    28081 cactgctctt aaatattcgg caccttccgg agcaccggac aggggttgcg agtcagccat
    28141 tattaacccc ccagtttcga tttatcgcgc accgcacctt tgtcggcgct ggttgccagg
    28201 ctggcgtagg cgcacagggc aaaggaaacc tgacgttcac ggtttttcgg cgtccaggct
    28261 ttgtcacccc gagcttcctg cgcttcacga cgcgccgcca gttcggcatc gcttacctgt
    28321 aactgaatgc cacggttcgg gatgtcgata gcgatcaggt caccatcttc aatcagacca
    28381 atgctgccgc cgcttgccgc ttccggtgag acgtggccga tggaaagacc agaggtgccg
    28441 ccagagaaac gaccgtcggt gatcagcgca caggctttac cgagacccat tgatttcagg
    28501 aagctggttg ggtagagcat ttcctgcatg ccagggccgc ctttcgggcc ttcatagcgg
    28561 atcaccacca catcgcccgc gacaactttg ccaccgagga tcgcttctac cgcgtcgtcc
    28621 tggctttcgt acactttcgc cgggccggtg aatttgagga tgctgtcatc gacgcccgcg
    28681 gttttcacga tgcagccgtt ttcagcaaaa ttaccgtaga gcaccgccag gccgccgtct
    28741 ttgctgtagg cgtgttccag cgagcggata cagccgtttg cgcgatcgtc atcgagagaa
    28801 tcccaacggc aatcctgcga gaatgcctgt gtagtacgaa tgcccgcagg gcctgcgcgg
    28861 aacatatttt ttaccgcgtc atcctgggtc agcataacgt cgtattgttc cagagtttgc
    28921 ggcaacgtca ggccaagtac gtttttcaca tcacggttca gtaaccccgc gcgatccagt
    28981 tcaccgagaa taccgataac accaccagca cggtgaacat cttccatatg gtatttttgg
    29041 gtgctcggcg caactttgca taactgcggg actttacgcg aaagtttgtc gatatcactc
    29101 atggtgaagt cgatttccgc ttcctgcgcc gccgccagca ggtgaagtac cgtgttggta
    29161 gagccgccca tcgcgatatc cagcgtcatg gcattttcaa acgccgcttt gctagcaata
    29221 ttgcgcggca gtgcactttc gtcgttttgc tcgtagtaac gtttggtcaa ttcaacaatg
    29281 cgtttaccgg cattgaggaa caactgctta cggtccgcgt gggttgccag cagcgagccg
    29341 ttgcccggct gcgacaggcc cagcgcttcg gtcaggcagt tcattgagtt agcggtaaac
    29401 atcccggagc aggagccgca ggtcggacac gcggaacgtt caacctgatc gctctgggag
    29461 tcagaaactt tcgggtctgc gccctggatc atcgcatcaa ccagatcgag tttgattatc
    29521 tgatcggaca gtttggtttt cccggcctcc atcgggccgc cggaaacaaa gatcaccgga
    29581 atattcaggc gcagggaagc catcagcatc cccggggtga ttttgtcgca gttagagatg
    29641 cagaccatgg cgtcggcgca gtgggcgttg accatatact caacggaatc agcgatcagt
    29701 tcgcgagatg gaagtgaata aagcatcccc ccgtggccca tggcaatccc atcatccacc
    29761 gcaatggtgt tgaactcttt ggcaacgccg ccagccgctt caatttgttc ggcgaccagt
    29821 ttaccgagat cgcgcagatg gacgtgaccc ggtacaaatt gggtgaacga gttcacaacc
    29881 gcgataatcg gcttgccaaa atcggcgtcg gtcattccgg tggcgcgcca cagcgcacga
    29941 gcacccgcca tattacgacc atgagtggtg gtggcggaac ggtacttagg catactttat
    30001 ttactcccag tgtctgtctc gtaaatggga cggtgcgtgc cgtcccattt tttgtattta
    30061 ttgattaact tgatctaacc agccccattt atcttcggtt tcgccagtga agaggccgaa
    30121 gaaggcttgc tgaatgcgtt tggtaaccgg gccacaacgg ccttcgccaa tctgaatacc
    30181 gtctacgctg cgcactggcg tgatttctgc cgccgtaccg gacataaaga cttcatccgc
    30241 caagtacagg gattcgcgcg acagcacctg ctcacgcact tcaattccca gctctttcgc
    30301 cagtttgatg atggcatcac gggtaatacc cggcagcgcg gaggaggtga acggtggggt
    30361 gaacagcaca ccatctttca cttcaaacag gttttcgcct gcgccttcag agatataacc
    30421 gttcacatcc agcgcgatac cttcctgata accgtggcgg cgcgcttcgc tacccaccag
    30481 cagggaagag agatagttac caccggcttt tgccgcagtt gggatggtgt ttggtgctgc
    30541 gcggttccag gaggaaacca tcgcatcgat cccctgctcc agcgcttctg cgcccagata
    30601 cgctccccac gggaaagcgg cgataatcac atcggttgag tatcccgctg gcgggtttac
    30661 tcccatgcca acatcaccga cgaagatcag cggacggata taggcgctgg tgagattgtt
    30721 tttgcggatc acgtcacgac aagcttccat cagctcatca atgctctgtg aaaccgggaa
    30781 gcgatagatt ttggcggagt catgcagacg ctgcatatgc tcacgatggc ggaatacaac
    30841 cggtcctttg tgtgagtcgt agcaacggat gccttcaaaa accgaggtgc catagtgcag
    30901 cgcgtgcgac atcacatgca ccttcgcgtc ttcccagcga accatctccc cattgaacca
    30961 aatgtaatca gctttcttcg tggtcatttt tatattcctt ttgcgctcag gcgcggattt
    31021 gttgtgatgt ggttgtgctc tggcagatgg caacgtgtgc gacgtccacc agtttattta
    31081 actgactaaa cagtaagtcg accgaccgtg ggctggcaac ggtcaattcg atatttatat
    31141 tttgtgcatc gctggcggca gccatattca ttgagcagac gtggaaacca cgatgacgca
    31201 ccacgcgtaa aacacgttct aaggtttccg gattgaagcg agccgataca ttgacctgat
    31261 gttgcatcat gataatttct ccaacatttc tgaattactg gcacctggcg gcaccagcgg
    31321 ccagacgttc tcaagttcgt cgattgagac atgaagcagg tatggcccat cactgttcag
    31381 catggtgtcg agtgccgctt caacctggtc tttacgggtg atgtgttggc cagggatgcc
    31441 gaaggcgctg gctaacatga ggaaatcggg gttatcggta agggtggttt cgctgtatcg
    31501 ttcctgaaaa aacagttgct gccattgtcg aaccatccct aaccgttggt tatcgagtaa
    31561 gacgattttc aacggtaact gcttgcgttt tacggtgccc agctcttgca cattcatcat
    31621 gaaagagccg tcaccggaga tacagacaac ggtatcgttc ggtcgcgcga cttgtgcgcc
    31681 aactgccgcc ggtaaaccaa aacccatggt acctaagccg ctggaggtga tgaaattttc
    31741 cgggcgagtg tgggcgatgt gctgcgcagc ccacatctgg tgctgcccca catctgtggt
    31801 cacgacgcaa tccgcaggtt tacgatccga cagttgtttt aacaacaacg gagcgtagat
    31861 agcgtcaccg ggatggtcgt aacgccagga atgttcatca cgcagctgcg cgcagtgttg
    31921 ctgccagtca ttgatattta acggctgctg taatgctggt aacagagcat ttaaatcacc
    31981 ttgtaatgcc acatgtgcct gacgcagctt gttcatttct gccgggtcga tatccatatg
    32041 gataacactg gcgtgtggtg cgaaggtgtt cagtttgccg gtcacccggt catcaaaacg
    32101 tgcacccacg gcgatcagca ggtcacactc ctgcaccgcg aagtttgccg ctttggttcc
    32161 gtgcatcccc agcatgccca gatagtacgg ataatctgct tctactgcgc ccagcccttt
    32221 cagcgtacag gtggcaggca tttttgtggc agcgagaaat tcacgcaaag ccggaactgc
    32281 ctgcgccatc cccacgccac cgccaacgta cagcatcggt ttttgcgctt ttgccagcat
    32341 ctggcgcgct tgctcgactt cggcatgtgg gaaagtcact tcgttttcaa cggtggtgaa
    32401 ccacggttcc agatcgccgc tggctaactg gatatctttt gggatatcga ccagaaccgg
    32461 accaggacga cctgagctgg caacgtcgaa tgcttcagcc atgatgcgcg gcaactcttc
    32521 cagagactgc accagaaagc tgtgcttggt acaggctaac gacaatccca ggacatccac
    32581 ttcctgaaat gcgtcggtgc cgataaacgg tgcggacacc tgaccagtga tggcaacgac
    32641 agggatggaa tctaacagtg cgtccgcaag cccggttatc aggttggttg cgcccggacc
    32701 agacgtggcg atacatacgc cagttttacc ggtagcacgg gcataaccga tagccgccat
    32761 tgccgcaccc tgctcatgtc ggcacagcaa gtgctccacg ccgccgtcat acaatgcatc
    32821 gtaaaccggc ataattgcgc cacccggata accgaaaacg gtgttcacac cctgtgcccg
    32881 caacgcatgt accacccact gtgcgccatt catagttagt tccccgtcct gaatcttgag
    32941 aaacagaatt ttgtgctgtt attcattgtc tgctcctcgg ttatgttttt aaggtcaaaa
    33001 aaacccccgg acctttcggt gcgggggtct tagttcgtta aggcttgatc tctaagcctt
    33061 tcctcgtcca agtgcagccc cgcacggtgg gataataatc accaccacgc taatcacgac
    33121 caggctaatc actcgtagaa gggctgtcat tttgtctttt cttgcatctt gttcgaagga
    33181 atgcctaaag agttaccata gattttgcga atagcacaag atatttttgg gcctattttt
    33241 atgatggctt taataatttc aatagaaatt gttgtttttt aaaaagaaaa tagtgagtga
    33301 aaaaattcaa tttcagtata aatgttgttc ccttcctcgt agttcatcct ttgtccgctg
    33361 catttttgcg agcatcattc caccgccaga acaactcaac ttcacttgca caaataaaca
    33421 ggaatcagcc actaaaattc acgaattagc gggctacagc agcgatatct tatgcacata
    33481 atggtgactc aaggagggtt tatgtcactg tcaattgttc atacccgcgc agccctggaa
    33541 gtaaatgcgc ccccaatcac tgttgaggta catatcagta aaggtctacc cggcttaacg
    33601 atggtgggct taacagaaac aacggtgaaa gaagctcgcg atcgcgtgcg cagcgccatt
    33661 atcaatagcg gatatgaata tccggcgaaa aaaatcacca tcaaccttgc tccagccgat
    33721 ctgccaaaag aagggggacg atatgattta cctatcgcca ttgcgttgct ggcggcctca
    33781 gaacagctta cagccaataa gttagatgaa tatgaattag tcggagaact agcgcttaca
    33841 ggcgctctgc gtggcgttcc cggtgcaatc tccagtgcaa ctgaagctat taagtcgggc
    33901 agaaaaatta tcgtcgcgaa agataacgaa gatgaagtgg ggctaattaa cggtgaagga
    33961 tgcctgatag ccgatcatct gcaggctgtc tgtgcgtttc tggaaggtaa gaacactctc
    34021 gaacgcccga aaccaactga tgcagtatcc cgggcgctac aacatgatct cagtgatgtt
    34081 gtcggtcagg agcaaggaaa acgaggactg gaaattaccg ccgctggtgg gcacaacctt
    34141 ttactgattg ggccgccggg aacaggtaaa acaatgctcg ccagccgtat taatggcctg
    34201 ttgccagatt taagcaatga agaggcactg gagagcgctg cgatattaag tctggtaaat
    34261 gctgaatcag tacaaaaaca atggcggcag cgcccgttcc gctcacctca tcacagtgcg
    34321 tcgttaactg cgatggtggg cggtggtgca attccagggc caggtgaaat ttcgttggcg
    34381 cataacggcg tgctttttct tgatgagcta cctgaatttg aacggcgtac actggatgcc
    34441 ttgcgagagc cgattgaatc cgggcagatc catctttcac gcacacgagc aaaaataacc
    34501 tatccagccc gtttccagct tgttgcggcg atgaatccca gccctaccgg acattatcag
    34561 ggaaaccata accgctgcac gccagaacag acattacgtt atctcactgg agatcccatt
    34621 accacccccc ggcattttga gtaaaacggt agtgccggga gaaagcagcg ccaccgttaa
    34681 acaacgcgta atggccgcca gagagcgcca atttaagcgg cagaataaac tgaatgcctg
    34741 gctggatagt ccggaaatac gccaattctg caaacttgag agcgaagatg cgatgtggtt
    34801 ggaagaaacg ctgatccatc tggggttatc gattcgtgcc tggcagcggt tactgaaagt
    34861 tgcacgaacc attgctgata ttgatcagtc tgacattatc acacgtcagc atttgcagga
    34921 ggcagttagc tatcgtgcga ttgaccgttt gctcatccat ctgcaaaaac tactgacata
    34981 aaaaaagggc atttcgccct ttttattaat cgtcagaatc ggtgtagtct tcagcacctt
    35041 caacctgcgg tttaccgccg gaaagggtgt gaaaacgttt tggacgctta atacgcgtca
    35101 tatacttgga ccacacgcgt tctgcttctg tcactggctc acgttcgcca cggcatactg
    35161 ctacaaagag tttctcttcc tcggtaaccg gctcgcgttt gccaagatcc aactcattga
    35221 aggcataacc atgacgctca agcagttgtg cctctttgat ggtgaaatca ccatgacgag
    35281 agaatccacg tggataatgt ttattgtcga aatatcgatt agtcgtcgta aagctttccg
    35341 ccatcctgca cgctcctaat tctttgaccg agctagttat ggcgcggagt attagttacg
    35401 cttgacagag tgtaaaacaa aacatttaaa tcataacgac aaataatttt gcggagagca
    35461 ctgtggatac ggaattgtta aaaactttcc tggaagttag ccgaacgcgt cactttggtc
    35521 gagcggctga atcgctctat ctgacccagt cagcagtgag ctttcgaatc agacaactgg
    35581 aaaatcaact gggtgtgaac cttttcaccc gccacagaaa caatatccgt ttaaccgctg
    35641 ccggtgaaaa actactgcct tatgcagaaa cgctcatgag cacctggcag gccgcccgca
    35701 aggaggtggc gcatacctca cgacataacg agttttctat cggtgccagc gcctcgttgt
    35761 gggaatgtat gcttaatcag tggttgggac gcttgtatca aaatcaggat gcccatacag
    35821 gcttacagtt cgaagcgcga attgcccaac ggcagtctct ggtaaaacag ctgcatgaac
    35881 gccagcttga tcttcttatt accactgaag cgcccaaaat ggacgaattt agtagtcagt
    35941 tgctggggta tttcacttta gcgctttata ccagtgcccc ttctaaacta aagggagatc
    36001 ttaactatct gcgactggag tgggggccag attttcaaca gcatgaggca ggtttgatcg
    36061 gtgctgacga agttcccatt ctgacaacca gttctgctga actggcacag caacagattg
    36121 cgatgcttaa tggttgcacc tggctacccg tcagctgggc gcgtaaaaaa ggcggcctgc
    36181 ataccgttgt cgatagcaca acactttcac ggccgcttta tgccatatgg ctgcaaaata
    36241 gcgataaaaa tgcgttgatt cgcgatcttt tgaaaattaa cgtgctggat gaagtgtatt
    36301 aatctgaatg gctggcaagg atgccggtag aaggatttac ttcggagagg attatttcag
    36361 ataaaaaaaa tccttagctt tcgctaagga tgatttctgg caggggcgga gagactcgaa
    36421 ctcccaacac ccggttttgg agaccggtgc tctaccaatt gaactacgcc cctaattagg
    36481 gtggcggaac ggacgggact cgaacccgcg accccctgcg tgacaggcag gtattctaac
    36541 cgactgaact accgctccac cgaattcttt tacaaccacc ggtttta
//
LOCUS       DAEBXN010000031        35755 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.031, whole genome shotgun sequence.
ACCESSION   DAEBXN010000031 DAEBXN010000000
VERSION     DAEBXN010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35755
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.031"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..246
                     /locus_tag="KJL99_001606"
     CDS             <1..246
                     /locus_tag="KJL99_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HBD4436368.1"
                     /translation="VTIPRGQLKNLKASYTLTEPQLTAPLKKGQVVGTIDFQLNGKSI
                     EQRPLIVMENVEEGGFFGRMWDFVMMKFHQWFGSWFS"
     gene            complement(293..1051)
                     /gene="deoR"
                     /locus_tag="KJL99_001607"
     CDS             complement(293..1051)
                     /gene="deoR"
                     /locus_tag="KJL99_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HBD4436369.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1109..1705)
                     /gene="ybjG"
                     /locus_tag="KJL99_001608"
     CDS             complement(1109..1705)
                     /gene="ybjG"
                     /locus_tag="KJL99_001608"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBD4436370.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            1990..3216
                     /gene="mdfA"
                     /locus_tag="KJL99_001609"
     CDS             1990..3216
                     /gene="mdfA"
                     /locus_tag="KJL99_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HBD4436371.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISFCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETAMRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARL
                     TSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGLVR
                     LTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWLSL
                     MVIFLKDKQMGNSHEG"
     gene            complement(3257..3541)
                     /locus_tag="KJL99_001610"
     CDS             complement(3257..3541)
                     /locus_tag="KJL99_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4436372.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(3627..4442)
                     /gene="ybjI"
                     /locus_tag="KJL99_001611"
     CDS             complement(3627..4442)
                     /gene="ybjI"
                     /locus_tag="KJL99_001611"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HBD4436373.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVMSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGYNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(4442..5650)
                     /locus_tag="KJL99_001612"
     CDS             complement(4442..5650)
                     /locus_tag="KJL99_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD4436374.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     SYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            5734..6270
                     /gene="rcdA"
                     /locus_tag="KJL99_001613"
     CDS             5734..6270
                     /gene="rcdA"
                     /locus_tag="KJL99_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HBD4436375.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(6445..8130)
                     /locus_tag="KJL99_001614"
     CDS             complement(6445..8130)
                     /locus_tag="KJL99_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HBD4436376.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLHHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            8400..8777
                     /locus_tag="KJL99_001615"
     CDS             8400..8777
                     /locus_tag="KJL99_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HBD4436377.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(8807..9064)
                     /gene="grxA"
                     /locus_tag="KJL99_001616"
     CDS             complement(8807..9064)
                     /gene="grxA"
                     /locus_tag="KJL99_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HBD4436378.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            9224..9511
                     /locus_tag="KJL99_001617"
     CDS             9224..9511
                     /locus_tag="KJL99_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HBD4436379.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNCPPQYSRSEREKDNDANH"
     gene            9495..10217
                     /gene="nfsA"
                     /locus_tag="KJL99_001618"
     CDS             9495..10217
                     /gene="nfsA"
                     /locus_tag="KJL99_001618"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HBD4436380.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRWDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            10278..11180
                     /gene="rimK"
                     /locus_tag="KJL99_001619"
     CDS             10278..11180
                     /gene="rimK"
                     /locus_tag="KJL99_001619"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HBD4436381.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIYYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGYFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            11268..11744
                     /locus_tag="KJL99_001620"
     CDS             11268..11744
                     /locus_tag="KJL99_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HBD4436382.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            12096..13208
                     /gene="potF"
                     /locus_tag="KJL99_001621"
     CDS             12096..13208
                     /gene="potF"
                     /locus_tag="KJL99_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HBD4436383.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYVNANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            13303..14436
                     /gene="potG"
                     /locus_tag="KJL99_001622"
     CDS             13303..14436
                     /gene="potG"
                     /locus_tag="KJL99_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HBD4436384.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEISSRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            14446..15399
                     /gene="potH"
                     /locus_tag="KJL99_001623"
     CDS             14446..15399
                     /gene="potH"
                     /locus_tag="KJL99_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HBD4436385.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            15396..16241
                     /gene="potI"
                     /locus_tag="KJL99_001624"
     CDS             15396..16241
                     /gene="potI"
                     /locus_tag="KJL99_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HBD4436386.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     GGWSTRWYGELLRDDAMMSAVGSSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            16301..16789
                     /locus_tag="KJL99_001625"
     CDS             16301..16789
                     /locus_tag="KJL99_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HBD4436387.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            16830..17957
                     /gene="rlmC"
                     /locus_tag="KJL99_001626"
     CDS             16830..17957
                     /gene="rlmC"
                     /locus_tag="KJL99_001626"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HBD4436388.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKTLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(18132..18863)
                     /gene="artJ"
                     /locus_tag="KJL99_001627"
     CDS             complement(18132..18863)
                     /gene="artJ"
                     /locus_tag="KJL99_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HBD4436389.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVISKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(19155..19823)
                     /gene="artM"
                     /locus_tag="KJL99_001628"
     CDS             complement(19155..19823)
                     /gene="artM"
                     /locus_tag="KJL99_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HBD4436390.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(19823..20539)
                     /gene="artQ"
                     /locus_tag="KJL99_001629"
     CDS             complement(19823..20539)
                     /gene="artQ"
                     /locus_tag="KJL99_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HBD4436391.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(20546..21277)
                     /gene="artJ"
                     /locus_tag="KJL99_001630"
     CDS             complement(20546..21277)
                     /gene="artJ"
                     /locus_tag="KJL99_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HBD4436392.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEAPYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(21295..22023)
                     /gene="artP"
                     /locus_tag="KJL99_001631"
     CDS             complement(21295..22023)
                     /gene="artP"
                     /locus_tag="KJL99_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HBD4436393.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(22241..22756)
                     /locus_tag="KJL99_001632"
     CDS             complement(22241..22756)
                     /locus_tag="KJL99_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBD4436394.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATQLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDTRNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            23405..25174
                     /locus_tag="KJL99_001633"
     CDS             23405..25174
                     /locus_tag="KJL99_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBD4436395.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNPSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENIPSANYQFNTFFSNTFEIDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQEQLDKYFTIESHPLLYRYT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKDAFIHFYDYSRRYAISENIDKDAVTNNIA
                     LMSTFYDSDNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMHSSHYHDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGCFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNVINSAFKNISFRSVKINNVNTHSLKFINCNFFNVDMIRVNLSKCLFH
                     ECSMHGVKIKPWLPVKWTKELISDYLYGCLLSLYSICARDIYNMNAGNNVKVAADAFL
                     EIIFSLKNKYCIKLLSAQDRAFIYEFARMIFAYINDKSIEILLLSCFAAADQKAIQRY
                     RPQSQDGEDFRSHLQYKLPLSAH"
     gene            25385..25708
                     /locus_tag="KJL99_001634"
     CDS             25385..25708
                     /locus_tag="KJL99_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HBD4436396.1"
                     /translation="MQFSTTPTLEGLTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            25705..26535
                     /gene="amiD"
                     /locus_tag="KJL99_001635"
     CDS             25705..26535
                     /gene="amiD"
                     /locus_tag="KJL99_001635"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HBD4436397.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRHQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLIPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(26532..27545)
                     /locus_tag="KJL99_001636"
     CDS             complement(26532..27545)
                     /locus_tag="KJL99_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBD4436398.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRASGRNEAMGKLLEKMG
                     AEFVPTDLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRNIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGASKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(27644..29074)
                     /locus_tag="KJL99_001637"
     CDS             complement(27644..29074)
                     /locus_tag="KJL99_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HBD4436399.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQLALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWLVVNQIGGKERYFFGNILWQTRALMDRAIGHNLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(29085..30086)
                     /gene="ltaE"
                     /locus_tag="KJL99_001638"
     CDS             complement(29085..30086)
                     /gene="ltaE"
                     /locus_tag="KJL99_001638"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HBD4436400.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALENNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(30123..31841)
                     /gene="poxB"
                     /locus_tag="KJL99_001639"
     CDS             complement(30123..31841)
                     /gene="poxB"
                     /locus_tag="KJL99_001639"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HBD4436401.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNNVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVMPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(31974..32942)
                     /gene="hcr"
                     /locus_tag="KJL99_001640"
     CDS             complement(31974..32942)
                     /gene="hcr"
                     /locus_tag="KJL99_001640"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HBD4436402.1"
                     /translation="MTMPTNQCPWRIQVHHITQETPDVWTISLICHDHYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLADVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(32954..34606)
                     /gene="hcp"
                     /locus_tag="KJL99_001641"
                     /gene_synonym="priS"
     CDS             complement(32954..34606)
                     /gene="hcp"
                     /locus_tag="KJL99_001641"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HBD4436403.1"
                     /translation="MFCVQCEQTIRTPAENGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLNA"
     gene            complement(34750..35649)
                     /gene="lysO"
                     /locus_tag="KJL99_001642"
     CDS             complement(34750..35649)
                     /gene="lysO"
                     /locus_tag="KJL99_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HBD4436404.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKAINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
BASE COUNT         8910 a         8838 c         9189 g         8818 t
ORIGIN      
        1 gtgaccatac cgcgtgggca gctgaaaaac ctgaaggcga gctacacgtt aacggaaccg
       61 cagcttaccg caccgctgaa aaaaggtcag gttgtcggga ccattgattt ccagcttaac
      121 ggtaaatcca ttgagcagcg tccgctgatc gtgatggaaa atgtggaaga gggcggattc
      181 tttggtagga tgtgggattt cgtgatgatg aaattccatc agtggttcgg cagctggttc
      241 tcttaatctt ctgataaccg gatggcgcga aacgtcatcc ggttatacgt cattaataca
      301 tcaacttaat acggtgcgtc tgcgcgtact tcacatactc atcttccggg caacaatcac
      361 tcaccacaat atcaaagcgt ttcaggtcac ccatgcgcgc cggacgcacc ttgccaaatt
      421 tactgtggtc gacaaccagc acatgctttt gcgccatcga catggcccag tgttttaccg
      481 gcaactcttc aagattaaaa caggtagcgc ctttactgac atgcacgccc gccgcggaat
      541 aaaaagcgat atccgggcaa aaattattca acgtttgctg aaaatcgatg ggtttgaaaa
      601 tggcgttgct ggcgtgaaat tcaccaccgc acagaaatgc gcggcaatgg ggtttctctt
      661 tcagcgccag aaaggtattt agcgaataac aaacggcggt aaaagggatt tcattatcaa
      721 tcgcttcaat aatccacggc gtggtggtgc cacagtcaaa aaagagggtc tgatcgggtt
      781 ctaccagtgt cgcagccagt tttgccgccc ggcgtttttc ttccaccagg cgggattttt
      841 gatcgcttaa caggtaatgg ctggcactgc gcggttccag aacaatatag ccgccgagca
      901 aaacgacggg cgcactgtgg ttgttcagat cgcgacgaat cgtcatctcc gaaaccccaa
      961 gcagggcggc ggcgtcttta agatgtaact tatcgctgcg ttttaattct tgcagcagct
     1021 gcccgatacg ctcttcgcga cgtgtttcca taatccctct gaatagttat tgaagcgagc
     1081 cgctcaatac tacacttttt agcagagatc agtcacgcac ccagcctttg cggatcggta
     1141 atgcaaaaca gacgcgatac cacgattgca gacgttgata gagtttatgc cccatcgctt
     1201 gccagataat ctgggcacta aggcagccaa tcatacctgc cagcaatcca ccgagcatat
     1261 ccagcggcca gtggacgcca agataaacgc gcgaccaggc aatgacgacg gccagcacca
     1321 ttaaaagtga gccggaccac aggcgatgcc agcataaaaa tgccagtgca aaggtgaaaa
     1381 tcaccgtacc gtgatcgctc gggaatgagt catccgccgc atgatgcagg aagttatagc
     1441 cgatattttc gacaaagggt cggtcgtgcg gaaaaagatg tcccatcgtc caggacacaa
     1501 acaggctgac cgccagcgcg atagcgattt ttatcaccag ttgccgttgt gctgtaagcc
     1561 cccacaacca aagtaccgcg gccagcaacg gcaccacggt aatcaaatct ttagcaataa
     1621 aaatcgccaa cgagatcatc cacggagccg agtctggcgt cgcgttaata agagagaata
     1681 gagagagatt caaattttcc agcataactt cccgacgcaa agtgattaaa agggagccaa
     1741 tacaggcaag tcgttgagaa taaagtgcag gttaaactgg gtaaagcggc atcgtcttat
     1801 ttccctcaag cggcctgttt acggtgggtg attgtaacgg gcataggtta aataaaactt
     1861 aaagaaagcg tagctatact cgcaataatg taagaatgtg cttaaccgtg gtttcagcta
     1921 caaaattcgc tttctcgtta gctgcgcttt tattaaactc tgcgcgatta ttattggcga
     1981 agaaattgca tgcaaaataa attagcttcc ggtgccaggc ttggacgtca ggcgttactt
     2041 ttccctctct gtctggtgct ttacgaattt tcaacctata tcggcaacga tatgatccaa
     2101 cccggtatgt tggccgtggt ggaacaatat caggcgggca ttgattgggt tcctacttcg
     2161 atgaccgcgt atctggcggg cgggatgttt ttacaatggc tcctggggcc gctgtcggat
     2221 cgtattggtc gccgtccggt gatgctggcg ggtgtggtgt ggtttatcgt cacctgtctg
     2281 gcaatattac tggcgcaaaa cattgaacaa ttcaccctgt tgcgcttctt gcagggcata
     2341 agcttctgtt tcattggcgc tgtgggatac gccgcaattc aggaatcctt cgaagaggcg
     2401 gtttgtatca agatcaccgc gctgatggcg aacgtggcgc tgattgctcc gctacttggt
     2461 ccgctggtgg gcgcggcgtg gatccatgtg ctgccttggg aggggatgtt tgttttgttt
     2521 gccgcattgg cagcgatctc ctttttcggt ctgcaacgag ctatgcctga aaccgccatg
     2581 cgtataggcg agaaactgtc actgaaagaa ctcggtcgtg actataagct ggtgctgaag
     2641 aacggccgct ttgtggcggg ggcgctggga ttcgttagcc tgccattgct ggcgtggatc
     2701 gcccagtcgc cgattatcat cattaccggg gagcagttga gcagctatga atatggcttg
     2761 ctgcaagtgc ctattttcgg ggcgttaatt gcgggtaact tgctgttagc gcgtctgacc
     2821 tcgcgccgca ccgtacgttc gctgattatt atgggcggct ggccgattat gattggtctg
     2881 ttggtcgctg ctgcggcaac ggttatctca tcgcacgcgt atttatggat gaccgccggg
     2941 ttaagtattt atgctttcgg tattggtctg gcgaatgcgg gactggtgcg attaaccctg
     3001 tttgccagcg atatgagtaa aggtacggtt tctgcggcga tgggaatgct gcaaatgctg
     3061 atctttaccg tcggtattga aatcagcaaa catgcctggc tgaacggggg caacggactg
     3121 tttaatctct tcaaccttgt caacggaatt ttgtggctgt cgctgatggt tatcttttta
     3181 aaagataaac agatgggaaa ttctcacgaa gggtaaaaaa atgcctgatt gatttgtgcg
     3241 atcaggcatt ctcgaattaa tggtgatggt cgtcaatctg gtgttcgata accatccctt
     3301 cacctacgct ggcaagatgg cgaacataag gatgcgggcg gtaagccgga gctggcgcag
     3361 gagccacata gacggtttgc ggcacagtcg cgacgctgac cgcttgtgga acgctgactg
     3421 aatcaggaac cacaaccact ttgtatccac tcggcacatc gacggtgaca ctttgcgcca
     3481 tcgccacacc agtaaagccc attaaaagcg cgcccaacag tagacaattt ttcataatca
     3541 tttcgcctga aataatgcag cgggttaatt tccgcgaatt atgcagcctt cattcaggca
     3601 ttgtgttgcc gggcgtgctg ccgtttttat tggtcaaatg gcgcttcgtg tttaagaact
     3661 ttatcgatca catccagtac gccttcacgg ttattgtagc ctgcccggta ttttgccgct
     3721 gcgacgactg cgctgccggc attttccatt gcaaaactaa agcctgcctg acgcagcatc
     3781 tcaatatcgt taccgccatc gccaaagacc accacttcgc tgtcgtctat tccccataat
     3841 ttctgcagtt ggcgaaggcc attggcttta tgtacgccgg ggataatcag atcgatgctg
     3901 ccgttgccgg tgtggactgg caccataata tcgccgatgg cctcatgtaa tgctttttgt
     3961 acttgtggaa tcagttcatc ggaaagattc aggccaaact taaagaaaat atcttctaag
     4021 ttgtcaaagt tatcgacgta ttccagacgg tgataataca tttccgccac cgttttcatg
     4081 gcatcgtcat attttttgag tgtataggcg ctattttttc cgcaggcaat aatttccact
     4141 tccgggcgcg tcagcaaatg ttccacgaca gtagcaaacg catccttcga cagctcgcca
     4201 ttaaaaacat ctttgccttc actcattacc cagccgccgt tttcagccac aaaggcaatt
     4261 tcattcgcaa tttcagggaa gaaagagatc aactgataat attgattccc gctggcgacc
     4321 acaaagcgaa tgccttgtgc tttcatttgc tgatactgag ccataaaccg ctcacggtta
     4381 taggtttttt gatcgcttaa gaaagtacca tccatgtcta ccgcaattaa tttaatgctc
     4441 atcaactatt ctccatcgcc gtctgcgttt tggtatcggg tttggcgacg gctttcgcga
     4501 caatagccgc gagaataacc agcgccagta caaccagcat tgcactacgt aatccataat
     4561 gttcgccgag atagcccagc agcggcggcc cgacgaggaa agccagataa ccggtcgttg
     4621 ctaccacact gacgcgggtt ggtgcatcgg ggccggtatc gctggcggca gaaatggtca
     4681 gcgggaagcc cagcgaggca cccagtcccc aaagtacaac agacactcct gcgacccagg
     4741 cgctatcgac aaaaataatc agcccaatac ccaacgcccc cattagtgca ctggcccgaa
     4801 ccacggcaac gcgactgtaa ctgtcgatga accaaccgcc ggtgaagcgt ccaacggtca
     4861 tccccagggt aaaaccggca taaatcagtg agccggaagt ggggctaaaa ccgtgaccat
     4921 caaccattaa taagggtaac cagtcgttgg cagaaccttc ggcaaaggcc atcgccagca
     4981 ctacaacacc aatcaacagc aactggatat cgcgataaaa aggaatgcct ttttcgccat
     5041 gctgggtgcc atcggcagca tttttgcccg taccgtcagg gattgcctga atggcgatat
     5101 aaataggcgc gatacctacc agcgccgcca ataaaatgtg caccgttgcc ggaacgccaa
     5161 aggccgtcag tgccatcccg acaccagcgc ctgccagcgt gcccaggcta taaaaaccgt
     5221 gcatcatcgg caaaaccgtt ttattcattt ctcgctcaac ggcggcacct tcaacgttta
     5281 tcgccacttc cgcagaacca aaacttgccc caaagacgcc gagaccaacg gcaaagagca
     5341 ggggcgatgt cagccagagt gccagactta atatcatcat cccgatcaat gcgcaggaca
     5401 tcgtgaccag gatgacatta cgtgtcccaa agcgtttcac taaccacgcc gagcagagaa
     5461 taccgctcat cgaaccgatc gacagaccaa agagaacacc gcccatttca gcgatcgaga
     5521 cagagagaat atcgcggata gcaggcgtac gggttgccca ggacgccatt aacaggcctg
     5581 gcaaaaagaa gaacataaac agcgcccagg ttcggcgttt caatgcatta cgtgaagaat
     5641 ttacggtcat agatcacgtc aaaataagaa gagggaagac aacattagca aggttgtgta
     5701 catttgtaca caattgcaga cagaggaaat gacatgcgtc gcgctaacga tccgcaacgg
     5761 cgagaaaaaa ttatccaggc cacgctggag gcggtgaaac tttacggaat acatgctgtt
     5821 acgcaccgca aaattgctac ccttgccggg gtaccgttgg gatcgatgac ctactatttt
     5881 tcaggaattg atgagttgtt gctggaggcg ttcagcagtt ttactgagat tatgtcccgg
     5941 caatatcagg cattttttag cgatgttagt gatgctcaag gcgcatgcca ggctatcacc
     6001 gatatgatct acagctcaca ggttgcaacg ccggataata tggagctgat gtaccagctc
     6061 tacgcgctgg ctagccgaaa accgctatta aaaacggtaa tgcaaaactg gatgcagcgc
     6121 agtcagcaaa cgctcgaaca atggtttgaa cccggaaccg cccgcgcgct tgatgcgttt
     6181 attgagggga tgacgctgca ttttgtcacc gaccgtaagc cgctatcgcg cgaggagatt
     6241 ttgaggatgg ttgagagggt tgcagggtag tagataaatt ttagataaca aaaaacccat
     6301 caaccttgaa ccgaaatggc ggggttgatg ggctccacaa aatggggaca tcaaagaaaa
     6361 gcagtggcac taattaagac tgatgccctg cggaaaagtt ctgcggttgt gcaaaaaaat
     6421 ttcattttca gggcaacttc agttttatcc taatcctggc cataccatga cgatgattgt
     6481 ccctgccagc gtcagcagga cgttggcgat tgcgtaggtg cccgcatagc ccagcgcagg
     6541 gatgttactg cgagctgtat cactgatgat ctccattgcc ggcgcgcagg tgcgtgcgcc
     6601 catcattgcg ccgaacaaca gtgcgcggtt cattcgcaat acataagcac cgaacaagaa
     6661 acagataacc acgggcacaa gactgacaat taatccggca atcaacatct gaccgccaat
     6721 cgcgcccagg ccgttattaa taccgctacc ggcgctcaga ccaacgcctg ccataaacac
     6781 catcaagccg aactctttca ccatgcttaa tgcaccttgc ggaatgtaac cgaaggtcgg
     6841 gtggttagca cgcataaagc ccagcataat tccggcgaat aacaacccgg cagcgttccc
     6901 catgccgaaa ctgaatgtgc tgaactggaa ggtgatcatc ccgatcatca gcccaataac
     6961 aaagaaggcg caaaatgcca gcaggtcagt gacctggctg tgaatcgaga taaagccgat
     7021 gcgatcggcg atggttttta cgcgacgggc atcaccgctg acttgtaaaa cgtcaccttt
     7081 gttaagcacg acgttgtcat ctatcggcat ctcaatctgg ctacgaatga cgcggttaag
     7141 gaagcaaccg tgatcggtca acttcagttg tgcgagacgt ttacctacag cgttatggtt
     7201 tttaacgacc acttcttcag tgacgatacg catgtcgaga aggtcacgat cgaaaacttc
     7261 tttaccgtta cggaagctgg gatcgagtcg ggcatgggcg tcgggatagc ctaccaacgc
     7321 tatttcatcg cccatttgta gcacggcatc accgtctgga tttgccagaa tcccgttacg
     7381 tcgaatacgt tcaatgtagc agccggtttg tcgataaata cccagttcac gcagattttt
     7441 gccgtcggtc caggccacca gctccgggcc gacgcgatag gcgcggatca ccggtaaata
     7501 aaccttacgg ttggcatcag tgtccaggcc acgttcgcgg gcgatttgct gggcgctggt
     7561 ctgtaagtcc tgatgctgca atttcggcaa gtaacgcgca ccaacaatca aactcaccag
     7621 accgattaaa taggttaagg catacccgag gctcagatta tccagtgcca gtgagagctg
     7681 cctgctttcc atgccggaat gatgcagtgt atcgccagca ccgaccagaa ccggtgtcga
     7741 cgtcatagag cctgctaaca taccggccgt caggccaata tcccagccaa acagcttacc
     7801 taaccctaag gcgatcacca gcgcactgcc aaccatcacc agtgctaaca ttaggtaatt
     7861 tttcccatcg cgaaaaaaaa tggaaaaaaa gttcggtccg gcttcgaccc cgacgcagaa
     7921 aataaacagc ataaagccaa gattaagcgc atcggtgtta atgctgaaat gttgttggcc
     7981 taataacagc gatacgacta aaacgccaat ggaattaccc agttggatcg aaccaagtcg
     8041 taactttccg agacatagcc caagcgcgag gaccacaaat aataacagaa tgtaattccc
     8101 atttaacaat tcggcgacgt ttatattcac ggaggctaac ttcttgttta ctagtaagct
     8161 gttgaaagaa atgctaattt acgataatgt gttttaccag aattcagggc gcagattcat
     8221 tcagcgcacc taaacgatag taaagtaaca atatatttta ctagtgtaat cacattaggt
     8281 atcaacggct atatgaattg cgttggccta tattagcatg gaatgcgaag cggctttatc
     8341 ttactgaacg ccacactggc gaaaaatgtg ttcgatagac gcagtgtcag gaggaacgag
     8401 tgaaacataa acaacgttgg gcgggggcaa tctgctgttt tgtcctcttc attgtggtgt
     8461 gcctttttct ggcgacgcac atgaaaggcg cttttcgggc tgccgggcat cctgaaatcg
     8521 gcttgctgtt tttcattctt cctggagcag ttgccagttt cttttcacag cgtagagaag
     8581 tcctgaaacc tctatttggc gcaatgctgg cggcaccctg ttcaatgctc attatgcggc
     8641 tgtttttttc accgacgcgc tcattctggc aagagctggc atggttacta agcgcggtgt
     8701 tctggtgtgc gctgggggca ctgtgtttct tatttatcag tagtttgttt aaaccacagc
     8761 acagaaaaaa tcagtaaagc cctcaacgcg agggcttgtc agacgatcag gcgtccagat
     8821 tttctttcac ccatgcagca aaatcggtat agccgccgat atgttgctga tcgacaaaaa
     8881 tctgcggcac ggtttctacg ggtttacctg ccttttgttg tagatcttct ttagtgatcc
     8941 cttccgcacg aatatctaca tactgatact gaaaatcatc gcgttcattg ctcaatttct
     9001 cagccagatc ttttgcacgc acacagtaag ggcaacccga acgaccaaaa ataacggttt
     9061 gcattatttc tctcctcata gatttatgcc tgtaatgatc acgctaaaat gtattcgctg
     9121 aaagtaggtt taacctgttg cattaattgc taaaagctat aactgttaaa cacaatacag
     9181 tgaaaagttt tagactgaag gctcactttg cagagggaag cgtatgcgcg cgatcggtaa
     9241 attgcctaaa ggcgtgttga tactggaatt tatcggaatg atgctactgg cggtggcgct
     9301 gctgtcggta agcgactccc tgtcgctgcc tgagccattt tctcggccag aagtgcagat
     9361 tctgatgatt tttctcggtg ttttgctcat gcttcccgct gcggtggtgg ttattcttca
     9421 ggtggcaaaa cgtcttgccc cacagctgat gaactgtcca ccgcaatatt cacgttcaga
     9481 aagagaaaaa gataatgacg ccaaccattg aacttatttg tggccatcgc tccattcgcc
     9541 atttcactga tgaacccatt tccgaagcgc agtgtgaggc gattattaac agcgcccgtg
     9601 cgacgtccag ttccagtttt ttgcagtgta gtagcattat tcgcattacc gacaaagcat
     9661 tacgtgaaga actggtgacg ctgaccggcg ggcaaaaaca cgtagcgcaa gcggcggagt
     9721 tctgggtgtt ctgtgccgac tttaaccgcc atttacagat ctgtccggat gctcagctcg
     9781 gcctggcgga acaactgttg ctcggtgtcg ttgatacggc aatgatggcg cagaatgcat
     9841 taaccgcagc ggaatcgctg ggattgggcg gggtatatat cggcggcctg cgcaataata
     9901 ttgaagcggt gacgaaactg ctgaaattac cgcagcatgt tctgccgctg tttgggctgt
     9961 gccttggctg gcctgcggat aatccggatc tcaagccgcg tttaccggcc tccattttgg
    10021 tgcatgaaaa cagctatcaa ccgctggata aagacgcact ggcgcagtat gacgagcaac
    10081 tggcggaata ttacctcacc cgtggcagca ataatcgctg ggatacctgg agcgatcata
    10141 tccgccgaac aatcattaaa gaaagccgcc catttattct ggattatttg cacaaacagg
    10201 gttgggcgac gcgctaaaac cgccacgtcg atgtatgata cgcgggcttt tgaccaggtc
    10261 tgacagagag gtgcagggtg aaaattgcca tattgtcccg ggatggaacg ctctattcgt
    10321 gtaagcggct gcgtgaagcc gctatacagc gcggtcacct ggttgaaatt cttgatccgc
    10381 tttcttgcta catgaacata aatcctgcgg cgtcttctat ttactacaaa ggccgcaagt
    10441 taccccattt tgacgcagtg atcccgcgta ttggcaccgc cattaccttt tatgggacgg
    10501 cggcactgcg ccagttcgag atgctgggga gctatccgct caatgagtcg gtcgccattg
    10561 cccgggcgcg tgacaaattg cgttccatgc aactgctggc gcgtcagggc atcgacctgc
    10621 ctgtcacggg cattgcgcat tcgccggatg ataccagcga tttaatcgac atggtcggtg
    10681 gtgcgccgct ggtggtcaag ttggttgaag gcacgcaggg aattggcgtc gtgctggcgg
    10741 agactcgtca ggcggcggaa agcgtgattg acgctttccg cggtctgaac gcgcatattc
    10801 tggtgcagga atatatcaaa gaggcgcaag ggtgcgatat ccgctgtctg gttgttggtg
    10861 atgaagtggt cgctgcgatt gaacggcggg cgaaagaggg ctattttcgt tccaatttgc
    10921 atcgtggcgg cgcggcaagc gtcgccagta tcacaccaca ggagcgtgaa atcgcgataa
    10981 aagccgcgcg aacgatggcg ctggacgttg ctggtgtgga tattctgcgt gctaatcgcg
    11041 ggccgttggt gatggaagtg aatgcgtcgc cggggctgga aggaatagaa aaaaccaccg
    11101 gtatcgatat cgcgggtaaa atgatccgct ggatcgaacg ccacgctacg acagaatatt
    11161 gcctgaaaac gggtggttag tcgcaatcac attactgatc atggttttgc ctgcgctttt
    11221 tgcgtaagct gtgccggtct ttttatcgaa agaggttgta caaaattatg acatcgctgg
    11281 tcgttcctgg tctggatacg ctgcgtcaat ggctcgatga cctggggatg agtttttttg
    11341 aatgtgataa ctgtcaggct ctgcatctgc cccatatgca gaatttcgac ggtgtctttg
    11401 atgccaaaat cgatctgatc gataacacga tcctgttttc tgccatggcg gaagtccgac
    11461 cttcagccgt attgccgctg gcggcggatt tatctgccat caatgccagt tcgctgaccg
    11521 tgaaagcatt tcttgatatg caggatgata atctgccaaa gctggtggtt tgccagtctt
    11581 tatccgttat gcagggcgta acctatgagc agtttgcatg gttcgtgcgt cagagcgaag
    11641 agcagatttc gatggtcatt cttgaagcta atgcccatca actgctgtta ccgactgacg
    11701 atgaagggca aaacaacgtt accgaaaact attttctcca ctgataattc ctttcgagca
    11761 cgcagtcgct gccgcagtga ctgcgcgctg caaaattatc tgctgttttt aaccttttct
    11821 taaagattat ttcacttctc ttgtgtcgat ttggctttat cacatagagc aaatacgcat
    11881 aaaaatttgt taaataccgt tttttaatcc gagctatagt ctcaaaccct ggctaaagtt
    11941 attcttgcga tgcttctata tagcgagcag tgctggccgg gagagagttc tcttttctta
    12001 caccgcgccg ataaaaaaat atgcacgttt attgcatatc tttcagtgtg acaacttttg
    12061 ttcgtttgtt aacgaacttt cagaaggaaa gagatgtgac cgccttaaat aaaaaatggc
    12121 tatcgggtct ggttgcgggt gctctgatgg ccgtctctgt cggcacgctc gcggctgaac
    12181 aaaaaacact ccacatttat aactggtctg attatatcgc ccctgacacg gtggccaatt
    12241 ttgaaaaaga aaccggtatt aaagtcgtct acgatgtttt cgactctaac gaagtactgg
    12301 aaggcaaatt aatggcgggt agcaccggct ttgacttggt ggttccatct gccagctttc
    12361 tagaacgcca gttgactgcg ggagttttcc agccgctgga caaaagcaaa ttgccggagt
    12421 ggaagaatct cgacccggag ctgctgaagc tggtcgccaa acacgatccc gacaataaat
    12481 ttgctatgcc ctatatgtgg gcgacgaccg ggattggcta taacgttgat aaagttaaag
    12541 ctgtgctggg cgaaaacgcg cccgtcgata gctgggactt gatcctcaaa cctgaaaatc
    12601 tggaaaaact gaaaagctgc ggtgtctctt tcctggatgc gccagaagaa gtttttgcta
    12661 ccgtgttgaa ttatctcggc aaagatccca acagcactaa agcggatgat tacaccggac
    12721 cggcaaccga tctgctgtta aagctgcgcc cgaacattcg ttatttccat tcatctcaat
    12781 acattaacga cctggcaaac ggcgatattt gcgtcgctat cggctgggca ggtgatgtct
    12841 ggcaggcgtc aaaccgcgcg aaggaagcga agaatggagt gaatgtctcg ttctcgattc
    12901 caaaagaagg ggcgatggcg ttctttgatg tattcgccat gccagcggat gccaaaaaca
    12961 aagacgaagc ctatcagttc ctgaattacc tgctgcgccc ggatgtagta gcgcatattt
    13021 ccgaccatgt gttctatgtc aacgccaata aagcagccac gccgctggtg agtgcggaag
    13081 tccgtgagaa cccgggtatt tatccgcctg cggatgttcg tgcgaagctg ttcactctga
    13141 aagtgcagga cccgaaaatc gaccgtgtgc gcacccgcgc gtggaccaaa gtgaagagcg
    13201 gaaaataatc cgcagcctta gatgccgggc gggcgcacca cacccgccgg caattcgcac
    13261 catcatggtg cgcttgcaca cattcaatgc cggagagcag ccgtgaatga cgctatccct
    13321 cgcccgcagg cgaaaacccg taaggcgctg acgccgctat tagaaatccg caacctcacc
    13381 aaatcctacg atggtcaaca tgcggtggat gatgtcagcc tgaccatcta caaaggtgaa
    13441 atcttcgcgc tgctgggcgc atccggctgt ggcaagtcca cgctgctgcg tatgctggca
    13501 ggtttcgaac aaccttctgc cggacagata atgcttgatg gcgtcgattt gtcacaggtt
    13561 ccgccttacc tgcgccccat caatatgatg tttcagtctt acgcgctgtt tccgcatatg
    13621 accgtggaac agaacatcgc ttttggcctg aaacaggaca aactaccgaa agcggaaatt
    13681 tccagtcggg tcaatgagat gctcggcctg gtgcacatgc aggagttcgc caaacgtaaa
    13741 ccgcatcagc tttctggtgg tcagcgacag cgtgtggccc tggcccgaag ccttgccaag
    13801 cgcccgaaac tattactgct cgacgagccg atgggcgcgc tggataaaaa gctgcgtgac
    13861 cggatgcagc ttgaagtggt ggatattctg gagcgcgtcg gtgtgacttg tgtgatggtc
    13921 acccacgatc aggaagaggc gatgaccatg gcggggcgca tcgccattat gaatcgtggg
    13981 aaatttgtcc agattggcga accggaagag atctacgagc atccgactac ccgctatagc
    14041 gccgaattta ttggctcggt aaacgtcttt gaaggcgtac tcaaagagcg tcaggaagat
    14101 ggcttggtgc ttgattcgcc ggggctggtg catccactga aagtcgacgc ggatgcctcg
    14161 gtggtcgata acgtgccggt acatgtggcg ctgcgcccgg aaaaaatcat gctttgcgaa
    14221 gagccgcccg ccaatggttg taacttcgcg gtgggggagg tgatacacat tgcctatctc
    14281 ggcgatcttt cggtgtatca cgttcgtctg aaaagtgggc agatgattag tgcccagcta
    14341 caaaacgccc atcgtcatcg taaagggtta ccgacctggg gcgacgaagt gcgtttgtgc
    14401 tgggaagtgg acagctgtgt ggtgctgacg gtttaaggag caaagatgaa tacacttgaa
    14461 cctgctgccc agtcgaaacc gccgggcgga tttaagctgt ggttgtcgca gctgcaaatg
    14521 aagcatgggc gcaaactggt cattgcgttg ccatatatct ggttgatctt gctgtttctg
    14581 ctgccatttc tgattgtctt taaaataagc ctggcggaga tggcgcgcgc tattccacct
    14641 tataccgaac tgatggagtg ggctgacggg caactgtcca tcactcttaa tctcggtaat
    14701 tttctgcaac tgaccgacga tccgctctat ttcgatgctt atctccagtc gctacaggtg
    14761 gctgcgattt cgacattttg ctgtttactg atcggctatc cgctggcgtg ggcggtggcg
    14821 cacagtaagc cttcgacccg taatatttta ttactactgg tgatcctgcc gtcgtggacc
    14881 tcgtttctga tccgcgttta tgcttggatg ggaatattaa aaaacaacgg tgtgctgaat
    14941 aattttctgc tgtggctggg ggttatcgat caaccgctga ccattcttca taccaatctg
    15001 gcagtttata ttggcattgt ttacgcctac gtgccgttta tggtgctgcc gatttatacc
    15061 gcgttgattc gtattgatta ttcgctggtg gaagcagcgc tggatctcgg tgcacgaccg
    15121 ctgaaaacgt tctttactgt gatcgtgccg ctgactaaag gtgggattat tgccggatcg
    15181 atgctggtgt ttatcccggc tgtgggcgag tttgtgatcc cggaactgct cggtggcccg
    15241 gacagcatca tgatcgggcg cgtgctatgg caagagtttt tcaataaccg cgactggccg
    15301 gtggcctcgg cggtagcgat catcatgttg ctgctgctaa ttgtgccgat aatgtggttc
    15361 cacaaacacc agcaaaaaag cgtgggagaa cacggatgaa taatttaccg gtagttcgtt
    15421 cgccctggcg gattgtgatt ttgctgctgg gctttacctt tctctacgcg ccaatgctga
    15481 tgctggttat ctattcgttt aacagctcga agctggtgac ggtgtggggc ggctggtcaa
    15541 cgcgctggta tggtgagtta ttgcgcgatg acgcgatgat gagtgcggtt ggttcaagcc
    15601 tgacaattgc tgcctgtgcg gcaacggcgg cggcgatcct cgggactatt gcggcggtgg
    15661 tgctggtgcg ctttggcagg tttcgcggat caaatggctt tgcctttatg atcaccgcgc
    15721 cgctagtaat gccagatgtc atcacgggct tgtcgctgtt gttattattc gtcgcgcttg
    15781 ctcatgccat tggctggcct gcggaccgcg gtatgcttac catctggctg gcgcatgtca
    15841 ctttctgtac ggcttatgtg gcggtcgtta tttcgtcgcg tctgcgggaa ctggatcgct
    15901 cgatagaaga agccgcgatg gatctcggtg cgacgccgct gaaagtgttt ttcgtcatta
    15961 cgctaccgat gatcatgccc gcgatcattt ctggctggtt actggctttt actttgtcgc
    16021 ttgatgatct ggtgatcgcc agctttgttt ccgggccggg agccaccacg ttaccgatgc
    16081 tggtcttttc cagcgtgcgg atgggggtga atccggaaat caacgccctg gcaacattga
    16141 tcctcggcgc ggtcggaatt gtcggattta tcgcctggta tctgatggcg cgcgcagaaa
    16201 aacagcggat acgcgacatc caacgtgcaa gacgtggctg aagacactaa aatttgccaa
    16261 cctggctaca taatgccgcg catgtcgcgg cattgttttc atggaagacg aaacgttggg
    16321 attttttaag aaaacatctt catctcatgc tcgcctgaat gtgcctgcgc tggtgcaggt
    16381 ggcggcgctc gccattatta tgatccgtgg cctcgacgtg ctgatgattt tcaatacgct
    16441 gggcgtgcgc ggtattggcg agttcattca tcgcagcgta caaacctgga gtttaacgct
    16501 ggtcttttta agcagtctgg tgctggtttt cattgagatc tggtgtgcgt tttcactggt
    16561 gaaagggcgt cgctgggcgc gctggctata tctgctgaca caaatcaccg ccgcaagtta
    16621 cttgtgggcg gcttcgctgg ggtacggtta tccggagctg ttcagcattc ccggtgaatc
    16681 aaaacgtgaa atcttccata gcctgatgct gcagaagctg ccggatatgc tcatcctgat
    16741 gctgctgttc gttccctcga ccagtcggcg gttcttccag ttgcaataat gtgtataatc
    16801 gtcgcccctg atgatgtgaa ggtcaatgta tgcagtgcgc actttacgac gcgggtcgct
    16861 gtcgttcctg tcagtggata acgcagccga ttccagagca actctccgct aaaaccgccg
    16921 atcttaaaaa tctgctcgcc gattttccgg ttgaggaatg gtgcgcgccg gtgtcaggcc
    16981 cggaacaagg gtttcgtaat aaagccaaaa tggtggtgag tggtagcgtt gaaaaaacac
    17041 tgctcggtat gctgcatcgc gatggcacgc cggaagacct ttgtgactgc ccgctttatc
    17101 cagcctcatt tgcgcccgtt tttgcggcgc taaaaccgtt tatcgcccga gcggggttaa
    17161 cgccctacaa cgtggcgcgt aagcgtggcg aactgaaata cattctgctg actgaaagcc
    17221 agagtgatgg cggcatgatg ctgcgctttg tactgcgttc tgataccaag ctggcgcaac
    17281 tgcgtaaggc gctgccgtgg ttacacgaac aactaccgca gctgaaagtt attaccgtca
    17341 atattcagcc ggtacatatg gcgattatgg aaggggagac ggagatctac ctgaccgaac
    17401 aacaggcact ggcggagcgt tttaatgacg taccgctgtg gatccgtccg caaagtttct
    17461 tccagactaa tccggcggtc gccagccagt tgtacgccac cgcgcgcgac tgggtacgac
    17521 agctgccggt taaacatatg tgggatctgt tctgcggtgt ggggggcttt ggtttacact
    17581 gcgcgacgcc tgacatgcag ttaaccggga tcgaaattgc gccagaggcc attgcctgtg
    17641 caaagcagtc agccgctgaa ctgggcttaa ctcgtttaca atttcaggcg ctggactcca
    17701 ctcagtttgc cactgctcag ggggaggtgc cggagctggt gctggttaac ccgccgcgcc
    17761 gcggcattgg taaaccgctg tgtgattatc tttcaacgat ggcaccgcgt tttattatct
    17821 actccagctg taacgcccaa accatggcga aagatatccg cgaactgcca ggttaccgca
    17881 tcgaacgggt acagcttttt gatatgttcc cgcacaccgc gcactatgaa gtgttgacgc
    17941 tgctggtgaa gcaataaaaa agccacaggt gcggcttcag attgctgatc aggtgcgctt
    18001 tgttcatgcc ggatgcggcg tgaacgcctt atccggccta caaaatcatg ctaaatcaat
    18061 acattgcaga gttgatgtgg gccggataag cgcagcgcat caggcaatct tacttttgtc
    18121 atcagtctca attactgcgg aaaccactgg tcattgattt tctgatatgt accgtcagct
    18181 ttaattgctg ccagcgcgtt attcagtttt tccagcagag ctttgttatc cggacgtaca
    18241 gcgataccca ggccggtgcc gaagtattgc ggatcggtca ctttttcagt cgcaacgccc
    18301 agttgcggat tggttttcag ccattcgttt accaccgcgg tgtcaccaaa taccccatca
    18361 atacgaccat ttttcagatc gataaaggca ttctgataac tgtcatagga gacagttttc
    18421 acttccgggt gctgatcctg aatatatttc tggtgcgtcg taccgttttc catcccaatg
    18481 cgtttgcctt tcaggtcggc aaacgttttg taggtatctt ttttggaaat cacgacggct
    18541 gagttttcat agtagggtgt ggtaaacgaa acctgcttgc tacgctccgg ggtgatatcc
    18601 ataccggaga ttacggcgtc atattttctg aatttcagtg acgggatcag gctgtcgaac
    18661 gcgtgattag taaaagtaca ttctgcctgc atttgtttgc acaaggcttt tgccagatcg
    18721 atatcaaagc cgacaatctc attattagca cctatagatt caaagggtgg ataggtggct
    18781 gaaacgccaa aattgatttt ctctgcggca gaagcaccga aagtaaagga agcaagtaaa
    18841 gcggcaagaa ctaacttttt catgatggaa ctcccgtctg tcaatcttat gatttttggc
    18901 cgtgtctgcg gcatgggata acaatgccat caagtgaatt tatatgcaat aaacatgatt
    18961 aaataattta aatgaaataa aaaaagacgg ataacttagt gagttgtccg tcttcattat
    19021 aagaatttat gcactatgta ggccggataa ggcgtccacg ccgcatccgg cacaggcacc
    19081 gtgctgatgt ctgatgcgac gctggcgcgt cttatcagac ctacaaaccc ccccggcgaa
    19141 tgtacgcagc tacattaatt ccgccgttcg aatgccagtg ctttgcgctc gatcagacgc
    19201 atcatcagcg tcagcaggcc gttgacgacc aggtaaataa tccctgccgc accgaacacc
    19261 attacatcgt aggtgcgtcc gtacaacaac tggctgtatc ccatcacttc catcagcgta
    19321 atggtgtaag ccagagaggt acttttgaac accagcacca cttcgttgga ataagaagag
    19381 agcgagcgtt taaaggcata cggcagcagg atcgccagcg tatctttttt gctcattccc
    19441 agggcgctac aggactgcca ctgaccttct gggatcgcac gaattgcacc gtaaaacagc
    19501 tgcgtggtat acgccgcact gttcagcgat aacgcaatca gcgcacataa ccacggttct
    19561 gacaacaaat gccacagtgc cggatactcc tgcaacgtcg gaaactggcc cggcccgtaa
    19621 taaatcagga agatctgcac cagcagcggc gtaccggtaa acagcgtgat ataaccccgc
    19681 accagccaca ccagcaccgg cgttttcagc gtcaggatga tggtaaaaat caatgccaga
    19741 atcagtgcca caatcagcga ggcaacggtt agcgtcaggc tggtgtgcag ccctttcatc
    19801 agctcgggta aatactcaaa cattagctgg gcctccgctc aaaacgtgtc gcgcgcaggt
    19861 caatgcgttt gagaatgtac tgactgagca gggtgatcac caggtaaatc gccgccgcca
    19921 caatgtacca ggtaaatggt tcctgggtac gagtagcaat gcttttggtt tgtaacatta
    19981 aatcattcac actaatcaaa ctgaccagcg cggtatcttt cagcagcacc agccactggt
    20041 taccgaggcc aggcagcgca tgacgccaca tctgcggcat caccagacgg aaaaagatag
    20101 ccgatttcga cagccccagc gcctgaccag attcccactg gcccaccggt acagctttca
    20161 acgcgccgcg cagcgtttgc gaggcatagg cggcatacag cagtgacaga gcgatgacac
    20221 cacaaaggaa cggactcacg tcgaagttct caatgtccat ctgcaccggg atctgcacga
    20281 acccaagatt gatagtgaag ccatccgaaa gcgtcagcag taactgcgag gagccaaaat
    20341 agataaacag cactaccaga atttctggca ggccacgcag aatggttacc agcgctgaac
    20401 ctgcccacgc gacaggacgc catttcgctg actcccatac cgcaaagaac atcgccagcg
    20461 ccagcccgac aatcaatgca caaacggcaa ggccgacggt catcccggcg gcgcttgcta
    20521 aaggaaaaaa ttcattcatc aggaattact tctggaacca tttgttgtag atggtttcgt
    20581 aagtgccatc tttcttcact ttttccagcg cagtgttgag tttctgctgc agttcagtgt
    20641 ttccctgacg taccgcgatg ccgaggccag taccgaaata atctttatcg gtcactttat
    20701 cgcccactgc cgccagtttt gggttatctt tcagccactc agtgaccact gcggtgtcac
    20761 caaatacgct gtcgatacgc ccgttttgca gatccagttt tgcgttctgg tagctgtcat
    20821 acggaacggt ggtgatttcc gggtgcttat ccataatgaa cttctggtgt gtcgtcccgt
    20881 tctgaacgcc gacttttttg cctttcagct gatcaacact ggtgtatttg ccttgctgac
    20941 ccacaaacag ggcagagttg tcatagtacg gggtagtaaa cagtacctgc ttttcacgct
    21001 ccggagtgat atccatgccc gccatcactg cttctacgcg acggaatttc aggcttggga
    21061 tcaggctgtc aaacgcctgg ttagagaaag tacaggttgc atcaatctct ttacacagtg
    21121 cctgcgccag gtcgacgtca aaaccaacga tctggttgtt tgcatcaatc gattcaaacg
    21181 gaggataggg ggcttcggta gcaaaacgaa tggtttcggc agctgtggcg gaaagactaa
    21241 aacctgcaat taacgcggca atcagaactt ttttcattgt tgttatcccg aatcttagtg
    21301 agagagatag tttttaaatg cttcggtttg cggctcggta aagcagctcg cgtcgccttg
    21361 ttctacgata tgaccatttt ccatatacac cactcggctg gcggttttac gcgccacttc
    21421 aacttcgtgg gtgacgatca cctgggtaat attcgtttct gccagctcac gaatgatgct
    21481 gacgatttgt gccgtaattt ccgggtccag tgcggcggtc ggttcatcga acagcagtac
    21541 ctgcggttcc atcatcaacg cacgggcaat agcaacacgc tgctgctgac caccagaaag
    21601 atgcagcggg taacgatcgc tataaggttt gagacgcaga cgttccagca gtttttctgc
    21661 acgggccagc gcctgatctt tactcaaccc cagtacacgg cagggcgctt caatcaggtt
    21721 ttgctgcacg gtcagatgcg gccacaggtt gtattgctga aacaccatgc caacgttacg
    21781 acgcaaatcg cgaatcgctt tgtcagaggg tgttttggtg aaatcgaaat ggttgcctgc
    21841 aatgttgagc gtaccggagc gcggcatctc aagcagattg agtacacgca gcagcgagct
    21901 tttacccgcg ccgctggggc caagtaacac cagcgtttcg ccctgtgggc aatccagcgt
    21961 gatatcgaac agcgcctgat gcgcgccgta gaagcaatta atgccgttta attgaatact
    22021 cattgacact cgtatactgg cagtctgata gctattgagg tcgaagatag tacctttgac
    22081 agaataatta tgcaatattt atgctttaaa agttaaaagc aaagcgcatt attcaataaa
    22141 catagcacaa aataacgggg gcggtggtcg gtgagcataa atgtcggcat tcctcacgaa
    22201 atgccggata atttacgggg tttattggtt gatcaaggcg ttagcgattc tcgatggact
    22261 gacggagcgt acccgccgtg gcatgaacgc taccgcctaa gtagcgcaca tcgtcgatga
    22321 cccaacactg gccttcctga atcattaaca cttcatcctg ccagccctgg tcaccctgtt
    22381 tgagatccac ccgcaatgga atgttacggg tatcacgatt agggatagtc gatgcactgg
    22441 caacgtgggc gctgtctggc aaggtggttc gactggagaa cggatcgctg cttaacaatt
    22501 cacgatgact attatcgcgg ctggcatcgc taagttgggt tgccagtttg tcactcaaat
    22561 aggggcgcag ggcggtaatg tcattgctgc gatgttgaat gcggtagtcg tagaattgtt
    22621 gagccacact gtcaggccct ccctggacac aggaaccggt gcgggggccg ttatctttat
    22681 aagctggcgt gacggtggta caggcactca gcagcagtgc acatggaata agtagagata
    22741 gtgaacggta gcgcataatc atttccttat tagcttgctg tctgcgtcac actacgacca
    22801 aacgctatca gattatttaa tacattgttt ttaatacaat tctgaaacaa tctgaacacc
    22861 tgattgggtt tgagtccatt gctggcgacc cactcaaggt tgctcgtagc tgacttactt
    22921 aaattaccca aacaatagac agtgtaaacc atttgtgagg ttgagcgtgt tatatggctg
    22981 aaatgatcat ttaaggtaaa tatgatagtt ctgaaatggt ggtataaatc ctcctgtaga
    23041 ggttaagagc atctgcaaga agccagtttc acatttataa cgcgtgataa atgaatgcgg
    23101 ttattgtcta atcattacaa aatttttcac atgcgataag agatcctgcg attaagatga
    23161 ttgccaaaat tacgttgtat ttttgaaaat gagcgaaatt tgatagtccc agctttgact
    23221 ctcgataata ttatttatgc tacaattgtg ggtaaagaat ttaagctaat gaatttgagt
    23281 gaagaataaa ttaaattgct tcagtatttc gttaattaat ataaatacaa aaggcgttgt
    23341 gcttttataa ttataaagcg cgaggagttc ggtggattat cgtttttctt gggtgatttt
    23401 taatatgata aacaatatat ctgatcaagc atcatcattt ccaggcacac aactaaatca
    23461 aagtgataat ttcctggatt cactgaggga atttttcgcg atattaaatc ccagtcgtaa
    23521 gggtgagttg tctacatggg ataccatcta cttgcatctg atattagcaa taaatgctga
    23581 cagtgatctc ataaaaaatg atgtgttgct cgcggaaaat attccatcag cgaattacca
    23641 atttaataca ttttttagca atactttcga gattgatgtt aaaaaatatc tgggtaaatc
    23701 agaagataac gaagttgaaa taaaagcggg taatgaaaga atctctattg gaatacgtaa
    23761 tgtatcgaat ggaaaacttg aaagacaaca gtttcttttc ccactggact atgagaataa
    23821 attacaagaa caattagata agtattttac gattgaatcg cacccattat tatacaggta
    23881 taccattggt agtaagattg cgaatgtgat ttttgaaaag ttatacagtc gtattgactt
    23941 taacaaagaa caatatatat cctttataaa ggacgcattt attcattttt atgattatag
    24001 caggcgatat gctattagtg agaatatcga taaagacgct gtaacaaaca atatcgcatt
    24061 gatgagcact ttttatgaca gtgataatac gtcaggtgag gttttaaata atgattttac
    24121 agaagaagag tcatttgaaa ctgcgttaga tgtagagcat gcaatagtgc ttggatttgc
    24181 tgatgataat tttgaaacaa aacctgttca ttatcaggat ttattaacac gatttagtgc
    24241 ctttcaagat accgtgttta atctgtttcc tgaaatgcac tcttcgcatt accacgatat
    24301 ttgtagcgta agtgtggata tgacaaaggg aacgcaatgc atgattcact tgatggtgaa
    24361 tgaagaagtt ttcatgtctc tgcctgttcc agttgccacg atggtacgtg aagatgcaag
    24421 taatttagtc aatcttaaaa cactactcaa tgatggttgt ttcattaaat atagtcattt
    24481 taatgacgtg gcattaataa agcaaaacat aagtaatctc tatttatcac acactgttgt
    24541 gaatgaaagc atattgaaaa aatgttgttt tgaaaacggc agtttaggtg atgttaaaat
    24601 aacaaatagc aatgtaataa acagtgcatt caaaaatatt tcgtttagat ctgttaaaat
    24661 taataacgta aatactcatt ctttaaaatt tattaactgc aattttttca atgttgatat
    24721 gataagagtt aatctcagca aatgtctttt tcatgaatgt agtatgcatg gtgtcaagat
    24781 aaaaccctgg ttgcctgtaa aatggacaaa agaattaata agtgattatt tgtatggctg
    24841 tttactatct ctgtactcta tctgtgcaag ggatatatat aatatgaacg ccgggaataa
    24901 cgtgaaggtt gctgcggatg ctttccttga aattattttc tctcttaaaa ataaatattg
    24961 tattaaacta ttaagcgctc aggatcgggc gtttatatac gagtttgcaa gaatgatctt
    25021 cgcttatatt aatgacaaaa gcattgagat cttattactt tcttgttttg cggcggctga
    25081 ccaaaaagca atccagcggt atagacctca gtcacaagat ggggaagact ttaggagtca
    25141 tttacaatat aagttgcctc tttccgctca ttaatgttgt tggtgcaaca taataagaca
    25201 ggtaagcggt accctataat gaaaccaata tcgggttatg caacggataa tcgtaaagac
    25261 ttaagtgggt gtttttgaca caatgagatg atttcctaaa ctgatcaaaa ccttcttccc
    25321 aacatggcat atcagtccga taatgcttta gagatcgaag gcttaacaaa caaggagatc
    25381 gatcatgcaa ttttctacaa ccccaactct ggaaggcctg accatcgttg aatattgcgg
    25441 tgtggtgacc ggcgaagcga ttttaggtgc caatattttc cgtgatttct ttgccggtat
    25501 ccgcgatatc gttggcggac gttccggtgc gtatgaaaaa gaactgcgta aagcacggga
    25561 gatcgccttt gaggaattag gctcccaggc gcgggcgctg ggggccgatg ccgtagttgg
    25621 tattgatatc gactacgaaa cggtcgggca aaacggcagt atgctgatgg ttagcgtcag
    25681 cggtacggcg gtgaaaacgc gtcgatgaga agagtcttct ggctggtcgc tgccgctctg
    25741 ttactggcag ggtgtgcagg cgaaaaaggc attgtcgaga aagagggata tcagcttgat
    25801 acccgacacc aggcgcaggc ggcgtatccg cgcattaaag tgctggtgat ccactacacc
    25861 gcagatgatt ttgatagctc gctggcgaca ctgactgata agcaggtcag ctcgcattat
    25921 ctgatccctg cggtgccacc gcgatacaac ggtaaaccgc gcatctggca actggtgccg
    25981 gaacaagaac tggcctggca tgcggggatt agcgcctggc gcggggcaac gcgccttaac
    26041 gacacctcta ttggcattga gctggaaaac cgtggctggc aaaaatcatc cggagtgaaa
    26101 tattttgccc cgtttgaaca ggcacagatt caggcgctta ttccgctggc gaaagatatt
    26161 attgcccgtt atcacatcaa gccggaaaac gtagtggcac atgcggatat cgcaccgcag
    26221 cgcaaagacg atccggggcc attatttccc tggcagcaac tggcgcagca ggggattggt
    26281 gcctggccgg atgcgcagcg ggtgaacttt taccttgccg ggcgcgcgcc gcacacaccg
    26341 gtagataccg cgtcattgct ggagcttttg gcgcgctacg gttatgacgt aaaacctgat
    26401 atgacaccgc gcgagcagcg gcgcgtgatt atggcattcc agatgcattt ccgcccgacg
    26461 ttatataacg gcgaagcgga tgcagaaact caggcgattg ccgaagcatt gctggagaaa
    26521 tacgggcagg attagcgcgg cagttttccg tggtcgcgta gccaggcggc agttttctcg
    26581 atgccttcat ccagggtgat gaccggctga taacctaact cttcctgcgc acgcgtaata
    26641 tccagcgtaa agtcaaaatt caacttggag gcgccgtagt gggtcagcgg cggctctttt
    26701 gctgacttgc ggcctaaacg ctccatgctg cgggcgatca tatccagcat cgggtaaggg
    26761 acggaacgaa tacgacagtc aatattcaac tcgtcgatca gcttctgcac gatgctgcgc
    26821 agtgtgcgat gctcgccgtt ggtgatgttg tacacacgcc cggaaggtag cttatcgcag
    26881 gcttcctggc ttgccagcca cattgcgtgc acggcatttt catagtaggt catatcgacc
    26941 agcgcactgc cgccatgcgg taacagaata ctgccgtagt ggtgcatcat atgcgccaga
    27001 cggggaataa agactttatc gtgcggtccg aacagacttt gtgggcgcag aatagtaaag
    27061 cgcgtttgtg gattcgcctg cgaaagcata ttgatcactt cttcgctggc tgctttgctg
    27121 cgggcaaact cgttggcgaa gcggtgaggg cgaaaatctt ctttaatatt gcgatggtgg
    27181 tgataatcga agtacaggga gggggaagag atatgaataa agttacgcac accccaggcg
    27241 acagcccatt cacccaggcg gcgagtggcg cgaacgttag ccagatcgaa agcctgttgt
    27301 gtcccccagg gtgaggtaaa gctggagcag tgccacagcg tatcaatgcc cgcgagcatc
    27361 actttagctt gtgatgaaac cagctcggtc agatccgtcg gaacaaactc tgcgcccatt
    27421 ttttccagca atttacccat tgcttcgttg cgaccgctcg cacgcacgct gatgcctttc
    27481 tggcataaaa actctaccgc gtttcgacct aagccgctgg tggcgccggt aaccagtacc
    27541 ttcatatcaa tccactgttg ttgagaaaat gacgtgcgca ttcttccgtg atttccccca
    27601 tgatgcaatg ggaaacatga aagaataacg caggttttgt cgattaatct gtgctttgtt
    27661 ctgccagtct ggcgatttgt tttgccattc cgcgaaaaat aaacagatgc gcggggatca
    27721 tcaataacca gtaaaacagc cccggcatac cgtgcggatg ccagaaagcg cggacatcga
    27781 tagtacgata gtcaccttta tcttccaggg taaaacacag tcgtcccagc cccggcgctt
    27841 tcatgccaaa taacaacgta agttgctttt tcggttcaac gacaatcact ttccagctat
    27901 ccaccgcatc gccggtctgt aaatattcgc gctccgggcg gcctttcgcc agattatgac
    27961 cgatcgcgcg gtccatcaac gcccgtgtct gccacaaaat attgccaaag aaataacgct
    28021 ctttaccgcc gatttggttc accaccagcc ataaagccgc aaggctggcg gacgttttaa
    28081 cggtaaaccc cgcctgtttg gcgaaataac cgtactccgg tcgccagcgg gcaaaggcct
    28141 gagcgtcgta gccccagtcg ctggagttga ccagtttttc ttcctctttc aacgtgctac
    28201 gtaccgcgtc atcgaaagcg atcagccgtt gtgggatgag tgcacgtagc gcggtatcat
    28261 ctgccagcag atcgtgtttc agcccctgaa tcaacgccct ggcggtggtg ggcggtacgg
    28321 aagtaatcac attgagaaac cacaccgaaa tccagcgggt ggggaggggg atggggatca
    28381 accagcggcg cttaccgctc accgccataa aatgttcaaa ctgttgctga taactgagca
    28441 cctctggtcc ggcggcttcg aagatgcggt gttcgttggc tggatggtct aacagcgcca
    28501 ccagatagtg cagcaagttt tccagcgcga tgggcgtggt gcgtgaacgt acccagcgtg
    28561 gcggcgttaa cactggcagg ttgtagacca tatcgcgcat gacttcgaac gccgctgaac
    28621 ctgcgccaac gataattccg gcccgaagtt cggtcactgg tacattcgct tcacgaagaa
    28681 tgtccgccgt agcctgacga gctcgcagat gatccgactg ctcatgtggc ggggcctgca
    28741 acgaactgag aaagattaat tgcttaactg gtacttcacg tagcgcatcg cggacgttga
    28801 gagccagctg gcgctcctga gcgataaaat cgccgccttc gcccatgctg tgcaccagaa
    28861 aatagacggt atcgatatcc tgcaacaggg ccggaaggtt atccggccag ctgagatcga
    28921 ctttatggca actgacgttt gccagttgca gctttgcaag cctgtcgaca tgacgtgccg
    28981 ccgctaggat ctgatgccct tgctggctga gtgtgcgcac cagatgctga ccaatgtagc
    29041 cactggcacc gagaactaaa atgcgttgcg gcacgtctct ctccttaacg cgccaggaat
    29101 gcgcgccagt gggcggcgac ttccgccagt tgttcgcgcg agacgtcaag atgcgtcacc
    29161 aggcggacaa tcggcgaggc gttaatcagc acgtttctcg ctttcatgta ttcgcctaac
    29221 gcggcagcat tttcttcccc gacgcgaaca aacagcatat tagtgtcctg acgcatcaca
    29281 tccgcgcctg cttcacgcag ttgctccgcc atccaggcgg cgttgtcgtg atcttcctgc
    29341 aaccgtgcga cgttattttc cagggcatac atcccggcgg cagccagaat cccggactga
    29401 cgcatcccgc caccggccat tttccgccag cggatggcac gtttaatgta atcacgatta
    29461 ccgacgagta atgaaccgac tggcgtccca agacctttcg acaggcaaat ggtgaacgaa
    29521 tcacaatatt gcgtgatctc tttcagttcg cagccgtaag ccaccacggc attaaagatg
    29581 cgcgcaccgt caacatgcag cgccagattg cgctcgcggg taaattccca tgcttctttc
    29641 aggtattcac gcggcagcac tttgccgttg tgggtgtttt ccagactgag taatttggtg
    29701 cgggcgaaat ggatatcgtc gggtttgatt ttcatcgcca ctttatccag cggtagcgtg
    29761 ccgtcggcag ccgcgtcgat gggttgcggc tgaatactgc ccagcaccgc tgcgccaccg
    29821 gcttcaaaca gatagttatg cgcggcctga ccgacaatat actcttcgcc acgttcgcag
    29881 tgactgagca gagcgaccag gttggcctga gtgccggtag gcagaaaaat ggcggcttct
    29941 ttaccggaaa gctctgctgc atagtcctgc agagcattaa cggtagggtc gtctccgtaa
    30001 acgtcgtccc caaccggggc ggccatcatc gcttcgagca tggcgcggct cggtcgggta
    30061 acggtatcac tgcgtaaatc aatcatggca tgtccttatt atgacgggaa atgccaccct
    30121 ttttacctta gccagttcgt tttcgccagt tcgatcactt catcgccgcg tccgctgata
    30181 attgcgcgca acatatacag gctaaaacct ttggcctgtt cgagtttgat ctgcggtgga
    30241 atggctaatt cttctttggc gaccaccaca tccaccaaca ccggaccgtc gatagagaag
    30301 gcgcgttgca gggcttcatc aacttcagag gctttttcta cacggatacc cgtaatgccg
    30361 cacgcttcgg caatgcgggc aaagttggtg tcgtgcagct cagtaccgtc tgtcaggtaa
    30421 cctccggcct tcatctccat cgccacaaag cccagcacgc tgttattaaa gacgataatt
    30481 ttcaccggca gtttcatctg cactactgag aggaaatcgc ccatcaacat gctaaaaccg
    30541 ccatcgccgc acatggcgac cacctgacgt tccggctcgg ttgcctgcgc acccaacgcc
    30601 tgcggcatgg cgttagccat cgaaccgtgg ttaaacgaac ctaacaggcg acgcttgccg
    30661 ttcattttca gataacgtgc cgcccatacc gttggcgtgc cgacgtcaca ggtaaagatg
    30721 gcgtcatcgg cggcaaaatg actaatttgc tgcgccagat attgcgggtg aatggctttc
    30781 tcgctcggtt tagctaaatc gtccagccct ttgcgggcat cgcggtaatc ttccagcgct
    30841 ttatccagaa acttgcgatc ggttttttct tccaccagtg gcagcaatgc gcgcagggtt
    30901 gatttgatat cgccgaccag cgccatatct accttgctat gcgcgccgat gctggctgga
    30961 ttaatatcaa tctgaataat tttggcatcg gtcgggtaga acgcacggta ggggaactga
    31021 gtaccgagta gcactaacgt gtcggcgttc atcatggtat ggaaacctga cgagaatccg
    31081 attaacccgg tcattccaac atcatacgga ttatcgtatt cgacatgctc tttaccgcgt
    31141 agggcatgaa caataggtgc tttaattttc ccggcaaact caactaactc tttatgcgcc
    31201 cccgcgcagc cgctgccaca catcagggcg atattgctgg aataacgcag cagttgcgcc
    31261 agtttgcgta actcttcttc ttccggcatc acgactggct gtggcgcatg ataccagtgc
    31321 gtggttgccc cttctggcgc aggttttaac gccacgtcgc ccggtaatac aacaacggaa
    31381 acgccacggt taagcaccgc tttgcgcata gcaattgcca gcacttgtgg gatctgctcc
    31441 gggctggaaa ccagctcgca atagtgacta cattcgcgga atagctcttg tgggtgggtt
    31501 tcctggaaat agccgctgcc aatttcgctg gagggaatat gagcggcaat cgccagtacc
    31561 ggaacgttat tgcggtggca atcgaatagg ccgttgatca agtgcaggtt gcctgggccg
    31621 cacgatccgg cacagaccgc cagttctcct gaaagttgtg cttcagcgcc agcggcaaac
    31681 gccgccactt cttcatggcg ggtggacatc cactcgatgg tgcccatgcg attaagactg
    31741 tcgctaagac cgttcagaga gtcgcccgtg actccccaga tgcgtttcac gcctgccgat
    31801 tcgagtgttt tggcgatata agctgcaacc gtttgtttca tggttctcca tctcctgaat
    31861 gtgataacgg taacaagttt agttcatctg acggaggggg aagggatggg agagaaagga
    31921 ggcactaacg gttaaatagc ccgatgaaag gaatatcatc gggcataagg cgattatgcg
    31981 agaaccaaat ccccctgcgg atggcaggag caggccagta cgtaaccttc agcgatttcg
    32041 gcgtcggtca gcgtcattgt actgctcacc gtatattcac cggaaaccac ttttgtctta
    32101 cagcagccgc aaacacccgc acggcaggca gcgacaaccg gaacgttatt gctttccagc
    32161 gcctccagta gcgtggtgcc aaccggggcg taaaattctc gtgccggttg cagtttggtg
    32221 aatttcagac cgctggtcgc cgcttctgct actggggtga agaatttctc tttaaagaaa
    32281 cgcgtcacgc cgagcgcttt cacttcctgc tctacccaat ccatatacgg agccgggccg
    32341 caggtcatca cggtacgtga agctaagtca ggtacatctg ccagcagttc gcgagtgagg
    32401 cgaccagcga taaagccttc ggtaacgtta ttttctgcca ccagcgttac cggatagtta
    32461 cgccactcat cggcgaaaat aacatcctgc ggcgtacgca cgttgtagat cacccgcaca
    32521 tcggcctgtg gacggttctt cgcaagccag cgacgcatcg acataatcgg cgtgacgccg
    32581 cagcctgccg ccagcaacag gaatttatct tctgctttat cgtcgcaggt aaattccccc
    32641 atcgcgtccg aaagccagag ataatcaccg cgttttacat cgcgcgtcag ccactgggag
    32701 ccgacaccgt catcaatccg ccgcacggtc agggtgatat attcactcac gcctggcgtg
    32761 gaggaaatgg tgtaagcacg cagcgtttcc gctgagtttc gcacgctgac cagtgcatat
    32821 tgcccggcgc gatatgggta gtgatcgtgg caaatcaggg aaatcgtcca cacatccggc
    32881 gtttcttgcg taatgtgatg aacctgtatc cgccacgggc attgattcgt tggcatcgtc
    32941 atcgacaaac tccttacgcg ttcaacagtt gcttcatgtc ttcttcaaca gtggtgatag
    33001 aacgcaggcc gaatttctcg ttcagcaccg ccagcaggtc tggtgtcagg aaaccaggtg
    33061 cagtcgggcc ggtgacgata tttttcacgc ccagagaaag cagcgtcagc agaatgacaa
    33121 tcgctttctg ttcaaaccag gagagcacca gcgacagcgg cagatcgttc acaccacagc
    33181 ccagtttctc tgccagagtg acagccagaa taattgctga gtaagcatcg ttacattgac
    33241 ctgcatctac cagacgcggc agaccttcga tatcgccaaa ctcaagtttg ttaaagcgat
    33301 atttaccaca ggcgagggtc aggatcaggc agtcatccgg cacgctggtg gcgaaatcgg
    33361 tgaagtagtg gcgctcgccg cgtgcgccgt cacagccacc aagcaggaag atatgacgca
    33421 gtttttcacg gctaaccaga tcaatcagcg tatcggctgc accaagcagc gtctggcgac
    33481 caaagcccac ggtgatcagg tgaggaattt cgctgtacgg gaagcccgcc atttgttgcg
    33541 cctgggcgat gaccgcagag aaatcttcgc catccagatg acgcacgcca ggccagccaa
    33601 cgatgctgcg ggtccagata cgatcgtcat aagcgcctac ggttgggtcg atgatgcagt
    33661 tcgaggtcat cacgatgggg ccagggaaac gagcgaactc cacttgctga ttctgccagc
    33721 cgctgccgta gttaccgacc agatgcttga atttacgcag ctccgggtag ccatgcgcag
    33781 gcagcatttc gccgtgggtg tagacattaa cgcccgtgcc ttcggtctgt tccagcaggt
    33841 tgtagagatc tttgagatcg tgaccggaaa tcagaatgca tttacccgcc gtcgctttga
    33901 cgttgacctg ggttggcgtc gggtgaccgt atttaccggt ttcgcctgca tccagaatgc
    33961 tcatcacttt gaagttcatc tggccgattt ccattgaaca ctcaagaagc gcgttcatat
    34021 cggcaggcca ggtccccagc cacgccatga ttttatggta ctgggcataa atatcgttgt
    34081 cgtattgacc gagaacatgc gcgtgttcca tataggccgc cgcacctttc aggccataca
    34141 ggcacagcag acgcaggccg agaatgtttt cgccaatcgc cgctttatct ttgttagggg
    34201 taaattctgc tgcctgacgt tgcagctcgc cgagatcatc gctcaccagt tgcaggtcag
    34261 ccatcgggtt atcgacgcgc gcgttggcat ctacagccag gcattgtgct ttcagcgcct
    34321 cgcgcaaggc aatcgcttca cgagcgtagc cgacaatacg cggagaatcg aagttaacgt
    34381 tggtcagggt tgagaaaaag gcacgtggcg cgaagctgtc aacatcgtgg ttgatgatgc
    34441 cgtattcacg cgcttttacc gcccaggcag aaagcccttg cagcgccgcg atgagtaaat
    34501 cctgaaggtc agaagtttcc gccgttttac cacacatccc ctgcgcgtat gagcagccgt
    34561 tttctgccgg agtacggata gtttgttcac attgcacaca aaacatgatc acacctttta
    34621 aagttatatt taatatacat gtttaagggt aagacgctta acgcggggat aaaagggatt
    34681 tttcatgcaa ctttaaggga gattgattta gcgcaatttt ggcggcaggg aactaccgcc
    34741 agagaggtat tacgcagaga aaaaggcgat gaggatcggc actaacaggc taagaataaa
    34801 accgtgaaca attgccgccg ggaccatatc cagcccgcca gtacgttgaa gaacgggcag
    34861 ggtgaaatcc attgatgtgg caccgcataa gcccagtgca gtagagcggc tgcggcgaat
    34921 cagcccaggg atcaacataa tagcaatcag ttcacgggcc agatcattaa aaaacgccgc
    34981 gctcccgatt accggaccaa aagattcggt caataaaata ccggaaagag aataccagcc
    35041 gaaaccggag gccattgcca gcgcggtatt gatggggaga tcaagaataa aggcgttaat
    35101 taaaccacca attaatgaac tgacaaccac caccacggcg acaatcattc cccggcgatt
    35161 aaggacaatc tgctttaagg tcatgccatt attgcgcaac tgaataccaa cgaggaaaag
    35221 tagcaaaatt aacgtgtatt cactggcttc ggtcgcgtgt tgtaagaaag ccagtccact
    35281 cagaccaatg gcaaaaccaa tcactactac gccgcacagt tttagcgact ccagcgccat
    35341 cgcaatacgc gacgggagtt tttcttgctg atggtggttg cgccacggca ggcctcgctc
    35401 cagccacatc agggcggcaa tattacacag taaaataacg gtaatactga cggcagaata
    35461 atgcagaatc gccaacaggt tactggcgag gttatcgaga aacgccagac tgatacccat
    35521 aaaaaagaga ataaggtaaa ccatccagct taatagctga ttaatagctt ttaacgcagc
    35581 ttgttggcga agcggaatga ggtaacccac aatcagggga accagaatga ttaacagccc
    35641 agaaaacatg aaaatccagt ccttgcaaag aagaagtcga aatgcgcgat gacacactac
    35701 tgaaagcgga agggcgagta aagttgcaat taaaaggaaa tgttatgcat cagag
//
LOCUS       DAEBXN010000032        34996 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.032, whole genome shotgun sequence.
ACCESSION   DAEBXN010000032 DAEBXN010000000
VERSION     DAEBXN010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34996
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.032"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(<1..815)
                     /locus_tag="KJL99_001643"
     CDS             complement(<1..815)
                     /locus_tag="KJL99_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HBD4436405.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLTSS"
     gene            907..993
                     /locus_tag="KJL99_001644"
                     /pseudo
     CDS             907..993
                     /locus_tag="KJL99_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1398..1874)
                     /locus_tag="KJL99_001645"
     CDS             complement(1398..1874)
                     /locus_tag="KJL99_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HBD4436406.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(1933..3222)
                     /gene="bioA"
                     /locus_tag="KJL99_001646"
     CDS             complement(1933..3222)
                     /gene="bioA"
                     /locus_tag="KJL99_001646"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HBD4436407.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            3309..4349
                     /gene="bioB"
                     /locus_tag="KJL99_001647"
     CDS             3309..4349
                     /gene="bioB"
                     /locus_tag="KJL99_001647"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HBD4436408.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFEPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLGQALMT
                     PDTDEYYNAAAL"
     gene            4346..5500
                     /gene="bioF"
                     /locus_tag="KJL99_001648"
     CDS             4346..5500
                     /gene="bioF"
                     /locus_tag="KJL99_001648"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HBD4436409.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            5487..6242
                     /gene="bioC"
                     /locus_tag="KJL99_001649"
     CDS             5487..6242
                     /gene="bioC"
                     /locus_tag="KJL99_001649"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HBD4436410.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQARQAVDERP
                     HANRFLPPDEIEESLNGVRYQHHIQLITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            6235..6912
                     /gene="bioD"
                     /locus_tag="KJL99_001650"
     CDS             6235..6912
                     /gene="bioD"
                     /locus_tag="KJL99_001650"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HBD4436411.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAESTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            7491..9512
                     /gene="uvrB"
                     /locus_tag="KJL99_001651"
     CDS             7491..9512
                     /gene="uvrB"
                     /locus_tag="KJL99_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HBD4436412.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVIVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMDELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDRSTPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMLPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(9704..10612)
                     /gene="yvcK"
                     /locus_tag="KJL99_001652"
     CDS             complement(9704..10612)
                     /gene="yvcK"
                     /locus_tag="KJL99_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HBD4436413.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPNVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      10877..11021
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            11009..11998
                     /gene="moaA"
                     /locus_tag="KJL99_001653"
     CDS             11009..11998
                     /gene="moaA"
                     /locus_tag="KJL99_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HBD4436414.1"
                     /translation="MASQLTDVFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEGDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            12020..12532
                     /gene="moaB"
                     /locus_tag="KJL99_001654"
     CDS             12020..12532
                     /gene="moaB"
                     /locus_tag="KJL99_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HBD4436415.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDEAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            12535..13020
                     /gene="moaC"
                     /locus_tag="KJL99_001655"
     CDS             12535..13020
                     /gene="moaC"
                     /locus_tag="KJL99_001655"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HBD4436416.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            13013..13258
                     /gene="moaD"
                     /locus_tag="KJL99_001656"
     CDS             13013..13258
                     /gene="moaD"
                     /locus_tag="KJL99_001656"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HBD4436417.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            13260..13712
                     /gene="moaE"
                     /locus_tag="KJL99_001657"
     CDS             13260..13712
                     /gene="moaE"
                     /locus_tag="KJL99_001657"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HBD4436418.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDLQAAKRW"
     gene            13849..14553
                     /locus_tag="KJL99_001658"
     CDS             13849..14553
                     /locus_tag="KJL99_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HBD4436419.1"
                     /translation="MDRFPRSDSIVQPWAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            14758..15471
                     /locus_tag="KJL99_001659"
     CDS             14758..15471
                     /locus_tag="KJL99_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HBD4436420.1"
                     /translation="MESYSQNSNKLDFQHEARILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPPMYVAIGLLLLCMYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLVP
                     VYAIIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERP
                     IWVISCLMIVLWPGIISHGRNKLLELAGKCHSEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(15507..16463)
                     /locus_tag="KJL99_001660"
     CDS             complement(15507..16463)
                     /locus_tag="KJL99_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HBD4436421.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRISSLSITTNWLGYILLAGIIFTAGVVELPDYWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(16463..17704)
                     /gene="clsB"
                     /locus_tag="KJL99_001661"
     CDS             complement(16463..17704)
                     /gene="clsB"
                     /locus_tag="KJL99_001661"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HBD4436422.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPRQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     AQREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVNP"
     gene            complement(17701..18462)
                     /locus_tag="KJL99_001662"
     CDS             complement(17701..18462)
                     /locus_tag="KJL99_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBD4436423.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            18595..19005
                     /locus_tag="KJL99_001663"
     CDS             18595..19005
                     /locus_tag="KJL99_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HBD4436424.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(18967..20073)
                     /locus_tag="KJL99_001664"
     CDS             complement(18967..20073)
                     /locus_tag="KJL99_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBD4436425.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(20084..21217)
                     /locus_tag="KJL99_001665"
     CDS             complement(20084..21217)
                     /locus_tag="KJL99_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBD4436426.1"
                     /translation="MSNPILSWHRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQSGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(21210..22946)
                     /locus_tag="KJL99_001666"
     CDS             complement(21210..22946)
                     /locus_tag="KJL99_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBD4436427.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(22939..23934)
                     /gene="hlyD"
                     /locus_tag="KJL99_001667"
     CDS             complement(22939..23934)
                     /gene="hlyD"
                     /locus_tag="KJL99_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HBD4436428.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(23937..24608)
                     /gene="cecR"
                     /locus_tag="KJL99_001668"
     CDS             complement(23937..24608)
                     /gene="cecR"
                     /locus_tag="KJL99_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HBD4436429.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            24837..26024
                     /gene="rhlE"
                     /locus_tag="KJL99_001669"
                     /pseudo
     CDS             24837..26024
                     /gene="rhlE"
                     /locus_tag="KJL99_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308902.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
     gene            complement(26231..26713)
                     /gene="ybiA"
                     /locus_tag="KJL99_001670"
     CDS             complement(26231..26713)
                     /gene="ybiA"
                     /locus_tag="KJL99_001670"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HBD4436430.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAARPIKVDGNTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            26833..28983
                     /gene="dinG"
                     /locus_tag="KJL99_001671"
     CDS             26833..28983
                     /gene="dinG"
                     /locus_tag="KJL99_001671"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HBD4436431.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVTRREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITVPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDELLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEVQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKYLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            29011..29973
                     /gene="ybiB"
                     /locus_tag="KJL99_001672"
     CDS             29011..29973
                     /gene="ybiB"
                     /locus_tag="KJL99_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HBD4436432.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALCLSSVSHPEYIGRVAKFFSDI
                     GGWALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WTERCLAGSEAIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            30114..31199
                     /gene="hcxB"
                     /locus_tag="KJL99_001673"
     CDS             30114..31199
                     /gene="hcxB"
                     /locus_tag="KJL99_001673"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HBD4436433.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCSAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            complement(31428..31688)
                     /locus_tag="KJL99_001674"
     CDS             complement(31428..31688)
                     /locus_tag="KJL99_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBD4436434.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(31953..32219)
                     /gene="ybiI"
                     /locus_tag="KJL99_001675"
     CDS             complement(31953..32219)
                     /gene="ybiI"
                     /locus_tag="KJL99_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HBD4436435.1"
                     /translation="MASAWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPSVRLCIHCQQEKDLQKPAYTGYNRRGSKDRQLR"
     gene            complement(32293..32970)
                     /gene="ybiX"
                     /locus_tag="KJL99_001676"
     CDS             complement(32293..32970)
                     /gene="ybiX"
                     /locus_tag="KJL99_001676"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HBD4436436.1"
                     /translation="MMYHIPGVLSPQDVAHFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(33012..34996)
                     /locus_tag="KJL99_001677"
                     /pseudo
     CDS             complement(33012..34996)
                     /locus_tag="KJL99_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         8293 a         8969 c         9455 g         8279 t
ORIGIN      
        1 gaagaggtta atctttgcag ggcttgcaga gtgcgagtcg acaatggatt gttataaata
       61 ttaacatctg cataaatgtg cagattcatg atactgtctg gcaatgtgtt cagctgattg
      121 ttagggatat atagtgattg cagacgttgt ggaagttcgg gcagtctgtg tagttggttt
      181 tccccaacgt ttaaaagcct cagattttga ggaaggggcg gtagctcttg taatctgtta
      241 tcaaaagcac ttagttcctg tagttcacat ggtaattctg gtaaggcagt aagctggttg
      301 ttgccaacag aaagtttttg tagagccggg ggaagatcag gtagcgtctc caggctattg
      361 agaagggctg agagtgactg caaggaagat ggaagagaag gcaaacatgt taaaactctg
      421 ttaagtgaaa catcaagagc gaccaggtga ggcggaagag ccgggagcct gctcagtctg
      481 ttatcgctgg ctttaagtac agttaaggac ggagggagtt ctggcagaga gcgtagctca
      541 ttgctagaga tgttaagttc ttgtatgtgc gggggcaggt atgggagaga gcgtaatctg
      601 agtaaactta aattgagggc tggctcttga aaagccagac atattttcag taatcgaact
      661 gcctgtgttc ggtcttctgt tgcagcacct tccttggccc agttatccca gatgcggtga
      721 taatccgtga tattttgctc ttctacggat aaacgggata tacatcctaa accacaaaag
      781 gagcggaaaa aagaaggatt gtgggcaggt ttcatttatt attgatcctc attgtttata
      841 tattaatata ttcgtgcaaa tggagtgagg attctttaaa aaaacgcgcc tgtaaatagt
      901 taagaacagg aagaactgtt ggggatgtgg ctggccgaaa atgaaggggt gaagttctgg
      961 ctcaatgtgc tgactgaact gaaaaaacgc ggttggtcgc cctacctttt catcaagcca
     1021 gtagtctgcc caccacagca tcatttctct gcgcttatcg agatactgag catggttgta
     1081 aattcagcta acccagtgcg ttgacaaaaa attagcgcaa gaggacaaaa aatcaccttg
     1141 cgctaatgct ctgtatcagg tcactaatac catctaagtg gttgattcat agtgactgga
     1201 tatgttgtgt tttgcagtat tatgcagtct attttttaag ctaaatgtat tataatgcat
     1261 tgatattaat gatttttact gtttctcggt cagctttttt atactaactt gagcgaaacg
     1321 ggaaggtaaa aagacaaaaa gttgttttta atacctttaa gtgataccag atggcattgc
     1381 gccatctggc agagtgatta actaaacatc gcggtaatcg atgcgcttgc cagagagtgg
     1441 aaatgaacgt taaacccgac catcgcgccg ctggctcctt catcgacatc aatacgctct
     1501 acatccagcg cgtgaacggt aaaaatgtag cgatgggttt cgcctttcgg cggcgctgcg
     1561 ccatcgtatc cggttttacc aaaatcggta cgcgtctgca aaacgccgtc tggcattgct
     1621 accagaccag agccaaaccc ttgcggtaat acgcgggtat cagcgggtaa attaacaact
     1681 acccagtgcc accagccgga gccggttggc gcatccgggt cgtagcaggt gacaacaaaa
     1741 cttttcgttc ccgcaggaac atcatcccac gccagatgcg gtgaaatatt atcgccatcg
     1801 taacccatgc cgttaaagac atgacgatgc ggcaacttat cgccatcgcg cagatcgtta
     1861 ctgatgagtt tcatgaaccc tcctttcttg tttgcagaaa gtgtagccag aaaccctcac
     1921 gctgacttcc cgttattggc aaaaaaatgt ttcatcctgt accgcgcggt taaccgctgc
     1981 ggtcagacgc tgcaactgtt gcgggagaat aatatagggc ggcatcaggt aaatcagttt
     2041 gccaaaaggc cggatccata ccccctgttc gacaaagaat ttttgcagcg ccgccatatt
     2101 caccggacga gtggtttcga ccacaccgat tgcccccagt acgcgcacat cggcaaccat
     2161 ttcggcatca cgggctggtg ccagttgctc gcgcagctgc acttcaatag ccgccacctg
     2221 ctgctgccat tcgccggatt cgataatcgc taggctggcg tttgctgccg cgcaggccag
     2281 cggattgccc ataaaagttg gcccatgcat aaagcagccg gcttcgccgt tactgatggt
     2341 ttctgcaacc tcgcgcgtgg taagtgtggc ggaaagggtc attgtgccgc cggttaaggc
     2401 tttaccgagg cacaaaatgt ccggcgcgat ttctgcatat tcacaggcaa acagtttgcc
     2461 ggtacgacca aatccggtgg cgatctcgtc ggcaatcagc aagatacctt cgcgatcgca
     2521 cattttgcgg attcgtttta accattccgg atggtacatg cgcatcccgc ctgcgccctg
     2581 gacaatcggc tcaatgatca ccgctgcgat ttcatgacga tgcgccgcca tcaggcgggc
     2641 aaaaccaacc atatcgcgtt catcccattc accatccatg cggctttgcg gggcgggagc
     2701 aaacaagttt tctggcagat agcctttcca caggctgtgc attgagttgt ccggatcgca
     2761 caccgacatc gcgccaaagg tatcgccatg ataaccattg cggaaggtca gaaaacgctg
     2821 gcgcgcttcg cctttggctt gccagtactg caacgccatt ttcatcgcca cttccaccgc
     2881 tacggaaccg gagtccgcga gaaaaacgca ctccagcggt tgcggcgtca ttgccaccag
     2941 tttgcggcat agctcgatgg cgggcgcatg ggtgataccg ccaaacatca catgcgacat
     3001 ggcatcaatt tgcgtcttca tcgccgcatt aagctgcggg tgattgtagc cgtggatcgc
     3061 cgcccaccag gacgacatac cgtcaaccag gcgtctgccg tcagacaaaa ttagctcgca
     3121 accttcggcg ctcgccaccg gataaaccgg cagaggggag gtcatggatg tgtatgggtg
     3181 ccagatatgg cgttggtcaa aggcaagatc gtccgttgtc ataatcgact tgtaaaccaa
     3241 attgaaaaga tttaggttta caagcctaca ccgaattaac aacaaaaaac acgttttgga
     3301 gaagccccat ggctcaccgc ccacgctgga cattgtcgca agtcacagaa ttatttgaaa
     3361 aaccgttgct ggatctgctg tttgaagcgc agcaggtgca tcgccagcat ttcgaacctc
     3421 gtcaggtgca ggtcagcacg ttgctgtcga ttaagaccgg agcttgtccg gaagattgca
     3481 aatactgccc gcaaagctcg cgctacaaaa ccgggctgga agccgagcgg ttgatggaag
     3541 ttgaacaggt gctggagtcg gcgcgcaaag cgaaagcggc aggatcgacg cgcttctgta
     3601 tgggcgcggc atggaagaat ccccacgaac gcgatatgcc gtacctggaa caaatggtgc
     3661 agggggtaaa agcgatgggg ctggaggcgt gtatgacgct gggcacgttg agtgaatctc
     3721 aggcgcagcg cctcgcgaac gccgggctgg attactacaa ccacaacctg gacacctcgc
     3781 cggagtttta cggcaatatc atcaccacac gcacttatca ggaacgcctc gatacgctgg
     3841 aaaaagtgcg cgatgccggg atcaaagtct gctcaggtgg cattgtgggc ttaggcgaaa
     3901 cggtaaaaga tcgcgccgga ttattgctgc aactggcaaa cctgccgacg ccgccggaaa
     3961 gcgtaccaat caacatgctg gtgaaggtga aaggcacgcc gcttgccgat aacgatgacg
     4021 ttgatgcctt tgattttatt cgcaccattg ccgtagcgcg gatcatgatg ccgacctctt
     4081 acgtgcgcct ttctgccgga cgcgagcaga tgaacgaaca gactcaggcg atgtgcttta
     4141 tggcaggcgc aaactcgatt ttctacggtt gcaaactgct gaccacgccg aatccggaag
     4201 aagataaaga cctgcaactg ttccgcaaac tggggctaaa tccgcagcaa actgccgtgc
     4261 tggcagggga taacgaacaa cagcaacgtc ttggacaggc gctgatgacc ccggacaccg
     4321 acgaatatta caacgcggca gcattatgag ctggcaggag aaaatcaacg cggcgctcga
     4381 tgcgcggcgt gctgccgatg ccctgcgtcg ccgttatccg gtggcgcaag gagccggacg
     4441 ctggctggtg gcggatgatc gccagtatct gaacttttcc agtaacgatt atttaggttt
     4501 aagccatcat ccgcaaatta tccgtgcctg gaagcagggg gcggagcaat ttggcatcgg
     4561 tagcggcggc tccggtcacg tcagcggtta tagcgtggtg catcaggcgc tggaagaaga
     4621 actggccgag tggcttggct attcgcgggc gctgctgttt atctctggtt ttgccgcgaa
     4681 tcaggcagtc attgccgcga tgatggcgaa agaggaccgt attgttgccg accggcttag
     4741 ccatgcctca ttgctggagg ctgccagttt aagcccgtcg cagcttcgcc gttttactca
     4801 taacgatgtc gctcatctgg cgcgactgct tgcttccccc tgtccggggc agcaactggt
     4861 ggtgacagaa ggcgtgttca gcatggacgg cgatagcgcg ccactggcgg aaatccagca
     4921 ggtaacgcaa cagcacaatg gctggttgat ggtcgatgat gcccacggca cgggcgttat
     4981 cggggagcag gggcgcggca gctgctggct gcaaaaggta aaaccagaat tgctggtagt
     5041 gacttttggc aaaggatttg gcgtcagtgg ggctgcggtg ctttgctcaa gtacggtggc
     5101 ggattatctg ctgcaattcg cccgccacct tatctacagc accagtatgc cgcccgctca
     5161 ggcgcaggca ttacgtgcgt cgctggcggt cattcgccgt gatgaggggg atgcacggcg
     5221 cgaaaaactg gtgtcactca ttgcgcgttt tcgtgccgga gtacaggatt tgccgtttac
     5281 gctggctgat tcatgcagcg ccatccagcc attgatcgtc ggtgataaca gccgtgcgtt
     5341 acaactggca gaaaaactgc gccagcaagg ctgctgggtc acggcgattc gcccgccaac
     5401 cgtacccgct ggtactgcgc gactgcgctt aacgctaacc gctgcgcatg aaatgcagga
     5461 tatcgaccgt ctgctggagg tgctgcatgg caacggttaa taaacaagcc attgcagcgg
     5521 catttggtcg ggcagccgca cactatgagc aacatgcaga tctacagcgc cagagtgctg
     5581 acgccttact ggcaatgctt ccacagcgta aatacaccca cgtactggac gcgggttgtg
     5641 gacctggctg gatgagccgc cactggcggg aacgtcacgc gcaggtgacg gcgttagatc
     5701 tctcgccgcc aatgcttgcg caagcccgcc ataaagatgc cgcagatcat tatctggcag
     5761 gagatatcga atccctgccg ttagcgactg cgacgttcga tcttgcatgg agcaatctcg
     5821 cggtgcagtg gtgcggtaat ttatccacgg cactccgcga gctgtatcgg gtggtgcgcc
     5881 ccggaggcgt ggtcgcgttt accacgctgg tgcagggatc gttacccgaa ctgcatcagg
     5941 cgcggcaggc ggtggacgag cgtccgcatg ctaatcgctt tttaccgcca gatgaaatcg
     6001 aagagtcgct gaacggcgtg cgttatcaac atcatattca gctcatcaca ctatggtttg
     6061 atgatgcgct cagtgccatg cgttcgctga aaggcatcgg tgctacgcat cttcatgaag
     6121 ggcgcgaccc gcgaatatta acgcgttcgc agttgcagcg attgcaactg gcctggccgc
     6181 aacaacaggg gcgatatcct ctgacgtatc atcttttttt gggagtgatt gctcgtgagt
     6241 aaacgttatt ttgtcaccgg aacggatacc gaagtgggga aaaccgtcgc cagttgtgca
     6301 cttttacaag ccgcgaaggc agtaggttat cggacggcag gttataaacc ggtcgcctct
     6361 ggcagcgaaa agaccccgga aggcttacgt aatagtgacg cgctggcgtt acagcacaac
     6421 agcagcctgc agctggatta cgcaacggta aatccttaca ccttcgcaga atccacctcg
     6481 ccgcacatca tcagcgccca agagggcagg ccgatagaat cattggtaat gagcgccgga
     6541 ttacgcgcgc ttgaacaaca ggctgactgg gtgttagtgg aaggtgctgg cggctggttt
     6601 acgccgcttt ctgacacttt cacttttgca gattgggtaa cacaggaaca actgccggtg
     6661 atactggtag ttggtgtgaa actcggctgt attaatcacg cgatgttgac tgcgcaggca
     6721 atacaacacg ccggactgac tctggcgggt tgggtggcga acgatgttac gcctccggga
     6781 aaacgtcacg ctgaatatat gaccacgctc acccgcatga ttcccgcgcc gctgctggga
     6841 gagatcccct ggcttgcaga aaatccagaa aatgcggcaa ccggaaagta cataaacctt
     6901 gccttgttgt agccattctg tatttggtta aattgcgagc gagatcgcgt cttcgattga
     6961 ctgcaattta accaattaaa ttctaaaata atcacgaaaa aaattttact tccgcctcat
     7021 gcggcgaatg tgggaattgc ccaggcggcg ggggataggg gctggagaca gttatccact
     7081 attcctgtgg ataaccatgt gtattagagt tagaaaacac gaggcaagcg agagaatacg
     7141 cggcttgcac gcgaattggc gttaaagacg gctcaaagaa atatctttta ttttttaact
     7201 ggttagataa atgcaatggc agtcactgaa caggcatctc ttgccataaa actgtcatca
     7261 ctcatcttga caaatgttaa aaaagccgtt gctttgggga taacccggta aggctggagt
     7321 tttatctcgc cacagagtaa attttgctca tgattgacag cggagtttac gctgtatcag
     7381 aaatattatg gtgatgaact gtttttttat ccagtataat ttgttggcat aattaagtac
     7441 gacgagtaaa attacatacc tgcccgccca actccttcag gtagcgactc atgagtaaac
     7501 cgttcaaact gaattccgct tttaaacctt ctggcgatca gccagaggcg attcgacgtc
     7561 tcgaagaggg gctggaagat ggcctggcgc accagacgtt acttggcgtg actggctccg
     7621 ggaaaacctt caccattgcc aatgtcattg ctgaccttca gcgcccaacc atggtacttg
     7681 cgcccaacaa aacgctggcg gcccagctgt atggcgaaat gaaagagttc ttcccggaaa
     7741 acgcggtgga gtatttcgtc tcctactacg actactatca gccggaagcc tatgtaccga
     7801 gttccgacac cttcattgag aaagatgcct cggttaacga acatattgag cagatgcgtt
     7861 tgtccgccac caaagcgatg ctggagcggc gtgatgtgat tgtagtggcg tctgtttccg
     7921 cgatttatgg tctgggcgat cctgatttat atctcaagat gatgctccat ctcacggtcg
     7981 gtatgattat cgatcagcgc gcgattctgc gccgactggc ggagctgcaa tacgctcgta
     8041 atgatcaagc attccagcgt ggtactttcc gcgttcgtgg cgaggtgata gatatcttcc
     8101 cggcagaatc ggatgacatt gcacttcgcg tggaactgtt tgacgaggaa gtggaacgat
     8161 tgtcgttatt tgacccgctg accgggcaga ttgtttccac tattccacgt tttaccatct
     8221 acccgaaaac gcactacgtc acaccgcgcg agcgcatcgt acaggcgatg gaggagatca
     8281 aagaagaact ggccgccaga cgcaaagtgc tgttggaaaa caacaaactg ctggaagagc
     8341 agcggctgac ccagcgtacc cagtttgatc tggagatgat ggacgagctg ggctactgtt
     8401 cggggattga aaactactcg cgcttcctct ccggtcgtgg accgggtgag ccaccgccga
     8461 cgctgtttga ttacctgcct gccgatgggc tgctggtcgt cgatgaatct cacgtcacca
     8521 ttccacaaat tggcggcatg tatcgcggtg accgggcgcg taaagagaca ctggtggagt
     8581 acggcttccg cctgccatca gcgctggata accgtccgct taagtttgaa gagttcgaag
     8641 cattagcgcc gcaaaccatc tatgtttcgg cgacgccggg taattacgag ctggaaaaat
     8701 ccggcggcga tgtggtggat caggtggtgc gtccaaccgg attgcttgac ccgattatcg
     8761 aagtgcggcc ggtggcgaca caggttgatg atcttctttc ggagattcgt cagcgagcgg
     8821 caattaacga acgcgtgctg gtcaccacac tgaccaagcg aatggcggaa gatcttaccg
     8881 aatatctcga agaacacggt gagcgcgtgc gttatcttca ctcagatatc gacaccgtcg
     8941 agcgtatgga gattatccgc gacttgcgtc tgggtgagtt cgacgtattg gtagggatca
     9001 acttactgcg tgaaggtctg gatatgccgg aagtgtcgct ggtggcgatc ctcgacgctg
     9061 acaaagaagg cttcctgcgt tccgaacgtt cgttgatcca gaccattggt cgtgcggcac
     9121 gtaacgttaa cggtaaagcg attctctacg gcgataggag cactccatca atggcgaaag
     9181 cgattggcga aaccgaacgt cgccgtgaga aacagcagaa gtacaacgag gaacacggca
     9241 ttacgccgca aggcttgaac aagaaagtgg tcgatatcct ggcgctgggg cagaacattg
     9301 ccaaaaccaa agcgaagggc agaggaaaat cgcgcccgat tgttgagccg gataatgtgc
     9361 cgatggatat gttgccgaaa gcgttgcagc agaaaatcca tgaactggaa gggctgatga
     9421 tgcaacacgc gcagaatctg gagttcgaag aagcggcaca aattcgtgac cagttgcatc
     9481 agttgcgtga gttgtttatt gccgcatcgt aacaggatag cgaagaagac tgatgacaaa
     9541 cggaaaacag tctgatgcgc tacgcttatc aggcctacat tttctccgca atatattgaa
     9601 tttgcgcggt ttgtaggccg gataaggcgt tcacgccgca tccggcataa acaacgcgca
     9661 ctttgtcttc aataggaagc cggaattttc cttcggattt ccgttaacct aaagcctgta
     9721 acgccttttc cagcgcgtta tgtaacaact ggcggtcatg acgatacgga atatcgctgg
     9781 cctccagtac ctcctggatc acaatccgct ctttcaccgc cgagacatcc acttttggcc
     9841 cgacgatgac cgcatcaatg acctttttac caacatactg ctccataatt gccagcttgc
     9901 tttccagctt caaattagcc gcaggtaaac tcaactcacg ccccagattg ccgatataaa
     9961 ccatcggcgc tggcgtgcgg cgtaatgcct gggcgatttc cttcagcagc agaattggca
    10021 tcaggctggt ataaaaactg ccaggcccaa taatgatgag atccgcttca ttgatagcgt
    10081 gaaccgcctc acgcgttgcg ggtacattag gcgttaacaa taactcttga atcggcgtag
    10141 ttaactggtc gatattgacc tcgccgtaaa cttcatgccc ctgatcgtca atcgccatca
    10201 gatcaacagg atgctctgac attggaatca aatgcgtatc cactttcagc agattacgaa
    10261 ttaaattgat ggcttccaga ggccgcacgc taaggtgatc cagcgccttt aacatcaagt
    10321 ttccgagatt atgaccggaa agttcgccat tgccgccaaa acggtattca aacatcgcgg
    10381 aggcgacgtt cggttccgtt atcagctggt tgaggcagtt gcgcatatcg ccccaagcaa
    10441 tgccgccttc tgaacggcga atacgccccg tcgagccacc attatccgtg gtggtgacga
    10501 tacccgttaa acgagaaccc aaagacgaaa gtgatgagag aacgcgtccc agtccatgcc
    10561 ctccgccgag agcaacgaca cgatcaagat cagccagcgt acgattgcgc atatagtttc
    10621 ctggagtcag attatccgcg ctacagtagc gcaaattgcg gggaaacagc aattaacctg
    10681 ccaatattga ggatacaaac tattttcttc tatctctaaa ggacgatgca cgctatgcct
    10741 ccctgatgat gtatatcaaa gtaaaaccgc cattttccct tattctgtag cgaaatagca
    10801 cgatcatgac gctatatata tgattacata gcgaaagtgt ggatggtaaa aatctcattt
    10861 acacgctagt atcggcttaa ccactaaaca ctctagcctc tgcacctggg tcaactgata
    10921 cggtgctttg gccgtgacaa tgctcgtaaa gattgccacc agggcgaagg aagaaatgac
    10981 ttcgcctccc gtatctggaa aggtgtacat ggcttcacaa ctgactgatg tatttgcgcg
    11041 taagttttac tacttgcgcc tgtcgattac cgatgtgtgt aactttcgtt gcacctactg
    11101 cctgccggat ggctacaaac cgagcggcgt caccaataaa ggctttctta ccgtcgatga
    11161 aattcgccgg gttacgcgcg ccttcgccag tctgggcacc gaaaaagtgc gcctgacagg
    11221 aggagagccg tctttacgcc gcgactttac cgatatcatc gccgctgtgc gggaaaacga
    11281 cgctatccgc cagattgcgg tcaccaccaa tggttaccgt ctggaacgcg atgtggcgaa
    11341 ctggcgcgat gcgggactta ctggcattaa cgtcagtgtc gacagtttgg acgcccgcca
    11401 gtttcacgct attaccgggc aggataaatt caaccaggtc atggcaggga ttgatgctgc
    11461 atttgaggcc ggttttgaga aggtcaaagt caataccgtg ctgatgcgtg atgttaatca
    11521 tcaccagctc gacacctttc tgaactggat ccagcatcgc cctatccagc tgcgtttcat
    11581 cgaactgatg gaaacgggcg agggcagcga gctcttccgt aagcatcaca tctctggtca
    11641 ggttctgcgt gacgagctac tgcgtcgcgg ctggatccac caattacgtc aacgcagcga
    11701 cggtcccgcg caagtctttt gccatccaga ttacgccgga gagattggcc ttatcatgcc
    11761 gtatgaaaaa gacttctgcg ccacttgcaa ccgcctgcgc gtttcctcca ttggtaaact
    11821 ccatctctgc ctgtttggtg aaggcggcgt taacctgcgc gatctgctgg aaggcgatac
    11881 ccagcaacag gcgctggaag cgcgtatttc agcggcgctg cgggagaaga aacagaccca
    11941 tttcctgcat caaaacaaca ccggtattac gcaaaactta tcgtacattg gcggctaaaa
    12001 cgtcaaaagg agagatcaga tgagtcaggt aagcactgaa tttatcccga cccgtattgc
    12061 tattcttacg gtttctaatc gtcgcggtga agaagacgat acctccggtc actatctgcg
    12121 cgattcggcg caagaagcgg gccatcacgt tgtcgatgaa gccattgtga aagaaaaccg
    12181 ctacgctatt cgcgctcagg tatctgcgtg gatcgccagc gacgatgtac aagtggtatt
    12241 gattacgggt ggtactggcc tgacggaagg tgatcaggct cccgaagcgc tgctgccgtt
    12301 gttcgaccgt gaggttgaag gttttggtga agtgttccgt atgttgtcgt ttgaagagat
    12361 tggcacttcc acgttgcaat ctcgtgcggt agcgggcgtc gctaacaaaa cgctgatttt
    12421 cgccatgccg ggttcgacca aagcgtgccg taccgcatgg gaaaatatca tcgcgccgca
    12481 gctggatgcc cgtacgcgtc cgtgtaattt ccatccacat ttgaagaaat aagtatgtcg
    12541 caactgaccc atatcaacgc cgctggcgaa gcgcacatgg tggatgtctc cgccaaagcg
    12601 gaaaccgtgc gtgaagcgcg ggcggaagcc tttgtcacca tgcgcagcga gacgctggcg
    12661 atgattattg atggtcgcca ccacaaaggc gacgtatttg ccactgcgcg tattgccggt
    12721 attcaggcgg caaaacgcac ttgggatctg atcccgctgt gtcacccgct gatgctcagc
    12781 aaagttgaag tcaatttaca ggccgagccg gagcacaatc gggtgcgtat agaaacctta
    12841 tgccgcctga ccgggaaaac cggtgtcgaa atggaagcgt taaccgcggc ctccgtggcg
    12901 gcgctgacca tttatgacat gtgcaaagcg gtgcaaaaag atatggtgat tggtccggta
    12961 cgtttgctgg cgaagagcgg cggcaagtcg ggtgacttta aggtggaagc ggatgattaa
    13021 agttcttttt ttcgcccagg tgcgcgagtt ggtgggaaca gatgcaaccg aagtggctgc
    13081 ggattaccca accgttgaag cgttacgcca gcacctggct gcgcagagcg atcgctgggc
    13141 gctggcgctg gaagatggca aattgctggc tgccgtcaac cagacgctgg tgagttttga
    13201 ccatccgctg actgacggcg acgaagtagc tttcttcccg ccggtaaccg gaggttaaga
    13261 tggcagaaac caaaattgtt gtcggcccgc agccgttcag cgtaggagaa gagtacccgt
    13321 ggctggcgga gcgtgacgaa gacggtgcgg tagtcacctt caccggaaag gtgcgcaacc
    13381 ataacctggg cgacagcgtc aaagcattaa ccctcgaaca ctatccgggg atgactgaaa
    13441 aagcactggc agaaattgtt gatgaagcgc gtaaccgctg gccgctgggg cgcgtcacgg
    13501 tgattcaccg catcggggaa ttatggccgg gcgatgaaat cgtttttgtc ggtgtcacca
    13561 gtgcgcatcg cagcagtgcg tttgaagccg ggcagtttat tatggattat ctcaaaaccc
    13621 gcgcaccgtt ctggaagcgc gaagccacgc cggaaggcga ccgctgggtt gaagcccggg
    13681 agagcgatct gcaggcggca aaacgctggt agttttttgt tagccggata aggcaccgct
    13741 gcgtccggca atttcaccgc tcagtacatg cgtttacgct gcgtcgatgc acagcctcat
    13801 cattttgcag tatccttaag atattcctta tatcttcagg agatcgtcat ggacagattc
    13861 ccacgttctg attcaatcgt acaaccctgg gctggcttgc aaacttatat ggcgcaagtc
    13921 tatggctgga tgaccgttgg cttgttgctg accgcatttg ttgcctggta tgcggctaat
    13981 tccgcggccg tgatggagct gttgttcact aaccgtgtct ttttaatcgg tctgatcatc
    14041 gcgcaattag cattggttat tgtgttatca gcgatgattc aaaagctgag cgcaggtgta
    14101 acgacgatgc tctttatgct ttattcggcg ctgacgggtc ttacgctttc cagtatattc
    14161 attgtctata ccgctgcttc tatcgccagt actttcgtcg ttactgccgg gatgttcggc
    14221 gcaatgagcc tgtacggtta caccacgaag cgcgatttaa gtggctttgg caatatgctg
    14281 tttatggcgt taatcggcat tgtgctggca tcgctggtca acttctggct gaaaagcgaa
    14341 gcattgatgt gggcagttac ctacatcggc gtgattgtct ttgtcggatt gacggcgtat
    14401 gacactcaga aattgaaaaa tatgggtgag cagattgata cccgcgacac gtcgaacctg
    14461 cgcaaatatt ccattcttgg tgcgttaacc ttgtatctgg acttcatcaa cctgttcctg
    14521 atgttgttac ggatcttcgg caaccgccgt taattctttt tcaccatcac tatcccggcc
    14581 atgtgtcggg atttttttct tactaaaaac gacacttaac aagtaccagg tataaaatat
    14641 gccttatgag cgagcggaat cgttttccgt atccataaat acaaaagtat tttgtatgcg
    14701 tggttttaaa tttattgatg gtgaatatta atgttttttc taaggacaac taaaagaatg
    14761 gaatcatact cgcaaaacag caataaatta gattttcagc acgaggccag gatattaaac
    14821 ggtatatggc tcattaccgc tttaggtttg gtggcaaccg caggactagc ctggggagct
    14881 aagtatctcg aaattacggc aaccaaatat gattcaccac caatgtatgt cgccataggg
    14941 ttattattac tttgtatgta tggcttaagt aaggatatca acaagataaa tgccgccatc
    15001 gcgggcgtaa tatatctgtt tttactctct ttggtggcga ttgtcgttgc aagtttagtt
    15061 cctgtatatg ccattatcat cgtgttcagc actgcgggcg cgatgttttt aatcagtatg
    15121 ctggccggtt tattatttaa tgttgatcct ggttctcacc gttttatcat tatgatgacg
    15181 ttgacagggt tggccctggt aatcatcgtg aatgcggcat taatgagtga acggcccatt
    15241 tgggtaataa gttgcttaat gattgtgtta tggccaggca ttatctcgca tggacgaaat
    15301 aagctccttg aactggcagg aaaatgccat agtgaagagt tgtggagtcc ggttcgttgc
    15361 gcttttacag gtgcattaac actctattac tattttatcg gcttctttgg gatacttgcc
    15421 gcgatagcta taacgcttgt ctggcaaagg catacgcgtt tttttcatta gtcgtaaatg
    15481 cagatactaa agaacttaac taaacttcac atcgccgctt catttttcgc ccgtagcttc
    15541 ttcgcttggc tttccagtag caggtaacag atcagcgcca gcagcagcgg gataaagtaa
    15601 tacaggacgc gataagcgag cagggcggcg ataatgctcc ctttcgaggt atgctcccca
    15661 gccagtagtg cgataaacac cgcttccagc acaccgatcc ccgccggaat atgcacgatg
    15721 acgccagcaa tactactaac cagtaacacg cccagtacaa agaaatagtt cacgctttga
    15781 ccaagtaaca gccagataat cgcccccatt accatccagt taacgctgga aatcagcatc
    15841 tgagcgaggg cgaatttcca tgaaggcagc accagctttt gccctttgat ggtcatatgg
    15901 cggtgcttcg cgaaagcgca aaaccacaaa taaaccgcga taatcatcag taagccaatg
    15961 ccgagaatac gcagcgtagt ttgatcgaca taccagtaat ccggcaactc caccacgcct
    16021 gcggtaaaga taatccctgc cagtaaaata tagcccagcc agttggtggt aatactgagc
    16081 gaggaaatcc gcgtaatagt gctgcccggt aaccctagtc gggagtacaa acgatagcgc
    16141 ataccaatgc cgccgaccca ggtactgagc gtcaggttga aggcgtagca gataaacgac
    16201 accagcatca cctggcgctt cgccagtttg tgaccgcagt aaaagcgggc gagcaggtca
    16261 tagcagccgt aaatcagata gctgacgacc accagcccga ccgcactaag cagcgcaacg
    16321 cgattgtagt cgcggatgac cttccagacc tcttcccagt ccactttttt ggcgtagacc
    16381 accagcaaca caatcaccgc gataaaaaac agccaggtga ggatcttctt tgctaagcgc
    16441 cagcgcgggt gtgatttact catcaggggt ttacccccgt gttttcagtt tctacccgat
    16501 cctgcgtttc cattgtcggt tgtgcggggg gatcaacctg cgtcagacgt ggtgtgtgtg
    16561 ccggaagcca gccaaccagc gccgggaagt ggcgtaaaaa gtggaacgcc agcacgcttt
    16621 tggtcaggtt ccaccaggtg cgtttgggca gcatggtttc atccacctgc tgacaatctg
    16681 cggcaataat gccgttcaga ttatcgcgca gcgtctggtt aaaatgacga tcgtggatga
    16741 tgacatttgc ttcgagattc agtgacaaac tgagcggatc gagattactg gaccctactg
    16801 tcgcccagtg atcgtccatc aatgccactt tgccgtggag cgggcggcgg cggtactcaa
    16861 aaacctgaac gccgccttta accagatagt tatacagcaa gcgcgcaccg actctgacaa
    16921 tcggcatatc cggttcgccc tgaatgatca gtttgatccg caccccgcgc cgtgccgctt
    16981 tacgcaaggc gtgtaaaaat cgatagccgg ggaagaagta ggcgttggcg ataatcactt
    17041 cccgctgcgc ctgagtgagc attttcagat aatggcgttc aatatcatcg cgatgttctt
    17101 cgttatcgcg ccagaccagc aatacctgcg cttctcccgg ctggcggttc tcttccgctt
    17161 tgtgatgacg tcgccaccag cgtcgtgccg cgctctgtct aggcaggttt tccagctcaa
    17221 actggagaat atcttcgaca atcggccctt caaggcgtac cgcgtaatcc tgtttagcct
    17281 ctggaccgta gctggacata tgctcggcgg agtaattcag cccgccaata aaggcgatac
    17341 gcgcgtcgat caccacaatt ttgcgatgca tccggcgaaa cacattggtg cgcataccaa
    17401 aaaggcgagg gcggggatcg tagtagcgga acactacgcc agctgccgtc agttcattga
    17461 caaactcatc gctgagatcc ggcgaaccgt agccatccag caagacttcc gctttaaccc
    17521 cgcgttgcgc tgctgccagt agtgccgcat gcagctgttt gccgacgtta tcctcaaacc
    17581 agataaacgt ttcaagaatg atgcgttctt gtgcctcgcc aatcgcctta aacaccgcgg
    17641 gataatattg ctcgccgttt tccagcaact ggatcttatt gccttcgcgc cagctacatt
    17701 tcataaatga atctccgcac ttaaaggggc atgatcagaa aggtgtcgcc atgtccgcag
    17761 cggcaacgcg gttggcgcgc tggcgctggc atttttgacg tagatcctgt ccagtcgtag
    17821 tagaggaaat tgcaccggaa acgtgcgcgc cgggcgtccg tgggcgcggg taaaaatctc
    17881 atccagtccg gcctgcactt ttaacggatg attagctttt tgccgccagt cattgaaatc
    17941 acccgccacc aataccggtt cgccgtccgg tagctcattc acccattcgg cgagcatcgc
    18001 aagctgcgcc tgacggtgcg cctcacgcag gcccagatgt acgcacatca catgaatcgc
    18061 ttttccggtc atcggcggca caatgcggca gtagagcacg ccgcgctttt ccgcaccatc
    18121 gaccgaaaca tcgcgattct cataatgttc aatgggataa cgcgacagta cggcgttgcc
    18181 gtgatgccct tccgggtata cggcattgcg accgtaggca aaatcgctcc acatagtgtc
    18241 ggcgagaaac tcgtagtgcg aggtatcggg ccagttttcc acatgcagcg gatgaacttc
    18301 gtgcgcgccc atcacttcct gcaggcaaac aatatcggcg ctgacggtac gcacggcatc
    18361 gcgaagttcc ggcaaaatga agcgtcggtt aaacgcggta aagcctttgt gaatgttgat
    18421 ggtgagcacc ttgaacgaaa attgttgtgt ttgatcgggc ataattttcc tgtctttgcc
    18481 tcttatctca ttgaaatagt gtagtcggcg tcacaaaaag gtgcggtctt acggaatttt
    18541 ccgtaaagtt cggtactctg agtaagtaga gataaattct tcaggagaga agccatgaag
    18601 tggcaacaac gtgttcgtgt cgcaacgggt ctaagttgct ggcagattat gttgcattta
    18661 ctggtagtgg cgctgctggt ggtgggctgg atgagtaaga ctctggttca cgtcggcgtg
    18721 ggattatgcg cactgtattg tgtcacggta gtgatgatgc tggtgtttca gcgccacccc
    18781 gagcaacgct ggcgtgaggt ggcagacgtg ctggaagagc tgaccacgac ctggtatttt
    18841 ggcgcagcgc tgattgtgct gtggctgttg tcccgcgtac tggaaaacaa ctttttgctg
    18901 gcaattgcag ggctggcgat ccttgctggc ccggcggtag tgtctttgct ggcgaaagat
    18961 aagaagttac atcaccttac gtctaaacat cgcgtacgcc gctgaccctg tcgtggccgt
    19021 tatcaccagt agcggccaca aactattcca cacaatatcc agactcgcat ccttcaaata
    19081 aatctgcttg gtaatgtccg taaagtggcg aatagggtta atccacgtca gattttgcag
    19141 ccacactggc atgttttcca ccggcgaaac gtaaccggaa aggagaatgg cgggcatcat
    19201 aaaaacaaac acgccgataa acgcctgctg ttgtgtcgag cagagtgatg aaatcaacag
    19261 accgaatccc accagcgata aaccgtaaat caccatcgta aagtagaaca gcgctagcga
    19321 tccggcgaag gggatttgat acgcccagat accaatcgcc agcacaatgg tggcctggaa
    19381 ggtggcgaca attaacgccg gtacggcttt gccgatgaag atctgccagg tggtgagcgg
    19441 cgaaaccagt agctgatcga gcgtaccttg ttcacgttcg cgggcgacgg aaagtgaagt
    19501 gacgatcatt acgccgatag tggtgatcat ggcgatcagc gacggcacca caaaccattt
    19561 gtagtcgaga ttcgggttat accagttgcg taccaccagc tcgctgttgt tgggtttcgg
    19621 ttttccttcc agcagctcct gctgataatt tttgacgatc tgttgcaggt agttggcggc
    19681 aatttgcgca ctgttggagt tacgcccgtc gaggatcaac tgcaacggcg cggtctggaa
    19741 ggtatccagt ttgcgcgaga agtcagccgg gaaacgcacc agtagtaacg ccttttgtgt
    19801 gtcgatggtc gggcggatct cctgtgggct tttcagcagc agcacatgtg taaaggcgct
    19861 ggcgcgggca aaacgttgag taagttccac cgaatgctcg ccgttatctt catcgtagat
    19921 ggcgatggtg gcgttagtca cttccagcgt ggcggcgaac gggaacagga tcacctgaat
    19981 tagcacgggt aaaatcagaa tcgcgcgggt ttgtggttcg cgcagcaacg actgcaactc
    20041 tttgcggatt aacgtccata agcgatgaaa catgctcttc tccctaatcc agccgacgtt
    20101 tggttttcag ccacgtcagg ccgataaaca tcaccgccga agcgatcaaa aacagcacgt
    20161 ttaccaccag caccactgga atattcccgg cgaggaacag gctttgcagg gtgctgacga
    20221 aataacgagc gggaataatg tacgtcaccg cgcggatcac cgcgggcata ctgtcgatct
    20281 gaaaaataaa gccggaaagc ataatcgacg gcagaaaagc ggcgttcagg gcgacctgag
    20341 cggcattgaa ctggttgcgg gtaatcgtgg aaatcagcag ccccattccc agggtactga
    20401 gtaaaaacag gctggagata aaaaacagaa tcagcagcga accgcgatac ggcacgccga
    20461 gaataaacac tgacaccagc atacacagca acatcgccag catcccgaga aagtaataag
    20521 ggatcagctt acacagcagc agttccgtgc gggtaatctc cgtagagagc agagcctcca
    20581 tggtgccgcg ttcccattct cgcgccacca ccagcgaggt gagaatcgcg ccgatgaccg
    20641 tcatgataat ggtcaccgca cccgggataa taaagtgctg gcttatcgcc gccgggttaa
    20701 accagtagcg ggtttgtaca tcaataagcg gttcaaaagt ctgcccgttg tcctccgctc
    20761 gctgcatttg ccagatctgc cagatccctt cgacataccc ctgcacaaag ttagcggtat
    20821 tcggctcact gccgtcggtg atcacctgaa tcggtgcggt ggcgttggcg cgctccatct
    20881 gttcggcgaa atccacggga ataaccacca gaccgcgaat tttccccgac tgcattttgg
    20941 cgatcagttc ctgacggtta tcgctgatgg tggcgtcgat gtagggcgaa ccggtcatgg
    21001 tgtgggtgaa atccagcgcc gcttcgctac gctgttccag caaaatcccg acccgcagct
    21061 tgctggagtc gaggttaatg ccgtaaccaa aaataaacag cagtagcagt gggatcacta
    21121 ccgcaatcag ccagctactc ggatcgcgaa cgatctgccg cgtctctttg acgcacagcg
    21181 cccgcacgcg atgccaggaa aggatcgggt tactcattgc tatgctcctt atcccagtcg
    21241 tggatcaact gaataaaggc ttgctccatg gtgggatcgg gttgctcatc gttagctgac
    21301 tgtgctttca aatcgtccgg cgtaccgctg gcgattaatt tcccacggta caccaggccg
    21361 atgcggtcgc aatattccgc ttcatccata aagtgggtgg tgaccatcac cgtgacgcct
    21421 ttctctacca tgctgttgat gtgcagccaa aattcacggc gggtgagggg gtcaacgccg
    21481 gaagtcggtt cgtcgagaaa gagaatatcc ggttcatgca tcagcgaaca ggccagcgcc
    21541 agccgctgtt taaaacctaa tggcagttca tcggtggcgt gtgaggcaat acttttcagg
    21601 ccaaatgcct cgctcatacg ggagattttt tcgttctgcg cccgaccgcg taagccatac
    21661 acaccagaga aaaagcgtaa attctgttcg accgtcaggt tgccgtagag cgaaaatttt
    21721 tgcgccatat agccgagatg ctggcgcgct ttaccggaac tctctttcag atccatcccc
    21781 agcaccagcg cctggccgga agtcggcacc agcaaaccgc acatcatctt aaaggtggtc
    21841 gatttacccg cgccgtttgg ccccagcaaa ccaaaaatct ccccacgttt aacggcaaag
    21901 ttgacgtgat cggtggcggc aaaatcacca aatttcttgg tcagttcttt cgcttcgatc
    21961 accgtctcgc cgggtgtgcc ttctaccgta tgcaatattg cacccagcgg cgattctgag
    22021 gttccggcac cgcccagcaa atcaataaac gcatcttcaa aacgcggcgt agtttcgttg
    22081 atgttgattt ccggcatccc gtcggcatgg cgaatatcgt ctggtgtggc ctcttttttg
    22141 aggatcagac gtaccgattt cccctgaatc atgccatcgc tgacctgcgg cagtttcaag
    22201 gcgcgttgca acagtttgcg gttgccctcg tgtggactgg tcatcagaaa gctgcgtccg
    22261 gccatggttt gtgtcagggc ttttggttct ccctgataca gcaactcgcc ttcgttcatc
    22321 agtaacacgt cacggcactg ctcggcttcg tcgagatacg aggtactcca gaggattaac
    22381 atcccttcgc ccgccagctc atgcaccatc tgccacagtt cgcgccgtga gatagggtca
    22441 acgccgacgc cgggttcatc gagcagcaac actttcggtt cgcccaccag ggtacaggcc
    22501 aggccgagtt tttgtttcat cccaccggag agcttgcccg ccaggcgtcc ggtaaacggc
    22561 ccaagagacg taaactccag caggcgagca aaagtttgct tacgtgcctc gccggtgacg
    22621 ctgcgcaaat ccgcgtacag attgaggttc tccatcaccg tgagatcttc atacagacca
    22681 aatttctgcg gcatatagcc gagcacggcg tgcagcgcgc cgtcgttttt gatcggatca
    22741 aagccaatca ccgtggcact gccgctgtcg ggtttcagta atcccgccaa catccgcatc
    22801 agtgtggttt tacctgcacc gtccggcccc accaaccccg tcacataacc ggcgtgaatg
    22861 gtacaatcga gcggcgcgac ggcgggcttg tccatgcccg gaaagcgttt ttccaggccg
    22921 ttcagcgtga taacggcatc attcatgtcc tgcctcgtca ccgaattgta ccgtcactgg
    22981 cattccctgg cgtaacgcat catcggcgtc ggtcaccaca atacgcaggc gatagacgag
    23041 gtcggtacgc agatccggtg tttcgacggt tttcggggta aattcagcag tcggcgaaac
    23101 gaaaccaatc tgcccgtgat acggcttgtc cgggcgacca tcggtataaa gcagcacttt
    23161 gcgccccggc tgggcctggt caagattccg ttcatcaaca taagcgcgca cccacaccgg
    23221 acgcgttagt gaaacggtaa acaccgtgcc accttcattg agaaccgtgc ctggctctac
    23281 cgcgcgcgtt aacagcgtgc catcagacgg tgctatcaac gttgagtcct gtaaattcag
    23341 ctccgcctgc gccagttgcg cctgcgcctg ttcgaggctg gctttcgcct gagcgatgtc
    23401 ctgttcacgg ttaccggaac ggtactgacg caatttatcc tgtgctgatt tcagcgttgc
    23461 ctgtgcctgg tcgcgcgagg agcgggcatt ttccaggtca tttgccgaaa tggtgcggct
    23521 tttccacagt ccttgctggc ggttatagaa gttctgcgca tagtcatagg cggcttgcgc
    23581 ctgtttcacc gctgcggcgg cctgagcgat ttcttcatcg cgatacccgg caagcatcag
    23641 atcatactgc gcctgtgcca ccgagacgcc cgctttcgcc tgcatcaggg caatctcata
    23701 cggcttgtga tccagttcgc ccagcacctg gcccgctttg atagcatcac cttcgtccac
    23761 cgccagcgat tcaacgcgcc ccccaacacg gaaactaaga ttcaccgtac gaatatccac
    23821 gttgccatac agcgtcaggc cgttatcctg gcggctttga taccaccagt agcctccggc
    23881 aaccacggcg gcaagtacca ctaccgccag tccgatcacg acaggttttt tcatcactac
    23941 aaactccttt gcgataatcc ttgcaaaatc aggtcgatat gacaggtcac cgtctggttg
    24001 atcagttcgg tcttttcttc atcgaacgcg gtccagccgg tacgtaacag aattgtttct
    24061 ttgccaagac ggaacgccag aatctcacca atcaacgcat gggtatgaag gatcatgcgg
    24121 gtgtcattag cgtcgcagcc ggtccaggcg gcaatcaggc gtgtcaggtg gctgtgtagc
    24181 ggactaatca cctgttcgtg caccaggtgg taggctgccg tgggagagag ctgctcacgg
    24241 gagataaact tgctgaggtt gacggtgtca tcctgggtga gcagcttaat catgttcctg
    24301 caggcgcgaa ggatcagttc acggatggca gcccgatcag gctgtggttg ggcgaacaag
    24361 cgttcggctt cctcggcatg cggacggaac tgctcgccaa taaaatcggc aatccactgg
    24421 gcgcaggcga ggtacaaatc ttctttcgaa ccgaagtagt aggtgatggc agcgatattc
    24481 tgcccggcct gggcggctat ctcgcgggtg gtggcgttca ttccatattc accaaactgc
    24541 gccagtgcgg cagcaatcag ctgttttttc gcctgttcac ccttgattgt catggcagga
    24601 ttattcatcg cacagtccat tcttaatcaa atgattgatt aagattatga ctccatatgg
    24661 gagttgtcca gtatggctaa gaattttagc tacgccagtc acagggataa tttatgcgct
    24721 gcgtcacaaa aactgctaca ctccgctccc tcatgacatt gtggtttttg tcattttcct
    24781 tttcagtatc tccctgaaaa ctacaccggt aacggtcggg gcggttcgga gtagttatgt
    24841 ctttcgattc tttgggttta agccctgata tcctgcgcgc cgttgccgag cagggttacc
    24901 gtgaacccac ccctattcag cagcaggcga tccctgctgt gctggaaggc cgcgacctga
    24961 tggctagcgc ccagaccggc accggcaaaa cagcgggctt tacgctgccg ctgttgcaac
    25021 acctgatcac tcgccagccg cacgccaaag ggcgtcgtcc ggtacgtgcg ctcattctta
    25081 ccccgacccg tgaactggcg gcacagattg gcgaaaacgt ccgtgattac agcaaatacc
    25141 tgaacattcg ttcgctggtg gtgtttggcg gtgtcagcat taacccacaa atgatgaagt
    25201 tgcgcggcgg cgttgatgtg ctggtggcaa cgccgggacg tttgctggac ctggaacatc
    25261 aaaatgcagt gaagctggat caggttgaaa tcctcgtcct cgatgaagct gaccgcatgc
    25321 tcgacatggg ctttatccac gatatccgtc gcgtgttaac aaaactgcca gcgaaacgcc
    25381 agaacctgct gttctctgcg accttctctg acgatattaa agccctggcg gaaaaactgt
    25441 tgcacaaccc gctggaaatc gaagtggcac gccgcaatac cgcgtctgat caggtgaccc
    25501 agcacgttca ctttgtcgat aagaaacgca aacgcgaatt gctgtcgcac atgattggta
    25561 aagggaactg gcagcaggtg ctggtgttta cccgtaccaa acacggcgct aaccatctgg
    25621 ctgagcagct caataaagat ggcatccgta gtgtggcgat ccacgggaat aaatcgcaag
    25681 gtgcgcgtac ccgtgcgctg gctgatttta aatcgggtga cattcgcgta ctggtggcga
    25741 ccgatatcgc tgcgcgcggt ctggatatcg aagagttgcc gcacgtcgtc aactatgaac
    25801 tgccaaacgt accggaagat tatgtccacc gtatcgggcg taccggtcgt gcggctgcta
    25861 ccggtgaagc gttgtcgctg gtgtgtgttg atgaacacaa actgctgcgt gatatcgaaa
    25921 aactgctgaa aaaagagatc ccgcgcattg cgattccggg ctatgagccg gacccgtcaa
    25981 tcaaagccga accgatccag aacggtcgcc agcaacgtgg cggcatcagg ctgatgaaca
    26041 aacgcaaaac tgcctgatgc gctacgctta tcaggcctac gtgaactctg caatatattg
    26101 aatttgcatg cttttgtagg ccggataagg cgtttacgcc gcatccggca tttcacaaca
    26161 agcacttgtc agcaatttga gaacacggga aaaaatttat ctgttttacc gcccataagc
    26221 caccgagacg ttacttctct atagccaatt gttcgcgcaa ctccattaaa aggtagccca
    26281 gtcgattctt gcccttacca ttaccaccgt ctccccagta agcatcgttt tccgtatgct
    26341 caaccagttt tgcgggcgcg gttgccagca agagcacacg cagttctgca tgctgttcga
    26401 atttagcgcg aagtgcttta cgcatcacct gttctttaac cgactcccag tttttacgaa
    26461 gtggcttgga acgattacgc cccatgcgag cggcgaccat gggggaagaa acccgacgga
    26521 tctcttcgcg gtatttttca tcaaggaatt tttgtgcctg aaaatagtgt tcggaggtgg
    26581 gccaggtatt cccgtcaacc ttgatgggcc tggcggcaaa gttagaaaaa tcaccgtagt
    26641 catcgctggt gctgtagaaa tttatgatgg tgtcttgcat gacgtgttgg atcctttgtg
    26701 ctcgaacggg cattaaaccg cattatgttg gtggttattg cgagccgctt tccagaaaca
    26761 gaaaaaccat tacccctgaa aaccgaaaaa tgccacaata ttggctgttt atacagtatt
    26821 tcaggttttc tcatggcatt aaccgccgcg cttaaagcgc aaattgccgc ctggtataag
    26881 gcgcttcagg aacagatccc cgactttatt ccccgtgcgc cgcagcggca gatgattgcg
    26941 gacgtcgcca aaacgctggc cggagaagaa gggcggcatc tggcgattga agcccccacc
    27001 ggcgttggga aaacgctctc ctatttgatc cccggcatcg ccattgcccg cgaagagcaa
    27061 aaaacgctgg tggtgagtac cgccaacgtg gcgttgcagg atcagattta cagcaaagat
    27121 ttaccgctgc tgaaaaagat cattcccgat cttaaattca ctgccgcttt cgggcgtggg
    27181 cgctacgttt gtccacgtaa tctgacggcg ctcgccagca ctgaacccac gcaacaggat
    27241 ctgctggcgt ttcttgacga cgaactgacg ccaaataatc aggaagagca aaaacgttgt
    27301 gcgaagctga agggcgatct cgacacttat aaatgggatg gtctgcgcga tcatacggat
    27361 atcgctatag atgatgatct ctggcgtcgt ttgagtaccg acaaagccag ctgcctcaac
    27421 cgtaactgtt actactatcg tgaatgcccg ttttttgtta ctcgtcggga gattcaggaa
    27481 gcggaagtgg tggtggcaaa ccatgcgctg gtgatggcgg cgatggaaag cgaagccgta
    27541 ctgcctgacc cgaaaaatct gctgctggtg ctggacgaag gtcatcacct gccagatgtg
    27601 gcccgggatg cgctggagat gagcgctgaa atcaccgtgc cctggtatcg actacagctg
    27661 gacttgttca cgaaactggt cgctacctgc atggagcagt ttcgcccgaa gaccatccca
    27721 ccgctggcga tccctgaacg tttgaatgcg cattgtgaag agttgtatga gcttatcgcc
    27781 tcgttaaaca acattctcaa tctctacatg cctgccgggc aggaggcaga gcaccgtttt
    27841 gcgatgggcg aactgcctga tgaattgctg gagatctgcc agcggctggc aaaactcacc
    27901 gagatgctgc gtggtctggc ggagttattt cttaacgatt taagtgagaa aaccggcagc
    27961 cacgatattg tgcgtctgca tcggttgatt ttacagatga accgcgcgct ggggatgttt
    28021 gaggtgcaaa gcaaactctg gcggctggcg tctctggcgc aatcttccgg tgcgccggtg
    28081 accaaatggg cgacgcggga agagcgcgaa gggcagctac acctctggtt tcactgcgtg
    28141 ggaatacgtg tcagcgatca gctggaaagg ctgctgtggc gcagtattcc gcacattatt
    28201 gtcacctccg caaccttgcg ttcgctgaat agtttttcgc gtttgcagga gatgagtggt
    28261 ctgaaagaga aagcgggcga ccgttttgtg gcgctggatt ccccctttaa ccactgcgaa
    28321 cagggcaaaa ttgttattcc ccggatgcgc tttgagcctt ccatcgacaa cgaagagcaa
    28381 cacattgccg aaatggcggc ctttttccgt gaacaggtgg agagcaaaaa atatctcggt
    28441 atgttggtac tgtttgccag cggacgggcg atgcagcgct ttctcgacta tgtgacggat
    28501 ttacgcctga tgttgctggt gcagggcgat cagccgcgtt atcgcttagt tgaactgcac
    28561 cgcaaacgcg ttgccaacgg tgagcgtagc gtgctggtgg gcttacagtc atttgccgaa
    28621 gggcttgatt tgaaaggtga tctgctcagc caggtgcata tccacaaaat cgcttttccg
    28681 cccatcgaca gcccggtggt gatcaccgaa ggggaatggc tgaaaagcct caaccgctat
    28741 ccgtttgagg tgcaaagcct gccgagcgcc tcgtttaacc tgattcagca ggttgggcga
    28801 ctgattcgaa gccacggttg ctggggcgaa gtggttatct acgacaaacg cttgctgaca
    28861 aaaaattatg gcaagcgact actggatgca ttaccggtat ttccgataga gcaaccggaa
    28921 gtccctgaag gtatagttaa aaagaaagaa aaaacgaaat ccccacgccg tcggcggcgt
    28981 taatgatgtg agtcaggtaa ggagtcgtaa atggactatc gcaaaatcat taaagagatc
    29041 gggcgcggga aaaaccacgc gcgcgattta gaccgggata ctgcgcgcgg tctgtatgct
    29101 catatgctca atggtgaagt ccctgacctt gagttgggcg gcgtattgat tgcgctgcgt
    29161 atcaaagggg aaggggaagc agagatgctc ggcttttacg aagccatgca aaatcacacc
    29221 atcaagctga cgccgcctgc aggcaagccg atgccgattg tcatccccag ttacaacggc
    29281 gcgcgtaaac aggctaacct gacgccatta ttggcgattc tgctgcataa actcggtttt
    29341 cctgtggtgg ttcacggagt tagcgaagat ccaactcgcg tgctgacaga aaccattttc
    29401 gaactgatgg gtattacacc aacgttgcac ggcgggcagg cgcaggcgaa gctcgacgaa
    29461 catcaaccgg tgtttatgcc tgtcggcgcg ttttgcccgc cgctggaaaa acaactggcg
    29521 atgcgctggc ggatgggcgt gcgtaacagt gcacataccc tggcgaaact ggcgacgcca
    29581 tttgccgaag gtgaggcact gtgtctttcc agcgtttcgc atccggaata cattggacgc
    29641 gtcgcgaagt tctttagcga tatcggcggg tgggcgttac taatgcatgg tactgaaggc
    29701 gaagtgtatg ctaatccgca gcgctgcccg cagatcaatc tcattgaccg tgaagggatg
    29761 cgggtgctgt atgaaaaaca ggaccctgct ggtagcgagt tactgccaca agcaaaagat
    29821 ccggaaacca cggcgcagtg gactgagcgt tgcctggctg gcagcgaagc gattcccgaa
    29881 tcgctgaaaa tccagatggc ttgctgcctg gtggctacgg gtgaagcggc aactatcagc
    29941 gatggcctgg cgcgcgttaa tcaggcattt taatttttat ctcagccgga tgagccacgt
    30001 catccggctt ttaccccacc gcaatatgaa attcctgcat ctttattgac cttcccgcgc
    30061 ccggcgtgca gcataaaaaa acaacaaaca cataacataa acaggagtta accatggaaa
    30121 gtggtcatcg ctttgatgct cagacgctgc acagttttat tcaggctgta tttcgtcaga
    30181 tgggtagcga ggaacaagaa gcgaaattag ttgccgatca tttaatcgcg gcaaacctgg
    30241 cagggcatga ttctcatggt attggcatga tcccaagcta tgtgcgttcc tggagtcagg
    30301 ggcatctgca aattaaccat catgccaaaa tcgttaaaga ggcgggggcg gcagtcacgc
    30361 tcgatggcga tcgcgcattc ggtcaggtcg cggcacacga agcgatggcg ctggggattg
    30421 agaaagcgca tcagcacggt attgctgccg tggcgctaca taactcgcat catatcggcc
    30481 gtatcggtta ctgggcggag cagtgttcag cggcggggtt tgtctctatc cactttgtta
    30541 gcgtggtcgg tattccaatg gtcgcgccgt tccacggtcg cgacagccgc tttggcacca
    30601 atcccttctg tgtggttttc cctcgtaaag ataatttccc gctgttgctt gattacgcca
    30661 ccagcgccat tgcatttggc aaaacccgcg tcgcctggca taaaggcgtc cccgtgccgc
    30721 caggttgcct gattgacgtt aacggcgtgc cgacgaccaa tccggcggta atgcaggagt
    30781 cgccgttggg gtcgctgttg acctttgccg aacataaagg ctacgccctt gcagcgatgt
    30841 gtgaaattct tggcggggcg ctttctggcg gtaaaacgac gcatcaggaa acgttgcaaa
    30901 ccagccccga tgccattctt aactgcatga ccactatcat catcaacccg gaactgttcg
    30961 gcgcgccgga ttgtaacgcg cagaccgaag cctttgccga gtgggtgaaa gcctcgccgc
    31021 atgatgacga taagccgatt ttgctaccgg gcgagtggga agtgaacacg cgtcgcgaac
    31081 ggcaggagca ggggattcca ttggatgcgg gaagctggca ggccatttgt gatgcggcgc
    31141 ggcagattgg tatgtcggaa gagacgttgc aggctttctg tcagcagtta gccagctaaa
    31201 aaaaagcccg tccagtggcg gacgggcaaa caagggtaac ataggatcaa tgagggttag
    31261 agcatatgcg tctgtcggca aacagacagg gaaatacttg tgctggacgt agcgtaaacg
    31321 cctgatccgt ccaacggttc ggattttgta ggcctgataa gacgcggtag cgtcgcatca
    31381 ggcatctgtt gccggatgcg gcataaacgc cttatccgtc ctacgaatta cttataaatt
    31441 accgcagtac cgcttaattt attgttgttg gtggcggaag tgatgctgta tccactggca
    31501 ccggctgccg ccgctttctc agccagtttc gcttccaggg catcgagggt ggatgcgcca
    31561 tcggcagaaa ccacgccgat tttattcatg ttctgtgcct gggatgccgt taccggttcc
    31621 gccgcgaaca cgccaaatga cagagttgaa agagccatag cagcaacaac agtattgata
    31681 gttttcataa ttaatctctc gcaggtgatt tttgtataag tgacgttgtt tcgtcgatgt
    31741 gatgagtatc acgttttttt acgagagata aaatcgaaga gaattgaccg ccttgttcaa
    31801 ataaattgat tgatatctaa catactgata aaccatttat ttgttgtact tatggacttg
    31861 ttttatgccg cgtggcagtc acagcagcgt taaaggcacg gaatgccacg caaaatacaa
    31921 attatactcg ctatgcatct cgccagttgt agttaacgta actgtctgtc tttcgaacct
    31981 ctgcgattat atcctgtata cgctggtttt tgtaaatctt tctcctgctg acaatgaata
    32041 cataagcgca cgctaggaat ggcttcccga cgggcttgcg ggatgggggc accgcactct
    32101 tcacattcat acaggctttc gccacgcgga atttcacccc gagcgcgggc aatcgcatct
    32161 tcaattgtac tgttgatctg ttcgttgacg gcgtcatcgt tagcccaagc ggatgccata
    32221 tcgtcctccc catatcaatg cttgtacagt taagtgtagc taatataggg acgaacctgg
    32281 gcagttcaag cgtcagatct ccgaccattc ccgcagcaga ttatgataaa gattgagcag
    32341 cgacaggatc tcttcatttt caccatagcg gcttttcagc gactgaatat ttttgtccag
    32401 ttcaaacagc atggcgcgct ttttatcatc gcggatcatc gactggatcc acataaatga
    32461 tgccactcgt acgccgcggg ttacgggtgt cacgcaatgc aggctgctgg agggatacaa
    32521 caccagatca cctgccggga gttttacccg atgttgtcca aaggtgtcat taacgaccag
    32581 ttcgccgccg tcgtagcttt gtggatcgct taaaaacagc gtggcagaaa ggtcagtgcg
    32641 catccagccg ttttgtggat ggctgcgtac tgcgccatcc acatggaaac cataggtttc
    32701 attgttctga tagcgattaa acagcggcgt ggaaagggta cgcggcaagg cagcggcaaa
    32761 gaacaatgca tgttggttaa ccgcgttcag cacctcattt tgcagtgcgg cgtataacgc
    32821 gctgcgggtg tctacttgct gattgttctt aacttgtgca ccttgtgctc cggtggtgac
    32881 gcgtccatcc acccattcgg cttgttccag ttgttcgcga aaatgggcga cgtcctgtgg
    32941 cgataacacg ccgggaatgt ggtacatcat acaagttctc caaaaagtgg ggcctgcgcc
    33001 ccacatctga atcagaaatg catattggct gtgagcaaga aggttcttgg ctcgcccggg
    33061 tgataacggt agccgctctt gttgattgag gcgacgtaat cggtatcaaa caggttgtaa
    33121 acgtttagct ggaagtcgag attgcgatta acccgatacc ccagtttggc atcggcgacc
    33181 cagtaacctt ctgtaaatgc tggcgttccc accgcgccgt ctgaaccttt atgtatactg
    33241 ccgatatagc gtgcgcccgc gccaacagag atatcatcgg ttgcctgata ttggctccat
    33301 aaggtgaagg cgtgctccgg ggtatacggc agcgatgagg aaccatcctg ggcaacatct
    33361 ttgccgtttt tgatggttgc tttttgctgg gtatagccgc caatcacctg ccacgcggga
    33421 gtgatattcc ctgccacgga tatctcatag ccttcgacgc gtttcttacc gtattgcgag
    33481 taagtcccgt catcattttg ctcaacttca ttttcgatat cagtacggaa cagcgcggcg
    33541 gtgagcaaca ggcgtttatc cagaacctgc catttggtgc caatctcgct ggtgttggct
    33601 ttttgcggtt taaaatcggt gcggttggca ctgttaccac tgccagactg cgcaagggcg
    33661 aagttgttgc cgcccggagg ctgctgggaa acggcatagt taatatagac attgccgttt
    33721 tccgtcaggt ggtacagcgc cccggctttc cagttcacca gattgcccga cttggcggtg
    33781 tcgacggtgg tgactggaga accttttgcc acaccagctg ggcaggtgat ggcaccgcgt
    33841 ccgctgccgc cgcaggcggt ggcactgtca tattcagtat gataattatc cagacggatc
    33901 ccgccgttca gctcaaaatc acgggtgatt tgcagcgtat cgaaggcgta aattgcgaag
    33961 gtatccgtct gaccattggc gtttgcgccg ttgcgcgtca ggccgccggg atgaattctg
    34021 ctgtcaggat gataaatatt taccgccggt aacgtcaccg gattaacgcc atagttagtc
    34081 tgcgtttcac gggtaaattc cacgccggta ctgacatcat gaccgataga accggtatag
    34141 aacgtcgagg tcaggttggt ctggttggtg agaattttat tactcacatc tttggtattc
    34201 gccgtgcgtg actaggtcca gctattcaca tcgctggtgg gctgagtaat attcgacgcc
    34261 ccgcccataa tcgccgtcat caggtaatcc tgctttacgc gcgaccaacg ggtagtattg
    34321 cgaatggtgg tgttatcgtt gatgtcgtgc tcaaaacgca tggtggcggt gtcggtggtc
    34381 gaatcgtcgt aatcggaatc cgtgccgtaa aagttatgag tatcaacttt tccggaatga
    34441 ttcggggccg ccgctcccgc agatggggca gaatagcccg gcaaaccgat ggtcggaatg
    34501 ccgccgtctg gcgtgttgtg ctgagtgaca tgcagataat taagatacaa acgatttgct
    34561 gtaccaaggc caaaagcgac agaaggggcg acgccgtaac gctcattttt gactttgtcg
    34621 cgtccggcat cgtgggtttt ttcgcccatc acattcaggc gcaccgcggt agtatcacca
    34681 atgacctgat tgacgtccag cgtgccgcgg cggaaccagg cgctgccaat actggcggag
    34741 gcgtcaatgc cggaatcatt gcgcggctgc ttgctgatca tattgatcga gcctgtcggt
    34801 gcgctgcgcc cgtagtcggt gccggacggc cctttaatca cttcgacctg ctcggtattg
    34861 aaggtgtcgc gcgagacgct gccgatatcg cgaataccat caatataaat actgttagag
    34921 gtatcggcac cacgcatata aatggcgtcg ccagtggtgg agttaccgtt ctcacccgca
    34981 aaaaacgcac ccacgc
//
LOCUS       DAEBXN010000033        34903 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.033, whole genome shotgun sequence.
ACCESSION   DAEBXN010000033 DAEBXN010000000
VERSION     DAEBXN010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34903
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.033"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            858..3143
                     /gene="nrdA"
                     /locus_tag="KJL99_001678"
     CDS             858..3143
                     /gene="nrdA"
                     /locus_tag="KJL99_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HBD4436437.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPTIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            3377..4507
                     /gene="nrdB"
                     /locus_tag="KJL99_001679"
     CDS             3377..4507
                     /gene="nrdB"
                     /locus_tag="KJL99_001679"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HBD4436438.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4507..4761
                     /gene="yfaE"
                     /locus_tag="KJL99_001680"
     CDS             4507..4761
                     /gene="yfaE"
                     /locus_tag="KJL99_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HBD4436439.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4815..5465)
                     /gene="inaA"
                     /locus_tag="KJL99_001681"
     CDS             complement(4815..5465)
                     /gene="inaA"
                     /locus_tag="KJL99_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HBD4436440.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            5644..5886
                     /locus_tag="KJL99_001682"
                     /pseudo
     CDS             5644..5886
                     /locus_tag="KJL99_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(5928..7004)
                     /gene="glpQ"
                     /locus_tag="KJL99_001683"
     CDS             complement(5928..7004)
                     /gene="glpQ"
                     /locus_tag="KJL99_001683"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBD4436441.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(7009..8367)
                     /gene="glpT"
                     /locus_tag="KJL99_001684"
     CDS             complement(7009..8367)
                     /gene="glpT"
                     /locus_tag="KJL99_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HBD4436442.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            8640..10268
                     /gene="glpA"
                     /locus_tag="KJL99_001685"
     CDS             8640..10268
                     /gene="glpA"
                     /locus_tag="KJL99_001685"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HBD4436443.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            10258..11517
                     /gene="glpB"
                     /locus_tag="KJL99_001686"
     CDS             10258..11517
                     /gene="glpB"
                     /locus_tag="KJL99_001686"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HBD4436444.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            11514..12704
                     /gene="glpC"
                     /locus_tag="KJL99_001687"
     CDS             11514..12704
                     /gene="glpC"
                     /locus_tag="KJL99_001687"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HBD4436445.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            12897..13847
                     /locus_tag="KJL99_001688"
     CDS             12897..13847
                     /locus_tag="KJL99_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HBD4436446.1"
                     /translation="MTESTTSSPHDAVFKTFIFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRPMRYATAA
                     MQSHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDHDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKLVGLQQGKLEGLQ
                     EGMHEQAIKIALRMLEQGIDRDQVLAATQLSEADLAANNH"
     gene            complement(13888..14358)
                     /locus_tag="KJL99_001689"
     CDS             complement(13888..14358)
                     /locus_tag="KJL99_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HBD4436447.1"
                     /translation="MSNPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESK
                     TALDNLDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAA
                     GFLAVAPDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(14413..15615)
                     /locus_tag="KJL99_001690"
     CDS             complement(14413..15615)
                     /locus_tag="KJL99_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HBD4436448.1"
                     /translation="MLKVEMLSTGDEVLYGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVIPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(15702..16244)
                     /locus_tag="KJL99_001691"
     CDS             complement(15702..16244)
                     /locus_tag="KJL99_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4436449.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGLYVGVNASF"
     gene            16523..16948
                     /gene="nudI"
                     /locus_tag="KJL99_001692"
     CDS             16523..16948
                     /gene="nudI"
                     /locus_tag="KJL99_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HBD4436450.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(16987..17589)
                     /locus_tag="KJL99_001693"
     CDS             complement(16987..17589)
                     /locus_tag="KJL99_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBD4436451.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADISDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQNKAPDKNIVIFTHNH
                     CLTYIAKNKRDAIFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            17897..19036
                     /gene="arnB"
                     /locus_tag="KJL99_001694"
     CDS             17897..19036
                     /gene="arnB"
                     /locus_tag="KJL99_001694"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HBD4436452.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            19040..20008
                     /gene="arnC"
                     /locus_tag="KJL99_001695"
     CDS             19040..20008
                     /gene="arnC"
                     /locus_tag="KJL99_001695"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HBD4436453.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILINRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            20008..21989
                     /gene="arnA"
                     /locus_tag="KJL99_001696"
                     /pseudo
     CDS             20008..21989
                     /gene="arnA"
                     /locus_tag="KJL99_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
     gene            21986..22876
                     /locus_tag="KJL99_001697"
     CDS             21986..22876
                     /locus_tag="KJL99_001697"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HBD4436454.1"
                     /translation="MTKVGLRIDVDAFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAIPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            22876..24528
                     /gene="arnT"
                     /locus_tag="KJL99_001698"
     CDS             22876..24528
                     /gene="arnT"
                     /locus_tag="KJL99_001698"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HBD4436455.1"
                     /translation="MKSVRYLIGIFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLLAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVISPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIVRQERLVLIQYRPK"
     gene            24525..24860
                     /gene="arnE"
                     /locus_tag="KJL99_001699"
     CDS             24525..24860
                     /gene="arnE"
                     /locus_tag="KJL99_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HBD4436456.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMMLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            24860..25246
                     /gene="arnF"
                     /locus_tag="KJL99_001700"
     CDS             24860..25246
                     /gene="arnF"
                     /locus_tag="KJL99_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HBD4436457.1"
                     /translation="MCLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(25240..25512)
                     /gene="pmrD"
                     /locus_tag="KJL99_001701"
     CDS             complement(25240..25512)
                     /gene="pmrD"
                     /locus_tag="KJL99_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HBD4436458.1"
                     /translation="MQWNGWHKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKV
                     GDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(25617..26972)
                     /gene="menE"
                     /locus_tag="KJL99_001702"
     CDS             complement(25617..26972)
                     /gene="menE"
                     /locus_tag="KJL99_001702"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HBD4436459.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVSVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(26969..27931)
                     /gene="menC"
                     /locus_tag="KJL99_001703"
     CDS             complement(26969..27931)
                     /gene="menC"
                     /locus_tag="KJL99_001703"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HBD4436460.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQENWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVQEQLL"
     gene            complement(27931..28788)
                     /gene="menB"
                     /locus_tag="KJL99_001704"
     CDS             complement(27931..28788)
                     /gene="menB"
                     /locus_tag="KJL99_001704"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HBD4436461.1"
                     /translation="MIYPDEAMPYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(28803..29561)
                     /gene="menH"
                     /locus_tag="KJL99_001705"
     CDS             complement(28803..29561)
                     /gene="menH"
                     /locus_tag="KJL99_001705"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HBD4436462.1"
                     /translation="MILHAQAKHGKLGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLLGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(29558..31228)
                     /gene="menD"
                     /locus_tag="KJL99_001706"
     CDS             complement(29558..31228)
                     /gene="menD"
                     /locus_tag="KJL99_001706"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HBD4436463.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HHPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(31317..32612)
                     /gene="menF"
                     /locus_tag="KJL99_001707"
     CDS             complement(31317..32612)
                     /gene="menF"
                     /locus_tag="KJL99_001707"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HBD4436464.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(32691..32996)
                     /gene="elaB"
                     /locus_tag="KJL99_001708"
     CDS             complement(32691..32996)
                     /gene="elaB"
                     /locus_tag="KJL99_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HBD4436465.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKAHAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(33051..33512)
                     /locus_tag="KJL99_001709"
     CDS             complement(33051..33512)
                     /locus_tag="KJL99_001709"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBD4436466.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            33577..34494
                     /gene="rbn"
                     /locus_tag="KJL99_001710"
     CDS             33577..34494
                     /gene="rbn"
                     /locus_tag="KJL99_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HBD4436467.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            34685..34873
                     /locus_tag="KJL99_001711"
                     /pseudo
     CDS             34685..34873
                     /locus_tag="KJL99_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311184.2"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
BASE COUNT         8536 a         9339 c         8648 g         8380 t
ORIGIN      
        1 cgtaatgcct gaaaggcttt gcctgctggc gtgacaactc gctttgatat catatccctg
       61 gcataaatca atggccgccg cgacaccgtt agcagagaaa agagatgcaa tcattgacgg
      121 gagtagagat aaatactcct tgcgtagaaa gataatccgc atattaatct gaccatcatt
      181 ttatataaga cggcatattt aaacgcatta tacaatatgg gttatctttc ttttgtaaat
      241 tgtccaaccc gatgcaatgg ctattttgtt attatttgat gaataatatc agtgcgtcat
      301 aattcaagtt aataacctta agggatacca gttatattta aactaaatta aagtaatgaa
      361 taattttctt ataatataag gtaaattaac aaaatggctt cgcatttaac aataaccgaa
      421 tagaaaacaa ccatttcgac atcaacaatc gcttacattc gcttatatat tgaccacaac
      481 tgatacatca gattatgtga tgactcgtgc ttagatcaat ttttgcaatc attagcaaaa
      541 agattaataa accatctata tcaatttatc caacctattc tgccgttcaa gaaatcgccg
      601 aacagttatt tttaacaaat ttttctcttc ccattgactt tcccggacac cttgtctgac
      661 ctaaggtgcg cgaaagccac tttttccttc ctgagttatc cacaaagtta tgcacttgca
      721 agagggtcat tttcacacta tcttgcagtg aatcccaaac atacccccta tatatagtgt
      781 tctaagcagc ttcccttact acaggtagtc tgcatgaaac tattgcggaa agaattccaa
      841 aaacaggtac gacatacatg aatcagaatc tgctggtgac aaagcgcgac ggtagcacag
      901 agcgcatcaa tctcgacaaa atccatcgcg ttctggattg ggcggcagaa ggactgcata
      961 acgtttcgat ttcccaggtc gagctgcgct cccacattca gttttatgac ggtatcaaga
     1021 cctctgacat ccacgaaact attatcaagg ctgccgcaga cctgatctcc cgtgatgcgc
     1081 cggattatca gtatctcgcc gcgcgcctgg cgatcttcca cctgcgtaaa aaagcctacg
     1141 gccagtttga gccgcctgcg ctgtacgacc acgtggtgaa aatggtcgag atgggcaaat
     1201 acgataatca tctgctggaa gactacacgg aagaagagtt caagcagatg gacacgttta
     1261 tcgatcacga ccgtgatatg accttctctt atgctgccgt taagcagctg gaaggcaaat
     1321 atctagtaca gaaccgcgtg accggcgaaa tctacgaaag cgcccagttc ctttatattc
     1381 ttgttgccgc gtgcctgttc tcgaactacc cgcgtgaaac gcgcctgcaa tacgtgaagc
     1441 gtttttatga cgcggtttcc acatttaaaa tttcgctgcc gacgccaatc atgtccggcg
     1501 tgcgtacccc gactcgtcag ttcagctcct gcgtactgat cgagtgcggt gacagcctgg
     1561 attccatcaa cgccacctcc agcgcgattg tgaaatatgt ttcccagcgt gccgggatcg
     1621 gcatcaacgc cggacgtatt cgtgcgctgg gcagcccgat tcgcggtggc gaagcgttcc
     1681 ataccggctg tatcccgttc tacaaacatt tccagacagc ggtgaaatcc tgctctcagg
     1741 gcggtgtgcg cggcggtgcg gcaacgctgt tctacccgat gtggcatctg gaagtggaaa
     1801 gcctgctggt gttgaaaaac aaccgtgggg ttgaaggcaa ccgcgtgcgt catatggact
     1861 acggggtaca aatcaacaaa ctgatgtata cccgcctgct gaaaggtgaa gatatcaccc
     1921 tgttcagccc gtccgacgta ccggggctgt acgacgcgtt cttcgccgat caggaagagt
     1981 ttgaacgcct gtataccaaa tatgagaaag acgacagcat ccgcaagcag cgtgtgaaag
     2041 ccgttgagct gttctcgctg atgatgcagg aacgtgcgtc taccggtcgt atctatattc
     2101 agaacgttga ccactgtaat acccatagtc cgtttgatcc gaccatcgcg ccagttcgcc
     2161 agtctaacct gtgcctagaa attgccctgc cgaccaaacc gctgaacgac gtcaacgacg
     2221 agaacggtga aatcgcgctg tgtacgctgt ctgctttcaa cctgggcgca attaatagcc
     2281 tggatgaact ggaagagctg gcgattctgg cggttcgtgc acttgacgcg ctactggatt
     2341 atcaggatta cccgatcccg gccgccaaac gtggagcgat ggggcgtcgt acgctgggta
     2401 ttggtgtgat taacttcgct tactatctgg cgaagcacgg taaacgctac tccgacggca
     2461 gcgccaacaa cctgacgcat aaaaccttcg aagcgattca gtattacctg ctgaaagcgt
     2521 ctaatgagct ggctaaagag caaggcgcgt gcccgtggtt taacgaaacc acttacgcga
     2581 aagggatcct gccgatcgat acctataaga aagatctgga tgccatcgct aatgagccgc
     2641 tgcattacga ctgggaagca ttgcgtgaat caatcaaaac gcacggcctg cgtaactcca
     2701 cgctttctgc cctgatgccg tccgagacct cttcgcagat ctctaacgcc actaacggta
     2761 ttgaaccgcc gcgcggttac gtcagcatca aagcgtcgaa agacggtatt ttgcgccagg
     2821 tggtgccgga ctacgagcac ctgcacgacg cctatgagct gctgtgggaa atgccgggta
     2881 acgatggcta cctgcaactg gtaggtatca tgcagaaatt tatcgatcag tcgatctctg
     2941 ctaacaccaa ctacgatccg tcacgtttcc cgtcaggaaa agtgccgatg cagcagttgc
     3001 tgaaagacct gctcaccgcc tacaaattcg gggtcaaaac gctgtattat cagaacaccc
     3061 gtgacggcgc tgaagacgcg caggacgatc tggtaccgtc aatccaggac gatggctgcg
     3121 aaagcggcgc atgtaagatc tgatattgag atgccggatg cggcgtaaac gccttatccg
     3181 gcctacggct cggtttgtag gcctgataag acgcgccagc gtcgcatcag gctccgggtg
     3241 ccggatgcag cgtgaacgcc ttatccggcc tacggctcgg atttgtaggc ctgataagac
     3301 gcgccagcgt cgcatcaggc acaggatgcg gcgtaaaatg ccttatccgg cattaaactc
     3361 ccaacaggac acactcatgg catataccac cttttcacag acgaaaaatg atcagctcaa
     3421 agaaccgatg ttctttggtc agccggtcaa cgtggctcgc tacgatcagc aaaaatatga
     3481 catcttcgaa aagctgatcg aaaagcagct ctctttcttc tggcgtccgg aagaagttga
     3541 cgtctcccgc gaccgtatag attaccaggc gctgccggag cacgaaaaac acatctttat
     3601 cagcaacctg aaatatcaga cgctgctgga ttccattcag ggtcgtagcc cgaacgtggc
     3661 gctattgccg cttatttcta ttccggaact ggaaacctgg gtcgaaacct gggcgttctc
     3721 agaaacgatt cattcccgtt cctatactca tatcattcgt aatatcgtta acgatccgtc
     3781 tgttgtgttt gacgatatcg tcaccaacga gcagatccag aaacgtgcgg aagggatctc
     3841 cagctattac gatgaactga tcgaaatgac cagctactgg caccttctgg gcgaaggcac
     3901 ccacaccgtt aacggtaaaa ccgtaaccgt tagcctgcgc gagctgaaga aaaaactgta
     3961 tctctgcctg atgagcgtta acgcgctgga agcgatccgc ttctacgtca gctttgcttg
     4021 ttcctttgcc tttgcagaac gcgaactgat ggaaggtaac gccaagatta ttcgcctgat
     4081 tgcccgcgat gaagccctgc acctgaccgg tactcagcat atgctgaatc tgctgcgcag
     4141 cggcgcggat gatccggaga tggcggaaat tgccgaagaa tgtaagcagg agtgctatga
     4201 cctgtttgtc caggcggcac agcaagagaa agactgggcg gattatctgt tccgtgacgg
     4261 ttcgatgatt ggtctgaata aagatattct ctgccagtac gttgaataca tcaccaatat
     4321 ccgcatgcag gcagtcggtt tggatctgcc gttccagacg cgctccaacc cgatcccgtg
     4381 gatcaacacc tggctggtgt ctgataacgt gcaggttgct ccgcaggaag tggaagtcag
     4441 ttcttatctg gtcgggcaga ttgacgcgga agtggacacc gacgatttga gtaacttcca
     4501 gctctgatgg cccgcgttac cctgcgcatc actggcacac aactgctgtg ccaggatgaa
     4561 cacccttccc ttctggcggc gctggaatcc cacaatgtgg cggttgagta ccagtgccgc
     4621 gaaggttact gcggctcctg tcgcacccga ctggtcgcag gtcaggtgga ctggattgcc
     4681 gaaccgttag cctttattca gccgggggaa attttgccct gttgttgccg ggcaaaaggc
     4741 gatattgaaa tcgagttgtg atcgggtact agcgccagat acgacgctta cgcgtattat
     4801 ctggcctaca acgattacat ggcgtagtaa tacgctttca cctgctccca gtccgcttta
     4861 gggatcggct ccagatattt ttccagctgg cggaagtcat gattaatcgc tttatcgcga
     4921 cgcaagcggc gacgactttt ctccagatca agaaaaccag cttctgcctt accttctgtt
     4981 ttcacataga tatggcgaac atagcaacag ccatgctgac gattaacgct atgcattttc
     5041 ttaaacgcca gtgccactgc tttcaacatg gcttgccgta cttcgtcaga ataaggcgat
     5101 actgcatgcc gggcatacca gtcagcaatg ctgatgaacc ccgccatatc ttcagtcacc
     5161 agcaacgcgc gccattcacc ctcaattttc accgcttcac caaaaacgat cttcggcacg
     5221 atgacaccgg cccgttccag ttctttaatt accgcaacct cacggacaat cgttggtcgg
     5281 ccgaacggat aacgtactga atgaaacaga tgatgcgtca tgcgctttac atacagcttt
     5341 ttgccgttgc gctcgacgca ttgcaccccg ctcataccat tacggcgata gttaggctct
     5401 tcaacccagt cgccctctgt tgcccaccag tgattaaatt cgtcgtactt tgctgaaact
     5461 gccataccct atcgcctgtc atttttatta acgacaatga ctataggtgg ttacctgagg
     5521 aaaatcttaa tgaaacgtgt cgtattaatg aatttctttt gccgctacag gtgtgacacc
     5581 gcttttgccc ttcatttgca gcgcatagat tccaccagcc actaccacta atagtgcgcc
     5641 cagcagataa aatgtgaatt taatcagtcg gatacagcga tgaattttat tcgacaggga
     5701 ttaggcatcg ccttacaacc agagttaacg ctgaaaagca ttgcaggtga attgtgttcc
     5761 gttcctcacg aaccaacctt ctatcgacag atttcgttgc tgactaaaga aaagccggta
     5821 gaaggcagtc cactgttttt actacaaatg tgcatggaac aattagtggc gattggaaaa
     5881 atttgatatg agacaggatg gcgcatgaac gccatcctgc agtaacatta ctctttatta
     5941 agaaatttta ctgccttatc agggaaatca gtaaacagcc catttacacc cgctttgtta
     6001 tacagagcat catataactg attcacatca ggagtgtatt caggcagttt atctgaccgc
     6061 acggtataag gatgcactac cagtttattc tgctgggcat cttgcaccat gccagtgagt
     6121 ttgatattac ccggctgcga tgtctcctca atcaacatat ggtaatccgg accaatacca
     6181 tctgcatatt ccgccacctg tttcatggct cccggcttaa acatccagtc gtagttgtaa
     6241 ttaacccagc ttccatccgg ctgtttctgc tgcgtttcat tccagtcggt ataggcaatc
     6301 agctgtacca gattgagctc catgcccatt ttgggttcca gctcattctt aatacgcttc
     6361 agctcatcag catcaaaaca ttgcaaataa actttatcgt ctttaccggt gtaaccatat
     6421 ttcttcagca cttccagcgt ttttgccgca atatccttcc cttcctgatg atggaaccac
     6481 ggcgctttga tttctggata gataccgata tttttcccgg tagagtgatt taacccctga
     6541 acaaattcaa tctcttcttc aaaggtgtgc acccggaagt cggacttacc cattgggaaa
     6601 cgccccggat aagtctgtac ttttttaccg ttttcaatat cgaaaccttc ggtaaatttc
     6661 agcgacttaa tttcatccag cgtgaaatct atcgcgtagt aacgaccgtc tttgcgcgcc
     6721 cgatccggga aacgatcggc aacatcagta acacgatcga gataatggtc atgcagaaca
     6781 accagatggt cgtctttggt catcaccaaa tcctgttcca gataatccgc tccctgcgca
     6841 tacgccatcg cttttgctgg cagcgtatgc tccggcaaat atccactggc accgcgatgg
     6901 gcgatgacta ttttttcgtt gctgtccgcc gccattgcac tgcttcccat gactatagtg
     6961 ctcatcatga tcgccatgct aaggtttttc agcttcaatt tcatgccatt agcctccgtt
     7021 gcgtttttgc agtaattgtt catggcgacg tttttcgcca atcatcacaa caatcaacaa
     7081 gataaccgcc agaatgctgc cgccaatcat taccataaag ccgccatccc agccgaagaa
     7141 gtccacggtg tagccaacaa tcgcgctcgc cgccaccgaa ccgcccaggt aaccaaacag
     7201 cccggtaaag cccgctgccg tacctgccgc ttttttcggt gccagttcca gcgcatgcag
     7261 accgatcagc atcacaggac cgtagatcag gaagccgata acaatcatac aaatcatatc
     7321 gacggttggg ttacctgccg ggttcatcca gtaaacgata gtcgcgatgg tcaccagtgt
     7381 cataaagaag acgccggttg ccccacggtt accacggaag actttgtccg acatccagcc
     7441 gcacagcagg gtgcccggaa tacccgcata ctcatagaag aagtaggccc aggaggattt
     7501 atccagcgcg aaatgcttaa cctctttcag ataagtcggt gaccagtcga ggatgccgta
     7561 acgcagcaga taaacgaaca cgttggcgat ggcgatgtac cacagcagtt tgttcggcag
     7621 cacgtactgc atgaagattt gcttcgcggt cagctcctgt tccgcttttt cgttatagtc
     7681 gtccggataa tcatttttgt actcttcgat cggcggcaag ccacaggatt gcggggtatc
     7741 gcgcatcatc gcaaaggcga ataatgccac cagaatggcg cagaaagcag gcatatagag
     7801 cgccgcatgc cagtcattga accaggccat ccccagcagg aacagcagcg gcggaatacc
     7861 accaccgacg ttgtgcgcac agttccacac tgacacaatg ccgccacgtt ctttctgcga
     7921 ccaccagtgc accatagtac gaccacacgg cggccacccc atcccctgga accaaccgca
     7981 gaggaacaac agtacaaaca tcaccgcaat gctcgacgtc gcccatggca caaagcccat
     8041 aaacaacatc actgccgccg ccagaatcaa acctgcgggc aggaaaacgc gcggattcga
     8101 gcgatccgat accgaaccca tgatgaattt cgaaaatcca taagcaatcg agatccccga
     8161 aagggcaaaa cctaaatcac cgcgtgagaa tccctgctca accagataag gcatagcaag
     8221 cgcaaagttc ttacgaacca aatagtaagc cgcatagcca aagaatatcc ccaggaaaat
     8281 ttgccagcgc aatcgacgat aagtcggatc gatctccgcg gcaggtaagc gcgctttgtg
     8341 tggcgctggt ttaaaaatac tcaacattga tagcctccgt ggcccgtggt cttatttatg
     8401 attaacagcc tgattcagtg agagaacctg ccgtttcttg agttgccgcg atgttaagaa
     8461 aacattcata aattaaatgt gaattgccgc acacattatt aaataagatt tacaaaatgt
     8521 tcaaaatgac gcatgaaatc acgttttact ttcgaatcat gagcgaatat gcgcgaaatc
     8581 aaacaattca tgtttttact atggctaaat ggtaaaaaac gaacttcaga gggataacaa
     8641 tgaaaactcg cgactcgcaa tcaagtgacg tgattatcat tggcggcggc gcaacgggag
     8701 ccgggattgc ccgcgactgt gccctgcgcg ggctgcgcgt gattttggtt gagcgccacg
     8761 acatcgcaac cggtgccacc gggcgtaacc acggcctgct gcacagcggt gcgcgctatg
     8821 cagtaaccga tgcggaatcg gcccgcgaat gcattagtga aaaccagatc ctgaaacgca
     8881 ttgcacgtca ctgcgttgaa ccaaccaacg gcctgtttat caccctgccg gaagatgacc
     8941 tctcattcca ggccactttt attcgcgcct gcgaagaagc agggatcagc gcagaagcta
     9001 tagacccgca gcaagcgcgc attatcgaac ctgccgttaa cccggcactg attggcgcgg
     9061 tgaaagttcc ggatggcacc gttgatccat ttcgtctgac cgcagcaaac atgctggatg
     9121 ccaaagaaca cggtgccgtt atccttaccg ctcatgaagt cacggggctg attcgtgaag
     9181 gcgcgacggt gtgcggtgtt cgtgtacgta accatctcac cggcgaaact caggcccttc
     9241 atgcacctgt cgtggttaat gccgctggga tctgggggca acacattgcc gaatatgccg
     9301 atctgcgcat tcgcatgttc ccggcgaaag gatcgctgct gatcatggat caccgcatta
     9361 accagcatgt gatcaaccgc tgccgtaaac cttccgacgc cgatattctg gtgcctggcg
     9421 ataccatttc gctgattggt accacctctt tacgtattga ttacaacgag attgacgata
     9481 atcgagtgac ggcagaagag gttgatattc tgctgcgtga aggggaaaaa ctggcccccg
     9541 tgatggcgaa aacgcgcatt ttgcgggcct attctggcgt gcgcccgctg gttgccagcg
     9601 atgacgaccc gagcggacgt aacgtcagcc gtggcatcgt gctgctcgac catgctgaac
     9661 gcgatggtct ggacggattt atcaccatca ccggtggcaa actgatgacc tatcggctga
     9721 tggctgaatg ggctaccgac gcggtatgcc gcaaactggg caacacgcgc ccctgtacga
     9781 ctgccgatct ggcactgcct ggttcacaag aacccgctga agttaccttg cgtaaagtca
     9841 tctccctgcc tgccccgctg cgcggttctg cggtttatcg tcatggcgat cgcacgcctg
     9901 cctggctgag cgaaggccgt ctacaccgta gcctggtatg tgagtgcgaa gcggtaactg
     9961 cgggtgaagt gcagtacgcg gtagaaaatt taaacgttaa tagcctgctg gatttacgcc
    10021 gtcgtacccg tgtggggatg ggcacctgcc agggcgaact ctgcgcctgc cgcgctgccg
    10081 gactgctgca acggtttaac gtcacgacgt ccgcgcaatc tatcgagcaa ctttccacct
    10141 tccttaacga acgctggaaa ggcgtgcaac ccatcgcctg gggagacgca ctgcgcgaaa
    10201 gcgaatttac ccgctgggtt tatcagggac tgtgtggtct ggagaaggag cagaaagatg
    10261 cgctttgata ctgtcattat gggcggcggc ctcgccggat tactctgtgg cctgcaactg
    10321 caaaaacacg gcctgcgctg tgccattgtc acgcgtggtc aaagcgcgtt gcatttctca
    10381 tccggttcgc tggatttgct gagccatctg ccagatggtc aacccgtggc agacattcac
    10441 agtggactgg aatctttgcg tcagcaggca cctgcacatc cttactctct tctcgggcca
    10501 caacgcgtgc tcgatctcgc ttgccaggcg caggcattaa tcgctgaaag cggtgcgcaa
    10561 ttgcagggca gcgtagaact ggctcaccag cggattacgc cgctcggcac tttgcgtgca
    10621 acctggttaa gttcgccaga agttcccgtc tggccgctac ccgcgaagaa gatatgtgta
    10681 gtggggataa gcgggctgat ggatttccag gcgcatcttg cggcggcttc gttgcgcgaa
    10741 ctggatcttt cggttgaaac cgcagaaata gagctgccgg aactggatgt cctgcgcaat
    10801 aacgccaccg aatttcgcgc ggtgaatatc gcccgtttcc ttgataatga agaaaactgg
    10861 ccgctgttac ttgatgcgct tattcctgtc gccaatacct gcgaaatgat cctgatgccc
    10921 gcctgcttcg gcctggccga tgacaaactg tggcgttggt tgaatgaaaa actaccttgt
    10981 tcactgatgc ttttgccaac gctgccgcct tccgtgctgg gcattcgtct gcaaaaccag
    11041 ttacagcgcc agtttgtgcg ccaaggtgga gtgtggatgc cgggcgatga agtgaaaaaa
    11101 gtgacctgta aaaatggcgt agtgaatgaa atctggaccc gcaatcacgc cgatattccg
    11161 ctacgtccac gtttcgcggt tctcgccagc ggcagtttct ttagtggcgg actagtagcg
    11221 gaacgtaacg gcattcgcga gccgattctc ggccttgatg tgctacaaac cgccacgcgg
    11281 ggtgaatggt ataagggcga tttttttgcg ccgcaaccgt ggcagcagtt cggtgtaacc
    11341 actgatgaga cgctacgccc gtcgcaggca gggcaaacca ttgagaacct gtttgccatc
    11401 ggttcggtgc tgggcggatt tgatcccatc gcccagggat gcggcggcgg tgtttgtgcc
    11461 gtcagtgctt tacatgccgc tcaacagatt gcccaacgcg caggaggcca acaatgaacg
    11521 acaccagctt cgaaaactgc attaagtgca ccgtctgcac caccgcctgc ccggtgagcc
    11581 gggtgaatcc cggttatcca gggccaaaac aagccgggcc ggatggcgag cgtctgcgtt
    11641 tgaaagatgg cgcactgtat gacgaggcgc tgaaatattg catcaactgc aaacgttgtg
    11701 aagtcgcctg cccgtccgat gtgaagattg gcgatattat ccagcgcgcg cgggcgaaat
    11761 atgacaccac gcgcccgtcg ctgcgtaatt ttgtgttgag tcataccgac ctgatgggta
    11821 gcgtttccac gccgttcgca ccaatcgtca acaccgctac ctcgctgaaa ccggtgcggc
    11881 agctgcttga tgcggcgtta aaaatcgatc atcgtcgcac gctaccgaaa tactccttcg
    11941 gcacgttccg tcgctggtat cgcagcatag cggctcagca agcacaatat aaagaccagg
    12001 tcgctttctt tcacggctgc ttcgttaact acaaccatcc gcagttaggt aaagatttaa
    12061 ttaaagtgct caacgcaatg ggtaccggtg tacaactgct cagcaaagaa aaatgctgcg
    12121 gcgtaccgct aatcgccaac ggctttaccg ataaagcacg caaacaggca attacgaatg
    12181 tagagtcgat ccgcgaagct gtgggagtaa aaggcattcc ggtgattgcc acctcctcaa
    12241 cctgtacatt tgccctgcgc gacgaatacc cggaagtgct gaatgtcgac aacaaaggct
    12301 tgcgcgatca tatcgaactg gcaacccgct ggctgtggcg caagctggac gaaggcaaaa
    12361 cgttaccgct gaaaccgctg ccgctgaaag tggtttatca cactccgtgc catatggaaa
    12421 aaatgggctg gacgctctac accctggagc tgttgcgtaa catcccgggg cttgagttaa
    12481 cggtgctgga ttcccagtgc tgcggtattg cgggtactta cggtttcaaa aaagagaact
    12541 accccacctc acaagccatc ggcgcaccac tgttccgcca gatagaagaa agcggcgcag
    12601 atctggtggt caccgactgc gaaacctgta aatggcagat tgagatgtcc acaagtcttc
    12661 gctgcgaaca tccgattacg ctactggccc aggcgctggc ttaaactccc ttctgatgcc
    12721 cggtaagcat gtggttaccg ggcatttttg cgtacacgat tccgtgccca atgtatgcgt
    12781 tgcaacgcag tgaaaattcc tctgaaaacg tctcgcaaag gctgaaactg gcagatgtca
    12841 aaggcctggg ataaccgtaa tgtcgcgtca tcataaatat caggtgacgg acaaccatga
    12901 ccgaatcaac aacctcctcc ccgcatgatg cggtatttaa aacctttata ttcacacccg
    12961 aaaccgcacg ggattttctc gaaatacatt taccagaacc actgcgcaag ctttgcaacc
    13021 tgcaaacctt acgcctggaa cccactagtt ttattgaaaa aagtttacgc gcttactact
    13081 cggatgtttt gtggtccgtg gaaaccagcg acggtgacgg ctatatctac tgcgtgattg
    13141 aacatcaaag ctctgcagaa aagaatatgg cttttcggcc aatgcgctat gccactgccg
    13201 ccatgcagag tcacctagat aaaggctatg acagagttcc gctggtggtg ccattgctgt
    13261 tttatcatgg cgaaacatcg ccctacccgt actcacttaa ctggctggat gagtttgacg
    13321 atccgcaact tgcccggcag ttgtacaccg aagcttttcc gttggtggat atcaccatcg
    13381 tacctgacga tgagatcatg caacatcggc gtatagctct gctggaactg attcaaaagc
    13441 atattcgcga ccacgattta atcggcatgg tcgacaggat caccacgctt ttggttagag
    13501 gcttcactaa tgacagccag ctacaaacac tgtttaatta tctgctgcaa tgcggcgata
    13561 cctcccgttt cacccgtttt attcaggaga ttgccgaacg ttcaccacta caaaaggaga
    13621 gattaatgac tattgctgaa cggctacggc aggaaggaca tcaaattggc tggcaagaag
    13681 gtaaattagt aggtttgcag caaggtaaat tagaaggttt gcaggaaggt atgcatgaac
    13741 aagccattaa aattgctttg cgcatgctgg aacagggcat tgatcgtgac caagtgctcg
    13801 cggccaccca gctaagcgaa gccgatctgg cagcgaataa ccactaatta acacaggccc
    13861 acagccgatc cccatgggcc tttgatatca ataactacct tttatgcgtg gcccattttt
    13921 gcctgattta aacatcgcca gtcgttgatc cagggcatcg ctgtagagca tcgtgtcaac
    13981 gccaacagcg acaaagttcg ctccccacgc caggcattgc tgcgccatat caggagccac
    14041 agccagaaaa ccagccgctt tacccgcagc acggatccgc cgaatgctgg tttcaataat
    14101 tcgctgcact tccgggtgcc cggcgttatc cgggtagccc aacgacgcag aaagatccgc
    14161 tggtccaata aacacgccat caatcccttc gacgtcgagg atctcgtcca ggttatccag
    14221 tgccgtttta ctttccacct gcactaacag acaaagcgaa tcgttgactt gcgccatgta
    14281 attctcaatg cgtccccagc gcgcagcccg tgccacactg gccccaacac cacgctcacc
    14341 gtagggaggg ttggacaaat tttgccggag ggggaatgtg ccggtgcagt aaaccggcac
    14401 agagtggcga aatcagacgg ataaagtcat ggactcaaca acctcaatcc agccatgctc
    14461 actagcgatg tcctggccgt ttaaccaacg gcgtagcata ttcagtgcca tcattgcgca
    14521 cacttcctga cggatagcca ggctgtagcg tgtagtgctg aaacgcacac gcagagcgaa
    14581 agtgccgtct ggcgtggcta gcgcaaagtt gagatgctcg ttctcgaaac ccgaaacagc
    14641 cagtgccagc ccggcaaaat ggttggcccg ccgttctgta atccagtgcg cagtttgcgc
    14701 cagggtttct tcctgtgaag gaatcacttc acacgccagc aatggagcac ctgcgcgaga
    14761 aagttgcaaa gccaataaac caccggtgaa ttgctcgctc aacgtcaggc tgaactggcg
    14821 gttttgcaat tcgcgactga tctgcgcggg cagtccttcg gtgccttcga aaatcacgct
    14881 ctgtccggca acacgtttaa cgtccagcca cagtttttcc atcgcctgtt gctcgcttgc
    14941 cggtccggtg agtttcagtt cgatgatcgg cattgaggag cgatagccca ttgttacgcc
    15001 cggcggcagt tgtagagtgt ccaggctttg tgccagatcg ctttccgaac gaccaaaggt
    15061 agttaaacgc agacaaaccg gcggctgtgg taaagaaaaa cgctcgcgca ggcgcggcag
    15121 gatttcgtgc tcgaccatca ccttaaattc tgacggtacg ccgggagtaa agaacatcag
    15181 gcaacgatta agctgcacgg caaaaccaca ggcggtgcct accgggttat tgataaattc
    15241 agcactggca ggcagctccg cttgtttacg gttgctcggt gccattactc gtccacgttc
    15301 gtggaaatag cgttccatct ctttgagcca ggcttcatgc agcaccaggc cttcaccttt
    15361 tgctgtcgca gcggcgagtg cgcttaaatc atcgctggtc ggtcccagcc cgccgttaac
    15421 gatcagcaca tcggcgtgct gactacgttc gcgaagaatg gtgactaagt catcaaggtt
    15481 atcccccacc gtattgcggc gagataatgg cagcccctga tgaaagaaaa aatcggccag
    15541 ccaggcagcg ttagtgtcaa cgatttgccc gtataacact tcatccccgg tggatagcat
    15601 ttccactttt aacatggtta tctcctgctt ttgttatcga atcactataa cgcaaaggga
    15661 gataaggcgt agtaagaaac tgaatgccgc gccggaaggg atcagaaact ggcgttaacc
    15721 ccaacataca ggccgtcagc cacagcgttg tcgcggttac cgtctttacc cgacagattc
    15781 aggtagcgat aacccgcctc aatactgact ggacgcataa tggtgtaacg cgcgccagca
    15841 ttcgcttcct cataactttg aataccgctg gagagcgaat ccggagagta gtaatactcg
    15901 ccaaacaaac ggaagctgtt gccaattttc cactgcaaac cacctcctac cgctgcggca
    15961 taaccttcat cgccgtaatt cgggttggtg tacacgcctt ttccgccaac ggtcgccatt
    16021 aacggcccga gaggcagatt caaccccagc cccacgcccg cgacgtcacc gtcgtcgtcg
    16081 ttatgtgtcc agttaccgct taaagccagg cccgtcgatt cagtcccaaa accgacacca
    16141 atattggtgt attctttacc cgcctggccg ctgatgctca ttgcattgac cgatgcagaa
    16201 accaacagca ttccggccag acctgctagc gcaatttttt tcattatccc ctttcccaca
    16261 agcaattaat caaaattccc aaagctgcgg gattgtactg cctggcacag aggattcaat
    16321 gagcgaaaaa agggatctag tcacttattg taactaaata cttcttaaat aattaaacgc
    16381 cgaaaaaata aacacacttt accgatacac acaaaaataa taataagtgc agttatcatt
    16441 gcaaaagaga ttactgtcac tttcacatat ttctgagtta ccgtattctc atggtttctt
    16501 tttatttata aggaataccc ctgtgcgaca acggactatt gtatgccctt tgattcaaaa
    16561 tgatggtgct tatttgctgt gcaaaatggc cgacgatcgc ggcgttttcc ccggtcaatg
    16621 ggcgctttcg ggtggcggcg tggagtctgg cgaacgaatt gaagaggcac tacgccgcga
    16681 aattcgcgaa gaactgggag aacagctgct tttgacagaa atcacgccgt ggaccttcag
    16741 cgatgatatt cgcaccaaga catatgcaga tggtcgcaag gaagagattt atatgattta
    16801 cctgattttt gactgcgttt ctgccaaccg agaagtgaaa ataaacgaag agtttcagga
    16861 ctacgcgtgg gtaaaacctg aagatctggt gcattatgat ttaaatgttg ccacccgaaa
    16921 aacgttacgt ttgaaaggtc ttctttaaca atgatgacag ccatcactgg ctgtcagatt
    16981 gaaaatttag tggttaacga attccccatc cagataaact ttgccttttt ccacatgcat
    17041 gactaaacca tccagataat caggtttaaa tatcgcatca cgcttatttt tagcaatata
    17101 tgtcaggcaa tgattatggg tgaaaataac gatattttta tcaggcgctt tgttttgtaa
    17161 gtccttaatt gcactataaa tctcattacc gcactgaaga agtcgtttat ctaccgtcag
    17221 ctttttaccc gcggaaaacc aggtcgccga ctgaatggtc cggacggtat tactggaata
    17281 aagatcgaaa tcagagatgt cagcgctaaa agcgttgccc agttcacggg catcctgggt
    17341 acctttaacc gtaatacctg ttttatctga caagcattga ttggttgaac gatcgcaacg
    17401 ttcagcatgg cgaaacaaaa cgacaaccgg gtgctgctgt gccagtctgg ccaatgtttt
    17461 attgtcgata cgtggcaaac cattgctact ccaggcggca tgagtaccca gtccggcaat
    17521 tgcagcgagc gccagtaaaa tgatgaaata tttttttgac ttcaatgatg agcggcaaaa
    17581 agctaacacg taaactccac ctatagacaa gcgcaaccag acaattaccg tgaaattgag
    17641 cgacatttct ggcgataatt agcagttggt gtaatattaa aaatcctacg atgtcggcaa
    17701 aatgcctcaa atttttgcca aatgcaaagc ctaaataaga aaaaatataa aaatttcaat
    17761 atttacgtct aatattagtt tcttaaggtt aagttaatat tctatcctta aaatttcgct
    17821 ccaaatggca aaatatacac aacactcttt atagcaaata taagtggaca ggtattcaat
    17881 ggcggaagga aaagcaatgt cagaattttt gcctttttcg cgaccagcaa tgggcgtgga
    17941 ggaactcgct gcagttaaag aggttctcga atccggttgg atcacaaccg gtccgaagaa
    18001 tcaggcgctt gagcaagcct tttgccagtt gacgggaaat cagcatgcca tcgcggtcag
    18061 ttcagccacc gccggaatgc atatcacgct aatggcgttg gaaattggca agggcgatga
    18121 agtgattacg ccttccctga cctgggtttc aaccctcaat atgatttcct tgctgggtgc
    18181 aacgccggta atggtggatg tcgaccgcga tacgctgatg gtcacgcctg aagcgatcga
    18241 atcagccatt acgccacgca ctaaagccat cattccggtg cattatgccg gtgcgccagc
    18301 agatattgac gccattcgcg ccattggcga acgttacggc atcgcagtta tcgaagatgc
    18361 tgcccatgcc gtcggtacgt attacaaagg gcgacatatt ggcgcaaaag gtaccgctat
    18421 tttttcattt catgccatta aaaatattac ctgtgctgaa ggtggcctga ttgtaactga
    18481 taatgaaaac cttgcccgcc agctacggat gctgaaattt cacggtctgg gtgtcgatgc
    18541 ctatgacaga caaacctggg gccgtgcacc gcaggctgaa gtcttaacac cgggctataa
    18601 gtacaatctg accgatatta acgccgcgat tgccctgaca cagttagtca aattagagca
    18661 cctcaacacc cgtcggcgcg aaattgccca gcaatatcag caagcactgg cagctctccc
    18721 ctttcagcca ttaagccttc ccgcctggcc gcacgttcac gcctggcatc tgtttattat
    18781 tcgtgtcgat gaacaacgtt gtggtatcag tcgcgatgcg ttgatggaag cgttaaaaga
    18841 aagaggcatt ggtaccgggt tacatttccg cgccgctcac acacaaaaat attatcgcga
    18901 gcgttttccc acgctgtcgt taccgaatac cgaatggaat agcgaacgca tctgttcgtt
    18961 gccgctgttc ccggatatga ctaccgccga tgccgaccgc gtcatcacag cccttcagca
    19021 actcgcagga caataagcca tgtttgaaat ccaccctgtt aagaaagtct cggtggttat
    19081 tcccgtttat aacgagcagg aaagcttacc ggaattaatc aggcgcacca ccacagcctg
    19141 tgaatcgttg gggaaagagt atgagatcct gctgattgat gacggcagta gcgataattc
    19201 cgcgcatatg ctggtcgaag cctcacaagc ggagaacagc catattgtgt ctattttgat
    19261 taaccgcaat tacgggcaac attcagcgat tatggcggga ttcagtcacg ttactggcga
    19321 cttaattatt acccttgatg ccgatctcca gaatccgcca gaagaaatcc cccgcctggt
    19381 ggcaaaagcc gatgaaggtt acgacgtggt agggactgta cgccagaacc gccaggacag
    19441 ctggtttcgt aaaacagctt cgaagatgat taaccggctt attcagcgca ccactggcaa
    19501 agcgatgggt gactacggtt gtatgctgcg cgcctatcgc cgtcatattg tcgatgcgat
    19561 gttgcactgc catgaacgca gcacctttat cccgattctg gcgaatatct tcgcccgccg
    19621 tgcaattgaa attccagtac atcatgccga gcgtgagttt ggtgaatcca aatacagttt
    19681 tatgcgcctg attaatttga tgtacgacct ggtgacctgc cttaccacaa cgccgctacg
    19741 tatgctgagt ctgctcggca gcattattgc gattggaggt tttagcattg cggtgctgct
    19801 ggtgatttta cgcctgacct tcggaccaca atgggcggca gaaggcgtct ttatgctatt
    19861 tgccgtgctg tttactttta ttggcgctca gtttatcggc atgggattac tcggtgaata
    19921 tatcggcagg atctacaccg atgtccgcgc ccgcccccgc tattttgttc agcaagttat
    19981 ccgtccatcc agcaaggaaa atgaataatg aaaaccgtcg tttttgccta ccacgatatg
    20041 ggatgcctcg gtattgaagc tctgctggct gccggttacg aaattagcgc catttttacc
    20101 cataccgata atcccggtga aaaagccttt tatggttcgg tggctcatct ggcggcggaa
    20161 agagacattc cggtttatgc gccggataac gttaatcatc cgctgtgggt ggaacgcatt
    20221 gcccaactgt caccagaggt gattttctct ttttattatc gccatcttat ttgcgacgag
    20281 atttttcagc tcgctcccgc aggtgcattt aatctgcacg gttcgctgtt accaaaatat
    20341 cgtggtcgcg cgccgctgaa ctgggtgctg gtgaacggtg aaacggaaac gggcgttaca
    20401 ttgcaccgaa tggtaaacgt gccgatgctg gggccattgt ggcgcaactg cgcgttgcca
    20461 ttgcgccaga cgatatcgct attacgctgc atcataaatt gtgccatgcc gcgcgccagc
    20521 tactggaaca gacattaccc gccattaaac acggtaatat tctggaaatc gcccagcgcg
    20581 aaaacgaagc cacctgtttt ggtcgcagaa cgccggatga cagtttcctt gaatggcata
    20641 aaccggcatc cgtactgcac aacatggtac gtgccgttgc cgatccgtgg ccgggtgcct
    20701 tcagctatgt tggcaatcag aaattcaccg tctggtcgtc gcgtgttcat cctcatgcca
    20761 gcaaagcaca gccggggagc gtgatttctg ttgcgccact gctgattgcc tgtggcgatg
    20821 gcgcgctgga aatcgtcacc ggacaggcgg gcgacggcat tactatgcag ggctcgcaat
    20881 tagcgcagat gctgggcctg gtgcaaggtt cacgcttgaa tagccagcct gcctgcaccg
    20941 cccgacgccg tacccgggta ctcatcctcg gggtgaatgg ctttattggc aaccatctga
    21001 cagaacgcct gctgcgcgaa gatcattatg aagtttacgg tctggatatt ggcagcgatg
    21061 cgataagccg ttttctgaat catccacatt ttcactttgt cgaaggcgat atcagtattc
    21121 attccgaatg gattgagtat cacgtcaaaa aatgtgatgt cgtcttgccg ctggtggcga
    21181 tagccacgcc gattgaatat acccgcaacc cgctgcgcgt atttgaactc gattttgaag
    21241 agaatctgcg cattatccgc tactgcgtta agtaccgtaa gcgaatcatc ttcccgtcaa
    21301 cttcagaagt ttatgggatg tgtagcgata aatacttcga tgaggaccat tctaatttaa
    21361 tcgtcggccc ggtgaataaa ccacgctgga tttattcggt gtcaaaacaa ttacttgatc
    21421 gggtgatctg ggcctatggc gaaaaagagg gtttacagtt caccctcttc ctcccgttta
    21481 actggatggg accacgactg gataacctta atgcagcgcg aattggcagc tcccgcgcta
    21541 ttacgcaact cattctcaat ctggtagaag gttcaccaat taagctaatt gatggcggaa
    21601 aacaaaaacg ctgctttact gatatccggg acggtatcga ggcgttgtac cacattatcg
    21661 aaaatgcggg aaatcgctgc gacggcgaaa ttatcaacat tggcaatcct gagaacgaag
    21721 cgagcattga ggaactgggc gagatgctgc tggcgagctt cgaaaaacat ccgctgcgcc
    21781 atcatttccc accgtttgcg ggctttcgcg ttgtcgaaag tagctgctac tacggcaaag
    21841 gatatcagga cgtagagcat cgtaaaccga gcatccgcaa tgcccaccgc tgcctggact
    21901 gggagccgaa aattgatatg caggaaacca tcgacgaaac gctggatttc ttcctgcgca
    21961 ccgttgatct tacggataaa ccatcatgac caaagtaggc ttacgcattg atgtcgatgc
    22021 ctttcgtggc acccgtgaag gcgtgccgcg tctgctggaa atcttgagta agcataatat
    22081 tcaggccagc atttttttca gcgtcggccc ggacaatatg ggccgccatc tctggcgact
    22141 ggtgaagcca cagtttttgt ggaagatgct gcgctcaaac gcggcatcgc tttatggctg
    22201 ggatatttta ctggcaggta cggcctggcc aggtaaagag attggtcatg ccaatgccga
    22261 tatcattcgt gaagcggcta aacatcacga agtcggtctg cacgcctggg atcaccatgc
    22321 ctggcaagcc cgtagcggta actgggatcg gcaaacaatg atcgacgata ttgcacgcgg
    22381 tcttcgcact ctggaagaga ttatcggtca accggtaacc tgttctgccg ctgcgggctg
    22441 gcgtgccgac cagaaggtga tcgaagcaaa agaagcgttc catttgcgct acaacagcga
    22501 ttgtcgtggg gccataccgt tccgtccact gcttgaatca ggaaaccctg gcactgcgca
    22561 aattccggtg accttaccca cctgggatga agtgattggt cgggatgtga aagcagaaga
    22621 ttttaacggt tggttactca accgcatcct gcgagataaa ggcacgccgg tttataccat
    22681 tcatgcagaa gttgaaggct gcgcttatca gcataatttt gtggatctcc tcaaacgcgc
    22741 agctcaggaa ggcgtgacat tttgcccttt aagcgaactg ttatcagaga cgttgccgct
    22801 cggacaagtt gttcgcggaa atattgccgg acgtgaaggc tggctgggtt gccaacaaat
    22861 tgcgggtagt cgctgatgaa atcggtacgt taccttatcg gcatcttcgc gtttattgcc
    22921 tgctattacc tgttaccgat cagcacgcgt ctgctctggc aacccgatga aacgcgttat
    22981 gcggaaatca gtcgagaaat gctggcatcc ggcgactgga ttgtgcccca tctgttaggg
    23041 ctacgttatt tcgaaaaacc cattgccgga tactggatta acagcattgg gcaatggcta
    23101 tttggcgcga ataactttgg tgtgcgggca ggcgttatct ttgcgaccct gttaactgcc
    23161 gcgctggtga cctggtttac tctgcgctta tggcgcgata aacgtctggc tctactcgcc
    23221 acagtaattt atctctcatt gtttattgtc tatgccatcg gcacttatgc cgtgctcgat
    23281 ccgtttattg cattctggct ggtggcggga atgtgcagct tctggctggc aatgcaggca
    23341 cagacgtgga aaggcaaaag cgcaggattt ttactgctgg gaatcacctg cggcatgggg
    23401 gtgatgacca aaggttttct cgcccttgcc gtgccggtat taagcgtgct gccatgggta
    23461 gcgacgcaaa aacgctggaa agatctcttt atttacggct ggctggcggt tatcagttgc
    23521 gtactgacgg ttctcccctg gggactggcg atagcgcagc gggagcctga cttctggcac
    23581 tattttttct gggttgagca tattcaacgc tttgcactag atgatgccca acatagagct
    23641 ccgttctggt actacgtgcc ggtcatcatt gccggtagcc tgccgtggct gggattactc
    23701 cccggtgcac tgtacacagg ctggaaaaac cgcaagcatt ccgcaaccgt ctatttgttg
    23761 agctggacga taatgccgct gctgtttttc tccgtcgcta aaggcaaatt gcccacctat
    23821 attctttctt gctttgcacc tctggcaatg ctgttggcgc attacgcgtt gctggcagca
    23881 aaaaataatc ctctggcgct gcggattaat ggctggatta acatcgcttt tggcgtcact
    23941 ggcattattg ccacatttgt gatctccccc tggggaccaa tgaacacgcc ggtgtggcaa
    24001 accttcgaga gctataaagt cttttgtgcc tggtcgattt tttcgctatg ggcatttttc
    24061 ggctggtaca ccttaacaaa cgtcgaaaag acctggtctt tcgccgcgct ttgcccgctg
    24121 gggctggcgt tgctggtagg attttcaatt cctgacagag tcatggaagg aaaacatccg
    24181 caattttttg tcgagatgac acaagaatca cttcagccaa gccgctatat tcttactgat
    24241 agcgtcggtg ttgccgcagg tctggcatgg agcctgcaac gcgatgacat catcatgtat
    24301 cgccagacag gtgagttgaa atacggcctt aattatccgg atgcgaaagg gagatttgtc
    24361 agcggtgatg agttcgcaaa ctggcttaat caacatcgtc aggaggggat tattacactc
    24421 gtgctttcgg ttgaccgcga tgaagatatc aacagtctcg ccattccgcc cgcagatgcc
    24481 atcgttcgtc aggagcgtct ggtgctgatt cagtatcgtc ccaaatgatc tggctaacat
    24541 tagtctttgc cagcttgctt agcgttgccg ggcagttgtg tcagaaacag gcaacctgct
    24601 ttgtggcgat aaacaaacgg cgcaaacata tcgtgctgtg gctgggactg gcgctggctt
    24661 gtcttggtct tgccatgatg ctctggctgc tggtcttgca gaacgtaccg gtaggcattg
    24721 cctacccgat gttaagtctg aattttgtct gggtgacgct ggctgcagta aaactgtggc
    24781 acgaaccggt atcgccgcgt cactggtgtg gggtggcgtt cattattggt ggcattgtga
    24841 tcctcgggag tacggtgtaa tgtgcctgat atggggatta ttcagcgtca ttattgcctc
    24901 agttgcgcaa ttaagcctgg gttttgcggc aagtcatctg ccgccgatga cgcacttatg
    24961 ggattttatc gcggctctgt tagcttttgg cctggatgcc cggatactgt tactcgggct
    25021 gctgggttat ctgctttccg ttttttgttg gtataaaacg ctacataaac ttgcgctaag
    25081 caaagcctat gccttgctca gtatgagtta tgtgctggta tggattgctt caatggtact
    25141 tcccggctgg gaagggactt tttcgctgaa agcactactg ggagtagcct gtattatgag
    25201 cgggttgatg ctgattttcc tgcccacgac aaaacaacgt tactgagttt tccctgccgc
    25261 tgtacactgc cgttcccact catcggggct ataactgctt gccgagagga ctttcaccgt
    25321 atgcagcttt tcacgattaa tacaataaag cgcattctgc aaaggcgaga gtaaatcccc
    25381 cactttcacg gcgaagtcac tcttcacttc ggcgatcatt tttatcgcgc cgccagcatc
    25441 gcagagcata agtacatgtc tgttatcttg tttattgcag cacgattttt tgtgccagcc
    25501 attccattgc attatcctgt ttgctaagag ttttcacatc aatttacaga ttaacagata
    25561 aactctttat caacggcaat ggaatggcag aaatgagaag tgaaacggag ggttaattat
    25621 tgctgacgtt gcacccactc ttttagcgcc tgacgtgaaa ttttaatacc gccgtttttc
    25681 agctccggcg gtaaagtcag ccagcgcacc ggttgttgaa aacgagccag cttatctttc
    25741 acccattcgc caagatcaac ggtttgctgg tcatactcca ccaccgctac cggccgatga
    25801 ccaaactcct tgtcggcaac ggaaacgata aacacctgca aaaccgcagg atgtgcagca
    25861 ataacacgct cgacttcttc cggctgaata ccctcaccgc cactgaagaa cagattgtct
    25921 aaacgtccga caatggtcag cttgccatta tgcatctcac cgcgatcgcg cgtagcgtac
    25981 cagccttcgt cattaaccag tgaaaccagt tgcccgttac gccagtaacc ttctgccata
    26041 ctggcagccc gcagccacac ttcattatta acgattttca cttcccgacc cggcagcggc
    26101 aaaccaacgt ctgccaggcc gtcggcttct ttcgcacata ccgtggaggc aaactcggtc
    26161 agaccatagc cgcaaaagca acgaatcccc tgctcgcgcg cctgttccgt caactcgacc
    26221 gggatagccg cgccgccaag taacaccgct ttcagggaaa cggaactgcg gttaaccagc
    26281 aaacgccaga gttgtgttgg caccagtgaa gcgtgagtac agcctgccag catttgctcc
    26341 aatggctgtt tatcacgcac cgtcatccgc gcaccagcgt ataaccagcg ccacataatt
    26401 ccctgaccgg agacgtgaaa cagcggtaaa gagagcaacc agtcatcgtg atcgccaaac
    26461 ggaatcagcg ataacacacc ttgcgcactg gcaagatggg cctgataagt atggacagcg
    26521 gctttcggca aaccggtaga acccgaggtc aacgtcattg agcacagacg cgtcggctgc
    26581 cacgcagcgg catgtacgcc ttcaaccagc tgaatgtgca gcgacgttaa tgccggaaac
    26641 gtgttttccc catccggcac cagcgcaaat tgcagcgtca gattgggcag caattcttca
    26701 agcaacggtt gcggcagctg agggttcacg ggcaacaccc gcgccccgca ttgcagtaac
    26761 gccagccagg cgagcagcgt ttgcggcgta ttccacgccc gcaacatcac gccgctgccc
    26821 tcaaccaccc cctgcgccgc aaagcccgta gctaactcat cgacgcgagc acaaagctcg
    26881 cgccagttga gttgctcgtc attaagacgt aaggcgatgg cttctccccg cacttgccgc
    26941 cagtgacgcc acggccagtc agagaagatc ataacaactg ctcctgtaca tcagcgtcca
    27001 ctaacggcag tgggctcccc ggccagcgac gaacctgctg cgcctgcatc agatccagcg
    27061 tgtccagtcc cggaatggtg tctggcgtta accaggcagc aatccgcgcc agttgcgtta
    27121 gacctaagct cgactcaatg gaagaactga tcaccgccgt cagccccagc gcatgcgccg
    27181 cctgtacctg ctcgcgtact ttttccagac tgcccgtgag cgtgggtttg ataactaccg
    27241 cgcgcacgcc ctcttcagcc acaaaggcaa aatccggctc gcgcaggctt tcatcccagg
    27301 caatggcaat gccggtttca cgggcaaacg ctcgcgaatc atcgcgggtt ttgcacggct
    27361 cttcgagaaa cgcgatgcgg tggcgataat ccgggttaac atatttggca aactgctgac
    27421 ctttcagcgg tgtccaggcg cgatttgcgt caagacgcaa atgcagatcc ggaattgcct
    27481 ccagcaacag attcaccacc atgccgtcgc gcaccgcttc gtacaatccc actttgacct
    27541 tcgccacttt ctcgcccggc atatcggcaa gtttgaggat cagatcgtcc ggatcgccat
    27601 tacacagcgg tgccgcacgg tagttggctg cttgcggcaa cgtatctgtc agttctgcca
    27661 atgcacagct tacgccaaag gccacggaag gcatctgcgg tagctcgcaa tcgcctgcca
    27721 gccagttatt tacccaggca agcagcacgc tttgcgcctc ttcccagttt tcctggctga
    27781 agcccggcag tggggagatc tccccccacc cttcgcgctc gccctcacgc aggcaaacgt
    27841 acagcccgtc gcgggttttt aaccgcctgt cgcgcagaac cacccccgcg tccatgggga
    27901 tctgccagcg gtatacctgc gcgctacgca ttacggattc cgtttaaatt tgctgaaatc
    27961 aggctgacgt ttctggttga aagcgttgcg accttcctga ccttcttccg tcatgtagaa
    28021 cagcatggtg gcgttgcccg ccagctcctg cagccccgcc tgcccgtcac agtcggcgtt
    28081 cagcgcagct ttcaggcagc gcagcgccat cgggctgttt tgcagcattt cgcggcacca
    28141 acggacggtt tctttttcca gatccgccag cggtaccacg gtgttgacaa ggcccatatc
    28201 cagcgcctgt tttgcgtcgt actgacggca caggaaccag atttctcgcg cttttttctg
    28261 cccgacgata cgagccatgt aggaagcgcc ccagccgccg tcgaaggaac cgactttcgg
    28321 gccagtctga ccgaagatgg cattatctgc tgcgatagtc aggtcgcaca tcatgtgcag
    28381 aacgtgacca ccgccgatgg agtagccagc caccatcgcg acaaccggtt tcggacaggt
    28441 acggatctga cgctggaagt ccagcacgtt caggtgatgt acgccggaat catctttata
    28501 gccgccgtaa tcaccacgca ctttctggtc accaccggag cagaacgctt tatcgcctgc
    28561 gccagtcaga atgatcacgc cgatattgtc gtcataacgc gcatctgcca gtgcctggat
    28621 catctctttt accgtcagag gacggaaggc attgcgcacc tgcggacgat taatggtgat
    28681 ttttgcgata ccgtcggtgg atttttcata acgaatgtcc tcgaaacctt cggagcagtc
    28741 gtgccattca accggtgcgt aaggcattgc ttcatcagga taaatcatat tgtgtccttt
    28801 agtcagaaac gcaagatctg cgccagactt gcgattacgc cagcgggatt ttcccgatgc
    28861 gcgttatgtc cggcgcgagg aatgacatgg cagtcggcag ccagttccgc cgccagggcg
    28921 cggaatttgc tgtcacgttc accacataaa taataaaacg caaatgtgcg ggcgctaagg
    28981 ttagcacgta aatcaggctg gacggcgaga gaagtcgcct ccagcatggc ggcaagggtt
    29041 gcgccattat tgttgctgcg cagcgccacc agctcccggc gttgatcgtc attgagcgag
    29101 gcaaaaacag gctgttgata ccagtcggca aataccgccg ttaacggttc tgtgcgaaaa
    29161 cgctgcgccc attggcgatc ggaacgctga cgttccgcac gttgttcagc attttgcagc
    29221 cccggatgcc cgccttcgac aataacccca caaagccccg ccagcccctg gcaagccgcc
    29281 atcatcgcca cccgtccgcc aagcgagtac cccaccagcc agaagtcaag gatgttgtaa
    29341 ctaaccaagg ttttacgcag taagtcggtg acatcatcaa atccatcgac gctaatcgcc
    29401 gccgaaccac catgacctag gagatcaaca tacaaccgtg agtagtcggc aaacgcctcg
    29461 cccacttctt gccattcgtg gcaatcgccg gaaaaaccgt gcaaaaacac cagccagggt
    29521 aaacctagtt ttccgtgttt tgcctgcgcg tgcaggatca taaatggctt acctgcgcca
    29581 gaagttgctg gagcgtttgc gcaccatcgg tgtcgttaac caccatttca atcaccgtgg
    29641 tggttggcgt gcgccaggcg tcggcaaatg ccgtttcaag ttcctgccag ttttgcggat
    29701 gatgatattt cagctcgaac atcgcggcgg cgtgctcaaa atggacgttt tgcggcatca
    29761 gatagaaacg ctcacgctcg ctttgcggcg ttggcaacag cgagaaaatt tgcccgccgt
    29821 tgttgttcac cacaattaat accagcggcg cagaaacctg acgcaataac gccagcgcgt
    29881 tgagatcgta aagcgcggag agatcgccca caatcgccag cgtcggtttg ccgcttgccc
    29941 gctgaacgcc tgcggcggtc gaaagcagtc cgtcgatacc gctggcccct cggttgctgt
    30001 acaccgggta acctgccgga agttgcgaaa gcgcatcaat cagacgtacc accaggctgt
    30061 taccaacaaa caattgcccc tgttcaggca gatagtcgct gatgcgatgc gccagttgcg
    30121 cttcgccaaa cgcatcacgg cgggcaataa ccgcctgcat tgcctgttcc gccaggcgcg
    30181 ggatttcaac gcaccagggc tggcgttttt ctgccggatg cagctccagc cagtcggcaa
    30241 tattggcaat taagcgacgt ccgcgatggt gtgccggatc aagtcgccct tcaatgtcat
    30301 caacaatcca gtactcttct ggttcacagc ttgcctgcca ttgcaggagc cgtttccccg
    30361 tcaggctact tcccagttgc accacaattt gcgcctgctg cagctcgctg gtcgctttgg
    30421 cattgcctaa ccaaagatcg gcacacggca gcggctgccc ggtttgtgac agcacatcgc
    30481 caatcagcgg ccagccaaga gtttgcgccc acagggcaac ttttttgccc tcttccgcac
    30541 tcatgcgccc ggcaaccacc acgccgcgct tttgtcgcca gaagaaccag tcgcgctgtt
    30601 tttcactttc cagacgaggc gcttcacgca gccacggttt gtcgtcctgc caccagtcac
    30661 ccagacgctg ttgccagcta agcccggtat cgtccatttc gccatacagc ggttcagcaa
    30721 acgggcagtt gatatggact ccccccgcat gaagcgtacc gagagcgtgg tcgatggtag
    30781 aaaccagcca acgtgcgggg atatcctggg tcgggcgcgg caatgaaata ctgtgcgtgg
    30841 ggtgagaggc gaacattccc ggctggcgaa ttgcctgatt cgcgccgcag tcaattagct
    30901 ccggcgggcg atcggcggtt aagagaatca gtttttctcc ggttaacccg gcttcaatca
    30961 gtgccggata gagatttgcc accgccgtgc cggaggtcac aatcaccgcc accggctgct
    31021 tgctgacttt cgccagcccc agcgccagat gccccaaccc acgctcatcg aaatgggtgt
    31081 ggtgaatgaa tgcggaattc tccgccgccg ctaacgttaa cggtgtagaa cgcgagcctg
    31141 gggcgataca gatgtgtctg acgccgtgac gcgttaatgc ttccagaatg accgccgccc
    31201 agcgtcggtt aaatgcgctt actgacatga gtttgtccgg tatcaatatt gcggctaagt
    31261 ataaggagct acaaaaatag attattgata tgaatcggta atgatgcgac tcattactat
    31321 tccatttgta ataaagtacg cagccctgcc gctttgttgt cgatttcctg ccactcttgc
    31381 tcggggtcgg aaccacggac aatgcccgcg ccagcatata atcgcacgac attgccgcta
    31441 atttttgctg agcgcaggga aacgcagaat tcgctttgtt gtaatgagag atagcccgca
    31501 gaaccggcgt accattctcg ggtgaacggt tcgtgacggg cgataaactg tcgcgccaga
    31561 tcgcgcggta agccagcaac tgctgccgtt ggctgcaact gatgtaaaca gatcacatca
    31621 tccgctttgt tgagtgaagt ccagatacag cggcgaagat gctgcacttt acgcagacgc
    31681 agtacctgcg gcggtaaaac atccagcgtc tgggtatcgg cctgtaatcg ttggcagata
    31741 tcttccacca ccagcatgtt ctcgcgctgg tttttatcat ccgccatcag ccactctcct
    31801 aactgctgcg cctgcttatc atcaggatga tttgctactg ttcccgccag cgcttcagta
    31861 cgcagcgctt tgtcacgccg ccgccataac cgttccggtg aagagccaag aaaagcattt
    31921 tcgccatcaa aggccatgta aaaatggtag caattcagat tcagtcgacg actggcagcc
    31981 atcatcgccg ccgcgttgac cggacttgcg aaatgcaggt cagttgcccg agcgagcacc
    32041 actttgtcga gctcaccttc ggcgatggtt ttcgttgcca gttcgattaa ttgcgtccag
    32101 cccgttttgt ccggccagtg ttgttctcgc gtggtggtta aatgtaaccc aggcaagggc
    32161 ttgatactca ccagtgtggc gataaattct tttgcctgaa tcgcatcgtg ctgaagggag
    32221 ctttcgctga atagcgtcag ccgcagcgtg gctttaccgc cacagcgtcg ccattccagg
    32281 cggggtaaaa gtaaattgcc ctgcgacggg tcaaaagcat tcagccccca aatgcgtaag
    32341 tcagcgtgtt ccgggtgctg gcgaagaaaa cgttgtgcct ggtccaacga cgtaaaacgg
    32401 gtaatcgcgc ccaggacggc agcttcttca tcaccattac gttgttgcca gtagaattgc
    32461 gggtacgttt gctgactggc cagccagctc aaggcatcaa aagcgtcttt gagagggaaa
    32521 ggaatatcga taacccgaat gccgggtgtc gccggaatct cttgcgacaa atggcgcagt
    32581 agattttcca gcgccgtagt aagtgattgc acgcggacct ctccctgtta aaagcctcac
    32641 attatacggg gtactacaaa aaaaatgcag taccccggtg tagggaggtt ttaacggcgt
    32701 gccagcaaca gtcctagtac cagcccgacg gccgcgccca caccaattcc ttgccagggt
    32761 ttttcgtgga cgtagtcatc agcacgataa acagcctgct tcgcccgata gtaataactg
    32821 tctgaagcct ggctaacccg ttttttcaca tcatccagcg ctttttctgc atgcgctttc
    32881 agctcaacat atttctgatc ggcgggatcg ccagaggagc ggagcacctc ttccagtgtt
    32941 tcactaagca gcgtcaggtc gtcatcgata cgtgtatcac caaactgatt agacattctc
    33001 gttctccatt tgcgtaaaac ctgtcactaa ctatagacaa gagttgttga ttacgcctga
    33061 attacctcgc gtgccatccc aatgtgtggg ataccatctt cttcatagac ctccgtcaca
    33121 gggataaaac cgaaactctg gtagaagttt tgcagatgcg cctgcgcccc gaggtaaaca
    33181 ggcttatcag gccagtgatg cgtacatgtt tcgagtgttt tactcatcag ctgctgcccc
    33241 actttttcac cacgcaacgc ttcactgaca atcacccgac ctataacgac cggctcaaga
    33301 tcatcatcac ttttcagaat cctcgcatac gccaccagtt catcattttt ccaaccgaga
    33361 atatggcgat tatcccccgt caaatcatca ccatcgatat cctgataagg gcaattctgc
    33421 tcgacaacaa acaccgcgca acgcagttgt aataaggcat aaagctggga aaccgataat
    33481 tcagagtgat gcagatcttg ccattcaatc ataatgctct ccttttagat gtatgcctta
    33541 tactagccgg aatatttttt gaaacgtgat gaactcatgg aattaatttt tttaggtact
    33601 tcagccggtg tgccaacccg cacgcgcaat gtcacggcaa tattgcttaa cctgcaacat
    33661 ccgacccaga gcggactttg gttgtttgac tgcggtgaag gcacccagca tcagctactg
    33721 cataccgcct ttaaccctgg aaaactggac aagattttta tcagtcacct tcatggcgat
    33781 catctttttg gtttacccgg cttgctgtgc agtcgttcta tgtcaggcat tatccaaccc
    33841 ttaacgattt atggtccgca aggtatccgt gaatttgtgg aaaccgcgct gcggattagc
    33901 ggctcatgga ccgattatcc gctggaaatt gtcgaaattg gcgctggcga aattctcgat
    33961 gatggcctgc gcaaagtaac cgcttatccg ctggaacacc cactggaatg ttatggctat
    34021 cgtattgaag aacatgataa accgggtgca ttaaatgccc aggcattaaa agctgctggc
    34081 gtgccgcctg gcccactgtt tcaggaatta aaagcgggca aaacaatcac gctggaagat
    34141 ggaaggcaga ttaacggcgc agattactta gctgctccag tgccaggtaa agcgctcgct
    34201 attttcggcg ataccggccc ctgcgatgcc gcacttgacc tggctaaagg tgtcgatgtc
    34261 atggtgcacg aagcgacgct ggatataacc atggaagcca aagccaatag tcgcggccat
    34321 agctctacac gccaggctgc gacactagcc cgtgaggctg gagtcggcaa gctaatcatt
    34381 acccacgtca gctcgcgcta tgatgacaaa ggttgtcaac acctgttacg tgaatgcagg
    34441 tcaattttcc cggcgactga actggcgaat gatttcaccg tgtttaacgt ttaactctgc
    34501 ttattattaa aaacagggtg aaacttgccc tgttatagca acccgcgcta catatatcca
    34561 cccacacagg catattgagc gatttttgat atttattcat ctccttacgc actatattca
    34621 gagcaataag ttgcctcttt tcatcttgat tcagcacaac ctttgcaact aaaaggagcc
    34681 catgatggtt acagttgtca gcaattattg tcaattatct caaacccaac tcagtcagac
    34741 atttgcagaa aaatttactg tgaccgagga attactgcag tctttaaaaa aaacagcgtt
    34801 atccggagat gaagaaagta ttgagttact gcataatatt gcgttaggtt atgatgaatt
    34861 tggaaaaaaa gctgggtaat gctgccaact tactgattta gtg
//
LOCUS       DAEBXN010000034        34699 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.034, whole genome shotgun sequence.
ACCESSION   DAEBXN010000034 DAEBXN010000000
VERSION     DAEBXN010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34699
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.034"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..2099
                     /locus_tag="KJL99_001712"
     CDS             <1..2099
                     /locus_tag="KJL99_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HBD4436468.1"
                     /translation="FNGYDADDVSDSNTDVSDLYYRYDFLRRYYVQAGRMDNRTLFNA
                     QGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGTPVMVLLSRNSRVDAYRNEQLLG
                     SFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRTELVPFTKTGGLTDGNAQWFLQA
                     GKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANADDVSAFELGNNWTADLGRVGNL
                     AISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTNSDGDACTTDSRESYNALSCYES
                     ISATVSLNFVGWNMMLGYTCTQNNTDDSLRWDKQQSFENNYLRQTTAQSISETVQLSA
                     SRAIVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSFSLSDTPTMDSNNNSHSTNVPTD
                     YRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGLNTDTINSAVNGRWDGQYGNVYA
                     TVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGASGTDDLLGAVLVDVKGFSEQDE
                     ESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGFVEVNDSSQGNQQGTTNIINGAG
                     NRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKKFPIVGLNSAMLLVAEDGGFTLE
                     INGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDVTCSVVTYSQLPESIQVQAQLKQ
                     PKLRGNVQTAQREVAP"
     gene            2096..3184
                     /locus_tag="KJL99_001713"
     CDS             2096..3184
                     /locus_tag="KJL99_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HBD4436469.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPLGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(3272..4570)
                     /gene="exuT"
                     /locus_tag="KJL99_001714"
     CDS             complement(3272..4570)
                     /gene="exuT"
                     /locus_tag="KJL99_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HBD4436470.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            5055..6467
                     /gene="uxaC"
                     /locus_tag="KJL99_001715"
     CDS             5055..6467
                     /gene="uxaC"
                     /locus_tag="KJL99_001715"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HBD4436471.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            6482..7969
                     /gene="uxaA"
                     /locus_tag="KJL99_001716"
     CDS             6482..7969
                     /gene="uxaA"
                     /locus_tag="KJL99_001716"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HBD4436472.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQLDFQDLPAQ
                     AADPEVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            8052..8603
                     /locus_tag="KJL99_001717"
     CDS             8052..8603
                     /locus_tag="KJL99_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HBD4436473.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(8608..9852)
                     /gene="sstT"
                     /locus_tag="KJL99_001718"
     CDS             complement(8608..9852)
                     /gene="sstT"
                     /locus_tag="KJL99_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HBD4436474.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPFGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(10448..11413)
                     /gene="alx"
                     /locus_tag="KJL99_001719"
     CDS             complement(10448..11413)
                     /gene="alx"
                     /locus_tag="KJL99_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HBD4436475.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(11684..12682)
                     /locus_tag="KJL99_001720"
     CDS             complement(11684..12682)
                     /locus_tag="KJL99_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBD4436476.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKAGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLGSEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKLYLPK"
     gene            complement(12761..13453)
                     /locus_tag="KJL99_001721"
     CDS             complement(12761..13453)
                     /locus_tag="KJL99_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HBD4436477.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWGDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRT
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            complement(13530..14033)
                     /locus_tag="KJL99_001722"
     CDS             complement(13530..14033)
                     /locus_tag="KJL99_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HBD4436478.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGSKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            14118..15254
                     /gene="rlmG"
                     /locus_tag="KJL99_001723"
     CDS             14118..15254
                     /gene="rlmG"
                     /locus_tag="KJL99_001723"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HBD4436479.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     LGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHYARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            15539..15853
                     /gene="higB"
                     /locus_tag="KJL99_001724"
     CDS             15539..15853
                     /gene="higB"
                     /locus_tag="KJL99_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HBD4436480.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            15850..16266
                     /gene="higA"
                     /locus_tag="KJL99_001725"
     CDS             15850..16266
                     /gene="higA"
                     /locus_tag="KJL99_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HBD4436481.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(16311..18329)
                     /gene="fadH"
                     /locus_tag="KJL99_001726"
     CDS             complement(16311..18329)
                     /gene="fadH"
                     /locus_tag="KJL99_001726"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HBD4436482.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQGCLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(18555..20906)
                     /gene="ygjK"
                     /locus_tag="KJL99_001727"
     CDS             complement(18555..20906)
                     /gene="ygjK"
                     /locus_tag="KJL99_001727"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBD4436483.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVNFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVNYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(20923..21342)
                     /locus_tag="KJL99_001728"
     CDS             complement(20923..21342)
                     /locus_tag="KJL99_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4436484.1"
                     /translation="MNGYIQYDLAEGITWMNGLEITDGTGQLYLTGLFTPNFAARAWH
                     HTGRADGLDVPGSESGMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIW
                     YEYGGGRFATAFDSRFYMKNASNDPSDQIFLMQYFYW"
     gene            complement(21320..21673)
                     /locus_tag="KJL99_001729"
     CDS             complement(21320..21673)
                     /locus_tag="KJL99_001729"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4436485.1"
                     /translation="MPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWW
                     GDFTPDLGYVIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYD
                     DKYTYPAMDERLHPV"
     gene            complement(21674..21994)
                     /locus_tag="KJL99_001730"
     CDS             complement(21674..21994)
                     /locus_tag="KJL99_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4436486.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGLGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNA"
     gene            complement(22128..23561)
                     /locus_tag="KJL99_001731"
     CDS             complement(22128..23561)
                     /locus_tag="KJL99_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBD4436487.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(23624..24073)
                     /locus_tag="KJL99_001732"
     CDS             complement(23624..24073)
                     /locus_tag="KJL99_001732"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HBD4436488.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(24070..27156)
                     /gene="ebgA"
                     /locus_tag="KJL99_001733"
     CDS             complement(24070..27156)
                     /gene="ebgA"
                     /locus_tag="KJL99_001733"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HBD4436489.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SSQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGSGLKEYKQVIAPVKIHALTRGELKVENKLWFTTLD
                     DYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSRTR
                     YSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKMSG
                     KPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQSD
                     GEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFTMG
                     INGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRWTA
                     LTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLGSN
                     SWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ"
     gene            complement(27339..28322)
                     /gene="ebgR"
                     /locus_tag="KJL99_001734"
     CDS             complement(27339..28322)
                     /gene="ebgR"
                     /locus_tag="KJL99_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HBD4436490.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            28540..28871
                     /locus_tag="KJL99_001735"
                     /pseudo
     CDS             28540..28871
                     /locus_tag="KJL99_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
     gene            complement(28913..30292)
                     /gene="ygjG"
                     /locus_tag="KJL99_001736"
     CDS             complement(28913..30292)
                     /gene="ygjG"
                     /locus_tag="KJL99_001736"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HBD4436491.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGVFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            30710..32230
                     /gene="aer"
                     /locus_tag="KJL99_001737"
                     /pseudo
     CDS             30710..32230
                     /gene="aer"
                     /locus_tag="KJL99_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
     gene            complement(32281..32904)
                     /gene="nfeR"
                     /locus_tag="KJL99_001738"
                     /gene_synonym="yqjI"
     CDS             complement(32281..32904)
                     /gene="nfeR"
                     /locus_tag="KJL99_001738"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HBD4436492.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            33181..33926
                     /gene="nfeF"
                     /locus_tag="KJL99_001739"
                     /pseudo
     CDS             33181..33926
                     /gene="nfeF"
                     /locus_tag="KJL99_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
     gene            complement(33980..34055)
                     /locus_tag="KJL99_001740"
     tRNA            complement(33980..34055)
                     /locus_tag="KJL99_001740"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34020..34022),aa:Ile,seq:cat)
     gene            34180..34686
                     /gene="mug"
                     /locus_tag="KJL99_001741"
                     /pseudo
     CDS             34180..34686
                     /gene="mug"
                     /locus_tag="KJL99_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
BASE COUNT         8472 a         8924 c         9105 g         8198 t
ORIGIN      
        1 atttcaacgg ttatgatgca gatgatgtca gtgacagtaa tactgacgtc agcgatctct
       61 attatcgtta tgatttttta cgtcgttatt atgtgcaggc gggtcgcatg gacaaccgca
      121 cactatttaa tgcacaaggc gggaacttta cctttaactt tctgccactc ggtgcaatcg
      181 atgggatgcg tatcgggtcg acactgagct atttaaacca ggcgcaaagc cagcaaggaa
      241 ccccagtaat ggttctgctt tcgcgcaatt ctcgtgttga cgcttatcgt aatgagcaac
      301 ttttgggatc gttttatctc aatagtggtt cgcaatttat tgataccagt tcctttccgc
      361 caggcagcta cagcgtggca ttaaaagtct acgaaaataa ccaactcacc cgcaccgagc
      421 ttgtgccgtt taccaaaacc ggcggtctga ctgacggaaa tgcgcaatgg ttcttacagg
      481 caggtaaaac tacatcacag gtttctgatg atgaaagttc agcttatcag ctaggagtac
      541 gcctgccatt acatccgcaa tatgagctct acgcagggct ggcgaatgct gatgatgtga
      601 gtgctttcga gttaggtaat aactggacgg cagatttagg cagggtgggg aatcttgcaa
      661 tcagcgccag cgtgttccgt aacgatgacg gcggcaaagg tgatatgcaa caggccaact
      721 ggagtaatcc gggatggccg acgttgggct tttatcggac caactctgac ggcgatgctt
      781 gtacaaccga cagcagagag agctataacg ccttaagctg ttatgaaagt atttccgcga
      841 cagtttcact gaattttgtc ggctggaata tgatgctggg ttatacctgc acacaaaata
      901 acactgatga tagtttgcgt tgggataaac agcagagctt tgaaaataac tatcttcgcc
      961 agacaacagc gcaaagtatc tctgaaactg tacaacttag cgcttcccgc gctattgtga
     1021 tgcgtgactg gattttgagt acgtcagtcg gtgttttcca tcgtaatgac aacggtggcg
     1081 ataacgacga caacggcttg tacttatcgt tttcgttatc tgacacgcca acgatggata
     1141 gcaataacaa cagccattca accaatgtgc ctacggatta tcgttatagc gaacaggatg
     1201 gcgatcaaac gtcatggcag ttatcgcata ccttttataa cgattcattc agccataaag
     1261 aacttggcgt aaccgtcgga ggcctgaaca ccgataccat aaacagcgcg gttaacgggc
     1321 gttgggatgg gcaatacgga aatgtctacg ctaccgtatc tgatagttat gaccgtaaga
     1381 atcatgatca tctttcggcc tttacgggca cttatagctc tacgctggct gtcagtcgct
     1441 atggcgttaa tttgggagcc agtggtacag acgatttgct gggtgcggta ctggtggatg
     1501 tgaaaggctt ctctgaacag gatgaagaga gtcaggatct gcaactcgaa gcgcgggtgg
     1561 caggcagccg aactttgcag cttggtcaaa gtgacagtgt gttgttccct tatcctggat
     1621 ttcagtctgg ttttgttgag gttaacgaca gtagccaggg caatcagcag gggacaacaa
     1681 acatcattaa cggtgcaggg aatcgtgaat taatgttgtt gcctggcaag ctgcgctatc
     1741 gcgaagtgtc tgccagcttt aactacaact atatcggtcg tttattactg ccggcagcgg
     1801 tgaaaaaatt cccgattgtg gggctgaaca gcgccatgtt actggtagct gaagatggcg
     1861 gatttacact tgaaattaac ggcagcgaaa aagagctcta tctgctttcc gggcagcaat
     1921 tccttaagtg tccactgagt gttgtaaaga aacgcgccag cattcgttac agcggagatg
     1981 ttacttgtag tgtggtgact tattcacaat taccggagtc gattcaggtt caggcacagt
     2041 tgaaacagcc taaattacgt ggaaacgttc agacggcgca aagggaggtt gcaccatgag
     2101 aaatcgattg attgcggcga tattgggctt gtttggcacg ctcactggcg ttcaggcagc
     2161 tcctgacgtg accagtgaaa ttacgtatga tttggcatct ggtagagcgg attattactt
     2221 ctggaaggat gaagcgtcgg caggaaataa tggatatatg tggtatgaat gttcgtatcc
     2281 tgacctccaa caaacctgta cagctaatgg aaatatatcg acagtacaaa tctatttaac
     2341 tgaacaacgc agtgggatgc gttggccggt aaaactcaaa ggatttaaaa cagccattgt
     2401 aagtagcgat gaagcgccgc taggatgcaa ggggggcaaa gggcttcaga cgaatcttaa
     2461 ggattctaat agatcttcat gtacagaaga tggtcaacat tattatatat acgatacaaa
     2521 gtttcttacg ctctaccttg agcagacaga gatgaagaat ttgccgattg gtggcgtctg
     2581 gaaggggaaa gttaaattac attcgaacag cccggcccag gactatttcg caaatattac
     2641 cctgaatacg ctcgacccca accatattga cgtgttcttc ccggagttcg cccacgccac
     2701 gccaagggtg cagttagact tgcatccaac aggaagcgtt aacggcagca actacgcgca
     2761 agatctgacc atgttggaca tgtgcctgta cgatggtttt aacggtaatg ccatcagtta
     2821 tgaaatcatg ctcaaagatg aagggcgacc agctgcaggg cgcagagacg gttacttctc
     2881 tatctatcgt cagggaggga ccaccaccga cgagggagaa cgcattgatt accgggtcaa
     2941 aatgtacaac ccggaaaccg gtgggcaaat tgatgtgcgc aataatgaaa atatggtctg
     3001 gaacagcatt aacctgaaac gtgtgcgtcc ggtggtactg cccggtattc gctatgccgt
     3061 gatgtgtgtg ccgacaccct tgactctggc agtcgataaa ttcagcgtga tggataaaca
     3121 ggccgggtac tacatgggca aattgtcagt aatctttacg ccttccttgc caaccatcaa
     3181 ttaaccctgc aaaccggaca aacgcttttt tctatataaa cgacgaagcc gcctgataag
     3241 gcggcttcgt cgttataact tacgttaaac gttaatgttg cggtgcggga tcgttatgtg
     3301 tttcctgcgc cacctcaatc gccggtttgt tctgcaatac ggtccagata accagcgcac
     3361 ccagcaggtc aaatactgcc agaactgcga acagcgggct gaagccgatg gtgtcagcca
     3421 gtgcaccgac aaccagcgca aacagcgtac ttgccagcca tgcggacatc ccggttaaac
     3481 cgttcgccgt cgccacttcg ttacgaccga acacgtcaga agagagcgta atcagcgcgc
     3541 cagacagtgc ctggtgggca aaaccaccga tacacagcag cataattgcg acatacgggt
     3601 tggtgaacag accgatcata cccgggccaa tcatcagcac agcacccagc gttacgacca
     3661 tcttacggga aacgatcagg ttcacaccaa accaacgctg gaacagcggc ggcaggtaac
     3721 caccgaggat acaaccgagg tcagcaaaca gcatcggcat ccaggcgaac atcgcgatct
     3781 ctttcaggtt aaagccgtaa actttaaaca tgaacagcgg gatccacgcg ttaaaagtac
     3841 cccaggccgg ttctgcaagg aaacgcggca gtgcgatacc ccagaactga cggttacgca
     3901 ggatctgacc aacggacatt ttcttcgccg tgctcacctg gtgctgggct tcctgaccat
     3961 taataatata gtcgcgttct tcatcggtca gatgcttctg gtcgcgcgga tgtttataga
     4021 aaatcagcca tgccatcgcc cagataaagc tcaatgcacc ggagatgata aatgccatct
     4081 gccagctgtg catcacgatt gcccatacca ccagcggcgg cgcaatcatc gcaccaatcg
     4141 aagaacctac gttaaagtag cctactgcga tggaacgctc tttcgccggg aaccattcgg
     4201 agctggcttt cagacccgcc gggatcatcg ctgcttccgc ggcaccgacc gcaccacgag
     4261 caacagctag gccaccccag ctacctgcca gcgcggttgc accacagaac acggcccaca
     4321 gcacagcaaa cattgcataa ccgattttcg tacccagcac atccagtaca taacctgcta
     4381 ccggttgcat gaccgtataa gcagcagaat aggctgcgat gatataggaa tactgttggg
     4441 tggagatgtt taactcttcc atcagagttg gcgcagctgc cgccacagtg ttacgcgtca
     4501 ggtaaccaag cacggtgccg agcgtcacca gtgcgatcat ataccaacgt aaccctttaa
     4561 ttttacgcat gtaaaacctc atcgttttgt tatatccgca ggaagcgcgg ccatcatccc
     4621 gccagaggct gaagatgtta gttacgaccg ggtaaccgaa aaaaaaaacc ggcacgcccc
     4681 gaacgttgcc atgaaagttt cgtgctaatt cggcttccgt accggttaac ccgattcatc
     4741 taacaaatga atgaatcggc aatttgcatt ccgtcgactt atgatttgcg acggcagaaa
     4801 gataacttgt catacaacct taaaaggtga gagccatcac aaatgtgcga atatttgtag
     4861 ggacattacc tgacgacagc gaggccagta ctggcgcggc ctgcagcgag atttaccact
     4921 ttgagagtaa tttttttaac tacgtttatt gatctaactc acgaaaatat cttcggactc
     4981 tggaaattgg tgtgataact ttgtcagcat cgcaccataa gcaagctagc tcactcgttg
     5041 agaggaagac gaaaatgact ccgtttatga ctgaagattt cctgttagat accgaatttg
     5101 cccgccgtct gtatcacgac tacgcaaaag accagccgat tttcgattac cattgccatt
     5161 tgccgccgca gcagattgcg gaagactatc gttttaaaaa cctgtatgac atctggttga
     5221 agggcgatca ctacaaatgg cgcgctatgc gtaccaacgg tgtggctgag cgtctgtgta
     5281 ctggtgatgc gtctgaccgt gaaaaatttg acgcctgggc ggcgactgtt ccgcacacta
     5341 tcggcaaccc gttataccac tggacgcacc tcgaactgcg tcgtccgttc ggtatcactg
     5401 gcaaattgct ttctccgtca actgccgatg aaatctggaa cgaatgtaac gaactgctgg
     5461 cgcaggataa cttctccgcg cgcggcatca tgcagcagat gaacgtgaaa atggtcggca
     5521 ccaccgatga cccgatcgat tctctggagc atcacgcaga gatcgccaaa gacggctctt
     5581 tcaccatcaa agtgctgccg agctggcgtc cggacaaagc cttcaatatc gaacaggcga
     5641 cctttaacga ctacatggcg aagctgggcg aagtttccga taccgacatt cgccgctttg
     5701 ctgacctgca aactgccttg accaaacgtc tggatcactt cgccgctcac ggctgtaaag
     5761 tgtctgacca tgcgctggac gtggtgatgt ttgctgaagc gaacgaagcg gaactggaca
     5821 gcattctggc gcgccgtctg gctggcgaaa ccctgagcga gcacgaagtg gcacagttca
     5881 aaactgcggt actggtgttc cttggtgccg aatatgcacg tcgcggctgg gtacagcagt
     5941 accacattgg cgcgctgcgt aataacaacc tgcgtcagtt caaactgctg gggccggatg
     6001 taggctttga ctccatcaac gaccgtccga tggcggaaga gttgtctaag ctgctgagca
     6061 agcagaacga agaaaacctg ctgccgaaaa ccattctcta ctgcctgaac ccgcgcgata
     6121 acgaagtgct gggcaccatg atcggtaact tccagggcga aggtatgccg ggcaaaatgc
     6181 agttcggttc cggctggtgg tttaacgatc agaaagacgg tatggaacgt cagatgaccc
     6241 aactggcgca gctcggtctg ctgagccgct ttgtcggtat gctgactgac agccgtagct
     6301 tcctgtcata cacccgtcac gaatacttcc gccgcattct gtgccagatg atcggtcgct
     6361 gggtggaagc gggcgaagca ccggcggaca tcaacctgct gggcgagatg gtgaaaaata
     6421 tttgctttaa caatgcgcgt gactacttcg ccattgaact gaactaaggt ctggggttga
     6481 tatgcaatac atcaagatcc atgcgctgga taacgtcgcg gtcgctttag cagatttggc
     6541 tgaaggcaca gaagtcagtg tcgataacca gactgttacg ctgcgccagg atgttgctcg
     6601 tggacataaa tttgcgttaa cggatatcgc aaaaggggcc aacgtcatca aatatggcct
     6661 gccgattggt tatgcattgg cggatattgc ggcgggggaa cacgtccacg cccacaatac
     6721 gcgcacgaat ctgagcgatc tggatcagta tcgctatcaa cttgattttc aggatctgcc
     6781 tgcgcaagcg gcagatcctg aagtgcagat ctatcgtcgc gctaacggcg atgtcggggt
     6841 gcgtaacgag ctgtggatcc tgccaaccgt gggctgtgtc aacggcatcg cgcggcaaat
     6901 ccagaaccgt ttcctgaaag agaccaacaa cgccgaaggt accgacggcg tgttcctctt
     6961 cagccacacc tatggctgct cacaactggg cgacgatcac atcaacaccc gcaccatgct
     7021 gcaaaacatg gtgcgccacc cgaacgcggg cgcagtgctg gtgattggtc tgggctgtga
     7081 aaacaaccag gttgccgcat tccgtgaaac gttgggcgat atcgatcctg aacgcgttca
     7141 tttcatgatc tgccaacagc aggatgatga gatcgaagcc gggatcgagc atttgcatca
     7201 gctgtataac gtgatgcgca acgataaacg cgagccaggc aaactcagcg aactgaagtt
     7261 tggtctggag tgcggtggtt ctgacggtct ttctggtatt actgctaacc cgatgctggg
     7321 gcgtttctct gactacgtga ttgctaacgg cggtactacc gtactgaccg aagtgccgga
     7381 gatgtttggc gcagagcagt tgctgatgga ccattgccgc gacgaagcaa cgtttgaaaa
     7441 actggtcacc atggtcaacg acttcaaaca gtactttatt gcccatgatc agccgatcta
     7501 cgaaaaccca tcaccgggga acaaagcggg cggtatcacc acgctggaag acaaatcact
     7561 tggctgtacc cagaaagcgg gttccagtgt cgtggttgac gtgctgcgtt acggcgagcg
     7621 tctgaaaacg ccagggctga acttgttaag tgcgccgggt aacgatgccg tagcgaccag
     7681 cgccctggcg ggtgcgggct gccatatggt gctgttcagt actggtcgcg gcacgccata
     7741 cggtggattt gtgccgacgg tgaaaatcgc caccaacagt gaactggcgg cgaagaaaaa
     7801 acactggatc gactttgacg cgggtcagct gatccacggt aaagcgatgc cgcagttgct
     7861 ggaagaattt atcgacacca tcgttgagtt tgccaacggt aagcaaacct gcaacgagcg
     7921 taacgacttc cgcgaactgg cgatcttcaa aagcggcgta acgctataaa gtcgaaatga
     7981 gttgttaata aaaaacggcg tttcataatg tgaagcgccg tttgcttttc catacctgct
     8041 aaggatttat catgaccgcg tattggctgg cccagggcgt gggtgtcatc gcctttctga
     8101 ttggtatcac aacatttttc aatcgtgacg aacgtcgctt caaaaagcag ctttcggtct
     8161 atagcgccgt tattggcgta catttttttc ttctgggcac ctatcccgct ggtgccagtg
     8221 ccatccttaa tgccattcgt acattgatta ccttacgcac gcgcagctta tgggtaatgg
     8281 cgatttttat tgtgctgact ggcggaattg gcctcgcgaa gttccatcat cctgtcgaac
     8341 tattgccggt tatcggtacg attgtcagta cctgggcgct gttccgctgt aaagggctca
     8401 ccatgcgctg cgtgatgtgg ttttcaacgt gttgctgggt gattcacaac ttctgggcgg
     8461 ggtcgatagg cggcacgatg attgagggta gttttctgct tatgaatggc ctgaatatca
     8521 ttcgtttctg gcggatgcag aaaaggggaa ttgatccgtt taaagtagag aaaacccctc
     8581 ccgccataga cgaaaggggt taaacaatta attacgcagg gcgctatttg ccagacgatc
     8641 gtcttccgcc tggcaagctg ccgcagtgaa cagcacgtca gttgaagagt tcagcgcggt
     8701 ttcgcaagag tcctgcaata cgccgataat aaagccgacg gcaaccacct gcatggcgat
     8761 atcgttcgaa ataccgaaca tattacaggc cagtgggatc agcagcaaag aaccccccgc
     8821 cacgccggat gcgccacagg cacacagaga agccactacg ctcaacagca gcgctgtggg
     8881 cagatcgacc ggaataccca gcgtattaac cgcagccagc gtcaacacgg taatggtgat
     8941 tgctgcgccc gccatattga tggtggctcc cagcggaata gaaactgaat aggtatcgcg
     9001 atccagattc agcttttcac acagcgccat attcaccgga atgttcgctg cagagctgcg
     9061 ggtgaagaag gcatacacac cgctttcgcg caggcacagc agcaccagcg ggaacgggtt
     9121 acgacgaatt ttccaccaca ccagcaatgg gttaaccacc agcgccacca gtaacataca
     9181 gccaaccagc acgaccagca gttgcgcgta gccccacagt gtggagaaac cggtggttgc
     9241 cagggtagaa gaaaccagcc caaaaatacc gaacggtgcg aagcgaatga ccagtttcac
     9301 cataaaggta acggcattcg acatatcatt gaccaggttt ttggtggtct cgttaccgtg
     9361 acgcagtgcg aagccgaggc cgatcgccca caccagaatc ccgatgtagt tacctttcag
     9421 cagggcgtcg atggggttgg aaaccatgct cattaccagt ccgcgcatca cttcgacaat
     9481 gcctgacggc ggcgaaatat cacccgcgct actggacaag tgcagggtag aagggaaggc
     9541 aaagctgaag actactgcgg ccagagctgc agagaaggtg cccagcagat agaggaacaa
     9601 aataggacgg atattggttt tctgcccgtg ctggtggtta gcaatagatg ccattaccag
     9661 catcaacacc aggatggggg caacagcttt cagtgcgccg acgaacaaag tacctaacag
     9721 accaacagct tccgccgcgg gttttgagat ccatgccaga agaatcccca gaacaaggcc
     9781 gaccaggatt tgttttacca ggctgccatg agccagacgc cggaatagcc ccggtgaacg
     9841 ttgcgtagtc atttttcgat cctttcattg tgttgtcgca catccctggt gcgttctgtc
     9901 gacgcatctt tcaggatgta acaaagtgtt tgcaaggtcg agtataagga aagttacagc
     9961 ttcgggaagc gaaaaatgct ggattttaaa gagaggtcat gttttgcaac ttaatggttg
    10021 catttattta acataaacag agggaaggac tggctactaa ggctgattag ataacatatg
    10081 ggcaaaacta tttggaatat gagaacggtt gcgctccttt aacgtgttat gccatataaa
    10141 gtcactggcg cagggagcgc gcagggggcg gccaatcgcc gccgccccct gcacccccag
    10201 gctctggcga acaaaatcgc cgctgcgcgg ttccctcagc ctatcccttg cggctaccgg
    10261 gtcgggcgca aggtaacgtc cctgtaagat gcgccctcgc cccggcctcc agggaacgct
    10321 tcggcgattt tgacgccacc aacacccgtg cggtcttttt gtaaagagct ataagccgat
    10381 tacataatta atagctcacc tttgtctgga atgagttatc tctaggaatg gcttaatata
    10441 cagcgaatca ttctccccgc tgcttatcat gccgataatt cacccaggcg ttgataataa
    10501 acgtcatcac cagaatgcca aacaccacgc ccagcgagac ggcgattgga atatggtaga
    10561 agtcgacaat cagcatcttg ataccgataa acaccagaat caccgccagg ccatatttga
    10621 gcatcgagaa acgctctgct acgcccgcca gcaggaaata catcgcacgc aggccgagga
    10681 ttgcaaacag gtttgaggtc agcacaatga acgggtcagt ggtcacggcg aagatagccg
    10741 gaatgctatc cacggcaaaa atcacgtcgc tcaattccac cagaatcagt accagcatca
    10801 gcggtgtggc gtacagcaat ccattcttac gcacaaagaa atgctcgttg tcgatggtgt
    10861 cggtcatgcg caaatgaccg cgtagccagc gcaccagcgg cttgtcgcca atgcctgatt
    10921 catcttcatg ggcgagcgcc atcttcacgc cggtaaacag caggaaggca ccgaagatat
    10981 acaggatcca gtcgaactgc gaaatcagcc agctgcctgt gaagatcatg atggtacgca
    11041 gaacaatcgc cccgagcacg ccatacacca gcacgcgacg ttgtaatgcc gccggaacgg
    11101 agaaatagct gaacaacatc agccagacaa agacgttatc gaccgccagc gatttctcaa
    11161 tcagataacc cgtgagaaag gccagggcct gtggatcggc aacggcgcga ccttcggttt
    11221 gcaccagata ccaccagaaa gcggcgttaa acagtaacga cagcgtcacc cagaccagcg
    11281 accaggccgc agcctgtttc atggtcatgg catgtgcccc acgacgcccc tgcaacaaca
    11341 ggtcgatagc cagcataatg gcgacaacaa cagcgaatcc gccccatagc aacggcgtgc
    11401 cgacagtatt catagaagtt ccttacacat aaaaaaacgg ccaacgtcgg aagacgtcag
    11461 ccgctgcttt ttatgcatag acctcgcctt ccggcaaggt ctcacttaca acaaaaaagg
    11521 aatacgtctg cgtattcctt tcgggttgcc cggtgaccgg atgtggtttt tcactcatcg
    11581 taatgacgat cgaccggcaa tgaagttact cccctttgca ggtaacaaag tatgacagac
    11641 cattcagtca gtcaatggtc tgttacatac cttttacact gttttacttt ggcaagtata
    11701 gttttacgct atctgccgga aaaatcaccc cagtttggcg gcggatctcg gtcagcagtt
    11761 tggctgtgat gcgactgacc gccagccctg gatgatccac cagatgctca tccaccaggg
    11821 tagcgaacag ctctgcttcg tagagcatgg tattaatatg ctgcggctgg gtgagatcct
    11881 gcatctggct gccacgcggc acaaagcaga ctttctggca ttcagacagt ttttcaatca
    11941 ccagcgatcc cgcttcgccc tgaatctcgc ttcccaggac agaatcactg acctttgagt
    12001 gctgcaaggt gacgctgaaa tcaccgtaat ccatcaccac cacgccgtgg gcgtcaacgc
    12061 cgcttgccag caaactcgcc gttgcctgca cgcttttcgg ctcaccaaat aacgccaccg
    12121 ccgacgccag gcagtaaaag ccgatatcca taattgaacc gttagagaat gccggattaa
    12181 aggtgttggg attctcaccg tccaggtaac gttgataccg cgaggaatac tggcaatagt
    12241 tgaaaaagac tttacgcagt ttgcctgctt tcggcagcgc ctggcgcaac aaatgaaagt
    12301 tcggcaggca ggcggtttta aatgcctcaa acagcaccac ctgattttcg cgcgcacagg
    12361 caatggcggc atccacttcc gccagattcg acgccagcgg tttttcgcaa atcacatgaa
    12421 ttttatggct aaggaaaagt tgtgtctggg aaaagtgcag ggaattcggg ctggcaatat
    12481 acaccgcgtc aatggcatcg ctttccgcca tcgcttccag cgacgtaaac agatgctcga
    12541 cagaaaaatc attggcgaag tgctgagcct gctcaaggct gcgggaatat acggcggtta
    12601 acttgtattt accgctctca tgggcggcct cgacgaactg gcgagtgatc cagttcgtac
    12661 caatcacagc gaaacgtatc ataacgcgtg gatactccat aaagggattc tgtcgtcaat
    12721 gtagcacgcc tttttcatct tcgacgtgcg cagatacgca ttattctttc gccggacagc
    12781 catgctcact aaacggccca atcatgaccg atggcccgag atatttcggc tggcgatgga
    12841 ggatttttgc gtcaatcacc gcgctgacac gagcgagttt ctcccgcagt tgagtataaa
    12901 aaccgtgctt catattgagg tctggcgcgt taaacccgat agcgtcgatg ccgtactgtt
    12961 ttgccagcca gatggtgcgt tggttatgaa attcttgcga gataatggtg atatggtttt
    13021 ccccaaacac ctttttggca cgcactaccg aatccagcgt tgagaatccg gcatagtcgc
    13081 aaaagatcac tttcgccggt acgcctttag cgatcaaagc ctgctgcata cctgatgctt
    13141 catcgtaatt tttacgtccg ttatcgccac tcaccagtag ccatttcact tttccggcgt
    13201 ggtacaacgc tgctgcggta tcaatacgtc gggtaaagta gcgattaccc ggcctcgccc
    13261 ccagcaataa acccacgtta cgcgccggga cggcgttgac atcgccccag gtcagctgct
    13321 tgctggcgtt gaccataacg cgatcggcga taaaaatggt cgctcctgcc acaagtaaca
    13381 gtaagcaggc aatctttaga gtccggcgag aaaaacaaat acggagaaga aggcgggcaa
    13441 atgcgcgcag catggtcgtt atccttaacg aaagggggga ggtcgatttt aggcgctaaa
    13501 gaaagaaaag ataacgcctg acatcatttt cagattttgt cctgcccaag cgacaactgc
    13561 gttagccaca gccgggtgtc gaactcaagc tggtggtact gcggctccat atggcagcac
    13621 aattgataga acgctttgtt atgctctttc tccttcaggt gcgccagctc gtgcaccacg
    13681 atcatgcgca aaaacggttc cggcgcgttg cgaaacacgg tagcgacgcg gatctctgct
    13741 tttgctttca gcttgctgcc ctgcacacga gaaacagcgg tatgtaaccc gagcgcgttt
    13801 tttagcacat ggatcttatt gtcatacatc actttattga tcggcggggc attacgcaga
    13861 aactgatttt tcagatcctg agtatattgc cagagagctt tatcggtggc gtagtcgtgt
    13921 gttcccggat agcgttttgc cagcacatcg cccagacgct gttcgttaat cagtgttcgc
    13981 acctgcgaaa gcagctgctc gggataaccc tgaagataag taagattgct cataaccgcc
    14041 cacgaaaaag agaaaaaggg tatactcacg cacccttttc aggggcaacg tcgaaatttt
    14101 atcattcagg agggccgatg agccacttag acaacggttt ccgttcactg acactacaac
    14161 gttttccggc gacggatgac gttaacccgc tacaggcgtg ggaagcggca gatgaatatt
    14221 tgctgcaaca gttggacgac acagaaatcc tcggcccggt gttgatcctg aatgatgcct
    14281 ttggtgcgtt aagctgcgcg ctggcggaac ataagccgta cagcattggc gactcataca
    14341 tcagtgaact ggcgacgcgc gagaatttac gcctaaacgg gattgatgag tcgagcgtga
    14401 agtttctcga cagcaccgcc gactacccgc aacagccggg tgtggtgctg atcaaagtgc
    14461 cgaaaacact ggcattgctg gaacagcaac tgcgtgcgct gcgcaaagtg gtcacgtcgg
    14521 atacacgtat tattgccggt gccaaagccc gtgacattca cacttccacg ctggaactgt
    14581 tcgaaaaagt gctcggcccg accaccacca cgctggcatg gaaaaaagcg cgcctgatta
    14641 attgcacgtt caatgaaccg ccgctggccg atgcgccgca gaccgttagc tggaagctgg
    14701 aaggtactga ctggactatc cataaccatg cgaatgtctt ctcccgcacc gggcttgata
    14761 tcggcgcgcg cttctttatg caacatctgc cagagaatct cgaaggggag attgtcgatc
    14821 tcggttgcgg taatggcgtt attggtctga cgctgcttga taaaaacccg caggcgaaag
    14881 tggtgtttgt cgatgaatcg ccgatggcgg ttgcttccag ccgtttgaac gttgaaacca
    14941 acatgccaga ggcgttggat cgcagcgagt ttatgatcaa caacgcgctc tccggcgtgg
    15001 agcctttccg ctttaatgct gtgctctgca accctccgtt tcaccaacaa catgcgctga
    15061 ccgataacgt cgcctgggag atgttccact acgcccgccg ctgcctgaaa atcaacggcg
    15121 agctgtatat cgttgccaac cgccatctgg attacttcca taaactgaag aaaattttcg
    15181 gcaactgcac cactattgcg acgaataata aatttgtggt gctgaaagcg gtgaagctgg
    15241 gacgtcgtcg gtaagattgg gagttacctg ccggatgcgg cgtaaacgcc ttatccggcc
    15301 tacgttcagc acgttacccc caggccggat aagacgcggc aagcgtcgca tccggcaatg
    15361 tctgctcagt tctggaatcg cgcttccata aaacaaaaaa tccctgtcac catcctctac
    15421 attctcttgt ttagcgtttt tctacgttta ttcttccgtc acacagataa attcatccgt
    15481 tgccaatctg tcaacggatg ttatcatatt gccaatttgt caacggacgt gatgaagaat
    15541 gcacctgata actcaaaaag cattgaaaga tgctgcggaa aaatacccgc aacataaaac
    15601 ggagttggtg gctctgggga acacgattgc taagggatat ttcaaaaaac ctgagtcatt
    15661 aaaagcagta ttcccatctc tggataactt caaatatctg gataagcatt atgttttcaa
    15721 tgttgggggc aatgaattac gtgttgtagc aatggtcttt tttgaatcgc aaaagtgcta
    15781 catacgtgaa gttatgacgc ataaagaata cgatttcttt accgctgttc atcgtactaa
    15841 ggggaaaaaa tgattgcgat tgccgacatc ttgcaagcag gagaaaagct aactgctgtg
    15901 gcaccttttc tggcgggtat tcagaacgag gaacaataca cccaggcgct ggaactggta
    15961 gatcatctgc tgctcaacga tcctgaaaac cccttgctgg atctggtgtg tgccaaaata
    16021 accgcgtggg aagaatcagc gcccgaattt gcggaattta atgccatggc tcaagccatg
    16081 cctggcggta tagccgtgat tcgtaccctt atggatcaat atggtttaac cctttccgat
    16141 ctgccggaaa ttggcagtaa atctatggtg tcacgcgttt tgagcgggaa gaggaaatta
    16201 acgctggaac acgctaaaaa attggcaacg cgattcggca tttctcccgc cttgtttatt
    16261 gattaacgcg acgtgccgaa tgcgttgcat ccggcgcttc aaataacgat ttaaatctcc
    16321 agcgccagcc gtgttccctg ggcaattgcc cgtcgtgcgt ccagctccat agccacatcg
    16381 cagccgccaa ttaaatgcac agttttcccg ctatcaatca gcggttgcgc cagcgcgcgg
    16441 tttggctctt gccctgcgca gatcaccaca ttgtccactg ctaatacctg cgtttcgccg
    16501 ttgatcacca catgcagccc gtcatcgtca atcttctgat aacttacgcc tgggatcatt
    16561 ttcacacccc gcgagagcag ggtggtgcga tggatccagc cggtggtttt gcctaacccc
    16621 tgtcccggtt tgctggcttt gcgctggagc atcacaattt gccgtgggct acgcgggatc
    16681 tgcattccct gcgggcttaa gccaccagcc tgttgtaggc tgctgtcgat cccccactca
    16741 ttacagaacc tggcgatatt ctggctggtg gattcgcccg gctgacttaa atacatcgcc
    16801 gtatcaaaac caatcccgcc acaaccgatg atggcaacct tgttgcctac cggcgctttg
    16861 tcgcgcagta catcgagata actcaatacc ttcggatgat cgatcccgtc gataggcgga
    16921 acacgcggca cgatcccact ggcgaggatc gtttcatcga acgcctgtaa ctgatccgcc
    16981 gtcacggtgt gattgagttt cagcgtcacg cccgtcactt cgatcatccg gcggtagtag
    17041 cgcagcgttt cgtaaaactc ctctttgccg gggatctgtt tggcgatatt aaactgcccg
    17101 ccgatctcgc tgtgcgcatc aaacaatgtt acctgatgcc cacgcgccgc cgcgttaatg
    17161 gcaaacgcca gcccagcagg tccagcgccg accaccgcca gatttttttt ctgcacggcg
    17221 ggaaggattg gcattttggt ttcgtggcag gcgcgaggat tcaccaggca cgaggtgact
    17281 ttgccaacga agatctgatc gaggcagccc tggttgcagc caatacaagt gttgatctca
    17341 tcggctcgcc ctgattgcgc ttttgacagc agttccgcat ccgcgagaaa cggtcgcgcc
    17401 atcgatacta tatcggcatc gccgcgcgag agaatatcgt cggcaacctg cggatcgtta
    17461 atccggttgg tggttaccag cggcagcgag acgtggcctt tcagtttgcg cgtgacccag
    17521 ctaaatgcgc cgcgcggcac gggcgtggcg atggtcggaa tccgcgcttc gtgccagcct
    17581 attccggtgt tgataatggt cgcgcccgcc gcttcaatgg cctgcgccag ttctaccgtt
    17641 tcggcaaaag tgccgccgcc ttcgactagg tcgagcatcg acaaccggta aataataatg
    17701 aagtcgttgc cgacgcgttc gcgcaccgta cgcactactt ctacggcaaa tcgcatccgg
    17761 ttgcggtaat cgccgcccca ctggtcacta cgctgattgg tgcgcagcgt cagaaattcg
    17821 ttgatcaaat acccttcaga gcccatcact tcgacgccat cgtatccggc ctcccgcgcc
    17881 agttgcgcgc agcgggcgaa atcgtcgatc agttgcagga tctcttcatg ggtgagttcg
    17941 tgaggaacga aacggttgat gggggcctgc aatgcggacg gtgcgaccag atgcggttgg
    18001 tagctgtagc gcccggtatg caaaatttgc agggcgattt tgccgccttc ctgatgtacg
    18061 gcttcggtaa tggtgcgatg gtgtgggatc tggctggcgt cgttgagcat tgcgccacct
    18121 tccatgccaa cgcctgttaa atctggtgca atgccgccgc tgacaatcag cgccacgccg
    18181 tgacgggcgc gttcggcata aaacgcagcc agccgttcag caccgtccgg gtattcctcc
    18241 agcccggtgt gcattgaacc catcaacacg cggtttttta acgtggtaaa acctaaatcc
    18301 agcggggcga acagcgacgg gtagctcata aaattgtcca gtatgtaaaa taattgttat
    18361 gtagtcggat gagttctaat ttagccgtcg atggagaaaa gggaaaaggg gagtgcgggg
    18421 tttgtgatgg gattcaaaaa gaatacgccc ggtggtaacg ccggatgcga cgcttgccgt
    18481 gttttatccg gcttggggga ttgcacatgt agggcggata aggcgttcac gccgcatccg
    18541 ccagccgttg caaattactg cttacggaaa aaatcgttat acaacatata caaatgagcg
    18601 gcactccagg agaaatttgg tgcgccttgc tgcgcgcctg tcagcgggtt gtaattctcc
    18661 tgaattgggc catcggcggt taatcctttg gcgtgcctga agaacgtatc cgccagcttc
    18721 agggcatcat cacgataacc gtaacgctcc atccctttca gaccaaacca gaactgatcc
    18781 acccatacgc gcccgcgcca gtagatatca gcgccaaaag ccggattggt taacgccgcc
    18841 gttcccagcg gaacaaatgt gttgaactct ttaggatcga gcatcacctt caccaccgcg
    18901 tcggcattgg cctgcgttgc cgcaccgtta aacagcggcg accagccttc cggcccttta
    18961 ccgcgctcaa caatcggttt gcccgcgcag ccgttcgcca gtggtttatc ttcaatacgt
    19021 acgtcatagt agaactgggt agtcgggtcg aacatacagg tgttgatgta gtccgcgagc
    19081 tgctgtgcca actggcgata gcgtttggcc tcttctggct taccgaggat ggttgccatc
    19141 tccgccagat aatggttatc gctgtacata tagctggcct gatccaccga ctcctgcaat
    19201 agcgagtagc ccagcaacgt tccgtcctga ctgcggtttt cggcgaattt caccgtccag
    19261 tcgctacgtt tgccgccatt agcgacatat ttatccaact gttctttgtc gataaaacca
    19321 aagacggcgg catcatcacg ccccgattcc cacgacgcag cgacctgtgc cggaatttcc
    19381 agactgtcgt actggccttt ctccaccacg cgggcgtagt tgttcagccc agactgcgtc
    19441 tcttctttat cgcctttttt caccgtaaac agcatctcgc cgctctcggt gttgtgggct
    19501 ttgtcgcggg tcgcgccata ttccggcacc ccgttgccgt tatgatcgcg gttacgtaac
    19561 caccagtcgt gataggccac cagtttcggg tacatctctg ccaaccaggc tttatcctgg
    19621 gtgacgttgt atacttccat caccgaccag gcggcaaggc tgggtttggt attacgttcg
    19681 ttccagttgc cgccatcgcc accacgctcg gggctaaggt tccacgctat caggtcgggg
    19741 acaaagccca catcctgcgg acgcacgctg tcgccaggct ggatctgcca ggagaagacc
    19801 gcgcggatat tctctttggc gatatccgga ttgaaatgcg ccatcgcaaa cgcctgcttc
    19861 caggtatccc acggccaggt ctgattgccg gagaaccagc gtccggtcac cgacggcgtg
    19921 acggtgttat aattcaccgc accgcccggt gagcgccagt taccgttgag cgtttcgatc
    19981 gctttcaccg cgacgcgcgt ctgttccggc gtcgcatccg gattggttaa ccctttcttc
    20041 aggtattctt cccagcgttg ctgcgaggcg gtgagataaa acgccggacg cgccagaata
    20101 tcgcggatct gcatttgctc tttgctaact tcctgagcgg tcagcagatg ggaataggtg
    20161 gtgtagagcg tggtcgaacc gttgatatgc gccttactgg taaagcgatt accgttgatt
    20221 tcagtctgca ccggcaggga tttatgcacc tgatattccg attcgccgga ggtcagcaga
    20281 tcccaggtgg cgcgcacttt gccaaaggtg actttcaggc catcacgggt ggcgctgatt
    20341 ttgcgctgat agtcagggta ttcgccagca atggttttat cggaaagcgg tttcccttct
    20401 ttcgcttcca gtttttccag cagttcgcca tcccacacca gatccagcgg tttattgctg
    20461 atgattttag tttccagtag tgacgtgcgc ggcgtggcga agcgcagagt catttcgacc
    20521 tgcacatctt ttgctgtcag tttttgcacc agcgcgccgg gaatactgta tgcctccagc
    20581 gtgaagttga ctttcttgcc gtcttgccag acagtcaggc ggtcgaaatt gctggccata
    20641 aagttgatgt attcttccgt cagcagcgca acgcccggaa agccgcccat ggtgttcggg
    20701 ccgtctggca acagatgacc atgccaggct ccgagatcga aaaacggatt aaaacgctgg
    20761 tgatcgtcgt aatcgtaatc cttcatgtac tgcggcgcac cagtacggtt aatcacgttt
    20821 ttataattgt cggcgttagc ggcatgagcg gaaaaactta tcagcagagc gcaggttact
    20881 ggcgttaaaa tagttttgat tttcatgaga ttacagtcct tattaccagt agaaatattg
    20941 catcaggaag atttggtcgc tgggatcgtt agaggcattt ttcatgtaga agcggctatc
    21001 aaaagccgtg gcgaaacgtc cgccgccata ttcgtaccag ataccaaact gcatgaaaga
    21061 ggtggtttca tcgtcagcgt ggtcagggcg atgtttggcg taggtgtaag cggtggagag
    21121 ataaacgcct tttaacgctt catacatggc gctcaccatc atcccgcttt cacttcccgg
    21181 tacgtccagc ccgtcggcac gtccggtatg gtgccaggcg cgagcggcaa agttaggggt
    21241 aaacaggccc gtgagataga gttgtcctgt gccgtcggta atttccagac cattcatcca
    21301 ggtgatgcct tcggcaaggt catactggat gtaaccgttc atccattgcc ggataggtgt
    21361 atttgtcgtc gtagcgatcg tacttgccaa aatgtagcca ggctttgctt tcgtcatgat
    21421 gtccggcagg tgtggcggtg acgctataac gaaagttgcc gcttaagttc tgtaccttca
    21481 gcgcggtcat aatgtcgcgg gtattgggaa tgacgtagcc caaatcgggt gtgaagtcgc
    21541 cccaccattg caggtcatcc agcgaggaat cctgacgcag actgagcatg atttcggtgc
    21601 catcattggt acgataaccg ccatagaaac ggttaatccc accttcgaaa ccaccccagc
    21661 tatggtcagg cacctaggca ttaccgtcgt ggtcggcctg cacggtccag ccttcgccgt
    21721 aaatcaggcc gaaccaggag ccgtacttca tctccaggcc accttcaata taggtgccgt
    21781 cgctgtattt atgtttattc tcaccctcta aatccatata tccaccaagc cctaattcgc
    21841 cgtaaaaacg aaatgaagga gaagatgtcg aggtaattgc gggtgttttt gtgtcgtcat
    21901 gttctgccgg acgtgcttct tctcctgccg cagaaaaggc gtagcaaaac ggcagagcaa
    21961 gtaatgctct gcatggtgca gtgattaatt tcattgtgtt aatccatgag ctaaagaaaa
    22021 agtacagcct tttatcgttg taattataaa aatataaagg cagaaactat tttccctctc
    22081 ctcatccagg aagagggaaa atgcctgctc attttttatt agtctgctta atcaacattg
    22141 ctggctggcg tcgcttcaat atggcgctct tcagccgtta atccctttat atatttgctg
    22201 tatttccacc acgcaaagcc gaggaagata acaataccgc cgacgttata aaaaatgatg
    22261 gtcagaatat tcgcgccagt cgggaatgtc gaagcgacaa accctacggc aaatatcgca
    22321 atcagcattg aaacaacgat aataccggtg cggcgggagc ccatacggaa atcgcgtggc
    22381 aggtgatcta atttggcgcg taaattcagg taagccagca tgatgaataa cggcggaagc
    22441 atggacgctg cggcggtcat attaataatg gtattcatca gatcctgcac ggtattggaa
    22501 ccgagcatcg ggataatcat cagcgggatg acgatcagaa actggatcca tgctgcgcgc
    22561 gccggaacgc cgttttcgtt aagttcgacg gttttcttac caaagatgcc ttccgggatt
    22621 tcggagaaga aaattttcac cggcgttgcg gtccacatca gcagggaacc gaacattgcg
    22681 gtaaaggaca ccagaccgac aaagcgattc atcaacgctt ccggcagacc aaaatacgcc
    22741 gccatgccgt ggaatacctg caccgatccg ccggtaaatt tcaactcttt gctgctgacg
    22801 aagacgttaa tcagcaccga ggagacggaa tacagtacgc cgataaaaat cccggcgagg
    22861 atgatcactt taacgaacga tttcgaaccg cctttgacgt cgttaacgta caccgcgacg
    22921 gactccgcgc cacctgcggc cataaagatc caggtggtaa cgccgaggaa cgcccagttg
    22981 aagttcggga tcatcgcgtc aacggtgatc ggatcggcag gctgtacgcc gccaaccagt
    23041 gccgtacctg ccagtaaaat gtaggagagc gttaacagca gcatcagcgt tgaagtgacg
    23101 gaggtaattg gccccagcat tttcgccccg ttggtggaaa cccaggtgga gaaggcgaac
    23161 agcaccatac tgataacggt ggtggcaacc ggcgtcataa tatattcgta gccgaggaag
    23221 gcatacgaag cataagcaat aacgcgcggc aacagcgagg tgaaaaagaa cagatttacg
    23281 aaccagtaag tgtaggcagt aataaatgcc caacgtccgc ccagcgaact ttttacccac
    23341 gcgtagacac cggcttctga gtttttattt aacgaaacaa attctgcgat gatcagacag
    23401 aagggaataa aataaaaaat cgtcgcgagg aaaaacatcg gtgccgaggc cagtccaagc
    23461 tcaatattat tattgataac gttgttaaag ctgtaaacgg cggcaaaagt cagcgagagc
    23521 aagccgaatt tgccgattgt attacgtttg gtatcagaca taacacatct ccgaaaatgt
    23581 gtagggtagc ggtggcggca aactcctttt gccgccgtag ttgttatttg ttatggaaat
    23641 aaccatcttc gatggtgact ttgagaacca cttttttgac cgcgttattg cagataaacc
    23701 gatacgcttc atggatatcg caaataacga tttgcccttc gtggacctct acggtttctc
    23761 cgcagccttt taaatattca cggtcagttt catcacgata atattccact acctgtaatg
    23821 tctctttcgg cgcatattca attttttgct gcccttgcag gtagtaatgc acttcaaaat
    23881 agcgtcgatg cccggtaaat agcgcatcgg tcgcggagtc attttccaca cggtaggtca
    23941 atgaatcacc gatggagtgg gcgacgccag gctgaatgtt gtcgatattt tcaatcgctt
    24001 caacgcagcg ttgccacttc ttgccagagg cgtaaatctg gcggaactgt tctaagttat
    24061 cgatgatcct cattgcttat tctcgctgtg caaattcgtg gaaaagaacc ctgcgccgaa
    24121 ctcatacgac gccaggcttt gcgcggtagc ttctccgcca gaaaccggca gcaacgtaaa
    24181 gccgtagctg aagtcacgga accagacgcg ccaggagtcc agcacctcgc tgccccagga
    24241 gttggagccg aggccaagta gctggtggtc gaggttcagg gtgatgtcat cactgcgctg
    24301 cagctcgtta cagtgctggg cagcgtggat gttttcctgg gtatagtgcc aggcgctgaa
    24361 gttaattggg cgctgcggaa ccaccagcag accgttgccg tggcggttag tcagtgccgt
    24421 ccagcggaca tgctgacggt tgccgttgtt ctgcgggaag ggatagttct cgaacatggc
    24481 atcgacggtg ctgcgccaga tatcgatgat gttagcctgc tggctgtcgg cgtagttttc
    24541 gcccggtcca cgaccgtaat acgccacctg atcgtattcg ccgttaatcc ccatggtgaa
    24601 accgatgcac ggaatgatgt gcggatagtc gccgtaacgc tcgccggaaa gcgccacgtt
    24661 aacctggcca tcggcagcga tgcgccagat gtaggtacag cgcatcccga agtcaaacac
    24721 cggcggtgca ataactgtgc ggctgatgat caacacttca ccatcgctct gttctacggc
    24781 aaagtcgcgc agatgttcct gcatgatctg caaatgattc ggctgccaca acccttcgta
    24841 ctcctgcttg tggttgtcga tcatcggctt gaagaagttg atctttggct cgcgggtcag
    24901 cagcgactcg ccatttacct gccaggatgt cggtttgcca ctcatttttg agaaggtgat
    24961 cgcgaagttg tagccgcgaa cggtgcagct caaacgatcg tcttccagcg tcagcggacg
    25021 cgcattattt ggtgcgaaag gcactggctg cgcggtgttt tccttcagcg ggaactgata
    25081 agtggcgata gaatgcccgg cttcgctgta gcgggtgcgg gaatctttgg tcaccgtaat
    25141 gttgaggaac gcttcgcggg cgtccagctg cggcagcgtg atctgcaagg gggcttcgct
    25201 gttcggcgca acgtcgcgca gtttaatctg ctgcgtcgcg agcgtttcac cttcggcgcg
    25261 cacctctgcg tgcagggtgt agtcatcaag cgtggtaaac cacagtttat tttcgacttt
    25321 caactcgccg cgagtcagcg cgtggatttt taccggcgcg ataacctgtt tgtactcttt
    25381 caggcccgat cccggcgtct gatcggaata gatcaaacca tcaagacaga agttatagtt
    25441 gttgggatag tcgccgtagt cgccgccgaa tttataccag acattgccgt ggtcgtcctg
    25501 tgcctggatc ccgtggtcgc accactccca gacataatga ccctgaatgc aatcgtgctt
    25561 atagaagacg ttctggtact ccgtcagccc gcccggtccg ttccccatcg catgagcata
    25621 ttcacagatg atgcgcggct tcggatgcgg gtattcacca aactcattca tcagcggcac
    25681 gcgggtgtac atggtggaaa taatatcgac cacttcagca tcgcgatctt cttcgtaatg
    25741 aaccagtcgc gtgtcatcca gcgctttcgc cgcatggtac atcgcgcgga tgttacagcc
    25801 atagccggat tcattgccca gcgaccagat gatgatcgac ggatggtttt tctgtgcgtg
    25861 gatatggcga acaatgcgct cgacgtagac tttttcccac tgcggatcgt cggtaatacg
    25921 gctgatatcg ccgacattag caaagccgtg cgattcgacg tcggtttccg ccatcacaaa
    25981 caggccgtag atatcacaca gttcgtaaaa acgcggatcg ttcgggtagt gagcggtacg
    26041 cacggagttg atattgtgct gcttcatcaa ctggagatct ttctcgacgc gatccattcc
    26101 aacggcgcgg cctttgcgat gatcgttgtc gtgacggttg acgccgtgca gcatcacata
    26161 acggttattg atccagaaca gaccgtcgcg cactttgata tcacggaagc caacgcgttg
    26221 tggcaccact tccagaacgt tgccgttggc gtctttcagc gtcatgacca gatggtaaag
    26281 ataaggggat tctgctgacc attgctgcgg ctgttcgaca gtaaaagcaa agctggcgct
    26341 ggtcagtttt tcaattgcca aatgatcaat ggcgctgctg tgcaccacgc gttcgccatc
    26401 aaacagggta tattccagcg tcgtgacgac aggggaggcg gcgagatttt ccagcaccac
    26461 ttcgcaggaa agcgtggcat cgcaataggc ttcgtcaaag tcggtacgca cagtgaaatc
    26521 gttaatatgc gttaggtgtt ttccgaccag ataaacatcg cggaagatcc ccgccgacca
    26581 ccacatatcc tggtcttcca cgtaggtaga gtccgcccac tgcatcacgc gcacacacaa
    26641 caggttgtcg ccggttttaa ccatcgcgct gatgtcaaac tctgcggtca ggcgactgcc
    26701 cttgctgaaa cccacatact gaccgttaac gtagacttca aaataggttt cgacgccgtc
    26761 aaatttaatc agcgtctgtt taccctgcca gccgtcgctg agggtgaaaa tacgttgata
    26821 ggcaccggtt gggttatcgc tggggacaaa cggcacatcg atggggaacg gaaaaccttc
    26881 gtcggtatat tgcagtttgc cgtgaccttc catttgccac atggcgggga cggtaatatg
    26941 cccccagtca gccattaact cagaggtgaa ggcttctggt acttgcagcg gatggtcaaa
    27001 aaagtggaaa ttccactgac tgcttaaggg cagaaacagg ctgctggttt cgcgggcaaa
    27061 ggtacgcgct tgcgcaacag aatcatatga aaaaaagtac gcacgcggcg caagtcggtt
    27121 ttcgtgggtg agctgaatgt tttcccagcg attcataggg cctcccggta gagagaaatc
    27181 ggaaccattc atggtgatgg tagtttgtgt aattgctggg ggtttagtaa aatttttact
    27241 aaaaagcagc aggtaaatgc atttacttta actggatcac gaaaataaac ggcagcactg
    27301 tccggcaaaa atgattgccg aacagtgtgt aaggggattt aacgggtcgt gccgcgcagt
    27361 tttaatttgc tgggaacgaa gactagcagc ggcagcgcgc gaccatcgcg ggctttttca
    27421 tacaccaggt taacgccctg acttcccatc atctcggaat ggatgcgcac ggtggagagc
    27481 ggcggaaagg taaatcgcgc ggtggggata tcgttaacgc tgataagcga aatatcttgt
    27541 gggatgttca ggccacgttc atgaattgcc cgcagtacgc cgatagcaat ggaatcggaa
    27601 gcaacaaaca gtgccttcgg atagtcttcc cgcgccagca tttgttttgc cagttcataa
    27661 cccgacgaac tggaaaaacc gccgcgccag atatcctctt cgcgtaccac ttgtttcagt
    27721 cggccatatt ccgcaaaggc gacctcacga atatccgcct tgccaggctc atcttcaccg
    27781 ccaataaaac caatacgatt aacgccctgg ttgatataga agtcgatgat ttctttactg
    27841 atgcgtgcca gatcgatatc caccgcatcg taaccgctgc cgggttcgtg aaagtcgata
    27901 aaacagatat tgtcggtcaa cgcgctggca gcggcgcgca gggcgggcgt gggtttgccg
    27961 acaattaaaa taccggtgac gtttttaatg tctggtaatc cgttgtgttc ataacagttg
    28021 gtcagttcga tcgccagctt ttcgcactga gtttcaatgc cgtggcggat cgccagatag
    28081 taaggatcgt tgatctccag ctcctgctgg tagctgtaga tagccagaat atggtgttgg
    28141 ttgactgcgc ctgtctgtag tttacgggcg ctactggtct tgtactccag cttttcggcg
    28201 atctcgagaa tgcgatgttt cgtctcttct ttcacattca atgtcggatc gtcatttaag
    28261 accctggata ctgtcgccag ggatacgcca gcttcgattg cgatgtcttt taatgttgcc
    28321 atgtttacct tttttacttt ccctgatgtg cgcccccatt gtagaggaag tgcttatcgg
    28381 gtcacctgtt ttcagtaaat aaagcgcgtt tctttgacgc tgtaacggta acacagcggc
    28441 ttaggaatgt gatcacgcct gcattctcca tttttagcct acaggattga gaaaaacttt
    28501 tctatagttt agtaaaattt tactcgaagg gaggaagcaa tggaaaccgt ggcttacgct
    28561 gattttgcac gtctggaaat gcgcgtcgga aagattgtgg aagtgaaacg ccatgaaaac
    28621 gccgacaagc tgtacatcgt acaggttgat gtggggaaaa aacactgcaa accgtgacca
    28681 gcctggtgcc gtactacagc gaagaggagc tgatggggaa aacggtggtg gtcttatgca
    28741 atctgcaaaa agcgaaaatg cgtggtgaaa cgtcggaatg catgctgttg tgtgcggaaa
    28801 ccgatgacgg cagtgaaagc gtgttgttaa cgccggagcg gatgatgcct gcgggcgtac
    28861 gcgtcgtata aaaagatcgg atggcgacgt cgtatcgcca tccgatttga tattacgctt
    28921 cttcgacact tactcgcatg gccgccagcg ccttacgcgc cgctttgatc accagttcac
    28981 actgttcaat ggtcagtgtc agtggcggtt caatgcggat cgttttggcg ttattgagcg
    29041 ttccggccac cagtacgcgc tggcggaaca tctcgctggc aaagttatag ccgatttcgt
    29101 tatcaacaaa ctcaatcgcc atcaacatcc ctttaccacg cgcttcctgt accagatcgg
    29161 gatattcccg cgccagttga cggaaaccgt ccagcaacat atcgcctttt tgctcagcct
    29221 gagccggtaa gttctgctcc agcaacacat tgatggtcgc cagcgccgcc gcacaggcca
    29281 gcgggttgcc gccaaaggtg gtggtatgca ggaatgggtt gtcgaacaga actgaaaaca
    29341 cctcttcagt ggcgatggtc gcgccaatcg gcatcacgcc gccgccgagg gcttttgcca
    29401 gacacagaat gtccggctga acgttctcat gttcgcaggc aaacatcttg cccgtgcgcc
    29461 ccatgcccgt ttgtacttca tcgaggatca tcagtgcgcc gaactcatcg cacagcttac
    29521 gcacggcggt gagatagccc ggcggcggca gaattacgcc accttcgccc tgaatcggtt
    29581 cgaggatcac cgcagccaca tcatcaccgg tttttttgca ctcattaaga gccgtgcgca
    29641 tggcttcgat attgccaaac ggcacatgac ggaaccctgg cagcagtggc ataaacggtt
    29701 tgcggaaggt cgatttcgcc gtggccgaca gcgcgccaag tgatttaccg tggaacacgc
    29761 cgctggtggc aataaaagta aatttgccgc gcggcgactg ataagccttc gccagcttca
    29821 gcgccgcttc aacggactcg gtgccgctat tacagaagaa gctgtatttc agtttaccgg
    29881 gcgttagcgc agcaagggtt ttcgccagca tagcccgtaa cggatcgagt agctcctggc
    29941 tgtgcagtgg ttgtttcgca agttgattct gtacggcgga aaccacaact ggattacggt
    30001 gccccacgtt gaaaattcca aaacctccca ggcagtcgat aaactcctgt ccctgggtgt
    30061 cgacaagcgt atttaaacct cccgcttgcc actctacggc tccgtaatcc ccgccggcgg
    30121 taacagattt gcgatactct aaaaaccccg gattgacatg ctctttgaag tattcaatca
    30181 cctctcggtt aagtgctttc atctcctcat gatccagcgt tcgcttctca atgagattca
    30241 gggcgtgggc gctgcacgct aaagccgatg cgctcgaagg taacctgttc aaaatatgct
    30301 ccggaggctc gcgtatcaca tgataccgat ttaagtattg cagggattgc gccactccgg
    30361 ctgcgatcgc gaaaatcggg ataaacacaa atgcggttat catttgtgca atatttaatg
    30421 ctatccgtta acatttgttg ttattttgca cggtttttgt cgggtatgga tttgatgatt
    30481 gctgcgcaaa tgcacgtttt cagggcaata attgcctgat ttttgggcaa gataattaac
    30541 gttttcgtat agtaacagag tgaaagtatt ttaaatcaaa gaattagatt tgtgctttaa
    30601 tcgcaaattg cgatctaaat caaattaatc ggttaaagat aaccgcagcg gggccgacat
    30661 aaactctgac aagaagttaa caaccatata acctgcacag gacgcgaaca tgtcttctca
    30721 tccgtatgtc acccagcaaa ataccccgct ggcggacgat accactctga tgtccactac
    30781 cgatctgcaa agctatatca ctcatgctaa tgacactttt gtgcaggtga gcggctttac
    30841 cttgcaagag ttacaagggc agccgcacaa catggtgcgt cacccggata tgccaaaagc
    30901 ggcgtttgcg gatatgtggt tcaccctgaa aaaaggggag ccctggagcg gcatcgtgaa
    30961 aaatcgccgc aaaaatggcg accattattg ggtgcgggcc aatgcggtac cgatggtgcg
    31021 cgagggaaaa atcagtggct atatgtcgat tcgtacccgg gcgacggatg aagagattgc
    31081 ggcggtggag ccgctgtaca aagcgttgaa ctccggacgt accagtaagc gtattcataa
    31141 aggcctggtg gtgcgtaaag gctggctggg taaactgcct tcattaccgc ttcgctggcg
    31201 gacgcgtgga gtgatgaccc tgatgtttat cttgctggcg gccatgcttt ggtttgttgc
    31261 tgccccggtg gtgacgtatt tcctctgtgt gttagtggta ttgttggcaa gcgcttgttt
    31321 tgaatggcag attgtgcgcc cgatagaaaa tgtcgcccgt caggcactga aggtggcgac
    31381 cggagagcgt aatagtgttg agcacctgaa tcgcagcgat gagctggggc tgacattacg
    31441 cgcggtaggg tagcttggcc tgatgtgccg ttggctaatt aacgatgtct caagccaggt
    31501 gtccagtgtc agaaatggca gtgagacgct ggcgaaaggc accgatgaac tgaacgaaca
    31561 tacccagcag acagttgata acgttcagca aacggtggcg accatgaacc aaatggcggc
    31621 gtcggtgaaa cagaactctg ccacggcgtc ggctgccgat aaactttcta tcaccgccag
    31681 taatgcggca gtgcagggtg gggaggcgat gaccacggtg atcaagacaa tggacgatat
    31741 cgccgacagt acctagcgca ttggcaccat tacttcgctg attaacgata ttgcgtttca
    31801 gaccaatatt ctggccctga atgcggcggt ggaagcggcg cgtgccggcg aacagggcaa
    31861 aggttttgca gtggtggcag gggaagtgcg tcatttagcc agccgcagcg ctaatgctgc
    31921 caacgatatt cgcaagctga ttgatgccag tgctgataag gtgcaatccg gttcgcagca
    31981 ggtacacgcc gccggacgga cgatggaaga tattgtggca caggtgaaaa acgtcaccca
    32041 gttgatcgcc cagattagcc attcaacgct ggaacaggcc gatgggcttt ccagcctgac
    32101 ccgtgcagtg gatgagctta acctgatcac ccagaaaaat gccgagctgg tggaagagag
    32161 tgcgcaggtg tcggcgatgg tgaaacaccg cgccagccga ctggaagacg cggtgacggt
    32221 gctgcattaa tcggtatgcc ggatccggca attgcaccgc gccactggcg gatgcggcga
    32281 ttaatccagt tgcgtaatat caaaagcggc gcggtcgatc accgcaatga tcttttttat
    32341 ttgtgcatca ctgatatcgc tctggttgac gcgtaaatcc agcaccgctt taaaattatc
    32401 cagcgcccgc ttcatttgcg ggttctggcg cagcgccgcg ccaacgcaac gcgctttgat
    32461 gcgttcttca atcatctcca cttgttcgcg gttttcttcc agccactgcg cgccttgttc
    32521 ggtcagcgca atctgcttct taccaccttc ctcttcgcgg atggtaatca gcgactgctc
    32581 ctgcagaaaa tccagcgtcg ggtagatgac gcccgggctt ggggtgtaat tcccctgggt
    32641 tagattctca atcgctttaa tcaattcgta accgtggctg tcatcgcgcg agagaatatc
    32701 cagaatcacc agacgcaatt caccgtgacc aaagaaacgt tgccgacgac cgccgccgcg
    32761 accgtggcga ccaccgcaac attgaccgtg ttcatgctgg tgaccgtgtc cgcagtgctc
    32821 gtgttctgac ttctcacctt tgcagcagcc ctcatggcgt ggctggcctt catgtttaca
    32881 acacccttcg tgatgatggc tcatatcagc ctccttttgc ttgattagat atatctaatt
    32941 tatatctgat gtgagcatta ctgcaagtac aaaaatgatt tttaaacgtg gaagcacctc
    33001 acatctaggc cggataagac gttcacgccg catccggcag cattgctatg gcactgaatg
    33061 ccttcacata cttcagacat atcagttcta caaatcgctt gcatttatca tgattaacaa
    33121 tcattatcat ttgcgaattt tatttagata tatctgatta ccatcacgaa ggcgataaca
    33181 atgaataaca ccccccgcta cccgcagcgc gttcgcaatg atctgcgctt ccgtgaactg
    33241 actgtgctac gcgttgagcg catcagcgcc ggttttcagc gcattgtcct tggcggcgag
    33301 gcgttggacg gttttacatc gcgcggcttt gacgatcaca gcaaactctt ctttcctcaa
    33361 cctgacgctc actttgtgcc gccaacggta acggaagaga gcatcgtctg gccggaagga
    33421 ccacgcccac cgtcgcgtga ctatacgcca ctgtatgacg aactacgcca tgaactggcg
    33481 atcgatttct ttattcacga cggtggggtc gccagcggct gggcgataaa cttacggtgg
    33541 caggtccgcg cggttcgctg gtggtgccgg aagattacgc gtatcagctg tatgtctgcg
    33601 atgaatccgg aatgcccgca ctgcgccgcc gcctggaaac gttgagcaaa cttgccgtta
    33661 aaccgcaagt tagtgcgctg gttagcgtgc gggataacgc ctgtcaggat tatctcgcgc
    33721 accttgatgg ttttaatatc gaatggctgg cacatgatga gcaggcggta gatgcgcgtc
    33781 tggcgcagat gcaaatccct gccgatgatt acttcatctg gataaccggc gaaggtaaag
    33841 tcgttaagaa tttaagccgc cgctttgaag cggaacagta tgacccacag cgggtacgtg
    33901 cagcggctta ctggcacgca aagtaactga tatataaaaa gaaaaaggct gacgatttct
    33961 cgtcagcctt tgctatatct ggtggcccct gctggacttg aaccagcgac caagcgatta
    34021 tgagtcgcct gctctaacca ctgagctaag gggccatggt agcggattat aaagtaactc
    34081 cgtgccgcaa tccagccatt accgcgcgcc tgctgttttt ataaacaatg tattttcaat
    34141 ccgttatact tttcctggtg atgttagaaa ggagtaaata tggttgagga tattttggct
    34201 ccagggttac gggtcgtgtt ttgcggtatc aaccctgggc tttcatccgc cgggactggt
    34261 tttccctttg ctcatccggc aaatcgcttc tggaaggtga tatatcaggc cgggtttacc
    34321 gaccgtcagt tgaagccgca ggaggcacag catctgctgg attatcgttg tggcatcacc
    34381 aaactggtag accgtccaac ggtgcaagcc aatgaagttt caaagcagga gctgcacgca
    34441 ggtgggcgta agctgattga aaaaattgaa gattatcagc cgcaggcgtt ggctattctg
    34501 ggcaaacaag catatgaaca gggattcagc tagcgcggtg cacagtgggg gaaacaaacg
    34561 ctcaccattg gttcgacgca gatttgggtg ctgccaaatc ccagcggttt aagtcgcgtt
    34621 tcactggaga aactggttga agcgtatcgc gagctggacc aggcgctggt agtgcgtggg
    34681 cgataaaaaa cgtcaccgg
//
LOCUS       DAEBXN010000035        34446 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.035, whole genome shotgun sequence.
ACCESSION   DAEBXN010000035 DAEBXN010000000
VERSION     DAEBXN010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34446
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.035"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(<1..331)
                     /locus_tag="KJL99_001742"
     CDS             complement(<1..331)
                     /locus_tag="KJL99_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4436493.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQ"
     gene            701..1651
                     /gene="corA"
                     /locus_tag="KJL99_001743"
     CDS             701..1651
                     /gene="corA"
                     /locus_tag="KJL99_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HBD4436494.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1692..2072)
                     /locus_tag="KJL99_001744"
     CDS             complement(1692..2072)
                     /locus_tag="KJL99_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HBD4436495.1"
                     /translation="MSKEYMNDDSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(2086..2502)
                     /locus_tag="KJL99_001745"
     CDS             complement(2086..2502)
                     /locus_tag="KJL99_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4436496.1"
                     /translation="MLTGDSHKDVKFMLRMFIPTSNGKISRHRYIFLFILINFIFAFL
                     IIFFNDGEAGFLVIVSTIALHYLVINMNCQRLRDSGFIYIKIYVFGTLAVYIISIITM
                     IAEDFACSGNGSMIFLICYFSTFSMLMLAPTDSSKQ"
     gene            complement(2561..3451)
                     /gene="rarD"
                     /locus_tag="KJL99_001746"
     CDS             complement(2561..3451)
                     /gene="rarD"
                     /locus_tag="KJL99_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HBD4436497.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(3503..3970)
                     /locus_tag="KJL99_001747"
     CDS             complement(3503..3970)
                     /locus_tag="KJL99_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HBD4436498.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            4135..5004
                     /gene="pldA"
                     /locus_tag="KJL99_001748"
     CDS             4135..5004
                     /gene="pldA"
                     /locus_tag="KJL99_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HBD4436499.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            5137..6966
                     /gene="recQ"
                     /locus_tag="KJL99_001749"
     CDS             5137..6966
                     /gene="recQ"
                     /locus_tag="KJL99_001749"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HBD4436500.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSRGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            7030..7650
                     /gene="rhtC"
                     /locus_tag="KJL99_001750"
     CDS             7030..7650
                     /gene="rhtC"
                     /locus_tag="KJL99_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HBD4436501.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(7712..8332)
                     /gene="rhtB"
                     /locus_tag="KJL99_001751"
     CDS             complement(7712..8332)
                     /gene="rhtB"
                     /locus_tag="KJL99_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HBD4436502.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            8443..9465
                     /gene="pldB"
                     /locus_tag="KJL99_001752"
     CDS             8443..9465
                     /gene="pldB"
                     /locus_tag="KJL99_001752"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HBD4436503.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATQTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            9473..10273
                     /gene="yigL"
                     /locus_tag="KJL99_001753"
     CDS             9473..10273
                     /gene="yigL"
                     /locus_tag="KJL99_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HBD4436504.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGISKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            10349..11248
                     /gene="bioP"
                     /locus_tag="KJL99_001754"
     CDS             10349..11248
                     /gene="bioP"
                     /locus_tag="KJL99_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HBD4436505.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRSNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQYNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(11136..12089)
                     /gene="metR"
                     /locus_tag="KJL99_001755"
     CDS             complement(11136..12089)
                     /gene="metR"
                     /locus_tag="KJL99_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HBD4436506.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALGNFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            12325..14586
                     /gene="metE"
                     /locus_tag="KJL99_001756"
     CDS             12325..14586
                     /gene="metE"
                     /locus_tag="KJL99_001756"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HBD4436507.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALSHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDNVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(14625..15440)
                     /locus_tag="KJL99_001757"
     CDS             complement(14625..15440)
                     /locus_tag="KJL99_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HBD4436508.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            15702..16463
                     /gene="udp"
                     /locus_tag="KJL99_001758"
     CDS             15702..16463
                     /gene="udp"
                     /locus_tag="KJL99_001758"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HBD4436509.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTVSSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            16604..18031
                     /gene="rmuC"
                     /locus_tag="KJL99_001759"
     CDS             16604..18031
                     /gene="rmuC"
                     /locus_tag="KJL99_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HBD4436510.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            18126..18881
                     /gene="ubiE"
                     /locus_tag="KJL99_001760"
     CDS             18126..18881
                     /gene="ubiE"
                     /locus_tag="KJL99_001760"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HBD4436511.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            18895..19500
                     /gene="ubiJ"
                     /locus_tag="KJL99_001761"
     CDS             18895..19500
                     /gene="ubiJ"
                     /locus_tag="KJL99_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HBD4436512.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            19497..21137
                     /gene="ubiB"
                     /locus_tag="KJL99_001762"
     CDS             19497..21137
                     /gene="ubiB"
                     /locus_tag="KJL99_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HBD4436513.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            21216..21485
                     /gene="tatA"
                     /locus_tag="KJL99_001763"
     CDS             21216..21485
                     /gene="tatA"
                     /locus_tag="KJL99_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HBD4436514.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            21489..22004
                     /gene="tatB"
                     /locus_tag="KJL99_001764"
     CDS             21489..22004
                     /gene="tatB"
                     /locus_tag="KJL99_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HBD4436515.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            22007..22783
                     /gene="tatC"
                     /locus_tag="KJL99_001765"
     CDS             22007..22783
                     /gene="tatC"
                     /locus_tag="KJL99_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HBD4436516.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSSEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            22825..23607
                     /gene="tatD"
                     /locus_tag="KJL99_001766"
     CDS             22825..23607
                     /gene="tatD"
                     /locus_tag="KJL99_001766"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HBD4436517.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(23604..24092)
                     /gene="rfaH"
                     /locus_tag="KJL99_001767"
     CDS             complement(23604..24092)
                     /gene="rfaH"
                     /locus_tag="KJL99_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HBD4436518.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTISATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPAPPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            24259..25752
                     /gene="ubiD"
                     /locus_tag="KJL99_001768"
     CDS             24259..25752
                     /gene="ubiD"
                     /locus_tag="KJL99_001768"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HBD4436519.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IDPGEMAPEGPYGDHTGYYNEVDNFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            25798..26499
                     /gene="fre"
                     /locus_tag="KJL99_001769"
     CDS             25798..26499
                     /gene="fre"
                     /locus_tag="KJL99_001769"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HBD4436520.1"
                     /translation="MTTLSCKVTSVAAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDLPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHL
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(26691..27854)
                     /gene="fadA"
                     /locus_tag="KJL99_001770"
     CDS             complement(26691..27854)
                     /gene="fadA"
                     /locus_tag="KJL99_001770"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HBD4436521.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDTQACLVGGVEHIGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(27864..30053)
                     /gene="fadB"
                     /locus_tag="KJL99_001771"
     CDS             complement(27864..30053)
                     /gene="fadB"
                     /locus_tag="KJL99_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HBD4436522.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     QEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            30243..31574
                     /gene="pepQ"
                     /locus_tag="KJL99_001772"
     CDS             30243..31574
                     /gene="pepQ"
                     /locus_tag="KJL99_001772"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HBD4436523.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRYTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            31574..32188
                     /locus_tag="KJL99_001773"
     CDS             31574..32188
                     /locus_tag="KJL99_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HBD4436524.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            32227..33678
                     /gene="trkH"
                     /locus_tag="KJL99_001774"
     CDS             32227..33678
                     /gene="trkH"
                     /locus_tag="KJL99_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HBD4436525.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            33690..34235
                     /gene="hemG"
                     /locus_tag="KJL99_001775"
     CDS             33690..34235
                     /gene="hemG"
                     /locus_tag="KJL99_001775"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HBD4436526.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMLKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         8108 a         8700 c         9303 g         8335 t
ORIGIN      
        1 tctgctgacc gttttcgatg tacaaaccga gcggcgcata gctttcatca tagatgccgc
       61 cgttcatcgc catctgcacc tgaccctgac tattaatatc cgccagcaga gcatgtaacg
      121 ttccccacgc ttcgccattg gctttttgcc agtacatttt cacccgctct gtttgaggat
      181 taacggtata cgcctgtacg gtcagcgtcg gatctgagag tgcgcaatca tcagcggcaa
      241 cagcaaacaa gggaagtaag gtgagggtga gaaaaatccg tttgagattc aaggtgatca
      301 ttcctttacc aatgagtagc cgatgcgcca ttataggtcc tagatgtggg atttttttat
      361 cctgttagcg accttgacga gtaccaaaaa gcgcgaagtt caactattgt tctgtggtgt
      421 tctgttgcgt gttgacggca aaattttgct ggcgtaacat gcgcgcacga tcactctaag
      481 aggacattcg ccttggacac acccagtaga tactggctca ctatcctgtc atccaggatc
      541 aactcctaag gctatccctt tttgctgata gccttagcgg ttgtcagcga cctcaatttt
      601 tcccgtcgcg ctgagtcagg ctgtttaatg gtctgaaacc caatttgttt ctgtgtgccc
      661 accgaactgt ccgatatttt aagcattggg agtcccggtc atgctgagcg catttcaact
      721 ggaaaataac cgactgaccc ggctggaagt cgaagagtca caaccccttg taaatgcagt
      781 atggattgat cttgtcgaac cggacgacga cgagcgactg cgcgtacaat ctgaacttgg
      841 ccagagcctg gcaacccgcc cggaactgga agacatcgaa gcatcggcac gtttctttga
      901 agacgacgac ggcctgcata ttcactcctt cttcttcttt gaagatgcgg aagatcacgc
      961 cggtaactcc actgtggcat ttaccatccg tgatggtcgt ctgtttactc tgcgtgagcg
     1021 tgaactgccc gcttttcgtc tgtatcgtat gcgtgcccgt agccagtcga tggtagacgg
     1081 taacgcctac gagttgctgc tggatctgtt cgaaaccaaa atcgaacagt tggcagatga
     1141 aattgaaaat atctatagcg acctggagca gttgagccgg gtgattatgg aagggcatca
     1201 gggcgatgag tacgacgagg cgctctccac gctggcggaa ctggaagata tcggctggaa
     1261 agttcgcctg tgtctgatgg atacccagcg cgctctcaac ttcctggtgc gtaaagcgcg
     1321 tttaccgggt gggcaactgg agcaggcgcg tgaaatcctg cgcgatatcg aatccctgct
     1381 gccgcataac gaatccctgt tccagaaggt gaacttcctg atgcaggcgg caatgggttt
     1441 tatcaacatc gagcagaacc gcatcatcaa aattttctcg gtggtatccg tcgtgttcct
     1501 gccgccaacg ctggttgctt ccagctacgg gatgaacttt gagtttatgc cagaactgaa
     1561 gtggagtttc ggctaccctg gcgcgattat ctttatgatc ctcgcgggcc tggcaccgta
     1621 tctgtacttt aagcggaaga actggttgta aaaagaaggc ggtggcttag cctggctaag
     1681 ccacctgtta ttcaaaggct ccaggtattt aaccctttta cctctttctc ataaaaccat
     1741 ttgttcgtgt taacagcaac ataggctgct acggcaattc ccagaatgtt aacgccaatt
     1801 aatgcaccaa caaatagaga tagaatgcca agtaatagaa ctataattgc ttttttccat
     1861 aagcccagaa caaataaata gatccaggaa cagaagaaag caatgaaatt catttgaata
     1921 gttaatcgtt gtcttacttt taatgcttta aatgctgctt tatattctgg tgttgctccc
     1981 cagaatccag gaaagccatg ttgatcataa aaattaaatc ggtatttcca tttttcactt
     2041 aatgaatcat cgttcatata ttccttactc ataaatactc cataactatt gttttgatga
     2101 atcagtaggt gcaagcatta gcatactgaa agtggaaaaa taacaaatca aaaaaatcat
     2161 cgaaccattg cctgaacagg caaaatcttc ggctatcatt gtgatgatag agatgatata
     2221 tactgctaat gtaccaaaaa cataaatttt tatatatatg aaaccactat cacggagtcg
     2281 ttggcaattc atgttgatga cgagataatg gagtgcgatg gtagagacta taacaagaaa
     2341 acctgcttcc ccatcgttaa aaaagataat aagaaaggca aaaatgaaat ttattaaaat
     2401 aaataaaaat atatatcggt gtctggaaat cttaccgtta gatgttggga taaacatacg
     2461 taacataaat tttacatcct tgtgtgagtc tcctgtcagc atggcaatat gcccactctc
     2521 atacaagagt cggcatattt tttcagaata tatttatttt ttatttggac gttctacgct
     2581 gcgtataaat cgcgtccatc acaaaaattg ccagcgccac ccaaataaag gtgaaagtca
     2641 ccatcttatc ggcacccggt ttttcaccat aaaacgtcac agccagcagg aacatcagcg
     2701 tcgggccaat gtactgaaaa aagcctaacg ttgagagacg caggcgcgtg gcagcggcag
     2761 taaaacacaa cagcggtacg gtagtgacaa taccggcggc gatcagcagt aaattcagcg
     2821 acatcgggtt ttgccccata tggctggttg aactgtcggc aatagcaaac aggtaaatcg
     2881 ccgccacggg cagcagccac atggtttcaa ttaacatgcc ggtttgtgct tctaccgcaa
     2941 tcttcttgcg caccaggccg tagaaggcaa aactaaatgc cagccccagc gcgataatag
     3001 gtagcgaacc aaaagtccac agctggacca acacgccaca tatcgccaga atcaccgcca
     3061 gccattgcat ccggcggaat cgctcgccga ggaaaatcat ccccagcaca atgttcacca
     3121 gcgggttaat aaagtaacca aggctcgctt ccagcatatg gtgattgttc accgcccaga
     3181 taaacagtag ccagttgcca ccaatcagta cggcagagac tgccagcata aaaattttct
     3241 gtggcgtctg aatcagcgtt tttaaatagg accactggcg gcaaatgctc atcagcacca
     3301 ccataaagaa aaacgaccag atcacgcgat gcgtcaggat ctcatcggcg ggcacgtagt
     3361 aaatcagctt gaagtatgct ggcgcaatac cccaaataaa ataagcggca agagcgagta
     3421 atacgccctg ccgcgtttgt tttgcatcca tcgggaatac tcatttttaa ttggtaacag
     3481 cagtttacct gcttttatgt cttcaaccta ccatataggt ggcggtggca ctggcaatat
     3541 aaagctgttc ttcattgtgt aattcaacgc gggcgacggc gactttattg cctgcacgca
     3601 acaggctact ggtagcagta aaacgctcgc ccctgcctgg gcgcagataa tcaacgcgaa
     3661 gatcaatggt ccccatccgc gatagccgct ggcgtagttc atcttcactg atggtttcgt
     3721 ggcgggttaa ggtacttccc acgcacacca gaccggcggc gacatccagc gccgacgcaa
     3781 tgactccgcc gtgcaaaatg ctttgcgccc agttacccac catcatcggc tgatttttaa
     3841 aggccagctg tgcgaactcc ttttcgtaac gctccagttc catccccaat gcgcggttaa
     3901 atggcatgtg ataaacaaac atctcaccca ctaatttcag ggcttgttca gcggtcagta
     3961 cggcagacat atcatcctta cacttcattg gttaatgaaa tgttgatttt atgcttcttt
     4021 gttgttggtt tctactttag gaagggataa ctaacggcaa tggagttaag tatgtaaaat
     4081 agcccgcaga aaaacattca ccttatcaat aattcgttac ggagaacacg accgatgcgg
     4141 actctgcagg gctggttgtt gccggtgttt atgttgccta tggcagtata tgcacaagag
     4201 gcaacggtga aagaggtgca tgacgcgcca gcggtgcgtg gcagtattat cgccaatatg
     4261 ctgcaggagc atgacaatcc gttcacgctc tatccttatg acaccaacta cctcatctac
     4321 acccaaacca gcgatctgaa taaagaagcg attgccagtt acgactgggc ggaaaatgcg
     4381 cgtaaggatg aagtaaagtt tcagttgagc ctggcatttc cgctgtggcg tgggatttta
     4441 ggcccaaact cggtgttggg tgcgtcttat acgcaaaaat cctggtggca actgtccaat
     4501 agcgaagagt cttcaccgtt tcgtgaaacc aactacgaac cgcaattgtt cctcggtttt
     4561 gccaccgatt accgttttgc aggttggacg ctgcgcgatg tggagatggg gtataaccac
     4621 gactctaacg ggcgttcaga cccgacctcc cgcagctgga accgccttta tactcgcctg
     4681 atggcagaaa acggtaactg gctggtagaa gtgaagccgt ggtatgtggt gggtaatact
     4741 gacgataacc cggatatcac caaatatatg ggttactacc agcttaaaat cggctatcac
     4801 ctcggtgatg cggtgctcag tgcgaaagga cagtacaact ggaacaccgg ctacggcggc
     4861 gcggagttag gcttaagtta cccgatcacc aaacatgtgc gcctttatac tcaggtttac
     4921 agcggctatg gcgaatcgct catcgactat aacttcaacc agacccgtgt cggtgtaggg
     4981 gttatgctaa acgacttgtt ttgatgaacg gttgagtggt tggcaaatct ggaatccagc
     5041 atccaggatt accctctcag agactaaaag cattgcagtt tctcgcgcag gcgctgaaaa
     5101 tagcgcctgt ttttatttca ggcaatcggg gtgaatgtgg cgcaggcgga agtgttgaat
     5161 ctggagtccg gagctaaaca ggttttacaa gaaacctttg gctaccaaca gtttcgcccc
     5221 ggccaggaag aaattatcga cactgtgctt tccggtcgcg attgcctggt tgttatgccc
     5281 actggtggcg gaaaatccct ttgctatcaa attcctgcct tattgctaaa cggccttacc
     5341 gtggttgttt caccgctgat ttcgttgatg aaagatcagg tggatcaact acaggccaac
     5401 ggcgtggcgg cggcgtgcct taactcgacg caaacccgcg aacagcaact tgaagtgatg
     5461 acaggctgcc gcaccgggca aattcgctta ctgtatatcg ccccggaacg cctgatgctg
     5521 gataactttc ttgagcatct ggcgcactgg aatccggtgt tattagccgt cgatgaagcg
     5581 cactgtatct cccaatgggg tcacgatttc cgcccggaat atgccgcgct cggtcagttg
     5641 cgccagcggt tcccgacgct gccgtttatg gcgctgaccg ccacagccga cgacaccacg
     5701 cgccaggata tcgtgcgcct gctggggctg aacgatccgc tgattcaaat cagcagtttt
     5761 gaccgtccga atattcgcta catgctgatg gagaaattca aaccgctcga tcagttgatg
     5821 cgctacgtgc aggaacagcg cggtaagtct ggcattatct actgcaacag ccgggcgaaa
     5881 gtagaagaca ccgctgcgcg cctgcaaagc aggggtatta gcgcggcggc ctatcatgcc
     5941 gggctggaaa ataatgtccg cgccgacgtg caggagaaat tccagcgcga tgatctgcaa
     6001 attgtggtgg cgacggtggc gttcggcatg ggcatcaata aaccaaacgt tcgcttcgtg
     6061 gtccactttg atattccgcg caatatcgaa tcctattatc aggaaaccgg tcgcgccggg
     6121 cgtgatggcc tgcctgcgga agcgatgctg ttttacgatc cggctgatat ggcgtggctg
     6181 cgccgttgtc tggaagagaa gccgcagggg cagttgcagg atatcgagcg ccacaaactc
     6241 aatgcgatgg gcgcgtttgc cgaagcgcaa acttgccgtc gtctggtatt gctgaactat
     6301 tttggcgaag ggcgtcagga gccgtgcggg aactgcgata tctgcctcga tccgccgaaa
     6361 cagtacgacg gttcaaccga tgctcagatt gccctttcca ccattggtcg tgtgaatcag
     6421 cggtttggga tgggttatgt ggtggaagtg attcgtggtg ctaataacca gcgtatccgc
     6481 gactatggtc atgacaaact gaaagtctat ggtatgggcc gtgataaaag ccatgaacat
     6541 tgggtgagcg tgatccgcca gctgattcat ctcggcctgg tgacgcaaaa tattgcccag
     6601 cattctgccc tacaactgac agaggccgcg cgcccggtgc tgcgcggcga atcctctttg
     6661 caacttgccg tgccgcgtat cgtggcgctc aaaccgaaag cgatgcagaa atcgttcggc
     6721 ggcaactatg atcgcaaact gttcgccaaa ttacgcaaac tgcgtaaatc gattgccgat
     6781 gaaagtaatg tcccgccgta cgtggtgttt aacgacgcaa ccttgattga gatggctgaa
     6841 cagatgccga tcaccgccag cgaaatgctc agcgttaacg gcgttgggat gcgcaagctg
     6901 gaacgctttg gtaaaccgtt tatggcgctg attcgtgcgc atgtcgatgg cgacgacgaa
     6961 gagtagtcag cagcataaaa aagtgccagt atgaagactc cgtaaacgtt tcccccgcga
     7021 gtcaaatgta tgttgatgtt atttctcacc gtcgccatgg tgcacattgt ggcgcttatg
     7081 agccccggtc ccgatttctt ttttgtctct cagaccgctg tcagtcgttc ccgtaaagaa
     7141 gcgatgatgg gcgtgctggg cattacctgc ggcgtaatgg tttgggctgg gattgcgttg
     7201 cttggcctgc atttgattat cgaaaaaatg gcctggctgc atacgctgat tatggtaggc
     7261 ggtggcctgt atctctgctg gatgggttac cagatgctac gcggtgcact gaaaaaagag
     7321 gctgtatctg cgcctgcacc acaggttgag ctggcgaaaa gcgggcgcag tttcctgaaa
     7381 ggcttactga ccaatctcgc caatccgaaa gcgattatct attttggttc ggtattctca
     7441 ttgtttgtcg gcgataacgt tggcactacc gagcgctggg gcattttcgc gttgattatt
     7501 gtcgaaacac tggcgtggtt taccgtcgtt gccagtctgt ttgccctgcc gcaaatgcgc
     7561 cgtggttatc aacgtctggc gaagtggatt gatggttttg ccggggcgtt atttgccgga
     7621 tttggcattc atttgattat ttcgcggtga tgccagacgc gtcttcagag taagtcggat
     7681 aaggcgttta cgccgcatcc gacattattt ttcacgcatg cctcgccgat gctaacagcg
     7741 ctcccaccag cataaacaac gagccgaaaa tcttattcag cgccttcatc tgctttggtc
     7801 ctttaatcca tagagcaatc ctttgagcaa gggtggcgta accgatcatc acaataatat
     7861 cgaccacaat agtggtgacg ccgagcacga tatactgcat cagttgcggc tgttgcggca
     7921 tgatgaattg cggaaatagc gccgccagaa acacaatact tttgggattg gtgagattca
     7981 caaaaactgc gcgctggaac aaatgtcgac gcgattgagt agaggccagc gatttaagat
     8041 caattgcgcc agcggcgcgc cactgctgga ttcccagcca aatcaagtaa gccgcgcctg
     8101 cccactttaa cacttcaaac gcaatcactg agcgggaaaa tagcgtcccc aaccccacgc
     8161 caaccagcac aatatgaatc gccagtccgg tctgaagccc agcaatagac gccaccgcgc
     8221 cgcgataacc gtggttgagc gaggtggtca tagtgttgat tgcaccagag cctggcgaca
     8281 ggcttaaaat gatcgatgtc agcaggtagg caaaccacca ttctaaggtc atgatgaact
     8341 cccggtgtgt ctatttttgt gccacaatac gctactgtcg cagcgttgtg tcaggcacgc
     8401 ttaaaaaaac gattttacgt ggtttaagag gcagattacc cgatgtttca gcagcaaaaa
     8461 gactgggaaa caagagaaaa cgcgtttgct gcttttacca tgggaccgct gactgatttc
     8521 tggcgtcagc gtgatgaagc agagtttact ggtgtggatg acattccggt gcgctttgtc
     8581 cgttttcgcg cacagcacca tgaccgggtg gtagtcatct gcccggggcg tattgagagc
     8641 tacgtaaaat atgcggaact ggcctacgac ctgttccatt tggggtttga tgtcttaatc
     8701 atcgaccatc gcgggcaggg acgttccggt cgcctgttag ccgatccgca tctcgggcat
     8761 gttaatcgct ttaatgatta tgttgatgat ctggcggcat tctggcagca ggaggttcag
     8821 cccggtccgt ggcgtaaacg ctatatactg gcacattcga tgggcggtgc gatctccaca
     8881 ttatttctgc aacgccatcc aggtgtatgt gacgccattg cgctaactgc gccaatgttt
     8941 gggatcgtga ttcgtatgcc gtcatttatg gcacggcaga tcctcaactg ggccgaagcg
     9001 catccacgtt tccgtgatgg ctatgcaata ggcaccgggc gctggcgcgc gttgccgttt
     9061 gctatcaacg tactgaccca cagcagacag cgatatcgac gtaacttacg cttctatgct
     9121 gatgacccaa cgattcgcgt cggtgggccg acctaccatt gggtacgcga aagtattctg
     9181 gctggcgaac aggtgttagc cggtgcgggt gatgacgcca cgcaaacgct tctcttacaa
     9241 gctgaagagg aacgcgtggt ggataaccgc atgcacgacc gtttttgtga actccgcacc
     9301 gccgcgggcc atcctgtcga aggaggacgg ccgttggtaa taaaaggtgc ttaccatgag
     9361 atcctttttg aaaaggacgc aatgcgctca gtcgcgctcc acgccatcgt tgattttttc
     9421 aacaggcata actcacccag cggaaaccgc tctacagagg tttaaatttc ttatgtacca
     9481 ggttgttgcg tctgatttag atggcacgtt actttctccc gaccatacgt tatcccctta
     9541 cgccaaagaa actctgaagc tgctcaccgc gcgcggcatc aactttgtgt ttgcgaccgg
     9601 tcgtcaccac gttgatgtgg ggcaaattcg cgataatctg gagattaagt cttacatgat
     9661 tacctccaat ggtgcgcgcg ttcacgatct ggatggcaat ctgatttttg ctcataacct
     9721 ggatcgcgac attgccagcg atctgtttgg cgtagtcaac gacaatccgg acatcattac
     9781 taacgtttat cgcgacgacg aatggtttat gaatcgccat cgcccggaag agatgcgctt
     9841 ttttaaagaa gcggtgttca aatatgcgct gtatgagccg ggattactgg agccggaagg
     9901 catcagcaaa gtgtttttca cctgcgattc ccatgaacaa ctgctgccgc tggagcaggc
     9961 gattaacgct cgctggggcg atcgcgtcaa cgtcagtttc tctaccttaa cctgtctgga
    10021 agtgatggcg ggcggcgtgt cgaaaggcca tgcgctggaa gcagtggcga agaaactggg
    10081 ctacagcctg aaggattgta ttgcgtttgg tgacgggatg aacgacgccg aaatgctgtc
    10141 gatggcgggg aaaggctgca ttatgggcag tgcgcaccag cgtctgaaag atctgcatcc
    10201 ggagctggaa gttattggta ctaatgccga cgacgcggtg ccgcattatc tgcgtaaact
    10261 ctatttatcg taatcgttct ttatttggtc agttgtcaac ctgatacttc gctacaatgg
    10321 atactcgtta atcaaagagt tttccattgt ggcgttactt atcatcacca cgattctgtg
    10381 ggccttctcc tttagctttt atggcgagta ccttgcgggg cacgtcgata gctattttgc
    10441 ggtgctggtg cgcgttggct tggcggcact cgtttttctg ccgtttctgc gtacccgtag
    10501 caatagcctg aaaacggtcg gcctgtatat gctggtgggc gcgatgcagc tcggcgtgat
    10561 gtatatgctg agtttccgcg cttatctcta cctgacggtc tccgaattgt tgctgttcac
    10621 cgtactgacg ccgctctaca tcacgctgat ttatgacatc atgagtaagc gccgtctgcg
    10681 ctggggctat gcttttagcg ccttgctggc ggtgattggt gccgggatta ttcgctatga
    10741 tcaggtcacc gaccatttct ggactggctt gttgctggtg caactctcca atatcacttt
    10801 tgccatcggc atggtgggtt acaaacgcct gatggaaact cgcccgatgc cgcagtataa
    10861 cgcctttgcg tggttctatc ttggcgcgtt cctggtggcg gtaattgcat ggttcttgct
    10921 gggaaatgcg cagaaaatgc cgcaaaccac gttgcaatgg ggcattctgg tgtttcttgg
    10981 cgtggtggct tccgggattg gctactttat gtggaactac ggcgcgacgc aggtggacgc
    11041 cggaacgctg ggcattatga ataatatgca cgttccggca gggctgctgg taaacctggc
    11101 tatctggcac caacagccgc actggccaac gtttattaca ggcgcgctgg tgatcctggc
    11161 ctcactgtgg gtgcatcgta agtgggtcgc tccgcgctct tcacaaacgg cagatgatcg
    11221 caggcgtgat tgcgcgctga gcgaataaac gcttccgtaa ctggctgacg ctgctcgcca
    11281 tcgcgcacgg cggcgtacag tcggctccac aagccttcgc ccagggtttt ggtcaccacc
    11341 agaccctggc gctcaaaact ctctactacc caatgcggta gcgcggcaat acccatccgc
    11401 gcggcaacca tttgaatcaa caataaggtg ttatcgacgc ttttcagtga cgggctgacg
    11461 cctgccggct gaagaaaatg ccgccagaca tccagtcgac tacgctgcac cggataaatt
    11521 aatagcgtct cgctggcgag atcttccggt gtaatacgcg tttttgccgc cagtggatgg
    11581 tcaggtgcta acaccaggcg tacttcataa tcgaacatcg gcgaataatg caggccactg
    11641 cgcggcagga tatcggaggt catcaccaga tccagctcgc cctgttgcaa cgcgggctgc
    11701 gggtcaaacg tcacgcccga tttaaaatcc atctctacct gcggccagtt cttatggaaa
    11761 tttcctaaag cgggtgtcag ccactgaata cagctatggc actcaatggc aatgcgcaga
    11821 cgcgtctgct gcggttcatt gcaggcttgc agtgcctggc tgatttgcgg cagcacctgg
    11881 tttgccagtt gcaacaggat ttctccctgc ggtgtaaagc gtagcggctg gctcttacgc
    11941 acaaatagcc ggaagccaag gcgttgttcc agatcgctaa actggtgaga cagggcggat
    12001 tgcgtctgat gcaacgttgc cgcagcggct gcgagcgagc cgcagttccg caacgcttgt
    12061 agcgttttca ggtgttttac ttcgatcatg aaagtccttc acttcggcat gaataatttg
    12121 cgcttgagga atatacagta accgccaatt atggatgtgt aaacatctgg acggctaaaa
    12181 tcattcgtct tttaaattta tggtgcgttg gctgcgtttc tccaccccgg tcacttactt
    12241 cagtaagctc ccggcgatga ataaacttgc cgccttccct aaattcaaaa tccataggat
    12301 ttacatataa ttagaggaag aaaaatgaca atattgaatc acaccctcgg tttccctcgc
    12361 gttggcctgc gtcgcgagct gaaaaaagcg caagaaagtt attgggcggg gaactccacg
    12421 cgtgaagaac tgctgacagt agggcgtgaa ctgcgtgctc gtcactggga tcaacaaaag
    12481 caagcgggta tcgacctgct gccggtgggc gattttgcct ggtacgatca tgtactgacc
    12541 accagtttgc tgctgggtaa tgttccgcca cgtcatcaga acaaagacgg ttcggtagat
    12601 atcgacaccc tgttccgtat tggtcgtgga cgtgcgccga ctggcgaacc tgcggcggca
    12661 gcggaaatga ccaaatggtt taacaccaac tatcactaca tggtgccgga gttcgttaaa
    12721 ggccaacagt tcaaactgac ttggacgcag ctgctggagg aagtggacga ggcgctggcg
    12781 ctgagccaca acgttaagcc tgtgctgctg gggccggtta cttacctgtg gctggggaaa
    12841 gtgaaaggtg aacagtttga ccgccttagc ctgctgaacg acattctgcc ggtttatcag
    12901 caagtgctgg cagaactggc gaaacgcggc atcgagtggg tacagattga tgaacccgcg
    12961 ctggtactgg aactaccaca ggcgtggctg gacgcataca aacccgctta cgacgcgctc
    13021 cagggacagg tgaaactgct gctgaccacc tattttgaag gcgtaacgcc aaatctcgac
    13081 acgattactg cgctgcctgt tcaggggctg catgtggacc ttgtacatgg taaagataac
    13141 gttgttgaac tgcacaagcg cctgccttct gactggctgc tgtctgcggg tctgatcaat
    13201 ggtcgtaacg tctggcgcgc cgatcttact gagaaatatg cgcaaattaa ggacattgtc
    13261 ggcaaacgtg atttgtgggt ggcatcttcc tgctcgttgc tgcacagccc catcgacctg
    13321 agcgtggaaa cgcgtcttga tgcagaagtg aaaagctggt ttgccttcgc cctacaaaaa
    13381 tgccatgaac tggcactgct gcgcgatgcg ctgaacagtg gtgacacggc agctctggca
    13441 gagtggagcg ccccaattca ggcgcgtcgt cactctactc gtgtacataa tccggcggta
    13501 gaaaagcgtc tggcggcgat caccgcccag gacagccagc gtgcgaatgt ctatgaagtg
    13561 cgtgctgaag cccagcgtgc gcgttttaaa ctgccagcgt ggccgaccac cacgattggt
    13621 tccttcccgc aaaccacgga aattcgtacc ctgcgtctgg atttcaaaaa gggcaatctc
    13681 gacgccaaca actaccgcac gggcattgcg gaacatatca agcaggccat tgttgagcag
    13741 gaacgtttgg gactggatgt gctggtacat ggcgaggccg agcgtaatga catggtggaa
    13801 tactttggcg agcacctcga cggatttgtc tttacgcaaa acggttgggt acagagctac
    13861 ggttcccgct gcgtgaagcc accgattgtc attggtgaca ttagccgccc ggcaccgatt
    13921 accgtggagt gggcgaagta tgcgcaatcg ctgaccgaca aaccggtgaa agggatgctg
    13981 acggggccgg tgaccatact ctgctggtcg ttcccgcgtg aagatgtcag ccgtgaaacc
    14041 atcgccaaac agattgcgct ggcgctgcgt gatgaagtgg ccgatctgga agccgctgga
    14101 attggcatca tccagattga cgaaccggcg ctgcgcgaag gtttaccgct gcgtcgtagc
    14161 gactgggatg cgtatctcca gtggggcgta gaggccttcc gtatcaacgc cgccgtggcg
    14221 aaagatgaca cacaaatcca cactcacatg tgttattgcg agttcaacga catcatggat
    14281 tcgattgcgg cgctggacgc agacgtcatc accatcgaaa cctcgcgttc cgacatggag
    14341 ttgctggagt cgtttgaaga gtttgattat ccaaatgaaa tcggtcctgg cgtctatgac
    14401 attcactcgc caaacgtacc gagcgtggaa tggattgaag ccttgctgaa gaaagcggca
    14461 aaacgcattc cggcagagcg cctgtgggtc aacccggact gtggcctgaa aacgcgcggc
    14521 tggccagaaa cccgcgcggc actggcgaac atggtgcagg cggcgcagaa tttgcgtcgg
    14581 gggtaaatcc aaaccgggtg gtaataccac ccggtctttt ctcattacag cgacttcttc
    14641 ccaccatact gcttaaacca ttccagcata cgctgccagc catctttcgc agattcggca
    14701 tgatagctcg ggcgataatc agcgttgaat gcatgcccgg catccgggta cacgataatc
    14761 tctgctttcg cattagcagc ccgcagcgcc tggcgcatcg tttcaacgct ctcctgcgga
    14821 atgctgttat cctgaccacc atataagccg agaaccggcg cgttaagatc ggttgcgata
    14881 tcaacaggtt gtttcggtga attcagcgac ttgtcgcccg tcagtttgcc gtaccacgcc
    14941 actgcggctt ttagctgtgg attatgcgcg gcatacagcc aggtgatacg tccaccccag
    15001 cagaatccgg tgatcattaa acgatgaaca tcgccgccgt tgcgggaagc ccaactggcg
    15061 acatgatcga gatcggccag cacctgcgag tcaggcactt ttgctaccag accgctaagc
    15121 aacgtgggga tatcggcaaa atcattcgga tcgccttcgc ggaagtaaag ttcaggtgcg
    15181 atagccagat acccctccag cgccagacgg cgacaaatgt cgcggatatg ttcatgcacg
    15241 ccaaaaattt cctgcactac aatgaccact ggcagtgggc catcgctttg ctttggtctg
    15301 gcatggtaag caggcatgtt atccccttgt gaagggatag aggtgaagcc cgccacaatc
    15361 gcgtcgtccg gggtctgaac gatggtcgaa gcgagaggcg atgcagcagg tgcaaatcca
    15421 gattgttgtg ttgttgccat ggtattctcc gtacccttat aaaaatgttg cgcaatgtta
    15481 actatagtca gcatgcaaca aatcacattg cctgaatcgg ctcatctttt atgcagtcct
    15541 gcagaatgaa gagtgattta tgtgatatga atcacttttg gtgggtaaat ttatgcaacg
    15601 catttgcgtc atggtgatga gtatcacgaa aaaatgttaa acccttcggt aaagtgtctt
    15661 tttgcttctt ctgactaaac cgattcacag aggagttgta tatgtccaag tctgatgttt
    15721 ttcatctcgg cctcactaaa aacgatttac aaggggctac gcttgccatc gtccctggcg
    15781 acccggatcg tgtggaaaag atcgccgcgc tgatggataa gccggttaag ctggcatctc
    15841 accgcgaatt cactacctgg cgtgcagagc tggatggtaa acctgttatc gtctgctcta
    15901 ccggtatcgg cggcccgtct acctctattg ctgttgaaga gctggcacag ctgggcattc
    15961 gcaccttcct gcgtatcggt acaacgggcg ctattcagcc gcatattaat gtgggtgatg
    16021 tcctggttac cacggcgtct gtccgtctgg atggcgcgag cctgcacttc gcaccgctgg
    16081 aattcccggc tgtcgctgat ttcgaatgta cgactgcgct ggttgaagct gcgaaatcta
    16141 ttggcgcgac aactcacgtt ggcgtgacag tttcttctga taccttctac ccaggtcagg
    16201 aacgttacga tacttactct ggtcgcgtag ttcgtcactt taaaggttct atggaagagt
    16261 ggcaggcgat gggcgtaatg aactatgaaa tggaatctgc aactctgctg accatgtgtg
    16321 caagtcaggg cctgcgtgcc ggtatggtag cgggtgttat cgttaaccgc acccagcaag
    16381 agatcccgaa tgctgagacg atgaaacaaa ccgaaagcca tgcggtgaaa atcgtggtgg
    16441 aagcggcgcg tcgtctgctg taattctctt ctcctgtctg aaggccgacg cgttcggcct
    16501 tttgtatttt tgcgtagcgc ctcgcaggaa atgcctttcc aactggacgt ttgtacagca
    16561 caattctatt ttgtgcgggt aagttgttgc gtcaggaggc gttgtggatt tctcaatcat
    16621 ggtttacgca gttattgcgt tggtgggtgt ggcaattggc tggctgtttg ccagttatca
    16681 acatgcgcag caaaaagccg agcaattagc tgaacgtgaa gagatggtcg cggagttaag
    16741 cgcggcaaaa caacaaatta cccaaagcga gcactggcgt gcagagtgtg aattgctcaa
    16801 taacgaagtg cgcagcctgc aaagtattaa cacctctctg gaggccgatc tgcgtgaagt
    16861 aaccacgcgg atggaagccg cacagcaaca tgctgacgat aaaattcgcc agatgattaa
    16921 cagcgagcag cgcctcagtg agcagtttga aaacctcgcc aaccgtattt ttgagcacag
    16981 caatcgccgg gttgatgagc aaaaccgtca gagtctgaac agcctgttgt cgccgctacg
    17041 tgaacaactg gacggtttcc gccgtcaggt tcaggacagc ttcggtaaag aagcacaaga
    17101 acgccatacc ctgacccacg aaattcgcaa tctccagcaa ctcaacgcgc aaatggccca
    17161 ggaagcgatc aacctgacgc gcgcgctgaa aggcgacaat aaaacccagg gcaactgggg
    17221 cgaggtagta ttgacgcggg tgctggaggc ttccggtctg cgtgaagggt atgaatatga
    17281 aacccaggtc agcatcgaaa atgacgcccg ctcgcggatg cagccggatg tcatcgtgcg
    17341 gctgccgcag ggaaaagatg tggtgatcga cgccaaaatg acgctggtcg cctatgaacg
    17401 ctattttaac gccgaagacg actacacccg cgaaagcgcg ctacaggaac atatcgcgtc
    17461 ggtgcgtaac catatccgtt tgctgggacg caaagattat caacagctgc cggggctgcg
    17521 aactctggat tacgtgctga tgtttattcc cgttgaaccc gcttttttac tggcgcttga
    17581 ccgccagccg gagctgatca ccgaagcgtt gaaaaacaat atcatgctgg ttagcccgac
    17641 tacgctgctg gtggcgttgc gtactattgc caacctgtgg cgttatgagc atcaaagccg
    17701 taacgcccag caaatcgccg atcgtgccag caagctgtac gacaagatgc gtttgttcat
    17761 cgatgacatg tccgcgattg gtcaaagtct cgacaaagcg caggataact accgccaggc
    17821 aatgaaaaaa ctctcttcag gtcgcggaaa tgtgctggcg caggcagaag cgtttcgcgg
    17881 tttaggagta gaaattaaac gcgagattaa tccggatttg gctgaacagg cggtgagcca
    17941 ggatgaagag tatcgacttc ggtcggttcc ggagcagccg aatgatgaag cttatcaacg
    18001 cgatgatgaa tataatcagc agtcgcgcta gcccattagg ggtagttaag ccgggtagaa
    18061 atctagggca tcgacgccca atctgttaca cttctggaac aattttttga tgagcaggca
    18121 ttgagatggt ggataagtca caagaaacga cgcactttgg ttttcagacc gtcgcgaagg
    18181 aacaaaaagc ggatatggtc gcccacgttt tccattccgt ggcatcaaaa tacgatgtca
    18241 tgaatgattt gatgtcattt ggtattcatc gtttgtggaa gcgattcacg attgattgca
    18301 gcggcgtacg ccgtgggcag accgtgctgg atctggctgg tggcaccggc gacctgacag
    18361 cgaaattctc ccgcctggtc ggagaaactg gcaaagtggt ccttgctgat atcaatgaat
    18421 ccatgctcaa aatgggacgc gagaagctgc gtaatatcgg tgtgattggc aacgttgagt
    18481 atgttcaggc gaacgctgag gcgctgccgt tcccggataa cacctttgat tgcatcacca
    18541 tttcgtttgg tctgcgtaac gtcaccgaca aagataaagc actgcgttca atgtatcgcg
    18601 tgctgaaacc cggcggccgc ctgctggtgc ttgagttctc gaagccaatt atcgagccgc
    18661 tgagcaaagc ctatgatgca tactccttcc atgtgctgcc gcgtattggc tcactggtcg
    18721 cgaacgacgc cgacagctac cgttatctgg cagaatccat ccgtatgcat cccgatcagg
    18781 ataccctgaa agccatgatg caggatgccg gattcgaaag tgtcgactac tacaatctga
    18841 cggcaggggt tgtggcgctg catcgtggtt ataagttctg acaggagacc ggaaatgcct
    18901 tttaaacctt tagtgacggc aggaattgaa agtctgctca acaccttcct gtatcgctca
    18961 cccgcgctga aaacagcccg ctcgcgtctg ctgggtaaag tattgcgcgt ggaggtaaaa
    19021 ggcttttcga cgtcattgat tctggtgttc agcgaacgcc aggttgatgt actgggcgaa
    19081 tgggcaggcg atgctgactg caccgttatc gcctacgcca gtgtgttgcc gaaacttcgc
    19141 gatcgccagc agcttaccgc actgattcgc agtggtgagc tggaagtgca gggcgatatt
    19201 caggtggtgc aaaacttcgt tgcgctggca gatctggcag agttcgaccc tgcggaactg
    19261 ctggcccctt ataccggtga tatcgccgct gaaggaatca gcaaagccat gcgcggaggc
    19321 gcaaagttcc tgcatcacgg cattaagcgc cagcaacgtt atgtggcgga agccattact
    19381 gaagagtggc gtatggcacc cggtccgctt gaagtggcct ggtttgcgga agagacggct
    19441 gccgtcgagc gtgctgttga tgccctgacc aaacggctgg aaaaactgga ggctaaatga
    19501 cgccaggtga agtacggcgc ctatatttca tcattcgcac ttttttaagc tacggacttg
    19561 atgaactgat ccccaaaatg cgtatcaccc tgccgctacg gctatggcga tactcattat
    19621 tctggatgcc aaatcggcat aaagacaaac ttttaggtga gcgactacga ctggccctgc
    19681 aagaactggg gccggtttgg atcaagttcg ggcaaatgtt atcaacccgc cgcgatcttt
    19741 ttccaccgca tattgccgat cagctggcgt tattgcagga caaagttgct ccgtttgatg
    19801 gcaagctggc gaagcagcag attgaagctg caatgggcgg cttgccggta gaagcgtggt
    19861 ttgacgattt tgaaatcaag ccgctggctt ctgcttctat cgcccaggtt cataccgcgc
    19921 gattgaaatc gaatggtaaa gaggtggtga ttaaagtcat ccgcccggat attttgccgg
    19981 tcattaaagc ggatctgaaa cttatctacc gtctggctcg ctgggtgccg cgtttgctgc
    20041 cggatggtcg ccgtctgcgc ccaaccgaag tggtgcgcga gtacgaaaag accttgattg
    20101 atgaactgaa tttgctgcgg gaatctgcca acgccattca gcttcggcgc aattttgaag
    20161 acagcccgat gctctacatc ccggaagttt accctgacta ttgtagtgaa gggatgatgg
    20221 tgatggagcg catttacggc attccggtgt ctgatgttgc ggcgctggag aaaaacggca
    20281 ctaacatgaa attgctggcg gaacgcggcg tgcaggtgtt cttcactcag gtctttcgcg
    20341 acagcttttt ccatgccgat atgcaccctg gcaacatctt cgtaagctat gaacacccgg
    20401 aaaacccgaa atatatcggc attgattgcg ggattgttgg ctcgctaaac aaagaagata
    20461 aacgctatct ggcagaaaac tttatcgcct tctttaatcg cgactatcgc aaagtggcag
    20521 agctacacgt cgattctggc tgggtgccac cagataccaa cgttgaagag ttcgaatttg
    20581 ccattcgtac ggtctgtgaa cctatctttg agaaaccgct ggccgaaatt tcgtttggac
    20641 atgtactgtt aaatctgttt aatacggcgc gtcgcttcaa tatggaagtg cagccgcaac
    20701 tggtgttact ccagaaaacc ctgctctatg tcgaaggggt aggacgccag ctttatccgc
    20761 agctcgattt atggaaaaca gcgaagcctt tcctggagtc gtggattaaa gatcaggtcg
    20821 gtattcctgc cctggtgaga gcatttaaag aaaaagcgcc gttctgggtc gaaaaaatgc
    20881 cagaactgcc tgaactggtt tacgacagtt tgcgccaggg caagtattta cagcacagtg
    20941 ttgataagat tgcccgcgag cttcagtcaa atcatgtacg tcagggacaa tcgcgttatt
    21001 ttctcggaat tggcgctacg ttagtattaa gtggcacatt cttgttggtc agccgacctg
    21061 agtgggggct gatgcccggc tggttaatgg caggtggtct gatcgcctgg tttgtcggtt
    21121 ggcgcaaaac acgctgattt tttcatcgct caaggcgggc cgtgtaacgt ataatgcggc
    21181 tttgtttaat catcatctac cacagaggaa catgtatggg tggtatcagt atttggcagt
    21241 tattgattat tgccgtcatc gttgtactgc tttttggcac caaaaagctc ggctccatcg
    21301 gttccgatct tggtgcgtcg atcaaaggct ttaaaaaagc aatgagcgat gatgaaccaa
    21361 agcaggataa aaccagtcag gatgctgatt ttactgcgaa aactatcgcc gataagcagg
    21421 cggatacgaa tcaggaacag gctaaaacag aagacgcgaa gcgccacgat aaagagcagg
    21481 tgtaatccgt gtttgatatc ggttttagcg aactgctatt ggtgttcatc atcggcctcg
    21541 tcgttctggg gccgcaacga ctgcctgtgg cggtaaaaac ggtagcgggc tggattcgcg
    21601 cgttgcgttc actggcgaca acggtgcaga acgaactgac ccaggagtta aaactccagg
    21661 agtttcagga cagtctgaaa aaggttgaaa aggcgagcct cactaacctg acgcccgaac
    21721 tgaaagcgtc gatggatgaa ctacgccagg ccgcggagtc gatgaagcgt tcctacgttg
    21781 caaacgatcc tgaaaaggcg agcgatgaag cgcacaccat ccataacccg gtggtgaaag
    21841 ataatgaagc tgcgcatgag ggcgtaacgc ctgccgctgc acaaacgcag gccagttcgc
    21901 cggaacagaa gccagaaacc acgccagagc cggtggtaaa acctgctgcg gacgctgaac
    21961 cgaaaaccgc tgcaccttcc ccttcgtcga gtgataaacc gtaaacatgt ctgtagaaga
    22021 tactcaaccg cttatcacgc atctgattga gctgcgtaag cgtctgctga actgcattat
    22081 cgcggtgatc gtgatattcc tgtgtctggt ctatttcgcc aatgacatct atcacttggt
    22141 atccgcgcca ttgatcaagc agttgccgca aggttcaacg atgatcgcca ccgacgtggc
    22201 ctcgccgttc tttacgccga tcaagctgac ctttatggtg tcgctgattc tgtcagcgcc
    22261 ggtgattctc tatcaggtgt gggcatttat cgccccagcg ctgtataagc atgaacgtcg
    22321 cctggtggtg ccgctgctgg tttccagctc tctgctgttt tatatcggca tggcattcgc
    22381 ctactttgtg gtctttccgc tggcatttgg cttccttgcc aataccgcgc cggaaggggt
    22441 gcaggtatcc accgacatcg ccagctattt aagcttcgtt atggcgctgt ttatggcgtt
    22501 tggtgtctcc tttgaagtgc cggtggcaat tgtgctgctg tgctggatgg ggattacctc
    22561 gtcagaagac ttacgcaaaa aacgcccgta tgtgctggtt ggtgcattcg ttgtcgggat
    22621 gttgctgacg ccgccggatg tcttctcgca aacgctattg gcgatcccga tgtactgtct
    22681 gtttgaaatc ggtgtcttct tctcacgctt ttacgttggt aaagggcgaa atcgggaaga
    22741 ggaaaacgac gctgaagcag aaagcgaaaa aactgaagaa taaattcaac cgcccgtcag
    22801 ggcggttgtc atatggagta caggatgttt gatatcggcg ttaatttgac cagttcgcaa
    22861 tttgcgaaag accgtgatga tgttgtagcg cgcgcttttg acgcgggagt taatgggcta
    22921 ctcatcaccg gcactaacct gcgtgaaagc cagcaggcgc aaaagctggc gcgtcagtat
    22981 tcgtcctgtt ggtcaacggc gggcgtacat cctcacgaca gcagccagtg gcaagctgcg
    23041 actgaagaag cgattattga gctggccgcg cagccagaag tggtggcgat tggtgaatgt
    23101 ggtctcgact ttaaccgtaa cttttcgacg ccggaagagc aggaacgcgc ttttgttgct
    23161 cagctacgca ttgccgcaga attaaacatg ccggtattta tgcactgtcg cgatgcccac
    23221 gagcggttta tgacattgct ggagccgtgg ctggataaac tgcctggtgc ggttcttcat
    23281 tgctttaccg gcacacgcga agagatgcag gcgtgcgtgg cgcgtggaat ttatatcggc
    23341 attaccggtt gggtttgcga tgaacgacgc gggctggagc tgcgggaatt gttgccgttg
    23401 attccggcgg agaaattgct gatcgaaaca gatgcgccgt atctgctccc gcgcgatctc
    23461 acgccaaagc catcatcccg gcgcaacgag ccagcccatc tgccccatat tttgcaacgt
    23521 attgcgcact ggcgtggaga agatgccgca tggctggctg ccaccacgga tgccaatgtc
    23581 aaaacactgt ttgggattgc gttttagagt ttgcggaact cggtgttctt cacactgtgc
    23641 ttaatctctt tattaataag attaagcaat agcatggagc gagcctcacc atcgggttcg
    23701 gtgaaaatgg cctgaaagcc ttcgaacgcg ccttcggtaa taatcacctt atcacccgga
    23761 taaggggggg ccggatcgac aatgtctttc ggtttatata ccgatagctg atgaattacc
    23821 gccgatggga ctatcgctgg cgacgcgcca aagcgcacaa agtggctgac gccacgcgta
    23881 gcgctgatgg tcgtggtatg aatcacttct ggatcaaatt ccacaaacag gtagttaggg
    23941 aataacggtt cactgactgc ggtacgttta ccccgtacga ttttttccag ggtgatcatc
    24001 ggagcgaggc aattaaccgc ctgtctctca aggtgttcct gggcacgttg aagttgcccg
    24061 cgcttgcagt acagtaaata ccaggattgc ataatgactc ttatccgttt aatcggggcg
    24121 caaggatagc aaaagcttta cgctaagtta attatattcc ccggtttgcg ttataccgtc
    24181 agagttcacg ctaatttaac aaatttacag cattgcaaag atgaacgccg tataatgggc
    24241 gcagattaag aggctacaat ggacgccatg aaatataacg atttacgcga cttcctgacg
    24301 ctgcttgaac agcagggtga gctaaaacgt atcacgctcc cggtggatcc gcatctggaa
    24361 atcactgaaa ttgctgaccg cactctgcgt gctggtgggc ctgcgctgtt gttcgaaaac
    24421 cctaaaggct actcaatgcc ggtgctgtgc aacctgttcg gtacgccaaa gcgcgtggcg
    24481 atgggcatgg ggcaggaaga tgtttcggcg ctgcgtgaag ttggtaaatt attggcgttt
    24541 ctgaaagagc cggagccgcc aaaaggtttc cgcgacctgt ttgataaact gccgcagttt
    24601 aagcaggtgt taaacatgcc gacaaagcga ctgcgtggtg cgccctgcca acaaaaaatc
    24661 gtctctggcg atgacgtcga tctcaatcgc attcccatta tgacctgctg gccggaagat
    24721 gccgcgccgc tgattacctg ggggctgacc gtaacgcgcg gcccgcataa agagcggcag
    24781 aatctgggca tttatcgcca gcagctgatt ggtaaaaaca aactgattat gcgctggctg
    24841 tcgcatcgcg gcggcgcgct ggattatcag gagtggtgtg cggcgcatcc gggcgaacgt
    24901 ttcccggttt ctgtggcgct gggtgccgat cctgccacga ttctcggtgc agtcaccccc
    24961 gttccggata cgctttcaga gtatgcgttt gccggattgc tacgcggcac caaaaccgaa
    25021 gtagtaaagt gtatttccaa tgacctcgaa gtgccagcca gtgccgaaat cgtcctggaa
    25081 gggtatatcg atcctggtga gatggcgccg gaagggccgt atggtgacca cacaggttac
    25141 tataatgaag tcgataattt cccggtgttt accgtgacgc atattaccca gcgtgaagat
    25201 gcgatttacc attccaccta taccgggcgt ccgccagatg agcccgcggt actgggcgtg
    25261 gcgttgaacg aagtgtttgt accgattctg caaaaacagt tcccggaaat tgtcgatttt
    25321 tacctgccgc cggaaggctg ttcttatcgt ctggcggtag tgacgatcaa aaaacagtac
    25381 gccggacacg cgaagcgcgt catgatgggc gtctggtcgt tcttacgcca gtttatgtac
    25441 actaaatttg tgatcgtttg cgatgatgac gtcaacgcac gcgactggaa cgatgtgatt
    25501 tgggcgatta ccacccgtat ggacccggcg cgggatactg ttctggtaga aaatacgcct
    25561 attgattatc tggattttgc ctcgcctgtc tctgggctgg gttcaaaaat ggggctggat
    25621 gccacgaata aatggccggg ggaaacccag cgtgaatggg gacgtcccat caaaaaagat
    25681 ccagatgttg tcgcgcatat tgacgccatc tgggatgaac tggctatttt taacaacggt
    25741 aaaagcgcct gatgcgcgtt tgttttgccc tatttatcga tccgacagag aaagcgcatg
    25801 acaaccttaa gctgtaaagt gacctcggta gcagctatca cggataccgt atatcgtgtc
    25861 cgcatcgtgc cagacgcggc cttttctttt cgtgctggtc agtatttgat ggtagtgatg
    25921 gatgagcgcg acaaacgtcc gttctcaatg gcctcgacgc cggatgaaaa agggtttatc
    25981 gagctgcata ttggcgcttc tgaaatcaac ctttacgcga aagcagtcat ggaccgcatc
    26041 ctcaaagatc atcaaatcgt ggtcgacctt ccccacggag aagcgtggct gcgcgatgat
    26101 gaagagcgtc cgatgatttt gattgcgggc ggcaccgggt tctcttatgc ccgctcgatt
    26161 ttgctgacag cgttggcgcg taacccaaac cgtgatatca ccatttactg gggcgggcgt
    26221 gaagagcagc atctgtatga tctctgcgag cttgaggcgc tttcgttaaa gcaccttggt
    26281 ctgcaagtgg tgccggtggt tgaacaaccg gaagcaggct ggcgtgggcg tactggtacc
    26341 gtgttaacgg cggtattgca ggatcatggt acgctggcag agcatgatat ctatattgcc
    26401 ggacgttttg agatggcgaa aattgcccgt gacctgtttt gcagtgagcg taatgcgcgg
    26461 gaagatcgcc tgtttggcga tgcgtttgca tttatctgag atataaaaaa acccgcccct
    26521 gacaggcggg aagaacggca actaaaactt tattcagtgg catttagatc tatgtcgcat
    26581 ccggcaattg gtgcacgatg cctgatgcga cgctggcgcg tcttatcagg cctacattgg
    26641 tgccggatcg gtagaccgga taaggcgttc acgccgcatc cggcaagtgg ttaaacccgc
    26701 tcaaacaccg tcgcaatacc ctgacccaga ccgatacaca tcgtcgccag accaaactga
    26761 acgtctttgc gttccatcag attcagcagc gtggtgctga tacgcgcacc ggaacaaccc
    26821 agcggatgac ccagcgcgat cgcaccacca ttgaggttga tcttctcgtc aatctgctcc
    26881 attagcccga gatctttaat gcacggcagg atctgcgcgg caaaggcttc gttcatctca
    26941 aacacgccga tatcgctggc agaaagcccc gcttttttca gcgccagctt cgaggccgga
    27001 accgggccgt aacccataat cgatgggtca caaccaacga ccgccatcga acgcacacga
    27061 gcgcgcggct taagacctaa ttcatgggcg cggctttcac tcatcaccag catggcagct
    27121 gcgccatcgg aaagtgcaga agatgtgccc gccgttaccg taccgtttac tggatcaaac
    27181 gccggacgca gcgtggcgag ggcttccacg gtggtttccg ggcgaatcac ttcgtcgtaa
    27241 ttaaactgct tcaggacgcc gtcggcatcg tgaccaccag ttgggatgat ttcattttta
    27301 aatgcggccg actgcgtggc ggcccaggcg cgggcgtgtg accgcgcggc aaaggcatcc
    27361 tgcatttcac ggctgatacc gtgcatacgc gccagcattt ctgccgttaa gcccatcatg
    27421 cccgccgctt tggcgacatt gcggctcagg ccggggtgaa aatcgacgcc gtgactcatc
    27481 ggcacatggc ctatatgctc cacgccgcca accagacatg cctgcgtatc gccagtcatg
    27541 atcattcgtg ctgcgtcatg cagtgcctgc atggatgaac cacacaagcg attgacggta
    27601 accgccggga cagagtgcgg cacttctgcc agcagcgccg cgttacgggc gatgttaaaa
    27661 ccctgctcca gcgtctgttg cacacaaccc cagtaaatat cgtcgagggc cgccgcttcc
    27721 agcgccgggt tacgcgccag caggctacgc attaaatgag cggagagatc ttccgcacgc
    27781 acgttacgaa aagcaccgcc cttcgaacgg cccatcgggg tgcgaattgc atcgacaatg
    27841 acaacctgtt ccattgtgac tccttaagcc gttttcaggt cgccaaccgg acgggctggc
    27901 tcaaccggag gatagtacgg ttcgttatga cgcgctttat tacgcagacc ttccggcact
    27961 tcatacagcg ggccgaggtg ctgatattgc tgtgccatat cgaggtattt tgcgctaccg
    28021 agggtgtcca gccagcggaa cgcgccgccg tggaacggag ggaagcccag gccgtagacc
    28081 agcgccatat ccgcttccgc cggagtggcg ataatgccct cttccagaca gcgcaccact
    28141 tcgttgacca tcgggatcat catgcgggcg ataatctctt cttcgctgaa atcgcgcttc
    28201 ggttggctca cttctgccag caggtcttca acggcggcgt cttcttcttt cttcggctta
    28261 cctttgctgt cttctttata acgccagaaa ccgaggccgt tcttctgacc aaagcggttg
    28321 gcatcaaaca gcgcgtcgat ggcatcgcgg taatctttct gcatccgctg cgggaagcct
    28381 gctgccatga cagcctgagc gtgatgcgcg gtatcaatgc ccacaacgtc cagcagatat
    28441 gccgggccca tcggccagcc aaactgtttt tccatcactt tgtcgatctt gcggaaatcc
    28501 gcgccgtcgc gcagcagctg gctgaaaccg gcgaaatacg ggaacagcac gcggttaaca
    28561 aagaagccgg ggcagtcgtt aaccacaatc ggcgtcttgc ccatcttgct cgcccaggcg
    28621 acaactttcg cgatggtttc gtcggagctt ttctcgccgc gaataatttc taccaacggc
    28681 attcggtgga ccgggttaaa gaagtgcatc ccgcagaagt tttccgggcg ttccagcgcg
    28741 ttggccagtt cgctgatagg aatggttgaa gtgttagacg ccagcacggt atcctggcgt
    28801 actttttgtt cggtttctgc cagtacggct tttttcactt tcgggttttc aacaaccgct
    28861 tctaccacaa tatccacgcg gtcaaatccg gcgtagtcga gcgttgggtg gatcgtggag
    28921 atcacgccag ccagtttcag accatcgatt ttgccgcgct caagctgctt gttcagcagt
    28981 ttcgcggctt cggtcatgcc gagggttaac gacttgtcgt tgatatcttt catgacaacc
    29041 ggcacgcctt tccacgcgga ctggtaagcg ataccgccac ccatgatgcc tgcgcccagc
    29101 acagcagcct gtttcggggt ttcaacgtct ttggtgagtt tcttcgcttt gccttttaca
    29161 tattgatcgt taaggaaaat gccgaccagt gcgcgagctt cgttggtatg cgccagcggg
    29221 acaaaacttt tgttttccag atttaaggct tcttcacgac caaaacgggc cgcagcttca
    29281 atggttttta ctgcggtgat gggggccgga taatgtttcc ccgccgtttg cgcgaccatt
    29341 cctttagcga tggtgaagct catggtggct tcaatcttgc tcagttttag cggttcctgc
    29401 ttcggctgac gtttcgcttt ccagtcgagg tcgccgttaa tggcctggcg taaaatcgcc
    29461 atcgcccctt caatcagttt ttctgctttg actacgccat ccaccagacc gattttcagc
    29521 gcctgatccg cgccgacatc tttaccggcg gcaatgattt ccagtgcact gtcagcgccc
    29581 agcatacgtg gcatacgtac agaaccgcca aagcccggca tgatgcccag tttggtttcc
    29641 ggcagaccga tgcgcagatc cggcgtcgcc agacgataat cggtcgccag cacgcattcg
    29701 cagccaccac ccagcgcata gccgttgacg gcagcaatgg tcggcaccgg cagatcttcc
    29761 aggcgattaa acacgctatt ggcaaagtgc agccactgac ttaactgttc ttcaggaacg
    29821 aggaacaggg acaaaaattc ggtgatatca gcaccgacga taaaggctgc tttgttcgaa
    29881 cgcagcagca gcccttttag atctgattgc tgttccagca cgccgatggc ctcgccgagg
    29941 ctggcgacgg tcgcagtgtc gagtttatta actgaacctg gggcatcaaa taccagttcg
    30001 gcaatgccat cttccagcca gtcaaggtac agggtgtcgc ctttgtaaag catgtcagtc
    30061 tcctgaatcc gcaaggtaat ctggtcgtac cagatgcatc ggagtgtgtg ttttgtgtta
    30121 aaaatatgca aataaaagat taaagaaatg ccgatctgat cacgctcggc agaaatcacg
    30181 ctctggatga acgatgtgct aagatgcgga gacttaaggt caaaaaaaca gaagggtaaa
    30241 aaatggaatc actggcctcg ctctataaaa atcatatagc taccttacaa gaacggactc
    30301 gcgatgcgct ggcgcgcttc aagctggatg cattacttat tcactccggc gagctgttca
    30361 acgtttttct cgacgatcat ccctatccgt ttaaagtgaa cccgcaattc aaagcgtggg
    30421 tgccggtaac tcaggtgcca aactgctggt tgctggtgga tggcgtgaac aagccgaaac
    30481 tgtggttcta tctgccggtt gattactggc acaacgtcga accgctgccg acctccttct
    30541 ggactgaaga tgtggaagtg atcgcgctgc cgaaagccga tggcattggt agcctgctgc
    30601 ctgctgcgcg cggcaatatc ggttatatcg gtccggtgcc ggaacgtgcg ctgcaactgg
    30661 gtattgaggc cagcaacatc aacccgaaag gggtgatcga ctacctgcat tattaccgct
    30721 ccttcaaaac cgagtacgaa ctggcctgta tgcgtgaagc gcagaaaatg gcggtcaatg
    30781 gtcatcgtgc ggcagaagaa gcgttccgtt ctggcatgag cgagttcgat atcaatattg
    30841 cctatctgac cgcgaccggt catcgtgata ccgacgtacc ttacagcaac attgtggcgc
    30901 ttaacgaaca cgctgcggtg ttgcattaca ccaaactgga ccaccaggca ccggaagaga
    30961 tgcgcagctt cctgctggat gccggggccg aatataacgg ctatgcggct gacctgaccc
    31021 gtacctggtc ggcaaaaagc gacaacgact acgcacagct ggtgaaagac gtaaatgatg
    31081 aacaactggc gctgatcgcc accatgaaag caggcgtcag ctatgtggat taccacatcc
    31141 agttccatca gcgcatcgcc aaactgctgc gtaaacatca aatcatcacc gatatgagtg
    31201 aagaagcgat ggtcgaaaac gatctcaccg gaccgtttat gccgcacggt attggccatc
    31261 cgctgggcct gcaggtgcat gacgtcgccg gttttatgca ggatgatagc ggtacacacc
    31321 tcgcggcacc ggcaaaatat ccgtacctgc gctacacccg tattctccag ccgggcatgg
    31381 tgttaaccat cgaaccgggt atctacttca ttgaatcgct tctggcaccg tggcgtgaag
    31441 ggcagttcag caagcacttc aactggcaga aaattgaagc actgaaaccg ttcggcggca
    31501 ttcgtatcga agacaacgtg gtgatccacg aaaacaacgt ggaaaacatg acccgggatc
    31561 tgaaactggc gtgatggaaa gctggttaat tcctgcggca ccggtcacgg tcgttgaaga
    31621 gatcaaaaag agccgcttca taacgatgtt ggcgcatacc gatggcgttg aggcggcgaa
    31681 agcgtttgtt gaatcggtgc gggcagaaca ccccgatgcc cgccaccatt gcgtggcgtg
    31741 ggtggcgggt gcgccggatg attctcaaca gctgggtttc tctgacgacg gggagccggc
    31801 gggaacggca ggtaaaccga tgctcgccca gctaatgggc agcggcgtcg gggaaattac
    31861 cgctgtggta gtgcgctact acggcggcat attgcttggc accggtgggt tagtgaaagc
    31921 gtatggcggc ggcgtgaatc aggcgctgcg ccagctaacg acccaacgca agacgccatt
    31981 aaccgaatat actttgcaat gtgaatatag tcagttaacc ggcattgaag cgttgctggg
    32041 gcagtgtgac ggcaaaatta tcaacagtga ttatcaggca ttcgttctgc tgcgggtggc
    32101 gcttccggcg gcgaaagtgg ctgaattttc cgcaaagctg gcggatttta gccgtggttc
    32161 attgcaattg ttagcgattg aagaataatc cccacttcgt tttgcagaac taaggaagcg
    32221 gcagagatgc attttcgcgc cattacccga atcgttggac tactggtcat cttattttca
    32281 gggaccatga ttatccctgg gctggtagca ctcatctacc gggatggagc gggccgcgct
    32341 tttacccaga ccttttttgt cgccctcgcc attggctcta tgctgtggtg gccgaaccgc
    32401 aaagagaagg gcgaactgaa atcccgtgag gggtttctga tagtggtgct gttttggacc
    32461 gtgctgggta gtgtcggtgc gctccctttt atcttctcgg aaagcccgaa cctcacgatt
    32521 accgatgcgt tttttgaatc tttctctggc ctgaccacca ccggggccac tacgctggtg
    32581 gggctggatt cgctccctca tgccatcctc ttttatcgcc agatgctgca atggtttggc
    32641 gggatgggga tcatcgtgtt agcggttgcg atactgccta tcctcggcgt gggtgggatg
    32701 cagctctatc gcgcagaaat gcccggcccg ctgaaagata acaaaatgcg cccgcgaatt
    32761 gcggaaacgg cgaaaactct gtggttgatt tatgtcttgc tgaccgtcgc ctgtgcgctg
    32821 gcgttgtggt ttgccggaat ggatgccttt gatgccatcg gccatagctt tgcgactatc
    32881 gctattggcg gcttctcgac acatgatgcc agtatcggtt atttcgacag tccgactatt
    32941 aacactatca ttgctatctt cctgctgatc tccggctgta actacggtct gcacttttca
    33001 ctgttaagtg ggcgtagtct gaaggtttat tggcgcgatc cggaatttcg catgtttatc
    33061 ggcgtacagt ttacgctggt ggttatttgt acactcgtac tgtggtttca taatgtctac
    33121 agttcggcgc tgatgacaat taaccaggcg tttttccagg tggtatcgat ggcgacaacc
    33181 gccgggttta cgactgacag cattgcccgc tggccgctct ttttgccggt actgctttta
    33241 tgttcagctt ttatcggcgg ttgcgccggg tcaacgggcg gtggcctgaa agtgatccgc
    33301 atcctgctgc tgtttaagca ggggaaccgt gagctgaaac gactggtgca tccgaacgcc
    33361 gtctatagca ttaagctggg gaatcgcgca ctgccggaac gtatcctcga agccgtttgg
    33421 ggatttttct ccgcctatgc attggtgttt attgtcagta tgctggcgat tatcgccacg
    33481 ggcgtggatg atttttctgc ctttgcctca gttgttgcga cattgaataa cctggggcca
    33541 gggcttggcg tggttgctga taactttacc agtatgaacc cggtggctaa atggatcctg
    33601 attgccaaca tgctgtttgg tcgtctcgag gtctttacat tactggtgct ctttaccccg
    33661 actttctggc gtgaatgatg gagtaatacg tgaaaacatt aattcttttc tcaacaaggg
    33721 acggacaaac gcgcgagatt gcctcctacc tggcttcgga actgaaagaa ctggggatcc
    33781 agacggatgt cgccaatgtg caccgcattg aagaaccaca atgggaaaac tatgaccgtg
    33841 tggtcattgg tgcttctatt cgctatggtc actaccactc tgcgttccag gaatttgtca
    33901 aaaaacatgc gacgcgactg aattcgatgc cgagcgcctt ttattccgta aacctggtgg
    33961 cgcgcaaacc ggagaagcgt actccacaga ccaacagcta cgcgcggaag tttctgatga
    34021 actcgcaatg gcgtcccgat cgctgcgcgg tcattgccgg ggcgctgcgt tacccacgtt
    34081 atcgctggta cgaccgtttt atgctcaagc tgattatgaa gatgtcaggc ggtgaaacgg
    34141 atacgcgcaa agaagttgtc tataccgatt gggagcaggt ggcgaatttc gcccgagaaa
    34201 tcgcccattt aaccgacaaa ccgacgctga aataagcata aagaataaaa aatgcgcggt
    34261 cagaaaatta ttttaaattt cctcttgtca ggccggaata actccctata atgcgccacc
    34321 actgacacgg aacaacggca aacacgcagc cgggtcagcg gggttctcct gagaattccg
    34381 acagagaaag caaaaataaa tgcttgactt tgtagcggga aagcgtatta tgaatacccc
    34441 gcgccg
//
LOCUS       DAEBXN010000036        33737 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.036, whole genome shotgun sequence.
ACCESSION   DAEBXN010000036 DAEBXN010000000
VERSION     DAEBXN010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33737
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.036"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            376..815
                     /locus_tag="KJL99_001776"
                     /pseudo
     CDS             376..815
                     /locus_tag="KJL99_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(894..2591)
                     /gene="treA"
                     /locus_tag="KJL99_001777"
     CDS             complement(894..2591)
                     /gene="treA"
                     /locus_tag="KJL99_001777"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HBD4436527.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNVKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     NYFTMLGLAESGHWDKIADMVANFAHEINTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDSTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATHPTVKSATTQPSTKEAQPTP"
     gene            complement(2911..4329)
                     /gene="dhaM"
                     /locus_tag="KJL99_001778"
     CDS             complement(2911..4329)
                     /gene="dhaM"
                     /locus_tag="KJL99_001778"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HBD4436528.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRHLAEDNFGETEEVAPPTLRPVLPVSGKAFYYQPVLCTVQAKSTL
                     TAEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLGDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            complement(4340..4972)
                     /gene="dhaL"
                     /locus_tag="KJL99_001779"
     CDS             complement(4340..4972)
                     /gene="dhaL"
                     /locus_tag="KJL99_001779"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HBD4436529.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            complement(4983..6053)
                     /gene="dhaK"
                     /locus_tag="KJL99_001780"
     CDS             complement(4983..6053)
                     /gene="dhaK"
                     /locus_tag="KJL99_001780"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HBD4436530.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLEHGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            6281..8200
                     /gene="dhaR"
                     /locus_tag="KJL99_001781"
     CDS             6281..8200
                     /gene="dhaR"
                     /locus_tag="KJL99_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HBD4436531.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKLAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            complement(8300..11167)
                     /locus_tag="KJL99_001782"
     CDS             complement(8300..11167)
                     /locus_tag="KJL99_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBD4436532.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSSSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNRGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPAPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLY
                     IDSTHASPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNG
                     VNANGTANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNDSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(12012..13103)
                     /gene="ychF"
                     /locus_tag="KJL99_001783"
     CDS             complement(12012..13103)
                     /gene="ychF"
                     /locus_tag="KJL99_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HBD4436533.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(13220..13804)
                     /gene="pth"
                     /locus_tag="KJL99_001784"
     CDS             complement(13220..13804)
                     /gene="pth"
                     /locus_tag="KJL99_001784"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBD4436534.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGSEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            14082..14360
                     /gene="ychH"
                     /locus_tag="KJL99_001785"
     CDS             14082..14360
                     /gene="ychH"
                     /locus_tag="KJL99_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HBD4436535.1"
                     /translation="MKRKNASILGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(14415..16094)
                     /gene="dauA"
                     /locus_tag="KJL99_001786"
     CDS             complement(14415..16094)
                     /gene="dauA"
                     /locus_tag="KJL99_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HBD4436536.1"
                     /translation="MNKIFSSHVMPFRALIDVCWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(16219..17166)
                     /gene="prs"
                     /locus_tag="KJL99_001787"
     CDS             complement(16219..17166)
                     /gene="prs"
                     /locus_tag="KJL99_001787"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HBD4436537.1"
                     /translation="MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(17317..18168)
                     /gene="ispE"
                     /locus_tag="KJL99_001788"
     CDS             complement(17317..18168)
                     /gene="ispE"
                     /locus_tag="KJL99_001788"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HBD4436538.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(18168..18791)
                     /gene="lolB"
                     /locus_tag="KJL99_001789"
     CDS             complement(18168..18791)
                     /gene="lolB"
                     /locus_tag="KJL99_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HBD4436539.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            19005..20261
                     /gene="hemA"
                     /locus_tag="KJL99_001790"
     CDS             19005..20261
                     /gene="hemA"
                     /locus_tag="KJL99_001790"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HBD4436540.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFVDSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            20303..21385
                     /gene="prfA"
                     /locus_tag="KJL99_001791"
     CDS             20303..21385
                     /gene="prfA"
                     /locus_tag="KJL99_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HBD4436541.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            21385..22218
                     /gene="prmC"
                     /locus_tag="KJL99_001792"
     CDS             21385..22218
                     /gene="prmC"
                     /locus_tag="KJL99_001792"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBD4436542.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            22215..22607
                     /locus_tag="KJL99_001793"
     CDS             22215..22607
                     /locus_tag="KJL99_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HBD4436543.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            22611..23420
                     /locus_tag="KJL99_001794"
     CDS             22611..23420
                     /locus_tag="KJL99_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HBD4436544.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDGWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVERCPEDPISEMIRAQINNIAHKHIVLH"
     gene            23456..24310
                     /gene="kdsA"
                     /locus_tag="KJL99_001795"
     CDS             23456..24310
                     /gene="kdsA"
                     /locus_tag="KJL99_001795"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HBD4436545.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(24459..24566)
                     /locus_tag="KJL99_001796"
     CDS             complement(24459..24566)
                     /locus_tag="KJL99_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HBD4436546.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIIAAAIVSWWRNRK"
     gene            complement(24971..26071)
                     /gene="chaA"
                     /locus_tag="KJL99_001797"
     CDS             complement(24971..26071)
                     /gene="chaA"
                     /locus_tag="KJL99_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HBD4436547.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLVLVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQAKTHQSLFVYEHEDDSDDDDPHHGKTSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            26341..26571
                     /gene="chaB"
                     /locus_tag="KJL99_001798"
     CDS             26341..26571
                     /gene="chaB"
                     /locus_tag="KJL99_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HBD4436548.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            26729..27424
                     /gene="chaC"
                     /locus_tag="KJL99_001799"
     CDS             26729..27424
                     /gene="chaC"
                     /locus_tag="KJL99_001799"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HBD4436549.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(27468..27821)
                     /gene="ychN"
                     /locus_tag="KJL99_001800"
     CDS             complement(27468..27821)
                     /gene="ychN"
                     /locus_tag="KJL99_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HBD4436550.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            28006..29400
                     /gene="ychO"
                     /locus_tag="KJL99_001801"
     CDS             28006..29400
                     /gene="ychO"
                     /locus_tag="KJL99_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HBD4436551.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLQDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(29401..30051)
                     /gene="narL"
                     /locus_tag="KJL99_001802"
     CDS             complement(29401..30051)
                     /gene="narL"
                     /locus_tag="KJL99_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HBD4436552.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(30044..31840)
                     /gene="narX"
                     /locus_tag="KJL99_001803"
     CDS             complement(30044..31840)
                     /gene="narX"
                     /locus_tag="KJL99_001803"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HBD4436553.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            32179..33570
                     /gene="narK"
                     /locus_tag="KJL99_001804"
     CDS             32179..33570
                     /gene="narK"
                     /locus_tag="KJL99_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HBD4436554.1"
                     /translation="MSHSSASERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATGTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
BASE COUNT         8289 a         8338 c         8588 g         8522 t
ORIGIN      
        1 cattttatta atgcatttct cattcgggtg aaatttccgc tacctggacg cataaaaccg
       61 gaactctcgc caaaagtcgg gcactccgaa ggaattgacg cgttgcgctg ctctgtaacg
      121 gcatttaccg caagccattt atttcgccag cgacaatccg cagagaacca aacgcatcag
      181 gaaacgactg aacacaataa atactgtatt gatcaacata ttatactggc acctccagca
      241 cgcagtgagg atacactgta tcatcagcga atcttatcac tgcggagctg gttgaacgac
      301 tttctgagcg aaataagtcg gttcccggta acaacaacag aatccttttt taattattgt
      361 ttcgttgttt ttggtgtgat tcagaaaata aatattttag acagtagcca gtcaggcgtt
      421 aaaaatgggg cggatagtga ggcggttttg gctgcgctta ttgaacattt agaattgatc
      481 aatccttctg gcagattaag ccaaaatacc cgttcggcaa tgcttcagtt acgcgaagag
      541 gaatggtctg aattcttttt ttggttgcta aattctctgg agtgtctgga ttatgtgatt
      601 attaatttaa ctccagaaag caaaaaaacg ttgatgagtg aacaccgtaa caatattcgg
      661 tagcaattga tgctttatat agccaacgtc ggcgtaaatc gccgggtgat gaaagtgaaa
      721 cacttacccg tagaaatgac gctatcttcg gcaaccacgt atggcaaaca tttgcgcaat
      781 acttcccgcc aggacttgaa aaaccgtctg tgtagaaaaa accatagata tggtgttatc
      841 tgccgacaac gccatccgga gaagccgaat gacgttgtcg gagtaagcgc tggttaaggt
      901 gtgggttgtg cctctttggt tgagggttgc gtcgttgctg acttaacggt cggatgcgtc
      961 gccggaacat tgtcacacgg ttgctctttc gggcagatca aatccagcat tttcagcgtc
     1021 acgccattgg tccagccaaa gccatcctgt aatggatatt cgccaccgcc gccccccgtt
     1081 ccggtggtgc tgacatcata tttttccacc agctttttct cccggtcata ggtgtgctga
     1141 acattggtca ggaagtgcca gctaatgtcc atcgccacct ctttttgccc gtagttttgt
     1201 aatccttctg tcgcgaccca ctgtaacggt gcccagccat ttggcgcatc ccattgttgc
     1261 ccacttttca ccgacgtggt gttcaggccg ccgggttgca gcagatgtgt tttcgtcgcc
     1321 gtcgccattt tgttggcgcg atctttcgct gccgcattga cgtacagcgg gaacagggcg
     1381 gccgcggtta actgattgcg cactttatga cttttcaggt cgtaatcggc ataccagcct
     1441 tgttgatcgt tccacaggta tttttcgatc cctttttgac gggcattcgc cagcgtttcg
     1501 tactggtttg ccatcgcgtt atctcctgca gctttgctgg cgcgggcgag gattttttcc
     1561 attttaaaca tcaggctgtt cagatcgacc ggtacgatgc tggtggtgcg taaggtattt
     1621 aactgctgcg ggttgtccat ccagcgcgag ctgaaatccc agccagacgc agcggcagag
     1681 cgcaggtcgc ggtaaatttc agtggcaggt cgattcggat tgcttttggc ggtggcaata
     1741 tcttccaccc atgactctgg tcgtggcgta tcgcgatcgt cccagtagcg gttgagaagg
     1801 gtactatcct gaagttttac aacgcgtttt tcctgttgtc cggcttgcag gttttcaaca
     1861 ccgtccatcc agtaagcata ttctttttgc atttgcggca ggtattgctt caacgcggca
     1921 tcgccttcat gctgcgccag taactctacc atcagggcaa agaagggcgg ttgcgagcgg
     1981 cttaaatagt aactgcggtt gccgttggga atatgaccgt aagtgtttat ttcatgagca
     2041 aaattggcca ccatatccgc gattttatcc cagtgaccac tttcggcaag tcctaacatg
     2101 gtgaagtaat tgtcccagta atatacctcg cgaaaacgtc cgcccggcac gacataaggt
     2161 tccggcagcg gtaacagaga atcccatttt tcggtgtttt cggtagaacg cgttaatacc
     2221 ggccaaagtc cgtcaatatg ttcgcgcagt gactgcccct ctggcggaac atatttctcg
     2281 ccttctttcg gcagggtgaa attgacgtta acgaaatggc gcagatcaaa tccgctctgg
     2341 ttttgctgca tccgataatc agcaaggatc atcagcggat cgctgttagg cacggcatcg
     2401 gcaaaggttt tttggtccgg aaaaagtttg acgttttgca catcattaaa cagcggccct
     2461 aataaaatat caggcggctg tggtgttacc ggtgtttctt ctgcctgcac cgatagcgca
     2521 gcgaaacaca aaaagataca ggctggaatt aacgccattt tttgcgggcg agaaggtgcg
     2581 ggggatttca tcaatcattc tcctttggcg aaaccgaata aaacggtatt caagtatcag
     2641 aaaaccttag ttcaggatcg cactagctgc atgatcgaaa tctcattctg cgaacggtca
     2701 ggcattttag aaaagggaat taaagggata aataacggcg aaatagagtg atcaaataac
     2761 gtctgtttat tagccagcca gcgacatttg gttcgcgatt agcgaagaaa aatcatcata
     2821 aattggtaaa aaatataaca ggtagttaaa aaacatcagt gctgggtaaa ttgccggatg
     2881 acatcagaac gatgccatcc gaacggtggc ttaaccctga cggttgaaac gttgcgtttt
     2941 aacgtccagc gttagcgttt cttctggttg tatcgcatac agtttctcac cctgctggca
     3001 aatccagcca atccccagtt cacgggcgat tagggcgctg tgggataccg gacttccggc
     3061 gctaaggcag atacctttta caaccgccgg atccagttgc agtactgtgg aaggataaat
     3121 gttctccgcc agtagaatag ttggcgagtt aaactgcggg agttcttctt tcgtttgggt
     3181 caggtggacc agggtgcgat gcagaagatc gtccacatca atatagcgag cttgtagata
     3241 ttcatcatcc agttgctggt attgctggct aagttcttta agaacttgct gccaggcata
     3301 ttctgccgtg caatgttcat gctgaaggag ttcgcttgcc gccgccagca gttccggatc
     3361 acctaacagt gtatggtgac cagaaaagat tgcggcaata tcgtcaagcc cgctggcttc
     3421 tgctttcgct gttaacgtca tcagatctaa taacgtgaag tcaatagcct ggcgtaatcg
     3481 atcttgttct tcttccgcgg tcagggttga ttttgcctgt accgtacata aaactggttg
     3541 ataataaaag gctttacccg aaacaggcag aacgggacgc agagtaggtg gagcgacttc
     3601 ctccgtttca ccaaagttat cttcagccag gtgacggaaa gcgatcagtg cctcttcagc
     3661 ttctggccct ttcgcaatca ggcgcagcgt atcgttatag cgaacttgta gtaacgcaat
     3721 ctggttaata ctctctggtg tgacgcattt gccgtttttt tccagcaaca tatcggcatt
     3781 aaatgtcgat aaggtataaa ccagccggga ggccggacgt acatgcaggc cgttacggtt
     3841 ttttatgacc accgccagag aacgggcttc ttcatcgtac ggaggacatg tgtcagagat
     3901 ttcagtgtcg gaggacggta aacccagttg ttcacgtttg gcttccagcg catgcatagc
     3961 gtcaaagata actttgtcga tatccgcccc cgaggccgcg ctgaccgttg ctgccagtgt
     4021 accttcgacc aacggcgcag cacacaaacg tacttttgcg gcgatctcgg gagccagcaa
     4081 ttccagcgca gtttcagcac tcaataatgc gctacccata tccatcatga ccagcacatg
     4141 gtcggcatca gcaacagatt cgatggcctc catcactttg acggcatcgg taccaatggg
     4201 attttgtgga tcgtcaattc ccgcggcaat ggcgatttta caactatcac tcattaacat
     4261 ctgacgggct aattcaccga caccttctcc cagtcggctg ctatgtgaaa ctatgaccag
     4321 gtttaccatc accaattcct tactcttttg cggctaacgc caacatttgc atcataaaca
     4381 tcaccgaggt cgcgccggga tcctggtgac caatactgcg ttcaccgaga taactggcgc
     4441 ggcctttgcg ggcttgcatc gtaatcgtac tttgtgcagc ggattcggcg atgctactgg
     4501 cagcttcgag cgccaccgga acagagagat tttgctcgct ggactgacgt aacgattcca
     4561 ccaccggcac ccacacatca cacatggttt tatcgccagg ttcggctttc ccgcgactga
     4621 ttacgccgtc cgcgccatcg cggaacatct gataaagctc ttccagtgtc aggctttgcc
     4681 gtgcctgggt cgcctgtgcg gcgcggataa agaaggtacc gaacagcgga ccactggcac
     4741 caccgacgct ggaaagcagc gtcataccgg tatttttgag aatgaaaccg atatctttat
     4801 ctgcgatagc agggagtttt tccaccactt tgctaaagcc tcggttcata tttagcccgt
     4861 ggtcagcgtc gccaatctcg cgatccagcc cggtaagata ctcgctctcg gtgttgaaaa
     4921 tatcgccaca acgagtgagc cagttaacaa tttgagttct gctcagtgac attgctttct
     4981 ccttatttac cccagttaag ggccggggtg tggaccgggg cgtcccagag agccagcgtt
     5041 tcgtcatcaa ctttcagtaa ggtgattgag aaaccggtca tatccagtga ggtgcagtac
     5101 gcgccaatta aattacgttc gatagtcaat cccgcttgct ggcaacgtgt ggtcaggcgg
     5161 ttatagacgc cgtacagctc agaaagcgga gttgcgccaa gattgttaac cagtgcaatc
     5221 acccgatcgc cagactggag cggttgtttg gtttgtggtt cttcctgcca gctgccttgt
     5281 tgataatccc agaaacgcaa agtgcgatgg tatgagccat gttccagcag ggtgtcgaac
     5341 atttcatcga cggtttgatc aagggaagag aaagggcggc ggtcaatacc tggctcacca
     5401 tgaatgccta cgccaaactc catctcatta tccgcaaggg taaaagaagg tttgcccgcg
     5461 gcaggaacgg tacaggcacc gagagcgata cctattgagt ggccttgatt attcaactta
     5521 cgccccagtt ccgcacaggc gtccagtgag tcgccacgct ccgccgctgc gcctacgagt
     5581 ttttcaatta atacggtgtt ggcaacgccg cgtcgcccgg cggtataaag actgtctttt
     5641 accgcaacgt cgtcatcaat gaccacagtg gtcactttta cgccgctatc gtgcagtaac
     5701 tcggtcgctg tttcaaagtt aagaatatcg ccggtgtaat ttttgataat caacagtaca
     5761 ccttcgccgc catcaatttg catggagcat tcaaagattt tatcgggcgt cggtgaggtg
     5821 aaaatttcgc ccggacaggc ccccgaaagc atcccctgac cgatataacc acagtgcatc
     5881 ggctcgtgtc cgctgccgcc acccgacagc agggcgactt ttcctgcaac aggggcatca
     5941 gctcgggtga catacaccgg atcctgatgc agtgtcagcg atggatgcgc tttcgccagt
     6001 cctgccagtt gttcgtccag tacgtcttgc acatcattga tcaatttttt cattattttg
     6061 ctccagcaat tacggtaggg catggatgat gttcaacgac acggcgacca ctgactgccg
     6121 atgaatccat tgtgcatcag gcaagggaaa agaaaattag cccaaattat gtttcatagt
     6181 gaaacatatg ctttaatgaa tgttccatat tgaaactttt acgtgtatta atacttaaaa
     6241 ttgcgagccg gaacaccttt tgtcataagg gatgcgggat atgagtggcg cttttaacaa
     6301 cgatggtcgg ggcatatctc ccttaattgc aacctcctgg gagcgatgca ataagctgat
     6361 gaaacgggag acatggaacg taccacatca ggcccagggc gtgacatttg cttctattta
     6421 tcggcgtaag aaagcgatgc tgacgctcgg gcaggctgcg ctggaagatg cctgggaata
     6481 tatggcaccg cgagagtgtg cgctgtttat cctcgatgaa accgcctgca ttctcagccg
     6541 taatggcgat ccgcaaacct tgcagcagct aagtgcactg ggattcaatg acggcacgta
     6601 ttgcgccgag ggaattattg gtacttgtgc gctatcgtta gcggctatct ctggtcaggc
     6661 cgtgaaaacg atggccgatc aacatttcaa acaggcactc tggaactggg ccttttgtgc
     6721 aacgccgttg tttgacagca agggccgatt gacgggaaca atagcgctgg cgtgtccggt
     6781 tgagcaaact accgcagctg atttgccgtt gacgttggca atcgcccgcg aggtcggtaa
     6841 tttactgctg acggacagtt tgctcgctga aactaaccgt catttaaatc aacttaatgc
     6901 cctgttagaa agtatggatg atggcgtgat tagctgggac gagcagggta atttgcaatt
     6961 tattaatgcc caggcggcgc gggtcttgcg ccttgacgcg acggcaagtc agggacgggc
     7021 aatcactgaa ctcttaacgt tacccgccgt attgcaacaa gcaataaaac tggcacatcc
     7081 gctcaaacac gtagaagcaa cctttgaaag ccagcaccag tttattgatg cggtgataac
     7141 ccttaaaccg ataatagaaa cgcagggaac cagctttatt ttgttgctcc atcctgtgga
     7201 acagatgcgg cagttaatga ccagtcaatt aggaaaagtc agccatacct tcgctcatat
     7261 gccacaggac gatccgcaaa cccgccgctt gattcatttt ggtcgccagg cggcgcgcag
     7321 tagctttcct gtcctgcttt gtggagaaga gggcgtgggc aaggcactgc taagtcaggc
     7381 aattcataat gaaagcgagc gtgctgcagg tccttatatc gccgtcaatt gtgagttata
     7441 tggtgatgct gcgctggcgg aagaatttat tggtggcgat cgcacggaca atgaaaatgg
     7501 ccgtctgagt cggctggaac tggcacacgg cggcacgctg tttcttgaaa agattgaata
     7561 tctggcggtg gagttacagt ctgctttgct gcaggttatc aagcaggggg ttatcacgcg
     7621 actggatgcg cggcgtttaa taccaattga tgtcaaagtg attgcaacaa cgaccgcgga
     7681 cctcgcaatg ctggtggaac aaaatcgttt tagtcgccag ctgtattacg cgctgcatgc
     7741 atttgaaatt accatcccgc ctctgcgtat gcggcgtggc agcattccgg cgctggtgaa
     7801 taacaaatta cgcagtcttg aaaaacgctt ctctacgcgg ctgaaaattg atgacgatgc
     7861 cctcgctcgc ctagtttctt gtgcatggcc aggcaacgat tttgaacttt acagcgtcat
     7921 cgagaatctt gctctgagta gtgataacgg gcgcattcgc gtcagtgatt tacctgaaca
     7981 tctgtttacc gagcaggcga cagatgatgt cagcgccacc cgcctttcca ccagtctgtc
     8041 atttgcggaa gttgaaaaag aggcaattat taacgcagcc caggtcacag gcggtcgcat
     8101 tcaggaaatg tcggctttac ttgggatcgg ccgcactacg ctgtggcgga aaatgaagca
     8161 acatggcatt gatgcagggc agtttaagcg ccgggtatga aagacagaaa cgatttctga
     8221 tacatcagag tgatctgtat ttcattccgg cgcacgctaa caattttcag catcgtttaa
     8281 gggcttgtct atcccgcact taaaagctga agcgatatcc tccgttgact tgcttctgat
     8341 caaagaggtt accctgcgtg taatccgctt cgagatagaa tgtgtgctgt ttgttatact
     8401 gtgcactgac tcccacgccg ttattccagc cattaccttt gaaactgtac ttctcccggg
     8461 agtcgttcaa cagatattcg gtatcccctg aaaactcgcg gatagcgcca gtcttcacat
     8521 agacattcag ctggctgttg cctgcggtga tgtcataccc cagtatcatg ctggcacgcc
     8581 ccagcagcga ttcgtagtga ttcagatgta tattgaggcc attactcgcc ttcatagcca
     8641 tctcattctg gtggctgtat gtaagctggg tttgcggctc tatatagaac ccataaccag
     8701 taggggacag gttgaacctc tgcccggctt ccagggagat gctcattcca ttcgcagtgc
     8761 cgttggcgtt aacgccgttg ttctgactgt ccagtacgtg gaaactattt ttctggcgcg
     8821 atgcttttat aacgagatcg ctgtaaaaac cgttttgtgc catgtaactg gcgtacattc
     8881 ccatgtagtc tgaacgtgcg gtaccgtcgc ctccgctata gtccggcgat gcatgtgttg
     8941 agtcaatata cagaccgaca tacaacggca ttacatcaga gagacgttta tccccaccaa
     9001 actggatacc gctgtaaccc atgtcaaagc cgctcagttt gccactggca aaggagtcca
     9061 ggcttccccc ataactgcgc aaccagatat taccgtcttt actctgattt cgcaagtcac
     9121 ccatccgttg catcagcgta cggttttcaa catagttcaa taagtaaccg acattgagat
     9181 aattgccgcc agcatctgcg gtcgtggtgg gtttaggcgt gggagcgggt tcaggcgtag
     9241 gatcggggtt gactatggga ggctgagcgg gagctggtgt gggttcagga ttaggagtcg
     9301 gttccggaac tgtcccggaa gcgtaaagct cccagttagt gccattttta cgcacatcgt
     9361 acagatatcc ccccaactca acctgagaag acgcgctgaa cgaggccgcg ccgtcagtgg
     9421 tttttaccac tgtcagaact tcatttcccg ttgtggcctc gctgccctgg ttgcggatag
     9481 ccaatacgtg attaccagca ctactcccgc tgatattcaa taaatcccct ctattattaa
     9541 cgccattacc ctcgccaaca acatcagcac gcataataaa ggtactgtta ccgctcaggt
     9601 tctctacgtt taatgtggta aatgtgccgg cagttgaccc gtggctggca aaatcgacag
     9661 ttgaatggct cagcgccagc gtgtcgagat tagaattact tgttacgttc cagacgctgt
     9721 tattcattgc aacgtccagt ttcccgccat tgacattatc gctgagggag gaacctgtcc
     9781 aaaccgaccc agggtgcata tccagattga ttagcccacc tttggaaaga acgctaccgt
     9841 tgataagcat acgacccgag gcgttgatgc ggctggcggc ataaccatcg tcatgttgcg
     9901 ttgcgatggc catctggtcg ggtgtactca tatcaatgac cagatcgctc gtgaggtcga
     9961 tctggctgtt ggtcatggca taaatccctc tggctcctgc cgcgccggtg atagccaaac
    10021 tgtctccggt tattctaccg ccgctgagcg cccacaaacc cagcgccaga ctgccattac
    10081 gatcaaccgt aatatcggta ttttgcatgt tgataacagc cgttgccgtc tgggccgagg
    10141 caccataaga accgccggaa aagatgctgt ttcgttgcgc tgccgtgcca gaaaaattga
    10201 ttgtcgcgtc tgaactactg gtgacgaggc caccgccctg cgcggaagaa atatggctat
    10261 ctgcaccgat agtggttgta ccaccacgaa cttcgacgcc attggccgcg gctccagtta
    10321 catcaacagt gagtgcattc gcgctaacct ggccaaagct ccagaggccg tgagcattat
    10381 cgccattggt tttaatggta ctgtttgttc cgagatcgac aacagagttt ttctgtacgt
    10441 ttatccccat ggcgctgtag ccctgaacat cgattgtcag gtctgtcgcc gtaaaacgcg
    10501 cagcaccatt ggcgttattg ccgttgagac caccgatata aacacctgta cttccatcgg
    10561 tcttgatttt acttccgctt ccaagatcga cactggtgcc atagtcattg atggttaggc
    10621 ctataccgtt cgagttttca atggtgaatt gagtggctgt tagtgttgag ctatgcccaa
    10681 taatgatgcc gtcatcgtta ctcttaatgg tgctgcctgt gcctaagtca gcatgggtat
    10741 agtcaccaat taagttaatg ccgatggcag aggtttgacc aacaacatct actgtgagtc
    10801 ggttagctgt cagggagctg tttttgccct gaataatgat tccttttgca tatccagagg
    10861 cgtcgttgac attgacggtg acatcattac ccaggttgat attgccaggc gtgtttcctg
    10921 ctggggtcat tacaccatag aggtttcctg atggatcggc ggtatctcct gtaattctgt
    10981 caccatcatt aatagtaata ttggtgccat attgacttcc tgtatcagca gctatcagca
    11041 ggggggaaaa gataattgca ttcaaaccaa tagcgccaaa ttttatcagt tgaattgagg
    11101 gcgaacggat ttttaattca gcgagacgat cggctttggt attcccattg tgttgtttga
    11161 tgcccatacc actcctatat agtacccagg ctatgggtct atggattttg tcattttgtt
    11221 taagtcaatg aataatatcc tcttatcatc atgatatctg tatggtgcaa tcatgatgag
    11281 cttctctgag aataattctc tattaccggg caaaatcagg aacacaggaa ttaatatata
    11341 tttaaccccc ccccttacaa aggtgagttt atatcgagtt attccctaag ttgtcgttac
    11401 ttattccaat tcgttactat gaatatggca aaaatattac aacaaacagc ggttaaaaaa
    11461 tttggtgaag attttttgta ataatcctaa ttagttatgg gattttcgct tgatgtatct
    11521 gcataacaaa aacaatatgc gccatttttg cattactaat aagaaaaagc aaaaaacatc
    11581 gatattcatt tttgttcaat ggattttctg gcctatcgtt attattgtgc tactggttat
    11641 cattttcatc ttgccaggat cgcacgtaat cacgttattc ggggacttta tctgcgggat
    11701 attacccttc aggtaagtcc ttttctcgcc agcatattgg agcgagatca atattgatca
    11761 gctgtgaaga gggctgatgt gtagtggaac aaaaactgac gttatatcac tgctaatgag
    11821 atatcagtca tctgaacact tatgtcttta attattctcg tggttcactg taggcaataa
    11881 gcacaaaagt gtaggatgtt acaagaatga ttaggactcg gtgaaatgaa aaatccacgc
    11941 aattgcgtgg attttatata cttttgcgct cttcatgaga tttagcgaaa cctcatgaga
    12001 caataaatta attagacgtt gaaaaggaag ttcatcacat cgccatcttt cacgatgtaa
    12061 tctttacctt ctgcacgcat tttgcctgct tctttcgcgc cttgttcacc tttgtaagta
    12121 atgaaatctt caaacgagat ggtttgtgca cggataaagc ctttttcaaa atcagtatgg
    12181 attttgcccg ctgcctgcgg cgcggttgct ccaaccggaa tggtccatgc acgcacttct
    12241 ttcaccccag cggtgaagta agtttgcagg ttcagcagct tataaccggc acggatcaca
    12301 cggttcaggc ccggttcttc cagcccaagc tcctgcataa actcgtcacg ttcttcgtcg
    12361 tccagttcgg caatgtctgc ttcaacagca gcacaaaccg gaaccacaac agaaccttct
    12421 ttcgccgcga tttcacgcac ctggtcaaga tatgggttgt tttcaaaacc atcttcgttg
    12481 acgttggcta tgtacattgt tggtttcagc gtcaggaagc tcaggtaacg aatagccgct
    12541 ttctcttcag cgcttaaatc cagcgcgcgc agcatacctg cgttttccag ctggggcagg
    12601 catttttcca ggaccgccag ctcagctttc gcgtctttat cgccaccttt ggctttcttc
    12661 tgtacgcgat gaatcgcacg ttcgcaggtg tcgaggtctg ccagcgccag ttcggtgttg
    12721 ataacttcaa tatcgtcagc cgggttaact ttgccggaaa cgtgaatgat gttgtcattt
    12781 tcaaagcagc gaacaacgtg accgatcgct tcggtttcac ggatgttggt caggaactgg
    12841 ttacccagac cttcgccttt cgatgcgcct tttaccagac cggcgatatc gacaaattcc
    12901 atggtcgtgg gaagcgtacg ttgcggtttt acgatttcag ccagttgatc caggcgaggg
    12961 tcaggcattg gtacgacgcc tgtgttcggc tcaatggtgc agaatggaaa gttggccgct
    13021 tcaataccgg ctttggtcag cgcgttgaac agggtagatt tcccgacgtt gggcaaaccg
    13081 acgataccgc atttgaatcc catgatttaa atcaccttaa tatcttaata atcaacctgt
    13141 tattgctaac agattgcaga aatggaaata actttgccta ttatacacgg cactcggcaa
    13201 aaatgccgca gacaacgact tattgcgctt taaaggcgtg caatcggttc gttgctttgg
    13261 tcaagccatc tgtaaaccac atttcagtac aacgcgccgc ttcgtcgatg gcttcatcaa
    13321 ttaacttctg ttcactaaca ggcggtttgc ctaacacaaa accgacaact ttatttttat
    13381 cgcccggatg accgattccg atgcgtaaac ggtgaaagtt agggttatta cccaatttac
    13441 tgatgatgtc tttcagtcca ttgtgaccac catggccacc gcccaactta aatttggcga
    13501 cgccaggagg cagatccagt tcgtcgtggg ccaccagaat ttcgtccgga ttaatgcgga
    13561 aaaaactggc catcgccgca acggctttgc cgctgagatt cataaatgta gtcgggacta
    13621 acaggcggac atcttcgctt ccaagggtga ctcgcgaagt ataaccaaag aatttagcct
    13681 cttcgcgcag cggagcgcgc aaacgctctg ccagtaagtc aacgaaccag gcaccagcat
    13741 tatgtcgcgt tgcggcgtat tcagcaccgg ggttcgccag gccgacaatc aatttaatcg
    13801 tcacgttttt ttgtcctgag tgtgtacata actggcgcgt agtttactgg ttgcggcccc
    13861 gcttgacaaa aaactgcgta tcaaatgcag ataacgtaat aattgcctga gtggactatt
    13921 agaaagtcaa ggtgttcagg cgtttatttg taaagttttg ttgaaataag ggttgtaatt
    13981 gtgattacgc ccgcacataa cccacagggt gttgtctata ctttacacat aaggaagagg
    14041 ggtattccct gttacaaccc agaaagttcc ggaggtgaca tatgaaacgc aaaaacgctt
    14101 cgatactcgg taacgtgctc atggggttgg gtctggtggt aatggtggtc ggcgtggggt
    14161 attcaatcct caaccagtta ccacagttta atatgcccca gtatttcgca catggtgcag
    14221 tgctaagtat tttcgtcggt gccattctct ggctggcggg tgcccgtgtt ggcgggcatg
    14281 aacaggtgtg cgaccgttac tggtgggttc gtcactatga caaacgttgc cgccgtagcg
    14341 ataatcgccg tcatagctaa caaataatgc agtttgctga ccagtcagtt tgcgctgact
    14401 ggtcaatctc gtacttataa atccgccatc gccgcgcgac gattcgggaa gaacgcgagg
    14461 cgtcccggga tcggttgaat gcctgcgcga gccatagttc gcagtggctg gaattccacg
    14521 ttgcacacgc gcagttcaca tccttcgggc agacgcttca caaaacgctg gaacgcatca
    14581 agaccaccag catcaagtac cggaacggca tcccacttca gaatcacaat ccgtttgcct
    14641 tcaagacgtg actccaggtc cgtgaataag ccttcagcag cagcaaaaaa cagcgggcca
    14701 ataacgcgca gaaccaggac atcgtctgga acatctacga ctaccggtgc caggcgagtc
    14761 atacgtgcga tacgacgcat aaacagcagc gatgccagca cgatccccac gctgatggca
    14821 ataaccatat caaacagcac ggtcagtgac atacacagca gcatgacgat gatgtcatct
    14881 ttcggcgcat gacgcagcaa gtcgaccact ttgtgcgctt cactcatgtt ccacgccacc
    14941 atcaacagca gggctgccat ggcggaaagc ggcagccagg agagcagtgg tgccagtacc
    15001 agcagggcga gaataaccag aatagagtgg atcaccgccg agatagggga agttgccccg
    15061 gcacggacgt tagcggcaga acgcgcgatg gcagcagtag cggtaatacc accaaagaat
    15121 ggagcgatga tattccccag cccctgaccg accagttcac tattcgcctt gtgcttagtc
    15181 ccggtcatac catccagtac cacagcgcag agcagagatt cgatcgcccc gagcattgcc
    15241 attgagaatg ccgcaggcag cagggtgcga atagaatccc aggttagcgt gaattctgaa
    15301 ttaggcagat cccacggcag caccagttgc ggcagcagtt gcggaatacc gttaccctga
    15361 gaaccatcgg ccaggacgta gtggaattgc gaaccgatgg tagcaacatg tccgccgagc
    15421 aggttaacaa tccccatcac cgcgcaacca gccagcaagg ccggaaggtg accgggtaaa
    15481 cgaatgccca gacgcggcca gaaaaccaga atgccaagcg tcacaatgcc aatggcagca
    15541 tcacccacat taatggtcgg cagcgccata aataatgcgc cgactttttg tagataatgt
    15601 tccgggacat gggccatttg cagaccgaga aaatctttaa tctgcatggt accgatggtg
    15661 atcccgatac ccgaggtgaa acctaaggtg acggaaaccg gaatatactc aatcagacga
    15721 ccaaagcgtg ccagccccat cagaatcaaa aagattcccg acaacaaggt cgcaaccagc
    15781 agtcctgcca gtccaaactg ttgcgaaacg ggatagagaa ttaccacaaa tgccgcagtc
    15841 ggaccggaaa cgctaaagcg tgacccaccc gttagagcaa tgacaatccc cgcaacagct
    15901 gcggtatata aaccgtactg gggggccaca ccactaccaa tagccaacgc catcgccagc
    15961 gggatagcaa taatcccgac ggttatcccg gcaatcaggt cacgggtaaa ccgtgcggca
    16021 gtatattttt ctttccagca aacgtcgatc agagcgcgga aaggcatcac atgtgaggaa
    16081 aatattttgt tcacaataat gtttcatccg tgagcgcatc atctgtcaac taaatggcag
    16141 gtgaaggagg cataggtcat acaaatggat attacagaca aaaaaacccg ccgcagcggg
    16201 tctttgagcc gggttcgatt agtgttcgaa catggcagag atcgattctt cgttgctgat
    16261 acgacgaatc gcttcggcca gcatacctga cagggtcaga gtacgcacgt tcggcagtga
    16321 tttgatttca tcgctcagcg gaatggtatc gcagacaacg acttcatcaa ttacagagtt
    16381 acgcaggttg ttcgccgcgt tgccagagaa gatcgggtga gtcgcgtacg caaatacacg
    16441 tttagcacca cgttctttca gcgcttcagc agctttacac agcgtaccgc cagtgtcgat
    16501 catatcatcg accagtacgc agtcacgacc tgcaacgtca ccgatgatat gcatcacctg
    16561 ggaaacgttc gcacgcggac gacgtttgtc gatgattgcc atatcggtat cgttcagcag
    16621 cttagcgata gcgcgggcac gcacaacgcc gccgatgtcc ggagaaacca caattgggtt
    16681 atccagattc agctgcagca tgtcttccag caggatcggg ctaccaaata cgttatcaac
    16741 cggaacgtcg aagaaaccct gaatctgttc agcgtgcaga tccactgtca gcacacggtc
    16801 aacaccgacg ctggagagga agtctgcaac cactttcgca gtgattggta cacgagcgga
    16861 acggacgcga cggtcctggc gcgcatagcc aaagtagggg ataacagcgg tgatacgacc
    16921 tgcggaagca cgacgcaggg catcaaccat aacgactaat tccatcaggt tgtcgttagt
    16981 aggggcacaa gtggactgga tgatgaaaat atcaccaccg cgtacatttt cgttaatttg
    17041 tacgctgact tcgccgtcgc taaagcgacc tacagcggcg tcgccgagtg aagtgtacag
    17101 gcggttggca atacgttgtg ctagtaccgg ggtggcgtta ccagcaaaaa gcttcatatc
    17161 aggcacgaga agaacctcag gcatgcgtcc attggtggaa agaatctgcc gaaaactgtg
    17221 cgggccaggc atgatccttt ccaggcggtg tattaaagag cgcgatgcaa cgtctggaac
    17281 aaggtgacgt tgtcaccgaa actcagcttg cccggcttaa agcatggctc tgtgcaatgg
    17341 ggaaagatta acgcctttcg ccacaaagcc attgagccat tccggggctt gctctagcac
    17401 ctggcgggct tcagactctg tatcaaattc agcaaagaca caggcccctg tcccagtcag
    17461 gcgtgacggg gcgtattcta acagccagga aagcaccgca tcaacctcgc gaaaacgttt
    17521 tcttgcgata acctcgcaat cattgctgaa ttcacatttt agcaacgttt ctattgacct
    17581 ttttggcgta ttgcgcggga gttcaggatc tttaaaaatc accggagtcg gaatacttac
    17641 accagggtgc gccaccagat accacttctc tggcggatcc accggcgtta gtatttcacc
    17701 aacgccttcg gcaaacgcgg catgcccccg aacaaagaca ggaacatctg cgcccagcgt
    17761 cagccccatt tccgccagct catccatgct tagcccgcat tgccagagat gatttaatgc
    17821 caccaggacc gtcgcggcat tggatgaacc accgccgaga ccgccgccca tcggcaaacg
    17881 cttgtcaatg ctgatattcg caccgcttcc cgtcggaaga cgcccgctgt ctgccgcagt
    17941 tttcatcaac aaccgcgctg cgcgaacgat caggttatct tcatgttcca cgccttcaac
    18001 gggcgttaac agacgaatat ccccatcgtc acgaagctca atgctgatgg tgtcgccgta
    18061 atcaagaaac tgaaacagcg tttgcagcgt gtggtaacca tccgcacgct gaccggtaat
    18121 gtataaaaac agattaagtt ttgccggaga gggccactgt gtccgcatta tttcactatc
    18181 cagttatcca tttttaactt gatgcgttga ccaccgtcgg tgagttccat attggctggc
    18241 atcgcaggtt gcgttttggt gtcataacca ccataaacaa ccttccagtt tttgccattc
    18301 tggctgtagg taatttcgct caggcggtac tggtcgtcca gtttgtagtc ggttgcatca
    18361 cccggtaaac ctaaaatcca ctggcgcaag ctgttgagcg gaattggcat tccggtcaat
    18421 ttgccaatca tctcttcggc gtcatcggcg gtataacgct gacctttatt gtcgactaac
    18481 tgcacgttac ccggttgagc attcagctcc agttccgtgc tgcccaatgg gttagtgagc
    18541 agcagacggt agcgatcctg gccggtttgc tgccagaaaa agcgggcgta cactttttgt
    18601 tggtcagaaa tataagcgaa cgcgccgcga gtctgatact gattaagatt gcgcacgtct
    18661 tgctgatgct gacgccattg tggcgaatcc gggcttttgc caggaccttt gggcgtggta
    18721 acggaacagg cagtgagcac aagagcagcc agcggtagca ggcggataag acgaaaatcg
    18781 ggcaggggca tagtgatgac aagtccttga gatacgttgc agttataacc cttaatgcta
    18841 gcgttaccgt ccgctatcgt ctatgttcaa gttgtcttaa ttgccagaat ctaacggctt
    18901 tcggcaatta ctccaaaagg gggcgctctc ttttattgat cttacgcatc ctgtatgatg
    18961 caagcagact aaccctatca acgttggtat tatttcccgc agacatgacc cttttagcac
    19021 tcggtatcaa ccataaaacg gcacctgtat cgctgcgaga acgtgtatcg ttttcgccgg
    19081 ataagctcga tcaggcgctt gacagcctgc ttgcgcagcc gatggtgcag ggcggcgtgg
    19141 tgctgtcgac gtgcaaccgc acggaacttt atcttagcgt tgaagagcag gataacctgc
    19201 aagaggcgtt aatccgctgg ctttgcgatt atcacaatct taatgaagaa gatctgcgta
    19261 aaagcctcta ctggcatcag gataacgacg cggttagcca tttaatgcgt gttgccagcg
    19321 gcctggattc actggttctt ggggagccgc agatcctcgg tcaggttaaa aaagcgtttg
    19381 tcgattcgca aaaaggtcat atgaaggcca gcgaactgga acgcatgttc cagaaatctt
    19441 tctctgtagc gaaacgcgtt cgcactgaaa cagatatcgg tgccagtgct gtgtctgtcg
    19501 cttttgcggc ttgtacgctg gcgcggcaga tctttgaatc gctctctacg gtcacagtgt
    19561 tgctggtagg cgcgggcgaa accatcgagc tggtagcgcg tcatctgcgc gaacataaag
    19621 tacagaagat gattatcgcc aaccgcactc gcgaacgtgc ccaaattctg gcagatgaag
    19681 ttggcgcgga agtgattgcc ctgagtgaga tcgacgaacg tctgcgcgaa gccgatatca
    19741 tcatcagttc caccgccagc ccgttaccga ttatcgggaa aggcatggtg gagcgcgcat
    19801 taaaaagtcg tcgtaaccaa ccaatgctgt tggtggatat tgccgttccg cgcgatgttg
    19861 agccggaagt tggcaaactg gcgaatgctt atctttatag tgtggacgat ctgcaaagca
    19921 tcatttcgca caacctggcg cagcgtaaag ccgcagcggt tgaggcggaa actattgtcg
    19981 ctcaggaaac cagcgaattt atggcgtggc tgcgagcaca aagcgccagc gaaaccattc
    20041 gcgagtatcg cagccaggca gagcacgttc gcgatgagtt aaccgccaaa gcgttagcgg
    20101 cccttgagca gggcggcgac gcgcaagcca ttatgcagga tctggcatgg aaactgacta
    20161 accgcttgat ccatgcgcca acgaaatcac ttcaacaggc cgcccgtgac ggggataacg
    20221 aacgcctgaa tattctgcgc gacagcctcg ggctggagta gcagtacatc attttctttt
    20281 tttacagggt gcatttacgc ctatgaagcc ttctatcgtt gccaaactgg aagccctgca
    20341 tgaacgccat gaagaagttc aggcgttgct gggtgacgcg caaactatcg ccgaccagga
    20401 acgttttcgc gcattatcac gcgaatatgc gcagttaagt gatgtttcgc gctgttttac
    20461 cgactggcaa caggttcagg aagatatcga aaccgcacag atgatgctcg acgatcctga
    20521 aatgcgtgag atggcgcagg atgaactgcg cgaagctaaa gaaaaaagcg agcaactgga
    20581 acagcaatta caggttctat tactaccaaa agatcctgat gacgaacgta acgccttcct
    20641 cgaagtccgt gccggaaccg gcggcgacga agcagcgctg tttgcaggcg atctgttccg
    20701 tatgtacagc cgttacgccg aagcccgccg ctggcgggta gaaatcatga gcgccagcga
    20761 gggtgaacat ggtggttata aagagatcat cgccaaaatt agcggtgatg gtgtgtatgg
    20821 tcgtctgaaa tttgaatccg gcggtcatcg cgtgcaacgt gttcctgcta cggaatcgca
    20881 gggtcgtatt catacttctg cttgtaccgt tgcggtaatg ccagaactgc ctgacgcaga
    20941 actgccggac atcaacccag cagatttacg cattgatact ttccgctcgt caggggcggg
    21001 tggtcagcac gttaacacca ccgattcggc aattcgtatt actcacttgc cgaccgggat
    21061 tgttgttgaa tgtcaggacg aacgttcaca acataaaaac aaagcgaagg cgctttcagt
    21121 gctcggtgct cgcatccacg ctgctgaaat ggcaaaacgc caacaggccg aagcgtctac
    21181 ccgtcgtaac ctgctgggga gtggcgatcg cagcgaccgt aaccgtactt acaatttccc
    21241 gcaggggcgc gttaccgatc accgcatcaa cctgacgctc taccgcctgg atgaagtgat
    21301 ggaaggtaag ctggatatgc tgattgaacc gattatccag gaacatcagg ccgaccaact
    21361 ggcggcgttg tccgagcagg aataatggaa tatcaacact ggttacgtga agcaataagc
    21421 caacttcagg cgagcgaaag cccgcggcgt gatgctgaaa tcctgctgga gcatgttacc
    21481 ggcagagggc gtacttttat tctcgccttt ggtgaaacgc agctgactga cgaacaatgt
    21541 cagcaacttg atgcgctact gacacgtcgt cgcgatggtg aacccattgc tcatttaacc
    21601 ggggtgcgag aattctggtc gttgccgtta tttgtttcgc cagcgacctt aattccgcgc
    21661 ccggatacgg agtgtctggt ggagcaggca ctggcgcggt tgcctgaaca accttgccgt
    21721 attctcgatc tcgggacggg taccggggcg attgcgcttg cgctggctag cgagcgcccg
    21781 gactgcgaaa ttatcgctgt agatcgtatg cctgatgctg tctccctggc acaacgtaat
    21841 gcccagcatc tggcgatcaa aaatatccac attctgcaaa gcgactggtt tagcgcgcta
    21901 gccgggcagc agtttgcgat gattgtcagc aatccgccgt atattgacga gcaggaccca
    21961 catcttcaac aaggcgatgt ccgctttgag ccgctcactg cgctggttgc ggcagacagt
    22021 ggaatggcag acatcgtgca tatcatcgaa cagtcgcgta acgcgctggt atccggcggc
    22081 tttctgcttc tggaacatgg ctggcagcag ggcgaagcgg tgcgacaagc atttatcctc
    22141 gcggggtatc atgacgtcga aacctgccgt gactatggtg ataacgagcg cgtaacgctc
    22201 ggccgctatt atcaatgaca agtttttcta cactgcttag tgttcatctt attagtatcg
    22261 cgctttctgt tgggctatta accttacgtt tctggctacg ttatcagaag catccacagg
    22321 catttgctcg ctggacgcgc attgtgccgc cggttgtcga tacggtgtta ttgttaagcg
    22381 gcattgcgtt gatggctaaa gcgcacatcc tgccattttc cgggcaggca caatggctga
    22441 ctgaaaagct gtttggagtt atcatttata tcgttttggg ttttattgca ctcgattatc
    22501 gtcgtatgca cagtcagcag gcgcgcatta ttgccttccc gctggcgttg gtggtgctgt
    22561 acatcatcat taaactcgcc accacaaaag taccgttact ggggtaagtc atgagatcgt
    22621 tagctgattt cgaatttaat aaagcgccat tgtgcgaagg catgatcctg gcttgcgaag
    22681 caatccgccg cgattttccc tcgcaagatg tttacgacga actggagcgt ctcgttagtc
    22741 tggcgaagga agaaatcagc cagcttctgc ctttagaaga gcagctggaa aaactgatcg
    22801 cgctgtttta cggcgactgg ggatttaaag cctcacgcgg cgtttatcgt ctttccgatg
    22861 cattatggct ggaccaggtg ttaaagaatc gacagggcag tgcggtatca ttaggtgcgg
    22921 ttttattatg ggtcgcgaat cgtctcgatt tgccgctgct gccggtgatt ttccctacgc
    22981 agctgatatt gcgcattgaa tgtccggatg gcgaaatttg gctgattaat ccttttaacg
    23041 gtgaatcgtt aagcgaacat atgctggacg gatggttaaa gggaaatatc agcccgtcgg
    23101 cggaactgtt ttatgaagac cttgatgaag ctgataacat tgaggtaatc cgcaaattgc
    23161 tggatacact caaagcctcg ttgatggaag aaaatcagat ggagctggcg ttacgcacca
    23221 gcgaagcttt attacaattc aaccctgaag atccctatga aattcgcgat cgtgggttga
    23281 tttatgcgca actggattgc gaacacgttg cgttgaacga tttaagttat ttcgttgaac
    23341 ggtgtccgga agacccgatc agcgaaatga tccgtgcgca aataaataac atcgcgcata
    23401 aacatattgt gctgcattaa ttaatcgaca ttttactcaa gattaaggcg atcctatgaa
    23461 acaaaaagtg gttagcattg gcgacatcaa cgtagcaaat gacctgccgt tcgtactgtt
    23521 tggcggtatg aacgtgctgg aatctcgcga tctggcgatg cgcatttgcg agcactacgt
    23581 aactgtgacc cagaaactgg gtatccctta cgtgttcaaa gcctcttttg acaaagccaa
    23641 ccgctcctcc atccactctt atcgtggacc gggcctggaa gaagggatga aaatcttcca
    23701 ggagttgaag cagacttttg gcgtgaaaat tatcaccgac gttcacgaac caagtcaggc
    23761 acagcccgtt gctgatgtcg tggatgtgat tcagttgccg gcgtttcttg ctcgccagac
    23821 tgacctggtt gaagccatgg cgaaaaccgg tgcggtaatt aacgtcaaga aaccacagtt
    23881 tgtcagcccg ggacagatgg gtaatatcgt tgataaattc aaagaaggcg gcaacgaaaa
    23941 agtgattctt tgcgatcgtg gtgctaactt cggctatgac aacctggttg tcgatatgct
    24001 gggcttcagc attatgaaga aagtgtctgg taactcgccg gtgattttcg acgtgaccca
    24061 cgcactgcaa tgccgcgatc cgtttggcgc agcttccggt ggtcgtcgtg ctcaggtggc
    24121 tgagctggca cgagccggta tggcggtagg tctggcgggg ctgtttattg aagcgcatcc
    24181 ggatccggaa catgcgaaat gtgatggtcc atccgcgctg ccgctggcta aactggaacc
    24241 gttcctcaag cagatgaaag cgattgatga tctggtgaaa ggtttcgaag aactggatac
    24301 cagcaagtaa tcttttttgc ttgaaaaata aagtcttagc gttctgcgtt aagacttttt
    24361 tcatgggtgc cagatacaaa aaaggccgca ggctgttacc cctgcggccg gtttcgggcg
    24421 catattgcca tcacggcagc ctgacgcccg ttttcacctt acttccggtt acgccaccag
    24481 ctgacaatcg ctgcggcaat aatccccgcc aggatcggtg ctgccaggtc gtgccagaaa
    24541 gtcatggcaa actgcgcgag cgtcatatag ccgccttgtt gtaatgacaa cattttgcgg
    24601 ctattcttga agtgtctggt ttcaagatta gcccccgttt tgttgtcagg ttttacctct
    24661 caacgtgcgg gggttttctc tttccagcaa ccaatgccac cagggataaa gcccccgcaa
    24721 cattgcgcct caccggataa ttccggcttg gtgtggatac tacgtctcaa ttcatcttca
    24781 cttcatccct gaaatgtttg caataaagag tacattccgg cttttcaaca gctgttgcag
    24841 tggtttcgtg caggttgtgg tacaggctcg caattctgat taacgagctg tccaggtagt
    24901 atgaagaaat acaataagca ggaatttatc ttatctctcc cgatttccgt catcggagga
    24961 gatataactg tcaggcaaat atcgtcatca aataggcggc aaacagtgcc agatgcgctg
    25021 cgccattgag cacgttagta cgtccggtgg agaaggagat atggcacagc actaaagagg
    25081 ccaccatcac caccatttct ggcgcaccaa gtgcaaactg caattcgtta cccgtcataa
    25141 aggcaattag cgtgacgaca ggtacggtaa gcgaaattgt tgctaacact gaaccaaaga
    25201 acagattcat cgcgcgctga acctggttgt tcaacactgc ttttaacgca cctaaacctt
    25261 ccggcgacag aatcaacagt gccaccagga agccagtaaa ggcaacaggg gcattcatgc
    25321 tgtcgagcaa tgtctccagc gagctggcgt tcattttggt gactgcaata acggcaatca
    25381 gatggataat caaccagata gcatgccaca ggctgctatg ggcagacgtt ttaccgtggt
    25441 gcgggtcgtc gtcatcactg tcatcttcgt gctcgtagac aaacaaactt tgatgcgttt
    25501 tggcctggat cagcaaaaat acgccataca ttgccgcaga aattaatgct accagtaacg
    25561 cctgaccggt tgaaaaattc gccgcaggca gagccattgg aaataccagt acgattatcg
    25621 ccagggggaa cagcgcaatt aaatactgct tgataccaaa cagattcata tattgggtgg
    25681 caaacttacg accgcccaac aataatgaaa agccaaccag cccaccggta acaatcataa
    25741 tgattgaata gagcgtatca cgcattagcg ttggcgcggc gtcgccggtt gccattaaag
    25801 ctgaaatcaa actgacttca agaataacca ctgaaaggct aagaataagc gaaccgtaag
    25861 gttctcccag gcgatgggct aatacgtccg catgacggac aacactaaag gcgctactta
    25921 aaataccaat aagcgcaaga agattgatgg caatgaccac tggtagtgtc tggctacttc
    25981 cccacaggaa cagcactacc agcaccagaa ccgggaaaat aagcgaagtc tccttgtggc
    26041 gggtttttac cgcctcttga gcatttgaca ttatggttat ccctttgcag atgaatttat
    26101 cgaaaatgta aaaaataggt aggaaaaata acagaaagtg tctggatatc ggtaacattt
    26161 tacgaatttt taccccgctg tcgatttttt actcattggg gcataaaaat aagtattacg
    26221 tttagacaat gtttgtttta gcgtttctta agaacagtct gacatgaaaa ttcttatgtt
    26281 ttggcaagag tagatattgt tgaccacact taatgttcaa ctttgtaaaa ggagtcaacg
    26341 atgccgtata aaacgaaaag cgatctgccg gaaagcgtaa agcacgttct accgtctcat
    26401 gcccaggata tctataaaga agcgttcaac agcgcatggg atcaatataa agataaagaa
    26461 gatcggcgtg atgacgccag tcgcgaagaa accgcgcata aagtggcctg ggctgctgtg
    26521 aagcatgaat atgccaaagg ggatgatgat aaatggcata aaaaatcgta aaaccggtag
    26581 cttagttaaa gctattcgtg cggtgttgcc ttgcaagtgg tccgtggatt gcatattgtt
    26641 ccgttagtgg tttcaaaatg agcggtaaaa atgtccggaa gacaccaaaa agttgtcgca
    26701 gggaagtatg cagtggcgga ggtgtaaggt gataacgcgt gatttcttga tgaatgccga
    26761 ttgtaaaacg gcatttggtg ccattgaaga atcactctta tggtcagcag aacaacgggc
    26821 ggcttcgctg gcggcgacgc tggcttgtcg acctgatgag ggaccggtgt ggatcttcgg
    26881 ttatggatcg ttgatgtgga atccggcact ggagtttacc gaatcatgca ccggtacact
    26941 ggttggatgg catcgcgcgt tttgcctgcg cctgaccgcc gggcgcggga ctgcgcacca
    27001 gccgggacgg atgcttgcac tgaaagaggg cggacgcacc acaggcgtcg cctatcgact
    27061 accagaagag acgctggagc aggaactaac cctgttgtgg aagcgcgaga tgattaccgg
    27121 ctgttatctg ccaacctggt gtcagcttga tcttgatgat ggacgcacag taaacgccat
    27181 tgtgtttatt atggacccgc gacatccaga atatgaatct gacactcgcg ctcaggtcat
    27241 cgcgccgttg attgcggcgg cgagcggtcc gctgggaacc aatgcacaat acctgttttc
    27301 actggaacag gagcttatca aactgggaat gcaggacgat gggctgaatg atttgctggt
    27361 atcggtaaaa aaactactgg cggagaatta tccggatggt gtgttaaggc cgggattcgc
    27421 ctgagtaaac ttccctcata gtggggcgtc agacgccccc ttaaacatta aaatgtgagc
    27481 actttatcgg ctgacagcgt ccattgcgcc agttccacaa gagtaccgat ttccacccca
    27541 tcaatcagag gaagtgtgct aatcccgcgc ccgtcggtac aggttttgca caatttcacc
    27601 ggtacattct gagcggtaag gatctccagc atttgctgaa tgttgtagcc ttcccctggt
    27661 ttttgcccgc gcaacccggc tgtgaccgca tcagacatca ggaacagacg cagatccaga
    27721 ttgctctcct gctctcgtaa cgcaatggcc agccgcaagc tgttaaacaa ggattcgctc
    27781 ccgtaaggtg cgccattggc aacgatcact attttttgca ttatttactc ctgtattcag
    27841 ggaattagac actcatcttc tatcttactg cttctgcagc gtctgaccaa tcggtcacat
    27901 ttttaaggat tttcctgaaa gcgcgagaaa atacgacaaa agttgccagt aatcgttatt
    27961 ctttaaggct atggtttttc attattaccg gaagttaccg acgttttgag ccgtttcgtt
    28021 cctcgcatta tcccgtttta tttactcttg cttgtagcag gcggtacagc taacgcacaa
    28081 tctaccttcg agcaaaaagc ggcaaatccc tttgataata acaatgatgg tctgccggat
    28141 ttaggcatgg ctcccgaaaa tcatgatggg gaaaaacact ttgctgaaat tgtgaaagat
    28201 ttcggcgaaa ccagtatgaa tgataacggg ctggatactg gcgagcaggc aaaagctttc
    28261 gcattgggaa aagtccgcga cgcgcttagt caacaggtta atcagcacgt agagtcctgg
    28321 ctatcaccgt ggggaaatgc cagtgttgac gtcaaagtgg ataacgaagg acatttcacc
    28381 ggcagtcgtg gaagctggtt tgtgccgtta caagataatg atcgttatct cacctggagc
    28441 cagcttggtc ttactcagca ggataatggg ttggtgagca atgtgggcgt tgggcaacgc
    28501 tgggcgcgcg gcaactggct ggtgggttat aacacttttt atgacaactt gcaggacgaa
    28561 aatcttcagc gagcgggctt tggtgccgaa gcgtggggcg aatatttgcg attatcggca
    28621 aacttttatc agccgtttgc tgcatggcat gaacagacag ccacgcagga acaacggatg
    28681 gcgcgcgggt acgacctgac agctcggatg cgcatgccgt tctatcaaca cctcaatacc
    28741 agtgtcagcc tagaacagta ttttggtgat cgtgttgatt tgtttaactc tggtacgggt
    28801 tatcacaatc ccgtcgcgtt gagtctggga ttaaattaca cccctgtgcc attagtcact
    28861 gtgacggccc agcataaaca gggggaaagt ggcgaaaatc aaaataacct cgggctgaat
    28921 cttaattacc gctttggtgt accgctcaaa aaacaacttt ctgcgggcga ggttgccgaa
    28981 agtcagtcgt tacgtggtag tcgctatgac aatccgcagc gaaataatct accgactctt
    29041 gagtaccgac agcgaaaaac gttaacggtg tttctggcga caccgccgtg ggatctaaaa
    29101 cctggcgaaa cagtgccgct gaaattacaa atccgcagtc gttacggtat tcggcaactg
    29161 atttggcagg gcgatacgca gatattaagt ttgacgccgg gcgcacaagc caacagcgcg
    29221 gagggctgga cgctgatcat gcctgactgg cagaacgggg aaggggcaag caatcactgg
    29281 cgattgtctg tggtggtgga agataaccag gggcagcgtg tctcctccaa tgagatcacg
    29341 ctaacgcttg tcgaaccgtt cgatgcattg tcaaacgacg aactgcgctg ggaaccgtaa
    29401 tcagaaaatg cgctcctgat gcacccatac cgctgcttcc acgcgagact tgagcttcat
    29461 tttcttcagc atgtgcttga cgtgcacttt tactgtgctt tcggtgatat ccaggcggcg
    29521 ggcaatcatc ttgttcggca aaccctgggc aatcagcttg agaatatcgc gctcgcgtgg
    29581 ggttaactgg ttaacatcgc gctcagtagt ggcacggtta gcgcgcaagc tggcggccag
    29641 aacaggcgtt aatgcttcgc ttaataccat ttcgccagca gctgcctgat gcaatgcttt
    29701 cagcagatct tccggttcca tatcttttaa cagatagcca tccgcgccgc gtttcagtgc
    29761 ggtgaccaca tcttcttcat ggttagagac gctgaatacc acaatgcgcc ctgagaggga
    29821 cttttcgcgc agtttatcca gcgtttccag accgttcatg ccgggcatat tgagatctaa
    29881 caggatcaga tcgggatcaa gagattccgc cagttcaata ccctgttcgc cattactcgc
    29941 ttcgccaacc acggtgatat ctggtgccat actgataagc tgttttacgc cagttcgcag
    30001 catcgggtga tcgtcaatca gcaggatagt agccggttcc tgattactca tgggtatctc
    30061 cttggacgtc tgtgaaagtt ttttcgggaa taaaggtgac caccacttcg gtgccacctg
    30121 attcacgacg gcggacgcgg caatcgcctc gtaaactttg cgcacggtcg cgcattatta
    30181 tcatgccgta gtgattgctg cggatggcat tttcaggcac gccacagccg ttatcctgga
    30241 cggtcagttt gacctgatta tcgttttgcg ccaccgtcac cacgacctca ctcgcttgcg
    30301 aatgtttgag ggcgttactt aatgcctcac gggcaatttg caacaagtgg attgcctgat
    30361 gtgaaggcac cagacgaggc ggcaattgat aatccagctt caccgggaag ccaaatttgg
    30421 cgctgtactc ttcgcaactc gcttccagcg ccggacgtaa tccaggctcg gtgagctgca
    30481 agcggaatgt ggtgagcaat tcacgcaact gcgcccagga tgcattcagt tcgttacgga
    30541 tctgacttaa cagttcgcgg ctgctttctg gcagcgcatc gccctgcatc tgtaaacaac
    30601 tcacctgcat cttcatgcaa gagagagatt gggcaataga atcatgcagt tcgcgcgcaa
    30661 tggtggcacg ctcttccatc acgatcaact gttgctgacg ttcctgatgg cgatccagcg
    30721 ccagcgtggc ggtaagttgt tcaaccaggg tatccaccag ttgttgttgg tcatggctaa
    30781 gatgacgccc ctgcggcagg gtcgccagca aaataccgta ctgcgtatga gagtcagcca
    30841 gccgccactt cagggtcgtg ccgcgatcac caacgggtaa tacgccgcgc gggcagagct
    30901 ggcagccttt atcatcacaa gtcatatctg gctggcaggt aaactcctga tgattctctt
    30961 catcatcagt gtcatacacc cgcagttcga tatcacgtag cagggttaaa ttctgtaagc
    31021 cgttgagtac cggtgacagg cgttcacaca gcggggcgcg ggaatgcaaa cggcggttag
    31081 cctgccataa aaaagagagg atctgatttt tatgctccag cccggcggtt ttctcctgaa
    31141 cccgctgctc aagtacggca taactttcgg ccagttctgc agacatattg ttcaacgcag
    31201 ttccaagcat cgccatttcg ttgcgcccgc tgatgtttgc gcgttgggta aaatcgcgat
    31261 ggctgacggc actcgccatt gccagcagtt gccgccacgg ttgtagcagt cgcgcccgca
    31321 accagataat agtgaacacc agtaaaagtg ccataaatac cgccattacc cgatggacca
    31381 gtaccactgt ctcaatacgc atttccgtgg tgcggtcaaa accagatacc agctgatcaa
    31441 gcccggcaac aaactggctg acatccgctg acaccgtttc tcggttttgt gcacgcatca
    31501 gcgcagggat cagttcatta cgccagtaat cttgtaagcc ctgtaattgc gccagttgtc
    31561 cgtctcgttc tgctgctcga gtcaactcgg cgctaaatgc cgtttgttcc atctctttaa
    31621 ttaagggctt gtctttctcg cttaatggca ctgccgccaa cagacggtaa ctttgcatgc
    31681 gcagcgatcc cgctttgttg atcgcatggg cgctgccctg aacgccttgc accagccagc
    31741 cagaaaccgc catccctgcc agtccaatag cagtagaaag caacacaata agcgcaacct
    31801 gattaaccag ggtgagcgga gagagacaac gtttaagcat gtaaacccct tccttcaggc
    31861 tttaaatgag caataacctt aatgaatgtg acgatatatt ctggaatggc agtattctcg
    31921 gctattggct gaagtatacc catagccgga aagagttact ccttatttgc cgtgtggtta
    31981 gtcgctttac atcggtaagg gtagggattc tacagcaccg tgaaaaatct cataattttt
    32041 atgaaatcac tgtactcact atgggtaatg ataaatatca atgatagata aagttatctt
    32101 atcgtttgat ttacatcaaa ttgcctttag ctccagacac taaggtggca gacatcgaaa
    32161 cgagtatcag aggtgtctat gagtcactca tctgcctccg aaagggctac tggagctgtc
    32221 ataacagatt ggcgaccgga agatcctgcg ttctggcaac aacgcggtca acgtattgcc
    32281 agccgcaacc tgtggatttc cgttccctgt ctgctgctgg cgttttgcgt atggatgttg
    32341 ttcagcgctg ttgcggtgaa cttaccgaaa gtcggtttta attttacgac cgatcagcta
    32401 tttatgttga ctgcgctgcc ttcggtttct ggcgcgttat tacgtgttcc atactccttt
    32461 atggttccta tcttcggtgg tcgtcgctgg acggcgttca gcaccggtat tctgattatt
    32521 ccttgcgtct ggctgggttt tgccgtgcag gatacctcca cgccttatag cgtcttcatc
    32581 atcatctctc tgctatgcgg ctttgctggc gcgaacttcg catccagtat ggcaaacatc
    32641 agcttcttct ttccgaaaca gaagcagggt ggcgcgctgg gtctgaatgg tggtctggga
    32701 aacatgggcg tcagcgtcat gcagttggtt gctccgctgg tggtatcact gtcgattttc
    32761 gcagtatttg gtagccaggg cgtcaaacag ccggatggga ctgagctgta tctggcgaat
    32821 gcgtcctgga tatgggtgcc gttccttgcc atcttcacca ttgcggcgtg gtttggcatg
    32881 aacgatcttg ctacctcgaa agcctccatc aaggagcagt tgccggtact caaacggggt
    32941 catctgtgga ttatgagcct gctgtatctg gcaaccttcg gctccttcat cggcttctcc
    33001 gcgggctttg cgatgctgtc aaaaacgcag ttcccggatg ttcagattct gcaatacgct
    33061 ttcttcgggc cgtttattgg tgcgctggcg cgttctgcag gtggtgcatt atctgaccgt
    33121 ctgggcggaa ctcgtgtcac gctggtgaac tttattctga tggcgatttt cagcggcctg
    33181 ctgttcctga ccttaccgac tgacgggcag ggcggaagct tcatggcgtt cttcgcggtc
    33241 ttcctggcgc tgttcctgac agctgggctg ggtagtggtt ccactttcca gatgatttca
    33301 gtgatcttcc gtaaactgac aatggatcgc gtgaaagcag aagggggttc tgacgaacgt
    33361 gcgatgcgtg aagcggcaac cggcacggca gcggcgctgg gtttcatctc tgcgattggc
    33421 gcgattggtg gcttctttat cccgaaagcg tttggtagct cgctggcatt aacgggttcg
    33481 ccagtaggtg caatgaaagt atttttgatt ttctatatcg cctgcgtagt gattacttgg
    33541 gcggtatatg gtcggcattc taaaaaataa atcttaatat ttgattatcc tttgcgcggc
    33601 atgatgccgc gcttttttta tgcgtcattt agttacaaca tattagtgtt atatggctta
    33661 tttcgccgga tttcattaag agccattaat atgttaccca tggggaatac tccttaatac
    33721 ccatttgcat aaaaatc
//
LOCUS       DAEBXN010000037        31187 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.037, whole genome shotgun sequence.
ACCESSION   DAEBXN010000037 DAEBXN010000000
VERSION     DAEBXN010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31187
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.037"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            389..2047
                     /locus_tag="KJL99_001805"
     CDS             389..2047
                     /locus_tag="KJL99_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HBD4436555.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(2044..3000)
                     /locus_tag="KJL99_001806"
     CDS             complement(2044..3000)
                     /locus_tag="KJL99_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HBD4436556.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRCSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3151..4266
                     /gene="macA"
                     /locus_tag="KJL99_001807"
     CDS             3151..4266
                     /gene="macA"
                     /locus_tag="KJL99_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HBD4436557.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4263..6209
                     /gene="macB"
                     /locus_tag="KJL99_001808"
     CDS             4263..6209
                     /gene="macB"
                     /locus_tag="KJL99_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HBD4436558.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLNIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDIATLDADALAQLRREHFGFIFQRYHLLPHLT
                     VEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6282..6506)
                     /gene="cspD"
                     /locus_tag="KJL99_001809"
     CDS             complement(6282..6506)
                     /gene="cspD"
                     /locus_tag="KJL99_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HBD4436559.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6829..7149
                     /gene="clpS"
                     /locus_tag="KJL99_001810"
     CDS             6829..7149
                     /gene="clpS"
                     /locus_tag="KJL99_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HBD4436560.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            7180..9456
                     /gene="clpA"
                     /locus_tag="KJL99_001811"
     CDS             7180..9456
                     /gene="clpA"
                     /locus_tag="KJL99_001811"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HBD4436561.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     KLSDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9859..9946)
                     /locus_tag="KJL99_001812"
     tRNA            complement(9859..9946)
                     /locus_tag="KJL99_001812"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9910..9912),aa:Ser,seq:gga)
     gene            complement(10200..10418)
                     /gene="infA"
                     /locus_tag="KJL99_001813"
     CDS             complement(10200..10418)
                     /gene="infA"
                     /locus_tag="KJL99_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HBD4436562.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10703..11407)
                     /gene="aat"
                     /locus_tag="KJL99_001814"
     CDS             complement(10703..11407)
                     /gene="aat"
                     /locus_tag="KJL99_001814"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HBD4436563.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPDDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(11449..13170)
                     /gene="cydC"
                     /locus_tag="KJL99_001815"
     CDS             complement(11449..13170)
                     /gene="cydC"
                     /locus_tag="KJL99_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HBD4436564.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSVMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARTLLHDAPLVLLDEPTEGLDAT
                     TESQILELLAKMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(13171..14937)
                     /gene="cydD"
                     /locus_tag="KJL99_001816"
     CDS             complement(13171..14937)
                     /gene="cydD"
                     /locus_tag="KJL99_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HBD4436565.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAAKLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(15060..16025)
                     /gene="trxB"
                     /locus_tag="KJL99_001817"
     CDS             complement(15060..16025)
                     /gene="trxB"
                     /locus_tag="KJL99_001817"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBD4436566.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMARMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16569..17063
                     /gene="lrp"
                     /locus_tag="KJL99_001818"
     CDS             16569..17063
                     /gene="lrp"
                     /locus_tag="KJL99_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HBD4436567.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            17198..21226
                     /gene="ftsK"
                     /locus_tag="KJL99_001819"
     CDS             17198..21226
                     /gene="ftsK"
                     /locus_tag="KJL99_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBD4436568.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNSAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAA
                     EAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVAPQQQYQQPQQQVAPQPQYQQPQQPVAPQQQYQQPQQPV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQDTLLHPLLMRNGDSRPLHKPTTPLPSL
                     DLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGV
                     KAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRD
                     NPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDV
                     RFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNL
                     AGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKK
                     VEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILD
                     QAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSD
                     SESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEA
                     QGIVSEQGHNGNREVLAPPPFD"
     gene            21381..21992
                     /gene="lolA"
                     /locus_tag="KJL99_001820"
     CDS             21381..21992
                     /gene="lolA"
                     /locus_tag="KJL99_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HBD4436569.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            22003..23346
                     /gene="rarA"
                     /locus_tag="KJL99_001821"
                     /gene_synonym="mgsA"
     CDS             22003..23346
                     /gene="rarA"
                     /locus_tag="KJL99_001821"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HBD4436570.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDDTRRAIAELVNGDARRALNTLEMM
                     ADMAEVYDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            23437..24729
                     /gene="serS"
                     /locus_tag="KJL99_001822"
     CDS             23437..24729
                     /gene="serS"
                     /locus_tag="KJL99_001822"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBD4436571.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            24969..27412
                     /gene="dmsA"
                     /locus_tag="KJL99_001823"
                     /pseudo
     CDS             24969..27412
                     /gene="dmsA"
                     /locus_tag="KJL99_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            27423..28040
                     /gene="dmsB"
                     /locus_tag="KJL99_001824"
     CDS             27423..28040
                     /gene="dmsB"
                     /locus_tag="KJL99_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HBD4436572.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            28042..28833
                     /locus_tag="KJL99_001825"
     CDS             28042..28833
                     /locus_tag="KJL99_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HBD4436573.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGWAMRLL
                     PAISVLALVVSGVMSVMQGTELATIHSSVQQAAALVPDYGALMSWRIVLLAVALCLWI
                     APQLKGYQPAVPLLSVSFILLLAGELIGRGVFYGLHMTVGMAVAS"
     gene            complement(28869..29495)
                     /locus_tag="KJL99_001826"
     CDS             complement(28869..29495)
                     /locus_tag="KJL99_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBD4436574.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVLELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            29810..30958
                     /locus_tag="KJL99_001827"
     CDS             29810..30958
                     /locus_tag="KJL99_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD4436575.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLSSFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFSAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
BASE COUNT         7690 a         7644 c         8374 g         7479 t
ORIGIN      
        1 tggtcgtttt atagggtcgt cgtaaaaaat gacgacggaa ataatgcgcg gctattttaa
       61 aaacgaaggc gagtcattca ccagataaat aaatccagta aatttgattt agggcaacag
      121 cgggttgccc catatagtca tttgtctgat tgacagtgta gtgcaagcaa aagatttaat
      181 cctttaggcg taataaaaaa taatttatca tgctaattat ttgattttgt tgtttttgcg
      241 ggcttatcag caagagggag tataacgcga ttattcgctc atttttcaga caattgccat
      301 gcttaaatgt gatgtcatca cgtattagca aggcctttcc cgttatactg cgagcgtaaa
      361 ggataagtca catatttctg gaggggatat gattcttgag cgcgttgaaa ttgtgggttt
      421 tcgcggtatc aaccgtttgt cgttgatgct cgaacaaaac aacgtcctga ttggggagaa
      481 cgcgtggggt aaatccagct tgctggacgc cttaaccctg ctgctatcgc cagaatcaga
      541 tctctaccat tttgagcgcg acgatttctg gttcccgccg ggagatatca acgggcgaga
      601 acatcatctg catattattt tgaccttccg cgaatcgctg ccaggccgac atcgggttcg
      661 ccgttatcgg ccgctggaag cgtgctggac gccatgcacc gatggctatc accgtatttt
      721 ttatcgtctg gaaggggaga gtgcggaaga cggcagcgtg atgacactgc gcagttttct
      781 cgataaagac ggacatccga ttgatgttga ggatattaac gatcaggcac gccatctggt
      841 gcgtttaatg ccggtgctgc gcttgcgtga tgcccgtttt atgcgccgta ttcgtaacgg
      901 cacggtgcca aatgtcccca atgtggaagt caccgcgcgc cagctcgatt tcctcgcccg
      961 tgagttgtcc tcacatccgc aaaatctctc tgatggacag attcgtcagg gactttccgc
     1021 aatggtgcag ttgcttgagc attatttctc tgagcagggg gctggacagg cgcgatatcg
     1081 tttaatgcgg cggcgggcca gcaatgagca acgaagctgg cgctatctgg acatcatcaa
     1141 ccggatgatt gaccgacctg gagggcgctc gtatcgggtt attttgctcg gcctgtttgc
     1201 tactttattg caggcaaaag gcacattgcg actggataaa gacgcgcgtc cgttattgct
     1261 aattgaagat ccagaaaccc gcctgcatcc gattatgctt tccgttgcct ggcatctgtt
     1321 gaatcttttg cctttacagc gtattgctac caccaactct ggcgagctgc tttcgttaac
     1381 tccagtggaa catgtttgcc gcctggtgcg tgaatcttct cgtgttgccg cctggcgtct
     1441 ggggccaagt ggcttgagca ccgaagacag ccgccgcatc tcttttcaca ttcgttttaa
     1501 tcgtccgtca tcgctgtttg ctcgctgctg gttgctggtg gaaggggaaa cggaaacctg
     1561 ggttatcaat gaactggcgc gtcagtgcgg acatcatttt gatgccgaag ggatcaaggt
     1621 cattgagttt gcccagtccg ggctaaagcc actggttaaa tttgcccgcc gaatggggat
     1681 tgaatggcat gtactggtcg atggcgatga agcagggaag aaatatgccg ctacggtacg
     1741 cagcctgttg aataacgatc gggaagccga acgagaacat ttaacggcgt taccggcgct
     1801 ggatatggaa cattttatgt atcgccaggg attttccgat gtgttccacc gcgtggcgca
     1861 aatcccggaa aatgtaccga tgaatctacg caaaattatc tcgaaagcga tccatcgctc
     1921 ttccaaaccc gatcttgcca ttgaagtggc aatggaggca ggacgtcgtg gtgtggactc
     1981 cgtaccaacg ctgctgaaaa aaatgttctc acgcgtgctg tggctggcgc gcggtcgcgc
     2041 ggattaaccg cgaaacatcg tggccatttg tggctgaata gcgtcgagca tctcatagcg
     2101 ccgacggtat tcagcccgtt ttttactggc gatttcggca atctcttttc gtgctatctg
     2161 tgctggaagg cggtaatggc gttcagcatc acatacgccg ccaaccgact cccagaacgc
     2221 gttgtaatca gcatggatct tgccttcttt atcgcgataa cgcaggctgc ggtaaatatg
     2281 cgtttcattg ctgacggcaa taatctgctc tacctgcaaa cgttgggcaa acagacaggc
     2341 cgcttccatc acgaggcgtt tgggaaatag cccgtggcag gctttcgtcg cattctggat
     2401 ttcctgatgt ggaatttccc attttgcgcc ttgcagtccg ccaataaaca tcgttctttt
     2461 cccctgatat tcacacaggg taaacgtgat ctctgccaga ggaatacctt cgctgttgcg
     2521 gaacaggatt gtgctgtcac cttctttatc cattgagatc atcatggtca gctcaagcgt
     2581 gaactgctca ccgtttttgc cttccagttt agccagttgc agcccggggg tattcaaata
     2641 taagctgaat tcttccgccg acatacaccc gcggagtaac gcataatggt aacgtaacgc
     2701 ctccagcaat tgcttacggc taagattcgc ggcaaggtaa gggcgatgca gacgcacagg
     2761 cagtcgcggc tggcgcgtta acaacacatt gagattaggc cagtgggaaa gttcgttcat
     2821 ccactcaacg cttaaacgcg gcataatcaa cgagcgcagc aaaaatttct ggcgaaaact
     2881 acagcgatgc cagaatttac ccggccgaca ctgtccacgt gccagactaa gaaaaagtga
     2941 caggctgctg agagattcag atggcgtaaa ggtccgttca gttagctgcg acatattcat
     3001 gaaatcaatg gttatacatg acgtcgattt caccattgcg tatcttaacc aaacatcaat
     3061 agtgtgatta ctaacgtaaa ttttagggtt ttgttgatat ttcgttgaag ttaatgaccc
     3121 ggattggcat atggagtatt cagaatattt atgaaaaagc ggaaaaccgt gaagaagcgt
     3181 tacgttattg cgctggtgat agtcatcgcc ggactgatta cgttatggag aattcttaac
     3241 gcacccgtgc cgacttatca gacactgatt gtgcgccccg gtgatttaca gcaaagcgtg
     3301 ctggcgaccg gaaagctgga cgcgctgcgt aaggttgacg tgggcgcgca ggtcagcggt
     3361 cagttgaaaa cgctgtcggt ggcgattggc gataaagtaa aaaaagacca gcttttaggg
     3421 gtcattgatc ctgaacaggc tgaaaaccag atcaaggagg tcgaagcaac gctgatggag
     3481 ctacgcgcgc agcggcagca ggcggaagcg gagctgaaac tggcgcgggt gacgtattcc
     3541 cgtcagcaac gtctggcaca aacgcaagcg gtttcactgc aggatctcga taccgccgcg
     3601 acggagatgg ctgtgaaaca ggcgcaaatt ggcaccattg acgcgcaaat caagcgcaat
     3661 caggcctctc tcgatacggc taaaaccaat ctcgattaca cccgtatcgt tgccccgatg
     3721 gctggggaag tgacgcaaat caccactctg caaggccaga cggtgattgc cgcacagcaa
     3781 gcaccgaaca ttctgacgct ggcagatatg agcactatgc tggtaaaagc gcaggtttct
     3841 gaagcggatg taatccacct gaagccgggg caaaaagcct ggtttacggt gcttggcgat
     3901 ccactgacgc gctacgaggg gcaaatcaag gatgtgctac cgacgccgga aaaggtaaac
     3961 gacgctattt tctattacgc ccgttttgaa gtacccaacc ccaatggttt gctgcggctg
     4021 gatatgaccg cgcaagtgca tattcagctc accgatgtga aaaatgtgtt gacgatccct
     4081 ctgtcggcgt taggcgatcc ggttggcgat aatcgttata aagtcaaatt gttgcgtaat
     4141 ggtgaaacac gcgagcgtga agtgacgatt ggcgcacgta acgataccga tgttgagatt
     4201 gtcaaagggc tggaagcggg cgatgaagtg gtgattggtg aggccaaacc aggagctgca
     4261 caatgacgcc tttgctcgaa ttaaaggata ttcgtcgcag ctatcctgcc ggtgatgagc
     4321 aggttgaggt gctgaagggc atcagcctca atatttatgc gggtgagatg gtcgcgattg
     4381 ttggcgcttc gggttccggt aaatcgaccc tgatgaatat tctcggctgc ctggataagg
     4441 ccaccagcgg cacctatcgc gtagccggtc aggatattgc cacgctggac gccgatgcgc
     4501 tggcgcaact gcgccgcgag catttcggct ttattttcca gcgttaccat ttgcttccgc
     4561 atttaaccgt cgagcagaac gttgaagtac ccgccgtcta tgccggtctt gagcggaaac
     4621 agcgactgct tcgtgcccag gagttgctgc aacggctggg gctggaagac cgtacagagt
     4681 attatccggc acagctttcg ggtggtcagc aacagcgcgt cagcatcgcg cgggcattga
     4741 tgaacggcgg tcaggtaatt cttgccgatg aaccgaccgg cgcactggac agccattctg
     4801 gcgaagaggt gatggcgatc ctgcatcagc tgcgcgatcg cgggcatacg gtgattatcg
     4861 tcacccacga tccgcaggtc gctgctcagg ccgagcgggt gatcgaaatt cgcgacggcg
     4921 aaattgtgcg caatcctccc gccattgaaa aagtgaatgt tgctggcggg acggagcctg
     4981 ttgtcaatac agtgtctggc tggcggcagt ttgtcagcgg ttttaacgag gcgctgacga
     5041 tggcatggcg ggcgctggca gcgaataaaa tgcgtacttt actgaccatg ctggggatta
     5101 ttatcggtat tgcgtcggtg gtttccattg tcgtggtggg tgacgccgcc aaacaaatgg
     5161 tgctggcgga tattcgttct attggtacga atactattga tgtctatccc gggaaagatt
     5221 ttggcgatga cgatccgcaa tatcagcagg cgctgaagta cgacgactta atcgccatcc
     5281 aaaaacaacc gtgggtcgcc tcagccacac ctgccgtctc gcaaaacctg cgcctgcgtt
     5341 ataacaatgt tgatgttgct gccagtgcca atggcgtgag cggcgattat tttaatgtct
     5401 atggcatgac cttcagtgaa ggaaacacct ttaatcagga gcagctgaac ggtcgtgcgc
     5461 aggtcgtggt tctcgacagt aatactcgcc gccagctttt cccccataaa gcagatgtgg
     5521 ttggcgaggt gattctggtc ggcaatatgc ccgccagagt cattggtgtg gcggaagaaa
     5581 aacagtcgat gtttggtagc agtaaagtgc tgcgtgtctg gctaccttac agcacgatgt
     5641 ccgggcgagt tatgggccag tcgtggctta actccattac tgtcagggtg aaagaaggat
     5701 ttgacagcgc cgaggcggaa cagcaactca cgcgtttact ttcactgcgc cacggtaaga
     5761 aggatttctt tacctggaac atggacggcg tcttgaaaac tgttgaaaag accacacgta
     5821 ctttacaact gtttctgacg ctggtggcgg tgatttcgct ggtggtgggc ggtattggtg
     5881 taatgaatat tatgctggtg tcagtgaccg agcggacgcg ggaaattggc attcgcatgg
     5941 ctgtaggtgc gcgagcaagc gatgttttgc aacagttcct gatcgaagcc gtactggttt
     6001 gcctggtcgg tggcgcgttg ggaataacac tgtcactgtt aattgctttc accttgcagc
     6061 ttttcttacc cggctgggag attggttttt caccgttggc gctgctgctg gcgtttctct
     6121 gctcgacggc caccgggatt ttatttggct ggttacccgc acgaaatgcg gcacgactgg
     6181 atccagtaga tgctctggca cgggagtaat ttttgagata aaaatgccag ccgatcgggc
     6241 tggcattttg cctttaggat gtacacaatg agacagaaga gctatgcgac tgccgcttct
     6301 acttcgacgg gcacaataac actggcgtga ttgccttttg gcccctggtg gacatcaaac
     6361 tgaacggatt gtccagcttt tagcgttctg taaccatcca tctgaatggt ggaataatga
     6421 gcgaaaatat cttcgccgcc gccttcaggg cagatgaaac caaacccttt ggcattgttg
     6481 aaccacttaa cagtaccctt ttccatgctt cgacatcctt cgcaaatctt atacaagtaa
     6541 gatggaataa accggggtca gagagggggc tgttcaaaac ctcgccaact ctagaaatac
     6601 aatttagaga attagggcga gccgtcaagc atttgacagg ggccaggggg caggtatgaa
     6661 tcaaaaattt gaagcagtta acgctattga caggaatgtg acagatgtcg ctgatgccaa
     6721 cgatagatga tagttatcta tcatgtggag tagattggtc aggcaaataa gctcttgtca
     6781 gcggcagggc gttctgccga taaccgtaac cgaagatgat aactgacaat gggtaaaacg
     6841 aacgactggc tggactttga tcaactggcg gaagaaaaag ttcgcgacgc gctaaaaccg
     6901 ccatctatgt ataaagtgat attagtcaat gatgattaca ctccgatgga gtttgttatt
     6961 gacgtgttac aaaaattctt ttcttatgat gtagaacgtg caacgcaatt gatgctcgct
     7021 gttcactacc aggggaaggc catttgcgga gtctttaccg ccgaggttgc agaaaccaaa
     7081 gtggcgatgg tgaacaagta cgcgagggag aatgagcatc cattgctgtg tacgctagaa
     7141 aaagcctgaa tgcaggcata aaaattgggg gaggtgccta tgctcaatca agaactggaa
     7201 ctcagtttaa atatggcttt cgccagagcg cgcgagcacc gtcatgagtt tatgaccgtc
     7261 gagcacttgt tactggcgct gctcagtaac ccatctgccc gggaggcgct ggaagcgtgt
     7321 tctgtggatt tggttgcgct ccgtcaggaa ctggaagcct ttattgaaca aaccacaccc
     7381 gttctgcctg ccagtgaaga ggagcgcgac acacagccga cgctgagttt tcagcgtgta
     7441 ctgcaacgtg cggtcttcca tgtccagtcc tccggtcgca atgaggttac cggtgcaaac
     7501 gttctggtcg ctatcttcag cgaacaggag tcgcaggcgg catatctgtt gcgtaaacat
     7561 gaagtcagcc gtctcgatgt ggtgaacttt atctctcatg gcacgcgtaa agacgagccg
     7621 acacagtctt ctgatcctgg cagccagcca aacagcgaag aacaagctgg tggggaggaa
     7681 cgtatggaga atttcacgac gaacctgaac cagcttgcgc gtgtgggcgg aatcgaccca
     7741 ctaattggtc gtgagaaaga gctggagcgt gctattcagg ttctctgccg tcgccgtaaa
     7801 aacaacccgc tgctggtggg ggaatctggt gtcggtaaaa ccgcgattgc agaaggtctt
     7861 gcctggcgaa ttgttcaggg tgatgtgccg gaagtgatgg ctgactgtac gatttactct
     7921 ctcgatatcg gttctctgtt agcgggcaca aaatatcgcg gcgactttga aaaacgtttt
     7981 aaagcgttgc tcaagcagct ggagcaggac actaacagca tcctgtttat tgatgagatc
     8041 cacaccatta tcggtgcggg tgcagcgtct ggtggtcagg tcgatgcggc taacctaatc
     8101 aaaccgttgc tctccagcgg taaaattcgc gtaattggtt cgacaaccta tcaggagttc
     8161 agcaacattt tcgagaaaga ccgtgctctg gcgcgtcgct tccagaaaat tgatattact
     8221 gaaccgtcga tcgaagaaac tgttcaaatc atcaatggcc tgaaaccgaa gtatgaagcg
     8281 caccacgacg tgcgttatac cgcaaaagcg gtgcgtgcgg cggtagagct ggcggtgaaa
     8341 tacattaacg atcgtcatct gccggataaa gccattgatg ttatcgacga agcaggcgct
     8401 cgcgcacgtc tgatgccggt aagcaaacgc aagaaaaccg ttaatgtggc ggatattgag
     8461 tccgtggtgg cccgtattgc acgcattcca gagaagagtg tttctcagag tgaccgcgat
     8521 accctgaaaa aactcagcga tcgcctgaaa atgctggtct tcggccagga taaagccatt
     8581 gaggcgctga ctgaagccat taagatggcg cgtgcaggtt taggtcacga acataaaccg
     8641 gtcggttcgt tcctgtttgc cggtcctacc ggggtcggga aaacagaggt gacggtacag
     8701 ctttcgaaag cgttgggcat tgagcttctg cgctttgata tgtccgagta tatggaacgc
     8761 cataccgtca gccgtctgat tggtgcgcct ccgggatacg ttggttttga tcagggcggt
     8821 ttgctgaccg atgcggtaat caagcatcca catgcggtgc tgctgctgga cgaaatcgag
     8881 aaagcgcatc cggacgtgtt caatattctg ttgcaggtga tggacaacgg tacgctgacc
     8941 gataacaacg gacgcaaagc ggacttccgt aacgtggtgc tggtgatgac cactaacgcc
     9001 ggggtacggg aaactgagcg caaatccatt ggtcttatcc accaggataa cagcaccgat
     9061 gcgatggaag agatcaagaa gatctttaca ccggaattcc gtaaccgtct cgacaacatt
     9121 atctggttcg atcatctgtc aactgacgtg atccatcagg tggtggataa attcatcgtc
     9181 gagttgcagg ttcagctgga tcagaaaggt gtttctctgg aagtgagcca ggaagcgcgt
     9241 aactggctgg ccgagaaagg ttacgaccgg gcaatgggcg cacgtccgat ggcgcgtgtc
     9301 atccaggaca acctgaaaaa accgctcgcc aacgaactgc tgtttggttc gctggtggac
     9361 ggcggtcagg tgacggttgc gctggataaa gagaaaaatg agctgactta cggattccag
     9421 agtgcacaaa agcacaaggc ggaagcagcg cattaatctg attgtcaggt aggttggtga
     9481 agtccgtaat ctcgtcaggg gttacggact ttttatttat ggggtggaac ggtgaaaacc
     9541 ctatttttgg aggtgaaggt aagttgttga taattagtgc tgctggaagg taaggataaa
     9601 aaagggtgct gcaggagaat gggatggttt tgctttatta acaacgggct aaacgtgtag
     9661 tatttgagtt cactgccgta caggcagctt agaaatcgac ggggtgcggt aaaacctttg
     9721 cgaacgcgtt cactgccgta caggcagctt agaaattcac aggtaacata ctccacccgc
     9781 ccaccatgtt cactgccgta cagacagata aaatgcgaaa aaaaagctcg cactttcgta
     9841 cgagctcttc tttaaatatg gcggtgaggg ggggattcga acccccgata cgttgccgta
     9901 tacacacttt ccaggcgtgc tccttcagcc actcggacac ctcaccaaat tgttttgcta
     9961 ccaaacctca tgggtggcaa cggggcgcta ctatagggag ttggagtaaa acggtcaaga
    10021 agaattttaa tgataattat tgtttgctca tactgtaaac aacttgtgca gtatatctac
    10081 atcgagacaa gttatggact tatacttcca aagtacttca tacatatcac aaaataaaaa
    10141 ggccggttaa accgaccttt tactcgttct ttctcttcgc ccatcaggcg gtaaaacaat
    10201 cagcgactac ggaagacaat gcggcctttg ctcaggtcgt acggggtcag ttcaacagtc
    10261 actttgtcgc ccgtcaggat gcggatgtag tttttgcgca ttttaccgga gatgtgtgca
    10321 gtaaccacgt gaccgttttc taactctacg cggaacatgg tattaggcaa cgtttcaaga
    10381 acggtacctt gcatttcaat attgtcttct ttggccatct aatcctctgg ggtatcacta
    10441 ccgtaatttg aaccggcaag ataatgccga agttctgtaa ataagtaaag atttgcgcgc
    10501 taaatcgcaa caaacaggtt cggcacatta ctccgaaaac acacggctaa gccgcaccaa
    10561 aagcgcaacg tataagggag cggtgagata aacgatgggc gttacctgac gcgaaaaatt
    10621 ccttatcggc agcggggtaa tgagcgtaac caactctgcg accgcaatta taacactctg
    10681 gggagaaatg tgccgaaaac attcattctt gtggtgaaaa caagcaccgt ggtacccaga
    10741 aattattcgg caatcgtccg aggcgcattt gattgagata attaaggtaa tcccggcggg
    10801 gaatttcgca ggcaccaagc gatgctgtgt gatcgttaag gacctggcag tcgataagct
    10861 taccgccatg accgataaat tcctcacaga ataccagaag cgccgtttta gacgcatttt
    10921 ccatccggct gaacatggac tcgccacaaa atagcgttcc ctgggccacg ccgtacatac
    10981 cgccgacaag ctcatcttca cgccagactt caatggagtg ggcatgaccg agttcgtgaa
    11041 ggcgatggta ggcttcgacc acgccacgcg tgatccaggt tccttcttcg cgatcgctgg
    11101 cacagccttc aatgacctga ccaaaagcgt aattcatcgt gacacgatag ggcgagcgtt
    11161 tatgaaatcg cttcatacta cggctgatat gcagtgattc tggccatagc accgcgcggg
    11221 gatcgggcga ccaccagagg atggggtcgt ctggagaaaa ccacggaaaa ataccacgct
    11281 ggtaagccat taacaggcgc gcagggctaa gatcgccccc aagtgccagc aggccgttag
    11341 gctcacgtaa tgcgccttcc ggggaaggga aggctattga atggcgagaa agctgaacca
    11401 agcgcatgac cgcaaaactc cacgcaagtc ggatcgttca ataatagctt acaaaccctg
    11461 cttgaactgg taataacgcc cctgtctggc aagcagttct gcgtgagtac cttgctcaat
    11521 aatttgcccg ttgtccatca ctattatttg ttggaaacga gagagtccgc gaagtcgatg
    11581 ggtgaccatt aacaccgttt tctcacgcat catttttgca agcaattcaa ggatctggct
    11641 ttcggttgtg gcatctaagc cttcggtagg ttcatccagc aacaccagtg gcgcatcatg
    11701 taacagcgta cgggcgatag ccagacggcg cagttcacca ccggagagct ggcgtccgcc
    11761 ttcacctaac caactgttga gacctgcatc ctcgagcagc ttctccagac caacgcgacg
    11821 caagatctcc gacagagcct catcactact gccaggcgag gcgagtaaca gattatcacg
    11881 cagtgtggcg ctaaacagat gcactcgctg aggaacaacg ctgatggtct gtcgtagagc
    11941 cgcttcattc aggctggcta tggggctatc gttaagcaaa atctcgccct gttgcgggtc
    12001 ccatgcgcgg gtcagcagtt gtaacagtgt tgatttgccg catccggttc gcccgagaat
    12061 cgctatatgt tccccggcgt ttacctgaag agaaatccct ttaagtgcct gttgggattg
    12121 ctccggataa gtgaactgaa catcccgtaa tgtcagcgaa acgcgatcgg caacacgagt
    12181 ttgggtatca gggaaggtga cctccggttt ttgatccgtt aagtcagaga tacgtacggc
    12241 agaggcaatg acttgcccca gatgctgaaa tgcacccgtt actggtgcca gtgcttcaaa
    12301 cgcggctaac gcacaaaaga caaacagggc aattaatgcg ccgggttgag cattgccgcc
    12361 aacgccgcca gacgccatcc acagcatcag gatcaccgct aacgcgccaa tgagcagcat
    12421 tatcgcttgc gacaatgcgg tcagttcaga ttgacggcgt tgcgcttcca gccattgaat
    12481 ttctgtattc tctagttgcg tgcgataacg atcgctggca ccaaaaatgg tcagctcagc
    12541 ttgcccttgc agccaggccg tcagttgttg gcgatactgt ccgcgaagat gagtcagatt
    12601 ttgcccggtg ctttttcccg cacgataaaa cagcggtggc atcaggaaaa gcgtcagtaa
    12661 cataacgctg cccagcgtaa aggcgagggt gaaatcaagg aaacttaacc cgattgtcac
    12721 caccataatc accacaaaag cgcccaccag cggcgagata acgcgcaggt aaagatgatc
    12781 gagcgtatca acatccgcca ccacgcggtt gagcaattcg ccctgacgat agcgcgccag
    12841 tccggcaggg gagaggggca acaatttgct gaaggtgtaa atgcgcagat gctgcaacac
    12901 gcggaaagtc gcgtcgtgac ttaccagacg ttcaaaataa cgcccggcag tacgggtgat
    12961 tgcggcacca cgcacgcccg cagcgggtag catatagttg aagctgtaca gtccggcaac
    13021 ccccgcaacc gctgaggccg agaggaacca gccggaaagt gtcaacagac cgatactggc
    13081 gagcagcgtc acaattgcca gcacaatacc aagacttaac atccatttat gacgtttata
    13141 cagtgccaga tagggtagca aagcgcgcat ttaaatctcc tcctgacgat gggccagtaa
    13201 tgtggcgaat gggccaccag ccacacttaa ttccgcgtaa cgtccttgct caataatccg
    13261 gccatcctgc ataacccaaa tgacatccca gtcagcaaga tcttctaact ggtgggtgac
    13321 cattaacgtt gtctggcgca gagaggcggc attcagcgcc tccattacgc gctgttcact
    13381 gtgagcatca aggctggcag cgggttcatc caacagtaat agcgaacagg gatttagtaa
    13441 cgcacgggcc accgccacgc gctgcgcctg ccccacggaa aggcgggcag cctggtcgcc
    13501 aacaggtgta tcaacgcctt gtggcaggag cggtagaaac tcgctgaccc aggcgttatc
    13561 cagcgctgct tgtaattctt gttcgctggc atcaggtcgc gccagtagta cgttatcccg
    13621 caattttgct gccggtaatt gtgggttttg cccaacccag gagagatgtt tacgccatga
    13681 ttctggtgat aaatcacgta attctatccc gttgattcgt aatgatccct gatatgagag
    13741 aaaaccagaa agcgcgttca gcagtgagct tttacctgaa ccgctgcggc caaccaacac
    13801 tgcacgttgg cctgctggca aagtaaagtt cagcggtccg gccagcgttt taccttccgg
    13861 cgacgtgata aacagatcct cggcctcaat ggtcagcgga tcggtcaatg ctaattccgc
    13921 ctcaccgcgc tgcggatggg cgagcggggt ttccataaac gttttcagac tgtcagctgc
    13981 accaacagcc tgggctttag catgataaaa cgtaccgaga tcgcgtaatg gctggaaaaa
    14041 ctctggcgca aggatcaggg ccagaaaacc tgcagccagc gtcaccccag tatcgtagtg
    14101 accaaaatcc agctcgccga gataggaaaa accaaagtag accgccacca gagcaattga
    14161 cagcgaggta aaaaattcga gaatgccgga ggataaaaac gccagtcgta gcacttccat
    14221 tgtccgttgg cggaaatctt ccgaagcaga acgaatactt tcaatttcag cttcaccacg
    14281 accaaaaata cgcaatgttt ccatgccgcg caggcgatcg aggaaatgcc cacttaagcg
    14341 agcaagagcg agaaagttac gccggttagc atcggcagcc cccattccaa ccagcgccat
    14401 aaacaacgga attagcggtg ctgtgcccag cagaatgagc gccgcagccc agttagaggg
    14461 gaagatcgcc accacaatca gcaacggcac cgacactgcc agcgccattt gcggcagata
    14521 gcgtgcatag taatcatgca tatcgtcaat ttgctcgagt accagcgtcg cccagctccc
    14581 cgcaggttta ccctgaatcc acgctggccc tgcttgttgc agacggtcga gaacctgacg
    14641 gcggatggca aagcggatat gctgcccggc gtgataaccc acccgttcgc gtaaccagac
    14701 cacccatgcg cgcaatacaa aggtcagaac cagtaacgta aagggaagca gcagggcttc
    14761 acggggaata ttctccataa tcatatgttg cagaattcgc gccatgaacc aggcctgggc
    14821 aatgatcaat atgccgctta caaagcccag cagacgagaa atattcagcc aacgttggga
    14881 gatgacgctt tgctgtttta accagcgggt taactctttt tgacgagatt tattcattgc
    14941 acgcttagca ggtgagttat cagaattatt tgcagagcaa tgttacaacg gggaaaaaat
    15001 aaaggcgacc catagtcgca aggtgtcgcc ttctttactt ttgttactga tttgtaaaat
    15061 tattttgcgt cagctaaacc atcgaggtag cgttccgcat caagtgctgc catgcagcca
    15121 gtaccggccg aagtaatggc ctggcgataa atgtgatcca tcacgtcgcc tgcggcaaag
    15181 acgccaggaa tgctggtctg ggtggcatta ccatgaatac ccgactgtac tttgatgtag
    15241 ccgttttcca gttccagctg cccttcgaaa atcgcagtat tcgggctgtg accgatagca
    15301 acaaacagac cggcaacgtc gagtgactcg atgttatcgc tgttttgcgt atcgcgcaga
    15361 cgaacgccag tgacacccat ttgatcgccg gtcacttctt ccagcgtacg gttggtgtgc
    15421 agaatgatgt tgccgttctc cactttatcc atcagacgct taataaggat tttttccgcg
    15481 cggaaaccgt cacggcggtg aatcagatgc acttccgaag cgatgttaga cagatacagc
    15541 gcctcttcaa ccgcggtatt gccgccgccg atgaccgcaa ctttctggtt gcgatagaag
    15601 aaaccgtcgc aggttgcaca agcagaaacc ccacggcctt taaaggcttc ttcagagggc
    15661 aggccgagat agcgtgcaga agctccggtg gcaataatca gcgcgtcgca agtgtattcg
    15721 ccgttatcgc cattcagacg gaacggacgg ttttgcagat ccaccttgtt gatatgatca
    15781 aaaatgatct cagtttcaaa cttggtggca tgttcgtgca tgcgcgccat taataacgga
    15841 ccggtcagat cgtttggatc accaggccag ttttccactt ccgtggtggt ggtcagttgg
    15901 ccgccttttt ccatgccggt aatcagcaca ggttgcaggt tggcgcgcgc cgcgtagaca
    15961 gcagcggtgt atcccgccgg gcctgaaccc aggataagca gtttactgtg tttggtcgtg
    16021 cccatgagat ccccatagtt gttggcagac aatgggcagg attgtaggga atttacagac
    16081 gtaaaaaaag agtatgacga ttttgttaac aatttgtgca atcggcagca tcgataagca
    16141 ggtcaaattc tcccgtcatt atcacctctg ctacttaaat ttcccgcttt ataagccgat
    16201 taaatgatga ataaacgccc ctgttaatga atatctggca tgttgtacta aaaatcgatg
    16261 ttttgctttg acaatcccct ggtgttttgc gaaaacattc gaggaagaaa aaaacagtat
    16321 tcttatatgc gcataaccat gtatgtaaat accatgttta ccgtgctagt gaaatctacg
    16381 tatggcgtgg acagacgcca ttcgtgatgt cgatagctgc cacaaggcaa cggtcttctc
    16441 accgtagacc caggcattgc gcgccgtgaa tcttcatgat ttcggtctat cgtgacgggt
    16501 agcgactctg aacagtgatg tttcagggtc agacaggagt agggaaggaa tacagagaga
    16561 caataataat ggtagatagc aagaagcgcc ctggcaaaga tctcgaccgt atcgatcgta
    16621 acattcttaa tgagttgcaa aaggatgggc gtatttctaa cgtcgagctt tctaaacgtg
    16681 tgggactttc cccaacgccg tgccttgagc gtgtgcgtcg gctggaaaga caagggttta
    16741 ttcagggcta tacggcgctg cttaaccccc attatctgga tgcatcactt ctggtattcg
    16801 ttgagattac tctgaatcgt ggcgcaccgg atgtgtttga acaattcaat accgctgtac
    16861 aaaaacttga agaaattcag gagtgtcatt tagtatctgg tgatttcgac tacctgttga
    16921 aaacacgcgt gccggatatg tcagcttacc gtaagttgct gggggaaacc ctgctgcgtc
    16981 tgcctggcgt caatgacaca cggacatacg ttgttatgga agaagtcaag cagagtaatc
    17041 gtctggttat taagacgcgc taacacggaa caggtgcaaa atcggcgtat tttgattaca
    17101 ctcctgttaa tccatacagc aacagtactg gggtaacctg gtactgttgt ccgttttagc
    17161 atcgggcagg aaaagcctgt aacctggaga gcctttcttg agccaggaat acactgaaga
    17221 caaagaagtc acattgacaa agttaagtag cggccgccgc cttctggagg cattgctgat
    17281 ccttattgtc ctgtttgccg tctggttgat ggctgcctta ctaagcttta acccttcgga
    17341 ccccagctgg tcgcaaacgg cctggcatga acctatccat aatttaggtg ggatgcccgg
    17401 tgcgtggttg gcagatacgc tgttctttat ttttggcgtg atggcttaca ccattcccgt
    17461 cattattgtc ggcggttgtt ggtttgcctg gcgtcatcag tccagcgacg aatacattga
    17521 ttattttgcc gtttcgctac gcatcattgg cgttttggcg ctcatcctta cctcctgtgg
    17581 tctggcggca atcaacgctg acgatatctg gtattttgcc tccggtggcg tcattggcag
    17641 cttactaagc actacgctac aaccactgct acacagtagc gggggaacta ttgcgctgct
    17701 ctgcgtttgg gcagcgggcc tgacgttgtt caccggttgg tcatgggtga ccattgctga
    17761 aaaactcggc ggctggattt taaacattct caccttcgcc agtaatcgta cccgtcgcga
    17821 tgatacctgg gtcgatgaag atgagtatga agacgacgaa gagtatgaag atgaaaatca
    17881 cggcaaacag catgaatcac gccgtgcccg tattcttcgc ggcgcgctag cgcgtcgtaa
    17941 acggttggcg gaaaaattca ttaatccgat ggggcggcaa acagacgctg cgttgttctc
    18001 cggtaagcgg atggatgatg acgaagagat tacctacact gcacgcggtg tggctgccga
    18061 cccggacgac gtcctatttt cgggcaatcg tgcaacgcag ccagaatatg acgaatacga
    18121 tccattatta aacagtgcgc caattaccga acctgtcgct gtagcagctg ctgctaccac
    18181 ggcgacacaa agctgggctg cgccggttga acctgtgact cagacgccgc ctgttgcctc
    18241 tgttgatgtt ccaccttcgc aacctacagt agcctggcag cctgtaccgg gtccacaaac
    18301 gggagagccg gttattgctc ctgcaccgga aggttaccca cagcagtcac aatatgcgca
    18361 gcctgcagtg caatataatg agccgctgca acaaccagta cagccgcagc agccgtatta
    18421 tgcacctgca gctgaacaac ctgcgcaaca gccgtattat gcccctgcgc cagaacaacc
    18481 ggtggcaggt aacgcctggc aagccgaaga gcagcaatcc acttttgctc cacagtctac
    18541 ataccagact gagcaaactt atcagcagcc agccgctcag gagccgttgt accaacagcc
    18601 gcaacccgtt gaacagcagc ctgttgtgga gcctgaaccc gttgtagaag agacaaaacc
    18661 cgcgcgtccg ccgctttact attttgaaga agtggaagag aagcgagccc gtgaacgtga
    18721 acaacttgcg gcctggtatc aaccgattcc agaaccggtt aaagaaccag aaccgatcaa
    18781 atcttcgctg aaagcacctt ctgttgcagc agtacctcca gtagaagccg ctgccgctgt
    18841 ttctccgctg gcatctggcg tgaaaaaagc gacactggcg acgggggctg ccgcaaccgt
    18901 tgccgcgcca gtcttcagtc tggcaaatag cggtggaccg cgtccgcagg tcaaagaggg
    18961 gattggcccg cagttgccac gaccgaaacg tatccgcgtg ccaactcgtc gtgaattggc
    19021 gtcttacggt attaagctgc cctcacagcg tgcggcggaa gaaaaagccc gtgaagccca
    19081 gcgcaatcag tatgattctg gcgatcagta taacgatgat gaaatcgatg cgatgcagca
    19141 ggatgaactg gcacgtcagt tcgcccagac acagcagcaa cgctatggcg aacagtatca
    19201 acatgatgtg cccgtaaacg cagaagatgc agatgctgcg gcagaggctg aactggctcg
    19261 tcagtttgcg caaactcaac aacaacgtta ttccggcgaa caaccggctg gggcgaatcc
    19321 gttctcgctg gatgattttg aattttcgcc aatgaaagcg ttgctggatg atggtccaca
    19381 cgaaccgctg tttacgccaa ttgttgaacc tgtacagcag ccgcaacaac cggttgcacc
    19441 acagcagcag tatcagcagc cacaacagcc ggttgcgcca cagcagcagt atcagcagcc
    19501 acaacagcag gttgcgccgc agccacaata tcagcagccg caacaaccgg ttgcgccaca
    19561 gcagcaatat cagcagccgc aacaaccagt tgcgccgcag ccgcagtatc aacagccgca
    19621 acaaccggtt gcaccgcagc cgcagtatca gcagccacaa cagccagttg cgccacagcc
    19681 gcaggatacc ctgcttcatc cgctgttgat gcgtaatggc gacagccgtc cgttgcacaa
    19741 accgacgacg ccactgcctt ctctggattt gctgacaccg ccgccgagcg aagtggagcc
    19801 ggtagatacc tttgcgcttg aacaaatggc gcgtctggtg gaagcgcgtc tggctgattt
    19861 ccgtattaaa gccgatgtcg ttaattactc tccggggcca gttatcactc gctttgaact
    19921 taacctggct cctggcgtaa aagcggcgcg catttctaac ttgtcacggg accttgcccg
    19981 ttcactttcg acggtggcgg tgcgtgtcgt tgaagttatt cctggcaaac cctatgtagg
    20041 tctggagtta ccaaataaaa aacgacaaac cgtttatctg cgcgaagttt tggataacgc
    20101 caaattccgc gataatccgt cgccattaac cgtggtgctg ggtaaagata tcgccggtga
    20161 gccggtggtt gccgatctgg cgaaaatgcc gcacttgttg gttgcgggga ctaccggttc
    20221 cggtaaatct gtcggtgtga acgcgatgat cctgagcatg ctttataaag cacagccaga
    20281 agatgtgcgt ttcatcatga tcgacccgaa aatgctggag ctttcggttt atgaaggcat
    20341 tccgcacctg ttaacggaag tcgttactga tatgaaagat gccgccaacg cgctgcgctg
    20401 gtgtgttaac gagatggagc gtcggtataa actgatgtct gcgctgggtg tgcgtaatct
    20461 ggcgggttat aacgaaaaaa ttgctgaagc cgatcgcatg atgcgtccga ttccagaccc
    20521 gtactggaag ccgggtgaca gtatggatgc ccagcatccg gtgctgaaaa aagaaccata
    20581 cattgtcgtg ttggttgacg aatttgccga cctgatgatg acggtaggta aaaaagtgga
    20641 agagctgata gctcgtctgg cgcaaaaagc ccgtgccgcg ggtatccacc tcgtactggc
    20701 aactcagcgt ccatcggttg atgttattac tggtctgatt aaagcgaata ttccgacccg
    20761 tatcgccttt accgtatcca gtaagattga ctcacgtacc attcttgatc aggctggcgc
    20821 ggaatcactg ctgggtatgg gggatatgct ctactctggg ccgaactcca cgttgccggt
    20881 acgtgtccat ggtgcttttg ttcgcgatca ggaagttcat gccgtggtgc aggactggaa
    20941 agcgcgtggt cgcccacagt atgttgatgg catcacctcc gacagcgaaa gcgaaggtgg
    21001 tgcgggtggt ttcgatggcg ctgaagaact ggatccgttg ttcgatcagg cggtgcagtt
    21061 tgtcactgaa aaacgcaaag cgtcaatttc tggcgtacag cgtcagttcc gcattggtta
    21121 taaccgtgca gcgcgtatta tcgaacagat ggaagcgcag gggattgtca gcgaacaggg
    21181 gcacaacggt aatcgtgaag tgctggcccc accgccgttt gactaattaa tgcattgccg
    21241 gataaggcgc ggtagcgtcg catccggcac tctatcaact gaaaattcag tattttcttc
    21301 tttcctcaag ctgattatta gcctggaata gagagtagag ggaactcccg atcgggagtg
    21361 acgtaatttg aggaataatg atgaaaaaaa ttgctatcac ctgtgcatta ctctcaagct
    21421 tagtagcaag cagcgtttgg gctgatgccg caagcgatct gaaaagccgc ctggataaag
    21481 tcagcagctt ccacgccagc ttcacacaaa aagtgactga cggtagcggc gcggcggtgc
    21541 aggaaggtca gggcgatctg tgggtgaaac gtccaaactt attcaactgg catatgacac
    21601 aacctgatga aagcattctg gtttctgacg gtaaaacact gtggttctat aacccgttcg
    21661 ttgagcaagc tacggcaacc tggctgaaag atgccaccgg taatacgccg tttatgctga
    21721 ttgcccgcaa ccagtccagc gactggcagc agtacaatat caaacagaat ggcgatgact
    21781 ttgtcctgac gccgaaagcc agcaatggca atctgaagca gttcaccatt aacgtgggac
    21841 gtgatggcac aatccatcag tttagcgcgg tggagcagga cgatcagcgc agcagttatc
    21901 agctgaaatc ccagcaaaat ggggctgtgg atgcagcgaa atttaccttc accccgccgc
    21961 aaggcgtcac ggtagatgat caacgtaagt agaggcacct gagtgagcaa tctgtcgctc
    22021 gatttttcgg ataatacttt tcaacctctg gccgcgcgta tgcggccaga aaatttagca
    22081 cagtatatcg gtcagcaaca tttgctggct gcggggaagc cgttgccgcg cgctatcgaa
    22141 gccgggcatt tgcattctat gatcctctgg gggccaccgg gtaccggcaa aacaaccctc
    22201 gctgaagtga ttgcccgcta tgcgaacgct gatgtggaac gtatttctgc cgtcacctct
    22261 ggcgtgaaag agattcgcga ggcgatcgag cgcgcccggc aaaaccgcaa tgcaggtcgc
    22321 cgcactattc tttttgttga cgaagttcac cgtttcaaca aaagccagca ggatgcattt
    22381 ctgccacata ttgaagacgg caccatcact tttattggcg caaccactga aaacccgtcg
    22441 tttgagctta attcggcact gctttcccgt gcccgtgttt atctgttgaa atccctgagt
    22501 acagaggata ttgagcaagt actaactcag gcgatggaag acaaaacccg tggctatggt
    22561 ggtcaggata ttgttctgcc agatgataca cgacgtgcca ttgcagaact ggtgaatggc
    22621 gacgcgcgcc gggcgttaaa tacgctggaa atgatggcgg atatggccga agtctatgat
    22681 agcggtaagc gggtcctgaa gcctgaatta ctgaccgaaa tcgccggtga acgtagcgcc
    22741 cgctttgata acaaaggcga tcgcttttac gatctaattt ccgcactgca taagtcggta
    22801 cgtggtagcg cacccgatgc ggcgctgtac tggtatgcgc gaattattac cgctggtggc
    22861 gatccgttat atgtcgcgcg tcgctgtctg gcgattgcgt ctgaagacgt cggtaatgcc
    22921 gatccacggg cgatgcaggt ggcaattgcg gcctgggatt gctttactcg cgttggcccg
    22981 gcggaaggtg aacgcgccat tgctcaggcg attgtttacc tggcctgcgc gccaaaaagc
    23041 aacgctgtct acactgcgtt taaagccgcg ctggccgatg ctcgcgaacg cccggattat
    23101 gacgtgccgg ttcatttgcg taatgcgccg acgaaattaa tgaaggaaat gggctacggg
    23161 caggaatatc gttatgctca tgatgaagca aacgcttatg ctgccggtga ggtttacttc
    23221 ccgccggaaa tagcacaaac acgctattat ttcccgacaa acaggggcct tgaaggcaag
    23281 attggtgaaa agctcgcctg gctggctgaa caggatcaaa atagccccat aaaacgctac
    23341 cgttaatgtt atcgttgcgg taatgttgtt actgtatccc tgtggtcgca ggctgtggcc
    23401 acatctccca tttaattcga taagcacagg ataagcatgc tcgatcccaa tctgctgcgt
    23461 aatgagccag acgcagtcgc tgaaaaactg gcacgccggg gctttaagct ggatgtagat
    23521 aagctgggcg ctcttgaaga gcgtcgtaaa gtattgcagg tcaaaacgga aaacctgcaa
    23581 gcggagcgta actcccgatc gaaatccatt ggccaggcga aagcgcgcgg ggaagatatc
    23641 gagcctttac gtctggaagt gaacaaactg ggcgaagagc tggatgcagc aaaagccgag
    23701 ctggatgctt tacaggctga aattcgcgat atcgcgctga ccatccctaa cctgcctgca
    23761 gatgaagtgc cggtaggtaa agacgaaaat gacaacgttg aagtcagccg ctggggtacc
    23821 ccgcgtgagt ttgactttga agttcgtgac cacgtgacgc tgggtgaaat gcactctggc
    23881 ctcgactttg cagctgcagt taagctgact ggttcccgct ttgtggtaat gaaagggcag
    23941 attgctcgca tgcaccgcgc actgtcgcag tttatgctgg atctgcatac cgaacagcat
    24001 ggctacagtg agaactatgt tccgtacctg gttaaccagg acacgctgta cggtacgggt
    24061 cagctgccga aatttgctgg cgatctgttc catactcgtc cgctggaaga agaagcagac
    24121 accagtaact atgcgctgat cccaacggca gaagttccgc tgactaacct ggtgcgcggt
    24181 gaaatcatcg atgaagatga tctgccaatt aagatgaccg cccacacccc atgcttccgt
    24241 tctgaagccg gttcatatgg tcgtgacacc cgtggtctga tccgtatgca ccagttcgac
    24301 aaagttgaaa tggtgcagat cgtgcgccca gaagactcaa tggcggcgct ggaagagatg
    24361 actggtcatg cagaaaaagt cctgcagttg ctgggcctgc cgtaccgtaa aatcatcctt
    24421 tgcactggcg acatgggctt tggcgcttgc aaaacttacg acctggaagt atggatcccg
    24481 gcacagaaca cctaccgtga gatctcttcc tgctccaacg tttgggattt ccaggcacgt
    24541 cgtatgcagg cacgttgccg cagcaagtcg gacaagaaaa cccgtctggt tcataccctg
    24601 aacggttctg gtctggctgt tggtcgtacg ctggttgcag taatggaaaa ctatcagcag
    24661 gctgatggtc gtattgaagt accagaagtt ctgcgtccgt atatgaacgg actggaatat
    24721 attggctaat acccaatttt tctgaatcta aaaagcgcct ttagggcgct ttttttgtct
    24781 ccctttgata ccgaacaata attactcctc acttacacgt aatactactt tcgagtgaaa
    24841 atctacctat ctctttgatt ttcaaattat tctatgtata caagcctata tagcgagctg
    24901 ctatagaaat aattacacaa tacggtttgt tactggaatc aatcgtgatc aagcttgagt
    24961 gagccattat gaaaacgaaa atccctgatg cggtattggc tgctgaggag agtcgccgtg
    25021 gtttggtaaa aacgacagcg atcggcggcc tggcaatggc cagcagcgca ttaacattac
    25081 cttttagtcg gattgcgcac gctgtcgata gcgccattcc aacaaaatca gacgaaaagg
    25141 ttatctggag cgcctgtaca gttaactgtg gtagtcgctg cccgctacgt atgcacgtcg
    25201 tggacggtga aatcaaatat gtcgaaacga caataccggc gatgacaatt acgacggcct
    25261 acactaggtt cgcgcctgcc tgcgtgggcg ttccatgcgt caccgtgtct acaatccgga
    25321 ccgcctgaaa tatccgatga aacgagtcgg ggcgcgcggt gaaggcaaat tcgagcgcat
    25381 tagctgggaa gaagcctacg acatcatcgc gaccaatatg cagcgcctga tcaaagagta
    25441 cggcaacgag tctatctatc tgaactatgg caccggtacg ctgggcggca ccatgacccg
    25501 ctcctggccg ccgggaaata ccctagtcgc gcgcctgatg aactgctgcg gcggttatct
    25561 taaccattac ggtgactact cttccgcgca aattgcggaa ggtttgaacc atacctacgg
    25621 cggctgggca gatggtaaca gcccgtcgga tatcgaaaac agtaaattgg tagtgctgtt
    25681 tggtaataac ccaggcgaaa cgcgaatgag tggcggtggg gtgacttact atcttgaaca
    25741 ggcacgccag aaatctaatg ctcgcatgat catcatcgat ccgcgctata ccgacaccgg
    25801 tgccgggcgc gaagatgagt ggatccctat tcgtccagga acagatgccg cactggttaa
    25861 cggtctggcg tacgtcatga tcactaaaaa cctggtggat caggcattcc tcgataaata
    25921 ttgcgttggc tatgacgaga aaaccctgcc cgccagtgcg ccgaaaaatg gtcattataa
    25981 agcttatatt ctgggtgaag ggccagatgg cgtggctaaa acaccggaat gggcctcgca
    26041 aatcaccggt gttccggcag acaaaatcat caaactggct cgtgaaatcg gcagtaccaa
    26101 accggcgttt atcagccagg gatggggccc gcagcgtcac gctaacggtg aaatcgcaac
    26161 ccgtgctatc tcgatgctgg cgattctgac cggtaacgtt ggtattaatg gaggggacag
    26221 cggcgcgcgt gaaggttcat acagcttacc gtttgtccgt atgccgacct tggaaaaccc
    26281 gatccagacc agcatttcga tgtttatgtg gaccgatgcc attgaacgtg gcccggaaat
    26341 gacggcgctg cgtgatggtg tgcgcgggaa agataagctg gatgtgccga tcaaaatgat
    26401 ctggaactat gccggtaact gcctgattaa ccagcattct gaaatcaacc gtacccatga
    26461 aatcctccag gatgataaga agtgcgagct gattgtggtt atcgactgcc acatgacctc
    26521 atcggcgaaa tatgctgaca tcctgctgcc tgactgcacc gcttccgaac agatggactt
    26581 tgcactggat gcatcctgcg ggaatatgtc ttacgtgatt ttcaacgatc aggtgattaa
    26641 accgcgcttt gaatgtaaga ccatctatga aatgaccagc gaactggcaa aacgtcttgg
    26701 cgttgagcaa cagtttactg aaggccgtac ccaggaagag tggatgcggc atctgtatgc
    26761 ccagtcgcgg gaagcgattc ctgaactgcc aacgtttgaa gagttccgca agcaggggat
    26821 ctttaaaaag cgcgacccac aagggcatca cgttgcttat aaagccttcc gtgaagatcc
    26881 gcaggcaaac ccactgacta cgccatcggg caaaattgag atttattcgc aggcgctggc
    26941 tgacattgcc gctacctggg aattgccaga aggcgatgtg atcgatccac tgccgatcta
    27001 cacgccgggc tttgaaagtt atcaggatcc gctgaacaaa cagtatccgc tgcagcttac
    27061 aggtttccac tataaatctc gcgttcactc aacttacggc aacgttgatg tgctgaaagc
    27121 ggcttgccgt caggaaatgt ggatcaaccc gcttgatgcc caaaaacgcg gtatccacaa
    27181 cggcgataaa gtcaggatct ttaacgatcg tggtgaggtt catattgagg cgaaagtgac
    27241 gccacgaatg atgccgggcg tggtcgcact gggtgaaggc gcctggtatg acccggatgc
    27301 aaaacgtgtc gataagggcg gttgtatcaa cgtactgacc actcaacgtc cgtctcctct
    27361 cgctaagggg aatccgtcac atacaaacct tgttcaggtt gaaaaggtgt aaggagtaac
    27421 cgatgacaac ccagtatgga ttttttattg attccagccg ttgcaccggt tgcaaaacct
    27481 gcgagctggc ttgtaaagac tacaaagatt tgacgccaga agtcagtttc cgccgcattt
    27541 atgaatatgc tggcggcgac tggcaggaag ataacggtgt ctggcaccag aacgtgtttg
    27601 cctactatct gtcgatttcg tgcaaccact gcgaagatcc ggcttgtact aaagtctgcc
    27661 cgagcggtgc gatgcataaa cgcgaagatg gttttgttgt ggtcgatgaa gatgtatgca
    27721 ttggctgccg ttactgtcat atggcttgcc cgtatggcgc accgcaatat aacgaaacga
    27781 aaggccatat gaccaaatgc gatggttgtt atgaccgtgt tgcggagggt aaaaagccga
    27841 tctgtgttga atcttgcccg ctgcgggcgc tggatttcgg ccctatcgac gagctgcgta
    27901 aaaaacatgg cgatctggcg gccgttgcgc cgttgccgcg agctcacttt accaaaccga
    27961 atattgtgat caaacccaat gccaatagcc gcccgaccgg ggataccact ggctatctgg
    28021 caaacccgaa ggaggtgtga gatgggaagt ggatggcatg aatggccgct gatgatcttc
    28081 acggtcttcg ggcaatgtgt ggcgggtggt tttatcgttc tggctttggc gctgctcaaa
    28141 ggcgacctgc gagcagaagc ccagcagcgt gttatcgcct gcatgtttgg cttatgggta
    28201 ctgatgggca ttggctttat cgcctctatg ctccatcttg gttcaccgat gcgcgccttt
    28261 aactcgctca accgggtagg ggcttcagca ctcagtaacg aaatcgccag cggttcgatc
    28321 ttttttgctg taggcggcat cggctggttg ctggcaatgc tgaaaaagct gtcaccggca
    28381 ttgcgtacgc tatggctggt ggtgacgatg gttcttggcg tcgtctttgt ctggatgatg
    28441 gtgcgtgtgt ataacagcat tgataccgtt ccgacctggt acagcatctg gacgccgatg
    28501 ggctgggcga tgcgtctgct gccagccatt tctgtactgg cactggtggt gagtggcgtg
    28561 atgtcagtga tgcagggcac agagctggca actattcata gttctgtgca gcaggccgca
    28621 gcgctggtgc cggactatgg tgcgctgatg tcctggcgga tcgtgctttt ggccgttgcc
    28681 ctgtgcttgt ggattgcacc acagctaaaa ggttatcagc ctgcggttcc gctactatcc
    28741 gtctcattca ttctgcttct ggcaggggaa ttaatcggtc gcggcgtatt ctatggtttg
    28801 catatgaccg tggggatggc cgtcgcaagc taatcagtaa caaccggggt ttcggccccg
    28861 gttctctttt atttctgctt cgttaacgtg tcataactgg tcatcaaatt acgataatca
    28921 ggaatatggt tagagaacaa cgtcgccagt ccttcaatat cattacgcca gtcacggtgc
    28981 agctcgcaag ccacgccgaa ccatgtcatc aattgcgcac cagcttgcga catgcgatcc
    29041 cacgccgaat gccgggtaat ttcattaaag gtccctgatg catcagtcac gacaaagaca
    29101 tcaaaccctt cttcaatggc tgaaagcgcc gggaatgcca cgcaaacttc ggttaccaca
    29161 ccggcaataa ttaactgttt tttacctgtc gctttgactg cttttacaaa atcttcgtta
    29221 tcccaggcgt taatattgcc cgggcgagca atgtaaggtg catcggggaa ttgtgctttc
    29281 agttctagaa ctaatgggcc gttaggaccg gtttcaaaac tggtggtaag aatagttggt
    29341 aagttgaaat acttagccag gtcacccagc gccagcacgt tatttttaaa cttatcgggt
    29401 tcgatatccc gtacaaggga aagtaaacca gcctggtgat caacaagcaa aacggcagca
    29461 tcatttttat caagacgaac atacggtttg gtcatcctct tctcctttcg agagtcagtg
    29521 gtacgtctga aatcggtcag acgcgatgca ttgctctgaa agcatagacg ggaaatatga
    29581 gtttgctgtg accatgaaat ttttcgactg aacgaagcgt atgaaatttg tgttagatca
    29641 ataaaaacaa tcagatgaga ttaatcatct cttcagcatt agcgttatga atgagtaata
    29701 taaatcgata ataccgccgc tgcgatatgc gcgtattttc acccattgac aatgtttttg
    29761 gcggtggcat gatgcgcatg aaatttgaac ttcctcacgg ttttaattca tgtccacgta
    29821 tacccggcct gtcatgcttt tgctgtctgg cctgcttttg ttgactctgg cgatcgcggt
    29881 gttaaataca ctcgtgccgc tttggctcgc ccaggaacac atgtccacat ggcaggtagg
    29941 cgttgtcagc tcatcctatt ttaccggcaa ccttgtcggt acattgctga cagggtatgt
    30001 cattaaacgc attggcttta accgcagcta ttatctgtcc tccttcattt ttgctgctgg
    30061 ctgtgccggc cttggcctga tgattggttt ctggagctgg ttggcgtggc gttttgtcgc
    30121 gggcgtcggc tgtgccatga tttgggtggt tgttgagagc gcgctgatgt gcagtgggac
    30181 gtcacgtaac cgtgggcgtt tgcttgctgc gtatatgatg gtttattacg tgggaacgtt
    30241 tttaggccag ttactggtca gcaaagtctc aaccgagctg atgagcgtat tgccgtgggt
    30301 tacaggtttg acattggcag ggatcttacc gctgttgttt acgcgtgtcc tgaatcagca
    30361 ggctgaaaac catgattcga cgtcaattac ggcaatgcta aaactccgtc aggcgcggct
    30421 tggcgtgaat ggctgcatta tctcaggaat cgttctggga tctctatatg gcctgatgcc
    30481 tctgtacctc aatcacaaag gggtgagcaa tgccagtatt ggtttctgga tggcggtact
    30541 ggtcagtgcg ggtatcctcg gacaatggcc gattggacgt ctggcggata agtttggtcg
    30601 actgctggtt ttgcgtgttc aggtctttgt cgtcattctc ggcagtattg cgatgcttag
    30661 ccaggcggcg atggccccag cgttattcat cctcggtgcc gctggcttta cgctatatcc
    30721 ggtggcgatg gcctgggctt gtgagaaagt tgaacatcat caactggtgg cgatgaacca
    30781 agccttactg ttgagctata ccgtgggaag tctgcttggc ccgtcattta gcgctatgct
    30841 aatgcagaat ttctccgata atttattgtt tatcatgatc gccagcgtat cgtttatcta
    30901 tttgctgatg ctgcttcgca acgccggtca tacgccgaaa cccgttgctc acgtgtaaat
    30961 gaattcaagc aaagtgtgaa cttactgttt cacactctgc ttttttgttt cttctatctg
    31021 acttgctttt ttccaaattt tattcgttta aaaataaaat gtgcagcagg ttataatttt
    31081 gcatttcgct atttccgcac ttcttatttg ccgcgcataa tccctcgttt tacagatgcc
    31141 cctttaattt tggcgaagga tttgtctatg gctgggaatg ttcagga
//
LOCUS       DAEBXN010000038        31154 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.038, whole genome shotgun sequence.
ACCESSION   DAEBXN010000038 DAEBXN010000000
VERSION     DAEBXN010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31154
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.038"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(461..1390)
                     /gene="virB"
                     /locus_tag="KJL99_001828"
     CDS             complement(461..1390)
                     /gene="virB"
                     /locus_tag="KJL99_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HBD4436576.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYYKGLEKANESLSSTLPILK
                     EEIKDLDTNLPPDIYKKEILNIIKKSKNRKQNPSLKVDSLFISKDKRTYIKRKENKTN
                     RTLIFTLSKINKTVQREIDEAIRDIISRHLSSS"
     gene            complement(1607..1843)
                     /locus_tag="KJL99_001829"
     CDS             complement(1607..1843)
                     /locus_tag="KJL99_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBD4436577.1"
                     /translation="MIKEKILSIVAFCYGIAYSKLSEETKFIEDLSADSLSLIEMLDM
                     ISFEFNLRIDESTLEHIITIGDLISVVKNSTKSI"
     gene            complement(1847..3748)
                     /gene="ipaA"
                     /locus_tag="KJL99_001830"
     CDS             complement(1847..3748)
                     /gene="ipaA"
                     /locus_tag="KJL99_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HBD4436578.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKENFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNGLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLVDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDTVQTFANNSALEKAFNHTPDN
                     SDGIGSDLLTTSSQERSANNSLSRGHRPLNIQNSSTTPPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQTDFLSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANIRNYVTETNADTIDKNHAI
                     YEKAKEVSSALSKVLSKIDDTSAELLTDDISDLKNNNDITAENNNIYKAAKDVTTSLS
                     KVLKNINKD"
     gene            complement(3757..4755)
                     /gene="ipaD"
                     /locus_tag="KJL99_001831"
     CDS             complement(3757..4755)
                     /gene="ipaD"
                     /locus_tag="KJL99_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HBD4436579.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNQALKKELSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDILSRH
                     EYPINKDARELLHSAPKEAELDGDQMISHRELWAKIANSINDINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVNSLKKALEELKEKYKDKPLYPANNTV
                     SQEQANKWLTELGGTIGKVSQKNGGYVVSINMTPIDNMLKSLDNLGGNGEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            complement(4806..5897)
                     /gene="ipaC"
                     /locus_tag="KJL99_001832"
     CDS             complement(4806..5897)
                     /gene="ipaC"
                     /locus_tag="KJL99_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HBD4436580.1"
                     /translation="MEIQNTKPTQILYTDISTKQTQSSSETQKSQNYQQIAAHIPLNV
                     GKNPVLTTTLNDDQLLKLSEQVQHDSEIIARLTDKKMKDLSEMSHTLTPENTLDISSL
                     SSNAVSLIISVAVLLSALRTAETKLGSQLSLIAFDATKSAAENIVRQGLAALSSSITG
                     AVTQVGITGIGAKKTHSGISDQKGALRKNLATAQSLEKELAGSKLGLNKQIDTNITSP
                     QTNSSTKFLGKNKLAPDNISLSTEHKTSLSSPDISLQDKIDTQRRTYELNTLSAQQKQ
                     NIGRATMETSAVAGNISTSGGRYASALEEEEQLISQASSKQAEEASQVSKEASQATNQ
                     LIQKLLNIIDSINQSKNSTASQIAGNIRA"
     gene            complement(5917..7659)
                     /gene="ipaB"
                     /locus_tag="KJL99_001833"
     CDS             complement(5917..7659)
                     /gene="ipaB"
                     /locus_tag="KJL99_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HBD4436581.1"
                     /translation="MHNVSTTTTGFPLAKILASTELGDNTIQAANDAANKLFSLTIAD
                     LTANQNINTTNAHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINQ
                     IQTRLSELDPESPEKKKLSREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLE
                     KEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQ
                     SLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASL
                     ALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVD
                     SKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFL
                     KNFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGS
                     VASAVFQNSASTNLADLTLSKYQVEQLSKYISEAIEKFGQLQEVIADLLASMSNSQAN
                     RTDVAKAILQQTTA"
     gene            complement(7665..8132)
                     /gene="ipgC"
                     /locus_tag="KJL99_001834"
     CDS             complement(7665..8132)
                     /gene="ipgC"
                     /locus_tag="KJL99_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone IpgC"
                     /protein_id="HBD4436582.1"
                     /translation="MSLNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAY
                     DFYNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKN
                     DYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            complement(8189..8815)
                     /gene="ipgB1"
                     /locus_tag="KJL99_001835"
     CDS             complement(8189..8815)
                     /gene="ipgB1"
                     /locus_tag="KJL99_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB1"
                     /protein_id="HBD4436583.1"
                     /translation="MQILNKILPQVEFAIPRPSFDSLSRNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDSVIDKIKDSNSGNQLFCWMSQERTTYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSDMD
                     LFNTQMKDLTNLVSSSVYDKIFNESTKVLQIEISAEVLKAVYRQSNTN"
     gene            complement(8831..9220)
                     /gene="ipgA"
                     /locus_tag="KJL99_001836"
     CDS             complement(8831..9220)
                     /gene="ipgA"
                     /locus_tag="KJL99_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HBD4436584.1"
                     /translation="MCRKLYDKLYEITGAKLDFNDKNQAFILLEEQIPVCITDNDEYI
                     FLTGLLNEHELFTENIINPEHILILNYSLSRDYGSSICLLPDTHQCVLTKKHYKKYLS
                     PDELIESLYEFLFCIKLTIANITSEVN"
     gene            complement(9233..10717)
                     /gene="icsB"
                     /locus_tag="KJL99_001837"
     CDS             complement(9233..10717)
                     /gene="icsB"
                     /locus_tag="KJL99_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HBD4436585.1"
                     /translation="MILKISNFIDASNTKGPIRVEDTEHGPILIAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLENQKSLLLFLNTLASEKSAESAFAAYEAAKNSIQHSF
                     TGRDIKLMLNTAERFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGNVTKDTEINYLFEKRSGYSVDTYKQDKLNMISDQTAERLDAGQEVRNLL
                     NSKQDQNNNKKIFFPRANQKKDPYGYWGVSADKVYIPLSGDNKTKDGKISHNLFGLDE
                     TNMSKFICKKKADAFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATP
                     NDVYAYANKVRQRIESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHTPK
                     TFKPLSISLYKHPTENVSSEDFDAVINACHSYLVKSAPSNMTRVLNELKTEATDKKEE
                     IIEKSIKIIDYYNSLKSPDLGTKLYIHDLLQINKLLLNNSHSNI"
     gene            11031..12647
                     /locus_tag="KJL99_001838"
     CDS             11031..12647
                     /locus_tag="KJL99_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector inositol
                     phosphate phosphatase"
                     /protein_id="HBD4436586.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGKHKGVSVISYQRVKNGER
                     NKGIEALNRLYLQNQTSLTGKSLLFARDRAGVFCEAIKLAGGDTSKIKAMMERLDTYK
                     LGEVNKRHINELNKVISEEIRAQLGIKNKKELQTKIKQIFTDYLNNKNWGPVDKNISH
                     HGKNYGFQLTPASHMKIGNKNIFVKEYNGKGICCASTRESDHIANMWLSKVVDDEGKE
                     IFSGIRHGVISAYGLKKNSSERAVAARNKAEELVSAALYSRPELLSQALSGKTVDLKI
                     VSTSLLTPTSLTGGEESMLKDQVNALKGLNSKRGKPTKLLIRNSDGLLKEVSVNLKVV
                     TFNFGVNELALKMGLGWRNVDKLNDESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKN
                     DVIYLANQIKEIVTKKLQKNDNGEPYKLSQRMTLLAYTIGAVPCWNCKSGKDRTGMQD
                     AEIKREIIRKHETGQFSQLNSKLSSEEKRLFSTILMNSGNMEIQEMNTGVPGNKVMKK
                     LPLSSLELSYSERIGDPKIWNMVKGYSSFV"
     gene            12657..13019
                     /gene="ipgE"
                     /locus_tag="KJL99_001839"
     CDS             12657..13019
                     /gene="ipgE"
                     /locus_tag="KJL99_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HBD4436587.1"
                     /translation="MEDLADVICRALGIPLIDIDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGNLIARLDLTGINEFEDVYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            13019..13477
                     /gene="ipgF"
                     /locus_tag="KJL99_001840"
     CDS             13019..13477
                     /gene="ipgF"
                     /locus_tag="KJL99_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system lytic transglycosylase
                     IpgF"
                     /protein_id="HBD4436588.1"
                     /translation="MSRFVFILLCFIPYLGRADCWDKAGERYNIPSSLLKAIAEKESG
                     FNKSAVNVNNNGSKDYGIMQINDFHSKRLREMGYSEEMLISHPCLSVHYAAKLLNEFM
                     MMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYRRYLRIAAESKQNNRRI"
     gene            13486..14601
                     /gene="mxiG"
                     /locus_tag="KJL99_001841"
     CDS             13486..14601
                     /gene="mxiG"
                     /locus_tag="KJL99_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HBD4436589.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIYLTISKDNICQFSDEKGEQIDINSQFNSFEYDGIS
                     FHLKNMREDKSRGHILNGMYKNHSVFFFFAVIVVLIIIFSLSLKKDEVKEIAEIIDDK
                     RYGIVNTGQCNYILAETQNDAVWASVALNKTGFTKCRYILVSNKEINRIQQYINQRFP
                     FINLYVLNLVSDKAELLVFLSKERNSSKDTELDKLKNALIVEFPYIKNIKFNYLSDHN
                     ARGDAKGIFTKVNVQYKEICENNKVTYSVREELTDEKLELINRLISEHKNIYGDQYIE
                     FSVLLIDDDFKGKSYLNSKDSYVMLNDKHWFFLDKNK"
     gene            14609..14860
                     /gene="mxiH"
                     /locus_tag="KJL99_001842"
     CDS             14609..14860
                     /gene="mxiH"
                     /locus_tag="KJL99_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle filament
                     protein MxiH"
                     /protein_id="HBD4436590.1"
                     /translation="MSVTVPNDDWTLSSLSETFDDGTQTLQGELTLALDKLAKNPSNP
                     QLLAEYQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNFR"
     gene            14873..15166
                     /gene="mxiI"
                     /locus_tag="KJL99_001843"
     CDS             14873..15166
                     /gene="mxiI"
                     /locus_tag="KJL99_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HBD4436591.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSNPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            15172..15897
                     /gene="mxiJ"
                     /locus_tag="KJL99_001844"
     CDS             15172..15897
                     /gene="mxiJ"
                     /locus_tag="KJL99_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctJ family type III secretion inner membrane
                     ring lipoprotein Mxi"
                     /protein_id="HBD4436592.1"
                     /translation="MIRYKGFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKESE
                     LLVSNIKRFLKNTFSDVKYENISVILTPKEEYVYTNVQPVKEVKSEFLTNEVIYLFLG
                     MAVLVVILLVWAFKTGWFKRNKI"
     gene            15894..16421
                     /locus_tag="KJL99_001845"
     CDS             15894..16421
                     /locus_tag="KJL99_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HBD4436593.1"
                     /translation="MIRMDGIYKKYLSIIFDPAFYINRNRLNLPSELLENGVIRSEIN
                     NLIINKYDLNCDIEPLSGVTAMFVANWNLLPAVAYFIGSQESRLINHSEMVISYYGGK
                     ISKQGEAAIRSGFWHLIAWKENISVGIYERINLLFNPIALEGNYTPVERNLSRLNEGM
                     QYAKRHFTGIQTSCL"
     gene            16363..17058
                     /gene="mxiN"
                     /locus_tag="KJL99_001846"
     CDS             16363..17058
                     /gene="mxiN"
                     /locus_tag="KJL99_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa type III secretion system linker protein
                     MxiN"
                     /protein_id="HBD4436594.1"
                     /translation="MKVCNMQKGTLPVSRHHAYDGVVIKRIEKELCKTIKDRDTESKK
                     KAICVIKEATKKAESLRIDAVCDGYQIGIQTAFEHIIDYICEWKLKQNENRRNIEDYI
                     TSLLSENLHDERIISTLLEQWLSSLRNTVTELKVVLPKCNLALRKKLELDLHKYRSDV
                     KIILKYSEGNNYIFCSGNQVVEFSPQDVISGVKIELAEKLTKNDKKYFKELAHKKLRQ
                     IAEDLLKENPVND"
     gene            17051..17458
                     /gene="mxiM"
                     /locus_tag="KJL99_001847"
     CDS             17051..17458
                     /gene="mxiM"
                     /locus_tag="KJL99_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiM"
                     /protein_id="HBD4436595.1"
                     /translation="MINQINASNALQQRLNSEEVVNLNERLSSSQSFDEDIIYEIMQY
                     FSQSELNSIDNDELHNKIEQLFNSRFPYLTAAQKSSLLNKLIDANQYVDLHEGFYASL
                     SIYNNIDFYIKTTTFDSLISVFEAGREADDSTW"
     gene            17442..17870
                     /gene="mxiM"
                     /locus_tag="KJL99_001848"
     CDS             17442..17870
                     /gene="mxiM"
                     /locus_tag="KJL99_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HBD4436596.1"
                     /translation="MIRHGSNKLKIFILSILLLTLSGCALKSSSNSEKEWHIVPVSKD
                     YFSIPNDLLWSFNTTNKSINVYSKCISGKAVYSFNAGKFMGNFNVKEVDGCFMDAQKI
                     AIDKLFSMLKDGVVLKGNKINDTILIEKDGEVKLKLIRGI"
     gene            18000..18632
                     /gene="mxiE"
                     /locus_tag="KJL99_001849"
     CDS             18000..18632
                     /gene="mxiE"
                     /locus_tag="KJL99_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system transcriptional
                     activator MxiE"
                     /protein_id="HBD4436597.1"
                     /translation="MEGFFFVRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALV
                     EHSHAEKCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLV
                     LYLLRTIEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVF
                     LHNQTITSAAMNNGYASTSHFSNEIKTRLGFSARELSNITFLVKKINEKI"
     gene            18619..20319
                     /gene="mxiD"
                     /locus_tag="KJL99_001850"
     CDS             18619..20319
                     /gene="mxiD"
                     /locus_tag="KJL99_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HBD4436598.1"
                     /translation="MKKFNIKSLTLLIVLLPLIVNANNIDSHLLEQNDIAKYVAQSDT
                     VGSFFERFSALLNYPIVVSKQAAKKRISGEFDLSNPEEMLEKLTLLVGLIWYKDGNAL
                     YIYDSGELISKVILLENISLNYLIQYLKDANLYDHRYPIRGNISDKTFYISGPPALVE
                     LVANTATLLDKQVSSIGTDKVNFGVIKLKNTFVSDRTYNMRGEDIVIPGVATVVERLL
                     NNGKALSNRQAQNDPMPPFNITQKVSEDSNDFSFSSVTNSSILEDVSLIAYPETNSIL
                     VKGNDQQIQIIRDIITQLDIAKRHIELSLWIIDIDKSELNNLGVNWQGTASFGDSFGA
                     SFNMSSSASISTLDGNKFIASVMALNQKKKANVVSRPVILTQENIPAIFDNNRTFYVS
                     LVGERNSSLEHVTYGTLINVIPRFSSRGQIEMSLTIEDGTGNSQSNYNYNNENTSVLP
                     EVGRTKISTIARVPQGKSLLIGGYTHETNSNEIISIPFLSSIPVIGNVFKYKTSNISN
                     IVRVFLIQPREIKESSYYNTAEYKSLISEREIQKTTQIIPSETTLLEDEKSLVSYLNY
                     "
     gene            20338..21405
                     /gene="mxiC"
                     /locus_tag="KJL99_001851"
     CDS             20338..21405
                     /gene="mxiC"
                     /locus_tag="KJL99_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HBD4436599.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDRDFIDRLKRYFKDPSDQVLALRELLNEKDLTAEQVELLTKIINEIISGSEKSVNAG
                     INSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYIEWLGNFGFNHRHTIVNFV
                     EQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIFMKKLESSSLLKDGSLSAE
                     QLLLTLLYIFQYPSESEQILTSVIEVSRASHEDSVVYQTYLSSVNESPHDIFKSESER
                     EIAINILRELVTSAYKKELSR"
     gene            21418..23478
                     /gene="mxiA"
                     /locus_tag="KJL99_001852"
                     /gene_synonym="virH"
     CDS             21418..23478
                     /gene="mxiA"
                     /locus_tag="KJL99_001852"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein MxiA"
                     /protein_id="HBD4436600.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKINANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSFNIYFDKVCITDENGDIDALGIPVVST
                     SYNERVISWVDVSYTENLTNIDAKIKSAQDEFYHQLSQALLNNINEIFGIQETKNMLD
                     QFENRYPDLLKEVFRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVIT
                     LVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDE
                     VMETLAHALRELRNAKKNFVLLVSVDIRRFVKRLIDNRFKSILVISYAEIDEAYTINV
                     LKTI"
     gene            23491..23892
                     /gene="spa15"
                     /locus_tag="KJL99_001853"
                     /gene_synonym="spaK"
     CDS             23491..23892
                     /gene="spa15"
                     /locus_tag="KJL99_001853"
                     /gene_synonym="spaK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone Spa15/SpaK"
                     /protein_id="HBD4436601.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRV
                     VIKDDYVHDGIVFAEILHEFYQRMEILNGVL"
     gene            23896..25188
                     /gene="spaL"
                     /locus_tag="KJL99_001854"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
     CDS             23896..25188
                     /gene="spaL"
                     /locus_tag="KJL99_001854"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SpaL"
                     /protein_id="HBD4436602.1"
                     /translation="MSYTKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IVARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGIKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKSRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMAAAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELIGETIR"
     gene            25281..25619
                     /gene="spa13"
                     /locus_tag="KJL99_001855"
                     /gene_synonym="spaM"
     CDS             25281..25619
                     /gene="spa13"
                     /locus_tag="KJL99_001855"
                     /gene_synonym="spaM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Not similar to SpaM from Salmonella T3SS; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein Spa13"
                     /protein_id="HBD4436603.1"
                     /translation="MEALDKRIIYFLQLENDLEPVGAQSVSQLFNTRRKIAIVKKHII
                     QYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLILDEL
                     SQEDMKYGIR"
     gene            25606..26484
                     /gene="spa32"
                     /locus_tag="KJL99_001856"
     CDS             25606..26484
                     /gene="spa32"
                     /locus_tag="KJL99_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HBD4436604.1"
                     /translation="MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEY
                     SLIASNYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQ
                     DIKAREKINIGDMRGIFSYNKSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHS
                     LSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDKNVFQKNSE
                     MTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSSQHNKG
                     NKNNSTGYNEQSEEEC"
     gene            26478..27359
                     /gene="spa33"
                     /locus_tag="KJL99_001857"
                     /gene_synonym="spaO"
     CDS             26478..27359
                     /gene="spa33"
                     /locus_tag="KJL99_001857"
                     /gene_synonym="spaO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctQ family type III secretion system ring
                     protein Spa33"
                     /protein_id="HBD4436605.1"
                     /translation="MLRIKHFDANEKLQILYAKQLCERFSIQTFKNKFTGSESLVTLT
                     SVCGDWVIRIDTLSFLKKKYEVFSGFSTQESLLHLSKCVFIESSSVFSIPELSDKITF
                     RITNEIQYATTGSHLCCFSSSLGIIYFDKMPVLRNQVSLDSLHHLLEFCLGSSNVRLA
                     TLKRIRTGDIIIVQKLYNLLLCNQVIIGDYIVNDNNEAKINLSESNGESEHTEVSLAL
                     FNYDDINVKVDFILLEKNMTINELKMYVENELFKFPDDIVKHVNIKVNGSLVGHGELV
                     SIEDGYGIEISSWMVKE"
     gene            27364..28014
                     /gene="spa24"
                     /locus_tag="KJL99_001858"
                     /gene_synonym="spaP"
     CDS             27364..28014
                     /gene="spa24"
                     /locus_tag="KJL99_001858"
                     /gene_synonym="spaP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa24/SpaP"
                     /protein_id="HBD4436606.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLMEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FVVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINIPA"
     gene            28029..28289
                     /gene="spaQ"
                     /locus_tag="KJL99_001859"
     CDS             28029..28289
                     /gene="spaQ"
                     /locus_tag="KJL99_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HBD4436607.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLSIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            28289..29059
                     /gene="spa29"
                     /locus_tag="KJL99_001860"
                     /gene_synonym="spaR"
     CDS             28289..29059
                     /gene="spa29"
                     /locus_tag="KJL99_001860"
                     /gene_synonym="spaR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa29/SpaR"
                     /protein_id="HBD4436608.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVLLGVLSRFAPQMNAFSVSLT
                     IKSLLAIFIIFICSSTIYFSKVQFFLGEHKFFTNLFVR"
     gene            29066..30094
                     /gene="spa40"
                     /locus_tag="KJL99_001861"
                     /gene_synonym="spaS"
     CDS             29066..30094
                     /gene="spa40"
                     /locus_tag="KJL99_001861"
                     /gene_synonym="spaS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein Spa40/SpaS"
                     /protein_id="HBD4436609.1"
                     /translation="MANKTEKPTPKKLKDAAKKGQSFKFKDLTTVVIILVGTFTIISF
                     FSLSDVMLLYRYVIINDFEINEGKYFFAVVIVFFKIIGFPLFFCVLSAVLPTLVQTKF
                     VLATKAIKIDFSVLNPVKGLKKIFSIKTIKEFFKSILLLIILALTTYFFWINDRKIIF
                     SQVFSSVDGLYLIWGRLFKDIILFFLAFSILVIILDFVIEFILYMKDMMMDKQEIKRE
                     YIEQEGHFETKSRRRELHIEILSEQTKSDIRNSKLVVMNPTHIAIGIYFNPEIAPAPF
                     ISLIETNQCALAVRKYANEVGIPTVRDVKLARKLYKTHTKYSFVDFEHLDEVLRLIVW
                     LEQVENTH"
     gene            30107..30349
                     /locus_tag="KJL99_001862"
     CDS             30107..30349
                     /locus_tag="KJL99_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4436610.1"
                     /translation="MCYMGVNFCNKIGIDQSEFEIESSIINSIANEVLNPISFLSNKD
                     IINVLLRKISSECDLVRKDIYRCALELVVEKTPDDL"
     gene            complement(30956..31154)
                     /locus_tag="KJL99_001863"
                     /pseudo
     CDS             complement(30956..31154)
                     /locus_tag="KJL99_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT         9734 a         4530 c         6140 g        10750 t
ORIGIN      
        1 gagaataaaa cagtaattat atttgagaaa tctgctaata tattcttaaa gcttttttga
       61 tactgtgaac gaagtctgtt aatggtggat tttttgttta tcaccatgta aaaaaaattg
      121 agtagtaaaa gcgttgactg ctaatcctcg ctgtttttat tgaaacgatt aaaaatacgc
      181 tgaatatttt cggccagtca ctcatattca cataaaattt tgaccatcat gccgcatccc
      241 ttagttgcac aattcatttt caatgatatg aatattacta atttcaccag ataattttct
      301 cgtggtcagc agggtatgag ttttcaccgt aattttaagg aaacacagtg cgtggggatt
      361 gaataacaga tagccgacgg aattacccca ccggctgaaa gtcaagttca acgaagattt
      421 ttattatctg aattgggcag tttacatcag tgttcgatgt ttatgaagac gatagatggc
      481 gagaaattat atcccgaata gcttcatcta tttctctctg aactgttttg tttattttag
      541 ataatgtaaa tattagagtt ctgtttgttt tattctcttt tctttttatg taagtccgtt
      601 tgtctttaga aataaataac gagtcaactt ttagcgaagg gttttgcttt ctgtttttgc
      661 ttttctttat gatgtttaaa atttcttttt tatatatgtc cgggggcaaa tttgtatcaa
      721 ggtcttttat ttcttccttt aatattggta gtgtagaact aagagattca ttagcctttt
      781 caagtccttt ataataattg aataatatct tgtagtcatt aaagttaagt tctgacgcga
      841 ttggaaatag agatattatt tcctgtggaa cgcttgctgc ctgaaaggca cgagtgactt
      901 tcgcgcgaga cagattctct tttttggcta tgtctttata ggacatccct gatactttca
      961 gaaaattaag accaatacca agttctcgga tgctatgctc ttttgctgtt tgtatatcgt
     1021 ttgctagttt tctggcatca agagtagata tatattcttt tgaatataga acttcaagat
     1081 ctgctcctgc atatattgca gatgctcttc tacgagtgcc atccagaatt tcaattctac
     1141 catcaatctc ccttcctatt acagggaaga attgttgtag ctttatagtt tttatgatat
     1201 cggctaatga ttcttctgtt agggattctt gatcgcgtac attcgttttt tggttgacga
     1261 aggttaaatc ttggatcctt ttatgaggaa tcttggcttt gataaaggaa actttattac
     1321 cagaatggag tgtaaactct ttcttttggc cttcctttat acttaacaag tcgttgcaca
     1381 aatccaccat atcacaccct gtttattcat attgatttat attgacaaaa gttaaatgca
     1441 gtgtgagcaa gattgtatgt aactgttgat gatagtcaac ggtagtctta aaaattacta
     1501 ttttaatagc tggattgatg caatttatct ttattggata aaagaataat attcttgtgg
     1561 agctctgatg tgagagctcc atagatgaaa aaatgcttat caattactat attgattttg
     1621 tactattttt gactacagaa ataagatctc caatagtaat aatgtgttct aatgtagatt
     1681 catctatacg tagattgaac tcaaaactaa tcatatccag catttcaatc agagaaagag
     1741 aatcggcaga aagatcttca ataaatttag tttcttcaga tagtttgctg tacgctatac
     1801 catagcaaaa ggccactatt gataatattt tttcttttat catattttaa tccttattga
     1861 tattctttaa tacttttgat agggaagtgg ttacatcttt tgctgcttta tatatattat
     1921 tgttttcagc tgtaatatca ttgttatttt ttaaatcaga tatatcatct gtaagtaatt
     1981 ctgcagaggt atcgtcaatt tttgacaata ccttgctgag ggcgctagat acttcttttg
     2041 ccttttcata gatcgcatga tttttatcaa ttgtatctgc gttggtttct gttacgtagt
     2101 ttcttatatt tgcatctgta acttttaatt tgttgtttat tctatgttct gttgtggatt
     2161 ttgtaatgtc ctttgatttt tcctgtaact ctcgttcttg tgttcctgat ttagttccaa
     2221 tgagagatat aagattactt agggagtttg ttactttttt tgaagcttca aatatcgttt
     2281 cccgtgttaa atatgtgtct ccatttcttg ataaaaaatc agtctgaagt acttttgaat
     2341 cagtgtttga gttgaattta ttgatttgcg aagctactct atgagaaaga gaagcgctac
     2401 ttttagttgt atctgatgag ttatcattac tgcttgtcac tccttccggg tggagagggg
     2461 gggtggttga agagttctgt atgttcagag gcctgtgtcc ccgagaaaga gagttatttg
     2521 cagatctttc ttgactactc gtagttaaca ggtctgaacc tattccatcc gaattatctg
     2581 gagtatggtt gaaagccttt tctaaagctg aattatttgc aaatgtctgg acagtatctg
     2641 atatagagtg tggtagaacg gaagttactt tttgaactag tgctctttgt ggatttagta
     2701 atgagtttcc gttacgggat aatagatcgt ttgtctgaga agtaggagac tgtgcatcat
     2761 tttctgggtt ctcgtgatag ctattgtcat atactctgtt gtcaaaaact ctattatcgt
     2821 aagttctatt atcattattt atatgatagt gaattacagg ttgggatgtt ttctcattgg
     2881 ctccgggggt tggtccgcca tctggcatat cttcgggagg tgtagggagg ccatcttgga
     2941 taggatgcga atcttttata tcttctaaaa tatcattaag gacttttctc aacagctcag
     3001 gaaacacggc tactgtattt aagtctcttg acagtttacc tataccaaat cctttactat
     3061 caatagtatt tttacgttga ttgcgcaaac tatcaaaggc aagttttgcc tgtgcgtcaa
     3121 ctaaatcatc tagtttgaga gatgaaacag tttcattact taactttaat tcagcaagaa
     3181 tattattctt gataaatgat gatacttcag tattgaggag tttttgtccc aatgataaag
     3241 cttcaccaga tgtgtgttgt tgtgctaata gtgcaagtct gtgctcaatg ttttgtcgta
     3301 attgatggag tttcttattc tttgccgacc atggtccaaa ggcagaatct tgggataatt
     3361 tatcttttat aattagttcc gaatactgtg cgacaatttt ttgaagtccc ttttcgtttt
     3421 caaaattaag accattttga tgtactaagg agtacaaaac atttgaaaat tcgagaatct
     3481 cgcttttggc atacgcattc attgagttgg aataaatgct tcttaacaca tcttctttcc
     3541 ctgaggaaga aaataaaaaa tcaagacatt tctgattaaa agacgttgca aagttttctt
     3601 tttcagacac tgatgctggt gtttttagag aagtttgcga actatggata tcggatattt
     3661 tgctttttaa ctcgctgtat tctgttgatg atggtgaagt tgccttatat aagaatgttg
     3721 gcgcttgagt attatttaca ttatgcatgg cgcacctcag aaatggagaa aaagtttatc
     3781 tgtatctgta catgagctta ttgtactact caaaaccttt actaaattat caaaaatact
     3841 attggcatta ctgtattttt gaactaaagt ttgaagatta tttttcattg tttcatcttc
     3901 ggcagagaat ccggcattcc atgcctgata ttttgcatta tctagcacaa cctcgccatt
     3961 tccacctaga ttatctaagc tttttaacat attgtctatt ggggtcatgt ttatactgac
     4021 aacatatccc ccgttttttt gagatacctt gccgattgtt ccacctaatt ctgtaagcca
     4081 tttatttgct tgttcctgac taacagtatt atttgctgga tatagcggtt tatctttata
     4141 tttttccttg agttcttcca atgccttttt aagcgagttg acttgtaatt tcacggagtt
     4201 tccgtcgtta cctccgggag agatccagcc ggcaagactg gaaagaacag cgctaaaatc
     4261 ttgatacatt tgagtatatg aactaacggc atgttcatat actttcagat actgttcatt
     4321 aatatcattg atggagtttg caattttagc ccacagttct ctatgagata tcatttgatc
     4381 tccatcaagc tcggcttctt tcggagctga atgtaataat tctcttgcgt ctttattaat
     4441 tggatattcg tgcctggaaa gaatatctaa tagttgagcg gatttattta catccatgct
     4501 aatctgagat gaatgtaatg ctatttcttc tagcgatgtt ttagtcaacg ttttttgtga
     4561 aagctctttc tttaatgcct gatttgttgt tctgatatta tgaagggtgt cgttgagcat
     4621 agtaagggaa cttacaggat gagaactggt cgttgttttt atatcagaat taactgtttc
     4681 ggttgatgaa ccgttggtat tgtttggact gaatgatgag gtggaaatac tattagtcag
     4741 agttgttata ttcatggtta tttccttaca ggaagagcca tatattatta tggctcttgg
     4801 atatattaag ctcgaatgtt accagcaatc tgactggctg tcgaattctt tgattggttg
     4861 atgctgtcaa ttatattcaa taatttttgt attaattgat ttgtcgcttg ggatgcttct
     4921 ttagatactt gggatgcttc ctctgcttgt ttactgctgg cctgactgat tagttgttct
     4981 tcttcttcaa gagcagatgc ataacgccct cctgatgtgg atatattacc agcaacggct
     5041 gatgtttcca ttgttgcacg gccaatgttt tgtttttgct gcgcagaaag ggtattgagc
     5101 tcgtaagttc ttctctgggt gtcaatttta tcctgcaaag aaatatcggg agaactaaga
     5161 gaagttttat gttcagttga cagggatata ttatctggcg ccagtttatt tttacctaaa
     5221 aattttgtgc tagagttagt ttgtggtgag gtgatatttg tatctatttg tttatttaac
     5281 cctaatttag aacctgcaag ctctttttca agagattgag cagtggcaag gttctttctt
     5341 aaggctcctt tttggtcgct aatccctgaa tgcgtttttt tggcaccgat acccgttata
     5401 cctacttgtg tgactgctcc agtaatgctt gatgataggg ctgccaggcc ttgccgaaca
     5461 atgttctctg cagctgattt tgtagcatcg aacgcaatca atgacaattg agagcccaat
     5521 ttagtttctg cagtgcggag agcagaaagt agaacggcta cactaataat taaagaaaca
     5581 gcattagaag aaagactgga aatatccaga gtgttctctg gagtaagggt gtgactcatc
     5641 tctgaaagat ctttcatctt tttgtcagta aggcgagcaa tgatttctga atcatgctga
     5701 acctgctctg ataactttaa aagttgatca tcatttaatg tggttgttaa tacgggattt
     5761 ttaccgacat taagtggaat atgcgctgca atctgctgat aattttgtga tttttgtgtt
     5821 tcggaagaac tttgagtttg ttttgtggat atatctgtat ataaaatctg ggttggtttt
     5881 gtgttttgaa tttccataac attctcctta tttgtatcaa gcagtagttt gttgcaaaat
     5941 tgcttttgca acatcagttc tattagcctg agagttggac attgaggcta atagatctgc
     6001 aattacttcc tgcaattggc cgaatttttc tattgcttca ctgatatatt ttgacagttg
     6061 ttcaacttga tatttcgata atgtcaggtc tgctagattt gtcgacgcgc tgttctggaa
     6121 aacagcagaa gcgacacttc ctcccgcttg tgtggcagag ttaacacttt ctcctaataa
     6181 aactgcttgg tttaaatggg tggaaataat ttgtttggat attacttcat cacccgctgc
     6241 accaagaaat ttatttaatc tggcaacagc attagtgatt aaatcgtcca gttgagaaga
     6301 aaaattcttg agaaactttg gtataaggtc tgtgagggtt ttacctatta ttttgccaat
     6361 attttctgca agttttgctg ctgcctgttt accaacagtg gctacgagaa cgactgctgc
     6421 aactaggaca agagcgcctg cgattgcccc cagaatagag ccaatcattt tggctttttt
     6481 cgagtcgacg cccaagcctt cgagcatttt tgtaaatgca tctgaaagga gtttgattaa
     6541 gggttcaatg actgctttca tgatcggatt cagggcttgt tccatgaagg aattgccggt
     6601 cgctgcttgt actatagcat ccgtaaccat aagagctaaa ccaacagctg ccagtgctag
     6661 agaggctcct ccagaaaaag ctgctgcaac aacactaacg atagttaaaa gtgccccaag
     6721 tattttccca acacaaccca ttactctgtt gagttcttct gctttacgta cttcagcagc
     6781 atactcatca gattttctct ccatttcagt ttttcttgat tcttggagag actggaatag
     6841 agccagatca ttttttaaag attcttcatt attttttcca actagttgaa taaaggttgc
     6901 catcaattga gtaacactgg caagtccggt taatgatttc tgctgggttg atagctgttc
     6961 agcagatgct gtgtttgaaa atgcagaaaa agagtctatt tctttttcga gttgcattga
     7021 tttatctgta agtttgctat gaattgacag ggttttctgc tcaatcaatg tcctgtcttt
     7081 aactgctgcg tcttttttga tagtgagttg tatttcttcc cggcttaatt ttttcttttc
     7141 tggtgactct gggtcgagtt cggataatct tgtttgaatt tggttaattt tattttctag
     7201 gtcttttatt ttagaatctg cgttttttag tttattaatt tgtttttcat agtctctggt
     7261 tagtccttca gtttcagata gaagagtgtt aattttatcg gagaattcta ggtttttttg
     7321 ctgtcttgcc tgttgctggg acttccaagc agtaatttta tttgttaatg cagttaaaga
     7381 tttttcaccg agtatttgaa taaggtttcc aattaaaagc gttagttggg aacttgcatt
     7441 taatgacttt ggtgctttaa gttcagggat taatatattt gaagttgagt gtgcattagt
     7501 tgtattaata ttttggttag cagtaagatc agcaattgta agagaaaata atttgttagc
     7561 tgcatcattt gcagcttgga tagtattgtc tccaagctca gtggaagcca atattttggc
     7621 aagaggaaaa ccagtggttg tggtgcttac attatgcata ataattactc cttgatatcc
     7681 tgaattgcgt ccaagtatga ttgtgctttt atttttaatt tttcatcatt gctgtgttga
     7741 attacgagtt cgaagcactc tttagctttt aagggggctt tcaaccgaag ctggcattgt
     7801 ccagtatgga atactggtgt atagtcattt tttcctaatg caaaagcgac agcataaagg
     7861 tctgctgctt gttggaactg ttcttttatc tgataaatag ctgcgagtcc cataatgtag
     7921 tctacattgt aaaagtcgta tatacataaa aacctgaaga aaacttcagc ttcctctatt
     7981 cttcctttgt tgtaaaagtc ataagcatat gaataaatgt catccatcat atcatcagga
     8041 attgcattaa tatctttcag tgtagcgcca gagttaattg catcaattac tgcagtagag
     8101 atgctttcat tttcggtgat atttaaagac ataaggtctc ctttttgtct gctttacttt
     8161 aataagtata agatttaata taaaagattt aatttgtatt gctttgacgg tatacagcct
     8221 ttagaacctc tgcgcttatt tctatttgta aaacttttgt tgattcatta aatattttat
     8281 cgtagacgga actggatact agatttgtaa gatccttcat ttgtgtgtta aacaaatcca
     8341 tgtcactgct atagcgtttt aaaatctttg ctctgaggcc agatgaggca atctcgttta
     8401 gtgctgtatg actgatagat gtttgtgctg atttttcatc aagttttata tccgggaatt
     8461 ttttttcaat agctgcaaat atgttttttt tgttatctga ggtcaaaaca actccattgt
     8521 gtatggccat ttcatctata cttctgttta tcattgaaga gacataagtg gttcgctctt
     8581 ggctcatcca acaaaatagt tgattccccg aattactatc ttttattttg tctatgacgc
     8641 tgtccctgat tttatttata ttcaccgggc atccaaaatt cttctgagga aagcgttgtt
     8701 ttaagttaaa aacagataat attttcttta caagcttatt gcgacttaac gaatcaaatg
     8761 atgggcgagg aatcgcgaat tcaacctgtg gaagtatttt gtttagaatt tgcatgatac
     8821 cccctatatg ttagttcact tctgaagtga tgtttgcaat ggttagttta atacaaaata
     8881 aaaattcata caaagattca atgagttcat caggtgaaag atattttttg tagtgtttct
     8941 tggtcaaaac acattgatga gtatcaggga gaagacagat agaagaacca taatctctac
     9001 tcaaagagta gttaaggatg agtatgtgtt ctggattaat aatattttct gtaaaaagtt
     9061 catgttcatt aagaagtccg gtcaaaaaga tgtattcatc attatctgtt atacaaacag
     9121 gtatttgttc ttcgagcaat ataaaggctt gatttttatc gttaaaatca agctttgcac
     9181 ctgttatttc ataaagttta tcatatagtt tgcgacacat ttctatggcc ttctatatat
     9241 tagaatgaga gttattcaat aaaagcttat tgatctgtaa taagtcgtga atgtaaagtt
     9301 tggttccaag atctggcgat ttaagagaat tgtaataatc aataattttt attgattttt
     9361 cgatgatttc ttctttttta tctgttgctt cggtttttaa ttcgtttaat acacgagtca
     9421 tattgctggg tgctgatttt actaagtatg agtggcaagc gttgatgacc gcatcaaagt
     9481 cttctgaaga tacattttca gtaggatgtt tgtaaagtga aattgataaa ggcttgaatg
     9541 tttttggtgt gtggataagg ttaattttat tgaaactttt aagatagctg cgtcgtaatt
     9601 gagtcaatct ggaaagatca aaatcagatt caatatactt cattatttcg ttataagact
     9661 gattgagact ttcaatgcgt tgcctaactt tatttgcata cgcgtataca tcgttaggtg
     9721 tagcaaccaa tttaacgtca gggagtggaa agtatatttc agaatttcct gctttaagaa
     9781 cattaagtgc cataccagca cagttttcag atttactgat taatttataa tttgccaatt
     9841 gtctgaaagc atctgctttc tttttgcata taaattttga catgtttgtt tcatcaaggc
     9901 caaaaaggtt atggctgatt ttaccatcct ttgttttatt atcacctgat aatggaatgt
     9961 atactttatc agcactgacc ccccaataac cataggggtc ttttttttga ttagctctgg
    10021 gaaaaaatat ttttttgttg ttattttgat cttgttttga atttaataag tttctaacct
    10081 cttggcctgc atcaagtctt tcggctgttt ggtctgaaat catatttagt ttgtcttgct
    10141 tataggtatc gacactgtaa cctgagcgtt tttcaaaaag gtagtttatt tctgtatctt
    10201 ttgtaacatt cccgccggga taccagctga gataggtatg agttggttcc tgtagtaaat
    10261 tgttttttat actgatacta gtatgcccta aatgggttat gcctctattt ccccagcaac
    10321 gaaaaactaa atgtctttca agatttttgg ctgtaccgat gccatgaaaa cgctctgcgg
    10381 tattcaacat taatttgatg tctctacctg tgaaagagtg ttgaattgag tttttagctg
    10441 cttcataggc ggcaaacgca ctttctgcag atttttcgct agctaatgta ttgaggaaaa
    10501 gcaataagga tttttgattc tctagtcgat aattttttag ttgttcattt aatatctgtg
    10561 aatttatctt tgcattaata gtgcttaatg ttctgaaaaa gagatccttt aggttgaatt
    10621 tctgtgctat caatataggt ccatgctctg tatcttcaac gcgtataggc ccttttgtat
    10681 tgcttgcgtc aatgaaattg ctaattttga ggatcatact ttattaactc tccattactt
    10741 ggtgattgtg gggatgtaaa atgtatttgg tatataatat attcaattaa taaatttaga
    10801 aacttgagcc tgttaacata atcaaatttt cttttgctgt acataatatg tacctcgtga
    10861 gcatatgtag tgctcgtttc atcatgaaat cccacaagat aaagtgcctg atgtatcagg
    10921 ctcggagtgt tatagaaaaa gagagaaccc tgttgaataa gtatcttttt tgttgtaaaa
    10981 cgaggtacaa aactgcaatc aatgccccat ttatgactga ggataattaa atgcacataa
    11041 ctaatttggg attgcatcag gtttcatttc aaagcggaga ttcctataaa ggcgcagaag
    11101 aaaccggaaa gcacaaaggt gtaagcgtga tttcatatca aagagttaaa aatggagaaa
    11161 gaaataaggg gatcgaagcg cttaatcgat tatatttaca gaaccaaaca agcttaacag
    11221 gaaaaagcct tttgtttgcg cgtgacagag ctggagtttt ttgcgaggca ataaagttgg
    11281 caggtggtga tacgtcaaaa attaaagcca tgatggaacg attagatacc tataaacttg
    11341 gtgaagttaa taaaagacat attaatgagc ttaataaagt aataagtgaa gagataagag
    11401 cacagctagg cattaaaaat aaaaaggaat tgcaaacgaa aataaaacaa atatttactg
    11461 attatttaaa taataagaat tggggaccgg tcgacaagaa tattagtcat catgggaaaa
    11521 attatggttt tcaattaact cctgcctctc atatgaagat agggaataaa aatatatttg
    11581 tcaaagagta taatggaaaa ggaatttgtt gtgcttctac gagagagagt gatcatatcg
    11641 cgaatatgtg gctttcaaaa gtggtggatg atgaggggaa ggaaattttt tcgggtatca
    11701 gacatggagt tatatcagcc tatggattga aaaaaaactc atctgaaaga gctgttgctg
    11761 ctcgtaataa agctgaggag ttagtaagcg cagcattata tagcagacca gagttattat
    11821 cacaggcttt gtctggtaaa acagtagatt taaagattgt ttcgacttct cttctgacgc
    11881 cgaccagttt aaccggggga gaggagagta tgttaaaaga tcaggtgaac gcattaaaag
    11941 ggttaaactc taaaaggggg aagccaacga aattattaat tcggaatagt gatggtcttc
    12001 tgaaagaagt aagcgttaat ctaaaagtgg tgacatttaa ctttggtgta aatgaattag
    12061 cgctaaaaat gggcttaggc tggaggaatg ttgacaagct taatgatgaa tcaatatgtt
    12121 ctttgttggg ggataatttc ctcaaaaatg gtgtgattgg aggttgggct gcagaagcaa
    12181 tagaaaaaaa tccaccatgt aagaatgatg tgatatatct ggctaaccag ataaaagaga
    12241 ttgtaaccaa aaagttacaa aaaaatgata atggagagcc gtataaattg tcacaaagaa
    12301 tgactctatt ggcttatact ataggtgctg taccttgctg gaattgtaag agtgggaagg
    12361 acagaacagg tatgcaggat gctgaaatta aaagagaaat aataagaaaa catgaaacag
    12421 gtcagttttc tcaattaaat agtaaattat cctcagaaga gaaaagatta ttttctacta
    12481 ttctaatgaa tagtggtaat atggaaatcc aagagatgaa tactggtgtg cccggaaata
    12541 aagtcatgaa aaaattgccg ctatcttcgt tagagctatc ttattctgaa agaatagggg
    12601 acccaaaaat atggaatatg gtgaaagggt attcgtcatt tgtataagag gaatatatgg
    12661 aagatttagc agatgttatt tgccgagcct tgggtatccc tttaattgat atcgatgatc
    12721 aagcaatcat gcttgatgat gatgtgctaa tatatattga aaaagaagga gattcaataa
    12781 atttattatg ccctttttgt gcattgcctg aaaatataaa tgatcttata tatgcgttga
    12841 gcctaaatta ctcagaaaag atatgcttag ctactgatga tgaaggcgga aatctaattg
    12901 cgcgattaga tcttaccggg ataaatgaat tcgaggatgt atatgttaat acagaatatt
    12961 atatttcgcg tgtgcgttgg ctgaaggatg aatttgcgcg aagaatgaag gggtattaat
    13021 gtcccgtttc gtatttattt tgctgtgttt tatcccatat ttagggagag ctgattgttg
    13081 ggataaggct ggtgaaaggt acaatattcc atcaagtttg ttaaaagcga ttgcggaaaa
    13141 agaatccgga tttaataaat ctgcagtcaa tgttaataac aatggaagta aagattatgg
    13201 tataatgcaa ataaatgact ttcattctaa aagacttaga gaaatgggat attctgagga
    13261 aatgctaatt agtcatccat gtctttctgt acattatgca gcaaagttat taaatgagtt
    13321 tatgatgatg tatggaagag ggtgggaggc tgttggagcc tataatgcag gaacgtctcc
    13381 gaaaaagaaa aaagaaagat taaagtacgc agaggatatc tatagacgct atttgcggat
    13441 agcagcagag agcaagcaga ataatcgaag gatataagag gatttatgtc tgaggcaaag
    13501 aactcaaatc ttgcaccatt caggttatta gtgaaactca caaacggtgt cggggatgag
    13561 tttccgttat attatgggaa taacttaatt gttctcggac ggacgataga aacacttgag
    13621 tttggaaacg acaatttccc agaaaatatt attcctgtaa cagatagtaa atcggatggt
    13681 ataatttatt tgaccataag caaagataat atctgtcaat ttagtgatga aaaaggtgag
    13741 caaattgata tcaattcaca atttaattca tttgaatatg acgggatttc ttttcacctt
    13801 aaaaatatga gggaggataa atcgagaggc catatactaa acggaatgta taagaaccac
    13861 tcggtgtttt ttttctttgc tgttattgtt gtgttaatta taattttttc cctctcttta
    13921 aagaaagatg aagtaaaaga aattgctgaa attatagatg ataagagata tggaatagtc
    13981 aacactggac agtgcaatta tattcttgcc gaaacacaaa atgatgcggt atgggcgtct
    14041 gtagccttaa ataaaaccgg atttaccaaa tgcagatata ttctggtgtc aaacaaagaa
    14101 ataaatagaa ttcaacaata tatcaatcaa cgattcccat ttatcaattt gtatgtgttg
    14161 aatttagtga gtgataaagc ggaattactg gtgtttttaa gtaaagaaag aaatagttca
    14221 aaagataccg aacttgataa attgaaaaat gctctgattg ttgaatttcc gtatatcaaa
    14281 aatattaaat tcaactatct tagcgatcat aatgcaaggg gggatgctaa aggtattttc
    14341 acaaaggtaa atgtccagta taaagagatt tgtgaaaata ataaagttac atattctgtc
    14401 agagaagaac tgacagatga aaagcttgag ctcattaata gactgatttc tgaacataaa
    14461 aatatatatg gagatcagta tattgagttt tctgtacttt tgatagatga tgattttaaa
    14521 ggtaaatcat atcttaacag caaagacagt tatgtaatgt tgaatgataa acactggttt
    14581 tttttagata aaaataagtg aggataaaat gagtgttaca gtaccgaatg atgattggac
    14641 attgagttca ttatctgaaa cttttgatga tggaactcaa acattacaag gtgaactaac
    14701 attggcacta gataaattag ctaaaaatcc ttcgaatcca cagttgctgg ctgaatacca
    14761 aagtaaatta tctgaatata cattatatag gaacgcgcaa tccaatacag tgaaagtgat
    14821 taaggatgtt gatgctgcaa ttattcaaaa cttcagataa tagggagcat tcatgaatta
    14881 catttatcca gtcaatcagg ttgatattat caaagccagt gattttcaat ctcaagagat
    14941 atcaagtctg gaagacgtcg tgtcggctaa atatagtgat attaagatgg atacagatat
    15001 tcaagtatca caaataatgg agatggtaag caatccagaa tcattaaacc cagaatcttt
    15061 ggccaagtta cagacgacgc tctcaaatta ttcaatagga gtatcattag ctggcacgtt
    15121 agcaagaaaa acagtttcgg ctgttgaaac tttattaaag tcttaattta tatgattagg
    15181 tataaaggtt ttattttatt cttgttgctg atgttgattg gatgtgagca acgtgaagag
    15241 ttaatttcta atttatctca aagacaggca aatgaaataa tatctgtgct agaacgccat
    15301 aatattactg ctagaaaagt tgatggaggt aaacagggga tctcggtaca agtcgaaaag
    15361 gggacatttg catcggcagt tgatttgatg cgcatgtacg atttgccaaa tccggagaga
    15421 gttgatatct cacaaatgtt tcctacagat tcattagtgt cttctccaag agctgaaaag
    15481 gcccgtttat atagtgctat tgagcaacgg ctggaacagt ctttagtttc tattggtggt
    15541 gttatttcgg caaaaataca tgttagctat gatcttgaag aaaaaaatat atcttcaaaa
    15601 ccgatgcata tatcagtaat cgctatatat gactcaccga aagagtctga actattagtt
    15661 agtaatatta agcgattttt gaaaaacacc ttttctgatg ttaagtatga aaatatatct
    15721 gtcatattaa ctccgaaaga agaatatgtt tatacaaatg tacaacctgt taaggaagtt
    15781 aaatcggaat ttttaacaaa tgaagtaata tatttatttc tcgggatggc tgtactagtt
    15841 gtcattcttt tggtatgggc attcaaaaca gggtggttca agagaaacaa aatatgataa
    15901 gaatggatgg aatttataaa aaatatcttt caataatttt tgatccagcg ttctatataa
    15961 atagaaatcg gttgaatttg ccttctgaac tgttagaaaa tggcgtaatc agaagtgaga
    16021 ttaataatct cataattaat aaatatgatc taaattgcga tattgaacct ttaagcgggg
    16081 taaccgctat gtttgttgcc aactggaatt tacttccagc tgttgcttat tttatcggta
    16141 gtcaagagtc tcggttaatt aatcatagtg agatggttat tagttattat gggggcaaaa
    16201 tcagtaagca aggggaggcc gctattcgaa gtggtttttg gcatcttatt gcgtggaagg
    16261 aaaatatatc tgttggtata tatgaaagaa taaacttatt atttaatcca attgctttgg
    16321 agggaaatta tacgcctgta gaaagaaatt tgtcccgatt aaatgaaggt atgcaatatg
    16381 caaaaaggca ctttaccggt atccagacat catgcctatg atggtgttgt tataaagaga
    16441 attgaaaagg aattatgtaa aaccataaaa gatagagaca ctgagagtaa gaagaaagca
    16501 atttgtgtta taaaggaggc tacgaaaaaa gctgaatcac tgcgtataga tgctgtatgt
    16561 gatggttatc aaattggtat acagactgct tttgagcata ttattgacta tatttgtgaa
    16621 tggaagttaa agcaaaatga aaatagaaga aatattgagg actatataac tagtttgctt
    16681 tcagaaaatt tacatgatga gaggatcatt agcactttat tggagcagtg gcttagttcg
    16741 ttgcgtaata cagtaacaga attaaaagtg gttttgccca agtgtaattt agcactgagg
    16801 aaaaagttag aacttgatct ccataaatat cgctcagatg tcaagattat tttaaaatat
    16861 tctgaaggga ataattatat attctgtagt ggtaatcaag tagtggagtt tagtcctcag
    16921 gatgtaatat caggtgttaa aatagaactt gcggaaaaat taacaaaaaa tgataaaaaa
    16981 tatttcaaag agttagcaca taagaaactg agacaaattg cagaagactt gttaaaagag
    17041 aatcctgtta atgattaatc aaataaatgc aagcaatgct ctgcagcaaa gattaaatag
    17101 tgaagaagtt gttaatttaa atgagagatt atcatcttcg caatcatttg atgaggatat
    17161 aatctatgaa ataatgcaat atttctcaca gagtgaatta aattccatag ataatgatga
    17221 actgcacaat aagattgaac aattattcaa ttcacgtttt ccttatctta cagctgcaca
    17281 aaaaagttca cttcttaata aactcataga tgcaaatcaa tatgtcgatt tgcatgaggg
    17341 attttatgca tcattgtcca tatataacaa tattgatttc tatattaaga ctacaacctt
    17401 cgattcatta attagtgtct ttgaagcagg gagagaggca gatgattcga catggtagta
    17461 ataagttgaa aatatttatt ttaagtatat tgctattaac actgagtggg tgtgctttaa
    17521 agtcatcatc taattctgaa aaagaatggc atattgttcc tgtaagtaag gattattttt
    17581 ctattccaaa tgatttatta tggtcgttta atacaaccaa taaaagtata aatgtttact
    17641 ctaaatgtat tagtggtaag gcggtttata gttttaatgc aggtaaattc atgggcaact
    17701 ttaatgttaa ggaagtagat gggtgcttca tggatgcaca aaagatagct atagataaac
    17761 tattttctat gctgaaagac ggggttgttt taaaaggtaa taagataaat gataccatcc
    17821 ttatagagaa ggatggggaa gttaaattaa aattaattcg agggatataa ttgtattgtg
    17881 agtaaatata aaggtctaaa tacaagtaat atgttttaca tttactctag tggacatgaa
    17941 ccagttaacg ttgagcttgt aaaagataaa gaacgtaaca taattgagct ggctccagca
    18001 tggaagggtt tttttttgtc cgaaatcaaa atataaaatt cagtgataat gtgaactatc
    18061 attatcgctt taacattaat agttgtgcaa aatttcttgc tttttgggac tatttcagtg
    18121 gtgctctagt agagcatagc catgctgaaa aatgtattca tttctatcat gagaatgatc
    18181 ttcgggatag ttgtaatacc gaatcgatgc ttgataagtt aatgttaaga ttcatctttt
    18241 catctgacca gaatgtaagt aatgcactgg ctatgatacg aatgactgag tcatatcatt
    18301 tggttcttta tcttctgaga actattgaga aagaaaagga ggtaagaata aagagtctga
    18361 cagagcatta tggtgtgtcg gaggcttatt ttagatcttt atgtagaaaa gctttagggg
    18421 caaaagtcaa agaacaactg aacacttggc ggttagtaaa tggtttatta gatgtatttt
    18481 tgcacaatca aacaatcact tcagctgcca tgaataatgg gtatgcatca acatcccatt
    18541 tttcaaatga gataaaaaca cgactaggct tttctgcaag agaattatcg aatattactt
    18601 ttttagtgaa aaaaataaat gaaaaaattt aatattaaat ctttgactct cttgattgta
    18661 ttgttaccat tgatagttaa tgctaataat attgactctc atttattaga acaaaatgac
    18721 atagcgaaat atgttgctca aagtgatacg gtaggatctt tttttgaacg tttttctgcg
    18781 ttattaaatt atcctattgt tgttagcaaa caagctgcaa aaaagagaat atctggggag
    18841 tttgatttat ctaatccaga agaaatgctt gaaaaactga cattacttgt gggactcata
    18901 tggtataaag atggaaatgc attatacata tatgactctg gagaattaat aagcaaagtt
    18961 attttgctgg agaatatatc acttaattat cttattcagt atctgaagga tgctaacctt
    19021 tatgatcaca gatatcctat aagaggaaac attagtgata aaacattcta tatatctggt
    19081 cctccggcat tggttgaact ggtagctaat acagcaactt tgcttgataa gcaggtttct
    19141 tctattggga cagataaagt taattttggg gttataaaat taaaaaatac atttgttagt
    19201 gacagaactt ataatatgag aggggaggat attgtcatcc caggggtggc tactgttgta
    19261 gaacgtttat taaataatgg taaggctctg agtaacagac aagcccaaaa tgatccgatg
    19321 ccacccttta atattacaca gaaagtatct gaggatagta atgacttttc atttagttca
    19381 gttaccaata gttctatatt agaggatgta tcacttattg catatccaga aaccaattct
    19441 atattagtca aaggtaatga tcagcaaata cagattattc gcgatattat cactcagtta
    19501 gatatagcca aacgacatat agaattatca ttatggataa tcgatattga taaatctgaa
    19561 ttaaataacc ttggtgttaa ttggcaagga acggcttcat ttggtgattc gtttggtgcg
    19621 tcatttaata tgagctcatc agcatcgata agcactttgg acggcaataa gtttattgct
    19681 tcagttatgg cattaaatca gaaaaagaaa gcaaatgtag tttcaaggcc ggtgattttg
    19741 acacaagaga atattccggc tatatttgat aataacagga ctttttatgt gtcattagtt
    19801 ggtgagcgca attcttcgtt ggagcatgtt acgtatggaa cattgataaa tgttatacct
    19861 cgcttttcat ctcggggaca aattgagatg tctttaacga tagaggatgg aacaggaaat
    19921 agtcagtcaa attacaatta taataatgaa aatacatctg tccttccaga agttggaagg
    19981 accaaaatca gtacaatagc gagagttcca caaggcaaaa gtttgttgat aggtggttat
    20041 acgcatgaaa cgaactctaa cgaaataata tctattcctt ttctctcctc tattcctgta
    20101 atcggtaatg tgttcaagta taaaacaagt aatatcagta atattgtaag ggtttttctg
    20161 attcaacccc gtgagataaa ggagtcaagt tattataata ctgcagaata taaaagcctc
    20221 atatctgaaa gagaaataca aaagactaca caaataatcc catctgaaac tactttgctg
    20281 gaagacgaaa aatcattggt ttcatactta aattactaac tataaagtag gtgatgtatg
    20341 cttgatgtta aaaatacagg agtttttagc tctgcattca ttgataagtt gaatgcaatg
    20401 acaaattcag atgatggaga tgagactgct gatgcagagc ttgattctgg cttggctaat
    20461 agcaagtata ttgactcatc tgatgagatg gcttccgctc tttcgtcatt tataaacaga
    20521 agagaccttg agaaactgaa aggaacaaat agtgatagtc aggaacgtat tttagatggg
    20581 gaagaagatg aaattaatca caagattttt gatttaaaga gaacgttaaa agataacctt
    20641 cctctagatc gggattttat agacagacta aagagatatt ttaaagatcc aagtgatcaa
    20701 gtcttagcat taagggaact tttgaatgaa aaagatctta ctgctgaaca agtcgaatta
    20761 ttaactaaaa ttattaatga gataatatca ggtagtgaaa aaagtgttaa tgctggaata
    20821 aattcagcta tacaggctaa attatttggc aacaaaatga aacttgaacc acagcttttg
    20881 cgtgcatgtt atcgtggttt tatcatgggg aacatatcaa caacagatca gtatatagaa
    20941 tggcttggta attttggttt taatcacaga catacaattg tgaattttgt agagcagtca
    21001 ctgattgtag acatggattc tgagaaaccg agctgtaatg cttatgagtt tggttttgtg
    21061 ttatctaaat taattgcaat taagatgatt agaacttcag acgtaatttt tatgaagaaa
    21121 ctggaatcct caagcttgct aaaagatggc agtttaagtg cagagcagct attgctaact
    21181 ttattatata tttttcaata tccaagtgaa agtgagcaaa ttcttacttc tgttatagaa
    21241 gtatcacgag ccagtcatga ggattctgta gtgtatcaaa catatctatc ttctgttaat
    21301 gaaagtcctc atgatatatt taaaagtgaa agtgaaagag aaattgcgat caatattcta
    21361 cgagagcttg tcacaagtgc atacaagaaa gagctttcta gataacagga gataaaagtg
    21421 atccagtctt ttcttaagca agtaagtact aagcctgaat taattatatt agtgcttatg
    21481 gttatgatca ttgctatgtt gatcatacca ttacctacat accttgttga ttttttaata
    21541 gggcttaata ttgtacttgc tattctcgtt tttatgggat cattttatat tgaaagaata
    21601 ttgagttttt ctacattccc ttcggttttg ttgataacga cattatttcg tctcgcttta
    21661 tcaataagta ctagtcgact aattttagtt gatgcggatg cgggtaaaat tattactaca
    21721 tttggccagt ttgtcattgg tgatagtctg gctgtaggtt ttgttatttt ttcaatagta
    21781 actgttgtgc agttcattgt tataacaaaa ggatctgaaa gggttgcgga ggtagctgct
    21841 cgcttttctc ttgacggtat gccggggaaa caaatgagca ttgatgcgga tttaaaggct
    21901 ggaattattg atgccgcagg agctaaagaa agacgcagta ttctggaacg tgagagtcaa
    21961 ttgtatggtt catttgatgg agcaatgaag ttcattaaag gtgatgcaat tgctggcatc
    22021 attattattt ttgtcaattt aataggaggt atttctgttg gtatgagtca acatggaatg
    22081 tccctatctg gtgcattatc tacttatacc atactaacta ttggtgacgg attggtctct
    22141 cagattcctg ctctgttaat ttctataagt gcaggattta tcgttacaag agttaatggt
    22201 gatagcgata atatgggacg taacataatg tcccagatat ttgggaatcc ttttgttctc
    22261 attgttacat cagctcttgc cttggcgata gggatgttgc caggttttcc gttttttgtt
    22321 tttttcctga tagcagttac tttgacggct ttattctatt ataaaaaggt cgtagaaaaa
    22381 gaaaaaagtc tgtctgagtc tgattctagt ggctatactg gtacatttga tattgataat
    22441 agccatgact catctttggc aatgatagaa aatctggatg ctattagttc agaaaccgtt
    22501 cctttaattt tattatttgc cgaaaataag ataaatgcaa atgatatgga aggtcttatt
    22561 gaaaggataa gaagtcagtt ctttattgat tatggtgtca ggcttccaac tattttatat
    22621 agaacaagca atgagcttaa ggtcgatgat attgttttgt taataaatga ggtgcgtgcc
    22681 gatagtttta atatatattt tgacaaagtg tgtattacag atgaaaatgg agatatagat
    22741 gctctaggta ttcctgttgt ttcaacttca tataatgaac gtgttatttc ttgggtagac
    22801 gtttcatata cagaaaatct aactaatatt gatgctaaaa ttaaaagtgc tcaagatgaa
    22861 ttttatcacc agttgtcaca agctttatta aacaacataa atgagatttt tggtatacaa
    22921 gaaacaaaaa atatgttaga tcagtttgaa aatcggtatc ctgatctatt aaaggaagtc
    22981 ttccgacatg tgactataca gagaatttct gaggtattac aaagattgct tggagaaaat
    23041 atttctgttc gcaatttaaa acttattatg gagtctttgg cgctttgggc tcccagagaa
    23101 aaagatgtca taacattagt tgaacatgtc cgtgcatcac tttctaggta tatttgtagt
    23161 aaaatagctg tttctggtga gattaaagtt gtgatgcttt ccggatatat tgaggatgca
    23221 ataagaaaag ggataaggca aacctctggt ggctctttct tgaatatgga tatagaggtt
    23281 tcggatgagg taatggaaac tttagcacat gctttgagag aattgagaaa tgcaaaaaaa
    23341 aatttcgttc ttttggtatc agtagatata cgtaggtttg ttaaaagact tatagataac
    23401 agatttaaga gtatactcgt tatatcgtat gctgagattg atgaagcata taccattaat
    23461 gtattaaaga ctatttagtg aggtttaaat atgagtaaca ttaatttagt tcaattagtt
    23521 agagatagtc ttttcacgat tggttgtcct ccatcaatta ttacagattt agacagccat
    23581 tcagcgatta ccatttcttt ggatagtatg cctgctatta atatagctct tgtcaatgag
    23641 caggttatgt tatgggctaa ttttgatgca ccaagtgatg taaaacttca gtcatctgct
    23701 tataacatat taaatttaat gttaatgaac tttagttata gcattaatga gctggtagag
    23761 cttcatcgtt ctgatgagta cttacagtta agagttgtta ttaaagatga ctacgttcac
    23821 gatgggattg tttttgcaga gattctgcat gagttttatc agaggatgga aatcttaaat
    23881 ggggtcttat aatcaatgag ctatacaaaa ttgctcactc aattatcttt tcctaataga
    23941 atctcggggc caatcttgga aacaagtctt agcgatgttt cgattggtga gatttgtaac
    24001 attcaggctg gaattgaaag taatgaaatt gttgcaagag ctcaggttgt aggatttcat
    24061 gatgaaaaaa caatattaag cttgattgga aattctcgtg gactttcacg gcaaacgttg
    24121 attaagccca ctgcccagtt tcttcatacg caagtcggcc gtggattatt gggagcagta
    24181 gtcaatcctt taggggaggt tactgataag tttgctgtta cagataacag tgaaattctt
    24241 tatcgacctg tagataatgc tcctccgcta tatagtgaaa gggctgcaat tgagaagcct
    24301 tttttaacag gtattaaggt tattgattct ttactcacgt gtggtgaagg acagcgaatg
    24361 gggatttttg cgtcagctgg ttgtggcaaa acttttctca tgaatatgct cattgaacat
    24421 agtggtgctg atatatatgt tattgggtta attggtgagc gaggtcgaga ggttactgaa
    24481 acggttgatt atttgaaaaa ctctgagaaa aaaagcaggt gtgttttagt atatgcaact
    24541 tcggattact cttcggttga tcgttgtaat gctgcatata tagccactgc tatagccgaa
    24601 ttttttagga ctgaaggaca taaagtagcg ctttttattg attcattaac aaggtatgcc
    24661 agagcattac gtgatgtggc cttagccgct ggagaatcac ctgccagaag aggctatccg
    24721 gtttcggttt ttgatagctt acccagactt cttgaaaggc caggaaagtt aaaggcaggt
    24781 ggctctatta ctgcatttta cactgttctt ttggaggatg atgattttgc tgatccatta
    24841 gctgaagagg taagatccat tttagatgga catatatatt tgagcagaaa tctagcccaa
    24901 aaaggacaat ttcctgcaat tgattcctta aaaagtataa gcagggtatt tacacaggtt
    24961 gttgatgaaa aacatcgtat tatggccgct gcatttcggg agctactttc tgaaatagaa
    25021 gagctaagga caattattga ctttggtgaa tacaaaccgg gggagaatgc ctctcaggat
    25081 aaaatctaca acaaaatatc tgttgttgag agttttctga agcaagatta tcgactcggt
    25141 tttacttatg agcagacaat ggagcttatt ggtgaaacaa ttagataagg ttttaaaaat
    25201 caaagataaa tatcaacggt ctgttaaatt aattgaagca cacatattaa cactaaaaaa
    25261 aaaactcttt gtatcgagat gtggaggcat tagataaaag gattatatac ttcctgcaac
    25321 tggagaatga cttagaacct gtaggcgctc agagtgttag tcagcttttc aatacaagac
    25381 gtaagatcgc tatagtaaaa aagcatatta tccaatatca gtctgaacga attcttctaa
    25441 agggaagaat tgaagaaatt caaaaagata ttgatgaggc aaatgcttct aaaagaaaac
    25501 tgcttcataa agagagtaaa atatgtaaac ggattggctt gattaaaaga aataactttg
    25561 ccaaacagtt gatacttgat gagttatcac aagaggatat gaagtatggc attagataat
    25621 ataaacctaa attttagtag tgacaaacag atagaaaaat gtgagaaact atcttcaata
    25681 gataatattg atagtctcgt tttgaagaag aagaggaagg ttgaaattcc tgagtactct
    25741 ttaattgcat ctaactattt tactattgat aagcactttg aacataagca tgataaagga
    25801 gaaatttata gtggcattaa aaatgcgttc gaacttagaa acgaacgagc gacatattct
    25861 gatattccag aatcaatggc cattaaagaa aatattttga taccagatca agatatcaaa
    25921 gcaagggaaa aaataaatat cggcgatatg agggggatct tttcatataa taagagtgga
    25981 aatgcagaca agaacttcga aagaagtcat acttcttctg taaaccctga taatctgcta
    26041 gaatctgata atagaaatgg tcaaattggt ttaaaaaatc atagcttgtc tattgataag
    26101 aatattgctg acatcatttc tttactaaat ggaagtgttg ctaaatcatt tgagctgcct
    26161 gtaatgaata aaaatactgc agacataacc ccatccatgt cattgcaaga aaaatcaata
    26221 gttgaaaatg ataaaaatgt ttttcaaaaa aatagtgaaa tgacttacca ctttaaacag
    26281 tggggggctg gacattctgt tagtatttca gtggagtctg gttcttttgt tctaaaaccg
    26341 tcagatcaat ttgtaggaaa taaacttgac ttaattttga aacaagatgc tgagggtaat
    26401 tacagatttg atagcagtca acataataag gggaataaaa ataatagtac aggatataat
    26461 gaacagagtg aagaagaatg ctaagaatta aacattttga cgctaacgaa aaactacaga
    26521 ttttatatgc aaagcaactc tgcgagcgtt tttcgattca gacattcaaa aataaattta
    26581 caggcagtga aagtttagtc actcttactt ccgtgtgtgg ggattgggta attcgtattg
    26641 atacattatc ttttttgaaa aaaaaatacg aggtattttc aggattttct acacaagaat
    26701 ctttactgca tttatcaaaa tgtgtcttta tagagtcgtc atctgtattt tcgattccag
    26761 aactgtctga taagattact ttccggatca cgaatgaaat ccagtatgca actactggga
    26821 gtcatttatg ctgtttttcc tcttctttag gtattattta ttttgacaag atgccggtat
    26881 tacgtaatca agtttctctt gactcattgc atcatctttt agagttttgc ttaggttcat
    26941 ctaatgtaag gctggctact ttaaaaagaa ttcgcactgg tgatataatc atagttcaga
    27001 aactttataa tttattattg tgtaatcaag ttattattgg ggattatatt gtgaatgata
    27061 ataatgaggc aaaaattaat ctgtcagaaa gtaatggtga gtcagaacac acagaagttt
    27121 ctttggcatt attcaattat gatgatatca atgtaaaagt ggactttatt cttttagaaa
    27181 aaaatatgac aatcaatgaa ctaaaaatgt atgtagaaaa cgaattattc aagtttcccg
    27241 atgacatagt taaacatgta aatattaaag taaatggttc tttggttggg catggggaac
    27301 ttgtttctat tgaggatggt tatggtatcg agattagttc ttggatggta aaggagtaat
    27361 ctcatgctga gtgacatgtc cctcatcgct acactttctt tttttacgct attaccattt
    27421 ttagttgctg ctggtacctg ttatataaag ttttctattg tttttgtaat ggtacgaaat
    27481 gcacttggtt tgcagcaggt tccatcaaat atgactctca atggtattgc attgattatg
    27541 gctttgttcg tgatgaaacc cattattgaa gctggttatg aaaattatct aaatggccct
    27601 caaaaatttg atacaatatc tgatattgtc cgtttttctg attctggctt gatggaatat
    27661 aagcaatact tgaaaaagca tacagatctt gagttagcac gttttttcca aaggtctgaa
    27721 gaagaaaatg ctgatttaaa gagtgcagag aataatgatt attcattgtt ttccctcttg
    27781 cctgcatatg cgctaagtga aatcaaagat gcgttcaaaa taggttttta tttatatttg
    27841 ccatttgttg ttgttgatct tgttatttca agcattttac ttgcacttgg tatgatgatg
    27901 atgagtccaa tcacaatatc agtaccaata aaacttgtat tatttgttgc gcttgatggg
    27961 tggggtatcc tgtctaaggc cttgattgaa caatatatca atattcctgc ttagatgatg
    28021 gaggtaatgt gtctgatata gtttatatgg gtaataaggc tctttattta atccttatct
    28081 tttccttatg gccagtaggt atagctacgg ttattggatt atctattggt ttgttacaga
    28141 cagtgactca actccaagag cagacacttc cttttggtat aaagcttata ggtgtctcaa
    28201 tatctttgct acttctttct ggatggtatg gtgaggtttt attgtctttt tgtcatgaaa
    28261 taatgttttt aattaagagt ggggtttgat ggacatttca agctggttcg aaagtattca
    28321 tgtgttttta atactcctga acggcgtttt ttttagattg gctccattat ttttctttct
    28381 tccattttta aataacggta taatttctcc atctattaga atacctgtga tttttcttgt
    28441 tgcatcagga ttaattactt ctggtaaggt agacataggt tcttctgttt ttgaacatgt
    28501 ttatttcctt atgttcaagg aaataattgt tggcctcctt ctctcttttt gcttgtctct
    28561 tcccttttgg atatttcatg ctgttggtag cattattgac aaccagcgtg gggcaacgct
    28621 tagtagttca attgatcctg ccaatggtgt tgatacgtct gagttggcaa aatttttcaa
    28681 tcttttttct gcagttgtat ttctatacag tggtggtatg gtctttattt tagaatccat
    28741 acaattgtct tataatatat gcccgttatt ttctcaatgt tctttccgtg tctcaaatat
    28801 cttaacattt ctgactttat tggcaagtca ggctgttatt ttagccagtc ctgttatgat
    28861 agtattgtta ctatcagaag tattacttgg tgtattatcg agatttgctc cgcagatgaa
    28921 tgctttttcc gtatcattaa ctattaaaag tttacttgca atatttatta ttttcatctg
    28981 ttcttctact atttactttt ctaaagttca atttttcctc ggtgaacata aatttttcac
    29041 aaatctattt gttagataaa atattatggc aaataaaaca gaaaagccga cacctaaaaa
    29101 actaaaggat gccgcaaaaa aaggacagtc atttaaattt aaggatttaa cgactgttgt
    29161 tattattctg gtagggacat ttactataat atcattcttt tccttaagtg atgtaatgct
    29221 cttatacaga tatgtaataa ttaatgactt cgaaattaat gagggtaaat acttttttgc
    29281 agtggttatt gtctttttta agataattgg cttcccactt tttttctgtg ttctttcggc
    29341 tgtgttgcca acattggttc aaacaaagtt tgttcttgcg actaaagcta tcaagattga
    29401 tttttcagta ttaaaccctg ttaaagggtt aaaaaaaata tttagtataa agacaataaa
    29461 agaatttttc aaaagcattc tgcttcttat tattctagca ttaacaacct atttcttttg
    29521 gattaatgac cgaaaaataa ttttttctca ggtgttttct agtgttgatg gcttatatct
    29581 tatttggggg aggctgttta aggatataat attatttttc ttggcatttt ctattcttgt
    29641 tattattctt gactttgtga ttgagttcat tttatacatg aaagatatga tgatggataa
    29701 acaggagata aaaagagaat atatagagca agagggacac tttgagacaa agtcgagaag
    29761 gcgtgagttg catatcgaga ttctttcaga gcagactaaa tctgatatac gtaattcaaa
    29821 attagtggta atgaacccga ctcatattgc aattggtatt tattttaatc cagaaatagc
    29881 gcctgcacct tttatttctc tcattgaaac taaccagtgt gccttggctg tcagaaaata
    29941 tgcaaatgaa gttggtatac cgactgtgcg tgatgtgaaa ttagctagaa aactatataa
    30001 aacacataca aaatatagtt ttgttgattt tgaacacttg gatgaagtcc tacgtcttat
    30061 tgtttggctt gagcaggttg aaaacactca ttaataagta aaggagatgt gttatatggg
    30121 agttaatttc tgtaataaaa taggtattga tcagtctgaa tttgaaatag aatcttccat
    30181 cattaactcc attgctaatg aggtattgaa cccaatatct ttccttagca ataaggatat
    30241 aataaatgtt ttactcagaa agatttcatc tgaatgtgac ctggttagaa aagacattta
    30301 tcgttgtgct ctggagttgg tcgttgaaaa aacacctgat gatttataat cattcataat
    30361 aactcttttg ctgatataaa tatttttgtt gaagatattt atgtagtcac aggatcaatt
    30421 gtagaaaatt gttatgtttt tgttaggaac tgcactaaat aatatatgat aagacagcaa
    30481 aaacgactga ccataatcct tcttttatta ggagttgata agagagacta ttcatcctgt
    30541 aatgttaaaa ctctgttata tagtattcgc gattatgcaa agtctgtaaa tgatcatgag
    30601 atactaaccg agagtaatcg tttattatct cattgcattt cagatagtaa cggagctttc
    30661 tttaaaagta gtaaatacgt tccccttaag tacttacgta aaaggagaat agcacgaaag
    30721 ataccaaatg attagcactt tgctagtaag cttgctggta aactatattg accgttgggt
    30781 ttgttttgag actgtaattt aaattgtgta attgcctgtt tttgatatgt ttactccaac
    30841 aacggagaca ggtaaattat ggacgaaaag aagcttaaag cccttgcggc tgaactggct
    30901 aaaggcctca aaactgaggc cgacctcaat cagttttccc gtatgctgac gaaatatccc
    30961 ggctcttaaa acgatagtgg ctcaggcttt catttttcag cgttccccag aagatttcca
    31021 tcggagcgtt gtcgtaacag ttacctttac gcgacattga tgttttcaga ccagactgct
    31081 cctgtatgac ccggtaatcg tatgcgcagt actgtgaacc tcgagcggag tggtggatta
    31141 gcccggcagg tggg
//
LOCUS       DAEBXN010000039        30666 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.039, whole genome shotgun sequence.
ACCESSION   DAEBXN010000039 DAEBXN010000000
VERSION     DAEBXN010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30666
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.039"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..588
                     /gene="tuf"
                     /locus_tag="KJL99_001864"
     CDS             <1..588
                     /gene="tuf"
                     /locus_tag="KJL99_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBD4436611.1"
                     /translation="IPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEV
                     EIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKP
                     HTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKM
                     VVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            818..1201
                     /gene="secE"
                     /locus_tag="KJL99_001865"
     CDS             818..1201
                     /gene="secE"
                     /locus_tag="KJL99_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HBD4436612.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLAAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATIAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            1203..1748
                     /gene="nusG"
                     /locus_tag="KJL99_001866"
     CDS             1203..1748
                     /gene="nusG"
                     /locus_tag="KJL99_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HBD4436613.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1907..2335
                     /gene="rplK"
                     /locus_tag="KJL99_001867"
     CDS             1907..2335
                     /gene="rplK"
                     /locus_tag="KJL99_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HBD4436614.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            2339..3043
                     /gene="rplA"
                     /locus_tag="KJL99_001868"
     CDS             2339..3043
                     /gene="rplA"
                     /locus_tag="KJL99_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HBD4436615.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3456..3953
                     /gene="rplJ"
                     /locus_tag="KJL99_001869"
     CDS             3456..3953
                     /gene="rplJ"
                     /locus_tag="KJL99_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HBD4436616.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            4020..4385
                     /gene="rplL"
                     /locus_tag="KJL99_001870"
     CDS             4020..4385
                     /gene="rplL"
                     /locus_tag="KJL99_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HBD4436617.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4705..8733
                     /gene="rpoB"
                     /locus_tag="KJL99_001871"
     CDS             4705..8733
                     /gene="rpoB"
                     /locus_tag="KJL99_001871"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBD4436618.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8810..13033
                     /gene="rpoC"
                     /locus_tag="KJL99_001872"
     CDS             8810..13033
                     /gene="rpoC"
                     /locus_tag="KJL99_001872"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HBD4436619.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(13275..14408)
                     /gene="thiH"
                     /locus_tag="KJL99_001873"
     CDS             complement(13275..14408)
                     /gene="thiH"
                     /locus_tag="KJL99_001873"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HBD4436620.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQQAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAVLTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14405..15175)
                     /gene="thiG"
                     /locus_tag="KJL99_001874"
     CDS             complement(14405..15175)
                     /gene="thiG"
                     /locus_tag="KJL99_001874"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HBD4436621.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVALLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHATQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEVGLLARQSGPGSRSYFAHATSPLTGFLEASA"
     gene            complement(15177..15377)
                     /gene="thiS"
                     /locus_tag="KJL99_001875"
     CDS             complement(15177..15377)
                     /gene="thiS"
                     /locus_tag="KJL99_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HBD4436622.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(15361..16116)
                     /gene="thiF"
                     /locus_tag="KJL99_001876"
     CDS             complement(15361..16116)
                     /gene="thiF"
                     /locus_tag="KJL99_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HBD4436623.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQQPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(16109..16744)
                     /gene="thiE"
                     /locus_tag="KJL99_001877"
     CDS             complement(16109..16744)
                     /gene="thiE"
                     /locus_tag="KJL99_001877"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HBD4436624.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16744..18639)
                     /gene="thiC"
                     /locus_tag="KJL99_001878"
     CDS             complement(16744..18639)
                     /gene="thiC"
                     /locus_tag="KJL99_001878"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HBD4436625.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARADTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLHKEEA"
     regulatory      complement(18720..18820)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18873..19349)
                     /gene="rsd"
                     /locus_tag="KJL99_001879"
     CDS             complement(18873..19349)
                     /gene="rsd"
                     /locus_tag="KJL99_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HBD4436626.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19444..20217
                     /gene="nudC"
                     /locus_tag="KJL99_001880"
     CDS             19444..20217
                     /gene="nudC"
                     /locus_tag="KJL99_001880"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HBD4436627.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSLLLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20257..21321
                     /gene="hemE"
                     /locus_tag="KJL99_001881"
     CDS             20257..21321
                     /gene="hemE"
                     /locus_tag="KJL99_001881"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HBD4436628.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRHELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21478..22149
                     /gene="nfi"
                     /locus_tag="KJL99_001882"
     CDS             21478..22149
                     /gene="nfi"
                     /locus_tag="KJL99_001882"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HBD4436629.1"
                     /translation="MDLASLRAQQIELASSVICEDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSKPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22192..22782
                     /locus_tag="KJL99_001883"
     CDS             22192..22782
                     /locus_tag="KJL99_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HBD4436630.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMGDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLRESGESNIGIIFQQ"
     gene            22969..23241
                     /gene="hupA"
                     /locus_tag="KJL99_001884"
     CDS             22969..23241
                     /gene="hupA"
                     /locus_tag="KJL99_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HBD4436631.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23254..23949
                     /locus_tag="KJL99_001885"
     CDS             23254..23949
                     /locus_tag="KJL99_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HBD4436632.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23951..24376)
                     /gene="zraP"
                     /locus_tag="KJL99_001886"
     CDS             complement(23951..24376)
                     /gene="zraP"
                     /locus_tag="KJL99_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HBD4436633.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24614..25990
                     /gene="zraS"
                     /locus_tag="KJL99_001887"
     CDS             24614..25990
                     /gene="zraS"
                     /locus_tag="KJL99_001887"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HBD4436634.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMYR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            25987..27312
                     /gene="zraR"
                     /locus_tag="KJL99_001888"
     CDS             25987..27312
                     /gene="zraR"
                     /locus_tag="KJL99_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HBD4436635.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGAQD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27309..28598)
                     /gene="purD"
                     /locus_tag="KJL99_001889"
     CDS             complement(27309..28598)
                     /gene="purD"
                     /locus_tag="KJL99_001889"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HBD4436636.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFAAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALVYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDNGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLSV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28610..30199)
                     /gene="purH"
                     /locus_tag="KJL99_001890"
     CDS             complement(28610..30199)
                     /gene="purH"
                     /locus_tag="KJL99_001890"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HBD4436637.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7191 a         7982 c         8440 g         7053 t
ORIGIN      
        1 attccggaac cagagcgtgc gattgacaag ccgttcctgc tgccgatcga agacgtattc
       61 tccatctccg gtcgtggtac cgttgttacc ggtcgtgtag aacgcggtat catcaaagtt
      121 ggtgaagaag ttgaaatcgt tggtatcaaa gagactcaga agtctacctg tactggcgtt
      181 gaaatgttcc gcaaactgct ggacgaaggg cgtgctggtg agaacgtagg tgttctgctg
      241 cgtggtatca aacgtgaaga aatcgaacgt ggtcaggtac tggctaagcc gggcaccatc
      301 aagccgcaca ccaagttcga atctgaagtg tacattctgt ccaaagatga aggcggtcgt
      361 catactccgt tcttcaaagg ctaccgtccg cagttctact tccgtactac tgacgtgact
      421 ggtaccatcg aactgccgga aggcgtagag atggtaatgc cgggcgacaa catcaaaatg
      481 gttgttaccc tgatccaccc gatcgcgatg gacgacggtc tgcgtttcgc aatccgtgaa
      541 ggcggccgta ccgttggcgc gggcgttgta gcaaaagttc tgagctaatt gccgataaca
      601 tttgacgcaa tgcgcactaa aagggcatca tttgatgccc tttttgcacg ctttcgtacc
      661 agaacctggc tcatcagtga ttttctttgt cataatcatt gctgagacag gctctgttga
      721 gggcgtataa tccgaaaagc taatacgcgt ttcgatttgg tttgcctcgc gatcgcgggg
      781 tgaaaatgtt tgtagaaaac ttctgacagg ttggtttatg agtgcgaata ccgaagctca
      841 aggaagcggg cgcggcctgg aagcgatgaa gtgggtcgtt gtggtggcat tgctcctggc
      901 ggcgattgtc ggcaactacc tttatcgcga cattatgctg ccgctgcgtg cgctggccgt
      961 agtaattctg attgctgcag cgggtggtgt cgcgctgtta acgacaaaag gtaaagctac
     1021 cattgctttt gcccgtgaag cgcgtaccga agtccgtaag gtcatttggc cgactcgcca
     1081 ggaaacattg cacaccacgc tgattgtggc tgcggttacc gcagtaatgt cactgatcct
     1141 gtggggactg gatggtattc tggttcgcct ggtatccttt atcactggcc tgaggttctg
     1201 agatgtctga agctcctaaa aagcgctggt acgtcgttca ggcgttttcc ggttttgaag
     1261 gccgcgtagc aacgtcgctg cgtgagcata tcaaattaca caacatggaa gatttgtttg
     1321 gtgaagtcat ggtaccaacc gaagaagtgg ttgaaatccg tggcggtcag cgtcgcaaaa
     1381 gcgaacgtaa attcttccct ggctacgtcc tcgttcagat ggtgatgaac gacgcgagct
     1441 ggcacttggt gcgcagcgta ccgcgtgtga tgggcttcat cggcggtact tccgatcgtc
     1501 ctgcgccaat cagcgataaa gaagtcgatg cgattatgaa ccgcctgcag caggttggtg
     1561 ataagccgcg tccgaaaacg ctgtttgaac cgggtgaaat ggtccgtgtt aatgatggtc
     1621 cgttcgctga cttcaacggt gttgttgaag aagtggatta cgagaaatct cgtctgaaag
     1681 tgtctgtttc tatcttcggt cgtgcgaccc cggtagagct ggacttcagc caggttgaaa
     1741 aagcctaacc cagcgatcaa aaaagcggcg atttaatcgt tgcacaaggc gtgagattgg
     1801 aatacaattt cgcgcctttt gtttttatgg gccttgcccg taaaacgatt ttttatatca
     1861 cggggagcct ctcagaggcg ttattaccca acttgaggaa tttataatgg ctaagaaagt
     1921 acaagcctat gtcaagctgc aggttgcagc tggtatggct aacccgagtc cgccagtagg
     1981 tccggctctg ggtcagcagg gcgtaaacat catggaattc tgcaaagcgt tcaacgcaaa
     2041 aactgattcc atcgaaaaag gtctgccgat tccggtagta atcaccgttt acgctgaccg
     2101 ttctttcact ttcgttacca agaccccgcc ggcagcagtt ctgctgaaaa aagcggcagg
     2161 tatcaagtct ggttccggta agccgaacaa agacaaagtg ggtaaaattt cccgcgctca
     2221 gctgcaggaa atcgcgcaga ccaaagctgc cgacatgact ggtgccgaca ttgaagcgat
     2281 gactcgctcc atcgaaggta ctgcacgttc catgggcctg gtagtggagg actaagaaat
     2341 ggctaaactg accaagcgca tgcgtgttat ccgtgagaaa gttgatgcaa ccaaacagta
     2401 cgacatcaac gaagctatcg cactgctgaa agagctggcg actgctaaat tcgtagaaag
     2461 cgtggacgta gctgttaacc tcggcatcga cgctcgtaaa tctgaccaga acgtacgtgg
     2521 tgcaactgta ctgccgcacg gtactggccg ttccgttcgc gtagccgtat ttacccaagg
     2581 tgcaaacgct gaagctgcta aagctgcagg cgcagaactg gtaggtatgg aagatctggc
     2641 tgaccagatc aagaaaggcg aaatgaactt tgacgttgtt attgcttctc cggatgcaat
     2701 gcgcgttgtt ggccagctgg gccaggttct gggtccgcgt ggcctgatgc caaacccgaa
     2761 agtgggtact gtaacaccga acgttgctga agcggttaaa aacgctaaag ctggccaggt
     2821 tcgttaccgt aacgacaaaa acggcatcat ccacaccacc atcggtaaag tggactttga
     2881 cgctgacaaa ctgaaagaaa acctggaagc tctgctggtt gcgctgaaaa aagcaaaacc
     2941 gactcaggcg aaaggcgtgt acatcaagaa agttagcatc tccaccacca tgggtgcagg
     3001 tgttgcagtt gaccaggctg gcctgagcgc ttctgtaaac taatgccttt acgtgggcgg
     3061 tgattttgtc tacaatctta cccccacgta taatgcttaa tgcagacgta tatccgagat
     3121 attcgggttg tggcaaggcg gcaactgagt gagtcgccaa gagcatagct aactatgtga
     3181 ctggtgcgaa tgaaggaagc caacgccgcc acaagttgaa tagcgacgga tagaaaagat
     3241 ttgttcgttg gagcctggcc tatccaggcc tccgtcgaag accgcaggag tttcgcaaga
     3301 aacttaatcc cctgcgtaga cggtgacaga acgctaagat tattctttta tattctggct
     3361 tgtttctgct caccgtaatt aagacgctct ctccgtttgg aggagtgaag tgagttccag
     3421 agattttctc tggcaaacat ccaggagcaa agctaatggc tttaaatctt caagacaaac
     3481 aagcgattgt tgctgaagtc agcgaagtag ccaaaggcgc gctgtctgca gtagttgcgg
     3541 attcccgtgg cgtaactgta gataaaatga ctgaactgcg taaagcaggt cgcgaagctg
     3601 gcgtatacat gcgtgttgtt cgtaacaccc tgctgcgccg tgctgttgaa ggtactccgt
     3661 tcgagtgcct gaaagacgcg tttgttggtc cgaccctgat tgcatactct atggaacacc
     3721 cgggcgctgc tgctcgtctg ttcaaagagt tcgcgaaagc gaatgcaaaa tttgaggtca
     3781 aagccgctgc ctttgaaggt gagctgatcc cggcgtctca gatcgaccgc ctggcaactc
     3841 tgccgaccta cgaagaagca attgcacgcc tgatggcaac catgaaagaa gcttcggctg
     3901 gcaaactggt tcgtactctg gctgctgtac gcgatgcgaa agaagctgct taatcgcagt
     3961 tatcttttta acgcattcgc ttacgtataa acttattctg atattcagga acaatttaaa
     4021 tgtctatcac taaagatcaa atcattgaag cagttgcagc tatgtctgta atggacgttg
     4081 tagaactgat ctctgcaatg gaagaaaaat tcggtgtttc cgctgctgct gctgtagctg
     4141 tagctgctgg cccggttgaa gctgctgaag aaaaaactga attcgacgta attctgaaag
     4201 ctgctggcgc taacaaagtt gctgttatca aagcagtacg tggcgcaact ggcctgggtc
     4261 tgaaagaagc taaagacctg gtagaatctg caccggctgc tctgaaagaa ggcgtgagca
     4321 aagacgacgc agaagcactg aaaaaagctc tggaagaagc tggcgctgaa gttgaagtta
     4381 aataagccaa cccttccggt tgcagcctga gaaatcaggc tgatggctgg tgacttttta
     4441 gtcaccagcc tttttgcgct gtaaggcgcc agtagcgttt cacactgttt gactactgct
     4501 gtgcctttca atgcttgttt ctatcgacga cttaatatac tgcgacagga cgtccgttct
     4561 gtgtaaatcg caatgaaatg gtttaagcgt gatagcaaca ggcattgcgg aaagtgttcc
     4621 attttccggt caacaaaata gtgttgcaca aactgtccgc tcaatggaca gatgggtcga
     4681 cttgtcagcg agctgaggaa ccctatggtt tactcctata ccgagaaaaa acgtattcgt
     4741 aaggattttg gtaaacgtcc acaagttctg gatgtacctt atctcctttc tatccagctt
     4801 gactcgtttc agaaatttat cgagcaagat cctgaagggc agtatggtct ggaagctgct
     4861 ttccgttccg tattcccgat tcagagctac agcggtaatt ccgagctgca atacgtcagc
     4921 taccgccttg gcgaaccggt gtttgacgtc caggaatgtc aaatccgtgg cgtgacctat
     4981 tccgcaccgc tgcgcgttaa actgcgtctg gtgatctatg agcgcgaagc gccggaaggc
     5041 accgtaaaag acattaaaga acaagaagtc tacatgggcg aaattccgct catgacagac
     5101 aacggtacct ttgttatcaa cggtactgag cgtgttatcg tttcccagct gcaccgtagt
     5161 ccgggcgtct tctttgactc cgacaaaggt aaaacccact cttcgggtaa agtgctgtat
     5221 aacgcgcgca tcatccctta ccgtggttcc tggctggact tcgaattcga tccgaaggac
     5281 aacctgttcg tacgtatcga ccgtcgccgt aaactgcctg cgaccatcat tctgcgcgcc
     5341 ctgaactaca ccacagagca gatcctcgac ctgttctttg aaaaagttat ctttgaaatc
     5401 cgtgataaca agctgcagat ggaactggtg ccggaacgcc tgcgtggtga aaccgcatct
     5461 tttgacatcg aagctaacgg taaagtgtac gtagaaaaag gccgccgtat cactgcgcgc
     5521 cacattcgcc agctggaaaa agacgacgtc aaactgatcg aagtcccggt tgagtacatc
     5581 gcaggtaaag tggttgctaa agactatatt gatgagtcta ccggcgagct gatctgcgca
     5641 gcgaacatgg agctgagcct ggatctgctg gctaagctga gccagtctgg tcacaagcgt
     5701 atcgaaacgc tgttcaccaa cgatctggat cacggcccat atatctctga aaccttacgt
     5761 gtcgacccaa ctaacgaccg tctgagcgca ctggtagaaa tctaccgcat gatgcgccct
     5821 ggcgagccgc cgactcgtga agcagctgaa agcctgttcg agaacctgtt cttctccgaa
     5881 gaccgttatg acttgtctgc ggttggtcgt atgaagttca accgttctct gctgcgcgaa
     5941 gaaatcgaag gttccggtat tctgagcaaa gacgacatca ttgatgttat gaaaaagctc
     6001 atcgatatcc gtaacggtaa aggcgaagtc gatgatatcg accacctcgg caaccgtcgt
     6061 atccgttccg ttggcgaaat ggcggaaaac cagttccgcg ttggcctggt acgtgtagag
     6121 cgtgcggtga aagagcgtct gtctctgggc gatctggata ccctgatgcc tcaggatatg
     6181 atcaacgcca agccgatttc cgcagcagtg aaagagttct tcggttccag ccagctgtct
     6241 cagtttatgg accagaacaa cccgctgtct gagattacgc acaaacgtcg tatctccgca
     6301 ctcggcccag gcggtctgac ccgtgaacgt gcaggcttcg aagttcgaga cgtacacccg
     6361 actcactacg gtcgcgtatg tccaatcgaa acccctgaag gtccgaacat cggtctgatc
     6421 aactctctgt ccgtgtacgc acagactaac gaatacggct tccttgagac tccgtatcgt
     6481 aaagtgaccg acggtgttgt aactgacgaa attcactacc tgtctgctat cgaagaaggc
     6541 aactacgtta tcgcccaggc gaactccaac ctggatgaag aaggccactt cgtagaagac
     6601 ctggtaactt gccgtagcaa aggcgaatcc agcttgttca gccgcgacca ggttgactac
     6661 atggacgtat ccacccagca ggtggtatcc gtcggtgcgt ccctgatccc gttcctggaa
     6721 cacgatgacg ccaaccgtgc attgatgggt gcgaatatgc aacgtcaggc cgttccgact
     6781 ctgcgcgctg ataagccgct ggttggtact ggtatggaac gtgctgttgc cgttgactcc
     6841 ggtgtaactg cggtagctaa acgtggtggt gtcgttcagt acgtggatgc ttcccgtatc
     6901 gttatcaaag ttaacgaaga cgagatgtat ccgggtgaag caggtatcga catctacaac
     6961 ctgaccaaat acacccgttc taaccagaac acctgtatca accagatgcc gtgtgtgtct
     7021 ctgggtgaac cggttgaacg tggcgacgtg ctggcagacg gtccgtccac cgacctcggt
     7081 gaactggcgc ttggtcagaa catgcgcgta gcgttcatgc cgtggaatgg ttacaacttc
     7141 gaagactcca tcctcgtatc cgagcgtgtt gttcaggaag accgtttcac caccatccac
     7201 attcaggaac tggcgtgtgt gtcccgtgac accaagctgg gtccggaaga gatcaccgct
     7261 gacatcccga acgtgggtga agctgcgctc tccaaactgg atgaatccgg tatcgtttac
     7321 attggtgcgg aagtgaccgg tggcgacatt ctggttggta aggtaacgcc gaaaggtgaa
     7381 actcagctga ccccagaaga aaaactgctg cgtgcgatct tcggtgagaa agcctctgac
     7441 gttaaagact cttctctgcg cgtaccaaac ggtgtatccg gtacggttat agacgttcag
     7501 gtctttactc gcgatggcgt agaaaaagac aaacgtgcgc tggaaatcga agaaatgcag
     7561 ctcaaacagg cgaagaaaga cctgtctgaa gaactgcaga tcctcgaagc gggtctgttc
     7621 agccgtatcc gtgctgtgct ggtagccggt ggcgttgaag ctgagaagct cgacaaactg
     7681 ccgcgcgatc gctggctgga gctgggcctg acagacgaag agaaacaaaa tcagctggaa
     7741 cagctggctg agcagtatga cgaactgaaa cacgagttcg agaagaaact cgaagcgaaa
     7801 cgccgcaaaa tcacccaggg cgacgatctg gcaccgggcg tgctgaagat tgttaaggta
     7861 tatctggcgg ttaaacgccg tatccagcct ggtgacaaga tggcaggtcg tcacggtaac
     7921 aagggtgtaa tttctaagat caacccgatc gaagatatgc cttacgatga aaacggtacg
     7981 ccggtagaca tcgtactgaa cccgctgggc gtaccgtctc gtatgaacat cggtcagatc
     8041 ctcgaaaccc acctgggtat ggctgcgaaa ggtatcggcg acaagatcaa cgccatgctg
     8101 aaacagcagc aagaagtcgc gaaactgcgc gaattcatcc agcgtgcgta cgatctgggc
     8161 gctgacgttc gtcagaaagt tgacctgagt accttcagcg atgaagaagt tatgcgtctg
     8221 gctgaaaacc tgcgcaaagg tatgccaatc gcaacgccgg tgttcgacgg tgcgaaagaa
     8281 gcagaaatta aagagctgct gaaacttggc gacctgccga cttccggtca gatccgcctg
     8341 tacgatggtc gcactggtga acagttcgag cgtccggtaa ccgttggtta catgtacatg
     8401 ctgaaactga accacctggt cgacgacaag atgcacgcgc gttccaccgg ttcttacagc
     8461 ctggttactc agcagccgct gggtggtaag gcacagttcg gtggtcagcg tttcggggag
     8521 atggaagtgt gggcgctgga agcatacggc gcagcataca ccctgcagga aatgctcact
     8581 gttaagtctg atgacgtgaa cggtcgtacc aagatgtata aaaacattgt ggacggcaac
     8641 catcagatgg agccgggcat gccagaatcc ttcaacgtat tgttgaaaga gattcgttcg
     8701 ctgggtatca acatcgaact ggaagacgag taattctcgc tcaaacaggt cactgctgtc
     8761 ggggtaaaac ccggcagcgg attgtgctaa ctccgacggg agcaaatccg tgaaagattt
     8821 attaaagttt ctgaaagcgc agactaaaac cgaagagttt gatgcgatca aaattgctct
     8881 ggcttcgcca gacatgatcc gttcatggtc tttcggtgaa gttaaaaagc cggaaaccat
     8941 caactaccgt acgttcaaac cagaacgtga cggccttttc tgcgcccgta tctttgggcc
     9001 ggtaaaagat tacgagtgcc tgtgcggtaa gtacaagcgc ctgaaacacc gtggcgtcat
     9061 ctgtgagaag tgcggcgttg aagtgaccca gactaaagta cgccgtgagc gtatgggcca
     9121 catcgaactg gcttccccga ctgcgcacat ctggttcctg aaatcgctgc cgtcccgtat
     9181 cggtctgctg ctcgatatgc cgctgcgcga tatcgaacgc gtactgtact ttgaatccta
     9241 tgtggttatc gaaggcggta tgaccaacct ggaacgtcag cagatcctga ctgaagagca
     9301 gtatctggac gcgctggaag agttcggtga cgaattcgac gcgaagatgg gtgcggaagc
     9361 aatccaggct ctgctgaaga gcatggatct ggagcaagag tgcgaacagc tgcgtgaaga
     9421 gctgaacgaa accaactccg aaaccaagcg taaaaagctg accaagcgta tcaaactgct
     9481 ggaagcgttc gttcagtctg gtaacaaacc agagtggatg atcctgaccg ttctgccggt
     9541 actgccgcca gatctgcgtc cgctggttcc gctggatggt ggtcgtttcg cgacttctga
     9601 cctgaacgat ctgtatcgtc gcgtcattaa ccgtaacaac cgtctgaaac gtctgctgga
     9661 tctggctgcg ccggacatca tcgtacgtaa cgaaaaacgt atgctgcagg aagcggtaga
     9721 cgccctgctg gataacggtc gtcgcggtcg tgcgatcacc ggttctaaca agcgtcctct
     9781 gaaatctttg gccgacatga tcaaaggtaa acagggtcgt ttccgtcaga acctgctcgg
     9841 taagcgtgtt gactactccg gtcgttctgt aatcaccgta ggtccatacc tgcgtctgca
     9901 tcagtgcggt ctgccgaaga aaatggcgct ggagctgttc aaaccgttca tctacggcaa
     9961 gctggaactg cgtggtcttg ctaccaccat taaagctgcg aagaaaatgg ttgagcgcga
    10021 agaagctgtc gtttgggata tcctggacga agttatccgc gaacacccgg tactgctgaa
    10081 ccgtgcaccg actctgcacc gtctgggtat ccaggcattt gaaccggtac tgatcgaagg
    10141 taaagctatc cagctgcacc cgctggtttg tgcggcatat aacgccgact tcgatggtga
    10201 ccagatggct gttcacgtac cgctgacgct ggaagcccag ctggaagcgc gtgcgctgat
    10261 gatgtctacc aacaacatcc tgtccccggc gaacggcgaa ccaatcatcg ttccgtctca
    10321 ggacgttgta ctgggtctgt actacatgac ccgtgactgt gttaacgcca aaggcgaagg
    10381 catggtgctg actggcccga aagaagcaga acgtctgtat cgctctggtc tggcttctct
    10441 gcatgcgcgc gttaaagtgc gtatcaccga gtatgaaaaa gatgctaacg gtgaattagt
    10501 agcgaaaacc agcctgaaag acacgactgt tggccgtgcc attctgtgga tgattgtacc
    10561 gaaaggtctg ccttactcca tcgtcaacca ggcgctgggt aaaaaagcaa tctccaaaat
    10621 gctgaacacc tgctaccgca ttctcggtct gaaaccgacc gttatttttg cggaccagat
    10681 catgtacacc ggcttcgcct atgcagcgcg ttctggtgca tctgttggca tcgatgacat
    10741 ggtcatcccg gagaagaaac acgaaatcat ctccgaggca gaagcagaag ttgctgaaat
    10801 tcaggagcag ttccagtctg gtctggtaac tgcgggcgaa cgctacaaca aagttatcga
    10861 tatctgggct gcggcgaacg atcgtgtatc caaagcgatg atggataacc tgcaaactga
    10921 aaccgttatt aaccgtgacg gtcaggaaga gaagcaggtt tccttcaaca gcatctacat
    10981 gatggccgac tccggtgcgc gtggttctgc ggcacagatt cgtcagcttg ctggtatgcg
    11041 tggtctgatg gcaaagccgg atggctccat catcgaaacg ccaatcaccg cgaacttccg
    11101 tgaaggtctg aacgtactcc agtacttcat ctccacccac ggtgctcgta aaggtctggc
    11161 ggataccgca ctgaaaactg cgaactccgg ttacctgact cgtcgtctgg ttgacgtggc
    11221 gcaggacctg gtggttaccg aagacgattg tggtacccat gaaggtatca tgatgactcc
    11281 ggttatcgag gggggtgacg ttaaagagcc gctgcgcgat cgcgtactgg gtcgtgtaac
    11341 tgctgaagac gttctgaagc cgggtactgc tgatattctc gttccgcgca acacgctgct
    11401 gcacgaacag tggtgtgacc tgctggaaga gaactctgtc gacgcggtaa aagtacgttc
    11461 tgttgtatct tgtgacaccg actttggtgt atgtgcgcac tgctacggtc gtgacctggc
    11521 gcgtggccac atcatcaaca aaggtgaagc aatcggtgtt atcgcggcac agtccatcgg
    11581 tgaaccgggt acacagctga ccatgcgtac gttccacatc ggtggtgcgg catctcgtgc
    11641 ggctgctgaa tccagcatcc aggtgaaaaa caaaggtagc atcaagctca gcaacgtgaa
    11701 gtcggttgtg aactccagcg gtaaactggt tatcacttcc cgtaacaccg aactgaaact
    11761 gatcgacgaa ttcggtcgta ccaaagaaag ctacaaagtg ccttacggtg cggtactggc
    11821 gaaaggcgat ggcgaacagg ttgctggcgg cgaaaccgtt gcaaactggg acccgcacac
    11881 catgccggtt atcaccgaag taagcggttt tgtacgcttt actgacatga tcgacggcca
    11941 gaccattact cgtcagaccg acgaactgac cggtctgtct tcgctggtgg ttctggattc
    12001 cgcagaacgt accgcaggtg gtaaagatct gcgtccggca ctgaaaatcg ttgatgctca
    12061 gggtaacgac gttctgatcc caggtaccga tatgcctgcg cagtacttcc tgccgggtaa
    12121 agcgattgtt cagctggaag atggcgtaca gatcagctct ggtgacacct tggcgcgtat
    12181 tccgcaggaa tccggcggta ccaaggacat caccggtggt ctgccgcgcg ttgcggacct
    12241 gttcgaagct cgtcgtccga aagagccggc aatcctggct gaaatcagcg gtatcgtttc
    12301 cttcggtaaa gaaaccaaag gtaaacgtcg tctggttatc accccggtag acggtagcga
    12361 tccgtacgaa gagatgattc cgaaatggcg tcagctcaac gtgttcgaag gtgaacgtgt
    12421 agaacgtggt gacgtaattt ccgacggtcc ggaagcgccg cacgacattc tgcgtctgcg
    12481 tggtgttcat gctgtgactc gttacatcgt taacgaagta caggacgtat accgtctgca
    12541 gggcgtaaag attaacgata aacacatcga agttatcgtt cgtcagatgc tgcgtaaagc
    12601 taccatcgtt aacgcgggca gctccgactt cctggaaggc gaacaggttg aatactctcg
    12661 cgtcaagatc gcaaaccgcg aactggaagc gaacggcaaa gtgggtgcaa cttactcccg
    12721 cgatctgctg ggtatcacca aagcgtctct ggcaaccgag tccttcatct ccgcggcatc
    12781 gttccaggag accactcgcg tgctgaccga agcagccgtt gcgggcaaac gcgacgaact
    12841 gcgcggcctg aaagagaacg ttatcgtggg tcgtctgatc ccggcaggta ccggttacgc
    12901 gtaccaccag gatcgtatgc gtcgccgtgc tgcgggtgaa gctccggctg caccgcaggt
    12961 gactgcagaa gacgcatctg ccagcctggc agaactgctg aacgcaggtc tgggcggttc
    13021 tgataacgag taatcgttaa tccgcaaata acgtaaaaac ccgcttcggc gggttttttt
    13081 atgggtgtta ttcagagggc aaattcttca cggaaaatat taagaaaggc agggcgaaac
    13141 gaggaagaag cttttcgccc tggacaacat tcctgctgac ggcactacca taaaaaaaca
    13201 caacgctttc aacaaccttg cgtttttcat cttccgtagc ggctcggaat aaatttacgt
    13261 tcttacattc cgtctcatag tctttgcgag gcgcgtccca gatagctgtc ccagtctttc
    13321 cataccggct gcaaaccctg agcggttaac acggcagcaa ccgcttccgg tctgcgatcg
    13381 tcgtgcggtg agaactgttc caactcgggg tgattatcgg catagccacc tggctgcgtt
    13441 ttcgagaagg cgctgacgtt attgatcgcc agcggaataa cgcgatcgcg aaaccacggt
    13501 gattcccgcg tggagagtga cagttcaatc tccggtgcaa gcagtcggaa ggcgcagatg
    13561 gtttgcacta actggcgttc atccataatc gacgcaggct caatgccgcc agtacacggg
    13621 cgcaggcgcg gaaaggagac agagtaacgg ctttgccagt aatgctgttg cagccatagc
    13681 aaatgttcgg caaccatata gcagtcaacg cgccagttgt cggaaaggcc aattagcgcg
    13741 ccgaggccta tcttatcaat ccccgcacgc cccagccgat ccggcgtttc cagccgccag
    13801 aagaagtcct gttttttgcc tttcaaatga tggcgggtat aagtcgcctc gtgatatgtc
    13861 tcctgataaa ccatcacccc atccagacca agttgcttta actcggcgta ttccgtctcc
    13921 gccagcggtt gcacttccat ttgtagtgaa gagaactgtt cacgcagggc ggggagatga
    13981 cgacgaaagt aatccatccc cactttcgcc tgatgttcac cagtgactaa tagcagatgt
    14041 tcaaagccca tctcccgtat agcggcgctt tccctggcaa tatccgcttc atccagcgtt
    14101 ttgcgcttga tgcgattact catggaaaat ccgcagtacg tgcagtcgtt agcgcaaaga
    14161 ttggaaagat aaagcgggac gtagaaacta actgtgttgc caaatcgctg acgggtcaga
    14221 cgctgcgcct gttgggccag ttgttccaga tagccactgg cggcaggcga taacagcgcc
    14281 atcatgtcgt cgcgggtgag ttgcgaggca tttagcgccc gctctacgtc agcagccgtt
    14341 ttgccgttga tacgcaggcg gatgtcgtcc cagtccagtt gtcgccagcg attgctgaag
    14401 gttttcatgc cgatgcctcc agaaatccgg tcagcgggct ggtggcatga gcaaaataac
    14461 tgcggctgcc cggtccggac tgacgtgcca gcaggcctac ttctaccgcc agacgaaatg
    14521 ccttcgccat gttgacggga tcgtccgcga cggcaatcgc cgtattcact aacaccgcgt
    14581 cggcccccat ttccagcgcc tgcgtggcat ggctgggaac gccgatgcca gcatcgacaa
    14641 ccaccggaac tgtggcctgc tggataataa tctccagcat ggcacgggtt tccagtccct
    14701 gattcgagcc aatcggcgcg ccgagcggca tcaccgctgc acagccgact tcttccagac
    14761 gtttacacaa taccggatcg gccccgcagt aaggcagcac gacaaatccc tgttgtacca
    14821 gcgtttcggc ggctttcagg gtttcgatgg gatcgggcaa cagccagcgg gcgtcagggt
    14881 gaatctccaa ttttagccag tttgtgccta acgcttcacg tgccagatgg gcggcgaaaa
    14941 tggcttcttc cgctgttttc gccccggaag tattcggcag cagggccaca cccgccgcga
    15001 taagcggttc gaggatagcg tcgttgtgct ggcgcaagtc gacacgtttc atcgccagtg
    15061 tcaccagctg gctgccggaa gcgcggatcg cctccaccat cagttgtgaa gaagcgaatt
    15121 tccctgtgcc ggtaaacaga tgtgaatcaa acgttttgtc cgcaatacgt aacatctcaa
    15181 ccccctgcaa taacctgaaa aagcaggatc tggtcgccat cctgcacgat atgttgcgtc
    15241 cactgctcac gcgggacgat ttgctgatta atcgccagag ccgcgcccgc ttgtcgttgg
    15301 tcgagttgct ccagtagttc gtgaacagtt tgcccggcgg cgcactgcat cggttgatcg
    15361 ttaaacagga tctgcattgc ttcctccgca taccgggcaa ccactggcgc ggcgcaacgc
    15421 caggctgcgc cactggctcg acttaccgtc gaacagtcgg agttctcccg caggcgtctc
    15481 tataccgctt aataacttaa tggcttccag tgcctgcaaa gtgcccatca ccccgaccac
    15541 cgggccaacc acgcccgccg tgcggcagtt gcgttctggc tgctggttat ctggccacag
    15601 gcagcggtaa cacccctgct cccagggcgg tgtcagtacc atcaactgac cgccaaatcc
    15661 gaccgcgctg gcggtgataa gcggcgtgtt gagtgccacg caggcggcat taatctcctg
    15721 gcgagtcgcc atattgtcgg tacagtcgag caccacatcg gcttgtgcaa ccgcatcttt
    15781 caacgcctca cccgttaacc gttgttgtaa tgctgtcagt tgaatgtcgg gattcaactg
    15841 tgtcagtcgc tgttggctga cctgcgattt cgggctgttg atatcttcag tggtaaagag
    15901 gatttgtcgt tgcagattgc ttaaatgcac atcgtcgtca tctgccagta ccagcgtccc
    15961 gacgccagcc cctgccaggt atagcgcagc aggtgtaccc agcccgccaa gaccaataat
    16021 cagcacctgg ctgtcgagca gtttttgctg cccgtcaaga gcgatatcgt cgagcagaat
    16081 ttggcggcta taacgcataa agtcacggtc attcatcgcc aactcctgca atttccagca
    16141 actgtgccgt tgccaaacgc cagtctgcgg cttgagtaat ggcgctgacg acggcgatac
    16201 tgccgacacc cgttgctatc accgcaggcg cgcgtgccag actgataccg ccaatcgcca
    16261 cggtgggata atccgccagg cgctcaacat gccgtgccaa ctgttccagc ccctgcggtg
    16321 cagaaggcat ctgtttggtt tgcgtcggga acacatgtcc cagcgcgata taagaggggc
    16381 gtgctgccag cgcgacgtcg atttccatat cgtcatgtgt cgaaacgccc agccgcaggc
    16441 ctgccgcgcg gatggcattg agatcggtgg cttgcaaatc ttcctgcccc aaatggacgc
    16501 catacgcctg atgcttgatc gcgagccgcc agtaatcgtt gataaacaat cgcgcgttat
    16561 agcggcggcc cagcgcaatt gccgccacga catcggcttc cacctcttca tcgcgccgat
    16621 ctttgatgcg tagctggaga gtacgtacgc ctgcatccaa cagacgttcg atccactgta
    16681 cgctgtccac caccgggtac agtcctaaac gaaaaggtac aggaggaaaa tcaggctgat
    16741 acatcacgct tcctccttat gcaggtagat ttctccgcct ctggcacgga agttctccga
    16801 catatccgcc attcccactt caatggtttg cgcggcggcg taatcgcgta cttcctggct
    16861 gattttcatc gagcagaatt ttggcccaca catggagcaa aaatgggcga ctttgccgga
    16921 ctcttggggc agggtttcat cgtgataagc gcgggcggta aacgggtcga gggccagatt
    16981 aaactggtct tcccagcgaa attcgaagcg ggctttcgac atggcgttat cgcgaatttg
    17041 cgcgcccgga tgcccttttg ccaggtcagc agcgtgggcg gcaatcttat aagtgataag
    17101 cccctgctta acatcttctt tattgggcag acccagatgc tcttttggcg ttacgtaaca
    17161 gagcatcgcg cagccaaacc agccaatcat cgccgcacca atccccgacg tgaagtggtc
    17221 atagcccggt gcaatatcgg tagttagcgg ccccagagtg taaaacggcg cttcgtggca
    17281 gtgctctaac tcctcggtca tattgcggcg gatcatctgc atcggcacat ggcctgggcc
    17341 ttcaatcatc acctgtacgt catattccca ggcgattttg gtcagttcgc ccagcgtatg
    17401 caattcggca aactgcgctt catcgttggc gtcctgaata gaaccggggc gcagaccgtc
    17461 gcccagcgac agcgaaacgt cataagcggc acagatttca caaatttcgc ggaagtgttg
    17521 atagaggaaa ttttcctgat gatgggagag gcaccatttc gccataatcg aaccgccgcg
    17581 agagacgata ccggtcaggc gtttcgcggt catcggcaca tagcgcagca gtacgcccgc
    17641 atggatagtg aagtaatcca caccttgctc ggcctgttcc agcagcgtgt cgcggaacgc
    17701 ttcccaggta agatcttcgg cgatcccgtt aaccttctcc agcgcctggt agatcggcac
    17761 tgtaccgatc ggcaccggac tgttacgcaa aatccactcg cgggtttcgt gaatatagcg
    17821 accggtggag agatccatca ccgtatccgc tccccagcgc gtggaccata ccagcttttc
    17881 cacttcttct tcgatggaag aggtgaccgc cgagttgccg atattggcgt taacttttac
    17941 caggaagtta cggccaataa tcatcggctc cgattccgga tgattaatgt tggccgggat
    18001 aatcgcacgt ccggcagcaa cttcatcacg gacaaattcc gcagtgatat tttccggcag
    18061 atgtgcgcca aagctcattc ccggatgctg gtggcgtaaa acctcgctac ggatgcgctc
    18121 gcggcccata ttctcgcgga tggcgatgaa ttccatttcc ggcgtgatga tgccctggcg
    18181 ggcgtagtgc agttgggtga cacggcgtcc tgttttggcg cgttttggcg ttaataagcc
    18241 gctaaaacgt agctcgtcga gaccatcatc tgccagccgc gttctggtgt aatcggaact
    18301 gcgcacggta agttcttcgg tatcggcgcg cgcatcgatc cacggctggc gtagttttgc
    18361 cagcccttgc tgcacgttaa tggcgatctg cggatcacca tacgggccgg aggtgtcgta
    18421 gaccggaatc gcttcgtttt cttcgtactg cggctgttct ttgctaccgc caattagcgt
    18481 cgggctaagc tggatctcac gcatcggcac gcgcacgccg gggtgtgtgc cagtgatata
    18541 aatgcgtttt gagttgggaa aggcggtgcc ttccagggtg tcgataaaat gttgggcccg
    18601 ggcgcgttgt tcgcggcggg tcagttttgt tgcagacata gctcattcca aaaagttaag
    18661 gacgtggctt gtcagacgac ggatgaagca agagacgatc gccgcaaagg cgatgcgata
    18721 gcagattaac tcttgttccc ttcgcaggta ttagcctgat caggttccgc ggatcccgaa
    18781 taaacggtct cagccagtta aggcactccg acaagaaatt agcccccaca aacggggcgt
    18841 tgaatgtaaa ttacgcgtta acagcgcaga actcaagcag gatgtttgac gcgggcggcg
    18901 tcaagcacca gcagaatcag cttatcttcc agcacaaagc gcgcttccag cgcctcgccg
    18961 atgtcagata aaacctgttg aaactcaagg taattatcat gatcgatggc ggtttccagg
    19021 ctggaatcgt agtaatccat aatctgttgg gtattggctt cgagttgcgg ccaaatcttc
    19081 gcggcgcgcg cgagttgccc gttgccttcc agcttatgaa gaatgcgctc ataaatactg
    19141 aaatgtccgg cagacaagta atcgaccagg ctctgacaaa aatcatcaag ggctttttcg
    19201 tttagcctca tgtacgattc tttgccaggc ttaatgccaa ccagattgta gtaagccacg
    19261 agcagatgct tacgtacatg tagccagcga tcaaccagtt tgttacttcc tctgacgcgc
    19321 tccgtcaggt tatcgagctg gttaagcatg attgactccg caagtttgta ttcaaaaact
    19381 gctcagtgag aaatgtaaaa accatgttaa acatgccagt gatgcaaagg tagtgcaaga
    19441 gctatggatc gtataattga aaaattagat cacggctggt gggtcgtcag ccatgaacaa
    19501 aaattatggt tgccgaaggg agaattgcca tatggcgaag cggcaaattt cgatcttgtg
    19561 ggtcagcgcg cactacaaat cggcgaatgg cagggggaac ctgtttggtt aatacaacag
    19621 cagcggcgtc acgatatggg gtcggtacgt caggtcattg atctcgatgt tgggctgttt
    19681 caactggccg gacgtggcgt gcaactggcg gagttttacc gatcgcataa atactgtggt
    19741 tactgcgggc atgaaatgta tccgagcaaa accgaatggg cgatgctgtg tagccattgc
    19801 cgtgagcgtt actacccgca aatcgccccc tgcattattg ttgccatccg tcgcgatgat
    19861 tcgctcctcc tcgcccagca tacccgccat cgtaacggtg tccatacagt acttgccgga
    19921 ttcgtcgaag tgggcgaaac cctcgagcag gcagtcgcgc gggaagtgat ggaagagagc
    19981 ggaattaaag ttaaaaactt gcgttacgtg acttctcagc cgtggccgtt tcctcagtct
    20041 ttaatgaccg cgtttatggc ggaatatgac agcggcgaca tcgtgatcga cccgaaagaa
    20101 ttgctcgagg cgaactggta tcgctatgac gatttgccgt tactcccgcc gcccggcacc
    20161 gtagcgcgcc gtctgataga agatactgtg gcgatgtgtc gggcagagta tgagtgatga
    20221 tacactgacc gcctgacgca ctaaggaaca gccaaaatga ccgaacttaa aaacgatcgt
    20281 tatctgcggg cgctgctgcg ccagcccgtt gatgtcactc cagtatggat gatgcgccag
    20341 gcgggtcgct atctaccgga atataaagcc acgcgcgccc aggcgggcga ttttatgtcg
    20401 ctgtgcaaaa acgccgagct ggcgtgcgaa gtgactttgc aaccgctgcg tcgctacccg
    20461 ctggatgcgg cgatcctctt ttccgatatc ctcaccgtgc cggacgcgat ggggttaggg
    20521 ctctattttg aagccggaga aggtccgcgt tttacctcgc cagtcacctg caaagccgac
    20581 gtcgataaac tgccaattcc ggacccggaa gatgagctgg gttacgtgat gaacgcggtg
    20641 cgtaccattc gtcacgaact gaaaggcgaa gtgccgctga ttggtttttc cggcagcccg
    20701 tggacgctgg caacctacat ggtggaaggc ggcagcagca aagccttcac cgtgatcaaa
    20761 aaaatgatgt atgccgatcc gcaggcgctg cacgctctgc tcgataaact ggcgaaaagc
    20821 gtcactttgt atctgaatgc gcagattaaa gccggtgctc aggcagtgat gattttcgac
    20881 acctggggcg gcgtgcttac cgggcgcgat tatcaacagt tctcgctcta ttacatgcat
    20941 aaaattgttg atggtttact gcgtgaaaac gacggtcgcc gcgtgccagt cacgctgttt
    21001 accaaaggcg gcggacagtg gctggaagcc atggctgaaa ccggttgcga tgcgctgggc
    21061 ctcgactgga caacggacat cgccgatgcg cgccgccgtg tgggcaataa agtcgcgttg
    21121 cagggtaata tggatccgtc gatgctgtac gcgccgcctg cccgcattga agaagaagta
    21181 gcgactatac ttgcaggttt cggtcacggc gaaggtcatg tctttaacct tggtcacggc
    21241 attcatcagg atgtgccgcc agaacatgct ggcgtgttcg tggaggcagt gcatcgactg
    21301 tctgaacagt atcaccgcta agagcctgat ccatcaggct attttatttg ccattttgag
    21361 gccgggcagt gctcaaaatc ctcacgtact acgtgtacgc tccggttttt ccgcgctgtc
    21421 cggcctcaaa ctgccttcaa caattacgcc tgttgaacca ggctctaagg agcgattatg
    21481 gatctcgcgt cattacgcgc tcaacaaatc gaactggctt cttctgtgat ctgcgaggat
    21541 cgactcgata aagatccacc ggatctgatc gccggagccg atgtcgggtt tgagcagggc
    21601 ggagaagtga cgcgagcggc gatggtgctg ctgaaatatc cctcgcttga gctggtggag
    21661 tataaagttg cccgcatcgc caccaccatg ccttacattc caggttttct ttccttccgc
    21721 gaatatcctg cgctgctggc agcgtgggag atgctgtcgc aaaagccgga tttagtgttt
    21781 gtcgatggtc atgggatctc gcatcctcgc cgtcttggcg tcgccagcca ttttggctta
    21841 ttggtggatg tgccgaccat tggcgtggcg aaaaaacggc tctgcggtaa attcgaaccg
    21901 ctctccagca aaccgggcgc gctggcccca ctgatggata aaggcgagca gctggcctgg
    21961 gtctggcgca gcaaagcgcg ctgtaacccg ttgtttatcg ctaccggcca tcgggtcagc
    22021 gtggacagcg cgctggcgtg ggtacaacgc tgcatgaaag gctatcgtct gccggagcca
    22081 acgcgctggg cggacgcggt ggcctcggaa cgtccggcgt tcgtgcgcta tacagcaaat
    22141 cagccctaat tcaggtaaac tgcggataat ttccgtattt gagaactcaa catgttacaa
    22201 aacccaattc atctgcgtct ggagcgcctg gaaagctggc agcatgtcac tttcatggct
    22261 tgcttatgcg aacgcatgta ccccaattac gccatgttct gccagcaaac cggttttggt
    22321 gatgggcaaa tttaccgtcg tattctcgat ctcatctggg aaacgctgac cgttaaagac
    22381 gcaaaagtaa atttcgacag ccaactggag aaatttgaag aagcgattcc ttcagccgac
    22441 gatttcgatc tgtacggcgt ttatccggca atcgatgcct gcgtggcgtt aagtgaactg
    22501 gtccattcgc gtttgagtgg tgaaacgctc gaacacgcgg tggaagtgag taagacctcc
    22561 atcacgaccg ttgcgatgct ggaaatgact caggctggtc gcgaaatggg cgatgaagag
    22621 cttaaagaaa acccagctgt agagcaagaa tgggacattc agtgggaaat attccgactt
    22681 ttagctgagt gcgaagaacg cgatatcgag ctgataaaag gccttagggc agacctgcgt
    22741 gagtcgggtg agagcaatat tggtataatt tttcagcaat aagaccagaa aacgtgattt
    22801 aacgcctgat ttgtcgtacc tggagtcttc cctttcgccc cccgtctggt ctacatttgg
    22861 ggggcgaaaa aaagtggcta tcggtgcgtg tatgcaggag agtgctattc tggcatttcc
    22921 gtcgcactcg atgcttagca agcgataaac acattgtaag gataacttat gaacaagact
    22981 caactgattg atgtaattgc agagaaagca gaactgtcca aaacccaggc taaagctgct
    23041 ctggagtcca ctctggctgc aattactgag tctctgaaag aaggcgatgc tgtacaactg
    23101 gttggtttcg gtaccttcaa agtgaaccat cgcgctgagc gtactggccg caacccgcag
    23161 accggtaaag aaatcaaaat tgccgcagct aacgtaccgg catttgtttc tggcaaggca
    23221 ctgaaagacg cagttaagta agattgcgtg gcagtgaaca gttttaacga aggggtggtt
    23281 tcaccccttt tgtctttctg gcgtcgatca ttgatgctgg caggtgcgct gtttctcact
    23341 gcctgtagcc acaactcttc acttcctccc tttaccgcca gtggatttgc tgaagaccag
    23401 ggcgcggtac gcatctggcg aaaagacagc ggcgataatg tgcatctgct tgccgtgttt
    23461 agcccgtggc gcagtggcga taccacgacg cgagagtatc gctggcaggg cgataacctc
    23521 acgctcatca atatcaatgt ttacagcaaa ccgccggtga acattcgcgc gcgttttgac
    23581 gatcgcggcg atctgagctt tatgcaacgt gaatccgatg gggaaaagca gcagctttct
    23641 aacgaccaaa tcgatttata ccgttatcgt gctgatcaga tccgccagat tagcgatgcc
    23701 ttacgtcagg ggagagtcgt gctgcgccag gggcgctggc atgcgatgga acagaccgtg
    23761 accacctgcg aagggcaaac cattaaacct gatttagatt cgcaggcgat agcgcatatc
    23821 gagcgccgcc agagccgctc ttctgttgat gtcagcgtgg catggctgga agcgcccgaa
    23881 ggttcgcaat tactgttagt ggcaaactct gatttctgtc gctggcaacc caacgagaaa
    23941 acgttctgat ttaccagtgg cccataccca tatgaccgcc accaccgcag ccgccgtagc
    24001 ccatgcccat tccggctccg cgcggaatac ccgcttcagc catcgcgata tctcgtttca
    24061 cccgtaactc atctaacgac tgacgcaaat tctccatctc tttggcgacc gcgttaattt
    24121 tgctgctatc cggtgggttc gcggctaaca gggcattgta ttcataacgc ttcgtcacca
    24181 gttgctgttg cagtgcgctg ctttgagcgt aaaagtcatt atggattttc tgccacgctg
    24241 tctgttgttc gctggtcaaa ggcgcggcat tttgctgcca cataccgtgt ccgccgtgag
    24301 caaatgcaga tgtcgatccc atcgccattg ctgaaagcgc catcattacc agggcaattt
    24361 tcgtgttccg tttcatggtt aatcctccag tggttgtctt acttcgggta ttgcatcttc
    24421 cgtgccaacg atgaaacgct gatatgacgg gtaatctggc atgataaacg agtaaaaatg
    24481 actcgcctgc tgcgggtagc gagtcatttt tactcattga aacttaagcc tttgtgttac
    24541 agcgcagggt aagcgctgat aaaagatggc atgatttctg ctgtcagaaa gggatgagca
    24601 ggcaaagaag aagatgcgtt ttatgcaacg ttctaaagac tccttagcta aatggttaag
    24661 cgcgatcctc cccgtggtca ttgttgggct ggtagggttg tttgcggtga cggtgattcg
    24721 cgattatggg cgcgagactg ccgccgccag acaaacgctg ctggaaaaag gcagtgtact
    24781 tattcgcgct cttgaatccg gctcgcgcgt cggcatgggg atgcgcatgc atcatgcgca
    24841 gcagcaggcc ttactggaag aaatggccgg gcagcctgga gtacgttggt ttgcggtcac
    24901 ggatgaacaa ggaacaatcg tgatgcatag caactccggc atggtgggaa aacagcttta
    24961 ttcccctcag gaaatgcagc agttacatcc gggagatgaa gaagcgtggc ggcggatcga
    25021 tagcgcagac ggcgagcctg ttctggaaat ttatcgccag tttcaaccga tgtttgctgc
    25081 tggaatgtac cggatgcgcc atatgcagca gtatgccgcg acaccacaag caattttcat
    25141 cgctttcgac gccagtaata ttgtgagtgc cgaagatcgt gagcagagaa acaccctgat
    25201 tattctcttc gccctggcga cggtcttgct ggcaagcgta ttgtcattct tctggtatcg
    25261 ccgctatctg cgctcgcgcc agcttctaca agatgaaatg aagcgcaaag agaagctggt
    25321 ggcgctgggg catcttgcgg caggcgttgc ccacgaaatc cgtaacccac tttcctcgat
    25381 taaaggactg gcgaaatact ttgccgagcg cgcgcctgca gggggagaag cgcatcaact
    25441 ggcgcaggtg atggcgaaag aggccgaccg tttaaaccgc gtggtaagcg agttgctgga
    25501 actggttaag ccaacgcatc tggctttgca ggcggtggat ctcaacacgc tgattaacca
    25561 ctcattacag ctggtaagcc aggatgcaaa cagccgggag atccagttac gctttaccgc
    25621 caacgacaca ttaccggaaa ttcaggccga tccggacagg ctgactcagg tcctgttgaa
    25681 tctctatctc aatgctattc aggcgattgg tcagcatggc gtgattagcg tgacggccag
    25741 cgaaagcggc gcgggcgtga aaatcagcgt taccgacagc ggtaagggaa ttgcggcaga
    25801 tcagcttgaa gccatcttca ctccgtactt caccaccaaa gccgaaggca ccggattggg
    25861 gctggcggtc gtgcataata ttgttgaaca acacggtggt acaattcagg tcgcaagcca
    25921 ggagggaaaa ggctcaacgt tcaccctctg gcttccggtc aatattacgc gtaaggaccc
    25981 acaaggatga cgcacgataa tatcgatatt ctggtggtgg atgatgacat tagccactgc
    26041 actattttgc aggctttact gcgcggctgg ggctataacg tcgcgctggc gaacagcggg
    26101 cgacaggcgc tggagcaggt gcgggaacag gtttttgatc ttgtgctttg cgatgtgcga
    26161 atggcggaga tggacggcat cgccacgctg aaagagatca aagcgttaaa cccggcaatt
    26221 ccggtgctga ttatgactgc gtactccagc gtcgagacgg cggtagaggc actgaaaact
    26281 ggggcgcagg attatctcat caagccgctg gatttcgata acctacaggc gacgctggaa
    26341 aaggcgctcg cgcatacgca cagtattgat gctgaaacac ctgcggtgac tgccagccag
    26401 ttcggtatgg tcggtaaaag cccggcgatg caacacctgc tcagtgaaat cgccctcgtc
    26461 gcgccatcgg aagccacggt gctgatccac ggcgattccg gcaccggtaa agagctggtc
    26521 gccagggcga ttcacgccag tagcgcacgt agcgaaaaac cgctggtaac gctcaactgt
    26581 gcggcactca acgaatcctt gctggaatct gaattgttcg gtcacgaaaa aggggcgttt
    26641 actggagccg ataaacgccg ggaagggcgc tttgttgagg cggacggcgg cacgctgttt
    26701 ctcgatgaaa ttggcgatat ctcgccgatg atgcaggtgc gtctgctgcg tgcgattcag
    26761 gagcgcgaag ttcagcgtgt cggtagcaac cagactatct cggttgatgt ccggctgatt
    26821 gcggcgaccc atcgcgatct tgccgcagag gtgaatgccg ggcgttttcg ccaggatctc
    26881 tactatcgcc tgaatgtggt ggcgattgaa gtaccatcgc tgcggcaacg gcgggaagat
    26941 atccctctgc tggctggcca ttttcttcag cgctttgccg agcgtaatcg caaggcggta
    27001 aaaggcttta cgccacaggc gatggatttg ctgatccact acgactggcc gggaaatatt
    27061 cgtgaactgg aaaacgcggt ggaacgggcg gtggtgctgc tgaccgggga atatatttcc
    27121 gaacgcgagc tgccgctggc gattgccagt acgccgatcc cgctggggca aagtcaggat
    27181 attcagccgc tggtggaagt ggaaaaagag gtgattctgg cggcgctgga gaaaacgggc
    27241 ggcaacaaaa ccgaagccgc ccgtcagtta gggatcacgc gcaaaacgct attggcaaaa
    27301 ctgtcgcgtt agttctgctc gcgttcgatg gcgcgccaac cgatatcttt ccggcagaag
    27361 caatcatccc agtggatatc ggtcattaag gcgtaggcgc gtttctgcgc ttctgccacg
    27421 gtatgaccca gcgcggtgac gcacagtacg cgcccgccgc tggttaccac ctgctcgtca
    27481 tccgccagtt ttgtgcccgc gtggaacact ttgccgtcag ccacttcttc cagcggcagg
    27541 ccgtggatca catcgccggt gcggtaatcg cccggatatc cacccgcagc catcaccacg
    27601 ctgagagaag cacgttcatc ccactctgac gttttctcgt ccagtttgcc ttcacaggcc
    27661 gccaggcaga gctcaaccag atcggacttc atgcgcagca taatcggctg ggtttccgga
    27721 tcgccaaagc ggcagttaaa ttcaataacc ttcggattgc cctgtttgtc gatcatcagg
    27781 cccgcataga gaaaaccggt gtaggtgttg ccttccgccg ccatgccttt cacggttggc
    27841 cagatgatac gttccatggt gcgctgatga acttcatcgg ttactaccgg cgcgggggag
    27901 taagcgccca tcccgccggt gtttggtccg ttatctttat cgcctacgcg tttgtgatcc
    27961 tggctggtag ccatcggcag cacatgctcg ccgtccacca tcacgataaa gctcgcttct
    28021 tcgccatcga ggaactcttc gataacgatg cgatgacccg cgtcgccaaa agcgttgccc
    28081 gccagcatat cgtgaacagc cgcttccgct tcttccagcg tcatcgccac gataacgcct
    28141 ttcccggcag ccagaccgtc cgctttaatg acgattggcg cgcctttctc acgcagatac
    28201 accagcgcag gttctacctc ggtgaagttc tggtattccg ccgtagggat cttatggcgg
    28261 gccaggaaat ctttggtaaa cgcttttgag ccttccagtt gggccgcacc tgcggttggg
    28321 ccgaagattt tcagcccggc ggcgcggaag gtatcgacca cgcctttcac cagcggcgct
    28381 tccgggccga cgatggtcag atcaatcttt tcgttttgtg cgaaatccaa cagcgccggg
    28441 atatcggtca cgccgatagc aacgttttgc agcgcgggtt ccagcgcagt gcctgcatta
    28501 cccggagcag caaaaacagt ctcaaccagc ggcgactggg ccgctttcca ggccagcgcg
    28561 tgctcgcgcc cgccgttacc aatcactaat actttcatct attgctccat taatggcgga
    28621 agtggcgcat gtcggtgaag agcatcgcaa taccgtgctc gtcggcggcg gcaatcactt
    28681 cgtcatcacg gatggaaccg ccaggctgga ttacgcaggt cacgcccgca gcggcggcgg
    28741 catcaatacc gtcgcggaac gggaagaacg cgtcagaagc catcgaggaa cctttcactt
    28801 ccaggccttc atcggcagct ttaataccgg cgattttcgc ggagtacacg cggctcatct
    28861 ggcccgcgcc aataccgatg gtcatattgt ttttggcata gacgatagca ttggatttca
    28921 cgaatttcgc cactttccag cagaacagcg catcacgcag ttcctgttcg gtcggctgac
    28981 gttgggtgac gacgcgcagt tcttccgcac cgaccatccc caggtcacga tcctgaacca
    29041 gcagaccgcc gttcacgcgt ttgaaatcaa gacccggaac acgctcgccc cactgaccgc
    29101 aggtcagaac gcgtacgttc tgcttggcgg cggtgatttt cagggcttct tcgctggcgg
    29161 aaggcgcaat aatcacttcg acaaactgac gagaaatgat ggcctgtgcg gtttccgcat
    29221 ccagctcgcg gttaaaggca atgatgccgc cgaatgcgga ggttgggtcg gttttgtacg
    29281 cgcgatcgta agcatcaaga atggaattgc cgatagctac accgcaaggg ttggcgtgct
    29341 tcacaatcac acatgccggc tcggcgaact ctttcacgca ctccagcgcc gcatcggtat
    29401 cggcgatgtt gttataagag agggctttac cctgaacctg ggttgcggta gcaacggagg
    29461 cttctttcac attctcttct atatagaagg cagcctgctg gtggctgttc tcgccgtaac
    29521 gcatatcctg cttcttaatg aagttcaggt tcagcgtgcg tgggaagcga ccggcggctt
    29581 ctttgctttc accgtggtac gccggaacca tgctgccgaa gtagttggca atcatgctgt
    29641 cgtaggcggc agtgtgttcg aaggctttga tggcgaggtc gaaacgggtt gcaagcgtca
    29701 gcgatccttc gttgtcatcc atctctttaa taatggcgtc gtagtcgctg ctcttcacca
    29761 cgattgcgac atctttatgg ttcttagcgg cggagcgcac catggtcggg ccgccgatat
    29821 cgatgttctc aaccgcatct tccagcgagc agccttcacg agcaacagtc tgggcgaacg
    29881 gatacaggtt aacaaccacc atatcgatag gctggatctg atgttcttcc ataatggcat
    29941 cgtcctggcc gcgacggccc agaatgccac catgtacttt cggatgcagg gtcttcacgc
    30001 gtccatccat catctccggg aaaccggtgt aatcggaaac ttcggttacc ggcagacctt
    30061 tttctgctaa cagacgggca gtgccccctg tagacagcag ctccacaccg cgtgcggaaa
    30121 gtgcctgggc gaattcgacg ataccggctt tgtcagaaac actgagcaga gcgcggcgga
    30181 ctggacgacg ttgttgcatg gtaaatcccc tgggtttgac tattacagag agcgttagct
    30241 gaatttttcg cgaaaaactc agctaacgcc cctaacgggg catccttatt tttcgcccgc
    30301 attgtaacga aaacgtttgc gcaacgctcg cgaatttttc tctttcaatg gtgatcacaa
    30361 ttttgacagt ggttaccgtg ggaaaaatac agaaattaca ttgatgattg tggataactc
    30421 tgtgcgtaaa aaggtataaa gcggggtttt gctggggaat gcagcagtca gtcatttttc
    30481 tgcaattttt ctattgcggc ctgcggagaa ctccctataa tgcgccttca tcgacacggc
    30541 ggatgtgaat cacttcacac aaacagccgg ttcggttgaa gagaaaaatc ctgaaattca
    30601 gggttgactc tgaaagagga aagcgtaata tacgccacct cgcgacagtg cgctaaagcg
    30661 cgtcgc
//
LOCUS       DAEBXN010000040        30298 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.040, whole genome shotgun sequence.
ACCESSION   DAEBXN010000040 DAEBXN010000000
VERSION     DAEBXN010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30298
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.040"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(<1..817)
                     /gene="wcaD"
                     /locus_tag="KJL99_001891"
     CDS             complement(<1..817)
                     /gene="wcaD"
                     /locus_tag="KJL99_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HBD4436638.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISIKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYILIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDALILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFRVGVVIAFPYISTRLGDLG"
     gene            complement(792..2009)
                     /gene="wcaC"
                     /locus_tag="KJL99_001892"
     CDS             complement(792..2009)
                     /gene="wcaC"
                     /locus_tag="KJL99_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HBD4436639.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLNSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(2006..2494)
                     /gene="wcaB"
                     /locus_tag="KJL99_001893"
     CDS             complement(2006..2494)
                     /gene="wcaB"
                     /locus_tag="KJL99_001893"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HBD4436640.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(2497..3336)
                     /gene="wcaA"
                     /locus_tag="KJL99_001894"
                     /pseudo
     CDS             complement(2497..3336)
                     /gene="wcaA"
                     /locus_tag="KJL99_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
     gene            complement(3429..5591)
                     /gene="wzc"
                     /locus_tag="KJL99_001895"
     CDS             complement(3429..5591)
                     /gene="wzc"
                     /locus_tag="KJL99_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HBD4436641.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITVVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKNQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIIDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(5594..6037)
                     /gene="wzb"
                     /locus_tag="KJL99_001896"
     CDS             complement(5594..6037)
                     /gene="wzb"
                     /locus_tag="KJL99_001896"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HBD4436642.1"
                     /translation="MFNNILVVCVGNICRSPMAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMDKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYHKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(6043..7182)
                     /gene="wza"
                     /locus_tag="KJL99_001897"
     CDS             complement(6043..7182)
                     /gene="wza"
                     /locus_tag="KJL99_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HBD4436643.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALANAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            7491..7640
                     /locus_tag="KJL99_001898"
     CDS             7491..7640
                     /locus_tag="KJL99_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4436644.1"
                     /translation="MVIEYWLANNAGGSVTANGIAGKVTIRQLAIFKNYIKYHSIWFL
                     GVSLG"
     gene            7841..9424
                     /locus_tag="KJL99_001899"
     CDS             7841..9424
                     /locus_tag="KJL99_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HBD4436645.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAKTASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(9876..11729)
                     /gene="asmA"
                     /locus_tag="KJL99_001900"
     CDS             complement(9876..11729)
                     /gene="asmA"
                     /locus_tag="KJL99_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HBD4436646.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQSQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(11751..12332)
                     /gene="dcd"
                     /locus_tag="KJL99_001901"
     CDS             complement(11751..12332)
                     /gene="dcd"
                     /locus_tag="KJL99_001901"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HBD4436647.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(12424..13065)
                     /gene="udk"
                     /locus_tag="KJL99_001902"
     CDS             complement(12424..13065)
                     /gene="udk"
                     /locus_tag="KJL99_001902"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HBD4436648.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            13383..13625
                     /locus_tag="KJL99_001903"
                     /pseudo
     CDS             13383..13625
                     /locus_tag="KJL99_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
     gene            13624..14565
                     /gene="mdtA"
                     /locus_tag="KJL99_001904"
                     /pseudo
     CDS             13624..14565
                     /gene="mdtA"
                     /locus_tag="KJL99_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
     gene            14565..17687
                     /gene="mdtB"
                     /locus_tag="KJL99_001905"
                     /pseudo
     CDS             14565..17687
                     /gene="mdtB"
                     /locus_tag="KJL99_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
     gene            17688..20753
                     /gene="mdtC"
                     /locus_tag="KJL99_001906"
     CDS             17688..20753
                     /gene="mdtC"
                     /locus_tag="KJL99_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HBD4436649.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLTPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNISIPKTFFPEQDTGVLMGGIQADQSI
                     SFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQQIID
                     RLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRKKLAT
                     LPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIYQPMN
                     QYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGLSAAS
                     TISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILIIAAI
                     ATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLIGIVK
                     KNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSGGDGS
                     ELRQPLEITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            20754..22169
                     /locus_tag="KJL99_001907"
     CDS             20754..22169
                     /locus_tag="KJL99_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD4436650.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHISVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            22166..23569
                     /gene="baeS"
                     /locus_tag="KJL99_001908"
     CDS             22166..23569
                     /gene="baeS"
                     /locus_tag="KJL99_001908"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HBD4436651.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            23566..24288
                     /gene="baeR"
                     /locus_tag="KJL99_001909"
     CDS             23566..24288
                     /gene="baeR"
                     /locus_tag="KJL99_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HBD4436652.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDVPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            24479..24811
                     /locus_tag="KJL99_001910"
     CDS             24479..24811
                     /locus_tag="KJL99_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HBD4436653.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            24958..26319
                     /gene="yegQ"
                     /locus_tag="KJL99_001911"
     CDS             24958..26319
                     /gene="yegQ"
                     /locus_tag="KJL99_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HBD4436654.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIHNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(26661..26978)
                     /locus_tag="KJL99_001912"
     CDS             complement(26661..26978)
                     /locus_tag="KJL99_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4436655.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            27384..28283
                     /gene="yegS"
                     /locus_tag="KJL99_001913"
     CDS             27384..28283
                     /gene="yegS"
                     /locus_tag="KJL99_001913"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HBD4436656.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(28365..29138)
                     /locus_tag="KJL99_001914"
     CDS             complement(28365..29138)
                     /locus_tag="KJL99_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBD4436657.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIVTDDAVSEVDKLALQKTRVKLITV"
     gene            complement(29244..30284)
                     /gene="gatD"
                     /locus_tag="KJL99_001915"
     CDS             complement(29244..30284)
                     /gene="gatD"
                     /locus_tag="KJL99_001915"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBD4436658.1"
                     /translation="MKSVVNDTDGIVRVAKSVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMQGKVLLIP"
BASE COUNT         7012 a         8046 c         7829 g         7411 t
ORIGIN      
        1 tacctaaatc gcccagacgg gtggaaatat aaggaaacgc gattaccaca ccaacccgga
       61 atacagccag agaaatcaat gctaacggta attttttctt aatcgcctct ttattcagat
      121 attgaaatgc ccactccagc aaataaaaca ggataaaggt cataacccct gaaaacgatc
      181 cggataatat tatccctgcg agaatcaggg catctgtttt aggcgttttg ataccaaact
      241 gtttgatgct gagccaaatt gagattaatg ccagagcgaa aaatgccggt tcgaaataaa
      301 gtgctgtcgt gcgcttgccg ccgaatttaa tgaaattcag cacatagctg ttgctgtaaa
      361 tcagatattt cgaaatcgac tccataatac tgctgccacc ggtgaggata atttgcgcca
      421 tctccaccgc cgccagcgcc accaccagtc cgaccaccag ataaaagaaa cgtaatatct
      481 tacgatggtt gtgtggcgaa attgttttaa aacgaatact ccacaccatg ccaataatga
      541 tcaaaatata gacaaacagc atagttgaag tgacgtattt accggcatcc aacgactgac
      601 caaacaggta gttaaacgcc gtgagtcccg cgccaatccc taaggcaatc atcaattttt
      661 taatgctgat gcgttctaaa aacagcagta acaagacagg taaaaaagtg acgatggtga
      721 tggggaaact ttcgccaagc tgggcgattt tgacgttaac cagcaggtag atcagtggca
      781 gcagcaggta gctacagatt ctgatagaag ttgacatact cctccagcat ctgttgtcca
      841 ctgtaggcgg cgcgactgcg ttggctgaac tcagccagcg tggtaccaaa tatcgcctgc
      901 gcgatttccg gtttgcttaa ctgtaccagt tgcagcacct cttcttcact gacggtttta
      961 ccaccggatt tttgcagcac ttcccgcgcc gcatcgctat gggtggcaat caccggcacg
     1021 ccaatcgata gcgcctcaca caaaatcagc gggtagttat cgacacgaga actgaacacc
     1081 agcgcatcca tctgattgag cgcgctcatc aacttgcgct tgtcagtttc aaagccgtga
     1141 ttaaccacgt tgccagcggt gaacggcgag aacttaccaa aggtatgcag ttcgattttg
     1201 tcgcccagcg ccatcatctc acgcaccagt tgctggttag ttttgccgtc gtaacgcagg
     1261 tcatgcgcca ccactgcgat tttcggcttg ccctgagttt cgcgcaccgg aggcagatct
     1321 gccagaatcg cttcggttgc catatcaatg ccattattga taatccggca acgccctggc
     1381 ccgtacagac tattgaaagc gtcagccaca tgctggctgg gggaaataaa ctgacagccc
     1441 agcgccagca tctcgcggaa taactggcgt ttgcccgcca ccagctggtg cgcgcgatca
     1501 atcttcaccg gcggatagtt atttaaggtc gggcatttct ggcagcccgt tttccagcct
     1561 tcgcaaccgt cggtaaaggc gcagcgtccg gttacactcc agtggtcgtg cagcgtccag
     1621 accagggtga catccggttt atggtttttc actttttcgc aaaagcgcac cacgctgtta
     1681 agattcaacc agtagctgtg cagcacatga aaatgcagga ccaccggacc cggtgtgcga
     1741 gtaatggtgc gatataactc attgaaattg ccgaacagat cgcgattaaa cagacgaaac
     1801 aacgcaatgt tcgccatcgc ggtcatccgc ggcgtatgtt tgatgacctg cggatagttc
     1861 tgatggctga cgctctcttt gccgcctttg ccgtagccgt agacaaaatg tgacgccagc
     1921 ccctgttgca gcgcacgctg gtggagatct aacgccacac ctgctgcccc gccttccgcc
     1981 agtcgcacat taaattgcag aatattcatt taatcacctt cactcgcgct ttttctccca
     2041 ccaccagcgc gttgtccggg acagagtcga gcaccacgct gcccgcaccc acggtgacgt
     2101 tgttaccaag cgtgatatca ccaagaataa tgacgttggc accgagttcg acgccgttgc
     2161 caatgtgtgg acatgccatg ttatcggcac cccgattgcc gatagtgacg ccgtggcgaa
     2221 tggtgaaatc atcccccgcc accacgtttt tattgatcac tacggcgtaa ccgtgatgga
     2281 tagtaaagcg gcggccaatg gtcgcggcgg cctggatttc ataaccgaaa aagcattcgg
     2341 tgataatgcg atacagcacc agcagcgggg ccgcccacag attgttgagg acgttctttt
     2401 tgcgccacac cgaacaaaaa tgagcgacgc gataggcaag taccatgcag cacgggcgta
     2461 aactccagct gttggcgcgc agatcttcca gcatctttag cgcccccgaa taccatcagc
     2521 cagacgtttg ccgttacgca ccgacagcag cgtcaacaag gtgcgccagg tcatacgttt
     2581 attgcggatc tggtagaggg taaacagctg atatttttta ctggcgcggt cgaatttgtc
     2641 tttgtgcttg cggtaaaagt ggaagtatcc ggagaatttt ttcggtgacg aggtgatctg
     2701 catttcgccg tgattgatat gcaggatctg cgtcgcctct tccactttcc acggttcgcc
     2761 gtactccacc accatccgca ggaatatgtc gtaatcctgt gctgctttca gttcggtatc
     2821 gaacaaacac tctttgaaac gccatgccca ggtaaagacc tgattgccaa tgatattacg
     2881 tttatagaac aggcggcgtg agtacggcga tttgggatac agtggtaagc tcgccggttg
     2941 ggaataaact tctccctagc aaacgtagtc gttagcgtac aaaaaggcgt gtgtgaccag
     3001 ttgctgttta tgggcgagga agacgctcag gcggttgggc gtccattcat catcatcatc
     3061 gatcccggtg atgtattccc cttgcgccag cataatcgcc tggttacgca ccgcgcacgc
     3121 cccgctgtta atgtcgttgt ggatgtaagt gatacgcgga tcgttgaggg cagtgacgta
     3181 ctgttgcagt tgttcccagg aggtggagca atcatccacg atgatcatct cccagttgct
     3241 gtagtcctgg cgcaggaccg attttatcgc ccgaatcgcc agttgttggc ggttccaggt
     3301 tggcatatag attgagatca gcggattgtc tttcataggt tgttctcccg gcttatgagc
     3361 tggtttgctt gccggatgcg gcgtgagcgc cttatccggc ctacggggcg gtgcgaatgc
     3421 aggcgtcgtt atttcgcatc cgacttatat tcgtattcgt aatagccata atcctgatac
     3481 gcgctggcgc ggcggaagat ggagttcaga atcacccctt tcaccggaat accgttttgc
     3541 tcaaagcggc tcagactggt ttccacttct ttcaatgtgt tgaccgcata acgcgccacc
     3601 attaacgtgg ttccgacatg acgaccaaca attgccgcat cggtcactgc cagaatcggc
     3661 ggcgtatcaa tcaacaccag gtcatagttt ttactcgccc agttcaccag ttcggcaaag
     3721 cgttcgctca ttaacagttc agaaggattt ggcggcacct gaccgcgcgg gatcaggtca
     3781 aatttggcaa tagaggtcgg tttagcagcg gtagtaatat cgccctgacc aatcagaatc
     3841 tccgacagcc cattgacatt attggtgccc aacagctcgt gggtgtagcc tttgcgcata
     3901 tcgcagtcga tcaacaacac gcgtttattg gtctggctga tcaccgccgc caggttggcg
     3961 cagacaaagg ttttaccgat tgacgggcta accccggtca tcatcaacac attgttctgc
     4021 gcctgcatca tcgcgaagtg cagactggta cgcagactac ggatggcttc aatcgccaga
     4081 tcggttggat tccccactgc cagtagctgg ctctgtttat agcgtttaac ccctttgatg
     4141 gttttgacgc tatcgcgcgc tttctgccac tccgacagcg ggatgctggc atagacgctg
     4201 attccgtgtt cttccagcac ttgcgggctt tcgatgccgc gattaaacaa cgagcgcagc
     4261 agcacaccca cgatagagag catcaggcca aggataatcg cgccgaggat aatcagccct
     4321 ttcttcggtt ttagcacgcc aggctgggtg attgccgggt caataatgcg cacatcgccg
     4381 acggtgctgg cttcggtgat tttcagctcc tgctctttat tcagcagttg catatagacc
     4441 tgctgaccag actcgacatc gcgggtcaga cggacaattt cctgctggtt tttcggcatc
     4501 gccgttacgc gaccgttaag tttggctttc tcttcttcca gcgcctgacg tttctccagc
     4561 agcgtgcggt acgccgggtg aactttggtg tacagcttgg agatttccgc ctctttaaag
     4621 gtcagttcgt tcaactgcgc gtcgatgttc accatcgaat cgagcaccgc tttcgcttca
     4681 agcggcagat caacagaatc tttatcctga cggaaggcat tcagtttgtt ttcggcaaca
     4741 tcgaggcggc tacgtacttc cggtaactgt tgcgcgagga aagcgaggct tttcgacgct
     4801 tccgccgatt tgcgctcaat attttgttcc tgatagttac gagcgatgct gttaagaatg
     4861 tcgcggatct gttcgcgatc ttcaccggta taagtcaggc tcagtacgcc tgcgtctttg
     4921 ccgttctccg ttaccgtcag gctgttttgc agttggttga tcatccccaa cgtggagtat
     4981 ttggtgacgg taaactcact gcccgggcgg gcgtgaatgg cttcaaccat cagcgtgacg
     5041 ccttcttttt tcagcatctg gcccgcttgc ccacgggcgc taaagccgcc atcactgctc
     5101 agggtgtagt ttttgtcatc cagcacatta agcgtaaaca cctgatccgc catctctttc
     5161 gggcggttaa aggtagtcac tttcaccgtc tcgttctgac gccccatcag gcgatcccag
     5221 cccgccccga aaatcgggaa cgtgtttttg ctcaccgcaa tatcgaggtc gagatcgtcc
     5281 accgttttac caagcaccag acgcgaacga atcaactgga tctcggcgtc cgatgcaggc
     5341 ggtttgttgg ctaatgccga gccgatatcc tgcaccagcg aattgccgct gttttgctcg
     5401 atttgtacca gtgcgtcggc actataaatc ggcgtggcga agaaagtgta aaccacggca
     5461 cagagggcaa atacgacggt gatgccaatc acccaccagc gcgcttcaat gacggtgccg
     5521 accaggcgac caatatcgat ttcatcactg cccgttaccg gagcggcatg ttgttttact
     5581 ttttctgtca ttcttatacc tgctctgcgt tcaatgcctg cgcccactgg cgggcagacc
     5641 gttcaagtaa tgtgtacacc gctgcaaacg tttcccggct tttgtgatac ggatcgggga
     5701 tttcacattc gttatcccag tgaccaaaca gcatcacttt gccgcgcatc tccggtgcca
     5761 tctcgcataa gcgttcgata tggcgcttat ccatggtcaa aatcaggtca tagttgcgac
     5821 acaggcggcg gctgatttgt cgtgcacagt ggccttccag agacagttga tgttctgcgg
     5881 cgacgctgat agcggtagga tcagcgccct taccgaccag cgcgccgagt ccagcggact
     5941 ccactttcag ttccgggtga taacgttgca gtaagcgttc cgccatcggg gaacggcaaa
     6001 tattgccgac acagacaact aagatgttgt taaacatgac gattaccagt tatgaatgtc
     6061 gctggctgta tccgtcatgt aacggacacc gctaatagtt ggcagcaact gattgatcag
     6121 acggttccag cgggaaaccg gggcggtggt gacatacacc acgtcataag gttgcaggcg
     6181 gaattctgtc gccatcacca gcgacgtcgc atcggacata tccagctggt agatattggc
     6241 aatcttgccg ttacgcccgc cctcgccttt cagcggacga atgacaaaga tgccgctggc
     6301 gttggaggtg gtcatgtcga taccttcggc attggccaga gcttcagtca gggtcatgcc
     6361 gctaaagtcc attttcaggg tgctctgttt cttcacttca cccatcacaa atactttcag
     6421 atcatcatta cgcggcacgt agagaatatc gccgggataa agcagtcggt tctgattgag
     6481 gtcgccgttt tgcatcagcg cctgtaaaga aatgcgttct tcacgaccat tgtgtgttag
     6541 caccacgttg cgccagtcag cggtgtcggt caggccacct gcggcgttga tggcgtcgag
     6601 aatggtcagt ggtacgttgg tgatcgcctg ttgaccggat ttattcacct gacctgagat
     6661 ataggccttt tgtgagcgga aggcggcgat attaacgtcc acctgcgggt cagcgatgta
     6721 cgtcgctaag cgcccggtaa tatcactgcg gatttcagcg agcgttttcc cgactacgtg
     6781 aaccttgccg atatacgggt aaaacatagt gccgtcaggc tgtacccagt tgccggtgtc
     6841 gctggagctg cggtactgac cagctggcgt ggtgagttcc gggtgatccc agacggtgac
     6901 attaagaacg tcccccggcc cgacgcgata ctggtaattc gcgatctcgc tttccagcgt
     6961 catattgggg cgcgctacat tcgggcgtgg gcgtaattgg tcaatcaggc gcggggtcag
     7021 cggataaaca ttcaccattt tgtcgagatc gaaatcagcg tcctgctgtt tgatgacgtc
     7081 tttgcccatc gtcgacatat tgctgcccgg aagtactgtg caaccgctta tcaaggttac
     7141 tgacaccaat aatggcatca atttcatttt ggatttcatc attgtttatt tatcactttg
     7201 gcagagtaat tatcctgtgc actattaata gcaatgtcgc catgcacatt taccttgcag
     7261 ttaattgaat aaaaatttaa ctggcatcag tcctaaaaaa attgatttca tccgcaggct
     7321 attgacagaa taattcagac tggtctttca ggcatccaga cacgctaccg cccctggctt
     7381 tttagctacc aatacactga ttgagcttaa tttttcaaac cctctctgca tacaatcgtt
     7441 gatgagaaag ggttattgcg gaaattaact tccgaatata aggtgacatt atggtaattg
     7501 aatattggct cgccaataat gcaggaggaa gtgttacagc taacggaata gcaggcaagg
     7561 taacaattcg gcaattggct atttttaaga attatattaa gtatcattca atatggtttt
     7621 taggagtttc tttaggttga caatatttaa tattgcgtct ccacatgcga tatttcttaa
     7681 ataatgtttt attattaccg cttttaattc agggataatg tgaggttatt accctcaaat
     7741 aatatgagat aaatagtgct gcaacattgc atttttgccc cgatttatcc atgatcgaat
     7801 tgtgacattt gtcatacaac gaataggttt tgtacttact atggaatgga ttgccgatcc
     7861 gtctatctgg gccgggttaa tcacgctgat tgtgatcgaa ctggtcctcg gcattgataa
     7921 cctggtcttt attgccatcc tcgccgaaaa actaccgccg aagcagcgcg accgcgcacg
     7981 ggttaccggg ctgctgctgg cgatgttaat gcgcctgtta ctgctagcgt caatctcctg
     8041 gctggtcacc ctgactcaac cgctgttcag cttccgctcg tttaccttta gcgcccgcga
     8101 cttaatcatg ctgtttggtg gtttcttcct gttgttcaaa gccacgatgg agctgaacga
     8161 acggctggaa gggaaagaca gcaataatcc cacacaacgc aaaggtgcga agttctgggg
     8221 cgtggtgacg caaattgtgg tactggacgc cattttctca ctcgactcag tgattaccgc
     8281 cgtcgggatg gtcgaccatt tactggtcat gatggccgcc gtggttatcg ctatcagcct
     8341 gatgttgatg gccagcaagc cgttaacgca atttgttaac agtcacccga ccatcgttat
     8401 tctctgctta agcttcctgt tgatgattgg ctttagcctg gtggcagaag gtttcggctt
     8461 cgtcattccg aaaggctacc tgtacgctgc cattggtttc tcggtgatga tcgaggcact
     8521 caatcagttg gctatcttta accggcgacg ttttctttcc gctaatcaga cgctacgcca
     8581 gcggactacc gaagcggtaa tgcgcctgct tagcgggcaa aaagaagatg cggaactgga
     8641 cgccaaaacc gcgtccatgc tggtggatca tggaaaccag cagatcttta atccgcagga
     8701 acggcggatg attgagcggg tacttaatct taaccagcgt accgtaagca gcattatgac
     8761 gtcgcgccac gatattgagc atatcgatct caacgcgccg gaagaagaaa tccgccagtt
     8821 gctggagcga aatcagcata cgcggctggt ggttaccgac ggtgatgacg cagaagattt
     8881 gctcggtgtt gttcacgtta tcgacctgct acaacagtca ctgcgcggcg aaccgctcaa
     8941 cctgcgggtg ttgattcgcc agccgctggt gttcccggaa accttgccgt tgttaccagc
     9001 cctggagcag ttccgtaatg cccgcacgca ctttgctttt gtggtggatg agtttggctc
     9061 ggtggaaggg attgtgacat taagtgacgt cactgaaacc attgccggta acttaccgaa
     9121 cgaagtggaa gagatcgacg cccgccatga tattcagaag aatgcggacg gttcctggac
     9181 ggcgaatggt catatgccgc tggaggatct ggtgcaatat gtgccgctgc cgctggatga
     9241 aaaacgtgaa tatcacacca ttgccgggct gttgatggag tatttgcagc gtattccaaa
     9301 acctggcgaa gaagttcagg tgggggatta tttgcttaaa acgttgcagg tggaaagcca
     9361 tcgcgtgcag aaggtgcaga ttataccgct gcgtaaggat ggcgagatgg agtacgaggt
     9421 gtgatggtat cggccatttt acccaatgct gcgtaacgcc ttataacttc agcaatgaag
     9481 cgttgtctgt ggtaaacgga catcacagcg ggggtgtggg tccggctgta aatcgccgaa
     9541 tcgtttcctg taggctgggg cagcccacat ggatgtgggc tgagggcgcg ttttacaggg
     9601 atgttacctc gcgcccgtac ccgatagccg aaaggaataa gatgagggaa ccgcgccagc
     9661 ggcgatttac cgccgggagc ccgggttgcc agggtggtgg cgattgagcc accctggcac
     9721 gttcaccggc ttgagcgttc catagtagcg aggaacatca agtgaacgga acagccttcg
     9781 atcttacata ttcagaatat aatagtgccg ccgcgaacgc ctgatgcgac gctttacgcg
     9841 tcttatcagg cctacaggac aggtcacctg gtaacctaca tcttctccag caacttcttc
     9901 acatctttgc cattgcggga atctttattc cgctcggccc agtcattcag gcgacgtttc
     9961 gcttcgtcct gtagatgttt gcgcagtaac tgatccactt gcaggctgta attgagttgc
    10021 tgccagttgc cataaacccg cagcggtact ggcgtttctt tgaggaaatc aatcagtttg
    10081 ctttccccgt tccagccacc cacgacccga atatcaaact gggtgtcgca ggtttgatct
    10141 gccagattca acatgccttc ccctgtcagc gccagcacag gcgattgacc ttgcatgtcg
    10201 tttaacgtca cgacgccatc cttcaacgtc aaatcggtgg taaagcggtc aagccgcgtc
    10261 acgttatcga agttttcagc ggccttcaca tcaccgccat tacgctctac cgcttgctga
    10321 atcatctgct ggaagttcat cccttccata cgtgtgtcgg tcatctcaac atgcgcctgt
    10381 ccttgccagt tgtggcggaa tgcgtcggca tctatgtcag caccggagaa atcaccagcc
    10441 agtgacattt ttccggtcaa cgaaatcgga tagttaaacg ccttcagaat ggtaccaatc
    10501 tcaacgttct ccagccgtgg ctggaagttt atccgcggat ttattgatgt cgcgtccagc
    10561 gtgcccggca gtgaaacctt tccaccgtta agcttgccct gcagttgagt aatttccagc
    10621 aaacccgact tgttggtcat ttgcgtggca acatctgtaa aattcattcc gcgccagcgc
    10681 acgttactgg cctgcaacaa aatatcagct gtaaagcctt gcagtccctg ataggccggt
    10741 tcatcaatac gcgaagaaat gaccgggcgc ggcaacgttg attggctctg cccctgctgc
    10801 gcggcaccgt tttcaccatt cgcggtttca ttaagcggga tgaggttgtc aagattcagt
    10861 tgcgggaatt gcagtctcag ctgccattcc ggtttctctg tcagcgtgac ctgtgcttgt
    10921 ccgctcagcg tactgtcatt ggcggtcaaa ctaatttggt taaatgaaag gcgtttatgt
    10981 gactcctgcc actgggactg gaaactcccc tgcccctgaa taccttgttt tggtaaatcg
    11041 gcaccctgca actgccagtt aatttgttca atagccgctg ttaaatcatg cggataatca
    11101 gaagcatcta ccgtaccgtt aagggatatc gtgagatcgc gctgatcgcg attaacccgc
    11161 ccggagaact caaatgagcc acgatgttgg ggatcttgtt ccatttgcag gcggatattg
    11221 cggattgtca cctgctcgtc atcttcatgc tggaacacca gaacgctgtc cgccacctta
    11281 agactggata tatcaaacga ccatccgcga tcgtctgaca gatccggcaa ggtattgtcg
    11341 cgcggtgcaa ccggagcgtc ttcactgcgc accgcttccg tctgcggcgt cagttggatc
    11401 actgcccctt ttagcatcac ctgcttaacg ctcagttgat gactcagtag tggtaaaagc
    11461 gccacgtcca gacgcatgtt gtcggcgcga accagtggct ggcttgcgcc ctgggcggtg
    11521 agagacattc gcccggagag gatactaagc tgcggccaga cgtgccaacg cagtggcccg
    11581 tcgagctgca attgataacc gctacgtgca gcaacttgct tgaccatata gtcgcggaaa
    11641 tcattcggat tcaccagcaa cactaacgca gataacccgg ccaccagcac gaccaagagt
    11701 atcatcagcg tcgtcagaaa tcgtctcatg gtatcctcaa tgggcctgaa ttagtcttta
    11761 tcgattcggc tggctaccgc gccctgctgg ttgcgatatt tcgcatcttc acggcggttg
    11821 taaggtcgcg ccgccgggcc ggaaagcggc tcaaagctca gcgcaccaat taacatgccc
    11881 ggacgcagcg ccagcggcag cttaccggag ttgtagaact ccagcacaat gcaaccagac
    11941 cagcccggat cgatacggtg cgcggtgacg tgcaccatca gccccagacg cgccaaagag
    12001 gaacgcccgt ccagccagcc caccagatcg gctggcagcg tcaccgactc cagtgtcacc
    12061 gccagcgcca gctctcctgg gtgaagatag aacgcctcgc cctcgtcgag aacgatctca
    12121 tcgctcatca cgcggtcaag cgcggcgctc acttcatctt tgggaccgct cagatcgata
    12181 aacgctgccg tgtgaccacg gaaggtacga aatttattgc ccaggcgtac atccaccgtc
    12241 gcgccgttaa tacgctccac tggcggacgt gggttgatcg acaaacggcc ttcatcaagc
    12301 caggcttcaa tatctcggtc acacagacgc atggcatttt ctcctttcgc acatcactcc
    12361 cttaacgcca attacgtcaa gggcatacca acttatcact gaacggtaca caatttatca
    12421 agcttattca aagaactgac ttattttcgc tttcaatata tcgatcgcga tgcggttttt
    12481 cccgccgcgc ggcacgataa tgtccgcata ttgtttagaa ggctcaatga attgcaggaa
    12541 catcgggcgc acggtttttt gatattgcgc catcacggaa tccattgaac gcccacgctc
    12601 gttaacgtca cgcttgatgc ggcgcatcag gcagatatcc agcggggtat caacgaaaat
    12661 ggagaagtta agttcgtcac gcaaacgcgc atccgtcagc aacaaaatgc cttcgagaat
    12721 gatgaccttc ttcggctcaa ccgtcaccgt ttctttcata cgcgtatgtt caacatagct
    12781 gtaaaccggc aggtcaattg ccgagccgcg tttcaacgct tgtaaatgct caagcaacag
    12841 gctgtgatcc atcgcgctgg gatggtcgta gttggtctta acgcgttctt ccatcgacag
    12901 atggctttga tctttgtaat agcagtcttc gggaattacg ccgatgtgtt catcaccgac
    12961 ttgctcacgt aattcacgat aaagggtact ggcaataaga ctcttgccgg aagccgatgc
    13021 gccagcgata ccgataatga cgcactgatg agactgatca gtcatatatt tagcgacctg
    13081 attaacctgg atgttaggaa gggggcgacg aagcgccaaa cgcggcaatt atagggattt
    13141 catccgtctg ataccagtcg aatagcgttg ccgcgcgctc agagttaatt gttgacaaag
    13201 aattcccggt ggcaaattac gttgatcagt tttatgtaag gtaaaaaatg ttatacgcag
    13261 ttgcgcaaat tatccgcctt tacgtcactt tatgagcaat tcgcatataa aatgtaaaac
    13321 ttttgtacta gcataaacac agaaacgaat actggcgacc tggccttgcg gataaagcgg
    13381 taataagcaa acaatcacag catgtattaa ttgccctgcc ccacccgctg cttcacctgg
    13441 tcagtttagg tttagtctcg tttatcttta cccttgtctc gcttgagctt tcgcagtttg
    13501 gcacccaact cgccccactg tggttcccga cgtccatcat gatggtggcg ttttatcgcc
    13561 atgccgggcg catgtggccg ggaattgcgc tgagctgttc gctgggaaat atcgccgcat
    13621 ccattccagg aaggccagca ggtcaaagca ggcgatttac tggcagaaat tgaccccagc
    13681 cagttcaaag ttgcattagc acaaacccag ggccaactgg caaaagataa agccacgctt
    13741 gctaacgccc gccgtgatct ggcgcgttat cagcaactgg caaaaaccaa tctcgtctcc
    13801 cgtcaggagc tggatgccca acaggcgctg gtcagtgaaa ccgaaggcac cattaaggct
    13861 gatgaagcaa gcgtcgccag cgcgcagttg caactcgact ggagccgcat caccgcacca
    13921 gtcgatggtc gcgttggtct caagcaagtt gatgttggta accaaatctc cagtggtgat
    13981 accaccggaa ttgtggtgat cacccagacg catcctatcg atttagtctt taccctgccg
    14041 gaaagcgata tcgctactgt agtgcaggcg caaaaagccg gaaaaccgct gatggtagaa
    14101 gcctgggatc gcaccaattc gaagaaatta agtgaaggca cgctgttaag cctcgataac
    14161 caaatcgatg ccacaaccgg tacgattaaa gtgaaagcac gctttaataa tcaggatgat
    14221 gcgctgtttc ccaatcagtt tgttaacgcg cgcatgttag tcgacaccga acaaaacgcc
    14281 gtagtgatcc caacagccgc cctgcaaatg ggcaatgaag gccattttgt ctgggtgctg
    14341 aatagcgaaa acaaggtcag caaacatctg gtgacgccgg gcattcagga cagtcagaaa
    14401 gtggtgatcc gtgcaggtat ttctgcgggc gatcgcgtgg tgacagacgg cattgatcgc
    14461 ctgaccgaag gggcgaaagt ggaagtggtg gaagcccaga gcgccaccac tccggaagag
    14521 aaagccacca gccgcgaata cgcgaaaaaa ggagcacgct cctgatgcag gtgttacccc
    14581 cgagcagcac aggcggcccg tcgcgcctgt ttattatgcg tcctgtcgcc accacgctgc
    14641 tgatggtggc gatcttactc gccgggatta tcggttatcg cgccctgccc gtttcggcgc
    14701 tgccggaagt ggactatccg accattcagg tggtcacgct ctacccaggt gccagcccgg
    14761 atgtcatgac ctctgccgtt accgcgccgc tcgaacgcca gttcgggcag atgtctggcc
    14821 tgaaacagat gtcgtcgcaa agttccggcg gtgcgtcagt tatcactttg cagttccagc
    14881 taacattacc gctcaatgtc gccgagcagg aagtgcaggc cgcgattaac gctgcgacca
    14941 acttgttgcc gagcgatctg cctaacccgc cggtttacag caaagtgaac ccggcagatc
    15001 cgccgatcat gacgctcgcc gtcacctcaa ccgccatgcc gatgacgcaa gtggaagata
    15061 tggtggaaac ccgcgtcgcg cagaaaatct cgcagatttc cggcgtcggc ctggtgacgc
    15121 tttccggcgg tcagcgtccg gctgttcgcg tcaaacttaa cgctcaggcg attgccgccc
    15181 tcggcctgac cagcgaaacc gtgcgcaccg ccattaccgg cgctaacgtt aactcggcaa
    15241 aaggtagcct cgacggccct tcccgtgcgg tcacgctttc cgcgaacgac cagatgcaat
    15301 ccgccgaaga gtatcgccag ctaatcatcg cctaccagaa cggcgcgcca attcgtctgg
    15361 gcgatgtcgc aactgtagag caaggtgcag aaaacagctg gctcggcgcg tgggcgaaca
    15421 aagaacaggc cattgtgatg aatgttcagc gccagcccgg tgctaacatt atctccaccg
    15481 ccgacagcat tcggcagatg ctgccacagc tcactgagag tctgccgaaa tcggtgaagg
    15541 tgacagtgct ttccgatcgc accaccaata tccgcgcatc cgtcgatgat actcagtttg
    15601 aattgatgat ggctatcgcg ctggtagtca tgattatcta cctgtttttg cgcaatattc
    15661 cggcgaccat cattcccggt gttgctgtac cgctgtcgtt aatcggcact ttcgcggtta
    15721 tggtgtttct cgatttttca atcaataacc tgacactgat ggctttaact atcgccaccg
    15781 gattcgtggt cgatgacgcc atcgtggtga tcgaaaacat ttcccgctat atcgaaaaag
    15841 gcgaaaaacc gttggcggcg gcgctcaagg gcgcaggtga aatcggcttt accattatct
    15901 cgctgacctt ctcactgatt gcggtgttga tcccactgct gtttatgggc gatatcgtcg
    15961 ggcgactgtt ccgcgaattt gctattaccc tggcggtagc gattttgatc tcagcggtgg
    16021 tgtcgctgac cctgacaccg atgatgtgcg cgcggatgct cagccaggag tcgttgcgta
    16081 aacagaacag cttctcccgt gcctcggaaa aaatgttcga caggataatc gccgcctatg
    16141 gtcgtggact ggcgaaagtg ctgaatcatc cgtggctgac cttaagcgtg gcactcagca
    16201 cgctgctgct tagcgtgctg ctgtgggtgt tcattccgaa aggtttcttc ccggtacagg
    16261 acaacggcat tattcagggc actttgcagg caccgcaatc cagctccttt gccaatatgg
    16321 cccagcgaca acgccaggtc gcggacgtga ttttgcagga tccggcagtg caaagcctga
    16381 cctcatttgt tggcgttgat ggcactaacc cgtcgctgaa tagtgcacgt ttacaaatca
    16441 acctcaaacc gttggatgaa cgtgatgatc gggtgcaaaa agtcatcgcc cgtctgcaaa
    16501 cggcggtaga taaagtgccg ggcgtcgatc tcttcctgca accaacgcag gacctgacca
    16561 tcgatactca ggtcagccgc acccagtacc agtttacctt gcaggccacg tcactggatg
    16621 cgctcagtac ctgggtgcca cagttgatgg aaaaactcca gcaactgcca cagctttctg
    16681 atgtctccag cgactggcag gacaaagggc tggtggcgta tgtcaatgtt gatcgcgaca
    16741 gcgccagccg tctggggatc agcatggcgg acgtcgataa cgccctgtac aacgcgtttg
    16801 gtcagcgact gatttccact atttatactc aggctaacca gtatcgcgtg gtgctggagc
    16861 acaataccga aaatacccca ggcctcgcgg cgctggatac cattcgcctg accagcagcg
    16921 acggtggcgt ggtgccgtta agctcaattg ccaaaattga gcagcgtttt gcgccgcttt
    16981 ccatcaacca tctggatcag ttcccggtaa caaccatctc ctttaacgtg ccggataact
    17041 attcgctggg cgatgcggtg caggcgatta tggacaccga aaagacgctg aatctgccgg
    17101 tggatatcac cacgcaattc tagggcagca ccctcgcctt ccagtcggcg ctgggcagca
    17161 ctgtctggct gattgtcgcg gcggtggtgg cgatgtatat cgtgctcggc attctgtacg
    17221 agagctttat tcacccgatc accattctct cgacgctacc caccgcaggg gtcggcgcgc
    17281 tgctggcatt aatgattgcc ggtagcgaac tggatgtgat tgcgattatc ggcattattt
    17341 tgctgatcgg tattgtgaag aagaacgcca tcatgatgat cgacttcgcg ctggctgctg
    17401 agcgcgagca aggcatgtcg ccgcgcgatg caatctacca ggcttgtctg ttgcgttttc
    17461 gtccgatcct gatgaccacc ctggcggctc tgcttggcgc gctgccgctg atgttgagta
    17521 ccggggtcgg cgcggaactg cgtcgtccgt taggtatcgg catggtcggc ggtctgattg
    17581 tcagccaggt gctgacgctg ttcaccacgc cggtgattta tctgctgttc gaccgcctgg
    17641 cattgtggac caaaagtcgc tttgctcgcc atgaagagga ggcgtaagtg aagttttttg
    17701 ccctattcat ttaccgcccg gtggcgacga ttttactgtc ggtcgccatt accctgtgcg
    17761 gcatactggg tttccgtatg ctgccggtcg ccccgctgcc gcaggtcgat tttccggtga
    17821 ttatggtcag cgcctcgctg cccggtgcgt cgccggagac aatggcgtct tccgttgcca
    17881 cgccgctgga gcgctcactt gggcgcattg ccggggttag tgaaatgacc tccagcagtt
    17941 cgctcggcag cacgcgcatt attttgcagt ttgattttga ccgggatatc aacggcgcag
    18001 cgcgtgatgt gcaggcggcg atcaacgctg cacaaagttt gctgcccagc gggatgccca
    18061 gccgcccgac ctatcgcaaa gcgaacccgt cggatgcgcc aattatgatc ctcacgctga
    18121 catccgatac ttattcgcag ggtgaactgt acgatttcgc ctcgacgcag ctggctccga
    18181 cgatttcgca aatcgacggt gtcggtgatg tcgatgtcgg tggcagctcg ctgcccgccg
    18241 tgcgcgtcgg gctgaccccg caggcgctgt ttaatcaggg cgtgtcgctg gacgacgtac
    18301 gcactgccat cagcaatgcc aacgtgcgta aaccgcaggg ggcgctggaa gatggcactc
    18361 accgctggca gatccagacc aacgatgaac tgaaaaccgc cgctgaatat cagccgctga
    18421 ttattcacta caacaacggc ggcgcggttc gtctgggcga tgtggcgacg gtgactgact
    18481 cagtgcagga tgtgcgcaac gccgggatga ccaacgccaa accggctatt ttactgatga
    18541 tccgcaaact gccggaagcc aatattatcc agacggtaga cagcatccgg gcaaaattac
    18601 cagagttgca ggagaccatt ccggcggcga ttgatctgca aattgcccag gatcgctccc
    18661 ccaccattcg cgcctcgctg gaagaagtcg agcaaacgct gattatctcg gtggcgctgg
    18721 tgattctggt ggtcttttta ttcctgcgct cgggtcgcgc cactattatt cccgccgttg
    18781 cggtgccggt ttcgctgatt ggtacgtttg cggcgatgta cctgtgcgga ttcagtctca
    18841 ataatctttc gctgatggcg ctcaccattg ctactggttt cgtggtggat gacgccattg
    18901 tggtgctgga aaacattgcc cgtcatctgg aagcggggat gaaaccacta caggccgcgc
    18961 tgcaaggtac tcgcgaagtc ggctttacgg tgctgtcgat gagtctgtca ctggtggcgg
    19021 tgttcctgcc gctgctgttg atgggcggat tgccgggccg actgttacgc gagtttgccg
    19081 tgacgctttc tgtcgccatt ggtatttcgt tgctggtttc cctgacgcta acgccaatga
    19141 tgtgcggctg gatgctaaaa gccagcaagc cgcgtgagca aaagcgactg cgtggttttg
    19201 gccgcatgtt ggtagccctg caacaaggct acggcaagtc gctgaaatgg gtgctcaatc
    19261 atacccgtct ggtgggcgtg gtgctgcttg gcaccattgc gctgaatatc tcgatcccga
    19321 aaaccttctt cccggagcag gacaccggcg tactgatggg tgggatacag gcggatcaga
    19381 gtatttcgtt tcaggcgatg cgcggcaaat tgcaggattt catgaaaatt atccgtgacg
    19441 atccggcagt ggataatgtc accggcttta ccggcggttc acgagttaac agtgggatga
    19501 tgtttatcac cctcaagcca cgcgacgaac gcagcgaaac ggcgcagcaa attatcgacc
    19561 gtctgcgcgt gaagctggcg aaagaaccgg gggcgaatct gttcctgatg gcggtacaag
    19621 atattcgcgt tggtgggcgt cagtcgaacg ccagctacca gtacacgttg ttatccgacg
    19681 acctggcggc actgcgcgaa tgggagccga aaatccgcaa aaaactggcg acgttgccgg
    19741 aactggcgga cgtgaactcc gatcagcagg ataacggcgc ggagatgaat ctggtttacg
    19801 accgcgacac catggcacgg ctgggaatcg acgtacaggc cgccaacagt ctgttaaata
    19861 acgccttcgg tcagcggcaa atctcgacca tttaccagcc gatgaaccag tataaagtgg
    19921 tgatggaagt ggatccgcgc tatacccagg acatcagtgc gctggaaaaa atgttcgtta
    19981 tcaataacga aggcaaagcg atcccgctgt cgtatttcgc taaatggcaa ccggcgaatg
    20041 ccccactatc ggtgaatcat cagggattat cggcggcctc gaccatctcg ttcaacctgc
    20101 cgaccggaaa atcgctctcg gacgccagtg cggcgatcga tcgcgcaatg acccagcttg
    20161 gtgtgccttc gacggtgcgc ggcagttttg ccggcacggc gcaggttttc caggagacga
    20221 tgaactcgca ggtgatcctg attatcgccg ccatcgccac ggtgtatatc gtgctgggta
    20281 tcctttacga gagttacgta catccgctga cgattctctc caccctgccc tcggcgggcg
    20341 ttggagcgct gttggcgctg gagctgttca atgccccgtt cagcctaatc gccctgatag
    20401 ggatcatgct attaatcggc atcgtgaaga aaaacgccat tatgatggtc gattttgcgc
    20461 ttgaagccca acggcacggt aacctgacgc cgcaggaagc tattttccag gcctgtctgc
    20521 tgcgttttcg cccgattatg atgaccaccc tggcggcgct gtttggcgcg ctgccgctgg
    20581 tattgtcggg cggtgacggc tcggagctgc ggcaacccct ggagatcacc attgtcggcg
    20641 gtctggtgat gagccagctc ctgacgctgt ataccacgcc ggtggtgtat ctctttttcg
    20701 accgtctgcg gctgcgtttt tcgcgtaaac ctaaacaaac ggtaaccgag taaatgacag
    20761 atcttcccga cagcacccgc tggcaattat ggattgtggc tttcggcttc tttatgcagt
    20821 cgctggacac caccatcgta aacaccgccc ttccctcaat ggcgcaaagc ctcggggaaa
    20881 gtccgttgca tatgcacatg gtcattgtct cttatgtgct gaccgtggcg gtgatgctgc
    20941 ccgccagcgg ctggctggcg gacaaagtcg gcgtgcgcaa tattttcttt accgccatcg
    21001 tgctgtttac tctcggttca ctgttttgcg cgctttccgg cacgctgaac gaactgttgc
    21061 tggcacgcgc gttacagggc gttggcggtg cgatgatggt gccggtcggc aggttgacgg
    21121 tgatgaaaat cgtaccgcgc gagcaatata tggcggcgat gacctttgtc acgttacccg
    21181 gtcaggtcgg tccgctgctc ggtccggcgc tcggcggtct gctggtagaa tacgcgtcgt
    21241 ggcactggat ctttttgatc aacattccgg tggggattat cggtgcgatc gccacattga
    21301 tgttaatgcc gaactacacc atgcagacgc ggcgctttga tctttccggc tttttattgc
    21361 tggcggttgg catggcggta ttaaccctgg cgctggacgg cagtaaaggt acaggtttat
    21421 cgccgctggc gattgctggc ctggtcgcag ttggcgtggt ggcactggtg ctttatctgc
    21481 tgcacgccag aaataacaac cgcgccctgt tcagtctgaa actgttccgc actcgtacct
    21541 tttcgctggg cctggcgggg agctttgccg gacgtattgg cagtggcatg ttgcccttta
    21601 tgacaccggt tttcctgcaa attggcctcg gtttctcgcc gtttcatgcc ggactgatga
    21661 tgatcccgat ggtgcttggc agcatgggaa tgaagcgaat tgtggtacag gtggtgaatc
    21721 gctttggtta tcgtcgggta ctggtagcga ccacgctggg tctgtcgctg gtcaccctgt
    21781 tgtttatgac taccgctctg ttgggctggt actacgtttt gccgttcgtc ctgtttttac
    21841 aagggatggt caactcgacc cgtttctcct ccatgaacac cctgacgctg aaagatctcc
    21901 cggacaatct ggcgagcagc ggaaacagcc tgctgtcgat gattatgcaa ttgtcgatga
    21961 gtatcggcgt caccatcgcc gggctgttgc tggggctttt tggttcgcag catatcagcg
    22021 tcgacagcgg caccacacaa accgtcttta tgtacacctg gcttagcatg gcgtttatca
    22081 tcgcccttcc ggcgttcatc tttgccagag tgccgaacga tacgcatcaa aatgtagcta
    22141 tttcgcggcg aaaaaggagc gcgcaatgaa gttctggcga cccggtatta ccggcaaact
    22201 gtttctggcg attttcgcca cctgcattgt cttgctgatc agtatgcact gggcggtgcg
    22261 tatcagtttt gagcgtggct ttattgatta catcaaacac ggcaatgaac agcgattaca
    22321 gctgttaagt gatgcgcttg gcgagcagta tgcgcagcat ggtaactggc gcttcctgcg
    22381 caacaacgat cgctttgtct ttcagatcct gcgttcattt gaacacgata attcggaaga
    22441 taaacccggc ccgggtatgc cgccgcacgg ctggcgtacc cagttctggg tggtcgatca
    22501 aaacaacaaa gtactggttg gtccgcgagc gccgattcca cctgacggta cacggcgacc
    22561 cattctggtc aacggtgcgg aagtgggcgc ggtgattgcc tcccctgttg agcggctgac
    22621 ccgcaatact gatatcaatt tcgataaaca acagcggcaa accagctggc tgatcgtcgc
    22681 cctggcaacg ttactcgcgg cgctcgccac ctttctgctg gcgcgcggtt tgctggcacc
    22741 ggtaaaacga cttgtcgatg gcacgcacaa actggcggcg ggcgatttca ctacccgcgt
    22801 aacgcccacc agtgaagatg aactgggcaa actggcgcaa gacttcaacc agctcgccag
    22861 cacgctggag aaaaaccagc aaatgcggcg cgattttatg gccgatattt ctcacgaact
    22921 gcgtacgcca ttagcggtgc tgcgcggtga actggaagcc attcaggatg gcgtgcgcaa
    22981 attcacgccg gagacggtgg cgtctttaca ggcggaggtc ggtacactga ccaaactggt
    23041 tgacgatctt catcagttgt cgatgtctga tgaaggcgct ctcgcctatc aaaaagcacc
    23101 ggtagatttg atcccactgc tggaagtggc gggcggcgca tttcgcgaac gattcgccag
    23161 ccgtggcctg aaactgcaat tttccctgcc agacagtatc accgtatttg gcgatcgcga
    23221 ccgtttaatg cagttattca ataacttact ggaaaacagc ctgcgctaca ctgacagcgg
    23281 cggcagcctg aaaatctctg ccgagcagca cgacaaaacg gtgcgcctga cctttgccga
    23341 cagtgcgcca ggtgtcagtg acgatcagct acaaaaattg tttgaacgtt tttatcgcac
    23401 cgaaggttcc cgcaaccgcg ccagcggcgg ttccgggctg gggctggcga tttgcctgaa
    23461 cattgttgaa gcccataatg gccgcattat tgccgcccat tcgccttttg gcggggtaag
    23521 cattacagta gagttaccgc tggaacggga tttacagaga gaagtatgac cgagttacca
    23581 atcgacgaaa acacaccgcg tattttgatc gtggaagatg aaccgaagct ggggcagttg
    23641 ctcattgatt atctgcgtgc tgcgagctat gcgccgacgc ttatcagcca cggcgatcag
    23701 gtactggcgt atgtgcgcca gacaccaccg gatctgatcc tgttggatct gatgctccct
    23761 ggcaccgatg gcctgacatt atgccgggaa attcgtcgtt tttccgatgt tccgatcgtg
    23821 atggtgacgg caaaaatcga agagatcgac cgcctgctgg ggctggagat tggcgccgat
    23881 gactacatct gcaagccgta cagcccacgg gaagtggtag cgcgtgtcaa aaccattttg
    23941 cgccgttgta aaccgcaacg cgaacttcag caacaggatg ccgaaagccc gttgattatc
    24001 gacgaaggtc gttttcaggc ttcatggcgc ggtaaaatgc ttgacctgac gcctgcggaa
    24061 tttcgtctgc ttaaaacgct ctctcacgaa ccaggaaaag tgttctcccg cgagcaattg
    24121 ctcaatcatc tttatgacga ctaccgcgta gtaaccgacc gcaccattga cagccacatt
    24181 aaaaatctgc gccgcaagct ggaatctctc gacgccgaac agtcatttat ccgcgccgtt
    24241 tacggcgttg gttaccgctg ggaagccgac gcctgccgca tcgtttagtt ttagcgacat
    24301 tactttgtta gccggagatg gcgttcagac ccgatccggc aacattattc cacgcatggt
    24361 cagcaaactg acattatctc tccgccctgg caggcatcag aaaatcgctc atactttaat
    24421 cagtaaacag cacctttaga tgctgttttc gataagcaat ttcaatcaag gagtcattat
    24481 ggctggttgg tttgaactca gtaagagcag tgataatcag ttccggtttg tgctaaaagc
    24541 gggcaatggt gagactatcc tcaccagcga gctgtatacc tcaaaagcct ctgcggaaaa
    24601 gggcatcgcg tcggtgcgta gcaacagccc gcaagaagag cgctatgaga aaaaaacggc
    24661 aagcaacggc aaattctatt tcaatctgaa agccgctaat catcaaatta tcggctccag
    24721 ccagatgtac gccaccgcgc aatctcgtga aaccggaatt gcctccgtta aagccaatgg
    24781 cacaagccag acggtgaaag acaatacgta attacgatgc cgggcgtggg ttgttggcgt
    24841 ccggcgcttt accagatccc ccctacagcg ctacaatgcc cgcccttaaa gtgggggcct
    24901 cccctaaccg cttcatcagg tgaagcggat ctgacctgtc atcagaacga gagaattatg
    24961 tttaaaccgg aactcctttc cccggcggga acgctgaaaa atatgcgtta cgctttcgct
    25021 tatggcgcag atgctgttta tgcgggccag ccgcgttact ccctacgtgt gcgcaacaac
    25081 gaattcaacc acgaaaatct tcagctcggc atcaatgaag cccacgcgct ggggaaaaag
    25141 ttttatgtcg tggtcaacat tgcaccgcac aacgccaagc tgaaaacctt tatccgtgac
    25201 ctgaaaccgg tggtggaaat ggggccggat gcgctgatta tgtccgatcc agggctgatt
    25261 atgctggtgc gtgagcactt ccctgaaatg ccgatccacc tctcggtgca ggctaacgcc
    25321 gtgaactggg cgacggtgaa attctggcag caaatgggac tgacccgcgt gatcctctct
    25381 cgcgagctgt cgctggaaga gattgaagag atccataatc aggtgccgga tatggagatc
    25441 gagatcttcg ttcacggcgc actgtgcatg gcctactccg gtcgctgcct gctctctggc
    25501 tatatcaaca agcgcgaccc gaaccagggc acctgcacca acgcctgccg ctgggagtac
    25561 aacgtccagg aagggaaaga agatgacgtt ggcaacatcg tacacaagta cgagccgatt
    25621 ccggtgcaaa atgttgagcc gacgctgggt atcggcgcgc caaccgacaa agtgtttatg
    25681 atcgaagaag cccagcgtcc gggcgagtat atgaccgcgt ttgaagatga gcacggcact
    25741 tacatcatga actcgaaaga tctgcgcgcc atcgcccatg tagaacgcct gaccaaaatg
    25801 ggcgtgcatt cgctgaaaat cgaaggtcgt accaaatctt tctactattg cgcacgcacc
    25861 gcacaggttt accgcaaagc tatcgatgac gccgctgcgg gaaaaccgtt cgataccagc
    25921 ctgctggaaa ctctggaagg tctggcgcat cgtggctata ccgaaggttt cctgcgtcgt
    25981 catactcacg acgattatca gaactacgaa tacggttatt cagtttctga ccgccagcag
    26041 tttgttggtg agtttaccgg tgagcgcaag ggggacctcg cagcggtagc ggtgaaaaat
    26101 aaattctccg ttggcgacag tcttgagctg atgacgccgc aaggcaacat taactttacc
    26161 cttgagcaca tggaaaacgc caaaggcgaa gctatgccgg tcgcaccagg cgatggttat
    26221 actgtgtggc tcccggtccc gcaggatctt gagctaaatt acgcgctgct aatgcgtaat
    26281 ttctccgggg aaaccacgcg taacccccac ggtaagtgat taatttcgat tatttttccc
    26341 ggatggaaaa ttcttagaaa ccgatcacat acagctgcat ttattaaggt tatcatccgt
    26401 ttcgctgaaa aacataatcc ataaaatgct agctgtacca ggaaccacct ccttagcctg
    26461 tgtaatctcc cttacacggg cttatttttt gtacataagc tgtaaagaag gattgatccc
    26521 tgataaccgt cttccattga ccacatcgat agaatccccc ttcatagcac aatgcctttc
    26581 acgtaacggt atcgtgctcg cccaggttcc ggctacgcac agccagaacg cgcatgtttg
    26641 acgcttacaa aaaaatattc tcactctcca catttgaatg tcagacgagc gacgccatgt
    26701 aatcctgcac cttctgtctt caggtcaact atctgcattt ttttgccctg agtaacacag
    26761 aaatgggctg catcattttt tactatattt tctgcaccag atattctgcc cctggctaaa
    26821 gaagcttcgg cttcggtgta gtattggtta tcgagtttac gctgaatatt acttttatat
    26881 gcaagaccaa atttaccgat acttgtttca tcattatgca cagcacaacc agatataaga
    26941 aaaatactaa ttaatgatat agcagctatc tttttcatct caccttcccc cattaaatac
    27001 caacgacact ctctagtgtt taaatataat aatggcatga ttattataat tgaataggat
    27061 tataataaat gttctgtaca acatttccta cataagtagg aattacggac attgaggccc
    27121 ttcagggtaa ctccatgggg gctttaatat attatattga agatgccact gtttagttga
    27181 atattaggta tatgctcttt tttgaaattt atcggtggca gccgttagta ttcgctgtcc
    27241 ccattgcaag ctcctggtgg taaccactga atcctccata cttgaactga ctttttatcc
    27301 tccgactttc atcctgttct gactccacct tttgttttct gctctacact atctacagac
    27361 caatcataaa ggcacatacg atcatggcag aatttcccgc cagcttactg attcttaatg
    27421 gtaaaagtac tgacaatcta cccttgcgcg aagcaattat gctgttgcgt gaggaaggaa
    27481 tgacgatcca tgtgcgggtc acctgggaga aaggtgatgc cgcacgattt gtagaggagg
    27541 cccggaagtt gggcgtcgca acggtgattg ccggtggtgg cgatggcacc attaatgaag
    27601 tttctacggc gttgattcag tgtgaggggg atgacatacc cgcgctggga attttgccat
    27661 taggaaccgc caatgatttt gccaccagtg tagggattcc tgaggcactg gataaggcgc
    27721 tgaaactggc aattgccggt aatgccattg cgatagatat ggcgcaggtc aacaaacaaa
    27781 cctgttttat taatatggcg acaggcggat ttgggacgcg tattaccaca gaaacgccgg
    27841 aaaaattaaa agccgcgctg ggtggcgtct cttacatcat tcatggctta atgcgcatgg
    27901 atactctgca accggaccgt tgtgaaatcc gcggtgaaaa ctttcactgg caaggtgacg
    27961 ccctggtcat tggtattggt aacgggcgtc aggccggtgg cggtcaacaa ttgtgcccga
    28021 acgcgttaat taacgatggc ttgctgcaac tgcgcatttt taccggcgat gaaattcttc
    28081 cggctctcgt atcaacctta aaatctgacg aagataaccc gaatattatc gaaggcgctt
    28141 cgtcgtggtt tgatatacaa gccccacacg aaatcacttt taatcttgat ggcgaaccgt
    28201 tgagtgggca aaacttccat attgaaatac ttccggcggc gttgcgttgt cgattaccac
    28261 cagattgtcc attattgcgt taatcaaact attctctctt gaaccaggga gaatccgtaa
    28321 tgccggatgc ggtgtaaaca ccttatccgg catacagaac aatactacac cgtaattaat
    28381 tttacccgtg tcttctgcaa tgccaattta tccacctctg acacagcatc atcggtaaca
    28441 ataatgtcaa ttttttcgat tggcagtacc tgattaaaac cgcgacgatt aaatttcgat
    28501 gaatcgagaa cggcaatcac tttatttgcg gcggtgacca tcgccccgct aatcgaataa
    28561 ccttcattaa aggtcgtaat accattaacc gcatcaatac catcagcacc gacaaacatt
    28621 aaatcggcat taatatcctg caatgaacgc tcggcaatgg aaccatgcat cgagcgcgtt
    28681 ttatggcgga ccgtgccacc acagacgacc agagtaatgt ctttattttc ggaaagggca
    28741 aacgctgccg ggagactgtt ggtaatcacc gtgatatttt tagcggtcat taatccttcg
    28801 gcgataagca ttgtggtact tccgctgtcg agaataacgg tcatcccttc atggatcatt
    28861 ttgactgccg cctgcgctat tctgtttttt ggcgcgctgg cgagctgaaa tcgctcttta
    28921 aacccgactt cctgggtctc ggtttcacta ttaccggaca ttattttcgc cgcaccgcca
    28981 tgaaagcgcg taaccacgcc tttttgttcg agaaagcgca aatcggcacg gattgtcgct
    29041 tccgaggcag caaatactcc cgccagatcc tgaacaagca cggttccctg ttcattcact
    29101 aattggatga tcttattcct tcgctcgaat gaattcatcg tcatttaccc atattcaatt
    29161 gtggctagtg taaacgaagg gggaccattg gtgaatgatc tgttttgcac aataccgggc
    29221 cagcatcacg ctggcccgcg gtttcatgga atgagcaaca ctttgccctg catggcatta
    29281 cgagcgatgt cacgcaccgc ctgggtgaag ctttcaaagc ttccacggtg agcgattaat
    29341 ggctccaggc ttaacttacg ttctgtcagc aaccggctcg ccgtttccca ctcctgcccc
    29401 ggccaagggc tggaatagtt catccaactg ccgataaccg tcagctcttt acgcaatatt
    29461 ttgccaaacg ttgccgatgt taaatgcaga tcctgatgca acgtgcccac cagcgccagt
    29521 tgggcatgag gaccggcaat ctctaccgcc agttcgacag tttgcggtac gccagccgtc
    29581 tcgaggataa gctgattaaa gcgcagttcg cgtaaaacgc tctgcatttg cggcgcgctc
    29641 atttcgctac tgttaaatgt ttgcatcgca ccgaaagatt ttgccagtgc cagtttttct
    29701 gagctaatgt cgatcgccgt cacactcttt gctcccagcg cgacagcgca ctgaatagcc
    29761 agcaggccaa tggttccggc accaataata ataacgtttt tattctcaca accttgcgct
    29821 aaatgaaaag catgcaggcc aacggtaatc ggctcaataa aagccccatc ctcaataggc
    29881 atatccgtgg gtagagcaaa gacatttttt cgcttaacga caatatattc agcaaatcca
    29941 ccatcacgcc gtgagccaat aaaatcatat tttgcgcact gggaataaaa ccctttcaaa
    30001 cactctggac aagtaaaaca gggtaataac ggcacacagg caaccgcatc gccagggtgt
    30061 aaatcatcaa caccggatcc caccgcatca atatagccgc taaattcatg gcctaacgtt
    30121 attggataat aatgtgcacc atttttaaat atcctgggta aatcggaacc acataagccc
    30181 gagctggcaa tttttacccg cacctcatcc tgatgtttaa tttcaggaat gacgcttttt
    30241 gcaacgcgca cgataccatc agtatcattc actactgatt tcataaaaac tcctgatt
//
LOCUS       DAEBXN010000041        29947 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.041, whole genome shotgun sequence.
ACCESSION   DAEBXN010000041 DAEBXN010000000
VERSION     DAEBXN010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29947
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.041"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            57..851
                     /locus_tag="KJL99_001916"
     CDS             57..851
                     /locus_tag="KJL99_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBD4436659.1"
                     /translation="MKIFKYTPENNLFYGYILEDMKTGFNILREIMVEGYRPSIARLY
                     DAEDGTQHFTHFADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETW
                     FNNLNWGPDKVAAERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDI
                     TMLGGHSSHSYQNGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVH
                     HHGIGKHRVHWSKLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            873..2282
                     /locus_tag="KJL99_001917"
     CDS             873..2282
                     /locus_tag="KJL99_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD4436660.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     ATLTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLCQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            2260..3039
                     /locus_tag="KJL99_001918"
     CDS             2260..3039
                     /locus_tag="KJL99_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBD4436661.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGSQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            3036..3896
                     /locus_tag="KJL99_001919"
     CDS             3036..3896
                     /locus_tag="KJL99_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBD4436662.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDAMVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSMRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     SRDPLAEARRVLVVGQGGEADNQEIVMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFMVAINHDASAAVFSQADIGVVDDWKVVLEA
                     LVTNIHAEYQ"
     gene            complement(4044..4619)
                     /locus_tag="KJL99_001920"
                     /pseudo
     CDS             complement(4044..4619)
                     /locus_tag="KJL99_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
     gene            complement(4636..4896)
                     /locus_tag="KJL99_001921"
     CDS             complement(4636..4896)
                     /locus_tag="KJL99_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HBD4436663.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQMVQRLINACPVGLFSLTQE
                     GDLRIDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            complement(4887..6158)
                     /locus_tag="KJL99_001922"
     CDS             complement(4887..6158)
                     /locus_tag="KJL99_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBD4436664.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARADLSVLLLERAEIPGSKNL
                     SGRRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWNVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRTLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGRYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWHHLRRHGLWHLAGDVIRSLRCL"
     gene            complement(6236..6598)
                     /gene="queD"
                     /locus_tag="KJL99_001923"
     CDS             complement(6236..6598)
                     /gene="queD"
                     /locus_tag="KJL99_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HBD4436665.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            6917..8716
                     /gene="cysJ"
                     /locus_tag="KJL99_001924"
     CDS             6917..8716
                     /gene="cysJ"
                     /locus_tag="KJL99_001924"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HBD4436666.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPVALAVTPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDSDVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTLIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     LETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            8716..10428
                     /gene="cysI"
                     /locus_tag="KJL99_001925"
     CDS             8716..10428
                     /gene="cysI"
                     /locus_tag="KJL99_001925"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HBD4436667.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMVKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            10502..11236
                     /gene="cysH"
                     /locus_tag="KJL99_001926"
     CDS             10502..11236
                     /gene="cysH"
                     /locus_tag="KJL99_001926"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HBD4436668.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            11500..11652
                     /locus_tag="KJL99_001927"
     CDS             11500..11652
                     /locus_tag="KJL99_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HBD4436669.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            11846..12937
                     /locus_tag="KJL99_001928"
                     /pseudo
     CDS             11846..12937
                     /locus_tag="KJL99_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311642.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
     gene            complement(13083..14120)
                     /gene="iap"
                     /locus_tag="KJL99_001929"
     CDS             complement(13083..14120)
                     /gene="iap"
                     /locus_tag="KJL99_001929"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HBD4436670.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGGCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            14372..15280
                     /gene="cysD"
                     /locus_tag="KJL99_001930"
     CDS             14372..15280
                     /gene="cysD"
                     /locus_tag="KJL99_001930"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HBD4436671.1"
                     /translation="MAQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            15282..16709
                     /gene="cysN"
                     /locus_tag="KJL99_001931"
     CDS             15282..16709
                     /gene="cysN"
                     /locus_tag="KJL99_001931"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HBD4436672.1"
                     /translation="MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGLTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEVFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            16709..17314
                     /gene="cysC"
                     /locus_tag="KJL99_001932"
     CDS             16709..17314
                     /gene="cysC"
                     /locus_tag="KJL99_001932"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HBD4436673.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLSVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            17364..17687
                     /locus_tag="KJL99_001933"
     CDS             17364..17687
                     /locus_tag="KJL99_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HBD4436674.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFLYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            17881..18192
                     /gene="ftsB"
                     /locus_tag="KJL99_001934"
     CDS             17881..18192
                     /gene="ftsB"
                     /locus_tag="KJL99_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HBD4436675.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAALQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            18211..18921
                     /gene="ispD"
                     /locus_tag="KJL99_001935"
     CDS             18211..18921
                     /gene="ispD"
                     /locus_tag="KJL99_001935"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HBD4436676.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVTRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDVQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            18921..19400
                     /gene="ispF"
                     /locus_tag="KJL99_001936"
     CDS             18921..19400
                     /gene="ispF"
                     /locus_tag="KJL99_001936"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HBD4436677.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            19397..20446
                     /gene="truD"
                     /locus_tag="KJL99_001937"
     CDS             19397..20446
                     /gene="truD"
                     /locus_tag="KJL99_001937"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HBD4436678.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            20427..21188
                     /gene="surE"
                     /locus_tag="KJL99_001938"
     CDS             20427..21188
                     /gene="surE"
                     /locus_tag="KJL99_001938"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HBD4436679.1"
                     /translation="MRILLSNYDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     QDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            21182..21808
                     /gene="pcm"
                     /locus_tag="KJL99_001939"
     CDS             21182..21808
                     /gene="pcm"
                     /locus_tag="KJL99_001939"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HBD4436680.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            21948..23087
                     /gene="nlpD"
                     /locus_tag="KJL99_001940"
     CDS             21948..23087
                     /gene="nlpD"
                     /locus_tag="KJL99_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HBD4436681.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            23150..24142
                     /gene="rpoS"
                     /locus_tag="KJL99_001941"
     CDS             23150..24142
                     /gene="rpoS"
                     /locus_tag="KJL99_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HBD4436682.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRK"
     gene            complement(24262..24669)
                     /locus_tag="KJL99_001942"
     CDS             complement(24262..24669)
                     /locus_tag="KJL99_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBD4436683.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDVADKRRRFVWLTAEGEKLLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMST"
     gene            24816..25409
                     /locus_tag="KJL99_001943"
     CDS             24816..25409
                     /locus_tag="KJL99_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HBD4436684.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            25409..26356
                     /locus_tag="KJL99_001944"
                     /pseudo
     CDS             25409..26356
                     /locus_tag="KJL99_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
     gene            complement(26629..27192)
                     /locus_tag="KJL99_001945"
     CDS             complement(26629..27192)
                     /locus_tag="KJL99_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4436685.1"
                     /translation="MRKAPQGSFPADGKEPIIERIFRLVERYPSRNAAARAWGINEGT
                     LKNYYNRRDYAPIPRPYQLKKIAESENVTLEWLMTGEGDVELKDSKEPKKENADNVDA
                     QILMFLSFLKPQEKQQVADVLGRKGAEQLLILLDEHIQELHALVGVRRAIALSLGNLP
                     DEKVREIYALSEAKGDHLNLPQKAASA"
     gene            27357..27608
                     /locus_tag="KJL99_001946"
     CDS             27357..27608
                     /locus_tag="KJL99_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBD4436686.1"
                     /translation="MRQREDSDRDWPSLKIQLALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            27610..29706
                     /locus_tag="KJL99_001947"
     CDS             27610..29706
                     /locus_tag="KJL99_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBD4436687.1"
                     /translation="MQSYVTVNDLLGVPGMPATTKGIRQALQRFSRDLGDVSRRREGT
                     KAIEYHIDCLPEITRKALRERYVEQLVATENNVSEVKAVTRKTRNPDAVQAIEAYRGS
                     PQLMEERLNALTENQRWVSEARAALVVEVLKLESAGNPGRLKAINFLVEKARKGELPE
                     RLQQAAVNANAKRGANRTISRDPLYQWVLKYNQSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQVNGRPMSEAYEDFVAEWQRRHADEPYMLEVMPSYDVVRYAMKKLPEV
                     VKQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEVV
                     GILPRLGIKHPTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRESLRVLNRD
                     LRSAFNALQQDKPLNDRQKAAMRELPSWAELIEAIREGVEWYNNRPHSELPMKPNGRH
                     YSPTEFRKKRQAEEDTEIEWLSDLELRDMFRPMVERPVRRCEIQWLNNIYYAPELRDE
                     HGRKVLISYDIHDAERITVRRKDGSFICEAIWNGNKRAAFAVSAEYHKQQQRIKGMRK
                     RAEEKIRDAEDEGIQILEHKQAEPWLSNVYRPVGNVVAVQQPEYEEEHDEEFERDFRL
                     GMQKLFAMQEEDDPLA"
BASE COUNT         7327 a         7412 c         8097 g         7111 t
ORIGIN      
        1 ttcgtcacat catcatcggc aacgaaggtg cattgtgcta tatcactgaa gtaacagtga
       61 aaatctttaa atacaccccg gaaaacaacc tcttctacgg ctatatcctg gaagacatga
      121 aaaccggctt caacatcctg cgtgaaatca tggtggaagg gtatcgtccg tcgatcgctc
      181 gtttgtatga cgctgaagat ggcacccaac acttcaccca ttttgccgac ggaaaatgtg
      241 tgctgatctt tatggctgaa ggtaaccctc gcattgcgaa ggcgacgggc gaagggattg
      301 cggaaatcgt tgcccgctac ccgcagtgcc agcgcgtgga cagcaaactg attgaaacct
      361 ggtttaacaa cctgaactgg ggaccggata aagtggctgc cgaacgtgtg cagatcctca
      421 aaaccggcaa catgggcttt accaccgaag tgtccggctg ctggagctgc atccacgaaa
      481 tctacgaaag cgttattaac cgtattcgta ctgagttccc gcacgccgac gacatcacca
      541 tgctgggcgg tcactcctct catagctatc agaacggcac caacatgtac ttcgtctatg
      601 actacaacgt tgttgactgt aagccggaag aggaaatcga caagtaccac aatccgctca
      661 acaaaatcat ctgcgaagaa accattcgcc tcggcggttc gatggtgcac caccatggca
      721 ttggtaaaca tcgcgttcac tggagcaagc tggaacacgg cagcgcgtgg gcgttgctgg
      781 aagggctgaa aaagcagttc gatcctaacg gcattatgaa tacgggtact atctatccga
      841 ttgaaaaata atgtatcagg cagcgttccg cgatgacggg gcgttgcctt ttcggcttct
      901 caggcgagaa gccgttctta ttaccggaca atgaaggggt aaagatgaac acttcaccgg
      961 tgcgaatgga tgatttaccg cttaaccgtt ttcactgtcg aattgctacg ctcactttcg
     1021 gcgcacacct gaccgacggt tatgttctag gcgtcattgg ttacgccatt attcaactta
     1081 cgcccgccat gcaactgacg ccgtttatgg cgggaatgat cggcggctcg gcgctccttg
     1141 gtttgtttct tggcagcctg gttcttgggt ggatctccga ccatattggt cggcaaaaaa
     1201 tcttcacctt cagctttttg ctgattacgc tcgcttcgtt cttgcaattt tttgccacca
     1261 cgccagagca tcttattggg ctgcgcattt tgatcggcat tggtctggga ggcgattact
     1321 cagtaggtca caccttgctg gctgaatttt ccccgcgccg ccatcgcggt attttgctgg
     1381 gcgcattcag cgtggtgtgg accgtaggct atgtgttggc aagtattgcc ggacatcact
     1441 ttatttccga aaacccggag gcctggcgct ggttgctggc atcggcagct ctgcccgcgt
     1501 tgttgattac gttattacgc tggggaacgc cagaatcgcc acgttggcta ctgtgccagg
     1561 ggcgttttgc agaagctcac gctatcgtgc atcgctattt tggtccccat gttttactgg
     1621 gcgatgaagt ggtaacggcg acccataaac acatcaaaac cttgttctct tcgcgttact
     1681 ggcggcgcac ggcgtttaac agcgtcttct ttgtctgcct cgtaatccca tggtttgtga
     1741 tttatacctg gctgccaact atcgcccaga ctattggtct ggaagatgcg ctgactgcca
     1801 gcctgatgct taatgcgttg ttaattgtgg gcgcgctgct gggattagtt ctgacgcacc
     1861 tgctggcaca tcgcaaattt ttgctgggaa gttttttgct gctggcggca acgctggtag
     1921 taatggcctg tttgccttcc ggcagttcat taacgctgct gctttttgtt ctcttcagca
     1981 ccaccatttc ggcagtcagt aatctggtgg gcattttgcc tgcggaaagt tttcctactg
     2041 acattcgctc gctgggagtc ggttttgcca ccgccatgag tcgacttggc gcggcggtaa
     2101 gtactggcct gctgccgtgg gtgctggcgc agtggggaat gcaagtcacc ttattgctcc
     2161 tagcgacagt gttgttggtt ggttttgttg tgacctggct atgggcacca gaaactaaag
     2221 ccctcccgct ggtggcggcg ggaaatgtag gaggtgcgaa tgaacattct gttagcgttt
     2281 aaagccgaac cggatgccgg aatgctggcg gaaaaagagt ggcaggcggc ggctcagggt
     2341 aatagcggac cggatgtttc gctactgcga agtttactcg gtgctgatga acaggccgct
     2401 gccgcgctgc tgttagcgca gagaaaaaac ggtacgccga tgtctttaac cgcgttgagc
     2461 atgggggatg aacgggcgtt gcactggctg cgctatctca tggctcttgg gtttgaggaa
     2521 gctgtattgc tggagacggc ggcagatctg cgctttgcgc cggaatttgt cgctcgccat
     2581 attgccgaat ggcagcgtca gaatccgctg gatctgataa ttaccggcag ccaaagcagc
     2641 gaagggcaga atgggcaaac gccatttttg ctggcggaaa tgctgggctg gccctgcttc
     2701 acccaggtgg agcgtttcac tctcgacgcg ctgtttatca ccctcgaaca gcgtactgaa
     2761 catgggttgc gctgttgccg ggttcgcttg cctgcggtga ttgccgtgcg tcagtgcggt
     2821 gaagtggcct tgccagtacc gggaatgcgc cagcgaatgg ctgcggggaa agcagaaatt
     2881 atccgtaaaa ctgttgctgc cgaaatgcca gcgatgcagt gtctgcaact ggcaagagca
     2941 gagcaacgac gcggagcgac gctgattgat gggcaaacgg tagcagaaaa agctcaaaag
     3001 ctatggcagg actatttacg ccaaaggatg cagccatgaa tatcgcaatt gtgacaataa
     3061 atcaggagaa tgccgcaata gcgagctggc tggcggcgca ggatttcagc ggttgtacgc
     3121 tggcgcactg gcaaatagaa cctcagcccg tagtggcgga acaggtgctg gatgcgatgg
     3181 tggaacagtg gcagcgaacg cctgcggacg ttgttctctt tccgccggga acgtttggcg
     3241 atgaactgtc gacccggctg gcctggcgtt tacatggtgc aagcatctgt caggtgactt
     3301 cgttagatat tccaaccgta agcatgcgca aatcccactg gggaaatgcg ttaacggcga
     3361 cgctgcaaac agaaaaacgc ccgttatgcc tttccctggc gcgtcaggcg ggagcagcga
     3421 aaaacgccac gttgccctct ggaatgcagc aactgaacat tgtcccaggt gcgcttcccg
     3481 actggctggt tagcacagag gatctgaaaa atgttagccg cgacccatta gctgaggcca
     3541 gacgggtgct ggtggtggga cagggcggag aggcagataa ccaggaaatc gttatgctgg
     3601 cagaaaagct gggagccgaa gtgggctaca gtcgggcgcg agtgatgaac ggtggcgttg
     3661 atgctgaaaa agtgattggt atttccggac atctgctggc acctgaggtg tgcattgttg
     3721 ttggcgcttc cggcgcagca gcgttaatgg cgggcgtacg caatagcaag tttatggtgg
     3781 cgattaatca tgacgccagc gcagcggtat tttcacaggc agatattggc gtggttgatg
     3841 actggaaagt ggtgctggag gcactggtga caaacattca cgcagaatac caataacgtg
     3901 ctgccggatg atcctgccta cggttctgga ctggggacct gtaggcctga taagacgcgt
     3961 taagcgtcgc atcaggcgct gaatgtcgga tgtggcgtgc ggccaacaaa tcaaaacaaa
     4021 ttcaattggt tacgattatc ccgtcaaagc aattttttcg ctaacgtcca gactccggta
     4081 ttcgtggtgg aaagcgccac gacacccgcg ttaatcgcat tacgcgcatc ttcttcatcg
     4141 cataccagcc cgccagcaat cagcggttgg cggatttttt ccgtcaccca gcccagcact
     4201 ttgggcatgc agcctggcag gatctcaata caatccggat tcgattgcgc aacttactta
     4261 tcaatgttgt gaaacgaaat cgaatcaaca ataaacaggt gatgaataca gaaaaaaact
     4321 tccgctcttg ccgcttttag catcgatgct ttggtgctga taattccatc cgcctcggtc
     4381 accagtttca aaaactgaat caccacttct ttatttgacg cgccttccag caaatcaaca
     4441 tgaataaaag catatttccc ggcgttctta atctttttaa cgatattact gatggtgcag
     4501 atattgccgt acaacacgga aataaactgg cattcagaat cgatggccag ttgcaggctg
     4561 gcattatctt taacggcagc aatcaccgga ttctggcgga gcaggtgtaa gaggggcata
     4621 aattatcctt attatttatc caaagcggta ggtgatgccg aatccggaag gcggatagcg
     4681 ccactgttgt agtgtggatt cgtcgcacag caaacggcag gtgccacact ccaggcagcc
     4741 gcgatagtca atacgtaagt caccttcctg tgtgagagaa aaaagaccta ccggacaggc
     4801 gttaatcaac cgttgtacca tctgacgctc aacggagtca gccggaacaa tgtgcggcgc
     4861 atcagcaacg cgccagagat tacgggctac agacatcgca gactcctgat aacatcgccc
     4921 gccagatgcc acaggccatg acgacgtaaa tgatgccaga ataactggcg cagcggtgga
     4981 acaggtttat caccctgatc ccataaatcg cgggaaatat cctgcattaa cgcaggccac
     5041 gcccgatacc tgccagggcg ttgcaaaagc gccggaacat gctgataacg ttgtagaaca
     5101 tcccatagca ggctgcgctc tacgttgtga tgataaagcg caaacagatt ttgcggctcg
     5161 cggtgctggc aggcgcttat tagcgtttgt gccgccgcct gtgcgccagt cagcgccata
     5221 tccatgccgc gcacggaaat tccggtattg acgcaactgc gcaacgcatc gcccaccagc
     5281 agccagccgt taccggcgta ttgcaccggc atactgtgca agccaccttc tggcaccaga
     5341 tgcgcaccat actccagtga ttccgtgttt ttgataagcg tgcgcactgc cggatgcgct
     5401 ttaaagcgag tcagcagctc gcttgccgga acacgacttt gcgtaaggga agagagcggg
     5461 caaacaatcc ctaacgagag cgtttgttga ttagtataaa gaaatgcgcc gccgggtaag
     5521 tcatcacaga ttccgccgct gaacagcaac gctgcgcctt cgttattctc cagatgaaaa
     5581 cgttcttcaa tagcggatgt ttccagcgac agcacttctt tgatccccaa cgccatcgct
     5641 tcgccagcag gacgagtcac taacccgtga cgttcagcca ggacgctgtt ggcaccttct
     5701 gccagcacca cataacgggc gcggagaata tcgtcaccac aaataacgcc acagactctg
     5761 ccgttttctt catacagtgc atccaccgtc gctccgggga tgcattcgac accttctttt
     5821 tcggcttcgg caaccagcca cggatcgaac cgtgcacgta atacattcca ggattcaccg
     5881 ccgggctgta agctggaaaa cgtcgttgcg ccatccggcg ttaacaggga aaggctttcg
     5941 tgagtgatgc gtcgttcaag aggcgcggtc agatgaaatt gcgggaggag ttccgcgagt
     6001 gcatgggtat ataaccgcct gccggaaaga tttttgctgc cggggatttc agcgcgttcg
     6061 agtaacaaaa cggataaatc cgctcgcgcg cagcgtaacg cgcaagcggt gcctgcaata
     6121 ccggcaccaa taataataat gtcgcagtcg tcttccatgt catgccccag atatccagaa
     6181 cacaacaccc taacatagcg ttactaaagg gaaattgacc gccgacacac tcttatcatt
     6241 cgccgcgata gatacaccct gcggtgcagg tttcttttac catcaccgca cttaacagcg
     6301 gcacaacggg tttaacctga tcccaaatcc attttgctaa aacttcgctg gttggatttt
     6361 ccagacctgg aatatcattg agataatggt gatcgaggcg ctcataagtt ggcttaaacg
     6421 ccgctttcag ttcagcgaaa tcgataatcc agcccgtatg cggatcgact tccccggtaa
     6481 tttccagtcg caccataaag gaatgcccgt gcaggcgacc acatttatgc ccttccggga
     6541 catgtggtaa gcggtgagcg gcttcgaagg tgaaatcttt aaataacgtg gtggacatca
     6601 taatttctct acaggacgat aaaaccgccg tagagtaccg gaaaggctgt tttttcgcat
     6661 tatctaaaac gactttgttg cgcaaaatcg ctgatttatc ttaatgattg gctaaattca
     6721 tttgtttttc attaggttgg ttaatctatt ttgttgttaa agactattgc taaaacaggt
     6781 tagtcgattt ggttattagt tatcgctatc ccgtctttaa tccacaccgt tcaccccgtt
     6841 aaccttacct tctcttctgt tttatgggcg ctgacagggc gcagaaacag ctttgcttac
     6901 tggaacataa cgacgcatga cgacacaggt cccaccttcc gcgttgcttc cgttgaaccc
     6961 ggagcaactg gcacgccttc aggcggccac gaccgattta acgcctaccc agcttgcctg
     7021 ggtttctggc tatttctggg gcgtgctcaa ccagcagcct gttgcgcttg cagtgacgcc
     7081 agcgcccgcc gcagaaatgc cgggtataac gattatctct gcttcgcaaa ccggcaatgc
     7141 gcgccgggtt gctgaagcat tacgcgatga tttattagca gcaaaactga acgttaagct
     7201 ggtgaacgcg ggcgactata aattcaaaca aatcgccagc gaaaaactgc tcatcgtagt
     7261 gacgtcaacg caaggggaag gggaaccgcc ggaagaagcc gtcgcgctgc ataagttcct
     7321 gttctccaaa aaagcgccaa agctggaaaa caccgcgttt gccgtgttta gcctcggcga
     7381 tagctcttat gaatttttct gccagtccgg gaaagatttt gacagcaagc tggcggaact
     7441 ggggggtgaa cgcctgctcg accgtgtcga ttctgatgtt gaataccagg ctgctgccag
     7501 cgagtggcgc gcccgcgtgg ttgatgcgct taaatcgcgt gcgcctgtcg cggcaccttc
     7561 gcaatccgtc gctactggcg cggtaaatga aatccacacc agcccgtaca gcaaagacgc
     7621 gccgctggtg gctagcctct ctgttaacca gaaaattact gggcgtaact ctgaaaaaga
     7681 cgttcgccat atcgaaattg acttaggcga ctcgggcctg cgttaccagc cgggtgacgc
     7741 gctgggcgtc tggtatcaga acgatccggc actggtgaaa gaacttgtcg aactgctgtg
     7801 gctgacaggc gatgaacctg tcactgtcga gggcaaaacg ttgcctctga acgaagcgct
     7861 acagtggcac ttcgaactga ccgtcaacac cgccaacatt gttgagaatt acgccacgct
     7921 tacccgcagt gaaacactgc tgccgctggt gggcgataaa gcgaagttac agcattacgc
     7981 cgcgacgacg ctgattgttg acatggtgcg tttctccccg gcacagcttg atgccgaagc
     8041 gctaattaat ctgctgcgcc cgctgacccc gcgtctctat tccattgcct cctcgcaggc
     8101 ggaagtcgag aacgaagtac acgtcaccgt tggtgtggtg cgttacgacg tggaaggccg
     8161 cgcccgtgcc ggtggtgcct ccagcttcct cgctgaccgc gtggaagaag agggcgaagt
     8221 ccgcgtattt atcgaacata acgataactt ccgcctgccc gctaacctgg aaaccccggt
     8281 gattatgatt ggcccaggca ccggtattgc gccgttccgc gcctttatgc agcaacgcgc
     8341 cgctgacgaa gcgccaggta aaaactggct gttctttggc aatccgcatt ttacggaaga
     8401 cttcctgtat caggtggagt ggcagcgcta cgtcaaagag ggtgtgctga cgcggatcga
     8461 tctcgcctgg tcgcgcgacc aaaaagaaaa agtttacgta caagataaac tgcgcgaaca
     8521 gggcgcggag ctgtggcgct ggatcaatga cggtgcccac atttatgtct gcggcgacgc
     8581 taatcgcatg gcgaaagacg ttgagcaggc acttctggaa gtgattgccg aatttggtgg
     8641 catggacacc gaagcggcgg atgaattttt aagtgagctg cgcgtagagc gccgttatca
     8701 gcgagatgtc tactaatgag cgaaaaacat ccagggcctt tagtggtcga aggaaaactg
     8761 acagacgccg agcgcatgaa gcttgaaagc aactacctgc gcggcaccat tgcggaagat
     8821 ttaaacgacg gtctgaccgg cggctttaag ggcgataact tcctgctgat ccgcttccac
     8881 ggcatgtatc agcaggatga ccgcgacatc cgcgccgaac gtgctgaaca gaagctggag
     8941 ccgcgccacg cgatgctgtt gcgctgccgt ctgccgggtg gagttatcac caccaaacag
     9001 tggcaggcga tcgataaatt tgccggtgaa aacaccatct atggcagcat tcgcctgacc
     9061 aaccgccaga cgtttcagtt ccacggcatt ctgaaaaaga acgtcaaacc ggtgcaccag
     9121 atgctgcact cggtcggtct tgatgcgctg gcgactgcta acgacatgaa ccgtaacgta
     9181 ctctgcacct cgaaccctta cgagtcgcag ctgcacgcag aagcgtacga gtgggcgaag
     9241 aagatttctg aacatctgct gccgcgtacc cgcgcgtatg cggagatctg gctcgatcag
     9301 gaaaaagtcg ccaccaccga tgaagaaccg atccttggtc agacttacct gccgcgtaag
     9361 ttcaaaacca cggtagtgat cccgccgcag aacgatattg atctgcacgc caacgacatg
     9421 aacttcgtgg cgatcgcgga aaacggcaag ctggtgggct tcaacctgct ggtgggcggt
     9481 gggctttcta tcgaacacgg taacaagaaa acctacgccc gcacggcgag tgagtttggc
     9541 tatctgccgc tagagcatac gctagcggtg gcggaagccg tcgtgacaac tcagcgtgac
     9601 tggggtaacc gaaccgatcg taaaaatgcc aaaaccaaat acacactgga gcgcgtgggg
     9661 gttgagacgt ttaaagcgga agtggagcgt cgcgcgggga tcaaatttga accgatccgc
     9721 ccgtatgaat ttacaggacg cggcgatcgt attggctggg ttaagggcat tgatgataac
     9781 tggcacctga cgctgtttat cgaaaatggt cgcatccttg attatccggg gcgtccgctg
     9841 aaaactggcc tgctggagat cgcgaagatc cacaaaggtg atttccgcat tacggcgaac
     9901 cagaatctga tcatcgccgg tgtgccggaa agcgagaaag cgaagatcga gaaaattgcc
     9961 aaagagagcg ggttaatgaa tgccgtcacg ccgcagcgtg aaaactcgat ggcctgcgtg
    10021 tcattcccga cttgcccgct ggcgatggcg gaagcggagc gtttcctgcc gtcttttatc
    10081 gacaacatcg ataatttaat ggtgaaacat ggtgtcagcg atgagcatat cgtgatgcgt
    10141 gtaacaggct gcccgaacgg ttgtggtcgc gcgatgctgg cggaagtggg cctggtgggt
    10201 aaagcaccgg gtcgctacaa cctgcatctt ggcggcaacc gcattgggac acgtatccca
    10261 cggatgtata aagaaaacat caccgagccg gaaatcctgg cgtcgcttga tgaactgata
    10321 gggcgctggg cgaaagagcg cgaagcgggt gaaggcttcg gcgactttac ggtgcgtgcg
    10381 ggcatcattc gcccggtgct cgatccggcg cgcgatttgt gggattaacc atcagcccgg
    10441 tcttgtaggc ctgataagac gcgtgagcgt cgcatcaggc aaggcaaaca gtgaggaatc
    10501 tatgtccaaa ctcgatctaa acgccctgaa cgaactgccg aaggtagatc gcattctggc
    10561 gctggcagaa actaacgccg aactggaaaa actggacgct gaaggccgcg ttgcctgggc
    10621 gctggataat ctgcccggtg aatatgtact ttcttccagc tttggcattc aggcggcggt
    10681 gagcctgcat ctggtgaatc agattcaccc ggatattccg gtgatcctca ccgacaccgg
    10741 ctatctgttc ccggaaacct accgctttat tgacgagtta acggacaaac tcaagctcaa
    10801 cctgaaagtg taccgcgcta ccgaaagcgc cgcctggcag gaagcacgct acggcaaact
    10861 gtgggaacag ggcgttgaag gcattgaaaa gtacaatgac atcaacaaag tcgaaccgat
    10921 gaaccgggcg ctgaaagaac tgaatgcgca aacgtggttt gctggcctgc ggcgtgaaca
    10981 atccggcagc cgtgccaatt taccggtgct ggcaattcag cgtggcgtat ttaaagtgct
    11041 gccgattatc gactgggata accgaactat ttatcagtac ctgcaaaaac atggcctgaa
    11101 atatcaccca ttatgggacg aaggatattt atcggtgggc gatacccata caacccgtaa
    11161 atgggaaccc ggcatggcgg aagaagaaac gcgtttcttt ggcttaaaaa gggaatgtgg
    11221 attacacgaa gggtaaatat tgccggataa attaaacgcc tctgtcagaa atgatggggg
    11281 cgtttttatt ttgcgagcag cattccggca tttaaaggtg aacatcgcag ggaaaattaa
    11341 caggtataga atggcgttgg gtgcttgagg ctgtctggcg ttaagcattc gcgaggttcc
    11401 agatggacaa aagccccagg cgatatttct atcaacctga ggctcgcgtt cgaacgtagc
    11461 aattcggatg ttagtctctt ctttgcaggg aggcaagaca tgctgacaaa atatgccctt
    11521 gtggcaatca tcgtactgtg ttgtacagta ctgggattca cgttgatggt aggtgactcg
    11581 ttatgtgagt tgagtatcag agaacgtggt atggagttta aggcagttct cgcttacgaa
    11641 tcgaagaagt agccaccacg cgggggtaaa accccgcgta ctgaattgtg atggtttggt
    11701 ctcctgcacc ctttttttaa atttatattt tttatgtgaa tatatagtct ctatttatta
    11761 ttatgaattt caaatgaaaa tcattaattt attcataatg taaatgtttt attggctgat
    11821 atatatcata gatagttttt aaaatatgcg taaatatcct ttaagtttac tgaaggataa
    11881 aaatattgtg actttctttg atttctgggg aaaaaaccga cgtggcgaga aagagggtat
    11941 cgacgactat caccttcttt gctggcattt gctggatgtg gccgcaatgg gctatttaat
    12001 ggttaaaaga aattgcttcg ggctggctga ttattttcgt caattaggga tttctgacaa
    12061 ggaacaggca gctcgatttt tcgcctggtt gctgtgctgg cacgatattg gaaaatttgc
    12121 ccgctctttt caacaacttt acctgccccc tgaattcaag attcaggaag gtgccagaaa
    12181 aaattacgaa aaaatctctc attcaacgct gggctactgg ttgtggaatc attatttaag
    12241 tgaatgtcag gagttacttc cttcatcttc gctctctccg cgcaaactta ggcgagtgat
    12301 agaaatgtgg atgcccgtga cgacagggca tcatggtcgg ccccctgatc gtatggatga
    12361 attggataat tttctgcctg aagacaaagc tgcagcacga gattttctcc ttgaaatcaa
    12421 gccactgttt ccgcgaatag agatccccac attctgggat gatgacgagg gcgttgaact
    12481 tataaaacaa ctttcctggt atatctctgc aaccgtcgta cttgcagact ggacagggtc
    12541 gtcaacgcga ttttttccac gcgtcgcaca ggcaatggat attgaagatt actggcaaaa
    12601 ggctttagtt caggctcaaa acgcattaac cgtctttccg ccacaagcag aaaccgcgcc
    12661 tttcaccgga attaatacgc tgtttccttt tattgagaat acgacaccgt tacagcaaaa
    12721 ggtgctggat ctggatatca atcagccagg accacagttg tttattctgg aagatgtgac
    12781 cggcgcaggt aaaacagaag cggcgcttat cctggcgcac aggttgatgg ctgcgggtaa
    12841 cgctcagggg ctgttttttg gcctgccaac aatggcaacg gccaatgcca tgtacgatcg
    12901 actggtcaaa acctggcttg ctttctattc gccagaggtg ttccccgctc cagcggagat
    12961 aaaccggcgc aatacggtgc ggttttcccg gaagtaaacc ggacgcactg gatgcgatga
    13021 tgggtatcac ttagaattcc ccgcccctgc ggtagaactc ccagctccca ttttcaaacc
    13081 catcaagacg ccttcgccag ctccttcacc aacggtagca ttatccgcat aacgtcacgg
    13141 cagcgacgtt ctattcttcc aggaagtgcc ttatcaatat gttgttgatt atccagtctt
    13201 acgtcatgcc agctatttcc cgccgggaag gcaggtgttt ttgcgcgttg ctgataacca
    13261 tccttatttc caagattcca gttagtcgct tccaccgaaa gtacagcaat gcccgctttg
    13321 tcgaatattt ctgcgtcatt acaaccccca gtgcctttcg gataattttt attcaaaccc
    13381 ggattggtcg ttgcggctat tccgtgactg cgtgcaattg ccagcgccct gtcgcgcgtt
    13441 aatttcctta ctgcttcagg ggtttttaca ccgctgttga aatacaattt atcgccaaca
    13501 attaagttat cgagattaat caccagcagc gtatttttct tttcggtgtc actcatccgc
    13561 ttgagtaaat tctcagcgcc taattttcct tcctcttcgc cgctggtcgc cacaaatcga
    13621 ataccatact cggtaggcgt atttttcagg cgttctgcca gttccagcat gacacctaaa
    13681 cctgcggcgt tatcatctat tccttgtaac gtcagtccgc cgagattggc atctgcgtca
    13741 gcatcgctca gcggggcgta agtatccaga tgcgccataa tgatgatctg ctgcggcgct
    13801 ttgccttcat gagcggcaat caccgtactt cctgtcacgt tatgccagtt cttgcgatta
    13861 tcgcgggcgg tataaatata ccgactatta aatgtccgaa tatcactgcg ataacccatt
    13921 tgctgaaact gttggcgaat ataatcggca gataacattt ctgctggagt tccggtcatg
    13981 cgtcccggaa agaaagtagc aatatgtcgt gcctgggtat tagcaaaatc gccaggttta
    14041 ggtgacgagg cgtgtacggg gagaataaag catacgccga gcgccagggc agcggtacgg
    14101 tggcgcaatg cggaaaacat agtgagtcct taaataccat gcaaattttt ttaccgccat
    14161 agtatgaaac tgccgctgcg ctaaaacaat ttcaaatctt cctaaacgcc cgaaatccgg
    14221 tgccttaagc actttttgat attagctttg ccaaatcgtt attccgttaa ggaactactc
    14281 attctaattg gtaatttcat tcgttctctt acgctcccta tagtcgaaac atctgatggc
    14341 aagaaaatag cggtattgca aaggaacggt tatggctcaa aaacgactta ctcacctgcg
    14401 gcaactggag gcggaaagca tccacattat tcgcgaggtg gcggcagaat tctcaaatcc
    14461 ggtgatgctc tactctatcg gtaaagattc cagcgtcatg ctgcatctgg cgcgcaaggc
    14521 gttttatcca ggtacgctgc ctttcccgtt gctgcatgtc gataccggct ggaaattccg
    14581 cgagatgtat gagttccgcg atcgtacggc gaaagcctac ggctgcgagc tgctggtgca
    14641 taaaaacccg gaaggcgtgg cgatggggat taatccattc gtgcacggca gcgcgaaaca
    14701 taccgatatt atgaaaactg aaggcctgaa acaggcgctg aacaaatacg gttttgatgc
    14761 cgccttcggt ggtgcgcgcc gtgacgaaga gaaatcccgc gctaaagagc gaatctactc
    14821 tttccgtgac cgcttccatc gctgggatcc gaaaaatcag cgcccggagc tgtggcacaa
    14881 ctacaacggg caaattaaca aaggcgaaag catccgcgtc ttcccgctct ctaactggac
    14941 cgagcaggat atctggcaat acatctggct ggaaaatatc gacattgtgc cgctgtatct
    15001 cgctgcggaa cgtccggttc tggaacgcga cggtatgttg atgatgattg atgacaaccg
    15061 tatcgacctg caaccgggcg aagtgatcaa aaaacggatg gtgcgtttcc gtacgctggg
    15121 ctgctggccg ctgaccggtg cggtggagtc aaatgcacaa acactgccgg aaatcatcga
    15181 agagatgctg gtttccacca ccagtgaacg tcagggtcgc gtgattgacc gcgaccaggc
    15241 gggatccatg gagctgaaaa aacgtcaggg gtatttttaa gatgaacacc gtacttgcac
    15301 aacaaatcgc caatgaaggc ggcgtcgaag cctggatgat tgcgcaacaa cataaaagcc
    15361 tgctgcgttt tctgacctgt ggtagcgtcg atgacggcaa aagtaccctg attggtcgtc
    15421 tgctgcacga tacccgccaa atctacgaag atcagctctc atcgctgcat aacgacagta
    15481 agcgtcacgg cacccagggc gaaaagctgg atctggctct gctggtggac ggcctgcaag
    15541 ctgagcgcga acagggcatc actattgatg tggcctaccg ctatttctct accgagaagc
    15601 gtaaatttat tatcgccgac accccagggc acgagcagta cactcgcaat atggcgactg
    15661 gcgcatcgac atgtgaactg gcgatcttac tgattgatgc ccgtaaaggc gtgctcgatc
    15721 aaacccgtcg tcacagtttt atctccacac tgttggggat caaacatctg gtcgtggcga
    15781 tcaacaaaat ggatctggtg gattacagtg aagagacgtt cacccgtatt cgtgaagatt
    15841 atctgacctt tgccgggcag ctgccgggta atctggatat ccgctttgtg ccgctctccg
    15901 cactggaagg tgacaacgtg gcttcgcaaa gtgaaagtat gccgtggtac agcggtctga
    15961 cactgctcga agtgctggaa accgtggaga tccagcgagt ggtggatgcc cagccaatgc
    16021 gcttcccggt gcagtacgtt aaccgcccaa atctcgattt tcgtggctac gccggaatgc
    16081 tggcatccgg tcgcgtggaa gtcgggcaac gtgtaaaagt gctgccctct ggtgtggaat
    16141 caaacgtcgc gcggatcgtg acttttgatg gtgatcgcga agaagtcttt gccggagaag
    16201 cgatcaccct ggtgctgacg gatgagatcg atatcagccg tggtgatctg ctgctggcgg
    16261 cagacgaagc gttaccggcg gtgcagagtg cgtcggtgga tgtggtatgg atggcggaac
    16321 agccgctttc cccgggccag agttatgaca tcaaaattgc cggtaagaag acgcgtgctc
    16381 gtgttgatgg cattcgttat caggttgata ttaataacct tacccaacgc gaagttgaaa
    16441 acctgccgct gaacggcatc ggcctggtgg atctcacttt tgacgagcca ctggtgttag
    16501 atcgctatca gcaaaatccg gttaccggtg ggctgatttt tatcgatcgc ctgagcaatg
    16561 tgaccgtggg tgccggtatg gtgcacgagc cagttagcca ggcaacagct gcgtcatctg
    16621 aattcagtgc attcgaactg gaattgaatg ccctggttcg tcgccacttt ccgcactggg
    16681 gcgcgcgcga tttgctgggg gataaataat ggcgctgcat gacgaaaacg tcgtctggca
    16741 tagtcatccg gtcactgtgc aacaacgcga actacaccac ggtcatcgtg gtgtagtgct
    16801 gtggtttacc ggcctctccg ggtccggtaa atcaacggtc gccggggcgc tggaggaggc
    16861 gttacataaa ctcagcgtca gtacgtatct gctggatggc gacaatgttc gccacggatt
    16921 atgcagcgat ctcggtttta gcgatgccga tcgtaaagag aatatccgtc gcgtcggtga
    16981 agtggcgaat ttgatggttg aagccggact ggtggtgctg accgcattta tctcgccaca
    17041 ccgcgccgaa cgccagatgg ttcgcgaacg cgtaggagaa gggcgcttta ttgaagtgtt
    17101 tgtcgatacg ccgctggcga tttgcgaagc ccgcgatcca aaaggcttat ataagaaagc
    17161 gcgtgccggt gaactgcgca actttacggg aatagattcc gtttacgaag cgcctgaatc
    17221 ggcagaaatt catctcaatg gtgaacaatt agtaacaaat ttggtacagc aattattaga
    17281 tctgttgaga cagaacgata ttatcagatc ctgagacacc accgggtttc catgcccggt
    17341 tcgtcaaagt aacaggatca aatatgcgta atagccataa cattacacta acaaataacg
    17401 acagccttac tgaggatgaa gaaactacat ggtcactgcc tggtgccgtg gtcggtttta
    17461 tctcctggtt atttgcgctg gcgatgccga tgttgattta tggctctaac acgctgttct
    17521 tctttctcta cacctggcct ttctttctgg cactgatgcc tgtcgcggta gtggtgggga
    17581 ttgcgctgca ttcattgatg gacggaaagc tacgctacag tattgttttc accctggtga
    17641 cagtaggcat tatgtttggc gcactgttta tgtggctact gggctaattt gtactttctt
    17701 gtctcctctg ttcataattc aaaccgtaac taataatgag attatgttct gcacgccctg
    17761 ggtatacgta acaatgggca aatgtggtac atttgcccgc gttgtcgcgg tatccccaac
    17821 agaggatgta gagtcgtctt cggatgcatg ggatgatgat gcagtttttc agggggcagg
    17881 atgggtaaac taacgctgct gttgctggct attctggtct ggctacagta ttcgctgtgg
    17941 ttcggtaaga acggtataca tgactatacc cgcgtcaatg atgatgtggc ggcactgcaa
    18001 gctacaaacg cgaaacttaa agcgcgaaac gatcaacttt ttgccgaaat tgacgatctc
    18061 aatggcggcc aggaggcgct cgaagagcgt gcgcgtaatg aactcagcat gaccaggccg
    18121 ggcgaaactt tttatcgtct ggtgcctgac gcgtcgaagc gcgcacagtc tgcggggcaa
    18181 aacaatcgat aaatcagccc gggaattaac atggcaacca ctcatttgga tgtttgcgcc
    18241 gtggttccgg cggccggatt tggccgtcga atgcaaacgg aatgtcctaa gcaatatctc
    18301 tcaatcggta atcaaaccat tcttgaacac tcggtgcatg cgctgctggc gcatccccgg
    18361 gtgacacgtg tcgtcattgc cataagtcct ggcgatagcc gttttgcaca acttcctctg
    18421 gcgaatcatc cgcaaatcac cgttgtagat ggcggtgatg agcgtgccga ttccgtgctg
    18481 gcaggtctga aagccgctgg cgatgtgcag tgggtattgg tgcatgacgc cgctcgtcct
    18541 tgtttgcatc aggatgacct tgcgcgattg ttggcgttga gcgaaaccag ccgcacggga
    18601 gggatcctcg ccgcaccagt gcgcgatact atgaaacgtg ccgaaccggg caaaaatgcc
    18661 attgctcata ccgttgatcg caacggctta tggcacgcgc tgacgccgca atttttccct
    18721 cgtgagctgt tacatgactg tctgacgcgc gctctaaatg aaggcgctac cattaccgac
    18781 gaagcctcgg cgctggaata ttgcggattt catccgcagt tggtcgaagg ccgcgcggat
    18841 aacattaaag tcacgcgccc ggaagatttg gcactggccg agttttacct cacccgaacc
    18901 atccatcagg agaatacata atgcgaattg gacacggttt tgacgtacac gcctttggcg
    18961 gtgaaggccc aattatcatt ggtggcgtac gcattcctta cgaaaaagga ttgctggcgc
    19021 attctgatgg cgacgtggcg cttcatgcgt tgaccgatgc attgcttggc gcggcggcgc
    19081 tgggggatat cggcaaactg ttcccggata ccgatccggc atttaaaggt gccgacagcc
    19141 gcgagctgct acgcgaagcc tggcgtcgta ttcaggcgaa gggttatacc cttggcaacg
    19201 tcgatgtcac tatcatcgct caggcaccga agatgttgcc gcacattcca caaatgcgcg
    19261 tgtttattgc cgaagatctc ggctgccata tggatgatgt taacgtgaaa gccactacta
    19321 cggaaaaact tggatttacc ggacgtgggg aagggattgc ctgtgaagcg gtggcgctac
    19381 tcattaaggc aacaaaatga ttgagtttga taatctcact tacctccacg gtaaaccgca
    19441 aggcaccggg ctgctgaaag ccaatccgga agactttgtg gtggtggaag atttgggctt
    19501 tgagcctgat ggtgaaggtg agcatattct ggttagaatc ctcaaaaacg gctgcaatac
    19561 ccgttttgtg gcggatgcac tggcgaaatt cctgaaaatt cattcccgtg aagtcagctt
    19621 cgctgggcaa aaagacaaac atgctgtaac ggaacagtgg ttatgtgctc gcgtgccggg
    19681 caaggaaatg cccgatctga gcgcctttca actggaaggc tgccaggtgc tggagtatgc
    19741 gcggcacaag cgcaagctgc gtttaggggc gctgaaaggt aacgccttta ccctggttct
    19801 gcgcgaagtg agcaatcgcg atgacgttga acaacgtctg atcgatattt gcgtaaaagg
    19861 tgtaccgaac tacttcggtg cccaacgttt tgggattggc ggtagcaact tgcagggcgc
    19921 gctgcgctgg gcgcaaacca atactccggt gcgcgatcgc aataaacgga gtttttggtt
    19981 gtcggcagcc cgcagtgcgt tgtttaatca gattgttgct gagcgcctca aaaaagcaga
    20041 cgttaatcaa gttgttgacg gcgatgcgct acaattagcc ggacgtggta gctggtttgt
    20101 agcaaccacc gaagaactgg cggaattaca gcgtcgcgtc aacgataaag agctgatgat
    20161 aaccgccgca ttgccgggca gtggcgaatg gggaactcag cgtgaagcgc tggcattcga
    20221 acaagcagct gtcgccgcag aaactgaatt acaagcttta ctggtgcgcg aaaaagttga
    20281 agccgcgcgc agagcgatgc tgctgtatcc gcaacaatta agctggaact ggtgggatga
    20341 cgtcaccgta gagatccgtt tctggcttcc ggcgggtagt tttgcaacca gcgttgtcag
    20401 ggaacttatc aacacaacag gtgattatgc gcatattgct gagtaattat gacggggtac
    20461 atgcacccgg tatacaaacg ctggcgaaag ccttgcgtga gtttgctgac gttcaggtgg
    20521 tcgcccccga tcgtaaccgc agcggcgctt caaattctct gacactggaa tcctccctgc
    20581 gcacgtttac ctttgaaaat ggtgatattg ctgtgcaaat gggaaccccg accgattgtg
    20641 tctatcttgg cgtgaatgct ctgatgcgtc cgcgcccgga cattgttgtg tccggaatta
    20701 acgccgggcc gaatctgggg gatgatgtta tttattccgg tacggtagcc gccgcgatgg
    20761 aaggccgtca tttaggtttt ccggcgctgg ccgtctcgct tgacgggcat aaacattacg
    20821 acactgccgc ggcggtaacc tgttcaattt tgcgcgcact gtgtaaagag ccgctgcgca
    20881 ccggacgtat tcttaatatt aacgttcagg atttaccctt ggatcaaatc aaaggtattc
    20941 gcgtgacgcg ctgcggtaca cgacatccgg cagatcaggt gatcccgcag caagatccgc
    21001 gcggcaatac gctgtactgg attggcccgc cgggcggtaa atgtgatgcc ggtccgggga
    21061 ccgattttgc tgcggtagat gagggctatg tctccatcac gccgctgcat gtggatttaa
    21121 ctgcgcatag cgcgcaagat gtggtttcag actggttaaa cagcgtggga gttggcacgc
    21181 aatggtaagc agacgcgtac aagcacttct ggatcaatta cgtgcgcaag gtattcagga
    21241 tgagcaggtg ctgaatgcac ttgccgccgt gccgcgtgaa aaattcgttg atgaagcgtt
    21301 tgaacaaaaa gcctgggaca atatcgcctt gccgataggt caggggcaga caatttcgca
    21361 gccgtatatg gtggcgcgaa tgaccgaatt actcgagctg acgccgcagt cgcgggtgct
    21421 ggaaattggc actggttcgg gatatcaaac ggcaatcctg gcgcatcttg tccagcatgt
    21481 ttgctcggtt gaacggatta aaggcttgca gtggcaggca cgtcgccgcc tgaaaaatct
    21541 tgatttacat aatgtttcaa cccgtcatgg tgatggatgg caaggttggc aggcacgtgc
    21601 gccgtttgac gctatcattg ttacggcggc accgccggaa attccaactg cgctaatgac
    21661 gcagctggac gaaggcggga ttctcgtctt acccgtaggg gaggagcacc agtatttgaa
    21721 acgggtgcgt cgtcggggag gcgaatttat tatcgatacc gtggaggccg tgcgctttgt
    21781 ccctttagtg aagggtgagc tggcttaaaa cgtgaggaaa tacctggatt tttcctggtt
    21841 attttgccgt aggtcagcgt atcgtgaaca tcttttccag tgttcagtag ggtgccttgc
    21901 acggtaatta tgtcactggt tattaaccaa tttttcctgg gggataaatg agcgcgggaa
    21961 gcccaaaatt caccgttcgc cgcattgcgg ctttgtcact ggtttcgcta tggctggcag
    22021 gctgttctga cacttcaaat ccaccggcac cggtcagctc cgttaatggc aatgcgcctg
    22081 caaatactaa ttctggtatg ttgattacgc cgccgccgaa aatggggacg acgtctacag
    22141 cgcagcaacc gcaaattcag ccggtacagc agccacaaat tcaggctact caacaaccgc
    22201 aaatccagcc agtgcagcca gtagctcagc agccggtaca gatggaaaac ggacgcatcg
    22261 tctataaccg tcagtatggg aacattccga aaggcagtta tagcggcagt acctataccg
    22321 tgaaaaaagg cgacacactt ttctatatcg cctggattac tggcaacgat ttccgtgacc
    22381 ttgctcagcg caacaatatt caggcaccat acgcgctgaa cgttggtcag accttgcagg
    22441 tgggtaatgc ttccggtacg ccaatcactg gcggaaatgc cattacccag gccgacgcag
    22501 cagagcaagg agttgtgatc aagcctgcac aaaattccac cgttgctgtt gcgtcgcaac
    22561 cgacaattac gtattctgag tcttcgggtg aacagagtgc taacaaaatg ttgccgaaca
    22621 acaagccaac tgcgaccacg gtcacagcgc ctgtaacggt accaacagca agcacaaccg
    22681 agccgactgt cagcagtaca tcaaccagta cgcctatctc cacctggcgc tggccgactg
    22741 agggcaaagt gatcgaaacc tttggcgctt ctgagggggg caacaagggg attgatatcg
    22801 caggcagcaa aggacaggca attatcgcga ccgcagatgg ccgcgttgtt tatgctggta
    22861 acgcgctgcg cggctacggt aatctgatta tcatcaaaca taatgatgat tacctgagtg
    22921 cctacgccca taacgacaca atgctggtcc gggaacaaca agaagtgaag gcggggcaaa
    22981 aaatagcaac catgggtagc accggaacca gttcaacacg cttgcatttt gaaattcgtt
    23041 acaaggggaa atccgtaaac ccgctgcgtt atttgccgca gcgataaatc ggcggaacca
    23101 ggcttttgct tgaatgttcc gtcaagggat cacgggtagg agccacctta tgagtcagaa
    23161 tacgctgaaa gttcatgatt taaatgaaga tgcggaattt gatgagaacg gagttgaggt
    23221 ttttgacgaa aaggccttag tagaagagga acccagtgat aacgatttgg ccgaagagga
    23281 actgttatcg cagggagcca cacagcgtgt gttggacgcg actcagcttt accttggtga
    23341 gattggttat tcaccactgt taacggccga agaagaagtt tattttgcgc gtcgcgcact
    23401 gcgtggagat gtcgcctctc gccgccggat gatcgagagt aacttgcgtc tggtggtaaa
    23461 aattgcccgc cgttatggca atcgtggtct ggcgttgctg gaccttatcg aagagggcaa
    23521 cttggggctg atccgcgcgg tagagaagtt tgacccggaa cgtggtttcc gcttctcaac
    23581 atacgcaacc tggtggattc gccagacgat tgaacgggcg attatgaacc aaacccgtac
    23641 tattcgtttg ccgattcaca tcgtaaagga gctgaacgtt tacctgcgaa ccgcacgtga
    23701 gttgtcccat aagctggacc atgaaccaag tgcggaagag atcgcagagc aactggataa
    23761 gccagttgat gacgtcagcc gtatgcttcg tcttaacgag cgcattacct cggtagacac
    23821 cccgctgggt ggtgattccg aaaaagcgtt gctggacatc ctggccgatg aaaaagagaa
    23881 cggtccggaa gataccacgc aagatgacga tatgaagcag agcatcgtca aatggctgtt
    23941 cgagctgaac gccaaacagc gtgaagtact ggcacgtcga ttcggtttgc tggggtatga
    24001 agcggcaaca ctggaagatg taggtcgtga aattggcctc acccgtgaac gtgttcgcca
    24061 gattcaggtt gaaggcctgc gccgtttgcg cgaaatcctg caaacgcagg ggctgaatat
    24121 cgaagcgctg ttccgcaagt aagtaagcat ctgtcagaaa ggccagtctc aagcgaggct
    24181 ggcctttttc ttttgtttgg gaagcttgtg tttttgtcgc ctggataaga cacgtcagcg
    24241 tcctacccgg caatcagcat tctatgtgct catcatcttg cgcaccaact gcataaacag
    24301 ctcctgctct tctccactca aacgccccaa aaattcctca tccacgctgt caccaatagg
    24361 tatcgccgca gcaagtagct tttctccttc ggcagtcagc cagacaaagc gtcgccgctt
    24421 gtcagcgacg tcatgttcac gtctgactaa gccacgattt tccattcttg ccaacatttc
    24481 tgctagcgtt gctttggtgc tgactgctgc ttctatcagt gccacctgtt caataccagg
    24541 tttatcagca atggcgcgca tcaccgcgta ctgtggtttg gtcaggtcag gtaactcgtg
    24601 ctgccagcga gccgtgtgct gctgaaaaag ctgtcgtaac tgatggaacg ctttatttcg
    24661 taacgccatg taaactccgg gctaattctc tgtcgctatc atagcggttt tttagtgacg
    24721 aagaagatgt tgttactttt caatccttgc cgctgtggaa atgaaggagt atgttaataa
    24781 taacgttcgc atacgaataa ttaagagaat aagcaatgaa actgatcgtc gggatgacag
    24841 gggctaccgg tgcgcctctt ggtgtggcat tactgcaagc gctgcgtgag atgccgaatg
    24901 tcgagactca tctggtgatg tcgaagtggg cgaaaaccac cattgaactg gaaacgcctt
    24961 acagcgctcg cgatgttgct gccctcgcag acttcagcca taacccggcg gatcaggcgg
    25021 cgatcatctc atccggttct tttcgtaccg acggcatgat cgttattccg tgcagtatga
    25081 aaacgctcgc cggtatccgc gctggttacg ccgatggcct ggtagggcgc gcggcggacg
    25141 tcgtgctcaa agaaggccgc aaactggtgc tggtgccgcg tgaaatgccg cttagcacca
    25201 tccatctcga aaatatgctc gcactttcac gcatgggcgt ggcgatggtg ccgccgatgc
    25261 ctgcctttta taaccatccc gaaacggtag atgacattgt ccaccatgtg gtagcccgcg
    25321 tgctggatca atttggcctc gaacatcccc acgccaggcg ctggcaagga ttgccgcagg
    25381 cccggaattt ttctcaggag aatgaataat ggcatttgat gatttacgca gctttttaca
    25441 ggcgcttgat gaccacggcc agttactgaa aatcagcgaa gaagtgaacg ctgagccgga
    25501 tctggcagca gcagctaacg ccaccgggcg tatcggcgac ggcgcgcccg cgctgtggtt
    25561 tgataatatt cgcggcttta ccgatgcccg cgtggcgatg aataccatcg gttcctggca
    25621 gaaccacgcg atttcccttg gcctgccacc aaacaccccg gtcaaaaagc agattgatga
    25681 gtttatccgc cgctgggata acttcccgat tgccccggag cgccgcgcca atccagcctg
    25741 ggcgcagaac accgtcgatg gcgaagagat caacctgttc gatattctgc cgctgtttcg
    25801 cctgaatgat ggcgatggcg gtttctatct cgacaaagcg tgcgtggttt cccgcgatcc
    25861 gctcgacccg gataacttcg gcaagcagaa cgtcggcatc taccgcatgg aagtgaaggg
    25921 caagcgtaag ctcggcctgc aaccggtgcc gatgcacgat atcgccctgc atctgcataa
    25981 agcagaagag cgcggtgaag atctgccgat tgcgatcacg ctcggtaacg atccgatcat
    26041 cacgctgatg ggggccacgc cgctgaaata cgatcagtct gagtatgaaa tggcaggcgc
    26101 gctgcgtgaa agcccgtacc cgatcgccac cgctccgctg accggttttg atgtgccgtg
    26161 gggttcagaa gtgatcctcg aaggggttat cgaaagccgt aaacgcgaaa tcgaagggcc
    26221 gttcggtgag tttaccgggc actactccgg tgggcgcaac atgaccgtgg tgcgcatcga
    26281 taaagtctct tatcgcagca aaccgatttt cgaatcactc tatctcggta tgccgtggac
    26341 cgaaatcgac tacctatgac taggtaattt attcaagata accgggaaga gattaaaaaa
    26401 gacgggaata gattggaatc aacaagttag gaactcaagg ttttcaagat aaagcgagtc
    26461 caaaaatgtt aaacggttcg atatcaataa aacattcaag ataactcaca ggttcttaca
    26521 atagccttta aagaccgttc aaaaacgatt caggtgttta ttatagcgct gatgggtgca
    26581 agcgccaaaa ccaatgtaat tttagcacag ctaggcactt gcactgggtt atgcgctggc
    26641 tgctttctga ggtagattta agtgatcgcc cttagcttcg gacaacgcgt aaatctctct
    26701 aaccttttcg tctggcaaat taccaaggct taacgctatg gctctacgaa ctccaactag
    26761 agcgtgcaac tcttggatgt gctcatccag aagaatgagt aattgctccg ctccttttct
    26821 cccaagaaca tcagctactt gttgtttttc ttgtggttta agaaatgaca agaacattag
    26881 aatttgagca tcaacattat ccgcattctc ttttttgggt tctttggaat cttttagttc
    26941 aacatcaccc tctcccgtca tgagccattc caaggtaaca ttttcgcttt ctgctatctt
    27001 ttttaactga tacgggcgtg gtatcggtgc gtaatcccgc ctgttgtaat aattttttaa
    27061 agtgccttca ttgatccccc atgcccgagc agccgcattt cttgaagggt atcgttctac
    27121 taacctaaag attctttcga taatcggttc ttttccatcg gcaggaaaag aaccttgggg
    27181 tgcttttctc attcttggtt cctttctatc taattcattg ataaataata aaattaaaaa
    27241 ataaaccata aaaacaccaa agaacccttt gcatggaatc tttggtatct taatctttat
    27301 ccatacggat atccgaccgg atatccgcgc gggtaatttt gtaggttaaa ggaaagatga
    27361 gacaaagaga agatagtgac cgcgattggc cttcgctgaa gattcagctg gcgctcaaag
    27421 ataacgggtg gtcttccttc cgggagcttt cactccatgc tggttatgct caggattcac
    27481 tgaaagcggt agttcgcaca ccaaacaaaa aatacgaacg cgtaatttct gacgctatag
    27541 gtgtaccgcc agaaatcatc tggcccagcc ggtatcgtaa cgatggattt atcaggaagg
    27601 cgtcgtgata tgcagtcgta cgttaccgta aatgacctgc tcggcgtacc aggaatgcca
    27661 gccaccacaa aaggcatccg acaggcgtta cagcgttttt ctcgtgatct tggtgacgtt
    27721 agtcgccgtc gggaaggcac aaaagccatt gagtaccaca ttgattgtct gcctgaaata
    27781 acacgaaagg cactgcgtga acgctatgtg gaacagctgg tggctacaga gaacaatgtt
    27841 tctgaagtga aagctgttac cagaaaaaca cgcaatcctg acgctgtcca ggcaatcgaa
    27901 gcatatcgcg gttctccaca actgatggaa gaacgcctga atgcgctgac cgaaaaccag
    27961 cgctgggtat ctgaagcaag agctgcgctg gtggtggaag tgctgaagct ggaaagcgcc
    28021 ggtaaccccg ggcgactgaa agccattaac tttcttgttg aaaaagcccg taaaggtgag
    28081 ctgccggagc gcctgcaaca ggccgcagtt aacgccaatg caaaacgtgg cgctaatcgt
    28141 actatcagcc gcgatccact ttaccagtgg gttctgaaat acaaccagtc gcagaacgcc
    28201 gccgaacggc tgctgttact ggcaccggga aaacgcgacg aaataaaacc ggaagaaatc
    28261 agctggctgc ctgaatttct ggcgcagtat cgccaggtga acggcagacc catgtcagaa
    28321 gcctacgagg attttgtcgc agaatggcag cgtcgccatg cggacgaacc gtacatgctg
    28381 gaggtgatgc cttcctatga tgttgtccgg tatgccatga aaaaactgcc ggaagtggtg
    28441 aaacaaaaag ggcgcgttac cggcagtgaa taccgtcagc ttgaaggatt cacccgccgc
    28501 gactggacag ctatgccggt gaattatgtc tggattggtg acggtcacgg tatgaagctg
    28561 aaatgtgcgc atccgatcca cgggcgtcca ttctcaccgg aagtcacgtt tgtgatcgat
    28621 ggtggaacac ggtttgttgt tggctggagt cttgaccttg ccgaaaacgt gttcgccgtt
    28681 gccggtgcga tacagcacgg cattcgcaac cacggaaaac cgttcctgta ttactcggat
    28741 aacggttccg gtgaaacggc tgacatgctg gacaaagagg tcgtgggtat tctgccacgc
    28801 ctggggatta aacaccctac cggtattgcc ggtaatccac agggacgcgg cattattgaa
    28861 cgcctgaacc ggacactgcc gatgcgtata gccaggaggt atcgcaccta ttttggcaag
    28921 ggtgctgacc gggaatcgtt gcgtgtactt aaccgtgatc tgcgttcagc gtttaacgcc
    28981 ctgcaacagg acaagcctct gaacgaccgc cagaaagcgg caatgcgcga gctgccatcg
    29041 tgggcggagc ttattgaggc tattcgtgaa ggcgtggaat ggtacaacaa ccgcccgcat
    29101 tctgagctgc cgatgaaacc gaacggacgg cattacagcc cgacagagtt cagaaaaaaa
    29161 cgccaggcag aagaagacac cgaaattgag tggctgagtg atcttgagct gcgcgacatg
    29221 ttccgcccaa tggtggaacg accggtaaga cgctgcgaaa ttcagtggct gaacaatatt
    29281 tactacgcgc cggagttgcg tgacgaacac ggtcgcaaag tgcttatcag ttatgacatt
    29341 catgacgccg agcgaattac ggtacgtcgc aaggacggca gctttatctg tgaggcgata
    29401 tggaacggca ataaacgcgc agcctttgct gtcagtgctg aatatcacaa acaacagcag
    29461 cgtattaaag gaatgcgcaa acgcgctgag gaaaaaattc gtgatgctga agatgaaggt
    29521 attcagattc tggaacacaa acaggctgaa ccctggctga gtaatgttta tcgcccggtt
    29581 ggtaatgtgg tggcagttca gcaaccggaa tatgaagaag agcatgacga agaatttgaa
    29641 cgcgatttcc ggctggggat gcaaaaactt tttgccatgc aggaagaaga tgatccgctg
    29701 gcctgaataa aaaaacagtc cgcgggaacg gactgttaaa taaaggaaac aaagctgatt
    29761 tgaaataacc gaggaaatac tatgactgat attgatgatg tttgaggtgt actggcaata
    29821 gcggacacta ccatttgttc tttttttaag cagccatctg atgatatttt tccctgaagg
    29881 ctgccgggga gatatttgag gtgtactggc aatagcggac actaccattt gttctttttt
    29941 taagcag
//
LOCUS       DAEBXN010000042        29732 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.042, whole genome shotgun sequence.
ACCESSION   DAEBXN010000042 DAEBXN010000000
VERSION     DAEBXN010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29732
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.042"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            72..353
                     /locus_tag="KJL99_001948"
     CDS             72..353
                     /locus_tag="KJL99_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBD4436688.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPLMEKLKHAAEKIRSQFA"
     gene            319..1396
                     /locus_tag="KJL99_001949"
                     /pseudo
     CDS             319..1396
                     /locus_tag="KJL99_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
     gene            complement(1789..2130)
                     /locus_tag="KJL99_001950"
     CDS             complement(1789..2130)
                     /locus_tag="KJL99_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HBD4436689.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(2143..3015)
                     /gene="copD"
                     /locus_tag="KJL99_001951"
                     /pseudo
     CDS             complement(2143..3015)
                     /gene="copD"
                     /locus_tag="KJL99_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            complement(3019..3393)
                     /gene="yobA"
                     /locus_tag="KJL99_001952"
     CDS             complement(3019..3393)
                     /gene="yobA"
                     /locus_tag="KJL99_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HBD4436690.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            3532..3762
                     /gene="holE"
                     /locus_tag="KJL99_001953"
     CDS             3532..3762
                     /gene="holE"
                     /locus_tag="KJL99_001953"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HBD4436691.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            3864..4520
                     /locus_tag="KJL99_001954"
                     /pseudo
     CDS             3864..4520
                     /locus_tag="KJL99_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
     gene            4544..5206
                     /gene="exoX"
                     /locus_tag="KJL99_001955"
     CDS             4544..5206
                     /gene="exoX"
                     /locus_tag="KJL99_001955"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HBD4436692.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSLLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGLKYSNMALYKTRKLNVQTPLGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERYPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(5203..7263)
                     /gene="ptrB"
                     /locus_tag="KJL99_001956"
     CDS             complement(5203..7263)
                     /gene="ptrB"
                     /locus_tag="KJL99_001956"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HBD4436693.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPKVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDTLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(7473..8132)
                     /locus_tag="KJL99_001957"
     CDS             complement(7473..8132)
                     /locus_tag="KJL99_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HBD4436694.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVSGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDANERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            complement(8459..8815)
                     /locus_tag="KJL99_001958"
     CDS             complement(8459..8815)
                     /locus_tag="KJL99_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HBD4436695.1"
                     /translation="MKKRGAFLGRLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVCVDCKAGMAEYQRR"
     gene            complement(8882..9172)
                     /gene="yebG"
                     /locus_tag="KJL99_001959"
                     /pseudo
     CDS             complement(8882..9172)
                     /gene="yebG"
                     /locus_tag="KJL99_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            9306..10484
                     /gene="purT"
                     /locus_tag="KJL99_001960"
     CDS             9306..10484
                     /gene="purT"
                     /locus_tag="KJL99_001960"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HBD4436696.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSKSLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKSDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRHLGVVLATSESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(10540..11181)
                     /gene="kdgA"
                     /locus_tag="KJL99_001961"
                     /gene_synonym="eda"
     CDS             complement(10540..11181)
                     /gene="kdgA"
                     /locus_tag="KJL99_001961"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HBD4436697.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(11218..13029)
                     /gene="edd"
                     /locus_tag="KJL99_001962"
     CDS             complement(11218..13029)
                     /gene="edd"
                     /locus_tag="KJL99_001962"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HBD4436698.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPENKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(13264..14739)
                     /gene="zwf"
                     /locus_tag="KJL99_001963"
     CDS             complement(13264..14739)
                     /gene="zwf"
                     /locus_tag="KJL99_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HBD4436699.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKVGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            15077..15946
                     /gene="hexR"
                     /locus_tag="KJL99_001964"
                     /gene_synonym="yebK"
     CDS             15077..15946
                     /gene="hexR"
                     /locus_tag="KJL99_001964"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HBD4436700.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            16074..17516
                     /gene="pyk"
                     /locus_tag="KJL99_001965"
     CDS             16074..17516
                     /gene="pyk"
                     /locus_tag="KJL99_001965"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HBD4436701.1"
                     /translation="MSKRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPTDVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVPFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(17647..18618)
                     /gene="lpxM"
                     /locus_tag="KJL99_001966"
                     /gene_synonym="msbB"
     CDS             complement(17647..18618)
                     /gene="lpxM"
                     /locus_tag="KJL99_001966"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707715.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HBD4436702.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(18738..20060)
                     /gene="mepM"
                     /locus_tag="KJL99_001967"
     CDS             complement(18738..20060)
                     /gene="mepM"
                     /locus_tag="KJL99_001967"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HBD4436703.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(20076..21008)
                     /gene="znuA"
                     /locus_tag="KJL99_001968"
     CDS             complement(20076..21008)
                     /gene="znuA"
                     /locus_tag="KJL99_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HBD4436704.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            21087..21842
                     /gene="znuC"
                     /locus_tag="KJL99_001969"
     CDS             21087..21842
                     /gene="znuC"
                     /locus_tag="KJL99_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HBD4436705.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNCPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            21839..22624
                     /gene="znuB"
                     /locus_tag="KJL99_001970"
     CDS             21839..22624
                     /gene="znuB"
                     /locus_tag="KJL99_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HBD4436706.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQQVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(22771..23781)
                     /gene="ruvB"
                     /locus_tag="KJL99_001971"
     CDS             complement(22771..23781)
                     /gene="ruvB"
                     /locus_tag="KJL99_001971"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HBD4436707.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGAQEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(23790..24401)
                     /gene="ruvA"
                     /locus_tag="KJL99_001972"
     CDS             complement(23790..24401)
                     /gene="ruvA"
                     /locus_tag="KJL99_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HBD4436708.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDTSSETLIREALRAAL"
     gene            complement(24540..24605)
                     /gene="yobI"
                     /locus_tag="KJL99_001973"
     CDS             complement(24540..24605)
                     /gene="yobI"
                     /locus_tag="KJL99_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HBD4436709.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            24675..25277
                     /locus_tag="KJL99_001974"
     CDS             24675..25277
                     /locus_tag="KJL99_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HBD4436710.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(25279..25800)
                     /gene="ruvC"
                     /locus_tag="KJL99_001975"
     CDS             complement(25279..25800)
                     /gene="ruvC"
                     /locus_tag="KJL99_001975"
                     /EC_number="3.1.22.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HBD4436711.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGMGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLTRGRLR"
     gene            complement(25835..26575)
                     /locus_tag="KJL99_001976"
     CDS             complement(25835..26575)
                     /locus_tag="KJL99_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBD4436712.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(26604..27056)
                     /gene="nudB"
                     /locus_tag="KJL99_001977"
     CDS             complement(26604..27056)
                     /gene="nudB"
                     /locus_tag="KJL99_001977"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HBD4436713.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(27174..28946)
                     /gene="aspS"
                     /locus_tag="KJL99_001978"
     CDS             complement(27174..28946)
                     /gene="aspS"
                     /locus_tag="KJL99_001978"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HBD4436714.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADGMGALLLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            29256..29510
                     /locus_tag="KJL99_001979"
     CDS             29256..29510
                     /locus_tag="KJL99_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HBD4436715.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPVTLFVPLIMGC"
BASE COUNT         7279 a         7644 c         7310 g         7499 t
ORIGIN      
        1 ttattaaaaa tcaaccacac ccgccagtat tctgtatatt tactggcggt catatcgtga
       61 gaggtatggc aatgaatctt gtgacactca aaacgtgggg aaaactcaga tatccggata
      121 acccaccatc aatatcaacg ctgagacgat gggcaaggaa tggaaacatt tatcctgcac
      181 ctgaactaca cgggaggagt tacagggtgg ttccggaggc tttctatatc aacccaaata
      241 aggttgatac cgatataaca caccatcagc ctaatgggcg acaagggaga gacagtccgt
      301 taatggagaa gttaaaacat gcagcggaaa aaatacgatc ccaatttgcc tagaaactta
      361 acatatcgaa ggagagacaa agcatattac tggcgcaacc ctctgacgaa agaagaattt
      421 acactaggta aaatttcaag aagagatgca gtagcgcagg caattgaagc aaatcattat
      481 atatacaaaa actactctcc tgctgcccta attgaaaagc ttaaaggggt cgactcattt
      541 actatggcag gctggattga acgttacaaa acgattctta taaggagaaa agcgtccaga
      601 aatacttata aaactcgggt aaatcaactg gagacaataa aagaaaaatt aggaggggtt
      661 ttactgacag aaataaccac tcgccatatc gccgagtttc ttgatttgta gattgaagga
      721 gggaaaaaca cgatggcagg atcaatgcgt tctgtgttat ctgatatgtt ccgcgaagcc
      781 attgttgaag gacgtatatc tcaaaatcca gtaacgccaa caagagcacc gaaaatagta
      841 gttacaagag aacggctgaa actaaagaca tacaactgca tcagggaggc agcagatcaa
      901 cttccggcat ggttcccatt agctatggac ttagcccttg taacaggaca acgtcgcgaa
      961 gacataacga atatgcggtt cagtgatatt tatgatgatc gtctccacat caggcaaatt
     1021 aagacaggaa tgatgattgc tatccccctg tcactcagcc ttcctgtcgc tggtttacga
     1081 cttggtgcag tagttgaaca gtgccgcatg gtaagtaggg gggattatct aatcagtgcc
     1141 gggattagaa aaaacagccc tgacggcagc attcacccgg acggcctgac aaagaaattt
     1201 gtcgcagcca gaaaattaac aggtatcact gttcagtgaa aacccaccaa cttttcacga
     1261 aatcagaagc ctggctggac gattgtacaa agaaatatgt ggagaagaat ttgctcagcg
     1321 tctacttggc cacacatcgg agaagacaac aaaaatgtat cttgatgaga gagaaaaaac
     1381 gtacttactg ctctgatttt aacgtaaatg gattgttaaa tgtattttgg ttgtgatata
     1441 accaaaaaag accggaatac agaaattcga gtaaatttcg gggaatttcg gggagacgtt
     1501 tgcaactaat tgattttaaa tacaattaaa aaaagaccga atacgattcc tgtattcggt
     1561 ccagggaaat ggctcttggg agagagccgt gcgctaaaag ttggcattaa tgcaggctta
     1621 gttgccttgc cctttaagaa tagatgacga cgccaggttt tccagtttgc gtgcaaaatg
     1681 gtcaataaaa agcgcggtgc tcatcagctt aaatgttaaa aaccgcccgt tctggtgaaa
     1741 gaactgaggc ggttttttta ttggaaatca aaaggctatt ttaggtaatt aacagagttt
     1801 ttcagctcgt tctataaacg gtgccagact cattttttcg ccgggattgt taggatcatc
     1861 aatctgaatc accgaaatgg gttgggcatt ggtcttccca ctggcaactt ccttttgtgc
     1921 gatatcgttt aaaggatact gcacgagggt acttgggttg atgacataca aagcattacc
     1981 cggtcggcaa gtcagcatta cctcttcgcg attaaacgcc catttgtctt tacccacttc
     2041 aaaacgactc acggttatca cctgtggagc cgccagcgcc gctgcagaac tggtgagtaa
     2101 cagaaacgcc agaatacttt ttttcatcat tttcgtaatc catcaaaaag gttcgagagt
     2161 cgcaagcagg ctgatgatca ccagcactac cgcacctatc gcccattcga gtttagtcat
     2221 ccacacaaac cagggtgccg cacgatcttc atcctgcctc atgcgcggta caagaacata
     2281 acgattagcc agcgcaatga ccaccataat catcaccagg atggctttga gcaataagag
     2341 ttggccccag taagtcgtaa gcgtgggggg aaatccggta atcaacaatg cattaagcac
     2401 gccgcttgcc agtacgccga tcaccgcaaa atgcccgcac caggaaaaac gcatcagcgc
     2461 ctgaatagcc tagtgtcgcc aacggccttt gatgagttgc atacaccaga gcaccggcag
     2521 taatccccca aaccacgccg ccgcacaaat caggtgaatc gcgtgattag tctgatggat
     2581 tttcgctgtt accccttcat tcagcgtcgc ataccccact cccgccagca gaataaattg
     2641 cgcggtagtg agcataaaca gcaaacgtgg catattccgc ggttgcataa gggcgacgat
     2701 caacgttacg agggcgagaa caatttgcca taaccagacg ccaccaaact gcgtttgcaa
     2761 aacagcctgc caaatgtttg gcgaaaatac atctgtccat cccgttccca tcaacccgcc
     2821 ctgaacggcg agcattgcag tggcactgat caaactccag acggcagcgt gctgttgtaa
     2881 acgcagagaa cgcttcgtca gcaagcgacg aatcattaag ggggccagcc aggcaccata
     2941 cattgcaaag ccaaagacca gcatcaacga ggtgaaatgg ataaatcgca gcgcgatcca
     3001 ggtaaacgcc agcatgattt atttcacgct aaaggtgtag tgtcctttcg ttttatgccc
     3061 gtccaccgaa acaacatgcc agtcaacggt gtaggttccg ggtttcagcg aatcggccag
     3121 tggaacaatc agttgctttt ggtcctgctc attccgcttc gctggtaatg ttttaatatt
     3181 ttcgtttttt ggccccgtga tttttgcacc actgaatcct gtttcaacgc cttccgagaa
     3241 gtttaaagtg attgcctgcg gggcagctgt cacttgcgcg tttgccgcag gatactgatg
     3301 cgttaaatgc gcatgtgccc agacggaagg tgtcaccaac gaagtggtca ggattgccag
     3361 cgcgtagcga agggagcgtg cagttgaagc catattatct attccttttt gtaataactt
     3421 ttttacagag cataaccttg tctaatgtct gagtcgagga tcatcaattc cggcttgcca
     3481 tccaggctca ctcttagtaa cttttgcccg cgaatgatga ggagattaag aatgctgaag
     3541 aatctggcta aactggatca aacagaaatg gataaagtga atgtcgattt ggcggcggcc
     3601 ggggtggctt ttaaagaacg ctacaatatg ccggtgatcg ctgaagcggt tgaacgtgaa
     3661 cagccagaac atttgcgcag ctggtttcgc gagcggctta ttgcccaccg tttggcttcg
     3721 gtcaatctgt cacgtttacc ttacgagccc aaacttaaat aaaacttata cagagttaca
     3781 ctttcttaca taacgcctgc taaattatga gtgttttcta aaccgccccc ataatttgca
     3841 gtcattttga aaaggaagtc attatgtcgt tctggaaagt tgcagcagcg caatatgagc
     3901 ctcgtaaaac ctcactcacc gaacaggtag ctcatcatct ggagtttgtg agggccgccg
     3961 ccaggcaaca atgtcagctg ttagtttttc catcgctttc tttactgggg tgtgattatt
     4021 cgcgacgtgc ccttcctgcc ccacccgatc tttcactgtt agacccgctt tgttatgccg
     4081 caacgaccta gcgaatgacc atcattgccg gccttcccgt tgaatataac gatcgcttta
     4141 ttcgtggaat tgcggtgttc gcaccgtggc gaaaaacgcc tgggaactac catcagagtc
     4201 atggtgcatg tctgggccgt cgttccagaa ccatcacggt ggtcgatgaa caaccgcaag
     4261 gtatggatat ggacccgacc tgttcactgt ttacgacagg gcaatgtctg ggagaacccg
     4321 acctgctggc gtcggcccgt cgcttacaat ttttttcaca tcagtactcg attgccgtgc
     4381 tgatggcaaa tgcccgtggt aacagtgcac tatgggatga atatggtcgt ctcatcgttc
     4441 gcgccgatcg tggttcatta ttgttagtcg gtcagcgttc atcacagggt tggcaaggcg
     4501 atatcattcc attacgctag gctttttcgg cctggatcat gccatgttgc gcattatcga
     4561 tacagaaacc tgcggtttgc agggagggat cgttgagatt gcctctgttg atgtcattga
     4621 cggaaaaatc gtcaacccca tgagcctcct ggtgcgcccc gatcgtccta ttagtccaca
     4681 agcgatggcg attcatcgca tcaccgaagc catggtcgcc gataaaccgt ggattgaaga
     4741 tgtgatccca cactattacg gtagtgaatg gtatgtcgcg cacaacgcca gctttgaccg
     4801 ccgcgtactg cctgagatgc ccggtgagtg gatttgcact atgaaactgg cccgtcgttt
     4861 gtggccaggg ctcaagtaca gcaatatggc gttatataaa acacgcaagc tcaatgtaca
     4921 gacaccgctg ggcctgcatc atcaccgcgc gttgtatgac tgttatatca ccgccgcgtt
     4981 gcttatcgat attatgaaca cctccggctg gacggcagaa cagatggccg atatcaccgg
     5041 acgtccgtcg ttgatgacaa ccttcacctt tggcaaatac cgtggcaaag cggtttccga
     5101 cgttgccgaa cgctatccgg gctatctgcg ctggttattt aataacctgg acagcatgag
     5161 cccggagctg cgtttaacac tgaaacatta tctggaaaat acttaatccg caggcgtagc
     5221 gggtaatgtt ccctgcgcca gcgcgaccag aaaagcatat tccatcgcta cgccttcgta
     5281 cgatttaaag cggccagatt taccgccatg gcctgagtcc atgtcggtac agagcaataa
     5341 aagatggtca tcggttttca gctcgcgcaa tttagcgacc cattttgccg gttcccaata
     5401 ttgcacctga gaatcgtgta aaccggtcgt taccagtaaa tgcggataag cctgtgcggt
     5461 gacgttgtca tacgggctgt agcttttcat gtactcgtaa tattgcggat cctgcggatt
     5521 tccccactct tcaaactcac cagtggtaag aggaattgat tcatcaagca tcgttgttac
     5581 aacatcaaca aacggtacct gggcgataac gccgtggaat aattccgggc gttgattaat
     5641 tgcaacgccc atcaacatgc cccccgcact tccgcccatc gcataacaga gcgaaggaga
     5701 gccatagccc agttttaaca atgcatcgca ggcatcaaga taatcattaa acgtattttt
     5761 cttcttcaga aatttaccgt cttcgtacca ttgttgcccc agctcaccac cgccgcgaac
     5821 atggacaatg gcgtagacaa agccacgatc taacaaactc aagcggctaa aactgaaatc
     5881 ggcatcaata cttgcgccgt aagaaccata gccatacacc agcaacgggt tgtgtccttt
     5941 gcgaaaatgt ttgcgatgat agaccagcga aaccggaact tcgacgccat cacgggcgac
     6001 tatccacagg tgttcactgc ggtaattcgc cgcatcaaaa ccaggcactt ctgtttgttt
     6061 taatacacga cgctcaccgg tatccatatc cagttcaaac aaagtgtctg gtgtggtcat
     6121 ggaagaataa ccataacgca atcgcgcggt ttcaggttct ggattgtagg caatccaggt
     6181 cacataggcc ggatcatcaa aggcaatgcc aatgacttcc cgggtcttgc ggttaatttg
     6241 gcgcaaactg gttaacccgc gctgacgctc ttcaaccacc agccagtcgg taaacagcgt
     6301 aaacccttcc agcatgatgt tttcgcgtgg cggaattaac tcttcccact gttgctcatc
     6361 acgcatacgg gtacggtata agccaaagtt tttgccgtgg cgattggaac gcagataaaa
     6421 acgatgctgg tagtgatcaa ggctgtattc gtgatctttg cggcgcggca gaaaaacaaa
     6481 cggctcggca tcggccattt ccgcgtccag caggcgaact tcactggtgg tggcgctggc
     6541 cagatgaatg acgacatagt gcttcgacgt cgttttatgc aggctgacat aataggtatc
     6601 gtctttttct tcgtagatca gtgtatcttg cgatgctggc gtaccgatgg cgtgacgcca
     6661 gacctgataa ggcagcagcg tcaccggatg cttgcgaaca tagtagaaag tccaggagtc
     6721 atttgcccag acaaagctgg gttcaacgtt atccagcagt tccgggtacc agttacctgt
     6781 ttccagatta cgaaaacgaa tgccgtactg gcgtcgggaa agaaaatctt ctgccagcgc
     6841 cataatggtg ttatcgggcg taatcgccat tccgcccatc gaataaaact cactatgagc
     6901 tgcgcgcttg ttggcatcga gcaatgtttc ccactcatcc cactcttcgc tgaatgccga
     6961 ttgacgctgg tagatagcat attcacagcc tggttcataa atatgccgat agcggtagcc
     7021 attttttatg tagggcgcag aaacttctct ttgcggaatg cggtcgatga tttcctttaa
     7081 gatgcgatcc tgcaaggctt gttgtgaggc catcacccga tgaccgtaac tattttcttg
     7141 ttgcaggtag tccaggactt ttggctgaga acgcgtatcg tcccgcagcc agtagtaatt
     7201 atcgatgcgc gtatcgccat gaagcgtcat ggcgtgggga atgcgggcgg cttttggtag
     7261 catgttattg ttctttctgg ttggaaacat cttataaggg tggcaaaact caccggggat
     7321 gcaagcgaaa cagggtcgtc attgcttaga tgatgacagg taatggcgcg gatatcgaat
     7381 gttatgcaga cagagaaaat cagcctgttc aaatggctgt gcggttctgg atagcccgaa
     7441 atagtcaact tcaggctatc cagagagcgg aattattccg ccaaagtgcg tttttgctgt
     7501 tcgagatcgc gttcaatgcc ttcacgaaca tcctggggga ttttcagcgc gtcacccagt
     7561 gcattcaggt aactgcgttc cataaaatgg tcaatatcaa tagccgcgca gctcaggaaa
     7621 tagatttcca gcgcctcttc ttcattgcgg accccggtag ccaggcgttg tggatccagc
     7681 ggttgttcga ttgcctgctc aatgagtaca cgcccctgct cttccacgcc ggcttcacgc
     7741 aattgctggt cgattgccgc acgttcattg gcatcaatat gaccatcact tttagcggca
     7801 aagaccagcg caaggatcaa acgttccgta cgctcatcca gcggcgtact ttgcgcgcca
     7861 aactgcggtt cgtcctgatg cgccgcgcga attttatctt tgtatttatt ccacagcacc
     7921 gtacccgcta ccgctccgcc gccaaccagt aacgcgttgg tgccatattt cgtaagaagt
     7981 ttacgtgctg atttatttgc gaccagcagc ccagccagtc cgcctaatgc gcctgacact
     8041 aacagtttgc ccaatccctg atccgcagac gaggaggtag aagaactgct ttgcccaagc
     8101 agggattgca gttgatttaa ccagttagcc atatttgctc ctcaataacc atttttagtg
     8161 cctgtagcat agcggatgag gatgtcagaa aatgtatatc gagacaaaag atgcgcaaat
     8221 aagcggggcg gtaggccgtt attcaaagaa attcgcgcca ggtaaggtat ttactgtata
     8281 tcaggtgata agggtatttt aggtgaaagt agtttgtcgt tttgagtggc attgtttgat
     8341 gctgattgcc cgatgcgacg ctgttgcgtc ttctcaggcc tacgattgtg gtgcgttctg
     8401 ggacggacaa ggcactcgcg ccgcatccgc caacggtcaa tcgaattatc agaagccatt
     8461 aacgccgctg atattccgcc attcccgctt tgcagtccac gcagacctgg taatgaatat
     8521 cggcactttt accacgcacg gttagcggta ctgaccattt atcatcttta ccctgaatgt
     8581 cctgcaaact gacccaggcc acgggatcgg cctgaccgac aattttttga tcatctgccc
     8641 agcgcgccac gcgattttgt tgataatcac gttttacgct cgcggcaatt ccggcagcat
     8701 ccagaccttc acactttggg aaagtgaccg acttgctggt ttcattattg gcagcgaaaa
     8761 ctgatgcgca ggcagaaacc aacaaccgcc ctaaaaacgc ccctcttttt ttcatgtttt
     8821 tctccatagc acaatgattc aggagaaagc atggtacaaa ttgtcaggag cgcaagttgc
     8881 ttcaggccgc gtgtgaggca tcttcctctt cggattcagc accgacgacc tgtggggacg
     8941 gtaatttgcc ggttttcaga atggtgctca gcacctcttt ttgttctgcc agccatagcg
     9001 aaagggcttc acgttgctcg tcttccattt gcactggaga atttgtcagc taggtgtcaa
     9061 gcagatccgc cgtatcaagc attttgtcat aagcatcggc ttcctttttg ctggtaaacg
     9121 acattttctc ttcgccctca cgaatgacta cgtatttaac ttcaaccgcc atttgcagcc
     9181 tctcataata actgaaattt tatacagtat atttcttttc ggttgagaaa tcaacatcag
     9241 caataaagac acacgcaaac gttttcgttt atactgcgcg cggaattaat caggggatat
     9301 tcgttatgac gttattaggc actgcgctgc gtccggcagc aactcgcgtg atgttattag
     9361 gctccggtga actgggtaaa gaagtggcaa tcgagtgtca gcgtctcggc gtagaggtga
     9421 ttgccgtcga tcgctatgcc gacgcaccag ccatgcatgt cgcgcatcgc tcccatgtca
     9481 ttaatatgct tgatggtgat gcattacgcc gtgtggttga actggaaaaa ccacattata
     9541 tcgtgccgga gatcgaagct attgccaccg atatgctgat ccaacttgaa gaggaaggac
     9601 tgaatgttgt cccctgcgct cgcgcaacga aattaacgat gaatcgcgag ggtatccgtc
     9661 gcctggcggc agaagagctg cagctgccca cttccactta tcgttttgcc gatagcaaaa
     9721 gccttttccg cgaggcggtt gccgccattg gctatccctg cattgtaaaa ccggtgatga
     9781 gctcttccgg caaggggcag acgtttattc gctctgcaga gcaacttgct caggcatggg
     9841 aatacgctca gcaaggcggt cgcgccggag cgggccgcgt aattgttgaa ggcgtcgtta
     9901 agtctgactt cgaaattacc ctgctaaccg tcagcgcggt ggatggcgtc catttctgtg
     9961 caccagtagg tcatcgccag gaagatggcg actaccgtga atcctggcaa ccacagcaaa
    10021 tgagcccgct tgcccttgaa cgtgcgcagg agattgcccg taaagtggtg ctggcactgg
    10081 gcggttatgg tctgtttggt gtcgagctgt ttgtctgtgg tgatgaggtg attttcagtg
    10141 aggtctcccc tcgtccacat gataccggga tggtgacgtt aatttctcaa gatctctcag
    10201 agtttgccct gcatgtacgt gccttcctcg gacttcctgt tggcgggatc cgtcagtatg
    10261 gtcctgcagc ttctgccgtt attctgccac aactgaccag tcagaatgtc acgtttgata
    10321 atgtgcagaa tgccgtaggc gcagatttgc agattcgttt atttggtaag ccggaaattg
    10381 atggcagccg tcatctgggg gtggtactgg ctacttcaga gagtgttgtt gacgccattg
    10441 aacgcgcgaa gcacgccgcc ggacaggtaa aagtacaggg ttaaacccgg caaaaaaacg
    10501 ctacaaaaat gcccgatcct agatcgggca ttttgacttt tacagcttag cgccttctac
    10561 agcttcacgc gccagcttag taatgcggtc gtaatcgccc gcttccagcg catctgccgg
    10621 aaccagccag gaaccaccga tgcacagcac gcttttcagt gccaggtagt cacggtagtt
    10681 agccggagaa ataccacccg tcgggcagaa acggacctgg gagaacggac ccgcgatcgc
    10741 ctgcagggct ttcacgccgc cgttagcttc agccgggaag aatttgaact ctttcaaacc
    10801 gtagtccata cccagcatca gttcggaaac agtgctgatc cccggaatca gaggaatagt
    10861 cccttcggta gcagctttca gcagcggctc ggtcagaccc gggctaattg cgaactgtgc
    10921 acccgcttca gtgacttctg ccagctgctg tggattcagc accgtaccgg cacccacaat
    10981 cgcttcaggc acttctttgg cgatagcacg gatagcgtca actgcacact cggtacgcag
    11041 agtcacttcc agaacgcgca ccccaccagc aaccaacgct tttgccatcg gcaccgcgtg
    11101 ttccagtttt tttaccacga taaccggtac aaccgggccg gtggtcagga ttgattctgc
    11161 acttgttttc cagtttttca tcagagtttt ctctcgcctg attacaaatt tgtcgtctta
    11221 aaaagtgata caggttgcgc cctgttcggc accggacagt ttttcacgca aggcgctgaa
    11281 taattcacgt cctgttccca cgcgtgacgc gctcaggtca ggaatgtgcg gttcgcgagc
    11341 agccagttcc gcttcgtcta ccagcagtgt cagttcgcct gtctgtccat tcacacgaat
    11401 gatgtccccg tcgcgcactt ttgccagcag cccgccatcg taggcttctg gtgttacgtg
    11461 gatagctgac ggcactttac ctgaagcgcc ggagagtcgt ccatcggtaa ctaacgcaat
    11521 tttgaaacac cggtccaata atacaccaag tggcggcatg agtttatgta attctggcat
    11581 tccgttcgct tttggcccct gatgacggac aacaacgaca caatcgcggt ccagcaaacc
    11641 cgcttcaaag gccggcataa cgtcatgctg gctttcaaaa acaaccgctg gcgcttcaat
    11701 cacctggttc tcaaccggca cggcagaggt tttcataacc gcacggccca ggttaccgct
    11761 taacactttt gtcccaccat gatgagagaa aggttgttcg aaggaagcga tcacattgct
    11821 gtcgagtgat ttttccgccc cttcccgcca gtccagttca ccattattca gccatggttc
    11881 aagggtataa cgagacagac caaagcctgc caccgtattg acatcttcat gcagcaggcc
    11941 tgctttgagc agttcacgca ccagaaccgg tacgccacct gccgcctgga agtggttaat
    12001 atcggccgga ccgttcgggt agagacgtgc catcagcggt acaacatcag aaaggtcaga
    12061 gaagtcatcc cagttaatct gaataccggc cgcgcgcgcc atcgccacca ggtgcatggt
    12121 gtggttagtg gaaccaccgg tcgccagcag tgcaacgata ccgttcacca ccactttctc
    12181 atcgatcatc ttaccgatcg gcatccattc attaccatta ccggtcatgc gtgtaacctg
    12241 acgcgcagcg gcagcggtca aagcatcgcg cagcggagaa tccggatgaa caaaagaaga
    12301 gcctggcaac tgcatcccca taaattccac caccatctgg ttggtgttgg cagtaccgta
    12361 gaaagtacat gttcccggcg catggtaaga cgcggcttct gactccagta aggccatgcg
    12421 gtccacttta ccttcggcat aaagctggcg aatacgcact ttttctttat ttggcaaacc
    12481 gcttgccatc ggtccagacg gcacaaacac cgcaggcaaa tgaccaaacg acagggctgc
    12541 catcgtcaga cccgggacaa tcttgtcgca cacaccgagg aacagagcac catcaaacat
    12601 gttatgggac agccccaccg ccgcagacat cgctatcact tcgcggctta gcagcgacaa
    12661 ttccattcca tcctgcccct gggtgacacc atcacacatc gccggaacac cgcccgcaac
    12721 ctgaccaacc gcattcgctt catgcagggc tttacgaatg atttctggat agtgttcata
    12781 aggctggtgc gcggagagca tgtcgttata ggaggtgatg atggcgatat tgttacgcaa
    12841 catgcttttc aaagaggctt tgttttctgg ctggcaggca gcgaaaccgt gtgccaggtt
    12901 accgcatgcc aactgcgaac gatgaacggt cgacgttttc gcttgttcta tccgggcgag
    12961 ataagcagag cgagtctcgc gcgaacgttc aatgattcga tttgttacgc gtaacaattg
    13021 tggattcata aaggctcctg aaattgagtt gtcagagcag gatgattcac aacgcgtttc
    13081 attcagagga tttatgagtg aaacgcctgt aaccggagct catagggcaa aaacgtttca
    13141 gtcagtgtaa taaaaaaagc ctcgtgggtg aatccgcacg aggcctgaaa gtgtaaaaat
    13201 tgttctacaa tctgtgcaag atcatgttac cggtaaaata accataaagg ataagcgctg
    13261 atattactca aactcattcc aggaacgacc atcacgggta atcatcgcca ccgaggcaac
    13321 gggtccccag gttccggcct gatacggttt cggcgcatca ttgtccatcg cccacgcctc
    13381 agtaatggag tctacccatt tccaggcttc ttccacttcg tcgcgacgta caaacagtgc
    13441 ctgaatacca cgcatggttt ccagcagcaa acgttcatag gcatccgcca gatgcgtctg
    13501 attaaaggtt tctgaatagc tcagatccag cttggtgatt tgcaggttat gtttgtggtc
    13561 aaggccagga actttattca gtacctggat atccacgcct tcatcaggtt gcagacggat
    13621 agtcagttta ttctgcggca gatcctgcca cgactcttta aacagattca gttcaggtgt
    13681 tttgaaatag accacgactt cagaacattt ggtcggcaga cgtttaccag tacgcaggta
    13741 gaatggcaca ccggcccagc gccagttatc aatgtcgacg cggatcgcca cgaaggtttc
    13801 tgtattgctg cttttgttcg cgccctcttc ttccagatat cccggcactt ttttgccctg
    13861 ggcgaagccc gcagtatatt gcccgcgtac ggttttttcg cgtacgttgg agcggtcgat
    13921 gcggcgcagg gacttcagta ctttcacttt ttcatcgcgg atgctgtctg cgctcaggtc
    13981 agatggcgga gacatcgcaa tcatgcagag aatttgtagc aggtggttct gaatcatgtc
    14041 acgcatctga ccggctttat caaaatagcc ccagcgccct tcgatcccca cttcttctgc
    14101 cacggtaatc tcaacatgat caatggtgcg attgtcccag ttattcacaa acagggagtt
    14161 agcaaaacgc agcgccaaca ggttcagcac cgtttcttta ccaagatagt ggtcgatacg
    14221 gtaaacctgg cactcctcga agtattcgcc aacctgatca ttgatttcct gcgaggtcgc
    14281 cagcgacgtc cccagcggtt tctccatgac tacgcgtgcc ggtttagcat tcagttttgc
    14341 ctcgccaagc cctttgcaaa ttgcgccaaa agtgctgggc ggcatggcaa agtagttaat
    14401 agtgatacga tttttttgat ccagcatcgc gccgagacgg ttgaatgcag cagtgtcatt
    14461 gacatcgaga ttacaaaaat ccagacgtgc actcagggtg tcccataaac cttcatcaat
    14521 ggtttctttc atgaaagttt cgagcgcctc gcggacaact ttggtatatg ccgctttatc
    14581 ccagtcagca cgccctacgc cgataatccg ggtgtccggg ttgagctgac cgactttttc
    14641 cagttgatac agggaaggca gcaatttacg acgcgcaagg tcgcctttcg cgccgaaaat
    14701 gaccaggtca caggcctggg ctgtttgcgt taccgccatg tcattctcct taagttaact
    14761 aacccggtac ttaagccagg gtatacttgt aattttctta cggtgcactg tactgctttt
    14821 acgagcttgc gaaaactgta aacgcttatc cacccgtgcg attacgggaa aagcgcgcaa
    14881 agcgcggcaa aactgataaa aaaatcatcg ttttccattc ggtaaaaatc tgacactgat
    14941 catgttatga aaaaaaataa caactttttt atctgctttt gtcattaaca cggcacacag
    15001 gcgtaatatc tgacaaaact gcatttcgat ttctttcagt gcggaaatcg tcattacccg
    15061 tgagtctctt tacatcatga atatgctgga aaaaatccag tctcagctgg aacatttgag
    15121 caaatcagag cgcaaagttg ccgaggtcat tctggcttcg cccgataacg cgatccattc
    15181 gagtattgct gctctggcgc tggaagccaa tgttagcgaa ccgacggtga atcgtttctg
    15241 tcgcagcatg gacacgcgcg gttttcctga ttttaaactt catctggcac aaagtctggc
    15301 gaatggcact ccctatgtta atcgcaacgt caatgaagat gacagcgttg aatcatacac
    15361 ggggaaaata tttgagtccg caatggcaac gctcgatcat gtccgtcatt cactggataa
    15421 atctgccatc aaccgcgccg tcgacttgct cactcaggca aaaaaaatcg cctttttcgg
    15481 attaggctct tctgccgccg ttgcccacga tgcgatgaat aagttctttc gttttaatgt
    15541 tccggtggtg tactccgatg atatcgtgct gcaacgcatg agttgtatga attgtagcga
    15601 cggagacgtg gtggtgctga tttctcacac aggaagaaca aaaaatctgg tcgagctggc
    15661 gcagctggca cgcgaaaacg acgccatggt gattgccctc acctctgcgg gtaccccgct
    15721 cgcccgggaa gcaacgctgg caattaccct cgacgtaccg gaagatactg acatttatat
    15781 gcccatggtt tctcgacttg cacagctgac cgtgatagat gtgctggcga caggatttac
    15841 tttgcgacgc ggtgcaaaat tcagagataa cttgaagcgg gtcaaggaag cgctgaagga
    15901 atcgcgtttt gataagcagt tacttaattt aagtgacgat cgctaaaagc gactgtcact
    15961 gtcctaatct tatacgacat ccgaatgaga ttaatttatc gccatcgcgg cgttatttca
    16021 ttcggatttc atgttcaagc aacacctggt tgtttcagtc aacggagtat tacatgtcca
    16081 aaaggcttcg cagaacaaaa atcgttacca cgttaggccc agcaacagat cgcgataata
    16141 atcttgaaaa agttatcgcg gcgggtgcca acgttgtacg tatgaacttt tctcacggct
    16201 cgcctgaaga tcacaaaatg cgcgcggata aagttcgtga gattgccgca aaactggggc
    16261 gtcatgtggc tattctgggt gacctccagg ggcccaaaat ccgtgtatcc acctttaaag
    16321 aaggcaaagt tttcctcaat attggggata aattcctgct cgacgccaac ctgggtaaag
    16381 gtgaaggcga caaagaaaaa gtcggtatcg actacaaagg cctgcctact gacgtcgtgc
    16441 ctggtgacat cctgctgctg gacgatggtc gcgtccagtt aaaagtactg gaagttcagg
    16501 gcatgaaagt gttcaccgaa gtcaccgtcg gtggtcccct ctccaacaat aaaggtatca
    16561 acaaacttgg cggcggtttg tcggctgaag cgctgaccga aaaagacaaa gcagacatta
    16621 agactgcggc gttgattggc gtagattacc tggctgtctc cttcccacgc tgtggcgaag
    16681 atctgaacta tgcccgtcgc ctggcacgcg atgcaggatg tgatgcgaaa attgttgcca
    16741 aggttgaacg tgcggaagcc gtttgcagcc aggatgcaat ggatgacatc atcctcgcct
    16801 ctgacgtggt aatggttgca cgtggcgacc tcggtgtgga aattggcgac ccggaactgg
    16861 tcggcattca gaaagcgttg atccgtcgtg cgcgtcagct aaaccgagcg gtaatcacgg
    16921 cgacccagat gatggagtca atgattacta acccgatgcc gacgcgtgca gaagtcatgg
    16981 acgtagcaaa cgccgttctg gatggtactg acgctgtgat gctgtctgca gaaactgccg
    17041 ctgggcagta tccgtcagaa actgttgcag ccatggcgcg cgtttgcctg ggtgcggaaa
    17101 aaatcccgag catcaacgtt tctaaacacc gtctggacgt tcagttcgac aatgtggaag
    17161 aagctattgc catgtcggca atgtacgcag ctaaccacct gaagggcgtt acggcgatca
    17221 ttaccatgac cgaatcgggt cgtaccgcgc tgatgacctc ccgtatcagc tctggtctac
    17281 caattttcgc catgtcgcgc catgaacgta cgctgaacct gactgctctc tatcgtggcg
    17341 ttacgccggt gccctttgat agcgctaatg acggcgtagc agctgccagc gaagcggtta
    17401 atctgctgcg cgataaaggt tacttgatgt ctggtgacct ggtgattgtc acccaaggcg
    17461 acgtgatgag taccgtgggt tctactaata ccacgcgtat tttaacggta gagtaagtac
    17521 gttgccggat gcggcgaaaa cgccacatcc ggcctatagt tcaatgatag ttcaacagat
    17581 ttcgaatatt ctgaagcaaa cttgaactta tcatcaggcg aaggcctctc ctcgcgagag
    17641 gcttttttat ttgatgggat aaagatcttt gcgcttatac ggctggattt cgcccggttt
    17701 gcgagttttc agcaatttta gtatccaggt gtattgttct ggtcgcggac caacaaaaat
    17761 ctcgacttct tcattcatcc gccgctcaat cgtatgatca tccgcctcta acagatcatc
    17821 catcggtggg cgcacctgaa tcgtcagacg atgcgtcttg ccatcataaa tcggaaacag
    17881 cggtacaacg cgcgcacggc atactttcat caaacgacca atcgcgggca acgtcgcttt
    17941 ataggtggca aagaaatcca caaattcgct gtgctctggg ccatgatcct gatcgggtaa
    18001 ataatatccc cagtacccct gacgtaccga ctggatgaat ggtttaatac cgtcatttct
    18061 cgcatgcaga cgaccgccaa agcgacgacg caccgtgttc cagacataat caaaaaccgg
    18121 gttgccctga ttatggaaca tcgctgccat tttctgccct tgcgaggcca tcagcatggc
    18181 aggaatatcg acggcccaac cgtgcggcac cagaaagata actttctcgt tattacgccg
    18241 catctcttcg atgatctcca gcccttgcca gccaacgcgc ggctgaattt tctccggccc
    18301 gcgtattgcc aactcagcca tcattgccat cgcttgcggc gcggtggcaa acatctcatc
    18361 aacaatcgct tcgcgttcag cttcactacg ttctggaaag cagagcgaca gattgattaa
    18421 cgcacgacgg cgtgagcttt ttcccagtcg tccggcaaaa cgtcccagcc gtgccagaat
    18481 gggatcacgg aactttggcg gcgttaaagc gatacccgcc atcgctgcta cacccagcca
    18541 tgctccccag tagcgcgggt ggcgaaagga tttatcaaac tcaggaatgt attcgctatt
    18601 attttttttc gtttccatgc ttttccagtt tcggataagg caaaaatcaa tctggtgata
    18661 gtgtagcggc acaacttgcc ccgcaccaaa taaaaaaagc cggtactgac tgcgtaccgg
    18721 ctgcgaatgg atgttaatta atcaaaccgt agctgcggca caatctcttt ggcctgcgcc
    18781 aggaattcgc gacgatcgga gccggtcagc ccttcggtac gcggcagttt tgccgtcagc
    18841 gggtttacgg cctgctggtt tatccatact tcatagtgca gatgcggccc agttgaacgt
    18901 ccggtattac cggaaagcgc gatacggtcg ccacgtttca ccttctgtcc cggtttcacc
    18961 agaatcttgc gcaagtgcat ataacgcgtg gtgtagctgc gaccatgacg aatagccaca
    19021 taataacctg ctgcgccact gcgtttggca accaccactt caccgtcacc cactgaaagc
    19081 actggcgtac cttgtggcat ggcgaaatca acacctctgt gtggtgcaac gcgacctgtc
    19141 accggattag ttcgacgcgg gttaaagtta gacgagatac ggaactgttt cgccgtcggg
    19201 aatcgcaaga atcctttcgc cagaccagta ccgttacgat cgtagaattt gccatcctca
    19261 gcgcggattg cgtaataatc tttaccttct gaacgcaaac gtacgcccag cagctggctt
    19321 tgctcacgtt taccatcaag catttctcga gacattaata ccgcaaattc atcgcctttt
    19381 ttcagtttgc ggaaatccat ttgccactgc atggctttaa tcactgcgct cacttcggcg
    19441 ctggttaaac cggcgtttct ggcgctggca acaaagctcc ctccgacggt acctttcagc
    19501 agattgttaa cccactctcc ttgctgcatt tcgctggtca ttttaaaacc gttagcggca
    19561 gtacggtcat aggttcgggt ttcacgacga gacacttccc aggtgaggcg ctgcagttcg
    19621 ccgtccgcgg ttaatgtcca ggagagttgt tgaccgattt tcaggttacg caattctttg
    19681 tcggcagcag caagttgggt gatatcaccc atatcaatac catactgatt gagaatgctg
    19741 cttagcgtat cgccagtgga aacaacatat tcatgcacgc ccgcttcacc ggcgattttg
    19801 tcatccagtt cgtcctgggg aatggcttca tcttcttgtg cagcttgatc aatcggctca
    19861 ctggcttcag gtaagagcga acgaatttcg ttctgttcca gctcaatggt tttgacaatt
    19921 ggcgtggcat cacggtgata aacatagggc cgccagacag cgacggccag agtaagaacg
    19981 gtgagcgacc ccaacataac gcggtgtggt cgcggtaaat tattaaacgc cagggcgaca
    20041 gagcgggcta tctgttgcac gtaatcactt cctcattaat ctcctttcag gcagctcgca
    20101 tactggttgg ctaattgact caggaattct gaatagcttg ttttacccag tttgatattc
    20161 gtccccaggg gatccaacgt tcccatacga acggatgtcc ctcgtgcgac gctctcaacg
    20221 accgctggcc tgaactgtgg ctcagcaaaa acgcaggttg ctttttgctc aaccaactgt
    20281 gttcttattt catgtaaacg ctgcgcgcca ggttgaatct cagggttaac ggtaaaatga
    20341 ccaagcggtg tcagtccgaa ctgtttttcg aaatagccgt aagcatcgtg aaaaacgaaa
    20401 taacctttcc ccttgagcgg cgcgagctcg ttaccaacct gcgtttcggt tgaggccaat
    20461 tgtgcctcaa aatccttcag gttggcgtca agtttggctc gactttgcgg cataagttcc
    20521 actaattttc catggattgc aaccgctgta gcccgcgcta tctctgggga aagccaaaga
    20581 tgcatgttga aatcgccgtg atggtgatct tcgtcacttt tttccgcgtg gtcgtgatca
    20641 tcatcatcgc cgtgaatact tttcatcagt agcggtttca catcttcaag ctgcgcaatc
    20701 gttacctgct tcgcttctgg taatttgctt accggttttt gcataaacgc ttccatctcc
    20761 gggccaaccc aaacgactaa gtccgcgttc tgtaagcgtt ttacatccga tggacgcagt
    20821 gaataatcat gttctgaagc gccgtcaggc agtaaaacct ccgtttctgt tacaccatca
    20881 gcaatggcag aagcgatgaa cccaacgggt ttaagcgaag cgacaacggc ggcatctgcg
    20941 gcctgtgttg cacctcccca gagagcggcg gataatgctg cgaaaagaag cgttttttta
    21001 tgtaacataa tgcgaccaat catcgtaatg aatatgagaa gtgtgatatt ataacatttc
    21061 atgactactg caagactaaa attaacatga caagtctggt ttccctggaa aatgtctcgg
    21121 tttcttttgg ccaacgccgc gtcctctctg atgtgtcgct ggaacttaaa cctggaaaaa
    21181 ttttgacttt acttgggcca aatggcgcag gtaagtcgac actggtacgg gtagtgctcg
    21241 ggctggtaac acccgatgaa ggggttatca agcgcaacgg aaaactgcgc atcggctatg
    21301 taccgcagaa gctgtatctc gacaccacgt tgccactgac cgtaaaccgt tttttacgct
    21361 tacgccctgg tacacataaa gaagatattt tgcctgcact gaaacgtgtc caggccgggc
    21421 acctgattaa cgcaccgatg caaaagctct ctggtggcga aacgcagcgt gtactattag
    21481 cgcgagcatt gttaaattgc ccgcaattat tagtgctgga tgaacccact caaggcgtgg
    21541 atgtaaatgg ccaagtggcg ttatatgacc ttattgacca gttgcgtcgt gaactggatt
    21601 gtggcgtttt aatggtttct cacgatctgc atctggtgat ggcaaaaacc gatgaagtgc
    21661 tgtgcctgaa tcaccacatt tgttgttccg gcacaccgga agttgtttcc ctgcatccgg
    21721 agtttatttc aatgtttggt cctcgtggtg ctgaacaact gggtatctat cgccatcatc
    21781 ataatcatcg tcacgattta cagggacgaa ttgttttgcg tcggggaaat gatcgctcat
    21841 gattgaatta ttatttcccg gttggttagc cgggatcatg ctcgcctgtg ccgcgggtcc
    21901 gctgggttcg tttgtagtct ggcgtcgtat gtcttatttc ggtgatacgc tggctcatgc
    21961 ctcattactt ggtgtcgcgt ttggtttgtt gctggacgtg aatccattct atgcggtgat
    22021 tgccgttacg ctgctgctgg cgggcggtct ggtatggctg gagaagcgtc cacagctggc
    22081 gatcgacacg ttattaggga ttatggcgca cagtgccctg tcgctgggcc tagtggtcgt
    22141 tagtctgatg tctaacattc gtgttgattt gatggcttac ctgttcggtg atttactggc
    22201 agtgacgcca gaagatctca tctctattgc gattggcgtg gtcatcgtgg tggctatttt
    22261 gttctggcaa tggcgcaatt tgctgtcgat gacgattagc ccggatctgg cgtttgttga
    22321 tggtgtgaaa ttacagcaag tgaaattgtt gttgatgctg gtgacggcat tgacgattgg
    22381 tgtagcgatg aaatttgtcg gtgcgttgat tattacttcg ttgctgatta ttcctgcagc
    22441 tactgcgcgt cgctttgccc gtacgccgga acagatggct ggtgtcgctg ttttggtggg
    22501 gatggtggca gtaactggcg gtttaacctt ttccgcgttt tacgatacgc cggcgggtcc
    22561 gtcggtggtt ctatgtgcgg cactgttatt tattatcagt atgatgaaaa agcaggccag
    22621 ctaatctatc gctgaacaca tttgtcggat gcggcgcgag cgccttatcc gacctacggt
    22681 tcgctatctc tggctggcct gataagacgc gaacagtgtc gcatcagaca cactgccagt
    22741 gtcggatgcg gcgcgagcga ccaatccgac ttacggcatt tctggcggcg ttatgccaaa
    22801 gtgattccac gcccgcgtcg tcgccatacg cccgcgcggt gtacgctgca aaaagccttg
    22861 ctgaatcaaa taaggttcca gtacatcttc aatggtttca cgttcttcgc caatggctgc
    22921 cgccaggtta tccagaccta ccggcccacc aaagaactta tcgattaccg ccagcaacaa
    22981 tttgcggtcc atataatcga aaccttcagc atcgacattc aacatatcca gcgcctgagc
    23041 agcgatatct gccgagatgg tgccatcgtg cttcacttcg gcgaaatcac gcactcgacg
    23101 cagcagacgg ttggcaatac gtggcgtacc gcgagcgcga cgagctactt cctgcgcacc
    23161 gtcatcactc atctcaagcc ccataaagcg tgcactgcga ctgacgatat attgcagatc
    23221 cggcacctga taaaactcca gacgttgcac aataccaaaa cggtcgcgca acggtgatgt
    23281 cagcgaacct gcgcgcgtgg ttgcgccaat cagggtaaac ggcggcaaat caattttaat
    23341 ggagcgtgcc gccggacctt caccaatcat gatatccagt tggtagtctt ccattgccgg
    23401 gtacagcact tcttcaacaa ctggcgataa acggtggatc tcatcaataa acagcacgtc
    23461 atgcggttca aggttagtga gcatcgcagc caaatcgccc gccttttcca gcaccggacc
    23521 agaagtcgtg cgtaaattaa cgcccatttc attggcgaca atgttggcaa gcgtagtttt
    23581 acccaacccc ggaggaccaa aaatcaacaa atgatcgagg gcatcgccgc gcagtttcgc
    23641 tgctttgatg aaaatctcca tctgtgaacg aacctgcggc tgaccaacat actcttccag
    23701 taatttaggg cgaatggcgc gatctgctac atcttccggc aaagtggtac cggcagaaat
    23761 cagacggtct gcttcaatca tcctttacct cataacgcgg cgcgtagggc ttcgcgaatt
    23821 aatgtttcac tgctggtgtc agggcgagcg attttgctca ccatgcggct tgcttcttgt
    23881 ggtttatagc ctagcgccac cagcgcggca actgcttctt gttcagcatc gtcggtcgcc
    23941 gggctagcag gagacgtgag taccaggtcg gcggctggcg taaagagatc gccatgcaaa
    24001 cctttaaatc ggtctttcat ttcaacaatc aagcgttcgg cggttttttt gccaataccc
    24061 ggcagtttca ccagtgcccc cacttcttca cgctcaacgg cattaacgaa ctgctgcgct
    24121 gacattccgg agaggatcgc cagggccaac ttcgggccga cgccgttggt tttgatcaac
    24181 tctttgaaca acgtgcgttc ttgtttattg ttaaaaccgt acagcagttg cgcgtcttca
    24241 cgcaccacaa agtgggtgaa aacgatcgct tcctgacccg cttcagggag ttcataaaaa
    24301 caggtcatcg gcatatgcac ttcatagcct acgccgccca cttcaattaa caccagcggg
    24361 ggttgttttt caatgatgat gcctctgagt ctgcctatca catgacgctc ctgcgtaatg
    24421 aatcaaagat aatgctgtat gataaaaaaa tgctggatag atatccagcg aaggatgaag
    24481 aaaacttgcg aggtgtcccg atgatctgaa aaatggcaca gtataattta ttcaacaggt
    24541 tatattggaa gcaaatattt gaatattaca tattcagtga agaaatgcgt aataaaaata
    24601 tacattgcga ctcctgaaaa ataatttttt tatgctatta cgtatatttg tatctatttc
    24661 aatggaatga caacgtgaat attaattacc ctgctgaata tgaaattgga gatatcgtct
    24721 ttacatgtat aggtgctgcc ttatttggtc aaatatcagc tgcatcaaat tgctggagta
    24781 atcacgtcgg gatcattatc ggtcataacg gtgaagactt cctggttgca gaaagccggg
    24841 ttcccctttc aaccatcacc acgctatctc gttttattaa acgttcgtct aatcaacgct
    24901 atgctataaa gcgattagac gctgggctga cagaacaaca aaaacaacga attgttgaac
    24961 aggttccctc ccggctacgc aaactttacc acacgggttt taaatacgaa tcttcgcgcc
    25021 agttctgttc aaaatttgtc tttgatattt ataaagaggc gctatgtatt ccggtgggtg
    25081 aaatagagac gtttggacaa ctgttaaata gcaatccgaa tgcaaaactc actttctgga
    25141 aattctggtt cttaggttct attccgtggg agcgtaaaac cgtcacgcca gccagtttgt
    25201 ggcatcatcc gggtttggtg ttgattcacg cggagggagt tgaaacgcct cagccggaac
    25261 tgaccgaggc ggtataactt aacgcagtcg ccctctcgtc aggttcagcc gcgattcgct
    25321 catctgcatc gcattctgac taacgtggca gtgggtgatg gcaatcgcca gcgcatcggc
    25381 ggcatccgcc tgtggattag cgggcagttt cagcaaggtg cggaccatat gctgcacctg
    25441 gcttttttcg gcactaccca tacctaccac cgtttgcttt acctgacgtg ccgcgtattc
    25501 aaataccggc aattcctgat tcaccgccgc aacaatcgcc acgccgcgcg cctggcccag
    25561 tttcagggct gagtcagcgt tcttcgccat aaagacttgt tcaatggcga aataatcagg
    25621 ctggaactgg gtgatgattt ccgtcacgcc cgcatagatg agcttcagac gagacggtaa
    25681 atcatccact ttggtgcgta tgcatccgct acccaggtag gacagttgcc tacctacctg
    25741 gcggatgacg ccgtagccgg tcacgcgcga acccgggtca atgccgagaa taatagccat
    25801 cacgcgtctc cgttttgctg tttagcaggc atcatcagag agtcgctgca acctcatcag
    25861 agatttcacc gttatggtaa acttcctgca cgtcgtcgca atcttccagc atatcgatca
    25921 gacgcatcag tttcggcgcg gtttctgcat ccatatcagc tttggtggac gggatcatgg
    25981 aaacttccgc gctgtctgct ttcagacctg ccgcttccag agcgtcgcgc actttaccca
    26041 tttcttccca tgcagtgtag acatcaatcg cgccgtcatc atatgtcaca acgtcttcag
    26101 cacctgcttc cagagctgct tccatgatgg tgtcttcgtc gcctttctcg aaggagatca
    26161 cgcctttttt gctgaacaga taagctacgg aaccgtcagt gccgaggtta ccgccacatt
    26221 tgctgaatgc gtgacgcact tcagcaacgg tacggttgcg gttgtcagac agacattcaa
    26281 tcatgattgc cgtgccgcca ggaccgtaac cttcgtagat gatggtttcc atgtttgcat
    26341 catcatcacc gcccacacca cgtgcaattg cgcggttcag tgtgtcacgg gtcatgttgt
    26401 tagacagtgc tttatccacc gccgcacgca gacgcgggtt agcgtccgga tcgccaccgc
    26461 ccagcttagc cgcggttacc agctcacgaa tgattttagt gaagatttta ccgcgcttag
    26521 catcctgcgc agctttacga tgtctggtgt tggcccattt actatgacct gccataaaaa
    26581 tatctccaga tagccctgcc tgttcaggca gcgttaatta caaactgttc aatcgcctgc
    26641 cggttgctcc aggacttagt gagcgccgcc gcagcaggcg catcaagcca cttgtaagcc
    26701 agatgttcag tgaaaacgat ctgccgctcg tgcggaagcg caagacagaa ccatgattcc
    26761 gtattacgcg tcacgcccgg cgcatagcga tgacgtaaat gtgaaaaaat ttcaaactct
    26821 accgtgcgct gacagtcaat taaggtcagt tgttcagcga caacatcaat ggtaacctct
    26881 tcctttactt cgcgcatggc agcttgcggc gcggtttcac cctcttccac gctgccggtt
    26941 accgactgcc agaaatcggg atcgtcacgc cgctgcaaca tcagcacccg tttcgtatct
    27001 tgtgcataga tgaccactaa gatcgaaacg ggacgcttat acactttatc cttcacgctg
    27061 cctcttcgtt gactgccttc gctcatccca ttcacatagt tatctatgct catgggagtt
    27121 cactcagttg ccgccttgat gcaacgcgaa tgatttcgtg tatttgagtc atatcagtta
    27181 ttctcagcct tcttcacaac ctgaatgctc agctcagcca gtgcagtcgg gttagcaaag
    27241 ctcggtgctt cagtcatcag acacgctgcc gccgtggttt tcgggaaggc gataacgtca
    27301 cggatattgt cggtgccagt cagcagcatg gtcagacggt caagaccgaa tgccagacct
    27361 gcgtgcggcg gagtaccgta tttcagggcg tcgagcagga agccgaattt ctcgcgctgt
    27421 tcctcttcgt tgatacccag aataccaaac accgtctgct gcatatcacc attatggata
    27481 cgtactgaac caccgcccac ttcgtaacca ttgatgacca tatcgtaagc gttcgccacc
    27541 gcattttccg gtgcagcttt cagttctgca gcagtcatat ctttcggtga ggtgaacgga
    27601 tggtgcattg ccgtcaggcc gccttcaccg tcgtcttcaa acatcgggaa gtcgataacc
    27661 cacagcggtg cccatttgct ttcgtcggtc agaccaaggt ctttacccac tttcaggagc
    27721 agtgcgccca tcccgtcggc aacaattttc ttgttgtcgg caccgaagaa aatcatatcg
    27781 ccatcttgcg cggcagtacg atccaggatg gcttcgatga tttctgcatt aaggaacttc
    27841 gctaccgggc tgttgatacc ttccagacct ttcgcgcgtt cgttaacttt gatgtaagcc
    27901 agacctttcg cgccgtagat tttaacgaag ttaccgtatt cgtcgatctg cttacgggtc
    27961 agcgatgcgc cacccggaac acgtagagcg gcaacacggc ctttcggatc gttcgctggc
    28021 cccgcaaata ctgcaaactc aacagatttc agcagatcgg caacgtcggt cagttccatc
    28081 gggttacgca gatccggttt atcagaacca taacggcgtt ctgcttccgc aaaggtcatt
    28141 accgggaaat cgcccagatc cacgcccttc acttccagcc acaaatgacg caccagcgct
    28201 tccatcactt cacgcacttg cggcgcggtc atgaaagaag tttccacatc gatctgagta
    28261 aattcaggct gacgatcagc gcgcaggtct tcgtcacgga agcatttaac gatctgatag
    28321 taacggtcaa aaccggacat catcagcagc tgtttgaaca actgtgggga ttgcggcagt
    28381 gcgtagaatt tacctttgtg cacacgagaa ggcaccaggt agtcacgcgc gccttccggc
    28441 gtggctttgg tcagcatcgg agtttcgatg tcgaggaagc cgtggtcatc cataaaacgg
    28501 cgcaccaggc tggtgatttt agcgcgggtt ttcaggcgtt gcgccatttc cgggcgacgc
    28561 aggtcgaggt agcggtattt cagacgcgct tcttcggtgt tgacgtggtt agagtcaagc
    28621 ggcagaacat ctgcgcggtt gatgatagtc agcgaggacg ccagcacttc aatttcgccg
    28681 gtcgccatat cgcggttaat atttttttcg tcacgcgcac gtacggtgcc cgtaacctga
    28741 atgcagaact cattacgcag ttcagaggcc agctttaacg cgtccgcacg atccggatcg
    28801 aaaaatacct gcacgatacc ttcgcggtcg cgcatatcga tgaagatcag gctaccaaga
    28861 tcacgacgac ggttgaccca accacacaga gtcacctgct gccccacgtg ggacaaacgg
    28921 agctgtccac aatattctgt acgcatgaga tatcccttaa cttagctgcc ggcggatgcc
    28981 ccctgctgcg caggtgacca agtcgcagcg ttagctgtat gtcacaactg aatgaaaaaa
    29041 ggcggctatt atactggaaa ttctgccgca ccgtaagagc ctggcccgcg ctggaacgcc
    29101 tcgttaccac tttatatcgg gcctgaaacc agactctacg ccagtttgct ataaaggtgt
    29161 tgcccgaact cataaaaatt aacaaaattt gtcgttccgc catcggctaa tcgcattaag
    29221 gtgtgaggca cgattttgtt ttttcaggag tcagcatgct tgaacttaat gctaaaacca
    29281 ctgcgttggt ggtgatcgat ttacaagaag gcatcttacc ttttgccggt ggtccacata
    29341 ctgccgatga ggtagttaat cgcgccggga agctggcggc gaaatttcgc gccagcggtc
    29401 agcccgtgtt tctggtgcga gttggctggt ctgccgatta cgccgaagca ttaaaacagc
    29461 cggttgatgc ccccgttact ttattcgtac cccttataat ggggtgttag ccagccagac
    29521 ccggcatgat tactgccccc agtcgtccat gatccggggg gtgatgtcac cgggtctggt
    29581 ggggcgctgg taaccgctaa taggggtcag gtcaggcact tttgccggga ccgtctgtaa
    29641 cgtggatgcc ggtacctgct ccccgtggtt atctggttaa cccatataca agggagacag
    29701 aatgaccgaa tccagcgatt acgaatccgt cc
//
LOCUS       DAEBXN010000043        29366 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.043, whole genome shotgun sequence.
ACCESSION   DAEBXN010000043 DAEBXN010000000
VERSION     DAEBXN010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29366
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.043"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            75..1352
                     /gene="waaA"
                     /locus_tag="KJL99_001980"
     CDS             75..1352
                     /gene="waaA"
                     /locus_tag="KJL99_001980"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HBD4436716.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDAVYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            1360..1839
                     /gene="coaD"
                     /locus_tag="KJL99_001981"
     CDS             1360..1839
                     /gene="coaD"
                     /locus_tag="KJL99_001981"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HBD4436717.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="KJL99_001982"
     CDS             complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="KJL99_001982"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HBD4436718.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="KJL99_001983"
     CDS             complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="KJL99_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBD4436719.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="KJL99_001984"
     CDS             complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="KJL99_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HBD4436720.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(3426..4094)
                     /gene="radC"
                     /locus_tag="KJL99_001985"
     CDS             complement(3426..4094)
                     /gene="radC"
                     /locus_tag="KJL99_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HBD4436721.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            4266..5486
                     /gene="coaBC"
                     /locus_tag="KJL99_001986"
     CDS             4266..5486
                     /gene="coaBC"
                     /locus_tag="KJL99_001986"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HBD4436722.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            5464..5922
                     /gene="dut"
                     /locus_tag="KJL99_001987"
     CDS             5464..5922
                     /gene="dut"
                     /locus_tag="KJL99_001987"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HBD4436723.1"
                     /translation="MMKKIDVKILDPRVRKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            6029..6625
                     /gene="slmA"
                     /locus_tag="KJL99_001988"
     CDS             6029..6625
                     /gene="slmA"
                     /locus_tag="KJL99_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HBD4436724.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="KJL99_001989"
     CDS             complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="KJL99_001989"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HBD4436725.1"
                     /translation="MKSYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(7369..8085)
                     /gene="rph"
                     /locus_tag="KJL99_001990"
     CDS             complement(7369..8085)
                     /gene="rph"
                     /locus_tag="KJL99_001990"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HBD4436726.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            8212..9075
                     /locus_tag="KJL99_001991"
     CDS             8212..9075
                     /locus_tag="KJL99_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HBD4436727.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMKSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            9296..10120
                     /gene="dinD"
                     /locus_tag="KJL99_001992"
     CDS             9296..10120
                     /gene="dinD"
                     /locus_tag="KJL99_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HBD4436728.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFTIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDSVNSKQQANTTHFDVGSKVRQTIQELGGTIPEELPTPQVSIKQLENSVKITEKK"
     gene            10411..11028
                     /locus_tag="KJL99_001993"
     CDS             10411..11028
                     /locus_tag="KJL99_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HBD4436729.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(11025..12707)
                     /gene="ligB"
                     /locus_tag="KJL99_001994"
     CDS             complement(11025..12707)
                     /gene="ligB"
                     /locus_tag="KJL99_001994"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HBD4436730.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVLQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNPLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARNQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            12965..13588
                     /gene="gmk"
                     /locus_tag="KJL99_001995"
     CDS             12965..13588
                     /gene="gmk"
                     /locus_tag="KJL99_001995"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HBD4436731.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            13643..13918
                     /gene="rpoZ"
                     /locus_tag="KJL99_001996"
     CDS             13643..13918
                     /gene="rpoZ"
                     /locus_tag="KJL99_001996"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBD4436732.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQELEAAELQAVTAIAEGRR"
     gene            13937..16045
                     /gene="spoT"
                     /locus_tag="KJL99_001997"
     CDS             13937..16045
                     /gene="spoT"
                     /locus_tag="KJL99_001997"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HBD4436733.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMGVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            16052..16741
                     /gene="trmH"
                     /locus_tag="KJL99_001998"
     CDS             16052..16741
                     /gene="trmH"
                     /locus_tag="KJL99_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HBD4436734.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            16747..18828
                     /gene="recG"
                     /locus_tag="KJL99_001999"
     CDS             16747..18828
                     /gene="recG"
                     /locus_tag="KJL99_001999"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HBD4436735.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(18862..20067)
                     /gene="gltS"
                     /locus_tag="KJL99_002000"
     CDS             complement(18862..20067)
                     /gene="gltS"
                     /locus_tag="KJL99_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HBD4436736.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            20347..21738
                     /gene="xanP"
                     /locus_tag="KJL99_002001"
     CDS             20347..21738
                     /gene="xanP"
                     /locus_tag="KJL99_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HBD4436737.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            21859..23568
                     /locus_tag="KJL99_002002"
     CDS             21859..23568
                     /locus_tag="KJL99_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HBD4436738.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPAPLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVR"
     gene            complement(23621..25939)
                     /gene="yicI"
                     /locus_tag="KJL99_002003"
     CDS             complement(23621..25939)
                     /gene="yicI"
                     /locus_tag="KJL99_002003"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HBD4436739.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPPWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPIDVQWFDGSDSQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(25949..27332)
                     /locus_tag="KJL99_002004"
                     /pseudo
     CDS             complement(25949..27332)
                     /locus_tag="KJL99_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
     gene            27625..27719
                     /locus_tag="KJL99_002005"
     tRNA            27625..27719
                     /locus_tag="KJL99_002005"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27659..27661,aa:Sec,seq:tca)
     gene            27991..29202
                     /locus_tag="KJL99_002006"
     CDS             27991..29202
                     /locus_tag="KJL99_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBD4436740.1"
                     /translation="MNSSKAGGCHGINDIKVKTAKPKDKPYKLADGGGMYLLINTNGS
                     KYWRMKYRFAGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQ
                     MSLKNTFEAIAREWHQLKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMKLLEA
                     LRKMEKRGALEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTA
                     NELPHFLNDLAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVM
                     KMKRPHIVPLSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKG
                     RLTGHGFRHTMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYA
                     DYIDSLSSRES"
BASE COUNT         7271 a         7850 c         7355 g         6890 t
ORIGIN      
        1 aacgggaaaa gtaatggtaa agccacagct aaatacatag aatccccagc acatccataa
       61 gtcagctatt tactatgctc gaattgcttt acaccgccct tctctacctt attcagccgc
      121 tgatctggat acggctctgg gtgcgcggac gtaaggctcc ggcctatcga aaacgctggg
      181 gtgaacgtta cggtttttac cgccatccgc taaaaccagg cggcattatg ctgcactccg
      241 tctccgtcgg tgaaactctg gcggcgatcc cgttggtgcg cgcgctgcgt catcgttatc
      301 ctgatttacc gattaccgtt acaaccatga cgccaaccgg ttcggagcgc gtacaatcgg
      361 ctttcgggaa ggatgttcag cacgtttatc tgccgtatga cctgcccgat gcgctcaacc
      421 gtttcctgaa taaagtcgac cccaaactgg tgttgattat ggaaaccgaa ctatggccta
      481 acctgattgc ggcgctacat aaacgtaaaa ttccgctggt gatcgctaac gcgcgactct
      541 ctgcccgctc ggccgcaggt tatgccaaac tgggtaaatt cgtccgccgc ttgctgcgtc
      601 gtattacgct gattgctgcg caaaatgaag aagatggtgc acgttttgtg gcgctgggcg
      661 caaaaaataa ccaggtaacc gttaccggta gcctgaaatt cgatatttct gtaactccgc
      721 agttggctgc taaagccgtg acgctacgcc gccaatgggc accacaccgc ccggtatgga
      781 ttgccaccag cactcacgaa ggtgaagaga gcgtggtcat tgccgcacat caggcattgt
      841 tacagcaatt cccgaattta ttgctcatcc tggtaccccg tcatccagaa cgcttcccgg
      901 atgcgattaa ccttgtccgc caggctggac taagctatat cacacgctct tcaggggaag
      961 tcccctccac cagcacgcag gttgtggttg gcgatacgat gggcgagttg atgttactgt
     1021 acggcattgc cgatctcgcc tttgttggcg gttcactggt tgaacgtggt gggcataatc
     1081 cgctggaagc tgccgcacac gctattccgg tattgatggg gccgcatact tttaacttta
     1141 aagacatttg cgcgcggctg gagcaggcaa gcgggcttat taccgttacc gatgccacta
     1201 cgcttgcaaa agaggtttcc tctttactca ccgacgccgt ttaccgtagt ttctatggcc
     1261 gtcatgccgt tgaagtactg tatcaaaacc agggcgcgct acagcgtctg cttcaactgc
     1321 tggaacctta cctgccaccg aaaacgcatt gaggttgtta tgcaaaaacg ggcgatttat
     1381 ccgggtactt tcgatcccat taccaatggt catatcgata tcgtgacgcg cgccacgcag
     1441 atgttcgatc acgttattct ggcgattgcc gccagcccca gtaaaaaacc gatgtttacc
     1501 ctggaagagc gtgtggcact ggcacagcag gcaaccgcgc atctggggaa cgtggaagtg
     1561 gtcgggttta gtgatttgat ggcgaacttc gcccgtaatc aacacgctac ggtgctgatt
     1621 cgtggcctgc gtgcggtggc ggattttgaa tatgaaatgc agctggcgca tatgaatcgc
     1681 cacttaatgc cggaactgga aagtgtgttt ctgatgccgt cgaaagagtg gtcgtttatc
     1741 tcttcatcgt tggtgaaaga ggtggcgcgc catcagggcg atgtcaccca tttcctgccg
     1801 gagaatgtcc atcaggcgct gatggcaaaa ttagcgtagc gtttatgccg gatggtatgc
     1861 catccggctc gcatgaatta cttctggcac tgccgacaat aaaacgttgc ccgctgcgca
     1921 tgtttagtcg ccacaatcgg cgtaccgcac acccgacacg gctcaccttt tcgcccgtaa
     1981 acctgcaatt cctgcgcgaa atagcccggt ttaccatcac tttgcagaaa atctttcagc
     2041 gttgtaccac cctgctcaat cgaacgcagc aacaccgctt taatcacccg agctaacaat
     2101 tcacactctg ccagcgacag tgatgacgcc agccgatccg gatggatccc cgccgcaaac
     2161 agtgattcgc tggcatagat attccctacc cctaccacca gcttgttatc catcagccac
     2221 ggtttaatcg ccgttttttt cttcgcgcac ttctgatgca gatactcacc attgaaatcg
     2281 tcgctaagcg gctccggtcc aagatgggcc agcacattat gcccttccag ctctttggtc
     2341 cacagccagg caccaaagcg gcgcggatcg gtgtagcgca gcactttacc gttgctcatc
     2401 accaaatcca catggtcatg cttttcaggg ggaagttctt ctggaaggat gcgcaggctg
     2461 ccagacatcc ccaaatgaat gataatccag ccctcaggca gctccagcag cagatattta
     2521 gcccgccgct gcacgctaag cactggttga tcgcttaatc ggtagatctc ttctgaaacc
     2581 ggccagcgca agcgtccgtt gcgcaccacc gcatgaagaa tggttgcacc aacgagatgc
     2641 ggttctatgc cgcgacggct ggtttcaact tcgggtaatt caggcatagc atctccagga
     2701 atgaacagat gcaatcaata tgggggcaag cagataacaa aaaaccccgc cggagcgagg
     2761 tttttcgtta catcaaagcg agaattattt gattttcgct tctttgtaga tcacgtgctg
     2821 acgaacaact ggatcgaatt ttttcagttc cagtttttcc ggcttagtac gtttgttctt
     2881 cgtagtggta tagaagtgac cagtaccagc agaagaaacc agcttgattt tctcacgaat
     2941 acctttagcc atgatttatt tcctctaagt acttagtact tttcgccacg ggcacgcagt
     3001 tcagccagaa ctgtatcgat gcctttttta tcgattacac gcataccttt agcagatacg
     3061 cgcagggtga caaaacgctt ctcgctctca acccagaaac ggtgagagtg caggttcggc
     3121 aggaaacggc gtttagtcgc gttcagtgcg tgggaacggt tgttaccggt caccggacgc
     3181 ttgccagtaa cttggcagac tcgggacatg tctattctcc aaaaatcaaa ttagctcgag
     3241 cttcgtatgg ggtattggcg cctcgtcagg ctttacagcc cggtcatcgc agttctatgt
     3301 gaactctcga ttgccaggcc caaatgccaa acccgagatt ctcaaaggtg gcgtagtata
     3361 cgctgactca gcgatgtgct caagtcccga acagacaaag atcccgaagg atcgcgcata
     3421 gcgggttaaa tccagccgcg ttcggcaaaa gaaacatact ctccacgccc aatcacgata
     3481 tggtcgagca cgcgtaaatc catgaactga caactcttta ttatccgttc agtaatgagt
     3541 ttatccgctt tactgggttc agcacaaccc gaagggtgat tatgtgcaag gatcagcgcc
     3601 gaggcgttta tttttatcgc ttcgcggata atttcccgag gatggacttc aacatggttt
     3661 agcgtgccgg aaaaaagacg gctatgcgtt ataacccggt gttgtgagtc gagaaagatc
     3721 accataaaga tctcccgctc ctcacccgtg agctggcttt gtaaaaattc ccgcgtcatc
     3781 tccgggctga gtaaagggct ttcttcacgc atccgcacgt tgtagtaacg tcgcgccagt
     3841 tcagcaatcc cctttaactg ggcaaatttc gctacgccaa ttccatgaac accactaaat
     3901 tgttcatatt cagaggttaa caagccataa agagagccga aattctccag catctctttt
     3961 gccagggtta atacatcttt accgcgcgtt ccggtacgca gaaatagcgc cagcagctcg
     4021 acatccgtta aggcgctaat accaaacttc agcatttttt cgcgcggcat caacagctgt
     4081 gcattgtttt tcaccttcac ctcctttgtg gtgctcgcat cctgtcatag ccgttccgtt
     4141 aaatcgacgg ccagttttca atcctggaaa gagcctcgca aagtgaatct caggtgaagg
     4201 cacgacaaca aaagtattgt gataaaatcg ccaacttctg gtgtcaccca acaggaaaaa
     4261 tcatcatgag cctggccggt aaaaaaatcg ttctcggcgt tagcggcggt attgctgcct
     4321 ataaaacccc tgaactggtg cgtcgtttgc gcgatcgcgg ggccgacgtc cgcgtagcca
     4381 tgaccgaagc ggcaaaagcc tttatcaccc cacttagctt gcaggcggtt tctggttatc
     4441 ccgtttccga cagtctgctt gacccggcag ccgaagccgc tatgggccat attgagctgg
     4501 gtaaatgggc tgatttagtg attctcgccc ctgccacggc agatttgatt gcccgtgttg
     4561 ctgccggaat ggcgaatgac ctggtatcga cgatttgtct ggctacacct gcgcctgtag
     4621 ccgtgctccc cgccatgaac cagcagatgt accgtgccgc tgccacgcag cataatttag
     4681 aggtgcttgc ttcccgtggt ttgctcatct gggggccaga cagtggcagt caggcttgtg
     4741 gtgatatcgg tcctggacga atgctcgatc cgttaaccat tgtggatatg gcggtggcgc
     4801 atttttcgcc cgtcaacgac ctgaaacatc tgaacattat gattaccgcc ggtccgacgc
     4861 gtgaaccgct cgatccggtg cgttatatct ctaatcacag ctccggcaag atgggttttg
     4921 ctatcgccgc cgctgctgcc cgtcgtggcg cgaacgtcac gctggtatca ggtccagttt
     4981 cactaccgac gccaccgttt gttaaacgtg ttgatgtgat gaccgcgctg gaaatggaag
     5041 ccgccgtgaa tgcttctgta cagcagcaaa atatttttat cggttgcgcc gccgtggcgg
     5101 attatcgcgc agctaccgtg gccccagaaa aaatcaaaaa gcaggccacg cagggtgatg
     5161 aattaacaat aaaaatggtt aaaaaccccg atatcgtcgc aggcgttgcc gcactaaaag
     5221 accatcgacc ctacgtcgtt ggatttgccg ccgaaacaaa taatgtggaa gaatacgccc
     5281 ggcaaaaacg tatccgtaaa aaccttgatc tgatctgcgc gaacgatgtt tcccagccaa
     5341 ctcaaggatt taacagcgac aacaacgcat tacacctttt ctggcaggac ggagataaag
     5401 tcttaccgct tgagcgcaaa gagctccttg gccaattatt actcgacgag atcgtgaccc
     5461 gttatgatga aaaaaatcga cgttaagatt ctggacccgc gcgttaggaa ggaatttccg
     5521 ctcccgactt atgccacctc tggctctgcc ggacttgacc tgcgtgcctg tctcgacgac
     5581 gccgtagaac tggctccggg tgacactacg ctggttccga ccgggctggc gattcatatt
     5641 gccgatcctt cactggcggc aatgatgctg ccgcgctccg gattgggaca taagcacggt
     5701 atcgtgcttg gtaacctggt aggattgatc gattccgact atcagggcca gttgatgatt
     5761 tccgtgtgga accgtggtca ggacagcttc accattcaac ctggcgaacg catcgcccag
     5821 atgatttttg ttccggtagt acaggctgaa tttaatctgg tggaagattt cgacgccacc
     5881 gaccgcggtg aaggcggctt tggtcactct ggtcgtcagt aacacatacg catccgaata
     5941 acgtcataac atagccgcaa acatttcgtt tgcggtcata gcgtgggtgc cgcctggcaa
     6001 gtgcttattt tcaggggtat tttgtaacat ggcagaaaaa caaactgcga aaaggaaccg
     6061 tcgcgaggaa atacttcagt ctctggcgct gatgctggaa tccagcgatg gaagccaacg
     6121 tatcacgacg gcaaaactgg ccgcctctgt cggcgtttcc gaagcggcac tgtatcgcca
     6181 cttccccagt aagacccgca tgttcgatag cctgattgag tttatcgaag atagcctgat
     6241 tactcgcatc aacctgattc tgaaagatga gaaagacacc acagcgcgcc tgcgtctgat
     6301 tgtgttgctg cttctcggtt ttggtgagcg taatcctggc ctgacccgca tcctcactgg
     6361 tcatgcgcta atgtttgaac aggatcgcct gcaagggcgc atcaaccagc tgttcgagcg
     6421 tattgaagcg cagctgcgcc aggtattgcg tgaaaagaga atgcgtgagg gtgaaggtta
     6481 caccaccgat gaaaccctgc tggcaagcca gatcctggcc ttctgtgaag gtatgctgtc
     6541 acgttttgtc cgcagcgaat ttaaataccg cccgacggat gattttgacg cccgctggcc
     6601 gctaattgcc gcgcagttgc agtaatatga cgccggatga cttttcatcc ggcgagtttc
     6661 tttaaacgcc aaactcttcg cgataggcct taaccgccgc cagatgttcc gccatttccg
     6721 gcttctcttc cagataggca atcagatctt tcagggtgat gatagagatc actttgcagt
     6781 tgtaatcacg ctcaacttcc tgaatcgccg aaatctcgcc gcgcccgcgt tcctgacgat
     6841 cgagcgaaat caacacgcca gcaagcgtcg cgccattggc ctgaataatc tccatcgact
     6901 cgcgaatcgc cgttccggcg gtgatcacat catctaccag cattacgcgt ccttgtaacg
     6961 cgctaccaac cagattgccg ccttcaccgt ggtcttttgc ttctttgcgg ttaaagcagt
     7021 acggcaggtc caggtcgtga tgctccgcca gtgccacagc ggttgtggtg gcaatcggga
     7081 tccctttgta agcagggcca aacagcagat cgaactcaat gccggaatcc accaacgctt
     7141 cagcgtaaaa acggcctaac agtgccagat cgcgcccggt attaaacagc ccggcgttga
     7201 agaaataggg gcttttgcgc ccggatttca gcgtaaactc gccaaacttt aacacctgct
     7261 tgctaagcgc aaattcaata aactggcgct gatatgattt catgccttcg ctcctcatct
     7321 tacttttcta cagacaaaaa aaaggcgact catcagtcgc cttaaaaatc agtttgccag
     7381 cgccgccttc tgcgtcgcta caatggattc gattccccct cgggccagcg ccaacaaggt
     7441 gagtagctct tcatgggtga acggctcgcc ttctgccgtc ccctgcactt caatgatgcg
     7501 cccgtcttcg gtcatcacta cgttcatgtc ggtctctgcg gctgagtctt caacgtattc
     7561 cagatcgcaa attgcttcac cgtttacaat accgacagaa actgcggcta ccatcccttt
     7621 catcgggttg gttttcagct tgccgttttc caccagcttc tgtagcgcat ctgccagcgc
     7681 cacgcaggca cccgtaatcg acgcggtacg agtgccacca tcagcctgaa gcacgtcgca
     7741 gtccagcgta atggtgaact cacccagcgc tttcaaatct actgccgcgc gaagagcacg
     7801 ggcgatcaga cgctggattt ccattgtgcg tccaccctgc ttacctttcg ccgcttcacg
     7861 agcgttacgg gtgtgggtag aacgtggcag catgccgtac tctgcggtga tccagccctg
     7921 gccctgacct ttcaggaagc gcggcacgcc ttcttcaata gaggcggtac acaatacttt
     7981 ggtatcgcca aattcgacca gcaccgagcc ttctgcatgt tttgtatagt tacgagtcag
     8041 ggtaacggga cgcacctgat tattgctacg gcctgctgga cgcatattga aatctccggc
     8101 ttgaaacaaa tgtggctgcg cattatacgg acttccggcg gttattccta tcctgacaag
     8161 gcatcgatgg ctataatcct tccacctctc cttttataaa caggaacgtc tatgatccgc
     8221 agtatgaccg cctacgcccg gcgtgaaatc aagggtgaat gggggagcgc aacctgggaa
     8281 atgcgctcgg taaaccagcg ttatctggaa acttactttc gtctgccgga gcagttccgt
     8341 agccttgaac ctgtcgttcg cgagcgtatt cgttctcgcc tgacgcgcgg taaagtggaa
     8401 tgtaccctgc gctatgagcc agatgttagc gcacaaggtg agctgatcct caatgaaaaa
     8461 ctggctaaac agctggtaac tgccgcgaac tgggtaaaaa tgaagagtga cgaaggggaa
     8521 atcaacccgg ttgatattct acgctggccg ggcgtgatgg cagcccagga gcaggatctc
     8581 gacgcgattg ccgctgaaat tctcgcggcg ttggatggta cgctggacga ctttatcgtc
     8641 gcgcgcgaaa ccgaaggtca ggcactgaaa gcattgatcg agcagcgtct ggaaggcgtc
     8701 accgccgaag tggtcaaagt ccgcgcccat atgccggaaa ttctgcaatg gcagcgtgag
     8761 cgtctggtcg cgaagctgga agatgctcag gttcagttgg aaaacaaccg tctggagcag
     8821 gagttggttc tgctggcaca acgaattgac gttgccgaag agctggaccg cctcgaagcg
     8881 catgttaaag agacgtacaa cattctgaag aaaaaagaag cggttggtcg tcgtctggac
     8941 tttatgatgc aggagttcaa ccgtgagtcg aacactcttg cgtcgaaatc tatcaatgcc
     9001 gaagtgacaa actccgccat cgagctgaaa gtgttgattg agcagatgcg cgagcagatt
     9061 cagaatattg agtaactcgt aacaccaatt ctgacttttc agccttcaca aatgccgccc
     9121 actcaaacag agcggcattt ttcttccccg caacttcaat tctgttttgc gtgcctgctc
     9181 cagattttgc gatgtttttt tgcccagcac actgagaacg tgagatactc acaactgtat
     9241 ataaatacag ttacagattt actttctttg caatcgatat cacatggagt gggcaatgaa
     9301 cgaacatcat caaccttttg aagagataaa actgattaat gcaaacggag cagaacaatg
     9361 gtcagcaaga caacttggga aactactggg ttattcagag tatcgtcact ttatacctgt
     9421 attaacgcgc gccaaagagg cctgtgagaa cagtggtcac acaattgatg accatttcga
     9481 ggagatcctc gatatggtca aaattggctc aaatgccaaa agagcattaa aagacatcgt
     9541 actctcccgc tatgcctgtt acctggtagt acaaaacggc gaccccgcga aaccggtcat
     9601 tgcggcaggg cagacttatt ttactatcca gacccgacgg caggagcttg ctgacgatga
     9661 agcatttaag caacttcgtg aagacgaaaa gcgtctgttt ctgagaaatg agttgaaaga
     9721 acataacaaa caattggttg aggcagcaca gcaagctggg gtagcgacag ctactgattt
     9781 cgccatattt cagaatcatg gttaccaggg gctgtatggt ggattagatc agaaagccat
     9841 ccaccaactg aaggggctga aaaagagtca gaagatcctg gatcatatgg gttcaacaga
     9901 actggcggct aatctctttc gagctaccca aacagaagaa aaactcaagc gggatagcgt
     9961 gaattcaaaa cagcaagcta ataccacaca ctttgacgtg ggtagcaagg tgaggcaaac
    10021 cattcaggaa cttggcggaa ccatacctga ggagttgccg accccgcagg tcagcatcaa
    10081 gcagttggaa aacagtgtaa aaattacaga gaagaaatag cagaaacgaa aaactctttt
    10141 tgttgattga gacacccgat gcgtaagcca aggtccaggt gcagtgaaca caatggctaa
    10201 acattgcacc tttcttttcc cccctcagtt ttaacctatt ttttcttatg cattttctca
    10261 gacaagaagt cagaagaatg catctacgct acagaaaata gcgatttcac ataactaaaa
    10321 gttatcaatt tcctctcacc ttaaaaaata tcaatcgtga caatgcgcac aaatcgctac
    10381 cctgccagac agatttttag ggagagaacc atgctgttac acattttgta tttggttggc
    10441 attactgccg aagccatgac gggggcgctg gcggccgggc gacggcgcat ggatacattt
    10501 ggcgtaatta ttattgcaac cgccaccgca attggcggag ggtcagtgcg cgatattctg
    10561 ctgggccact atccgctcgg ctgggtcaaa cacccggaat atgtgattat cgtcgctacc
    10621 gccgcagtac tcaccactat cgttgctcct gtaatgcctt acctgcgcaa agtgtttctg
    10681 gtgctcgatg cgctcggact ggtggtcttt tctatcatcg gcgcacaggt tgcactggat
    10741 atggggcacg gcccaattat tgccgttgtc gcggcggtaa ccaccggtgt atttggcggc
    10801 gttttgcgcg atatgttctg taaacgcatt ccgctggtct ttcaaaaaga gttgtatgcc
    10861 ggtgtctcat ttgcctccgc cgtgctgtac atcgcgctgc aacactatgt ttctaaccat
    10921 gatgtggtga ttatctccac cctggtattc ggcttttttg ctcgtttact ggcgctacgt
    10981 cttaagctgg gattaccggt cttttactac agccacgaag gtcactaagg ttcaaaacct
    11041 gtgatctgct gggcagccag ccaactgccc agcttcttga tttgcgcatt ttccttccat
    11101 tcaataacct gtctggcgcg cccagatcca gtccccggca gctgctgcca gaactgctcc
    11161 gtgctaaata aaagttgcga ccaggaccgt tcatcactgg cattaagcgc cgcccgggtt
    11221 agcggtattc ccattgccat cacccagcga gtaaaaggct ggttacgagc cagattaaac
    11281 tgatgccata actgcgcact tttacttttc gcgatccccg gcgtgttctg taattgctct
    11341 ggcgttaata aaagccagga aaagatatgc tcaaagcgat gagtctgatg cagcgcgcgc
    11401 caaccggcct caccaatgcc atccagcccc agaacctgtt ttgaccccag ccagactaag
    11461 cgtgaaatga actgttcctg acaaacatca gaagcaaagt agcaggtcaa cgggttaaag
    11521 cggttttctg gtggtgtcgg ttttgtacgt tctgcaccgc gccagaccac atcatcaatg
    11581 cgaggaatac cctgaccggc aaggctgacg aggatctgat caccaggcgc aatatcccac
    11641 tcctgccagc gcctgacgga accaatattc acccgctgga cttttttatc atccagcatg
    11701 acaggtgcga gtgacgcaac caccgatatt ttaccgctct tacccaccgc aaactgaatc
    11761 gccttcactt cggcaacctg agctacaggt tgatatttcc aggccaccag ccactccgcc
    11821 tggcccggta gccaatgacg ggattcgggt tccttagccc ctcgcacaac cacgccatcg
    11881 gtgacgaagg gtaatttcgc tttccaccac tcattgcgta cgcgcgcaac ctcatcagca
    11941 tttttaaccg cacgggtata cctctgcgtt agagtaaaac ctgcggtagc cagctccttt
    12001 aaacgatcgg tcattaactg cggtccatcc ggccatgccc agacaaaaac agccagtgag
    12061 tttagcgtgt cgctattccc ctggcgcatc atcaggccag caacttttgc gcgggcattt
    12121 attcccccca tttgttgttg gatatgcccc tcgcgctgga gaaatatttc cccctgaagc
    12181 gtactgttgg ctaaaggccc gctaacggtt tgcagcacag cggaaattaa gctaactttc
    12241 tgcgtccagt cctcgccttt caggccatta ccgcgactga ttgctttgtt cagtttcccg
    12301 tcccgataaa ccagggttac cgcaacgcca tcaacttttg gctgcaccca aagatcgcta
    12361 cgttctcgca tccacagact taatgcgttt ttatccgcca ttttgcgtac acccgtatgg
    12421 gcaaccggat gcataaccgc accatttaaa ggcggcatca tcacgtcgcg gggctcgctc
    12481 acaaagcagc gttgccactg cgtaagacgg gcgcttaact gatcgtaaac accgtcttcc
    12541 acctcacttt ttccttcctt ccagtagtca tcgtcccact gttttatttg ctgttgcagg
    12601 cgggaaattt cttcctgtgc tctggctggc gaccaggccg gacagaccgc ccacacagat
    12661 gattgccagc acaagatact tattaatatc gccatccata ctttcatctt cacctccgct
    12721 gtagtcaggc tggcagatat acaacgtggt gaaggcaaag ccgagtggca aaaacggagt
    12781 ctgcgaggac gcttcctgag aatcgtcttt attgcagtaa atcacaggca aatgcggaaa
    12841 cagctacgca aaacgcaaca actttgcgca aaaagtgtga gcaagggcta cgtcacatgg
    12901 ccgcgccgtg tataataagc tcgtatgtag gctttatttc gctaatcaca tacgaaagat
    12961 actcatggct caaggcacgc tttatattgt ttctgccccc agtggcgcgg gtaaatccag
    13021 cctgattcag gctttattaa aaacccaacc gttgtatgac acccaggttt ctgtttcaca
    13081 caccacacgc caaccgcgtc ctggtgaagt ccacggtgaa cattatttct ttgttaatca
    13141 tgatgaattt aaagaaatga ttagcagaga tgcgttcctc gaacacgcag aagtttttgg
    13201 taattactat ggcacttcgc gtgaggccat tgagcaagta ctggcgaccg gtgtcgatgt
    13261 ttttctcgat atcgactggc agggcgcgca gcaaattcgc cagaagatgc cgcacgcgcg
    13321 gagtatcttt attttaccgc cgtccaaaat tgaactggac cgccgtctac gcggtcgcgg
    13381 tcaggacagc gaagaggtca ttgcaaagcg tatggcgcaa gctgttgcag aaatgagcca
    13441 ttacgccgaa tatgattatc tgattgtgaa tgatgacttc gataccgcgt tgaccgattt
    13501 gaagaccatt attcgcgccg aacgtctgcg catgagccgc caaaagcagc gtcatgacgc
    13561 tttaatcagc aaattgttgg cagactgaac ctgatttcag tatcatgccc agtcatttct
    13621 tcacctgtgg agctttttaa gtatggcacg cgtaactgtt caggacgctg tagagaaaat
    13681 tggtaaccgt tttgacctgg tactggtcgc cgcgcgtcgc gctcgtcaga tgcaggtagg
    13741 cggaaaggat ccgctggtac cggaagaaaa cgataaaacc actgtaatcg cgctgcgcga
    13801 aatcgaagaa ggtctgatca acaaccagat cctcgacgtt cgcgaacgcc aggaacagca
    13861 agagctggaa gccgctgaat tacaagccgt taccgctatt gctgaaggtc gtcgttaatc
    13921 acaaagcggg tcgcccttgt atctgtttga aagcctgaat caactgattc aaacctacct
    13981 gccggaagac caaatcaagc gtctgcggca ggcgtatctc gttgcacgtg atgctcacga
    14041 ggggcaaaca cgttcaagcg gtgaacccta tatcacgcac ccggtagcgg ttgcctgcat
    14101 tctggccgag atgaaactcg actatgaaac gctgatggcg gcgctgctgc atgacgtgat
    14161 tgaagatact cccgccacct accaggatat ggaacagctt tttggtaaaa gcgtcgccga
    14221 gctggtagag ggggtgtcga aacttgataa actcaagttc cgcgataaga aagaggcgca
    14281 ggccgaaaac tttcgcaaga tgattatggc gatggtgcag gatatccgcg tcatcctcat
    14341 caaacttgcc gaccgtaccc acaacatgcg cacgctgggc tcacttcgcc cggacaaacg
    14401 tcgccgcatc gcccgtgaaa ctctcgaaat ttacagcccg ctggcgcacc gtttaggtat
    14461 ccaccacatt aaaaccgaac tcgaagagct gggttttgag gcgctgtatc ccaaccgtta
    14521 tcgcgtaatc aaagaagtgg tgaaagccgc gcgcggcaac cgtaaagaga tgatccaaaa
    14581 aatcctctcg gaaatcgaag ggcgtttgca ggaagcggga attccctgcc gcgtcagtgg
    14641 tcgcgagaag catctttatt cgatttactg caaaatggtg ctcaaagagc agcgttttca
    14701 ctcaatcatg gacatctacg ctttccgcgt gatcgtcaat gattccgaca cctgttatcg
    14761 cgtgctcggg caaatgcata gcctgtacaa gccgcgtccg ggtcgcgtga aagactacat
    14821 cgccattcca aaagcgaacg gctatcagtc tttgcacacc tcgatgatcg gcccgcacgg
    14881 tgtgccggtt gaggtccaga tccgtaccga agatatggat cagatggcgg agatgggtgt
    14941 tgccgcgcac tgggcttata aagagcacgg cgaaaccagc accaccgcgc aaatccgcgc
    15001 ccagcgctgg atgcaaagcc tgctggagct gcaacagagc gccggtagtt cgtttgaatt
    15061 tatcgagagc gttaaatccg atctcttccc ggatgagatt tacgttttca caccggaagg
    15121 gcgaattgtc gagctgcctg ccggtgcaac gcccgtcgac ttcgcttatg cagtgcatac
    15181 cgatatcggt catgcctgcg tgggcgcacg cgttgaccgc cagccttacc cgctgtcgca
    15241 gccgcttacc agcggtcaaa ccgttgaaat cattaccgca ccgggcgctc gcccgaatgc
    15301 cgcgtggctg aactttgtcg ttagctcaaa agcgcgcgcc aaaattcgtc agttgctgaa
    15361 aaacctcaag cgtgatgatt ctgtaagcct gggccgtcgt ctgctcaacc atgctttggg
    15421 tggtagccgt aagctgaatg aaatcccgca ggaaaatatt cagcgcgagc tggatcgcat
    15481 gaagctggca acgcttgacg atctgctggc agaaatcgga cttggtaacg caatgagcgt
    15541 ggtggtcgcg aaaaatctgc aacatgggga cgcctccatt ccaccggcaa cccaaagcca
    15601 cggacatctg cccattaaag gtgccgatgg cgtactgatc acctttgcga aatgctgccg
    15661 ccctattcct ggcgacccga ttatcgccca cgtcagcccc ggtaaaggtc tggtgatcca
    15721 ccatgaatcc tgccgtaata tccgtggcta ccagaaagag ccagagaagt ttatgggtgt
    15781 ggaatgggat aaagagacgg cgcaggagtt catcaccgaa atcaaggtgg agatgttcaa
    15841 tcatcagggc gcgctggcaa acctgacggc ggcaattaac accacgactt cgaatattca
    15901 aagtttgaat acggaagaga aagatggtcg cgtctacagc gcctttattc gtctgaccgc
    15961 tcgtgaccgt gtgcatctgg cgaatatcat gcgcaaaatc cgcgtgatgc cagacgtgat
    16021 taaagtcacc cgaaaccgaa attaatgttt tatgaaccca acacgttatg cacgcatctg
    16081 cgaaatgctc gccaggcggc agcctgatct gaccgtctgc atggagcagg tccacaaacc
    16141 tcataacgtt tctgcgatta ttcgtaccgc agatgccgtt ggcgtacatg aagttcacgc
    16201 cgtctggcct ggtagccgca tgcgcaccat ggcttcggca gcggcgggta gtaacagctg
    16261 ggtacaggtg aaaacacacc gcaccattgg cgatgccgtc gctcatctca aaggccaggg
    16321 catgcagatt ctggcaaccc atctttctga taatgctgtc gatttccgcg aaattgatta
    16381 cactcgcccg acctgcattt tgatgggaca ggagaaaacg ggcatcacgc aggaagcatt
    16441 ggccctggcg gatcaggaca tcatcattcc gatgatcggc atggtgcagt cgctgaatgt
    16501 ttccgttgcc tcagccctca ttctttacga agcccagcgt cagcggcaaa atgcaggcat
    16561 gtacctgcgt gaaaacagca tgttgccgga agcagagcaa caacgcctgt tgtttgaagg
    16621 cggctatccg gtgctggcga aagtcgcaaa acgcaaaggc ctgccttatc cccacgtcaa
    16681 tcagcaaggc gagatcgaag ctgatgccga ctggtgggct actatgcagg ctgcagggta
    16741 agtgccatga aaggtcgcct gttagatgct gtcccactca gttccctaac gggcgttggc
    16801 gcagcactta gtaacaaact ggcgaaaatc aacctgcata ccgtgcagga tctactctta
    16861 caccttcccc tgcgctacga agatcgcacc catctctacc ccatcggaga actactgccg
    16921 ggcgtttatg ccacggtgga aggcgaagtg ctgaactgca atatctcctt cggcggtcgg
    16981 cggatgatga cctgccagat cagcgacggt tccggcatcc tcaccatgcg ctttttcaat
    17041 ttcagcgcgg caatgaaaaa tagcctggcg acgggccgcc gtgtactggc ttatggcgaa
    17101 gcaaagcgcg gtaaatatgg tgcggagatg atccacccgg aataccgcgt gcagggcgat
    17161 ctcagcacgc cagaattaca ggaaacgctc acgccggttt atccaacaac ggaaggcgta
    17221 aagcaggcca cgctgcgtaa attaaccgac caggcgctgg atctgctcga cacctgcgcc
    17281 attgaagaac tcctgccgcc ggaactgtca caaggaatga tgacgctacc ggaagcgttg
    17341 cgcactttgc accgcccgcc accgacgcta cagcttagcg atctggaaac cgggcagcat
    17401 ccggcgcaac gtcgtctgat tctggaagaa ctgctggcgc acaacctcag catgttagcc
    17461 ttacgtgccg gagcacagcg ttttcatgcc cagccgctga gcgccaatga cacgctgaaa
    17521 aataaactcc tcgccgcctt accgttcaag ccaacgggcg cacaggcacg cgtagtggcg
    17581 gagatcgagc atgatatggc gctggatgtg ccgatgatgc gcctggtaca gggcgatgta
    17641 ggttccggta aaacgctggt cgccgccctc gccgcgctgc gtgcgattgc ccacggcaaa
    17701 caggtggcac tgatggcacc aaccgaatta ctcgccgagc agcacgccaa taacttccgc
    17761 aactggtttg caccgctcgg tatcgaagtg ggctggctcg ccggtaagca gaaaggcaaa
    17821 gcgcgactgt ctcagcagga agccatcgcc agcggtcagg tgcagatgat tgtcggtaca
    17881 cacgccatct tccaggaaca ggtgcagttt aacggcctgg cgctggtgat tatcgacgaa
    17941 cagcatcgtt ttggcgtgca tcagcgtctg gcattgtggg agaaaggcca gcagcagggc
    18001 ttccatccgc atcagttgat catgaccgcc acgccgatcc cccgcacgct ggcaatgacg
    18061 gcgtatgccg atctcgatac ctcggtgata gatgagctgc cgccaggccg cacgccagtg
    18121 actacggtcg ctattcctga tacccgccgt accgacatca ttgaccgcgt gcgccacgcc
    18181 tgcataactg aaggtcgtca ggcgtactgg gtttgtacgt tgattgaaga gtcagagtta
    18241 ctggaagcgc aggcggcgga agctacctgg gaagagttga aactggcgct accagaattg
    18301 aatgttggcc tggtacacgg gcggatgaaa ccagccgaga aacaggcggt gatggcgtcg
    18361 tttaaacaag gtgagctaca tctgctggtt gccactaccg ttattgaagt cggtgttgat
    18421 gtgcctaacg ccagcctgat gattatcgaa aaccctgagc gtctgggtct ggcgcagtta
    18481 caccagctgc gcgggcgcgt aggtcgtggc gcggtggctt ctcactgcgt gctgctctac
    18541 aaaacgccgc tttctaaaac ggcgcaaatt cgcctgcaag tgctgcgcga cagtaacgac
    18601 ggttttgtga ttgcgcaaaa agatctggag attcgcggcc ctggcgaatt gttaggcacg
    18661 cgtcagacgg gtaatgctga atttaaagtg gcggatttac tgcgcgatca ggcgatgatc
    18721 ccggaagttc agcgcctggc gcgacatatt cacgaacgtt acccacaaca ggcaaaagcc
    18781 ttgatagaac gctggatgcc ggagacagaa cgttactcga atgcgtaaaa ggcggcggtg
    18841 agaagaccgc cgcttcatcg gttaaccggc aaaaatcggc aacattaaat acagcttaat
    18901 caccagcgca ttgacgatat cgataaagaa cgcaccgacc atcggcacca ccaaaaacgc
    18961 catgtgcgac gggccaaagc gttcagtgat cgcctgcatg ttggcgattg ccgttggcgt
    19021 tgcaccgagg ccaaaaccac agtgacccgc agccagcact gccgcatcgt agtttttgcc
    19081 catcatgcgc caggtaacga agatggcata caacgccatg aagatggtct gtaccaccag
    19141 aatcgccagc atcggcagcg ccagcgaagc cagctcccac agtttcagcc ccatcaacgc
    19201 catcgccagg aacaacgaca agcttacgtt acccagcacg gataccgcac gctcaaagac
    19261 gcggtaaaag cccattattg acagaccgtt gctcagaatc acgccaacaa acagtacaca
    19321 gacgaaggtc ggcagttcaa aagcagtgcc agccaaaagt tgcgcaacaa ttttccccac
    19381 cgtcaggcag atagcaatca gcgcgatagt ttcaatcagc accaacgagg tgatcatgcg
    19441 tcccacatcc ggcttttcaa acgccgtcgg gacttcctgg tcatccggaa taccgttcgg
    19501 cgtggtggag tgtttcacca gatagcgcgc caccggaccg ccaatcaagc cgcccagcac
    19561 cagaccgaac gttgcacagg ccatcgccac ttccgtcgca ttggtgaagc cataacgttc
    19621 aatgaacaat ttactccacg cagcgcccgt accgtgaccg ccggaaagcg taatagaacc
    19681 ggccaacagc cccatcagcg gatcaagccc taacaggcta gccataccaa tgccaatggc
    19741 attttgcatc accaacagac caacaaccac aatcaagaag atgccaacca cacgcccacc
    19801 ggcacgcaaa ctggcaatgt tggcgttcag gccaatggtg gcgaagaaag ccagcattaa
    19861 cggatcgcgc agggacatat caaagttgac ttcccagccc atgctttttt tcagtactag
    19921 tagcgccagc gccaccaaca aaccacccgc aacaggttcc ggtatggtgt atttcttcaa
    19981 aaaggagacg gaatggacca acttacgccc gagcagcaac gtcagcgttg cggcaacaag
    20041 cgttgctaaa gtatcgagat gaaacatagt tactcctttg tatccgcatc actctttcat
    20101 actcgtcata cttcatcgcg cagtggcaac ttcatgaact atgacgggta tcgagttatg
    20161 ctttcctggg tcaaattctc agctgttaat gaacaaatgg catggatttt aagcagaaaa
    20221 ataccaaaag ttatataaaa aagtgcattt atctcattta aatatttaga tgaggatgtg
    20281 gcaaacgttt gcttttctct ttcggtcagc taaaatgccc gctttgctac cacgggattg
    20341 ttttcgatgt ctgtttccac cctcgagtca gaaaatgcgc aaccggttgc tcagactcaa
    20401 aacagcgaac tgatttaccg tcttgaagat cgtccgccgc ttcctcaaac cctgtttgcc
    20461 gcctgtcagc atctgctggc gatgttcgtt gcggtgatca cgccagcgct attaatctgc
    20521 caggcgctgg gtttaccggc acaagacacg caacacatta ttagtatgtc gctgtttgcc
    20581 tccggtgtgg catcgattat tcaaattaag gcctggggtc cggttggctc cgggctgttg
    20641 tctattcagg gcaccagctt caactttgtt gccccgctga ttatgggcgg taccgcgctg
    20701 aaaaccggtg gtgctgatgt tcctaccatg atggcggctt tgttcggcac gttgatgctg
    20761 gcaagttgca ccgagatggt gatctcccgc gttctgcatc tggcgcgccg cattattacg
    20821 ccgctggttt ctggcgttgt ggtgatgatt atcggcctgt cgctaattca ggtggggctg
    20881 acctccattg gcggcggtta cgcagccatg agcgataaca ccttcggcgc accgaaaaat
    20941 ctgctgctgg caggcgtggt tttagcctta attatcctgc ttaaccgtca acgtaaccct
    21001 tacttacgcg tggcctcact ggtaattgcg atggcggccg gatatgcgct ggcgtggttt
    21061 atgggcatgt tgccagaaag caacgaaccg atgacgcaag aactgattat ggtgccaacg
    21121 ccgctctatt acggtcttgg cattgaatgg agtctgctgc tgccgctgat gctggtcttt
    21181 atgatcactt cgctggaaac cattggcgat atcacggcga cctctgacgt ttccgaacag
    21241 ccagtgtccg gtccgctgta catgaaacgc ctgaaaggcg gcgtgctggc aaacggcctg
    21301 aactcgtttg tttcggcggt gtttaacacc ttcccgaact cctgcttcgg gcaaaacaac
    21361 ggagtgatcc agttgactgg tgttgccagc cgctatgtcg gttttgtcgt cgcgctgatg
    21421 ttgatcgtgc tgggtctgtt cccggcagtg agcggttttg tacaacacat tccagaaccg
    21481 gttctgggcg gcgcaacgct tgtaatgttt ggcaccatcg ccgcctccgg tgtgcgtatc
    21541 gtttctcgtg agccgctgaa ccgtcgggcg attctgatta tcgcgctgtc gctggcggtt
    21601 ggtctgggcg tgtctcagca gccgctgatt ttgcagtttg cccctgaatg gctgaaaaac
    21661 ctgctctcct ccgggatcgc cgcgggcggt attactgcca tcgtgctgaa tctgattttc
    21721 ccaccagaaa aacagtaatt cactggcggc ggtaagataa ttaccgccgc agtctcacct
    21781 ctttcaccat tcccgccttg aggattgcgc gtaaatcgtg cataactccc ttatgtgcat
    21841 ttcacgggat ggaagaccat gaaatttatt gggaagctgc ttctctacat tctcatcgct
    21901 ctgttagtgg tgatcgctgg cctctatttt cttctgcaaa cccgctgggg agcagaacat
    21961 atcagcgcat gggtttccga gaatagcgac tatcatctgg ccttcggggc gatggatcac
    22021 cgtttttccg cgccatctca tatcgtgctg gagaatgtca cgtttggtcg tgatggtcag
    22081 cccgcgcccc tggtggcaaa aagtgtcgac attgcgctaa gcagtcgcca actgaccgaa
    22141 ccacgccatg tcgataccat cctgctggaa aacgggacgc tgaatctcac cgaccagacc
    22201 gcgccgctac cgttcaaagc cgatcgtctg caactgcgtg atatggcgtt taatagcccg
    22261 aatagcgaat ggaaactgag cgcgcagcgg gtaaatggcg gcgtggttcc gtggtcacca
    22321 gaagccggta aagtgctggg tacgaaggcg cagattcagt ttagtgccgg atcgctttcg
    22381 ctcaatgatg ttcctgccac caatgtactg attgaaggca gtattgataa cgatcgcgtt
    22441 acgctgacta acctgggtgc cgacatcgcc cgcgggacat taaccggaaa cgcgcagcgt
    22501 aacgccgacg gcagctggca agtggaaaat ctgcgcatgg cggatatccg tctacaaagc
    22561 gaaaaatcgc taaccgactt ctttgcgcca ttacgctctg tcccgtcgtt gcagattggt
    22621 cgcttggaag tcatcgatgc tcgtctgcaa ggtccggact gggcggtgac cgacctcgat
    22681 ctcagcttgc gcaacatgac cttcagtaaa gatgactggc agacacagga aggcaaactg
    22741 tcgatgaacg ccagcgagtt catttatggt tcgctgcatt tatttgaccc gattataaac
    22801 gcggaatttt ccccgcaggg cgtagcgctg cgccagttca ccagccgctg ggaagggggt
    22861 atggtcagaa cgtcagggaa ctggctgcgt gacggtaaaa cgttgatcct tgatgatgcg
    22921 gcaattgccg ggctggaata taccttgccg aaaaactggc aacagttgtg gatggaaacg
    22981 acacccggtt ggttaaacag cctgcaactg aagagattta gcgccagccg caatctgatc
    23041 attgatatcg accctgactt cccgtggcag ctcaccgcgc tcgatggtta cggtgccaac
    23101 ctgacgctgg ttaccgatca taaatggggc gtctggagtg gctcggcgaa tctgaatgcc
    23161 gccgccgcga cattcaatcg tgttgatgtt cgtcgcccgt cgctggcgct gaccgccaac
    23221 agcagcacgg tgaatatcag cgaactgagt gcatttactg aaaaaggcat tctggaagcc
    23281 accgcaagtg tttcacaaac gccacaacgt cagacccata tcagcctgaa tggacgcggt
    23341 gtgccggtga atattttgca acaatgggga tggcctgaat taccgttgac tggcgacggc
    23401 aatattcagc ttaccgccag tggagatatt caggccaatg ccccgctgaa acctacggtt
    23461 agcgggcaat tgcatgccgt gaacgccgca aagcagcaag tgactcaaac catgaatgcg
    23521 ggcgtcgttt ccagcggtga agttacatcg acggagccgg tgcggtaaac atcaatctcg
    23581 ccgggtacga ctcagacgta cccggcattc cgtcaataga ttacaacgta attgtcagcg
    23641 cattcccttg aggcgtcacc accagcccct gctcactttc agcctgcgaa ccgccttgca
    23701 gaccatttac tttcacaaca ttgcgcaagc acagcgtcca gttcttcgcc tcgcccgcac
    23761 cagtcacagt aatcgtgttg ccagtacgtg ctgcttttaa agtaaagatc accgatccgt
    23821 cagcagcagg cacttcacag acggcttcat gcccgtcttg cagattaaag aggtggaatg
    23881 ccgtgccttc gtgccacacg taatcgggac gttgatcgtt gttgcccagc gccagtagag
    23941 tgttatcacg cacataaacg ggcagactca ggaagccgtg ctgctgttta tgccagcgac
    24001 taccgtcgag ttcatcgttg tgccacaggt gtgtccagcg accttccggc aggtagaact
    24061 gcacatcgcc cgcttcagtg aacaccggcg caaccatcac gttgtcgcct aacatgtatt
    24121 gacggtcaag gtaatcacaa gccggatcgt ccgggaactc catcatcatg gcccgcatca
    24181 tcggcgtacc ccgcgcgttc gcacgcgcag cttcacgata cagatacggc atcatgcggc
    24241 atttcagttg cgtgaagaag cgcaccacat cacaggactc atcatcgtag gcccacggca
    24301 cacgataaga tttgctaccg tgtaaacggc tatggctgga gagcaaacca aacgcgcacc
    24361 agcgtttgta aacgtgcgcc ggagcggtat tttcaaagcc gccgatatcg tggctccaga
    24421 agccaaaacc tgaaaggcca atagacaaac caccgcgcag gctttccgcc attgattcgt
    24481 agttagcgta acaatcgcca ccccagtgta ccgggaattt ctgcgcaccg acggaggccg
    24541 agcgggcaaa caagacagct tcttcctcac caacggtgtc cttgagcacg ttccacacca
    24601 gttcgttgta gatgtacgca taatggttat gcattttctg cgaatcggaa ccgtcaaacc
    24661 actgaacatc aattgggata cgttcgccaa agtcggtctt aaagcaatca acgcccatcg
    24721 cgaccagacc tttcagtttg tcggcgtacc atttgcaggc atccggattg gtaaagtcat
    24781 aaatcgccag acctggctgc catttatccc actgccatag cgaaccgtcc gggcgtttga
    24841 gtaaatagcc tttctcttgt aactctttaa agacggggga tttttgaccg atataggggt
    24901 taatccagac gcagattttc agccctttcg ctttcaggcg gcggatcatc ccttccgggt
    24961 cagggaaagt cagcgggtcc cactcaaaat cgcaccactg gaaggctttc atccagaaac
    25021 agtcaaagtg gaaaacatgc agcggcagat tgcgttccgc cataccatcg ataaagctgt
    25081 ttaccgtcgc ttcgtcgtag ttggtggtaa atgaagtggt tagccacagg ccgaaggacc
    25141 acgggggcgg cagcgccgga cgaccagtaa agcgggtata acgatcaagt accgctttcg
    25201 gcgtcgggcc gtcgataaca aagtattcga gatattcact ctcaacgctg aactgcactt
    25261 tggagacttt ctccgatccc acttcaaaag agacacactg gggatgattg accagtaccc
    25321 cataaccacg gttagtcatg tagaacggga tatttttata cgcctgttca gtacttgtgc
    25381 cgccgtcccg gttccaggtc tctaccgtct ggccattgcg caccagggca gtaaagcgct
    25441 ctcccagacc gtaaactgtt tcgccaacgc caagatcaag ccgctcaaac atataattgc
    25501 gttgattatt cgtgtcctgc acgtagccat tatttttcac ctgactaccg gtaatacgtt
    25561 cgccgttgcg cagaaaatcc agtgaccaga actcaccttt gctgacacgc gcgcttaagt
    25621 tgccactttt aaactcagca taacgttctg tgttttcgat tgtgaccttc acgtcctgca
    25681 aaatattgag cggataatga ggaccgttat tcagcgcccc ctgaaaatgc tcaatccgca
    25741 caccgacaat accttcctgt ggggagaaaa agcgcagcgt aaataaaggc gtatcaagct
    25801 gccaggtacg ttcacgcaca tcacgggggg cagcatagac caccatttca ttatcctgct
    25861 gttcaacctc gaatacctga agcgggtgaa tcaaattgag gccaggttga atcaaccagt
    25921 ttccatcgct aattttcatt gccgttcctt agttctgcac ttcttgagag gtgaattgtt
    25981 gctggcaacg gcgtttaccc tgagccagct gttccataac ggttttcaga ttgtgcgtcg
    26041 tgagtgagta gtagcgttta gcgataatcg cgctcagcaa ataacagatc gccggaacaa
    26101 tcgtgaatag cgcaataatg atgctaatcg tggcgctgtt ctgcgctttt tctgccgcat
    26161 catatccgcc ataagccagc atccagccga taagagcgcc gccgaaggcc aaccccagtt
    26221 tgagcacaaa cagcgtgcca gcaaaactga tcccggtcag gcgtttacca ttgcaccact
    26281 cgccgtagtc gacggtatcg aacatcatta cccactggat aggtgtcacc agttgatgca
    26341 acacaccaat cacgaagatg aagacaaaca tagtgatgct ggcctgcatg ggaacaaaga
    26401 acatcgcgag gctaatcact gccagcaggg cgttcgtcca ccagaagata gtgactttac
    26461 atttccagtc ggtcagaggt tttgccagtg cggaaccaat caggttaccc acgcaataag
    26521 tggtgagaaa agcgacaaac acttccggcg tgcccaaaat ccatgtgacg taatacatca
    26581 tcgccccacc gcgtacgcac accgccagga tattgaaaat ggttagtaaa ccgacaatcc
    26641 gccactggtc gttttgccag atatcacgta aatcttcccg catagacgtt gttgtaggtg
    26701 gtgcttcaac gcgttcttta gtggtgaaga aacaaaatgc cagcatcatg aatgccacca
    26761 cggaaaggac cacgataccg ccctggaaac cgagtggttt attatcaccg ccaattaaat
    26821 taaccagtgg catcatcaga acagtagaaa gcatgcctcc cgcggtcgcc agcacaaaac
    26881 gccaggattg cagcgagata cgctgagtcg ggtcattggt gattacacca cccaatgcgc
    26941 agtaagggat attgacgacg gtataaagta aggtaagtta gggtgtaagt aattgctgca
    27001 tagatcattt tgccgttcat actgagatct ggcgtgctat aggccagtac acagacgatc
    27061 ccgaatggca gtgcgccaaa cagtacccac ggacgaaatt taccccagcg agagcgcgtt
    27121 cgatcggcca acaaccccat gcaaggatcg gaaatcgcat ccagtgcacg agcgaccaaa
    27181 aacatggttc cgacaaatcc ggcaggaatg ccaaaaatat cggtataaaa gaacatcata
    27241 tataacatta cgttatcgaa aataatgtgg ctggcggcgt ctcccatgcc ataaccaatt
    27301 ttctctttaa cggacaacac ttcactcctc atctgctttt ccttgatgct ataggggtgc
    27361 tgagaccgat aacgcatttt gtaaaccatc cctgtgagag cgaatattcc gatatctggt
    27421 tattaattta tgattcttgt tttatgtgat cgtggtagcg ttaattccgc tcatatatca
    27481 ttgtaaaata tgggttttat ataaattata atgctttcgt gataatacgc tgcgtttatt
    27541 aggcggaaaa aactgatctg ggggatgtag aaactcaagg aagtagctat aatgcgcccc
    27601 gcctccatgt agcaatcgag gcgcggaaga tcgtcgtctc cggtgaggcg gctggacttc
    27661 aaatccagtt ggggccgcca gcggtcccgg gcaggttcga ctcctgtgat cttccgccag
    27721 tttacctctt ctgaactctc ccaatgtcaa taaatctctt ctaatccctt gtatatcagt
    27781 atttatttgt atcctgaggt caacctactt caccccgtat caagtttctt ttgggggtac
    27841 tttagggggt gttatttcct attgaaatac aagcaccccc ataataggaa aaatgtgata
    27901 cataacaacg gcttttaaca atatctttgt tatacaaggg gtgtattggg ggtatcaggt
    27961 tagttactgg cagaaatccc cctcaggaac atgaattcat caaaagccgg aggatgtcat
    28021 ggcattaacg atatcaaagt gaaaaccgca aaacccaaag acaaacccta taagcttgct
    28081 gatggcggcg gtatgtatct actgattaac actaatggtt ctaaatactg gcgcatgaag
    28141 taccgctttg ctggtaaaga aaagatgctt tctattggcg tataccccga tgtaacgcta
    28201 gccgatgctc gcgagaaacg cagtgaagcc aggaaactcc tcgctgctgg aggcgatcca
    28261 ggggaggcaa aaaaggaaga aaaaatcgcg cagcaaatga gtctgaaaaa tacgttcgag
    28321 gctattgctc gcgaatggca ccagttaaaa gctgatcggt ggtcattacg ctatcgtgat
    28381 gaaatcattg atacctttga aaaagacatt tttccctaca ttggcaagcg ccctattgcc
    28441 gaaatcaaac caatgaagtt actggaagca ctgcgtaaaa tggaaaaacg tggagctctg
    28501 gagaaaatgc gcaaggtccg ccagcgctgt ggtgaggtgt tccgctatgc aattgttact
    28561 ggccgggcag actataaccc agcccccgat cttgccagtg ctttagctac accgaaaaaa
    28621 gtacattttc cctttcttac tgctaatgaa cttccccact tcctcaacga tctggcgggt
    28681 tataccggaa gtataatcac taagacagct actcagatca ttatgttaac cggcgtacga
    28741 acacaagaac tgcgttttgc acgctgggaa gatatcgatt ttgagacaaa attgtgggag
    28801 atcccggcag aagtaatgaa gatgaagcga cctcatattg tgccgctctc tgagcaggtc
    28861 attatgctat ttaagcagct tgaaccaatc tcaaaacatc atcccctagt ctttatcggt
    28921 agaaatgatc cccgcaaacc aattagtaag gaaagcatta accaagtaat tgaattactt
    28981 ggatataagg gaagactcac agggcacggt ttcagacaca ccatgagtac aattctgcat
    29041 gaacaaggct ttaattccgc ctggattgaa atgcagttag ctcatgtgga taaaaattcc
    29101 atcaggggta cctataatca tgccctgtat ctcgatggcc gccgtgaaat gatgcaatgg
    29161 tacgctgatt acattgattc actctccagc cgggaaagtt aagtcagaaa tacttttccc
    29221 tgatgttttc ctcaattaag tattttatcc tctggcctac aaggctggag gatttttgtt
    29281 attatattat ttttttgcgt cttcaaactt attacaaaaa cataattaaa aacaaattga
    29341 catttttaaa ttggcaatta caaaat
//
LOCUS       DAEBXN010000044        29043 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.044, whole genome shotgun sequence.
ACCESSION   DAEBXN010000044 DAEBXN010000000
VERSION     DAEBXN010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29043
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.044"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            109..1140
                     /gene="mdtN"
                     /locus_tag="KJL99_002007"
     CDS             109..1140
                     /gene="mdtN"
                     /locus_tag="KJL99_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HBD4436741.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMTTVRAPFDGR
                     IISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            1140..3182
                     /gene="mdtO"
                     /locus_tag="KJL99_002008"
     CDS             1140..3182
                     /gene="mdtO"
                     /locus_tag="KJL99_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HBD4436742.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIYGQTFPAMLDYPEVVVRLTLWCIVVG
                     LYPTLLMTLIGVLWFPNRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREALALQ
                     KLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKLASE
                     INKLQHAVAEGQCWQSDWRLSESEAVAARECNLENICQTLLQLGQMNPNTPPTPAAKP
                     PSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVGSSY
                     QKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERSSYI
                     GTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAMIYTFVWPESEARTLPQ
                     KLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLDSEE
                     RALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGLATA
                     LSRSPQITLEETPTSQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGATQ"
     gene            3179..4645
                     /gene="mdtP"
                     /locus_tag="KJL99_002009"
     CDS             3179..4645
                     /gene="mdtP"
                     /locus_tag="KJL99_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HBD4436743.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAEHVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            4843..5082
                     /locus_tag="KJL99_002010"
     CDS             4843..5082
                     /locus_tag="KJL99_002010"
                     /inference="COORDINATES: protein motif:HMM:NF016750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4436744.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVS"
     gene            5092..5262
                     /locus_tag="KJL99_002011"
     CDS             5092..5262
                     /locus_tag="KJL99_002011"
                     /inference="COORDINATES: protein motif:HMM:NF012602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBD4436745.1"
                     /translation="MNYVAERLSAIKEKYGPDAIQTTGSSRGTGNETNYVMQKFARAV
                     IGTNNVDCCARV"
     gene            5311..6723
                     /gene="fdhF"
                     /locus_tag="KJL99_002012"
     CDS             5311..6723
                     /gene="fdhF"
                     /locus_tag="KJL99_002012"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HBD4436746.1"
                     /translation="MSNAINEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIV
                     CDPRKIETARIADMHIALKNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKI
                     VEGYTPESVEDITGVSASEIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLA
                     MLTSNLGKPHAGVNPVRGQNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVES
                     LPAHTGYRISELPHRAAHGEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFI
                     TKTASAADVILPSTSWGEHEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMG
                     YPMHYNNTQEIWDELRHLCPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKF
                     DTPNGLAQFFTCDWVAPIDKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPG
                     YAQINTEDAKRLGIEDEALV"
     gene            6724..6990
                     /locus_tag="KJL99_002013"
     CDS             6724..6990
                     /locus_tag="KJL99_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HBD4436747.1"
                     /translation="MHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTESLSP
                     ITKTPEYKYCAVRVEPISDQRAAEQYVIDEYNKLKTRLREAALA"
     gene            7084..7773
                     /locus_tag="KJL99_002014"
     CDS             7084..7773
                     /locus_tag="KJL99_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HBD4436748.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(7851..9164)
                     /gene="gltP"
                     /locus_tag="KJL99_002015"
     CDS             complement(7851..9164)
                     /gene="gltP"
                     /locus_tag="KJL99_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HBD4436749.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVAGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(9506..10102)
                     /gene="nrfG"
                     /locus_tag="KJL99_002016"
     CDS             complement(9506..10102)
                     /gene="nrfG"
                     /locus_tag="KJL99_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HBD4436750.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(10099..10482)
                     /gene="nrfF"
                     /locus_tag="KJL99_002017"
     CDS             complement(10099..10482)
                     /gene="nrfF"
                     /locus_tag="KJL99_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HBD4436751.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            complement(10475..12193)
                     /locus_tag="KJL99_002018"
     CDS             complement(10475..12193)
                     /locus_tag="KJL99_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HBD4436752.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQSDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTRADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            complement(12213..13169)
                     /gene="nrfD"
                     /locus_tag="KJL99_002019"
     CDS             complement(12213..13169)
                     /gene="nrfD"
                     /locus_tag="KJL99_002019"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HBD4436753.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPSLPVLLLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(13166..13837)
                     /gene="nrfC"
                     /locus_tag="KJL99_002020"
     CDS             complement(13166..13837)
                     /gene="nrfC"
                     /locus_tag="KJL99_002020"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HBD4436754.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(13834..14400)
                     /gene="nrfB"
                     /locus_tag="KJL99_002021"
     CDS             complement(13834..14400)
                     /gene="nrfB"
                     /locus_tag="KJL99_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HBD4436755.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQASPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(14445..15881)
                     /gene="nrfA"
                     /locus_tag="KJL99_002022"
     CDS             complement(14445..15881)
                     /gene="nrfA"
                     /locus_tag="KJL99_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HBD4436756.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHHDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            16274..18232
                     /gene="acs"
                     /locus_tag="KJL99_002023"
                     /pseudo
     CDS             16274..18232
                     /gene="acs"
                     /locus_tag="KJL99_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
     gene            18432..18746
                     /locus_tag="KJL99_002024"
     CDS             18432..18746
                     /locus_tag="KJL99_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HBD4436757.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            18743..20392
                     /gene="actP"
                     /locus_tag="KJL99_002025"
     CDS             18743..20392
                     /gene="actP"
                     /locus_tag="KJL99_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HBD4436758.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTSADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVADLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            20570..21862
                     /locus_tag="KJL99_002026"
     CDS             20570..21862
                     /locus_tag="KJL99_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBD4436759.1"
                     /translation="MRYNGLNNMFFPLCQINDNHSITSSSHTKKTKSDNYSKHHKNTL
                     IDNKALSLFKIDDHEKVIGLIQKMKRIYDSLPSGKITKETDRKIHKYFIDIALYANNK
                     CDDRITRRVYLSKEKDVSIKVVYFINNVAVHNNTIEIPQIVNGGYDFSHLSLKGIVIK
                     DEDLSNSNFAGCRLQNAIFQDCNMYKTNFYYAIMEKILFDNCILDDSNFAQIKMADGT
                     LNACSAMHVQFYNAAMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPYVNLFKADLSF
                     SKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICTGSNMANVNF
                     NNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVT
                     LQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(22016..23665)
                     /locus_tag="KJL99_002027"
     CDS             complement(22016..23665)
                     /locus_tag="KJL99_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HBD4436760.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(23816..25165)
                     /gene="ghxP"
                     /locus_tag="KJL99_002028"
                     /gene_synonym="yjcD"
     CDS             complement(23816..25165)
                     /gene="ghxP"
                     /locus_tag="KJL99_002028"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HBD4436761.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLAGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(25871..26335)
                     /gene="soxR"
                     /locus_tag="KJL99_002029"
     CDS             complement(25871..26335)
                     /gene="soxR"
                     /locus_tag="KJL99_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HBD4436762.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            26421..26744
                     /gene="soxS"
                     /locus_tag="KJL99_002030"
     CDS             26421..26744
                     /gene="soxS"
                     /locus_tag="KJL99_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HBD4436763.1"
                     /translation="MSHQKIIQDLIAWIDVHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(26747..28324)
                     /gene="pdeC"
                     /locus_tag="KJL99_002031"
                     /pseudo
     CDS             complement(26747..28324)
                     /gene="pdeC"
                     /locus_tag="KJL99_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
     gene            28753..29034
                     /locus_tag="KJL99_002032"
     CDS             28753..29034
                     /locus_tag="KJL99_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HBD4436764.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
BASE COUNT         7283 a         7776 c         7353 g         6631 t
ORIGIN      
        1 cgggcaaata tcaatctggc atttgtcgga attatttata ccgccctttt tgctctctac
       61 gccatgctgt tctggctggc atttttctaa tctatttgag acgcgctcat ggaaagtacg
      121 ccgaaaaaag cacctcgcag taaattccct gctctgttag tggttgcgtt ggcgctggtt
      181 gcccttgttt tcgttatctg gcgcgtagac agtgcgccat caactaatga cgcttacgcg
      241 tcagcagata ccattgatgt ggtgccggaa gtcagcggtc gcattgtaga actggcggtc
      301 accgacaacc aggcggtgaa acagggcgat ttgctgttcc gcatcgaccc gcgcccgtac
      361 gaagccaacc tggcgaaagc cgaagcctcc ctcgcggcgc tggataagca aattatgctc
      421 acccagcgta gcgttgacgc ccaacagttt ggtgccgact cggttaatgc cacggtagaa
      481 aaagcccgtg ccgccgcgaa acaggccaca gatacattac gccgcaccga gccattactg
      541 aaagagggtt ttgtctcagc agaagacgtt gaccgtgcaa gaacggcgca gcgcgccgca
      601 gaagcggatc ttaatgccgt attgttacag gcgcagtcag ccgccagcgc cgtcagcggc
      661 gtcgatgcgc tggtggcgca acgtgcggcg gtcgaagcgg atattgccct gaccaaacta
      721 catctggaaa tgacgaccgt tcgcgcgcca tttgatggcc ggatcatttc cctcaaaacc
      781 tccgtcgggc aatttgcttc tgccatgcgc cctattttta ccctcatcga cactcgtcac
      841 tggtacgtga tcgccaactt ccgcgaaacc gatctgaaaa atattcgctc aggtacaccc
      901 gcaacgattc gcctgatgag tgacagcggc aaaaccttcg agggtaaagt ggattcgatt
      961 ggctacggcg tgctaccgga tgacggcggc ctggtgctgg gcggcctgcc aaaagtgtct
     1021 cgttctatta actgggtccg cgttgcccag cgttttccgg tcaaaatcat ggtcgataaa
     1081 cctgacccgg aaatgttccg catcggcgct tcggcagtcg ctaatcttga gccgcaataa
     1141 tgagcgcgct caactccctg ccattaccgg tggtcaggct gctggcgttc tttcatgaag
     1201 agttaagcga gcggcgaccc ggccgcgtgc cgcagatcgt gcaactctgg gtaggctgcc
     1261 tgctggtgat tctgatctcg atgacttttg agatcccttt tgtggcgtta tcgctggcag
     1321 tgctgtttta cggtattcag tcgaacgcgt tttacaccaa atttgtggcg atcttgtttg
     1381 tggttgccac ggtgctggag atcggcagcc tgtttttgat ctacaaatgg tcatacggcg
     1441 aaccgttgat ccgattgatc atcgccgggc cgatcctgat gggctgcatg tttttgatgc
     1501 gcacccatcg gctgggactg gtctttttcg ctgtcgccat ttacgggcaa accttccccg
     1561 ccatgctcga ctatccggaa gtagtcgtgc gcttaacgct gtggtgtatc gttgttggcc
     1621 tctatccaac cttgctgatg acgttaatcg gcgtgctgtg gtttcccaat cgtgccatta
     1681 cgcaaatgca tcaagcgctt aatgatcggc ttgatgatgc cattagccac ctgacggaca
     1741 gcctcgcacc gctacccgaa acgcggattg aaagagaggc gctggcgctg caaaaactga
     1801 atgtcttttg tctcgcggac gatgccaact ggcgaactca aagcgcatgg tggcaaagct
     1861 gcgtggcaac ggtaacctac atttactcga cgctgaatcg ctacgatccc acctcttttg
     1921 ctgattctca ggcaattatt gaatttcggc aaaaattagc ctcagaaatc aacaagctgc
     1981 agcatgccgt tgctgaaggt cagtgctggc aaagcgactg gcggctcagt gaaagtgaag
     2041 cggtggcggc acgggaatgt aacctggaga atatctgcca gacgttgtta caactgggcc
     2101 agatgaaccc gaatacgccg ccaacgcccg ccgccaaacc gccatcaatg gtcgccgatg
     2161 cttttaccaa tccagactat atacgctacg cggtaaaaac attgctcgcc tgtttgatct
     2221 gttacacctt ttacagcggc gtggactggg aaggcattca cacctgtatg ctgacctgcg
     2281 tgatcgtcgc taatccaaat gtcggttcgt cgtaccagaa gatggtgctg cgttttggcg
     2341 gggccttttg cggcgcgatt ctggcgctgt tattcacgct actggtcatg ccctggctgg
     2401 acaatattgt cgaattgctg tttgtgctgg caccgatttt cctgttgggc gcatggattg
     2461 ccaccagctc tgaacgctct tcttatatcg gcacacagat ggtggtcacc ttcgcgctcg
     2521 ccacgctcga aaacgttttt ggcccagtgt acgacctggt ggaaattcgc gatcgcgccc
     2581 tgggtatcat cattggtacc gtggtgtccg cgatgattta cacctttgtc tggcctgaaa
     2641 gtgaagcgcg cacgctgccg caaaaactgg ctggcgcgct gggtatgtta agtaaagtaa
     2701 tgcggatccc acgccagcag gaagtcacgg ctctgcgcac ttatctgcaa attcgtatcg
     2761 gtctgcatgc ggcgtttaat gcctgtgaag agatgtgcca acgcgtggcg ctggagcgtc
     2821 aactggacag cgaagaacgc gccttactga ttgaacgttc gcaaacggtt attcgtcagg
     2881 gccgcgatat ccttcacgcc tgggatgcca cctggaattc ggcgcaggcg ctggataacg
     2941 cactacagcc ggacagagca ggtcagtttg ccgacgccct ggagaaatac gctgccggtc
     3001 tggcaaccgc actcagccgt tctcctcaaa taacgcttga agagacaccc acctcgcagg
     3061 ccatcctgcc caccttatta aaacaggagc aacacgtctg ccagcttttc gcccgcttgc
     3121 cagactggac agccccggca ttaacgcccg ccacggaaca ggcacaagga gccacgcaat
     3181 gatcaatcgt caactttcac gtctgctgtt gtgcagcatt ctcggcagca cgacgctgat
     3241 ttccggctgt gccctggtac gtaaggattc tgcgcctcat caacagctca aaccggaaca
     3301 aatcaaactg gccgatgata ttcatcttgc cagctccggc tggccgcagg cgcagtggtg
     3361 gaaacaactc aatgacccgc agttggatgc cctgatccaa cggacgctaa gtggttcaca
     3421 caccctcgcc gaagcgaaac tgcgggaaga aaaagcgcaa tcgcaggccg atttgttaga
     3481 tgccggttca cagttgcagg tggcagcgtt agggatgctt aaccgccaac gtgtctcggc
     3541 gaacggcttt ttaagccctt atgcgatgga tgcgcccgca ctggggatgg acgggccgta
     3601 ctatacggaa gccacagtag gtttgtttgc cggactggat cttgatttgt ggggtgtgca
     3661 tcgctcagcg gttgccgccg ccattggcgc gcataatgcc gcgctggcag aaaccgcagc
     3721 agtagagcta tcgctgacca cgggcgtagc gcagctttat tacagtatgc aggccagcta
     3781 tcagatgctc gatctgttag aacaaactcg cgatgtgatt gattacgcgg tgaaagcgca
     3841 ccaaagtaaa gtggcgcacg gtctggaagc acaagtgcct ttccacggcg cgcgggcaca
     3901 gattctggcg gtcgataaac aaattgctgc cgtcaaaggg caaatcaccg aaacgagaga
     3961 atctctgcgc gcattgattg gcgcgggagc cagcgatatg ccggagatca aacctgtggc
     4021 attaccacga gtccagaccg gcattccggc gacactctct tatgagttgc tcgccagacg
     4081 cccggatctg caagccatgc gctggtatgt tcaggcgtca ttagatcagg tggattccgc
     4141 gcgggcgttg ttctatccga gctttgatat caaagcgttt ttcggccttg atgccatcca
     4201 cctggatacc ttattcaaaa aaaccagtcg ccagttcaac ttcatcccgg gtctgaaatt
     4261 gccgctgttt gacggtggac ggttgaatgc caatctcgaa ggcacgcgcg ccgccagcaa
     4321 catgatgatt gaacgttaca accagtcagt actgaacgcg gtgcgtgacg ttgccgtcaa
     4381 cggcacgcgt ctgcaaacgc tcaacgacga gcgagaaatg caggctgaac acgtggaagc
     4441 aacgcgcttc acccagcgcg ctgccgaggc cgcctatcag cgcggcttaa ccagccgctt
     4501 acaggccact gaagcccggt tgccagtact tgccgaagag atgtcattac tgatgctgga
     4561 cagccgccgg gtgatccaaa gcattcagtt gatgaaatcg ctgggcggcg ggtatcaggc
     4621 ggctcccgtc gtcgagaaaa aataaaatgt ctgccgcgtg atggctgtca cgcggtattt
     4681 cgtttcgtca cgtcaaaact gacgacagcc tgtttttcgt cagagttttg aataaatagt
     4741 gcccgtaata tcagggaatg accccaaata aaatgtggca taaaagatgc atactgtagt
     4801 cgagagcgcg tatgcgtgat ttgattaact ggagcgagac cgatgaaaaa agtcgtcacg
     4861 gtttgcccct attgcgcatc aggttgcaaa atcaacctgg tcgtcgataa cggcaaaatc
     4921 gtccgggcgg aggcagcgca ggggaaaacc aaccagggta ccctgtgtct gaagggttat
     4981 tatggctggg atttcattaa cgatacccag atcctgaccc cgcgcctgaa aacccccatg
     5041 atccgtcgcc agcgtggcgg caaactcgaa cctgtttcct gagatgaggc actgaattac
     5101 gttgccgagc gcctgagcgc catcaaagag aagtacggtc cggatgccat ccagacgacc
     5161 ggctcctcgc gtggtacggg taacgaaacc aactatgtaa tgcaaaaatt tgcgcgcgcc
     5221 gttattggta ccaataacgt tgactgctgc gctcgtgtct gacacggccc atcggttgca
     5281 ggtctgcacc aatcggtcgg taatggcgca atgagcaatg ctattaacga aattgataat
     5341 accgatttag tgttcgtttt cgggtacaac ccggcggatt cccacccaat cgtggcgaat
     5401 cacgtaatta acgctaaacg taacggggcg aaaattatcg tctgcgatcc gcgcaaaatt
     5461 gaaaccgcgc gcattgctga catgcacatt gcgctgaaaa acggctcgaa catcgcgctg
     5521 ttgaatgcga tgggccatgt cattattgaa gaaaatctgt acgacaaagc gttcgtcgct
     5581 tcccgtacag aaggctttga agagtatcgt aaaatcgttg aaggctacac gccggagtcg
     5641 gttgaagata tcaccggtgt cagcgccagt gagattcgtc aggcggcacg gatgtatgcc
     5701 caggcgaaaa gcgccgccat cctgtggggc atgggtgtaa cccagttcta ccagggcgtg
     5761 gaaaccgtgc gttctctgac cagcctcgcg atgctgacca gtaacctcgg taagccgcat
     5821 gcgggtgtaa acccggttcg tggtcagaac aacgtgcagg gtgcctgcga tatgggcgcg
     5881 ctgccggata cgtatccggg ataccagtac gtgaaagatc cggctaaccg cgaaaaattc
     5941 gccaaagcct ggggcgtgga aagcctgcct gctcataccg gctatcgcat cagcgagctg
     6001 ccgcaccgcg cagcgcatgg cgaagtgcgt gccgcgtaca ttatgggcga agatccgctg
     6061 caaactgatg cggagctgtc ggcagtacgt aaagcctttg aagatctgga actggtcatc
     6121 gttcaggaca tctttataac caaaaccgcg tcggcggcgg atgtcatttt gccgtcaacg
     6181 tcgtggggcg agcatgaagg cgtgtttact gcggctgacc gtggcttcca gcgtttcttc
     6241 aaagcagttg aaccgaaatg ggatctgaaa acggactggc aaatcatcag tgaaatcgcc
     6301 acccgtatgg gttatccgat gcactacaac aacacccagg agatctggga tgagttgcgt
     6361 catctgtgcc cggatttcta cggtgcgact tacgagaaaa tgggcgaact gggcttcatt
     6421 cagtggcctt gccgcgatac ttcagatgcc gatcagggga cttcttatct gtttaaagag
     6481 aagtttgata ccccgaacgg tctggcgcag ttcttcacct gcgactgggt agcgccaatc
     6541 gacaaactca ccgacgagta cccgatggta ctgtcaacgg tgcgtgaagt tggtcactac
     6601 tcttgccgtt cgatgaccgg taactgtgcg gcactggcgg cgctggctga tgaacctggc
     6661 tacgcacaaa tcaataccga agacgccaaa cgtctgggta ttgaagatga ggcattggtt
     6721 tgagtgcact cgcgtaaagg caaaattatc acccgtgcgc aggtcagcga tcgtccgaac
     6781 aaaggggcga tttacatgac ctaccagtgg tggattggtg cctgtaacga gctggttacc
     6841 gaaagcttaa gcccgattac gaaaacgccg gaatacaaat actgcgccgt gcgcgtcgag
     6901 ccgatctccg atcagcgcgc cgccgagcag tacgtgattg acgagtacaa caagttgaaa
     6961 actcgcctgc gcgaagcggc actggcgtaa tacagtcctt tctacagcct cctttcggag
     7021 gctgtttttt tatccattcg aactctttat actggttact ccctacccaa tcgtattatc
     7081 aaaatgaaaa aaattatcgc attgatgttg tttttgacat tctttgccca cgcgaacgac
     7141 tccgagcctg gcagccagta tttaaaggca gcagaggccg gggaccgacg tgcacaatat
     7201 tttcttgccg acagctggtt tagctccggc gatttgagca aagccgaata ttgggcacag
     7261 aaagccgccg acagcggtga tgctgatgcc tgcgcgctgt tggcgcagat caaaatcacc
     7321 aatccggtca gtctggacta tccacaagca aaagttcttg cagagaaagc ggcgcaagcg
     7381 ggcagtaaag aaggtgaagt aacgctggcg catattctgg taaatactca agcgggtaaa
     7441 ccggattatc caaaggcgat ttcgctgtta gaaaacgcct cggaagatct ggagaatgac
     7501 tccgccgtcg atgcccaaat gctgcttggt ttgatttacg ccaacggcgt gggcattaag
     7561 gccgacgatg acaaggcaac ctggtatttc aaacgcagct ctgcaatttc ccgaaccggt
     7621 tattccgagt actgggcggg aatgatgttc ttaaacggtg aggaaggttt tatcgagaag
     7681 aacaaacaaa aggcgctgca ctggttgaac ctgagctgta tggaagggtt tgataccggg
     7741 tgtgaagagt ttgaaaaatt aaccaacggt taaggtaggc ctgataagac gcggcaagcg
     7801 tcgcatcagg catccacaca ttgccggggg atatccccgg caatcttcaa ttattgatcc
     7861 gcagttttat caaatttgcc cagcacttca cgctcataag ccagcgcctt tttgcggtca
     7921 aatttgtgtt cccacttggc aatcaccagc accgccagcg cattacccac cacgttcagc
     7981 gcagtacgcg ccatgtcgag gatacggtca acaccagcaa taaacgccag accttccagc
     8041 ggaataccta cgctacccag cgttgccagc aacaccacaa acgacacgcc agccacgcca
     8101 gcaatccctt tcgaggtcac catcagcgtc agcaccagaa tgatttcctg ccagatggac
     8161 aggtcaatgc catacagctg agcgatgaaa atagcggcaa tactttgata cagcgtcgaa
     8221 ccatcaaggt taaaagagta accggtcggc accacgaagc tggtgatcga cgccggtgct
     8281 ccgtaggctt ccatcttctc aataattcgc ggcagtacgc tttcagagct ggcagtggag
     8341 tacgccagaa tcagctcatc tttcagaata cgaatcagga tccagacgct taacccgcac
     8401 agacgtgcca caattcccag cactaccagc gcgaagaaca gaatggcgaa atgcaccagc
     8461 agcaccagtt tcgccagtgg ccacagagac gagaaaccaa agttagccac cgtcaccgca
     8521 atcagcgcaa acacacccac cggtgcataa cgcatcacca tgtgagtcac tttaaacatg
     8581 gtttcagaga tggagcggaa cacggtcacc agcggttcac gatgcgtcgc gggcagggaa
     8641 gaaagcccca gaccaaacag caccgagaaa aagatgatcg gcagcatttc gcctttcgcc
     8701 atcgacgcca caatgttcgt cggcaccagc gacaaaatcg tgcccataat gccgtgggaa
     8761 ctgctttgta ccgcttccgt agtgctctga tatttcgaga tatcgacggt cgccagctgc
     8821 gacatatcca ccccggcacc gggctggaag acgttcgcca gagtgatccc caaaatgatg
     8881 gcgacggtgg tgatcacctc gaagtaaata atggttttcg cgccaatacg cccgagctgt
     8941 ttggcatcac caacacccgc gatacccacc accagcgtgg agatcacaat cggcacaaca
     9001 atcattttga tcagatggat gaagatatca cccgccggag agagcaaatt gacgaccagc
     9061 cagtcgcggc tgtcgctatg gtagtgcagg tagcttccca ggagaatgcc cagcaccata
     9121 gcaaacagaa tctgccaggc caggctgaat tttatatttt tcatcagaat aacttcctca
     9181 atggagcgcc gttccccgtt cagaaacgcg agaacgtgac atactgccag ggcatgaatt
     9241 gtgttgcgtt ggtttgtaga tttttttaac gcttatgatc agtatcattt gcgcggtatg
     9301 ttgcgcaacc ctttaacaac agagcttttc gctgcattaa tgcataataa cgcgagttta
     9361 tggcaggatt aatgaataac catttgttat ggaaagattt ttatttgttt aatctgcaat
     9421 aatactgatt ggtgattatt tttggcatat gttcattcga ttatttttta tgcagctcaa
     9481 tgacataatc ttcatcacca gatgattaat ccgatctccg ctgcaacagt tttgccatat
     9541 taatcgactc gaccagctgt gtccggttga tccggggtga gttgagatcc atcacttttt
     9601 gccatagttc gatggcttgc gcgtaattcg cctgcataaa cgcatcggaa gccagcagca
     9661 tcagagcggt gatttcgtta gagtccagcg cgagggcttt gtcgatcatt gcgcgagtct
     9721 gggcggtcat atgctggctg gcctggtaat aaagcaccgt cgccagcgcc gcatacagtt
     9781 cagcgttctc accacgtagt tgcaacgcct gacgatacgc cagcagcgaa ttgctgtaat
     9841 cgttttgcca cagatagtac tcgcccagta acgcccattg ttcgctgttt tgtggattag
     9901 cgcggatttt atcctgcaat gcctgaagct gcgcttccgg ggtttgctgg ctggcaaact
     9961 gatgtagcgg atcgcgctga cgctgatact ccgcacgtac cgcctgccat tttgggctta
    10021 acagataact gccgacacag aggaatacca tcaatatcgt gagtgttgtc agcattttca
    10081 ccggtatttg aggctgtttc atcgcttagc cctcactcgc cagaggatca gtgccatcag
    10141 caataacaac accactggca gcgcccacaa caccagcgtc tgacccgtta acggtgggtt
    10201 atagcgaaca aaatctccgt agcgttcggt catccagcca atgatttcga cttcgttctt
    10261 cccctccgac accatgctat aaacctgatg gcgcatactg accgccaccg gcgcattgga
    10321 ttcgagtaag ttttgattct ggcactgcgg acaacgtaac tggctggcaa tatttaacgc
    10381 ctgttgctgt tgttgcggat tggcgaattg ccaggtgtct acaacctgag cgcgcgcaaa
    10441 acaggtaaat aacagcagta gcgtgagaag ccctttattc atcgcgcttc ttcccccgcc
    10501 atccgcttaa caatgcgccc gcaatcatca acaatcctcc cccccagatc cagcgcacac
    10561 cgctttgtac atacaaacga aaagcgtaac gatccgctct ggttttttca cccatgaccg
    10621 cataccagtc atggatgccg ttccagcgaa ttgacggttc catcatttgc tgacggcgtg
    10681 cttcataaaa acgccgctcc ggcgttaatt caccaatgcg ttgctgatgg tcaaacagcg
    10741 ccactatcgc tttttcgctg gtgtaattgc ctctggcttg cagatcgagt cgctcaaaac
    10801 ggaaggtgta tcctgccagc gtcacctgct gacccggctg taaattcagg ctgatctcct
    10861 gacgactgac actggaaacc acgatccccg cggcaaataa cagcacgcca gcatgagcta
    10921 ccagcgccgg aagctgcacg cgtttgccag agacaaacgt cgccagcaca atcaccacca
    10981 gcatcaacag accaaacggt aacgtcgcgc ggttaaaata cggcgcgcca acggagaggc
    11041 gtccccagcc cagcaggccg taaatcatcg gataaagcgt tcccaccagc acgatcagta
    11101 gcactgcgca aaacagcaac agcgtagcga ggattaacat ttcccgcgat aaccccgaaa
    11161 aacgcaccgc cgggccaccg tcccgcgctc gccagccata cagagccaaa gacgcaaggc
    11221 taatcagtgc aaacaggctg aacaacggca cggcgcggac gttatccagt gcgaacgcat
    11281 gaaccgaaac cagaatgcca gaacggacaa ttaaggtgcc cagcagcgac agcatcagag
    11341 taactatcgc cagcaacagc gaccagtggc ggaaaatccc ccgctggcgt gtcagggaca
    11401 aactgtgcag cagcgcagtg gcagaaagcc agggtaataa agaggcgttt tccaccggat
    11461 cccagaacca ccagccgccc cagccgagtt cgcagtaggc ccaccaggaa ccgaggatga
    11521 tccccgccgt taatgcactc cagccaggta acgcccagcg ccagcaaatt cgggcgctgg
    11581 ccgcatcaaa ctcgccgcgc agtagactcg ccagcgccac gctcgccgct accatcaaac
    11641 cgccatagcc aaggtaaagc agcggtggat gaaagataag accgggatgt tgcagcatcg
    11701 gattgagatc gcggccttcg attgctggtg gaaatatccg cacgaaggga tcggaccaca
    11761 gcaccacaaa cagtagcagt gcggcgagca tgagagataa aacggctagc gtcagcggaa
    11821 atagcggatc gctttgctgc cgataatgcc aggcaaacag cgcgctccag gcggaaagca
    11881 gcagcaccca aagcagcagc gaaccttcat gaccgcccca caccgccgcc agtttgagtt
    11941 cccacgacag caggctgtag ctatgttggg cgacataaat gaccgagaaa tcgctgatga
    12001 gaaaacaata cgtcagtacg ccaaaggcga gcagcaggag cgcgaactgc gccagaatgc
    12061 cgatgcaagt gagtcgcatc atggcaggcc agcgcaaccg cactcccgca aaggccgtca
    12121 acggggtcaa cacgttgacc ccgagactta acaacaacgc cagaaaaccg acttcaggaa
    12181 ggaatacgtc cagaaaccac ctcttttctg gcttacgcca ccgtcaactg tccggcgtag
    12241 agaatgaaaa agcgcagcat caacacgccg accagactgg ccccacacgc cgccagcacg
    12301 gcaggaatgc cggaactgcg attgacccac ggtttgagca acattggcac aatcagcccc
    12361 agcccggcga caccaagcca gaaccaccac gtccagaaac cgccacctaa tgccgccacc
    12421 agcgcacgaa ctttaccgtc atcgcccagc gccagaccga caaaaaacgc caccagcagg
    12481 aagatttcac cccataccac ggggatttcc atccggtgaa caaagtgcgc ttccgtggaa
    12541 tgcgggttac tgcgttggcg tatcgccatg gcgatcagcg ccaccgccgc accggacgag
    12601 atgccggaga agaggagcag caccggcagg ctcgggttat tgaggaacgg atacgatttc
    12661 agcgccgaca gcagaaagcc ggtataagcc cccagcagca ccgccaacac cagcatcagc
    12721 gtttccagtc cgcgatgaac gggcgtcagc aggctcagaa ccttttgcac gatccccagc
    12781 ttcggcaacc agcgttgttg cagggcaagc aaatcatgtt caaagatttt cgccagccac
    12841 agcaccagca ccaccatgta gagctgaaac agcatcaccc ccatcgacat caccgaggta
    12901 aaactgtagt ggaacatcag cttccagaag gtccacggtc ttgtcaggtg gaagacgagg
    12961 atcaacagac cgaggatcac cgcgcccggc ccgacaatca gcgtggtgcg cagcaacgta
    13021 ctgtctgcac cgcccgcctg cgggtagaag cgacgtaaca gcacggccag cgtcaccaga
    13081 ccggcagaaa taccaatcaa aaacaggtag atggcaatcg gccagtccca caccagcgat
    13141 tcaaaatgaa atgcggaagt ctgcgtcatt ggctcacctc cccgtattta aacggtacgc
    13201 ggtacagctt cggtttggtt cccagcgcca gcttgtagcg gtaagtaggc ttctggcgca
    13261 gcagttgcga aatctcactg ttaggatcgt ccagattgcc aaacgtcagc gccttcgtcg
    13321 ggcaagcttc aacgcacgcg ggaagcttac cggcctgcaa attggtctta cgacagaaat
    13381 cgcatttgtc cgccgtcttc gtgaccggat ggataaagcg cacgcggtac ggacaggcgg
    13441 cgatgcagta ctgacaaccg acgcagagat caggattaac atcgacaatc ccgctagcgg
    13501 catcacgaaa agacgcaccg gtcgggcaga cgtcaacgca cggcgcatga tcgcagtgct
    13561 ggcaagactt acggaagaag cgatatttca catcaggaaa ttcgccctgc ggctcgctac
    13621 gaataatcgt caggcgcgag acgccttccg gcaccttgtt cacttcccga caagcatcca
    13681 tacaggcggt acagccgatg cataacgact cgtcatgcac catgccgtaa cgcaccccat
    13741 tgatattcaa cgttttcgcc accacgcgcc ctgcggtgcc gctgacggct gccagcacgc
    13801 cgacgccggt gagaaactgg cgacgagacc aggtcatggc tgctccttaa gcaacggaac
    13861 agacgccggg ttaaagttcg gattggtgcg ctgatcgctg tggcaatcga cgcaaatctt
    13921 aatccgtcct ttgtcactta acgtctgcat cgtatcttgc tgtggatgca gggaatggca
    13981 gctggcgcac gccactttgg ttacgtggac atcgtgcggc cagaacgctt tttgcaactg
    14041 ttccggcaga tgacaggaca tacagacgct gttctgctct ccgaccttat acatcggctc
    14101 gttaaagcgc atcacatctt tcaccccttc gcggtgttgc ggtgatgctt ggccgtgaca
    14161 gttggtgcag gtgaccggca atttgttatt cgggttgata acggaagcat gtttgccatg
    14221 cataccttcg gtatctggtt tatgacagtc cagacaggcg gcatccgggt tacgctgctg
    14281 ggtaacttcg tagcgatcac cagatgcctg caccgcaggg gtagcggtaa ttccgttcag
    14341 gctccacaac aggcccgacg ccagcacccc agcagttaat aacgaacgta atacgctcat
    14401 attcactcca ttgtgttttc cgccccttgc gaggcggaac ggggttattg gcttaacaga
    14461 ccgtttttac gtgcctgctc ttcccactgc gggatcaccg ttttgatgaa gtcctgcttc
    14521 tcggccttga tttgttccat gttcaggcca atggcctgct gggctttctc tttggtcgag
    14581 atatccggga tctggatttc atgggtgata cctttggtcg ccagcaggcg cgccagtttg
    14641 gtgcgcgcat ccgccgcttt atccatcgcc gtaccgagca tccgtagacc ttcttccggt
    14701 gcgtgcatat gaataccgtg ggaagcgatc gccagatccc aacgccactg ggcatgacgg
    14761 atatcgtcct gaatcggctt catttcggct tccgtcgcgc ctgcatccag cgccgctttc
    14821 gcttcgaagt gagcgtgaac cagttgatct tcaaccttga ttttcaggtc gttaatcgat
    14881 tgcttacgtt ccgcgaccac tttttgcaag gcagctttat cctgggtatg gcagttcgca
    14941 caagtctggg cgaagttatc aaacggatta ccaattttat ggtcggtgta gagtttgcct
    15001 tcggcgttct gcacttttgg catatggcag tcgatacagg tcacgttgtt tttaccgtga
    15061 atgcccgctg tccaggtttc atattccggg tgctgcgctt tcagcattgg cgttttcgac
    15121 agggagttag tccagtcaga gaaggcaatt ttgtcgtaat actgctccat attttcgact
    15181 ttcatgccgt catcccacgg gaatttaacc gctttgtttt tgccgtcgaa gtaatactcc
    15241 acatggcact gaccgcaaac catcgattgc tggtcgaaac gtccggcttt ctcaaaaggt
    15301 ttaccaatgg cttccatcgc gcgagccgca tacggacggg aaagggttaa ctccggtttg
    15361 cctttggcga actctggtga ggcggtgtta tggcagtcgg cacaacctaa gttgttgacg
    15421 atttccgggc cgccgcgcgc ccatttaccg tggaagtagc catcttcgcc gtctttctgg
    15481 atcagacgcg ccacatccgg gcttttacaa ctccagcacg ccatcggtag cggaccatct
    15541 tcagcgtttt tcggcgcacc ggtacgcagg gtttcacgca catcggtcac agcaaaggca
    15601 tgtccacgag gcttgttgta atcgcgcgag aagggatacc ccgcccacag gatcaccagc
    15661 cgtggatctt ccgccagggc gtcaacacgc tctgactgtt ccgaggtggc tttccaggag
    15721 agatattgat cgtgatgctg cggggcaaag gtttcattct tcgcttccac agttacaggt
    15781 tttgcgggag ctgccgtttg ttcagcgtga acagaagtga aaaagaaaaa aggaatcaat
    15841 aagctgaaga tacggcgtgc gtttattttt atccttgtca taggggcttc atccgaattg
    15901 cgccattgtt gcaatggcgg tttttattgt ttttcacgac agtaaccgca cctacactgt
    15961 catgacattg ctcaccccta tgtgtaacaa ataaccacac tgtgaatgtt gtctttaatc
    16021 aattgtaagt gcatgtaaaa taccacttta gagttagtca gtatcttcct ctttttcaac
    16081 agcatgcata actgcatgtt cctcaaagaa ttaatcaact tttgttggtg accttcaaaa
    16141 attaccctgc cgtttatttg caccattcta cttttgcgtg atctgtcgcc caaatactaa
    16201 acaaaactgc caatacccct acatttaacg cttatgccac atattattaa catcctacaa
    16261 ggagaacaaa agcatgagcc aaattcacaa acacaccatt cctgccaaca tcgcagaccg
    16321 ttgcctgata aaccctcagc agtacgaggc gatgtatcaa caatctatta acgtacctga
    16381 taccttctgg ggcgaacagg gaaaaattct cgactggatc aaaccgtacc agaaggtgaa
    16441 aaatacctcc tttgcccccg gtaatgtgtc cattaaatgg tacgaggacg gcacgctgaa
    16501 tctggcggca aactgccttg accgccatct gcaagaaaac ggcgatcgca ccgccatcat
    16561 ctgggaaggc gacgacgcca gccagagcaa acatatcagc tataaagagc tgcaccgcga
    16621 cgtctgccgc ttcgccaata ccctgctcga gctgggcatt aaaaaaggtg atgtggtggc
    16681 gatttatatg ccgatggtgc cggaagccgc ggttgcgatg ctggcctgcg cccgcattgg
    16741 cgcgatgcat tcggtgattt tcggcggctt ctcgccggaa gccgttgccg ggcgcattat
    16801 tgattccaac tcacgactgg tgatcacttc cgacgaaggc gtgcgcgccg ggcgtagtat
    16861 tccgctgaag aaaaacgttg atgacgcact aaaaaacccg aacgtcacca gcgtagagca
    16921 tgtggtggta ctgaagcgta ctggcggaaa catcgactgg caggaagggc gcgacctgtg
    16981 gtggcacgac ctggttgagc aagccagcga tcagcactag gcggaagaga tgaacgccga
    17041 agatccgctg tttattctct atacctccgg ttctaccgga aaaccaaaag gcgtactgca
    17101 cactaccggc ggttatctgg tgtacgcggc gctgaccttt aaatatgtct ttgattatca
    17161 tccgggtgat atctactggt gcaccgccga tgtgggctgg gttaccggac acagttattt
    17221 gctgtacggc ccgctggcct gcggcgcaac cacgctgatg tttgaaggcg taccgaactg
    17281 gccgacgcct gcccgtatgg cgcaggtggt ggacaagcat caggtcaata ttctctatac
    17341 cgcacccacg gcgatccgcg cgctgatggc ggaaggcgat aaagcgattg aaggcaccga
    17401 ccgttcgtcg ctacgcattc tcggttccgt gggcgagcca attaacccgg aagcgtggga
    17461 gtggtactgg aaaaaaatcg gcaacgagaa atgtccggtg gtcgatacct ggtggcagac
    17521 cgaaaccggc ggtttcatga tcaccccgct gcctggcgct accgagctga aagccggttc
    17581 ggcaacacgt ccgttcttcg gcgtgcaacc ggcgctggtc gataacgaag gtaacccgct
    17641 ggaaggggct accgaaggta gcctggtgat caccgactcc tggcctggtc aggcgcgtac
    17701 gctgtttggc gatcacgaac gttttgagca gacctatttt tccaccttca aaaatatgta
    17761 tttcagcggc gacggcgcgc gtcgcgatga agatggctat tactggatca ccgggcgtgt
    17821 ggacgacgtg ctgaacgtct ccggtcaccg tctggggacg gcagagatag agtcggcgct
    17881 ggtggcgcat ccgaagattg ccgaagccgc cgtagtaggt attccgcaca atattaaagg
    17941 tcaggcgatc tacgcctatg tcacgcttaa tcacggggag gaaccgtcac cagaactgta
    18001 cgcagaagtc cgcaactggg tgcgtaaaga gattggcccg ctggcgacgc cagacgtgct
    18061 gcactggacc gactccctgc ctaaaacccg ctccggcaaa attatgcgcc gtattctgcg
    18121 caaaattgcg gcgggcgata ccagcaacct gggcgatacc tcgacgcttg ccgatcctgg
    18181 cgtggtcgag aagctgcttg aagagaagca ggctatcgcg atgccatcgt aacccacaat
    18241 tgccggatgc gaatcggtaa cggtttgtag gcctgataag acgcgacagc gtcgcatcag
    18301 gcattgattg ccggatgcgg cgtaaacgcc ttatccggcc tacattcggc aagggttacc
    18361 cgagcgttaa ccttctccca taagggagcg ggaattaaaa caatccctac attacctctg
    18421 gagaatctgt gatgaatggt acgatttatc agcggataga agacaatgcg catttcaggg
    18481 agttagtcga aaaacggcaa cggtttgcca ccatcctgtc gattattatg ctggcagttt
    18541 atatcggctt tattttactg atcgccttcg cgcccggctg gctgggcacg ccgctgaatc
    18601 cgaacaccag cgtcacacgc ggtattccaa ttggtgttgg agtgattgtg atctcctttg
    18661 ttctcaccgg tatctacatc tggcgggcga acggcgaatt cgaccgtctt aataatgaag
    18721 tcctgcatga ggtacaagca tcatgaaaag agttctgacg gcgcttgccg ccacactccc
    18781 tttcgcagct aacgccgcgg atgctattag cggggccgta gagcgccagc caacgaactg
    18841 gcaggcgatt attatgttcc tgattttcgt cgtgtttacg ctcggcatta cctactgggc
    18901 atcaaaacgc gtacgttctc gtagcgacta ctacaccgca ggcggcaata tcactggctt
    18961 ccagaacggg ctggcgattg ccggggacta tatgtccgcc gcctcattct tggggatctc
    19021 cgcgctggtg tttacctccg gctatgacgg gctgatctac tcgttgggct tcctggtggg
    19081 ctggccaatc attctgtttc tgattgccga acgtctgcgt aacctgggac gctacacctc
    19141 tgccgatgtg gcctcttatc gtctgaaaca agggccgatt cgtattcttt cggcctgtgg
    19201 ttctctggta gtggtggcgc tttaccttat cgctcagatg gtaggcgcag gtaaactgat
    19261 cgagctgctg tttggcctta actatcacat tgcggtggtg ctggtcggcg tgctgatgat
    19321 gatgtacgtc ctgttcggcg gcatgctggc gaccacctgg gtacaaatta tcaaagctgt
    19381 gctgttgctg ttcggtgcca gctttatggc ctttatggtg atgaaacacg tcggctttag
    19441 cttcaacaat ctgttcagcg aagcgatggc ggtacacccg aaaggtgtcg acatcatgaa
    19501 accgggcgga ctggtgaaag atccgatctc cgcgctctct ctggggctgg gactgatgtt
    19561 tggtacggcg ggcttgccgc acattctgat gcgcttcttt acagtcagcg atgcccgcga
    19621 agcacgtaag agcgtgttct acgccaccgg atttatgggc tacttctata ttctgacctt
    19681 tattatcggc ttcggcgcga tcatgcttgt tggtgcgaat ccggaatata aagacgcggc
    19741 gggccatctg attggtggta acaacatggc ggccgttcac ctggcgaatg cagtgggcgg
    19801 caacctattc ctcggtttta tttcagcggt tgctttcgcc actatcctcg cggtggttgc
    19861 ggatctgacg ctggcgggcg catcggcggt ttcgcatgac ttgtacgcta acgtcttcaa
    19921 aaaaggcgcg accgaacgtg aagagctgcg ggtatcaaaa atcaccgtac tgatcctcgg
    19981 cgtgattgcg attatcctcg gcgtgctgtt tgagaatcag aacatcgcct ttatggtagg
    20041 ccttgcgttt gccatcgcgg cgagctgtaa cttcccgatc attctgcttt ccatgtactg
    20101 gtcgaaactg actacccgtg gcgcgatgct gggcggctgg ctggggctga ttaccgcagt
    20161 ggtgctgatg atcctcggcc cgactatttg ggtacagatc ctcggtcacg aaaaagccat
    20221 cttcccgtat gaatacccgg cgctgttctc tatcagcgtg gcattcctcg gcatctggtt
    20281 cttctcggca accgataact cagcggaagg ggcgcgcgag cgtgaactgt tccgcgcgca
    20341 gtttatccgc tcccagaccg gctttggcgt tgagcaaggc cgcgcacatt aatctctccc
    20401 cttccccggt cgcctgaccg gggaatacgc ttcctctcca acatgcatca ccttttccca
    20461 taatattaaa caaataaact cattaaaaaa tgagcgattt ttgacagtcg tagaaaatga
    20521 taatgcagag aatatgcctt ttctttcttg ttaattataa ggatatttta tgcgctataa
    20581 tggtttaaat aatatgtttt tccctctttg ccagattaac gataaccact ctatcacaag
    20641 ttcatcacat acaaagaaaa caaaatcaga taattacagc aaacatcata aaaacacgtt
    20701 aattgacaat aaagccctct ctcttttcaa aatagatgat catgaaaaag tgataggctt
    20761 gattcagaaa atgaaaagaa tttatgatag tttaccatca ggaaaaatca cgaaagaaac
    20821 ggacaggaaa atacataaat attttataga tatagcgtta tatgcaaata ataaatgtga
    20881 cgatagaatt acgagaagag tttaccttag taaagaaaag gatgtatcca ttaaggtggt
    20941 atattttata aataatgtcg ccgtccataa taatactatc gaaattccac agatagtaaa
    21001 tggtggttac gatttttcac accttagcct gaagggtatc gtgattaaag atgaagattt
    21061 atccaattcg aattttgcag gttgcagact acaaaacgct attttccagg actgtaatat
    21121 gtataaaacg aatttttatt acgccataat ggaaaaaata ctttttgata attgtattct
    21181 cgatgactca aatttcgctc agataaaaat ggccgacgga actctaaatg catgctccgc
    21241 tatgcatgtt caattctaca atgcagcaat gaatagagcc aatattaaaa ataccttcct
    21301 tgactattca aatttttata tggcgtacat ggctgaggta aatctttata aagtaatagc
    21361 gccatatgtt aatttattta aagccgacct tagtttctct aaactcgatt taattaactt
    21421 tgaacatgct gatctgtctc gcgtcaatct gaacaaagca atcctccaga atataaactt
    21481 aattgatagc aaactctttt gtacgtggct gacaaataca ttcctcgaaa tggttatatg
    21541 taccggctct aatatggcta atgttaattt taataatgcc aatttaagca actgccattt
    21601 caactgttct attttaacaa aagcctgtat gtttaatacc cgtctctatc gggttaattt
    21661 cgatgaggct agcgtccagg gaatgggcat ttccattctc cgtggggagg aaaatatccc
    21721 cattgatagt gataccctgg taacactaca gaaattcttt gaagaagatt gtacctctca
    21781 tactggcatg tcacaaactg aggataatat taatgcagtc gctatgaaga ttactgcaga
    21841 tattatgcaa cacgcagatt gaggttaagt cgaagaacag ccaatgaatt gactttacaa
    21901 gagagataac ttatttgttt tagaatcatg aatgttcgcc cgagctaacc tgttaaaggt
    21961 gcaaaagatt gcaccttttt cagattacct cgggcctggt tacgtcgctt tcggcttact
    22021 gattttcctc aattagcaac gcttcaagca aatcgagatc gtgcagtaat ttttgcagcg
    22081 tttcgttgct gatctcccgc gtggcgcgca ggtggtacag ttcagcacgt tcagaacgca
    22141 atgccgccag acggaagcga cgctcaaggt tctcttcctg cacggaactt tcaacgtcgt
    22201 tacgtccatc ggcgcgacga cgcaggttac caatgacgcg agaactgacc tccgtaagca
    22261 actggttatc gatattctct tcggtatccg ccgccagacg ctcctccatt ttctggatcg
    22321 ccacaatcgc cacttctgcc gttgccgctc gcgcaatacg ttcttctttc aattgttgcg
    22381 aatggtccgc gacttcaatg tgttgtagca gaataggcaa catcaccacg ccgacaaaca
    22441 gcgagaagag aatgacgcca gccgccagga acaccagctc atagcgcgcc gggaagacgt
    22501 taccatccgg caagagcagc gggattgaga gcacaccggc cagagtgatc gccccacgca
    22561 ccccagcgaa agacgcgatc aggatttctc gtgtggtcca cgaaccaaac tccatcggct
    22621 tcttcttcag gaagcggttg ctgaactttt tcatcgtcca cagccagccg aaacggacca
    22681 gcatcagcgc cgcatatatc agaataatat cggtaaacag catccagatt tcgacgttag
    22741 ggtcgatttc tgccgccatc agcgacgtct ccagaatacc cggcagttgc agacctaaca
    22801 gcaggaacac catgccgtta aagacaaatt ccagcatcgc ccaggtgctg tttgcgcgca
    22861 ggcgcattgc cagtggcgca cggcgcatca caccggagcg ggtgatggtc atcccggcgg
    22921 caaccgcagc gaggatgccc gaaacgccaa tatgttcggc aatcagataa gaagcgaatg
    22981 gcagcaagaa cagcagaacg atctgcgtcg cgggttcatc accgccccag cggctgagga
    23041 atcgcagcga gcgaccatac agccagctca ccacaaaacc ggcaagaata ccgccaatgg
    23101 ctactttcat aaattcgacc gtcgcaccgc cgacagtgaa gatcatcgtc cccatcgcca
    23161 ctgccacggc aaacttcaac gacactaggc cggaagcgtc attcatcaac gcttcgccct
    23221 gcaaaatgcc catgattttt ttcgggatgc gcccttcgcc aacaatcccg gagagcgcca
    23281 cagcatcggt cggagaaagt accgccgcca gcgcaaaggc ggggatcagc ggaatgcccg
    23341 gcaccaccca gtaaataagg aagccaatgc cgaccacggt gaccaccacc agcgccagcg
    23401 cgaggccgaa aatctctcga ccatgttcga gaaattcacg ggtcggcgtt ttccagccat
    23461 cagcgaacag caacggcggg ataaacaaga ctaaaaagag ttcaggatca aactccacat
    23521 gcaaaccaaa cgtcggccac gccagtagcg caccgatggc gatttgcata agcggaagcg
    23581 ggatctgaaa gggcatgaca cgagtgacca ccccggacag cgagaccacg agggtcatta
    23641 tcagtatggt gaagaagatt tccatgcgtt ccctgtttgc gatgtttttt atgatctgag
    23701 aaagacgtta tgcctgaatt ctcatttttt cagaatatcg caaaagctca cacaatgtgc
    23761 ggagaaaata aaaacgggca gccattgcca cccgttttca gaatccgatg aaagattaga
    23821 tagcccagcc acccgcatag aaggtcacca gcgccacggc gataacgacc gtaccgatgt
    23881 tcaacttgcg ccattcaccg gaaaccagac gaccaatcac cagagtcgcg aagccgatca
    23941 tgatgcctgt tacgatgtta caggtcagca cgatgaatac cgccgtaacc agacccgcca
    24001 tcgcatcaac aaaatcagca aagtcgattt tcgccacgtt gctcagcatc agcaggccaa
    24061 cgtacatcag cgccggagcc gttgcatacc ccggaacgag gtaagaaagc ggagagagga
    24121 acagaatcag caggaacagc acgccaacgg tgatagccgt caacccggtt ttaccgcccg
    24181 ccgccgtacc cgccgcagac tcgatgtata ccgctgccgg agctgcaccc accaggccag
    24241 agaaaacgct gctcatggag tcagtggtca gtgctttccc gccgtcgatg atctgcccat
    24301 ctttatccag caggttcgcc tggccggcga cagcgcggat agttccggtg gcatcaaata
    24361 ctgccgtcat caccagcgcc agaacgcttg gcaggactac aggattcagc gcgcccataa
    24421 tgtccaggct gccaatcagt gaattgccgt tttcatcgct caatgaaggc atggcgaaaa
    24481 cgccggagaa atggacgtta ggatcgaaga tcaaaccgac aattgagata ccgataatgg
    24541 tcagcagaat gccaccaggg actttcagtt tttccaagcc gatgatcacc gccagacctg
    24601 ccagtgacat aatcaccggg aaggtcgcga aatcacccag cgcaacgggc agaccatcaa
    24661 gcgggttttt aatcaccaga ccgacaccat tagcggcaat gagcagcagg aacagaccga
    24721 taccgatccc cgtgccgtgc gccacaccgt gcggcaagtt gcgcaaaatc cagctacgga
    24781 tacccgtggc agaaattacc gtaaacagca cacccatcag gaacacggca cccagcgcga
    24841 caggtacgct aatatgttgc cccagcacca ggctgaatgc ggtaaacgcc gtcagggaga
    24901 tggcgcaacc aatcgccaac ggcagattag cccacagacc catcacgata gaaccgagtc
    24961 cggcaaccag acaggttgca acgaaaactg ccgcaggcgg gaagcctgct ttacccaaca
    25021 tacctggaac gacgatgacc gagtagacca tcgccagaaa cgttgttaac ccggcaacca
    25081 cttcctgacg gacagtgctt ccacgttgtg aaattttaaa ccaggcgtcg agtgaaccgc
    25141 cggtacgcgc tgatggcgta gacataagaa aacatcccct gagagtttaa ttttcgtcaa
    25201 gatgcgtggc ggacaatccc ctgccagcga aacgttatct tcctgctcca ggtttgcggc
    25261 aatttggtgc gtcgcaaaaa agcaaacgtt tagctccgcg catacaaaac gggtggtaaa
    25321 aaaaagcaaa cgattatccg gcgatcatag cggctttttc aaccgaagtt tgctgctttt
    25381 ttctctttta ttgtcttgac gatgaaaaac caggcaattg atgcgcaaac gttatcgtcg
    25441 atgtgcaaga ttgcagcgtt atcgagccgt ataccgttct ggattgcgat aaaaaatcgt
    25501 ctgagatgac aaaaatattg cggctaaaaa gtatactttt acactggcga agaaacgtcg
    25561 gtgacataca attaaagcat caacaccaac cggaacctcc accacgtgct cgaatgaggt
    25621 gtgttgacgt cgggggaaac cctcctgtgt tccagcggga tagagagaaa gacaaagacc
    25681 ggaaaacaaa ctaaagcgcc cttgtggcgc tttagttttg tgtttgcgga taattccaaa
    25741 agagctcgat cagtcccctc gccccttcgg ggagagggtt agggtgaggg gaacaggcag
    25801 caccagtgca aatatttacc ctcaccccgg ccccatagtt aacgatgagt cagcttgtcc
    25861 ttgtggcgct ttagttttgt tcatcttcca gcaagcgtgc gccggtacct tcttctccta
    25921 agcggtcgcc cgggttacgc aacgggcaat cactgcgcga aaggcagcca caaccaatac
    25981 atccgtccag ttcgtcacgc agcgccacta aggtatgaat gcgccgatcc aactcttctc
    26041 gccattggga cgaaagctgt ttccactctt tcgcacttaa cgtatgccct tctggcaaca
    26101 cgccaaacgc ttcaccaatg gtcgccagcg gaatgccaat acgctgagcg attttgataa
    26161 ttgcgacata tcgcaacaca tcacgtttat atcgccgctg attgccgctg ttacggatac
    26221 tagtaatcaa ccctttgctt tcatagaaat gcagcgccga taccgccaca ccgctgcgtt
    26281 tcgccacttc gccaggggtt agcagcgctt taatgcgggg taatttcttt tccataaatc
    26341 gctttacctc aagttaactt gaggaattat actccccaac agatgaatta acgaactgaa
    26401 cactgaaaag aggcagattt atgtcccatc agaaaattat tcaggatctt atcgcatgga
    26461 ttgacgtgca tattgaccag ccgcttaaca ttgatgtcgt cgcaaaaaaa tcaggctatt
    26521 caaagtggta cttgcaacgg atgttccgca cggtgacgca tcagacgctt ggcgattaca
    26581 ttcgccaacg ccgcctgtta ctggccgccg ttgagttgcg caccaccgag cgtccgattt
    26641 ttgatatcgc aatggacctg ggttatgtct cgcagcagac cttctcccgc gttttccgtc
    26701 ggcagtttga tcgcactccc agcgattatc gccaccgcct gtagttttat tgcccgcgcg
    26761 ttaactcccg cgcgggtaat tgctccatcc attgcataaa cacctgcggc ggcatcgcct
    26821 tcgcaaaaaa ccatccctgg caatagcgca cgccgcgctt gcgcagccag ttaacctgct
    26881 cctcagtttc gacgccttca gcgatcgttt ttaaccccag gctgtgcgcc agctcgatga
    26941 tgtgttccgc aatcaaatga ctggttttgt gggtggtcag cgtttcaaca aacgatttat
    27001 cgattttcaa aatatcgaca ttcaatgatt taaggttatg caagttagag tagccaatac
    27061 caaaatcatc aattgccact tcgtaaccag cctggcggaa agccagaatt aatcggcgtc
    27121 attttttcga catcaagaaa tgcatgctca gtcacttcaa atttaatttg ctgcggacgc
    27181 accgcgtatt gctctgtttt ctgattgatt cgcgctatca accgtgacgt atgaaaatcg
    27241 gaggccgaca ggttaataga aacatagcga tcggcatgtg ttgccaggta cgcgcccaga
    27301 tcgcggaaga cattatcaat aacataatca gttatctgtt ctatcatccc ctccttttct
    27361 gccagcggaa taaactctgc cggattcatt atttgcccct gctcaccagg ccaacgtaac
    27421 aacgcttcag cgccgataca tttttctgtt ttgatatcga ttattggctg gtaataaaga
    27481 caaagttgat gtttttcgag ggcgcgttgc aatttacgtt tgggagataa atagttttgt
    27541 cgaatacgta gccagagtaa tagcagaaca agactcccca aaataccggc gggcaacgca
    27601 aaaataagat gattataaaa atgggttata agacgttgat atgaagtcgc aacaatggct
    27661 gcaattgggc gttttgtcga ataaattgtc gcatataaat agccatttct ttgtacggtt
    27721 aaatccttca aatgaatcag cggagaaaac gttgctgccg aggcctcttt gcttaacgag
    27781 aaaaaggttt tcatcaccgt atcatacaca ccccattgca atgtcgggtc atcagacatc
    27841 acttcactcc agaagagagg gttgataacc gccacataat ttccccgctg catataggtc
    27901 attttatagc cagagaaaaa aggcgtatcg cggtaataat agatagaaac gttaggttca
    27961 cgcttataat cggccggtgc aatcgtatag ccgtttacag gcgctatcag cgatgagcat
    28021 aaaaaatggt tatcacgaat tcattaatat aaagatagcc acgaataata ttcaacattc
    28081 gcttttgatg ggctggagtg caaacttgcc cctgatagcg ttcagctgca tcgctcacta
    28141 aatctgcctg gcgaatgacc agctcggatt tatccagtgc cagttgagca aaggttcgga
    28201 gctgattatt cacttctgat tttgcccaaa ggaaggcaat ccatagcgaa agaatgatgg
    28261 gaaagagaac taaaaagata atgcccggca acgccagtaa ttggtgtcgt gcacgatgac
    28321 tcattgctat caatttcctg cctgtttata cccggccatt gcaaggtgaa accctggcta
    28381 ccgacgtctt tagataaatc ttacggtata aatagcactt ttgcctgatt tgaaattgat
    28441 acctttttaa tcgtcacctg gatgaaaata attcattgat attcaaaatt aattttctac
    28501 aggaatacag aggcggcggg aagatatgcc cttaatgcca ctgaaaaacc acataatttg
    28561 tacagcaata aaagtcacgg cctaatatgg ctaacaaaca atcaattgtg atatagttca
    28621 caaaattaat gaaacaaaca gagtgtttca tttttgtgtt ctgcaagccc cgcgatagcc
    28681 agagtgtacc acgcctcccg tgaacaacgc cgcgctgtcc agggcatcgg ctcttttcag
    28741 aggaattgtt tgatggctac cctcaccact ggcgtggttc ttcttcgctg gcaacttctt
    28801 agtgccgtaa tgatgtttct ggccagcaca ctcaacatcc gttttcgtcg gtctgattat
    28861 gtcgggcttg cggtgatcag cagcggtctg ggcgttgttt ccgcctgttg gttcgcaatg
    28921 gggttgcttg gcatcacaat ggcggacatc accgctatct ggcacaacat cgagtcggtg
    28981 atgatagaag agatgaatca gacaccgcca caatggccaa tgattttgac ctgatacgta
    29041 gaa
//
LOCUS       DAEBXN010000045        28914 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.045, whole genome shotgun sequence.
ACCESSION   DAEBXN010000045 DAEBXN010000000
VERSION     DAEBXN010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28914
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.045"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="KJL99_002033"
     CDS             complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="KJL99_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HBD4436765.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQSYQHDPHFERAFTHIDAALERIRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPRNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQI"
     gene            complement(2138..2584)
                     /locus_tag="KJL99_002034"
     CDS             complement(2138..2584)
                     /locus_tag="KJL99_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HBD4436766.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            2707..4761
                     /gene="helD"
                     /locus_tag="KJL99_002035"
     CDS             2707..4761
                     /gene="helD"
                     /locus_tag="KJL99_002035"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HBD4436767.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSGGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="KJL99_002036"
     CDS             complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="KJL99_002036"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HBD4436768.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(5347..6009)
                     /locus_tag="KJL99_002037"
     CDS             complement(5347..6009)
                     /locus_tag="KJL99_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HBD4436769.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            6182..6595
                     /locus_tag="KJL99_002038"
     CDS             6182..6595
                     /locus_tag="KJL99_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HBD4436770.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="KJL99_002039"
     CDS             complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="KJL99_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HBD4436771.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="KJL99_002040"
     CDS             complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="KJL99_002040"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HBD4436772.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            8300..8578
                     /gene="yccX"
                     /locus_tag="KJL99_002041"
     CDS             8300..8578
                     /gene="yccX"
                     /locus_tag="KJL99_002041"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HBD4436773.1"
                     /translation="MSKICIIAWIYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="KJL99_002042"
     CDS             complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="KJL99_002042"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HBD4436774.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="KJL99_002043"
     CDS             complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="KJL99_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HBD4436775.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMIANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(9861..9948)
                     /locus_tag="KJL99_002044"
     tRNA            complement(9861..9948)
                     /locus_tag="KJL99_002044"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9912..9914),aa:Ser,seq:tga)
     gene            10374..11492
                     /gene="hyaA"
                     /locus_tag="KJL99_002045"
     CDS             10374..11492
                     /gene="hyaA"
                     /locus_tag="KJL99_002045"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HBD4436776.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            11489..13282
                     /gene="hyaB"
                     /locus_tag="KJL99_002046"
     CDS             11489..13282
                     /gene="hyaB"
                     /locus_tag="KJL99_002046"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HBD4436777.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            13301..14008
                     /gene="hyaC"
                     /locus_tag="KJL99_002047"
     CDS             13301..14008
                     /gene="hyaC"
                     /locus_tag="KJL99_002047"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HBD4436778.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            14005..14592
                     /locus_tag="KJL99_002048"
     CDS             14005..14592
                     /locus_tag="KJL99_002048"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HBD4436779.1"
                     /translation="MSEQHVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            14589..14987
                     /gene="hyaE"
                     /locus_tag="KJL99_002049"
     CDS             14589..14987
                     /gene="hyaE"
                     /locus_tag="KJL99_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HBD4436780.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGAFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            14984..15841
                     /locus_tag="KJL99_002050"
     CDS             14984..15841
                     /locus_tag="KJL99_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HBD4436781.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            15975..17519
                     /gene="appC"
                     /locus_tag="KJL99_002051"
     CDS             15975..17519
                     /gene="appC"
                     /locus_tag="KJL99_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HBD4436782.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            17531..18667
                     /gene="appB"
                     /locus_tag="KJL99_002052"
     CDS             17531..18667
                     /gene="appB"
                     /locus_tag="KJL99_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HBD4436783.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSHPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNKNELY"
     gene            18679..18771
                     /gene="cbdX"
                     /locus_tag="KJL99_002053"
     CDS             18679..18771
                     /gene="cbdX"
                     /locus_tag="KJL99_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HBD4436784.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            18851..20149
                     /gene="appA"
                     /locus_tag="KJL99_002054"
     CDS             18851..20149
                     /gene="appA"
                     /locus_tag="KJL99_002054"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HBD4436785.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESMVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     AQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILCRAGGSIADFTGHRQTVFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(20264..22430)
                     /gene="etk"
                     /locus_tag="KJL99_002055"
                     /pseudo
     CDS             complement(20264..22430)
                     /gene="etk"
                     /locus_tag="KJL99_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
     gene            complement(22450..22896)
                     /gene="etp"
                     /locus_tag="KJL99_002056"
                     /gene_synonym="yccY"
     CDS             complement(22450..22896)
                     /gene="etp"
                     /locus_tag="KJL99_002056"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HBD4436786.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYSMLERASQEWAKRLSR"
     gene            complement(22884..24023)
                     /locus_tag="KJL99_002057"
     CDS             complement(22884..24023)
                     /locus_tag="KJL99_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HBD4436787.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(24069..26165)
                     /locus_tag="KJL99_002058"
     CDS             complement(24069..26165)
                     /locus_tag="KJL99_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HBD4436788.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAWQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGKSEQLQQQRVEAEDLSAFGRCYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDDGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKLFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLDPMTAEREVPVGQ"
     gene            complement(26165..26911)
                     /locus_tag="KJL99_002059"
     CDS             complement(26165..26911)
                     /locus_tag="KJL99_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HBD4436789.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPSEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(26908..27552)
                     /locus_tag="KJL99_002060"
     CDS             complement(26908..27552)
                     /locus_tag="KJL99_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HBD4436790.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVAAQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNPGRAPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGHNV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(27659..27964)
                     /locus_tag="KJL99_002061"
     CDS             complement(27659..27964)
                     /locus_tag="KJL99_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4436791.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTSGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(28406..28618)
                     /gene="cspH"
                     /locus_tag="KJL99_002062"
     CDS             complement(28406..28618)
                     /gene="cspH"
                     /locus_tag="KJL99_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HBD4436792.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
BASE COUNT         7003 a         7322 c         7600 g         6989 t
ORIGIN      
        1 taatctggcg ttgcagacga ccaatctctg gcagtaatgc aatcaataac ccaacttgtt
       61 gtacgaccag aggttctttg ctatctgccc gtggttcaag ttgctgcatc cgctgtttca
      121 ggccagctaa tgcctgattg acgcgttctt catcagcagg ttgatgatgt aacgcgtcat
      181 caacatagca aactgcgtca tcaagaaacg ccagaatttc aggattagtt aactgctccc
      241 ggtgagcacc gagggctgag atatagctgg taaacgtatg gttaaggcac agcaagcgaa
      301 acgcggcttc gcgaatttgc ggggtaacgt tcggttcgct ggacatattt gataccaccg
      361 acgccagttc agcatcacgg ttgtgtgcat cgcggcgggc aatacgatac gccagacggt
      421 tatcacgccc ctgatggtat tgctccagta tggcatcgag atagcgacag ttggcctctg
      481 tggcgcgttc gagcattcgc ggcagattgc gaaacttcca gtcaggccag atgtagctca
      541 ctgccgccca cgcaatggca caaccaatca gcgtatcgat tacgcgaggt aacgctactt
      601 caaaaccttc acccagtaag ttaaaacaca gtagcaccaa aagtgtgatg aacatcgttg
      661 catgagcgta ttgcacgtta cggaaggcaa aaaagagcac gccggtaata accagcagca
      721 ccagctgccc ttccagtgat ggcacaaacc acagcacagg aatgccaatc gcgataccta
      781 ccagcgtacc aataatcctt aacttcaggc ggtggcgcgt ggcgttatag tttggctggc
      841 agacaaacaa acttgtcagc aagatccaat acccgtgatg cattccggtt atttgaatga
      901 tggcgtagcc gaagcacaac accagcgaca ttcttaccgc atgacggaag agggcggatt
      961 ccggcgtgaa gtgacggcta agacgcagcc agatatcact caatccgtgc gggctgtcat
     1021 cagcgagctc attttcgtca ttattacggg gtagtgcctg ggcctgttct gattcaattg
     1081 ttgccagttg ggcatcaatg gcgcgtaaat tgttcagcaa aaatcccagt gttttgagta
     1141 aatcggcggg tgcgccgtta tcgcgtatcc gctccagcgc agcatcaata tgcgtaaaag
     1201 cgcgctcaaa atgcggatca tgttgataag actgacgcaa caaaatacag cgtgacagtt
     1261 gctggcacgc ctggccctgc atcgacatca gccgctgaaa acggaacagc acgtcgctgt
     1321 ggcgaaaatg ttcacgcaat gtttgatact gaatatgaga agagctggca cgctcgtgaa
     1381 tatcctgtgc gacaaagtaa taatgcagcg tgcgacgcgt tccccgttga ccacgatcgc
     1441 cacgtaagcg ggtcagcagc gagagtttcg tctgattcaa tgtcgccatc agctgaccgt
     1501 tggcgagagc caaatcgtac agcggtgcct ggctttcatc ttcaatatca ggatcaaaca
     1561 tgcgcgactt gagctcaaga taacgcgcca gttgttcata gcaacgcgcc aggttgtcct
     1621 gcagcgggcg gaccgggaac agcagatgac caataagtgt caggacgttg taccagacgg
     1681 caccggccag cagatacatc ggctgctgat accagtgctc atacagtgat gttcccaaca
     1741 tagtgtaaat ggcgatcagc aatgcaccga aggcaattgt tgcatagcgt tgacccagac
     1801 cgccgagcaa aatgaagccg ctggtagaga gcgttaagcc aatcgcaaat agccagggcc
     1861 agggaaacag caattctact gaggccgagg cgataaaaaa gcagaacagc gtaatgatga
     1921 ggttacgcaa acgtcccgcc agtcggtcat cgagatcggt cagcgccgct gccaccatcc
     1981 ccagcgttag cggaatcgtc agttttacat cacccagcca ccacggaaac gctgtggttc
     2041 cacaaagcgc aataaaaata cgcgcgtaat acagccaggc gctgttccag gtatagcgtt
     2101 tgagcaaagg acttagcata aaggccatat tagtggatta ttcaaaacga cgacgtgcat
     2161 tggcttcacg cgcagcttgc gctgtttcta ccgataccac gcgacgacca accggccata
     2221 gtgcaatagc ggcaatttta aagttcgcaa tgccgacagg aatgccaatg attgaaatac
     2281 attgtgcgat gcccgttgca atgtgcatca ggcataacca ccagccaaag aaaatcagcc
     2341 agaaaatatt caataccgta ccgccagtat tcagcaacac atttttgcca gccgggttca
     2401 gttcatcgac atgaatagct tcattgccat aaggcaccag agacagttta gtgatctccc
     2461 agcaggatcg tgtcagcggt aaggtaaaaa tcagcacaat actgaccaga gtcgccaaca
     2521 gccagcccag agtggtggca aatccgccaa gcacaaagtt cagaatgttc aaaacggtac
     2581 gcataaaacc tcgctttact gtggttttca gtaatgggcg gcaattgtaa cgtttttttg
     2641 ggctggagca cgttttctct gacggttaca ctgataagaa ataatttcgt gtggatctac
     2701 agagtcatgg aactgaaagc gacaacgctt ggaaaacgtc tggcacagca cccttacgat
     2761 cgggcggtga tcctcaatgc cgggattaaa gtctccggcg atcgccacga ataccttatt
     2821 cctttcaatc aattactggc gattcactgt aagcgcggtc tggtatgggg cgagctggaa
     2881 tttgtactgc cggacgaaaa agtggtgcgt ctgcacggca cagaatgggg cgagacgcag
     2941 cgtttttacc atcatcttga tgctcactgg cggcggtgga gtggcgagat gagcgaaatt
     3001 gcgtctggtg ttttacgcca gcaactggat ttgattgcca cgcgcactgg agaaaataaa
     3061 tggctgacgc gtgagcaaac ctctggcgtt cagcaacaaa tccgccaggc tttgtcggcg
     3121 ttgccgttgc cggttaaccg actggaagaa ttcgataact gccgtgaggc gtggcgtaaa
     3181 tgtcaggctt ggttgaaaga tattgaaagc gctcggttgc agcataacca ggcgtatacc
     3241 gaagccatgc ttaccgagta tgcggatttt ttccgccagg tcgagtcttc accgctgaat
     3301 ccggcgcagg cccgggcagt cgttaatggc gagcattctc tgttagtgct ggctggtgca
     3361 ggaagcggaa aaacgtcggt gctggtggcc cgtgcaggct ggttgctggc gcgtggtgaa
     3421 gcgtcccctg agcaaatttt attgctggcg tttggtcgca aagccgctga agagatggac
     3481 gagcggattc gcgaacggct acataccgaa gacattaccg cacgcacgtt tcatgcgttg
     3541 gcgctgcata ttattcagca gggcagcaaa aaagttccga tagtcagcaa actggaaaat
     3601 gataccgctg cccgtcatga actctttatt gctgagtggc gcaagcaatg cagcgaaaag
     3661 aaagcgcagg cgaagggctg gcggcaatgg ctgacggaag aaatgcagtg gtcagtgcca
     3721 gaaggtaact tctgggatga tgaaaaatta cagcgtcgcc ttgcctctcg cctcgatcgc
     3781 tgggtaagtc tgatgcggat gcacggtggt gcacaggcag aaatgattgc cagtgcaccc
     3841 gaagagattc gcgatctgtt cagtaaacgt atcaagttga tggccccgtt attaaaagcc
     3901 tggaaaggtg cgctgaaggc agaaaacgct gtcgattttt cgggccttat tcatcaggcg
     3961 attgtgattc tggagaaagg ccgctttatc agcccgtgga agcatattct ggttgatgaa
     4021 tttcaggata tctcgccgca gcgcgcagcg ttgttagcgg cattacgcaa gcaaaacagt
     4081 cagacgacgt tgttcgccgt tggtgatgac tggcaggcga tttaccgatt cagcggtgcg
     4141 caaatgtcgc tcaccaccgc tttccatgaa aactttggtg aaggcgatcg ctgtgattta
     4201 gacacgactt accgttttaa cagtcgtatc ggtgaggtgg caaaccggtt tattcagcag
     4261 aacccaggcc agctgaaaaa gccgctaaac agcttaacca atggagacaa aaaagccgtc
     4321 acgttattgg atgagagtca acttgacgct ttgctggata agctctctgg ttatgccaaa
     4381 ccggaagagc gcattctgat cctggcgcgt taccatcaca tgaggcctgc cagcctggaa
     4441 aaagcggcaa cacgctggcc gaagttgcaa atcgacttta tgaccattca tgccagcaaa
     4501 gggcaacagg cggattacgt catcatcgtt ggcttgcagg aggggagtgg tggttttccg
     4561 gctgcggcgc gggagtcgat tatggaagag gcgctactgc caccggttga ggatttcccg
     4621 gacgctgaag aacggcgttt aatgtacgtg gcgctgaccc gggcacgcca tcgggtatgg
     4681 gcactgttta acaaagagaa tccctctccc tttgtggaaa tactgaaaaa tctggatgtg
     4741 ccggtggcga gaaaaccgta agaaacaggt ggcgtttgcc acctgtgcaa tattacttca
     4801 gacggtcagc gagataacgc tgataatcgg ggataagaat atcgaccgcg tcgttgaaat
     4861 gcggcgactg gataatgaag tctgccgttg ccacgttggt ggcgaccgga atgttccata
     4921 ccgtcgccag acgcagcaag gctttcacgt caggatcgtg cggcacggca tttagtggat
     4981 cccagaagaa tatcaataca tcaattttcc cttctgagat caatgcgcca acctgctggt
     5041 caccccccat tgggccactc aacatcgcgt tgacgttcat gccggtcgcg cgggaaatca
     5101 agttaccggt agtgcctgtt gcatacagta cgtgttgttc cagtaacggt tgatgccgtt
     5161 ccacccagct catcagcatt tgtttgcagt gatcgtgtgc caccagcgca atatgtttcc
     5221 gcgcaggtaa agtgcgagtc gtcagttcca taatgtacat ccgtagttaa ctttcctaca
     5281 gattactgta agcacttatc gctgcaagat aaagaccgaa aaagcctgcg cacaggcaca
     5341 aaaatctcag gaagatggtt gtttttccgc ccattgcagg aaagtatttc gcgtttgtgg
     5401 gtcagccagt ttaaaccaat acttcagccg ttgttctgtg agcacctgag actgcggtgg
     5461 aatagcatcc agctcggaaa cacccgaaag cagcgtactg tcatctgcca tcatcgtggc
     5521 aaattgcggt agtgaagcgc gtttcgcgga tttgttatag cgttcaacct cgacctcata
     5581 atcaatcgtt tttgccgttg aggtaatggc gagaatatcc agctttaccg gaatcggcgt
     5641 cgcatcgcca tccaacaatt caaggcgcgg cgcggcatca aaatggttag cttcccgctc
     5701 attctccagg cgaggcaggc gaaaattgac ctggttgatg agctgggtat taaaactgac
     5761 caccagcggt ggggagatat acagccgttc ttcactattg gaaagatgaa tcgtcttctc
     5821 aacgcgaaac actaactgat gcggtccatt gtccagttca atgctgtccg caccgcgcag
     5881 cagagagctg gaaaccttct tgccgtcgag taccagaaga tcgacatcgg ttgaaagccg
     5941 cagcgtggtg gcaaaaacgg ataccggcaa acataatgca atcaaggtgg tcacgatgcc
     6001 ggttttcata ggtagctcct gtcaaaagac cgcccgaata ataatgtgtc aaaatttttc
     6061 tttaatcata cttactaaca tatagacata tttcccgctt ttgctctcat tcattcgtat
     6121 tagctgcatg gttggcatgt cggctttgtc gtacactttg ttaaacagtc tggaggaaat
     6181 aatgaaagaa accgatattg caggcatttt aacgtccact cacaccattg cgctggtggg
     6241 ggcaagcgac aaacccgatc gccccagcta tcgcgtgatg aaatatctac tcgaccaggg
     6301 gtatcacgtc attccggttt cgccaaaagt tgccggcaaa acgctgctgg gccagcaggg
     6361 gtatggcacg ctggcagacg ttccggaaaa ggtcgatatg gtcgatgttt tccgtaattc
     6421 agaagcggcg tggggcgtgg cacaggaagc tattgccatt ggcgcgaaaa ccctctggat
     6481 gcaactgggg gtgattaatg agcaagcggc agtactggca cgggatgccg ggttaaatgt
     6541 ggtgatggat cgctgcccgg ctattgagat ccctcgcctg ggtctggcca aataaaaaat
     6601 ccccggaagg caaaaacctt ccggggattt gttcagggat tagttacgca gacgcggggc
     6661 ctggagttgt ttgcggatgg tctgcgccag ctcatccatg gatggctgtt caggatgttc
     6721 atcctgcaat tcgctactta gctgggcttc agccaggtag gtatgaaccg gtaggccgtt
     6781 atcgtcctcc atcaccacgt gataccacgg agcggcgcga agctcgtcat tcaccgccag
     6841 ctcatcgggc gacggttcag agagcgaata aaccgggtcg atatccacga ccactccgag
     6901 ataacctaac agggaatggc ggacctgctg gccgataccg aatttgctgg caatcatagt
     6961 cacctcccgg gaaatcttta cctttgatat aagggtaaaa ttccacattt caagttacat
     7021 gacgcgacag gcaaaccctt tcagatatag cccttccggg taggtagcga tcaccggatg
     7081 atcggctgcc tgacggaact gctctataaa ttgtacatca cgaccggcat caattgcggc
     7141 atccgcgatg attttctgaa ataaatcgct ggtcatcaga ccggaacagg agaaagtcag
     7201 gagaataccg ccttcattca gcaactgaat cgccagcatg ttgatgtctt tataaccacg
     7261 acacgcgccc atcaactggc ttttattctc aacaaacttc ggcgggtcca tcacgataac
     7321 gtcaaatttt tcaccgcgat cgcgataagt acgcagcaat ttaaagacat catcacggac
     7381 aaactcagcc ttgctcagat ccagtttgtt cagctcaacg ttctgccgtg caatatccag
     7441 cgcttcctgg gaggtatcaa cgctgacaac ctggctgcaa ccgcccatca gtgccgatac
     7501 ggcgaaacca ccggtatagg agaaacagtt cagcacacgt ttattttcaa cgtagcggcg
     7561 ggtagccagg cggctgtctc gctggtccag atagtagccc gttttgtgtc cgtgctgaat
     7621 atccaccagc agcttcatgc cgtgttcttc aatcggcagc agggcaggcg gcaactcgcc
     7681 ggtgacgagg ccctgggtca gctccatccc ttctttttta cgtaccgcaa catcgctgcg
     7741 atcgtaaatc gcacattccg ggtacagcgt ttgcagggca ctaattaatg ccgcgcgctg
     7801 atattctgcg ccagcactca gcagttgcag caccagaaaa ttaccgaaac gatcgatagt
     7861 aatacccggc aggccatcag attctccggc gattaaacga tagctgtcga ggccatcttt
     7921 ttgcgccagc cagtcacgcc atttttgtgc ttgttgcaaa cggcgggaaa aaaaagcaat
     7981 gtcgatagac tcagacgggt caaacgtcca gacgcgcgcc cggatttgcg aagctggcga
     8041 ataagcgccg cgtgctaacc attttccctg atgatcaaca atatcgatgg tttcaccgag
     8101 gctggcttta ccttccatgc gggcaacggc cccggaaaag acccacggat ggcgacgaag
     8161 taatgatttt tcgcgccctt tggctaacac taaacgtaca ctcataattt acttttctgt
     8221 cgatgcaaag aaatgggcgt cattgtccgg agttttccat ggatttgcaa cgcgtccatt
     8281 aaggataagg agagcagcaa tgtcgaaaat ctgcataatt gcctggattt acgggcgagt
     8341 tcagggcgta ggatttcgct acaccacaca gtacgaagcg aaaagactgg gcttaaccgg
     8401 gtacgccaaa aatcttgacg atggcagcgt ggaagtggtt gcctgcggtg aagaagggca
     8461 ggtagaaaaa ttaatgcagt ggttaaagag tggcggcccg cgttctgcgc gtgtagaacg
     8521 ggtgctcagc gagccgcatc atccctcggg ggaattaacc gattttcgta tccgttaaat
     8581 acattttacc ggttttggca ggccagcaat tttggtggct tgcttagccg ggcctttcgg
     8641 gaacagtcgg tataaatagc ggctgttacc tttttcttca ccaaatttat tcgccatcgc
     8701 ttttaccagc atacgaatcg ccggagacgt attaaattcc aggtagaagt cacgtacaaa
     8761 acgcaccact tcccagtgtt ctggcgacag cgaaatccct tcgttctctg caatcaccac
     8821 tgccagcggt tcactccact ggctgctttc tttgagatag ccttcggtat ccgtttctat
     8881 ctctttacct tcgaagatca gcataattat tactacctta atcagactgg cggcagtgta
     8941 acaaaaaaca aagccccgca taaagcgagg ctatgaaagt gttagcgggt gagattaatc
     9001 gcggctggcg aagcccagaa tgctcagtag gctgacgaag atgttgtaca gcgaaacata
     9061 caggctaacc gtggcacgaa tatagttcgt ttcaccgcca tgaatgatgt tgctggtttc
     9121 aaacaagata gcgccagagg agatcagaat gaagaccgcg ctgatcgcca gatgcagagc
     9181 aggcagttgc aggaagatat tagcaatcat accaatcagt accaccacaa tacccgccat
     9241 cagcataccg ccgaggaacg acatatcttt gcgggtggtc agcacatatg cagagcagca
     9301 gaagaacact aacgccgttc cgcccagtgc catagcgatt acgtcaccca ttccggcaga
     9361 cagataggtg ttcagaattg gtccgaggat ataacccaga aaaccggtaa aggcgaatgc
     9421 ggagataatc ccggtcggct tattcgccgt tttataggtc aggaacatca aaccatacat
     9481 acccaccaga gtcagaatca gacccggaga tggcagcatc agcaccgtac tggcagttgc
     9541 ggtaatcgcc gaaaaggcca gcgtcaggct cagcagaaaa taggtattac gcagcacctt
     9601 atgggtgcta agcagtgatg tacggtcatg tgaagaacta acaatacgat ccattgagtc
     9661 actctctatg acagatgtaa ttaattaagc agcataatga taatgcgtaa gggcacccag
     9721 aagttttacc catctttacg catttgatct ggaacaggtt taacagcgga ttatcaggtc
     9781 attaagcaaa tataacgccc tgagaattcc gacaggcaaa agaaaaaggg ggtagcgttt
     9841 agctaacccc ttatcttatt tggcggaagc gcagagattc gaactctgga accctttcgg
     9901 gtcgccggtt ttcaagaccg gtgccttcaa ccgctcggcc acacttccgg aatgacgcgc
     9961 actataaaca tcccgatgcg gcgtgtaaac ccctaatttg tttgtttgcc tgaaaaacag
    10021 ccaaaagtgc attgatagcg tgaaataaca gcagattgat catttcatca ccatgaattc
    10081 cttctctttt actcgtttag caaccggcta aacatcccca tcgcccggct aaagaaaaat
    10141 aggtccattt ttatcgctaa aagacaaatc cacacagttt gtattgtttt gtgcaaaagt
    10201 ttcactacgc tttattaaca atactttctg gcgacgtgtg ccagtgcaga aggatgagct
    10261 ttcgttttca gcatctcacg tgaagcgatg gtttgccttg ctacagggac gtcgcttgcc
    10321 gaccataagc gcccggtgtc ctgccggtgt tgctaggaga agagacgtgc gatatgaata
    10381 acgaggaaac attttaccag gccatgcggc gtcagggcgt tacccggcgc agctttctca
    10441 aatattgtag tctggctgcc acgtcgctgg gattaggcgc gggaatggca ccaaagattg
    10501 cctgggcgct ggagaacaaa ccgcgcattc cggtggtatg gatccacggt ctggaatgta
    10561 cctgctgtac cgaatctttt atccgctccg ctcacccact ggcgaaggac gtcatccttt
    10621 ccctgatttc cctcgattac gacgataccc tgatggctgc cgccggaacc caggcggaag
    10681 aagtctttga agacatcatc acgcaatata atggcaaata tatcctcgca gtagaaggta
    10741 atccgccgct gggcgagcag gggatgttct gtatcagcag cggtcgaccg tttattgaga
    10801 aactcaaacg tgccgctgcc ggagccagcg cgattatcgc ctggggaacc tgcgcgtcct
    10861 ggggctgcgt gcaggccgcg cgacctaatc cgacacaggc aacgcctatc gacaaagtca
    10921 tcaccgacaa acccattatc aaagtacctg gctgcccgcc gatcccggat gtgatgagcg
    10981 ccatcattac ttacatggtg acctttgatc gcttgccaga tgtcgacaga atgggtcgtc
    11041 cactgatgtt ttatggtcag cgtatccacg ataaatgcta tcgccgcgct cacttcgacg
    11101 ccggagagtt cgtccagagt tgggatgatg acgctgcccg caaaggttat tgcctgtaca
    11161 aaatgggctg caaagggcct accacctata acgcctgttc ctccacacgc tggaatgatg
    11221 gcgtttcttt cccaatccag tctggtcacg gctgcctggg ctgtgcggaa aatggtttct
    11281 gggatcgcgg ttcgttctac agccgcgtgg tcgatattcc gcaaatgggt actcattcca
    11341 ccgccgatac cgtcggttta accgcgcttg gcgtggtggc agcagctgtt ggtgtgcacg
    11401 cagttgccag cgccgttgac caacgcagac gtcataacca gcaacctaca gaaaccgaac
    11461 atcagccagg caatgaggat aaacaggcat gagcactcag tacgaaactc agggatacac
    11521 catcaataat gccggacgcc gcctggtggt cgacccgatt acgcgcatcg aaggccacat
    11581 gcgctgtgaa gtgaatatta acgatcagaa tgtgatcacc aatgccgtct cctgcggcac
    11641 catgtttcgc gggctggaga tcatcctgca agggcgcgac ccgcgcgatg cgtgggcgtt
    11701 cgttgaacgt atctgcggcg tctgtactgg cgtacacgcc ctggcttcgg tttacgccat
    11761 cgaagatgcc atcggtatta aagtgccgga caacgccaat atcatccgca acattatgct
    11821 ggcaacgctc tggtgccacg atcatctggt gcacttctat cagcttgccg ggatggactg
    11881 gatcgacgtg ttagatgcgc tgaaagccga cccgcgaaaa acctccgaac tggcgcaaag
    11941 tctctcctct tggccgaaat catcccctgg ctatttcttc gacgtacaaa atcgcctgaa
    12001 gaaattcgtt gaaggcgggc agttggggat cttccgcaat ggctactggg ggcacccgca
    12061 gtacaaactg ccgccagaag ccaacctgat gggctttgcc cactatctcg aagctctcga
    12121 tttccaacgt gaaattgtca aaattcacgc ggtctttggc ggtaaaaacc cgcatccaaa
    12181 ctggattgtc ggtgggatgc cttgcgccat taacattgac gaaagcggcg cggtcggggc
    12241 ggtcaatatg gaacgcctga acctggtgca gtcgattatc acccgcacgg cagacttcat
    12301 taacaacgtg atgatccccg acgccttagc catcggtcag ttcaacaaac cgtggagcga
    12361 aatcggcacg ggtctttctg ataaatgtgt cctcagctac ggcgcattcc cggatattgc
    12421 caacgacttt ggtgagaaaa gtctgctgat gcctggcggc gcggtgatta acggcgactt
    12481 caacaatgtg ctgccagtgg atttggttga tccgcagcag gtgcaggagt ttgtcgatca
    12541 cgcctggtat cgttatccca acgatcaggt cgggcgtcat ccattcgatg gcattaccga
    12601 cccgtggtac aaccccggcg atgtcaaagg cagcgatacc aacattcagc agctgaatga
    12661 acaggaacgc tactcgtgga tcaaagcgcc acgctggcgc ggtaacgcga tggaagtggg
    12721 gccgctggca cgcacgttaa tcgcttatca caaaggcgat gctgcgaccg ttgagtcggt
    12781 cgatcgcatg atgtcggcgt tgaacctgcc gctttccggt atccagtcaa cgttgggccg
    12841 cattttgtgc cgcgcgcacg aagcgcagtg ggccgcaggt aagttgcagt atttcttcga
    12901 caagctgatg accaacctga aaaacggcaa tctcgccact gcttccactg aaaaatggga
    12961 acctgcaacc tggccgacag agtgccgtgg cgtcggcttt accgaagcgc cgcgcggagc
    13021 gttaggccac tgggccgcca ttcgcgatgg caagattgat ctctaccagt gcgtggtgcc
    13081 gaccacctgg aacgccagtc cgcgcgatcc caaagggcag attggcgctt atgaagcggc
    13141 gctgatgaac accaaaatgg cgatccccga gcaaccgctg gagatactgc gtactctgca
    13201 cagctttgat ccgtgcctcg cctgttcaac tcatgtgctg ggcgacgacg gtagcgagct
    13261 gatctccgtg caggtgcgtt aacagcgaag gagaatcatc atgcaacaga aaagcgacaa
    13321 cgttgtcagc cactatgtct ttgaagcacc agttcgtatc tggcactggc tgacggtgtt
    13381 atgcatggcg gtgttgatgg tcaccggtta ctttatcggc aagccgctac cttccgtcag
    13441 tggcgaggcg acgtatctgt tctatatggg ttacatcagg ttaattcact tcagcgccgg
    13501 gatgattttt accgtggtct tgctgatgcg gatctactgg gcttttgttg gcaatcgtta
    13561 ctcccgcgag ctgtttatcg tgccggtatg gcgtaaaagc tggtggcagg gcgtgtggta
    13621 tgaaatccgc tggtatctgt ttctggcaaa acgtccgagt gccgatatag gccataaccc
    13681 catcgcccag gcggcgatgt tcggctattt cctgatgtcg gtctttatga tcatcaccgg
    13741 ttttgcgctg tacagcgaac acagccagta cgctattttt gcgccgttcc gttatgtggt
    13801 ggaatttttc tactggacgg gtggcaactc aatggacatt cacagctggc atcggctggg
    13861 gatgtggctg attggggcgt ttgtgatcgg tcatgtctac atggcgctgc gtgaagacat
    13921 catgtccgac gacacggtga tctccaccat ggtcaacggc taccgtagcc acaaatttgg
    13981 caaaataagt aacaaggagc gttcatgagc gagcaacacg tggtggtcat ggggctgggc
    14041 aacctgctgt gggccgatga aggcttcggc gtgcgggtgg cggaacggct gtatgcccat
    14101 taccactggc ccgagtatgt ggagattgtc gatggcggta ctcagggact gaacttgctg
    14161 gggtatgtcg aaagcgccag ccatctgttg attctcgatg ccattgacta cgggctggaa
    14221 cctggaacgc tgcgaaccta tgccggagaa cgcattccgg cctatctcag cgcgaagaaa
    14281 atgagcctgc atcagaacag tttctccgaa gtgctggcgc tggcagatat ccgcggacat
    14341 ctgccagcac atattgccct cgtcgggctg caacccgcga tgctcgacga ctacggcggc
    14401 agcctgagcg aactggcacg ggagcaactg cccgctgcgg aacaatcggc gctggcgcag
    14461 ttggctgcgt ggggaagtgt gccgcaaccg gctaatgaat cgcgttgtct caattatgac
    14521 tgtctgtcga tggaaaatta cgaaggcgtt cgcttgcgcc agtaccggat gacacaggag
    14581 gagcagggat gagcaacgac acgccatttg atgcgttgtg gcaacgaatg ctggcgcgcg
    14641 gctggacgcc ggtcagtgaa tcccgtcttg acgactggct tacgcaagcg ccagacggcg
    14701 tggtgttatt aagcagtgac ccgaaacgca cgccagaggt cagcgataat ccggtaatga
    14761 ttggcgaatt actgcacgag tttcccgact atacatggca ggtggcgatt gctgaccttg
    14821 agcagagcga agccatcggc gatcgctttg gcgcctttcg ctttcctgcc actttagtgt
    14881 ttaccggcgg aaactatcgc ggcgtgctga atggtattca cccgtgggcg gaactgataa
    14941 acctgatgcg cgggcttgtc gaaccgcagc aggagcgtgc ctcatgagcg aaactttttt
    15001 ccatctgctg gggccaggaa cgcaaccgaa cgatgacagt ttcagcatga atccactgcc
    15061 gatcacctgt caggtgaatg atgaaccgag tatggcggcc ttggagcaat gtgctcacag
    15121 cccgcaggtg attgcgctgt taaacgagtt acaacatcaa ctaagcgaac gccagccgcc
    15181 gttgggcgag gtgctggcag tcgatctgtt aaatctcaac gccgacgatc gtcactttat
    15241 caatacgctt ctcggggaag gggaagtgtc agtgcgcatt cagcaggctg acgacagtaa
    15301 aagtgaaata caggaggcga tcttctgcgg attatggcgg gtgcgcagac gtcgcggcga
    15361 aaagttgctg gaggacaaac tggaggctgg ctgcgcgccg ctggcgttgt ggcaggcggc
    15421 aacgcaaaat ctcttgccga cagattcgct gttaccgccg cccattgatg gcctgatgaa
    15481 tggcctaccg ttggcgcatg agttactggc acatgtacgt aaccccgacg cgcagccgca
    15541 cagcattaat ctgacgcaat tacccatcag cgaggctgat cggctttttc tctcgcgtct
    15601 ctgtgggccg ggaaatattc agattcgtac cattggctat ggcgagagct atatcaacgc
    15661 cacggggtta cgccatgtct ggcatttacg ctgtacggac accttaaaag gcccgttact
    15721 ggaaagttat gaaatctgcc caataccgga agtggtgctg gcagcgccag aagatttggt
    15781 cgactctgcg cagcggctta gcgaggtatg tcagtggctg gcggaagctg caccgacgta
    15841 aaaagacggt aagtatcgct ttcagtctta tgaatatcgc aatcggcgaa tacctctggt
    15901 cgtagagttt caggataaag agggagatct aacattatcg ggttattttt ctctcttcgc
    15961 ctacaggagt gcgcatgtgg gatgtcattg atttatcgcg ctggcagttt gctctgaccg
    16021 cgctgtatca ctttttattt gtacccctta ccctggggct gatttttttg ctggctatca
    16081 tggaaaccat ttacgtggtt accggcaaaa caatctaccg cgatatgacg cgcttctggg
    16141 gtaagctctt cggtatcaat tttgctcttg gcgtggctac cggcctgacc atggagtttc
    16201 agtttggtac taactggtca ttctattcca actatgtggg cgatattttt ggcgcaccac
    16261 tggcgatgga agcattaatg gccttcttcc tcgaatccac ctttgtcggg ctgttcttct
    16321 tcggctggca acggctgaat aaataccagc acctgctggt gacgtggctg gtggcgttcg
    16381 gttcaaatct ctctgcattg tggatattga atgccaacgg ttggatgcaa tacccgaccg
    16441 gtgcgcattt tgatatcgac accctgcgta tggagatgac cagcttcagc gagctggtct
    16501 ttaacccggt cagccaggtg aaatttgtgc acaccgtcat ggcgggctac gtgaccgggg
    16561 ccatgtttat tatggcgatc agcgcctggt atttactgcg cggacgggag cgcgatgtcg
    16621 cattacgctc gtttgccatc ggttccgtct tcggtactct ggcgattatc ggtaccctgc
    16681 aactcggaga cagttctgcg tatgaagtcg cgcaagtaca accggtaaaa ctggcggcga
    16741 tggaagggga gtggcaaacg gaacctgctc ctgcaccgtt ccatgtggtt gcctggccgg
    16801 aacaggatca agagcgtaac gcctttgcca tcaaaattcc cgcgctgcta gggatcctcg
    16861 ccactcactc attagataaa cccgtgccgg gtctgaagaa tttgatggct gaaacctacc
    16921 cacgcttgca acgcggacgt atggcctggc tgttaatgca ggaaatatct caaggcaatc
    16981 gtgagccgca tgtgttgcag gcattccggg agctggaagg cgaccttggt tacggcatgt
    17041 tgctctcccg ctatgcgccg gatatgaatc atgtcacagc cgcacagtac caggcggcga
    17101 tgcgtggcgc gatacctcag gttgcgccgg tattctggag tttccgcatc atggtgggct
    17161 gtggttccct gctgctactg gtgatgctga ttgcgcttgt ccagacgctg cgtggcaaaa
    17221 tcgaccagca tcgctgggtg ctgaaaatgg cgctctggag tttgccgttg ccgtggattg
    17281 cgattgaagc cgggtggttt atgaccgagt ttggtcgtca gccgtgggcg atacaggaca
    17341 tcttaccgac atactccgcg cactccgctt taaccacagg acaactggct ttctcactga
    17401 tcatgatcgt agggctttac accctgttct taatcgccga agtctacctg atgcagaaat
    17461 atgcccgtct ggggccgagc gcgatgcaga gtgaacaacc gacgcagcaa caggggtaaa
    17521 ggagaaaatc atgtttgatt atgaaacatt gcgcttcatc tggtggctgc tgattggcgt
    17581 gatcctggtg gtctttatga tctccgacgg atttgacatg gggatcggct gtttgctgcc
    17641 gctggtggcg cgtaatgatg atgaacgccg gatagtgatt aatagcgttg gtgcgcactg
    17701 ggaaggcaat caggtctggt tgatcctcgc aggtggggca ttatttgcag cctggcccag
    17761 agtgtatgca gcggcgtttt ccggctttta tgtggcgatg atcctggtgc tgtgctcgct
    17821 gttcttccgt ccgctggcct ttgattatcg cggaaaaatt gccgatgcac gctggcgtaa
    17881 aatgtgggat gccggtctgg tcatcggcag tctggtgccg ccggtagtct tcggtatcgc
    17941 cttcggcaac ttgttgctcg gcgtgccgtt tgccttcaca ccgcaattac gcgtggagta
    18001 tctcggcagc ttctggcaac tgctgacgcc attcccttta ttgtgtggtt tgctcagcct
    18061 tgggatggtg attttgcaag gtggcgtctg gttacaactg aaaactgttg gtgtgattca
    18121 tctgcgttca cagctggcga ctaaacgcgc tgcactgttg gtgatgctgt gctttttgct
    18181 ggcaggttac tggctgtggg tcggtattga tggctttgta ctgctcgccc aggatgctaa
    18241 cggtccttcc catccgttaa tgaaactggt ggcagtgcta cctggtgcct ggatgaataa
    18301 tttcgtcgaa tcgcccgttt tgtggatctt cccgctgctg gggttcttct gcccattgct
    18361 gacggtgatg gcgatttatc gtggtcgccc gggttgggga tttttgatgg catcattgat
    18421 gcaatttggc gtgattttca cggcaggcat cacgctgttc ccctttgtca tgccgtcaag
    18481 tgtgagtcca atctccagcc tgacgttgtg ggacagcact tccagtcagc tgacgctgag
    18541 cattatgttg gtaatcgtgc tgatattttt gcccattgtg ttgctctaca ctctctggag
    18601 ctactacaaa atgtggggac gcatgacaac agaaactctc cgccgtaaca aaaacgagtt
    18661 gtactaagga gcaggaacat gtggtattta ctttggttcg tcggcatttt gttgatgtgt
    18721 tcgctctcca cccttgtgtt ggtatggctg gacccgcgtc tgaaaagtta acgaacgtag
    18781 gcctgatgcg gcgcattagc atcgcatcag gcaatcaata atgttagata tgaaaagcgg
    18841 aaacatatcg atgaaagcga tcttaatccc atttttatct cttctgattc cgttaacccc
    18901 gcaatctgca ttcgctcaga gtgagccgga gctgaagctg gaaagtatgg tgattgtcag
    18961 tcgtcatggt gtgcgtgctc caaccaaggc cacgcaactg atgcaggatg tcaccccaga
    19021 cgcatggcca acctggccgg taaaactggg ttggctgaca ccgcgcggtg gtgagctaat
    19081 cgcctatctc ggacattacc aacgccagcg tctggtagcc gacggattgc tggcgaaaaa
    19141 gggctgcccg cagtctgctc aggtcgcgat tattgctgat gtcgacgagc gtacccgtaa
    19201 aacaggcgaa gccttcgccg ccgggctggc acctgactgt gcaataaccg tacataccca
    19261 ggcagatacg tccagtcccg atccgttatt taatcctcta aaaactggtg tttgccaact
    19321 ggataacgcg aacgtgactg acgcgatcct ctgcagggca ggagggtcaa ttgctgactt
    19381 taccgggcat cggcaaacgg tgtttcgcga actggaacgg gtgcttaatt tcccgcaatc
    19441 aaacttgtgc cttaaccgtg agaaacagga cgaaagctgt tcattaacgc aggcattacc
    19501 atcggaactc aaggtgagcg ccgacaatgt ctcattaacc ggtgcggtaa gcctcgcatc
    19561 aatgctgacg gagatatttc tcctgcaaca ggcacaagga atgccggagc cggggtgggg
    19621 aaggatcacc gattcacacc agtggaacac cttgctaagt ttgcataacg cgcaatttta
    19681 tttactacaa cgcacgccgg aggttgcccg cagtcgcgcc accccgttat tggatttgat
    19741 catggcagcg ttgacgcccc atccaccgca aaaacaggcg tatggtgtga cattacccac
    19801 ttcagtgctg tttattgccg gacacgatac taatctggca aatctcggcg gcgcactgga
    19861 gctcaactgg acgcttccag gtcagccgga taacacgccg ccaggtggtg aactggtgtt
    19921 tgaacgctgg cgtcggctaa gcgataacag ccagtggatt caggtttcgc tggtcttcca
    19981 gactttacag cagatgcgtg ataaaacgcc gctgtcatta aatacgccgc ccggagaggt
    20041 gaaactgacc ctggcaggat gtgaagagcg aaatgcgcag ggcatgtgtt cgttggcagg
    20101 ttttacgcaa atcgtgaatg aagcacgcat accggcgtgc agtttgtaat gcataaaaaa
    20161 gagcattcag ttacctgaat gctctgaggc tgatgacaaa cgaagaactg tctaatgcgt
    20221 agaccggaaa aggcgttcac gccgcatccg gtcactttca gttttactct ttctcggagt
    20281 aactataacc gtaatagtta tagccgtaac tgtaagcggt gctggcgcgt ttaatcacac
    20341 cattgaggat tgcgccttta atattaacgc cagcctgttc cagacgctgc attgacaagc
    20401 tcacctcttt ggcggtgttc aagccaaaac gcgcaaccag caggctggtg ccgacagagc
    20461 gtcccacgac cgcagcattg ctcaccgcca gcatcggcgg cgtatcgaca atcaccagat
    20521 cgtaatggtc gttcgcccat tccagtaatt gacgcatccg atcgcgcatc agcagttcag
    20581 acgggttagg tggcacctga ccgcgagtaa tcacatcaaa gtctcctttg ccaaaatgct
    20641 ggatcacttt gttgagctca tctttacctg ccagatattc cgacaagcca tgttcattac
    20701 tcacggtaaa caggttatgc gaataaccac ggcgtaagtc ggcatcaata aataacactt
    20761 tttgatcgga ctgggcgatc accgctgcca gagttgaact gacaaacgtt ttaccactgt
    20821 ctggcgtcgc accggtgatc atcagaatgt tattctcagt ctccatcata gcgaaatgca
    20881 gactggttcg tagcgcacgt acggcttcca cagcagaatc cgccgggtta tccaccgcca
    20941 ggaaggggat atttttagta cgatggcgct gctgattaga aaataaattt ttcttacgca
    21001 gacgggtgcg tttatccagc cactcggaca ttgggatagt ggaataaacg ctgatgccgt
    21061 gctcttccag ttgttccggg gcttctacac cacgacgcaa catcgcacgc gccagcacgg
    21121 cactcaccga aataaacagg ccaagaataa aaccaagcac cacattcaac gcttttttcg
    21181 gtttcactgg ctgcggctga gtgactgccg ggtcgataat ccgcacgtta ccaatggcac
    21241 tggatttcga aatactcaac tcctgctggc ggttaagtaa ttgcagatat accgcacggc
    21301 ccgcttctac gtcacgactt aaacgcaaca cttcctgttg ggtggaaggc attgccgata
    21361 cccgcttatt caggcgtttg cgttcttgct ccagcgtctg gcgtttttcc agcagcgcac
    21421 gataagttgg gtgatctttc ttatacagct gggagatctc tgcctcgcgg aaggtcagct
    21481 cattgagttg attatcaacg ttcacaatct gctcaagaac ggctttggct tccaggttaa
    21541 ggtcaaccga atcgcgctgc tggcgataaa cgttgagttt ttcttccgcc tggtccagct
    21601 cgctgcgcac ttcaggtaac tggcgctgta agaattcaag gctttgtgaa tcctgcgccg
    21661 cctggcgagc gatattctgt tgcaaatagt tgttagcgat gctgttcaga atacgagtaa
    21721 tcaactgggg atcatcacca gtcatggtaa gttccagcat cccgctttct ttactgcgtt
    21781 cgctaacggt aaaggtttcc tgcaatgcgt taatcgcttc cagttcggta cgctggctca
    21841 ggacaaactg tgttcctggt ttggccttaa tgtccgcgat agtcagcgca acgccatctt
    21901 tttccagacg ctggccgacc ataccattga cggtgaactc ttcaccttcc agtgtatagt
    21961 ggccgttttc cccaaccgtg agtgtcagtt gctgatcctg accattcagt tgtggaatat
    22021 gcatccagct gatcgccagc tcacctagtt tttctttggt taatctcgcc cagccgcgac
    22081 ccacaatcgg aaaatacttc tgctcaacta tgtcgcgcag attcagttca gcaatggttt
    22141 taccgagaat catgcgcgat tgcagcagtt ggatctccgg tgcagactcg ggcgatgagt
    22201 tagggatcat atcgctcagg ccgctgagaa tggcgttgcc ctgtttttgc tcaacctgga
    22261 ccagagtatc tgcctgataa attggtgtgc ttaacagcga tcagcgtgaa taacgcggtc
    22321 acgctgataa taaacttacg gtgatcccat aactcgccga ccagacgaag cagatcgatc
    22381 tcattttcct gagtgctgcc tggtggcgta ttcatatttt tagttgtcat cgtattttat
    22441 aaactcaaat taccggctga ggcgcttcgc ccattcctga ctggcgcgct ccaacatact
    22501 gtagacatgt tcaaatgcgt cctgactttt acgataggga tccgggatct ctttctgttc
    22561 cagccattgc ccaaacagca ttgtcttccc gcggacctcg ggagcaattg cggtaacctg
    22621 agcaatatgt tccgactcca ttgccagaat cagatcgtaa tttcgtgcca tctcagcagt
    22681 gagcttacgt ccggcatgtc cttcaagaga tacgccatga ttagcggcaa catctgccgc
    22741 tgtcgcatct gcagggtgtt ttaccagacc atgaacacct gccgatttga cttttacgcc
    22801 cggcagacgt ttacgcagca agcgctcgcc aatgggcgaa cggcaaatgt tgccggtaca
    22861 aaccactagg attgagttaa attttagttg ggccatgttt gaatccaccg tgaagtctct
    22921 gtcaggtcat gcaccccgga aatcgtcggt accagcagag aaatcacacg gttccaacga
    22981 gccagcggcg cggtagtgac ataaacaatg tcgtaaggtt caagctggaa ttccgttccg
    23041 agtatcattg ccgatgcgtc cttcgcattc agctgataaa tattggcgat tttgccattc
    23101 tgcttgtttt gcgttgcacg aataacaaaa atccccgtcg catcagcgac atcctgattc
    23161 ataccctcag cgttacccag cgcttctgcc agggtcatac cgctgcggtc cattttcagc
    23221 gtgctctgtt tacccacttc acccatcacg aagactttca gatcatcgtt gcgcggaata
    23281 aataaaatat cgcccgggtg cagcagtttg ttctggcgca agtcgccacg ctgcatcagc
    23341 gcatagaggt tcactttggt ctttacgcca ttctgggtga gcaccacgtt gcgccagtct
    23401 gcatctgcgg tcagaccgcc cgcggcgtta atcgcatcca tgatggtcag aggaatattg
    23461 gtaatcggct gctgacccga tttgctgact tcgccggtga cgtacgcttt ttgcgatcgg
    23521 aacgccgcga cgctgacatc gacttgcggg ctttcaatga ctgaatcgag acgcgctgta
    23581 atctcgttgc gtacctgggt aagtgttttc ccggccactt tcaggcgacc aatatagggg
    23641 tagaaaatcg caccatcggc gttaacccag ttgccggtat cactggcgct acggtattgc
    23701 cctgcggggg tggttagttc cgggtgatcc cagaccgtaa ccatcaacac atcaccaatc
    23761 ccgatacgat attcatagtt tgcgatttgc tgctccagct ccgggttagc ctgcgacata
    23821 atcggtttgg cgcgcagctg atcaatcaat cctggcgtga cgggatagat attgaccatt
    23881 ttgtccaggt catactgatt gtcaggtagt tcaatgacat ccttgttact ggtcgataaa
    23941 ttctgaccag gaacaagggt acatgctgtc aaagacagaa cagccaatgt cagtaccgaa
    24001 aacttaaaaa tgttcttttt cattggttta tatttatcag tcaatgtaat gttatgaatt
    24061 acgtcaggtt attgtccaac cggtacttct cgctctgccg tcattggatc gagctggtat
    24121 ttgcgtgaca gcatttgtcc accatcacgc gtcaatggtg tccacgaaac caccgcgcgg
    24181 ttgcggttag gtccaatggt catcaagtcg aacggaatcg agatataaaa acctttacta
    24241 aagccgcctt cgccgtagtc atctttagat acgttactga ttgccgccca tacccctacc
    24301 gccacgccgc tgtcaaagag tttggcgacg tcgatcgttg cgcctttatc ttttgccaga
    24361 tattgcccaa cgctaagttt catcagtacg ccattgagcg tcggcgggtt ccagtaagcc
    24421 gtcacgaaac ctgtgggcgt ggaataatcg gtgaagcgca tcatgttgtc ccagtcacgc
    24481 tgcttcacgt agttaacgtc cacacccagc gcccagctgg catctagcgg gcgataaaga
    24541 agctcggaac cgacaccggc atacatcgtt tccagataac cgccatacac ctggccatag
    24601 aaaccattgc ctaagtcggc aaagtaattc gcttgcaagt tgttgagata aacgtcattg
    24661 cgaacgtaat cacggatatg cgtgcgcacg cggggcaggg tagagtccgc gggtaacagt
    24721 gaagacttaa acttgtcgta gttgttgtaa atattggtga aaataccgtc atcaagcagc
    24781 aggtggtcgg taaaccagta gcgggcgctg gacatcagcc ccagctggaa catatagaaa
    24841 tcttccgggc cgcccagcga ctgtgaaagt gttgggttga aagagtagct aaagcgatct
    24901 tcacgaatac ggtagcaccg accaaaggca gaaagatctt ctgcttcaac acgttgttgt
    24961 tgcagttgct ctgatttacc aggcgctgtg ccggccagct gtttgcgcag gctggctacg
    25021 tcggtttcgg tagtcaccat cgccatatgc tcgcgctttt gcgtcacgct aatcttctca
    25081 acgccttgcg gcaggttatt caccagaatc cggttggcac gatcgaccgc ttcgcgagag
    25141 tcacggtatt tgtattgctg accagacata tacagcgtct tatcgcgtag ctgaatttct
    25201 ggcgcgtcaa agcccgcgtt atacttcagg gcggtaagtt ggtttgctac cgtggtgtac
    25261 tgcaaacctt cagactctgg cgcaggctgc caggccggtt ttggcgtatc gcgtagggca
    25321 gggcgcagat cgttgaaatt ggtccgtaaa gtgaaaccaa acatcaacgt gtttccgcgt
    25381 tcataactga ggttgaggtc tgcccagctg gcagcgcgat aaactgcacc gacgttgaaa
    25441 tggcttgcct gaggcagttt gccagcgaaa tcattctggt aattgttgcc gtcgtattcg
    25501 agtttcagac gcaggggatt ccacggcgtt tgatactcaa tgccgccaaa gatggaagcc
    25561 gggccacgaa agatatcgct aaagctgata tcgcccgcat cgtgagactc tgcgcgatga
    25621 caatatttat cgctcacccg gcaaaacggg ttggtaatat tgcccgcatt accggcgtag
    25681 ccccatgcca tcccgagggt gaaatcaaat ggccccgctt gcttgctggc caccagatat
    25741 tcaccgtcaa acaaacccgt accagcaata tcacgtttac caaacgccac ttgcggtagc
    25801 cagtaacctt cttcccacag gcgaagttta aaatcgaatg atttgtcttt gtatgactga
    25861 tcgccgctga aatcttccca ctggctatat ttgcgtgtgc gcacatccgt ataacgaatg
    25921 gtgccttcca gccatgggaa cagcgccacg gaggtggagt agaaccggta ttgatcgtta
    25981 tcccggtagt tgacgctgaa ttcaccttcc ggtgcgatgc gcgcatttgg catctgcaac
    26041 aatcctgtgc cgccaaagtc tgattgcgac gaacccagcg gatccgggta ggttaatact
    26101 tcagcatgac aggcactgga gacggcaatg gccaggcagc ttaaaagata agaattcttc
    26161 ttcatcagtc aggaacacgt tgcgttaaaa cagacacaat ctggtcattt aaatcagcat
    26221 atttttcggg cagtacatgc gcagaaaagc ccaaccaaag ctggctgcca ggcggtggtt
    26281 ccacatgtct tttattccac agggcaacag gggcgacgac agtctcaccc tcaggcgtaa
    26341 tcaccataac gttattcttg tctgcgcctg caaggcgggg gtgatcttgc agataatcgg
    26401 taacgctacg gcctgcctgc cagggcaatt gcccagcccc agaaaccgca cccagcagag
    26461 taatagtcac cggacgctgt acggtataga gcgtatagtc cccgaccaac gggggattgc
    26521 tgttctcatc cactcgtaca aaatcagggt ccagtttgac cggtaagcga ccagtgatat
    26581 tgagattgag taactgctgg cgcacggatt taatcgttgc cgccacatcg tcgtcagctt
    26641 ctgcttccca ggaagcaagc tgtgccatga catgttggta gtctttaagc gctttagctt
    26701 tcgccgcaga gtcggtcagc aaggcaccag gccaccagag gcgatcgcgc agttgcggtt
    26761 gtgtcaccaa ctggacaaca ttttccacgg ggccaacgga aagtgtctgt tgttcgctag
    26821 gcagataaat agtcaccgtt ccttgcgcaa aggcatgggg tgtcattacg taaagtacgc
    26881 tggcaatgaa atacgactgt aatttattca tggtgcgggt ttaagaaacg tcatttctac
    26941 gggaataacc cctgcgccta acatttgccg actttgacga acctggccag tagccgaatc
    27001 gacccagaat gtgttttgcc actgtttttc tggcagcgtg gaagtcacct cttcctgcca
    27061 gacattacac agcacattgt gacccgcaat attcagcgtc tctaatccag caaaggaaaa
    27121 agtagaactt agggtcgcag agcgaaaatg gttgtcttca ctccagagaa tatcgcgcgt
    27181 ccagcggctg ccatctttta ttgccagcgc gttacgcaga ggggcccgcc cgggatttgt
    27241 cacttccagc agattattat taagtttgac ggtctttaaa agacgtccat tgtgggtaac
    27301 cagcatggcg ttatcctggg ataaccattt gctttgttct tgttctatat atcccagtac
    27361 cacaaagatt cgttgccctt cattaaggcg taaatacatc gtggaataag gcaacgcctg
    27421 gatctgctga gccgctacag tgatatcctg attatcgaaa aggctggcgc gaaatgtatc
    27481 gaccatactt tgctggctgt gcgtacatcc cgcaagaaat agtgcgaaaa tcgataaaat
    27541 aagagggcgc acctttaatc cttgaaaggt taattaaaaa aatgcaaatc tctaaagccc
    27601 ggtacgaata tcgcaaccgg gtattattta acttgaattt cgacgcgttt aagcagaatt
    27661 agcgcgtagt ggacgtggtg gttgaggtgt tatgagaacc gccatcgcca ctgtcatttg
    27721 ctgcaactac tgccaacgtc gccacaccaa cagccgttgc cacaccgaca actgtcgccg
    27781 tgccgacagc gccagcagcc acgccactag tggtagccgc agtcgtggtt gcacctgtag
    27841 tggtcgttgt ggtgccagta gtacccgtcg ttgcctcggt cccgtttgct gcttcagggg
    27901 cagcaaacgc agctggcgtc gtcaacatga aggccataag gattgcagaa agcttgtgtt
    27961 tcataacttt tcctttattc atcgcatgga caatacgtac gcttgagatc aggattgtct
    28021 actttgagta taacatcctt gtgaaatctc cctttcctca taccgctagc actatatact
    28081 gcgggtggga aaggcgcgca gagggaaata taagattgtt aacgatttcc ctctggaaat
    28141 acgacttgtg agcataagat aaatcttaga aaaatacgaa ttaaagataa gaaaattgtt
    28201 acgaaagcta ttaattattg ttggtaatat agtttcaagt ggtactataa ctggctgtta
    28261 aatggcattt ataactatta ggtgcagaga tattcgctta aatggattag tttcttagtg
    28321 gaaaagtaac tgatgttata atgtatggtg ttgactattg aactattcca ataaaaccag
    28381 tcagctttaa acaagcagcg tcatattaag agagataaac atttgccgct gttggtcctc
    28441 gcaggccatt tacgcggcaa aattccacgc gtaatcctgg tataagcact tctgcgtcgc
    28501 ggggagtgaa tgcggaaata tggacctgga cttctttacg accgtcggag gggataatga
    28561 atcctttgcc gcttttgcga tcaaaggttt tgacaattcc tgtcatttta cgggacaaac
    28621 aaattcctta ctgaaaatac tgcgctgcac tatacggggt taataaaata aagccagcga
    28681 tatttaagac cgccggacgg ctaaaataaa atttgcttaa tctcaattat catgccttaa
    28741 tagctgcgtc ggtttgaaag acagacagca tacaaagtag tttactaaag cagttctcat
    28801 tatcaggcat tatccccttc ttttgagtct ctctcctgaa cactaagtgg tttctgtatt
    28861 aaagccctgt ttgccgaaag gcccaaaatg aaggaagtaa aatatgtcta ataa
//
LOCUS       DAEBXN010000046        28676 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.046, whole genome shotgun sequence.
ACCESSION   DAEBXN010000046 DAEBXN010000000
VERSION     DAEBXN010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28676
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.046"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            1..648
                     /locus_tag="KJL99_002063"
                     /pseudo
     CDS             1..648
                     /locus_tag="KJL99_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163825.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            645..1031
                     /locus_tag="KJL99_002064"
     CDS             645..1031
                     /locus_tag="KJL99_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4436793.1"
                     /translation="MKFFCIIVIFLSINYIAIVNVFKSSAVAQSTKYYEENDIHDGSN
                     DIFFMKKYGPPAHEFVRPRYKFVGEYYIGLNQTYRITDPEFQDVPIKEMFWNLQGDLN
                     LTCWFHYKDGQWRVITYIFWPPGARF"
     gene            1056..3269
                     /gene="tssI"
                     /locus_tag="KJL99_002065"
     CDS             1056..3269
                     /gene="tssI"
                     /locus_tag="KJL99_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HBD4436794.1"
                     /translation="MSFVSTGNKPVGNGGPVITTPPIAGESGGMSTGSAVTDVSGAAE
                     EMAEQAAADLFGALPEPSGLVKAAVAAAQAAAAAAGISDMAGAVQDAAASLAAGAPGA
                     HNVTVSGSAVPPQMLLFAGMNGSEGLGNLFSYTVQLKTLDALNLGYVSPAANLPLQLM
                     VGKDLCVSIELDGGGKRYISGLVTAARVVGHESRSVTYELRIEPWLKLLTHTSDYKAF
                     QNKTVVDILDEVLDEYSFPVEKRLVENYPPRAWQVQYGETDFDFIQRLMQEWGIYWWF
                     EHSENSHTLVLVDAINGHKACPDSPLVEWHQEGLKLDKEFIHTITASERLRTGKWVMD
                     DFDFMKPRSLLKSTVASPRDTGHAEYEHYEWPGDYFTTGEGEMLTRIRMEAQRSPGSR
                     AHGAGHIRTLMTGYTFTLMNHPTAEINQEYLLVQTTLFLRDNAQHSGQDQHFTYVTTF
                     ELHPTSEVYRPQRTLSKPHTKGPQSAIVTGPAGQEIWTDKYGRVKVQFGWDRYGKNDE
                     NSSCWIRVSYPWAGKGFGMIQIPRIGQEVLVDFKNGDPDLPIIVGRTYNQDTMPPWGL
                     PGMASQSGIFSHSLQGGPTNGNMLRFDDKTGAEEVKFHAEKDLNTTVKNNETHTVMVD
                     RIKTIVKNETISVGEDRNATITKNDGLSVKLAQTINVGTTYRLDVGDQFTLRCGNAAL
                     VLHKDGSIEFCGKQLLLHTSDVMQLIGSGIDMNPDGGTAMTADDIAPLPTPDVSE"
     gene            3292..3737
                     /locus_tag="KJL99_002066"
                     /pseudo
     CDS             3292..3737
                     /locus_tag="KJL99_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233848.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
     gene            3759..4166
                     /locus_tag="KJL99_002067"
     CDS             3759..4166
                     /locus_tag="KJL99_002067"
                     /inference="COORDINATES: protein motif:HMM:NF017315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HBD4436795.1"
                     /translation="MYEAARVDDPIYHTSALAGFLIGAIIGIAIIAVASFAFFTCGFL
                     AGLILSFLADQIASGVLQLGEAIGRSIHSTVGKILTGSDNVSTNSRPAARAVLSTVIC
                     EDHSPEIRIAQGSGNIYINSQLPVRMTTLSVMQ"
     gene            4163..6451
                     /locus_tag="KJL99_002068"
     CDS             4163..6451
                     /locus_tag="KJL99_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HBD4436796.1"
                     /translation="MIEDGSPNVFLGGGTQTVLEISPEIPDWLRQVVDVLYVVAGMLG
                     GLAGAWRQAAKMGSKFGTKCAAKFIGGEMVGMAISDTVMGLFSNPVDVTTGQKILLPE
                     TDFTLPGRLPVTCSRFYASHLETEGLLGRGWRLNWEINLREDETYITFIGVQGRELSY
                     PKEMLIPGHQIFDPEEQFYLSRLHDGCYVLHYTDCSYYVFDEFDDHGVAPLLFMETPY
                     RQRIAFGRENGRLVRVASSSGHHLLLHRTMTQAGERLSHIELLKGGRPGNLVEYRYDD
                     NGQLTGVVNRAGVTVRQFAYENGLMTEHRNATGFTCTYHWEEIEGFPRVVEHTTSDGE
                     DYRFHYDFAGGQTVVTGRPEQKWQWWFDEETYVTAHRTPGGGMYRFTYNENHFPVAVE
                     LPGERRVTLEYDTLSRVVKETDPAGRVTQTQWNGSFAEITRRALDDDHVWKADYNEHG
                     QVIRETDPEGRVTRYGYDDQGLPETVCHPGKQQDRYTWNALGLLSSHRRITGSVQSWQ
                     YTQRGMLARHTDEEKRETRWQYTPEGLVASLSNGNGAQYRFSYDGDGRLTGEQRPDGL
                     IRMFALNADGFPVIIPTQGTEGGVRNEQQERDALGRLLRSDTQHSTRTFSYNRLDQIT
                     EVTLTPTEEGERLHHMQADTVRFAYDRSGWLTAEHSVHGSIKYRRDALGNPTDITLPD
                     GQHLSHLYYGSGHLLQTALDGITVSEYERDSLHRQVIRTQGKLATFSGYNADNRLSWQ
                     RSLPGGSQNPQQAVLPRRRTTA"
     gene            6448..7437
                     /locus_tag="KJL99_002069"
     CDS             6448..7437
                     /locus_tag="KJL99_002069"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4436797.1"
                     /translation="MTARDYIWNADGEVGGINDKLRGCLVFSYDRSGWLTSRTGQMYD
                     HDHYYYDKAGNLLTDEYQGAVMDNRLPGYGRDRYRYNEWGELTERRDQQLEWNAQGQL
                     TRVISSNSETRYQYDALGRRISKATSNLHTDRGERSRTTFVWEGFRLLQETTWQGKRT
                     YLYDAEQPYTPVAAITGRGESQKIWYYHTDLTGTVHEVTAPDGTLVWAGYQAGFGENR
                     GDISNSGAYFEQPQRLPGQYFDEETGLHYNLFRYYAPECGRFVSQDPIGLNGGLNLYA
                     YAPNPLGWIDPLGLNSLGIDSNNIFRGDGNYKGGGIGQPKSGTISAQDIIDHL"
     gene            7537..7740
                     /locus_tag="KJL99_002070"
     CDS             7537..7740
                     /locus_tag="KJL99_002070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4436798.1"
                     /translation="MAVSDLDDLVKNNQITVLTQEKVKAIFGLESSKKIRKELGNILQ
                     NMARNNEVLVHGQILGNKVSRCN"
     gene            7758..8078
                     /locus_tag="KJL99_002071"
     CDS             7758..8078
                     /locus_tag="KJL99_002071"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4436799.1"
                     /translation="MDEIRIRIAGNTAAPCFHVLKNKGYKVTMETHALSDDCLDCVYD
                     WYAEKNGQFFSATSPEELLGLVAMWEVRGNDWRANLNEINTYQKAIEESPFFDKNGNE
                     IESD"
     gene            complement(8144..8376)
                     /locus_tag="KJL99_002072"
                     /pseudo
     CDS             complement(8144..8376)
                     /locus_tag="KJL99_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000541009.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
     gene            complement(8486..9256)
                     /gene="yafV"
                     /locus_tag="KJL99_002073"
     CDS             complement(8486..9256)
                     /gene="yafV"
                     /locus_tag="KJL99_002073"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HBD4436800.1"
                     /translation="MPSLKITLLQQPLVWVDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHKATRIDAELSMVALREYREKFPAWRDADEFRLW"
     gene            9437..9883
                     /gene="ivy"
                     /locus_tag="KJL99_002074"
     CDS             9437..9883
                     /gene="ivy"
                     /locus_tag="KJL99_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HBD4436801.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     AIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(9926..12370)
                     /gene="fadE"
                     /locus_tag="KJL99_002075"
     CDS             complement(9926..12370)
                     /gene="fadE"
                     /locus_tag="KJL99_002075"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HBD4436802.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLTIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYSHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMALLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDNLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKMAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALTKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            12610..13188
                     /gene="lpcA"
                     /locus_tag="KJL99_002076"
     CDS             12610..13188
                     /gene="lpcA"
                     /locus_tag="KJL99_002076"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HBD4436803.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            13394..14161
                     /locus_tag="KJL99_002077"
     CDS             13394..14161
                     /locus_tag="KJL99_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HBD4436804.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(14132..14872)
                     /locus_tag="KJL99_002078"
     CDS             complement(14132..14872)
                     /locus_tag="KJL99_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HBD4436805.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVAN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            15256..15777
                     /locus_tag="KJL99_002079"
                     /pseudo
     CDS             15256..15777
                     /locus_tag="KJL99_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            15953..16450
                     /gene="rayT"
                     /locus_tag="KJL99_002080"
     CDS             15953..16450
                     /gene="rayT"
                     /locus_tag="KJL99_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HBD4436806.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSHLLTTQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPEKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDITDLSAG
                     ERIIL"
     gene            complement(16667..18379)
                     /locus_tag="KJL99_002081"
                     /pseudo
     CDS             complement(16667..18379)
                     /locus_tag="KJL99_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            18351..19136
                     /gene="lafU"
                     /locus_tag="KJL99_002082"
     CDS             18351..19136
                     /gene="lafU"
                     /locus_tag="KJL99_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HBD4436807.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLYNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            19207..20262
                     /gene="dinB"
                     /locus_tag="KJL99_002083"
     CDS             19207..20262
                     /gene="dinB"
                     /locus_tag="KJL99_002083"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HBD4436808.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            20314..20607
                     /gene="yafN"
                     /locus_tag="KJL99_002084"
     CDS             20314..20607
                     /gene="yafN"
                     /locus_tag="KJL99_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HBD4436809.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYLNDMTDDDFNDFKE"
     gene            20610..21008
                     /gene="yafO"
                     /locus_tag="KJL99_002085"
     CDS             20610..21008
                     /gene="yafO"
                     /locus_tag="KJL99_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HBD4436810.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            21018..21470
                     /locus_tag="KJL99_002086"
     CDS             21018..21470
                     /locus_tag="KJL99_002086"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBD4436811.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTVSQHYSLQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            21716..22759
                     /locus_tag="KJL99_002087"
     CDS             21716..22759
                     /locus_tag="KJL99_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HBD4436812.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALAFARFNRHLIALRIMQQVK
                     ATGSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSRLVQPVRSE
                     ETLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYK
                     SAESVVQCQGRPD"
     gene            22821..23435
                     /locus_tag="KJL99_002088"
                     /pseudo
     CDS             22821..23435
                     /locus_tag="KJL99_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(23492..24949)
                     /gene="pepD"
                     /locus_tag="KJL99_002089"
     CDS             complement(23492..24949)
                     /gene="pepD"
                     /locus_tag="KJL99_002089"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HBD4436813.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRQPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            25211..25669
                     /gene="gpt"
                     /locus_tag="KJL99_002090"
     CDS             25211..25669
                     /gene="gpt"
                     /locus_tag="KJL99_002090"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBD4436814.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            25761..27005
                     /gene="frsA"
                     /locus_tag="KJL99_002091"
     CDS             25761..27005
                     /gene="frsA"
                     /locus_tag="KJL99_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HBD4436815.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            27063..27464
                     /gene="crl"
                     /locus_tag="KJL99_002092"
     CDS             27063..27464
                     /gene="crl"
                     /locus_tag="KJL99_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HBD4436816.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(27503..28558)
                     /gene="phoE"
                     /locus_tag="KJL99_002093"
     CDS             complement(27503..28558)
                     /gene="phoE"
                     /locus_tag="KJL99_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HBD4436817.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDTVAVGMTYQF"
BASE COUNT         7084 a         7125 c         7608 g         6859 t
ORIGIN      
        1 acaaatattg atggcaatat cattatacat aaggatggta ggaggacgta tttattaaat
       61 caaaatactg gttcgattac ggctgtgata agatggaagt atgagtagta taaggataat
      121 accgttacag atgactggac tatatgggaa aaagctattt ttatgcgagc gatatattta
      181 tctgttcagc aggcatggaa tgggaaaata acttattctg tttccggtga atctgagttc
      241 gctaaaaaat ttcagggtaa agctttgcct tttgatgtaa gaataatttc tgcatctcag
      301 aatgaagact ggttggttat tgcaacaaag gttttgcctg gtgctgattt aagaacatat
      361 gttgatttta aaaacagtac ggttcatgtt gattcagcgg gcctggaaaa agtagcgaaa
      421 tgtattaact gtaataatac gttgcaagta aatatacctc atgaagccgg acatgtactt
      481 ggttacttag atgatgatta tgattcttcg tctccgtatg ttggagatat tagtgggttg
      541 atgaatgtgg gaatggagct ttgggaacga taccttaaaa acgcaactat aacgttgaat
      601 atcataatgc ctgaaactaa atttacttta ttgaatgtaa ccaaatgaaa tttttctgta
      661 ttatagtgat tttcctttca ataaattaca tagctatagt gaatgttttc aagtcttctg
      721 cagttgctca atccaccaaa tattatgaag aaaatgatat acatgatgga agtaatgaca
      781 tcttttttat gaagaaatat gggccgcctg ctcatgagtt cgtgaggcca cgatataaat
      841 ttgttggtga gtattatatc ggcttgaatc aaacttatcg cataacagat cctgaatttc
      901 aagatgttcc tattaaggaa atgttctgga atttgcaggg ggatttgaat ctgacatgtt
      961 ggtttcatta taaagacggg caatggagag tgattactta tattttttgg ccaccagggg
     1021 ctagatttta atccggtatt aaaggagcaa atatcatgag ttttgtatct acgggtaata
     1081 aaccagttgg aaacggtgga cccgtcatca ccacaccacc aatagctggc gaaagtggcg
     1141 gtatgagcac aggttcagct gtaaccgatg tatctggtgc cgccgaggag atggcagaac
     1201 aggctgcggc ggacctgttt ggtgcgctgc cggaaccgtc aggtctggtt aaagccgcag
     1261 tagctgctgc gcaggctgcc gctgcagcag cagggatctc ggatatggct ggtgcagttc
     1321 aggatgcagc ggcgtcgctt gcagccggtg ctccgggggc gcataacgtc acagtaagtg
     1381 gtagtgcggt gccgccgcag atgcttttgt ttgcaggcat gaatggttcg gaagggcttg
     1441 ggaatctgtt ttcttacaca gtacagctta aaacgctgga cgccctgaac ctggggtacg
     1501 tttcccccgc tgctaacctg ccgcttcagc tcatggtggg caaagacctg tgcgtcagta
     1561 tcgaactgga cggcggcgga aaacggtata tcagcgggct ggtgacggcg gcgagggtgg
     1621 tcggccatga gagccgttcg gtaacgtatg aattacgcat tgagccatgg ctgaagctgc
     1681 tgacccatac cagcgactac aaagcgttcc agaacaaaac cgtggtggat attctcgatg
     1741 aagtactgga tgaatattcc tttccggtgg aaaagcggct ggtggaaaat tatccacccc
     1801 gcgcctggca ggtgcagtat ggtgaaaccg acttcgattt catccagcgc ctgatgcagg
     1861 aatgggggat ttactggtgg tttgagcaca gcgagaacag ccacacgctg gtgctggtgg
     1921 atgccatcaa tggtcataaa gcgtgccctg actcgccgct ggtggagtgg caccaggaag
     1981 ggctgaaact ggataaggaa tttatccaca ccattacggc cagtgagagg ctgcgtactg
     2041 gaaagtgggt gatggacgat tttgatttta tgaagcctcg ttcgctgctt aaaagtaccg
     2101 tggccagtcc gcgtgatacc ggtcatgctg aatacgagca ttacgaatgg cctggtgatt
     2161 acttcactac gggcgaaggc gagatgctga cgcgcattcg tatggaagcg cagcgcagcc
     2221 caggcagccg ggcacatggg gccggtcata tccgtaccct catgaccggg tacaccttta
     2281 cgttgatgaa tcacccgacg gcagaaatta atcaggaata tctgctggta cagaccactc
     2341 tgtttttacg ggataatgcg cagcacagcg gtcaggatca gcattttacc tatgtcacca
     2401 cgtttgaact gcaccccacc agcgaagttt accgcccaca acgaaccctg agcaaacccc
     2461 acaccaaagg cccgcagagc gccatcgtca ccggcccggc ggggcaggaa atctggacgg
     2521 ataaatatgg ccgggtgaag gtgcagtttg gctgggaccg ttacggaaag aatgacgaaa
     2581 acagttcctg ctggatccgc gtcagctatc cgtgggcggg taaaggattc ggcatgatcc
     2641 agatcccgcg tattggccag gaagtactgg tggatttcaa aaacggcgat ccggatttgc
     2701 ccatcatcgt ggggcgcacc tataaccagg acaccatgcc gccgtggggg ctgccgggca
     2761 tggcgtcgca gagtgggata ttcagccact cattgcaggg agggccgact aacggaaata
     2821 tgctgcgctt cgatgataaa acgggtgcgg aagaggtgaa gttccatgcg gaaaaagacc
     2881 tcaataccac ggtaaaaaac aatgaaaccc acacggtcat ggtcgatcgc ataaaaacca
     2941 ttgtcaaaaa tgaaaccatc agcgtgggag aagaccgtaa tgccaccatc acaaaaaatg
     3001 atggtctttc cgtcaaactg gctcaaacca tcaatgtcgg aacgacatat cgtctggatg
     3061 ttggcgatca atttaccctt cgttgcggca atgcagcgct tgtattacac aaggatggct
     3121 cgattgagtt ttgcggaaaa caactgttac tgcacaccag cgatgttatg caactcattg
     3181 gcagtggcat tgatatgaat ccggatggcg gcacagccat gactgcggat gatattgccc
     3241 cgttacctac ccccgacgtt tctgaataaa aaatataaaa ggaatttttc tatgaccagc
     3301 ctttaccgta ttcaggaggg ctgctttgcc ctgcctgaaa cttttcttga tcgcaccgtc
     3361 aatatcttcg tcccatcggg caatgaaagg gcaactccca gtctgaatat tttccgcgat
     3421 acgctcaggc cagatgaaaa tctgaccacc tatattgacc gccagatagc cttgatgaaa
     3481 aaaaaaacct aggccagcac cgggtacttt cgcggggaac ggcactggcg ggaactggcg
     3541 aagctgccat gacaggtgaa cagattctcg caactcatcg ttctggaaaa acagaagtgt
     3601 accagcgtca ggccgggttt atcaccgggc cagcaaaggt actaatgttg acgctgacca
     3661 ctcagcgtcc ttttgatgat cacaccgacc agctatggac tgcatggttg actagttttc
     3721 agcctgctaa aagctaaaaa aaacagggag gttttgtcat gtatgaagcc gcacgggtgg
     3781 atgaccccat ctaccacacc agcgcccttg ccgggttttt gataggggcc atcataggta
     3841 tcgccattat tgccgtcgcc agctttgctt tttttacctg cggctttttg gccgggctga
     3901 ttttgagttt tctggctgat cagatagctt ccggagtcct gcaactgggt gaggctattg
     3961 ggcgctcgat acattccaca gtgggaaaaa ttctgaccgg ttctgataat gtcagcacca
     4021 acagtcgccc ggccgcccgg gccgtactga gtacggtgat atgcgaagac cattccccgg
     4081 aaatccgcat tgcccaaggt tccggcaaca tctacatcaa cagccagctg cccgtaagga
     4141 tgaccacact gagtgtgatg cagtgattga ggacggctcc cccaatgtct ttctcggcgg
     4201 cggtacgcag acggtactgg aaatcagccc ggaaatccct gactggctgc gtcaggtggt
     4261 ggatgtgctg tatgtggtcg ccggaatgct tggcgggctg gctggcgcct ggcgtcaggc
     4321 ggcaaagatg gggtcgaaat ttggtacaaa atgtgcggca aaatttattg gtggcgaaat
     4381 ggtcgggatg gccatctctg acacagtcat gggattgttc agcaatccgg tagatgtcac
     4441 cactgggcaa aaaattttgc tgccggagac ggattttacc ctgccaggac gcctgcctgt
     4501 cacctgttcg cgcttttacg ccagccacct ggaaactgaa ggattgctgg gcagaggatg
     4561 gcggctgaac tgggagataa acctgcgtga ggatgaaaca tatatcacct tcatcggtgt
     4621 acagggtcgg gaactgagtt atccgaaaga gatgctgata ccgggccacc agatattcga
     4681 cccggaagag cagttttacc tgagccgtct gcatgacgga tgctatgtgc tgcattacac
     4741 cgactgcagc tattacgtgt ttgatgaatt tgatgaccac ggcgtcgcgc cgttactttt
     4801 tatggagacg ccgtaccggc agcgtattgc tttcgggcgt gagaacggca ggctggtgcg
     4861 ggtggcctcc agcagcggac accatctgtt attgcaccgc acgatgacgc aagcagggga
     4921 acgcctgtcg catattgaac ttttgaaggg cgggcgtccc ggtaacctgg tggaatatcg
     4981 ttacgacgat aacgggcaac tgactggcgt ggtgaaccgg gctggtgtga cggtgcgcca
     5041 gtttgcctat gaaaatggcc tgatgacaga gcatcgcaac gcgacgggct ttacctgtac
     5101 ctaccactgg gaagagattg aaggtttccc gcgcgtggtg gaacacacga ccagcgacgg
     5161 cgaggattac cggttccact atgattttgc tggcgggcag acggtggtaa ccggcaggcc
     5221 ggagcagaag tggcagtggt ggtttgatga agagacgtat gtgactgcgc accggacgcc
     5281 gggcggcgga atgtaccgct tcacgtacaa cgaaaaccat tttccggtag cggtggaact
     5341 ccccggcgaa cgcagggtaa cgctggaata cgacaccctg agccgggtgg tgaaagagac
     5401 ggacccggca ggccgggtga cacagacgca gtggaacggc agttttgctg aaatcacgcg
     5461 ccgggcgctg gatgacgacc acgtatggaa ggcggattac aacgaacacg gccaggtgat
     5521 acgggagacg gacccggaag ggcgcgtcac gcggtacggg tatgatgatc aggggctgcc
     5581 ggagacggtc tgccaccccg gtaaacagca ggaccgttac acctggaacg cgctggggtt
     5641 actgagcagc caccgtcgca tcacgggcag tgtgcagtcc tggcagtata cgcagcgcgg
     5701 gatgctggcg cgccatacgg atgaagagaa acgggagacg cgctggcagt acaccccgga
     5761 agggctggtt gccagtctga gcaacggcaa cggggctcag taccggttca gctacgacgg
     5821 cgacggacgg ctgacgggag aacagcgccc ggacggcctg ataaggatgt ttgcgctgaa
     5881 tgcggacggg tttccggtca tcatcccgac gcagggcaca gagggcgggg tgcgcaatga
     5941 acagcaggag cgtgatgcgc tgggccgcct gctgcgcagt gacacgcagc acagtacacg
     6001 gaccttcagc tacaaccgtc ttgaccagat aacggaagtg acgctcaccc ccacggaaga
     6061 aggggaacgg ctgcaccaca tgcaggcgga cacggtgcgt tttgcgtatg accgcagcgg
     6121 gtggctgacg gcagagcaca gcgtgcacgg cagtatcaaa taccggcgcg atgcgctggg
     6181 caatcccacc gatatcacgt tgccggacgg gcagcatctc agtcatcttt actacggcag
     6241 cgggcatctg ctccagacgg cactggacgg tatcacggtc agtgaatatg agcgtgacag
     6301 cctgcaccgt caggtgatac gcacgcaggg gaaactggcg acgttcagcg gctacaacgc
     6361 cgacaaccgg ttaagctggc agcgcagcct gccaggtggc agccagaatc cacagcaggc
     6421 ggtactcccc cggcgcagaa cgacagcgtg acggcgcggg actatatctg gaacgcagac
     6481 ggtgaagtcg gcggcatcaa tgacaaactg cgcggttgcc tggtgttcag ctatgaccgc
     6541 agcggctggc tgaccagccg cacggggcag atgtatgacc atgaccatta ttattacgac
     6601 aaagcgggca acctgctgac ggatgagtat cagggagcgg tgatggataa ccgcctgccg
     6661 ggttacgggc gtgaccgcta ccgctataac gaatggggcg aactgacaga gcgcagggac
     6721 cagcagctgg agtggaacgc gcagggccag ttaacgcggg tcatcagcag caacagcgag
     6781 acgcggtatc agtacgatgc gctgggcagg cgcatcagca aggcgaccag caatctccat
     6841 accgaccggg gagaacgaag ccggacaacg tttgtgtggg aagggttccg gctgttacag
     6901 gagacaacgt ggcagggaaa gcggacatac ctgtacgatg cggaacagcc gtacacgccg
     6961 gtggctgcca tcacggggcg cggagagagc cagaaaatct ggtattacca cacggacctg
     7021 acgggcacgg tacacgaggt aaccgcgccg gacgggacgc tggtgtgggc gggctaccag
     7081 gcgggatttg gtgaaaaccg gggcgacatc agcaacagcg gggcgtactt tgagcagccg
     7141 cagcggctgc cggggcagta ctttgacgaa gaaacagggc tgcattacaa tctgttcagg
     7201 tattatgcgc cggagtgcgg gaggttcgta agccaggacc cgataggatt aaacggtggg
     7261 ttgaacttat atgcttatgc gcctaatccg ttaggttgga tcgatccgct aggactgaat
     7321 tctttaggaa tagattcaaa taatatattt agaggagatg gtaactataa aggtggcgga
     7381 attggacagc caaagagtgg cacaatatca gcacaggaca ttattgatca tctctaagaa
     7441 aaaggtgatg ggcgtttaac atcttttagt actaaaaaaa catctagttc caactctcgt
     7501 ggtgcagatt ttttctcaaa aaaccatgtc ttcaaagtgg cagtttctga tctcgatgat
     7561 ttagttaaaa ataatcagat cactgtttta acacaagaaa aagtaaaagc aatatttggt
     7621 ttagaatcaa gcaagaaaat tagaaaagaa cttggtaata ttcttcagaa tatggctagg
     7681 aataatgagg tgttagttca tgggcaaatt cttggaaata aagttagccg atgtaattag
     7741 ttagtggaga ggaagttgtg gacgaaatta gaataagaat tgctggtaat acggctgcgc
     7801 cttgtttcca tgtgctaaaa aataagggat ataaagtaac aatggaaact catgcattat
     7861 ctgacgattg tttagattgt gtatatgatt ggtatgcgga aaaaaatgga caatttttta
     7921 gtgcaacgag tccagaagag ttacttggat tagttgcaat gtgggaagtt cgtggtaatg
     7981 actggagagc taatttgaat gagataaata cttatcaaaa agccatagaa gaatccccgt
     8041 tttttgataa aaacggcaat gaaatagagt ctgactaaat tttattgata tagcaacgat
     8101 aataaccgga aatttcttcc agatctttcc ggctttttat tatttaactt tcgttcagca
     8161 cactaaacat cccgtctcgc atccccttaa ctggctgttt aacctgataa agttccctgc
     8221 gcagttcctg tacttcgttg ttatcccaag caggatcaaa catccctccg agatcttcac
     8281 ggtaacgccc gtgccgatct caaatcccgc ttttctcagc catttggcgc tgattttgat
     8341 caccggcgtg tcgctgcggt tgggcagata acccacggta cagcgacgct gtgttttgga
     8401 aatggtgact tctggcgtaa tattgtgttt aagttagggg ctttcgtaat ctagcccagc
     8461 ccccggccct aaacacaacc cccactcacc acaacctaaa ctcatccgca tcccgccatg
     8521 ccggaaactt ttctctatat tcccgcaatg ccaccatcga cagctccgca tcaatacgcg
     8581 ttgccttatg cgcgtcggcg gtagcgataa tctcgccttg cggattaatc acccggctgt
     8641 caccgcgata atggcagccg ttgccatcgc tgccgacgcg attgcatccc gccacatacg
     8701 cctgattctc aatcgcgcgg gccgtcagca atgcctgcca gtgcagagag cgcggagctg
     8761 gccagttggc gacgtacagg gcgaggtcat aatcgttgag attgcgcgac cacactggaa
     8821 aacgtaagtc gtagcacacc agcggcaaaa tacgccagcc gcgccattcc acaatcactc
     8881 gcgcattgcc cgctttataa tgcagatgct catctgccat gcggaacaga tgacgcttat
     8941 cataaaaatg taccgtgccg cccggctcaa ccagcaaaaa gcggttaacc gaaccagact
     9001 ccgtttgtaa tgcaacactg cctgcaatca gcgcattgca ctgctgcgcc ttagctgtca
     9061 tccagttcac tacgtcatct tgtgcaagcg acgaagctgc cgcttccatg gcaaagccgc
     9121 tggtaaacat ctccggtaga acgatcacat cgcgcccggt aataccttcc agttgacgat
     9181 caaaatggcg caggttggca ggaccatcca cccacaccaa cggttgctgc aaaagcgtaa
     9241 tcttcaaact aggcacggtg tacaactcct ttatgcgaag ggttttataa ctttaacacc
     9301 ttatcaggca gttgccttag cgcagaataa attgataaca aatgctgata ttggaaatat
     9361 ctgatttgca aattatcgtg ttatcgccag gctttaggag gttaataaca tgggcaggat
     9421 aagctcggga ggaatgatgt ttaaggcaat aacgacagtc gccgcactgg tcatcgccac
     9481 cagtgcaatg gcgcaggatg atttaaccat tagcagcctt gcaaagggcg aaaccaccaa
     9541 agctgcgttt aatcagatgg tgcaagggca taagctacct gcatgggtga tgaaaggcgg
     9601 tacttatact cccgcacaaa ccgtaacgtt gggagatgag acgtatcagg tgatgagcgc
     9661 gtgcaaaccg catgactgtg gctcgcaacg tatcgctgtg atgtggtccg agaaatctaa
     9721 tcagatgacg gggctgttct cggctattga tgagaaaacg tcgcaagaga aactcacctg
     9781 gctgaatgtg aacgatgcgc tttcgattga tggtaaaacg gtgctgttcg cggcgttgac
     9841 cggcagcctg gaaaaccatc cggatggctt taattttaaa taattagcgg ataaagaaac
     9901 ggagcctttc ggctccgtta ttcaattacg cggcttcaac tttccgcact ttctccggca
     9961 actttaccgg cttcgtcgcc agctcttccg gatcaaagtc atcaacgtta atactgcgca
    10021 gacggctttc ttccgctttc accagaatag cggcttcatc tttatcaatc agccccttcg
    10081 tcagcgcgtt gtgcgccagt tcatccagac gggtaaatgg cagattttta cccagctctt
    10141 tacagatccg ctgatgaatc gggtcggcgg caatcacatc caccaacgcc tcttccagta
    10201 aaccaaccgg gttatgctcg cttggcgtca ggtactgacc gcgaccaata cgtgaacgag
    10261 tggcgttcgg cacttgtaaa atcttcgcca ttttatgatc cagcttgtca gacggtgcca
    10321 gataatgacg tccggtcggg aagatcacca cattcagcag cccggcaacc acgcggttcg
    10381 ggaagttttg cagtaaattg tccattgcct gttctgcctg atacagcgca tcctgtacgc
    10441 cccagtgcac cagcggcaga tcggcttcat tacggccttc gtcgtcataa cgcttcagaa
    10501 cggcagaggc gaggtaaagc tggcttaaaa tatcccccag acgtgccgag atacgctcac
    10561 gacgtttcag gctgccgccc agcagtgcca tcgagacatc agaaagcagg gcgaggttgg
    10621 cgctcaggcg gttcaggtgc tgatagtagc gtttagtggc atcgccggtt ggcgtgctgc
    10681 tggttaaacc gcgcgtcagg cccagccaga agctgcgaac tttgttgcta ccgacgtgac
    10741 cgatatgttt gaacaacagt ttatcgaacg cgttgacgtc attgttcttc gccgcttcca
    10801 tctcttccag cacgtacgga tggcaacgaa tcgctccttg tccgaagatc atcatgctgc
    10861 gggtcagaat gttagcccct tcaacggtga tggcaatcgg tgcgccctgg taagcacgcg
    10921 ccaggaagtt gctttgcccg agcataatgc ctttaccgcc ggtaatatcc atcgcatcaa
    10981 taatcgactg ctgcccgcgg tgggtacagt gatacttaac gatagccgac agcacggcag
    11041 gtttttcgcc gagcataatg ccgtaggtaa tcagcgatgc cgcagcatcc attacataag
    11101 cgttcccggc aatacgcgcc agcggctctt caatcccttc catcttaccg atagagattt
    11161 tgaactgacg gcgaatgtga gaatacgcgc ccgttgccag cgctaccgat ttcacgccgc
    11221 cggttgagtt ggaaggcagg gtgatgccgc ggcctaccga gaggcactcc accagcatcc
    11281 gccagccttg cccggccatt ttcggcccgc cgatgatgta atcgatcggc acgaagacat
    11341 ctttaccgcg cgtcggtccg ttctggaacg gtacgttcag cgggaagtgg cgacgaccaa
    11401 tttccacgcc cggcgtggtg gttgggatca gcgcacaggt aatgcctaaa tcttctgcac
    11461 cgccgagtaa tttttccggg tcggagagtt taaacgccag cccaagcacg gtcgcaatcg
    11521 gtgccagcgt aatgtagcgt ttgttccagg tcagacgcat ccccagcacc tgctggccct
    11581 gccattcgcc catgcagaca atcccggtgt ccggaatcgc gcccgcatcg gaacccgctt
    11641 ccgggctggt cagtgcaaag caggggatct cctgaccacg cgccagacgc ggcagatagt
    11701 gatctttctg ctcgtcagtg ccgtaatgtt gcaacagttc gcccgggcct aatgagtttg
    11761 gcacgccgac ggtaatcgcc aggatcccgc tcacgccaga gagtttttgc agcacacgag
    11821 actgggcata agctgagaac tccagtccgc cgtactcttt tttgataatc atcgcgaaga
    11881 agcgatgctc tttaaggtac gcccacaact ccggtggcag atccgccagc tcatgagtaa
    11941 tctggaaatc attcgccatc cggcaggctt cttctaccgg gccgtcgaga aacgcttgct
    12001 cttcagcggt caggcgcggc tgcggatagt tatgcagctt tttccagtcc ggcttgccct
    12061 ggaacaagtc gccctcccac caggtggtgc ccgcatcaat cgcttctttc tcagtgcgcg
    12121 acatcggcgg catcacctta cggaaaccgc gaaataccgg cgcggaaatc atcgacttac
    12181 gcataggcgc aaagttaaat ggcacgagga taatggtcag aggcaccagt acccacgccg
    12241 accacagacc agcaacgccg agggcggctg tccaggcgag caaaatcaga ctgctgataa
    12301 ataagctcac gcggtgatag aacaacgcgc cgagcaggac aaccgtagcg agaatactca
    12361 aaatcatcat aacgaaaagc cccttacttg taggaggtct gaccacttgt gatgatatgg
    12421 ttgtagtgga tgtaaaaaca tttagcaatg tgtttacaat ataattacaa caaagctcac
    12481 attgttgctg tttttatccg cacttcaggt caaaaagtcc tggtcatagc acctgcccgt
    12541 acttctcgct tttggcggta tccggtacac tgcattttgt ctattacatt tatgctgaag
    12601 gatatcctca tgtaccagga tcttattcgt aacgaactga acgaagcggc ggaaacgctg
    12661 gctaactttt taaaagatga cgccaatatt cacgccattc agcgcgcggc ggtcctgtta
    12721 gcagacagct ttaaagccgg tggcaaagtg ctttcctgcg gcaacggcgg ttcccattgc
    12781 gacgctatgc actttgccga agagttgacc ggtcgctacc gtgaaaaccg tccgggctac
    12841 ccggcgattg ctatttctga cgttagtcat atttcctgcg tcggtaatga tttcggtttc
    12901 aatgatattt tctcccgcta cgttgaagcg gtaggtcgcg aaggcgatgt actgctgggg
    12961 atctccacct ccggtaactc tgcaaacgtg atcaaagcga tcgcagcggc gcgtgagaag
    13021 gggatgaaag tgatcaccct gaccggtaaa gacggcggta aaatggctgg cacggcggat
    13081 atcgaaattc gcgttccgca ctttggttat gccgaccgca ttcaggagat tcacattaaa
    13141 gtgatccata tcctgatcca gttaattgaa aaagagatgg ttaagtaagt ctggcgtagg
    13201 ccggataagg cgtttacgcc gcatccggca cttgtgcgct gatgcctgat gcgatgctga
    13261 cgcgtcttat catgcctaca aatctgtacc cgaaccgtag gccgaataat tcgtttacgc
    13321 cgcatccgac ctgaaaattc ttaaatcaat cttcgccggg gtccatgcgc tcccgctgtt
    13381 gtggaggtta cccatgtgcg aattgctcgg gatgagcgcc aacgtcccta ccgatatctg
    13441 ctttagtttc accggacttg tacagcgtgg tggtggaacc gggccacata aagatggctg
    13501 gggcattacc ttttacgaag gtaaaggctg tcgcacattt aaagatccac aacccagctt
    13561 taattccccc atcgccaaac ttgtccagga ctacccgata aaatcctgtt cggtggtggc
    13621 tcatattcgc caggctaatc ggggcgaggt ggcgctggaa aatactcacc catttacccg
    13681 cgagttatgg gggcgtaact ggacttatgc ccataacgga caactgacgg gctacaaatc
    13741 actggaaacc ggcaacttcc gcccggtcgg tgaaaccgac agcgaaaaag ccttttgctg
    13801 gctgctgcat aaattaacgc agcgttaccc gcgcacgccg ggcaacatgg cggcagtgtt
    13861 taaatatatc gcctcactgg cggatgaact gcgggcgaag ggcgttttca acatgctgct
    13921 ttcggacggg cgctatgtaa tggcgtattg ttcgactaat ttacactgga tcacccgccg
    13981 cgcgccgttt ggcgtggcaa cgttgctgga tcaggatgtg gaaatcgact tcagctcgca
    14041 gaccacaccg aatgatgtgg tcacggtgat tgcgacacag ccgctgacgg gcaatgaaac
    14101 ctggcaaaaa attatgccgg gcgaatggcg cttattttgc ctcggggagc gtgtagtttg
    14161 atgccagttg tggctgcacc acttcgtggc ttaacggctt gctgaccacg taacggccat
    14221 tggccacaga aacggtcggt ggcttacggg tttgctcaaa gtagtcgtag cccggcttca
    14281 gctgctccca gaagtcctta aagttggaat atttatggcg cttcatattg gcgtcggtca
    14341 tgcggaatgg gtaaatactc acctgcacgc tcggctgacc aaacaccagc gcaccagtaa
    14401 cgaactggaa gatctcatca ataccctgat tggtcattgc gtagcagccg atggatacac
    14461 aatcgccgtg gatcatcagg tatttccctt cataaccatg cgcacggtca taagcattgg
    14521 ggaatccaat attaatcgct ttgtagtaac ggctgtctgg ttttaactga ttacgctgga
    14581 cgctataaaa cccttccggg cttttgaaat cgccctgacg ctgttttggc cctaagccgc
    14641 cggaatattt acagatttta taactgtcga gcagttgata ttgctcgccc attttgacgt
    14701 aaagatcgag cgtacgttct tccttgaaga tctggatgta gacaggggat cccatcaact
    14761 gctgcttata ctctttgctc actggcgtgg tcgagctact gctgcccagc aaaccggcaa
    14821 acgaaacgca cgggatcaac aacatcgcaa gaattaatgc gattttacgc atactgctta
    14881 ttccttgata aaacggttac acacgccagg acggcaaaat ggatcccaaa tcggaatagt
    14941 ctggatttgg aaggctcaca ttatcaccaa aagagtttta cgcaagcctg tcggcgctgg
    15001 gtttacaaat ttagcacagg ggcagttttc aatagtccga aaagcgcatg tatcatttat
    15061 ttcctaatat tatgaaatct caatagtaat atgcaaataa gcaattaata atcccgttta
    15121 ctcctccggt cttttaaaat tctcaccttc attcagccgg tttatgtctt tcatgtcttc
    15181 gttcttgctc ggcagatttt tgcatccagg cgtgttttcg ttatgcgttt tgctcccttt
    15241 actcgccagc gccaccacaa ccgggagcag ataaaacagc atccagaatg gtttcctgcg
    15301 ccgctcaagg cgagtgacag acgctggcag gcgctggcgg aaaacaacca ctttttaagc
    15361 agcgatcacc tgcataacat caccgaagtg gcgattcacc gcctggagca gcagcttggc
    15421 aagccttacg tctggggcgg cacgcggccc gatcaaggct tcgattgtag tgggttagtt
    15481 ttttatgcct acaacaagat ccttgaggcg aaactcccgc gcacggccaa tgagatgtac
    15541 cactaccgtc gggcaacgat tgtggcgaat aacgacctgc gccggggaga tttgctattt
    15601 ttccatatcc acagccgcga gattgccgac catatggggg tgtatctggg cgacggtcag
    15661 tttattgagt cactgcgcac cggggaaacc attcgggtaa gccggttagc cgaacctttc
    15721 tggcaggacc attttttagg cgcgcgcagg attttgacgg aagagatgat tttgtaggcc
    15781 ggataaggcg tttacgccgc atccggcggt tgtgcacgga tgcctgatgc gacgctgacg
    15841 cgtcttatca tgcctacaaa tctgtgcgtt tttgcgagcc gctttcccga tataacacag
    15901 cgtcattgtc ggcctttcct tttcctctta ccgttgagaa aaaggagtcg ctatgtcgga
    15961 atatcgtcgt tattacatca aggggggaac atggtttttc acggtaaatt tacgcaatcg
    16021 tcgaagccat cttctgacca cccagttcca gacgctccgt aacgccatta ttaacgttaa
    16081 gcgagacaga ccttttgaaa ttaacgcctg ggtcgttttg ccggagcata tgcattgcat
    16141 atggacatta cccgaaagcg atgatgattt ttcctctcgc tggcgggaga ttaaaaagca
    16201 gttcacccat gcttgcggat tgaaaaatat ctggcaaccg cgtttttggg agcatgccat
    16261 tcgcaataca aaagattatc gccatcatgt tgattatatt tatataaatc cagagaaaca
    16321 tggctgggta aagcaggtga gtgattggcc attctcaacg ttccatcgcg atgtcgcgcg
    16381 agggttatat cccatcgatt gggcgggaga cataacggat ttaagtgccg gggagcgtat
    16441 cattttataa ctgtgcgctg gtgccggatg cgcttacgct tatccggcct acaaatcgtg
    16501 ctttaacggt aggccggata aggcgttcac gccgcatccg gcagttatgc gcagatgcct
    16561 gatgcgacgc tggcgcgtct tatcatgcct aaaaacttgt gcctgaacgg taggccggat
    16621 aaggcgttta cgccgcatcc ggcaattgtg caccgatgcc ggattcttac atcaacgccc
    16681 ccataatctt caactccagc tcatccggca cttcgttata cgacagcaca tgcagccccg
    16741 gcgcaaacaa ccttgcataa cgcggcagca aagggcgtag ctgcggcggc accagcagca
    16801 ccggatcttt ccccgccgct ttcatctgct ctttcacctg tggcatcgtg ctctggaact
    16861 ggttgagcat attcgggtca accggcacgc tgtcgagcat cactttgccg gcctgttgcg
    16921 cctgattcac tacgttggtc agcagatttt ccagctcatt attcagcgta tacaccgtca
    16981 gctcctgctt gcgaacgaac ggatgagtaa tgctgcgccg cagcgccagg cgcacatcgg
    17041 ccgccagcag aatatgatct ttagtcaccg tgctactggc gaccagcacg gtggcgatgg
    17101 tgacgatatc gcgcagggaa acgccttcgg tcagcagcgc ccggtacact ttcagcaatt
    17161 ggctgtaatt gagcgccgcg cttaaatcct ccgccaggcg cggtgccatc gacgacaaac
    17221 ggttatgcaa ctgcgtgatg tcatcatagt taaacaaatc aggaatatag ctgcgcacaa
    17281 tcttgttcac atgggtggca atcacactgg cgctgtcgat cacctgatac cccatattca
    17341 gagccttcgc tttctgcgca ggctggatcc aggtgaccgg tatcccgtac gccgggtcgt
    17401 tccccagcac gccgtcaatc tcgccgtagg tttcgctgga gggcagcgcc atcagtttat
    17461 ccgccggaat atccgcttca tcagcctcaa tgccgttgat gaaaatagcg tactggctgg
    17521 gcttgaggcg gaagttttcc cgaatgcgga tctccggcag cagcacgccg ttaccgtcgg
    17581 aaatcacctg ccgaacgccg cgaatccgct gggtgagcgg gttgccctgc gctttgtcca
    17641 ccagcgccac cagcttgtag ccgaggctta agctgatggg ctcgatcagc ggaatagttt
    17701 cccaactgac ctgttgttcg ctggtttcag tgatggtgcg ggtcagcgtt tcgaggcttt
    17761 tctcttccgc ctccgccgcc tgcgggcgtt tgctcatccg ccagccggta aagccaagca
    17821 gggcgctgaa cagcaaaaac ggcagatgcg gcatccccgg caccaccgcc agcacgaaca
    17881 tgatcccggt ggcggtgtag agcaccgacg ggcttgccag caactggtgg cgcacgtcat
    17941 gggcgatatc gccgttgtcg ctgacgcggg tgacgataat cgccgccgcg gtggagagca
    18001 gcagggaagg gatctgcgcc accaggccgt cgccgatggt catcagcacg tattgctgga
    18061 aggcggcgtc ggcgctcagg ttgtatttga agatgccgat acagacgccg ccgctcaggt
    18121 tgatcgccag aatcatcatc ccggcgatgg cgtctccgcg tacaaacttc gatgccccgt
    18181 ccatcgcgcc gtagaaatcg gcctcgctgg caacatcttt acgccgggtt tgcgcctgcg
    18241 cctggttgat caatccggcg ttaagatcgg cgtcaatcgc catctgtttg ccgggcatcg
    18301 cgtccagggt aaagcgggca gaaacctcgg aaatacgctc ggcctctttg gtgacgacga
    18361 taaagttgat tgtcaacagc gtcagtaaat ccgagcgtga aagcattatc gccgcgctgc
    18421 acgggcagtc gatttttaac ggtggtgggc tgtcgccgtt aaataaaatc agcccgtctc
    18481 atccgccaaa acctgccacc gtggcggtgc cggaagagac ggagaaaaaa gcgcgtgatg
    18541 tgaatgagaa aacggcgctg ctgaagaaga aatccgccac cgagcttggc gagttggcaa
    18601 ccagtatcaa caccattgcc cgtgatgcgc atatggaagc gaatctggag atggagattg
    18661 ttccccaggg attacgcgtg ctgattaaag acgaccagaa tcgcaatatg ttcgaacgcg
    18721 gcagcgcgca gattatgccg ttctttaaaa ctttgctggt ggagctcgcg ccagtgttcg
    18781 actcgctcta taacaaaatt attattaccg ggcataccga tgcgatggcc tacaaaaaca
    18841 atatctacaa caactggaac ctttcgggcg atcgcgcgct ttcagctcgt cgggtgctgg
    18901 aagaggccgg aatgccggaa gataaagtga tgcaggtaag cgcaatggcg gaccagatgc
    18961 tgctggacgc gaagaatccg caaagcgcgg gcaatcggcg tattgagatt atggtgctga
    19021 ccaaaagtgc gtccgatacg ctgtatcaat actttggtca gcatggggat aaagtggtgc
    19081 agccgctggt gcaaaagctg gataagcagc aggtgctttc gcagcgaatg cgctaaatgc
    19141 tgaatcttta cgcatttctc aaaacctgaa atcactgtat actttaccag tgttgagagg
    19201 tgagcaatgc gtaaaatcat tcatgtggat atggactgct ttttcgcagc ggtggagatg
    19261 cgcgacaatc ccgctctgcg cgatatccct attgctattg gcggcagccg cgaacgtcgg
    19321 ggggtgatca gcaccgccaa ttatcccgcg cgtaaatttg gcgtacgtag cgctatgcct
    19381 actgggatgg cgctcaaatt atgcccacat ctcaccttgc ttccggggcg ctttgacgcc
    19441 tacaaagaag cctcaaatca tatccgtgaa atcttctcgc gctacacctc gcgcattgaa
    19501 ccgttgtcac tggatgaggc ttatctcgat gtcaccgata gcgtccattg ccacggttct
    19561 gcgaccctca tcgcccagga aatccgccag acgatcttca acgagctgca actgacggca
    19621 tctgcgggcg tggcaccagt aaagtttctc gccaaaatcg cctccgacat gaataaaccc
    19681 aacggtcagt ttgtgattac gccggcagaa gttccggcat ttttacaaac cttaccactg
    19741 gaaaaaatcc ccggcgtcgg caaagtctcg gcggcaaaac tggaagcgat ggggctgcgg
    19801 acctgcggtg atgtacaaaa gtgtgatctg gtgattctgc ttaaacgctt tggcaaattt
    19861 ggccgcattt tgtgggagcg tagtcagggg attgacgagc gcgacgttaa cagcgaacgg
    19921 ttacgaaaat ccgtcggcgt ggaacgcacg atggcggaag atattcatca ctggtctgaa
    19981 tgtgaagcga ttatcgagcg gctgtatccg gaacttgaac gccgtctggc aaaggtaaaa
    20041 cctgatttac tgattgctcg ccagggggtg aaattaaagt tcgacgattt tcagcagacc
    20101 acccaggagc acgtctggcc gcggctgaat aaagctgact taatcgccac cgcgcgtaaa
    20161 acctgggatg aacgccgcgg cgggcgcggt gtgcgtctgg tggggctgca tgtgacgttg
    20221 cttgacccgc aaatggaaag acaactggtg ctgggattat gatgtatact attatgtata
    20281 ttctggtgtg cattattatg agggtatcac tgtatgcatc gaattctcgc tgaaaaatcg
    20341 gtcaatatca ctgagttacg taaaaaccca gctaaatact ttattgatca accagttgcg
    20401 gttctttcta ataatcgccc cgcaggatat ctcttaagtg ccagcgcatt cgaagcgtta
    20461 atggacatgc ttgctgaaca agaggagaaa aagcccataa aggcgcgctt tcgtccaagt
    20521 gctgcaagat tagaggaaat tacacgccgc gctaaacaat atcttaatga tatgacggat
    20581 gatgatttca atgactttaa ggaataagga tgcgggtatt caaaacaaaa cttattcgcc
    20641 tgcaacttac agcagaggaa cttgatgcgt taacggcgga ttttatttcc tataagcgtg
    20701 acggtgtttt gccagatata tttggtcgcg atgcactcta cgacgactcg tttacctggc
    20761 cattaatcaa atttgagcga gttgctcata ttcatctggc aaatgtgaat aatccatttc
    20821 cgccacagtt gcgccaattc agcagaacga atgacgaagc gcatttggta tattgtcagg
    20881 gtgcgtttga tgagcaagca tggttgctca ttgccattct gaagcctgaa cctcataaac
    20941 tggctcgaga taacaaccaa atgcataaaa ttggaaaaat ggcagaagcg tttcgcatgc
    21001 gtttttgaat ttatattatg aataacatac aaataagaga ttatcagcct ggcgattttc
    21061 agcaactatg cgctattttc attagagcgg ttacgatgac cgtcagtcag cattattcac
    21121 tacaacaaat tgccgcctgg gcgcagattg acgaatctcg ctggaaggag aaactcgcga
    21181 aatcacaagt gcgggttgca gttattaatg caaaactggt cggctttatt acctgcgttg
    21241 aacactatat cgatatgtta tttgttgacc ctgaatacac ccgccgtggg gttgccagcg
    21301 ctttgttaaa accttttatt aagtctgaat ccgaacttac ggtggacgca agcataaccg
    21361 caaaaccctt ttttgaacgt tatggttttc agacagttaa gcagcagcgc gttgaatgcc
    21421 ggggagcgtg gtttattaat ttttatatgc gttataaacc gcaacattaa atccagcttg
    21481 caatgaaaat aacgcccgcc tagtatgtgc gcgttttctt attccacaaa ctgcaaggag
    21541 gtaaatcatg acaaatcctt tatcaatgac tctttgcaga cctttccagg attaattctt
    21601 tttttcttgc cctggattcg tctgccattt cctgattttt taattgttac tctcaataat
    21661 tcaagtcgca gcacatgcaa cttgaagtat gacgagtcta tttacggaat ggtatatggg
    21721 caattatatt cgtcccttat ccgatgcggt atttaccatc gcatctgatg acctgtggat
    21781 cgagagttta gcgatccaac aattacacac cacggcaaat ttacccaaca tgcagcgcgt
    21841 agtggggatg ccagatttac accccggacg cggctacccg attggcgcag cgttcttctc
    21901 cgcaggccgt ttttacccag cactggtcgg caatgatatc ggctgtggca tggcgctatg
    21961 gcaaacagat attctcgctc gcaaatacaa cgccgataag tttgaaaagc gattatcagc
    22021 tttggatgac gttgctgaag aaagctggct ggaggaaaac ctgccgtcag catttgcaca
    22081 gcatccgtgg cgcagctcgc ttggctctat cggtggtggc aatcatttcg ctgaactgca
    22141 acaagttgat caaatcatca acgctgaact gtttgcactg gcaggtctgg atgcgcagca
    22201 tctgcaactg ctggttcata gcggctcgag ggggctaggg cagtctattt tacagcggca
    22261 tattgcctcg ttttcgcatc atggtttgcc tgaaggcagt gacgacgcgc tggcgtttgc
    22321 gcgttttaat cgccacctga tcgctttgcg cataatgcaa caggttaagg ccaccggaag
    22381 tccggttctg gatgtggcgc ataactttgt tagcgcgtgt cgaatcggtg atcaacaagg
    22441 ggtcttacat cgtaaaggtg ccacgcctga tgactgtggt ctggtggtta ttcccggttc
    22501 acgcggtgat tactccaggc tggttcagcc cgtcaggagt gaggaaacat tgcattcgct
    22561 ggcgcatggg gctgggcgta aatgggggcg caccgagtgt aaagggcgtc tggcagcgaa
    22621 atacacagcg acgcaactct cgcgtactga acttggcagt cgggtaattt gtcgcgataa
    22681 acaactcatc ttcgaagaag cgccacaagc ctataaatcg gctgaaagtg tggtgcaatg
    22741 tcagggcagg ccggattaat tattcctgtc gcgcggctgc gtccggtgct aacgctcaaa
    22801 aacagtggag ggaaaaaagg atgatcttgc tataactctc ctcagcgcag gggccggaag
    22861 aatgttgtct cgcagtgaaa aaggcgctgg acaggctaat taaagaagct gcccgacaag
    22921 acgtcgcggt aacggtgctg gaaacagaaa caggccgcta ctctgacacg ctgcgttcgg
    22981 cgctgatttc tctggatggc gacaacgcat gggcgttaag cgaaagctgg tgtggcacta
    23041 ttcagtggat ttgcccgagc ccgtatcgcc cacatcacgg gcgtaaaaac tggtttctgg
    23101 gcattgggcg ttttaccgct gatgagcagg agcaatccga tgcgatccgg tatgagacgc
    23161 tgcgttcgtc ggggccgggc ggtcaacatg tcaataaaac cgactcggcg gtacgcgcca
    23221 cgcatttggc atccggtatt agcgtgaagg ttcagtcaga gcgcagccag catgctaaca
    23281 aacggctggc gcgattgctg attgcctgga agctggatca gcagcaatag gaaaatagcg
    23341 cggtgctgaa atcacagcgg cgaatgttcc atcaccagat tgaacgtggc aacccgcgac
    23401 gaacgtttac agggatggct tttatcgaag gataatgaag gatgaaactg ccggaaggcg
    23461 attaaacgcc atccggcagc aaatcaaata attacttcgc cggaatttct ttcagcagtt
    23521 cagtcagcag tgtccagtaa tgacctacgc tttcgatgtg aacttgctca tccggagagt
    23581 gtggaccggt gatagttggc ccgatagaaa ccatgtccat ttccggatac ggttttttga
    23641 acagaccaca ttccaggccc gcgtggataa tctggatgtt cggcgtcttg ttgaacaggc
    23701 gctgataggt ttcacgtacc agatgcatca ccggagaatt agcgtccggc tgccagccag
    23761 gatatgcgcc tttcgcttcg gttttcgcgc cagccagttt acccagcgaa tccagcatgc
    23821 tcaccacgta gtctttaccg ctgtcgatta gtgaacggat caggcagtga atttctacgt
    23881 tattgtcagt catggtcacc acaccgacgt tcagggaggt ttcgaccaca ccttttgcca
    23941 cgtcggaatt gcggatcaca ccgttcgggg tggcgttcag cagacgaata aaggtatcgc
    24001 gagatttcgc aatcagggca gctttatcgt tcgctacaga gtccagcaac aaggccagat
    24061 ttttctcttt ctctgccagc tcgtttttca ggatctcctg ataggtattc accagagatt
    24121 tcagtgcgtc gactttatca gctgcgacag caatggtcgc aaaggcttca cgcgggatgg
    24181 cgttacgcag tgtgccgccg ttgaaatcga taaggcgcag gtccagttct tctgcatgac
    24241 ccgccaggaa gcgcaccagc agtttgttgg cattacccag gccaacgtgg atttcaccgc
    24301 cggagtgacc gcctttcaga ccttttaagg ttaacttgaa ggtttcaaaa ccagctggaa
    24361 ccgcttcacg atctaaatgc aggttggagg tgaagtcgat accccccgca caacccatgt
    24421 agatttcacc ttcttcttcg gagtcggtgt taatcagaat atcagcctgc aaccagttgc
    24481 tctgtaagcc gaacgcaccg tccataccgg cttcttcggt catggtcagc agcacttcca
    24541 gcgggccgtg aaccacgttt tcgtcagcca gaaccgccag tgcagaggcc ataccaatgc
    24601 cgttatccgc acccagcgtg gtgccgcgcg ctttaaccca ttcgccatca atataaggct
    24661 ggataggatc tttcgtgaag tcatgcacgg tgtcgttatt tttctgcggc accatatcga
    24721 ggtgggcctg taagacgacc ggtttacgat tttccatacc tgcggtagca ggttgacgaa
    24781 tcaggatatt acctacctga tcgcgttcga catggaaacc tttctctttt gcccaaccaa
    24841 caatgtattc agcgagttgc tcttcatgat aggacgggtg aggaatagaa cagattttgg
    24901 caaaaatatc ccacagcggc tgtggagata attgagacag ttcagacacg ttaagtctcc
    24961 ttgtcgatca cccgcaaaac agtattgcag gtcacagggt tagcagaaaa tgttgtcaac
    25021 acaagacagg cttgcgagat atgtattgag aataccactt tatcccgcgt cagggagagg
    25081 cagtgcgtaa aaagacgcgg actcatgtga aatactggtt tttagtgcgc cagatctcta
    25141 taatctcgcg caacctattt tcccctcgaa cactttttaa gccgtagata aacaggctgg
    25201 gacacttcac atgagcgaaa aatacatcgt cacctgggac atgttgcaga tccatgcacg
    25261 taaactcgca agccgactga tgccttctga acaatggaaa ggcattattg ccgtaagccg
    25321 tggcggtctg gtaccggggg cgttactggc gcgtgaactg ggtattcgtc atgtcgatac
    25381 cgtttgtatt tccagctacg atcacgacaa ccagcgcgag cttaaagtgc tgaaacgcgc
    25441 agaaggcgat ggcgaaggct tcatcgttat tgatgacctg gtggataccg gtggtactgc
    25501 tgttgcgatt cgtgaaatgt atccaaaagc gcactttgtc accatcttcg caaaaccggc
    25561 tggtcgtccg ctggttgatg actatgttgt tgatatcccg caagatacct ggatcgaaca
    25621 gccgtgggat atgggcgtcg tattcgtccc gccaatctcc ggtcgctaat cttttcaacg
    25681 cctggcattg ccgggcgttg ttctttttaa cttcaggcag gttacaatag tttccagtaa
    25741 gtattctgga ggctgcatcc atgacacagg caaacctgag cgaaaccctg ttcaaacccc
    25801 gctttaaaca tcctgaaacc tcgacgctag tccgccgctt taatcacggc gcacaaccgc
    25861 ctgtgcagtc ggctcttgat ggtaaaacca tccctcactg gtatcgcatg attaaccgtc
    25921 tgatgtggat ctggcgcggt attgacccac gtgaaatcct cgacgtccag gcacgtattg
    25981 tgatgagcga tgctgaacgt accgacgatg atttatacga taccgtgatt ggctaccgtg
    26041 gtggcaactg gatttatgag tgggccaccc aggcgatggt gtggcaacaa aaagcctgta
    26101 cggaagaaga tccgcaactc agtggtcgac actggctgca tgcggctacg ttgtacaaca
    26161 ttgccgccta tcctcatctg aaaggagatg acctggccga gcaagcgcag gctttatcaa
    26221 accgcgccta tgaagaggcc gcccagcgtc tgccgggcac gatgcggcag atggagttta
    26281 ccgtacccgg cggtgcgccc atcaccggct ttttgcatat gccgaaaggc gatggcccgt
    26341 tcccgacagt attaatgtgt ggtggtctgg atgcgatgca gacggactat tacagcctgt
    26401 atgaacgtta ttttgcgccg cgcggcattg cgatgctgac tattgatatg ccgtcggtgg
    26461 gcttttcttc aaaatggaag ctcacccagg actccagcct gttgcatcag cacgtcttaa
    26521 aggcgctgcc taacgtaccg tgggtggatc acactcgcgt cgcggccttt ggtttccgtt
    26581 tcggcgctaa cgttgccgtg cgtctggcat accttgaatc gccgcgtctg aaagcggttg
    26641 cctgtcttgg tccggtagtt cataccctgt tgagtgattt taagtgccag caacaggtgc
    26701 cggaaatgta tcttgacgtt ctggcgagtc gtttggggat gcatgatgct tccgacgaag
    26761 cgttacgcgt ggagctgaat cgctattcat taaaagtgca aggattgctg gggcgtcgct
    26821 gcccaacgcc aatgttatca ggctactgga agaacgatcc gttcagcccg gaagaggact
    26881 cacgcttaat cacctcatca tctgctgacg gtaaattatt agagatccca tttaacccgg
    26941 tgtatcggaa ttttgacaaa ggtcttcagg aaatcaccgg ctggatcgaa aaacgcttgt
    27001 gttaaaaatt tgctaaattt tgccaatttg gtaaaacagt tgcatcacaa caggagatag
    27061 caatgacgtt accgagtgga cacccgaaga gcagattgat caaaaaattt accgcactag
    27121 gcccgtatat tcgtgaaggt aagtgcgaag ataatcgatt ctttttcgat tgtctggctg
    27181 tatgcgtcaa cgtgaaaccg gcaccggaag tgcgtgaatt ctggggctgg tggatggagc
    27241 tggaagcgca ggaatcccgt tttacctaca gctaccagtt tggtctgttc gataaagcag
    27301 gcgactggaa gagtgttccg gtaaaagata ctgaagtggt tgaacgactg gagcacaccc
    27361 tgcgtgagtt tcacgagaag ctgcgtgaac tgctgacgac gctgaatctg aagctggaac
    27421 cggcgaatga ttttcgtgat gaaccggtga agttaacggc gtgagtgaaa tgtgccggat
    27481 gcatcacatc cggcaatatt cattaaaact gatacgtcat cccaaccgcg acagtatcat
    27541 cattattaat attcagctta ttatcgctat ccagttggtt gattttataa tcaacaaacg
    27601 ctgacatatt tttgttgaaa taatacgtag caccgacgtc gatataattg accagatctt
    27661 catcaccgat gccctcaata tctttccctt tcgataagac ataacccagc gatggacgca
    27721 gaccaaagtc aaactggtat tgagcgaccg cttcaaagtt ctgtgtctta ttggcaaagc
    27781 cgccagttat tggcgtcatt ttgcgtgttt cagaatagaa agttgccaga taaatattat
    27841 tggcatcgta tttcagacct gttgcccatg cttctgcacg cttgcctgtg ccacggcttt
    27901 gcaggttctg ctcgttggtg cgatctgagt tggtataggc cccactaatg gcgaaatcgc
    27961 tgccgccaaa gtcatatgtc aatgacgtgc cgaagccatc gccgttttgc tttttaacgt
    28021 cgcggttttc gtttttccct tgatattgca gggttaagtt caggccatcg ataacgccga
    28081 agaagtcggt gttccgatac gtcgccagac cgctggcgcg tttggtcata aagttgtcgg
    28141 tctgcgccga ggaatcgcca ccaaattccg ggaacatatc ggtccaggct tccacgtcat
    28201 acaacgcgcc caggttacga ccataatcga aagaacccaa atctttatat ttcaacccgg
    28261 caaaagcgag acgcgttttt tgctgtgcag tatcactctc cgctttattt ccggcaaact
    28321 ccgcttccca ccgaccataa ccagtcagtt gatcgttaat ttgagtttca cctttaaaac
    28381 caaaacggat ataactctgg tcgccatctt tactggcgtt atcactcata taatgcatgg
    28441 ctttaacttt gccatagaca tccagtttat taccgtcttt attatatatt tctgcagcct
    28501 gtacagatgc agatgccaca atgcccatca ccactaatgc cagagtgctc tttttcattt
    28561 tcattcctga ttttaattaa cgcgcgaata ttcagcggga gagccccgtt gaaaacagga
    28621 aagtttttaa cctgagattg ttaaagatat attacagatt aatgatattc tgaaaa
//
LOCUS       DAEBXN010000047        28509 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.047, whole genome shotgun sequence.
ACCESSION   DAEBXN010000047 DAEBXN010000000
VERSION     DAEBXN010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28509
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.047"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(<1..142)
                     /gene="yjdI"
                     /locus_tag="KJL99_002094"
     CDS             complement(<1..142)
                     /gene="yjdI"
                     /locus_tag="KJL99_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HBD4436818.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKS"
     gene            323..1954
                     /gene="dcuS"
                     /locus_tag="KJL99_002095"
     CDS             323..1954
                     /gene="dcuS"
                     /locus_tag="KJL99_002095"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HBD4436819.1"
                     /translation="MRHSLPYHILRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILNALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            1951..2670
                     /gene="dcuR"
                     /locus_tag="KJL99_002096"
     CDS             1951..2670
                     /gene="dcuR"
                     /locus_tag="KJL99_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HBD4436820.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDAPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            3241..4581
                     /gene="dcuB"
                     /locus_tag="KJL99_002097"
     CDS             3241..4581
                     /gene="dcuB"
                     /locus_tag="KJL99_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HBD4436821.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            4659..6305
                     /gene="fumB"
                     /locus_tag="KJL99_002098"
     CDS             4659..6305
                     /gene="fumB"
                     /locus_tag="KJL99_002098"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HBD4436822.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAENNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNLGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            6428..7057
                     /locus_tag="KJL99_002099"
     CDS             6428..7057
                     /locus_tag="KJL99_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HBD4436823.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLLEVTPVIIV
                     VPLLLATARRYPLTLLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVILLARFHCRQLRRYGLITG"
     gene            complement(7196..8617)
                     /gene="melB"
                     /locus_tag="KJL99_002100"
     CDS             complement(7196..8617)
                     /gene="melB"
                     /locus_tag="KJL99_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HBD4436824.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQSSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(8720..10075)
                     /gene="melA"
                     /locus_tag="KJL99_002101"
     CDS             complement(8720..10075)
                     /gene="melA"
                     /locus_tag="KJL99_002101"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HBD4436825.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICENMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPYIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKLGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPSWLH
                     R"
     gene            10358..11266
                     /gene="melR"
                     /locus_tag="KJL99_002102"
     CDS             10358..11266
                     /gene="melR"
                     /locus_tag="KJL99_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HBD4436826.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSSWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            11465..13732
                     /gene="adiA"
                     /locus_tag="KJL99_002103"
     CDS             11465..13732
                     /gene="adiA"
                     /locus_tag="KJL99_002103"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HBD4436827.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSIHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKHHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPRFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            14057..14818
                     /gene="adiY"
                     /locus_tag="KJL99_002104"
     CDS             14057..14818
                     /gene="adiY"
                     /locus_tag="KJL99_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HBD4436828.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            14955..16292
                     /gene="adiC"
                     /locus_tag="KJL99_002105"
     CDS             14955..16292
                     /gene="adiC"
                     /locus_tag="KJL99_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HBD4436829.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            16396..18039
                     /gene="eptA"
                     /locus_tag="KJL99_002106"
     CDS             16396..18039
                     /gene="eptA"
                     /locus_tag="KJL99_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HBD4436830.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILIGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAELIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            18036..18704
                     /gene="basR"
                     /locus_tag="KJL99_002107"
     CDS             18036..18704
                     /gene="basR"
                     /locus_tag="KJL99_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HBD4436831.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            18705..19805
                     /gene="pmrB"
                     /locus_tag="KJL99_002108"
     CDS             18705..19805
                     /gene="pmrB"
                     /locus_tag="KJL99_002108"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HBD4436832.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(19781..19870)
                     /gene="pmrR"
                     /locus_tag="KJL99_002109"
     CDS             complement(19781..19870)
                     /gene="pmrR"
                     /locus_tag="KJL99_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HBD4436833.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(19982..21484)
                     /gene="proP"
                     /locus_tag="KJL99_002110"
     CDS             complement(19982..21484)
                     /gene="proP"
                     /locus_tag="KJL99_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HBD4436834.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFMGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(21748..22626)
                     /locus_tag="KJL99_002111"
     CDS             complement(21748..22626)
                     /locus_tag="KJL99_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HBD4436835.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEGLRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            complement(22623..24851)
                     /gene="crfC"
                     /locus_tag="KJL99_002112"
     CDS             complement(22623..24851)
                     /gene="crfC"
                     /locus_tag="KJL99_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HBD4436836.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELCKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAEMESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            25254..25589
                     /locus_tag="KJL99_002113"
     CDS             25254..25589
                     /locus_tag="KJL99_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HBD4436837.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            25747..26190
                     /gene="yjdN"
                     /locus_tag="KJL99_002114"
     CDS             25747..26190
                     /gene="yjdN"
                     /locus_tag="KJL99_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HBD4436838.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            26323..27111
                     /gene="phnC"
                     /locus_tag="KJL99_002115"
     CDS             26323..27111
                     /gene="phnC"
                     /locus_tag="KJL99_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HBD4436839.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQLFDNERFDHLYRSINRIEENAKAA"
     gene            27136..28152
                     /gene="phnD"
                     /locus_tag="KJL99_002116"
     CDS             27136..28152
                     /gene="phnD"
                     /locus_tag="KJL99_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBD4436840.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLILGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            28258..28431
                     /locus_tag="KJL99_002117"
     CDS             28258..28431
                     /locus_tag="KJL99_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane channel protein of phosphonate
                     transporter"
                     /protein_id="HBD4436841.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLDVLVVSWQGAEMAPLTLIKDG
                     GNMATFAVMTPTY"
     gene            complement(28425..>28509)
                     /locus_tag="KJL99_002118"
     CDS             complement(28425..>28509)
                     /locus_tag="KJL99_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBD4436842.1"
                     /translation="RKSLSFSKSVELHDKVIGHYLNIKHYQ"
BASE COUNT         7322 a         7445 c         6957 g         6785 t
ORIGIN      
        1 acgactttcg tttgagatta aataacttgc cgttgccacg tacgcaattg ccagaatgct
       61 gacatatcgc agtgttgaaa tagacatcga tcttttcgcc ggtataacag cgataacccc
      121 cgtccagtag cgcctgatcc atgaccttcc ctctctcatc agtatgatta tcgctataag
      181 catagccccg gaatcttcat acagcactgg cagtttccgt tgccagagtc tcttccccta
      241 tatagccaat catttttact ccctttgaat tacccgcctc atcagagata atgcttaaga
      301 aatttgtcac acaaggaagc tgatgagaca ttcattgccc taccacatat tacgcaaacg
      361 tccgatgaaa ttgagtacca ctgtgatctt aatggtcagc gcggtactgt tctcggtgct
      421 attggtggtg catctgattt acttctcgca aatcagtgat atgacgcgag acgggctggc
      481 caacaaggca ctggcagtgg cgcgtaccct cgccgactcg ccggaaatcc gtcagggctt
      541 gcagaaaaaa ccgcaggaga gtggcatcca ggccatcgcg gaagccgtgc gcaaacgcaa
      601 cgatctgctg tttattgtcg ttaccgatat gcaaagtctt cgctactcac atcctgaagc
      661 ccagcgtatt ggtcagccat ttaaaggtga tgacatcctt aatgcgctga atggcgaaga
      721 aaatgtcgct atcaatcgcg gttttctggc gcaggcttta cgcgtattta cccccatcta
      781 cgatgaaaat cataaacaaa ttggcgtggt ggcgatcggc cttgagttaa gccgcgtgac
      841 ccaacagatc aatgacagtc gctggagcat tatctggtcg gtattatttg gcatgctggt
      901 cggactgatt ggcacctgca ttctggttaa ggtactgaaa aaaatccttt tcggcctgga
      961 accctacgaa atctccacgc tgtttgagca acgccaggcc atgttgcagt ctatcaaaga
     1021 aggcgtcgtt gccgtggacg atcgcggcga ggtcacgctg atcaacgatg ccgcacaaga
     1081 attgctgaat taccgtaagt cgcaggacga tgagaaactg tcgacgctca gccactcatg
     1141 gtcacaggtg gtagatgtct cggaagtgtt acgcgacggt acgccgcgcc gcgacgaaga
     1201 gattacgatt aaagaccggc tattactgat caacaccgtt ccggtgcgca gtaatggcgt
     1261 tatcatcggt gccatttcaa ccttcaggga caaaactgaa gttcgtaaac tgatgcagcg
     1321 actcgacggt ctggtcaact atgctgacgc acttcgtgaa cgatcccacg aatttatgaa
     1381 taaattgcat gtgattctcg gattattgca tctgaagagt tataagcagt tggaagatta
     1441 cattctcaaa acagccaata actatcagga agagattggc tctctgctgg gtaagatcaa
     1501 atctccggtt atcgctggtt ttttaatcag caagattaac cgcgcgaccg atttaggcca
     1561 tacgctgatt ttaaacagtg aaagccagct gccagacagc ggcagtgagg accaggtcgc
     1621 gacgctgatt accacattgg gaaatctgat agaaaacgcg ctggaagcat tagggccgga
     1681 acccggtggc gaaattagcg taacattgca ctaccgtcac ggctggctgc actgcgaagt
     1741 taacgatgat ggaccgggga tcgcacccga caaaatcgat cacatttttg acaaaggtgt
     1801 ctcgacaaaa ggaagcgagc gaggcgtcgg tttagcactt gtcaaacaac aggtagaaaa
     1861 tctcggcggc agcatcgccg tggaatcgga accggggatt ttcacacaat tttttgtcca
     1921 gataccctgg gacggggaga ggtcgaacag atgatcaatg tattaattat cgatgacgac
     1981 gcaatggtcg cggagctgaa tcgccgatac gtagcacaaa tcccaggctt tcaatgctgt
     2041 ggaacagcct cgacgctgga gaaagccaaa gagattatct tcaatagcga tgcgcctatc
     2101 gacctgatat tgctcgatat ctatatgcaa aaagagaacg ggctcgattt actgcctgtc
     2161 ctgcataacg cgcgttgcaa aagtgatgtg attgtcatct cctccgcagc cgatgcggca
     2221 accattaaag attcgctgca ttacggtgtc gtggattacc tgatcaaacc cttccaggct
     2281 tcccgctttg aagaggcgct caccggctgg cggcaaaaga aaatggcgct ggaaaaacat
     2341 cagtattatg accaggccga gcttgatcag ctaatccacg gcagcagctc caacgaacag
     2401 gatcctcgtc gcttgccaaa aggcttaacg ccgcagacgt tacgcacgct gtgccagtgg
     2461 attgacgcgc atcaggacta tgagttttca accgacgagc tggctaacga ggttaacatc
     2521 tcgcgtgttt cctgccgaaa atacctcatc tggctggtca actgccacat cttgttcacc
     2581 agtatccatt atggcgtcac ggggcgtccg gtttaccgtt accgtattca ggcagaacac
     2641 tactcactgc tgaaacaata ttgccaataa cggatcgctg gttatctgta agtaataatt
     2701 attaccgtaa tgcaaaatac ccctggcaca agttacatcg ttaatcagta aaacttgtgc
     2761 cagatcaaat ataattatcc ctccatcatc gttaaaaatt aatatctctt cagatgaacg
     2821 gtgtttttaa tttcaaaacg ataacaaaag ttaattaact attatgtcac ccgcattatg
     2881 tgtattttta cccacaaatg ggtagatcag attaatctat aaacctaatg atatctgccc
     2941 tgagaacaaa aaatagaccg ataaatatca ataagataac agcaaacaaa acattaacat
     3001 ctgcgcagta caaactataa acccatcgcc agagagtttt tctctctgaa aaagccgctt
     3061 atcacagtgc ataaatttgc cgctgcttta atcagccaat attcactgtg aggtatttgc
     3121 taaagccggt aacgaccaaa cggatattta gtcaggctct gaaaacagtt catacaaaac
     3181 agaacgtgac tgtgatctat tcagcaaaaa tttaaatagg attatcgcga gggttcacac
     3241 atgttattta ctatccaact tatcataata ctaatatgtc tgttttatgg tgccagaaag
     3301 ggtggtatcg cgctgggttt attaggcggt atcggtctgg tcattctggt cttcgtcttc
     3361 caccttcagc caggtaaacc accagttgat gtcatgctgg ttatcattgc ggtggtggcg
     3421 gcatcggcga ccttgcaagc ttcgggcggt cttgatgtca tgctgcaaat tgccgagaag
     3481 ctgctgcgcc gcaacccgaa atatgtctca attgtcgcgc cgtttgtgac ctgtacactg
     3541 accattcttt gcggtacggg tcatgtggtt tacaccattc tgccgatcat ctacgacgtc
     3601 gccattaaga acaacatccg tccggaacgt ccgatggcgg caagttctat cggtgcacag
     3661 atggggatta tcgccagtcc ggtgtcggtt gcggtcgtgt ctctggttgc gatgctgggt
     3721 aatgtcacct ttgatggtcg ccatcttgag ttcctcgatc tgctggcaat caccattcca
     3781 tcgacgttaa tcggtatcct ggcgatcggt atcttcagct ggttccgcgg taaagatctg
     3841 gataaagacg aagagttcca gaaattcatc tccgtaccgg aaaaccgtga gtatgtttac
     3901 ggtgataccg cgacgctgct ggataaaaaa ctgccgaaaa gcaactggct ggcaatgtgg
     3961 attttcctcg gggcaatcgc tgtagtcgcc cttcttggtg ctgattcgga cctgcgtcca
     4021 tccttcggcg gcaaaccgct gtcgatggta ctggttattc agatgtttat gctgctgacc
     4081 ggggcgctga ttattatcct gaccaaaacc aatcccgcgt ctatctcaaa aaacgaagtc
     4141 ttccgttccg gtatgatcgc catcgtggcg gtgtacggta tcgcatggat ggcagaaacc
     4201 atgttcggtg cgcatatgtc tgaaattcag ggcgtactgg gtgaaatggt gaaagagtat
     4261 ccgtgggcgt atgccattgt tctgctgctg gtttccaagt ttgtaaactc tcaggctgcg
     4321 gcgctggcgg cgattgttcc ggtcgcgctg gcgatcggcg ttgatccggc atacatcgtg
     4381 gcttcagcac cggcttgcta cggttattac atcctgccga cttatccgag cgatctggca
     4441 gcgattcagt ttgaccgttc cggcaccacc cacatcggtc gcttcgtcat caaccacagt
     4501 tttattctgc cggggttgat tggtgtgagc gtatcgtgcg tcttcggctg gatcttcgcc
     4561 gcgatgtacg ggttcttata aatgcacttt gcgtgccgcc cgtgactacg cggcacgcca
     4621 ttttcgaata acaaatacag agttacaggc tggaagctat gtcaaacaaa ccctttatct
     4681 accaggcacc tttcccgatg gggaaagaca ataccgaata ctatctactc acttccgatt
     4741 acgttagcgt tgccgacttc gacggcgaaa ccatcctgaa agtggaacca gaagccctga
     4801 ccctgctggc gcagcaagcc tttcacgacg cttctttcat gctacgtccg gcacaccaga
     4861 aacaggttgc ggctattctt cacgatccag aagccagcga aaacgacaag tacgtggcac
     4921 tgcaattctt aagaaactcc gaaatcgccg ccaaaggcgt gctgccgacc tgccaggata
     4981 ccggcaccgc gatcatcgtc ggtaaaaaag gccagcgcgt gtggaccggt ggcggtgatg
     5041 aagaagcgct gtcgaaaggc gtctataaca cctatatcga agataacctg cgctactcgc
     5101 aaaacgcggc gctggacatg tacaaagagg tcaacaccgg cactaacctg cctgcgcaaa
     5161 tcgacctgta cgcggtagat ggcgatgagt acaaattcct ttgcgttgcg aaaggcggcg
     5221 gctctgccaa caaaacgtat ctctaccagg aaaccaaagc cctgctgact cccggcaaac
     5281 tgaaaaactt cctcgtcgag aaaatgcgta ccctcggtac tgcagcctgc ccgccgtacc
     5341 atatcgcgtt tgtgattggc ggtacgtctg cggaaaacaa cctgaaaacc gtcaaattag
     5401 caagcgctca ctattacgat gaactgccga cggaagggaa cgaacatggt caggcgttcc
     5461 gcgatgtcca gctggaacag gaactgctgg aagaggccca gaaactcggt cttggcgcgc
     5521 agtttggcgg taaatacttc gcgcacgaca ttcgcgttat ccgtctgcca cgtcacggcg
     5581 catcctgccc ggtaggcatg ggcgtctcct gctccgctga ccgtaacatt aaagcgaaaa
     5641 tcaaccgcga aggtatctgg atcgaaaaac tggaacacaa tctaggtcag tacattccac
     5701 aagaactgcg tcaggccggt gaaggcgaag cggtgaaggt cgaccttaac cgcccgatga
     5761 aagagatcct cgcccagctt tcgcaatatc cggtttccac tcgtttgtcg ctcactggca
     5821 ccattattgt gggtcgagat attgcacacg ccaagctgaa agagctgatt gacgccggta
     5881 aagaacttcc gcagtacatc aaagatcacc cgatctacta cgcgggtccg gcgaaaaccc
     5941 ctgccggtta tccatcaggt tcacttggcc caaccaccgc aggtcgtatg gactcctatg
     6001 tggatctgct gcaatcccac ggcggcagca tgatcatgct ggcgaaaggc aaccgcagcc
     6061 agcaggttac cgacgcgtgt cataaacacg gcggcttcta cctcggtagc atcggcggtc
     6121 cggcggcggt actggcgcag cagagcatca agcatctgga atgcgtcgct tatccggagc
     6181 tgggtatgga agctatctgg aaaatcgaag tagaagattt cccggcgttt atcctggtcg
     6241 atgacaaagg taacgacttc ttccagcaaa tcgtcaacaa acagtgcgcg aactgcacta
     6301 agtaacctct tcggcccagc gcctggcagc atgctgccag gtgatccccc tgggccacct
     6361 cttttgcgat tgtaatttca tgcttgctgg tgaatagtca gtattttccc tgacttgcga
     6421 actcaccatg acccgcacac tcaagccgtt aattcttaac actggcgcac tggcgctaac
     6481 gttaatcctg atttataccg gcatttcggc ccatgacaaa ctcacctggc tgctggaagt
     6541 gacaccggtg attatcgtcg tgccgctact acttgccacc gccagacgtt acccgttaac
     6601 gctgctgctc tatacgctca tcttctttca cgccatcatc ctgatggtag gcgggcagta
     6661 cacctacgcg aaagtccccg ttggttttga ggtgcaggaa tggctggggt tgagtcgtaa
     6721 tccgtatgac aagctggggc actttttcca ggggctggtg cctgcactgg tggcacgaga
     6781 aattctcgtt cgcgggatgt acgtgcgcgg acgtaaaatg gtggcgtttc tggtctgttg
     6841 cgtggcgctg gcgataagcg cgatgtatga attaatcgag tggtgggcgg cactggcgat
     6901 gggccaggga gcggatgatt ttctcggtac ccagggcgac cagtgggaca cgcaatccga
     6961 tatgttctgc gcgctgcttg gcgcattaac gacagtgata ttgctcgctc gctttcattg
     7021 ccgccagcta cggcgctatg gcttgataac tggataacaa cgtgcacttt gttgatgccg
     7081 gatgcggcgt gaattcctta tccggtctac aaaatttgca aatccaaagc attacattaa
     7141 gacgtggcct gataaacata tcgcatcagg ccattttcgt tagctattgt ccacgtcagg
     7201 ctttcacatc actcactgca ccgaccggaa tatcagcatg aacaggctcg ggcggtactt
     7261 tgcgatattt atccagcaga tgaatctgga tcctgcgcag cgtgtcgcca ttgaggcgat
     7321 agaaacggaa gtagagaatc agcgttacca tgaaaaacag agtcggtaga gcaatcataa
     7381 taaactgcat acctaatagg gcttgcgtag actgttcaac attcggtaca tagccaatca
     7441 tccctaacac aaccgcaatg aaaaaagccg caaaggctga accgcccttc accaccatag
     7501 tctgcacgga gtaagcgatg ctttcacagc gtacgtgcag tttatattca ccgtaatcaa
     7561 cggtatctgc caccatgatg acctgtaata cccagaaaag cgccgttccc acattcagca
     7621 aaatacccgc aatcacaatg aggacgacgt tgtgatagct cattaatgcc attaacagga
     7681 gaacaccaca gcttaacacc ggaagaatag atgctccggc ccataaaatg cgtcgggata
     7741 atgatttaac taagcgtggg aagaatacta acgtcaccag gttagcagct cccgcatacg
     7801 acagataata ggggaacaaa tccgcatcac cgataacata tgagaaataa tagatagcaa
     7861 atccggtaat aatgttgctg gctacattat aagcaagagc catacccaag aggcatgaaa
     7921 gctgatcgtt tttataaata agcgcaacga tggctttaag tgtcagatgg cttccttcag
     7981 cagacgattg attgtctgac gaaaagactt catgcacatt gcgcagagtg atgatggttg
     8041 aaacaataaa aaaggcgatc aataccagag tgaacatctg aaagccaaat ccccgatcgc
     8101 caccgccgac ataattaaca aatggtagcg tcacacctgc cgtaacaaag cctgccagac
     8161 tggcaaaaaa acgcggataa ggaaccagtt gttcgcgttc acgtttatcg agcgtgatgg
     8221 ttggaaccag cgaccagaag ggaatatcca taatggtgta agtcatgccc cagaggatgt
     8281 aggtcacgca aacaaagaca atctgagtag taccttcaaa cagatgcgca ctaaagagga
     8341 gaaataagat tacagagttt gccaacgtac cgatcaggat ccagggtttg aacttacccc
     8401 atcgcgatcg cgtagcattt acaatccatc ccataatcgg atcgttaata gcatcccaga
     8461 tcctcgccac cagaaacaaa gtaccgacca aacccacaga cagcccgacg acatcggtgt
     8521 agtaatacat gaggtacata tacacaatgc cgatcgcaaa atccttcccg aacgctccaa
     8581 atccataact gagttttgta gtcattgaaa tgctcatagg gtatcgggtc gctgttacca
     8641 acgcccgcct tctgttattg gtatcgtcag aggtgtgcgc cggaataaat cccccggcgc
     8701 agtagcgttt agtcgcgttt taacggtgca accagcttgg cagccagtcg ccgtgggcgg
     8761 caatcaggtc gtcaacaaga gcatatattt cgtcaatgcc cagcacggcg gcagtatgcg
     8821 ggtccatcat cgcggcgtgg taaacacggt cgcgattttc cgtaagaata gcttcggtca
     8881 gcagcgtctg tacgttgatg ttggtttgca tcagggcggc cagatgcgaa ggtagcgtac
     8941 cgactttggt cggctgaatg ccattagcat caaccagaca ggctacttcc acgcaacatc
     9001 cttgtggcag gttatcaatc aaaccatcgt tacggacgtt gccgtaaatc acactcggct
     9061 cgccagtcca gatagcgttc atgattgtgc tggcatattc ccgtgacggt ttaatatcaa
     9121 tccgggaggc gtttttatac tcctccagct ctttatgcca gttcgccagc tgctcgacgc
     9181 agcgtttcgg gtactcatcc agcggtactt tataacgctc aatcaaatcc tcacgaccta
     9241 gcttaataaa ccacggcgta tattcggcaa aatgttctga tgattccgtg acgaagtagc
     9301 ccagcttttt gaacatttcg tagcgcacaa tattctggca gcgagtatta ccatgaatat
     9361 ttggcttcgg tgcctgccct gcttcataag ccgccagcag ttccgggtag agattcacgt
     9421 aactgccgtc ggcggttttg cgctccagct ccaggtaaaa cgccatatgg ttgatacctg
     9481 cgcaacggta acgcagcgta gccgggtcga tattgaggtc acgcgccagc tcttccgccg
     9541 ttccctgcac cgaatggcac agcccgacct gtttgatata cggatagcgg gcatacatcg
     9601 cccaggtatt catcgccatt gggttaacat agttgagcat ggtggcatcg gggcagactt
     9661 ccgtcatgtt ctcgcaaatt tgccacagat gcggaatggt acgtagcgcg cgcataatac
     9721 cgcccggccc caacgtatcg gcaatggttt gttccagacc atgccgctta cagacctcga
     9781 aatcagtcac cgtgcaaggt tcataaccgc caatctgaaa tgccaccacg acaaaatcgg
     9841 catcctctaa ggcttctttc tgttgggtgt ggcaggtgat tttgccgctg gcccctgctg
     9901 aatccatcag cttacgcacc acaatatgcg actcttccag gcgggtgggg tcaatgtcca
     9961 tcagggcaat atgcgccgtt ttcagcgcct cgcgatggaa cacatcacca agaatatttt
    10021 taacgaaaat cgtcgaacca gcgccgataa atgtaatttt gggtgcagac atcatgcaga
    10081 tctcctggct tgcttgaata acttcatcat ggcaggcgaa tctgcagaaa acctccggct
    10141 tccaggttga gcattcccat aaactcagat cctgcgtgaa gcagcagtaa atctgagttt
    10201 atgggaataa attacctgaa aagtagagtt ttcggcctca tgatgctcgg acagtcccct
    10261 cgcccctctg ggaagagggt gaaaaccgtg ctcccactcg cagtcatcct ccctcactcc
    10321 tgccataatt ctgatattct aggaaagaga gccatccatg aatacagata cgtttatgtg
    10381 cagcagcgac gaaaaacaga cccgcagccc gctgtcgctg tattcggaat accagcggat
    10441 ggagattgag tttcgcgcac cgcatatcat gcccaccagc cactggcatg gtcaggtcga
    10501 agtgaatgtg cctttcgatg gcgatgtgga atacctgatc aacaatgaaa aagtgaatat
    10561 caatcagggt catatcacgc tgttctgggc ctgtacaccg caccaactaa cagataccgg
    10621 aacctgtcag agcatggcga tttttaatct gccgatgcat ctgtttctct cctggccgct
    10681 ggataaagac ctgattaacc acgtcactca cggcatggtg atcaaatcac ttgccacaca
    10741 gcaacttagc ccgtttgaag tacgccgctg gcagcaggaa ttaaacagtc cgaatgagca
    10801 aattcgccag ctcgccattg atgaaattgg cctgatgctc aagcgattta gcctctctag
    10861 ctgggaaccg attctggtca ataaaacctc gcgcacacac aaaaacagcg tctcgcgcca
    10921 tgcgcaattt tatgttagcc agatgctggg ctttattgcc gaaaactatg atcaggcgct
    10981 gaccatcaac gatgtggctg agcacgtcaa acttaacgcc aactatgcaa tggggatatt
    11041 ccagcgggtc atgcaattga cgatgaaaca gtacattacc gcgatgcgca tcaaccacgt
    11101 tcgcgcgtta ctgagcgata ccgataaaag tattctcgat attgccctga cggcaggctt
    11161 tcgttcaagt agtcgttttt acagcacgtt cggcaaatat gtcggcatgt cgccgcaaca
    11221 ataccgcaaa cttagccaac agcgccgcca gacgtttccc ggctaaagac gatatcagta
    11281 tcagccaaaa aaatagttta gccgtcgata ataagcagtg aaaataattt aaaataatca
    11341 cataacgtat tgtttataaa actaaatatt tcaaaaatgt ttgtttttca cgcgctttac
    11401 agcccgaaaa ggccggaaga tacttgcccg caacgaagat tccttcataa ccgggtaagc
    11461 aatgatgaaa gtattaattg ttgaaagcga gtttctccat caagacacct gggtcggtaa
    11521 cgccgttgag cgtctggcag atgctttaag ccagcaaaat gttaccgtga ttaaatccac
    11581 ctcctttgat gatggttttg ccattctctc ttcaaacgaa gccattgact gcctgatgtt
    11641 cagctatcaa atggagcatc cggacgaaca tcaaaacgtc agacaattga tcggtaagct
    11701 tcatgagcgc caacaaaacg tgccggtctt cctgttgggc gatcgggaaa aagccctcgc
    11761 cgcaatggac cgggatctgc tggagcttgt cgatgaattc gcctggattc tggaagatac
    11821 cgccgacttt atcgccggac gcgccgttgc cgcgatgacc cgctaccgcc agcagctgtt
    11881 gccgccactg ttcagcgcgc tgatgaaata tagtgacatc catgaatatt cctgggcagc
    11941 gccaggccac cagggcggcg ttggttttac caaaacaccc gccggacgtt tctaccatga
    12001 ctactatggt gaaaatctgt tccgtaccga catgggcatc gaacgaactt ccctcggttc
    12061 tttgcttgac catactggcg catttggcga aagcgaaaaa tatgccgcac gcgtatttgg
    12121 tgccgatcgc tcctggtcgg tagtcgtcgg tacttccggc tctaaccgca ccatcatgca
    12181 ggcttgcatg accgataacg atgtcgtggt cgttgaccgt aactgccata aatccatcga
    12241 acaaggtttg atgctgacag gcgcgaaacc ggtctatatg gtgccaagcc gcaaccgcta
    12301 cggcattatc gggccaatct atccgcagga aatgcaacct gaaaccttgc aaaagaaaat
    12361 cagtgaaagc ccgctgacca aagacaaagc cgggcaaaaa ccgtcttact gcgtggtaac
    12421 caactgcacc tatgacggcg tgtgttataa cgctaaagaa gcgcaggatc tgctggaaaa
    12481 aacctccgat cgtctgcact ttgacgaagc ctggtacggc tatgcacgtt tcaacccgat
    12541 ctatgccgat cactatgcca tgcgcggcga acctggcgat cacaacggtc ctaccgtttt
    12601 cgccacccac tccatccaca aactgctgaa tgcgctgtca caggcttctt atattcatgt
    12661 acgtgaaggt cgtggggcga ttaacttctc ccgcttcaac caggcctaca tgatgcatgc
    12721 caccacctcc ccgctgtatg ccatctgcgc atccaacgac gtggcggtgt cgatgatgga
    12781 cggcaacagc ggcctgtcac tgacacagga agtgattgac gaagcggttg atttccgtca
    12841 ggcgatggcg cggctatata aagagttcac cgctgacggt agctggttct tcaaaccgtg
    12901 gaacaaagaa gtcgtcaccg acccacaaac cggcaaaacc tatgactttg ctgacgcacc
    12961 aaccaaactg ctgaccaccg ttcaggactg ctgggtaatg catccgggcg aaagctggca
    13021 cggcttcaaa gatattccgg ataactggag tatgctcgac ccaattaaag tcagcatcct
    13081 tgctccggga atgggtgaag atggtgaact ggaagaaacc ggtgttccgg cggcgctggt
    13141 cactgcctgg cttggtcgcc acggcattgt gcctacccgc accactgact tccaaattat
    13201 gttcctgttc tctatgggcg taacccgtgg gaaatggggg actctggtta acaccctttg
    13261 ctccttcaaa caccactatg acgccaacac accgctggcg caggtgatgc cggaacttgt
    13321 tgaacaatat cctgacactt acgcgaacat ggggattcac gatctgggtg acaccatgtt
    13381 tgcctggctg aaagaaaaca accctggcgc acggttgaac gaagcctatt ccggcctgcc
    13441 ggtggcggaa atcaccccgc gtgaagcgta caacgcgatt gtcgacaaca atgtcgaact
    13501 ggtatccatt gaaaatctgc caggacgcat cgcggcaaac tcagttatcc cgtatccgcc
    13561 aggaatcccg atgctgctgt ctggtgaaaa cttcggcgat aaaaacagtc cgcaagtaag
    13621 ttatttacgc tcgctgcaat cctgggacca ccatttccct agatttgaac acgaaactga
    13681 agggactgaa attattgacg gtatttacca cgttatgtgc gtgaaagcgt aaccactatt
    13741 ccgctgaagg cgtaattgtt taaataacat tacgccgcct ggccttaggc cttttgagta
    13801 tggcaacgtt ttcataaaaa ttgctgcaaa caaaaatgtc atactttttg cgcggcccca
    13861 ccccacgctt ttttttgcct gttatttatc ctgtaaaaaa atatgtacat gagaaattac
    13921 tataaaaatt tgtaatatta gtaaaactcg ttatttttat gcatgtttat attcatcatg
    13981 caattatata accatttccc ggtatcgctt tgctttagcg agaactggtc ttttattcgc
    14041 actcaggagt acatgtatga ggatttgcag cgaccaacct tgtattgttt tattgactga
    14101 aaaagatgtc tggataaggg tgaatgggaa agaacctatt agccttaaag ctaaccatat
    14161 ggcgttatta aattgtgaaa ataatattat cgacgtctcc tctcttaaca acactttggt
    14221 tgctcatatt agtcacgaca tcatcaaaga ttacctccgg tttctgaata aagatctctc
    14281 gcaaatacca gtatggcaac gtagcgctac gcccatactc accctgccat gcctgacgcc
    14341 agacgtcttt cgcgttgccg cgcaacacag catgatgccc gcagaaactg agtcagaaaa
    14401 ggaacgaaca cgcgcattat tattcacggt gctatcccgt tttctcgaca gtaaaaaatt
    14461 cctttcatta atgatgtata tgttacgtaa ttgtgtaagt gacagcgttt atcaaattat
    14521 tgaaagcgat attcacaaag actggaatct tagtatggta gccagttgtt tatgtcttag
    14581 cccaagtctg ttaaagaaaa agttgaaaag cgaaaacacc agttatagcc aaataatcac
    14641 cacctgccgc atgcgttatg ccgtaaatga attaatgatg gacggtaaaa atatctctca
    14701 ggtatcacag tcctgcggct acaacagtac gtcgtacttt atttctgtct ttaaagactt
    14761 ctacggtatg acgccgctgc attatgttag tcagcacaga gaacgcactg tcgcctgatt
    14821 tttaacctta acgaagagct atattaataa cggcatcagc gataacccgg tcgataataa
    14881 ttcaactatc gaatgcaggc gtatgatatg acgtaattta ttgtcacgaa gctcgccttc
    14941 gcaggagttt aattatgtct tcggatgctg atgctcacaa agtgggctta atccccgtca
    15001 ccctgatggt gtcggggaat attatggggt caggtgtttt tctgttacct gcaaacctgg
    15061 cctccactgg cgggattgcc atttatggat ggttggtgac gattatcggt gcgctggggc
    15121 tctcgatggt atacgccaaa atgtcgttcc tcgacccaag tcctggtggt tcttacgctt
    15181 acgcccgccg ctgctttggc ccgtttctcg gttatcaaac caacgtcctc tactggctgg
    15241 cctgctggat cggcaatatc gccatggtgg tcattggcgt aggatattta agttacttct
    15301 tcccgattct gaaagatcca ttggtattaa ccatcacctg cgtcgtggtg ctgtggatct
    15361 tcgtcctgct gaacattgtc ggtccgaaaa tgatcacccg cgtgcaggcc gttgccactg
    15421 tgctggcgct gatccccatc gtcgggattg ccgtatttgg ctggttctgg ttccgtggtg
    15481 aaacctatat ggcggcatgg aacgtcagcg gcctgggcac cttcggtgcg attcaaagta
    15541 cccttaacgt tacgctgtgg tcgttcatcg gtgtggaaag tgcctccgtt gccgcaggtg
    15601 tggtgaaaaa cccgaaacgc aatgtcccta tcgccaccat tggtggggta ttgattgccg
    15661 ccgtttgcta tgtgctctcc accaccgcga ttatggggat gatccctaat gccgcactgc
    15721 gcgtttctgc ctcgccattc ggtgatgccg cacggatggc gttgggtgac accgctgggg
    15781 ccattgtttc cttctgcgca gctgcgggtt gcttaggttc actgggcggc tggacgttgc
    15841 tggcgggtca aacggcgaaa gccgctgccg atgacggact gttcccaccg atttttgccc
    15901 gtgtaaataa agcgggtacg ccagtggcgg ggctgattat cgtcggtatt ttgatgacca
    15961 tcttccagct cagcagcatt tcaccaaacg cgaccaaaga gttcggtctg gtttcttccg
    16021 tctcggtcat ctttacactg gtgccatatc tttacacctg tgcggcgtta ctgctgctcg
    16081 gccacggtca ctttggtaaa gcacgcccgg catatctggc tgttactacc attgccttcc
    16141 tctactgcat ttgggccgtg gtggggtccg gggcgaaaga ggttatgtgg tcatttgtca
    16201 ccctgatggt catcaccgcc atgtatgcgc tgaattacaa ccggctacat aaaaacccgt
    16261 atcccttaga tgcaccaata agcaaagatt aattcccctt aatccagcaa acataaaagc
    16321 caaccttaag aacttaaggt tggcttaatt ttgctttgcg agcatatgcg cactttgttc
    16381 gatggaaaca ccgtgatgtt gaagcgccta ctaaaaagac cctctttgaa tttactcgcc
    16441 tggctattgt tggccgcttt ttatatctct atctgcctga atattgcctt ttttaaacag
    16501 gtgttgcagg cgctgccgct ggattcgctg cataacgtac tggttttctt gtcgatgccg
    16561 gttgtcgctt tcagcgtgat taatattgtc ctgacactaa gctctttctt atggcttaat
    16621 cgaccactgg cctgcctgtt tattctgatt ggcgcggctg cacaatattt cataatgact
    16681 tacggcatcg tcatcgaccg ctcgatgatt gccaatatta ttgataccac tccagcagaa
    16741 agttatgcgc tgatgacacc gcaaatgtta ttaacgctgg gattcagcgg cgtgcttgct
    16801 gagctgattg cctgctggct aaaaatcaaa cctgccacct cgcgtctgcg cagtgttctt
    16861 ttccgtggag ccaatattct ggtttctgta ctgctgattt tgctggtcgc cgcactgttt
    16921 tataaagact acgcctcgtt gttccgcaat aacaaagagc tggtgaaatc cttaagcccc
    16981 tctaacagca ttgttgccag ctggtcatgg tactcccatc agcgactggc aaatctgccg
    17041 ctggtgcgaa ttggtgaaga cgcgcaccgc aacccgttaa tgcagaacga aaaacgtaaa
    17101 aatttgacca tcctgattgt cggcgaaacc tcgcgggcgg agaacttctc cctcaacggc
    17161 tacccgcgtg aaactaaccc gcggctggcg aaagataacg tggtctattt ccctaatacc
    17221 gcatcttgcg gcacggcaac ggcagtttca gtaccgtgca tgttctcgga tatgccgcgt
    17281 gagcactaca aagaagagct ggcacagcac caggaaggcg tgctggatat cattcagcga
    17341 gcgggcatca acgtgctgtg gaatgacaac gatggcggct gtaaaggtgc ctgcgaccgc
    17401 gtgcctcacc agaacgtcac cgcgctgaat ctgcctgatc agtgcatcaa cggcgaatgc
    17461 tatgacgaag tgctgttcca cgggctggaa gagtacatca ataacctgca gggtgatggc
    17521 gtgattgtct tacacaccat cggcagccac ggtccgacct attacaaccg ctatccgccg
    17581 cagttcagga aatttacccc aacctgcgac accaatgaga tccagacctg taccaaagag
    17641 caactggtga acacttacga caacacgctg gtttacgtcg actatattgt tgataaagcg
    17701 attaatctgc ttaaagaaca tcaggataaa tttaccacca gcctggttta tctttctgac
    17761 cacggtgaat cgttaggtga aaatggcatc tatctgcacg gtctgcctta tgccatcgcc
    17821 ccggatagcc aaaaacaggt gccaatgctg ctgtggctgt cggaggatta tcaaaagcgg
    17881 tatcaggttg accagaactg cctgcaaaaa caggcgcaaa cgcaacacta ttcacaagac
    17941 aatttattct ccacgctatt gggattaact ggcgttgaga cgaagtatta ccaggccgcg
    18001 gatgatattc tgcaaacttg caggagagtg agtaaatgaa aattctgatt gttgaagacg
    18061 atacgctgtt attgcaggga ctgattctgg cggcgcaaac cgaaggctac gcgtgcgatg
    18121 gcgtgacaac cgcgcggatg gcggaacaaa gccttgagga cggtcattac agcctggtgg
    18181 tactggattt agggttaccc gacgaagatg gactgcattt tctcgcccgt atccggcaga
    18241 aaaaatacac cctgccggta ctgatcctca ccgctcgcga tacgctgacc gacaaaatcg
    18301 ccgggctgga tgtcggtgcc gatgactatc tggtgaagcc ttttgcgctg gaagagttac
    18361 acgcccgtat ccgcgccctg ctacgacgcc ataataatca gggcgaaagt gagctgattg
    18421 ttggcaatct gacgctgaat atgggtcgcc gtcaggtatg gatgggcggt gaagagttga
    18481 ttctgacgcc caaagaatat gctctgctgt cacggttaat gctcaaagca ggcagtccgg
    18541 tacatcggga aattctctac aacgacatct ataactggga caatgaaccc tcgaccaaca
    18601 ccctggaagt gcatatccac aatctgcgcg acaaagtggg caaagcccgt atccgcaccg
    18661 tgcgcggctt tggctatatg ctggtcgcga atgaggaaaa ctaattgaat ctgatgcgtt
    18721 ttctgcgccg acctatatcg ctgcgccaac ggctgatatt gaccatcggg gccattttgt
    18781 tggtgtttga gctgatcagc gtcttctggc tatggcatga aagtaccgag cagattcagc
    18841 tgtttgagca ggcgctacgc gacaatcgca acaacgatcg ccacatcatg cgtgagatcc
    18901 gcgaggcggt cgccagcctg attgtccccg gcgtctttat ggtcagcctg acgctattta
    18961 tctgctatca ggcggtacgc cgcatcaccc gcccgctggc ggagctgcaa aaagagctgg
    19021 aagcgcgcac cgccgacaac ttaacgccca ttgccattca cagcgccacc ctcgaaatcg
    19081 aagcggtggt ttcggcgttg aacgatctgg tcagtcgcct gaccagcacg ctggataacg
    19141 aaaggttgtt taccgctgac gtcgcgcacg aactgcgaac gccactggcg ggggtgcgtt
    19201 tgcatctgga actgctggcg aaaacgcatc acattgatgt agcaccgtta gtggcacggc
    19261 ttgatcagat gatggagagc gtctcccagc tgctgcaact ggcgcgtgcc ggacagtcat
    19321 tttcttccgg taattatcaa catgtaaaac tgctggaaga tgtcattctc ccctcgtatg
    19381 acgaactcag taccatgctc gaccagcgac agcaaaccct gctattgcca gagagcgccg
    19441 ccgacatcac cgttcagggt gatgcaaccc tgctgcggat gttattgcga aacctggtag
    19501 aaaacgccca tcgttacagc ccgcaaggca gcaacattat gattaagctg caagaagatg
    19561 gcggagcggt catggcagtt gaagatgaag gaccaggtat tgatgagagt aaatgcgggg
    19621 agttgagtaa agcgtttgta cgtatggaca gccgttatgg cgggattggt ctggggttaa
    19681 gtattgtcag ccgcattaca cagttgcatc acgggcagtt tttcctgcaa aaccggcaag
    19741 agacttccgg cacgcgggcc tgggtacggc tgaagaaaga tcagaacgtg gcaaaccaga
    19801 tataaagaaa gctgctgacc accagcacgc tgaacacggt agttaaactt tcataaacac
    19861 ggtttttcat tgcactctcc tccgttaacc tggaggagag tatgcgcgtc agagattaag
    19921 tgaaccttaa gagttcaatt ggcctgataa gacagcgtca catcaggcca tccgtttcag
    19981 cttattcatc aattcgcgga tgttgctgca ccaggcgggt acgtttcgcc tgcaaatcgg
    20041 caatctcgtg gtcaatatta tcgattttct gctcgatatt atcgtaatgc tcgacgagaa
    20101 tttccttcgc ttcctgtata tctgacgccg ccggtgtcgc acctttcaac ggacgatttg
    20161 ccgtctcttt catggttacg ccggtgatta aaccaatcac cgccactacc atcaggtaat
    20221 aggcaggcat catcagattc tgcgagcttt cgaccagcca ggccgccagt gttggcgtca
    20281 gaccggcaac cagcaccgaa atattaaatg ccgccgccag cgcgctgtaa cggatatgcg
    20341 tcgggaacat cgctggcaag gtagaagcca taacgcccat aaagcagtta aggatcaccg
    20401 ccagcatcag taacccggca aaaatcaggc cgatgacgtt actgttaatc agaataaacg
    20461 ccgggatcgc caacacaaac agtgcaacac taccaagtag cacaaacgga cgacggccaa
    20521 aacggtcact cagcaagccc atcaccggct ggacaaacag cataccgatc ataatggcga
    20581 taataatcag caccccgtgg tcttcggagt aatgcaggtt atgcgacaaa taactcggca
    20641 tataggtcag caacatgtag taagtcacgt tggtggcaat taccagacca atacatgtca
    20701 acaggctgcg ccagtattta gtggcaatct ctttaaacga gactttcggg ccatcctgca
    20761 aaccttcgcg gtcgccctgt tccagtttat caacatgctg ctggaacgcc ggagtttctt
    20821 ccagcgcatg gcgcaggtaa agcccgataa tccctaacgg cagagcaata aagaacggaa
    20881 tacgccagcc ccagtcgagg aagttcgctt cgccgacaat ggtcgaaatt aacaccacca
    20941 cgcccgcacc cagcacaaac ccggcaatag aaccgaagtc cagccagctg cccataaagc
    21001 cacgtttacg gtcaggggag tattccgcaa caaatatcga cgccccggta tattcaccac
    21061 cgaccgagaa accttgtgcc atcttacaga tcaacagcag aatcggtgcc caaataccaa
    21121 tcgtgtcgta ggacggtatt aagccaatac agaacgtact gatcgacata atcacaatag
    21181 tgatagcgag gatcttctgg cgaccatatt tatcgcccaa cataccaaag aagagtccgc
    21241 caagcggtcg aatcagaaag ggaacggaga aagtggcaag tgcagcaacc atctgcacgc
    21301 tggggtcagc ccccgggaaa aaaactttac ctaatgcgta agcaacaaaa ccataaacac
    21361 caaaatcaaa ccattccatt gcattaccca gtgatgctgc ggtaatggct ttacgcagtt
    21421 taccgtcatc aataatggtg acatcacgaa gggtaatcgg ttttactttt ttccttttca
    21481 gcatagcttt cctcgcagag ttggcccatt tccgcaaacg ccagaaaaat cagcgcgacg
    21541 gccgcaaaag gggagcggga attgcaggat gcaatctctg taacaagcgt agcgggtttg
    21601 cttacaccct ccggttaagg aatgtacaat caaaccagtt gacgcctgaa ttgggcagtt
    21661 aatgaccttt ttaccctacc agtgtttaaa tttgtgatca acttcacaca tggatttcgt
    21721 ttaggactca ttgatgtaac tgattattta cgccaaattt acagagaaga tttcctcgac
    21781 cattgccccc accaaacgct gcgctgaaca gagtcgtggc atccccagcg cgacagtttg
    21841 ccagcgttgg gtcactgacc agcaaacggg ttgtttatct gccgtggtcc actttcccag
    21901 ccaacttaaa aaatcagcat gatccccgcc cgtcggaacg gtcgaagagg caaaccactg
    21961 atggtaatag tgacgcgtag tcggtgccgc attgatactc tctgccagat gttgtgtcgc
    22021 tttctcgcgc aaatcaccca gcaacaacac caccgcttgc gggcttccca gacggcaggc
    22081 atcgccaaat gcaccccagc gcaacccctc cagaacgata aaacagcgcc caggcaacga
    22141 ccaggcgtta tacgccccag cacgccagcg ggtaaaaatt tgctcgctct gatcacctgc
    22201 ctgcaccagt aggcctcgtt gcgttaacgc cttttccttc cagcgacaac gggctgaaaa
    22261 ccactcatac aaggcggcaa tttgctgggg gagctccggg aaagcagagt gctggcggtg
    22321 atccattttt tgcagcagcg ccagatgctg cgcgatctgc gtcagcgcca gatgaccggg
    22381 atccagcatc gacgccagtt tttccaccat tgtcagtcgc aggctaagcg gcgcatttaa
    22441 catgccgccg ttcgtccatg tctgaagctg cacacgcgaa agaagttcct gcatcagtcg
    22501 ttcacgaaac tgctgctgat gggcttgagg atggcgggca tcatcgccct gcgccagatc
    22561 cactaaaaag cgcggatgaa ccgactccag cacgcgaccg gggccgtcca gtaacgtctt
    22621 ggtcaatatc gttctgccgt gaaaagtgtt tgaatatcat cgcgtaacag ctgagcgtcg
    22681 gtgtaaatcc agctgtgagt catcacagtc tgctgcaatt gctgctgcat cagcctgacc
    22741 accgattcat tttgttgacg cagagccaga ctttcgcgta aacgcgtctg taattctgtc
    22801 aaacatgaag cgaactcagc gaaaaaagta ttcatgcctg ccgtaacaga ttcatcgacc
    22861 tgctctgcca gaactttagc catttgttgg caatagagat cgacttctgc gcttaatgct
    22921 cgttgcaaca cactgtaatc aaccgtttct gtcggggatt tctcatttcc ccgtccccag
    22981 tcgggctgat tcaaccagcg cgaaaaagtc tcacgcacaa cgcctaaacg cgtgctctgc
    23041 tcgtccgttg cttcctggcg cgaaatgact tcactgaaca gctggcgagt gttgaagtgg
    23101 ggaactacgc cgtgaaaaac aggaaaatga aacccaggac gaaaccctga ctcgctcaat
    23161 tccattttga cttgttgctc aatggggcga ataacatcgg ttaacacccg gcaaagggtg
    23221 gattccatct cggcaaaacg cagcgtaaag tcgcgactga tggtgttctg cgccgtctgt
    23281 aacagtgtct cacagcgggt acgaatctcg cttaacggct ccgaatcatc ctgaaacaag
    23341 gcggctaact gcgcattcac cgcatcttgc tgttgacgca gaaagtggtt ggcggaggtc
    23401 agggccagct cgatttcatg tttaatctcg ccgctcacct gcgcctgatt gagttgcaat
    23461 agctgcaaac tttcttcgac ctgatggata ttttgccgca attgttcaca agcgacgttt
    23521 aacccgtgcg cacgaaaatc caggtattcc cgcgcctgct gcgcgtaatt caacagttta
    23581 tgcgcagcag atcgcagagc atacaatgag gcgttagcgt aagcggcatg aagcaacgcc
    23641 tgaattggct gggcgaacag cgaatcttcc cacaactgat cggcagcatg acgaatatgt
    23701 tcgaggtccg ccagatcggc atgacgccag cgcctgccga gcgcggcatg ggcaaaatct
    23761 tccacccagc gttgttgctc tggcgctggt aacttaccgt tgttggctaa ctcatagcgc
    23821 gcccgattcg ccaggtagcc ccacatcgac gacaccggga atatctgctg tggcgtaata
    23881 cagcctttca tcagcgtccc ggaaatcagt gcccgcacct ggtcggcgtc gtcactgtta
    23941 cgatcctgtt gatcgaactt attgaccagc acatacagcg gcaccgattg ccccaccgcc
    24001 aaaatcgcct cacggacctc ttcatcggag atcgatttca gttgcgtata atccagcacc
    24061 gccagtaccg ccgaggcgcg tgccagctgc tggttaagca ttttttgcag atgcggttgc
    24121 ccggcttcat ttggcccggg ggtatccagt aacgtcaact gaccgggata actttccagc
    24181 cccgccagat ggacaaactc cacttcaatc acgggaatat gctcaatggc ggcgtaagca
    24241 gaaaaaggaa aatcgacgtc cagcgccttc gccagtcgca ctaaatcatt caaacttttc
    24301 agacaatgaa aaataggctg ggcacccaga taatattttt cgaaagcgac gccattttcg
    24361 atccgctgca taagcgcacg catatcttta tctatttcca gcacatcggt cagatgctta
    24421 atatcgcaat cacgcaggcg ctgttgtaat tgttgaatta aacaatcgat tggcgcgaca
    24481 tgtgaaaaat gcagtaccgg ttccttttgc ccgggcgtat ggcgaataag cgtcggcagc
    24541 gcagtcattg ggcgattacg attaggcaga acctccgtac caacaatggc attaatggtg
    24601 gttgatttcc ctgctttcat ggtaccgaca attgccagca ccatttccag tcgggaaatt
    24661 ttacacaact cattattcag catcgcgtga cgttcggcga tattaggctg actccagggt
    24721 aaagccagtt gtggcgcgtc gtctccgggt acagagagag gcattttttc cagtaactgc
    24781 aactgttggc gagaaagctg taacaggcgt tcagcctcct gacttaactc atacagggtc
    24841 tgtgtgtaca tagaaaattc ttccttaaag caaattttgt tacttttatt tagccagatt
    24901 gtttttgagt tctgttttcg gcttttataa ttactgcaag aaataatttt atatttagtg
    24961 tgttgttttt ttatcagaat aaataacgtc ttctgatgcg tttaaaacgt cagaaagata
    25021 aaaatatcat gtgaataaaa aaaagaacaa gtagagcatt aacattatct taaataataa
    25081 atagaggcaa aaagattatt ttctttttgc gtttcctttc aaatgaaaac gatcgtcgtc
    25141 taaaatcagc agtacccccg acaaactcag ggattttgtg tataattgcg gcctttttcg
    25201 gcaatctgcc gttttttggc gcttttgccc tgctgacttt tgaggaaatc cacatgtcat
    25261 taccacactg cccaaaatgc aactccgaat acacttacga agataacggc atgtacatct
    25321 gcccggaatg tgcctacgaa tggaacgacg cagaacctgc acaggaaagc gacgagctga
    25381 tcgttaaaga tgctaacggc aatctgctgg ctgacggcga cagcgttacc atcattaaag
    25441 atctgaaggt gaaaggtagc tcttcgatgc tgaaaattgg caccaaagtg aaaaacatcc
    25501 gcctggttga aggcgaccat aacatcgatt gcaaaatcga cggttttggt ccgatgaaac
    25561 tgaaatctga gtttgtgaaa aagaactgat tgtattgtga tcggtaagcc ggataaggcg
    25621 ctcgcgccgc atccggcaac gatgccgact gcctgatgcg acgcttgcgc gtcttatcag
    25681 gcagcctctc atctgtataa atttcgaact acacttaact ggcttctctt aactgaggtc
    25741 accatcatgc cgttaagtcc ctacctctct tttgccggta actgttccga cgcgattgcc
    25801 tattatcaac gtacgttggg cgcggaactg ctctataaaa tcagcttcgg cgaaatgccg
    25861 aaatcagcgc aggacagcgc cgagaactgc ccttccggaa tgcaatttcc cgataccgcc
    25921 atcgctcatg ccaacgtgcg cattgccgga agcgacatca tgatgagcga tgctataccg
    25981 tcaggaaaag ccagctactc cggctttacg ctggtgctcg attcgcaaca ggtcgaagaa
    26041 ggaaaacgct ggtttgataa ccttgccgct aacggaaaaa tcgaaatggc ctggcaggaa
    26101 actttctggg cgcatggctt tggcaaagtc accgataaat ttggcgtacc gtggatgatt
    26161 aatgtcgtca aacaacaacc aacgcaataa cccgccggga ggcccgccct cccgcactgt
    26221 catcgaattc ccgttaactc ttcatctgtt agtcactttt aattaaccaa atcgtcacaa
    26281 taatccgcca cgatggagcc acttttttag ggaggctgca tcatgcaaac gattatccgt
    26341 gtcgagaagc tcgccaaaac cttcaatcag catcaagcgc tgcatgcggt tgatctgaac
    26401 attcatcaca gtgaaatggt ggctctgctt gggccgtcgg gttccggcaa atccaccctt
    26461 ttacgtcatt taagcggttt gattaccggc gataaatccg ccggtagcca tatcgagcta
    26521 ctgggccgca ctgtccagcg cgaaggccgc ctggcccgcg atatccgcaa aagccgcgcc
    26581 aacaccggct acatctttca acaattcaac ctggtgaacc gcctgagcgt actggagaac
    26641 gtgctgattg gcgcgctcgg cagcacgccg ttctggcgca cctgttttag ctggttcacc
    26701 ggcgagcaga aacagcgcgc gttacaggcg ctgacccgcg ttggcatggt gcattttgcc
    26761 catcagcgcg tttccaccct ctccggcggc cagcagcaac gtgtggcgat tgcccgtgcg
    26821 ctgatgcagc aggcgaaagt gattctggcc gatgaaccca tcgcctcgct ggacccggaa
    26881 tcagcgcgca tcgtgatgga caccctgcgc gacatcaacc agaacgacgg catcaccgtg
    26941 gtcgtcacgc tgcatcaggt ggattacgcc ctgcgctact gcgaacgcat cgtcgccctg
    27001 cgtcaggggc acgtcttcta cgacggcagc agccaactgt ttgataacga acgttttgac
    27061 catctctacc gcagcattaa ccgcatcgaa gagaacgcga aagctgcctg atatccccat
    27121 cattgaggaa aacgaatgaa cgctaagata attgcctcgc tggccttcac cagcatgttc
    27181 agcctcagca ccctgttaac cccggcacac gccgaagagc aggaaaaggc gctgaatttc
    27241 ggcattattt caacggaatc acagcaaaac ctgaaaccgc aatggacgcc gtttttacag
    27301 gatatggaga agaaactggg cgtgaaggtc aacgccttct ttgccccgga ctacgcgggc
    27361 attatccagg ggatgcgctt caataaagtg gatattgcct ggtacggcaa cctgtcggcg
    27421 atggaagcgg tggatcgcgc caatggccag gtcttcgccc agacggtcgc ggcggatgga
    27481 tcgccgggtt actggagcgt gttgatcgtc aacaaagaca gcccgatcaa caacctgaac
    27541 gatctgctgg cgaagcggaa agatctcacc ttcggcaatg gcgatcctaa ctccacctct
    27601 ggcttcctcg tccccggtta ctacgtcttc gctaaaaaca atatctccgc cagcgacttc
    27661 aagcgcaccg tcaacgccgg gcatgaaacc aacgcgctgg ccgtcgccaa caagcaagtg
    27721 gatgttgcca ccaacaacac cgaaaacctc gacaagctga aaacctccgc gccggagaag
    27781 cttaaagagc tgaaggtgat ctggaaatcg ccgctgatcc taggcgatcc gatcgtctgg
    27841 cgcaagaatc tgtccgaaac taccaaagac aagatctacg acttctttat gaactacgga
    27901 aaaacgccgg aagagaaagc ggtgctggaa cgcctgggct gggcgccgtt ccgcgcttcc
    27961 agcgacctgc aactggtgcc gattcgccag ctcgcactgt ttaaagagat acaaagcgtg
    28021 aaaggcaata aagggctgaa tgagcaggac aagctggcga aaaccaccga gattcaggcg
    28081 cagctggatg acctggaccg cctgaacaac gcgttaagcg cgatgagttc ggtgagtaaa
    28141 gcggtgcagt aaatcgtagg tcggataaga cgccccggcg tcgcatccga caatgtgtag
    28201 gcgttgatgc cggatgcggt gcaagcacct tatccggcct acagaccgga gccaaacatg
    28261 caaaccatca ccatcgcccc acccaagcgt agctggttct cgcttctgag ctgggccgtt
    28321 gttctcgacg tgctggtcgt ctcgtggcag ggcgcggaaa tggccccgct tacgctgatt
    28381 aaagacggcg gcaacatggc aaccttcgcg gtaatgactc caacttactg atagtgtttt
    28441 atgttcagat aatgcccgat gaccttgtca tgcagctcca ccgattttga gaacgacagt
    28501 gacttccgt
//
LOCUS       DAEBXN010000048        28365 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.048, whole genome shotgun sequence.
ACCESSION   DAEBXN010000048 DAEBXN010000000
VERSION     DAEBXN010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28365
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.048"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(41..1036)
                     /gene="yjfF"
                     /locus_tag="KJL99_002119"
                     /pseudo
     CDS             complement(41..1036)
                     /gene="yjfF"
                     /locus_tag="KJL99_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
     gene            complement(1023..2045)
                     /locus_tag="KJL99_002120"
     CDS             complement(1023..2045)
                     /locus_tag="KJL99_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBD4436843.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGITLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEVVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(2059..3561)
                     /locus_tag="KJL99_002121"
     CDS             complement(2059..3561)
                     /locus_tag="KJL99_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBD4436844.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEMELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDELTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(3701..4657)
                     /gene="ytfQ"
                     /locus_tag="KJL99_002122"
                     /pseudo
     CDS             complement(3701..4657)
                     /gene="ytfQ"
                     /locus_tag="KJL99_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
     gene            4967..5497
                     /gene="ppa"
                     /locus_tag="KJL99_002123"
     CDS             4967..5497
                     /gene="ppa"
                     /locus_tag="KJL99_002123"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HBD4436845.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVAVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(5877..6218)
                     /locus_tag="KJL99_002124"
     CDS             complement(5877..6218)
                     /locus_tag="KJL99_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HBD4436846.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(6221..10000)
                     /gene="tamB"
                     /locus_tag="KJL99_002125"
     CDS             complement(6221..10000)
                     /gene="tamB"
                     /locus_tag="KJL99_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HBD4436847.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQLGVAVKAGNLHLGVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVVEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTLGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(9997..11730)
                     /gene="tamA"
                     /locus_tag="KJL99_002126"
     CDS             complement(9997..11730)
                     /gene="tamA"
                     /locus_tag="KJL99_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HBD4436848.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQHVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFVGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            11936..12574
                     /gene="msrA"
                     /locus_tag="KJL99_002127"
     CDS             11936..12574
                     /gene="msrA"
                     /locus_tag="KJL99_002127"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBD4436849.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            12897..14240
                     /locus_tag="KJL99_002128"
     CDS             12897..14240
                     /locus_tag="KJL99_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBD4436850.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(14302..14508)
                     /locus_tag="KJL99_002129"
     CDS             complement(14302..14508)
                     /locus_tag="KJL99_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HBD4436851.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDIGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            14833..15387
                     /locus_tag="KJL99_002130"
     CDS             14833..15387
                     /locus_tag="KJL99_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HBD4436852.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFEIGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMDLLQKLLK"
     gene            complement(15450..16388)
                     /locus_tag="KJL99_002131"
     CDS             complement(15450..16388)
                     /locus_tag="KJL99_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4436853.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSHTKTHSNDFFNNIQHAFKKKDWV
                     SNYDSLLTLRESFRCATQIDKNSYQVLSSKNETVNAMDNFLISFSLKDNGAEYTITLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIRQ
                     AFGDVSFGYALLEDVVSKLKRGKFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     NITKEKICEKYKSTIEAFKKEQLALPVLVRCKNSET"
     gene            complement(16600..17340)
                     /gene="cysQ"
                     /locus_tag="KJL99_002132"
     CDS             complement(16600..17340)
                     /gene="cysQ"
                     /locus_tag="KJL99_002132"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HBD4436854.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLAPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKLIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            17530..19473
                     /gene="cpdB"
                     /locus_tag="KJL99_002133"
     CDS             17530..19473
                     /gene="cpdB"
                     /locus_tag="KJL99_002133"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HBD4436855.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VIVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIAPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(19599..19979)
                     /locus_tag="KJL99_002134"
     CDS             complement(19599..19979)
                     /locus_tag="KJL99_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBD4436856.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            20068..20928
                     /gene="qorB"
                     /locus_tag="KJL99_002135"
     CDS             20068..20928
                     /gene="qorB"
                     /locus_tag="KJL99_002135"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HBD4436857.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYSDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYATAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGCPTTTLAE
                     SVSHLFNVNN"
     gene            21037..22002
                     /locus_tag="KJL99_002136"
     CDS             21037..22002
                     /locus_tag="KJL99_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBD4436858.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            22110..22772
                     /gene="ytfE"
                     /locus_tag="KJL99_002137"
     CDS             22110..22772
                     /gene="ytfE"
                     /locus_tag="KJL99_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HBD4436859.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(22817..24229)
                     /gene="cycA"
                     /locus_tag="KJL99_002138"
                     /gene_synonym="dagA"
     CDS             complement(22817..24229)
                     /gene="cycA"
                     /locus_tag="KJL99_002138"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HBD4436860.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHVQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(24537..25157)
                     /gene="fklB"
                     /locus_tag="KJL99_002139"
     CDS             complement(24537..25157)
                     /gene="fklB"
                     /locus_tag="KJL99_002139"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBD4436861.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            25376..25921
                     /locus_tag="KJL99_002140"
     CDS             25376..25921
                     /locus_tag="KJL99_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OapA family protein"
                     /protein_id="HBD4436862.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNASVVTREAQLDIQSQSQPPTEEQPQTTQTQPFQPDSGIDNQWRSYRV
                     EPGKTMAQLFRDHGLPATDVYAMAQVEGAGKPLSNLQNGQMVKIRQNASGVVTGLTID
                     TGNNQQVLFTRQPDGSFIRAR"
     gene            complement(25905..26552)
                     /locus_tag="KJL99_002141"
     CDS             complement(25905..26552)
                     /locus_tag="KJL99_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBD4436863.1"
                     /translation="MYIDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYIANP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKYNIFKPGIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            26764..27555
                     /locus_tag="KJL99_002142"
     CDS             26764..27555
                     /locus_tag="KJL99_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HBD4436864.1"
                     /translation="MKSTRPVAVITGATRGIGKGCAQELARGGFNLLINDRPDADSVE
                     KLHITQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            27565..>28365
                     /locus_tag="KJL99_002143"
     CDS             27565..>28365
                     /locus_tag="KJL99_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBD4436865.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQITRQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLMPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDINS"
BASE COUNT         6960 a         7552 c         6992 g         6861 t
ORIGIN      
        1 tccttaactg atcggcatta ctccacacgg agaggcattt ttatcgctgc gcaatgttga
       61 ctcttgtcac tggcgaactc tgacgattct cccacagcac cgttaaccca cgctgtaatg
      121 ctataaaaat aaacaacaaa ataccgatgg cgattttcgt ccactaggaa ctcagcgtgc
      181 cgtcaaagtt gatgtaagtc tggatcagtc cctgaattgc cacgccaaaa agcgtcccaa
      241 gcaccgtccc gacgccacca cttaacaacg taccgccaat caccaccgac gcgatggcgt
      301 ccagttccac acctacgccc gccagtgcat atccggcttg ggtataaatc gagaagacaa
      361 tccccgccag tgttgccagt ccggtggaga gcatataaat gcgaatagtg gtgctgcgag
      421 tagaaatccc catcaggttc gccgacgtcg cgttgccgcc aatagcgtac acctgattgc
      481 caaaacgggt acaatgcgca aggaaaatac cgataaccac caccgccagc atcaacagcc
      541 ccatcgcact aaggcgaccg ccgccgggga ttttccacgc aaggcttgag agcgtgtcat
      601 aaatgggatg gtttatcgga atcgactctt ccgaaacgag atagctgacg ccgcgcaaaa
      661 agaacatccc cgcaagcgta atgataaatg ccgggatctt cagggcgtcg atcagaagcc
      721 ccataaatgc gccgaaggca cagcccatca ccagcactag cggaaacgcc agcagcggcg
      781 agaggccgaa atcgccaacc acttttgcca gaaacacgcc agtaaaggcg atcaccgaac
      841 cgacggagag atcgataccg ccagagagga tcacaaaggt catgccaacg gcgattatcc
      901 caagaaaggc gttatcggtc aggatattgc agatcaccct tgtggaagca aaaccgggaa
      961 actgggtcag gcagtagaga tagcccagca caaagacgcc gatggtgatc atcagcggca
     1021 agttacgttt tatcatggct acgtactcct ttaatcagac tgataaagcg ttgcgactga
     1081 acaatcagca cacaaagcac caccaccgct ttcaccacct ggttcatctc cggcggaaag
     1141 cccgaaagca aaattccggt gttcatcccc tgaataatca gcgcccccac taccgagagc
     1201 agcaggttaa agcgcccgcc catcagcgat ccgccgccaa tcaccaccgc gagaatggcg
     1261 tccagctcca gccataatcc ggcgttgttg gcatcggcac cgcgaatatc cgccgccacg
     1321 ataatgcccg caatcgccgc acatagcccg ctcaacacgt aggtgagcat gacgatgatt
     1381 cgcgtgttta ccccggcatt ttttgccgcc cgaatgttga taccaacgac ttcgataaac
     1441 atccccagcg ccgttttgcg ggtcaacagc cagaacagga gaagcgtcag caccgcgata
     1501 atgaccggcg ttggcaggaa caacagcgat ccgctgccaa accatgagag atccggcgag
     1561 ttaaacgtga cgatctgccc ggcggtgatc agttgcgcca cgccgcgccc ggcgaccatc
     1621 aggatcaggg tggcgacaaa cggctgaatt ttgaggatcg ctaccagtat gccgttccac
     1681 aatcccgcca ggatgccggt gcccagggcg cttaacaaaa caatcggcag gctgaatccc
     1741 gcgaccgtca tcgcagccgt tgtggctccg gcgatagcca tcaccgcccc cacagagaga
     1801 tcgataccac cggtggcgat caccagcgta ataccaatcg ccagtaacgc aacgggagct
     1861 gcacggttaa gaatgtcgat agggctaccg aacaaacgcc catcctgaag caccacctgc
     1921 cagaaatgcg gggctaccag gctatcgacc agcagcacca gcaacagtgc cgccagctgc
     1981 ggcatccccg tcggccagtg aaaacgcctt tttggcggcg tagtgtcagg gagagattga
     2041 ggcatcacac tgttctcctt acgccgcaat ggcgttcatg atcgccggaa cggaaagctg
     2101 tgccagcggg atctccgcca cctgtttgcg atcgcgcatg atgatcaccc ggtcggcata
     2161 gcccaccagc tcttccagtt cggaggagat caccagcagc gccagaccgt cagcacacag
     2221 cgtttcaatc aggcggatga tctcggcgtg ggcaccaaca tcaatgccac gggttagctc
     2281 atcaaggatc agaaattgcg gtcgggtcag tagccaacgt gaaagcagca ctttttgctg
     2341 attgccaccg gagagaaatt caatcggttg ttcagttgaa ggtgtgcgaa tgccaagctg
     2401 gcggataaag cgttcggcaa tctcttgctg ctctttgcgg gaaatgggtc gtagccagcc
     2461 gcgctgggcc tggagagcga ggatgatatt ttcccgcacc gaggcggcgg cgatgatgcc
     2521 atcggttttc ctgtcttccg ggcagaatcc gatgcccagt actgaagcct gatgtggcga
     2581 tcgcagggtt tgcggtttgc ctttgatcaa cgccgtgccg ctgtcagcag gtttgatacc
     2641 gaagatcact tcggcggttt cggtacgtcc ggatcccagc aagccagcca gaccgacgat
     2701 ctcgccgggg cgcacttcga gatcaaacgg tgctatcgtt ccttttttgc cgtaattttt
     2761 gaacgcggca acgggtttgt cgctcaacaa tgttcgcccg gcacgctgta gcgcgtgggt
     2821 atccagctcg cgccccagca tcatttttac cagttcgatc tgcggtagct cgcgcgtttc
     2881 ccgacagcct acgaaactgc cgttgcgtaa gacggtgatc cgatcgctga cctgatagac
     2941 ctgatcgaga aagtgagtga caaagatcag gctgacgccg cgatcgcgca actgacgcat
     3001 caggccaaat aatagctcca tttcctgggt gtcgaggctg gcggtgggtt catcgaggat
     3061 cagcactttg gcggagagat cgatagcccg gcaaatggcg acgatttgct gcatcgccac
     3121 cgaaaaacgg ttaagcggtt cgcgcacgtc gagggagaaa ccgtaagatg ccatcagttc
     3181 ggtggcgcgc ttttccatct ctttacggcg tagaaggccg aaacgtttgg gttcgcgacc
     3241 tatgaataga ttatcagcaa ccgacatatt gggtagcagg ttgacttcct gatagacggt
     3301 gccgataccg agttgttgtg catgggcggt atttttcggt gagatagtct ggccttccag
     3361 ccagatggtg ccgcgatcgg cgtggtatac accagttaat gctttgatta gcgttgattt
     3421 tcccgccccg ttttcaccaa gcagcgccat gatttcgcca cggcgcaggc tgaaatcaac
     3481 gttgtctaac gctttgacgc cggggaaaaa tttacttaat ccttcggtgc ggaggatctc
     3541 ctggtgttgg tcggtcgtca tggtttcccc cggtgtaaag cgtgctccca gtaggcatga
     3601 taagacgcgg taagcgtcgc atcaggcttc agtgcaccat cgccggatgc ggcgcgagcg
     3661 ccttatccgg cctacgaatc ggcatcaggc atagcaaccc tcaatacccc atatttttct
     3721 tcttctctaa ctcttctttt gcggtatcag gcaggtaaag ggtggatttg gttaacgtca
     3781 gcttttcagg catggtgccg tcttttttgt atttctccag cgcatcgaaa gcgggtcccg
     3841 ccatgttcgg cgtcagttca acactggcgt tcgcttcgcc atccatcatc gctttgtaga
     3901 tgtccggtac accgtcaatg gaacccgtga ggatatcttt gcccggtttc aggcccgctt
     3961 ctttaattgc ctgaattgca ccaatcacca tgtcgtcgtt atgggcgtaa accatgcaga
     4021 tgtttttgcc gttgttttcc gctttgataa agctctccat gacttctttg cctttactgc
     4081 gggtgaagtc acctgactgc gagcggataa ttttgatatt tggcgcattc ttaatggctt
     4141 cggcaaagcc tttcttacgg tcaatggcga cgctggcccc aacggtgccc tgcagctcca
     4201 ccacgttgca tggtttgcca ttcacttctt ttaccagcca gtcaccgatc aacttacctt
     4261 cgaggatgtt gtcggcagta acggtggtca tatagagaga tttgtctttc acatcgatgg
     4321 aacggtcgag caagaagacc gggatttcgg catctttcgc ctcttttaat accggttccc
     4381 aacccgtcgc aaccactgga gcgataaaga tcgcatccac cccttatgca acgaaggaac
     4441 gtaccgcttt aatctggttt tcctgctttt gctgaccatc ggcaattttc aacgtaattc
     4501 cgcgtttttc ggcctcactt ttcgccacat tggtttctgc ggcgcgccag ccggattccg
     4561 atccgacctg cgaaaatcct acggttaatg gagcggccaa cgccatagac gacatggctg
     4621 ccgagactgc agagactaca agtaagcgtt tccacatagg gttgtcctcg tcggggtaat
     4681 tattattggt gaaaagatgt tcgcgaaaaa actatagaca attcgttatg taacggattg
     4741 cgttacatca cacttcaaaa aagtaaataa aacgttaatc acaagtttgt aatcgctttc
     4801 atctcactat gaaaaatgcg gctacggtta tggattttcc tgctctgtat accgtcttaa
     4861 aactggcgaa aaaggaaaat gaagacgaaa acaagcaaag acattcggcg cgagttggct
     4921 ataatacttg gcacttgttt gccacatatt tttaaaggaa acagacatga gcttactcaa
     4981 cgtccctgcg ggtaaagatc tgccggaaga catctacgtt gttattgaga tcccggctaa
     5041 cgcagatccg atcaaatacg aaatcgacaa agagagcggc gcactgttcg ttgaccgctt
     5101 catgtccacc gcgatgttct atccgtgcaa ctacggttac atcaaccaca ccctgtctct
     5161 ggacggtgac ccggttgacg tactggtccc gactccgtac ccgctgcagc ctggttctgt
     5221 gatccgttgc cgtccggttg ccgttctgaa aatgaccgac gaagccggtg aagatgcgaa
     5281 actggttgcg gttccgcaca gcaagctgag caaagaatac gatcacatta aagacgtaaa
     5341 cgatctgcct gaactgctga aagcgcaaat cgctcacttc ttcgagcact acaaagacct
     5401 cgaaaaaggc aagtgggtga aagttgaagg ttgggaaaac gcagaagccg ctaaagctga
     5461 aattgttgct tccttcgagc gcgcaaagaa taaataagtt cttctagcgc aataaccctg
     5521 aacgccgggc ttcggttagt aagggttttt ttatgcccgc gataaataaa ctctctattc
     5581 caccatcatt attctcagcg gttgcaaggc ttgaacggta agaacaagca aacccgacca
     5641 ccattttgct gttcatagcc acttgctgga agttagccga cctcactcat actcaccgtt
     5701 caaagcgtgc ccccgtttta cccctgcaca tttgtgaacg ccagcagagt tttttatgcg
     5761 attgcagata tggaaagcgg ctcggagaga aaagcaaaag atggaaaagt tacaaaaaaa
     5821 aatcgcgaga tacatcgcat aaaacaacgc cacccgaagg aggcgtgctt ttattattac
     5881 ttatccctgt ctaaccagtc gccgctttca attagcttta atccatcgac gggtcgttga
     5941 taaacgtaca tccatgcact cccatacggc gtctgaatca actggcgcgc gtattcaccg
     6001 cccctggtgc gcaaggcatc aagttcggcc agcgtggcgt tgtcaatacg ataaacttca
     6061 ccgtgtaccg ttccgttccc cggaactgcg cctggatagt ggcccaggct atacaactgg
     6121 tagttatcga tactgaaatc gcccagtaac tgggcattgg tcatccagtg actgttgcct
     6181 tgtttgtggc gtaaactgcc gtagacaaat attcgcattg ctaaaactcg aactgataga
     6241 gcaaatccag tgcctggtct acaccagaca cggcttccag atatagctta ggcatcaggc
     6301 gataacgtaa cgtgagtgtt gctatagagt caaatatacc cacgccgtat ttcacttgca
     6361 gacctggcaa tacatagccg ctgaccacta cctgggagga gtcgcctact cccagggtgt
     6421 cgagcgctaa attgcttacg ccaaacgtct cgccgatttt acccacaatc tggccacttt
     6481 gcgcaacccc cagaccaatc agcatcgagg tcattgccgc actgtcgctc tgatcgctct
     6541 ccagcccttg tccacgtagc aaataagaca atgcagcttg ttgcgacatc gccgggtcag
     6601 agaagatctc cgctttcggt tcgtccgcca gaccagtgac gcgaactccg gcgattacat
     6661 cgtcttctgt agcatccggg ttacgaatag cttcaatatt aagatacggt tgatccggcg
     6721 gaccagagaa cagtaactca cccttacgca caatcagatc ctgaccatag gcatggaagc
     6781 gcccttcagg gatgttgatc tgcccgttca ggcccagccc ttgtttgtcc tgtacgacgt
     6841 taagatcgcc cgtcagccgc gctttcaggc caaaggcgtc aatgcgcaca ttgttgccaa
     6901 cgtggacaat caggttactg ttaatcggaa tcgacgccgt tttcggctct tccggttgca
     6961 ggttatcgtt aagcatcacc acatcgctgg agacgcctac tgcgctttcc ggcagatcgt
     7021 gcaccacgat gcgcgcccac gggacatcca cgcgaccatc gagggtaaac aggtttggtg
     7081 tagcctcgaa tacaacatct ggcgatacat ccattcgtac catcggcggc acggtgatcc
     7141 gcactttact gcctttcgcc gttactcgtg cccgccagtt ttcaatttgg ctccagtcgg
     7201 catcaccgtt caggtagatt tcaccctgct gggtccgtac tgtaccggca agcgtcgagc
     7261 gcataccgtt aaagttgacc gcaagctggc tcggctgcat atcaaacggc ataaagttgc
     7321 cgtcgatatc cacacccgta acctgaagct gaccaaacaa ctgcgggctt tgcacatcac
     7381 cacccagacg caagttggca ctcaccatcc ccgctgcttt ttccccacgg gtaaagatgg
     7441 ggtttatcat cgcaaggttg aagttacgga tattgacgtt gccaccaaga ttacggcggc
     7501 cttgcggatc ggtcacctgc acctgtccat caaactggcc gttattggtc aggcggatgg
     7561 tccagcccaa ttcggcacgg ttgttacgca attccgccgt cagattcagt gtctgaaacg
     7621 ccaccggcag cgccgcatcg ttgacggttt gcgttacctg cacgttacgc cccgaaaggg
     7681 tgatactgcc ctgcggcagc ccctctttcg tggtgtccca ggcaacatcc gctttacccg
     7741 tgaagatacc gctggcctga gtggtttctg gcataaacgg tttcagcatg gcgaggtcga
     7801 agcggttgag attcaccacc gcacgccctt cggcccccgc atcgatagtt tgcggcacgc
     7861 acagttccgc attcgggtta agccagcagt gtggcccgat gctgattttt tgctccttat
     7921 tgcggtaatc cagcgcaata tcgcgggtca gcgaccacgg gccgaccggc gtctggaagc
     7981 gggtattgct aagagttccc ttccagcgtt cttctttgcg atcaaaactt cctgccagat
     8041 taagctgccc ggagacaggc tcgccctgaa tccgcaactg tagctcgtgc tgcttttcgc
     8101 tgcctttggc attcagggtg acgaggttga tatttacatc cggctgcgaa atttgctcaa
     8161 cgcgtacgtc gagtttcccg gcgatctgat cggtggattt aatgtcgcct tccacgcgaa
     8221 cctgcgccac ggaaagttcc tgccagcgca ggccgcgcgc ggtgatatct gccagtagtt
     8281 gtggcgcttc caccgtgccg cgtactttca ccagcccttt cgctgtaccg ccaagccccg
     8341 gcagcgcgtt atccagcccc ggcgcgttga tggtggcatc aagattgaga tcttttaccc
     8401 ccagctcgcc tttcacttcg gcactgtttg gcccgagttc cagatgaagc ccagggatca
     8461 tccactgcat ataactgttg cctttcagcg tgccgtcaac gttcactttg ttctgtttga
     8521 cgttaccggt cagcttcagt tctggcacct ccatctgcca ggtgccgccg tacaggctac
     8581 cgcgggtttt aatcaagcca ttgagtttcg acggccactc cgggaactct ttggcggtgt
     8641 taatgccatt aagcgttagc tcaccgcgcc aactaatggc ctgctgccag tcgagcaacg
     8701 ccttgagttc agttttccct tccagcgccg cgacggtgag tttgtcgaga ttgacctgct
     8761 gttcattacc tttggcatca agggtaatgg tggctggcgg gatctcctgt cccttcactg
     8821 ccgtacgcat agagagcgtg taatcggtca ttttgccggt aagtttcagt ttcagatcat
     8881 ccgcctgata ctgcttctca ccagtgaacg gccagtaaag ctgtttgctg ttcacttcca
     8941 cgttgagtgg caatccggct tccgccagtc gcgtctgggc gcgtaaatcc atatccaccg
     9001 gaccggaaag gttaacgcct atctccagct gttcgcgcag cgcgccgccc actttcagct
     9061 tcaccttttc acctttcaac ggctccacgt tcagtgtgct gttgagggtg atatccaccg
     9121 gccagttgtc tgacagctgc gccgtgccgc tggcgttgac gatcccctgg ttggaatcga
     9181 tatccagggc gtccagttta gtattgccgt caatgctgct cactttcagc agcatggtgc
     9241 gcacggtgat gtccgtgtcg cccgtcacgc gcagctgctc gcccttaaac tcttcaatgt
     9301 tcaggttaag cggcaaatgc acgtcggtca tttccggcaa taccgggcga gaaaagagat
     9361 ctttcagcgt ttcgccgagc ggcttttcct ccggctgcgg attttcaatt ttcggttcga
     9421 ccacttcttc ctgcgccact tccaccactt tcggcagagc aatcagcagg cctttcagcg
     9481 acgtcggttt cagggtcagg gttttctcct gccagttcag gccggaggtg aagtccatca
     9541 ccgacaccgt ggtgtcatca atcttgatgt tgacgttgtc cagcgccacc cgtgtcaggg
     9601 tgatgggata cggcgtggag agatccagcg gaccgctatc ttcctcttct tcaacctgtt
     9661 cagaaggagg cattttttta ctgtcgatgt tgacctgaat gtctttcagc gccaggtcat
     9721 taatacaaac gctactgttc cacaggcact caagaccgac ccccagatgc agattacccg
     9781 cttttaccgc cacgcctagc tgctcataac gaacgtcaga caaggtgaga tcgcgccagc
     9841 cgccggtgac cttgccaata tccagtcctg gcacccagcg atccgccgct ttaaatacaa
     9901 gatgcaggcc gctggtggtg cccaccagaa acgccaccga tcccagcaac agtaagataa
     9961 cgataaccac gccgaggctg atttttttcc ataaactcat aattctggcc ccagaccgat
    10021 gtaaaactgt aacccgtgtt cgtctttatc cgcgaccggt acggcaaaat cgagtttgat
    10081 tggcccgacc ggcgattccc agcgtacgcc gaccccggta ccggttttaa agtcgctgcg
    10141 gcgaatatcg cttaccgctt cgccactatc gacaaacacc gcgccccacc attttccggt
    10201 cacgttgtac tggtattcca gcgatccggt tatcaacttc gaggcccctt tcaggtcacc
    10261 gttggcgtat ttcggggcga tagatttgta tttgtagccg cgaatactgc gatcgccccc
    10321 gacgaagaaa cgcagatccg gcggtacttt gtcgaaatca ccggtttcaa tccagcccag
    10381 cgtgccgcgt gtcacaaagc gatggcgatc gtacagtgtg cggatccaga cgtgctgcgc
    10441 ctggaaaacg gagaaatcga catctgaacc ccaggccgtg ttggagtagt cgatagagta
    10501 gcgttgcgag tctccccagg ttggcatcag gccaccacga gaacgcgtgc ggctaatcat
    10561 caccccagga taaaacagca tcgtggtgtt ggtaatttca ccctgggtaa agtggtcgag
    10621 gctccagcgc aggttaatgg cacgctgcca gccgctggag agatcccagt agcgagaagc
    10681 caccagcgta gtggagtcag attcggtatc gttcaggtca gtgcgcttaa aaccgccctg
    10741 caccaaataa tattgttcca gtggattctt cagcagcggc attttatagc tgaagtcgag
    10801 ggtctgttcc ggcgcggaaa tactggtact ggtggtcaaa ctgtgaccgt atgagttcat
    10861 ccacggcttt ttccacgtcg ctttcacgcg cggtcccacg tccgtagagt aaccgacccc
    10921 ggtttcgatg gtgttttctg ttcgcggcga aaccacgccc gtcaatggta atactttcgt
    10981 ttcgcgcgct ttatcaaatt gtggagccac caccaccgag ttaaaccagc cggtagcaga
    11041 aagtcggcgg ttcagttccg ccagatcttt cgattcatac tcatcgccct ctttaaacgg
    11101 caccagattt tgcaggtatt catcgcggat ttgtgatcct tcaaaggtca catgcccaaa
    11161 gcggtaacgt tcgccactgt tataatcaat atcccagaag gctttatgca ggccgagcgc
    11221 aatgcccagc tgcgctttgg taaattcgct atcgaaataa cctttacgca acgcaatact
    11281 ggttaaggac tttttgaaat tttcataatc gccctggttc agtaccgtac caatagccgg
    11341 gcgagtatcg agcaatttca aatagtcttt atcggtccgc gcgccgccgc gcaataccac
    11401 atcggtgccg ccaattaaca ccggcacacc tggcgtgact ttggcgatca atacctgccg
    11461 ccctttcttt ggcggtggac ggagatcaaa ttcaatggtc ggctggtaat aacccagcgc
    11521 tttcagacct tcacggatgg catcatcgac gcgtgcgcga aagcgacggt ctggcgtcac
    11581 ttcatcactt tcaatcgtag aaagctgcgc acgaacgttc ttttccagct gtcccgataa
    11641 cccctcgacc tgtagacgga cgttcgcggc gacggcagat ccgcttaagc agagtaagct
    11701 tacacagcat aactgtcgga tatagcgcac attttctcct gaatatcctt ttttcctgcc
    11761 cctggaacgc cgttaaacgg cttaacaaaa atccagtaat atggattaaa aagcagacta
    11821 aaccccaaat atttcttatg ttttacttta gacctattca cggtggttat tgtgtgcaaa
    11881 tacgcctctt gttacaacct taaccccaat gaccgatttt cgggagagcg acaccatgag
    11941 tttatttgat aaaaagcatc tggtttcccc cgccgatgcc ctgcctggac gtaacacccc
    12001 gatgcccgta gccacgctgc atgcggtcaa cggtcactca atgaccaatg tacctgacgg
    12061 aatggagatt gccatttttg cgatgggttg tttctggggt gtggagcgtc tgttctggca
    12121 gttacccggc gtttacagca ccgccgcagg ctataccggc ggctatacgc caaatccgac
    12181 ttatcgggaa gtgtgctccg gtgatacggg tcatgccgaa gcggtacgca ttgtttacga
    12241 tccttccgtc atcagctatg agcagttgct acaggtattt tgggagaatc acgatcccgc
    12301 ccagggaatg cgtcagggca atgaccacgg cacgcagtat cgttcagcga tttatccgct
    12361 gaccccagaa caggatgccg cagctcgcgc cagtctggaa cgttttcagg cggcgatgct
    12421 tgccgccgat gacgatcgtc acatcaccac ggaaatcgct aacgccacac cgttttatta
    12481 tgccgaagat gaccaccagc aatatctgca taaaaaccca tatggttatt gtggaattgg
    12541 cggaattggc gtctgtctgc cgccggaagc atagcgttac gggtacaaat gtagtttgtt
    12601 gataaagtgc tctttgtcta tgcctgatgc ggcttaaacg acttaccagg cctacaaaat
    12661 cgcccaaatt caatatattg caaggactgc gtaggcctga tcggcatagc gcatcaggca
    12721 gtattgcatt tatcagcagt ctgaatttta accctctggc gactttacag taccttacgc
    12781 tatactagcc actgaaaatg ccggttcact ttcttcgaat cggctttcaa tgtgtatttc
    12841 acacaaatta atcaactttc ccttccgagg gtctggcctg aaaggtcgga taagatatgt
    12901 taaacagtat tttagtcata ctctgcttga tcgctgtaag tgcgttcttc tcgatgtccg
    12961 agatctcgct tgccgcctca cgcaaaatca aacttaaact gctggctgat gaaggcaata
    13021 taaatgccca acgcgttctg aatatgcagg aaaatcccgg catgttcttt accgtggtcc
    13081 aaatcggtct gaacgcagtg gcgattctcg gcggtatcgt cggtgatgcg gcattttctc
    13141 cagcttttca cagcctgttc tcccgctata tgtcggcaga gctctctgag caactgagct
    13201 ttattctctc tttctcgtta gtgactggca tgtttatcct gtttgcggat ttaaccccga
    13261 aacgcatcgg tatgattgcg ccagaagcgg tggctttgcg tatcatcaac ccgatgcgct
    13321 tctgcctgta cgtttgcacc ccgctggtgt ggttcttcaa cggcctggcg aacatgatct
    13381 tccgtatttt caaactgcca atggtacgta aagatgacat cacttctgat gacatctacg
    13441 cggtagtgga agccggtgcg ctggcgggcg tgttacgtaa acaggaacac gagctgattg
    13501 aaaacgtctt tgagctggaa tcccgtaccg ttccgtcttc aatgacaccg cgtgaaaacg
    13561 tgatttggtt tgatctccac gaagatgagc aaagcctgaa gaataaggtg gcggaacatc
    13621 cgcactctaa gttcctcgtc tgtaatgaag atattgacca catcatcggt tatgtcgatt
    13681 ctaaagacct gctgaaccgc gtgctggcta accaaagcct ggcactgaac agcggcgtac
    13741 aaattcgcaa cacgctgatt gtgccggata cgttaaccct ttcagaggcg ttggaaagtt
    13801 ttaaaaccgc aggtgaagac ttcgcggtga tcatgaacga gtacgcgctg gtggtgggga
    13861 tcatcaccct caacgacgtg atgaccacgc tgatgggcga tctggtcggt caggggctgg
    13921 aagagcagat tgtcgcccgt gatgagaact catggctgat tgacggcggc accccaattg
    13981 acgacgtcat gcgcgtgctg gatattgacg agttcccgca gtcgggcaac tacgaaacca
    14041 tcggcggctt tatgatgttt atgctgcgta agatcccgaa acgcaccgat tcggtgaaat
    14101 tcgccggcta caaatttgaa gtggtggata tcgataacta ccgcatcgac cagctgctgg
    14161 tgacccggat cgacagcaag gccaccgccc tttcgccaaa actgcctgac gctaaagata
    14221 aagaagaaag cgtcgcgtaa cccccagaaa catcaacggc tcctgaatca ggagccgtct
    14281 tattactgca tagcactttg gttaagccat ctctgtttgc agacgcataa cctgacggtt
    14341 aacttcggac atcactgaca aatgcagctt atccttcact tttgggataa gaatcttacc
    14401 cttatcaaat tcaaaagcgc caatgtcctt gatgtataac cgtccacgga acaggatctt
    14461 tacgtacttc gccacctgaa gtgggttgta ccgttggaaa attttcattg ttatctcctg
    14521 ctgagtatta cgcctgtgcg gtgccacaat cggcccaact attatgaggc gcaaatttta
    14581 atgcttagtg actatagact atccgggcaa tgtttccacc gcgtataact ttttttacct
    14641 aaaggttaca attattcaga attatctttt taccgaagcg cggtcttcag tataagcatt
    14701 catttttcat atgatttgtg cgcttgaccg caaactggca tcacacttgc gggaaattcg
    14761 ataaatagca catatgatta aaactcagac ccaagtggtc ggatcacctg catatcataa
    14821 gaaggaaaca ccatgaccct acgcaagatt ctggcactca cctgcctgct gttgccgatg
    14881 atggcttccg cacatcagtt cgaaatcggt cagcgagtgc cgccgattgg cattaccgat
    14941 cggggcgagt tggtgcttga taaagatcag tttagctaca aaacctggaa cagcgcgcag
    15001 ttagtgggaa aagtgcgagt actgcaacat attgctggtc gcacctctgc aaaagagaaa
    15061 aacgcgacgc tgattgaagc gattaaatca gcgaagttac cgcacgatcg ttaccagacc
    15121 accaccattg ttaacaccga cgacgcgatt ccgggttccg gcatgtttgt gcgcagcagt
    15181 ctggagagta ataaaaagct ttatccctgg tcgcagttta ttgtcgatag caatggcgtc
    15241 gcgcgcggtg cctggcagct ggatgaagaa agttccgctg tagtggtgct ggataaagac
    15301 ggtcgcgtgc aatgggccaa agacggggcg ctcacaccgg aagaggtgca gcaagtgatg
    15361 gacctgctgc aaaagttgct caaataatgg caataacata aattctggaa aaataatttt
    15421 cccctatcgt tatctatggg ataggggaat taagtctctg agttcttaca ccgaactaat
    15481 actggtaacg ctagttgctc ttttttaaac gcctcaatgg tcgatttata tttctcgcaa
    15541 atcttttctt tggtaatatt actatctgcg gccagattcg cacttactat cgcatccact
    15601 tgttcggcta attcgacttg ttggcaacct gcattaacca gaaaattata aataagagag
    15661 taatcttcat cctccaaaac aacagaggaa ccattacgaa aacgttcatc ccctccatag
    15721 attttaagcg caaagcccaa agattctatg actatggcaa aattatcatc aattattttt
    15781 tcgtaaatat accatccatc gcattcttta attccaccgc ctggtatttg gagttcaaat
    15841 ttaccccgtt ttaatttgct tactacatcc tccaataagg cataaccgaa agatacgtca
    15901 ccaaacgcct ggcgaatata acctttaatc ctttttggta tgtcagactg ttttttctgc
    15961 aaaatatcac aggcatacaa tcttctgttt tgttctaacg gaaactcgcg gtttttgaaa
    16021 tccatgaatg agttatactc atttattgtt atgggaattt cttcgtattc aaacccactg
    16081 cctcgcaatg ttattgtata ttcagcaccg ttatctttaa ggctgaaact gataagaaaa
    16141 ttatccatgg cattaacagt ttcatttttt gatgaaagca cttgataact atttttatca
    16201 atctgtgttg cgcaacgaaa ggactcccgc aaagttaaca gactatcata attcgaaacc
    16261 caatcttttt tcttaaatgc atgctgaata ttattaaaaa aatcgtttga atgtgttttt
    16321 gtatgcgatg actttacaaa taactccacc gcaccaataa aattattgtg tctgatcctg
    16381 ggtaacatat ggctacctta ttttatttgg ttattttatg tcgccattaa atatctcatt
    16441 atagaagtaa actatcaaaa aacgctcatt attaaaatgt aacttactag caattgaatt
    16501 tttgcacttt aaatacaatc tctttttatt tataactttt taggttggga taaatcagga
    16561 tggcagaatc aggaaataca ctgtttctgc catctgaatt tagtaaatgg acactctgaa
    16621 ccccggattc aggaacgact cgcgcggagt gtaatccagc ggtttaccct gccagtcgtg
    16681 aacgtgcgct ccggcagctg cagccacagc atgtccggcg gcggtgtccc aaatattcgt
    16741 tggtccgaag cgcgggtaca gctgcgcctg tccttccgcc accaggcaga atttcagcga
    16801 agagccgatg gacgtggtct gatgttcgcc aagctgttgc agatactctt tcagctccgc
    16861 atctgcatgg gaacggctga taaccaccag cggcgggcgc gcatcgcgga cctgaatcag
    16921 cttgcgcaca ccgcactctt ctttccaggc cttgccttct gccgcgctgt acattacgtt
    16981 cattaccggc gcatacacca cacctaaaat cggtttgcca tggtcaatga gcgcaatgtt
    17041 aacggtgaat tcgccattac gtttaataaa ctctttagta ccatccagcg ggtctaccag
    17101 ccagtaacgc tgccagtgct gacggacttc ccaaccggga ggatcttctt cagaaaggac
    17161 cggaatatcc ggtgccagcg tacgtaaacc gtccatgata acggtgtgag cggcaatatc
    17221 cgctgccgtt accggagaat tgtccgcttt gctgacgacg tccatcggtt tcgtcccgtc
    17281 gtagacctgc ataatggcat cgcctgcatt ccgtgcaagc tggcatactt gatctaacat
    17341 ttctccacct cgtctctgtg agcggtgtta acttattgtt ttacttatac cctatcgtta
    17401 atgaatgcgc caactgtgat agtgtcatca ttttcaaagc gtaaaattgt ggcattcttc
    17461 actgttctat aagtaaggcg tttattctcc cttttctttc gtattcccga tgataaaagg
    17521 atgtccctga tgattaagtt tagcgcaacg ctcctggcca cgctgattgc cgccagtgtg
    17581 aatgcagcga cggtcgatct gcgtatcatg gaaaccactg atctgcatag caacatgatg
    17641 gatttcgatt attacaaaga caccgccacg gaaaaattcg gactggtacg tacggcaagc
    17701 ctgattaacg atgcccgcaa tgaagtgaaa aacagcgtac tggtcgataa cggcgatttg
    17761 attcagggga gtccgctggc cgattacatg tcggcgaaag gattaaaagc aggtgatgtt
    17821 catccggttt ataaggcgct gaatacgctg gattatacgg tcggtacact cggcaatcat
    17881 gaatttaact acggtctgga ttacctgaaa aatgcgctgg cgggagcgaa attcccttat
    17941 gtaaatgcca acgtcattga cgccagaacc aaacagccaa tgtttacacc gtatttaatt
    18001 aaagataccg aagtggtcga taaagacgga aaaaaacaga cgctgaagat tggctatatt
    18061 ggcgtcgtgc cgccgcaaat catgggctgg gataaagcta atttatccgg aaaagtgacg
    18121 gtgaatgata ttaccgaaac cgtgcgcaaa tacgtgcctg aaatgcgcga gaaaggtgcc
    18181 gatgtcattg tcgttctggc gcattccggg ctgtctgccg atccgtataa agtaatggcg
    18241 gaaaactcag tttattacct cagtgaaatt ccgggcgtta acgccattat gtttggccat
    18301 gctcacgccg ttttcccggg taaagatttt gctgatatcg aaggggctga tatcaccaaa
    18361 ggcacgctga atggtgttcc ggcggtaatg ccgggcatgt ggggcgatca tcttggggtg
    18421 gtcgacttac aactcagtaa tgacagcggt aaatggcagg tgacgcaggc gaaagcggaa
    18481 gcacggccga tttacgacat cgccaataaa aaatccctcg cggcggaaga cagcaagctg
    18541 gtagaaacac tcaaagccga tcacgatgcc acacgccagt tcgtcagcaa gccaatcggt
    18601 aaatctgccg acaatatgta tagctatctg gcgctggtgc aggacgatcc gaccgtgcaa
    18661 gtcgtgaaca acgcgcaaaa agcgtatgtc gaacattaca ttcagggcga tccggatctg
    18721 gcaaaactgc cggtgctttc agctgccgca ccgtttaaag ttggtggtcg caaaaatgac
    18781 ccggcaagct atgtggaggt ggaaaaaggc cagttgacct tccgtaatgc cgccgatctt
    18841 tatctctacc ccaatacgct gattgtagtg aaagccagcg gtaaagaggt gaaagagtgg
    18901 ctggagtgct ccgccggaca gtttaaccag attgctccta acagcacgaa accacagtca
    18961 ctcatcaact gggatggttt ccgcacttat aactttgatg ttattgatgg tgtgaattat
    19021 cagattgatg ttacccaacc cgcccgctat gacggcgagt gccagatgat taatgccaat
    19081 gcagagcgga ttaagaacct gacctttaat ggcaagccga ttgatccgaa cgctatgttc
    19141 ctggttgcca ccaataacta tcgcgcttac ggcggcaaat ttgccggtac gggcgacagc
    19201 catatcgctt ttgcttcacc ggatgagaac cgctcggtgc tggcagcgtg gattgctgat
    19261 gagtcgaaac gtgcggggga aattcatccg gcggcagata acaactggcg tttagcaccg
    19321 atagctgccg ataagaaact ggatatccgt ttcgaaactt ccccgtcaga taaagccgca
    19381 gcgtttatta aagagaaagg gcagtatccg atgaataaag tcgcgaccga tgatatcggg
    19441 tttgcgattt atcaggtgga tttgagtaag taaaacactt ctttttcggc ctataaatca
    19501 accgcatccg gcatttattg gcggatgcga tgctgccgta tcttatccac cctacaagcc
    19561 atgaaccgta gaccggataa gcgcagcgca tccggcagtt atgccgcacg ttcatcccgc
    19621 accgccaaca cctcgggcaa attcaactca atccagtccg ccagtgcagc aaccttttcg
    19681 ctcacctgct cgcccagcgg cgtgaggcta tattccacat gcggcggcac caccggatac
    19741 gcgatacggt taagaaaacc atcctgttct aacgcctgta acgactgcgc aagcatcttt
    19801 tcactcaccc caccgatttt gcgccgcagg tcgctaaagc gatgagtacc ttcgcgtagc
    19861 gccaccagaa tcaacacccc ccaacggctg gtgacgtgtt tcaacacctc gcgcgacggg
    19921 cactgttccg caaagagatt accctctttc agttgttgtg acaggctaac ctgactcatt
    19981 tcatacttac ctttttgcac gtacttacta aaagtaagtt taggtgttag cgtatttaaa
    20041 cacaagacaa aacgatggag acttcccatg atcgctatta ctggtgccac tggccaactt
    20101 ggtcactata ttattgaatc cttgatgaaa acggttcctg ccagccaaat agtggctatc
    20161 gttcgtaatc cggcaaaagc ccaggccctg gcagcacaag gcattaccgt gcgtcaggct
    20221 gactacagcg atgaagccgc actgacatct gcacttcagg gagtggaaaa actactgctg
    20281 atctcttcca gcgaagtggg tcaacgtgcc ccgcagcatc gtaatgttat taatgccgca
    20341 aaggcggctg gcgtgaaatt tatcgcttat accagcctgc tacatgcaga tacctccccg
    20401 ctcggcctcg ccgatgagca catcgagacg gagaaaatgc tggctgattc tggcatcgtt
    20461 tacaccctgc tgcgcaacgg ctggtacagc gaaaactacc tcgccagcgc cccggcagca
    20521 ctggaacacg gcgtatttat cggtgcggct ggcgatggca aaatcgcctc agcaacgcgg
    20581 gcagattatg cgacagctgc ggcacgcgtg attagcgaag ccggtcacga aggcaaggtt
    20641 tacgaactgg cgggcgatag tgcctggacg ttgacacagt tagcggcaga gctgaccaaa
    20701 cagagcggca aacaggttac ctatcaaaat ctgagcgaag ccgatttcgc cgcggcactg
    20761 aaaagcgtcg gactgcccga cggactggcg gatatgctgg cggattctga cgttggcgca
    20821 tcgaaaggtg gtctgtttga taacagcaaa acgcttagca aattgattgg ctgcccaacg
    20881 acaacgttag ccgaaagcgt aagccatctt tttaatgtta ataactagtt aattaaagtg
    20941 gcatcctccc gcatcctctc tgataatgac gggatgccgg gagcaatcat gtctgcttcc
    21001 tgaacgtttc ttctgacaga ccaatggatg ccagtaatga ttagcggcgt gctgtacgcc
    21061 ctgttagcag ggttgatgtg ggggcttatt tttgtcgggc cgttgatcgt gccggaatac
    21121 ccggcgatgt tgcagtcgat ggggcgttat ctggcgttag ggttaattgc gctacccatt
    21181 gcctggctgg gacgcgtgcg tctgcgtcag ttggcgcgtc gggactggct taccgccttg
    21241 atgctcacta tgatgggcaa cctcatttat tacttctgcc ttgccagtgc cattcaacgt
    21301 actggcgcgc ctgtttccac gatgattatc ggcaccctgc cggtggtgat tcctgtcttt
    21361 gccaatctgc tttatagcca gcgcgacggc aaactcgcgt ggggaaaact cgccccggca
    21421 ctgatttgta ttggcatcgg cctggcgagt gtgaatattg ctgagttaaa ccacggactc
    21481 cccgattttg actgggcacg ttatacctct ggcatcgtgc tagcgttagt ttccgtggtc
    21541 tgctgggcat ggtatgccct gcgcaacgcc cgctggctgc gggaaaatcc cgacaaacat
    21601 ccgatgatgt gggcgacggc gcaggcgctg gtcacgctgc cggtttctct catcggctat
    21661 ctcgtcgcct gttactggct gaatatacaa acgccggact tctccttacc ttttggcccc
    21721 cgtccgctgg tgtttattag tctgatggtt gcgatagccg tgctttgctc atgggttggc
    21781 gcactctgct ggaacgtcgc cagccagcga ttaccgacag tgattctcgg gccgctgatt
    21841 gttttcgaaa cgctggcagg tttgctgtac acctttttac tccgccagca aatgccgccg
    21901 ctaatgacgc tgagcggtat cgcgctgtta gtgattggcg tggtcatcgc ggtcagagca
    21961 aaaccagaaa aacctttaac tgaatctgtc tcagaaagtt gacatgctgg cagtgagtta
    22021 aataagcctc tgctacgtaa gggttatagc ttttgcctta aaggcgtatt taaaatacat
    22081 cttatcttat taagaatgag gtatcagcta tggcttatcg cgaccaacct ttaggtgaac
    22141 tggcgctctc tatccctcgc gcttcggctc tgtttcgtaa atatgatatg gattactgct
    22201 gtggcggtaa gcagacgctg gcgcgcgcgg cggcacgtaa agaactggat gttgaggtca
    22261 ttgaagctga actggcaaag cttgctgaac aaccgattga gaaagactgg cgtagcgccc
    22321 cgctggcaga aatcattgac catatcatcg tgcgctacca cgatcgtcac cgcgagcagc
    22381 tgccggagct gatcctgcaa gcgactaaag tcgagcgcgt tcacgccgac aaaccgagcg
    22441 tgccaaaagg gctgacaaaa tacctgacca tgctgcatga agagctttcc agccacatga
    22501 tgaaagaaga gcagatcctc ttcccgatga tcaaacaagg catgggcagc caggcaatgg
    22561 ggccaatcag cgtaatggaa agcgagcacg atgaagcggg cgaactgctg gaagtgatca
    22621 aacacactac caataacgtc acaccgccgc cagaagcctg caccacctgg aaagcgatgt
    22681 ataacggcat taatgaactg attgatgacc tgatggatca catcagtctg gaaaacaatg
    22741 tactgttccc acgcgcccta gcgggtgagt gatctaaaag ctggatggca ttgcgccatc
    22801 cagcatgata atgcggttat ttccgcagtt cagcagcccg cttcttacca ataaacagcc
    22861 agcccaaccc cagcgcgata aaccacagcg gggtaaccag cagcgcctgg cgagtgtcat
    22921 cttccagtgt cagcaacacg accacgaaca caaagaacgc catacatacc cagcacatca
    22981 gcttgccgag cggcatcttg tagatcgact tttcatgcag atgaggacgc tgtttgcggt
    23041 acaccaggta agagcaaagg ataatcgtcc agacgaacat aaacagaatc gcggaaacgg
    23101 ttgtaatcat cgtgaacgcg ccaatcacgc taggattcac atacaacatc accacgccgc
    23161 cgagcagaca gatacacgag aaggtcaacc ctttcgcggg tactgcgcgc ttagaaagtt
    23221 tagcgaacgc tttcggcgca acaccttcct gcgccagacc aaacagcata cggctggtag
    23281 agaagacacc gctgttagcg gaagacgccg cagaggtcag caccacaaag ttgatcacgc
    23341 tggcagccgc aggcaaacct accaacacaa acagctcaac gaacgggctt ttctccggga
    23401 ctaccgaact ccacggtgtc acggacataa tcacaatcag cgcgaagacg tagaacataa
    23461 tgatacggat cggaatggag ttaatcgcgc gtggcagtga tttctccgga tctttggttt
    23521 ccgcagctgt tgtacctacc agctcaatcc ccacgaaagc gaaaaccgct atctggaatc
    23581 cggcaaagaa gccacttaaa cctttcggga accagccgcc gtcattccac aaatgcgcga
    23641 atgacgcttc cacaccggtc ggtgactgaa agtgcatcgc caccatgacc aggccgacga
    23701 caatcagcga cacgatggcg acgattttga tcatcgcaaa ccagaactcc atctcaccga
    23761 acattttcac ggtggcaagg ttgagcgtca gcagcagcac tatcaccgcc agcgaggcga
    23821 cccagtcgga gagatcgggg aaccagaact gagcataagc cgtgatcgcc accacgtctg
    23881 ccataccggt tacaacccag cagaaccagt aagtccagcc ggtgaaatat cccgcccacg
    23941 gccccagtaa atcggaagcg aagtcactaa aagatttgta ttccagattc gaaagcagca
    24001 attcccccat tgcccgcatc acgaaaaaga gcataaaacc aatgatcata taaacgaaaa
    24061 tgatcgacgg cccggcaagg ctaatcgttt tgccggaccc cataaacaac ccagtaccaa
    24121 tggcacctcc aatggcaata agctgaacat gtcggtttgt gagattgcgc cgtagcgact
    24181 gttcagccgg agcctgatca tcggcaacga cttttacctg atctaccatg ttttttcttc
    24241 ctgtacctgt ctgtgttgtt caggctctac ggcctttagt cagtctatga tacgacgaat
    24301 ctgtatcggg actcatcgat attaggtaac aatacgcggg atgaatacta agatttagat
    24361 ctaatgttaa ttttatgttt aaagtgagtg tcatatcacg gtgataacgc gaaacagctc
    24421 acaaaaatac acgcttacgg ggtctttgca acacaaaata gcgccgtttg gcaaactgaa
    24481 gggtttattg ctgaatgcct gctcccctct ggttcgaggg gaacagaatg cgctgcttag
    24541 aggatttcca gcagttcgac ttcaaacacc agggtgctga acggagggat ggatgcacct
    24601 gcgccgcgct cgccatatgc cagttcctgc gggatagtca gttcccattt agaacctacc
    24661 ggcatcagag tcagtgcttc aatccagcca gggatcacgc cattaaccgg gaattcagcg
    24721 ggttcaccac gagcaacgga gctgtcaaac acggtgccgt cgatcagttt accggtgtaa
    24781 tgaacacgaa cgcggtcggt acgtgccgga attgcgcctt caccctggtt gatcacgcgg
    24841 aattgcaggc cagattcggt gctattcaca ccttcttttt tggcgttttc ttccaggtat
    24901 ttcacgcctt cagcagccat cgcctggaaa cgctgacgac gaacggcatc ggcgcgttcg
    24961 tggatttcac gcagcgcgcg atgcaccaca tcaaccggca cagccggatg tttgccttcc
    25021 agcgcatcgg caatacctgc aaccagtgct tctggcagca gcccttccag gccagattca
    25081 ctcagttgtt gccctacctg caaaccaatg ccgtagcttg cttgcgcttc gatggtgtca
    25141 aaagttgggg tggtcatcgt atttcctttc ttagggtata aaagtagcgg gcagcatatc
    25201 agccacactt ctttgggtaa aactttgtcg caggaatgat gacaaatccc ggggaaaaag
    25261 aaacaatagc aatatcccgt atgaatataa taaaggcggg tttaccacgg acatcatagc
    25321 gttagcttat tcataggcta tgattgagga acaagacgtg gagcaggagg aaaccatgcc
    25381 cgggcgcttt gaactaaaac caaccctgga gaaagtctgg cacgcgccgg acaattttcg
    25441 cttcatggac ccgctgccgc cgatgcatcg tcgggggatc attattgccg ccattgtatt
    25501 ggtggtcgga tttctgcttc catctgatga tacgcccaac gcgtcggtgg tgacgcgtga
    25561 agcgcagtta gatattcagt cgcaatcaca accgccgaca gaagaacagc cgcaaactac
    25621 ccaaacgcaa ccattccagc cagatagcgg aattgataat caatggcgct cttaccgtgt
    25681 cgaacccggt aaaacgatgg cgcagctgtt ccgcgaccac ggactgcccg ctaccgatgt
    25741 ctatgcaatg gcgcaggtag aaggcgcggg taagccgctg agtaatttgc aaaatgggca
    25801 gatggtgaaa atccgccaga acgccagcgg cgtggtgacg ggcttaacca ttgatacggg
    25861 gaataatcaa caggtcctgt ttacccgcca gccggatggc agttttattc gtgcgcggta
    25921 gatgaagggt caaccaaaag ccagctaagg attgtctctt tgatgacctt acgtctggct
    25981 tccagtgcct ggggggaaac catactgata tgataaacta ggccaagcgt atgctggttg
    26041 atcaaataat atcccccaag cgcagcaata ttaatattca cctgcaatgg gtcgatacct
    26101 ggcttaaaaa tattgtattt cttcccttca tccaataagg attccattaa ctgcaagtga
    26161 gcatggttaa tttctaagag acgctgtgat tttgcataat gcacaccttt actttgattt
    26221 tcactatgca caatttttaa aaaccaagga ttagcaatat aataatccca tgtaaaatca
    26281 ataattttct caatggcttt ttgtggcgga agatctgaga aatcaagggc cttttctttt
    26341 tgtcgaatat cgttccaggt atattccagc acttcgataa ataaattttc tttattacca
    26401 aagtggtgat aaaccgtctg tttactacac cctgctgcct ttacaatatt atctactctg
    26461 gccccttcat aaccatattc ggcaaattca ttaaccgcac atagcagcaa tttttccttc
    26521 aggctctgta cgttgctggt atcaatatac ataacgcttc cccacgacct cttcgtggtg
    26581 actatcataa cctgatttga tttcagcttc agaaaaaagg gcaaaaactt gtactttcat
    26641 caggttatca aatgtcctgt gatcgccatc gcatatcaac caactagatg gtttatcttt
    26701 ttatcctcca gtcgcaagct aatcatcacc aaggaaacaa aattcaaaaa agagagctat
    26761 tgtatgaaat ccactcgtcc tgttgccgtg attaccggag ccactcgcgg tattggaaag
    26821 ggctgtgcgc aggagcttgc tcgtggcggg tttaacctgc tgattaacga tcgtcccgat
    26881 gccgacagcg tagaaaaact gcatatcaca caacaagaat gtttggcgga aggcgtggaa
    26941 gtgatttgct tccctgctga tgtcggtgat ctatctctgc atgaggagat gctcgacgcg
    27001 gcgcaaaacc agtgggggcg gctggactgt ctgctcaata acgcggggat ttcagtgaaa
    27061 aaacgcggtg acctcctcga cctcgaacca gacagcttcg atcaaaacat tgccattaac
    27121 acccgcgcgc cgttcttcct tgctcaggca ttcagtaagc gcttactggc acagccgaaa
    27181 ccggaagcgg agctgccgca ccgctcgatt attttcgtca gttccatcaa cgccatcatg
    27241 ctggcgatga atcgcggcga atacaccatt gccaaaaccg ccgtttctgc cgctgctcgt
    27301 ctgtttgctg ctcgcctttg taatgaacaa attggcgttt atgaagtgcg ccctggtctc
    27361 atcaaaactg atatgactat tccggcaacg gcttattacg acgaactgat tgccaaaggt
    27421 ctggtgccgt ggggacgttg gggttatccg gctgatattg cttccaccgt ccgcgcgatg
    27481 gcggaaggta aactgattta cacctgtggt caggcggtcg caatcgacgg cggcttaagt
    27541 atgccgcgct tctgaggctg aatcatgaga gatctgttac aacgcccgga tttattcagt
    27601 atcaataccg cgacactggg ctataaaacg ccgctacccg ccattattga tgcctgcgcc
    27661 gcacgcggta ttggcgcaat tgccccctgg cgcagggagt tgcagggtga agatctccag
    27721 caaatcaccc gccagcttgc cgccagtaac atgagcgttt ccggtttgtg ccgtagcact
    27781 tactacaccg cgccgacact ggcggaacgc aaactggcga ttgatgataa ccgccgggcg
    27841 ctggacgacg cagcggtgct taacgccgcc tgttatatgc aggtggttgg cggtctgcca
    27901 acgggcacta aagatctata tgaagcgcga gaacaagtga aacaagggat tcgccagttg
    27961 ctgccacatt cgaaagatgt cggcgtgcca attgcgctgg agccactgca cccgatgaca
    28021 gctgccgatc gctcttgcct gtgcacgtta cgtcaggcgc tggactggtg tgatgaactc
    28081 gatcccgatg gtgagtttgg cctcggcgtg gcggtggatg tttatcacgt ctggtgggat
    28141 ccggatctcg ccagtcagat cctgcgtgcc ggaaaacgca tcctcgcgtt tcatgtttcc
    28201 gactggttaa tgccgaccac tgatctggtc aatgaccgtg gtatgccggg agatggcgtc
    28261 atcaatattc cgtcaattcg tcggctggtt gaaaacgccg gatttaatgg tgctattgaa
    28321 ctggaaatat tctcgcctta ctggtggcag aaagatatta acagc
//
LOCUS       DAEBXN010000049        27624 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.049, whole genome shotgun sequence.
ACCESSION   DAEBXN010000049 DAEBXN010000000
VERSION     DAEBXN010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27624
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.049"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(<1..908)
                     /locus_tag="KJL99_002144"
     CDS             complement(<1..908)
                     /locus_tag="KJL99_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraC family protein"
                     /protein_id="HBD4436866.1"
                     /translation="MSNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRL
                     LPYRDYNQESGLFMNDTTMGFMLEAIPINGANKSIVEALDHMLRTKLPRGIPLCIHLM
                     SSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMKAAATQFPLPEGMNLPLTLRHYR
                     VFISYCSPSKKKSRADILEMENLVKIIRASFHGAKITTQTVDAQAFIEIVGEMINHNP
                     DSLYPKRRQLDPYSDLNYQCVEDSFDLNVRADYLTLGLRENGRNSTARILNFHLARNP
                     EIAFLWNMADNYSNLLNPEMSISCPF"
     gene            complement(1034..1381)
                     /locus_tag="KJL99_002145"
     CDS             complement(1034..1381)
                     /locus_tag="KJL99_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4436867.1"
                     /translation="MKSIYLKSVLAFIFVGVMAMIVCIPFYIVYLAQQPATPEQLTEI
                     LQETPCAAEAFQETLNYQSEPLTLGKANKIASECRKRNEMAEVKRVRENERNKIREKQ
                     IQALNDAHSVKER"
     gene            complement(1409..1627)
                     /locus_tag="KJL99_002146"
     CDS             complement(1409..1627)
                     /locus_tag="KJL99_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4436868.1"
                     /translation="MHTFIFLVLIKNINGGGSWNITPMPNTDVCVQVLRTIEAHGGRG
                     FGNTGECIEVKTNEPFNSVMETLNGGGQ"
     gene            complement(1726..2199)
                     /locus_tag="KJL99_002147"
     CDS             complement(1726..2199)
                     /locus_tag="KJL99_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4436869.1"
                     /translation="MHKSEAEKIDSILDEHQQIYRRHNRIDNILAYCACIAWIPCIIA
                     AFGVDGIYLKILFAVLACCGAVCFFIFFFTLLPESLMVLSRQSLLQLMRLTEDVPDAR
                     QELLNRLLSGKKLTGRDEKDIRRLWQEKVDAMQESATRQREQDTIRKFAEESKVC"
     gene            complement(2192..2605)
                     /locus_tag="KJL99_002148"
     CDS             complement(2192..2605)
                     /locus_tag="KJL99_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4436870.1"
                     /translation="MHKSENEKIDFILDEHERISRKQGSLCMALGKGFLVIAILCGVA
                     TFCVSGLYLKSLCLSVACCCGVFSRIFNYYSVPEESRMVLSRQELLRLMRLTEDVPDV
                     RQELLNRLLSGKKLTGRDEQDIRRLWQETQRKHYA"
     gene            complement(2598..2819)
                     /gene="traR"
                     /locus_tag="KJL99_002149"
     CDS             complement(2598..2819)
                     /gene="traR"
                     /locus_tag="KJL99_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HBD4436871.1"
                     /translation="MSDEADEAYSVTEQLTMTGINRIRQEINVHGIPVYLCEACGNPI
                     PEARRKIFPGVTLCVECQAYQERQRKHYA"
     gene            complement(2954..3469)
                     /gene="traV"
                     /locus_tag="KJL99_002150"
     CDS             complement(2954..3469)
                     /gene="traV"
                     /locus_tag="KJL99_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HBD4436872.1"
                     /translation="MKQISLFIPLLGTLLLSGCAGTNTEFECNATTSDTCMTMEQANE
                     KAKKLEQSSEAKPVAASLPRLAEGNFRTMPVQTVTATTPSGSRPAVTAHPEQKLLAPR
                     PLFTAAREVKTVVPVSSVTPVTPPRPLRTGEQTAALWIAPYIDNQDVYHQPSSVFFVI
                     KPSAWGKPRIN"
     gene            complement(3466..3834)
                     /locus_tag="KJL99_002151"
     CDS             complement(3466..3834)
                     /locus_tag="KJL99_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HBD4436873.1"
                     /translation="MFISLMTEQTEATEIKMNMRNINVITARVVPDKNVSDDFMHAVL
                     SNCTTKIVLPAPKVFSSESLPHKFNMAAVGVMKKGMSMSLFSSPGNTVVSGHPCAGKT
                     NLTAELISAVVGSNKKGEGQ"
     gene            complement(3773..4363)
                     /locus_tag="KJL99_002152"
     CDS             complement(3773..4363)
                     /locus_tag="KJL99_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraP"
                     /protein_id="HBD4436874.1"
                     /translation="MANNMSSRQACHAARYVVARVLRGLFWCLKYTVILPLATMALMA
                     LFVLWKDNTTPGKLLVKEINFVRQTAPAGQFPVSECWFSSSDSSGRSEIQDICHYRAA
                     DAADYVRETDRSLMQLVTALWATLALMYVSLAAITGKYPVLPGKMKCIRVVTADEYLK
                     DVYTEDASLPGKIRKCHVYFPDDRTNRSNGDKNEHA"
     gene            complement(4353..5780)
                     /locus_tag="KJL99_002153"
     CDS             complement(4353..5780)
                     /locus_tag="KJL99_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraB"
                     /protein_id="HBD4436875.1"
                     /translation="MASINTIVKRKQYLWLGIVVVGAASAIGGVLYLSDVDMSGNGEA
                     VAEQEPVPDMTGVVDTTFDDKVRQHATTEMQVTAAQMQKQYEEIRRELDVLNKQRGDD
                     QRRIEKLGQDNAALAEQVKALGANPVTATGEPVPQMPASPPGPEGEPQPGNTPVSFPP
                     QGSVAVPPPTAFYPGNGVTPPPQVTYQSVPVPNRIQRKVFTRNEGKQGPSLPYIPSGS
                     FAKAMLIEGADANASVTGNESTVPMQLRITGLVEMPNSKTYDATGCFVGLEAWGDVSS
                     ERAIVRTRNISCLKDGKTIDMPIKGHVSFRGKNGIKGEVVMRNGKILGWAWGAGFVDG
                     IGQGMERASQPAVGLGATAAYGAGDVLKMGIGGGTSKAAQTLSDYYIKRAEQYHPVIP
                     IGAGNEVTVVFQDGFQLKTVEEMALERTQNRAEEDNPESPVPVPPSAESHLNGFNTDQ
                     MLKQLGNLNPQQFMSGSQGGGNDGK"
     gene            complement(5780..6508)
                     /gene="traK"
                     /locus_tag="KJL99_002154"
     CDS             complement(5780..6508)
                     /gene="traK"
                     /locus_tag="KJL99_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HBD4436876.1"
                     /translation="MRKNNTAIIFGGLFFSCSVMAANGTLAPTVVPMVNGGQASIAIS
                     NTSPNLFTVPGDRIIAVNSLDGALTNNEQTASGGVVVATVNKKPFTFILETERGLNLS
                     IQAVPREGAGRTIQLVSDLRGTGEEAGAWETSTPYESLLVTISQAVRGGKLPAGWYQV
                     PVTKETLQAPAGLSSVADAVWTGNHLKMVRFAVENKTLSALNIRESDFWQPGTRAVMF
                     SQPASQLLAGARMDVYVIRDGEGN"
     gene            complement(6495..7061)
                     /gene="traE"
                     /locus_tag="KJL99_002155"
     CDS             complement(6495..7061)
                     /gene="traE"
                     /locus_tag="KJL99_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HBD4436877.1"
                     /translation="MEHGARLSTSRVMAIAFIFMSVLIVLSLSVNLIQGVNNYRLQNE
                     QRTAVTPMGFNVPFAVSQNSADASYLQQMALSFIALRLNVSPEIVDASHQALLQYIRP
                     GAQNQMKVILAEEAKLIKKDNVNSAFFQTSVRVWPQYGRVEIRGIRKTWIGNSEPFTD
                     IKHYILILKRENGVTWLDNFGETDDEKK"
     gene            complement(7083..7394)
                     /gene="traL"
                     /locus_tag="KJL99_002156"
     CDS             complement(7083..7394)
                     /gene="traL"
                     /locus_tag="KJL99_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HBD4436878.1"
                     /translation="MSGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICVGWGITT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWI
                     K"
     gene            complement(7409..7768)
                     /gene="traA"
                     /locus_tag="KJL99_002157"
     CDS             complement(7409..7768)
                     /gene="traA"
                     /locus_tag="KJL99_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HBD4436879.1"
                     /translation="MNTVLSVQGASAPVENKSFFSKFTRLNMLRLVRAVIPVAVLMML
                     FPELAMAAGKGDLMAKGNDTVKATFGKDSSIVKWVVLAEVLVGAVMYMMTKNVKFLVG
                     FAIISVFIAVGMSVVGL"
     gene            complement(7802..8041)
                     /gene="traY"
                     /locus_tag="KJL99_002158"
     CDS             complement(7802..8041)
                     /gene="traY"
                     /locus_tag="KJL99_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome protein TraY"
                     /protein_id="HBD4436880.1"
                     /translation="MGGRVIKGALRSAPSRKVLLTLDEETNKLLLGARERSGRTKTNE
                     ALVRLQDHLQRYPDFYNEEIFREVIEENESTFKEL"
     gene            complement(8149..8796)
                     /locus_tag="KJL99_002159"
     CDS             complement(8149..8796)
                     /locus_tag="KJL99_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbJ"
                     /protein_id="HBD4436881.1"
                     /translation="MCAMDTRERALIRQSGLPEIILSAKYPVCVRTESGKFIDSNSVF
                     LHLIKCQNSDSEIWFSGIDIDTQILFHTAEVDAFSSSNGVSIINGVILNDVSWDVVVE
                     STEVNNLNLFVWRFFERYVVSGFFIKGKEFSCEPDKYRFNKIIPYLLGYSHEYSSQRM
                     DISVDQSKKIFMNFKKHYGFSDRDEWLRYAIISDVLFYLYQYFASNINSLHCKEM"
     gene            complement(8988..9371)
                     /locus_tag="KJL99_002160"
     CDS             complement(8988..9371)
                     /locus_tag="KJL99_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxosome protein TraM"
                     /protein_id="HBD4436882.1"
                     /translation="MAKVNLYISNDAYEKINAIIEKRRQEGAREKDVSFSATASMLLE
                     LGLRVYEAQMERKESAFNQAEFNKLLLECVVKTQSSVAKILGIESLSPHVSGNPKFEY
                     ANMVEDIREKVSAEMERFFPKNDDE"
     gene            9704..10294
                     /locus_tag="KJL99_002161"
     CDS             9704..10294
                     /locus_tag="KJL99_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020246007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HBD4436883.1"
                     /translation="MVRTTNLLSLFVEWVKLFTDKVTRGGKMKKWMLAICLMFINGIC
                     EAADCFDLAGRDYKIDPDLLRAISWKESRYRVNAIGINPVTGYGSGLMQVDSQHFNEL
                     ARYGIKPEHLTTDPCMNIYTGAYYLAIAFKKWGVSWEAVGAYNAGFRKTERQNQRRLA
                     YASEVYRIYTWIKSSKGIRVPTTKKSLSQINSVQKN"
     gene            complement(10591..11412)
                     /locus_tag="KJL99_002162"
     CDS             complement(10591..11412)
                     /locus_tag="KJL99_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HBD4436884.1"
                     /translation="MRLASRFGRYNSIRRERPLTDDELMQFVPSVFSGDKHESRSERY
                     TYIPTINIINKLRDEGFQPFFACQSRVRDLGRREYSKHMLRLRREGHINGKEVPEIIL
                     LNSHDGSSSYQMIPGIFRFVCTNGLVCGNNFGEIRVPHKGDIVGQVIEGAYEVLGVFD
                     KVTENMETMKEIHLNSDEQHLFGRAALMARYEDENKTPVTPEQIITPRRWEDKQNDLW
                     TTWQRVQENMIKGGLSGRSASGKNTRTRAITGIDGDIRINKALWMIAEQFREWKS"
     gene            complement(11523..11819)
                     /locus_tag="KJL99_002163"
     CDS             complement(11523..11819)
                     /locus_tag="KJL99_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4436885.1"
                     /translation="MSAFSFNTTYQPTGDQQKDAIAQIGIMQNRAVQANLAYQSCRDS
                     GALFKVLHQVNNELHDLLDSLENHTPLVRKNADELIALLLLFTRQVGQSRTDLI"
     gene            12119..12496
                     /locus_tag="KJL99_002164"
                     /pseudo
     CDS             12119..12496
                     /locus_tag="KJL99_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001451816.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(12734..12892)
                     /locus_tag="KJL99_002165"
     CDS             complement(12734..12892)
                     /locus_tag="KJL99_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HBD4436886.1"
                     /translation="MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAA
                     FMAYESGK"
     gene            complement(13172..13891)
                     /locus_tag="KJL99_002166"
     CDS             complement(13172..13891)
                     /locus_tag="KJL99_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HBD4436887.1"
                     /translation="MSARSQALVPLSAEQQAAWRAVAETEKRRHQGNTLAEYPYAGAF
                     FRCLNGSRRISLSDLRFFMPSLTAEELHGSRLQWLYAIDVLIETQGEVCLLPLPGDAA
                     ERLFPSVRFRVRERSRHKSALVMQKYSRQQAREAEQKARAYQALVAQAEIELAFHSPE
                     TVGSWYARWSDRVAEHDLETLFWQWGERFPSLAGMERWQWQDMPFWQVIAEASLAARE
                     AGHAVREMERWMVPNKLREAA"
     gene            complement(13888..14322)
                     /gene="psiB"
                     /locus_tag="KJL99_002167"
     CDS             complement(13888..14322)
                     /gene="psiB"
                     /locus_tag="KJL99_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HBD4436888.1"
                     /translation="MKTELTLNVLQTMSAQEYEDIRAAGSDERRELTHAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFAPEAVSHSLALAASLDAQGYSVSDIIHILMAEGGQA"
     gene            complement(14377..16335)
                     /locus_tag="KJL99_002168"
     CDS             complement(14377..16335)
                     /locus_tag="KJL99_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBD4436889.1"
                     /translation="MSVTESKTKTERKSSRKPAKTQETVLSALLEQTEEVSVSLASLI
                     KSPLNVRTVPYSAESVSELAESIKGVGLLQNLVVHALPGDRHGVAAGGRRLAALNMLA
                     ERGILPADWPVRVKVIPQELATAASMTENGQRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEFLREAEGWEWCAGRMEPVGECREDAGTYRCLPEPEAVLTEAEE
                     ERLNELMTRYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEMRAGGGVVVSWRYGNV
                     CVQRGVQLRSEDDVADDADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLTLLAWTLCLNVFGSGAYSKPAQISLECKHYSLTSDAPSGKEGAAFMALMAEKARL
                     AALLPEGWSRDMTTFLSLSQEVLLSLLSFCTACSLNGVQTRECGHTSRSPLDTLESAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNEAGLSGAARDAEKMKKGDAAEHAEHHMKD
                     NRWVPGWMCAPHPQTDTTERTDNLADAA"
     gene            complement(16399..16632)
                     /locus_tag="KJL99_002169"
     CDS             complement(16399..16632)
                     /locus_tag="KJL99_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HBD4436890.1"
                     /translation="MSEYFRIFQGLPDGSFTREQAEAVAAQYRNVFIEDDQGTHFRLV
                     VRNNSGLVWRTWNFEDGAGYWMNHVIRNFGILK"
     gene            complement(16688..17209)
                     /locus_tag="KJL99_002170"
     CDS             complement(16688..17209)
                     /locus_tag="KJL99_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000741240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBD4436891.1"
                     /translation="MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDKGITRYVTEIL
                     VKTTGTMQMLGSAPQQNAQAQPQPQQNGQPQSADTTKKGSAKTKGRGRKAAQPEPQQP
                     SEPAYDFDDDIPF"
     gene            complement(18037..18600)
                     /locus_tag="KJL99_002171"
     CDS             complement(18037..18600)
                     /locus_tag="KJL99_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBD4436892.1"
                     /translation="MHSQLRERIRLMRARLDNAAPVAEIRAESQLFVTPAPVCDRLVT
                     LAEISNRDHILEPSAGTGAILRAIRDTAPEAMCDAVEINSGLVRYLRENFNGVRVQCG
                     DFMEWQPVQYYSRIIMNPPFSHGQDIRHILRAFSLLRPGGVLVAVCLNGPRQQEKLLP
                     FSDVREVLPCGTFAYTRVPTMIIRLRA"
     gene            complement(18647..20008)
                     /locus_tag="KJL99_002172"
     CDS             complement(18647..20008)
                     /locus_tag="KJL99_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HBD4436893.1"
                     /translation="MTLAAITMTAPEAASPVQMYRATYSPDDNKLRLYAASRLDPETY
                     KKVHDAGFRWAPKQALFVAPAWTPGREDVLLSLAGEIEDEDSTLAERQEARAERFTGY
                     SGKRASESAQALDEVERLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVMLFER
                     AEYWEERARSALLHAKYKERPDVRWRRIKKIEADLRKAEKTIAQSQKYLTMWRAESLD
                     LNMAKLISSHDHISACFPLDTYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HERQIQHQQRWVNHYQNRLIYERAMLDESGGVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGAVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEEAAVASQAAKRPPVVNYPG
                     EGFREMTKAQWAALPRDCKAVRSVEEAEDHGAYRYRRTMDNNFRLVNVYITDMKITEI
                     PQK"
     gene            complement(20060..20290)
                     /locus_tag="KJL99_002173"
     CDS             complement(20060..20290)
                     /locus_tag="KJL99_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4436894.1"
                     /translation="MTVSIVSPSAAAVKPRRHPRFRREDIPTPEIDPVLKAFGRHIAR
                     SFHRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            complement(21328..21519)
                     /locus_tag="KJL99_002174"
     CDS             complement(21328..21519)
                     /locus_tag="KJL99_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4436895.1"
                     /translation="MNISTETREILRNYKAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSINR"
     gene            complement(21516..21938)
                     /locus_tag="KJL99_002175"
     CDS             complement(21516..21938)
                     /locus_tag="KJL99_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HBD4436896.1"
                     /translation="MYCTVKEIIREVLNTDVPDSECVFAVVLTRGDVRHIAQDWSLTD
                     DELETVMQRLDDAFAHGADVSIVHDVVRELMEEKRASRQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGEHMS"
     gene            complement(21985..22410)
                     /locus_tag="KJL99_002176"
     CDS             complement(21985..22410)
                     /locus_tag="KJL99_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HBD4436897.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAQKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTQLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            complement(22828..23604)
                     /locus_tag="KJL99_002177"
     CDS             complement(22828..23604)
                     /locus_tag="KJL99_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4436898.1"
                     /translation="MNENSTLNALICRHARNLLLAQGWPEETDVDQRNPKYPGWISIY
                     VLLDAPRLATLLINRHGGVLPPLLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQV
                     SFPYAGEWLTEDEIRAVLAAVRDAVRSVSCRVAEDARRIRAALTTTGQTLLTRQTRRF
                     RLVVKESDHPCWLDEDDENLPVVLDAIVNRGARFSSVEMYLVSDCIEHILSSGLACDV
                     LRIPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(23655..24089)
                     /locus_tag="KJL99_002178"
     CDS             complement(23655..24089)
                     /locus_tag="KJL99_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
                     /protein_id="HBD4436899.1"
                     /translation="MYGTCETLCRELAVKYPGDMPLMLVIWSPEEIQALADGMEISLT
                     GHEIRTVLARLEDIPEDQRIESGISSAAVMEIIRNESENRLVTVPAELLASLIQTAEQ
                     ALWKREWAARDHGLAVPECVTRRQEVVNQARTLLKNNTHEND"
     gene            complement(24103..24324)
                     /locus_tag="KJL99_002179"
     CDS             complement(24103..24324)
                     /locus_tag="KJL99_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4436900.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMEQRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELEACFKD"
     gene            complement(24325..25008)
                     /locus_tag="KJL99_002180"
     CDS             complement(24325..25008)
                     /locus_tag="KJL99_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HBD4436901.1"
                     /translation="MSRFILGDCVRVMATFPGNAVDFILTDPPYLVGFRDRSGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(25085..25378)
                     /locus_tag="KJL99_002181"
     CDS             complement(25085..25378)
                     /locus_tag="KJL99_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002430945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4436902.1"
                     /translation="MFLGFVWRVTKDVAGLLFLLRRLTRRGRNAACGQCPASGRARDG
                     RFYRSRLALVTVYASPSPFSDERPSSRFRGISSPSKRRRLRYSTVGLTRYRTR"
     gene            25525..26487
                     /gene="parM/stbA"
                     /locus_tag="KJL99_002182"
     CDS             25525..26487
                     /gene="parM/stbA"
                     /locus_tag="KJL99_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HBD4436903.1"
                     /translation="MLVFIDDGSTNIKLQWQESDGTIKQHISPNSFKREWAVSFGDKK
                     VFNYTLNGEQYSFDPISPDAVVTTNIAWQYSDVNVVAVHHALLTSGLPVSEVDIVCTL
                     PLTEYYDRNNQPNTENIERKKANFRKKITLNGGDTFTIKDVKVMPESIPAGYEVLQEL
                     DELDSLLIIDLGGTTLDISQVMGKLSGISKIYGDSSLGVSLVTSAVKDALSLARTKGS
                     SYLADDIIIHRKDNNYLKQRINDENKISIVTEAMNEALRKLEQRVLNTLNEFSGYTHV
                     MVIGGGAELICDAVKKHTQIRDERFFKTNNSQYDLVNGMYLIGN"
     gene            26490..26840
                     /locus_tag="KJL99_002183"
     CDS             26490..26840
                     /locus_tag="KJL99_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001630992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HBD4436904.1"
                     /translation="MDKRRTIAFKLNPDVNQTDKIVCDTLDSIPQGERSRLNRAALTA
                     GLALYRQDPRTPFLLCELLTKETTFSDIVNILRSLFPKEMADFNSSIVTQSSSQQEQK
                     SDEETKKNAMKLIN"
     gene            complement(26963..27244)
                     /locus_tag="KJL99_002184"
     CDS             complement(26963..27244)
                     /locus_tag="KJL99_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4436905.1"
                     /translation="MNYDEITKITAERISDYMTEAVNTDSKSVAEMFHNAAWGVLSLW
                     FELVTKIDLDIHKKNRYASYDFRRKIEMQHEEFQKMTEREQVPLLKLPE"
     gene            complement(27372..27606)
                     /locus_tag="KJL99_002185"
                     /pseudo
     CDS             complement(27372..27606)
                     /locus_tag="KJL99_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421253.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
BASE COUNT         6655 a         7587 c         6688 g         6694 t
ORIGIN      
        1 atgaacgggc aggaaatgga catttccggg ttcagcagat tgctgtagtt gtcggccatg
       61 ttccacagga aggcgatttc cgggttacgg gccagatgga aattcaggat gcgggccgtg
      121 ctgttcctgc cgttctcacg caggcccagc gtcaggtaat cagcccggac attcaggtca
      181 aaactgtcct ccacacactg ataattcaga tcagaatacg ggtccagctg acgtcttttc
      241 gggtacagag agtccggatt atggttaatc atctccccga caatctcgat gaaagcctgt
      301 gcatccactg tctgtgtggt gatttttgcg ccgtgaaaag aggcacggat gattttcacc
      361 agattttcca tttccagaat gtcagcccgg ctttttttct tcgagggaga acagtacgag
      421 ataaacaccc ggtaatgacg cagggtcagg ggcagattca tcccctccgg cagcgggaac
      481 tgtgtcgctg ccgctttcat ataataggca cgggtaatgg cgttaaaccg ttcagcctgt
      541 tcacctgacc aggagaactc acgcagcccg tattcaatcc tgtcaccaac cagctgactg
      601 gacatcagat ggatacacaa cggaataccg cgcggcagtt tggtgcgcag catgtgatcg
      661 agtgcctcca caatggactt attcgcccca ttgatgggaa tggcttccag cataaagccc
      721 atcgtggtgt cattcatgaa cagaccggat tcctggttgt aatcacggta cggcagcaga
      781 cggctgaact gcgggaagct catttcgccc agaacttcat tggcctttgc ggattcgtca
      841 ggcagtttca gtgctgtgac gagggagtta accgcctgag tgacggcctc aagtgggtta
      901 ttactcatat gagtattcct tagtgcgttt aaaaaaggaa aatatcagtc tgtggccgtg
      961 gatatatcac gcagtgccat tcctgaaacc acagcacaaa taagagatgg aggcttttaa
     1021 caggaaacca ggattaacgt tcctttactg aatgagcatc atttaatgcc tgaatttgtt
     1081 tttcgcgaat cttattgcgc tcattttctc ttacccgttt cacctctgcc atttcgtttc
     1141 tttttctgca ttctgatgcg attttatttg ccttaccaag tgttaaaggt tcagactgat
     1201 aattaagtgt ctcctgaaat gcttctgctg cacagggagt ttcctgtaaa atctctgtca
     1261 gttgttctgg tgttgcaggt tgttgcgcaa ggtaaacgat gtaaaaagga atacatacaa
     1321 tcatagccat aacgccaaca aatataaaag ccagaacaga ttttaaatat atcgacttca
     1381 tttcattttc cttcgaagca gtaagcactc attgcccacc accatttagt gtttccatca
     1441 cgctgttgaa aggttcattt gttttaactt caatacactc ccctgtattt ccaaagcccc
     1501 tgccgccatg tgcttcgatt gtccgaagaa cctgcacgca tacgtccgta tttggcatgg
     1561 gtgtaatatt ccagcttccg ccaccgttaa tattttttat cagcacaaga aaaataaagg
     1621 tatgcattct ttattatctc cgcttgtata ttaatcactg aatatccctt cagttaactt
     1681 atgaggtcat aaaacactgc agattttcat tcagatttac tctcttcagc aaactttact
     1741 ctcttcagca aacttccgga tggtatcctg ttcacgctga cgggtggcag actcctgcat
     1801 tgcatccact ttctcctgcc agagacgccg gatatccttt tcatcccgac cagtcagttt
     1861 tttaccggac agcagacggt taagcagttc ctgtctcgca tccggtacgt cttcagtcag
     1921 acgcataagc tgaagcaggc tctgacggct gagaaccatg agtgattccg gcaggagggt
     1981 gaagaagaaa ataaaaaagc ataccgcacc acagcaggca aggaccgcaa acaggatttt
     2041 cagatatata ccatcaacgc cgaacgcagc aataatacag ggaatccatg caatacacgc
     2101 acaatacgcc aggatgttat ctattcggtt atgtctgcga tatatctgtt gatgttcatc
     2161 cagaatagaa tctatttttt cagcttctga tttatgcata gtgtttcctc tgcgtttcct
     2221 gccagagacg ccggatatcc tgttcatccc gaccagtcag ttttttaccg gacaacaaac
     2281 ggttaagcag ttcctgtctc acatctggta cgtcttcagt cagacgcata agccggagta
     2341 attcctgacg actgagtacc atgcgtgatt cctccggtac gctgtaataa ttaaaaatac
     2401 gactgaatac cccacaacag caggcaacgg acaggcacag ggatttcaga tacaggccag
     2461 aaacacagaa tgtggcaaca ccacaaagaa tagctataac taaaaatccc ttacccagtg
     2521 ccatacacaa agaaccctgt tttctggata tccgctcatg ctcatccaga ataaaatcta
     2581 ttttttcatt ttctgattta tgcatagtgt ttcctctgcc tttcctgata cgcctgacat
     2641 tcaacgcaca acgtcacacc gggaaatatt ttccgccggg cttccggaat aggatttccg
     2701 catgcttcac agagataaac aggaatacca tgaacattta tttcctggcg tatccggttt
     2761 attcctgtca tggtcagttg ttctgtcact gaatatgctt catcggcttc atcactcacg
     2821 gaatattcct cccgacatgt tctgacacgc taccgcccat cccttttcac gcacagggca
     2881 ctcgttttac aggcggattt tgaatacaga aagcccgccg gcattgccgg ctgatataca
     2941 acttcagggc cagttaatta atacgtggtt ttccccacgc agacggttta ataacaaaaa
     3001 acacgctgga tggctgatga taaacatcct ggttatcaat ataaggcgct atccataatg
     3061 ctgccgtctg ctcacccgtt cttagcggac gaggaggtgt taccggcgta actgaactga
     3121 ccggaacaac cgttttcact tcccgggcag cggtaaacag cgggcgtggt gccagcagtt
     3181 tctgctccgg atgtgctgtc accgcaggtc tgctgccgga cggagtggtc gcggtgacgg
     3241 tctgcaccgg cattgtccgg aagttccctt cagccaggcg cggcagtgat gccgcaaccg
     3301 gctttgcttc tgaggactgc tccagttttt tggccttctc attggcctgc tccatcgtca
     3361 tacaggtatc ggatgtggtg gcgttacact caaattccgt atttgttcct gcgcagccgg
     3421 aaagtaataa ggttcccagc agaggaataa ataaagaaat ctgtttcatt gcccttctcc
     3481 ttttttatta ctccccacaa cagcagaaat taactctgct gtcagatttg ttttccctgc
     3541 acagggatgt ccggagacca ctgtatttcc cggagaagaa aataagctca tcgacattcc
     3601 cttcttcatc acaccgacag cagccatatt aaatttgtgc ggcaatgatt cagaactgaa
     3661 caccttcgga gcaggaagaa ctatttttgt ggtgcagtta ctgagaacag catgcataaa
     3721 atcatctgat acgtttttat ccggcacaac gcgggctgta ataacattaa tattacgcat
     3781 gttcattttt atctccgttg cttctgtttg ttcggtcatc agggaaataa acatggcatt
     3841 tcctgatttt tcccggcagg gaagcgtctt ccgtataaac gtccttcaga tattcatctg
     3901 cggttaccac ccggatacat ttcatttttc cgggcagcac cggatatttc ccggtgatcg
     3961 ctgccagcga gacatacatc agtgccagtg ttgcccataa tgcggtgacc agttgcatca
     4021 gtgaccggtc agtctccctg acataatctg cagcatcagc cgcgcggtaa tggcagatat
     4081 cctgtatttc tgatctgcct gaagaatcag aggaagaaaa ccagcattca ctgaccggga
     4141 actgtccggc aggtgctgtt tgtcttacga agttaatctc cttaaccagt aatttccccg
     4201 gcgtggtgtt gtccttccat aaaacaaaca gagccatcag ggccattgtg gccagtggca
     4261 gaatgacggt gtacttcaga caccagaaca gtcctcgcag aacccgggcg acaacatacc
     4321 gggcggcatg gcacgcctga cgggacgaca tattatttgc catcgttgcc tcctccctgg
     4381 ctgccggaca taaactgctg cggattcagg ttgcccagct gcttcagcat ctggtcagta
     4441 ttaaagccgt taagatgact ttcagctgat ggcggaacag gaaccggact ttccggatta
     4501 tcttcttccg ctctgttctg cgtgcgctcc agcgccatct cttccacggt tttcagctgg
     4561 aagccgtcct ggaacaccac ggtcacttcg ttgcccgcac caatcggtat caccgggtga
     4621 tactgttcgg cacgtttgat gtagtagtca ctgagcgtct gtgcggcttt cgatgtgccg
     4681 ccaccgatac ccattttcag gacatcacca gccccgtaag cggctgtggc acccagcccg
     4741 acagccggct gggaggcgcg ctccattccc tgaccgatac cgtcaacaaa tcccgcgccc
     4801 catgcccagc cgaggatttt gccgttacgc atcaccactt cgcccttgat accgttttta
     4861 ccccggaagc tgacatgccc cttaatcggc atatcaatag ttttgccgtc cttcaggcaa
     4921 ctgatattgc gggtgcgtac aatggcacgc tcactggaca catcccccca ggcttccagc
     4981 cccacaaaac atcccgttgc gtcatacgtc ttgctgttcg gcatttccac caggccggtg
     5041 atacgcaact gcatcggcac cgtggattca ttaccggtga ctgaggcatt ggcatccgcc
     5101 ccttcaatca gcatggcttt cgcaaaactt cctgacggaa tgtacggcag cgatggtccc
     5161 tgttttccct cattacgggt aaacacctta cgctgtatcc ggttaggcac cggcacagac
     5221 tggtacgtca cctgtggtgg tggcgtgaca ccattcccgg gataaaacgc cgtcggcggt
     5281 ggaacagcaa cgctgccctg cggcgggaag gatacggggg tgtttcctgg ctgtggttcg
     5341 ccttccggcc cgggcggtga ggcaggcatc tgaggtacag gctcaccggt cgccgtgacg
     5401 ggattagcac ccagggcttt tacctgctct gccagggcgg cattgtcctg tcccagcttt
     5461 tcaatacgac gctggtcatc accgcgctgt ttgttcagga catccagttc acgacggatt
     5521 tcctcatact gcttctgcat ctgcgctgcc gtcacctgca tctctgtggt ggcatgctga
     5581 cgcaccttgt catcaaaggt cgtatccacc acacctgtca tatccggcac aggctcctgt
     5641 tcagccaccg cctcaccgtt accggacatg tccacatcag acagatacag taccccacca
     5701 atcgcggagg ctgcaccgac aaccacaatc cccagccaca ggtactgctt gcgtttcaca
     5761 atggtattga tactggccat cagttgccct ccccgtcacg gatgacatac acatccatgc
     5821 gcgcacctgc cagtaactgg ctggcaggct ggctgaacat cacggcacgg gttcccggct
     5881 gccagaagtc actttcccgg atattcaggg cagacagcgt tttattttcc acggcaaagc
     5941 ggaccatctt caggtgattc cccgtccata cggcatctgc cactgaagac agccccgccg
     6001 gggcctgcag ggtttccttt gtcacaggaa cctgatacca gcctgcaggt aattttccgc
     6061 cacggacagc ctggctgatg gtcacaagca gggattcgta aggcgtggac gtttcccacg
     6121 ctccggcttc ttctccggtt ccgcgcaggt cactgaccag ctgaatggta cgccccgcgc
     6181 cttcacgggg aacagcctga atggaaaggt tcagaccacg ctctgtttcc agaatgaacg
     6241 taaagggctt tttgttgacg gtggccacca ccacaccgcc ggaggcggtc tgctcattat
     6301 tggtcagggc accatccaga ctgttcacgg cgataatccg gtcaccggga acggtaaaca
     6361 gattcgggct ggtattgctg atggcaatac tggcctgacc accgttcacc attggcacca
     6421 cagtgggggc cagcgtaccg tttgcggcca tcacgctgca ggaaaaaaac aggccgccga
     6481 atattattgc cgtattattt tttctcatcg tctgtttccc cgaaattatc cagccaggtc
     6541 accccgtttt cccgcttcag aataaggatg taatgtttga tatcagtaaa aggttcggag
     6601 ttaccaatcc atgttttccg aattccacgg atttccacac gaccatactg aggccagaca
     6661 cggacactgg tctggaaaaa agcagaattc acgttatctt ttttaatgag tttcgcttct
     6721 tcagccagaa taactttcat ctggttctgt gcacccgggc ggatatattg cagaagcgcc
     6781 tgatgtgaag catcgacgat ttccggtgag acattcagac ggagggcaat aaatgacagc
     6841 gccatctgct gcaaataaga ggcatcggca ctgttctgtg acacggcaaa gggcacatta
     6901 aatcccattg gggtcacggc agtgcgttgc tcattctgaa gacggtagtt attcaccccc
     6961 tgaatgaggt taacagagag gctgagaaca ataagcactg acataaatat aaaggcgatg
     7021 gccattacac gactggtact taaacgggca ccgtgttcca tataacaatc ctggtatcag
     7081 ttctatttaa tccactgccg gaaacacgaa tcgggaacat tatgaaaaat accgcgcagc
     7141 agggctgttg gcatatacca gtaaatcagg tcacgtaacc aggaactgcc ccgccctttt
     7201 ttcagttttt taatcccgaa ataaaccaga accgctgcac caataccgaa cagatatttc
     7261 gatgttgtga taccccagcc aacacagatt gctgcgggga tcagttcatc cagtggcagg
     7321 ccaaaccagc ggctctggtt ggtcagtgtt tccgggaaac gatatttctt aagtttattc
     7381 tcgtctcccg acatcgtttt atttcctgtc agaggccaac gacgctcata cccacagcaa
     7441 taaatacaga gatgatggca aagccgacca ggaacttgac gtttttggtc atcatgtaca
     7501 tgaccgcacc gaccagcacc tcagccagaa caacccattt aacgatactg gagtccttac
     7561 cgaaggtcgc cttaaccgta tcgttacctt tagccatcag gtcgccttta ccagcagcca
     7621 tagctaactc agggaacaac atcatcagaa cagcaaccgg gatcactgca cgaaccaggc
     7681 gaagcatatt cagacgagtg aatttagaaa aaaacgattt attttcaacg ggcgcagaag
     7741 caccctgaac acttaaaaca gtattcatat gattcctctg attaatattg acagatgaaa
     7801 actaaagttc tttaaatgtt gactcgttct cttcgatcac ctccctgaat atttcttcat
     7861 tataaaaatc tggataacgt tgcaaatgat cctgcaacct caccaatgct tcgtttgttt
     7921 tcgttcttcc actacgctct ctggctccaa gtaataattt attcgtttcc tcatcaaggg
     7981 taagtaaaac ttttctactt ggcgcagatc ttaaagctcc tttaatcacc ctccctccca
     8041 taaataaaag agatttagtc aaaatattca cataagacgc acacaaaaat aaacaacaaa
     8101 atagtaaatt acgttaacaa aaaccggcac ttttagtgcc gggaatgctt acatttcttt
     8161 acagtgaagg ctgttaatat tactagcgaa gtattgatat agataaaata acacatcaga
     8221 gattatagca tatcgcaacc actcatccct gtctgagaaa ccatagtgtt ttttaaaatt
     8281 cataaatatt tttttagatt ggtcaacaga gatatccatt ctctgagaag aatactcatg
     8341 actataaccc aaaagatacg gtattatctt attaaagcga tatttgtcag gttcacaact
     8401 aaactccttg ccctttataa aaaagccact aactacgtat ctttcaaaga atctccagac
     8461 aaacaaattc aagttattca cttccgtact ttcaaccacc acatcccatg atacatcatt
     8521 caatattaca ccattaataa tactcactcc attcgaggac gagaaagcat caacttcagc
     8581 agtatgaaat aaaatttgcg tatcaatatc aataccagaa aaccatattt cagaatcact
     8641 attctgacac tttattaagt gtaaaaaaac agagtttgag tctatgaatt ttcctgactc
     8701 tgtgcggaca cagactggat attttgcgga taaaataatt tccggcaggc ctgattgacg
     8761 tatgagtgct ctttctctag tatccatcgc acacatagga acctcctcaa aggattctat
     8821 ggacagtcga tgcagggagg gacaagctcc ctgcatcggc gattttcaag ttcaaatttt
     8881 aacgtggcat taaccacgta attaatatac taattattct gattattgtc atcagtttga
     8941 accattaaaa agtctgatat ttgaacaaag tcaaaattta tttcccttta ttcatcatca
     9001 ttttttggaa agaacctttc catctcagcc gacaccttct ctctgatatc ttcaaccata
     9061 ttggcatatt caaactttgg atttcctgag acatgaggac tgagagactc aatacccaaa
     9121 attttcgcta ctgatgactg tgtttttaca acgcattcaa gaagtagttt attaaactcg
     9181 gcctgattaa atgcagactc tttgcgctcc atctgagcct catatacacg aagacccagt
     9241 tcaagaagca ttgaagccgt tgctgaaaaa ctgacatctt tttctctcgc cccctcctgt
     9301 cgacgttttt caataatcgc gtttattttt tcataagcat cattgctgat atacagattc
     9361 accttagcca ttagatatta ccttttttaa acataaaaca aagttaattt cgaaacacta
     9421 gaattcatag tgcaaacagg gacgcaccgc tagcagcgcc cctgacggta tcctataaaa
     9481 aaacacaccg cgccgctagc agcaccccta atataaaata atgtttttta taaaaatagt
     9541 cagtaccacc cctacaaagc ggtgtcggcg cgttgctgta gctgcgttaa cgacgctgct
     9601 ttaaaaaaat cagatttaaa caatataaat ccacaaatac aactcaatga tattaaagat
     9661 aaatcagcaa aaacttgttt ttgcgtagtg tgtggtgctt ttggtggtga gaaccaccaa
     9721 cctgttgagc ctttttgtgg agtgggttaa attatttacg gataaagtca ccagaggtgg
     9781 aaaaatgaaa aaatggatgt tagcaatctg cctgatgttt ataaatggga tctgcgaagc
     9841 cgccgattgc tttgatcttg caggtcggga ttacaaaata gacccggatt tactgagagc
     9901 gatatcatgg aaagaatccc gttaccgggt taatgccatc ggtattaatc cggtaacggg
     9961 atatggcagc ggactgatgc aggtagattc ccagcatttt aacgaactgg cccgctatgg
    10021 aattaagccg gaacatctga caacagatcc ctgtatgaac atttataccg gtgcttatta
    10081 tctggcaata gcctttaaaa aatggggcgt ctcctgggag gccgttggtg catacaatgc
    10141 cggattcagg aagaccgaac gccagaacca gagacgtctt gcctacgcat cagaggttta
    10201 ccggatttat acctggataa agagcagtaa aggcatccgg gttccgacca cgaagaaatc
    10261 actttcccaa attaacagtg tgcagaaaaa ttaatattgt atacattgtc aaataccggt
    10321 tatgccgcga cagcggcatg gaaggccaca cacaaaaata tcatatgagt gataatgctg
    10381 actggccgaa gccccgcaaa gcgggatccc atcttatata tatccggtaa ctgcgcagcc
    10441 tcctgccaga ccatttttcg cgggcacaat atttacagac taccgaaaag ccagttgatg
    10501 aaattgaggc atatcagacg acacagcaat gccgcaggat gataatataa gtaaagcccc
    10561 gaaaaatttt tcggggcttt acttatatta tcatgacttc cactccctga actgttcggc
    10621 aatcatccat aatgctttgt tgattcggat atcaccgtca atgcctgtaa tggctcttgt
    10681 tctggtattt ttaccggagg cacttcgccc cgataatcca ccttttatca tattctcctg
    10741 aacccgctgc caggttgtcc agagatcgtt ctgtttatcc tcccaacggc ggggagtaat
    10801 tatttgttct ggcgtcactg gcgttttatt ttcatcttcg tacctggcca tcaatgcagc
    10861 tctgccaaat aaatgttgtt cgtcactgtt aagatgtatc tctttcattg tctccatatt
    10921 ttcagtgact ttatcaaaga caccgagcac ttcatacgct ccctcaataa cctgcccgac
    10981 aatatcacct ttatgtggaa cgcggatttc gccaaaatta ttcccacaca ccaggccatt
    11041 tgtgcagaca aaacgaaaaa ttcctgggat catctgataa ctggatgaac catcatgtga
    11101 attaagcagg ataatttcag gaacttcttt cccgttaata tgcccttccc tgcgaagacg
    11161 taacatatgt ttactgtatt cgcggcgtcc caaatcacga acccgactct gacaggcaaa
    11221 gaatggctgg aaaccttcat cacgtaactt attgatgata ttgatggttg ggatatatgt
    11281 ataacgctca ctccgggatt catgtttatc accagaaaag acagatggaa caaactgcat
    11341 taattcgtca tccgttaacg ggcgctcacg gcgaatagaa ttatatcgac caaaacggga
    11401 agctaatcgc ataacagtaa ctcctttatg aaggcataaa attaataaaa gttttttcgc
    11461 ttaatatcat cccccctcgg ttatcgttaa caaaaagaca acaattccga ggggaaggac
    11521 catcaaatca ggtcagttcg ggactgacca acctgccgag tgaaaagcag taacagcgcg
    11581 atcaactcat cagcgttttt tctgacaaga ggagtatgat tctccaggct atccagtaag
    11641 tcatgcagct cattattgac ctggtggagc accttaaaga gtgcaccaga atcccggcag
    11701 ctctgatatg caagatttgc ctgaacagca cgattctgca tgatcccgat ttgtgcgatt
    11761 gcgtctttct gctgatcgcc tgttggctga taagtggtgt taaaagaaaa tgcgctcata
    11821 attcctcctg tatctgatgt tttcagcccc catcatctgt tgagtttcgg gagccgcggt
    11881 tttgacgttt tgcggggacc tgccttttgt tccctccggt attcaccatc acctgtttga
    11941 gaaccttcgc cgcaaggggc atcactgcca cggcctgacg cacctgtcct gggcagaacg
    12001 caaaattttt taccgggttt tgggtaaaaa attttgtggg ctgttcattt accctggaca
    12061 gtcagtgcgg caggttgtgg ctgtctatgc ccccggcgac aggggcttaa acaggtgaat
    12121 ggcaccggac agaacaacgg gcagggaccc gccgcaacga cgtgaagcgc ggttcgcgaa
    12181 acggcgttgc tccttacatc ccggcgtctt ttgccgggat gccgggagcg caggccacgg
    12241 cgcgacaagg gtggcgcgac cgttccggtc gctgcaaacc cgggtttttg cgtcagggat
    12301 ggaagcccgc cagggcggag acagcttcgc tggctccgtt cacgacagcc cgccccgcca
    12361 gggggacgcc cggaaaaggc ataagcagga gaacaacaaa acagacaata atcagggcag
    12421 cagtaatcag agtcagagca gtaatcagag tcagagcagg aggaaaaaga gaataatcaa
    12481 agggaaggtg atgtgatttc tggtaagtca taaacagaga aggcggggga aaagaacggg
    12541 cgccgcaggc gcacgttcat ccccgttccc ggagggaaac ggccttcctg gcagtctggt
    12601 tgttcatgcc cgtacaatgc cagcaaggag aaaggggcca ccggcgaacc agcagcccct
    12661 ttataaaggc gcttcagtgc agtcagacca gcatcagccc tgaaaaggcg ggcctgcgcc
    12721 cgcctccagg ttgctactta ccggattcgt aagccatgaa agccgccacc tccctgtgtc
    12781 cgtctctgta acgaatctcg cacagcgatt ttcgtgtcag ataagtgaat atcaacagtg
    12841 tgagacacac gatcaacaca caccagacaa gggaacttcg tggtagtttc atggccttct
    12901 tctccttgcg caaagcgcgg taagaggcta tcctgatgtg gactagacat agggatgcct
    12961 cgtggtggtt aatgaaaatt aacttactac ggggctatct tctttctgcc acacaacacg
    13021 gtaacaaacc accttcacgt catgaggcag aaagcctcaa gcgccgggca catcatagcc
    13081 catatccctg cacgctgacc acactcactt ttcctgaaaa taacccgctc attcaggctg
    13141 tttacgggta atctgtgtga ttgttgccgc atcacgccgc ctcccggagt ttgttcggca
    13201 ccatccaccg ctccatctca cggacagcgt gaccggcctc tcttgctgca agactggctt
    13261 ccgcaataac ctgccagaac ggcatatcct gccactgcca gcgttccatt ccggccagtg
    13321 acggaaaacg ctcaccccac tgccagaaca gggtttccag gtcatgctct gccacccggt
    13381 cactccagcg tgcataccag cttccgactg tttccggcga atggaatgcc agttcgattt
    13441 cagcctgagc gaccagcgcc tggtacgcac gggctttctg ctctgcctcc ctcgcctgct
    13501 ggcggctgta cttctgcatg accagcgcgg atttatggcg actgcgttcc ctgacacgaa
    13561 accgcaccga cgggaacaag cgttctgctg catcgcctgg aagaggaagg aggcagacct
    13621 cgccctgtgt ttcaatcagc acatcgatgg catacagcca ctgaagacgg ctgccgtgca
    13681 gttcttctgc ggtcagggac ggcatgaaaa atcgcaggtc agacagcgat atcctgcggc
    13741 ttccgttcag acagcggaaa aacgcccccg catacggata ttccgccagc gtgtttccct
    13801 ggtggcggcg tttttctgtc tccgccaccg cccgccatgc ggcctgttgc tctgcgctga
    13861 ggggaaccag tgcctgtgaa cgtgcgctca tgcctgacct ccttccgcca tcaggatatg
    13921 gatgatgtca ctgacgctgt acccctgcgc gtccagtgac gccgccagtg ccagtgaatg
    13981 gctgacggct tccggtgcaa accgccgctg tacctgaacc accgcaaacg gctcaccacc
    14041 ggcattcacc agaaccagca cccagacctg agagacatcg cccggtgaac ataacgccag
    14101 gtgaaaacgt tcgtgggcag gcgtgaaacg gacctggacg gggaaaaatc cgccaaactc
    14161 gctgccgtac tcgccgttca tcgtccagtt gtccggcgca tccagttccc gcatcacggc
    14221 gtgtgtcagc tcacgacgtt catcacttcc ggcagcccgg atatcttcat attcctgtgc
    14281 gctcatggtc tgtaatacat tcagggtcag ttcagttttc atcacggtat ctcccttgct
    14341 gccgccccgt ctccggcggg gcggtgtggt tgttcatcag gcggcatcag ccaggttatc
    14401 ggtgcgttca gtggtatctg tctgtggatg tggtgcacac atccagccag gcacccagcg
    14461 gttgtctttc atatggtgct ctgcatgttc agccgcatca cctttcttca tcttctccgc
    14521 gtcccgtgcg gcaccggaca gccctgcctc attcagggct gcgataatct gcggcttttt
    14581 caggtgtccg aagaagtttg cttttgtcgg ctgccaccag tcgcgcatgt gaaagccgat
    14641 ggcgctttcc agcgtgtcaa gcggactgcg tgacgtgtga ccacactcac gggtctggac
    14701 accgttaagg ctgcatgcgg tgcagaaact gagcagggat aacagcacct cctgactgag
    14761 tgacaggaat gtcgtcatgt cccgtgacca tccctccggt aacagggcag caagacgggc
    14821 tttttctgcc atcagcgcca tgaatgcggc accttccttc cccgatggcg catcgctggt
    14881 cagcgaataa tgtttacatt ccaggctgat ttgtgctggt ttactgtacg ctccgctgcc
    14941 aaacacattc aggcagagcg tccatgccag cagtgtcagg gatttgtccg gttgctgcat
    15001 cagtgctgcc tggactgcca gcgtgcgttc ggaagacatt ttcgtcagca gtggcagact
    15061 gatttcctcc actgacgctt tctcctgcac ctgttccgta cggtcagcgt cgtcagccac
    15121 gtcatcctca ctgcgcaact gcacaccacg ctggacacac acgttgccat aacgccagga
    15181 caccaccact ccacctccgg cacgcatctc cggcgtccac gccctgacct tcgccatgca
    15241 gcgcatcagc ttcatttctg cttccagcag gtcagattcc tcacactggt tttccagcgc
    15301 gtcgtaacgc gtcatcagtt cgttcagacg ttcttcttcc gcctccgtca gcaccgcttc
    15361 cggctccggc agacagcggt atgttccggc atcctcacgg cactcaccga caggctccat
    15421 gcgtccggcg caccattccc agccttcggc ttcccgaagg aactctgcga cagcctggag
    15481 tttttccagc agggcggcat cgagcgccac gcagtccaca tagccacctt cgtcgtcgct
    15541 gaacaaatcg gtgcgcagtt cgtctggcga gaaggcatca gcccccacga agcggaattt
    15601 actgttcccc gccaccgcca cttcactttc ggtcaccaga cgacgaatgg tctgtacttc
    15661 cggtttaccg ccccatcccg actgacaggc ggcttcaaac acctgcacct gacgcgcggt
    15721 gtcgttctcc agcgccagcg cctgacagtg ttcggtggtg atgcggtctt ctgccagcgc
    15781 atcgaggatg acaggcgcaa ggtcagccag tttcagcatt cgctgaacgt ggcggggtga
    15841 atagcccagc aaatcaccga tttgtgcagg tgttttgcct tcctgcgcca ttgcgcggaa
    15901 tccggcaatc tgttcggcag ggtgcatatc ccgacgctga ccgttttcag tcattgatgc
    15961 agccgtcgcc agctcctgcg gaataacctt cacgcgtaca ggccagtcag ccggaaggat
    16021 gccacgctct gccagcatgt tgagtgctgc cagtcggcga ccacctgcgg cgacaccgtg
    16081 gcggtcacca ggcagggcat gaacaaccag attctgcaac aggccaacgc ccttaatgga
    16141 ctccgccagt tcgctgacgg actctgcgga atacggcacc gtgcgcacat tcagcggtga
    16201 tttaatcagc gaggccagcg acacgctcac ttcctccgtc tgctccagca gggcagacag
    16261 gactgtttcc tgcgtttttg caggtttacg gctggattta cgctccgttt ttgtcttaga
    16321 ctctgtaact gacatatatt tctcctttga ccgggatggt tgtatgtctg ccggacgcgt
    16381 tcccgtccgg cacctctctt acttaagaat gccgaaattc cggatgacat ggttcatcca
    16441 gtacccggca ccgtcctcaa aattccaggt acgccagacc aggccactgt tattgcggac
    16501 aaccaggcga aaatgcgttc cctgatcatc ctcgataaag acattccggt actgtgcggc
    16561 aacggcttca gcctgttcgc gggtaaagga gccgtcaggc agcccctgaa atattctgaa
    16621 atattcgctc atcctcatat ctccggtgat gccccgcact ggacggggcg gttgtcactg
    16681 tctttcgtta gaacggaatg tcgtcatcga agtcatacgc gggttcagac ggctgctgag
    16741 gctccggctg cgcggcctta cgtccacggc cttttgtttt cgcgctccct tttttcgtcg
    16801 tgtcagcact ctgtggctga ccattctgct gaggctgcgg ttgcgcctga gcgttctgct
    16861 gtggtgcact ccccagcatc tgcatggtgc ccgtggtttt cacaaggatt tcagtgacgt
    16921 aacgggtaat acctttatct tcccagctac gggttcggag ttgcccctcg atgtagacct
    16981 gcgcgccctt gcgcagatat tcacctgcca cttccgcgag cttgccgaac agcaccacgc
    17041 gatgccattc tgtctgctcc cgcatctccc ccgtctgttt gtcacgccag ctttctgacg
    17101 tggccacctg caggtttgcc actgcgcccc cgttggggat gtaacggact tccggatctt
    17161 ttcccagacg accgacaaga atgaccttgt taatgccacg aactgccatg atgtgtctcc
    17221 ttctgttgat gttttcagcc cccatcatct gttgagtttc gggagccgcg gttttgacgt
    17281 tttgcgggga cctgctcttt gttccctccg gtattcacca tcacctgttt gagaaccttc
    17341 gccgcatggg gcatcactgc cacggcctga cgcacctgtc ctgggcagaa cgcaaaattt
    17401 tttaccgggt tttgggtaaa aaattttgtg ggctgttcat ttaccctgga caggtgcggc
    17461 aggttgtggc tgtctatgcc cccggcgaca ggggcttaaa caggtgaatg gcaccggaca
    17521 gaacaacggg cagggacccg ccgcaacgac gtgaagcgcg gtttgcgaaa cggcgttgct
    17581 ccttacatcc cggcgtcttt tgccgggatg ccgggagcgc aggccacggc gcgacaaggg
    17641 tggcgcgacc gttccggtcg ctgcaaaccc gggtttttgc gtcagggatg gaagcccgcc
    17701 agggcggaga cagctccgct ggctccgttc acgacagccc gccccgccag ggggacgccc
    17761 ggaaaaggca taagcaggag aacaacaaaa cagacagtaa tcagagcagc agaaaaaaga
    17821 agaacagtag cagtagcagt agcagtagca gtagcaggaa caacaacatc atcagtaaca
    17881 ggcaaatgac aggtctgaaa agacagaagc ccacagcaga acgtccgcgt aatgaaaggg
    17941 gcggcgcgta agcgcggccc ctttcagcgc caccggcgcg cggtcttttc ccggcgcggg
    18001 cagacgggca tttatcctcc cgcctgcccg gattcatcag gcacgcagac gaataatcat
    18061 cgtcggaaca cgggtataag caaatgtgcc gcacggcagc acctcgcgga catcagaaaa
    18121 cggtaacagc ttctcctgct ggcgcggccc gttcagacag acggcaacca gaacgccccc
    18181 cggacgcaac agggaaaaag cccgcaggat atgccggata tcctgcccgt gactgaacgg
    18241 cggattcata atgatccggc tgtaatactg caccggctgc cattccatga agtcgccaca
    18301 ctgtaccctg acaccgttaa agttttcccg cagataccgg accagcccgc tgttaatctc
    18361 caccgcatca cacatggcct ccggcgcggt atcccgaatc gcccgcagta tggccccggt
    18421 gccggcagag ggttccagaa tgtgatcacg gttgctgatt tccgccagcg tcaccaggcg
    18481 atcacagacc ggggcaggag tcacaaaaag ctgagattca gcccggattt cagcaaccgg
    18541 cgcggcgtta tccagccttg cgcgcatcag ccggatacgt tctcttaact gactgtgcat
    18601 gtcattatct ccgtcatccc ccggggattt cccgggggga tataccttat ttctgcggga
    18661 tttccgtgat tttcatgtcg gtgatataca cattcaccag acggaaatta ttgtccattg
    18721 tgcggcggta gcggtatgcc ccgtggtctt ctgcttcttc cacactgcgc acggccttac
    18781 agtcccgggg cagggcggcc cactgcgcct ttgtcatttc ccggaaacct tcccccggat
    18841 agttgactac cggcggacgc ttcgcggcct gactggcgac ggcagcctct tctgccgatg
    18901 gtgctttgta gtccgtgatg cgatcgggcg tcactttcat cgtgccgctg tacccgagaa
    18961 aactgtaatt tggcgttgtg actgaactca ccgccccgtt gcttttgttc acgcggatga
    19021 tagtcagcca ctcgccccgg ctgaaaacct gtccgcccgg ctcaaaatcc tgtgtccggg
    19081 taaccacgcc gccgctttcg tccagcatgg cacgctcata gatcaggcgg ttctgatagt
    19141 ggttaaccca gcgttgctga tgctgaatct gccgttcatg acagcggatc gcaatttcgc
    19201 gggcctgctc cgtggtgatg atgtcatcat ccagggccga ccataacgac cggctccctt
    19261 catactggct tttttctgcc gggcgcggat acgtatccag cgggaaacag gcgctgatat
    19321 ggtcatgact gctgatgagt tttgccatat tcagatccag cgattcagcc cgccacatcg
    19381 tcagatattt ctgcgactgt gcgatggtct tttcagcctt gcgcaaatcc gcttcgattt
    19441 ttttgatacg acgccagcga acgtccgggc gttctttata cttcgcgtga agcagtgccg
    19501 accgcgcccg ctcctcccag tattccgcac gttcaaagag catcacggca cgtttcatgc
    19561 cgttttcaat acgctgcgca tcacgacggg cgcggcgttc gctgtgatgc cccacaagaa
    19621 tgggctgacc gggcgggatc atcgcggcca gtctttccac ttcatcaagt gcctgtgcgg
    19681 attcactggc ccgctttccg ctgtatccgg taaaccgctc cgcccgtgct tcctgacgtt
    19741 cagcgagcgt gctgtcttca tcctcaatct ctccggcaag tgagaggagc acgtcttccc
    19801 ggcccggtgt ccaggccggc gcgacaaaca gcgcctgttt gggtgcccag cgaaaaccgg
    19861 catcatgcac ttttttatac gtctccgggt caagacgtga cgcggcatac aggcgcagtt
    19921 tgttgtcatc cggtgagtag gtcgcgcggt acatctgcac agggctggcg gcttccgggg
    19981 cggtcatggt gattgctgct aatgtcataa aatgttctcc ttccgtctgt tgccgcccct
    20041 cgcggggcgg ctcacctcat cagttaaaag cgcgtttcag ttcgagtgtg cggagcacct
    20101 gcccgaaagc cgtgtttttc atggcgggga tgtgtacacc acgcccccgg tgaaaactcc
    20161 gggctatgtg tcgcccgaag gctttcaaca cggggtcaat ttccggcgtg gggatgtctt
    20221 cgcgtctgaa acggggatga cggcggggct ttacagccgc tgcggaaggt gaaacaatgc
    20281 ttacagtcat aaatggattc cttatgacaa attggttaac aatgccgtca aaattggtcg
    20341 cacaattctt acggcgtgga ttaagcaggt gcctgacctg cttttttcat atctgcatct
    20401 gacgctttcc ggtgttttcc tccttctgtt cctgcgttct cattacctgt cagccggatg
    20461 actgacagaa caaataaatg cgtattgccg gagcctgaaa taaatcctga tttttaaaga
    20521 acatcacccg ttgagtttcg ggagccgcgg ttttgacgtt tcgcgggaac ctgctgctgt
    20581 attcactgtg gtatgaactt cacccgtttg agaaccttcg ccgcaagggg catcactgcc
    20641 acggcctgac gcacctgtcc tgggcagaac gcaaaatttt ttaccgggtt ttgggtaaaa
    20701 aattttgtgg gctgttcatt taccctggac agtcagtgcg gcaggttgtg gctgtatcag
    20761 cccccggcga cagggactca tacgggtgtg tggcacccgg caggcaatga gcaggggccc
    20821 gccgcaacga cggagagcgc gtgcgcgaaa cggcgttgcc ccttacatcc cggcggcctt
    20881 ttgccgggat gccgggagcg caggccacgg cgcgacagag gcggcgcgac cggaacggtc
    20941 gctgcaagcc aggggttttc acgcatcgga ccgtagcggc agcccgaaac cgcgccagcg
    21001 ggttcggcgg agactggcag gtgcgccagc agctgcctgt cggagtcggg cctgacgacc
    21061 ggatgccgcc aggcagccgg gagccgaagg tgcgcccggc agacagaaca acactgcagt
    21121 agtcagaatc agagcagtag ataaaggaac aacaacatca ggaacagttc cataacataa
    21181 gaaagaaaca ctcctgagtc agacacatca cagacgggaa gggtaacggg cgacgcgcca
    21241 gcgtggcccg cttcccttcc ggcaggcgtc agcctgccag gggttttcgc gcgggctgac
    21301 aggcggcctc caccgcctgc ccctgtatca cctgtttatg ctgccctgaa ggatgtagtg
    21361 accgccgagg aatacagcct gctgattcgc cacaaaatcg cagatttcat caacaatctg
    21421 cgcagtggtg agcggtttct gccccatttc acgacgccgc gcattaatca cggctttgta
    21481 attgcgcaga atttcgcggg tttctgtgct gatattcatg acatgtgctc cccgtccagc
    21541 gcctgtaaca caacgtccat tgcttcgcgt tcttccctga cgaatgcgtc accgtcgccc
    21601 ccgttctcgc tcccgacggc atacaggcgc ttcatttcac tgcctgccag cgccatcact
    21661 ttttccagca tcaccgccgg aactgtcacc tgacggctgg cgcgcttttc ttccatcagc
    21721 tcacgaacaa cgtcgtgaac aatgctgaca tccgcaccat gcgcaaaggc atcgtccagt
    21781 cgctgcatga cggtttccag ctcatcgtct gtcagactcc agtcctgggc tatgtgacgc
    21841 acatccccac gggtcagcac cacggcaaaa acgcattcac tgtccggcac gtctgtgttc
    21901 agcacttcgc ggataatttc tttaacagta cagtacatgt tctcgtctcc tttacagggg
    21961 cggcattgcc gccccgtccg ttaatcagtc cagtgccgcg taaatcgcgt tgcattcggg
    22021 gtgaaattcg atgtgacgcc acaactgcgc atcgcgcatc cggtaaagct gggtcagtcc
    22081 tgcatccccg ctgtcgtggt acagccacaa ctggcggtta atcacaaggg aagtcagaat
    22141 gatgcctgcg gcatcagcac tcacggtacg gtcaaaccag tttgcaccgt tcaccatgtg
    22201 gaaacggtcg ccatccggca tcatgtaacc gccaccgtcc ggcaggcgga tgaagtgcca
    22261 gaagcctccc tgatattccg gcatcatttt ttgcgccagc gcgtacacat acgcctcggc
    22321 atacagaaaa tcgttgccaa acagatacgg tagaaaggag agacggtcgc actcttcaac
    22381 gttcagagtg acaggtttcg catattgcat gatgttttcc tccgttgttc acatattccg
    22441 cgacaccggg cggtgccgtg cctccataaa agccgggcga taaggcgggg atgcacaggg
    22501 cgcaagggaa gccgcagcct gacgcacacg gctggcggga gttggagggt gagaccgcga
    22561 aatctgagcg ggtgaacgac cggaaaatca cggcagacgg gaaggaggga ccgggtgacc
    22621 gcagcggcga agcgtaccct tgcatccctg ccagcccggc taagatggag gcacggcaca
    22681 gcccgcacac gacgctgagg ccacataact gaaaaaccac aaggccatgc cctgcatggc
    22741 cgggtaaagc ggagccggcg gcgacttcct ttgtgctctg tccggggacc ggcaggaaaa
    22801 aggcggggat ttaccccgcc aacgtcatca tttccggatt tttgccaggg catccgccat
    22861 gctgcggatt tcggcccggg cttcccggac cacctcccgc agaacaccac ggtcaaacca
    22921 gcggcggggc ggttcatccg gtatacgcag cacatcgcag gccagcccac tggacaggat
    22981 atgctcaata caatcgctga ccaggtacat ttccaccgac gaaaaacgtg ctccccggtt
    23041 cacgatggca tcgagcacca cgggcaggtt ttcgtcatct tcatcgagcc agcaggggtg
    23101 atcgctttcc ttcacaacca gacgaaagcg gcgcgtctgg cgagtcagca acgtctgacc
    23161 ggtggtggtc agcgccgccc ggatacgccg cgcatcttct gccacccggc aactgacact
    23221 gcgtaccgca tcgcgcaccg ccgcaagtac agccctgatt tcgtcttcgg tcagccattc
    23281 tccggcatac gggaaagaca cctgcgttcc gtctgccgga agtaccggca gcgactgcca
    23341 ctgactgccg gacagtacca gttccgcccc ggttccggtc agtttctgaa tggcggaggc
    23401 caggagcggc ggcagtacgc cgccgtgacg gttgataagt aacgtcgcca gccggggcgc
    23461 atccagcaga acataaatgc tgatccagcc cggatacttc gggttacgct ggtcaacatc
    23521 cgtctcttcc ggccagccct gcgccagcag caggttgcgg gcgtgacgac aaatcagtgc
    23581 gttcagcgtg ctgttttcgt tcatgattaa gcgttctccc tgtatcgccg cccgcagggc
    23641 aggcggcgat aacatcagtc gttttcgtgt gtgttgtttt tcagcagggt gcgggcctga
    23701 ttaaccacct cctgacggcg ggtgacgcat tccgggacgg cgaggccatg atcccgggcg
    23761 gcccattcac gtttccacaa tgcctgttca gcggtctgaa tcagggacgc cagcagttca
    23821 gcagggacgg tcaccaggcg gttttcgctc tcgttgcgga taatctccat caccgcagcg
    23881 gaagaaatac cggactcaat ccgctggtct tccgggatgt cctccaggcg tgccaggacg
    23941 gtcctgattt catgaccggt cagagaaatt tccataccgt cagccagtgc ctgaatctct
    24001 tccggggacc agataaccag catcagcggc atgtctcccg gatactttac tgccagctcc
    24061 cggcatagcg tttcgcatgt tccgtacatt ctcgtctcct tctcaatctt taaagcacgc
    24121 ttccagttcc agaatttcgg tatatgcctc catcaggagg cggtttgcgc gaaacagcgt
    24181 ctgacgcacc agacgttccg gcagggtgtc ggcattaaag ctgtaccgct gctccatctc
    24241 attcatggtt gtacgcaggt cgcacatggc attggccacc tccgccactt cggcgcgcag
    24301 tttttcgtgt cctgcatagt tcatttacgc agcctccggc ataaaccagt catcattcgc
    24361 ggccccctgc tgcatggccc gttgcacggc ggcaaggcgt tgctgcccgg cacggtgata
    24421 ctgctcaagc agctcgatac cgatataccg gcgtccggac tggagggcgg cgacgcaggt
    24481 tgagccgctg cctgcaaacg ggtccagcac aattgcgttc gggtgtgtga agctctcaat
    24541 cagcggttgc aggctggtaa cgggcttttc cgtcgggtga tggcgattgc ccgaatattt
    24601 ccagcccagc acgtccggca gcgggttttg tggcagacgt ggacggcctt ttgccaggat
    24661 gtaggcacat tcgtggcgat agcccacata tgcggccttc gatgtgtagt ttttggtgaa
    24721 gaccaggtga ccaacaacgc tgaatcccgc atttttccag gcggccataa agcgatcgac
    24781 gcggttccag ccatagaagc tcaccattaa cgcgtctttt ttgagtacgc ggtacatttc
    24841 attacaggct ggttgcagcc attcatcggt tttatcgccg gcgatggtgc gcccggaacg
    24901 gtcacggaaa ccgacgagat acggcgggtc ggtgaggatg aaatcgacag cattgcccgg
    24961 aaacgtggcc ataacacgca cgcagtcacc caggataaaa cgggacataa cagcactcct
    25021 tctcttacag agggcaccgg gagggcaccc ctgtggtgcc cgttccccgc tgcctgtggg
    25081 ctgttcaccg cgtccggtaa cgggtcaggc cgaccgtgga ataccgcagc cgtcgtcttt
    25141 ttgacggcga ggatatgccg cgaaagcggc ttgaagggcg ttcatcagaa aagggcgacg
    25201 ggctggcgta cactgtcacc aacgccagcc gggagcggta aaagcgcccg tcccgtgccc
    25261 ggccggacgc gggacactga ccacaggcag cgttcctgcc gcgccgcgtc agccggcgca
    25321 gtaaaaagag gagcccggcg acatcttttg ttacccgcca aacaaaaccc aaaaacaacc
    25381 catacccaac ccaataaaac accaaaacaa gacaaataat cattgattga tggttgaaat
    25441 ggggtaaact tgacaaacaa acccacttaa aacccaaaac atacccaaac acacaccaaa
    25501 aaaacaccat aaggagtttt ataaatgttg gtattcattg atgacggttc aacaaacatc
    25561 aaactacagt ggcaggaaag cgacggaaca attaaacagc acattagccc gaacagcttc
    25621 aaacgcgagt gggcagtctc ttttggtgat aaaaaggtct ttaactacac actgaacggc
    25681 gaacagtatt catttgatcc aatcagcccg gatgctgtag tcacaaccaa tatcgcatgg
    25741 caatacagcg acgttaatgt cgttgcagtg catcacgcct tactgaccag tggtctgccg
    25801 gtaagcgaag tggatattgt ttgcacactt cctctgacag agtattacga cagaaataac
    25861 caacccaata cggaaaatat tgagcgtaag aaagcaaact tccggaaaaa aattacatta
    25921 aatggcgggg atacattcac aataaaagat gtaaaagtca tgcctgaatc tataccggca
    25981 ggttatgaag ttctacaaga actggatgag ttagattctt tattaattat agatctcggg
    26041 ggcaccacat tagatatttc tcaggtaatg gggaaattat cggggatcag taaaatatac
    26101 ggagactcat ctcttggtgt ctctctggtt acatctgcag taaaagatgc cctttctctt
    26161 gcgagaacaa aaggaagtag ctatcttgct gacgatataa tcattcacag aaaagataat
    26221 aactatctga agcaacgaat taatgatgag aacaaaatat caatagtcac cgaagcaatg
    26281 aatgaagcac ttcgtaaact tgagcaacgt gtattaaata cgctcaatga attttctggt
    26341 tatactcatg ttatggttat aggcggtggc gcagaattaa tatgcgatgc agtaaaaaaa
    26401 cacacacaga ttcgtgatga acgttttttc aaaaccaata actctcaata tgatttagtt
    26461 aacggtatgt atctcatagg taattaatga tggacaagcg cagaaccatt gccttcaaac
    26521 taaatccaga tgtaaatcaa acagataaaa ttgtttgtga tacactggac agtatcccgc
    26581 aaggggaacg aagccgcctt aaccgggccg cactgacggc aggtctggcc ttatacagac
    26641 aagatccccg gacccctttc cttttatgtg agctgctgac gaaagaaacc acattttcag
    26701 atatcgtgaa tatattgaga tcgctatttc caaaagagat ggccgatttt aattcttcaa
    26761 tagtcactca atcctcttca caacaagagc aaaaaagtga tgaagagacc aaaaaaaatg
    26821 cgatgaagct aataaattaa ttcaattatt attgagttcc ctttatccac tatcaggctg
    26881 gataaaggga actcaatcaa gttattttct taccagtcat tacataatcg ttattatgaa
    26941 ataatcgttt gcactgtctc tgttattcag gcaatttcaa taaaggcact tgctcacgct
    27001 ctgtcatttt ctgaaactct tcatgctgca tttcgatttt tcttctaaaa tcataacttg
    27061 catacctgtt ctttttatgt atatccagat ctatttttgt gacgagttca aaccacaatg
    27121 atagcactcc ccatgcagcg ttatgaaaca tttccgcaac agatttagag tctgtattaa
    27181 ccgcttcagt catgtagtcg ctaatacgtt ctgcagtgat tttcgttatt tcatcgtaat
    27241 tcattttatc ccctacaaga ctatttaatg gaatgaaaaa tatttggtct gataacaatg
    27301 ctcctgaatt aacacctgca ggctatgtta aagttgccct cacacacaat ctacctgttt
    27361 tctcgtgtgt gtcacaggcg caatacataa atagtctggc cattaagttc gtaacggatt
    27421 tcgtactcgc cagcaaaaat ccgtctgaca tccctggttt caaactgaaa caactgttca
    27481 tcccatacgt ggatgagtta acagaatgac cggcgcctgt gtcagggact acaccgtttt
    27541 ggctgcagct ggtttactga ccaacgccag aaaatcatat aactgcgtca atgtcagcgt
    27601 atccagcgcg actgtattat tttt
//
LOCUS       DAEBXN010000050        27601 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.050, whole genome shotgun sequence.
ACCESSION   DAEBXN010000050 DAEBXN010000000
VERSION     DAEBXN010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27601
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.050"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            319..1617
                     /gene="kgtP"
                     /locus_tag="KJL99_002186"
     CDS             319..1617
                     /gene="kgtP"
                     /locus_tag="KJL99_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HBD4436906.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1614..1937)
                     /locus_tag="KJL99_002187"
     CDS             complement(1614..1937)
                     /locus_tag="KJL99_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HBD4436907.1"
                     /translation="MRILFVCSLLLLSECSHMDNDSWSGQDKAQHFIASAILSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="KJL99_002188"
     CDS             complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="KJL99_002188"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HBD4436908.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIRERTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3452..6112)
                     /gene="pat"
                     /locus_tag="KJL99_002189"
     CDS             complement(3452..6112)
                     /gene="pat"
                     /locus_tag="KJL99_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HBD4436909.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRNHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLARREES"
     gene            complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="KJL99_002190"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="KJL99_002190"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HBD4436910.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="KJL99_002191"
     CDS             complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="KJL99_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HBD4436911.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7537..8574
                     /locus_tag="KJL99_002192"
     CDS             7537..8574
                     /locus_tag="KJL99_002192"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HBD4436912.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENSCQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8622..9311)
                     /gene="ung"
                     /locus_tag="KJL99_002193"
     CDS             complement(8622..9311)
                     /gene="ung"
                     /locus_tag="KJL99_002193"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBD4436913.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAECE"
     gene            9616..9999
                     /gene="grcA"
                     /locus_tag="KJL99_002194"
     CDS             9616..9999
                     /gene="grcA"
                     /locus_tag="KJL99_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HBD4436914.1"
                     /translation="MITGIQITKAANADLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="KJL99_002195"
     CDS             complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="KJL99_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HBD4436915.1"
                     /translation="MTPILLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10745..11626
                     /locus_tag="KJL99_002196"
     CDS             10745..11626
                     /locus_tag="KJL99_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBD4436916.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQALNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPHFAVEKEIESGELIELPFGEQSQTITAMCAHYAGKAVS
                     PAMHIFIQCVEECFVVA"
     gene            complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="KJL99_002197"
     CDS             complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="KJL99_002197"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HBD4436917.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELHPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13127..13864
                     /gene="trmN"
                     /locus_tag="KJL99_002198"
     CDS             13127..13864
                     /gene="trmN"
                     /locus_tag="KJL99_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HBD4436918.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="KJL99_002199"
                     /pseudo
     CDS             complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="KJL99_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
     gene            15727..15882
                     /gene="rseD"
                     /locus_tag="KJL99_002200"
     CDS             15727..15882
                     /gene="rseD"
                     /locus_tag="KJL99_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HBD4436919.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            15879..16454
                     /gene="rpoE"
                     /locus_tag="KJL99_002201"
     CDS             15879..16454
                     /gene="rpoE"
                     /locus_tag="KJL99_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HBD4436920.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16487..17137
                     /gene="rseA"
                     /locus_tag="KJL99_002202"
     CDS             16487..17137
                     /gene="rseA"
                     /locus_tag="KJL99_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HBD4436921.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQSATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17137..18093
                     /gene="rseB"
                     /locus_tag="KJL99_002203"
     CDS             17137..18093
                     /gene="rseB"
                     /locus_tag="KJL99_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HBD4436922.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18090..18569
                     /gene="rseC"
                     /locus_tag="KJL99_002204"
     CDS             18090..18569
                     /gene="rseC"
                     /locus_tag="KJL99_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HBD4436923.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18767..20566
                     /gene="lepA"
                     /locus_tag="KJL99_002205"
     CDS             18767..20566
                     /gene="lepA"
                     /locus_tag="KJL99_002205"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HBD4436924.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20582..21556
                     /gene="lepB"
                     /locus_tag="KJL99_002206"
     CDS             20582..21556
                     /gene="lepB"
                     /locus_tag="KJL99_002206"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBD4436925.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21828..22508
                     /gene="rnc"
                     /locus_tag="KJL99_002207"
     CDS             21828..22508
                     /gene="rnc"
                     /locus_tag="KJL99_002207"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HBD4436926.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22505..23410
                     /gene="era"
                     /locus_tag="KJL99_002208"
     CDS             22505..23410
                     /gene="era"
                     /locus_tag="KJL99_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HBD4436927.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGCDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23422..24150
                     /gene="recO"
                     /locus_tag="KJL99_002209"
     CDS             23422..24150
                     /gene="recO"
                     /locus_tag="KJL99_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HBD4436928.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGDTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDG
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24162..24893
                     /gene="pdxJ"
                     /locus_tag="KJL99_002210"
     CDS             24162..24893
                     /gene="pdxJ"
                     /locus_tag="KJL99_002210"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HBD4436929.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKIRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            24893..25273
                     /locus_tag="KJL99_002211"
     CDS             24893..25273
                     /locus_tag="KJL99_002211"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HBD4436930.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25693..25773
                     /gene="shoB"
                     /locus_tag="KJL99_002212"
     CDS             25693..25773
                     /gene="shoB"
                     /locus_tag="KJL99_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HBD4436931.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="KJL99_002213"
     CDS             complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="KJL99_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HBD4436932.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            26442..27538
                     /locus_tag="KJL99_002214"
                     /pseudo
     CDS             26442..27538
                     /locus_tag="KJL99_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
BASE COUNT         6808 a         6863 c         7235 g         6695 t
ORIGIN      
        1 taagcgaaag gccatccgtc aggatggcct ttttgcattg gcgcagaaaa aaatgcctga
       61 tgcgacgctg cgcgtcttat actcccacat atgacagatt cagcaacgga tgcggtttcc
      121 ccaacttgcc tacttccata cgtgtcctcc ttaccagaaa tttatcctta agctcctcaa
      181 taaccatttt cctgctaact aaattcatgg tttaggttgc ataatgatac gcaacaaatg
      241 tataacattt tctttaccaa aagaaataaa caaaagcgac caacaaaagc atcggattac
      301 ggcaggagac ataatggcat ggctgaaagt actgtaacgg cagacagcaa actgacaagt
      361 agtgatactc gtcgccgcat ttgggcgatt gtgggggcct cttcaggtaa tctggtcgag
      421 tggttcgatt tctatgtcta ctcgttctgt tcactctact tcgcccacat tttcttccct
      481 tccgggaaca cgacgactca actactacaa acagcaggtg tttttgctgc gggattcctg
      541 atgcgcccaa taggcggttg gctatttggc cgcatagccg ataaacatgg tcgcaaaaaa
      601 tcgatgctgt tatcggtgtg tatgatgtgt ttcggatcgc tggttatcgc ctgcctccca
      661 ggttatgaaa ctataggtac gtgggctccg gcattattgc ttctcgctcg tttatttcag
      721 ggattatccg ttggcggaga atatggcacc agcgccacct atatgagtga agttgccgtt
      781 gaagggcgca aaggttttta cgcatcattt cagtatgtga cgttgatcgg cggacaactg
      841 ctagccctac tggttgtcgt ggttttacaa cacaccatgg aagacgctgc actcagagag
      901 tggggatggc gtattccttt cgcgttagga gctgtgttag ctgttgtggc gttgtggtta
      961 cgtcgtcaat tagatgaaac ttcgcaacaa gaaacgcgcg ctttaaaaga agctggatct
     1021 ctgaaaggat tatggcgcaa tcgccgtgca ttcatcatgg ttctcggttt taccgctgcg
     1081 ggctcccttt gtttctatac cttcaccact tatatgcaga agtatctggt aaatactgcg
     1141 ggaatgcatg ccaacgtggc gagtggcatt atgactgccg cattgtttgt attcatgctt
     1201 attcaaccac tcattggcgc gctgtcggat aagattggtc gccgtacctc aatgttatgt
     1261 ttcggttcgc tggcagccat ttttaccgtt cctattctct cagcattgca aaacgtttcc
     1321 tcgccttatg ccgcttttgg tctggtgatg tgtgccctgc tgatagtgag tttttataca
     1381 tcaatcagtg gaatactgaa ggctgagatg ttcccagcac aggttcgcgc attaggcgtt
     1441 ggtctgtcat atgcggtcgc taatgctata tttggtggtt cggcggagta cgtagcgttg
     1501 tcgctgaaat caataggaat ggaaacagcc ttcttctggt atgtgacctt gatggccgtg
     1561 gtggcgtttc tggtttcttt gatgctacat cgcaaaggga aggggatgcg tctttagtga
     1621 cgggtcagtt gccagacggt atagccggtg cttgcaccgg cgacatccca ggccaaatcc
     1681 ttccagctcc agccgctccc ttcggggcgg ctatcccaaa gctcttttga cgcccccaaa
     1741 ctgacagaga acatcaatcc aaacatggca ctgcgatccc ggctcatccc ctgatgctgt
     1801 gaatattcat ttccggcggc ggaaagtatc gccgaggcga taaagtgttg agctttatcc
     1861 tgcccactcc agctatcgtt atccatatgg ctgcattcag aaagtaacaa tagcgaacag
     1921 acaaaaagaa tacgcaaaac gtggcctcca aaaaacaaac cccgtacagg acggggttgt
     1981 gattacagga tgcggctaat taatcggtcg atgcggatac ggcgcaaacg gcggatgagt
     2041 ttacgaacct tcaccggata atcggcaata ctttgcagat cgcgatagtg cttaacgatg
     2101 gtggtacgct cgcggatcag ctccagttct ttctctcgct gcggcgcaag ctccagttgt
     2161 ggatcgtgga tcaaaatggc gttttccaga tccagacgcc aggcgcgcgg gttcaggtta
     2221 ttaccggtga tcaacatcca cttatcatca acccacatcc ctttcaggtg ataggtgttg
     2281 tcgtcatctt tccataaccg aaccactagc tggtcagtat tgacgtaata ctgcaaacgg
     2341 ctcaggaaac gacgcagatt gatctcatag agataaggca atgcgccaat tatcttgaaa
     2401 ggttcatctt ccggaatgta gaagtcgttc gcggttttat caccaacaat aatttcgacc
     2461 tttttccctt cgcgcagcaa ctggataata ttgcgcacaa ggattgctgg caggttgaag
     2521 tatggcgtac agatggttag tttctgctcg gcacaaggca taagatggaa aatggtcttg
     2581 ttcaacagac tcgatttccc cagccccact agcggcgtta cggaaagctg atcgttgtcg
     2641 gcatcgccct ggaaatgata agcggcatca cgcagctcct ggcggaacag acgaatatcg
     2701 ttcttgattt ccgggctttt tggccgatta acatcatcca gacgattaac gccgcggcca
     2761 ttcataatat tctgtgtaac ccattcaaac ataatgtctg acatcttacg gttacggatc
     2821 agatgataac ggtcgtagcg atatttatcg tgttgatgca ggtaaacatc gttcaggctg
     2881 gcaccgctat aaagtacgct atcgtcgatg ataaagcctt taaagtgcag aacaccaaga
     2941 gcttcacggg tattgattgg aacgccataa accggaacat ctacgcccgg attttcctgc
     3001 gccatgcggc agtaccagtc agcgttagtg ttagatgccg cagcgccaat gcgtccacgt
     3061 tgtgcacgat gccagtcgac cagcacccgc acatccagtt ccggacgctg ccttttagcc
     3121 tcatacaacg cgttcagaat gcctttgcca ccgtcatcct gttcgagata cagggcgaca
     3181 atgcaaatgc gctgcttcgc gctggctatt ttttccagca gcgtctcccg gaagtcggcg
     3241 ggagcgtaaa agaaatcgac atcatcaact gattgagaaa tcttgggtag ttgggcaagg
     3301 tgttgttgat gtttattacg cttaaatttt gacaacatca cagtgcattt cttctctgtt
     3361 cattgaaggg tcctctgtgc aatgcagacg acataagcgg gcaataataa caccagtccc
     3421 gattaagtgg tcaacatttc cagtacctta ctcatgattc ctcgcgccgg gcaagattta
     3481 gcgtaagccc aacgatcccc tcttcgagct ggatatcaac gttaaacccg agcttgcggg
     3541 ctagcgccac catgccacga ttgtttggca tcgtaatacc attcagacgt tgtagtccgt
     3601 gatttcgcgt ataggtaatc aacttttcca ttaagcgtcg acctaagcct aaccctttga
     3661 gatccgagcg aaccagtaca gcaaattcgg catcgatgtt atcaggatcg gaaatcgcac
     3721 gcgtgacgcc gaggatctct tccgtttgat caatacgtcg taccgctaca aacgccattt
     3781 cccgatcgta gtcgatctgt gtcatgttgg ctaaatcttc atgggtaaat tcgttgatct
     3841 cgctaaagta gcggtaataa agatcttctt tggtgactcg cgaaatgaat tgctgaagtt
     3901 gtggctcatc ttctggcaaa atcgggcgga acaagcagcg ttcaccgttt ttcaattcta
     3961 cccattcttc cagctgatgc ggataagggc gcactgccag ccgactctcg ttatcgcctt
     4021 caaacggcga gatatccagc gtgacatcca gcgcggtaaa ttcactgcca gaagccagca
     4081 aaggatgaat atccagacgc tgaatttccg ggcaatcgac aatcaagttg gaaacctgca
     4141 ccagaagctg gctcaagcct gcaacatcca atgggcgtag cgcactgcgc gcacgaatct
     4201 ttttactttt gatcccctga ataaccagat agcgggccag gttcatgttc agcggcggca
     4261 gtgcgacgac ggcttgatct tcaggacgcc actccacacc gccttcaccc agcatgatca
     4321 acggcccgaa aaccggatcg tgctcaacca caacccgcaa ctcctgagcg ccagcacggt
     4381 tagccatact ttgcaccaac aggccgtgga cccgcgcctg tggccaggcc atttttacgc
     4441 gatcgaaaat agcgttcgcc gcttgctgga cttcattggc tgtacgcagg taaagcatga
     4501 cgccctgaac ttccgattta tgtggaatat ccggcgaacg caatttcagc gccaccggat
     4561 aaccaatctg ttcggcaata tgcaccgctt cggtgctatc gctggcaatc caggttggga
     4621 gcgtgttcat gccatacgct tgcaggatgg gctgaacttc atgggtatcg agcgacgtag
     4681 ccccttcggc aatcgcctgt tgcaacagaa gatgcgcttc tgcggtattg gaagtcagat
     4741 tgctgggcaa cgccggcgtt tcgcgtagtt gcttctgatt acgccggtac tccaccatat
     4801 gcataaaagc agtgatggtt ccttccgggg tacggtaggt cggcagcccg gcttcgctga
     4861 ataaacgtcg tgcctcttgc gaggagtgct cgccgcacca gttcgtcagc aaagagacat
     4921 atttgctgcg gggatgatgc tttaccgctt caattaatac ttgcgcgctt tctgttgcgg
     4981 gagcagcggc gctgggcgaa tgaataacca tcagtgcatc aaaatcctgg ctgtggagca
     5041 gaatgtccag cgttttgaca taatgctcgc tgctggcgtc atcgcgcaga tcgagcgggt
     5101 tagagactgc cacatgttct ggcaatgcat cgcgcaattt ctggcaggtt tcttcgctta
     5161 gcgttgccag cttgccattg cgtgaccata aggcatccag cgccagcgcg gcaggcgcag
     5221 caccgttgct gataatcatc aaccggtcgc cacgcagcgg gcgcatatgg ctaagggttt
     5281 ccaccgccga aaacagttcg tgggtatcct gtacccgcaa caaaccggca cgctgaatag
     5341 ccgcatccca tgccgggtcc attcctgccg tggtgttgag cagtcgctgt gccgccgggc
     5401 tacgtccgct tttaatcacc agaatcggtt tattacgcga ggcactacgg gccgccgaaa
     5461 caaagcgtcg cgcgtcgctt aactgttcga gatagagcag gatggcgctg gttttactgt
     5521 cgcgcgccag atagtcgagc aattcatcaa cgtcgatatc taggctgtcg ccgagcgcaa
     5581 taaagtagga aaagcccatc tcacgctgtt gcgcccagtc gaggatggtg ttggagacgg
     5641 cagccgattg cgaaataaac gccagcttgc cgcgtttaat cggcacaggc gaaaagctgg
     5701 cattcagacc ttgccaggga gccagtaaac ccagactgtt tggtccaagc aggcgcatgt
     5761 tatggcgcag ggcgcaggcg cggagatctt cgtgttgcga tgccggggcg gaaagaataa
     5821 tgcaggtttt acagcctttc tcgccgagct cttccagaag agcaagatta cggctggcat
     5881 tggtacataa aaccgcaagg tcgggtgtaa agggcaagct ggcaatatcc ggccaggcca
     5941 acacacccaa caccgctttc caggctggcg tcaccgggag taccggtccg ttaaagcctc
     6001 ccgccagcag gttacgcatc atcaggtaac ctgcgcgatt gggtttcatc gacgcgccaa
     6061 ttaccgctat cgattttggt cgcagtagtg cttccagtcc tcgctgactc ataccggtct
     6121 cccgcagaag tgaccgaatg attttaaacg ctttctaact gttctgctgt gatgctaccc
     6181 agatgttgcg tttttcctgc cagatagcgt gttttaaagc gggtaaaatg ctcgcctaac
     6241 cctgctgccg ccccggtatc gccggccata tctaacagtg cgatggctac ctcggcagta
     6301 caatattggc ctgcagcctg ggcttcacgc aggcgatagg cagaaagccg ggaaagatcg
     6361 acggaaatga cgggaagatt atccagatac ggacttttac gaaacatctt gcgagcttcc
     6421 ggccaggtac catcgagcat gataaacagc ggtggcttac cggcaggtgg tgtgaagatc
     6481 acttcccgtt gctcatcagc atacgaggcg ggaaagacca ccattggctg ataatccggg
     6541 ttttgtacca gatccagcaa atcctgcgag ggttcggtac gcgaccattg aaacgcaacg
     6601 gtatcaggca aaatatcagc aatcagacgc ccggtattac tgggcttcat tggctcggtg
     6661 tcgaacatca gcaaacagaa gcgacttttt gcttgtgctg gggtaattgt cgaacagaga
     6721 cataatttct ctggcaaaag acagcgttgg cagcgacgaa cgcgattacc gcgggcaaga
     6781 aaaggacgtg ttgcgcgcgc aatacgctcg gcgcgtaact ggagaacagc gttttcggtc
     6841 ataagagagc gtcgaaaaaa cgccattgtc gcagaggaga aaacggggca caagatgcgc
     6901 cccggtaaga ttaaagagat tcgttcagcc agctatcgaa cggcgctttc ggtactgcgc
     6961 cattaagcat gtcgacaacc tgaccgtttt tgaaaatcat gatcgtcggt atactacgaa
     7021 ttccaaagcg actgctcaat tcacgttcag cttcggtatt cactttcaca aagcgcactt
     7081 taccgctacg ctcttgcgcg acatcttcaa aaattggtgc gaaattacgg caggggccgc
     7141 accacggtgc ccagaagtcg atcaccacag gtagatcatc cttcagcaat ttgtcgagtg
     7201 tttcaccggt cgcattaatc acctctccgt caaacaagtc gtgaccgcag cgtccgcatt
     7261 ttgccgcatc ttcgatccga tcgtcgggaa tgcgattgat ggcctgacaa tgggtacaaa
     7321 cggtattcat aactaacctc gggatgagta aggagactta tcggcaacga cgccggtatg
     7381 tttctaatat gttacatatt atcggggaca ttgttccaga caacaatctg ttttattcaa
     7441 taggtatgat agtcgcaggc tttgctacga aatcatggcg aaccgcgcgg acttcgggta
     7501 atctgcgcgc ttcgcgcagc gctggtggag aaaagcatga acgatgaaat gaaaggtaaa
     7561 agcggcaagg tcaaagtgat gtatgtccgc agtgatgatg attctgataa acgtacccac
     7621 aacccgcgta ccgggaaagg gggcgggcgt ccaggaaaat ctcgtgctga tggtggccgt
     7681 cgccccgccc gcgatgacaa acagagtcag ccccgtgacc gcaagtggga agattcgccg
     7741 tggcgcacgg tttcccgcgc gccgggtgat gagacgccgg aaaaggccga tcacggtggc
     7801 atcagtggta aaagttttat tgatccggaa gtgttgcgtc gtcagcgtgc ggaagaaacc
     7861 cgcgtctacg gcgaaaactc ctgtcaggca ctgttccaga gccgtccgga agcgattgtt
     7921 cgcgcctggt ttatccagag tgtaacgccg cgttttaaag aagccttgcg ctggatggca
     7981 gcaaaccgca aagcgtacca tgtggtggat gaagcggaat tgacaaaagc gtcaggtacg
     8041 gaacatcacg gcggcgtttg cttcctgatc aaaaagcgta acggtacaac cgtgcagcag
     8101 tgggtaatcc aggcaggcgc gcaggattgc gttctggcac tggaaaacga atctaacccg
     8161 cataacctgg gtggcatgat gcgcagctgc gcgcacttcg gcgtgaaagg tgtcgtggtg
     8221 caggatgcgg cactgctgga gtcgggggcg gctatccgta ccgcagaagg cggcgcagag
     8281 cacgttcagc cgattactgg cgacaacatt gttaacgtgc tggatgattt ccgtcaggca
     8341 ggttacaccg tagtgaccac ttccagcgag cagggtaaac cgctgttcaa aaccagtctg
     8401 ccagcgaaaa tggtactggt gctgggtcag gaatatgaag ggttaccgga tgccgcacgc
     8461 gatccgaacg atctgcgcgt gaagattgac ggtacgggca acgttgccgg gctgaatatc
     8521 tctgtcgcaa ccggcgtttt gcttggtgaa tggtggcgtc agaataaagc ctgataaatc
     8581 agccgggtgg caactctgcc atccggcatt tccccgcaaa tttactcaca ctctgccggt
     8641 aatactggca tccagtcaat cggcgtctcg ccacgttgtt ccagccactg atttgccagc
     8701 acaaaatggt tgcagccaaa gaatccacga tgcgccgaaa gcggcgacgg atgcggtgct
     8761 ttcagtacat gatggcgttg cttatctata atcgcccctt tcttttgcgc atgtgatccc
     8821 cacaacaaaa acaccacgcc ttcgcggtgc tggttaatca ggctgatgac tttatcagta
     8881 aacgtctccc aaccgaggct ggcgtgggaa tgcgcctgac ctgcgcgaac cgtcaacaca
     8941 gtattgagca gcagaacgcc ctgacgcgcc cagctttcga gatagccatg attagggcgg
     9001 gtgaagcccg gaatagtatt ttccagctct ttatacatat tcaataatga cggaggaatg
     9061 gcaatgccgg gacgaacgga aaatgccaga ccatgcgcct gtcccggtcc gtgataagga
     9121 tcctggccga gaatcaccac tttaacgtca cccaactcgg taaagcggaa cgcgttaaag
     9181 acatcttttt gtggtggata gatagtgacg ccggactgcc gctcgctggc gacggtctga
     9241 agggtattaa gaaaataagg ttgttgcttc tcttcagcca gcacgtcatg ccaggttaat
     9301 tcgttagcca tcttgctctc ctgcgaatct tcaatccgcc tagcttaact gcttctttct
     9361 acagaacaaa atcatgctca ttttttagac ttaaacgagt gcaaaaaaat gtaaattggt
     9421 aaaatttcac acccgcataa gttgatgtaa aacaataaaa tccatttacg accacaatcg
     9481 atgtgtggtt tttgttgatt taaatcaaag aatcaagggt ggttgaggag tatatataca
     9541 ctcaagcaac aatggtttta ccaattggcc gcgacaggct gaacaaatca aataattttg
     9601 ccggggaggc atcacatgat tacaggtatc cagattacta aagccgctaa cgccgatctg
     9661 ctgaactctt tctggctgct ggacagcgaa aaaggcgaag cacgttgcat cgttgcaaaa
     9721 gcaggttatg cagaagatga agtggttgca gtaagcaaac tgggtgacat tgaataccgt
     9781 gaagttccag tagaagtgaa accagaagtt cgcgttgaag gtggtcaaca cctgaacgtt
     9841 aacgttctgc gtcgtgaaac tctggaagat gcagttaagc atccggaaaa atatccgcag
     9901 ctgaccatcc gtgtatccgg ttatgcagtt cgctttaact ctctgactcc ggaacagcag
     9961 cgcgacgtta tcgctcgtac ctttactgaa agcctgtaag gcttaagtag caagttacta
    10021 agcggaagag ggcgacctct tccgcttttt ttcgttaata gaaaatgcgt accgcgcaat
    10081 agaccagcaa cagggcaagc acgatattta actggcgacc atactggcga aacaatcgct
    10141 gaaacagatg ccccgccagc gcccagcaca cattgccaaa cgtcccaatc atcgccagca
    10201 aaacgctgac gccaactacc cagcttagcg cctgtgtttg cggcagaaca aacgtcgaca
    10261 gtgccgtaac accgtacaaa atgattttga cgttcacaaa ctgcaaagca aagctggccc
    10321 aaaagctgat tggttttgcc tgaagtccgt cttcctttgt tgggctggtg gcgattttcc
    10381 acgccagcca gacaatatat gccgcccccg cccaactcaa aaggtgtacc gctgccgggt
    10441 caatcactgc cagtgaaaat gaaatgcccg cacacagtaa catcacaatc aaaaatccca
    10501 gactcatccc tgccagcacg cgggtacttt gacgaaatcc atgcgacgta gcagagctaa
    10561 gggcgagaat attgttcggt cctggcgtca tagcggtaat cagggtgtaa gtccaaaaag
    10621 cacttaaaag gatcggtgtc acttgttatc tcctctgttt tggtagacag tacccgacac
    10681 ggaacattta aaaaaatgaa tgtttttata ttctaaattc gatattttcg ataggtttgg
    10741 ggttatggat ctgcgccgtt ttattacgct taaaaccgtg gtggaagagg gttccttttt
    10801 gcgagcttcg caaaaattgt gctgtacaca atcgacggtg acttttcata ttcagcagct
    10861 tgagcaggag ttttcagtcc agttatttga gaaaattggc cgacggatgt gccttacccg
    10921 cgaaggaaaa aagttgctgc cgcacattta tgaacttacc cgagtgatgg atacgcttcg
    10981 tgaggctgcc aaaaaggagt cagatccgga cggggaacta cgcgttgttt cgggcgaaac
    11041 gttgctctct taccgaatgc cgcaggtgtt gcagcgtttt cggcaacgcg caccaaaagt
    11101 gcgtttatcg ttgcaggcgc tgaactgtta cgtgatccgt gatgcgctac tgaatgatga
    11161 ggccgatgtc ggcgtctttt atcgcgtagg gaatgatgat gccctgaacc gacgagagtt
    11221 gggtgaacaa tcgctggtgc tggtggcttc accacaaatt gcagatgtcg attttactga
    11281 gccgggaaga cataacgcct gtagctttat tatcaacgaa ccgcaatgtg tcttccggca
    11341 gatatttgag agcacgctgc gacagcggcg gatcacggtg gaaaacacca ttgagcttat
    11401 aagtatcgaa agcatcaagc gttgtgtcgc ggcgaatatc ggtgtcagtt acttgcccca
    11461 ctttgcggtg gaaaaagaga ttgagagcgg agaattaatt gaattgcctt ttggcgaaca
    11521 gtcgcagacc attacggcaa tgtgcgcaca ttatgctgga aaagcggtta gcccggcgat
    11581 gcacattttt attcagtgtg ttgaagagtg ttttgtggtt gcgtaaaaaa acaatgccgg
    11641 gcttaaaccc ggcattgaga ttactcttct gtcgtttgcg gtggcacgcc cgttccgctc
    11701 ggtttacggc gcttaccaat atttttggtg tcgcgatggc gttttttcac ccgcggcttc
    11761 tctttctctt tagctttttt cttctcggcg cgttttgcca gtactttctt cgatggcttg
    11821 ccggtctgct tctcgcttgg cgcacgcgtt ttcgggtgta actcatcaat aacgcgagct
    11881 ttaatcggct cttcaatgta acggccaacc ttgcccagca gcaaatggtc atgggcttcc
    11941 accagcgaaa ttgcggtgcc tttacgaccg gcgcgcgcgg tacgtccgat acgatgcaaa
    12001 taagtatcgc cactgcgcgg catatcgaag ttaaagacgt ggctgacgtc aggaatgtcg
    12061 ataccgcgcg cggcaacatc ggtggcaacc agtacgttta cgcggccttc ggtcaggcgt
    12121 ttaattgctt cgttacgctt gccttgtacc atctcacctt cgagatagca gttgttgatg
    12181 cccgcttcgc gcagccagtt tgccagctca tgcacacgtt cacgcttacg cacaaacacg
    12241 attgagcgag tcgcttcagg ctgttttaac aggtgcacca gcaatgcggt tttatgttcc
    12301 aggtcatcgg cgcggtaata ccactgatga atttttttgc gctcacgggt ggacggattg
    12361 gcagaaactt ccaccggatc ttccagcaga cgctcggcaa agtcctgaat cgcatcgcct
    12421 tccagcgttg ccgaaaagag cagggtctgt ttacgccagc gagtttcgcc agcaatatgt
    12481 tcgatatcct gagcgaagcc catatccagc atacggtctg cttcgtcgag gatcagcgtt
    12541 tcaaccgcgc ggcaatcgaa gttctcttct tttatgtatt gcagcagacg tccggtcgtg
    12601 gcgaccacga tgtcctgatt ttcgctgaac acttccgcgt ggttcatata ggctacgccg
    12661 ccggtgatgg tggcgatatc cagatgcgta tgtttcgcca gttcgcgggc atgatcggcc
    12721 acctgcatcg ccagctcgcg agttggggtg aggatcaaaa tacgcggcgg accggatttc
    12781 ttacgtggga aatcgagcag gtgctgcaac gctggcagca gatacgccgc cgttttaccg
    12841 gtgcctgtcg gcgcagaacc gagtacatca cggccatcga gcgcaggcgg aatggcggca
    12901 gcctgaatgg cggtcgggcg agtgaaacct ttatcctgga gggcttccag caggctttcg
    12961 tcgagttcaa gttcggaaaa agtcgttaca gtcatgttct acctctgtgt ggggcgctga
    13021 ttatagacgt tacggctgca atcttcatct gtttgtatgg atatcgcttc tcgggtattg
    13081 ttctcacccg gtgctatcgc cgtttttcct gcaaggtttt attttcatgt cacagtctac
    13141 atccgtgctt cgtcgtaatg gatttacttt taaacagttt tttgttgctc acgatcgctg
    13201 tgcgatgaaa gtgggaacgg atggcatttt gctgggcgca tgggcaccgg tggctggggt
    13261 gaaacgttgc cttgatatcg gcgcgggtag cgggttgctg gcactgatgc tggcgcagcg
    13321 aaccagtgac agcgtgataa ttgatgccgt tgagctggaa agtgaagctg ccgcgcaggc
    13381 gcaggaaaat atcaaccagt ccccgtgggc agagcggatt aacgtccata cggcggatat
    13441 tcagcagtgg atcacacagc agacagtacg cttcgattta atcatcagta acccacctta
    13501 ctatcagcag ggagtagagt gcgcgacacc tcaacgggaa caggctcgct ataccactac
    13561 gcttgatcac ccatcgctgc tgacctgtgc ggcggagtgc attaccgaag agggattttt
    13621 ctgcgtggtg ctgccagagc agattggtaa tggttttact gaactggcat taagcatggg
    13681 ctggcattta cgtttgcgga cggatgtggc ggaaaacgaa gcgcgactgc cgcatcgggt
    13741 gctgctggca ttctccccgc aggcgggaga atgctttagc gatcgcttag tcattcgtgg
    13801 gccagaccag aactattccg aagcgtatac ggcgctgacc caggcttttt atctgtttat
    13861 gtaatgattg ccgggggaaa ggatcgatgg accggaatgc gtgagcagtt ccgggtaatc
    13921 cagcgtaaaa tgcagccccc ggctttcttt gcgcatcatc gcgcagcgaa caatcaactc
    13981 ggcaacctgt accagattac gcagctccag caaattattt gagacgcgga aatgggcgta
    14041 atattcgtct atttcttgtt ggagcatggt tatccgccgc agggcgcgtt ccaggcgctt
    14101 cgttgtgcgc acaatgccaa cgtaatccca cataaacagc cgtagctcgt gccagttatg
    14161 ctgaattact acccgttcgt cagggttctc aacgcggctt tcatcccacg gcggtaacgt
    14221 actgacgccg tgggcataag gcatacgtct ggtgatatct tctgccgccg accagccata
    14281 gaccagacac tccagcaatg aattcgaggc catgcggtta gcgccgtgta agccggtata
    14341 actcacctcg ccaatggcat acaagccctc gacgtccgta cgcccatgat catcaaccat
    14401 tacaccacct caggtatagt gtgcagcagg cacaatcggt accggttctt gtgtgagatc
    14461 aatccccagc ccgagcaatt tttcataaat catcgggaaa tgctggcgaa taaaatcggc
    14521 gggcttatgg ctgatgtcga ggaacataca atctgcgccg aggcgtttca tttcatggtc
    14581 aatggcgcgg gcgacaatat cgcgcggggc cagttcgccg cgctcatcaa aatcgggcat
    14641 aaaacgcgtg ccatccgggc gcttgagata agcgccttcg ccgcgcagtg cttccgttaa
    14701 caggaaattg cgtgcctgtg ggtgatacag tgcggtaggg tggaactgat taaattcgag
    14761 attggcaacc cgacagcccg cgcgccacgc catagcaatg ccatcgccag aagaaatatc
    14821 cggattggtg gtgtactgat acaccttcga cgcaccgccg gttgccagta ccactgcttt
    14881 tgcgtggcag gtttccaccg tttctttatt tcggttccat acccacgcgc ctacaacccg
    14941 tcgcgtgccc ggcaggccaa ttttgtcaga gacaatcaga tcaactgcgt tgctgcgctc
    15001 cagcacacga atattcggat ggttctgcgc cttgctcacc agcgtggatt gtacttctct
    15061 accggtggcg tcggcggcat gaagaatacg acggtgacta tgtccacctt cacgggtcag
    15121 atggtagctt tcttcgccat tcggttgaac gtgggtatca aacaaaaccc cctggtcaat
    15181 taaccatggc acacaggaac gagcattgct ggcgacaaat tcaactgcat ggtgatcgca
    15241 aataccagcc ccggcaatca atgtgtcttc cacatgcgag tcaatgctgt cagtttcatc
    15301 aaacacggcg gcaataccgc cctgggcata aaatgttgaa ccttccgtta ccgggccttt
    15361 acttagaacg atgacctgat gctggtcagc caggcgtagc gccagtgaaa gtccggctgc
    15421 gccgctacca ataatcaaca cgtcacatga atgttcaggg agagtattca ttttctttgt
    15481 ttaatttact aaacattgtt tggtcagcat agcatcatgt tgtgcgaata aacacctgct
    15541 attttaatat ttgttacagt ggctaaacac gttgacgcag ggcggcgaga aaaagagaag
    15601 ttactggctg gtggaggatt aggtggtgaa ataaaaaggc cgttgggtta ctcttcaggc
    15661 agttaaatgg gcatttctac acagataatg cgatgttcag attctgtaga cttataatga
    15721 tagataatga tccgtctaca gcatgacaaa caaaaacaga tgcgttacgg aactttacaa
    15781 aaacgagaca ctctaaccct ttgcttgctc aaattgcagc taatggagtg gcgtttcgat
    15841 agcgcgtgga aatttggttt ggggagactt tacctcggat gagcgagcag ttaacggacc
    15901 aggtcctggt tgaacgggtc cagaagggag atcagaaagc ctttaactta ctggtagtgc
    15961 gctatcagca taaagtggcg agtctggttt cccgctatgt gccgtcgggt gatgttcccg
    16021 atgtggtaca agaagctttt attaaagcct atcgtgcgct ggattcgttc cggggagata
    16081 gcgcttttta tacatggctg tatcggattg ctgtaaatac agcgaaaaat tacctggttg
    16141 ctcaggggcg tcgtccacct tccagtgatg tggatgccat tgaagctgaa aacttcgaaa
    16201 gtggcggcgc gttgaaagaa atttcgaacc ctgagaactt aatgttgtca gaagaactga
    16261 gacagatagt tttccgaact attgagtccc tcccggaaga tttgcgcatg gcaataacct
    16321 tgcgggagct ggatggcctg agctatgaag agatagccgc tatcatggat tgtccggtag
    16381 gtacggtgcg ttcacgtatc ttccgagcga gggaagctat tgataacaaa gttcaaccgc
    16441 ttatcaggcg ttgacgatag cgggatactg gataagggta ttaggcatgc agaaagaaca
    16501 actttccgct ttaatggatg gcgaaacgct ggatagtgag ctgcttaacg aactggctca
    16561 taacccagaa atgcagaaaa cctgggaaag ctatcactta atccgtgatt caatgcgggg
    16621 tgatactccc gaggtgctcc atttcgatat ctcttcacgc gtgatggccg ccattgaaga
    16681 agagccagta cgtcaatcgg cgacattgat cccggaagcc cagcctgcgc cgcatcaatg
    16741 gcagaaaatg ccattctggc agaaagtacg tccgtgggcg gcacagctta cccaaatggg
    16801 cgtagccgca tgcgtatcgc ttgcagttat cgttggcgtc cagcactata atggacaatc
    16861 tgaaacgtcc cagcagcccg aaacgccggt atttaataca ctgccgatga tgggtaaagc
    16921 cagcccggta agcctgggag taccttctga agcgaccgca aacaatggtc aacagcagca
    16981 ggtacaggag cagcgtcgtc gcattaatgc aatgttgcag gattacgaac tgcaacgccg
    17041 actccactct gaacagcttc agtttgagca ggcgcaaacc cagcaagccg ctgtacaggt
    17101 gccaggaatt caaactttag gaacgcaatc gcagtaatga agcaactttg gtttgccatg
    17161 tcattagtga caggtagcct gttattctct gctaacgcct cggccactcc cgcgtccggg
    17221 gcgttattac agcagatgaa cctggccagt cagtcactga attacgagct gtcattcatc
    17281 agcatcaata aacagggtgt tgagtctctg cgttatcgac atgcacgcct cgataaccgt
    17341 cctcttgcac aattgttgca aatggatggc ccgcgccggg aagtggtaca gcgcggcaat
    17401 gaaatcagct attttgaacc gggacttgaa ccgttcacgc ttaatggcga ttacattgtt
    17461 gattctctgc catcgcttat ctataccgat ttcaaacgcc tttctcctta ctacgacttt
    17521 atctccgtcg ggcgcacgcg tattgctgat cgtctttgcg aagtcattcg cgtggttgcc
    17581 cgagatggta cacgctacag ctacatcgtg tggatggaca ccgaatcgaa attaccgatg
    17641 cgggttgatc tccttgatcg cgatggtgaa acgctggaac aatttcgcgt gattgctttt
    17701 aacgtcaatc aggatatcag cagcagtatg cagacgctgg cgaaggcaaa tttgccgccg
    17761 ttgctttctg ttcctgtagg tgaaaaagct aaattcagct ggacgccaac ctggttgcca
    17821 cagggtttta gcgaagtttc cagtagtcga cgtccgctac cgacgatgga caacatgcct
    17881 atcgaatcac gtctctattc cgacggatta ttcagcttct cggtaaacgt taaccgcgct
    17941 acgccatcga gcaccgatca gatgttgcgc accggacgca gaactgtcag tacaagcgta
    18001 cgtgataacg ccgaaatcac cattgtcggt gaactgccgc cgcaaacggc gaaacgcatt
    18061 gccgagaata ttaagttcgg ggcagcgcaa tgatcaaaga gtgggctacc gtcgtctcct
    18121 ggcaaaacgg gcaggcgctg gtcagttgtg atgttaaagc ttcatgcagc agctgcgctt
    18181 cacgcgccgg ttgcggtagc cgcgtgttga ataaacttgg cccgcaaacc acgcatacca
    18241 ttgtcgtacc ctgtgatgag ccgttagtgc cggggcaaaa agtggagttg gggatcgccg
    18301 aaggcagcct gcttagctcc gcattactgg tttatatgtc gccgctggtg ggattattcc
    18361 tcatcgcttc gctatttcag ctactctttg cttctgacgt cgcagcatta tgtggtgcgg
    18421 tcctcggcgg tattggcggg ttcctgattg cgcgcggcta ctcgcgtaag tttgctgccc
    18481 gggcagaatg gcaaccgatc atcttaagcg tggcattgcc gcctggcctt gtgcgatttg
    18541 aaacatcttc ggaagacgcg agccagtgat ttcactggct ttgttgcatt ttgcataaca
    18601 aatgcgagga cgtttccaga taccacaatc catacggtat gaacatttcc tcgcctcaat
    18661 gttgtagtgt agaatgcggc gtttctatta atacagacgt taagctcaga acagcgactt
    18721 ctaaaagcct ggttaaccgg gcattaaggc acaataatca tactttatga agaatatacg
    18781 taacttttcg atcatagctc acattgacca cggtaaatcg acgctgtctg accgtattat
    18841 ccagatctgc ggtggcctgt ctgaccgtga aatggaggcg caggttctcg attccatgga
    18901 tcttgagcgt gagcgtggca ttaccatcaa agcgcaaagc gtgacgctgg actacaaagc
    18961 gtctgacggc gaaacctatc agcttaactt tatcgacacc ccgggccacg tagacttctc
    19021 ctatgaagtt tcccgttcgc tggctgcctg tgaaggtgca ttgctggtgg tcgacgccgg
    19081 gcagggcgta gaagcgcaaa ccctggcaaa ctgctacacc gccatggaaa tggatctcga
    19141 agtggtgccg gtactgaaca agattgacct gccggcagcc gatcctgaac gcgtggcgga
    19201 agaaattgaa gatatcgtcg gcatcgacgc caccgatgca gtgcgctgtt cagcgaaaac
    19261 cggcgttggc gtgcaggacg ttctcgaacg tctggtgcgc gacattccgc cgccggaagg
    19321 cgatccggaa ggcccgttgc aggcactaat tatcgactcc tggttcgaca actacctggg
    19381 cgttgtttca cttatccgta ttaaaaacgg caccctgcgt aagggcgaca aagtgaaagt
    19441 catgagtacc gggcagacct ataacgccga ccgtctgggc atcttcacgc cgaaacaggt
    19501 tgaccgcact gaactgaaat gtggcgaagt aggctggctc gtatgtgcga ttaaagatat
    19561 ccacggcgct ccagtcggcg ataccttaac gctggcgcgt aatccggcag aaaaggcgct
    19621 gcctggcttt aagaaagtca aaccgcaggt atacgccggt ctgttcccgg taagttccga
    19681 cgactatgaa gccttccgtg acgcgctggg taaactcagc ctgaacgatg cctcactgtt
    19741 ctatgagccg gaaagctcca gcgcgctggg ctttggtttc cgctgcggct tccttggcct
    19801 gctgcacatg gagatcatcc aggagcgtct ggaacgtgaa tacgatctgg atctgatcac
    19861 cactgcgccg accgtagtgt atgaagttga aaccacgtca agggaagtta tctacgtcga
    19921 cagcccatcc aagctgcctg cggtaaataa catttacgaa ctgcgcgagc cgattgcaga
    19981 gtgtcacatg ctgctgccgc aggcatatct cggcaacgtt attacgctgt gcgtagaaaa
    20041 acgcggcgtg cagaccaata tggtttacca cggtaatcag gtggcgctga cgtacgagat
    20101 cccgatggcg gaagtggtgc tcgacttctt cgatcgcctg aaatctacct cgcgtggtta
    20161 tgcgtctctg gattacaact tcaaacgctt ccaggcgtcc gacatggtac gtgtagacgt
    20221 attaatcaac ggtgaacgtg ttgatgcgct ggcgttgatc acccaccgtg ataattcgca
    20281 aaaccgcggt cgcgagttgg tggagaagat gaaagatctg atcccacgcc agcagtttga
    20341 tatcgccatt caggcagcga ttggtacgca cattattgcg cgatccaccg tgaaacagct
    20401 gcgtaaaaac gtactggcta aatgttatgg cggcgatatc agccgtaaga aaaagctgct
    20461 gcagaagcag aaagaaggta agaaacgcat gaagcagatc ggtaacgttg agctgccgca
    20521 ggaagcgttc ctcgccattc tgcacgtcgg caaagacaac aaataaccct taggagttgg
    20581 catggcgaat atgtttgccc tgattctggt gattgccaca ctggtgacgg gcattttatg
    20641 gtgcgtggat aaattctttt tcgcacctaa acggcgggaa cgtcaggcag cggcgcaggc
    20701 ggctgccggt gactcactgg ataaagcaac gttgaaaaag gttgcaccga agcctggctg
    20761 gctggaaacc ggagcttctg tttttccggt gctggctatc gtattgattg tacgttcgtt
    20821 tatttatgaa ccgttccaga tcccgtcagg ttcgatgatg ccgactctgt taatcggtga
    20881 ttttattctg gtagagaagt ttgcttatgg cattaaagat cctatctacc agaaaacgct
    20941 gatcgaaacc ggtcatccga aacgcggcga tatcgtggtc tttaaatatc cggaagatcc
    21001 aaagcttgat tacatcaagc gcgcggtggg tttaccgggc gataaagtca cttacgatcc
    21061 ggtctcaaaa gagctgacga ttcaaccggg atgcagttcc ggccaggcgt gtgaaaacgc
    21121 gctgccggtc acctactcaa acgtggaacc gagcgatttc gttcagacct tctcacgccg
    21181 taatggtggg gaagcgacca gcggattctt tgaagtgccg aaaaacgaaa ccaaagaaaa
    21241 tggaattcgt ctttccgagc gtaaagagac actgggtgat gtgacgcacc gaattctgac
    21301 agtgccgatt gcgcaggacc aggtggggat gtattaccag cagccagggc aacaactggc
    21361 aacctggatt gttccgccgg gacaatactt catgatgggc gacaaccgcg acaacagcgc
    21421 ggacagccgt tactggggct ttgtgcctga agcgaatctg gtcggtcggg ccacggctat
    21481 ctggatgagc ttcgataagc aagaaggcga atggccgact ggtgtgcgct taagtcgcat
    21541 tggcggcatc cattaatagc catcttcgtt cacgttgtcg ccgttatggc gacaacgtga
    21601 attatttatg agataaatct cccgtggcta acgacatccc ccgtcgttgt gtatagaata
    21661 ttcccccgaa gtttaaggtt ggcaccttca ggttgccacg gcacacgaaa cagcgttggt
    21721 ccccatatac cgctaaactg aaactgcagc gaagcagtta gcagagccat gtatatcagg
    21781 tctgtttcgt gtgctgaatt gttgacgcat ttatttattg gtatcgcatg aaccccatcg
    21841 taattaatcg gcttcaacgg aagctgggct acacttttaa tcatcaggaa ctgttgcagc
    21901 aggcattaac tcatcgtagt gccagcagca aacataacga gcgtttagaa tttttaggcg
    21961 actctattct gagctacgtt atcgccaatg cgctttatca ccgtttccct cgtgtggatg
    22021 aaggcgatat gagccggatg cgcgccacgc tggtccgtgg caatacgctg gcggaactgg
    22081 cgcgcgaatt tgagttaggc gagtgcttac gtttagggcc aggtgaactt aaaagcggtg
    22141 gatttcgtcg tgagtcaatt ctcgccgaca ccgtcgaagc attaattggt ggcgtattcc
    22201 tcgacagtga tattcaaacc gtcgagaaat taatccttaa ctggtatcaa actcgtctgg
    22261 acgaaattag cccaggcgat aaacaaaaag atccgaaaac gcgcttgcaa gaatatttgc
    22321 agggtcgcca tctgccgctg ccgacttatc tggtagtcca ggtacgtggc gaagcgcacg
    22381 atcaggaatt tactatccac tgccaggtca gcggcctgag tgaaccggtg gttggcacag
    22441 gttcaagccg tcgtaaggct gagcaggctg ccgccgaaca ggcgttgaaa aaactggagc
    22501 tggaatgagc atcgataaaa gttactgcgg atttattgcc atcgtcggac gtccgaacgt
    22561 tggcaaatcc acattgttga acaaactgct ggggcagaaa atctccatca cctcccgcaa
    22621 ggcgcagaca actcgtcacc gcattgtggg gatccatact gaaggcgcgt atcaggcgat
    22681 ctacgtcgat acaccgggcc tgcatatgga agaaaaacgc gccattaacc gcctgatgaa
    22741 caaagcggcg agcagctcta ttggcgatgt tgagctggtg atttttgtcg ttgaaggcac
    22801 ccgctggacg ccggacgacg aaatggtgct caacaaactg cgcgaaggca aagcgccggt
    22861 aatcctcgcg gtgaacaaag tggacaacgt gcaggagaaa gccgatctgc tgccgcacct
    22921 gcagttcctg gcaagccaga tgaacttcct cgatatcgtg ccaatctctg ccgaaaccgg
    22981 gctgaatgtt gacactattg cggcaatcgt gcgtaagcat ctgcctgaag cgactcatca
    23041 cttcccggaa gattacatca ccgatcgctc acagcgtttt atggcgtctg aaatcatccg
    23101 cgaaaaactg atgcgtttcc ttggcgctga actgccgtac tccgtgaccg tggagatcga
    23161 acgtttcgtc tctaacgaac gcggcggttg tgacatcaac ggtttgattc tcgttgagcg
    23221 tgaagggcag aagaagatgg ttattggcaa caaaggggcc aagatcaaaa ccatcgggat
    23281 tgaagcgcgt aaagacatgc aggaaatgtt cgaagcgcct gtccaccttg aactgtgggt
    23341 aaaagtgaaa tcgggttggg ccgacgacga acgcgcactg cgcagtctcg gttatgttga
    23401 cgatctttaa gagtcactcc gatggaaggc tggcagcgcg catttgtcct gcatagtcgc
    23461 ccgtggagcg aaaccagcct gatgctggac gtcttcacag aggaatcggg tcgcgtgcgt
    23521 ctggttgcca aaggcgcacg ctctaaacgc tctactctga aaggtgcatt acagcctttc
    23581 acccctctct tgctacgttt tggcgggcgt ggcgaagtca aaacgctgcg cagtgctgaa
    23641 gctgtctcac tggcgctgcc attaagcggt atcacgcttt acagcggtct gtacatcaac
    23701 gaacttctct cccgcgtact ggaatacgag acgcgcttct ctgaactctt tttcgattac
    23761 ttgcactgca ttcagtctct tgcaggtgac actggtacgc cagaacccgc gctgcgtcgc
    23821 tttgaactgg cactactcgg gcatctgggc tatggcgtca attttaccca ttgcgcgggt
    23881 agcggcgagc cggtagatgg caccatgacg tatcgttatc gcgaagaaaa agggtttatc
    23941 gcaagcgtcg ttatcgacaa taaaacgttc accggaaggc agttaaaagc gttaaacgcg
    24001 cgggaatttc ctgacgcaga cacactacgc gccgcgaaac gctttacccg aatggcgctt
    24061 aagccgtatc ttggcggtaa accattaaag agcagggaac tgttccggca gtttatgcct
    24121 aagcgaacgg tgaaaacaca ttatgaatga tgaggattgt catggctgaa ttactgttag
    24181 gcgtcaacat tgaccatatt gcaacgctgc gtaacgcacg cggtactgct tacccggatc
    24241 cggtgcaggc ggcgtttatt gccgagcagg cgggagcgga cggcattacc gtgcatttac
    24301 gtgaagatcg ccgccacatt actgaccgcg atgtgcgcat cctgcggcag acgctggata
    24361 cccgaatgaa tctggagatg gcagtgaccg aagagatgct ggcgatcgcc gttgagacga
    24421 agccacattt ttgctgcttg gtaccggaaa agcgtcagga agtaacaacc gaaggcggcc
    24481 tggacgttgc agggcagcgt gaaaaaatac gcgatgcctg caaacgtctg gcagatgccg
    24541 ggattcaggt ttctctgttt atcgacgccg atgaagagca gatcaaagct gcggcagagg
    24601 ttggcgcacc gtttatcgag atccacaccg gttgctatgc tgatgccaaa actgacgccg
    24661 aacaggcgca agagctggtg cgtatcgcca aagccgcgac ctttgccgct agtctcggtc
    24721 tgaaagttaa cgccggacac ggtctgacct atcacaacgt gaaagccatt gccgccatcc
    24781 ctgagatgca tgaactgaat atcggtcatg ccattattgg ccgtgcagtg atgaccggac
    24841 tgaaagatgc ggtggcagaa atgaagcgtc tgatgctgga agcgcgtggc taatggcaat
    24901 attaggttta ggcacggata tcgtagagat cgctcgcata gaagcagtga tcgcccgatc
    24961 cggtgagcgc ctggcacgcc gcgtattaag cgataacgaa tgggcaatct ggaaaacgca
    25021 ccaccagccg gtgcgttttc tggcgaagcg ttttgctgtg aaagaagccg cagcaaaagc
    25081 gtttggcacc gggatccgca atggtctggc gtttaatcaa tttgaagtat tcaatgatga
    25141 gctcggcaaa ccacggctac ggctatgggg tgaggcatta aaactggcgg aaaagctcgg
    25201 cgttgtaaat atgcatgtaa cgctggcaga tgaacgccac tatgcttgtg ccacggtaat
    25261 tattgaaagt taaaataata ccgttctctt taaaaagtaa aacccccgaa ctggtaagga
    25321 acgggggttt tactttttaa ctttaatttt gtgcagcatg caccctcaac aacagcgatt
    25381 atggatcagt gtacttatct tttacaagca acttgcaatc tttagcataa aaactcgagc
    25441 ctttacgaag aaagcaatat tgatggaaag attaacgtga ccgccaattc gtaagtacat
    25501 taaaattggc ttcgttattg aagattttgc tgtgctttac accatgccac agaattcccc
    25561 cattgaaacg agtggtgtcg tcaaagctct ggtgtggagt gcagcatgca ccctcaataa
    25621 ctcgcacgtt cagttttggg gagatgtaag ggctaatctg aatggctgca ttccttgttt
    25681 aaggaaaaac gaatgactga ttgccgatac ctgattaaac gggtcatcaa aatcatcatt
    25741 gctgttttac agctgatcct tctgttctta taacacaagg aaacgtactt aaggtgcgtc
    25801 cggtgaacca atcggacgcg cctttaataa ctataaataa gtgtcttggc agatactata
    25861 taaattaact tagtgaatga ttatgctaat gtcatcaatt aaataaatat aatggcgtta
    25921 aggcttccca gtaatataat taatactcta cttccagagt agaatattaa attttatccg
    25981 cgtggtgcat cagcacaaat ttatcccaca actgttcttc tgtctcgaca tgcgccggat
    26041 ctttcacaat agtattgggg atcgggcaca ccttctggca ggttggtgtc tcgtagtgcc
    26101 ctacgcattc ggtacactta tcgctgttaa tttcgtagat atgatctccc attgaaatcg
    26161 cctcattcgg gcattcgggt tcacacatat cacaattaat acagcgttta gtaattagta
    26221 aagacatttc aatggattac cgttaaatca ttttaaaatc agtaagttgt atcgagtttg
    26281 tatgctttac tgtcattaac ttactgtatg ttgatccagt gtatttaacc ttgataaact
    26341 cagtccagca acacaaaacc gcaacacatt gcattttgtc ccgtagaaaa gacttgtatg
    26401 tgtgagcttg ttttctgcgc ctacgcagat aaggattgag aatgccgcgc actgtaacac
    26461 ataatccgga tagccccaat aatgacgatg ttttagccgc atctgaaaaa tgggacgcct
    26521 gtaaaccccc ctataccagc gcacacatga aaatctgtgt tgctgccgcc aaaatcatcc
    26581 tcgctgcttc cggcgtggct cgctgttcca aatacgaaaa agagaactat ctccgtatcg
    26641 atttcagcaa agccggtaag gttacatttt acgccgagtt tccaaaaaag atgggcctca
    26701 aaggtaaaaa gctcggcgag tggccggagc tcgttatcca gctggcgcgc gaaaaagcac
    26761 taggtatggc tgacggtgga ctgcgggcag agtccgtaca tgcagcgctg gaaatgtacc
    26821 gggatgaccc aaagccaaag tagcccggca gaagctgagc ccggacagtt tcacaaccta
    26881 cggggtgcgt atcgaccgga ttaaagtaac gttcggcgag cgtgaggtgt tcagcgacgt
    26941 aacatacaat cggctggtgg aagtgctgga cgagtggatc gccactcgct cgaacaataa
    27001 cgccctggag ttgtttgccg agctccgtcg gttctggaag ttctgcgcac ctactctttg
    27061 caacggccgc aatgttgccg ccagtctgcc agatgattat gtttcctccc gcgtacagaa
    27121 acctaccccc acacggcttt ttaccgatat tgaatcaatc gcccgactct ggctcaatgt
    27181 tgctgcctgc acctctgtac accagaagaa tgctgttcgc ttcatgatca tcactggtgt
    27241 tcgtccgatt aatgtccata acctgcgctg ggactacgtt tacgaggagg ctggtgaaat
    27301 tgtttatccg gaaggggtta tcggcatgcg aggggctatg aaaacacaaa aggctttccg
    27361 cctgccgata acgcctgaga tccggcggat tatcgacgag cagaaagcct ggcgtgattc
    27421 agttcctgag tgcaacaggg attatgtatt tttgcagcca cgtgatccaa tgcagccatt
    27481 ttcaaaacga tcactggata agctggtgaa aacatacagc ccggacgggg ctgtaaaatg
    27541 aggtgtactg gcaatagcgg acactaccat ttgttctttt tttaagcagc catctgatga
    27601 t
//
LOCUS       DAEBXN010000051        27371 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.051, whole genome shotgun sequence.
ACCESSION   DAEBXN010000051 DAEBXN010000000
VERSION     DAEBXN010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27371
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.051"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            131..574
                     /locus_tag="KJL99_002215"
     CDS             131..574
                     /locus_tag="KJL99_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004997737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBD4436933.1"
                     /translation="MDLALVTGQRKGDLAAMRWEDIRDSRLYVEQQKTGAKIRISLPT
                     TISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFH
                     EIRSLSGRLHSAEKGSDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            810..1628
                     /gene="ybhA"
                     /locus_tag="KJL99_002216"
     CDS             810..1628
                     /gene="ybhA"
                     /locus_tag="KJL99_002216"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HBD4436934.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETTQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIAHGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(1629..2687)
                     /gene="modC"
                     /locus_tag="KJL99_002217"
     CDS             complement(1629..2687)
                     /gene="modC"
                     /locus_tag="KJL99_002217"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HBD4436935.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLGEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2690..3379)
                     /gene="modB"
                     /locus_tag="KJL99_002218"
     CDS             complement(2690..3379)
                     /gene="modB"
                     /locus_tag="KJL99_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HBD4436936.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPSETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3379..4152)
                     /gene="modA"
                     /locus_tag="KJL99_002219"
     CDS             complement(3379..4152)
                     /gene="modA"
                     /locus_tag="KJL99_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBD4436937.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4319..4468)
                     /gene="acrZ"
                     /locus_tag="KJL99_002220"
     CDS             complement(4319..4468)
                     /gene="acrZ"
                     /locus_tag="KJL99_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HBD4436938.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            4597..5385
                     /gene="modE"
                     /locus_tag="KJL99_002221"
     CDS             4597..5385
                     /gene="modE"
                     /locus_tag="KJL99_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HBD4436939.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            5453..6925
                     /gene="modF"
                     /locus_tag="KJL99_002222"
     CDS             5453..6925
                     /gene="modF"
                     /locus_tag="KJL99_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HBD4436940.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPSEDYTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDKIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANESRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSSETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQVVSDRQQKLVQQWLDILGIDK
                     HTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            7186..8202
                     /gene="galE"
                     /locus_tag="KJL99_002223"
     CDS             7186..8202
                     /gene="galE"
                     /locus_tag="KJL99_002223"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HBD4436941.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVAESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPQIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            8212..9258
                     /gene="galT"
                     /locus_tag="KJL99_002224"
     CDS             8212..9258
                     /gene="galT"
                     /locus_tag="KJL99_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HBD4436942.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAECEDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            9262..10410
                     /gene="galK"
                     /locus_tag="KJL99_002225"
     CDS             9262..10410
                     /gene="galK"
                     /locus_tag="KJL99_002225"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HBD4436943.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            10404..11444
                     /gene="galM"
                     /locus_tag="KJL99_002226"
     CDS             10404..11444
                     /gene="galM"
                     /locus_tag="KJL99_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HBD4436944.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            11647..12399
                     /gene="gpmA"
                     /locus_tag="KJL99_002227"
     CDS             11647..12399
                     /gene="gpmA"
                     /locus_tag="KJL99_002227"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HBD4436945.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(12566..13618)
                     /gene="aroG"
                     /locus_tag="KJL99_002228"
     CDS             complement(12566..13618)
                     /gene="aroG"
                     /locus_tag="KJL99_002228"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HBD4436946.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLVNAVKARRG"
     gene            13934..14314
                     /locus_tag="KJL99_002229"
     CDS             13934..14314
                     /locus_tag="KJL99_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HBD4436947.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14428..15369
                     /gene="zitB"
                     /locus_tag="KJL99_002230"
     CDS             14428..15369
                     /gene="zitB"
                     /locus_tag="KJL99_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HBD4436948.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHTTIQMEYQPCHRPDCHLNEGVSGHSHHHH"
     gene            complement(15366..16085)
                     /gene="pnuC"
                     /locus_tag="KJL99_002231"
     CDS             complement(15366..16085)
                     /gene="pnuC"
                     /locus_tag="KJL99_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HBD4436949.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(16123..17166)
                     /gene="nadA"
                     /locus_tag="KJL99_002232"
     CDS             complement(16123..17166)
                     /gene="nadA"
                     /locus_tag="KJL99_002232"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HBD4436950.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQVECSLDLGCPVEEFNAFYDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDAFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNDLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(17415..17490)
                     /locus_tag="KJL99_002233"
     tRNA            complement(17415..17490)
                     /locus_tag="KJL99_002233"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17455..17457),aa:Lys,seq:ttt)
     gene            complement(17494..17569)
                     /locus_tag="KJL99_002234"
     tRNA            complement(17494..17569)
                     /locus_tag="KJL99_002234"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17534..17536),aa:Val,seq:tac)
     gene            complement(17621..17696)
                     /locus_tag="KJL99_002235"
     tRNA            complement(17621..17696)
                     /locus_tag="KJL99_002235"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17661..17663),aa:Lys,seq:ttt)
     gene            complement(17699..17774)
                     /locus_tag="KJL99_002236"
     tRNA            complement(17699..17774)
                     /locus_tag="KJL99_002236"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17739..17741),aa:Val,seq:tac)
     gene            complement(17910..17985)
                     /locus_tag="KJL99_002237"
     tRNA            complement(17910..17985)
                     /locus_tag="KJL99_002237"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17950..17952),aa:Lys,seq:ttt)
     gene            complement(18150..18941)
                     /gene="cpoB"
                     /locus_tag="KJL99_002238"
     CDS             complement(18150..18941)
                     /gene="cpoB"
                     /locus_tag="KJL99_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HBD4436951.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVSSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGATASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(18951..19472)
                     /gene="pal"
                     /locus_tag="KJL99_002239"
     CDS             complement(18951..19472)
                     /gene="pal"
                     /locus_tag="KJL99_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HBD4436952.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(19507..20799)
                     /gene="tolB"
                     /locus_tag="KJL99_002240"
     CDS             complement(19507..20799)
                     /gene="tolB"
                     /locus_tag="KJL99_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HBD4436953.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(20932..22173)
                     /gene="tolA"
                     /locus_tag="KJL99_002241"
     CDS             complement(20932..22173)
                     /gene="tolA"
                     /locus_tag="KJL99_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HBD4436954.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEVAAAKAAADAKAAEEAAKKAAADAK
                     KKAEAEAAKAAAEAQKKAEVAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKK
                     AAAEKAAADKKAAAEKAAADKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEK
                     AAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAG
                     QIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKI
                     PKPPSQAVYEVFKNAPLDFKP"
     gene            complement(22238..22666)
                     /gene="tolR"
                     /locus_tag="KJL99_002242"
     CDS             complement(22238..22666)
                     /gene="tolR"
                     /locus_tag="KJL99_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HBD4436955.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(22670..23362)
                     /gene="tolQ"
                     /locus_tag="KJL99_002243"
     CDS             complement(22670..23362)
                     /gene="tolQ"
                     /locus_tag="KJL99_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HBD4436956.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(23359..23763)
                     /gene="ybgC"
                     /locus_tag="KJL99_002244"
     CDS             complement(23359..23763)
                     /gene="ybgC"
                     /locus_tag="KJL99_002244"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HBD4436957.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(23913..24206)
                     /gene="ybgE"
                     /locus_tag="KJL99_002245"
     CDS             complement(23913..24206)
                     /gene="ybgE"
                     /locus_tag="KJL99_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HBD4436958.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(24206..24319)
                     /gene="cydX"
                     /locus_tag="KJL99_002246"
     CDS             complement(24206..24319)
                     /gene="cydX"
                     /locus_tag="KJL99_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HBD4436959.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(24334..25473)
                     /gene="cydB"
                     /locus_tag="KJL99_002247"
     CDS             complement(24334..25473)
                     /gene="cydB"
                     /locus_tag="KJL99_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HBD4436960.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLL
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(25489..27057)
                     /gene="cydA"
                     /locus_tag="KJL99_002248"
     CDS             complement(25489..27057)
                     /gene="cydA"
                     /locus_tag="KJL99_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HBD4436961.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQTVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
BASE COUNT         6888 a         7256 c         6706 g         6521 t
ORIGIN      
        1 tgccgaagaa gatccagctt ctgcaaccag gccattcaga actgaagtga agcgcagtcg
       61 actgttaatt gacgagtatc tgttaatcag gaaaatagca gacgtccaga acgaatggtt
      121 cggcctttgc atggatcttg cactggtaac aggacagcgc aaaggagatc tggcggctat
      181 gagatgggag gatataagag atagcaggct atacgtggaa caacaaaaaa caggggcaaa
      241 aattcgcatt tctctcccca ccacgatttc cagattgaat ttaacacttg ccgatgtact
      301 ggataacttg aaaaaaataa acggtaaaaa cgaaaagtta ctcggaggga agacagccag
      361 gacaattgca gcacagttca ggattgcccg agacacatcc ggattgaaat gggaaggtga
      421 cccaccgcca tttcatgaaa tccgaagttt atccggtcgc ctgcactcag cagaaaaagg
      481 cagtgatttc actcaggcgc tccttggaca tcgttcatcc agcatgacgg ataaataccg
      541 tgacggacga gggagggaat ggaaagacat ttagaattaa tttcgaatga tttcgaaatg
      601 ccacttgtaa ttaattgatt ataaagaatt aattcctaaa gagcatttgc ttcatgaatg
      661 ctcctttgca tttagcaact gactgtaaca gctaaaatta gtcgcttttg gcggtgaatg
      721 ctgaattaaa acgcagaatt ttgtccgata gtggtatcgg tgtaccattc gcgcagacga
      781 aaattcttaa ccttaatctg gaatacgcca tgaccacacg cgtgattgct ctcgacttag
      841 acggcacctt attgaccccg aaaaagacct tgcttccatc atcgatagaa gccctggccc
      901 gcgctcgcga agcaggctat caattaatca tcgtcacagg tcgccatcac gtcgctattc
      961 atccttttta tcaggcgctg gcgctggata cacctgctat ttgctgtaat ggcacctatt
     1021 tgtatgatta tcatgcaaaa accgtgctgg aagcggaccc aatgcccgtt aataaagccc
     1081 tgcaactcat tgagatgctg aatgaacacc acattcacgg tctgatgtat gtcgatgatg
     1141 caatggtcta tgagcacccg accgggcatg tcattcgcac gtctaactgg gcgcaaaccc
     1201 tgccgccgga acagcgtccg actttcacac aagtcgcttc tctggctgaa acgacgcaac
     1261 aagttaacgc cgtatggaag ttcgccctga cgcacgatga cctgccgcaa ttgcagcatt
     1321 ttggtaagca tgtcgaacat gaactgggac tggagtgtga atggtcctgg cacgatcagg
     1381 ttgatattgc acacggcggc aacagcaaag gtaaacgttt gacgaaatgg gttgaggcgc
     1441 aaggttggtc gatggaaaac gtcgtggcat tcggcgataa ctttaatgat atcagtatgc
     1501 tggaagccgc tggtacaggc gtggcgatgg gcaacgccga tgacgcggta aaagcgcgcg
     1561 ccaacattgt gattggtgat aacaccaccg acagcattgc ccagttcatt tatagccacc
     1621 tgatttaatc aggcggttat cgacacactt ttaatttgcg cgtacagcca caggccaggt
     1681 ttgatcgcca gttcatccct ggcccacggg ctgatacgcg cccacagcgt tttaccgccg
     1741 acttccagct ccacttccac ctggccgttg tcgtcataac tattaacaac ttttgcccgc
     1801 aatacgttac gaatgctggt ttgctgcggc ggttgtaaaa ccagagaaac atcggaagcc
     1861 tgaatgcgga tacgtagcgc agcttgcagc ggttcgccca gcttattgac ccacaaatgc
     1921 tgatcgccca gcgccagcgc ggtcatcgcg taatgcggat gatgctcaag caccgtcact
     1981 ttcagaatgc tactttgttg ctctttcggc agccacggat tcatcacgct actgccccac
     2041 acttcctcca gcgcgccaaa ggctttcacc tgaccgtttt ccagtaccat cactctgtct
     2101 gccagatgga ggatctcatc cagcgaatgg ctgacataca acatcggaat gttgatttcc
     2161 cgtgtcagcc gttgcagata aggcaacagt tcgcgtttac gcggaatatc cagtgacgcc
     2221 agcggttcat ccagcaacag caattccggt gctgtcagca aagcccgacc aatcgccacg
     2281 cgctgttttt cgcctccgga caggctgcct ggtaaacggt caagcaacgg ttcaatgcct
     2341 aaaagcgcca ccagcttatc gaactgatcg accatacttt tcgacatgcc gtagcgcaga
     2401 ttgccacgca ctttgtaatg cgggaacaac cgcgcatcct gaaaaacata gccaacacga
     2461 cgcttttccg gcgtcaggca gatacctttt tcggcatcat ttagtacccg cccattgagg
     2521 acaatccgcc ctttttgcgg gcgcgtcagt ccactgatgg cgttaatcag cgaagttttt
     2581 ccggcaccgg agacgccaaa gatagcagtg atgccattgg cgggcagcgt ttcattaatg
     2641 gtcaggcaat ggttgcccaa cgtctgggaa aaattcagct ccagcatgat tagcgccccg
     2701 cccgttcacg gctgattctg gccagccatt ctgaaatcaa cagggagatc atcgccagcg
     2761 caatagaaat aatgcacagt ctcgccgcgc cactttcccc gccgggggtc tggatcaggg
     2821 tatacatggc agaaggaatg gttcgcgttt cgctaggaat gttcgacaca aaggtgatgg
     2881 ttgcaccaaa ctcaccgaga gaacgagcaa aagccagtac cgtaccaaca ataattcccg
     2941 gtaaggtcag cggtaacgtg atagtaaaga aaacgcgcca gcgcccggca cctagtgttc
     3001 ttgcggcctg ttccagtttg acatcaaccc cttccagcgc cagacgaatt gcccgcacca
     3061 tcagcggaaa cgacatgacg gcggcagcga gaaccgcgcc gcgccagcta aaggcgaagg
     3121 taataccaaa ccagtcatac agacgttcac cgataaatcc gcgccgtccc atcgaaacta
     3181 ataataagta accgacgacc acgggcggta acaccagcgg tagatgcagt acgctgtcga
     3241 gcagagcttt gcccggaaac gtgcaacgca ccagtaacca ggcaaaaaag atcccaaacg
     3301 gcaggctaaa cagcacagcc agggaagaaa ctttcaggct taataaaact gcctgccatt
     3361 ctggatcggt cagtatcatt acttggttgt aaatccgtaa cgtttaaaga tttccgctgc
     3421 ctgcggtccc ttcagataat cataaaatgc tttcactgtc gcattgttat gcccttccac
     3481 aacagcaacc ggatattcca cttttttatg tgaatcttcc gggaaggtgg caaccacttt
     3541 tacccctttg ctggcaactg cgtcagaacc gtagacaatg cccagaggcg cttcgttacg
     3601 ttcgaccagc gccagcgccc cacgaacatc ttccgctggg gccagtttcg gagagagcgt
     3661 atcccatgcg cccagttttt gcagtgcttc ttttgcataa atgccagcgg gaacatgttc
     3721 cggatcgcca accgccaggc gaccgccatt cagcagcgaa gtccagttag ttttgctgtc
     3781 gatggtgaaa tctttctgct cgctggcttt cggtgctaca acgaccaggc tattgccgag
     3841 cagtgtctga cgcgtagcag tatcgatcgc ttttttatca accgcataat ccatccattt
     3901 ctgatcggca gaaataaaca gatccgcagg ggcacccgct tcaatctgac gggcgagagt
     3961 agatgacgaa gcgaaagaag aaaccacatc cacgcctttc tcttttttat actgcgtagc
     4021 aatgtcctgc attgcgttag tcagtgatgc ggcggcgaac accgtgattt tcccttcatc
     4081 tgccagtgca ttgccagcaa ctgcgaaaga gagtgccgcc ccggcaaaca agttcaacca
     4141 tttacgagcc attggtaacc ccttaatgta acgttatgta ggcgacaata taacgactaa
     4201 ctcggaattt tcccagcagt tattgctaac ctttgtgagg tagataagaa aaaatatcgg
     4261 caggaaaagc aggaagttga gaaaaagaaa atgcccgact aagcgggcac tcagggaatc
     4321 aatgattttg tccgggctgg tcttttttac caacaccaga aaagatgttg aatacttcac
     4381 caagaccgta aatcagaccc aggatgatgg ccatcacgac aggtaccatg attacggcga
     4441 ataccagact ttttaataac tctaacatgg tcaactccag atatagtcat gagactattc
     4501 taaccgctaa gcacagaaaa gcactcccct tttgtgcggt cagctttgcg tggcgttcgt
     4561 tttccgtcac aataagactt ttgccaggac attgttatgc aggccgaaat ccttctcacc
     4621 cttaagctcc aacaaaaatt attcgccgac ccgcgccgca tttcgctact aaaacacatt
     4681 gcgctttccg gttccattag ccagggagcg aaagatgccg gtatcagcta taaaagcgcc
     4741 tgggatgcca ttaacgagat gaatcagtta agtgagcata ttctggtcga gcgcgcaaca
     4801 ggcggtaaag gtggcggagg cgcagtactg acccgctatg gtcagcgact gattcagctc
     4861 tatgacttac tggcgcaaat ccagcaaaaa gcctttgatg tgttaagtga cgatgacgcc
     4921 ctgccgctga acagcctgct ggccgcgatc tcacgttttt cactgcaaac cagcgcccgt
     4981 aaccagtggt tcggtaccat caccgcccgc gatcatgatg acgttcaaca gcatgttgat
     5041 gtcttactgg ctgacggaaa aacacgcctg aaagtcgcaa ttaccgcaca aagcggcgca
     5101 cgtctggggc tggatgaagg caaagaagtg ttgatattgc taaaagcgcc gtgggtaggc
     5161 attactcagg acgaagcggt cgcacaaaac gccgacaacc aattaccggg tattattagt
     5221 catattgagc gcggcgcaga gcagtgcgaa gtattaatgg cgctacccga cgggcaaaca
     5281 ctgtgcgcca cagtgccggt aaatgaagcg acttctcttc agcaagggca gaatgtcacg
     5341 gcctacttta atgccgacag cgtgattatc gccacgctgt gctaagcgtg ttgacaattt
     5401 gttatgaaac acgtatccct gtcagtaatc gctgcacaaa atgggatata aaatgtcatc
     5461 gttgcaaatt ttgcaaggca cgtttcgtct tagcgacaca aaaacgctgc aattgcctca
     5521 gctaacgtta aacgcgggtg atagttgggc gtttgtcggt tcgaatggaa gtggaaagtc
     5581 ggcgctggcc cgcgcgctgg cgggggaact tccgcttttg aaaggtgaac ggcaaagcca
     5641 gttttcccac atcactcgtc tctccttcga gcaattgcaa aagctcgtca gcgacgaatg
     5701 gcaacggaat aacaccgata tgctcggccc tagcgaagat tacaccggac gcactacggc
     5761 tgagattatt caggatgaag taaaggatgc accgcgttgc atgcaactgg cgcagcagtt
     5821 cggtattacc gccctcctcg accgacgctt taaatacctt tccactggcg agacgcgaaa
     5881 aaccctgctg tgtcaggcgc tgatgtcgga gcctgacttg ttgattcttg atgagccgtt
     5941 cgatggcctg gatgttgcct cacgtcagca gctggctgag cgactcgcct cgttacatca
     6001 gtccggtatt actttggtac tggtgctcaa tcgcttcgat aagatcccgg aatttgtcca
     6061 gtttgctggc gtgctggcgg attgtacgtt agcggaaact ggcgctaaag aggaactgct
     6121 ccagcaagca ctcgtcgcgc aacttgcgca tagtgaacag cttgaaggtg tgcaactgcc
     6181 ggagccggat gaaccttcag cacgtcacgc cttacccgcc aacgaatcgc gcattgtgct
     6241 gaacaatggc gtggtttctt ataatgatcg ccccattctt aataacctta gctggcaggt
     6301 gaatccaggc gaacactggc aaattgtcgg gccaaatggc gcgggaaaat cgacgttatt
     6361 aagcctgatt actggcgatc atccgcaagg ttacagcaac gatttgacgc ttttcggccg
     6421 acgtcgcggc agcagcgaaa ccatctggga catcaaaaag catatcggtt acgtcagcag
     6481 tagtttgcat ctggattacc gggtcagcac taccgtgcgt aatgtgattc tttctggcta
     6541 ttttgattcg attggcattt atcaggtcgt ttcggatcgc cagcaaaaac tggtgcagca
     6601 gtggctggat attctcggca ttgataaaca cacggctgac gctccgttcc atagtctttc
     6661 ctggggacag cagcgtttgg cgctgatcgt ccgcgcactg gtgaaacatc cgacgttgct
     6721 tattctcgat gaaccactac aggggcttga tccgctcaat cgccaactta tccgccgttt
     6781 tgttgatgtg ctgattagcg aaggtgaaac gcaattgttg tttgtttcgc accacgctga
     6841 agatgcgcct gcctgtatta cccatcgcct tgagttcgtg ccggacggtg gactctatcg
     6901 ctatgtgctg acaaaaatat attgagtcgg tagtgctgac cttgccggag gcggcctaag
     6961 cgcactctcc ggccaacggt tcgacgcatg caggcatgaa accgcttctt ttttcagata
     7021 aaaagcgcaa tcattcataa accctctgtt ttataatcac ttaatcgcgc ataaaaaacg
     7081 gctaaattct tgtgtaaacg attccactaa tttattccat gtcacacttt tcgcatcttt
     7141 gttatgctat ggttatttca taccataagc ctaatggagc gaattatgag agttctggtt
     7201 accggtggta gcggttacat tggaagtcat acctgcgtgc aattactgca aaacggtcat
     7261 gatgtcatca ttcttgataa cctctgtaac agtaagcgca gcgtactgcc tgttatcgag
     7321 cgtttaggcg gcaaacatcc aacgtttgtt gaaggcgata ttcgtaacga agcgttgatg
     7381 accgagatcc tgcacgatca cgctatcgac accgtgatcc acttcgccgg gctgaaagcc
     7441 gtcgccgaat cggtacaaaa accgctggaa tattacgaca acaatgtcaa cggcactctg
     7501 cgcctgatta gcgccatgcg cgccgctaac gtcaaaaact ttatttttag ctcctccgcc
     7561 accgtttatg gcgatcagcc ccaaattcca tacgttgaaa gcttcccgac cggcacaccg
     7621 caaagccctt atggcaaaag caaattgatg gtggaacaga tcctcaccga cctgcaaaaa
     7681 gcccagccgg actggagcat tgccctgctg cgctacttca acccggttgg cgcgcatccg
     7741 tcgggcgata tgggcgaaga tccgcaaggc attccgaata acctgatgcc atacatcgcc
     7801 caagttgctg taggccgtcg cgactcgctg gcgatttttg gtaacgatta tccgaccgaa
     7861 gatggtactg gcgtacgcga ttacatccac gtaatggatc tggcggacgg tcacgtcgtg
     7921 gcgatggaaa aactggcgaa caagccaggc gtacacatct acaacctcgg tgctggcgta
     7981 ggcagcagcg tgctggacgt ggttaatgcc ttcagcaaag cctgcggcaa accggttaac
     8041 tatcattttg caccgcgtcg cgaaggcgac cttccggcct actgggcgga cgccagcaaa
     8101 gccgaccgtg aactgaactg gcgcgtaacg cgcacactcg atgaaatggc gcaggacacc
     8161 tggcactggc agtcacgcca tccacaggga tatcccgatt aaggaacgac catgacgcaa
     8221 tttaatcccg ttgatcatcc acatcgccgc tacaacccgc tcaccgggca atggattctg
     8281 gtttcaccgc accgcgctaa gcgcccctgg cagggggcgc aggaaacgcc agccaaacag
     8341 gtgttacctg cgcacgatcc agattgcttc ctctgcgcag gtaatgtgcg ggtgacaggc
     8401 gataaaaacc ccgattacac cgggacttac gttttcacta atgactttgc ggccttgatg
     8461 tctgacacgc cagatgcgcc agaaagcaac gatcctttga tgcgttgcca gagcgcgcgc
     8521 ggtaccagcc gggtgatctg cttttcaccg gatcacagta aaacgttgcc agaactgagc
     8581 gttgcggcat tgacggaaat cgtcaaaacc tggcaggagc aaaccgcaga gctgggaaaa
     8641 acgtacccgt gggtgcaggt ctttgaaaac aaaggtgcgg cgatgggctg ctctaacccg
     8701 catccgcacg gacagatttg ggcaaatagc ttcctgccta acgaagctga gtgcgaagac
     8761 cgcctgcaaa aagaatattt tgccgaacag aaatcaccaa tgctggtgga ttatgttcag
     8821 cgcgagctgg cagacggtag ccgtaccgtt gtcgaaaccg aacactggtt agccgttgtg
     8881 ccttactggg ctgcctggcc gttcgaaacg ctactgctgc ccaaagccca cgttttgcgg
     8941 atcaccgatt tgaccgacgc ccagcgcagc gatttggcgc tggcgttgaa aaagctgacc
     9001 agtcgttatg acaacctctt ccagtgctcc ttcccctact ctatgggctg gcacggcgca
     9061 ccatttaatg gcgaagagaa tcaacactgg cagctgcacg cgcactttta tccgcctctg
     9121 ctgcgctccg ccaccgtacg taaatttatg gttggttatg aaatgctggc agagacccag
     9181 cgagacctga ccgcagaaca ggcagcagag cgtttgcgcg cagtcagcga tatccatttt
     9241 cgcgaatccg gagtgtaaga aatgagtctg aaagaaaaaa cacaatctct gtttgccaac
     9301 gcatttggct accctgccac tcacaccatt caggcgcctg gccgcgtgaa tttgattggt
     9361 gaacacaccg actacaacga cggtttcgtt ctgccctgcg cgattgatta tcaaaccgtg
     9421 atcagctgtg caccacgcga tgaccgtaaa gttcgcgtga tggcagccga ttatgaaaat
     9481 cagctcgacg agttttccct caatgcgccc attgtcgcgc atgaaaacta tcaatgggcg
     9541 aactacgttc gtggcgtggt gaaacatctg caactgcgta acaacagctt cggcggtgtg
     9601 gacatggtga tcagcggcaa tgtgccgcag ggtgccgggt taagttcttc cgcttcactg
     9661 gaagtcgcgg tcggaaccgt attgcagcag ctttatcatc tgccgctgga cggcgcacaa
     9721 atcgcgctta acggtcagga agcagaaaac cagtttgttg gctgtaactg cgggatcatg
     9781 gatcagctaa tttccgcact cggcaagaaa gatcatgcct tgctgattga ctgccgctca
     9841 ctggggacta aagcagtttc catgccgaaa ggtgtggctg tcgtcatcat caacagtaac
     9901 ttcaaacgta ccctggttgg cagcgaatac aacacccgtc gtgaacagtg cgaaaccggt
     9961 gcgcgtttct tccagcagcc agctctgcgc gatgtcacca ttgaagagtt caacgctgtt
    10021 gcacatgagc tggacccaat cgtggcgaaa cgcgtgcgtc atatcctgac tgaaaacgcc
    10081 cgcaccgttg aagctgccag cgcgctggag caaggcgacc tgaaacgtat gggcgagttg
    10141 atggccgagt ctcatgcctc tatgcgcgat gatttcgaaa tcaccgtgcc gcaaattgac
    10201 actctggtag aaatcgtcaa agctgtgatt ggcgacaaag gtggcgtacg catgaccggc
    10261 ggcggatttg gcggctgtat cgtcgcgctg atcccggaag agctggtgcc tgccgtacag
    10321 caagctgtcg ctgaacaata tgaagcaaaa acaggtatta aagagacttt ttacgtttgt
    10381 aaaccatcac aaggagcagg acagtgctga acgaaactcc cgcactggca cccgatggtc
    10441 agccgtaccg actgttaact ttgcgtaaca acgcagggat ggtagtcacg ctgatggact
    10501 ggggtgcgac tttactttcc gcccgtattc cgctttccga tggcagcgtc cgcgaggcgc
    10561 tgctcggctg tgccagtccg gaatgctatc aggatcaggc tgcgtttctg ggggcctcta
    10621 ttggtcgtta tgccaaccgc atcgccaata gtcgctatac ctttgacggt gaaaccgtga
    10681 cgctttcgcc aagccagggc gttaaccagc tgcacggcgg cccggaaggg ttcgacaaac
    10741 gtcgctggca gattgtgaac cagaacgatc gtcaggtgct gtttgccctg agttcagatg
    10801 atggcgatca gggcttcccg ggtaatctcg gcgcgacggt gcaatatcgt ctgaccgacg
    10861 ataaccgtat ctccattact tatcgcgcca cagttgataa accttgcccg gtgaatatga
    10921 ctaatcacgt ctatttcaat cttgacggcg agcagtctga cgtgcgcaat cacaagttgc
    10981 agattctggc ggacgaatat cttccggttg atgaaggcgg cattccgcac gacggcttga
    11041 aatctgtcgc cggaacgtct tttgatttcc gcagcgccaa aatcatcgcc agtgagtttc
    11101 ttgccgacga cgatcagcgc aaagtgaaag gttacgatca cgcattcttg ttacaggcca
    11161 aaggcgatgg caagaaagtg gcggcgcatg tctggtcagc agatgaaaaa ttgcagctga
    11221 aggtctacac caccgctccg gctcttcaat tctactccgg caacttcctc ggcggcacac
    11281 cgtcgcgggg aaccgaacct tacgccgact ggcaagggct ggcgttggaa agcgagtttc
    11341 tgccggacag cccgaaccac cctgaatggc cgcaaccgga ctgcttcctg cgtcctggcg
    11401 aagagtattc cagcctgacg gaatatcagt ttattgctga gtaatgttaa gccctcgtca
    11461 tgagggcttt atcttcatat tgttcaaatc accagcaaac accgacatat ttgcaactca
    11521 atattcacaa caaccttaca ctgcgccact attttcgcta tggttatgcg taagcattgc
    11581 tgttgcttcg tcacggcaat ataatgagaa ttattatcat taaaagatga tttgaggagt
    11641 aagcatatgg ctgtaactaa gctggttctg gttcgtcatg gcgaaagtca gtggaacaaa
    11701 gaaaaccgtt tcaccggttg gtacgacgtg gatctgtctg agaaaggcgt aagcgaagca
    11761 aaagcagcag gtaagctgct gaaagaggaa ggttacagct ttgactttgc ttacacttct
    11821 gtgctgaaac gcgctatcca taccctgtgg aatgtgctgg acgaactgga tcaggcatgg
    11881 ctgcccgttg agaaatcctg gaaactgaac gaacgtcact acggtgcgtt gcagggtctg
    11941 aacaaagcgg aaactgctga aaagtatggc gacgagcagg tgaaacagtg gcgtcgtggt
    12001 tttgcagtga ctccgccgga actgactaaa gatgatgagc gttatccggg tcacgatccg
    12061 cgttacgcga aactgagcga gaaagaattg ccgctgacgg aaagcctggc gctgaccatt
    12121 gaccgcgtga tcccttactg gaatgaaacc attctgccgc gtatgaagag cggtgagcgc
    12181 gtaatcatcg ccgcacacgg taactccctg cgtgcgctgg tgaaatatct tgataacatg
    12241 agcgaagaag agattcttga gctgaatatc ccgactggcg tgccgctggt gtatgagttc
    12301 gacgagaatt tcaaaccgct gaaacgctat tatctgggta atgctgacga gatcgcggcg
    12361 aaagcagcag ccgttgcgaa ccagggtaaa gcgaagtaaa cgtcattcgt ttaaaaaatg
    12421 agaaagccga ctgcaagtga gtcggctttt ttgtttgtcg ttgctaacaa tggagcacat
    12481 tgcctgatgc gacgctgcgc gtcttatcag gcctgtggtg attcatcgga tacgccactc
    12541 tgacggcgca tccgacaatt aaaccttacc cgcgacgcgc ttttactgca ttcaccagtt
    12601 gacgtaacag agcatcggta tcttcccagc caatgcaggc atcggtgatg ctcttaccgt
    12661 aggccagcgg ctccccgcta tcgaggctct gattgccttc caccagatgg ctttccacca
    12721 tcacgccaat aatggccttt tcgccaccgg caatctgctg gcaaacgtca gcacaaacat
    12781 ccatctgctt tttgaattgt ttggacgaat tagcatggct gaaatcgatc atcacctgag
    12841 ctggcagacc tgctttgttc agcccttctt tcacttcagc aacgtgcttc gcgctgtagt
    12901 taggctcttt accgccgcgc agaatgatat ggcaatcgcc gttaccactg gtattcacaa
    12961 tcgccgaatg cccccattta gttacggaca ggaagcagtg cggcgcaccg gcggcattaa
    13021 tggcatcgat agccacttta atcgtaccgt ctgtgccatt tttaaaacct accggacaag
    13081 aaagaccaga cgccagttcg cggtgcacct gcgattccgt ggtacgtgca ccaattgcgc
    13141 cccagctcat caggtcagcg agatactgag gagtgatcat atcgagaaac tcacccgccg
    13201 ctggcagacc gctgtcgtta atatcaagca gcaatttacg ggctatacgc agaccgtcgt
    13261 tgatctggaa gctgttatcc atatgcggat cgttaatcaa ccctttccag cccaccgtgg
    13321 tacgcggctt ttcaaaatag acgcgcatta cgatttccag ctcatctttc agctcttcac
    13381 gcagcgccag caagcgagtg gcatactctt tagccgcgac aggatcatga attgagcacg
    13441 ggccaatcac caccaacagg cgatcatcat tacctttcag gatcttatgg atcgcttttc
    13501 gggcatgggc aaccgtattc gcggcatttt cagttgcggg gaatttttcc agcaatgcga
    13561 caggaggaag taactctttg atttctttga tgcgtaaatc gtcgttctga taattcatgt
    13621 ctgttccagt gttgccatac ttatcttagt gaatgcaata cttccaatct atatcttccg
    13681 tcagaatgtg taaacggggt tttacactat gaacggatta atcctggaat tagcaagaaa
    13741 aacgccagat tgtcgcgaaa aacgagatct ctcctacaat ttctaactgt aactcctttg
    13801 tttgttaatt atttcaagat tctctgctgc gtttcataaa ctggctgaaa tcttaaacca
    13861 atgccttata ttcacctgca aatgcactgt tggaagaggt tatccgacat aacgaccata
    13921 acaggagcat cctatgaaaa tgacaaaact ggccacatta tttctgactg ccactctaag
    13981 ccttgccagc ggtgccgcac tggctgccga tagcggagcg caaactaata acggccaggc
    14041 aaacgccgca gctgatgcgg gccaggtagc ccctgacgcc cgtgaaaatg tcgcgccaaa
    14101 taacgtcgac aataacgggg taaataccgg ttctggcggc acaatgctgc attcggatgg
    14161 ttcttcaatg aacaatgacg gaatgaccaa agatgaagag cacaagaaca ccatgtgcaa
    14221 agatggtcga tgtccggata tcaataaaaa ggtacaaacc ggtgatggca tcaataatga
    14281 tgtcgatacc aaaaccgacg gtaccacgca gtaaccctgc aaaatagcca ataatgggag
    14341 agcggagtct ctccctttta atttttcact tctaaaagca gtatgatgtc ataaagttaa
    14401 gtgagaataa ttaagggatg actcgtcatg gcgcactcac actcacacac gtcctcacac
    14461 ctgccagaag ataataatgc tcgtcgcttg ttgtatgctt tcggcgtgac tgccgggttt
    14521 atgctggtag aagtcgttgg tggttttctt tctggttctc tggcattgct ggccgatgcg
    14581 ggtcatatgt tgaccgatac tgccgccctg cttttcgccc tactcgccgt gcaattttca
    14641 cgtcgtcctc ccaccattcg ccacactttc ggttggctaa gactgaccac gctcgcggcg
    14701 tttgtgaatg ccatcgccct cgtggtaatt accattttga ttgtctggga ggcgatagaa
    14761 cgcttccgca cgccgcgtcc ggtcgaggga ggcatgatga tggcaattgc cgtggctggg
    14821 ttgctggcaa atatactttc tttctggtta cttcatcacg gcagcgaaga gaaaaacctc
    14881 aacgtacgag cagcggcact gcatgtgctc ggggacctgc tgggttcggt tggggctatt
    14941 attgccgcgt tgattattat ctggactggc tggacacctg ctgaccccat tctctcgata
    15001 ctggtgtcgc ttctggttct gcgtagcgcg tggcgactgt tgaaagatag cgtgaatgaa
    15061 ttacttgaag gtgcaccggt atcgctggat atcgctgaac tgaagcgtcg tatgtgccgg
    15121 gaaatcccgg aagtccgcaa tgtgcaccat gtacatgtat ggatggtagg cgagaagccg
    15181 gtgatgacgt tgcatgtgca ggtgatcccg ccacacgatc acgatgcctt gttggatcag
    15241 atccaacact acctgatgga tcactatcag attgagcaca ccaccattca gatggaatat
    15301 caaccttgtc ataggccaga ctgccacctg aatgaagggg tgtctggcca ttcacatcat
    15361 caccattaat gggacagcgc gcgtgagcct ctttcacgtg cgctgttgat ccacatccgg
    15421 ctgccgttga gcgcaataaa ggtcaggatg atgtactcca gagacatggc gtaaacgccc
    15481 tgaagtgcaa aaataacgac gctaatcacg ttaataatca cccacaacag ccagttttcc
    15541 acatacttac gcgtcatcag aatcattgcc acgatagata acaccatcat gcatgaatcc
    15601 cagaacggga aagcgtccgg ttgcagttca ggcatcacaa cctgtaatcc taatgcttgc
    15661 atgatcatga ctgccacgcg ggtcaaaaat gcaaacaccg gattgataaa taccgtcatc
    15721 agaccaatcg aaacaacgca aaccgccaac cagctgagtg ccttcggcaa tggcaaccag
    15781 cgaattttca actccgcctc gttctgactg gtttgtcgcg accacgcata ccagccgtaa
    15841 atattcgcgg caaagaaaaa cacctgtaat agcaggctgg catacagctg aatctgaaag
    15901 aaaataatgc caaacaaggt aacgttgatc aggccaaaga agtagttgct gatcttctcc
    15961 agactggcaa ggccaataca cagcaacccg gcgatcgtgc ctaccgcttc gatccatgag
    16021 agatcataac cgcccgcccc tattggtata tgtaccagga tattctgcac actaaaaaaa
    16081 tccatctttc ccccaaagcg taacgttacg ccttattatt cgttatccac gtagtgtagc
    16141 cgcaaaatcc agcatacgat tgagcggcac cagcgccctc tctcgcagcc tctcatcgac
    16201 atgaacctcg tgattacttc cttcctgttc taatgcctct gcgatagcct gaaggtcatt
    16261 catggccatc cacggacaat gcgcgcagct gcggcaggtt gcgccctcac ccgcggttgg
    16321 tgcttccagt aactctttat ctggcaccgc ctgctgcatt ttgtagaaaa taccccgatc
    16381 ggttgccaca ataagcctct gatgaggcaa tgcttttgca gcagcgatca gttgactggt
    16441 ggaaccgacc gcatccgcca tatcgacaat agcttgtggt gattctggat gcaccagtat
    16501 ggcagcatcc gggtattctt cttgcaagcg ggttaacgcc tgagtcttaa atgcatcatg
    16561 cacaatgcag gcaccctgcc agcataaaat gtctccaccc gtctgttttt gcacgtaacg
    16621 ccccagatgt ttgtcgggtg cccagatgat tttttcaccc aaactatcaa gatgatcaat
    16681 aagttcgacg gcaatgcttg aagttaccac ccaatctgcg cgcgctttta ccgcagcaga
    16741 agtgttggcg tagacgacga cagtacgatc gggatgggca tcgtaaaatg cgttaaattc
    16801 ttcaacaggg cagccgagat ccagtgaaca ctcaacctga agtgtcggca tcagaattgt
    16861 tttttccgga ctgagaattt tggcggtttc tcccataaat ctcaccccag cgactaacaa
    16921 agtagaagcg ggatgctttg caccgaagcg cgccatttcc agagaatcag aaatacagcc
    16981 accggtttct tctgccagtt gttgaatttc gggatcggta tagtagtggg caaccatcac
    17041 cgcattacgt tcttttagca gacgttttat cttctcgcgg taatacgctt tttcatcaat
    17101 gcttaacggc gtcggcttcg gggggaaagg ataaatcgcc gtgtctggat caaacattac
    17161 gctcatctta ccatctcgtt ttacaggctt aacgttaaaa ccgacattag cgtaacattc
    17221 gctgttatcg gtgtaatttg ttttatatgc taaacaagat agtcgaatgg tggggaaaag
    17281 tcactcgaat tacgcctaat tccaccgacg tagagttgct tgcacagtaa gctctgacga
    17341 tgtcatcagg tttacaaagt gaaattgagg atgcctgaat agcaaaaaag cgcctttagg
    17401 gcgctttttt gcattggtgg gtcgtgcagg attcgaacct gcgaccaatt gattaaaagt
    17461 caactgctct accaactgag ctaacgaccc gagtggtggg tgatgacggg atcgaaccgc
    17521 cgaccccctc cttgtaaggg aggtgctctc ccagctgagc taatcacccg atactgcgct
    17581 ggatactaca aactacttcc accgaaccca ccgtaattag tggtgggtcg tgcaggattc
    17641 gaacctgcga ccaattgatt aaaagtcaac tgctctacca actgagctaa cgacccggtg
    17701 gtgggtgatg acgggatcga accgccgacc ccctccttgt aagggaggtg ctctcccagc
    17761 tgagctaatc acccgacact acgctggata ctacaactaa ctatgttagt gggggtcgtg
    17821 caggatgact cgacttcgtc tcgcccttcg gcgccgttgc tgatgcaacg ttatccttca
    17881 cgttttacta ccgcttccac cttagcgatt ggtgggtcgt gcaggattcg aacctgcgac
    17941 caattgatta aaagtcaact gctctaccaa ctgagctaac gacccacttt ttcgttgctt
    18001 tcggtttgtt tgatatcccg tggcaacggc ggcatatatt actgatttca gacttgagcg
    18061 caacaaaaat ttcgatgcag atcactcaac tgcttatgat tcgcacgaca cgaccagaaa
    18121 taatgcgact tctggtcgtg tgttatgcat tacatcgcgt tcagacgttt ttgtgcctgt
    18181 ttagcgccat cagtaccagg gtatttactg ataacctgct ggtatacggc tttcgctttt
    18241 gcggtgtcac ctttgtcctg catgatgacg ccgactttaa acatcgcatc agcagccttt
    18301 ggtgacttcg gatagttttt cactaccgaa gcaaaatagt acgccgcatc atctttttta
    18361 cccttgttgt agtttaactg acccagccaa taattggcgt ttggcaggta agttgaatca
    18421 gggtaatttt tgatgaaatt ctgaaatgcc accattgcgt catcctggcg ggatttatcc
    18481 tgcaccagcg caatagctgc attgtaatcc gtgtttgcat cgccgctttt caccggcgcg
    18541 ccagcattcg cagtaccagc atcagctgtc ggcgtcgttg atgccgtcgc accgctttga
    18601 tcgctgctgg ttgattgcgc cgctgcacca ccgctgctga ggctgtcgat ctgcaacagg
    18661 atctgctttt gccgctccac gacctgattc agttgatact gattttcctg aatctgacca
    18721 cgcagggaat caatatcgga ttgattatca gaaagttgtt gctggagttg ggttaaaagc
    18781 tggctgtgag cattagaaat acgctcaagt tgagtgacgc ggtcttcgac cgagcctgag
    18841 ctgacactac tgattggtgc ctgagcaaaa gcggcccagg gggccgctat accaaccagt
    18901 aacgacagac tcaatagttg atgtctgaag ttactgctca tgcaattctc ttagtaaacc
    18961 agtaccgcac gacggttttt ggagtatgcc gcttcgtcat gacccagtac tgcaggtttt
    19021 tctttaccgt aagaaacgat ggagatctgg tctgcagaaa cgcctttacc ctgcaggtac
    19081 atcttaacgg cgttcgcacg acgttcaccc agggagatgt tgtattccgg agtaccacgt
    19141 tcgtccgcgt gaccttctac ggtgactttg taagacgggt tgctacgcag gaagtttgca
    19201 tgtgcatcca gcatttgagc gaagtcagaa cggatatcgt acttgtccag atcgaagtaa
    19261 acgatgttgt tctgctgcag ctgttgcatt tgcagacgag cctgctcttc ggaagacatg
    19321 ttgccgttgc cgccgttcgc atccatacca gtgccggcac ccagcatgcc ttcgctgccg
    19381 tcattgctgg cgttcttgtt ggaagaacat gccgcaattg ccataacagg cagagcaatc
    19441 atcagccctt tcagcacttt gttcagttgc atttcaatga ttcctttact attcaattaa
    19501 ttattatcac agatacggcg accaggcagg gaatttgacc tgtccatcag ttgccggaag
    19561 acgcgctttg aaacgcccat ctgtagaaac caaattcagc acggatccca tcccctgaga
    19621 agagctgtag attaccatag tgccgttagg tgccagactt ggcgtttcat ccaggaacgt
    19681 ggacgacaga acttgtacgc ctcccgttgc cagatcttgt ttggcaatgt gctgctgccc
    19741 accattggag ctgaccatta ccataaattt accgtcgctg ctgacatccg catcctggtt
    19801 ctgcgaacct tcccaggtaa tacgttgtgg cgcaccgccg ttgatattca ctttataaac
    19861 ctgcggacga ccggcctggt cagaagtaaa tgccaggttc tggctatccg ggaaccaggt
    19921 cggttcggta ttgttactgc gaccatcagt cacctggcgg atctgaccag aagccaaatc
    19981 cattacgtac aggttcagac taccggtttt cgacaaggcg aatgccagtt tgctgccgtc
    20041 tggcgagaat gcaggtgcac cgttgtgacg cgggaatgaa gccacctgac gtacagcgcc
    20101 atttgccagc gtctgaataa ccagcgcgga acgaccgctt tcgaaggtca cataagccag
    20161 tttagaaccg tctggtgacc acgccggaga catcagcggc tgcggtgaac ggtgaacgac
    20221 aaactggttg taaccgtcat agtcagatac gcgcagttca tacgggaact gaccgccgtt
    20281 ggtctgaaca acgtaggcaa tacgggtacg gaacgcacct ttaatgccgg tcagtttttc
    20341 aaacacttca tcactggcgg tatgaccagc ataacgcagc cactgcttgt tcactttgta
    20401 cgagttctga gcaagtacag tacccggtgc gccgccagtg tcaacaagtt gataagcaac
    20461 attgtaagaa ccatccggat tcggagtgac ttgaccgaca actacagcgt caatgcccag
    20521 tgcggaccat gcagctggtt gtacttcctg cgcactaccc ggctgctgtg gcagacgagc
    20581 gcgatctaac ggattaaatt taccgctgtt acgcaagtca gcagcaacga tgccgccaat
    20641 atcttcaggt gccgcaccag gccccgccca ctggaaagga acaacaccaa taggacgacc
    20701 ggaatctaca ccgctgtcga tcacaatgcg gacttcagca tgcagaactg atgcccacag
    20761 tatgagaaaa ccaaatgcta ctcgtaatgc ctgcttcatc atatctccct tatctggacc
    20821 gatggcccac gataatttag cagaatgtta acaaactaaa atacacaaaa ctaccagaac
    20881 cccgtggcaa ccggtgcctg atgttgaccg tccgaacagt caacatcgcg attacggttt
    20941 gaagtccaat ggcgcgtttt tgaacacttc atatactgcc tggcttggtg gtttcgggat
    21001 cttcgcaagt ttagctgctg ccaacgcagc ctgacaaagt gcgggatcgc caccttcagg
    21061 tttgatatcc agtaacatac catcgggtgc cagttttatg cgcagcgtac aggttttgcc
    21121 tgcataggac gatgcgtcat agaacttact ttcgatagca gatttaatct gcccggcata
    21181 gttattgata tcggcccctg atgcgccatt gtttttagta ttaccactcc cggcaggcga
    21241 agcattgttc cctttcgccc ctccccccgt tttcggtgca ttcttaccag agcttagctc
    21301 accgaaaata tcatctgcct ctgcggcagc ttttgctgca gcggcttttt cagctgctgc
    21361 ttttgccgct gccgcttttt tgtctgcggc ggctttttct gccgctgctt tcttatcagc
    21421 tgctgcctta tcagcagccg ctttttctgc tgcttttttg tcggctgcag ctttctctgc
    21481 tgccgctttc ttatcagctg cagccttttc agcagccgct ttcttctcag cttctgcctt
    21541 ggctttttca gcagcttcag ttgccgcttt ctttcttgct tcagctgcag ctgcttctgc
    21601 cgcttccgct ttcttcttca gtgccgcagc ggctacctcg gcttttttct gcgcttcggc
    21661 tgcggctttg gcggcttctg cttctgcttt tttcttcgcg tctgcagccg ctttcttcgc
    21721 tgcttcttcc gcagctttag catctgccgc cgctttcgct gccgccactt cagcttgctt
    21781 ctgctttaac tcggcctgtt ttgcggcttc ttcagcctgc tttttctgct cctgagccgc
    21841 taaccgctct ttctcaagtt gcttcaggcg ttcctgttca gccgcttgtt tctcacgcag
    21901 ttcttcagca gcctgctgtt ccttcatctt gcgctgttca tcagaacgct tcgcgcttga
    21961 ttcctggctt tgcatgcgtt tgtactgctc aactaccgca cctgaatcaa ccatgacagc
    22021 gtcgatggac gaaccaccgc cgcctccggc tgaagcttct atattctcat cgaacgaact
    22081 ccagatcagc gccgcaaata agatgacatg cagcactgct gaaataatta tcgcccgctt
    22141 gagcttgtcg ttttgttcgg ttgcctttga cactctcggt ttccaaaaac tgttcgcctg
    22201 ttacccgctc tctttcaagc aagggaaacg cagatgttta gataggctgc gtcattaaac
    22261 caaccgattt cacacccgca ctatgtaaca agttcagtgc tttaattatt tcatcgtaag
    22321 gcacatcttt tgcgccaccg atcagaaaga ccgttttcgg gttggccttg aaacggctgg
    22381 acacttccgc caccacctgc tctggtggta aacgctccag gcgatctttc tcaaccacca
    22441 cggtgtactg accaatacca gacacttcaa caatcactgg cggattatcg ttactgctca
    22501 ccgcctgtga ttcagtagcg tctggcagat cgacctccac gctctgggtg atgatgggcg
    22561 ctgtcgccat aaagatcagc aacagcacca gcagtacgtc cagcaacgga acaatgttga
    22621 tttcggactt gagatcgcga cgacctcgtc cacgcgctct ggccatggct taccccttgt
    22681 tgctctcgct aacggtaaac gcctggcggt gcagaatcgc ggtaaactct tccataaagt
    22741 tgtcgtaatt cagttccagt ttgtttacgc gctggttgag acggttataa gccattaccg
    22801 ccggaattgc ggcgaacaga ccaattgccg tcgcaatcaa cgcttctgcg ataccgggcg
    22861 caaccatttg cagtgttgct tgttttaccg ccccgagggc gataaaggcg tgcatgatcc
    22921 cccagaccgt accaaacaga ccaatatacg ggctgatgga gccaaccgta ccgaggaacg
    22981 gaatgtgcgt ttccagattt tcaagttcac ggttcatgga gatacgcata gcacgcgacg
    23041 ccccttccac tacggcttcc ggcgcatggc tattggcacg atgcaggcgc acaaactctt
    23101 tgaacccgct gtaaaagatt tgttccgaac cagtcagatt atcccgtttc ccctgactct
    23161 cttgatagag gcgagagagt tcgattccag accagaattt atcttcaaac gcttcggctt
    23221 cacgcgccgc tgcgttaaga atacgggttc gctggataat aatggcccag gatgcgattg
    23281 aaaaaccaat caaaatcaac atgataagtt taaccagaag gctagccttc aggaacaaat
    23341 caaggatatt catgtcagtc actgcttaaa ctccgcgaca atagacttgg gaagcgcacg
    23401 aggcttcatt ttgagtgggt caacgcaaac aaccagaacc tctgcttcat tcagcaaagt
    23461 attctcggcg ttgacaatac gttgcgtgaa aaccaaagag gtgccacgca ttgatgttat
    23521 ttcagtctgt atttcgagca tatcgtcgag ccgcgcaggt gcgtaatatt ccaccgtcat
    23581 tttacgtacc acaaaggcaa cgcgttcagc catcagcgcc tgctgactga agtgatgatg
    23641 acgcagcatc tctgtgcgtg ctctttcata aaaagcgacg taactggcgt ggtacaccac
    23701 accaccggca tcggtatctt catagtagac gcgaaccggc catcgaaaca gcgttgtatt
    23761 cactttacat cccggtaatg caacaaaagt tagagctttt aaacgctgct actatacgcg
    23821 cgcgattgag gtttgggaat gagggcaagt taagggcgcg taaaaattta tgggcctaga
    23881 gcggcccata attttttaaa gagaattctg atttaaaaga agaagaaaat cagcccgaca
    23941 atgagaacaa tatcggcaag caatgggcaa aaaatccctt gccaaagaac cttctgcgga
    24001 cgaaagccca cgccgtgaat cacaccagca cagacggccc acatcagcaa taaaccatgc
    24061 cagatttcca gttcactggt cttcgcggca aaacgagatg ggtcccaaaa catacatcct
    24121 gctaacagaa gcgccatcac gaaggaaagc gcccgcaggg ggcgcttgtc cattaccgca
    24181 tacaaggtcg cgataatctt actcatcaga tgtcttcttg accggctttg cctgattcga
    24241 cgtgttcaag cgccagcgcg gtgattaccc caaacgaaca ggcaagaagc gttcccagaa
    24301 tccatgcgaa ataccacatt tttagctcct tacttagtac agagagtggg tgttacgttc
    24361 aatatcttct ttggtgatac gaccgaacat tttccagtaa caccaggcgg tgtagagcag
    24421 aatgatcggt accagaacca ccgcaaccca ggtcatgacg ttaagcgtca gctggctgga
    24481 agttgcatcc cacattgtca gacttgcgtt catcatggtg ctggacggca tcacaaacgg
    24541 gaacattgcg ataccggctg tcaggatgat gcaggccagc gtcagggagg agaacacaaa
    24601 cgcccacgcg gctttatcca tacgtgcagt caggatggtc agcagcagca gaaccacacc
    24661 cagtgccgga atagcccaca gaattggcgt gttgttgaag ttaaccagcc atgcgccagc
    24721 ttcacgaacc acttctttat tcagtgggtt agaggctgcg taatggtcca ttgtcgattt
    24781 cacgacataa ccatcgatac cgtacatcac ccatacgcca gccagtgcga aacagaccag
    24841 tgtcaccagc gcagccacct gagccgttgc acgggtacgc aggtgcagtt cgcccacggt
    24901 acgcatttgc agataggttg cgccctgagt aatgatcatc cctacgctca ccacgcctgc
    24961 cagcaggccg aacgggttaa gcaactggaa gaagttaccg gtgtagtaca gacgcagata
    25021 ttcatcaacg ttgaacggta cgccctgcaa caggttaccg aacgctacac caattaccag
    25081 cggcggaacg aagctaccaa tgaagatgcc ccagtcccac atgttacgcc agcgggtttc
    25141 ttcaatcttg gagcggtagt caaaaccgac cggacggaag aacaaagacg ccagcacgag
    25201 gatcatcgcc acatagaagc cggagaacgc agcggcatag accatcggcc aggcagcaaa
    25261 gagtgcgccg cccgcggtga tcagccaaac ctggttaccg tcccagtgtg gtgcaatgga
    25321 gttaatcata attcgacgct cggtgtcgtt acgaccgagg aaacgggtga gcatgcccac
    25381 ccccatgtcg aaaccgtcag tgactgcaaa accaatcagc agaacgccaa ccagcagcca
    25441 ccagataaaa cgcaatactt cataatcgat catttgacga ctcctgtctt agcgtgccgg
    25501 ctgagtagtc gtggaagact gctcaaagtg atagcgaccg gttttcaggc tgcttgggcc
    25561 gaggcgtgca aacttgaaca ttaagaacaa ttctgccacc aggaacaggg tatacaggcc
    25621 gcaaatcagc accattgaga agatgagatc gcctgcggtc agtgacgaat tcgccacagc
    25681 tgtcggcagc acttcaccga tagcccacgg ttggcggcca tattcagcca cgaaccagcc
    25741 cgcttctaca gcaatccacg gcagcggaat accgtacagc gcggcgcgca gaagccattt
    25801 tttctcgcca atgcggttgc ggatgacact ccagaaagag agcgcgatga ttgccagcag
    25861 caggaagcca cacgccacca tgatacggaa cgcaaagtac agcggcgcta cacgcgggat
    25921 ggagtctttg gttgcctgtt gaatctgcgc ttcagtcgca tcagccacgt ttggcgtata
    25981 gcgtttcagc agcagaccgt aaccgaggtc tttcttcatg ctattgaact ggtcacgaac
    26041 cgtctggtcg gtagaaccag aacgcagttg ttcgagcaga gagtacgcct tcatcccgtt
    26101 acgaatgcgt tcttcatgct gcaccatcag ctctttcagg ccgataaccg gggtatccac
    26161 ggaacgcgtt gcaatgatgc ccagtgcgta agggatctga atcgcaaatt tgttcgtctc
    26221 ctcttcctga tcaggaatgc cgaacaaagt aaaggcagca ggcgcaggtt gcgtttccca
    26281 ctcggcttca atagcagcca gtttggtttt ctgcacgtcg cccatttcgt agccggattc
    26341 atcacccaga acaataacag acagaacagc agccataccg aagctggcag cgatagcaaa
    26401 ggagcgttta gcgaaggcga agtcacgacc tttcagcata taccatgcgc tgataccgag
    26461 gatgaacatc gcgccagtca cataaccaga cgctacagtg tgaacgaatt tcacctgagc
    26521 aaccgggtta agcaccagct cggagaagct caccatctcc atacgcatag tttcaaagtt
    26581 gaaatcggac gcgattgggt tttgcatcca gccgttcgca accagaatcc acagtgcgga
    26641 caggtttgaa ccgagcgcca ccagccaggt gacacacata tgctgaactt tacccagacg
    26701 atcccaaccg aagaagaaca gacctacaaa ggtggattcg aggaagaagg ccatcagacc
    26761 ttcgattgcc agcggcgcac cgaagatatc ccctacatag tgggaatagt aagaccagtt
    26821 agtcccgaac tggaactcca tggtcagacc ggtagccaca cccagagcga agttgatacc
    26881 aaacaacttg ccccagaact tggtcatatc tttataaatc tgtttgccgg agaggacgta
    26941 gaccgtttcc ataatggcca ggaggaacgc cataccgagc gtcagtggca caaaaaggaa
    27001 gtggtacatc gcggtcaagg caaactgtaa gcgcgacagt tcgactatat ctaacatcat
    27061 gactccttgc tcatcgcatg aagactccga gagtgaaccc cgttagaaag ggtcacacgc
    27121 atgccccaat agaaatttgt ttgaacactt tcatcatacc atgtgaatgc acacaaaaac
    27181 ggcgtaaaga aaggttgcaa acatgttaat aaaaactcaa attgatccca cgtatatatt
    27241 acgccgcaaa atccttacaa taaacaggtt tttgttgaag caaatttgca tttttcgacg
    27301 gtgatcaatt tatagctaaa ttgccgcctt tcagccaatt tgatcgagaa caatttatct
    27361 ctttttgatg c
//
LOCUS       DAEBXN010000052        27066 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.052, whole genome shotgun sequence.
ACCESSION   DAEBXN010000052 DAEBXN010000000
VERSION     DAEBXN010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27066
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.052"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..470
                     /locus_tag="KJL99_002249"
     CDS             <1..470
                     /locus_tag="KJL99_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
                     /protein_id="HBD4436962.1"
                     /translation="GDITYLRTDEGWLYLAVVIDLWSRAVIGWSMSPRMTAQLACDAL
                     QMALWRRKRPRNVIVHTDRGGQYCSADYQAQLKRHNLRGSMSAKGCCYDNACVESFFH
                     SLKVECIHGEHFISREIMRATVFNYIECDYNRWRRHSWCGGLSPEQFENQNLA"
     gene            complement(467..607)
                     /locus_tag="KJL99_002250"
     CDS             complement(467..607)
                     /locus_tag="KJL99_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005061990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBD4436963.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGHSDPTHVIWT
                     QA"
     gene            849..1142
                     /locus_tag="KJL99_002251"
     CDS             849..1142
                     /locus_tag="KJL99_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HBD4436964.1"
                     /translation="MFCAIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            1214..1873
                     /locus_tag="KJL99_002252"
     CDS             1214..1873
                     /locus_tag="KJL99_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HBD4436965.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEKRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1965..2411
                     /locus_tag="KJL99_002253"
     CDS             1965..2411
                     /locus_tag="KJL99_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HBD4436966.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(2618..3518)
                     /gene="hlyE"
                     /locus_tag="KJL99_002254"
                     /pseudo
     CDS             complement(2618..3518)
                     /gene="hlyE"
                     /locus_tag="KJL99_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            3891..4310
                     /locus_tag="KJL99_002255"
     CDS             3891..4310
                     /locus_tag="KJL99_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HBD4436967.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            4310..5578
                     /gene="umuC"
                     /locus_tag="KJL99_002256"
     CDS             4310..5578
                     /gene="umuC"
                     /locus_tag="KJL99_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HBD4436968.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVMLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(5624..6154)
                     /gene="dsbB"
                     /locus_tag="KJL99_002257"
     CDS             complement(5624..6154)
                     /gene="dsbB"
                     /locus_tag="KJL99_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HBD4436969.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(6300..7841)
                     /gene="nhaB"
                     /locus_tag="KJL99_002258"
     CDS             complement(6300..7841)
                     /gene="nhaB"
                     /locus_tag="KJL99_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HBD4436970.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYNVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            8062..8781
                     /gene="fadR"
                     /locus_tag="KJL99_002259"
     CDS             8062..8781
                     /gene="fadR"
                     /locus_tag="KJL99_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HBD4436971.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(8833..10366)
                     /locus_tag="KJL99_002260"
                     /pseudo
     CDS             complement(8833..10366)
                     /locus_tag="KJL99_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707085.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
     gene            10696..11994
                     /gene="dadA"
                     /locus_tag="KJL99_002261"
     CDS             10696..11994
                     /gene="dadA"
                     /locus_tag="KJL99_002261"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HBD4436972.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVDPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKFGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGSTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            12004..13074
                     /gene="dadX"
                     /locus_tag="KJL99_002262"
     CDS             12004..13074
                     /gene="dadX"
                     /locus_tag="KJL99_002262"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HBD4436973.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQSDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGAPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(13242..14656)
                     /locus_tag="KJL99_002263"
                     /pseudo
     CDS             complement(13242..14656)
                     /locus_tag="KJL99_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243131.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(15267..17003)
                     /locus_tag="KJL99_002264"
     CDS             complement(15267..17003)
                     /locus_tag="KJL99_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HBD4436974.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(17098..18012)
                     /gene="ldcA"
                     /locus_tag="KJL99_002265"
     CDS             complement(17098..18012)
                     /gene="ldcA"
                     /locus_tag="KJL99_002265"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HBD4436975.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINVHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGAQLTISGHPVLKM"
     gene            18112..18723
                     /gene="emtA"
                     /locus_tag="KJL99_002266"
     CDS             18112..18723
                     /gene="emtA"
                     /locus_tag="KJL99_002266"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HBD4436976.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(18725..19459)
                     /locus_tag="KJL99_002267"
     CDS             complement(18725..19459)
                     /locus_tag="KJL99_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HBD4436977.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISTSERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            19660..19914
                     /locus_tag="KJL99_002268"
     CDS             19660..19914
                     /locus_tag="KJL99_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBD4436978.1"
                     /translation="MGIIAWIIFGLIAGIIGKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIDGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            complement(19964..21424)
                     /locus_tag="KJL99_002269"
     CDS             complement(19964..21424)
                     /locus_tag="KJL99_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HBD4436979.1"
                     /translation="MHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMI
                     NSSADDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNCGQKSRYWQWPEYDKE
                     SFYYQGATQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQ
                     LAADVRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVA
                     GISYDWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRF
                     PTLKERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTRDWGFEVSVYYNRVSDA
                     ILSHNINADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKIT
                     DLPTQTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHG
                     FSVNASVNNLFDTQYAYSEGFIEEGRNFWAGVEYTF"
     gene            complement(21361..21918)
                     /locus_tag="KJL99_002270"
     CDS             complement(21361..21918)
                     /locus_tag="KJL99_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HBD4436980.1"
                     /translation="MRLKKRYLCTALSLAFTQQAVAAQESDTLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLHGPNQMGGAINITTLKKQVWDIARDGAVVR
                     TMPTICMLHLPPAAILGICKSAVAS"
     gene            complement(21944..22798)
                     /locus_tag="KJL99_002271"
     CDS             complement(21944..22798)
                     /locus_tag="KJL99_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HBD4436981.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQPGYIAFFHRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQGWSGAIAQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPTASI"
     gene            complement(22798..23607)
                     /locus_tag="KJL99_002272"
     CDS             complement(22798..23607)
                     /locus_tag="KJL99_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBD4436982.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLIAKIDLQGAQTLFDMGCGPGTVSLALADKLTTVYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRHAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYHWYQQQDARALAPASRDWALIWWDSVPQEAL"
     gene            complement(23617..24375)
                     /locus_tag="KJL99_002273"
     CDS             complement(23617..24375)
                     /locus_tag="KJL99_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBD4436983.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLSVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHTLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISR"
     gene            complement(24372..25352)
                     /locus_tag="KJL99_002274"
     CDS             complement(24372..25352)
                     /locus_tag="KJL99_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBD4436984.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENESV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACAA
                     ILWGLPIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWIGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            complement(25352..26374)
                     /locus_tag="KJL99_002275"
     CDS             complement(25352..26374)
                     /locus_tag="KJL99_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HBD4436985.1"
                     /translation="MPITRRTFAQALASTLLLQSLPSFSQTVNRFASQSLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETLISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASHFVGEAQAFATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
     gene            26724..>27066
                     /locus_tag="KJL99_002276"
     CDS             26724..>27066
                     /locus_tag="KJL99_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4436986.1"
                     /translation="MLGVIQNLCCCRPDSPLADSKQRNQKLPQQDFEDFKNDVINVIN
                     NNEDIYFLRESNLLNAFIRTCKKRDDNSYGNMIFTLYFDNDIYGNRIIKASLNGGELQ
                     LLSTGCSNDNIL"
BASE COUNT         6793 a         6586 c         7006 g         6681 t
ORIGIN      
        1 caggagacat cacgtactta cgtacagatg aaggctggct gtatctggca gtggtcattg
       61 acctgtggtc acgtgccgtt attggctggt caatgtcgcc acgcatgacg gcgcaactgg
      121 cctgcgatgc cctgcagatg gcgctgtggc ggcgtaagag gccccggaac gttatcgttc
      181 acacggaccg tggaggccag tactgttcag cagattatca ggcgcaactg aagcggcata
      241 atctgcgtgg aagtatgagc gcaaaaggtt gctgctacga taatgcctgc gtggaaagct
      301 tctttcattc gctgaaagtg gaatgtatcc atggagaaca ctttatcagc cgggaaataa
      361 tgcgggcaac ggtgtttaat tatatcgaat gtgattacaa tcggtggcgg cggcacagtt
      421 ggtgtggcgg cctcagtccg gaacaatttg aaaaccagaa cctcgcttag gcctgtgtcc
      481 atattacgtg ggtaggatca ctatggcctt tgctgaactc aacattgaca ttcttacccg
      541 cagcaaagct cgtggaggtt agcagtgcaa gcagcaatac agccttactg atgctcttga
      601 ttttcattgg tagtctcctg ttcagaatga gtacgatgaa ctgttataat ataacaatcc
      661 cttacgggag aattccagtg gcttttctgc gttcggtaaa atcccggcgc ggcatttttt
      721 gccgggggga ttacttataa aggggcatgg actcaacaaa ccgcagaaat ctacagctgg
      781 ctggcttgtg gcgcgggttt cattgatgtt agcttatgcc aaaacctggc atcttaaaga
      841 gtaaaagtat gttttgtgcg atttatcgaa gcagcaagcg tgaccagacc tatttatatg
      901 tcgaaaaaaa agacgatttt tcgcgtgttc ctgaggaact gatgaaaggt tttggtcagc
      961 ctcagttagc gatgattctg ccgttggatg ggcggaagaa actggtcaat gctgatattg
     1021 aaaaggttaa actggcatta accgagcaag gttactattt gcagttaccg ccaccacccg
     1081 aagatttgct gaagcaacat ctttccgtca tggggcagaa aacagacgac actaacaaat
     1141 aaccgatatc cggcggtggc attatctttg tcggcgcggg ttttcatatc cacgataagg
     1201 tgaggggaac gttatgtatc aacatcacaa ctggcaaggt gcgctgctgg attatccggt
     1261 gagtaaagta gtctgtgttg gcagtaacta tgccaaacat attaaagaga tgggcagcgc
     1321 agtgcccgaa gagccagtgc tgtttattaa accagaaacg gcactgtgcg atctgcggca
     1381 gccattggcg atcccatccg atttcggttc agttcatcat gaagtcgaac tggcggtgtt
     1441 gattggcgcg acgctgcgtc aggcaacgga agagcatgtc cgcaaagcca ttgccggtta
     1501 cggcgtggcg cttgatctga cattgcgtga tgttcaggga aaaatgaaga aagccgggca
     1561 gccgtgggaa aaggctaaag cgtttgataa ctcttgtccg ctttccgggt ttatccccgc
     1621 ggcggaattt accggcgatc cacaaaatac aacgctgggc ctgagcgtaa acggcgaaaa
     1681 acgccagcaa ggtacgactg cggacatgat ccataaaatc gttccgttga tcgcttatat
     1741 gagcaagttt tttaccctca aggccggtga cgttgtgttg acaggcacgc ctgatggcgt
     1801 cggcccgttg caaagcggtg atgagctgac agtcactttc gatgggcatt ctttgacaac
     1861 tcgcgttttg taatactttt tgccgccaga aagcggcggc aaaacttgca tcgctgtgcc
     1921 agactggtta taaggtgcgt tttaacgtaa tggcggaaca cctgatgagc gatgtacctt
     1981 tctggcaaag taaaaccctg gacgaaatga gcgatgcgga atgggagtcg ttgtgtgatg
     2041 gttgcggtca gtgttgcttg cataaactga tggatgaaga caccgacgaa atctacttca
     2101 ctaacgtcgc ctgtcgccag ctcaatatta aaacctgtca atgtcggaac tacgaacgtc
     2161 gtttcgagtt tgaacccgac tgcattaaat taacccgtga aaatctgcca acattcgaat
     2221 ggctgccaat gacctgcgct tatcggttgc tggcggaagg taaagattta cctgcgtggc
     2281 atccgctact tactggttcg aaagcggcaa tgcatggtga acgtatctct gtgcgtcata
     2341 tcgcagtgaa agaatcagaa gtgattgact ggcaggatca tatcttaaat aaacctgact
     2401 gggcgcagtg ataaatttaa tagatgctga tgggggcttt cttaatatac gtctggatgc
     2461 tcgccatttc gtagccctgt ttttgaaagc gctctgtcat aatgagagtt acccgatacc
     2521 ttattatcaa caggtaactc tcaatcttta aaaaacgtct gtgtttttaa atgtgataaa
     2581 ccttatacct tgagtacatg gagagaataa tcgcttatca gacttcaggt acttcaaaga
     2641 gtgtcttttt accgtgtctt ttctgatact cattacaggt gttaatcatt tttttggccg
     2701 ctgcttttag caaagaaagc attaaatcat cataatcaac atagaatctg gttgtttcag
     2761 tttccgtttt tatctccccg atggcggcta tttcggtggt taatttcaat ttggcggcat
     2821 cgatatcttt attcgcttgt ttaaccgtgt tagacagggt ggtaaagaaa ctctgcacag
     2881 attttaactt gttcttcaat tctggaatca gtttcccttc aactacgccc gcagcaatag
     2941 aataggaaat gattaaacca aatggaccgg cgacgacatc ggctgcggca ccggcatacg
     3001 cttccttcct gattttatct acctgtgact ggaaatagct gcttttttct gaaaaatcat
     3061 tggttaactg gctatctaac gccagcagtt tcccggaagc gttgttgaaa ctttgtgagc
     3121 ttaccagcag ggatttttgc gcttcattca gcttcgtgat gccgtcatcc agtaccttaa
     3181 tgaggggcgg atgctttctt ctcattgtac tcatcaaata gcaaaatata cgctgcgagc
     3241 aattgcgtcg caacaccaca ccattcatac actgtttggg ttgcttcaaa atacttatcc
     3301 tggctatcca taagtaaggt tttaatatcg ccgactaaaa cggaggctgc ctgtgaatac
     3361 tcctgtttaa agcgacttaa ctcttttatg gtttcatcaa aggtctgcca ggggatgacc
     3421 tgatcgagat atttattata aagatctaat gctccatctg cggtttcgat tgcgttttta
     3481 actacttcta ccgttttatc tgcaacgatt tcagtcataa tcattcgcct ctttaaatat
     3541 ataaattgta atgaaactcc tgttttacaa ctattaataa attttacttc atctaattca
     3601 tagttagccg ggcgggatgc gtcaatgtct ttatttctat taatatgata aatatcaaac
     3661 aatgtttaat gtcattatgg cgaatgcttc tattctattt ttttgccggg tgatattttt
     3721 catttctgct ggatgagcgt cgtcgccaga aggccacgtg agcacaggat aagagaacga
     3781 aaaatcagca gtctatgcag cgacaaatat tgatagcctg aatcagtatt gatctgctgg
     3841 caagaacaga ccactgtata taaaaacagt ataacttcag gcagattatt atgttgttta
     3901 tcaagcctgc ggatctccgc gaaattgtga cttttccgct atttagcgat cttgttcagt
     3961 gtggctttcc ttcaccggca gcagattacg ttgaacagcg catcgatctg aatcaactgt
     4021 tgatccagca tcccagcgcg acttacttcg tcaaagcaag tggtgattct atgattgatg
     4081 gtggaattag tgacggtgat ttactgattg tcgatagtgc tattaccgcc agccatggtg
     4141 atattgtcat cgctgctgtt gacggcgagt ttacggtgaa aaaattgcaa ctacgcccga
     4201 cggtacagct tattcccatg aacagtgcgt actcgcccat taccatcagt agcgaagata
     4261 cgctggatgt ctttggtgtg gtgatccacg tcgttaaggc gatgcgctga tgtttgccct
     4321 ctgtgatgta aacgcgtttt atgccagctg tgagacggtg tttcgccctg atttatgggg
     4381 taaaccggtg gttgtgctat cgaataatga cggttgcgtt atcgcccgaa acgctgaggc
     4441 aaaggcgctg ggcgttaaaa tgggcgatcc ctggttcaaa caaaaagatc tgtttcgtcg
     4501 ctgtggtgtg gtttgcttta gcagcaatta tgagctttac gcagacatga gcaatcgggt
     4561 gatgtcgacg ctggaagaac tatcgccccg cgtcgagatt tacagtattg atgaagcatt
     4621 ctgcgatctg acaggtgtgc gtaattgtcg cgatctgact gattttggca gagaaattcg
     4681 cgcaacggtg ctacaacgta cccatcttac tgttggtgtg gggatcgccc agaccaaaac
     4741 gttggctaag cttgccaatc atgcggcaaa aaaatggcag cggcagacgg gtggggtggt
     4801 ggatttatca aatctggaac gccagcgtaa attaatgtct gctctccccg tggatgacgt
     4861 ctgggggatt ggacggcgga tcagcaaaaa actggacgcg atggggatca aaaccgttct
     4921 cgatttggcg gatacagata tccggtttat ccgtaaacat tttaatgtca tgctcgaaag
     4981 aacggtgcgt gaactgcgcg gcgaaccctg tttgcaactg gaagagtttg caccgacgaa
     5041 gcaggaaatt atctgttccc gctcgtttgg tgaacgcatc acggattata cgtcgatgcg
     5101 gcaggccatt tgtagttacg ctgcccgggc ggcggaaaaa cttcgcagtg agcatcaata
     5161 ttgtcggttt atctccacat ttattaagac gtcaccattt gcgctcaatg aaccttatta
     5221 cggcaatagc gcgtcggtaa aactgctgac gcccactcag gacagcaggg atatcattaa
     5281 cgctgctacg cgatctctgg atgccatctg gcaagcgggc catcgctacc aaaaagcggg
     5341 cgtgatgctg ggggatttct tcagtcaggg agtcgcgcag ctcaatttat tcgatgacaa
     5401 cgcaccgcgc cccgggagtg agcaattgat ggcggtaatg gatactctga atgctaaaga
     5461 gggcagagga acactctatt ttgccgggca ggggatccaa caacaatggc agatgaagcg
     5521 agctatgctt tcaccacgtt atacaacgcg aagttctgat ttactgcggg tcaaataaat
     5581 atagcggcag gaaaaaagcg ctcccgcagg agcgccgaat ggattagcga ccgaacagat
     5641 cacgtttttt cgctttaaac ggctgggaaa tcaccaccag caccgcgaca atcaggtaag
     5701 cgataaaaat accgagcagc cactgcggca tttccatacc taaaaaatcc cactgacgct
     5761 cggcgcaatc gccagaggcg acaaacactt gcggcaccca tttatccagc ggcagccatt
     5821 ccgggaaacg aaccataaaa tcacaggtgg caaatggcga aggatagagc tgaagcatgg
     5881 tgtgctcgta agttaactgc acaccgcgga acgcactata caaccagata accatcgcta
     5941 cataacgcag cggagttttc ggggcgatcg cgccaatcag cgcagcaccc agaacgccga
     6001 ataacgcgca gcgttcataa atacagagca cgcaaggttt cagtaacatc acatgctgga
     6061 accacagcgc cgtcagttcc agtgccagag cagtaaacgc catcaacagc cacgcgcccc
     6121 ggccttgtga acattggttc aaaaatcgca acataatcat ttccctgcaa tatgcataga
     6181 gtgcgcagtt taaaccaatt cattcgctgc gccaccagga ggcagcaaaa ataggacgca
     6241 attggacaat tatcgggcga aaaggcaaac cgggcattta tatgcccggt aagttgttat
     6301 caaagcgttg ctatccagcc catttgcata aaccattcgg ttacaggggc aagcgtaaac
     6361 tcgacgcaga gcaagccgac gagtgtcagt acgagggtgt aaggcagggc catccacacc
     6421 atgcggccat aagagaggcg aatcaatggc gcgagtgcag aggtcagcag gaacaggaac
     6481 gcagcctgac cgttcggcgt agcgacagag ggcagattgg taccggtatt aatggcgacc
     6541 gccagcagct cgtattgctt caacgtgata gcgccacttt ccattgccgc tttcgcttcg
     6601 ttgatataaa tcgtccccac gaagacgtta tccgaaatgg atgacagcag accgttgaaa
     6661 atatagaaca gcgacagctg agcatgttca gatgcctgca acacaaactg aataatcggc
     6721 gaaaacagtt gttggtcgat aatcaccgcg actaccgaga aaaacaccgt caacagtgcg
     6781 gtgaatggca gagattcggt gaaggcttta ccgatagcat gttcatcggt gacaccggtc
     6841 aatgatgttg ccagaataat gactgacaaa ccaatcaagc caacttccgc cagatgcaac
     6901 gccagcgcag tcaccagcca gacgccaata atcgcctgga caatcagacg gattttatcc
     6961 tgacgggtac gctggtggcg gctttgatcg tcaaactgtt gcaacacttc gcggactttc
     7021 tccggcagcg tttcaccgta gccaaaccaa cgcagcttct ctaccagcag gcaggttaac
     7081 aggccacaaa tcagaaccgg aacggtcacc ggcgacatgc gcaggaagaa atcgccaaaa
     7141 tgccagccag ccgctttagc gatgatcagg ttctgtggtt cgcccaccat ggtcattacg
     7201 ccgcctaatg cggtgccgac accggcatgc atcatcaggc tacgcagaaa gccacggaac
     7261 tgttccagaa ccacgttgta atgcttgtcg atatgactat cgtcttgcag gtcggtgtct
     7321 tcggtacggg aagaggctac gcgatgataa ataccataaa aaccgactgc aacgctgatc
     7381 accaccgcca cgacggttaa ggcatcgagg aacgcggaga ggaacgctgc cgccacgcaa
     7441 aaagagagcg acagcagcat tttggagcga atgcttaaca gcaaacgggt aaatatgaac
     7501 agcaacagct gtttcataaa atagataccc gccaccataa acatcaataa cagcaagact
     7561 tcaagatttg ccgccacctc ttcacggacg tgttccgcgc tggtcatgcc gatgaatacc
     7621 gcttcgatag ccaacagacc accggggagt agcgggtagc atttcagggc catcgccaga
     7681 gtgaaaataa attccgctac cagcaaccag cccgcgacga aagggctgat gaggaaaatt
     7741 aacgggttta cgattaagaa aatgatgagg gcgagtttgt accagtcggg ggactggccc
     7801 aaaaaattgc gccatagcgc gcggccccag gagatctcca tgatggtttc ccttacctta
     7861 caaataatca atgatgtttt tatgtttaaa cgcaaagctt aacggtcagg caggagtgag
     7921 gcaagtcttg atagtcaagg ggaaagagat gcggaaaatg aagccttgat ccctttttct
     7981 tctttttgtc tgctatcagc gtagttagcc ctctggtatg atgagtccaa ctttgttttg
     8041 ctgtgttatg gaaatctcac tatggtcatt aaggcgcaaa gcccggcggg tttcgcggaa
     8101 gagtacatta ttgaaagtat ctggaataac cgcttccctc ccgggactat tttgcccgca
     8161 gaacgtgaac tttcagaatt aattggcgta acgcgtacta cgttacgtga agtgttacag
     8221 cgtctggcac gagatggctg gttgaccatt caacatggca agccgacgaa ggtgaataat
     8281 ttctgggaaa cttccggttt aaatatcctt gaaacactgg cgcgactgga tcacgaaagt
     8341 gtgccgcagc ttattgataa tttgctgtcg gtgcgtacca atatttccac tatttttatt
     8401 cgcaccgcgt ttcgtcagca tcccgataaa gcgcaggaag tgctggctac cgctaatgaa
     8461 gtggccgatc acgccgatgc ctttgccgag ctggattaca acatattccg cggcctggcg
     8521 tttgcttccg gcaacccgat ttacggtctg atccttaacg ggatgaaagg gctgtatacg
     8581 cgcattggtc gtcactattt cgccaatccg gaagcgcgca gtctggcgct gggcttctac
     8641 cacaaactgt cggcgttgtg cagtgaaggc gcgcacgatc aggtgtacga aacagtgcgt
     8701 cgctatgggc atgagagtgg cgagatttgg caccggatgc agaaaaatct gccgggtgat
     8761 ttagccattc aggggcgata atcccttccg tttaaagagc aaccccctca aacgaggggt
     8821 tttttgttgt ttttacagat ttcccattct tggcgggcaa cgttccagca actcgatgct
     8881 gccgtcttcg ttttgctgtt cgagcatcac atcaaatccc cacaggcgat gcacatgctt
     8941 caggacttct ttgcgccccc gatccagcgg tgcgcgatta tgtggaatat aacgcagcgt
     9001 cagcgaacgg tcgccgcgca aatccacgtt ccagatctga atattcggct ccagattact
     9061 taagttatat tgcgacgata accggttacg gatctcccga taaccttctt cattatgaat
     9121 agcggaaatc tccagataat tatgccgatc atcatccagc acggtgaaga agcggaaatc
     9181 acgcatcact ttcggtgaca gaaactggct gataaaactc tcatctttga aatcacgcat
     9241 cgcgaaatgc agcgtttcca gccagtcgga accggcgata tccgggaacc agtatttgtc
     9301 ttcttccgtt ggcgactgac aaatccgttt aatatcctgg aacatggcga acccgagggc
     9361 atacgggttg atgccgctgt accacgggct gttatagggg gggctggaag accacattgg
     9421 tgtggctgtg caaaaactcc agcataaaac gttccgttac tttcccttca tcatacagat
     9481 ggttaaggat ggtatagtgc cagaaggtcg cacagccttc gttcatcacc tgagtctgtt
     9541 tttgcggata aaaatactgg ctcaccttac gcacaatacg caggatttca cgctgccatg
     9601 attccagcag tggcgcattt ttctccataa aatagagcag gttttcttgt ggttcggacg
     9661 gatagcggcg cgcttcagca accgttttct cttcctcgcg cttcggcagg gtacgccaga
     9721 gcatattgac ctgactttgt agatactctt cgcgactttt ctgccgggct ttctcttctt
     9781 gcagcgagat tttttgcggg cgtttgtacc ggtccacgcc gtagttcatc agcgcgtggc
     9841 acgagtccag aagccgttct acttcatcaa cgccataacg ttcttcgcac tcggtaatat
     9901 atttacgggc aaaaatcaga taatcgacaa tcgaactggc gtcggtccaa ctgcggaata
     9961 agcaattgtt tttaaagaaa gagttatgtc catagcaggc gtgtgccatc accagcgcct
    10021 gcatagtaat ggtgttctct tccatcaagt aagcgataca cgggttagag ttaatgacga
    10081 tttcataggc cagcccttgc tgaccgtgct tatacagccg ttcagtctcg ataaactttt
    10141 taccgaatga ccagtgcgga tagttaattg gcatgccgac gctggaatag gcatccatca
    10201 tctgttctga ggttatcact tcaatctggt gcgggtaggt atccagccgg tagagtttcg
    10261 ccacccggtc tatctctgcc agataaacat ccagcaggtc gaacgtccag tcgggtccat
    10321 cgctcaaacg tgtggtgtcc ttattcatag aatcgatcgt cgccatacgc gcacctcatt
    10381 gttgtcggcg ctctctgtgt ggagcacctc atttcaagca tagaacacct gttaaaaacc
    10441 gcgtcgccgg agaatttttt tctttgcgat ttcttattat cagagtgcca ctaatccgct
    10501 tctgaacgga attttatgct ggataaaaag ggcgttcagc aggagatact aaagacgcca
    10561 tattgccgca gagtcaggga gatgtgagtc agctcaccat aaaaaagccg tatgttgaat
    10621 aatattttca actgagttat caagatgtaa ttagattatt attcttttac tgtatctacc
    10681 gttatcggag tggctatgcg agttgtcata ctgggaagtg gtgtggtagg cgttgccagc
    10741 gcctggtact taaatcaggc aggacatgag gtcaccgtca ttgatcggga gccgggggcc
    10801 gccctggaaa ccagtgccgc gaatgccggg caaatctccc ccggatatgc tgcaccgtgg
    10861 gcagcaccag gtgtgccttt aaaagcgatt aaatggatgt tccagcgcca tgcgccgctg
    10921 gcggttcgtc tcgacggtac gcagtttcag ttgaaatgga tgtggcaaat gttacgtaac
    10981 tgcgacacca gccactacat ggaaaacaaa gggcggatgg tgcgtctggc ggaatacagc
    11041 cgtgattgcc tgaaagcatt gcgcgccgaa accaatattc agtatgaagg gcgtcagggt
    11101 gggacgctgc aactgttccg taccgaacaa cagtatgaaa atgcgacccg cgatatcgcc
    11161 gtgctggaag atgccggcgt accgtatcag ctgctggaat ccagccgcct ggcggaagtg
    11221 gatcccgcgc tggcagaagt agcgcacaaa ctgacgggcg gcctgcagtt acccaatgat
    11281 gaaaccggag actgccagct gtttacccag aatctggcgc ggatggcaga gcaggcgggg
    11341 gttaaattcc gctttaatac gcccgttgac caactgcttt gcgacggcga gcaaatctac
    11401 ggcgtgaagt ttggcgatga agtgattaag gccgatgcgt atgtgatggc gtttggttct
    11461 tactcgacgg cgatgctcaa aggcattgtt gatattccgg tgtatccgct gaaaggctac
    11521 tcgctgacta ttccaattgc gcaggaagat ggtgcgccag tatccaccat tcttgatgaa
    11581 acctacaaaa tcgccattac ccgtttcgat aaccgcattc gtgttggcgg aatggcggaa
    11641 attgttggtt ttaacaccga gctgttgcaa ccgcgtcgtg aaacgctgga aatggtggtt
    11701 cgcgatctct atccacgcgg cggtcatgtc gagcaggcga ctttctggac gggtttgcgc
    11761 ccgatgacgc cagacggtac gccggttgtc gggagtacac gctttaaaaa tctgtggctg
    11821 aataccggtc acggcacgct cggctggacg atggcttgcg gttccggtca gttgttaagc
    11881 gatctgctct ctggtcgcac gccagcgatc ccatatgagg atctaagcgt agcgcgctac
    11941 agccgtggat ttacgccatc acgtccgggc catttacatg gcgcacacag ctaaggaaac
    12001 gagatgaccc gtccgataca ggccagcctc gatctgcagg cattaaaaca gaatctgtcc
    12061 attgtccgcc aggccgcgcc gcacgcgcgc gtctggtcgg tggtaaaagc gaacgcttac
    12121 gggcatggta ttgagcgtat ctggagcgcg ctcggggcca ccgatggctt tgcattactt
    12181 aacctggaag aggcaataac gttacgtgag cgcggctgga aggggccgat cctgatgctg
    12241 gaaggatttt tccatgctca ggatctggag atttatgacc agcaccgcct gaccacctgc
    12301 gtacacagca actggcagct caaagcactg caaaatgcgc ggctaaaagc accgttggat
    12361 atttatctta aagtgaacag tgggatgaat cggttgggct tccagtccga tcgcgtgctt
    12421 accgtctggc agcagttgcg ggcaatggcg aatgttggcg aaatgaccct gatgtcgcat
    12481 tttgctgaag cggaacatcc tgatggaatt tccggcgcga tggcgcgtat tgagcaggcg
    12541 gcggaggggc tggagtgtcg gcgttcgttg tccaattcgg cggcgactct gtggcacccg
    12601 gaagcgcatt ttgactgggt tcggcctggc attattttgt atggcgcttc gccgtccggt
    12661 cagtggcgtg atatcgccaa taccggatta cgtccggtga tgacgctaag cagtgagatt
    12721 attggtgtcc agacgctaaa agcgggcgag cgtgtgggct acggcggtcg ctatactgcg
    12781 cgcgatgaac agcgaatcgg cattgtcgcc gcaggatacg ccgacggtta tccgcgccat
    12841 gcgcctaccg gtgctcctgt tttagtggac ggcgtgcgca ccatgacggt ggggaccgtc
    12901 tcgatggata tgctggcggt tgatttaacg ccttgcccgc aggcggggat tggtacgccg
    12961 gttgagctgt ggggcaagga gatcaaaatt gatgatgtcg ccgccgctgc cggaacggtg
    13021 ggctatgagt tgatgtgcgc actggcgtta cgcgtcccgg ttgtgacagt gtaacttgtt
    13081 gtaagccgga tcggaggcag cgtcttctgg ttgcgaaaaa atcatccatc cggctggtca
    13141 gcaactgtag ttgttaatgt gacagagcca ttgcccatga tagtgtccat taaaagaatg
    13201 gacactattt ccccggaacc tgaactcgcc gcacaggcgt tctacataaa acgcttacgc
    13261 ttcattgttg actcggagtc gacttcagat aaatcacgct tcacccttga tgcagattcg
    13321 ggttcatgtt cagattgttg attaacaggc tgcgagtcct gttgtctttc cagactcaac
    13381 aggcccaact gttgatccag gagctccagg tcctcctgta ccttcacgcg ctccaggagt
    13441 tccgggtcat ccaggaactc cagatccata aggtcctcca gctcctcgag tccttccagg
    13501 tcaacctagc cttccttgtg caaaaagtcc aaaaggtcct tcaggccctg ctgttttttc
    13561 aggccctcga cggctttgag cagcgtcggt aaattgtctt tgtcccacgg gttaccatta
    13621 cagtcaaaca ccttcaggct ctggggctgc ggcacctgta ccgttttaaa cttgttttct
    13681 ccacagtagt aaattattag tttgggcggc agccgtggtg aagagatcaa atcgttacta
    13741 gaacagtcaa atactttcag gcgagtcaag ttctccagca tctcatcagg tattgagtta
    13801 atattgttgc cggcacagga tagtttatac aggtactggg gcatcgacgg cggtaaaaat
    13861 tgcaagaagt tttcagaaca gttaatcact ctcaggttag tcgacagctc aggcagccag
    13921 ataatactgt tttcatagca cttcagtacc atcagttttg agggctgcac cttcggtaac
    13981 aagatcaaga cgttatctgt acagtcaagc accagcagcc actttggcat caccttcggt
    14041 aacgcggtca acacgttttt actgcagtaa aattcaatca gatgtggagg caaattctca
    14101 gggagtgaag tcaatcccag gttgctaaga ttgagcatac tccgtcttgt aatgagacat
    14161 tccttcattc tgcgcaatgc ttctgcccgg ttttcaatcc ggcttgctgg caggttttct
    14221 tccaggatat tttccttgat ttcttccaga ctttctttca ggtcttcttg gggaatttct
    14281 gactgtttgt tttgctggtt ttcttccagg atttcgtaca ggtttttttg caggttttct
    14341 tgcaaaccat cctgctgtat agcccattcg caccatttgg cgaccatcat gaactcgtta
    14401 tgttgctgat tcagacgagt ccccaacgtg atgatggtat tgttgtccac ctgtatgttc
    14461 actttgcccg cgatactgag gtcaatgatc tgccaggggg catcacccca gctgaatttt
    14521 tgcgggctga gatacttcat gttctgcaaa atttgattct tgagaagagc cgccctggcg
    14581 ggcgtcgtac gcctgggtta acggaagatg ggggaatggc gggggcgaat gggcagatgt
    14641 ctttaaagct agccataatg ctacgtttcc ttgaaataaa tatcctctgg cagcgccgga
    14701 gacgtgaatg tggtcaccgc aaaggagcca caggacgtca tttgcgctga tatcactact
    14761 catcgttgac ttttttcctg gtcagaaatg ccgtgtcggg tctggtacat gctgtattac
    14821 ggcctaggta gctctgacgt attaaagaaa atacgagagt gaacgacccg gaaataatcg
    14881 ttattaagca aaggaataac tataagaaat cgctcatgca tccggtgcta caggcggatg
    14941 aaacgcctat tgtgtgggct atgtcagtgc ggcaggttgc gtgggggaat atgttcaggc
    15001 gatgtcaggc aggggcccct gatggttgac cggtaatgcg gggctggttt ttattacctg
    15061 ttacagtcgg aaaaagccct tacgctgact ttcttgagcc ggtgttttga gttcattagc
    15121 cagagcctgc aatggtttgt catccataat ttaaccttct tctgatgttg ggatgagcat
    15181 atcaaaatca ggaaatgaca agtttattac aggctcaggc tgcgttcagt aaagcatcgc
    15241 atccggcgat agatgctgca ttaaatttaa gattcagctt cctcttcagc cacccgaacg
    15301 ccaatcttca acacttcatt atcttctttc tcggccaccg tccagatcat cccggcaaac
    15361 tctacctggt caccgacaac cggtgccgcg cctagcaact gctggacaat ttcacccagc
    15421 gtttgctgtt tatcacgata ttctcgcccg tcttccaggc catatatcag cgcaacatca
    15481 gcatatttgg cgctggcttc gagaatgaag tcaccaaaga agcgttgatc cagcgcgacc
    15541 ggtggcgact ggctgaacag tttaccgagc gccgggagat cgcgttcccg accaattaca
    15601 cacaatacat cgccttcgcg cagtcgggtg ctgccggtag gatgaagcaa ctggttatca
    15661 cgaaacaggg ctgcaatacg cgtctctttt ggcatatgca aatcgcgcag tgccgcacct
    15721 acacaccatt tatcagcact caattgataa acaaactgct cccacggatt ttccggatga
    15781 atatccaggc caacgcgtga caccggacgt ccgacgggcg gaaccaccac tttggctttt
    15841 ttcgccgccc acgagagtga tgttccctgc aagagcagtg aaaccagaac cacaaagaag
    15901 gcgacattaa agaacagtcg cgcattctcc agccccgcca tcatcgggaa cacggctagg
    15961 atgattggca ccgcgccgcg taatcccacc cagctgataa acacgcgctc acgcagattg
    16021 aaaccgcgga aagggagcaa tccagcaaat accgaaaggg gacgggcgaa gaatatcatc
    16081 catgccgaca aaatgagcgc cggaatggca ataggcagca gatcgcttgg gttaaccaat
    16141 agccccagca ccaggaacat ggcgatttgc gccagccagg cgaggccgtc gaaattttgc
    16201 aggatgccgt agcggttgcg aatcggccga ttacccagca gaaaaccgca cagataaaca
    16261 gccagaatac cgctgccttc cagcgccgtg gttaaagcaa aaattaaaat accgccgctt
    16321 aatgccagca atggatataa tccggcaggc agggcgatgc gattaatcat ttgcagcagt
    16381 aaataaccac cgccaagacc gatgacaatc ccgaggccaa attgctgcag aatatcgacc
    16441 acgaacatcc aactgacact gctttcatgc tgctggatca ttgcaatcag ggtaatcgtc
    16501 agaaagaccg ccattggatc attactgccg gattcaattt ccagcgtcga gccaacacgt
    16561 tcgttaagcc ccttaccacc cagcagagaa aagaccgctg cagcatcggt ggagccgacg
    16621 atagcgccga ttaataagcc ttcaatcaaa tcaagattaa acagccacgc cgccatcatg
    16681 ccggttaaac cagaggtgat aagtacgccc agcgtcgcca gcgacagcgc cggtcctaac
    16741 gccacacgaa aggaactggc ctgagtgcgc atcccgccgt cgagcaaaat aatcgccagc
    16801 gccaggttac ttaccatgta ggcgaagggg taattatcaa acgggatacc gccgacgcca
    16861 tcgactcccg ccagcatgcc gatcgccaga aaaataacca gaataggaat gccaagacgg
    16921 gaagaaaatg aactaagtaa tatactgctg gtgacaagga tggatcctaa gatgaaaagg
    16981 ctaattattg ttgtggcatc caatggtcgg ttactcctga ttacgctgtc tcttatataa
    17041 accctaccat attagcggca gagacagcgt tttacttagc cctgaagcgt gattttttta
    17101 cattttaaga acaggatgac cgctaatcgt caactgagcg ccttcccggg tgttattcag
    17161 aatggcatgt gcgcccaacg gcagcgtaac cgtgcgttgt tcatgaccaa aatcgaggcc
    17221 ggtaatgagt ggaatcgaca ggcgggaacg caaaaacgcg tacactgact ccaggttgta
    17281 acctgcgtca taatcattgg gcgtgctgcc gctaaagcta ccgagaataa tcgccttctg
    17341 acgtggcaaa attcccgcat gatagagctg caacagcata cgttcgacgc ggaaagggtg
    17401 cacgttaata tcttccagca ccagaatacc gttctcaatt tttggcatcc acggtgtacc
    17461 aatcagtgaa atcagcatcg caagattgcc tccccacaac gtgccttcgg tctgacatgt
    17521 cgggccttcc ccttgccatt caatggtgaa ggtttcattg cgtaacgcca gccagaaatg
    17581 gtgctcggta aaggcgttca gttcatccgc accaaagttt gctaccagca tggggccgct
    17641 aaaggtgatg acattgcctt gcgccagaag accgcactga atggcggtaa aatcgctatg
    17701 tccgcaaatg agtaacgggt catgttgttg gcgagccacc agtgcctgcc agtcaatatc
    17761 tgccagtaaa cgactggcac cgtaaccgcc gcgtacagcc agcacgatgg tgttgggggt
    17821 agtcagtctg gcaagggaat tgagatcttc cagacgctcc gtttccgtac ccgcaaaacg
    17881 ctcacaacga cgggcaataa cctcgacgtt attgacttga tgccccgcgt cggtaaggcg
    17941 ctggatacca cgcagcgcgg cgtgctgttt aatgctgtaa cccgatgggg caattaagtg
    18001 aaacagagac atggcaattc cttgctgaca acagaaacga aatgtatatc atgccgctta
    18061 ggtgtgccgt tgtcacctca acggcgattc caggctataa ggatagaaga agtgaaattg
    18121 agatggtttg cctttttgat tgtgttatta gcgggttgtt catcaaagca tgactatacg
    18181 aacccgccgt ggaacgcgaa agttccggtg caacgtgcga tgcagtggat gccaataagc
    18241 cagaaagccg gtgcagcctg gggcgtcgat ccacaattga tcacggcgat tatcgctatc
    18301 gaatcgggtg gtaatccgaa cgcggtgagt aaatcgaatg ccattggttt gatgcagtta
    18361 aaagcttcaa cctccggacg tgatgtttat cgccgtatgg gctggagtgg tgagccgacg
    18421 accagcgagc tgaaaaatcc agagcgtaat atttcaatgg gggcggctta cctgaatatt
    18481 ctggaaaccg gcccgctggc aggcattgaa gatccgaagg tactgcaata tgcgctggtg
    18541 gtgtcatacg ctaacggggc aggtgcgctg ctacggactt tctcgtcaga tcggaaaaag
    18601 gcgatcagca aaatcaacga tttggatgct gacgagttcc tcgatcacgt agcgcgaaat
    18661 catcctgcgc cgcaggctcc gcgctatatc tacaaacttg agcaggcact ggacgcgatg
    18721 taaatcagtc gcgcactttg tccgcttttt cccgggcttc tcgctcgaga gagaaaataa
    18781 tccgctgtaa ttgccgctcc accgtcgggc tgacgttaag aaaacggaag ctcagacggg
    18841 gagtggtgat ggtttcattc ttgccatcaa tcactttgcg ctcgctggtg gagattaact
    18901 gggcgtcaaa gtgaaaaaca ccccattgcc ccatgttgac ttcaatttga gcgaagcgca
    18961 taccttcttg taattcggca ggctttgctg tttccagtaa tgcgcccatg ccgcctaacg
    19021 acaaatcata caagcggaaa cgtaacgtac tgttatccgc cagtttggtc tggcaaaaat
    19081 aaggcggatg gagtggggcg gagatgcgga aatatcggcg tcgttgtaca aaccataagg
    19141 tgggaggcgg tacggtaata aatgccggaa gctgcaagta ttcactctgc tgtagttgtt
    19201 caacagtaaa ctcgactttc gcaccctgag tttcggcggt aatggtaatg tgctgtgcct
    19261 ttagcacggc gatgttgtct tcggcttgac tgccgaaatc cagcaccagt ttatccgggg
    19321 ttattgccag tattttgctg atcagctgcc cgccattcca actgagacgc aaaggaattg
    19381 cggctttgtg caaatcgcgt aacacgccca ggacggctaa cggattttgt ttcaggaact
    19441 gctcatggta atgactcacg cggaaaaact cctgactgac aaactgtctt tggtttatcg
    19501 gtcatagtta acaaaactta atacaaatgc gtgaatattt tttacatgtt gttcttaaat
    19561 cagccgacaa acgcccagca tcgatcccgc ccttgcctat acttagagcg ttgacgtaag
    19621 catttcttgc gaccgattca tcgaaacgag ggcctgaaca tgggaattat tgcctggatt
    19681 atttttggcc tgatagccgg cattatcggc aagctaatca tgccggggcg tgatggtggt
    19741 ggatttttcc tgacctgtat tctcgggata gtcggtgcgg tggtcggcgg ctggctggcg
    19801 accatgtttg gcattgacgg ctccatcagc ggttttaatt tgcacagctt cctggtggcg
    19861 gtggtgggcg ctatcctggt gctgggcgta ttccgcctcc tgcgaagaga ataagatttt
    19921 cataaggcgg atagcgatac agatgccgct atccgctttc acatcagaac gtatactcga
    19981 cacctgccca gaaattgcga ccttcttcaa taaacccttc actgtaggca tattgggtat
    20041 caaacaggtt attgaccgac gcattgacgc tgaagccatg acctaaggtg taatcggctc
    20101 gaatgtgggt caccgcaaaa ccggcggctt tttgtgaacc gtcactgttg ctgtagctgg
    20161 aggaacgcgc ctcttccgac agcgttacgc ttaacggctc ccatggtttg agtgtcatcc
    20221 atgcggtcat tgtctgcgtt ggaagatcgg ttatcttgcc gatgtctttg cgtttggcgt
    20281 cagcgtggat cagagcgtag ctcaatccta catccagtat attgctgatt ttccccttaa
    20341 taccggcatc cagaccgctg taatccaccg tgccgctgtt ctgattttga atggtatcgg
    20401 cattgatatt gtgcgagagg atggcatcac tcacccggtt atagtaaacg ctgacctcaa
    20461 atccccagtc gcgcgtgaag gcaccattcc aggttaaatc caccccgcga gcgcgttccg
    20521 gcttgagctg cgggttaaca atcgctatct ggttatacgc aggtttggat gtggtatagc
    20581 gttctttcag cgtcggaaag cgtgtgcggt catagtacga aagcgccaga gtgtcttcat
    20641 tggcaaagtg gtatttcccc atcacctgcc agttaaaagc tgactgattg ttgtcgtcat
    20701 aatgggtgat gctgccatct ttctcgtgtt ttttcgcttc tacgctatcg cgccagtcat
    20761 agctgattcc cgccacgaca tcgacattat cagcagcagc ccattgatat tcactggcga
    20821 gcgaccaggt acgatcttca tagcgatcgt aagcggcgtg cggcgcacct ttttcccggt
    20881 gtacgtcatc tttccagtta acggcaaacg acagcagatc gttttcacgc acatcggctg
    20941 ccagttgtaa tccggcaccg tcgctgtaat cggaataatg gctgtagctg ccttttttat
    21001 ttttcaaatc agccagcgag ttgtacatca tcagcgtatt ttcaaaggtg tcgcgataca
    21061 gccgactttt cagggtaaaa cggtcgttta gttgggtcgc tccctgataa taaaaacttt
    21121 ctttgtcata ctctggccac tgccagtagc gtgatttttg accacaattt ccgctgtatg
    21181 gcgggttatc tttttcacca tcctgcttaa tgtaagtcag cgtgtattca tcgttttcgc
    21241 gcggtgtaaa accgagcttc acaatgccgc gtttatcatc agccgatgaa ttaatcatct
    21301 tgccgtgttt gcctgcaata tcattattta caccatgcgg caggccgaga aaatcctgct
    21361 ttagctggct accgctgact tgcaaatacc caagatcgct gctggcggca aatgaagcat
    21421 gcatatcgta ggcattgtcc tgactacggc tccatccctg gcgatatccc agacttgctt
    21481 cttcagggtg gtgatattaa tggctccgcc catctgatta ggcccatgaa gcagcgacga
    21541 ataccctttg gaaacttcga ctgcccccag attgttggtc agaatccgcg ccagatcgag
    21601 gttgccgtca taggggacat aaatgggcac accgtcgaaa tagaccggca cctgccgact
    21661 atcaaagcca cgaactttta cctgttcttc gttgcggctg cctgactttt gcagcaccac
    21721 gccggggacg acacttaatg cctgagcgac attctgttta tccaaggcct tcatggtggg
    21781 ttgatcgaga acggtggtcg tcgtcgatga taccggactg gaccatacgg tcagcgtgtc
    21841 gctctcctga gccgctacgg cctgctgggt aaaggcaagt gataatgctg tgcagagata
    21901 acgttttttg agtctcatag aataaattcc gtttaacaaa gaattaaata gaggctgtgg
    21961 gttgcagact gactttaatg tcagctggtg ccgcgtaata aggcgcggag gtaataaaaa
    22021 ggcgaatgcc gcagtcgagg taatttttga gtgtcgtcag attaattccg ccggtcagag
    22081 ccagcgtgca gtgaggagcc agcgacggcg ctatttgcgc aatttcagtt gcctgttgcg
    22141 gactaaattt gtcgagctga agtacgtctg gttgtgcgcg taacgcggcg atagcctctt
    22201 tcggcgtgtc ggcttcaaca acaattttct tctcgggtgc gtggcgacgt aactgagcga
    22261 ttgcgcctga ccagccctga ttgtcatgaa gaaaatggcg gtggttggca aacaataata
    22321 tggtttccgc acatccggca cgatgaatta gtcctccggc agccagaatt gcctgcgaag
    22381 ccagtaagcg ggtgcccgga attgcttttc gggtgcaggc gatattgccg tcaggataac
    22441 gttcacgaag caatgccagc atttgagcga gataatcaga aacaccgcaa ctccactcca
    22501 gcacattctg gactgccttc catccctgat gaagtgctgc ggcattaccc tgcgcacgga
    22561 ttagccgttg acccgcgttc gcttgtgaac cgtcgctgac cgcgtcatca atagttaatc
    22621 ccagcgtagt taacatctta cacgcgacag aaacgccgct gacgcagccg ccctgacggt
    22681 gaaaaaacgc tatatagcca ggctggtgtc caatatttaa tgctcgcgtg gtcaggtcac
    22741 ctccctggat atcttccagc agtaacgcat cgatttgtgc ctgagaaagg aaaatcatca
    22801 cagtgcctcc tgcggtacgc tatcccacca gatgagcgcc cagtcgcggc tggcaggggc
    22861 gagggcacga gcatcttgtt gttgatacca gtgatataac cgctcacgct catcgtcgtt
    22921 aagcgcgcca agactccagc tgacgttctg ctcaaaacgt tcccaggtgc tgttatcctg
    22981 ttggcaattt tgtccacgga taaaatcaac gtgggcataa attcccatct gatacaggac
    23041 gttgagcgca aagatatagt tgggtaattc gatcacttcc cgaccagccg cccgctgaat
    23101 ggctggagag acgaacgagg tgctgaccag atgagttgta tagacgcgaa gccgtgcctg
    23161 attattcagt ttgctcattg catggcgcat atcagccacc agagtggagc gggaagccac
    23221 ggcaatatcg cagcgtggca ggtcactcca gtcctcttcc caggcgcgct ggatccagtg
    23281 gacattgtca gccttcagtg cggcggcgcg gcgggcggca acgttcagca tcccctgact
    23341 gtaatcaacg ccataaaccg tagtgagttt gtcagccagc gccagcgata cggtgcctgg
    23401 tccgcagccc atatcgaata aggtctgagc gccttgcagg tcaattttag cgatcagttg
    23461 ttgcaggtag ctgtccgttg gcgaggcgca attttccgcc attttctccg cccgttgatc
    23521 ccagtgatcg ggcgtttttt ctgtgcggtg agccagtttg agttgctgca gataaaggtc
    23581 tgcaaaatca atatcatcaa tgagcatgat tgttccttag cggctaattg cagagaggtg
    23641 atgatgaatt tggtcggcag aaacccgata cagggccgcc agtttgttcg ttgttaactg
    23701 ttcggtcggt aagccttgtg ttactcgtcc atctggctct acttggataa tgctgtcggc
    23761 aatggcgttg gcatggagcg tatggtgagt cgacattaac attgtcatgc cgttattttt
    23821 gagctgcgcc agcgtgtcca gaagttggat ctgatgaccg aaatcgaggc tggaggcggg
    23881 ctcatccagt aacaacaagc gcggttgttg gacgagtgca cgggcgatta aagccagctg
    23941 acgttcaccg ccgctaagcg tattccagcg tcgtgtcgcc agatgcaaaa tgcccagctt
    24001 ttccagttgc tccgtggctt tcaggcgttc ttgcttaccg ggaacggaaa aagcgcctat
    24061 ggtgggggcc aggcccatca acaccatatc cagcacactg aaagcaaaaa taccgtcgtg
    24121 cgcctgcggc acccaggcaa tagcctgtgc gcgctggcga tggctgagtg tctgcacaga
    24181 gaggctatcc agtaggatct cgccttttaa taacggtata acgccaagaa tggagcgcat
    24241 gagtgtggtt ttgccgcaac cattcgcgcc aagcagacaa caaattgttc cttgcgacag
    24301 ggtgaaggac acgtcgcgta gcactggatg gcgggagtaa cccagagacg catgacgaac
    24361 ggcaagcaaa gtcactggcg acctccgcgt aacaacaaaa gcaggaaaaa cggtgcgcca
    24421 acaaaagcgg tcagaatgcc aagtgggatt tctgttgttc caatgctgcg cgccagcgta
    24481 tcggtaagca ggagcagaat agcgccagta cacattgcca taggcaacag ctgctgatgg
    24541 ttatgaccgg tcaacaagcg ggcaatgtgc ggaacgacca gaccgatcca accaattatg
    24601 cccgctattg ctaccgtgct ggcggtgatt agtgtggcgc agacaatcaa tccaaagcgc
    24661 agacgcgtca cgttcagtcc cagcgaacgg gcttcgtcgt cggaaagcgt gagtaaattc
    24721 atgcgccagc ggagaaaaaa caggggcaag gagcctatga gaatgatcgg tgcagcatag
    24781 cagagatcgc gtaaggtcac ggtggacagt ccccccagca accagaaggt aattgacggt
    24841 aactgggtgt aaggatcggc cagcgtttta attagcgata tccccgctcc gcacaacgtt
    24901 cctaacgcaa tgcctaccag caccagggtt aagatcggat cgtggcgggt cacccgacgc
    24961 gtaatcaacc agacgcccgc gaccaccatc agcccgccgc aaaatgcgta cagctggatg
    25021 taaacaatgg gtaatcccca caggattgcc gcgcaggcac caagtccggc accggcggag
    25081 acgcctaaaa tatccggtga aactaaggga ttacgcaaca ttccctgata ggtggttccg
    25141 gctcctgcca gcgctgcgcc cagtaacagc gcagcaagaa tacgcggcac gcggatttgc
    25201 cagaagacaa tttgttcctg cacagactca ttttcttgac gaagaattag cgccagcagt
    25261 tgctgcatat cgagatgata agccccactt acggcggcaa aagtgatact gatagctgcc
    25321 agcagcaaaa tacatccgtt tacgattctc attagctcgc caccaatttt tgatagtcgg
    25381 catcactgag tgaacaatgc cagaacagtt gggcgtaatg ctgcatgtca cttttaaact
    25441 ggcgattgat ggcgggatcc agccaggcgt gaagtctgcg taatcccaga agacggttga
    25501 ttcccggcgg ggcatcgagc cagccaaagg gcaagccact taaaaataag atgcgttgtt
    25561 ccgcaacggc tttcacgccc tgccagagag gatcacgacg aataaaatct gcagtaacgg
    25621 cctcctgaac cagaataata tccggctgcc agcgcaggag attttccatg gaaacctgtg
    25681 tcaggccgtg gcgatcggct atttgcgcga cgttgtgcaa acctaataat tccgccgcct
    25741 cggtatgcaa cgaaccctgt aagcccgttt ccagccctcg gggaccgcgc gcagcataaa
    25801 agcggaggtt agcggcgggt gaggtggcga atgcctgagc ttcaccaacg aagtggctgg
    25861 cgagattggc ttgttctgcg gcgcggtgct cttcgcctaa cgttttgcca agcgttgtta
    25921 actgttctgc tgattgcgcc agttttccgt taagcaataa ccaggggatt tgtgtctgtt
    25981 cgctaacctg ccgtgcctgg gagatcaagg tttcatccgt attgccgcaa tcaacgacca
    26041 aatcgggatg taacgccata agcccttcca gcgatagtgt gctggcgcgc ccggcgagtc
    26101 gtcccagcct gggtaactgg cgaatgtgct ctggcaatgg aattaatgcc tgacgggcaa
    26161 aatcaaaaga ggaaaaaccg accatttttt ctggcgcgac agccagcaag agcaaatcgg
    26221 cgggcgcgcc tgcgctgacg atgcgtgtaa tgttttgggc ttcgggaaga gattgcgaag
    26281 caaaacgatt aactgtttgt gaaaaggacg gtagagactg caggagcagg gtagatgcaa
    26341 gtgcctgcgc aaacgtccgt ctggttatgg gcatggtgaa catcctgaaa agtaaaatcg
    26401 atatatactt tcatatatat cgataaaaat catgttcaat atcaaagttt agctattgaa
    26461 aacatgaaga tgtgacgact tccgcaagcc tgttgaccct ctctatcatt aaaaatgtat
    26521 ttatcaggtg gctaatatta tttaaatcat ttaaggctaa attatttaaa tatgaatgac
    26581 attattgttt ttttatcgtc cacggttacg cgacgttgaa gttacttgtt ttataatgag
    26641 cgatttttga tatctcgccg agaaaattat cagcattctc tcgtttcagt gtcgtgaata
    26701 tactgttaac ggaggtgaaa agaatgctgg gggttataca aaacctatgt tgttgtcgtc
    26761 cggattcacc gcttgctgat agtaagcagc gtaaccagaa gttaccgcaa caagactttg
    26821 aggattttaa gaacgacgtt attaatgtca ttaacaacaa tgaagatatt tattttctta
    26881 gagagagtaa tttactgaat gcgtttattc ggacgtgcaa aaaaagagat gataacagtt
    26941 acggaaatat gatatttacg ctttattttg ataacgatat ctatgggaac agaattatca
    27001 aagcatcgct gaacggcgga gagttgcagt tattaagcac agggtgtagc aatgataaca
    27061 ttctcc
//
LOCUS       DAEBXN010000053        27045 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.053, whole genome shotgun sequence.
ACCESSION   DAEBXN010000053 DAEBXN010000000
VERSION     DAEBXN010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27045
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.053"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(<1..176)
                     /locus_tag="KJL99_002277"
     CDS             complement(<1..176)
                     /locus_tag="KJL99_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HBD4436987.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQDR"
     gene            complement(260..1144)
                     /gene="yhdJ"
                     /locus_tag="KJL99_002278"
     CDS             complement(260..1144)
                     /gene="yhdJ"
                     /locus_tag="KJL99_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HBD4436988.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDAFAELKKLPTESVDLLFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPKALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(1230..1526)
                     /gene="fis"
                     /locus_tag="KJL99_002279"
     CDS             complement(1230..1526)
                     /gene="fis"
                     /locus_tag="KJL99_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HBD4436989.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(1552..2517)
                     /gene="dusB"
                     /locus_tag="KJL99_002280"
     CDS             complement(1552..2517)
                     /gene="dusB"
                     /locus_tag="KJL99_002280"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HBD4436990.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(2846..3727)
                     /gene="prmA"
                     /locus_tag="KJL99_002281"
     CDS             complement(2846..3727)
                     /gene="prmA"
                     /locus_tag="KJL99_002281"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HBD4436991.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(3739..5190)
                     /gene="panF"
                     /locus_tag="KJL99_002282"
     CDS             complement(3739..5190)
                     /gene="panF"
                     /locus_tag="KJL99_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HBD4436992.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIIVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5180..5422)
                     /locus_tag="KJL99_002283"
     CDS             complement(5180..5422)
                     /locus_tag="KJL99_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HBD4436993.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVDAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(5531..6880)
                     /gene="accC"
                     /locus_tag="KJL99_002284"
     CDS             complement(5531..6880)
                     /gene="accC"
                     /locus_tag="KJL99_002284"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HBD4436994.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSTDRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(6891..7361)
                     /gene="accB"
                     /locus_tag="KJL99_002285"
                     /gene_synonym="fabE"
     CDS             complement(6891..7361)
                     /gene="accB"
                     /locus_tag="KJL99_002285"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HBD4436995.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(8339..9313)
                     /locus_tag="KJL99_002286"
     CDS             complement(8339..9313)
                     /locus_tag="KJL99_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBD4436996.1"
                     /translation="MQALLLEQQDGKTLASVQTLDERRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASGILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            9465..11405
                     /gene="csrD"
                     /locus_tag="KJL99_002287"
     CDS             9465..11405
                     /gene="csrD"
                     /locus_tag="KJL99_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HBD4436997.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQILIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            11710..12753
                     /gene="mreB"
                     /locus_tag="KJL99_002288"
     CDS             11710..12753
                     /gene="mreB"
                     /locus_tag="KJL99_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HBD4436998.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            12819..13922
                     /gene="mreC"
                     /locus_tag="KJL99_002289"
     CDS             12819..13922
                     /gene="mreC"
                     /locus_tag="KJL99_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HBD4436999.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGISQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PVAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            13922..14410
                     /gene="mreD"
                     /locus_tag="KJL99_002290"
     CDS             13922..14410
                     /gene="mreD"
                     /locus_tag="KJL99_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HBD4437000.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSMLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            14419..15012
                     /gene="yhdE"
                     /locus_tag="KJL99_002291"
     CDS             14419..15012
                     /gene="yhdE"
                     /locus_tag="KJL99_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HBD4437001.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADIIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15002..16471
                     /gene="rng"
                     /locus_tag="KJL99_002292"
     CDS             15002..16471
                     /gene="rng"
                     /locus_tag="KJL99_002292"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HBD4437002.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            16539..20339
                     /gene="yhdP"
                     /locus_tag="KJL99_002293"
     CDS             16539..20339
                     /gene="yhdP"
                     /locus_tag="KJL99_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HBD4437003.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLNAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSILKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            20495..21940
                     /gene="tldD"
                     /locus_tag="KJL99_002294"
     CDS             20495..21940
                     /gene="tldD"
                     /locus_tag="KJL99_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HBD4437004.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22068..22997)
                     /gene="aaeR"
                     /locus_tag="KJL99_002295"
     CDS             complement(22068..22997)
                     /gene="aaeR"
                     /locus_tag="KJL99_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HBD4437005.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSANLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            23180..23383
                     /locus_tag="KJL99_002296"
     CDS             23180..23383
                     /locus_tag="KJL99_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HBD4437006.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            23391..24323
                     /gene="aaeA"
                     /locus_tag="KJL99_002297"
     CDS             23391..24323
                     /gene="aaeA"
                     /locus_tag="KJL99_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HBD4437007.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSDLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            24329..26296
                     /gene="aaeB"
                     /locus_tag="KJL99_002298"
     CDS             24329..26296
                     /gene="aaeB"
                     /locus_tag="KJL99_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HBD4437008.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            26388..26660
                     /locus_tag="KJL99_002299"
     CDS             26388..26660
                     /locus_tag="KJL99_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HBD4437009.1"
                     /translation="MNIYAFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(26716..26979)
                     /gene="yhcN"
                     /locus_tag="KJL99_002300"
     CDS             complement(26716..26979)
                     /gene="yhcN"
                     /locus_tag="KJL99_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HBD4437010.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
BASE COUNT         6443 a         6786 c         7229 g         6587 t
ORIGIN      
        1 attctgtcct gaaaattcag ttcgtcacag ttaaccagtt ttagtggatt aactgagttc
       61 atattacggc atttgtctgt ttctgttgtc agcagttcaa tccactgcat cagtaaagta
      121 tcaaacagaa aaacgaagac agcgaaaacg acgagccacc aatacttgcg aatcattacc
      181 tgagcctgtg catgtttaac caagtctggc aggaaatata cacgaaatga ataatctgaa
      241 aactaggctt acacttcctt tactttgcaa tgagatcggg gtcaacttcg cttaaccggc
      301 ttcgttttga caggttgccg gtctttcttt ttttcacttt cgccagttct tccgcggagt
      361 aatgcgacgc gacatccagc cgtcgaagcc ccattttgat gtactcgctg ttgatctcaa
      421 taccaatgaa ttttcgtccg ctggcgatgg ctacggcacc ggtagtaaag ctaccggcaa
      481 acgggtcgag aacgatatcg cctgggttgg aagaggcgag aataatgcgt ttcagtaagg
      541 ctttcggttt ttgcgtcggg tggttctcat attcatccat caaataacgc acgcgcggaa
      601 aatcccagac gttacccggt actttttgat gattgtatgg ctgtggagga tttttgcgat
      661 aatcgatcaa cgcgcgctgc gatccggttt tggcttctac cagaatagca tcaccgttga
      721 atgtgtagtt ctttgcgtct ttcaccatca tcaggatggg ttcgtacatg gagccgtagt
      781 gttttttcgc ctgtactcca gaactgtcat atgaccagac gatgcgactt ttgatggtaa
      841 aaagcttgcg gcactggaga tcgataaagg gcatgttttc cgtactgttc ataatgtaca
      901 tgctgccctg ctttttcaga acgcggtggc actctgcaat cacttcaaac agccagtcga
      961 taaacagatc ttctttccag gcttcgatca gaccatcaaa atttttaccg atgttatacg
     1021 gtgggtcggc aaagagcaga tcgacgcttt cagtaggtag ctttttaagt tcggcaaagg
     1081 catcaccgtg aataatggtc ttagcttcat taccaaaccg ggtcggttca catcctgttc
     1141 tcatggtcac tccctttgtg acacctataa aaaaggcgct tccccatgcc gagtagcgcc
     1201 tttttaatca agcatttagc taacctgaat tagttcatgc cgtatttttt caatttttta
     1261 cgcagcgtac cacggttgat gcccatcatc agcgcagcac gggtctggtt accacgggtg
     1321 tattgcatca ccatgtccaa caggggctgt tctacttcag ccagtaccag ctcatagagg
     1381 tcattcacat cctgaccatt cagttgagca aaatagttct tcagtgcctg tttaaccgag
     1441 tcacgcaggg gtttttgggt tacctgatcc tgagagttaa cggtagaaac ggtcagtacg
     1501 tcagaattta cgcgttgttc gaacatagtt ctgtcagctc tttatttctg tttacgcaaa
     1561 attttcgaag tatgcctcca acgcctccag ctgttcgctg gcatcctcaa tggcgttgaa
     1621 tgtgcgccga aactggtcat ttggagcgtg ttcctggaga taccaggaaa cgtgtttacg
     1681 tgcaattcgg tacccttttg ccggaccata aaagtcatgc agttcccgaa cgtgcgcgca
     1741 aagcaagcgc ttaacctctg ccaaaggcag tgggggcagc aactccccag tgtccagata
     1801 atgctggatt tcccgaaaga tccagggtct tccctgagct gcgcggccta tcatcagggc
     1861 atccgcccct gtatagtcga gcacagctct ggctttaagc gggtcagtaa tgtcgccatt
     1921 cgcgataacc ggaatggaaa ctttctgctt aactgcccga atactgtcgt actcagcttc
     1981 tccattgaac aaacaggcgc gtgtacggcc atgaatggtc agagcctgaa tgccacagtc
     2041 ttcagccagt tgggcaatct cttcgcagtt acggtgttcc ggtgcccagc cggtgcgaat
     2101 cttcagggta acaggaacgt ccactgcatt gacgacctcg gtaaggatcg atttaacgac
     2161 atccgggtac tgcaagaggg ctgaacctgc gagcttgcga ttcacttttt tagccgggca
     2221 acccatattg atatcaataa tctgggcacc gctttccacg ttaatacgtg ctgcatctgc
     2281 catttctttc ggagcgctac cagcaatttg cacggtgcga ataccgggtt catcaatgtg
     2341 caccatccgt aaacgagatt tgtcgctttc ccaaacctgt gggttagaag acatcatctc
     2401 ggatactgtc aatccggctc ccatctcgta gcacaacgtc cgaaaaggtc tgtctgtaat
     2461 gccagccatg ggcgctgcga tcaggcgatt tctgagctga tattgtccga tgcgcatgag
     2521 ttaagaaatg accatactgt gactgcaagg cggcgtatat tacgcatttt ttgcacgaga
     2581 tgaaaggcca aactttgacc aatcctctga gatggatcaa agaattgcat ttaaaatgag
     2641 cgtggtgcga taattactca taaaaatcat catattagaa aatagtgact aaaatttaca
     2701 ctcaaagaaa tttgagtaag ttctcaattt ttctttatga atgaaaattt tggcacgcaa
     2761 atttgcgtaa ataatcggca aatttacgtg ccttttgtga gcttgctcgc acttcgcccc
     2821 gcgtcaccct acggcgatgc gaaggtcaat tcttacgacc ggtaatacgg caccactctt
     2881 ctttttccac gaccgggtcc agtgcgaagc tatcggcata agcttcacaa acgctctctg
     2941 cctggcttgc cagaataccg gaaaggccca gcaaaccgcc tgaaactggc aggacgctga
     3001 ttaacggtgc cagttcacgt aatgggcctg caaggatgtt agcgaccacc acgtcggctt
     3061 tcatttcttc tggctgatct ttcggtaagt agagttccag acggtcagaa acgccattac
     3121 gttcggcgtt atcgcggctg gcctgaatcg cctgcggatc gatatcaata ccaatggctt
     3181 ttgctgcacc cagtttcagc gccgcgatcg ccagaatgcc ggaaccacag ccaaagtcga
     3241 tgactgtttt accggttaaa tcgaggctgt cgagccattg caggcacaga gaggtggttg
     3301 gatgggtacc cgtaccaaac gccagccctg gatctaacat cacgttgacg gcgttttcgt
     3361 ccggcacatc acgccagcta gggcagatcc acagtcgttc accaaagcgc atcgggtgga
     3421 aattatccat ccattcgcgc tcccagtctt tatcttctag ttgttcgatt ttatgcgcga
     3481 agcctgcgcc gagcagcgga tggttttcca gaatcgccac cacgtcgttc atatcggttt
     3541 cagcgtcgaa cagaccaatc acatcggtgt cgccccacag gcgcgtttcg cccggcagcg
     3601 gctcaaatac tggcgtatcg tgggtatcct gaaaagtgat agaaacggca cccgcttcca
     3661 tcagcgcatc gctaagatct tccgcgttcg cgccggtggt gttcagtttc agttggatcc
     3721 aaggcatggc aaaactcttt atttatcagt agtcaaaacg gtagcttgcg ggacggatgt
     3781 accgaaacgg tttccgacca ggaaagccag caaacttagt agtaacgagg gcacgatagg
     3841 gtggaagccc aggtactgaa tattcagcgt cgcgagtacg gcatacagca cgccgccaac
     3901 gatcatcgca cttagcgcgc ctttggcgtt ggcgcgttcc cagtaaagac ccagcaccag
     3961 cggccacagg aaaacggctt ccagcccacc gaaggccagc aaattcagcc agatgatcat
     4021 ttctggcggc ttccaggcgg caagcagcag caacgcgccg agaactaacg taattaccgc
     4081 cgacatccgc ttcagacgcg tctcgttttg catttgatcc ggacggatat tcagatagag
     4141 atctttaatg atcgtagcgg aactttgcag cagttgggcg ttaattgtcg acatgatcgc
     4201 agccataggt gcagccagaa agatcccggc agcaaaaggt ggcagcactt ttaccattaa
     4261 cgtcgggatc accagatctg gtacggtgag atcggggatc actgcacgac ctaacgctcc
     4321 ggccaggtgc ataccgaaca tcaggattgc gactataatc gtaccgatga tgatcccccg
     4381 gtgtactgct ttgctgtctt tataagagat acagcgcacc gcagtatgcg gcaggccaat
     4441 cacgccaaag cacaccagta cccagaacga cgtcataaag gcaggcgaca gaatatcgtc
     4501 agcgccttgt ggcgtaacca gttgcggatc gatggtttgc aaggtctgta ctgcgttgct
     4561 taagccgcca gcggcatgta ccacgccaat aagcagcaca acggtgccaa tcagcatcac
     4621 aagcccttgc atggtgtcgt tcagcacgct ggcgcgaaag ccaccaaagg cggtatataa
     4681 cgcaatgctg ataccaaaaa tcagcagccc ggtttcataa ggaatacccg ccgcggtttc
     4741 cagcaggcgc gcaccgccga taaactgcac ggtcatcgca ccaacgaacg caaccagcaa
     4801 actcaaactc gccagccaca ccagaagacg actctggtag cgggcaaaca gcatatcgtt
     4861 cagcgtcact gcattgtagc ggcgcgcaag aatcgcaaac ttcttgccga gaataccgag
     4921 tgaaagccag acagcaggaa gctgaatcat cgccagcaat acccagccca gcccgtattt
     4981 ataagcagct cctggcccgc cgataaacga actggcactg atataggtcg cggtgagagt
     5041 catcgccagc acaataccgc ccatagagcg gctgccgagg aaatactcat taaggaaggt
     5101 gccggtgctc cgtttacgca tcgcataaac cgagataccg aacaccacca ccagataggc
     5161 gaccagcggt agaattactt caagctgcat cgtcatcctc cagtgggata tcgcgataga
     5221 taaatttcac catcgcccag cacagtccaa taaacagtag cggcgtcagg atgcaggcca
     5281 tctcaaacca gcgcggaaag ccggtaaaac cgggggcaac gccaggtaag taagcgtcta
     5341 ctaaccaaac tgccagatac aaaagggtca gccccagcgc ccagcgggcc tctttatggg
     5401 cctgaacaaa acgagtgtcc attttttgtc cctgatgggt gaagaaagcg aggattgtac
     5461 cttatggggg ttgtcgatcc ccagtaataa aaaaggccgg aaaatccggc cttttgacgc
     5521 ttttgcagtc ttatttttcc tgaagaccga gttttttctc cagatagtgg atgttagtgc
     5581 caccatgctg gaagttctcg tcattcatga tgcggatctg cagatcaacg ttggttttga
     5641 taccgtcgat gatcagctcc tgcagcgcat tcttcatgcg ggcaatcgcc acgtcacggt
     5701 tttcaccgta gcaaatcagc ttaccgatca ttgagtcata gtacggcggt acggtgtagc
     5761 ccgcgtagat atgagactcc caacgtacgc caaaaccgcc aggtgcgtgg aaacgggtga
     5821 ttttgcctgg gcttggcagg aaggtgttcg gatcttcggc gttgatacga cattccaccg
     5881 catggccgcg aacgtgaact tcttcttgct tgatcgacag cggttgaccg gcagcgatac
     5941 gcagctgttc tttgatcagg tcaacgccgg tgatcatttc tgtaaccggg tgttctacct
     6001 gaatacgggt gttcatttcg atgaaataga actcgccgtt ttcgaacagg aactcgaaag
     6061 tacctgcacc gcgatagccg atatcaacac acgctttagc gcaacgttcg ccgatgtagc
     6121 gacgcagttc cggggtaatg cccggtgctg gtgcttcttc gaccactttc tggtggcggc
     6181 gctgcatgga gcagtcacgt tccgccagat agatagcgtt gccctgaccg tcagccagta
     6241 cctgaatctc gacgtggcga ggattctcca ggtatttctc catgtaaacc atatcgttgc
     6301 tgaaagcagc tttcgcttcc gcacgggtca tggagatgga ttgtgccagt tcagcgtcgc
     6361 cgcgcactac gcgcataccg cgaccgccgc cgccgccgga ggctttgata atcaccggat
     6421 aaccaatgcg tttagcaatg gcgcggtttt tatccatatc gtcgcccagc gggccgtcag
     6481 aaccaggtac gcaaggaacg cccgcttttt tcatcgccgc gattgcggat actttgtcgc
     6541 ccatcaggcg aatggtttct gctttcgggc cgatgaagat aaagccggag cgttcaacct
     6601 gctcggcaaa gttggcgttc tcggagagga agccgtaacc cggatggatt gctactgcgc
     6661 cggtgatttc agcggcgctg atgattgccg ggatgttcag ataacttttt actgacggag
     6721 cagggccaat acagaccgtt tcatctgcca gtaatacgtg ttttagatcg cgatccgtgc
     6781 tggagtgcac agcgacagtc ttgatgccca gttctttaca ggcacgaaga atacgcaatg
     6841 caatctcgcc gcggttggca ataacaattt tatccagcat gttcgcctcg ttactcgatg
     6901 acgaccagcg gctcgtcaaa ttctaccggt tgtccacttt cgaccagaat tgctttcacg
     6961 gtaccggatt tgtccgcttc gatctggttc atcattttca tggcttcaac gatgcacagg
     7021 gtatcgccca cgttgacttt ctgacccact tcgatgaacg cttttgcgtc cgggcttggg
     7081 gtgcggtaga aagtaccaac catcggggaa cgtacgatgt gaccactgat ttccgctgct
     7141 gctggcgctt ccatggaagg aacggtcgcc ggagcggctg cgttagattg agctggctgc
     7201 tgcatcattg gtgcagcgta agcttgttgc atcacaggga aacttgcggc aggagctgca
     7261 cggctaatgc gtactgactc ttcgccttca gaaatttcca gttcggagat gcctgattct
     7321 tcaaccagct cgatcagttt tttaatctta cgaatatcca tgagtgggtt ccgtactctt
     7381 tgtttagtgt gattgtgaca ggcgtttcac cgccgtctgt aaagcgtatg ccccgacagc
     7441 tgtatgcata gcgataaatt ccagcaggcc gggacgccgg tctattttgc cccagagtgt
     7501 caatgttaga cttgacggac attgtgcagc gatctgctgc tatcctccgg caaaaaacaa
     7561 aatatacctt cgttgcgcca atgtcacctt ttccgcagcg caaaaactgc gtcagggagg
     7621 acgaggtcgc acattataac gatttcgtag caattggcag ctaaatactg gtcttatcag
     7681 ggaatataat caacagctaa catgtaaata accttcaaca ccgtgtaatt tgcaacaagc
     7741 cgcacaattc acgaaattag cgccatcatc gacggaactt cttataacgt aaggtgaaaa
     7801 gctgaaaagc cagccctgcg aagatgaggg gctgcaatga gataatcttc acagaccaca
     7861 aataatgtag ggatgccagg atcgcgacaa gatagacgac attacgcagc ggttgctggg
     7921 gattacccaa ttttcactgc atcgtctgga tagatgtgaa tgctaaagca cgcaaaatca
     7981 accaactgac aattctcagc attaaatagg ttcgagtggt taatttgtag tcaaataaaa
     8041 tcagattatt caccccatgg taccagaaat cagtaacagg ttaagcgtag tgtcgcccag
     8101 gcaaagcacc ataaacctaa caggcgacgg aggcgtatca ataatggctg tttagaggag
     8161 tatcaatgcg catcaatttg tggtcagtcg aaatcatgac tttccagttt ggatgatgtt
     8221 gtacaacttc ctgaccaagg tgttcgacga aagctgtccg gctaaccgct tttcccgtta
     8281 accgattgaa taatgatgtc aaaatgagtt ccctctctta ttattccttg ctaaatggtt
     8341 agttaacctt caccagcgtg cgaccctgga tctggttatt aatgatggcc tcggcaaagt
     8401 tcggtgcctc tgacagagat atctcttttg ccgcctgggt atagaatgat tccggtaaat
     8461 cggcgaccag tcgctgccag gcttgtgcgc ggcgttctgg tggcgtcatt actgaatcca
     8521 ccccttgcaa acggacatta cgcagaataa atggcatgac cgtggttggc agagtaaaac
     8581 cacccgccag accacaggcc gccacgcagc cgccgtaatt catttgcgcc agcacttttg
     8641 ccagcacttt gtcgccaaca gtgtcaattg ccccagccca gacttgtttt tccagaggac
     8701 gggattcggc aaactcatca cgagggagaa taccgctggc acctaaactt ttcagatatt
     8761 catgggtact ttcgcgaccg gaaacggcaa cgacctgata acccaactta tgcagcagcg
     8821 ccacggcggt actgccgacg ccaccactgg cacccgtcac gacaatctca ccgtcctgcg
     8881 ggcgaacacc ggcatcttcc agcgccatca cacacagcat ggcggtaaaa ccggcagtac
     8941 cgataatcat tgctttacgc gcgtccagcc cttgcggcat ggcaaccagc cagtcacctt
     9001 tcactcgcgc ctgctccgcc agcccacccc agtggttttc accaacgccc cagccagtga
     9061 gtaacacctc ctgaccggca tgaaaacgcg gatcttcgct ggtgcgtaca gttccggcaa
     9121 aatcgatccc aggaatcatc ggaaaattac ggatgatttt tcccttaccg gtaatcgcca
     9181 gcgcatcttt atagttcagg ctcgaccagt gaacatcgac cgtgacatcg ccctccggca
     9241 ggcgacgttc gtccagagtc tgtactgatg cgagagtttt gccgtcctgc tgttctaaaa
     9301 gtaacgcctg cataagtggt cctcatgtgc atgatggatt ggaaaataat ttctgaagac
     9361 tatactcgct aatggaaaag caaagccgat gaagcgcaat aaattgcgag ataaatctga
     9421 tttgctagta tgcccgcttc ctcactatcg gagttaacac aaggatgaga ttaacgacga
     9481 aattttcggc ctttgttacg ctgctcaccg ggttaacaat ttttgtgact ttgctgggct
     9541 gttcgctaag tttctacaac gccattcagt ataagtttag tcatcgcgtt caggcggtgg
     9601 cgacggcgat tgatacccac cttgtgtcga atgacttcag cgcattaagg ccacaaatta
     9661 ccgaattaat ggtgtcagca gatatcgttc gtgtagacct gctgcatggt gataaacagg
     9721 tttataccct ggccagaaat ggtagttatc gtccggttgg caccagcgat ctgtttcgtg
     9781 aactgagcgt tccgttgata aagcatccgg ggatgtcgtt gcgtctggtt tatcaggatc
     9841 cgatgggcaa ctatttccat tcgttgatga ccaccgcgcc gctcacgggg gcgattggct
     9901 ttatcattct tatgctcttt ctggcggtac gctggttaca acggcaactt gccgggcaag
     9961 aattgctgga aacccgggct actcgtatct taaacggtga gcgtggctct aatgtgttgg
    10021 gaaccatcta tgaatggccg cccagaacca gcagtgcgct ggatacgctg cttcgtgaaa
    10081 ttcagaacgc acgcgaacaa cacagccgtc ttgatacgct gatccgctct tatgccgcac
    10141 aggacgtgaa aaccggcctc aataaccgac tctttttcga taatcagtta gcaacgttac
    10201 tggaagatca ggagaaagta ggtacccacg ggatcgtgat gatgattcgt ctgccggatt
    10261 tcaatatgtt gagcgatacc tgggggcaca gccaggttga agaacagttc ttcactctga
    10321 cgaatctgct gtcgacattt atgatgcgct accctggcgc actgctggcg cgttaccacc
    10381 gcagtgattt tgctgcgctg ttaccgcacc ggacgttaaa agaggcagag agcatagcca
    10441 gtcagttaat caaagccgtc gataccttgc cgaacaataa aatgctcgat cgcgacgata
    10501 tgatccacat tggtatctgt gcctggcgta gtggtcagga taccgagcag gtaatggaac
    10561 atgcagagtc tgccacgcgt aatgcgggat tgcagggtgg caatagctgg gctatttatg
    10621 atgactcgtt gcctgaaaaa ggacgcggta atgttcgctg gcgtacgctt atcgagcaaa
    10681 tgctcagtcg cggcggcccg cgcctttatc aaaaaccggc ggttactcgc gaaggtcagg
    10741 ttcatcatcg cgaactcatg tgccgcatct tcgatggtaa tgaagaggtt agctcggcgg
    10801 agtatatgcc gatggtcttg cagtttggct tatcggaaga gtatgaccgt ctgcaaatca
    10861 gccgtcttat tccactattg cgttactggc cagaggaaaa tctggcgatt caggttaccg
    10921 ttgagtcgct gattcgtccg cgttttcagc gttggctgcg cgatacgtta atgcaatgtg
    10981 aaaaatcaca acgaaaacgc ataattattg aacttgcaga ggccgatgta ggtcaacata
    11041 tcagtcgttt acaacctgtt attcgtttag tgaatgcttt aggggtacgg gtagccgtca
    11101 accaggctgg tttgacgctg gtaagcacca gttggatcaa agaacttaat gttgagttac
    11161 tcaagctcca tccggggctg gtcaggaaca ttgagaagcg aacggagaac cagctgctgg
    11221 ttcaaagcct ggtggaagcc tgctccggga ccagcaccca cgtttacgcc accggcgtgc
    11281 gttcgcgaag cgagtggcag atcctgattc agcgcggtgt tacaggcggg caaggggatt
    11341 ttttcgcgtc ctcacagcca cttgatacta acgtgaaaaa atattcacaa agatactcgg
    11401 tttaacctgc cgtttaatcc gttttcacgt agaataacgc gcgctgcgtc tcatgggagt
    11461 gtgcttgtct gctcgccaga ttgttgcagc acatatgcag atgaatgacc ttacgcggtt
    11521 gcaaacaggc gaggaatgct gctgatgcat taagcctttc tggactcagg cagagatttg
    11581 taacaaagga aacgaactgc actaattttc accgtagcag atgatttttg cgccttgtcg
    11641 ctgctgcgtg tggttggtaa agtaagcgga ttttcttttc cgccccagct ttcaggatta
    11701 tcccttagta tgttgaaaaa atttcgtggc atgttttcca atgacttgtc cattgacctg
    11761 ggtactgcga ataccctcat ttatgtaaaa ggacaaggca tcgtattgaa tgagccttcc
    11821 gtggtggcca ttcgtcagga tcgtgccggt tcaccgaaaa gcgtagctgc agtaggtcat
    11881 gacgcgaagc agatgctggg ccgtacgccg ggcaatattg ctgccattcg cccaatgaaa
    11941 gacggcgtta tcgccgactt cttcgtgact gaaaaaatgc tccagcactt catcaaacaa
    12001 gtgcacagca acagctttat gcgtccaagc ccgcgcgttc tggtttgtgt accggttggc
    12061 gcgacccagg ttgaacgccg cgcaattcgt gaatccgcgc agggcgctgg tgcccgtgaa
    12121 gtcttcctga ttgaagaacc gatggctgcc gcaattggtg ctggcctgcc ggtttctgaa
    12181 gcgaccggtt ctatggtggt tgatatcggt ggtggtacca ctgaagttgc tgttatctcc
    12241 ttgaacggcg tggtttactc ctcttccgtg cgcattggtg gtgatcgttt cgacgaagct
    12301 atcatcaact atgtgcgtcg taattacggt tctctgatcg gtgaagccac cgcagaacgt
    12361 atcaagcacg aaatcggttc ggcttacccg ggcgatgaag tccgtgaaat cgaagttcgt
    12421 ggccgtaacc tggcagaagg tgttccacgc ggttttaccc tgaactccaa cgaaatcctc
    12481 gaagcactgc aggaaccgct gaccggtatt gtgagcgcgg taatggttgc actggaacag
    12541 tgcccgccgg aactggcttc cgacatctcc gagcgcggca tggtgctcac cggtggtggc
    12601 gcactgctgc gtaaccttga ccgtttgtta atggaagaaa ccggcattcc agtcgttgtt
    12661 gctgaagacc cgctgacctg tgtggcgcgc ggtggcggca aagcgctgga aatgatcgac
    12721 atgcacggcg gcgacctgtt cagcgaagag taatcggatg caggcagggg aagcgtctgt
    12781 ttaccctgcc tggtctgata cgagaatacg cataacttat gaagccaatt tttagccgtg
    12841 gcccgtcgct acagattcgc cttattctgg cggtgctggt ggcgctcggc attattattg
    12901 ccgacagccg cctggggacg ttcagtcaaa tccgtactta tatggatacc gccgtcagtc
    12961 ctttctactt tgtttccaat gctcctcgtg aattgctgga tggcatatcg cagacgctgg
    13021 cctcgcgtga ccaactagaa cttgaaaacc gggcgttacg tcaggaactg ttgctgaaaa
    13081 acagtgaact gctgatgctt ggacaataca aacaggagaa cgcgcgtctg cgcgagctgc
    13141 tgggttcccc gctgcgtcag gatgagcaga aaatggtgac tcaggttatc tccacggtta
    13201 acgatcctta tagcgatcaa gttgttatcg ataaaggtag cgttaatggc gtttatgaag
    13261 gccagccggt catcagcgac aaaggtgttg ttggtcaggt ggtggccgtc gctaaactga
    13321 ccagtcgcgt gctgctgatt tgtgatgcga cccacgcgct gccaatccag gtgctgcgca
    13381 acgatatccg cgtaattgca gccggtaacg gttgtacgga tgatttgcag cttgagcatc
    13441 tgccagcgaa tacggatatt cgtgttggtg atgtgctggt gacctccggt ctgggcggtc
    13501 gtttcccgga aggctatccg gtcgcggttg tctcttccgt aaaactcgat acccagcgcg
    13561 cttatactgt gatccaggcg cgtccgactg caggactgca acgtttgcgt tatctgctgc
    13621 tgctgtgggg cgcggatcgt aacggcgcta acccgatgac gccggaagag gtgcatcgtg
    13681 ttgctaatga acgtctgatg cagatgatgc cgcaggtatt gccttcgcca gacgcgatgg
    13741 ggccaaagtt acctgaaccg gcaacgggga tcgctcagcc gactccgcag caaccggcga
    13801 caggaaatgc agctactgcg cctgttgcgc cgacacagcc cgctgctaat cgctctccac
    13861 aaagggctac gccgccgcaa agtggtgctc aaccgcctgc gcgtgcgccg ggagggcaat
    13921 agtggcgagc tatcgtagcc agggacgctg ggtaatctgg ctctctttcc tcattgcgct
    13981 gttgctgcaa atcatgccct ggccggataa cctgattgtt ttccggccaa actgggtgtt
    14041 acttatcttg ttgtattgga tcctggcctt gccccatcgc gtaaatgtgg gcacaggttt
    14101 tgtgatgggt gccatactgg atctgatcag cggctcgatg cttggcgtac gcgtattggc
    14161 gatgagcatc attgcttacc tggtggcgct gaaataccag cttttccgca acctcgcatt
    14221 atggcagcag gcgctggtcg tcatgttgct ttcgctggtg gtggatatta ttgttttctg
    14281 ggcagagttt ttagtgatta acgtctcttt cagaccggaa gtgttctgga gtagtgtagt
    14341 caatggggtg ctctggccgt ggattttctt gctgatgcgt aaagtccgtc agcagtttgc
    14401 agtgcaataa aggtttctat gacttctctg tatttagctt ccggttctcc gcgtcgtcag
    14461 gagttacttg cgcaacttgg cgtgaccttt gaacgtattg ttacgggcat tgaggagcag
    14521 cgtcagccgc aggagagcgc gcagcagtat gttgtgcgtc tggcacgcga gaaagcacag
    14581 gcgggtgtcg cgcaaacggc acaggatctc ccggtgctgg gtgcggatat tatcgttatc
    14641 ctgaacggag aagtgctcga gaaaccgcgc gacgcagagc atgcggcgca gatgttgcgc
    14701 aaattatcgg gtcagaccca tcaggtgatg acggcagtgg cgttggccga cagccagcac
    14761 attctcgatt gcctggtggt caccgatgtg actttcagaa cgttaacaga cgaagacatc
    14821 gcgggctatg tcgccagcgg tgaaccgtta gataaagcag gtgcatacgg tattcagggg
    14881 ctgggtggct gttttgtcag gaagataaat ggcagctatc acgccgtagt cggcttaccg
    14941 ctggttgaaa cgtatgaatt attaagtaat tttaacgcac tgcgtgagaa aagggataaa
    15001 catgacggct gaattgttag taaacgtaac gccttcggaa acgcgagtgg cgtatattga
    15061 tggcggtatt ctgcaggaaa ttcatattga acgtgaggcg cgacgcggaa tagtaggcaa
    15121 tatctacaag ggtcgtgtaa gtcgtgtact tccgggtatg caggcggctt ttgtagatat
    15181 tgggctggat aaagccgcgt ttcttcatgc atccgacatc atgccgcata ccgaatgtgt
    15241 ggcgggtgaa gaacaaaagc aattcacggt gcgcgacatc tcggaactgg ttcgtcaggg
    15301 gcaagatctg atggtgcagg tggtgaaaga tccgcttggc actaaaggtg cgcgcctgac
    15361 caccgatatc acgctgcctt ctcgctatct ggtatttatg ccaggggctt ctcacgttgg
    15421 ggtttcccaa cgaattgaaa gcgaatcaga acgtgaacgt ctgaaaaaag tggtcgcaga
    15481 gtattgcgac gagcagggcg ggtttatcat ccgcaccgca gcggaagggg ttggcgaggc
    15541 tgaactggcc tccgatgccg cttatctgaa acgcgtctgg accaaagtta tggagcgtaa
    15601 aaagcgcccg cagacccgtt atcagctgta cggcgaactg gcgctggcgc agcgtgttct
    15661 gcgtgatttt gccgacgccg aactggaccg cattcgcgtt gactcacgcc tgacttacga
    15721 agcattgctg gagtttacct cggagtacat tcccgagatg accagcaagc tggagcatta
    15781 caccggacgc cagccgattt tcgatctctt tgatgtcgaa aacgaaatcc agcgagcgtt
    15841 ggaacgcaaa gtagaactga aatccggtgg ttatctgatt atcgaccaga ccgaagcgat
    15901 gaccaccgtg gacatcaata ccggagcgtt tgtcggtcat cgcaatctgg acgacactat
    15961 tttcaatacc aatattgaag cgacgcaggc tatcgctcgc cagttacggt tgcgtaatct
    16021 gggcgggatt atcattattg atttcatcga tatgaataat gaagatcacc gccgccgcgt
    16081 gctgcattcg ctggagcagg cgttgagcaa agaccgggtg aaaaccagcg ttaatggttt
    16141 ttcggcgctg gggctggtgg agatgacgcg taaacgcacc cgcgaaagca ttgagcacgt
    16201 actgtgtaac gaatgcccaa cctgccacgg tcgcggaacg gtgaaaaccg tggaaacggt
    16261 atgctatgaa atcatgcgcg agattgttcg tgtccaccat gcttacgact ccgaccgttt
    16321 cctggtctat gcttctccgg cagtagccga agccttgaaa ggcgaagagt cacactcgct
    16381 ggcggaagtg gaaattttcg ttggcaaaca ggttaaagta caaattgaac cgctctataa
    16441 ccaggagcag tttgacgtcg taatgatgta aacagatgct gggccgccat ccggcaaagg
    16501 gtttttgagt cacattttta gcagacaagg agtgacgggt gaggcgattg ccggggattt
    16561 tactgcttac tggagccgcg ctcgttgtga tcgctgccct gctggttagc ggcctgcgta
    16621 ttgctttacc gcatcttaac gcctggcgtc cggaaatcct caacaaaata gaatccgcga
    16681 ctggcatgcc ggtagaagcc agtcagctct cagccagctg gcagaatttt ggcccgacgc
    16741 ttgaagcaca cgacatccgt gcagaactaa aagatggcgg cgaattttcg gttaaacgcg
    16801 ttactctggc gctggatgtc tggcagagcc tgttacatat gcgctggcag tttcgcgacc
    16861 tcactttctg gcagctgcgc tttcgcacca acactcctat caccagcggt ggtagcgatg
    16921 acagtctgga agccagtcac atcagcgatc tgtttcttcg tcaatttgac catttcgatc
    16981 tccgcgacag tgaagtcagt tttctgacgc catccggtca gcgcgccgag ctggcgatcc
    17041 cacaactcac ctggctgaac gatccacgtc gacaccgtgc ggaaggcctg gtaagcctct
    17101 ccagccttac cggacagcac ggcgtgatgc aggtgcgcat ggatttgcgc gatgatgagg
    17161 ggttgttaag caatggtcgc gtctggctac aggcggatga catcgacctg aagccgtggc
    17221 tcggtaaatg gatgcaggac aatattgctc tggaaacggc acagttctcc cttgaaggct
    17281 ggatgacgat cgacaaaggc gatgtaaccg gcggtgacgt ctggctgaaa cagggcggtg
    17341 ccagctggtt gggcgagaag caaacgcata cgctgtcggt ggataatctg accgcgcata
    17401 ttacgcgtga aaatccgggc tggcagttct ctattcccga tacacggatc acgatggacg
    17461 gcaaaccctg gccgagcgga gcattgacgc tggcctggat accggaacag gacgttggcg
    17521 gcaaagacaa taaacgcagt gacgaactcc ggattcgcgc tagtaatctg gagctggcag
    17581 gcctggaggg catacgcccg ctggccgcga aactttcacc tgcactgggt gatgtttggc
    17641 gctccacaca accgagcggc aagattaaca ctctggcgct ggatatcccg cttcaggcgg
    17701 cagacaagac ccgttttcag gcatcgtgga gcgatctggc ctggaagcaa tggaaattat
    17761 taccgggtgc ggaacacttc tccgggacgc tttccggcag cgttgaaaat ggtttgctta
    17821 ccgcgtcgat gaagcaggca aagatgcctt acgaaacggt attccgtgcg ccactggaaa
    17881 tcgccgacgg ccaggcaact ataagctggc tgaacaatga caaaggtttc cagctggatg
    17941 ggcgtaatat tgacgttaaa gccaaagccg tccatgcgcg cggcggtttt cgttacctgc
    18001 aacctgctaa cgatgaaccc tggctgggta ttctggctgg catcagtacc gatgatggtt
    18061 cacaagcctg gcgctatttc ccggaaaact tgatgggtaa agacctggtt gattatttaa
    18121 gtggtgcgat tcagggcggt gaagcggata acgcgacgct ggtttatggt ggcaatccgc
    18181 aactcttccc ctataaacac aacgaaggtc agtttgaagt gctggtgccg ctgcgcaacg
    18241 cgaagtttgc cttccagccg gactggcctg cattaactaa ccttgatatt gaactggact
    18301 ttattaacga cggtttatgg atgaaaaccg atggcgttaa tctgggcggc gtgcgcgcga
    18361 gtaatctcac cgcagtgatc cctgactact caaaagaaaa actgctgatt gacgctgaca
    18421 ttaaaggtcc gggtaaagcc gttggccctt actttgatga gacaccgctg aaagattctc
    18481 tgggtgcgac cctgcaagaa ctccagctcg acggcgatgt gaatgctcgc ttacatcttg
    18541 atatcccgct gaacggcgaa ctggtaaccg cgaaaggtga agtgacgctg cgtaataaca
    18601 gtctgtttat caaaccactc gacagcatcc tgaaaaattt gagcggtaaa ttcagcttta
    18661 tcaatggcga tctgcaaagt gaaccactga cagcaagctg gtttaatcag ccgttgaacg
    18721 tggatttttc caccaaagaa ggggcaaaag cctaccaggt tgcggtgaat ctcaacggta
    18781 actggcaacc ggcgaaaacc ggcgttctgc ctgaagcggt gaacgaagca ttgagtggca
    18841 gcgtggcgtg ggatggtaaa gtgggcattg atctgcctta tcatgctggc gcgacctata
    18901 acgtagagct gaacggcgat ctgaataatg tgagtagtca cttaccttca ccgttagcca
    18961 aacctgcggg tgaaccacta gcggtaaacg ttaaggttga tggcaatctc aacagctttg
    19021 aattaaccgg acaggctggt gcggataacc atttcaatag ccgctggttg cttggtcaaa
    19081 agctgacgct cgatcgtgct atttgggcgg cagacagtaa aacgctcccg ccgttgccgg
    19141 aacaaagtgg cgttgaactt aatatgccgc cgatgaatgg tgccgagtgg ctggccctgt
    19201 tccagaaagg cgctgcggag agtgtcggtg gtgcagcgag tttcccacaa cacataacgt
    19261 tacgtacgcc tatgttgtca ctgggaaatc agcaatggaa taacctgagt attgtttcgc
    19321 aaccgacggc aaatggcacc ctggttgagg cgcaagggcg tgaaatcaac gccacgctag
    19381 cgatgcgtaa taacgcgccg tggctggcga atatcaaata tctttattac aacccgagcg
    19441 tggcgaaaac tcgtggtgat tcaacgccgt catcaccttt cccgacaacg gagcgcatta
    19501 acttccgtgg ctggccggac gcacaaatac gatgcacaga gtgctggttc tgggggcaaa
    19561 aattcggtcg cattgacagt gatatcacca tttctggcga tacgttaacg ctgaccaatg
    19621 gactgattga tactggtttc tcgcggctta ctgccgatgg tgaatgggtt aataatccgg
    19681 ggaatgaacg tacctcgctg aaaggaaaac tgcgtgggca gaaaattgat gccgccgcag
    19741 aattttttgg tgtcacgacg cccatacgcc agtcgtcatt taatgtggat tacgatttac
    19801 actggcgcaa agcaccctgg cagccagatg aagcgacgtt gaatggcatc attcatactc
    19861 aactgggtaa aggcgaaatt accgaaatca ataccggaca tgccgggcaa ttgctgcgct
    19921 tattgagcgt agatgccctg atgcgtaagc tgcgttttga tttcagagac acttttggcg
    19981 aagggttcta ttttgactcc attcgcagca ccgcgtggat taaagacggc gttatgcaca
    20041 ccgacgacac gctggtggat ggcctggagg cggatatcgc catgaaaggg tcggtaaatc
    20101 tggtacgccg cgacctgaat atggaagcgg ttgtcgcacc agagatttct gcgacggtgg
    20161 gcgtggctgc ggcttttgcg gttaacccca ttgttggcgc ggcagtgttt gccgccagta
    20221 aagtgctggg gccgctgtgg agcaaagtct ccattttgcg ctatcacatt tcgggtccgc
    20281 tggacgatcc gcaaatcaac gaagtgttgc gccaaccgcg taaagaaaaa gcgcaatgat
    20341 ttgacgaggg cgcgtaattg ccccaatctc atatgataat cgttgccaaa ggccaacgag
    20401 ccggaacata actgtaggcc ggatagggcg ttcacgccgc atccggcagc cgtaaaaaat
    20461 cctctactgc agtaactaac gagtagcaaa aacgatgagt ctgaacctgg taagtgaaca
    20521 attgctagcg gcgaacggcc tgaaacatca ggacttgttc gcgatcctcg gtcaactggc
    20581 cgaacgtcgc cttgattatg gcgatctcta ttttcagtcg agctatcacg aatcctgggt
    20641 tttagaagac cgcattatta aagatggttc ttacaacatc gatcagggcg ttggcgtgcg
    20701 tgcaatcagc ggtgaaaaaa ccggatttgc ttacgctgac caaatcagcc tgctggcgct
    20761 ggaacagagt gcgcaagcgg cgcgcaccat cgtccgtgat agtggtgatg gcaaagtaca
    20821 gacgctgggc gcggtagagc atagcccgtt gtatacctcg gtagatccgc tgcaaagcat
    20881 gagccgtgaa gagaagctgg atatcctgcg tcgcgtcgat aaggttgctc gcgaagcgga
    20941 caagcgcgta caggaagtga ctgccagcct cagtggtgtc tatgaattaa ttctggttgc
    21001 ggccaccgac ggcacgctag cggcggatgt ccgtccgctg gtgcgtcttt ccgtgagcgt
    21061 tctggtcgaa gaagatggca aacgcgaacg cggtgccagt ggcggcggcg gtcgttttgg
    21121 ttatgagttc ttccttgccg atcttgacgg cgaagtccgt gcggatgcat gggcaaaaga
    21181 agcagtgcgt atggcgctgg tcaatctttc tgccgttgct gcaccagcgg gcaccatgcc
    21241 ggtagtactt ggcgcaggtt ggccgggcgt gctgttgcat gaagcggtag gtcacggtct
    21301 ggaaggcgac ttcaaccgcc gtggcacttc agtatttagt ggacaggtcg gggagctggt
    21361 ggcttcagaa ctgtgtaccg tggttgatga cggcacgatg gtcgatcgcc gtggttcggt
    21421 ggcgattgat gatgaaggta cgccaggcca gtacaacgtg ctgattgaga acggcattct
    21481 gaaaggctac atgcaggata aactcaacgc gcgtttgatg gggatgacgc cgactggcaa
    21541 cggtcgccgt gaatcctacg cccatctgcc catgccgcgt atgaccaaca cctatatgct
    21601 gccgggtaaa tcgaccccgc aagaaattat tgaatccgtt gagtacggta tctatgcacc
    21661 gaactttggt ggcggtcagg tggatatcac ctccggcaaa ttcgttttct ccacttcaga
    21721 agcatatctg attgaaaacg gtaaagtaac gaagccggtg aaaggcgcaa cgttgattgg
    21781 ttccggtatc gaaaccatgc agcagatttc gatggttggc aacgacctga aactggataa
    21841 cggcgtgggt gtctgcggta aagaagggca aagtttgccg gttggcgtgg gccagccaac
    21901 gttgaaagtc gataacctga ctgttggcgg tactgcgtaa taatttcatt attttcagaa
    21961 ggataattaa tctttctacg tgcacgaacg gtcccctcgc tcctctgggg ttagggtgag
    22021 gggaacccgt tggcacaggt ttgtagaacg taacagtaca atatgaatta cttctctttc
    22081 ccgcgcccgt gcatctcctg aaacaattta ccgacctcaa caaaataatc cgtcagcgag
    22141 ttgatcacga cctgtacctt cagcggcagc ttatcttttt cggtatataa cgcataaacc
    22201 gggcgtggat ctgactgata acgcggcagc aggatctcca gctccccacg attgatctcg
    22261 ttgatcaccc acatcagcgg cacgtaggcg atcccggcac ccgccgttag ccagcgcacc
    22321 agcgtcatcg gatcattagt cacaaatctc ccttgcggga tcaggcgagt cgagatccct
    22381 tccggtgcaa tcagttcaaa ttcattgtcg ggccgcacgc tgtattcaag ccatgaatgg
    22441 ctacttaaat cggcgggttt ttccggtatg ccgtattgtg tgagatagct tttcgcggcg
    22501 cacaccacca ttggcatcgc gccaagacgg cgggaaaaca ggctggaatc ctgcaacgcg
    22561 ccgacgcgga tcaccacatc cagaccgtcg gcaatcaggt cgggggctgg aattccggta
    22621 accagattgg cgctcaaacc tgggtattct ttcagcattt tggctgtcag cccggcgaga
    22681 acattttgtg ccatagttga agaacagcca atgcgtagcg tcccgatggg ggtgttattg
    22741 aaggcataca gttgctcatg aacatcctgc acttcatgaa gcatacgacg gcagccctgg
    22801 tagtaaattc taccggcttc ggtcaggcca atgctgcgtg tgctacggtt taacagcttt
    22861 acctgcaact catcttccag ttttgacacc gtctgactga tggacgaaac gctcatctgt
    22921 agctgtctgg cggcggcggt aaaagagcca aattcaacta ctttggcaaa caccgacatg
    22981 cgttttagtc gttccattat tcactctgac ttaaaagtga tttagatcac ataatataga
    23041 taacagcata acagttacgc taatatatta aatatcaatc tacagcaatg ttgctctcgc
    23101 ccggctttcc atgcccttct ctgcggcgac agatgctgaa aataataacg cctgctctct
    23161 ctatacaacc aaggtcaaca tgagtctgtt tcccgttatc gtggtgtttg ggctgtcctt
    23221 cccaccgata ttttttgaat tgcttttatc actggcgatt ttctggctgg tgcgccgggt
    23281 acttgtgcca acaggtatct acgactttgt ctggcatccg gcgttgttca acaccgcgct
    23341 ctattgctgc ttgttttatt tgatatcgcg actgttcgtt tgaggttgaa gtgaaaacac
    23401 taataagaaa attctcccgt acggccatca cggtcgtatt agtcattctg gccttcatcg
    23461 caatttttaa tgcctgggtc tattacaccg aatccccctg gacgcgtgac gcgcgcttta
    23521 gcgctgacgt cgttgcgatc gcgccggacg tttctggact cattacccag gtgaatgttc
    23581 atgataacca gctggtgaaa aaaggacaga tactgttcac catcgaccag ccgcgctatc
    23641 aaaaggcgct tgaggaagcg caagccgatg ttgcttatta tcaggtactg gcacaggaga
    23701 aacgccagga ggccggacgt cgtaaccgtc tcggtgtgca ggcgatgtct cgcgaagaga
    23761 tcgaccaggc taacaacgta ctacaaacgg ttctgcatca gttagcgaaa gcgcaggcga
    23821 cccgcgatct ggcaaaactg gatcttgaac gcacggtgat ccgagcgcca gcagatggct
    23881 gggtgaccaa cctcaacgtc tataccggtg agtttattac tcgaggatca acggcggttg
    23941 cgctggtgaa acagaactcc ttctatgtac tggcctatat ggaagaaact aagctggaag
    24001 gggtgcgtcc ggggtatcgt gcagagatca cgccgcttgg cagtaacaaa gtgctgaaag
    24061 ggactgttga tagtgttgcc gctggggtca ccaacgccag cagcacgcgt gacgacaaag
    24121 ggatggcgac catcgactct gaccttgaat gggtgcgttt ggcgcaacgt gttccggttc
    24181 gtattcgtct cgacaaccag caagagaaca tctggcctgc gggcaccact gctacagtgg
    24241 tggtcactgg caaacaagat cgcgacgaaa gccaggattc gttcttccgt aaaatggccc
    24301 atcgcctgcg tgagtttggt taatcacgat gggtattttc tccattgcta accaacatat
    24361 tcgctttgcg gtaaaactgg cgaccgccat tgtactggcg ctgtttgttg gctttcactt
    24421 ccagctggaa acgccacgct gggcggtact gacagcggcg attgttgccg ccggtccggc
    24481 ctttgctgcg ggaggtgaac cgtattctgg cgctattcgc tatcgtggct ttttgcgcat
    24541 catcggcaca tttattggct gtattgccgg actggtgatc atcattgcga tgatccgcgc
    24601 accattattg atgattctgg tgtgctgtat ctgggccggt ttttgtacct ggatatcctc
    24661 gctggtacga atagaaaact cgtacgcgtg ggggctggcc ggttataccg cgctgatcat
    24721 tgtgatcacc attcagccgg aaccattgct tacgccgcag tttgccgtcg aacgttgtag
    24781 cgagatcgtt atcggtattg tgtgtgcgat tatggcggat ttgctctttt ctccgcgatc
    24841 gatcaaacaa gaagtagatc gagagctgga aagtttgctg gtcgcgcaat atcaattaat
    24901 gcaactctgt atcaagcatg gcgatggtga agttgtcgat aaagcctggg gcgacctggt
    24961 gcgacgcacc acggcactac aaggcatgcg cagcaacctg aatatggaat cttcccgctg
    25021 ggcgcgggcc aatcgacgtt taaaagcgat caatacgcta tcgctgacgc tgattaccca
    25081 atcctgcgaa acttatctta ttcagaatac gcgcccggaa ttgatcactg atactttccg
    25141 cgaatttttt gacacgccgg tagaaaccgc gcaggacgtc cacaagcagc tcaaacgcct
    25201 gcggagagtt atcgcctgga ctggggaacg ggaaacgcct gtcaccattt atagctgggt
    25261 cgcggcggca acgcgttatc agcttctcaa gcgcggcgtt atcagtaaca caaaaatcaa
    25321 cgccaccgaa gaagagatcc tgcaaggcga accggaagta aaagtagagt cagccgaacg
    25381 tcatcatgca atggttaact tctggcgaac cacactttcc tgcattctgg gcacgctttt
    25441 ctggctgtgg acgggctgga cttccggcag tggtgcaatg gtgatgattg cggtcgtgac
    25501 gtcactggca atgcgtttgc cgaatccacg catggtggcg atcgacttta tctatgggac
    25561 gctggccgcg ctgccgttag ggctgctcta ctttttggtg attatcccta atacccaaca
    25621 gagcatgttg ctgctgtgta ttagcctggc agtgctggga ttcttcctcg gtatagaagt
    25681 acagaaacgg cgactgggct cgatgggggc actggccagc accataaata ttatcgtgct
    25741 ggataacccg atgactttcc atttcagtca gtttctcgac agcgcattag gacaaatcgt
    25801 cggctgtgtg ctcgcgttca ccgttatttt gctggtgcgg gataaatcgc gcgacaggac
    25861 tggacgtgta ctgcttaatc agtttgtttc tgccgctgtt tccgcgatga ctaccaatgt
    25921 ggcacgtcgt aaagagaacc acctcccggc actttatcag cagctgtttt tgctgatgaa
    25981 taagttccca ggggatttgc cgaaatttcg cctggcgctg acgatgatta tcgcgcacca
    26041 gcgcctgcgt gatgcgccga tcccggttaa cgaggattta tcggcgtttc accgacaaat
    26101 gcgccgcaca gcagaccatg tgatatctgc ccgtagcgat gataaacgtc gtcggtactt
    26161 tggccagttg ctggaagaac tggaaatcta ccaggaaaag ctacgcatct ggcaagcgcc
    26221 accgcaggtg acggaaccgg ttcatcggct ggcggggatg ctccataagt atcaacatgc
    26281 gttgaccgat agttaagtca aaaccgacgc caaaagcgtc ggttttttca tggctatact
    26341 tagcgatgca cggcagaact cgccgcgaaa cgtgacggtg gcaacagatg aatatttatg
    26401 cctttgattt tgatgagatt gagagtcagg aggattttta tcgtgacttt agccaaacct
    26461 ttggtctggc taaagataag gtacgcgatc tcgactcact atgggatgtg ttaatgaacg
    26521 atgtcctgcc gctaccactt gagattgaat ttgttcatct gggagagaaa acgcgtcgcc
    26581 gttttggcgc gttaatattg ctgtttgatg aggcagagga agagctggaa gggcatttgc
    26641 gttttaatgt tcgtcattag cgcaaaaaaa agcccacgaa ccgggggcaa tatcgtcgga
    26701 caaaacgatg agggtttatt tgtacagttc agccgtagcg tgccaggtgt caccgctacg
    26761 agcttcagta atctggtagg ccgttgcgcc tttctcttcc gcttttttgt tcagcatttc
    26821 acgtatatcc attggcgaag acgccacacc acttacggat acggtcccga ttgcttcacg
    26881 attttgtgct tgtgcagcat caatggagtc ggcagcgaat gcaccgaaag agagaacaga
    26941 aagtacgctt aatgcagcta cagtggtttt gattttcatg atttttacct cgacataatc
    27001 ttttagctgg gcctttgttt cgtgacccat atcacaaaat caagt
//
LOCUS       DAEBXN010000054        25702 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.054, whole genome shotgun sequence.
ACCESSION   DAEBXN010000054 DAEBXN010000000
VERSION     DAEBXN010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25702
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.054"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            1..1331
                     /gene="yjeM"
                     /locus_tag="KJL99_002301"
                     /pseudo
     CDS             1..1331
                     /gene="yjeM"
                     /locus_tag="KJL99_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(1482..4793)
                     /gene="mscM"
                     /locus_tag="KJL99_002302"
     CDS             complement(1482..4793)
                     /gene="mscM"
                     /locus_tag="KJL99_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HBD4437011.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNIRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMELVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNRQLEDALKEVNEATHRYLFWASDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     EQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(4815..5783)
                     /gene="psd"
                     /locus_tag="KJL99_002303"
     CDS             complement(4815..5783)
                     /gene="psd"
                     /locus_tag="KJL99_002303"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HBD4437012.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(5880..6932)
                     /gene="rsgA"
                     /locus_tag="KJL99_002304"
     CDS             complement(5880..6932)
                     /gene="rsgA"
                     /locus_tag="KJL99_002304"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HBD4437013.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            7027..7572
                     /gene="orn"
                     /locus_tag="KJL99_002305"
     CDS             7027..7572
                     /gene="orn"
                     /locus_tag="KJL99_002305"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HBD4437014.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            7783..7858
                     /locus_tag="KJL99_002306"
     tRNA            7783..7858
                     /locus_tag="KJL99_002306"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7816..7818,aa:Gly,seq:gcc)
     gene            8016..8091
                     /locus_tag="KJL99_002307"
     tRNA            8016..8091
                     /locus_tag="KJL99_002307"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8049..8051,aa:Gly,seq:gcc)
     gene            8127..8202
                     /locus_tag="KJL99_002308"
     tRNA            8127..8202
                     /locus_tag="KJL99_002308"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8160..8162,aa:Gly,seq:gcc)
     gene            8238..8313
                     /locus_tag="KJL99_002309"
     tRNA            8238..8313
                     /locus_tag="KJL99_002309"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8271..8273,aa:Gly,seq:gcc)
     gene            8547..8600
                     /locus_tag="KJL99_002310"
     CDS             8547..8600
                     /locus_tag="KJL99_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4437015.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(8583..9722)
                     /gene="queG"
                     /locus_tag="KJL99_002311"
     CDS             complement(8583..9722)
                     /gene="queG"
                     /locus_tag="KJL99_002311"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HBD4437016.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGALPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            9721..11268
                     /gene="nnr"
                     /locus_tag="KJL99_002312"
     CDS             9721..11268
                     /gene="nnr"
                     /locus_tag="KJL99_002312"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HBD4437017.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKLGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPSEAARLLGCSVAEIES
                     DRLHCAKPLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            11240..11701
                     /gene="tsaE"
                     /locus_tag="KJL99_002313"
     CDS             11240..11701
                     /gene="tsaE"
                     /locus_tag="KJL99_002313"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HBD4437018.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            11720..13057
                     /gene="amiB"
                     /locus_tag="KJL99_002314"
     CDS             11720..13057
                     /gene="amiB"
                     /locus_tag="KJL99_002314"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HBD4437019.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGMIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVAHKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKLSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            13067..14914
                     /gene="mutL"
                     /locus_tag="KJL99_002315"
     CDS             13067..14914
                     /gene="mutL"
                     /locus_tag="KJL99_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HBD4437020.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            14907..15857
                     /gene="miaA"
                     /locus_tag="KJL99_002316"
     CDS             14907..15857
                     /gene="miaA"
                     /locus_tag="KJL99_002316"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HBD4437021.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSV
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            15943..16251
                     /gene="hfq"
                     /locus_tag="KJL99_002317"
     CDS             15943..16251
                     /gene="hfq"
                     /locus_tag="KJL99_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HBD4437022.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            16327..17607
                     /gene="hflX"
                     /locus_tag="KJL99_002318"
     CDS             16327..17607
                     /gene="hflX"
                     /locus_tag="KJL99_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBD4437023.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKAMGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            17693..18952
                     /gene="hflK"
                     /locus_tag="KJL99_002319"
     CDS             17693..18952
                     /gene="hflK"
                     /locus_tag="KJL99_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HBD4437024.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYSNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            18955..19959
                     /gene="hflC"
                     /locus_tag="KJL99_002320"
     CDS             18955..19959
                     /gene="hflC"
                     /locus_tag="KJL99_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HBD4437025.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            20041..20238
                     /locus_tag="KJL99_002321"
     CDS             20041..20238
                     /locus_tag="KJL99_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HBD4437026.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            20342..21640
                     /gene="purA"
                     /locus_tag="KJL99_002322"
     CDS             20342..21640
                     /gene="purA"
                     /locus_tag="KJL99_002322"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HBD4437027.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPAELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            21845..22270
                     /gene="nsrR"
                     /locus_tag="KJL99_002323"
     CDS             21845..22270
                     /gene="nsrR"
                     /locus_tag="KJL99_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HBD4437028.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            22309..24750
                     /gene="rnr"
                     /locus_tag="KJL99_002324"
     CDS             22309..24750
                     /gene="rnr"
                     /locus_tag="KJL99_002324"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HBD4437029.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRCQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            24930..25661
                     /gene="rlmB"
                     /locus_tag="KJL99_002325"
     CDS             24930..25661
                     /gene="rlmB"
                     /locus_tag="KJL99_002325"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HBD4437030.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
BASE COUNT         6019 a         6515 c         7215 g         5953 t
ORIGIN      
        1 ttaatgatgg ctgaaatggg agctgcttat cgcaaagaag aaggtggtat ctattcctgg
       61 atgaataata gtgtcggacc acgttttgcc ttcattggta cgtttatgtg gttttcctct
      121 tatatcatct ggatggtgag tacctccgcg aaagtttggg taccgttctc aacattcctc
      181 tatggtagtg acatgaccca gcactggcgt attgccggac tggagcctac gcaggtggtt
      241 ggtctgctgg cagtagcatg gatgattctg gtcacagtcg ttgcttcaaa ggggattaat
      301 aaaattgccc gcattactgc ggtgggcggt attgcagtaa tgtgtctgaa tttagtattg
      361 ctgttagtaa gcattactat tttgttatta aatggtgggc atttcgcgca ggatattaat
      421 ttccttgcat caccgaaccc gggttatcag tccggtctgg caatgctatc gtttgtggta
      481 tttgctattt ttgcctatgg cggaattgaa gcggttggtg gtctggtcga taaaacggaa
      541 aatccagaaa agaactttgc caaaggtatt gtttttgccg ctattgttat ttcaatcggt
      601 tattcgctgg caatattttt atggggcgtc agcacaaact ggcagcaggt attaagtaat
      661 ggttccgtta accttggcaa tattacctat gtgctgatga agagcctcgg ggtgatgctg
      721 ggtaacgcac tgcatttgtc acctgaagcg tcattgtcgc tgggcgtatg gtttgcgcgt
      781 attaccggac tttcgatgtt cctcgcttat accggtgcgt ttgctattca ccgctgaaag
      841 ccatcatcca ggggacgccg aaagcgttgt ggccggaacc gatgacgcgc ctgaatgcga
      901 tggggatgcc gtctatcgcc atgtggatgc agtgcgggtt ggttactgtc ttcattctgc
      961 tggtttcgtt tggtggcggt accgcatcgg cgttctttaa caagctgacg ctgatggcga
     1021 acgtgtctat gacgcttcct tacctgttcc tcgcgctggc tttcccgttt tttaaagctc
     1081 gtcaggatct cgacagacca tttgtgattt tcaaaacgcg tatgtcggca atgattgcga
     1141 cggtggttgt cgttctggtg gtgacatttg cgaacgtctt caccattatt cagcctgtgg
     1201 ttgaagccgg agactgggac agcacattgt ggatgattgg cggccctgtc ttcttctcgc
     1261 tgttagcgat ggcgatttac cagaactatt gcagtcgcat ggcgaataaa cctgagttag
     1321 ctctcgactg atcatatgaa atcctgcaac tcatcgtctc tccggcgatg agttgtcagg
     1381 aagtaatgcg tatggatgat actctccagg gccggtgata actgaagatg atgttaagct
     1441 gctaaatgag ccacgttgac atcatttggc agcttcatgc attacaaact tcccgcctga
     1501 cgacctttgc ccgcagacgt cagcgttctc cccgtttgtt taccgttgag gctttccaga
     1561 cgcatctgga agggcgggaa tggcatatcg ataccgtggg cgtggaagcc tgccagaatc
     1621 agctggtgga tctcatggcg tagcggcata cggtgaccca tctcagcggc gtaaatacgc
     1681 agctcgaaaa tctgaatccc ctgttgcaga tccaccagga agacttccgg tgccgggttg
     1741 tcgatcacca gcgagcagcg acgcgctgcg gtgagcagga tttccgtcac ttcttcactg
     1801 ttggcatcgg caggggccgg aatcgtcaac actacgcgcg tgaccgagtc agagagcgac
     1861 cagttgataa actgctcggt aataaacgcc ttgttcggca cgattatctc tttacggtcc
     1921 cagtcgctga tggtggtggc gcgggtgtta atttttgtca cgctaccagt aagatcgcgg
     1981 atcgttaccg tatcgccaat gcgaatcggt ttttcgaaca ggatgatcag gccagagata
     2041 aagttggcga aaatttcctg caaaccaaaa ccgagaccaa caccgagcgc ggcaaccagc
     2101 cactgcaatt tcgaccactc aataccaatc attgagaagc cgaccagccc gccaatcagc
     2161 atcagcagat attttgtgat ggtggtgatg gcgtaacccg tacccggcgt taaatccagg
     2221 tgctgcaaaa tcgccagttc cagcagcgcg ggcaggttgc gcaccagctg cgtggtgatg
     2281 ataaatacca gaatggcaat cagcaccgca ccgagggtaa ttggctccag actttctacg
     2341 ccctgtaccg tggaggtgac atcccacagc gaaatatttt cgaggaagcc gaaagcggaa
     2401 tggatttctg accacagcac aatgaccgaa agcagggcga tcaacatcaa aattgagcgc
     2461 accagccgca aggattgcgc actgatggca tcgagatcga cttcgctttc atcgacttca
     2521 attgctcctt ccgggctact gtgatgatgc gcttcctctt cgccacgcgc acgttgcgct
     2581 aacatctctg cccggcgatg cttcgcccgg tcaaacgcca gcctgcgacg ctggatcagc
     2641 atccagcggc ggataacgtg ataaaccacc agtagcagga accagatggc aaccgaggtt
     2701 tcaagcctcg ctaacagtgc ctgcgccgtt gccagataac ccaccgccga cgccagaatg
     2761 gcaaccagtg gtgcgccaat catcatgttc cacagcatat ggttggtaat gttgtcgccg
     2821 ctgccttctt tgttgagata caacggaata ccggcttttt tcaggcttag ggtgaccacc
     2881 gccagcgcac cgcaaatgag gataaagcaa agccgtccca gcgaaccgga aaattcgcgg
     2941 tcgtcgaggt tatcgaacat catcagcgcc ataatcagcc cgatgctcat caggtagtag
     3001 cgcatcccac gggaaacacg ttcgcgcggc cagccaaaat gagcgataaa caagccgttc
     3061 gggcgggcaa aggtggcgca aatcatcact acccacagca gcggcacggt ggccgttaca
     3121 ccatcgccaa tcgcgaccgc cagcggataa ggccacgcct cgcgcaagcc gtaacccagc
     3181 gtcatccaca gcaccggtaa cggtgacgcg acgagaatcg accagaaaag agtgcgcaac
     3241 gtcagccaga agtgatcctg agtcactttg ccgactttcg ccgccgaacg ttcaagaaaa
     3301 cgggtgaaat agcggcgcga gtaaatactg caaccgacca gaatcaacgc gccaaacagc
     3361 ggcaaaattg tctctttgct ggtcagcatc atcacactgg ctttgcccaa ctgactgaag
     3421 gtgtccagcg aaatgagacg acgcagatcc tgggcgattt ccagcggcca ggcgatggtc
     3481 atcgggcgca cgtcagaggc ccagaacaga tagcggtgcg ttgcttcgtt cacctctttc
     3541 agcgcatcct ccagttgcct gttggagact ttcagcttgg tcagttccaa tagtagcgtg
     3601 tcgccaccct gcaacaatga gttcagcaac tcacgctgag tgcgcagttg tgcttccaga
     3661 atacggtttt gctcggcggt cagcggctga ccgtcggcct gatggatttg ccgtagcagc
     3721 ggctgtttat taagtaaatc ctcataacgt aaccgttgca cacgcaactg cgccatttcg
     3781 gtatcaagct gttgtggttt tggcatttct ggtagccgtg ccacctgcgc ccgcagcgct
     3841 tcgccgagca ggttggacga tcccagccat tgcgactgtt cacgcagcgt attcaacgcc
     3901 tgccggacct gtaacgtctg gctggcagcc tgacgctgtt gcgaggcaac gagctccatc
     3961 cgctgcgcct gttgattcag cgctgccgat agttcgcggt taattttgaa ttgcgcgacg
     4021 atatctttcg gcaaatcggc actgttttct gccagtaatt cggtactttc cagcgcccgc
     4081 tccgcctcaa gctgacgttg gctattcaac tgattacgta aggcctgcaa atacgcatcc
     4141 agttgctggc tctctttttc cgccagctct gagcgtaagc gcgctaattc ctggcggtta
     4201 ttggcagaca gctgcgccag ctccagttca tcaacgagcg ccttaagacg tgcagagtca
     4261 gactgcaacg cgaaattttg tgcctgattg agcggagtat tgccggtaag cgttcccagg
     4321 cggcgctcga tctcatttaa ctgacggcgg gcgtcggttt gctgttgcgg cagttgattc
     4381 agcgaatcgg caatctcgcg ggcgcgctcc tgctcttgct gggcctgacg gcttttatcc
     4441 agcaactggc tgctgacctg gagaatttcc tgattcagcg cgtcggtaga cattcccggc
     4501 gacacgctgc gcggctcgtc acgaatgttg tttaattgtg cgcgcagagt agcggagagt
     4561 ttcggataat tatcgataac ttgctgatat tgtttgatgc gctcaaggga accttttcgt
     4621 tcctcaagcg catttaaggc agactggagc gcctctacga cttccggctg tgcgggtttc
     4681 gccgcttttg cctgctccag ttcctgagtg atttgtttgc tatcgggggc cgtcgcggcg
     4741 tacgcccccc aactgaggca ccaggccatc agaaaagtga taatcaggcg cacgtcagcg
     4801 tttcctttga tggattagac ctggtctttt ttatcgtcaa ccaatgggct ggcgtcgtgt
     4861 tctgcttcga tctcttcagc aggaagtggg gcgggttcag cgtctggcgt aacaaaggtt
     4921 tcggtagata ctgccagcgg ctggccaatt ttcgtgacag acaggctttc cagttgctca
     4981 accagattca ctttacccgg tgcaaacagg ttgataacgg tggaaccgag tttaaagcga
     5041 cccatttcct ggcctttcag cagtgccaca gaaccgtcgt tttccccggc aggccaggtc
     5101 cagcgcttga tgataccttc gcgcggcggc gtaatggtgc ccgcccagac cgtctcaatg
     5161 ctgccaacaa tcgtcgctcc gaccagaatc tgcgccattg ggccaaattc ggtatcgaaa
     5221 aggcaaatca cgcgttcgtt acgggcaaac agatttggca cgttctgagc ggtgagatgg
     5281 ttaacggaaa agagatcgcc cggcacgtag atcatctcac gcagaatacc gttgcacggc
     5341 atgtgtacgc gatggtagtc acgcggggag aggtaagtgg tcacaaacgt accgttgcgg
     5401 aacaggtccg ccatcagata gttgcctgcc agcagggctt cgaggctgta gttgtggcct
     5461 ttggcttgca ggattttatc ttcttcgatt ttacccaact ggctgataac gccatcggca
     5521 ggcatgacca gtacattcgg atcggtatcg attgggcgta cttcgtcacg cagcggacgg
     5581 acaaagaatt cgttaaaggt gcggtagctg gcggtgtccg gcttttgcgc ctctttcatg
     5641 tcgaccttgt agtatttaac gaacagatcg ataaccagtt ttgtcagcca tcctgcccgc
     5701 ttgcttgcgc cccaacccgc caggcgagta agccatagtt tcggcagaat gtactgtagc
     5761 gaaagtttaa atgaatttaa caaggtagcc tccaggccat tgttttgtcg ttcctgatcc
     5821 ggccaacatg ccggatcctg aaaaaaaggg gacgattcta acgacggtta gcttaattgt
     5881 cagtcatccg tatcagaaaa gtttttacgc gtttttacct gcgccatgct ttccagaata
     5941 cggtgatagt tttcgaaacg ggtttccgcg attttccctt cctcaaccgc ttcccggata
     6001 gcgcagcccg gatcggtatc gtgtttgcaa tcgcgatatt tacacagacc taaatagtca
     6061 tggaattcga caaagccctg agtgatttgt tccggctcca ggtgccagag gccgaactca
     6121 cgcacaccag gggaatcaat cacatcaccg ccgtgcggga agtgatacag ccgagcggcg
     6181 gtcgtggtgt gctgaccgag ccccgagttg tcagagacat cgttggtcag gatctctttt
     6241 tgcagcccca gcaacgcgtt cagcaggctg gatttaccga cgccagactg cccggcaaaa
     6301 atgctgatgc gcccggtcaa cgcctcttcc agcggtttca gcccatcctg agtatggctg
     6361 gaaaccatca atacgcgata accgatgttg cggtagatat ccatctgctc gttgacgaac
     6421 gccatgcctt cgtcgtccag caggtctatc ttgttgagca cgataatcgg ctcaatctgc
     6481 aaggtttcgc aggccaccag gtaacggtcg ataatattga gcgacagctc cggcaaaatg
     6541 gcggagacaa tgacaatctg gtcgatgttg gcggctatgg gtttcacgcc gtcgtagaaa
     6601 tccggacgcg ttaacaccga ggtgcgctca tgcaccgctt ccacgatccc tttgacattt
     6661 acgccttccg ccgccggttt acccggacgc cagactacgc ggtcgccggt taccagcgaa
     6721 cggatggtac ggcgaatatt gcagcggtga acgtcgccat cggcggattc cacatcagcg
     6781 tgcataccaa agcggctgat gacgatacct tcatcaggct cgccaaacag attgtcgtcg
     6841 tagtcgggct tctccttaga cgttttaaga cgacgctggt gattggcgtt cacgcggcgc
     6901 tgctggcctt tggagagttt atttttactc aatcgtactg actcctggtc gcccctgatg
     6961 ggcaaaacat ctatgataca cgcaattgtg gatcaatata gtcactgtga atgggtggaa
     7021 aatagcatga gtgccaatga aaacaacctg atttggatcg atcttgagat gaccggtctg
     7081 gatcccgagc gcgatcgcat tattgagatt gccacgctgg tgaccgatgc caatctgaat
     7141 attctggcag aagggccgac cattgcagta catcagtccg atgaacagct ggcgctgatg
     7201 gatgactgga acgtgcgcac ccataccgcc agcgggctgg tagagcgcgt gaaagcgagc
     7261 acgatgggcg atcgggaagc tgaactggca acgctcgaat tcttaaaaca gtgggtgcct
     7321 gcgggaaaat cgccgatttg cggtaacagc atcggacagg accgtcgttt cctgtttaaa
     7381 tacatgccgg agctggaagt ctacttccac taccgttatc tcgatgtcag caccctgaaa
     7441 gagctggcgc gccgctggaa gccggaaatt ctggatggtt ttaccaagca ggggacgcat
     7501 caggcgatgg atgatatccg tgaatcggtg gcggagctgg cttactaccg cgagcatttt
     7561 atcaagctgt aaaatttttc ctggtcacgt aagcgccgga atccacttgc ggcggcgtga
     7621 ttttgacgct aaatcgaacg tcttgctgat aatttcagca cttgagataa aaacgcaaaa
     7681 aaaacttttt tggggggttg cagagggaaa gatttctcgt ataatgcgcc tcccgtaacg
     7741 acgcagaaat gcgaaaatta cgaaagcaaa attaagtagt acgcgggaat agctcagttg
     7801 gtagagcacg accttgccaa ggtcggggtc gcgagttcga gtctcgtttc ccgctccaaa
     7861 atttgaaaag tgctgcaaag cacagaccac ccaatcgcag tatttcgaat tgcgacaagg
     7921 cggcaactga acgaatcctt gggaacttac aaaagtatgt gactaaggtg agtgaaggca
     7981 gccaaggcag tagcggttcg aaagacgaag attaagcggg aatagctcag ttggtagagc
     8041 acgaccttgc caaggtcggg gtcgcgagtt cgagtctcgt ttcccgctcc aaaatttaaa
     8101 agtgctgtaa ggcatagacc acccaagcgg gaatagctca gttggtagag cacgaccttg
     8161 ccaaggtcgg ggtcgcgagt tcgagtctcg tttcccgctc caaaatttga aagtgctgta
     8221 aggcacagac cacccaagcg ggaatagctc agttggtaga gcacgacctt gccaaggtcg
     8281 gggtcgcgag ttcgagtctc gtttcccgct ccaaattctt ctctcaataa aatatctaca
     8341 gcgacgcgat gcgttattgc tggtttttgt tgtctctgac aaactcttgt aaacagagtt
     8401 atccacagcc tcaggctgta atcttaattt caaagaaact tcgcacggtg aatggtattt
     8461 ttttaaccta ttgataaata agttaaaaat caagattccg ttttgtcgag tcgatcactt
     8521 gacgatttta ttcgtcttga attgcaatgc gtttttattt ttattcacaa gctgtggatg
     8581 aatcaggcgt cacgcggtaa ccctttttca atcacccgaa ccagacgctg ttttttcggc
     8641 aattgcactt cgactatgca cgcatttcgt ctctctattt gctgcgcaat cgcccacgct
     8701 atgtgctcat cgagaagtgg gtgctcacct ttacgacttt ccagcgctgt caaaatcgtt
     8761 tcatcccaag gggcattgcc taatgccacg gcgatattac gcagccagcg caggtgacct
     8821 atacggcgaa tcgccgatcc ttccgtgact tttaaaaact tctcttcgct ccaggcaaat
     8881 aactcaatga gttccggtgc gtgtagcggc ttacgcgggc tgaaatcgtc ttctgtagtg
     8941 agttgcgaat agcgattcca cgggcagata agctggcagt catcgcaacc gtaaatacgg
     9001 tttcccatta acggtcgcaa ctcttccggg agcgcccctt caagttcgat ggtgagataa
     9061 gagatacagc ggcgagcatc gacggtatat ggctcgacga tggcaccggt cgggcaaatc
     9121 gtcatacagg ccacgcattt gccgcatcct tcctcgactg gttgatccac gggcagcgga
     9181 atatcgacca gcaattcgcc taaaaagaag aacgaaccgg cctcgcgatt gaggataagt
     9241 gagtgcttac ctgtccagcc gagcccagct ttttcagcta acgggcgctc gagaataggc
     9301 gcagaatcga caaacggtct aaaattcagc gaaacacaat gttgctgaat catctcgccc
     9361 agctttttga gtcggttgcg cagaagtttg tgatagtcac ggcccagcgc ataacggcta
     9421 acatagccga gtttggggtt tttcagcgtg ctggcaaatg cggcgttagc aggaaggtaa
     9481 ttcatccgca cgctgattac acgcagcgta ccgggtaata actcatgggg gcgagcgcgc
     9541 agcataccgt gacgtgccat ccagtccatt tcgccgtggt attgtttgtc cagccatgct
     9601 tgcagtttgg gctcggactc gctgagatcg gtatcggtaa tacctacctg ctgaaagccc
     9661 agttccagcc cccactgttt aattttttgc gctaactgat tgagatcgag gggctctgac
     9721 atgacggacc atacaatgaa gaaaaacccc gtaagtatac cacacaccgt ctggtacgcc
     9781 gacgatatcc gccgcggaga acgcgaggcg gcagatgtgc tggggctcac actctatgag
     9841 ctgatgcttc gcgctggcga ggccgcattc caggtgtgtc gttcggcgta tcctgacgcc
     9901 cgccactggc tggtgctgtg cggtcatggt aataacggcg gcgatggcta cgtggtcgcg
     9961 cgactggcca aagcggtcgg cattgaggtc acgttgttgg cccaggagag cgacaaaccg
    10021 ttgccggaag aggccgcgct ggcacgcgaa gcatggttaa acgcgggtgg cgagatccat
    10081 gcttcgaata ttgtctggcc cgaatcggta gatctgattg ttgatgcgct gctcggtacc
    10141 ggcttgcagc aagcgccccg cgaatccatt agccagttaa tcgaccacgc taattcccat
    10201 cctgcgccga ttgtggcggt tgatatccct tccggcctgc tggctgaaac cggcgctacg
    10261 ccaggcgcag tgatcaacgc cgatcacacc atcactttta ttgcgctgaa actaggcttg
    10321 ctcactggaa aagcgcggga tgttacagga caactgcatt ttgactcact ggggctggat
    10381 agctggctgg caggtcagga gacgaaaatt cagcggtttt cggcagaaca actttctcac
    10441 tggctgaaac cgcgtcgccc gacttcgcat aaaggcgatc acgggcggct ggtgattatc
    10501 ggtggcgatc acggcacggc gggggctatt cgtatgacgg gggaagcggc gctacgtgct
    10561 ggtgctggtt tagtccgagt actgacccgc agtgagaaca ttgcgccgct gctgactgca
    10621 cgaccagaat tgatggtgca tgaactgact atggactctc ttaccgaaag cctggaatgg
    10681 gccgatgtgg tggtgattgg tcccggtctg ggccagcaag agtgggggaa aaaagccctg
    10741 caaaaagttg agaattttcg caaaccgatg ttgtgggatg ccgatgcatt gaacctgctg
    10801 gcaatcaatc ccgataagcg tcacaatcgc gtgatcacgc cgcatcctag cgaggccgca
    10861 cggttgttag gctgttccgt cgctgaaatt gaaagtgacc gcttacattg cgccaaacct
    10921 ctggtacaac gttatggcgg cgtagcggtg ctgaaaggtg ccggaaccgt ggtcgccgcc
    10981 catcctgacg ctttaggcat tattgatgcc ggaaatgcag gcatggcgag cggcggcatg
    11041 ggcgatgtgc tctctggtat tattggcgca ttgcttgggc aaaaactgtc gccgtatgat
    11101 gcagcctgtg caggctgtgt cgcgcacggt gcggcagctg acgtactggc ggcgcgtttt
    11161 ggaacgcgcg ggatgctggc aaccgatctc ttttccacgc tacagcgtat tgttaacccg
    11221 gaagtgactg ataaaaacca tgatgaatcg agtaattccg ctccctgatg agcaggcaac
    11281 attagacctg ggcgagcggg tagcgaaagc ctgcgatggc gcaaccgtaa tctatctgta
    11341 tggcgattta ggcgcaggta aaaccacctt tagccggggc tttttacagg ctctgggtca
    11401 tcagggtaat gtcaaaagcc ccacttatac gctggtcgaa ccctatacgc tcgacaactt
    11461 aatggtctat cactttgatt tgtaccgcct tgccgatccc gaggagctgg agtttatggg
    11521 gatccgcgat tattttgcca acgatgccat ctgcctggtg gagtggccac aacaaggtac
    11581 aggtgttctt cctgacccgg atgtcgaaat acacattgat tatcaggcac aaggccgtga
    11641 ggcgcgcgtg agtgcggttt cctctgcggg tgaattgttg ctggcgcgtt tagccggtta
    11701 acctttgaaa ggtggcggga tgatgtatcg catcagaaat tggttggtag cgacgctgct
    11761 gctgctgtgc acgccggtgg gtgccgcgac gctctctgat attcaggttt ctaacggtaa
    11821 tcaacaggcg cggataacgt tgagttttat tggcgatcct gattatgcgt ttagccatca
    11881 aagcaaacgc accgtggcgc tcgatatcaa acaaacgggc atgattcagg gactgccgtt
    11941 gttgttcagc ggcaataatc tggtgaaggc gattcgctct ggaacgccta aagatgcaca
    12001 aacgctacgg ctggtggtcg atcttaccga aaacggtaaa accgaagcgg tgaagcggca
    12061 gaatggcagc aattacactg tcgtctttac gattaacgcc gatgtgccgc caccgcctcc
    12121 tccgccgccc gtggttgcga aacgcgttga aacgcctgcg gttgtcgcac cgcgcgtcag
    12181 cgaaccggcg cgcaatccgt ttaaaacgga aagtaaccgc actacgggtg ttatcagcag
    12241 taatacggta acgcgtccgg cagcgcgcgc gacggctaac actggcgata aaattatcat
    12301 cgctattgat gccggacacg gcggtcagga ccctggcgct atcggccccg gtggtacgcg
    12361 ggagaaaaat gtcaccatcg ccatcgcgcg taaattgcgt actttgctca atgacgatcc
    12421 gatgtttaaa ggcgttttaa cccgtgacgg ggattacttt atctcggtga tggggcgcag
    12481 tgatgtggca cataagcaaa acgccaattt cctcgtgtcg attcacgctg atgccgcacc
    12541 gaaccgtagt gcgactggcg cttccgtatg ggtgctctct aaccgtcgcg ccaacagtga
    12601 aatggccagc tggctggagc agcacgagaa actgtcggag ctgctgggtg gggcgggtga
    12661 tgtgctggcg aacagtcagt ctgaccccta tttaagccag gcggtgctgg atttacagtt
    12721 cggtcattcc cagcgggtag ggtatgatgt agcgaccagt atgatcagtc agttgcaacg
    12781 cattggcgaa atacataaac gtcgaccaga acacgccagc cttggcgttc tgcgctcgcc
    12841 ggatatccca tcagtactgg tcgaaaccgg ttttatcagc aacaacagcg aagaacgttt
    12901 gctggcgagc gacgattacc aacaacagct ggcagaagcc atttacaaag gcctgcgcaa
    12961 ttacttcctt gcgcatccga tgcaatctgc gccgcagggt gcaacggcac aaactgccag
    13021 tacggtgacg acgccagatc gcacgctgcc aaactaagga cgattgatgc caattcaggt
    13081 cttaccgcca caactggcga accagattgc cgcaggtgag gtggtcgagc gacctgcgtc
    13141 ggtagtcaaa gaactagtgg aaaacagcct cgatgcaggt gcgacgcgta tcgatattga
    13201 tatcgaacgc ggtggggcga aacttatccg cattcgtgat aacggctgcg gtatcaaaaa
    13261 agatgagctg gcgctggcgc tggctcgtca tgccaccagt aaaatcgcct ctctggacga
    13321 tctcgaagcc attatcagcc tgggctttcg cggtgaggcg ctggcgagta tcagttcggt
    13381 ttcccgcctg acgctcactt cacgcaccgc agaacagcag gaagcctggc aggcctatgc
    13441 cgaaggacgc gacatggacg tgacggttaa accggcagcg catccggtgg gaacgacgct
    13501 ggaggtgctg gatctgttct acaacacccc ggcgcggcgc aaattcctgc gcaccgagaa
    13561 aaccgaattt agccatattg atgaaatcat ccgccgcatt gcgctggcgc gtttcgacgt
    13621 cacgatcaac ctgtcgcata acggtaaaat tgtgcgtcag taccgcgcag tgccggaagg
    13681 cgggcaaaaa gaacggcgct taggcgcgat ttgcggcacc gcttttctcg aacaagcgct
    13741 ggcgattgaa tggcaacacg gcgatctcac cctgcgcggc tgggtggccg atccaaatca
    13801 cactacgccc gcactggcgg aaattcagta ttgctacgtg aatggtcgca tgatgcgcga
    13861 tcgcctgatc aatcatgcga tccgccaggc ctgcgaagac aaactggggg ccgatcagca
    13921 accggcattt gtgttgtatc tggagatcga cccgcatcag gtggacgtca acgtacaccc
    13981 cgccaaacac gaagtgcgtt tccatcagtc gcgtctggtg catgatttta tctatcaggg
    14041 cgtgctgagc gtgctacaac agcaactgga aacgccgcta ccgctggacg atgaaccaca
    14101 acccgcaccg cgtgccatcc cggaaaaccg cgtggcggcg gggcgcaatc actttgcaga
    14161 accggcagct cgtgagccgg tagctccgcg ctactctcct gcgcccgcct caggcagtcg
    14221 tccggctgcc ccctggccga atgcgcagcc aggctaccag aaacagcaag gtgaagtgta
    14281 tcgccagctt ttgcaaacgc ccgcgccgat gcaaaaacca aaagcgccgg aaccgcagga
    14341 acctgcactt gcggcgaaca gtcagagttt tggtcgggta ctgactatcg tccattccga
    14401 ctgtgcgttg ctggagcgcg atggcaacat ttcactttta tccttgccag tggcagaacg
    14461 ttggctgcgt caggcacaat tgacgccggg tgaagcgccc gtttgcgccc agccgctgct
    14521 gattccgttg cggctaaaag tttctggcga agaaaaatcg gcattagaaa aagcgcagtc
    14581 tgccctagcg gaattgggta ttgatttcca gtcagatgca cagcatgtga ccatcagggc
    14641 cgtgccttta cccttacgcc aacaaaattt acaaatcttg attcctgaac tgataggcta
    14701 cctggcgaag cagtccgtat tcgaacctgg caatattgcg cagtggattg cacgaaatct
    14761 gatgagcgaa catgcgcagt ggtcaatggc acaggccata accctgctgg cggacgtgga
    14821 acggttatgt ccgcaacttg tgaaaacgcc gccgggtggt ctgttacaat ctgttgattt
    14881 acatccggcg ataaaagccc tgaaagatga gtgatatcag taaggcgagc ctgcctaagg
    14941 cgattttttt gatggggccg acggcctccg gtaaaacggc gttagccatt gagctgcgta
    15001 aaattttacc agtagagttg ataagcgttg attctgtcct tatttacaaa gggatggata
    15061 tcgggacggc gaagccgaac gctgaagagt tactcgccgc gccgcaccga ttgctggata
    15121 ttcgcgatcc gtcgcaggct tactcggctg ctgattttcg ccgcgatgcg ctggcggaaa
    15181 tggccgatat caccgcggcg gggcggatcc cactgttagt gggcggtacg atgttgtatt
    15241 tcaaggcatt gctggaaggg ttgtcgccgc taccgtcggc agacccggaa gtgcgggcca
    15301 gaattgagca acaggcggca gagcaaggtt gggagtcatt gcatcgtcaa cttcaggagg
    15361 tagatccggt tgcggcagca aggattcatc caaatgatcc acaaaggctt tcccgggcac
    15421 tggaagtttt tttcatttcg ggtaaaactt taacggaact gacgcaaacg tcaggagacg
    15481 ctctaccgta tcaggtgcat cagttcgcca tcgccccggc gagccgtgaa ctgctccatc
    15541 aacgcattga gcagcgtttt catcagatgt tggcttcagg ttttgaagca gaagtccggg
    15601 cgctttttgc ccgaggagat ttgcatacgg acttgccttc cattcgttgc gtgggttatc
    15661 gccagatgtg gtcttacctt gaaggcgaaa tctcatacga tgaaatggtt tatcgaggtg
    15721 tttgcgccac gagacagttg gcgaagcggc agataacctg gctgcgtggt tgggaagggg
    15781 ttcactggct tgacagtgaa aaaccagaac aggcgcgtga cgaagtatta caggttgttg
    15841 gtgctatcgc aggctgaatg tgtacaattg agacgtatcg tgcgcaattt tttcagaatc
    15901 gaaaggttca aagtacaaat aagcatataa ggaaaagaga gaatggctaa ggggcaatct
    15961 ttacaagatc cgttcctgaa cgcactgcgt cgggaacgtg ttccagtttc tatttatttg
    16021 gtgaatggta ttaagctgca agggcaaatc gagtcttttg atcagttcgt gatcctgttg
    16081 aaaaacacgg tcagccagat ggtttacaag cacgcgattt ctactgttgt cccatctcgc
    16141 ccggtttctc atcacagtaa caacgccggt ggcggtacca gcagtaacta ccatcatggt
    16201 agcagcgcgc agaatacttc cgcgcaacag gacagcgaag aaaccgaata aggtttcggg
    16261 ctgttttttt acacggggag ccagcgatcc tgcgttcccc gctgatctat ttagagggtt
    16321 atacgcttgt ttgaccgtta tgatgctggt gagcaggcgg tactggtaca catctatttt
    16381 acgcaagaca aagatatgga agacctccag gagtttgaat ctctggtctc ttccgccggt
    16441 gtcgaagcat tgcaggtgat taccggtagc cgtaaagcgc cgcacccaaa gtattttgta
    16501 ggtgaaggta aagcagttga aattgcggaa gctgtcaaag cgatgggtgc ttcggtcgtt
    16561 ctttttgacc atgccctgag cccggcgcaa gagcgtaacc tggagcgttt gtgcgagtgt
    16621 cgtgttatcg accgcaccgg ccttatttta gatattttcg cccaacgtgc gcgtacccat
    16681 gagggtaagt tgcaggttga gctggcgcag ctgcgccatc tggctacgcg cctggtgcgt
    16741 ggctggaccc accttgaaag acagaaaggc gggataggtt tgcgtggtcc gggtgaaacc
    16801 cagctcgaaa ccgaccgtcg tttgttgcgt aatcgcatcg tgcagataca gtcgcgcctg
    16861 gaaagagttg aaaagcagcg tgagcagggg cggcaatcgc gtatcaaagc cgacgttcct
    16921 actgtttcgc tggtgggata taccaacgcc ggtaaatcta cccttttcaa tcgcatcacc
    16981 gaagcgcggg tctacgcggc agaccagttg tttgccaccc tcgacccgac gttgcggcgt
    17041 attgacgttg cagatgtcgg tgaaaccgta cttgcagata ccgtagggtt tattcgccac
    17101 ctgccgcacg atctggtggc ggcatttaaa gccacgttac aagagacgcg gcaagccaca
    17161 ttactgctgc acgtcattga tgcggcggat gtgcgtgtac aagaaaacat cgaagcggtg
    17221 aatacggttc ttgaagagat cgacgctcac gagatcccaa ccctgctggt gatgaacaag
    17281 atcgatatgc tggaagattt cgaaccgcgt attgatcggg acgaagagaa caaaccgatc
    17341 cgtgtctggc tttccgcaca gaccggagcg gggataccac agctttttca ggctttgacg
    17401 gagcggcttt ccggcgaggt ggcgcagcat acattgcgtc tgccaccgca ggaagggcgt
    17461 ctgaggagtc gtttttatca gcttcaggca atagaaaaag agtggatgga ggaggacggc
    17521 agcgtaagtc tgcaagttcg tatgccgatc gttgactggc gtcgcctctg taaacaagaa
    17581 ccggcgttga tcgattacct gatctaacgg cgtagcgtct gaagcgtgga gtcatatcct
    17641 ctggcgtcga aagacaacag ggatcaccgc ataacaaata tggagcacaa acatggcgtg
    17701 gaatcagccc ggtaataacg gacaagaccg cgacccgtgg ggaagcagca aacctggcgg
    17761 caactctgag ggaaatggaa acaaaggcgg tcgcgatcaa gggccacctg atttagatga
    17821 tatcttccgc aaactgagca aaaagctcgg tggtctgggc ggcggtaaag gcaccggatc
    17881 tggcggtggc agttcatcac aaggcccgcg cccgcagctt ggcggtcgtg tcgttaccat
    17941 cgcagcggca gcgattgtca ttatctgggc ggccagtggt ttctatacca ttaaagaagc
    18001 cgaacgcggc gtggtaacac gctttggtaa attcagccat ctggttgagc cgggtctgaa
    18061 ctggaaaccg acgtttatcg acgaagtcaa accggtgaac gtggaagccg tgcgtgaact
    18121 ggccgcttct ggtgtgatgc tgacgtcgga cgagaacgta gtgcgcgttg agatgaacgt
    18181 gcagtaccgc gtcaccaatc cggaaaaata tctgtatagc gtgaccagcc cggatgacag
    18241 cctgcgtcag gctaccgaca gcgccctgcg tggagttatc ggtaaataca ccatggaccg
    18301 cattctgacg gaaggtcgta ccgtgattcg tagcgatact cagcgcgaac tggaagagac
    18361 gattcgtccg tatgacatgg gtatcacgct gctggacgtc aacttccagg ctgctcgtcc
    18421 gccggaagaa gtaaaagcgg cgtttgacga tgcgattgcc gcgcgtgaaa acgaacagca
    18481 atacattcgt gaagcagaag cgtattccaa cgaagttcag ccgcgtgcga acggtcaggc
    18541 gcaacgtatc ctcgaagagg cgcgtgcgta caaggcccag accatcctgg aagctcaggg
    18601 tgaagtggcg cgctttgcta aacttctgcc ggaatataaa gccgcgccgg aaattactcg
    18661 cgagcgtctg tatatcgaga cgatggaaaa agtgttgggt aacacccgca aagtgctggt
    18721 taacgataaa ggtggcaacc tgatggttct gccgttagac cagatgctga aaggtggtaa
    18781 cgcccctgcg gcgaagagcg ataacggtgc cagcaatctg ctgcgtctgc cgccagcctc
    18841 ttcctccaca accagtggag caagcaacac gtcgtccacc agtcagggcg atattatgga
    18901 ccaacgccgc gccaacgcgc agcgtaacga ctaccagcgt cagggggaat aacgatgcgt
    18961 aagtcagtta tcgcgattat catcatcgtg ctggtagtgc tttacatgtc tgtctttgtc
    19021 gtcaaagaag gtgagcgcgg tattacgctg cgttttggta aggtactgcg tgacgatgac
    19081 aacaaacctt tggtttatga gcctggtctg catttcaaga taccgttcat tgaaacggtg
    19141 aaaatgctcg acgcacgtat tcagaccatg gacaaccagg ccgaccgctt tgtgaccaaa
    19201 gagaagaaag acctgatcgt cgactcttac atcaaatggc gcatcagcga tttcagccgt
    19261 tactacctgg caacgggtgg tggcgacatt tcgcaagcgg aagtgctgtt gaaacgtaag
    19321 ttctctgacc gtctgcgttc tgaaattggt cgcctggacg tgaaagatat cgtcaccgat
    19381 tcccgtggtc gtctgaccct cgaagtacgt gacgcgctga actccggttc tgcgggtaca
    19441 gaagatgaag ttactacccc ggcagcagat aacgccattg ccgaagcggc agagcgcgta
    19501 acggctgaga cgaagggcaa agttccggtc atcaacccga acagtatggc ggcgctgggt
    19561 attgaagttg tcgatgtgcg tatcaagcag atcaacctgc cgaccgaagt gtctgaagcg
    19621 atctacaacc gtatgcgcgc cgagcgtgaa gcggtagcgc gtcgtcaccg ttcacaaggt
    19681 caggaagaag cggaaaaact gcgcgcgact gccgactatg aagtgaccag aacgctggca
    19741 gaagctgagc gtcagggccg cattatgcgt ggtgaaggcg atgccgaagc agccaaactg
    19801 tttgctgatg cattcagtaa agatccggac ttctacgcat tcatccgtag cctgcgtgct
    19861 tatgagaaca gcttctctgg caatcaggac gtgatggtca tgagcccgga tagcgatttc
    19921 ttccgctaca tgaagacgcc gacttccgca acgcgttaat ataacgactg cggtacaggt
    19981 caataaagcc accgcatcct cagggatgtc ggtggttttc tttttctata aggataatga
    20041 atgaattcga caatctggct ggcgcttgcc ctggttttgg tactggaagg tttagggccg
    20101 atgctttacc cgaaggcatg gaagaagatg atctctgcga tgaccaattt gcccgataat
    20161 attttacgtc gttttggcgg tggacttgtg gttgcgggcg ttgtggtcta ctacatgttg
    20221 aggaaaacga ttggctgaac aaaaaacaga ctgatcgagg tcatttttga gtgcaaaaag
    20281 tgctgtaact ctgaaaaagc gatggtagaa tccattttta agcaaacggt gattttgaaa
    20341 aatgggtaac aacgtcgtcg tactgggcac ccaatggggt gacgaaggta aaggtaagat
    20401 cgtcgatctt ctgactgaac gggctaaata tgttgtacgc taccagggcg gtcacaacgc
    20461 aggccatact ctcgtaatca acggtgaaaa aaccgttctc catcttattc catcaggtat
    20521 tctccgcgag aatgtaacca gcatcatcgg taacggtgtt gtgctgtctc cggccgcgct
    20581 gatgaaagag atgaaagaac tggaagaccg tggcatcccc gttcgtgagc gtctgctgct
    20641 gtctgaagca tgtccgctga tccttgatta tcacgttgct ctggataacg cgcgtgagaa
    20701 agcgcgtggc gcgaaagcga tcggcaccac cggtcgtggt atcgggcctg cttatgaaga
    20761 taaagtggca cgtcgcggtc tgcgtgttgg cgaccttttc gacaaagaaa ccttcgctga
    20821 aaaactgaaa gaagtgatgg aatatcacaa cttccagttg gttaactact acaaagctga
    20881 agcggttgat taccagaaag ttctggatga tacgatggct gttgccgaca tcctgacttc
    20941 tatggtggtt gacgtttctg acctgctcga ccaggcgcgt cagcgtggcg atttcgtcat
    21001 gttcgaaggt gcgcagggta cgctgctgga tatcgaccac ggtacttatc cgtacgtaac
    21061 ttcttccaac accactgctg gtggcgtggc gaccggttcc ggcctgggcc cgcgttatgt
    21121 tgattacgtt ctgggtatcc tcaaagctta ctcaactcgt gtaggtgcag gtcctttccc
    21181 ggccgaactg tttgatgaaa ctggcgagtt cctctgcaag cagggtaacg aattcggtgc
    21241 aactacgggt cgtcgtcgtc gtaccggctg gctggacacc gttgccgttc gtcgtgcggt
    21301 acagctgaac tccctgtctg gcttctgcct gactaaactg gacgttctgg atggcctgaa
    21361 agaggttaaa ctctgcgtgg cttaccgtat gccggatggt cgcgaagtga ctaccactcc
    21421 gctggcagct gacgactgga aaggtgtaga gccgatttac gaaaccatgc cgggctggtc
    21481 tgaatccact ttcggcgtga aagatcgtag cggcctgccg caggcggcgc tgaactacat
    21541 caagcgtatt gaagagctga ccggtgtgcc gatcgatatc atctctaccg gtccggatcg
    21601 tactgaaacc atgattctgc gcgacccgtt cgacgcgtaa ttctggtacg cctggcagat
    21661 attttgcctg ccgggctaac agtgtgatac attgctgtgt cggttaagcc attacgctat
    21721 ccgacacagt gttaaatcct cgcttttttc cttccccgaa ctgaaataaa ttagcgacac
    21781 agcttgtggc tggtttatca tcaatataaa tgtatttttt cccgatttcc cttttgaggt
    21841 tgatgtgcag ttaacgagtt tcactgatta cggattacgt gcgctgatct acatggcgtc
    21901 attgccagaa gggcggatga ccagtatttc tgaagtgact gacgtctacg gcgtctcccg
    21961 taatcatatg gtcaaaataa tcaatcaact tagtcgtgcc ggctacgtga ctgctgttcg
    22021 tggaaaaaat ggcggcattc gcctgggtaa accggcgagt gcgatacgta ttggtgatgt
    22081 ggtgcgcgag ctggagccct tatcgctggt gaattgcagc agtgagtttt gccacattac
    22141 acctgcctgt cggttgaaac aggcgctttc taaggccgtg caaagttttc ttacggaact
    22201 ggataactac acgcttgccg atttggttga agagaatcaa ccgctttata aattattgct
    22261 ggtggagtga cgaaaatctt catcagagat gacaacggag gaaccgagat gtcacaagat
    22321 cctttccagg aacgcgaagc tgaaaaatac gcgaatccca tccctagtcg ggaatttatc
    22381 ctcgaacatt taaccaaacg tgaaaaaccg gccagccgtg atgagctggc ggtagaactg
    22441 cacattgaag gcgaagagca gcttgaaggc ctgcgtcgcc gcctgcgcgc gatggagcgc
    22501 gatggtcaac tggtcttcac tcgtcgtcag tgctatgcgc tgccggaacg cctcgacctg
    22561 gtgaaaggta ccgttattgg tcaccgtgat ggctacggct ttctgcgggt tgaagggcgt
    22621 aaagatgatt tgtatctctc cagcgagcag atgaaaacct gcattcatgg cgatcaggtg
    22681 ctggcgcagc cgctgggtgc tgaccgtaaa ggtcgtcgtg aagcgcgtat tgtccgcgta
    22741 ctggtgccaa aaaccagcca gattgttggt cgctacttta ccgaagcggg cgtcggcttt
    22801 gtggttcctg acgatagccg tctgagcttc gatatcttaa tcccgcccga tcagatcatg
    22861 ggcgcgcgga tgggctttgt ggtcgtagtc gaactgactc agcgtccgac tcgccgcacc
    22921 aaagcggtgg gtaaaatcgt cgaagtgctg ggcgacaata tgggcaccgg catggcggtt
    22981 gatatcgctc tgcgtaccca tgaaattccg tatatctggc cgcaggctgt tgagcaacag
    23041 gttgcggggc tgaaagaaga agtgccggaa gaagcaaaag cgggccgtgt cgatttgcgc
    23101 gatttaccgc tggtcaccat tgatggcgaa gacgcccgtg actttgacga tgcagtttac
    23161 tgcgagaaaa aacgcggcgg cggctggcgt ttatgggtcg cgattgccga cgtcagctac
    23221 tatgtgcgtc cgccaacgcc gctggacaga gaagcgcgta accgtggcac gtcggtgtac
    23281 ttcccttcgc aggttatccc gatgctgccg gaagtgctct ctaacggcct gtgttcgctc
    23341 aacccgcagg tagaccgcct gtgtatggtg tgcgagatga cggtttcgtc gaaaggccgc
    23401 ctgacgggct acaaattcta cgaagcggtg atgagctctc acgcgcgtct gacctacacc
    23461 aaagtctggc atattctgca gggcgatcag gatctgcgcg agcagtacgc cccgctggtt
    23521 aagcatctcg aagagttgca taacctctat aaagtgctgg ataaagcccg tgaagaacgc
    23581 ggtgggatct catttgagag cgaagaagcg aagttcattt tcaacgctga acgccgtatt
    23641 gaacgtatcg aacagaccca gcgtaacgac gcgcacaaat taattgaaga gtgcatgatt
    23701 ctggcgaata tctcggcggc gcgtttcgtt gagaaagcga aagaaccggc actgttccgt
    23761 attcacgaca agccgagcac cgaagcgatt acctctttcc gttcagtgct ggcggagctg
    23821 gggctggagc tgccgggtgg taacaagccg gaaccgcgtg actacgcgga actgctggag
    23881 tcggttgccg accgtcctga tgcagaaatg ctgcaaacca tgctgctacg ctcgatgaaa
    23941 caggcgattt acgatccaga aaaccgtggt cacttcggtc tggcattgca gtcctatgcg
    24001 cactttactt cgccgattcg tcgttatcct gacctgacgc tgcaccgcgc cattaaatat
    24061 ctgctggcga aagagcaggg gcatcagggc aacaccactg aaaccggcgg ctaccattat
    24121 tcgatggaag agatgttgca actgggtcag cactgttcga tggcggaacg tcgtgccgac
    24181 gaagcaacgc gcgatgtcgc tgactggctg aagtgtgact tcatgctcga ccaggtaggt
    24241 aacgtcttta aaggcgtaat ttccagcgtc actggctttg gcttcttcgt ccgtctggac
    24301 gacttgttca ttgatggtct ggtccatgtc tcttcgctgg acaatgacta ctatcgcttt
    24361 gaccaggtag ggcaacgcct gatgggggaa tccagcggcc agacttatcg cctgggcgat
    24421 cgcgtggaag ttcgcgtcga agcggttaat atggacgagc gcaaaatcga ctttagtctg
    24481 atctccagtg aacgcgcacc gcgcaacgtc ggtaaaacgg cgcgcgagaa agcgaaaaaa
    24541 ggcgatgcag gcaaaaaagg cggcaagcgt tgtcaggtcg gtaaaaaggt aaactttgag
    24601 ccagacagcg ccttccgcgg tgagaaaaaa acgaagccga aagcggcgaa gaaagacgcg
    24661 agaaaagcga aaaagccatc ggcgaaaacg cagaaaatag ccgcagcgac caaagcgaag
    24721 cgtgcggcga agaaaaaagt ggcagagtga tcaataccct ctttaaaaga agagggttag
    24781 attgctgaca aaatgcgctt tgttcatgcc ggatgcggcg tgaacgcctt atccggccta
    24841 cataatcacg caaattcaat atattgcaga gatcatgtag gccggataaa cgtagtgcat
    24901 caggcaaaac gtaaacaacg agtacattaa tgagcgaaat gatttacggc atccacgcag
    24961 tgcaggccct gctggagcgc gcccctgaac gttttcagga agtctttatt ttaaaaggcc
    25021 gtgaagataa acgtctgtta ccgctgattc acgcccttga gtctcagggc gtggttatcc
    25081 agttggcaaa ccgccaatat ctcgacgaga aaagcgacgg tgccgtgcat cagggcatta
    25141 tcgcccgcgt gaagccagga cgtcagtatc aggaaaacga tctgccggat ctgatcgctt
    25201 cgctcgatca accgttcctg ctgatcctcg acggcgtaac cgatccgcac aacctcggcg
    25261 cgtgcctgcg tagtgcggac gccgcaggcg ttcatgcggt gattgtgccg aaagatcgct
    25321 ccgcacagct caacgctacg gcgaaaaaag tagcctgcgg cgcggcagaa agcgttccgc
    25381 tgattcgggt gactaacctt gcgcgcacca tgcgtatgtt gcaggaagag aatatctgga
    25441 tcgtcggtac ggcaggcgag gcggatcata cactctatca gagcaaaatg accggacgcc
    25501 tggcgctggt gatgggcgcg gaaggtgaag gtatgcgtcg cctgacccgt gaacattgcg
    25561 atgagttgat cagcatccca atggcaggaa gtgtttcttc cctgaacgtt tcggttgcaa
    25621 ccggaatttg cttatttgaa gcggttcgtc agcgcagcta gttcctctga attatgaaaa
    25681 gccatccaga tttggatggt tt
//
LOCUS       DAEBXN010000055        25659 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.055, whole genome shotgun sequence.
ACCESSION   DAEBXN010000055 DAEBXN010000000
VERSION     DAEBXN010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25659
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.055"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(<1..1358)
                     /gene="yeaG"
                     /locus_tag="KJL99_002326"
     CDS             complement(<1..1358)
                     /gene="yeaG"
                     /locus_tag="KJL99_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HBD4437031.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQDPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNKSEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQ"
     gene            1794..2540
                     /gene="mipA"
                     /locus_tag="KJL99_002327"
     CDS             1794..2540
                     /gene="mipA"
                     /locus_tag="KJL99_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HBD4437032.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            2630..3391
                     /locus_tag="KJL99_002328"
     CDS             2630..3391
                     /locus_tag="KJL99_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBD4437033.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLGYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHV
                     QQHAAALEVELSSAELAMLDKAYPAPKGKTALDMV"
     gene            complement(3442..4326)
                     /locus_tag="KJL99_002329"
     CDS             complement(3442..4326)
                     /locus_tag="KJL99_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HBD4437034.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(4410..5405)
                     /gene="gapA"
                     /locus_tag="KJL99_002330"
     CDS             complement(4410..5405)
                     /gene="gapA"
                     /locus_tag="KJL99_002330"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBD4437035.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            5747..6160
                     /gene="msrB"
                     /locus_tag="KJL99_002331"
     CDS             5747..6160
                     /gene="msrB"
                     /locus_tag="KJL99_002331"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HBD4437036.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            6202..6474
                     /locus_tag="KJL99_002332"
     CDS             6202..6474
                     /locus_tag="KJL99_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HBD4437037.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            6844..7920
                     /locus_tag="KJL99_002333"
     CDS             6844..7920
                     /locus_tag="KJL99_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HBD4437038.1"
                     /translation="MKALARFGKAFGGYKMIHVPQPICGPEDVVTEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            7947..9326
                     /locus_tag="KJL99_002334"
     CDS             7947..9326
                     /locus_tag="KJL99_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD4437039.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMDVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLITM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSNIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            9343..10388
                     /locus_tag="KJL99_002335"
                     /pseudo
     CDS             9343..10388
                     /locus_tag="KJL99_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
     gene            10409..11245
                     /locus_tag="KJL99_002336"
     CDS             10409..11245
                     /locus_tag="KJL99_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HBD4437040.1"
                     /translation="MLADIRYWENDATNKYYAIAHFNVWNAEMLMGVKDAAEEAKSPV
                     IISFSTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSIEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIELFGSDGK
                     AE"
     gene            11250..12197
                     /locus_tag="KJL99_002337"
     CDS             11250..12197
                     /locus_tag="KJL99_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HBD4437041.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMSTGGDA
                     INEATIISRLGHRTALMSRIGKDASGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFPQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNETLDDICEALSYVDYLFPNFAEEKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            12207..13187
                     /gene="ydjG"
                     /locus_tag="KJL99_002338"
     CDS             12207..13187
                     /gene="ydjG"
                     /locus_tag="KJL99_002338"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HBD4437042.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTALGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            13324..14082
                     /locus_tag="KJL99_002339"
     CDS             13324..14082
                     /locus_tag="KJL99_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBD4437043.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAVLTENIHFYKRASSFYEEKLLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            14200..15558
                     /locus_tag="KJL99_002340"
     CDS             14200..15558
                     /locus_tag="KJL99_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD4437044.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLYAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLEGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFRLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSSQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(15651..16292)
                     /gene="pncA"
                     /locus_tag="KJL99_002341"
     CDS             complement(15651..16292)
                     /gene="pncA"
                     /locus_tag="KJL99_002341"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HBD4437045.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLGGLRQTFWPEHCVQNSEGAQLHPLLNQ
                     KEIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(16303..17319)
                     /gene="ansA"
                     /locus_tag="KJL99_002342"
     CDS             complement(16303..17319)
                     /gene="ansA"
                     /locus_tag="KJL99_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HBD4437046.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAPNYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMSGYATGNALAHAGVIDGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(17486..19342)
                     /gene="sppA"
                     /locus_tag="KJL99_002343"
     CDS             complement(17486..19342)
                     /gene="sppA"
                     /locus_tag="KJL99_002343"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HBD4437047.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKSVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            19503..20054
                     /locus_tag="KJL99_002344"
     CDS             19503..20054
                     /locus_tag="KJL99_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HBD4437048.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGCERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCKENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            20171..21214
                     /gene="selD"
                     /locus_tag="KJL99_002345"
     CDS             20171..21214
                     /gene="selD"
                     /locus_tag="KJL99_002345"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HBD4437049.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            21219..23180
                     /gene="topB"
                     /locus_tag="KJL99_002346"
     CDS             21219..23180
                     /gene="topB"
                     /locus_tag="KJL99_002346"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBD4437050.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKVLRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            23307..24347
                     /locus_tag="KJL99_002347"
     CDS             23307..24347
                     /locus_tag="KJL99_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HBD4437051.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWGIFNSNFERVIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(24464..>25659)
                     /gene="gdhA"
                     /locus_tag="KJL99_002348"
     CDS             complement(24464..>25659)
                     /gene="gdhA"
                     /locus_tag="KJL99_002348"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HBD4437052.1"
                     /translation="LERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGG
                     MRFHPSVNLSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGKVMRFCQTLMT
                     ELYRHLGADTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEA
                     TGYGLVYFTEAMLKRHGMGFEGMSVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTV
                     VDESGFTKEKLARLIEIKASRDGRAADYAKEFGLVYLEGQQPWSVPVDIALPCATQNE
                     LDVDAAHQLIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEM
                     AQNAARLGWKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAML
                     AQGVI"
BASE COUNT         6517 a         5971 c         6624 g         6547 t
ORIGIN      
        1 tgctgttcca ggacgtagaa cagatggacc gggtttgccg ccacttctac atgatcgaag
       61 ttgaacacgc gggagaggat cttaaacgca aaacgcgtcg acagaccgtt catcccttca
      121 tcgacaccgg cgtagtcacg atattcctga tacgacttgg ctttcggatc ggtgtctttc
      181 agactttcgc catcataaac ccgcatcttt gaataaatgc tggagttttc tggctctttc
      241 aggcgcgaaa gaatggaaaa acgtgacagt gtttcgagcg tgccaggggc gcatggggcg
      301 tgagtcaatt cactgtgatt aagcaatttc tcgtagattt tgatctcttc ggaaatgcgc
      361 aagcaatacg gcaccttcac gatgtaaaca cggtcgagga aggcttcgtt gtttttgtta
      421 ttacggaaag tgacccattc ggacttgttc gagtgggcga gaataatccc gttgaacggc
      481 agggcggaga taccttccgt cccgttgtag ttaccttcct gagtggcggt taacaaggga
      541 tgcagcactt ttatcggtgc tttaaacatc tcaacgaatt ccatgatccc ctgattggcg
      601 cggcacagcg caccggaata gccgtaggcg tccgggtcat tctgcgcgta gtgttcgagt
      661 ttacgaatat cgactttccc aaccagcgcg gagatgtcct ggttgttctc atcaccgggt
      721 tccgttttgg cgatagcaat ttgttgcaga attgacggcc agaccttcac tacccggaac
      781 ttagtgatat cgccaccaaa ttcatgcagg cgttttgccg cccatggcga catgatggtg
      841 ccgagataac ggcgaggaat gccatactct ttttccagaa tctgagcatc ttcctgcgga
      901 ttgaaaagac agaacggatg atcgttgacc gggctacgct caccgttggc gctcaataca
      961 taaatcggta cgagctgcat taatgatttc agtcgctcag caagcgatga tttaccccca
     1021 cccacaggcc ccagcagata caggatttgt ttcttctctt ccagcccctg agccgcgtgt
     1081 ttcagataag agacaatctg ttcaatcgcg tcttccatgc cgtaaaactc ttcaaatgcc
     1141 ggataacgtg caatgacccg gttagaaaag agtcgagaaa gtctggggtc ctgggctgta
     1201 tcgaccatga caggctcacc gatagccatc aatagccgct cagccgcgtt ggcataagca
     1261 ctgcgatctt gccgacaagt ggtaagaaac tcctgcagtg tgaactcttc gtccttggca
     1321 gcttcatatc gctggcgata gtgatcgaat atattcatgg tatgccgtcc tttcgttttt
     1381 tagcacaggt taagagccgt tcatatgaat agtagaggct cccggaagag gtaaactgaa
     1441 cgacgtctct tgctaaaaca cagcaactct catgccattt tatcgacgat gagaacaacg
     1501 tgcccggtga tacaccttca aaattaagcg tagatggcaa atggaaaact tgcctgcacg
     1561 gctttcttaa tttcaatggc atatcaacaa ctcatccata aattttgcat aattaatgta
     1621 aagaccaggc tcgccagtaa cgctaaatcc atttggctgt aagcgcggtg tcatccgcgg
     1681 caggaaaatt aaacagttac tttaaaaaat gaaaacgtaa aaaggttggg tttcgatgta
     1741 ttgacgggta aactttgtcg cccgctaaac atttgtttaa ggaatgatta attgtgacca
     1801 aactcaaact tctggcactt ggagtgctta tcgcaacgtc tgcaggcgta gcgcacgctg
     1861 aaggtaaatt ttccctgggc gcaggcgtag gtgtcgttga gcacccatat aaagattacg
     1921 ataccgatgt ttacccagta ccggtaatca actatgaagg cgataacttc tggttccgtg
     1981 gcttaggtgg tggttactac ctgtggaatg acgcaacgga taaactttca attaccgctt
     2041 actggtcgcc gctttacttc aaagcgaaag acagtggcga tcaccaaatg cgtcacctgg
     2101 atgaccgtaa gagcaccatg atggctggtc tgtcttatgc tcactttacc cagtacggtt
     2161 acctgcgtac caccctggct ggcgataccc tggataacag caacggcatt gtctgggata
     2221 tggcctggtt gtatcgttac accaacggtg gcctgaccgt gactccgggt attggtgtgc
     2281 agtggaacag cgaaaaccag aacgaatact attatggcgt atcgcgcaaa gagtccgctc
     2341 gcagcggtct gcgtggctat aacccgaatg acagctggag cccttacctt gagctgagcg
     2401 ccagctacaa cttcctcggc gactggagtg tttacggtac cgcgcgctac acccgtctgt
     2461 ctgatgaagt tactgacagc ccgatggtgg ataaatcctg gactggcctg atttctaccg
     2521 ggatcaccta caaattctga taatgcatct ttccagggca ttttgtgcat aaaaacaggg
     2581 cgtcacgccc tgttttgcat tacggtggtg caatacctgg gaggaaacaa tgcaacaaaa
     2641 aatgattcaa tttagtggcg atgtctcact gccagccata gggcagggaa catggtatat
     2701 gggcgaagat gccagtcagc gcaaaacaga agttgctgca ctacgcgcag gcattgaact
     2761 cggtttaacc ctcattgata ccgccgaaat gtatgccgat ggcggtgccg aaaaggtggt
     2821 tggggaagca ttaaccggtc tgcgagagaa ggtctttctc gtctctaaag tctatccgtg
     2881 gaatgctggc gggcaaaaag cgataaatgc atgcgaagcc agtttacgcc gtctcaatac
     2941 tgactatctc gatctctact tattacactg gtctggcagt ttcgcttttg aagagactgt
     3001 tgctgcgatg gaaaaattga tcgcccaggg aaaaatccgt cgctggggcg tttctaacct
     3061 tggttatgcc gatatgcagg aactctggca gctgccgggg ggaaatcagt gtgccactaa
     3121 tcaggtgctt taccatctcg gttcacgagg gattgagtac gatctactcc cctggtgcca
     3181 gcaacagcag atgccggtga tggcttacag tccattagcc caggccgggc ggttgcgcaa
     3241 tggactgtta aaaaacgcgg tagtcaacga aattgcacat gtccaacaac atgcagctgc
     3301 gcttgaggtc gaactttcgt cagcggaatt agctatgctg gataaggcat atccggcacc
     3361 aaaaggaaaa actgcgctgg atatggtgtg atgttgatac gtgccggatg catcgcaccc
     3421 ggcacacaac attaacgcaa attaacgttt ggcaacgcga atggattgcg ccagatgtgc
     3481 aggtttctct ttggtgactt tttgcgtttc tgaagcgtaa gccgtttcta cacaaacaaa
     3541 ggtcttgtag ccatcgtccg gcatgtcgcc catgctaact gaaagcgccg gtcccgggtt
     3601 ccagccgaca acgttcagat gatgctggtg gcctacggcg ataatacgat tcagcgcttc
     3661 atcattaatc acgctgcaat cttgcggatt cagatacacg cgatcggtac ggtcagggaa
     3721 ggtctgaata ccgtcggtca gtacatcttc tttcgcgtca ttcactttat caatgaagcg
     3781 atcgcccagc ccactgacgc ttaccttagc gatatcaccc acgttaaagt aggtatgcag
     3841 ggcagaggtg gtttcaaatt cgccatgtga ttcaagatcg atttcacagg ttttacccac
     3901 gcggaaatgc gctaacagcg taaagtcgtg cggccagaat tttttcgtct cttcgctttg
     3961 tgtcaattca aaagtcagcg ctacgccatc agcatcttca tgatgtgatt tcagcgtcca
     4021 cggcaggttg cgggcaaaac cgtgcgcagg cagaccttgt tgtgccgccg gaccaaacca
     4081 cggccagcaa accggtacgc caccgcgaat agcgacgcca tttttgaacg gtgtgttgtt
     4141 gctcaaccac agaacttctt cttcacccgc aggtttccac gagagaaggt gtgcgccctg
     4201 taatgcaaaa gaggctttta cctggggatg atcgaccaca atgaggtcca gttcatccag
     4261 tttacgacgg gagaggacag gggagatttg ttcgatgacc ggaagggcaa aaattttctt
     4321 aatcatgacg cagtccttta acttcatttt atcaggtaaa aaaaagagcg accgaagtcg
     4381 ctctttttag atcacagtgt catctcaact tatttggaga tgtgagcgat caggtccaga
     4441 actttgttgg agtaaccggt ttcgttgtcg taccaggata ccagtttcac gaagttgtcg
     4501 ttcagagcga taccagcttt agcatcgaac acggaagtgc aaacttcgcc gttgaaatcg
     4561 gtagatacta cgtcatcttc ggtgtagccc agaacgcctt tcatttcgcc ttcagcagca
     4621 gctttaacgg cagctttgat ctgctcgtaa gttgcagctt tttccagacg aacggtcagg
     4681 tcaactacgg atacgttcgg ggtcggaacg cggaacgcca taccagtcag tttgccattc
     4741 agttctggca gtactttacc tacagcttta gcagcaccgg tagaggacgg gatgatgttc
     4801 tgggaagcgc cgcggccgcc gcgccagtct ttgtgagacg ggccatcaac ggttttctga
     4861 gtagcggtag tagcgtgaac ggtggtcatc agaccttcga tgatgccgaa gttatcgttg
     4921 ataactttag ccagcggagc caggcagttg gtggtgcagg aagcgttgga aacgatgtcc
     4981 tggccagcat atttgtcgaa gttagcgcct ttaacgaaca tcggagtgtt gtctttagac
     5041 ggaccagtca taaccacttt cttcgcacca gcggtgatgt gtttacgagc agtttcgtca
     5101 gtcaggaaca gaccagttgc ttcagcgaca acgtcaacac ccacttcgtc ccatttcagg
     5161 ttagccggat cacgttcagc ggtaacacgg atttttttac cgttaacgat cagatgaccg
     5221 tctttcactt caacggtacc gtcgaaacgg ccgtgagtgg agtcatattt cagcatgtat
     5281 gccatgtaat cagcgtctaa caggtcgttg attgcaacga tctcgatgtc agaacgtttc
     5341 tgagcagcac ggaaaacaat gcgaccgata cggccaaaac cgttgatacc tactttgata
     5401 gtcatatatt ccaccagcta tttgttagtg aataaaaggt tgcctgtaaa attacaaaaa
     5461 ccttacgcag cgtcaagcgg aatcgtgtca atcattgcga caaatcaatc ctgtgcctaa
     5521 gcattacgcg actgactcgc ctcacttttc ctttgggctt gagaccacat ggggtcggcg
     5581 ccccgaattt taaagggcaa ttacgataaa aatgtgattt tcatcacgat ttaggtgcag
     5641 ccatttcgtc gagatcaagt ttaggacaaa attttgccag ttggtgttaa tgttttgtta
     5701 gaatcagtca cgtaatgtga gcacgattaa agtgagatgt gagtaaatgg cgaataaacc
     5761 ttcggcagaa gaactgaaaa aaaatttgtc cgagatgcag ttttacgtga cgcagaatca
     5821 tgggacagaa ccgccattta cgggtcgttt actgcataac aagcgtgacg gcgtatatca
     5881 ctgtttgatc tgcgatgccc cgctgtttca ttccgaaacc aagtatgatt ccggctgtgg
     5941 ctggcccagt ttctacgaac cggtgagtga agaatccatt cgttatatca aagacttgtc
     6001 acatggaatg cagcgcatag aaattcgttg cggtaactgt gatgcccatc tggggcatgt
     6061 ctttcccgac gggccgcaac caacaggcga acgttattgt gttaactctg cttctttacg
     6121 cttcactgat ggcgaaaacg gcgaagaaat caacggttga aaaaacgatt cagcaactta
     6181 ttccacagga gcggaaaaat tatgaatctt gatgacatta tcaacagcat gacgcctgag
     6241 gtataccagc gtttgtcgac cgccgttgaa ctggggaaat ggcctgatgg cgttgcgttg
     6301 accgaggaac aaaaagagaa ttgcctgcaa ctggtgatgc tgtggcaagc ccgccataat
     6361 accgaagcac agcatatgac aattgacaca aatggtcaga tggtcatgaa gagcaaacag
     6421 cagttaaaag aagattttgg tatctcagca aagcctattg ccatgtttaa gtaagtggtt
     6481 catccccttt ttgccgcggc tatgaagggg atagccaaca atgaccccac gactcgtctc
     6541 aattatctcg acccgacctg gctaagtccc agtccgatct cttgcagtcg cgttcacgcc
     6601 tgaatgactt ctcctgaact taagtctcag acctatttgg ccggtaatcc ctctcgaatt
     6661 tgctcttttt gcgtcccgcc ttccaccaac ctgtaacaga cccagccatt ttctgcgtaa
     6721 ttgtgatttg agttgtaaaa tgcaggtcat tatctgtgtt gtgcagattt aatattgacc
     6781 tcaaaaatca tgtgggtaag tatgatggcc atctaagcaa aacaaaacac agaggatgac
     6841 gcaatgaaag cactggctcg gtttggcaag gcctttggcg gctacaagat gattcatgtc
     6901 ccacaaccca tttgtggccc ggaagacgta gtgactgaaa ttaaagccgc ggcaatctgc
     6961 ggcgcagaca tgaagcacta caatgtcgat agcggttctg atgagtttaa ctctatccgc
     7021 ggccatgagt tcgcaggttg tattgcgcag gttggtgaaa aagtcaaaga ctggaaagtg
     7081 gggcaacgcg tcgtgtcgga taacagcggt cacgtttgcg gcgtttgtcc ggcctgtgaa
     7141 cagggtgatt ttctgtgttg tacagaaaaa gtaaaccttg gtctggataa caatacctgg
     7201 ggcggtggtt tttccaaata ttgtctggtt cctggtgaaa ttctcaaaat tcatcgtcat
     7261 gcgttgtggg aaatccctga tggtgttgat tatgaggacg cagccgtact tgaccctatc
     7321 tgtaatgcct ataaatccat cgcgcagcaa tcgaaattcc ttcctggtca ggatgttgtc
     7381 gtcattggca ctggcccact cgggctgttt tccgtacaaa tggcgcggat tatgggggcg
     7441 gtaaatatcg tcgtcgttgg tctgcaagaa gatgtggcgg tccgcttccc ggttgcaaaa
     7501 gaactggggg cgacggcagt agtaaatggt tctaccgaag atgtggtggc gcgctgccag
     7561 caaatttgtg gcaaagataa tctgggactg gtgattgaat gctccggtgc caatatcgca
     7621 ctgaaacaag ccatcgaaat gctccgcccg aatggggaag tggtacgcgt tggaatgggc
     7681 ttcaaacctc ttgatttctc gattaatgac attaccgcct ggaataaaag catcattggg
     7741 catatggcct atgactccac ctcatggcgt aacgctatcc ggctattagc cagcggcgct
     7801 atcaaagtca aaccgatgat cacgcatcgt atcggcctgt cgcaatggcg cgaagggttt
     7861 gatgcgatgg tcgataaaac cgcaatcaaa gtgatcatga cttacgactt tgatgaataa
     7921 ctactctcct gaaggaacgt tatcgcatgg aacagataac aaaaccgcat tgtggtgcac
     7981 ggctggatcg cttaccggat tgccgctggc attcgtcaat gtttgccatc gtcgcgtttg
     8041 gcttgcttgt ctgctggagt aatgccgttg gtggcttgat cctcgcgcag ctgaaagcgt
     8101 tgggctggac agataattcc actacagcca cattctcagc aatcacgacc gccggaatgt
     8161 ttctcggtgc gctggtgggc ggcatcattg gcgacaaaac cggtcgcaga aatgcgttca
     8221 tcctctatga ggccattcat attgcctcga tggttgtcgg tgctttctca ccgaatatgg
     8281 attttcttat tgcctgccgt tttgtgatgg acgttgggct gggagcttta ctggttacac
     8341 tgtttgctgg tttcaccgaa tatatgcccg gtagaaatcg cggaacgtgg tccagtcggg
     8401 tttcctttat tggcaactgg tcatatccgc tctgctcact gattgcgatg ggactcacgc
     8461 cgctgattag tgcagagtgg aactggcggg tacaactgct tattcctgca atattgtcgc
     8521 ttatcgctac ggcgctggcc tggcgctact ttcctgaatc cccgcgctgg ctggaatcgc
     8581 gcggacggta tcaggaagcc gagaaagtca tgcggagtat agaagaaggc gtcatacgcc
     8641 agacgggtaa acctttgccg cctgtggtta ttgctgatga cggtaaagcg ccacaagcgg
     8701 tgccgtattc agccttactg acaggagtat tactgaaacg cgtgatatta ggctcttgtg
     8761 tgctgattac catgaacgtt gtgcagtaca cactaattaa ctggttgcca acaatattca
     8821 tgacccaggg gattaattta aaagactcga ttgttttaaa taccatgagt atgtttggcg
     8881 cgccatttgg tatttttatt gccatgctgg tgatggataa aattccgcgt aaaacaatgg
     8941 gtgtggggct attaatcctg attgcggtgc tcggatatat ctattcactg caaaccagta
     9001 tgttgctcat aacgctgatt ggtttcttcc tgattacttt cgtctatatg tacgtttgct
     9061 atgcctcggc agtgtatgtc cctgaaatct ggccgacaga ggccaaactc cgtggctccg
     9121 gtctggcgaa tgcggtaggg cgaatcagtg gtattgccgc accttatgcc gttgcagtgc
     9181 tgctcagtag ttatggtgta acgggagtct ttattcttct gggggctgtt tcaaatattg
     9241 tcgcaattgc tatcgccacc attggaattg aaaccaaagg tgtctccgtt gaaagtttaa
     9301 gtattgatgc agtagccaat aaataatacg ggtggattca aaatgaaaaa ttcaaaagca
     9361 atattgcagg tgccgggcac aatgaaaatt atttcagcag aaataccagt gcctaaagaa
     9421 gatgaagttt tgattaaagt agaatatgtc ggtatttgtg gttcagatgt acatggtttt
     9481 gaatcaggcc cgtttattcc gcctaaagac ccaaatcaag aaattggcct gggtcatgaa
     9541 tgcgccggga cggttgtggc tgtgggaagc cgcgtgcgca aatttaaacc gggggatcgg
     9601 gtaaatatcg aacctggcgt tccttgcggt cactgtcgtt actgtctgga aggcaaatat
     9661 aacatctgcc cggacgttga ttttatggcg acacaaccca actaccgcgg cgcattaacg
     9721 cactatctgt gtcatccgga gagctttact tacaaactgc ccgacaatat ggacacgatg
     9781 gaaggggcgc tggtggagcc tgccgcagtc gggatgcatg ccgcgatgct ggcagatgtt
     9841 aaaccgggta agaagataat tattctggga gcaggttgta ttggtttgat gacgttgcaa
     9901 gcgtgcaaat gcctgggcgc aacggaaatt gccgtcgttg atgtactgga aaaacgtctg
     9961 gcaatagggg aacagcctgg tgcgacagtg gttattaatg gcgcaaaaga ggacactatt
    10021 gcacgctgtc agcaatttac cgaagacatg ggcgcagata ttgttttcga aacagcgggt
    10081 tctgcggtca ccgttaaaca ggcaccttta atctggtaat gcgcggcggt aaaattatga
    10141 ttgttggtac tgtacccggc gattcggcaa tcaatttcct caaaatcaat cgcgaagtca
    10201 ctatccagac ggtattccgc tatgccaatc gttatccggt cacgattgaa gctatttctt
    10261 cagggcgatt cgatgtgaaa tcgatggtga cgcatattta cgattatcgg gatgtacaac
    10321 aggcatttga agagtcagtt aacaacaaac gcgacattat taaaggcgtt attaaaatta
    10381 gcgattaatt aatacgggag aacagaacat gctcgcagat atcagatatt gggaaaacga
    10441 tgccacaaat aaatattatg caattgccca ttttaacgta tggaatgcag aaatgttgat
    10501 gggcgttaaa gacgcagccg aagaagcgaa atccccggtt attatttctt ttagtacagg
    10561 ttttgtcggt aacacctcat ttgaagattt ctctcacatg atggtatcaa tggcacaaaa
    10621 agcaacggtg ccggtaataa ctcattggga tcatggtcgg agtattgaga ttattcataa
    10681 cgcctggact catggtatga attcattaat gcgtgatgct tctgcatttg atttcgaaga
    10741 aaatattcgt ttaaccaaag aggctgttga tttctttcat ccgctgggta ttccggtaga
    10801 ggcggaatta gggcatgtcg gtaatgaaac cgtttatgag gaagcgctgg cgggctatca
    10861 ctataccgat cctgaccagg cggctgaatt tgttgaacgt acgggctgtg actcattggc
    10921 tgtcgccatc ggcaaccagc atggggttta tacgtcagag ccgcaattga actttgaggt
    10981 cgtcaaacgc gtacgcgatg ctgtttctgt tccgctggtt ttgcatggcg catcggggat
    11041 cagtgatgcc gacattaaaa ctgcaatttc gttgggtatt gcgaaaatca acattcatac
    11101 ggagctctgt caggccgcaa tggtagcagt gaaagaaaac caggatcagc ctttcctgca
    11161 tttagaacgt gaagtacgta aagctgtaaa agaacgggca ttggaaaaaa ttgaactgtt
    11221 cggttctgat ggcaaagcgg aataatgata tggataatct cgacgttatt tgtataggtg
    11281 ccgctattgt tgatattcca ttacaaccgg tcagtaaaaa tatctttgat gtagattctt
    11341 atcctcttga aagaatcgca atgagcaccg ggggcgatgc aataaatgaa gcaacaatta
    11401 tttctcgtct ggggcatcgt accgcattaa tgagccgcat tggtaaagat gcctcagggc
    11461 aatttattct cgaccattgt cgcaaagaga atattgatat tcaaagcctg aaacaggacg
    11521 tcagcataga tacatctatt aacgttggac tggtgacgga ggatggcgag cggacgtttg
    11581 tcacgaaccg taacggcagt ttgtggaaat taaatattga cgatgttgat tttgcgcggt
    11641 ttcctcaggc gaaattatta tcactggcca gtattttcaa cagtcctcta ttggatggta
    11701 aagcgctaac agaaattttt acgcaagcga aagcccggca gatgatcatt tgtgccgata
    11761 tgatcaaacc gcggttgaat gaaacgctgg atgatatttg cgaagcattg agttatgtcg
    11821 attatctgtt tccaaatttt gccgaggaaa aattactcac agggaaagag acactggatg
    11881 aaattgctga ctgctttctt gcgtgcggcg taaaaacggt ggtgattaaa acgggtaaag
    11941 acggctgctt tatcaagcgt ggtgacatga cgatgaaggt gccggcggtc gcaggaataa
    12001 ccgccatcga caccattggc gcgggcgata actttgcttc aggttttatt gcggcactgt
    12061 tagaaggcaa aaatctgcgt gaatgcgcac gctttgccaa tgcaacggcg gctatctcgg
    12121 ttctaagcgt cggtgccacc accggcgtaa aaaacagaaa gctggtcgaa caattgctgg
    12181 aagaatacga aggataatga aggcaaatga aaaagatacc tttaggcacg acggatataa
    12241 cgctttcgcg aatggggttg gggacatggg ccattggcgg cggtcctgca tggaatggcg
    12301 atctcgatcg gcaaatatgt attgatacga ttcttgaagc ccatcgttgt ggcattaatc
    12361 tgattgatac tgcgctagga tataactttg gcaatagtga agttatcgtc ggtcaggcgt
    12421 taaaaaaact gccccgtgaa caggttgtag tagaaaccaa atgcggcatt gtctgggaac
    12481 gaaaaggaag tttattcaac aaagttggcg atcggcagtt gtataaaaac ctttccccgg
    12541 aatctatccg cgaagaggta gaagccagct tgcaacgtct gggtattgat tacatcgata
    12601 tctatatgac gcactggcag tcggtgccgc cattttttac gccgatagct gaaactgtcg
    12661 cagtgcttaa tgagttaaag gctgaaggga aaattcgcgc tataggcgct gctaacgtcg
    12721 atgctgacca tatccgcgag tatctgcaat atggtgaact ggatattatt caggcgaaat
    12781 acagtattct cgaccgggca atggaaaacg aactgctgcc gctatgtcgt gataatggca
    12841 ttgtggttca ggtttattcc ccgctagagc agggattgtt gaccggcact atcactcgtg
    12901 attacgttcc gggcggcgct cgggcaaata aagtctggtt ccagcgtgaa aacatgctga
    12961 aagtgattga tatgcttgaa cagtggcagc cactttgtgc tcgttatcag tgcacaattc
    13021 ccactctggc actggcgtgg atattaaaac agagtgattt aatctccatt cttagtgggg
    13081 ctactgcacc ggaacaggta cgcgaaaatg tcgcggcact gaatatcaac ttatcggatg
    13141 cagacgcaac attgatgagg gaaatggcag aggccctgga gcgttaaata ctcaacacga
    13201 ttcacctctg tttctgtgaa aactgtgatg aaaatctgtg attcaaatag gttattttga
    13261 aagtaaacat cggttatgcg ataatcgcgc taatgtgatg taaaaagata caggggtgta
    13321 atcgtggcgg caaaagacag gattcaggca attaagcaaa tggttgccaa cgataaaaag
    13381 gtgacagtct caaatttgag tgggattttt caggtaaccg aagaaaccat tcgccgcgat
    13441 cttgagaagc tggaagatga aggctttttg accagaacct atggtggtgc tgttttaaat
    13501 acagcggtgt tgacggagaa tatccatttt tataagcgcg cttcatcgtt ttatgaagag
    13561 aagctgctta ttgcacgcaa ggcactaccc tttatcgaca ataaaaccac catggcagcc
    13621 gattccagta gtaccgtaat ggaattgctc aaattgttac aggaccgtag tgacctgacg
    13681 ttgctaacca actcggcgga agcgattcat gttctagctc agtcagaaat taaagtcgtt
    13741 tcaacgggtg gggaactaaa caaaaataca ctttccctgc aaggaagaat tactaaagag
    13801 atcatcagcc gctaccatgt cgatatcatg gtaatgagtt gtaaaggtct tgatattaac
    13861 agcggcgcgc tggactctaa tgaagcggaa gctgaaatca aaaagacaat gatccgtcag
    13921 gcgacagaag ttgcgttatt ggttgatcac tctaagtttg atcgtaaagc ttttgtccag
    13981 ttagctgact ttagtcatat taattatata ataactgata aatcaccggg tgcagagtgg
    14041 atagcatttt gcaaagacaa taatatccag ctggtgtggt aatttaagca cacgttgtca
    14101 atattatttg ttcctgcgac tggaaataaa atgatgataa agatgcggca agtgaatttg
    14161 ccgcaattgc taccttttat agcaaatccg aggcacccaa tggaacaata tgatcaaatt
    14221 ggcgcaagac tggaccgctt gcctttggcc cggtttcatt atcgtatatt tggtataata
    14281 agctttagtc tgttattaac ggggtttttg agttactcag gtaatgtcgt cttagcaaag
    14341 ctggtaagca atggatggtc aaataatttc ctctatgccg cctttacctc ggcattaatg
    14401 tttggttatt tcatcggctc acttactggt gggtttattg gtgactactt tgggcggcgc
    14461 agggcgtttc gcataaatct tctcatcgtc ggtattgctg caacaggggc cgcttttgtc
    14521 cctgatatgt actggctcat tttctttcgc ttcctgatgg gaacaggaat gggggcgctg
    14581 attatggttg gctatgcctc atttacggag tttatccccg cgacggtgcg tggaaaatgg
    14641 tccgcgcggc tctcatttgt tggtaactgg tcgcccatgc tgtctgcggc gataggcgtg
    14701 gtggttatcg ctttttttag ttggcgaata atgtttcttc tgggtggtat tggcatactg
    14761 ttagcctggt ttctctcagg taaatacttt atcgagtcgc cacgatggct ggaagggaaa
    14821 gggcaaatcg ccggtgcaga aagccaactt cgtgaagtag agcagcaaat tgaaagggag
    14881 aagagtattc gtttacccca gcttactttg aaccagagca acagcaaggt taaggtaatc
    14941 aagggtacct tcaggctcct gtttaaaggg gaaatgttac gacgtacatt agttgcgata
    15001 actgttttaa ttgcaatgaa catttcgctt tataccatta ccgtatggat accgaccata
    15061 tttgttaact ccggcattga tgtcgataaa tcaatattaa tgaccgctgt tattatgatt
    15121 ggtgctccgg taggaatatt tattgcggca ttaattattg atcattttcc tcgtcgatta
    15181 tttggctccg ccttacttat tattattgcc gtgttaggct atatctattc aagtcagact
    15241 acagagtggg cgattttaat ctatggtctg gtgatgatct tctttttata catgtatgtt
    15301 tgcttcgcgt cggcggttta tatcccggag ctttggccaa cgcatttacg cctgcgcggt
    15361 tcgggtttcg ttaatgccgt cggacggatc gtcgcagttt tcacgcccta tggcgttgcg
    15421 gcattattaa cacattatgg gtcgatcacg gtgtttatgg tgcttggtgt catgttagtg
    15481 ctctgtgcgc tggttctctc catttttggc atcgaaacgc ggaaggtgtc gctggaagag
    15541 atttctgagg tgaattaaat ctttcgttga tggcatcaat tgccagatgt accactgacg
    15601 tgttttattt ggcctacaat gccagtacag aattgtaggc cagcgtaaaa ttacccctgc
    15661 gtctcttccc agtctgccag cgtatatagc gttgccccag ctgctgacat ctccataaac
    15721 gcgtgtgcac tgtcctgggg ctggatattc acgccacgac agccatcggt aatcacgttt
    15781 accttataac ctaactgtaa cgcgtccagc acggtaaact tcacgcaata gtcagtagcc
    15841 aggcccataa cgatcaattc atcgatttca tgatcgcgca accagtcatc aagcgcggtt
    15901 ttctgccgac gtccgttatc aaaaaaggca ctgtaactgt caactaaagg attttcgcct
    15961 ttatggaaca ccgctgcgat ctctttttga ttcagtaacg gatgtaattg tgcgccttca
    16021 ctgttctgca cacagtgttc aggccagaaa gtttgccgca ggccgccgag ttggcctggc
    16081 gtataaggct ctacgccgtg ctggctggca aagctgccgt gattcgccgg atgccagtcc
    16141 tggctggcga taaccgcttc tccgcgcgac tggcaccagt caataaggcg gttagcaacc
    16201 tccaccgtac tgtcaccttc cggtacggcg agcgcgccac cagcacagaa atcattttgt
    16261 aaatcgacca gtaacagggc gcgatggggc attgcagtct ccttaatcat ccggcgtcag
    16321 ttcgccgcgc aggttttggc tcatggcctt gcgaatggtt tcagtatcca gttcctggct
    16381 cagcaggtaa tgcagtttgg ttagcgtggc ttctacagtc atatctgcac cgtcaataac
    16441 gccggcatgg gcgagggcgt taccggtggc ataaccactc atattcactt taccggacat
    16501 acattgtgtc aggttgacca ccacaatacc gcgatcgctg gcttcttgta attcctgcag
    16561 gaaggctttg ttttgtggcg cattacccac gccataggag cgcagaatca atgctttcac
    16621 cggttggcgc agaaaattgc gcacgacgtc agcagaaatc cctggataaa tcgtcaccac
    16681 gccaattggt tgtggcgtga ttggatgaac gattaattcc ccttcaccgt gcggggcggg
    16741 tggcgtattc aaacgacgaa tatggatacc agcttccagt aacggaggaa ggtttggaga
    16801 ggcaaacgca tcaaaaccat cggcatgggc tttggtagtg cggttgccgc gatacaatcg
    16861 gttattgaaa aagagcgtta cttcgttgat cggataattc ggcgcaacgt acaacgcatt
    16921 cagcagatta atttgtccgt cagagcgtaa ctcagccagc gggatttgtg accctgtcac
    16981 aatgaccggt ttaccgagat tctcgagcat gaacgacagc gcagaggcgg tatacgccat
    17041 cgtgtcggta ccgtgtagaa tgacaaaacc atcatagtcg tcatagtgcg ctttaatatc
    17101 ttcagcaata tgctgccagt cttccggcgt catatcagaa gaatccatca acggcgtata
    17161 ttcatgaatg gtgaaatctg gcatctccgg gcgatggaat tccggcatca gcgccagttg
    17221 gcgttgtaga tgacctgaca ccggaatata accctgctcg gaacgctgca tcccgatggt
    17281 cccgcccgtg taggcgacgt aaattgattt cttttgcatg atattgatat gaatgatatc
    17341 gaaagagcaa aagtatatac gtgaggcagg aggagcgcac ctgatactat gcggataagg
    17401 aaatatgggg cgagacagcg ggaagaggat tcgtcgaggt aaaaaataat gtggccgacg
    17461 atctgtcggc cactcagtac aagacttaac gcacgttggc gcaggtcagg caaaacgcat
    17521 aacggttttg tgggtcgtta aacgcggcca gcttgtcgct ttcactttta acggtagagg
    17581 ctaccgaggc aagcggtgca ggtaacatgg cctggaacgc atctggcaac attgcccgga
    17641 cagaaccaga catgttgtcc atcactttgt cgaagaaggt cggttcatca acgtagtatt
    17701 ccagatgcca ctgtttcact tttgcgagct ctgctgcttt ggcaaccgca tcatcgaaat
    17761 ccccgagact atcgaccagc ccgttagctt ttgcatcctg accggtccag acgtggccct
    17821 gggcgatttt atcaatttgc tccggcgtcg aatgacgcgc atcagcaacc agcgtgataa
    17881 agcgtttata gccattctca atgcttaatt gcatcatctg ctgcgcttcc ggcggcagtg
    17941 ccctggtgat agaaacatcc gccagcggtg aagttgagac accatcagta tgaacaccaa
    18001 tcgaatccag actattttct acggtggtga tcacgccgaa gataccgata gaaccggtca
    18061 gggtgctggg gttagccaca atgtaattag ctggcgtgga aatccagtaa ccaccagatg
    18121 ccgccatgcc gcccatcgat acaaccacag acttacccgc tgcccgggct gctgccagtt
    18181 cagcgcgaat cacttcagac gcggtaacgc tgccgcctgg gctattaaca cgcaggacaa
    18241 tcgctttcac tttcgggtca aggcgagcgt cgcggatttg tgccgcagtg gtatcaccgc
    18301 caacattccc ctgagtttcc tcgccatcca taattgcgcc attagcgaag acgacaccga
    18361 tgctgtcacc ggtatctgcc ggcgttttca atgcgtaatc gtaataactg atggcgcgat
    18421 aatttttatc agtcttactc cagccgaact ctttggtcag agctttttcg atttcggcac
    18481 tcgatgccag tgcatcgacc agcttgtttt ccagtgcata tttcgcggta tcgccaccgg
    18541 ttttggttaa accctcaagc aacccttgcg cgccagggaa tacctgctga gcagggatct
    18601 gccggttagc ggcaacagta ttcagatagt tttgccacag ctcaccaatc cagcggctgt
    18661 cagcttcgcg ggctgccggt gacatatcat cacgaataaa cggttcaacg gcagatttat
    18721 acgtacccac gcggaacaca tgggtggaaa ctttcagctt atccagcaac gatttgtagt
    18781 acagaccgtt ggtagcaaaa ccgtgcagat caaccacgcc ttgcggtgac agccaaattt
    18841 tattggcgaa actggcgaga taatattgcc cctggctgta gttctcgcca acggcataaa
    18901 ccggtttccc gctgtcacga aactctttca gggctttgcc gatgtactgc atagacggct
    18961 ggtcgccgcc tgcgaagttt ttcagatcca tcacgatacc ggtgatattg cggtcgtcct
    19021 tcgcctggcg aatggtgttg acgatatcaa acagtgagtt ttcctgcaga cgatcggaac
    19081 tggcaccaag cagctggcgg cttaatttac taaaccgctg agaactgtct ggtttatcga
    19141 cgatcacacc agaaatgtcc agcagcagtg cgccacgact ggccgtttct ttcgaatcac
    19201 caccactgac ctgcatccag atccccacac caaccagtac gaggaaaata aagaacaggt
    19261 taagtaccat ttcacggacg aaattcagca gacgccacgt ccatttaaaa aatccggcaa
    19321 taaatcgcca aagggttcgc atgtattctc ccaacttaag gtcacacctg tcacaggcgc
    19381 aatagatgtg gttatcgtaa tgacccaacc ggcacttgtc agcaggaatt gcggactgtg
    19441 ctgtaacaaa atccactctc gtgttaattt tgtgaataaa tatcacgaca ggagttaatc
    19501 aaatggatgc actcgaacta ttgatcaatc gccgtagcgc ctcccgcctg gctgaacccg
    19561 cgccaacggg tgaacaactg caaaacatcc tgcgtgcggg tatgcgtgcg ccggaccata
    19621 agtccatgca accgtggcat ttttttgtga ttgaagggga agggtgcgag cgtttcagcg
    19681 ccgtactgga acagggggcg attgctgccg gtagtgatga taaagctatc gacaaagccc
    19741 gtaatgcgcc gttccgcgca ccgctcatca tcacggtggt ggcgaaatgc aaagaaaatc
    19801 ataaagtccc gcgatgggaa caggaaatgt ctgccggatg cgcggtcatg gcgatgcaaa
    19861 tggcagccgt tgcccaggga tttggcggca tctggcgcag tggcgcatta actgaaagtc
    19921 cggtagtgcg tgaagcattc ggttgccgtg agcaggataa aattgtcggt tttctctacc
    19981 tcggcacgcc gcagttgaaa gcatctacgt cgattaacgt cccggacccg acgccgtttg
    20041 taacttattt ctgataattg tcgcgaaact gtctggattg tgagcaaagg cgcaggaatt
    20101 cagacactct cacttatcac ttcacggaat gagggttacc atacccggat tgcattgaca
    20161 ggagatgtcc atgagcgaga actcgattcg tttgacccaa tacagccacg gagctggttg
    20221 cggctgtaaa atttccccaa aagtgttgga aaccatcctg cacagtgagc aggcgaagtt
    20281 tgttgatccg aatttgcttg tgggtaatga aacccgcgac gatgcggcgg tgtacgatct
    20341 gggcaatggc accagcgtta tcagtaccac cgacttcttt atgccgatcg ttgataatcc
    20401 tttcgatttt ggccgcattg cggcgactaa cgccatcagc gatatcttcg cgatgggggg
    20461 caaaccgatt atggcgattg cgatcctcgg ctggccgatt aacaaacttt ccccggaaat
    20521 tgcccgcgaa gtaaccgaag gtggacgcta tgcatgccgt caggcgggta ttgcgctggc
    20581 tggcggtcac tccatcgatg cgccggagcc gatttttggt ctggcggtaa cggggatcgt
    20641 accgaccgag cgggtgaaga aaaacagtac cgcacaagcc ggatgcaaac tgttcctgac
    20701 gaaaccgctg gggatcggcg ttcttaccac ggctgagaaa aaatcactgt tgaaaccaga
    20761 acatcaggga ctggcgacgg aagtgatgtg ccggatgaac atcgcaggcg cgtcctttgc
    20821 caacatcgaa ggcgtaaaag cgatgaccga cgttacgggc tttggtctgc tgggccactt
    20881 gagcgaaatg tgtcaggggg ctggtgtgca ggcacgcgtc gactatgaag cgatcccgaa
    20941 actccccggt gttgaagagt acattaagtt gggcgcagta cctggcggca ctgaacgtaa
    21001 ctttgccagc tacggtcatc tgatgggtga aatgccgcgt gaagtgcgcg atctgctgtg
    21061 cgatccgcaa acttctggcg gtttgctgct ggcggtcatg ccggaagcag aaaatgaggt
    21121 taaaactaca gccgccgagt ttggcattga actgacggcg attggcgaac tggtgccagc
    21181 gcgcggcggt cgtgccatgg ttgagatccg ttaatttaat gcggttgttt attgccgaaa
    21241 aaccgagtct ggcgcgcgcc attgctgatg tcctgcccaa accgcaccgg aaaggcgatg
    21301 gctttatcga gtgcggtaat ggtcaggtgg tgacttggtg tatcggtcac ctgcttgagc
    21361 aggcgcagcc agacgcctac gacagccgct atgcgcgctg gaatcttgcg gatttgccga
    21421 ttgtcccgga aaagtggcaa ttacagccac gaccctccgt gaccaaacaa cttaacgtca
    21481 tcaaacgctt cctgcatgaa gccagcgaaa tcgttcacgc cggggacccg gatcgtgaag
    21541 ggcaattgct ggtggatgaa gtgctggact atctgcaact ggcaccggaa aagcgccagc
    21601 aggtgcagcg ttgcttgata aacgacctga acccgcaggc ggttgagcgg gcgatcgacc
    21661 gtcttcgttc caacagtgag tttgtgccat tgtgcgtttc tgcgctggcg cgagcgcgtg
    21721 ccgactggct gtacggcatc aatatgaccc gtgcgtatac cattctcggt cgcaatgccg
    21781 gttatcaggg cgtactttcc gtgggacgcg tgcagacacc cgtgctcggg ctggtggtgc
    21841 gccgcgatga agagattgaa aacttcgtgg cgaaagactt ctttgaagtc aaagcacata
    21901 tcgtgacacc tgccgatgag cggtttaccg ctatctggca accgagcgaa gcgtgtgaac
    21961 cgtaccagga tgaagaaggg cgcttgttac atcgtccact ggcggagcat gtggttaacc
    22021 gcattagtgg tcaaccggct attgtcacca gctataacga taaacgggaa tcagaatccg
    22081 cgccgctgcc gttttcgctt tcggcgttgc agattgaagc ggcaaaacgc ttcggtctga
    22141 gcgcgcagaa cgtgcttgat atctgccaga agctgtacga aacgcacaag ctaatcactt
    22201 atccgcgttc cgattgtcgc tatttgccag aagaacattt tgccggacgc cacgcggtga
    22261 tgaatgccat cagcgttcat gcaccggatc tgttgccgca gccagtggta gatccagata
    22321 tacgcaaccg ctgttgggat gacaaaaagg tcgatgcgca ccacgccatc atcccgaccg
    22381 cacggagttc tgcgatcaac ctgacggaga acgaagcgaa ggtctataac ctgattgccc
    22441 gtcaatacct gatgcagttc tgcccggatg cggtgttccg caagtgtgtt atcgaactgg
    22501 acattgccaa aggcaaattt gtcgctaaag cccgttttct tgctgaagca ggctggcgca
    22561 cgctgttagg cagcaaagag cgcgatgaag aaaacgacgg cacgccattg cctgtggtgg
    22621 cgaaaggcga tgagttgctg tgtgaaaaag gtgaagtggt agagcggcaa acccagccgc
    22681 cgcgccattt taccgatgca acactgcttt cggcgatgac cgggatcgcg cgctttgtgc
    22741 aggacaaaga tctgaaaaag gtccttcgtg cgaccgatgg tctggggaca gaagcaaccc
    22801 gtgccgggat tattgaactg ctgttcaagc gtggttttct gaccaaaaaa gggcgctata
    22861 tccactccac cgacgccgga aaagcgttat tccattcgct gccagaaatg gcgacgcgac
    22921 cggacatgac cgcgcactgg gaatcggtgc tgacgcaaat cagcgaaaag cagtgtcgct
    22981 atcaggactt tatgcagccg ctggtgggga cgctatatca gcttattgat caagccaaac
    23041 gtacgccggt gcggcagttt cgcggcattg tggctccggg cagtggtggc agtgctgata
    23101 agaaaaaggc tgcaccgcgt aaacgtagtg cgaaaaaaag tccgccagca gatgaagccg
    23161 gaagcggggc gatagcgtaa gcgagcgaat ctttcgtgct attcgagtca tattctgaaa
    23221 tatccagcgg atcaagaaag ttcgttggat atttttttgc atggataaaa ttatcgcctc
    23281 taaagtatgt aataacaggg aatgtgatga aaaaagtttt attgcaaaac catcctggga
    23341 gcgagaagta ttcttttaat ggctggggaa tatttaatag taattttgaa cgggtgatta
    23401 aagaaaataa ggccatgctg ctttgtaagt gggggtttta tttaacatgt gttgtcgctg
    23461 taatgtttgt attcgcagcg ataacatcca acggtttgaa tgaaagaggc ctgattaccg
    23521 cgggatgctc ttttctttat ctattaatta tgatggggct tattgttcgg gccggtttta
    23581 aagcaaaaaa agaacaactg cattattatc aggctaaagg tattgagccg ctcagtatcg
    23641 agaagttaca ggcgctacaa ttgatcgcac cttatcgatt ctatcataag caatggtctg
    23701 aaacgctaga gttctggccg cgaaagcctg aacctggcaa agataccttc caatatcatg
    23761 tgcttccttt tgactcgatc gatatcatca gtaaaagacg cgagtcttta gagaatcaat
    23821 ggggtatcga agatagcgaa agttattgtg ccttaatgga gcattttctt tctggcgacc
    23881 atggagccaa tacctttaaa gcaaacatgg aggaagcccc agagcaggtt atcgccttgt
    23941 tgaataaatt tgctgttttt ccctcagact atatctctga ttgcgctaat aatcgctccg
    24001 gtaaatcctc ggcgaagcta atatgggcgg cggaattatc atggatgatc tcgatatcaa
    24061 gcacagcttt tcaaaacggg acaattgaag aagaactggc ctggcattat ataatgcttg
    24121 cttctcgaaa ggcgcacgag ttgttcgaaa gcgaagaaga ttatcaaaaa aatagtctaa
    24181 tgggatttct ttactggcat atctgctgct atcgcagaaa gttaacggat gctgaacttg
    24241 aagcgtgtta tcgttacgac aagcagtttt gggagcacta cagtaagaaa tgccgttggc
    24301 ccataagaaa tgttccctgg ggagcatcat ccgttaaata ctcataaacg cctgaaattt
    24361 tgccgggggc gctgcgcttg accggcctac attagagcgt aactgcaatg aattgtgccc
    24421 atctgtaggc ctgataagcg tagcgccatc aggcatttac aacttaaatc acaccctgcg
    24481 ccagcatcgc atcggcaacc ttcacaaaac cggcaatgtt cgcgccctgc acgtagttgg
    24541 tttgctcacc ttcaccacca tgctcaacac aggcatggtg gatatccagc atgatgtgat
    24601 gcaaacgtgc gtcaactttc tcggctttcc agcccaggcg cgcagcgttt tgtgccattt
    24661 ccaggcccga cgttgctaca ccgccagcat tagccgcttt acccggtgca aacagtacgc
    24721 ctgcctgctg gaacagttca gtcgcttcga tggtggtcgg catatttgcc ccttcagcga
    24781 cggctttaac gccattggcg ataagctgat gcgcggcgtc aacatccagt tcgttctggg
    24841 tggcgcaagg cagggcgata tcaaccggta cagaccacgg ctgttggcct tcgagataga
    24901 ccagaccaaa ttctttggcg taatctgccg ctcgaccatc gcggctggct ttgatttcga
    24961 taagacgtgc cagtttctct ttcgtgaatc cgctttcatc aactacagtg ccgctggagt
    25021 ctgacgcagt gatcacacga gcaccaaatt ccatcgcttt ttcgatagcg tactgggcga
    25081 cgttgccgga gccagaaacg gaaacgctca tcccttcaaa acccataccg tggcgtttta
    25141 acattgcttc tgtgaaataa accagaccgt agccggtagc ttccgggcga ataagactac
    25201 cgccaaatga aaggccctta ccggtgaaga cgcaggcggt attgttggag agctttttca
    25261 tcatccccgc cataaagccg acttcacgac caccaacgcc gatatcacct gccggaacgt
    25321 cggtatccgc gcccaggtgg cgatacagtt cagtcatcag cgtctggcaa aaacgcatca
    25381 ctttaccttc gctttttcct ttcggatcga aatcgctgcc gcctttacca ccgcccatcg
    25441 gcagggtagt cagggcattt ttgaaggttt gttcaaagcc gaggaatttg agaatggaaa
    25501 ggttaactga cggatggaag cgcataccgc ctttgtacgg gccgatggca gagctgaact
    25561 gcacacgcca tgcacggttg acctgtatct ggttgcgatc atcaacccat accacgcgaa
    25621 actggatcac gcgctccggt tcaaccagac gctccagta
//
LOCUS       DAEBXN010000056        25627 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.056, whole genome shotgun sequence.
ACCESSION   DAEBXN010000056 DAEBXN010000000
VERSION     DAEBXN010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25627
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.056"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            149..406
                     /locus_tag="KJL99_002349"
     CDS             149..406
                     /locus_tag="KJL99_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4437053.1"
                     /translation="MDQEMTFSLSYEQLTRFAEKRIRECNLDSQGAIYLCESAKAGAV
                     LIFWHELAINGYASMNAIKRQELIDADFQRLRNLIWPEDDR"
     gene            406..996
                     /locus_tag="KJL99_002350"
     CDS             406..996
                     /locus_tag="KJL99_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4437054.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDLERVLERSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRIRAEQR
                     AAEVVVLPLVPAAGLPADGTSATDLAVELNGITYQACRGDFVVRLDGSTCLQLWNKEG
                     RVVRREGDPLEVAQWLQACHDAGMEVRVQVNESVTP"
     gene            complement(1014..1361)
                     /locus_tag="KJL99_002351"
     CDS             complement(1014..1361)
                     /locus_tag="KJL99_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4437055.1"
                     /translation="MTFEQKKARAIALMDSKKMWRSNYAPPLLRILWRLGIRLPPLPF
                     MPFWQVTVLTGGLWGISWGCAMWFIYWGPSGMVAGEAIIISITGGFWFGLLMASFHWW
                     RRKVNRLPPWDNV"
     gene            1490..1825
                     /locus_tag="KJL99_002352"
     CDS             1490..1825
                     /locus_tag="KJL99_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin transporter"
                     /protein_id="HBD4437056.1"
                     /translation="MNRKYYFNNMWWGWVTGGYMLYMSWDYDFKYRLLFWCISLCGMV
                     LYPVAKWCIEDTVLKFTRPDFWNSGFFADTPGKMGLLAVYTGTVFILSLPLSMIYILS
                     VIIKRLSVR"
     gene            complement(1847..3727)
                     /locus_tag="KJL99_002353"
     CDS             complement(1847..3727)
                     /locus_tag="KJL99_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001610315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin 1A"
                     /protein_id="HBD4437057.1"
                     /translation="MSDPVRITNPGAESLGYDSDGHEIMAVDIYVNPPRVDVFHGTPP
                     AWSSFGNKTIWGGNEWVDDSPTRSDIEKRDKEIAAYKNTLSAQQKENENKRTEAGKRL
                     SAAIAAREKDENTLKTLRAGNADAADITRQEFRLLQAELREYGFRTEIAGYDALRLHT
                     ESRMLFADADSLRISPREARSLIEQAEKRQKDAQNADKKAADMLAEYERRKGILDTRL
                     SELEKNGGAALAVLDAQQARLLGQQTRNDRAISEARNKLSSVTESLNTARNALTRAEQ
                     QLTQQKNTPDGKTIVSPEKFPGRSSTNHSIVVSGDPRFAGTIKITTSAVIDNRANLNY
                     LLTHSGLDYKRNILNDRNPVVTEDVEGDKKIYNAEVAEWDKLRQRLLDARNKITSAES
                     AVNSARNNLSARTNEQKHANDALNALLKEKENIRNQLAGINQKIAEEKRKQDELKATK
                     DAINFTTEFLKSVSEKYGAKAEQLAREMAGQAKGKKIRNVEEALKTYEKYRADINKKI
                     NAKDRAAIAAALESVKLSDISSNLNRFSRGLGYAGKFTSLADWITEFGKAVRTENWRP
                     LFVKTEAIIAGNAATALVALVFSILTGSALGIIGYGLLMAVTGALIDESLVEKANKFW
                     GI"
     gene            complement(4006..5352)
                     /locus_tag="KJL99_002354"
     CDS             complement(4006..5352)
                     /locus_tag="KJL99_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001632353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4437058.1"
                     /translation="MPMILVKKNPRGKVIRELSSEEETAVKTVCGLKRPATMAQHNLA
                     NDLLREMREYDAWLQCDCIPGDSPAMNFAALKNNTGTLYLSSFNHEHAPECPMYRQLS
                     GNEEETSFGASRHPVSTRINYRNFLPPDDSNSVIRLQARSAYHNGERSSVRKKRPRLG
                     RLLLSLIEDAGLNKLDSLANPRIRTNYRECLDAIRQVTLQQEYIRGRALSEIIHFRPG
                     MSERSQERLMETLENSERHWPARRKHMFFQIFMAQHICRDAVEIHWANGNIQVIRPVR
                     GISINGEAQGGIRPPYWVILAFCRSADGRIICSEGYAHALYQLTCPVPVDSKLERNTL
                     TALLNVASWLKRKPGTPELSLERPLFDTEVYVNGEKKYVLPDFIVTARAPDGKTARVV
                     IETMGYEDSDYCARKSRQHTGMKQIGVLHTDPPKWLDNDHPPFKKHMYGVFMHLRY"
     gene            5706..6314
                     /locus_tag="KJL99_002355"
     CDS             5706..6314
                     /locus_tag="KJL99_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proQ/FINO family protein"
                     /protein_id="HBD4437059.1"
                     /translation="MGKNQEERKTPIIVVKKRRTFSPPSLSEKTDIIAPVFTEQTAES
                     APAGINSSAVETHIPEAPARKKKKKKKRHRFPRPSHWTREYTHECVEKIKILFPHLRA
                     EGGGFIPLKIGINNDISAFLAEHPETELTMDEWLCAVSCITSRRVYLQRTAVAGVPRY
                     GLDGHPKGQVSESEAQSAGRRLVTLEQKLLRMQAQQENISGQ"
     gene            6370..6879
                     /locus_tag="KJL99_002356"
     CDS             6370..6879
                     /locus_tag="KJL99_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4437060.1"
                     /translation="MSKSGAEKIQFILNNHEQAYRRLKPVYTFLELASWISVVLLIIY
                     LSGKNHVLTAAFLCFAWTAFWVLFLLHYFPKVFFILDRQSLLNLMEATKDSPDVKQEL
                     LNRLFSGKRMTGRDEMDICRLLRKNEEEKIRQSELNIIRNFIDEQQLTNSDIPKKSTV
                     PNEDKKGIR"
     gene            complement(7094..7252)
                     /locus_tag="KJL99_002357"
     CDS             complement(7094..7252)
                     /locus_tag="KJL99_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001700046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4437061.1"
                     /translation="MAESGYGLAGLGMGKVKSINQYRLTPGFGGFTPVSHVTTACRLP
                     CRWRGISW"
     gene            complement(7950..8981)
                     /locus_tag="KJL99_002358"
     CDS             complement(7950..8981)
                     /locus_tag="KJL99_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBD4437062.1"
                     /translation="MAGLKNTPYNAVHWSQLAPEEQIRFWEDYEAGRATTFLVEPERK
                     LTNRRRGEHSTKPKCENPSWYRPERYKALKGQLGHAYNRLVKKDPVTGEQSLRMRMSR
                     HPFYVQKRTFVGRKYAFRPEKQRLLDAIWPVLVSFSDAGTHTVGMSVTRLAEEISPKD
                     SEGHVIPELEVTVSRLSRLLAEQVRFGVLGVSEETMWDREHRQRLPRYVWITPAGWQM
                     LGVDMVKLHEQQQKRLRESEIRQQLIREGVLREDEDISVHAARKRWYLQRSQDALKKR
                     REKAAASKRANRLKKLPVDQQIYEMAEYLRKRLPPDEAYFCSDDHLKRLAIRELRQLE
                     LTLAAPPPH"
     gene            9954..10217
                     /locus_tag="KJL99_002359"
     CDS             9954..10217
                     /locus_tag="KJL99_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HBD4437063.1"
                     /translation="MPYHKDRNLYFLSSSMVAQAGQALAWPVSDDAGIPTPVWAITNE
                     RRNSVIAFLVVIGDRCYVTSNDTETPGHHSVGRHVSRAINKPA"
     gene            10135..10422
                     /locus_tag="KJL99_002360"
     CDS             10135..10422
                     /locus_tag="KJL99_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraA"
                     /protein_id="HBD4437064.1"
                     /translation="MSPQMIQKLPAITLLEGMFPELSTNQLKVCVFYAMGVPYDAIAQ
                     NCRLSPETVRTYLKRSLKNLNLEGYDALRSAVLMRTFVFMISNTAKENEKM"
     gene            10864..11397
                     /locus_tag="KJL99_002361"
     CDS             10864..11397
                     /locus_tag="KJL99_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001303319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusG"
                     /protein_id="HBD4437065.1"
                     /translation="MNIEHLNNRNWYLAQYNTAGKNRESLFSWLNEQNVVPWTPLITR
                     KIRRADSRCCYRERIFAIFPGYFFILANFDIHPVSALRRHSAFIDFVKFGGEIKPVNK
                     DIVDGLMKIYPDPVLNPGAREELNAASSIWLTKAQYQYLLRMENTLQPESRISLLLEL
                     VSNAEHHGFMERLVNIP"
     gene            11651..12334
                     /locus_tag="KJL99_002362"
     CDS             11651..12334
                     /locus_tag="KJL99_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraC"
                     /protein_id="HBD4437066.1"
                     /translation="MTTTEHPVRQLLWCALNALKTAQENHNITSETGLRHYLLEWLSG
                     ASRHPEFRSLPGEIMALKALTEKDRNIPIIGTLNTLFLSSATVGECPLFRFRAALGKL
                     QKAGWRTYVCPWPERVFNESIERACSGKRHLLQLSRTEECFLPTGDMRAPVTLQLITP
                     LHRKTMQLLETAELIFSDEGFQVVRGYEHQFGVERRETLFHTLFIGLPSLPEDVWGAT
                     QEQTTITLH"
     gene            12507..13058
                     /locus_tag="KJL99_002363"
     CDS             12507..13058
                     /locus_tag="KJL99_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4437067.1"
                     /translation="MKKQPLLWMTIAAALLSGCTGHREHHPQVTFTVDTVRTDTTASN
                     PICLAEKYGSLLNERHPDLFAGAQGSISRSAFFNDSLTLTPETLHGMIQNTPCVESLV
                     TSVGPDTFTADVSPGVRMQVSPDIHGAPGNITLRLSTTDQKRKQTVEQSISLRPEQSL
                     VVNGFTGDGAGEKRVVLITPHVR"
     gene            13220..13474
                     /locus_tag="KJL99_002364"
     CDS             13220..13474
                     /locus_tag="KJL99_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilI type IV pilus biogenesis protein"
                     /protein_id="HBD4437068.1"
                     /translation="MPQQHPGRLQILVVDAHCKRRLFSTKTPTDPDELARRFCTPDNC
                     LVVVLRDNRFLFRLERAPGSHCRWHKGSSSRHQHLQDWLS"
     gene            13641..13937
                     /locus_tag="KJL99_002365"
     CDS             13641..13937
                     /locus_tag="KJL99_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilJ"
                     /protein_id="HBD4437069.1"
                     /translation="MFTMFFLVKLTIAIVIVCGILSPILVSPHNKLSTLFWKIPNAFW
                     IFLICIYFAIAMAVLIESSEQTRIRRQQYFQQYEVPNETHKNVVIENSIKSQLI"
     gene            14561..15628
                     /gene="pilL"
                     /locus_tag="KJL99_002366"
     CDS             14561..15628
                     /gene="pilL"
                     /locus_tag="KJL99_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001360287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis lipoprotein PilL"
                     /protein_id="HBD4437070.1"
                     /translation="MKKNCLTLILPSLLSGCAGISGSNNVAPSDTPALVFVDGQISES
                     AGVLALTQQRLSPPKITPPPRVSVPETVKTAQPVSESRPLPSPTISQNFSRLIITGKP
                     GSAPVTLSPTARNRTVSQWIKTLLPEGWKFRQENAATPKLNTKLVSWSANDQWTRSLN
                     RLLEEQHLWGHLDWDNKTLTVTTSAAAPAQDTATTISPAAMVTTSTSPESPTTANSQN
                     KPRNPFRGNSVSSSTPAATPTPTGSTVKSIPLMTGTPVKPVSQGKEWRAPAGTTLREN
                     IIKWAEETKCESMASTHWMVIWPLSVTDYRLDAPLMFRGSFESAMEQVFELYRTAQKP
                     LYAQASRMQCIITVSDTRDGR"
     gene            15628..16065
                     /gene="pilM"
                     /locus_tag="KJL99_002367"
     CDS             15628..16065
                     /gene="pilM"
                     /locus_tag="KJL99_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HBD4437071.1"
                     /translation="MGWLVMAVGLTILIITGSYQNQKMSETTNAQQYASASVWASQIL
                     MIANRINDIRYVSGQQDGVISSDKLALPVTPDSRIKHQLQQGRLWVWMPEQPGLVETL
                     RSKSRGSALIGIFQNGQLTWLSGTATGLTPPAGITAGSVVYVN"
     gene            16079..17761
                     /locus_tag="KJL99_002368"
     CDS             16079..17761
                     /locus_tag="KJL99_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN family type IVB pilus formation outer
                     membrane protein"
                     /protein_id="HBD4437072.1"
                     /translation="MKKSHQRSMKLAVLPCMIAVALSISGCTFSEINKMQKKAQEDSA
                     HAREKVSALSARKSQALTWLDNQWINPVPVAQVSREKKQTAPACYITQARKGEITLQE
                     LGQRITAVCGIPVIITPDAANSTLEGGATRQMTGTLPAPDENGRLPLSSLGSTTMTTS
                     TQPLTLNNLMWQGDINGLLDLMASRSGLYWRMDNGRIVFYLTETRTYPLHMLNTKTSS
                     SSSVSSGSTSTMGATGGQDNSASGDATSSQSTTVGQEYDLYEDIRKTIEAMLTPEKGR
                     YWLSASSSTLTVTDTPAVQEAVARYVDEQNSIMNRQVALNVQVLSVSNTRNEQFGLDW
                     NLVYKSLHSAGATLNNASGDFTGATSAGVSILDTATGNAAKFSGSSLLIKALSEQGDV
                     SVVTSQESTVTNLTPVPIQMADQTVYVAQSATTTTTDVGATTTLTPGMITTGFNMTLL
                     PLIQKTGNLQLQMNFNLSDPPTIRSFTSKDGNSYIEMPYTKLRSLSQKVNLKEGQSLV
                     VTGFDQNNTTTSKAGTFTPANPLFGGSQTGKNERSTLVIIITPTFPSGGNNG"
     gene            17754..19049
                     /gene="pilO2"
                     /locus_tag="KJL99_002369"
     CDS             17754..19049
                     /gene="pilO2"
                     /locus_tag="KJL99_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4b pilus protein PilO2"
                     /protein_id="HBD4437073.1"
                     /translation="MADEDINPVVLLEDPKVNHRVWAACLKWTPVVKKQRVPSHHKHK
                     SHVKPRRLTSLKVTVGSRNSRGKISRLTGTGILTRPERNHYFSLALAFCSWVRNGYGV
                     FRYSDKELLFLASINGQPAVMADLSGNDADVAQKVSLFLAMNEEPPEKWQVVSSLEHP
                     DNWESIITRLSSADLRRCKLTVGNRSKFTLPAVLFLVAAGAGTVFWMTQPEPDVVPTP
                     EEIAARARLQFKKPDPPPELPHPWASQPVISDFLKACADLRKPSPVALEGWKLTGGTC
                     TPETFTLIYERQPGGTIEGFLARSKEIFGVIPDFNLKDGARQVSVTRPLPSLPGRDEA
                     VPAPSEQLMRVFTWFQKKQLTPAINEIAIPEPLPGNDGEPAPVQKWKEYQFSLSTPVN
                     PDEFFPLLQDTGVRLSNIHFELNGGTFSYSSEGHIYASK"
     gene            19036..19488
                     /gene="pilP"
                     /locus_tag="KJL99_002370"
     CDS             19036..19488
                     /gene="pilP"
                     /locus_tag="KJL99_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilP"
                     /protein_id="HBD4437074.1"
                     /translation="MRPSKLLIIPSIFFFSGFSFATTQPLVTIGELEAQQNRNILLQA
                     KVQGAQLQKQLEESDVTSSSETVSGFSGVTAPLPSVSEQTAPKNRKELPIIMEINGKD
                     KRLHAVLRMADGRQTSVTTGSQLPGTSVTVKSISLSGVTLSDGTTLTF"
     gene            19499..21052
                     /gene="tadA"
                     /locus_tag="KJL99_002371"
     CDS             19499..21052
                     /gene="tadA"
                     /locus_tag="KJL99_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HBD4437075.1"
                     /translation="MDKYNLKDALLFISRGDTHEILIETNQRTRPDVQSNLQELLKLY
                     PGINPKVVSLSELQEAGQDEKNRGPKERIIHLKDLADVSESEKKVLSYFETARKLGAS
                     DIHFLISESIFKVRMRIFGELQTVDEDQPALGYSLCATAILSMADVTETSFFPQREQD
                     ARLSPQLMRKIGIFGARYSHRPTGDGLIAVMRLIPDDGDKVPTFKQLGFIPEQIRLLN
                     IMLRRPEGKIVLSGPTGSGKSTTLRSACRVYLDDNQGRHLLTIEDPLEGQILGATQTP
                     IICDKSDEDAVKLAWSRAISSAMRLDPDAIMEGEMRDLISMMSTTYAAQTGHIVLTTL
                     HTNSALGIPERMITMGMNADLICDAQLLIGMISQRLVPTLCPSCRIPWETRAPELRDD
                     ERDYLERHCNKDSLCSTDNIWFRNPHGCSECNHDVIINGRKRGEIGKGLTGRTVIAEV
                     IEPDNRLFQILKTRGKVAARKYWLENMKGISRVEHLLRRINEGLVDPLEADRIIPLDE
                     DERLSIDDV"
     gene            21065..22162
                     /locus_tag="KJL99_002372"
     CDS             21065..22162
                     /locus_tag="KJL99_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001699973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HBD4437076.1"
                     /translation="MREMNFSQRLRRFIVRKTFSAPYRVQFYEALRFLLENKQPLKTA
                     LEQMRDAWTDFGRKWHPFAELATDCIESLRENSGENSLEYTLSLWVPQEEAAVISAGI
                     RSGSIVDALQFATTLTDAKEQIHQAIWQMAIYPVGLLIMMTGTLYVLNTQLIPELSKI
                     SSPDSWSGALGFLYGLSVFVDNYGAICAVLFAVITGLISWSLPNWKSPDSVRTFADKI
                     MPWSIYQDIQGATFLLNMAALLKAKMTTLNSLNILQEFASPWLSTRLDSIIYRVRQGD
                     HLGLALRQCGYQFPSREAANFLSLLQGDGATELISNYGQRWLSQTLQRVKKRANVIRL
                     IMLIFLVMSLMLLVFAIMDIQSISDNSMGNF"
     gene            22180..22794
                     /locus_tag="KJL99_002373"
     CDS             22180..22794
                     /locus_tag="KJL99_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilX"
                     /protein_id="HBD4437077.1"
                     /translation="MLVENINATLTGNNKKNEPHDKGWGILEQGTIALVVLFVIVVVL
                     GSLYALRTRTNVATETANIQTIITSAQSLLKGSDGYTFTSSAKMTGALIQMGAVPSGM
                     TVQGDKTSGTATLYNAWGGAVTVAPASTSGFNNGFTVTYDKVPQDACIQIATRISKTG
                     LTNGITLNSTAHSDGKVTTEEASTQCKADNGSTGTNKLIFTING"
     gene            22804..23364
                     /locus_tag="KJL99_002374"
     CDS             22804..23364
                     /locus_tag="KJL99_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HBD4437078.1"
                     /translation="MSGGTVPLRLISLILLFSLIPPARASYVAPKQIWTNKWDNCFAA
                     AGARYQIEPLLLKAISAGESSLRPGAININKDKKTGKASSTDYGLMQINSTHIPKLIN
                     MGVIKKSEDLITKPCLNIHIGSWILARHFQICGVSWNCLGSYNAGFRKDRHETREQYA
                     NKIWRIYRDMKGICLPGQGGRQCRQS"
     gene            23349..24005
                     /locus_tag="KJL99_002375"
     CDS             23349..24005
                     /locus_tag="KJL99_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBD4437079.1"
                     /translation="MQTIVGISLSPVYILPLMFTFSIIGRTLLFRVRYFLSDTGHLWY
                     KTHPAVLSGIWLYSIAVALIILSSSPLLYRIHAVLILSFILQMAVTDALTGLLPGTFT
                     RRFLIAGMLSQITTDIWWFRTTEFATAAIVLFCLHKLVNRHRLNIGTGDLWLIAGITA
                     WSGLYNAIWCVLLGTGGFVLWHSTWCIKGHKEGPLGPWLCFGHVLLLLDNLYQPLWVI
                     "
     gene            24005..25429
                     /gene="pilV"
                     /locus_tag="KJL99_002376"
     CDS             24005..25429
                     /gene="pilV"
                     /locus_tag="KJL99_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="HBD4437080.1"
                     /translation="MKKYDRGWASLETGAALLIVMLLIAWGAGIWQDYIQTKGWQTEA
                     RLVSNWTSAARSYIGKNYTTLQGSSTTTTPAVITTTMLKNTGFLSSGFTETNSEGQRL
                     QAYVVRNAQNPELLQAMVVSSGGTPYPVKALIQMAKDITTGLGGYIQDGKTATGALRS
                     WSVALSNYGAKSGNGHIAVLLSTDELSGAAEDTDRLYRFQVNGRPDLNKMHTAIDMGS
                     NNLNNVGAVNAQTGNFSGNVNGVNGTFSGQVKGNSGNFDVNVTAGGDIRSNNGWLITR
                     NSKGWLNETHGGGFYMSDGSWVRSVNNKGIYTGGQVKGGTVRADGRLYTGEYLQLERT
                     AVAGASCSPNGLVGRDNTGAILSCQSGTWKTSGSLNGSYTNLGSHRGSFSGRNSGGST
                     LFIYASGGNGGSAGGACANTSRLQGYVGGTLISVNASNNPAYGKTAFISFAVPAGTSY
                     QITSYPTENTSCGAGVFSVFGYQT"
     gene            complement(25426..>25627)
                     /locus_tag="KJL99_002377"
     CDS             complement(25426..>25627)
                     /locus_tag="KJL99_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4437081.1"
                     /translation="TIGGKLKVTQLSTTGYLGQFDFCAIARMGNAEDAHYCQVVESPA
                     GSRKWYKYEHKTGCIASCVTLN"
BASE COUNT         6899 a         6186 c         5888 g         6654 t
ORIGIN      
        1 taataaaaaa cacacataaa atcatatcag atatgatttt catcattaat catgaaacta
       61 tatctgatat tattaataat caataacaca taaatcattc aggatatgat ttttacctgt
      121 cagaacatta tcagatggag cgtgcaggat ggatcaggaa atgacgtttt cactcagcta
      181 tgaacaactg acccggtttg cggaaaaacg catccgggaa tgcaatctgg acagccaggg
      241 cgcgatttat ctctgcgagt cagccaaagc cggagccgtg ctgatcttct ggcatgaact
      301 ggccataaat ggctatgcct caatgaatgc cataaagaga caggagctca ttgacgccga
      361 ctttcagcgc ctcagaaacc tcatctggcc ggaggatgac aggtaatgac ccagagccgc
      421 cgaccgtcgc cactgcagcg gagagtgctg attgtgctgg ctgcccttga tgaaaaacgg
      481 ccgggacccg tgctgacgcg ggacctggaa cgggtgctgg agcgaagcgg ggaagccccg
      541 gtctacgggc caaacctgcg cgcctcctgc cgtcgtctgg aggatgccgg ctggctgcgt
      601 accctgcgcg ctccgaacct gcagctggca gtggagctga cggacgccgg ccgggccgtg
      661 gcacagcctc tgctgctggc agaacaggac cgcattcggg cagaacagcg tgcggcagaa
      721 gtcgtcgtac tgccgctggt cccggcagca ggtctgcccg cagacggcac ttctgccact
      781 gatctggcgg tggagctgaa cggcataacg tatcaggcct gccgtgggga ttttgtggta
      841 cgtctcgacg gcagcacctg tctgcagtta tggaataaag aaggcagggt ggtacgccgg
      901 gagggcgatc cgctggaagt ggcgcagtgg ctgcaggcct gtcacgatgc cggaatggag
      961 gttcgtgtac aggttaatga aagcgtcacc ccgtaaaaac ataccagcac ggtttacacg
     1021 ttatcccatg gtggcagccg gttaactttc ctgcgccacc agtggaaaga ggccatgagc
     1081 agtccgaacc aaaaaccccc cgtgatactt ataataatgg cctcacctgc caccattcct
     1141 gacggccccc agtagataaa ccacattgca catccccagg aaatgcccca taagcccccc
     1201 gtcagcaccg tgacctgcca gaacggcata aagggtaatg gcggaagccg gatacccagc
     1261 cgccagagaa tacgcagaag aggaggggca taattgctcc gccacatctt tttgctgtcc
     1321 atcagggcaa tggcccgggc ttttttctgc tcaaaggtca caggagcacc tccgttccat
     1381 gatgtaaacg tgacacagct tattgttatt gattattatt atcatgctgt ggttatgttg
     1441 tcatgcaccg gtacactgac aaaagtgcaa aagaaaggaa aacaggcaga tgaacagaaa
     1501 atattatttt aataatatgt ggtggggatg ggtgacgggg ggatatatgc tgtatatgtc
     1561 atgggattat gattttaaat acagattact gttctggtgt atttctctct gcggaatggt
     1621 tttgtatccg gttgcaaaat ggtgtattga agatacagtt ctgaaattta cccggcctga
     1681 tttctggaac agcggttttt ttgctgatac acctggaaaa atgggattgc ttgctgttta
     1741 tacgggtact gttttcatat tatctcttcc gttaagtatg atatatattc tttctgttat
     1801 tataaaaagg ctgtctgtaa gatagacagc ctgtcttata ttttatttaa ataccccaga
     1861 acttattcgc tttttccaca agcgattcat caatcagcgc accggtgaca gccatcagta
     1921 aaccataccc gataatgcct aaagcgcttc cggtaagaat actgaagacc agtgcaacaa
     1981 gagccgttgc ggcattgcct gctataatgg cttctgtttt aacaaaaaga ggacgccagt
     2041 tctctgtccg gacagcctta ccaaactcag tgatccagtc agcaagactt gtaaattttc
     2101 ctgcatatcc cagtccccga ctgaatctgt tcagattaga cgatatatca gacagcttca
     2161 cagactcaag ggctgcggca atcgctgcac gatcttttgc attaattttt ttgttaatgt
     2221 cagcccggta cttttcatac gtttttaatg cctcttcaac attacgtatt ttcttccctt
     2281 tagcctgccc ggccatctct ctggctaact gctcagcttt tgcaccatat ttttctgaaa
     2341 ctgatttcag gaactctgtt gtgaaattaa ttgcgtcttt cgttgccttc agttcatcct
     2401 gttttctttt ctcttccgct atcttctgat tgatgccggc aagctggtta cggatattct
     2461 ctttttcctt caacagggca ttaagagcgt catttgcatg cttttgctca tttgttctgg
     2521 cactgaggtt atttctcgcc gaatttaccg cagattcagc agaggtgatt ttatttctgg
     2581 catcaagcaa tcgttgccgt aacttatccc attcagcaac ttcagcatta taaattttct
     2641 tgtcaccttc cacatcctct gtcaccaccg gattccggtc attcagaata ttgcgtttat
     2701 aatccagacc ggaatgggtc agaagataat tcaggtttgc acggttatcg atgactgcgc
     2761 tggttgtgat ttttatcgta ccggcaaatc tcggatcacc gctcacaaca atagaatgat
     2821 ttgttgatga acgccccggg aatttttcag gggaaactat cgttttgccg tcaggcgtgt
     2881 ttttctgttg cgtcagctgt tgttcagctc tggttaatgc attacgggcc gtgttaagcg
     2941 attccgtcac tgaactgagt ttattccggg cctctgaaat ggccctgtca ttccgtgtct
     3001 gctgcccgag cagacgggcc tgttgtgcat caagaacggc aagggctgcc ccgccatttt
     3061 tttccagctc tgacaaccgc gtgtccagaa taccttttct gcgctcgtat tcagcaagca
     3121 tatcagcggc cttcttgtct gcgttctgcg catccttctg ccgtttttca gcctgttcga
     3181 ttaacgacct ggcctcccgg ggagatatac gaagagaatc agcatcagca aacagcatcc
     3241 ggctctctgt atgcagccgg agggcgtcat atccggcgat ttcagtacgg aatccgtatt
     3301 ctctcagctc tgcctgcagg agtctgaact cctgtcgtgt aatatcagcg gcatctgcgt
     3361 ttccggcacg gagtgttttc agtgtgtttt catctttttc ccttgcagca atcgccgcag
     3421 agaggcgttt tccggcttca gtgcgcttat tctcattttc tttctgctgc gcgctgagcg
     3481 tgtttttgta cgctgcgatt tccttgtccc ttttttcgat atcacttcgg gttggggaat
     3541 catcgaccca ctcgtttccg ccccagatgg ttttgttccc gaaggaactc catgcaggcg
     3601 gggtaccatg aaagacatcg acacgtggag ggtttacata aatatcaacg gccataattt
     3661 catggccatc tgaatcatac cccagcgatt ctgcaccggg atttgtaata cgtacagggt
     3721 cagacattcg gtatctcctt catcctctga ggaaaacaaa tgaattaact gcttacgtat
     3781 atggatacat atacagtatt aatttattaa ggagaaaatg gcatgtcaag atttgatgat
     3841 agtgattatc attaacatga agtgatgtta caccgttcag aaagcctgct gacgctgtgg
     3901 cagcatcaca gtctgggtat tctctggtgc aatacaaaaa ccggactttc ccgtaacaac
     3961 agacaatgtt acgggaaaag ttacagaact gagccacaaa atatctcagt acctgagatg
     4021 cataaaaaca ccgtacatat gtttcttaaa agggggatga tcgttatcca gccatttcgg
     4081 tggatcggta tgcagaacac caatctgctt catgccggta tgctgcctgg attttctcgc
     4141 gcagtaatca ctgtcttcat atcccatcgt ttcgatgacc actctggccg tctttccgtc
     4201 aggagccctt gctgtgacaa tgaaatccgg cagtacatat ttcttttcac cattaacata
     4261 aacttctgta tcaaacaggg gcctttccag acttaattcc ggcgtacctg gctttctttt
     4321 aagccagctg gccacattca gaagagcagt gagcgtgttt cgttccagtt tgctgtccac
     4381 aggcaccgga catgtcagtt gataaagagc atgagcatac ccttcactgc agatgattct
     4441 gccatcggca ctccggcaaa aagcaagaat aacccagtaa gggggacgta ttccgccctg
     4501 cgcttcacca ttaatactga tcccccgcac aggtctgatg acctgaatat taccgttcgc
     4561 ccagtgaatt tccacagcat cacgacagat gtgctgcgcc ataaaaatct gaaagaacat
     4621 atgctttctt ctggcaggcc agtgtcgttc tgaattctcc agcgtttcca ttaaccgctc
     4681 ctgagacctt tcagacattc ctggccggaa atggataatt tcagataagg ctcttccccg
     4741 aatatactcc tgctgtagtg taacctgccg tatggcatca agacactcac gataattagt
     4801 ccggatacgg ggattagcca gggaatccag tttattcagc cctgcatctt caataagcga
     4861 caggagtaaa cggccaaggc gcggacgttt cttgcgaacg gaggagcgct caccattatg
     4921 gtaagcagag cgggcctgca gtctgataac agaattactg tcatcaggtg gtaaaaagtt
     4981 tctgtaattt atacgggtgc taacgggatg ccggcttgct ccgaaggatg tttcctcttc
     5041 atttccggat aactgacgat acatggggca ttcaggtgca tgctcatgat taaaactgga
     5101 cagataaaga gtacccgtat tgtttttaag agcagcaaaa ttcatggccg gcgaatctcc
     5161 cggaatacag tcacactgta accatgcatc atactcacgc atttcccgga gaagatcatt
     5221 ggccaggtta tgttgtgcca tggttgctgg ccttttcagt ccacagaccg tctttactgc
     5281 agtctcttcc tcactgctaa gctcccgaat cactttgccc cggggattct tctttacaag
     5341 tatcattggc ataatgaaag cactccttat gtacatgctt aaacagtaag taaaatatac
     5401 aaagaacccc ccatgaaaca aaggcagaga tgaactgtgc gccccgatat cttcttctga
     5461 tatcctttca aatgaattgt ttttatttat atcatgacaa cattaaagtg agagaatata
     5521 tcaaccatgt ttctgtcagg gttcagtaga atatcaattc gactgtcacg ccataactgt
     5581 tactacctcg agtataaata ctcagagaac ggacttatta gccatgaaaa atcacctgat
     5641 gggaaattta ctaatttatg aattgtgata ttattaaaga gtaaaaccag ccagccgagg
     5701 tatttatggg aaaaaatcag gaagaaagga aaactcccat cattgtggtg aaaaaacgtc
     5761 gtactttttc gccaccgtcc ttatccgaaa aaacagatat tatcgcacct gtatttactg
     5821 aacagacggc agaatcagcc cccgccggaa taaattcatc tgccgtcgaa acacatatcc
     5881 ccgaagcgcc agccaggaag aagaagaaga aaaagaaaag acaccgtttt ccacgtccct
     5941 cccactggac cagagagtat actcacgagt gcgtggaaaa aataaagatc ctgtttcctc
     6001 atcttcgtgc tgaaggtggt ggatttattc ccctgaaaat tggcataaac aatgatattt
     6061 cagcatttct ggcagaacac ccggagacag aactgaccat ggacgaatgg ctctgtgcag
     6121 tgtcctgcat cacctcacgc cgtgtatatc tgcagcggac agctgtcgcc ggcgtcccac
     6181 gttatggtct ggatggtcac cccaaagggc aggtctcaga atctgaagca cagagtgccg
     6241 gtcgcagact ggtgacatta gaacaaaagt tgctcaggat gcaggcacag caggagaata
     6301 tttctgggca ataaacactc ctgataacag ctggacatta cgtatcagaa aaatgggaag
     6361 agaattatta tgagtaaatc aggtgcagaa aaaattcaat tcatcctgaa taatcatgaa
     6421 caggcataca gacgcctgaa gcctgtatat acttttttag aactggcatc ctggatttcg
     6481 gttgttctgc tgattatata cctatcaggc aagaatcacg tgcttacagc agcttttctc
     6541 tgctttgcat ggacagcatt ctgggttctt ttccttttac attatttccc aaaagttttc
     6601 tttattctcg atcgtcagag tctgctcaac ctcatggagg ccaccaaaga ctcaccagat
     6661 gtaaagcagg agctgcttaa ccgccttttt tccggaaaaa gaatgaccgg gcgggatgaa
     6721 atggatatct gccgtcttct gagaaaaaat gaagaagaaa aaatccgcca gagtgaactg
     6781 aatatcatca ggaattttat tgatgaacag cagttaacga atagtgatat tccgaagaaa
     6841 agtacggtcc caaatgaaga taaaaaaggc ataagatgat atgaaattaa taaccaggca
     6901 agcagaggat ggcgtggttt catcctaaga ttgataggag ttaacctgta agataaaagg
     6961 accgtgttta cacgacgtct ctgaagggct tcgtttggga aacggacaat atctaactga
     7021 cgaggaaatt aaataagggt ttaaaaatta acgcaccata tattatacac agtatataac
     7081 gattcagata tcgtcaccag gatatgccgc gccagcggca tggaaggcgg cacgctgtgg
     7141 ttacatgtga taccggagta aaaccgccga agcccggcgt cagccggtac tgattgatag
     7201 atttcacctt acccatcccc agccctgcta agccataccc gctttcagcc atgagagagt
     7261 ttctgtgcgc ggtcggagtg gtcccgacga gggtttaccc gaagtcgggg cgtatctccg
     7321 cgttagcggg ccgtcagggc cgcttacgag cgtgtacgga ggacttccag ccagaagact
     7381 ggcagcgatg atgctgtgta gttacaacat tcataattaa aagcgatgtt gttcccgccc
     7441 tttgggcggg ggcggcctcg cttttcagtt atgaggggtg agcaggatgc tgcgcttgtg
     7501 tggtgttttt attcctggtg tctttttgct gtggtttcag aatggtgagg ttcggggccc
     7561 gcgcgttagc gtgggaggta cttttaaatt ctgagcggcg cggccttggc cgctgcaagc
     7621 gaaggccttt ttgtatttgt tcacagtggt ctcagagata aagactttcc ctgcggcgaa
     7681 gcaacgcagg ggattaaatg atggggttaa gaatatattg caggtttgat gtttcaggtt
     7741 tgcagcttct taaagttctt tgtttgcgga tttatgtgga taactgaaac aacgtggcca
     7801 cgttctgatg actgatgcgt ggttttatgg gcagatatgc ccctctgagg ggatttaagg
     7861 tgactactaa ggattttggg ctgagcggaa tctgcgccga aagccccgga ggggctggcg
     7921 atgattcagt gctgagggaa tgatgctgtc tagtgcggtg gcggggcagc cagcgtcagt
     7981 tcaagctgac gcaactccct gatggccagt cgcttcagat ggtcatcgga acagaaatag
     8041 gcttcatccg gcggcagacg cttccggaga tactcagcca tctcataaat ctgctggtca
     8101 acaggtaatt tcttcaggcg gttggcacgc ttgctggctg ccgctttttc acggcgcttc
     8161 ttcagggcat cctggctgcg ctgcagatac cagcgttttc tggccgcatg aacggagata
     8221 tcttcatcct cacgcagaac accttcccga atgagctgct ggcggatttc actttcacgc
     8281 agtcgtttct gctgctgttc gtgaagtttt accatgtcga cgcccagcat ctgccagcct
     8341 gccggtgtta tccagacgta acgtggcaga cgctgacgat gttcacggtc ccacattgtt
     8401 tcctctgaca cacccagcac accaaagcgc acctgttcgg ccagcagacg ggaaagacgg
     8461 gagaccgtca cttccagttc cggaataaca tgtccctcgc tgtctttcgg gctgatttct
     8521 tcagccagcc gagtgacgct catccccacg gtatgtgtgc cggcatcact gaagctgacc
     8581 agaaccggcc agatggcatc gaggagacgt tgtttttccg gccggaaagc gtatttgcga
     8641 ccgacgaacg tccgtttctg tacataaaaa ggatgccgcg acatgcgcat gcgcaggctc
     8701 tgctcgccgg ttacagggtc ctttttcacc agacggttat aggcgtgccc gagctgccct
     8761 ttcagcgctt tatagcgctc aggacgatac caggacggat tttcgcattt gggtttagtg
     8821 gagtgctcac cacgacggcg atttgtgagc ttcctttccg gttcaaccag gaaagtggtc
     8881 gcccttcccg cttcatagtc ttcccagaaa cggatctgct cttcgggtgc taactgactc
     8941 cagtgtactg cgttatatgg agtattcttc agacctgcca caagctgtcg cttatggcgg
     9001 agcgattgtg ccgtgtattt atacactgca cagggttgaa tctctggtac ggtttcatta
     9061 tactttaccc gtattctgtg aggcccccac tatctttcgt cgttccgcca agttcgtaag
     9121 aaagatagtg ggggttttcc ttttatggct tccggaacat cttcagtctt ctgacttact
     9181 gcccgtacat gccatctctc ctgaatgcct tatggcgcat tctgccgtcc ggctttcgcc
     9241 gctttgctcc acagagtgcc accctctgca cagctaataa cagccgaaat atacccgctt
     9301 tacgttaatt tgaaaagcat tacaacgatc tcttctcttc cagtgcctcc tcactgatac
     9361 ctctaataag ttaatcttga cctgcgtcaa aaaaataaaa tctattttta aaagcagatt
     9421 tttctgcttt agatctttct tttggattaa aagatcgact tttgtcctac tcacgatcat
     9481 ttatcttaca accgatctac ctaacagaat aatattctgt tttaacgcaa ttaaacgatc
     9541 taaaaaacct ttaaaaaatc attttgtaga tcattcgatc gtaaatagaa taattcttag
     9601 ataataaaac catttgttac acagcccgcc aattgtgtaa caacagcctc tacaagagca
     9661 tatttgttca tatgttcgtc agaactgcgg attgtctgga cgagggtcag acaccgtttc
     9721 gtgcatatgg accagcatgt tcgaacgcca caaagcagaa gcatcatgcc tggatcgctt
     9781 cggcgcggag cgttaagttt tgacgctttt ttgtatgtct tttaagcggt tagttgactc
     9841 ggtacgttat actaagtacc ctttactggt cacaggcaaa actgtgatcg ggcgtaggaa
     9901 cccgcgtagt catcgtcggc gcagaagtta atttcttcat ttggcgtcgt tttttgccat
     9961 accataaaga caggaacttg tatttcttgt cgtcatctat ggtagctcag gcggggcagg
    10021 cattagcctg gccggtttcc gatgacgccg gtattcctac ccccgtctgg gctatcacca
    10081 atgagcgtag gaactcggtg atagcgtttt tagttgtcat cggagaccgt tgctatgtca
    10141 cctcaaatga tacagaaact cccggccatc actctgttgg aaggcatgtt tcccgagcta
    10201 tcaacaaacc agcttaaagt ctgcgttttt tacgcaatgg gtgttcctta cgacgccatt
    10261 gctcagaact gtcggctttc tcccgaaacg gtacgaacct acctgaagcg aagcctgaag
    10321 aacttaaatc tggaaggata tgatgcactt cgctctgctg tgctgatgcg aacgttcgtt
    10381 ttcatgatta gcaatacagc taaagaaaac gaaaaaatgt gaacaggtgt ccccattaaa
    10441 ggggacactt tcatggaaat tgcattttgc actttacagg cgatcccaaa ttgataaaca
    10501 taaacaccaa gcacaagtta ctttaataaa gcaaatcact tcagacggtg tcccagttat
    10561 ttgtatctgg ctcatgcccg atatatggcg accttactct gaactgacgt gctgatgttt
    10621 tatttcgcgt atcacaatac tgtgccactg tattgctgat attagtgctt agccgttctt
    10681 tatctgtgag ttcgccaaat caccgataca gaacgtacta gagtctgctg gttatatgcc
    10741 tgtcagaact gcagtttatc cagatatggg ctggacactg cttcagacat atgatttagc
    10801 atattcgcct gacatcgcaa aatcgtccat catggatcgg ttttgcgcgg agcgttgaac
    10861 ttcgtgaata tagaacacct gaataaccgg aactggtatc ttgcgcaata taatacagcc
    10921 ggcaaaaaca gagaatcttt attctcatgg ctgaatgagc aaaacgtcgt accatggact
    10981 ccattaataa caagaaagat aagacgggca gactccaggt gctgttaccg tgaaagaata
    11041 ttcgctattt ttccagggta cttttttatt ctggctaatt ttgacattca cccagtatca
    11101 gcattacgtc gacatagcgc cttcattgat ttcgtgaagt ttggcggtga aataaaaccc
    11161 gtcaataaag atattgttga tggattaatg aagatttacc cggaccctgt gctaaatccg
    11221 ggagccaggg aagagctgaa tgcggcttcc agcatatggc tgacaaaagc acaatatcag
    11281 tatctcctca gaatggaaaa tactctccag cctgagtcga ggatatccct gctcctggaa
    11341 ctggtatcaa atgccgaaca tcacggcttt atggagcgcc ttgtaaacat cccatagcca
    11401 gtatttcccg gccttcgggc cgggcttttt tcaattccag agaatattcc ggagtgaagc
    11461 ctgcacgaag ccatccccac catggactcc ccctgtcttt tgatggaacc gtgtatttct
    11521 taatggcgaa agaaataaaa ggcttcaccc cagagtattg attcgtttta tcaaaggccg
    11581 gcccgtgcca tcacgtgtgg cggagagaac ggataccggt cttcttttca tctcacagta
    11641 ggacattcgc atgaccacga cagaacatcc cgtgcgccag ttgctctggt gtgcactgaa
    11701 tgccctgaaa acggcacagg aaaaccacaa tataaccagt gaaaccggat tacggcatta
    11761 cctgcttgaa tggctttctg gtgccagcag acatcctgag ttccgtagtc tccctggcga
    11821 aatcatggcg ttaaaagcat tgaccgaaaa agatcgcaat attcccatca tcggaacgct
    11881 gaatacgctc tttctcagct ctgccactgt cggggaatgt ccgttgtttc gttttcgggc
    11941 tgcactgggt aaattacaga aagcaggctg gcgcacatat gtctgcccat ggcctgagag
    12001 ggtgttcaat gaatccattg aacgggcctg ttccgggaaa cgtcatctgc tacaactaag
    12061 tcgcaccgaa gagtgttttc taccgacagg cgatatgagg gcaccagtta cgttgcagct
    12121 gatcactcca ctacaccgca aaactatgca gcttctggaa acggcagagt taatattctc
    12181 cgacgaagga tttcaggtgg tgagaggata cgaacatcaa ttcggggtcg aacgcagaga
    12241 aaccctcttt cacaccctat ttatcgggct gcccagcctg ccagaagatg tctggggtgc
    12301 aacacaggag cagacgacta tcacattaca ctgaggatgt gtaatccccg gcataccccg
    12361 tttccagtct tcgcagcctg taccgccctt cctgccgttt ccgacattct ttctgtcact
    12421 actcgcttgt gccatttttt cgcaagcggc ccccatcgac atgacggtgc gagtctttct
    12481 ttttaccctg agaggaaatt aacagcatga aaaaacaacc actactctgg atgacgatag
    12541 ccgccgcact gctgtccggc tgcacaggcc acagggaaca tcacccgcag gtgacattca
    12601 cggtcgatac cgtgcgaaca gacacaacgg ccagcaaccc aatctgcctg gcggaaaaat
    12661 acggaagcct tcttaatgag cgccatccgg acctgtttgc cggtgctcag gggagtattt
    12721 cacgcagtgc attttttaat gacagcctga cgctgacacc tgaaacgctc cacgggatga
    12781 tacagaatac gccctgtgtt gaatcactgg taacgtccgt aggtccggat accttcactg
    12841 cagatgtctc tcccggtgtc agaatgcagg tatcaccgga tattcacggt gcgcccggaa
    12901 atatcactct gcgactctcg accacagacc agaaacgaaa acagactgtt gaacaaagca
    12961 tttctctcag accggaacag tcccttgtcg tgaatggatt caccggagac ggagctggcg
    13021 aaaagcgcgt cgttctcatc acacctcacg tgcgttaatt cccaacacaa cccgtttccc
    13081 gtcttcgcag cctgtaccgc ccttcctgcc gtttccgaca ttctttctgt cactactcgc
    13141 ttgtgccgtt ttttcgcaac cggtacgtgc agttatgacg gtacagctgc ttcttttccc
    13201 gttccggaag gagtctttga tgccacagca acatcccgga cgactgcaaa tactggtcgt
    13261 cgacgcacac tgcaaacgca ggctgttttc aacgaaaaca ccgacagacc ccgatgagct
    13321 tgcccgacgt ttctgcacgc cggataactg tctcgtggtg gtactacgcg acaaccgatt
    13381 tctgttccga ctggagcgtg ctcccggcag ccactgccgc tggcacaagg gcagcagctc
    13441 ccgccatcag catctacagg actggctgtc gtaaacacaa caaaaacaaa agacctcgtc
    13501 aaaacaggtg ggggccgtgt tctttttagt taaattaacc atcgcgattg cgattgtctg
    13561 tgtacacctt ttaacgattt taatttagcc gtttgttgcc atctttatta cctctctccc
    13621 ttttatcatg gagattaacg atgtttacca tgttctttct agttaaatta accatcgcga
    13681 ttgtgattgt ctgtggaatc ctttctccga ttttagtttc accacataac aaactgtcca
    13741 cacttttttg gaagatacca aacgcgttct ggattttcct gatttgcata tactttgcaa
    13801 tcgccatggc ggtgttaata gaaagcagtg aacagacacg catccgccgc caacaatatt
    13861 tccaacagta cgaagtgcca aatgagacac ataaaaatgt ggtgatagaa aacagtatta
    13921 aaagtcaact aatatgaaaa ataataacgt accattatat ctatggttgc tttggtgtac
    13981 gctttgtctg tacatcacta ccccatggca atacttacgt tatccataca ctgacaactt
    14041 atcattgcct tatatgttat atttctgtgt ggtggttgtg atttgcattt tcccactact
    14101 tttcagatgt gtacttctta ttgtaaggtc tgcatctaaa tacgacttca cattctttgt
    14161 gaagaagaaa aagggaagca agaaatcaac tatacatctt agcccctatg ctgacacacg
    14221 ttgcccgaag tcaatgagga aacactggga tgtattgctt tctctgatat caaaagcaat
    14281 taataatgat gaaatcattg tcatgcattc acatctcttt actccagcaa gaataaataa
    14341 acttataaag agactggaat cttatgattt gagtttttca gttattacat acccacggaa
    14401 aacaagtctg tacgaaaaat tatcaattcc ttttatatat ctcttatttc agtggtcttt
    14461 cccgcatttg cattcggacg gaatgacagt cgtcattcgt cctctccagc cgcctcacac
    14521 caattaacaa acacgtatat acgaacacgg agtctccgtt atgaaaaaaa actgtctgac
    14581 acttatactg ccgtcattat tgtccggctg tgccggaatt tccggcagca ataacgtggc
    14641 accatcagat acgcctgctc tggtttttgt tgacggacaa atcagtgaaa gtgctggcgt
    14701 tctggcactt acgcaacaac gtctttctcc acctaaaata actccccccc cgagagtcag
    14761 cgtgccagag acagtaaaaa cagcacagcc tgttagcgaa tcccgaccgt taccttcacc
    14821 aacaataagt cagaatttct cccggttaat cattacaggc aagcctggta gcgcccctgt
    14881 aacactatcg ccgacggccc gtaaccggac cgtcagtcag tggataaaaa cattactacc
    14941 ggaaggatgg aaattcagac aagagaatgc agccacgccc aagctgaata ccaaactggt
    15001 ttcatggtct gccaatgatc agtggacccg ttctctgaac cggttgctgg aagaacaaca
    15061 cctatggggc catctggact gggataataa aacgttgacc gtcacaacat cagctgctgc
    15121 accggcacag gacacggcaa ccactatctc gccagcagcc atggtaacca catccacttc
    15181 ccctgaatct ccaacaacag caaatagcca gaacaaacca cgaaatccat tcagaggaaa
    15241 cagcgtatct tcgtcaaccc ccgccgccac cccaactcca accggctcca cagtcaagtc
    15301 tatcccgctc atgaccggga cgcctgtcaa accggtatct cagggtaaag agtggcgtgc
    15361 gccggcaggc accacattaa gagagaacat tattaagtgg gcagaagaaa caaaatgcga
    15421 aagcatggca tcaacgcact ggatggtcat atggccactt tctgtaaccg attaccgtct
    15481 ggatgcgcct ttaatgttcc ggggatcatt tgaatcagcc atggagcagg tatttgagtt
    15541 atatcggact gcgcaaaaac ctttatacgc acaggcttct cgcatgcagt gcatcattac
    15601 agtttctgat acaagggatg gtcgctaatg ggatggctgg tcatggccgt cggcctgacc
    15661 attctgatta tcactggtag ctaccagaat cagaagatgt ctgaaaccac aaacgcccag
    15721 caatatgcaa gtgcgtctgt atgggcaagt cagatactaa tgattgctaa cagaatcaat
    15781 gatatcagat acgtatcagg ccagcaggac ggagtaattt cctcagataa actggctctc
    15841 cctgtcactc cggactcccg aattaaacat cagttacaac aggggcgtct gtgggtatgg
    15901 atgcctgaac aaccaggact tgttgaaact ttgcgttcaa aaagtcgtgg ttctgcacta
    15961 atagggatct tccaaaacgg gcaactcacc tggctatcag gaactgcaac cggtctgaca
    16021 cccccggcag gcattactgc tggttctgtc gtctacgtta actaatctgg atatattcat
    16081 gaaaaaatca caccagcgtt caatgaagct ggcggtgctc ccctgcatga tcgccgtcgc
    16141 tctttccatc tccggatgta ctttcagcga aatcaacaaa atgcagaaaa aagcacagga
    16201 agactcagca catgcacggg aaaaggtatc agccctttcg gcccgtaaat cgcaggctct
    16261 cacctggctc gataatcaat ggataaaccc tgttccggtc gctcaggtat caagagagaa
    16321 aaaacaaaca gctccggcct gctacatcac gcaggcaaga aaaggagaga tcactctgca
    16381 ggaactgggg caacgtatta ctgccgtatg cggcatccct gtaatcatca cgcctgacgc
    16441 agccaattca actcttgaag gaggcgctac ccgccagatg acaggaacac taccagcacc
    16501 agatgaaaat ggacgtctac cgttaagcag cctgggcagc acaacaatga ctacctccac
    16561 tcagccatta acgctgaata acctcatgtg gcagggagat atcaatggtc ttctggatct
    16621 gatggccagc cggagtggtc tgtactggcg catggataat ggtcggattg tattctatct
    16681 gactgaaacc agaacgtatc cacttcatat gctgaacacc aaaaccagca gcagttccag
    16741 tgtcagctct ggctcaacaa gcacaatggg ggcaacagga ggccaggata actcagcatc
    16801 cggtgatgca acgtcctctc agagcacaac cgttggtcag gaatacgatc tgtatgaaga
    16861 catccggaaa actattgaag caatgctgac accagaaaaa ggccgttact ggttatctgc
    16921 atcgagctca acgctaacag tcactgatac tcctgctgtc caggaagccg tcgcacgata
    16981 tgtggacgaa caaaacagta ttatgaaccg ccaggtagcc ctgaacgtac aggttctgag
    17041 cgtcagcaat accagaaacg agcagttcgg tctggactgg aaccttgttt acaaatcgct
    17101 acattccgcc ggagcaacgt tgaacaatgc aagcggagat tttacaggcg ctacatctgc
    17161 aggcgtatca attctggata cggcaacagg gaatgccgcc aaattcagcg gttccagcct
    17221 tctgattaaa gcactgagtg aacaaggcga tgtcagcgtt gtaacttcac aagaaagcac
    17281 tgtcacaaac ctgacgccgg tacctatcca gatggcagat cagacagttt acgtcgccca
    17341 gtcagcaaca actacgacta cggatgtagg agcaacaaca acattaacac cgggcatgat
    17401 caccactgga ttcaatatga ccctactgcc tttaattcag aaaacaggca atctccagtt
    17461 gcagatgaac tttaatctgt ctgatccccc aactatccgt agctttacgt caaaagacgg
    17521 aaacagttac atcgaaatgc catataccaa actgcgttca ctgagccaga aggtcaatct
    17581 gaaagaaggg caatcacttg tcgttactgg tttcgatcag aacaatacga cgacaagtaa
    17641 agccggtacg tttacgccag caaatccatt atttggtggt tcacaaaccg gaaaaaatga
    17701 acgcagcacg cttgtgatca tcattacccc gactttcccg tcaggaggca ataatggctg
    17761 atgaagatat caacccggta gtgcttctcg aagatcctaa agtaaatcat cgcgtgtggg
    17821 ctgcctgtct taaatggact cctgtcgtaa aaaaacagcg cgtcccctca catcataaac
    17881 acaagtctca tgtaaaaccc agacggctta cctccctgaa agtgactgta ggcagcagaa
    17941 attcccgtgg aaagatcagc cgccttaccg gcacaggaat actgaccagg ccagaacgca
    18001 atcattattt ttccctggca ctggctttct gctcatgggt cagaaatgga tacggtgtct
    18061 tccggtacag cgataaggag ctcttattcc ttgcttccat taatggccag cctgccgtta
    18121 tggctgactt atcaggaaat gatgccgacg ttgcccaaaa ggtcagcctt tttctggcga
    18181 tgaacgaaga gccccccgaa aaatggcagg ttgtatcgtc tctggaacat cctgataact
    18241 gggaaagtat tattacccgg ttatcatcag cagacctccg ccgttgcaaa cttactgtcg
    18301 gcaatcgcag caaattcaca ctgcctgccg tactgttttt agtagcagcc ggcgcaggca
    18361 ctgtattctg gatgactcag ccagagccgg atgtcgtacc gacgcccgag gagatagctg
    18421 ctcgggcaag attgcagttt aaaaagcccg atccaccgcc agaactcccg catccatggg
    18481 catcacaacc tgtaatcagt gattttctga aagcctgcgc ggaccttcga aaaccgtcac
    18541 cggtcgcact ggaaggctgg aaacttaccg gcggaacatg tactcctgag acattcacac
    18601 tgatttacga acgacagcct ggaggaacca ttgagggttt tctggcaaga agtaaagaaa
    18661 tctttggtgt tattcctgac tttaacctta aagatggtgc acgccaggtt tctgtaaccc
    18721 gtccactgcc gtccctgccc ggcagggatg aggctgtccc ggccccctca gaacaactga
    18781 tgcgggtgtt cacgtggttt cagaaaaaac agttaacacc ggccattaat gaaatagcca
    18841 taccggagcc attaccaggc aacgatggtg aaccagcccc tgttcagaaa tggaaagaat
    18901 atcagttcag tttatcaact cctgtaaatc ctgatgagtt tttcccttta cttcaggata
    18961 ccggcgtccg tctgagcaat atccattttg aattaaacgg cggtactttc agttattcct
    19021 cagaaggtca tatatatgcg tccaagtaaa ttactcatta ttccttccat atttttcttc
    19081 tctggttttt catttgccac aacacaacct cttgtcacaa taggagaact tgaagcacaa
    19141 caaaaccgga atattctttt acaggcaaaa gttcagggtg cacagttaca gaaacaactt
    19201 gaagaatcag atgttacttc atcatctgaa actgtttcag gtttctctgg tgtgaccgcc
    19261 cccttgccat cagtttcaga gcaaacggca ccgaaaaaca gaaaagaatt gcccatcatt
    19321 atggaaataa acggcaaaga taaacgtctt catgccgttc ttcgcatggc tgatggacgt
    19381 cagacaagcg tcaccactgg cagccagctt ccaggtacat ccgtcacggt gaagtcgatc
    19441 tcattgtctg gcgtcacgtt aagtgatggc actaccctta ctttctgagt aacgtgatat
    19501 ggataaatat aatctgaagg atgctctcct ttttatatcc cggggagaca cccatgaaat
    19561 tcttatagaa acaaaccagc ggacaaggcc tgacgtacaa agtaatctcc aggaactgct
    19621 aaaattatat ccgggtatca acccaaaagt agtctcgttg tcggagttac aggaagcagg
    19681 tcaggatgaa aaaaacagag gcccgaaaga acgaattatt catctgaaag atcttgctga
    19741 tgtcagtgaa agtgagaaga aagttctatc ttattttgaa acggccagaa aacttggtgc
    19801 ttcagatatt cattttttaa tttctgagtc tattttcaaa gttcgcatga gaatattcgg
    19861 tgaactgcag actgtagatg aagatcaacc agcactgggc tattcacttt gcgcaacagc
    19921 cattctgtca atggctgatg tcactgaaac cagtttcttc cctcaaaggg aacaggatgc
    19981 gcgcctgtcc ccacaattaa tgcgaaaaat aggaatattt ggtgcacgct acagtcacag
    20041 acctacggga gacggtctga ttgcagttat gcgcctgata cccgatgacg gagataaagt
    20101 tcccacgttt aaacaactgg gctttatccc ggaacaaatc cggttgctga acataatgct
    20161 gcgccgcccg gaaggaaaaa tagtcctgtc aggtccaacc ggttccggta aatcgacaac
    20221 gttacgcagc gcctgccgtg tatatcttga cgataatcag gggcgacacc tgctaacaat
    20281 cgaagatccg ctggaaggac agatactggg agcaacacag actccgatta tctgcgacaa
    20341 atccgacgaa gatgccgtca aactggcatg gagtcgggct atttcatcgg caatgcgact
    20401 tgaccctgat gccatcatgg aaggggaaat gcgcgatctt atctcgatga tgtcaaccac
    20461 ctacgcggca cagaccgggc acatagttct gacgacatta catacaaact cagctctggg
    20521 tataccggag cgaatgatta cgatgggtat gaatgccgat cttatttgcg atgcgcaatt
    20581 actgattggg atgatcagcc agcgccttgt accaactctg tgtccctcct gccgtattcc
    20641 atgggaaacg cgagcacctg aacttagaga cgacgagcgt gattaccttg agcggcactg
    20701 caataaagat tcactctgca gtacagataa tatctggttc cgaaatcctc acggatgctc
    20761 tgaatgtaat cacgacgtaa tcattaacgg cagaaagcgc ggtgaaatag gtaaaggcct
    20821 caccggcagg actgtaattg ctgaggtgat agaacctgat aaccgcttat ttcagattct
    20881 gaaaacacgc ggcaaagttg cagcacgtaa atactggctg gaaaacatga aaggcatcag
    20941 tcgtgtagag catcttcttc gccgtatcaa tgaaggtctg gtagaccctc ttgaagccga
    21001 cagaatcatc cctctggacg aagacgagcg cctcagcatc gatgatgtct aacaggagaa
    21061 caacatgcgt gaaatgaatt tcagtcaacg gctgaggcgt ttcattgtca gaaaaacgtt
    21121 cagcgccccc tacagggtgc agttttacga agccctgcgt tttctgctgg aaaataaaca
    21181 gccattaaag accgcactgg aacagatgcg ggatgcctgg acagactttg gacgaaaatg
    21241 gcatcccttt gctgaactgg ccacagactg cattgagtct cttcgtgaaa acagtggcga
    21301 aaactctctg gaatatactc ttagtttatg ggttcctcag gaagaggccg cagtaatcag
    21361 cgccgggata agaagtggtt caattgtgga tgcactgcaa ttcgccacca ctcttacaga
    21421 tgcaaaagaa caaatccatc aggctatatg gcaaatggct atttatccgg tgggattgtt
    21481 aatcatgatg acggggacat tatatgttct gaatacacag ctaatacctg aactgagcaa
    21541 aataagctcc cccgattcat ggagtggtgc tcttggtttt ctttatggat tatctgtttt
    21601 tgtcgataat tacggcgcta tatgtgccgt tctttttgca gtgatcacag gtttaatatc
    21661 atggtctctg ccaaactgga aaagccctga cagtgttcgt acttttgccg acaagataat
    21721 gccatggagt atttatcagg atatacaggg agcaacattt ctcctgaata tggctgcact
    21781 tctgaaagcc aaaatgacga cgctcaactc actaaacatt cttcaggagt ttgcatcacc
    21841 atggttaagc acacgtcttg acagcattat ttaccgcgtc cgccaggggg atcatcttgg
    21901 actggctttg cgtcagtgcg gttatcagtt cccatccagg gaagcagcca acttcctgtc
    21961 tttattacag ggagatggcg caacagagct aatcagcaat tatggtcaac gatggcttag
    22021 tcaaacactt caacgtgtaa agaaacgggc aaacgtgatt cgtttaatta tgctgatatt
    22081 tcttgtcatg tctctcatgt tactggtttt tgccattatg gatattcagt ctattagtga
    22141 caacagcatg ggtaactttt aaacaaaacg aggatattta tgttagtaga aaatattaat
    22201 gccactttaa ctggaaataa taagaagaat gagcctcatg ataaaggatg gggaattttg
    22261 gagcagggaa caatagcgct cgttgttctt tttgttatag tcgttgttct gggtagtttg
    22321 tatgcgttac gtacaagaac caatgtggca actgaaaccg caaatataca aacaattatc
    22381 acaagtgccc agagcctgct aaaaggtagc gatggatata catttaccag cagtgccaaa
    22441 atgacaggtg ccctcattca gatgggggcc gttccctctg ggatgacagt acagggcgac
    22501 aaaacatctg gtacagcaac gctctataac gcatggggcg gtgctgtaac tgttgccccg
    22561 gcttcaacat cagggttcaa caacggtttt accgttacct atgataaagt cccacaggat
    22621 gcctgtatcc agattgccac acggatcagt aaaactggtt taactaacgg cattaccctg
    22681 aacagtactg ctcacagtga tggaaaagtc actactgaag aagccagtac gcaatgcaag
    22741 gcagataatg gcagcactgg tacaaacaaa cttatcttca ccatcaatgg ttaaggagtt
    22801 ttaatgtcag ggggtacagt acccctcaga cttatttcat taatcctgct tttttctctt
    22861 attccacccg cccgagcttc atacgtagcc cccaaacaga tctggaccaa caagtgggac
    22921 aattgctttg ccgctgccgg cgcgcgctat caaattgaac cattattatt gaaggctatt
    22981 tctgccgggg aatcttcgtt gaggccgggc gcaattaata taaataagga caaaaaaaca
    23041 ggtaaagcca gtagcacgga ttatgggttg atgcaaataa actcaaccca tattccaaag
    23101 ctgataaata tgggcgtaat aaaaaagtct gaggatttaa tcaccaaacc atgccttaat
    23161 atccatatag gaagctggat actggcaaga cattttcaga tatgtggcgt cagctggaat
    23221 tgccttgggt catacaatgc cggattccgt aaggacagac atgaaacaag agagcaatat
    23281 gccaataaaa tatggagaat ttatcgtgac atgaaaggga tatgtcttcc cggacaggga
    23341 ggcaggcaat gcagacaatc gtaggcataa gtctatcccc tgtttatata ctgccattga
    23401 tgttcacatt ttccattatt ggacgaactc tgctattcag agttcgttat tttttatctg
    23461 atacgggaca tctctggtat aagacacacc ctgctgttct gtcaggaata tggctataca
    23521 gtatagctgt ggcgttaatt attttatcat catcaccact gctctaccgt attcatgctg
    23581 tccttattct ttcgttcata ctgcaaatgg cggtaacgga tgcactgaca ggtttattac
    23641 caggaacatt cacccggcgt tttttaattg caggaatgct atcacaaatc acgactgata
    23701 tatggtggtt ccgtacaaca gaatttgcca ctgctgcaat tgtcttattc tgccttcata
    23761 aattagttaa ccgccatcgt cttaatattg gcactggaga cctgtggctt attgcaggta
    23821 ttaccgcatg gtcagggtta tataacgcca tatggtgtgt gttgctgggc acaggaggat
    23881 ttgtcttatg gcattcaaca tggtgcataa aagggcataa agaaggccct ctgggtccat
    23941 ggctatgttt tggccatgtc cttttgctgc tggataatct ttatcaacca ctatgggtaa
    24001 tctgatgaaa aaatatgacc gaggctgggc atcgctggaa actggcgcgg ctttactgat
    24061 tgttatgctc ctgattgcct ggggagcggg catatggcag gactatattc aaacaaaagg
    24121 atggcaaact gaagcacgtc ttgtcagcaa ctggaccagt gcggcccgct cttatatagg
    24181 gaaaaactac acgactctcc agggcagtag taccacaaca actcctgccg ttatcacgac
    24241 aaccatgctg aaaaataccg gctttttgtc cagcgggttt actgagacaa acagcgaggg
    24301 gcagcggtta caggcatatg tggttcgaaa cgcccaaaac ccggaattac tacaggcaat
    24361 ggttgtatcc agtggtggca caccttatcc agtgaaagca cttatccaga tggctaagga
    24421 tattaccact ggtcttggtg gatatatcca ggacggcaaa acagccacag gtgcattacg
    24481 ttcctggtca gtagctttaa gtaattatgg tgccaaaagc ggtaacgggc atattgccgt
    24541 attgttatcg acagatgaac ttagtggtgc agctgaggac actgatcgtc tttacagatt
    24601 ccaggtcaat ggtcgccctg acttaaacaa aatgcacacg gccattgata tgggatcaaa
    24661 taacctgaat aacgttgggg cagtaaatgc ccagacaggt aatttcagcg gcaatgtgaa
    24721 tggtgtaaat ggcactttca gcggtcaggt aaaaggcaat agcggaaact ttgacgtaaa
    24781 tgtcaccgct ggcggtgata tcagaagtaa taatggttgg ttaattactc gtaacagtaa
    24841 aggctggctc aatgaaactc acggtggcgg attttatatg tccgatggat catgggttcg
    24901 aagtgtaaac aacaagggca tctataccgg cggtcaggtg aaaggcggta ctgttcgggc
    24961 tgatggtcgc ctttatactg gtgaatactt acaactggaa agaactgccg ttgctggcgc
    25021 atcatgttcg cctaacggcc ttgtaggccg cgataataca ggggcaatac tttcgtgcca
    25081 atccggtacg tggaagactt ctggttcgct caatggttct tacacaaact taggttcaca
    25141 tagaggttca ttctcagggc ggaattcagg gggcagtaca ttgtttattt atgcatctgg
    25201 aggtaatgga ggatctgccg gaggtgcatg tgcaaataca tcccgactgc agggatatgt
    25261 tggtgggacg ctaattagcg tgaatgccag caataaccct gcatatggaa aaacagcctt
    25321 tatcagcttt gctgtacctg caggtacttc ctatcagata acatcctatc caacagaaaa
    25381 tacatcatgt ggtgccgggg tattttcagt atttggatat caaacttaat tgagcgttac
    25441 acacgacgca atacaacctg tcttatgctc gtatttgtac catttgcgtg aacctgctgg
    25501 gctctcaact acctggcagt agtgggcatc ctctgcgttg cccattctgg caatggcaca
    25561 gaagtcgaat tgccccagat aacctgtggt ggaaagctga gtaactttga gttttccacc
    25621 tattgtc
//
LOCUS       DAEBXN010000057        24868 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.057, whole genome shotgun sequence.
ACCESSION   DAEBXN010000057 DAEBXN010000000
VERSION     DAEBXN010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24868
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.057"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            167..1666
                     /gene="pitB"
                     /locus_tag="KJL99_002378"
     CDS             167..1666
                     /gene="pitB"
                     /locus_tag="KJL99_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HBD4437082.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPIDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(1715..2407)
                     /locus_tag="KJL99_002379"
     CDS             complement(1715..2407)
                     /locus_tag="KJL99_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HBD4437083.1"
                     /translation="MQSITPPLIAVIGSDASGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            2581..3294
                     /locus_tag="KJL99_002380"
     CDS             2581..3294
                     /locus_tag="KJL99_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HBD4437084.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGCYLRSKAAHVHEKPLTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            3326..4084
                     /locus_tag="KJL99_002381"
     CDS             3326..4084
                     /locus_tag="KJL99_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4437085.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERSLYQKMAQYRPELIIRLGIDIDTAISRKPDHEYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQVQKAVSLVAIVSDRRSLT"
     gene            4130..5479
                     /locus_tag="KJL99_002382"
     CDS             4130..5479
                     /locus_tag="KJL99_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HBD4437086.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVESALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFVGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            5479..6303
                     /locus_tag="KJL99_002383"
     CDS             5479..6303
                     /locus_tag="KJL99_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4437087.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYYAPLALDSDICQRLELLISRFPL"
     gene            6315..6875
                     /locus_tag="KJL99_002384"
     CDS             6315..6875
                     /locus_tag="KJL99_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HBD4437088.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            6906..7976
                     /gene="lptF"
                     /locus_tag="KJL99_002385"
     CDS             6906..7976
                     /gene="lptF"
                     /locus_tag="KJL99_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HBD4437089.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRAGSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLILVARDFSLLQKFSR"
     gene            7973..9052
                     /gene="lptG"
                     /locus_tag="KJL99_002386"
     CDS             7973..9052
                     /gene="lptG"
                     /locus_tag="KJL99_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HBD4437090.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDGVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYHLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(9088..10259)
                     /locus_tag="KJL99_002387"
                     /pseudo
     CDS             complement(9088..10259)
                     /locus_tag="KJL99_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            complement(10259..10507)
                     /locus_tag="KJL99_002388"
     CDS             complement(10259..10507)
                     /locus_tag="KJL99_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBD4437091.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(10540..11454)
                     /locus_tag="KJL99_002389"
     CDS             complement(10540..11454)
                     /locus_tag="KJL99_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HBD4437092.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVRQLAAKARCGSVRMVDIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            complement(11451..13181)
                     /locus_tag="KJL99_002390"
     CDS             complement(11451..13181)
                     /locus_tag="KJL99_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HBD4437093.1"
                     /translation="MVYMSNKIFTHSLPMRYADFPTLVDALDYAALSRAGMNFYDRRC
                     QLEDQLEYQTLKTRAEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAV
                     PLAIPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWF
                     KALPEADVALQRPVPNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPS
                     DRCVSWQPFYHDMGLVGFLLPPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVA
                     PPFGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFM
                     PCYGLAENALAVSFSDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALP
                     EHGIEIRNEAGMPVAERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLL
                     DGYLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCR
                     ISDEERRGQLIHALAARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYA
                     ASLNVQESLA"
     gene            13543..14684
                     /locus_tag="KJL99_002391"
                     /pseudo
     CDS             13543..14684
                     /locus_tag="KJL99_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
     gene            complement(14808..15578)
                     /gene="glcC"
                     /locus_tag="KJL99_002392"
     CDS             complement(14808..15578)
                     /gene="glcC"
                     /locus_tag="KJL99_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HBD4437094.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSHVARLNREQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENHKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNKVGM"
     gene            15829..17328
                     /gene="glcD"
                     /locus_tag="KJL99_002393"
                     /pseudo
     CDS             15829..17328
                     /gene="glcD"
                     /locus_tag="KJL99_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
     gene            17328..18380
                     /gene="glcE"
                     /locus_tag="KJL99_002394"
     CDS             17328..18380
                     /gene="glcE"
                     /locus_tag="KJL99_002394"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HBD4437095.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVVGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            18391..19610
                     /gene="glcF"
                     /locus_tag="KJL99_002395"
                     /pseudo
     CDS             18391..19610
                     /gene="glcF"
                     /locus_tag="KJL99_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
     gene            19615..20019
                     /locus_tag="KJL99_002396"
     CDS             19615..20019
                     /locus_tag="KJL99_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HBD4437096.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKVAAAVLAK"
     gene            20041..22212
                     /gene="glcB"
                     /locus_tag="KJL99_002397"
     CDS             20041..22212
                     /gene="glcB"
                     /locus_tag="KJL99_002397"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HBD4437097.1"
                     /translation="MSQTITQGRLRIDANFKRFVDGEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHHTNVQSVQANIAQTEFS
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            22567..24153
                     /locus_tag="KJL99_002398"
     CDS             22567..24153
                     /locus_tag="KJL99_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate permease LctP family transporter"
                     /protein_id="HBD4437098.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGATVAFGALGVPILVAGQVTGIDPF
                     HIGAMAGRQLPFLSVLVPFWLVAMMDGWKGVKETWPAALVAGGSFAVTQFFTSNYIGP
                     ELPDITSALVSIVSLALFLKVWRPKNTETAISMGQSAGAMVVNKPSSGGPVPSEYSLG
                     QIIRAWSPFLILTVLVTIWTMKPFKALFAPGGAFYSLVINFQIPHLHQQVLKAAPIVA
                     QPTPMDAVFKFDPLSAGGTAIFIAAIISIFILGVGIKKGIGVFAEMLISLKWPILSIG
                     MVLAFAFVTNYSGMSTTLALVLAGTGVMFPFFSPFLGWLGVFLTGSDTSSNALFGSLQ
                     STTAQQINVSDTLLVAANTSGGVTGKMISPQSIAVACAATGMVGRESELFRYTVKHSL
                     IFASVITLLQAYVFTGMLVS"
BASE COUNT         5955 a         6063 c         6624 g         6226 t
ORIGIN      
        1 acctgtgttt tgattatttc taaaggtttt gaatgaatgc ttattgtctg atacacaaga
       61 aataacactc tttttatcgt taaaaaatga tatttcactt tgcccatgcc gttaaagtca
      121 ctgataatgc gtccgttcgt aaattcaaaa tggcgtaatc taatatatgc taaatttatt
      181 tgttggcctt gatatatata cggggctttt gttattactt gctctggcat ttgtgttgtt
      241 ctacgaagca atcaatggtt ttcatgacac ggcgaatgcg gtggcaaccg ttatttatac
      301 tcgtgccatg cagccacaac ttgctgtggt gatggcggca ttttttaact tttttggcgt
      361 gttattgggc ggacttagcg ttgcctatgc cattgtccat atgttaccaa tcgatttgtt
      421 gctgaatatg gggtcaactc acggtttggc gatggtcttt tccatgctgc tggcggcgat
      481 tatctggaac ctgggaacgt ggttctttgg tttaccggcc tccagttcgc acaccttgat
      541 tggtgcgatt atcggcatcg gtttaaccaa cgcgctgtta accggctcat cggtgatgga
      601 tgcgttaaac ctgcgtgaag taaccaaaat tttctcctcg ctgattgttt cccctatcgt
      661 cggcctggtc attgcgggtg gtctgatttt cctgctgcgc cgtttctgga gtgggacgaa
      721 aaagcgtgac cgtattcacc gcattccgga agatcgcaaa aagaaaaagg gcaaacgtaa
      781 accgccattc tggacgcgta ttgcgctgat tgtttccgct gcgggcgtgg cgttttcgca
      841 cggcgcgaac gacgggcaga aagggatcgg cctggtaatg ctggtactgg tggggattgc
      901 ccctgctggc ttcgtcgtca atatgaatgc gtccggctat gaaattaccc gtacccgcga
      961 tgccgttacc aacttcgaac actacctgca acagcatcct gaactgccgc agaagttgat
     1021 tgcgatggaa cctccattgc ctgcagcatc gactgatggc acgcaagtaa cagagtttca
     1081 ctgtcatccg gcaaatacct ttgatgcgat tgcgcgcgtt aaaacgatgc tgccaggcaa
     1141 tatggaaagt tacgagccgt taagcgtgag tcagcgcagc cagctgcgcc gcattatgct
     1201 gtgcatctcc gatacctccg cgaagctggc gaaactgcca ggcgtcagta aagaagacca
     1261 gaacctgctg aaaaaactgc gcagcgatat gttaagcacc attgagtacg ctccggtgtg
     1321 gatcatcatg gcggtagcac tggcgctcgg cattggcacc atgattggct ggcgtcgtgt
     1381 agcgatgacc atcggtgaga agattggtaa gcgcggcatg acgtatgcgc aaggcatggc
     1441 ggcacaaatg acggcggcag tgtctatcgg ccttgccagt tatattggga tgcctgtctc
     1501 cacaacacac gtcctctcgt ctgcggtagc agggacgatg gtggtggacg gcggtgggtt
     1561 acagcgtaaa acggtaacca gcatcctgat ggcgtgggtg tttactttac cggcggcaat
     1621 ttttctttct ggtgggctgt actggatagc attgcagttg atttaacctt cctgaaaatg
     1681 cccggtcctg gcgatcgggc atttccattt ttgactagtg ataaccacgc gcggtcataa
     1741 aatccgtaat cgctttttct gcatcaacca acacctgttc cagtggctga ttggcatcga
     1801 tatcaaccag ttgtgcacca ccaaaggtta actgtggcgt tatggcaatc ttcctcgcca
     1861 gcgattcccg tttatggtcg ggtttacgtg cacaggcaac ttcaaggtca acattgagtt
     1921 tgatgaccag atcaggctta tggctcgcca tccagtgaaa cgctttacgt tcctggcttg
     1981 ccagccatga gacaaaacga ccaccttcaa cgttaggtgg gaacaccgta ccatcgtaag
     2041 cgccaggaat ttggtcctga ggataacggt cggttagaac aattaacccg cgacgacgac
     2101 aggcaagcat atgacgaaag cgcagtaagc gacgggcgac aaacgctgta attaccagcg
     2161 ccggaactgg tccaggcaat ttttttgctg ttttcacctg atttcgttca attgttttat
     2221 gtaaggattt tcccatcaaa ggtaattttg tcactgcacg accgacattt ccggcctgtt
     2281 ttcctaaatg aactctttcg gcagcaccat atttttcgac aacggtaata agatgttcac
     2341 acaccgttga cttgcctgaa gcatcgctac caataacggc aattaatgga ggtgttattg
     2401 actgcatgaa tatatccttt aatttaaata tccattaaaa atatttattt ggttaatatg
     2461 tttttatgaa agcgtaattc aggtcaatgt cacaattagt cattgtcaca ataaggttga
     2521 acggatattc tgagcacgga ccacgtaata ttcaatacat tatttactgc cgggtttttc
     2581 gtgaatcaac gtaatatgtc aataattaat tccacgccag tgcgtgttat tgccattgta
     2641 ggatgtgatg gttcaggtaa atcgaccctc acggcaagcc tggtaaatga actggcagca
     2701 agaatgccaa cagaacacat ttatctcggg caatcgtccg ggcgaattgg cgaatggatt
     2761 tcacagctcc ctgttattgg cgcaccttgt gggtgttatc tgcgaagtaa agcggcacat
     2821 gtacacgaaa agcccttaac tccgcctggc aatattactg cgctggttat ctatctgctt
     2881 tcctgctggc gggcgtacaa gtttcgcaaa atgttgtgta aaagccagca aggctatctg
     2941 ctcatcaccg accgctaccc gcaggttgaa gtgccgggtt ttcgctttga tggcccgcaa
     3001 ttggcaaaaa ccacgggcgg taacggttgg ataaaaatgt taaggcagcg cgagttgaag
     3061 ctgtaccaat ggatggcgtc ttatttaccc gtattgttga ttcgtcttgg cattgatgaa
     3121 caaaccgcgt ttgcgcgtaa acctgaccac caactggcag cgttacagga aaaaatcgcc
     3181 gttacgccgc aactgacatt caatggcgcc aagatccttg aactggatgg tcggcaacca
     3241 gccgatgaaa ttatgcaagc gtcactacgc gcaattcacg ccgcgctttc ctgatcacca
     3301 aaatggatag gggatgattt aacgaatgga tgcactacaa acacaaactg ttaatagtac
     3361 aaccgcgccg cagcccaact acattccggg gctgattgcg gtggtcgggt gtgatggcac
     3421 cggtaaatcc acgctgacca ccgacctggt gaaatcgctg caacaacact ggcaaaccga
     3481 gcgccgctat ctggggctgc tctccggcga agacggcgac aaaatcaaac gattgccgtt
     3541 ggttggcgtc tggctggaac ggcgattggc ggccaaatcc tcgaaaaccc aaagcatgaa
     3601 aaccaaatct ccggcgctat gggcggcggt gattatgtac tgcttctcgc tgcgaagaat
     3661 ggcgaatcta cgcaaggttc agcgactggc gcaaagtggc gttctggtgg tcagcgatcg
     3721 cttcccgcag gctgaaattt cgggctttta ttatgacgga ccggggatcg gcgtcgaacg
     3781 tgcgaccggg aaaatcagca tgtttctggc gcagcgcgaa cggagtttat accaaaaaat
     3841 ggcgcaatac cgtcctgaat taatcattcg tttggggatt gatattgata ctgccatctc
     3901 ccgcaagcct gaccatgagt atgccgagct gcaggacaaa atcggcgtca tgtcgacgat
     3961 tggctataac ggcacaaaaa ttcttgagat tgattccaga gcgccctaca gcgaagtgct
     4021 ggaacaggta cagaaggcgg tttctctggt tgccatcgtt tctgaccgcc gaagtttaac
     4081 ttaaggctgg cgggatattt tttactatca acgataaggt caggctgaat tggcgggttt
     4141 taacatcaaa cattggtttg cagatggcgc gtttcgcacc attattcgca atagcgcctg
     4201 gttaggctcc agtaatgtcg agagcgcctt gctggggctg ttggcgctct cgtgtgccgg
     4261 taaagggatg acgcccgcca tgtttggtgt actggtgatt gtgcaatcgt acgccaagtc
     4321 gatcagcgat tttattaagt ttcagacatg gcagctggtg gttcagtacg gaacgccagc
     4381 cttgaccaac aataatccgc agcaatttcg caatgtcgtc tcattttcct tctcgctgga
     4441 tatcgtcagc ggcgcggtgg cgattgtcgg cggcattgcc ttactgccat tcctttccca
     4501 ttcattaggt ctggatgacc aaagcttttg gctggcggcg ctgtattgca cgctcattcc
     4561 ttcaatggct tcctccacgc cgaccggcat tctgcgtgcg gtagatcgct ttgatttgat
     4621 tgctgtacag caggcgacga aaccttttct gcgcgcagcg gggagcgtcg cagcctggta
     4681 ttttgacttt ggttttgtgg gttttgttat tgcctggtac gtgtcgaatc tggttggcgg
     4741 caccatgtac tggtggtttg ccgcgcgcga attacgccgt agaaatatcc ataacgcctt
     4801 caaattgaat ctgtttgagt ctgcccgaca cattaaaggc gcgtggagtt ttgtctggtc
     4861 aaccaacatt gcccactcca tctggtcggc gcgtaactcg tgcagcacag tgttggtggg
     4921 gatcgtgtta ggacccgctg ccgccgggtt atttaaaatc gccatgacat tcttcgacgc
     4981 cgccggaacg ccagcgggtt tgctgggtaa aagtttttac ccagaggtga tgcgtttaga
     5041 tccgcgcacc accagaccgt ggttgttagg tgtgaagtcc ggtttactgg cgggcggaat
     5101 cggtattctg gtggcgctcg cggtgttgat cgtcggcaag ccgctcattt cgctggtgtt
     5161 tggcgttaag tatctcgaag cgtatgacct gattcaggtg atgctgggcg caattgtgat
     5221 ttcgatgctg ggcttcccgc aggagtcatt gctattgatg gcgggaaaac agcgcgcttt
     5281 tctcgtggcg caaaccatcg cctcaatcgg ctacatcgta ttgctgttca tgttctgtca
     5341 tctgtttggt gtgctgggag cagcgtttgc ttacttcggt gggcaatgtc tggacgtggc
     5401 gctctcgtta attccaacct taaaggcatt ttttcagcgc cattccttgc tttataacgc
     5461 agccggagag aaatcctgat gatgagaaaa tatttcccgc ttgaggcgag cgagcgtcta
     5521 tttgttgcga ttgaagaaga tgatgtcgtt gatgcacagg ttagtttgcc gccgactatt
     5581 gcactaagtt gtaccaccga aatcattcat gataattacg cgttatgcct gcaattctgg
     5641 ctcaatggcg ttgatcgtca ggaattactg cgtttagttc gcaaacaagc caaaggcgat
     5701 gagctgactg ctgatgagcg caaacaattt aaatacatgc gagcgcgcta caagcacctg
     5761 cgttttgcgc aacggctgta tttaaagaag catcaggccg gatttttatt cggcaaaacg
     5821 accgtttttc tggggcgttt tcaggacggc tttcgtaacg gtaagaaaaa tattgtgtcg
     5881 tattacggca acctgttacg gatttattta agctctccgg tctggtcgtt ggtgaactat
     5941 tcattacgcc atagccagtt agaaagcgta agcagtttta ttgcctatcg gcaaaagcaa
     6001 atgcataccc tgaaagagat tattgccaaa ccgcgactaa ccggcaggga atttcatgat
     6061 gtgcgaaaaa tcatcagcca gcaggtttct tattacgaca ccttaagatc gcttgatcct
     6121 gaaaataaag aggcgttaca gatttcccgt ttcctcgccg ccattaatgg cctgatggga
     6181 gataaacacg acgacatggt ggcggatgat atggaaaacc gccagtcgta ttatgcaccg
     6241 ttggcgctgg atagcgatat ttgtcagcgc ttagagctgc tgatttcgcg ttttccgcta
     6301 tagccggagc aatgatgtca tctcgtttga ttattgcgtt gattatcatg ctcttagccc
     6361 cgggtgtgca ggcacacaat ttcgtcactg gcaaaacggt tacccctgtt tatatccagg
     6421 aaggcggcga gctgcttctg aatagcgacg acgaaattca ctaccaaaag tggaacagca
     6481 cacaattagc gggaaaagtc cgcattattc agtatatcgc cgggcgtaag tcggcgaaga
     6541 aaaagaactc actgttaatt aaagcggttg aagcggcaaa ctttccgcag gatcgttttc
     6601 agcccaccac cattgtgaac accgatgatg ccatctttgg tactggctat tttgttgttg
     6661 gcaaaatcga aaaaaataaa cgccgctatc cgtgggcgca attcgttatt gacggcaatg
     6721 gacagggacg cgttgcctgg cgcttgccgg agcagagttc aaccattctg gtgctgaata
     6781 aagccgggca aattcagtgg gcaaaggacg gcagcctgac gccggaagag gtagatcatg
     6841 tcattgcgtt ggcgcagaaa ctgatcaatg aataaaaaac tcaaaggatt gagggcgtta
     6901 gtgagatgaa gctggttgaa cactacatca tgcgtggtac gcgccgtctg gtgctgatta
     6961 tcgtcggttt tctgatcttt attttcgcca gctactccgc acagcgttat ctgaccgaag
     7021 cggcaaatgg gacattagct cttgatgttg tgctggatat cgttttctac aaagtactga
     7081 ttgcactgga gatgttgtta cctgttggtc tgtatgtgtc agttggcgtg acgctagggc
     7141 agatgtatac cgactcggaa attaccgcta tctctgcggc ggggggcagt ccgggacgct
     7201 tgtacaaagc cgttctttat ctggcgatac cgctaagtat ttttgtcacc cttctgtcga
     7261 tgtatggtcg tccgtgggct tatgcgcaga tttatcaact ggagcagcag tcgcagtcgg
     7321 agctggatgt tcgccagttg cgggcaaaga aatttaacac taacgataac ggacgaatga
     7381 tcctttcgca gacggttgat caggataata atcgcctgac tgacgcgctg atttatactt
     7441 ctactgccaa tcgaacccgt attttccgcg ccggttcggt tgatgtggtt gacccatcac
     7501 ccgagaaacc gaccgttatg ttgcacaacg gcactgccta tcttctcgat catcaggggc
     7561 gtgacgacaa cgaacagatc taccgtaatc tgcaattaca tctgaatccg ctggatcaaa
     7621 gcccgaacgt caaacgcaaa gcgaaatcgg tcacggagct ggcgcgctcc gcctttcctg
     7681 ccgatcatgc cgaactgcaa tggcgacaaa gccgtggcct gaccgcattg ttgatggcgc
     7741 tgctggccat ttcattaagt cgggtaaaac cgcggcaagg gcgattttca acgttattgc
     7801 cactgacgtt gctgtttatt gccatttttt atggcggcga cgtctgccgt acactggtgg
     7861 ctaacggcgc gattcccctc attcctggtt tgtggttagt gcccggactc atgctaatgg
     7921 gcctgctgat actggtcgca cgtgacttct ctttgctgca gaaattttcc cgatgaatgt
     7981 tttcagtcgc tatttaatcc gtcatctctt tctcggtttt gccgccgctg cagggctatt
     8041 gctgccgctt tttaccacct tcaacctgat taacgaactg gatggtgtca gcccgggcgg
     8101 ttatcgctgg actcaggcgg tgctggtggt gctaatgacc ttaccgcgca cactggtcga
     8161 actttcgccg tttatcgcgt tattgggtgg tattgttgga ctggggcagt tatcgaaaaa
     8221 cagtgagctt accgccattc gcagcacggg gttttctatc ttccgtattg cactggtggc
     8281 gctggttgcc gggatattgt ggactgtttc gttaggcgcg atagatgagt gggtggcgtc
     8341 gccattgcag caacaggcgt tgcaaatcaa atcgaccgcc accgcgttgg gggaggacga
     8401 tgacattacc ggcaatatgc tgtgggccag gcgtggtaat gaatttgtga cggtgaaatc
     8461 gctgaacgag cagggccagc cggtgggcgt ggagatattt cattatcgcg acgatctctc
     8521 gcttgaatcc tacatttatg cacgcagtgc caccattgaa gacgacaaaa cgtgggtcct
     8581 gcatggtgtg aatcataaaa aatggctgaa tggcaaagaa acgctggaaa cattagataa
     8641 tcttgcctgg caatcggcct tcaccagtat gaatcttgaa gagttatcaa tgccggggaa
     8701 tactttttct gtccgtcagc ttaatcatta catccattat ttgcaggaaa ccgggcaacc
     8761 cagcagcgaa taccaccttg cactgtggga aaaactgggg caacccatcc tgaccctggc
     8821 gatgattttg ctggctgtgc cgtttacctt tagtgccccg cgttcgcctg ggatgggtag
     8881 ccgtctcgct gtaggcgtca tcgtgggctt actcacctgg atcagctatc aaatcatggt
     8941 taatctggga ttgctatttg cgttaagcgc acctgttacc gcgctcggtt taccggtagc
     9001 gtttgtgttg gtggcgttga gcctggtgta ttggtatgac agacaacatt aaacccgccg
     9061 tggcgcaaac accacggcgg gctcacatta ctgcttcaga gtagcaaatg cctgaatgat
     9121 ggcatcgatt tgctcacgac tgtgggcagc atttacgcta cagcgcagca gcgtgatgcc
     9181 tgcaggcgcg gcaggcggca ggataagatt cacatacacg cccggcgaga taagctcgcg
     9241 ccagatgcgt aaaccttcct ctttcgaacc gattattact ggcactaccg ggctaatatg
     9301 tgaacttaac tcataaccca gtttttgcag accgtcatac agatggttgg cgttatccat
     9361 cagcttttgc cgcagctctg gttgtgtggc aatagttctt aacgacgagc gcacggtcgc
     9421 aatgcaggac ggagaaggag aggcggtaaa aatatacggg cggctgccgt agcgcagcac
     9481 ttccatcgct tccgaaacga cagcaaaacc gccaatcgag gccagacttt tactgaaagt
     9541 accaacgata atatccacat catcttcaac gccgacagct tcagccagtc cgcgccccgt
     9601 tgcgcctaac acgccaaacg aatgcgcttc atcgacaatc aaataaccac cgagacgacg
     9661 tttgatatca acaatttccg ccagcggcgc aacgtcaccg agcatgctgt agatgccctc
     9721 aacgatgata atcgcctctt tcgcccgttc accgaggcga accatgcgcc gttccagatc
     9781 tttagcatcg ttatggcgga aacgaataat ttccgcgcca ccgagagaac agacgtcata
     9841 aatactggcg tggctgtcgg catccagtaa caccacggcg ttatggtcgg caagtgcgct
     9901 gatgacgcct aaattagcgg tataaccggt tgaaaaacga tagcggtagg gcgattgaaa
     9961 aactcggcaa tctctttctc taacgccagg tgcggcgcat aactgccgtt tgccatacga
    10021 gaaccggtgg tgccggtgcc ttgggccgct aacgcggcct gaccttcgct gatggcatca
    10081 tgattaaagg ttaaaccgag ataattattg gtcccggcca gaatcacttt tttattgccg
    10141 atccggcctt cggtggcgga atacacttca tcaatacagg tgccaaacgg tgttaacccg
    10201 ttgtcactaa attgcagacg ctcgccagca aggcgcgcat atttatcgta tagccccatt
    10261 atttattctc cagccatgga agtagagttt ccattaattg cgcaggggtt ttgacatcca
    10321 gcagaatatt aataggaatg gaaatatcga atctgtcttc cagcatcatt aagagatcca
    10381 tcactttgat cgactccagg ccaagatcgt tgacgagatc gctgtcgggc ttaatttcca
    10441 caccgttttc gaccaaatcc tgtaaacagg aaaggatgta atccattact atttcacgat
    10501 ttaccataga aacgttaaca cacctttaaa acaagacaat caaaatagcc cgttgcgtaa
    10561 tgcgtgttcc aggctaatcc cgggaaacca attgatatct tcagaaatac ggttattact
    10621 tgccgaccag tcggcgtggg ttaattcacg aattttcgag cgggtcagca taggttcttt
    10681 acctgccagg cgactcaacg cggtactgat atccgcaagg cctgtgagta ccggcagagg
    10741 aatgtcaacc attcgcacgg aaccacaacg ggctttggcg gcaagctgtc gtacgcgttg
    10801 ccagtcatag ccaccggcga cgccatcgca taattcatag gtttgcgtct gtacagtttc
    10861 ggcgcttaac cactgaccca ctgcttgcgc aaaatcggtg acgtgaagaa aagagagttg
    10921 tgtatctggc gcaccaagtc gtggcagcag gccgcgtagc atccagtcaa acagcggttt
    10981 taactcttta tcgccgggac catacacggc tgtcgggcga aaaacgccca gcgtaatttc
    11041 gtccgccatt gctgccagtc gttgctcggc gacgtatttg gattttgcat accaggagag
    11101 ctcgggatgg cgcgccgcca gcgaagagat aaacagaaaa cgttggcaaa agccgctctc
    11161 ttttgctgct tgcatcaggc gcaggctgcc gtcaacgtta cagcgggtga aaatctcttc
    11221 tttgtgcccg cgcacttgcc cggcgcaatg gaccactacg ctggctccgg caaccagctc
    11281 gctaagtgaa tgagtatctt ccagcgaacc gcgcacccag gtgagattat cattgacgtg
    11341 agcgcgggca gtacgcgtca atgcgcgaac gtgaaagccg cgggcgagca ggttatcgat
    11401 aatatattta ccgataaacc cggtagcgcc cgtcaccgcg acagtttgat ttatgccagg
    11461 gattcctgca cattaagact ggcagcataa gccttctgat aacgtttttt cgcttccgca
    11521 cgggcaggct tgccggagga cgttcgggga atactgtggg gcggcaacag atcgatagcc
    11581 gcggtcacgc caaattcgct ttggatccgt gccgccagcg cgtggataag ctgcccgcga
    11641 cgttcttcgt cgctgatccg acactggatc tgcaaaatga ttttttcctg ggcggtaaca
    11701 aaagcaatcg catcgccaga atgaatttcc ggttcctgtt ccgctatata ttcaatatcc
    11761 tgcggccaga tattacggcc acgaataata atcagatctt taatgcgtcc ggtgacatac
    11821 agataaccgt ccagcagata accgaggtcg ccggtgtcta accagcccgt cgcggcaatc
    11881 tcgtcttgcg aaacctggtc gccaaagtaa ccgctcatca gactgggacc ggagatgcaa
    11941 atatggccta ccacacgttc cgcgaccggc atacctgctt cattgcggat ttcaatacca
    12001 tgttccggca acgctttgcc gcagttgacg aaagtcgata cggcgcgtgt ctctgcaccc
    12061 ggcgcgacgg ctttaccctg atattcgagg atgtcgcgat ccacttcgtt aaccacaacc
    12121 ccggaggctt catcagagaa gctgacagcc agcgcatttt ctgccagtcc gtagcacggc
    12181 atgaaagttt tattgtcaaa gttaacctga cggaaacatt cagcgaattg atggagttgt
    12241 tctgcggaga tcggctcagc accaatacca gcgacgcgcc agcaggaaag atccagttca
    12301 gcgagatctt tttcattcac gcggcgttgg cacaattcat agccaaacgg cggcgcaacg
    12361 gaaacggtgc cgcgattttt actgatcaat ttaagccatt gcaaaggacg catggcaaaa
    12421 tcctgagtgc gcaaataatc tactgaaagt tgcgtggcga cggggggcag gagaaagccg
    12481 accagtccca tatcatggta gaaaggctgc caggagacgc agcggtcgct agggcgtaat
    12541 ttaatgccat cgtggcttat agcacgtaga ttagccatca cttcgcgatg ggtgataatg
    12601 acgccacggg gaaaacgggt gctgccggag gtgtactgga ggtaggcgat atcgttcgga
    12661 actggacgct ggagcgcaac atcggcttcc ggtaatgcct taaaccaagc gtggcttaaa
    12721 acatgtaatt cggggttgtt atgcgtcgcg gcattgacca gtggcaacca ctcatcacca
    12781 gtgataatgg ctgcgggctg gcaacttgcc agtaaaccct ggagtttggc gctccaggaa
    12841 tcccgctgac cgacgcccat tggaatcgcc aacgggacgg cgactaagcc agcatactga
    12901 caggcaaaaa aagcctctac aaacccgcta ctcgtttcgg caatcagcgc cacgcgttcg
    12961 ccttttttca ggttcagcga taacaaccgc ttcgcaccag cttcggcacg cgtttttaat
    13021 gtctgatatt ccagttgatc ttcaagttgg caacgtctgt cataaaaatt cattccggct
    13081 ctactcagag cggcgtagtc caaagcatca accagcgttg gaaaatcggc atagcgcata
    13141 ggtagggaat gcgtaaagat tttattagac atatacacca cgaaaaaatt ggatatcaaa
    13201 aataaaatga caataatgcg gtggattata taggcataaa agataaatga gatagtattt
    13261 tcatcggtgt gaaaatgaac attgatttaa ataatgagtt taagtttttt gtttttatta
    13321 tgaatgatat ttaatttatg aatatattaa gaagaagcct tgtccggtcg ccttaaattg
    13381 acaaaaggca ttgaggcgtt tctgtgtagc ctctgacctt atcataatca ttaattaata
    13441 ctatttattc gtcgtttgta ttcattgagt tgattattaa gatgccgctc gttgctgttg
    13501 caacctgttt ttcaccatta attatttgtt atccacagag atatggaatg cgatctgctg
    13561 atgattaaaa ttgaaaaagt aattaataaa aatgacctta aggcatttat cgcttttccg
    13621 tcatcacttt atcccgacga tccaaactgg ataccccctt tattcattga acgcaacgag
    13681 catttgtctg cgaaaaatcc ggggacggac catatcatct ggcaggcgtg ggtggcaaaa
    13741 aaagcggggc agatagttgg gcgtattacc gcgcaaatag ataccttgca ccgtgagcgt
    13801 tacggtgaag ataccggtca tttcggcatg attgacgcca ttgatgatcc gcaggatttt
    13861 gccgcgttgt ttggcgcagc ggaagcgtgg ttgaagtcac aaggtgcaag taagatcagc
    13921 gggcctttca gcctgaatat caatcaggaa agcggattac tgattgaagg ttttgacaca
    13981 ccaccctgtg cgatgatgcc acacggcaaa ccgtggtatg ccgcgcatat tgaacaactg
    14041 ggttatcaca aaggcattga tttactggcg tggtggatgc agcgaaccga tctcactttc
    14101 tctccggcgc taaaaaaact gatggatcag gtgcgcaaaa aggtgaccat tcgctgcatc
    14161 aatcgtcagc ggtttgccga agagatgcag atcctgcgtg agattttcaa ctctggctgg
    14221 cagcacaact ggggatttgt gccgtttacc gaacatgaat tcgcgaccat gggcgaccaa
    14281 cttaaatatc tggtgccgga cgatatgatc tacatcgctg agattgattc tgcaccctgc
    14341 gcgtttattg tggcttgcct aacatcaacg aggcgattgc cgatctgaac ggatcgctct
    14401 ttcccttcgg ctgggcaaaa ttgctgtggc gtttgaaagt cagcggtgtg cgaactgcgc
    14461 gagtaccgct gatgggcgtg cgcgacgagt atcagttcag ccgcatcggc ccggtgattg
    14521 cgctgttatt gattgaagcc ttacgcgatc cgtttgcccg ccggaagatc gatgcgctgg
    14581 agatgtcatg gatcctcgaa accaatacag gcatgaataa catgctggaa cgcattgggg
    14641 cggagccgta caagcgttac cgattgtatg aaaagcaaat ttaataccag atcctgttta
    14701 gcgcaggggc ggtcattcgg gagagggtat agatgaacag atttaacacc agatcctgtt
    14761 aagtgcaggg gcggtcccct cgccttgggg atagggtaaa actctgctca cattcccacc
    14821 ttgttcatct ccagcggcac cgccgagcga atcaaatggt ggccttccag ctcgatatcg
    14881 tggagattct ttttcacggt ccgtacatga tcgcgcgctg cgcgctgagc gacgtgcggc
    14941 agccgctgca acaccgcgtt gtagatccgc gcatgctggc gatcgatctg ctttttttgc
    15001 tgtggtcgat ggtagagatt atttacgctg gcaaacactg aattaaacat cagatcggtc
    15061 aatgattgca gcgtaaacac cagcacctga ttgtgagaag cctgacaaat ggcgagatgg
    15121 aaagcgtgat ccaactgcgc atgttcgatc agcgaaatct ctttgtggtt ctcactggcg
    15181 gcgagcattt tttcataaca gcgggttatc acaacaaaat cagcctgcgt tcccagcgtt
    15241 gccgccagcc ttgccgattc gccctccagt aatgcgcgaa cgtcgagcag atcgtacagc
    15301 gttcgcggct gcgtactgaa cagatggatc agcgggctgg tgtcctgctc ccgattaagc
    15361 cgtgcgacat gagaatcgcg accctgcgcc gtttcaataa tcccgcgccc gcgcagcacg
    15421 gtcagccctt cacgcagtgc ggagcgtgag aagccgagct tttcacacag tcgacgttcc
    15481 gagggaagcg gctgaccgac cttcagtacg ccgtcgataa ttaaccgttc gatactctct
    15541 gcaaccactt cgcaaatagg gcgacgttca tctttcattc ccggacctcg tgcacaggta
    15601 ggaccaattt ttctgttctt aactcgcaaa acacgcacat cacgcaagtg attatgttac
    15661 atcaatttaa cattaagtta accaagacaa ggtcacagag ctggaaaaaa aatggtctga
    15721 ccggtagcta aagagataga cgaaaacgaa aagcccgctt aataactgtt cacagaagca
    15781 gcgcgcaaaa atcagctgcc acacaacaca acaaagcgaa gcctactcat gagcatcttg
    15841 tacgaagagc gtcttgatgg cgctttaccc gatgtcgacc gcacatcggt actgatggca
    15901 ctgcgtgagc atgtccctgg acttgagatc ctgcataccg atgaggagat cattccttac
    15961 gagtgtgacg ggttgagcgc gtatcgcacg cgtccattac tggttgttct gcctaagcaa
    16021 atggaacagg tgacagcgat tctggctgtc tgccatcgcc tgcgtgtacc ggtggtgacc
    16081 cgtggtgcag gcaccgggct ttctggtggc gcgctgccgc tggaaaaagg tgtgttgttg
    16141 gtgatggcgc gctttaaaga gagcctcgac attaaccccg ttggtcgccg cgcgcgcgtg
    16201 cagccgggcg tatgtaacct ggcgatctcc taggccgttg cgccgcataa tctctattac
    16261 gcgccggatc cttcctcaca aatcgcctgt tcaattggcg gcaatgtggc ggaaaatgcc
    16321 ggtggcgtcc actgcctgaa atatggtctg accgtacata acctgctgaa aattgaagtg
    16381 caaacgctgg acggcgaggc gctgacgctg ggatcggacg cgctggattc acctggtttt
    16441 gacctgctgg cgctgttcac cggatcggaa ggtatgctcg gcgtgaccac cgaagtgacg
    16501 gtaaaactgc tgccgaagcc gcccgtggcg cgggttctgt tagccagctt tgactcggta
    16561 gaaaaagccg gacttgcggt tggtgacatc atcgccaatg gcattatccc cggcgggctg
    16621 gagatgatgg ataacctgtc gatccgcgcg gcggaagatt ttattcatgc cggttatccc
    16681 gtcgacgccg aagcgatttt gttatgcgaa ctggacggcg tggagtctga cgtacaggaa
    16741 gactgcgagc gggttaacta catcttgttg aaagcgggcg cgactgacgt ccgtttggca
    16801 caggacgaag cagagcgcgt acgtttctgg gccggtcgca aaaatgcgtt cccggcggta
    16861 ggacgtatct ccccggatta ctactgcatg gatggcacca tcccgcgtcg cgccctgcct
    16921 ggcgtactgg aaggcattgc ccgtttatcg cagcaatatg atttacgtgt tgccaacgtc
    16981 tttcatgccg gagatggcaa catgcacccg ttaatccttt tcgatgccaa cgaacctggt
    17041 gaatttgccc gcgcggaaga gctgggcggg aagatcctcg aactctgcgt tgaagttggc
    17101 ggcagcatca gtggggaaca tggcatcggg cgagaaaaaa tcaatcaaat gtgcgcccag
    17161 ttcaacagcg atgaaatcac gaccttccat gcggtcaagg cggcgtttga ccccgatggt
    17221 ttgctgaacc ctgggaaaaa cattcccacg ctacaccgct gtgctgaatt tggtgccatg
    17281 catgtgcatc acggtcattt accttttcct gaactggagc gcttttgatg ctacgcgagt
    17341 gtgattacag tcaggcgctg ctggagcagg tgaatcaggc aattagcgat aaaacgccgc
    17401 tggtgattca gggcagcaat agcaaagcct ttttaggtcg cccagtcacc gggcaaacgc
    17461 tggatgtgcg ttgtcatcgc ggcattgtta attacgaccc gaccgagctg gtgataaccg
    17521 ctcgtgccgg aacgccgctg gtggcgattg aagcggcgct ggagagcgcc gggcaaatgc
    17581 ttccctgcga gccgccgcat tatggtgaag aagccacctg gggcgggatg gtcgcctgcg
    17641 ggctggcggg gccgcgtcgc ccgtggagcg gttcggtccg cgattttgtc ctcggcacgc
    17701 gcatcattac cggcactgga aaacatctgc gttttggtgg cgaagtgatg aaaaacgttg
    17761 ccggatacga tctctcacgg ttaatggccg gaagctacgg ttgtcttggc gtgctcactg
    17821 aaatctcaat gaaagtgtta ccgcgaccgc gtgcctctct gagcctgcgt cgggaaatca
    17881 gcctgcaaga agccatgagt gaaatcgccg agtggcaact ccagccatta cccattagtg
    17941 gcttatgtta cttcgacaat gcgttgtgga tccgccttga gggcggcgaa ggatcggtaa
    18001 aagcagcgcg tgaactgctg ggtggcgaag aggttgtggg tcagttctgg cagcaattgc
    18061 gtgaacaaca actgccgttc ttctcgttac caggtacctt atggcgcatt tcattaccca
    18121 gcgatgcgcc gatgatggat ttacccggcg agcaactgat cgactggggc ggggcgttac
    18181 gctggctgaa atcgacagcc gatgacaatc aaatccatcg catcgcccgc aacgctggcg
    18241 gtcatgcgac ccgctttagt gccggagatg gtggctttgc cccgctatcg gctcctttat
    18301 tccgctatca ccagcagctt aaacagcagc tcgacccttg cggcgtgttt aaccccggtc
    18361 gcatgtacgc ggaactttga ggagcaggct atgcaaaccc gattaaccga agagatgcgg
    18421 cagaacgcgc gctggaagcc gacagcatcc tgtgcgcctg tgttcactgc ggattttgta
    18481 ccgcaacctg cccaacctat cagcttctgg gcgatgaact ggacgggccg cgcgggcgca
    18541 tctatctgat taaacaggtg ctggaaggca acgaagtcac gcttaaaaca caggagcatc
    18601 tcgatcgctg cctcacttgc cgcaattgtg aaaccacctg tccttctggt gtgcgctatc
    18661 acaatttgct ggatatcggg cgtgatattg tcgagcagaa agtgaaacgc ccactgccgg
    18721 agcgaatgct gcgcgaagga ttgcgccagg tagtgccgcg tccggcggtc ttccgtgcgc
    18781 tgacgcaggt agggctggtg ctgcgaccgt ttttaccgga acaggtcaga gcaaaactgc
    18841 ctgctgaaac ggtgaaagct aaaccgcgtc cgccgctgcg ccataagcgt cgggttttaa
    18901 tgctggaagg ctgcgcccag cctacgcttt cgcccaacac caacgcggca actgcgcgag
    18961 tgctggatcg tctggggatc agcgtcatgt cagctaacga agcaggctgt tgtggcgcgg
    19021 tggactatca tcttaatgcg caggagaaag ggttggcacg ggcgcgcaat aatattgatg
    19081 cctggtggcc cgcgattgaa gcaggtgccg aggcaatttt gcaaaccgcc agcggttgcg
    19141 gcgcgttcgt caaagagtat gggcagatgc tgaaaaacga tgcgttatat gccgataaag
    19201 cgcgtcaggt cagtgaactg gcggtcgatt tagtcgaact tctgcgcgag gaaccgctgg
    19261 aaaaactggc aattcgcggc gataaaaaac tggccttcca ctgcccgtgt accctacaac
    19321 atgcgcaaaa gctgaacggc gaagtggaaa aagtgttact tcgtcttgga tttaccttaa
    19381 cggacgttcc cgacagccat ctgtgctgcg gttcagcggg aacatatgcg ttaacgcatc
    19441 ccgatctggc acgccagctg cgggataaca aaatgaatgc gctggaaagc ggcaaaccgg
    19501 aaatgatcgt caccgccaac attggttgcc agacgcatct ggcgagcgcc ggtcgtacct
    19561 ctgtgcgtca ctggattgaa attgtagaac aagcccttga aaaggaataa aaaaatgaaa
    19621 actaaagtca ttcttagcca gcaaatggcg agtgcaatta ttgccgcagg tcaggaagag
    19681 gcgcagaaaa ataactggtc tgtttccatt gctgttgccg atgacggcgg tcatctgctg
    19741 gcgttaagtc gcatggacga ttgcgcgccg attgcggctt acatctccca ggagaaagcg
    19801 cgtaccgccg cgctggggcg tcgtgaaact aagggctatg aagagatggt gaacaacgga
    19861 cgtaccgcgt tcgtgactgc gccgttactg acgtcgctgg aaggcggcgt accggttgtt
    19921 gttgatgggc aaattattgg tgccgtgggc gtttctggtt taaccggagc acaggatgcg
    19981 caggtcgcga aagtggcagc agcggtgttg gcgaaataag cgaaaacgag gagagaaaca
    20041 atgagtcaaa ccataaccca gggccgttta cgcattgacg ccaattttaa acgttttgtg
    20101 gatggagaag ttttgccggg cgtggaactg gacgctgctg cattctggca caatgttgat
    20161 gagatcgttc acgatctggc accagaaaat cgtcagttgc tggcagaacg cgatcgcatt
    20221 caggcggcgc ttgatgagtg gcatcgcagc aatccggggc cggtaaaaga taaagcggcc
    20281 tataaatctt tccttcgtga actgggctac ctggtgccgc aaccggagcg cgtgacggtg
    20341 gaaaccacgg gcattgacag cgaaatcacc agtcaggcgg ggccacagct ggtggttccg
    20401 gcaatgaacg cccgctacgc gctgaacgcg gcgaacgctc gctggggctc actgtacgat
    20461 gcgttatacg gcagcgacat catcccgcag gaaggggcga tggtcagtgg ctacgatccg
    20521 cagcgcggtg agcaggttat cgcctgggtt cgacgtttcc tcgatgaatc actaccgctg
    20581 gaaaacggca gctatcagga tgtggtggcg tttaaggtgg tcgataaaca attacgcatc
    20641 cagttgaaaa atggtaaaga aaccacgtta cgtactccag cacagtttgt cggttaccgt
    20701 ggcgatgccg ctgcgccgac ctgcattttg ctgaaaaata acggcctgca tattgaactg
    20761 caaattgatg ccaacgggcg gattggcaaa gacgatccgg cgcacatcaa cgatgttatc
    20821 gtcgaagctg ctatcagtac cattctcgac tgcgaagatt cggtcgcggc ggttgatgcg
    20881 gaagataaaa tcctgctgta ccgcaatctg ctgggcctga tgcaggggac tctgcaagag
    20941 aaaatggaga aaaacggtcg gcaaatcgta cgtaaactga atgacgatcg tcagtacacc
    21001 gccgccgatg gctctgaaat ttctctgcac ggacgctcgt tgctgtttat ccgcaacgtg
    21061 ggtcatttga tgaccattcc tgtgatttgg gacagcgaag gcaatgaaat cccggaaggt
    21121 attcttgatg gcgtcatgac tggcgcgatt gccctctatg atttaaaagt gcagaaaaac
    21181 tcgcgcactg gcagcgtcta tattgtgaaa ccgaaaatgc acggtccgca ggaagtggcg
    21241 ttcgccaaca aactgtttac ccgcattgag acaatgctcg gtatggcacc gaataccctg
    21301 aaaatgggca ttatggatga agaacgccgg acctcgctga acttgcgtag ctgtatcgct
    21361 caggcgcgca accgcgtggc gttcatcaat accggtttcc tcgaccgtac cggcgatgaa
    21421 atgcattcgg tgatggaagc tggcccgatg ctgcgtaaaa accagatgaa atcgacgccg
    21481 tggatcaaag cctacgaacg taataacgtg ctttccggtc tgttctgtgg gctgcgcggt
    21541 aaagcgcaaa tcggtaaagg catgtgggca atgccggacc tgatggcaga catgtacagc
    21601 cagaagggcg accaactgcg tgccggggca aacacagcct gggttccgtc accaaccgct
    21661 gctacgctcc atgcgctgca ctaccaccat accaacgtac agagcgtgca agccaacatt
    21721 gcccagaccg agttcagtgc tgaatttgaa ccgctgctgg acgatctgct gactattccg
    21781 gtggctgaaa acgctaactg gtcggcgcaa gagatccaac aagagctgga taacaacgtg
    21841 caggggattc tggggtacgt ggtgcgttgg gtggagcagg ggattggttg ttcaaaagtg
    21901 ccggatattc acaacgtggc gctgatggaa gaccgcgcaa cgctgcgtat ctccagccag
    21961 catatcgcca actggttacg tcacggtatt ctgaccaaag aacaggtgca ggcgtcgctg
    22021 gagaatatgg cgaaagtggt tgatcagcaa aatgctggcg atccggctta tcgtccgatg
    22081 gcggggaatt tcgctaactc gtgtgctttt aaagctgcca gcgatttaat cttcctcggc
    22141 gtgaaacagc caaacggtta taccgaaccg ttattacacg cctggcgttt acgcgaaaaa
    22201 gaaagtcatt aatagcgtca aaaaagcccc ggcaagtttt attgtcgggg ctatgatagt
    22261 aactgcggta gtacatttat cccggaaata accggagcga ttatcagggt ttaacaatat
    22321 taaatacccg tagcgcatta ctagcgggcc taaaacaaca atatggacat cttaacgata
    22381 ctctgcgcct gcgtgtgcgg aacagaaacc cttacccgat atacttgtta tgccagaggt
    22441 agcaattgcg ttagcggcgt actgttgctg taaagtataa cgcctatatt ttacagttca
    22501 gtcctctttt gcttattaca accttaagta tatcaagcat ataaagataa taagagactg
    22561 aacaatatgg ttacctggac ccaaatgtat atgccgatgg gaggactggg gctatccgct
    22621 ctggtcgccc tgatcccgat aatattcttc ttcgttgcac tcgcggtatt acgtctgaaa
    22681 ggacatgttg ctggagcaat aacccttata ttatctatcc tgattgcgat attcgccttt
    22741 aaaatgccga ttgatatggc atttgctgct gcgggctatg gctttattta tggattatgg
    22801 ccaattgcgt ggattattgt cgcggcggtg ttcctgtata aattaaccgt tgccagcggg
    22861 cagttcgata ttatccgcag ctcggttatc tccatcaccg acgatcagcg tttgcaggtg
    22921 ttactgattg gtttctcctt tggtgcgttg ctggaaggag ctgctggctt tggtgcgacg
    22981 gtggcgtttg gtgcgttggg cgtgccgatt ctggtcgccg gtcaggtaac gggaatcgat
    23041 ccgttccaca ttggcgcaat ggcgggacgt cagttaccgt tcctgtcggt tcttgtgccg
    23101 ttctggctgg tagcaatgat ggacggctgg aaaggggtga aagagacgtg gccagcggcg
    23161 ctggttgctg ggggaagctt cgctgtcact cagttcttta cctctaacta tattggtccg
    23221 gaactgccgg atattacttc ggcgctggtg agtatcgtct cactcgcttt attccttaaa
    23281 gtctggcggc cgaaaaatac cgaaacggca atcagcatgg ggcaatccgc aggtgcgatg
    23341 gtggtaaata agccatcttc tggcggtccc gtgccttcag aatatagtct ggggcaaatc
    23401 attcgtgcgt ggtcaccgtt tttaatctta acggtgctgg tcaccatctg gaccatgaag
    23461 ccgtttaaag cgttatttgc tccgggcggc gcgttttatt cactggtgat taatttccag
    23521 atccctcatt tgcatcaaca agtgttgaaa gcggcaccca ttgtcgccca accaacgcca
    23581 atggatgcgg tgtttaaatt cgaccctctc tcggctggcg gcaccgctat ttttattgcg
    23641 gcgattatct ctatcttcat cctcggcgtg ggcatcaaga aagggattgg cgtctttgcc
    23701 gaaatgctaa ttagtctgaa gtggccgata ttgtcgattg gcatggtgct ggcattcgcc
    23761 ttcgtcacca actattccgg catgtccacc acgctggcgc tggtactggc aggtacaggc
    23821 gtgatgttcc cgtttttctc accgtttctc ggctggctgg gcgtattcct taccgggtcg
    23881 gacacttctt caaacgccct gtttggttca ctgcaatcga ccacggcgca gcaaatcaac
    23941 gtctctgaca ctctgctggt ggcagcaaat accagcggcg gcgtaactgg caagatgatc
    24001 tccccgcaat ctatcgccgt ggcctgcgcc gcgacgggca tggtgggccg agaatctgaa
    24061 ctgttccgct acaccgtgaa gcacagtctg atttttgcca gcgttatcac cctgctgcag
    24121 gcgtatgtgt ttaccgggat gttagtctcg taaatcaata aagccggatg aaggtaaata
    24181 tcacccggct ttccatttgt ctgtcgttta tctcgccagg gaaccgtttt tacgattggc
    24241 ttctttatac gtagtctccg ttttacgatg cgaattgttt aatatttata caacttggct
    24301 tctgtgctgg ttataaagta cccactttca tgagatcaat tttagtatgt tattacattg
    24361 cttcatctgt tgctttcatg acgaatggcg ttttttctct tatttatatt tttcactaga
    24421 acaatgagta gatcaaaatt cccaaaactc ccaaaaacat aagattattc gcaaagctga
    24481 ttttgcagaa taatgagaac ttttaagatt tgtcgaggat caattttaag caatttatct
    24541 ccacttgtta cataaaagat gccgttaggt taataagtta atcttgctat aacagtgttc
    24601 gttactgcct attaataaag atatttggga tagggtaacg tggatgagga ttcgaaatca
    24661 tccgtcttgc ggaaagtgaa atgttggcag atgttaatat tatgtatgta ttttaactcc
    24721 aacaacacac caatcatttt gttagaaata agaaaattta tggttaaata atttggtgat
    24781 tatgaagcat taaatcgtaa tgccaaatat tgttcgcttt ggagagtatt attgcctgtt
    24841 tgtgtctgtt gagcagaaaa atcatctg
//
LOCUS       DAEBXN010000058        24837 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.058, whole genome shotgun sequence.
ACCESSION   DAEBXN010000058 DAEBXN010000000
VERSION     DAEBXN010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24837
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.058"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(24..100)
                     /locus_tag="KJL99_002399"
     tRNA            complement(24..100)
                     /locus_tag="KJL99_002399"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(64..66),aa:Arg,seq:acg)
     gene            complement(165..241)
                     /locus_tag="KJL99_002400"
     tRNA            complement(165..241)
                     /locus_tag="KJL99_002400"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(205..207),aa:Arg,seq:acg)
     gene            complement(245..337)
                     /locus_tag="KJL99_002401"
     tRNA            complement(245..337)
                     /locus_tag="KJL99_002401"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(301..303),aa:Ser,seq:gct)
     gene            complement(653..838)
                     /gene="csrA"
                     /locus_tag="KJL99_002402"
     CDS             complement(653..838)
                     /gene="csrA"
                     /locus_tag="KJL99_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HBD4437099.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(1073..3703)
                     /gene="alaS"
                     /locus_tag="KJL99_002403"
     CDS             complement(1073..3703)
                     /gene="alaS"
                     /locus_tag="KJL99_002403"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HBD4437100.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADIDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMNLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3831..4331)
                     /gene="recX"
                     /locus_tag="KJL99_002404"
     CDS             complement(3831..4331)
                     /gene="recX"
                     /locus_tag="KJL99_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HBD4437101.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4574..5635)
                     /gene="recA"
                     /locus_tag="KJL99_002405"
     CDS             complement(4574..5635)
                     /gene="recA"
                     /locus_tag="KJL99_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HBD4437102.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5715..6212)
                     /gene="pncC"
                     /locus_tag="KJL99_002406"
     CDS             complement(5715..6212)
                     /gene="pncC"
                     /locus_tag="KJL99_002406"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HBD4437103.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6357..7442)
                     /gene="mltB"
                     /locus_tag="KJL99_002407"
     CDS             complement(6357..7442)
                     /gene="mltB"
                     /locus_tag="KJL99_002407"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HBD4437104.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAIMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7698..8260
                     /gene="srlA"
                     /locus_tag="KJL99_002408"
                     /gene_synonym="gutA"
                     /pseudo
     CDS             7698..8260
                     /gene="srlA"
                     /locus_tag="KJL99_002408"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
     gene            8257..9216
                     /gene="srlE"
                     /locus_tag="KJL99_002409"
                     /gene_synonym="gutE"
     CDS             8257..9216
                     /gene="srlE"
                     /locus_tag="KJL99_002409"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBD4437105.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            9227..9598
                     /gene="srlB"
                     /locus_tag="KJL99_002410"
                     /gene_synonym="gutB"
     CDS             9227..9598
                     /gene="srlB"
                     /locus_tag="KJL99_002410"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBD4437106.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9602..10381
                     /gene="srlD"
                     /locus_tag="KJL99_002411"
     CDS             9602..10381
                     /gene="srlD"
                     /locus_tag="KJL99_002411"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HBD4437107.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSRMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10486..10845
                     /gene="gutM"
                     /locus_tag="KJL99_002412"
     CDS             10486..10845
                     /gene="gutM"
                     /locus_tag="KJL99_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HBD4437108.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVDR
                     SSGRFKPRVVVAIALDDQQCIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10912..11685
                     /gene="srlR"
                     /locus_tag="KJL99_002413"
                     /gene_synonym="gutR"
     CDS             10912..11685
                     /gene="srlR"
                     /locus_tag="KJL99_002413"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HBD4437109.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQIVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11678..12643
                     /gene="gutQ"
                     /locus_tag="KJL99_002414"
     CDS             11678..12643
                     /gene="gutQ"
                     /locus_tag="KJL99_002414"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HBD4437110.1"
                     /translation="MSEALLNTGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEVLMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12640..14154)
                     /gene="norR"
                     /locus_tag="KJL99_002415"
     CDS             complement(12640..14154)
                     /gene="norR"
                     /locus_tag="KJL99_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HBD4437111.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14341..15780
                     /gene="norV"
                     /locus_tag="KJL99_002416"
     CDS             14341..15780
                     /gene="norV"
                     /locus_tag="KJL99_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HBD4437112.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAMDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            15777..16910
                     /gene="norW"
                     /locus_tag="KJL99_002417"
     CDS             15777..16910
                     /gene="norW"
                     /locus_tag="KJL99_002417"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HBD4437113.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV"
     gene            complement(17038..19290)
                     /gene="hypF"
                     /locus_tag="KJL99_002418"
     CDS             complement(17038..19290)
                     /gene="hypF"
                     /locus_tag="KJL99_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HBD4437114.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYIV
                     HDVHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQALVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19443..19970)
                     /gene="hydN"
                     /locus_tag="KJL99_002419"
     CDS             complement(19443..19970)
                     /gene="hydN"
                     /locus_tag="KJL99_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HBD4437115.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVPVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(20119..21129)
                     /gene="ascG"
                     /locus_tag="KJL99_002420"
     CDS             complement(20119..21129)
                     /gene="ascG"
                     /locus_tag="KJL99_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HBD4437116.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESS
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            complement(21143..21262)
                     /locus_tag="KJL99_002421"
     CDS             complement(21143..21262)
                     /locus_tag="KJL99_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur protein"
                     /protein_id="HBD4437117.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAQGQSANGAVHCVDCV"
     gene            complement(21272..22981)
                     /gene="hycE"
                     /locus_tag="KJL99_002422"
     CDS             complement(21272..22981)
                     /gene="hycE"
                     /locus_tag="KJL99_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HBD4437118.1"
                     /translation="MSEEKLGQHYLAALNEAFLGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHPHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGLARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSYAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(22999..23922)
                     /gene="hycD"
                     /locus_tag="KJL99_002423"
     CDS             complement(22999..23922)
                     /gene="hycD"
                     /locus_tag="KJL99_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HBD4437119.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWMFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(23925..>24837)
                     /locus_tag="KJL99_002424"
     CDS             complement(23925..>24837)
                     /locus_tag="KJL99_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="catalyzes the oxidation of formate to carbon
                     dioxide and molecular hydrogen; formate hydrogenlyase
                     comprises of a formate dehydrogenase, unidentified
                     electron carriers and a hydrogenase (subunit 3); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HBD4437120.1"
                     /translation="GVTGIALEQPALIALGLVGGLYHLLNHSLFKSVLFLGAGSVWFR
                     TGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEWVIYQSFFKLSNSGAF
                     VARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAENATCAPLLMSVSVVAL
                     AICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLIACPLLPFIIMAICKG
                     DRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKLRKWLNPVSLVPGWQC
                     EGSALLFRRMALVELAVLVVIIVSRGA"
BASE COUNT         5862 a         6983 c         6370 g         5622 t
ORIGIN      
        1 ctaaagccat atcaagtaag agatggtgca tccgggagga ttcgaacctc cgaccgctcg
       61 gttcgtagcc gagtactcta tccagctgag ctacggatgc atcgggaaac ttactttact
      121 gcagattttt tgataccgct actaaagccg tatcaagtaa gagatggtgc atccgggagg
      181 attcgaacct ccgaccgctc ggttcgtagc cgagtactct atccagctga gctacggatg
      241 caaatggcgg tgaggcgggg attcgaaccc cggatgcagc ttttgaccgc atactccctt
      301 agcaggggag cgccttcagc ctctcggcca cctcaccaca cgcctcttac gagtgcttcg
      361 aagaacttgt ttatcgctca tcatcgctgc gtggcgcaca tattactttc tgagacttat
      421 aagtcaaaca atttttccca cacttttatc gtttgcacac ttcacgctca attagtctgt
      481 aaaagcgtca aaaagagtgt tttatctaca gaaaaacgga ggtctggcag atagcagtaa
      541 tgcaaaaaaa tggagactta agttgaatga acgggagtaa agcgaaaaga ctatagagtg
      601 aaggagaaat tttgagggtg cgtctcaccg ataaagatga gacgcggaaa gattagtaac
      661 tggactgctg ggatttttca gcctggatac gctggtagat ctcttcacgg tgaacagaaa
      721 cttccttcgg ggcatttacg ccaatacgta cctggttgcc ctttacccct aaaactgtca
      781 cggtgacctc atccccaatc atgagggtct caccaactcg acgagtcaga atcagcattc
      841 tttgctcctt gaaagattaa aagagtcggg tctctctgta tcccggcatt atccatcata
      901 taacgccaaa aagtaagcaa tgacaaacac attacatgta agcagtcatg gcattacatt
      961 ctgttaaacc taagtttagc cgatatacac aacttcaacc tgactttatc gttgtcgata
     1021 gcgttaatgc gaatgccgtg aagcgagtcc acggcattgc ctgacgctta tattattgca
     1081 atttcgcgct gacccagcct ttcacactgg ctaacgctgc aggtaaggcc gcagcatccg
     1141 taccaccggc ttgcgccatg tcaggacgtc caccaccctt gccgcccacc tgctgagcga
     1201 ccataccaat cagttcccct gctttcacac gatctgtgac gtccttagat acgcctgcaa
     1261 tcagagaaac cttaccttcg gctaccgttg ccagcacgat aattgtcgac cccagctgat
     1321 tttttaaatc gtcaaccatg gtacgcaaca ttttcggctc aacaccgcta agctcgctaa
     1381 ccaacagctt aacaccatta acatcaattg ccttactgga aagatttgcg ctctcctgtg
     1441 cggcagcttg ttctttaagc tgttgtagtt ctttttctaa ctgacgcgta cgttccagta
     1501 ctgagcgcac tttatccgcc agattattgc tatcgccttt cagcagatgc gcgacttcgc
     1561 ttaagcgatc gctgtctgca tgaacggtgg tgatagcgcc ttctccggtt accgcttcga
     1621 tacgacgaac gcctgcagca gtacccgatt cagagatgat gcggaacaga ccaatatcac
     1681 cagtgcggct ggcgtgagta ccgccacaca gctcggtgga gaaatcgccc atgctcagca
     1741 cgcgtacacg ctcatcatac ttctcgccga acagcgccat cgcacctttc gctttcgccg
     1801 cttcgagatt catgatgttg gtttcgatcg gcaaattgcg acgaatctgt gcgttcacca
     1861 ggtcttcgac cgcacgaatc tcttccggtt tcatcgcttc gttgtgtgag aagtcgaagc
     1921 gcagcacctt gtcgttaacc agtgaacctt tctgcgatac atgagtcccc agaacctggc
     1981 gcagcgcagc gtgcatcagg tgcgttgcgg agtgattcag acgaatacgg gcgcgacgag
     2041 cctcatcaat atcagcctgc acagcgtcgc ccactttcag agaacccgca gcaagtttac
     2101 cgatgtgacc aatcgcctgg ccgtatttct gcgtatcttc caccgcgaag gagaagttag
     2161 cgcctttcag ttcgccttta tcaccaacct gaccgccgga ttccgcatag aatggcgttt
     2221 gatccagcac gaccacagct tcctggccgg cattgatggc atcaaccgct ttaccatcaa
     2281 caaacagcgc ggtcactttg ccgttcagtt ccagatggtc atagccttta aattcagatg
     2341 cactatcaac acggatcatt gcgttgtaat cggcaccaaa gccgctggct tcgcgcgcgc
     2401 gacgacgctg ctcttccatt gctgcttcaa aaccagcttc gtcaactttg atgttgcgct
     2461 cacgacaaac atcagccgtc aggtcaaccg ggaagccata ggtgtcgtac agacggaaag
     2521 ccgtttcacc atccagcgta tcaccagaaa gttttgccag ctcttcatcc agcaacgcca
     2581 gaccgcgctc cagagtacgg gcaaactgct cttcttcagt cttcagcacc tgctcaacct
     2641 gcgcctgctg gcgtttcagg tcttcacccg cagagcccat aacgtcgatc agcggaccaa
     2701 ccagtttgta gaagaaggtt tctttcgcgc cgagcatatt gccgtgacgc actgcgcgac
     2761 gaatgatacg acgcagtaca taaccacggt tttcattgga cggcattacg ccatccgcga
     2821 tcaggaacgc acaagaacga atgtggtcag cgattacgcg cagcgattta ttgctcagat
     2881 cggtcgcgcc agtgactttc gctaccgcct ggatcaacgt gcggaacagg tcgatgtcat
     2941 agttagagtt aacgtgttgc agcaccgcag caatacgctc cagacccata ccggtatcta
     3001 cagacggctt cggcagcggt tccatcgtgc catctgcctg gcggttgaac tgcatgaaga
     3061 cgatgttcca gatctcaatg tagcggtcgc cgtcttcttc cggacttccc ggagggccac
     3121 cccaaatgtg gtcgccgtga tcgtagaaga tttcggtgca cgggccgcac ggaccagtgt
     3181 cacccatctg ccagaagttg tcagatgcgt atggcgcacc tttgttatcg ccgatgcgaa
     3241 taatacgttc gcgcgggatc ccgacttctt tttcccagat ttcgtaggct tcgtcgtcgc
     3301 tttcatagac ggtaacccac agacgctctt tcggcagggc aaaccatttt tcgctggtca
     3361 gcagttccca tgcaaactga atggcatcgt gtttgaaata gtcgccgaag ctgaagttgc
     3421 ccagcatttc gaagaaggta tggtgacgcg cggtgtaacc gacgttttcc aggtcgttgt
     3481 gtttaccacc cgcacgcacg cagcgttggg aagtggtagc gcgggaataa ttacgcttgt
     3541 cgagcccaag gaacacatcc ttgaactggt tcatcccggc gttggtaaac aacaaagttg
     3601 ggtcgttatg gggtaccagg gagctgctgg caactacctg atgtccctta ctatggaaaa
     3661 agtcgagaaa cgcctgacgg atctcagcgg tgctcttgct cataattatc ctgaaatcaa
     3721 gctaacgaaa tatcgccacc agctccagcg tgtcttaacc gccgggctgg taactgaaaa
     3781 gtgggaataa gataagtttt cttgactggg aagtaaaata ccgtatgcgt tcagtcggca
     3841 aaatttcgcc aaatatcctg gatatcttcc atcagatagc cacgatagag cagaaaacgc
     3901 tggatcttaa ctttttctga aaagacagtt ggcaaaggtt caccatattt tcgcgtcgcc
     3961 tgatcgcgcg ccagtgcgca ccagtcgatg tcacattcac gcatcgcttt ttctgtcgct
     4021 tcgcgggaaa tacctttctg attcagttcc tgacgaatac gtgcaggtcc ataccctttg
     4081 cggctacggc tggcgataaa gcgcgcaaca aatcggctgt catcgagata gccatgttca
     4141 tggcaccagg caataacgcg ctcgtaatct tctgccgtag catcaatctc ttctgggcca
     4201 tttttgccca taatcggtgc cgcgagttta cgtcgcagtt cttgctcact gtgatcgcgc
     4261 accgccagaa tgcgtaccgc acgatccaac aggcgagcat atgccgggcg acgggatgtt
     4321 gattctgtca tggcatatcc ttacaactta aaaaagcaaa agggccgcag atgcgcccca
     4381 ggttttgctg ataaacagat ttaatagtca atgagtatgc agaatgttcc cgcaggccca
     4441 attcgttaga ggatcgaggt gagagtaatt tttcacgcca gtgctggcct gttcccctca
     4501 ccctaaccct ctccccaaag gggcgagggg accgtccgtg cacacattat ctattatcat
     4561 caataacgaa cgattaaaaa tcttcgttag tttctgctac tccttcgctg tcatctacag
     4621 agaaatccgg cgttgagttc gggttgctca gcagcaactc acgtactttc ttctcgatct
     4681 ctttcgcggt ttccgggtta tctttcagcc aggcagttgc attcgcttta ccctgaccga
     4741 tcttctcacc tttgtagctg taccacgcgc ctgctttctc gatcagcttc tcttttacgc
     4801 ccaggtcaac cagttcgccg tagaagttga taccttcgcc gtagaggatc tgaaattcag
     4861 cctgtttaaa cggtgcagcg attttgttct tcaccacttt cacgcgggtt tcgctaccca
     4921 ccacgttttc gccctctttc accgcgccga tacgacggat gtcgagacga acagaggcgt
     4981 agaatttcag cgcgttacca ccggtagtgg tttccgggtt accgaacatc acaccaattt
     5041 tcatacggat ctggttgatg aagatcagca gcgtgttgga ctgcttcagg ttacccgcca
     5101 gcttacgcat cgcctggctc atcatacgtg ccgcaaggcc catgtgagag tcgccgattt
     5161 cgccttcgat ttccgctttc ggcgtcagtg ccgccacgga gtcaacgacg ataacgtcta
     5221 ctgcgccaga acgcgccagg gcgtcacaga tttccagtgc ctgctcgccg gtatccggct
     5281 gggagcacag caggttatcg atatcgacgc ccagtttacg tgcgtagatt gggtccagcg
     5341 cgtgttcagc atcgataaac gcacaggttt taccttcacg ctgcgctgcg gcgatcacct
     5401 gcagcgtcag cgtggtttta ccggaagatt ccggtccgta gatttcgacg atacggccca
     5461 tcggcagacc acctgcccca agcgcgatat ccagtgaaag cgaaccggta gagatggttt
     5521 ccacatccat ggaacggtct tcacccaggc gcatgatgga gcctttacca aattgtttct
     5581 caatctggcc cagtgctgcc gccaacgctt tctgtttgtt ttcgtcgata gccattttta
     5641 ctcctgtcat gccgggtaat accggatagt caatatgttc tgttgaaaca attatactgt
     5701 atgctcatac agtatcaagt gttttgtaga aattgttgcc acaaggtctg caatgcatac
     5761 gcagtagcct gacgacgcac cgcatcacgg tcgccgctga agcattcccg ccgggtaatg
     5821 ccttcaccgc gggcagtggc aaaagcaaac cagacggtgc cgacaggctt ctcttcactg
     5881 ccgccatccg gcccggcgat accactaata gacacggcat aatcagcacg agccgctttc
     5941 agtgcaccta tcgccatttc caccacgacg ggttcactca ccgcgccatg ctgcgccagc
     6001 gtctcttcgc gtacgccgat catctgcgct ttggcttcgt tactgtaggt gacaaatccg
     6061 cgttcaaacc aggcggagct accggcaata tcggtaatca ctttcgctac ccaaccaccg
     6121 gtacaagact cggcagttgt tacggttgcg ccacgggctt tcagcgcctg cccaacctgt
     6181 tcgcttaact gcatcagttc actgtcagtc atcacaacct ctgtcagtca aaaatttatg
     6241 ccggacaaga tagcactttc gtcaggaaga tggtgttggc gttgcaattt tacgaggagg
     6301 attcagaaaa aagctgatta gccagaggga agctcacgcc cccctcttgt aaatagctac
     6361 tgtactcgcg ccagcgccac ggcttgtcct aactgccaga ccgccattgc gtaatgggtg
     6421 ctgtggttgt aacgggtgat ggtgtagaag ttcggcagac cgtaccagta ctggtagccg
     6481 gtgccaacat ccagacgcag caggctggct tgttgatggt tgcccagcgg ctgctgtggc
     6541 gttaaacctg cggcggcaag ctgcgaaatg ctgtacttag ttttgaaacc atttggcaag
     6601 cctggagcct gaccgtttgc cattatcgcg acctgatcgc ctttcaccca gccgtgcgct
     6661 ttgaaatagt tcgccacgct accgatcgca tcaaccggat cccacaggtt gatatgcccg
     6721 tcgccgctga aatctaccgc atattgtttg taagacgacg gcataaactg tccgtagccc
     6781 atcgccccgg caaaggaacc tttcagattg agcggatcgt cctgctcgtc gcgcgccatc
     6841 agcaggaagg tttccagctc gccagagaaa tactccgcgc ggcgtgggta gttaaatgac
     6901 agcgttgcca gcgcatcgag gatgcgagtt ttccccatca cgcgccccca gcgggtttca
     6961 acgccgataa tcccgacgat aatttccggc ggtacgccat acacctgcca cgcgcgattc
     7021 aacgcatctt catactgatt ccagaaaacc acaccgttct gcacgttgtc cggcgtaata
     7081 aattttttgc gataacgcag ccatgcgccg ttcggacctg atggcggttt caccgatgtg
     7141 gttggtgcct ggttatccat cagccgcagt accgaatcca gacgcttcgc ctgggagaga
     7201 atttcctgca actgctgacg atcgaaaccg tgtttgttca ccattttgtc gatgaactgc
     7261 tgggcattcg ggttattagc gaaatcgccg cccatctgca tcacattgtg ctgcggctca
     7321 agcaggaagc cgccagacgg cgttccggtg gtcgtttcag tctcagtagg ttttggcttg
     7381 ctgctacagg cggcaagcaa cacaaaaagg ggaagcaatg ttacataacg acgcttgaac
     7441 atgaggggtc catttaacag attcaaccag gggcaagtat ggtaaagcat cacaccccgc
     7501 acaaggaagc ggtagtcact gcccgatacg gactttacat aactcaactc attcccctcg
     7561 ctatactttt attcaaactt tcaaattaaa atatttatct ttcattttac gatcaaaata
     7621 acacttttaa acctttcaac ctgattagat taggttgccg tttggtaata aaacaataaa
     7681 tcctgaagga gagaacaatg atagaaacca ttactcatgg tgcagagtgg tttatcgggc
     7741 tgttccaaaa gggcggagag gtgtttaccg ggatggtgac cggcattctt ccgctgttga
     7801 ttagcctgca ggttatcatg aacgcactga ttaattttat cggtcagcat cgtattgaac
     7861 gttttgctca acgttgcgcc ggtaaccctg tttcccgtta cctactgtta ccgtgcattg
     7921 gcacgtttgt cttttgcaat ccgatgacct taagcctggg tcgctttatg ccggaaaagt
     7981 acaaacccag ctactacgcg gcggcctctt atagctgcca ctcaatgaat ggcctcttcc
     8041 cccatatcaa ccctgcgaac tgtttgttta tcttggcatt gccagcggtc tgacaacgct
     8101 gaacctgcca cttggcccac tggcggtgag ttatctgctg gttggtctgg tcaccaattt
     8161 cttccgcggc tgggtgaccg atctgaccac cgccattttt gagaaaaaga tgggcattca
     8221 acttgaacaa aaagttcacc tggcaggagc aacatcatga cgcgtattcg gatcgaaaaa
     8281 ggaacgggtg gctggggcgg cccgcttgag ctggaagcca cgccgggcaa aaaaatcgtc
     8341 tatatcaccg ccggtacccg gcctgcgatt gttgacaaac tggcacaact tactggctgg
     8401 caggctattg acggatttaa agaaggtgaa cccgcggagg cggaaattgg tgtcgcggta
     8461 atcgactgtg gcggcacatt acgctgcggc atctatccga aacgacgtat tcccaccatt
     8521 aatatccact cgacgggcaa gtccggtccg ctggcgcagt acattgtgga agatatttat
     8581 gtctctggcg taaaagaaga aaacatcact gtagtgggtg atgcgacacc acaaccctct
     8641 tccgtgggcc gtgactatga caccagtaag aaaatcaccg aacaaagcga tggtttactg
     8701 gcgaaggtgg gaatgggcat ggggtccgcc gttgccgtgt tgtttcagtc tggtcgtgac
     8761 accatcgaca ctgtattaaa aaccattctg ccgtttatgg cattcgtctc ggcgctcatt
     8821 ggcatcatta tggcttctgg ccttggtgac tggattgccc acggtcttgc tccgctagcg
     8881 agccatccac tgggtctggt catgctggcg ctcatctgct ccttcccact gctttcacct
     8941 ttcctcggcc caggcgcagt tatcgcacag gttatcggcg tattgattgg cgtgcagatt
     9001 ggtctcggca atattccgcc gcatctggct ttaccggcac tgtttgccat caacgcgcag
     9061 gcggcttgcg acttcatccc ggtcggtttg tcgctggcgg aagcccgtca ggacacggtt
     9121 cgcgtcggtg tcccttctgt actggtgagc cgctttttaa ccggcgcgcc gactgtactg
     9181 atcgcctggt ttgtctccgg ttttatctat caatagaggc tgaaacatga ccgttattta
     9241 tcagaccacc atcacccgta tcggcgcgag tgccactgac gccctcagcg accagatgct
     9301 catcaccttt cgtgaaggcg cgccagcgga cctcgaagag tattgcttca ttcattgcca
     9361 cggcgagttg aaaggtgcac tccatcccgg tttgcaattt tcactcgggc agcatcgcta
     9421 tccggtgacc gctgttggca gcgtggcgga agacaacctt cgcgaactgg gtcatgtcac
     9481 cctgcgcttc gatggtttaa acgaagcgga atttccgggc actgtccatg tggcaggccc
     9541 tgtacccgac gatatcgcgc cgggatcggt tttgaagttt gaatctgtta aggagtaaaa
     9601 aatgaatcag gttgccgttg tcatcggtgg tgggcaaacc ttaggcgcgt tcctgtgcca
     9661 cggtctggct gccgaggggt atcgcgtcgc ggttgtcgat attcagagcg acaaagccgc
     9721 aaatgtggca caagaaatta acgccgaata tggtgaaggt atggcgtacg gttttggtgc
     9781 tgacgccact agcgagcaaa gcgttctggc gctctctcgt ggggtagatg aaatctttgg
     9841 tcgcgtggat ttgctggtct atagcgccgg aatagcaaaa gcagccttta tcagcgactt
     9901 ccagctcggc gattttgacc gttcgctaca agtgaatctg gtgggttatt tcctgtgtgc
     9961 ccgtgaattt tcgcgtttga tgatccgcga cgggattcag ggtcgcatta ttcagatcaa
    10021 ctcgaaatcc ggcaaagtgg gcagcaaaca caactctggc tacagcgcag cgaaatttgg
    10081 tggcgtcggg ctgactcaat cactggcgct ggatctggcg gagtacggca ttacggtgca
    10141 ttcactgatg ctcggtaacc tgctgaaatc gcggatgttc cagtcactgt tgccacaata
    10201 cgcgaccaag ctgggtatca aaccggatca agtcgagcag tattacatcg acaaagtacc
    10261 gctcaaacgc ggctgcgatt atcaagatgt gctgaatatg ctgctgttct acgccagtcc
    10321 taaggcgtcg tactgcaccg gacagtcgat caatgtcacc ggcggtcagg tgatgttctg
    10381 atcaacagcg gagatccgtt aaggatctcc gtgagactat agaatgcctg atgcgctacg
    10441 ctcatcaggc atacaggact tccgccacta cattaaggaa aagttatggt atccgcactc
    10501 atcaccgtcg ccgttatcgc ctggtgtgcg caactggcct taggcggctg gcaaatttct
    10561 cgttttaacc gtgccttcga cacactatgc cagcaagggc gggttggcgt ggaccgttcc
    10621 agcgggcgct ttaaaccgcg ggtcgtggtc gccatcgcgc tggacgatca gcagtgcatc
    10681 gtcgacacct tgtttatgaa aggactgact gtcttcgccc gaccgcaaaa aattcccgca
    10741 attaccggca tgcatgcggg tgatttacag cccgatgtga tctttcccca tgatccacta
    10801 tcacagaatg ctctatcatt ggcgcttaaa ctgaaacgtg gataatttcg ttgtgaatgt
    10861 tacttgcttg cgaagttatc attttgaaac ctaaatcagg taatcacgcc catgaaacct
    10921 cgtcagcgtc aggccgccat tctggagtat ctgcaaaagc agggtaaatg ctcggttgaa
    10981 gaattggcgc aatactttga caccacaggc acaaccattc gcaaagatct ggtcattctg
    11041 gaacatgccg gaaccgtcat tcgtacttat ggcggagtgg tgttgaataa agaggaatcc
    11101 gatccgccta tcgatcataa aacgctcatc aacacccaca agaaagagct gattgcagaa
    11161 gctgccgtta gttttatcca tgatggcgat tcgatcattc ttgatgctgg cagtaccgtt
    11221 ttgcagattg ttcccctgct ctcgcgcttt aataacatca cggtgatgac caacagcctg
    11281 catatcgtca atgcgctatc cgaactggat aacgaacaaa ctatcctgat gccaggcgga
    11341 acatttcgca aaaaatcggc ctcatttcac gggcaactgg cagagaatgc cttcgagcat
    11401 ttcaccttcg ataaattgtt tatgggcacc gacggcatcg atctcaatgc gggcgtaacc
    11461 acgttcaacg aggtttatac cgtcagtaag gcaatgtgca atgccgcgcg cgaagtgatt
    11521 ttgatggcgg actcatcaaa gtttggccgt aaaagcccca acgtagtttg cagtcttgaa
    11581 agcgtcgata agctaattac cgacgcaggt atcgatccgg cgtttcgtca ggcgctggaa
    11641 gagaaaggga tcgatgtgat cataaccgga gagagcaatg agtgaagcac tactgaacac
    11701 gggacgtcag acgttaatgc tggagttgca ggaagcaagc cgtttaccgg aacgtctggg
    11761 cgatgatttt gttcgcgccg ccaatatcat cctgcactgc gaaggcaaag tggtggtttc
    11821 gggaattggc aaatcgggcc acattggtaa gaaaatcgcc gcaacacttg ccagtaccgg
    11881 cacaccggct ttttttgtcc atccggcaga agcactgcac ggcgatctgg ggatgattga
    11941 aagccgcgat gtgatgctgt ttatctctta ctccggtggc gcgaaggaac tggatctgat
    12001 tattccgcgt ctggaagata aatctatcgc gctgctggcg atgaccggca aaccgacgtc
    12061 accgctgggc ctggcggcga aagcggtgct ggatatctcc gtagaacgcg aagcctgccc
    12121 gatgcacctt gcgccgacct ccagcaccgt caataccctg atgatgggtg acgcgctggc
    12181 gatggcggtc atgcaggcgc gcggttttaa tgaagaagat tttgcccgct cccacccagc
    12241 tggggcactg ggcgctcgct tgctgaataa agtgcatcat ctgatgcgtc gtgatgacgc
    12301 tatcccacag gtagcgttaa ccgccagcgt gatggatgcg atgctggaac tcagccgcac
    12361 cggtctgggg ctggtggcgg tatgtgacgc tcaacaacag gtacaaggcg tctttaccga
    12421 cggcgattta cgtcgctggc tggtaggcgg cggcgcactc accacgccag ttaatgaagc
    12481 gatgacgaca ggcggcacca cattacaggc acaaagtcgc gccatcgacg ccaaagaagt
    12541 tctgatgaaa cgcaaaatca ctgccgcacc agtggtagat gaaaacggca aactcaccgg
    12601 cgcaattaac ctgcaggatt tctatcaggc cgggattatt taatccttca atcccagacg
    12661 tttcgccagc cgatgcaggt tggcgacgtc agtttccagc attcgcgcac aggctgccca
    12721 gttgtgatga ttttgtgcca gtgcctggcg aatagtttca cgctggaacg cttctgtcgc
    12781 ttcacgcagg ttttgcttaa caacgggcac cgccgccgct tctggcggcg gcaacgtcac
    12841 ctcaggaaaa gcaaaatgtt gcgcctcaag aatcacttca tcgccgttgc gggtggctct
    12901 cgccagcact accgcccgat gaatagcatg ttccagttcg cgcacgtttc ccggaaaact
    12961 gtagtgttgc agtaaatttc gcgccccggc acttaatacc acgcgggaga gcccctgccg
    13021 caaacgacac tgctcgcaga aataccccgc cagcagaatg acatcatcgc cccgctcacg
    13081 cagcggcggc accgaaagtg gaaacacgct caggcgatga aacaaatcgg cgcggaatcg
    13141 ccctgccagc acctcttcgc gtaagtcgcg gttagtcgcc gccagcacgc gcacatcgac
    13201 ccgcaaacta cggtcatcgc caacgcgctg aatatcgcca tactgcaaca ccctcagcag
    13261 cttggcctgc aatgccaacg acaactcgcc gatctcatcg agaaacagag taccgttatc
    13321 cgccatttca aacttcccgc tgcggttact gatagcgcca gtaaacgctc ctttcacatg
    13381 cccgaacaac tcactttccg ccacactttc cggcagtgcg gcacagttga gatagaccag
    13441 cggattcacc gcccgtggcg aggcttcatg aatcgctttc gccaccagct ccttaccggt
    13501 tcccgtctca ccgctgatca gaacgttgag atcggacgcc gccacaatct caatctcttt
    13561 tttcagttgc gtcatgccag gggacaagcc aatcatctgc gtctgtttca ccgcttcaaa
    13621 cggcgtggca tcgcctggca gcatattctg gctttccagt tgttcaatca gcaacgcatt
    13681 gcttaacgct cccgccgcca gcgcggcaat cagccgtaac tcttcgtcgc tgaaaacatc
    13741 gaactgatcg ggctgcatcc cgtcgagcgt cagtgcgccg atcaggtttt gtccgacaaa
    13801 caatggcaga ccaacgcagg cgtgaacctt cagactctcc tgcccaggaa tcaaaccgtc
    13861 atagggatcg ggcaattcgc tgtctgcggg aaagcgcacc acatccccgg cgcgggcaat
    13921 cgcttccagc cgtggatgcc cttccagcgc aaagcgtcta ccgagtacat cctttgccag
    13981 accgtcgatg gcaagcggaa taaactgccg cgaatcgtaa cgtagcaacg cagacgcatc
    14041 gcactccagc acctgacgta gcgtggtgat caggcgctga aaacgatcct gatgaccaat
    14101 cccacgctgc aattcgatgg cgatattcgc cagcacatca acggaaaaac tcatctttgc
    14161 ctcactgtca atttgactat agatattgtc atatcgacca tttgattgat agtcattttg
    14221 actactcatt aatgagcata attttattta tagagtaaaa acaatcagat aagaaactgg
    14281 cacgcaatct gcaattagca agacatcttt ttagaacacg ctgaataaat tgaggttgct
    14341 atgtctattg tggtgaaaaa taacattcat tgggtgggtc aacgtgactg ggaagtgcgt
    14401 gattttcacg gcacggaata taaaacgctg cgcggcagca gctacaacag ctacctcatc
    14461 cgcgaagaaa aaaacgtgct gatcgacacc gtcgaccata aattcagccg cgaatttgtg
    14521 cagaacctgc gtaatgaaat cgatctggcg gatatcgatt acatcgtgat taaccatgca
    14581 gaagaggacc acgccggggc gctgaccgaa ctgatggcgc aaattcccga tacgccgatc
    14641 tactgtactg ccaacgctat cgactcgata aatggtcatc accatcatcc ggagtggaat
    14701 tttaatgtgg tgaaaactgg cgacacgctg gatatcggca acggcaaaca gctcattttt
    14761 gtcgaaacac caatgctgca ctggccggac agcatgatga cttacctgac aggcgacgcg
    14821 gtgctgttca gtaacgatgc tttcggtcag cactactgcg acgagcatct gttcaacgat
    14881 gaagtggatc agacggagct tttcgagcag tgccagcgtt actacgccaa tatcctgacg
    14941 ccgttcagcc gcctggtaac gccgaaaatt accgagatcc tgggctttaa cttaccagtc
    15001 gatatgatag cgacctccca cggcgtggtc tggcgcgata acccgacgca aattgtcgag
    15061 ctgtacctga agtgggccgc tgattatcag gaagacagaa tcaccatttt ctacgacacc
    15121 atgtcgaata acacccgcat gatggctgac gctatcgccc agggaattgc ggaaaccgac
    15181 ccacgcgtgg cggtgaaaat tttcaacgtc gcccgaagcg ataaaaacga aatcctgacc
    15241 aatgtcttcc gctcaaaagg cgtgctggtc ggcacttcga cgatgaataa cgtgatgatg
    15301 ccgaaaatcg ccgggctggt ggaggagatg accggattac gcttccgtaa caaacgcgcc
    15361 agtgctttcg gatctcacgg ctggagcggc ggtgcgatgg atcgcctttc cacgcgcctg
    15421 caggatgcgg gtttcgaaat gtcgctcagc ctgaaagcca aatggcgacc agaccaggac
    15481 gctctggagt tatgccgtga acacggtcgc gaaatcgccc gtcagtgggc gctcgcgccg
    15541 ctgccgcaga gcacggtgaa tacggtagtt aaagaagaaa cctctgccac gacgacggct
    15601 gacctcggcc cacgaatgca gtgcagcgtc tgccagtgga tttacgatcc ggcaaaaggc
    15661 gagccaatgc aggacgttgc gccaggaacg ccgtggagtg aagtcccgga taacttcctc
    15721 tgcccggaat gctccctcgg caaagacgtc tttgacgaac tagcatcgga ggcaaaatga
    15781 gcaacggcat tgtgatcatc ggttcgggct tcgccgcccg ccaactggtg aaaaatattc
    15841 gcaaacagga cgccactatt ccattaaccc tgattgccgc cgacagcata gatgagtaca
    15901 acaaacccga cctcagccat gttatcagtc aggggcaacg tgccgacgac cttacccgcc
    15961 agacggcggg tgaatttgcc gggcagttta atctgcgcct gtttccgcac acctgggtga
    16021 cggatatcga tgccgaagcc catgtggtga aaagccagaa taatcagtgg caatacgaca
    16081 agttagtgct ggcaaccggt gccagcgcct ttgtcccgcc tgtgcctggg cgtgagttaa
    16141 tgctgacgtt aaatagtcag caagagtatc gcgcctgtga aacgcaactg cgggatgccc
    16201 gacgcgtgtt gattgtcggc ggtggtttga ttggtagcga actggcgatg gatttttgtc
    16261 gtgcaggcaa agcggtcacg ctaatcgaca acgctgccag tattctggcg tcattaatgc
    16321 caccagaagt aagcagccgc ttgcagcatc ggttgacgga gatgggcgtt catctgctgt
    16381 tgaaatctca gttgcagggg ctggaaaaaa cggattctgg cattctggca acgctggacc
    16441 accagcgtag tatcgaagtg gatgcggtaa ttgccgccac cggactgcgc ccggaaacag
    16501 ccctggcacg acgcgccggg ctgacgatta atcgcggcgt ttgcgtcgat agttatctgc
    16561 aaaccagtaa tgccgatatt tacgcgctgg gcgattgcgc ggaaattaac ggtcaggtat
    16621 tgccgttcct gcagccgatt caacttagcg ggatggtgct ggcaaaaaat cttctcggca
    16681 ataacacgcc gctgaaacta ccggcgatgc tggtgaaaat caaaacgccg gaattaccgc
    16741 tgcatctggc aggcgaaacc cagcgtcagg atttacgctg gcagattaat accgaacgcc
    16801 agggaatggt ggcgcgcggc gttgacgatg ctgaccagct tcgcgccttt gtggtcagtg
    16861 aggatcggat gaaagaggca tttgtattgt tgaaaacgtt gccggtgtag gtgggctact
    16921 gtgcctaaaa tgtcggatgc gacgctggcg cgtcttatcc gacctacggg gacgcatgtg
    16981 taggccggat aaggcattta cgccgcatcc ggcaagggtg tccaaatgca acacgcttta
    17041 tccgttctgg acttcacccg ctaaccaacg cgccgcagca ataaccccct gccccagaga
    17101 caaaccgcca tcacccgccg gtaaactctg tggaaagagc aatgtgaaat cagcgagata
    17161 atgcgccaga cgtgcacgca gcaaacggtt atgaataacc ccgccgctaa ataccagcgt
    17221 agtgatacca cgcatcgtgg cctgctcacg catcaacgcg gcaaaaccct gcgccagcgc
    17281 atcatgaaac gcccacgcgc gttgattaac cagtgcctgc cagttcagcc actgctgcca
    17341 gaaagtggcg agatccagtt gattgtccac ccgcggcatc gtcaccggat gcgtcactcc
    17401 gtcgcatgag gctgcgagcg cctccagagc acaagccgct tcaccttcat aacttaacgt
    17461 ggctggcgca cagcccagtg ccgccgccac tgcatcgaac aaccgcccac acgatgatgc
    17521 cagcggcgcg ttaattccac gctcaatggc ccgcgccagt acgctccagt tttgctgttg
    17581 cacacttgcc gtttcagggt aattctgcca ctccggcaca aagcgcaggc actgcgccag
    17641 caggtttcgc cacggctgct tcgccgccaa atcgccaccc ggaagcgcca ctgcaggcaa
    17701 gccgcccagg tgctcacatt cgcgatagtt cacccgcagg cactcgccgc cccacaaagc
    17761 gccattctcc cccataccga taccgtcgag cgtcaaagca atgacatcac cgccatccag
    17821 cggccactga tgctctgcca gacacgccgc tgcatgggca tgatgatgca gtaccgtttg
    17881 cgtcggcaga ttcatttcgc tcgcccactg gctggagaca tagcccggat gcacatcatg
    17941 cacaatgtat tgcggagtga aatcgtagat gttttgcatc aggcgtaacg cttcgcgcca
    18001 ctgcatctgg atgccatcgt cacttaaatc gcccagatgc tgactcaaca ccgcttgttc
    18061 accgcgcacc aggcagaagg tatttttcag atccgcgcca agacacagca caggcggaac
    18121 atttttaaag cccggaggca aaaccagcgc atccggcaca tacccccgcg aacggcgcag
    18181 catttcgccg ctttcgcgca ccactgaatc atccatccgc tgcacgatgt cgcggttatg
    18241 tagcaagaat ccgtcggcaa tgccctgcaa atccgccagc gcctgttcgt tgctgatagc
    18301 tggtggttta ccgctcaggt tgccggaggt catcaccagc gggcattgca gttcctgtaa
    18361 cagcaaatgc tggagcgggt tcgcaggcaa catcaccccg acttcgttaa ggccaggggc
    18421 gatatcatca caaagctcag gtacgtattt tttatccacc agcacaatcg gcgcagcggg
    18481 cgtggtaagc aactggcgcg cagcgtctgg taaactttct gccactggca acatgactgc
    18541 cagcggtttc gccgggcgat gtttgcgctc ccgcagtgtc gccaccgcgt tactgttacg
    18601 tgcatcgcag gcaagatgaa atccgccaat ccctttgatg gcgacaatgt tgcccatttt
    18661 taactgtgcg atagctgcct gtaatgccgc ctcttgttct gcatgttcac catgacttac
    18721 ccattcaaga tgcgggccac actccgggca ggccaccggc tgggcgtgga agcgacgatc
    18781 gagcgggtcg cggtactctt tgtcacaggc cggacatagc ggaaacgccg ccatcacggt
    18841 aaacgggcgg tcgtaaggca tcgcgcgaat aatggtgaaa cgcgggccgc agtgggtaca
    18901 gttgataaac ggataacgat aacgccgctc gcctggggta ttcatttcgg caaggcaagc
    18961 agggcaagtg gcggcatccg ggacaatttg cgtattcatg gtgccgccgg tgctctggcg
    19021 gatggtgaac tcggtgggca gttgtgacca gataaacggc tcacgctcga cgctatcaat
    19081 acgcgccagt ggcggacagt gctgatgcaa ttgaacaaga aacgtttccg ggtcttccag
    19141 cagccggact tctacgccat cgccgtcatt acatacatcg ccgtgaagat ttaattgctg
    19201 tgccagctgc cagacaaacg gacgaaaacc gacgccctgc actttgccac gaatacgcag
    19261 ttggacaccg caagatgtgt tttttgccat tgagttattc ccgccatcat gaattgcgta
    19321 acccgccctg ccggacacga cagcgtcgta tccggcagtc tcaggtcggc gataccgccc
    19381 gctccgtatt ctacgaatat ttccgggaat tcctttgatg ccagaacagt tctgtaagat
    19441 ttttagaaca tcagcgccgt acggcggcgt ttttctgcgc tcagttgttc aagtttatta
    19501 cgatcgacac aaatcagcgc atgggtcggg caagccgcca tacacgccgg gccgtcttca
    19561 cgatggttgc acaggtcgca tttattggct tcggctttgt cagcccgtac attcagaccc
    19621 gcgccgctgt tgcgaatcac aggacgtacc accacttcca tcgcgccata cgggcaagcc
    19681 accacgcagg ttttgcaacc aatgcaacgt tcctgcatca caggaacaaa ccctttatca
    19741 cggctgatag caccattcgg gcagacgtta gcgcacggtg catcttcaca ctgacggcaa
    19801 actgtcgccg tggaaatgtt cacaccttta atgacatgga tacgcggtaa aaatgtttcc
    19861 ggggtcagcg atgcacagtc ctgattttcc tgatgagaaa ccacgcacgc cacttcacag
    19921 gtacggcaac caatacattt actcgcgtca gcaatgatga aacggttcat caaattctcc
    19981 agcaatgaca gttaatgcgc cgatacactc acaaatcatg ccagttttta atttgctgtt
    20041 atttaaggaa attaatttct gtaatgcagg aaaaaagatg tcatcgacac tagtgacgat
    20101 gacatgtgat gacaatgttt atcgcgaaag ggcaatgagt gagtcgcggc ggatcagttt
    20161 tccgctaaag gttttcggcg gtgagaaatc cccgccatcg agcataaaaa tcagccgtcc
    20221 aataatttcc tgaatcatct cagtcaccgg aatttttacg ctggagagcg ccggaacgat
    20281 atagggggca attgcgatat catcgaatcc gataactgac acctgctctg gcaccgctac
    20341 gccgcgctcg tgcaacgctt tgatcgcacc tatcgccata tcgtcgttac tggcaactaa
    20401 cgcgctaaat ttagcccctc gttcgagcaa cgtttctacc ccttcggccc cgctggcggg
    20461 cgtccattta ccgttagcga taagtttttc attgagcgca ataccatgct gcgacagcgc
    20521 gtctttatac ccggcaagac gttcaatgct ggtgggggaa tccatcgagc cggtaaggaa
    20581 agcaatctcc tgatgcccgg cgtttatcaa ctctgccacg gcgttaaagc tggtctgttt
    20641 atgatcgcac cagacgctat ggctgctatt attgcgcagg cggcgattaa gcaccattat
    20701 cggctgactg tgcgcgtcaa tgatgtcatc aatctcatcc acgcttaaaa aacgcgggta
    20761 aatcataatg gcgtcgcagc gcaaatccag cagatactga atcgcctggc gctcttcttc
    20821 ggcgctgtgt ttaccatctg ccaatagcaa ctgtcgccct ttctcttccg ccattcgcgc
    20881 ggcatgaaac agtaattcac taaaataaat gccgtgataa agcgtgttgg tcactaccag
    20941 ccccagcgtc tgagtactct tcgccgacag attgcgggcc agcaaatttg ggcggtaact
    21001 gctctcttct accgcctgaa acacgcgatc tttagtctcc tggctgacgt agccattacc
    21061 tgaaagcacg cgggaaacgg tcgcttttga aaccccggcg cgcttcgcca cttccagcat
    21121 cgtcgtcatc attttcatcc ctttacacgc aatcaacgca gtgtactgca ccgtttgccg
    21181 attgtccttg cgcaatcggc tccagcggat aagacgaggt cgccgtgccg gttttgatga
    21241 cttttttgat aaaggtaaac atggcgattc cttatttcag cggcgagttt ttacgctcaa
    21301 tgctgtaacg ctcgagttct ttgtacggca ccactttgct cttcttctta cgcacatcga
    21361 ctacggtcat gcggtcggta caggagtagc aagggtccag gctaccgata atcagcggcg
    21421 cataggaaac ggtattgcca cgcagcatgt agcgcagggt cggccagttc gcgtaggtcg
    21481 cggcacggca gcgccagcgg tacagcttct ggttgtcgcc ggtcatgctc cagtggatat
    21541 cgtcgccgcg cggcgcttcg gcaaagccca gcgcgaaacg gtgcggaatg taggtaaagc
    21601 cttccaccat cagcggaccg cccggcaggt tatccagacc gtagtcgatc atattcagcg
    21661 cggtatagac ctcgttgatt cgcactttca gacgggaaat aacgtcgcag ccctgctcgc
    21721 tgtggacttc cattggcagc aggccataac cgacaaacgg gtgatcggcg cgagtatcgc
    21781 gggcgagacc gctggcgcga accatcgggc caacgttact gaaatcgcgg gcgatttccg
    21841 ggtccagacg accaatgccg acggtgcgtt gttccatgtt cggcgtactc agcagcacat
    21901 ccaccagctc ctgcacttca cgacgcattt gttgcgccag ctggcgggtc tggatcatgt
    21961 catctttcag cagatcgcga cgaatcccgc cgatcaggtt caggccgtag gttttacgcg
    22021 caccggtaag gatctctgcc attttcatgg aggtttcacg cacgcggaag aactgcataa
    22081 agccggagtc gaagccggta aagtgacagg cgaggccgag gttgagcaga tgcgggtgca
    22141 ggcgttccac ctccagcaga atggcgcgga tcatctgcgc gcgttctggc acctgaatac
    22201 ccatcgcgtt ttccaccgac gtggtgtagg cggtgctgtg ggcaaagccg cagatcccgc
    22261 acacacggtc agagaggaag gtcacttcgt tataacccat gcgggtttcc gccagttttt
    22321 ccataccgcg atggacgtag aacagacggt agtcggcgtc gataatgttt tcgccatcga
    22381 cgaacagacg gaagtgaccc ggttcgtcag aagtgacgtg cagcggacca atcggcacga
    22441 cgttgttttt cttgtcgccc agttcgttga tgaactcgta ggtttcagca tcggtagtcg
    22501 gtgccggacg ctgacgataa tccatgctgt ctttacgcag cggataaagt tcatccggcc
    22561 agtcatccgg cagcaccaga cgacgttcat ccggcagacc aaccggaatc aaaccgtaca
    22621 tatcgcgcac ttcgcgctcg ccccacaccg ccgccggaac gcgcggcgtc acggacggat
    22681 actccggttt gttggcgtca acttcgacgc gaaccgttac ccaacacttg gtgcccttct
    22741 ccatcgacag cacgtaataa acggcgtaat gaccattcag tttgcgttcg tcgttaccaa
    22801 acagcaccga cagccagcca ccctgcttgt agtagagaaa ctccaccact tccggcagat
    22861 agttcacctt tacggtgaca gtcagctgat ctttggtctg ccaggcgtgg tccagcacga
    22921 cgcctagaaa tgcctcattc agcgcggcga gataatgttg acctaatttt tcttcagaca
    22981 tgctcaaact ctctttaatc acgccgccag caaggagacg aacgctaaaa atgcaaagcc
    23041 aaacccagcc caggtaatgc gcggagtaat atcaagacgc agacgggcca tgctgttttc
    23101 gaacagcgcg ataaccagga cgccgaccac cagtttgacg atggcaatca ccagcgccag
    23161 cagcagtcca ccgacggtga aggtttccat ttgtccccac ggaataaaca ccccgacgaa
    23221 catctgcaac accaccagct gtttcaggct gataccccat ttcatgacgc caaagccgct
    23281 accgctgtat tcggagagcg gaccttcctg caactcctgc tcggcttccg ccaggtcgaa
    23341 cggcagtttg cccatttcga taaaagtggc gaacgcacag gcgcagagcg ccagcaccag
    23401 cgggatgctt tggctcagcg gccagtgata aacggtgtcg gtaatgttgc tgatgttggt
    23461 ggaaccggca acctgtgcgg caacccacag accaagcagc aacatcggtt cgaccagcac
    23521 gccaagcatc gcttcacggc tcgcaccgat agcggtaaac gggctaccgg tatccagacc
    23581 ggaaatggca aagaagaaac gcgcgatggc gaagagatac agtaaggtga tcaaatcgcc
    23641 cagttgcggc agcggagaac cgacggtcac caccggcagc gcagtagcga tagtcagcat
    23701 gacgcccacc atcacatacg gcgtcaggcg gaacatccag ccagaggcat ccgggccaac
    23761 gctctggcgg cccagcagtt tgataatgtc gcgatactcc tgcaacacgc ccggcccgcg
    23821 acggttatgc agacgggcgc gcgccacgcg ggtaataccg gagagcagcg gcgcaacggc
    23881 aaataacacc agcgcctgaa ttaacggata taaaacactc attctcaggc tcctcgtgaa
    23941 acaataatca ccaccagtac cgccagttca accagcgcca tccggcggaa cagcaacgca
    24001 ctaccttcgc actgccagcc cggcactagc gacaccggat tcagccattt gcgcagtttc
    24061 agcactggcg caaacgcctg tttaaccggc atggcaaaac cgtgagcggt aatcaccatt
    24121 gatttttcat ggtcgtagcc gcacacccag gccgcaccgc gggaacgtga cggcaggcgg
    24181 tcgcctttgc aaatcgccat aatgatgaat ggcagcagcg ggcaggcaat cagcagcaat
    24241 gtgatcattg gttgagaaac ggtggtgtta gcaggctcca gaggcagagg tacagcagca
    24301 gagagcatcg gcagcagcca cggcgcagca acaccgccaa ttacgcagca aatcgccagt
    24361 gccactacgc ttacgctcat caggagcggc gcacaggtgg cgttttcggc ttctttggta
    24421 cgcggcgcgc cgaggaacgt gacgccatag actttcgcca tacacatcac cgccagcgca
    24481 ccggtaattg ccagacccac agcgagcagc ggccccagca gacgggcaac aaacgcgccg
    24541 ctattgctca gtttgaagaa ggattgatag ataacccatt ccccggcaaa cccgttcagt
    24601 ggcggcagcg cagccattgc catcaacccg actaacatgg cgatggagat aaccggcatt
    24661 ttcttgccaa taccaccgag tttttcgata tcgcgatgac cggtacggaa ccagacgctc
    24721 cccgccccga ggaacagtac gcttttgaac aggctatggt taagcagatg gtacagacca
    24781 ccgaccaggc caagagcaat cagcgccggt tgttcgagcg cgatacccgt tacgcca
//
LOCUS       DAEBXN010000059        24064 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.059, whole genome shotgun sequence.
ACCESSION   DAEBXN010000059 DAEBXN010000000
VERSION     DAEBXN010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24064
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.059"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(25..597)
                     /locus_tag="KJL99_002425"
     CDS             complement(25..597)
                     /locus_tag="KJL99_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HBD4437121.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            815..1675
                     /gene="tesB"
                     /locus_tag="KJL99_002426"
     CDS             815..1675
                     /gene="tesB"
                     /locus_tag="KJL99_002426"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HBD4437122.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHCIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(1724..3010)
                     /gene="amtB"
                     /locus_tag="KJL99_002427"
     CDS             complement(1724..3010)
                     /gene="amtB"
                     /locus_tag="KJL99_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HBD4437123.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSVGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(3040..3378)
                     /gene="glnK"
                     /locus_tag="KJL99_002428"
     CDS             complement(3040..3378)
                     /gene="glnK"
                     /locus_tag="KJL99_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HBD4437124.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(3559..5340)
                     /locus_tag="KJL99_002429"
     CDS             complement(3559..5340)
                     /locus_tag="KJL99_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HBD4437125.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGAVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIHLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDCIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIIDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(5333..7105)
                     /locus_tag="KJL99_002430"
     CDS             complement(5333..7105)
                     /locus_tag="KJL99_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HBD4437126.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLSLMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            complement(7135..7593)
                     /gene="decR"
                     /locus_tag="KJL99_002431"
     CDS             complement(7135..7593)
                     /gene="decR"
                     /locus_tag="KJL99_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HBD4437127.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGVWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            complement(7747..8565)
                     /gene="cof"
                     /locus_tag="KJL99_002432"
     CDS             complement(7747..8565)
                     /gene="cof"
                     /locus_tag="KJL99_002432"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HBD4437128.1"
                     /translation="MARLVAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKESPALLQVFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVRTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            8665..10356
                     /locus_tag="KJL99_002433"
                     /pseudo
     CDS             8665..10356
                     /locus_tag="KJL99_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
     gene            10421..11116
                     /gene="queC"
                     /locus_tag="KJL99_002434"
     CDS             10421..11116
                     /gene="queC"
                     /locus_tag="KJL99_002434"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HBD4437129.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(11168..11566)
                     /gene="fadM"
                     /locus_tag="KJL99_002435"
     CDS             complement(11168..11566)
                     /gene="fadM"
                     /locus_tag="KJL99_002435"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HBD4437130.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGVENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILNQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(11660..12031)
                     /locus_tag="KJL99_002436"
     CDS             complement(11660..12031)
                     /locus_tag="KJL99_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HBD4437131.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(12182..14053)
                     /gene="ppiD"
                     /locus_tag="KJL99_002437"
     CDS             complement(12182..14053)
                     /gene="ppiD"
                     /locus_tag="KJL99_002437"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBD4437132.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLVAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGASG
                     INSDIITVDGDRAFVLRVSEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(14245..14517)
                     /gene="hupB"
                     /locus_tag="KJL99_002438"
     CDS             complement(14245..14517)
                     /gene="hupB"
                     /locus_tag="KJL99_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HBD4437133.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(14726..17080)
                     /gene="lon"
                     /locus_tag="KJL99_002439"
     CDS             complement(14726..17080)
                     /gene="lon"
                     /locus_tag="KJL99_002439"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HBD4437134.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDVPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVIGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(17268..18542)
                     /gene="clpX"
                     /locus_tag="KJL99_002440"
     CDS             complement(17268..18542)
                     /gene="clpX"
                     /locus_tag="KJL99_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HBD4437135.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(18668..19291)
                     /gene="clpP"
                     /locus_tag="KJL99_002441"
     CDS             complement(18668..19291)
                     /gene="clpP"
                     /locus_tag="KJL99_002441"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBD4437136.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(19538..20836)
                     /gene="tig"
                     /locus_tag="KJL99_002442"
     CDS             complement(19538..20836)
                     /gene="tig"
                     /locus_tag="KJL99_002442"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HBD4437137.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(21180..21497)
                     /gene="bolA"
                     /locus_tag="KJL99_002443"
     CDS             complement(21180..21497)
                     /gene="bolA"
                     /locus_tag="KJL99_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HBD4437138.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            21802..22380
                     /locus_tag="KJL99_002444"
     CDS             21802..22380
                     /locus_tag="KJL99_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBD4437139.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRALRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            22424..23899
                     /gene="ampG"
                     /locus_tag="KJL99_002445"
     CDS             22424..23899
                     /gene="ampG"
                     /locus_tag="KJL99_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HBD4437140.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
BASE COUNT         5637 a         6638 c         5816 g         5973 t
ORIGIN      
        1 ccgggcggag agggttaacg tcgcttaata ttgcgtagga gcgggaactg ccgaagacgg
       61 attcacctga gttggtgaag tcgaaggtac ggtagtcgtt gcgccaccgc tggcctgaac
      121 cggcacggcg gtttgctgta ccggcaccaa tgtcaggtcg gctttagttc cgccctggtt
      181 gatcaccggc tgaacggtat cggtgataaa taccagtttg tcattcacgg taatcgccgc
      241 actcaacaga atacgcgcgt tcggctgaac atctgccggg ttaaatggca gaacaaagct
      301 gaatggtgac tgtttacctt cagtacgcac cgctttctgc gccagcactt ttgacggtgc
      361 atcggctaac gacgcgtcag aaagtgtcac ggtcagcaca gcatcaggcg gcagtgcgac
      421 tttctgacgg atccagacag taccggagac attcggttgc tggatagcag gttgttgtgt
      481 tgctgaaata gacgtatttg cagccggggc tggcgtctga atatccgcgc ttttatctgc
      541 gcaagccgcc aacgcaatcg caaccgctaa accactggcc atgtgcacga gtttcattca
      601 tttctcctta ttatcaatgc accagcgggc taactttcct cgccggaaga gtggttaaca
      661 aaatagtaac gtcaacaagt gtggcacaca tcacgcattt ctgcctgtaa ttagcccgta
      721 attcagacta tttcacccat cggaccagtg gcaaatttgc gttatactca actcactttg
      781 gcttgctgcg gcagctttgt tactggagag ttatatgagt caggcgctaa aaaatttact
      841 gacattgtta aatctggaaa aaattgagga aggactcttt cgcggccaga gtgaagattt
      901 aggtttacgc caggtgtttg gcggccaggt cgtgggtcag gccttgtatg ctgcaaaaga
      961 gaccgtcccg gaagagcggc tggtacattc gtttcacagc tactttcttc gcccaggcga
     1021 tagtaagaaa ccgattattt atgatgtcga aacgctgcgt gacggtaaca gcttcagcgc
     1081 ccgccgggtt gctgctattc aaaacggcaa accgattttt tatatgacgg cctctttcca
     1141 ggcaccagaa gcgggtttcg aacatcaaaa aacaatgccg tccgcgccag cgcctgatgg
     1201 cctgccttcg gaaacgcaaa tcgcccaatc gctggcgcac ctgctgccgc cagtgcttaa
     1261 agataaattc atctgcgatc gtccgctgga agtccgtccg gtggagtttc ataacccact
     1321 gaaaggtcac gtcgcagaac cacatcgtca ggtgtggatc cgcgcaaatg gtagcgtgcc
     1381 ggatgacctg cgcgttcatc agtatctgct cggttacgct tctgatctta acttcctgcc
     1441 ggtagctcta cagccgcact gcatcggttt tctcgaaccg gggattcaga ttgccaccat
     1501 tgaccattcc atgtggttcc atcgcccgtt taatttgaat gaatggctgc tgtatagcgt
     1561 ggagagcacc tcggcgtcca gcgcacgcgg ctttgtgcgc ggtgagtttt atacccaaga
     1621 cggcgtgctg gttgcctcga ccgttcagga aggggtaatg cgtaatcaca attaaaaaaa
     1681 tagccggagg tgaaaaccgt ccggctattt tttgcagtgc ttgttacgcg ttataggcat
     1741 tctcgccgtg gctgttgacg tccagccctt ctcgctcttg ttcttccggc acacgtaggc
     1801 caaccgtcag atccgccagt ttgtagccaa taaatgccac cacaccggac cagacgatcg
     1861 taatggcgat gctttccaac tgtaccagca actgatggcc catcgtcaca ccttcagcga
     1921 agcccacgcc gcccagagaa ctggcggcaa aaatcccggt catgatacag ccgacaatgc
     1981 cacaaacgcc gtgcacaccg aagacatcgc agggatcatc cacccgcagc aagcgtttga
     2041 gcatggtaac gccccacaaa cccgccagac cagctaccac gccgataatc aacgcgccgc
     2101 caaccccaat gtagccgcac gctggcgtca cgccgaccag accggcaatc gcgccagaac
     2161 acgctcccag cagagaaggc ttaccacgca gcgcccattc accgaagatc cagccaagaa
     2221 ttgccgccgc cgttgcgacc acagtattca caaatgccag tgccgcgatt tcattcgccg
     2281 tgcccactga cccggcgtta aagccaaacc aaccgatata gagaatggca gtcccggtga
     2341 agaccatcgg caggttgtgc ggtttaaacg cctctttacc gaagcctacg cgtttgccta
     2401 tcagatacgc gcccaccaga ccggcgattg ctgcgttaat gtgcaccacc gtgccacccg
     2461 cgaaatccag cgcaccgtga gaagccagca aaccaccgcc ccacaccata tgcgcaatcg
     2521 gaatgtaaga gagcgtcagc cataccacca cgaaaatcaa cacagctgag aagcggattc
     2581 gttccgccag cgccccaact atcaagccta cggtaatgca ggcaaacgat ccctgaaacg
     2641 ctacgtggat atactgataa atgctgccca tcaccgccgt cagttcgatg ttttttagca
     2701 tcaaccagtt aatgttgccg aagaagttgt tgccctcacc aaacgccagc gaataaccgt
     2761 aaaccaccca gagaatacag accagtgcaa acgtcaccgt cacctgcgtc agcatcgaca
     2821 gcacgttttt gccgcgaatc aacccaccgt aaaacagggc aatccccgga atagtcataa
     2881 acagcaccag cgcagtacaa atcatcataa acgcattgtc ggctttatcg gccaccgcag
     2941 gtgcagccat taccagtccc ggcagcatcg ccagtgaagc aagcccagtt tttatcgtcg
     3001 ctatcttcat ttttcgttcc tgttgctgtg tgccagagat tacagcgccg cttcgtcggc
     3061 ttcgccggta cgaatacgaa tgacgcgttg caattcagcg acgaagattt tgccgtcgcc
     3121 aatttttccg gtgtaagccg ccttactgac gatatcgatc acttcatcga gttggtcatc
     3181 agcaatcgcc acatcaattt ttacttttgg caggaagttg acgctgtatt ccgccccccg
     3241 gtacagctcg gcatgccctt tctgacgccc gaaacctttc acttcggtga cggtcaggcc
     3301 ctgaatacca atggaagata acgcttcacg aacgtcttcc agcttgaatg gtttgattat
     3361 cacggtcacc agcttcatag atcccctccg gtcagaattc ggtaatggtt ctgctacacg
     3421 aagaaggtat tgcaagcggt gtgccagaaa tgaaaaacga gaaagagagc aggaagttaa
     3481 gaagattgaa aatgaaaacg cactatgaca gtgcacagtg cgttacattt ttgcaccaac
     3541 gatgaaatat tgcgctattc aggcgctcaa tgattcctct tcacgcacgc tggctgccag
     3601 ctcttcgccc gcaagttgca gttgatacat ctgccagtag cgtccctggg ccgccagcag
     3661 ttgctggtga gtgccctgct ccacggcttg cccacgatga agcaccagaa tggtgtcggc
     3721 atcaataata gttgataagc ggtgagcaat caccaccagc gtggtatgtt cacgcaccgc
     3781 cgccagagca tgttgaatcg cctgttcagt accggagtca atgctggcgg ttgcctcatc
     3841 aaggatcagg atttgcggcg tctccaccag cacgcgcgcc agtgccagca gttgcttttg
     3901 cccgactgag agattatttc cctgctcgcc cagcggcgta taaatacaat cactcatgct
     3961 gcgcgccagc tccgccagtt gcacggtttc cagcgcctgc cagacgcgtt cttcggagat
     4021 atcccgcccc agcgtcacat tagcgaggaa ggtatccgcc agcaccaccg gatcttgctg
     4081 caccattgcc acgccctggc gcagcgcgct atgacttagc gaacttaatg gacgaccatc
     4141 aaggtgaatc tcaccttccg ttagcgggta atagcccatc aataaactgg cgagggtact
     4201 tttaccactg ccggtatgcc cgaccagcgc cacaaagtta cgcgaaggca cagagagatt
     4261 aatgttcttt agcaccagat tgtcatcgcg ataagcaaat gacacgttat cgacttcgat
     4321 ggtgccactc tgtaacgggt aatcatcatt gccatattgc tggcgcggtc cgtccatcag
     4381 ttcaaacacg cgctcaccag caacaacagc ctgttgcagc atcgcctgtt gcgtggtcag
     4441 ttcgattaag ggttcgttaa gtcgcccaag atagctgata aacgcataca acacgcccac
     4501 ttcaatggtg ccgctggcgg agaagccaaa cagcatcaat aagccacaaa gaatgagcga
     4561 tgaaaacaga ctcagcagcg gacgcagcag aaaaccgtcg aggcgcaggg tttgcatcct
     4621 agccatatag tgtgaacgac tggcctcccc catacgttcg ccaaatcgcg cctgctgacg
     4681 aaactgctgg ataacgctca tgccattgat gatttcgtta aagccgtcgt tgatatccgc
     4741 cagataggcg cgcacacggc ggacaatcgg cgtgctgtaa cgctggtata tcaccattac
     4801 caccagcacc accgggaaaa tcattatcgc caccagcgcc attcgccagt cgaggctgaa
     4861 catcgccacc agcatcgcgc ccaccagtgc ggcactgcgc aggacagttg ccactacggt
     4921 aacgtagaga tcgcggatca cttcagtgtc attagtgacg cgggaaatca cctgcccgac
     4981 gggttgggta tcaaactcgc ttaatggctg gcgtaacgca gcatccatca cgtcggtacg
     5041 caactgttgc actgcgccta ctgccgcccg attaaacagc agcgactgcg cgtaatgtag
     5101 cccggcggca aacagttgta gcccaacata cgccgcagcc agccctgcaa ccactttcaa
     5161 cggcaggtta tttttcgcta ccatattgtc gataaaatag ctgataagca gcggcccact
     5221 gacttctgcc gccgccgcaa cccacatcat caggaccgca atccccagcg gtttacgcca
     5281 cggcgaacca tacgctaaca ggcgcttgag agtcggccac agttggctaa aactacgcat
     5341 cgacggcctc ctcgcgaatt tccggaacgt cgtcgagcgc cgcctccagt tgttgatagc
     5401 gatacatatc gcgataccaa ccgctttgtt gcgccagctc atcatgattg ccacgctggg
     5461 cgatatgtcc gtgctgcatc acaataattt cactggcttc cgtcagtgcc gaaaggcgat
     5521 gggcactgat gattaccgtt cttccctgtc cccactgacg caggttatgc aggatctggt
     5581 gctctgtgcg tccgtccacc gccgaaagcg catcatcaag gatgaggatt tccgcgttga
     5641 ctaataacgc tcgagcaatg gagatacgct gtttttgccc accggaaagc atcacgccgc
     5701 gctcgcccac ttctgtatcg taaccttgcg gtagacgcaa aatatcgtca tgtacgctgg
     5761 ctaaccgcgc gacatgctca atctcttgct gggtggcatt cgggcaaccc agcgcgatgt
     5821 tattcgccac ggtgtcagaa aaaaggaatg gcgtctggct gaccaccgcc aggcggctac
     5881 gccagctatc gagttgtaac ttcgtcagag gaatatcatg aaagcgaata tccccctcgc
     5941 tgacgtcgaa atgacgctga atgagcgaca acagggtact tttgccggaa ccagtcggcc
     6001 cgcaaatacc cagcatctga ccgggtttca gggcgaaatt gacgttttcc agcgcaggat
     6061 ggtcagtctg cggatacgtg aactggtgaa tatttacatc cagttcgcca cgcccttccg
     6121 gcacgggttc gctaccatcg ttcaccaccg gcgcttccgc cagcatggcg cgaatacggc
     6181 tgtatgcagc actaccacgt tccacaatgt taaacatcca tgccagcgcc agcattggcc
     6241 aaatcatcag acctaaatac atcataaaac tggtgagctg gcccagcgtt aaactgccct
     6301 gcaccaccat ccaactaccg ccgccaatcg ccagcaagtt cgccatacca atcgcgatat
     6361 agatggtcgg gtcgaagcga gcatcaatac gcgccacccg catgtttttt ttgccggtat
     6421 cttcggcatc cgcggcaaat aacgccgact ggcgatcttc cagaccaaag gctttgatca
     6481 tgcggatact ggtgaggctt tcctgggtgc ggtcattaag actggaaaac gccgcctgtg
     6541 ccagcttaaa gcgttcatgc aaggcatcgc cgttgcgctt aatcatgatc gccatcactg
     6601 gcatcggtaa cagggcaaat aaggtcaact gccagctaat ttgcgtagac atcataatca
     6661 acacagcgca gcccattacc agtgaatcca ccagcgtcag caccccttct ccggcggcaa
     6721 acacgacgcg atcgacgtca ttggtcgcac gagccatgag atcacccgtg cgatgacgca
     6781 ggtaaaactc cgggtgctgc cggcttagct gacggtaata atcttcacgc agttcaacag
     6841 ccagttggta agacgcgcca aacagcaata cgcgccagac ataacgcagg agataaacca
     6901 caacggcaat cagcaccatg gtggcgatcc acatcaggct cagcccggta gtaaagtgtt
     6961 gttctgtcac gccatcgaca acaataccaa ccacttttgg cggaaccagt tgcagcatcg
     7021 cgataatgac aagcaaggcg acagccccaa gatagcgacg ccattcccga cggaaatacc
     7081 agcttaattg agcaaataat cgcacgcgtt gtgtcctgac ctgattctgg atatttattc
     7141 gatgggtaaa gaagtggtgt atttaatctg ttccatcgcg aagctggaag tgacgtccga
     7201 cagccccggc acgctgttta ccagacgctt ataaaactcg tcgtagcgtt tcatgtcggc
     7261 aacctggacg cgcatcagat aatcgtattc accagccatg cgccagaccc ccagcacttc
     7321 tggcatttcg gtaaccaccg tgacaaagcg gcaataccat tcgctgctgt gatgttgcgt
     7381 ttttatcagc acaaaggcgg tcaggccgag gcctattttt tccggatcca gcagggcgac
     7441 tttgccgata aggataccgt cgtcctccag ccgtttcagg cgcttccagc agggggtggt
     7501 tgtcagatta acggcttcag ccagtgcctg caaagagagg gtgcaatcct gctgcagtaa
     7561 ggccagcagc ttacggtcaa ttttatctaa catagccctt ccacggagaa tttttttctc
     7621 ttttaattct attttaaagg tgaaatcgcg acaatttatt ctgtgcggtt taaacaccac
     7681 ggcacaaaat gacaaattca acgcaaaaaa aaaccgggca attgcccggt gctggaaggg
     7741 atctcgttat tcgggggaat aaggtagatg tggatagtcc agccagtgcg tcaaatagtg
     7801 agagacagcc tgatttcggc aatgtccaat caccggtaaa tgcgggagct ccgcgcgcag
     7861 ttgcggcatc gcattgccca taataaaccc gctaccgacg ctgcctaaca tttcgcgatc
     7921 gttcatcgca tcaccaaagg ccatgcaatc gcgcaacgat aaacctaaat gttgggtccg
     7981 caccgtcaat gcagcgcctt tattgcagcc caccggcagc acttcgaggc aatccgtggc
     8041 ggaaaaacac aaatgtgcac gctcgcctaa tgcttcgtat agctggatct gcaagcgtgt
     8101 aagatcgtcg tgatcgccac agaagcagat cttggtgacg ctgccgagtg gcattttttt
     8161 gacatcgatt atctgataac gaaaaccgct atagacaaat acctgcaaca acgcagggct
     8221 ctctttcccg gtaaaccaac cgtcgtcatt gaagatatgc atgctggctc gggtatccca
     8281 ttgctgatac agcaccagct ccgcgacatc cgcaggtaaa tcatcacgat gtaaaagttc
     8341 accttccaga gaatgcacgc gcgttccgtt gccggtaatc aaatatgcat ccagcgatag
     8401 cgccccgaga atatgctgca tctccagcgc atgacgcccc gtggcaaaag tgagggtaat
     8461 gtcgcgttca cgcagtcgcg ccaaagtaga gagggttttc tcacctaaat gatggtcagg
     8521 catcaataaa gtgccatcca tatcaaatgc taccagacga gccatttctt tctccactct
     8581 gtgacacggt taatggtgat tgagtatcac ctgatatata cggaagtaat agtgaatagt
     8641 taaataatat tgttccgggt ttatatgcga ttgctcaacc gtcttaacca gtatcaacgt
     8701 ctgtggcaac cttcagccgc aaaccgtcac cgtcagcgaa ctggccgaac gctgtttttg
     8761 cagcgaacgc catgttcgta cgctgttgcg tcaggcacag gaggcgggat ggctggagtg
     8821 gcaggcgcag tcaggacgcg gaaagcgcgg acaattacgc tttctggtca cgccggaatc
     8881 gctacgcaat gcgatgatgg aacaggcact ggaaaccgga aagcagcaag atgtgctgga
     8941 gctggcgcaa ctggccccag gtgagctgcg cactctgtta cagccattta tgggcggaca
     9001 atggcaaaac gatacaccca cgttgcgtat tccctactat cgcccgctcg aaccgctaca
     9061 accaggcttt ttgcccggcc gtgccgagca gcatctcgcc gggcagatat tttccggcct
     9121 gacccgcttc gataataata ctcagcgccc gattggcgat ttagcgcatc actgggaaac
     9181 ctctactgac aggttacgct gggactttta tcttcgttca accctacact ggcataacgg
     9241 cgatgcagta aaagcctcac acttacacca gcgattattg atgctgttac aactgccagc
     9301 actggatcaa ttatttatta gcgtgaagcg tattgaagtc acccatccgc agtgtctgac
     9361 cttcttttta catcgccccg attactggct tgcgcaccgg ctggcgagct attgcagcca
     9421 tctggcgcat ccgcaattcc cactgatcgg cactggtcct tttcgcttaa cacaattcac
     9481 agcggagctg gtgcgcctgg agagccatga ttattaccat ttgcgtcatc cgctgcttaa
     9541 agcggttgag tactggataa ctccgccgct tttcgaaaaa gatttgggaa ccagttgtcg
     9601 gcatcccgtg caaatcacca tcggcaaacc ggaggagctg caacgggtca gccaagtcag
     9661 tagtggcatc agtttaggtt tttgctattt aacgttgcgc aaaagtcccc gactctccct
     9721 ctggcaggcg cgaaaagtga tctccattat tcatcaatcc ggtttattac aaacgttaga
     9781 agtcggagaa aacctgatca ccgccagtca tgcattactg ccaggctgga ctattccgca
     9841 ctggcaagta ccggatgaag tcaaactacc gaaaaccttg acgctggttt atcacctacc
     9901 gatagaactt cataccatgg cagaacgcct acaggcgaca ctggcagcgg aaggctgtga
     9961 actcacaatt atttttcata acgcaaaaac tgggacgaca cgaccctact ggcacacgca
    10021 gacctcatga tgggcgacag attaattggc gaagcaccgg aatatactct ggagcaatgg
    10081 ctacgttgcg atccactatg gccacatgtt ttcgacgctc cagcatatgc acatctgcaa
    10141 tcgacgctgg acgcggtgca agtaatgcct gatgaggaaa accgatttaa tgccctgaaa
    10201 gcggttttta gccagttaat ggcagacgcg acgctgacgc cgctgttcaa ctatcactat
    10261 cgcattagtg cccctcccgg cgtgaacggt gtgcgactga caccgcgcgg ctggtttgaa
    10321 tttaccgaag cctggcttcc cgcgccgtcg caatgaatag ctggtccggg ttaatcgcag
    10381 gcgttaccat aacgctttta ttgttcaagc aggattatct atgaaacgtg ctgtcgttgt
    10441 gttcagtgga ggccaggatt ccaccacctg tctggtgcag gcattacaac aatatgatga
    10501 agtccattgc gtgacgttcg attacggtca gcgacatcgc gcagaaatcg acgtggcacg
    10561 cgaactggcg ctgaaactgg gagcacgcgc gcataaggtt cttgatgtaa cgctgctcaa
    10621 cgagctggcg gtcagcagcc tgacacgtga cagcattccg gtgcctgatt atgaacctga
    10681 agccgatggc atcccgaata cgtttgtccc agggcgtaat attttgttcc tgacgctggc
    10741 ggcaatatat gcgtatcagg taaaagcaga agccgtaatt accggcgtct gtgaaacgga
    10801 tttctccggc tacccggatt gccgcgatga gtttgtgaaa gcactaaacc atgccgtcag
    10861 tttgggcatg gcgaaagata ttcgttttga aacgccgctg atgtggattg ataaagcgga
    10921 aacctgggcg ctggcagatt attacggcaa actggattta gtccgtaacg aaacgttgac
    10981 ctgctataac ggcattaaag gcgacggttg cggtcattgt gcggcatgta atttacgcgc
    11041 caacggtttg aatcattatc tggccgataa accgacggtg atggcagcga tgaagcagaa
    11101 aaccgggttg aggtaattat tccgggtgtc gccggatgcg gcttgagcat ccggcaccac
    11161 aaaacgttta cttaaccatc tgctccagct tttcgcgcaa ttccccttcc agagctaatg
    11221 ctttctgcgt tttaagatca atacaaacaa acgtaataag cgcatccgct accacctgcc
    11281 cttccggctc cagtgtaatg acctggttta agatgccgct tttaccgttt aattgctgca
    11341 actgactggt aatagttaac aggtcgctta ataccgctgg acgacgatag ttaatattga
    11401 tattgaccac gacgaaggcg atgttatggg ccgtcatcca ctgaaaactg tcgctatttt
    11461 ccaccccatc ccagcgggct tcttcgagaa attcaaggta gcgggcgttg ttgacgtgct
    11521 ggtaaacgtc gagatgatat ccacgaactt tgatttgtgt ttgcatagcg caataacctt
    11581 acgttgttgt tataagtaca gaggtcataa ctggtatgac ctcttcagtc tggcaaaaaa
    11641 ttgccactat gcaaattaat tacagggtta ataccgccag attacgttcc accagcgaat
    11701 tgcccatccc cggcacctgc tttagatcct ccaccgtttt aaacggaccg tactcttcgc
    11761 gataactgac aatcgcctgc gctttcttca ggccaacgcc attcatcgcg cgggctagct
    11821 cttccgcgct ggcattatta atgctgaccc gggtgccttc ttcgtcactg gctttcgccg
    11881 gtactgctgc tttactttgt gctgcaggag cttccgcttt ggtttctacc gccgtcggtt
    11941 tcgccacaga agctgccgcc agcgcgctat gagacattcc ggcacaggcc agggacagtg
    12001 taatgagcag tgctttaatt ccgtgtttca tactgttttc tccttgtttg ttaacagtgg
    12061 aatcacctta acgggagtga aaacagtaaa caaatggcaa tgttcagaaa tggaaaaggc
    12121 cgcgaaagcg accttttctt gttacagctg caacgcattt ttttgcgagg cggcttcagg
    12181 attattgctg ttccagcgca tcgccaattt tgattttcgc ctctttacgc aggttactca
    12241 tcagagcttc aaagacgatt tgtgcgttgt tctgggtgat accctgcacc atcgcttttt
    12301 tctgatcttc cggcattgaa ccttgtttca cttcatccag cgccagcaga accacattac
    12361 cttgcatatc ggtcgccata ccgtagctcg gtttgtcttt cgctggcagt ggcagtgcaa
    12421 acgccgcctg gctaatcggg tcacgaccgg aacggcttaa ggttttcggc tcgccaaatt
    12481 tcagaccggc agcctgcata gcttccgcac ctttgccggc tttcaaatca accagcagtt
    12541 tctcagcatc cactttcgcc tgttgttcag ctttgttgtg ctgaaccagt gccttaactt
    12601 gttcctgaac atctgccaac ggtttcaccg cttccggttt gtgctcgctg acgcgcagca
    12661 cgaatgcgcg gtcgccgtct acggtgatga tgtcagagtt gatgcccgac gcgccgtttt
    12721 cacctaccag accgccgtta aagatagcgt cggcaaccgg cttgaagttc aactcttccg
    12781 gcaggttatc tttgctgaac caacccgtct gagtggcttt aacgccggca gcttgctctg
    12841 caccggccag agactcggtg tcgttgcttg ccgcatcgct cactttctgc tgcagcgcat
    12901 agtacgcatc gagggctttt tcgtgtttca ctttcgccgc aatgtcgtca cgtacttcgt
    12961 ctaacgattt cactttcgct ggctgaatgt cgtccagacg tacaatcagg aaaccgaccg
    13021 aagatttgat gacaccagac agttggcctt tttctttcag accagcattt ttcagttcgt
    13081 cggggatagt ggcatcttct aaccaaccca tatcgccgcc gttacgggca gagataatat
    13141 cggcagattt ttctttggct aatgcagcaa aatcaccgcc tttattcagc tcatcaagta
    13201 ccgctttcgc ttcatcttca gttttggtct ggatgatgct gtagcgggta cgctgcggct
    13261 gggtgaattg atcctgatgc tgatcgtagt agctctggat atccgcatcg ctaaccggtt
    13321 gctgcatcgt tgcggcaacc agcttgatgt aactcacgcg gaattgttcc ggcgtcatga
    13381 aattgttttt gttttgttcg tagtagctgg caatctcctg ttcggtcaca ggctgcttcg
    13441 ccgccagcgc gttaacatcg atagtcgcct cacgcaccac gcgttgttgc gcgaccagtg
    13501 ccgccagctc gtcggtttca cctttcagca taaaatcggt accggcaacg ccgttaatca
    13561 gctgttgggt agtgagctgg ttacgcagcg cctgggcgta ctgatcggcg gtcatcccca
    13621 tctggttgag gataccgtta tagcggctgt tatcaaattt gccgtcaacc tggaaggctg
    13681 gggtcgcgaa aatcgcctgt ttaacctgct catcactgat acccagtttc agctcacgag
    13741 cgtactgatc caacagcgcc tcgtcgatca gacgattcag cacctgttga cgcagggttt
    13801 tcatatagcc ttcgttcgct gccagctcgg agtattgatc gcccagctgt tgctgcatac
    13861 gattacgctc gctgttaaag gcgttctcga actgcccacg gctgatttcc tggtcattca
    13921 cttttgcggc gtagttattg cctccgccaa tcaggtaacc actcacgccg gtcaatatga
    13981 acgacacgat aatgataccg aaaataatct tgagcacgag actgtttgca gccgtgcgta
    14041 agctgtccat catggtgtaa caacactccg ctgtagatga ctgtgtacct cacgcaacgt
    14101 atcgatgcgc gcaaaggcct attgtgacaa gaaacggggg caattgtcag cccacaagac
    14161 acagaaagtc accgaataca aataaaaaag gcacatcagt agatgcgccc ttgaacttcg
    14221 tcacatcccc actggggaca acgcttagtt taccgcgtct ttcagtgctt tacctgcacg
    14281 gaagctcggt actttagcgg cagcgatggt gatctcttta ccggtctgcg ggttgcggcc
    14341 agtacgggca gcacgctctt taacggcaaa agtaccaaaa cctaccagtg ctacatcatc
    14401 cccttctttc agagattcag ttacggaagc aataatagca tctaacgcac ggccagccgc
    14461 agctttagag atatcagccc ctgcagcaat cttgtcgatc aattgagatt tattcactct
    14521 tctcttcctc tttataattt atatcgcact tgaatccttc aaggtacgaa cgcgcagcag
    14581 ttatatcagg ccagccatcc ccttacaaca ccccttaata agggcaagcc cgatccgcta
    14641 tctaacttag cgagacaaaa aaaggctggc aagcccgaat tagcctgcca gccctgtttt
    14701 tattagtgca ttttgcgcga ggtcactatt ttgcagtcac aacctgcatg ccagacggtt
    14761 cattttgcag cgccagagtc agaacttcct caatgcgctt cacaggatga atgtccagat
    14821 cggcaattac gttgtcagga atctcttcca gatcgcgttt attttcgaac ggaattagca
    14881 ctgttttaat cccgccgcga tgcgctgcca ggagtttttc tttcaaacca ccgatcggca
    14941 gtacctgacc acgcagagtg atctcaccgg tcattgccac atcggcacga accgggttac
    15001 cggtcaggca agaaaccagc gcggtgcaca tagcaatacc ggcactcgga ccatctttcg
    15061 gcgtcgcacc ttccggtacg tggacgtgga tgtcacgttt ttcgtaaaaa tcagggttga
    15121 tccccagttt ttccgcacgc gcacgaacca ccgttaacgc cgcctgaata gactcctgca
    15181 tcacttcgcc gagcgaaccg gtataggtca gtttgccttt acccggaaca catgcggttt
    15241 caatggtcag caagtcaccg cccacttccg tccacgccag accgattacc tgaccgacac
    15301 ggttttcgtt atcagcgcga ccatagtcga aacgctgaac accgaggtag tcatgcaggt
    15361 tatcgccgtt aatttcgata tgttttaatg acttatcgag cagtaactgc ttaaccgctt
    15421 tgcggcacag tttggagatt tcacgctcca gaccacgcac gcccgcctca cgggtgtagt
    15481 aacgaataat gccgataatg gcgctatcgt cgacggtcag ctcacctttt ttcagtgcat
    15541 tacgttcaat ctgcttcggc agcaggtgac gtttggcgat gttcagtttt tcatcttcgg
    15601 tataaccgga gaggcgaatc acttccatac gatccagcag cggtgccgga atgttcatgg
    15661 agttcgacgt cgcgacaaac atcacgtcgc tgagatcgta atccacttcc aggtagtggt
    15721 cgctgaacgc tacgttctgc tctggatcca gcacttcaag cagtgcagag gccggatcgc
    15781 ctcgcatgtc agaagacatt ttgtcgatct catcgagcag gaacagcgga tttttcacgc
    15841 ccactttcgc cattttctgg atcagtttac ccggcataga accgatgtaa gtacggcggt
    15901 gaccacggat ttccgcttca tcacgcacgc cgcccagcgc catacggaca tatttacgcc
    15961 cggtggcttt ggcaatggac tgaccaagag aggttttacc tacccccggc ggccctacca
    16021 ggcagaggat cggtcccttg attttgttga cacggctttg aaccgcaagg tactcaagga
    16081 tgcgatcttt cacgcgctcc agaccataat ggtcggtatc aaggatttcc tgcgcctgac
    16141 gcaggtcttt tttgaccttg ctgcgcgcat tccacggtac ctgtaccatc cagtcgatat
    16201 aaccacgcac tacggtcgct tctgccgaca tcggagacat cattttcagc ttctgcaact
    16261 ctgcttccgc tttctctttt gcctctttcg gcatcttcgc cgcgtcgatt ttgcgcttca
    16321 gggcttcgtt ttcgtccggc acgtcgtcca tttcaccgag ttctttctga atagctttca
    16381 tttgctcgtt cagatagtac tcacgctggg atttctccat ctgcttttta acgcggttgc
    16441 gaatgcgttt ctcaacctgc agcagatcga tttccgattc catcattgcc atcagatatt
    16501 ccagacgttc gttaacgtcg gacatctcca gaacggactg tttgtcagcc agtttcagcg
    16561 gcatatgtgc agcaatggta tccgccagac gcgccggatc gtcgatgcta ttcagcgacg
    16621 tcagcacttc tggtgggatt tttttgttca gcttgatgta gccttcgaac tggctgattg
    16681 cagtacgcac cagcacttcc tgttcccgct catcaatggt cggcgactcc agatactccg
    16741 ccttcgcaga aaagtgttcg ccattgtcag agagcgcaga aatacgcgcg cgctgtaacc
    16801 cctcgaccag cactttgacg gtgccgtcag gcagtttcag catctgcaat atagaggcca
    16861 cggtcccgac ggtgaaaaga tcgtttacac ccggctcatc cgttgaagct tctttctgcg
    16921 cgaccagcat aattttttta tcatggtcca tcgccgcttc cagacaacgg atagattttt
    16981 cccgcccgac aaataagggg atgaccatgt gcggataaac caccacatcg cgcagcggca
    17041 atacggggat ttcaatgcgt tcagaacgct caggattcat agagctctct cttagtttaa
    17101 tttccgccag gtaatcagat gacacgactg tgcttcacgc catctattaa catgtacgtc
    17161 agtatatggg gatgtttccc ccacattcaa cgccgagaat agaggaaaaa ttaaagggga
    17221 gataaaatcc cccctttttg gttaactaat tgtatgggaa tggttaatta ttcaccagat
    17281 gcctgttgcg cttccggctt accataaatc agcaacggtt tgctttgacc atcaattacc
    17341 gactcgtcga taaccacttt ttcgacgtct tccatggacg gcagatcgta catggtatcg
    17401 agcagtgcgg cttctacgat ggaacgcagg ccacgggcac cggttttacg cgccatcgct
    17461 ttcttagcga tagcatccag cgcctcgtca cggaattcca gatccacgcc ttccagatta
    17521 aacagcgcct gatactgctt ggtcagggcg tttttcggct ctttgaggat ctgaatcaga
    17581 gcttcttcgc tcagttcatt caacgttgct acaaccggca gacgaccaat gaactcaggg
    17641 ataagaccaa acttgatcag atcttccggt tcaacctgcg ccagcagctc gccttcgctt
    17701 gctttgtcgg acttcgcttt taccgtcgcg ccaaaaccaa tgccggagcc ggtttctaca
    17761 cggtgggaaa tcactttatc cagaccggca aacgcaccgc cacaaataaa caggatctta
    17821 gaggtatcaa cctgcaagaa ttcctgctgc ggatgtttac gcccaccttg cggtggaaca
    17881 gcagctaccg taccttcgat cagtttcaac agtgcctgct gtactccttc accggaaacg
    17941 tctcgggtaa tggacgggtt gtctgactta cgagaaatct tgtcgatttc atcgatgtag
    18001 acaatcccgc gctgcgcttt ctgtacgtcg taatcgcact tttgcaacag cttctgaatg
    18061 atgttttcaa cgtcttcgcc cacataaccg gcttcggtca gcgtggttgc gtcggccatg
    18121 gtgaacggga cgtccagcag gcgcgccagc gtttcagcca gcagcgtttt accggaaccg
    18181 gtcggaccga tcagcagaat gttacttttg cccaactcga caccattgct ggtatcgccg
    18241 ttgcgcagac gtttgtaatg gttgtatacc gcgaccgcca gcactttttt cgcctgttcc
    18301 tggccgataa cgtaatcgtc caggtggttg cgaatttcat gcggcgtcgg tagcgcactg
    18361 cgttcacgat gcggtgcaac ttctttaatc tcttcgcgaa tgatgtcgtt acataaatca
    18421 acacattcgt cgcagatata cacggatgga ccggcaatca gcttgcgcac ttcatgctgg
    18481 cttttgccgc aaaaagagca atacagcaat ttgcctgagc catctttgcg tttatctgtc
    18541 atgggtcaaa acctcttctt tgttctttgt gccgcacacg acgacgcaaa tgccattctc
    18601 aggcgcaagc cgcttacagc gttgtgccgc cctagataag tatagcggca cagttgcgcc
    18661 tctggcatca attacgatgg gtcagaatcg aatcgaccag accgtattcc accgcttcag
    18721 gggcggaaag gaagcgatcg cgctcggtat cacgttcaat ctgttctaat gattgacccg
    18781 tatgaagcgc cataagttca ttcatgcgcc ctttaacttt cagaatttca cgggcatgaa
    18841 tttcgatatc ggtcgcctgg ccctggtagc cgcccaacgg ttggtgaatc atcacgcgcg
    18901 aattcggcag gcaaaaacgt ttaccttttg cccctgcggt caacaagaaa gcgcccatcg
    18961 aggccgcctg gcccatacag atggtgctga catcaggctt gataaactgc atggtgtcat
    19021 agatagacat cccggcagtg atcaccccgc ctggggagtt aatgtacaga tagatatctt
    19081 tttctgggtt ttccgcttcc aggaacagca tctgcgccac aatcaggtta gccatgtggt
    19141 cttcaacctg gccagtcaga aaaatgacgc gttccttaag tagacgagaa tagatatcaa
    19201 aagagcgctc accgcgtgag gtctgttcaa tgaccatcgg caccagcgcc atatggggtg
    19261 caaagttatc tcgttcgccg ctgtatgaca tttccgtctc ctggataaaa ttgaaaaaaa
    19321 cctgctgtac cgattgtaac cttatggacg gattatcagc tagtccctgt gttatgggta
    19381 cggcatcacc atatttcaag cataacaatc ttttgttgtt acgctaacac cgaaagaggc
    19441 ttttcgcacg gctccatgca aaattcatga caaaaaaaag cccaccacct ggcggtgacg
    19501 ggcctttgtg cgaatttagc gcgttatgct gcgtaaatta cgcctgctgg ttcatcagct
    19561 cgttgaaagt ggtttctttt tcagtcactt tcgctttcgc cagtacagct tcaacagcct
    19621 gttcttccag agcaacattg cgcatgttgt ccatcagttc tttgtttttg ctgtagaact
    19681 cgataacttc tttcggatct tcgtacgcag aagccatctc ttcgatcagg cctttcacgc
    19741 gctcttcgtc agctttcagc tcgttggtgc ggataacttc gcccagcagc aggccaacaa
    19801 ctacgcggcg tttagcctgt tcttcgaaca gttcgcgcgg cagttccaga gcttgttttt
    19861 cgttgccacc gaaacgctgt gcagcctggc gacgcagaac gtcgatttcg ctgtcgatca
    19921 gcgcagccgg tacgtcgatg tcgttagctt ttaccagacc ttcgatcgcc tgagacttaa
    19981 cgcggttacg gatggcgctc ttcagctcgc gctccatgtt tttacgcact tcagcgcgca
    20041 gaccttctac ggaaccatct tcaacgccga aacgtttgat gaattctgca gtcagttccg
    20101 gcagttcacg ctcttcaact ttcttcaggt tgatagcgaa tttcgctgct ttacctttca
    20161 ggttttctgc gtggtattct tccgggaagg tcacgtcgat ggtgaactct tcgccagctt
    20221 tgtggccttt gataccgtct tcaaagcccg ggatcatacg accctggccc atcgccagta
    20281 cgaaatcaga cgctttaccg ccttcgaact cttcgccgtc tacagaaccg gtgaagtcga
    20341 tggttacgcg gtcttctgct tcaacagcgc cgtctttttc tttccaggtc gcctgctgtt
    20401 tacgcagagt atccagcatg ccgtcaacgt cagcgtcggt cacttcaacg atcggttttt
    20461 caacttcgat cgcttccaga ccctgcagtt caacttccgg ataaacttca aactctacag
    20521 agtaagtgaa gtcttcaccc agcttgtatt cgcccggaac ataagtcggt gcgccagccg
    20581 gattgatttt ttctttaatg atggcgtcaa tgaagttacg gctcatcagg tcacccagaa
    20641 cgtcctggcg tacagacgcg ccataacgct gagcaacgat attcattggc actttgcctt
    20701 tgcggaagcc gtcaatacgt acttttttcg caacgttgac cagctcgctt ttaacagcgg
    20761 tctcgatgct gtcagcagcg atagtaatcg ttacacggcg gccaaggcct tgagtggttt
    20821 caactgaaac ttgcatcttg ttacctcaaa aaatcacagt gctcggtcaa ctcttacccc
    20881 gcaagcacag gttgttggct tcgcggaacc gggatgttct tataatcaat cacattccct
    20941 gttaccagaa tcatcccgaa agcattcaaa taggacgcgg cattatagcg gcatcacttt
    21001 tatgagtcga gaacggttat tgcgcgctgt cgcgtatttt ttcacaattc ccgggcaatt
    21061 ttcgtctgaa tgctgggcaa aaagcgcaaa atccagacaa aacaaaacgg cccgcaggcc
    21121 gtttttcata aaatctttag caacatactg gaaaagcgcc gacagttgca aatgcgtttt
    21181 tacgcgatgc ttcctgctcc acgacaggga ggagaggcaa agacggtgtc ctgcaaccct
    21241 tcccactcct taatagtgta agtatgcaga gccagcgcat gaacggtagt agagagttcc
    21301 tccgctaaag tactgtaaat cattcgatga cgattcagaa aacgttcacc cgtaaaacga
    21361 tcgctgacca gcacaacttt aaaatggctt tcagagccgg ctgggacatt gtgacgatag
    21421 ctttcatcca ctacttcgag gaatacgggt tggaacgccg cccttaattt ttcttctatc
    21481 cgctcacgta tcatcatgaa atatctcctc cgacaacgct gagatgtcat ccatcccttt
    21541 aaatactagc cgcttttacc gtttccattg cagcttaaca acaaatattt aggtttcgtc
    21601 ctggtttttc cgtcaagcgt atgaagttta ctgatttaaa gattgaaaca caggcctcgc
    21661 atcagcgttt cttttaccag gggtgattat ttcgtcaaca tgatgaattt acatgcctta
    21721 cccacttccc ctcgccgttg gcaatgttat gatggcggga atttttacca aacacgctta
    21781 agagtctctg agagcccgaa catgttcaaa aaaatcctct tcccgttagt tgctctgttt
    21841 atgcttgcag gatgcgcaaa accgccaaca actattgaag tttccccgac gattacactg
    21901 ccacagcagg atccaagcct gatgggcgtc accgtaagca ttaatggtgc cgatcagcgt
    21961 accgatcagg cgctggcaaa agtcacccgc gataatcaaa tcgttaccct gaccgcctcc
    22021 cgcgctctgc gtttcctgct gcaagaagtg ctggaaaaac agatgaccgc gcgtggttac
    22081 atggttggtc cgaatggccc ggttaatctg caaatcatcg ttagccaact gtatgctgac
    22141 gtgtcccagg gcaacgtgcg ctacaacatc gctaccaaag cggatatcgc catcatcgct
    22201 accgcgcaga atggcaacaa aatgaccaaa aactatcgtg ccagctacaa cgttgaaggt
    22261 gcgttccagg cctccaacaa aaatatcgcc gatgcggtta acagtgtgct gaccgatacc
    22321 atcgctgata tgtctcagga caccagcatt cacgaattca tcaagcagaa cgcgcgttaa
    22381 tttctgccct ctggcccggt gcaagccggg cctgtagacg cccatgtcca gtcaatattt
    22441 acgtattttt caacagccgc gttcagccat attgctgatc ctgggttttg cttccgggct
    22501 accgctcgcc ctgacatccg gcaccttaca ggcctggatg acggtcgaaa atatcgatct
    22561 caaaaccatt ggtttcttct ctctggtagg ccaggcttac gtttttaaat tcctctggtc
    22621 accgctgatg gaccgctaca cgcccccatt ttttgggcgg cggcgcggtt ggctgctcgc
    22681 cacgcaaatc ctgttattag tcgccattgc ggcgatgggt tttctcgaac caggcaccca
    22741 actccgctgg atggcggcgc tggcggtggt gatcgctttt tgctctgcct cccaggatat
    22801 tgtcttcgat gcgtggaaaa ccgatgtgct cccggcagaa gaacgtggtg cgggcgcggc
    22861 aatcagcgtg ctgggttacc gtttagggat gctggtttcc ggcggcctag ccctgtggct
    22921 ggcagataaa tggttgggct ggcagggcat gtactggttg atggcggcgc tgttgatccc
    22981 ctgtattatc gcgacgttgc ttgcaccaga accaaccgac accattcctg tgcccaaaac
    23041 gctggaacaa gcggttgttg cacctctgcg agatttcttt ggtcgcaata atgcctggct
    23101 tattttgctt cttatcgtgc tgtataagct gggcgacgca ttcgccatga gcctgacaac
    23161 cacgtttttg attcgcggcg tcgggtttga tgcgggtgaa gtaggcgtgg taaacaaaac
    23221 gcttggctta ctggcgacca ttgttggcgc attgtacggt gggattttga tgcagcgcct
    23281 gtcgctgttc cgggcgctgc tgattttcgg cattttacaa ggtgcgtcta acgctggtta
    23341 ctggctgctg tcgattactg ataagcatct ctacagcatg ggcgcagccg tctttttcga
    23401 aaacctctgt ggcgggatgg gcacatcagc ctttgtcgcg ctgttaatga cgctgtgtaa
    23461 taagtcattt tccgctactc aatttgccct gctctcagcg ctttccgctg tagggcgagt
    23521 ttatgtcggc cccgtggcgg gttggtttgt tgaagcacac ggctggtcga cattctatct
    23581 attctccgtc gccgctgccg taccggggct tattttgctg ctggtttgcc gccagacgct
    23641 tgaatataca cgagtaaatg acaactttat ctcccgtacc gaatatccgg caggttatgc
    23701 ctttgccatg tggacactgg cggcgggcgt cagcctgttg gccgtgtggt tacttctgtt
    23761 gacgatggac gcgctggatt tgacgcactt ctctttcctg cctgctctgc tggaagtcgg
    23821 ggttttagtc gccctttctg gcgtcgtgct tggtggtttg ctggattatc tggcgctacg
    23881 aaaaacgcat ctgacgtaat ctgtaaatat tatttagctg tagtaatcac tcgccgtaat
    23941 tattacggct aaataaacat cagcatcgct tattaatatt tgttcttttg tgcggcttag
    24001 cgtttggaaa ttattgacgc catttataaa ttctattttt cttacacgat tcagctaatg
    24061 agtc
//
LOCUS       DAEBXN010000060        22852 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.060, whole genome shotgun sequence.
ACCESSION   DAEBXN010000060 DAEBXN010000000
VERSION     DAEBXN010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22852
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.060"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(89..625)
                     /gene="ssb1"
                     /locus_tag="KJL99_002446"
     CDS             complement(89..625)
                     /gene="ssb1"
                     /locus_tag="KJL99_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HBD4437141.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPYNEPPMDFDDDIPF"
     gene            879..3701
                     /gene="uvrA"
                     /locus_tag="KJL99_002447"
     CDS             879..3701
                     /gene="uvrA"
                     /locus_tag="KJL99_002447"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBD4437142.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3736..4092)
                     /locus_tag="KJL99_002448"
     CDS             complement(3736..4092)
                     /locus_tag="KJL99_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HBD4437143.1"
                     /translation="MTISELLQYCMAKPSAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4096..4512)
                     /locus_tag="KJL99_002449"
     CDS             complement(4096..4512)
                     /locus_tag="KJL99_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HBD4437144.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMECFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="KJL99_002450"
     CDS             complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="KJL99_002450"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HBD4437145.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            5738..5942
                     /locus_tag="KJL99_002451"
                     /pseudo
     CDS             5738..5942
                     /locus_tag="KJL99_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6445..7638)
                     /gene="tyrB"
                     /locus_tag="KJL99_002452"
     CDS             complement(6445..7638)
                     /gene="tyrB"
                     /locus_tag="KJL99_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HBD4437146.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEVTNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(7891..8970)
                     /gene="alr"
                     /locus_tag="KJL99_002453"
     CDS             complement(7891..8970)
                     /gene="alr"
                     /locus_tag="KJL99_002453"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBD4437147.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(9023..10438)
                     /gene="dnaB"
                     /locus_tag="KJL99_002454"
     CDS             complement(9023..10438)
                     /gene="dnaB"
                     /locus_tag="KJL99_002454"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBD4437148.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            10521..11504
                     /locus_tag="KJL99_002455"
     CDS             10521..11504
                     /locus_tag="KJL99_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HBD4437149.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDHVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGIGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(11670..11912)
                     /gene="pspG"
                     /locus_tag="KJL99_002456"
     CDS             complement(11670..11912)
                     /gene="pspG"
                     /locus_tag="KJL99_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HBD4437150.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(12046..13083)
                     /gene="dusA"
                     /locus_tag="KJL99_002457"
     CDS             complement(12046..13083)
                     /gene="dusA"
                     /locus_tag="KJL99_002457"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HBD4437151.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIEHELSQGTYLGHIIRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(13446..14431)
                     /locus_tag="KJL99_002458"
                     /pseudo
     CDS             complement(13446..14431)
                     /locus_tag="KJL99_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 domain-containing protein"
     gene            14749..15264
                     /gene="zur"
                     /locus_tag="KJL99_002459"
     CDS             14749..15264
                     /gene="zur"
                     /locus_tag="KJL99_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HBD4437152.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            complement(15306..15515)
                     /locus_tag="KJL99_002460"
     CDS             complement(15306..15515)
                     /locus_tag="KJL99_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HBD4437153.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(15631..16956)
                     /gene="dinF"
                     /locus_tag="KJL99_002461"
     CDS             complement(15631..16956)
                     /gene="dinF"
                     /locus_tag="KJL99_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HBD4437154.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            complement(17029..17637)
                     /gene="lexA"
                     /locus_tag="KJL99_002462"
     CDS             complement(17029..17637)
                     /gene="lexA"
                     /locus_tag="KJL99_002462"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HBD4437155.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(17747..18115)
                     /gene="dgkA"
                     /locus_tag="KJL99_002463"
     CDS             complement(17747..18115)
                     /gene="dgkA"
                     /locus_tag="KJL99_002463"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBD4437156.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            18286..20709
                     /gene="plsB"
                     /locus_tag="KJL99_002464"
     CDS             18286..20709
                     /gene="plsB"
                     /locus_tag="KJL99_002464"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HBD4437157.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RFYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(20864..21736)
                     /gene="ubiA"
                     /locus_tag="KJL99_002465"
     CDS             complement(20864..21736)
                     /gene="ubiA"
                     /locus_tag="KJL99_002465"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HBD4437158.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWIMAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(21749..22246)
                     /gene="ubiC"
                     /locus_tag="KJL99_002466"
     CDS             complement(21749..22246)
                     /gene="ubiC"
                     /locus_tag="KJL99_002466"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HBD4437159.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(22469..>22852)
                     /locus_tag="KJL99_002467"
     CDS             complement(22469..>22852)
                     /locus_tag="KJL99_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4437160.1"
                     /translation="MIKANAWYLYKEWIRSDDVSPIFIETEEKLRTFNTNKLTRNDNI
                     FILFSSVDDGPVMVVSSQRLHDMLNPTKDTNWNSTCIYKSRHKMLPINLTQETLFSSK
                     SHGKYALFPIFTASWRATRIKNKGI"
BASE COUNT         5879 a         5940 c         5495 g         5538 t
ORIGIN      
        1 cctccataac ggaggcttct tttttatggc tggcagatgc tttaatcatc caccttaaaa
       61 caatataacc tattgtttta atgacaaatc agaacggaat gtcatcatca aagtccatcg
      121 gcggctcgtt atacggcgct gccggagcgg actgctgcgg gcgagactgc gcgccgccgc
      181 tgaactgatt gccaccctgc ggctgctgag gctgacccca accgccctgc ggctgaccac
      241 caccgatatt gccacctgcc ggagcgccac caccctgacg accacccagc atctgcatgg
      301 tgccgccaac gttcaccacg acttctgtgg tgtagcgatc ctgaccggat tgatcggtcc
      361 atttacgggt acgcagctga ccttcgatat aaacctgaga acctttacgc agatattcgc
      421 tcgccacttc tgccagtttg ccgaacagca caacgcggtg ccattcagtc tgttctttca
      481 tctcgccggt cgctttatca cgccaggatt cggaagtagc cagcgtaatg ttggcaactg
      541 cgccaccatt tggcatgtag cgtacttccg ggtcctgacc cagattacca acgagaataa
      601 ccttgtttac gcctctgctg gccatgttcg tgtctcctga aaaaaatcgt tctgaataag
      661 tgtaaacgcg cgattgtacc attaccaata gcgcttttac tatgttgtga cctcggttcc
      721 gggaaacaaa cctggccaga cattgttaca caacactccg gataatgcat tccaatactg
      781 tatattcatt caggtcaatt tgtgtcataa ttaaccgttt gtgatcgccg gtagcaccat
      841 gccaccgggc aaaaaagcgt ttaatccggg aaaggtgaat ggataagatc gaagttcggg
      901 gcgcccgcac ccataatctc aaaaacatca acctcgttat cccccgcgac aagctcattg
      961 tcgtgaccgg gctttcgggt tctggcaaat cctcgctcgc tttcgacacc ttatatgccg
     1021 aagggcagcg ccgttacgtt gaatcccttt ccgcctacgc gcggcagttt ctgtcactga
     1081 tggaaaagcc ggacgtcgat catattgagg ggctttctcc tgccatctca attgagcaga
     1141 aatcgacgtc tcataacccg cgttctacgg tggggacaat caccgaaatc cacgactatt
     1201 tgcgtttgtt gttcgcccgc gtcggcgaac cgcgctgtcc ggaccacgac gtcccgctgg
     1261 cggcgcaaac cgtcagccag atggtggata acgtgctgtc gcagccggaa ggcaagcgtc
     1321 tgatgctgct cgcgccaatc attaaagagc gcaaaggcga acacaccaaa acgctggaga
     1381 acctggcaag ccagggttac atccgtgctc gtattgatgg cgaagtctgc gatctttccg
     1441 atccgccgaa actggaactg caaaagaaac ataccattga agtggtggtt gatcgcttca
     1501 aggtgcgtga cgatcttacc caacgtcttg cggagtcgtt tgaaaccgcg ctggaacttt
     1561 ccggtggtac agcggtagtg gcggatatgg acgacccgaa agcggaagag ctgctgttct
     1621 ccgccaactt cgcctgccca atttgcggct acagtatgcg tgaactggag ccgcgactgt
     1681 tttcgtttaa caacccggca ggtgcctgcc cgacctgtga cggccttggc gtacagcaat
     1741 atttcgatcc tgaccgcgtg atccaaaacc ccgagctgtc actggctggc ggtgcgatcc
     1801 gtggctggga tcgccgcaac ttctattact tccagatgct gaaatcgctg gcagatcact
     1861 ataagttcga cgtcgaagcg ccgtggggca gcctgagcgc gaacgtgcat aaagtggtgt
     1921 tgtacggttc tggcaaagaa aacattgaat tcaaatacat gaacgatcgt ggcgatacct
     1981 ccatccgtcg tcatccgttc gaaggcgtgc tgcacaatat ggagcgccgt tataaagaga
     2041 cagaatccag tgcggtacgt gaagaattag ccaagtttat cagcaatcgc ccatgcgcca
     2101 gctgcgaagg gacgcgtctg cgtcgggaag cgcgccacgt ttatgtcgag aatacgccgc
     2161 tgcctgctat ctccgacatg agcatcggtc atgcgatgga attcttcaac aatctcaaac
     2221 tcgctggtca gcgggcgaag attgcggaaa aaattcttaa agagatcggc gatcgcctga
     2281 aattcctcgt aaacgtcggc ctgaattacc tgacactttc ccgctcggca gaaacacttt
     2341 ccggcggtga agcccagcgt atccgtctgg cgagccagat tggtgcgggc ctggttggcg
     2401 ttatgtacgt gctggacgag ccgtctatcg gcctgcacca gcgcgataac gagcgcctgt
     2461 tgggtacgct tatccatctg cgcgatctcg gtaataccgt gattgtggtg gagcatgacg
     2521 aagacgcaat tcgcgccgct gaccatgtga tcgatatcgg tcctggtgcg ggtgttcacg
     2581 gcggtgaagt ggtcgcagaa ggtccgctgg aagcgattat ggcggtgcct gaatcgttga
     2641 ccgggcagta catgagcggt aaacgcaaga ttgaagtgcc gaagaaacgc gttccggcga
     2701 atccagaaaa agtgctgaag ctgacaggcg cacgcggcaa caacctgaag gacgtgacgc
     2761 tcacgctgcc agtcggtctg tttacctgca tcacaggggt ttcaggttcc ggtaaatcga
     2821 cgctgattaa cgacacactg ttcccgattg cccaacgcca gttgaatggt gcgaccatcg
     2881 ccgaaccggc accgtatcgc gatattcagg gactggagca tttcgataaa gtgatcgata
     2941 tcgaccaaag cccaattggt cgtactccac gttctaaccc ggcgacctat accggcgtgt
     3001 ttacgcctgt gcgcgaactg tttgcgggcg taccggaatc ccgtgcgcgt ggttatacgc
     3061 caggacgttt cagctttaac gtccgtggcg ggcgctgcga agcctgtcag ggcgacggtg
     3121 tgatcaaagt ggagatgcac ttcctgccgg atatctacgt gccgtgcgat cagtgcaaag
     3181 gtaaacgcta taaccgtgaa acgctggaaa ttaagtacaa aggcaaaacc atccacgaag
     3241 tgctggatat gaccatcgaa gaggcgcgtg agttctttga tgcggtgcca gctctggcgc
     3301 gtaagctgca aacgttgatg gacgttggtc tgacgtacat tcgcctgggg cagtccgcaa
     3361 ccacactttc tggtggtgaa gcccagcgcg tgaagctggc gcgtgagctg tcaaaacgcg
     3421 gcaccgggca gacgctgtat attctcgacg agccgaccac cggtctgcac ttcgccgata
     3481 ttcagcaact cctcgacgtg ctgcataaac tgcgcgatca gggcaatacc attgtggtga
     3541 ttgagcacaa tctcgacgtg atcaaaaccg ctgactggat tgtcgacctg ggaccagaag
     3601 gcggcagtgg cggcggcgaa atcctcgtct ccggtacgcc agaaaccgtc gcggagtgcg
     3661 aagcttcgca tacggcacgc ttcctcaagc cgatgctgta atcgttaagg ccgctttctg
     3721 agcggccttt tcctttcaga gttgtaccag caatttacgc ttttcttccg gtagtaaatt
     3781 cactgcctgc tgataagacg catccaccag ataatagatt tgcgaatccg gcagcgaacc
     3841 gtcgagatac acggtgctcc agtgcgcttt attcagatgg cggctaggac gcacatcgct
     3901 gtgctgctga cgtagcagct ccgccagctc cgggctggtt ttcagcgaaa cagctgggcg
     3961 attttctact tctttcacca tcgcaaacag tacatcttcc actttgatct gcgtcgcttt
     4021 ccagtcgtta tgcacgctct gttctgcgct tggttttgcc atgcaatatt gtagcaactc
     4081 cgaaatggtc attttttact ccccttgtag tgtcgcgatg atgcgacgcg atccgccgtg
     4141 gatgcgatgt tcccccagcc agatgccttg ccaagtgccg gtctgaatac gccctttatg
     4201 taccggcaat acaagcgatg ttcccagcat tgaggatttg atatgagaag gcatatcgtc
     4261 tgctccctca tagtcatgct cataatttcc gttgtcggga acggtgcgga ggaaaaaaca
     4321 ctccatgtcg tggcgtacgg tgggatcgca gttctcatta agtgtcagag aggcggaggt
     4381 atgttgcagc aacagatgca gtaagccgat gttaacgcgc ggcatatcag ccagctgatt
     4441 cagaatttca tccgttacca gatgaaaccc acgagatttg gcgctaagcg tgagcgtctt
     4501 ttgataccac atgtgctgct ccttgataaa actctcttaa tcagtttgca gcaagacagc
     4561 gaaaggataa aggtgtgatt aaaaaaacag cattcgggag agcatcactc tctcccgctc
     4621 tgtcagtatt ctgaattgac gatcacctct tcaccaaacg cacccgcttg tggcaagggt
     4681 ttgtaggtag agttggaggc gcgcagaatg cggataccac gagcgccgac atcgcgtgcg
     4741 gcggtaatat cgttatcgga atcgccataa aaaattcgga tatttttatc ctgcagccat
     4801 tgcgattttg tattttgccc tggtttatcg cccgcaaaga ttaccggatt catgttggtg
     4861 gcaggaatat gaaaattatc cgccagcgtt tttgaaaccg tttctgtttt cgtcgggcta
     4921 cgaccagtca caaagaagat cgcgtcaccg cggcgtacat gcatatcaat cagctggcga
     4981 gcgacctctt ttggaatgct gaattcatcc cagccattat tcattttttc ccagaacaca
     5041 ggatttttca gataatcttc gctttctggc gagaaggttt ttttgccgcg ccagaacccc
     5101 ggactggaaa aaagtaccgt gtcatcgata tcaaacccca ccgccattgg cggacgccct
     5161 gcgaggctat tttcaatttg tgcgaccgaa acccaatgaa ttggtgcctg ttcagcaagc
     5221 ctggcaacgt tagtcccagg gttaagcggt gaaggagatg aggccagggc aacagcggaa
     5281 ctgtttagag cgaacaataa gcaaacggca ctgattgcct gtgtgatctt gcgcatattt
     5341 ttccctaaat agtcagtatg ttgaaacttt ttattgtgag atttgttgca aaaaccatct
     5401 gaccataacg ccagctgccg gggaaaggaa ggattatctg cggtttttgt gatgaaaagc
     5461 aggatgatta tatttaaccc agtaaatacg ttcctgtctt tttttgtata aaaaatgttt
     5521 attactcgat gtaaggatac attaatttta cgtttattat gatgaatcct cggcaaatga
     5581 gcgaaatttg atagaacatt tatctgctgt acgatttaat gaacttaatg atacagcatt
     5641 acaaaaacaa tcgaagttta taaagatgat ttctgattga ccagccccct accctacaat
     5701 ggtgattaat tttattactc gcgatggaga tgatgatatg aatatatcat atgtgaatag
     5761 caacaaaacg acatctttac ctgttgagct agatgcacta aataataaag atatctctta
     5821 tgccaaagat ttctttttat acatagaaac acagttaaaa attgcaaaag atttttgtag
     5881 acctggagaa gaagtatcaa gttctattgc aagtaaagtt tttcacgcat ttattgattt
     5941 agttaacaaa atcaggggta agaaagactt tatgtatatt tgcacgcttt gctgttttgc
     6001 cgaggaggtt aaaggtgatt attctcatta caggaccttt ttatttgata ttggtaatca
     6061 atataaggtt aaacttacac aaagcggaaa aaagagttct ctttaacatt agaatttaac
     6121 gatactataa ttgaatctca gaaagtcaca ggcaataagg caaagcatat tcttgaggat
     6181 atagaaatat tctatcgtaa caaacccgat acttattatt aaatttaata atggttagat
     6241 gtgcagaaat tattactcca cacgaaaaaa tagggaaagg aattccagat aattgggata
     6301 aacttaacaa taaaagaact gacaacgttt tattatctct cacgtagaac aacggcatca
     6361 aaaaaatcat ctacaaagaa atagtgaaag cgacgacagt ttaatttcac tgcaggctgg
     6421 gtaactccag cctgctttcc tgcattacat caccgcagca aacgcctttg ccacacgttg
     6481 tacatttgcc gtatttaacc cggcgacaca catgcgaccg ctggcgatga gatagacacc
     6541 aaattcttca cgtagtcggt caacctgagc ggcacttaaa ccggtataac tgaacatgcc
     6601 gcgctgatta agcagataat cgaaattgcg ttctggcatc tctgtgctta ataccttcac
     6661 caattcctga cgcattgcca gaatgcgagt acgcatctct tctacttccg ccagccagct
     6721 ggctttcaat gcctcgtcat tcagcactgc agccaccacc tgcgcaccaa aattcggcgg
     6781 gctggagtag ttgcggcgaa ctgttgcttt caattgcccc agtacgcggc cagcggcttc
     6841 ggcatcttca cacagaacag aaagtccgcc gacgcgctcg ccgtaaaggg agaaaatttt
     6901 cgagaacgaa ttgctcacca gagcgggtaa tccagcactg gcaatggcgc gaatagcgta
     6961 ggcatcctct tccataccgg cactaaatcc ttgataggca atatcgagga atgggataag
     7021 ctcgcgggct ttgagaattt caatcaccgc atcccactga tcattagtga gatcggcacc
     7081 cgttgggttg tggcaacatg gatgcagcaa cacaatactg cgggcaggta atgttttcag
     7141 cgtcgccaac aggtcattaa agcgcacgct gttagtcact tcgtcatacc aggggtaagt
     7201 actcacttcg aatccagccc cggcgaatat tgctacgtgg ttttcccaag taggatcgct
     7261 gacccagacg cctgattccg ggaagtagcg tttcaggaaa tccgcgccca ctttcaatgc
     7321 ccctgagccg ccaagggttt gaatggttgc tacgcgctgt tgttgcagta ccggatggtc
     7381 cgcaccaaac agcagcggcg caatggcatg gcgatagctg ttaagccctt ccatcggtaa
     7441 ataaagcgaa gcgccatggg gctgcgcatt caggcgcgct tccgcctccg ccacggcttt
     7501 caattgtgga ataattccgt cttcgttgta gtacagaccg atacttaaat tcactttgtc
     7561 gctgcgaggg tcttctttaa aacgctccat aagcgtaaga atcgggtcgc cagcgtaggc
     7621 gtcaactttt tgaaacacgc gatggttctc caggtttacg ggcaggtggt taaaacacaa
     7681 taaaccggaa gaaggcgaag atcgagtgga tgttcaggag cgaacggcaa ttagcaacag
     7741 agtgagacta atgataaacg tacatccgcc agaggcacga cctttataag agcatgaaag
     7801 aacatcaact tattgaattt ttaggatttt attggccgga taaggcattc acgccgcatc
     7861 cggcacagac aatcaaatat tacagaacga ttaatccacg tatttcatcg cgacccttga
     7921 agtcaggcgc gtaataagtt cgtaagcgct tactttcgtc atttcagcga tacgttctac
     7981 gggcaaacct tcgccccata aaatgaccgg atccccggct ttgtcctgcg cctgtggacc
     8041 taagtctacg cagatcatat ccatcgccac gcgcccgaca atcggtactt cgcgaccgtt
     8101 caccagcact ggcgtaccgg acggcgcggc gcgcggataa ccatcgccat agcccatcgc
     8161 gactacgcca agacgggtat cacgttcgct tacccaggtt ccaccataac caacaggctc
     8221 tccggcttta tgctcacgca cggcaatcag gctggaggtt agtgacatca ctggctgaca
     8281 gccaaaatcg gcaccggtgg agcgatcttc cagcggcgag acgccataaa gaatgatgcc
     8341 cgggcgcacc cagtcaaaat gcgactgtgg ccacagcaga atgccacccg acgcggcaat
     8401 ggaacgttga ccaggtttgc cttcgcaaaa ggtattaaag atagcgagtt gtttctcggt
     8461 tgcgccacat tttggttcat ccgcgcgcgc aaaatggctg acgatattca ccggctgacg
     8521 aacgtttttg cactgggtca ggcgatgata aaacgcctca gcctgttccg gccttacgcc
     8581 cagacggtgc ataccggtat cgagtttcat ccagacggta accggctcgt ccaagctagc
     8641 ctcttccagc gcagccagct gttcttcgtt atgcacggcg gtatgaaaat gttgcgcaga
     8701 aatcgtcggc agatctctgg catcaaaaaa gccttcgagt aacagtacag gtttggtgat
     8761 tccccccgca cgcagtcgca gagcttcttc gagacgggct acgccaaagg cgtcagcatc
     8821 ggggagcgtt cgcgcggtct caagaagacc gtgaccataa gcgttcgctt tcaccaccgc
     8881 aaccatttta ctggcaggtg ccagttcacg aagacgttgc aggttgtgtc gcagagcgcg
     8941 gcggttaatc acaacagttg ccgcttgcat ttgtgttcct tgataattgt ttgctttaat
     9001 tacctaattc ataaaataat tattattcgt cgtcgtactg cggccccgca tagttgtcga
     9061 agcgcgacca ttgaccgtta aaggtcaggc gtaccgtccc gattgggccg ttacgttgtt
     9121 taccgataat aatttccgcg atgcctttta aatcactgtt ttcgtgatac acctcatcac
     9181 gatagataaa catgatcaag tccgcatcct gctcgataga gccagattca cgcaggtcgg
     9241 agttgaccgg acgtttgtcg gcacgttgtt ccagagaacg gttcaactgg gacagcgcca
     9301 ccaccggcac gttcagttct ttcgccagtg ctttcagcga gcgggagatt tctgcaattt
     9361 ccagcgtacg gttatcggaa agcgccggta cgcgcatcag ttgcaggtag tcgatcatga
     9421 taagcccgat gccgccgtgt tcacgggcaa tacggcgtgc gcgggaacgc acttccgttg
     9481 gcgtcaggcc ggaagagtca tcgatataga tattgcgttt ttcgagcaaa atacccatgg
     9541 tgccggaaat gcgcgcccag tcttcatcat cgagctgccc ggtacggatt ttagtctggt
     9601 caacgcgcga cagcgacgcc agagaacgca tcatgatctg ttctgatggc atctccagcg
     9661 agaagataag tactggttta tcctgcaaca ttgccgcgtt ttcgacgagg ttcatcgcaa
     9721 atgttgtttt acccatcgac ggacgcgcgg cgacgatgat caaatccgac ggctgcaagc
     9781 cagcggtttt tttgttgaga tcgtcataac cggtgtttac cccggtaacg ccatcgtgtg
     9841 gctgctgaaa caactgctca atacgcgcca cggttgcgtc gagcacatcg gcgatgttct
     9901 tcggcccttc gtctttgttt gcacgacttt cggcaatttt aaagacgcgg gattcagcaa
     9961 ggtccagcag atcttcgctg gtacgcccct gcggatcaaa accggcttca gcaatctcat
    10021 tcgcaaccga gatcatctca cggacaacgg cacgttcacg cacgatgtcc gcataagcac
    10081 tgatgttcgc cgcacttggc gtattttttg acagctctgc cagataagca aaaccaccga
    10141 cgctatcgag ttgcccctgg cgttccaacg attccgcaag ggtaatcaga tcgataggac
    10201 taccgctttc ctgcaaacgc gccatttcag taaagatatg acggtgtggg cgggtgtaaa
    10261 aatcgtctgc taccacacgc tcggctacat catcccagcg ttcgttatcc agcattaaac
    10321 cgcccaacac cgactgctcc gcttcgatcg agtgcggagg cactttcagc ccggcaactt
    10381 gtggatcgcg ttcgcggggt tcagcctgct gtttgttgaa gggtttattt cctgccatag
    10441 tgaatggagt taccgaaata aagaatgggt cgaaacttta ccatatgaag cagaccctga
    10501 cgatacgttc tggaggacac atggcaacac gaattgaatt tcacaagcac ggtggcccgg
    10561 aagtacttca agccgtagag ttcactcctg ccgatccggc ggagaatgaa atccaggtcg
    10621 aaaataaagc catcggcatc aattttatcg acacgtatat ccgcagcggc ctttacccgc
    10681 cgccatcgct acccagcgga ttaggtaccg aagcggcagg catcgtgagt aaagtcggca
    10741 gtggtgtaaa gcatattaag gctggcgatc atgtagtcta tgcgcagtcg gcgttaggcg
    10801 cttacagctc tgtgcataac attaatgcgg ataaagcggc gattctgcct gcggcaattt
    10861 cttttgagca agctgcggca tccttcctga aaggcttaac ggtttattat ctgctgcgca
    10921 aaacctatga aattaaaccc gatgaacagt tcctgttcca cgcagcggct ggcggcattg
    10981 gcttaattgc ctgccagtgg gcaaaagccc tgggcgcgaa actcatcggc accgtaggaa
    11041 ccgcgcaaaa agcgcagagc gcgctaaaag ccggcgcgtg gcaggttatt aactatcgtg
    11101 aagagaatct ggtcgagcga ttgaaagaga tcaccggcgg taagaaagtg cgcgtagtgt
    11161 acgattccgt gggcagagac acctgggaac ggtcgctgga ttgcctgcaa cgccgcggct
    11221 taatggtcag ttttggcaac tcatcaggtg cggttaccgg tgtgaactta ggcattctca
    11281 atcaaaaagg ctcgttgtat gtgacacgcc cttccctgca aggctatatc accacgcggg
    11341 aggaattaac cgaggccagt aatgaactgt tctctttgat tgccagcggt gtgattaagg
    11401 tcgatgtcgc cgagcagcag aaatatccgc tgaaggatgc gcagcgtgcg catgagattc
    11461 tggaaagccg ggcgacgcaa ggttccagcc tgctgattcc ataaaagaaa tagggcttcc
    11521 acctgggaag ccctttcttt ttatagttcg gctgtatgta gggtacagca cgatgaatct
    11581 gttagaggcg caatagtgac agatttgatt atcaattcct attttgttct aaggataaaa
    11641 ccttaggttg tgatcatccg cacaatccct tagtaacgcc agcggtcata acgctgatat
    11701 ttcggcattt ttggtgcttt aatcgcctta ataacccaca ccaccgcaat cgccagtagt
    11761 aaccacggca gcaacttaat catcaatgcc agcataccgc cgaggaacat aatggccgtc
    11821 gccacaacca gcgcggcgat aatgcccagc aacgaaacgc cggtgaccat cagcatgaca
    11881 aaaaagccaa tcacaaaaag tagttccagc atgatgctct cccaaatatg aaatctcttg
    11941 ctggcattac aagaatcatg ccaaaaataa tctattgatt taacagcaaa acgccccgcg
    12001 acggtgcgca gggcgtggtg aatttgacta ctttttggtg aaaagttaac gcttatccgc
    12061 caccagtttg agcgcgtgtt ccagcacgtt aatgtcagca cccgctttat gggcattttc
    12121 acttaaataa cgccgccact gccgcgcgcc aggaataccc tggaacaaac ccagcatatg
    12181 ccggataata tggccgagat acgtcccctg gctgagttca tgctcaatgt acggatacat
    12241 ggcacgcact actgccaccg gatcggcatc ggtatccgag gaaccaaaga tctcccggtc
    12301 taccgccgcc agaatacccg gattctgata cgcctcgcgc ccgaccatca cgccatccat
    12361 atgttgcaaa tgcgctttag cttcttccag cgacttgata ccaccgttaa tcgacattgt
    12421 cagatgcggg aagtcacgct tcagttgata cacacgcgga taatcgagcg gcgggatttc
    12481 acggttttct ttcgggctta accccgaaag ccaggcttta cgtgcgtgga tgatgaacat
    12541 ctcacactcg cctttgccgg aaacggtatt gatgaaatcg cagagaaatt catagctgtc
    12601 ctggtcgtcg atgccaatac gcgttttcac cgtcaccgga atcgacacca catcgcgcat
    12661 cgctttcacg cagtcggcaa ccagctgcgc attacccatc agacacgcac caaacatgcc
    12721 gttctgcacc cggtcagacg ggcagccgac attcaggttg atctcatcat atccgcgcgc
    12781 ttctgccagc tttgcacact gcgccagcgc cgccggatcg ctaccgccca gttgcaacgc
    12841 taccggatgt tcttcttcac tgtacgccag ataatcacct ttaccgtgaa ttatcgcccc
    12901 tgtggtcacc atttcggtat aaagcaacgt attgcgggaa agcagacgca agaaatagcg
    12961 gcaatgtctg tccgtccagt cgagcattgg tgcaacacta aaccgaccac tccagtgaac
    13021 gtcagttttt tcaggcattg cgctggtttg gttgattttt tgcatttcag aattaccgtg
    13081 cattttcaaa tgtagagatt attttatcga tatatcatgg ggttatgtta ttcagcttca
    13141 ctgttcagga ggctcaatag cggggtacta taccataaca acaggaagcg cctgtctcat
    13201 tgcaaaagaa aattgagatc ctctcaaggc atgaagctct cacgaagtga tggaaataat
    13261 cttattagcc gttaactttg ttaaggccaa tgataaacaa tccaggttcg acgataaata
    13321 aaaaatcaca cattaaactc tggtgatata tctccctgct aatagcattg atagagaaaa
    13381 aagaacccaa taaatattgg gtccttttat ataatgcctt ccatactatc gaagaacttc
    13441 acatattatt tctccgattt aaccccgaac aagtcataaa ttaatttaga agtgtctgta
    13501 agtattttaa tctcttcctt tgaggttggg tcaaacgact tcgcgaagct aattaattgt
    13561 ggtgcagcat ctcgcatttt gcttaaaata tcaggatcga gcgttccttc attcaccaga
    13621 tgtgacatct tatccagata gccatcaaaa ttcatttctc ccccatctgc cagacgcttc
    13681 atctctccca ggtacttctt catatcacgt tgatataatt tttcaaactg agctttcagg
    13741 tagctctcat tattttcatt aatatacact gtttctgata aatctgcgta ggcactagaa
    13801 tatgaaagtg aaattaacag cgaagcaaat aattttaagc tgttgtcatc atgttcctgg
    13861 caggcattaa caaacgttaa aaaaccagaa cctattgctt gaaaatgtat attcgctaaa
    13921 ggttcattat ccttagattc ctcataaaat agctgaaccg tggagggaag gccattctta
    13981 gtttctacat caaaagtaca ttgttcaatg ggcattgcat ctttattatc cccaggatta
    14041 catatactat taatcgcgaa atgaaaaata gcccggtcaa caactgatgc aataaagtca
    14101 cgatcgttta aatcctcatc agtgcaatcg tcagtatact tattgaccca cattttgaga
    14161 tcatttatgt gctcaattaa aaacacattt ttttacgtgc atgccataaa ttatgcgtga
    14221 ttcgttgaat aaacaaatct atttttgcaa taattatatc ccgatgacgc agcgctgcat
    14281 aatcaataga tttaccatat atattattta atttaccttt gattgaacct ctattatcca
    14341 atttatcttt ggttgcatct ttattttcga attgatttga attgagtgtt ttattaaagt
    14401 atccagttgc accgggtatt atcttcaaca tgattcatct accttttttg tcgtaccgtg
    14461 tcctccttat aattcttttg ccatctggtc actatcaaaa atcgctcata atttctgaac
    14521 tacaggtatg cgtttaaacc cagttaaata aatagcgagt taacttgaag caatttattt
    14581 aaaaatgaat tattaattat tatcgtgttg ataatggtac gataaggcaa cacaacacct
    14641 tacctacaaa acagaaaatc aacattacgg caacaataag ggttctcggt gtttttcctg
    14701 gaacatggta aagtaaggac attcttaacc cccactttga ggtgcccgat ggaaaagacc
    14761 acaacgcagg agttattagc gcaggctgaa aaaatctgcg cgcagcgtaa tgtgcgcctg
    14821 accccacagc gcctggaagt gttgcgcctg atgagtctgc aggatggcgc tatcagcgct
    14881 tatgatctgc ttgatttact gcgcgaagct gaaccgcaag ccaagccgcc aacggtttat
    14941 cgcgcgctgg attttctgct tgagcaaggt tttgtgcata aggtggaatc caccaacagt
    15001 tatgtgctct gtcatctgtt cgaccagccc acgcatacgt cagccatgtt tatttgcgat
    15061 cgctgcggcg cggtgaaaga agagtgtgca gaaggcgtgg aagacattat gcatacgctg
    15121 gcggcaaaaa tggggtttgc cctgcggcat aatgtgattg aagcacatgg gctctgtgcg
    15181 gcatgcgtgg aagtggaagc gtgtcgtcat cctgaacagt gccagcatga tcactctgtg
    15241 caggtgaaaa agaaaccgcg ttaagagggc gtacatcctt gtacacgtcg ggcaggaggg
    15301 attaattacc agcgatattc attgcgggtt tcccaatcca cgacctcttt ttctgcctga
    15361 tctttctgat aaccataacg ttcctggatt ttaccgacca gttgatcacg tttaccttca
    15421 atgatcgtca tatcatcatc ggtcagtttg ccccattgct ctttcacttt acctttaaac
    15481 tgtttccagt taccgccggc ttcatcttta ttcataatca agacctcatc gttaggttgt
    15541 gaatgagagt acgttcactt ttcttctgaa cgtgagatta agtgtagtca gcaatttggc
    15601 ttaggataat ttattcagaa gttttaaccg tcacgttgcg gcaaaccagg taccgttgcg
    15661 ccagtgacgc cgccagatag ccgccagaga aagcccgcgc aacgccagaa agacggttaa
    15721 tgccagccac aaaccatgat tacccagcca cggcagcgta aggagcgtca gcgcaaaacc
    15781 tgcggcggcc accgccatac tgttacgcat ttcggcggca cgcgttgcgc ctataaacat
    15841 gccgtccagc agataacacc agacgccaac caacggcaaa atcacctgcc agataagata
    15901 gcggtcagcc agctgctgaa tctgggttaa cgacgtcagc aacgcaatga tgtgttcccc
    15961 tgccagcaaa taaaccaccg aaaatagtaa cgctacaatc cccgactggc ggcacgctgc
    16021 ccgccataca tccagcaact ggctaccgtc gcgcgcaccg tatgcctgac cggagtgcgc
    16081 ttcaaccgcg taggcaaaac catcaagcgc ataggcggta aaggtgagta gcgtcatcag
    16141 aaccgcgtta acagcgataa tgtcactccc cagtcgcgca ccaagtacgg tgatcgcgcc
    16201 gaaacagagt tgcaacaaca gcgaacgcag catgatatcg cggttaagcg ccagcaagcg
    16261 acggaagttt cctcgccagg cagttttcag catttcgccg gaaattccgc gtagtttgag
    16321 gattttacgc accattagca gaccaatcag caatgttgca tattccgcaa taaccgtcgc
    16381 cagcgccgcg ccctgcacgt tcatatgcag ccccatcacc agccagacat ccagcacaat
    16441 gttgaggata ttgccgacca ctaacaaaat tactggcgca cgggcatatt gcacgccgag
    16501 taaccaaccg agtaatacca gattcgccag cgacgccgga gcgcttaacc agcggatttc
    16561 aagaaagcgc cgcgcctgtt ccaggaccgc ttcactaccg ccaacaatat gcagcgccag
    16621 atcgataatc ggcgtacgca acagcgcaat taacgccccg gcccccaacg ccaacagcaa
    16681 cggttgcacc agcgcacggg ctaatgcctg aggatttttg gcaccataag cctgcgcagt
    16741 cagcccggtg gtgctcatgc gtaaaaacag caacagcata aagagaaagc tggtcgccgt
    16801 tgcaccaacc gccacgccgc ccaaataaac cggactatca agatgaccaa ttaccgccgt
    16861 atcgaccagt cccagcaacg gaacggtgat attggagaaa atcatgggta aggcgagatg
    16921 ccagagtgct ttatcagatg aagtgaggaa tgccatgcag acaagcctga tgaagagaga
    16981 tgaaaaacaa accgcgacgc caagcggcat cgcggtctca gagatatgtt acagccagtc
    17041 gccgttgcga ataaccccaa ccgccagccc ttcaatggtg aagctctgct gacgaaggtc
    17101 aacgacaatt ggtttaaact cgctattttc tggcaacagt tcgactttat tgccctgttt
    17161 tttcaggcgc ttaacggtaa cttcgtcatc aatacgtgcg acaacgacct gaccgttacg
    17221 tacatcctga gttttatgca ctgccagcaa gtcgccatcc ataatgccga tatctttcat
    17281 cgacatcccg ctgacgcgca gcaggaaatc agcattcggc ttgaacaagg aaggatcgac
    17341 ctgataatga ccttcaatat gctgttgcgc cagaagcggt tcaccggcag ccacacgacc
    17401 taccagcggc aacccttctt cctcttcctg caacagacga atcccgcgtg atgcgccgga
    17461 aacaatttca ataacgcctt tgcgtgccag cgccttcaga tgttcttcag ccgcgtttgg
    17521 ggaacggaac cccaaacgct gcgcgatttc cgcacgcgtc ggcggcatac ctgtctggct
    17581 gatgtgatca cggatgagat caaacacctc ttgttgcctg gccgttaacg ctttcattcc
    17641 gccccctggg tgtatataca gttatactgt gagtatatac agcaaaaggc gattttggaa
    17701 ccataaactg cacaataaac cagagattta tcgaaatctg gaagggttat ccaaaatgcg
    17761 accataacag gatgcaccag gtaatcacgg cgacgataat ggcaatcagc accgcggcgg
    17821 atcccatatc ttttgcgcgt ccggaaagct catggtattc agagccaatt cggtcaacca
    17881 ctgcttcgat ggcgctattg aggatttcca caatcatcac cagcatcacg gagctgataa
    17941 gcagcacgcg ggtaattgcg tccacatcca gccagcaggc gatgaccacc gccaacaata
    18001 ccgctacgcc ttcctgacgg aatgccgctt cgttgatcca tgcagcgcgt aaacctttcc
    18061 aggaatagcc agcagctttg ataattcggg tgaatccagt tgtattattg gccattgaaa
    18121 gaaccttttt acattatgag cgtcaatatc agtgtaccgt taaatccgat gattcgggtt
    18181 tgcgtaacgc cgcgcgaaac atgagcggat accacagaat ttcccatgac tttctgctat
    18241 ccttgccgcg catttgcatt attaaccaga ggctttacat cgtttatgtc cggctggcca
    18301 cgaatttact acaaattact gaatttacca ttaagcatcc tggtaaaaag caagtctatt
    18361 ccggcagatc ctgccccgga actggggctg gatacctctc gtccaattat gtacgtttta
    18421 ccgtacaact cgaaagcaga tttgctgacg ttgcgcgccc agtgtctggc acatgatttg
    18481 cctgacccgt tagagccgct ggaaatcgac ggcacgctac tgccgcgcta tgtgttcatt
    18541 cacggcgggc cgcgtgtatt cacctattac acgccgaaag aagagtctat taagcttttc
    18601 cacgactatc tcgatttgca ccgtagcaac ccaaatctgg atgtgcagat ggtgccagta
    18661 tcggtgatgt ttggtcgcgc gccggggcgt gaaaaaggcg aagtgaaccc gccgctgcgt
    18721 atgcttaacg gcgtacagaa atttttcgct gtactgtggc tcggtcgcga cagttttgtg
    18781 cgtttctcgc cgtcagtgtc gctgcgccgt atggcggatg aacacggcac ggataaaact
    18841 atcgctcaga aactggcgcg cgtggcgcgt atgcactttg cccgtcaacg tctggctgcc
    18901 gtaggcccac gtctgcctgc tcgtcaggat ctgtttaata agctgctcgc ctcccgcgcc
    18961 attgccaaag cggtagaaga tgaagcgcgc agcaaaaaaa tctcccatga aaaagcgcag
    19021 cagaacgcga ttgcgctgat ggaagagatt gcggcgaatt tctcttacga gatgatccgc
    19081 ctgactgacc gcattctggg cttcacctgg aaccgatttt atcagggcat caacgtccat
    19141 aacgccgagc gcgttcgcca gctggcccac gacggtcatg agctggtgta tgtgccttgc
    19201 caccgcagtc acatggacta cctgctactt tcttacgtgc tgtaccacca ggggctggtg
    19261 ccgccgcata tcgccgccgg gatcaacctg aacttctggc cggccgggcc gattttccgc
    19321 cgtctggggg cgttctttat tcgccgtact tttaaaggca ataaacttta ttccaccgtt
    19381 ttccgtgagt atctcggcga actgttcagc cgtggttatt ccgtcgagta cttcgtggaa
    19441 ggcggtcgtt cccgcacggg gcgtttgctg gatccgaaaa ccggtacgct gtcgatgacc
    19501 attcaggcga tgctgcgtgg cggcacgcgt ccgattacgc tgattccgat ctatatcggt
    19561 tatgagcacg tcatggaagt gggtacttac gccaaagaac tgcgcggcgc gacgaaagag
    19621 aaagagagcc tgccgcagat gctgcgcggt ttaagcaagc tgcgtaatct cggtcagggt
    19681 tacgtcaact tcggtgaacc aatgccgttg atgacctacc ttaaccagca cgtaccagac
    19741 tggcgtgaat ctatcgatcc catcgaagcg gtgcgtccgg cctggttaac gccgacggtc
    19801 aataatattg ctgccgatct gatggtacgc attaacaacg caggcgcggc aaacgccatg
    19861 aacctgtgct gtactgcgct actggcatca cgtcagcgct cactcacccg cgagcagtta
    19921 accgagcaac tcaactgcta cctggatctg atgcgcaacg taccttactc cacggactct
    19981 accgttcctt cagccagcgc cagcgagctt atcgatcacg cgctgcaaat gaacaagttt
    20041 gaagtcgaga aagacactat cggcgacatc atcattctgc cacgcgagca agcggtgctg
    20101 atgacctact atcgcaacaa cattgcgcat atgttggtgc tgccttcgct gatggcggca
    20161 attgtcaccc agcatcgcca catctcccgc gacgtattga tggagcacgt caatgtgctt
    20221 tacccaatgc tgaaagcgga gctgttcctg cgctgggatc gcgacgagtt gccggacgtt
    20281 attgatgcgc tggcaaatga gatgcaacgt caggggctga ttaccctgca agatgatgag
    20341 ttgcatatca acccggcgca ttctcgcacc ctacagctgc tggccgcagg cgcgcgcgaa
    20401 acgctgcaac gttatgccat caccttctgg ttgttgagtg ccaacccgtc gatcaaccgc
    20461 ggtacgctgg agaaagagag ccgcaccgtt gcgcaacgtc tctccgtgct gcacggcatc
    20521 aacgcgccgg agttcttcga caaggcggtg ttcagttctc tggtgctgac gctgcgtgat
    20581 gaagggtata tcagcgatag cggcgatgcc gaaccagcag aaacgatgaa ggtttatcag
    20641 ttgctggcgg agttgattac atcagacgtg cgtttgacga ttgagagtgc gacgcagggc
    20701 gaagggtaat cagagagaat tgccggatgc ggcgaaaacg ccttatccgg cctaccatga
    20761 cctgcaaatt caataaattg cgattcaccg ggtaggcctg agaagcgcaa cgcatcaggc
    20821 aacccagaag aaagccggat gatcatccgg cttttttaca tcatcagaaa tgccagtaac
    20881 tcattgccag tcctaaaaac agtaccagac caacatagtt attattcata aatgctttaa
    20941 agcaggcttc acgctcgcgg ttggcaatca gtttttgttg ataaacaaac agcgcgccag
    21001 ccaccacaat tgaccagtaa tatccccagc ctaagccatt taactcaccg atgatcgcca
    21061 tcagtgccag tacgccaatc tgcaaaatac caataatcaa tttatcgtat tggccgaaca
    21121 ggattgccgt ggatttaatg ccaatcttca catcatcatc gcggtcaacc atcgcatact
    21181 gcgtgtcgta agccaccgcc cagagaatat tggcgaggaa cattaaccag caactcaatg
    21241 gcaccgactc actcacagcg gcaaaagcca ttggaatcga ccagccaaac gccgcgccca
    21301 gcaccacttg cgggagatgg gtataccgct tcataaacgg gtacacccac gccaatgcca
    21361 gagcggcaat cgacaacaga atggtcatcg tattcagcgt cagaaccagt aaaaacgaaa
    21421 tcagtaccag cactacaaac agcgcgcgcg cctctttctc tgttaccgcg ccgctgggaa
    21481 gtggtcggtt cgccgtgcgc ttaacatgac catcaaactt gcgatcagca taatcattca
    21541 ccacgcaacc agcagcgcgc atcagccaga cacccgcgac aaacaccgcc atgatccaga
    21601 gctgaggaac gcccggtgtc gccacccaca acgcccataa tgttggccag agcagcagta
    21661 acgcgccaat tggcttatcc gtacgcatta agcgatgaaa cgccagcagc ttattctgcg
    21721 tcagactcca ctccatattt ttttcctctt agtacaacgg tgacgccggt aaaaacagtt
    21781 ctgttagcaa cagcggttta ccgcttaatc gcaggcggga acgtcgcccc cacagtccgg
    21841 catcacggcc tatctcgata aaatcccggg ttagtgtcga tgatgtgaac agatagcgtc
    21901 ctaacggcgt tttacccaat ttttgtaacg ctagttccgg cccgcttaat gttgatacag
    21961 gaacgacggt acgaccggca agccacggtt caccatcggc acataacaaa atttcacgta
    22021 accagtaacg agactctttc ggcagcagcg gcagttcttc ggggatttca ttctgctcga
    22081 caaacccctc gcggatcatc gtcacactca ccgtttttcc ctgctgttca aaacgttttg
    22141 tcatggaatc ctccagcaac agccagtcga gcagttgcgg atccagggca gggatctctt
    22201 taaaatagcg cagcgcacgc agttgcgtta acgcggggtg tgacatgccg aactctccgt
    22261 tacataacgt aaaggcattg tatcgcagaa aagtgtttac gggagatgta aagcaaatta
    22321 agtgatctaa tgcgcaacaa aaacgcaaca atcgcatgaa gtggtaaaac gctgttgaac
    22381 cttacgcttt gccgatgccc gtgctggcga gggcaaccat aggggcatca ccgcccatga
    22441 tgctaggaac ggtgatgttt tcctttactt aaataccttt attctttata cgagtagctc
    22501 gccaactcgc agtaaaaatt gggaaaagcg catatttacc atgagatttg gagctgaaaa
    22561 gtgtttcctg agtaagatta ataggcaaca tcttatgtct ggatttatag atacacgtgg
    22621 aattccaatt ggtatctttt gtaggattca acatatcatg taagcgctgg gagcttacca
    22681 ccataacagg cccatcatcg acagaggaga acaggataaa tatattatcg tttcgtgtta
    22741 atttattagt gttaaaggtt cttaactttt cttcagtttc tataaatata ggtgaaacat
    22801 catcactacg aatccactct ttataaagat accaggcatt tgctttgatc at
//
LOCUS       DAEBXN010000061        22416 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.061, whole genome shotgun sequence.
ACCESSION   DAEBXN010000061 DAEBXN010000000
VERSION     DAEBXN010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22416
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.061"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            231..1664
                     /gene="cscA"
                     /locus_tag="KJL99_002468"
     CDS             231..1664
                     /gene="cscA"
                     /locus_tag="KJL99_002468"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HBD4437161.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALALGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWAFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRRISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLILWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1672..2730)
                     /locus_tag="KJL99_002469"
     CDS             complement(1672..2730)
                     /locus_tag="KJL99_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBD4437162.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            2898..3116
                     /locus_tag="KJL99_002470"
                     /pseudo
     CDS             2898..3116
                     /locus_tag="KJL99_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3134..4462
                     /gene="dsdA"
                     /locus_tag="KJL99_002471"
     CDS             3134..4462
                     /gene="dsdA"
                     /locus_tag="KJL99_002471"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HBD4437163.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPSTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASCFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="KJL99_002472"
     CDS             complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="KJL99_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HBD4437164.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPRLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQKTMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="KJL99_002473"
     CDS             complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="KJL99_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HBD4437165.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLVLKRTDIRSPVTGY
                     IAQRSVQVGETVSSGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7687..8301
                     /gene="evgA"
                     /locus_tag="KJL99_002474"
     CDS             7687..8301
                     /gene="evgA"
                     /locus_tag="KJL99_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HBD4437166.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8306..11899
                     /gene="evgS"
                     /locus_tag="KJL99_002475"
     CDS             8306..11899
                     /gene="evgS"
                     /locus_tag="KJL99_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HBD4437167.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKIKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPASDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="KJL99_002476"
     CDS             complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="KJL99_002476"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HBD4437168.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLYNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     LGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="KJL99_002477"
     CDS             complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="KJL99_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HBD4437169.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="KJL99_002478"
     CDS             complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="KJL99_002478"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HBD4437170.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPIIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15936..17186)
                     /gene="frc"
                     /locus_tag="KJL99_002479"
     CDS             complement(15936..17186)
                     /gene="frc"
                     /locus_tag="KJL99_002479"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HBD4437171.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17699..18322)
                     /locus_tag="KJL99_002480"
     CDS             complement(17699..18322)
                     /locus_tag="KJL99_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HBD4437172.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18619..18894
                     /locus_tag="KJL99_002481"
     CDS             18619..18894
                     /locus_tag="KJL99_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4437173.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDVPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(18971..19213)
                     /locus_tag="KJL99_002482"
     CDS             complement(18971..19213)
                     /locus_tag="KJL99_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HBD4437174.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19566..20486
                     /gene="lpxP"
                     /locus_tag="KJL99_002483"
     CDS             19566..20486
                     /gene="lpxP"
                     /locus_tag="KJL99_002483"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HBD4437175.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20841..20912
                     /gene="ypdK"
                     /locus_tag="KJL99_002484"
     CDS             20841..20912
                     /gene="ypdK"
                     /locus_tag="KJL99_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HBD4437176.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(20977..22215)
                     /gene="alaC"
                     /locus_tag="KJL99_002485"
     CDS             complement(20977..22215)
                     /gene="alaC"
                     /locus_tag="KJL99_002485"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HBD4437177.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKCYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENHDRIRQAIRGIKA
                     MFRADGLLPVSSKHIHENAE"
BASE COUNT         6347 a         5208 c         4866 g         5995 t
ORIGIN      
        1 tactttggct gacatttttt tcccttaaat tcatctgagt tacgcatagt gataaacctc
       61 tttctcgcaa aatcgtcatg gatttactaa aacatgcata ttcgatcaca aaacgccata
      121 gttaacgtta acatttgcga tattcatcgc atttatgaaa gtaagggact atatttttat
      181 aaaagttaac gttaataatt caccaaattt gcttaaccag gatgattaaa atgacgcaat
      241 ctcgattgca tgcggcgcaa aacgcactag caaaacttca tgagcaccgg ggtaacactt
      301 tctatcccca ttttcacctc gcgcctcctg ccggatggat gaacgatcca aacggcctga
      361 tctggtttaa cgatcgttat cacgcgtttt atcaacatca cccgatgagc gaacactggg
      421 gaccaatgca ctggggacat gccaccagtg acgatatgat ccactggcag catgagccta
      481 ttgcgctagc gctaggagac gataatgaca aagacgggtg tttttcaggt agtgctgtcg
      541 atgacaatgg tgtcctctca cttatctaca ccggacacgt ctggctcgat ggtgcaggta
      601 atgacgatgc aattcgcgaa gtacaatgtc tggctaccag tcgggatggt attcatttcg
      661 agaaacaggg tgtgatcctc actccaccag aaggaatcat gcacttccgc gatcctaaag
      721 tgtggcgtga agccgacaca tggtggatgg tagtcggggc gaaagatcca ggcaacacgg
      781 ggcagatcct gctttatcgc ggcagttcat tgcgtgaatg ggccttcgat cgcgtactgg
      841 cccacgctga tgcgggtgaa agctatatgt gggaatgtcc ggactttttc agccttggcg
      901 atcagcatta tctgatgttt tccccgcagg gaatgaatgc cgagggatac agttaccgaa
      961 atcgctttca aagtggcgta atacccggaa tgtggtcgcc aggacgactt tttgcacaat
     1021 ccgggcattt tactgaactt gataacgggc atgactttta tgcaccacaa agctttttag
     1081 cgaaggatgg tcggcgtatt gttatcggct ggatggatat gtgggaatcg ccaatgccct
     1141 caaaacgtga aggatgggca ggctgcatga cgctggcgcg cgagctatca gagagcaatg
     1201 gcaaacttct acaacgcccg gttcacgaag ctgagtcgtt acgccagcag catcaatctg
     1261 tctctccccg cagaatcagc aataaatatg ttttgcagga aaacgcgcaa gcagttgaga
     1321 ttcagttgca gtgggcgctg aagaacagtg atgccgaaca ttacggatta cagctcggca
     1381 ctggaatgcg gctgtatatt gataaccaat ctgagcgact tattttgtgg cggtattacc
     1441 cacacgagaa tttagacggc taccgtagta ttcccctccc gcagcgtgac acgctcgccc
     1501 taaggatatt tatcgataca tcatccgtgg aagtatttat taacgacggg gaagcggtga
     1561 tgagtagtcg aatctatccg cagccagaag aacgggaact gtcgctttat gcctcccacg
     1621 gagtggctgt gctgcaacat ggagcactct ggctactggg ttaacataat atcaggtgga
     1681 acaacggatc aacagcgggc aagggatccg cgtcactctt cccccttcac gaccttcaat
     1741 aatatgcaat gcagcttccc gcccgataat gtcatgtgga agctgaattg tggtcagcgg
     1801 cggtaaaaac agatgcccga cgccaaccag attatcaaag cccattacag cgacatcctg
     1861 cgggatacgt acccccttcg ccaaaagaac ctgataagcc acaaaggctg cgcgatcgtt
     1921 accacatatc agaacatcaa aatctggttt gcccgatttg aagtgagcat tgagtaaact
     1981 tgcgagatcg gtgtagtgat catcacctgt tgccatgtga aattgtttca cctcagccag
     2041 atctcgtcca gcatcacgcc aagcctgctc aaatccctgc cgacgatacc ctgttgccaa
     2101 cgcactttcc ggtagccaga agcataatgg ttgacgatag cccgccgcga gcaaatgctg
     2161 tgttgattca tattgtgcag tgtaatcatc agggatataa ctgggtaacg ctgggtcatc
     2221 cgccacacag ttcgccaata caatattttc accatacaga gactcaggca gcgtgatatg
     2281 tcgcagcccc attgtagtat agataatgcc atccggacgg tgggcaagca gctgacgtgc
     2341 cgcgcgggca gcgtcatctt cagaaaaaat attgattaaa aaactattcc agccgaactc
     2401 gctggcggtt tgctcaatgg caagcagaat atcaacagag aaaggagtgg tagccgtgtc
     2461 ctgcgccagc acggcgagag tcgacggctt acgtccttga gcgcgcatct tacgggcgga
     2521 aagatcagga acataattca gggtctggat tgcctgcaat acgcggtcac gcgttgcagg
     2581 acgcacagat tctgcattat gcatcacccg ggagactgtc atcatcgaca ctcccgccag
     2641 gcgtgcgaca tcctttaatg aagccatacc caagccgttt gccgtaaaac gggcactgta
     2701 gcagaaacag acgtcactgg cgagatccaa cgccctatca cctgacacag caatacaata
     2761 aaaaataaca ataattcccg gacaattgtc cccaattccg cctctgttct cgccaacgag
     2821 taaccatata ggcaagtaag taaaacatgc caactggcat cgccaacctg gagaagaaaa
     2881 tataaatatg gatcattgtg ctgccactgt atcccgacat cagcccggaa attattgcga
     2941 ttgctatcgg ttcaggtgca attggctgca cgatcgttac ggactcgctt ttctggctgg
     3001 tgaagcaata ttgcggcgct acgctcaatg aaacatttaa atactatacg acagcgacat
     3061 ttatcgcttc agtcgtcgct ctggcgggca cattcctgct gtcatttatc atctaagcgc
     3121 aaagagacgt actatggaaa acgctaaaat gaactcgctc atcgcccagt atccgttggt
     3181 aaaggatctg gttgctctta aagaaaccac ctggtttaat cctagcacga cctcattggc
     3241 tgaaggttta ccttatgttg gcctgaccga acaggatgtt caggacgccc atgcgcgctt
     3301 atcccgtttt gcaccctatc tggcaaaagc atttgctgaa acagctgcca ctggggggat
     3361 tattgaatca gaactggttg ccattccggc tatgcaaaaa cggctggaaa aggaatatca
     3421 acaaccgatc agcgggcaac tcctactgaa aaaagatagc catttgccca tttccggctc
     3481 tataaaagca cgcggcggga tttatgaagt cctggcacat gcagaaaaac tggctctgga
     3541 agcggggttg ctgacgcttg atgatgacta cagcaaactg ctttctccgg agtttaaaca
     3601 gttctttagc caatacagca ttgctgtggg ctcaaccgga aatctggggt tatcaatcgg
     3661 cattatgagc gcccgcattg gctttaaggt gacagttcat atgtctgctg atgcccgggc
     3721 atggaaaaaa gcgaaactgc gcagccatgg cgttacggtc gtggaatatg agcaagatta
     3781 tggtgttgcc gtcgaggaag gacgtaaagc agcgcagtct gacccgaact gtttctttat
     3841 tgatgacgaa aattcccgca cgttgttcct tgggtattcc gttgctggcc agcgtcttaa
     3901 agcgcaattt gcccagcaag gccgtatcgt cgatgctgat aaccctctgt ttgtctatct
     3961 gccgtgtggt gttggcggtg gtcctggtgg cgtcgcattc gggcttaaac tggcgtttgg
     4021 cgatcatgtt cactgctttt ttgccgaacc aacgcactcc ccttgtatgt tgttaggcgt
     4081 ccatacagga ttacacgatc agatttctgt tcaggatatt ggtatcgaca accttaccgc
     4141 agcggatggc cttgcagttg gtcgcgcatc atgctttgtc gggcgggcaa tggagcgtct
     4201 gctggatggc ttctataccc ttagcgatca aaccatgtat gacatgcttg gctggctggc
     4261 gcaggaagaa ggtattcgtc ttgaaccttc agcactggcg ggtatggccg gacctcagcg
     4321 cgtgtgtgca tcagtaagtt accaacagat gcacggtttc agcgcagaac aattgcgtaa
     4381 tgccactcat ctggtgtggg cgacgggagg tggaatggtg ccggaagaag agatgaatca
     4441 atatctggca aaaggccgtt aataacgttt caacgcaaca tcgcaatcct ttccctgggt
     4501 gagcgatgct gccgatggcg cagacttaag atccccggtc ttacccgcta taaccccctc
     4561 cttttatcat cacccaacgc ctttcgctgt aaacggcggt ttcgcaaacc aaaccaaaac
     4621 cgtaagtaag ataaaagcaa tagccgccat acggaaaatc tcatttgcag aaatagaaag
     4681 tgactgctgg gttatttcat tattaatttc attaagaact cctgaaagcg aaccatagta
     4741 tttatccata atttgtgacg atgaattaaa cacggggtta aattgatcga tggttgctgt
     4801 caactgacta tggtgtaacg attcgcgtcg tccccacagc gtcattgtca acgacgtacc
     4861 aactgatcct gacaaggtac gaaaaaaatt actcatactc gaggcattgg caaatttatt
     4921 atctggcaag cctgaaaacg aaatcgttgt taagggtaaa aagaaacagg caacggcgaa
     4981 tccctggaaa aactgtggca aaatgatgcc tgtaaaatca atcgttggca taaatgtcac
     5041 agaacgccag taatagcaaa ccgcatacat caaaaaactg aatgtcacta acacccgcat
     5101 gtctatttta ttgccataac gtcctatcaa aggtgaaatc aatagtggca tgatgccgat
     5161 gggcgcataa gcaagcccgg cccatatcgc attatacccc atcgttttct ggagtaactg
     5221 cggcataagg acgatcgctc cagagtaaaa taaatacgcg caggtgatac tcacaatacc
     5281 aatggtgaaa ttacgggact taaacaaact gagatcaaga atcgggttct ctgaggtcga
     5341 ttcccaaatg actaaagaga tcagagaaat aactgatact actgttaata ttattattgt
     5401 actcgagttg aaccaatcca gatcgcgccc tttatcaagc ataatttgca agccaccaac
     5461 accgagcact aacagggtca gtcttggtag attcattttt accggtgaag tttcagtttc
     5521 tcttccttta agtaaggtta agcataatgt caggacgata atccccatag ggacattgat
     5581 taaaaatatc caaccccagc taaagttatc gcaaatatag ccgcccaata tcggcccaca
     5641 tatcggagcg ataatcacgg tcattgacca taatgccaga gcaaatgttc ttttttctgg
     5701 tggataattc cttaataata aactctgtga cagtggaatt aacggccccg ccattaatcc
     5761 ctgaacgact ctaaaaaata tcagcacatc aagattggtc gataaactac acattaagga
     5821 agacagcgaa aaaaaagtga ctgaaagtaa aaataacctt aattcgccta ttctttgtgc
     5881 cagcctgccg gtaacaggga tcgcaatggc atttgctacg ccaaacgagg tgataaccca
     5941 ggtgccttcg tctgttgatg ctcccagaaa gccagatatt gtcggtattg cgacgttaga
     6001 aatagtggaa tccaacattt gcataaacgt cgctaatgac aatgcaatag taacgcacca
     6061 taacgtccca ccggttaatg gtgccggagt tgatttagtg attgccatta aagttgtcca
     6121 ttatgcgaaa taatgttgct aatttctttt tctatcggac tggtatcgat aactaaagcc
     6181 ttactggtat aagcaggcat ggaggtcacg gttgaagcca gatcaggcat ctcagcaatg
     6241 tcttcgtcct tcgtatcaat agttgcagtc atcgataaac caatgcgcaa ggggtgttcc
     6301 atgagttctt ttggatcaag agaaacttca accggtacac gctgaacgat tttgatccag
     6361 ttccctgtcg cattttgtgc aggtaataag gagaacgcat tgccggttcc catattgatc
     6421 cctgtcaccc gaccatgaaa cacaacattt tcaccataaa gatcgctgat aatattgact
     6481 gattgaccaa tccgtacatc cgtgagttgt gtttctttaa agttggcatt aacccacatt
     6541 tgacgtgccg gtacgacagc cattaacgat tgtccggaac tcactgtttc gccgacctga
     6601 acacttcgct gggcaatata gccagtaacc ggactcctta tatccgtacg tttaagcacc
     6661 aaccaggctt ctttggttgc atccgccgct tcaactactt gtggctgacg gtttaatggt
     6721 gtgttcatta ctaaagcttt attcgcttta taagcctgga tagcggcatt cagtgccgct
     6781 ttgctactta ttaacgtatc tttggtatgc tccagcgttt cttttgaaat aaccccctgc
     6841 ttcgctaaag gcacacgacg gttataatct tctaaagatt gttgatactg aatacgtgct
     6901 gaagcgactt cggcactgta ttgtttatcc tgtaagtaca gtttattcgt ttgccgaaca
     6961 atatttgcca gattattttt agctttattg agtgcgatag tggcatcagt tttatccagt
     7021 gaaactaaaa tgtcaccctg tcgaacgtag ttcgtatctt tatgattaac tacagtgaca
     7081 ctacctgaga cttgtgcaga aattggatct gcattccccg tgacataggc gtcatctgta
     7141 ctaatcatgt cttctaattc cattgaccaa taggcatagg cacctgaaaa cgcaataaat
     7201 aaaactatca ccaataaaga aaagtatttt tttctgttag actgtttttt atttgaatta
     7261 atctgttcca ctattatctc tcatttcgca tagatgaaat ttatgaattg aatgaaaaga
     7321 gtattcattc ttcatatcaa acacagtgca tgtgttgcac attatcccag gaacgttgga
     7381 ggcctaattt aaatgaggtg caaaaaaaac aacagaggac taaaccgtgg cttttgcaat
     7441 acaattctta cgcctgtagg attagtgaga agacttatag tgctaacttg aaactataaa
     7501 tcatcggtat aatccctgat tttattgttg acatttcatt tatgccgact atttatatgg
     7561 tatacttgtc gaattatctt aaaggaaact cagattttct tatttttatt gagaaaatga
     7621 gatgacgcct tatgtctgta ttactacagg gagaagggag atgcttcatt gcaaagggaa
     7681 taatctatga acgcaataat tattgatgac catcctcttg ctatcgcagc aattcgtaat
     7741 ttattgatca aaaacgatat tgaaatctta gcggagttaa ctgaaggcgg aagcgccgtt
     7801 cagcgggtgg aaacacttaa gcctgatatc gtcatcattg atgtcgatat ccccggagtt
     7861 aacggtatcc aggtgttaga aacgctgagg aagcgccaat atagcggaat tattattatc
     7921 gtctccgcta aaaatgacca tttttacggg aaacattgtg ctgatgctgg cgctaatggt
     7981 ttcgtgagta aaaaagaagg catgaacaat atcattgcgg ctattgaagc tgcaaaaaat
     8041 ggctactgct atttcccctt ctctctcaac cggtttgttg gaagtttaac gtccgaccag
     8101 caaaaactcg actccttatc gaaacaagaa attagtgtca tgcggtatat tcttgatggc
     8161 aaggataata atgacattgc ggaaaaaatg ttcatcagca acaaaactgt cagcacgtat
     8221 aaaagtcgcc tgatggaaaa attagaatgt aaatcactga tggatcttta cacattcgcg
     8281 caacgtaaca aaatcggcta atcacatgaa gtttttaccc tatttttttc ttctctgttg
     8341 tggtctttgg tcgaccataa gtttcgcaga cgaagattac atcgaatatc gtggcatcag
     8401 tagtaacaac cgtgtcacac ttgatccagt acgtctgagc aacaaggaat tacgttggtt
     8461 agcgagcaaa aaaaatcttg tgattgcagt acacaagtcc caaacggcta ccttgttgca
     8521 taccgattcg cagcaacagg ttcgtggtat taatgctgat tatttaaatc ttttaaaaag
     8581 agcgttaaat atcaaattaa cactccggga atacgcagat catcaaaaag caatggacgc
     8641 gcttgcagaa ggtgaagtcg atatagtgtt atcacattta gttacttcgc cgcctcttaa
     8701 taatgacatt gctgcaacca aaccattgat aattaccttt ccggcgctgg taaccaccct
     8761 tcacgactca atgcgaccgc ttacctcacc aaaaccagta aatattgctc gggtagcaaa
     8821 ttacccccca gacgaggtaa ttcatcaatc atttccaaaa gcaacaatta tctcttttac
     8881 aaatttatat caggcattag catccgtctc agctgggcat aatgattact ttattggtag
     8941 taacatcatt accagcagta tgatttcccg ctatttcact cactccttaa atgtagtgaa
     9001 atattataac tcgccgcgtc aatataattt tttcttgacc agaaaagaat ctgtcattct
     9061 taatgaagta ctcaatagat ttgttgatgc tttaacaaat gaagttcgct atgaagtatc
     9121 acaaaattgg cttgatacag gaaacctggc ctttctgaac aaaccattag aactcactga
     9181 acatgaaaaa cagtggatta agcagcatcc caatttaaag gtgctggaaa atccttactc
     9241 gcccccctat tctatgacgg atgaaaatgg ctcggttcgg ggcgttatgg gggacattct
     9301 taatattatt accttgcaaa caggtttaaa tttttctccg atcaccgttt cacacaatat
     9361 ccatgctgga acacaactta gccccggagg atgggatata atacctggcg ctatttatag
     9421 tgaagatcga gaaaataatg ttttatttgc tgaagccttc ataacaacgc cttacgtttt
     9481 tgtcatgcaa aaagcgcctg acagtgaaca aacattaaaa aaaggaatga aagttgccat
     9541 tccatattat tatgagctgc attcgcaatt aaaagagatg tatccggagg ttgaatggat
     9601 acaggtcgat aatgccagcg ctgcatttca caagattaag gaaggtgaac ttgatgctct
     9661 ggtcgcgaca cagctaaatt cgcgttacat gatcgatcat tactatccta atgaacttta
     9721 tcattttctt attcctggcg ttccgaatgc atcgctttcg ttcgcttttc ctcgcggaga
     9781 accggaactt aaggatatta ttaataaagc actgaatgca attcccccaa gcgaagttct
     9841 gcgcctgacg gaaaaatgga ttaaaatgcc caatgtgacc attgacacat gggacctata
     9901 tagcgagcaa ttttatattg ttacgacatt atccgtttta ttagttggca gtagcctttt
     9961 atggggattc tacctgttac gctcagttcg tcgtcgtaaa gtcattcagg gtgatttaga
    10021 aaaccaaata tcattccgaa aagcactctc ggattcctta ccgaatccaa cttatgttgt
    10081 aaactggcaa ggtaatgtca ttagtcataa tagtgctttt gaacattatt tcactgcgga
    10141 ttactacaaa aatgcaatgt taccattaga aaacagtgat tcacccttta aagatgtttt
    10201 ttctaatgcg catgaagtca cagcagaaac gaaagaaaat cgaacaatat acacacaggt
    10261 atttgaaatt gataatggca tcgagaaaag atgcattaat cactggcata cattatgcaa
    10321 tcttcctgca agtgacaatg cagtatatat ttgtggttgg caagatatta ctgaaacgcg
    10381 tgatctaatt aatgcactcg aggtagaaaa aaataaagcg ataaaggcta ccgtagcaaa
    10441 aagtcagttt ctggcaacga tgagtcacga aataagaaca ccaataagct ctattatggg
    10501 cttcctggaa cttctgtcgg gttctggtct tagcaaggag caacgggtgg aggcgatttc
    10561 acttgcctac gccaccggac aatcactcct cggcttaatt ggtgaaatcc ttgatgtcga
    10621 caaaattgaa tcgggtaact atcaacttca accacaatgg gtcgatatcc ctactttagt
    10681 ccagaacact tgtcactctt tcggtgcgat tgctgcaagc aaatcgatcg cattaagttg
    10741 cagcagtaca tttcctgatc attacctggt taagatcgac cctcaggcgt ttaagcaggt
    10801 cttatcaaat ttactgagta atgctctcaa atttaccacc gagggggcag taaaaattac
    10861 gacctccctg ggtcacattg atgacaacca cgctgttatc aaaatgacga ttatggattc
    10921 tggaagtgga ttatcgcagg aagaacaaca acaactgttt aagcgctaca gccaaacaag
    10981 tgcaggtcgt cagcaaacag gttctggttt aggcttaatg atctgcaaag aattaattaa
    11041 aaatatgcag ggcgatttat cattagaaag tcatccaggc ataggaacaa catttacgat
    11101 cacaatcccg gtagaaatta gccagcaagt ggcgactgtc gaggcaaaag cagaacaacc
    11161 catcacacta cctgaaaagt tgagcatatt aatcgcggat gatcatccga ccaacaggct
    11221 attactcaaa cgccagctaa atctattagg atatgatgtt gatgaagcca ctgatggtgt
    11281 gcaagcgcta cacaaagtca gtatgcaaca ttatgatctg cttattactg acgttaatat
    11341 gccgaatatg gatggttttg agttgactcg caaactccgt gagcaaaatt cttccttacc
    11401 catctggggg cttacagcca acgcacaggc taacgaacgt gaaaaagggt taagttgcgg
    11461 catgaactta tgtttgttca aaccgttgac cctggatgta ctgaaaacac atttaagtca
    11521 gttacaccag gttgcgcata ttgcacctca gtatcgccac cttgatatcg aagccctgaa
    11581 aaataatacg gcgaatgatc tacaactgat gcaggagatt ctcatgactt tccagcatga
    11641 aacgcataaa gatctacccg ctgcgtttca tgcactagaa gctggcgata acagaacttt
    11701 ccatcagtgt attcatcgca tccacggtgc ggctaacatc ctgaatttgc aaaagttgat
    11761 taatattagc catcagttag aaataacacc tgcttcagat gacagtaagc ctgaaattct
    11821 tcagctgctg aactctgtta aagaacacat tgcagagctg gaccaggaga ttgctgtttt
    11881 ctgtcagaaa aatgactaaa ttgcggctcc cacaatgttc taatgtggga gctatttacc
    11941 agcacatctt acatttatga tgagaactcc tggcgaattt gttccccatg ctggtcgagc
    12001 gttgccgctc ccggcataac atgcgggtcc gcgcagccgc tgatttttat cggattaccc
    12061 ggcatcatta ttcctccggc ttcaatcaac atatttctcg cctgagtttg tggcaaatta
    12121 atggcctcag ccacacttaa taacggcgcg acgggcacac caacttcatg tattttggct
    12181 aaccaaactt cagctgcctg cgtttttaac gttcgctcaa tatattgttt aagaatagcc
    12241 tggttttgta cgcgtaaaat attgctgcta aatcggggat cattaaccag ttccgtaagc
    12301 tccagtgcct ggcataacgc agaaaaaagc ttgtcattac cacaacaaat cgtaatcggc
    12361 ttatcctgag tatcgaaaac atcaaaaggt gccatgtagg gatggcgatt tcccaggcgt
    12421 tgtggtgact tccctgtcgc aatatatgcc atcagaccat gctccagaaa actcagcgtg
    12481 gcatcaaaca tcgctatatc gacatgcgcc cctctctggc tcttttcgcg gccataaagt
    12541 gcactcacta ttccgctgaa taaatagaca ccgccgcata gatccgcaag agaggtacca
    12601 acacgcactg gcggagcatc agggtaccct gtttccatca ttatcccact cattgcctga
    12661 atgatggtat cgtaggcagg agcatctttt agcggaccgg tatgtccgaa acctgacgat
    12721 gaagcatata tgaggcgcgg gttgatttct tgtaaccttt cccatgaaaa ccccagtttt
    12781 tccattgtac ctaggcgaaa attctcagct aatacatcag cttgtttgag catatttata
    12841 aatatacttt tatcgtgatc attctttaaa tcaagaacca cactctcttt gccatgatta
    12901 ataaaactgt aatagagtga ctgtccatcc acatagggac caaatgtgcg ggtatcatca
    12961 ccatgacctg gtggctcaac tttaattacc cttgcgccca tattataaag aagttgagtt
    13021 ccgaaaggtc cattaaggac atgtgtcata tcgataacta ataagccttc aaacggccct
    13081 ttgctttcat tatttgtcat ttgcttatgc cttgaaaaaa gccgaaggtg aaaagacact
    13141 tcggcattta tgtaaaatca ataacatgat taattatgaa accagtcgac tgacataaat
    13201 ccacaaggga gccgtgacga caaagcccag tacgctcaca gccaatgacg ctgtaccagt
    13261 acgggtgtag acattaaacc ggctggcaat aatgattccg gagaatgccg gcggtaatgc
    13321 gcctgccagt accatcatct gcaaatgttc gctgttcaaa tgacatgcca taccaacgag
    13381 aagcagcgcc agtggcatca gaatcaactt caggaaggtg ttataagcaa tttcggcact
    13441 gaactcgaat ttatgtgcag ccagagtcaa ccccgcagcg aataccgcta cccctgagtt
    13501 agccttcgca atcagattaa aggttgggtc ccatgctgcc ggaattttta ccccaaccag
    13561 caccaggatc gttgccagaa caggtgccca tactactggc tcctttgccg cagaaattaa
    13621 tgcgctcaga ttactattct tcttaccatc cgctcctgag gaaggattca gcaaatacag
    13681 accaatagga atagtaattg cgttaacaat aatagaaata attgctacca ctaaacctgt
    13741 tgatacggaa tcaccataaa taggatcgag aactgcaaac cccaggaatc caatggtagg
    13801 tgaacccgca attaatgcac atacagctgc ttctgcatgg gtacgtttaa aaaatttgta
    13861 gcaaccaaac caggagaaaa agaaacatcc gacaataacc acaagtgata caagggtcag
    13921 acgagtgtcc gcaaaaatca tttccctgtt tgcccgggta atagaaacaa atagagccgc
    13981 aggaagagcg tagtttaata ccagtttatt aaatgcgcgg gcttgatctt ctgaaaaagt
    14041 ttctcgtctg ccgctaaaat aacccaacag catgattacg ataataggca ataaatcgcc
    14101 aataaaaaat gttagcataa tagtatttcc gataagggtc ccctcaccat cgcataatga
    14161 gttggcaaga ggagacgatg tcagtattta attaccagcg acttgttttg ggttaagttt
    14221 ggtaatatgg ccactttcag ttcctgctgc agggtcgata accacattaa taatggtcgg
    14281 tttgcgcgac tgaataccgg tggttaaagc atgacgaagt tcatctgtcg tggtgacgtt
    14341 atagccaacg ccacgaaacg catccattaa tttgtcatac cttgcatggt gcaacagatc
    14401 cgttggtgat ggtgcaccag cgccactgag atcaacaccg tctcctctgt agatgccgcc
    14461 attattaaaa ataacgatcg tcaccggcag gttatatcga caaatcgttt caatttccat
    14521 cccactgaaa ccaaaagcac tatcaccttc aatggcgaca accggagaac cagaagtcac
    14581 gctagcaccg atggcatagc ccataccgat gcccatgaca ccccaggtac cacaatccag
    14641 acgacgacgt ggtttataca tatcaataat atttcgtgca ttatccaggg tatttgcacc
    14701 ttcattaact aaataaatat cctggttctc gcgcaatacg tcgcgcacag cacttaatgc
    14761 attaaagtaa tttaatggtt gagtatctgt acttaatttt tcatgcattt tttgtgcatt
    14821 ttgctgcttg tggatattta aaatatcgcg ccataccagt ggagtcgtaa atgtgttttg
    14881 tttcagttct gccagcatac cttgcatact ggatgcaata tcaccaacga ctggcacagc
    14941 aatggggcgg ttgctgtcaa tttcctgcgg ttcaatatcc agttgaataa actgtgtatc
    15001 tgccgcccat ccttttttac cgtgtgccaa taaccaattc agtcgtgcac caacaagcat
    15061 gacaacgtca gcatttgcca gggcaaacga acgcgcagct gccgcagaaa gtggatgcgt
    15121 atcttcaagg atccctttcg ccatagacat tggcaggaat ggaatctgag cactttcaat
    15181 aaattcacga agctgttcat cagcttgtga atacgccgcg cctttgccaa ggataattaa
    15241 tggccgttca gcttttgcta aaagcgaaat tgcgctagtg actgacttcg ggcatggcaa
    15301 taatgctggc gacggatttt caactttaac aatcgtggtt aacgcttcgt ctttttccat
    15361 cgtcgcggcc aggacatttg ctggcaaatc aagataaact ccgccagggc gacccgatac
    15421 agagacccgg atagcgcgtg ccaatgcaat gccaagatcc tgcggctgat taacgcgaaa
    15481 tgctgctttg gcatacggtt ttgccgcatt catttggtcc agctcttcat aatcaccttg
    15541 ctgtaggtcg acgatcgcgc ggtcgctgga gccgctaatc atgataatcg gaaaaccatt
    15601 taccgttgcg ttggccaatg cggtcaaacc attgaggaat cctggcgcag aaactgtcag
    15661 gcagatcccc ggtttttggg taagaaaacc gcttgccgca gcggcatagc ctgccgactg
    15721 ctcatgacga aaaccaatat aacgaatgcc ttccgcctgg gcatggcgtg ccatatccgt
    15781 cacaggaata cctacaacac cataaatagt gtcaatatta ttctgtttta atgcttcaac
    15841 gatgatatgc ataccatctg tcatttgaag ttgatctgac atgattaata acctcgaaat
    15901 ttttataaat tgggagccag aagcccccgt taatatcaga tggcgtggtt ttgcttcatt
    15961 gcagcaattt catcgtcgct ataacccagc tcctgcaata cagcagcggt atgttcacct
    16021 aatagcggcg cagctttaat atccggcgta aaggcagaga atttcattgg acaaccaacg
    16081 gtcagatatt ttccacgcaa cggttgttcc acttcaacaa cactgccact ttggcgcaaa
    16141 gagggatcaa gtgaaatttc tttcatactt aaaaccggtg cacaaggaat atcaaactga
    16201 gtcaaatagg ccaccgcttc atgtttatca atagtgacag tgtatttttc gatttcagca
    16261 aaaatatcga aaatatgtgg ctgtcgggca tgggctgtac tgtatgccgg atcggtaatc
    16321 cattctggtt tgccgatggc tttacaggtg ttttcccagt tttgctcctg aatagtgaaa
    16381 taaatatagg cgttaggatc ggtttcccag cctttacatt tcaggatcca gccaggctga
    16441 ccgccaccac ctgcattacc accgcgggga actgcatcac caaatgtacc attcggatac
    16501 tgcgggtatt cttccagata acccaattta tcgagacgct gctggtcacg taatttcacg
    16561 cggcaaaggt tcaatacggc atcctgcatt gacatggtga ctcgttgccc acgccccgtt
    16621 ttttcgcgat gcagcaaagc agcaagtaaa ccgatcagca aatgcattcc ggtgttgctg
    16681 tcacccaacg ctgcagcgct taccagcggc ggaccatccc aaaaacccgt agtggatgcc
    16741 gcgccacccg ctgcctgagc aacgttttca taggctttta cattcacata aggcgaacac
    16801 tcatcaaacc ctttgatcga accaaaaatc agacgtggat tgatttcttg aatatgctcc
    16861 caggtgaagc ccatgtgatc aatggcccct ggatgaaagt tctcgactaa gatatcagct
    16921 tcgcggatca gcttttccat tacctctttg ccttccgctg ttttggtatt taactcaata
    16981 gaacgtttgt tactgttaag catggtgaag taaagcgcat cgatatcagg aatatctcgc
    17041 agttggtgac gcgttacgtc accaacgccg ggacgttcaa ttttaataac gtcagcgcca
    17101 aaccaggcca gcatttgagt acaagatggg ccagattgca caccggtgaa atcgagaact
    17161 ttaattcctt gaagtggagt tgacatagca atacctatta gttaattaaa tatgtgttaa
    17221 tttagcgttt atttaaggca taacgccctc tataaataat ctgcaaaata tcttcgtagc
    17281 ggcactcgat atttttaaat tcctgtatca agtactgaaa attattttat cgccacttcc
    17341 aattcctttc tgcatggtaa gtagtgagga caaaaagaag aatatgataa atattaattc
    17401 gtggtaatca gagagagtcg cgacatttgt gatgttattc ttacaactgt aagaagcgcc
    17461 ggtgatttac aagtaagagt atgattacgc tttttaaagc gtaccataaa gcataacata
    17521 ataaatgatt tgtgatgaca cgggattatt gcggtttact ttgaggaaaa taaaatgcgt
    17581 gtttatagaa ccgcgagcgc caaataattt tatgaccaca acctgtcgta ttgtggtcat
    17641 aaaaaattgt cggctggagg gagttaaatc ctttacctca atatactgcc tgtcagcatt
    17701 agttcacaaa cagtgcttcg ctatcgacga tgattccatc ttccgcacct ttaagcgcca
    17761 ggtttgcctc gtagtattgc tttttatcaa gcagtttctg cgcatcagca agtgcattac
    17821 gagtctgttt aagcggcatc agatattggt tttccattac gccaacaccg gccaggcgaa
    17881 gctcttccat tgcgcccttt ttatcccctt tcgccatttt ttcattggca atttttatcg
    17941 cggcttcttt ttcgggtgtt gctacataac tctcagaaat tccgacagat gcattaatga
    18001 caatatattg gtcatcattg agattggtct ttttacctgg cttagcgaac tttgcccatt
    18061 cggtactgtc atctgacagc aaagcggaag cttcattggt cagttctttt gccttttccg
    18121 gatcgccatg aaacagagcc aggcgcgcta cctgcacgtc acgcatcgca tataaacctt
    18181 gttcagagat acgctctgct gctgccgttt tctgggtttg ctgagtttgc tgttgtgccg
    18241 ctaccggtgc attatcagca gcccatacag ttgaagatgc cagaattgct gtgaccatcg
    18301 tagccataat taaacgtttc atacttacct cattctttaa cagttttaga aaacgccgcc
    18361 atcatattgg gtgagcatga aatgagaaat cagagagcaa cggtcatttt gaaagggtta
    18421 tcttacaatt gtagttatta tcgcaaagcg gcatttagtc ttacagttgt aggaatatgc
    18481 ctcctgtcgc agagaactat tcttatatac cacaaagaga tgtcatttat tatattgatg
    18541 taaatattga ttaattatat gtaaatacag ccataacatc gtatggttct cagtgaacca
    18601 ttttcaggat aactttctat gaaagtaaac ttaatacttt tcagcttatt tttattggtc
    18661 tctattatgg catgcaatgt ttttgcattt tccatttctg gtggtgtaag tgagaggagc
    18721 tataaagaga ctgaaaaaac atcagcgatg acgaccacac actctacaaa acttcagcca
    18781 tcacaggcga ttttgtttaa gatgagagaa gatgtgccac cattaaacct cacagaagaa
    18841 atcacgccta cttatccgac aaaggcgaat tatcttattc atcctgtgcg atagcgtctg
    18901 tcacatggca ggtgaacaat catgtctgat gctgatgtga acacctgccc aaatgagcca
    18961 tcctgaccca ctatcgcctg ccatacccca ccataaagcg aatcatcaag ccacaagccg
    19021 gggcaagaaa cataaatatc cccgtcacca gttcattacc gccagttaac cacatggtga
    19081 agtaataaat gagtgcagcg aggatcacca tgccgaagaa agaagagacc gccgatacca
    19141 cgttcagtac ctgaccgatg tagccgctta cgcagacaat acacagggcg agaaacatcc
    19201 ataaataaat catagctcaa cctcctgccg cgctaaaaaa cctgtaactt cagtcacttt
    19261 aatagtaaga caatccttaa cctctggcaa tttttgctaa gcatcaatga aaacagcacg
    19321 ttaaatgcaa cgctattgcg tgcggaaaat tttttacttt gcacgattaa taatcagtag
    19381 ctgaaagcag tcagccagtg ataaagtaga gccaccgcat tcatgctcct cgtggttatg
    19441 tcctgactag tctttacact ctttacagga accattgtcg tacatgatgg cccaaccaat
    19501 tgagtgtacc gctgtcgaca tggaatcttc tgcgatacaa caattcgtat ctacagaagg
    19561 taactatgtt tccacaatgc aaattttccc gcgagtttct acatcctcgc tactggctca
    19621 catggtttgg gcttggtgta ctctggcttt gggtacagct tccttatcct gttctctgct
    19681 ttctcggcac gcgtattggc gcaatggcgc gaccattcct gaaacgtcgt gaatctatcg
    19741 cccgtaaaaa cctggaactt tgtttcccgc agcattctgc ggaagaacgc gagaagatga
    19801 ttgccgaaaa ctttcgttca ctcggcatgg cgctggtaga aaccggcatg gcatggttct
    19861 ggcccgacag tcgcgtacgt aaatggtttg atgttgaagg gttggataac cttaaacgcg
    19921 cacaaatgca aaatcgcggc gtaatggttg tcggcgtcca ttttatgtcg ctggaactgg
    19981 gcggccgcgt gatgggactg tgccaaccaa tgatggctac ctatcgtcca cataataatc
    20041 agctgatgga atgggtgcag acccgtgggc gcatgcgctc taacaaagcg atgatcggca
    20101 gaaataatct gcgcggcatt gtcggtgcac tgaagaaagg tgaagcggta tggtttgctc
    20161 ccgatcagga ttatggtcgt aaaggcagct ccttcgcgcc gttctttgcg gtggaaaatg
    20221 tcgccacaac caatggcacc tatgttctct cccgtctctc tggcgcagcc atgttgaccg
    20281 taacgatggt aagaaaagcg gattacagcg gatatcgttt gttcatcacc ccagagatgg
    20341 aaggctaccc gacagatgaa aatcaagccg ctgcttatat gaacaagatt atcgagaaag
    20401 agatcatgcg cgcaccggag cagtacctct ggatccaccg tcgctttaaa acgcgcccgg
    20461 tgggagaatc gtcgttgtac atttaacaaa ctgctcttac tctttccctc ctcaaatcgg
    20521 ccataataac ctcatgattt ttatagcttt tttcttaagt tttttccttc gctgaatatc
    20581 taattattac cgtcagaaat taaactgtcg ccgatatgag acacttgtaa gttactaaaa
    20641 gtaaataaga aaatgcctct tgtctcgttt cagttttagg cgtacattag cgccgtctcg
    20701 agcagtttag ctcagaattc tggggtgatg cccacggtaa aatacagggc ttttaaggtc
    20761 gctttgcccg gtgtcaactc actgtatcag gtgtaatgaa gtcattcagg cgtaacagta
    20821 attacgcgga gagatgtaaa gtgaaatatt tctttatggg catttctttt atggtcatcg
    20881 tttgggccgg tacttttgcc ctgatgatct aaaagcaaac tgtgcaatga aaaacaggag
    20941 ccatcaggct cctgtttgca tttctgacga cggtgtttat tccgcgtttt cgtgaatatg
    21001 tttgctgctg acgggtagta aaccgtcggc acggaacatc gctttaatcc ctctgatggc
    21061 ctgacgaata cggtcgtggt tttcaatcag tgcaaagcga acatgcgtat caccatagtc
    21121 gccaaagcca atccccggcg agacacaaac cttcgcttcg ttaaggagct tcttggcaaa
    21181 ttccagcgat cccatggccg catatggttc cgggattttc gcccagacat acatcgaagc
    21241 cttcggcatt tcgaccatcc agcccgcttc atgcagccct ttaaccaata catcacgacg
    21301 gcgtttgtac tgttcggcaa tgtcgcgcac gcactgttga tcgccctcga gtgccgcaat
    21361 cgccgccacc tgcaacggcg taaaagtgcc gtaatcgtga tagcttttga ttcgcgccag
    21421 ggcactgacc agcgttttgt tgccgaccat aaagccgata cgccagcccg ccatgttgta
    21481 gcttttcgac agcgtaaaga actcgaccgc cacatcgcgt gcacccggca cctgcataat
    21541 tgacggcgct ttccagccat cgtagacgat atcggcatag gccaggtcat ggaccaccag
    21601 cacatcgtag catttcgcca gcgccacaac cttttcgaag aactccagtt ccacgcattg
    21661 cgcggttggg ttcgacggga agccgaggat catcatcttc ggtttcggat aactttcacg
    21721 aatggcgcgc tccagttcgt tgaagaaatc gacaccttcc accagcggca ctgagcgtac
    21781 ctgcgccccg gcaatcaccg cgccataaat atggatcggg taacttggat tcggcaccag
    21841 caccgtgtca ccatgatcca gcgtcgccag catcagatgc gccaggccct ctttcgaacc
    21901 aatagtgacg atggcttctg attccgggtc gatttcaacg tcgtagcgat cctgatacca
    21961 gcgggaaatg gcgcgacgta accgcggaat gccgcgtgaa gtggagtaac catgcgtgtc
    22021 cgggcgctgg gccacagtac ataatttttc gacgatatgc ggcggagtcg caccgtccgg
    22081 gttacccatg ctgaaatcga taatatcttc gccgcgccga cgcgcagcca ttttcagttc
    22141 agcggtgatg ttaaaaacgt agggcgggag acgatcaatg cgcgtaaagc gacgttcagg
    22201 gcgagtgtca gccataattt cctcagatta acgttagcgc ccggaccgtc cgagcgacgc
    22261 tgccacaatg gtggctgttt tgaaaatagc ctgattaatt tctgtctgtc cagcgtttta
    22321 gaacattttt atcaatggat ttaaatgagg aactccactt caaaaacagg aaatttttct
    22381 acgctaacaa gaaaatcacc accaatttta ttaaca
//
LOCUS       DAEBXN010000062        22412 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.062, whole genome shotgun sequence.
ACCESSION   DAEBXN010000062 DAEBXN010000000
VERSION     DAEBXN010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22412
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.062"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            82..2682
                     /locus_tag="KJL99_002486"
     CDS             82..2682
                     /locus_tag="KJL99_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HBD4437178.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQVCVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQTTLQWGGP
                     WGTTWYGGGQYAEYYRAAMFGLGFNRGDFGAISFDATQAKSTLADQSEHKGQSYRFLY
                     AKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNY
                     SKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNY
                     ALSGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRF
                     GAIATLDGIQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINS
                     AITRMDAICR"
     gene            2673..3656
                     /locus_tag="KJL99_002487"
     CDS             2673..3656
                     /locus_tag="KJL99_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBD4437179.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYIGQCHTTQGNPYI
                     GVNFGVKTLDEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATSDPSYPQAIKTSRPGVGVVVTDSQHNIISPAGGTLPLSIPDDADSIRE"
     gene            3754..4294
                     /locus_tag="KJL99_002488"
                     /pseudo
     CDS             3754..4294
                     /locus_tag="KJL99_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            4302..4817
                     /locus_tag="KJL99_002489"
     CDS             4302..4817
                     /locus_tag="KJL99_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBD4437180.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            4783..5520
                     /locus_tag="KJL99_002490"
     CDS             4783..5520
                     /locus_tag="KJL99_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HBD4437181.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPALLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTNWCQ
                     VIAPCHVAWRTINDYGGLSAKKEQNLP"
     gene            5631..6641
                     /gene="pyrD"
                     /locus_tag="KJL99_002491"
     CDS             5631..6641
                     /gene="pyrD"
                     /locus_tag="KJL99_002491"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HBD4437182.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEDELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            6815..7357
                     /gene="zapC"
                     /locus_tag="KJL99_002492"
     CDS             6815..7357
                     /gene="zapC"
                     /locus_tag="KJL99_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HBD4437183.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKDQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(7354..8463)
                     /gene="ycbX"
                     /locus_tag="KJL99_002493"
     CDS             complement(7354..8463)
                     /gene="ycbX"
                     /locus_tag="KJL99_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HBD4437184.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            8707..10815
                     /gene="rlmKL"
                     /locus_tag="KJL99_002494"
     CDS             8707..10815
                     /gene="rlmKL"
                     /locus_tag="KJL99_002494"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HBD4437185.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERHKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            10827..12734
                     /locus_tag="KJL99_002495"
     CDS             10827..12734
                     /locus_tag="KJL99_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBD4437186.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLQEKEEALRVEELQNAEFDLKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVNGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRAFSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKSEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            12864..14117
                     /gene="pqiA"
                     /locus_tag="KJL99_002496"
     CDS             12864..14117
                     /gene="pqiA"
                     /locus_tag="KJL99_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HBD4437187.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            14122..15762
                     /gene="pqiB"
                     /locus_tag="KJL99_002497"
     CDS             14122..15762
                     /gene="pqiB"
                     /locus_tag="KJL99_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HBD4437188.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            15759..16322
                     /gene="pqiC"
                     /locus_tag="KJL99_002498"
     CDS             15759..16322
                     /gene="pqiC"
                     /locus_tag="KJL99_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HBD4437189.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            16578..16745
                     /gene="rmf"
                     /locus_tag="KJL99_002499"
     CDS             16578..16745
                     /gene="rmf"
                     /locus_tag="KJL99_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HBD4437190.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLSQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(16815..17333)
                     /gene="fabA"
                     /locus_tag="KJL99_002500"
     CDS             complement(16815..17333)
                     /gene="fabA"
                     /locus_tag="KJL99_002500"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HBD4437191.1"
                     /translation="MVDKRESYTKEDLLASGHGELFGAKGPQLPAPNMLMIDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDCRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(17402..19162)
                     /locus_tag="KJL99_002501"
     CDS             complement(17402..19162)
                     /locus_tag="KJL99_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HBD4437192.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRVQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            19347..19799
                     /gene="matP"
                     /locus_tag="KJL99_002502"
     CDS             19347..19799
                     /gene="matP"
                     /locus_tag="KJL99_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HBD4437193.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(19875..20921)
                     /gene="ompA"
                     /locus_tag="KJL99_002503"
     CDS             complement(19875..20921)
                     /gene="ompA"
                     /locus_tag="KJL99_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HBD4437194.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPEVQTKHFT
                     LKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS
                     ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEI
                     EVKGIKDVVTQPQA"
     gene            complement(21277..21786)
                     /gene="sulA"
                     /locus_tag="KJL99_002504"
     CDS             complement(21277..21786)
                     /gene="sulA"
                     /locus_tag="KJL99_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HBD4437195.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLTDDLTEEEHAELVDAANEGNAMGFIMRPVSASSHTTRQLSG
                     LKIHSNLYH"
     gene            22005..>22412
                     /gene="sxy"
                     /locus_tag="KJL99_002505"
     CDS             22005..>22412
                     /gene="sxy"
                     /locus_tag="KJL99_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HBD4437196.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEV"
BASE COUNT         5721 a         5257 c         5874 g         5560 t
ORIGIN      
        1 tatcgaacca tcaatgacta cggggcggaa acgccgcaac tcaactgtaa atcgtaagcc
       61 gtcttcagtt aagagagcga gatgtataga actcatcgac aacacagcct gttaagctct
      121 ggtggagtgc catcgtttat tggtgggctg gtggtgtttg tgtcggcagc gttcaatgca
      181 caagctgaaa cctggttcga tcctgctttt ttcaaagatg atccctcaat ggtggccgat
      241 ttgtctcgtt tcgaaaaagg acaaaaaata acgccagggg tttatcgtgt cgatattgtt
      301 ctgaatcaga caattgtaga tacgcgcaac gtcaattttg ttgagataac gccagagaag
      361 gggattgccg cctgtttgac gactgaaagc ctggatgcaa tgggtgttaa tactgatgcg
      421 tttccggctt ttaaacaact ggacaaacaa gtgtgtgtgc cattggcgga gattattccg
      481 gatgccagcg taacgtttaa tgtgaataaa ctccgtctgg aaatttcagt accgcaaatc
      541 gccatcaaaa gtaatgcccg tggttatgtc ccccctgaac gttgggatga agggatcaac
      601 gcgctattac tgggatattc gtttagcggg gctaacagta ttcatagcag cgcagacagt
      661 gattctggcg acagctattt tctgaattta aacagtggcg ttaatttagg cccatggaga
      721 ttacgcaaca attcaacatg gagtcgcagt agtggccaaa ccgcagaatg gaagaatctc
      781 agcagctatt tgcagcgggc ggttattcca ctaaaaggcg aactgaccgt aggtgatgat
      841 tatactgcag gcgatttttt cgatagtgtc agctttcgtg gtgtgcagct ggcgtcagat
      901 gacaacatgc tgccagacag cctgaaaggg tttgcgcctg tggtgcgtgg tatcgccaaa
      961 agcaatgccc agataacgat taagcaaaat ggttacacca tttaccaaac ttatgtatcg
     1021 cctggtgctt ttgaaattag tgatctctat tccacgtcgt cgagcggtga tttgttagtt
     1081 gaaatcaaag aagcggacgg cagcgtcaat agctacagcg taccgttttc cagcgtgcca
     1141 ttactccagc gtcaggggcg aatcaaatac gcggtgacac tggcgaaata cagaaccaat
     1201 agtaatgaac agcaggagag caaatttgcc cagaccacgt tgcagtgggg cggaccgtgg
     1261 ggaacgacat ggtatggtgg tggacaatat gctgaatatt accgtgccgc catgtttggt
     1321 ctgggattta accgtggcga tttcggagca atttcgttcg atgcgaccca ggcgaagagt
     1381 acgctggcag accaaagcga acataaaggt cagtcatatc gttttctgta tgccaaaacg
     1441 ctcaaccaat tgggcactaa ttttcaattg atgggctatc gctattcgac gtcgggtttc
     1501 tacacccttt ccgacaccat gtataaacat atggatggct acgaatttaa tgacggtgat
     1561 gatgaagata cgccgatgtg gtcgcgttat tacaatttgt tttacaccaa acgtggcaaa
     1621 ctgcaggtca atatctccca gcaattaggc gagtacggtt cgttttattt aagtggtagc
     1681 cagcaaactt actggcatac cgatcaacag gatcggctat tacagtttgg ctacaacacg
     1741 caaattaaag atctctcgct gggggtttcc tggaactaca gtaagtcccg tggtcaacct
     1801 gatgctgatc aggtgtttgc actaaatttt tccctgccgc tcaatctgtt gctccccaga
     1861 agtaatgata gctataccag gaaaaaaaat tacgcctgga tgacctctaa caccagtatc
     1921 gataacgaag ggcacattac acaaaacctg ggtttaacgg agacactact cgatgacggt
     1981 aacctgagct acagcgtgca acagggatat aacagcgagg ggaaaacggc taatggtagc
     2041 gccagcatgg actacaaagg ggcgtttgca gatgcccgag tgggctacaa ctacagcgat
     2101 aacggcagtc aacaacaact gaactacgct ctttcaggca gtttagttgc ccattcacag
     2161 ggcattaccc tggggcaatc gctgggggaa actaacgtgc tgattgcagc accaggcgca
     2221 gagaatactc gtgtggcgaa cagcaccggg ctgaaaactg actggcgcgg atataccgtt
     2281 gtcccctatg ccacttctta tcgggaaaat cgaatcgcac ttgatgcggc atcgttaaaa
     2341 cgtaacgtgg atcttgaaaa tgcagtagtc aacgtggttc ccaccaaagg ggcgttggtt
     2401 ctggcggagt tcaatgccca tgcgggagca agggtattaa tgaaaacatc aaagcagggt
     2461 ataccgctgc gttttggcgc gatagcgacg ctggacggca tacagactaa tagcggcatt
     2521 attgatgatg atggctcgct ctatatgtcc ggtttgcccg ctcagggagc gataactgta
     2581 cgctggggcg aagctcccga tcaaatttgt catatcagtt accagcttac cgaacaacaa
     2641 attaactctg cgattacgcg aatggatgcc atatgcagat aatctttgga gaaaaatgcg
     2701 tgttattact acgactattt tttgccgccg tcttaatgct atggtgcgct caaactgctg
     2761 cttatatcgg gcagtgtcat accactcagg ggaatccgta tattggcgtc aattttggcg
     2821 ttaaaactct ggatgaagaa gaaaatacgg ctggagtagt taaagacaaa ttttatcagt
     2881 ggaacgaatc gaatgattat tatgtttcct gtgattgcga taaagacaat gtcagaagtg
     2941 gccgatgggc attcgccgcg gattcaccgt tagtctattt aggcgacaac tggtacaaaa
     3001 ttaatgacta tcttgccgcc aaagttttat tgcaggttaa aggcagttct cctactgcgg
     3061 ttcctttcga aaacgtgggc acaggggcag atacacgatg gcatatttgc gatcccggcg
     3121 gtcaacgttt aggtggccag ggggctagcg gtaatagcgg tagcttttcc ctgaaaatat
     3181 tgcagccgtt cgttggttcg gtcgtcattc ctcctatggc gctggcgcga ttatttgaat
     3241 gctacaacat acccgcaggt gattcctgca cgactactgg tacaccggtt ttagtgtatt
     3301 acctgtctgg tacgatcaat tcacttggct catgttccgt caatgccgga gaaacaatcg
     3361 aggttgatct gggcgacgta tttgcggcta actttcgtgt tgtagggcat aagcctcttg
     3421 gggccagaac ggcagaactt gcaattccgg tcaggtgtaa cacgggaaac gcagggttag
     3481 ttaacgtcaa tctgagtctg acggcaacct cagaccccag ctatccccag gcgattaaga
     3541 cgtcacgtcc tggcgtgggc gtggtggtga ccgatagcca gcacaacatt atttcccctg
     3601 ctggtgggac actaccgctc tctattcctg atgatgcaga cagtatccgc gaatgaatgt
     3661 ctatccagtc agcacgacaa gcgtaccacc agaaaccggg cgatttgaag ccacggcaac
     3721 ggtgagaata aattttgatt aacgcgtgaa cgtatgaaga aaaaaacgat atatcagtgc
     3781 gttattttgt tctttagcct tcttaacatc catgtcggga tggctgggcc tgaacaagtc
     3841 agtatgcata tttatgggaa tgtggtcgat cagggctgtg atgtcgccac caaaagtgca
     3901 ttacaaaata ttcatattgg tgattttaat atcagtgatt ttcaggccgc gaataccgta
     3961 agcactgctg ctgatttgaa tattgatatc accggttgtg ccgctggtat tactggcgcg
     4021 gacgtccttt ttagcggcga ggctgacacc cttgcgccga cactgctcaa actaactgac
     4081 acaggcggaa gcggtggtat ggcaacgggg attgccgtgc attcttgatg cgcaaagtca
     4141 gcaagaaatc ccgctcaatc aggtccagcc tcttacgccc ttaaaagccg gggataacac
     4201 actcaaatat caacttcgtt ataagtccac aaaggcggga gcaacgggcg gtaatgcgac
     4261 ggcggttctc tattttgatc tggtttacca gtgaaggagt gatgttgaaa cggataatct
     4321 ggattctgtt cttactggga ttaacggggg gctgtgagct atttgcccat gatggcacgg
     4381 tcaacattag cggatcgttt cgccgtaata catgcgtgct ggcacaggat agcaagcaaa
     4441 tcaatgtgca gttaggcgat gtctcgctga cacgtttttt tcatgggaat tatggcccgg
     4501 agaaatcttt catcattaat ttgcaggatt gcggaacaga tgtcagcacg gttgatgtca
     4561 ccttttcagg aacccccgac ggagtgcaga gcgaaatgct gtccattgaa agtggtacgg
     4621 atgcagccag tgggctggca attgcgattc tggatgatgc gaagatattg attccgctta
     4681 accaggcgag taaagactac agcctacata gcggtaaggt gccattaact ttttatgcgc
     4741 aattgcgacc tgtcaatagc gatgtgcagt cgggtaaagt gaatgccagc gcgacatttg
     4801 tacttcatta tgactaatac gtggaacaga ttagcgctct tgattttcgc cgttttatcg
     4861 ctgctggtgg cgggtgaatt gcaggcagga gtggtggtcg gcggaacgcg atttatcttt
     4921 ccggcagaca gagaatcgat atctatttta ctgactaata ccagtcagga atcttggctt
     4981 attaacagca aaatcaatcg cccaacgcgt tgggccgggg gggaagcgtc gacagtgcca
     5041 gcactattac tggccgctcc gccacttatt ctcctgaagc ccggtactac aggcacgttg
     5101 cgcttgctga gaacggaaag cgacatcttg cctgtggatc gcgaaacgct atttgagtta
     5161 agcattgcca gcgtgccatc cggcaaagtt gaaaatcaga gcgtaaaagt ggcgatgcgc
     5221 tcggtattta aactgttctg gcgacccgaa ggtttgccgg gcgacccgct ggaagcttat
     5281 caacaattac gctggacacg gaattcgcag ggtgtacaac tcactaaccc aacgccttac
     5341 tacattaacc tgattcaggt gagcgtaaat ggtaaagcgt taagtaatgc gggagtcgtg
     5401 ccgcctaaaa gccagcgtca gacaaactgg tgtcaggtaa tagcaccgtg tcatgtcgcc
     5461 tggcggacga taaatgatta cggcgggttg agtgcaaaga aggagcaaaa tctgccctga
     5521 aacaggttct gaaaacgttt gcgttttttt tgccgcaggt caattccctt ttggtccgaa
     5581 ctcgcacata atacgccccc ggtttgcaca ccgggaatcc aggagagttc atgtactacc
     5641 ccttcgttcg taaagccctt ttccagctcg atccagagcg cgctcatgag tttacttttc
     5701 agcaattacg ccgcattacc ggaacaccgt ttgaagcact ggtgcggcag aaagtgcctg
     5761 cgaaacctgt taactgcatg ggcctgacgt ttaaaaatcc gcttggtctg gcagccggtc
     5821 ttgataaaga cggggagtgc attgatgcgt taggcgcgat gggatttgga tcgatcgaga
     5881 tcggtaccgt cacgccacgt ccacagcctg gtaatgacaa gccgcgtctc tttcgtctgg
     5941 tagatgccga aggtttgatc aaccgtatgg gctttaataa tcttggcgtt gataacctcg
     6001 tagagaacgt aaaaaaggcc cattatgacg gcgtcttggg tattaatatc ggcaaaaata
     6061 aagatacgcc agtggagcag ggcaaagatg actatctgat ttgtatggaa aaaatctatg
     6121 cctatgcggg atatatcgcc atcaatattt catcgccgaa taccccagga ttacgcacgc
     6181 tgcaatatgg tgaagcgctg gatgatctct taaccgcgat taaaaataag caaaatgatt
     6241 tgcaagcgat gcaccataaa tatgtgccga tcgcagtgaa gatcgcgccg gatctttctg
     6301 aagacgaatt gatccaggtt gccgatagtt tagttcgcca taatattgat ggcgttattg
     6361 caaccaatac cactctcgat cgttctctgg ttcagggaat gaaaaattgc gatcaaaccg
     6421 gtggcttaag tggtcgtccg cttcagttaa aaagcacaga aattattcgc cgcttgtcac
     6481 tagaattaaa cggtcgctta ccgatcatcg gtgttggcgg catcgactcg gttatcgctg
     6541 cgcgtgaaaa gattgctgcg ggtgcctcac tggtgcaaat ttattctggt tttattttta
     6601 aaggtccgcc gctgattaaa gaaatcgtta cccatatcta attatttctt cgaattcctc
     6661 tttataagat aatcagggct ttattttcag acctggttgt tttatattca tcgttgttgc
     6721 ttatttagac attttgtact tttattgttg aggttattaa gcgaagcgac aatggattgt
     6781 gttgctgcgg ttttataggg taggggagag gcagatgcga attaaaccag acgataactg
     6841 gcgttggtat tacgatgaag agcacgatcg aatgatgctc gatttagcca atggtatgct
     6901 atttcgctca cgttttgcgc gcaaaatgtt gactccagat gccttttctc ccgcaggctt
     6961 ttgtgttgat gatgccgcgc tctatttctc ttttgaagaa aagtgccgcg attttaattt
     7021 atctaaagac caaaaagccg aactggtgct gaatgcactg gtagcaattc gttatctcaa
     7081 accgcaaatg ccgaaaagct ggcattttgt ctcccatggt gaaatgtggg tgcccatgcc
     7141 gggcgatgcg gcctgcgttt ggttgagcga tactcacgag caggtcaatt tgctggtcgt
     7201 tgaatctggc gaaaatgccg cgctatgcct gctggcacaa ccctgcgttg ttattgcggg
     7261 tcgcgcgatg cagttgggcg acgccattaa aatcatgaac gacaggctga aaccgcaggt
     7321 gaatgttgac agcttcagcc tcgaacaggc agtctaacgc gtcagcttaa gggcagtctt
     7381 cggtacacag ctacagcaaa gaatggtgcc atcatcgccc attgctgatt ttttcagcgg
     7441 cgtgacttcg ccttctaaaa gctgaacacg gcaacttcca caaatgcccg cgcggcaaga
     7501 ataagggata cgaattccct gattttctaa ttgctccagc aacacttgtt ggttatttcc
     7561 acgaaatgcc tgtccctgcc agtcaatatc tacatttgcg tccggttgtt gcgtgatgtt
     7621 ggcggtatca tcagcggcag ctgcgccgta aatttttgcc ggagccgttg ccagaatttc
     7681 cacctcatcg ccaacgcgaa tcacgccgct attacgggca attaaattct gaccaaaatc
     7741 gacatcgccg ttatcctggg cagtgcggaa agattgcaat gtttttaatg gttcgcctgc
     7801 cggatgtttt tgcccttttt ctgggctgac ggtggtgaaa atacagcggc tacaaggttt
     7861 aaccacatca aacaccacat caccaatgcg aatcactttc cagctatctt cttcccaggc
     7921 tgacgcgccg gaaaccacca gattggggcg gaattgctcc atttttacac tggccggaca
     7981 acgttgttgg agatcacgta acgaggcttc gttagcaaga aggtaaggat agccatcagc
     8041 aaatgacaga ggtacagtgt tgtggcgttt cacgcgccgg gtcatttgtg gccccaccca
     8101 gcgtaattgc acttcgcggg agaaaaatcc acttagccat ttgttgatcg cgtctggcgc
     8161 aattcgcgcg gtaaaatgtg tgccccaaac ttcggttggt gcgtcttgtg tggcgaaatc
     8221 agcaaaacga acatatgcgc tactgccatc tggtgcggtg agatgcaagc catcatgtac
     8281 aggtgaagga gtaaaccgta ccatctgggg aaactggcga gcggtaataa acgtaccgtc
     8341 aggttccgtg atcataaaga tgcgatcgaa ggccagacca ctgacatcag ccagagcatg
     8401 tgtaagacca atgccgcgca tcgatttaac aggatgaata aaaagccggg ttaatgtcgc
     8461 cacggtgcgg tcctcaaatg aaaataagcc ctcaacttta tgacatgacg cgcttattag
     8521 ctataatgcg caacaatttt cttagcgcct gtcccgttag gctattttat ttgtcagttt
     8581 ggccctgggt tgtgctcgaa atcctcacgt acttgtgtac gctccggttt ctccgcgctc
     8641 tccatgtcca gactgactgc aacaattacg cctactgcgg taggctctta gagtaaaagt
     8701 gacgatatga attctctgtt tgccagtacg gcccgtgggc tggaagagct gttaaaaact
     8761 gaactggaaa atctgggggc cgttgaatgc caggtggttc agggtggggt ccatttcaag
     8821 ggcgacacac ggcttgttta ccagagcctg atgtggagcc gcctggcctc gcgtattatg
     8881 ttgccgctgg gcgagtgtaa ggtttacagc gatttagacc tctatctcgg tgttcaggcg
     8941 atcaactgga cagagatgtt taatcctggc gcgaccttcg ctgtccactt cagtggtttg
     9001 aatgacacca tccgcaacag tcagtacggc gcgatgaaag tgaaagacgc gatcgtcgat
     9061 gctttcacac ggaaaaatct gccgcgtcca aatgttgatc gcgatgcgcc ggatatccgc
     9121 gttaacgtct ggctgcataa agaaaccgcc agtatcgctc ttgatctgag tggtgatggt
     9181 ttacatctgc gtggctatcg cgatcgtgct ggtattgcgc cgatcaaaga aaccctggca
     9241 gccgctattg tgatgcgatc cggctggcag tcaggaacac cgctgctcga tccgatgtgt
     9301 ggttccggta cgttgctgat tgaagcggcg atgctggcga ccgatcgcgc accaggcttg
     9361 caccgtgggc gttggggctt tagcggctgg gcgcagcatg atgaagctat ctggcaggaa
     9421 gtgaaagcgg aagcgcaaac tcgcgcccgt aaaggcctag ctgagtatag ctctcatttt
     9481 tacggttcgg acagcgacgc acgggtgatt caacgtgcgc gcactaacgc ccgtcttgcg
     9541 gggattggtg aactgatcac ctttgaggtg aaagatgtcg cgcaactgac caatccgctg
     9601 ccgaaagggc cgtacggtac agtgttgagc aacccgccat acggtgaacg tctggacagc
     9661 gaaccggcgc tgattgcgct gcatagtctg ctgggccgaa tcatgaaaaa ccagttcggt
     9721 ggctggaatc tctctttgtt tagtgcctcg ccggatctgc taagctgcct gcagctgcgt
     9781 gcagacaaac agtacaaggc gaaaaacggc ccgctggact gcgtacagaa aaattaccac
     9841 gttgccgaaa gcacaccaga cagtaaaccg gcaatggtag cggaagatta cgccaaccgc
     9901 ctgcgtaaga acctcaaaaa attcgagaag tgggcacgcc aggaagggat tgaatgttac
     9961 cgcctgtatg acgccgatct gccggaatat aacgttgccg ttgaccgtta tgccgactgg
    10021 gtggtggtgc aggagtatgc gccgccaaaa actattgatg cgcacaaagc gcgtcagcgt
    10081 ctgttcgata ttatcgctgc aaccatttcg gtactgggga ttgcgccaaa caaactggtg
    10141 ctgaaaaccc gtgaacgcca taagggcaaa aaccagtacc agaaactggg cgagaagggc
    10201 gagtttcttg aagttaccga atataacgct cacttgtggg tgaacctgac ggattatctc
    10261 gatactggtc tgttcctcga tcaccgtatc gcccgccgta tgctcggtca gatgagcaaa
    10321 ggcaaagatt tcctcaacct gttttcttat accggcagcg ctaccgtgca tgcgggatta
    10381 ggcggtgcac gcagcaccac caccgtggat atgtcgcgta cttatctgga atgggcagaa
    10441 cgcaacctgc gtctgaatgg cctgactagg cgtgcgcatc gcctgattca ggccgattgc
    10501 ctggcgtggc tgcgtgaggc aaatgaacag ttcgatctga tctttatcga tccgccaacc
    10561 ttctctaact caaaacgaat ggaagatgcg tttgatgttc agcgcgatca tctggcgctg
    10621 atgaaagatt tgaaacgtct gctgcgtgca ggtgggacga tcatgttctc gaacaacaaa
    10681 cgtggcttcc gtatggatct cgacggcctg gcaaaactgg gactgaaagc acaagaaatt
    10741 acgcaaaaaa cgctctccca ggatttcgcc cgtaaccgcc agatccacaa ctgctggctg
    10801 attaccgcag cctgaaagga atagtaatgt cattaatcag tatgcatggc gcatggctgt
    10861 cgttcagcga cgcgccgctt ctcgataacg cagaactgca tatcgaagat aacgaacgtg
    10921 tttgtctggt gggccgcaac ggcgcaggaa aatcgacgtt aatgaaaatc ctcaaccgtg
    10981 aacaagggct ggatgacggt cgcattattt atgagcaaga tttgattgta gctcgtctgc
    11041 aacaggatcc gccgcgtaac gttgagggta gcgtttatga tttcgttgcc gaaggcattg
    11101 aagaacaagc ggaatatctg aaacgctatc acgatatttc gcgcctggtg atgaacgacc
    11161 cgagcgagaa aaatctcaac gaactggcga aggttcagga acagctggat caccacaacc
    11221 tgtggcagct ggaaaaccgc atcaacgaag tgctggcgca actggggtta gatcctaacg
    11281 ttgcgctgtc gtcgctttcc ggcggctggt tgcgtaaagc ggcattagga cgcgcgctgg
    11341 tgagcaatcc gcgcgtattg ctgctggacg aaccgactaa ccatctggat atcgaaacca
    11401 tcgactggct ggaggggttc cttaaaacct tcaatgggac gattattttc atctcccacg
    11461 accgttcgtt tatccgcaat atggcgacgc gcattgttga tctcgatcgc ggcaagctgg
    11521 tgacctatcc agggaattac gaccagtacc tgcaggaaaa agaagaagcc ctgcgcgtgg
    11581 aagaactaca aaatgccgag ttcgatctca aactggcgca ggaagaagtg tggattcgcc
    11641 aggggatcaa agcacgccgt acccgtaatg aaggccgcgt acgcgccctg aaagcgatgc
    11701 gtcgcgaacg tggtgaacgt cgcgaagtga tgggtaccgc aaagatgcag gtggaagagg
    11761 ccagccgctc cggtaagatc gttttcgaaa tggaagacgt ttgctaccag gttaacggta
    11821 agcaactggt gaaagatttt tctgcccagg ttctacgtgg cgacaaaatt gccctgattg
    11881 gtccgaatgg gtgcggcaaa accacgctgc tgaaactgat gctcggtcag cttcaagcgg
    11941 acagcgggcg tattcacgtt ggcaccaaac tggaagtggc ttatttcgat cagcaccgcg
    12001 cggaactgga tcccgataaa acggtgatgg ataaccttgc cgaaggtaag caagaggtga
    12061 tggttaatgg caagccacgc cacgtattgg gctatttgca ggactttctg ttccatccga
    12121 aacgggcgat gacgccagta cgtgcgtttt ctggcggtga gcggaaccgc ttgctgctgg
    12181 cgcgtttgtt cctcaaacca agcaacttat tgattcttga cgaaccgacc aacgatcttg
    12241 atgtcgaaac gctggaactg ctggaagaac tgatcgacag ctatcagggc acggtattgc
    12301 tggttagcca cgatcgtcag tttgtcgata acaccgttac agaatgctgg atcttcgaag
    12361 gcggcggtaa aattggtcgt tatgttggcg gttatcatga tgcccgtggt cagcaagaac
    12421 aatatgtggc actcaaacag cctgcggtga aaaaaagcga agaagccgcc gcgccaaaag
    12481 cagaaactgt aaaacgcagc agtagcaaac taagctataa attgcagcgc gaactggagc
    12541 agctaccgca attgctcgaa gatctggagg cgaagctgga agccctacag acgcaagtgg
    12601 cggatgcttc cttcttcagt cagccgcatg agcagacgca aaaagtgctt gctgatatgg
    12661 ctgctgcaga gcaggagctg gagcaagcct ttgaacgctg ggagtatctt gaagcgttaa
    12721 aaaatggtgg ctgatcgcaa aatagtcgat ttaaacagag acagctgaca acggtaaaat
    12781 tgcctgatga actgggttta tcaggcatga aaaccaacac tttgttagtc atctgtaaaa
    12841 tagcgcatca ttaaggagta ccaatgtgcg aacatcatca tgccgcgaag cacatcctgt
    12901 gctcgcagtg tgacatgctg gtggcgttac cgcgccttga gcatggtcag aaagcggcat
    12961 gtccccggtg tggcacaacg ttaaccgtgg cgtgggatgc ccctcggcag cgtcccaccg
    13021 cctatgcgtt ggctgcactg ttcatgctgt tgctgtccaa cttgtttcct tttgtgaata
    13081 tgaacgttgc aggagttacc agtgaaatta cattactgga aattcccggc gtgctttttt
    13141 ctgaggacta cgccagcctc ggcacctttt tcctgttgtt tgtgcaactg gttcccgcgt
    13201 tttgtctgat aaccattctg ttactggtga atcgcgcgga attaccggtc cgtttaaaag
    13261 agcaactggc acgggtgctt tttcaactca aaacctgggg aatggcggag attttcctcg
    13321 cgggtgtgct ggtcagtttc gttaaactga tggcttacgg cagcattggg gtaggcagca
    13381 gctttctccc ctggtgttta ttttgtgtcc tgcaactgcg cgcttttcag tgcgttgatc
    13441 gtcgctggtt atgggacgac atcgccccga tgccagaact gcgccagccg ctaaaaccag
    13501 gcgtcacggg gatacgtcag gggctgcgtt cgtgctcctg ttgtacggca atccttcctg
    13561 ctgatgaacc cgtgtgcccg cgttgtagta ccaaagggta cgttcggcgt agaaacagcc
    13621 tgcagtggac actcgcgctg cttgtaacgt ccatcatgct gtatcttccg gctaatattt
    13681 tgcccatcat ggtgacggat ttattaggct cgaagatgcc gtcgacgatt ctcgctgggg
    13741 tcattctgtt atggagcgaa ggctcttatc ccgtcgctgc ggtgatcttt ctggccagta
    13801 ttatggtgcc aacgttaaag atgatcgcca tcgcgtggct gtgttgggat gccaaagggc
    13861 atggcaagcg cgacagtgaa agaatgcatt tgatttatga agttgttgag tttgtaggcc
    13921 gctggtcgat gattgacgtt ttcgttatcg cggtgctctc ggcgctggtg cgtatgggag
    13981 gtttaatgag tatttatccg gcaatgggtg cattaatgtt tgctttagtc gtcataatga
    14041 caatgttttc tgctatgacg tttgacccgc gtttgtcgtg ggatcgtcaa cctgaatcag
    14101 agcatgagga gtcctgacag tatggaatct aataatgggg aagccaaaat ccagaaagtg
    14161 aagaactggt ctcccgtgtg gatatttcct atcgtcacgg cgctcattgg ggcctgggtt
    14221 cttttttatc attacagcca tcagggaccg gaagtgaccc tgatcaccgc gaatgcggaa
    14281 ggaattgaag gtggcaaaac caccattaaa agccgtagcg ttgacgtcgg cgtggttgaa
    14341 agcgccacac tggctgatga tttgacgcac gttgaaatca aagcgcggct gaattccggt
    14401 atggaaaaat tgctgcataa agacaccgtc ttttgggtgg tgaaaccgca gattggtcgc
    14461 gaagggatta gcggcctggg aacgctgctg tctggagttt atatcgaact gcagccaggc
    14521 gcgaaaggca gcaaaatgga taaatacgat ttgctggact caccaccgtt ggccccgcct
    14581 gatgcgaaag gtatccgtgt gattctcgat agcaaaaaag ccgggcagct ctcgccagga
    14641 gatccggtgc tgttccgtgg ctatcgggta ggttcggttg aaaccagcac cttcgatacg
    14701 caaaaacgca atatcagtta tcaactgttc atcaatgcac cttatgaccg actggtgacc
    14761 agcaatgttc gcttctggaa agatagtggc attgcggttg atctgacgtc agcggggatg
    14821 cgtgtggaga tgggctcatt gacaacgctg cttagtggcg gtgttagctt tgatgtgccg
    14881 gaaggtctgg atttagggca gccagtggca ccgaaaacag ctttcgtttt gtatgatgat
    14941 cagaagagca ttcaggattc gttgtacacc gatcacattg attatctgat gttctttaaa
    15001 gattcggtac gcggtctgca accgggagct ccggtagaat tccggggtat tcgcctgggt
    15061 accgtaagca aagtgccatt ctttgcgccg aatatgcgtc agacatttaa cgatgattac
    15121 cgtattccgg tactgattcg tatcgagcca gagcggctga aaatgcagct tggcgaaaat
    15181 gcggatgttg ttgagcacct tggcgaattg ttgaaacgtg gtttacgcgg atcgctgaaa
    15241 accggaaacc tggtcactgg tgcactgtat gttgatctcg atttctatcc aaatacgcct
    15301 gcaataaccg gtattcgtga atttaatggt tatcagatta tcccgactgt tagcggcggc
    15361 ttggcgcaaa tccagcaacg actgatggaa gcgttggata agatcaacaa actgccattg
    15421 aatccgatga ttgaacaggc aaccagtacg ctttctgaaa gtcagcgcac aatgaaaaac
    15481 ctgcaaacga cgctggatag catgaacaag atcctcgcca gccagtcgat gcagcagttg
    15541 ccgacggata tgcagtcaac gttgcgtgaa ttgaatcgca gcatgcaggg cttccagcct
    15601 ggctccgcag cctacaacaa gatggtggcg gatatgcagc gccttgatca ggtgttgcga
    15661 gaactgcaac cggtgctgaa aacgctcaat gagaagagta acgcgctggt atttgaagcg
    15721 aaggacaaaa aagatccaga gccgaagagg gcgaaacaat gaaaaagtgg ctagtgacga
    15781 ttgcagcact gtggctggcc ggatgcagct ccggcgaaat taataaaaac tattaccagt
    15841 tacctgtggt gcagagcggt acacaaagta ccgccagcca gggcaatcgt ctgttatggg
    15901 tagagcaggt cactgttcct gactatctgg cggggaatgg tgtggtttat caaaccagtg
    15961 atgtgaagta tgtgattgcc aacaacaact tgtgggccag cccgttggat caacagttgc
    16021 gcaacaccct ggttgccaac ctgagtacgc aactgcccgg ctgggtggtt gcctcccagc
    16081 ctctgggaag cgcccaggac acgctcaatg ttaccgtaac ggagtttaac ggtcgctatg
    16141 atggcaaggt cattgtcagt ggtgagtggc tgttgaacca ccagggacaa ctgatcaaac
    16201 gtccgttccg tctggaagga gtgcaaactc aggatggtta cgatgagatg gttaaagtgc
    16261 tggccggtgt ctggagtcag gaagccgctt ctattgcaca agagataaag cgtctacctt
    16321 aattataaag atttgtaaat ataaccgtct ccggtatgtt gcctgaggcg gtttttttgt
    16381 ttctaacgtg cggaaaaatt tgttcctctt cacatttttt gtacaaccga catgcccgtg
    16441 tagctcacaa atatgacagt ggcgtgaatt ttgcgcattg acggcagtta tgattcgcgg
    16501 tattgcttaa ctgtgattgc acatttagta atcactgttt tcttttccac cagaaaccag
    16561 tatgagggaa acgaggcatg aagagacaaa aacgagatcg cctggaacgg gcacatcaac
    16621 gtggttatca ggccggcatc gccggacgct caaaagaaat gtgtccctat cagacgctga
    16681 gtcaaaggtc acaatggctg ggaggctggc gagaagccat ggcggacagg gtagtaatgg
    16741 cctgattctg tctctttaaa aagaaacctc cgcattgcgg aggtttcgcc ttttgatact
    16801 ctgtctgatg ataatcagaa ggcagacgta tcctggaaca gaccgacttt caggtcgctg
    16861 gcggtataga tcagacgaca atcaaccagc acttcgccat ctgccagacc cataatcaga
    16921 cgacgattaa caatgcgttt aaagtgaata cggtaggtca cttttttcgc tgtcggcagt
    16981 acctgaccag tgaatttcac ttcgccaacg cccagcgcgc ggcctttacc ttcgccgccc
    17041 agccagccga ggtagaaccc taccagctgc cacattgcgt ccaggcccag gcatcccggc
    17101 ataaccggat cgccaatgaa gtggcatccg aagaaccaca gatccggatt gatatccagt
    17161 tctgcttcaa catacccttt gtcgaagtta ccacccgttt cggtcatttt gaccacacgg
    17221 tctatcatca gcatgttcgg tgctggcaat tgcgggcctt tagcgccaaa cagttcaccg
    17281 tgaccagagg caagaaggtc ttcttttgta taggattcgc gtttatctac catgttctct
    17341 gtaagcctta ttttattgaa gcacgcagga tagctaacac gtgtacgctg aacaagtccg
    17401 atcagttcgg aataaaccag ttcagccaac gtaatggcca tggaaaacgg tgacgtcctt
    17461 cctgttgcga tgcttgcgcg atacgttcct ggatggtttg catcagcgtc gtttggcctt
    17521 cgccatccca caccagattt aataataacg gcagtgcgtc agtcacatcg tctactgccc
    17581 agatggtgaa tttgccttct tctaccgctt tcaccagttc actgtgaaga cttaaatggc
    17641 gaacgttagc agtggggata atgacacctt gtttccccgt taactcacgt tgctggcaaa
    17701 tagcaaagaa gccttcgatt ttctcattta aaccaccgac cggctggacg cggccgaact
    17761 gatcgactga acctgtgata gcgatactct gattcactgg cacatcagcg agggcgctta
    17821 tcagggcgca gagttcagcc atcgaggcac tatcgccatc cacttcactg tatgactgct
    17881 caaatgtcag cgatgctgag aaggggatct gttgctcaag ctgtagttcc gacatcagga
    17941 acgcctgcat gatcatcatc cctttcgcat ggatattgcc gccaagctcc gctttgcgtt
    18001 cgatgtcggt gaattcacca tcgccaatat gcacaacgca gctaatgcga gaaggttcgc
    18061 caaaagcgcg tggatgaccc ggaaattcaa tgaccgaaag ggcgttaatt tgcccgatgc
    18121 gttcgccttc ggtttcaatc aggatttgct cctgaaggat ctcatcctgc atacgttcag
    18181 cgaggaaacc ttcgcgccat tcacgctgct gcagcattaa gtttagctgc tcgccggaga
    18241 aggtgtcgcc atcacacagg gaggcgacct ctttacactg gcggaggatc cactgcgggc
    18301 taagcggaag tgtttcttgt tcaccggtgt agcgtgctgc ttcgcggata agtaccggcc
    18361 aagcatccgc tcccggggca ggtaagtgat tatgtctggc ggtaaatgtc acccagcgac
    18421 accactggct tactgactcc gcatcgacaa tctgcagagt atcttcaaat tcgctataaa
    18481 tagcctgctc tgaaagctct ggctccatct cctggaaatc agccaatgat tcgcgttcgc
    18541 ctaccagaat gactttcagc ttcaatggca ttgaaggcac agagacgggg agagggcgcg
    18601 actcatcaaa cgcaacccag tcaaaacgct cgcggttaac gatatttttc agccgcatcc
    18661 acagcagagg ttgcgccagc agtgtacgca aagagatgat gagaataccg ccatttgcct
    18721 gatgcaccag accaggctgc agggtaatgt cgccattaaa ctggcgcagg cagccaaaga
    18781 gttgctccgc ttctacccag tcggcagcga caacttgcgt taaagtcgca aaattatcat
    18841 ctgcactcac tgcgtgacgt aagcggatgg agtggccgga aacctcatag tgatcgccca
    18901 ccagttggcc tgcatcgctt tgtagcgtac gcgcggcatc ggcaataaga ttcagatact
    18961 cagactcttc cggggcctta gccagcataa aagaggagga tgctcgcgta tgcagcaact
    19021 gctccagcgc aaattgcagt cgcggttgag tatcactgaa taaaggatcg ttttcgtcaa
    19081 tcaaatgtgg ctgagcaaat atttcctgat agctatcggt atcaggaacc aggtcacgcc
    19141 atgcaagttt cgtaatggtc aaagttgatg ttttttagtc tgttgtcaaa gccgcgatta
    19201 taccgtaacc ggcactacag cacacgtaga aagcaccgac aatactcctg gctgggcgtt
    19261 aaagctcaca ggatggagat tcttttcttc actggcctaa aaagctgata ttctgtaaag
    19321 agttacacgg taacattgag atcgctatga aatatcaaca acttgaaaat cttgaaagcg
    19381 gttggaaatg gaagtatctg gtgaagaagc atcgcgaagg ggagttaatt acccgttaca
    19441 tagaagccag tgccgcccag gaagccgtcg atgtgttgct ctcgctcgaa aatgaacccg
    19501 tgctggtaaa tggctggatt gacaagcata tgaatccgga actggtcaat cgcatgaagc
    19561 agaccattcg ggccagacgg aagcgccatt ttaatgcgga acaccagcat acgcgcaaaa
    19621 aatcgatcga tctggaattt attgtctggc aacgtctggc tggtcttgca cagcgtcgcg
    19681 gtaaaacgct ttctgaaacg attgttcagc tgattgaaga tgcggaaaac aaagagaaat
    19741 acgcgaataa aatgtcttct cttaagcagg atctgcaggc attgctgggt aaggaataac
    19801 tgacgaaagt cagttcaatt tactaaaggc aaaaaaaacc ccgcagcagc ggggtttttc
    19861 taccagacga taacttaagc ctgcggctga gttacaacgt ctttgatacc tttaacttcg
    19921 atctctacgc gacgatccgg agccaagcag tcgatcagtg cagcacgctg tttcacgttg
    19981 tcacaggtgt tgccagtaac cgggttggat tcgcccatac cacgtgcgga gatcttgtct
    20041 gccgggatac ctttggagat caggtaatca acaacagact gagcacggcg ctcggacaga
    20101 ccctggttgt aagcgtcaga accgatgcgg tcagtgtaac ccagaacaac tacggaaccg
    20161 tctttcggat ctaggttgct cagctggctg tacagctgat ccagagcagc ctgaccttcc
    20221 ggtttcaggg ttgctttgtt gaagttgaac agaacgtcag acttcagagt gaagtgcttg
    20281 gtctgtactt ccggtgccgg agctggagca actaccggag ctgcttcgcc ctgaccgaaa
    20341 cggtaggaaa cacccaggct cagcagaccg ttgtccggac gagtaccgat ggtgtttgcg
    20401 tcaccgatgt tgttggtcca ctggtattcc agacgggtag cgatttcagg agtgatcgcg
    20461 tactcaacac cacctgcgaa aaccggagaa acgccagtgt cgtggtcttt aaaggatgcg
    20521 ccaccaggta cgttagcctt ggtgtctgca cgccatacca taccacccag acgagtgtag
    20581 atgtccagat cgtcagtgat tgggtaaccc agtttagcgg tcagctgaac gccctgagct
    20641 ttgtatgcgc cgttgatgtt gtcgcctttg tacggcatac gacctaacca gtcgtaaccc
    20701 atttcaaaac caacatacgg gttaacctgg taaccaccaa aagcacctgc acccagttgg
    20761 ttttcgtggg tcggaccatt gttaggaata aaaccagtgt catggtactg ggaccagccc
    20821 agtttagcac cagtgtacca ggtgttatct ttcggagcgg cctgcgctac ggtagcgaaa
    20881 ccagccagtg ccactgcaat cgcgatagct gtctttttca ttttttgcgc ctcgttatca
    20941 tccaaaatac gccatgaata tctccaacga gataacacgg ttaaatcctt caccggggga
    21001 tctgctcaat attaactcta ccgatatctt cggcttatgc cgagcacccc tggcgatgta
    21061 aagtctacaa cgtagttgaa aacttacaag tgtgaactcc gtcaggcata tgaaaaaaaa
    21121 gtcttgtata aggtatgttt aatctttttg tcagcgacaa tttacagaaa aaaaacgcgg
    21181 aaaccgcttc agacaagcct tcgcaaggaa aattagtcac gactgaaagc attggctggg
    21241 cgacaaaaaa agttctagga ttaatcctaa atttacttaa tgatacaaat tagagtgaat
    21301 ttttagcccg gaaagttgtc tcgttgtgtg agaggatgcg cttaccggac gcataataaa
    21361 ccccatagcg ttaccttcat ttgccgcatc aacaagttca gcatgctctt cttcagtcaa
    21421 atcatctgtc aaccaaccga tcaccacact gtaattgccc gtgcgtaaag cgcgaaccat
    21481 tgactccact gtgtggcaag gggagagctg gctaatctgc attactttcg ttaagggtag
    21541 cccagatgcc tgaacccatt cccgactcag tttttgttgc ggtgttaacc agagttgcca
    21601 gcgcgattgc tgaccgagtt gctgtaacaa tggcaacagt agaagttgcg tcatcatggg
    21661 ctgatcttcg cgatagacaa cttcactgat aagcccggct gtagtgtttt ccgtagagac
    21721 acgcgcaatt ttacttgctg cggatgagaa cgacgaagaa cgatgtgcat agcctgaagt
    21781 gtacataatc aatccagccc ctgtgagtta ctgtatggat gtgcagtaca tccagtgaca
    21841 acaaagatca accctatttt cggaaagagc ctcgcaaatt ttgtcgttgg tgacgggaaa
    21901 acataaatta atcttgcccc ttaagaataa gttgcctatt ttcgtagtta acggatccgt
    21961 taatgtgaat cattctttta tgttatgatt ttaaaaggaa ttttatgaaa agcctctcct
    22021 ataagcggat ctataaatca caagaatacc tggcaacgtt gggcacaatt gaataccgat
    22081 cattgtttgg cagttacagc ctgaccgttg acgacacggt gtttgcgatg gtttctgatg
    22141 gtgagttgta tcttcgggct tgtgagcaaa gtgcacagta ctgtgtaaaa catccgcctg
    22201 tctggctgac atataaaaag tgtggccgat ccgttaccct caattactat cgggttgatg
    22261 aaagtctatg gcgaaatcaa ctgaagctgg tgcgtctgtc gaaatattct ctcgatgcag
    22321 cgctgaaaga gaaaagcacg cgcaataccc gggaaagact gaaagatttg cccaatatgt
    22381 cttttcatct ggaagcgatt ctcggggagg tg
//
LOCUS       DAEBXN010000063        22336 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.063, whole genome shotgun sequence.
ACCESSION   DAEBXN010000063 DAEBXN010000000
VERSION     DAEBXN010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22336
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.063"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..178
                     /gene="yjbD"
                     /locus_tag="KJL99_002506"
     CDS             <1..178
                     /gene="yjbD"
                     /locus_tag="KJL99_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HBD4437197.1"
                     /translation="VLTNSETNSIKKEYIDKLMVEREAEAKKARQLKKKQAYKPDPEA
                     SFSWSANTSTRGRR"
     gene            complement(311..1183)
                     /gene="rluF"
                     /locus_tag="KJL99_002507"
     CDS             complement(311..1183)
                     /gene="rluF"
                     /locus_tag="KJL99_002507"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HBD4437198.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            1531..2478
                     /locus_tag="KJL99_002508"
     CDS             1531..2478
                     /locus_tag="KJL99_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HBD4437199.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGMVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDIALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            2548..3354
                     /locus_tag="KJL99_002509"
     CDS             2548..3354
                     /locus_tag="KJL99_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBD4437200.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSTKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            3364..3771
                     /locus_tag="KJL99_002510"
     CDS             3364..3771
                     /locus_tag="KJL99_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HBD4437201.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVAFVPGENAGDIA
                     VKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            3771..4265
                     /locus_tag="KJL99_002511"
     CDS             3771..4265
                     /locus_tag="KJL99_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HBD4437202.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            4331..5128
                     /locus_tag="KJL99_002512"
     CDS             4331..5128
                     /locus_tag="KJL99_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HBD4437203.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALCWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWCKAEPQLQATASTALDQLDD"
     gene            5139..5927
                     /locus_tag="KJL99_002513"
     CDS             5139..5927
                     /locus_tag="KJL99_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBD4437204.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRLHLVFFNTTPAVCGPVIGAEIDDGTINGIKVGLMGPLAGVGDPL
                     VWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQLGFRKGVNIVSDM
                     GGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGSTVTMTVQNILDQL
                     CPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            5979..7208
                     /gene="sorE"
                     /locus_tag="KJL99_002514"
     CDS             5979..7208
                     /gene="sorE"
                     /locus_tag="KJL99_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HBD4437205.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVTTNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVDGEATHVVIPNEVMEQYCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGHTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEVEQYLLAHAEAISHD"
     gene            7590..8279
                     /gene="pepE"
                     /locus_tag="KJL99_002515"
     CDS             7590..8279
                     /gene="pepE"
                     /locus_tag="KJL99_002515"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HBD4437206.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(8370..10001)
                     /locus_tag="KJL99_002516"
     CDS             complement(8370..10001)
                     /locus_tag="KJL99_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HBD4437207.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(10221..13904)
                     /gene="metH"
                     /locus_tag="KJL99_002517"
     CDS             complement(10221..13904)
                     /gene="metH"
                     /locus_tag="KJL99_002517"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HBD4437208.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLA
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKADDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRIAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFAIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTCKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDHA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            14104..14928
                     /gene="iclR"
                     /locus_tag="KJL99_002518"
     CDS             14104..14928
                     /gene="iclR"
                     /locus_tag="KJL99_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HBD4437209.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            15245..17429
                     /gene="espL4"
                     /locus_tag="KJL99_002519"
                     /pseudo
     CDS             15245..17429
                     /gene="espL4"
                     /locus_tag="KJL99_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
     gene            complement(17398..19135)
                     /gene="aceK"
                     /locus_tag="KJL99_002520"
                     /pseudo
     CDS             complement(17398..19135)
                     /gene="aceK"
                     /locus_tag="KJL99_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
     gene            complement(19319..20623)
                     /gene="aceA"
                     /locus_tag="KJL99_002521"
     CDS             complement(19319..20623)
                     /gene="aceA"
                     /locus_tag="KJL99_002521"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HBD4437210.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINNLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSVGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(20653..22254)
                     /gene="aceB"
                     /locus_tag="KJL99_002522"
                     /pseudo
     CDS             complement(20653..22254)
                     /gene="aceB"
                     /locus_tag="KJL99_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
BASE COUNT         5477 a         5768 c         5633 g         5458 t
ORIGIN      
        1 cgtactgacc aactccgaaa ccaacagcat taagaaagag tacatcgata aattaatggt
       61 cgagcgtgag gctgaagcga aaaaagcccg ccaattgaag aaaaagcagg cttataaacc
      121 ggatcctgaa gcgtcattta gctggtcagc aaatacgtcg acgcgcggca ggcgctaatg
      181 aacaatcaac gcctgagatt ttcactcgtc tgtgtggaca caatcttccc ctcaccctaa
      241 ccctctcccc gaaggggcga ggggactgtc cgagctcttt ttggacgttt tcatcagtct
      301 gaggcgttaa tcagcgtccc ttctttttac gccccggcga ggtaaaacgc ttaccgttgg
      361 acgccgggcg accgcctttt tccgccgttt tttccatctt aacgactgga cgtttgatgc
      421 ccgctgtttt cggtttggcc ttcgctttag gttttgcctc ggaagaggaa ttttcgatga
      481 gcttaaagag gtcgattaac tcatcgtcgg ttaaatcgcg ccactccccc agcggaatgc
      541 cgcttaagct gacgttcata atgcgcgtac gttccagctt tttcacttca tagccgaaat
      601 gctcgcacat gcgccggatc tgacggttca gcccctgcac tagggtaatg cggaagacaa
      661 acggcgcttc ttttttgact ttgcactttt tcgtcaccgt cccgaggatc ggcacccccg
      721 cccccatacc acgaataaat tcatcggtaa tcggtttatc gaccgtcacc agatactctt
      781 tctcatgatc gttacccgca cggaggatct tattcaccag atcgccgtga ttggtgagga
      841 aaatcagccc ctgggagtct ttatccaggc ggccaatcgg gaacacgcgt ttgctgtggt
      901 taacgaaatc gacaatgtta tcgcgctcgc catcttcggt ggtgctgaca ataccgacgg
      961 gcttgttcag ggcgataagt accaaatctt cggcttcccg aggctcaatc aactgaccgt
     1021 ttactttcac aatgtcgccg ggtttcacct gatcgccaat ggtggctcgc ttgccattaa
     1081 ggaacacatt gccttgctcg atatagcgat ccgcttcgcg gcgtgagcaa attccgcttt
     1141 cgctgatgta tttatttaaa cggactgatg agtcgggcag cataaattct cctgtaaaac
     1201 gcggaatata ccgtacctct tagcggggaa aaacagggtt atgcggcaac gtcgaaatta
     1261 aatgtgatct gccacgcctt ttatcacaaa agtgtctccg gaagtacgat gcaaatttgc
     1321 ctggcaaaat aaggataatc aacatattac gaatcgctgc acacctgtgc tgatgactac
     1381 tacgcagaga aacaaatgtg caagagaaag cgctatttct ccccatgcaa agagtgaaaa
     1441 atcgctttga aaggccctca tgatggggtt atatcagatc aagcggtgag aaagcgtatt
     1501 tgcacaaatg tgcagaacca ggaggcggca atggaaaaca gtgacgatat ccgtttgatt
     1561 gtgaagattg cccaactcta ttacgaacag gatatgacgc aggcgcaaat cgcgcgcgaa
     1621 ctgggtattt accgcaccac catcagccgc ttgcttaaac gaggccgcga tcagggaatg
     1681 gtcaccatcg ccatcaacta tgactacaac gaaaatctct ggctggagca gcaactgaag
     1741 caaaagtttg gcctgaaaga cgttgtggtg gtgtcgggaa atgatgagga tgaagagact
     1801 caactggcga tgatggggtt acacggcgcg caactgctgg atcgcttgct ggagcctggc
     1861 gatattgtcg gtttttcctg gggtcgcgcg gtgagcgcac tggttgaaaa cttgccgcag
     1921 gcggggcaat cgcggcagtt aatctgcgta ccaattattg gcggaccgtc cggtaaactc
     1981 gaaagccgct atcatgtaaa cacattaacc tacagcgcgg cagcgaagct gaaaggggaa
     2041 tcgcatctcg cggattttcc ggctctgctg gataacccat taattcgtaa tgggatcatg
     2101 cagtctcagc actttaaaac catctctgcc tactgggata atctggatat cgccctggtg
     2161 ggaattggct caccggccat tcgcgacggc gctaactggc atgcgtttta tggtggtgaa
     2221 gagagtgacg acctgaatgc ccgccaggtt gctggcgata tttgctcgcg cttttttgat
     2281 attcacggcg caatggttga aacgaatatg agcgaaaaaa cgctctctat cgaaatgaat
     2341 aaattaaagc aggcgcgata ttccattggc attgccatga gtgaagaaaa atacagcgga
     2401 attattggtg cactgcgtgg aaaatatatt aattgtctgg taacgaatag cagcacagct
     2461 gaactattac tgaaataaaa atcttcccga tgtttaccgt aataaaaatt acggcgggtc
     2521 cctattatct aaaatcagga gtctgttatg caaacgtggt taaatttgca ggataaaatc
     2581 attattgtca ccggcggcgc atccggtatt ggtctggcga ttgtagagga attattagca
     2641 caaggcgcga atgtacagat ggtcgatatt cacggtggcg atggtcaata tgaaggtcat
     2701 aaaggctatc agttttggcc gaccgatatt tctagcacaa aagaggtaaa tcatacggta
     2761 gcggaaatta tccagcgttt tggtcgcatc gacggtctgg tcaataacgc cggggtcaat
     2821 ttcccgcgtc tgctggtcga tgagaaagcg cctgccgggc agtatgaact caacgaagct
     2881 gcattcgaaa aaatggtcaa tatcaaccag aaaggtgttt ttctgatgtc gcaggcggtg
     2941 gcgcgacaga tggtcaaaca acatgatggc gtgattgtga atgtttcctc agaaagtggg
     3001 ctggaaggct cagaaggaca aagctgttac gccgctacca aagccgcgct caatagcttc
     3061 acgcgctcct ggtcgaaaga gctgggtaaa cacggtatcc gtgtggtcgg tatcgcgccc
     3121 gggattctgg aaaaaaccgg gctacgcacg ccggaatatg aagaggcgct ggcgtggacg
     3181 cgcaatatca ccgtcgagca gctgcgtgaa ggctatacca aaaacgccat tcctattggg
     3241 cgcgccggaa gattagcaga agtagctgat tttgtttgtt atctgttgtc tgaacgcgcc
     3301 agctacatca ccggagtaac cactaacatt gcgggcggca aaacgcgcgg ctaaggaggc
     3361 tttatggtca acgccatttt ttgtgcccac ggcaaattag cttgcgccat gctggaatcg
     3421 gttcagatgg tttacggcaa cgccaatgtt gaagccgttg catttgtgcc cggtgaaaac
     3481 gccggagaca ttgcggtaaa gctggaaaag ttagtaagca ttcacaatca agatgagtgg
     3541 ctgattgccg tggatttaca gtgcggcagc ccgtggaatg cggcagcaat gcttgcgatg
     3601 cgcaatcctc gtttgcgggt aatcagcggc ctttctctgc cgctggcgct cgaactggtt
     3661 gataaccagg acagcatgaa tgttgatgaa ctgtgtgaac acctgacgca aatcgcaaag
     3721 caaacctgcg ttgtctggaa acaactggca acgacagagg aggatttctg atgaacatta
     3781 cgctcgcccg cattgatgac cgtctgatcc acggccaggt cactaccgtc tggtcgaaag
     3841 tggctaacgc ccagcgcatt attatctgca atgacgaagt ttataacgat gaagtgcgcc
     3901 ggacattatt gcgtcaggct gcgccaccgg gaatgaaggt taacgtggtc aatattgaaa
     3961 aagccgtcgc ggtttatcac aacccgcaat atcaggacga aaccgttttt tatcttttca
     4021 ctcgcccaca ggatgcctta gcgatggttc gccagggggt aaaaataggc accctgaata
     4081 ttggcggcat ggcctggcgg cccggtaaaa aacagttaac caaagcggtt tcattagatg
     4141 atgatgatat taacgcattt catgagttaa ataatcttgg tgtcatactg gatttacgcg
     4201 tcgtagcgtc agatccctca atcaatatta tcgacaaaat aaacgaacag ttaatcgcaa
     4261 attaaataat agcgcctgtg tacatatgct ttaacaggca gggatgacga cacttaaaag
     4321 gtgacaaatc atggaaataa gtaccctaca aatcattgct atatttcttt tttcctgtat
     4381 tgccggaatg ggcagcgtgc tggatgaatt tcagactcat cgcccgttaa ttgcctgtac
     4441 ggtgattggt ttaattctcg gtgatttaaa aaccggaata atactcggcg gtacgctgga
     4501 attgatagct ctctgctgga tgaacgtcgg cgcggcgcaa tctccggatt ctgcactcgc
     4561 cagcataatc tccgccattc tggttatcgt tggtcagcaa agcatcgcca ccggaatcgc
     4621 catcgcgttg cctgtggctg cggcaggcca ggtgctgacg gtgtttgccc gtaccatcac
     4681 cgtagtgttc cagcacgcgg cggataaagc agcagaagag gcgcggtttc gcactctcga
     4741 tattctgcat gtctccgcgc ttggcgtgca ggcgctgcgc gtcgccattc cggcactgat
     4801 tgtctcactg ttcgtcagcg ccgatatggt cagcaatatg ttaaacgcca ttcccgaatt
     4861 tgtgacccga ggactacaga ttgctggcgg ttttatcgtg gtggtcggtt acgccatggt
     4921 gctacgtatg atgggcgtga aatatttgat gcctttcttt ttcctcggtt tcctcgcagg
     4981 tggctacctc gatctcagcc tgctggcgtt cggtggcgtc ggcgtgatca tggccctggt
     5041 ctacatccag ctaaatccac agtggtgtaa agctgaacct caactccagg ccaccgcctc
     5101 caccgccctt gaccaacttg acgattaacg gagccatcat ggaacagaga aaaattacac
     5161 gcagcgatct ggtgagcatg tttctgcgct ccaacctgca acaggcgtcc tttaactttg
     5221 aacgtattca cgggctgggc ttttgctacg acatgatccc cgccatcaag cgactttacc
     5281 cattaaaaga ggatcaggtt gcggcgctca ggctacacct ggtgttcttc aataccacgc
     5341 cagccgtatg tggcccggtc atcggcgcgg aaattgatga cggtaccatc aacggcatca
     5401 aagtcggtct gatgggacca ttggcaggag ttggcgatcc actggtctgg ggaacgctgc
     5461 gcccgattac cgccgcgctc ggcgcatctc tggcactttc gggcaacatt ctcggcccgc
     5521 tgctgttctt ctttattttc aacgcggtgc gtctggcgat gaagtggtat ggcctacagc
     5581 tcggctttcg caaaggggtg aatatcgtca gcgatatggg cgggaatgtg ctgcaaaaac
     5641 tcaccgaagg cgcgtcgatt ctcggactgt ttgtgatggg cgtgctggtg accaaatgga
     5701 cgtcaatcaa cgtaccgttg gtggtttcac aaacgcatgc cgccgatggc tccaccgtca
     5761 ccatgaccgt gcagaacatt ctcgaccaac tttgccctgg tttgctggcg ctcggtctga
     5821 cgctactaat ggttcgtctg ctcaacaaaa aaattaaccc ggtatggctg attttcgccc
     5881 tgtttggctt agggattatc ggcaatgctc tgggcttcct gtcctgattc ttcgccccgg
     5941 cacgactgcc gaggccatcg ctcaacatga ggtggtttat gaaaacaaca gctctgcgtc
     6001 tttatggtaa acgtgattta cgcctggaaa cctttgacct tcctgaaatg caggaggatg
     6061 aaatcctcgc aacggtggtc actgacagcc tgtgcctctc ttcctggaaa gaggccaatc
     6121 tgggtgaaaa ccataaaaaa gtacccgacg atgtgacgac caacccaatc atcatcggcc
     6181 acgaattttg cggcgatatt ctggccgtgg gtaaaaagtg gcagcacaaa ttccagccgg
     6241 gtcagcgtta tgtgattcag gccaacctgc aactccccga ccgcccggat tgccccggct
     6301 actccttccc gtgggtagac ggcgaagcga cgcatgtggt tattcccaac gaggtcatgg
     6361 aacaatattg cctgctggca tacgacggcg aaacctattt tgaaggctca ctggttgaac
     6421 cgctttcctg cgtgattggc gcgttcaacg ccaactatca tcttcaggaa ggtagttata
     6481 accacacgat ggggattcgc ccgcaagggc acacgctgat cctcggcggc accggaccaa
     6541 tgggactgtt ggcgattgat tatgcgctgc atggaccagt taacccgtcg ctgctcgtca
     6601 ttaccgatac cgacaacgac aaattgagtt atgcgcgcaa gcactatcca tcagaaccgc
     6661 aaacactgat tcattatctc aatgccaccg atgcagcatt tgatacgtta atggcgctga
     6721 gtggcggtca cggcttcgat gatattttcg tctttgtgcc taatgaagga ctggtgactc
     6781 tcgcctcttc cttgctggcg acagatggtt gcctgaattt cttcgccgga ccgcaggata
     6841 aacatttcag cgcgccaatt aatttctacg atgtgcatta tgcatttacc cactacgtgg
     6901 gcacgtcagg cggcaatacc gacgacatgc gcgcggcggt caaactgatc gaagagaaaa
     6961 aagtgcaggc cgcaaaagtg gtaacacata ttcttgggct gaatgccgcg ggcgaaacca
     7021 cgcttgaatt gcctgccgtc ggcggcggca aaaagctggt gtataccggg aaatacctgc
     7081 cgctgacgtc actcacgcag attcaggatc aagcactggc ggcgattctg gcgcgtcatc
     7141 aggggatctg gtccggtgag gtggagcaat acctgctcgc ccatgcagag gcaatttccc
     7201 atgattaatc gcgctactta actctgcatg tcgctggctg gtcgccccag caacttcgcg
     7261 accggatgtg cggctgattg ctgaagcagc acaatttgca cgagaaggat aaaaaatatc
     7321 agcgccttaa ggggcgctca aattggtggc aaagtacaca ttgtttatgc cggatgcgga
     7381 atgaagcctt attcggtcta cataattttg aaaatccaat atattgcagt tatctggtag
     7441 gccaggtaag cgtagcgcat caggcaatgc tgtgtttgtc atcagtctca gcgcccggtg
     7501 caatacgtga catcgtgagc catgtcagta agtggttaaa ttcgcagtgg tagcatgcct
     7561 tccgtgattt cttactaacc ggagaagtaa tggaactgct tttattgagt aactcgacgc
     7621 tgccgggtaa agcctggctg gaacatgcac tgccgctaat tgctgaacag ttgcagggtc
     7681 gccgctcagc ggtgtttatc cctttcgctg gcgtaacgca gacctgggat gattacacag
     7741 agaaaacggc tgcggttctc gctccgctgg gtgtttctgt caccggtatt catagcgttg
     7801 tcgatcccgt tgccgcgatt gaaaatgcgg aaattgtgat tgtcggcggc ggtaatactt
     7861 tccagttgct gaaacagtgc cgcgagcgcg ggctgctggc tccaattact gacgtggtta
     7921 aacgtggcgc gctgtatatt ggctggagcg caggcgctaa ccttgcttgc ccaaccattc
     7981 gtaccaccaa cgatatgccg attgtcgatc cgcaaggttt cgatgcgcta aatctgttcc
     8041 cgttgcaaat caacccgcac ttcaccaacg cgctgccgga aggccataaa ggtgaaaccc
     8101 gtgagcagcg tattcgcgaa ctgctggtcg tcgcgccaga actgacgatt attggtctgc
     8161 cggaaggtaa ctggatcaca gtgagtaaag gtcacgctac gctgggtggc ccgaacacca
     8221 cttatgtgtt taaggctggt gaagaagcgg ttccgctgga agctggtcac cgtttttaag
     8281 tgaattgccg gatgcatgat ccggcatctt gttcatagtg cctgatgcga cgcttgacgc
     8341 gtcttatcag gcctacagga ttcacaatat taatactcgt cccgtccttc atcttcatcc
     8401 ggctgttcca gcacactgta agccaccgaa caaaacagcg agttcaggcg ctgcatatct
     8461 cccagtaagc ctaaatgtag cgaactggtt tcaatgctct gcacgttttg ctgatgcagg
     8521 cgatcgacgt gggcgtggga atagcggcga ttaagaatgc gaaaacgatg tttgctgcga
     8581 cgcaaacgac gagcgctggt gacatcgcca gagaagaaca ccgacattgc cagctttaaa
     8641 ttactgagca attgctcata gagcgcatcc agttccttca acccatcaag cgaaaatgcc
     8701 cgccgtgctg ccagcgattt atcggcaatt tcgctgccca tgcgctcgac gatatcggag
     8761 gcctgttcaa ggttgagcga catttcgatg atctccgccc agcggcgcga ctcttcctcc
     8821 gccaactctt cttttggcat ccgcgccaga tacagcttaa tggcggtgta gagaacgttg
     8881 atatcatctg ccagcttacg cagctctttc tcctgccgtg gctcgccgtg catcacttta
     8941 ttcagccctt ccatcatctg ttccatggcg tcgccaatgc gcagggtttc gcgcgcggcg
     9001 ttcgccagag caagcgtggg cgtgtccagc gcgctgacat ccagatgttt aggccgtagc
     9061 tgggtatcca gttccggttc atcgcgaata atcgttttgc aaaaccgtgc catcgggtca
     9121 acaaatggca gcatgaccag gcaacgtaca aggttgtaga agacgtggaa atagatcacc
     9181 agttccgctt ttggcaatga cagcttcccc attgtctctg ccagcaaatg gacaaacggc
     9241 aggataatca ggctacccac cagcttaaac agcagactac ccagcgcgac acggcgggct
     9301 gcggcattgg cggcactgtt gttgagcatc gccagcaggc cggaaccgag gttagctcca
     9361 atcaccagac agagcgccac ggggaaggag ataatgcctg cagcggtcag agtcgcggtc
     9421 agcagtacag cagcaaggct ggagtagctg ataatggcga acatcgcgcc aatcagcgca
     9481 tccagcagaa tatcgccggt cagcgaggca aagatcacct gaacgccgtt tgcctgggtg
     9541 atcggcgtta cggcctgcac aatcaactcc agcgccagca aaatcagccc aagaccaata
     9601 ccgacgcggc ccagttgccc ggcgcgtgac tgtttgcgtc cgaggaaaaa aatcacgccg
     9661 ataaaaataa gtaacggtga cagccaggat aagtcgaagg tgagaatacg cgccattagc
     9721 gccgtcccga catctgcccc cagcacaatg accagagccg gtgcgagggc taccagatcc
     9781 tgagcgacga acgaggtcac cagcatggtg gtggcattac tgctctgtac cagtgcggta
     9841 acgccgatcc ccgcgcaaaa ggcgagcggc tttttttcga cgctccggct aaggacagta
     9901 cgcaaacgcg cgccgaagac gcgcattacg ccggttcgaa caatatgagt cccccagacc
     9961 agcagggcga cggcagaaag caggtgaagc agcgttaaca cgagttcaga ttctccttat
    10021 cgttatatgt tcctgaagca attgaatgac caaaggaccg gaaacacgct gactggcatc
    10081 tttactgtgc cttttgacat tcgtttttcc ggctaagatt attccagcct gtttccagta
    10141 taagtgtttc atattaataa agagacaggg cactcattga gcgccctgtt tgttgtaagg
    10201 aaaagtgaca gatttgtgaa tcagtccgcg tcatacccca gattcggtgc cagccagcgc
    10261 tcaacatcgg agacgctcat acctttacgg cgggcatggt cttcaacctg atcgcgctga
    10321 atttgcgcca ccgcgtagta cttgctgtcc ggatgactga aataccaacc cgaaaccgat
    10381 gcaccaggcc acatcgcgaa agattctgtg agtttcatgc cagtatgttt ttccacttcc
    10441 agcagctccc agatggtggc tttttccgta tgttccgggc aggccggata gcccggtgcc
    10501 ggacggatgc cctggtagtt ttcgcggatc agttcttcgt tgctgaggtt ctcgttcggc
    10561 gcatagcccc agtagacttt acgcacacgc tcatggagat actccgcaaa ggcttcggct
    10621 aaacggtcgg caagcgcttt caccatgatt ttgttgtaat catcgtgctg cgcttcaaag
    10681 gcatcagcca gtgcgtcctc ttccagcccg ccagtcacgg caaatgcgcc gatgtaatct
    10741 gctttaccag aaagcttcgg cgcaacgaag tcagcgagac agtagttagc gaatcccgtt
    10801 ttttcggtct gttgacgcag atggtggctg acgttgatca catgggtgcg cgtttcgtca
    10861 cggtagattt caatgtcatc gcccacacgg tttgccggga acaggcccac cacgccacgc
    10921 ggattcagcg ttttctcggc gcttaattta tccagcatgt cgttggcgtc tttaaacagc
    10981 cgctgcgcct caacgcccac cacttcatct tccagaatgc gcggatactt cccggccagc
    11041 gaccaggtca taaagaacgg tgtccagtcg atgtaattac gcagcgtttc gatgctggct
    11101 tccacttcct gcacgccgag acggtgcgcc accggcggcg tgtaagcctg ccagtcaaat
    11161 gcgaaatcgt tatcgcgcgc cgcttccagc gtgaccggtg gtgtgcgtgg tttcttgcgc
    11221 ccgtgctgaa tacgcacggt ttcgtactcc ttgcaggtac gagcgacaaa atcatcacgc
    11281 tgagtatcgg aaagcagcgc cgctaccaca ccaacggtgc gcgaggcatt ctgcacatac
    11341 accgtagggc cgctgtagtt ctgctcgatt ttcaccgccg tgtgcgcttt tgaggtggtc
    11401 gcgccgccaa tcagtaacgg aatggcgaag ccctgacgct ccatctcttt cgccacgtta
    11461 accatctcgt ccagcgacgg cgtgataagc cccgaaaggc caatcaaatc agcattcact
    11521 tctttggcga tacggagaat tttttccgca ggcaccataa cgccgagatc gacaatttcg
    11581 tagttgttac attgcagcac cacaccaacg atatttttac cgatgtcgtg gacatcgcct
    11641 ttcacggtgg cgatcaccat cttgccgttg gttttaccct gctctttgct ggcttcaata
    11701 aacggttcga gatacgccac tgcctgtttc atgacgcgcg ccgatttgac cacctgtggc
    11761 aggaacattt tgccttcgcc aaacagatcg ccgaccacgt tcatgccgtc catcaacggg
    11821 ccttcaatca cttcaatcgg gcgcgtagcc tgctggcggg cttcttcagt atcctgctcg
    11881 ataaactcgg ttatgccttt gaccagcgag tattccagac gtttattcac gtcccacgag
    11941 cgccactccg cttgctgggc gttagcagtg tcgtccgctt tactgccgcg atatttctcg
    12001 gcaagctcca gtaaacgctc agtgccatcg tcacgacgat taagaatcac atcttccacc
    12061 gcgtcgcgca gttcagcggg taggtcgtcg taaatcgcca gttgcccggc gttgacgatc
    12121 cccatatcca tgccattgcg aatagcgtag tagaggaaca ctgcgtgaat ggcttcgcgc
    12181 accggatcgt tgccacggaa tgagaaagaa acgttagata cgccgccgga aatcagcgcg
    12241 tgcggcagtt cgcgtttgat gtcttcacac gcgccgataa agtcctgcgc gtagttgttg
    12301 tgctcttcaa taccggtggc gaccgcgaag atgttcgggt cgaagatgat atcttctggc
    12361 gggaagccaa cctcttcggt gaggattttg tacgcccgac ggcaaatctc gattttccgt
    12421 gcgcgagtat cggcctgtcc ctgttcgtca aaggccatca ccaccactgc cgcaccgtag
    12481 cgacgcaaca atttcgcgtg atggataaag gcgtcgacgc cctctttcat cgagatagag
    12541 ttaacaatgc ctttgccctg gatacacttc agaccttttt caatgacatc ccattttgag
    12601 gagtcgatca taatcggcac gcgagcgata tccggttcac cggcaatcag attgagaaaa
    12661 cgcaccatcg ccgcttcggc atcgagcatc ccttcatcca tgttgatatc gataatctgc
    12721 gcgccgtttt ccacctgctg acgcgccaca tccagcgcct cgctgtattt ctcttctttg
    12781 atcaggcgct tgaacttagc agagccggtg acgttggtgc gttcacccac gttcacaaac
    12841 aggctatcgt cgccaatgtt cagcggctcc aggccagaca aacggcaggc tacgggaatt
    12901 tccggcagtt tgcgcggcgc taatccttct actgcacgac tcatcgctgc aatatgttgc
    12961 ggcgtggtcc cgcagcaacc accgacgata ttgagaaagc ccgcttgcgc ccattcacgt
    13021 atctgttttg ccatcgtgtc ggcgtcgaga tcgtattcac caaaggcgtt aggcagcccg
    13081 gcgttcgggt gggcggtgac gtagcattcc gcaatccgtg atagttcctg cacgtactgg
    13141 cgcagttcat cgggccccag cgcacagttc aggccaaagg tcagcgcttc ggcgtggcgc
    13201 aatgagttgt aaaatgcttc ggtggtctgc ccggagagcg tgcgcccgga ggcgtcggtg
    13261 atggtgccgg agatcataat cggcagctca acgcccagcg cttcaaactc cgttttcacc
    13321 gcaaagaccg ccgctttggc gttaagggtg tcgaaaacgg tttcaatcag gatcagatcc
    13381 gcaccacctt ccaccagcgc tttggtggat tcgcgatagg ccgccgccag ctggtcaaaa
    13441 gtgatgttgc ggaatgccgg atcgttgacg tccggagaaa tagacgccgt gcggttggtt
    13501 gggccaagaa caccggcaac gtagcgcggt ttctccggcg tgcgcgcggt ccactcgtca
    13561 gcacaggcgc gcgccagttt cgccgccgca aagttgattt ccgccgacag ggattccatc
    13621 tggtaatccg ccatcgcaat ggtcgtggag ttgaaggtgt tggtttcgat gatatccgcg
    13681 cccgcttcaa agtaggcgtt gtggatagcg gcgatcactt ccggtttact gagtaccagc
    13741 aggtcgttgt tgcctttgag gtcgcatggc cagtcggcaa agcgttcacc acgaaaatcg
    13801 gcttcgttca gtcgatagct ctggatcatg gtgcccatac cgccgtccaa caccagaata
    13861 cgttcattta actgcgcacg cagttgttcc actttgctgc tcacacttgc tcccgacacg
    13921 cttaacccag atttaataaa aattcaacaa accatactgg aataaacgca tctgtggtaa
    13981 aagcgaccac cacgcaacat gagatttgtt caacattaac tcatcggatc agttcagcaa
    14041 ctattgcatt agctaacaat aaaaatgaaa ataatttcca cgatacagaa aaaggaggct
    14101 gtcatggtcg cacccattcc cgcgaaacgc ggcagaaaac ccgccgttgc caccgcacca
    14161 gcgactggac aggttcagtc tttaacgcgt ggcctgaaat tactggagtg gattgccgaa
    14221 tccaatggca gcgtggcact cacagagctg gcgcaacaag ccgggttacc caattccacg
    14281 acccaccgcc tgctaaccac gatgcaacag cagggtttcg tgcgtcaggt cggcgaactg
    14341 ggacactggg caatcggcgc acatgccttt atggtcggca gcagctttct ccagagccgt
    14401 aatttgttag caattgttca ccctatcctg cgcaatctaa tggaagagtc tggcgaaacg
    14461 gtcaatatgg cggtgcttga tcaaagcgat cacgaagcga ttattatcga ccaggtacag
    14521 tgtacccacc tgatgcgaat gtccgcgcct atcggcggta aattgccgat gcacgcttcc
    14581 ggtgcgggta aagccttttt agcccaactg agcgaagaac aggtgacgaa gctgctgcac
    14641 cgcaaagggt tacatgccta tacccacgca actctggtgt ctcctgtgca tttaaaagaa
    14701 gatctcgccc aaacgcgcaa acggggttat tcatttgacg atgaggaaca tgcgctgggg
    14761 ctacgttgcc ttgcagcgtg tattttcgat gagcaccgcg aaccgtttgc cgcaatttct
    14821 atttccggac cgatttcacg tattaccgat gatcgcgtga ccgagtttgg cgcgatggtg
    14881 attaaagcgg cgaaggaagt gacgctggcg tacggtggaa tgcgctgact ttttctggcg
    14941 ggcagaggca atattctgcc catcatacct gagtggcaat agaataaggg tgtctgttaa
    15001 tcgcattgac gccaaaataa cttaatgtca tacacttcac acacttttat taactcagca
    15061 ttatttttaa acatcaaacc acttaattat taacaaaatc gcaagatctc attccaccga
    15121 aacttttacg gctgcattat gagattgagc gatttatgat atttttttag ttgtaatcat
    15181 attaaatttt ttcactgttc tgatagttaa aattcaagac atcaataaac aatgagatat
    15241 ttaaatgatt actcgtattc ctcgtagttc tttctctgca aatattaata atacagccca
    15301 gacaaatgaa caccaaaccc tgagtgaatt gttttataaa gaactcgagg ataaattttc
    15361 tggcaaggag ctggcgacgc ctctattaaa aagcttctca gagaactgta gacaaaatgt
    15421 tcgtcatatc tttagcaaca aggattttgt cattaaattt tccacgtccg tcttacaagc
    15481 tgataagaaa gaaattacga tgattaataa aaacgaaaac acgacactca ctcaaaccat
    15541 tgctacaata tttgaagaat acctaatgga aattttacct caacgctcag acactcttga
    15601 taaacaagaa ttaaaccaaa aatcagatag aaaagaaaaa gaattcccaa gaattaaact
    15661 taatggtcaa tgttatttcc cggggcgccc caaaaccgta tagtataccg acacattgct
    15721 gcacaatata ttaatgatat ttatcagaat gttgattaca aaccccatca agatgattac
    15781 tcttcagctg aaaaatttct cactcacttc aacaaaaaat gcaaaaacca gactttggcg
    15841 ttgatttcca gccgtcctga ggggcgttgc gttgctgcct gcggtgattt cgggctagtt
    15901 atgaaagcat attttgacaa gatggaatca aatggcatca gtgttatggc agccatatta
    15961 ctggtggata accatgcttt gacggtccgg ctaagaataa agaacacaac tgaaggatgt
    16021 acccattacg tggtttcggt ttatgatcct aatgtaacta acgataaaat aagaattatg
    16081 agcgaaagca aagaggatat taaacactat tctctgatgg attttatgaa tgtagattat
    16141 agcctcctga aatggtcaaa tgatcatgtt attaaccaat ctgttgcaat aattccagca
    16201 cttccgaaag aacagctatt gatgttaaaa ggaactgtgg atgaaataac ccctccatta
    16261 tcaccagcaa cgatgaattt gctaatggca attggtcaga atcaccaact tacgcaactg
    16321 atgattcagc tccagaaaat gccagaacta catagaacag aaatgttgac tgcctataat
    16381 agtattaacc tccccggttt atatttggct ataaattatg gcaatgcgga tatcgttgag
    16441 actattttca attcattgtc agaaacagga tatgaagggt tactctcgaa aaaaaatctc
    16501 atgcatattc tggaggcaaa agataaaaat ggtttttctg gattattttt agcgatatca
    16561 cgtaaggata aaaatgttgt aacctcgatt ctgaacgtct tacctaaact ggccgcaaca
    16621 catcatttag ataatgaaca ggtgtataaa ttcctgagtg ccaaaaatag aacgtccagc
    16681 catgttttat accatgttat ggcgaatggt gatgccgaca tgctgaaaat ttttttggtc
    16741 gcgttacctt tgttaattcg cacatgtcat ttgactaaag aacaggtact cgatctcctg
    16801 aaggcaaagg atttttatgg ttgcccaaga ctatacctgg cgatgcaaaa tggacatagc
    16861 gatatcgtga aagttattct cgaagcattg ccctgtctgg cccaggaaat taacatttca
    16921 gcttccgata ttgtagatct tctgaccgct aaaagtcttg cgcgcgacac gggtttgttt
    16981 atggccatgc agcgcggaca tatgaacgtt attaatacta tttttaacgc attacccact
    17041 ctttttaata cgtttaaatt cgataaaaaa atatgaagcc cctcctcctg gcaaataatt
    17101 ctaatgaata tcccggtttg ttttcagcga tacagcataa acaacaaaac gttgtagaga
    17161 cggtttatct tgctttatct gaccatgcac gcctgtttgg atttaccgct gaagatatta
    17221 tggatttttg gcaacacaaa gcgccacaaa aatactctgc ctttgagttg gcttttgaat
    17281 ttggtcaccg ggttattgct gaattaatcc ttaatacatt aaataagatg gctgaaagct
    17341 ttggctttac ggataaccct cgatacattg cggagaaaaa ttatatggaa gctttactca
    17401 aaaaagcatc tccccatacc gtacgctaaa tctttgcctg cgccgatacg cataaacatc
    17461 ttccacatgc ccttcacgta tgcggttttg tagtgcgcgc cagtaatcag cgcggaacag
    17521 gtcggcgtgc atctcttcaa acagcggacc aatacgcggg tcggcgcata gccagtggcg
    17581 aaactcttcc gggaaaacat cgcccggcga gacgctgtac cacggttcgc tggcaagttc
    17641 gtcttccgga tagcgcggcg gcgggatgtc gcggaaattc acttccgtca tgtagcaaat
    17701 ttcatcgtaa tcataaaaaa ccacacgccc gtgacgggtg acaccgaagt ttttaaacag
    17761 catgtcgcca gggaaaatgt tagcagcggc aagctggcga atagcgttac cgtattcttc
    17821 aatggcgtcg cgcaactgct gaccttccac ttgttccagc cagatattga gcggcaccat
    17881 ccgccgctcg attatagaga tggcgaatta caatctgctc accaagatcg gtgatttttt
    17941 ccgctgcttc ctggagcagt aattccatta ataccgggga aatatgccgc ttctccagca
    18001 caaagttttc aaactcctgg gtgtccgcca ttcggcccac acggtcgtgc tctttcacca
    18061 gttgatagca ggcacgaacg tgagcggcag acatctcttt ctgcggcgcg aacttgtctt
    18121 tgatgacttt gaatacccga tcaaagcccg gcagcgtaaa caccaacatc accattccac
    18181 gaatacccgg cgcttcaatg aactgctcat tacagccctg tagataaacg agatattcgc
    18241 ggtagctttc ggttttggcg tgcttctggc agccgatagc catatacaat tcagcagtgg
    18301 ttttacctgg cagaatttcc cgtagccact cgaccagcgc tgcgggcagc ggtgcataaa
    18361 ccataaaata agaacgcgca aagccaaaaa caatgctcgc ttcggcggtc gtcgtcaggc
    18421 aggtatcaat aaataactcg ccgtcgtccg tctggtggat cggcagtaag aagggcaacg
    18481 tgccggaagg cgtgatcagt ttgcccacca gccaggcggc tttattgcgg taaaacagtt
    18541 cgttcgccac ctgtaaatga ctttccgcga ggttgtctgt ccccagcgtt tccgtcagat
    18601 ggcgaatgat gtaatgaatg tcacggcttt tattctgcca gcgcaggcgc agcggcaggt
    18661 cgctgataac gcgcatcagt agagattccc agccgtgatc ggggtgaaag tctttcgcca
    18721 gcgggcgggg aatggtacga aagcggcgct ctggctgaga gctaaagata aaaagccgct
    18781 cgggagtaag cgagcggtgg tcaaataacc gacagtacac ggagttaaaa aagctctccg
    18841 caatctcgaa gcgcgggtaa tccggcaaca gccgcgtgta atgctcttta acgcgcagta
    18901 aaaatgccgc gtccgtactt tggccgttag taatgcagcg cagttgctcc acgaccagac
    18961 caacgtgatg atcgtaaaga tggatacggt ttttcatcgc ctgctggaca gcatgccagt
    19021 cggcctgttc gaaacgctgc tgcgccccgg aagtcacttc gaggaatcga ccatactgag
    19081 cgtcgaagcc ttgcaaaatg gtttgagcaa tcagtaattc caggccacgc ggcatttagc
    19141 gccctcatca ggagcagaga attgccggat gcggcgtaaa cgccttatcc agcctacgtt
    19201 cggcaacggt tgtaggcatg ataagacgcg caagcgtcgc atcaggcatc gtgcaccgat
    19261 tgccggatgc ggcgtaaatg ccttatccgg cctacagtca gcaacgtttg taggttgctt
    19321 agaactgcga ttcttcagtg gagccagtca gcgcggtgac tgaagacgtg ccgccctgaa
    19381 taatagtcgt cactttatcg aagtaacctg tacccacttc ctgctggtga gatacgaagg
    19441 tatagccatc tttcgcggcg gcaaattccg gctgctgcac tttctcaaca tagtgcttca
    19501 taccttcgcc ctgcgcataa gcgtttgcca ggtcgaacat gttaaaccac atgctgtgga
    19561 tacctgctag ggtgatgaac tggaacttgt agcccatatc cgacagctgc tgctggaagc
    19621 tggcaatagt tttgtcgtcg aggtttttct gccagttgaa cgacggcgag cagttataag
    19681 ccagcagttt gcccggatat ttcgcgtgga tagcttgtgc aaagcgacgc gccagttcca
    19741 gatccggcgt ggaggtttca caccagacca ggtcagcata tggcgcatac gccaggccac
    19801 ggctgatcgc ttgctcaatg cccgcatgag tacggaagaa gccttcactg gtacgctcgc
    19861 cggtaataaa ttcgctgtca tacgggtcgc aatctgaggt gatcagatcc gccgcatcag
    19921 catcggtacg tgcaaccagc agggttggaa cgcccgtcac gtcagctgcc agacgcgccg
    19981 cgaccagttt ctgaatagct tcctgagttg gcactaaaac tttgccgccc atgtgaccgc
    20041 atttcttcac tgacgccagc tgatcttcga agtgaactgc cgcagcaccg gcttcaatca
    20101 tcgctttcat cagttcaaag gcattcagga caccgccaaa accggcttcc gcatcggcaa
    20161 cgatcggcag gaagtaatcg acatagcgcg gatcgcccgg ctcaatgccc acggaccatt
    20221 ggatctgatc ggcacgacgg aaggtgttgt tgatccgctc caccacagct ggcaccgagt
    20281 ttgccggata gagcgactga tccggataca tgctggccgc caggttagcg tccgccgcta
    20341 cctgccatcc cgacagatag actgcttcaa tacccgcttt cgcctgttgc agcgcctgac
    20401 cgccagtcag tgcaccgagg ttgttgatgt agcctttttt cgactcaccg tgcagcagac
    20461 gccacatttt cgctgcgccc agttgcgcca gcgtgcattc aggattgact gaaccgcgta
    20521 atttcaccac atcttccgca ctgtatgggc gagtaatgcc ttcccaacgc ggttgagtcc
    20581 actctttctg taattcttca atttgttgtg tacgggtttt catgtgcaga tgctccatag
    20641 ttatgtggtg gtttacgcta acaggcggta gcctggcagg gtcaggaaat caattaactc
    20701 atcggaagtg gtgatctgtt ccatcaagcg tgcggcatcg tcaaaacggc cctgggagaa
    20761 acgttcttcg cccagttcgc tggcaatgac tttcatctct tcgcccagca tctggcggaa
    20821 caaggctttg gtcaccggtt tgccattgct caacgttttt tgatgatgga tccactgcca
    20881 gatcgaggta cgggaaattt cagccgtcgc cgcatcttcc atcaggccat aaatcggcac
    20941 gcagccgttg ccggagatcc acgcttcgat gtactgcaca gccacgcgaa tgttagcgcg
    21001 cataccttct tcggtgcgtt caccatcaca aggtgccagc agctgatcgg cagtaatcgg
    21061 cgcgtcttgt tcgcgcaata cttcaagctg atttttacgg gagccgagaa tgtcgttgaa
    21121 taccgccatt gccgtgtccg caaggcctgg gtgagcgatc catgtgccat cgtgaccgtt
    21181 attggcttcc agcgctttat ccgcttttac tttgttgagc acctggttat tacgctcttc
    21241 atctttgctc ggaataaacg cggccatgcc gcccatcgca aaagcaccgc gtttatggca
    21301 ggttttaatc agcaggcgtg agtaagcatt caggaatggt ttatccatcg tcacggcctg
    21361 tctgtctggc aggacacgat cgggatagtt tttcaacgtt ttgatatagc tgaagatgta
    21421 atcccagcga ccgcagttca gaccaacaat atggtcgcgc agcgcgtgaa ggatttcgtc
    21481 catctggaac acggcgggca gcgtttcaat cagcaacgtc gccttgatgg tgccgcgcgg
    21541 cagattaaag cgatcttctg catagctgaa gacttcgctc caccaggccg cttcctgcta
    21601 ggactgggtt ttcggcagat agaaataggg accgctgccc tttgccaaca gtgcctgata
    21661 gttgtggaag aaatagagcg caaaatcaaa caggctaccg gggattgcct caccacgcca
    21721 ggtgacatgt ttttccggca agtgcagacc gcgtacccga caaatcaaaa ccgctggatt
    21781 gggcttgagc tgataaattt tgcctgcttc attggtatag ctgatggtac cgttaaccgc
    21841 atcgcgcagg ttaatttgcc cgtcgatcac tttgttccag tctggtgcca gtgaatcttc
    21901 gaaatcggcc ataaagactt tcacattggc gttcagtgcg ttgatcacca tcttgcgctc
    21961 taccgggcca gttatctcta cgcggcggtc ttctaagtcc gcaggaatcc cgcgaatttt
    22021 ccaatcagca tcgcgaatgg aagccgtttc cgaaataaaa tcaggcaacg ttccgttatc
    22081 aatatcttgc tgctgctgaa tgcgcgctgc cagaagttta ttgcgttgtg gcgtaaaatg
    22141 cgtcaccagc tcagtcagaa attctaccgc ttcggcagta agaatttgct tctcctgctc
    22201 gccatacggc cttgtgaaag ccagttcatc ggttgttgtt gcctgttcag tcatcgtgca
    22261 gctcctcgtc atggatccga aaacttcccc tctgtgaacg aaggaacgtt gtgcgctttt
    22321 cgttcagcac aactaa
//
LOCUS       DAEBXN010000064        22314 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.064, whole genome shotgun sequence.
ACCESSION   DAEBXN010000064 DAEBXN010000000
VERSION     DAEBXN010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22314
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.064"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(<1..1025)
                     /locus_tag="KJL99_002523"
     CDS             complement(<1..1025)
                     /locus_tag="KJL99_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD4437211.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEA"
     gene            complement(1127..2107)
                     /locus_tag="KJL99_002524"
     CDS             complement(1127..2107)
                     /locus_tag="KJL99_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HBD4437212.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGATSVRFYPCYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYASAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(2115..2825)
                     /locus_tag="KJL99_002525"
     CDS             complement(2115..2825)
                     /locus_tag="KJL99_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HBD4437213.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(3042..4865)
                     /gene="typA"
                     /locus_tag="KJL99_002526"
     CDS             complement(3042..4865)
                     /gene="typA"
                     /locus_tag="KJL99_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HBD4437214.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            5238..6647
                     /gene="glnA"
                     /locus_tag="KJL99_002527"
     CDS             5238..6647
                     /gene="glnA"
                     /locus_tag="KJL99_002527"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HBD4437215.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            6820..7869
                     /gene="glnL"
                     /locus_tag="KJL99_002528"
     CDS             6820..7869
                     /gene="glnL"
                     /locus_tag="KJL99_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HBD4437216.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            7881..9290
                     /gene="glnG"
                     /locus_tag="KJL99_002529"
     CDS             7881..9290
                     /gene="glnG"
                     /locus_tag="KJL99_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HBD4437217.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAQAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRTKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            9402..9512
                     /locus_tag="KJL99_002530"
     CDS             9402..9512
                     /locus_tag="KJL99_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HBD4437218.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(9741..11114)
                     /gene="hemN"
                     /locus_tag="KJL99_002531"
     CDS             complement(9741..11114)
                     /gene="hemN"
                     /locus_tag="KJL99_002531"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HBD4437219.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFHFNTDAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVYEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPLEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(11303..11812)
                     /gene="yihI"
                     /locus_tag="KJL99_002532"
     CDS             complement(11303..11812)
                     /gene="yihI"
                     /locus_tag="KJL99_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HBD4437220.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            12394..13026
                     /locus_tag="KJL99_002533"
     CDS             12394..13026
                     /locus_tag="KJL99_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HBD4437221.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKALLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(13408..16194)
                     /gene="polA"
                     /locus_tag="KJL99_002534"
     CDS             complement(13408..16194)
                     /gene="polA"
                     /locus_tag="KJL99_002534"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HBD4437222.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEVGKWLQAKGAKTAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISHERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKELGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            16558..17490
                     /locus_tag="KJL99_002535"
     CDS             16558..17490
                     /locus_tag="KJL99_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HBD4437223.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGQDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(17531..18961)
                     /locus_tag="KJL99_002536"
     CDS             complement(17531..18961)
                     /locus_tag="KJL99_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HBD4437224.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(19116..19742)
                     /gene="dsbA"
                     /locus_tag="KJL99_002537"
     CDS             complement(19116..19742)
                     /gene="dsbA"
                     /locus_tag="KJL99_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HBD4437225.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEEK"
     gene            complement(19759..20745)
                     /gene="srkA"
                     /locus_tag="KJL99_002538"
     CDS             complement(19759..20745)
                     /gene="srkA"
                     /locus_tag="KJL99_002538"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HBD4437226.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFKQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAQQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRHWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(20822..21091)
                     /locus_tag="KJL99_002539"
     CDS             complement(20822..21091)
                     /locus_tag="KJL99_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HBD4437227.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDFNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            21161..21745
                     /gene="mobA"
                     /locus_tag="KJL99_002540"
     CDS             21161..21745
                     /gene="mobA"
                     /locus_tag="KJL99_002540"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HBD4437228.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALK
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            21727..22254
                     /gene="mobB"
                     /locus_tag="KJL99_002541"
     CDS             21727..22254
                     /gene="mobB"
                     /locus_tag="KJL99_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HBD4437229.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAVQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         5519 a         5597 c         5341 g         5857 t
ORIGIN      
        1 gcttcgccga taggtgcaaa aaatacggct cgctgtgtaa agactatcgc gccaaagccc
       61 agcgtacatg ccatccctaa atagaccggc atactttcgt gcggcagcat aatcaacagt
      121 acgagcgcag cggtactgat gataaaggtg tagcatagat atttactcgg cgatttcagg
      181 atcttatctg aaatcatgcc accaatcggt ccaccaatca ttttcagaca gtattggtta
      241 atgatgccgt aagcccccac cagcgcaacg ggcaatagat agatgttttt caggaatgga
      301 atgaagaatg tcaggccaca gtaaaccgcg taaacgaaga agacgttgaa agcgataagc
      361 cagatggttt tgtctctcag cactgaggtc atactggtgt tttgcgatgc tccgtcttct
      421 tttttcacct caacggacgg tgcctcttct ttgtcattca ggacaaagaa aataataatg
      481 cctacggcaa tcacaataag ggaatagaac cagatgcctg ctttaaaacc taataagcca
      541 ctgccaaacc aggtaaatac tgccaacgca gaaaatgcca ccacggtatc gacaatgcca
      601 cgccctgttt cgaagaagcc aaacaaccga ccttgttgtt cgctattgcc caatcgactt
      661 accgacttga gcaagaccgg ccagttcatc atgtcgcaag taacgccaaa tagcgcccag
      721 acaaagagga tgccccaata ccccggcatt gtcgtcaaat aaacacccag taatcctgtc
      781 gcaatgagtg agaatgacat ggtgtatctg cgcggtagtt tatcggcaaa ataaatagac
      841 agaaaaaagc ccactgtggt gacaaatgag tttaccgaca tagcattacc aatttgacca
      901 ttggtcaaat ggaaatattc ctgcatcggg atataaaacg catctttcag cgacggtaat
      961 ttataaattg taccgccaca cagtgttaat agactaaata acgcccattt ctttttcgtg
     1021 agcataacat cccctaagcg atttttaaaa tcactgtttt aaaggcaata agataccgac
     1081 cattcatatg gaatggtttt attaactgcg tatgagttaa aacttattag tggttcagaa
     1141 gatggtttgc ctcttgtttg atttgttgta gtacgttacg cacatgattt atctgattaa
     1201 ttgcatcttc tttttcttta cgtaaccgcg catccagtaa atgattttct ggtaggggtg
     1261 tttcactcat ctggcgataa gggatccacg gattatcatc ttcgtcttca aagcggaacg
     1321 ccgacgcata gtaatcaacc tcttcttcca ggccatatgc cgtcacctgc ggctcatcat
     1381 cgccaaggca gataaggtgc gttaataacg ctttgaatgg catatcaccc tgacctgaaa
     1441 tacaggcttt atgacccagg ccaccctgtt ctttaacgat caaggcatct ttaatatgga
     1501 cctgggtaat atgcggtgcc atcgttttta aggcgtcgat gggatgctca tttgcattga
     1561 tcatgttagc aaaatcaaac agtaaggaaa gagactccat ctcactttct ttgaccagcg
     1621 acaccagctc atgacttttt aaatcttcat gctgctcaat cgtaaaagtc aggccgctgt
     1681 cctgatacgt ttcccgtaca taggcaatgt cgttagcgat aatcgataat acgtcgcgca
     1741 aattaccttc ataacatgga taaaaacgta cggacgttgc cccagttttc aacgcgatgg
     1801 cgacggcttc gtcaatagat gccttatctg aagcgctggt ttcaatatga atatcaaggt
     1861 tcagacggcg ggctttatcc ccaaaggcag agagttcttt atcgtccata ttaccaagag
     1921 aaaaacgctc gccatccaga acatggattt tgacgccacg tagattattt tcacttgcga
     1981 tatcaagtaa atcagcaggc aacactcttt ccagccgcat atttaaatga aaggcataag
     2041 catgtaaata aagaggaaga gtgtcgacac gttgtagaat ctttcttgcg ttatttattg
     2101 tcaccataaa acacctacag attaatgatg aggtctacgg cctcaaatcg ccaatattca
     2161 atatctatct ggacaatatt gccttgctgg tcggcgcgat gcttttcaat taagatcgct
     2221 ggcatacccg cagaaccgcc aagatacttg ctgatatcgc ccggcattct gacgggtttg
     2281 aaaatcaatt tttttactac cgtctctttt tggtagcact tttcccagac tgcagaaaat
     2341 gagtggtttt caagttgttc aataaaaccc ggagcaactt caggattaat atatgtttcg
     2401 tgatagaaaa ctttatgtcc ttccagcgcc ccccagccgg tgatgcgata gagttcagtt
     2461 gatggcgtca ctgcaatcag tggcgcgagc gtctcgggaa tatcgctggt acggtttttc
     2521 tcggtaaacc cccaagaagg ctctcgccct tgctcaattg cggcccgctg aaagctcgcc
     2581 gacagctccg gactgtaatt aaatcgcggc tgggtcacga accacccctt acgatcctta
     2641 cggaagattt tggattctgc ctcgaggttc aacaacgcct ggcgcagcgt catacgttta
     2701 atgcccagca gttctcccag ctctctttct gaagggagtt ttccacccgg cgtggtgatg
     2761 ccatctttca accaccgatc cagtttctct tttgcatttt ctacggtgga ttgattctcc
     2821 gccattgttt aatccttttg gtttaaacca gatgaagcat cttacgctta cgccaaaaaa
     2881 gaaagcaatt aacggtgtat gactttgcag ataattgtga caaagctctc tattgacgta
     2941 atcacagact tataagggag atactcgcat tggtgtgcgc tcacgacagg taaaaaaaaa
     3001 cctgccatcg ctggcaggtt ttttatgact aaaaaacgaa attaatcgtc tttcggtgcg
     3061 cggttggcgc ggcgacgatc gttttccgtc aggtgacgtt tacgaatacg gatagaggtc
     3121 ggagtcactt ctaccagttc gtcatcatcg atgaactcca gagcttgttc cagagtcatg
     3181 cggataggcg gaaccagaac aacagcttcg tcagtaccgg aagcacgcat gttggtcagt
     3241 ttcttaccgg tcaggcagtt tacagtcagg tcgttagagc ggctatgaat accgataatc
     3301 tgaccttcgt aaacttctgc accgtgaccg aggaacagct taccgcgatc ctgcagaccg
     3361 aacagcgcga acgcgacagc tttaccctga ccgttagaga tcagtacacc gttctgacgc
     3421 tgacccactt cgcccggacg tacgtcgtcg tagtggctga aggtggagta cagcagacca
     3481 gtaccggaag tcatggtcat gaactcagaa cggaagccaa tcagaccacg gcttgggatc
     3541 acgtagtcga gacgtacgcg gcctttaccg tctggattca tgtttttcag gtcgccttta
     3601 cgttcgccca gcgcctgcat cacagaaccc tgatgctgtt cttcaacgtc cagcgtcacg
     3661 ttttcatacg gctcttgttt acgaccgtct atttcacgga agattacttt cggacgggat
     3721 accgccagtt cgaaaccttc acgacgcatg ttttcgataa gaacagagag gtgcagttca
     3781 ccacgaccag aaacgcggaa cgcatcggcg tcttcggttt cttctacgcg cagcgcaacg
     3841 ttgtgtacca gttctttgtt cagacgatcg aggatctgac gagacgttac gaacttacct
     3901 tctttaccgc agaacggcga ggtgttaacg cagaagaaca tagaaacggt cggctcatca
     3961 acggagagtg ccggcagcgc ttcaacgttt tgcgtgtcgc aaacggtgtc agaaatgttc
     4021 agttcgccca ggcccgtgat cgccacgata tcgccagctt ccgccagatc ggtttcgata
     4081 cgttccagac cgaggtggcc cagcacttta ccgactttcg cgttacgggt tttgccttcg
     4141 ctatcgatga tagtaacctg ctggttcggc ttcactttac cacgcttgat gcggccaatg
     4201 ccgataacgc caacatagct gttgtaatcg agctgagaaa tctgcatctg gaacggaccg
     4261 tcaaggtcaa cgtccggcgc aggaacgtgg tcaacaatcg cctgatacag cggggtcatg
     4321 tcttccgcca tatcttcgtg gtccagaccc gcgataccgt tcagcgcaga agcgtaaacg
     4381 atcgggaagt ccagctgctc gtcggtcgcg tcgaggttaa cgaacagatc gaatacctga
     4441 tccacaaccc aatcaggacg cgcgccaggg cggtcaactt tgttgataac aacaatcggc
     4501 ttcaggccgt aagcaaacgc ttttttggtt acgaagcgcg tttgcggcat cgggccgtca
     4561 aatgcgtcaa ccaccagcag cactgagtct accatggaca ttacacgttc aacttcacca
     4621 ccgaagtcgg cgtgccccgg ggtatcaacg atgttgatac ggtaatcatt ccatttgata
     4681 gcggtgtttt tcgcgaggat ggtaatccca cgctctttct ccaaatcgtt ggagtccatc
     4741 acgcgctctt gggtttcggc acgagagtcg aacgtaccgg attgttggag cagcttgtct
     4801 accagggtgg ttttaccatg gtctacgtgc gcgatgatgg cgatattacg caatttttcg
     4861 atcacaactt tgcctcaggc attagaaata gcgcgttatt gtacacggat taatcgcact
     4921 acaaaacagg atcacaaaca tcctccgcaa acaagtattg cagagtccct ttgtgatcgc
     4981 tttcacggag cataaaaagg gttatccaaa ggtcattgca ccaatatggt gcttaatgtt
     5041 tccattgaag cactatattg gtgcaacatt cacatcatgg tgcagccctt ttgcacgatg
     5101 ttaagcatga taacgccttt taggggcaat ttaaaagttg gcacagattt cgctttatct
     5161 tttttacggc gacacggcca aaataattgc agatttcgtt accacgacga caatgaccaa
     5221 tccaggagag ttaaagtatg tccgctgaac acgtactgac gatgctgaac gagcacgaag
     5281 tgaagtttgt tgatttgcgc ttcaccgata ccaaaggtaa agaacaacac gtcactatcc
     5341 ctgctcatca ggtgaatgct gaattcttcg aagaaggcaa aatgtttgac ggctcctcga
     5401 ttggcggctg gaaaggcatt aacgaatccg acatggtgct gatgccagac gcatccactg
     5461 cagtgattga cccgttcttc gccgactcca ccctgattat ccgttgcgac atccttgaac
     5521 ctggcaccct gcaaggctat gaccgtgacc cgcgctctat tgcgaagcgc gccgaagatt
     5581 acctgcgttc cactggcatt gccgacaccg tactgttcgg gccagaacct gaattcttcc
     5641 tgttcgatga catccgtttc ggatcatcta tctccggttc ccacgttgct atcgacgata
     5701 tcgaaggcgc atggaactcc tccacccaat acgaaggtgg taacaaaggt caccgtccgg
     5761 cagtgaaagg cggttacttc ccggttccgc cggtagactc tgctcaggat attcgttctg
     5821 aaatgtgtct ggtgatggaa cagatgggtc tggtggttga agcccatcac cacgaagtag
     5881 cgactgctgg tcagaacgaa gtggctaccc gcttcaatac catgaccaaa aaagctgacg
     5941 aaattcagat ctacaaatat gtcgtgcata acgtagcgca ccgcttcggt aaaaccgcga
     6001 cctttatgcc aaaaccgatg ttcggtgata acggctccgg tatgcactgc cacatgtctc
     6061 tgtctaaaaa cggcgttaac ctgttcgcag gcgacaaata cgcaggtctg tctgagcagg
     6121 cgctgtacta cattggcggc gtaatcaaac acgctaaagc gattaacgcc ctggcaaacc
     6181 cgaccaccaa ctcttataag cgtctggtcc cgggctacga agcaccggta atgctggctt
     6241 actctgcgcg taaccgttct gcgtctatcc gtattccggt ggtttcttct ccgaaagcac
     6301 gtcgtatcga agtacgtttc ccagatccgg cagctaaccc gtacctgtgc tttgctgccc
     6361 tgctgatggc cggtcttgat ggtatcaaga acaagatcca tccgggcgaa gccatggaca
     6421 aaaacctgta tgacctgccg ccagaagaag cgaaagagat cccacaggtt gcaggctctc
     6481 tggaagaagc actgaacgaa ctggatctgg accgcgagtt cctgaaagcc ggtggcgtgt
     6541 tcacggatga agcgatcgat gcgtacatcg ctctgcgtcg cgaagaagat gaccgcgtgc
     6601 gtatgactcc gcatccggta gagtttgagc tgtactacag cgtctaatag ttgaagttgt
     6661 actacccggc gcacaacgcc gggatttagt tgccgtggaa actttcagcc catctctgca
     6721 tgggcttttt tctccgtcaa ttctctgatg cttcgcgctt tttatccgta aaaagctata
     6781 atgcactaaa atggtgcaac ctgttcagga gactgcttta tggcaacagg cacgcagccc
     6841 gatgctgggc agatcctcaa ctcgctgatt aacagtattt tgttaattga tgacaatctg
     6901 gcgatccatt acgccaaccc cgccgcgcaa caactgctcg cccaaagctc ccgcaaattg
     6961 tttggtacgc cgttaccgga actgttgagc tacttctcat taaatatcga gctgatgcaa
     7021 gaaagtctgg aggcggggca aggctttacc gataacgaag tgacgctggt catcgatggt
     7081 cgctcgcata tcctttctgt gacggcccag cgtatgccgg acggcatgat cctgctggag
     7141 atggcaccga tggataacca gcgccgctta agccaggaac aactacaaca tgctcaacaa
     7201 gtagctgccc gtgatttagt gcgcggcctg gcacatgaga ttaaaaatcc gcttggcggt
     7261 ttacgtggcg cggcgcagtt actcagcaaa gcgttacctg acccatcact actcgaatat
     7321 accaaagtga ttatcgaaca ggcggaccgg ctgcgaaatt tggtcgaccg tctgttgggg
     7381 ccgcagctgc ccggtacgcg cgttaccgaa agtattcaca aagtggctga acgcgtggta
     7441 acgctggtgt cgatggaact gccggacaac gtgcggttga ttcgtgatta cgatcccagc
     7501 ctgccggaac tggcgcacga cccggatcaa attgaacagg tcttgctgaa tattgtgcgc
     7561 aatgcgctac aggcgctggg gccggaaggc ggtgaaatca ttctgcgtac ccgcaccgcg
     7621 tttcaactga ccttacacgg cgagcgctac cggctggcgg cgcggattga tgtggaagat
     7681 aacgggccgg gcattccgcc tcatttgcag gatacgctgt tttacccgat ggtcagcggc
     7741 cgcgaaggtg gcaccgggct tggcttatcc atcgcccgta atttgattga tcagcattca
     7801 ggcaaaattg aatttaccag ttggccaggt cataccgagt tctcggttta cctgcctatc
     7861 aggaaataaa ggtgacgttt atgcaacgag ggatagtctg ggtagtcgat gacgatagtt
     7921 ccatccgttg ggtgcttgaa cgtgcgctcg ctggagcggg tttaacctgt acgacatttg
     7981 agaacggcgc ggaagtactg gaggcgctgg cgagcaaaac gccggatgtg ctgctttcag
     8041 atatccgtat gccgggaatg gacgggctgg cgctgctcaa gcagattaaa cagcgccatc
     8101 caatgcttcc ggtcatcatt atgaccgcac attccgatct ggatgctgcc gtcagcgcct
     8161 atcaacaagg ggcgtttgat tatctgccca aaccgtttga tatcgacgaa gcagtggcgc
     8221 tggttgagcg cgctatcagt cattaccagg aacagcagca gccgcgtaat attcagctta
     8281 acggcccaac gaccgatatc atcggcgaag cgcaagccat gcaggacgtg ttccgtatta
     8341 tcggtcggct ttcgcgttct tctattagcg tgctgattaa cggcgaatcc ggcaccggta
     8401 aagaactggt cgctcatgcc ctgcatcgcc acagtccgcg caccaaagcg ccgtttatcg
     8461 cgctgaatat ggcagctatc ccaaaagatt tgatcgaatc agaactgttt ggccacgaga
     8521 aaggcgcgtt tactggcgcg aataccattc gtcaggggcg ttttgaacag gccgatggcg
     8581 gcacgttatt tctcgacgaa attggtgata tgccgctgga tgtgcagacg cgtttgctgc
     8641 gcgtgctggc agacggtcag ttttatcgcg ttggcggcta tgcgccggtg aaagtggatg
     8701 tgcggattat cgctgccact caccagaatc ttgaacagcg ggtgcaggaa ggtaagttcc
     8761 gtgaggatct gttccaccgc ctgaacgtta tccgcgttca tctgccgccg ctgcgcgaac
     8821 gtcgggaaga tattccccgt ctggcgcgcc attttttaca ggttgccgcg cgcgaactgg
     8881 gcgtagaagc gaagttgctg catccggaaa ccgaagctgc tctgacgcgt ctggcgtggc
     8941 caggcaacgt gcgccagctg gaaaacacct gccgctggct aacggtgatg gccgccgggc
     9001 aggaagtgtt gattcaggat ttgcccggcg aactgtttga atcaacggtt gcggagagta
     9061 cttcgcaaat gcaaccggac agttgggcga cacttttagc gcagtgggca gacagagcgc
     9121 tgcgttccgg tcatcaaaat ctgctttccg aagcgcagcc agagctggag cggacgttac
     9181 tgacgaccgc gttgcgacat acgcaggggc ataaacagga agcggcgcgg ctactcggct
     9241 ggggccgcaa cactctgacg cgtaagttaa aagagctggg gatggagtga ttcacggctt
     9301 gtgtgtaaag attgattatt gagcgcaaat tgctggtatt ttgcgcttta ctgttccgat
     9361 aagttcagta tgatcttgcc cggaaaacgg ggagagtcat tatgctggaa tcaataatta
     9421 atctggtatc gagtggcgca gttgacagcc acacaccgca aactgccgtt gctgccgtgc
     9481 tgtgtgccgc gatgattggg ctgtttagct gaagctcgtt gtttatgccg gatgcggaga
     9541 aagtcgtagg cctgataaga cgcgaccagc gtcgcatcag gcatcatgct cagatgcctg
     9601 atgcggcgta aacgccttat ccggcctaca aaagcaataa tttcaacagg ttttgttgcc
     9661 cagcgcatca ggcaatcttg ttctcagcac attgatggcg ctttcgtttt ctactttgta
     9721 aacgaagcgc cattcactct ttaaatcacc cgtgagaact gctgcatccg cgctttctgg
     9781 cgcagatagg tatcaaagca catgcaaatg ttgcggatca gcaagcgacc tttcgccgtc
     9841 acctgtattc ccttctcatc cacatccacc agcccatctt ttgctaacgg ggcgagcagc
     9901 ttgagatctt ccgcaaagta atcagcgaag tgcaaatccc actgtttctc aagaggggcg
     9961 taatccagac ggaagttgca gatgagcgac ttaatcacat cgcggcgaat acagtcatca
    10021 cgcgttagcg caataccacg ccacagcgca ttgccttgtt catacacttg ctgatagtac
    10081 tgcttcaact ctttctggtt ctgcgcgtag cagtcgccaa tcatgctgat ggcggaaacg
    10141 cccatcccca gcagatcggt atcgccctga gtggtgtagc cctggaagtt acgatgcagc
    10201 acgccttcac gctgggccac cgccagctcg tcatcaggac gggcaaagtg atccataccg
    10261 ataaactgat agcccgattg cgtcaggaag gcgatggttt cctgcaggat atcgagtttt
    10321 tgctgcggac tcggcaggtc agcatctttg attttgcgct gagcagcaaa aatggtcggc
    10381 agatgcgcgt agttaaaaac gctcagacga tcggggttca gctccgccac acgtttcagg
    10441 gtaaaggcga aactctccgg cgtctgtttc ggcaggccgt aaatcaggtc gatgttggtg
    10501 gaggtaaaac caatctcacg cgcatggtta agcagtgcaa agatgaactc ttcatcctgc
    10561 tcgcggttaa ccagacgttg cacttctttg ttgaagtcct gcacgcccat gctcaggcga
    10621 ttaaagcctt cggcgcgtaa atgatcgagt acatccagtt cgatttcccg cggatcgact
    10681 tcgatcgaaa tctccgcatc ggtattgaaa tggaagtttt cgcgcagcag cttcatcaga
    10741 cggctgattt gcgctttatt cagatacgtc ggcgttccgc cgccccagtg caattggctg
    10801 acgtgacgcc cggcgaacag cggtgcacga tggacgattt cttgctccag cgcgtccaga
    10861 tactgatcgg ccttgtgctg ctggcgagta acaatcttat tgcaaccgca gaagtaacaa
    10921 agcttatggc agaacgggat atgtacgtag agagataatg gacgctcagg atagcgcgcc
    10981 acggcttgta aaaacgcctg ttcgccgaag tcttctgaaa actccagcgc ggtcgggtac
    11041 gaggtgtatc gtggcccgga atagttatat ttctggatca gggccagatc ccagtcgatt
    11101 tgctgtacag acatgctcac tccttccgat ggcgtctctg gcggctacgg cgtaccggcg
    11161 gatgcccgtt tcgggtcatc agccggttgc gcagccactt ttgtcgccgc gacaaccgct
    11221 gtagtttaac gaataaccac accagataac atataaccgg aagggttatc agcagaacgg
    11281 gaaggcccac ggcggcaatc cgttagttgc cccgcagcag acgcatcatg tcttcttgct
    11341 tctcgtcttc ttcctcttct tcgtcatcgt cataagagag gccgagtttc tgcatcaact
    11401 catcaatacg atccagttta gcatccaccc aggattgctc ttcggcgctc agggtttcgc
    11461 ctgcttccag acgttccagc agcgcatcca gacgctcatc cgtttccagt aattccaact
    11521 ccgcctgcgg tgaaagcata ggtttctcac tcttcggttt gtgctgtttg gtgacttttt
    11581 cagtcactcc caatggaata ggggttttac tgccaatacg tggatctttt ggtgcgttct
    11641 ggcctttgct gcctgacgtg gtgtttccgc ccgctgcgcg gctgcccggc gcatgaccac
    11701 gacgtttttt ctgacgctta cggtcacgag cttcctgatc cagctcctcg cgtgtttttc
    11761 gacgcgcttt tgcatgacct ttgctgcgtg agtttgaaga tgatggtttc ataatggttc
    11821 gtcttttagc cgttttattc agtatagatt tgcggcggaa tctaacagaa agcaagcaaa
    11881 gaaaaaaggc gacagattac tctgtcgcct tttttcctga ctcataaccc ttaacgggtc
    11941 ttacaatcct tgcaggccgc caacacaccc tgtttttcca ttagccggaa caatccgtgt
    12001 tgattccatt tccgtttaat tacgtctccg gacgtttgtc ttcctgacaa tcctctgtct
    12061 tcgcctcctg gcgctcctga ccctcatcct gagtcattgt tcctgttagc gtcctcgctc
    12121 tatgggtgct actttacgcc tttgctttaa acaaacaatc aaccgccatc agtatttgca
    12181 cattttcaga gttttatata aaagcaattc actgatttat atagcttatt tgacatgcat
    12241 cgcagaattt tctctgctat ttcgcataaa gactatcggc aatgtctcac aaagcgtatg
    12301 gcttttactt acaacgctca cgccgaaaaa tccgcctttt attatggttc aggtataatc
    12361 gccacaaagc tttgactgac ggagacgacc gctttgacta atttgaatta tcaacagacg
    12421 cattttgtga tgagtgcgcc tgatattcgc cacctacctt ccgataccgg aattgaagtg
    12481 gcttttgcag gccgttccaa cgcaggtaaa tccagcgcgc tgaacacgct gactaaccag
    12541 aaaagcctgg ctcgtacctc aaaaacccca gggcgcaccc agcttatcaa cctgtttgaa
    12601 gtggctgacg gcaagcgtct ggttgacttg cctgggtacg gttatgcgga agtcccggaa
    12661 gagatgaagc gcaaatggca gcgtgcgctc ggcgaatacc tcgaaaaacg tcagagcctg
    12721 caaggtctgg tggtgctaat ggatattcgc catccgctga aagatttgga tcagcagatg
    12781 attgagtggg cggtagacag caatatcgcc gttctggtgc tgctgaccaa agcggacaaa
    12841 ctggcaagcg gcgcacgtaa agcgctattg aatatggtgc gtgaagctgt actggcgttt
    12901 aacggtgatg tgcaggttga aacgttttct tcgttgaaga aacaaggcgt agacaagctg
    12961 aggcagaaac tggatacctg gtttagcgag atgcagcctg tagaagaaac gcaggacggc
    13021 gaataatttt cttgccttaa tgcttgtgcc ggatgtggcg tatccggccc gtaaattcaa
    13081 tagattacaa ttctaccgtt ggcctgataa acatagcgca tcaggcatta cggatctttt
    13141 ctttcgccca ataaaaaacg ccccagtcat tactgactgg ggcggctaaa atattcagcc
    13201 aaatccgatt acgtgaagta aaaggtctga aagatagaac atcttacctc tgtaccctac
    13261 gcgactaact ttactctttt ttgttcagtt cagaaagcac tttttgtaat tttttttcag
    13321 ttgttgcata ggaatttcta atagccatca caaaacgtga cagcttatgt tgcttactta
    13381 cgaaaaaagg gcatgttcag gcgaatctta gtgcgcctga tcccagtttt cgccactccc
    13441 cacttccacc agcaacggca catccagacg ggtacagttt tccatcagtt gatgaatctg
    13501 cttcgcgacg gcatcaacat catctttatg aacttcaaat accagttcat cgtgtacctg
    13561 catgatcata cgtacacgcg gttgctcagc ctgtaaccac gcatcaacgg caatcatcgc
    13621 ccgtttgata atgtcggcgg cggttccctg cattggcgcg ttaatggctg cacgttcagc
    13681 cgctgcacga cgagcaccat tgctggattt gatatccggc agatacagac ggcgtccgtc
    13741 cagcgtttca acgtagccca gctctttcgc ctgagcacgg gtgcgttcca tatactccag
    13801 cacgccaggg tagcgttcga agtaaaggtc catgtacttc tgcgcttctt tacgtggaat
    13861 gttcaattgc cgcgccagac cgaaagcact catgccataa atcagaccaa agttgatcgc
    13921 tttcgcgcta cggcgttgct cgctggtgac ggtttccagt ggcaaaccaa acacttctgc
    13981 cgccgttgcc cggtggatat cttttccttc cgcgaatgcg gtcagcaagc ctttatcacg
    14041 cgagagatgc gccataatgc gcagttcaat ctgcgagtag tccgctgaga caatcacata
    14101 atcctctggc gcaataaacg cctggcggat acgacgacct tcttcgttac gcaccggaat
    14161 gttttgcagg ttaggatcgg ttgacgataa acgtcccgtt gcagttactg cctggtgata
    14221 agaggtatgc acacgcccgg ttttcgggtt gatcatcagc ggcagcttgt cggtgtaagt
    14281 cgacttcagc ttcgccagac cacgatactc cagaatcact tttggcaacg gatagtccag
    14341 cgccagttct tccagtacct cttccgacgt tgacggcgcg ccacccggcg ttttcttcag
    14401 cggtttaata ccctgttttt caaacagaat ggtttgcaac tgcttggtgg aagaaaggtt
    14461 aaattcttca cctgcaattt catgcgcttt cttttccagc tcagccagac gcaaggtgag
    14521 ctcttcagaa tgattgtgca gcactttcgg atcgatcttc acaccgttac gttcaatgcg
    14581 tgaaagcacc ggcaccagcg gcatttcgat attctcgaag acgttcaacg gccctttgtg
    14641 tttctgcaga tccggccaca ttttcagatg caactgcaag gtgacatctg catcttcggc
    14701 ggcgtaacgt ccggcctctt cgagggcaat ctggttaaag gtcagttgat ttttgccttt
    14761 acccgcaatc tcttcaaaag tgatggtttt gtgcttcaac caacgttccg cgaggctgtc
    14821 catatcgtga cgcccggcaa cgctattgag aatgtaggac tccagcatgg tatcaaacgc
    14881 aatcccacgc agttcaatgc cgtaattcgc cagaataccg cgatcgtatt tcaggttttg
    14941 cccgaccttc agcgcctttt catcttccag cagcggtttt agcaactcga gtgcacgctc
    15001 gtgagagatt tgatcgggcg catcaagata atcatgagca accggaatat atgccgctac
    15061 gcctggctcg atagcaaaag aaagcccgac caggttagca gagatgttat caaggctgtc
    15121 ggtttcggta tcaaatgcaa ataccggcgc tttttccagc ttcgcaatcc acgctttcag
    15181 tgtttcttca tcaaggatag tgacgtagtt gtcataagaa atcaccgttg ccgtcacttc
    15241 tggcgcttcg tctgcaacac tggtttcctg cggcttcgcg gctgtttttg cccctttggc
    15301 ctgtaaccat ttgcccactt cgacatcagc agtccagcgt ttgaactcat actttttgaa
    15361 cagccccaac aactcttccg ctgccggttg ctgcacttcc agttgttcac aggtcagctc
    15421 cagttcaaca tcggttttaa tcgtcgccaa ctggtatgag agataagcaa cttctttgtt
    15481 ttgctcgagc ttcgctgcca ttgttttcgc gccacggaag ctcaacccag caattttttc
    15541 tggctcggca tacagcgtat ccagcccgcc aagaccttgc agcaatgcct gcgcggtttt
    15601 ttcaccgacg cccggtacgc caggaatgtt atcagaggag tcacccatca gcgccaggaa
    15661 gtcgatgatc aattctggcg gcacgccgta cttattcacc acctcttccg gtccgaggat
    15721 ggtattcgtc atggtattga taagcgtaat atttggcgtc accagctgcg ccatatcttt
    15781 atcgccagtg ctaatcagca ccggacgccc ggctttttcg gcttcgcgcg ccagagtacc
    15841 gataacgtcg tccgcttcta cgccagaaac cgccagcagc ggcagtccca tcgctttaac
    15901 catcgcgtgc agaggttcga tttgtgctcg cagatcgtcc ggcattggcg ggcgatgtga
    15961 tttgtaatgt tcaaacagtt catcacgaaa ggtttttccc ttggcgtcaa agaccaccgc
    16021 tgcatgcgtc ggtttatatt gcatgatcag actgcgcagc atgttgagga caccatacat
    16081 cgcaccggtc ggctcgcctg cgctgttagt cagcggggga aacgcgtgat atgcgcgata
    16141 aagataagat gaaccatcta caaggataag tggattttgg gggatctgaa ccataatgtc
    16201 cgtgcctgtt tatcagatta tgggtaaagg atgccacaga caggatgaaa acatgagttt
    16261 ttcaccgctt ttcgtgaaaa tcttttgtag atctgctgga tcgctcgcaa atcgtcatgt
    16321 ggataacttt gtgcataaat tatttacatc gcttttaatt tattatcatg ctttttacag
    16381 catgatgttt gtctttgttt ttcatgcagt taaaaataaa tccatcccta ataattgaga
    16441 taatgggatt ttttctaatg tggatagata tgaattattt ttctccttaa ggatcatccg
    16501 ttatttgggt cattttctgc taaaatctgc gccttgcgct tctttagtgc actgtctatg
    16561 gctaaccttt tgaataaatt cattatgacg agaatactcg ctgcgataac ccttttgctg
    16621 agtatcgtat tgaccatttt ggtcactatt ttttgttccg tcccgatcat cattgccggg
    16681 attgtaaaac tattgctacc tgtgccagtc atttggcgaa aggtttcgcg tttttgtgat
    16741 tttatgatgt attgctggtg tgaaggtctg gcggtattac tgcatctgaa cccacacctg
    16801 caatgggaag ttcacgggct ggaagggtta agtaagaaga actggtatct gcttatttgt
    16861 aaccaccgta gttgggcaga tattgtcgta ctgtgcgtgc tgtttcgtaa gcacattccg
    16921 atgaataaat atttcctcaa gcaacagctg gcctgggtgc catttcttgg cctggcgtgc
    16981 tgggcgctgg atatgccgtt tatgaagcgt tattcccgcg cttatttatt acgtcatccg
    17041 gagcgacgtg gtcaagatgt tgaaaccact cggcgttctt gtgaaaagtt tcgcctgcat
    17101 cccaccacca ttgtcaattt cgttgaaggc tcccgtttca cgcaagaaaa acatcagcaa
    17161 acccactcca cttttcaaaa cttgttgcca ccaaaggcgg caggcattgc gatggcgcta
    17221 aatgtactgg gtaaacaatt cgataaactg cttaatgtta cgctgtgtta tccggacaat
    17281 aaccgtcagc cgttcttcga tatgttaagc ggtaaactga cgcggattgt cgtacatgtg
    17341 gatttacagc ctattgccga tgagttacat ggcgactaca tcaatgacaa aagctttaag
    17401 cgccattttc agcaatggct gaactcattg tggcaggaaa aagaccggct tctgacgtct
    17461 ctcatgtcat cacagcgtca gaataagtaa gttttactcg cctctgaatg agcagaggcg
    17521 agtgagtatt ttaatgaaca aaacgtccgg cacgagacat aaattcttct tcgctcatct
    17581 cctgtccgtt aaaaacaact ttacccggtg tataacgcag ctgcagcgag gcggtattgt
    17641 tgtcaatcgt gattaactga aacatctgac ctaatgtcat catcccgctg atttgtttat
    17701 cagcctgctt ttgcgctttt tccgcatcca ttccttcaga taaattaagc tgttttgcgg
    17761 tttctgtgac gacattaagc ggtaacttca catcaaaatt gagcgatttg atatctttgt
    17821 ttgtggatga tgaagatttg gctgggtcgg caatactgat atccagattg gcatttagtt
    17881 cgccgagtgc gttcttccat cttaccggtt gtttaatggt cggctcactt ttttgtaata
    17941 acggtaaata ctctttgaac aatgcagcat tcacttcttg cagagcaact tcatcgtttg
    18001 ctaactcagg gtgtgcagca agctgcttct gcatcgcaat gttatactgg atgataaatt
    18061 ggcgcactgc tgagggatca atagattcag caatcaatga ccactcacca ctaccgagtt
    18121 gctgattccc ttttgttaac ttatcaagtt cgtaggtcag attgacattg atataatctt
    18181 tgtctttaac gcgatccagc ctggtttttg caacgaaagc atcgatttgg gcaacgtctt
    18241 ccccgtgatt gataatgtta atctgattaa atttcgactc gctttcacca accggaaatt
    18301 tcttttcttc taatcgcgcc agagaatcaa tggtaaaact ttttgccgtt actttgttca
    18361 ttggtgataa ttgcagaaca atatcttcga ctttgccttc aatatccagg ttagacaatg
    18421 aactaccatc accattaaaa gtaaactggc cctcaccaaa agaaaatttt ccatactctg
    18481 caggggcaac atctaatgtc gtagagaatg agccaccaaa cgcgaatcgc aatgtcgctt
    18541 ccacgggcga tttatttttc gcggcgataa atagcggttg tgtcagttca ttattcacta
    18601 agttcacttt tgccgctgcc agtgctggga tgacattacc atgcattaac attgtgatgg
    18661 gcaacggacc gtgctcaaca tccacatcaa atacaacttt ctggcctggc ttgatattga
    18721 gatcgggtgc accgttatcg aatgtcattt gcatttgaaa acgggaatta aaaaagcctt
    18781 tgtcaaagtt tttataactt aacgtcatat cgtaggcatg ttcacctttc tttttcgcat
    18841 cgttaaaatc tttaataaac ttttcgatac caggctgaat ttgcgtacct gtgtaccatg
    18901 tgccaccacc ccagattacg gctaacgcaa caataacacc tgtagctgac ttacgtatca
    18961 tataaatatt ccctgataaa acagcaaaac tttgaatatc cacttatgct gaaataatgt
    19021 ccagcggcag gatgcattat cagaatgtat ttatatttat taaataataa aaaaagcccg
    19081 tgaatattca cgggctttat gtaatttaca ttgaattatt tttcctcgga cagatatttc
    19141 accgtatcag catactgctg aacaaaaaca tccatattgc tggtatccat accctgcgga
    19201 ttcagctgat atttaccgtt aacaaacatc gccggaacac cacgcaattg cacatcagct
    19261 gcggcttttt cctgctgagc gaccagagat ttcaccacga agctgttcca cgccgcgtcg
    19321 tactcttcac ctttaatacc tgcattgata aatacatcgc ggatatcaga tgctgaacga
    19381 atggtctggg ttttctgtac gccttcaaac agcggaacag tcactttgtc ttccacgccc
    19441 agcgccatcg ccacagccca tgcctgagtc agatctttgc ccaggtcacc acccatgaag
    19501 ttgacgtggt atttagtcat cttcacgcct tccggcagtt ttttcttcac attatcagaa
    19561 atatgcagaa cttcttcaaa ctgatagcag tgcgggcaga agaaagagaa aaactccagc
    19621 acttgcggcg cgccagctac cggtttttcc agggtagtgt actgtttacc atcttcatac
    19681 tgcgccgccg atgcgctaaa cgctaaaact aaaccagcca gcgccagcca aatctttttc
    19741 atgatctact ctctccgatt aatacatagg tgttaattgc aaagggggtt cttgtagaac
    19801 ttttgcctgt tctataaaag tcgccgtctg tcgtagccag taatcttccc cggttaacca
    19861 cgggaaattt ttcgggaacg cgggatcagc ccaatgccgc attagccagg caagataata
    19921 aaccaaacgc atggcgcgta aaggttcaat cagtccgatt tcagcggtgt cgaactcgct
    19981 aaattcttca taagcttcaa taatagtttc cagttgcatc cgctgctggg ctttatcgcc
    20041 attgagcaac atccacaaat cctgaatagc tggaccatta cgtgcatcat ccagatcaac
    20101 aaacattgga ccatcgcgcc agagaatatt cccggcgtgg cagtctccat gtagccgcag
    20161 aacggtgaaa tcttcccgcc agtgtgctgt aacggcggcg atcagctcat ctgtcgcttt
    20221 cagaaatgcc gctttcaacc cggaaggtat cagtgtagcg tcctcaaaca gcttgcgagg
    20281 ctcaatgaga tattcattca gaccgatggt cgggcgatga ataaaaagct gtttgcgccc
    20341 cgtctggtgc atacgcccta aataacgccc aaccgcttcc atctgatcga tattatcagc
    20401 ttcgaactgg cgaccaccga cgcttggaaa aacagcgaaa taaaatccct gatgattcaa
    20461 taaagtctga ccgttaaagg ccacaggtgc tgcgaccgga acttcatcat ttaccagctg
    20521 caacgcaaat tgatgttctt cgaggatttg atcggctgtc caacgttcag ggcgataaaa
    20581 tttgacgaca aaacgtcgac gctcttcgtc ctgaaattga tagacacggt tttcatagct
    20641 gttaagcggg gtaagaccgg aatccacccg gatcccttgc ttaaacagag cgtccatgat
    20701 ggtatccggg tgtagtgtct ggaaagtaaa agcgctgttg ttcatcccat catccggaaa
    20761 acattacgaa tgatgaagga tatcatttca cgatgatttt ggtggcgccg cttacaagct
    20821 tttactcttt aattacgccg cgagcgcgta acagcgccgt tttgaagtct tcttcataat
    20881 ctttctgcaa acccgggatc accgcatctt tcgcggaatc acgcattttc aggtgataga
    20941 tcagaatgtc atccgtcaaa tccgccagtt cgccgtcaaa acctgactct ttcgccagtt
    21001 tctgcaaaaa ttgcaacaag ttaaagtctg gctctttttg ccaggctggc tggaggagtt
    21061 caataacttc attcagacgt ttacatttca tagtaatgct ccttactctt gagacagaca
    21121 cgttagcagg gtcaatccca caataaaaga ggcgatatcg gtgaatctaa tgacgacgat
    21181 aacaggcgtt gtgctggcag gcggtaaagc cagacgaatg ggcggcgtag ataaaggatt
    21241 gctggactta aatggcaaac cgctgtggca acatgtcgct gacgcgctta agacgcagct
    21301 ctctcacgtc gtggttaatg ctaatcgtca tcaggaaatc tatcaggcag gcggtctgaa
    21361 agtgattgaa gattcactgg cggattaccc aggccctctg gcaggaatgc tttcagtaat
    21421 gcagcaggaa gctggtgagt ggtttttgtt ttgtccgtgc gatacgcctt acattcccca
    21481 tgatttagcc gcccggctta atcatcagcg caaagatgcg cctgtcgtgt gggtccatga
    21541 cggtgaacgc gatcacccga ctattgctct ggtaaaccgc gctattgagc ctttattact
    21601 ggaatatctg caagcaggag aacgccgggt aatggcattt atgcgtctgg ctggcggtca
    21661 tgcggttgat ttcagcgatc ataaagatgc atttgttaac gtgaatacgc cagaggagct
    21721 tgcccgatgg caggaaaaac gatgataccg ttactcgcct ttgccgcgtg gagtggtacc
    21781 ggaaaaacta cgcttttgaa aaaactgatc ccggcattat gcgccagagg gatccgtccg
    21841 gggctgatta agcatacgca ccatgatatg gatgttgata agccaggcaa agatagctat
    21901 gagctgcgca aggctggcgc ggtacaaacc atcgttgcca gccagcaacg atgggccttg
    21961 atgacggaaa caccagacga agaagagctg gatctacatt ttctcgcaag tcgaatggat
    22021 acctcaaagc tggatttgat tctggtcgaa gggtttaagc atgaagagat cgcaaagatt
    22081 gtgctgtttc gcgatggagc cggacatcga cctgaagaat tagtgataga caggcatgtt
    22141 attgctgtag ccagtgatgt gccgcttaat cttgatgtcg cattactgga tattaatgat
    22201 gttgaggggc tggctgattt tgtggtggag tggatgcaaa agcagaacgg ataagcaaga
    22261 gtaggctgga tcagacgcag ccagcgtcgc acccggcatt tttttctgcg ccaa
//
LOCUS       DAEBXN010000065        21778 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.065, whole genome shotgun sequence.
ACCESSION   DAEBXN010000065 DAEBXN010000000
VERSION     DAEBXN010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21778
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.065"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(<1..166)
                     /locus_tag="KJL99_002542"
     CDS             complement(<1..166)
                     /locus_tag="KJL99_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBD4437230.1"
                     /translation="MHSSDQKRVAVLMATYNGECWIEEQLKSIIEQKDVDISIFISDD
                     LSTDNTLNICE"
     gene            complement(159..1049)
                     /locus_tag="KJL99_002543"
     CDS             complement(159..1049)
                     /locus_tag="KJL99_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="HBD4437231.1"
                     /translation="MNSNIYAVIVTYNPELKNLNALITELKEQNCYVVVVDNRTNFTL
                     KDKLADIEKVHLICLGRNEGIAKAQNIGIRYSLEKGAEKIIFFDQDSRIRNEFIKKLS
                     CYMDNENAKIAGPVFIDRDKSHYYPICNIKKNGLREKIHVTEGQTPFKSSVTISSGTM
                     VSKEVFEIVGMMDEELFIDYVDTEWCLRCLNYGILVHIIPDIEMVHAIGDKSVKICGI
                     NIPIHSPVRRYYRVRNAFLLLRKNHVPLLLSIREVVFSLIHTTLIIATQKNKIEYMKK
                     HILATLDGIRGITGGGRYNA"
     gene            complement(1079..2335)
                     /gene="wzx"
                     /locus_tag="KJL99_002544"
     CDS             complement(1079..2335)
                     /gene="wzx"
                     /locus_tag="KJL99_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen flippase"
                     /protein_id="HBD4437232.1"
                     /translation="MSIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGVYTIAI
                     ALVGYAGIFDVGLTRSVIREIAIHRDNHHERTKVISTSTSFLVLFSCFGAFLLLIFSD
                     GIVNYLKISGVEHSDIQLAFKLLAICIPLFILNQLWSAILEGDEKFGIVNIQKSISSS
                     CIAGIPAIFVFYSATLSAAVAGLIFARVISILVSAYYVRNDIKISGVHFCYKTFKRLF
                     FFGGWMTVSNIISPVMVYFDRFIVSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVF
                     PRLSNIKDFKEFKRNVNKSLLLMFLICLPVIIIGLLYSGLVLKIWFGENYQINSFNIL
                     NVLLIGFFFNALAMIPFSAIQALGKSKITALIHCAELVPYLALLYFMVEKYGLLGAAI
                     SWSIRVILDALLLQWLYTRMCSVYEN"
     gene            complement(2351..2896)
                     /gene="rfbC"
                     /locus_tag="KJL99_002545"
     CDS             complement(2351..2896)
                     /gene="rfbC"
                     /locus_tag="KJL99_002545"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HBD4437233.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWPVQ
                     NPLLSDKDINGQKFVDADYFI"
     gene            complement(2901..3779)
                     /gene="rfbA"
                     /locus_tag="KJL99_002546"
     CDS             complement(2901..3779)
                     /gene="rfbA"
                     /locus_tag="KJL99_002546"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HBD4437234.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPQEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMDQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAHRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            complement(3838..4737)
                     /gene="rfbD"
                     /locus_tag="KJL99_002547"
     CDS             complement(3838..4737)
                     /gene="rfbD"
                     /locus_tag="KJL99_002547"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HBD4437235.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPNFAQLLNATCVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            complement(4737..5822)
                     /gene="rfbB"
                     /locus_tag="KJL99_002548"
     CDS             complement(4737..5822)
                     /gene="rfbB"
                     /locus_tag="KJL99_002548"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HBD4437236.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGTYQSWIEQNYEGRQ"
     gene            complement(6195..7088)
                     /gene="galF"
                     /locus_tag="KJL99_002549"
     CDS             complement(6195..7088)
                     /gene="galF"
                     /locus_tag="KJL99_002549"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HBD4437237.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVIIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            complement(7263..8657)
                     /gene="wcaM"
                     /locus_tag="KJL99_002550"
     CDS             complement(7263..8657)
                     /gene="wcaM"
                     /locus_tag="KJL99_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HBD4437238.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGGMHNITLDVRGSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIN
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            complement(8668..9888)
                     /gene="wcaL"
                     /locus_tag="KJL99_002551"
     CDS             complement(8668..9888)
                     /gene="wcaL"
                     /locus_tag="KJL99_002551"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HBD4437239.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGLWERRLRTIIEQYQ
                     LEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLLPENDARALAQRLAAFSQLDTDELAPVVKRACEKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(9885..11165)
                     /gene="wcaK"
                     /locus_tag="KJL99_002552"
     CDS             complement(9885..11165)
                     /gene="wcaK"
                     /locus_tag="KJL99_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HBD4437240.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRIKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRRNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPVLNARFNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            complement(11345..12823)
                     /gene="wzxC"
                     /locus_tag="KJL99_002553"
     CDS             complement(11345..12823)
                     /gene="wzxC"
                     /locus_tag="KJL99_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HBD4437241.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDVI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSIVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(12825..14219)
                     /gene="wcaJ"
                     /locus_tag="KJL99_002554"
     CDS             complement(12825..14219)
                     /gene="wcaJ"
                     /locus_tag="KJL99_002554"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HBD4437242.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(14274..15644)
                     /gene="cpsG"
                     /locus_tag="KJL99_002555"
                     /gene_synonym="manB"
     CDS             complement(14274..15644)
                     /gene="cpsG"
                     /locus_tag="KJL99_002555"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HBD4437243.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARTISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRSDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(15837..17273)
                     /gene="cpsB"
                     /locus_tag="KJL99_002556"
     CDS             complement(15837..17273)
                     /gene="cpsB"
                     /locus_tag="KJL99_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HBD4437244.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSVGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMEPTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(17276..18499)
                     /gene="wcaI"
                     /locus_tag="KJL99_002557"
     CDS             complement(17276..18499)
                     /gene="wcaI"
                     /locus_tag="KJL99_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HBD4437245.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADLLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(18496..18975)
                     /gene="gmm"
                     /locus_tag="KJL99_002558"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(18496..18975)
                     /gene="gmm"
                     /locus_tag="KJL99_002558"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HBD4437246.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(18975..19943)
                     /gene="fcl"
                     /locus_tag="KJL99_002559"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(18975..19943)
                     /gene="fcl"
                     /locus_tag="KJL99_002559"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HBD4437247.1"
                     /translation="MSKQRIFIAGHCGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     MFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRT
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRGE"
     gene            complement(19946..21067)
                     /gene="gmd"
                     /locus_tag="KJL99_002560"
     CDS             complement(19946..21067)
                     /gene="gmd"
                     /locus_tag="KJL99_002560"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HBD4437248.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(21093..21641)
                     /gene="wcaF"
                     /locus_tag="KJL99_002561"
     CDS             complement(21093..21641)
                     /gene="wcaF"
                     /locus_tag="KJL99_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HBD4437249.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(21657..>21778)
                     /locus_tag="KJL99_002562"
     CDS             complement(21657..>21778)
                     /locus_tag="KJL99_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HBD4437250.1"
                     /translation="ADAKKVQRQILHVPGFWAELSWHLRQRTTGKTKALYNKV"
BASE COUNT         5433 a         5741 c         5019 g         5585 t
ORIGIN      
        1 cttcacaaat atttaatgta ttatcagttg ataaatcatc tgatataaaa attgagatgt
       61 ctacatcttt ttgttcaata atacttttta attgttcctc aatccagcat tcgccattat
      121 acgtagccat aagtacagct actctttttt gatcagaact atgcattata acgaccgccc
      181 ccagtaattc ctcttattcc atccagcgta gccaaaatat gttttttcat atattcaatt
      241 ttattttttt gagttgcgat aattaaagtc gtatgaatta aagaaaaaac aacttcccta
      301 atagagagta aaagaggcac atgatttttt ctaagcaaaa gaaatgcatt tcttactcga
      361 taataacgac gtactggcga gtgaattggt atgttaatcc cacagatttt tactgactta
      421 tccccaatag catgaaccat ttctatatca ggaatgatat gaactaatat gccatagttt
      481 aagcatctaa gacaccattc tgtatcgaca taatcaataa aaagttcctc atccatcatt
      541 ccaacaatct caaaaacttc ttttgaaacc atggttccgg atgagattgt tactgaactt
      601 ttaaacggtg tctgtccttc agtgacatga attttctctc gaagaccatt ttttttgata
      661 ttacaaatag gataataatg tgacttatct ctatcaataa aaactgggcc agctattttt
      721 gcattttcat tatccatata gcaggaaagt tttttgataa actcattgcg aatacggcta
      781 tcttgatcaa aaaaaattat cttctctgcc cctttttcaa gagaatatcg aatcccaata
      841 ttttgtgctt ttgctatacc ttcattccta ccaagacaaa ttaaatgaac tttttcaatg
      901 tctgctaatt tatctttcaa ggtgaaatta gtcctattat cgactactac aacgtaacaa
      961 ttttgctcct ttagttcagt gatcagtgca ttcagatttt taagttcggg attatatgtc
     1021 acaatgacag cgtaaatatt actattcatt atccaagtga ctcagtaatt ggttaatttt
     1081 agttttcata tacagaacac attctagtat aaagccattg taataacaga gcatctaaaa
     1141 ttacacgtat gctccaggat attgcggcac ccagtaaccc atatttttcg accataaaat
     1201 acaaaagggc taaataagga accaattcag cacaatgaat caaagcagta attttagatt
     1261 ttcctaatgc ctggattgca gagaatggta tcattgccag cgcattgaag aaaaaaccga
     1321 tcaataacac atttaatata ttaaaggaat taatttgata attctcacca aaccatattt
     1381 tcaatacaag ccctgaatat aacaagccta tgattatcac cggcaaacag attagaaaca
     1441 ttaaaagcaa tgatttattt acattacgtt taaattcttt aaagtctttg atgttactta
     1501 accttggaaa cactgccctc ccgattgctg caggtattat tcctaatttt agaataacct
     1561 ccgctggtgc agaataaaat gcaactttat ctgcccccat aatatttgac actataaatc
     1621 gatcaaaata caccatgacc ggacttatga tattacttac tgtcatccaa ccgccaaaga
     1681 aaaagagtcg tttaaaggtt ttataacaaa aatgaacccc cgaaatttta atatcattcc
     1741 tgacataata ggcagagact aaaatcgaaa taactcttgc aaatattaaa ccagcaaccg
     1801 ctgccgacaa tgtagcacta taaaaaacaa atatggccgg aattcccgcg atgcaagagc
     1861 ttgatataga tttttgaata tttacaatgc caaatttttc atccccctca agaatggctg
     1921 accataattg atttagaata aataatggaa tgcaaatagc caacagttta aatgctagtt
     1981 gtatatcact atgttcaaca ccagaaattt ttagataatt aacaattcca tcagagaaaa
     2041 tcaataataa aaaagcacca aagcatgaaa atagcaccaa aaaagatgta cttgttgaaa
     2101 ttaccttggt tctttcatga tgattatcac gatgaattgc aatttctcta ataacagagc
     2161 gagtcaggcc tacatcaaaa attccagcat acccaacaag tgcaattgca attgtataaa
     2221 caccgaaacc ttcaggcccc aaacctcgag cgagaaatcc tagcgagggg atagcaatta
     2281 aagttggtat tgcatagcca aaaaggttcc agacactatt ttttattatg ctcattatta
     2341 catttcttta tcatataaaa taatcagcat ctacaaattt ttgaccatta atatctttat
     2401 cagaaagcaa tggattttga accggccatt ctatatttat acttttgtcg ctccataaaa
     2461 tcccacgctc ttgcattttt gaatagaaat tatttgtttt gtagacaaat tctgcttcat
     2521 cactaagaac gcaaaaccca tgagcgaatc cttccggtat ccataattga cgcttattct
     2581 ccgccgacaa atttacacca acccacttgc caaatgttgg tgatgatttc cgaatatcaa
     2641 cagctacgtc aaaaacttca ccgacaacac agcgtaccag ttttccttga gcataaggtt
     2701 ccagctgata gtgcagtccg cgtaacacac ccttagttga tttagaatgg ttatcctgaa
     2761 caaactcaac ctttcgtcct acagcctctt cgaaaacttt ctgattgaag ctttccataa
     2821 aaaagccccg ctcatcacca aatacttttg gttcaaaaat tagcacatca ggaatttcag
     2881 ttttaattac gttcatttta ttaataacct ttaatcattt tcagcagata ctgaccataa
     2941 gcatttttct tcagcggctc ggctaatacc ttcacttgct cagcatcaat aaagccttta
     3001 cggtgagcaa tttcttccgg gcaggaaact ttcagcccct gacgctcttc aattgttgca
     3061 atgaagttgc ttgcttcaat aagactttga tgtgtccccg tgtccagcca tgcataacca
     3121 cgccccatca tagcgacaga caaacgtcct tggtccatat aaatacggtt aatatcggta
     3181 atttccagtt cgccacgtgc agatggttta aggtttttcg ccatttctac aacgtcattg
     3241 tcatagaaat aaagcccagt aaccgcatag ttactttttg gttcctgcgg tttttcttcc
     3301 agactaattg ccgtaccgtt atcatcaaat tccaccacac cgtagcgctc aggatcattt
     3361 acgtgatagg caaaaaccgt tgcgccactc tctctgttaa cagcggtatc cattaatttc
     3421 ggcaggtcgt gaccatagaa gatattatca ccaagtacca gcgcacaatc atcaccacct
     3481 ataaattctt caccaatgat aaatgcttgc gccagaccat ccggactcgg ttgtacttta
     3541 tattgcagat taagccccca ctggcttcca tcaccaagca attgttgaaa acgtggtgta
     3601 tcctgcggcg tactgataat cagaatatct cgaatacccg ccaacataag cgttgaaagc
     3661 ggataataaa tcatcggttt atcataaatc ggtagcagtt gtttgctcac agccattgtc
     3721 acaggataaa gacgagtgcc ggaaccacca gccagaataa tacctttacg cgttttcatt
     3781 tcaccattcc ttttaattca tctcgctcta gcatcatgag cgagatgcaa taatttgtta
     3841 aattgccgtg gtcgtaaata attcgttgag catacgtttc acgcccacct gccagtcagg
     3901 caagacaagc gcaaagttct gctgaaattt ttcggtattg aggcgagaat tatggggacg
     3961 acgtgctggt gtgggatagg ccgttgtcgg cacggcatta agtttgttaa gagcaaggtt
     4021 gatccccgct ctgcgcgctt cttcaaatac cagagcggca taatcgtgcc aggttgttgt
     4081 accaccagca accagatggt acaaaccagc aacttctggt ttatttgcgg ccacgcgaat
     4141 ggcatgagcg gtgcaatcag ccagtaattc agcccccgtt ggtgcgccga attgatcgtt
     4201 tattacagcc aactcttcgc gttcttttgc caggcgcaac atcgttttgg caaagttatt
     4261 tcctttacca gcgtataccc agctggtacg gaaaataaga tgctttgcgc aatgttcttg
     4321 taacgctttt tccccagcta gcttggtttc accgtagaca tttagcggtg ctgttgcatc
     4381 cgtttccagc catggcgtgt caccgttgcc tgggaaaaca taatcagtgg agtagtgtat
     4441 aacccaggct ccgacttcat tagctgcttt tgcaatcgct tcgacgcatg tcgcgttaag
     4501 taattgcgcg aaattcggtt ctgattccgc tttgtctact gcagtgtgag cagcagcatt
     4561 aacaataaca tcggggcgaa tttttttgac ggtttcagcc acaccttcgg ggttgctgaa
     4621 atctccacaa taatcagtgg agtgaacatc gagcgcaatc aagttaccca gaggagccag
     4681 agcacgctgc agttcccaac ccacctgccc tgttttgccg aaaagcagga tattcattac
     4741 tgacggccct catagttttg ttcaatccac gactgataag taccgctttt cacattctca
     4801 acccaatttg tattagccag ataccattcc accgttttac gaatcccgct ctcaaacgtt
     4861 tcttgcggtt tccagcccaa ttcgcggcta attttatcgg catcaatggc gtaacggcgg
     4921 tcatgccccg ggcgatcggc aacataagta atttgctcac gataagattt ctctttcggt
     4981 acaatctcat ccaacaaatc acaaatagtg agcactacgt cgatgttttt cttttcgtta
     5041 tgtccaccaa tgttataagt ttcacccgct ttaccttcgg ttacgacggt atataacgca
     5101 cgagcatgat cctctacata caaccagtcg cgaatttgat cccctttacc ataaataggt
     5161 aatgccttac cttccagagc attcagaata accaatggaa taagtttttc cgggaagtga
     5221 taaggaccat aattgttcga gcaattagtc acaatggttg gtaaaccata agtccgcttc
     5281 caggcacgta ccaaatgatc gctggaagct ttagacgcag aatacgggct acttggcgcg
     5341 taagctgtcg tttccgtaaa tagcggtaac gcttcgttat tatttgcttc atccggatgg
     5401 ggtaagtcac cataaacttc atcagtagaa atatgatgaa agcggaaact ttttttcttt
     5461 tcatcattca gagcagacca gtaattgcgc gccgcttcta aaagaacata agtccccaca
     5521 atattggttt caataaatgc cgcagggccc gttattgaac ggtcaacgtg gctctctgcc
     5581 gccaggtgca tcaccgcgtc tggctggtgc tgtgcgaaaa tacgcgacat ggctacagca
     5641 tcgcagatat ctgcatgctc aaaagcataa cgttcagaat cagaaacatc agcgagtgat
     5701 tccaggttcc cggcgtatgt taatttatcg acattaacaa cactatcttg cgtattattt
     5761 attatgtgac gaacaacagc agaaccaata aaccctgcgc caccagtaac aagtatcttc
     5821 acttttttat tccatatagc tatagagcat gctgtgaaat agactgctcc agatttgatt
     5881 aatagaggta ttaatacacg ctaccgcccc tggcttaaca gctaccagtg cactgcatgc
     5941 atctcaacga tgtggcgagc gttagccact cgcgccaaat ccgaaaaatt caaacgttaa
     6001 ttgtcttacc aaacagccgt agaaacaagg aaaatcctgg aaaaatttga ataatatgag
     6061 ctatgtaact tcttgttttt ataggcttta taaaaacggc aacggcagga aaattcaatg
     6121 agcaaaactt atgccacgaa tcttcactgc cgttataatt ttcttatcaa ccgttacatc
     6181 cggtcagatt ttcattattc gcttaacagc ttctcaatac ctttacggaa cttcgcccct
     6241 tctttcaggt tgcgtaggcc atacttcaca aacgcctgca tatagcccat ttttttgccg
     6301 cagtcgtaac tgtcgccggt catcagcatt gcatcaacgg attgtttttt cgccagctcg
     6361 gcaatagcat cagtcagctg aatacgtccc catgcaccag gctgagtacg ttccagttcc
     6421 ggccaaatat cggcagaaag cacatagcga cctacggcca tgatgtctga gtccagcgtc
     6481 tgcggctgat ccggtttttc gataaattca acaatgcggc tgactttacc ctcacggtcc
     6541 agcggctctt tagtctggat gacggagtat tcagagaggt cacccggcat acgttttgcc
     6601 agcacctggc tgcggcccgt ttcgttgaaa cgtgcaatca tggcagcaag gttgtaacgt
     6661 agcgggtcgg cgctggcatc gtcgatcaca acgtctggca gtaccacgac aaatgggttg
     6721 tcaccaatgg caggtcgcgc acacaaaatg gagtggccta aacctaaagg ttcgccctga
     6781 cgcacgttca taatgatcac gcccggcgga cagatggact gtacttccgc cagcagttga
     6841 cgcttcacgc gctgctcaag gagtgattct aactcataag aggtgtcgaa gtggttttcg
     6901 accgcgttct tggacgcgtg agttaccagg aggatttctt tgatccctgc agccacaatc
     6961 tcgtcaacaa tgtactgaat cattggcttg tcgacgattg gtagcatctc tttgggtatc
     7021 gccttagtgg caggcaacat atgcatcccg agacccgcta caggaataac tgcttttaaa
     7081 ttcgtcatta tttcatccac ctgtaaaatg gttgctgaat tatagcttgt tcgatttttt
     7141 tcgccagcat caattaccct gaattgatta ctgaattact tgtgatgtta cgccgcttcg
     7201 ttgtggattg cagtagcatt gttcctaagt atggctccat ttttccagga atggtcgcaa
     7261 atctactccc tccgttccgg caatctaaag ttaatctttt ccacattaac aatatggtga
     7321 ttaatcctgt cgatatcgac ggagctttgc cctctttcat tcaccgcatg aacatttgca
     7381 agagacagca gtgtttcttt tttcgccata aagacgccgc gaacgtcttt gcgcatgtcg
     7441 aagttcatgc tcaatgctgg tccaactgag gattcctgca tcacattgat atttcgcata
     7501 aaaagatgtt gcggcttgtt gtgtaactcc agcgaggcac gcttcatctc aatgttagtt
     7561 agcgacacaa aggagacggc attcccggcg gagatttgga tgccgcgcaa tttataagca
     7621 agatgggtat tatccagttg aatattattc actcggaaat tttgcggtat cgagagatat
     7681 ttgcctttaa ttaccccata gccgattaac atcccggcgc tattaatcat ttcaatatta
     7741 tcaatcacga aattgtcaca accgtaaata gcgactgtcg cgttatcaat gcccgctttc
     7801 ttactgaaat ccggcgtgat attgcgggca ttgatattac gaataacaaa atgtttacca
     7861 ttttcaacat ggatcaactg ccgacaatcc gatcccgtga tattcgccac gacaaagttt
     7921 ttcactgcct ggtcttccgg gtagttgtta tcataagtgc ttcccgcaag gcctatgccg
     7981 atgccccagt tgattttgcc gttggtacag ttgatacgct cgatgacatg gtcagatatc
     8041 aaaatatcac tgtcgttaat tgccacgttc cattcaatgg cgtcgccctg taagtcgctg
     8101 aacttacagt tggtaatgtt ggcaccgata atctggttat gaaatccctg gcgtaagatg
     8161 gcgtaattag cgtggctgac agtgaggtta tcgatggtca ggttgcgcat gacccgtttg
     8221 tttttgccgc caatataaat ctgcattacc gggccaaagc cgctcatcgc cagccctttg
     8281 atggtgcagt cagaaccacg cacatccagg gtaatgttat gcataccgcc gccctctccc
     8341 cctgtcacct ggctgccgtc ctgtaagaca aatcgccctc tgccgttgcc gcgcaggctt
     8401 ccaaggatgt gtaacgtttt gccgggaggg atgaagatgc cggtgttgat attgtcacaa
     8461 acgaatccgg caggcacgac gaccgtttgc ccttcgctga aggcttgttt aaatgaggcg
     8521 atccagtcat gtgggttgta gtcgttaatg ttaacgcttt gtcgggcggg aagtgcgcga
     8581 gcgaaagggg tatggaggaa ggcaagcgcc gagcttgccg tcaggaaggt gcgtcgggag
     8641 agtgttttaa atggcatacg ttctcctcta taaagcctgc agcaagctgg cgagttctcg
     8701 attaatcacc tgctggttaa aatcgtgttc gactttttcg cacgcacgtt tgacgaccgg
     8761 agccagttcg tcggtatcca gttggctaaa cgccgccaga cgttgtgcca gtgcgcgagc
     8821 atcgttctca ggcagcagcc agccggattt atcggcctcc accagttcag gaattccgct
     8881 atgcagagta gaaaccaccg gaataccgac cgccatcgct tccattagcg ccaccggaat
     8941 gccttccata tcgccatccg cgcccgttac cgatggcaac aggaagacat ccgcgtcgtc
     9001 gagcatctcc ttcacttcat ggctcggttt aaagcccggc atctctacca catcttccag
     9061 ttgatattgt tcgatgatgg tgcgcaggcg tcgttcccac aggccaatgc cgaggatgcg
     9121 atagcgaaat gccacgccct gctctttcaa ctgacggcag gcttcaatcg ccacatgcag
     9181 gccttttttc tcggttaagc gtgcgacaga tatgatttcc agcggcgttg cgggcgcttt
     9241 caccggacgc gggctaaagc gcgtcatgtc cacgcccata cgcgatacgg cgattttttc
     9301 tctggggcag cccatttttt gcagccttcc ggcccacaga tcgcttatcg gtaacatcag
     9361 gtcgccacgg cgaaacagtt gttgatattc gggagtgtag tggttgagca cttcccgact
     9421 ggagatatca ataccgtgga agatggtggc aattttgccg cgaatgatac ccagttcgcg
     9481 tagttttgct gcggttaccc ccgccgggcc aaaatgagcg atgaacacat cggcgcgaaa
     9541 cggtgttgcg acctgaccac aaatggcaga caaaatcagg ttccgcgact cggcaccata
     9601 gcgtttgagg ttgagcgcat gccaggtatt tttacgatga atgccacgta aggtctggct
     9661 ggcgcggtgg cgcagtttcg ctactttgcc cgcaggttcg tcctgtaacc agcgggttct
     9721 ggcgacaagg ttgtatttcg tccatgccgc gtgggtgttc tctgtatcgc ctttttgcaa
     9781 agcgacaatc tccacttcaa agcccatatc aataaacgcg gtaatttgat tgaggacgaa
     9841 ggtttccgac gacagcggga atttcaataa gaagaagccg accttcattt cacctccccg
     9901 atgcgctcaa gcacagactg caccatctgc attcccgtct gacgttcgcg actgacggct
     9961 tcgttaaatc gagcgttcag caccggaagc tggcctaagg tatccgcaac catcgcttgc
    10021 aggctgccgt ccagtaaatg acgaatatca atcgccatct ctggcagtcc cagctgctgc
    10081 ataatcccgg cggatttatg ttcatagttg atggcaatcg ccggagtagc aaagttcatt
    10141 gagataatgg cggagtgcag gcgcgtaccg acggtgagtt cacaggctcc gagaattttg
    10201 cccatttcca gatcgttgag ttcatccatc actacgtggt aacgggcagg atcgctgatg
    10261 tgctggcgca gattgagcgc caccatgcgg tcgtctttgt tatagctgtc gatacccgta
    10321 caggtggaga gcgcaatcac ctgatagcct tcatcgagaa tgcgattgac caccccggca
    10381 aaggcttttt catacgcttg ttgagtggtg ccgagacgtt tgtcgaacgg tgccagttcg
    10441 cgcagggtaa ttgccactgt tttctgttgt gcggcaacgt ccagccagtg ttgaacggca
    10501 tagctggcgg tgaagtcttc ggtgtggtga tcgaccagcc acgcggtatc gacgccatgt
    10561 tccacttttg cggtggtgat atttctgcgt ttcatcaaat caaggctgac cgattcgcgc
    10621 aggatcagcg cgtcgcagtg accaaagaca tagttcgcca gttggttaaa ttgctcatcc
    10681 tggaaggggc cgacgctgtg accaatcata aacagcggct ttttcgccat aaatgtgcaa
    10741 agcgcatgtt caaactgcgg cacgccgtag agatcgacaa aaaacgatcc gccgacctga
    10801 ataatggcgt cgtaacctga cagcaggcgc acgaagtcgg tgaatccctg ggcgatggcg
    10861 atattgcgca gcttgccagt gtcggtgacg cgtgagagca atacctgatg ctggtagcgg
    10921 cgacggagga cttttttaat gcgcccgaca acgcccgccg cgctattgtg ttgtgtcatc
    10981 tgcaggaaca gcggatcgcc cattaccggg cggttgagca gccaggaaga actgaccgga
    11041 tagcggctca tcacgtccac ttcggtatgt ggattgagga tgttgatggc atcaagtaag
    11101 ccgcgcaaga tggcgctgtc gccacgattg ccgcaagtgt ggttgcccag aataagtaat
    11161 ttcatttttt cctcataaat ttgatgccag gtgaggctgt gtttgttgtg ttcgttgccg
    11221 ttttttgcct gatgcgacgc tggcgcgtct tatcaggcct acaggtcccg agcacagaac
    11281 cgtaggacgg ataaggcgtt ttacgccgca tccggcaacc gttgtcggaa ccgaaaacag
    11341 caattcaccc cgcccgtaaa agcattttca ttttttcgct gcgacaaaac tgacgcttca
    11401 cttccaccac cagcggatgg cgtgacagca caatcatcac cacaaacgcc agcacgcccg
    11461 cggctatttg caccgccagc agcatcccca gcgccagttg ccctttcagc acaatgccca
    11521 gcgcataact gaccaccagc gtcggcaacg agagataaaa cggcagccac aaactcagga
    11581 tgtactggcg ataactggaa ccaagaaccg gtttaatcat cacgaagtaa ctcagaatgg
    11641 tgttgataat ctgcaccagc aggaaaccga gcgtgacgcc gattgcgccc gccatctgcc
    11701 caccaataac aatcgccgga ataaacagaa atgttttgaa tacgttgaat ttaaagctga
    11761 tatcgacccg cgctttcgcc atcagcagcg aaccaatcgg gttacccacg gagcgcagca
    11821 gacccacaat acacagcaat tgcagcaccg gaataatgct gttccacttt tcgccgaata
    11881 ccagcggtac gaagttattc gacaccacca tcagccccag cagcgccgga aagttgataa
    11941 tccccactat cgacagcagc ttgtagaagt taacacgcag cttttcggtg tcgtcctgaa
    12001 ttttggcaaa cgccggaaac aacacgcggg tgatgattgg gttcagcttc atcggcggta
    12061 cgacggccac gttgtacgcc aggttgtatc cccctgccac gcccgcgcca agaatacgcg
    12121 ccagcaccag cgttgaaagg ttggtgttga gatagttgat gatgctgtcc gccgtcagcc
    12181 aggcaccaaa acgtaagttc ggtgccacag acgccagcga gaagtgcaga ccggggcaat
    12241 aaattttgcg tccaaagtag ccaaacagca gtgttctcac cgcactattg accagataac
    12301 cgaggatcgc ggtcatcgcc agcggccaga aatgggcgct aactaccgta aaagtgaagc
    12361 ccgccagcac cgcgctggtt tcgatcatgc cgattttgtt gaactccagc tccttctgca
    12421 tcaacgcgcg gaactgttgc ccgtggggga tcaccacaaa cgccagcgac aatgttttaa
    12481 tcaacggtgc caggtccggg ttattcagca cgtcgccgat gacatcactc aacaaaaaca
    12541 ccgccacaca taccacaagc cccagcccga cgttcagcca gtacagcgtg gtgagttcaa
    12601 ggtgactgat ttcttttcgc tgaataatcg agttagcgat accgaagtca gaaagcgtat
    12661 ctgccagcgc gataatcacc agcgacacag tcagcaggcc gaactggtgg ttgtcgataa
    12721 tccgcgccag cacggtcatc tgcaccagcc cgaggccgat gatgatcacc gtggcaatag
    12781 ccgaccactt cgcgccgctg atggtttttt cacgtaagct catatcaata tgccgctttg
    12841 ttaacgaagc ccttgaatac cgtcaggaaa acgattttga tatcgaacca gacgctccat
    12901 tcgcggatgt actcaaggtc gaactcgacg cgtttttcca ttttctccag cgtgtcggtt
    12961 tcgccgcgcc agccattaat ctgcgcccag ccggtaatgc ccggtttcac tttatggcgc
    13021 agcatgtagc cttcaatgag ctggcgatac tgttcgttat gcgctactgc gtgcggacgt
    13081 ggaccgacaa tcgacatccc cccggtcagc acattgataa actgcggcaa ttcgtccagc
    13141 gaggtgcggc gcagaaagtt ccccacttta gtgacgcgcg gatcgttctg cgtcgcctgg
    13201 gtcaccactt tgtcgttctc catcactttc atggaacgga acttccacac tttgatcggc
    13261 ttgccatcca taccatagcg agtctggcgg aaaataaccg gccctggtga actgagtttc
    13321 accgccagcg caatacagca cagcaccggg gagatcagca gcagaataag cgtcgccagc
    13381 acaatgtctt ccgcacgttt tagcaggcgg ttaacccccg aaagcggcgt gtcatacagc
    13441 ggcaccaccg gaacgccgtt catctcttcg agacgtgaat ggagaatgtt gaaggtaaag
    13501 acgtcgggga tcagcagcac cgaacaggtg gtatccgcca gttgatggac cagttttttc
    13561 actcgtgcgc cgtcgcacat ttgcatcgcg atatagacgt tatgaatctt gcccgctttt
    13621 gcatcctcga ccagctgttg caggttaccc gcccagtcgt tagaaacgcc gcccggtttc
    13681 gggtcgtggt aaacgcccac cacttcaaac cctaaccacg gctgattacg gaaactctcc
    13741 atcagcattt gtccggcggc taaatccccc gctacggcga ccatccgctt gttgtagcca
    13801 tgattacgca gccagcccgc cccaatgcga atacatgaac ggcaaaccac cagtccgatg
    13861 ctggtcagcc cataccacgc cagccagatt ttcagttgcg tgtcgaaatc attgttgaac
    13921 gccaccagtc cggcgctgaa aatcacgctt aaggtccagt tttgtagcaa cagggcaaat
    13981 tctgtcgctg cccgaacacc gcgccatgag cgataaaaat cggtgatgcc gcccagcatc
    14041 tggaacacca ccagcgtaat cagcgccacc aacaggtgca tgtagaggaa tgacagtccg
    14101 ctgacttcgc aaaccagcca tagcccggca aacatgatgg tgatatctga aaagcgttgc
    14161 accatagaga ttaacgatgc attggttttc gctcgctcgc gcttttttag atttgtcatc
    14221 gttgttcctg ttattagccc cttacccgca gtggggtaag ggaagatccg acattactcg
    14281 ttcagcaacg tcagcagagt tcgcgttcgc gcttccatca gcggcacatc accgcgcgat
    14341 tccacattca ggcgcaccac cggttcggta ttggaggtgc gcaggttaaa gcgccagtcg
    14401 gcaaaggtca tgctgatgcc atcgctgcga tccaccgcca gcgcctcacg gctaaaatgc
    14461 tgttccacgc ggttaatcgc ctcaacgggt tgcgccagtt tgctgttgat ctcaccgctt
    14521 gccggaaacg ccgccatccg gtcgcgcacc agttcgccca gcgtttttcc tttcaggcac
    14581 accagttcgg cgaccagcag ccacgggatc atgccgctgt cgcagtaagc gaaatcacgg
    14641 aagtaatggt gggcgctcat ttcgccaccg tagatggcgt cttccttgcg catccgttct
    14701 ttaataaagg cgtgtccggt tttcgacatt accggcgtgc cgcctgcagc agtcaccaca
    14761 tcaatagtgt tccaagagag acgcggatca tggatgatct tcgcgccggg atttttttcg
    14821 aggaacgctt ctgccagcag gccgacaatg tagtagccct cgataaactg ccctttttcg
    14881 tcaaacagaa aacagcggtc aaaatcgcca tcaaaggcaa tgcccatatc cgcgccgtgt
    14941 ttgatgaccg cattgcgggt gtcgtcgcga cattccggca gcagcgggtt aggaataccg
    15001 ttggggaaat tgccgtccgg tgtgttgtgt actttgatta attccacggg cgcgccgagg
    15061 gctttaaagc gggcttcaat ggcgtccacc accggacccg ctgcgccatt cccggagttg
    15121 atcaccagct tgagcggcgt gagatttttg acattgatat aaccgaacag gtgatcaacg
    15181 taagcgtcac gcaggttgat ttgctgatag cgaccgcgtt tggtttcatc gacgggagga
    15241 aagtcgttgg cttcagccag acgctggacg tcgcgcagtc cggtatcacc gctgatcgtg
    15301 cgagccccct cgcgcaccag cttcatgccg ttgtaatcca tcggattatg gctggcagta
    15361 acttcaatgc cgccatccac gccgagatgg aacgtggcga aatagatctc ttcggtgccg
    15421 gacataccga tatccagcac atcgacgccc gcatcctgta aaccttttgc cagcgccagt
    15481 tttaaggttt cgctggtgag acggacatca ccgcctaaca caatggtttt cggtttgaga
    15541 aattcgccat aggcgcgacc aatgcgccag gcgatatctt cattcagttc ttcgcctaat
    15601 ttcccgcgaa tatcataggc tttaaagcag gttaattttt tcatgtcgtt accctttttc
    15661 aggcaatagt tggccctgac cgaaacggcc aattttattt ttgtcgttat ttacaccgcg
    15721 gtttcgcatt cattgcctga tgcgacgctg ccgcgtctta tcaggcctac aaaaccgaac
    15781 cgtaggtcgg ataaggcgtt cacgccgcat ccgacaaaca gcgcctgatg cgacgtttac
    15841 acccgtccgt agcgatccgc gaagcgcacc acatcatcct cttcgagata agagccggag
    15901 cgcacttcaa ttaaatcgag cggaattttc cccgggtttt ccaggcagtg cgtcgccccc
    15961 agcggaatat aaatggactc gttttcacca agcagtttga tatcaccatc aatggtgact
    16021 tttgccgttc ccgcgaccac tacccagtgt tccgcgcggt gatggtgcat ctgtaccgac
    16081 aaaccctcgc ccggtttcac ggtgatgcgt ttcacctggt agcggtcgcc cgcgtcgata
    16141 gagtcatatt tgccccacgg acgatacact tcgcgatgca cccgatgctc atggcgacca
    16201 tcggctttga tctgctcgac cacttttttc acatcctgta ccgcgttacg gtcggcaatc
    16261 agcaccgcat ctttggtctg caccactacc agatctttca ccccgacggt ggtgaccagg
    16321 cctgattcgg catacacata gctgttttca gttttgtgat taatcacatc gccgtggcaa
    16381 acgttgccct cggcggtgtg ggcgctgatc tcccataatg aagaccagga accaacatcg
    16441 ctccagcccg catccatcgg caccacaaca gcatctgccg taggttccat gaccgcgtaa
    16501 tccaccgact cttccgggca ggcgagaaac gcttcttcat ccacacgaat aaaatcgaga
    16561 tccggatcga cggcgctcat cgctttttcg caggcatcga ggatatccgg gcgatatttt
    16621 ttcagttctt cgagatagcg tccggcgcgg aacaggaaca tgccgctgtt ccagtaatat
    16681 tcgccgcttg ctacataggc ctgggcggtt tccagattcg gtttttcgac aaactgcgcc
    16741 acttcaaagg ccaccgtatc ctgctcaccc acagacactt caccgcgacg aatatagcca
    16801 taaccggttt ctggtagatc cggcacaatg ccaaaggtca ccagcttgcc cgcttcggca
    16861 taaggcatgg cattacgcac ggcggcacgg aacgcgtctt catcggcaat cacatgatcc
    16921 gccgccaata ccagcattaa cgggtcgctc tccgggctat gacgttttgc cgccagcgcc
    16981 gccagtgcaa tggcaggtgc agtgttacgc cctgccggtt cgagaataat gttctcggtg
    17041 agtttgttca gttgacgcag ctgttccgcg acaataaagc ggtgctgctc attgcaaatc
    17101 accaccgggc tttcgcactc cacgccgttc aggcggcaga tggtggtttg cagcatggtg
    17161 agatcgcctt tcaggcataa aaactgcttg gggtaaagta cgcgggaaag cggccataaa
    17221 cggctaccgg agccacctgc catcacaact ggatagagtt tcgactgcgc cataattatc
    17281 cccgaatatc atttataaat tgacgtaaca cgttctcttt atcgagcgtg cgttcggcat
    17341 attcacgtgc caccgtgtta tgtttgggca gcaggagcgc ctgacgaatc cccgccacca
    17401 gcgcctcgac cgattccggt tctacgcaaa ccgcaatacc cggaaaggtt tcgcaaagct
    17461 gccccagttc tgtgtgggcc tctgcggtaa tcaccgcgtt accgcctacc gccagaatat
    17521 tggtcagttt cgacggcaat acggcatctg ccgcgccgcg tttttgcacc accagatggc
    17581 aatcgcccat cttcagcagt gcaggtaaag cgtcatacga ttgcagcggg aaaaattgca
    17641 tgttgcgcag tccacgctgc tgcgccattt tttccagccg cgctttgccg ccgccctgcc
    17701 cgacaatggc aaaaatcagc ggttcatcgc gcaggagatc ggcagcttca ataacgtttt
    17761 ccagcccctg cttttcacca atattgccgg agtaaagaat gatttttttg ttatccggca
    17821 ggccaagctg gttacgaagg gcatcaacat cggcgtctgc aacatgctga aaacgggcaa
    17881 tttccgacca gttggggaag aagatgacgt tatccgccgc cacgcctttt tcgatggctt
    17941 tattcatcat cgaacgcgaa atcgtggaaa cgttatcgac gttatgcagt ccgctacgtt
    18001 cgaacgccgt tgccagctgt gccactttgc cgcctttgcc ttttccggca aggcccagcc
    18061 ccagcatggc gtccacttcg taatcctgaa tatgcagcac ggtacgcgca ccagagagct
    18121 tcgccagcag gcgcattccc ggcgtgcaaa acagcgttgg cactacgcca ataatgcgat
    18181 ccggcttcca gcgacgttgc gccatcagtg gaaagaaact gctgacggca aaactgccga
    18241 gatgcaacaa gcgtttcagg gtgctcggct gttttggcac gtacagcggg cagcgccaca
    18301 ccgtggcggc cccttcttct cgtttgtagc gccaggcgga atagttctca cccacctgcc
    18361 attgcgggta gtaaggcggt gcggtaatga cccgcacctc atgaccttgt gccgccaacc
    18421 attccaccat ctcgccggtg tatttgccga tgccggttaa ctccggcgag tagttaatgc
    18481 cgtagaccag tattttcata atccgggtac tccggcacgc ttctcagcga gaaaataggc
    18541 tcggctgtta gcgtgaacat cgttactggc gagcagcgcg tccggcgtca gccagcggta
    18601 atcgtcatgc tgctcatccg gcagtaacag ctcttcttcc gctactctga agcgaaaacc
    18661 gagcaccaca tagtgagtgg tgaaatccgt gccggagaag ttatcgtcat aaaaatgctg
    18721 ccagacaccg taaaactggc ctgctgttat cggcagacgc agccccagtt ccgccatcgt
    18781 cagccgctca aatgcggctt ccagcgtttc gtctttctgc acgcgccctc ccggcacaaa
    18841 ccagtaaccc tgcgccgggc ggttggttct tttgccaagc agaaactcgc cgcgactgtt
    18901 ctcgacaata aagtcgagag agacaagcgg tgtggagcgc actacagtgg caaagtcttc
    18961 ctgacgtaaa aacatcattc cccccgaaag cggtcttgat tctcaaggaa ccactggtaa
    19021 gtgctggcaa gccccgcttc cagtgagatt tcgtgatacc agccaagctg atgcaggcgc
    19081 gtcacatcca gcagtttgcg cggcgtacca tccggtttgc tggcatcaaa aaccacccga
    19141 cctttgtaac ccaccacttt ggcgatggtt tgcgccagct cgcggatagt gcagtcaacg
    19201 cccgtgccga cgttaatgtg cgacagcatc ggttgggtat tctccagcca gacttcatgc
    19261 gccagctcca tgacatgaat gctcgccgcc gccatatcat cgacgtgcag gaattcacgc
    19321 atcggtgtac cgctgcccca taccaccacg tccggcgcat tctgtgccgt cgcctcgtgg
    19381 aagcgacgca gcaatgctgg tatcacatgg gagttactcg ggtggaagtt gtcatgcggc
    19441 ccgtacaggt tggtcggcat gacagtgcgg taatcgcgtc catactggcg gttgtaagat
    19501 tcgcacagtt tgatcccggc gattttggca atggcgtaag gctcgttggt cggctccagc
    19561 gtgccctgca ataactcgct ttctgccatc ggctgttttg ccagtttcgg gtagatgcag
    19621 gacgatccga gaaacatcag tttgttcacg tcgttctgat gcgcggcgtg aatgatgttg
    19681 ctctcaatca tcatgttctg gtagatgaaa tccgccggat aggtgttgtt ggcaacaatg
    19741 ccgcccactt tcgccgccgc cagatagacc tggtcaatgc gttcgctggc aaagaaatca
    19801 tgcacggcgc ggctgtccag caggttcagt tcatcgcggg tacgtaatac cagctccaca
    19861 tcaccgcgct gttcgagctg ccgcttgatg gcagaaccga ccatcccaca atggccagcg
    19921 ataaaaatgc gttgtttact catgcttatg actccagcgc gatcgccacg tcgtagccgt
    19981 gagatttcag cagagagtgt tttttcgccg cttcgaggtc attagccacc atttcagaca
    20041 ccatctctct gagggtgatt tccggtttcc agcccagttt ttcgtgcgct ttggtcgggt
    20101 cgccgagcag cgtttcaact tctgccggac ggaagtaacg cggatcaacg gcgataatca
    20161 catcacccgg tttaacgccc ggcgcgtcat gcccggtgac ggaaaccaca atgcccttct
    20221 cttcaacacc cgtgccttca aagcgcagtt tgatgcccag ctgtgctgcc gccatttcca
    20281 caaactgacg tacggagtac tgaacgccgg tcgcgataac gaaatcttcc ggctgttcct
    20341 gttgcagcat catccactgc atttttacgt agtctttggc gtggccccag tcacgcaggg
    20401 aatccatatt gccgaggtac aggcacgact ccagcccctg ggcgatgttg gcgattgcgc
    20461 gggtgatttt gcgggtaacg aaggtttcgc cgcggcgcgg ggattcatgg ttgaagagaa
    20521 ttccgttaca agcgtacatg ccgtaggatt cgcggtagtt aacggtgatc cagtaggcgt
    20581 acagtttggc gaccgcatac ggagatcgcg ggtagaacgg cgtggtttct ttctgcggaa
    20641 tttcctgcac cagaccatac agttcagagg tggaagcctg atagaaacga gttttctttt
    20701 ccagaccgag gaagcggatc gcctccagca ggcgcagcgt acccatcgcg tcgacgtcag
    20761 cggtatattc tggtgactca aaagagaccg caacgtggct cattgcgcct aggttgtaca
    20821 cttcatccgg ctgtacttca cgcaaaatgc gcgtcaggtt agaggtatca ctcaggtcgc
    20881 cataatgcag atggaatttc gggttgcagg tgtgcggatc ctgataaatg tgatccacgc
    20941 gctcggtgtt gaatgacgat gcgcgacgct taataccatg cacctcgtaa cctttttcca
    21001 gcagaaactc tgccaggtaa gaaccgtctt gtccggttac accggtgatg agagcgactt
    21061 ttgacatgtc ttattcctct gtatttttga atttattcag tttcaacgcg ttcgcgtatc
    21121 accactgcgg gattcccccg gcaaaccaca tttgccggaa gcgatttaaa aacactgctt
    21181 cgtgcaccca cgacggtgcc gtcgccgatt gtgacgcctg gggcaacaaa gacatcggtt
    21241 gccagccagc atttctcgcc aatcacaata ggcgtggcgt taatggtgaa atgttgactt
    21301 gcatggtcgt ggctaccggt gcataaataa cttttttgcg atatcaccga atgtgcgcca
    21361 atggttattt caccgagggt atataaattg acgtcatcgc caacccacgc gtaatcacct
    21421 aaggttaatt tccacggata ggtaattttt actgacggac gaataactac gttttttcct
    21481 atttttgctc cgaataaacg taataaaaaa gcccgccagc gatacaatac ttgtggcgac
    21541 caggcaaata ttgttgcctg tactgcccac cataattgca ctttaatagc gttgccgccc
    21601 cggaaccctt tcggcaccga gaaaccgctt aaatcttgca ttggttttcc ttatattcag
    21661 actttgttat ataaggcttt agtcttacct gtcgtacgtt ggcgtaaatg ccaggataat
    21721 tctgcccaga agccaggtac atgtaatatc tgccgttgga cttttttggc gtcagcac
//
LOCUS       DAEBXN010000066        21620 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.066, whole genome shotgun sequence.
ACCESSION   DAEBXN010000066 DAEBXN010000000
VERSION     DAEBXN010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21620
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.066"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(39..2108)
                     /gene="glyS"
                     /locus_tag="KJL99_002563"
     CDS             complement(39..2108)
                     /gene="glyS"
                     /locus_tag="KJL99_002563"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HBD4437251.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2118..3029)
                     /gene="glyQ"
                     /locus_tag="KJL99_002564"
     CDS             complement(2118..3029)
                     /gene="glyQ"
                     /locus_tag="KJL99_002564"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HBD4437252.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3124..3423)
                     /locus_tag="KJL99_002565"
     CDS             complement(3124..3423)
                     /locus_tag="KJL99_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HBD4437253.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVRVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3598..4593
                     /gene="wecH"
                     /locus_tag="KJL99_002566"
     CDS             3598..4593
                     /gene="wecH"
                     /locus_tag="KJL99_002566"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HBD4437254.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4635..5072)
                     /locus_tag="KJL99_002567"
     CDS             complement(4635..5072)
                     /locus_tag="KJL99_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HBD4437255.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5118..5459)
                     /locus_tag="KJL99_002568"
     CDS             complement(5118..5459)
                     /locus_tag="KJL99_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4437256.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5628..7082)
                     /gene="xylB"
                     /locus_tag="KJL99_002569"
     CDS             complement(5628..7082)
                     /gene="xylB"
                     /locus_tag="KJL99_002569"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HBD4437257.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(7154..8476)
                     /gene="xylA"
                     /locus_tag="KJL99_002570"
                     /pseudo
     CDS             complement(7154..8476)
                     /gene="xylA"
                     /locus_tag="KJL99_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
     gene            8842..9834
                     /gene="xylF"
                     /locus_tag="KJL99_002571"
     CDS             8842..9834
                     /gene="xylF"
                     /locus_tag="KJL99_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HBD4437258.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKKA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            9912..11453
                     /gene="xylG"
                     /locus_tag="KJL99_002572"
     CDS             9912..11453
                     /gene="xylG"
                     /locus_tag="KJL99_002572"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HBD4437259.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRYQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVERELTAIYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     ITQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11431..12612
                     /gene="xylH"
                     /locus_tag="KJL99_002573"
     CDS             11431..12612
                     /gene="xylH"
                     /locus_tag="KJL99_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HBD4437260.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12690..13220
                     /locus_tag="KJL99_002574"
     CDS             12690..13220
                     /locus_tag="KJL99_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBD4437261.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDVVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLAD"
     gene            13332..13868
                     /locus_tag="KJL99_002575"
     CDS             13332..13868
                     /locus_tag="KJL99_002575"
                     /inference="COORDINATES: protein motif:HMM:NF024242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBD4437262.1"
                     /translation="MIGIDNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEE
                     MPLQRILVPPVRVIERRSTDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRS
                     NLEKRFKEEVGETIHAMIHAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKK
                     AYDTTPKEYRDVNSEVML"
     gene            complement(13976..14800)
                     /locus_tag="KJL99_002576"
     CDS             complement(13976..14800)
                     /locus_tag="KJL99_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HBD4437263.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWAPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15120..17149
                     /gene="malS"
                     /locus_tag="KJL99_002577"
                     /pseudo
     CDS             15120..17149
                     /gene="malS"
                     /locus_tag="KJL99_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
     gene            17327..18580
                     /gene="avtA"
                     /locus_tag="KJL99_002578"
     CDS             17327..18580
                     /gene="avtA"
                     /locus_tag="KJL99_002578"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HBD4437264.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEGRPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18731..19204)
                     /locus_tag="KJL99_002579"
     CDS             complement(18731..19204)
                     /locus_tag="KJL99_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBD4437265.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTPVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLQQQPV"
     gene            complement(19306..20154)
                     /gene="yiaJ"
                     /locus_tag="KJL99_002580"
     CDS             complement(19306..20154)
                     /gene="yiaJ"
                     /locus_tag="KJL99_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HBD4437266.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            20354..21346
                     /gene="yiaK"
                     /locus_tag="KJL99_002581"
     CDS             20354..21346
                     /gene="yiaK"
                     /locus_tag="KJL99_002581"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HBD4437267.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRILP
                     MGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPTRD
                     AKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            21358..21504
                     /locus_tag="KJL99_002582"
                     /pseudo
     CDS             21358..21504
                     /locus_tag="KJL99_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
     gene            21561..>21620
                     /locus_tag="KJL99_002583"
     CDS             21561..>21620
                     /locus_tag="KJL99_002583"
                     /inference="COORDINATES: protein motif:HMM:NF015750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4437268.1"
                     /translation="MASISIRCPSCSATEGVVRN"
BASE COUNT         5427 a         5514 c         5307 g         5372 t
ORIGIN      
        1 aagcctgcca gatggcaggc tttttaataa cggcgttatt actgcaacag cgaaatatcc
       61 gcaacgcgca ggaacagttc gcgcagtttc tccagcatgg tcagacggtt gagacgcaat
      121 tctttgtcat caaccatgac catcacttta tcgaagaaag catcaaccgg ctcacgcagc
      181 tctgccagtt cgaccagcgc atcctggtaa cgaccttccg caaagtacgg ctccagcttg
      241 tcacgtagca ccacaacctg catcgccagt ttaatttctt ccggctcttt cagggtagag
      301 gcattcacgc gatcgctcag cacttcgtca gatttcgcca ggatgttaga tacacgtttg
      361 ttcgccgccg ccagtgctgc agctgcttcc agggtacgga agtgcgatac cgctttcatg
      421 cgggcatcga aatcagccgg acgagtcgga cgacgcgcca gtaccgcctg gatggtgtca
      481 acggtataac cttcgtcctg ataccaggcg cggaagcgac cgagcataaa gtcgataaca
      541 tcatcaacta cgttggcatt agtcagctta tcgccataca gacgcaccgc ttcttcggtc
      601 agcgtttgca gatcaaggtt gaggttcttc tcaacgataa ttcgcagcac gccaagtgcg
      661 gcacgacgca gcgcaaacgg gtctttgtcg cctttcggat gctgaccgat accgaagata
      721 cccgccagag tatccatctt atcagcaatc gccagcgcac aagctaccgg gttggacggc
      781 aggtcatcac cggcaaagcg cggctgatac tgctcattca gcgcgacggc tacgtcttcc
      841 gcttcgccat cgtgacgcgc gtagtgcatc cccataacgc cttgggtgtc ggtgaactcg
      901 aagaccatgt tggtcatcag gtcacactta gacagcaggc ccgcacgggt tgcgtggtta
      961 acgtcagcgc caatctgttc ggcgatccag ccagccagcg cctggatgcg gtcagtcttg
     1021 tcacgcagcg tacccagctg ttgctggaac agcacggttt gcaggcgcgg caggttatct
     1081 tccagacgtt ttttgcggtc ggtgttgaag aagaactcgg catccgccag acgtggacga
     1141 acgactttct cgttaccaga gataatttgc tgcggatctt tcgattcgat gttggcaacg
     1201 aagataaagt tcggcagcag tttgccgtcg ttcgcataca ccgggaagta cttctggtca
     1261 cctttcatgg tgtaaaccag ggcttcagcc ggcaccgcga ggaatttctc ttcgaatttt
     1321 gcggtcagaa cgaccggcca ctccaccagc gaagccactt cttccagcag gctttcgctt
     1381 aagtcagcgt taccgccaat cttacgcgct gcttcttcgg catcggcttt aatcttcgct
     1441 ttacgttctt cgtaatcggc gatgactttc ccgcgctcac gcagaatttc cggatactga
     1501 tcggcgttgt cgatggtgaa ttccggttcg cccataaagc ggtggccgcg aatcacgcga
     1561 tcggactgaa tgcccagaat ggttgccgga atgactttgt cgcccagcag cagggtcacg
     1621 gtgtgcaccg gacgcacgaa gtgcacgtcg cttgcacccc agcgcatcag tttcgggatc
     1681 ggcagtttcg ccagcgaagt cgcaaccata ttcggcagca gtgcttcggt gctttcgccc
     1741 ttcacatggg cgcgatacag cagccattcg cctttatcgg tagtcagacg ctcagcctgg
     1801 tcaacggtaa taccacaacc acgcgcccaa ccttctgccg ctttgctcgg ttttccttca
     1861 gcgtcgaacg cctgggcaat cgccgggccg cgtttttcga tttcacgatc tggttgtgct
     1921 tccgccaggt tagctacttt cagcgccaga cgacgcggag cagcaaacca ttgaacggtg
     1981 ccgtgtgcga ggcctgcgtt atccagctcc gcagtaaagt tcgcagcaaa ggactctgcc
     2041 aggctgcgca gtgcttttgg tggcagctct tcagtgccga tttccaccag aaaagttttc
     2101 tcagacatag ccgcctctta cttatctttg ttgcacatcg ggaagccgag ggcttcacgg
     2161 gaagcgtaat atgcttctgc cactgctttg gtcagggtgc gaatgcgcag aatgtagcgc
     2221 tgacgctcgg tgacggagat ggctttacgc gcatccagca ggttgaagct gtgggcggct
     2281 ttcagaatac gctcgtaggc tggcagcggc agcggatttt ccagcgccag cagctgctgc
     2341 gcttctttct cgtactgctc aaagcaggtg aacaggaagt ccacatccgc gtattcgaag
     2401 ttgtaagtgg actgctccac ttcgttctga tggaacacgt cgccgtaggt ggttttaccc
     2461 agcgggccgt cgctccagac taggtcgtaa acgctgtcta cgccctgaat gtacatggcc
     2521 agacgttcca gaccgtaggt gatctcgccg gtaaccggtt tacactccag accaccaacc
     2581 tgctggaagt aagtgaactg cgtcacttcc atgccgttta gccacacttc ccagcccagt
     2641 ccccaggccc ccagagtcgg gttttcccag ttatcttcca cgaaacggat gtcgtgaata
     2701 gtcgggtcca tgcccagctc tttcagagaa ccgaggtaca gctcctgaat attgtccggc
     2761 gatggcttaa tgaccacctg gaactgatag tagtgctgta aacggttggg gttttcgccg
     2821 tagcgaccat cggtcgggcg acgagaaggc tgaacataag cagccgccat cggttctggc
     2881 cccagcgcgc gcagacaggt cattgggtga gaggttcccg cgccgacttc catgtccaat
     2941 ggttgaacaa tggtgcagcc ctggcgagcc cagtaatcct gtaaggtcag gatcaagccc
     3001 tggaaggtcc tggtatcaaa cttttgcata ttatttcgtg ctggatacgt gtggatttaa
     3061 aggaagggat cagtataccc gctggatgga agatatacag tacgaaacgg gaaaaagcag
     3121 ggcttaacgc atggaaagat gcaaaaaatg gccatctgcg tcaaaagaac agacaaactg
     3181 ctctttacgg aaggtataac cgcgcatttc atagcttccc tggaactgtt cgaaggcggt
     3241 gacgtcgatt ttttgctcgc cggtgttata acgttttgcc gcctgatcct tgcataacgc
     3301 ttccatattc aacgaacgca gaggatcaac ccttacccgt tgtgctttct gcacgggtga
     3361 cggtgtagaa caacccacta gcaccataag cgccattgcc ggaaagaaag cgttcatcat
     3421 cattaaatta ccgtctggtt tgcaggtgag tcttattatt tatatcggta tagtggctga
     3481 tatcttacga atttccttaa gcctctatag gctgtagtga taaaactcag attattccag
     3541 tgcgccaacc aaatgattta tcggtcagac tttttttaac cagatacaga gacacagatg
     3601 cagcccaaaa tttactggat tgataacctg cgagggatag cgtgtttaat ggtggtgatg
     3661 attcacacca ctacctggta tgtgaccaat gctcatagtg ttagccccgt cacatgggat
     3721 atcgccaatg ttctgaattc tgcctctcgt gtcagcgtgc cgctattttt catgatttcc
     3781 ggctatctct tttttggcga acgcagcgcc cagccgcgcc atttcttgcg tatcggctta
     3841 tgtctgattt tttatagcgc aatcgcactg ctctacattg cgctgtttac ctccatcaat
     3901 atggagttag cgctgaaaaa cctgctgcaa aagccagtgt tttaccactt gtggtttttc
     3961 ttcgcgattg cggtgattta tctggtttca ccgctgattc aggtgaagaa cgtcggcgga
     4021 aaaatgttgc tggtgctaat ggtggtgatt ggcattatcg ctaacccaaa cacagtgccg
     4081 cagaaaattg acggttttga atggctgcca attaacttat atatcaatgg cgatactttt
     4141 tactacattc tgtatggcat gttgggccgc gctataggga tgatggacac acagcataaa
     4201 gcactgtcgt gggtgagcgc cgcgctgttt gcgacggggg tttttattat ctctcgcggg
     4261 acattatatg aattgcagtg gcgcggaaat tttgccgata cctggtatct ttactgtggg
     4321 ccgatggttt ttatctgcgc aatcgcgcta ttgactctgg ttaaaaacac gctggatacg
     4381 cgtaccattc gcggacttgg cttaatctcc cgccattcat tgggtatata cggattccac
     4441 gccttgatta tccatgcgct gcgcacccgg ggaattgagc ttaaaaattg gccaatactg
     4501 gatattattt ggatcttttg cgcgacgttg gcagcgagtt tgttactttc tatgctggta
     4561 caacgaatcg acagaaacag attagtgagt taagtaaaag cccgttcaca ttggactgac
     4621 cgggcttacg tgaattattc gctgtattct tcctgggtaa cctgtgtttc ttttggcggg
     4681 tttattccgg catcacgaat attcttctgc accgctactg caccaaacaa gcttaagaag
     4741 aaagccagtc cataaaaacc tttttcactg agtaataatg tcgcattcca cagacctacc
     4801 atcagtagag caacagagat aataaagaca gtcaggcagg tcatataata aatggaagtg
     4861 gtcggtatgc cttcatactt gtcccgaacg gtcttttgat aagacgcagc agaaaacagt
     4921 cctaacacca gtacggcaaa ataataccct ttttcattta actgcatctc tgcattccat
     4981 agccctaaca ggtaggtaac gataccacca acgagagcta tccatgacac aatactaaag
     5041 gccggtgaat aggttgatat tttgttgtcc atcacagtat tcctttcatt cctgaatgtg
     5101 taagagcttt atgttgctca taccgagcga aacaaacgca tttgaccaaa caagcagaca
     5161 aaaaaggcga tggggtagat caccatttca taagcattga taggtgcatt taaaacaccg
     5221 ataaacaaga gaccgatagt gattaacgca actaactggc agatatgggt aaaaaaatta
     5281 tccagttgcc cgagtttttc tgcgcgaaga tatgcatagt taccaaaagt tgctgtcatt
     5341 aacacgccaa gaaaatagcc ttttccactt agcaatgggc atgagatcca tagcccaacc
     5401 agataaacca gcgccccgac aacaaataat agttttgcca cagtttttga ggttttcata
     5461 acgatctcca tatctaccag cgatacatta cgagtaacca acgaaagaca aaactgaaaa
     5521 atgccattaa caaatgattt tcagaataaa ttcatactaa atattaatta attagtgaga
     5581 tatatagatg tgaattatcc cccacccggt caggcagggg ataacgttta cgccattaat
     5641 ggcagaagtt gctgatagag gcgacggaac gtttcacgtc gtggctgata agcggcataa
     5701 cgctgcgcat ctggtagatg cgactgttct aacggtagct gcggcaacaa ctcaatgagc
     5761 gatttctctg gattcgccgc aatctgcgcc agccttgctg cgcccagtgc tggccccaca
     5821 tccccccccg tacggtaatc gagctgctga ccgctgatat ccgccagcat ctgacgccag
     5881 tactcactac gcgccccgcc accaatcaac gtaacacttt gcggtttaat gccgcaggca
     5941 tgcacgacat ccatgccatc tgccagcgca taacccacgc cttccagcac tgctcgcgcc
     6001 agttcattgg ggccatgttg atgagtcaaa ccaaagaaaa cccccttcgc ctggggatta
     6061 ttgtgtggtg tacgctcgcc ggaaagataa ggcagaaacc aaactggctc ggcactttca
     6121 tcagcctgtt gagctgcagc gattaaagct gggacattgc acaggccggt taatttcgcg
     6181 gcccaatcca gacacgatgc tgcactcagc attacagaca ttaaatgcca acgctgcggt
     6241 agcgcatggc aaaagctgtg tacagcgctt tctggcttgc ttaagaaccc ttcgctgaca
     6301 gcaaaataga cccccgacgt ccccagcgat aacattgcct gattagcatc aaccattccc
     6361 acaccaactg caccagctgc attgtcgcca ccgcctgcga caactggcac cgtcgccata
     6421 ccccacgctt tcgcaacttc aggtaacaaa gcaccagtaa tttcgctgcc ttcgtataat
     6481 gcgggcatct ggtcacgaga taagtcgcaa gcctgcagca tgacgtcact ccagtcacgc
     6541 tttgcgacat ccagccacat ggtgccagct gcgtcagaca tatcgctggc aaactccccc
     6601 gtcatacgca gacgcaagta atctttcggt aataatactt tgtcgatttg acggaatatc
     6661 tccggctcat gccgctgaac ccatagcaat ttaggcgcag taaatccggg catcatcagg
     6721 ttgccggtaa tcactcgtga ttgcggaact ctcgcttcca gcaaagtgca ctcttgcgca
     6781 cagcgcccgt cgttccacaa aatggcaggg cgcaataccc gttgttgagc atccagtaag
     6841 gttgctccat gcatctggcc ggcaataccc aatgctttaa cgtcctgcag agaatgctga
     6901 tcgcccagag ctttcattgc gcgatcagtt gcctgccacc actgttccgg gtcttgttcc
     6961 gaccaaagtg gatgcgggcg cgaaacggtc agcttttccg tttgcgaagc aaccacctca
     7021 ccctgctcgt tgagcaaaat aacttttacg cccgaggtgc caagatctat cccgatatac
     7081 atatcgatcg ttccttaaaa aaatgcccgg tatcgctacc gataaccggg ccaacggact
     7141 gcacagttag ccgttatttg tcgaacagat aatgatttac cagattttcc agttgctcct
     7201 ggcgaccact ctgatgcacc ggagacaaat tatgttcctg agcatatttg gctaaatctg
     7261 ccagtgacat ttggcctttc aggatttgct ggcccaattc gctattccag ccggaataac
     7321 gctgcgcgat gcgtttatcc agctcgccat cttcaatcat gcgcgctgca attttcagcg
     7381 ccagtgccat cgtatccatc gcgccgatat gaccgtaaaa cagatcatat ttatcagtac
     7441 tttgacgacg tactttggca tcgaagttca gaccaccggt ggtgaaaccg cctgctttga
     7501 gaatttcata catcaccagc gcattctctt ccacactgtt cgggaactgg tcggtgtccc
     7561 agcccagttg cgcatcgcca cggttggcgt cgacagaacc gaacaggcca agcgcaatgg
     7621 cggtggctat ttcatgatgg aaagagtgac ctgccagcgt cgcgtggtta gcttcaatgt
     7681 tcagtttaat ctctttttcc agaccaaact gtttcaggaa gccatagacc gtcgcggcat
     7741 cgtaatcata ttgatgttta gtcggttctt gcggtttcgg ttcgataagc aacgtgccct
     7801 agaagccgat tttatgttta tgctcaacca ccatctgcat aaagcggccc agttgttcac
     7861 gctcctgacg caagtcggta tttaacagcg tttcgtaacc ttcacgacca ccccacagga
     7921 catagttttc accgcccaat ttatgggttg cttccatcgc tgtaacaact tgcgttgccg
     7981 cccagctgaa gacttcagga tctgggttcg tcgccgcacc cgcgccatag cgagggtttg
     8041 taaagcagtt agcggttccc cacagcagct tcacgccgct ctcttcttgc ttggctgcca
     8101 ggacatcaac catttgcgca aaattattga tgtactcttt taacgacgcg ccctcagggg
     8161 aaacatccac atcgtggaag caataaaatg gcacatgtaa cttgtggaaa aactcaaatg
     8221 cgacatctgc tttacgcttc gccaacgcca gtgcctcacc aggctgctgc cacggacgat
     8281 taaacgcccc cacaccaaac atgtccgccc cgttccagca gaaggtgtgc cagtagcagg
     8341 cggcaaaacg caagtgctct tccatacgct tacccaacac cagttcgtcg ggattgtagt
     8401 gacggaatgc taacgggttt gaggattttg agccttcata acgaacgcga tcgagctggt
     8461 caaaataggc ttgcatattg aactccataa tcaggtaatg ccgcgggtga tggatgatgt
     8521 cgtaatattg ggcactccct ttcaattgct caattatgtt atttcacact gctattgaga
     8581 taattcacaa gtgtgcgctc gctcgcaaaa taaaatggga tgatgaaaat gggtaattcc
     8641 tcgaagataa aaatgcaata agtacaattg cgcaacaaaa gtaagatctc ggtcataaat
     8701 caagaaataa accaaaaatc gtaatcgaaa gataaaaatc tgtaattgtt ttcccctgtt
     8761 tagttgctaa aaattggtta cgtttatcgc ggtgattgtt acttattaaa gctgtcctct
     8821 aactacagaa ggccctacac catgaaaata aagaacattc tactcaccct ttgcacctca
     8881 cttctgctta ccaacgttgc ggcacacgcc aaagaagtca aaataggtat ggcgattgat
     8941 gatctccgtc ttgaacgctg gcaaaaagat cgagatattt ttgtgaaaaa ggcagaatct
     9001 ctcggtgcga aagtatttgt acagtctgca aatggcaatg aagaaacaca aatgtcgcag
     9061 attgaaaaca tgattaaccg gggtgtcgat gttcttgtca ttattccgta taacggtcag
     9121 gtattaagta acgttgtaaa aaaagccaaa caagaaggta ttaaagtatt agcttacgac
     9181 cgtatgatta acgatgcgga tatcgatttt tatatttctt tcgataacga aaaagtcggt
     9241 gaactgcagg caaaagcctt ggtcgatatt gttccgcaag gtaattactt cctgatgggc
     9301 ggctcgccgg tagataacaa cgccaagctg ttccgcgccg gacaaatgaa agtgttaaaa
     9361 ccttacgttg attctggaaa aattaaagtc gttggtgacc aatgggttga tggctggtta
     9421 ccggaaaacg cattgaaaat tatggaaaac gcgctaaccg ccaataataa caaaattgat
     9481 gctgtagttg cctcaaacga tgccaccgca ggtggggcaa ttcaggcatt aagcgcgcaa
     9541 ggtttatcag ggaaagtagc aatttccggc caggatgcgg atctcgcagg tattaaacgt
     9601 attgctgccg gtacgcaaac tatgacggtg tataaaccta ttacattgct ggcaaatact
     9661 gccgcagaaa ttgccgttga attgggcaat ggtcaggagc caaaagcaga tacctcactg
     9721 aataatggcc tgaaagatgt cccctcccgc ctcctgacac cgatcgatgt gaataaaaac
     9781 aacatcaaag atacggtaat taaagacgga ttccacaaag agagcgagct gtaagcgtta
     9841 cgccccggcg cggagcgggg gcgtgatttc tctccatgcc gcgtgaatga attggcttag
     9901 gtggagtcgt tatgccttat ctacttgaaa tgaagaacat taccaaaacc ttcggcagtg
     9961 tgaaggcgat tgataacgtc agcttgcggt tgaatgctgg cgaaatcgtc tcactctgtg
    10021 gggaaaatgg gtctggtaaa tcaacgctga tgaaagtgct gtgtggtatt tatccccatg
    10081 gctcctacga aggcgaaatt atttttgcgg gagaagagat tcaggcgagt cacatccgcg
    10141 ataccgaacg caaaggtatc gccatcattc accaggaatt ggccctggtg aaagaattga
    10201 ccgtgctgga aaatatcttc ctgggtaacg aaataaccca caatggcatt atggattatg
    10261 acctgatgac gctacgctat cagaagctgc tcgcacaggt cagtttatcc atttcacctg
    10321 atacccgcgt tggcgattta gggcttgggc aacaacaact ggttgaaatt gccaaggcac
    10381 ttaataaaca ggtgcgcttg ttaattctcg atgaaccgac agcctcatta actgagcagg
    10441 aaacgtcggt tttactggat attattcgcg atctacaaca gcacggtatc gcctgtattt
    10501 atatttcgca caaactcaac gaagtcaaag cgatttccga tacgatttgc gttattcgcg
    10561 acggacagca cattggtacg cgtgatgctg ccggaatgag tgaagacgat attatcacca
    10621 tgatggtcga gcgagagtta accgcgattt accctaatga accacatacc accggagatg
    10681 aaatattacg tattgaacat ctgacggcat ggcatccggt caatcgtcat attaaacgag
    10741 ttaatgatgt ctcgttttcc ctgaaacgtg gcgaaatact gggtattgcc ggactcgttg
    10801 gtgccggacg taccgagacc attcagtgcc tgtttggcgt gtggcccgga caatgggaag
    10861 gaaaaattta tattgatggc aaacaggtag atattcgtaa ctgtcagcaa gccatcaccc
    10921 aggggattgc gatggtaccc gaagacagaa agcgcgacgg catcgttccg gtaatggcgg
    10981 ttggtaaaaa tattaccctc gccgcactca ataaatttac cggtggcatt agccagcttg
    11041 atgacgcggc agagcaaaaa tgtattctgg aatcaattca gcaactcaaa gttaaaacgt
    11101 cgtcccccga ccttgctatt ggacgtttga gcggcggcaa tcagcaaaaa gcgatcctcg
    11161 cccgctgtct gttacttaac ccgcgcatcc tcattcttga tgaacctacc aggggtattg
    11221 atattggcgc gaaatatgag atctacaaat taattaacca actcgtccag cagggtattg
    11281 ccgttattgt catctcttcc gaattacctg aagtgctcgg ccttagcgat cgtgtactgg
    11341 tgatgcatga agggaaacta aaagccaacc tgataaatca taacctgact caggagcagg
    11401 tgatggaagc cgcattgagg agcgaacatc atgtcgaaaa gcaatccgtc tgaagtgaaa
    11461 ttggccgtac cgacatccgg tggcttttct gggctgaaat cactgaattt gcaggtcttc
    11521 gtgatgattg cagctatcat cgcaatcatg ctgttcttta cctggaccac cgatggtgcc
    11581 tacttaagcg cccgtaacgt ctccaacctg ttacgccaga ccgcgattac cggcatcctc
    11641 gcggtaggaa tggtgttcgt cataatttct gctgaaatcg acctttccgt cggctcaatg
    11701 atggggctat taggtggcgt cgcggcgatt tgtgacgtct ggttaggctg gcctttgcca
    11761 cttaccatca ttgtgacgtt ggttctggga ctgcttctcg gtgcctggaa cggatggtgg
    11821 gtcgcgtacc gcaaagtccc ttcatttatt gtcaccctcg cgggcatgtt ggcatttcgc
    11881 ggcatactca ttggcatcac caacggcacg actgtatccc ccaccagcgc cgcgatgtca
    11941 caaattgggc aaagctatct ccccgccagt accggcttca tcattggcgc gcttggctta
    12001 atggcttttg ttggttggca atggcgcgga agaatgcgcc gtcaggcttt gggtttacag
    12061 tctccggcct ctaccgcagt agtcggtcgc caagctttaa ccgctatcat cgtattaggc
    12121 gcaatctggc tgttgaatga ttaccgtggc gttcccactc ctgttctgct gctgacgttg
    12181 ctgttactcg gcggaatgtt tatggcaacg cggacggcat ttggacgacg catttatgcc
    12241 atcggcggca atctggaagc agcacgtctc tccgggatta acgttgaacg caccaaactt
    12301 gccgtgttcg cgattaacgg attaatggta gccatcgccg gattaatcct tagttctcga
    12361 cttggcgctg gttcaccttc tgcgggaaat atcgccgaac tggacgcaat tgcagcatgc
    12421 gtgattggcg gcaccagcct ggctggcggt gtgggaagcg ttgccggagc agtaatgggg
    12481 gcatttatca tggcttcact ggataacggc atgagtatga tggatgtacc gaccttctgg
    12541 cagtatatcg ttaaaggtgc gattctgttg ctggcagtat ggatggactc cgcaaccaaa
    12601 cgccgttctt gattttgata aaaattttct caaagccggt tacgtattac cggttttgag
    12661 tttttgcatg attcagcagg aaaagaacca tgtttactaa acgtcaccgc atcacattac
    12721 tgttcaatgc caataaagcc tatgaccggc aggtagtaga aggcgtaggg gaatatttac
    12781 aggcatcaca atcagaatgg gatattttca ttgaagaaga tttccgcgcc cgcattgata
    12841 aaatcaagga ctggttagga gatgtcgtca ttgccgactt cgacgacaaa cagatcgagc
    12901 aagcgctggc tgatgtcgac gtccccattg ttggggttgg cggctcgtat caccttgcag
    12961 aaagttaccc acccgttcat tacattgcca ccgataacta tgcgctggtt gaaagcgcat
    13021 ttttgcattt aaaagagaaa ggcgttaacc gctttgcttt ttatggtctt ccggaatcaa
    13081 gcggcaaacg ttgggccact gaacgcgaat atgcatttcg tcagcttgtc gccgaagaaa
    13141 agtatcgcgg agtggtttat caggggttag aaaccgcgcc agagaactgg caacacgcgc
    13201 aaaatcggct ggcagactga ctacaaacgc tgccaccgca aaccgggatt attgccgtta
    13261 ctgacgcccg agcgcggcat attctgcaag tatgtgaaca tctacatatt cccgtaccgg
    13321 aaaaattatg cgtgattggc atcgataacg aagaactgac ccgctatctg tcgcgtgtcg
    13381 ccctttcttc ggtcgctcag ggcgcgcggc aaatgggcta tcaggcggca aaactgttgc
    13441 atcgattatt agataaagaa gaaatgccgc tacagcgaat tttggtccca ccagttcgcg
    13501 tcattgaacg gcgctcaaca gattatcgct cgctgaccga tcccgccgtt attcaggcca
    13561 tgcattacat tcgtaatcac gcctgtaaag ggattaaagt ggatcaggta ctggatgcgg
    13621 tcgggatctc gcgctccaat cttgagaagc gttttaaaga agaggtgggt gaaaccatcc
    13681 atgcaatgat tcatgccgag aagctggaga aagcgcgcag tctgctgatt tcaaccacct
    13741 tgtcgatcaa tgagatatcg caaatgtgcg gttatccatc gctgcaatat ttctactctg
    13801 tttttaaaaa agcatatgac acaacgccaa aagagtatcg cgatgtaaat agcgaggtca
    13861 tgttgtagcg atgatgagaa ttgtcggcgt cacatcaggg aactcagcac agccacctga
    13921 tgcgagcaaa agccttatcc gaccgacaaa tcagataagg ctcgaggaaa tgaaattaca
    13981 tatgcgccgc gattaaccgt tggttatcct ggtacattgc gaacaggtag ttgttataac
    14041 tcttcccttt ggtcgagtag cccttcagct tgtgaatcat cgctgtggca gtcacttcct
    14101 gatccgcttt acgcagctgt gcacgcgatt tacggaacga agagtaagcc gggtgcgtat
    14161 tcaggttagt gacataggcg ctcaccgatt ctttgacaga actaaactgt gagtaccctt
    14221 tcactttacc cggcgcattg gtacaacgtc ctttcatgca tttcatgccg aacagattgt
    14281 tattgttgcg cgccagcttc gacgttcccc aaccgctttc tgctgcagcc atcgtcgcca
    14341 ccatactggt agggataatg tctacgcgtt caagcaaggt attccacggg atttttcgcg
    14401 tattaccaga ccacttcacc ttgtagcgtt tggcgatgtc tttcagacgc gcacgctcag
    14461 caggtgccca ttggccctga tactgttttg aaatcagcca gttacgttct gcagtaatgg
    14521 ctgcattttg gctggtaatg taaggcatta cggtccggag aaacgccttt ttccttggtg
    14581 ttccggaagg gtattttcgc aaatcaggaa gtgaactact ctttgcacta ttgcgagaat
    14641 actcttgttt actgcttacc tgtttattac tagctttagt taagtgggac ttttgactcg
    14701 ctgttgttgt gtgcgtcttc gctaacacct cactcgaaaa caccagagtg agtaacataa
    14761 gaatcatcgc cccatatcgt cgtatgggag tcaaaatcat caggtctcct ggtcggattt
    14821 aatcattcca acaccttata tttttcacaa atttgagagt tgaatctcaa atcatatcaa
    14881 aaatagctgt caagagcacc ccaaggaata gtccaaatct gaaactatgt cacgagttaa
    14941 cgattcagat tggcgctaaa tcgcagaaaa tgtgggggtt atcgcaaaat tcagccgttt
    15001 tttgcgcgcg atcgctcacc cttgcttctc atcctgtgga cttaccgctc agggatgagt
    15061 tttgtctggc ttatcgctgg caaactgtct gaaatcgcag caataaggac tcatccacca
    15121 tgaaactcgc cgcctgtttt ctgacactcc ttcctggctt cgccgttgcc gccagctgga
    15181 cttctccggg gttccctgcc tttagcgaac agggaacggg aacatttgtc agccacgcgc
    15241 agttgcccaa aggtacgcgt ccactcacgc taaattttga ccaacagtgc tggcagcctg
    15301 cagatgcgat aaaactcaat cagatgcttt ccctgcaacc ttgtagcaac acgccgcctc
    15361 aatggcgatt gttcagggac ggcaaatata cgctgcaaat agacacccgc tccggtacgc
    15421 caacattgat gatttccatc cagaacgcgc cgaaccggta gcaaacctgg tccgtgaatg
    15481 cccgaaatgg gatggattac cgctcacgct ggatgtcagc gccactttcc cggaaggagc
    15541 cgccgtacgg gattattaca gccaacaaat tgcgatagtg aagaacggtc aaataacgtt
    15601 acaacccgct gctaccagca acggtttact cctgctggaa cgggcagaaa ctgacacatc
    15661 cgcccctttc gactggcata acgccacggt ttactttgtg ctgacagatc gtttcgaaaa
    15721 cggcgatccc agtaatgacc agagttacgg acgtcataaa gacggtatgg cggaaattgg
    15781 cacttttcac ggcggcgatt tacgcggcct gaccaacaaa ctggattacc tccagcagtt
    15841 gggcgttaat gctttatgga taagcgcccc atttgagcaa attcacggct gggtcggcgg
    15901 tggtacaaaa ggcgatttcc cgcattatgc ctaccacggt tattacacac aggactggac
    15961 gaatcttgat gccaatatgg gcaacgaagc cgatctacgg acgctggttg atagcgcaca
    16021 tcagcgcggt attcgtattc tctttgatgt cgtgatgaac cacaccagct atgccacgct
    16081 ggcggatatg caggagtatc agtttggcgc gttatatctt tctggtgacg aagtgaaaaa
    16141 aacgctgggt gaacgctgga gcgactggaa acctgccgcc ggacaaacct ggcatagctt
    16201 taacgattac attaatttca gcgacaaaac aggctgggat aaatggtggg gaaaaaactg
    16261 gatcagaacg gatatcggcg attacgacaa tcctggattc gacgatctca ctatgtcgct
    16321 ggcctttttg ccggatatca aaaccgaatc aactaccgct tctggtctgc cggtgttcta
    16381 taaaaacaaa acggatactc acgctaaagt catcgaaggc tttacacctc gcgattactt
    16441 aacccactgg ttaagtcagt gggtccgcga ctatgggatt gatggttttc gggtcgatac
    16501 cgccaaacat gttgagttgc ccgcctggca gcaactgaaa accgaagcca gctccgcgct
    16561 tcgcgaatgg aaaaaaacta accccgacaa agcattagat gacaaacctt tctggatgac
    16621 cggtgaagcc tggggccacg gcgtgatgca aagtgactac tatcgccacg gcttcgatgc
    16681 gatgatcaat ttcgattatc aggagcaggc ggcgaaagca gtcgattgtc tggcgcagat
    16741 ggatacgacc tggcagcaaa tggcggagaa attgcagggt ttcaacgtgt tgagctacct
    16801 ctcgtcgcat gatacccgtc tgttccgtga agggggcgac aaagcagcag agttattact
    16861 attagcgcca ggcgcggtac aaatctttta tggcgatgaa tcctcgcgtc cgttcggtcc
    16921 tacaggttct gatccgctgc aaggtacacg ttcggatatg aactggcagg atgttagcgg
    16981 taaatctgcc gccaacgtcg cgcactggca gaaaatcagc cagttccgcg cccgccatcc
    17041 cgcaattggc gcgggcaaac aaacgacact ttcgctgaag cagggctacg gctttgttcg
    17101 tgagcatggc gacgataaag tgctggtcat ctgggctggg caacagtgac ttttccggct
    17161 tcccgttcgt cagtacctcg ggaagccgcc aaccaggata aaatgtcagc cctaatcagc
    17221 gttgcgggat aaagcaccgc tcactcttca acagaccgat ttgcacccca gcaaatgtag
    17281 cgttattgtt accttccttg ctacagagtt cgacagatat cccgctatga cattctccct
    17341 ttttggtgac aaatttaccc gccactccgg cattacgctg ctgatggaag atctgaacga
    17401 cggtttacgc acgcctggcg cgattatgct cggcggcggt aatccggcgc agatcccgga
    17461 aatgcaggac tacttccaga cgctactgac cgacatgctg gaaagtggca aagcgactga
    17521 tgcactgtgt aactacgacg gtccacaggg gaaaacggag ctactcacac tgcttgccgg
    17581 aatgctgcgc gagaagttgg gttgggatat cgaaccacag aatattgcac taacaaacgg
    17641 cagccagagc gcgtttttct acttatttaa cctgtttgcc ggacgccgtg ccgatggtcg
    17701 ggtcaaaaaa gtgctgttcc cgctggcacc ggaatacatt ggctacgctg acgccggact
    17761 ggaagaagat ctgtttgtct ctgcgcgtcc gaatattgaa ctgctgccgg aaggccagtt
    17821 taaataccac gtcgattttg agcatctgca tattggcgaa gaaaccggga tgatttgcgt
    17881 ctcccggccg acgaatccaa caggtaatgt gattactgac gaagagttgc tgaagcttga
    17941 cgcgctggcg aatcaacacg gcattccgct ggtgattgat aacgcttatg gcgtcccgtt
    18001 cccgggtatc atcttcagtg aagggcgccc gctatggaat ccgaatatcg tactgtgcat
    18061 gagtctttcc aagctggggc tgcctggctc ccgctgcggc attatcatcg ccaatgaaaa
    18121 aatcatcact gccatcacca atatgaacgg cattatcagc ctggcacctg gcggtattgg
    18181 tccggcgatg atgtgtgaaa tgattaagcg taacgatctg ctgcgcctgt ctgaaacagt
    18241 catcaaaccg ttttactacc agcgtgttca ggaaactatc gccatcattc gccgctattt
    18301 accggaaaat cgctgcctga ttcataaacc ggaaggagcc attttcctct ggctatggtt
    18361 taaggatttg cccattacga ccgagcagct ctatcagcgc ctgaaagcac gcggcgtgct
    18421 gatggtgccg gggcacaact tcttcccggg actggataaa ccgtggccgc atacgcatca
    18481 atgtatgcgc atgaactacg taccagagcc ggagaaaatt gaggcggggg tgaagattct
    18541 ggcggaagag atagaaagag cctgggctga aagtcactaa aaattgcctg atgcgctacg
    18601 cttatcaggc ctacatgatt tctgcaatat attgaatttg gaagaatttg taggccggat
    18661 aaggcgttta cgccgcatct ggcataaaca aagcgcacta ttccggcgtt gagaaacgcc
    18721 ggaaaacgtt tcaaacaggc tgctgctgta gccgatgccg ctgcactttc tcgacatcca
    18781 tgcactgcaa cgcctttgtc gggcaggctt caacacaggc cggccccgtc tcccgatgcc
    18841 agcacagatc gcacttaatc gccctcgcct ttttgcgcga agaaacgacc tccatcgcac
    18901 caaacgggca agccagcata cagcttttac agccaatgca acgtgtttgt tcaacgaaaa
    18961 tatgcccatg ttcgcggctt atcgcgtcaa cagggcagac attcgcgcac ggtgcatctt
    19021 cacactgatg acaggctact ggcgtggtcc agctgtggtc tttaatgaca cgaatacggg
    19081 aaataaactc gtctggtgac aacgcagcgc aatcctgatt ctcctgatgc gacactgcgc
    19141 aagccacttc acaggtacgg caaccgatac atttcgtcgc atccgcaata ataaaccggt
    19201 tcatgatcat tcctttgcac taaaacagca gagtgccaga gagcgcgtcc ctactgcttt
    19261 gatccaacga aggaaatgtt aaagttttgg cacttgtcca aagcgttatg tgatcgcgcc
    19321 caggtcatcc ctgacagtaa atcccagttc attagaaata gcctgcgcgg tttcacgcag
    19381 tggtttcagg agatttttct ctcccacctg tttcagacgt gatgtcgaaa gcgaaatcga
    19441 cacggcgtac ggcacccgcc catgaatatc aaacaccgga acagcaatac aggagacgcc
    19501 gagttcgttt tcttctctgt ccatcgccgc tccgctttca cgaatgtgcg ccagttcgtc
    19561 gaacatcgcg ggcagctcgg taatggtatt gcgggttaac ggctggatct catgctgatg
    19621 gttttcccag tatgacttca cgtagtccgg gtgaccaaac gccatgtaga tcttgcccat
    19681 tgcggaacag tagagcggca tatgctggcc aatataggca cgggttcgca gcatcccggt
    19741 tgtgggttcc agcttataaa tcaaaatagc gtgatcgtct tcgcggctgg agaagttaat
    19801 ggtttcacca gtggcgatgt tcagtgcctc aagatgcgga gcggcgatat gaatgatatt
    19861 cagcgaagac agcgccttct gcccgacggc aataaatttg gtagtcaggc ggtaactccc
    19921 tgcggcgggc gcggtggtca catagccgca ggactgtaat ccctgcaata agcgatggac
    19981 ggtactctta tttaaaccag ccagctccga aagatgcgcc aacggacaac cgtttggata
    20041 gttgctcaaa atctcaatca gcatcaaccc gcgaaacaga ctctggcttc cggctggacg
    20101 ctctttttcc tgcgccatct cgttctcttt ttttcccatc acttctttcc ccattttgtc
    20161 gcgtcctgat ggtagcgcaa agtgtgccgt agttcacgat ctcgacagat aatttataac
    20221 cacttgattt ttatgtcttt tgaaattcat caatcagatt gctttgttaa aaagtgatcg
    20281 atatatttga aatctagttc cgcatattga aattttaagc caaaaaagcg atcaaaaaac
    20341 aaggaagcct aggatgaaag tgacatttga gcagttaaaa gcagccttta atcgggtctt
    20401 aatttcacgc ggcgttgaca gcgaaacggc tgacgcctgt gcagagatgt tcgcccgcac
    20461 caccgaatcc ggcgtttatt ctcacggcgt taatcgtttc cctcgtttca ttcaacaact
    20521 ggaaaacggc gatatcattc ctgatgccca acccaaacgt ataaccagcc tcggcgcaat
    20581 tgaacagtgg gatgcccagc gttcgatcgg caacctgacg gcgaaaaaga tgatggatcg
    20641 cgccattgaa ctggctgccg atcacggtat tggtctggtg gcacttcgta atgccaacca
    20701 ctggatgcgc ggcggcagct acggctggca ggcggcggaa aaaggctata ttggcatttg
    20761 ctggaccaac tccatcgccg taatgccgcc gtggggcgca aaagagtgtc gcatcggcac
    20821 taacccgctg atcgtcgcca ttccttccac gccgatcacc atggtcgata tgtcgatgtt
    20881 ctcttacggc atgttagaag ttaaccgcct ggcaggccgt cagctcccgg tcgatggtgg
    20941 ctttgatgat gagggcaatt tgaccaaaga acctggcgtt atcgagaaga atcgccgcat
    21001 tttgccgatg ggctactgga aaggttctgg catgtcgatt gtgctggata tgatcgctac
    21061 tctcctttcc gacggcgcat cggttgccga agtcacccag gacaacagcg acgaatacgg
    21121 catttcacaa atttttattg ccattgaagt ggacaagctt atcgacggtc ccacccgcga
    21181 tgccaagctg caacgtatca tggattacgt tactaccgct gaacgcgctg acgaaaacca
    21241 ggccatccgc ttacccggcc acgaatttac taccctgctg gccgaaaacc gccgtaacgg
    21301 catcactgtt gatgacagcg tgtgggccaa aatccaggcg ttatgaggag ataagtcatg
    21361 atttttggac atatcgcgca gcccaatccg tgccgtttgc ccgccgccat tgaaaaggcg
    21421 ctcgattttc tgcgcgctac caacttcaac gtcctggagc cgggcgttgt cgaaatcgac
    21481 ggcaagaata tttatgcgca aattaggtaa tgctgccaac ttactgattt agtgtatgat
    21541 ggtgatttta aggtgcttgc gtggcttcca tttccatcag atgtccttcc tgctccgcta
    21601 ctgaaggcgt ggtgcgtaac
//
LOCUS       DAEBXN010000067        21532 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.067, whole genome shotgun sequence.
ACCESSION   DAEBXN010000067 DAEBXN010000000
VERSION     DAEBXN010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21532
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.067"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(19..1017)
                     /gene="fbp"
                     /locus_tag="KJL99_002584"
     CDS             complement(19..1017)
                     /gene="fbp"
                     /locus_tag="KJL99_002584"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HBD4437269.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            1193..2566
                     /gene="mpl"
                     /locus_tag="KJL99_002585"
     CDS             1193..2566
                     /gene="mpl"
                     /locus_tag="KJL99_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HBD4437270.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(2649..3200)
                     /locus_tag="KJL99_002586"
     CDS             complement(2649..3200)
                     /locus_tag="KJL99_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HBD4437271.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRPAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            3294..4646
                     /gene="pmbA"
                     /locus_tag="KJL99_002587"
     CDS             3294..4646
                     /gene="pmbA"
                     /locus_tag="KJL99_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HBD4437272.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            4829..5211
                     /gene="cybC"
                     /locus_tag="KJL99_002588"
                     /pseudo
     CDS             4829..5211
                     /gene="cybC"
                     /locus_tag="KJL99_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
     gene            complement(5256..5720)
                     /gene="nrdG"
                     /locus_tag="KJL99_002589"
     CDS             complement(5256..5720)
                     /gene="nrdG"
                     /locus_tag="KJL99_002589"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HBD4437273.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(5878..8016)
                     /gene="nrdD"
                     /locus_tag="KJL99_002590"
     CDS             complement(5878..8016)
                     /gene="nrdD"
                     /locus_tag="KJL99_002590"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HBD4437274.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVNEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVNVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFSGEHVY
                     DNEKLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(8410..10065)
                     /gene="treC"
                     /locus_tag="KJL99_002591"
     CDS             complement(8410..10065)
                     /gene="treC"
                     /locus_tag="KJL99_002591"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HBD4437275.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(10115..11536)
                     /gene="treB"
                     /locus_tag="KJL99_002592"
     CDS             complement(10115..11536)
                     /gene="treB"
                     /locus_tag="KJL99_002592"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HBD4437276.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(11655..12602)
                     /gene="treR"
                     /locus_tag="KJL99_002593"
     CDS             complement(11655..12602)
                     /gene="treR"
                     /locus_tag="KJL99_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HBD4437277.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMENQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            12787..12840
                     /gene="mgtL"
                     /locus_tag="KJL99_002594"
     CDS             12787..12840
                     /gene="mgtL"
                     /locus_tag="KJL99_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HBD4437278.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            12981..15677
                     /gene="mgtA"
                     /locus_tag="KJL99_002595"
     CDS             12981..15677
                     /gene="mgtA"
                     /locus_tag="KJL99_002595"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HBD4437279.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGDNKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKLTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(15883..16269)
                     /gene="ridA"
                     /locus_tag="KJL99_002596"
     CDS             complement(15883..16269)
                     /gene="ridA"
                     /locus_tag="KJL99_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HBD4437280.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(16342..16803)
                     /gene="pyrI"
                     /locus_tag="KJL99_002597"
     CDS             complement(16342..16803)
                     /gene="pyrI"
                     /locus_tag="KJL99_002597"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HBD4437281.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEEQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(16816..17751)
                     /gene="pyrB"
                     /locus_tag="KJL99_002598"
     CDS             complement(16816..17751)
                     /gene="pyrB"
                     /locus_tag="KJL99_002598"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HBD4437282.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(17755..17889)
                     /gene="pyrL"
                     /locus_tag="KJL99_002599"
     CDS             complement(17755..17889)
                     /gene="pyrL"
                     /locus_tag="KJL99_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HBD4437283.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCLGVRR
                     "
     gene            complement(18170..18565)
                     /locus_tag="KJL99_002600"
     CDS             complement(18170..18565)
                     /locus_tag="KJL99_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBD4437284.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQSEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(18697..19410)
                     /gene="bdcA"
                     /locus_tag="KJL99_002601"
     CDS             complement(18697..19410)
                     /gene="bdcA"
                     /locus_tag="KJL99_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBD4437285.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDVNPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            19481..20074
                     /locus_tag="KJL99_002602"
     CDS             19481..20074
                     /locus_tag="KJL99_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBD4437286.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            20219..20671
                     /gene="tabA"
                     /locus_tag="KJL99_002603"
     CDS             20219..20671
                     /gene="tabA"
                     /locus_tag="KJL99_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HBD4437287.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGAPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            20794..>21532
                     /locus_tag="KJL99_002604"
     CDS             20794..>21532
                     /locus_tag="KJL99_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001558447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4437288.1"
                     /translation="MGKISDLNYSQHITLADNFKQKNEALDTWYVGMNDFARIAGGQN
                     SRSNILSPRAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIEFSSYSKDTDGNEKMKF
                     YMNNPEGERADLSKVKIEITLASASTKGIREGHTVIIFKQSDGSTNRYEGKSFERKDD
                     SSLHLITNKVLACYQREANKKIARLLNNHQKLNNIQELNDSQELNDSQELNNSQKLNN
                     SQELNNSQVSCKGSVDSTITDLLEKALN"
BASE COUNT         5203 a         5326 c         5673 g         5330 t
ORIGIN      
        1 gtggagaggc gcaggagatt acgcgtccgg gaactcacgg ataaagcgtt cgacatcttc
       61 aaccatatgg tcgttgccga caaagaacga acggcgctgg tgcagagttt ccgggatgat
      121 atccagaata cgctctttgc catcgctcgc tttaccgccc gcttgttccg ccaggaatgc
      181 catcgggttg cactcataaa gcaaacgcag tttgccgtcc gggtggctgg cggtgcttgg
      241 gtagagataa ataccgcctt tcagcaggtt acggtggaaa tccgcaacca gtgaaccgat
      301 ataacgcgag gtatacgggc gattggtgga tttatcttct tcctggcaga atttaatgta
      361 cttcttcacc ccgttcggaa acttaatgta gtttccttcg ttgatggagt aggttttgcc
      421 tttctccggg aagcgcatcc gttcctggca caggcagaaa acgcccagcg aaggatcgta
      481 agtaaaggcg tgaacaccgc atccggtggt gtaaaccagc atggtagagg agccgtatac
      541 cacgtaacct gccgcaacct gtttgttacc aggctggagg aaatcttctt ccgttaccgg
      601 cgtgccaaca ggcgtaacgc ggcggtagat ggagaaaatg gtaccgacag agacgttaac
      661 atcgatgttg gacgagccat ccagggggtc catcagcacc acgtattttg cgtgttcaca
      721 gccttcaaag acgacaatct catcttcttc ttcagaggca atccccgcaa cgatatcgcg
      781 tgctttcagt gcggctttca gtttttcatt agcgaacaag tcgagttttt gctgaacctc
      841 gccctgcacg ttctcagcac cgctggcacc caggatatca accagtcctg ctttgttgat
      901 atcgcgatgg ataatcttgg cgcccagttt tattgccgac agcaaagcag tgagctcacc
      961 ggtagcatga gaaaactcgt gctgcttttc gacaataaat tcacctaacg ttttcataaa
     1021 actttccctg caatgtttat ggagtaaagc gaccgcaaca atcttaacaa ataatctcaa
     1081 tgttgcgctc aggtgaatcg cgccagcaaa ttgcggatta tcctgaaatg cgtttctcac
     1141 ttgcccgaca tatgcgtaaa atgagcggca gattaaaaaa ggatagtgac gtatgcgcat
     1201 tcatatttta ggaatttgtg gcacatttat gggcggtctg gcgatgctgg cgcgccagtt
     1261 aggccatgaa gtaacgggtt cggacgccaa tgtgtatccg ccgatgagca ccttacttga
     1321 gaagcaaggc attgagctga ttcagggtta cgatgccagc cagctcgagc cgcagccgga
     1381 tctggtgatt attggcaacg ccatgacccg tggaaatccg tgtgtggaag cggtactgga
     1441 aaaaaacatc ccttatatgt caggtccaca gtggctgcac gattttgtgc tgcgcgaccg
     1501 ctgggtgctg gccgttgccg gtacacacgg caaaaccacc accgcgggaa tggcgacctg
     1561 gattctggaa cagtgtggtt acaaaccggg atttgtgatc ggcggtgtgc cggggaactt
     1621 tgaggtttcg gcgcgtctgg gcgaaagcga cttctttgtt atcgaagcgg atgagtacga
     1681 ctgcgccttc ttcgacaaac gctctaaatt tgtccattac tgcccgcgta cgctgatcct
     1741 caacaacctt gagttcgatc acgccgatat ctttgacgac ctgaaagcga tccagaaaca
     1801 gttccaccat ctggtgcgta tcgttccggg gcagggccgt attatctggc cggaaaatga
     1861 catcaacctg aaacagacca tggcgatggg ctgctggagc gagcaggagc tggtgggtga
     1921 gcagggtcac tggcaggcga aaaagctgac caccgatgct tccgaatggg aagttttgct
     1981 ggatggcgaa aaagtgggcg aagtgaaatg gtcgctggta ggcgaacata atatgcacaa
     2041 tggcctgatg gcgattgcag cggctcgcca tgttggtgta gcgccggcag atgccgctaa
     2101 cgcgctgggt tcgtttatta atgctcgtcg ccgtctggag ttgcgtggtg aagcgaatgg
     2161 cgtcacggta tatgacgatt ttgcccatca cccgacggcg attctggcaa cgctggcggc
     2221 gctgcgtggc aaagttggtg gtacggcgcg cattattgct gtgctggaac cgcgctcgaa
     2281 taccatgaaa atggggatct gcaaagacga tctggcacct tcattaggtc gtgctgatga
     2341 agtcttcctg ctgcaaccgg cgcatattcc gtggcaggtg gcagaagtgg cagaagcctg
     2401 cgttcagcct gcacactgga gtggcgatgt ggatacgctg gcagatatgg tggtgaaaac
     2461 cgctcagcct ggcgaccata ttctggtgat gagcaacggc ggttttggtg ggatccatca
     2521 gaaactgctg gatggtctgg cgaagaaggc ggaagctgcg cagtaattcg gcttcagcct
     2581 gatatagcat tgccggataa ggcgtttacg ccgcatccgg caaaccatac tcacatcaac
     2641 aacgaaaatt acccttcatt ctccgccagt tcgcgcagat actggaaaat ctggcgtgcg
     2701 gatttcggcg gcttattccc ttctttctct ttcttcgcgt tgcggatcag tgtacgcagt
     2761 tgctgacgat cggcatcggg ccacagattc aatacttcag cgatggcgtc atcaccttga
     2821 tcgatcagac gatcgcgcag gttttccagt ttatggaaca gcaccacctg ctggttgtga
     2881 cggtttttca gcttatccag cgcctgacga ataggctcta cgtcgcgctg gcgcagcatc
     2941 ttaccgatga gctgcaactg gcggcggcga ccttccatct taatacgctg ggcgagctca
     3001 atagccgggc gcagatccgc atctaacggg atcttatcca gcgcgttttt ccccagatca
     3061 acaatttccg cgccaaggcg ttttagctcc tcggcatcac gtttaatttc acttttactg
     3121 acccagataa tttcatcgtc ttcgtcttcg atgtcatcac cgggaacgtc gtcgagccag
     3181 tcttcgggct gcttagtcat ctcaggctcc ttaaaaaaag aggctaatgt taccagttaa
     3241 gatgcgcact gaaaaacggt tctctgttag acttcagaga aactctctac attatggcac
     3301 ttgcaatgaa agtaatctct caagttgaag cgcagcgcaa gattctggaa gaagcagttt
     3361 ccactgcgct ggagttggcc tcaggcaaat cggacggtgc ggaagttgcc gtcagcaaga
     3421 ccaccggcat tagcgtaagc acgcgttatg gtgaagtgga gaatgttgaa ttcaatagtg
     3481 atggcgcgct ggggatcact gtttatcacc agaaccgtaa aggtagcgca tcgtctaccg
     3541 atttaagccc gcaggccatt gcccgcaccg tacaggcggc gctggatatt gcccgttata
     3601 cctcgccaga tccctgtgcc ggcgtggcag acaaagagct gctggccttt gacgcaccga
     3661 atctcgactt gttccaccct gcggaagttt ccccggatga agccattgaa ctggcggccc
     3721 gcgcagaaca ggcggcattg caggcggaca aacgcatcac caataccgaa ggtggcagct
     3781 ttaacagcca ctacggtgtc aaagtttttg gcaacagcca cggcatgttg cagggttact
     3841 gctcaacgcg tcattcgctc tccagctgtg taattgccga agaaaatggc gatatggagc
     3901 gtgattacgc ctacaccatt ggccgcgcga tgagcgatct gcaaacgcca gagtgggtcg
     3961 gggccgactg tgctcgccgt actttatcgc gcctgtcacc gcgtaaactc tccaccatga
     4021 aagcgccggt catttttgcc aatgaagtgg caaccgggct ttttggccat ctggtggggg
     4081 cgatagcggg tggctcggtt taccgtaaat ctacctttct gctggattcg ctgggtaaac
     4141 aaattctgcc ggactggctg accattgaag agcatccgca tctgctgaaa gggctggcgt
     4201 cgacgccatt cgacagcgaa ggtgtgcgca ccgagcgtcg cgatattatt aaagatggca
     4261 tcctgactca gtggctgctg accagctact cggcgcggaa actggggctg aaaagcaccg
     4321 gacatgcggg cggtattcac aactggcgga ttgccggaca agggctaagc ttcgaacaaa
     4381 tgctcaaaga gatgggcacc gggctggtgg tgacggagct gatgggccag ggcgtgagtg
     4441 ccattaccgg tgattattcc cgtggtgcag cgggcttctg ggtagagaat ggcgaaattc
     4501 agtatcccgt tagtgaaatc accatcgcag gtaatttaaa agatatgtgg cgcaatattg
     4561 tcaccgtcgg taacgatatt gaaacacgca gtaatataca gtgtggttct gtgctgttgc
     4621 cggagatgaa aatcgccggg cagtaaaaaa ctggcgcgac ctgccgcgct ataaactaaa
     4681 aaattacaca aaatcattcg cactgcatcg agacggaaag tgaatgaggg ccggggagcg
     4741 tactcgcagt acgtgacccg ggcgaatgag cgtagccgtc gatgaggcag tgtgaaggat
     4801 gaagtgtaaa taaaaaagga agtgagcaat gcgtaaaagc ctgttagcta ttcttgcagt
     4861 ctcctcgttg gtattcagtt ctgcgtcgtt tgccgctgat cttgaagaca atatggaaac
     4921 cctcaacgac aatttaaaag tgatcgaaaa agcggataac gcggcgcaag tcaaagacgc
     4981 gttaacgaag atgcgcgccg cagcgctgga tgcgcaaaaa gcaacgccgc cgaagctcga
     5041 agataaatca ccggacagcc cggaaatgaa agatttccgc cacggtttcg acattctggt
     5101 cggtcagatt gacgacgcgc tgaagctggc aaatgaaggt aaaaagaagc gcaggctgct
     5161 gcagagcaac tgaaaacgac ccgcaacgcc tatcaccaga agtatcgtta attcctcatt
     5221 tccctgttgc ctgcactcag gtaacaggga acttatcatc gcaaatgatg caccacctga
     5281 ttgctgctgc cgcgccagat cagggaaggg tcttttaaat cctgcacaaa tttgccgtcg
     5341 accagcacgt taatcaaatc aacaacctgc atttgcgcag cgttgagttc gtcgagttta
     5401 tagcctgtcc acacccagat gtctttaccc ggacactcgg cgcggatgcg ttttaccagt
     5461 ttcagaatat ccggcacgtt ttgcggatgc agcggatcgc cgccggagag ggaaatcccc
     5521 tggcgtttga tacgagtgtc attcagatct ttaatgatct tgtcttccat tgctttggta
     5581 aatggctgac cagaatttac ccgccatgtg cttttgttat agcaaccggg gcattcatga
     5641 acacacccgg agacaaacag ggtgcagcga gtgccggggc cgttgacgat gtcgacagga
     5701 tagtactgat gataattcat tatttcgcct ggcttatcag tccgcgtgta ggctgcatcc
     5761 ggtaatcaat gcctgatgcg acgctgtcgc gtcttatcag gcctacagcc gttgccaaat
     5821 gtaggccgga taaggcgttt acgccgcatc cggcatttgc ttagcgcaga gtgaagatta
     5881 acctatctgc ccattcccca aatgtttaac gcggcgctta acttcttcct gcttaccagc
     5941 gttaaacgga cgtgcatccg ggctacctaa atatccgcac acgcggcgag ttaccgacac
     6001 acgggaggcg tcatggttac cacatttcgg gcaagtgaag cctttgctgg tgcactcgaa
     6061 ctcaccggta aagccacact cgtagcactc atcaatcggt gtattggtgc cgtaatacgg
     6121 tacatgctga tagctgtaat cccagacatc ttccagcgct ttcaggttgt gctgaatgtt
     6181 tggatactcg ccgtagcaaa tgaaaccacc gttcgccagc ggcgggtaag gcgcttcaaa
     6241 gtcgatcttg tcgtacgggt tcaccttctt ctccacatca aggtggaaac tgttggtgta
     6301 gtaaccttta tcggtcacgc ccggcaccac gccaaactca gcagtatcga gacggcagaa
     6361 gcggtcgcac aggttttcac tcggcgtgct gtaaagactg aaaccataac ccgtttcttc
     6421 tttccactga tccactgcct gacgcagacg ttcaacaatg gcgatacctt tcgcgcgaag
     6481 cttctcgttg tcgtaaacat gctcgccgct gaacagcgcg ttaatggttt cgtggatgcc
     6541 gatgtaaccc agcgaaatag acgcacgacc gtttttgaag atttcagaaa catcatcgtc
     6601 agcattcaga cgtacgccac aagcaccttc catatagagg atcggggcga cgcgcgcttt
     6661 cacgccttcg agacgagcga tacgggtcat cagtgcctta cgtgccagca ccagacgttc
     6721 atccagcagc ttccagaagg tggcttcatc gccttttgct tccagagcaa tacgcggcag
     6781 gttcaggctg atcacgccga ggttgttacg accatcgtgg atctgctcgc cgttttcatt
     6841 ttcccacacg ccgaggaagc tgcggcagcc catcggggtt ttaaacgaac cggtgacgtt
     6901 cactacctga tcgtagttca ggatatccgg atacatgcgc ttgcttgcgc actccagcgc
     6961 cagctgtttg atgtcgtagt tcggatcgcc ttttttatgg ttcaggccat cgcgaatcgc
     7021 aaacaccagt ttcgggaaca ccgcagtttt acggttttta ccgaggcctg cgatacggtt
     7081 acgcaggatc gattcctgaa tcaggcgcga ttcccagctg gtgcccagac caaaaccaaa
     7141 ggttacaaac ggcgtctgac cgttggcggt gtgcagggtg tttacttcgt actccagcga
     7201 ctggaaggca tcgtagcact ctttgatggt acgagagtta gcgtagcctt cggcgtccgg
     7261 gatgttccac tcttctgcgg tcttgcgatg tttgttgtag ctggcggtaa caaacggcgc
     7321 cagcacttca tcgatacggt taatggtggt gccgccataa atatggctgg cgacctgagc
     7381 aataatctgc gcagttaccg cggttgccgt agagatcgac ttcggcggtt caatctcggc
     7441 gttccccatt ttaaaaccct gggtcagcat gcctttcagg tcgatcaaca tgcagttgaa
     7501 catcgggaag aacggcgagt aatcgagatc gtgatagtga atatcgccac gctcatgtgc
     7561 ctgcaccacg tcacgcggca gcaggtgctg gcgtgcatag tgtttagcca cgatcccggc
     7621 cagcaggtcg cgctgggttg gaatcacctt gctgtctttg ttggcgtttt cgttgagtaa
     7681 cgaggcgttg gtctgctcga ccagaccacg gatctcctgg ttcaggcgac cgcgtttttc
     7741 acgttcaatg tcgcgatcgt gacggtactc gatgtaagca cgagccagtt gtttgtatgg
     7801 acccgacatc agctggtttt caactgcggt ctggatctcg ttgatatcca cctggttgcg
     7861 cccctgcatc tgctcgttga caaccgcggc aacagtggcg caataatcgg catcatcgac
     7921 ttccgctgct ttagctgcac gcagaatcgc ttctttgatg cgctctgatt taaacggcac
     7981 tttgcagccg tctcgtttca tcacatgcgg tgtcatgatc gctccatatt tttaagaaca
     8041 ggttatccac agaaattggg aaaggcgttt tccggttttt tcgtttcgtt ttccggtgct
     8101 ttccgcaatc gccatccgct tatccacaac gccgcacctt tttcgtgggg agctgtagta
     8161 gcaattatag tcgattaata caacatattg ggttgggacg cattttaaag tctatatata
     8221 gtgctttgca tcaaggatgt tttaactttt tttgatgtag ctcaaagtaa aaagcagagc
     8281 gtacggatga cgggcgctac agcgttatgt aaatttttta atgaatttgc tggttgaaaa
     8341 atcaacaaaa acaacatgct gacagacaaa accccggaat gaccggcatt atcggggctt
     8401 agggaagatt tacttctgta accaccagac agcctcaaaa ggccgtaaat tcatggcaca
     8461 gggttgtggt gaggcttctt cgtagttatg catcacaagc tgccagttgc cgcgcatttg
     8521 ccctggctgc cagggttgga tctcacggct aaggttggca atgaccagca aggtttgccc
     8581 cttccattca cggcgatagc accacaatac agggctgttt ggcagcagat cctggtaatt
     8641 gccccatgtc aggatggctt cttgcttacg cagtgcgatt aacttttggt aggtgtaaaa
     8701 cacagaggag tcatcggcca gcgcggcttc tacgttgatt tgttgatagt tatcgcctag
     8761 gccaatccac ggttcgccag ccgtaaaccc ggcattatcg ccgttgctcc attgcatggg
     8821 cgtgcggctg ttgtcacggg atttactggc gaggattgcc aataactcgt cggcatcacg
     8881 accatcgttg cgcagctcgg caaacatatt gaggctctcc acgtcgcgat agtcagtaat
     8941 gcgcgtgaaa tgcgggttgg tcatgccaat ctcttcgccc tggtagatat acggcgttcc
     9001 ctgcatgcca tgcagcacca tcgccagcat ttttgccgca ggcacgcggt attcaccttc
     9061 atcaccaaag cgagaaacaa tgcgcggctg atcgtggtta caccagaaca aggcattcca
     9121 tgccacgttg tgcattcctt gttgccagtg gcggaacaat gttttcaacg ccacaaagtc
     9181 aggtttagcc agcgtccatt tttcaccacc gggataatcg accttcaggt gatgaaaatt
     9241 aaaggtcatc gacaattcac tgcctgtcag tgccgcgtat cgctggcaat gctcaaggct
     9301 ggtggaggac atttcaccta cggtcattaa cccgcgtggc gtaaacacat cgcggttcat
     9361 ctcgtgcaaa aactcgtgtg ctcgtggccc gtcggtgtag aagcgacgcc cgtcgccatc
     9421 caggtcttca gggaaacgcg ggtctttgga gatcagattc accacatcca ggcgcaaccc
     9481 gtcgacccca cgatcggccc agaattcaca gacttttttc agctctgcgc gtaccgctgg
     9541 attctcccag ttgagatccg cctgttctgg tgcaaagaga tgcaaatagt actgttcgct
     9601 ttccgcatgc cagcgccacg cactaccgcc aaattttgaa cgccagttgt tcggtggcgt
     9661 ttctggttcg ccatcgcgcc agatataaaa ctggcggtaa gggctttctt tgttcagcgc
     9721 ctcgcgaaac caggcatgtt gggtagaggt atggttaaac accatatcga gaatgatacg
     9781 aatcccgcgc gattttgcct gcgtcaccag ttcgtcaaaa tcgtccagcg tgccgtaggt
     9841 gggatcaatc gccgtatagt tcgctacatc gtaaccgtta tcgacctggg gagagacata
     9901 aaagggggtt agccagatgg catcaacgcc cagtttatgc agatagtcca ggcgttggat
     9961 aacgccacgt aaatcgccgg tgccgctacc cgtggtgtcc tgaaaactct ttggataaat
    10021 ctggtagata acgccgtttt gccaccagtg gggaagatta gtcattacgt tattcctgca
    10081 aatgcgaggg ggcgcaattg cgccccgaag aaaattaaac aatgtccagc gtgcccaggc
    10141 ggtatttccg ctgatagata aacgaggtga gtacaatcgg gatgatgatg gcgatagcca
    10201 ttgccagcgc aaatacctgc cagtagctcg gttgaataga gagaattccc ggcaggcccc
    10261 ctacgccgat accattcgcc ataacgccgt tcaggccgca tagcaatcct gccagaccag
    10321 aaccaatcat cgcgcacagc atcgggaagc gatatttcag gttgatgccg tacattgcag
    10381 gctcagtgac cccaagccag gcggagatag cggcaggcac ggagatctcg cgttcattgt
    10441 gcttgcggct ggaaatgata atgcctatca cggcggagcc ctgagcgata ttcgacagcg
    10501 caatcagcgg ccacactggc gtgccaccca tgctttgaat catctgcaaa tcaatagcaa
    10561 gggtggtctg gtgtacgccg gtgatcacca gcggcgcgta caggaagcca aacaatgccg
    10621 caccaatcgg agcaaagctg ccggtcatca ggtgacgtac cgcaaaggca acgccatcgc
    10681 caatcatgcg accaaacgga ccaatcagcg catgggcgag gaacaccgcg aggatcagcg
    10741 aacagacggg caccaccacc agatagaggt aatccggtac gatgcgttta aggcgagttt
    10801 caataacgcc cagcgccagc ccggctaaca gtgccgggat cacctgcgcc tgatagccca
    10861 ctttggcgat gctgaacatg ccaaagtccc acacttccgg cagctgctgc ccgagcagat
    10921 aagcgttcat cagctggggg gaaaccagtg tcacgccaag cacgatacca aggatcggcg
    10981 tgccgcccat ttttttcacc gctgaccagc aaatcccgac cggcaggtag aagaaaatcg
    11041 cttcaccgat caaccacaga aaatcgtaga tcgtttgcag ggaagggtac atttgcgcca
    11101 gcgtctgacc gttgctcatg ggcaaatcgc cgatcacatt gcgaaaaccg aggatcaaac
    11161 cgccgctaat caacgcgggc agcaacggga agaagatctc cgcgaaatga gagatcaact
    11221 gctcatgcca tttcatattt tgccgggcgg ctttttttac ctgctcttta tcaacctgcg
    11281 cctgtccggt tgacgctatt agtgcttgat agtaatcacc cacgttggtg ccaatcacca
    11341 cctgaaattg cccggcattg gtgaaacagc ctttcaccat ggggagttgc tcaatttctt
    11401 tcggtctggc attggccggt tggttgagga caaagcgcag gcgagtaata cagtggctca
    11461 ccgtcgcaat attgccgcgc ccgccgacca gttcaatcaa ccgatcgata tccgtttggt
    11521 ttattttgct catcataaag ccccatggca gatgacattt ttggttggct gcagaatata
    11581 ttgcgcggaa aaatttaaaa acgggaacgt tcccgaaacg cagcgaagat cacaatttat
    11641 cgttcaggaa acgatcagga cagggtggcg gggatgatga tttgttgcgg ttcgctgcgc
    11701 ccggttacct gtgcgatcaa ttggcaggcc gcctggcgtc cagcttcggc gtagccagga
    11761 tctacggtga cgatctccgg atgaaggaat ttcattaacg gcgtattacc tacgctcgcc
    11821 agttgcaagg tgtcgatgcg ttgctcttgc aggtatttac ttgcgccaag agccagcgtg
    11881 tcggttgcgc acagtaacgc ggtagtttca ggcgtaatca cttttgctac gttttcatag
    11941 ccttgcttca tagcaagccc tggcagagcg gcaacgggat gcagtttatg cgctttgcag
    12001 aacgccaggt aggcttcgtg acgtcgcttc ccggtcgtca cgtcactatg cggcacgccg
    12061 agataactga tattgcgatg cccctggtca tagagccgtt gcatcagaat cttgattgcc
    12121 ccttcgtcgt cataacagac cgaagcaaag ccttttgcgt cacgcgccag cagaaccagc
    12181 gatgactgcc agtgggctaa catttcttcg gttatgccgg taaaaccgaa cagcactacg
    12241 ccgtcgatat tacgccgttt cagcaccccc aaatgttcgg caactaattg tggggaaaac
    12301 tgattttcca tcatgattgg atcgtaacct tgttcataga acgctggcag catggtttga
    12361 acggcgagat tttctgacaa cgaatccaga cgggtaacaa tgatggcgac cactttatcg
    12421 ctttgcccac gcatagcgcg cgcagagcgg gaaggggaaa atccatgctg attcatcact
    12481 gcttcaacac gctcgcgcgt gcgctgactc acgccgcttt cgttattcag cacccgggaa
    12541 accgtagatt tccccacgcc gcttaagcgc gcgatgtctt tgatggtcag ccgattttgc
    12601 atcctgttgt cccataacgt gttgtttaat tatttgagcc taactttacc cgtgcattca
    12661 gcaatgggta aagtctggtt tatcgttggt ttagttgcca gcaggtatta tatcgccata
    12721 aatgctacga atattattgg attctcctta ttatttgcgg cgcttttttc acttaccgga
    12781 ggttatatgg aacctgatcc cacgcctctc cctcgacgga gattaaaact tttccggtaa
    12841 gcccgtcttt tcacggcgtt accggatgcg taaggccgtg acgttttaat gtccctgctc
    12901 agctttatta ccttcaggta aggcttcgcc acgcctgaag acatttctgt actgtttcag
    12961 acagtgcgga gggactcctt atgtttaaag aaatttttac ccggttcatt cgccatttac
    13021 cttcccgtct ggttcatcgc gatccattgc ctggcgcgca gcagacagtg aatacggcgg
    13081 tcccgccgtc cttaagtgcg cattgcctga aaatggcggt gatgcccgaa gaagaattgt
    13141 ggaaaacgtt cgacacccat ccggaaggat taaatcaggc ggaagtggaa tctgcccgcg
    13201 aacaacatgg tgataataaa ttacccgccc aacaaccgtc gccgtggtgg gtacatttat
    13261 gggtctgcta tcgcaacccc tttaatattt tgctcaccat tcttggtgct atttcttacg
    13321 ccacggaaga tttatttgcc gcaggtgtta tcgcgctaat ggtcgctatt tctacgttgc
    13381 tgaactttat tcaggaagct cgttccacca aagcggcaga tgccctgaaa gcgatggtca
    13441 gtaataccgc gacggtactg cgagtaatta acgacaaagg cgaaaatggc tggctggaga
    13501 tcccgataga ccagctggtg cccggcgata ttataaaact ggcggcgggg gatatgatcc
    13561 cggcagattt acgtatcttg caggcgcggg atctgttcgt cgctcaggcg tcgttaaccg
    13621 gtgagtctct gcccgtggaa aaagccgcta ctactcgcca gccggagcac agcaatccgc
    13681 tggagtgcga caccctgtgt tttatgggca ccaccgtggt gagcggcacg gcacaagcaa
    13741 tggtgattgc tacaggagcc aacacctggt ttggtcaact ggcggggcgt gttagtgagc
    13801 aggaaagcga gccgaatgcc tttcagcaag ggatcagccg cgttagtatg ctgctgattc
    13861 gctttatgct ggtgatggct ccggtggtgt tgcttatcaa cggttacacc aaaggcgact
    13921 ggtgggaagc ggcactgttt gcgctttcgg tcgcggtagg cttaacgccg gaaatgttgc
    13981 cgatgattgt tacctcgacg ctggcgcgcg gcgcagtaaa gctgtcgaaa cagaaagtga
    14041 tcgtcaaaca tctggatgcc attcagaact ttggcgcaat ggatattctg tgcactgata
    14101 aaaccggcac cctgacgcag gacaaaattg tgctggagaa tcataccgat atctccggta
    14161 aaaccagcga acgcgtgctg catagcgcgt ggttgaacag tcattaccag accggactta
    14221 aaaatctgct tgataccgcg gtgctcgaag gtacggatga agagtcagcg cgttcgctgg
    14281 ccagccgttg gcaaaaaatt gatgagattc cgtttgattt cgagcgtcgt cggatgtcgg
    14341 tggtagtggc agaaaatacc gaacaccatc agctggtttg caaaggtgca ttgcaggaaa
    14401 tcctcaatgt gtgttcgcag gtgcgtcaca atggcgagat tgtaccgctc gatgacacca
    14461 tgctgcgtaa gattaagcgg gttactgata cgctgaatcg tcaggggctg cgcgtggttg
    14521 cggtggcgac gaaatacctg ccagcgcgtg aaggagatta ccagcgggcg gatgaatccg
    14581 acctgatcct cgaaggatat attgctttcc ttgatccgcc aaaagagaca accgctccgg
    14641 cactgaaggc attaaaagcg agtggaatta ccgtaaaaat ccttactggc gacagtgagt
    14701 tagttgcagc gaaagtgtgc catgaagtgg ggctggatgc gggagaggtg gtcattggta
    14761 gtgatattga aacgctatct gacgatgaac tggcaaatct cgcacagcgc accacgctgt
    14821 ttgcccgcct gacgccgatg cataaagaac gcattgtgac tttactgaag cgcgaagggc
    14881 atgtggttgg ctttatgggc gatggtatta atgatgcgcc cgccttacgc gctgcggata
    14941 tcggcatttc tgtggacggc gcggtagata ttgcccgtga agcggctgat atcatcctgc
    15001 tggaaaaaag cctgatggtg ctggaagagg gggttattga gggacgtcgc actttcgcca
    15061 acatgctgaa atacatcaaa ttgacggcga gttctaactt cggtaatgtg ttcagcgtgc
    15121 tggtggcgag tgctttcttg cccttcctgc cgatgttgcc gttacacttg cttattcaga
    15181 acctgctgta cgatgtgtca caggtggcga tcccgtttga taacgtcgac gacgagcaaa
    15241 ttcaaaagcc gcagcgttgg aatccggcgg atctggggcg ctttatggtc ttcttcggac
    15301 cgatcagctc gattttcgat attctgacgt tttgcttgat gtggtgggta ttccatgcca
    15361 acacgccgga aacgcaaacg ctgttccagt cgggctggtt tgtggtggga ttactgtcgc
    15421 aaacgctgat tgtgcatatg atccgtaccc gccgtgtgcc gtttattcag agctgtgcat
    15481 cgtggccgtt aatgatcatg accgtgatcg tgatgattgt cgggatcgca ttgccgtttt
    15541 caccgctggc cagttatctg cagctgcagg cgctgccgtt aagctatttc ccgtggctgg
    15601 ttgcgattct ggcagggtat atgacgttaa cccagttggt gaaagggttc tatagccgtc
    15661 gttacggctg gcagtaaaga ataaactggg catggtggcc cggattcaga ttgcagacaa
    15721 agtgagcttt gttcatgccg gatgcggcgt aaacgcccaa tccggcctac aaattcaata
    15781 aattgcagga atcacgtagg cctgataagc gtagcgcatc gggcgatttt gcttttgtca
    15841 tcagacttaa tccgggcatg atagcccgga tttccatcaa gattagcgac gaacagcgat
    15901 tgcttcgatc tcaatcttca cgtctttcgg cagacgggca acttcaacgc aagaacgtgc
    15961 cgggaaagtg gcgttgtgtt cggtgaagaa ggcttcgtaa gtggcgttta cggttgcgaa
    16021 gtcgttcaga tctttcacga acacagtggt tttaacgatg tcgcccactt tcaggccagc
    16081 ggcttcgacg attgctttta cattatccag cgactgacgt gcctgtgcag cgacgtctgc
    16141 cggtacttca ccagttttcg gatttaccgg gatctgaccg gaggtgatga tcatattgcc
    16201 cagatcaacg ccctgtacgt aaggaccgat agctgccggt gcattttccg tcgcgatagt
    16261 tttgctcatg atttctcctt tattacagcg gtaaaaagtc tggctcatta tagggagcca
    16321 gacttttatt accaaccgca attaattggc cagcaccaca ttatgggaaa actctttttc
    16381 acagtatttg catttaagcg cgatgtcatt ggcgcgtttt cgcacggcaa agctggatga
    16441 aaccggttcg gcatggctga tacagttgct gttcgggcag accagcacat tgtcgatgcg
    16501 ctctggcaga cttgggcgcg atttacccac cacttcatag ttgtcgatac ggttaaccgt
    16561 ggcttgcggt gcatacaatg ccagttggtc tacttgctct tcactcaaaa aggtattttc
    16621 gattttgatc agatctttgc ggcccatctc gccagaaggc aaattcagac caatggtgat
    16681 gcgctgatct gtttcggtca acttgaacag actcaacagc ttaaaaccga tctgggcggg
    16741 gatatggtca attaccgtgc cgcgtttaat agcttcaacc tgcaatttat tatcgtgtgt
    16801 catctctatt tccccttaca gtaccagatc gcgattcaga accagtgcca gtaacgcctg
    16861 gcgagcgaaa atcccgttgc ctgcttgctg gaagtaccag gcgtgaggcg ttttatccac
    16921 atccgtcgca atctcatcaa cacgcggcag cggatgcagc actttcatat tggctttggc
    16981 gttgtggaga tcgctggcgc gaagaacaaa ctgcgctttc acgttggcgt actcggacgg
    17041 gtccagacgc tctttttgca cgcgggtcat gtacaggatg tctacttccg ccatcacttc
    17101 ttcaatagag ctgtgcagac tccaggcgat ccctttttca tcgagcatat ccagaatgta
    17161 ttgcggcatt gccagcgcat ccggcgcgat aaagtaaaaa cggttgccgt cgaacttcgc
    17221 tagcgcctgg gtcagggagt gaacggtgcg gccatatttc aggtcaccaa ccattgcgac
    17281 gtggagattg tccagacgcc cctgggtttc ctgaatggtg aataagtcca gcaaggtttg
    17341 cgtcggatgt tggttggagc catcaccggc attcagtacc ggtacattgc cagaaaattc
    17401 ggtggctagg cgtgccgcac cttcctgcgg atgacgcatc actatcgcat cgacgtaagt
    17461 gctgataacc gaaatggtat cggccagcgt ttcgcctttt ttacccagtg atgtattggc
    17521 gctgtcggag aagcccacca cgctggcacc caggcggtgc atggaagttt cgaaagagag
    17581 gcgggtacgg gtagaggctt cgaagaaaca gctggcaatg actttgtgct tcaacagctc
    17641 tggttgcggg tttgctttca gtttcgccgc tgtcgccagc accagattaa ggtcatcgcg
    17701 actaaggtcg tttatggaaa tgatatgttt ctgatatagc ggattagcca tcttttatct
    17761 cctgacacct aggcaaaaaa aagcccctcg attgaggggc tgggaatggg tgatcaacgg
    17821 gaagaaaaac ggcaggccag cgtctttttt cagacgcggt aagacaaaat gtcgaacaca
    17881 ctgaaccata catcctcccg gcaaattgtc cggcattata ctcatagcca gaagcggtgc
    17941 aagcatttga tgcaatattt tgtcagcgca aacggtttat ttgaattaaa agtcaaaata
    18001 tatgcatttt tattttctat gattctggag gggaagtgtc gttcggtatc aataattgca
    18061 gacatttcct gcttttttct ttttcatttt cacgcaatca gaaatgcatt ttattccatt
    18121 catccgactt atagggcgga gggtgaaaac gaacggctaa cactattgct tatagctcag
    18181 ggatacgcgc tatcacttta atttcaaaat caaagcctgc cagccaggta acacccaccg
    18241 ccgtccagtt tggataaggt ggggcgctaa atatttcatt tttcaccgtc atgatgtctt
    18301 cagattgttt ttccggatcg gtatgaaagc tcgtaacatc aatgatatcg tcaaaagtgc
    18361 atcccgcagc tgccagggtc gcatgcaaat tatcaaaggc cagtctgact tgttgctgaa
    18421 aatcgggttc tggtgttccg tcctctcgac ttcctacttg cccggaaaca aacagcaaat
    18481 cgccggaacg aatagccgca gaataacgat gctcagcata cagtgaatgt cggccagcag
    18541 ggaaaacagc ggttctttct accatttggt tatcctcaag atttacgaca tgaacagaag
    18601 atttatattt atcgggagcc gcttttaggg ggacgacgtt agtaaacaaa acccagacat
    18661 catggataat ggctgggctt aattgagcgt agtcggttat gcgccaaacg cgccatcaat
    18721 ggtatgcatc gcgccggtaa caaaactggc ttctggccct gctaaccatg cgaccatacc
    18781 agcgacctct tccggttgcc catgtctttt gatggccata aaactatgca acatatcgcg
    18841 cattggcccg ttggcgggat taacgtcggt atcaattggt cctggctgga cgacgttgat
    18901 ggtgatccct cgcggtccaa aatcacgggc cagcccgcgc gccatgcctt gcagggcaga
    18961 tttactggcg gcataagcag ccatgcctgc aacaggcata cgatcgccat tcacggagcc
    19021 gatgattaag atgcgcccgc cttcgggcat ctgccgggcg gcttcaacag aggcatgata
    19081 aggagcatga atattgattt tgaaaaggcg atcaatatcg tcggcattta attccagggc
    19141 atcgccaaag acgccaatac ctgcatttac caccaggata tccaatgcgc cgctcttacg
    19201 aacgacatca ataacagcgt ctctgtcagc actatctgtg aatactgctg tcgctccagt
    19261 ctcttgtgcc agacgttcag cggcatcttt cgaccccgca taggtgaatc gtacattggc
    19321 cccatcggtg acgaaacgac gtacgatagc ggcaccgatt ccacgactgc caccgaggat
    19381 gagaactgtc ttaccggtaa aagcgcccat aaggactcct tgatttatta tgtaacatgc
    19441 attacaaaac tgttttaact ttctgtcaac aggttttgta atggtcacta aaaaacaatc
    19501 tcgcgttcca ggtcgtccca gacgtttcgc tcctgagcag gcagtctctg cggcaaaagt
    19561 gctttttcac caaaaaggtt tcgatgcagt aagtgtctct gaagttactg attatcttgg
    19621 tattaacccc ccgagtctct acgcggcttt tggcagtaag gctgggttat ttagccgtgt
    19681 actcaatgaa tatgtcggta cggaagctat tccgcttgcc gatattcttc gtgatgatcg
    19741 tccggtaggc gagtgcctgg ttgaggtatt aaaagaagcg gcgcgcagat atagccaaaa
    19801 cggcggctgc gctggctgta tggttcttga aggtattcat agtcatgatc cacaagcgcg
    19861 tgatattgcc attcaatatt atcacgccgc agaaacgacc atttatgact atatcgccag
    19921 gcggcatcca caaagcgcac aatgtgtgac tgattttatg agtaccgtga tgtcagggct
    19981 ttctgcgaag gcacgggagg ggcactccat cgaacaactc tgtgcaacag ctgccctggc
    20041 aggggaagcg ataaaaactc ttctcaagga gtgatgctgg ccttgatccg aaaggcggga
    20101 acgcgcctgc cgataagttg tgataagaca ataattcacg cgttaaggct ggcggaattg
    20161 atcatctttt cgtataacga tagaaatgaa acattgtttt aattaaggag tggaaaagat
    20221 gatcatcgga aatattcata atcttcagcc gtggctaccc caggagttac gccaggcgat
    20281 tgagcatatc aaagcacacg ttacggcaga aacgccaaag ggcaagcatg atatcgaagg
    20341 caatcgtctg ttttatctta tctcggaaga tatgaccgag ccgtacgaag ctcgccgtgc
    20401 ggagtaccat gcccgctatc ttgacattca gattgtgtta aaaggtcagg aaggcatgac
    20461 cttcagcacg caacctgcag gcgcgccgga taccgactgg ttagctgata aagacatcgc
    20521 atttttgccg gaaggcgttg atgagaaaac agttatccta aatgaaggtg attttgttgt
    20581 gttttatccg ggggaagtgc ataaaccgct gtgcgcagtg ggcgcgccag cccaggttcg
    20641 caaagcagta gtgaagatgc tgatggcgtg atgacttttc gccgtaaata actccaggtt
    20701 tacggcgagt ttgtgaaaag agcgtttttt gatatttttt tgtgagtaaa atttgtaatg
    20761 cttagacgtt cttattaact caaggagttc gtcatgggca aaatatcaga tttgaattat
    20821 tctcaacaca ttacattagc cgacaatttt aaacaaaaaa atgaagcctt agatacctgg
    20881 tatgtgggaa tgaatgattt tgcccgaatt gccggggggc agaatagcag aagcaatatt
    20941 ctttctccta gagcattttt ggagtttttg gcaaagatat ttaccctggg ttatgtggat
    21001 tttagcaaac gctccaacga agcgggtaga aatatgatgg ctcatattga gttctcatct
    21061 tattctaaag atactgatgg caatgaaaaa atgaagtttt acatgaataa tcctgaaggg
    21121 gagcgagcgg atttatctaa ggtgaagata gaaattacac ttgcctcagc ttctactaaa
    21181 gggattcgtg aaggacatac agtcattata tttaaacaat ctgatggttc gactaatcgt
    21241 tatgaaggga agtcttttga aagaaaagat gatagttcat tacacctgat tactaacaag
    21301 gttctggcgt gttaccaacg cgaagctaac aagaaaatag cgcgtctatt aaataatcat
    21361 cagaagttaa ataatataca ggagttaaat gattcgcagg agttaaatga ttcgcaggag
    21421 ttaaataatt cacagaagtt aaataattcg caggagttaa ataactcgca ggtgagttgt
    21481 aaaggttcag ttgattctac gattacggat ttattagaaa aagcattgaa ta
//
LOCUS       DAEBXN010000068        21278 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.068, whole genome shotgun sequence.
ACCESSION   DAEBXN010000068 DAEBXN010000000
VERSION     DAEBXN010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21278
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.068"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(<1..446)
                     /locus_tag="KJL99_002605"
     CDS             complement(<1..446)
                     /locus_tag="KJL99_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HBD4437289.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRIPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLV"
     gene            complement(501..1352)
                     /gene="manZ"
                     /locus_tag="KJL99_002606"
     CDS             complement(501..1352)
                     /gene="manZ"
                     /locus_tag="KJL99_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HBD4437290.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(1365..2165)
                     /gene="manY"
                     /locus_tag="KJL99_002607"
     CDS             complement(1365..2165)
                     /gene="manY"
                     /locus_tag="KJL99_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HBD4437291.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(2228..3199)
                     /gene="manX"
                     /locus_tag="KJL99_002608"
     CDS             complement(2228..3199)
                     /gene="manX"
                     /locus_tag="KJL99_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HBD4437292.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            3662..5212
                     /gene="yoaE"
                     /locus_tag="KJL99_002609"
     CDS             3662..5212
                     /gene="yoaE"
                     /locus_tag="KJL99_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HBD4437293.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGNGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIFEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(5216..6814)
                     /locus_tag="KJL99_002610"
     CDS             complement(5216..6814)
                     /locus_tag="KJL99_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBD4437294.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSKVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVIEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQSFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTH
                     YVMAETIRQHHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMSLR"
     gene            complement(6945..8309)
                     /gene="sdaA"
                     /locus_tag="KJL99_002611"
     CDS             complement(6945..8309)
                     /gene="sdaA"
                     /locus_tag="KJL99_002611"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HBD4437295.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(8493..9071)
                     /locus_tag="KJL99_002612"
     CDS             complement(8493..9071)
                     /locus_tag="KJL99_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HBD4437296.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINREPLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVTP"
     gene            complement(9075..10436)
                     /gene="pabB"
                     /locus_tag="KJL99_002613"
     CDS             complement(9075..10436)
                     /gene="pabB"
                     /locus_tag="KJL99_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HBD4437297.1"
                     /translation="MKTLSPTVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTHEQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            10510..10689
                     /locus_tag="KJL99_002614"
     CDS             10510..10689
                     /locus_tag="KJL99_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HBD4437298.1"
                     /translation="MFAGLPSLIHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGELIVARFEDEDE"
     gene            complement(10809..11168)
                     /locus_tag="KJL99_002615"
     CDS             complement(10809..11168)
                     /locus_tag="KJL99_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HBD4437299.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIK
                     NPEYFSTYMQEELKALV"
     gene            complement(11530..11874)
                     /locus_tag="KJL99_002616"
     CDS             complement(11530..11874)
                     /locus_tag="KJL99_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBD4437300.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            12006..13916
                     /locus_tag="KJL99_002617"
     CDS             12006..13916
                     /locus_tag="KJL99_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HBD4437301.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQIVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDYRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            13974..14669
                     /gene="tsaB"
                     /locus_tag="KJL99_002618"
     CDS             13974..14669
                     /gene="tsaB"
                     /locus_tag="KJL99_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HBD4437302.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            14709..15290
                     /gene="yeaY"
                     /locus_tag="KJL99_002619"
     CDS             14709..15290
                     /gene="yeaY"
                     /locus_tag="KJL99_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HBD4437303.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSTTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            15496..17181
                     /gene="fadD"
                     /locus_tag="KJL99_002620"
     CDS             15496..17181
                     /gene="fadD"
                     /locus_tag="KJL99_002620"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HBD4437304.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGIIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            17263..18378
                     /gene="rnd"
                     /locus_tag="KJL99_002621"
     CDS             17263..18378
                     /gene="rnd"
                     /locus_tag="KJL99_002621"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HBD4437305.1"
                     /translation="MITTDDALASLCEAVRVFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(18432..19397)
                     /gene="yeaX"
                     /locus_tag="KJL99_002622"
     CDS             complement(18432..19397)
                     /gene="yeaX"
                     /locus_tag="KJL99_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HBD4437306.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHIL
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDIDWQLHYCSRNPESCAFRAELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(19453..20070)
                     /gene="yeaW"
                     /locus_tag="KJL99_002623"
                     /pseudo
     CDS             complement(19453..20070)
                     /gene="yeaW"
                     /locus_tag="KJL99_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
     gene            complement(20412..20978)
                     /locus_tag="KJL99_002624"
     CDS             complement(20412..20978)
                     /locus_tag="KJL99_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBD4437307.1"
                     /translation="MPPEIMRFKERLEEAMNGESSRAFAAKCGLSDGVIRNYLSGKTY
                     PSLDRLAQIAYATGRPIEWFIQSQSMPMVEHKNNQENKNSQPDRSTLKTKLIAIVELL
                     EDKELHSAIELFRKKGFEALMPEIFSSDDSIQADLQGISQQTLQTAKMLESLPTDTRK
                     KILSKYGIYEQEGLVAPSQEPQDVKKAV"
     gene            21190..>21278
                     /locus_tag="KJL99_002625"
     CDS             21190..>21278
                     /locus_tag="KJL99_002625"
                     /inference="COORDINATES: protein motif:HMM:NF025073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBD4437308.1"
                     /translation="MSAKNQDWHAEQIKAAVRMKGKTLSQLSR"
BASE COUNT         5266 a         5273 c         5466 g         5273 t
ORIGIN      
        1 acgagaagct tataaatttt ttccagatcg tcgatatttc gaacgcggat taacagccga
       61 cgctgttcta attgcatcac caacacgcca tcttccgaca agttcatcgc tttgattcgg
      121 ctatattcaa tccagacatt ggcgaagaaa aaaccttttt gtttaaagat gatcttcgga
      181 atgcggatcc agaatatata aaaacccatc agagccagtg cacttaataa ccatgttgtt
      241 attagcgcgc catgattcgt gacgttgtta tagataagaa tgacaatcaa tccgacgaag
      301 ataacgctat cgatgcgacc acgccggagc aaaggaattg ccagcagggt ggggccgtta
      361 cggcggggca tgatgaactg atcgtagatc gcgaaggcca ggagtgcggc gatgaaaaga
      421 atcagtacca ggtccgtgat tgtcattaat cctccagata aaaaaacggg gccaaaaggc
      481 cccggtagtg tacgacagtc ttacagtccc agcaggccgc aagcgtaacc agcgataccg
      541 atgacgaaga agccaacgat gatccacagc gggttaactt ttttgcgcag cagccacata
      601 caagcaaatg tcagcagcag tggtacaagg cctggcatta actggtcaag aatagtttgc
      661 acggtggtga cacgggtctg tccatcctgc ccggtgatgg tcgaaaccac cagcgggatg
      721 ttgacatgtg tccacttgtt aaccaatgcc cccatgacaa acaggccgag gatagacgcc
      781 ccttccgtca gtttttgcag gaagccacca cccatatctt taacgatatc gatacctttg
      841 gagtaaccat acgctacgcc gtagtaacgg gttgccagac gcaccaggtt aaacaggatg
      901 aagaacagca gcggacctaa caggctgccg ctcatcgcga taccggcacc cagtgctgca
      961 aataccggac gtacggttcc ccagaagatc gggtcgccta caccagccag cggccccatc
     1021 aaaccgactt tgataccgtt gatagcaccg tcatcgatct ctgcgccatt agcacgctgt
     1081 tcttccagcg ccagggttac gccgagaatc ggtgcagcca cgaacggctg ggtgttaaag
     1141 aactccaggt gacggcgaat agcttgttta cgagcttcgt tgttctcagg gtagaggcga
     1201 cgaattgccg gtaccataga gaagcagaaa cccagtgcct gcatacgttc gaagttccat
     1261 gaaccctgga agaggttaga acgcaggaat acgccacgaa tatcactttg agtgagtttt
     1321 ttctcggtgg tagtttgagt tgtatcaacc atttcgctca cctgttagtc cagttcgtta
     1381 tcgagatcgt tgttaccagc tgcctgagca ggcgcaccgg ctacgcggtt gtatttcggg
     1441 ctaagttgga tgtagagcac tgccataaca gtaccaatca cacccagagc aaccaggtta
     1501 aagttggtga atgctgcggt tacgaagccg aggtagaaga acggcatcag gtagccagca
     1561 cgcatcatgt tgataaccat cgcataacca accaccacga tcatgccccc ggcgatattc
     1621 agaccattgg tcaccacttc cggaatcgca ttcagcatgt tctgtacttc gctggtacca
     1681 acagacagcg caacgatgac ggccggaata gccacacgca ttgcttgcag gaacagagaa
     1741 gaaacgtgga tccaggaaat cgctgtcagg ttgccgttat cagcagcctt atccgcagcg
     1801 tgctggaaag caacggtaat agtacgaacg atgatggtca gtacctggcc cgcagcggcc
     1861 agagggattg ccagtgcgat acctgcacca atgctctgat gacctgcgat aaccagaatg
     1921 gtagaaatga tagaagccag agcggcgtca ggcgcaactg cagcaccgat gttcatccag
     1981 cccagcgcga tcatttccag cgtaccaccg ataataatac cggttttcat atccccaaga
     2041 acgataccca ccagggtaca cgcgattagc ggacggtgaa actgaaattc atcgaggatt
     2101 gatcccatac ctgcgataca ggctacgata aataccagca caatttgaag agtggtaatc
     2161 tccattgtac ttctcctgtt tacgtaagac ttaagtgtga aaactgaatg ataatcaaca
     2221 caatacgtta cttatcgatt ttgctgatca gatccatcat tttcagtttc ggatcggtgg
     2281 aaaccttacg gacttccagc tcaataccgc gtgcattcag ttttttgaac gcctcgatat
     2341 ctttttcatc aaccgaaacc gcgttattca cctgggtttt accctgacgg aatgccatac
     2401 caccgacgtt aacagaggtg attttcacgc cgccttcaac gagacgctct acatctgttg
     2461 ggttggtaaa taacagcatt acgcgttcgc cagcatattt cgggttgttg tagacgcgaa
     2521 tcattttggc aacatcaact acgtgtgctg ttacgcccgg aggtgcaacc tgggtgagca
     2581 gtgtcttacg aacggtatcc gcagccactt catcactaac aacaataata cgggagacat
     2641 tggtttcttt ggtccagcgg gtggcgacct gaccgtgaat cagacggtcg tcgatacgcg
     2701 caaggccaat aaccatgtag tcgtttggcc ccattggttt tgccggagtt ggagccgctt
     2761 ttggtgctgc ggcagcgggt gctggcgcgg ctttttcaac cggtttggct ttcagtgctt
     2821 tcacgccttc acggcctgtt tctactgcca gcgcaaccag ttcatcaaag ctcgggtcat
     2881 catcacgggc cattaacgtt tccacgagca ttggaatgtt aacgcctgca atgacttcat
     2941 aatgctcttt gtcgacgaca atgcggctgg cagcattgaa cgggctgcct ccccatgtat
     3001 caacgagaaa cagcacgcct ttagtggtgt cgagttttgc caactgagcg ttgtattttt
     3061 caatcagcgt ttcggcattt tcacctggaa cgaaatcgat ccagccgacg ttttcctgct
     3121 cgcctaacag catttctgcc gttttaagca actgctctgc agcccaacca tgtgtgccta
     3181 taacaatagc aatggtcact tgctacctcc tttattatcg ttaacacctc aacgtgccag
     3241 atgtattttt gaatcgtcgt ccacaatcga atcgattcag ataagggcta caaccaaaaa
     3301 cccctgcgtt tcgcgattta ttttagatat cgaaaaaatt attttatgtg atgaagatcc
     3361 gtaatttaac tttcgattat cagaaatttc gaaaggtaaa atatccttgt cacattcgtt
     3421 tgcaaaggaa ggtaaatctt tgccaaatca gaggcgtctc tgatatgttt aactcccgtt
     3481 taacaaccat ggagtatagg gcagtagccc gcagtatgga tcgtcaccga cgtcatttca
     3541 gcatcaggcc ttttaacgcc tgcctttctg gcactctatg ccgcaccttt cgtttgcatt
     3601 ttgtcgttac gcctgcatta tttctggcgt cgaatagcta ttccttaagc aggagcttgt
     3661 catggaattc ttaatggacc cctcaatttg ggcggggcta ctcacgcttg ttgttctcga
     3721 aattgtgctg ggtatcgata acctggtctt catcgccatt cttgctgaca aactgccgcc
     3781 aaaacaacgc gataaagcgc gtttgctggg gttatcactg gcgctgatta tgcgtctggg
     3841 gctgctgtcg ctgatttcat ggatggtcac gctgaccaaa ccgctattta ccgtcatgga
     3901 tttctccttc tccggacgcg atctgattat gttgttcggg gggatattct tgctgttcaa
     3961 agcaacaacc gaactgcatg aacggctgga aaaccgcgat catgattccg gccacggtaa
     4021 aggctacgcc agtttctggg tggtcgtcac acagattgtc atccttgacg ccgtcttctc
     4081 gttggatgcg gtaattactg cagtagggat ggttaaccat ctgccggtga tgatggcggc
     4141 ggtagtgatt gcgatggcgg ttatgttgct ggcatcgaaa ccgctgacgc gattcgttaa
     4201 ccagcacccc acggtggtgg tgctctgtct gagcttcctg ttaatgattg gtctgagtct
     4261 ggtggcagaa ggtttcggtt tccacattcc gaaaggttac ctgtatgccg cgattggctt
     4321 ctcgatcatt atcgaagtgt ttaaccagat tgcgcgtcgc aactttattc gtcaccagtc
     4381 gactttgccg ctgcgagcgc gtactgccga tgccatcctg cgtttgatgg gcgggaaacg
     4441 tcaggccaat gttcagcacg atgccgataa cccgatgccg atcccggaag gtgcatttgc
     4501 cgaagaagaa cggtacatga ttaacggcgt actgacgctg gcgtcgcgtt ctctgcgcgg
     4561 gatcatgacg ccgcgcggtg aaataagctg ggttgacgct aatctcgggg tcgatgaaat
     4621 ccgcgagcaa ctgctctctt caccgcacag tctgttcccg gtatgtcgcg gtgaactgga
     4681 tgaaatcatc ggtatcgtac gtgctaaaga actgctggtg gcgctggaag agggcgttga
     4741 tgtggcggcg attgcttcgg cgtctccggc gattatcgtc ccggaaaccc tcgatccgat
     4801 caacctgctg ggcgtgctgc gtcgcgctcg cgggagcttt gttatcgtga ccaacgagtt
     4861 tggtgtggta caaggcttgg tcacgccgct ggatgtgctg gaagccattg cgggtgaatt
     4921 cccggacgct gacgaaacgc cggaaatcat taccgatggt aacggctggc tggtaaaagg
     4981 cggtacagat ttgcatgcct tgcagcaggc gcttgatgtt gagcaccttg ccgatgacga
     5041 tgatatcgcg acggtcgcgg gcctcgtgat ctcggcaaat ggtcacattc cccgtgtggg
     5101 cgatgtgatt gatgtagggc cactgcatat caccatcttt gaagccaatg attatcgtgt
     5161 tgatctggtt cgcattgtta aagagcaacc ggcgcacgat gaagatgagt aagcgttaac
     5221 gtaacgacat aatgggcgtg atatgtccat tatgccgggc gggcggcggt tggctgcccg
     5281 ccagccattt gggaaaatca cgtagcggca tcggctgtgc gtacaaatac ccttgcaaaa
     5341 tatgcacgcc atgacggcgc aaatacttcg cctgttcttg cgtttccacg ccctccgcca
     5401 ccagttcaat attcagtctt tgccccagcg cgatgatgat atcggtcacc gtcgagttaa
     5461 ccgcgtcaga acctatagct gcggtaaatg acttatcaat tttcagcacg tcaggacgta
     5521 atgtttcaag ccaggaaaac gagctgttgc cggtgccgaa gtcatcaatc gccagtttga
     5581 cgtttttacg atgcagctcg cgggcaatcc gataatcaac atccagtaag gcatcgcgtt
     5641 cggtgatttc gaggatcagt tgctgaatcg ggtgagcgct aaaccagtac tgattgagat
     5701 cttttatcag cacaccacgg cgaaaatggc tgggtgcgac gttaatgcca acatgaaact
     5761 gactactcat cgggaaaaca tggtgctgac gaatggtttc tgccatcaca tagtgggtca
     5821 gtggcacaat taaatgatgt tcttccgcga taggaataaa cacatccggt gaaatccagc
     5881 cctgacgcgg attattccag cgcagcaaaa tctctacacc aatacattgc tggctgcgcg
     5941 cattaagcaa aggctgacag aacaattcga actcatgttg cgccaggccc agattaattt
     6001 cgcgggaaaa gctcatccgg taagcggtcg ccagccaggc gatatagccc accagtaaac
     6061 ttagcagcac cgccagcggt aattgtgttg gaagatagtg ccatgccagc gccgtagccc
     6121 ccggaccatt tacgttaatg gtaaaaggaa agctttgcga agaaacctga tagttttcat
     6181 tgtcttcagg ttgcggaagg gaatctacca gcccattacc atagagcaaa tgccgtttgt
     6241 ccaccgtcag gctggcgcta ctgatttgcg gcagttgtgg ctcaaggagc attgccgtca
     6301 gtaagtcgat gttaatcatc tctatgaccc cagcattgct actgcccgct gcaggcgtcc
     6361 attgaatcaa aaccggactg cctttaatca gggcacggtc gatcgtcagg cgtaaaagtg
     6421 gttgcggtgc aggaagttca gccagaatgt ccacgacggg cacattgcga taaccaaaaa
     6481 tgctggagca ataaagtgtg ccgtcttgca ccaggccaat ggatcgcaca gtttggagtt
     6541 ttgccgcctg tttacgtaat ggcaaatggg cgacagagca gggcagacca atcagcggaa
     6601 gcaagacttt gctacctgcc tgtaggggaa gcagtacttt atctaattcc tctacagcgt
     6661 gattggcgaa ttgtactacc cgttgttgat ttaagttacg ctgtgaaata aatcgcacgc
     6721 tcagggtcga agcgagcgta acgagtgcgc aaatcgtaca aacaatcatt cgattacggc
     6781 gataggtttt aatgatccgt tgtgcttttt gcatgcgggc caccctgtaa gcgtatggcg
     6841 acaaacgcct gacgcaacag tggaagtgta gtggggaata ttacagcaga cgagaaagcg
     6901 ggtataaatt cgcccatccg ttgcagatgg gcgagtaaga agtattagtc acactggact
     6961 ttgattgcca gaccaccgcg tgaggtttcg cggtatttgg cgttcatgtc cttaccggtt
     7021 tcgtacatcg tttcgatgac cttatccagc gagacgcgcg gtgcactggt gcggcgcaga
     7081 gccatccgtg cggcgttaat cgccttcaca gaggcaatgg cattacgctc aatgcacggc
     7141 acctgaacct gccctgcaac cgggtcgcag gttaaaccaa ggttgtgttc catgccaatt
     7201 tccgccgcca cgcaaacctg ttccgggcta ccgcccagca gttctgcaag acccgcagca
     7261 gccattgaac aggcaacacc cacttcgccc tggcaaccaa cttccgcacc ggaaatagag
     7321 gcgttcattt tatacaatgc accaatcgcg cccgctgcca taaagtaacg ggtatagatg
     7381 tctgggctga ccgattcaat aaagtggtca tagtaagcca gcactgccgg aacgataccg
     7441 caggcaccgt tggttggcgc agttaccaca cgaccaccgg cggcgttttc ttcgttaact
     7501 gccagcgcaa acatgtttac ccagtcaatg acattcatcg gatcgttaga cagtttatcg
     7561 ctggaaacta acatccggcg cagggcagac gcacgacgtg gcacgcgcag cgggcctggc
     7621 agtacacctt cggtgttcat cccgcgatcg atacatgcct gcatggtttg ccagacatgc
     7681 gcgaaatact cgtcgatctc tttcttgctg tgcagcgcca gttcgttctg catagcgaga
     7741 ccagacagcg aatagccggt ttcattacag tacgcgagca gttcggtggc agatttgaac
     7801 ggatacggca cgctcacttc gttggcagca tcctgaccaa agtgttcttc atcgacgata
     7861 aaaccgccgc cgatggaata ataagttttg ctgtagacga cttcatcgcc gttataggcg
     7921 tggatttgca taccgttttc atgcagcggc aggttgccgt tatgaaaacg catcccgttg
     7981 tcgcgcggga aatccacttc atgccgtccc tgtgccagca gcagacgttc gcgctctttt
     8041 acgtcgcgaa taaaaccggg aatactgtcg atatccacgg tggcaggttc gttacctgca
     8101 agacccataa taatggcgat atcggtgtgg tggcctttac ccgtcagcga cagtgaacca
     8161 taaacgtcca cggcaacgcg agtaacgcta tccagtaagc ctttttcgac cagatcatcg
     8221 acgaactgtt tacccgcctt cataggccct acggtatggg aagatgaggg accaatcccc
     8281 accttaaaca tgtcgaatag actaatcacg ataatactcc tgacaaggtg actggacttc
     8341 cagtaacgaa ctaataacag cgcatagtgt aagagggaac gcggcgactg gcttaactat
     8401 tcacatgaat taaactaatc aaaaaccgcg atttaactaa tattgcgatg attgtctcaa
     8461 gtagtgtttt gtagatgtaa agataagtat agtcaggggg tcacaccaat ttgcagcgcc
     8521 agctcacgaa ttatgcctgc ggtcattccc catacaaaat actgttcgta ccaggatagc
     8581 catacccgat gtgaatcgcc acggcggtag atatctaaag ggtgataacg acccagatgt
     8641 aatgcctggg cgagcggcat ttcaaacacc gccgagactt catcttcact ggcgcgatac
     8701 ggcagatcgg gcgggataat gccgaccact ggggttactt gatagccagt tacgctatcg
     8761 acgggcggca gcacgccgat aacttcaacg gcggaaggcg gtatagcgac ctcttcttca
     8821 gcttcgcgca gcgcggcggc gataactgat gcgtccgtgt catcgactgc acctccaggg
     8881 aatgccactt gtccagcgtg tttacgcaga tgaattgaac gctgagtcag caacaacccc
     8941 ggttgcggtc gacggacgat ggggattaac acggcagcct gacgatgatt taggggttcc
     9001 cggttaattt gcgggcgcaa aagttgaaag cgagataaaa aatcatcaag cgtcaggcta
     9061 cggtattcca cgtcttactt ctccagttgg cgtaatatct tattaacttt atcaaaagtt
     9121 tcctgatatt ccgcttcttc ctggctatcg gcgacaattc caccgcccgc agagcagtat
     9181 atttgtccgt taatggcagt cagcgtgcgg atagtaatgc tggtatccat gttgccgcaa
     9241 aagctcaaat agccaatgct gccgcaccag gcattacgtc gctgcggttc cagttcgtcg
     9301 ataatttcca tagcccgtac tttcggagcc ccggttattg agccaccagg aaaagctgcg
     9361 cgcagcagat cgctggcgtg taactgttct ggtagtcgcg ccgttatagt gctgaccaga
     9421 tgatgcacgg cagggaaggg ttccaccacg aagagctctg gtacttttac cgaaccggct
     9481 acggcaacac gaccgatatc attacgcatt aaatcgacaa tcatcagatt ttcggcacga
     9541 tctttcgctg agttcgccag tttttctgct tgtttgctat cttcctgagg atcgggcagg
     9601 cgtggtagcg tgcctttaat cgggcgagtc tggatttcac tattatcaca aagaataaac
     9661 cgctctggcg aaaggcttaa aattgcaccc tgttcaagac gtaaaaaagc gctaaatggc
     9721 gcgcggttgg cctgattaag ctgaaggaat gcctgccatt catcgccaga ataggtcgca
     9781 tgaaaacgct gggcgagatt cacctgatag caatcaccgc tgtgcagata ttcctgtacc
     9841 tggcgaaatt tttcgccgta ctgctcgtgg gtcatattgg attgccagtc ggaagtgagc
     9901 gtgaaatctt cctgcggcga gaattgctgg ctttccagcc aggcccgacg ggcattgaca
     9961 tcattatgac tcagcaaaga aactgtatga cgctggtggt cgacaatgag cgcccaatcg
    10021 tagataccca ctgccatatc cggcagaacg atatcttgtt ccgcaatttc tggcagtgac
    10081 tcaaaacggc ggcccagatc gtagccaaac aaccccagtg cgccgccctg aaatggcaaa
    10141 tcttcgttat gcgttgggcg aatgtctgcg cgatccagca cctgctggag cacctgtagc
    10201 gggtcatcag tggtcgttgt gcgtttttcg ctttcactaa caacggtttc tttacctaaa
    10261 gtggttaaag tgcaaatcgg atcggcgacc acaatatcaa agcggctata cggatgatcg
    10321 gcatagccgg agtgtaaaag catcgcccac ggcaggtggc ttaagcggga gaaataaaat
    10381 tcagcggcgt cctgacgcca gggtaaagta atcacagtgg gagataacgt cttcatcagt
    10441 cctgactcta ctggctatgt gccgggtagc atgaaataat tagcgccaac aatttagcag
    10501 gagttaacaa tgtttgcagg tttaccttca ctcatccatg aacagcagca aaaagctgtc
    10561 gagcggatcc aggaactgat ggcacaaggg atgagtagcg gccaggcaat cgcgctggtg
    10621 gcagaagagc tgcgtgccaa ccactccggc gagctaatcg tggcgcgttt tgaggatgaa
    10681 gacgagtaac acactaatcc gtatcgattt cgttatagaa gccgatgatc atttgattgt
    10741 gagaaaaagg acgcttttta tatgtgatta aaagcgtcct gacccattta tgctgatata
    10801 aatttaactc agaccagtgc ttttagttct tcctgcatat atgttgaaaa gtattcagga
    10861 tttttaatca cagagcgttc acctgtctcc attttttttg cccatccaac cattttttcc
    10921 gtgaacccag ggttccagcc ttcctggtca gctcgcgccg taatatcagc ggcactcgcg
    10981 ggtactccca gttcttttaa atatttaaag attcccttcg ccgtgctttc attcatcgaa
    11041 cttggtgttg gcgctgatac atccatggca ccaatgaaat ccagggcttt atctattaca
    11101 gcaggcatat tcttatcctt aaaattaacc atgttatcga taaggctata tatgacctta
    11161 atttgcatct aatatcaaag ggattataac gatatggcac gagttaacct gttgacattc
    11221 aggtgtttta aaggtagcag ctcatggtgt gttatccagt aagatggaga aaattaacgg
    11281 tcagcccgca cgctataacc agattgttca gctaagaaag aaaaccagag ttgctaatca
    11341 aagggcgaac acaacggcat ggttatgttt atttatatag ttagttttat agataaaatt
    11401 gaaattataa atataaataa tgaattagca gaagcatcaa cagtaatatc ttattctctg
    11461 gtcgatttat gtaaaaccga ccagtattat cgataaatta tcgtctgact tattttgctt
    11521 cgataaagct tacaccgcag ccacaatctt aatttcaact ttatacttcg ggttcatcaa
    11581 acccgcttgt accgtgcagc gcaccggcgc atgacccgcg acaacccagg catcccacgc
    11641 tttattcatc gccgcgaagt cgtttttatc ggccaggaaa atggtggcat ccagaatgct
    11701 cgatttattg ctgccctgtt tttccagcac ggcgtcaatc tgtgccagcg tgttggcggt
    11761 ttgctcaaag gcatcggcgt cgaggttttc cggtacacca gtgtagtaga gcgtgttgtt
    11821 gtggattact acatcagacc agcgggcttc agcatcgata cgaacgatag tcatcatttt
    11881 gtcctcatta tacttccatt ttcgtgctgc aaagactgcc acaatgtgag gttgccgtca
    11941 ccacttcaac tggcgaaagc gccctcatcc tgacataatc cctgttcaaa tcaacagggg
    12001 gtagtgtgac ggacgatttt gcaccagacg gtcagctggc gaaagcgata ccaggcttta
    12061 agccgcgaga accacagcga cagatggcgg tagccgtcac ccaggcgata gaaaaaggcc
    12121 agccgctggt ggtggaagca ggaaccggta cgggcaaaac ctacgcttac ctggctcctg
    12181 cgctgcgggc gaaaaagaaa gtcattatct cgaccggctc aaaagcgttg caggatcagc
    12241 tctacagccg cgatttgcca acagtctcaa aggcattgaa atatacgggc aacgtggcgc
    12301 tgctgaaagg gcgctcaaac tacctctgcc tcgaacgtct cgaacagcag gcgctggcgg
    12361 ggggcgatct gccggtacaa atcttaagcg atgtgatcct gctgcgctcc tggtctaatc
    12421 aaatagtcga tggtgatatc agcacctgcg tcagcgtggc ggaagattca caggcgtggc
    12481 cgctggtcac cagcaccaac gacaactgtc ttggcagcga ctgcccgatg tataaagatt
    12541 gctttgtggt caaagcacgt aaaaaagcga tggacgccga tgtggtggtg gtaaaccatc
    12601 atctctttct ggcggatatg gtggttaaag agagtggatt tggcgagttg atccctgaag
    12661 cggacgtcat gatcttcgac gaagcccacc agctaccgga cattgccagc cagtattttg
    12721 gtcagtcact ctccagtcga caactgctcg acctggcaaa agacatcacc atcgcctacc
    12781 gcaccgaatt aaaagacacc cagcagttac aaaagtgcgc tgatcgtctt gcccagagtg
    12841 cgcaggattt tcgtctgcaa ctcggtgagc caggttatcg cggtaacctg cgtgagctgt
    12901 tagctaatcc gcaaattcag cgggcatttt tactgctcga tgacaccttg gaactttgtt
    12961 atgatgtggc gaaactgtca ctggggcgtt ccgccttgct ggatgcggca tttgagcgcg
    13021 ccacgttgta tcgcacacgg ctgaagcggc taaaagagat caatcagccg ggctacagct
    13081 actggtacga atgcacttcg cgccatttta ctctggctct cacgccgctc agcgtggcgg
    13141 ataaattcaa agagttaatg gcgcaaaagc ccggtagctg gatcttcacc tcagcaacgc
    13201 tgtcggtgaa cgacgatctg catcatttca cctcgcggct tgggatagaa caggcggagt
    13261 cgttgctatt gccaagcccg tttgattaca gccgccaggc gttactctgt gtgccgcgca
    13321 atctgccgca aaccaaccag ccaggttctg ctcgccagtt agcggcaatg ctgcgaccga
    13381 tcatcgaagc taacaacggt cgttgtttta tgctttgtac ctcgcacgcc atgatgcgcg
    13441 atctggccga gcagttccgc gctaccatga cgcttcccgt tttgttgcag ggggaaacca
    13501 gtaaaggcca actgttgcag cagtttgtca gtgccggtaa tgcgcttctt gtggcaacca
    13561 gtagtttctg ggaaggggtg gacgtgcgtg gcgatacatt gtcattggta attatcgaca
    13621 aattgccgtt tacctcgccg gatgatccac tgttaaaagc gcgcatggaa gattgtcgtt
    13681 tgcgcggtgg cgacccgttc gatgaagtgc aactaccaga tgccgtcatt actctcaaac
    13741 agggggtagg tcgactgatt cgcgacgccg acgatcgtgg cgtgctggtg atttgtgact
    13801 atcggctggt gatgcgtcct tacggcgcga cgtttctcgc cagtctgccg cccgcgccac
    13861 gcacccgtga cattgcccgt gcggttcgtt tccttgcgat accatcctcc aggtaatttg
    13921 ttacggagta tggtatgatg cgcgcctttt tattgatcta agcacgaaga cccatgcgaa
    13981 ttctggctat cgataccgcg acagaggcct gctctgtcgc cctgtggaac gacggtactg
    14041 tcaacgctca ttttgagctt tgccctcgtg aacatactca acgaatctta ccgatggtgc
    14101 aggatatcct gaccaccagc ggaacttccc tgactgatat taacgctctg gcttacgggc
    14161 gcggccccgg tagctttacc ggcgtgcgca ttggtattgg catcgcgcaa gggctggcgc
    14221 ttggcgcgga attaccgatg attggcgtct ccacgctaat gacgatggcg caaggggcgt
    14281 ggcgcaaaaa cggcgcaaca cgcgtgctgt cagccattga tgcgcgaatg ggcgaagttt
    14341 actgggccga atatcagcgt gatgaaaacg gtatctggca cggtgaagaa accgaagccg
    14401 tactcaaacc cgaactcgtt catgaacgaa tgcaacagct ttccggcgaa tgggtgacgg
    14461 tagggacggg ctggcaagcc tggccggatc tcggtaaaga gagcgggctg gttttgcgcg
    14521 atggcgaagt gttactcccc gctgcagaag atatgctgcc gattgcttgt cagatgtttg
    14581 ccgagggtaa aacggtggcg gtggaacatg ccgagccggt ttatttgcgt aacaacgtcg
    14641 catggaagaa acttccgggc aaagaatgaa tcttaagagt atgccctgag aaaaaaagga
    14701 gtcgcatcat ggcggttcaa aagaatgtta tcaaaggcat actggcaggt acgtttgcgc
    14761 taatgctgag cggttgtgtc actgtgccgg acgccattaa aggcagcagt accacgccgc
    14821 aacaagattt agttcgggtg atgagtgcgc cgcagctgta cgttggtcag gaggcacgct
    14881 ttggtggcaa agtggttgcg gtacaaaacc agcaagggaa aacccgcctg gaaattgcta
    14941 ccgtaccgct ggacagcgga gctaggccga cgctgggaga accttctcgc ggtcgcattt
    15001 atgccgatgt gaacggtttt ctggacccgg tggatttccg tggacaactg gttacggtag
    15061 tcgggccaat caccggtgcg gttgacggca aaatcggcaa tacgccctat aaatttatgg
    15121 tgatgcaagt aacgggttac aaacgttggc atttaaccca gcaggtgatt atgccgcctc
    15181 agccgattga tccatggttt tatggcggtc gtggctggcc ctatggctac ggcggatggg
    15241 gctggtataa tcccggcccc gcgagagtac aaacagttgt aaccgaataa ttgcttgttt
    15301 ttaaagaatg cagaaacagc ggctggtccg ctgtttctgc attcttacgg taaagataaa
    15361 aataaatagt gacgcgcttc gcaacctttt cgttgggtaa ttatcaagct ggtatgatga
    15421 gttaatatta tgttaacggc gtgtatatca tttggggttg cgatgacgac gaacacgcat
    15481 tttagaggtg aagaattgaa gaaggtttgg cttaaccgtt atcccgcgga cgttccgacg
    15541 gagatcaacc ctgaccgtta tcaatctctg gtagatatgt ttgagcagtc ggtcgcgcgc
    15601 tacgccgatc agcctgcgtt tgtgaatatg ggggaggtga tgaccttccg caagctggaa
    15661 gaacgcagtc gcgcgtttgc cgcttatttg caacaagggt tggggctgaa gaaaggcgat
    15721 cgcgttgcgt tgatgatgcc taacttattg caatatccgg tggcgctatt tggcattttg
    15781 cgtgccggga taatcgtcgt aaacgttaac ccgttgtata ccccgcgtga gcttgagcat
    15841 cagcttaacg atagcggcgc atcggcgatt gttatcgtgt ctaactttgc ccacactctg
    15901 gaaaaagtgg ttgataaaac cgccgttcag cacgtaattc tgacccgtat gggcgatcag
    15961 ctatctacgg caaaaggcac ggtagtcaat ttcgttgtta aatacatcaa gcgcttggtg
    16021 ccgaaatacc atctgccaga tgccatttca tttcgtagcg cactgcataa cggctaccgg
    16081 atgcagtacg tcaaacccga actggtgccg gaagatttag ccttcctgca atacaccggc
    16141 ggcaccacag gtgtggcgaa aggcgcgatg ctgactcacc gcaatatgct ggcgaacctg
    16201 gaacaggtca acgccaccta tgggccgttg ctgcatccag gtaaagaact ggtagtaacg
    16261 gcactgccgc tgtatcacat ttttgccctg accattaact gcctgctgtt tatcgaactg
    16321 ggtgggcaga acctgcttat cactaacccg cgtgatattc cagggttggt aaaagagtta
    16381 gcgaaatatc cgtttaccgc tatcactggc gttaacacct tgttcaatgc gttgctgaac
    16441 aataaagagt tccagcagct ggatttctcc agtctgcatc tttccgcagg cggtgggatg
    16501 cctgttcagc aagtggtggc agaacgctgg gtgaaactga ccggacagta tctgctggaa
    16561 ggttatggcc tgaccgagtg cgcgccgctg gtgagcgtta acccgtatga tattgattat
    16621 catagcggta gcatcggttt gccggtgcca tcgacggaag ccaaactggt ggatgacgac
    16681 gacaacgaag taccaccggg tcaaccgggt gagctttgtg tcaaaggacc gcaggtgatg
    16741 ctgggttact ggcagcgtcc ggatgctaca gatgagatca tcaaaaatgg ctggttacac
    16801 accggcgaca tcgcggtgat ggatgaagaa ggattcctgc gcattgtcga tcgtaaaaaa
    16861 gacatgattt tggtttccgg ttttaacgtc tatcccaacg agattgaaga tgtcgtcatg
    16921 cagcatcctg gcgtacagga agtcgcggct gttggcgtac cttccggctc cagtggtgaa
    16981 gcggtgaaaa tcttcgtagt gaaaaaagat ccatcgctta ccgaagagtc actggtgact
    17041 ttttgccgcc gtcagctcac gggatacaaa gtaccgaagc tggtggagtt tcgtgatgag
    17101 ttaccgaaat ctaacgtcgg aaaaattttg cgacgagaat tacgtgacga agcgcgcggc
    17161 aaagtggaca ataaagcctg agcgttaagt cagtcgtcag acgccggtta atccggcgtt
    17221 ttttttgacg cccactaaag agaaaacaat ttgaattacc aaatgattac cacggacgat
    17281 gcgctggctt ctttgtgtga agccgtccgt gtctttccgg cgatagccct ggatactgaa
    17341 tttgttcgta cgcgcactta ttacccgcag ctggggttga ttcaactttt cgatggcgag
    17401 catctggcgc taattgatcc tctcgggatc accgactggt caccgctgaa agcgatcctg
    17461 cgcgatccgt ccatcacaaa atttctccat gcaggcagtg aagatctgga agtgttcctc
    17521 aatgtctttg gcgaattacc acaacccttg attgacacgc aaatccttgc tgccttctgc
    17581 ggacgcccga tgtcatgggg tttcgcttcc atggtggaag agtattccgg cgttacgctg
    17641 gacaagagtg aatcgcgcac cgactggctg gccagaccgc tgaccgaacg tcagtgtgaa
    17701 tacgcagcgg cggatgtctg gtatctgtta ccgatcaccg ccaagcttat ggtagaaacg
    17761 gaggcctccg gctggctacc tgcggcgctg gatgaatgcc gcctgatgca aatgcgtcgt
    17821 caggaagtcg ttgcgccgga agatgcctgg cgtgatatca ccaatgcctg gcagttacgc
    17881 acacgccaac tggcctgtct gcaactgtta gccgactggc gactgcgcaa ggcgcgagag
    17941 cgtgatctgg cggtgaactt tgtcgtgcgt gaagagcatt tgtggtcggt cgcgcgttat
    18001 atgccgggaa gtttaggcga actggacagc ctgggtttat ccggtagcga aatccgcttt
    18061 cacggtaaaa cgctgctggc gctggtggaa aaagcgcaga cattgccgga agatgcctta
    18121 ccgcagccga tgcttaacct gatggacatg cctggttatc gtaaagcgtt taaagcgatt
    18181 aagtcgctga ttactgacgt gagcgaaacg cataagatca gcgccgaatt gctggcatcg
    18241 cgtcggcaaa tcaaccaact gctgaactgg cactggaaac tgaaaccgca gaacaatttg
    18301 ccggagctga tttccggctg gcgtggtgag ctgatggcgg aagcattaca caatttattg
    18361 caggaatatc cgcagtaaaa tcttccgaag ccggactggg cgcgctcagc gccacatccg
    18421 gctttggcaa actacaaatc caacaccagg cgtttaccct tcgcacgcga acaacagatc
    18481 aacatacttt gctggctggc acgctcttca tcgctaaaat actgatcccg atggtcagct
    18541 tcaccttcca gtattgccgt ttcgcaggtt ccgcataccc cttcacgaca taaacattcc
    18601 actttcgcgg ctttattatt ttcaataacc tgcaaaatag tcatctcttc cggcaccaca
    18661 aactcttttc cggaacgggc aagcaccagg gtaaatgcat cgccggtttt gtcttcgata
    18721 gcaaattgct caaagtgcag tgtatcggcg gcgatgtcca gacgcgcagc ttcacttctt
    18781 accgcttcaa ttagcgcctc ggggccacag gtataaacgt gtgtgccagg ttcgatatcc
    18841 gccaataatc gcgctaattc cagtcgtgtt ccggttgatg aatgatgcaa atggactttc
    18901 tcagcctgcg gatgctggac tagctcagca cgaaatgcgc aactttctgg atttcgcgag
    18961 cagtaatgta gctgccagtc gatgtcgctg tgttgcaact ctgccatgtg cgacaggaaa
    19021 ggggtgatac caataccgcc cgcgataaac agatgctttc tggctgaggg aattagcgca
    19081 aacaggttat taggcgttga gatcgttaac cgatcgccca cttttacctg ctgatgcaaa
    19141 aagcgggaac cgccgcgcga gttttcttcc agtcgaacgg caatctgata actagaggtg
    19201 ttatgcggtg aactcagtag tgaatacgca ttgctgtact ggttatcacc atcgctcatc
    19261 tgcactagga tgtgacttcc tccggtgaac gcaggcagcg gtttgccatc ggttgccacc
    19321 agcgtgaagc gtttcacctg ttcggtaagg ggttcaacct ggctcacctg tacttcaaac
    19381 atttgatagt ctgacattgc tgtactccat caaaaatcag cccagcggcg gaaaataccg
    19441 ccgccgatgt cattagtcct taaacacctg cgccagcaga ttatggaaat gggcgatacc
    19501 atgttcggaa atgccactac cgctactgtc ggccatgatg cgcccctgac cacgatagcc
    19561 acgcgatttc agccctttct gtacgctttc aaccagacgt aaatcttccg gatggaacac
    19621 atcgcgatac cactcaatca gcgatttttg ctcgtcggtt aactcttcat tggtgaagta
    19681 aatatcgtag ttttgcaggg tagtttcaga atccaccggg aattcataaa tgaccgtcat
    19741 catccctttg atcggggtga cgttcagcat cgtgcacggc cacagccaga aaccgtggaa
    19801 tgccgcatcc gtaccctctt caaatttaaa cgactgttcg gacggtttgg cgaaaccgta
    19861 ttgcagcgtc cagttaccgt gcatggtgtg ccagtaacga tcaacctgta cggagtcgga
    19921 gaaacctaga tgcgccggac cacagtgata gcactcgaga tagttatcga caatgttctt
    19981 ccagttggca ggcgtgcggg tggtaaagcg ggccgctagt ttcagattgt ggacttccgg
    20041 gcaggcttcc agcactttcg cgcccagacc ggtgtattga ttcacttgaa gtacgaaaaa
    20101 aaaccgggag gacattggat tattcgggtt ctgatgggat tagatttggt ggggcttgca
    20161 agcctgtagc gcaaatttta gtcgttaatc aatgaaacgc gaaagatagt aaaaaattgc
    20221 ttttgtttca ttgaaaatac gaaaaacaaa aacactgcaa atcatttcaa taacagcttc
    20281 aaaaaacgtt caaaaccgat aacaaccagg ctgtaaccaa atgactcata tcacaaatca
    20341 gctcatgccg tttaggtatg ttacatgtgt gattatgtga ggtgaagtat gttttagctg
    20401 gttcatggtt gttatacggc tttttttaca tcttgtggtt cttgtgaagg agctacaaga
    20461 ccttcctgtt cataaatacc atactttgac aaaatctttt tacgagtatc tgttggtaat
    20521 gattcaagca ttttggctgt ttgtaaggtt tgctgcgaaa taccttgcaa atcagcctgg
    20581 atggaatcat cggaagaaaa aatctcaggc ataagagctt caaatccttt ctttctgaac
    20641 aactcaatcg cagaatgcag ttccttgtcc tctaacaatt cgacaattgc gatcagttta
    20701 gttttcaatg tgctgcggtc aggttgagaa tttttattct cttgattatt tttatgctca
    20761 accattggca tgctctgact ttggataaac cactctattg gacgccctgt tgcgtaggct
    20821 atttgtgcta atcgatctaa agacgggtat gtcttgccag acaggtagtt tctgataacc
    20881 ccatcagaaa gtccgcactt agctgcaaac gcccggcttg attctccatt cattgcctct
    20941 tcaaggcgtt ccttgaaacg cattatttcc gggggcataa aaatgcgctc tgcatttttg
    21001 ctttcttttt cacctgtcat gcccaatctc tctgttatat aaagaatttt acagaattct
    21061 ttgatttgca ctggttaaaa taaaaatgcg catagctttt gaattgagca tttttctgcg
    21121 ctatcgtttt tcccgatagc ggtagccggt atcagttacc gaaaacgtta agagagtaag
    21181 gatcgcagaa tgtcagcaaa aaaccaagac tggcacgcag agcagataaa ggctgctgtt
    21241 cgtatgaagg ggaagacact atctcaatta tccagaga
//
LOCUS       DAEBXN010000069        21256 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.069, whole genome shotgun sequence.
ACCESSION   DAEBXN010000069 DAEBXN010000000
VERSION     DAEBXN010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21256
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.069"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            21..794
                     /locus_tag="KJL99_002626"
                     /pseudo
     CDS             21..794
                     /locus_tag="KJL99_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001445615.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
     gene            complement(854..2152)
                     /gene="eno"
                     /locus_tag="KJL99_002627"
     CDS             complement(854..2152)
                     /gene="eno"
                     /locus_tag="KJL99_002627"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HBD4437309.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2240..3877)
                     /gene="pyrG"
                     /locus_tag="KJL99_002628"
     CDS             complement(2240..3877)
                     /gene="pyrG"
                     /locus_tag="KJL99_002628"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HBD4437310.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4105..4896)
                     /gene="mazG"
                     /locus_tag="KJL99_002629"
     CDS             complement(4105..4896)
                     /gene="mazG"
                     /locus_tag="KJL99_002629"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HBD4437311.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     VNEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4992..7226)
                     /locus_tag="KJL99_002630"
     CDS             complement(4992..7226)
                     /locus_tag="KJL99_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HBD4437312.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7274..8575)
                     /gene="rlmD"
                     /locus_tag="KJL99_002631"
     CDS             complement(7274..8575)
                     /gene="rlmD"
                     /locus_tag="KJL99_002631"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBD4437313.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8632..11388
                     /gene="barA"
                     /locus_tag="KJL99_002632"
     CDS             8632..11388
                     /gene="barA"
                     /locus_tag="KJL99_002632"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HBD4437314.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISCVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSIQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQR
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLAGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11620..12960)
                     /gene="gudD"
                     /locus_tag="KJL99_002633"
     CDS             complement(11620..12960)
                     /gene="gudD"
                     /locus_tag="KJL99_002633"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HBD4437315.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDASGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12981..14321)
                     /locus_tag="KJL99_002634"
     CDS             complement(12981..14321)
                     /locus_tag="KJL99_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HBD4437316.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAIFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14323..15675)
                     /gene="gudP"
                     /locus_tag="KJL99_002635"
     CDS             complement(14323..15675)
                     /gene="gudP"
                     /locus_tag="KJL99_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HBD4437317.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPVQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQSTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(16109..16558)
                     /locus_tag="KJL99_002636"
     CDS             complement(16109..16558)
                     /locus_tag="KJL99_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBD4437318.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16576..17358)
                     /gene="truC"
                     /locus_tag="KJL99_002637"
     CDS             complement(16576..17358)
                     /gene="truC"
                     /locus_tag="KJL99_002637"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HBD4437319.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17358..17687)
                     /locus_tag="KJL99_002638"
     CDS             complement(17358..17687)
                     /locus_tag="KJL99_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HBD4437320.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWGLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18309..18854)
                     /gene="syd"
                     /locus_tag="KJL99_002639"
     CDS             complement(18309..18854)
                     /gene="syd"
                     /locus_tag="KJL99_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HBD4437321.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTNLASNLAEFLNQLKPLL"
     gene            18922..19770
                     /gene="queF"
                     /locus_tag="KJL99_002640"
     CDS             18922..19770
                     /gene="queF"
                     /locus_tag="KJL99_002640"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HBD4437322.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19882..21246
                     /gene="ppnN"
                     /locus_tag="KJL99_002641"
                     /gene_synonym="ygdH"
     CDS             19882..21246
                     /gene="ppnN"
                     /locus_tag="KJL99_002641"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HBD4437323.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5229 a         5670 c         5068 g         5289 t
ORIGIN      
        1 ttgttgcagc ccaaccaact attgtcccac aattacagca acaagttacg ggtcttacga
       61 gtagcttaaa ctcacaagaa aagaaagaac tgacgcacaa gttagaatct attttcaata
      121 atacccaagt gcaaattgct gtattaattg tttccacaac caaagatgag acaattgaac
      181 aatatgctac aagagttttt gacaattggc gtttaggaga tgccaaacgt aatgatggga
      241 tactgatcgt tgttgcctag tcggatcgca ctgtccgcat ccaggtaggt tatgggctgg
      301 aagaaaaggt aaccgatgct ctggctggag atatcatccg tagcaacatg atacccgcat
      361 ttaaacaaca aaaattagct aagggattag agttagctat aatcgctttg aataaccaac
      421 tcacttcaca acaccaatat ccggctaatc cttcagaaag tgaatcaacg tcttccactg
      481 atcattatta ctttgctatt ttttgggtat ttgcagtgat gttcttccct ttctggtttt
      541 ttcatcaagg cagtaatttt tgtcgcgcat gtaaaagtgg agtctgtatt tcagcaatcc
      601 atcttttaga tttattcctg ttctccgata aaactttttc cattgctgta ttttccttct
      661 tttttacttt caccatattt atggtcttta cctgtttatg tgtacttcag aaaagagcat
      721 ctggtagaag ttatcattca gacaacagcg gttccgctgg aggctcaagt ggcggcgcat
      781 ccggccgctg gtaactccac cataaaaaat gccagcccga aggctggcat tatcaaatca
      841 gataaagtca gacttatgcc tggcctttga tctctttacg accgttgtac ggtgcttttt
      901 cgcccagagc ttcttcgata cgaatcagct ggttgtattt agcaacacgg tcagaacggc
      961 tcatagaacc agttttgatc tggcctgcag cagtaccaac agccagatca gcgatggtag
     1021 catcttcagt ttcaccagaa cggtgagaga taactgcagt gtagccagca tctttcgcca
     1081 tcttgattgc agccagagtt tcggtcagag aaccgatctg gttgaatttg atcaggatgg
     1141 agttagcgat acctttttcg ataccttctt tcaggatctt ggtgttggtt acgaacaggt
     1201 cgtcaccaac cagctggatt ttgtcgccca gaactttggt ctggtatgcg aaaccgtccc
     1261 agtcagattc gtccagaccg tcttcgatag aaacgatcgg gtactgtttg gtcagttctt
     1321 ccaggaagtg agtgaattct tcagaggtga acgctttgtt gccttcgcca gccagaacgt
     1381 atttaccatc tttgtagaat tcagaagctg cgcagtccat cgccagagtg atgtctttgc
     1441 ccagttcata accagcagct ttaacagctt cagcgataac agccagagct tcagcgttgg
     1501 aacccaggtt cggcgcatag ccaccttcgt caccaacagc agtgttcatg cctttcgctt
     1561 tcagaacttt tgccaggtga tggaaaactt cagaacccat gcggatggct tctttcacag
     1621 ttttcgcgcc aaccggctga atcatgaatt cctggatatc aacgttgttg tcagcgtgct
     1681 caccaccgtt gatgatgttc atcatcggaa ccggcatgga gtatttgccc ggagtgccgt
     1741 tcagttcagc gatgtgctcg tacagcggca tacctttagc agctgcagca gctttggcgt
     1801 tagccagaga tacagccagg attgcgttcg cgccgaattt ggatttgttt tcggtgccgt
     1861 ccaggtcgat catgatcttg tcaatgccag cctgatcttt agcatctttg ccaatcagcg
     1921 cctgagcgat cgggccgttt accgcagcaa cagctttggt tacgccttta cccaggaaac
     1981 gggatttgtc gccatcgcgc agttccagag cttcacggga accagtagaa gcacctgacg
     2041 gagcagctgc cataccgacg aaaccaccct ccagatgtac ttcggcttca acagtcgggt
     2101 taccacggga gtcgatgatt tcacgaccga tgatttttac gattttggac attaggtttt
     2161 cctcaagtca ctagttaaac tgaaactcca gacaaacaac gcgtacccaa ggtacgcgtt
     2221 gccgttctaa cttttttact tacttcgcct gacgtttctg gaactcgctg gcggctttca
     2281 caaagcctgc aaacagcggg tgaccatcac gtggagtaga agtaaactcc ggatggaact
     2341 ggcaagccac gaaccacggg tgattcggaa cttcgatgat ctcgaccaac tgatcatccc
     2401 cggaacggcc cgcaacgcgc agacctgcat cttcaatctg tttcaacagc atgttgttga
     2461 cttcgtaacg gtgacgatga cgctcaacaa ttgtcggcgc attgtatagc tggcgaacca
     2521 ggctatcgtc aaccaactgg cactgctgtg cgccgagacg catggtaccg ccgagatcgc
     2581 tcttctcgct acgaacttca acgttgccgt tttcatcgcg ccactcggta atcagcgcca
     2641 caaccgggta cttacagtct ggcacaaatt ctgtagagtt ggcgttctcc atgttggcaa
     2701 catggcgagc gtaatcaatt aacgccacct gcatacccag gcaaatgccc agataaggaa
     2761 tattgttctc acgcgcaaaa cgcgcggtcg taatcatgcc ttctacgcca cgatagccga
     2821 aaccgccagg tacgaggatt gcgtccagac ctttaaggat ttcaacgccg cgcgtttcaa
     2881 catcttgtga atcgatcagt ttgatgttga cgctgacacg attcttcagc ccaccgtgtt
     2941 tcagtgcttc gatcactgat ttataagcat ccggcagttc aatgtacttg ccgaccatac
     3001 cgatggtgac ttcacttacc ggattcgctt cttcgaagat aacctgttcc cattcggaca
     3061 gatttgcttc cgggcagttt aagctgaatc gtttacaaat ataatcgtcc agcccctgag
     3121 atttcaacag gcccggaatt ttatagatgg aatcgacgtc tttcagagaa ataaccgctt
     3181 tttccggaac attacagaac aatgcaatct tcgcacgttc gttcgccggt acagcgcgat
     3241 ctgaacgaca aatcaggatg tcaggctgga taccgatgga gagcagctct tttacagagt
     3301 gctgagtcgg tttggttttg acttcaccag acgctgccat gtacggcacc agcgtcaggt
     3361 gcataaacag agtgtgctca cggccaattt caacagccat ctggcgaata gcttcgagga
     3421 acggcaagga ttcgatatca cctactgtac cgccgatttc taccagtact acgtcatgac
     3481 cttcgccacc ttccagcacg cgctctttga ttgcgttagt gatgtgcgga ataacctgca
     3541 cggttgcgcc gaggtagtca ccgcggcgtt ctttacgcag aacgtcagag tagatacgac
     3601 ccgtggtgaa gttgttgcgg cggctcattt tggtacgaat gaaacgctcg tagtgcccca
     3661 ggtccaagtc ggtttcagcg ccgtcttcag taacgaacac ttccccgtgt tggattgggc
     3721 tcatagtacc tggatcgacg ttgatgtaag gatccagttt catgatggtc acattgaggc
     3781 cacgggcttc aagaatggct gcgagggagg ctgcggcaat gcctttaccc agagaggata
     3841 cgaccccgcc ggtcacaaaa atatagttcg ttgtcatgct gaacctgaga agttaggttg
     3901 aaaagacgat ggaataacca agacgggaaa gcagtatacc cgaacatgac ctgtgccaca
     3961 aactttcatt atccctcctc ttcgccagcg cactattgaa atcaggagtg agaaaatagc
     4021 ccctttgggg taaatgtttt tgacgcaaat caagcgcttg tcatttaaaa aatgacacaa
     4081 atggcgcttg accgcgtaat tcccttagag atcaatttcc tgccgtttta cctgttgcca
     4141 gacttcttcc attgtttcga ggtcaacacc tgtcatttcc agtccacgcg cggcaacaat
     4201 acgctccact tcgcgaaaac gacgctcgaa tttttcgttc actttttgca atgcgatttc
     4261 tgctttcgtc cctaaatggc gagccagatt aaccgtggca aacagcaggt cccccatttc
     4321 ctcctccagt ttagcctggt cgacaacagc ctgccgcgcc tcgtacatca cctcgtcgat
     4381 ctcttcgtag actttatcaa ccaccgggcc aagcgtcgtc cagtcgaagc caacgttggc
     4441 gcaacgtttc tggatttttt gcgcccgcat taaagccggt aaactacgag gaatatcgtc
     4501 cagcgccgaa tgctgcgctt tctgcgcgcg ctcttcggtt ttgatttgct cccaacgggc
     4561 aagcacttca ctactgtttt cggcagaact atcagcaaaa acatgcggat ggcgacgctc
     4621 taatttatcg ctaatagcag cgcaaatatc attaaagtca aagcgccctt cttcctgagc
     4681 catttgcgcg taaaacacca cctggaatag cagatcgccc agttcaccgc gaagatcgtc
     4741 aaaatcttca cgggcgatgg cgtccagcac ttcgtaggtt tcttcaaggg tgtaaggcgc
     4801 aatggtggca aatgtctgct ctttatccca cgggcagccg ttttccggat cgcgcaggcg
     4861 ctgcataata gtgagcaaac ggtcgatttg attcattgaa ttgtcctgaa aattgcgggt
     4921 ctgtcaggtg taataccttc gagtaaaaga tatcgctgga tacggctgac gccatatcca
     4981 gctatgtaag actaactccc gtgcaaccga cgcgcgtcga taacatccgg cacctggttg
     5041 agtttaccca gcacgcgccc cagcacttgc aggttgtaaa tctcaatggt catgtcgatg
     5101 gtcgccagtt gctgtttggt gtcgctacgg ctggcaacgc caagcacgtt caccttctcg
     5161 ttggcgagaa tggtcgtgat atcacgtaac aacccactac gatcattagc caccacgcgg
     5221 accaccagcg aatatccggc ggagtagctc tcaccccata ccgcgtcaac aatgcgttct
     5281 ggcgcatggg agcgcagttc cgccagttgt tcgcaatcgg cgcggtgtac tgaaataccg
     5341 cgtccctggg taatgaagcc gacaatctca tctccaggaa tcggctggca gcagcgcgcg
     5401 atgtggtgca tcaggttacc aacaccttca accaccacac gaccgttatc tttactgcgg
     5461 ttttgcggtg tgtagctttt ttgctgaagc tgtttcagcg cggcggcgtc ctgctcttcg
     5521 gcactcggct tattaaattg cgattgcagg aagttcacca tctgattgag acggatatcc
     5581 ccgccaccaa tcgccgccag caactcgtcg acatcattga agttgtaacg cggcagcaga
     5641 tgtttttctg cttctttcag gctgatcccc agatgttcca gctcgtcgtc aaggatttgc
     5701 cgcccagcca gaatgttttt gtcacggtcc tgtttacgga accaggcgtg aattttcgaa
     5761 cgcccacggc tggttgtgac gtaaccgagg tttgggttta accagtcacg gctggggttc
     5821 ggctgtttct gggtgataat ttcaatctgg tcgcccattt gcagctggta ggtgaacggc
     5881 acaatgcgcc cgccaatttt tgccccgatg cagcggtgtc cgacatcact gtggatgtgg
     5941 taagcgaagt ccagcggcgt tgatcccgca ggcaaatcaa cgacatcacc tttcggcgta
     6001 aagacataca cccggtcgtc aaacacctga ctacgtactt cgtcgagcat ttcgccggaa
     6061 tcagccatct cttcctgcca cgcaatcagt ttacgcagcc aggcaatccg gtcttcatgt
     6121 cccgaacgtg cgccgccagc tgccgcgccc tctttatatt tccagtgcgc agcaacaccc
     6181 aactccgcat cttcatgcat ctgtttggtg cggatttgga tctcaacggt ttttccaccc
     6241 ggccccagaa ccacggtatg aatagactga taaccgtttg gtttcgggtt agcgacgtaa
     6301 tcgtcaaact catccggcag gtggcgatag tgagtgtgca ctatccccag tgcggcataa
     6361 caatcctgta aacgctcggc gacaatacgt accgcacgca catcaaacag ctcatcgaag
     6421 gcgaggttct ttttctgcat tttgcgccag atgctgtaga tgtgtttcgg acgaccatac
     6481 acttcagctt taacgccttc agctttcatc tcagcgcgca gatggccgac aaactcttcg
     6541 atatagtgtt cgcggtcgag acggcgttca tgcagcagtt ttgcgatgcg tttgtattcg
     6601 gtcgggtgga gataacggaa gcagtaatct tccagctccc atttcagttg cccaatccca
     6661 agacggttcg ccaacggcgc gtagatattg gtgcactctt ttgccgccag tacacgttca
     6721 tcttccggcg catcttttac ttcgcgcaga tgagcaatac gctccgccag tttgatgact
     6781 acgcagcgaa aatcatcgac catcgccaat aacatccggc gaacgttatc gacctgttcg
     6841 gaggaaacag aatcagtgtg cgtcgctttc agctggcgga tcgccgccat atcacgcacg
     6901 ccgtgaataa ggttaacaac cgacttaccg acgctctcac gcagcacatc ttcgctgact
     6961 acgttggcat cagccagagg gaacagcagc gccgcccgca gcgtgtcaat gtccatactt
     7021 aatgtcgaga ggatctccac catctcaaca ccacgccaca ataacagact ggcatccgga
     7081 tgcccctgcg tctgttgcag acaatacgcc caggtttcgg ctaagcactc acacgacttc
     7141 tggctggtaa tacccagact tgcgatccat ttttccggat caaattcacc agccttattg
     7201 atatgtgcac ttcttaccgc aaccatcgtc ctctccttta gggaccagac ctgccgaaat
     7261 cggcaaatcg taactattta acgcgcgaga aaagtaccat cgattccaga tgtcccgtgt
     7321 gtgggaacat atccagcatc gccagtcgcg caatggtata tcctgctttt aataacgctt
     7381 cgctatcccg agccagcgtt gcagggttac aggatacata aactatacga ataggttcca
     7441 gttttataat ttgctgcata acacctgcgg cacctgctcg cgccgggtcc agcaacactt
     7501 tatcgaagcc gtttttcgcc cacggctgct ttgtgacatc ttcttcaaga ttttcgtgat
     7561 aaaacgtcac attctgtaag ccattaagac gcgcattctg ctggcctttt tccaccagcg
     7621 caggaacacc ttctacaccg accacactgg cagcttgtgt cgccaatggc agtgtaaagt
     7681 tgcccatacc gcagaacaga tccagtacgc gatcttcagg ttgcacatcc agccattcca
     7741 acgcacgcgc taccattttt tggttcacac ccgcattgac ctgaataaaa tcgcgcgggc
     7801 taaaagttaa gcgcaacccg tttgagtcat accagggcat ctcaccagag acggtttcga
     7861 gtatctcact atcgggggcg agatacagat ccaggccttc agaatgcgaa aagcgttcca
     7921 gtttttcgcg atctgccgaa cttagcggtg cggtatggcg caaaatcatc agcgtgccgc
     7981 tggttgcctg taccagttca acatgaccaa gatggcgcat agcttgtaag ctgcccagac
     8041 atgccctgac tttgggcagc aatgcttcaa gttggggcgc taaaatgggg cattgtttga
     8101 cgtcgacaat gtcactggag cccgctttgc gaaaccccat ctgaagttgc tgtgttttcg
     8161 gtaagtagtt cagacttaaa cgcgcgcggc ggcgatagcc ccagggaaca tcggcgatca
     8221 cttcagagac atcgtgtttc attaatcggg cgagtgccgc acttttgctt cgctgctgta
     8281 aatccacgct ggcgtgttgt tgctgacagc cgccgcatac gccaaaatga ggacagcgtg
     8341 gcgtttcgcg ttccgggcta tcgcttaacc ggcgtacgac tttagcgcgg gcatactgtt
     8401 ttttatcttc agtaacagca acttccgcgt tttcctgcgg caataatccg gggataaata
     8461 acgttttgcc gttatgtcgc gccacgccct gaccaaaaga gtcgaggtcg ttgactgaaa
     8521 cggttatgat ctgacgcgtc gtcgtgcgtc gttttgcaga gtagaattgc gccattggcg
     8581 agactttctc aatttaacag tgtgacctta attgtcccat aacggaactc catgaccaac
     8641 tacagcctgc gcgcacgcat gatgattctg atcctggcac cgaccgtcct tattggttta
     8701 ttgctgagta tctttttcgt cgtgcatcgc tataacgact tgcagcgtca actggaagat
     8761 gccggtgcta gcattattga gccgcttgca gtttctactg aatatggcat gagcctgcaa
     8821 aatcgcgaat ctatcggtca gttaataagc gtactgcatc gtcgccattc cgatattgtt
     8881 cgcgcgattt cggtttatga tgaaaataac cgactctttg tcacctccaa ttttcatctt
     8941 gatccctcat caatgcagct cggcagcaac gtgccgtttc ctcgccagct cactgtcact
     9001 cgtgacggcg atattatgat cctccgcacg ccgattattt ctgagagtta ctcccccgac
     9061 gaatcgccca gtagcgatgc caaaaatagt caaaatatgt tgggatatat tgcgcttgag
     9121 ctggatctta aatcggttcg cttgcagcaa tataaagaga tctttatttc ctgcgtgatg
     9181 atgctgtttt gtatcggtat tgcgcttatt tttggctggc gcttaatgcg cgatgtaacc
     9241 ggtccgattc gcaacatggt gaataccgtc gaccgcatcc gtcgcgggca actcgacagc
     9301 cgggtggaag gatttatgct cggcgagctg gatatgctga aaaacggtat caactcgatg
     9361 gcaatgtcgc tggctgctta tcacgaagag atgcagcaca atatcgacca ggcgacgtcc
     9421 gatctgcgtg aaacgctgga gcagatggaa attcagaacg ttgagttgga tctggcgaaa
     9481 aagcgcgccc aggaagcggc gcgtattaaa tccgagtttc tggcaaatat gtcacacgag
     9541 ctgcgtacac cactgaatgg tgttattggc tttacccgcc tgacgctgaa aacagaatta
     9601 acaccaacgc agcgcgatca cctgaatacg attgaacgtt cggcaaataa tttgctggca
     9661 attattaatg atgttctcga cttctcgaaa ctggaagcag gtaagctgat tctggaaagt
     9721 attccattcc cactacgcag cacgctggat gaagtcgtta ctctgctggc acattcttct
     9781 cacgataaag ggttggaact gacgctcaat attaaaagcg acgtgcctga taacgtgatc
     9841 ggcgacccac tgcgattaca gcaaatcatc actaacctgg tggggaatgc aattaaattc
     9901 accgagaatg gcaacattga tattctggta gaaaaacgtg cgctgagtaa taccaaagtg
     9961 cagattgaag tgcagattcg ggataccggc attggtattc ctgaacgcga tcaatcgcgc
    10021 ttattccagg ccttccgaca ggctgatgcc agtatttccc gccgtcatgg tggcaccggg
    10081 ctggggctgg tgattacaca aaaactggtt aatgaaatgg gcggcgatat ttcgttccat
    10141 agccagccga atcgcggttc aactttctgg ttccacatta atctcgatct gaacccgaac
    10201 attattatcg aagggccatc catccagtgc ctcgcaggta agcgcctggc ctatgtcgaa
    10261 ccaaactccg cagcagcgca atgcacgctg gatattttaa gtgaaacgcc gctggaagtg
    10321 gtttatagcc caacgttctc cgcgctgcct cccgcgcatt acgacatgat gttgttaggc
    10381 atcgcggtga ccttccgcga gccgctaaca atgcaacatg agcgattagc gaaagcggta
    10441 tcgatgaccg atttcctgat gctggcactt ccttgccatg cacaagttaa tgctgaaaaa
    10501 ctcaagcaag atggtatcgg cgcgtgtctg ctgaaaccat taacacctac gcgcctgttg
    10561 cctgccctga cggaattttg tcatcacaaa caaaacacgc ttttgcctgt aaccgatgaa
    10621 agtaagctgg caatgacagt catggcggtt gatgacaacc ccgctaacct gaaacttatc
    10681 ggcgcattgc tggaagatat ggtgcaacat gtggaacttt gcgatagcgg gcatcaggca
    10741 gttgaacggg cgaaacagat gccgttcgat ttgatcttaa tggatattca aatgccggac
    10801 atggatggca ttcgggcctg cgaactcatc caccaactcc cgcatcagcg acaaacgccg
    10861 gttatcgcgg taacggcgca tgcaatggcc gggcaaaaag agaagctgct tggcgcaggg
    10921 atgagcgatt atctggcgaa accgattgaa gaagagcgat tgcataattt gttgttgcgc
    10981 tacaagcctg gcagcggtat ttcgtctcgc gtcgtgacgc ccgaagtcaa cgaaattgtg
    11041 gtgaacccga atgcgaccct cgactggcaa ctggcactac gccaggcagc aggaaaaacc
    11101 gatttagcgc gcgatatgct gcaaatgtta ctcgatttcc tgcctgaagt tcgcaacaaa
    11161 gttgaggaac agctggctgg agaaaacccg gaaggcctgg ttgatttgat tcataaactg
    11221 catggcagtt gcggctatag cggcgtacca cgtatgaaga atctctgcca gctgatcgaa
    11281 caacagctac gtagcggtac taaagaagaa gatttggagc cggagctgct ggaactgttg
    11341 gacgagatgg ataatgtcgc gcgcgaagcc agcaaaattc tcgggtaacg gacgtcttgt
    11401 cgagtagcaa tcggcttttt atgacgctgg tttcagattg gtgacaaagt gcctgtgttt
    11461 atgccggatg cggcgttaac gccttatccg gcctacaaaa ccaattaaat tcaatgaatt
    11521 aaaaaatgat gtagacctga tacatagcac aacaggctat tttgcgttta gcatcagtct
    11581 caaaccggct ccagatagag ccggttttgg ttttctgtct taacgcacca tgcacgggcg
    11641 cttgttatcg aacgtccagc caggaatcag atactgcatt cccatcgcat cgtcacgcgc
    11701 gccaagcccg tgtttctgat acagctcatg ggctttcatc acttgatcca tatcgatttc
    11761 tacacccagc cccggttttt ctggcacctg taccagcccg cctttgatct caaacggttc
    11821 tttggtcagg cgctgattac cttcctgcca gatccagtgc gtatcaatag cagtaatttt
    11881 acccggtgca gcggcggcaa catgggtaaa catcgccagg gaaatatcga agtggttgtt
    11941 tgagtgtgaa ccccaggtca ggccaaattc atggcacatt tgcgccacac gtaccgaacc
    12001 ttgcatagtc cagaaatgcg gatccgccag cgggatatca acggattgta acgagagcgt
    12061 atggcccatc tgccgccagt cggtggcgat catattggtt gcagtcggca ggccagtcgc
    12121 gcgacggaac tctgccatca cttcacgccc ggagaaacct tgctccgcac cacacggatc
    12181 ttctgcataa gccagcgaac ctttcaggta tttaccaatt ttaattgctt cgttcagcga
    12241 ccaggcaccg ttaggatcga gcgtaatacg cgcctgcggg aagcgttgcg ccagtgccac
    12301 aatagactcg gcttcttctt ccccggccag tacaccgcct ttcagtttga aatcgttgaa
    12361 gccatatttt tcatacgccg cttccgccag gcgcaccacc gcatccggcg tcatcgcttc
    12421 ttcatgacgc aggcgatacc agtcgcatga gtcatccggc tggctttgat acggcagagg
    12481 tgtggctttg cgattaccga cgaagaacag ataaccgagc atttcgactt cgctacgctg
    12541 ttgaccatcg cccagcagcg atgccacgtt tacccccaga tgctgcccca gcagatccag
    12601 cattgccgct tctatcccgg taactacatg aatagtggta cgcaaatcaa atgtctgcaa
    12661 accgcgccca ctagcatcac gatcggcaaa agtattacgc accagcgtca gaacgttttt
    12721 gtattcaccc agcgttttac ctaccaccag cggaatcgca tcttccagcg ttttacggat
    12781 tttctcgccg ccgggaattt cccctacgcc agtgtgaccg gaattatctt tgataatcac
    12841 aatattacgc gtgaagaacg gtgcgtgtgc accactcaga ttcatcagca tactgtcatg
    12901 acccgccacc gggatgacct gcatttcagt aacaacaggc gtcgtaaatt gagaactcat
    12961 atttatgtcc tttttcagaa tcaatgacgg ccgaaaacgg gacgtttacg gtcaaaggtc
    13021 cagccgggga tcaggtactg catcggacct gcgtcgttac gcgcaccgca aggcagacgt
    13081 ttataggcct catgtgcctt ttgtacctgt tcccagtcca gttccacgcc cagaccgggc
    13141 gcatcaggaa cggcaatttt tccgttttta atctccagcg gatttttggt caggcgacaa
    13201 tcgccctcct gccaaatcca gtgggtatcg atagcggtag gattacccgg tgccgccgcg
    13261 cccacatggg taaatatcgc cagagagata tcgaaatggt tgttggaatg gcagccccag
    13321 gtcagccccc agtcgtcgca aagctgcgcc acgcggactg caccggaaag cgtccagaag
    13381 tgcggatcgg caagtggaat atctaccgca ttgagcatca ccgcatgacc catttcgcgc
    13441 cagttggtgg cgatcatgtt agtcgcgacg ggcaagccgg tcgcccgtcg aaactccgcc
    13501 atcacttcac gtccggagaa gccctgttct gcgccgcatg gatcttcggc ataggtaaga
    13561 acatcattca gccctttgca taaagaaatg gcttcatcaa gcagccatgc accgttggga
    13621 tcaacggtaa tccgcgcatc cgggaagctt ttcttcaatg cacgaacagt gtcgatttct
    13681 tgctcgccag gtaacacccc gcccttaagt ttgaaatctt taaagccata gcgatcctgt
    13741 gaggcttccg ccagacgcac aacggcttcg ctgttcatcg ctttctgatg gcgcaactga
    13801 taccactcat ggttgcccgg cgtattttcc agataaggaa gatcggtttt ggtccgatca
    13861 cctatataaa acagataacc gaggacggta atagtctcgc gttgcttgcc tggccccaac
    13921 agttcgcaga ccggaacatt cagcgcctta cctagcaggt caagcaaggc ggcttccagc
    13981 gccgccacgg cattaacgcg caattcaaaa gtccaggcac ctttgccaaa ggtatcaaaa
    14041 tcggctgcct gattaccttt atgtacctgc tgaaccactt tattcaggcg cgcaacttcc
    14101 tggcccagaa ccatcggaat agcatcgacc agtgtttgat aaatcacctc tccgcccggc
    14161 gcttcaccaa tgccggtatg cccggcgtta tcggtgagta ccacgatatt gcgggtgaag
    14221 tatgcgttat gcgcaccacc aatattgagc aacatgctgt catgtccggc gaccggaatg
    14281 actttcatat cagtaataac aggactggat tgtgtcgcca tcttattgcc ccgcgacagg
    14341 tttcagctcg atacgcttga tatcgcccac cagcaccagg tagctcagta ccgcgattag
    14401 ggcatgaaca ccaacataaa tcagcgcccc attaaacgag ccagtcgtgc caacgatata
    14461 accaattgcg attggcgtga cgatgccaga aatgttaccg aacatgttga acaggccacc
    14521 ggaaagaccg ctgatctctt ttggcgcggt atctgccatt actgcccaac ccagcgcccc
    14581 gatgccctta ccgaagaagg ccagcgccat aaagccgatg atcatccact caacgttgac
    14641 gtagttgcag aacaccatca ccatcgacag caacatgccc attacgatcg gtgttttacg
    14701 cgcaatgttc agcgatcccg tgcggcgcat cagccagtcg gaaataattc cacccagcac
    14761 accgccgata aaaccgcaaa ccgccggaac ggaagccaca aagcccgctt tcagaatcga
    14821 catcccacgc gcctgcacca gataaaccgg aaaccaggta atgaaaaagt aagtcagggc
    14881 gttgatacag tactgaccga tataaacgcc gatcatcatc cgtgacccga gcagctgttt
    14941 gatctgcccc cacttcacgc tgaacggcac tttaactttg gtgctttgct gatccatatt
    15001 gatcagcgcg ccacccgcgg cgatatactc cagctctttc tgatttaccc ccggatgttg
    15061 atttggctcg tggatgactt tcaaccagat aaagctgatg acaatgccca gaccgcccat
    15121 aaagaagaag acgtgtgacc agcccacttc atgcgtcagc cagcccataa tcggcgcgaa
    15181 gatcaccgtt gcgaagtatt gagcggagtt aaaaatcgac accgccgttc ccctttcctg
    15241 caccggaaac caggccgcaa caatgcgact gttgccgggg aaagatggcg cttcagcaag
    15301 cccgaccagg aagcgcagag taaacagggc aacgataatg ccgaatccac taaagatatc
    15361 gacgaagcct tgcagcaagg taaacatcga ccagataaag atcgaccaga agtagacgcg
    15421 ttttgaacca aaacgatcca gcaaccaacc accagggatc tgcccgataa cataagccca
    15481 tgagaaggca gagaacacat agcccattcc cacgggatca aggccgatat ctttggccat
    15541 ttccgaaccg gcaatagaaa gcgtagcgcg gtcgccgtag ttgaaggatg tgacgataaa
    15601 caacatcacc actatccagt aacgagcatt cgtgcgtttt tccacactgc tcgcagcctg
    15661 acttaaagaa ctcattgttg cactcctgaa aattcgcgtt agccacgctc actctggact
    15721 gcgacatcgc caggaaatca gaggtgacgt agggtgtttt ttgccgtttt tataggtcgt
    15781 tcgccgaata cggcgcgtgt ttatatctgg caatagcagt ataaaaagcg cgccatagcg
    15841 gctcaccgtg caacaacaca acattaatgc gttcaatgag gcccgatttt ggcattagcc
    15901 ctggacggtg gaatccactt cacggaaatg aaaacaagaa caagaaagga agggtaaaaa
    15961 cgaagaaata aagaagagta tgaaatggat cgcttgactc taggcaaacg ccagtaaaaa
    16021 tccgcgctat gaagcagttt ttactggcat ttgcctgaaa agattcgatt cagcaccgct
    16081 aaaacgacat ttaccgctcg ctgacatatc aggacaacag cgtgccccac tgttcgaccc
    16141 acggatttga ttccgtttcc ggttccgggt tctcactggc gtcaatcaac agcatttcgc
    16201 caacccgctg agcgctctgt tcctgcaaca aggcatcgaa ctgtttgccg ccattgcaaa
    16261 aatttacata actgctgtcg ccgagcgcaa tcacgccata acgcagattc ggctggaagc
    16321 ccagactatc tttgattccc tgaaagagcg gcacaatgct atcaggaagg tcgccctgcc
    16381 cggtcgtgga cgtaaccacc agaacatact tatcctgata gggcagccag tcgcttaatt
    16441 caggatcttc aaataccgtt gctttgtggc cctgtgcggt cagaattgct tctgcttctt
    16501 ccgccactaa cagcgaattt ccgtacatgg tgccgacaaa aataccaatt tccgccatac
    16561 cgttttctcc ctggattagg aacttatctc tccatcctga cccgatggcg cactgaactc
    16621 aaccctttca ttttcaggaa gcagaccgcg ccagccaaat tgtgataacg cctgcatcca
    16681 ggtgtcgtcc aggcccgcgt gaatagtcag cggctcgcca gtaaaaggat gcgtcaatga
    16741 caactgactg gcatgtagca ttaaccgctg gaggccaaaa tgctcagcac cgctgcgatt
    16801 ctggcgtaaa tcgccatgtt tgctatcgcc aataatcgga tgacgcaaat gggcaagatg
    16861 tcgtcgaagc tgatgtttgc gtccggtttt cggctccagt tccaccaggc cgtagcgcgt
    16921 ggtcgggtaa cgtccggtcg ctaccggcat ttctacggtc gccagaccgc gataatgcgt
    16981 cactgctggc tgcgggcctt tatcttcgcg ggcaaattta tcagcgattt tgtccagttc
    17041 ttccaccagt ggataatcca acaccgcttc ttccatcaac cagccgcgca caatcgcatg
    17101 gtaacgtttc tggatttggt gctgttcaaa ctgttgtgcc agcagccgtc cggcctcgct
    17161 ggataatccc atcaacaaca caccagaagt gggtcggtcc agacgatgag cagtaaaaac
    17221 atgctgtcct atctggtcac gcacggtttg catgaccact actttctcgt cgcgatccag
    17281 ccagctacgg tgaaccagcc agccggaggg tttatttacc gcaaccagcc attcatcctg
    17341 atagagtatt tccagcatta gctcgcatca tccgcaaaaa gagcatccag tttttccaac
    17401 tcagccagaa taagcgcgcg ttgcggatgg tccgtcgcca gcgccatttc ataatagggt
    17461 gcaacagcaa aagcgcccgg taacggctgg ttgttattta acaaatcgtg cattcgcggg
    17521 atcagccccc actgcaacca ctccagtggt tccatggtgt ccataaagaa cggttgggta
    17581 ctattaaatt gatgcggctg gggttcatcg tttcgccagt gctgatgttc acgcagtaac
    17641 gcttcaagcg cctgcaactg gagacgaacg cggtcatgag tggtcataaa gcaacctcaa
    17701 taagaaaaac tgccgcgaag gatagcagga ataaaaaaag ggagcactgt attcacagtg
    17761 ctcccggttc gtttcgcagc attccagcta cttttgttgc tccctgctca tccttgacaa
    17821 cttttcctct ggccttgcgg ccaatcgttc atcctgaact attgcttcct gctcacacca
    17881 ccccgatgtg atacttcatc ctgaagtgtc cctggccttc ctgacccacc gaatcatcct
    17941 gaccggttct cattctccat cctggaggtg tcctttaacg cgtcctgcgt catcctcttc
    18001 gcttcatcca gaagcctttc cctgaaacac catcctggtg tgtcctgcag aagtgtcatc
    18061 atcctgatgt tcacttcgtt gtctgactcc ctgtcgacga agatagaatc gtctttttcg
    18121 cgaagtctta caaggcgctt acagacaatg ccttaaggaa aatttcatat gaaagttaag
    18181 aattaacaat caacattttg attttaaatg aaaataacga ttcttatctt tttaagtatt
    18241 cgcttatttc ctggcgatct cctacagagt ctgtaagaga tctctcacaa ccagatgggg
    18301 tatttggatt acagaagagg cttaagttga ttaaggaatt ccgcaagatt tgaggctaaa
    18361 ttcgtccgtt tacgtgttcc cagcgtctct ttgcacactt caccggagag attacacacc
    18421 gagatgacct ctaactcttc ttccagcgtc gcgataaata gcgtgggcgg cagttttagc
    18481 cttttctgag tcaccagatg accaatcaga ttttcctgca cccgacggaa gtcgtcttca
    18541 ctccaggttt gcagcaatgt cagtttgata tcgccaaact gcgcgtgcat atccccggca
    18601 aactgagtcg tataaaaggt atgaattgtg ggttgtatca caatatcaaa agcgcgttca
    18661 accgcgttta cattttgttc cccggtaaac ggctggggtt gccagtatac ggcatcttcg
    18721 gtagttgaaa taatgcacgg tgaaggaaca ccgtacaatt cctcacttaa cggccagctt
    18781 ttatgctctt catgccacgc atcgcagtag cgtgcagtaa aatctttcag ggcttgtgcg
    18841 gtcaaatcgt ccactaattt ctctcttcac ttaaaccaga tacacttgtc gtttagttta
    18901 tctggtttat gacggtgaaa catgtcttct tatgcaaacc atcaggcact tgcgggcctg
    18961 actcttggaa aatcaaccga ttaccgggat acctatgacg ccagcctact gcaaggcgtt
    19021 ccacgcagcc tgaatcgcga cccgctgggt ctgaaagcgg ataacctgcc ttttcacggt
    19081 acggatatct ggacgctgta tgaactttcc tggctgaatg cgaaaggttt gccgcaggtc
    19141 gctgtcggtc atgttgaact tgattacacc agcgtaaatc tgattgagtc gaagagtttt
    19201 aagctctatc tcaacagttt taaccagacg cgttttaata actgggatga ggtgcgccag
    19261 acgctggagc gcgacttaag cacttgcgct cagggtaaga ttagcgtggc gttatatcgt
    19321 cttgatgaac tggaaggcca gccgataggt catttcaatg gcacttgcat tgatgaccag
    19381 gatatcacta tcgataacta tgaattcact actgactatc tggagaatgc caccagcggt
    19441 gaaaaagtag tggaagagac gctcgtcagc cacctgctga aatcaaactg cctgatcacc
    19501 catcaaccag attggggttc gatccaaatt cagtatcgtg gacgccaaat tgacagagaa
    19561 aaactgctgc gttacctggt ctcattccgt catcacaacg agttccacga acagtgcgtg
    19621 gaacgcatct ttaatgacct gttacgcttc tgccagccag agaaattgag cgtttacgca
    19681 cgttataccc gtcgtggcgg tctggacatt aacccgtggc gcagtaatag cgattttgtc
    19741 ccatcgacca caagactggt tcggcaataa attttttctc aattttgcgt gctggattca
    19801 cgcagaaggt tgtgaaaggt catcaggcag ggctattgta atcaaaggga atgacgatat
    19861 tcgtcccata aggagttttt cttgattaca catattagcc cgcttggctc catggatatg
    19921 ttgtcgcagc tggaagtgga tatgcttaaa cgcaccgcca gcagcgacct ctatcaactg
    19981 tttcgcaact gttcacttgc cgtactgaac tccggtagtt tgaccgataa cagcaaagaa
    20041 ttgctgtctc gttttgaaaa tttcgatatt aacgtcctgc gccgtgaacg cggcgtaaag
    20101 ctggaactga ttaatccccc ggaagaggct tttgtcgatg ggcgaattat tcgcgctttg
    20161 caggccaact tgttcgcggt tctgcgagac attctcttcg tttacgggca aatccataat
    20221 accgttcgtt ttcccaacct gaatctcgac aactccgtcc acatcactaa cctggtcttt
    20281 tccatcttgc gtaacgctcg cgcgctgcat gtgggtgaag cgccaaatat ggtggtctgc
    20341 tggggcggtc actcaattaa cgaaaacgag tatttgtatg cccgtcgcgt cgggaaccag
    20401 ctgggcctgc gtgagctgaa tatctgcacc ggctgtggtc caggagcgat ggaagcgccg
    20461 atgaaaggtg ctgcggtcgg acacgcgcag cagcgttaca aagacagtcg ttttattggt
    20521 atgacagagc cgtcgattat cgccgctgaa ccgcctaacc cactggtcaa cgaattgatc
    20581 atcatgccgg atatcgaaaa acgtctggaa gcgtttgtcc gtatcgctca cggcatcatt
    20641 atcttccctg gcggtgtggg tacggcagaa gagttgctgt atttgctggg aattttaatg
    20701 aacccggcca acaaagatca ggttttacca ttgatcctca ccggcccgaa agagagcgcc
    20761 gactacttcc gcgtactgga cgagtttgtc gtacatacgc tgggcgaaaa cgcgcgccgc
    20821 cattaccgca tcatcattga tgacgccgct gaagtcgctc gtcagatgaa aaaatcgatg
    20881 ccgctggtga aagaaaatcg ccgtgataca ggcgatgcct acagctttaa ctggtcaatg
    20941 cgcattgcgc cagatttgca aatgccgttt gagccgtctc acgagaatat ggctaatctg
    21001 aagctttacc cggatcaacc tgttgaagtg ctggctgccg acctgcgccg tgcgttctcc
    21061 ggtattgtgg cgggtaacgt aaaagaagtc ggtattcgcg ccattgaaga gtttggtcct
    21121 tacaaaatca acggcgataa agagattatg cgtcgtatgg acgacctgct acagggtttt
    21181 gttgcccagc atcgtatgaa gttgccaggc tcagcctaca tcccttgcta cgaaatctgc
    21241 acgtaatctc ccctcc
//
LOCUS       DAEBXN010000070        20983 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.070, whole genome shotgun sequence.
ACCESSION   DAEBXN010000070 DAEBXN010000000
VERSION     DAEBXN010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20983
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.070"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(<1..202)
                     /locus_tag="KJL99_002642"
     CDS             complement(<1..202)
                     /locus_tag="KJL99_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HBD4437324.1"
                     /translation="MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLAPELISRCLAE
                     SGTVTLRKRRLPLEMMVWCIVGM"
     gene            complement(347..4807)
                     /gene="mukB"
                     /locus_tag="KJL99_002643"
     CDS             complement(347..4807)
                     /gene="mukB"
                     /locus_tag="KJL99_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HBD4437325.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEVDLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLLGRDEAPSQAS"
     gene            complement(4807..5511)
                     /gene="mukE"
                     /locus_tag="KJL99_002644"
     CDS             complement(4807..5511)
                     /gene="mukE"
                     /locus_tag="KJL99_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HBD4437326.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(5492..6814)
                     /gene="mukF"
                     /locus_tag="KJL99_002645"
     CDS             complement(5492..6814)
                     /gene="mukF"
                     /locus_tag="KJL99_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HBD4437327.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6811..7596)
                     /gene="cmoM"
                     /locus_tag="KJL99_002646"
     CDS             complement(6811..7596)
                     /gene="cmoM"
                     /locus_tag="KJL99_002646"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HBD4437328.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     YQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7732..8511
                     /gene="elyC"
                     /locus_tag="KJL99_002647"
     CDS             7732..8511
                     /gene="elyC"
                     /locus_tag="KJL99_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HBD4437329.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8488..9381)
                     /locus_tag="KJL99_002648"
     CDS             complement(8488..9381)
                     /locus_tag="KJL99_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HBD4437330.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9535..10281)
                     /gene="kdsB"
                     /locus_tag="KJL99_002649"
     CDS             complement(9535..10281)
                     /gene="kdsB"
                     /locus_tag="KJL99_002649"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HBD4437331.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHDDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10278..10460)
                     /gene="ycaR"
                     /locus_tag="KJL99_002650"
     CDS             complement(10278..10460)
                     /gene="ycaR"
                     /locus_tag="KJL99_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HBD4437332.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10512..11744)
                     /locus_tag="KJL99_002651"
     CDS             complement(10512..11744)
                     /locus_tag="KJL99_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HBD4437333.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11781..12767)
                     /gene="lpxK"
                     /locus_tag="KJL99_002652"
     CDS             complement(11781..12767)
                     /gene="lpxK"
                     /locus_tag="KJL99_002652"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HBD4437334.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12764..14512)
                     /gene="msbA"
                     /locus_tag="KJL99_002653"
     CDS             complement(12764..14512)
                     /gene="msbA"
                     /locus_tag="KJL99_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HBD4437335.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14549..16808)
                     /locus_tag="KJL99_002654"
                     /pseudo
     CDS             complement(14549..16808)
                     /locus_tag="KJL99_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(17015..17299)
                     /gene="ihfB"
                     /locus_tag="KJL99_002655"
     CDS             complement(17015..17299)
                     /gene="ihfB"
                     /locus_tag="KJL99_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HBD4437336.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17459..19132)
                     /gene="rpsA"
                     /locus_tag="KJL99_002656"
     CDS             complement(17459..19132)
                     /gene="rpsA"
                     /locus_tag="KJL99_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HBD4437337.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(19243..19926)
                     /gene="cmk"
                     /locus_tag="KJL99_002657"
     CDS             complement(19243..19926)
                     /gene="cmk"
                     /locus_tag="KJL99_002657"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HBD4437338.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQLIEKALQ
                     YARQKLALA"
     gene            complement(20099..20863)
                     /gene="ycaL"
                     /locus_tag="KJL99_002658"
     CDS             complement(20099..20863)
                     /gene="ycaL"
                     /locus_tag="KJL99_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HBD4437339.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         5060 a         5793 c         5104 g         5026 t
ORIGIN      
        1 ccatgccaac aatacaccag accatcattt cgaggggaag acggcgcttg cgtagcgtta
       61 cagtacctga ttcggcaagg caacgagaga tgagttcggg ggcgaggtaa tcccccagag
      121 aagtcagtgg gttacgcaga gaatcgtaac gggataccag atcaagagcc tgtccaatgt
      181 gcataaaaaa atccggaaac aagtgagcgt ttccggattc ttacacagcc actggatcgg
      241 tcaactgatc cttaactgat cggcattacg ccgttatgtg ccattttttt agtgcagaaa
      301 cggagttttc ggaaaaagaa aaggcggcat tgctgccgcc ttaattttaa ctcgcctggg
      361 aaggcgcttc gtctcttcct agaagcgttt cagggagttg cggcgcaaat cctcgcaggc
      421 cgacgacgtg aacatgttcg gtattctgga agactttacg taccagttta taggtggtgc
      481 ctttttccgg actgatattt tccggcgctg cgatgatgag ctgcatttgc aaacgttcac
      541 acaattcaaa cagcgtggcg atagaacgag catccagtcg cgctgcttca tcgaggaaca
      601 gcaggcggca aggagagata tctttaccgc gcaggcggcg agattcatct tcccagcttt
      661 gtaccaccat caccagaatc gacataccgg taccaatcgc ctcaccggtc gacaatgcac
      721 cagactctgc gcgcaaccaa ccatcggaac cacggttaac ctcaacttcc atttccagat
      781 agttgcggta atccagcagt tcttcgccaa tggtctgcgg cgtgcgctgc cccatatcaa
      841 tctgcggatt aagacgctga tacaatttcg ccaacgcttc cgagaaggtc agacggttgc
      901 tgttaaacag atcctgatgc tgctcgtgct gttcagagag cacatccagc agcatggcgt
      961 gcgtttcacg cacgttcacg ttgagacgca cgctgttcac ctgaccaaac gatacgttct
     1021 gcaacccctg gttgagcata cggatacggt tctgctcgcg ctgaatggtt ttgcgaatga
     1081 tgttcgccac gctgcgggaa ctgatcgcca gtttctgttc acgggaggtt aattcttcgg
     1141 tcagacggct aagttcaatc tccatctgtt cgatagcttc caccggatca tcggtacgaa
     1201 taatatcctg acgaatacgt tcacgcagat gctgataaac cgccacgaag aactgaattt
     1261 tacgctccgg acgtttcgga tcttccgaca tgcgcagcac gtcgcgcaga tgttcgttat
     1321 ccgccaccgc cagacgcagc gcacctaacg ccttatccga catggaacgc aaatcatcag
     1381 cggagagata agccagctca cgacggtgta agcggcgctc aacgccgtta tctttcacca
     1441 tgcgcatcac cgcacaccag cccgctttgg cggtcactac ctgctcgcgc atctcaaagt
     1501 aatcccgctc cagcttgcgc agtttgcggg tcaggttgtc catctccgct tcgcagaagg
     1561 taagcgcttt ttccagttga ttgcggcgtg aacggttatt gctcagttgc gcatgcagct
     1621 cgtcacggcg aatacgcgcc cgctcttctg ccccgctatc agcacgcacg ccgatatcct
     1681 gcaattcacg ttgcagatcg ttgagtagct cttttttggt gtcgtaagaa cttttcagcg
     1741 aagccagcac ctggttgtac tgactcaact gcgctgcgtg accgcgcaac gcttcgcgag
     1801 cgcgggtacg ctccgcttcc gcctgttcca gacgttcacg cagtttttcg ttgagatcgc
     1861 tgttaccgct aagcatttct gccgagtcag aatagctaaa gtgcgcacga cgctgcacca
     1921 cttccgtcag ggcaaacgct tgctgacggg catcgcgctg catctgctga gagtacgcgt
     1981 aatcttcttt taactgttcg aactgttccg ggtcgctctg caataccgaa acgatcggtt
     2041 ccagtttcgc cagttgattg ccaaactgct gaacaaaacg cgcggcttcc tgggcttcat
     2101 ccagacgttc gcggatttca tcgacgcgat ccgccaggct gtcatcagcc aacaggttga
     2161 gacgcggcag aatgcggttc agcgccgtaa cgccttcttt cgcctgctca aactgaatac
     2221 gctgctgctg gttatcattt tcatgattac tcaacgcccg ctccagttcg actcgacggc
     2281 tgttcagttg acggatttct gcttccgggt cagactcaaa cgccaccgcc agatgactgc
     2341 cgataaagcg gctgaacgcc tgatgcagac gctgggtttt ctgtacatca aaggagagcg
     2401 tggcgaagcg ttcggaaagc acttcacgct cggcatggag gctttcaata cggctttcac
     2461 gcgcagcacg accaaacagc ggcacttccg ggaaacgtga ataacgccac tgtcgatcgg
     2521 cgattttcac caccaccgct ttttccagct catcaacgct gaacacgcta tcatcgaatg
     2581 actgcggatc cccttcgatc aaatagagat cttccgggca atcggtcaag ccttccaggt
     2641 gttcggttac ctgtgacaga tctggcacca cgatggcgtg gcgtgacggg ccatacagag
     2701 ccgagaagta cggcgcatct tccaggctaa cgtcgtcata aatttctgac agcagcacac
     2761 caccaaaacg ctccgccagc gcgttcagac gctgatcttc agagccgcca ggctggctta
     2821 aacgttcgat ctcttcatcg acggcgtttt tgcgcgcgcc tacttcatcg cgttcaacaa
     2881 tcgcctcgcg ctcacgctcc agcaactgtt gcagatattc agtgacgtcc tggctggagg
     2941 agaactcttc gccgcactgt tcactcaact ggttgagact gttttgcgct gccagccaaa
     3001 ccggtgcacg ctgcatcaaa ctctgaatgc gagactgcag ctgttcctgt tcctgccgca
     3061 gtgccatccg ctcttcacgg gcgttagata cgctatcgga aagagaggca atgcgtgctt
     3121 ccagctcctg atgcagagct tccagttcat caatatcaaa attcttgccc tgacgtttgc
     3181 agaaatctgc cagcagacgc tcagcttctt gctgctcacg cagacgctgt tccagttcgc
     3241 ttaagcgcat ccgcaacggc tgaacctgct ctgccaggtg acgctgatca accccttcgc
     3301 gcaatagttc gcgagcgaca tcccacgcct cgttacgcgc cagcgggccg ttgattgcca
     3361 ccaccagctg ataagcctgc tcaaactggc tgtgcgcggt ttgcgccatg ctcattttct
     3421 gctcaagaga gagcattttt tcagtcgctt ccagctcttt cgcctggaag gtttccagcc
     3481 attcggcggc gctgtcggcg gttaagtccg gcagatggca cagttctttg gcacgattaa
     3541 gcgcagcaat cgcctggtta tactggatcg cgcgcgtttg ctggacgtcc agcgcctgct
     3601 ggtagtcagc aagctggctt ttcagctcgt ccacttccag ttcggcagct tccgcgcgag
     3661 cctcattctc ttcctggcgt tcgatggctt ctgccaccac ttcattttgc tcttccagac
     3721 ggatctgcag ctcatcgaga tccacttcgt agcgttcgat cttctcctgc tgacgcaatg
     3781 cggtttgcac caggttcagg tgatcgctgg ccgcctgata atccgcttcc agatcacctt
     3841 cggcaccgtt gtgctctgcc agctcacgcg ccatatcgac gtgtttgtac tgctcagccg
     3901 ccagttgctg acgcgaagta tgtagctcgc gacgaaactc cagggctttg tcgagatgga
     3961 cacggcgctc gttggcgtga cgcatgtagt ccgccgccac gtagttggta gcttcgctga
     4021 tcagatgctt aaacaggtca cggtccgact gggtgacacg aatcgcttcc agcgtcatgc
     4081 ggttttcacg cagcgccgct tccatgtcct ggaacgcttt acgcacgccg ctgttttccg
     4141 gcaacaggta gtcgcgcaga gaacgggtaa tggcactgga gatcccgcca tacagcgaag
     4201 cttcgatcag acgatagaat ttgctacggt cagatgcgga gcgcagacga cgcgcgatga
     4261 tgcccagatc gaacatcagc gagtggtaat cggtaatgga gttgaactgt ttaaactgca
     4321 cgccttccat cgcctcgagc ttgtctttca gctcgttaag cggcagcacg cgcgcctggc
     4381 gttcgttcag ggtttcggtc accagctgtg tcggctgcac cgacatcggc agtccctgaa
     4441 tggcaaatgg tttgatatcc actttacgat cgcgtccggc aacctgttgc agacgcacac
     4501 cgaccaccac gcgctggtgg cgcgagttaa tggtgtcgag catcgaataa cagacacccg
     4561 ctttcagctt accgtgcaga cctttatcgc gcgaaccgct ggtggccccg gcttccgtag
     4621 tgttacggaa atgcagcagg gtcaggtcgg ggatcagcgc cgtaacgaac gccgccatcg
     4681 tggtggattt acccgccccg ttaccgccgg aaagcgtcgt gaccagctcg tcaaggtcaa
     4741 aagttcgggc aaaaaagccg ttccagttaa tcagcgtcag tgagcgaaat ttaccgcgtt
     4801 caatcattat tcttcctctc cgctatctgg ctgactctct tcggtttcat cgttgagttg
     4861 cagatgattt tcaatcggca ttgcttcgcc atcacgaatc aggcgacgct gtgcttcacg
     4921 gggatcgtcg ccagcacgca catcggctcc gaagcggaac accgattcgg taatgcggaa
     4981 cttgctgctg tcgtggccca taaaccacac catgcctaaa cgacgcagac ggttgagcga
     5041 agaacgtact ttctcctgca acttctgacg gtcaacgtct gaaccggttg aacggttgtt
     5101 caccagtttc agcagtttcg cttcatcggc cagggtgagc agttcgtcgt acagttcctg
     5161 ctgggtgaaa atcccctcat tcgccagccg ttccgggctg agatagagat aacagaggat
     5221 tttcccgacc atcatatcca gttccgacaa gacggaacgc gggataagag tggtggaacg
     5281 tgggcgtaaa tagaagaacc cttccggtgc gcgaataagc tcaacgttat aacgcgcgta
     5341 aaactcttcc agatattcct gaaaatccat caggaatgca tgattatcca gttcgtcgag
     5401 gccaatatgg cggcctgaac gtaaggcgct gtccagcgcc ggaaataacg gattcgccag
     5461 cgcctgcgcc agcttaaccg gcatcacttg ttcaatattt gtcgatgaca tgcgcctgta
     5521 ccttggctcc gtaatcatta atcggctgcc atttcgctgg cagtccggtg aaatctgctt
     5581 gcgctacgcc aagacgtacc gcctgatcaa taacaatacg cgcaacgtca aagtgacgtg
     5641 cacgcggata ctgtgacaga tattcgcgta ccaccagacc aagatccagc ggcacttgtc
     5701 tggttttgta cacggcaagt tgttcttcga tgatcgccgc cagctgttcg cggatctcgt
     5761 taaactcttc gtattccaga tcctcaggaa gttccccagt cacttcttca tcgcgcagtg
     5821 ccatctcttc gtcacgcata tccagcagac gatcggcatt ggcataagtt agcgcccacg
     5881 gctcatcaaa ataggtttgt accgactgac gtaaccgctg agcaaagacg cggtttttat
     5941 ccatatcgat cgcggtacga ataaatttgt gtacgtggcg gtcgtagcca atccacaagt
     6001 cgatggattg ctggccccaa ctgataatac gatcgagttt gctctgcaga tcgaacacca
     6061 gacgatcgac gaaatgcaga tcgtcatggg tcatcgtcgc atcctgaatg cgcaacagat
     6121 tagcctgcaa tttgtcgcct gccgcttcca gcgtatcctg caattcacgc agcgttccgg
     6181 aagtttccga aagcaacaat tcacagctgg aaatcgccgc ccgccagtct ttgttcagca
     6241 actgggcgat atcgtccttc acctgctgct gctgttcgtc catcagacgt tgcgtcaggt
     6301 cgatactgtc gaaaatttct gctaccgaat atttcagtgg cgcatagaca ttacggtgcc
     6361 agtgaaattc atcaccgccc tcttcggcgg catctgctgc gcgtttgagc tcacccgcca
     6421 caatcgacaa ctgcatagaa agacgcagcg tagaaaactc gcgctgacgg atgtagtagt
     6481 cagtaatgcc gatgccgagc ggcgtcagac ggtaaattgc gttcccttcc gcctgctcgc
     6541 tggtaaagcg gttcagcaga cgttgacgca ccatgtcgtt gatcgcgtta ttggcgcgca
     6601 cgccgatggt ttcgctggtt tgctcaaacg catcactcac atggcggaat gcatccacca
     6661 gctcgccttc actcatctca ccatccagac gctcgccgtt cagcgtggca accgccagca
     6721 gaaaagagag tcggtctacc ggcagcgaga tggagaagtc attttttctg gcccaggcaa
     6781 ccagttcggg gactgtctgg gaaaattcac tcatacttta tccttgctct gcggtttgcg
     6841 cgcggtgaca tgaatataac gccctagggt aatatacggt tcctgacggc aataacgcgt
     6901 ttctaattca agtaatgctt catagcagtc gcgctgctgg tacttctcgc gcagataatc
     6961 atgaaacacg cgaacgcctg tcttacccat aatttgccaa ccagcttctt ccagccacag
     7021 ataaacctgc gccgggtcgc gtggataatc tggcgaaagc gtccgttttt tctttttcgg
     7081 cattcccgcc tgcacgtaat caaaattccc ggcgaccatg ttatgcatca acaaaccatg
     7141 cgcattgtag aacattaacg acaacactcc gcctggacgc aacactgacc acagggtctg
     7201 caatacactg cggggatcgg ccacccactc gagcaccgca tggaacaata tcagatcaac
     7261 gggcgtttcc aaatgcgaag caacatcctg agcggcgcaa tgtataaatt gcatgttgtc
     7321 gctcacacct tttgcttctg ccgcctgttt tgcgcggtcg atcatctgcg cagaaagatc
     7381 gcataaaata acctgatgcc cgcgctcggc cattttgatt gcggtctgcc cttctccgcc
     7441 gccagcatcc agcacacgca gtttttgcgg gcccatttcc gccagcacgc gatcgagatc
     7501 ctgccacaga atagcctgtc gaagctgccc tttggtggtg ccgtaaatgt tacgggaaaa
     7561 cttttccgca atatcatcaa aattgcgatc ctgcattgct ttctccaccg gttggctaag
     7621 acgttttctg ataaagacgc tattttgtca cagccacggc gagaatgaac ccgtctggtg
     7681 taatatcgct agcgatccct tgctatatgg ttaaaaaagg aaccagaaag gatgcttttt
     7741 acactgaaaa aagtgattgg caatatgttg ctgccacttc cgctgatgtt gttaattatt
     7801 ggcgctggcc tggcgctgct gtggtttagc cgttttcaaa aaaccgggaa gatttttatt
     7861 agcattgggt ggctggcact cttgttattg agtttgcaac cggtagccga ccgcctgctg
     7921 cgtcccatcg aaagcaccta tccgacatgg aataattcgc aaaaagtgga ctatatcgtg
     7981 gtgctcggag gtggttatac ctggaacccg cagtgggcac cgagctctaa tttaatcaat
     8041 aacagtctgc cacgcctgaa tgagggtatc cgtttatggc gcgaaaatcc gggttcaaaa
     8101 ctgatcttca cgggaggcgt agcaaaaacc aatacggtga gtacagcgga agtaggtgcc
     8161 agagttgcgc aatcgctggg cgtaccgcgc gagcaaatta tcaccctgga tttgccaaaa
     8221 gataccgaag aagaagctgc ggcagtgaaa caggcgattg gtgacgcccc tttcctgctg
     8281 gtgacctccg catcacatct gccgcgggcg atgatctttt ttcagcagga agggttaaat
     8341 ccgctaccag ccccggcgaa tcagttggcc atcgactcgc cgcttaatcc gtgggaacgg
     8401 gcaatccctt ctccggtctg gctgatgcat agcgatcgtg ttggctatga aacgcttggc
     8461 cgcatctggc agtggctgaa aggtccgtca ggcgagccac ggcaagagtg attttgatgc
     8521 cagatcgaag ttgcgccgac taaatcgtcc ggtattaacc agttgcgcaa cttcatccca
     8581 taacacatac aaccaacgcc gccagatgaa cgactccgcc actggcgcgc gttgcaggta
     8641 actccagagc aaatcttccg ccagagaatt atccattagt cggaacagtt cgtattctcg
     8701 cggtgcccaa agcattagtc ccggcccgac catcgccagt aactgatcgc tgcgcgaatc
     8761 tttcagcatg ctgcgtaaac agaagttacc gtgaatcaac acacaattgt cgttaaagcc
     8821 ttcaaataat gccgggagac attcgcgagt gcgaaacagg atccgcttat cctgcatcgt
     8881 cagaccggtg ttattgaact gattgagcgt ggtccatagc acttcaacat gttgccggta
     8941 ccatgagggc cagaaatttt cctgagtatt gtcgaccgcg ccgacgcaac cgcgactgtc
     9001 ctgacggtgc caggccagta aggcttcaac gatttggtct ttgagctgtt cccagcgttc
     9061 tggtgttcgg gctggtgcct ccaccgaaac accacgcatc cgctccagta gcaggacatc
     9121 agggccgggg tgttcttcat gcgtcatcac accataaata gtcggcatac ggacagtccc
     9181 acttcttgcc agcatggtgg ttttccatgc cagttgtcgg gcttttccgg gcgtactaaa
     9241 gcttcttgcc attaacggca ttgggtttcc ctggctgtca tacaaagccc acaacgccgt
     9301 atccgctttt tcattgacgc actcaatacg actgagtttt tcgcccagta aatggcttaa
     9361 ttcggcacgc agctgttcca ttcagattac cctcataaaa gctactgctt tcataatgag
     9421 cgccaatata aaagatgtca cctgaatgag atcaattgtt gaaaagaaaa attgaggaaa
     9481 atcgtaacgg tacgacactc ctcccaaaat tggctgaagt gtcgtgaagt gaaattagcg
     9541 catttcagcg cgaacgcgct caagatcttc aggggtatcc acacctgtgc caggaacttc
     9601 ctgagcaaca gcaacatgga ttttttcgcc gtaccacaga acacgaagct gctctaacat
     9661 ttcgatgtgt tctaacggac ttgcctgcca gttgacgtaa cgacggataa agcctgcacg
     9721 gtagccataa ataccaagat gacgcaggaa gttatcgcca acggtttcaa ggccttctgc
     9781 aaaacgatca cgatcccaag gaatggtggc acgtgagaag tacagtgcat acccttcagc
     9841 gtcgagaacc actttcaccg cattcgggtt aaacgcttct tccgcattgt ggattggcac
     9901 cgccagagtc gccataccca cctgacgctg agcgaggtta tcagcaacct gacgaatgat
     9961 tgtcgcaggg atcatcggtt catcaccctg cacattaacg atcaccgtgt cgtcgctgaa
    10021 tgcgcatttt tcgacaactt ccgccagacg ttctgttcct gactgatgat cggcgcgcgt
    10081 catacatact tcaccgccag cggcttcaac ggcgcgggca acatcatcat gatcggttgc
    10141 cacgatgatg cgctcggcac ctgattcacg cgcgcgttca agaacatgaa caatcatggg
    10201 tttgccgtta atatcaacca atggtttacc gggcagacgc gtggacgcgt agcgcgcggg
    10261 aataatgacc acaaaactca tgatttactc tcatcagcag tcagcacgcg ggcttccgtt
    10321 tccagcaaca ctggaatgcc atcgcggagc gggaaagcca ggttatccag tttgcaaata
    10381 agctcttgtt tttcctggtt ataccaaagt tttccgttgc aaaccgggca ggcaatgatt
    10441 tcaagcaaac gatgatccat agttcctcca gatggatcgg gttatgaatg cataaatctt
    10501 atcataatca tttatgcgac ggggtctatt tcccagccag tgcggcaatc agtaaagaga
    10561 ccttgcgggc agcgtcctaa tgtcacccgc gttgcctgct gccagctcgc gaaatcagta
    10621 atcgcctgac gtaacccttt ttgcagcatt gtcgttggtt taatgccttc ctgtaaccac
    10681 agagagataa cttcaaggat gcctgtctgg cgatgcattt tggcatccat tcgcccaact
    10741 aattgcccac gatgtaataa tggcagaaca aaatagccat actggcgttt cggcgctggg
    10801 gtatagcact ccagccggta gctaaaatca aaaagctgct ctgcgcgttt gcgatcccag
    10861 acaacaggat caaaaggcga aagtaccgcg ctgtgcgttg cagtgagctt tccggccagc
    10921 gcacgctcga gtagcggcag caaatcagca tgcagccaaa gattgcccaa tttttcaaca
    10981 tgcacagcaa tgatttgctg ctgttcagcc ctcgcttcgc gccacgccgc cagtgccgga
    11041 cgtttcagcc gatagtaatc tgccagccac tgttcgcgga atattcccag gctacgcgca
    11101 ctgttatcca gcatgatgat ttctgcttct gtttgcgaaa cgagatcgcg ctcatcatcc
    11161 cagtcaggca tgacacggtg ggttaaatca taaacgcgct ggaagttgcg ccgttcaatc
    11221 accatcacct ttccggcagt aaataaacct tccagatgcc gtttatgcgg cttccattcc
    11281 caccagccgc ttgcaccttt acgaggatgc tcaaaatcgg ctgaacgtac cggcccttta
    11341 tcatgaatat gctgaattaa ctgtgcaatt tccgcctcat gttcctgcat ccaggcgtct
    11401 ttgtatttcc agcccatttt ttcaggtgcc agcatgcggt ggcgaataag acgaaagtcg
    11461 ctacgcggca taaagcaggc ttcatgcgcc cagtattcca ttaattcgcc acgcgccaga
    11521 gactcatcca gccactgggc aggataattt cccagacgac tgaaaagcac cagatatgga
    11581 ctacgggcaa caatattgat ggtatcgatt tgcagcaagg acatgcggga gatcgttgcc
    11641 ggaatatcct ccaacgacgc tcgacggcgg ggtttgttta acaggccttg tgcggcaagg
    11701 tgaagattac gcgcatcagc aagggagagg tgcggcagcg acattcatga ctccatcaat
    11761 cgaacgctgc cgcggcgtaa ctagttgcca gaagccagcg aggttagttg cgtaagcagt
    11821 ttcgctggtt catcacctga aagctgtgcg tctacaggca aataccacca attttcttct
    11881 gcaaaggccc ggcatttcac cgcatctttt tcagtcatta ccagcgtttg cccggcgctt
    11941 accaacgcac tgacatccgc atggttcaaa gactgatgat cggccagcgg tacacatttt
    12001 tccggttgta cgccacacat cttcagcgtg gcaaaaaagc gcggcggatg cccaatcccc
    12061 gccatcgcca ctacatgttc aagctgagca acgtcacaac gcgtaccggt acgtaaattc
    12121 accgcctgac ccggcagcag atgcatgggg atttcaccgc tgcgagggac accgccgttg
    12181 acgattaccg catcaaccga ctttaagcgc cccgctcgct cacgcattgg ccccgccggc
    12241 aaccaccagc cattgccaaa gcgacgcaca ccatcaataa cgacaatttc cacatcacgc
    12301 gccagacggt aatgctgtaa accgtcgtcg gttacgatga tctgcacatc agggtgttgc
    12361 gccagaatgg cttttaccgc atcagaacga acgggagaaa ccgcaacagg cgcatcagtg
    12421 cgttgataaa tcaacacagg ttcatcaccc gcctgtgctg tggtggtatc tgccgacaat
    12481 aacagcggat aagattcagc cttaccacca tatccccgcg atacgacccc cacgcgaata
    12541 ccgcgctgtt gcaactgttc caccagccag acaacgaccg gggtttttcc gttgccgcct
    12601 gcggtgagat taccaaccac gacaaccggt acgggggcac gccaggcgcg cttcagtttt
    12661 agtttatagc aaagacggat cgcgccactc accaggccat acaaccagga gagtggcagc
    12721 aatagccgcc acaaagggga ttcaccagac cagatttttt cgatcattgg ccaaactgca
    12781 ttttgtgaag ttgcgcgtaa acgccgcggt gctcaagcaa atcgttatgc gtaccgcgtt
    12841 ccacaatgac accatcctcg acgaccacga tttcgtctgc cttttcaatg gtagacaagc
    12901 ggtgggcaat caccagagag gtacggtttt tctgcaactc atccagtgcc gcctgaatcg
    12961 cacgttcgga ttcggtatcc agagccgagg tagcttcatc cagaatcaga atcgggctat
    13021 cacgcagcaa ggctcgagcg atagcaatac gctgacgctg accgccagag agcagcacgc
    13081 cgttttcacc aatcactgta tcgagaccgt tatccatctt attgatgaag tccatggcgt
    13141 aggccatccg cgccgcttct tcaatttgct cacggctgta ctgttcagtc cgtgcgtaag
    13201 caatgttgtt agcaaccgta tcgttaaaca gatggacatt ctgcgacacc agagcaacct
    13261 ggttacgtaa cgacgccagg gtatactcgc gcagatcgtg accatccatc aggatttcgc
    13321 cttcatcaat atcgtaaaaa cgcgtgatca ggctggcgat ggttgattta cctgaaccag
    13381 agcgtccaac cagagcaacc gtcttccctg ccggaatttt caggttgatg ttacgcaatg
    13441 cgggtacgtc acgtcccgga taagtaaagg tgacattgcg gaattccacg tcgccagtcg
    13501 cacgctcgat cacgcgctta ccttcgtctt tctcctgctc actgtccaga atggtaaaca
    13561 gcgtctgaca agccgccata ccgcgctgga actgggcgtt aacgttggtc agcgatttca
    13621 gcggacgcat cagtgcaatc attgaagaga aaacaacggt aatcgtaccg gcagtcaggt
    13681 tatccataac acttgggaag ctcgccgcat acagaacaaa cgccagcgcc aaagaggcga
    13741 tcagctgaat gatcggatca gagatggaag aggctgaaac cattttcatc ccctgaagac
    13801 gcattcggtt actgacttta tcgaagcgtt tcgtttccac ttcctgacca ccgaaaatca
    13861 atacttcttt gtggcccttc agcatttgtt ctgcactggt ggtcacttgc cccatggtgt
    13921 tctgcatgtt tttactgatg ttgcgaaaac gcttcgatac tacgcgaatc gcaatcgaaa
    13981 caatcggtgc cagcacaatc aaaatgatcg acagttgcca actgtaatag aacatcatga
    14041 tgaacaggcc gatgatcgac gcaccttcac gcacaacagt aatcagtgcg ccggaagaag
    14101 aagaagcaac ctgttcggaa tcgtaggtga tacgtgacaa cagcgtaccc gttgactgtt
    14161 tgtcaaagaa tgaaactggc attcccatca tgtgaccaaa caggcggcga cgcatggtca
    14221 ttaccacctt tcctgatacc caggagatac agtagctgga gacatagctg gtgataccac
    14281 gtaaaatcat cagtccgatc accaccagcg gcatccacac cagcacggag cgatctgttt
    14341 taccaaagcc atcatcaaga agtggcttaa ggagcgataa catgaaggta tcgctggctg
    14401 cgttgaggat taacgctacg cccgccacga tcagacccgc tttgaaaggc gcaatggttg
    14461 gccacagtcg gcggaatgtc tgccacgtag agagatcttt gtcgttatgc attcaaaaaa
    14521 ccagcatttg ttgaaatagc cgcatattct acccgttatc cactggcacg ccaaaccact
    14581 gatgatacca acgaggtaaa atttgctctc tgaggctgct aatccgccag ccttgtgctg
    14641 aaaaattgac cgtcacttgc ccctgatgtg gagtatcaag ccattgatat cctttctgtt
    14701 gatagcgatg cttaactttg ttagagggca atcgccatgc gttatagcgc gatgccgatg
    14761 cgagtgccac ttttccattc actcgctgaa ttaatggcaa cgatgatgag gtattactgc
    14821 catggtgagg tacctgaagc aatgttgcct gcacttgctg ccagtaacgg cttagcatct
    14881 tttgttcagc tggggcttca atatcaccgg ttagaagaat gctattcgtc ccgtcatcaa
    14941 ccttaaccac acaggaatgg ttatttccct tatcgttgct accttgtaaa ggccagtgcg
    15001 cgctgaaacg caatccttgc cactgccacg cttcgccacg cacacagggc tgatgatgtt
    15061 cccagtttaa cggacttctg atccataaca tcggccatat atgcaatatt gaatccagcc
    15121 ctccccggtg atccagatgt tcatggctca gaataacgcc ttccggttca agattatgcc
    15181 agtggagcca ggggataatc agttgttgcc cactatcccc ttcaggccag tcctgtgtca
    15241 tagagaatcg ctttgccgtt tctggctatc accattgcca gcccttgccc gacatcaagc
    15301 atgtacagct gccactcgtc aggtcgaggt ttttgccaca gcggccagca catcagcaag
    15361 cctccagcca cacacattgc tggcaacgtt cgccaggcgt ttaatcgcca taccaccagt
    15421 aagaaccatg gggaaaatga tagccattgc caacattcaa caatgttgat ccacccttcc
    15481 ggcaaactct ttaaccccca gaaaagtaaa gccaaagacc ggtcggcaag aaaccataac
    15541 ccctgttcca ggattaacgg cccgctaaaa tgcacaacca tcgcggcgag gatcaacgga
    15601 accgtgataa atgtcaccaa gggaattgca aatagatttg caataaacga ggtcagacta
    15661 atgccatgaa atatgacgat ttgcatgggc ataagcagga gtgtgattcc cagttgcaga
    15721 tggatgaggg aaacaactgc acgcaatacc ggcggcagtt gccactcagg acagggaaac
    15781 cactgatacc aaaaaatcag tgccgcgacc gcagcggcag agagccataa actttgcgag
    15841 agaatggcaa caggatccat cagcaaaatt gccgccagac aacatatcca tacatcccag
    15901 ccactccact gtcgcccact taacttaagc attccccacg tagcaagcgc caccacggta
    15961 cgcaatgcag gaggttgcat tcccgtcaac caggcataaa aagcagcaca gcagattccg
    16021 ccaattaatg gtatttgcca gtggatccag cgcccaggca gaaaaatttg tccactgcga
    16081 atgagtccgg cagccagcaa cgccgcaaaa gcgatgtgca atcccgatat cgccattaaa
    16141 tgcgccgttc cagtatcgcg catgatattt ttgatttctt tggggacgga taaccgttcc
    16201 cccataccta aaccaagaat aaccgcattc cacggatagg gttgcagcgt tgtttgtagt
    16261 gacgccagat actgtgcacg caggctacaa ttcggttcaa tgacacttgc ctgcagaaaa
    16321 cggccggtga gcggctgatg ctgggcaatg gcataacgct ggctatcaaa gccgccatca
    16381 ttaagttggc catgaactgc acgaactttg agtttcattg accattgttg tccagcacaa
    16441 accgcttgcg gaagttattc gccatacagc acaaggcctg gcgcagggaa tatacgtcga
    16501 ccttgtagat aagtaatttg accgtaatga gtggtcatgc cgtcagttgc agtgatctca
    16561 acaattgcat cctgcgtcgc gccagttaag gtttctcctg cccacaaaat ttgctttgct
    16621 gataatatgc cccaaacgaa aaacagcaac gtcagcgcga tataacggac ggttttaaca
    16681 gggataaatg ccagtacgca ggcgaagaga gtcagaaacg caagggttaa tgtcctaggc
    16741 aattggggca aaatcagcaa cggaaaaatt ccgcaaatta tgcatacgcc gactgtcgtt
    16801 attttcatgc acccctccct gtttatggaa agtgtgcaac tttgttgagc ggttgtctgg
    16861 tgggaatttt gcgttccccg aggcacattc agaagaattt aacgaaaggg tgaaaactgc
    16921 tcaaaactct gcgaggcagc ttccagttcc tgattagaga attgaaaaaa gcacccgaca
    16981 ggtgcttttc tcgcgttcaa gtttgagtaa aaacttaacc gtaaatattg gcgcgatcgc
    17041 gcagttcttt accaggttta aagtgaggaa cgtattttcc ttccagttct actttatcgc
    17101 cagtcttcgg attacgtccg gtacgtggtg cgcggtagtg caaagagaaa ctgccgaaac
    17161 cgcggatttc aatacgctcg ccctgcgcaa gagtcgaggc catatgctcc agcatctctt
    17221 ttactgcatc ttcaaccgtc ttggcgggaa tgtgcgattg ctgggtggca agtctttcta
    17281 tcaattctga cttggtcatg attcctccgg ttccttaaag gcaaattaag cggctgctgt
    17341 tgctgcattg attaagggcg gccgtagccg cccttagtgc ttgattacag gacgaaacct
    17401 gcaatctgtc aagtaaactc aacaaacttc ggaataaaaa tcccgaagag tcagagaatt
    17461 actcgccttt agctgctttg aaagcttcag ccattgcgtt gttggagaag tttgcatctt
    17521 cctgtttgtt aacagttgcg attgcatctt tctcgtcagc ttcgtctttc gcacgaacag
    17581 acaggctgat tgcgcggttt ttacgatcaa cgccggtgaa tttagcttca acttcgtcgc
    17641 caacgctcag aaccagggta gcgtcttcaa cgcggtcacg ggatgcttca gaagcacgca
    17701 ggtaaccttc aacgccgtca gccagttcta cggttgcgcc tttagcgtca actgcagtta
    17761 ctttaccggt tacgatagcg cctttcttgt tcagagcaac ccagttgttg aacggatctt
    17821 ctgcgagctg tttaacgccc agggagatac gttcacgttc tgcgtcaacc tgcagaacaa
    17881 ctgcagcgat ttcgtcgcct tttttgtatt cacgaactgc ttcttcgcct gcaacgttcc
    17941 aggagatgtc agacaggtga accaggccgt cgatgccgcc gtccaggccg atgaagatac
    18001 cgaagtcagt gatagacttg attttacctt caacacggtc gcccttgttg tgggtttccg
    18061 cgaactgctg ccacgggtta gctttgcact gtttcagacc cagggagata cgacgacgtt
    18121 cttcgtcgat atccagaacc ataacttcca ctacatcgcc aacgttaaca actttggacg
    18181 ggtggatgtt tttgttggtc caatccattt cggaaacgtg taccagacct tcaacgcctt
    18241 cttcgatttc aacgaagcag ccgtagtcag tcaggttggt cacgcgacca gtcagtttgg
    18301 taccttccgg ataacgttta gcgatagcta cccacggatc ttcgcccagc tgtttcaggc
    18361 ccagggatac acgggtacgt tcgcggtcga acttcagcac tttaacagtg atttcgtcgc
    18421 ccacgttgac gatttcgctc ggatgcttaa cgcgtttcca ggccatgtca gtgatgtgca
    18481 gcaggccgtc aacgccgccc agatcaacga atgcaccgta gtcagtgagg ttcttaacga
    18541 tacctttaac ttccatgcct tcctgcaggt tttccagcag ctgatcgcgc tctgcgctgt
    18601 tttcggattc gataacggca cgacgagaaa caacaacgtt gttgcgcttc tgatccagct
    18661 tgattacttt aaattcaagc tctttgcctt ccaggtgcag agtgtcacgc accggacgaa
    18721 cgtctaccag agaacctggc aggaacgcac gaataccgtt cagctcaaca gtgaagccgc
    18781 ccttaacttt gccgttgata acaccggtaa cagtttcagc atcttcgtaa gctttttcca
    18841 gcgtgatcca ggcttcgtga cgtttagctt tctcacggga cagcagagtt tcaccgaagc
    18901 cgtcttctac tgcgtccaga gcaacgtcaa cttcgtcacc tacctggatt tccagctcgc
    18961 cctgggcgtt tttgaactgc tctgccggga tggcggactc agatttcaga ccagcgtcaa
    19021 ccagtactac gtctttgtcg atagcaacaa caacgccacg aacgatagaa cccgggcggg
    19081 tttcgatttc ttttaaggac tcttcaaaga gttgagcaaa agattcagtc atgtttaatc
    19141 ttcaggttta tatttaacgt ccacctggct ccatgccgga tggggttgtt taacataccc
    19201 gctgtcattc cattgcaacg ggggtactgc aaattcggtc gcttatgcga gagccaattt
    19261 ctggcgcgcg tattgtagcg ctttttcaat caattgctca atgcttaagg tggtggaatc
    19321 caacactaaa gcatcggctg ccggaaccag tggcgctacc gctcggttac gatcgcggtc
    19381 gtcgcgttct ttgatctcgg ccaaaaggcg ctcgaagtta acactaaagc ccttctcctg
    19441 caactgtagc atgcggcgat gcgcacgttc ttccgaggag gcgtcaagga aaattttcac
    19501 tggtgcatca gggaatacca ccgttcccat gtcgcggcca tcggcaatca gacctggtaa
    19561 ttcgcgaaac gcgcgttggc gacgcaataa tgcttcacga acgcgtggga atgccgcgac
    19621 ttgtgaagcc gcattcgcca cttcctgagt acgaatttcg ccgctgacat cttccccttc
    19681 gaggatcact tccagattgc cattggtcga cacaaaacgt acatccagat gggatgccag
    19741 cggtaccagc gcatcttccg acgcaacatc aacatggtga tgtaatgccg ccaatgccag
    19801 tacgcgataa attgcacccg agtccagcag atgccattgc aacgcttccg ccatagcctt
    19861 acacaaggtg cctttccctg caccgcttgg gccatcaatg gtaataaccg gggcaattgc
    19921 cgtcatcttt atctccttaa aacaggcgta ccgttaacat aaccgcgcgc attatacgcg
    19981 ccgacgccac aaatcgttaa cgttgtgtgc aaaaaccagt tttgtctgta ttcagatggg
    20041 gatttattgc gcaattatag cgggctggca gaagaccagc ccgaaagatg acaatgattt
    20101 acttaccaga ggcgatacga tcacggatgt gttgcgcacg ctctgttgat ggtgggtgag
    20161 agtcaaacat ggactgggtg cgaccgccat ccaggctagc cagtttttca aagctgccaa
    20221 ccagcccctg ggtgctgatg ccacgtttct tcaacagatc aaaagagaaa tcatctgctt
    20281 cggactcttt atcacgggag tatttagcat taatagcgcc ttctgcgata tcacccaatt
    20341 gtgagcggga aagctgggaa gccacaccgc tggtagctga aatggcatcg cgtgcggcaa
    20401 cgatcgcata agaagctttc atttcagcca gcgagtgacc caacgcgacg tggcccagtt
    20461 catggcccag aacaccttca atttcgttgt cattcatcat gtccatcagg ccactgtaga
    20521 cacgaacaca gccgttcgcc atcgcccatg cgttgacgtc gctggtcata taaaccttat
    20581 agttgaccgg cgtgccatta atattgttac ccaatgcttt ggcgattttt gccagacgtt
    20641 taccgtactt gctggattta cttgcgactt gattgccgct gtccatttcg gcacagccct
    20701 gattggcaat cgcttttgca tcggcatcgc ttaatgttgc tgctttatag gcatttaaac
    20761 cggagctgat agccatattg gtatcaatac cgtgggtatt ttgacaccct gtcagtaatg
    20821 ctgcagaggt cgcaatcgcc agcagtaatt tagtattctt catgtgaatc atccgttgat
    20881 taatcatata taaatattat gtatcaacaa cttacattga caagcgacgc taccattaat
    20941 caaaatgact cacaaggtcc gaaaaaaaac gccgacaatg tgc
//
LOCUS       DAEBXN010000071        20798 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.071, whole genome shotgun sequence.
ACCESSION   DAEBXN010000071 DAEBXN010000000
VERSION     DAEBXN010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20798
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.071"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..256
                     /gene="mliC"
                     /locus_tag="KJL99_002659"
     CDS             <1..256
                     /gene="mliC"
                     /locus_tag="KJL99_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HBD4437340.1"
                     /translation="ERMQTDTLEYQCDEKPLTVKLNNPRQEVSFVYDNQLLHLKQGLS
                     ASGARYSDGIYVFWSKGEEATVYKRDRIVLNNCQLQNPQR"
     gene            315..971
                     /gene="pdxH"
                     /locus_tag="KJL99_002660"
     CDS             315..971
                     /gene="pdxH"
                     /locus_tag="KJL99_002660"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HBD4437341.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            1100..2374
                     /gene="tyrS"
                     /locus_tag="KJL99_002661"
     CDS             1100..2374
                     /gene="tyrS"
                     /locus_tag="KJL99_002661"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HBD4437342.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYDVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            2433..3296
                     /gene="pdxY"
                     /locus_tag="KJL99_002662"
     CDS             2433..3296
                     /gene="pdxY"
                     /locus_tag="KJL99_002662"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HBD4437343.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIEQGPQIVLVKHLARAGYSRDRFEMLLVTADEVWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(3340..3945)
                     /gene="gstA"
                     /locus_tag="KJL99_002663"
     CDS             complement(3340..3945)
                     /gene="gstA"
                     /locus_tag="KJL99_002663"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HBD4437344.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     SVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(4051..5553)
                     /gene="dtpA"
                     /locus_tag="KJL99_002664"
     CDS             complement(4051..5553)
                     /gene="dtpA"
                     /locus_tag="KJL99_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HBD4437345.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPVNY
                     RNLLLTIIGVVALIAVATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKATVA"
     gene            complement(6164..6799)
                     /gene="nth"
                     /locus_tag="KJL99_002665"
     CDS             complement(6164..6799)
                     /gene="nth"
                     /locus_tag="KJL99_002665"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HBD4437346.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     ISVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(6799..7494)
                     /gene="rsxE"
                     /locus_tag="KJL99_002666"
     CDS             complement(6799..7494)
                     /gene="rsxE"
                     /locus_tag="KJL99_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HBD4437347.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGTGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(7498..8118)
                     /gene="rsxG"
                     /locus_tag="KJL99_002667"
     CDS             complement(7498..8118)
                     /gene="rsxG"
                     /locus_tag="KJL99_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HBD4437348.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKT
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWLTHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(8122..9180)
                     /gene="rsxD"
                     /locus_tag="KJL99_002668"
     CDS             complement(8122..9180)
                     /gene="rsxD"
                     /locus_tag="KJL99_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HBD4437349.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLRFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(9181..11307)
                     /gene="rsxC"
                     /locus_tag="KJL99_002669"
     CDS             complement(9181..11307)
                     /gene="rsxC"
                     /locus_tag="KJL99_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HBD4437350.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQTNGTPLRQVLLAQHFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWVDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPVDLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKATRLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAEREARKAQARAKQAELEQTNDAATVAEPRKTAVEAAIARS
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKAAVE
                     AAIARAKARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDP
                     RKAAVAAAIARVQAKKAAQQKVVNED"
     gene            complement(11300..11878)
                     /gene="rsxB"
                     /locus_tag="KJL99_002670"
     CDS             complement(11300..11878)
                     /gene="rsxB"
                     /locus_tag="KJL99_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HBD4437351.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRVMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(11878..12459)
                     /gene="rsxA"
                     /locus_tag="KJL99_002671"
     CDS             complement(11878..12459)
                     /gene="rsxA"
                     /locus_tag="KJL99_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HBD4437352.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLTSICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(12536..12976)
                     /locus_tag="KJL99_002672"
     CDS             complement(12536..12976)
                     /locus_tag="KJL99_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HBD4437353.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEVGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(13062..13277)
                     /gene="ydgT"
                     /locus_tag="KJL99_002673"
     CDS             complement(13062..13277)
                     /gene="ydgT"
                     /locus_tag="KJL99_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HBD4437354.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            complement(13550..13675)
                     /gene="blr"
                     /locus_tag="KJL99_002674"
     CDS             complement(13550..13675)
                     /gene="blr"
                     /locus_tag="KJL99_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HBD4437355.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            13918..14958
                     /locus_tag="KJL99_002675"
     CDS             13918..14958
                     /locus_tag="KJL99_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBD4437356.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            complement(14994..15995)
                     /gene="add"
                     /locus_tag="KJL99_002676"
     CDS             complement(14994..15995)
                     /gene="add"
                     /locus_tag="KJL99_002676"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HBD4437357.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLRYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(16099..17271)
                     /gene="malY"
                     /locus_tag="KJL99_002677"
     CDS             complement(16099..17271)
                     /gene="malY"
                     /locus_tag="KJL99_002677"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HBD4437358.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCNPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            complement(17281..18873)
                     /gene="malX"
                     /locus_tag="KJL99_002678"
     CDS             complement(17281..18873)
                     /gene="malX"
                     /locus_tag="KJL99_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HBD4437359.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESVTRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVEDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            19048..20076
                     /gene="malI"
                     /locus_tag="KJL99_002679"
     CDS             19048..20076
                     /gene="malI"
                     /locus_tag="KJL99_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HBD4437360.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLNGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            20188..>20798
                     /gene="hdhA"
                     /locus_tag="KJL99_002680"
     CDS             20188..>20798
                     /gene="hdhA"
                     /locus_tag="KJL99_002680"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HBD4437361.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLDGQAFAWRCDITSEQELSALADFAVSNLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNSIAPGAILTDALKSVITP"
BASE COUNT         5309 a         5236 c         5336 g         4917 t
ORIGIN      
        1 tgagcgaatg caaaccgata cgctggaata ccagtgtgat gaaaaaccgt tgacggtcaa
       61 actgaataat ccgcgccagg aagtcagttt tgtttacgat aatcaactac tgcatctcaa
      121 acagggcctt tcagcctctg gcgcgcgtta ctccgacggg atctatgttt tctggtcgaa
      181 aggtgaggaa gcgactgtct ataaacgcga ccgcatcgtc ttgaataact gtcagttaca
      241 aaatccacag cgttgagatt tttccagggg cggcgcacaa tagcgtcacc cactgacaat
      301 ccgtaaagaa aaccatgtct gataacgacg aattgcagca aatcgcgcat ctgcgccgtg
      361 aatacaccaa aggcgggtta cgccgccgcg atcttcccgc cgatccatta accctttttg
      421 aacgttggct ctctcaggct tgtgaagcca aactggcgga ccctaccgcg atggtggtcg
      481 ctaccgtgga tgaacatggt cagccttatc agcgcatcgt tttactcaaa cattacgacg
      541 aaaaaggcat ggtgttttac accaacctcg gtagccgtaa agcacatcaa attgaaaata
      601 atccgcgcgt tagcctgctg ttcccgtggc atacccttga acgccaggtg atggtgatcg
      661 gtaaagcaga acgactttcg actctcgaag tgatgaaata ttttcatagc cgcccgcgtg
      721 atagccagat tggtgcatgg gtttcgaagc agtccagtcg catttctgcc cgcggtatcc
      781 ttgaaagtaa attcctggag ctgaagcaga agtttcaaca gggcgaagtg ccattgccga
      841 gcttttgggg cggttttcgc gtcagccttg aacagattga gttctggcag ggcggtgagc
      901 atcgcctgca tgaccgcttt ttgtaccagc gtgaaaatga tgcgtggaag attgatcgtc
      961 ttgcaccctg aaaagatgca aaaatcttgc tttaatcgct ggtactcctg attctggcac
     1021 tttattctat gtctctttcg catctggcga aaagtcgtgt accggcaaag gtgcagtcgt
     1081 tatatacatg gagattttga tggcaagcag taacttgatt aaacaattgc aagagcgggg
     1141 gctggtagcc caggtgacgg acgaggaagc gttagcagag cgactggcgc aaggcccgat
     1201 cgcgctctat tgcggcttcg atcctaccgc tgacagcttg catttggggc atcttgttcc
     1261 attgttatgc ctgaaacgct tccagcaggc gggccacaag ccggttgcgc tggtaggcgg
     1321 cgcgacgggt ctgattggcg acccgagctt caaagctgcc gagcgtaagc tgaacaccga
     1381 agaaactgtt caggagtggg tggacaaaat ccgtaagcag gttgccccgt tcctcgattt
     1441 cgactgtgga gaaaactctg ctatcgcggc gaacaactat gactggttcg gcaatatgaa
     1501 tgtgctgacc ttcctgcgcg atattggcaa acacttctcc gttaaccaga tgatcaacaa
     1561 agaagcggtt aagcagcgtc tcaaccgtga agatcagggg atttcgttca ctgagttttc
     1621 ctacaacctg ttgcagggtt atgacttcgc ctgtctgaac aaacagtacg atgtggtgct
     1681 gcaaattggt ggttctgacc agtggggtaa catcacttct ggtatcgacc tgacccgtcg
     1741 tctgcatcag aatcaggtgt ttggcctgac cgttccgctg atcaccaaag cagacggcac
     1801 caaatttggt aaaactgaag gcggcgcagt ctggttggat ccgaagaaaa ccagcccgta
     1861 caaattctac cagttctgga tcaacactgc ggatgccgac gtttaccgct tcctgaagtt
     1921 cttcaccttt atgagcattg aagagatcaa cgccctagaa gaagaagata aaaacagcgg
     1981 taaagcaccg cgcgcccagt atgtactggc ggagcaggtg actcgtctgg ttcacggtga
     2041 agaaggttta caggcggcaa aacgtattac cgaatgcctg ttcagcggtt ctttgagtgc
     2101 gctgagtgaa gcggacttcg aacagctggc gcaggacggc gtaccgatgg ttgagatgga
     2161 aaagggcgca gacctgatgc aggcgctggt cgattctgaa ctgcaacctt cccgtggtca
     2221 ggcgcgtaaa actatcgcct ccaatgccat caccattaac ggtgaaaaac agtccgatcc
     2281 tgaatacttc tttaaagaag aagatcgtct gtttggtcgt tttaccttac tgcgtcgcgg
     2341 caaaaagaat tactgtctga tttgctggaa ataatgcatt aagtggaaag ggggagtgag
     2401 aaatcactcc ccctggtttt tatacaggga acatgatgaa aaatattctc gctatccagt
     2461 ctcacgttgt ttatggtcat gcgggtaaca gtgcggcaga gtttccgatg cgccgcctgg
     2521 gcgcgaacgt ctggccgctg aacaccgttc aattttctaa tcacacccaa tacggcaaat
     2581 ggactggctg cgtgatgccg cccagccatt taaccgaaat tgtgcaaggc attgccgcca
     2641 ttgataaatt acacacctgt gatgccgtat taagtggcta tctgggatcg gcggagcagg
     2701 gtgaacatat cctcggtatc gtccgtcagg tgaaagcagc gaatccgcag gcgaaatatt
     2761 tttgcgatcc ggtaatgggt catccggaaa aaggctgtat cgttgcaccg ggtgtcgcag
     2821 agtttcatgt gcggcacggt ttgcctgcca gcgatatcat tgcgccgaat ctggttgagc
     2881 tggaaatact ctgtgagcat gcggtaaata acgtcgaaga agcggttctg gcagcgcgcg
     2941 aactcattga gcaagggcca caaattgtgt tggttaaaca cctggcgcga gctggctaca
     3001 gccgtgaccg ttttgaaatg ctgctggtca ccgccgatga agtctggcat atcagccgtc
     3061 cactggtgga ttttggtatg cgccagccgg taggtgttgg tgatgtgacg agcggtttac
     3121 tgctggtgaa actgcttcag ggggcaacgc tgcaggaggc cctggaacat gtgaccgctg
     3181 cagtctacga aatcatggtg accaccaaag caatgcagga atatgagctg caagtggtgg
     3241 ctgctcagga tcgtattgcc aaaccagaac attacttcag cgcaacaaag ctctgaaata
     3301 tctcatttcg gcccacaacg acctgtgggc cgatggctat tactttaagc cttccgctga
     3361 cagcgcgtct tgtacttccg gacgttcagc catacgttgc ataaatgccg caatgtgctc
     3421 taacccttcc agattcagtt tcaccgcgta tgcccagcgc agaaccgtaa acagataggc
     3481 atcagcaatt gtaaatcttt gcccgcagat ccagtgctca tccttcagtg cctcgttcac
     3541 atattgcagc ttcttatcca gctgcgcgcg aactgtcggt ttgtactctt ccggtgtatc
     3601 tgggcgaaac agaggtgtga aacctttatg cagctcggtg gcgatgtaat tcagccattc
     3661 gatggtttta tagcgggaaa tactgtttac cggtgccagc aactggcggt cggggacgct
     3721 gtcggcaaga tactgcataa tcgcaacgcc ttccgtcagc aaagtaccat cgtccagcag
     3781 caatgcaggc acctgcccct tagggttaac ggaaaagtaa tcgtcaccgt tttcgagacg
     3841 ttttttcatt aaatccacac tgacgagcgt aaaatccttt ccgctctcac gcagggtgat
     3901 atgggaagcg agagagcagg cgcccggttt gtagaacaat ttcatcggta actccttttg
     3961 tctgtggttt aaagtatggt agtgcgttgc tgaacaaaaa aaaagccgct aacgaaaagt
     4021 tagcggcttg taaaagagtt tccctgaatt ttacgctaca gtagctttcg ccgctttgtc
     4081 tgcagcgtca tcctgcgtca tgcggtgcag tttaggcgcg gtcagcagca tcagtactgc
     4141 aataacggca gtagcgacac caatctgcaa gaatacgcga ccatagactt ccagtgacat
     4201 cagcgggtcg gtaacgttat ccggcacagc catcataccc gcaacataac caccaatcag
     4261 gtttgcaccg gcagtggtca ggaaccagct acccataatg aagcccatca gacgctgcgg
     4321 aacgagttgt gcaaccattg ccagacccag accagagatc atcagttccc cgatgctctg
     4381 caggccatag cttgcgacca gccagctgac agacacgata cctgcgtcag acgcgaattt
     4441 cgcacccagc ggcagaatca ggaacgcacc agaacacatc accatgccga ttgcaaactt
     4501 ggttggcatc ggcagggtat cacccatctt gttatagata gcggccagaa tcggactacc
     4561 gatgatgatc cagaacgggt tcagtgcctg atactgctca ggttctacgg ccagacccag
     4621 aatggagtgc tcaacgttac gaatcgcaaa gaagttcagt gacgttggca tctggctgta
     4681 cagcacgaag aagatgattg cttcgagcat caggatgaag gcaacgatca ttttacgacg
     4741 cgcagcacct ttcatcgcga aagcttcttt accgaagata accacaatac cgaaggcaac
     4801 aacgcccagc gccatacgcg caacttcctg attgtgcagt agccaggtgg caacagcgat
     4861 cagcgccaca acaccaataa tggtcagcaa caggttacgg tagttgactg gttcaaagtc
     4921 tggttttgaa ccgtactgtt taacccagcg ttggcagaag gcgaagttaa cgatagtgat
     4981 cagcaggcct acaacgctca acgcaaacgc aacactccag ccgtatttcg cggccagcca
     5041 cggggtggct atcatagaga agaaagagcc gatgttgacg gacatgtagt acatggtgaa
     5101 tgcaccgtcc agacgtgggt cgtttttctc atagcatgta gaaagcagag aagacgggtt
     5161 agctttaaac aggccgttac cgaccgcaat agccgccata cccatataaa caataccggc
     5221 gtcgtgacca gaccaggcaa ccagagcata accaatcgcc agcacaatag cgccgagcat
     5281 aattacgcgt ttagtaccca gtaccttgtc acctaaccag ccgccgatag cgaccagacc
     5341 ataaaccagg gcactaaagg aagagaaaag ggtgattgaa tccgcttcag acatacccag
     5401 ttgtttaacc aggtaaacag ccataattcc ttgtaggccg taataaccaa aacgttccca
     5461 taactcaatc gagaagatga gatagaacgc cttcggttgt ttgaaagcgt tcaaactgac
     5521 gctttcagtt ggtttttggt ttgcagtgga cacttttacc tcttttttta catcccatat
     5581 taacgggggt gctttctcgc gttatgtcta aaaaacatac gcgcaattgt tatagtggga
     5641 ggggaaacgg caggtaatgt tcactatctt gcactttcag acaagtcctt tgtaataatc
     5701 tgttacatat aactgaggcg gggaattcaa ccattccggg aaatgttatc cagcgttaaa
     5761 tttttcaaaa aacgtaacgg cagatttttt cactgcattg agagggggat gatggcttgg
     5821 cggcgatatg ttctgccatt tagcgttttt cctagtgaga tagtccagtt tctgaaaaat
     5881 agccagtgta atgttttgca ggtcaatatt tgttgcgtgt gtaaatttaa atcattttaa
     5941 cctgattctt gcaatgtttt tacattgcca cagcatcgtg atcttgatca cgaatgaata
     6001 gaaaatttcg ctattaaaaa aacaattaac ctgcattttt ggttatccgc aaacggatta
     6061 cctgatgata acgctaagca atggcatcat tgacgttaaa aagatgtttt aaaagaggat
     6121 aaagaaaggt tatcaatggg gtaatcggtg ttaccccttt tcttcagatg tcaactttct
     6181 ctttgtattc acaaagatct tcaataatac aagagccaca gcggggcttg cgggcaatgc
     6241 aggtataacg cccgtgcagg atcaaccaat ggtggcagtc gactttaaac tctgccggaa
     6301 ccactttcag tagcttttct tctacctgtt cgacgttttt ccccggcgca aattgagtac
     6361 gattacaaac gcggaaaatg tgcgtgtcga cagcaatagt cggccagccg aatgcagtgt
     6421 ttaatacgac gttggctgtt ttacgaccta cgccgggcag ggcttcaagc gcagcacgat
     6481 cttccggaac ctcgccatta tgttgttcca gtaatatacg gcaggttttg atgatatttt
     6541 ctgctttgct gttataaagg ccaatcgttt tgatataggt tttcacccct tcaacgccta
     6601 gttcaagcat cgctgcaggc gtattcgcca ccgggtagag tttcgccgtc gccttattaa
     6661 cactgatatc ggtcgcctga gcggaaagca gtacggcaat cagcaattca aaaggcgaac
     6721 tgaaattaag ctcggtggtg ggatgaggat tgttctcacg caggcgagtg aggatctcca
     6781 gacgttttgc tttgttcatc agacattccc tgtttcaccg tttggcaatg cacgttcggc
     6841 agctgcttca gcacggcgct ttttcattct ttcatcaatc aggtattttc ctgccagcat
     6901 cagtcccaag ccaataaatg cacctggtgg cagcatcgcc agcaggaaag gggagtcggt
     6961 gtggaaaatc tccacgcgta atacttttgc ccagctacct aacagcgcat ctgcaccgtc
     7021 aaacaacgtg ccattgccga taatttcgcg tagtgaaccc agcacgaaca tggcgcaggt
     7081 tgcgcccgta ccaattgaaa agccgtccag tgccgaaagc gccggacctt ttttggcggc
     7141 gaaggcttca gcgcggccca caacgataca gttagtgaca atcagcggaa taaaaatccc
     7201 taatgattga tacaggccaa aggcgtaggc gttgatcagc atctgtacag cgctgaccac
     7261 cgaggcgatg atcatcacgt aaatgggaat gcggatctcg gctggcgtcc agtgacgcag
     7321 cgtcgaaatg gtcaggttgg tcagcgtcag taccagcgta gtcgcaagtc ctaaacccag
     7381 agcgttagtg gcagtggatg tgaccgccaa tagcggacaa aggccgagca gttggaccag
     7441 tgcagagttg tttttccaca acccctgaac aataacgtct ttaatttcgc tcacgcttta
     7501 ttctccacag gcaggaagtt gagaaagttg tgccggtaac gtctgagcgt acaatccggc
     7561 gcgttttacc gcattaacca ccgcgcgggg agtaatcgtc gcgccggtga actggtcgaa
     7621 atcaccacca tctttcttca ccgcccagtg cgcatcatcc gcaccactga ttttttttcc
     7681 cgcaaaatgg gtgagccagt cagaaaggcg caattcgatt ttatcgccaa gccctggcgt
     7741 ttcgtggtgc tctgtcacgc gcgtgccaag taccgtgcca ttaaaatcgg ctcccaccag
     7801 cagctgaatc gcaccggaat agccatctgg cgcggttgct tccagaacgg cggctaccgg
     7861 tttgtcatcc tgtttggcga tgtaaacccg atgctcacct ttacctaact ctggcgcagt
     7921 taccagatag caactctgtg cgagcgcatt gttatagcgt tcggctggca gcacctgatc
     7981 aaataacgtc ttttgttgca gactggcctg ttcagcaatt gtcgttttgg tcatctggtt
     8041 gatggccgca gttaaccctg tagaacccgc tgcaaatagt gccagtgtaa tgccgtgttt
     8101 tcggatagtt ttcagcatgg tttacccttt gcgatggccg tagacgcgcg gacgcgtgta
     8161 gtaatcgatc agaggaaccg tgatgttcgc cagcaggacg gcaaaagcca cgccgtcagg
     8221 atagccgccg aaactgcgga tcaaccagac taataagccc gccagcgcgc cgaacatcag
     8281 ccgaccacga ttggtcgtag aagcagtaac tgggtcagtc aaaataaaga atgcgccgag
     8341 catagtcgca ccagacagca gatgaatttg cggtgctgcc agtgtttctg gtgagaacaa
     8401 ccagcccaac gttgcgcata acgccagcgt tactaagaag ctgaggggaa tatgccagcg
     8461 aatcgctttc tgccatagca accacacgcc accagccagc caggcgagat ttacccattg
     8521 ccaaccagcg cccgccagaa taccgctgta gatcggatat tgcataatct gttcaaccga
     8581 atgacctgcg cggacagagg ttttgaatgt atccagcggt gtcgcctgac taatgccatc
     8641 aatacctaag cgtagcgtat tcatatcacc accactggcg gtatgaccgc tgaaaataac
     8701 ctgtatggcg tcgataaaac cagggatgtt gaccgcaatt tcatgtggcg gtaaccagct
     8761 ggtcatctgc accgggaagg agatcagtaa gaccacataa ccaatcattg ccggattaaa
     8821 cgggttttgc cccagaccgc catacaactg tttagcgatg atcaccgcaa acaccgtacc
     8881 cagtacgacc atccaccatg gcgctagggg gggaatactt accgccagca ataagcctgt
     8941 cagcaatgct gagttatctt tcaacgttgc ggctaccgac tgcttgcgta gtttgagtac
     9001 gagagcttcg gctaacagag cactgaccga cgccaacagg atctgaacga gagtacccca
     9061 accaaaaaac cgcagttgcg ctgcgattcc tggcacggct gcgagcaaca ccaacagcat
     9121 aatgcgcgat gtctggcgct ggttatgggt ataaggggag ctagctattc tgaataccat
     9181 ttagtcctcg tttacaacct tttgctgggc ggcttttttg gcctgaacgc gggcaatagc
     9241 cgcggcaact gcggctttgc gcggatcgac ctgttcttct ggttccgcat tagcctgttg
     9301 ctgttccagc ttgcgtgctt tggcgcgggc aatagcggct tcgacggcag ctttgcgcgg
     9361 atcaacctgt tcttctggct ccgcattggc ctgttgctgt tccagcttgc gtgctttggc
     9421 gcgggcaata gcggcttcga cggcggcttt gcgcggatcg acctgttctt ctggttccgc
     9481 attagcctgt tgctgttcca gcttgcgcgc tttggcacgg gcaatagcgg cttcgacggc
     9541 ggctttgcgc ggatcgacct gttcttctgg ttccgcatta gcctgttgct gttccagctt
     9601 gcgcgctttg gagcgggcga tagctgcttc aacggcagtt ttacgtggtt cagcaacggt
     9661 tgctgcgtcg ttagtttgct ccagttctgc ctgtttcgct ctggcttgcg ctttacgggc
     9721 ttcccgttct gcaataattg cactgttatc cgggcgttcg cccgctttaa tcacaatagg
     9781 ctgtgtagcc tgggcctgtt tctctttcac ccgcgccaga gcggcagcaa tcgcatcttt
     9841 atctttggct gcaggttgaa cggctgcgct cttatgtcgt tcaaggcgag tcgctttttc
     9901 gcgctccaga cgagcctggc gcgcttcgaa acgcgctttg gcttctgcgg cgcgcttttc
     9961 ttcctgacga atagccgcaa tttcagcttt ttcctgacgg aaatattgca ccaagggaat
    10021 attgctcggg caaacccacg cgcaagcccc acattcaatg cagtcggcaa tgttatgcgt
    10081 ggtagcttta tcgtgttgct gacctttgct gaaccagtac aactgttgcg gcaaaagatc
    10141 cacagggcag gcgtcagcac aggcgctaca ccggatgcag ctttgttctt cctgtggttc
    10201 gccaagttca ttggcagagg gagccaacag acagttggta atctttacga ccgggacatc
    10261 cagccatggc aaggtaaagc ccattagcgg gccacccata atcaccattt gatcggcaga
    10321 ggggcagaat ccggcatcat tcaataaatg acgcactggc gtccccagcc gtgcccagac
    10381 gttgcccggg cgagcgattg cttcgccagt cagggttaca acacgctcgg taatcggctc
    10441 gccatcaata acggcacgtt tcactgcata agcagtgccg acgttttgca ttaatacgcc
    10501 gatatcggat gaacgcccgc catgtggaac ctgcttcccg gtcagaatgt aggttaattg
    10561 tttagcaccg ccagaaggat atttggttgg aatcacccgc agagaaatat cgttagagtc
    10621 cgccagcacc gcgcgcagca tggaaatcgc ctgcggtttg ttatcttcaa tgccgataag
    10681 aatttcgcgt ggctgcagaa tatgcgcaag aatgcgaata ccctctacga cctgagccgc
    10741 gcaatcctgc atcaaacggt catcggcggt aatgtacggc tcgcactcag cagcgttgat
    10801 aatcaacgtt tcaatcttat ctccaccacc ctgcaattta acgcctgtcg ggaatccggc
    10861 accgcccagc ccggcaacac caaactgatg tatgcgctcg attaactctt cgcgactgcg
    10921 agagcgataa tcgacccagc cgtcgcgcgg gatccagcag tcttcaccat cggcatcaat
    10981 aatcacgctt aattcagcta aagctgaagg atgagccgta gagtggggcg caatagccgt
    11041 aacggtaccc gaggtgggcg cgtgaacagg cagcattttg ccgcgaccac gggtaagcgg
    11101 ctggccgcgc aatactttat cgccgacgct aacgcacaac tcaccttcag cgccaatatg
    11161 ctgtttcagt ggaataacaa aatgctgcgc cagtagtacc tggcgcaggg gggtaccgtt
    11221 ggtctgggtt ttcatctccg gtggatggat gccgccgttg aaatcccaga ttttattttt
    11281 tctgaatgca gagaataact taagcatggt gttccacggg aatgatacgc acgggaatgg
    11341 tgttcagatc ccatttccag gagtcaggtg tttctgcgac cggttgcaac gagatgcagt
    11401 gcgtcgggca cggatcaaca cataaattgc agcccgtaca gagatcactc attaccgtat
    11461 gcatgacacg ggtagcgcca acgatggcgt ctaccggaca agcctgaata catttagtgc
    11521 agccaataca gttattttca tcaataaccg ccaccatccg cgcaggcgtt agctcttgcg
    11581 cttcgccatc cagcggctgc ggctcgacat taagcaactc ggcaattttt agcatcacag
    11641 cttcgccacc tggggcgcaa cggttgattt tttcaccgtt acagctgatg gcttccgcgt
    11701 aggggcgaca gccgggataa ccgcactgac cacactggct ctgcggtaag atttcgtcaa
    11761 ttttctcaac gaccggatcg tcttccaccg caaaacggcg ggaggcataa cccagaatgg
    11821 cgccaaacgc caggcccagc aggctcacgg cggcaacggc aatccagata gcattcatta
    11881 caacttcacc aaaccactaa agcccataaa ggccagagac ataagacctg cggtaattaa
    11941 cgcaatggca ttaccgcgaa aaggtgccgg gacatcagcc acagcaaggc gttcgcggat
    12001 ggcggcgaag agcaccatca ccagcgagaa accgacagcg gcggaaaaac cgtacagcgc
    12061 cgactgcaag aaattgtgcc cgagattgat attcagcaac gccacgccga gcactgcaca
    12121 gttggtggtg ataagcggca aaaaaatccc cagcagacgg taaagcaccg ggctggtttt
    12181 gcgcaccacc atctcggtga actgcacgac cacagcaatc accagaataa atgccagggt
    12241 gcgcaggtaa atcagattaa gtgggatcaa aatccacgta tcgataagcc aggcgcaaat
    12301 agacgtcagc gtcatcacaa acgttgttgc cagccccatg cccatcgcgg tttccagttt
    12361 tttggaaacc cccataaacg gacagagacc gagaaacttg accagtacaa agttattgac
    12421 cagtacagtt ccgacaaaca gtaacaggta gtcagtcatt tttcggcctg aaataaaaaa
    12481 gccgcctatt atccgttaat ccagagcagg cgacaacagg ttaactgtaa ggttattacg
    12541 gattcacaaa cgtcgctttt acccgcgacg agcgtttgaa ataggggacg atcaatgcgg
    12601 ttgccaacaa agtaaacagc aactggcgaa cggctatgcc aacttccacg ggtgaaaaag
    12661 cgaatgcttt tactgccagc aatacggaaa taagcagcca gataatatag tgtttaggaa
    12721 cacaacgccg acgtttaaag aaagcgatgg tcaaccacag tgtgtaatac cacatggcga
    12781 tagcggtaat aaaagaaacg ccccataaga tttgtgtcgt cagtgcttgt ccgccgagcg
    12841 tttgaaatgt ttgcggagaa gataacgcag cggtatacag caacaacgcc agcgtcgtac
    12901 tcaacagagc gactaaaagc caggccaacg ggccaagcaa ccagcctcca atacgttgtg
    12961 gcgttgtggt ggtcataggt tctccagaat agcgacgcaa acaggcaaat ctaattttag
    13021 gggcgttatt ataaggcttt tataaccgat cgctactttc tttattggac atagtgccag
    13081 acggacttcg gtacctggcc gaggtcaaac aaacgcccgc cagaaaccag ctctgcgcga
    13141 cggtgatcgg cagcacgata catattgatc agttcctgat cgtccgtcag ggtgtaatta
    13201 agatggtcgt agagtttttc cagactttcg agtgaactga ttttgcgaaa ttttaataag
    13261 tagtcctgaa cagtcataaa aggtccataa aataattaat accaaagggg taggtattat
    13321 tagtgatgat aaaatttcac caaaagtgaa cgcttgtgtt attacaataa taagaatgcg
    13381 ctgatggcaa atattcaggg atgaaaaact gctctgaaaa gagtaacgcg ctttactatc
    13441 cctgacaatc accaacaaca tcgaacaaga taataaattc ctggtttaat atccgacaag
    13501 tgaaaacatg cacccggacg ggcagcatgt cgctccacaa gtgcagagct tacttgtgtt
    13561 gtaccgaagc actctgttca ggtggctgat agttgtcaat gtgactcgcc acgccaagaa
    13621 gaatgactga aacgacaaga acgatccaac ctgttaattc aataagacga ttcattacag
    13681 cccacatctc tcttgattga tccattaact tcagggggta aatgttactt agcaataata
    13741 gctccagcaa gatttttact gaggtttttg cgatatttag cttttgtcgt tggaaaattc
    13801 gctatttttt tgacttaagt taaacaacat cccttattgc tggcaggtta ttaaactgtt
    13861 gagcgtgggt aaaggatagt gtcaaatagc catcatactt caatgagagg caatgacatg
    13921 agcgacaaca tccgtgttgg gttgattggg tatggttatg cgagcaaaac cttccatgcg
    13981 cccctgattg cgggcacgcc cgggctggaa ctggcggtaa tctccagcag tgatgaaaca
    14041 aaagtaaaag ccgactggcc aacggttacg gttgtctctg agccgaagca tctgtttaac
    14101 gatcccaaca tagacctgat tgtcattcct acacccaacg atacccattt cccgttagcc
    14161 aaagcggcgc ttgaggcggg taaacatgtg gtcgttgata aaccctttac cgtgacactg
    14221 tcacaagcgc gagagctgga tgcgctggca aaaagcctgg ggcgtgtgct gtctgtattc
    14281 cataaccgcc gttgggatag cgatttcttg acgctaaaag gtttgctcgc ggaaggcgtg
    14341 ctgggtgaag ttgcttactt tgagtctcat tttgaccgct tccgtccgca ggtgcgcgat
    14401 cgttggcgtg aacagggcgg cccaggcagc ggtatctggt acgatttagc accgcatctt
    14461 cttgatcagg ccattacgct atttggttta ccggtcagca tgacggtaga tttggcacag
    14521 ttacggcccg gagcgcagtc gaccgattat ttccacgcca tcttgtccta tccacagcgg
    14581 cgagtcattt tacacggtac catgctggca gccgctgagt cagcacggta tatcgtgcat
    14641 ggatcccgag gcagttatgt gaaatatggc ctcgatccac aggaagaacg tctgaaaaat
    14701 ggcgagcgtc tgccgcagga agactggggc tacgatatgc gtgatggcgt acttacccgc
    14761 gtggaaggtg aggaacgtgt cgaagaaacg ctgttgacgg tgcctgggaa ttatccggct
    14821 tactatgcgg cgattcgtga tgcgttaaat ggcgatggtg aaaatccggt tccggcaagc
    14881 caggcaatcc aggtaatgga gttgattgag ctgggcatcg aatccgccaa acatcgcgcg
    14941 acgttgtgcc ttgcgtgaaa aatggatggt gcaatttgca ccatccattt ttgttacttc
    15001 gcggcgactt tttctcgcag tgcgcgtttt tcctctgcgc tgaggaaagc catttccaga
    15061 ccattaatct gtgcctggcg gatttgctca cgggataacc cagcagctgg cgcggcaacg
    15121 gtatattcgt gaatgatatc cactccctgt acgccgggat cgtcagtgtt aatgctggca
    15181 cgaatgccat gctcaaggaa cgttttcagc ggatgtgcag ccagctctgc tacggtgctg
    15241 gtctgaatat tggaggtcag acaggattca ataccaattt gttgctcggc gagaaaatcc
    15301 atcagcgccc gatcttcaat ggcttttacg ccatgtccaa tacgctccgc acccagttca
    15361 cgaatcgcct gccagatgct ttccggcccg gcagcttcgc ctgcatggac ggtaatatgc
    15421 cagcccgcat cacgcgcgcg gttgaagtga gaaaggaaca gacttcccgg gaatccaagt
    15481 tcatcaccag ctaaatcaag tgcggtaatc tggtcacggt gggctaaaaa ggcctccagc
    15541 tcttgctgac aggcggcttc gccgaaggtt cggctcataa tgccgataag cttcgcctgc
    15601 acaccaaagg tgcggcaacc ttcacgtacg ccatcgatca ccgcttcgac aacacccgct
    15661 acaggcagct tatgtgccat tgccatgtag cctggtgaaa aacgcagctc gacatagcgc
    15721 aggccgttac gggctgcatc ttcaatgttt tcaaatgcca cgcggcggca ggcatcaaga
    15781 gaggcgagaa ctttaacgcc ccagtcaagt ttggtcagaa agctcaccag atcgggttcg
    15841 ttggcaatga cctgaacgtg gggaatcagt gtttcaaggg attgtgcagg aagcgagata
    15901 ttatactggc ggccaagttc aagaatggtc tggggacgaa tgttgccatc aaggtggcga
    15961 tggatatcag ttaatggcag ggtggtatca atcatggtcg cactcttttt ctggatataa
    16021 aagtgcgcac cattataaaa acaaagcagg gtaaaaatcc acgattgcgc aacgattttt
    16081 tacgttgcgc aattggggtt agcgaacagc gcggatggcg tttattaatc cagccacacc
    16141 tttttccagt ttcgaacgtg ggcagccggc attgagacgg acaaaaccac gaccttcttc
    16201 accataggta taccccggca tgatcgcgac tttttcttgc tcgataagcg ctttttgcaa
    16261 cgcgttgtcg tcaatattca acggacgtag atcaagccag gccagataag tggattgcgg
    16321 gatctgccag ttgagttcag gaaacgcggc gttcatttta tctgagatat acgtcaggtt
    16381 atctttcaga tagacgcgta aggcatccag ccacggtgca ccttgctgat aggcggcgat
    16441 atgggcagtt aacgccagta ccgaagggga agaaagccca tcacgtcctt tcagtgccga
    16501 taaataggca tcacggctgc tgctattttc tataatcccg taagcaccgg tcagcgcggg
    16561 aatattgaaa cttttcgatc ccgatgtcag caacgcccag tctccccgcg ccacattact
    16621 ccagggaata tgcggctgct cgccccagac catatccata tggatttcat cggaaataac
    16681 ccgcacatca tgacgctcgc acaggtcagc catgatctcc agctcatcgc acgtccacac
    16741 tttcccggta ggattctgtg ggttacacag gagcataatt ttacattctg gtttcgccaa
    16801 aacggcttcc agcttgccca tatcgcaaaa ccagccgtca gcctgcttct ctaaagcaac
    16861 gggcattact gtgcgctggt taccttcaat ggccttgtaa aatgcgtcat aggcgggcgt
    16921 gtggatcacc acgccttcac ctgtttcaga ccactgacga atcagttctg aaaccatata
    16981 gatgacagaa gggccataca ccaccgtctg cgtatcaatt gcggtgtaat gctgggtgga
    17041 aaaccagtgg gcaatagccg cgagaaactc atcgtttttc cagcggctgt agccaaatac
    17101 gccgtgcatc aggcgctgat tcagcgcctc gataatgcag ggggcagtgg caaaatccat
    17161 gtctgaaatc gtgaacggta acaggtcagc agtgccgaaa cggtcagcga cataatccca
    17221 ctgtgtacac catgtgccat gacgatccac gacctttgaa aaatcgaaca tatcttatcc
    17281 ttatgcctgg acagtatgca tcagaccggc catttcatct tttactgact gaacttgtgg
    17341 cccgataaca acctgcaggt tatgttgatt aagttgtact acgccaattg cccgattgtc
    17401 cttcagtgcc tgcacattaa caagcgacat atcttccaca gacaaacgca gacgggtaat
    17461 gcagttatcg aggctgacaa tattgtcggc accgcctaat gcttcgagga ttgcaggaac
    17521 gttgtaacct gatttacccg gcgcaccggc aacggctttt tcgattgagc tggcaacttc
    17581 gctatcgcgc cccggggttt tcagattgaa gcgggtgata gcgaaacgga agatgacgta
    17641 gtaaacgaca aaccagattg ccgccaccac tggcaccatg taccacttgg ttgacagacc
    17701 atgcaaaata ccgaacacca cgaagtcgat gatattgccg tcggtattac cgatggtgac
    17761 gccgagcaca gacatgacgg tgaagccgag gccggttaac agcgcgtgga tgacatacag
    17821 aactggcgct acgaacagga acaggaattc cagcggttcg gtagtgccgc caacgacgca
    17881 ggcgatcagg ccagaaatca gcagaccttt aattttatgg cgattttccg ggcgcgcgca
    17941 gtgatacata gctaacgctg cacctggtag accgccgaga aacgcaggca ttttaccttg
    18001 cgaaaggaaa cgcgtgacgc tttcagaaaa accgtgagtg gtcgggcaac tcaattgcgc
    18061 ctggaagatg gtcagtgcgc cgctgacggt ttgaccgcag acctcctgcg tgccgcctgc
    18121 gtcggtaaag cgaattaatg ccaccagaat gtgatgcaga ccaaacggca acagcagacg
    18181 ttcaccggta ccaaacagca tcggtccgaa atcacccgcg ctgtttatca tatggcccaa
    18241 gccgctaata cccatggcga aaatcggcca gactaatgga atcaccaggc cgacaaggcc
    18301 catcaccagc gaggagataa ttggtacgaa gcgcgtaccg ccgaagaatg ccagcgcatc
    18361 cggcaggcgg atattatgga aacgctcatg cagcatccag acgataatac cggcgatcac
    18421 cgcaccgagg atcccggtat cgatcgactg gatcccaaga atgctctgga tgttattggc
    18481 tttcagaacg gcggcatctg tggttggcag aatgccttta ttggtcaacc agaagtttac
    18541 cgcgaggttc attaccgcat aaccgacgaa gccagcgaat gccgctacgc ctttattttc
    18601 gcgtgccagg cccagcggga tggcgataca gaacatgaca ggcaggaaac taaaagcaaa
    18661 cgagccaatc ttactcatcc aggtaaagat agcctgcaac acggggttgc ccaggaccgg
    18721 gatcagggtt atgacatcat ggctgctaag agaactacca atgccgagca taatgccgca
    18781 gaacgacaat aatgccacgg gtaacatgaa ggttttgcct aactgctgga agaactccca
    18841 cagcgtgact ttcggtgctg ttttcgccgt cataaaacga ctcctcgtag agaacaatct
    18901 ggattcagta aattgcgcga gaagataaaa cgttttatca aattttagtg agtcataaat
    18961 cacattacgc aacgataata gcgggtataa gataaataaa aggtaaaacg ttttatctgt
    19021 cacataatca gggagtaggt catctgcatg gctaccgcca aaaaaataac cattcatgat
    19081 gttgcgctgg ctgcgggcgt gtcggtaagt accgtttcgc tggtgcttaa tggcaaaggg
    19141 cgaatctcta ccgccacagg agaacgcgtt aacgccgcca ttgaagagct gggatttgtg
    19201 cgcaatcgcc aggcgtcggc gctgcgcggc gggcaaagcg gcgtcattgg tttgatcgtc
    19261 cgtgatttat ctgcgccgtt ttacgccgaa ttgacggccg gattgacgga agctctggaa
    19321 gcacagggac ggatggtttt tttgcttcac ggcggtaaag acggcgagca gctggcacag
    19381 cggttttcac tgttactgaa tcagggtgtc gatggtgtgg taattgccgg ggctgcagga
    19441 agcagcgatg acctgcggcg gatggcagaa gaaaaagcta tcccggtgat tttcgcttcc
    19501 cgtgccagtt atcttgatga tgttgatacg gttcgcccgg acaacatgca ggctgcacag
    19561 ttgttgacgg agcatctcat tcgcaatggg catcagcgga tcgcctggct gggagggcaa
    19621 agttcctcat taacccgtgc agaacgggtg gggggctatt gtgcaactct actaaaattt
    19681 ggcctgccgt ttcacagcga ttgggtgttg gagtgcactt ccagccagaa gcaagccgcg
    19741 gaagctatca cggcgctttt acgtcataac ccgaccatca gtgccgtggt ttgctataac
    19801 gaaactattg cgatgggggc atggtttggt ttgctgaaag cagggcggca aagcggggaa
    19861 agcggagtcg atcgttactt tgagcaacag gtttcgctgg cggcatttac cgatgcgaca
    19921 ccaaccacac ttgatgatat acctgttacc tgggccagca cgccagcgcg ggaacttggt
    19981 atcacacttg cggatcgcat gatgcaaaaa atcacccatg aagagacgca ttcacgcaat
    20041 cttattattc ccgcccggct cattgcggcg aaataatcct ctctttatct gctatacttg
    20101 gtagtgtcct ttcctcaagg ttaatgataa aaaatgtggc acacaggcgg tgccttttat
    20161 ttttactgaa gttcacagga ggtttatgtg tttaattctg acaacctgag actcgacgga
    20221 aaatgcgcca tcatcacagg tgcgggtgca ggtattggta aagaaatcgc cattacattc
    20281 gcgacagctg gcgcatctgt ggtggtcagt gatattaacg ccgacgcagc taaccatgtt
    20341 gtagacgaaa ttcaacaact ggatggtcag gcatttgcct ggcgttgtga tattacttcc
    20401 gaacaggaac tctctgcact ggcagacttt gccgtcagta atctgggtaa agttgatatc
    20461 ctggttaaca acgccggtgg cggtggtcct aaaccgtttg atatgccaat ggcagatttt
    20521 cgccgtgctt atgaactgaa tgtgttttct tttttccatc tgtcacaact tgttgcgcca
    20581 gaaatggaaa aaaatggagg tggcgttatt ctgaccatca cttctatggc ggcagaaaat
    20641 aaaaatataa acatgacttc ctatgcatca tctaaagctg cggccagtca tctggtcaga
    20701 aatatggcat ttgacctggg tgaaaaaaat attcgggtga atagcattgc gccgggggca
    20761 atattaaccg atgccctgaa atccgttatt acaccaga
//
LOCUS       DAEBXN010000072        20779 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.072, whole genome shotgun sequence.
ACCESSION   DAEBXN010000072 DAEBXN010000000
VERSION     DAEBXN010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20779
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.072"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..579
                     /locus_tag="KJL99_002681"
     CDS             <1..579
                     /locus_tag="KJL99_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBD4437362.1"
                     /translation="GYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGVLNAM
                     ASQRAKEARGGELQINELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATKML
                     FSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQLVS
                     LPDFSHYELYDKPEAVQEALAKVIPFFNTHLG"
     gene            597..1235
                     /locus_tag="KJL99_002682"
     CDS             597..1235
                     /locus_tag="KJL99_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBD4437363.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFVEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            1526..2254
                     /gene="mpaA"
                     /locus_tag="KJL99_002683"
     CDS             1526..2254
                     /gene="mpaA"
                     /locus_tag="KJL99_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HBD4437364.1"
                     /translation="MTVTRSRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHNELGEWLAQSFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="KJL99_002684"
     CDS             complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="KJL99_002684"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HBD4437365.1"
                     /translation="MRIVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            3313..3819
                     /gene="tpx"
                     /locus_tag="KJL99_002685"
     CDS             3313..3819
                     /gene="tpx"
                     /locus_tag="KJL99_002685"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HBD4437366.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="KJL99_002686"
     CDS             complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="KJL99_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HBD4437367.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(5552..6613)
                     /locus_tag="KJL99_002687"
     CDS             complement(5552..6613)
                     /locus_tag="KJL99_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HBD4437368.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTVRLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(6610..8007)
                     /locus_tag="KJL99_002688"
     CDS             complement(6610..8007)
                     /locus_tag="KJL99_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HBD4437369.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDALLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAVWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWDESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AAMKADHVTVDQHANMVSLLQQLIQDAWQNAAFERISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGALLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            8163..9161
                     /locus_tag="KJL99_002689"
     CDS             8163..9161
                     /locus_tag="KJL99_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBD4437370.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(9272..10144)
                     /gene="ompG"
                     /locus_tag="KJL99_002690"
                     /pseudo
     CDS             complement(9272..10144)
                     /gene="ompG"
                     /locus_tag="KJL99_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006175591.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
     gene            complement(10217..11298)
                     /locus_tag="KJL99_002691"
                     /pseudo
     CDS             complement(10217..11298)
                     /locus_tag="KJL99_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(11312..11971)
                     /gene="pgmB"
                     /locus_tag="KJL99_002692"
     CDS             complement(11312..11971)
                     /gene="pgmB"
                     /locus_tag="KJL99_002692"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBD4437371.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(11968..14235)
                     /gene="ycjT"
                     /locus_tag="KJL99_002693"
     CDS             complement(11968..14235)
                     /gene="ycjT"
                     /locus_tag="KJL99_002693"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HBD4437372.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGAGYTIASRRFVSADQLPLIALEITIMPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTS
                     TQLHAGETVTLQKLVWIDWRDDRQAALDEWGSVSLRQLEMCAQQSYDQHLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHGERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMVYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMTKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSSASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LLEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLDGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(14232..14855)
                     /locus_tag="KJL99_002694"
     CDS             complement(14232..14855)
                     /locus_tag="KJL99_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBD4437373.1"
                     /translation="MTNGVLGEIYVTTARALRRCGVPGWGVFTNKELQGGGLLIDIGI
                     HMLDAAMYVLGFPAVKSVNAHSFQKIGTQKSCGQFGEWDPATYSVEDSLFGTIEFHNG
                     GILWLETSFALNIREQLIMNVSFCGDKAGATLFPAHIYTDNNGELMTLMQREMADDNR
                     HLRSMEAFINHVQGKPVMIADAEQGYIIQQLVAALYQSAETGTRVEL"
     gene            complement(14852..15274)
                     /locus_tag="KJL99_002695"
     CDS             complement(14852..15274)
                     /locus_tag="KJL99_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBD4437374.1"
                     /translation="MTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSHLSQA
                     QALAEKYGNASVWDDPQAMLLAVKPDVVSVGSPNRFHYEHTLMALEAGCHVMCEKPPA
                     MTTEQAWEMCDTARKLGKVLAYDFHHRLRSIRNSCVNR"
     gene            complement(15296..16071)
                     /locus_tag="KJL99_002696"
                     /pseudo
     CDS             complement(15296..16071)
                     /locus_tag="KJL99_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
     gene            complement(16089..17141)
                     /locus_tag="KJL99_002697"
     CDS             complement(16089..17141)
                     /locus_tag="KJL99_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HBD4437375.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(17172..18014)
                     /locus_tag="KJL99_002698"
     CDS             complement(17172..18014)
                     /locus_tag="KJL99_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBD4437376.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALLERFIKSGLTAG
                     GVKG"
     gene            complement(18001..18882)
                     /locus_tag="KJL99_002699"
     CDS             complement(18001..18882)
                     /locus_tag="KJL99_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBD4437377.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(18903..20186)
                     /locus_tag="KJL99_002700"
                     /pseudo
     CDS             complement(18903..20186)
                     /locus_tag="KJL99_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            complement(20200..>20779)
                     /gene="ycjM"
                     /locus_tag="KJL99_002701"
     CDS             complement(20200..>20779)
                     /gene="ycjM"
                     /locus_tag="KJL99_002701"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HBD4437378.1"
                     /translation="VNWKNNPDGTRSPYEMNVTYMNALSRRESSDEERCTRFILAHAI
                     LLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKYHSEEITRELNDEATLRHAV
                     YHELSRLITLRRSHNEFHPDNNFTIDTVNSSVMRIQRSNADGNCLTGLFNVSKNIQHV
                     NITDLHGRDLISEVDILGNEITLRPWQVMWIK"
BASE COUNT         5071 a         5668 c         5052 g         4988 t
ORIGIN      
        1 ggctatgcca ttaatgccgc gctgacagaa aaacgaatta aagctgtagt gagtattacg
       61 ggtgttaata tcgggcgtct ttttcgggaa ggtttcagca attatgatcc gattggcgta
      121 ttgaatgcaa tggcgtcaca acgagcaaaa gaggctcgtg gtggagaatt acagatcaac
      181 gaattacttc ccgccagcct ggatgcagca aaagcccatg gactgaccga acgcgatgtt
      241 tatgaagcaa ctgactacta taaaacacca cgcgggcaac aaccgggagg tgcgacaaaa
      301 atgttgttct cacatgcaca gaaaacgctg gcctgggatg ccttcgcatt tactgaagtg
      361 ttactgaccc agccggtgat ggtagtagtt ggtgaaaaag tcggtgcctt cggtgcttat
      421 cgtgatggac tggaagtcta tggccgggcg atggtttcgc aggatcgtca gttagtttcg
      481 ttacccgatt tctcacatta tgaactatat gacaaaccgg aagcagttca ggaggcgctg
      541 gcaaaagtta ttcctttttt caacacccat ctgggataag tttcatgaga aatattatga
      601 aaaatgtact gattcttggt gctggcggtc agatagcccg ccatgtgatt aatcaactgg
      661 cagataaaca aactattaaa caaactttat ttgcccgtca gccagcaaaa atacacaaac
      721 cctatccaac taatagccag attattatgg gggatgtact gaatcatgca gcactgaagc
      781 aggccatgca gggacaggat attgtgtatg ccaaccttac gggggaagat ttagatattc
      841 aggcaaattc agtgatcgct gcgatgaaag cctgtgatgt taaacgtctg atctttgttc
      901 tttccctcgg tatttatgat gaagttccag gaaaatttgt cgaatggaat aatgcagtta
      961 ttggtgaacc cctaaaacca ttccgcaggg ctgcggatgc aattgaggct tctggactgg
     1021 aatatacaat tcttcgccct gcctggctga ctgatgaaga tattattgat tatgagctta
     1081 cctctcgcaa tgaaccattc aaaggtacga tcgtatcacg taagagtgtc gcagcattaa
     1141 ttacagatat catcgataaa cctgagaaac acattggtga gaatataggc atcaatcaac
     1201 ccgggactga tggcgataag ccattcttca tgtaaatcaa ttatatagat accatcgtac
     1261 tgtactatgt gcagtacgat ttattaaact tattaaggtg aactgatcaa tcagatgcga
     1321 ttaacatgtg tagaaccagt aagaataaaa tacgccatgt atataacatt acgacaattc
     1381 taatatctgc acatcaatga aaatgcaaac aacttccggc aaaaaagtga tacaagatcg
     1441 atgacatagc gttgaagaaa aggatttgat aatccgcaaa atttgcacaa cactgcctgt
     1501 aatacttcct gaaccagaga cgatcatgac cgtaacccgc tcacgcgccg aacgcggcgc
     1561 atttccgccc ggaacagaac attacggacg ttcattattg ggcgcgccgt taatctggtt
     1621 tcctgcccct gcagccagcc gcgaaagtgg tttgattctg gctggcactc acggtgatga
     1681 aaactcttcg gtcgtcacgc tctcctgtgc tctgcggact ttgacacctt ctttgcgccg
     1741 ccatcatgtg gtgctgtgtg tgaatcctga cggctgccag ctggggttac gggccaatgc
     1801 taatggtgtg gatttaaaca gaaactttcc ggcggcgaac tggaaggaag gtgaaacggt
     1861 ttatcgctgg aacagcgccg ctgaagaacg tgatgtcgta ttactgacag gtgacaaacc
     1921 tggctccgaa cctgaaaccc aggcgctgtg ccagcttata caccgcattc aacctgcctg
     1981 ggtggtctca ttccacgatc cactggcttg tattgaagat cccagacaca acgaattagg
     2041 cgaatggctg gcccagtcgt ttgaattgcc tctggtaacc agcgtcggtt atgaaacgcc
     2101 tggctctttc ggcagttggt gtgcagatct caacttgcat tgcatcaccg cagagtttcc
     2161 gcccatctcc tccgacgaag ccagcgaaaa atatctcttt gcgatggcaa acttgctgcg
     2221 ctggcatcct aaagatgcaa ttcgcccgtc gtgaactgaa gcgccggttc cacatctacc
     2281 gccagccagg tcggtccgtc aagatcggcg aaactgacct gcggcaccag cggtaaagcg
     2341 gcgctaatgg cacgagaggt acacaacatg cagcccagca tcagactgaa accttgtgca
     2401 cgcgcttcag tcgccagcgc cagcgcttcc gtcagaccac cggttttatc gagcttaatg
     2461 ttaaccatct catagcgccc tttcagcgcc ttcagattgc tacgagtatg acaactttca
     2521 tcagcacaaa tcggcaacgg atgaataaaa ttctccagcg ccgcatcgtc ctgcgcagga
     2581 agcggttgtt caagcatcgc aacgcctaaa tccgccaata gctggcaacg cgccgccaac
     2641 ccttctgcac gccaggattc atttgcatca acgatcagcg tggcatcggg cacagcggtg
     2701 cgaattgcca ccatccgctc actgataaga tggttatcca gcttcacttt cagtaatttc
     2761 gcgcctgcct gccagagtgt tgatgcacta ttggccatct gatcaggcgt accgatgaca
     2821 accgtctgtg cagtgataac tgtctcggga agcgttatgc cgatcaaatc agccagcgat
     2881 tgctgctgtt ttcgcgccgc gagatcccac aatgcacaat ccagcgcatt acgtgctgcg
     2941 ccggcaggga gaattttttg caactcctcc cgtgtcagcc ctttctctaa ttgcggcacg
     3001 acgctcataa tttgcgccat taccgaggca tcactttctc cataacgcgg atacggcgtg
     3061 cattcgccgg tgcctttaat accctcttct tccagttcaa ccaccaccac gcgcgcttca
     3121 ctgcgacttc cccgggcaat cacaaacggg gtatgtaagg gccaggcttc ctcaaatacc
     3181 ttaacgattc tcatctttac tccttgttgt cgcggtggtc tgcggattaa gcgcaaatag
     3241 cgtttgctgt gttattgaca gttagcataa actaggtgtg acgttaacta tatgtaaaca
     3301 ggaaagataa tcatgtcaca aaccgttcat ttccagggca acccggttac agtcgccaat
     3361 tccatcccgc aggcgggtag caaagcgcag acttttactc tcgtggcaaa agatctgtct
     3421 gacgtcaccc tcggtcagtt tgcgggtaaa cgcaaagtgc tgaacatttt cccgagtatt
     3481 gataccggtg tttgcgccgc atcagtacgt aagtttaacc aactggcaac tgagatcgac
     3541 aacaccgttg tgctgtgtat ctctgccgat ctgccgttcg cccagtctcg tttctgcggc
     3601 gcagaaggtc tgaacaacgt tatcaccctc tccactttcc gtaacgctga atttctgcaa
     3661 gcctacggtg tggcaattgc tgatggccca ctgaaaggtc tggcagcgcg tgccgttgtg
     3721 gttattgacg aaaatgacaa tgtgattttc agccagctgg tggatgaaat caccaccgag
     3781 ccggattacg aagcagctct ggctgtactg aaagcataag ttaatatgcc tgatggtgtt
     3841 gcaccatcag gcatattcgc gcttactctt cgttcttctt ctgactcaga ccatattccc
     3901 gcaacttatt ggcaatcgcg gtatgtgaaa cgccgagacg ttttgccagt ttgcgcgtgc
     3961 tgggataatt gcgataaagc tgggttaata ccgagcgttc aaaacggctg gtgatttcgt
     4021 ccagcgaacc ttccatcgca tcttcgccca cggctaccgt tgcggcgtca taatccggca
     4081 acaaaatatc ctgtggacgc agctcataac cgtccagttg tgtcagtgcg cgatagatag
     4141 cgttctttaa ctgccgcaca tttcccggcc acgcataacg tgtaagtaca gtattcaggt
     4201 cagcggccag tttcggacgc ggcacgccct gctcgtcggc aaagcgggcg acgaacagct
     4261 cagttaacgg cataatgtcc tgcggacagt cacgtagcgg cggcagattg agcgtcaaca
     4321 cgttcagacg ataatagaga tcttcacgga acacgccttt ttgcaccagt tcgaccagat
     4381 tcttctgcgt agcgcaaatc acccgcacat cgacatgcac ctcatggtct tcgccaaccc
     4441 gacggaaagt gccatcatta agaaaacgca gtaatttcgc ctgcatccgt ggtgacattt
     4501 cccctatttc atccaacagc accgaaccac cgttcgcctg ctcaaagaac cctttcttcc
     4561 cttccggagc atgaccaaac agttcactct cgaccgcatc ttctggtata gacgcgcaat
     4621 tcagcgccag gtaaggtttg cccgctctgg ggcttgcctg atggcaggcg taggcaaaaa
     4681 gatctttacc tgtacctgtg tcacccgtaa tcagcagcgg cgcgcttagc atcgccagtt
     4741 tctgcgcctg ttcgacaacg tgcttcattt tcgggctgac ggcgacaatt tggctgaagg
     4801 cgctgacgtc ctgggcggcg acattttgca actggcggcc catacgaatc gttgatcgca
     4861 acatcaccac cgcaccggtc aggacgtgtt gatcattttc atcctgaaga taaacaggcg
     4921 taatctccat caggaaattc tgcccattaa taacgacatg ctcgttatgc gaatcttgcg
     4981 gttcgctttc cagccaacgt aaaaaattaa agccgttaat caattgtgcg gcggtatggt
     5041 tgcgcaggcg atccaatttt tgcccaaaaa gctgacagct cgccgggttc gccatatcca
     5101 ctttgctttt catatcgaca gagagcacag gttcaggcaa cgcctccagt aacgcgctca
     5161 acgccagatg ctcacgttcg gaaggcatcc acgggacagt acgcacatcg gtaacacctg
     5221 caatacggcg tatttcggcc atcagactgc tgaaactctc aaactccagt tcagcaaaat
     5281 tgaggtagat tcgcccaatg ggatcaatct caataccgcg taaatcaatg cctcttagca
     5341 cgagtagatc gagtaattcg cgggtcagac cgagtcggtc ttcacaaaag acttccagac
     5401 gcatgggaac cttcacctga aaaaagaaca attaatatga tgatatgaca ctatttggat
     5461 agcaggaagg tttctgtcaa caatcattga cacaaagacg gaaaaacggg cacttcggcg
     5521 taaagatatc cggtcagcac ccgatattgc attatttttc gctgggcgtt ttgccttttt
     5581 gcagagtttc tttcacctga ccgataagct gacgacggaa atccccaagg cgaggtttgt
     5641 catcgtcaat ccacggcagc gggcggcaaa gctccatagc tttaatcccg agtcgtaccg
     5701 tcagaagtcc agcaccaatt ccctgagctg cgcgggtaga caaacgagca gcgagatctt
     5761 gcgacatcca gtccatcccc acttcgcgca ccagttcgct ggctccggca aaagcgatat
     5821 tcagcaatac cagcttaaac agacgcaaac ggctgtaata ccccagttca atgccataca
     5881 gcgtggcaat gcgattaatt aaacgcagat tgcgccaggc gataaacgcc atatcgacca
     5941 acgccagcgg gctgaccgca atcatcaacg ttgattccgc cgccgaacgg ctgatttcgc
     6001 gccgcgcctg ggcatctaaa actggctgga ccaaatgcgc atacaggctg accacttcac
     6061 gatcgttctg cgtttcatgg attgaggcat accagcgttg cagcgccgga tgcgactgat
     6121 caatacccgc ctgctgcgcc agtttttcgc aaaatgcgcg acctttgccc gtgccgtggc
     6181 tatgcaataa atcacgcgct tcgtcgcgtt catgggcgcg ctgtcgcaag cgccataagc
     6241 gccgccactc tgttaccaca gaacctacgc cagcgccgat aatcaatgcc ccagcggcac
     6301 atccacccag cgctacccag tcctgggttt gccaggcatt cattgtccac tgtataccct
     6361 gcccgacaac gcttgcgcca aacagagcca gcccgcccat caccattttg cgccacaggc
     6421 tgcgtttcgg acgtaacgct gcgtccatta ccgcttcgac ttgcccctct tcttcctgcg
     6481 cttcgtcgag cgtggccggg gcaaaatttt gcgcctgatt ttcgtcaaag gtctgctgcg
     6541 ccctgaattt tggattctgt tcgacctcca gcggaccgtc gaaatcaata cgtggtttta
     6601 acggttcggt catcgcaatt tatctcctat taaaaattcc agcgcggcat caagacgaat
     6661 atgcggcagc ggtttgtcga catccatcac ctgcgggcga aacgcttcaa actggaaccc
     6721 ttgcttgtcc cagaacgcct gaccgggcaa acgcgcagga acttcaccgg ggtaaacggt
     6781 gagaagcgca ccatcgctga gccggttacc gcgcaatgcc ggaattttct cgccgttgac
     6841 gtcaataatg ccgctggtgg tcgcctgcac cgaggccagc cccaggcaat ccatactaat
     6901 cctttcaaac gccgcatttt gccaggcatc ctgaatcagt tgttgcagta atgaaaccat
     6961 attggcgtgc tgatcgaccg ttacatggtc cgctttcatc gcggcaaaca gcagtttgtc
     7021 gataaccggc gagaataagc ggcgaaacag cgtgcgctgc ccgtagtgga aactttgcat
     7081 cagctgcgtc agtgccagac gcatatcatt aaatgcctgt ggcccactgt tgagaggttg
     7141 caggcaatcc accagcacaa tctggcggtc aaagcgcaga aaatgattct tatagaaccc
     7201 cttcaccact ttctcgcagt aataattaaa ccgctcgcgc agcattccgg cattggtgtg
     7261 cttatcggcc tgcgccagtt tggactcgtc ccaggcatcg acatccggcc acgggaagaa
     7321 ttgcagcgcg ggcgcacctg ccatatctcc cggcaagaca aagcgcccag gctgaataaa
     7381 gtgcagcccc tgctgtttac agtggtggag ataatcggtc cacacggcgg caatgtctgc
     7441 cagacggttt tcgtcggcag gcgctagcgg gtcaagtcct tcgcacatca ttcgccattt
     7501 cgccgaccat tcgccgcgct gaccattgag taagcccgtc atctggcgcg accagcttaa
     7561 gtaatcctgc gccagcatcg gcaggtcgag caaccattcg ccgggataat ccacaatttc
     7621 cagatacagc gtggaggtat ccttaaagtg gcgcagcagc gcatcgttcg atttaaaacg
     7681 taacgccagg cgaatttcac tgacgccgcg cgtaggcgtc ggccaggcgg gaggatcacc
     7741 atataactgc gccagtcctt catcataggt gaagcgcgga atgccaaagt cacgctgggg
     7801 aatgcgtttt acgcccagca agcgctcttc acgcaccgcg cttaacagcg gcaaacgtgc
     7861 tccggcatga atattgagca actgattaac catcgcggtg ataaacgctg ttttgccgct
     7921 gcggctaagt ccggttacag cgaggcgcag atgtctgtcg acaccccgat tcaccagcgc
     7981 attgagttca tttttaagtc gcttcattgc tgtcctgtgc tgctctgaag accagaaaaa
     8041 aaatcattcg taaggatatg aatgtggggg ccaggccatt gattatcaac tgcccgtgtt
     8101 gtcatcgacg ggcgaactcc ataagatgtg cagcattcac gttcggacgg agttcctgac
     8161 ctatgtcccc tactatttat gatattgcca gggttgcagg cgtatcaaaa tccaccgtat
     8221 cacgcgtgct gaataagcaa actaatatct ccccggaagc gcgcgaaaaa gtgttacggg
     8281 ccattgaaga attacagtat cagccaaaca agctggcccg cgcgctgacc tcttcgggtt
     8341 ttgatgccat tatggtgatt tctacccgtt cgaccaaaac tacggcgggt aatccgtttt
     8401 tctctgaagt tttgcatgcc atcaccgcca aagcggaaga agaaggtttc gacgtgatat
     8461 tacaaacgtc gcacaatccg gcagaagatc tgcaaaaatg cgaaagcaaa attaaacaga
     8521 aaatgattaa aggcattatt atgctcagtt cgccagcgga tgagtcattt tttgcccaac
     8581 tcgataaata cgatattccg gtggtggtaa ttggcaaagt tgaaggtcaa tatgcccatg
     8641 tttattctgt cgataccgat aattttggtg acagcattgc gttgaccgat gcattaattg
     8701 aaagtgggca tcaaaatatt gcctgcctgc atgctccgct tgatgtccat gtttcggttg
     8761 atcgcgttaa tggctataag caaagtctgt ccgcgcataa tattgcagtg cgtgataaat
     8821 ggattgttga cggcggttat acccatgaaa cagcactgca agcagcacgg caattattaa
     8881 gtcagtcacc gttgcctgag gcagtgtttg ccactgacag cctgaaatta atgagcattt
     8941 atcgtgcggc ggcagagaaa aatattgcta ttccgcagca gttagcggtg gtgggttata
     9001 gtaatgaaac gctgtcgttt attttaacgc ctgcaccagg cggcatcgat gttccgacgc
     9061 aggagttagg tcaacaaagc tgcgagttat tattccgctt aatttcggga aaaccgtcac
     9121 cacagaatat taccgttgcc acgcatatga cgctgaaata aataccagct accttgcctg
     9181 aatgccggat gcgatgctgg cgcatcttat ccggcctaca tttatagatc ggataaagta
     9241 ctcgcgccgc atccggcaat ttagcccatt atcagaacga gtaatttacg ccgacacctg
     9301 cataatggaa tttatcgctg tcgccttcgt cgtgatcctg ccactcaaac gcgtattcca
     9361 gcgaaacgga aagtccgttc tggaaatcat aaccgtaaaa taaacccaca cggttaaaat
     9421 catggccttc acgttcaata tcatcctgcc agtcccagtt actctagcga tccagcccaa
     9481 tgcgcgtata cggcgtcacc gagtggttgc cgagcgtcag cggcaaatag gcgcgaattt
     9541 cttgtgtgga aaactcaccg ttattgcggc tgtcgtccat attgaagccg cgctcgagat
     9601 aatagttcac tcgcaaagca accgtttcgt tgaaggtata ttgcagacct gtttccgttt
     9661 cgacacgggt atcagcgtaa ccggtcgtgt tcaaatcgtt ggcaaattta tacatcgaca
     9721 accaaccgtt gaaacgtaaa tcatcagtca gtttcacatc ccagtctggc gcgattttcc
     9781 agcgctgcat attcgccgtg tctttgcccg gttcatcaac gtagtgataa ccataattac
     9841 ggaaaccgcc ggtcaggccg aaactgaaat catcgctttc gaggaactga taatgcacct
     9901 ccagctccgg gcgatcaaac ctcgttccac gtttacccgc actataatct accggccctt
     9961 cctgataata ggccagagca attctccacg gcccgttggc ggcattaaaa tagactgaag
    10021 gctccgccag cccatccata tcttcgccat aaccctcgac gttttctatt tcgtacatcg
    10081 caccgatatt aaagtgccag tcgctctttt cctcggcctg tgcgcaggcc attcccgcac
    10141 acatcaccag tgcggtacag ggtaataact ttttttgttc cttgtattat cctgggggaa
    10201 tgccttcccc ctgtatttag cgaattgcta tttccgtttc tgcatcaaag aaatgacatt
    10261 tcgtcatatc aaaatgaata gtgatatttt ctcctgcatg ataatcattt aacgcaccag
    10321 cacggaccac taactcgtgc ccccaaccgt ggtgtagagc ataaattccg caccggttaa
    10381 ttctgccacg ctaattttgg cggaaatgtt attttcctct tgcgcgtccg gatgaatatc
    10441 ttccgggcgt attcccatca cgatgggctt atgcaaactt tcctgtgttt tcagaaccgc
    10501 taatttctct tcgagaatgg ttaatttaag cgtttccgta acgaatttat cgccatcgat
    10561 cgtgccgcga ataaaattca tcgctggtga tccaataaat ccggcaacaa acatattcgc
    10621 gggttggtta taaacggttt tcggcgcacc tacttgctga acaatcccgt ctttcataat
    10681 cacaatccgc gccgccatgg tcatcgcttc ggtctgatcg tgggtcacgt agatcatggt
    10741 ggtgttcagt ttctgatgca gcttgctgat ctctgcacgc atttgtacgc gcagcttggc
    10801 atcaaggtta gagagcggtt catccattaa aaacacgccc gcttcgcgca caatcgcccg
    10861 cccgagcgcc acacgctggc gttgcccgcc ggaaagcgcg cccggcttgc gtttcaggta
    10921 ctcacgcagg ccgagaattt gcgccgccca gttcacccgc tcatcaatca cctctttggc
    10981 gattttttgc atcttcaggc caaacgccat gttgtcgtaa accgtcatat gcggatacaa
    11041 cgcgtagttc tggaacacca tcgcgatatt gcgtgctttg gctggaacgt cattcattcg
    11101 tttgccgtcg atcaacagat cgccgccgct gatctcctca agcccggcaa tcatgcgcag
    11161 ggtggtcgat ttaccgcagc ccgacgggcc gacaaatacg atgaactctt tatcggcaat
    11221 ttccaggttg aagtccttca ccacatgcac ctggttatcg tagatttttt gaatatgttg
    11281 taacgaaagc tgagccatgt tgattccttt gctatacgtt ttgccagaag gccgataacc
    11341 gcgaccaggt cagggagtcc gttgagggca acagtaattg cgccccggtt aagcccgcac
    11401 cgatccccac cgagcgcata ccgctggcgt taatggcgtc aatgcccgcc tgcgcatctt
    11461 caatgccgat acatgcctgc ggcggcacgc ccagcccggc acaggcagcg agaaagattt
    11521 ccggatccgg tttcgagttt ttaagttggg aagcatccgc gcagaaggtg aaaaactcac
    11581 gcagctccag cgccgctaaa atcgtcggcg cattcaggga gacggaagcc agccctaccg
    11641 gaatctgctg tgcacggagc tctgccagca aattgcgaat accgggcagc acagcattaa
    11701 ccgtcagctc gcgcagtgag tggacgtaaa gcagattttt gcgatacgcc agttgcgccc
    11761 gctcctgcgg gttaaagtcg ccctctttgc ccccgtgttg cagaatgcgc cgcagagact
    11821 catcgcggct gatccctttt agagattcgt taaactgcgc atcaatactg atgccaattt
    11881 cagcggcaat ctgctgccac gcctggaaat gcagatgcgc ggtatcggtg attacaccat
    11941 ccagatcgaa aattacccct tgcagtttca ttcatcctcc tgatgcgtgg tagcggtccc
    12001 attgaagggc aaaataaaat cacccaaaca gaaaacagat tcttcagcca cggatataag
    12061 ctgaccgtcc aaacgcagtg aaacgggcgc agaagttcga atcgcaatac gctgcgcgtc
    12121 gagagtgacc tgtaattcgc agccctgcca gaacagaggg aaagacaact gttgccactg
    12181 ctcaagtaac gccggattta gatgcaattc accgtcacgc acgctcaccc cggcaaaacc
    12241 ctgaatcgcc cccagccaga tagcgccagt tgcggcagca tggataccat catcacaact
    12301 atgcggatca gcaccaagat cgatttcagt cccctcgcgc cagaactgat aactttgggt
    12361 cagcaggcca cagcgtgcag caacaatgcc gtggattgct ttacttaatg acgagtcgtg
    12421 aatagtgcgc ggttcataaa attgcagatt ggcaagacac gatgccgatg agaactgctc
    12481 tggcagcatg taattgagca tcaccacatc agcttgtttg aggatctgca tctcgttcac
    12541 ttctgcgcgt gaataatcca gcagaatggt ttgcttcccc gccgccgctt tgtatttcgc
    12601 cagattaatc gccggcttag tcataaacga atcatcctgc ggcaaaacac cgtcgggctg
    12661 aatttctggc agccgcagtt ctttaaggaa catttcggca cgatgaataa acgcgtcgtc
    12721 actacagcca aactggcggg caatactcag cgcctgctgg acgttgtaat acaccatata
    12781 gctggtgaag gcgttattat tgacatgttc ggtatattcg tctggcccaa taacatcatg
    12841 aatttccaga cggtcgttaa ccctcaccgc gcggctaatc cagaactttg cagtttccag
    12901 aagtagcgcc atgccttcat gcgcaatgaa actttcatcc cccgtggtct gccagtattg
    12961 aataaccgcc caggcgatat cggccaccag atgatgttcc gcctgcgccg aggccacttt
    13021 ttgccgcagc ccggtgcgaa tgttaatggc ggcaaattcc ggcgtctctt cttcgccgct
    13081 gcgcgcgctt tcccacggaa atagcgcgcc ctgccagccg ttgcgtcgcg ctttctcctg
    13141 cgcgcctggc aagttgtgcc agcgataacg cagtaaactt cgggcaaccg tcggatcgct
    13201 aaacagatgg aacggcagca aaaatacttc tgtatcccag aaaacgtggc ctttgtagcc
    13261 ttcgccggtt aagccttttg ccgcaatact gctgcgctcg ccgtgagccg gcgtcatgat
    13321 gcgcagatga taaagcgcat aatctaacgc ttgctgatcg tgcgcatcgc cgccatttac
    13381 cgtgatacgg cgtttctgcc accattgacg ccagttttct gttgatgctg caagatgttg
    13441 gtcgtaactc tgttgcgcgc acatttcaag ctggcgaagc gacacgctgc cccactcgtc
    13501 taaagcagct tgcctgtcat cccgccagtc gatccacacc agtttttgca acgtcactgt
    13561 ctcgcctgca tgaagctgcg tactggtatg ttgcagtaaa cggcgctctt tggcggtata
    13621 gcattgctgc acatcaccgc tcacctggca gcaacaactg atggccacat cactgcgccc
    13681 atcctgggtg gtgtagatcc cctgcatcaa atgctgacca aacacccgca cctgggtttc
    13741 gtcgagatgc tggcgaccgt ggttggtttg cgtggcgtca atgcctgttg aaatcagcac
    13801 tgacgcgtcg gcgtccagtg gcataatagt gatttccagc gcaatgagcg gcagttggtc
    13861 tgccgaaaca aagcgacggc tggcgatggt gtaacctgcg ccgttgctgg tacgccagac
    13921 aacgctacgg cgtaattctc cactggcaaa gtcaagttca cgctgccagg cttcgcggga
    13981 taacgagaaa acctcaccat ttatggcaat ctccatcccc aagatatcag gcagattcac
    14041 cagttcgttg atttcacctt ttcccgcccg atgatacagc cccgccagat acatccctcg
    14101 cgtctggcgc gtgtaatctt cttcatggct ggcgcgaagc ccaagatagc cgttcccctg
    14161 cgccatcagc gatgcatact tgttcagggt atgctggctg aaatggggtt ctgataacgt
    14221 tactggcctg gtcataattc cacacgcgtc cctgtttctg cggattgata taacgccgcc
    14281 accagttgct ggatgatgta cccctgctcg gcgtcggcta tcatcacggg cttgccctgt
    14341 acgtgattga taaaggcttc catgctgcgc aaatggcggt tgtcgtctgc catttcccgt
    14401 tgcatcagcg tcattaattc accgttgtta tcggtgtaga tatgtgctgg aaacagcgtc
    14461 gcaccggctt tatcaccaca aaagctgacg ttcataatca actgttcgcg gatgttgagt
    14521 gcaaatgacg tttccagcca cagaatgccg ccgttatgaa attcaatggt gccaaacagc
    14581 gaatcttcga cgctgtaagt tgccggatcc cactcgccaa attgaccaca gctcttttgc
    14641 gtgccgatct tttgaaaact atgcgcattc acgcttttca ccgccggaaa gcccagcaca
    14701 tacatcgcag catccagcat atgaatgccg atgtcgatca gcaggccacc accctgcagt
    14761 tctttattgg taaagacacc ccagccggga acgccgcagc gacgcagggc gcgggcggtg
    14821 gtaacgtaaa tctctcccaa aacgccgttg gtcacctgtt cacgcagctg ttgcgtatcg
    14881 agcgcaaacg atggtgaaag tcgtaggcca gcaccttgcc cagtttgcgc gcggtatcgc
    14941 acatttccca cgcctgttct gtcgtcatgg cgggcggttt ttcgcacatc acatggcagc
    15001 ctgcttccag tgccatcagg gtatgttcgt aatgaaaacg gttaggtgag ccgacgctaa
    15061 ccacatcagg tttcaccgcc agcagcatgg cctgcggatc gtcccacacg gatgcattcc
    15121 cgtatttttc tgccagcgcc tgcgcctggg aaaggtggct gtcacagaca gccaccagtt
    15181 ccagatcgtt gcgggtgcag tagtacgaag catgaacctt atccgccacc tggcccgcgc
    15241 ctattatcgc gacccgcagc ggagagcttg tcattgcact tttcacaaag acctcttagc
    15301 aggtacgcaa ccaggccaac gaatcacggt acgcctgggc aggatcttct gcccggatac
    15361 gcccttcata caccacataa ccctgatagt tatcggcgcg cagctgttca aacagcgcgt
    15421 ggaaatcgag ggtgccactg cccggctggt aacgatggtt atcggcaata tgcacatgac
    15481 cgagcaagtc acggttgtcg tgcagcgcct gcgccaggtt atcttcttcg atgttcatgt
    15541 gatagaaatc gccgataatc tgcacatgtt taagattgtt ttcgacgatg taacgacgag
    15601 catcggcgag ggtgttgatc atatgatcct gatagcggtt taacggttcg agatacacca
    15661 cggttccggt acgcgcggcg acctgttcca gtacgcgcag ggaatcactc accagtttgc
    15721 ggtcgccgtc caggctacgc ggcgaggtca tcggcggtaa gcggaaggta aacatgcccc
    15781 acgctgccgg aaccccccca cttctgccag cgcctcgaga atgcgttcga tctgctttaa
    15841 gccattaaga cgacgctctt cgataaagtc accaatccag ccgtcatagc cgccgcaggc
    15901 ggtggtcact ggtaaaccgg tttctttgat cgccgctttg acttcttcga tgttgttgac
    15961 cagcagtttg ccgtcaatct caaaaccatc gaagcccatc tctttgatat aacgaaatct
    16021 ctcgcggata ttttccggaa agaatgcctg attctgtgtg ccgattttca ttttttcatt
    16081 cctttgaatt aaaacgtaac gcccattttg atgctctgtt ccggatgctg atcgacatac
    16141 tgcatgtagc tttccgggct gttggcaaag gtcactaccg ggtcgattaa atcttcgcaa
    16201 ttgagataac cgttcatcag cagttcccag caggtttctt caatacgctt acggctccag
    16261 cgcggataat ccgggttcgg ttcgctgcac gcgcgggaga aaacaatttt ggcgttattg
    16321 aaatgcgctt cgcgtccgag gttaaaacct tcagcaaatg gtttggcaaa cgcgacatag
    16381 gagatggtgc cgccgtaggc caggccgcgc agcgccgatt gcagcgcgtc ggcgtaaccg
    16441 ctggtttcga taatcacatc agccccctgc ttgccggtca gcgttttgat ctctttgcct
    16501 acgtcagtgc caatggggtt aaggcagaaa tcagcaccgt ggcgacgggc aatatcacag
    16561 cgatgggcga taggatcgac gccaattacc accgatgcgc cagcgcgttt cgccagttgg
    16621 atggcaattt gaccgatcgc gccaagccct accaccacaa caaaatcccc tacgcgtacg
    16681 ttggcatcgc gcacgccgct catggcaaac tgcgccgggt cgtaacagac ggcgtttttc
    16741 caggagctgc cttgcggcat tttgcgcagc ttgtagttat tcactgcgtt aatgatgacc
    16801 gtctcggaga gcgggccgta gccgcatacg ctgtcgccca ccgcgtagtc tgtaacgtcg
    16861 ctgccgcact cgataatgtc gccaaccacc atgttgccaa gctggaattt gccaaactca
    16921 atgccgcgcg gcgcatctgc cggacgcggg gtgaacatct gccactcgcc gttaaagtct
    16981 tcatcgataa acgggctggc ggcgcggaag tcgaccactt ccgttccgtg tttcggtgcg
    17041 ccgaaacgca cgcggatctt cacttcattg gctaaaatcg cccgatcttc atactcaacc
    17101 agcgcagcaa cacgcggtgc tgtggctact aactttttca tgtaaaactc cttgtaaaga
    17161 aactggccgc tttagccctt cacgccaccg gcggtcaaac cactcttaat aaaacgttcc
    17221 aaaagcgcat acataatcac caccggcaat gcggtcacca gcgaggccgc catcatccgt
    17281 ccccagatgt aatcaggcgt actgaacagc gcgttcaggc ccactggtaa ggtgaaattg
    17341 ctggcgctgg agaggaaaat cgacgcaaac agatagtcgt tccacgccac cataaagcag
    17401 tagacgaaca ccgatatcag cccggacatc gccaggggca cggtgatacg gaagatgatt
    17461 tgcagacggt tgaggccatc catcatcgcc gcttcttcga tctcatcagg gatggtgtcg
    17521 aagtaacttt tcagcataaa cacggcggtg ggcagggttt gcgtcaccat ggtaatgatc
    17581 agcgccatct cggtgtcgta aataccaagc gcggtgatga ttttgaacag aggcaccacc
    17641 agcaaaatgc cagagaacat atacaccgtg taaaagctgg cgttgatggt catccggcct
    17701 ttaaagcgca ggcgggaaag cgcgtaagct ccgagaatgc cgagaaatac cgccaccacg
    17761 gaggaaacca ccgacaccac catgctgtta cggaagtagt cgacaaacgg gaaaatcatc
    17821 gggttaaaaa tgtcgacgta atgctccagc gtccactgtt gcggcagcag cgtaggatgc
    17881 agcgatatcg cctctttcgc gcttttgaac gaggtcatca gcatcacaaa aaacggaaac
    17941 agcgtgatga tgagaaacag cgccagcccg cagtaaaaac cgatacgact gagcgtgcgt
    18001 ttatttgttg ccattgaggt tcacccgttt cctggtcagc agaatgacag cgaaaataat
    18061 gatgaagagc accaccgaga tcgccgccgc tttccctaag tcattaaagg caaaggcggt
    18121 tttgtagaga tagacaccaa gaatatcgac cttggttgtc agcagataaa catcagcgaa
    18181 catgtagaac atccagatgg tgcgcagggt caccaccgtc gccaggaccg gcataattgc
    18241 gggcagcgtg acgatgcgaa accgctgcca ggcattagcg ccatccatct ctgccgcttc
    18301 gtacagcgat ttgtcgatgg tctgcaaaat cgccagaaac gagataaagg catacgggaa
    18361 gtagcgccag atggcgaaca gcaccaccag cacaaagcta ctgcccggat tatcaaacca
    18421 cagcggtgcc tgctcataga gatgcagaag atcgacgccg aggtagttca caatgccgta
    18481 gccgttgttg aacatgtatt tccaggcaaa caccagcgaa atggacggcg ttacgtagga
    18541 gaggatcacc agcgaacgcg cggttttgcg caggcggaac tcgcggttga aaaacatcgc
    18601 cacggccagc ccgagcacgg tgctgcccgc caccaccagc gccgtatacc agaccgtcat
    18661 ccacagcgag tgccagaagc cgggatcgga gagaatgcgc acatagttac tcaccccaac
    18721 aaacgttgac tcgatgttgg gattgagcgg cagacgtaaa aaactgattt cgatattcga
    18781 caccatcggc cacgccacca gaccgcccag cagtaataag ctgggcgcga gaagcagcag
    18841 cgcaaacggc atatcggaac gacctgaaaa aagcctgttc attgcggatt cctgtcctgg
    18901 gtttagtgct gttcgatcag ttcatccagc tttttctggc tcgcttgcag cgtgccaggg
    18961 agatcggctt tacccacggt gacgttatgc accatggagc tcaccacacc agaacccgtc
    19021 acatcgccca tgcgggtaaa gtttttatcc cctactgcgc caaagacctg aatatttggc
    19081 agttcaccga ttagctgatt cggtagttca cccagcgcct taataacgtc gttgtctttc
    19141 caggtggcgg tagtgacaac cgctttattc accggcagcg cagcacctgg cgacatcatc
    19201 acccagtcgg caatgttgtc cgcctgctcc ataaaggtga caaatttttc tgctgcttcg
    19261 gtcttttgtc cggcggtaat ggtcagcgag gtcaacatgc cgtagaccgc agagtttttc
    19321 tcggttggca ccacgaaacc gacgtttttc gggtcgcctt ctttaatcac agccggaagg
    19381 atataggtgg agtaaatcgc catcggcgcg gtgccgttca taaaggcgtc tttcacttcc
    19441 atgatgtcgt tagaacccgg catggtgttg gcagcaaggt cgcggtaata ggtcagcgcc
    19501 tgcatcatct ctggtgtatc aagggtgatt ttgccttcgg cgttaaagac gttggcctag
    19561 ttggataacg caaactggga gaaggattgt tccgtcagca cgctttctgc cgtaggcagc
    19621 gcaataccgt attttttatt cgccgggtca ttcagtttct gtgcaacgtc cagcagctgt
    19681 tgccagtttt tcggctcctc cagccccgct tttgccagca catctttgcg ataccagatg
    19741 ccgccaatcc aggcgctgac aggaacaccg gtccatgcgc taccatcttc ggtacgcaca
    19801 atacgcagta cgccatcgta aaacgcgcct tcaccaacgt tgctgatgac cgtggcaacc
    19861 gctttgcgat cgataagctg ctctttatcc ataactttgg cgtagtcatg gctggtttcg
    19921 atcacttccg gcagcgagcc gctacgtgaa agggtgatga ctttggtgtt atgagcgtct
    19981 tcttccacgg gcacttgttt aacggtgata cctgggtttt ctttttcaaa acgggcaatc
    20041 aatttgctga taactgcctg acgctcctgc tccaccgaag aatgcataaa ttcgatggat
    20101 acatgggttt tattttcttc tttacagcct gaaatcaggg cgcaggaaac cagtgctgat
    20161 aacagcacga tttttgattt aatcatgaga tgttcctttt tatttaatcc acataacctg
    20221 ccaggggcgc agcgttattt cattacccaa tatatcaact tcactaatca gatcccgacc
    20281 atgcagatca gtaatattta catgctgaat atttttactg acattaaaca atccagtcag
    20341 acaattacca tcagcgttac ttctttgaat acgcattacg gatgaattaa ccgtatcgat
    20401 ggtaaaatta ttatccggat gaaactcgtt atggctgcga cgaagtgtaa ttaaacgcga
    20461 caactcatga tataccgcat gccttaatgt agcttcatcg ttcagttctc gtgttatctc
    20521 ttcactgtga tattttttac ggttaatcgc acggttatat ccgagttttt cgacacctgc
    20581 gtaatcatta cgcgagccaa gaatactttg aatatatatc gcgggtacac cggggaaact
    20641 taacaaaatc gcatgggcaa ggataaacct ggtgcaacgt tcttcatcgc tactctcgcg
    20701 gcggcttaac gcattcatat aggtgacatt catctcatag ggactgcgcg tgccgtcggg
    20761 attatttttc cagtttact
//
LOCUS       DAEBXN010000073        20691 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.073, whole genome shotgun sequence.
ACCESSION   DAEBXN010000073 DAEBXN010000000
VERSION     DAEBXN010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20691
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.073"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            81..350
                     /locus_tag="KJL99_002702"
     CDS             81..350
                     /locus_tag="KJL99_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HBD4437379.1"
                     /translation="MQTVKLRQQGGAMIVTIPRDLAIDLGWSPGTELTVEKKGDSVNL
                     RATEHKPRGRLTVAQLLSQIDESEITELNQSTEGWAEGKKGNEAW"
     gene            344..709
                     /locus_tag="KJL99_002703"
     CDS             344..709
                     /locus_tag="KJL99_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBD4437380.1"
                     /translation="MVKARTPHRGEIWYFNPDPVAGHELQGPHYCIVVTDKKLNNVLK
                     VAMCCPISTGANAARSTGVTVNVLPRDTQTGNLHGVVLCHQLKAVDLIARGAKFHTVA
                     DEKLISEVISKLVNLIDPQ"
     gene            complement(710..1498)
                     /gene="bioH"
                     /locus_tag="KJL99_002704"
     CDS             complement(710..1498)
                     /gene="bioH"
                     /locus_tag="KJL99_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HBD4437381.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRVLVVSES"
     gene            1536..2219
                     /gene="gntX"
                     /locus_tag="KJL99_002705"
     CDS             1536..2219
                     /gene="gntX"
                     /locus_tag="KJL99_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HBD4437382.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            2278..2853
                     /gene="nfuA"
                     /locus_tag="KJL99_002706"
     CDS             2278..2853
                     /gene="nfuA"
                     /locus_tag="KJL99_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HBD4437383.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            3214..4530
                     /gene="gntT"
                     /locus_tag="KJL99_002707"
     CDS             3214..4530
                     /gene="gntT"
                     /locus_tag="KJL99_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HBD4437384.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(4575..6659)
                     /gene="malQ"
                     /locus_tag="KJL99_002708"
     CDS             complement(4575..6659)
                     /gene="malQ"
                     /locus_tag="KJL99_002708"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HBD4437385.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWQLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYKNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(6669..9062)
                     /gene="malP"
                     /locus_tag="KJL99_002709"
     CDS             complement(6669..9062)
                     /gene="malP"
                     /locus_tag="KJL99_002709"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HBD4437386.1"
                     /translation="MSQPIFNDKQFQEVLSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEVPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVMKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLTDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VCLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENLQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            9674..12379
                     /gene="malT"
                     /locus_tag="KJL99_002710"
                     /pseudo
     CDS             9674..12379
                     /gene="malT"
                     /locus_tag="KJL99_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
     gene            complement(12422..13438)
                     /gene="rtcA"
                     /locus_tag="KJL99_002711"
     CDS             complement(12422..13438)
                     /gene="rtcA"
                     /locus_tag="KJL99_002711"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HBD4437387.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFSTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGIFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(13442..14668)
                     /gene="rtcB"
                     /locus_tag="KJL99_002712"
     CDS             complement(13442..14668)
                     /gene="rtcB"
                     /locus_tag="KJL99_002712"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HBD4437388.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQLFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            14849..16447
                     /gene="rtcR"
                     /locus_tag="KJL99_002713"
     CDS             14849..16447
                     /gene="rtcR"
                     /locus_tag="KJL99_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HBD4437389.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCIYQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSTPRKKEQPRGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(16429..17187)
                     /locus_tag="KJL99_002714"
     CDS             complement(16429..17187)
                     /locus_tag="KJL99_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HBD4437390.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(17204..18034)
                     /gene="glpG"
                     /locus_tag="KJL99_002715"
     CDS             complement(17204..18034)
                     /gene="glpG"
                     /locus_tag="KJL99_002715"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HBD4437391.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWLAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYMQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(18079..18405)
                     /gene="glpE"
                     /locus_tag="KJL99_002716"
     CDS             complement(18079..18405)
                     /gene="glpE"
                     /locus_tag="KJL99_002716"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HBD4437392.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            18595..20100
                     /gene="glpD"
                     /locus_tag="KJL99_002717"
     CDS             18595..20100
                     /gene="glpD"
                     /locus_tag="KJL99_002717"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HBD4437393.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYTRTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(20369..20599)
                     /locus_tag="KJL99_002718"
                     /pseudo
     CDS             complement(20369..20599)
                     /locus_tag="KJL99_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443257.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
BASE COUNT         4893 a         5694 c         5213 g         4891 t
ORIGIN      
        1 gtcagtagat actctaccct gcttaagtgc tactttgtgc tatcttgcat gtaacacaaa
       61 gtagcactta gggaactgat atgcagacag tgaaattacg gcaacaagga ggagccatga
      121 tcgttactat cccacgagat cttgccatcg atcttggctg gtcaccgggc acagagctta
      181 ccgtggagaa aaaaggcgat agcgttaatt tgcgcgccac agagcacaaa ccgcgtgggc
      241 gtcttaccgt cgcgcagttg ttgagccaaa tcgatgaaag cgaaattacg gagctaaatc
      301 agtccacaga aggatgggct gaagggaaaa aaggtaatga agcatggtaa aggcacggac
      361 gccacatcgt ggtgagatct ggtattttaa ccctgatccg gttgccgggc atgaacttca
      421 ggggccacat tattgcattg tggtaacgga caaaaaactc aacaatgttt taaaagttgc
      481 tatgtgctgc ccgatttcaa caggggcaaa tgcagcacgt tccacagggg tgacggtgaa
      541 cgtcctcccc cgtgatacgc aaaccggtaa cctgcatggc gttgtacttt gtcaccagct
      601 aaaagccgtc gatcttattg cccgtggcgc taaatttcat accgttgccg atgaaaaatt
      661 gattagtgaa gttatcagta aactggtgaa tttaatcgac ccacaataat tagctctcac
      721 ttaccaccaa caccctctgc ttcaacgcca ccagcaggtg acaaaactcg accggatgcg
      781 aaataaacgg cgcatgggcc gctttggcga agatatatga ttcgctgtga ggccaaagtt
      841 tatccagcat cggcaccact ttgcgcggca ccagaccgtc gagatagcca tacaatcgca
      901 aaaacggcat ggacacgttt tgcagcggct gacggagatc gaccgttttc aggatttcca
      961 gcccgccatt aagcacgtca acctccggca tcggtaacgc cagaacggtt ttcttcaacg
     1021 cccgcgcatc ctggcgcgcc gtttcagtcc ccatggtttg taacgccagg aaccgctcca
     1081 ctgtacgctg aaaatcatca ctgagttgct gctgaaatcc cgccagcacg tccggtttta
     1141 tccccggcca ctcgtcacga gcactaaaac aaggtgacga cgccacggtg accagcgcct
     1201 gaacacgctc gggatgggtt aacgcaatct ggcttgccac cagcccgccc agactccagc
     1261 ctaaccaaat ggctttatca ggtgcctgtt gcagcacggc ttcggccata tcagcaagtg
     1321 acagcgcacc aaatccccgg ctacgcccga agccgggcag gtcaacaagg tgcagcgtaa
     1381 aatgcgagct aagttcctcg tcaatgcaac gccacacttc ggcattcagt ccccatccgt
     1441 gcagcagcac aagatgaaca ttcccctgac ctttggtctg ccaccagatg ttattcatcc
     1501 gctattgttc tcttttgact tacaaggatg aacatatgct aacagtaccg ggattatgct
     1561 ggctatgccg aatgccactg gcgttaggtc attgggggat ttgttcggtc tgttcacgcg
     1621 ccacccgcac agataaaacg ttatgcccac aatgtggatt acccgccaca cactcccatc
     1681 ttccctgcgg tcgctgcctg caaaaaccgc cgccctggca aagactggtc acggttgccg
     1741 actatgcgcc gccgttaagt ccgcttatcc accagcttaa attttcccgg cgcagtgaaa
     1801 tcgccagcgc cctgtcacgt ctgttactat tggaagtctt acacgctcgt cgcaccaccg
     1861 ggttgcaatt gccggatcgc atcatcagcg ttccgttatg gcagcggcgt cactggcgtc
     1921 ggggatttaa tcagagcgat ttgctgtgtc agccgttatc acgctggttg cactgccaat
     1981 gggatagcga agccgtcaca cgtacacggg ccactgcgac ccagcatttt ctcagtgccc
     2041 ggctgcgcaa gcgcaacctg aaaaatgcct ttcgtcttga attgcccgtg caaggtcgcc
     2101 atatggtgat tgtggatgat gtcgttacca ccggaagtac cgtcgcagag attgcgcagt
     2161 tgcttttacg caatggtgcg gcgactgtcc aggtctggtg cctttgtcga accttgtaga
     2221 gcctcgatga tgggcgtatt ataaccaact aaaatagtca actattaggc cattactatg
     2281 atccgtattt ccgatgctgc acaagcgcac tttgccaaac tgctggcaaa tcaggaagaa
     2341 gggacacaaa tccgcgtatt tgtgattaac cctggcacgc ctaacgctga atgtggtgtt
     2401 tcttattgtc cgccggatgc cgtggaagcc accgacactg ccctgaaatt tgacctgctg
     2461 accgcgtatg ttgatgagtt aagcgcacca tacctggaag atgcagagat cgactttgtt
     2521 accgaccagt tgggttctca gttaacgctg aaagccccga acgccaaaat gcgtaaagtg
     2581 gcagacgatg caccgctgat ggagcgcgtg gagtatatgc tgcagtcgca gatcaaccca
     2641 cagcttgctg gtcacggtgg tcgcgtttcg ctgatggaaa tcaccgaaga cggttacgcc
     2701 attctgcaat ttggcggcgg ctgtaacggt tgttccatgg tcgatgtgac gctgaaagaa
     2761 gggatcgaga agcagctgct gaacgaattc ccggagctga aaggtgtgcg cgatctcacc
     2821 gaacaccagc gcggcgaaca ctcctactac taagttatcc cctcttttgt agattgcccg
     2881 atgcgacgct aaagcgtcat ttcgggccta cgttgaacat gtgccagacg ttggcacagc
     2941 attgctttgg caaatccctc ccttcgtttc cccgcgataa tatgaccaac ctctcataat
     3001 ttaaatttac cccgctctgg tgattctcaa acgccaaatg ttacccgcat cattcacatg
     3061 ggtaccaaac atactcctga catctgacta caataattag ttttagtggg tatcagtcgt
     3121 ggtgccgcaa tatctctgtt cccgattggg ataattagag tttgtcgtca gaaaattgac
     3181 gttacccata acaaatgaaa ggccaggtaa atcatgccat tagtcattgt tgctatcggt
     3241 gtaatcttgt tgttgctcct gatgatccgc ttcaaaatga acggcttcat cgctctcgtc
     3301 ctcgtggcgc ttgctgttgg attaatgcaa ggaatgccgc tggataaagt tattggctcc
     3361 atcaaagccg gtgtcggcgg gacgctcggt agccttgccc tgatcatggg ttttggcgca
     3421 atgctgggca aaatgctggc agactgcggt ggcgcacaac gtatcgccac cacgctgatt
     3481 gccaaatttg gtaaaaagca catccagtgg gcggtggtgt taaccggttt taccgttggt
     3541 tttgccctgt tctatgaagt gggcttcgtg ctgatgctgc cgctggtgtt taccatcgcg
     3601 gcttccgcga atattccatt gctgtatgtt ggtgtaccaa tggcggctgc actgtctgtg
     3661 acccacggct tcctgccacc gcatccgggc ccgactgcga ttgccaccat tttcaatgcc
     3721 gatatgggta aaaccctgct gtacggtact attctggcaa tcccgaccgt gattctcgcc
     3781 ggtccggttt acgctcgcgt gttgaaaggt atcgataagc caatcccgga aggtctctac
     3841 agcgcgaaaa ccttcagcga agaagagatg ccgagctttg gcgtcagcgt ctggacctct
     3901 ctggtgccgg ttgtgctgat ggcgatgcgt gcgattgccg aaatgatcct gccgaaaggt
     3961 cacgctttcc tgccggtagc ggagttcctc ggtgacccgg taatggcaac gctgattgcc
     4021 gtgctgattg cgatgttcac ctttggtctg aaccgtggtc gttcaatgga tcagattaac
     4081 gacacgctgg tttcttccat caaaatcatt gcgatgatgc tgttgatcat cggtggtggc
     4141 ggtgcgttca agcaggtgct ggtagacagc ggcgtggaca aatacattgc ttccatgatg
     4201 cacgaaacca acatttctcc gctgctgatg gcctggtcga ttgctgccgt actgcgtatc
     4261 gcgctgggtt ccgcaaccgt tgcggcaatc actgcgggtg gtatcgcggc accgctgatt
     4321 gcaacgacgg gtgttagccc agagctgatg gttattgcgg tgggttccgg tagtgtgatt
     4381 ttctctcatg tgaacgatcc gggcttctgg ctgttcaaag agtactttaa cctgactatc
     4441 ggcgagacca tcaaatcctg gtcgatgctg gaaaccatta tctcggtgtg cggtctggta
     4501 ggctgtctgc tgctgaatat ggtgatttga taacactgac tgccggatgc atcgcatccg
     4561 acatcaaccg cactctactt cttcttcgct gcagctctgc gccgtctgtc caaatccttc
     4621 agcaacttgt tcacgccatc atcggcaaac atcgactcaa gcgttgcgga aagcttgcgt
     4681 cgccagtttt tatactggta actggtgcca ggaatattca ccggttcggc catatccagc
     4741 cagtcttccg gctgtagtcc taacagagca ctgttactgt cggcaatgta gcgctgcaaa
     4801 ccacggttca gcgtcggcgt catcgacatc aacgatgcct tatgcccggc acgttttggc
     4861 agacaaccat atttatgcag tgcatccagc agcccttgct tcgccagttc gcgatcctga
     4921 tacagaccgc gcagtaccac ttcatccgga tacagcccca gggttttgcc cagcgttaga
     4981 tccccgctct cccagtaacc acgcagcgtt ggcaggtcat gtgtcgccgc aaccgccatc
     5041 gactgctccg gatacgcttt cggtgcacgg aacgtcttct catggtcgtt ttcgaaatag
     5101 agcactttgt aagagtacac accgctgctg cgcagcttac caacaatctc taccggtacg
     5161 gtaccgagat cttcaccaat caccatacag cgatgacgtt tactttcgag tgccagaatc
     5221 gagagcagat catccaccgg atagtgaaca tacgcgccct gatctgccgt ctcgccatac
     5281 ggtatccacc acaaacgcag catcgacatc acatggtcaa ttcgtaatgc gccgcagttt
     5341 tgcatattgg cacgcaacag ctcgataaac ggttcatagg cacgcgcggt gatgatatgc
     5401 gggtccattg gcggtaatcc ccagttctgc cccaacgggc cgaggatatc cggcggcgcg
     5461 ccaaccgatg ctttcaggca atatagttca cggtcacacc aagtttccgc cccaccttcc
     5521 gctacgccaa ccgccagatc acgatacaag ccaatcggca tttcatagcc ctggcttatc
     5581 tcccagcagg cggcaaactg gctgtaagcc agccactgca accagagata aaaatcgacg
     5641 tcatcacgat gttcttcgca gaactgacgc acttctggtg aatccacgtt cttatacatc
     5701 tctggccatg caggccagcc ccagcgcatt tcgtcctctt tcacttgctg ggcatgtagc
     5761 gcatcaaagg cagcctgcca gaacaggctg tcgccctgct ctgcaacaaa ctggcgaaac
     5821 gcggccatct gctcatcatc acgttgcgcg aaacctttcc acgccattcg taatgctgtc
     5881 atttttaggg cggtaaccgt ggagtaatcg acccagtcgg catcgcgcgc ctgttgcagc
     5941 gtctgttgcg tggtcggcaa ctgccaccag gcctgagcct cttcgctaag atggaaatct
     6001 tcaacggcgt taacgtcgat ataaatcaca ttcagccaac ggcgagaaga cgggctgtat
     6061 gggctggcgc tctccggatt tgccggatag agcgcatgaa tcgggttcag gccaatgaac
     6121 gacccgccac gttttgccac atccaccagc atcgctttga gatcgccaaa atccccaata
     6181 ccccagtttt tttccgatcg cagcgtataa agctgaacgc aggcacccca cagcttttgt
     6241 ttattcagca aagcctgcgg ttcgtaacaa cgtttcgggg cgacaatcac ccggcaatgc
     6301 gcgcgctggt cgtcctgggt gagtgttagc gtgtgataac cttccggcag cttcgtcggt
     6361 agattgaacg ctttgccccc cgttacatgg cctttgtact gcgttccttc ttcggtggtc
     6421 agctgccagc tatattcgcc gctgccctcc accaccatcg gcattttttt gccgctggta
     6481 taaaccatga cattcgggac tggcgttacc gccactttcg tggcggtacg ttgatgcatc
     6541 gcgtcaagca aacgccgttt ggtttcggcg ctaatcgact gcggtttacc gtgggcattg
     6601 atgtaattgg ggctaatccc cgccgccagc gcggcattat ccagacgttt gctttccatc
     6661 gagcttcctt agcgttttgc ctgccagata cgagcctgat aatcgcgaat agagcgatcc
     6721 gagctaaaca taccgcagcg ggcggtattg aggatcgccg cgcgagtcca ggcctcctgg
     6781 tcgcggtaca gcacatccac ctgcttttgt gcctcaacat aggctgcgaa atccgccatc
     6841 accagatacg gatcgccgcc ctgtttgccg atactgtgca gcatctggtc gaaggcatgc
     6901 ttatcgccgt cgctgtattt accgctttcc agctctttca atactgcgtc cagcacctta
     6961 tctttcttcc gccatttcac cgggtcgtag cctttggcca gaatagcctt cacttgttcc
     7021 acggtatgac caaaaataaa gatattttct tcaccgactt tctcggcgat ttcaacgttc
     7081 gcaccatcca gcgtaccgac agtaagcgca ccattgagcg ccagtttcat attgccggta
     7141 ccggaagctt ctttacctgc agtcgaaatt tgttcggaga tatccgccgc cgggatcagt
     7201 ttttccgccg ccgaaacgca ataatccggc aggaacacca ccttcaactt atcgccaacc
     7261 agtggatcgt tgttgatcac gtcagccact ttgttgatcg caaagataat attcttcgcc
     7321 aggtagtagc ccggtgccgc tttcgcgccg aagaggaaga cgcgcggtac gcgatcagcc
     7381 tgcaggtttt cacgaatttc tttgtacaac gccagaatat gcagcagatt caggtgctgg
     7441 cgtttgtact catgcagacg tttgatctga atatcgaaaa tcgcctgtgg attgatctca
     7501 ataccggtac gaactttcac aaactccgcc agacagactt tattcgcctg cttgatctcg
     7561 cgatattgct gacggaattt cgcatcatca gcgaattttt ccagattgat cagctgatcg
     7621 agatcgttag cccactcttt ttgcagtgat ttatccaaca gagccgccag tgcagggttg
     7681 cactgtttga tccagcgacg tggggtaata ccgttggtaa cgttatggaa tttgttcggc
     7741 catagctggt gatattccgg gaacagatct ttcaccacca gatccgagtg cagcgccgca
     7801 acaccgttca ccgcgaaacc gccaaccaca cacaggttcg ccatatgcac ttgtttatcg
     7861 tgcaccaccg ccagtttggc ccacactttt tcatcgcccg gccaggtttt ctccaccagc
     7921 gttttaaagc gagtattaat ttcgttaata atctgcatat ggcgcggcag taagcctttc
     7981 accagtttca catcccagcg ttccagcgct tctggcatca gggtatggtt ggtgtaagcg
     8041 aaagttttgc tggtgatggc ccaggcgtca tcccagctca tctggtgctc atcggtcagc
     8101 acgcgcagca gttctggaat cgcgatagtt ggatgggtat cgttcagctg aataacttcg
     8161 taatccgcca gttcgtgcag tttacgcccc gccagatgat ggcgacgcaa aatatccgct
     8221 accgaacagg cacactggaa gtattgctgc atcaggcgca gctttttacc ggcagtatgg
     8281 ttgtcgtttg gatagagaac tttggtcagt ttttccgcat tgatgccttg ctgttcggcg
     8341 cgcaggaaat caccgtcgtt aaatttagtt agatcaaacg gatgcgcatg cgtcgcctgc
     8401 cacagacgca gcggctgcgc cacgccatta cgatagccga caacggggag atcccacgct
     8461 tgaccggtaa tggtaaactc cggctcccag cgtccgtctt tcatcacttt accgccaatc
     8521 cctacctgca catccagtgc ttcgttgtgg cggaaccacg ggtagttact gcgatgccag
     8581 tcatccggca cttcaacctg tttgccatcg acaaaagact ggcggaacaa accatattga
     8641 tagttcagac cgtaacccgt cgcagactga ccgacagttg ccattgagtc gaggaagcac
     8701 gccgccagac gtcccagacc accgttaccc agcgccgggt cgatctcttc ttccagcagg
     8761 tcagtcaggt tgatgtcata agccttcaac gaatcctgta catcctgata ccagccgaga
     8821 ttcaacaggt tgttgcccgt cagacgacca atcaaaaact ccattgagat gtagttaaca
     8881 tgtcgctgat tcgccaccgg tttggcgaat ggctgagcac gcagcatttc ggccagtgct
     8941 tcactcactg ccagccacca ctggcgagga gtcatttcag ccgcagaatt taagccataa
     9001 cgctgccact gacgtgaaag cacttcctga aattgcttat cgttaaaaat aggttgtgac
     9061 ataggagttc cacttttctt agattttcaa cacaacgtta tcgctagttt gccaggctcg
     9121 atgttgacct tcctcatcct gcgggggatt aggcagggag gagttgcggg gatgagcaag
     9181 gaaatgtgat ctcgaccact taaagctagt gcaaaccaca ggattagcag caaatcaatg
     9241 ccatacagcg cagaaaatct gtatctaagt gcaaaaaatg gccgttgcgt attttcaaaa
     9301 agcggaaggt aactctataa attaagtaaa ggagtgaaac agtttcacaa gtaaaatatc
     9361 cagtgtgctc catctcattc ttaatagatt tattaagatc atctttttag atggcacttt
     9421 catcaggaat gaagaagaaa cccttgctta aatgaatctg atgaacataa gggaaaccag
     9481 tattcacgct ggatcagcgt cgttttaggt gagttgttaa taaagatttg gaattgtgac
     9541 acagtgcaaa ttcagacaca taaaaaaacg tcatcgcttg cattagaaag gtttctggcc
     9601 gaccttataa ccattaatta cgaagcgcaa aaaaaataat atttcctcat tttccacagt
     9661 gaagtgatta actatgctga ttccgtcaaa actaagtcgt ccggttcgac tcgaccatac
     9721 cgtggttcgt gagcgcctgc tggctaaact ttccggcgcg aacaacttcc ggctggcgct
     9781 gatcacgagt cctgcgggct acggaaagac caccctcatt tcccagtggg cggcaggcaa
     9841 aaacgatatc ggctggtact cgctggatga aggtgataac cagcaagagc gtttcgccag
     9901 ctatctcatt gccgccgtgc agcaggcaac caacggtcac tgtgcgatat gtgagacgat
     9961 ggagcaaaaa cggcaatatg ccagcctgac gtcactcttc gcccagcttt tcattgagct
    10021 ggcggaatgg catagcccac tttatctggt catcgatgac tatcatctga tcactaatcc
    10081 agtgatccac gagtcaatgc gcttctttat tcgccatcaa ccagaaaatc tcaccctagt
    10141 ggtgttgtca cgcaaccttc cgcaactggg cattgccaat ctgcgtgttc gtgaccaact
    10201 gctggaaatt ggcagtcagc aactggcatt tacccatcag gaagcgaagc agttttttga
    10261 ttgccgtctg tcatcgccga ttgaagccgc agaaagcagt cggatttgcg atgacgtttc
    10321 cggttgggcg acggcactat agctaatcgc cctctccgcc cggcagaata cccactcagc
    10381 ccataagtcg gcacgccgcc tggcgggaat caatgccagc catctttcgg attatctggt
    10441 cgatgaggtt ttggataacg tcgatctcgc aacgcgccat tttctgttga aaagcgccat
    10501 tttgcgctca atgaacgatg ccctcatcac ccgtgtgacc ggcgaagaaa acgggcaaat
    10561 gcgtctcgaa gagattgagc gtcaggggct gtttttacag cggatggatg ataccggcga
    10621 gtggttctgc tatcacccgc tgtttggcaa cttcctgcgc cagcgctgcc agtgggaact
    10681 ggcggcggag ctgccggaaa tccaccgtgc cgccgcagaa agctggatgg cccagggatt
    10741 tcccagcgaa gcgattcatc atgcgctggc ggcaggcgat gcgctgatgc tgcgcgatat
    10801 tctgcttaat cacgcctgga gtttgttcaa ccatagcgaa ctgtcgctgc tggaagagtc
    10861 gcttaaggcc ctgccgtggg acagtttgct ggaaaatccg cagttagtgt tattgcaggc
    10921 gtggctaatg caaagccaac atcgctatgg cgaagttaac accctgctag cccgtgctga
    10981 acatgaaatc aaggacatca gagaaggcac catgcatgca gaatttaacg ctctgcgcgc
    11041 ccaggtggcg attaacgatg gtaatcagga tgaagcggaa cggctggcaa aactggcact
    11101 ggaagagctg ccgccgggct ggttctatag ccgcattgtg gcaacctcgg tgctgggtga
    11161 agtgctgcac tgcaaaggcg aattgacccg ctcactggcg ctagtgcagc aaaccgaaca
    11221 gatggcacgc cagcacgacg tctggcacta cgccttatgg agtttaattc agcaaagtga
    11281 aattctgttt gcccaagggt tcctgcaaac cgcgtgggaa acgcaggaaa aagcgttcca
    11341 gctgatcaac gagcagcatc tggaacagct gccaatgcat gagtttctgg tgcgcatccg
    11401 agcacaactg ttatgggcct gggcacggct ggatgaagcc gaagcgtcgg cgcgtagcgg
    11461 gattgaagtc ttgtcgtctt atcagccaca gcaacagctt cagtgcctgg caatgttgat
    11521 tcaatgctcg ctggcccgtg gtgatttaga taacgcccgt agccagttga accgtctgga
    11581 aaacctgctg gggaatggca aatatcacag cgactggatc tctaacgcca acaaagtccg
    11641 ggtgatttac tggcaaatga ccggcgataa agccgccgct gccaactggt tgcgtcatac
    11701 ggctaaaccg gagtttgcga acaaccactt cctgcaaggt caatggcgca acattgcccg
    11761 cgcacaaatc ttgctgggcg agtttgaacc tgcggaaatt gttctcgaag aactcaatga
    11821 aaatgcccgc agtctgcggt tgatgagcga tctcaaccgt aacctgttgc tgcttaatca
    11881 actgtactgg caggccggac gtaaaagtga cgcccagcgc gtgttgctgg acgcattaaa
    11941 actggcgaat cgcaccggat ttatcagtca ttttgtcatc gaaggcgaag cgatggcgca
    12001 acaactgcgc cagctgattc aacttaatac gctgccggaa ctggaacagc atcgcgcgca
    12061 gcgtattctg cgagaaatca atcaacatca tcggcataaa ttcgcccatt tcgatgagaa
    12121 tttcgttgaa cgtctgctaa atcatccgga agtgccagaa ctaatccgca ccagcccgtt
    12181 gacccaacgt gaatggcagg tcctggggct gatctactcc ggttacagca atgaacaaat
    12241 tgcgggcgaa ctggaagtgg cggcaaccac catcaagacg catatccgca atctgtacca
    12301 aaaactcggc gtcgcccacc gtcaggctgc cgtgcagcac gcgcagaaac tgctgaagat
    12361 gatgggttat ggggtgtgag tttagccgga taatgcattg gatccggttt acatctttac
    12421 atcattcaat actcacccgc gttacgccat ctgtttctat caaaccaaac cgcaccggca
    12481 agaaacgctc caccaccgcg atattggtca gcagatggca tgaaggatgg gcgaccgtaa
    12541 attctcccgc gcccgccagc gccatcggca gtaccagttg gtcggcgaga tattccccca
    12601 ccgccgctgg gcttgccagg tagcgtttca cctctttcac taactgcgcg gctaccacct
    12661 cggcactgac gcgcttttca ccgacgacga aaaagcgttc agtgatattt tcactttcga
    12721 cttccagcga gacggtatta cccggcccct ggtcgcgcgg caggttatga atattctgtt
    12781 catgcaggga aaaaatcccc gccagtgtag cgatttcacg ctcagcaaca tggcgcggca
    12841 caccagctaa tagaacttct ccacgcatct gcacaatgtt cccgcgctcg ccaagttgca
    12901 aggtgctaaa cgatgccacg ggcgagactt ccgttgccac cacaccgcct ccggcaggat
    12961 aaaaaccgtg acgtaacagc gtggtttgct gatgaattcc tattttcgcc agcagcggct
    13021 ccagcacccg gcggataaaa tccgcaggcg gcgccgacgg gttatcggtg ccgccgctca
    13081 cttcaacacg cgaaggtcca tcggcaaacc acagcgcggg cagcaccgtt tgcagcacca
    13141 gcgtacaact tccggcgcta ccgatagcaa agcggtaatc gccgccgcgc acggtgccag
    13201 gccggaagac cagacgctgc gatcccagct ccgcgccttc caccgttgcc ctacaaattt
    13261 ccgcagccgc ttttaccgcg gtcagatgct ggcgcagcag ccccggtttc gcccgtccgg
    13321 ctcgaatgcc agtgatggta aatggctggc cggttatcat cgacaggctc agcgccgagc
    13381 gcaggatctg cccgccgcct tcgccctgtg cgccatccag cgcaatcatc cttttcatca
    13441 tttatccttt tacgcacacc acctgacgca gggtgtagat aacttccacc agatcgcttt
    13501 gtgccgccat caccgcatca atatctttat acgccatcgg gatttcgtcg atcacttcgg
    13561 catctttacg gcattccaca tgcgcggtgg cacgaatttg gtcttccacg ctgaacagtt
    13621 ttttcgcttt agttcggctc attacccgcc cggcaccgtg gctgcacgaa cagaacgact
    13681 cttcatttcc cagcccacgg acgataaagc tttttgctcc catcgaaccg ggaataattc
    13741 catattgacc agcacgcgca gacaccgcac ctttacgcgt cacgtagatc tcttcaccaa
    13801 agagctgttc tttttgcaca tagttgtggt gacagttgat ctcttccatc gccagcgttt
    13861 gtggctgtct gaccgttttc tgcgtaatgc tctgcaacgc cgttaccaca ttttccatca
    13921 tcgcatcgcg gttaaggctg gcgaaaagct gcgcccaggc cacggctctt aggtaatcat
    13981 caaagtattc cgtaccttcc ataaagtacg ccagatcacg cgacggcaac gtctcaagtt
    14041 ggtcctgcat ctctttttgc gccagatcga taaagtaagt cccgatggcg ttaccaattc
    14101 cgcgtgaacc ggagtgcagc ataatccaca cctgctccga ctcatcaagg cagatttcaa
    14161 taaagtggtt acccgttccc agcgttccca ggtgtttata gttgttagtg ttaaggaaac
    14221 gcggatattt ttgcgttaac cactgataac cggcctccag ctcagcccat ttcgcatcga
    14281 cgttaaaagg cgggttttcc catgcgcctt tatcacgttt acaacgcccg gtagtacgcc
    14341 cgtgtggtac ggccgtttca atcgcttgac gcagctccgc caggttttca ggcaggtctg
    14401 ccgccgttaa cgcggtacgc agcgcgttca ttccacagcc aatatccacg cccaccgccg
    14461 ccggaataat cgcccctttg gtcgggatca cgctaccaat ggtggaacct ttacccaggt
    14521 gtacatcagg cattaccgca atatgtttga aaataaacgg catcttcgcc gtattaataa
    14581 gttgctgacg cgcatcggct tctaccggca cgcctttggt ccacattttt accggggcat
    14641 tttcagtggt cagtaattcg taattcattt tgtttttctc ttttcgttgt ttgcggtcct
    14701 gataattgca accgtcgtgc cagaaaatta aataagcaga ttaatttatt gtttctaaaa
    14761 agattatatc tttatatccg taaacggaga tttcccgcaa agccgattta ccgataatga
    14821 aatatcgcct tttataagga tatctaagat gcgtaaaaca gtggcttttg gctttgtcgg
    14881 taccgtgctg gattatgccg gacgcggcag tcagcgctgg tcaaaatggc gtccgtcact
    14941 ctgtatatac cagcaagaat cgttggtcat cgatcgactg gaattattgc acgatgcccg
    15001 ctcgcgctcg ctatttgaaa cgcttaaacg cgatatcgcc agcgtttcgc cagaaacaga
    15061 agtggtgggc gttgagattg aactgcataa cccgtgggat ttcgaagagg tttacgcctg
    15121 cctgcatgat ttcgcccgtg gttacgagtt tcagccagaa aaagaagact atttaattca
    15181 catcaccacc ggcacccacg tcgcgcagat ttgctggttt ctgctggcgg aagcgcgtta
    15241 tctacccgcc cggttgatac aatcttcaac tccgcgcaaa aaagaacagc cgcgcggccc
    15301 aggtgaagtg acgattatcg atctcgattt aagccgttat aacgccatcg ccagccgctt
    15361 tgccgaggaa cggcagcaaa cgctcgattt cctcaaatcc ggtattgcca cgcgtaaccc
    15421 ccacttcaac cgcatgattg agcagatcga aaaagtggcg attaaatccc gcgcgccgat
    15481 tctgcttaac ggcccgaccg gcgcaggtaa gtcatttctg gcgcgacgca tcttcgagtt
    15541 aaaacaggca cggcatcagt ttagcggcgc gtttgtggaa gtgaactgcg ccaccctgcg
    15601 cggcgatacc gcgatgtcga cgctgtttgg tcatgtgaaa ggcgcgttta ccggggcgcg
    15661 ggaatctcgt gaaggattat tacgcagcgc caacggcgga atgttgtttc ttgatgagat
    15721 tggcgaactg ggcgcagacg aacaggcaat gctgctgaaa gctattgaag agaaaacctt
    15781 ttacccgttt ggcagcgatc gccaggtgag cagtgatttt cagcttatcg ccggaacggt
    15841 gcgcgatttg cgccagctgg ttgccgaagg caaattccgc gaagatctgt acgcgcggat
    15901 caatctctgg accttcaccc tgccgggtct acgccagcgc caggaagata ttgaaccgaa
    15961 cctggattat gaagtggagc gccacgccac actcactggt gacagcgtgc gttttaacac
    16021 cgaagcgcgg cgtgcctggc tggcctttgc aacatctccc caggcaacat ggcgcggtaa
    16081 ctttcgcgaa ctttctgcca gcgtcacgcg gatggccacc tttgccacta gcggacgcat
    16141 cactctggac gtggtggaag atgagataaa ccgtctgcgc tataactggc aggagagccg
    16201 cccctccgcg cttacggcgt tgctgggcgc agaggcagaa aacatcgatc tcttcgaccg
    16261 tatgcaactg gaacacgtta tcgctatctg ccgccaggca aagtcgcttt ctgctgccgg
    16321 acgacagctt tttgatgttt cgcgccaggg caaagccagc gtcaatgacg cggatcggct
    16381 acgcaaatac ctggcgcgtt ttggtctgac gtgggaagcc gtgcaggatc agcacagctc
    16441 cagttgaata tggtggtccg tcagcacctg catcacgctt actggcggcg gggcgtcggt
    16501 gtagacggca tcaaccatgc tgatgctccc catattgacc atcgcattac ggccaaattt
    16561 tgaatggtca accaccagca taacgtggcg cgagttctca ataatggcgc gcttggtacg
    16621 aacttcgtgg taatcgaact ccagcagcga gccgtcgctg tcgatgccgc tgatccccag
    16681 aatgccgaaa tcaaggcgga actgggagat aaaatcgagc gtcgcttcgc caatgatccc
    16741 gccatcgcgg ctacgtaatt cgccaccggc gagaataatg cggaaatctt cttttaccat
    16801 caacgtgtta gcaacgttga gattgttggt gacaatgcgc aaattgctgt gattgagcag
    16861 cgcgtgcgcc accgcttccg gcgtggtgcc gatatcgata aacagcgtcg agccattggg
    16921 gatttgctcc gccactttgc gggcgatgcg ctctttttct tcggtctggg tggctttacg
    16981 atcgtgccac ggcgtgttaa ccgaactgga aggcagcgcc gcgccgccat gatggcgcag
    17041 gatcaggttt tgctctgcca gctcgttgag gtcgcggcga atagtctgcg ggctgacgga
    17101 gaagtgctcc accagttcct cggtactgac ataaccctgc tgtttaacca gttcgataat
    17161 accgttgtga cgttgtgttt gtttcattta taaatccctg gaattatttt cgttttcgcg
    17221 cattgagcga atcaacaaaa gccatcgcta aacccacggc taatccggcg atgtgtgctc
    17281 cgttcgccat cgacatccca aacaaatcaa accatccggc gacaatccag atcagcgcaa
    17341 agataattaa cccacgttgc aggtaaatgc cactttgcgg atcgcgttcg ccacgtagcc
    17401 agacgtagcc catcagcgca tacaccacgc cagaaagccc gccaaaccac ggcccgctga
    17461 atttttgctg catatagccg cttaacaggg cgctgataag cgtaatgaca attagcttac
    17521 cgctaccgag gcgtttttcc accgcgccgc cgagatacca ccaccaaagc aggttaaaga
    17581 ggatatgcat cagcgagaag tgcattaacg cgtgggtgaa gtaacgccag aactcaaatt
    17641 tcagtgttgg atcgaatggc caggccagcc ataacatcac ttcctgatcg ccgagaattt
    17701 gcatggcaat aaacaccacc acgcaggcga tcatcatcac ccaggtcacc ggacctgcgc
    17761 gttcacgcaa ggcagcaaag aaaggataac ggcgataatg caggccactg ccggtatggc
    17821 ctgccagcca gctggccgcc agataacgcg gatctgccgg gttttcgaga aaacgcgcca
    17881 gctccgcccg cacgcgctcg gcctgggact catccgccag ccagacatcg ctttggttat
    17941 gttgttgaat cgtgaggata acaccctgcg tcgccatgta atcaacaaac gcctgcgcca
    18001 cgcgggggtt agcaaaagag gtaatcatca acatcgttgc tgtcgcttat tccacacaaa
    18061 aggggacagt ataaagcgtt acgcgccgta cgccacctct gcgggaaact gtcgctgcca
    18121 ggcttcaaag ccgccgtcaa tgctatagac cacatcgtag ccctgttgca gcagatactg
    18181 cgccgcccct ttgctgctat tgccgtgata acacatcacc atcaccggag tgtcaaagtc
    18241 gttatcacgc ataaacgcgc ccagcgtgtc gttggttaaa tggaaagcct gcaccgcatg
    18301 ccccatagcg aaactctgtg gatcgcgaat atcgaccagc accgcctctt tttcctgcaa
    18361 cttctggtgc gcgtcggcaa cgttaataca ttcgaactga tccatgcgtc tctctttctt
    18421 tacaaacaag tgggcaaatt taccgcacag tttacgtcga agcggcagat aaacgccata
    18481 atgttataca tatcactcta aaatgttttt tcaatgttac ccaaagcgcg attctttgct
    18541 aatatgttcg ataacgaaca tttatgagct ttaacgaaag tgaatgaggg cagcatggaa
    18601 accaaagatc tgattgtgat aggtggcggc atcaatggtg ctggtatcgc ggcagacgcc
    18661 gctggacgcg gtttatccgt gctgatgctg gaggcgcagg atctcgcttg cgcgacctct
    18721 tccgccagtt caaaactcat tcacggtggc ctgcgctacc ttgagcacta tgaattccgc
    18781 ctggtcagcg aggcgctggc tgaacgtgaa gtgctgctga aaatggctcc gcatatcgcc
    18841 ttcccgatgc gttttcgcct gccacatcgt ccgcatctgc gcccggcgtg gatgattcgc
    18901 attggtctgt ttatgtacga tcatctgggt aaacgcacca gcctgccggg atcaactggt
    18961 ttgcgttttg gcgcaaattc agtgttaaaa ccggaaatta agcgcggatt cgaatattct
    19021 gactgttggg tagacgacgc ccgtctggta ctcgccaacg cccagatggt ggtgcgtaaa
    19081 ggcggcgaag tgcttactcg gactcgcgcc acctctgctc gccgcgaaaa cggcctgtgg
    19141 attgtggaag cggaagatat cgataccggc aaaaaatata gctggcaagc gcgcggcctg
    19201 gttaacgcca ccggcccgtg ggtgaaacag ttcttcgacg aggggatgca tctgccttcg
    19261 ccttatggca ttcgcctgat caaaggcagc catattgtgg tgccgcgcgt gcatacccaa
    19321 aagcaggcct acattctgca aaacgaagat aaacgcattg tgttcgtgat cccgtggatg
    19381 gacgagtttt ccatcatcgg caccaccgat gtggaataca aaggtgatcc gaaagcagtg
    19441 aagattgaag agagtgaaat caattacctg ctgaaagtgt ataacgcgca ctttaaaaag
    19501 cagctaagcc gtgacgatat cgtctggacc tactccggtg tgcgtccgct gtgtgatgat
    19561 gagtccgact cgccgcaagc cattacccgt gattacaccc ttgatatcca tgatgaaaac
    19621 ggcaaagcac cgctgctgtc ggtattcggc ggtaagctga ccacctaccg aaaactggca
    19681 gaacatgcgc tggaaaaact gacgccgtat tatcagggta ttggcccggc gtggacgaaa
    19741 gagagcgtgc tgccgggtgg tgccattgaa ggcgaccgcg acgattacgc cgctcgcctg
    19801 cgccgccgtt acccgttcct gactgaatcg ctggcacgtc actacacgcg cacttacggc
    19861 agcaacagcg agctgctgct cggcaatgcg ggaacggtaa gcgatctcgg ggaagatttc
    19921 ggtcatgagt tctacgaagc ggaactgaaa tacctggtgg atcacgaatg ggtccgccgc
    19981 accgacgatg ccctgtggcg tcgcacaaaa caaggcatgt ggctgaatgc ggatcaacaa
    20041 tctcgtgtga gtcagtggct ggtggaatat acgcagcaga agttgtcgct ggcgtcgtaa
    20101 attaacgtaa ggtgatcagg tcagatttca atctggcctg agactgatga caaacacaaa
    20161 actgcctgat gcgctacgct tatcaggcct acgtggttta tgcaatatat tgaatttgca
    20221 tggtcttgta ggccagataa gacgttcacg tcgcatccgg catgaacaaa gcgcactttg
    20281 tcaaaaatct aacctacttt ttaattcagg aaattaccgc aaagcccacg ccatcatacc
    20341 caacgtaaca aaactcaggc acgttcccca ctcgccccga gaaaaatagc attaatgcgc
    20401 ccagcgtcag cataaaaata ttgagcagaa ggtaaattgt tggcgtaaag agtaaattgc
    20461 aaacctcagg aatagcggca agcccagccc ccagcatggc aataaccgct gcaaaaaatt
    20521 gcgtcagtat tgggatgccg aaaaaagtca ttaccagcga ggtcaaaatc cattttctat
    20581 tttgcattat tctttccatt ctttctgaat ggcgaaatta tactccccga gtccccttgc
    20641 cccttctgga cacttttccg aaatgatggc gaaaaaaacg ggaccctttg g
//
LOCUS       DAEBXN010000074        20612 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.074, whole genome shotgun sequence.
ACCESSION   DAEBXN010000074 DAEBXN010000000
VERSION     DAEBXN010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20612
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.074"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            96..395
                     /locus_tag="KJL99_002719"
     CDS             96..395
                     /locus_tag="KJL99_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBD4437394.1"
                     /translation="MTELAQLQASTEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            405..929
                     /gene="ygaP"
                     /locus_tag="KJL99_002720"
     CDS             405..929
                     /gene="ygaP"
                     /locus_tag="KJL99_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HBD4437395.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILNGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(976..1380)
                     /gene="stpA"
                     /locus_tag="KJL99_002721"
     CDS             complement(976..1380)
                     /gene="stpA"
                     /locus_tag="KJL99_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HBD4437396.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            2048..2497
                     /gene="alaE"
                     /locus_tag="KJL99_002722"
     CDS             2048..2497
                     /gene="alaE"
                     /locus_tag="KJL99_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HBD4437397.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(2534..2878)
                     /locus_tag="KJL99_002723"
     CDS             complement(2534..2878)
                     /locus_tag="KJL99_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HBD4437398.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            3030..3260
                     /locus_tag="KJL99_002724"
     CDS             3030..3260
                     /locus_tag="KJL99_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4437399.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVG"
     gene            3607..3852
                     /gene="nrdH"
                     /locus_tag="KJL99_002725"
     CDS             3607..3852
                     /gene="nrdH"
                     /locus_tag="KJL99_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HBD4437400.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            3849..4259
                     /gene="nrdI"
                     /locus_tag="KJL99_002726"
     CDS             3849..4259
                     /gene="nrdI"
                     /locus_tag="KJL99_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HBD4437401.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            4232..6376
                     /gene="nrdE"
                     /locus_tag="KJL99_002727"
     CDS             4232..6376
                     /gene="nrdE"
                     /locus_tag="KJL99_002727"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HBD4437402.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSCITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            6386..7345
                     /gene="nrdF"
                     /locus_tag="KJL99_002728"
     CDS             6386..7345
                     /gene="nrdF"
                     /locus_tag="KJL99_002728"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HBD4437403.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNTNKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            7700..8902
                     /gene="proV"
                     /locus_tag="KJL99_002729"
     CDS             7700..8902
                     /gene="proV"
                     /locus_tag="KJL99_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HBD4437404.1"
                     /translation="MAIKLKIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            8895..9959
                     /gene="proW"
                     /locus_tag="KJL99_002730"
     CDS             8895..9959
                     /gene="proW"
                     /locus_tag="KJL99_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HBD4437405.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPADGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALITWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            10017..11009
                     /gene="proX"
                     /locus_tag="KJL99_002731"
     CDS             10017..11009
                     /gene="proX"
                     /locus_tag="KJL99_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HBD4437406.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            11201..12380
                     /locus_tag="KJL99_002732"
                     /pseudo
     CDS             11201..12380
                     /locus_tag="KJL99_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            12504..13241
                     /gene="ygaZ"
                     /locus_tag="KJL99_002733"
     CDS             12504..13241
                     /gene="ygaZ"
                     /locus_tag="KJL99_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HBD4437407.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALSVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            13231..13566
                     /gene="ygaH"
                     /locus_tag="KJL99_002734"
     CDS             13231..13566
                     /gene="ygaH"
                     /locus_tag="KJL99_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HBD4437408.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            13657..14187
                     /gene="emrR"
                     /locus_tag="KJL99_002735"
     CDS             13657..14187
                     /gene="emrR"
                     /locus_tag="KJL99_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HBD4437409.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRKSDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            14314..15486
                     /gene="emrA"
                     /locus_tag="KJL99_002736"
                     /pseudo
     CDS             14314..15486
                     /gene="emrA"
                     /locus_tag="KJL99_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
     gene            15503..17041
                     /gene="emrB"
                     /locus_tag="KJL99_002737"
     CDS             15503..17041
                     /gene="emrB"
                     /locus_tag="KJL99_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HBD4437410.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLNSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDCFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFCASAWPQFIQGFAVVCFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(17105..17620)
                     /gene="luxS"
                     /locus_tag="KJL99_002738"
     CDS             complement(17105..17620)
                     /gene="luxS"
                     /locus_tag="KJL99_002738"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HBD4437411.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILEREVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(17770..19326)
                     /gene="gshA"
                     /locus_tag="KJL99_002739"
     CDS             complement(17770..19326)
                     /gene="gshA"
                     /locus_tag="KJL99_002739"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HBD4437412.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(19399..19827)
                     /locus_tag="KJL99_002740"
     CDS             complement(19399..19827)
                     /locus_tag="KJL99_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBD4437413.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(19824..20390)
                     /gene="yqaB"
                     /locus_tag="KJL99_002741"
     CDS             complement(19824..20390)
                     /gene="yqaB"
                     /locus_tag="KJL99_002741"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HBD4437414.1"
                     /translation="MYERYAGLIFDMDGTILDTAPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT         4973 a         5273 c         5149 g         5217 t
ORIGIN      
        1 agcttacgct tttactctca tttacgctgc tgaggctggt atatgttgca tttttagttt
       61 agagtttact taatttagaa aatacttaaa taaatatgac tgaactcgcg caattacagg
      121 ccagtaccga acaggcagcg gccttattga aagcaatgag ccaccctaaa cggttgctga
      181 ttctgtgcat gcttagcggt tcccccggca ccagcgcggg agagctgacg cgcattaccg
      241 gactgagcgc ctctgcgaca tcacagcatc tcgctcgtat gcgggacgaa gggcttatca
      301 acagccaacg ggatgcccaa cgcattctat attccattaa aaatgaggcg gtaaatgcca
      361 ttatcgccac cctgaaaaat gtctattgtc cgtaaggagt cactatggct ttgacaacca
      421 tttcgccgca tgatgcacaa gaattaatcg cacgcggcgc aaagttaatc gatattcgtg
      481 acgctgatga atatcttcgt gaacatattc ctgaagcaga tctggctcca ttatccgtgc
      541 tggaacagtc aggtcttcca cctaaattac gtcgcgagca aattatattc cactgccagg
      601 caggtaaacg caccagtaat aacgccgata aattagcagc gattgccgcc cccgcagaaa
      661 tatttttact cgaagatggg attgatggct ggaaaagagc tggattgcca gtagcggtaa
      721 ataaatctca acccttgccg ttaatgcgcc aggtgcagat cgctgcgggt ggtttaatat
      781 taaacggcgt tgtactgggt tataccgtaa atagcggttt cttcttatta agtggctttg
      841 ttggtgccgg gttactgttt gcaggaatca gcggtttttg tggaatggca aggttgttag
      901 ataagatgcc gtggaaccaa cgagcttgag aagcgacgcc ggacgcgccc tagcagcgac
      961 atccggcctc agtaattaga tcaggaaatc gtcgagagat ttaccttctg ccagcgcctg
     1021 agcaattggc ttcggtgtac ggccctgacc ggtccaggtt ttagtttcac cgttaacatc
     1081 agtgaattta tatttcgccg gacgcggctg gcgtttttta ccagcacgtg gtgcagcagc
     1141 agagctatta cccaataact cttccgggtt aattccgtca gctttcatca gctccagcca
     1201 ggtgctaatt ttttcctgac gctcagccag ttcacgctgc tgctgttctt cttcttcacg
     1261 tctttcttta gtgacaaccc tgaatttttc gagcatttct tcaagaacgt caatggagaa
     1321 ttcgcgagcc atcgcacgga gggtgcgaat gttatttaaa ctttgtaaca ttacggacat
     1381 aataaagaaa accttttaac gccaaaacaa aaaagtattt cgtcgcgtag tgtaagcgat
     1441 cacttattat atttccacaa aatttaatct atttacagtc ctgcgcgaga ttatttcagc
     1501 tgttattttc tgaaactctc agaataattt caagattata tattagccat tttgatgagt
     1561 aatttaggtt attggtgaac agagtggcgc tctctgctcg ctaactccgc aagcctgaca
     1621 aaaccgaagt gaataaaaat acaatataac agaataaaag aaaacacccc tcactggtgc
     1681 tttctgctgt taatgtacat tttctggcat caaaaatgct gtaaatgacc cggtcatgac
     1741 aaacaccgaa ttctctacgc ttatagcgtt acctcccccc caaactttca ttagtgaaat
     1801 taaaggcaag atcatatcta attgatttaa ttgaaaataa atcatattgg catgtaaaaa
     1861 tccacaatgt acaaaaaacg agccgttacg gaatatttta tctacaaaaa ctaactaaat
     1921 aaaaattttt cactaattga ttagtcatag gcagcgatat acgctatgca aaaatgcaga
     1981 tggcaatgag atccactgct ttcatctcca ttaacatccc attacgcttt tattaaggag
     2041 cattagcatg ttctcaccgc agtcacgctt gcgtcatgca gttgcagata cgttcgcgat
     2101 ggttgtttac tgttctgtcg tgaacatgtg tattgaagtt ttcctctccg gaatgagctt
     2161 cgaacagtct ttttattcca gattggtagc gattccggtg aacatcttaa ttgcatggcc
     2221 atacggtatg taccgcgatc tgtttatgcg cgcggcacgc aaagttagcc cgtcgggctg
     2281 gataaaaaat ctggctgata tcctggctta tgtgacgttc cagtcaccgg tgtatgtggc
     2341 gatcttgtta gtggtgggcg cagactggca tcagattatg gcggcggtca gttcaaacat
     2401 cgttgtttcg atgttgatgg gggcggttta tggctacttc ctcgattatt gccgccgact
     2461 gtttaaagtc agccgttacc agcaggtaaa agcctgactc ttcttttcgc gactggcatc
     2521 gccagtcgcg tctttaactg gcttcgccaa ataaaccatt caaataacgt tcaagcgcaa
     2581 cacgcgaact aaagccatgc gggataccgt aatgctcgtg tcgctcgcct gctaaataga
     2641 gcggaaattg ctggtaatga tcgcaagttt taatatcgga agcgggttca gccagtgttg
     2701 aactgcgttc ttttaatgtt ggatgaatta ccagagcggt acgtcccatt cgcgcttcgc
     2761 gattaacata gacataattt tccccacggc ggtaaccata cgctttctgg gttaccacat
     2821 cgacagtaaa accgactttt tcaagtacgc gcgccacctc gtctggtcgt aaatacatat
     2881 ttgatcctcg ttattttcaa ccgcgggcct accttacctg attgcgcatt cacaacgctt
     2941 tcagaaaagt ccataaagcc gcgatgaacc tcagttaaga aaattatggt ctacactgaa
     3001 aattacatcg aattctaatg ggggatcata tgtttaaccg accgaaccgc aacgacgttg
     3061 atgatggcgt gcaggatatc cagaatgatg tcaatcaatt agctgacagc ctcgaatctg
     3121 tattgaagtc ctggggcagc gacgccaaag gggaagctga agccgcacgc agtaaagctc
     3181 aggcgttgct gaaagaaacc agggcgcgaa tgcatggtcg tactcgcgtc cagcaagccg
     3241 cgcgcgatgc cgttggctga gcggattctt ttgttcgtga aagaccctgg tgtagcgtgg
     3301 gtacagcagc tgcggtaggt atttttatcg gcgcactgtt aagcatgcgc aaatcgtagt
     3361 gcaaaaatga taataaatac gcgtctttga ccccgaagcc tgtcttcggg gtttcttttt
     3421 gcctggtgaa tcacaaaaat ccccctcccc cgtcacgctc atatccaggg taatttcgat
     3481 cactatttgc tatatattgt gtagttgaat cttttttcaa ctacatctag tatctctgta
     3541 tcaacagaga gacaacccga cgcgtatcat cgcgccgtat cttcatttta aacggaaata
     3601 cgaatcatgc gcataactat ttatactcgt aacgattgcg ttcagtgcca cgccaccaaa
     3661 cgggcgatgg aaaaccgggg ctttgatttt gaaatgatta atgtcgatcg cgttcctgaa
     3721 gcagcggaag cgttgcgtgc tcagggcttt cgtcagttgc cggtagtgat tgctggcgat
     3781 cttagctggt ctggtttccg tccggacatg attaaccgtc tgcatccagc gccacacgcg
     3841 gccagtgcat gagccagctc gtctacttct ccagcagctc cgaaaacacg cagcgtttta
     3901 tcgaacgttt aggtctgccc gcggtgcgca tcccgctcaa tgagcgggaa cggattcagg
     3961 tagacgagcc ttacatcctg atcgtgccct cttacggcgg cggcggtacg gctggcgcgg
     4021 tgccacgaca ggtaattcgc tttttaaacg acgagcacaa ccgggcgttg cttcgcggcg
     4081 ttattgcttc tggtaatcgc aactttggtg aggcgtatgg ccgcgccgga gatgtgattg
     4141 cccggaaatg cggcgtgccg tggctgtacc gttttgaact catgggtacg caaagcgata
     4201 tcgaaaacgt tcgtaaagga gtaaccgaat tttggcaacg acaaccgcag aatgcctgac
     4261 gcaggaaacg atggattacc acgcgctgaa tgcgatgctt aatctttacg atagcgcagg
     4321 tcgcattcag ttcgataaag accgccaggc cgttgacgcc tttattgcga cgcatgtgcg
     4381 tccgaacagt gtgaccttca gtagccagca gcagcgcctg aactggctgg tcaacgaagg
     4441 ttactatgat gaaagcgttc ttaatcgcta ctctcgcgac tttgtcatta cgctgtttgc
     4501 ccacgcacac accagcggtt ttcgtttcca gacattcctc ggggcatgga agttttacac
     4561 cagctatacg ttgaagacat tcgacggtaa acgttatctg gaagattttg ccgatcgagt
     4621 cacgatggtg gcgctgacgc tggcacaagg cgatgagacg ctggcgttgc aactgaccga
     4681 tgaaatgctg tcaggacgct ttcagccagc cacgccaaca ttcctcaact gcggtaagca
     4741 gcagcgcggc gaactggttt cctgtttttt gctgcgtatt gaagacaata tggagtcgat
     4801 tggtcgggcg gtaaattccg cactgcagct gtcgaaacgc ggcggcggcg tagcattttt
     4861 gctgtcgaat ctgcgggaag cgggcgcgcc aattaaacgt attgaaaatc aatcttctgg
     4921 cgtaattccg gtgatgaaaa tgttggaaga cgcattttcc tatgccaacc aactcggcgc
     4981 gcgtcagggg gcaggtgcag tctatttaca tgctcatcat cccgatattc tgcgttttct
     5041 cgacacgaaa cgggaaaatg ccgacgaaaa aatccgcatt aaaacactgt cgctgggcgt
     5101 ggtgatcccg gatatcactt tccatctggc aaaagagaat gcgcagatgg cgctgttttc
     5161 gccctatgac gttgagcgag tttatggcaa gccgtttgcc gatatcgcca ttagcgaaca
     5221 ctatgacgaa ctggttgccg atgaacgcat ccgcaaaaaa tacctcaacg cccgtgattt
     5281 cttccagcga ctggcagaaa tccagtttga gtccggctat ccctacatca tgtatgaaga
     5341 cacagtaaac cgtgctaacc ctatcgccgg gcgcataaat atgagtaatc tctgctcaga
     5401 aattttgcag gttaacagcg cctcagagta tgacgagaat ctcgactatg cccgcacagg
     5461 ccatgatatt tcctgcaatt taggttcgtt gaatattgcg cacaccatgg attcccccga
     5521 tttttcccgc acggtagaga ctgccgtgcg cggtttaacg gcggtgtcag atatgagtca
     5581 tatccgcagc gtgccgtcca tcgaagccgg aaatgccgcc tcgcacgcca tcggactggg
     5641 gcagatgaat ttacatggtt atctggcgag ggaaggcatc gcttatggtt cgccggaagc
     5701 cctggatttc accaatctct atttctatac catcacctgg cacgcactgc gtacctcgat
     5761 gttgctggcg cgcgaacgcg gtgaaacctt cgccgggttc aaacagtcac gctatgccag
     5821 tggtgaatat ttcagccaat atctgcaagg taactggcag ccgaaaacgg cgaaagttgg
     5881 cgaactgttt gcccgcagct gtattacgtt acctacccgt gagatgtggg cgcaactgcg
     5941 cgacgacgtg atgcgctacg gcatatacaa ccagaatctc caggcggtgc cgccaaccgg
     6001 gtctatctct tatatcaacc atgctacgtc gagtattcat ccgattgtgg cgaaagtaga
     6061 gatacgcaaa gagggcaaaa caggacgcgt ttactaccct gccccgttta tgactaacga
     6121 aaatctggcg ctgtatcagg acgcttacga aattggcgca gaaaagatca tcgacaccta
     6181 cgcggaagcg actcgccatg tcgatcaggg gctgtcgctg acgctgtttt tccccgatac
     6241 cgccaccact cgcgatatca acaaagcgca gatttacgcc tggcgcaaag gtatcaaaac
     6301 gctctattac atccgcctgc gtcagatggc gctggaaggc actgaaattg aaggctgcgt
     6361 ctcctgtgca ctttaaggaa tatctatgaa actctcacgt atcagcgcca tcaactggaa
     6421 caagatatct gacgataaag atctggaggt gtggaatcgc ctgaccagca atttctggct
     6481 accagaaaag gtgccgctgt cgaacgatat tcccgcctgg cagacattaa ctgtcgtaga
     6541 acaacaactg acgatgcgcg tttttactgg cctgacgctg ctcgacacgc tgcaaaatgt
     6601 tatcggcgcg ccttcgctga tgcccgatgc gctcacgcct catgaagagg cggtattatc
     6661 gaatatcagc tttatggaag cggttcatgc ccgctcttac agttcgattt tctcgacgct
     6721 gtgccagacc aaagatgtcg atgccgccta cgcctggagt gaagaaaacg caccgttgca
     6781 gcgaaaagct cagattattc agcaacatta tcgcggtgat gatccgctga aaaagaaaat
     6841 cgccagtgtg tttcttgaat cttttttgtt ctattccggt ttctggctgc cgatgtattt
     6901 ttccagccgc ggaaagctga ccaataccgc ggacctgatc cgtctgatta tccgcgatga
     6961 agcagtccac ggttactaca taggctataa atatcagaaa aacatggaaa agatatctct
     7021 gggacaacgt gaagagttga agagtttcgc cttcgatttg ttgctggaac tctacgacaa
     7081 cgagttgcaa tacaccgatg agctgtacgc cgaaaccccg tgggctgacg atgtgaaagc
     7141 gtttctctgt tacaacacca ataaggcttt gatgaatctg ggctacgaac cgttatttcc
     7201 cgcagaaatg gcggaagtga atccggcaat cctcgccgcg ctttcgccga atgccgatga
     7261 aaatcacgat ttcttttccg gttcaggctc ctcttatgtg atggggaaag cggttgaaac
     7321 agaagatgaa gactggaatt tctgagggtg ttattttcaa aaatatcact acccgcagca
     7381 gggaaataat tcccgccaaa tagcttttta tcacgcaaat attctgtagt gatctacact
     7441 gatactctgt tgcattattc gcctgaaact acaatattca ggcgtttttt cgctatctat
     7501 gacaaaaaat atcaactttt ctcgatttgc tctcagccct tatatcacgg aaaattccgg
     7561 cgatttgctc gcatcaatat tcatgccaca tttgccctca ggggttgcct cagattctca
     7621 gtatgttagg gtagaaaaaa gtgactattc ccattgggta atatatcgac ataaacaaat
     7681 aaagaaatca ttctattgca tggccattaa attaaaaatt aaaaatcttt ataaaatatt
     7741 tggtgagcat ccacagcgag cgttcaaata tatcgaacaa ggactttcaa aagaacaaat
     7801 tctggaaaaa actgggctat cgcttggcgt aaaagacgcc agtctggcca ttgaagaagg
     7861 cgagatattt gtcatcatgg gattatccgg ctcgggtaaa tccacaatgg tacgccttct
     7921 caatcgcctg attgaaccca cccgcgggca agtgctgatt gatggtgtgg atattgccaa
     7981 aatatccgac gccgaactcc gtgaggtgcg cagaaaaaag attgcgatgg tcttccagtc
     8041 ctttgcctta atgccgcata tgaccgtgct ggacaatact gcgttcggta tggaattggc
     8101 cggaattaat gccgaagaac gccgggaaaa agcccttgat gcactgcgtc aggtcgggct
     8161 ggaaaattat gcccacagct acccggatga actctctggc gggatgcgtc aacgtgtggg
     8221 attagcccgc gcgttagcga ttaatccgga tatattatta atggacgaag ccttctcggc
     8281 gctcgatcca ttaattcgca ccgagatgca ggatgagctg gtaaaattac aggcgaaaca
     8341 tcagcgcacc attgtcttta tttcccacga tcttgatgaa gccatgcgta ttggcgaccg
     8401 aattgccatt atgcaaaatg gtgaagtggt acaggtcggc acaccggatg aaattctcaa
     8461 taatccggcg aatgattatg tccgtacctt cttccgtggc gttgatatta gtcaggtgtt
     8521 cagtgcgaaa gatattgccc gccggacacc gaatggctta attcgtaaaa ctcctggctt
     8581 cggcccacgt tcggcactga aattattgca ggatgaagat cgcgaatatg gctacgttat
     8641 cgaacgcggt aataagtttg tcggcgcagt ctccatcgat tcgcttaaaa ccgcgttaac
     8701 gcagcagcaa ggtcttgatg cggcgctgat tgatgcgccg ttagcagtcg atgcacaaac
     8761 gcctcttagc gagttgctct ctcatgtcgg acaggcaccc tgtgcggtgc ccgtggtcga
     8821 cgaggaccaa cagtatgtcg gcatcatttc gaaaggaatg ctgctgcgcg ctttagatcg
     8881 tgagggggta aataatggct gatcaaaata atccgtggga taccacgcca gcggcggaca
     8941 gtgccgcgca atccgcagac gcctggggta caccgacgac tgcaccggct gacggcggtg
     9001 gtgctgactg gctgaccagt acgcctgcgc caaacgtcga gcattttaat attctcgatc
     9061 cgttccataa aacgctgatc ccgctcgaca gttgggtcac tgaagggatc gactgggttg
     9121 ttacccattt ccgtcccgtc ttccagggcg tgcgcgttcc ggttgattat atcctcaacg
     9181 gtttccagca attgctgctg ggtatgccag cgccagtggc gattatcgtt ttcgctctca
     9241 tcacctggca gatttccggg gtcggaatgg gcgtggcgac gctggtctcg ctgattgcca
     9301 tcggcgcaat cggtgcctgg tcgcaggcaa tggtgactct ggcgctggtg ttaactgccc
     9361 tgctgttctg tatcgtcatc ggtttgccgt tggggatatg gctggcgaga agtccgcgag
     9421 cggcgaaaat tattcgtcca ctgcttgatg ccatgcagac cacgccagcg tttgtttatc
     9481 tggtgccaat cgtcatgcta tttggtatcg gtaacgtgcc gggcgtggtg gtgacgatca
     9541 tctttgcgct gccgccgatt atccgtctga ccattctggg gattaaccag gttccggcgg
     9601 atctgattga agcctcgcgc tcattcggtg ccagcccgcg ccagatgctg ttcaaagttc
     9661 agttaccgct ggcgatgccg accattatgg cgggcgttaa ccagacgctg atgctggccc
     9721 tttctatggt ggtcattgcc tcgatgattg ccgtcggcgg gctgggtcag atggtacttc
     9781 gcggtatcgg tcgtctggat atggggcttg ccaccgttgg cggcgtcggg attgtgatcc
     9841 tcgccattat cctcgatcgc ctgacgcagg ccgttgggcg cgactcacgc agtcgcggca
     9901 accgtcgctg gtacaccact ggccctgttg gtctgctgac ccgcccattc attaagtaac
     9961 tctgcacttg cccggtgacg ccgggcatta tcaccctgcc aaaaaaagga ataacaatgc
    10021 gacatagcgt actttttgcg acagcgtttg ccacgcttat ctctacacaa acttttgctg
    10081 ccgatctgcc gggcaaaggc attactgtta atccagttca gagcaccatc actgaagaaa
    10141 ccttccagac gctgctggtc agtcgtgcgc tggagaaatt aggttatacc gtcaacaaac
    10201 ccagcgaagt ggattacaac gttggctaca cctcgcttgc ttccggcgat gcaaccttca
    10261 ccgccgtgaa ctggacgcca ctccatgaca acatgtacga agctgccggt ggcgataaga
    10321 aattttatcg tgaaggggta tttgttaacg gcgcagcaca gggttacctg atcgataaga
    10381 aaaccgccga ccaatacaaa atcaccaaca tcgcacaact gaaagatccg aagatcgcca
    10441 aactgttcga taccaacggc gacggtaaag cggatttaac cggttgtaac cctggctggg
    10501 gctgcgaagg tgcgatcaac caccagcttg ccgcgtatga actgaccaac accgtgacgc
    10561 ataatcaggg gaactacgca gcgatgatgg ccgacaccat cagtcgctac aaagagggca
    10621 aaccggtgtt ttattacacc tggacgccgt actgggtgag taatgagctg aagccaggga
    10681 aagatgtggt ctggttgcag gtgccgttct ccgcactgcc gggcgataaa aatgccgata
    10741 ccaaactgcc gaatggcgcg aattacggct tcccggtcag caccatgcat atcgttgcca
    10801 acaaagcctg gaccgagaaa aatccggcgg cagccaaact gtttgccatt atgcagttgc
    10861 cagtggccga tattaacgcc cagaacgcca ttatgcatga cggcaaagcc tcagaaggcg
    10921 atattcaggg ccacgttgat ggctggatca aagcccacca gcagcagttc gatggctggg
    10981 tgaatgaggc gctggcagcg cagaagtaat ttttattcgg gcggataagg cgttttcgcc
    11041 tcatccgccg ctctgtacac aatgcctgat gcgacgctga cgcgtcctat catgcctaca
    11101 aaccacatcg cacatttcaa caagctatct ttcatcccat attcatcaac atccgctatt
    11161 attgatttcc agcttaatca tcacctgatg aacaaaaata atgactaaac ctaatcatga
    11221 gcttagcccg gcgctgatcg tgctgatgtc tatcgccacc ggtctggcgg tagccagtaa
    11281 ctattacgcc cagccattgc tcgacaccat cgcgcgtaac ttttcccttt ccgccagttc
    11341 ggcaggcttt attgttaccg ccgcgcagtt gggctatgcc gcaggtctac tgtttcttgt
    11401 tcccctcggt gatatgtttg aacgccgccg cctgattgtc tcgatgacct tactggcagc
    11461 gggcggtatg ttaatcactg ccagcagtca gtcactggcg atgatgatcc tcggtacggc
    11521 attaaccggt ttattctcag tcgtggcaca aattctggtt ccgctggcag cgacgctggc
    11581 ttcaccggat aaacgcggca aagtggttgg caccattatg agcggtctgc tgttggggat
    11641 attgctggca cgaaccgttg ccgggttact ggcaaacctc ggcggctggc gcaccgtctt
    11701 ttgggttgct tcggtgttaa tggcactgat ggcgctggca ttatggcgtg gtctgccaca
    11761 aatgaaatca gaaacccacc tcaactaccc acagttgttg ggttccgttt tcagcatgtt
    11821 catcagcgat aagatcctgc gcacccgcgc gttgctgggc tgcatgacct ttgccaactt
    11881 cagcattctc tggacctcaa tggccttttt gcttgccgct ccacctttta actacagcga
    11941 tggtgacatt ggtctgtttg ggcttgcggg agctgccgga gcgttgggcg ctcgtccggc
    12001 gggcggtttt gccgacaagg gcaaatcgca ccacaacttt cggtctgctt ctgctgttac
    12061 tttcatggct ggcaatctgg tttggtcaca cttccgtact ggcgttgatt atcggcattc
    12121 tggtgctgga cctcaccgta cagggcgtgc atatcactaa ccagacggta atttatcgaa
    12181 tacatcctga tgcgcgtaat cgcctgaccg caggttacat gaccagctac tttattggcg
    12241 gtgccgccgg ttcgctaatt tcagcctcag cctggcaaca tggcggttgg gctggcgttt
    12301 gtctggctgg cgcgacgatt gccctggtta acttactggt ctggtggcga ggttttcatc
    12361 gtcaggaagc cgcaaattaa gcaatcagca accgttttcg tggctttaca cttataaggg
    12421 tgttaagaag cccatcagtc tgataaggtt aagatattca ttcagtctat ttataatatt
    12481 aacaatcgtt aagcgtacac tctatggaaa gccctactcc acagcctgct cctggttcgg
    12541 cgaccttcat ggaaggatgc aaagacagtt taccgattgt tattagttat attccggtgg
    12601 cctttgcgtt cggtctgaat gcgacccgtc tgggattctc tcctctcgaa agcgtttttt
    12661 tctcctgcat catttatgca ggcgcgagcc agttcgtcat taccgcgatg ctggcagccg
    12721 ggagtagttt gtgggttgct gcactgagcg tcatggcaat ggatgttcgc catgttttgt
    12781 atggcccgtc actgcgtagc cgtattattc agcgtctgca aaaatcgaaa accgccctat
    12841 gggcgtttgg cctgacggat gaggtttttg ccgccgccac cgcaaagctg gtacgcaata
    12901 atcgccgctg gagcgagaac tggatgatcg gcattgcctt cagttcatgg tcatcgtggg
    12961 tatttggtac ggtaataggg gcattctccg gcagcggctt gctgcaaggt tatcccgccg
    13021 ttgaagctgc attaggtttt atgcttccgg cactctttat gagtttcctg ctcgcctctt
    13081 tccagcgcaa acaatctctt tgcgttaccg cagctttagt tggtgccctt gcaggcgtaa
    13141 cgctattttc tattcccgtc gccattctgg caggcattgt ctgtggctgc ctcactgcgt
    13201 taatccaggc attctggcaa ggagcgcccg atgagctatg aggttctgct gcttgggtta
    13261 ctggttggcg cggcgaatta ttgcttccgc tatttgccgc tgcgcctgcg tgtgggtaat
    13321 gcccgcccaa ccaaacgtgg cgcggtaggt attttgctcg acaccattgg catcgcctcg
    13381 atatgcgctc tgctggttgt ctctaccgca ccagaagtga tgcacgatac acgccgtttc
    13441 gtgcccacgc tggtcggctt cgcggtactg ggtgccagtt tctataaaac acgcagcatt
    13501 atcatcccaa cactgcttag tgcgctggcc tatgggctcg cctggaaagt gatggcgatt
    13561 atataaccca caagaatcat ttttctaaaa caatacattt actttatttg tcactgtcgt
    13621 tactatatcg gctgaaatta atgaggtcat acccaaatgg atagttcgtt tacgcccatt
    13681 gaacaaatgc taaaatttcg cgccagccgc cacgaagatt ttccttatca ggagatcctt
    13741 ctgactcgtc tttgcatgca catgcaaagc aagctgctgg agaaccgcaa taaaatgctg
    13801 aaggctcagg ggattaacga gacgttgttt atggcgttga ttacgctgga gtctcaggaa
    13861 aaccacagta ttcagccttc tgaattaagt tgtgctcttg gatcatcccg taccaacgcg
    13921 acgcgtattg ccgatgaact ggaaaaacgc ggttggatcg aacgtcgtaa aagcgataac
    13981 gatcgccgct gcctgcatct gcaattaacg gaaaaaggtc acgagttttt gcgcgaggtt
    14041 ttaccaccgc agcataactg cctgcatcaa ctctggtccg cgctcagcac aacagaaaaa
    14101 gatcagctcg agcaaatcac ccgcaaattg ctctcccgtc tcgaccagat ggaacaagac
    14161 ggtgtggttc tcgaagcgat gagctaacgc gtcatctcgc tcaaaaatcc agatttataa
    14221 aagaaaaaat gaccggccag catcgcaaca tgctggcctt tttggcaagc aggtcggctc
    14281 agccgatgag ttaagaagat cgtggagaac aatatgagcg caaatgcgga gactcaaacc
    14341 ccgcagcaac cggtaaagaa gagcggcaaa cgtaagcgtc tgctcctcct tctcaccttg
    14401 ctctttataa ttattgccgt agcgataggg atttattggt ttttggtact gcgtcatttc
    14461 gaagaaaccg atgacgcata cgtggcaggg aatcagattc aaattatgtc tcaggtgtct
    14521 ggcagcgtga cgaaagtctg ggccgataac accgattttg taaaagaagg cgatgttctg
    14581 gtcactctcg acccgacaga tgctcgccag gcgtttgaaa aagccaaaac tgcactggct
    14641 tccagcgttc gccaaaccca ccagctgatg attaacagca aacagttgca ggcgaatatt
    14701 gaggtgcaaa aaatcgccct cgcgaaagca caaagcgact acaaccgccg tgtgccgctg
    14761 ggcaatgcca acctgattgg tcgcgaagag ctgcaacacg cccgcgacgc cgtcaccagt
    14821 gcccaggcgc aactggacgt cgctattcaa caatacaatg ccaatcaggc gatgattctg
    14881 gggactaaac tggaagatca gcctgccgtg caacaggctg ccaccgaagt acgtaacgcc
    14941 tagctggcgc tggagcgtac ccgtattgtc agtccgatga ccggttatgt ttcccgtcgt
    15001 gccgtgcaac cgggtgcgca aattagccca acgacgccgc tgatggcggt cgttccagcc
    15061 accaatatgt gggtggatgc caactttaaa gagacgcaga ttgccaatat gcgcatcggt
    15121 cagccggtca ccatcaccac cgatatttac ggcgatgatg tgaaatacac cggtaaagtg
    15181 gttggtctgg atatgggcac aggtagcgcg ttctcactgc ttccggcgca aaatgcgacc
    15241 ggtaactgga tcaaagtcgt tcagcgtcta cctgtgcgta tcgaactgga ccagaaacag
    15301 ctggagcaat acccgctgcg cattggtttg tccacgctgg tgagcgtcaa taccactaac
    15361 cgtgacggtc aggtactggc aaataaagta cgttcaacac cggtcgcggt aagtaccgcg
    15421 cgtgaaatca gcctggcacc tgtcaataaa ctgatcgacg atatcgtaaa agctaacgct
    15481 ggctaatcca gaggtgcgtg tgatgcaaca gcaaaaaccg ctggaaggcg cgcaactggt
    15541 cattatgacg attgcgctgt cactggcgac attcatgcag gtgctgaact ccaccattgc
    15601 taacgtggcg atccccacta tcgccgggaa tctgggctca tcgctcagcc aaggaacgtg
    15661 ggtaatcact tctttcgggg tggcgaatgc catctcgatc ccgcttaccg gctggctggc
    15721 aaagcgcgtc ggggaagtga aactgttcct ttggtccacc atcgcctttg ctattgcgtc
    15781 ttgggcgtgt ggtgtctcca gcagcctgaa tatgctgatc ttcttccgcg tgattcaggg
    15841 gattgtcgcc gggccgttga tcccgctttc gcaaagtcta ttgctgaata actacccgcc
    15901 agccaaacgc tcgatcgcgc tggcgttgtg gtcgatgacg gtgattgtcg cgccaatttg
    15961 cggcccgatc ctcggcggtt atatcagcga taattaccac tggggctgga tattcttcat
    16021 caacgtgccg attggcgtgg cggtggtgtt gatgacactg caaaccctgc gcggacgtga
    16081 aacccgcacc gaacggcggc ggattgatgc cgtggggctg gcactgctgg ttattggtat
    16141 cggcagcctg cagattatgc tcgaccgcgg taaagagctg gactgttttt catcacagga
    16201 aattatcatt cttaccgtgg tggcggtggt ggctatctgc ttcctgattg tctgggagct
    16261 gaccgacgat aacccgatag tcgatctgtc gttgtttaag tcgcgcaact tcaccatcgg
    16321 ctgcttgtgt atcagcctcg cgtatatgct ctacttcggc gctattgttc tgctgccgca
    16381 gttgttgcag gaggtctacg gttacacggc gacctgggca ggtttggcct ctgcgccggt
    16441 agggattatt ccggtgattc tgtcgccgat tatcggccgc ttcgcgcata aactggatat
    16501 gcggcggctg gtaaccttca gctttattat gtatgccgtc tgcttctact ggcgtgccta
    16561 tacctttgaa ccaggtatgg atttttgcgc gtcggcctgg ccgcagttta tccaggggtt
    16621 tgcggtggtc tgcttcttta tgccgctgac caccattacg ctgtctggtt tgccaccgga
    16681 acgactggcg gcggcatcga gcctctctaa ctttacgcga acgctggcgg ggtctatcgg
    16741 cacgtcgata accacgacca tgtggaccaa ccgcgagtcg atgcaccatg cgcagttgac
    16801 tgagtcggta aacccgttca acccgaatgc ccaggcgatg tacagtcaac tggaagggct
    16861 tgggatgacg caacagcagg catcaggctg gattgcccag cagatcacca atcaggggct
    16921 gattatttcc gccaatgaga tcttctggat gtcagccggg atattcctcg tactgctggg
    16981 gctggtgtgg tttgctaaac cgccatttgg cgcaggtggc ggcggaggcg gtgcgcacta
    17041 agtacaacta agccagttca tttgaactgg cttttttcaa ttaattgtga agatagttta
    17101 ctgactagat gtgcagttcc tgcaacttct ctttcggcag tgccagttct tcgttgctgt
    17161 tgatgcgtac ctcacgttcc agaatgctac gcgcaatatc ctgcgcttcc tgcaacgagt
    17221 gcatctggta agtgccacac tggtagacgt tcagttccgg gatctgattc tgatcctgca
    17281 ctttcagcac gtcttccatt gccgctttcc aggcatcagc aacacgctgc tcatctggcg
    17341 taccaatcag actcatataa aaaccggtgc ggcagcccat tggcgagata tcgataatct
    17401 ctacaccatt accgttaaga tggttacgca taaaaccagc aaacaggtgc tccagggtat
    17461 ggatccctct ttctggcatc acttctttgt tcggcacgca gaagcgcaga tcgaacacgg
    17521 tgattgcgtc gccatgcggg gtgttcattg ttttcgccac ccgaactgca ggcgcttcca
    17581 tccgggtatg atcgactgtg aagctatcta acaacggcat ttagccacct ccggtaattt
    17641 ttttaaaaat tttctgaact ctttcttccc aggcgagtct gagtatatga aagacgcgca
    17701 tttgttatca tcatccctga attcagagat gaaattttgg ccactcccga gtggcctttt
    17761 tcttttctgt caggcgtgtt tttccagcca caccgcaaac ggttcggtat cagcggtttc
    17821 catttcctgc tgacggcgtt cagatgcctc gcgctcggct acaaaatcct cttcgcgcag
    17881 aatttccagc ggctcttcac gcagcagatt acggtaggct tctgcaaacg ctttgcctgt
    17941 tccgccaata ccagtatcaa tcatagacct taagatacgg gcagagaaag tcagatcggg
    18001 attatcgaag caggcaacca gttcatcaca cactttctga tacgcttcgc cgccgttaat
    18061 gctatccagc gtttgcgcga cgcgtttcag atcgcggaac agatctttac ccacctgcag
    18121 taacgggaac tgcgcggttt cgcagccgat acccagcgtc aggcccggtt tgcgaccttc
    18181 aaggatcacc cggttccagt taacgcgcgt acaggcaagt tcgctactgc tcatttccgg
    18241 tgcatcagcc agcgcacacc agaccataaa caggtcgagg aatcgcacct gctgttcatc
    18301 tacaccaatc ggcgagaacg ggttgatgtc cagcgaacgc acttcaatat attcaatgcc
    18361 gccacgtaac agcgcatcag aaggtgactc gccgctgcgg gtaacgcgtt ttggacgaat
    18421 cggcgcatac agttcgtttt caatctgcag cacgttgcta ttgatttgca gcctcttacc
    18481 gtctttctca ataccaatct tcgcgtactc ttccgatggc gttttgattg cctgtttaag
    18541 gcccgctacg tactcgtaaa gatcgttgaa ggtaatacca agattgcttt gcgatttatt
    18601 ggtatagccg agatcgctca aacgaagaga ggtcgcatac ggcaggtaat acataccgca
    18661 ctcggttttc tcaaacggca gcgacgttgg ttttccttgc aggaaagaag aacaaatcgc
    18721 cggagatgca ccaaacagat aaggaataac ccaaccgaaa cgatagtaat tgcggataac
    18781 gcggaaatag cccgcagaaa ttttctcttt ggcatcagcg cccgagatat caccgcactt
    18841 cgcttgccag aatgccattg gcaaagagaa attgtagtgc acgccggaaa tggtttgcat
    18901 cagcgcgccg tagcgatttt tcagcccttc acgatacagc gttttaaagc gtccggtgtt
    18961 agaagtgccg tactgtgcca gttcgatgtc ctgaccttct gcgatgtagc atggcatact
    19021 taacggccac atccgctcat cgcccatatt gcgcgccgta taacgatgca gatcgcgcat
    19081 aaaggtcagc atatgttcaa tatcaccatc cactggtgta atgaattcca gcaatgcttc
    19141 cgcaaaatcg gtagtaatcc atttgtgcgt cagtgcggaa cctaatgctt caggatgacc
    19201 tgttgttgcc agtgtgccat cagcattaac acgcaaagtt tcgcgctcca gcccacgctg
    19261 tatccccttt aacgcctgag gatgtttttc cagccaggcc agcgcctgtg atacgtccgg
    19321 gatcaaattg acctcccgcc tgtcaaaatc gttttaatta gcataactgt aatggtgacc
    19381 atatgtgcag gcctacaatt agtgccacca catcatgccc tgaacggtcg ctgctgcaac
    19441 tgcaacatag cgtaacgctt taccaaggca caaaaaaaag attactggcc cccaggagat
    19501 gcgcatccat cccgctaaca gacacagtaa atcgccaacc acgggcatcc agcttaataa
    19561 tagcgtgact gcaccatagc gtttcagcca gccggtagct ttctcttgcc agcgcgatgt
    19621 tttacgcaat ggaaagaaac gcccaaggat aacgttagtt aaccctccaa ggctattacc
    19681 cattgttgct gttaaaacta aaacccaggg atgactgatc ccggaaagca acattgccac
    19741 cagcacgact tcggagttgc cgggtaatag tgtagcgctg agaaaactac tggcaaacaa
    19801 cgagaaaagc gataacgctt cactcacagc aagcgaacat ccacagcgtc catgcctgct
    19861 gcacgggccg cctgaatacc gaaatcggca tcttcaaaga ccacacactg cgtcggttgc
    19921 acgcccatac gctgcgcgca caacaaaaat gtgtctggcg cgggtttatg gtgtttgacg
    19981 tgatcagcag cgacgacggc gtcaaaataa cggcgtaatc ccaggtgcgc cagcaatgcc
    20041 tcagcgatgg cgctttcact ccccgttcct acagccattg ggcgacgacc atgccaactt
    20101 ttcaccactt caacaagagg aagcggctcg acactatcca gcagcatact tcttactgct
    20161 tctgtttttt cacgcgctaa cgcatgcggg tcgagatcgg cctgattcag ctcaataatt
    20221 gcctgagcaa tacgccaggt gggcgatcca ttaagcgcaa tcatcgcctg aatatcgtac
    20281 tgaagaccgt agtgccctaa tacttcgcgc cacgctttac ggtgcgtagg cgccgtatcc
    20341 aggattgtgc catccatatc aaaaattaaa cctgcataac gctcgtacat ggtcttctcg
    20401 caagtcgaaa aatatgacgc tactttagcg taattgcttg attttgtcgc tgatggcgaa
    20461 ggagaagaaa attcaggaaa tggataaagt agttatttag aaatgagata tttttgaagg
    20521 aaattttttg gagaatatgg tgcatccggg aggattcgaa cctccgaccg ctcggttcgt
    20581 agccgagtac tctatccagc tgagctacgg at
//
LOCUS       DAEBXN010000075        20521 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.075, whole genome shotgun sequence.
ACCESSION   DAEBXN010000075 DAEBXN010000000
VERSION     DAEBXN010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20521
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.075"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            243..584
                     /gene="raiA"
                     /locus_tag="KJL99_002742"
     CDS             243..584
                     /gene="raiA"
                     /locus_tag="KJL99_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBD4437415.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    687..812
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            688..735
                     /gene="pheL"
                     /locus_tag="KJL99_002743"
     CDS             688..735
                     /gene="pheL"
                     /locus_tag="KJL99_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HBD4437416.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            834..1994
                     /gene="pheA"
                     /locus_tag="KJL99_002744"
     CDS             834..1994
                     /gene="pheA"
                     /locus_tag="KJL99_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HBD4437417.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="KJL99_002745"
     CDS             complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="KJL99_002745"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HBD4437418.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGKVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="KJL99_002746"
     CDS             complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="KJL99_002746"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HBD4437419.1"
                     /translation="MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4449..4814
                     /locus_tag="KJL99_002747"
     CDS             4449..4814
                     /locus_tag="KJL99_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HBD4437420.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKRADENASTIRLN"
     gene            4964..5482
                     /locus_tag="KJL99_002748"
     CDS             4964..5482
                     /locus_tag="KJL99_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HBD4437421.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDASSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5472..6697
                     /gene="dgcN"
                     /locus_tag="KJL99_002749"
                     /pseudo
     CDS             5472..6697
                     /gene="dgcN"
                     /locus_tag="KJL99_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            6713..7195
                     /locus_tag="KJL99_002750"
     CDS             6713..7195
                     /locus_tag="KJL99_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBD4437422.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVIATP
                     "
     gene            complement(7272..7619)
                     /gene="rplS"
                     /locus_tag="KJL99_002751"
     CDS             complement(7272..7619)
                     /gene="rplS"
                     /locus_tag="KJL99_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HBD4437423.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(7661..8428)
                     /gene="trmD"
                     /locus_tag="KJL99_002752"
     CDS             complement(7661..8428)
                     /gene="trmD"
                     /locus_tag="KJL99_002752"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HBD4437424.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(8459..9007)
                     /gene="rimM"
                     /locus_tag="KJL99_002753"
     CDS             complement(8459..9007)
                     /gene="rimM"
                     /locus_tag="KJL99_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HBD4437425.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(9026..9274)
                     /gene="rpsP"
                     /locus_tag="KJL99_002754"
     CDS             complement(9026..9274)
                     /gene="rpsP"
                     /locus_tag="KJL99_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HBD4437426.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(9411..10772)
                     /gene="ffh"
                     /locus_tag="KJL99_002755"
     CDS             complement(9411..10772)
                     /gene="ffh"
                     /locus_tag="KJL99_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HBD4437427.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGNFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            10939..11730
                     /locus_tag="KJL99_002756"
     CDS             10939..11730
                     /locus_tag="KJL99_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HBD4437428.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLVICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLMHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            11751..13037
                     /locus_tag="KJL99_002757"
     CDS             11751..13037
                     /locus_tag="KJL99_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBD4437429.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGKYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(13092..13685)
                     /gene="grpE"
                     /locus_tag="KJL99_002758"
     CDS             complement(13092..13685)
                     /gene="grpE"
                     /locus_tag="KJL99_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HBD4437430.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            13808..14686
                     /gene="nadK"
                     /locus_tag="KJL99_002759"
     CDS             13808..14686
                     /gene="nadK"
                     /locus_tag="KJL99_002759"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HBD4437431.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            14772..16433
                     /gene="recN"
                     /locus_tag="KJL99_002760"
     CDS             14772..16433
                     /gene="recN"
                     /locus_tag="KJL99_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HBD4437432.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKYDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            16582..16923
                     /gene="bamE"
                     /locus_tag="KJL99_002761"
     CDS             16582..16923
                     /gene="bamE"
                     /locus_tag="KJL99_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HBD4437433.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(16985..17275)
                     /locus_tag="KJL99_002762"
     CDS             complement(16985..17275)
                     /locus_tag="KJL99_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HBD4437434.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(17265..17702)
                     /gene="ratA"
                     /locus_tag="KJL99_002763"
     CDS             complement(17265..17702)
                     /gene="ratA"
                     /locus_tag="KJL99_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HBD4437435.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            17873..18355
                     /gene="smpB"
                     /locus_tag="KJL99_002764"
     CDS             17873..18355
                     /gene="smpB"
                     /locus_tag="KJL99_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HBD4437436.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            18570..18932
                     /gene="ssrA"
                     /locus_tag="KJL99_002765"
     tmRNA           18570..18932
                     /gene="ssrA"
                     /locus_tag="KJL99_002765"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            19137..20378
                     /locus_tag="KJL99_002766"
     CDS             19137..20378
                     /locus_tag="KJL99_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBD4437437.1"
                     /translation="MARQTKPLSVKEIESAKPKEADYVLYDGDGLELLIKSSGSKIWQ
                     FRYIRPVTKTRAKKSIGPYPSVTLADARNYRAESRSLLAKQIDPQEHQQEQLRSSLEA
                     KTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQ
                     PVQARGALETVRRLCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LSQLMQTMRTASISLSTRCLFMWQLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKM
                     NRDHTVPLSDEAIAVLEMMKPLSGNREFIFPSRIKPNQLMNSQTVNASLKRAGFGGVL
                     VSHGLRSIASTALNEQGFPPDAIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWANF
                     VMAADRGSMIEGGIKGMKLVG"
BASE COUNT         5300 a         5357 c         4815 g         5049 t
ORIGIN      
        1 cttgagctgc cgttttttta ttctgttagt tgtgaaactg aagcgattta gtcactatcg
       61 atctcatcaa atatggctcg ctttgagata ttcctcaagt aaaaaaacac ctcttcctgc
      121 gatttctcac aaaaaagatt cgttgacaaa aagtgacaaa attatgagat ttccatcaca
      181 cattttgaca tcaggaacgg tatgctgaat tcaccaagac gggaagacaa gaggtaaaat
      241 ttatgacaat gaacattacc agcaaacaaa tggaaattac tccggccatc cgccaacatg
      301 tcgcagaccg tctcgccaaa ctggaaaaat ggcaaacaca tctgattaat ccacatatca
      361 ttctgtccaa agagccacaa gggtttgttg ctgacgccac aatcaataca cctaacggcg
      421 ttctggttgc cagtggtaaa catgaagata tgtacaccgc aattaacgaa ttgatcaaca
      481 agctggaacg gcagctcaat aaactgcagc acaaaggcga agcacgtcgt gccgcaacat
      541 cggtgaaaga cgccaacttc gtcgaagaag ttgaagaaga gtagtccttt atattgagtg
      601 tatcgccaac gcgccttcgg gcgcgttttt tgttgacagc gtgaaaacag tacgggtact
      661 gtactaaagt cacttaagga aacaaacatg aaacacatac cgtttttctt cgcattcttt
      721 tttaccttcc cctgaatggg aggcgtttcg tcgtgtgaaa cagaatgcga agacgaacaa
      781 taaggcctcc caaatcgggg ggcctttttt attgataaca aaaaggcaac actatgacat
      841 cggaaaaccc gttactggcg ctgcgagaga aaatcagcgc gctggatgaa aaattattag
      901 cgttactggc agaacggcgc gaactggccg tcgaggtggg aaaagccaaa ctgctctcgc
      961 atcgcccggt acgtgatatt gatcgtgaac gcgatttgct ggaaagatta attacgctcg
     1021 gtaaagcgca ccatctggac gcccattaca ttactcgcct gttccagctc atcattgaag
     1081 attccgtatt aactcagcag gctttgctcc aacaacatct caataaaatt aatccgcact
     1141 cagcacgcat cgcttttctc ggccccaaag gttcttattc ccatcttgcg gcgcgccagt
     1201 atgctgcccg tcactttgag caattcattg aaagtggctg cgccaaattt gccgatattt
     1261 ttaatcaggt ggaaaccggc caggccgact atgccgtcgt accgattgaa aataccagct
     1321 ccggtgccat aaacgacgtt tacgatctgc tgcaacatac cagcttgtcg attgttggcg
     1381 agatgacgtt aactatcgac cattgtttgt tggtctccgg cactactgat ttatccacca
     1441 tcaatacggt ctacagccat ccgcagccat tccagcaatg cagcaaattc cttaatcgtt
     1501 atccgcactg gaagattgaa tataccgaaa gtacgtctgc ggcaatggaa aaggttgcac
     1561 aggcaaaatc accgcatgtt gctgcgttgg gaagcgaagc tggcggcact ttgtacggtt
     1621 tgcaggtact ggagcgtatt gaagcaaatc agcgacaaaa cttcacccga tttgtggtgt
     1681 tggcgcgtaa agccattaac gtgtctgatc aggttccggc gaaaaccacg ttgttaatgg
     1741 cgaccgggca acaagccggt gcgttggttg aagcgttgct ggtactgcgc aaccacaatc
     1801 tgattatgac ccgtctggaa tcacgcccga ttcacggtaa tccatgggaa gagatgttct
     1861 atctggatat tcaggctaat cttgaatcag cggaaatgca aaaagcattg aaagagttag
     1921 gggaaatcac ccgttcaatg aaggtattgg gctgttaccc aagtgagaac gtagtgcctg
     1981 ttgatccaac ctgatgaaaa ggtgccggat gatgtgaatc atccggcact ggattattac
     2041 tggcgattgt cattcgcctg acgcaataac acgcggcttt cactctgaaa acgctgtgcg
     2101 taatcgccga accagtgctc caccttgcgg aaactgtcaa taaacgcctg cttatcgccc
     2161 tgctccagca actcaatcgc ctcgccgaaa cgcttatagt aacgtttgat taacgccaga
     2221 ttacgctctg acgacataat gatgtcggca taaagctgcg gatcctgagc aaacagtcgc
     2281 ccgaccatcg ccagctcaag gcggtaaatc ggcgaagaga gcgccagaag ttgctcaagc
     2341 tgaacatttt cttctgccag gtgcagcccg taagcaaaag tagcaaagtg gcgcagagcc
     2401 tgaataaacg ccatattctg atcgtgctcg acagcgctaa tacgatgcaa ccgagcgccc
     2461 cagacctgaa tttgctccag aaaccattgg tatgcttccg gcttacgtcc atcacaccag
     2521 accacaactt gctttgccag gctaccgctg tccgggccga acatcgggtg taaccccagt
     2581 accgggccat cgtgcgccgc cagcatggcc tgtaatggtc catttttcac tgatgccaga
     2641 tcaaccagaa tacaatcttt cggtaaaggc ggtaatttgc cgataacttg ctcagtaacg
     2701 tggattggca cactaacaat caccattccg gcatcggcaa caatatcagc cgctcgatcc
     2761 cagtcatgtt gctccagaat ccgcacctga taacccgata gtgtcagcat cttctcgaac
     2821 aggcgtccca tctgaccgcc accgccgacg ataaccaccg gacgcagtga cggacaaagt
     2881 gttttaaatc ctttgtcgtt ttcactggag taagattcac gcatcacccg acgcaaaaca
     2941 tcctcaatca gatctggcgg tacacccaga gcttccgcct ctgcacgacg cgaggccaac
     3001 atagatgcct cgcgctccgg aacataaata ggcagtccaa agcggctttt caccttgccc
     3061 acttcagcaa ccagttccag acgcttcgct aataaattca gcagcgcttt atcgacttca
     3121 tcaatttgat cgcgtaatgc ggtcaattca gcaaccataa taaacctctt aagccacgcg
     3181 agccgtcagc tgcccgttca gatcctgatg aatttcacgc agcaaggcat cggtcatttc
     3241 ccagctaatg caggcatcgg ttacggatac accgtatttc atttcactgc gcggttgctc
     3301 agaagactga ttgccctcgt ggatattact ttcgatcatc agaccaataa ttgagcgatt
     3361 accatctttg atttgagcaa ccacggattc tgccaccgca ggctgacggc gataatcttt
     3421 attggaatta ccgtggctgc aatctaccat cagagacggg cgcagtcccg cctgttccat
     3481 ctctttttca cattgcgcaa catccgcagg gctatagttc ggcgctttac caccgcgcag
     3541 gatcacatgc ccgtccggat tcccctgagt ttgtagcaac gcaacctgcc ctgcctggtt
     3601 aatgccaaca aaacagtgcg gctgggcggc ggcgcgcata gcgttaattg ctgttgccag
     3661 actgccgtcg gtgccgtttt taaaaccaac cggcatggaa agcccggagg ccatttcacg
     3721 gtgagtttgc gattccgttg tacgagcacc aattgctgac cagctaaaca gatcgcccag
     3781 gtattgcggg ctattcggat ctaacgcttc cgtcgccagt ggcagtccca tattcaccag
     3841 ctcaagcaac aatttacgcg cgatttgcag cccggcttct acatcaaaag agccatccat
     3901 atggggatcg ttaattaacc ctttccagcc gacagtggta cggggttttt caaaatagac
     3961 gcgcattacc agatagaggc tatcgctgac ctctgcggca agagctttaa atcgacgagc
     4021 atattccaga gcagtttccg gatcatgaat ggaacaagga ccacatacta ccagcagacg
     4081 aggatcgcgc ccggcgataa tatctgaaat ggttttacgc gagtcagcaa tctgggcttc
     4141 ttgttgcagg ctcaatggaa aagcggcctt cagttgttcc ggagtcatta aaacctgttc
     4201 gtcggtaata tgtacgttat tcagcgcgtc tttatgcatg atggcgatcc tgtttatgct
     4261 cgtttgcgat agttgctcct cagcgaggat gacgtaacga taacacataa agtaaagttt
     4321 tcaatccata tttcgtacat ttttatttac acaggcaatt tagtcgcgct ttcaaccctt
     4381 gcttctgtat agataaattt acaccccctt taaaaacaat ccgctatgct ttgaaaaagg
     4441 agaaagaaat gatgaaaaag tttatcgccc ccttgttggc tttactggtt agcggatgtc
     4501 agattgatcc ttatactcac gcgccaacct tgaccagcac cgactggtat gatgtcggta
     4561 tggaagatgc gatatcgggc agcgccataa aagatgacga tgcatttagc gattcacagg
     4621 cggatcgcgg tctatacctt aaaggatatg ccgaagggca aaagaaaact tgccagaccg
     4681 attttactta tgcccgggga ctttccggta aaagctttcc tgccagctgc aacaatgttg
     4741 aaaatgccag ccaactgcat gaagtatggc aaaaaagggc tgatgaaaat gccagcacaa
     4801 tacgattaaa ttaattaaaa ccccacaaat aaattaaaca taagatttta ctcattgtgt
     4861 tttatttctc acattgatga cggtcgcatg aaaaaatgat attttaatga gcatcctgaa
     4921 aaaatatcga tgcatttcga gcgaagatgg tggggatccc tgaatgcgtt tttctcaccg
     4981 actgttctta cttctgattc tcctgctgac gggagcacca atactcgctc aagagccttc
     5041 tgacgttgcg aaaaacgtgc gtatgatggt ttccggtatt gtcagttaca cccgctggcc
     5101 tgcgctatca gggccaccaa agttatgtat cttttcatct tcacgtttta gtaccgccct
     5161 acaagagaat gccgcgacat ctttgcctta tctgcctgtc atcattcaca ctcaacaaga
     5221 ggcgatgatt tcaggctgca atggttttta ttttggcaac gagtcaccga cattccagat
     5281 ggaattaacc gaacaatatc cgtcaaaggc gttattatta attgccgaac aaaataccga
     5341 gtgcattatt ggtagcgcct tttgtctgat catccacaat aatgacgtca gatttgccgt
     5401 aaacctggat gcctcatcgc gtagcggtgt aaaggtcaac ccggatgtct taatgctcgc
     5461 acggaagaaa aatgatggat aacgataatt ctcttaataa gcgccccacg tttaaaagag
     5521 cattacgcaa catcagtatg accagcatat ttatcactat gatgctgatc tggttgctgc
     5581 tttccgtgac ctcggtgctg accctgaaac agtacgcgca aaaaaacctg gcactgacag
     5641 cagcaacaat gacttacagt ctggaagcag ctgtcgtttt tgccgatggc cctgcagcaa
     5701 ctgaaacact ggcagcgctg ggccagcaag ggcattttca actgcagaag tacgtgataa
     5761 gcagcaaaat attctggcat cctggcatta cccccgtaag gatccaggcg atactttcag
     5821 caatttcata agccactggc tcttccctgc ccccatcatt cagccgattc gtcacaatgg
     5881 tgaaaccatt ggcgaagtac gcttaaccgc tcgcgacagt tcaatcagcc attttatctg
     5941 gttttcgctc gccgtactga ccggttgtat tctgctggca tcaggcatcg caattaccct
     6001 cacccgccat ttgcacaatg gcctggtgga agcactgaaa aatatcaccg atgtcgtaca
     6061 tgatgtgcgt tccaaccgca atttttcccg acgagtttcg gaagaacgta tcgctgagtt
     6121 tcaccgcttc gctctcgact tcaacagtct gctggatgaa atggaagagt ggcagcttcg
     6181 tttacaggcc aaaaatgcgc agcttctgcg taccgcgcta catgacccat taaccgggct
     6241 ggctaaccgc gcagcgtttc gtagcggcat caatacgttg atgaacaatt ccgatgcccg
     6301 aaaaacgtcg gcgttactat ttcttgatgg cgataatttc aaatatatca atgatacctg
     6361 gggtcatgcg acgggcgata gggtcttgat tgaaatcgca aaacggttag ctgaatttgg
     6421 cgggctgcga cataaagcat accgcctggg cggcgatgaa ttcgctatgg tgctctatga
     6481 tgtacagtcg gaatctgaag tgcagcagat atgctcagca ctgacacaaa tctttaatct
     6541 cccgtttgat cttcataatg gccatcagac caccatgaca ttaagcattg gttacgcgat
     6601 gaccattgag cacgcctctg cggaaaaatt acaagagctt gccgatcaca atatgtatca
     6661 ggccaaacac cagcgtgccg aaaagctggt gagataacaa ggatatatcg atatgataaa
     6721 gcacctggta gcacccctga ttttcacctc actaatactg actggctgcc agtcccctca
     6781 gggaaagttt actcctgagc aagtcgccgc tatgcaatct tatggattta ctgaatccgc
     6841 cggcgactgg tcgctgggct tatcagatgc cattctgttc gcaaaaaatg actacaaatt
     6901 gctcccggaa agccagcaac agatccaaac catggcagcc aaattggcct cgacagggct
     6961 aacacatgcc cgtatggatg gacacaccga taactatggt gaagacagtt acaacgaagg
     7021 cttatcattg aaacgggcga atgtcgtggc cgatgcatgg gctattggtg gacaaattcc
     7081 acgcagcaat ctcaccacac agggtttagg aaaaaaatat cccatagcca gtaacaagac
     7141 cgcccagggc cgcgccgaga accgccgcgt cgcagtggtg attgcaaccc cttaaaacac
     7201 gagcaaacag acaaaaaaag ggccagccaa ttggccagcc cttcttaaca ggatgtcgct
     7261 taagcgaaat cttagttaag acgctctttg atacgagcag ccttaccagt acgctcacgc
     7321 aggtagtaca gtttagcttt acgaacagca ccacgacgtt tgacagaaat gctgtcaact
     7381 accggagagt gagtctggaa gacacgctca acgccttcgc cgttggaaat tttacgaaca
     7441 gtgaatgcag agtgcagacc gcggttacga atagcgataa ccacgccctc gaatgcctgc
     7501 agacgttttt tggaaccttc aacaacccat actttcactt ccacggtatc acccggacgg
     7561 aaggaaggta cgtcctgctt catctgctct tgttcaagtt gcttaataat gttgctcata
     7621 atttaatctc ttatcctggg taaactgatc tctcgggggc ttacgccatc ccatcatgtt
     7681 tatgttgctg ttgtgcgtgt tccgttttga actccgccag caaccttgct tgctcttcag
     7741 tcagagccag gttttccaga agttcaggtc ttctaagcca ggtacggccc agcgactgtt
     7801 tcaaacgcca gcgacgtatc tcggcatggt tgcccgacag taacactggc ggaacttcca
     7861 tcccttctaa cacctcaggc cgcgtatagt gcgggcaatc cagcaatcct tcagcaaagg
     7921 aatcttccgt tgccgaggct tcatgtccca gtactcccgg aataaaccgg gaaacggagt
     7981 caatcagcgt cattgctggt aactcaccac cactgagaac gtaatcgccg attgaccatt
     8041 cttcgtcaat ttcggtttgg atcacgcgct catctatacc ttcgtagcga ccgcacacca
     8101 gaatcaattt ttgattcgtt gccagttcgc tgacgcccgc ttgatcaagc ttgcgtccct
     8161 gtggtgacag ataaatcacc tttgcgcctt cacccgccgc ggcttttgct gcatgaatgg
     8221 cgtcccgcaa gggttgcacc atcattaaca tccccggtcc gccgccgtaa ggacgatcgt
     8281 ccacggtacg gtgccggtca tgcgtgaagt cgcgaggact ccaactctgg atgctcagca
     8341 ggccattttt aactgcccgg ccagttaccc cgtaatcggt aattgcgcgg aacatttcag
     8401 gaaacaggct aattatgcca atccacatag cgccgccttt taccgtttat ccggtggttt
     8461 aaaaaccagg atcccaatct acttcgattg aacgagtagt gagatcgact ttcttgataa
     8521 cctgcccatc gaggaacggt acgagacgtt ccttgatacc aaacgcatct ttcaggtttg
     8581 ccttaatgac gagaacgtca ttagatccgg tttccatcat atcaacgact ttaccgagat
     8641 cgtagccttc agtggttact acctggcagc ccatcaggtc tttccagtag tagtccccct
     8701 cttcaagctg aggcagctgc gatgaatcca cgacaatttc acaattcgtc agcaggttcg
     8761 ccgcatcacg atcgtcaacg cctttcagct tgatgatcat gtcctgattg tggtgcttcc
     8821 agctttccag ctggacttgc tgccactgac ccgccttctg gataaaccag ggctgatagt
     8881 caaaaatgct ttcggcgtct tcggtggaag aaaacactct gagccaccca cgaataccgt
     8941 aagacgaacc catttttccc aaaacgatgg gatcaacagg tgcttgcgcg gtgagttgtt
     9001 tgctcatcat gaccaccgtg acagattaag ctgctttgtt tacttctttg atcagcgcag
     9061 caacgcgatc agaaatagtt gcgccctggc caacccagtg agcgatgcga tccagatcca
     9121 ggcgagtgcc ttcttctttt tcgctagcga ttgggttgaa gaaaccaacg cgctcgatga
     9181 agcgaccgtt gcgtgcatta cggctgtcag cgacaacaac ctggtagaac ggacgctttt
     9241 tagcgccgtg acgtgctaaa cgaatagtta ccataacatc ctcttgtgtg aataaaacaa
     9301 ccggacccca tcgaggaacg gagtccggtg tcatattaaa agcccgaaaa ttttactgat
     9361 ttctggagaa aatgcaatca gcagttaata actctgtcgc ataaggtcat ttagcgacca
     9421 ggaaatcccg gcggcatcat tcccttcatg cttctcatca tcttcgccat tccgcctttc
     9481 ttcattttct tcatcatgcg ctgcatgtcg tcgaactgct tcagaaggcg gttaacgtcc
     9541 tgtacctgca tcccgcaacc ggcggcgata cgacgcttac gcgaaccttt gatgatttct
     9601 ggcttagcgc gctctttcat cgtcattgag ttgatgatgg cttccatacg caccagcact
     9661 ttatcgtcca tctgcgactt gacgttatcc gggatctgcc ccatgcccgg cagtttgccc
     9721 atcaagctgg ccatgccgcc catatttttc atctggcgca gttgctcaag aaagtcgttg
     9781 agatcgaagc cgtcgccttt ttttagcttg ctggctaatt tctctgcctg cgcgcggtca
     9841 actttgcttt cgatatcttc gatcagcgac agtacgtcgc ccatgccgag aatacgcgac
     9901 gcgatgcggt ccggatggaa cggctccagc gcctcagttt tctcgccaac accgaggaac
     9961 ttgatcggtt tgccagtgat gtgacgaata gagagcgccg caccgccgcg ggcatcgccg
    10021 tccactttgg tcaacactac gccggtaagc ggtaacgctt cattgaatgc ttttgccgta
    10081 ttggccgcat cctgaccggt catggcgtca accacaaaca gggtttcaac cgggttaatc
    10141 gacgcatgga cttgtttgat ctcgtccatc atcgcttcgt caacgtgcag acgaccagcg
    10201 gtatccacca gcagcacgtc gtagaatttc agtttggctt ctttcagcgc cgcgttaaca
    10261 atatcaaccg gcttctgacc aacatcagaa gggaagaaat caacgcccac ctgctctgcc
    10321 agcgtctcaa gctgtttgat tgccgccggg cgatagacgt cagcagaaac caccagcact
    10381 ttcttcttgt gcttctcgcg caggaaatta ccgagtttac caacgctggt tgttttaccg
    10441 gcaccttgca ggcctgccat cagcacgacc gcaggcggtt gcgcagccag attcagggtc
    10501 tggttctctt cgcccatcgc cgcaaccagt tcgttacgga cgattttgac gaactcctgc
    10561 cccggcgtca ggctcttatt aacttcatga ccaaccgctt tctcttttac gcgattgata
    10621 aactcacgca ctaccggcag agcgacgtcc gcctccagca gcgccatgcg cacttcgcgc
    10681 agcgtatctt ttacgttgtc ttcagtgagg cgtccacggc cactgatatt gcgcagcgtg
    10741 cgcgacaaac gatcggttaa attatcaaac attgtctctc gcctggggtg gaaacggttg
    10801 gccgcaatcg cgacacatca tcagtatttt gccgcagtat aacatgaagg cgtctttgtt
    10861 gttatgcaac ggttggagca gcgttcacct gacgctatac tgcttctctt tcttattgct
    10921 caaactgtcg acattactat gcccgttttt gctctgctcg cgcttgtcgc ctactccgtc
    10981 agtcttgcgc tgattgttcc cggtctgctg caaaaaaacg gcggctggcg gcgcatggct
    11041 attatttctg cggtcattgc gctggtctgc cacgcaatcg ctctggaagc ccgcatcctg
    11101 cccgacggtg atagcggaca aaacctcagc ctgctgaacg ttggttcatt ggtcagtttg
    11161 gtgatctgta cggtaatgac cattgtggct tctcgcaatc gtggctggtt gctgctaccc
    11221 attgtctatg cctttgcgct tatcaacctg gcgctggcga ccttcatgcc caatgaatac
    11281 atcacccatc tggaagcaac gcctgggatg ctgatgcaca ttggcttatc gctcttttcc
    11341 tatgccacgc taattatcgc cgccctgtac gcgctgcaac tggcgtggat tgattaccaa
    11401 ctgaagaaca agaagctggc atttaaccaa gaaatgccgc cattgatgag tatcgagcgt
    11461 aaaatgttcc acatcacgca gattggcgtg gtgctgctaa cgctcacgct ttgcactggc
    11521 ctgttctaca tgcacaacct atttagcatg gaaaatatcg acaaggctgt gctctctatc
    11581 gtggcgtggt ttgtctatat tgtgctgctg tggggacatt accatgaagg atggcgtgga
    11641 cgccgcgtcg tctggtttaa cgttgcgggc gcggtaattc tgacactggc ctacttcggc
    11701 agccgaattg tccagcagtt aatcagctaa acccagaaaa ggagtttccc ctggaacaca
    11761 tttctactac tacgttgatc attattctga tcatcatggt ggtcatttca gcctattttt
    11821 ccgggtccga aaccggaatg atgaccctca accgctatcg tcttcgacat atggcgaaac
    11881 agggtaatcg ctcggccaaa cgcgtcgaaa aattgctgcg taagccagac cgcctgataa
    11941 gcctggtgtt aatcggcaat aacctggtca atattcttgc ctccgcgctc ggcactattg
    12001 tcgggatgcg tttgtacggc gatgcgggcg tggcaattgc gactggtgtg ctgacttttg
    12061 tcgtactggt ttttgctgag gtattgccga aaaccattgc cgcgctgtac ccggaaaaag
    12121 tcgcttatcc gagtagtttt ctgctggctc cgctgcaaat tttgatgatg ccgctggtct
    12181 ggttgctgaa tgctatcacc cgtatgctga tgcgcatgat gggtatcaaa accgatatcg
    12241 tggttagcgg ctctttgagc aaagaagagt tgcgcactat cgtgcatgaa tcgcgctcac
    12301 aaatttcccg tcgcaatcag gatatgctgc tgtcggtgct cgatctggaa aaaatgaccg
    12361 ttgatgacat catggtaccg cgcagtgaaa ttatcggtat tgatatcaac gatgactgga
    12421 aatctattct gcgccaactc tcccactcac ctcacgggcg catcgtgctt taccgtgatt
    12481 cgctggacga cgccatcagt atgctgcgag tacgtgaagc ctggcggttg atgtcggaga
    12541 aaaaagagtt caccaaagaa accatgctgc gcgccgcgga cgagatctat tttgtgcctg
    12601 aaggtacgcc gctcagcacg cagttggtaa agtttcagcg caacaaaaag aaagtcggcc
    12661 tggtcgtcaa cgagtatgga gacattcagg ggctggtgac ggttgaagat attctggaag
    12721 agattgttgg cgatttcact acgtcgatgt cgccaacact tgccgaagag gtcacgccgc
    12781 aaaacgacgg ttcggtgatt atcgatggca ccgccaacgt gcgggaaatc aacaaagcct
    12841 ttaactggca tctaccggaa gatgatgccc gcacggttaa tggcgtcatt cttgaggcac
    12901 tggaagagat ccctgtcgca ggcacccgcg tgcgtattgg caagtacgat atcgatattc
    12961 tcgacgtaca ggacaatatg attaagcagg taaaagtttt tcctgtgaaa ccgctgcgcg
    13021 agagtgtggc ggagtaacga aaacggccct gcattcgcat gcagggccgt gaattattac
    13081 gaaaacagaa attacgcttt tgctttcgct acagttacca tcgccgcacg aatcgtacga
    13141 ccattcagcg tataaccctt ctgcataatg cccagtacgt tacctggcgc aacgtcatca
    13201 gattccacca ttgcgatagc ctgatgcaca ttcgggtcca gcggcacgtt agtttcggcg
    13261 atcacttcaa caccaaactt acgcacaaca tccagcatcg acttcagcgt cagctcaatg
    13321 ccttcaacca tcgcagacat atccgggtta gctttatcag ccacttccag cgcacgatcc
    13381 aggctatcaa tcaccggcag caattcgttg atgaatttct ccagcgcgaa tttgtgggct
    13441 ttttcaatat ccagttcagt acgacgacgc aggttttcca tttcggcttt tacgcgcaaa
    13501 ataccgtcac gttcacgggt ctgggcttca gccagctgag cttcgagatt cgcaattttt
    13561 tcatcgcgcg gatccacctg ctcagcagaa gcttctggct caactgcctc aatctcttcg
    13621 tgctgatcca tgataatttc ttccggggct tgcccctcag gcgttttctg ttctttacta
    13681 ctcatgaatt tctccgcgtt tttttcgcat tcatctcgct aacttcgctt attatgggga
    13741 tcagtttcag ggtttcaagg gaagcactca cattgtcatc aatcttcgca acaaggacct
    13801 cggaaaaatg aataatcatt tcaagtgtat tggcattgtg ggacacccac ggcaccctac
    13861 tgcactgaca acacatgaaa tgctctaccg ctggctgtgc acaaaaggtt acgaggtcat
    13921 cgttgagcaa caaatcgctc acgaactgca actgaagaat gtgaaaactg gcacgctcgc
    13981 ggagattggg caactggctg atctcgcggt agtcgttggt ggcgacggta atatgctggg
    14041 cgcggcgcgc acgctcgccc gttacgatat taaagttatt ggaatcaacc gtggcaacct
    14101 gggtttcctg actgaccttg accccgataa cgcccagcaa caattagccg atgtgctgga
    14161 aggtcactac atcagcgaga aacgtttttt gctggaagcg caagtctgtc agcaagattg
    14221 ccaaaaacgc atcagcaccg cgattaacga agtggtactt catcctggca aagtggcgca
    14281 tatgatagag ttcgaagtgt atatcgacga gatctttgcg ttttctcagc gatctgatgg
    14341 gctaattatt tcgacgccaa caggctccac cgcctattcc ctctctgcgg gcggtcctat
    14401 tctgaccccc tctctggatg cgattaccct ggtgcccatg ttcccgcata cgttgtcagc
    14461 acgaccactg gtcataaaca gcagcagcac gatccgtctg cgtttttcgc atcgccgtaa
    14521 cgacctggaa atcagttgcg acagccagat agcactgccg attcaggaag gtgaagatgt
    14581 cctgattcgt cgctgtgatt accatctgaa tctgattcat ccgaaagatt acagttattt
    14641 caatacatta agcaccaagc tcggctggtc aaaaaaatta ttctaatttt acgccagcct
    14701 ctttactgta tataaaacca gtttatactg tacacaataa cagtaatggt ttttcataca
    14761 ggaaaacgac tatgttggca caactgacca tcagcaactt tgctatcgtt cgtgagcttg
    14821 agattgattt tcatagcggc atgaccgtaa taactggcga gaccggtgcg ggtaaatcta
    14881 ttgcaataga tgccctcggt ctttgtctcg gtggtcgcgc tgaagccgac atggtgcgta
    14941 ccggcgctgc tcgcgctgac ctgtgcgccc gtttttctct gaaagatacg ccagcggccc
    15001 tgcgctggct ggaagaaaac cagcttgaag acgggcatga atgtttgctt cgtcgcgtga
    15061 tcagcagtga tggtcgctcc cgtggtttca tcaacggtac agctgttcct ctgtcacaac
    15121 tgcgcgaact gggtcagttg ctgattcaga tccatggtca gcacgctcat caattactca
    15181 ccaaacctga gcaccaaaaa ttcctgcttg atggctatgc caatgaaacc tctctacttc
    15241 aggaaatgac cgcacgttat cagttgtggc atcaaagctg ccgtgacctc gcgcatcatc
    15301 aacagttaag tcaggaacgc gccgcccgtg cggaactgct gcaataccaa ttaaaagaac
    15361 ttaacgaatt taatccgcag cccggagagt ttgagcaaat cgacgaagag tacaaacgtc
    15421 tggcgaacag cggtcaattg ctgaccacca gccagaatgc attggcatta atggccgacg
    15481 gtgaagacgc aaacctgcaa agtcagcttt acacggctaa acaactggtg agcgaattga
    15541 ttggcatgga cagcaaactg tccggcgtac ttgatatgct ggaagaagct accatccaga
    15601 ttgctgaagc cagcgacgaa ctgcgccact actgcgatcg tctggatctc gatcccaacc
    15661 gactgtttga acttgaacag cgcatctcaa aacagatttc gctggcacgt aaacatcacg
    15721 tcagccctga ggcattacca cagtattacc agtcgctact ggaagaacag cagcaactgg
    15781 acgatcaggc cgactcacaa gaaacgctcg cgctggcggt aacgaaacat catcagcagg
    15841 cactggaaac cgcgcgcgca ttacaccaac aacgccagca atatgcagaa gaacttgcac
    15901 agctgatcac cgacagtatg catgcgctct caatgccgca tgggcagttt acgattgatg
    15961 tcaaatatga cgagcatcac ctgggcgctg acggtgccga tcgtattgag tttcgggtaa
    16021 ccaccaaccc aggtcagcca atgcagccta ttgccaaagt cgcatccggt ggtgaattgt
    16081 cccgcatcgc actggcaatc caggtcatca cggcgcgtaa aatggaaacc ccggcactga
    16141 tttttgatga agtggatgta gggattagcg gtccaacagc ggcagttgtc ggcaaactgc
    16201 tgcgtcaact cggcgaatca actcaggtga tgtgtgttac ccacctgcca caagtcgcgg
    16261 gatgtggtca tcaacactat tttgtcagca aagaaaccga tggtgcgatg acagaaacac
    16321 atatgcaatc tctggataaa aaagcgcggt tacaagagct ggcgcgcctg cttggtggca
    16381 gtgaagtcac acgtaacaca ctggcgaatg cgaaagaact gcttgcagcg taaacttttt
    16441 tcctgcttca cggtcagagt aaacagcaaa acgccgtaag accggaaagc aaaaggtttt
    16501 aaagtgatga aaggtctatt atcatcggca tattacagat gagccacgta ctgctcgggc
    16561 ccgaaaagga atcaaatcac tatgcgctgt aaaacgctga ctgctgcagc agcagtacta
    16621 ttgatgttaa ccgcaggctg ttccactctg gagcgagtgg tttaccgtcc tgacatcaac
    16681 caggggaact atctgaccgc taacgacgta tccaaaatac gtgttggcat gacgcaacaa
    16741 caagttgcgt acgcattggg tacaccgctg atgtccgatc catttggtac gaatacctgg
    16801 ttctatgtct tccgccagca accaggtcat gaaggtgtaa cgcagcaaac gctgacgctg
    16861 acctttaaca gtagcggtgt gttgaccaat attgataaca aacctgcgct gagtggtaac
    16921 taataataaa gttgctctca aagacgttaa aaaaggtgct caatgagcac cttttttctg
    16981 tctgttattt attcgctgat ttttctgctc gttgcctgcg aagctctttc ggatcggcaa
    17041 tgagagggcg ataaatctcc acccgatcgc catcatgcac actatcgctt agttttgccg
    17101 gacggctgta aatgccgact ttgtttttag ttaaatcgat atcggtacgc aattccagca
    17161 agccactggc gcgaatagct tcttcaaccg tcgcgccctc ctgcagcgtc actcgctgca
    17221 ggtattgctt ctcaggtagc gcataagcca cctcaacggc aattttacct agcactgtaa
    17281 acctctttcg cacgaaccgt aaaagcctgg accatattag ccgccagctc tttaaacacg
    17341 cgaccaaagg cgagttcaat caacttattg gtaaactcaa agtcgagatg aaactcgata
    17401 cgacacgcct cctggctcag cggcgtaaac ttccatccac caatcaattt cttgaacggc
    17461 ccatccacca gattcataag aatactttgg ttactggtca actggttgcg ggtagtaaac
    17521 gttttgctga tcccagcctt agagacatct accgccgcag tcatctgccc aggagtggac
    17581 tccagaatcc gacttccggt acaacctggc aaaaactgag gataagactg aacgtcattc
    17641 actaactgat acatttgctc cgcgctgtag ggtaccagtg cggtccggct aatctgaggc
    17701 ataacaattt ccatcaacaa aaatccaaca aataatatca tttatcctgc tgtaaaaaaa
    17761 acgctatccc ggcgctgggt aacatcgggt tcatgctaag atagagcctt gtcccccgca
    17821 ggattgatat ggggtatttt cgatttcaga ttaccgatga ttcacgacgc ttatgacgaa
    17881 gaaaaaagca cataaacctg gttcagcgac catcgcgctt aacaagcgcg cccgtcacga
    17941 atactttatc gaagaagagt tcgaagcggg acttgccctg caaggctggg aagttaaatc
    18001 cctgcgcgca ggaaaagcca atatcagcga cagctacgtc cttctgcgtg acggagaggc
    18061 atttctgttt ggcgctaaca tcacgccaat ggccgtggcc tccacgcatg tggtgtgcga
    18121 tcctacccgt acccgcaagt tacttctcaa ccagcgcgaa ctggactcat tgtacggtcg
    18181 cgtcaatcga gaaggctata ccgtagtggc gctctccctg tactggaaaa atgcctggtg
    18241 caaagtgaaa atcggcgtcg ccaaaggtaa aaaacagcac gataaacgtt cagatatcaa
    18301 agaacgcgaa tggcaggtgg ataaagcacg catcatgaaa aacgcccacc gttaaacctg
    18361 cactccaatt attgaccagt tcctcaccgc gcctccctct ccggcggcgc gaatgaacat
    18421 cttattggct atcacatccg acacaaatgt tgccatccca ttgcttaatc gaataaaaat
    18481 caggctacat gggtgctaaa tctttaacga taacgccatt gaggctggtc atggcgctca
    18541 taaatctggt atacttacct ttacacattg gggctgattc tggattcgac gggatttgcg
    18601 aaacccaagg tgcatgccga ggggcggttg gcctcgtaaa aagccgcaaa aaatagtcgc
    18661 aaacgacgaa aactacgctt tagcagctta ataacctgct tagagccctc tctccctagc
    18721 ctccgctctt aggacgggga tcaagagagg tcaaacccaa aagagatcgc gtggaagccc
    18781 tgcctggggt tgaagcgtta aaacttaatc aggctagttt gttagtggcg tgtccgtccg
    18841 cagctggcaa gcgaatgtaa agactgacta agcatgtagt accgaggatg taggaatttc
    18901 ggacgcgggt tcaactcccg ccagctccac caaaattctc catcggtgat taccagagtc
    18961 atccgatgaa gtcctaagag cccgcacggc gcaagccctg cgggcttttt tgtgctccga
    19021 atttgtcccg cgaagtctga tgccaactaa ttaaatccga tccttttagg caccttgtta
    19081 ggcacttcat aaagctatat tgtctttgag gtgcctaaaa ctatggaaac ccggcaatgg
    19141 caagacaaac caaacctcta tccgttaagg aaatcgaatc tgctaaaccc aaggaagcgg
    19201 actacgttct ctatgatggc gatggccttg agctactcat taaatccagc gggagtaaaa
    19261 tctggcagtt tcgctacatt cgccctgtca ccaagacacg agcaaagaaa agcataggcc
    19321 cttacccgtc agttaccctt gccgatgcca gaaactaccg tgcagagtcc cgctcactcc
    19381 tagctaaaca gattgatccg caggaacatc agcaagaaca attgcgtagt tcgctggaag
    19441 ccaaaaccaa tactttccag ctcgtagctg aacgttggtg gaatgtgaag aaagccagtg
    19501 tgacagagga ctatgcggaa gatatctggc gctctcttga aagagatgtc tttcctgcga
    19561 ttggcgacgt tagcgttaca gatattaaag ctcatacact ggttcaggcc gtacaaccgg
    19621 ttcaggccag aggagcactg gaaacggttc gtcgcctgtg tcaacgtatt aatgaggtca
    19681 tgatctatgc ccagaacaca gggctgattg atgctgttcc cagcgttaat atcggtaaag
    19741 ccttcgaaaa gcctcagaaa aagaacatgc ccagcattcg accggatcag ctatctcaac
    19801 tgatgcagac aatgcgaaca gccagcatta gcctttccac acgctgcctg ttcatgtggc
    19861 aacttcttac tattacccgc cctgccgaag cggctgaagc tcgctgggaa gaagtagaca
    19921 tagaagcgca agagtggaag attcccgcag cacgcatgaa aatgaaccgc gaccatactg
    19981 ttccattgtc agatgaagca atagctgtgc tggagatgat gaaaccgcta agcggcaacc
    20041 gagaatttat ctttcctagc cgtatcaagc ccaaccagct tatgaacagt cagactgtta
    20101 acgcctcact aaaacgcgca ggttttggtg gtgtgcttgt ttcgcatggt ctgagatcaa
    20161 ttgccagtac ggctcttaat gaacaaggct ttccgcctga tgccattgag gcagcattgg
    20221 cccatgtgga taagaatgag gttcgtcgtg cttataaccg cagcgattac ctggaacagc
    20281 gtcgcccgat gatgcaatgg tgggcaaatt ttgtgatggc agcggatcgt ggaagcatga
    20341 ttgaaggtgg gataaaagga atgaagctag ttgggtgact aaatagctgc gcggcatagt
    20401 gtcccacttt cagccagccc ttgcaggtta agccaccaga cagtaaacgg cgggtgtcgc
    20461 gttattaggc gcagctattt agtagtaata ttaggattgg tgattctgca ctccagaagt
    20521 t
//
LOCUS       DAEBXN010000076        20509 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.076, whole genome shotgun sequence.
ACCESSION   DAEBXN010000076 DAEBXN010000000
VERSION     DAEBXN010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20509
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.076"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..306
                     /locus_tag="KJL99_002767"
     CDS             <1..306
                     /locus_tag="KJL99_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HBD4437438.1"
                     /translation="LLIARWELTAEQAVAQQLKNQVSKGNLIDTGFCIFALSKLAMAL
                     SSTLDSIPLSMQRQFPDLTPRHIDHLKILIAKGANQCARAGDKLPDLLDEYIRTTTE"
     gene            278..2188
                     /locus_tag="KJL99_002768"
     CDS             278..2188
                     /locus_tag="KJL99_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HBD4437439.1"
                     /translation="MNISEQQLSNMMVAVTIALQPLVRVLPVTAVEWADQNYYLPKES
                     SYGEGEWKTLQFQVAIMNCMGNDEIRTVNLIKSARVGYTKMVLGVIGYFIEHKSRNTL
                     LFQPTDSAAEDFMKSHVEATIRDVPCLKKLSPWLGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GSCQIEKAANESAHFMRFYVPCPHCGEEQYLKFGDDATSFGLKWEKGKPETVYYLCEH
                     NGCVIRQSELDQTGGRWICDNTGMWTCDGLTFFSASGNEIPPPRSITFHIWTAYSPFT
                     TRVQIVCDWLDALKDPNGVKTFVNTTLGETWEEAVGEKLDHQVLMDKVVPYTATVPVR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYR
                     HADGTEMTISRVCWDIGGIDGEIVYQRSKKHGIFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKADPTPVNEATSYAIRFPDNPEIFSQTEAQQLVAEEL
                     VEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSVQRWQLDLAVQLHPARMQLHNR
                     PLKNWQRSCPEE"
     gene            2189..2392
                     /locus_tag="KJL99_002769"
     CDS             2189..2392
                     /locus_tag="KJL99_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBD4437440.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLNELN
                     RAINDAESVLGTTRRRRPLGVRL"
     gene            2389..3982
                     /locus_tag="KJL99_002770"
                     /pseudo
     CDS             2389..3982
                     /locus_tag="KJL99_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            3972..5477
                     /locus_tag="KJL99_002771"
     CDS             3972..5477
                     /locus_tag="KJL99_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HBD4437441.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADTQVRGVLLDIDSSGGQATGAFDCADMIYRLRQLKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMGHVSYAGHLAQAGVDITLIYAGSHKVDGN
                     QFESLPAKVRQDMQQRIDAARRMFAEKVAMYTGLSVDAVMGTEAAVFEGQSGIEAELA
                     DELINASDAISVMAATLNTHDTGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAVAAPQQSGVSAQSEADRIMACEEAKGREQLAATLAAMPE
                     MTVEKARPILAASPQANAGPSLRDQIMALDEAKGAEAQAEKLAACPGMTVENARAVLA
                     AASGRAEPVSASTTAMFEHFMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
     gene            5514..5861
                     /locus_tag="KJL99_002772"
     CDS             5514..5861
                     /locus_tag="KJL99_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HBD4437442.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGCSGISSATPALTPLMLD
                     EDTGKLVVWDGQKAGHVVGILVLPLEGTETVLTYYKSGTFATEAIHWPERVDTHKKAN
                     AFAGSALSHAALP"
     gene            5919..6947
                     /locus_tag="KJL99_002773"
     CDS             5919..6947
                     /locus_tag="KJL99_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HBD4437443.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     GPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGEQFETVEVDFGRSVGN
                     NITQAGGTEWSAQDRDTFDPTHDLDAYCDFASGTINIAIMDGKVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPDGTLVLG
                     NTAAEGIRCYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            6999..7385
                     /locus_tag="KJL99_002774"
     CDS             6999..7385
                     /locus_tag="KJL99_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HBD4437444.1"
                     /translation="MATKEENLKRLCELAERLGREPDVSGSAADIAQRVAELEEELGD
                     AGEVAEPDTYSPSKDDPTAHKGESVPEQPGIVTGDETGQVTVVALATLHTKCNGSVIF
                     VSPGTSFRVSAEVAASMAAGGLAKRQ"
     gene            7397..7774
                     /locus_tag="KJL99_002775"
     CDS             7397..7774
                     /locus_tag="KJL99_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBD4437445.1"
                     /translation="MDGFDNLFDAALAGVNEVILRDMGISAVITSGELEGTHLTGVFD
                     DPESISFVAGGIRLEDSSPCLFVKTADISQLRRQDTLTIGDDSFFVDRITPDDGGCCY
                     IRLRRGSPAPQNNARMRRYDEGT"
     gene            7761..8345
                     /locus_tag="KJL99_002776"
     CDS             7761..8345
                     /locus_tag="KJL99_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBD4437446.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQTSAWAVNRVAAKIVSVATRQVAQ
                     NTVAGDNQVKGIPLKTVRERVRLLKASPSGKMYARMRVNRGNLPAIKLGTAQVRLARS
                     RHGSNSRHRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKIPMSGPL
                     TQAFEDARDRIIAAEMPKQLGYALKQQLRLHLSK"
     gene            8342..8743
                     /locus_tag="KJL99_002777"
     CDS             8342..8743
                     /locus_tag="KJL99_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBD4437447.1"
                     /translation="MNRHTQIRQSVLARLREQCGDSATFFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFVRAQAPDSELDTWMENTIFPAMKDIPVLSGLID
                     TMIPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            8754..9494
                     /locus_tag="KJL99_002778"
     CDS             8754..9494
                     /locus_tag="KJL99_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HBD4437448.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVTLSGLVATVKG
                     VKQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            9553..9939
                     /locus_tag="KJL99_002779"
     CDS             9553..9939
                     /locus_tag="KJL99_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HBD4437449.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRTAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASIMTTWSYDALGAGAER
                     VLSLSGMGTIENAGDDDHEALTPEKS"
     gene            9948..10277
                     /locus_tag="KJL99_002780"
     CDS             9948..10277
                     /locus_tag="KJL99_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HBD4437450.1"
                     /translation="MQFVMRLAREFRRADWRRMLSDMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            10249..13290
                     /locus_tag="KJL99_002781"
     CDS             10249..13290
                     /locus_tag="KJL99_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBD4437451.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTMQAASSAATAASAHAQTVEKNARAHERMAREVEQTRLRVDALNQK
                     MREEQAQARALAEAQDKAAAAFYRQIDSVKQAGAGLQELQRIQQQIRQARNSGGVGQQ
                     DYLALISEITAKTRALTQAEEQATRQKAAFIRQLKEQATRQNLSSSELLRARAAQLGV
                     SSAAEVYIRKMERAGKATHSLGLKSAAARRELGVLISQMARGNFGALRGSGITLANRA
                     GWTGALMSPKGMMTGGVIGGLVAAVLGLGKAWHDGRKEGEKFNRQLALTGHYAGVTVG
                     QLWKLSRAISGNGITQHAAAGALAQVVGSGAFHGNDIGMVAKAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNALHFLNATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     KRTSDIDNNLNALGSTLQTLSDWWKQFWDAAMNIGREDSLDAQIDALQEKIQRAKKYP
                     WTNASTQVEYDQQRLNDLQEKKRRKDLQDAKAQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKAIKKNTRATRNDEATRLLLQYS
                     QQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLARKN
                     ELIQALTLLDVKQQELQKQTALNDLRKKTVQLTSQLADKERALREQHNLDIATAGMGD
                     KQRQRYQAQLRIRQEYRQQLQQLENDSRQKGTYGTEDYRRAEEVLKGSLKRQLNENKR
                     YWQELEVAQGDWKNGAMRAFQNFTADADNAAGTAEQMFTVAFSSAGNALATFCTTGKL
                     NFKSFTSSLLSDMARIMAQMAMMQAVKGVGSLFGFTTNADGGVYQSADLSRYSGTVVN
                     RPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADTGGSGMVMFAPQYNIEINND
                     GTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            13290..13619
                     /locus_tag="KJL99_002782"
     CDS             13290..13619
                     /locus_tag="KJL99_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBD4437452.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKGEHPSLKAFLERHGGVRAFQWTPPYDWKLIRVVCRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            13619..14317
                     /locus_tag="KJL99_002783"
     CDS             13619..14317
                     /locus_tag="KJL99_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HBD4437453.1"
                     /translation="MQDIRQETLNECTRAEQSARVELWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGSGFELNGRGCAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            14322..15065
                     /locus_tag="KJL99_002784"
     CDS             14322..15065
                     /locus_tag="KJL99_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBD4437454.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            15002..15604
                     /locus_tag="KJL99_002785"
     CDS             15002..15604
                     /locus_tag="KJL99_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HBD4437455.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTCFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            15665..19144
                     /locus_tag="KJL99_002786"
     CDS             15665..19144
                     /locus_tag="KJL99_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HBD4437456.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVDGPVDGLKS
                     VLLNGTPVLDSEGKTNFSGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNVVEVNWIDPDNGHETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGNGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDRRGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFRQ
                     LALGNYSLTVRAVNARGQQGDPASVSFRIAAPAAPVTIELIPGYFQITVVPKLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAVGRASDDAEGYLSFYKGLINKTHLGKELWTQIDNGQLAPDLTEIRTSITNVSN
                     EITQTVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIEN
                     TPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     PAFSLTPGGRLTAKNADISGNVNANSGTLNNVTINKNCRVLGKLSANQIEGDLVKTVG
                     KPFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFRGAKHERKNNNIYSSCRLI
                     VKKNGAEIYNRTTLDNTLIYTGVIDMPAGHGHMTLEFSVSAWLVNGWYPTASISDLLV
                     VVMKKSTAGISIS"
     gene            19212..19811
                     /locus_tag="KJL99_002787"
     CDS             19212..19811
                     /locus_tag="KJL99_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HBD4437457.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHRSTLSAGYLHASTNAPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     LSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDNRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            19875..>20509
                     /locus_tag="KJL99_002788"
     CDS             19875..>20509
                     /locus_tag="KJL99_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HBD4437458.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     MKRFEEMVAQAQQSAGAAAGNAQQTAQDVAAAATARDDAQRFAEKARQDATVTAEDRK
                     ATAEDVTSTGANAAAAGQSAQDAAGYARAAEQAKNDIDAALTGTLKMANHLS"
BASE COUNT         4796 a         4853 c         6474 g         4386 t
ORIGIN      
        1 ctactcattg ccagatggga actgaccgca gaacaggctg tcgcacagca gttaaaaaat
       61 caggtgtcaa aaggaaatct tatcgatacc ggattttgta tttttgccct tagtaagctg
      121 gcgatggctt tatccagtac gcttgactcc attcctttgt ccatgcaacg acagttcccg
      181 gatttaacgc cgcgtcatat tgatcatctg aaaatcctta ttgctaaggg agcgaatcag
      241 tgcgcgcgtg cgggggataa attaccggat ttactcgatg aatatatcag aacaacaact
      301 gagtaatatg atggtggcag tcactatcgc actgcagcca ctggtcaggg tattgccggt
      361 gacggctgta gaatgggccg atcaaaatta ttacctgcct aaagaatcgt catacggtga
      421 gggggaatgg aaaacgctgc agtttcaggt cgccatcatg aactgcatgg gcaatgacga
      481 gattcgtaca gttaacctga ttaaatctgc ccgtgttggt tatacaaaaa tggtgcttgg
      541 tgtgatcggg tattttattg agcacaaatc ccgcaacact cttctttttc agccgacgga
      601 ctcagctgct gaagatttta tgaagtctca cgtggaagca acaattcgtg atgttccttg
      661 cctgaaaaaa ctttctccct ggctgggccg gaaacaccgg gataatacgc tcacactgaa
      721 gcgtttttcc tctggtgtgg ggttctggtg tttgggtggt gctgctgcca aaaactaccg
      781 tgaaaaatct gtcgatgtgg tctgctatga cgaactctcc tcgtttgaac cggatgtgga
      841 aaaagaaggt tcgccaacac tgcttggcga taaacgtatt gaaggttcgg tatggccaaa
      901 atccatacgc ggttcaacgc caaaaattaa aggctcctgt cagattgaaa aagctgcgaa
      961 tgaatctgcg catttcatgc ggttttatgt cccttgccct cattgcgggg aggagcagta
     1021 tctgaagttt ggcgatgatg cgacgtcgtt tggcctgaaa tgggagaagg gtaaaccgga
     1081 aacggtgtat tacctgtgtg aacataatgg ctgcgtgatc cgtcagtctg aacttgatca
     1141 gaccgggggg cgctggattt gtgacaatac cgggatgtgg acgtgtgacg gtctgacgtt
     1201 tttcagtgct tcgggtaatg aaattccgcc gccgcgctcc atcactttcc atatatggac
     1261 ggcgtacagt ccgttcacca ccagggtaca gatagtctgt gactggctgg atgcactgaa
     1321 agatcccaac ggcgtgaaaa cttttgtgaa caccacgctg ggcgagacct gggaagaggc
     1381 cgtgggcgaa aaactcgatc accaggtact gatggataag gttgtgcctt acacggcaac
     1441 ggtgcctgtc cgggtggttt atctgacggc gggcattgac tcgcagcgaa accgttttga
     1501 gatgtatgtc tgggggtggg ctccgggaga ggaagccttt ctggtggata aaatcatcat
     1561 catgggacgt cctgatgagg aagagacgct gttacgtgtg gatgcggcga tcaataaaaa
     1621 ataccgccat gcagacggta ccgaaatgac catttcccgt gtctgctggg atatcggggg
     1681 gatcgatggc gaaatcgtgt atcagaggtc aaaaaaacac ggtattttcc gggtactgcc
     1741 ggtaaaaggt gcctctgtct atggcaagcc ggtgatcacc atgccgaaaa cccgcaatca
     1801 gcggggtgtg tatctgtgtg aggtggggac ggataccgca aaagaaattc tctatgcccg
     1861 aatgaaggcc gatcccaccc ctgtgaatga ggccacatcg tatgccatcc gctttcctga
     1921 taatccggag attttttcgc agacagaggc acaacaactg gtggcggagg aactggtgga
     1981 aaagtgggaa aaaggaaaga tgcgtctgtt gtgggataac aaaaagcggc gtaacgaagc
     2041 gctggactgc ctggtgtatg cctatgcggc attacgtgtg tccgtgcaac gctggcaact
     2101 tgatctggct gtgcagttgc atcccgcaag gatgcaactg cacaaccgac ccttgaagaa
     2161 ctggcagcga agctgtccgg aggagtgaat ggttacagtc gctgaactgc aggcgctgcg
     2221 tcaggcgcgc cttgatttat taaccggtaa acgggttgtg tcggtacaga aagacggtcg
     2281 caggatcgaa tatacggcag cgtccctgaa tgagcttaac cgtgcgatca acgatgcgga
     2341 gtcggttctg ggtacgaccc gacgccgccg accgctggga gtgaggttat gaaacgaacg
     2401 cctgtcctga ttgatgtgaa cggcgttccg cttcgtgaga gtctcagcta caacggaggc
     2461 ggcgcaggat ttggcgggca aatggcagag tggttaccac cggcgcagag tgccgatgcg
     2521 gccctgttgc ctgcattgcg tctggggaat gcccgggcag atgatctggt gcgcaataac
     2581 ggtatcgcgg ctaatgcggt ggccctgcat aaggatcata tcgtcgggca catgtttctt
     2641 atcagctacc gtctgaactg gcgttggctg gggatgcggg agaccacggc aaaaagtttt
     2701 gtcggggagg tggaagcagc ctggtcggaa tacgcagaag gaatgtttgg cgagatcgac
     2761 gtggaaggga aacgcacgtt tacggaattt atccgtgaag gggtgggcgt gcatgccttt
     2821 aacggcgaaa tctttgtgca gccggtctgg gatacggaaa ccacgcagtt attccgtacg
     2881 cgttttaaag ccgtgagtcc gaaacgggtg gacacgccag gacacggtat ggggaaccac
     2941 tttctgcggg ccggggttga ggtcgatcgg tatgggcggg cgattgcgta ccatatctgt
     3001 gaggatgatt ttccgttctc tggtgccgga cgctgggagc ggatcccgcg tgagcttccc
     3061 accgggcgtc cggccatgct gcatattttt gagccggtgg aggacgggca gacccgtggg
     3121 gctaatcagt tttacagcgt aatggagcgg atgaagatgc tggactccct gcaggccacg
     3181 cagcttcagt cggccattgt gaaggcgatg tatgcagcca caatcgaaag tgaacttgat
     3241 accgaaaagg catttgaata tatcgcgggg gcaccgcaag gtcagcagga taatccgctt
     3301 attaatattc tggagaagtt cacccgctgg tatgacacga ataacgtgac gctgggcggt
     3361 gttaaaattc cgcacctttt ttcctggcga tgcgctgaac ctgcagacag cgcaggattc
     3421 agacaatgga ttttcggcgc ttgaacaggc gctgctgcgg tatattgcag ccggtcttgg
     3481 cgtttcctac gaacagttgt cccgtgatta ctcgaaggtc agttactcaa gtgcccgcgc
     3541 ctccgccaat gagtcgtggc gctattttat ggggcggcga aaatttattg cggcccggct
     3601 ggccacgcag atgttttcct gctggctgga agaggcgctt cttcggggga ttattcgtcc
     3661 gccacgggcg cgttttgatt tttaccaggc gcgttcagcc tggtcacggg cagagtggat
     3721 tagctccgga agaatggcca ttgacgggct caaggaggtc caggaatcgg tgatgcgcat
     3781 tgaggccgga ctgagcacgt atgagaaaga actggcactg atgggcgaag attatcagga
     3841 cattttccgc cagcaggtca gggaatccgc agagcgcgaa aaagccggac tctcacgtcc
     3901 ggtgtggata gcccaggcgt atcagcagca gatagcggag agtcgcaggc cggaagagga
     3961 gacaacatca cgtgagacgt aatctttcac atattatcgc tgcggcattc aatgagccgc
     4021 tgcttctgga gcccgcctat gcgcgggttt tcttttgcgc gctcgggcgt gagatgggcg
     4081 cagcaagcct ttcggtaccg caacagcagg tacagcttga cgctcccggg atgctggctg
     4141 aaacggacga gtacatggcc ggaggcaaac gacctgcccg tgtttaccgg gtggtgaacg
     4201 gtattgctgt actgccggtg accggcacgc tggtacaccg gctgggtggc atgcggccat
     4261 tttccggaat gactggctat gacggcattg tcgcctgtct tcagcaggca atggcggaca
     4321 ctcaggtacg gggcgtactg ctggacattg acagttctgg cgggcaggcc accggcgcgt
     4381 ttgactgcgc tgacatgatt taccgtctcc ggcagctgaa gcctgtctgg gcactgtgca
     4441 atgacacggc ctgttcagcc gccatgttac tggcatcggc ctgctcccga cggctggtta
     4501 cccagacatc ccgtatcggc tccattgggg tgatgatggg ccatgtcagc tatgccggtc
     4561 atctggcaca ggccggtgtg gatatcacgc tgatttacgc cggatcgcac aaggtggatg
     4621 gcaatcagtt tgaatccctg ccagcaaagg ttcgccagga catgcagcag cgcattgatg
     4681 cggcgcgccg gatgtttgct gaaaaagtgg cgatgtatac cggtttatct gtggatgcgg
     4741 ttatgggaac agaggccgcc gtttttgaag gtcagtccgg tattgaggcc gaactggcgg
     4801 atgaattaat caatgcgtcg gacgccatca gcgtgatggc tgcgacgctg aacacacacg
     4861 atacaggagg cactatgccg caattaactg caacggaggc tgccgcgcag gagaaccagc
     4921 gagtgatggg gatcctgacg tgtcaggaag cgaaaggacg tgaacaactg gccacgatgc
     4981 tggcagggca acagggtatg agcgttgagc aggcccgggc gattctggcc gtggcggcac
     5041 cgcaacaatc tggtgtatct gcgcagagtg aagccgatcg cattatggca tgtgaagagg
     5101 cgaaaggacg tgaacaactg gcggcaacgc tggcggcgat gccggagatg acggtggaaa
     5161 aagcccgtcc gattctggct gcttcaccgc aggcgaatgc cggaccctca cttcgtgatc
     5221 agattatggc cctggatgag gcaaaagggg cagaggcgca ggctgaaaaa ctggcggcct
     5281 gtccggggat gaccgtggag aatgcccggg ctgtactggc ggcagcctca ggcagggcag
     5341 aaccggtttc tgcatccaca accgccatgt ttgaacattt catggcgaac cattcaccgg
     5401 cagcggtaca gggtggcgtg ccacagacgt cagcagacgg tgatgcggac gtgaaaatgc
     5461 tcatggccat gccatgaagt cagtgctgac catcaatatg aggtttttac aatatggtaa
     5521 cgaaaaccat cactgaacag cgtgcggaag tacgtatttt tgccggtaat gatccggccc
     5581 ataccgccac aggctgcagc gggatttcct cggcaacacc ggctctgacg cccctgatgc
     5641 tggatgagga taccgggaaa ctggtggtct gggacggaca gaaggcaggc catgtggttg
     5701 gcatactggt actgccgctt gaaggtacag agacggtgct gacgtattac aagtcgggga
     5761 cctttgcgac ggaggccatc cactggcctg agagagtgga tacacacaaa aaggcgaatg
     5821 cctttgccgg cagtgccctg agtcacgcag cgctgccgta acattttatc aggtcactgc
     5881 ggtagcctga ctgatttctg aatgaaagga actgatttat gggattgttt acgacccgcc
     5941 agttactcgg ttataccgaa caaaaagtta aatttcgtgc gctgtttctg gagctgtttt
     6001 tccgccgtac ggtgaatttc cataccgaag aggtaatgct ggacaaaatt accggaaaaa
     6061 cgccggtggc ggcctatgtc tccccggttg ttgaaggaaa ggtgttgcgt caccgtggcg
     6121 gtgaaactcg tgtactgcgc ccgggctacg ttaagccgaa acacgaattt aattaccagc
     6181 aggcggtgga gcgtcttccc ggtgaagatc cggcccagct gaacggcccg gcttaccgtc
     6241 gcctgcgtat catcaccgat aacctcaaac aggaagagca cgccattgtc caggtggaag
     6301 agatgcaggc ggtgaatgcc gtgctgtatg gcaaatacac catggaagga gagcagtttg
     6361 aaactgttga ggtcgatttt ggccggtcgg tgggaaataa cattacgcag gcaggaggga
     6421 cggagtggag cgctcaggac cgcgatacct ttgatccgac gcatgatctg gatgcgtact
     6481 gtgattttgc ttccggcacc atcaatattg ccattatgga tggtaaggtc tggcgtctgc
     6541 tgaatggctt taagctgttc cgcgaaaaac tggatacccg tcgtggctca aattcacagc
     6601 tggaaacggc agtgaaagac ctgggggcgg tggtgtcctt caaagggtat tatggtgatc
     6661 tggcaatcgt ggtggcaaaa acgtcctatg tggcagagga cggtaccgaa aaacgttatc
     6721 tgccggacgg cacactggta ctgggaaata cggccgcaga gggtattcgt tgctacggtg
     6781 ccattcagga tgcgcaggca ttgtctgaag gcgtggtggc gtcttcccgt tatccgaaac
     6841 actggctgac cgtgggtgat ccggcgcgtg aattcaccat gacgcagtcc gcgccgctga
     6901 tggtgctgcc ggatccggat gagtttgtgg tggtgcaggt gaaataatcc gtgagcggga
     6961 gctaaatgct cccgtttttc attcaatcag gagcgggtat ggctacgaaa gaagaaaacc
     7021 tgaaacgtct ttgtgagctg gctgagaggc tggggcgtga accggatgtc tccggtagtg
     7081 cggcagatat tgcgcagcga gtggcggaac tggaggaaga gcttggagat gcgggggagg
     7141 tcgcagagcc ggatacgtac tcaccatcga aagatgatcc gaccgcgcat aaaggcgagt
     7201 cagttcctga acagccaggc attgttaccg gagacgaaac ggggcaggtt acggtggtgg
     7261 cactggcgac actgcataca aaatgtaatg gttcagtgat atttgtttca ccgggaacgt
     7321 cgtttcgtgt ttctgctgag gtggctgcca gcatggcggc aggtggtctg gcaaaaaggc
     7381 agtaacagga gatgtcgtgg acggtttcga taatctgttt gatgctgcgc ttgccggtgt
     7441 caatgaagtc attctccgtg atatggggat cagcgccgtc attacgtccg gagaacttga
     7501 ggggacacac ctgacagggg tttttgatga tcccgaaagt atttcttttg ttgccggggg
     7561 gatccggctt gaggattcct caccgtgcct gtttgtgaaa acggcagata tatcgcagtt
     7621 acgccgtcag gatacgttaa ctatcgggga tgattctttc tttgtggatc gtattactcc
     7681 ggatgacggg ggatgctgtt atatccggtt aaggcggggc agtcctgccc cacagaataa
     7741 tgccagaatg aggcgatatg atgaagggac ttgagaatgc catccgcaat ctgaacagcc
     7801 tcgatacccg catggtgcca cagaccagcg catgggcggt aaaccgtgtg gcggcaaaga
     7861 ttgtttctgt cgccacacgg caggttgcgc agaataccgt tgccggggat aaccaggtga
     7921 aaggtattcc cctgaaaacg gtcagggagc gtgtcaggct gcttaaagcc agcccctcag
     7981 gaaaaatgta tgcccgcatg cgtgttaacc ggggtaacct gcccgccatt aagctgggta
     8041 ccgcacaggt cagactggcc cgctcccggc atggcagcaa ctcacgtcat cgtggcagcg
     8101 tactgaaagt ggggaaatac cttttccggg atgcgtttat tcagcaactg gcgaatggtc
     8161 gctggcatgt gatgcggcgt attgacggca aaaatcgtta ccccattgat gtggtgaaaa
     8221 tcccgatgtc cggaccgctg acacaggcat ttgaagatgc ccgcgaccgc atcattgctg
     8281 cggaaatgcc gaaacagctg gggtatgccc tgaaacaaca actgaggtta catctgagta
     8341 aatgaaccga cacacacaaa tccgtcagag cgtgctggca cgccttcggg aacagtgtgg
     8401 agacagcgcc acgttttttg acgggcttcc ggcatttatt gatgcgcagg aactgcccgc
     8461 cgtggcggtg tggctgagtg atgcgcagta caccggaaaa atgacggatg aggacgactg
     8521 gcaggctgtt ctgcatattg ccgttttcgt ccgtgcacag gcgccggatt ctgagctgga
     8581 tacgtggatg gagaacacca tatttccggc catgaaggat attccggtgc tttccggact
     8641 catcgacacc atgatcccac tcggttttaa ctatcaacgt gataatgaga tggccacctg
     8701 ggcgatggcg gaaatcacgt accagatcac gtacacgaat taaggaggtg gtaatgacca
     8761 caccaaatcc actggcaaag acgaaaggtg cggggacgac gttctggatg tataccggca
     8821 acggcgatgc gtttgcgaac cctttgtcgg acactgactg gctgcgtctt gcgatggtga
     8881 aggatctgca acctggcgaa atgaccgctg atgcagaaga tgacacttat ctcgatgatg
     8941 aagatgcaga ctggaaaacg acaacccagg ggcagaaatc cgttggtgat acttcggcga
     9001 cgctggcctg gcgtccgggt gacagcgggc agaaaaaact ggttcagttg ttcgactccg
     9061 gtgaagtctg cgcgtttcgt atcaaatatc ccaacggcac tgttgatgtt ttccgcggct
     9121 ggctgagctc actgggtaaa accattgcct caaaagacgt gatgacccgc actgtgaaaa
     9181 tcagcggtgt ggggcgtccg tatctggcag aggaaggcac tgaaaccgtg ggtgttaccg
     9241 gactgacggt ggcaccggca tctgtcagtg tcaaagcggg agcaaccacc acactgacct
     9301 ttacagtaaa acctgacggg gccagtgaca aagcgatcag tgtgcattcg tcagatcctc
     9361 agactgcttc ggtgaccctg agcgggcttg tggccacggt gaaaggcgtg aagcagggca
     9421 gtgtcagcat cgtgggcatg acctctgacg gagagtttgt ggcagtggct gcggtgaccg
     9481 tcagcgcacc ataacaggac gatactcatc attgccccgg ttatccgggg ctttttgcat
     9541 ccggagaaca tgatgtttct gaaacaggat acgtttaatt atgaaaaaca gtccgtggtg
     9601 ctcagtgagc tgtccgggct gcagagaatt gaatatctga cgtttgttca gcagcgaacg
     9661 gcaaagtttg atgcacagga gggagaactg ccggaggctg aacgacagat tgcttttctg
     9721 cgtatgggaa tggatatcaa tgcctggctg gtttcccgct cactgtggaa tgctgagcag
     9781 tctcaggatg ttgagacgct ttgcgcatcc attatgacaa catggtcgta tgatgcgctg
     9841 ggcgcggggg cggagagggt tctgtcgctg agcggtatgg ggaccattga gaatgccggg
     9901 gatgatgatc atgaggcgct gacgccggaa aagtcctgac gcgggaaatg cagtttgtca
     9961 tgcggcttgc ccgggagttc cggcgggcag actggcggcg gatgctgtcg gatatgtcgg
    10021 ccactgagct tggtgagtgg ggcgattatt tccggatgca gagcttcagt gatgtgtgga
    10081 tggatgcgca gtttgcctcg ctgaaggcat tgatcgtgag aatggtgtcc ggcagcagcg
    10141 atgctgcggt ggctgatttc agccttttac cggaagagaa cgggataccg gagcgaacgg
    10201 acgaagaact gatgcatctt ggggaaggta tttccggagg tgtgcgttat ggaccagata
    10261 gccaacctgg tcattgattt ggggattgat gcggcagagt ttaaaaatga aattccccgt
    10321 atcaaaaacc ttctgaatgg tgcagccagc gatgcagaac ggtcttctgc ccgtatgcag
    10381 cgttttatgg agcgtcagac tcaggcggcc cggcagacaa tgcaggcggc ttcttcggct
    10441 gcaacagcag catcagccca tgcgcagacg gtggagaaga atgcacgggc tcatgaacgc
    10501 atggcccgtg aggtggaaca aacccgtctg cgcgtggatg ccctgaatca gaaaatgcgc
    10561 gaggaacagg cgcaggccag ggcactggcg gaggcgcagg ataaagcggc tgccgccttt
    10621 tatcgccaga ttgacagtgt gaaacaggcc ggtgcggggc ttcaggaatt acagcgtatt
    10681 cagcagcaga tccgacaggc cagaaacagt ggcggggttg gtcagcagga ttatctggcg
    10741 ctgatttcgg agatcacggc gaaaacccgt gccctgacgc aggcagagga acaggccacc
    10801 cggcagaaag cggcgtttat ccgccagctt aaagagcagg caacccgcca gaatctgtcg
    10861 tcttctgagt tgcttcgcgc cagggcggct cagctggggg taagcagtgc tgcagaagtg
    10921 tatatccgca aaatggagcg ggcaggaaaa gccacacatt cgctggggct gaaaagtgcg
    10981 gcagcccggc gggagctggg ggtgttaatc agtcagatgg cgcgaggcaa ttttggtgcg
    11041 ctgaggggat ccgggataac gctggctaac cgtgccggat ggacaggcgc actgatgtcg
    11101 ccgaaaggca tgatgactgg cggcgttatt ggcggacttg tcgcggcggt cctgggtctg
    11161 ggtaaagcct ggcatgacgg ccggaaggag ggcgagaaat ttaaccgtca gctggcgctg
    11221 acgggacatt atgccggtgt cactgtcggg cagttgtgga aactcagccg ggccatatcc
    11281 gggaatggta tcacgcaaca tgcggcagcc ggtgcgctgg cacaggtggt gggcagtggg
    11341 gcatttcatg gaaacgatat tggtatggtg gcgaaagctg ccgcacagat ggagcgatcg
    11401 gttggccagt cggtcagcga taccataaat cagtttaagc ggctgaagga tgatcctgta
    11461 aatgccgcga aggcgctgga caatgcgctg cattttttga atgccactca gcttgagcag
    11521 atacgcgtcc ttggggagca ggggcggtcc agtgatgcgg cccggatcgc catgtctgcg
    11581 ctggcagagg aaaccggtaa acgcacatct gatattgata ataatctcaa tgcgctgggt
    11641 agtacgctgc aaaccttgtc tgactggtgg aagcagttct gggatgcggc catgaatatt
    11701 ggtcgtgaag actcgctgga tgcgcagatt gatgcgttac aggaaaaaat tcagcgcgcg
    11761 aaaaaatatc cgtggacaaa cgcctccaca caggtggagt acgatcagca gcgtcttaac
    11821 gatcttcagg agaaaaaacg ccggaaggat ttgcaggatg caaaagcgca ggcagaacgg
    11881 aattaccagg agcaacagaa acgccggaat gctgaaaatg ccgcgctgaa ccggatgaat
    11941 gaaacggaag ctgcacgaca tcagcgggaa attgcgcgta ttaatgccat gcagtacgcc
    12001 gaccaggctg tcagggatgc ggcgatacag cgtgaaaatg aacgttacga aaaagccatt
    12061 aagaaaaata cacgggcaac ccgtaatgat gaggccaccc ggttattgct gcagtacagt
    12121 cagcagcagg cacaggtgga agggcagatt gctgctgcca gacagtcagc aggcattgcc
    12181 actgaaagga tgactgaagc gcataaacag cttctggccc tgcagcagcg catcagcgac
    12241 ctggacggga aaaagctgac ggcagatgaa aagagtgtgc tggcccgtaa gaatgagctg
    12301 attcaggcgc tgacgctgct ggatgtgaaa cagcaggagc tgcagaaaca gacagcgctt
    12361 aacgacctga gaaaaaaaac ggttcagctg accagccagc tggcagacaa agaacgtgca
    12421 ctgcgtgagc agcacaatct ggatattgcc actgcaggta tgggggataa gcagcggcag
    12481 cgctaccagg cacagttgcg catccggcag gaataccggc aacagttgca acagcttgag
    12541 aatgacagtc gccagaaagg cacttacggg acggaggact accggagggc tgaggaggtg
    12601 ctgaagggga gcctgaagcg acaactgaat gaaaacaaac gctactggca ggaactggaa
    12661 gtggcgcagg gcgactggaa aaacggtgcc atgcgggcgt ttcagaattt tacggcggat
    12721 gcggataatg cggcgggaac ggcagaacag atgtttacag tggcattcag cagtgccggt
    12781 aatgcactgg cgacattctg taccaccggt aagctgaatt ttaaatcctt cacctcttcc
    12841 ctgttgtcag atatggccag aattatggca cagatggcca tgatgcaggc ggtaaagggc
    12901 gtcggttctt tattcggctt cacgactaat gctgatggcg gtgtttacca gtctgctgat
    12961 ttgagtcgct acagtggcac ggtggttaac cgtccgacgt tttttgcttt tgcaaaaggc
    13021 gcgggtgtga tgggggaagc gggacctgaa gccattctgc cactgcgtcg cggtgctgat
    13081 ggtaagctgg gggttgtggc ggatactggt ggttcaggca tggtgatgtt tgccccgcag
    13141 tacaacattg agatcaataa cgacggcacg aacgggcaga taggtccggc tgccctgaag
    13201 gtggtttatg acctcgggaa aaaagcggcc gcggacttta tgcaacagca ggcccgtgat
    13261 ggtggccggt taagtggagc ataccggtaa tggagacgtt tcactggaaa gtgcgcccgg
    13321 atatgaatgt ggtatcagag ccgaaagtgg tgacagtgaa gctgggcgat ggttatgaac
    13381 agcgtcgtgc ggcgggactg aataaccagt tgtcgactta cagcgtgacg atacgtgttc
    13441 gtaaaggtga acacccatct ttaaaagcct ttctggaacg gcacggtggc gtccgcgcat
    13501 ttcagtggac gccaccttat gactggaaac tgatcagggt ggtttgtcgt aaatggtcgg
    13561 caagcgtggg ggcgttatgg gtgactgtaa cggccgattt tgaacaggtg gtgaactgat
    13621 gcaggatatc cggcaggaaa cactgaatga atgcacccgt gcggagcagt cagcccgggt
    13681 ggagctctgg gaaatcgatc tgacagaggt cggtggtgag cgttattttt tctgtaatga
    13741 gcagaacgaa aaaggtgagc cggtcacctg gcaggggcgg cagtatcagg cataccccat
    13801 tcaggggtcg gggtttgaac tgaacggcag gggctgtgct gcccgtccga cactgacggt
    13861 ctctaacctg cacggcatgg tcaccgggat ggcggaagat ctgcagagtc tggtcggcgg
    13921 aacggtggtc cggcgtaagg tttacgcccg ttttctggat gcggtgaact tcgtcaacgg
    13981 aaacagcgac gccgatccgg aacaggaggt gatcagccgc tggcgcatcg agcagtgcag
    14041 cgaactgagc gcggtcagtg cctcttttgt gctggccaca ccaacggaga cggatggcgc
    14101 ggttttcccg gggcgtatca tgctggcgaa tacctgtatg tggacttacc gttctgatga
    14161 gtgtggttac acgggcaggg cagtggctga cgagttcgac aaaccaacaa cggatatccg
    14221 gaaggacaaa tgcagcaagt gtatgcgcgg gtgtgagttg cgcaacaata ccggtaattt
    14281 cggcggtttc ctttccatca ataaactttc tcagtaaatc catgacacag acagaatcag
    14341 cgattctggc gcacgcccgg cgatgtgcgc cagcggagtc gtgtggcttc gtggtgagaa
    14401 cgccggaggg agacaggtat cttcccagcg agaatatctc cggtgagccg gaggaacggt
    14461 tccggatggc tccggaggac tggctgcggg cacaaatgca gggtgagatt gtggcactgg
    14521 tccacagtca tcccggtggt ctgccctggc tgagtgaggc tgaccggcgg ctgcaggtgc
    14581 agagtgattt gccgtggtgg ctggtctgcc gggggacgat tcacaagttc cgctgtgtgc
    14641 cgcatctcac cgggcggcgc tttgagcacg gggtgacgga ctgttacacg ctgttccggg
    14701 atgcttatca tctggcgggg attgagatgc cggattttca tcgcggggat gactggtggc
    14761 gtcacggtca gaatctctat ctggataatc tggaggccac agggctgtat caggtgccgt
    14821 tgtcatcagc acaaccgggc gatgtgctgc tgtgctgttt tggttcatcg gtgccgaatc
    14881 atgccgccat ttactgcggc gatggtgagc tgctgcacca tattcctgaa caactgagta
    14941 aacgagagag gtataccgac aaatggcagc gacgcacaca ctccctctgg cgtcaccggg
    15001 aatggcacgc atctgccttt acggggattt gcaacgattt ggccgccgca tcgaccttcg
    15061 tgtgaaaacg ggggctgaag ccatccgcgc actggccaca cagctcccgg cgtttcgtca
    15121 gaaactgagt gacggctggt atcaggtgcg cattgccggg cgtgatgcag gtgaaaccga
    15181 attgtctgcc cgtcttaatg agccgctggc aaatggtgca gtgatccaca tagtaccgcg
    15241 tctggtggga gctaaaagtg gcggtgtgtt tcaggcggtg ctgggggcag ctgttatggc
    15301 ggttgctata tggatgccgg gggtaggaat tatggcgagt aatctgctgt tttctctcgg
    15361 tgccagtatg acgcttggcg gtgttgcaca gatgctggca ccgaaagcca gaactccccg
    15421 tacacagaca acggataacg gcaaacagaa cacctgtttc tcctcactgg ataacatggt
    15481 tgcccagggc aatgttctgc ctgttctgta cggtgaaatg cgcgtggggt cgcgggtggt
    15541 atctcaggag atcagcacgg cagacgaagg ggatggtggt caggttgtgg tgattggtcg
    15601 ctgatgcaaa atgtttcatg tgaaaccgcc tgcgggcggt tttgtcgttt atggagcgtg
    15661 aggaatgggt aaaggcagca gtaaggggca taccccgcgc gaagcgaagg acaacctgaa
    15721 atccacgcag ttgctgagtg tgattgatgc catcagcgaa gggccggttg acggtccggt
    15781 ggatggatta aaaagcgtgc tgctgaacgg tacgccggtc ctggatagcg aggggaagac
    15841 aaacttttcc ggtgttacgg tggtgttccg cgccggcgag caggagcaga caccgccgga
    15901 ggggtttgaa tcttccggct cagagactgt gctgggtacg gaagtgaaat acgacacgcc
    15961 gatcacccgg accatcacgt cggcaaacat tgatcgtctg cgcctgacct tcggtgtgca
    16021 ggcactggtg gaaaccacct caaaggggga ccggaatccg tcggaagtcc gcctgctggt
    16081 tcagatacag cgtaacggtg gctgggtgac ggaaaaagac atcaccatta agggcaaaac
    16141 cacttcgcag tatctggcct cggtggtggt gggtaacctg ccgccgcgcc cgtttaatat
    16201 ccggatgcgc aggatgacac cggacagcac cacagaccag ctgcagaaca aaacgctctg
    16261 gtcgtcgtac accgaaatca tcgatgtgaa acagtgctac ccgaacacgg cgctggtcgg
    16321 cgtgcaggtg gattcagagc agttcggtaa ccagcaggtg agtcgcaatt atcatcttcg
    16381 cgggcgcatt ctgcaggtgc cgtcgaacta taacccgcag acgcggcaat acagcggtat
    16441 ctgggacgga acgcttaagc cagcatacag caacaacatg gcctggtgtc tgtgggacat
    16501 gctcactcat ccgcgctacg gcatggggaa acgtcttggt gcggcagatg tggacaaatg
    16561 ggcgctgtat gtcatcggcc agtactgcga ccagtcagtg ccggacggat ttggcggcac
    16621 ggagccgcgc atcacctgta acgcttacct gaccacacag cgtaaggcgt gggatgttct
    16681 cagtgatttc tgctcggcga tgcgctgtat gccggtatgg aacgggcaga cgctgacgtt
    16741 cgtgcaggac cgaccgtcgg ataaagtgtg gacctataac cgcagtaatg tggtgatgcc
    16801 ggatgatggc gcgccgttcc gctacagctt cagcgccctg aaggaccgcc ataatgtcgt
    16861 tgaggtgaac tggattgatc cggacaacgg tcatgagacg gcgacagagc ttgtggagga
    16921 cacgcaggcc attgtccgtt acggtcgtaa cgtcacgaag atggatgcct ttggctgtac
    16981 cagccgggga caggcgcacc gcgccgggct gtggctgatt aaaacggaac tgctggaaac
    17041 gcagaccgtg gacttcagcg tcggcgcaga agggcttcgc catgtaccgg gtgatgtcat
    17101 tgaaatctgc gatgatgatt atgccggtat cagcaccggt ggtcgcgtac tggcggtgaa
    17161 cagccagacc cggacgctga cgctcgaccg tgaaatcacg ctgccgtcct ccggtaccac
    17221 gctgataagc ctggttgacg gaaatggcaa tccggtcagc gtggaggttc agtccgtcac
    17281 cgacggcgtg aaggtgaaag tgagccgggt tcctgacggc gttgcagaat acagcgtgtg
    17341 ggggctgaag ctgccgacgc tgcgccagcg cctgttccgc tgcgtgagta tccgtgagaa
    17401 cgacgacggc acgtatgcca tcaccgccgt gcagcatgtg ccggaaaaag aggccatcgt
    17461 ggataacggg gcacactttg acggtgaccg gcgcggcacg gtgaatggtg tcacgccgcc
    17521 agcggtgcag catctgaccg ccgaagtcac cgcagacagc ggggaatatc aggtgctggc
    17581 gcgatgggac acgccgaagg tggtgaaggg tgtgagcttc ctgcttcgcc tgaccgtggc
    17641 agcggacgac ggcagtgagc ggctggtcag cacagccagg acgacggaaa ccacataccg
    17701 cttcaggcag ctggcgctgg ggaattacag tctgacagtc cgggcggtaa atgcccgggg
    17761 gcagcagggc gatccggcgt cggtatcgtt ccggattgcg gcaccggcag cgcctgtcac
    17821 tattgaactg ataccggggt attttcagat aacggtggtc ccgaaactgg ctgtatatga
    17881 cccgacggtg cagtttgagt tctggttttc ggaaaagcgg attgcggata tcaggcaggt
    17941 tgaaaccagc gcccgctatc ttggcacggc gctgtactgg atagccgcca gtatcaatat
    18001 caggccgggc catgattatt atttttacgt tcgcagtgtg aacaccgttg gcaaatcggc
    18061 atttgtggag gccgtcggtc gggcgagcga tgatgcggaa ggttatctga gtttttataa
    18121 agggttgatc aataaaacgc atctcggcaa ggagttgtgg acgcagattg ataacggtca
    18181 gcttgcgccg gacctgactg aaatcaggac gtccattacg aatgtcagca atgaaatcac
    18241 gcaaaccgtc aataaaaaac tggaaaatca gagtgctgca atccagcaga tacagaaagt
    18301 tcaggttgat acaaataata acctgaacag catgtgggct gtgaagctgc aacagatgaa
    18361 ggacggacgc ctttatattg cgggtatcgg tgccggtatt gagaatacgc cagcaggaat
    18421 gcagagtcag gtgctgctgg cggcagacag gattgcgatg attaatcctg cgaatggcaa
    18481 cacaaagccg atgtttgttg gtcagggcga tcagatattc atgaacgacg tgttcctgaa
    18541 acgcctgacg gctccgacca ttaccagcgg cggtaatcct ccggcatttt ccctgacacc
    18601 tggcggacgg ctgacggcga aaaatgccga tatcagcggt aacgtgaacg cgaactccgg
    18661 gacgctcaac aacgtcacga ttaataagaa ctgtcgggtt ctgggaaaat tgtccgcgaa
    18721 ccagattgaa ggcgatctcg ttaaaacagt gggcaaacct ttcccacggg actcccgggc
    18781 accggagagg tggccatcag ggaccattac cgtcagggtt tatgacgatc agccgtttga
    18841 tcggcaaatt gttattcccg cggtggcgtt tcgcggtgct aaacatgagc ggaagaataa
    18901 caatatttat tcgtcatgcc gcctgatagt gaagaaaaac ggtgctgaaa tttataaccg
    18961 aacgaccctg gataatacgc tgatatatac gggtgttatt gatatgcctg ccggtcacgg
    19021 tcacatgacg ctggagtttt ctgtatcggc atggctggta aatggctggt atcccacagc
    19081 aagtatcagc gatttgctgg ttgttgtgat gaagaaatcc acagcaggta tcagtatcag
    19141 ctgaatttta taacccagat acgggcacca gaaatggtgc cttttttatt gcagaaaagc
    19201 gagaggtaat tatgcgtaaa ctttatgctg ccattttgtc cgcagccatc tgtctggctg
    19261 tatccggtgc gcctgcatgg gcgtctgaac atcggtccac gctgagcgcg gggtatcttc
    19321 atgcctcgac gaacgctccc ggcagtgatg atctgaacgg gattaacgtg aaataccgtt
    19381 atgagtttac ggacacgctg gggctggtga cgtcattcag ctatgccaac gctgaagatg
    19441 agcaaaaaac gcattacagt gatacccgct ggcatgagga ttccgtgcgt aaccgctggt
    19501 tcagcgtgat ggcggggctg tctgtgcgcg tgaatgaatg gttcagcgcg tatgcgatgg
    19561 cgggcgtggc ttacagccgt gtgtcgacct tctccgggga ttatctccgc gtaactgaca
    19621 acaaggggaa aacgcacgat gtgctgaccg ggagtgatga caaccgccac agcaacacgt
    19681 ctctggcgtg gggggctggc gtgcagttta acccgaccga atccgtggcc attgaccttg
    19741 cttatgaagg ttccggcagt ggcgactggc gcactgacgg tttcatcgtg ggtgtcggtt
    19801 ataagttctg attagccagg taacacagtg ttatgacagc ccgctggttc aggcgggctt
    19861 tttgtggggt gaatatggca gtaaagattt caggtgtact gaaagatggg gcgggtaaac
    19921 ctgtcgtaaa ttgtgcgatt gaactgcggg ccagaagaac cagtccgacc gttgtggcac
    19981 acgttgttgc cacttgcgtg acggacaata acggtgctta tgtgattgag gctgagccgg
    20041 ggtattacga ggttgcgctt cactgtaacg gctggcagcc aacccgtgtc ggggatattg
    20101 atgtggcacc gactgatgca ccggggacac tgaacgcgtt tctgaatgca ccaaaggatg
    20161 gtgatttacg tccggaggtg atgaagcgct ttgaggaaat ggtggcgcag gcgcagcaga
    20221 gtgccggggc tgcagccgga aacgcacagc agacggcgca ggatgtggcg gcagccgcaa
    20281 cggcccgtga tgatgcacaa cgttttgcgg agaaagcccg acaggatgca accgtcacag
    20341 ctgaggacag aaaggccact gcggaagatg tgacaagcac aggagcaaat gcagccgcag
    20401 ccggacagag cgcacaggat gccgcaggtt atgcccgcgc agcagaacag gccaaaaatg
    20461 acattgatgc tgcgctgacc ggcactctga aaatggctaa ccatctgtc
//
LOCUS       DAEBXN010000077        20244 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.077, whole genome shotgun sequence.
ACCESSION   DAEBXN010000077 DAEBXN010000000
VERSION     DAEBXN010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20244
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.077"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(435..641)
                     /locus_tag="KJL99_002789"
     CDS             complement(435..641)
                     /locus_tag="KJL99_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HBD4437459.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            954..1043
                     /gene="kdpF"
                     /locus_tag="KJL99_002790"
     CDS             954..1043
                     /gene="kdpF"
                     /locus_tag="KJL99_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HBD4437460.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            1043..2716
                     /gene="kdpA"
                     /locus_tag="KJL99_002791"
     CDS             1043..2716
                     /gene="kdpA"
                     /locus_tag="KJL99_002791"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HBD4437461.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGAQQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPSP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2739..4786
                     /gene="kdpB"
                     /locus_tag="KJL99_002792"
                     /pseudo
     CDS             2739..4786
                     /gene="kdpB"
                     /locus_tag="KJL99_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
     gene            4795..5367
                     /gene="kdpC"
                     /locus_tag="KJL99_002793"
     CDS             4795..5367
                     /gene="kdpC"
                     /locus_tag="KJL99_002793"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HBD4437462.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWYPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5360..8040
                     /gene="kdpD"
                     /locus_tag="KJL99_002794"
                     /pseudo
     CDS             5360..8040
                     /gene="kdpD"
                     /locus_tag="KJL99_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
     gene            8037..8722
                     /gene="kdpE"
                     /locus_tag="KJL99_002795"
                     /pseudo
     CDS             8037..8722
                     /gene="kdpE"
                     /locus_tag="KJL99_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
     gene            8997..9101
                     /locus_tag="KJL99_002796"
     CDS             8997..9101
                     /locus_tag="KJL99_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HBD4437463.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            9412..11609
                     /gene="speF"
                     /locus_tag="KJL99_002797"
                     /pseudo
     CDS             9412..11609
                     /gene="speF"
                     /locus_tag="KJL99_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
     gene            11606..12925
                     /gene="potE"
                     /locus_tag="KJL99_002798"
     CDS             11606..12925
                     /gene="potE"
                     /locus_tag="KJL99_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HBD4437464.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMAPAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFIANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGGIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(12981..14621)
                     /gene="pgm"
                     /locus_tag="KJL99_002799"
     CDS             complement(12981..14621)
                     /gene="pgm"
                     /locus_tag="KJL99_002799"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HBD4437465.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDKANALMADGLKGVKRISLDEAMASGYVKEQDLVQPFVEGLTDI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(14647..15192)
                     /gene="seqA"
                     /locus_tag="KJL99_002800"
     CDS             complement(14647..15192)
                     /gene="seqA"
                     /locus_tag="KJL99_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HBD4437466.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            15377..16141
                     /gene="ybfF"
                     /locus_tag="KJL99_002801"
     CDS             15377..16141
                     /gene="ybfF"
                     /locus_tag="KJL99_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBD4437467.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            16281..16574
                     /gene="ybfE"
                     /locus_tag="KJL99_002802"
     CDS             16281..16574
                     /gene="ybfE"
                     /locus_tag="KJL99_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HBD4437468.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            16714..17244
                     /gene="fldA"
                     /locus_tag="KJL99_002803"
     CDS             16714..17244
                     /gene="fldA"
                     /locus_tag="KJL99_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HBD4437469.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            17454..17540
                     /gene="uof"
                     /locus_tag="KJL99_002804"
     CDS             17454..17540
                     /gene="uof"
                     /locus_tag="KJL99_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HBD4437470.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            17533..17979
                     /gene="fur"
                     /locus_tag="KJL99_002805"
     CDS             17533..17979
                     /gene="fur"
                     /locus_tag="KJL99_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HBD4437471.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(18063..18389)
                     /locus_tag="KJL99_002806"
     CDS             complement(18063..18389)
                     /locus_tag="KJL99_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4437472.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(18426..19832)
                     /gene="chiP"
                     /locus_tag="KJL99_002807"
     CDS             complement(18426..19832)
                     /gene="chiP"
                     /locus_tag="KJL99_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HBD4437473.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWIKADSQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFSAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
BASE COUNT         4704 a         5050 c         5355 g         5135 t
ORIGIN      
        1 cataacatct tattggatta gatatgttaa caattaatta ttcttaatat atcaataaag
       61 tatatattac tctctttact atctgttttt attgcggtag cgatactaat aacaaaattc
      121 ggaatttatt tagatgctgg aattagaaaa agggatgacg gctttctcac cttaccctga
      181 agtttcacga aagtgtgtaa ggaagtatgc aatctaactg gagatttctg atgatctagt
      241 cgcttagagc gacaatgaag gtgaccgcat cctgggtagc cctatgaaag gatactattg
      301 aaacgtgatg ctaaaacagt gatgaaatga ataatacgat ggaaacgtct tccatgttaa
      361 ttatcatgga agacgttgat gttgaacacc taaaccagcc ccctgatacg aggctggctt
      421 aagcgcgcag gcaatcagta aaaatcaccg gttgcctttt cggcctgatc catccacacc
      481 ggtttatcgc tggttttcga ccagacgcgg tgcaggtagc tgtaaaagcg ggcgcggtct
      541 ttccagaaaa gcatgaaagg cagcgccaga acgcccgctg caaccgcata tgtacggcgt
      601 aaaaagataa gccatgcagg atattctctg tagagttcca tacttttctc ccccttttgt
      661 atctacccgg tgaatagcac cggaaaaatg aatttgttta tctgatgaaa atagtaccgc
      721 cttttgtgta attttactac tcatccgacc acttattttt gcttattgat ggtttattta
      781 cattcacact gtaattaaat tacacaaaag ttaaattaat actaaccatc agttaaatca
      841 tggcctttgc catttttata ctttttttac accccgtccg cagatttttg cgaaatcttt
      901 gcagccagaa ttctactctt ccggtatcac ttttaggcca ctggaggtgc actgtgagtg
      961 caggcgtgat agccggcgtg ttgctggtgt ttttattact gggctatttg gtttatgccc
     1021 tgatcaatgc ggaggcgttc tgatggctgc gcaagggttc ttactgattg ccacgttttt
     1081 actggtatta atggtgctgg cgcgtccttt aggcagcggg ctggcgcggc tgattaatga
     1141 cattcctctt ccaggtacag cgggcgttga acgtatactc tttcgtttac ctggcgtctc
     1201 tgaccatgag atgaactgga agcaatatct ttgtgccatt ctcggcctga acatgctggg
     1261 gctggcggtg ctgtttttta tgttgctcgg tcagcactat ctgccgctta atccacagca
     1321 gttgccgggg ctgtcgtggg atctggcgct gaataccgcc gtcagctttg tcacgaatac
     1381 caactggcaa tcttatagcg gtgaaaccac gttgagctat ttcagccaga tggcaggctt
     1441 aacggtacaa aactttcttt ctgccgccag cgggattgcg gtgatttttg ccttcatccg
     1501 tgcgtttact cgccagagca tgagcacact cgggaatgcc tgggtcgatc tgctacgcat
     1561 cacgttatgg gtgctagtcc ctgtggcgtt gttgatcgca ctgtttttta ttcaacaagg
     1621 tgcgcagcaa aactttctgc cctatcaggc agtgaatacc gttgaaggag cgcaacagct
     1681 gttacccatg ggaccagtag cttctcagga agcgatcaag atgctcggta ctaacggcgg
     1741 tggcttcttt aatgccaact cgtcgcatcc gtttgaaaac ccaaccgcac tgaccaactt
     1801 cgtgcagatg ctggcgatct tcttgatccc aacagcgctg tgctttgcct ttggtgaagt
     1861 gacgggcgat cgccgccagg ggcgcatgtt gctgtgggcg atgtcagtga tttttgtcat
     1921 ctgcgtaggc gtggttatgt gggcagaagt tcagggtaat cctcatctgc tggcgctcgg
     1981 cgcagacagc agcatcaata tggaaggtaa agagagccgt ttcggcgtgc tggtcagtag
     2041 cctgtttgcg gtcgtgacta cggcggcgtc ctgtggcgcg gtgattgcga tgcatgattc
     2101 gtttaccgct ctcggcggca tggtgccgat gtggctaatg caaattggtg aagtggtgtt
     2161 cggcggtgtc ggttctggtc tttacggcat gatgctgttt gtcctgctgg cggtgtttat
     2221 tgccgggctg atgattggtc gtacaccgga atatctgggt aaaaaaatag acgtacgcga
     2281 gatgaaactg actgcactgg cgattctggt tactccaaca ctggtactga tgggcgcggc
     2341 gttggcgatg atgaccgacg ccggacgtag cgccatgctt aaccctagcc cgcatggttt
     2401 tagcgaagtg ctgtacgccg tgtcgtccgc cgctaacaac aacggcagcg cctttgccgg
     2461 attaagcgcc aactctccgt tctggaactg tttactggcg ttctgcatgt ttgtcggtcg
     2521 cttcggggtg attatcccgg tgatggcaat tgccggttcg ctggtgagta aaaagagcca
     2581 acccgccagc tccggcacgc tgccaacgca cggcccgctg tttgttggcc tgttaatcgg
     2641 caccgtgttg ctggttggcg cactgacctt tatccctgcc ctggcgcttg gtccggtggc
     2701 ggaatatctc tcctgatgat attgagtgag cactgaatat gagtcgtaaa caactggcgc
     2761 tattcgaacc aacacttgtc gttcaggcgc tgaaagaagc ggtgaaaaaa ttaaacccgc
     2821 aggcgcaatg gcgcaatccg gtgatgttta tcgtctggat cggcagtctg ctgaccacct
     2881 gtattagcat cgcgatggca agcggtgcga tgcctggcaa tgcgctgttt agcgcggcaa
     2941 ttagcggctg gctgtgggtc accgtactgt tcgctaattt cgccgaggcg ctggcagaag
     3001 gccgcagtaa agcgcaggcc aacagtctta aaggggtgaa aaaaactgcc tttgcccgca
     3061 agctgcgtga gccgaaatat ggcgctgcgg cggacaaagt tcctgccgat caactgcgta
     3121 aaggtgacat cgtgctggta gaagctggcg atattatccc ctgcgatggt gaagtcattg
     3181 aaggcggcgc atcggtcgat gaaagcgcca tcaccgggga atcggcaccg gtgatccgtg
     3241 aatccggcgg cgattttgcc tccgtcaccg gcggcacgcg tattctttct gactggctgg
     3301 tgattgagtg tagcgttaac cccggtgaga catttctgga tcggatgatc gcgatggtgg
     3361 aaggcgcaca gcgacgcaaa acaccgaacg agattgccct gactatcctg ctgattgctc
     3421 tgaccatcgt ctttttactg gcaaccgcca cgctgtggcc gttttccgcg tggggcggta
     3481 atgccgtgag tgtaacggta ctggtggcgc tgctggtctg tctgatccca accactattg
     3541 gtggcctgtt gtcagcgatc ggcgtcgccg ggatgagtcg gatgctgggc gcgaatgtga
     3601 ttgccaccag cggacgtgca gttgaagcgg caggtgacgt tgacgttctg ctactggata
     3661 aaaccggcac catcacactc ggtaaccgtc aggcgtcgga gtttatcccc gcgcagggcg
     3721 tggatgaaaa aacgctggct gacgccgcac aactggcttc gctggctgat gaaacgccgg
     3781 aaggtcgcag tattgtgatc ctcgccaagc aacgttttaa cctgcgcgag cgcgatgtgc
     3841 agtcgctcca tgccaccttt gtaccgttta ctgcgcaaag ccggatgagc gggatcaaca
     3901 tcgacaaccg catgatccgt aaaggttctg tcgatgccat tcgccgccat attgaggcta
     3961 acggcggtca cttccctgcc gatgttgatc aaaaagtcga tcaggttgcg cgtcaaggag
     4021 ccacgctgct ggtggtggtg gaaggttctc gtgtgctggg cgttattgcg ctgaaagata
     4081 tcgtcaaagg cggtattaaa gagcgcttcg cccagctgcg caaatgggca ttaaaacggt
     4141 gatgattacc ggcgataacc gtctgactgc cgccgcgata gctgcggaag cgggtgtcga
     4201 tgattttctc gccgaagcga caccggaggc caagctggca ttgatccgtc agtatcaggc
     4261 ggaaggtcgt ttggtagcga tgaccggcga cagcaccaac gatgctacgg cgctggcgca
     4321 ggcagatgtc gcggtggcga tgaactccgg cacccaggcg gctaaagagg cgggcaatat
     4381 ggtggatctc gactctaacc cgaccaagtt gatcgaggtg gtgcacattg gcaaacagat
     4441 gctgatgacc cgtggctcgc tgaccacctt cagcattgcc aacgatgtgg cgaaatactt
     4501 cgccattatt ccggcggcgt tcgcggcaac atatccgcag ttaaatgcgc tgaacatcat
     4561 gcgcctgcat tcgcccgact ccgccattct cagtgcggtc attttcaacg ccttgattat
     4621 cgtctttttg attcctctgg cgttaaaagg cgtgagttat aaaccgctta ccgcttctgc
     4681 catgttgcgc cgtaacttat ggatttacgg tctgggtggg ctgctggtgc cgtttattgg
     4741 tatcaaagtc attgatttac tgctaaccgt ttgtagtctg gtgtgaggtt taccatgagt
     4801 ggattacgtc cggcattatc gacatttctc tttctgttat tgattactgg cggcgtttac
     4861 ccgctgctga ccaccgcact ggggcaatgg tggtatccct ggcaggccaa cggttcgttg
     4921 attcgtgaag gtgatacggt gcgcggttcg gcattaatcg ggcaaaattt taccggcaac
     4981 ggctattttc atggtcgccc gtcggcaaca gcagaaatgc cctataatcc acaggcttct
     5041 ggcgggagca atctggcagt cagtaacccg gagctggata aacagatagc cgcacgcgtt
     5101 gccgcattac gggccgctaa cccggacgcc agcacgaacg ttccggttga actggtgacg
     5161 gcgtcggcaa gcggactgga caataacatc accccgcaag cggcggcctg gcagatccca
     5221 cgcatcgcaa aagcgcgtaa tctcagcgtt gaacagctca cgcagctgat cgcaaaatac
     5281 agccaacaac cgctggtgaa atatatcggc cagccggtcg tcaacattgt cgaactcaat
     5341 ctggcactgg ataagcttga tgaataacga acccttacgt cccgaccccg accgtctgct
     5401 ggaacaaact gccgcgccgc atcgggggaa gctgaaagtt ttcttcagtg cctgtgcagg
     5461 cgtcgggaag acctgggcga tgctggcaga agcccagcga ctgcgggcgc aagggctgga
     5521 tattgtggtt ggtgtggtag aaacccatgg gcggaaagat accgccgcca tgcttgaagg
     5581 gctggctgtt ctgcctctga aacgccaggc gtaccgtggg cggcatatca gcgagtttga
     5641 tctcgatgcc gccctcgccc gccgtccggc gctgatctta atggacgaac tggcgcacag
     5701 taatgcgcca ggttctcgtc atcctaaacg ctggcaggat atcgaagaac tgctggaagc
     5761 tggcattgat gttttcacaa ccgtcaacgt tcagcatctg gaaagtctga atgatgtggt
     5821 cagcggcgtc accggaattc aggtacggga aaccgtgccc gatccttttt tcgatgccgc
     5881 cgacgacgtg gtgctggtgg acttgccccc ggacgatctg cgccagcggc tgaaagaagg
     5941 caaagtctat attgccgggc aggcggagcg cgccattgaa cattttttcc gcaaaggcaa
     6001 tctgatcgcc ctgcgcgaac tggcactgcg ccgtactgcc gatcgcgttg atgagcaaat
     6061 gcgcgcctgg cgggggcatc ctggcgaaga gaaagtctgg cacacgcgcg acgcgatcct
     6121 tttatgcatc ggccataaca ccggcagcga aaactggtcc gcgcggcggc gcggctgacg
     6181 tcacggctgg ggagcgtctg gcacgcggtg tatgttgaaa cccctgccct gcatcgctta
     6241 ccggagaaaa aacgtcgggc aattctcagc gccttacgtc tggcgcagga gctgggcgcg
     6301 gagacggcaa cactttctga tccagcggaa gagaaagcgg tagtgcgtta tgcccgtgaa
     6361 cataatctcg gcaagattat tctcggtcgc ccagcctcgc gccgctggtg gcgtcgggaa
     6421 acatttgctg accgactggc gcgcatcgcc cccgatctcg atcaggtgct ggtcgcgctt
     6481 gatgaaccac ccgcccgcac gattaacaac gcgccggata gccgctcatt taaagacaag
     6541 tggcgggtac agattcaggg atgcgtggtt gccgccgcgt tatgcgccgt tatcacctta
     6601 attgccatgc agtggctgat ggcgtttgat gccgccaacc tggtgatgct gtatctgctt
     6661 ggcgtggtgg tggcgctatt ttatggacgc tggccttcgg tggttgccac cgtcattaat
     6721 gtggtgagtt tcgatctctt ttttatcgcc ccacgcggca cgctcgccgt ctctgatgtg
     6781 caatatctgc tgaccttcgc ggtgatgtta accgtcgggc tggtgatcgg gaaccttact
     6841 gctggtgtgc gttatcaggc gcgggtagcc cgttaccgcg agcaacgcac acggcactta
     6901 tatgaaatgt cgaaagctct ggcggtgggc cgcagtccgc aggacatcgc cgccaccagc
     6961 gaacaattta ttgcctccac gtttcatgcc cgcagtcagg tgttgttgcc cgatgacaac
     7021 ggtaaattgc agccgttaac acatccgcaa ggaattacgc catgggacga tgccatcgcg
     7081 cagtggagtt ttgataaagg cctgcctgcg ggcgcgggca ccgacacgtt acccggtgta
     7141 ccgtaccaga ttttgccgct aaaaagcggc gagaaaacct acgggctggt ggtggtggag
     7201 ccggggaatc tgcgccagtt gatgatcccg gaacagcagc gcctgctgga gacttttacg
     7261 ctgttagtcg ccaatgccct tgagcggctg acgctaaccg ccagcgaaga acaggcgcgg
     7321 atggcaagcg aacgtgaaca gatccgcaac gccctgctgg cagcgctttc gcatgattta
     7381 cgcacgccgc ttacggtgct gtttggtcag gcagaaatct taacgctcga tctggcaagc
     7441 gaaggatcac cccacgcccg ccaggccagc gagattcgtc agcatgtgct gaacactacc
     7501 cgactggtga ataatctact ggatatggcg cgaattcagt ccggcggctt taatttgaag
     7561 aaagagtggt taacgctgga agaagtagtc ggcagcgcgc tgcaaatgcc ggaaccgggt
     7621 ttattgtcgc ccatcaatct ttctctgcca gaaccgctga ccttaatcca cgttgacggg
     7681 ccactctttg aacgggtgct gattaatctg ctggagaacg cggtgaaata tgcgggtgcg
     7741 caggccgaaa ttggtatcga tgcccacgtt gagggcgaaa atctacaact ggatgtctgg
     7801 gataacggcc ccggtcttcc gccaggccag gagcagacga tatttgataa gtttgctcgc
     7861 gggaataaag agtcggcggt gccgggtgtc gggcttgggt tggcaatttg tcgggcgatt
     7921 gtggatgtac acggcggcac cattaccgcg ttcaaccgac cggaaggtgg tgcctgtttt
     7981 cgtgttacac ttccccagca aaccgctcct gaacttgaag actttcatga ggatatgtga
     8041 caaacgttct gattgttgaa gatgaacagg ctattcgtcg ctttctgcgc acggcgctgg
     8101 agggcgacgg gatgcgcgtc tttgaggccg aaactctgca acgcggcttg ctggaagcgg
     8161 caacccgtaa gccagatttg attattctcg atctcggcct gcccgatggt gatgggattg
     8221 agtttatccg cgacctgcgc cagtggagcc cggtgccggt gattgtgctt tccgcacgca
     8281 gcgaagagag cgacaagatc gccgcgctgg atgccggagc ggatgattat ctgagtaagc
     8341 cgtttggtat tggcgaattg caggcccgtc tgcgcgtcgc tttacgccgc cactccgcca
     8401 ctccgccacc gccgcgcccg atccgctggt gaaattttcc ggtgttaccg tcgatttagc
     8461 cgcccgcgtg attcaccggg gtgaggaaga ggtgcatctc acaccaattg agttccgcct
     8521 gctggcggtg ctgctcaaca atgccggaaa agtactcacc cagcgccagc tccttaacca
     8581 ggtgtggggg ccaaacgcgg tcgaacacag tcactatttg cgtatttata tgggacatct
     8641 gcgacaaaaa ctggaacagg atccggcccg gcctcgccac ttcattactg aaaccggtat
     8701 tgggtatcgg tttatgcttt gaattgttat tcaataaatt cgcttaaata gtgattaatt
     8761 tattattaaa cctgcgccga atattattat ttggcaggtt taatttctta acgcaaatat
     8821 aaaacacgaa attacaacat ttaagtaaca aaacagcatc taatatggcg atattcataa
     8881 ttaatgattt attatctggg gtatgatcgt tttttgttga tcttcttcac agattatagc
     8941 catttcatgg atagaataac tctaccttca actgacacag caagcggtaa aggtaaatgg
     9001 aaaataataa ccgcttaatg cctcatataa ggcggacaac ccatatcatg aagtttgccc
     9061 atcgtaatag ttttgacttt catttcttta atgcccgcta gtcttctgac taaagggcac
     9121 cccaacgtac aggtctccct gactttaagc attacaggtt aatacctgta ttcctcggtg
     9181 ctcatatact gctaaccctt ttaaactcta aataattcga gtcgcagcac ttgcaacttg
     9241 aggtatgacg agtatagcca gttaccgggc tggtctgggt tattgcatct gcaaaaagca
     9301 aactactgat ttatttatca gcggtggagc tttgcttttt ttccgacgtg atcgatttct
     9361 cctttgagaa attgaggacc tgctattacc tgaaataaag agatgaataa aatgtcagaa
     9421 ttaaaaattg ccgttagccg ttcttgcccg gattgttttt ccactcatcg tgcatgcgtg
     9481 aatattgacg aaagtaatta tattgacgtt gccgccatta ttttatcagt caatgatgtt
     9541 gaacgtggaa aactcgataa aatagacgct actggctatg gcattcctgt ttttattgca
     9601 acggaaaatg aagaacgtgt accagcagaa tatctgccac gtatttctgg tgtcttcgag
     9661 cattgcgaat cacggaaaga gttttatgga cgtcagttag aaaccgctgc tagccattat
     9721 gaaacgcaac tgcgcccgcc cttcttccgc gcactggtcg attatgtcaa tcagggcaat
     9781 agcgcttttg actgcccggg tcatcaggga ggtgagtttt tccgtcgtca tccggcaggg
     9841 aatcagtccg tggagtactt cggagaaacg ctgttccgtt ccgacttatg caacgcagac
     9901 gtagcaatgg gcgatctgct gattcacgaa ggcgcgccat gcattgcaca gcaacatgcg
     9961 gcaaaagtgt ttaatgccga taaaacatac ttcgttctaa atggcacttc atcttctaac
    10021 aaagtggttt taaacgccct gctaacaccg ggtgatctgg tgctgtttga tcgcaataac
    10081 cacaaatcta accaccacgg agcgttgcta caggctggtg caacaccggt ttatctggaa
    10141 acggcacgta acccgtatgg ctttatcggt ggcattgatg cgcactgttt tgaagaaagt
    10201 tacctgcgtg agctgatcgc ggaagtcgca ccgcagcggg caaaagaggc tcgtcctttc
    10261 cgcctcgctg tgattcagtt aggcacctac gacggtacga tttataacgc ccgccaggtg
    10321 gtggataaaa ttggtcatct gtgtgactac attctgtttg actccgcatg ggttggctat
    10381 gagcaattta tcccgatgat ggctgactgt tcgccgctgt tgctggatct taatgagaac
    10441 gatccgggta ttctagttac gcaatccgta cataaacagc aggctggttt ttctcaaact
    10501 tcacaaatcc ataaaaaaga cagccacatc aaagggcaac agcgttatgt accgcacaaa
    10561 cgcatgaaca acgcctttat gatgcacgcc tccaccagcc cgttctatcc gctgtttgcc
    10621 gcactggata tcaacgccaa aatgcatgaa ggtgtcagcg gtcgtaatat gtggatggat
    10681 tgtgtggtaa atggcattaa tgcccgcaaa ctgatcctcg ataactgtca gcatattcgt
    10741 ccgttcgtac ctgaactggt ggatggtaaa ccctggcagt cgtatgaaac agcgcaaatt
    10801 gcggttgatc tgcgcttctt ccagtttgta ccaggggaac actggcattc ttttgaaggc
    10861 tatgcagaga atcaatactt tgtcgatcct tgcaaactgt tgctgacaac cccaggtatt
    10921 gatgcacgta acggcgaata tgaagcgttc ggtgtacccg cgacgattct tgctaacttc
    10981 ctgcgcgaaa atggcgtagt gccggaaaaa tgcgatctta acgccatcct cttcctgctg
    11041 actccggcag aagatatggc caaacttcag caacttgtgc cctgctggta cgcttcgaaa
    11101 aactgcttga gtccgacgcg ccattagcag aagtgctgcc ttccatctac aaacagcatg
    11161 aagagcgcta cgccggttat accctgcgtc agttgtgcca ggaaatgcat gatttgtatg
    11221 cccgccacaa cgtgaaacaa ctgcaaaaag agatgttccg caaagcgcac ttcccacacg
    11281 tcagcatgaa tccgcaagaa gccaactacg cctatttacg cggtgaagtg gagctggttc
    11341 gtctaccgga tgccgaaggt cgtatcgctg ccgaaggggc gcttccttat cctccgggtg
    11401 tgctgtgtgt tgttccgggt gaaatctggg gtggtgctgt tttgcgttac ttcagcgctc
    11461 tggaagaagg gatcaacctg ctgccaggtt ttgcaccgga gctgcagggt gtctatatcg
    11521 aagaacatga tggtcgtaag caagtttggt gctatgtcat caagcctcgt gatgcgcaaa
    11581 gcaccctgtt gaaaggggaa aaattatgag tcaggctaaa tcgaacaaaa tgggcgtcgt
    11641 tcagttaacc atactgacga tggtcaacat gatgggctcc ggtatcatca tgctgccgac
    11701 aaagcttgcc gaagttggga caatttcaat tatctcctgg ctggtgacag ccgtaggctc
    11761 aatggcaccg gcatgggcat tcgcaaaatg cggtatgttc agccgtaaat caggtggtat
    11821 gggcggttat gccgagtatg ctttcggtaa atccggtaac tttattgcga actataccta
    11881 cggcgtctca ctgctgattg ctaacgtcgc gattgctatt tcggcggttg gttacggcac
    11941 cgaactgctc ggcgcgagtt tgtcgccagt gcagattggt cttgcgacca tcggggtgct
    12001 gtggatttgt accgtggcta actttggtgg tgcgcgcatt accgggcaaa tcagtagcat
    12061 taccgtgtgg gggggcatta ttccggtggt aggcctgtgc attatcggct ggttctggtt
    12121 tagcccgacg ctttacgttg attcctggaa tccgcatcat gcaccgttct tcagtgcagt
    12181 aggttcttcc atcgctatga cgctgtgggc ttttcttggt ctggagtctg cgtgtgcgaa
    12241 tactgatgta gtggaaaacc cggaacgtaa tgtgccaatc gcggtactcg gcggtacgtt
    12301 aggtgcggcg gtgatttata tcgtctccac caacgtgatt gcagggattg tgcctaatat
    12361 ggagctggca aattcaacgg caccatttgg cctggccttc gcacagatgt ttacaccaga
    12421 agtgggtaaa gtcattatgg cgctgatggt gatgtcctgc tgcggttcgc tgcttggctg
    12481 gcagttcacc attgcccagg tgtttaaatc ttcatctgat gaaggctact tccctaaaat
    12541 tttctcccgt gtaaccaaag tggatgcacc ggtgcaggga atgttgacca ttgtgattat
    12601 ccagagcggg ttggcgctga tgacgattag cccgtcgcta aacagtcagt tcaacgtgct
    12661 ggttaacctg gccgtggtga ccaatatcat tccgtatatt ctgtcaatgg cggcattagt
    12721 cattattcag aaggtcgcta atgtgccccc ctcaaaagcg aaagttgcaa actttgttgc
    12781 ttttgttggc gcgatgtata gcttttatgc gctgtactca tccggggaag aagccatgct
    12841 gtacggttcc atcgtgacct tccttggttg gacactgtat ggtctggtat caccacgctt
    12901 tgaactgaaa aataaacacg gttaaaaaag caaagtaaaa aagggcgatc ttgcgaccgc
    12961 tcttttttta ttaaatgtgt ttacgcgttt ttcagaactt cgctaacaat ctcaaccgct
    13021 tctttctcaa tctgcttgcg atgttcttca ccgaggaagc tttcgcagta gatcttatat
    13081 gcgtcttccg tgcctgacgg acgcgcggcg aaccagccgt tgtcagtcat cactttcaga
    13141 ccgccaatag aagcaccgtt gcccggagca gcagtcaggc gcgcggtgat cgggtcacct
    13201 gccagggtgc tggcgctcac catttccgga gacagcttag acagcgccgc tttttgtgcg
    13261 gaagtcgcag ctgcctgcaa acggttgtag ctcggcgcac caaagcgttt tgccagttcg
    13321 ttgtagtgtt cctgcgggtt cttaccggtg acagcggtga tttccgccgc cagcagacac
    13381 ataatgatgc cgtctttgtc ggtggaccac ggcgtgccgt cgaaacgcag gaaggaagcc
    13441 cctgcgctct cttcgccgcc aaagccgaag ctgccgtcga acagaccatc aacaaaccat
    13501 ttgaaaccta ccggtacttc taccagctta cggcccaagt cattgaccac acggtcgatc
    13561 atcgcagaag aaaccagcgt tttaccgaca gcaacatctt tgccccactg cggacgatgc
    13621 tggaacaggt agttgatcgc caccgccagg tagtggttcg gattcatcaa acctgccgga
    13681 gtgacgatac cgtgacggtc ataatccggg tcgttagcaa acgccagatc gaacttatca
    13741 cgcagtgcca gcagacccgc catcgcacac tcggaggagc agtccatacg gatcgcaccg
    13801 tctttatcga ggtgcataaa gcggaaggtt tgatcgacct gatcgttaac gatggtcagg
    13861 ttgaggttgt aatactcgcc aatgcgcttc cagtattcga taccggaacc gcccaacgga
    13921 tcaacgccca gcgtcaggcc cgctttctga atcgccgcca tatcaacgat atcggtcagc
    13981 ccttccacga acggctgcac cagatcctgc tctttcacat agccggatgc catcgcttcg
    14041 tcgagggaga tacgcttcac gcctttcagg ccatcggcca tcagtgcgtt ggccttgtct
    14101 tccaccactt tggtgacgtt agtatcagcc gggccaccat ttggcggatt gtacttgata
    14161 ccaccatctt ccggcgggtt atgggacggt gtaatcacga taccgtctgc cagcgggcca
    14221 ccttttttat tgtgaaccag gatggcattg gaaacggcag gcgtcggagt gaagccattg
    14281 ttttcctgca caatgacatc aacgccgttc gctgccagca cttccagtac ggaaatgaac
    14341 gcaggctcgg acagggcgtg agtatcttta cccacatagc aagggccagt gatgccgttt
    14401 ttcgcacgtt cttcagcaat tgcctgagcg atcgccagaa tgtgcggctc gttaaagctg
    14461 tggcgcgctg cactgccacg gtgaccggaa gtaccgaatt tcaccgcgtg ctccgcattc
    14521 cctgcttctg gtttcagtac ataatattgc gccgtcagtt gggcgacgtt aatcaaatca
    14581 ctctgttgtg caggttggcc tgcacgattg tggattgcca ttgctttgtc ctttgtctgc
    14641 aacgttttag atagttccgc aaaccttctc aatcaattcc gccgggaatt gcatcgactg
    14701 catgatgtgt tcgatcatgc tgcatttacg gccggtgttg gtgttggtga tcacccaata
    14761 cggcgtgcct ggcacatgtt tcggcttggt ctgattacca tttttcagca gcgtttgttc
    14821 atctgccgca aagtacacgc gtgtacgacc gtgcaacgat tccgttgctt cggcaaacgc
    14881 ctgggcgtca agagaatata gtgtagacaa cagcagcata aagcgattga ccgctcgctt
    14941 ttgctctgcg tattcgtccg aaagcagaag ttcacgcatt gcgcgaacct tgtctttaat
    15001 cgttttgacc ggcttcgctt cgacgatagc aggtgacgca acgcgaacct ctttcgtcac
    15061 cggagcagca ggctgtgatg cggcggaaaa tttcaacata cgccgtaaaa tgtcggatgc
    15121 gctctcgccg atatgcttag tgtggctggc aatatagctg tagagttcat catcaacttc
    15181 aatcgttttc atcttaatcc agtgcagtgt cttagctgaa tacaagtcgc caagagtata
    15241 tgaataaatc ccggcagcgg atagcgtcaa gcctggcttg cagaaaaact cggaataaac
    15301 cttatttgtt gcagccctgt ggcacaatgg tcaacataat accctaaccc gacaacgcga
    15361 aaaaaagaac tttgccatga aattaaatat ccgcgcgcaa actgcacaaa accagcacaa
    15421 taattctccc atcgttcttg tccatggtct gtttggtagc ctcgacaacc ttggcgtact
    15481 ggctcgcgat ctggtaaacg atcacaatat catccaggtt gatatgcgta accacggtct
    15541 ttcaccgaga gagccggtaa tgaattaccc ggcgatggcg caggatcttg ttgataccct
    15601 ggatgcactg cagatcgaca aagcaacatt tatcggtcac tccatgggcg gtaaagcggt
    15661 aatggcactt actgcactgg cccccgatcg catcgataaa ctggtggcga tcgatatcgc
    15721 accggtcgac tatcacgtac gccgtcatga tgagattttc gcagctatca acgcggtcag
    15781 tgaatcggac gcacaaactc gccagcaagc agcagcaata atgcgccagc atcttaatga
    15841 agaaggggtg attcagtttc tgttgaaatc ttttgttgac ggggagtggc gctttaacgt
    15901 gccggtattg tgggatcagt atccgcatat tgtgggttgg gagaaaatcc cggcatggga
    15961 tcaccctgcc ctgtttatcc ctggcggcaa ttctccgtat gttagcgagc agtaccgtga
    16021 tgatttactg gcacaatttc cacaggcacg ggcgcatgtg attgcaggcg cgggtcactg
    16081 ggtccatgct gaaaaaccgg atgcggtatt acgcgctatc cgtcgctatc tcaatgatta
    16141 acgcaactga ttaaaaaccc agcgtccgcg tcgctttccg cgcgtgggcg ttggcgtgac
    16201 tcatcgcgct gatgtattat ggcgcgctat ccatccgggc agaggcctgg ctgattgttt
    16261 cccccgaagt caccaagatc atggccaaag aacaaacgga ccgtacgaca ttagatctgt
    16321 tcgcgcacga gcgtcgaccg ggacgaccga aaactaatcc gctttcgcgc gatgaacagc
    16381 tgcgtattaa taaacgcaac cagctaaaac gcgacaaagt acgtggcctt aagcgtgtcg
    16441 aactgaagct gaacgcggaa gctgtcgagg cgctgaacga gctggcggaa tcgcgtaata
    16501 tgagccgtag cgaactgatc gaagagatgt taatgcagca actggcggcg ctgcgtagtc
    16561 agggcatcgt ttaaatttcc actttcatgt agcacagtgt gcagtcctgc tcgtttgctg
    16621 attccgcaag actgcctgtt ctgctatgat tgcctttatc cgtgggcaat tttccacccc
    16681 catttcaata agtttcaaga ggttatttca ctcatggcta tcactggcat ctttttcggc
    16741 agcgacaccg gtaataccga aaatatcgca aaaatgattc aaaaacagct tggtaaagac
    16801 gttgccgatg tccatgacat tgcaaaaagc agcaaagaag atctggaagc ttacgacatt
    16861 ctgctgctgg gcatcccaac ctggtattac ggcgaagcgc agtgtgactg ggatgacttc
    16921 ttcccgactc tcgaagagat tgatttcaat ggtaaactgg ttgcgctgtt tggttgtggt
    16981 gaccaggaag attacgccga atatttctgc gacgcattgg gcaccatccg cgacatcatt
    17041 gaaccgcgcg gtgcaaccat cgttggtcac tggccaactg cgggctatca tttcgaagca
    17101 tcaaaaggtc tggcagatga cgaccacttt gtcggtctgg ctatcgacga agaccgtcag
    17161 ccggaactga ccgctgaacg tgtagaaaaa tgggttaaac agatttctga agagttgcat
    17221 ctcgacgaaa ttcttaatgc ctgatgtgat gcggcgtaga ctcatgtcta cgccgtatta
    17281 atagataatg ccaatcaaaa taattgctac aaatttgtaa cttttgctgt tgtacctata
    17341 caatgtccca gtgttcaagt ggccttgccg ttgtaaatgt aagctgtgcc acgtttttat
    17401 taacaatatt tgccagggac ttgtggtttt catttaggcg tggcaattct ataatgatac
    17461 gcattatctc aagagcaaat tctgtcactt cttctaatga agtgaaccgc ttagtaacag
    17521 gacagattcc gcatgactga taacaatacc gccctaaaga aagctggcct gaaagtaacg
    17581 cttcctcgtt taaaaatcct ggaagttctt caggagccgg acaaccatca cgtcagtgcg
    17641 gaagatttat acaaacgtct gatcgatatg ggtgaagaaa ttggtctggc tacggtatat
    17701 cgcgtactga accagtttga cgacgctggt atcgtcaccc gccacaattt tgaaggcggt
    17761 aaatccgtat ttgaactgac acagcaacat caccacgatc acctgatttg cctcgactgc
    17821 ggcaaggtta tcgaatttag tgatgattcc atcgaagcgc gtcagcgtga aattgccgca
    17881 aaacatggca ttcgcctgac taaccacagt ctctatcttt acggtcactg tgccgaaggc
    17941 gattgccgcg aagatgagca cgcgcacgaa ggcaaataag ccagcctgaa cgagaaaagc
    18001 caacctgcgg gttggctttt ttatacaagg gaaataagaa aagcgagagt tacagctctc
    18061 acttatttgt tattactgcg tatttcctgc cagaccttat cacaatcggc tttcaccgcc
    18121 tgatcatttc cggtttgcgt tgcacgcaga cactgctgat aatccagtac gcgtacactt
    18181 tcctggtcgg caaactgctg atgttgtttc tctttcttca gcacgtttaa cacgctctgg
    18241 caggcttcaa ttttttctgg cgaaccttgc gcggtgttga tacaggcgct gtatgcctct
    18301 ttcagacggc tgtcttcctg tggtgcggtt gattgcgcac aagctaccag ccccgatgcc
    18361 atcatggcga tgagaatcag ttttttcatt gaatgcggca ggcacaaaac ctgccgcgtc
    18421 gggcatcaga agatggtgaa tggtgcgatt accataaatt tcacgtcacg ctcatcctgg
    18481 aagatgttac cgtaaccgcc gccccagctt gggatgtcgg agtggttgtc gtattcggtg
    18541 aagtgcagtt tgaacatcgt gcctttggcg cgaccgtcct gaatggtgta gaccgcatcc
    18601 aggctgtagg cagactcttc aatagtccgg tttttgtcgt agtacgcatc cggattgctc
    18661 tgccaggtcg caggtttagc atcccatgcg taaacgtagg aagcgccgat ggcgaagcct
    18721 ggaagattcc agtttttcag gtcatacatc gcactgaaga agaccgcttt ttcgccgtta
    18781 gcgttgaagt cggaacggtt atcccaccag atatccaggc ggccgttgga ggaagcgtag
    18841 gttggggtca tacgttgcag gaagtatccc tgctgactgt cagccttaat ccaggtgcct
    18901 tcgaggcgca aatccactac gtcagccgcc cggtaaccaa aggtcaacgc ttgcagccag
    18961 gcggtgccgt cataaagatc gttgacgctg cgatcgtcaa ctttatcgcg ggtaccatag
    19021 aactggtagc tggtggttaa cgggctaccg gcgatatcaa atttgtagct ggctttggca
    19081 aaatattgat cgatataccc ttccgcctga ccaaatgccg cttccagtac gaagttattt
    19141 ttgaagtcgt atttcgcacc gatggagtgc agataatcaa ctttggtggt tttatcgttc
    19201 tgataaaact catccatttc cagatgccac ggcgctttgt attcgttggt ccacatgtag
    19261 gagaaactca aggcaccagc atcgccgtaa tcaaaattcg ccccggcttc cgccccctga
    19321 taagtacccg gcataaagct ccagtgcggc gctaacagcg tttgaccggt tggctgaatg
    19381 taacctgccc tcgcccaaac cggaccgtat ttaaatttgg ccgcagcttt atacaggctt
    19441 ataccgcttt tgtcgccgga ccagtcttcg tcataggctt tattactttt tgaaaacgcg
    19501 atttcgttcg ggtgggagct gtcgccgttt tccgccattt caatcgccgt aaacgcagca
    19561 atatcaaggc cgaacatatc agcagcataa ccggactgaa aatcgaggtt ggcattccag
    19621 gtggagtgag aaaggttggt tttgtatttg tcgccgtcgg taacatcttt acggtcgcgt
    19681 tcacgctgcc agtaatagat accgccggtt aaggttgaat cgtcgatgaa tccttcggcg
    19741 cgagcctccg gcattgccat aaagcccgac attgctgtaa caccggcaat agccagcgcc
    19801 agcgtactac gtttgccact aaacgtacgc atgggttaat cctctttgac gtataaattg
    19861 ctgcaccaaa ggtgaacagc gaaaaaaaat aaaaattaaa aaagtagcgt tgagaaaagt
    19921 ctcgctgact acaggaacag actatttttc gcgacgcgaa ttatcaatct ctttctgtaa
    19981 ccagaatgta agaacatgag caaccgcaca tattttgatg atttttgtta caaggcttaa
    20041 gtttgcggtt gaaaggcttg cagaagaata aaaacgtgaa attccagcaa gatgaaagcg
    20101 gttacatttt atttaattat gttttgtgat tttaaattta ttttatggat aaattgcgaa
    20161 gcggaattaa ttcgcgaaga aaagcagaaa aaacgccgca gagcgcggcg tttgggattg
    20221 tttgctattt cttgccggat gcgg
//
LOCUS       DAEBXN010000078        20130 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.078, whole genome shotgun sequence.
ACCESSION   DAEBXN010000078 DAEBXN010000000
VERSION     DAEBXN010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20130
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.078"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            88..864
                     /gene="exuR"
                     /locus_tag="KJL99_002808"
     CDS             88..864
                     /gene="exuR"
                     /locus_tag="KJL99_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HBD4437474.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1209..1871
                     /locus_tag="KJL99_002809"
     CDS             1209..1871
                     /locus_tag="KJL99_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBD4437475.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1875..2258
                     /gene="mzrA"
                     /locus_tag="KJL99_002810"
     CDS             1875..2258
                     /gene="mzrA"
                     /locus_tag="KJL99_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HBD4437476.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2405..2759
                     /locus_tag="KJL99_002811"
                     /pseudo
     CDS             2405..2759
                     /locus_tag="KJL99_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
     gene            2797..3102
                     /locus_tag="KJL99_002812"
     CDS             2797..3102
                     /locus_tag="KJL99_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HBD4437477.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3105..3509
                     /locus_tag="KJL99_002813"
     CDS             3105..3509
                     /locus_tag="KJL99_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBD4437478.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3499..3798
                     /locus_tag="KJL99_002814"
     CDS             3499..3798
                     /locus_tag="KJL99_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HBD4437479.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDISASSREWLEATGAYDRRWN
                     MLLSLRSWALVGSRVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3984..4280
                     /locus_tag="KJL99_002815"
     CDS             3984..4280
                     /locus_tag="KJL99_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HBD4437480.1"
                     /translation="MKKLEDVGVLVAHILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLLTAFLFHSNFAEGVNSLMFMKNLTISGGFLLLAITGPGAYSIGRLLNKKW"
     gene            4350..5336
                     /gene="yqjG"
                     /locus_tag="KJL99_002816"
     CDS             4350..5336
                     /gene="yqjG"
                     /locus_tag="KJL99_002816"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HBD4437481.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5629..5997
                     /locus_tag="KJL99_002817"
     CDS             5629..5997
                     /locus_tag="KJL99_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HBD4437482.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTIIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
     gene            complement(6204..7100)
                     /gene="yhaJ"
                     /locus_tag="KJL99_002818"
     CDS             complement(6204..7100)
                     /gene="yhaJ"
                     /locus_tag="KJL99_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HBD4437483.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFAGK"
     gene            7205..7906
                     /locus_tag="KJL99_002819"
     CDS             7205..7906
                     /locus_tag="KJL99_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HBD4437484.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDTCPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            7929..8093
                     /locus_tag="KJL99_002820"
     CDS             7929..8093
                     /locus_tag="KJL99_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4437485.1"
                     /translation="MSKKSTKKRQPAKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(8305..9615)
                     /locus_tag="KJL99_002821"
     CDS             complement(8305..9615)
                     /locus_tag="KJL99_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708914.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HBD4437486.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AITDAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTHQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITAIMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(9643..10974)
                     /locus_tag="KJL99_002822"
     CDS             complement(9643..10974)
                     /locus_tag="KJL99_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HBD4437487.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTDMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSLFLAFS"
     gene            complement(11249..12613)
                     /gene="tdcG"
                     /locus_tag="KJL99_002823"
     CDS             complement(11249..12613)
                     /gene="tdcG"
                     /locus_tag="KJL99_002823"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HBD4437488.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPCRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(12685..13074)
                     /locus_tag="KJL99_002824"
     CDS             complement(12685..13074)
                     /locus_tag="KJL99_002824"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HBD4437489.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(13088..15382)
                     /gene="tdcE"
                     /locus_tag="KJL99_002825"
     CDS             complement(13088..15382)
                     /gene="tdcE"
                     /locus_tag="KJL99_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HBD4437490.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKSEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(15416..16624)
                     /gene="tdcD"
                     /locus_tag="KJL99_002826"
     CDS             complement(15416..16624)
                     /gene="tdcD"
                     /locus_tag="KJL99_002826"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HBD4437491.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENAHVICVVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(16650..17981)
                     /gene="tdcC"
                     /locus_tag="KJL99_002827"
     CDS             complement(16650..17981)
                     /gene="tdcC"
                     /locus_tag="KJL99_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HBD4437492.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(18003..18992)
                     /gene="tdcB"
                     /locus_tag="KJL99_002828"
     CDS             complement(18003..18992)
                     /gene="tdcB"
                     /locus_tag="KJL99_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HBD4437493.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(19091..20029)
                     /gene="tdcA"
                     /locus_tag="KJL99_002829"
     CDS             complement(19091..20029)
                     /gene="tdcA"
                     /locus_tag="KJL99_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HBD4437494.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
BASE COUNT         5208 a         5273 c         4811 g         4838 t
ORIGIN      
        1 aatcatcaaa tttatatatg accaatggca tacccatgaa taaagtggta tgataacttc
       61 gtccgcattc cactccggga gacgcatatg gaaatcactg aaccacgccg tttgtatcaa
      121 caacttgctg ctgacctgaa agagcgcatt gaacagggcg tctatctggt gggtgataaa
      181 ctgcctgccg aacgctttat tgccgatgaa aagaacgtca gccgaacggt agttcgtgaa
      241 gccatcatca tgctggaagt tgaaggctat gtggaggtgc gtaaaggttc aggtattcat
      301 gtggtttcca accagccgcg tcatcagcag gcggctgaca ataatatgga attcgccaat
      361 tacggtccgt ttgagttgct tcaggctcgc cagctcatcg aaagtaatat tgccgagttc
      421 gcggcaactc aggtaacgaa acaagacatc atgaaactga tggccattca ggaacaggcg
      481 cgcggcgaac aatgcttccg tgattccgag tgggatttac agttccacat tcaggtagcc
      541 ctggcgacgc agaactccgc cctggcggcc atcgttgaaa aaatgtggac ccagcgtagc
      601 cataacccgt actggaaaaa actacacgaa cacattgatt cccgtaccgt tgataactgg
      661 tgtgatgacc acgatcaaat tctcaaggcg ttaattcgca aagatcctca tgctgctaag
      721 ctggcaatgt ggcagcatct ggaaaacacc aaaatcatgt tatttaacga aaccagcgac
      781 gatttcgagt tcaatgccga ccgctatctg ttcgccgaaa acccggtagt gcatctcgat
      841 acggccacca gcggcagcaa atgaaattcc tgtccgacag gcgctgcaat gcgcctgttt
      901 gtctcgtcgg taagcgaaaa gtataaagtg tcagcctgtg taaatcctct cgccaccctc
      961 ccctgcaatc agcaaaatca gactccacgg acatgtaatt ttgataacga acaacgttga
     1021 cctttgttac aattagattc aatttgaatt tatgcttttg aatgctttct tatctcacgt
     1081 tttaacaggg aatagttcag gctgtgttga tgtatcaaac ccgcagaaca taccaaaaca
     1141 gcaataacat tgcggtagtg catcttttaa aaccagcgtg gcgttaaccg attcaccagg
     1201 aataatgaat ggaacttttg acccaattgc tgcaagccct gtgggcgcag gattttgaaa
     1261 ccctggccaa tccatcgatg attggcatgt tgtattttgt cttgtttgta attttgttcc
     1321 ttgaaaatgg cttgcttccg gcggcctttt taccgggcga cagtttactg gtattggtcg
     1381 gcgtgttgat tgcgaaaggc gcgatgggtt acccgcaaac gattctgctg ctgaccgttg
     1441 ccgccagcct cggttgctgg gtcagctata ttcaggggcg atggctgggc aatacccgca
     1501 ccgtacaaaa ctggctatct catttacccg cgcattatca tcaacgcgca caccatcttt
     1561 ttcataaaca cggtttatcg gcgctgttaa ttggtcgctt tattgcattt gtcagaacac
     1621 tgctgccgac gattgccggg ttatcagggc tgaataacgc gcgctttcag ttcttcaact
     1681 ggatgagcgg tctgctgtgg gtattgatcc tgacaactct gggttacatg ctcggcaaaa
     1741 cgccggtatt tttaaagtac gaggaccagc tgatgtcatg cctgatgctg ctcccggtgg
     1801 tgctgctggt gtttggcctg gcaggttctc tggtcgtgtt atggaaaaag aaatatggaa
     1861 atcgggggta agggatgcaa atacctcgca tgtcgcttcg ccagctagcc tggtccggca
     1921 ctgttttact tctggtcggc acgctgttgc tggcctggtc cgcggttcgc cagcaagagt
     1981 ctacgctggc gattcgtgcc gttcatcaag gcacaacgat gccagacggt ttttcgatct
     2041 ggcatcacct tgacgctcat ggcattcctt tcaaaagtat cacccccaaa aacgacactc
     2101 tgttaattac atttgactcc agcgaccaga gcgccgccgc aaaagcggtc ctcgacagaa
     2161 cattgcccca tggctacatc attgcgcagc aggacaataa cagtcaggct atgcagtggc
     2221 tgacccggtt acgggataac tctcatcgct tcggataact tcctgttatc cgaaacatat
     2281 cactcacttt ggtgatttca ccgtaactgt ctatgattaa tgaggcggtg gttaacacta
     2341 ccgcttcgtt gtctggacaa tcgttccttt gtaataggtc cgaatcacaa tggaaggttc
     2401 aagaatgaaa taccgcatcg ctttagctgt ttctctcttt gctcttagtg ccggtagtta
     2461 tgccactacc ctgtgtcagg aaaaggagca aaatatcctt aaggagatca gctatgccga
     2521 aaaacaccaa aaccagaatc gtattgacgg tctgaataaa gccctgagtg aagtccgggc
     2581 caactgttca gatagccagc tgcgtgccga tcatcacaag aaaatcgcaa agcagaaaga
     2641 tgaggtggcg gaacgccagc aagatttagc cgaggcgaag caaaaaggcg atgccgatag
     2701 aacggaaact ggcagaagcg caggaagagc tgaaaaagct ggaagcgcgc gactactaac
     2761 tcacaatagt cactacttac tcacctggag aaaactatgt cgaaagaaca cactacggaa
     2821 catctgcgtg ctgagttgaa atccctttcc gatacgctgg aagaggtgct tagctcatct
     2881 ggcgagaagt cgaaagaaga gttgagtaag attcgtagca aagcggagca ggcactgaaa
     2941 cagagccgtt atcgcctggg tgaaaccggt gatgccatcg ccaaacaaac ccgtgtcgcg
     3001 gcggcgcgtg ctgatgagta tgtgcgtgaa aatccgtgga ccggcgtggg aattggcgct
     3061 gcaatcggtg tagtgctcgg cgttctgctg tcgcgtcgtt aattatggcg gacactcatc
     3121 acgcgcaagg gcccggtaaa agcgttctgg gcatcgggca gcgaattgtt tccatcatgg
     3181 ttgaaatggt ggaaacacgt ctgcggctgg cggtggtgga gctggaagag gaaaaagcta
     3241 atctctttca acttttactg atgctgggcc tgacgatgct tttcgctgca tttggtctta
     3301 tgagcctgat ggtgctaatt atttgggcgg ttgatccgca atatcgcctg aatgcgatga
     3361 ttgccaccac cgtggtgttg ctgctactgg cactgattgg cggtatctgg acgttacgta
     3421 aatcgcgtaa atccaccttg ctgcgccata cacgccatga gctggcaaac gatcggcaac
     3481 tgctcgagga ggagtcccgt gagcagtaaa gtcgaacgtg aacgacgtaa ggcgcaactg
     3541 cttagccaga tccagcaaca acggctggat atttccgcca gtagtcgtga atggctggag
     3601 gcaacaggcg cttacgatcg tcgctggaat atgctgctaa gtctgcgctc ctgggcgctg
     3661 gttggcagta gagtgatggc gatctggacg attcgccatc ctaatatgct ggtccgctgg
     3721 gccagacgcg gttttggcgt atggagcgcc tggcgtctgg ttaaaacgac cctcaagcag
     3781 caacagcttc gcggttaact tccctctggc cggagcaatt ccggccttat ccatcaaatt
     3841 ttttgaatat ttttgacagt tttccttgct aacaatcatc attcaccacg tttatgattc
     3901 tctccatcga cagcaacgac gctaatagcg cgcccttgca caaaaaaaca atcagcagcc
     3961 tgagtggcgc agtggagagt atgatgaaaa aattagaaga tgttggtgta ctggtagcgc
     4021 acattttaat gccgattctg tttattaccg caggctgggg aaaaatcacc gcttactctg
     4081 gtacccaaca atatatggaa gcaatgggtg tcccgggttt tatgctgctg acggcatttt
     4141 tatttcacag caactttgct gaaggcgtca actcgctgat gttcatgaaa aacctgacaa
     4201 tttctggcgg attcctgctg ctggcaatta ccggtccggg cgcgtatagc atcggccgcc
     4261 tgctgaataa aaagtggtaa gcacgcgcta tacttaacga taaaaaacga ggaggaagct
     4321 cctcgttttt gctattggag gagagaaaaa tgggtcaact gattgacggc gtctggcatg
     4381 acacctggta cgataccaaa tctaccggcg gtaaatttca acgttcagct tccgcatttc
     4441 gtaactggct cactgccgat ggcgcacctg gccccactgg cacaggcggt tttatcgcag
     4501 agaaagatcg ttatcatctc tatgtttcac tcgcctgccc gtgggcgcac cgcacgctga
     4561 tcatgcgcaa actcaaagga ctggaaccgt ttatttccgt ttccgtagtg aacccgctga
     4621 tgctggaaaa cggctggacc tttgatgaca gttttccggg agcaaccgga gatacgctct
     4681 atcaacatga atttctgtat cagctttatc tccacgccga tccacactac agcggacgag
     4741 ttactgttcc cgtgctgtgg gacaaaaaga accacaccat cgtcagcaac gaatcagcag
     4801 aaatcatccg catgttcaat accgcgtttg atgcgctggg cgcaaaagcg ggtgattact
     4861 acccaccagc cctgcaaacg aaaattgacg aacttaacgg ctggatttat gacaccgtta
     4921 acaacggcgt gtataaagct ggttttgcca ccagccagca agcctacgac gaggcggtgg
     4981 cgaaagtgtt tgaatcgctg gcgcgactgg agcagatttt aggccagcat cgttacctga
     5041 ccggcaacca gctaaccgaa gccgatattc gcctgtggac tacgctggtg cgttttgatc
     5101 cagtgtatgt gacccacttc aagtgtgata agcaccgcat cagcgattac ctgaatctgt
     5161 atggcttcct gcgcgatatc taccagatgc cggggatcgc cgaaactgtc aatttcgatc
     5221 acatccgtaa tcattacttc cgcagccata agaccatcaa ccctacgggg attatttcaa
     5281 ttggtccgtg gcaggatctc gatgaaccgc atggacgaga tgttcgcttc ggttaaaaaa
     5341 aggcacctta cgaggtgcct ctgataatta gctaagtgca tgttgttcat gtcggatgcg
     5401 gcgtgaacgc cttatcaaac ctacaaagcg tttatattca atatgttaca atgaccgcgt
     5461 aggccagata agcgtagcgc atctggcgat tttgcgcttg ccatcagtct tagcgcgcct
     5521 tcacggcact ttattaattc cttgaacgaa tatttactgc catttcattc acgtctattc
     5581 ttaatttgct gctttaaaaa caagtgattg agcaaaattg aggcaaaaat ggactggtat
     5641 ctgaaagtac taaaaaatta tgtcggtttc cggggtcgtg cgcggcgcaa agagtactgg
     5701 atgtttattc tggtcaacat catctttacg ttcgtgctgg ggctgctgga taaaatgtta
     5761 ggctggcaac gcgctggtgg cgaaggcatc ctgacgataa tctacggtat tctggtgttt
     5821 ttaccgtggt gggcggttca gttccgccgc ctgcacgaca ccgaccgctc ggcgtggtgg
     5881 gcactgctat tcttaatccc gtttatcggc tggcttatta tcatcgtctt taactgccag
     5941 gcgggtacgc caggcgaaaa ccgctttgga ccagatccaa aactggaaca agaataatga
     6001 ttaagcacct tgtaaaaggt gcttcaaatt gttgacaaag tgcgctttgt ttatgccgga
     6061 tgcggcgtaa acgccttatc cagcctacaa attgccaaaa ttcaatatat tgcagtgact
     6121 gcgtaggcct gataagcgta gcgcatcagg cagttttgcg tttgtcagca gtcagaagca
     6181 ccttgtgaaa ggtgcttaat agattatttt ccagcgaaca actttggaat ttcccgcaga
     6241 caccaggatt tcgcttcccc catactgtca cgacgccagg ccataataat atcgatctcg
     6301 ctggtcgatt ccgggctgac gacacgcaac cgcccttccg caatatcttt ttcgaccatc
     6361 ggatacggca tcgtcgccac gccaagcccc gccagtaatg cctgacgttt atcttcaatc
     6421 gtgctcaccg ttaagcgcgg ctgtttgtcc agcagctgta cggtcaacac cgggcgctca
     6481 cgagcggtat ccgctaccgc aattccacga tatttcacac gcgtcacttc agataacggt
     6541 tccggctcct gatgaatcgg gtgatcaggc gcggcaacgt agacgttcat taacgtatag
     6601 agcttgcgcg agttgatctc cgacgaggaa cgaaaatgca tatccggcgc gataacgata
     6661 tccgcccgcc cctgctccag ccgttcccac gcccccgcca gcacttctgt gatgattgcc
     6721 agttgggtat tggcttttgc cgccagtttg tcgattaacg ggaaaaaggc aggtgtcggt
     6781 accagcgctt cggtcacaat ggtgagatgc gtttcccaac cgcgcgcgag agcttccgca
     6841 tcagtagtca gtttatctgc ggcttccagc aaaacgcgcc ctcgctccag caacatcctc
     6901 ccgacattgg tgaatttggt gcgatggccc gagcggtcaa acagcaccac atccagctct
     6961 tcttccagtt tttgcatggt gtagctaagt gcggaaggca cgcgtcccag ctcatccgct
     7021 gccgccgcaa aactgccccg gcgatcgatc gcatccataa cccgtagtgc ttccagcgtt
     7081 aatgcccttt ctttggccat ttcgttctca ttcaggaaat ttgaacatac cgggcagaat
     7141 atctggctaa caatgtagcg tccagcccct taccataaaa ggaagtaaag agaggtcaag
     7201 aattatgatt actacccgaa ctgccaggca gtgtggacaa gcagactacg gatggttgca
     7261 ggcccggtat actttttcct ttggacacta cttcgacccg aaattgttag gctatgcctc
     7321 cctgcgtgtg cttaaccagg aagtgctggc cccaggtgcc gcctttcagc cgcgaaccta
     7381 tcccaaagtc gatattttaa atgtgattct ggatggagaa gcagagtatc gcgacagcga
     7441 aggcaatcat gttcaggcca gcgccggaga ggcgttgctg ctctctaccc agccgggtgt
     7501 cagctatagc gaacacaatc tcagcaaaga caaaccgtta acgcgaatgc agctttggct
     7561 ggatacctgc ccgcagcgag agaatccgct gattcaaaag ctggcgctta atatggacaa
     7621 gcagcaatta atcgcctcgc cagagggtgc gatgggaagc ctgcaattac gccaacaagt
     7681 gtggctgcac catatcgtgc tcgacaaagg cgaaagtgcg aatttccagt tgcatgggcc
     7741 acgcgcgtat ttgcaatcga ttcacgggaa atttcatgcg cttacgcatc atgaagagaa
     7801 agcggcgcta acctgcggtg atggggcgtt tattcgtgac gaggctaaca ttacgctggt
     7861 tgccgattcc ccactgcgcg ctttgctgat agatttgcct gtctagttgt ttttacaggg
     7921 agatgatgat gagtaaaaaa tcgaccaaaa agcgccagcc agcgaagccc gtggtggcga
     7981 aagaacctgc tcgcaccgcc aaaaattttg gctatgaaga gatgttgagc gagctggaag
     8041 ctatcgtcgc ggatgctgaa acgcgtttag ccgaggatga agctaccgcg taaagacagg
     8101 ttgccggatg cggcgtaaac gccttatccg gcctacaaaa cacaacaaca tcaacaaatt
     8161 aaacaccccg taggccggat aagatgcgcc agcatcgcat ccggcatcag gtacaaaaca
     8221 caacaacatc aacaagttga gcaccccgta ggccggataa ggtgcgccag catcgcatcc
     8281 ggcacaatac tcaaaacctg gcgtttatct ggccttgctc gccataatct cgataatctg
     8341 ccgatccgtt tgctgcatcg aatggcttgc taacgcacac aggttggcaa tcgactgctc
     8401 aacatcatgc gccacgatcc cttcattgcc ggtcacggcg gtatcatcca gcgccattaa
     8461 caccgctttc cacgcagccg aagcactggt cgaaaccttc atcgcgcagc tgttcgacgc
     8521 accatcgcaa atcatgccgc tgacatcgcc gatcatactg ctgatcgcca tcgagatagt
     8581 ttcataacgc ccatccacca gccatgccat cccggcggcg gcccccattg ctgcggtcgt
     8641 tgcggcacaa agtgcagaca aacgcggtaa ctggttatgg atgtaaattg cgctcaaatg
     8701 agaaagcatc agcgcacgcg ccagtcgttc atcatccgct ccgaagtgtt ctgctacaac
     8761 caccacaggc attattgcag tgatcccctg gttacccgag ccggagttac tcatagccgg
     8821 aagcgtagcg ccgcccatac gcgcatcgga tgccgcgctg gtacgaatca caatggatga
     8881 agagagatct ttcgccagca aaccgcgctc gcactgtttt tccagcgtcg cgccaatatg
     8941 cagcccccac ttaccgctca aaccttcctg cgataacgca caatttagct tcgcggaatc
     9001 gagaataaag cggatcgccg caaatgggac ttcattgacg aacttcagga tctcagccag
     9061 cgtcgttctg gaaagcaccg tcagcggcga ctcttgctcg ccctctgcca cacacgcctg
     9121 gtgggtaaac accacaccat tgtgcgtctc aatatgcaca atgttggtat gcccgccgac
     9181 gatggtgaca cacgcccact tctcaccgtt ccagactttg gcgcgtgaga agaggatttc
     9241 attgcaaggt tcctggatct taacagagac tttccccgcc gccagcagtg ctttggcatc
     9301 ggtaattgcc tgcgcagttg cgtctttcag cacttccagc ccggcgttgg catttccacc
     9361 taacgccccc agcgccgccg caatcggcag ccccaccatt cccgtgccgg gaacggtgac
     9421 gcccagaccg ttcttcatca gatttggcga aacccaggct tctacacgtt caaccggacc
     9481 ttccagttct gccgcagcaa ccgccgccgc cagcgccagt gaaatcggtt cagtacatcc
     9541 cagcgccggt tttacttcct cctgaacggc gaggatgtaa cgctgccata acggatttaa
     9601 agtcgaatca aacatattaa aaaccttaaa atttcaggta aatcaagaaa atgccaggaa
     9661 cagagaaaca caaagcaaca aaccagtgac gataatcagg tacagagaca tccctttgta
     9721 tttgtgcaat gccggtactt tgtaaaccag ccacgccggg atcaggcacc ctaccatgcc
     9781 gaaaatcggg ctacagatag aggtgaagct caacaccggt gcgttcagta cgatggcgct
     9841 ccaggccagc aaaatggcga aaatcatgat gccgcgctga acaagatttt cgttaatctt
     9901 ctcggcaggc atcttgcgac gcaggatgtt cattacgatc ccttgcgttg cttcgcgaaa
     9961 gcctaaatag acgccaaaga atgcagtcat gacagcaaag atattgagaa tgacgctgac
    10021 cactttcacc cacgctgcgc cgtcaccgct aataaactgt gcggcaatcg ccagcgcgga
    10081 aatattctgc tcgtaggctt ttaccgcttc gtcatgtccc atcgccagcg tgaacgacac
    10141 ggcgtagaaa aagacggtga caaacaaaat gccaaacgcg atattcattg cccgcaatgc
    10201 tttatgccgc gccacttcaa ttgatttttc ccgcgagcga taagagatca ccatcgggct
    10261 taacgtctgg ataaacagaa tcgacgtcag ggtaaacggc agcgtaataa tggcgttttt
    10321 caccagcagc cccagcggcg gtagcgaacc gacgttatac agatgccaca ttcccaccat
    10381 cgacacgccc agcgcagcga ctaccagcag cttggtcagc accatgtcgg tcgaaatttt
    10441 gaataacaat ttctcgccgc gtgaagagat cgccaccaga atgcaaatca gtaccagacc
    10501 ataaaaggga ctgtctgaca gcaacccttc cgtcacgccg aaggtatgca gataggaagc
    10561 actatcgttg gtgatggcgg tggaatagac gaacatccaa atcaccagca ttacgaaata
    10621 gagcgcacct aacaggatgc cccagttttt acctaaataa ccgctaatga cactcgggta
    10681 atctttacat tctggtgatt ctgccagcgt attaataaac aaccgctgaa acagatacat
    10741 tgccgggtaa ccaatcaccg atgagagcaa aaatacccac aatcccatca aaccgacctg
    10801 caccgggaga aaaacaatcc ccgcgccaat cgccatcccg atactcataa tcacccagcc
    10861 ggtgtcagta ctgtcgaatt tgatcgcttc tcgccactcg ctctcactca ttccagcacg
    10921 acccgccggg gtcgaagcgt ctgcaatgac gcctttattc gatgcaattt ccataatttc
    10981 tcgctcaata ttttgtaggg cttattattt ttttccgagc cgcatcaagg cgatatgcgg
    11041 tctacgtttt tgcaggcgag ttattagaat aaagaaatga tacgcgcgag gtcagagaaa
    11101 atcttcacaa tcaagacttt aaaataacgg agatggataa gaattttcta ctaaattaat
    11161 cacaagagaa tattcattct ctatttgcgg cagatcacaa aaaaaaggtg cacatttgtg
    11221 cacccaagga tgaaagctga cagcaatgtc agccgcagac cactttaatg gccagtcctc
    11281 cgcgtgatgt ttcgcggtat ttatcgttca tatctttgcc ggtttcatac atcgtctcga
    11341 tcactttatc gagtgaaaca cgcggtgccg aggtgcggcg catcgccatc cgcgcggcgt
    11401 ttactgcttt cacggcatta atggcattac gttcaatgca cgggatttgt acctgtccgg
    11461 caaccggatc gcaggtcagc ccaaggttat gctccatcgc gatttccgcc gcattgcata
    11521 cctgcgccgg actgccgccc agtagttcag ttaaccctgc cgctgccatt gaacaggcca
    11581 cgccaatctc cccctgacag ccgacctccg cgccagagat ggaggcgttc attttataca
    11641 gcgcgccaat agcccccgct gccagaaaat agcgggcaat tgaccgctcg tttaccggac
    11701 gacggaactt atcgtaataa gccagcactg ccggaataat gccgcacgca ccgttagtcg
    11761 gtgccgttac cacgcgcccg ccagctgcgt tttcttcact aaccgccagc gcgtacatgt
    11821 tgatccagtc gatgacattc atcggatcgt tagagatgtt atcgctagaa accagctgac
    11881 gacgcagcgc tacggcacga cacggcacat tgagcggacc aggcagcacg ccttcagtgt
    11941 tcatcccgcg ttcaataccg tcatgcatca cttgccagat acgggcaaac cctgcgtcaa
    12001 tttccgcttt gctgcgcagc gccagctcgt tgtgcatcat caggccagat atagacaggc
    12061 cgttgtaatc acacattttc agcagttcac ctgctgagtg gaaatcgtaa ggtacggacg
    12121 tttcgacatc gtgcgacagg ccgaagtgtt cttcttcgac aataaacccg ccgccgacag
    12181 agtaataggt tttacttaat agcgcttcct gtcccttcca ggcagtgatc cgcattccgt
    12241 tctcatggcg aggcaacatt tcgggatgga agataatgtt ctttgctaca ggaaaatcaa
    12301 caatatgcgc accagatgcc actggcagcc gcccgctgcg cgttactaac tctataaatg
    12361 cagggatctc atcaatgaca acatcctgcg gactgtttcc tgccagtccc atgataatgg
    12421 cgacatccgt ggcatggcct ttgcccgtca gtgacaacga cccgtacaga tcgaccacaa
    12481 tatggctcgt cgcggttaat aagccgctac tttccagccg atcaataaaa ctttttccgg
    12541 cattcattgg ccccacggta tgcgaactgg agggaccaat cccaattttg aaaatatcga
    12601 atgcactaat catatccaca ccctcggatt gccgttcagt gaagtggagc ggaacgacct
    12661 tgcgaccgtc ccgctcacga ggctttacgc actacgtact gcgatagctt caatttccag
    12721 cttcacatct ttcggcaaac gcgcaacctg cacacagctc cgggtcgggt aggtcgcctg
    12781 atgctcatcg aagaactgct tatagacttc attgatggtg gcaaaatcat tcagatcggt
    12841 gatgaacacg gtcatcttga tgatatcgcc cacgctcagc ccggcagcaa ccacgatcgc
    12901 tttgacgttt tcgaggctta aacgcgcctg atcttgcaca tcagccggga tctcaccggt
    12961 ctgcgggcaa accggaattt gcccggaggt gaagaccatg ctgcctaaat caacgccctg
    13021 aacataaggg ccgattgcgc ctggggcacg ttgcgtttcg ataatctttt tcatacatcc
    13081 tccggcgtca gagcgcctgg gtaaaagtac gtgaaataac atcctgttgc tgttcacggg
    13141 tcagtgcgtt gaagcgcacg gcgtagccag agacacggat tgtcaggtta ggatattttt
    13201 ccgggtgctc gatggcatcc agcagcattt cccgattcat tacgttgacg ttgaggtgtt
    13261 gaccgccttc gacatccgct tcgtggtgga aatacccatc cagcaggccg acaaggttgg
    13321 ttttacgtac tggatcttct ttgcccagcg ccgcaggaac gattgagaag gtgtacgaga
    13381 tcccatcttt ggcgtaggtg aacggcagtt tcgccaccga cgtcaatgag gccacggcac
    13441 ctttgcggtc acgaccatgc atcgggttag cgcccggcgc gaacggtgtt ccggcgcgac
    13501 gaccgtccgg cgtattaccg gttttctggc cgtacaccac gttagaagtg atagtcagaa
    13561 tcgactgggt agggacggcg ttgcgatagg ttggcagcgc tttaattttc ttcataaagc
    13621 gttcaaccaa gtcgcaggca atgctgtcta cgcgctcgtc gttgttgccg tactgcggat
    13681 attcaccgtc gatttcaaag tccaccgcca ggccgttttc gtcacggatt ggtttcacgc
    13741 gggcatattt gatggcagac agggagtccg tcgccaccga caggcccgcg atgccgcatg
    13801 ccatagtgcg atagacatca cgatcgtgca gcgccatcag cgaagcttcg tagctgtact
    13861 tgtcgtgcat gtagtgaatg atattcagcg cgctgatgta ctgcaccgcc agccagtcca
    13921 tgaagtgatc gaggctgtcc atcactttgt cgtaatccag cacgtcgtcc atcagcggtg
    13981 ctgttttcgg cccgacctga atcttcagct tctcgtccac cccgccgtta attgcgtaga
    14041 gcagcgtttt cgccaggtta gcgcgtgcac caaagaactg catttgctta ccaatcacca
    14101 ttgggctgac gcagcaggca atcgcgtaat cgtcgctgtt gaagtcagta cgcatcagat
    14161 cgtcattttc atactgcaag gaagaggtga cgatcgacac ctgcgcggca tattttttga
    14221 aggcaatcgg taattcttcc gaccaaagaa tggtcaggtt aggttccggt gccggcccca
    14281 tagtgtgcag ggtgtgcaaa tagcggaagg agtttttggt caccagcgta cgaccgtcca
    14341 gccccatccc gccgatcact tccgtcgccc agattgggtc gccggagaac agcgaatcaa
    14401 attccggtgt acgcaggaag cgtaccatac ggatcttcat gatgaagtga tcgatcagtt
    14461 cctgtgcctg ctgctcattg aggacgccag ctttaaagtc gcgctcaatg tagatatcca
    14521 ggaacgatgc cgtgcgcccc agcgacatcg cgccgccatt ttgcgatttc actgccgcca
    14581 gataagcgaa gtagagccac tgcaccgctt cctgtgcatt ctgcgccggg cgagagatat
    14641 caaagccata tttcgccgcc atttcctgaa tctgcaacag cgcatgacga tgctctgcca
    14701 gctcctcacg cagacggatg gtggcttcca gatcctcgcc tttttccaga cgagactgga
    14761 gatcggcaaa ttgcagttcg cgttcacgca ccagataact gatgccatac agcgctacgc
    14821 ggcgatagtc accgataatg cgcccacgac catagccatc tggtaaaccg gtcagcacgc
    14881 cagatttacg gcagcgcagc atatccggtg agtaaacatc aaatacgccc tggttatggg
    14941 ttttacgcag atcggtaaac agatattcaa attcactgtc catttcacgg ccataggcgt
    15001 ggaatgaact tttaatcata ttaatgccac cgaacgggtg tagcgcacgt ttcaacggcg
    15061 catccgtttg caggccaaca attttttcca gcggctggtt aatatatccc gcatcatgag
    15121 cggtaattgt ggtggcaata ttggtatcga aatcaaccgg cgcgtgggtt gcgttttcga
    15181 tacggatgcc ttccattacc ttttcccaca attccgtggt ggcaggcgtc gcttcggcga
    15241 gaaaagattc atcgccttca tacggtgtat agttatgttg aataaaatcg cggacattaa
    15301 tttcgctttt ccagtccgta cctttaaagc caagccatgc gtcagcgtac agcttatcgc
    15361 tggtatcaat atctaccttc atgaaaaata atctctctac aatacttcaa ctaaattatg
    15421 caaattctgc gggcgcgtta actttaccta aatgaatggc atccaaagca atcatttttt
    15481 cttcgttagt cggaataacg acgcaaatga catgcgcatt ttcactggaa acaattcgct
    15541 cgccaaaaga gttagaacga ttattcattt ctgtatcaat ctctacgcct aatacagcca
    15601 aatgttccat gaccagacga cgaattaaac ttgaattctc tcctattccg ccggtgaata
    15661 taattccatc cagccgacgt aatgaagctg catgtccggc aatatgacgg gcaattcggt
    15721 gaacaaaggt tttaattgcc agttgcgcgc gttcgtgacc ttcatgccag gctttttcca
    15781 gaacacgtaa atccgaagaa agaccggaaa tacctaataa tcccgactct ttattcacta
    15841 cgcgttccag gtcacccagg ctctggttgg tttggctggc gacccaggac atcgcaccaa
    15901 agtcgacatc gccactgcgg gtacccatca tcaagccttc cagcggcgtc attcccattg
    15961 aggtgtcaac actctgaccg ttgcgaactg cgcagattga cgcgccattg ccaagatgcg
    16021 ccacaaccag gccggagtca tcttccgcca gattcagcag cgaatgtgcg cgctgggaaa
    16081 catagcggtg cgacgtgccg tggaaaccat aacggcgtac acctaactct tcataatatt
    16141 tccacggcag gccgtataaa taagcttccg gagccatcgt ctggtggaaa ctggtatcaa
    16201 ataccgccac ctgagttacg cccggaaata attgctgcgc cgattcaata ccacttaaat
    16261 ttgcgtaatt atgcaggggt gccagtggag aaacgcgacg gatattatca atgacttcat
    16321 cggtaataat ggcggactcg gtaaaaatac tgccgccgtg agcgatgcgg tggccaatta
    16381 aggccacgct gtcatttaaa ttccgttttt ccagttcaaa tgcaattgcc ttcaatgcac
    16441 cttcgtagct gtggtgagcc agcggtgctg gctctccccc atttacggat aagaatgcat
    16501 tttccgagtt aataccgtcg gcaatacctg acattaatac ttcacaatcg ctggcatcga
    16561 gtacggaaaa cttaatcgaa gacgaaccac agttaataac caaaacaacc ggaaattcat
    16621 tcatctcttt tctcatcctg agttacggat taaaacagtt tgtatacgat gttcaggatg
    16681 gtcagcagac caatcacggt aacaaacacg ttatccagac gaccacggta tttcgccaga
    16741 gacggcgctt tacggatagc atacatcggc aacaggcaca gcagggatgc gataatcggt
    16801 gcgcccatgg cttcaatcag gtcgaggatg ttcgggttgg cgtaggcaac aacccaggtg
    16861 gagcccatga tgaagatcat gctgagagta ttcagtttac ccagcgacac tttggttttg
    16921 tcacctttat aaccgaactt cagaatcaga ccattcaagc cttccagcgt ccccagatag
    16981 tgaccgaaga aagatttgaa gatagccacg agtgcgatga tggaagccgc atattccagt
    17041 gtaatcgcga acgttgtttt cgtaccggtc atggacgcaa agtggttagc cagataagaa
    17101 agcactggaa tattctgcgc tttggcttcc gccatgttgg ccggagacag agtaaacagg
    17161 cagctaaagg caaagaacat caccactgca accatcagca tgctggcacg agaaatgatt
    17221 tgggaacatt tacgttcggt gaagtcgcga ccgaagtctt tctcatactc ttcacgttta
    17281 gaaaccacga aggaagagac gattggcgag aagttaaagg agaaaaccat gatggaaatc
    17341 cccagccaga cagtgatcag gataccgtca tgaccggtta acgacagcga accgaggtca
    17401 acctggtcga taactgcaga gttccagtaa gggatcagcg acagagaaat cagcaccagg
    17461 ctggcgataa acggccatac caggtagctc atcactttaa ccatcagatc cttaccaaac
    17521 cagatgacga aagccatcag cagcaacagg aacagcgcca caaagccgcg attcagcggt
    17581 gcaaatccga gctggttttc ccagaacgtc ataaaggtat tggtaatagt aacgccataa
    17641 atccacagca gtgggcaaat cgcgaagaag tacaggaacg tgataaccac gccgccagtt
    17701 ttaccaaaat gctcttccac cgtttccgta atgttgccgg aagggttaga gccagaaaga
    17761 cacagacgcg ccagcgcccg gtggcaataa aatgcgatgg ggtatgccaa taccaacatc
    17821 agaagaatcg ggatcagtcc gccaaaacct gcgcggatag ggaagaacag caccccggcg
    17881 ccgattgccg taccaaacaa gcctaacgtc catgtggtat ctgatttacg ccaggacgat
    17941 tgttttgtct ggctggatac aatgctatct gaagtactca tatcctatcc tcaacgaatt
    18001 aattaagcgt caacgaaacc ggtgatttga gagacgcgag aaagatcgat attgccgccg
    18061 gaaataatac tgacggtttt tctattttga atatattggt ctaatttacc gcttaataat
    18121 gcagcacatg ccagagcgcc tgcgccttcg gtgacgactt tattgcgctg aattaaggca
    18181 atcatactgt ttctgatttc gtcttcgctg accagcacga tgtcatcgac taattcacga
    18241 acgatttcgt aagttaaatt acccgggcgg gagacatcac aaccatccgc cagggtgccg
    18301 gtagttcggt gcgtggttat ttctccggag tggaaagaag ccgccatgcc gtgaacgttt
    18361 tctgactgta cgccaataac acgaatggtc gggttaatag atttaattgc caccgcaata
    18421 ccagcaatta aaccaccacc accaattggc acaatcacgt tatcgacatc atagagatct
    18481 tccataattt ccagaccaat cgttccctgg ccagcaatca ctttcggatc atcgtaaggt
    18541 gggataaaaa tacgaccttc catttcgaca atttcgctca ctttagcgat agtgtcgttg
    18601 aagttatcac catgcagaac gacttctgcg gagtagtcgc acgttgccgc tactttggat
    18661 tttggcgcac cttttggcat caccacttta ccgtcgatac ccagcatcgc gcaggagagc
    18721 gaaacccctt gcgcatggtt gcccgcagaa caggccacca cgcctttgcg tttttccgca
    18781 tcggtcagtg aacttaattt attaaatgcg ccacgaattt taaatgaacc cgtacgctgc
    18841 atattttcaa acttcaggaa tatttcacct ttgcaacgtt cactaaaata gttggagcga
    18901 ggcatgcctg ttttataaat tcgcccagcc agtcgttgtt tcgcttcaat aatatcatca
    18961 atagcaaccg gcagatcgta tgtaatatgc attataaaac ctcttcgcct gaattaaata
    19021 tgtaggtaac cgtaaccgac acctgcaaaa cagacaggtg gattatttat gtttaaataa
    19081 aaacaaacaa ctaaccaact tctattaatt gccttcgtct acacccatta taagatgaat
    19141 actctttggc taattccacc aaaaccgatg ctgctttttt aatacgataa tttttcgacc
    19201 ataccgcggc atattgtgcc acaggtaatg tttcttcaac cggaatagta ataaattgat
    19261 tagaaccaaa aggtgacgtc atatcacaag gaattacagt taagaaatca gcattgagaa
    19321 caagattata aattgtcacg actgagtcgg ttttaacgat gttttcaata ctgatgccat
    19381 ttctttgtaa cgtagtaagc agttcgctgt agtaccccat attagtttgt ggcaacaccc
    19441 actgttcgtt cttcaacgac tccagcgtgg tggtgccggt gcatgttcgg gacttactgg
    19501 ctaccagcac aaactcggac tcgaacagcg gctcaacatg taaatcctga agcttcattt
    19561 ctgcacttaa cgtaccaatc gcaaaatcca gtcgaccgtc gcggattgcc ggtaagaacg
    19621 aagacagttg cgcttcatac atagaaacct gcgctttcgg gaacacctct ttgaacttgt
    19681 tgatcatccc tgacataaaa gtaaaaccaa tcaatgaagg aaaaccaaat gagacttcca
    19741 ccaccgcttc agaagacata ccgcttatct cattaaccat atttttcatt tcacgggtaa
    19801 tggattcgga acgggagagt aacaattgac cggcaggtgt taatgttaca ccggtatttt
    19861 tccgcaccac taattccaca ccaaaataat cttcaatatc gttaatgatt ttactgacgg
    19921 ccggttgagt taaccctaat tcttttgcag ccgagccgat agagccactt ctaatgactt
    19981 cctgaaagac taccaggtgc tgcgttttcg gaagaagaat agtgctcata acgacctacg
    20041 ttaattacct cattgacggc atgaagtgta tcaaaatgaa ataaacagga tatgtgcgac
    20101 cactcacaaa ttaactttca ttactttcca
//
LOCUS       DAEBXN010000079        19702 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.079, whole genome shotgun sequence.
ACCESSION   DAEBXN010000079 DAEBXN010000000
VERSION     DAEBXN010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19702
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.079"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(151..1584)
                     /gene="hldE"
                     /locus_tag="KJL99_002830"
     CDS             complement(151..1584)
                     /gene="hldE"
                     /locus_tag="KJL99_002830"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HBD4437495.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVFYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(1632..4472)
                     /gene="glnE"
                     /locus_tag="KJL99_002831"
     CDS             complement(1632..4472)
                     /gene="glnE"
                     /locus_tag="KJL99_002831"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HBD4437496.1"
                     /translation="MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPLLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTMIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(4495..5796)
                     /gene="ygiF"
                     /locus_tag="KJL99_002832"
     CDS             complement(4495..5796)
                     /gene="ygiF"
                     /locus_tag="KJL99_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HBD4437497.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEI
                     WPNGELPADLASRVQSLFSTDFYREKWLVEIDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELVLAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            6038..6658
                     /locus_tag="KJL99_002833"
     CDS             6038..6658
                     /locus_tag="KJL99_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBD4437498.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            6722..7960
                     /gene="cca"
                     /locus_tag="KJL99_002834"
     CDS             6722..7960
                     /gene="cca"
                     /locus_tag="KJL99_002834"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HBD4437499.1"
                     /translation="MKIYLVGGAVRDALLGLSVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYTAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIRTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREGWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(8123..8944)
                     /gene="bacA"
                     /locus_tag="KJL99_002835"
     CDS             complement(8123..8944)
                     /gene="bacA"
                     /locus_tag="KJL99_002835"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBD4437500.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(9035..9403)
                     /gene="folB"
                     /locus_tag="KJL99_002836"
     CDS             complement(9035..9403)
                     /gene="folB"
                     /locus_tag="KJL99_002836"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HBD4437501.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            9508..10125
                     /gene="plsY"
                     /locus_tag="KJL99_002837"
     CDS             9508..10125
                     /gene="plsY"
                     /locus_tag="KJL99_002837"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HBD4437502.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPISWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(10138..11069)
                     /gene="ttdR"
                     /locus_tag="KJL99_002838"
                     /gene_synonym="dan"
                     /pseudo
     CDS             complement(10138..11069)
                     /gene="ttdR"
                     /locus_tag="KJL99_002838"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
     gene            11276..12119
                     /gene="ttdA"
                     /locus_tag="KJL99_002839"
                     /pseudo
     CDS             11276..12119
                     /gene="ttdA"
                     /locus_tag="KJL99_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
     gene            12116..12721
                     /gene="ttdB"
                     /locus_tag="KJL99_002840"
     CDS             12116..12721
                     /gene="ttdB"
                     /locus_tag="KJL99_002840"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HBD4437503.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            12769..14232
                     /gene="ttdT"
                     /locus_tag="KJL99_002841"
     CDS             12769..14232
                     /gene="ttdT"
                     /locus_tag="KJL99_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HBD4437504.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVTLIVVFYLLRY
                     FFASATAYTCALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(14275..15288)
                     /gene="tsaD"
                     /locus_tag="KJL99_002842"
     CDS             complement(14275..15288)
                     /gene="tsaD"
                     /locus_tag="KJL99_002842"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HBD4437505.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            15526..15741
                     /gene="rpsU"
                     /locus_tag="KJL99_002843"
     CDS             15526..15741
                     /gene="rpsU"
                     /locus_tag="KJL99_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HBD4437506.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            15852..17597
                     /gene="dnaG"
                     /locus_tag="KJL99_002844"
     CDS             15852..17597
                     /gene="dnaG"
                     /locus_tag="KJL99_002844"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBD4437507.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            17792..19633
                     /gene="rpoD"
                     /locus_tag="KJL99_002845"
     CDS             17792..19633
                     /gene="rpoD"
                     /locus_tag="KJL99_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HBD4437508.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         4682 a         5507 c         5034 g         4479 t
ORIGIN      
        1 gttgttactg ttaagtttcc cgacttacta acaactgatc agagggggga gaaatcctcc
       61 cttacccttg ttcctttact ttaggttgaa aaaacaacag cgtcaatagg cctgcctgct
      121 acgaagcgag atctgtgaac cgctttccgg ttagcctttt ttatcctgtt ggatcttctt
      181 gatgatgtta gtggtcgagc aaccgtcttc gaagttgagc accagcactt cgccgccgtt
      241 ggcccagact tctttactcc cggcgatctc ttctggttta tagtcgccgc ccttcaccag
      301 taaatctggc aggatcccgg cgatcaagcg ctgcggcgtg tcttcttcaa acgacaccac
      361 ccagtcgacc gcttccagtg cgcccagcac aatcatacgc tgttcgagcg ggttaacggg
      421 gcgagaatcc cctttcagcc gtttggtgga ggcatcgctg ttcacagcga caatcaagcg
      481 gtcacccagt ttgcgggcat ttgccagata gaagacgtgt ccggcgtgca ggatgtcaaa
      541 gacgccattg gtcatcacca ctttttcacc gcgtttacgc gctgccgcta cggccagctt
      601 cagttcctct tcggtcatca cgccaaagcc ggtatctgcg cgcccacgca ctgcattttc
      661 cagctcgatc ggcgaaaccg tggaggttcc cagtttgccg accaccacgc cagccgccgc
      721 attggcaaag aagcaggctt cttccagcga attacccgct gccagcgttg ccgccaggac
      781 gccaatcacc gtgtcgcccg caccggtaac gtcatacact tcctgcgcct gggttggcat
      841 atgcagcggc gctttacccg gttgcagcag cgacataccc tgttcggaac gggtcactaa
      901 cagagccgag agttcgtaat cggcaatcag tttcatgccg cgctcaacaa tctcttcttc
      961 ggtcttacat ttaccgacaa cagcttcaaa ttccgagaga ttcggtgtca gcagcgtagc
     1021 gccgcggtag cgctcaaaat cggtaccttt tggatcaatc agcaccggaa cgcccgcttt
     1081 acgcgccagt tggatcatct gctgtacgct tgccagcgca cctttggcgt agtcagaaag
     1141 caccagcgcg ccaatcgaac tcagcgcctg attaatccgc tcgtgcagcg gctgcggatc
     1201 aacaccttcg aaaccttctt caaaatccag acggatcagc tgttggttgc gggaaagtac
     1261 ccgtaatttg gtgatggtcg gatgcgtcgg tacagaaacg aagtcgcatt tgacgttgac
     1321 gtcggccaga gatttactca gcgcgcgcgc tgcatcgtca atgcccgtca acccgaccag
     1381 gcgtgcatta gcaccgagag aagcgatatt catcgccacg ttagccgcgc cgcccggacg
     1441 ttcttcgatg gtattcactt taaccacggg caccggcgct tccggcgaga tacgactggt
     1501 ggggccgtac cagtaacgat ccagcatcac atcaccaacc accatcactc ctgcacgttc
     1561 aaactctggc agcgttactt tcattcctgt ctcctgagag attcaaaatt tgcgcgcgat
     1621 aataccatac ttcattcttc caccagccac ttctgccagc ttgcccgcac cagatcacgc
     1681 tctgcggtga agcaatcctc cgacacatgg cccggcaatt cctgtaatgc cagatgatga
     1741 agttcatcgc gcagcgtcgt gtaagcacgg gtcagcgcca tcgcttcctg ctcttccata
     1801 atgtcgtttt gcgccagcag ctccagaata cgcacgttgt ctgaccagcg cgttaacttc
     1861 ggtttttcat gagcgtagcg caacaccaga tattgggtaa taaattcgat atcggtaatt
     1921 ccgccttcat cggctttgat atcaaagcga ttacgatgtt tattgccgag atgagcgcgc
     1981 attttctcgc gcatttcccg cacttccgtt tgcagagttt taccttctcg cggcagtgtc
     2041 ataatctcac gacgcaccgc gtcaaagtgc gcagtgagct gcggatcgcc gtacactaca
     2101 cgcgcacgca ccagcgcctg atgttcccac gtccaggcct cgtttttctg ataatcggca
     2161 aatgcttctg cggatgtcac cagcattccc gccgccccgg acggacgcag tcgagcatcc
     2221 acttcataca aaatgccgga agaggtacgc gtactgaaca gatgcataat gcgttgcgcc
     2281 agacgcagat aaaactgccg cccgtcgatt tcccgctcgc cgtcagtcat cgcatccatt
     2341 gggcaatcat ggaggaagat aaggtcaaga tcggaactgt agcctaactc ccagccgccc
     2401 agcttgccgt agccgaccac cgcaaaaccg cgcccttcac gttcgttgag gtgatttggc
     2461 ttaccgtagc gggccaccat ttgaccccac gcctgctgaa cgacggcatc tatcatagct
     2521 tctgccagcc aggttaagtg atcgctcact ttcattaccg gtagcgtacc ggcgatatcc
     2581 gctgccgcaa tgcgtaacag ctgcgcctgt ttgaactgac gcagcgcctc aagctgttgt
     2641 tcttcgtcat cttccggcac gcgcagcaaa tactggcgca gttcatcacg gtaggcatcg
     2701 gtcgccgtcg gctgataaag ggtatttgga tcgagcaatt catccagcaa taatggataa
     2761 cgcgctagct ggctggcaat catcggcgat gcggcacaca gagaaatcaa atgtttaagc
     2821 gccgcaggga attcactgag caattctaaa taggttgtgc gggtaacaat ccccaccagc
     2881 aaggcggtaa tgcgcgacag cgtgacagca gcgtcttcac gcgcacagac atcacttagc
     2941 agatgtggca tcagatggtc gagcacctga cgtcctcgcg gcccgatagt acgcttatcc
     3001 agctctttgc ggaaatcagc aatcatcgtt aggacctgtt tgcgatcatc ctcgctaaga
     3061 tgcgccagca ctggcgtagt gtcatcttcc tgcaacgcat cctgccacag ctcacgccac
     3121 tgttccgaca gcgactcttc ctgagtttca ctttcgtcgt cgccaatcaa ttcattaaac
     3181 acccggcgca cattggtcat atgtgcggtc agcgccccgg tcagttgcgg ccagtcagca
     3241 aagtccatcg cccacgccag ccgcgcacgg ttaagctcat cagaaggaag cgtttgggtt
     3301 tgttcgtcgt taatgctttg cagcaggttt tccagacgcc gcaggaacag atatgccact
     3361 cgcaattgtt cagcatcgtt ttcagaaagc agatgcagcg cggcaatggc gctgagcgtt
     3421 ggcagtaaag agcgcgactg cagcaacggt tcgcgcccac cgcgaatgag ctggaacacc
     3481 tgaacgataa attcaatttc gcgaatgccg cctgcgccga gtttgatatt gtcggtcaaa
     3541 ccacgtcgac gcacttcacg ggcaatcatc cctttcatgt tgcgcagcga ctgaatcacg
     3601 ctgaaatcga tgtaacgacg gaaaacaaac gggcgcagca tcgcacgcaa ctcgttagca
     3661 tagacgcctt cgctatcgcc cataatccgc gctttgacca tcgcgtaacg ctcccagtcg
     3721 cgtccctgct cctggtaata atcctccaac gcggcaaagc tcagcaccag cgggccactt
     3781 tcgccaaacg gacgcagtcg catatccacg cgatagacga agccatcctg cgttggttga
     3841 tccagcactt taatcagccg ctgccccatg cgggtaaaaa actgcgcgtt atccagttcc
     3901 cggcgtccgc cctgcgtaca accatgttcc ggccaggcaa aaatcagatc gatatcagag
     3961 gagaaattca gttccccgcc gcccagctta cccatgccta agatcagcag cggttgcgct
     4021 tcgccctgcg cattgcacgg cgttccccac tcgcggcagc aggcgtcata cagccagtca
     4081 cgcgccgcaa caatcagcgt ctccgccaga taactgagct gctgcaatat gctctcttca
     4141 gtaaccagtg ccagcgtttg cgcccatgcg atgcgcacca taatgcgccg ccggaatagc
     4201 cgcagctcgc gcattaaccc ggcttcgtcg ctcacattac tgagcgcctc ctgcaaccac
     4261 acgctgtaat gttgccattc gtcagcctgt ggcggttggc tttccagttc cgtcagccac
     4321 tctggatgcg caatcacgct gtcctgcaca aaatcactga aagtaagtac tgacttcgcc
     4381 tgtgcactaa gtgattcctc ggctaaaagc tctggcagcc gctcaacaac ggtctgccag
     4441 tactgctgta acggtgaaga gagcggcttc ataagcgatt ttatccttgc ctgattaacg
     4501 ttttccgctg tgcaaccaga acggttcctg attgtttgcc tcattacgga aatgttcaat
     4561 ttcaatgcgt tgcccggtcg caatagcgtg acgcagcccc tgccagttct ccagccaggc
     4621 ttgcgcgacg acaggatcat agtaacccgc cagcaacagt attgagtcaa tatcacgcat
     4681 caggcgtggc aactggtcac ggtagcgatc gcctaacggc tggcagaaaa cgcttttcag
     4741 ttcagcggca tggcgggaaa gatggatatc ggcaaagcgt ttgaaggagt cgctaatttt
     4801 gccctgcgct ttggcatcta aaaacggctg ccatgctttg cttaccagcc attcggtcaa
     4861 cgccagcttc gccattgccg tttcggtaga gtagaccgcc gtcacggcag aaaccgcaga
     4921 agcgatggtc gcttcgcatt gagtcagcaa atcacgtaag tgagtactcg ctttacgcgg
     4981 cacaatgcca ccgaacagca tcagtgtatg acggactagg ctaatggctg ccagcacctg
     5041 ctctttcgcc gcatcgttgc cgcgtaccca cagttcttca tgatactgcc attgcgctaa
     5101 caccagctcg agcgccgctt ccagcccctg ttccacatcg gcttttgccg caacatgcaa
     5161 aatggtggtc ggtttgattt cacgcgccgg attgccctgc gccagatgat agccacgcgc
     5221 cgctttgctc aggctgccct ggcgtaatcc ggtttgcgat accagttggt tcgccagttt
     5281 cagcaccgcg cgcgtgtcgc cgctaagcag ctccagttcc agctcgcaga taggctcagc
     5341 aaattcaccc gctttcactt ccccctggtc gagagcgatt tcaattcggc tgccatcgat
     5401 ctctactagc catttttcgc gataaaaatc ggtgctaaac agcgactgca cgcgggaagc
     5461 gagatcggca ggcaattcgc cgttcggcca gatttccgtc ggtaactgcg ccaggtcgag
     5521 cgtcggttcg ctcaacgcca cgttatattc cgggcgctgg tgtaagccgc ctgtcactct
     5581 tcctgcaact ttcatggtca tctcatagcg accgttttcg ccacgaatac gtaagcccat
     5641 atcgtgccta cgcagccagt tatccggcgt ttcgtagtaa atattcagca actgcacggg
     5701 gtcatggtgc tcgccgccca gcgtattgag atggtcacgc aacgcctcaa cggcactgtg
     5761 attaacaata aactttaatt cgatttcctg agccatggcc tttacttatc ggttatgtca
     5821 cataagtgac gatgaacggc gaacttaatg cgatcttttt tgtcagtaga tagtattttg
     5881 cgccaaattg ccatgcaacg ggcaatttga cgggcgtaaa agtttgaagc agtggcaaga
     5941 cctgacacag aggattccga ttgatgtcga atgctttgcg tagcgacact gatgccacta
     6001 ctatcgttcc actttccatg acaataacga cagcctgatg ccaaaattac gcctgatcgg
     6061 attaacttta ctcgcactta gcgcgactgc cgtctcacac gctgaagaaa cgcgctatgt
     6121 ttctgacgaa ctgaatacct gggtccgtag cggtccggga gatcattatc gcctcgtggg
     6181 cacggttaac gccggcgagg aagtgacctt attacaaact gacgccaaca ccaattatgc
     6241 ccaggtgaaa gacagctctg gccgtaccgc ctggatcccg ttgaaacaac ttagcactga
     6301 gccaagcctg cgctcccgtg tgccagatct ggaaaatcag gtcaaaaccc tgaccgataa
     6361 actcaccaat atcgataaca cctggaatca gcgcaccgca gaaatgcagc aaaaagtggc
     6421 gcagagcgac agcgtgatca acgggttaaa agaagaaaat cagaaactga aaaacgagct
     6481 gattgtcgcg cagaaaaagg tcgatgccgc cagcgtacag ctggatgaca aacagcgcac
     6541 catcatcatg caatggttta tgtatggcgg tggcgtgctg gggcttggct tgctgctcgg
     6601 tctggtactg ccgcacctga tcccaagccg caaacgcaaa gatcgctgga tgaactaaat
     6661 cgccttcttc gttgcactgt cttattatta ggaattgtgt atttgacgaa gggatgatgg
     6721 cgtgaagatt tatctggtcg gtggtgctgt tcgggatgca ttgttaggac tatcggtcaa
     6781 agacagagat tgggtggtgg tcggcagtac gccacaggag atgctcgacg cgggctacca
     6841 gcaggtaggc cgcgattttc ctgtgtttct gcatccgcaa acgcatgaag agtatgcgct
     6901 ggcacgtacc gaacggaaat ccggttccgg ttacaccggt tttacttgct ataccgcacc
     6961 ggatgtcacg ctggaagatg atcttaagcg tcgcgatctg accattaatg cgctggccca
     7021 ggacgataac ggcgagatta tcgacccgta caacggtctg ggcgatctgc aaaatcgtct
     7081 gttgcgccat gtttcccccg cttttggcga agatccgtta cgcgtactgc gcgtggcgcg
     7141 ttttgctgcg cgttatgccc acctcggttt tcgtattgcc gatgaaactc tgacgctgat
     7201 gcgcgagatg acccatgcgg gtgaactgga acacctgacg cctgaacggg tatggaaaga
     7261 gacggaaagc gccctcacca cccgcaatcc ccaggtgttc ttccaggtac tgcgcgattg
     7321 cggcgcactt cgcgttttat tcccggaaat tgacgcactg tttggcgttc cggcacctgc
     7381 caggtggcat ccggaaatcg acacgggtat tcatacctta atgacgctct caatggcggc
     7441 gatgctgagt ccgcaggttg atgtccgttt cgcaacttta tgccacgatc tcggtaaagg
     7501 actgacgccg ccagaacttt ggccgcgtca tcatgggcat ggcccggcgg gtgttaagtt
     7561 agtggaacaa ctatgccagc gtctgcgcgt gccaaatgaa attcgcgatt tagccagact
     7621 ggtggctgag tttcacgatc tcatccgcac cttcccaatg ctgaacccaa aaaccatcgt
     7681 caaattattt gattccatcg atgcctggcg taaaccgcag cgtgtcgagc aactggcgct
     7741 gaccagcgag gctgacgtgc gcggcagaac cggttttgaa tcagcggact acccgcaagg
     7801 ccgttggttg cgcgaaggct gggaagtggc gcagtcagtg ccgacaaaag cggtcgttga
     7861 agcgggattt aaaggcgtgg agattcgcga ggagctgacc cgacggcgga ttgcggcggt
     7921 agccagctgg aaggaacaac gttgcccaaa gcctgactga gtataaaact aaacgttagc
     7981 acgaaccgtc ccctcgcccc ttcggggaga gggttagggt gaggggaaca gaccagcacc
     8041 ggtgcgaaca atcaccccca ccccggccct ctccctacag agcgagggag aaaacccggc
     8101 ctgctcccga accttttctc aatcaaaaga acacgacata caccgcagcc gccacaataa
     8161 agcgataaat ggcgaacggg ataaacgaaa tgcgcttaat caattgcagg aaggttttaa
     8221 tcgctatcag cgccaccaca aaagcggtga taaacccaac ggcaaacatc gggatatcgc
     8281 cggttgtcag gaagccccag cttttgtaga gatcgagcgc cgttgcgccc atcatcatcg
     8341 gtaccgccag caggaacgag aactcggaag cagcgtaacg gctcacgccc atcagcatcc
     8401 cacctgaaat ggtcgccccg gaacgggaga aacccggcca cagcgccaga cactggaaac
     8461 agccaatcat aaacgcctga cgataggtca tatcatcaag acccggcgca cgcggctctt
     8521 tcggcttcag gcattcggcg gcaatcagca acaaaccgcc aacgaccagc gcatacatca
     8581 catttatcgg gttaaacaat gacttaatcg tgtcgtggaa taatagcccc agtaccaccg
     8641 ccggaatcat ccccagcaaa atgtggatca gcgttaaacg acctttgctt tcaccttcgt
     8701 gctgcaacgg gcggccaaag tggatgccaa tcaggccaaa cagacgccgc cagaacatca
     8761 ctactaccgc cagaattgat cctaactgga tcacaacttc aaaggttttc gccgtgtcgc
     8821 cctcaaaccc caacaagtga ccgacaataa tcatatggcc cgtgctggat accggcagaa
     8881 attctgtcaa tccttcgacc acacccaata ttgccgctat cagcagcgag tgcatatcgc
     8941 tcatcaataa acccctaaat tattaaaatg taccgcttgt ccgaactact gcagtatgac
     9001 caggttataa ccgtttggtt taacagctgt aaaattaatt attttctttc agattattgc
     9061 cacgctcaat gattacgcca acattcgccg cccgcgccac tgcgcctggc ttgctgagtt
     9121 tgatacgcac ccacggcgag ttgaagcgtg ctaacagcag ctccgccacc tcttcagcca
     9181 cgcgttccac cagcgcaaaa cgcgccccct cgacgtggct gaccaccgtt tctgcaatgt
     9241 cagcgtaact gaggcaatcc gccacatcat cacttttcgc cgctttacgg ttatcccatg
     9301 ccatttcgat atcgaacact aacttctgtt cgatggtctg ttcccagtcg taaacaccaa
     9361 tagtggtgat taccgaaagt tgctctataa atacaatatc catcacgtcc tgcctgcttt
     9421 ttggctaacc ggataccact tccggcgaaa tgtgcgtatt atccacagat tcatcgttga
     9481 acacgaattt tcaaaacgga acagcttatg agtgcaatcg cgcctggaat gatcctcatc
     9541 gcgtacctct gcggctccat ttccagtgcc attctggttt gccgcttgtg tgggctgccc
     9601 gatccgcgaa ccagcggctc cggcaatcca ggcgcaacca atgtgttacg tatcggtggc
     9661 aagggagcag ccgtagcagt actgattttc gacgttctga aaggaatgtt gcctgtctgg
     9721 ggcgcgtatg aattaggtgt cagccccttc tggctaggct taattgccat cgccgcctgt
     9781 cttggacaca tctggcccgt tttcttcggt tttaaaggag gaaaaggcgt tgctaccgct
     9841 tttggagcaa tcgcaccaat tagctgggat ctcaccggag taatggcggg aacctggtta
     9901 ctgaccgtgc tattgagcgg atactcgtcg ctgggagcga ttgtcagtgc actgattgct
     9961 ccgttttatg tctggtggtt taagccacaa ttcaccttcc cggtttcgat gctctcttgc
    10021 ctgatcctgc tgcgtcatca tgacaacatc cagcgtctgt ggcgtcgtca ggagacaaaa
    10081 atctggacga aattcaaaag aaagcgcgaa aaggatcccg agtgatttct ggtggatcta
    10141 catgacctga tagccttcat cgggcttgcc cagccgttgc tggcaccatg ccgccagaaa
    10201 ttccgcgcag acacgaattt catgctgcga tagagcagct cccggtaaac agcccagata
    10261 ttggcgcttt gtgcatactc cggcaatact tgcaccagtt tgccactctc cagaaacggc
    10321 aacacatccc actcggaacg cagcataatc cctttgccct ccagcgccca ttgcaacact
    10381 atctcgccgc tattggagga aagatgcccg ctaaccttca ccgatttttt ctcctgaccg
    10441 ttccccaact cccatattcc gtgggtcata tcgcgctctt tggtcaccag acagtcatga
    10501 cgacttaatt cctgtaatga ttgtggctgc ggatattttt gcagatactc aggtgctgcg
    10561 cataatattc ttttattttt cgttaacaga tgcgcaatat aataatcagg aatttcgtca
    10621 ttaatacgaa tatccagatc aatattatcc tgcactaaat caatttgccg atcgaacaat
    10681 tcaaaatgca cctgtaactc aggataattg cgcataagtt cggtaatggc tggcgcaata
    10741 tggctgcgcc caaaaccaaa actgcaacca atacgaatca tcccctccgg gcgcgttttg
    10801 atttgcgtga cgtcatcgac cagtcgctga tattgggtga ggatttccag cgcatgttcg
    10861 taacaacgtt ggccactttc agtcagcgcc acgccgcgag cggagcggtt cagcagcgtt
    10921 gttgccagcg tattttcaag gatttggatc cgcttggtga caaatgcagg cgtttgccca
    10981 agcgtcgccg ctgcagcact aaaactcccg ctgtggacga tttctaccag tacctgcaag
    11041 tctttggcta aaggccagct attcagcatc gttaattatc cgcagttgtg ataagcgcag
    11101 tgtatttcgc aaaacattgc cattccctgc tctggcgcaa tattcattct tattttcgcg
    11161 ttgattcacg aactgttaat tacatattaa cggtggcaaa aaatataaaa ccacattttt
    11221 agtgttagtt tgtggcggtg aatttttcca gacaaataca aaaactggag ttgccatgat
    11281 gagcgaaagt aataagcaac aggcagtgaa taagttgaca gagattgtcg ctaactttac
    11341 cgccatgatt tctacccgaa tgcctgatga cgtggtggat aaactaaaac agctaaagga
    11401 tgccgaaacg tcgtcgatgg ggaaaattat ctaccatacg atgttcgaca acatgcaaaa
    11461 agcgattgac ctgaatcgtc ctgcctgtca ggacaccggg gagattatgt tcttcgttaa
    11521 agtcggttcc cgcttcccac tgcttggcga gctgcaaagc atactcaaac aagccgtgga
    11581 agaggcaacc gtcaaagcgc cactacgtca caatgcggta gaaatttttg acgaagtaaa
    11641 caccggcaaa aataccggta gcggcgtacc gtgggtcacc tgggacatca tccccgacaa
    11701 tgacgatgcg gaaatcgaag tttacatggc aggcggcggc tgcacgctac ctggccgctc
    11761 gaaagtgtta atgccgtcag aaggctacga aggcgtggtg aaattcgtct tcgaaaatat
    11821 ctccaccctc gccgtaaacg cctgtccacc ggtactggtg ggcgtgggca tcgccacctc
    11881 ggtggaaacc gccgccgtac tctcgcgtaa agccattttg cgcccgattg gcagccgcca
    11941 tcccaatcca aaagcggcag aactggagct acgcctggaa gaaggactca accgtctggg
    12001 gattggtcca caagggctga ccggcaacag ttcagtgatg ggcgcatcgt cgcggcacgc
    12061 tgctggttca tgccgatctc acctttgaaa atctgtctca cacccggagc gcgttatgaa
    12121 aaagatcctg acaaccccga tcaaagctga agatctgcaa gatattcgcg tcggcgatgt
    12181 gatctacctg accggtacgc tggtgacctg ccgcgacgtt tgtcaccgcc gtttgatcga
    12241 actgaaacgt ccgatccctt acgatctcaa cggcaaagcg attttccacg ctggccccat
    12301 cgtgcgcaaa aacggcgaca aatgggagat ggtctccgtc ggcccgacaa ccagtatgcg
    12361 tatggaaagt tttgaacgtg aatttattga gcagaccggc gtaaaactgg tggttggcaa
    12421 aggtggtatg gggccgctga ccgaagaagg ctgccagaaa ttcaaggcgt tacatgtgat
    12481 tttcccagca ggctgcgcgg tgctggcggc aacccaggta gaagagattg aagaagtgca
    12541 ctggacagaa ctcgggatgc cggagtcact gtgggtctgc cgggtcaaag agttcggccc
    12601 gctgattgtc tctattgata cccacggcaa caacctgata gccgaaaaca aaaagctgtt
    12661 cgccgaacgc cgcgatccca tcgtggaaga gatctgcgag cacgtccatt acatcaaata
    12721 accctcccgg agaggctcac ccctctcctt tttcgcaggc ataacacgat gaaaccttcc
    12781 actgaatggt ggcgatatct tgcgccgctg gcggtcatcg ccattattgc tctacttccg
    12841 gttcccgcag ggctggagaa ccatacctgg ctctactttg ccgtttttac tggcgtgatc
    12901 gttggactga tcctcgaacc cgtgccgggt gccgtggtgg cgatggtggg tatctccatc
    12961 atcgccatac tctctccctg gctgctgttc agccccgaac agctcgccca gccaggcttt
    13021 aagttcaccg ccaaatccct ctcctgggca gtttctggtt tttctaattc ggttatctgg
    13081 ctcattttcg ccgcctttat gtttggcaca ggctatgaaa aaaccgggct tggacgccgc
    13141 attgcgctga ttctggtgaa aaagatgggg catcgcacgc tgtttctcgg ctatgcggtg
    13201 atgttctccg agctgatcct ggcacctgta acaccgtcca actcggcgcg tggtgcgggt
    13261 attatctatc ccatcatccg taacctgcca ccgctctatc aatcacaacc aaacgacagc
    13321 agttcgcgca gcattggctc gtacatcatg tggatgggga ttgttgccga ctgcgtgacc
    13381 agcgccattt tcctgacggc gatggcacct aacttgctgt taattggact gatgaaaagc
    13441 gcatctcacg ccacgctgag ttggggcgac tggttcctcg ggatgttgcc gctcagcatt
    13501 ttactggttc tgctggttcc ctggctggct tacgtgctgt acccgccggt actgaagtct
    13561 ggtgatcagg tgccgcgctg ggcagagacg gaactgcagg caatgggccc gctctgttcg
    13621 cgtgaaaaac ggatgctggg gctgatggta ggcgcgctgg tgctgtggat tttcggcggt
    13681 gattatatcg atgccgcgat ggtcggttac agcgtggtgg cgctgatgct gcttctgcgc
    13741 attatcagtt gggacgacat tgtcagtaat aaagcggcat ggaacgtttt cttctggctg
    13801 gcctcgctta tcaccctcgc gaccggactc aacaataccg gttttattag ctggtttggc
    13861 aaactgttag caggcagctt aagtggttat tcgccgacga tggtgatggt gacgttgatt
    13921 gtggtgtttt atctactgcg ctactttttc gccagcgcca cggcgtatac ctgcgccctc
    13981 gcaccgatga tgatcgccgc cgcgctggcg atgccggaaa tcccgctgcc ggtgttctgc
    14041 ctgatggttg gcgcggcaat tggtctgggg agcattctta caccatacgc caccggaccc
    14101 agtccgattt actacggtag tggttatctg ccaacggcgg attactggcg actgggggcg
    14161 atttttgggc tgatattcct cgtgttgctg gtgattaccg gcttactgtg gatgcccgtg
    14221 gtgttgcttt aagtgaggac ggtgccggaa attgctctcc ggcacccaat agttttacgc
    14281 agccggtaac tccgccagcg gccagcgcgg acgcacgcta acgccgagat ccgccgtcgc
    14341 gcctgcttta aaccgcacca ttccggcata ggcgatcatc gcgccgttat cagtacaaaa
    14401 ttccggacgc gcgtagaaca cttcgccgcg gcgttttttc atcatttcag ccagcttcgc
    14461 ccgtaacgtg cggttagcac tcacgccgcc cgccatgacc agtcgcttaa agcccgtctg
    14521 atccaacgct cgcttacact taatcatcaa cgtatcgacc accgcatctt caaaggcgcg
    14581 ggcgatatca gcacgcgtct ggtcgtcggt gccgttgtca cgaatcgtgt tcgccgcaaa
    14641 ggttttcaga ccagaaaagc tgaaatccag ccccggacgg tcggtcatcg gacgcgggaa
    14701 aacaaagcgc ccggcagtac cctgagccgc cattttcgac agtaacggtc cgccaggata
    14761 atccagcccc agcagcttcg cggttttatc aaacgcttca ccggcggcat catcgataga
    14821 ctcgccgagc agctcgtact gaccaatgcc agtcacgctg attaactgcg tatgaccgcc
    14881 ggacaccagc agcgcgacaa acggaaattc cggcgggtta tcttccagca tcggcgctaa
    14941 cagatgccct tccatatggt gtaccgggat tgccggaacg ttccaggcaa acgccagaga
    15001 acgccccacg gttgcgccaa ccagcagtgc gccgactaat ccagggcctg cggtataggc
    15061 cacagcatca atgtcttttg ccgttaaacc agactccttt agcgccgcct ggatcaacgg
    15121 tacggtttta cgcacatgat cgcgggaggc cagttcaggc acgacgccgc cgtagtcagc
    15181 gtgcaatttc acctgactat acaattggtt ggctaacaaa cctttttcat cgtcgtaaat
    15241 ggcgatgccg gtttcatcgc aggaagtttc aatacccagt acacgcatga cttgttttac
    15301 ctcgctttat taccgcgcag tgtaggacca atgcgggttg atgtaaaact ttgttcgccc
    15361 ctggagaaag cctcgtgtat actcctcacc cttataaaag tccctttcaa aaaaggccgc
    15421 ggtgctttac aaagcagcag caattgcagt aaaattccgc accattttga aataagctgg
    15481 cgttgatgcc agcggcaaac cgaattaatc aaaggtgaga ggcacatgcc ggtaattaaa
    15541 gtacgtgaaa acgagccgtt cgacgtagct ctgcgtcgct tcaagcgttc ctgcgaaaaa
    15601 gcaggtgttc tggcggaagt tcgtcgtcgt gagttctatg aaaaaccgac taccgaacgt
    15661 aagcgcgcta aagcttctgc agtgaaacgt cacgcgaaga aactggctcg cgaaaacgca
    15721 cgccgcactc gtctgtacta attccccgag agcgttgctc tccgatcaga ccgagttgta
    15781 gttgtaaggc cgtgcttccg aaaggaatgc gcggcttatt ttcgtttatg aattgctaaa
    15841 aatcggggcc tatggctgga cgaatcccac gcgtattcat taatgatctg ctggcacgca
    15901 ctgacatcgt cgatctgatc gatgcccgtg tgaagctgaa aaagcagggc aagaatttcc
    15961 acgcgtgttg tccattccac aacgagaaaa ccccgtcatt caccgttaac ggtgagaaac
    16021 agttttacca ctgctttgga tgtggcgcgc acggcaacgc gatcgacttc ctgatgaact
    16081 acgacaagct cgagttcgtc gaaacggtcg aagagctggc agcaatgcac aatcttgaag
    16141 tgccatttga agcaggcagc ggccccagcc agatcgagcg ccatcagagg caaacgcttt
    16201 atcagttgat ggacggtctg aatacgtttt accaacaatc tttacaacaa cctgttgcca
    16261 cgtctgcgcg ccagtatctg gaaaaacgcg gattaagcca cgaggttatc gctcgctttg
    16321 cgattggttt tgcgcccccc ggctgggaca acgtcctgaa gcggtttggc ggcaatccag
    16381 aaaatcgcca gtcattgatt gatgcgggga tgttggtcac taacgatcag ggacgcagtt
    16441 acgatcgttt ccgcgaacgg gtgatgttcc ccattcgcga taaacgcggt cgggtgattg
    16501 gttttggcgg gcgcgtgctg ggcaacgata cccccaaata cctgaactcg ccggaaacag
    16561 acattttcca taaaggccgc cagctttacg gtctttatga agcgcagcag gataacgctg
    16621 aacccaatcg tctgcttgtg gtcgaaggct atatggacgt ggtggcgctg gcgcaatacg
    16681 gcattaatta cgccgttgcg tcgttaggta cgtcaaccac cgccgatcac atacaactgt
    16741 tgttccgcgc gaccaacaat gtcatttgct gttatgacgg cgaccgtgca ggccgcgatg
    16801 ccgcatggcg agcgctggaa acggcgctgc cttacatgac agacggccgt cagctacgct
    16861 ttatgttttt gccggatggc gaagaccctg acacgctggt acgaaaagaa ggtaaagaag
    16921 cgtttgaagc gcggatggag caggcgatgc cactctccgc atttctgttt aacagtctga
    16981 tgccgcaagt tgatctgagt acccctgacg ggcgcgcacg tttgagtacg ctggcactgc
    17041 cattgatatc gcaagtgccg ggcgaaacgc tgcgaatata tcttcgtcag gaattaggca
    17101 acaaattagg catacttgat gacagccagc ttgaacgatt aatgccaaaa gcggcagaga
    17161 gcggcgtttc tcgccctgtt ccgcagctaa aacgcacgac catgcgtata cttatagggt
    17221 tgctggtgca aaatccagaa ttagcgacgt tggtcccgcc gcttgagaat ctggatgaaa
    17281 ataagctccc tggacttggc ttattcagag aactggtcaa cacttgtctc tcccagccag
    17341 gtctgaccac cgggcaactt ttagagcact atcgtggtac aaataatgct gccacccttg
    17401 aaaaactgtc gatgtgggac gatatagcag ataagaatat tgctgagcaa accttcaccg
    17461 actcactcaa ccatatgttt gattcgctgc ttgaactgcg ccaggaagag ttaatcgctc
    17521 gtgagcgcac gcatggttta agcaacgaag aacgcctgga gctctggaca ttaaaccagg
    17581 agctggcgaa aaagtgattt aacggcttaa gtgccgaaga gcgatcggga agcccccgac
    17641 agccgcactg agaggcagcg gcaaatatat aagtacgccc tcgtaattat cgttggcggt
    17701 aaacaaccgt tggatttcag cgttaacggc tgaaggacat cgggtcaatc gcccaacacc
    17761 aacctcatga aataagtgtg gataccgtct tatggagcaa aacccgcagt cacagctgaa
    17821 acttcttgtc acccgtggta aggagcaagg ctatctgacc tatgccgagg tcaatgacca
    17881 tctgccggaa gatatcgtcg attcagatca gatcgaagac atcatccaaa tgatcaacga
    17941 catgggcatt caggtgatgg aagaagcacc ggatgccgat gatctgatgc tggctgaaaa
    18001 caccgcggac gaagatgctg ccgaagccgc cgcgcaggtg ctttccagcg tggaatctga
    18061 aatcgggcgc acgactgacc cggtacgcat gtacatgcgt gaaatgggca ccgttgaact
    18121 gttgacccgc gaaggcgaaa ttgacatcgc taagcgtatt gaagacggga tcaaccaggt
    18181 tcaatgctcc gttgctgaat atccggaagc gatcacctat ctgctggaac agtacgatcg
    18241 tgttgaagca gaagaagcgc gtctgtccga tctgatcacc ggctttgttg acccgaacgc
    18301 agaagaagat ctggcaccta ccgccactca cgtcggttct gagctttccc aggaagatct
    18361 ggacgatgac gaagatgaag acgaagaaga tggcgatgac gacagcgccg atgatgacaa
    18421 cagcatcgac ccggaactgg ctcgcgaaaa atttgcggaa ctacgcgctc agtacgttgt
    18481 aacgcgtgac accatcaaag cgaaaggtcg cagtcacgct gccgctcagg aagagatcct
    18541 gaaactgtct gaagtattca aacagttccg cctggtgccg aagcagtttg actacctggt
    18601 caacagcatg cgcgtcatga tggaccgcgt tcgtacgcaa gaacgtctga tcatgaagct
    18661 ctgcgttgag cagtgcaaaa tgccgaagaa aaacttcatt accctgttta ccggcaacga
    18721 aaccagcgat acctggttca acgcggcaat tgcgatgaac aagccgtggt cggaaaaact
    18781 gcacgatgtc tctgaagaag tgcaccgcgc cctgcaaaaa ctgcagcaga ttgaagaaga
    18841 aaccggcctg accatcgagc aggttaaaga tatcaaccgt cgtatgtcca tcggtgaagc
    18901 gaaagcccgc cgtgcgaaga aagagatggt tgaagcgaac ttacgtctgg ttatttctat
    18961 cgccaagaaa tacaccaacc gtggcttgca gttccttgac ctgattcagg aaggtaacat
    19021 cggcctgatg aaagcggttg ataaattcga ataccgccgt ggttacaagt tctccaccta
    19081 cgcaacctgg tggatccgtc aggcgatcac ccgttctatc gcggatcagg cgcgcaccat
    19141 ccgtattccg gtgcatatga ttgagactat caacaaactc aaccgtattt ctcgccagat
    19201 gctgcaagag atgggccgtg aaccgacgcc ggaagaactg gctgaacgta tgctgatgcc
    19261 ggaagacaag atccgcaaag tgctgaagat cgccaaagag ccaatctcca tggaaacgcc
    19321 gatcggtgat gatgaagatt cgcatctggg ggatttcatc gaggatacca ccctcgagct
    19381 gccgctggat tctgcgacca ccgaaagcct gcgtgcggca acgcacgacg tgctggctgg
    19441 cctgaccgcg cgtgaagcaa aagttctgcg tatgcgtttc ggtatcgata tgaacaccga
    19501 ccacacgctg gaagaagtgg gtaaacagtt cgacgttacc cgcgaacgta tccgtcagat
    19561 cgaagcgaag gcgctgcgca aactgcgtca cccgagccgt tctgaagtgt tgcgtagctt
    19621 cctggacgat taatcggtag gccggatcag gcgttacgcc gcacccggca ctagtctctc
    19681 tgcacaaacg ccaccttttc gg
//
LOCUS       DAEBXN010000080        18738 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.080, whole genome shotgun sequence.
ACCESSION   DAEBXN010000080 DAEBXN010000000
VERSION     DAEBXN010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18738
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.080"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            377..580
                     /locus_tag="KJL99_002846"
                     /pseudo
     CDS             377..580
                     /locus_tag="KJL99_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
     gene            599..3985
                     /locus_tag="KJL99_002847"
     CDS             599..3985
                     /locus_tag="KJL99_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HBD4437509.1"
                     /translation="MEEAFWAARQGDALLHTSFMADVLGAVLEVAANVVIDALIVGTT
                     SLLATSVGITMGCTAVVLTGFVAGVAMVYTGVTEKVSEACSALANMLFPPQIEGYILT
                     GSGDTWINSKPAARAAATAASRQDIEAQEAQAKAEQEEAQRQEDARTFRDVAGEYLEM
                     AGAIALAVNPVTGPVLMSRALHDQLSTEEGRSEMVDGVTHFVSELWQPTVASAAPGST
                     PTPDDKIDCHKHPSSLTQFLAQKKAAFLDDPLGTALNLLNPGGMLETAFQGVNAVIGS
                     ISNLFKGDDEPPAAEYIAEGARDVRINSQPATRSGARCTCEARVVNEPGNGAFVSPDV
                     RIGGPLLVVRDIRSGRSQITLVATVALMFLRPGKMLSKIACFAASVGMSMLTQKATSA
                     LPHPVNAATGAKYLADDDDFDFSLPGHFPLDWQRVYSSRDERTEGMFGQGWSVMYEVS
                     LVCTPGSADENCMTFVSGMGRRLDMEAVLPGGGFYSPGEGLAVRRGEQGHWLISSDDG
                     QCFLFEADPHHPQRQRLKMLGDRNSNCLNLYYDDRGRIVEISGEQQRPCIRLYYELAA
                     HPRRVTQIYQHFPETAPLLLRRYSYDEAGHLNGVYDSTGHLLREFAYDENHCMTLHRQ
                     PGGEGYYYQWGWYEGPDDAAWRVTGHHTDSGEQYRLAWDLAQRRLCVTDGLGRTRYHQ
                     WDAQNQVTAYQDEAGQVTTFRWSDEERLLLGMTDPQGGKWRYVYDLQGHITETHDPLG
                     RVEQAQWHPVWHQPETEVDAAGNLLGRRAGERATAENSVVPFNRLMSYRGVHYRYDEF
                     GRAVEKEGRSGTQSYRYDAEHRMVEVTTARGTYRYVYDALGRRTEKQHISPDGKPYNR
                     TKFLWDGMRLAQESRPEGTGSLYIYRDQGSYEPLARVDKAGKEGPNRILYFHTDVNGA
                     PEEMTDSDGKIVWETGYQVWGNTIQEKDHGGVEQNLRYQGQYLDRETGLHYNLHRYYD
                     PDVGRFMVTDPIGLRGGLNLYSYAPNPLKYADPLGLTPCAVSNQKANRLLDSSETKVT
                     VRSRSDAEQLFMDRYLGHNYKNMTGESGPSTKNLMEYLTENKTKAGSYHWDDIKDPSV
                     TKPSYRVSGHGPGNPDGDLPHLQVHQHGGSVRHIFFPWET"
     gene            3988..4368
                     /locus_tag="KJL99_002848"
     CDS             3988..4368
                     /locus_tag="KJL99_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4437510.1"
                     /translation="MNNLNTLLSGKVLLKEISPLMPGYRTWIEISLIDELKPSYPFRL
                     DEYAMIGHSPYANECNKDEAKFKLRISSFLASDIDNEYDPSYDYVGKYEIIASLNELK
                     TRLSTLHVNLEQFINSSEDDEYPL"
     gene            complement(4423..4710)
                     /locus_tag="KJL99_002849"
     CDS             complement(4423..4710)
                     /locus_tag="KJL99_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system SymE family toxin"
                     /protein_id="HBD4437511.1"
                     /translation="MAEHDITPEVTISKTQRRYKVGYVRARHQDRKTGIARYYSQHPS
                     LHLKGDWLKEAGFDTGRGVTVKISEGGLTIIADSDEVQELRDGMFSVLNES"
     gene            4839..5348
                     /locus_tag="KJL99_002850"
     CDS             4839..5348
                     /locus_tag="KJL99_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HBD4437512.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            5345..6763
                     /gene="phrB"
                     /locus_tag="KJL99_002851"
     CDS             5345..6763
                     /gene="phrB"
                     /locus_tag="KJL99_002851"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HBD4437513.1"
                     /translation="MTTHLAWFRQDLRLHDNLALAAACRNSSARVLALYIATPRKWET
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCEN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIIDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(6913..8394)
                     /gene="dtpD"
                     /locus_tag="KJL99_002852"
     CDS             complement(6913..8394)
                     /gene="dtpD"
                     /locus_tag="KJL99_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HBD4437514.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            8665..9408
                     /gene="ybgI"
                     /locus_tag="KJL99_002853"
     CDS             8665..9408
                     /gene="ybgI"
                     /locus_tag="KJL99_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HBD4437515.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGMELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            9431..10087
                     /gene="pxpB"
                     /locus_tag="KJL99_002854"
     CDS             9431..10087
                     /gene="pxpB"
                     /locus_tag="KJL99_002854"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HBD4437516.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAYCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGIC
                     "
     gene            10081..10527
                     /locus_tag="KJL99_002855"
     CDS             10081..10527
                     /locus_tag="KJL99_002855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase/urea amidolyase family protein"
                     /protein_id="HBD4437517.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFCQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGD"
     gene            10558..11013
                     /locus_tag="KJL99_002856"
     CDS             10558..11013
                     /locus_tag="KJL99_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allophanate hydrolase"
                     /protein_id="HBD4437518.1"
                     /translation="MEAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQ
                     SNRMGYRLQGQILKRITDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACI
                     IEADMYHLAQIPLGQPIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            11003..11737
                     /gene="pxpA"
                     /locus_tag="KJL99_002857"
     CDS             11003..11737
                     /gene="pxpA"
                     /locus_tag="KJL99_002857"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HBD4437519.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            11773..12564
                     /gene="nei"
                     /locus_tag="KJL99_002858"
     CDS             11773..12564
                     /gene="nei"
                     /locus_tag="KJL99_002858"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HBD4437520.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFPNGLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(12561..13607)
                     /locus_tag="KJL99_002859"
     CDS             complement(12561..13607)
                     /locus_tag="KJL99_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBD4437521.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(13759..14835)
                     /locus_tag="KJL99_002860"
                     /pseudo
     CDS             complement(13759..14835)
                     /locus_tag="KJL99_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            complement(14832..15563)
                     /locus_tag="KJL99_002861"
     CDS             complement(14832..15563)
                     /locus_tag="KJL99_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HBD4437522.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            complement(15578..18037)
                     /locus_tag="KJL99_002862"
     CDS             complement(15578..18037)
                     /locus_tag="KJL99_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBD4437523.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNEQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNISIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNHTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSPNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYSGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            complement(18088..18654)
                     /locus_tag="KJL99_002863"
     CDS             complement(18088..18654)
                     /locus_tag="KJL99_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBD4437524.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLLLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
BASE COUNT         4637 a         4478 c         5164 g         4459 t
ORIGIN      
        1 ttgttgttaa taaaaagctt atagatttat agttgataat taaaacgcat ccgatatttc
       61 agctttttct atgcaagata caattttata aggtgtccat ttttatatgg aggcggtagc
      121 gtgtctgtta ctagtggttt tatttgcata gtgaatatat tgggattgtt aaatacataa
      181 ctatgtatcc gctctggtcg ttgtaaatat tgctaattgc ttaatattat tagtgattat
      241 gactggaaag ataaagtgtg ctgtaaagcg gtgatgggta ttacattcat gattaaaaaa
      301 acagaacctg tcaataaata attcctttat taaaacggac tatttacatt atgacggaat
      361 attatctcaa cgaggcggtt tggaaataca gagccagttt ctccggggta acgagcaggt
      421 gtaccagtgt caggtggcat ttgtattgcc gggtgagcgg gtcatgatgg ccttcaccta
      481 cgccagaacc acaccgctga ccccggcaga tatgacgcgc tgggctgaaa ttaaaaagaa
      541 tctgcgcttc aggatgcggc aggaagtcag gacgaattaa tcatacaggg aacgggacat
      601 ggaagaggca ttctgggcgg caagacaggg tgatgccctg ctgcatacgt cgtttatggc
      661 agatgtgctg ggggcggtgc tggaggtggc cgcaaatgtc gttattgatg ccctcattgt
      721 cggcacaacc tcgttactgg cgacgtcagt ggggatcacg atggggtgca cggccgtcgt
      781 gctgaccggt tttgtggcgg gtgtggcgat ggtgtacacc ggcgtgacag aaaaggtcag
      841 cgaagcctgt tcagccctcg caaatatgct tttcccgcct cagattgaag gttacatcct
      901 caccggctct ggtgacacat ggatcaacag caaacctgcc gcgcgggcag cggcgactgc
      961 ggcatcgcgg caggacatag aggcgcagga agcgcaggcg aaggcagaac aggaagaagc
     1021 gcagcgtcag gaggatgcca gaacgttccg ggatgtggcc ggggaatatc tggaaatggc
     1081 gggggccata gccctggcgg ttaacccggt gacagggcct gtgctgatgt cccgggctct
     1141 gcacgaccag ctcagtacgg aagaagggcg ttctgaaatg gtggatggcg tgacacattt
     1201 tgtgtcggaa ctatggcagc ccactgtggc cagtgcggcc ccgggatcca ccccgacccc
     1261 tgacgataaa atcgactgcc ataaacatcc ttcctcactg acgcagtttc tcgcacaaaa
     1321 aaaagccgcc tttctggatg accccctggg aacggccctt aacctgctga atccgggcgg
     1381 tatgctggaa accgcctttc agggagtgaa tgcggttatt ggcagcatca gtaatctgtt
     1441 caaaggcgat gacgagccgc ccgcagcgga atatatcgcg gaaggcgcgc gggatgtgcg
     1501 tatcaacagc cagccggcga cacgcagcgg ggcacgctgt acctgcgagg ccagggtggt
     1561 gaatgagccg gggaacgggg cgtttgtgtc accggatgtg cgcattggcg gtccgttact
     1621 ggtggtcagg gatatcagaa gcggcaggtc gcagataacc ctggtggcaa ccgtggcgct
     1681 gatgtttctt cgcccgggca aaatgctttc taaaatcgcc tgttttgctg ccagtgtcgg
     1741 gatgagcatg ctgacgcaga aggccaccag tgcccttccg catccggtca atgccgccac
     1801 cggggccaaa tacctggccg atgacgatga cttcgatttc agtctgccgg gccactttcc
     1861 gctggactgg cagcgcgtgt acagcagtcg tgacgaacgt acggaaggaa tgtttggtca
     1921 gggctggagt gtaatgtatg aagtcagcct cgtgtgcaca ccgggtagcg cagacgaaaa
     1981 ctgcatgacc tttgtgtcgg gcatggggcg acgcttggat atggaagccg tactgccggg
     2041 gggcgggttt tacagccccg gtgaagggct ggcggtaagg cgtggtgagc aggggcactg
     2101 gcttatcagc agcgatgacg ggcagtgttt tctgtttgag gctgacccgc accacccgca
     2161 gcgccagcgg ctgaaaatgc tgggcgaccg taacagcaac tgtctgaatc tgtactacga
     2221 cgaccggggc cgcattgtgg aaatcagcgg cgaacagcag cgtccgtgca tccgtctgta
     2281 ttacgaactg gcggcgcatc cccggcgggt gacacagatt tatcagcatt tccctgagac
     2341 tgcaccttta ctgctgcgtc gttacagcta tgacgaggcc ggccacctga acggcgtgta
     2401 cgacagcacg gggcacctgc tgcgtgagtt tgcttacgac gaaaaccact gcatgacgct
     2461 gcaccgccag ccgggcggcg aagggtatta ttaccagtgg ggctggtatg aaggtccgga
     2521 tgacgcggca tggcgggtga cggggcacca cacggacagc ggtgaacagt accggctggc
     2581 gtgggacctg gcgcagcgca ggctgtgcgt gacggacgga ctggggcgca cacgttatca
     2641 ccagtgggat gcgcagaatc aggtgacggc gtaccaggac gaagccggac aggtgacgac
     2701 attccgctgg agcgacgaag aacgactgtt gctggggatg accgaccctc agggcggcaa
     2761 atggcgctat gtgtatgacc tgcaggggca tatcacggag acgcacgacc cgctggggcg
     2821 ggtggagcag gcgcagtggc acccggtgtg gcaccagccg gaaacggagg tggacgcggc
     2881 gggaaacctg ctggggaggc gcgcggggga aagggccacg gcagaaaaca gcgtggtgcc
     2941 gttcaaccgg ctgatgtctt accgtggtgt gcactaccgt tatgacgaat ttggccgtgc
     3001 ggtggaaaag gagggccgga gcggaacgca gagttaccgt tatgatgcgg aacaccggat
     3061 ggtggaagtg acgacagcgc gggggacata ccggtatgtg tacgatgcac tggggaggcg
     3121 gacggaaaaa cagcacatca gccctgacgg taagccgtac aaccggacaa aatttttatg
     3181 ggacggaatg cggctggcgc aggagagcag gccggaggga acaggcagcc tgtacatcta
     3241 cagggaccag gggagttatg aaccgctggc gcgggtggat aaggcgggca aagagggccc
     3301 gaaccggata ctgtattttc acacggatgt gaacggcgcg ccggaagaga tgacggacag
     3361 cgacgggaaa atcgtgtggg agacggggta tcaggtctgg ggtaacacga tacaggagaa
     3421 ggaccacggc ggggtggagc agaacctgcg ctaccaggga cagtacctcg acagggaaac
     3481 ggggctgcac tacaatctgc acaggtacta tgatccggat gtcgggcggt tcatggtaac
     3541 tgacccgata gggttgagag gcggtttaaa tctctacagt tatgcaccta atcctctaaa
     3601 atatgctgat ccacttggat taactccatg tgctgttagt aaccaaaaag cgaatcgttt
     3661 attagatagt agtgaaacca aagttactgt taggtctaga agtgacgctg aacaattgtt
     3721 tatggataga tatttaggtc ataattataa aaatatgaca ggtgagtcag gccccagcac
     3781 taagaactta atggaatatc ttactgaaaa taaaacaaaa gcaggttcat atcattggga
     3841 tgatataaaa gatccttctg taacaaaacc ttcatataga gtgtcaggtc atggaccagg
     3901 aaatcctgat ggtgatttac cacatttaca agttcatcaa catggtggaa gtgttagaca
     3961 tatatttttc ccgtgggaga cataataatg aacaatctca atacattatt aagtggaaag
     4021 gttttgctca aagaaattag tccattaatg cctggatata gaacatggat tgaaatttca
     4081 ttgatcgatg aattaaaacc atcttatcct tttagattag atgaatatgc tatgataggg
     4141 cattcacctt atgccaatga atgtaataaa gatgaagcta aatttaaact acgaatatca
     4201 tcatttttag cttctgacat agataatgaa tacgatccat catatgatta tgttggaaaa
     4261 tatgaaatta tcgcttcact taatgagtta aaaactaggt taagtactct tcatgtaaac
     4321 ttggagcaat ttataaattc atcggaagat gatgagtatc cattataata agacagaata
     4381 aaaagctagg aaggttttaa atcttcccag cttttttaat atttaacttt cgttcagcac
     4441 actaaacatc ccatcccgca gttcctgcac ctcgtcactg tcagcgatga tggtcaggcc
     4501 cccctccgaa atcttcaccg tgacaccccg tccggtatca aatccggcct ctttcagcca
     4561 gtcgccctta aggtgcagac tgggatgttg tgaataatac cgggctatac cggttttgcg
     4621 gtcctggtgg cgggctctga catagccgac tttatagcgg cgctgtgttt tggaaatagt
     4681 gacttctggc gtaatatcgt gttcagccat gattaactac cttgattagt tgcttgcggg
     4741 gagcgcgctt tcgtaatctt gccctgctag cggattaact tcacaaacat catttaccaa
     4801 cgcctacact tactcctgta taccgttcag gagcagtaat gaatctacaa cgatttgatg
     4861 acagcaccct aatccgtatc ttcgcccttc atgagttaca tcgactgaaa gaacatggct
     4921 taacgcgcgg ggcgcttctc gattatcaca gccgctataa actcgtcttt ctggcccatt
     4981 ctcagcccga gtaccgcaaa cttggcccgt ttgtggctga tattcatcag tggcaaaatc
     5041 tggatgattt ttacaaccag taccgccaac gcgtaattgt tttgctttct catcccgcca
     5101 acccgcgcga tcacaccaac gttttgatgc acgttcaggg ttattttcgc ccgcatattg
     5161 attccacaga acgccagcag ctggctgcgc ttatcgacag ttatcgccgt ggcgagcaac
     5221 cacttcttgc gccgctgatg cgtatcaaac actatatggc gctttatcct gacgcctggc
     5281 tttcagggca gcgttatttc gaactttggc cgcgtgtgat taacttgcgc cattcaggag
     5341 ttttatgact acccatctgg cctggtttcg ccaggattta cgtctgcacg ataatctcgc
     5401 actggctgcc gcctgccgca attcgtctgc acgcgtgctg gcgctgtata tcgctacacc
     5461 acgcaagtgg gagacgcata acatgtcgcc gcgtcaggct gaacttatca atgctcaact
     5521 gaatgggcta caaatcgcgc tggcggaaaa aggtattcct ttattgttcc gtgaagtgga
     5581 tgactttgtc gccagtgtcg aaatagttaa acaggtgtgc gcggaaaaca gcgttactca
     5641 tcttttttat aactatcagt atgaggtgaa tgagcgagcg cgggatgtgg acgttgaaag
     5701 agcgctgcgt aacgtggtgt gtgaaggatt tgatgacagc gtgatcctgc cgccgggggc
     5761 ggtgatgacc ggcaatcacg agatgtacaa agtctttacg ccttttaaga atgcctggct
     5821 gaaacggctg cgggaaggga tgccggagtg cgtcgctgca ccaaaagttc gtagtagcgg
     5881 atcgataaag cccgcgccat ccattacgct gaattatcct cgtcagtctt tcgatactgc
     5941 gcatttcccg gtggaagaaa aagcggcgat tgcgcaatta cgccagtttt gcgagaacgg
     6001 tgctggagag tatgagcaac aacgagattt tccggcagtg gaaggcacca gccgtttgtc
     6061 cgccagcttg gcaacgggcg ggttatcgcc tcgccagtgt ttgcatcgct tgttggcgga
     6121 acagccgcag gcgctggacg gtggggccgg tagtgtctgg cttagtgagc tgatctggcg
     6181 cgagttctac cgtcatctga tgacgtatta cccctcgttg tgtaaacatc gtccatttat
     6241 tgcctggacg gatcgtgtgc agtggcaaag caatcccgca catttacagg cctggcagaa
     6301 aggcaaaacg ggatacccga ttattgatgc cgctatgcgt cagcttaaca gcactggctg
     6361 gatgcataac cggctacgga tgattacagc cagttttctg gtgaaagatt tgttgatcga
     6421 ctggcgcgaa ggcgagcgat atttcatgtc gcagctgatt gatggtgatt tggcagctaa
     6481 taacggtggc tggcagtggg cggcttctac gggtactgat gctgcgccgt attttcgtat
     6541 tttcaatccg ataactcagg gcgagaaatt tgaccgtgag ggcgagttta ttcgtcgatg
     6601 gttaccggag ctgcgcgatg taccagggaa agcggtgcat gagccgtgga agtgggcgca
     6661 gaaagcaggt gtgaagctgg attatccgca accgatagtc gagcacaaag aggcgagagt
     6721 acaaacgttg gcagcgtatg aggcggcgcg gaaggggaaa taacagacgc gtcaggcaat
     6781 cgagcccaga tgccggatgc ggcgtgaacg cctaatccag cctacaaatt tgtgcaaatt
     6841 caataaattg caggaaatac gtaggcctga taagacgcgt caagcgtcgc atcaggcatc
     6901 ggtgctggcc tattatgact ccagcgccag cgcgcggttt ctgaatttca gcgcctgata
     6961 cagccaaatc atcagtacca gtcctacaca cgccagtgcg ccccaggtaa tttgatcaaa
     7021 tacttcaata tatgcattga tggagtagtt gatcgccccg gaagcatcaa acgaagcctg
     7081 cgatgtctga tcggcaatca cgcccgccag atagttcgcg atcgcgccag aaagcagcat
     7141 gtagatgccg gttaatacgc cggtcacgcc cggaatttca atgcgcgtaa tttgcgacat
     7201 ggcaaccggg tcgataaaca gttccgcaaa gcccatcacc gcgaggccta acaccatcag
     7261 tggcagagaa gagtgaccat acattgcgga ccagcgggcg cttaaggtca gaatgcagaa
     7321 tccggcgctc atcaggccaa ggccaagagc aaattttccc cagatgcgca cggtacgatt
     7381 acctgcgacg ctttctttaa ccacccacgc caggaacaca ccgcacagca taactgcgaa
     7441 ggcattaatc gactggaaca tcgcggtcgg aacggtataa ccaaacatat cgcggttaac
     7501 gaagcggtcg atataaaggc taatcgagct accgccctgt tgtgcgaagg cccagaacaa
     7561 catactgaag aaggtgagcg tcacaatcag ccccagctcc ttccgctgtt tctggttttc
     7621 tgctttgcga taaatttttg ccagtacacc caggccaata atagtcgcga caattaaggc
     7681 gtataccgac cactctttcc agaacagcac ggtaatcagt gcaggcgttg ccaccagcag
     7741 aaccagcagc catccccagt tcggcaggag aaagtttgtc gcacgcagta cttttttgtt
     7801 aacgccgcgg gtatgagtga aatgacgatt gccacataag aaaatgacca gacccgcgat
     7861 cattccaacc gccgccaggc caaagcccat cgcccagctg tactcttcct gggcgtaacc
     7921 acaggcgata ggtgcgataa tagaccccac gttacccgcc gcatacatca gcgagaaacc
     7981 gccatcacga cgcggatcgg ttggctcata cagctcgccg agcagacagc ttacgttaga
     8041 tttaaacagg ccatagccgc agacgataat cgccagggac agatagagga atgacggatg
     8101 gatctcactg gcacccagca ccacatgacc gatcgccatc aacaacgccc ccagcatcac
     8161 cgccatgcga ttgccgagaa ctttatccgc caaaaagcca ccgaggattg gcgtgacata
     8221 caccagcgaa cagtaggcgc taaataactc gtaggcgtga gtatcgttgt attttagttg
     8281 attggtgaga tagagaatca gcagggcacg catgccgtaa aagctaaaat attcccagat
     8341 ttgcagcgca acgacatagt aaatagcgcg cggttgtgat gcgtgtttat tcataataat
     8401 tccacaattg acggcaacgt cacgtaatag ttgcgcggcg tgcttgtgtt tccttaaagt
     8461 taaaactttg atacagatct gattatttat gcaatatgct gtctgattgc ataaatatac
     8521 attagctgaa gcgtgatgat ataaacaatt tgcgtctttt ggcgtcgggt ttgtttctta
     8581 atatgtcaaa agattggcta tcgacgaaca ggcgatgtaa cgctatgtta acggtgtagc
     8641 tatcagaata cggaaggcgc gaagatgaaa aacaccgaac tggaacaact gattaacgaa
     8701 aaactgaaca gcgcggcgat tagtgattac gcgccgaatg gtttgcaggt ggaaggcaaa
     8761 gagacggtgc aaaaaattgt taccggtgtc accgccagcc aggctttgct cgatgaggca
     8821 gtgcgtctgg gcgcggatgc ggtcattgtg catcatggct acttctggaa aggagagtct
     8881 ccggtcattc gcggaatgaa gcgtaaccgt ttaaaaacgt tgctggcgaa tgatatcaac
     8941 ctgtatggct ggcatttgcc gcttgacgca catcctgagc tgggcaataa cgcacagctg
     9001 gcggcgttac tggggatcac ggtcatgggc gaaattgagc cgttggtgcc gtggggagag
     9061 ctgaccatgc ctgtgccggg aatggaacta gcctcctgga ttgaagcgcg tctgggacgt
     9121 aagccgttat ggtgtggcga taccggacct gaggtggtcc agcgcgtcgc ctggtgcacg
     9181 ggcggcgggc aaagttttat cgatagcgcc gcgcgttttg gcgtggatgc ttttattact
     9241 ggcgaagttt ctgaacagac cattcattca gcccgcgagc agggattgca tttttatgct
     9301 gcaggtcacc atgccactga acgcggtggt attcgcgcat tgagcgagtg gctgaatgaa
     9361 aataccgatc ttgatgtgac ctttattgat attcctaatc ctgcataacg aataatcaga
     9421 gggatcgaaa gtgcaacgag cgcgttgtta tctgataggt gaaacggcgg tagtgctgga
     9481 actggaaccg ccggtgacgc tggctagcca gaaacggatc tggcgactgg cgcagcgtct
     9541 ggtggatatg ccgaatgtgg ttgaagccat tcccggcatg aacaatatca cggtgatttt
     9601 gcgtaatcct gagtcgctgg cgctggatgc catagagcgt ttgcaacgct ggtgggagga
     9661 gagcgaggcc ctggaacctg agtctcgctt tattgaaatt ccggtggttt acggtggtgc
     9721 aggcggaccg gatttggcgg tggtcgcggc gtattgcggg ttgagcgaaa aacaggttgt
     9781 tgaattgcac tcctccgtgg aatacgtggt ctggttttta ggttttcaac cgggcttccc
     9841 gtatctcggg agtttgccgg aacaactaca cacgccacgg cgcgctgaac cgcgcttgct
     9901 cgttccggca ggttctgtcg ggatcggcgg gccgcagact ggtgtttatc cgctggcaac
     9961 gccgggtggc tggcagttga ttggtcatac ctcactcagc ctgtttgatc cggcgcgtga
    10021 cgaacccatc ttattacgtc cgggagacag cgtgcgattt gtaccgcaga aggagggaat
    10081 atgctgaaga ttattcgtgc gggcatgtac accactgtgc aggatggcgg tcgtcacggt
    10141 ttttgccagt cgggtatcag ccactgtggc gcactggata tgcctgcgct gcgcattgct
    10201 aacctgctgg tgggtaatga cgccaatgcc cccgcgctgg agatcacgct cggtcagtta
    10261 acagttgagt tcgaaactga tgggtggttt gccctgacgg gtgcaggttg cgaagcgcgg
    10321 ttggatgata acgccgtctg gaccggctgg cgattgccga tgaaagcagg ccagcgttta
    10381 acgcttaaac gcccgcagca cgggatgcgc agttatctgg cggtcgcggg tggtattgat
    10441 gttccgccgg taatggggtc atgcagcacc gatctcaaag tggggattgg cgggctggaa
    10501 ggccgtttac tgaaggatgg tgactgactc ccgattggca aatcgaagcg tgattttatg
    10561 gaagcgcagg gcgttaaaca gctgctgtgg ggcaaccgca ttcgcgcctt gccggggccg
    10621 gaatatcatg agttcgatcg cgcctcgcag gatgcattct ggcgttcgcc ctggcagctt
    10681 agctcgcaaa gtaaccgaat gggctatcgt ttacaggggc aaattttaaa acgcatcacc
    10741 gatcgcgaac tgttatctca cggtttgtta ccgggcgtgg tgcaggtgcc gcataacggg
    10801 cagccgattg tgttgatgaa cgacgcacag accaccggtg gttatccacg tattgcctgt
    10861 atcattgagg ctgatatgta ccatctggcg caaattccgc tcggtcagcc gattcatttt
    10921 gtccagtgtt cactggaaga ggcactgaaa gcgcggcaag atcagcaacg ttattttgaa
    10981 caattagcgt ggcggctgca caatgaaaat tgacctgaac gccgatctgg gcgaaggctg
    11041 cgccagcgac gcagagctat taacgctggt ttcctctgcc aatattgctt gtggatttca
    11101 tgcaggcgat gcgcaaacca tgcaggcttg cgtgcgtgaa gcaataaaaa atggtgtcgc
    11161 gattggcgct cacccgagtt ttcccgacag ggaaaatttt ggtcgcagcg ccatgcagct
    11221 gccgccggaa accgtttacg cccagacgct gtatcaaatt ggcgcgctgg caacgattgc
    11281 ccgtgcgcaa ggcggcgtaa tgcgtcatgt caaaccgcac ggcatgttgt acaaccaggc
    11341 ggcgaaagaa gcacaactgg cagacgccat cgccagagcg gtatacgctt gcgatccggc
    11401 attggttctc gttgggctgg caggaagcga gctgattcgt gcaggcaagc aatatggcct
    11461 gacaacgcgc gaggaagtgt ttgccgatcg cggttatcag gctgacggct cgctggtgcc
    11521 gcgaagtcag ccaggcgcgt tgattgaaaa cgaagaacag gcgttggcgc aaacgctgga
    11581 gatggtgcaa cacggcagag tcaaaagtat caccggcgaa tgggcaacgg tcgcggcgca
    11641 aacggtctgc ctgcatggcg acggcgagca cgctctggcg ttcgcccgcc gattacgagc
    11701 tacatttgcc aaaaagggga ttgttgtcgc agcataaccc cgcttaataa agaatgaaaa
    11761 aaggatatca ccatgcctga aggcccggag atccgccgtg cagcggataa cctggaggcg
    11821 gcgatcaaag gcaaaccact aactgatgtc tggtttgcct tcccgcagtt aaaaacttat
    11881 caatcacaac ttatcggtca acacgttacc catgtggaaa cgcgtggtaa ggcgttgtta
    11941 actcattttc ccaacggttt aacgctctac agccataatc agctttacgg cgtctggcgc
    12001 gtggttgata ccggcgaaga gccgcagacc acgcgagtat tgcgggtaaa actgcaaacg
    12061 gctgacaaaa ccattctgct ttatagcgcc tcggatattg agatgttgag gccggaacaa
    12121 ctgaccacgc atccgttttt acaacgcgtt ggtcccgatg tgctggatcc gaatctgacg
    12181 ccggaggtgg taaaggaacg gttattgtcg ccgcgctttc gtaaccgtca gtttgcagga
    12241 ttactgctcg atcaggcgtt tctggctggg cttggcaatt atttgcgggt ggagatcctc
    12301 tggcaggttg ggttgactgg aaatcataaa gcgaaagatc tcaatgcggc gcaactggat
    12361 gcactcgcac acgcgttact ggagattcct cggttttctt acgctacgcg ggggcaggtg
    12421 gatgagaata agcatcatgg ggcgctgttt cgctttaagg tttttcatcg tgatggcgaa
    12481 ctgtgcgaac gctgtggcgg catcattgag aaaaccacgt tgtcctcgcg cccgttttac
    12541 tggtgtccag gctgccagca ctaggccgac cgcttcggcg cataggttga aataaaccgc
    12601 gcaatggctg gccccgtcag caaaatactg aacaggcgca gggtttgcat cgccataata
    12661 agcgccatat cggcattgct ccctgcggcg atgaccgcca cggtatcaag cccgccggga
    12721 ctggtggcga ggtaggcagt cataaaatca atatgcataa accgggtcag tccccacgcc
    12781 atacctgcac aaatagccag cagagcaaaa atcgacagca ggatttgcgg tagcgggcgt
    12841 agtgcccgca gtaagatttg tttatcgaaa ccaagaccaa tccgccagcc aattgccata
    12901 tacgccatcg ccagcagcca ttccggtagt tcgatggtga tgagctgacc agactggagc
    12961 actgcgcccg ccagcatcgg gatgagcatc gtgccggaag gaagacgcag catgcatccc
    13021 gccgtaccgg caacgactgc gagcaaaatc gtaagcagga gattaatgct cactggcggg
    13081 aaccagacga tttgctggtt aaccgcttca gcgttatcac ccagcatcat gcgggtgacc
    13141 agaacagcgg cccccgcgac gaacagcact cgcagatatt gcataaacgc caccaggcga
    13201 atatctgcgc cgtaatcttg cgccatagcg accattgccg ctgcgccgcc tggagaagaa
    13261 ccccaggcac cggtatttcc aggcagtgag ctatagcgca ccaataacca acccacgatg
    13321 gcgctggaaa gcagcgtcac cagtaaaata gcaagcacga tcggccagtt ggcggccagc
    13381 gtggtgagaa tcgaaccagt gaggttttgc gcaatcatgc agccaagaat ggcctgcgcg
    13441 gcgagaaaag cggagcgggg aagttgcaga gtaattccgc gcatactgaa gatgatcccg
    13501 gcgatcatcg gcccaagtaa tagcgccgcc gggagatgta ccgcgaggaa accaatagaa
    13561 aggagaagtg atagcacaca taacattccc cactgcaaaa ctggcatcct tcgctccctc
    13621 attattttct ctttggtgta tcgatacagc ataaagaaag ccgggctagg aaaaaataac
    13681 agcaatcaag gaaaagggga aaatcagcaa ttttctgaaa gagatgccct ttccggctgc
    13741 aaagggcatc atggcaaatc agttatagtt aattttaaaa agcaccactg tatcgaaggg
    13801 cccagcctcg atgggggtac ctgcaatgga cgaaagttca gctgtatagg ttttttcgta
    13861 gaccaacatg tcgctggtga aatcggcgta ttcagcatat ttattgaagg tataaggcga
    13921 ggcgttttca tccagcaaac gtagcttcag accattacca atcaacaatg ctttatcttc
    13981 ttcaaccaac gtttcttcgg tataaaagga ggaactgact ttaaatccgt cggtgcattg
    14041 gtcattttgt gattttgtcg tcttaatact gaatgttttc gacattgtgt ggcgacgaat
    14101 atccagcaaa ttaaatttac caaaatcaat catctgtgtt tccggtgaaa tggaaaaatt
    14161 gacgctgcaa tccagcacgc gaatgttttc cagaccagta atatgatatt tcaggttttg
    14221 ggcagtaggg tcttcgttaa cgctaccggc accgtcaaat tgcacgacga tatagtcgct
    14281 aagcgtactt tgataatcat gcggcggcat ttctcgtatt ttgacgtata accgcatgcg
    14341 agcgagaaag gttcgcgaca tgtgaatatt agcgggatca cccgagcaaa ttttgttctg
    14401 ccatcccatc aggggtataa atgtcaatgt tcttactgtc gatacactgg ttagtgtcga
    14461 tgcggctttt tccctggttg gcgtcataat caacgccatt gtaagttact ccgagttgat
    14521 aataacgatc ctgcaccccc gggtaggggt ttacccatac gtagacatgt tcgctttcaa
    14581 agttgccgtt ggtattgttg tcacaataga cgggaatttt aatatcgtca gattcccaga
    14641 tcttatttta atatcgtcag attcccagat cttatcgccg agtttggcat tgcctggtac
    14701 agcaaaaggt tgaatcgctt ctgatttttc taccgagcca ccggatgaac caaagtagca
    14761 gttcagggca agcgcagact tcaacgacag aaataacaga cagatgacga gcaacactcc
    14821 cttgccagca ctcatttctt cgcctccacg gcttgtaaat ggcattgtcc gctgcagttg
    14881 agcgtcgtca tgcgtaacgc gccgtaatca tccatataac cgagataaaa ctggctgccg
    14941 ctataatttc cggtgtttgt gttgaccgta ctaaaaggcg caaccatcac ggttttgaag
    15001 ccggggagaa cgcctttttc atttcgtccc aggtaggcaa tggtcagata atacgcggta
    15061 ggatttttca gtgtcagttg gttgccctgc tggctgacct gtaactgcag ttcgaccttt
    15121 tctcctgctt tcttgcgtaa tgcagccggt cgccagaaca ccttgatacg actctgaatg
    15181 gcgacttgca gaacggcatg gtcgctgttt ttttccgggg cgggaggaat ttcacgcata
    15241 ttgtagaaaa atagcgtttc gcgatcgccc ggtaattttg tggtcgaggc ttgctttact
    15301 atccgtacct gtgttgttgc tttcggttcc agacgttgta tcggcggcag ggcgaccaga
    15361 agatcatcgc ttttctcccc tttttcattt tcgagccagg aataagcgag gtaggggagt
    15421 ttatcgctac gattatccac ccgcagactg gtagctttat cattagcgtt aaacacaata
    15481 cgagtgcgat cgggttgtag tgcagcgtgg ctgcacagac tggcaaccaa caacaaaagg
    15541 ggtagtcctt tcataaatgt cattttcatt tccttaatta atgacaaggt aaaatcaggc
    15601 gatttgcggt gtcttccata tgttcaggaa gatggagcga gcaatgctgg ctatcacccc
    15661 agaccacggt aaacttttga ttttcagcga ctccgcttaa ccaggcatgt ccttcctcgc
    15721 caaccatccc cacgctaatg ccgctgtcat cctggcggat atccgctccg aggggaggaa
    15781 actgaccgct ggcgttgcga atgatgacgt taacgtcttt accggaacgg gaagccagtg
    15841 atttgtaacc tatcgcgcct tcaatccacg tttctttgat aacgttttct gcaactgtta
    15901 cgccgtcggg taagtcattc atgttcaccg ccacggtgga aggctggtaa ctggaaatca
    15961 acggcaccac cgcaatgcca aaatggttgg tgtagtcgag attgccctga accggaatat
    16021 ctgccacgcc atcggtgctg accatcaggc gtggttcatt ggtggagctg cggcgatgaa
    16081 acgctgcacc atattgggtt gcggtgaaag aaccgctcca gctgctgctg acggaactgt
    16141 aatcattggc ggcataggta ccagaaatat cccactcacc cgctgaactc aggtgctgat
    16201 agttaccgct cacctgggct ccattgtcag gacggtcgga ttgcagtccg gcagacatgc
    16261 cccagctatt acgttcatcc agcgtatcgt tccacgacat gcggtgtgtg gtgctgtggc
    16321 tactgttttg catgtcatag ccaacccgac caccgttacc gaaggggagc gaaatcgaca
    16381 ggtaaatctg gttgtcgcga tctttatctt cgtaatgggt tgtattgaat gacgtagaaa
    16441 tagagatatc tctccagtca ccaatatcaa cattaaaacc tgccgtgatg ttggcggtcg
    16501 tggaggcgtc tgcattccac caggtttgat gtagcaggtt ggcgtaaaga ttgaggttta
    16561 gtggggtaat cggttggccc acagataagc tgatcgtctg tttttcgtcc tgcgcatcgc
    16621 tgtcgttgta tttgtgatcc agatagttgg cgtagctgtg aaaatgacga tcggagaaac
    16681 gataagcggc gagggaaatc gtgctgttag tggcatccac ttgtttgctg taattaaaac
    16741 ggtagcttaa gccccgctcg tcctgctgag tatcaaaatg gctactggcc cacgtgacat
    16801 caaacgacag cgcaccaagc cacagcatat tttgcccaat acccattgcg gcagaatggt
    16861 agtcatcacc agaaagcagc aggccgctgt acagcgaggt gtttgacagc atcccccagg
    16921 aaacttcatt gctaaaaaag gtttcatttt cagtttgatg tgacatggag ggccgtggct
    16981 gacccgcggc cagtttatag cgaacctgtc cctgacgagt caggaagggc gtcgatgctg
    17041 ctgaaacctg gaaattgttc acccgaccat cttcttccgt cactttgaca tccagtgtgc
    17101 cctgaacaga ctgattaagg tcgtcaatga taaatgggcc tggtgggact tttttctggt
    17161 aaatgacgcg gcctgattga ctgatcgtca ccgtggcatt ggtctgtgca ataccgctaa
    17221 tttgtggggc gtagccgcgt agttcccatg gcaacattcg atcgtcactt gccagtgccg
    17281 cgccggtata agaaaaaccg tcgaaaatat tgggactaaa atccgtttcg ccgagggtta
    17341 attttgaacc taattgcggt aacgggcgaa aaagataggt acgtgatatt tcgcctgact
    17401 gttcatggtt atcatcgcta tcagtatgat tcaactgata atcactgcgt aagcgccatg
    17461 cccccgtgtt gattccggct gttccatagg cgttaagatt agtgctgctg ctgccatcct
    17521 gtgggcggta gctgctggca aacaagttgt aatccatcag gacaccggcg acaccttctt
    17581 tccatgtgga gggtggggtc cagttttctg agtgccacgc cagccaggct tgcggaatac
    17641 taatattcag ttgctgattg gcttgatcga aattgaagag catttcaggt cgggagctaa
    17701 aatcgacgca ctgatttatc aatggtaacg actgacggac ttcaggtttt aagccaaatt
    17761 tatcgaccag taaatcattg atgcacggaa tggttttatc gtcatttttg tgccagttaa
    17821 tcttttgctc gttactgatt tggttattat ttaccgcaac gctaacaaaa tactcaccgg
    17881 gagcaatgac tcctttttcc tttaataatg aaatatcaat gcggtcgcgc attgatttat
    17941 cgagaacatt cagattgaat tcgaccgcca atgcgcaagg catcgccacg accaggcagg
    18001 atacaaaaga gagtcgataa atattcacgg tgtccatacc tgataaatat tttatgaaag
    18061 gcggcgatag cgccgccaaa gtaatactta tttataatcc agcacgtagg ttgcattcgc
    18121 cgtaactgcg cctggcgtta caggtgtagc ctgatcaatt tgttccatcc aggcaaagaa
    18181 attcagcgtt tgttcgctgg aggtcggagc caggtcgata tctggagtag cggtgcctaa
    18241 aacgatattg ctttgatctt tattcattaa acgtacgccg acgccggtgg cttcacccgt
    18301 gctggtgttg ttgagaatcg gatctgcgcc ggtggttttc gctgagctat caaaggtgac
    18361 tgcaacttta gaaatcttgc caccggaacc gttgctggag ttttccagat cacagttgac
    18421 taagagcagg tcgacagcaa cggcatcgct gtgatgtgac tgattaatgt ggctggtggt
    18481 cacctgaccg agttcaacct ctttatcaat atcatcctga tgtatttcac acggtgcttc
    18541 aataacttca cctttaaagt gaatttcacc ggatccttca tcagccgcat aaacaggtgc
    18601 tgtgaacagc agagatacgg ccagtgcggc caatgttttt tgtcctttaa acataactga
    18661 gtcctttaac agaaaagaaa aaagtatatc ggtgcaagaa tatcttattt gattgtcgaa
    18721 tgtttttagt ttttctta
//
LOCUS       DAEBXN010000081        18736 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.081, whole genome shotgun sequence.
ACCESSION   DAEBXN010000081 DAEBXN010000000
VERSION     DAEBXN010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18736
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.081"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(<1..1848)
                     /gene="traD"
                     /locus_tag="KJL99_002864"
     CDS             complement(<1..1848)
                     /gene="traD"
                     /locus_tag="KJL99_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HBD4437525.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILIGLV
                     LWVKISWQTFINGCIYWWCTSLEGMRDLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLATVVALVICLITFFVVSWILGRQGKQQSENEVTGGRQLTDNPKDVA
                     RMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVSTGKSEVIRRLANYARKRGDMVVI
                     YDRSCEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNVANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHNGEPFTIRDWMRGVREDQKNGWLFISSNADTHA
                     SLKPVVSMWLSIAIRGLLAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCY
                     VFGIQSYAQLEDIYGEKAAATLFDVLNTRAFFRSPSHQIAEFAAGEIGEKEHLKASLQ
                     YSYGADPVRDGVSTGKEMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQTRP
                     KVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPEVASGEDVTQA"
     gene            complement(1899..2636)
                     /locus_tag="KJL99_002865"
     CDS             complement(1899..2636)
                     /locus_tag="KJL99_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4437526.1"
                     /translation="MTSDNIAEYIGENNQFTEKPATEYPPVIQDNTVTTKDYLYSNNI
                     DTDLLVVIGGLIVILFLMMLIMYFINSLRHKELTEILTKISFPEKSGSNLLKPEAVSC
                     NLLSLCVLAPVFCTPRKLAEVSREIIVASAQEIAWTPGSSRIDGHQAENITWAISSQS
                     PADKYDLINKTSCVSPDSGRFYEPDTVLDHRERVRLFIQIQFPHGSDWSEVLRLTDIV
                     TEKVSSGRTAGGHHHRHTGYTWSVSVN"
     gene            complement(2839..3570)
                     /locus_tag="KJL99_002866"
     CDS             complement(2839..3570)
                     /locus_tag="KJL99_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HBD4437527.1"
                     /translation="MKTKKMMMVALVSSTLALSGCGAMSTAIKKRNLEVKTQMSETIW
                     LKPASERTVFLQIKNTSDKDMSGLQGKIADAVKAKGYQVVTSPDAAYYWIQANVLKAD
                     KMDLRESQGWLNRGYEGAAVGAALGAGITGYNSNSAGATLGVGLAAGLVGMAADAMVE
                     DVNYTMITDVQIAERTKATVTTDNVAALRQGTSGAKIQTSTETGNQHKYQTRVVSNAN
                     KVNLKFEEAKPVLEDQLAKSIANIL"
     gene            complement(3584..4093)
                     /gene="traS"
                     /locus_tag="KJL99_002867"
     CDS             complement(3584..4093)
                     /gene="traS"
                     /locus_tag="KJL99_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003898910.1"
                     /note="TraS, as the term is used in F-like plasmids, is a
                     plasmid entry exclusion protein. It interacts with the
                     F-like plasmid mating stabilization protein TraG; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer entry exclusion protein TraS"
                     /protein_id="HBD4437528.1"
                     /translation="MITHYDIKTETQKLKDVLSVEGVNIPPLLQVIKPGVYVFLWVLL
                     WPTFLRLLADKVDIRDAGFDICFSGVMGFILFVAITNGMMLYLAIPKKFRDESKVISF
                     MYDKNKTYILSFVIVFSIVSFAHTFLFGFLSITLFVIISFIYTIDINRYNLSAIVSVI
                     GLFKKESVS"
     gene            complement(4090..6915)
                     /gene="traG"
                     /locus_tag="KJL99_002868"
     CDS             complement(4090..6915)
                     /gene="traG"
                     /locus_tag="KJL99_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating pair stabilization
                     protein TraG"
                     /protein_id="HBD4437529.1"
                     /translation="MNEVYVIAGGEWLRNNLNAIAAFMSTRTWDSIEKIALTLSVVAV
                     AFMWVQRHNVMDLLGWVAVFVLISLLVNVRTSVQIIDNSDLVKVHRVDNVPVGLAMPL
                     SLTTRIGHAMVVSYEMIFTQPDSVTYSKTGMLFGANLIVKSTDFLSRNPEIINLFQDY
                     VQNCVLGDIYLNHKYTLEDLMASADPYTLIFSRPSPLRGVYDNNNNFITCKDASVTLK
                     DRLNLDTKTGGKTWHYYVQQIFGGRPDPDLLFRQLVSDSYSYFYGSSQSASQIMRQNV
                     TINALKEGITSNAARNGDTASLVNLATTSSMEKQRLAHVSIGHVTMRNLPMVQTILTG
                     IAIGIFPLLVLAAVFNKLTLSVLKGYVFALMWLQTWPLLYAILNSAMTFYAKMNGAPV
                     VLSELSQIQLKYSDLASTAGYLSAMIPPLSWMMVKGLGAGFSSVYSHFASSSISPTAS
                     AAGSVVDGNYSYGNMQTENVNGFSWSTNSTTSFGQMMYQTGSGATATQTRDGNMVMDA
                     SGAMSRLPVGINATRQIAAAQQEMAREASNRAESALHGFSSSIASAWNTLSQFGSNRG
                     SSDSVTGGADSTMSAQDSMMASRMRSAVESYAKAHNISNEQATQELASTGSKFSYGGY
                     ADAHAEWGIKPGILGVGGGVGVRGGFKGSLDRTDDDSHSASSGSRATHDARHDIDAKA
                     SKDFKEASDYFTSRKVSESGSHTDNNADSRVDQLSAALNSAKQSYDQYTTNMTRSHEY
                     AEMASRTESMSGQMSEDLSQQFAQYVMKRAPQDAEAILTNTSSPEIAERRRAMAWFFV
                     QEQVQPGVDNAWRESRGDIGKGMESVPSGGGSQDIIADHQGHQAIIEQRTQDSNIRND
                     VKHQVDNMVTEYRGNIGDTQNSIRGEENIVRGQYSELQNHHKTEALSQNNKYNEERES
                     QKIIPEPSEEAVKKMVDDKKDRLKGPL"
     gene            complement(6912..8285)
                     /locus_tag="KJL99_002869"
     CDS             complement(6912..8285)
                     /locus_tag="KJL99_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraH"
                     /protein_id="HBD4437530.1"
                     /translation="MLRIKPLLVLCAALLTVTPAASADVNSDMNQFFNKLGFASNTTQ
                     PGVWQGQAAGYAYGGSLYARTQVKNIQLISMTLPDINAGCGGIDAYLGSFSFINGDQL
                     QRFVKQIMSNAAGYFFDLALQTTVPEIKTAKDFLQKMASDINSMNLSSCQVAQGLVGG
                     LFPQTQESQQKVCQDIGSESNIFADWAASRQGCTVGGQSSSVRDKASDKDKERVLKNQ
                     NIIWEALKKNRMFDGNKELKEFIMTLTGTLIFGEDSEITPLPARTTDRDILRAIMEGG
                     TAKIYHCNDSDKCLKVVADANVTIARDKALKSQITKLLTSIQNKAVSDTPLDAREQGF
                     ISSTTIPVFKYLIDPQMLGVSNSVVYQLTDYIGYDIMLQYIQELLQQARAMIATGNYP
                     QAVMDNVLENLNQAQQQIAIFQSQVQVQQDALLVVERQMNYMRQQLSARMLSRYQNNY
                     HFGGNSL"
     gene            complement(8272..8697)
                     /gene="trbF"
                     /locus_tag="KJL99_002870"
     CDS             complement(8272..8697)
                     /gene="trbF"
                     /locus_tag="KJL99_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer protein TrbF"
                     /protein_id="HBD4437531.1"
                     /translation="MNHQKYQRELMMKEKINDTEPGIKQIEREIERGCDNAKKYFWLF
                     VVFFAAGLIVRNVMHDFFSAGIDSWKADPELNNFRYMWNTLMYVIPIMLYALAAGFLA
                     AASLSPLCEIIFGGVRIFLLKRRMRRENTLREGSNNASH"
     gene            complement(8645..8992)
                     /gene="trbJ"
                     /locus_tag="KJL99_002871"
     CDS             complement(8645..8992)
                     /gene="trbJ"
                     /locus_tag="KJL99_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HBD4437532.1"
                     /translation="MPPVLWRGWIPFCRYTERKKVRNKQVVLLIAGISGIATGIIVSL
                     NIPFIRQGLFYPASPVEIVVSLCLTFSVSVVFFVGAIVGWISVSEIYYSRMTCLHESS
                     EISEGTYDERKNK"
     gene            complement(8922..9467)
                     /gene="trbB"
                     /locus_tag="KJL99_002872"
     CDS             complement(8922..9467)
                     /gene="trbB"
                     /locus_tag="KJL99_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HBD4437533.1"
                     /translation="MSLTKLLPVILLMMATGVQASTRDEIERLWNPQGMAVQPAQPAA
                     DTSARTEKPAPRWFRLSNGRQVNLADWKVVLFMQGHCPYCHQFDPVLKQLAQQYGFSV
                     FPYTLDGQGDTAFPEALPVPPDVMQTFFPNIPVATPTTFLVNVNTLEALPLLQGATDA
                     ASFMARMDTVLQIYGEEKGAK"
     gene            complement(9454..9738)
                     /gene="traQ"
                     /locus_tag="KJL99_002873"
     CDS             complement(9454..9738)
                     /gene="traQ"
                     /locus_tag="KJL99_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /protein_id="HBD4437534.1"
                     /translation="MISKRRFSLPRLDITGMWVFSLGVWFHIVARLVYSKPWMAFFLA
                     ELIAAILVLFGAYQVLDAWIARVSLEEREALEARQQAMMEGQQEGGHVSH"
     gene            9819..10154
                     /locus_tag="KJL99_002874"
     CDS             9819..10154
                     /locus_tag="KJL99_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin ArtA"
                     /protein_id="HBD4437535.1"
                     /translation="MKKLSFKEKLEIVRNIIRESLSGNAAFALLVYSLLHSLPIKTFA
                     DLFVTILLFIAIIAVVIWLFLIIYIYFCELFRSHWIAVWFILWSVVINLTIGFGIYDK
                     LAPVISLMK"
     gene            complement(10135..10476)
                     /gene="trbA"
                     /locus_tag="KJL99_002875"
     CDS             complement(10135..10476)
                     /gene="trbA"
                     /locus_tag="KJL99_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbA"
                     /protein_id="HBD4437536.1"
                     /translation="MHTGDLCFFTGASLFIVGVVGYFFTGPNNKGVYLLSSFVFFVVN
                     VISVCMLPDNFLFLCGAVMNQCAVPVVSMAVSTIPVITKFDSFRRMFVCIMLSAIWSG
                     VMWFFIISLVK"
     gene            complement(10490..11233)
                     /gene="traF"
                     /locus_tag="KJL99_002876"
     CDS             complement(10490..11233)
                     /gene="traF"
                     /locus_tag="KJL99_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HBD4437537.1"
                     /translation="MNKALLPLLLCCFIFPASGKDAGWQWYNEKINPKEKENKPVPAA
                     PRQEPDIMQKLAALQTATKRALYEAILYPGVDNFVKYFRLQNYWTQQAGLFTMSAKKA
                     MLAHPELDYNLQYSHYNGTVRNQLAADQAQQRQAIAKLAEHYGIMFFYRGQDPIDGQL
                     AQVINGFRDTYGLSVIPVSVDGVINPLLPDSRTDQGQAQRLGVKYFPAMMLVDPKQGS
                     VRPLSYGFISQDDLAKQFLNVSEDFKPNF"
     gene            complement(11226..11483)
                     /gene="trbE"
                     /locus_tag="KJL99_002877"
     CDS             complement(11226..11483)
                     /gene="trbE"
                     /locus_tag="KJL99_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HBD4437538.1"
                     /translation="MKVIFTSNRLVDFLIRLSITAIVISPVIIWSWDTVKETTADGIL
                     AAAFVILYSGVLLFILYFCFSALTGLQKDDERKNDERNEDE"
     gene            complement(11510..13339)
                     /gene="traN"
                     /locus_tag="KJL99_002878"
     CDS             complement(11510..13339)
                     /gene="traN"
                     /locus_tag="KJL99_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000826164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HBD4437539.1"
                     /translation="MVFFLSFSVFADSNSDYRAGSDFAHQIKGQGSSSIQGFKPQESI
                     PGYNANPDETKYYGGVTAGGDGGLKNDGTTEWATGETGKTITESFMNKPKDILSPDAP
                     FIQTGRDVVNRADSIVGNTGQQCSAQEINRSEFTNYTCERDTMVEEYCTRTASITGDW
                     KYTDEYREVTIPHSQFRFSMNGLKLVFSVTAPVTGTVESASLSVYAAFFFLNSRYTFM
                     NTTFNVGLANGQSDTYPLSGATGLQVTQGQVLTGSGCTANGNCLPHGNGDRKVYESLV
                     SGASTFTLKLRMKVRDKEWVPRVEWVESCPFNKADGVLTGTECSEPGGTKTGVMEGKP
                     WNITQACWAYRDKYVTQSADNGTCQKYVDNPACTLASRQCAFYSDEGTCLHEYATYSC
                     ESRTSGKVMVCGGDVFCLDGECDKAQSGKNSDFGEAVSQLAALAAAGKDVAALNGVDV
                     RAFTGEAKFCRKAAAGFSNCCKDGGWGQDVGLAKCNSEEKALGKAKDNKLTVSVGEFC
                     SRKVLGVCLQKKRSYCQFDSKLAQIVQQQGRNGQLRIGFGSAKHPDCRGITVDELQKI
                     QFDRLDFTNFYEDLMNNQKIPDSGVLTQKVKEQIADQLKQAGQ"
     gene            complement(13650..14030)
                     /locus_tag="KJL99_002879"
     CDS             complement(13650..14030)
                     /locus_tag="KJL99_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4437540.1"
                     /translation="MTLPAGYYQIDPEIRALVAAMNIHGFRTYASCQGHGFPVTKLPP
                     YIAFACPVKMAALLEQRLRQDAESAIPRLAWGWSVKGAFNSKFQLCFRLQPDTPHYWY
                     NRYCRHSLCADFRTLISLLKSLSE"
     gene            complement(14027..14665)
                     /gene="trbC"
                     /locus_tag="KJL99_002880"
     CDS             complement(14027..14665)
                     /gene="trbC"
                     /locus_tag="KJL99_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HBD4437541.1"
                     /translation="MNRKIFALFALTAGMHLAVHASENVNTPENRQFLKQQENLSRQL
                     REKPDHQLKAWAEKQVLENPLQRSDNHFLDELVRKQQASQDGKPRQGALYFVSFSIPE
                     EGLKRMLGETRHYGIPATLRGMVNNDLKTTAEAVLSLVKDGATDGVQIDPTLFSQYGI
                     RSVPALVVFCSQGYDIIRGNLRVGQALEKVAATGDCRQVARALLNNPGDKQK"
     gene            complement(14674..14979)
                     /locus_tag="KJL99_002881"
     CDS             complement(14674..14979)
                     /locus_tag="KJL99_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4437542.1"
                     /translation="MATMDSKYIQHLRKISAVLLRLISRTLKLFPFGLVMVPVILFYF
                     APDSFLAEIIHEWKKADISEQVTLVRLYLKVTFTCAFMAVFLNYLFSDEFMPVNSRK"
     gene            complement(15009..16001)
                     /locus_tag="KJL99_002882"
     CDS             complement(15009..16001)
                     /locus_tag="KJL99_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HBD4437543.1"
                     /translation="MKRSLWLLMLFLLAGHVPAASADSACEGRFVNPITDICWSCIFP
                     LSLGSVKVSQGKVPDTANPSMPIQICPAPPPLFRRIGLAIGYWEPMALTDVTRSPGCM
                     VNLGFSLPAFGKTAQGTAKKDEKQVNGAFYHVHWYKYPLTYWLNIITSLGCLEGGDLD
                     IAYLSEIDPTWTDSSLTTILNPEAVIFANPIAQGACAADAIASAFNMPLDVLFWCAGS
                     QGSMYPFNGWVSNESSPLQSSLLVSERMAFKLHRQGMIMETIGKNNAVCNEYPSPILP
                     KERWRYQMVNMYPDSGQCHPFGRSVMRWETGKNPPNTKKNFGYLMWRKRNCVFL"
     gene            complement(15998..16630)
                     /gene="traW"
                     /locus_tag="KJL99_002883"
     CDS             complement(15998..16630)
                     /gene="traW"
                     /locus_tag="KJL99_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HBD4437544.1"
                     /translation="MRCRGLIALLIWGQSVAAADLGTWGDLWPVKEPDMLTVIMQRLT
                     ALEQSGEMGRKMDAFKERVIRNSLRPPAVPGIGRTEKYGSRLFDPSVRLAADIRDNEG
                     RVFARQGEVMNPLQYVPFNQTLYFINGDDPAQVAWMKRQTPPTLESKIILVQGSIPEM
                     QKSLDSRVYFDQNGVLCQRLGIDQVPARVSAVPGDRFLKVEFIPAEEGRK"
     gene            complement(16627..17013)
                     /gene="trbI"
                     /locus_tag="KJL99_002884"
     CDS             complement(16627..17013)
                     /gene="trbI"
                     /locus_tag="KJL99_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HBD4437545.1"
                     /translation="MTTTQKTTDVTAPRRSHWWWTVPGCLAMVLLNAAVSYGIVRLNA
                     PVTAAFNMKQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            complement(17010..>18736)
                     /gene="traC"
                     /locus_tag="KJL99_002885"
     CDS             complement(17010..>18736)
                     /gene="traC"
                     /locus_tag="KJL99_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HBD4437546.1"
                     /translation="ILTLTLVVEDQVKTHSEANLKYMDLEKKSKTSYAKWFPSVEKEA
                     KEWGELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSFRKNGFELISPRF
                     NHMRNFLTCLPFMAGKGLFKQLKEAGVVQRAESFNVANLMPLVADNPLTPAGLLAPTY
                     RNQLAFIDIFFKGMNNTNYNMAVCGTSGAGKTGLIQPLIRSVLDSGGFAVVFDMGDGY
                     KSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSVMASPNGNLDEVHEGLLL
                     QAVRASWLAKENRARIDDVVDFLKNASDSEQYAGSPTIRSRLDEMIVLLDQYTANGTY
                     GQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENRMYRTPRTLKKLN
                     VIDEGWRLLDFKNRKVGEFIQKGYRTCRRHTGAYITITQNIVDFDSDKASSAARAAWG
                     NSSYKIILKQSAKEFAKYNQLFPDQFQPLQRDMIGKFGAAKDQWFSSFLLQVENHSSW
                     HRLFVDPLSRAMYSSDGPDFEFVQQKRREGLSIHEAVWQLAWKKSGPEMASLEAWLEE
                     HEKYRSVA"
BASE COUNT         4611 a         5086 c         4351 g         4688 t
ORIGIN      
        1 agcctgagtc acgtcttctc cggaggcaac ctccggttcg aagaggctgg ccatctggcg
       61 accttctgct tcccttgcag caagtacggc actcagacgg ttctccattt ccgggttgat
      121 gtcacgcgga ataaactccg gagcgacctt cggtcgtgtc tgatatttca gtgagagttt
      181 tactgccgga tacggtccgg gcagagtgac ataacaggtc agatccggca gagactgaat
      241 gtcggaataa ctgaccagcg tctggcgctc catctcctta ccggtcgata ccccgtcacg
      301 cacggggtca gcaccgtagg aatactgcag actggctttc aggtgctctt tctcaccaat
      361 ttcaccggca gcaaactctg caatctgatg gctcggggag cggaaaaagg cacgggtatt
      421 cagcacatca aacagcgtgg ccgccgcttt ctcaccgtag atatcttcca gctgggcata
      481 ggactggata ccaaacacat aacagcctcc gaacttacgg gcttccggca ggatctccac
      541 caggtccggc agtttgtgta acgtgggtaa ctcatcacag aaaaaccaca cacgacggtt
      601 acggttttct cccattgcca gcagaccacg aatggcaatg gacagccaca tggaaaccac
      661 cggcttcagg gaggcatgag tgtcggcatt tgacgagata aacagccagc cgtttttctg
      721 atcttcccgg acaccccgca tccagtcacg tatggtgaag ggttcaccgt tatgctcaat
      781 cccctgcagg taacggatgg ctttcacgta gttggtcagc acagcacgga tggaaatcgc
      841 cgttttctca attttctctt ccaccaggtt ggccgccggt gaattacgca ggaaggtacg
      901 cagtttttcg attttgatgg aaagcagtgt gtccaccagt ttgctgtagc tgcggttggg
      961 gtcattacgc atcagatacg ccgcttccgc aaaaatggta cgtcctgatc cctgccagaa
     1021 cgggtcctct ttcgttccca tcgggatcag cgtgttcgcg acattatcaa aatccggctg
     1081 tgtcaggcac tccttccaca aatcccaggc ggcacagcgg gcatccagcg gattcaggat
     1141 cttatcgatg gagggatcat agtaactttt aacaaactca cacgaacggt cataaatcac
     1201 caccatgtcg ccacgtttac gggcatagtt ggcaagacgt cgaataacct ctgacttacc
     1261 cgtcgacact gttccgtgca gacagaagtt ctggatttcg gaatcccgga taatcggcag
     1321 atcgccaatc cggatatcgg agtccttgcc gtcttttttc agcatccggg caacgtcttt
     1381 cggattgtct gtcagctgac gaccacctgt gacttcattt tcgctctgtt gtttaccctg
     1441 acgccccaga atccaggaga caacaaagaa ggtaatcagg caaataacca gtgccacaac
     1501 tgtagccaga acgaatgcgg accatagctg ctctccgcac cagatcatat atttatcatg
     1561 cagtacctga gcagcgttca tccggaaggt tttgccgtaa tactggatct catagaccgg
     1621 ctgggatttt attaaatccc gcatgccttc cagtgacgta caccaccagt aaatacagcc
     1681 gttgataaac gtctgccagc ttatttttac ccataagacc agaccaatga ggatccagaa
     1741 aaaaataaac aggcagtaaa gcatgatatt ggcgatctgg ctgaacatgc ggatacgcat
     1801 ggacgcaatc tgaccgccct gggtcatatc ctttgcgtta aaactcatga tgaaactccg
     1861 ggaaaatctg gaacagggca gaaaaggatg atgtggggtt aattcacaga aacagaccag
     1921 gtgtaccctg tatgccggtg gtgatgcccg cctgcagtcc tgccggaaga gaccttctct
     1981 gtaacaatgt cagtcaggcg caatacttca gaccagtcac tcccatgcgg aaactggatc
     2041 tgaataaaaa ggcgtacccg ttcacggtga tccagaactg tgtccggttc ataaaaacgg
     2101 ccactgtcag gagaaacaca ggacgtttta ttgatgagat catacttatc ggcaggagac
     2161 tgcgatgaaa tcgcccaggt gatattctcc gcctggtggc catcaatacg actgctcccc
     2221 ggcgtccatg caatttcctg cgcactcgcc acgatgatct ccctggaaac ttcagccagc
     2281 tttctgggtg tacagaatac cggtgccagc acacacaggg acaacaaatt acagctgacc
     2341 gcctccggtt taagcaaatt gctcccggat ttttcaggaa aagatatttt tgtcagtatt
     2401 tcagttaatt ccttatgcct gagagaatta ataaagtaca tgataagcat catgagaaac
     2461 aatataacaa taagccctcc gataaccact aaaaggtcag tatcaatatt attgctgtac
     2521 agatagtctt tagtggtcac tgtattatcc tgtatcaccg gggggtattc agttgcaggc
     2581 ttctcagtaa attgattatt ctcaccaata tattcagcga tattgtctga tgtcataatg
     2641 actttctccg gaagatcccg gcttcgccgg caacacttca cggtcgcaca aagaatttgc
     2701 acaaccgctc agtgttttca tatttatttc ggaaacgccg ggaggtgaca aaatttattt
     2761 tgttacatcc cggggttgga caacgagcaa cgcgaggcgt cagcaaggag gccggtcaga
     2821 tcagcctcct gacgaggttc agagaatatt tgcgattgat ttggccagct ggtcttcgag
     2881 aacaggcttc gcctcttcaa atttcaggtt aaccttgttc gcatttgaga ccacacgggt
     2941 ctggtattta tgctggttac ctgtttcagt actggtctga attttcgcac cggatgtgcc
     3001 ctgacgcagg gcggcaacat tatccgttgt caccgttgcc ttagtacgct ctgcaatctg
     3061 cacatccgtg atcatggtat agttcacatc ttccaccatc gcatctgcag ccatacccac
     3121 cagaccagca gcaaggccca caccgagtgt ggcaccggca gaatttgagt tatagccggt
     3181 aataccggca cctaacgctg caccaacagc tgcgccttca taaccacggt tcagccatcc
     3241 ctgagactcc cgcagatcca tcttatcggc cttcagcaca ttcgcctgaa tccagtagta
     3301 ggctgcatcc ggagaagtca ccacctgata tccttttgct ttcacagcat cagcaatttt
     3361 gccctgcagc ccactcatgt ctttatcaga cgtgtttttg atctgcagaa ataccgtgcg
     3421 ttcgctggcg ggtttaagcc agatggtctc actcatctga gtcttcacct caaggttacg
     3481 cttcttgatt gctgtgctca tcgcaccaca ccctgaaagg gccagagtgg aactgaccag
     3541 tgcaaccatc atcatttttt ttgttttcat ccagaatatc cttttaacta acagactctt
     3601 ttttgaacag acctattact gagactattg cagagagatt ataacgattt atatctatag
     3661 tataaataaa ggaaataatt acaaacagag ttattgataa aaacccaaat aagaacgtat
     3721 gagcaaagga aactattgaa aaaacaatca caaacgacaa gatataagtt ttgttcttat
     3781 catacataaa agatataact ttcgactcat ctctgaattt tttagggatg gcaagataca
     3841 acatcattcc attagtaatg gcaacaaaaa ggataaaacc cattacgcca ctaaaacata
     3901 tatcaaatcc agcatctcgg atatcaactt tatctgccag caaacgaaga aatgtcggcc
     3961 ataataaaac ccataaaaac acatacaccc ctggttttat gacttgtaat aatggcggaa
     4021 tattaactcc ctctacagag agtacatcct ttagtttctg tgtctccgtt tttatatcat
     4081 agtgagtgat cataatggcc ccttcagtct atccttttta tcatcaacca tttttttcac
     4141 agcctcttct gatggctcag gtattatctt ttgactttct cgctcttcat tatatttatt
     4201 attctgagat aatgcctctg ttttatgatg attttgcagt tcagaatact gccctcttac
     4261 aatattttcc tcgccacgga tactgttctg agtatctccg atattgccac gatattctgt
     4321 gaccatatta tcaacctgat gttttacatc attacggata ttactgtcct gcgttctttg
     4381 ctcaataatg gcctgatgtc cctgatggtc agcaataata tcctgactgc caccacccga
     4441 aggtacgctc tccattcctt taccaatatc gccacgggat tcccgccagg cattatcaac
     4501 accaggctgt acctgttcct gcacaaaaaa ccaggccatc gcccgacgac gttctgcaat
     4561 ctccggcgaa ctggtatttg tcagaatagc ttctgcatcc tgcggtgcgc gtttcatcac
     4621 atactgtgca aactgttgcg acaggtcttc actcatctgc ccgctcatgc tttcagtacg
     4681 ggaggccatt tccgcatatt catggctgcg ggtcatattc gtcgtgtact ggtcgtaact
     4741 ctgtttcgct gagttcaggg ctgcagacaa ctggtccacc cgggagtcag cattattgtc
     4801 agtatggctg ccggactcac tgaccttgcg actggtaaag taatcgctgg cttctttaaa
     4861 gtccttactg gctttagcgt caatatcatg gcgagcatca tgggtagctc tgctgccact
     4921 acttgcagaa tgtgaatcat catccgttcg atcaagactt cccttaaacc cgcctctgac
     4981 accaacccca ccaccaacac caagaattcc aggtttaatc ccccattcag catgagcatc
     5041 tgcatatccg ccatacgaga acttagaacc tgttgatgcc agttcctgag tcgcctgctc
     5101 attactgata ttatgcgcct tcgcatagct ttccactgca ctgcgcatac ggctggccat
     5161 catggagtcc tgtgcactca tggtgctgtc agcgccaccg gtgacagaat cactgctccc
     5221 ccggttagaa ccaaactgac tgagcgtgtt ccaggcactg gcaatactgc tgctgaaacc
     5281 atgcagggca ctttctgctc tgttcgacgc ctcccgggcc atttcctgtt gcgccgctgc
     5341 aatctgacgc gttgcattga taccgaccgg taaacgggac atcgctccgc ttgcatccat
     5401 caccatatta ccgtcacggg tctgtgtggc agttgcgcca ctgccggtct ggtacatcat
     5461 ctgaccaaac gacgtggtgc tgttggtgct ccagctgaag ccgttcacgt tttccgtctg
     5521 catgttgccg taggagtaat taccgtcaac cacactgcct gctgcactgg ccgtcggact
     5581 gatagaggaa gaggcgaagt ggctgtacac gctggaaaaa cctgcgccaa ggccttttac
     5641 catcatccat gacaacgggg ggatcatggc tgaaagatac ccggcagtag aggccaggtc
     5701 agagtatttc agctgtatct gagagagttc agacagcacg accggcgcac cattcatctt
     5761 cgcatagaat gtcatggcac tgttcagaat ggcatacaac aacggccagg tctgcagcca
     5821 catcagggca aacacatagc ctttcaggac ggacagtgtc agtttgttga agacagctgc
     5881 cagtaccaga agcgggaaaa taccaatggc aattcccgtc aggatagtct gtaccattgg
     5941 caggtttcgc attgtcacat gaccgataga gacatgcgcc agacgttgct tctccatcga
     6001 tgacgtggtg gccagattca ccaggctggc ggtgtcacca ttacgggcag cattactggt
     6061 gataccctct ttcagggcat ttattgtgac gttctggcgc ataatctggc tggcagactg
     6121 actggagccg tagaaataac tgtaactgtc actgaccagt tgtctgaata acaggtccgg
     6181 gtccggtctg ccgccaaata tctgctgcac ataataatgc caggtcttgc ctcccgtctt
     6241 tgtatcgaga ttcagcctgt ctttcagcgt gaccgacgca tccttacagg ttatgaaatt
     6301 attattgttg tcataaacgc cccgtagcgg gctgggtcgg gaaaaaatca gcgtgtaggg
     6361 gtcagcggag gccatcagat cttccagcgt gtatttatga ttcaggtaaa tatcacccag
     6421 cacgcagttc tggacgtagt cctggaacag attgatgatt tccggattcc gggacaggaa
     6481 atcggtgctt ttcacaatca gattcgcccc gaacagcatg cctgtcttgc tgtaggtgac
     6541 gctgtccggt tgggtgaata tcatctcgta gctgaccacc atcgcatgcc cgatacgggt
     6601 cgtaagagaa agtggcatcg ccagaccgac cggaacatta tccacccgat ggactttgac
     6661 caggtcactg ttatcaataa tctgcaccga tgtgcggaca ttaaccagca ggctgataag
     6721 cacaaacacg gccacccagc ccagcaaatc catcacgtta tgccgctgta cccacataaa
     6781 agccacagca acaacagaca gggtgagcgc gattttttca attgaatccc aggtccgggt
     6841 actcataaat gcagcaatgg cgttcaggtt attccgcaac cactcaccac cggcaatcac
     6901 ataaacttca ttcacagtga attccctccg aagtgatagt tgttctggta acggctgagc
     6961 atacgggcgg aaagctgctg acgcatgtag ttcatctgac gttcgacaac cagcagcgca
     7021 tcctgctgga cctgtacctg actctggaaa atggcaatct gttgctgtgc ctgattcaga
     7081 ttctccagaa cattgtccat cacagcctgc ggatagttcc cggtggcaat catggcccgg
     7141 gcctgctgaa gcagttcctg aatgtactgg agcatgatgt cgtagccgat atagtccgtc
     7201 agctggtaaa cgacgctgtt ggacacaccg agcatctgcg ggtcaatcag atatttaaaa
     7261 acgggaatgg tggtgctgga aataaacccc tgttcccggg cgtcaagcgg agtgtcactc
     7321 actgctttat tctgaatact ggtgagcagt tttgtaatct gtgacttcag ggctttatcc
     7381 cgggcgatgg tcacatttgc atccgccacc accttcagac acttgtccga atcgttgcag
     7441 tggtaaattt ttgccgttcc cccctccata atcgcccgga ggatatcgcg gtcagtggta
     7501 cgcgccggca ggggggtaat ctcgctgtct tccccaaaaa tcagcgtacc ggtgagcgtc
     7561 atgataaatt ctttcaactc tttgttgccg tcaaacattc tgtttttttt cagcgcctcc
     7621 catatgatat tctggttttt cagcacccgc tccttgtcct tgtcgctggc tttgtcacga
     7681 acagatgagg actgccctcc gaccgtgcac ccctgacgag atgcggccca gtcggcgaag
     7741 atattgcttt cactgccaat gtcctgacaa actttctgct gagattcctg cgtctgaggg
     7801 aacagccccc ccacaagccc ctgtgccacc tgacaggaac tgaggttcat actgttaatg
     7861 tcacttgcca ttttctgcag gaagtctttt gcggttttta tttccggcac cgttgtctgc
     7921 agggcaaggt caaaaaagta accggcagca ttgctcataa tctgcttaac aaaacgctgc
     7981 aactgatcgc cattaataaa actgaacgag ccgagatagg cgtcgatgcc gccacatccg
     8041 gcattgatat ccggcagcgt catggaaatc agctggatat ttttgacctg ggtgcgggca
     8101 tacagggagc caccataggc atacccggcg gcctgcccct gccagacgcc cggctgtgtg
     8161 gtattggagg cgaagcccag cttgttaaag aactgattca tatcgctgtt cacatccgct
     8221 gacgctgcgg gtgtgaccgt cagtaaagcg gcacaaagaa ccagaagagg tttaatgcga
     8281 agcattattg ctgccctccc ttaatgtgtt ctcccggcgc atacggcgtt tcagcaggaa
     8341 tatccggaca cccccaaata ttatttcgca caacggcgac agagaagccg cagcaagaaa
     8401 accagctgca agcgcatata acataatcgg aatgacatac atcagggtgt tccacatata
     8461 acgaaaatta tttaattccg ggtcagcttt ccacgaatca ataccggcac tgaaaaaatc
     8521 atgcataacg ttccttacaa tcaatccggc agcaaagaaa accacaaaaa gccagaagta
     8581 tttttttgca ttatcacaac cacgttcaat ctccctttca atttgtttga ttcccggttc
     8641 tgtatcattt attttttctt tcatcataag ttccctctga tatttctgat gattcatgca
     8701 gacaggtcat gcggctgtaa tatatttcag acaccgaaat ccatcccaca attgccccca
     8761 caaaaaacac aacagaaaca gaaaaggtaa gacacagcga aacgacaatt tccacagggc
     8821 tggcgggata aaaaagcccc tgacggataa aggggatatt caggctgacg attattcccg
     8881 tcgcgatgcc ggatattccg gcaatgagta acaccacctg cttatttcgc acctttttcc
     8941 tctccgtata tctgcagaac ggtatccatc cgcgccataa aactggcggc atccgttgcc
     9001 ccctgcaaaa gcggtaatgc ctcaagtgtg ttgacgttga ccaggaaggt ggtcggtgtg
     9061 gccaccggga tattcgggaa gaaggtctgc atcacgtccg gtggcaccgg taatgcttcc
     9121 ggaaaggcag tatccccctg accatccagg gtgtagggaa aaacggaaaa gccgtactgc
     9181 tgtgccagct gcttcagtac cgggtcaaac tggtgacagt aagggcaatg cccctgcata
     9241 aacagcacca ccttccagtc ggccaggtta acctgcctgc cgttgctgag gcggaaccag
     9301 cggggagccg gcttttctgt tctggctgat gtgtctgctg ccggttgtgc aggctgcacg
     9361 gccattccct gcggattcca gagtcgttca atttcatccc gggtggaggc ctgcacacct
     9421 gtggccatca tcaacaaaat gaccggcagt aatttagtga gagacatgtc cgccctcctg
     9481 ctgcccttcc atcatggcct gttgcctggc ttccagtgcc tcacgctctt ccaggctgac
     9541 acgtgcaatc catgcatcca gcacctggta tgcgccgaac agcacaagga tggccgcaat
     9601 caactccgcc agaaaaaaag ccatccaggg tttgctgtaa acaagacggg cgacgatatg
     9661 aaaccagaca cccagggaaa atacccacat tcccgtaata tcaagccggg gtaaagagaa
     9721 tctgcgttta cttatcatgt taaactccgg ttattattta catgagagta ttcatgtctg
     9781 taatattcgc gtttgtgtga tattaatcag aggtacaaat gaaaaagtta tcttttaaag
     9841 aaaaactgga aatagtgaga aacatcataa gagagtcatt atcaggtaat gcggcttttg
     9901 ctttactggt gtattcatta ttgcactccc tccccattaa aacctttgct gatttatttg
     9961 tgacgatact gttatttata gcgattatcg ctgttgtgat atggctattt ctaatcatct
    10021 atatttattt ttgtgagtta ttcagaagtc actggatagc agtctggttt atcctctggt
    10081 ctgtggtaat caacctcact attggatttg gcatttatga caaattagca ccagtcattt
    10141 cactaatgaa ataataaaaa accacatcac tccagaccat atggctgaca acataataca
    10201 aacaaacatc cttctgaaac tgtcaaactt cgtgataacc ggtatggtac tgactgccat
    10261 tgaaacaacc ggtacggcac actgattcat aaccgcacca caaagaaaca agaaattatc
    10321 tggcagcata catacactta tgacatttac tacaaagaag acaaacgagc tcagcagata
    10381 aactccttta ttatttggtc ctgtaaagaa ataaccaaca acccccacga taaacaggct
    10441 ggcacctgta aaaaaacaca aatcccctgt atgcatatat aacctttggt taaaaattcg
    10501 gtttaaaatc ttcagaaacg ttcaggaact gttttgccag gtcgtcctgt gaaataaagc
    10561 cgtatgataa cgggcgaaca ctgccctgtt tcgggtcaac cagcatcatg gccgggaaat
    10621 atttcacacc gaggcgctgc gcctgcccct ggtcagtccg ggaatccggc aacagcggat
    10681 taatcacgcc atccacggaa acgggaataa cactcagacc atacgtatcc cggaagccat
    10741 taatgacctg cgccagttgc ccgtcgatgg ggtcctgccc ccggtaaaaa aacatgatgc
    10801 cgtagtgttc agccagtttc gcaatggcct gtcgctgctg cgcctggtct gccgccagct
    10861 ggttccgtac cgtgccgttg taatggctgt actgcaggtt atagtccagt tcgggatgtg
    10921 ccagcatggc ctttttagca ctcatggtga aaagcccggc ctgctgagtc cagtaattct
    10981 gcagccggaa atatttaaca aaattatcca cgccgggata cagaatggct tcgtacagcg
    11041 cccgctttgt tgccgtctgc agcgcggcca gtttctgcat aatatccggc tcctgacggg
    11101 gggctgcagg tacaggttta ttttcttttt ccttcggatt tattttctcg ttataccact
    11161 gccagcctgc atctttccct gacgccggaa aaataaagca gcagagtaac agtggcagta
    11221 atgctttatt catcttcatt cctttcatca ttttttcttt catcatcttt ctgcaggcca
    11281 gtcagggcag aaaaacaaaa atacagaata aacaggagca caccggagta aaggataacg
    11341 aaagcagcag caagtatccc gtctgccgta gtttctttta ccgtatccca cgaccagata
    11401 attaccggag aaatgacaat cgctgtaatc gaaagccgga taagaaaatc aacaagacga
    11461 ttagaagtga atataacttt catcatttcc cccggttatt ttccatacct tactgtcctg
    11521 cctgtttcag ctggtcagca atctgctctt tcactttctg cgtcagaacg ccactgtccg
    11581 ggattttctg gttattcatc aggtcttcgt agaagttagt gaaatccagt ctgtcgaact
    11641 gaattttctg cagctcatca accgtaatcc cccgacagtc aggatgcttc gcactgccaa
    11701 acccgatacg caactgcccg ttacgcccct gctgctggac tatctgcgcc agcttcgaat
    11761 caaactggca gtagctgcgc tttttctgca gacagacacc cagcactttc cgggagcaga
    11821 actcgcccac actcaccgtc agtttgttgt cctttgcctt tcccagcgcc ttttcttcac
    11881 tgttgcactt cgccagcccc acatcctgac cccagccgcc atccttacag cagttactga
    11941 agccggctgc ggccttacgg cagaatttcg cctcacccgt gaaagccctg acatccacac
    12001 cattcagggc ggcaacatcc ttacctgcgg cggcaagcgc agccagctgt gatactgctt
    12061 caccaaaatc actgtttttt ccgctctgtg ccttatcgca ttcaccgtca aggcagaaga
    12121 catcaccacc acagaccatc acttttcctg atgttctgga ctcgcaggag tacgtggcgt
    12181 actcatgcag acaggttccc tcatcggaat aaaacgcgca ctgacgtgag gccagcgtac
    12241 aggccggatt atcgacatat ttctgacagg tgccgttatc tgcggactga gtgacatatt
    12301 tatcccggta tgcccagcat gcctgtgtga tgttccaggg tttaccttcc atcactccgg
    12361 ttttcgtacc acccggctct gaacactcag tgcctgtcag cacgccatcc gctttattaa
    12421 acggacaact ctccacccac tccacccggg gaacccattc tttatcacgg accttcatcc
    12481 tgagtttcag cgtaaaggtg gaagcaccac tgacaagcga ttcatagact ttcctgtcac
    12541 cattcccgtg cgggaggcaa ttaccgttag cagtacagcc actaccggtc agaacctgcc
    12601 cctgtgtcac ctgcaaacct gtcgccccgg acagggggta agtatccgac tgcccgtttg
    12661 ccaggccgac attaaaggtt gtattcatga aggtataacg ggagttcagg aagaaaaaag
    12721 cggcgtatac agacaatgat gcactttcca ctgtaccggt taccggagcc gtgacactga
    12781 acaccagttt cagcccgttc atactgaacc ggaactgtga gtgcggtatc gtcacttcac
    12841 gatattcatc cgtgtatttc cagtccccgg tgatactggc agtccgtgtg cagtactcct
    12901 ccaccatcgt gtcccgctca caggtatagt ttgtgaattc actgcggttt atctcctgcg
    12961 cactgcactg ctgtccggta tttcccacaa tgctgtcagc cctgttcacc acatccctgc
    13021 cggtctgaat gaacggtgca tccggtgaca gaatgtcttt cggcttgttc atgaaggact
    13081 ctgtgatggt ttttccggtt tcaccggtcg cccattccgt ggtgccgtca tttttcaggc
    13141 cgccatcccc gccggcagtc acgccaccgt aatattttgt ctcgtccgga ttcgcgttat
    13201 agccggggat actctcctgt ggcttaaagc cctgaatact gctgcttccc tgccctttga
    13261 tctggtgagc aaaatcagaa ccggcccggt aatcactgtt gctgtcagca aacacagaaa
    13321 agctcagaaa aaaaacaatt gcacaaagat tgcacggctt tacggaataa ttaaaattca
    13381 gagatctgaa aaaggtattt atcttttctt tcataacctg aaccatactg gagaatgaag
    13441 tgatgtcatc tcaacttcag aactgtgttg attgtggtaa agcattaagt cgaacagcca
    13501 caacgtgcgg gaattgcagc tcaacggacc cttttggaaa aaaacggcgc agcgatcgct
    13561 tttaccagtg gtttgtaggg ctgtttatta cggctattat tattgctggc ggattatggt
    13621 acatggggct tcttgacgta ccgggattac tactcagata acgacttcag taacgatatc
    13681 agtgttctga aatcggcaca taacgagtga cgacaatacc ggttatacca gtaatgaggg
    13741 gtgtcgggct gaagcctgaa acagagctga aacttactgt taaaagcccc cttcaccgac
    13801 catccccatg ccagacgtgg tattgctgat tcagcatcct gccgcagtcg ctgctcaaga
    13861 agggcagcca tttttaccgg acaggcaaag gcaatataag gaggcagctt tgtaacaggg
    13921 aaaccatgtc cctgacagga agcatatgtg cggaagccgt gaatgttcat cgcggccacc
    13981 agggcacgga tctccggatc aatctgataa tacccggcag gtaaagtcat ttttgtttat
    14041 ctcccgggtt attcagcaac gcccgagcca cctgccggca gtcccccgtg gcggccactt
    14101 tctccagcgc ctggccgacc cgcaggttac ctcggataat gtcgtatccc tgactgcaga
    14161 acaccaccag tgccggtaca ctgcgaatgc cgtactgtga aaacagcgtc gggtcgatct
    14221 gaacaccatc agtcgcgccg tctttcacca gggacagcac ggcttcagcg gtggtcttca
    14281 ggtcattatt caccatgccc cgcagtgtgg cgggtatacc gtagtgccgg gtttcaccca
    14341 gcatccgttt cagcccctct tcgggaatgg aaaacgacac aaaatacagg gcaccctgcc
    14401 ggggtttccc gtcctgcgaa gcctgctgtt tacgaaccag ctcatccaga aaatggttat
    14461 ctgaacgctg aagggggttt tccagcacct gtttctccgc ccaggctttc agctgatggt
    14521 caggtttctc tcgcagttgt ctgcttaaat tttcctgctg cttcaggaac tggcggtttt
    14581 caggtgtgtt cacattttct gaagcatgaa cagcaagatg catccctgct gtcagagcaa
    14641 aaagtgcaaa tattttccgg ttcatgatat ttctcacttt ctgctattta caggcatgaa
    14701 ctcatcagaa aaaagataat tgagaaaaac agccataaac gcacatgtaa atgtcacctt
    14761 cagatataat ctgacaagtg tcacttgttc tgatatatct gccttcttcc attcatgaat
    14821 aatttcagcg agaaaactgt caggtgcaaa ataaaataat ataacgggca ccattacaag
    14881 accaaaagga aataatttta atgttctgct tatcagcctg agcagcacag cacttatttt
    14941 cctgaggtgc tgaatatatt tactgtccat tgttgccatt ttcatttcct cttattcatt
    15001 aaaatgattt acaggaatac acagttacgt ttacgccaca tcaggtagcc gaagtttttc
    15061 tttgtgttgg gcggattttt tcccgtctcc cagcgcatca cactgcgccc gaacggatgg
    15121 cactgcccgc tgtccggata catattcacc atctgataac gccagcgttc tttgggcagg
    15181 attggggacg gatattcatt acagacggcg ttatttttcc cgatggtttc cataatcatg
    15241 ccctgacggt gtagtttgaa cgccatgcgt tcactgacca gcagggagga ctgcaacgga
    15301 ctggactcat tactcaccca gccattgaac gggtacatac ttccctgcga accggcacac
    15361 cagaacagaa catcgagagg catattaaag gcgctggcaa tggcatctgc tgcacaggcc
    15421 ccctgtgcta tcggattggc aaagatgaca gcttccggat tgagaatggt ggtcaggctg
    15481 ctgtccgtcc aggtggggtc aatttcagaa agataagcga tatccaggtc accaccttcc
    15541 agacagccca gcgatgtgat gatgttcagc cagtacgtca gcgggtattt gtaccagtga
    15601 acgtgataga acgccccgtt cacctgcttc tcatcctttt tcgccgttcc ctgtgccgtt
    15661 ttaccaaaag ccggcaggct gaagcccagg ttaaccatgc atcccggtga ccgggtgacg
    15721 tccgtcagcg ccatcggctc ccagtaacca atggccagcc cgatacgcct gaacagcggc
    15781 ggcggtgccg gacaaatctg aatgggcatc gacgggttcg ccgtgtcggg gaccttaccc
    15841 tgactgactt tgacactgcc cagcgagagc gggaaaatac agctccagca gatatccgtg
    15901 atcgggttta caaaacgccc ctcacaggca gaatccgcag acgctgcagg gacatgaccg
    15961 gcgagaagga ataacatcag cagccacagg cttcgcttca ttttctgccc tcctctgccg
    16021 gaataaattc caccttcagg aagcgatcgc cgggaacggc gctcacccgt gccgggacct
    16081 gatcaatgcc gaggcgctgg cagagcacac cgttctggtc aaagtaaaca cggctgtcca
    16141 gagacttctg catctccggg atactgccct gcacgaggat aattttgctc tccagtgtgg
    16201 gcggcgtctg gcgtttcatc caggccacct gcgccggatc atcgccattg atgaaataca
    16261 gcgtctggtt aaaaggcaca tactgcagcg gattcatcac ttcaccctga cgggcaaaca
    16321 cccgcccttc gttatcccgg atatccgcag ccagtctgac ggacgggtca aacaaccggc
    16381 tgccgtattt ctccgtgcgt ccgataccgg gcacggcagg gggccgcaga ctgttgcgga
    16441 tcacccgctc cttaaaggca tccattttcc ggcccatctc accggactgc tccagtgccg
    16501 tcaggcgctg catgatcacc gtcagcatgt ccggctcctt taccggccac agatcgcccc
    16561 aggtgccaag atcggcggcg gccacactct gcccccatat cagcagggca atcagccccc
    16621 ggcatctcat ggttccgctc tcattcgccg ggcaatatcc tgctggattt cccgggtgat
    16681 atccggagcc ccctgtacca cggcaggcga caccagaatg accgcgtggt gtttctgctg
    16741 ccacgcctgc aggctggctt ccagtgccgt gttaaaacgc gctgaaaggg cttttgactg
    16801 tgcttctgac agttgtttct gactggcgct gtcaaaaaat gcatccacag tctgtttcat
    16861 gttgaaggcg gcagtcaccg gtgcgttcag tctgacaatg ccgtaactga cggctgcgtt
    16921 cagtaacacc attgccaggc atcccggcac cgtccaccac cagtggctgc gtcggggggc
    16981 tgtaacatca gtcgtttttt gcgttgtggt catgcaacac tcctgtattt ctcatgttct
    17041 tccagccagg cttccagcga agccatctcc ggccctgact tcttccacgc cagctgccac
    17101 accgcctcat gaatactcag gccctccctg cgtttctgct gcacaaactc aaaatccggg
    17161 ccgtcagagc tgtacatggc gcggcttaac gggtccacaa acagacggtg ccaggaggaa
    17221 tggttttcca cctgcagcag gaaggaactg aaccactggt ctttggccgc accaaacttg
    17281 ccaatcatgt cgcgctgcag tggctggaac tgatccggga ataactggtt atatttcgcg
    17341 aactccttcg cactctgttt gaggataatt ttgtaggagg agttacccca tgccgcacgg
    17401 gccgcactgg aggccttgtc agagtcaaag tcgacgatgt tctgtgtgat ggtgatatag
    17461 gcaccggtat ggcggcgaca ggtgcggtaa cctttttgaa taaactcacc gaccttgcga
    17521 tttttgaagt ccagcagacg ccagccttca tcaatgacgt tcagtttctt gagagtacgc
    17581 ggcgtgcggt acatcctgtt ctcgatgtag ataatcaggg agaacatcac cgcaaccagc
    17641 agtgacggac ggtcttccag tccgcccagc tccagcacca ccattttcgc atcatcccgc
    17701 agggacggct catcggagtt aaaatactgg ccgtaagtgc cgttggcagt gtactggtca
    17761 agcagcacaa tcatttcgtc cagacggctg cggatggtcg gtgacccggc atactgctcg
    17821 ctgtcactgg cgtttttcag gaaatccacc acgtcatcaa tacgtgccct gttctctttg
    17881 gccagccagg aggccctgac cgcctgcagc agcagacctt catgcacttc atccaggtta
    17941 ccgttggggc tggccatcac tgacaactgg tcacggacac gctccgctga ctggtcaata
    18001 tcggtgatgt tcgcaaacgg gttaaaacgc agggtttcac cgtccagata tacgcccccc
    18061 atgttctcac acagggactt gtagccatcc cccatgtcga acaccacggc aaagcccccg
    18121 gagtccagca cgctgcggat aagtggctgt atcagtccgg ttttaccggc cccggaggtg
    18181 ccacagaccg ccatgttgta gttggtgtta ttcatcccct taaagaagat atcgataaac
    18241 gccagctggt tacggtaggt gggcgccagc agacctgccg gtgtcagggg gttatccgcc
    18301 actaacggca tcaggttggc cacattaaag ctctctgcgc gctgaaccac accggcctct
    18361 ttcagctgtt taaacagccc tttcccggcc ataaagggca ggcaggtcag gaaattgcgc
    18421 atgtggttaa agcgcggtga aatcagctca aaaccgtttt tacggaagct gttcaggatg
    18481 tcctgctcca cttccagtgc cgtttcatta ttgtccttgc agaaggctgt gatgttgagg
    18541 aagtaggaca cgacagaaga ctggccggag cccagccgct ggcgcagttc cccccactcc
    18601 ttcgcctctt tctccacgga cggaaaccat ttggcatatg aggtcttcga cttcttctcc
    18661 aggtccatgt acttcaggtt ggcttcgctg tgggttttca cctggtcttc caccaccaga
    18721 gtcagcgtca ggatga
//
LOCUS       DAEBXN010000082        18450 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.082, whole genome shotgun sequence.
ACCESSION   DAEBXN010000082 DAEBXN010000000
VERSION     DAEBXN010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18450
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.082"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            1..1238
                     /locus_tag="KJL99_002886"
                     /pseudo
     CDS             1..1238
                     /locus_tag="KJL99_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
     gene            1388..1819
                     /gene="ndk"
                     /locus_tag="KJL99_002887"
     CDS             1388..1819
                     /gene="ndk"
                     /locus_tag="KJL99_002887"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HBD4437547.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            1969..3123
                     /gene="trmG/rlmN"
                     /locus_tag="KJL99_002888"
     CDS             1969..3123
                     /gene="trmG/rlmN"
                     /locus_tag="KJL99_002888"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HBD4437548.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPDAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            3408..4421
                     /gene="rodZ"
                     /locus_tag="KJL99_002889"
     CDS             3408..4421
                     /gene="rodZ"
                     /locus_tag="KJL99_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HBD4437549.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            4448..5575
                     /gene="ispG"
                     /locus_tag="KJL99_002890"
                     /gene_synonym="gcpE"
     CDS             4448..5575
                     /gene="ispG"
                     /locus_tag="KJL99_002890"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HBD4437550.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVGKIIT"
     gene            5678..6952
                     /gene="hisS"
                     /locus_tag="KJL99_002891"
     CDS             5678..6952
                     /gene="hisS"
                     /locus_tag="KJL99_002891"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HBD4437551.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELLGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            6970..7590
                     /gene="yfgM"
                     /locus_tag="KJL99_002892"
     CDS             6970..7590
                     /gene="yfgM"
                     /locus_tag="KJL99_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HBD4437552.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGGILGVGALTGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            7601..8779
                     /gene="bamB"
                     /locus_tag="KJL99_002893"
     CDS             7601..8779
                     /gene="bamB"
                     /locus_tag="KJL99_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HBD4437553.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            8897..10369
                     /gene="der"
                     /locus_tag="KJL99_002894"
     CDS             8897..10369
                     /gene="der"
                     /locus_tag="KJL99_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HBD4437554.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVVNKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            10439..10654
                     /locus_tag="KJL99_002895"
     CDS             10439..10654
                     /locus_tag="KJL99_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBD4437555.1"
                     /translation="MELHCPKCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(10651..12021)
                     /gene="xseA"
                     /locus_tag="KJL99_002896"
     CDS             complement(10651..12021)
                     /gene="xseA"
                     /locus_tag="KJL99_002896"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HBD4437556.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAECLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTSVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKTGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            12183..13649
                     /gene="guaB"
                     /locus_tag="KJL99_002897"
     CDS             12183..13649
                     /gene="guaB"
                     /locus_tag="KJL99_002897"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HBD4437557.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            13718..15295
                     /gene="guaA"
                     /locus_tag="KJL99_002898"
     CDS             13718..15295
                     /gene="guaA"
                     /locus_tag="KJL99_002898"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBD4437558.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPIFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(15390..15929)
                     /locus_tag="KJL99_002899"
     CDS             complement(15390..15929)
                     /locus_tag="KJL99_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HBD4437559.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(15945..16463)
                     /locus_tag="KJL99_002900"
     CDS             complement(15945..16463)
                     /locus_tag="KJL99_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HBD4437560.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQEGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            complement(16774..16965)
                     /locus_tag="KJL99_002901"
     CDS             complement(16774..16965)
                     /locus_tag="KJL99_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HBD4437561.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            16983..17135
                     /locus_tag="KJL99_002902"
     CDS             16983..17135
                     /locus_tag="KJL99_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4437562.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            17317..>18450
                     /gene="pdeF"
                     /locus_tag="KJL99_002903"
     CDS             17317..>18450
                     /gene="pdeF"
                     /locus_tag="KJL99_002903"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HBD4437563.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQ"
BASE COUNT         4361 a         4481 c         4992 g         4616 t
ORIGIN      
        1 ggcacgttac cttgaagcgc tgatcaatta tgaagatcgc tggttctgga agcatccggg
       61 ggttaatcca ttctcggtgg cgcgcgcagc atggcaagat ctcacttcgg gacgggttat
      121 ttccggtggc agcacgctca ctatgcaggt tgctcgtctg cttgatcctc accccaaaac
      181 atttggtggc aaaattctcc agctctggcg cgcgttgcaa ctggagtggc atttgtctaa
      241 gcgtgaaatt ctgatcttgt atcttaaccg cgctccgttt ggcggtacgt tgcaggggat
      301 cggtgcggca agttgggctt atctcggaaa atcgcctgcg aatttaagct attccgaggc
      361 ggcaatgctg gcggtgttac cgcaagtgcc cagccgactg cgcccggatc gttggccgga
      421 gcgtgccgaa gccgcgcgtc gccccactgg catggaaaac gggcaccagc tatggctatc
      481 gtgacgcctg ggcgattggg gttaacgctc gctatgtcat tgggatctgg actggcagac
      541 ctgacggcac gcccgttgtt ggtcagtttg gctttgccag tgccgtacca ttgttgaatc
      601 aggtcaataa tatcttactg tcgcgcagtg cgaatctgcc ggaagacccg cgcccgaact
      661 cagtcactcg tggcgttatc tgctggccgg gcggacagtc tttgccggaa ggtgacggta
      721 actgtcgccg ccgcctggca acctggctgc tggatggcag ccagccgcca acgctgttac
      781 tgtcagagca ggaagggatt aatggcattc gcttccccat ctggctggat gacaaagggc
      841 agcgcgtcgc cgctgactgc ccgcaggcga agcaagaaat ggtaaatgtc tggccgctac
      901 cgctggaacc ctggctgccc gcatcagaac gtcgcgctgt gcgcttacca ccggcctcga
      961 caatttgccc gccatacggt cacgatgccc agctccctct gcagctgaca ggcgttcgcg
     1021 atggcgcgat tattaaacgt ttaccgggcg cagcggaagc gactttgccg ttgcaatcaa
     1081 gtggaggggc aggtgaacgc tggtggtttc tgaatggcga accgttaact gaacgcgggc
     1141 gcaacgtgac cctgcatttg acggataaag gtgattatca attgctggtg atggatgatg
     1201 tggggcaaat cgcgacagtg aaatttgtca tgcaatagtc tgttttgtcc ctgattgttg
     1261 ctaaaactca ttttatttta aaaaaaatgt taccttcatc aatagtcaac ggccctgttg
     1321 ctcattataa tccgcgccat ctcgtacgct ggtacagaca acaacagaac aatttacaga
     1381 ggtaaaaatg gctattgaac gtactttttc catcatcaaa ccgaacgcgg tagcaaaaaa
     1441 cgtcattggt aatatctttg cgcgctttga agctgcaggg ttcaaaattg tcggcaccaa
     1501 aatgctgcat ctgaccgttg aacaggcacg tggcttttat gctgaacacg atggaaaacc
     1561 gttctttgat ggtctggttg aattcatgac ctctggcccg atcgtggttt ccgtgctgga
     1621 aggtgaaaac gccgttcagc gtcaccgcga tctgctgggc gcgaccaatc cggcaaacgc
     1681 actggctggt actctgcgcg ctgattacgc tgacagcctg accgaaaacg gtacccacgg
     1741 ttctgattcc gtcgaatctg ccgctcgcga aatcgcttat ttcttcggcg aaggcgaagt
     1801 gtgcccgcgc acccgttaat aatttcgtaa atgccgcgtg caaacgtggc atccgtgcgc
     1861 cagaatttgt acaatgcagc gcccccggac gagcagccgc tcaccggggc gtttcttttt
     1921 tcaaccctcc aggggccata acgtgtaata acgaggccgg aatagcatat gtctgaacaa
     1981 ttagtcacac ctgaaaacgt caccacgaaa gatggaaaaa tcaacctgct ggatctcaac
     2041 cgtcagcaga tgcgggagtt ttttaaagat ttaggtgaaa aaaccttccg cgccgatcag
     2101 gtgatgaagt ggatgtatca ctattgctgc gacaactttg atgagatgac cgacatcaac
     2161 aaagtgttgc gcggcaaact gaaagaggtg gcggaaatcc gcgcaccgga agtggttgaa
     2221 gaacagcgtt catccgacgg caccattaaa tgggcgatcg ccgttggcga tcagcgcgtc
     2281 gaaacggtgt atatcccgga agacgaccgt gccacgctct gcgtctcttc gcaggtgggg
     2341 tgtgcgctgg agtgtaaatt ctgttccacc gcccagcagg gctttaaccg caacctgcgg
     2401 gtatcggaaa ttattggcca ggtgtggcgt gcggcgaaaa tcgtcggcgc agcaaaagtc
     2461 accggtcagc gcccaatcac taacgtcgtg atgatgggca tgggcgagcc gctgctcaac
     2521 ctgaacaacg tcgttccggc gatggaaatc atgcttgatg atttcggttt tggcctgtct
     2581 aaacgtcgcg tgacgctttc cacttccggt gtagttccgg cgctggataa actgggcgat
     2641 atgatcgacg ttgcactggc aatttccctg cacgcgccga acgacgaaat tcgtgacgaa
     2701 attgtgccga tcaacaaaaa gtacaatatc gaaacgttcc ttgctgcggt gcgccgttat
     2761 ctggagaaat ccaacgccaa tcagggccga gtcactattg aatacgtgat gcttgaccac
     2821 gttaacgacg gcactgaaca cgcgcaccaa ctggcggaac tgctgaaaga tacgccgtgt
     2881 aagatcaacc tgatcccgtg gaacccgttc ccggacgcgc cgtatggacg cagttcgaac
     2941 agccgtatcg accgtttctc aaaagtgctg atgagctacg gtttcaccac tattgtgcgt
     3001 aaaacgcgtg gtgatgatat cgatgctgcc tgtggtcagt tggcgggcga tgttatcgac
     3061 cgtacgaaac gtactctgcg taaacggatg cagggtgaag ccatcgatat taaagcggtc
     3121 tgatagccat tacgccacgg ttactttatg taccgtggcg taatctttta tgaatgaagt
     3181 attaagttgc aattgtctgg tcaatattaa cggtgcgttt ttgctgactt taaggcagta
     3241 tgtaacgatg caacagtaag ttagcctcag tgaatgtggg ctttgtcacg agcacacaga
     3301 cggtcttata ctgtatgata acggttaact taacggatgt ttcgcggtgt gggtgagcat
     3361 gatggttcac cggcatctca attctcattt aaacgtacct gcagcgaatg aatactgaag
     3421 ccacgcacga ccaaaatgaa gcacttacta ccggcgctcg cctgcgtaat gctcgcgaac
     3481 aactaggact tagtcagcag gccgttgccg agcgactttg cctgaaggtt tccacggtac
     3541 gcgacattga agaagataag gcacccgccg atcttgcttc aacattcctg cgcggatata
     3601 tccgctctta tgcgcgtctg gtacatattc cagaagaaga actgctgcca gggctggaaa
     3661 agcaggctcc acttcgggct gcaaaagttg cgccaatgca gagtttttcc ctcggtaaac
     3721 gccgcaaaaa acgcgacggc tggctaatga ccttcacctg gctggtgttg tttgtggtta
     3781 tcggcctgag cggtgcctgg tggtggcaag accacaaagc tcagcaggaa gagatcacca
     3841 ctatggccga tcaatcttcg gcggaactga gcagtaatag cgagcagggg cagagtgttc
     3901 cgttaaatac gtcgacaact acagacccgg ctacaacgtc cacgccgcca gcgtctgtgg
     3961 atactaccgc aaccaacacg caaacacctg ccgtaactgc gccagcacca gctgttgatc
     4021 cgcaacagaa tgcggttgtt tcgccttcgc aggcaaatgt tgataccgcc gcgaccccgg
     4081 taccaacggc agcaacaacg ccagatggtg ctgcgccgtt gccaaccgat caggctggcg
     4141 tgaccacgcc ggcggctgat ccgaatgcgc tggtgatgaa ctttactgcc gattgctggc
     4201 tggaggtcac tgatgctacc ggtaaaaaat tgtttagcgg gatgcagcgt aaagacggta
     4261 atttgaattt aaccggccag gcaccgtata aactgaaaat tggtgcgcca gccgcagtac
     4321 agatccagta tcaagggaaa cctgtcgatc tgagtcgttt tatcagaact aaccaggttg
     4381 cgcgtctgac cctcaatgcc gaacaatcac cggcgcagta acagacgggt aacgcgggag
     4441 atttttcatg cataaccagg ctccaattca acgtagaaaa tcaacacgta tttacgttgg
     4501 gaatgtgccg attggcgatg gtgctcccat cgccgtacag tccatgacca atacgcgtac
     4561 gacagatgtc gaagcaacgg tcaatcaaat caaggcgctg gaacgcgttg gcgctgatat
     4621 cgtccgtgtc tccgtaccga cgatggacgc ggcagaagcg ttcaaactca tcaaacagcg
     4681 ggttaacgtg ccgctggtgg ctgacatcca cttcgactat cgcattgcgc tgaaagtagc
     4741 ggaatacggc gtcgattgtc tgcgtattaa ccctggtaat atcggtaatg aagagcgtat
     4801 tcgcatggtg gttgactgtg cgcgcgataa aaacattccg atccgtattg gcgttaacgc
     4861 cggatcgctg gaaaaagatc tgcaagaaaa gtatggcgaa ccgacgccgc aggcgttgct
     4921 ggaatccgcc atgcgtcatg ttgatcatct cgatcgcctg aacttcgatc agttcaaagt
     4981 cagcgtgaaa gcgtctgacg tcttcctcgc tgttgagtct tatcgtttgc tggcaaaaca
     5041 gatcgatcag ccgctgcatc tggggatcac cgaagccggt ggtgcacgca gcggggcagt
     5101 aaaatccgcc attggtttag gtctgctgct gtcggaaggc atcggcgaca cgctgcgtgt
     5161 atcgctggcg gccgatccgg tcgaagagat caaagtcggt ttcgatattt tgaaatcgct
     5221 gcgtatccgt tcgcgaggga ttaacttcat cgcctgcccg acctgttcgc gtcaggaatt
     5281 tgatgttatc ggtacggtta acgcgctgga gcaacgcctg gaagatatca tcactccgat
     5341 ggacgtttcg attatcggct gcgtggtgaa tggcccaggt gaggcgctgg tttctacact
     5401 cggcgtcacc ggcggcaaca agaaaagcgg cctctatgaa gatggcgtgc gcaaagaccg
     5461 tctggacaac aacgatatga tcgaccagct ggaagcacgc attcgtgcga aagccagtca
     5521 gctggacgaa gcgcgtcgaa ttgacgttca gcaggttgga aaaataataa cgtgatggga
     5581 agcgcctcgc ttcccgtgta tgattgaacc cgcatggctc ccgaaacatt gagggaagcg
     5641 ttgagggttc atttttatat tcagaaagag aataaacgtg gcaaaaaaca ttcaagccat
     5701 tcgcggcatg aacgattacc tgcctggcga aacggccatc tggcagcgca ttgaaggcac
     5761 actgaaaaac gtgctcggca gctacggtta cagtgaaatc cgcttgccga ttgtagagca
     5821 gaccccgcta ttcaaacgtg cgattggtga agtcaccgac gtggttgaaa aagagatgta
     5881 cacctttgag gatcgcaatg gcgacagcct gactctgcgc cctgaaggga cggcgggctg
     5941 tgtacgcgcc ggcatcgagc atggtcttct gtacaatcag gaacagcgtc tgtggtatat
     6001 cgggccgatg ttccgtcacg agcgtccgca gaaagggcgt tatcgtcagt tccatcagtt
     6061 gggctgcgaa gttttcggtc tgcaaggtcc ggatatcgac gctgaactga ttatgctcac
     6121 tgcccgctgg tggcgtgcgc tgggtatctt cgaacacgta actcttgagc tgaattctat
     6181 cggttcgctg gaagcacgcg ccaattaccg cgatgcgctg gtggcattcc ttgagcagca
     6241 taaagaaaag ctggacgaag actgcaaacg ccgcatgtac actaacccgc tgcgcgtgct
     6301 ggattcaaaa aatccggaag tgcaggcgct tctcaacgac gctccggcat taggtgatta
     6361 tctggacgag gaatctcgtg agcattttgc cggtctgtgc aaactgctgg agagcgcggg
     6421 gatcgcttac accgtaaacc agcgtctggt gcgtggtctg gattactata accgtaccgt
     6481 tttcgagtgg gtgactaaca gtctcggctc ccagggcacc gtgtgtgcag gcggtcgtta
     6541 tgacggtctt gtggaacaac tgggcggtcg tgcaacaccg gctgtcggtt ttgcgatggg
     6601 cctcgaacgt cttgtattgt tagtacaggc cgttaatccg gaatttaaag ccgatcctgt
     6661 tgtcgatata tacctggtgg cttcaggtgc tgatacacaa tctgcggcta tggcattagc
     6721 tgagcgtctg cgtgatgaat tactgggcgt gaaattgatg accaaccacg gcggcggcaa
     6781 ctttaagaaa cagtttgccc gtgctgataa atggggtgcc cgcgttgctg tggtgctggg
     6841 tgagtctgaa gtggctaacg gcacagcagt agtgaaggat ttgcgctctg gtgagcaaac
     6901 ggcagttgcg caggatagcg tagccgcgca tttgcgcacg ttactgggtt aaggaaggag
     6961 aaggacagcg tggaaattta cgagaacgaa aacgaccagg tagaagcggt taaacgcttt
     7021 tttgctgaaa atggcaaagc actggctgtt ggggggattt tgggcgttgg cgcactgact
     7081 ggctggcgct actggaacag ccatcaggtt gattctgcac gctccgcttc tcttgcctat
     7141 caaaatgcgg ttaccgcagt gagcgaaggc aaaccggata gcatcccggc ggcggaaaaa
     7201 tttgctgctg aaaataaaaa tacttatggt gcgctggctt ctttggaact tgcgcagcaa
     7261 tttgttgaca aaaatgaact agagaaagct gccgcccagt tacaacaggg gctggcagac
     7321 acgagcgatg aaaatctcaa agccgtgata aatctgcgtc ttgctcgcgt tcaggtacag
     7381 ctcaagcagg ctgatgccgc gctgaaaacc cttgatacca tcaaaggtga agggtgggct
     7441 gccattgttg ccgacttgcg tggtgaagca ttgctgagca aaggtgataa gcaaggtgcg
     7501 cgtagtgcat gggaagcagg cgtgaaaagc gatgttactc cggcactgag cgaaatgatg
     7561 cagatgaaaa ttaataattt gtccatctga gagggacccg atgcaattgc gtaaattact
     7621 gctgccagga ctgctttccg ttaccctttt aagcggctgt tcgctgttta acagcgaaga
     7681 agatgtggta aagatgtccc cattgccaac cgttgaaaac cagtttacgc cgaccacggc
     7741 gtggagcact tccgttggta gcggcattgg caacttctat tccaatcttc atccggcact
     7801 ggcggacaac gttgtctatg cagcggaccg cgctggtttg gtaaaagcgc tgaatgcgga
     7861 tgatggcaaa gaaatctggt ctgtcagcct ggccgagaaa gatggctggt tctctaaaga
     7921 gcctgcatta ctttctggcg gtgtgaccgt gtctggtggg catgtctaca ttggcagcga
     7981 aaaggcgcag gtttacgcgc tgaataccag cgatggtact gtggcatggc aaactaaagt
     8041 cgcgggtgaa gcactttcgc gcccggtggt cagcgacggt ctggtgttaa tccacaccag
     8101 taacggtcag ttacaagcgc tgaacgaagc tgacggcgct gtcaaatgga cagttaacct
     8161 cgatatgcct tcgctctctt tgcgtggcga gtctgcgccg gcaacggctt ttggtgcggc
     8221 cgtcgtgggt ggcgataatg gtcgcgtcag cgcagtgctg atggaacagg gccagatgat
     8281 ttggcagcag cgtatttccc aggcgaccgg ttctaccgaa attgaccgtc tgagcgatgt
     8341 tgacacgact cccgtcgttg ttaacggcgt tgttttcgcg ctggcctata atggtaacct
     8401 gacggcgctt gatctgcgca gtggtcagat tatgtggaaa cgcgaactgg gttcggtgaa
     8461 tgatttcatc gtcgatggca atcgcatcta tctggtcgat caaaatgacc gggtgatggc
     8521 gttgaccatt gatggcggcg ttacgctgtg ggcacaaagc gatctgctgc atcgcctgct
     8581 gacttctccg gtgctgtata atggcaacct ggtggtcggt gacagtgaag gttatctgca
     8641 ctggattaac gtcgaagatg gtcgtttcgt tgcccagcaa aaagttgata gttccggttt
     8701 ccagactgaa ccggttgccg ctgacggcaa actgctgatc caggcaaaag acggaaccgt
     8761 gtactctatt acacgttaat cgtctctgtc gttcactttg aaaacggctc ctggacaggg
     8821 gccgttttcc tgtttttaac aacgacgcga atatagcgtg cgttgtctga tgatttataa
     8881 aaatgaggct ttaaacatgg tacctgtggt cgcgcttgtc gggcgcccta acgtaggaaa
     8941 atccacgtta tttaaccgtc taactcgcac ccgagatgcg ctggttgcgg atttcccggg
     9001 tctgactcgt gaccgtaagt acggtcgtgc ggaaattgaa ggccgtgagt ttatctgtat
     9061 tgataccggc gggattgatg gcacagaaga cggtgtagaa acccgcatgg cggaacagtc
     9121 gctgctggcg attgaagaag cggacgtcgt actgtttatg gtggatgcgc gcgcgggcct
     9181 gatgccggca gatgaagcga ttgccaaaca tctgcgctcc cgtgaaaaac cgaccttcct
     9241 ggtggtaaac aaaactgacg gtctggatcc cgatcaggca gtggttgatt tctactcgct
     9301 tggtttaggt gaaatctacc cgatcgccgc gtctcacggt cgtggcgtat taagtctgct
     9361 ggagcatgtg ctgctgccgt ggatggaaga tctcgcaccg caagaggaag tcgacgaaga
     9421 cgctgaatac tgggcgcaat ttgaagcgga agagaacggc gaagaagaag aggaagacga
     9481 cttcgacccg caaagtctgc cgatcaaact ggcgattgtg ggtcgtccga acgtaggtaa
     9541 gtctacactc actaaccgta ttcttggaga agagcgcgtt gttgtttacg acatgcctgg
     9601 cacgacgcgt gacagcatct acatcccaat ggaacgcgat ggacgtgagt atgtgctcat
     9661 tgacaccgct ggcgtacgta aacgcggcaa aatcaccgat gctgtagaga aattctccgt
     9721 aatcaaaacg ttgcaggcca ttgaagacgc caacgtggtg atgttagtga ttgatgcgcg
     9781 cgaaggtatt tccgatcagg atctctcgct gctgggcttt attctcaata gtgggcgctc
     9841 acttgtcatt gtggtgaata agtgggatgg cctgagccag gaagtgaaag agcaggtgaa
     9901 agaaacgctg gacttccgtc tgggctttat cgattttgct cgtgtgcact ttatctctgc
     9961 cttgcacggc agtggtgttg gtaacttgtt tgaatcagta cgtgaagcgt atgacagctc
    10021 cacccgtcgt gtggggacct ctatgctgac gcgcatcatg acgatggctg ttgaagatca
    10081 ccaaccgccg ctggtacgcg gtcgtcgtgt gaagctgaaa tatgcccacg ctggtggtta
    10141 taacccgccg attgtggtga ttcacggtaa tcaggtgaaa gacctgcctg attcctacaa
    10201 gcgctacttg atgaactact tccgcaaatc gctggacgta atgggatcgc cgattcgtat
    10261 tcagttcaaa gaaggggaaa acccgtatgc gaacaagcgt aacaccctga cgccaaccca
    10321 gatgcgtaaa cgtaagcgtc tgatgaagca catcaagaaa aataaataat tatccgtaag
    10381 tattttatct gccattccct ctatgaatgt agagggaatg gctaccggag gaattcatat
    10441 ggaactgcat tgtccaaaat gtcagcatgt gcttgatcag gataacggcc atgcgcgttg
    10501 cccatcctgc ggggaattta ttgaaatgaa agctctttgt ccagactgcc atcaaccgtt
    10561 gcaggtgtta aaagcctgcg gtgcagtaga ttacttctgt cagcacgggc atggcttgat
    10621 atcgaaaaaa cgcgttgaat tcgtgctggc ttaatgaacc tttttacgcg atttttttac
    10681 cggctggatg ttttttactt cactttctat ccagccgtct tccagacgtg tggttagcat
    10741 ttcacccgtt ttaacttgct tcactttttt cagtacattg ccgtcagtag cagtagtaac
    10801 gctgtatcca cgcgccagcg ttgacagtgg gcttacggct tcgaggtgcg ttactgcatt
    10861 accgaaacgt tcacgcgtgg cgctaagctg tgcgcgcagg atttctgcta aacgatattc
    10921 cagttgctga atgcgcgttt gcgtgcgatg aatcttcggc tgtggatttt gctgattcag
    10981 ccgctgtgtt aatcgctgct gctgttgccc ggcacgctta agctgatttt ccagcgcaaa
    11041 gctcatccgt ttttgcaggc gttcaagcat ggtttgctgg cgtgccagcc ggagctgcgg
    11101 atgctgctgc tgcaagcgat gatgaatctg cgtaaagcga cgtgtacggt tggcgagata
    11161 ataatccatc gccatctcca gccgttgacg ggccgattgc acctggcgca gtaactcttg
    11221 ctgattacgg ctcactactt cagcggcggc agacggcgtt ggcgcacgca gatcggcaac
    11281 aaaatcggca atggtcacat ccgtctcatg cccgacggcg ctgacgaccg gaatgcggct
    11341 ggcaaaaatc gcccgcgcta cgcgttcgtc gttaaaactc cataaatctt ccagcgaacc
    11401 gccgccgcgc ccaacgatca acacgtcgca ctcattgcgc tgattcgcca gttcaatggc
    11461 gcgaacaatt tgccccggcg cgtcatcgcc ctgaacggag gtggggtaga tgatcaccgg
    11521 tagagaagga tcgcgacgtt ttaacacatg caaaatatca tgtagcgcag caccggtttt
    11581 tgaggtgatc acaccaacgc aatgcgcagg ggagggaagt ggttttttgt attgctgatc
    11641 gaacaaacat tcagcctgca acttcgcttt gagctgttcg tacttctgtt gcagcagccc
    11701 ttcaccggcc ggctgcatac tctcaacgat tatctggtag tcgccgcgcg gctcgtagag
    11761 cgtaatattg gcgcgaacta aaacttgttg cccatgctgc ggacggaagg tgacccggcg
    11821 gttgctgttg cggaacatcg cgcagcgtac ctgggcggtg tcgtctttga gtgtaaagta
    11881 ccagtgaccg gaagccggtt gtgtgaaatt agagatttcg ccgctgatcc aaacctgtcc
    11941 catctcatgc tcaagcagca gacgaaccgt ttgattcagg cgactaacgg taaaaattgc
    12001 aggggattga gaaggtaaca tgtgagcgag atcaaattct aaatcagcag gttattcagt
    12061 ctatagtaac ccgcccttcg gggatagcaa gcattttttg caaaaagggg tagatgcaat
    12121 cggttacgct ctgtataatg ccgcggcaat atttattaac cactctggtc gagatattgc
    12181 ccatgctacg tatcgctaaa gaagctctga cgtttgacga cgttctcctc gttcctgctc
    12241 attctaccgt tctgccgaat actgctgacc tcagcaccca gctgacgaaa actattcgtc
    12301 tgaatatccc tatgctttcc gcagcaatgg ataccgtaac ggaagcgcgc ctggctattg
    12361 ctctggctca ggaaggcggt atcggcttta tccacaaaaa catgtccatt gaacgccagg
    12421 cagaagaagt tcgccgtgtg aaaaaacacg aatctggtgt ggtgactgat ccgcagactg
    12481 ttctgccaac cacgacgctg cgcgaagtga aagaactgac cgagcgtaac ggttttgcgg
    12541 gctatccggt cgttaccgaa gaaaacgaac tggtgggtat tatcaccggt cgtgacgtgc
    12601 gttttgttac cgacctgaac cagccggtta gcgtttacat gacgccgaaa gagcgtctgg
    12661 tcaccgtgcg tgaaggtgaa gcccgtgaag tggtgctggc aaaaatgcac gaaaaacgcg
    12721 ttgaaaaagc gctggtggtt gatgacgaat tccacctgat cggcatgatc accgtgaaag
    12781 acttccagaa agcggaacgt aaaccgaacg cttgtaaaga cgagcaaggc cgtctgcgtg
    12841 ttggtgctgc agttggcgca ggtgcgggta acgaagagcg tgttgacgcg ctggttgccg
    12901 ctggcgttga cgttctgctg atcgactcct cccacggtca ctcagaaggc gttttgcagc
    12961 gtatccgtga aacccgtgct aaatatccgg atctgcaaat catcggcggc aacgtggcaa
    13021 cagctgcagg tgcacgcgct ttggcagaag ctggttgcag cgcggttaaa gtcggtatcg
    13081 gccctggttc tatctgtacg actcgtatcg ttactggcgt aggtgttccg cagatcactg
    13141 ccgttgctga cgcagttgaa gccctggaag gcaccggtat tccggttatc gcagatggtg
    13201 gtattcgctt ctccggcgac atcgccaaag ctatcgccgc tggcgcaagc gcggtgatgg
    13261 taggttccat gctggcgggt actgaagaat ctccgggtga aatcgaactc taccagggcc
    13321 gttcttataa atcttaccgt ggtatgggtt ccctgggcgc gatgtccaaa ggttcctctg
    13381 accgttattt ccagagcgat aacgctgccg acaaactggt gccggaaggt atcgaaggtc
    13441 gcgtagctta taaaggtcgc ctgaaagaga tcattcacca gcagatgggc ggcctgcgct
    13501 cctgtatggg tctgaccggc tgtggtacta tcgacgaact gcgtactaaa gcggagtttg
    13561 tacgtatcag cggtgcgggc attcaggaaa gccacgttca cgacgtgacc attactaaag
    13621 agtccccgaa ctaccgtctg ggctcctgat tctcttcgcc cgatttcatg tcgggcgatt
    13681 tatattatct gtttcacttg cctcggaata agcgtcaatg acggaaaaca ttcataagca
    13741 tcgcatcctc attctggact tcggttctca gtacactcaa ctggttgcgc gccgcgtgcg
    13801 tgagctgggt gtttactgcg aactgtgggc gtgggatgtg acagaagcac aaattcgtga
    13861 cttcaatcca agcggaatta ttctttccgg cggcccggaa agtactactg aagaaaacag
    13921 tccgcgtgcg ccgcagtatg tctttgaagc aggcgtaccg atattcggcg tttgctatgg
    13981 catgcagacc atggcaatgc agttgggcgg tcacgttgaa gcctctaacg aacgtgaatt
    14041 tggctacgcg caggtagaag tcgtaaacga cagcgcactg gttcgcggta tcgaagatgc
    14101 gctgaccgca gacggtaaac cgctgctcga tgtctggatg agccacggcg ataaagttac
    14161 cgctatcccg tccgacttcg tcaccgtagc cagcaccgaa agctgcccgt ttgccattat
    14221 ggctaacgaa gaaaaacgct tctatggcgt acagttccac ccggaagtga ctcacacccg
    14281 ccagggtatg cgcatgctgg agcgttttgt gcgtgatatc tgccagtgtg aagccctgtg
    14341 gacgccagcg aaaattatcg acgatgctgt agctcgcatc cgcgagcagg taggcgacga
    14401 taaagtcatc ctcggcctct ctggtggtgt ggattcctcc gtaaccgcaa tgctgctgca
    14461 ccgtgctatc ggtaaaaacc tgacttgcgt attcgtcgac aacggcctgc tgcgtctcaa
    14521 cgaagcagag caggttctgg atatgtttgg cgatcacttt ggtcttaaca ttgttcacgt
    14581 accggcagaa gatcgcttcc tgtcagcgct ggctggcgaa aacgatccgg aagcaaaacg
    14641 taaaatcatc gggcgcgttt tcgttgaagt attcgatgaa gaagcgctga aactggaaga
    14701 cgtgaagtgg ctggcgcagg gcaccatcta ccctgacgtt atcgaatctg cggcttctgc
    14761 aaccggtaaa gcacacgtca tcaaatctca ccacaacgtg ggcggcctgc cgaaagagat
    14821 gaagatgggc ctggttgaac cgctgaaaga gctgttcaaa gacgaagtgc gtaagattgg
    14881 tctggagctg ggcctgccgt acgacatgct gtaccgtcat ccgttcccgg gaccaggcct
    14941 tggcgttcgt gttctgggtg aagtgaagaa agagtactgt gacctgctgc gccgtgctga
    15001 cgccatcttc attgaagaac tgcgtaaagc ggacctgtac gacaaagtca gccaggcgtt
    15061 caccgtcttc ctgccagtac gttccgttgg cgtaatgggc gatggtcgta agtatgactg
    15121 ggttgtctct ctgcgtgctg tcgaaaccat cgactttatg accgcacact gggcgcatct
    15181 gccgtacgat ttcctcggtc gcgtttccaa ccgcattatc aatgaagtga acggtatttc
    15241 ccgcgtggtg tatgacatca gcggcaagcc gccagctacc attgagtggg aatgattttg
    15301 atccctgcac tatgaatgaa caaaaccctc cgttactaca gagggttttt tatcttcaag
    15361 aatcataggc ttgaagttac taacatcgat taattaaacc agctgtccga tttgttctct
    15421 tctgctttgc ccacgctttt catcagatcg cgaccgcctt cagtcatatt tctgtttgcg
    15481 tcagcttcag attgcaccac atcggtttgc gcagctttgt gcttcagttc ctgatcgata
    15541 aattcgtttt cgcgcttaac gcgggcttct tctttcgcca gcgccagttt ttgtttctga
    15601 atctctaagc tgcgtagctc atcttcataa ctttgatcgc gttttttgtc cgcagtggct
    15661 tcggcgtcca gtttatcctg acgagctttc ttatttgccg ctgccgttgc cgctcttttg
    15721 ttcgcggcgg cctgggcatt tgcgcgacgt tgcttctctt gctggatttc cctgttgcgc
    15781 tccgcgaccc attcgtcatg ctgcctttgc tcttcatttt taccttgctg ttccgcttct
    15841 gcgacagcag agagttgatc ctgcaatgat gaggctatag ccggatagct taaggaggct
    15901 aagatggcgc aaagaaaaac tttcttcatg actcctcctg attattagct cttttcagga
    15961 catttggtat ttggctgaat acgcgtttcg ttatacgttg tggtaataac aacggctaaa
    16021 cctgtcgtaa actggcactc tttaccctcc tgggtggagg tatacacttt ggtgccttcc
    16081 ttatatgtta aagaaacacc ttccactaag gttttatcat tcaccataga acccgctgcc
    16141 gcgcctacag ctccgccgcc aactgcccct gccgtcgttc cggaattgct gccagaaccg
    16201 acgttgtggc cgataacacc gccagcgact gcgccaataa gcgcgccgaa ggcttgtgcg
    16261 ttccgtttat tttgggagtt gtctacggca acttttgcgg gaagaatgga aataatatta
    16321 acggttttag tttcttgttt ggtattcagt tgatcggttt gataaacatc ggcggcatga
    16381 tcgtcagcat ttgactggca tcctgccaga gtgaatgacg ctaacattgc cacaggcaga
    16441 agacattttt taaatttcat cactattcct tgttatacac attacgaaaa ataagacatt
    16501 taagatcatc aaaagaggaa agtaaatgta ttgcgtataa agggatagat taaatttatt
    16561 aaaattcacg cagggaataa tttataaaaa tatatcatta caacatatat attaatttta
    16621 tatttatttc gcagccaaac aggataaaca tcagtcagga caaagacgta aaatttaaga
    16681 tgaaagaata tctgccgctt tgataatccc aaagagcaaa agcatccgac agcgaagtgc
    16741 cgttatgttc tgtgaaagtg gagaaggtaa ccgctaacgt tgtaccggtg attcgacgga
    16801 gagtgtggat tgctgagctt cttttttgct ctgatggtgc tgccaggcac cgacggacga
    16861 gtagataaaa cggccaaaga agaagataaa gctgatgagc agtacgatac gggtcatgcg
    16921 actgttaaat cggtgtcgtt ttcgcatact agttgcctga ctcacaaaag gttccttgaa
    16981 gtatgtccca cgctgtggac ggtacttaca ttaaggcaca acaggacaaa atggtcaatt
    17041 cttcgttatg taaaaaagcg tcagtggata catattttaa tgttatggaa gttaatttaa
    17101 ttatttacaa tgatgatgta ataatgatga attaacatga taaacgtaat aattcattaa
    17161 tcagggttat tttgtttgat atatatcaat tggaactttc atatgacact tagaatcaat
    17221 gctctctata gtgatgaata atcatcattc gaagtcaggt gggatgcctg tctgaataca
    17281 cctccttcag gatgtggggg attctgctga gcatctatga aactgaatgc aacttatata
    17341 aaaatacgtg ataaatggtg ggggcttccg ctgttcctgc cttctttaat cttgcccatt
    17401 ttcgcccaca ttaatacttt cgcgcatatt tcttccggtg aggtttttct cttttatctg
    17461 cctttggcac tgatgatcag catgatgatg tttttcagct gggcggcatt gccagggatc
    17521 accttaggga tttttgtccg caaatatgca gagctgggtt tttacgaaac gctctcatta
    17581 acggctaatt ttattatcat tatcattctc tgttggggcg gttacagggt ctttactccc
    17641 cggcgtaaca acgtttcaca tggtgatacc cgtttaattt cccagcgtat attctggcag
    17701 atagtgtttc ctgcaacgct gtttctgata cttttccagt ttgctgcatt tgtaggatta
    17761 ctggcgagca gagaaaatct ggtcggtgtc atgcctttta acctcgggac tttaatcaat
    17821 tatcaggcct tactggtggg taatctgatc ggtgtcccgc tgtgctactt catcattcgg
    17881 gtagtgcgaa atccatttta tttacgtagc tattattcgc aattaaaaca gcaggttgat
    17941 gccaaagtca ccaaaaaaga gttcgcgctc tggctactgg cattaggtgc tttattgttg
    18001 ctgttatgca tgccgttaaa tgaaaaaagc acaattttta gcaccaatta taccttgtca
    18061 ttattgctgc ccctgatgat gtggggagca atgcgctatg gttataagct gatttcgctg
    18121 ctctgggcgg tcgtgttgat gatcagcatc cacagctatc aaaattacat tcccatttat
    18181 cctggctata ccacgcagct gaccataacc tcctccagtt atctggtatt ctcttttatt
    18241 gtcaattata tggctgtact ggcaactcgt cagcgagcgg tagtcagacg cattcagcgg
    18301 cttgcgtatg tggacccggt ggttcatctg ccaaatgttc gcgccctgaa tcgcgcgttg
    18361 cgtgatgccc cctggtctgc gctttgttat ttacgcatcc ctggcatgga aatgctggtt
    18421 aagaactatg gcatcatgct gcggattcaa
//
LOCUS       DAEBXN010000083        18219 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.083, whole genome shotgun sequence.
ACCESSION   DAEBXN010000083 DAEBXN010000000
VERSION     DAEBXN010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18219
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.083"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(62..820)
                     /gene="aroD"
                     /locus_tag="KJL99_002904"
     CDS             complement(62..820)
                     /gene="aroD"
                     /locus_tag="KJL99_002904"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBD4437564.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLVGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(851..1717)
                     /gene="ydiB"
                     /locus_tag="KJL99_002905"
     CDS             complement(851..1717)
                     /gene="ydiB"
                     /locus_tag="KJL99_002905"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HBD4437565.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTDTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1729..3000)
                     /locus_tag="KJL99_002906"
     CDS             complement(1729..3000)
                     /locus_tag="KJL99_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD4437566.1"
                     /translation="MRTVTKLSFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLSEK
                     FSTDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPN
                     LTLAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSY
                     MLLNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGV
                     SSVLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLK
                     KMVRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEF
                     FPKSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFS
                     RYYRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(3183..4388)
                     /locus_tag="KJL99_002907"
     CDS             complement(3183..4388)
                     /locus_tag="KJL99_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD4437567.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFDILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSTGSIATFTIPLITAHLSQRSIADIMWFDTAIGFLLALFIGLRSRKKTRHHS
                     LKENVAPGG"
     gene            complement(4488..4844)
                     /locus_tag="KJL99_002908"
     CDS             complement(4488..4844)
                     /locus_tag="KJL99_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HBD4437568.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(4980..5087)
                     /gene="rprA"
                     /locus_tag="KJL99_002909"
     ncRNA           complement(4980..5087)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="KJL99_002909"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5273..6385)
                     /gene="ydiK"
                     /locus_tag="KJL99_002910"
     CDS             complement(5273..6385)
                     /gene="ydiK"
                     /locus_tag="KJL99_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HBD4437569.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            6774..9830
                     /locus_tag="KJL99_002911"
     CDS             6774..9830
                     /locus_tag="KJL99_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBD4437570.1"
                     /translation="MIPQISLAPGVVQLVLNFLQELEQQGFTGDAATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDMPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9827..10237
                     /gene="menI"
                     /locus_tag="KJL99_002912"
     CDS             9827..10237
                     /gene="menI"
                     /locus_tag="KJL99_002912"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HBD4437571.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10337..10525
                     /locus_tag="KJL99_002913"
     CDS             10337..10525
                     /locus_tag="KJL99_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HBD4437572.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            11073..11441
                     /gene="sufA"
                     /locus_tag="KJL99_002914"
     CDS             11073..11441
                     /gene="sufA"
                     /locus_tag="KJL99_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HBD4437573.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQSMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFRV"
     gene            11450..12937
                     /gene="sufB"
                     /locus_tag="KJL99_002915"
     CDS             11450..12937
                     /gene="sufB"
                     /locus_tag="KJL99_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HBD4437574.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12947..13693
                     /gene="sufC"
                     /locus_tag="KJL99_002916"
     CDS             12947..13693
                     /gene="sufC"
                     /locus_tag="KJL99_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HBD4437575.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13668..14939
                     /gene="sufD"
                     /locus_tag="KJL99_002917"
     CDS             13668..14939
                     /gene="sufD"
                     /locus_tag="KJL99_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HBD4437576.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            14936..16156
                     /gene="sufS"
                     /locus_tag="KJL99_002918"
     CDS             14936..16156
                     /gene="sufS"
                     /locus_tag="KJL99_002918"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HBD4437577.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGQQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVE
                     RLVTGLQRIHRLLG"
     gene            16169..16585
                     /gene="sufE"
                     /locus_tag="KJL99_002919"
     CDS             16169..16585
                     /gene="sufE"
                     /locus_tag="KJL99_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HBD4437578.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDGSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16734..17738
                     /gene="ldtE"
                     /locus_tag="KJL99_002920"
     CDS             16734..17738
                     /gene="ldtE"
                     /locus_tag="KJL99_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HBD4437579.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17801..18037)
                     /gene="lpp"
                     /locus_tag="KJL99_002921"
     CDS             complement(17801..18037)
                     /gene="lpp"
                     /locus_tag="KJL99_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HBD4437580.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
BASE COUNT         4791 a         4402 c         4681 g         4345 t
ORIGIN      
        1 acggttaaaa atactttttt tatttcggcg ggggagggta tttccgccga aatattattg
       61 cttatgcctg atgtaaaata gttaataccg tgcgcaaatc atttaccgag atttgccctg
      121 gcgcagaggc tttttttacc gcaccaaaag ttgccgccga gccaaatact tcaccaacca
      181 gacgagaaat tacgccagtt tttgccatcg acatcgtgat gattggacga tcggcatact
      241 gctcctgcat ctccagggtc gcggcaagca acgtcagcac atcgctggta ctttgcggca
      301 tcagcgcaat cttaggaata tcggcgtcga aggactgcat tttgcgcaga cgggcaatga
      361 tttcttcggc ttccggcgtt ttatggaagt catggttgga catgacaact ttcacatcat
      421 gcgcgtgggc gtaggcgacg gtttctttga cctgatcatc gccggtaaat aactccagat
      481 cgatcatatc aaccaggccg ctgtcgatgg ctgcacgatt gagagcaata taagcctcgg
      541 tggaaatcgc ctgctcgccg ccttctttgg cactgcggaa ggtaaacagc agcggttttt
      601 ctggcatggt ttcacggaga atttttgccg ccgccatgac agactccaca ttggagaggt
      661 cggcaaagtg gtccacacgc cattccagaa tatcaaagtc cgcttcacga taggcgagag
      721 cttcggattt cacgcgggcg atatctttcg ccatcagcga gacgatgatt ttaggtgcgc
      781 ccgcaccaat gacgagatct tttacagtta cggttttcat tttttaccct ttctgcacgc
      841 ggtcagcctg tcaggcaccg aaccccatga cctgtttaac atattccaga gggaaatctt
      901 tgccagtcca taacgtgaac tgttcagccc cttgccacaa caacatgccg tatccatcaa
      961 tcgttttgca accagcttgt tgtgcctgct gcaataactt cgtcatatgc gggttataca
     1021 cgcattcagt gaccagcagt cccggatgta acagactgat atcattaacc aatgattcat
     1081 tctcaagggg tttcatacct acttttgtgc cattggttaa aatgtcggcg gaagccaggg
     1141 cttcagcaaa ggcttgctga tcggcgagat cggtatccgt gacgacacaa tcggtgtttt
     1201 cgttaacccg ctgcgcgaag gcgagggctt tatcgaagaa ctcatcccga cggttaaaga
     1261 gtttaatttc ttttaaacct tcaattgccc cctgcgcgcc aatcgccgtt gaggcacctc
     1321 cggcccctaa cagcaccatc gttttgcctt tgatatcaaa accgctctct ttaatggcgc
     1381 gaatatggcc cgtgccgtcg gtgttatagc cacgcagata gccatcatca ttaacgatgg
     1441 tgttgatggc acccaccagt ttggctgctg gcgttaattc atcaacatat tcacacgcca
     1501 gttgtttgtt cggcatcgac acaccagttc cgcgcatttt gagggctttt aatccttcaa
     1561 ttgctccggg aaagctatcg ttatccactt cgaaggccat ataggtaaat ggcaatcccg
     1621 ctttttctaa ggctttattc tgcatttcag gcgataaact gtggcggata ggataagcca
     1681 tcaatccaat caattcgtat tttgcggtaa catccataat taactccttt aacctctatg
     1741 cttaattgtg tttaaccggg tgctaaattt gcgttcgcca aaccgcacat cattctcagg
     1801 aataataaaa acgcggtaat agcggctaaa aacaataatt gcggtaatca gggccagcag
     1861 cgcgaaagta aaatcgagaa caatgatata ttgcaggccg atgttcgaca gataaccggt
     1921 aatcagcgga ataacaaagt tagccagtcc acccatcatc atataaatac tggtgacttt
     1981 ggctttgctt ttggggaaaa actctgacat gaccgaaaca ccgagctgta aaatgccgcc
     2041 agctgctgag aaaccgataa caaaggcgcc ggcattacac accagtggag aagggtacag
     2101 gtagataatg gcagctgtta ttgttgccag cgcagagtta aatacattag cccagatggg
     2161 ccggaccatt tttttcagta gtgcggcaaa aataaagaca cagaccaacg agcccatact
     2221 gtaataagag atggttttta atgcctcagc ttctgacata ccagcaaaag ccatcgcata
     2281 tttgggcatc cacaccacaa tcacataaaa ggtcgagaat gcggctacac cgaacagtac
     2341 cgatgaaaca ccttccagcc agactaaagg tttgctgttc atttgcggta attcattagc
     2401 tacgctggcg tccaccaact ggctggggaa tttgcttttc aacagcatca gcgtgatcag
     2461 tacaaacaga atacccggaa taatcagccc atagccgtac cagatattat tgagcaacat
     2521 atagctcacc agcattgggt agaacatttg cccaaatgac accatcgctt taaccagtat
     2581 gaccgccgaa ccagaggctt tcggaaagca ttccatgagc gcggggtagc cacccgtatc
     2641 cagcgctgag ttagcgatac ctacgcacac tgccagaccg taggcgagag ttaaattcgg
     2701 gcaagcggga ataccaaaga agaatagcag atacattatt actgccatta atatcaccgc
     2761 ccgacgacca aacttatcgg agatcacacc gaagaataaa atactgatca atcgccccaa
     2821 accgataccg gaaattaaat aggcaatgcc cgcgttgtca gtggaaaact tttctgacag
     2881 agatgtcata ttttgggcaa gcgtaataac actaatgccg tgcaggaaat agctgaagta
     2941 aatacaaaga acagccagga taaatggcgt gctgaaagat aatttcgtta ccgtacgtat
     3001 tgttctccga cggtttacat aattactata ctgaagatga ttttccctgg gtacatggca
     3061 taaagtttat gtttccagga atattgcatg tcatagtttg tttttgatcg cgattttcct
     3121 tagttagcgc agaaagagtt atttatccgt aagatcttgc atgacctgaa aagaatattg
     3181 cattacccac ccggagcgac attttccttt agcgagtgat gccgcgtttt tttgcggctg
     3241 cgtaagccga taaacagtgc cagtaaaaaa ccgatggcag tatcgaacca cataatatcg
     3301 gcaatacttc tttgggacag atgagccgta atcaacggaa tagtaaaggt cgcaatactg
     3361 cccgtactgt aatagatccc tgtagcttta cctttagcgt aagggaaacg ttcagccatt
     3421 aacgtcaggc caatttgcac aacacctcca gcagaggtaa aaccaatgac aaaagcaaat
     3481 attatcacca cataaaatgt gggatgcagg cagacggtaa acagagcaat aaatgagata
     3541 aaggtgtaca gcatcagtaa tgttgttggg cgaacggtat tacgaatgag tggagcggta
     3601 ataaatacac aaagcagcga acccacggtg tagatactga gtagtttgat cgacatagtg
     3661 tatgacatgc ctgcaacaaa ttgtccgtac tgtgccagcc actggctaac cagataaaac
     3721 gttgccattg agatatagcc atataaggta taactggcta aatcgataat tgaacagcga
     3781 tgttccgtag agctggtggt tttctttatg acaggtaagc gacgacccgg atggggtggg
     3841 aacgtacaac ggtataaaaa cagagcgtta ataaacataa tgcctgcagc aatcataaag
     3901 gaccaaccga accacagttc agcccacact aacaggctaa tgattagcgg taataaaaat
     3961 tgtccgctgg aaacaaatgc tttaattaaa atattggctg tcccaggtga gcgtggaaaa
     4021 gcttccatca aactgggata agtgcctgca tcgagaaaac tgtttgccat tcccgccaga
     4081 aagccaaaaa cataagcgat aatgatgtta ttggtctgca ggatgtcaaa aaagaaggcc
     4141 atatagcagc acatcccgag catgataaaa gggcggcgac caaagcgatc ggataataat
     4201 cctgcaaaaa gcaagacact taatcgacca atgcccagcg atgagataac tatcgagaca
     4261 cccgcggcat tagtctgcca aagtgtctcc agcgaggcca tattcaggct catcaaaatg
     4321 acgcccatac catgcaccag gtaattaaaa tacaacccaa gtgcggtagg gtaataggga
     4381 tttttcatag gttctactta gcaaaggtgc tttaaaaagt gtagcgatgt tgtgtcagta
     4441 tccattcatt attgttgtaa caacgacaat tcctttctgt ggaaaaatta acaaagccgc
     4501 tccagatcgt gtgcatataa ttcagcggct accgattgat agatcttcca gtcaataaaa
     4561 ttgccatcag ggtagacttg ttgaaacgcg gttaagtcgg ggaaaaaacg catggtgttg
     4621 ttgccgatgc ggttatttat tttctctatg atggcatgaa gatgtttttt tcgttgctga
     4681 cgcatagcca acagttgctc gactacacaa tcaggaatgg cgcatttgcc attttcccac
     4741 tgacgccagc tttcactgtc accggtctgg gcaatccagg tcgcgcattc atcaatagtc
     4801 atggcaaaaa tatggcggag tgcctggagt tcataagcgt tcatcgtgta atggctgcat
     4861 gaaataaaca ataaaatata aataacatta tcagcctgct gacggcttga agagagtcac
     4921 agtatcttgt gcaacattat tggaaagagt gaggggcgag gtagcgaagc ggaaaaatgt
     4981 taaaaaaaag cccatcgtgg gagatgggaa aagactacac acagcaattc gttgtttcac
     5041 tcaggggatt tccatgctta taaatcaata tgttgattta taaccgtgag ctaatagtag
     5101 gcatacggac tttttgcgtc gatcagattc gtctcattag tttaaaaact gtaaagaatt
     5161 tgcgacaggt actaccagct gaatggcggt aagcaggaaa atatggggaa gagcaagcaa
     5221 atatcaatta gcggtgatta acaatcaggc ggcagagccg cctgatgaga aattacttat
     5281 tcggtttctc gatttcgcca agttcttcga gaatttcttc cggttggtcc gtcggtggcg
     5341 ggacttcttc cacccacgcg gcaaacagac gccaggaaac ggctaacaga accggaccaa
     5401 taaacagacc gatcatcccg aaagcaatca aaccaccaat aacgccagag aggatcagga
     5461 tcagcggtaa atcggcaccc atgcgaatta acattgggcg gatgacgtta tccagcgtgc
     5521 caaccacacc gctccacact aacaataccg ttccccaggt ggtatcgccg gtccagtaga
     5581 gccagataat cgccggaatc agtaccggca acgggccaag ctggacaagg caggagagga
     5641 tcattaacac cgttagcaac gttgcataag gtacgccgga tacggcgagg ccgataccgc
     5701 caagcactgc ctgtactaac gccgtcacca ccacacccag cgccaccgcg cggatagcct
     5761 gtgccgccag cagcacggcg gcatctccgc gaacgcctgc cagacgggtt gcaaaatggc
     5821 gaatgccttg tgccacctgt tcaccgcgcc agtacagcag ggcactgaag agaagcatca
     5881 gcgcacaatg caccataaag cgcccgatat gcgccgcctg cccaacgaac caggtggtgg
     5941 tggtgccaat ataagggcgg actttcgcca tgatcgccgt accccccata tccagcaagt
     6001 tgtgccagcc tgcatacagc ttcgcgccaa tcaccggaat ggtattaagc cacgccagat
     6061 cgggtaacgt catgtcgccg ctggaaatgg ctttaattag cgggccgctg ccgtcgacga
     6121 tactgttaac cagcaaagca atagggatga taaacaccat cactaataac agcgtcatca
     6181 ccagaacggc gagagagcgg cggccaaaca tgatcttttg caaacgtaac aataccggcc
     6241 aggtggcgat aaccaccgta ccggcccatg caaagccgag aataaagggt tgaacaatcc
     6301 acagacatgc cacaatcatg atggctaaaa acagcaccga aagcagaatt tgtgcgacat
     6361 ccctgggctg acgaacattt accataacta cttttcacct tttttgtgcg tcaaatcgtc
     6421 gacgcgacgt gaacacgcga aactcacctg cataatgatg agcaatttca gcggttttta
     6481 acaggccgat tctgccctta attctgatgg gcgtgttaga aaaaaatgtg gtacaacaac
     6541 tcaggcaaca cgcaaacgat ttactcgtcg tatttcaagt tgcatgtgct gcggctacgt
     6601 tcgttcaccc cagtcactta ctattgtaag ctcctggagt tcactcgctt gccgccttcc
     6661 tgcaactcga aatccataga gtaaataaac atcacctttc agtgcgctat tgcagccgcc
     6721 tgaaagaata gtgtatcgac ctacatcaca gaccgcagga aagggtcaat ataatgattc
     6781 cacagatttc cctggcaccc ggcgtcgttc aactggtgct taattttttg caagagctgg
     6841 agcaacaagg ttttaccggc gatgcggcga caagttatgc cgatcgtctg acaatgtcga
     6901 ccgacaacag tatttaccaa cttctccccg atgcggtggt atttccgcgt tcaaccgcag
     6961 atgtggcgct gatcgcccgt cttgccgcgc aggaacgcta ttcatcgctg atctttaccc
     7021 cccgcggcgg cggcaccggc actaacggtc aggcgctcaa ccaggggatt attgttgata
     7081 tgtcccgcca tatgaaccgc atcatcgaaa ttaaccctga agagggctgg gtgcgcgttg
     7141 aggccggggt gataaaagac caactcaatc agtacctgaa accgttcggc tacttttttg
     7201 cgccggaact ttcgaccagc aaccgggcaa cgctcggtgg gatgatcaat accgatgcat
     7261 ccggtcaggg atcgctggtc tatggcaaaa cgtcagatca cgtacttggc gtacgcgcgg
     7321 tgttgttggg gggcgatatt ctcgatacgc aacctttacc cgtcgagctg gcggaaacgc
     7381 tgggtaaatc caataccaca atcgggcgaa tttataacac ggtttatcaa cgttgccgtc
     7441 agcaacgcca gttaatcatc gacaacttcc ccaaacttaa ccgctttctt accggttacg
     7501 atctgcgtca tgtctttaac gatgagatga ccgagttcga cctgacgcgc attctgacgg
     7561 gttcagaagg gacgctggcc tttattaccg aagcgcggct ggatattacg cgcttgccta
     7621 aagtgcgccg tctggtgaat gtcaaatatg actcttttga ctctgcgctg cgtaacgcgc
     7681 cgtttatggt tgaggcgcgg gcgctttcgg tagagacggt ggactcaaaa gtgctgaatc
     7741 tggcgcggga agatattgtc tggcattccg tcagcgagtt gattaccgat atgcctgaca
     7801 aagagatgct cggactgaac attgtggaat ttgctggtga tgatgaggcg ctgattgacg
     7861 agcgagttaa tgcactctgt gtgcggcttg atgagctgat tgccagccaa caggcaggtg
     7921 tgattggctg gcaggtgtgc cgcgagctgg cgggggttga acgtatctat gcgatgcgca
     7981 aaaaagccgt tggtctgctt ggcaatgcca aaggtgccgc taagccaatt ccgtttgctg
     8041 aggatacctg cgtaccgccg gaacatctgg ccgattatat tgctgaattt cgcgcgctgc
     8101 tcgacagcca cggcttaagc tacggtatgt tcggtcacgt cgacgcaggt gtcttgcacg
     8161 tccgtccggc actggatatg tgcgatccgc aacaagagat tttgatgaag caaatctctg
     8221 atgacgtggt ggcgctgact gcgaaatacg gtggtttgtt gtggggcgag cacggcaaag
     8281 gttttcgcgc tgaatacagc ccggcgtttt tcggtgagga actttttgca gaactgcgca
     8341 aagtgaaagc ggcatttgac ccgcataacc gactcaaccc agggaagatt tgcccgccag
     8401 aaggtctcga tgcgccgatg atgaaagtgg acgcggtgaa gcgcggtaca ttcgatcggc
     8461 agatccccat tgcggtacgc cagcagtggc gcggtgcgat ggagtgtaac ggcaacggtt
     8521 tatgcttcaa ctttgatgcc cgtagtccga tgtgtccgtc gatgaagatc acccagaacc
     8581 ggattcattc accgaaaggg cgcgcaacgc tggtgcgtga atggctgcgc ttgttggcgg
     8641 atcgcggcgt tgacccactc aaactggaac aagaactgcc tgaatccggc gtcagtttgc
     8701 ggacgttaat tgcccgcacg cgcaatagct ggcatgcgaa taaaggcgaa tatgacttct
     8761 cacacgaagt caaagaggcg atgtcgggct gtctggcctg taaagcttgt tcgacccagt
     8821 gccccatcaa aattgatgtg ccggagtttc gctctcgttt tctgcagctc tatcacaccc
     8881 gttatttacg cccgcttcgc gaccacctcg ttgctacggt cgagagctac gcaccgctga
     8941 tggcacgcgc gccgaaaacc tttaacttct tcattaacca gccgctggtg cgcaaactct
     9001 cggaaaaaca tatcggcatg gttgatttgc cactgctgtc ggtcccctcg ctacaacaac
     9061 aaatggtggg gcatcgctcg gcaaacatga cgctggaaca gcttgaagct ctcaatgcag
     9121 agcagaaagc gcgcacggtg ttggtggtgc aggacccctt taccagctat tacgatgcgc
     9181 aagtggtggc ggattttgtc cgtctggttg aaaaattagg tttccagcct gtgttactgc
     9241 cattttcgcc aaatggcaaa gcccagcata tcaaaggctt tcttaatcgt tttgcgaaga
     9301 cggcgaaaaa gacggcggat ttcctcaacc gtatggctaa gcttgggatg ccaatggtgg
     9361 gcgtcgatcc ggcgctggta ctttgttatc gcgatgaata taaactggcg ctgggcgagg
     9421 aacgtggcga gtttaacgtc ttacttgcga atgaatggct ggcaagcgca cttgagtcac
     9481 atccagtggc tacagtcagc ggtgaatcat ggtatttctt tggtcactgt accgaggtta
     9541 ccgctttgcc gggcgcgcca gcacaatggg ccgcgatatt tgcccgtttt ggcgcgaaac
     9601 tggaaaatgt cagcgtgggt tgctgcggca tggcagggac ttacggacat gaagcgaaaa
     9661 accatgaaaa ttcgctcggg atctatgagt tatcctggca tcaggctatg cagcgactgc
     9721 cgcgtaaccg ctgtctggcg accggatatt cctgccgtag ccaggtaaaa cgggttgaag
     9781 gcacgggggt acgccatcct gtgcaggctt tactggagat tattaaatga tatggaaacg
     9841 gaaaatcacc ctggaagcac tgaatgctat gggtgaagga aacatggtgg ggttcctgga
     9901 tattcgcttt gaacatattg gtgatgacac ccttgaagcg acaatgccag tagactcgcg
     9961 gacaaagcag cctttcgggt tgctgcatgg aggagcatcc gtggtactgg ccgaaagtat
    10021 cggttccgtt gccggttatt tatgtaccga aggtgagcaa aaagtggttg gtctggaaat
    10081 caatgctaat cacgtccgct cggcacgaga agggcgggtg cgcggcatat gtaaaccgtt
    10141 gcatctcggt tcgcgtcacc aggtctggca gattgaaatc ttcgatgaga aagggcgttt
    10201 gtgctgttcg tcacgattga cgaccgccat tttgtgacgt tgtcaccaga aaagtgtgac
    10261 gcatattgct tttggtcatt atctgcacag taaacggtgg tatactgtgc aggtcttgtc
    10321 taaaagcgag gatgctatgt ctactcaact tgatcccacc cagctggcca ttgaattttt
    10381 acgccgtgac cagagcaatc tttcacctgc ccaatatctc aaacgtctga aacaactaga
    10441 acttgaattt gctgacctgc ttactctctc ttctgctgaa ttaaaagaag aaatctactt
    10501 tgcctggcgt ctgggcgtgc attgatcctg acttaatgcc gcaagttcgc caaatcgacc
    10561 tggattaacc tccgtccagg ttcgatgacc ttcttaatac tgcttatcta ccgcctgata
    10621 ttgttcaaaa ataatactaa tcgcagtttg tgttaaaacg gcgggttagc tttatgagat
    10681 tattcttatc gccccttcaa gagctaagcc actgagagtg ccggagataa gcgccggatg
    10741 gggtagaaac ccttaagcct gtgtcgcaca gacttaaggg ttttcttatt tctgaacgct
    10801 gcactttttt acctatctct taaatagtca ttttcaatac aactttttct ttttcatcac
    10861 agccttaatg ctccttagat aataaccatt atctaacaat gagataccta attcttagcg
    10921 tgcctgttaa cccacacatc aggggctatg cttattaaaa gcaggcaaaa agaatgtttt
    10981 acggtaaagc ccctgcgttt gctgggttga actgataatc attatcacta acatgctgtt
    11041 atacgctgaa agcgatgaag tgaggtaaat agatggacat gcattcagga acctttaacc
    11101 cacaggattt cgcctggcaa ggcttaacgc tgacacccgc agcggcgata cacatccgcg
    11161 agctggtggc aaagcagcca ggtatggtcg gcgtgcgctt aggtgtgaag caaacgggct
    11221 gcgcgggctt tggctatgtg ctcgacagtg ttagcgagcc ggacaaagac gatctgctgt
    11281 ttgaacacga cggcgcgaag ctgtttgtcc cgctgcaatc gatgccgttt attgatggca
    11341 cggaagtcga tttcgttcgt gaaggactta atcagatatt caaatttcac aaccctaaag
    11401 cccagaatga atgtggctgt ggcgaaagct ttagggtata ggcggtacta tgtctcgtaa
    11461 tactgaagca actgacgatg tcaaaacctg gaccggcggc ccgctgaact ataaagaagg
    11521 attcttcacc cagttagcca ccgatgagct ggcaaagggg ataaacgaag aggtggtgcg
    11581 cgcaatttcg gcgaagcgta atgagccgga gtggatgctg gagtttcgtc taaacgccta
    11641 tcgcgcatgg ctggagatgg aagaaccgca ctggttgaaa gcgcactacg acaagctgaa
    11701 ttatcaggat tacagctact actcagcacc atcgtgcggt aattgtgacg acacttgcgc
    11761 gtctgaacct ggcgcggtgc agcaaactgg cgcgaacgcc tttttaagta aagaggtgga
    11821 ggcggcgttt gagcagttgg gcgttcccgt gcgggaaggc aaagaggtgg cggtggatgc
    11881 cattttcgac tctgtttcgg ttgccaccac ttatcgcgaa aaactggcgg agcagggaat
    11941 tattttctgt tcctttggtg aggcgatcca cgatcacccg gaactggtgc gtaaatatct
    12001 cggcaccgtg gtgccgggga atgacaactt ctttgcagca cttaatgcgg cggtagcctc
    12061 tgacggtacg tttatttatg tgcctaaagg cgtgcgctgc ccgatggaac tttccaccta
    12121 ttttcgcatt aacgcggaaa aaaccgggca gtttgagcgc acaattctgg tggccgacga
    12181 agacagctac gtcagctaca ttgaaggctg ttccgctccg gtgcgtgaca gctatcagtt
    12241 gcacgcggca gtggtggaag tcatcatcca taaaaacgcc gaggtgaaat attccacggt
    12301 acaaaactgg ttccctggcg ataacaacac cggcggtatt ctcaacttcg tcaccaagcg
    12361 tgctttgtgc gaaggcgaaa acagcaaaat gtcatggacg caatcagaaa ccgggtcagc
    12421 gattacgtgg aaatatccca gctgcatttt gcgcggcgat aactccattg gtgagtttta
    12481 ctcagtggcg ctgaccagcg gtcatcagca agcggatacc ggcaccaaga tgatccacat
    12541 cggtaaaaac accaaatcga ccattatctc gaaagggatc tctgccggac atagtcagaa
    12601 cagttatcgc ggcttagtga aaatcatgcc gacggcaacc aatgcgcgca atttcactca
    12661 gtgcgactca atgctgattg gcgctaattg tggggcgcat accttcccgt atgtcgaatg
    12721 tcgcaataac agcgcacaac tggagcacga ggcaacgaca tcacgtattg gtgaagatca
    12781 actgttttac tgcctgcaac gcgggatcag cgaagaagac gccatctcga tgattgttaa
    12841 cggtttctgc aaagacgtgt tctcagaact gccgctggaa tttgccgttg aagcacaaaa
    12901 actcctcgcc atcagtcttg aacacagcgt cggataagga ataaacatgt taagtattaa
    12961 agatttacac gtcagcgtgg aagataaagc tatcctgcgt ggattaagcc tcgacgttcg
    13021 tcccggcgaa gttcacgcca ttatggggcc aaacggttcg ggcaaaagta ccttatcggc
    13081 aacgcttgcc gggcgcgaag attatgaagt gacgggcggc acggttgagt tcaaaggcaa
    13141 agatttgctt gcactgtcgc cggaagatcg tgcaggcgaa ggcatcttta tggccttcca
    13201 gtatccggtg gagattccag gcgtcagtaa ccagtttttc ctgcaaacgg cacttaatgc
    13261 agtgcgcagc tatcgcggcc aggaaacgct cgaccgcttt gattttcagg atttgatgga
    13321 agagaaaatc gctctcctga agatgccgga agatttatta acccgttcgg taaacgttgg
    13381 tttttccggc ggcgagaaaa agcgcaacga tattttgcaa atggcggtgc tggaaccgga
    13441 gttatgcatt cttgatgagt cggactccgg gctggatatt gacgcattaa aagtggtcgc
    13501 cgatggcgtg aactcgctgc gtgatggcaa gcgctcattc atcattgtta cgcactacca
    13561 acgcattctc gactacatca agcctgatta cgttcatgtg ctatatcagg gacgaattgt
    13621 gaaatccggc gatttcacgt tggtcaaaca actggaggag cagggttatg gctggcttac
    13681 cgaacagcag taatgcgctg caacagtggc atcacttgtt tgaagctgaa gggacaaaac
    13741 gctcaccgca agcacagcag catttacaac aattgctgcg taccggactg ccgacacgta
    13801 aacatgaaaa ctggaaatat acgccgctgg aagggctgac caatagtcag tttgtcagca
    13861 ttgcgggaga tatatcccca cagcagcgtg atgccttagc gttaacgtta gacgccgtgc
    13921 ggctggtgtt tgtcgatgga cgttacgtgc ccgcgctgag cgatgcaatt gaaggcagcg
    13981 gatatgaagt gagcattaac gacgaccgtc aggggttacc cgacgctatt caggcggaag
    14041 tgtttctgca tttgacggaa agcctggcac aaagcgtgac gcatatcgcc gtgaagcgcg
    14101 gtcaacggcc ggcaaagcca ttgctgttaa tgcatatcac ccagggcgtg gcaggtgaag
    14161 aggtgaacac tgcccattac cggcatcatc tggatctggc ggaaggtgcc gaagcaacgg
    14221 tgatcgaaca ttttgtcagc cttaatgatg ctcgtcattt taccggggca cggttcacta
    14281 tcaatgtcgc agcgaacgcc cacttgcagc atatcaagct ggcgtttgaa aacccggtca
    14341 gtcaccactt tgcccataac gatttgttgc tggctgacga tgccaccgca tttagccata
    14401 gtttcctgct gggtggcgca gtgttacgac acaacaccag tacgcaactc aatggcgaaa
    14461 acagcacgct gcggatcaat agcctggcga tgccggtgaa aaacgaggtg tgtgataccc
    14521 gtacctggct ggaacacaat aaaggttttt gtaacagccg acagttgcac aaaactatcg
    14581 tcagcgacaa aggccgcgcg gtatttaacg gtttgatcaa cgtcgcgcag cacgccatca
    14641 aaacggatgg tcagatgacc aacaataatc tgctgatggg caaactggcg gaagtggata
    14701 caaaaccgca gctggaaatc tatgcagatg atgtgaaatg cagccacggc gcgacggtgg
    14761 ggcgtattga tgatgaacag atgttctatc tgcgctcgcg cgggatcaat cagcaggatg
    14821 cccagcagat gatcatttac gcctttgctg ccgaactgac ggaagcactg cgtgatgagg
    14881 ggcttaaaca gcaggtgctg gcccgaatcg gtcaacggct gccaggaggt gcaagatgac
    14941 tttttccgtc gacaaagtgc gggccgactt tccggtgctt tcgcgtgagg taaacggttt
    15001 gccgctggct tatctcgaca gcgccgccag tgcacagaaa ccgagccagg tgattgacgc
    15061 cgaggccgag ttttatcgtc atggctacgc ggcggtgcat cgcggtattc ataccttaag
    15121 cgcccaggcg accgagaaaa tggagaacgt gcgcaagcgg gcatcgctgt ttattaatgc
    15181 ccgttcggcg gaagagctgg tgtttgtccg cggcacgacg gaagggatca atctggtcgc
    15241 caatagctgg ggcaacagca atgtgcgggc gggcgataac atcatcatca gtcagatgga
    15301 gcaccacgct aacattgtcc cctggcagat gctttgcgca cgcgttggcg cagagctgcg
    15361 tgtgatccct ctcaaccccg acggtacgct gcaactggag acgctgccta cgctgtttga
    15421 tgagaaaact cgcctgctgg caattactca tgtctccaac gtgcttggca cagaaaatcc
    15481 actggcggaa atgatcacgc ttgcgcacca gcatggcgca aaagtgctgg tggatggcgc
    15541 tcaggcggtg atgcatcatc cggtggatgt tcaggcgctg gattgcgact tttacgtgtt
    15601 ctccgggcat aaactgtatg gccccaccgg aattggcatt ctttatgtga aagaagcctt
    15661 gttgcaggag atgccgccgt gggaaggggg cggttctatg atcgccaccg tcagcctgag
    15721 tgaaggcact acctggacca aagcaccatg gcggtttgaa gccggtacac ccaataccgg
    15781 gggcatcatt ggtcttggcg cggcgctgga gtatgtttcg gcgctggggc ttaatagcat
    15841 agccgagtat gaacagaatc tgatgcatta tgcgctatca cagctggaat ctgtaccgga
    15901 tctcactctc tatggccaac aaaacaggct tggcgttatt gcttttaatc tcggtaaaca
    15961 ccacgcctat gatgttggca gttttctcga taattacggc attgctgtgc gtaccggaca
    16021 tcactgcgct atgccattaa tggcctatta caacgtccct gcgatgtgtc gggcgtcgct
    16081 ggccatgtat aacacccatg aagaagtgga gcgtctggtg accggcctgc aacgtattca
    16141 ccgtctgctg ggataacagg gaggcactat ggctttattg ccggataaag aaaaattgct
    16201 gcgtaatttt ttacgctgcg ccaactggga agagaaatat ctctacatta ttgagctggg
    16261 ccagcgtctg ccagaattac gcgccgaaga cggaagccca caaaatagca ttcagggctg
    16321 ccagagtcag gtgtggattg tcatgcgcca gaatgcgcag ggaattattg aattgcaggg
    16381 cgacagcgat gcggcgattg tgaaagggct tattgcggtc gtctttattc tctacgatca
    16441 gatgacgccg caggatattg tcaatttcga tgtgcgtccg tggtttgaaa aaatggcgct
    16501 cacccaacat ctcaccccat ctcgttcaca aggtctggaa gcgatgattc gcgcaattcg
    16561 cgccaaagcc gctgcactta gctaaactaa agggacagct ttcatccggt tggctcacat
    16621 tgccggatgg tcaatgttta cactgaccgg cacaggagtg ttttggtttc aggtgaacat
    16681 aagcaccatc cgacgtttca gcgtgagtct ccggcaaata caggaggttt acaatgaaac
    16741 gcgcgtcttt gcttacactc acgcttatcg gcgcttttag cgccatccag gctgcctggg
    16801 cggttgatta tccgctacca ccaaccggaa gccgactggt tgggcaaaat caaacgtata
    16861 cggtgcaaga aggggataaa aaccttcagg ctattgcccg acgttttgat actgcagcaa
    16921 tgttgatcct tgaagccaat aacactatcg ccccggtgcc aaaacctggt acgacgataa
    16981 ctattccttc acaactgtta ttacctgatg caccgcgtca ggggattatc gttaaccttg
    17041 cagagctgcg cctttattat tatccgccgg gagaaaatat tgtgcaggtt tatccaatag
    17101 gtattggatt gcaggggctg gaaacgccgg tgatggaaac gcgtgttggg cagaaaatcc
    17161 ctaacccaac ctggacgcct acggcaggca ttcgtcagcg ttcgctggag cgtggcatta
    17221 aattaccgcc agtcgttcct gccggaccaa ataacccgct aggacgttac gcactgcgcc
    17281 tcgcgcatgg taatggcgaa tacctcattc atggcaccag tgcgccggac agcgttggtt
    17341 tgcgcgtcag ttcagggtgt attcgcatga atgcaccgga tattaaagcc ttgttctcca
    17401 gcgtgcggac gggaacgccg gtgaaagtga tcaacgaacc ggtgaaatat tccgtggaac
    17461 ctaacgggat gcgttatgtt gaagtacatc gaccactatc agcagaagaa cagcaaaacg
    17521 ttcagacaat gccatacaca ctgccagcag gctttacgca atttaaagac aataaggctg
    17581 tagatcagaa gttagtcgat aaagcgttgt atcgtcgggc agggtatccg gtttcggtga
    17641 gcagtggagc aactcccgca gccagcaatg cgccttcagt agagtcagcg cagaatggtg
    17701 aaccagagca agggaatatg ttacgcgcga cgcagtagtg gtaaatagca gacaaaaaaa
    17761 tggcgcacaa tgtgcgccat ttttcacttc acaggtacta ttacttgcgg tatttagtag
    17821 ccatgttgtc cagacgctgg ttagcacgag ctgcgtcatc tttagcagcc tgaacgtcgg
    17881 aacgcattgc gttcacgtcg ttgctcagct ggtcaacttt agcgttcaga gtctgaacgt
    17941 cagaagacag ctgatcgatt ttagcgttgc tggagcaacc tgccagcaga gtagaaccca
    18001 ggattaccgc gcccagtacc agtttagtag ctttcattat taataccctc tagattgagt
    18061 taatctccat gtagcgttac aagtattaca caaagttttt tatgttgaga atattttttt
    18121 gatgggaagg cacttatttt tgatcgttcg ctcaaagaag catcgaacgc tgggttttag
    18181 ataaaaaaat tgagagaaaa caggatgctt ccatcggat
//
LOCUS       DAEBXN010000084        18046 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.084, whole genome shotgun sequence.
ACCESSION   DAEBXN010000084 DAEBXN010000000
VERSION     DAEBXN010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18046
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.084"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            441..1124
                     /locus_tag="KJL99_002922"
     CDS             441..1124
                     /locus_tag="KJL99_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HBD4437581.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IVLLEKQQAQLKSQLETIQKQQTGIINSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            1168..5830
                     /locus_tag="KJL99_002923"
                     /pseudo
     CDS             1168..5830
                     /locus_tag="KJL99_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
     gene            6198..7853
                     /gene="lldP"
                     /locus_tag="KJL99_002924"
     CDS             6198..7853
                     /gene="lldP"
                     /locus_tag="KJL99_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HBD4437582.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLNKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7853..8629
                     /gene="lldR"
                     /locus_tag="KJL99_002925"
     CDS             7853..8629
                     /gene="lldR"
                     /locus_tag="KJL99_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HBD4437583.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8626..9816
                     /gene="lldD"
                     /locus_tag="KJL99_002926"
     CDS             8626..9816
                     /gene="lldD"
                     /locus_tag="KJL99_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HBD4437584.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            10012..10485
                     /gene="trmL"
                     /locus_tag="KJL99_002927"
     CDS             10012..10485
                     /gene="trmL"
                     /locus_tag="KJL99_002927"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HBD4437585.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10538..11359)
                     /gene="cysE"
                     /locus_tag="KJL99_002928"
     CDS             complement(10538..11359)
                     /gene="cysE"
                     /locus_tag="KJL99_002928"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HBD4437586.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11439..12458)
                     /gene="gpsA"
                     /locus_tag="KJL99_002929"
     CDS             complement(11439..12458)
                     /gene="gpsA"
                     /locus_tag="KJL99_002929"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBD4437587.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12458..12925)
                     /gene="secB"
                     /locus_tag="KJL99_002930"
     CDS             complement(12458..12925)
                     /gene="secB"
                     /locus_tag="KJL99_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HBD4437588.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12988..13239)
                     /gene="grxC"
                     /locus_tag="KJL99_002931"
     CDS             complement(12988..13239)
                     /gene="grxC"
                     /locus_tag="KJL99_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HBD4437589.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGCTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13381..13812)
                     /locus_tag="KJL99_002932"
     CDS             complement(13381..13812)
                     /locus_tag="KJL99_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBD4437590.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKSVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            14057..15601
                     /gene="gpmM"
                     /locus_tag="KJL99_002933"
                     /gene_synonym="pgmI"
     CDS             14057..15601
                     /gene="gpmM"
                     /locus_tag="KJL99_002933"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HBD4437591.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLAGA
                     VHKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15611..16894
                     /gene="envC"
                     /locus_tag="KJL99_002934"
     CDS             15611..16894
                     /gene="envC"
                     /locus_tag="KJL99_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HBD4437592.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVTMQHAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRERQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16898..17857
                     /locus_tag="KJL99_002935"
     CDS             16898..17857
                     /locus_tag="KJL99_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HBD4437593.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(17844..18046)
                     /locus_tag="KJL99_002936"
                     /pseudo
     CDS             complement(17844..18046)
                     /locus_tag="KJL99_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT         4545 a         4663 c         4674 g         4164 t
ORIGIN      
        1 actcccttta cttcgattta ccgctggttg atattctgaa aatgcatttt taccgaaata
       61 gccatctata aacaataaaa atatatagaa gatatcattt gttaattaac aagcatttac
      121 gtctcttttt ttcggattta attgtgaaat tttaatcatc atattcacaa aatcaattat
      181 ctgtgaaatt tttcccagac atggtttctt tcatatttga catttattaa taatcatcat
      241 tagaatccac gaaaaataat atattcatac aaacacagac atcttttgaa acgaatgata
      301 catttttatg atgaatgctt aaaacaatat ttaatcgaca cacatcactg tcattaatgt
      361 aaagcaacat agctcaccaa taatacaatg gacaactcta ccggcattga gcgctggtcg
      421 gaggaaataa ggatttttac atgaacttga agaagatttt tttcagtgct gtcactgttt
      481 cagttctctg cgcattaacg ggatgtgact acattgaaga aggcaagcca gaaagtagtt
      541 tactgaaaca acaagaagaa cataataata aaattgtgct acttgaaaaa caacaagcac
      601 agctaaagag tcaactcgaa actattcaaa aacaacaaac tggcataata aacagtacaa
      661 agactttaac tcacgtaatt aaatccgtta aagaccaaca aaatactttt atttttaccg
      721 agtttaaccc agcaaaaacg aagtatttca tcctgaacaa cggttcggtg gctttagcgg
      781 gtcgagtgtt atccattgac gccacagaga atggtagtgt tattcatatc tcactggtca
      841 acttattaag tacacctatc tcaaacattg gttttaatgc gacatggggt ggtgaaaaac
      901 ctgtcgatgc caaagagttt gcccggtggc aacaattact tttcaacaca tcaatgaaat
      961 ccacattgaa attattacca ggtcaatggc aagacattaa tttgaccctg aagggtgtat
     1021 cgcccaataa cctgggatat ctgaagttag ccatcaacat ggaaaatatt cagttcgaca
     1081 atcttccctc tgctgaaaat cggcagaaaa gaagcaaaaa ataaatgcag ttagtacgct
     1141 tataacaagg aatagcgagc atcattaatg aacaaaatat ttaaagttat ctggaaccct
     1201 gcgacaggga attatactgt taccagcgaa acggcaaaaa gccgtggcaa gaaatctggg
     1261 cgcagtaagc tgttaatttc tgcgctggtt gcgggtggaa tgttgtcgtc gtttggggca
     1321 ttggcgaatg ccgggaatga caacggtcag ggtgttgatt acggtagtgg atcagctggc
     1381 gacggctggg ttgctatagg caaaggggcg aaagcaaata cttttatgaa caccagtggt
     1441 tccagtactg ctgtgggtta tgacgctata gctgaaggcc aatatagctc tgccatcggg
     1501 tcaaaaaccc atgcgattgg tggtgcatca atggcctttg gggttagtgc aatatcagaa
     1561 ggcgatagaa gtatagcact gggtgcctct tcgtattcat tgggccaata ctcaatggcc
     1621 ctcggccgtt attcaaaagc attgggtaaa ttgtctattg ctatggggga ctcttccaaa
     1681 gcggaaggag caaacgccat tgccctgggg cgtagcagtg tagctagcgg tactgacagc
     1741 ctcgcatttg gcagacaatc acttgccagc gcagcgaacg ctattgcgat aggtgctgag
     1801 accgaagccg ctgaaaatgc aactgctatt ggcaataatg cgaaggcaaa agggactaat
     1861 agcatggcaa tggggttcgg aagccttgcc gataaagtca atactatcgc attaggaaat
     1921 ggcagccagg ctctggcaga taatgcaatc gccataggcc agggcaacaa agctgatggc
     1981 gtggatgcca tcgctctggg taatggtagc cagtcgagag gcttaaacac cattgcctta
     2041 ggcacagcca gtaatgcaac tggtgataag agtcttgcgc ttggtagtaa tagcagtgcc
     2101 aacggtatta actctgtcgc gctgggcgca gattccattg cggatttaga caataccgtc
     2161 tctgtcggca atagttcatt aaaacgcaag atcgttaatg tgaaaaatgg cgcgatcaag
     2221 tctgacagtt acgatgccat taatggttca cagctttatg ccattagcga ctcggtagca
     2281 aaaaaggctt ggaggagggg ctgcagtaga tgttgatgac ggtactgtta cagcaccaac
     2341 ctacaattta aaaaatggta gcaaaaataa cgtaggggct gcgctcgctg tacttgatga
     2401 aaacaccctg caatgggacc aaaccaaagg caaatacagc gctgctcatg gtactagtag
     2461 cccaactgcc agcgtaatca ccgatgttgc ggatggcacg atttcagcct ccagtaagga
     2521 tgcggttaac ggttcccaac tgaaagctac caatgacgat gtcgaagcca acaccaccaa
     2581 tatcaccaac ctgacggatt ccgttggtga ccttcaggct gatgccctgc tctggaacga
     2641 aactaaaaag gcattcagtg cagctcacgg ccaggatacc accagcaaaa tcaccaacgt
     2701 taaagatgcc gacctgacgg ctgacagcac tgatgctgtt aacggctctc agctgaaaac
     2761 caccaacgat gctgtggcga cgaataccac caatatcgcc aataacactt ccaatattgc
     2821 cactaacacc accaacatct ctaacctgac tgagacggtg actaatcttg gtgaggatgc
     2881 gctgaaatgg gataaggaca atggtgtatt cacggcagct catggcaccg agaccaccag
     2941 caaaataacc aacgttaaag atggcgacct gacgactggc agcaccgatg ccgttaacgg
     3001 ctctcagctg aaaaccacca acgatgccgt ggcgacgaat accaccaata tcgccactaa
     3061 caccaccaac atctctaatc tgactgagac ggtgactaat cttggtgagg atgcgctgaa
     3121 atgggataag gacaatggtg tcttcactgc agctcatggc aacaataccg ccagcaaaat
     3181 caccaatatc ctggacggca cagtcactgc aaccagttcc gatgccatta acggtagcca
     3241 gctttatgac ttaagcagca atatcgccac ctacttcggc ggcaatgctt ctgtgaatac
     3301 tgacggtgtg tttaccggtc caacctacaa aatcggtgaa acaaattatt ataacgtcgg
     3361 cgatgcactg gctgcgatta actcctcatt tagcacgtct ctcggcgatg ctctgctttg
     3421 ggatgccacc gcaggtaaat tcagtgccaa acacggtact aatggtgacg caagcgtgat
     3481 cactgatgtc gcagatggtg aaatttcaga ctccagttct gacgcagtaa acggctcaca
     3541 actccacggc gtgagcagtt atgttgttga tgcgctgggg ggtggtgccg aagtcaatgc
     3601 agacggcacc atcactgcgc cgacgtacac cattgctaat gctgattacg ataatgtcgg
     3661 tgatgccctg aatgctatcg ataccactcc tgacgacgct ctgctctggg atgcggacgc
     3721 cggtgaaaat ggtgcattta gcgccgctca cggaaaagat aaaactgcca gtgtaatcac
     3781 taacgtcgct aacggtgcaa tctctgctgc cagcagcgac gcgattaacg gctcacaact
     3841 ctataccacc aataagtaca tcgctgatgc gctgggtggt gacgcagaag tcaacgctga
     3901 cggcaccatc accgcaccga cttacaccat tgcgaacgcc gagtacaaca acgtcggtga
     3961 cgccctggat gcgcttgatg ataacgccct gctgtgggat aagactgcca atggcggtgc
     4021 tggagcctac aatgccagcc atgacggtaa agccagcatc atcactaatg tcgctaatgg
     4081 cagtattagt gaggacagta ccgatgcagt gaacggttct cagttgaatg cgacgaatat
     4141 gatgattgag cagaacaccc aaattatcaa tcagctcgct ggtaacaccg acgcaaccta
     4201 tatcgaagaa aatggcgctg gtatcaacta cgttcgtact aacgacaacg atttagcctt
     4261 taacgatgca agcgcctctg gtgtcggcgc tacagctgta ggttataacg ctgtcgcgtc
     4321 tggtgccagc agcgtagcca ttggtcagaa cagcagcagc accgttgata ccggtattgc
     4381 gctgggtagc agctccgttt ccagccgtgt gattgccaaa ggttctcgtg acactagcgt
     4441 aacggaaaat ggcgtggtga ttggttatga caccactgac ggcgaactgc taggtgcatt
     4501 gtcaattggt gatgacggta aataccgcca aatcatcaac gtagctgatg gttcagaagc
     4561 ccatgacgcc gttacggttc gccagttgca gaacgctatt ggagcggtcg ccactacgcc
     4621 aaccaagtac tttcacgcca actcaacggc agaagactca ctggccgttg gtgaagactc
     4681 gctggcaatg ggtgcgaaaa ctgtcgttaa tggtaatgca ggtattggta tcggtttgaa
     4741 cactctggtt ctggctgatg cgatcaacgg cattgctatc ggcagtaacg cacgcgcaaa
     4801 ccatgcaaac agtatcgcaa tgggtaacgg ttctcagacc acccgtggtg cacagactgg
     4861 ctacaccgcc tacaacatgg acgcaccgca gaactctgta ggtgagtttt ctgtcggtag
     4921 tgaagacggt caacgtcaga tcacaaacgt cgcagctggt tcagcggata ccgatgcggt
     4981 taacgtgggt cagttgaaag tcactgatga gcgcgtagcg caaaataccc agagcattac
     5041 taacctgaac aatcaggtca ctaatctgga tactcgcgtt actaatatcg aaaacggtat
     5101 tggcgacatt gtcaccaccg gtagcaccaa gtacttcaag accaacaccg atggcgtaga
     5161 tgccaacgcc cagggtaaag atagcgttgc tattggttct ggttccattg ctgccgctga
     5221 caacagcgtc gcactgggta ccggttccgt tgcagaggaa gaaaatacaa tctctgtagg
     5281 ttcttctact aaccaacgcc gtatcaccaa cgtagctgcc agtgttaatg ccaccgatgc
     5341 ggttaacgtg tcgcagctga aatcttctga agcaggcgga gtgcgctacg acaccaaagc
     5401 tgatggttct atcgactata gcaatatcac cctcggtggt ggcaacggca gtacgactcg
     5461 tatcagcaac gtctccgctg gcgtcaacaa caacgacgcg gtgaactacg cgcagttgaa
     5521 gcaaagcgcg caggaaacga agcaatacac cgatcagcgg atggttgaga tggataacaa
     5581 actgtctaaa actgaaagca agttgagcgg tggtatcgct tctgcaatgg caatgaccgg
     5641 tctgccgcag gcttatacac cgggtgccag catggcttct attggtggcg gtacttacaa
     5701 cggtgaatcg gcagttgctt taggtgtatc gatggtgagc gccaatggtc gttgggtcta
     5761 caaattacaa ggtagtacca atagccaggg tgaatactcc gccgcactcg gtgccggtat
     5821 tcagtggtaa tgatccatta agttagtgtg actaagggca aacgccaaat tgcctgatgc
     5881 gttacgcgca tcaggcaaga taaggcgctc cgggtcaaca atctaagggc aattctctga
     5941 tgaggattgc ccttttcttt accagacatc tccccccaca agaattggcc ctaccaattc
     6001 ttcgcttatc tgacctctgg ttcacaattc cccaattaaa actcacatca atattgccaa
     6061 tacataacat ttagttaacc attcattgtc attatcccta cacaacacaa ttggcagtgc
     6121 cacttttaca caacgtgtga caaggagatg agcaacagac tcattacacg atgtgcgtgg
     6181 actctaggag acctgcaatg aatctctggc aacaaaacta cgatcccgcc gggaatatct
     6241 ggctttccag tctgatagca tcgcttccca tcctgttttt cttctttgcg ctgattaagc
     6301 tcaaactgaa aggatacgtc gccgcctcat ggacggtagc aatcgccctt gccgtggctt
     6361 tgctgttcta taaaatgccg gtcgctaacg cgctggcctc ggtggtttat ggcttcttct
     6421 atggtttgtg gcccatcgcg tggatcatta ttgcagcagt gttcgtctat aagatctcgg
     6481 tgaaaaccgg gcagtttgac atcattcgct cgtctattct ttcgataacc cctgaccagc
     6541 gtctgcaaat gctgatcgtc ggtttctgtt tcggcgcttt ccttgaagga gccgcaggct
     6601 ttggcgcacc ggtagcaatt accgccgcat tgctggtcgg cctgggtttt aaaccgctgt
     6661 acgccgccgg actgtgcctg attgttaaca ccgcgccagt ggcatttggc gcgatgggca
     6721 ttccaatcct ggttgccgga caagtaacag gcatcgacag ctttgagatt ggccagatgg
     6781 tggggcgtca gctaccgttt atgaccatta tcgtgctgtt ctggatcatg gcgattatgg
     6841 acggctggcg cggtatcaaa gagacgtggc ctgcggtcgt ggttgcaggc ggctcgtttg
     6901 ccatcgctca gtaccttagc tctaacttca ttgggccgga gctgccggac attatctctt
     6961 cgctggtatc actgctctgc ctgacgctgt tcctcaaacg ctggcaacca gttcgcgtat
     7021 tccgcttcgg tgacttgggg gcgtcacagg ttgatatgac gctggcccac accggttaca
     7081 ccgcgggtca ggtgttacgt gcctggacac cgttcctgtt cctgaccgct accgtaacac
     7141 tgtggagcat cccgccgttt aaagccctgt tcgcatcggg tggtgcgctg tatgagtggg
     7201 tgatcaacat tccggtgccg tacctcaata aactggttgc ccgtatgcca ccagtggtca
     7261 gcgaggctac agcctatgcc gccgtgttta agtttgactg gttctctgcc accggcaccg
     7321 ccattctgtt tgctgcactg ctctcgattg tctggctgaa gatgaaaccg tctgacgcta
     7381 tcagcacctt cggcagcacg ctgaaagaac tggctctacc catctactcc atcggcatgg
     7441 tgctggcgtt cgcctttatc tcgaactatt ccggactgtc atcaacactg gcactggcac
     7501 tggcgcacac cggtcatgca ttcaccttct tctcgccgtt cctcggctgg ctgggggtat
     7561 tcctgaccgg gtcggatacc tcatctaacg ccctgttcgc cgcgctgcaa gccaccgcag
     7621 cacaacaaat tggcgtctct gatctgttgc tggttgccgc caataccacc ggtggcgtca
     7681 ccggtaagat gatctccccg caatctatcg ctatcgcctg tgcggcggta ggcctggtgg
     7741 gcaaagagtc tgatttgttc cgctttactg tcaaacacag cctgatcttc acctgtatgg
     7801 tgggcgtgat caccacgctt caggcttatg tcttaacgtg gatgattcct taatgattgt
     7861 tttacccaga cgcctgtcag acgaggttgc cgatcgtgtg cgggcgctga ttgatgaaaa
     7921 aaacctggaa gcgggcatga agttgcccgc tgagcgccaa ctggcgatgc aactcggcgt
     7981 atcacgtaat tcactacgcg aggcgctggc aaaactggtg agcgaaggcg tgctgctcag
     8041 tcgacgcggc ggcgggacgt ttattcgctg gcgtcatgac acatggtcgg agcaaaacat
     8101 cgtccagccg ctaaaaacgc tgatggccga tgatccggat tacagtttcg atattctgga
     8161 agcccgctac gccattgaag ccagcaccgc atggcatgcg gcaatgcgcg ccacacctgg
     8221 cgacaaagaa aagattcagc tttgctttga agcaacgcta agtgaagacc cggatatcgc
     8281 ctcacaagcg gacgttcgtt ttcatctggc gattgccgaa gcctcacata acatcgtgct
     8341 gctgcaaacc atgcgcggtt tcttcgatgt cctgcaatcc tcagtgaagc atagccgtca
     8401 gcggatgtat ctggtgccac cggttttttc acaactgacc gaacaacatc aggctgtcat
     8461 tgacgccatt tttgctggtg atgccgacgg ggcgcgtaaa gcaatgatgg cgcaccttag
     8521 ttttgttcac accaccatga aacgattcga tgaagatcag gctcgccacg cacggattac
     8581 ccgcctgccc ggtgagcata atgagcattc gagggagaaa aacgcatgat tatttccgca
     8641 gccagcgatt atcgcgccgc agcgcaacgc attctgccgc cgttcctgtt ccactatatg
     8701 gatggtggtg catattctga atacacgctg cgccgcaacg tggaagattt gtcagaagtg
     8761 gcactgcgcc agcgtattct gaaaaacatg tccgacttaa gcctggaaac gacgctgttt
     8821 aatgagaaat tgtcgatgcc ggtagcactg gcaccggtgg gtttgtgtgg catgtatgcg
     8881 cgtcgtggcg aagttcaggc agccaaagcg gcggacgcgc atggcattcc gtttactctc
     8941 tcgacggttt ccgtttgccc gattgaagaa gtcgcgccag ccatcaagcg cccaatgtgg
     9001 ttccagcttt atgtactgcg cgatcgcggc tttatgcgta acgcgctgga gcgagcaaaa
     9061 gcagcgggtt gttcgacgct ggttttcacc gtggatatgc cgacaccggg cgcacgctac
     9121 cgtgatgcgc attcaggtat gagcggccca aatgcggcaa tgcgccgcta cttgcaggcg
     9181 gtgacgcatc cgcaatgggc gtgggatgtg ggcctgaacg gtcgtccaca tgatttaggt
     9241 aatatctcag cttatctcgg caaaccgacc ggactggaag attacatcgg ctggctgggg
     9301 aataacttcg atccgtccat ctcatggaaa gaccttgagt ggatccgcga tttctgggat
     9361 ggcccgatgg tgatcaaagg gatcctcgat ccggaagatg cgcgcgatgc agtacgtttt
     9421 ggtgctgatg gaattgtggt ttctaaccac ggtggccgcc agctggacgg tgtactctct
     9481 tccgctcgtg cgttgcccgc tattgcagat gcggtgaaag gtgatatcgc cattctggcg
     9541 gatagcggaa ttcgtaacgg gcttgatgtc gtgcgtatga ttgcgctcgg tgccgacacc
     9601 gtactgctgg gtcgtgcttt cctgtatgcg ctggcaacag cgggccaggc gggtgtagct
     9661 aacctgctaa atctgatcga aaaagagatg aaagtggcga tgacgctgac tggcgcgaaa
     9721 tcgatcagtg aaattacgca agattcgctg gtgcaggggc tgggtaaaga gttgcctacg
     9781 gcactggctc cgatggcgaa agggaatgcg gcatagtcgt ttgcgccccc tcaccctaac
     9841 cctctccctc agggagaggg gaccgttcgg cgctgtatgt actccctcac tctgaaacga
     9901 caccgcactc tttttttctc cctcgcccct ccggggaggg ccggggtgag gggaaaaggc
     9961 cgcacggttc accaattttc tgctatcctg cccccgcact aagggggcag tatgctaaac
    10021 atcgtacttt acgaaccaga aattccgcca aatactggca acatcatccg tctttgcgct
    10081 aataccggct ttcgtctaca tatcatcgaa ccgatgggat ttgcctggga cgacaagcgc
    10141 ctgcgccgcg cggggctgga ctatcacgag tttaccgccg ttacgcgtca tcatgactat
    10201 cgcgcgttcc tcgaagcaga aaatccccag cgcctgtttg ccctcaccac gaaaggtacg
    10261 cctgctcaca gcgccgtaag ctatcaggat ggcgattatc tgatgttcgg cccggaaaca
    10321 cgcggcctgc cagcgagcat tcttgatgcc ctgcccgctg aacaaaaaat tcgcattccg
    10381 atggtgccgg acagccgcag catgaatctg tccaatgcgg tgtcggtagt ggtgtatgaa
    10441 gcctggcggc agctgggata tccgggagcg ttgttgagag attagttact gtatgccgga
    10501 tagatgatta catcgcatcc ggcacgatca caggacatta gatcccatcc ccatactcaa
    10561 atgtatggtt aataccgttg aaatgctggt ccatatccat tgatggctta tcgctgtctg
    10621 gtttaccgac aatacgagcc ggaacgccag cggcggttgt atgcggcggc accggttgca
    10681 gcaccacgga acctgcgcca atcttcgcgc cgcgaccaac ttcaatattg ccgaggattt
    10741 tcgcgcccgc gccaatcatc acaccttcac gaattttcgg gtgacggtca ccaccagatt
    10801 tacccgtacc gccaagcgtc acagattgca gaatcgatac gtcgttttca attaccgccg
    10861 tttcacccac aacgatgccc gtggcgtggt cgagcataat gccacggcca atttttgctg
    10921 ccgggtgaat atcgacctgg aacgtcacag aaacctggtt ttgcagaaag attgccaatg
    10981 cgcgacgccc ctgattccac aaccagtgac cgatgcgata ggcctgcaag gcatgaaaac
    11041 ccttcaggta taacaacggg gttgagtatt tatcgactgc cgggtcgcgg gtacgcaccg
    11101 cctgaatatc acaggccgca gaggcgatca tttccgggtc agcggcgtag gcttcttcaa
    11161 ccacttcacg gatagcaata gcaggcataa ttggcgatga cagcttgttc gccagcatgt
    11221 agctcagtgc actgccaagg ttttcgtgct tgagtagcgt cgcgtggtaa aaactggcca
    11281 gcattggctc acagtccgcc agcgttctgg cttcggcttt aatattgttc cagacaattt
    11341 ccagttcttc acacgacatt gcttactcca cacgatgaga taatgaccgg cccgttctgc
    11401 gcgggccggg tcatagcggt aacaaaggtt ccctggggtt agtgactgct gcgctcgtcc
    11461 ttgcgtgcac gacctaataa tgtcaatgct gcctcgcgcg cgttttttcc gcaatataat
    11521 acttgataaa tttcctcggt tattggcatt tcaacgccga agcgatgcgc cagttcgcgg
    11581 acttctttcg tattgcggta gccttccacc acctgaccaa tcttctcctg cgcgctttgt
    11641 acatccatgc cctgaccgag catcatacca aaacggcggt tacgcgactg gttgtcggta
    11701 caggtaagca ccagatcgcc cagccccgcc atgcccataa aggtggcagg atcggcaccc
    11761 agcgccgcac caagacgcga catttcagcc agcccacggg tgatcagcgc cgtacgcgca
    11821 ttcgcaccaa aaccgatacc gtcagacatc cccgcgccaa tggcaatgac gtttttcacc
    11881 gcgccgccaa gctgcacgcc aatgaaatcc gggttgctgt aaacgcggaa acttttgccg
    11941 cagtgcagca gctgctggag atcatcggca aaggtctgat cggtcgaggc cagcgaaata
    12001 gctgtcggta aacctgccgc cagttctttc gcaaacgttg ggccagagat aaccgccagc
    12061 ggaatttgat cgcctaaggc ctcacgcgcc acgtcctgta acagacgtcc ggtttccgct
    12121 tccagccctt tggtcgccca caccagacgc gcatcaggac gcatcagcgg tttaatctgg
    12181 cgcagcactt caccaaagac atggctgggt acgacgacga gaatattacg actggctgcc
    12241 agcgcagtgg cgagatcgct ttcaagatgg agcgtatcgg gaaaaggcac atcggggaga
    12301 aacgcggcgt tacagcggtc gcgttcaagc gttgcgatat gttcagggtc atggccccag
    12361 aggacaacct cgtggccatt tcttgccagg gtaatggcaa gagcggtgcc gtacgagccg
    12421 gcaccgatca cagtcattga agcattacgt tggttcatca ggcatcctga tgttcttcag
    12481 taccttcgcc agcctgctgc tgcaaatagt tcatgaacag cgcatcgaag ttaaccggcg
    12541 caaggttcag ttgcgggaat gtaccgcggg ataccatgct ggtgatgcac tcacgagcat
    12601 acgggaacag aatgttcggg cagtatgcgc ccaggcaatg tgccatctgg gtgccttcga
    12661 tacccgcgat ggagaaaata ccgccctgct gaacttcaca caggaacgcg gtttcttcgc
    12721 ccaaggaggc cgttacggta acacgcagta ccacttcgta tacgtcatct gccagttggg
    12781 aagatgccgt atccagatca agtttaactt ctggttgcca atctttctgg aaaacgtgcg
    12841 gcgcgttcgg cgcttcgaaa gagatatcct tggtataaat acgttggatc tggaaagtca
    12901 tttcagtgtt gttttgttct gacatgtgta gaaaaccctt aagtgttgtc cttaaatact
    12961 gcgcaatgta ataccaacgt tcacacgtta tttcagcagg ggatccagtc caccacgtgc
    13021 atccaatgca tacaagtcat cacagccgcc aatgtgctgt gcgtcaataa aaatctgggg
    13081 aaccgtggtg caaccgctgc gtttgatcat ctcttcacgc ttggcggcat tgccatcgat
    13141 cggcaactcc tggaaactca cgcccttgct gctcagcagt gcttttgcac gatggcaata
    13201 cgggcaggtt tctttggtat agatttcaac attggccata atttagctcc tgttttttta
    13261 ccctgaaatt tcatgttgca gggcggctgc aaaatggagt gtccccagaa gcttataaca
    13321 ataagtcgct gggggcctaa ttgcagctaa cggcctgcat catgaaagac gacaggtaaa
    13381 ttatttgccg cgcaccagag gcaagttttc gccagcccag ccagcaacgc cctctttcag
    13441 cacgaatact tgcgcaaaac cagctttcgt cagcgcgttt gcaggctcct ggcactgcat
    13501 gccagaaccg tctaccacga taaccgattt gtctttgtgc ttctcaagct caccaacatt
    13561 gttggctttg atttcgctcg gcaacagatt gatagaacct gcgatatggc ctttacggaa
    13621 gtcatcacgc tgacgtaaat ccacaaccac agcgtcttct ttgttgatca gacgcgtagc
    13681 ttcaccacga gtaatcacct tcactttcga ggtcaggctc ttaaacgtag tcacaagaac
    13741 cgccaccagt aacgcgatcc aggcgatact cagtatggga tgacggccaa caaattgcat
    13801 aatttcttgc atggggggta acaactcccg acgtagtgat taaaaaaacc aggaaaggag
    13861 tatacctgcg cggtgcggca aatacagcca gcgcgttaac tggaatgcaa ttttgcgggg
    13921 cacgacgaaa aaaaagcgca aatctggcat cggcttgccg cgagcggtcg tatattttga
    13981 tcttcagagg ctattttatc gattcagctg tagtaaaatt acgcaaattt tgactcttga
    14041 gtatgaggtt gtcgcaatgt cggtttctaa aaaacctatg gtactggtga ttctggatgg
    14101 ctatggctat cgtgaagaac agcaggataa cgccattttt agtgctaaaa ccccggtaat
    14161 ggatgcactg tgggccaatc gtccgcatac cctgatcgac gcttccggtc tggaagtcgg
    14221 tctgcctgac cgtcagatgg gtaactccga agtaggccac gttaacctgg gtgccggccg
    14281 catcgtgtat caggacctga ctcgtctgga cgttgaaatc aaagatcgcg ctttctttgc
    14341 taatccagtg ctggctggcg cggtacataa agcgaaaaac gcaggtaaag cagtacacat
    14401 tatgggtctg ctctccgcag gcggcgtaca cagccacgaa gatcacatca tggcgatggt
    14461 agaactggca gctgaacgcg gcgcagaaaa aatctacctg cacgcattcc ttgacggtcg
    14521 cgacaccccg ccgcgcagtg ctgaatcctc gctgaaaaaa tttgaagaga aatttgctgc
    14581 gctgggtaaa ggccgcgtag cgtccatcat tggtcgttac tacgcgatgg accgcgataa
    14641 ccgttgggat cgcgttgaaa aagcctatga cctgctgact ctggcgcagg gtgagttcca
    14701 ggccgatact gccgttgctg gtttgcaggc agcttatgct cgcgacgaaa acgacgagtt
    14761 cgtgaaagcg accgttattc gtgcagaagg tcagccagat gcggcaatgg aagacggcga
    14821 tgcgctgatt ttcatgaact tccgtgctga ccgcgcgcgt gaaatcactc gtgcattcgt
    14881 gaacgctgat ttcgatggct tcgcgcgtaa gaaagtggtt aacgtcgatt tcgtgatgct
    14941 gaccgaatac gccgctgaca tcaaaactgc ggttgcttac ccacccgctt ccctggttaa
    15001 caccttcggc gagtggatgg cgaaaaacga caaaactcag ttgcgtattt ccgaaaccga
    15061 aaaatatgcc cacgttactt tcttcttcaa cggtggcgta gaagagtcgt tcaaaggcga
    15121 agatcgcatt ctgatcaact caccgaaagt ggctacctac gatctgcaac cggaaatgag
    15181 ctccgcagag ctgaccgaaa aactggttgc ggccatcaag agcggcaaat acgacaccat
    15241 tatctgtaac tatccgaacg gcgacatggt aggtcacacc ggggtgatgg aagcggcggt
    15301 taaagcggtt gaagcactgg atcactgcgt ggaagaagtc gcgaaagcgg ttgagtccgt
    15361 gggtggacaa ctgctgatca ccgctgacca cggtaacgct gaacagatgc gcgatccggc
    15421 aacgggtcag gcacacacgg cacacaccaa cctgccagtt ccgctgattt acgttggtga
    15481 taagaacgtg aaagcggttg caggcggcaa actttctgac atcgcgccga ccatgttgtc
    15541 gctgatgggt atggaaatcc cgcaagagat gactggtaag ccgctgttca tcgtggaata
    15601 atccctcccc atgaggggaa aggcgattaa taccatgaca cgggccgtga aaccgcgcag
    15661 gtttgcaatc aggcccatca tctacgccag cgttcttagc gctggcgtat tgttgtgcgc
    15721 cttttccgcc cacgcggatg agcgtgacca actcaaatct attcaggccg atatcgccgc
    15781 aaaagagcgc gcggtacgcc aaaagcaaca acaacgcgca agcctgctcg cacaattgaa
    15841 aaagcaggaa gaagcgatct ctgaagccac ccgtaagctg cgcgaaacgc aaaacacgct
    15901 caatcaactc aataaacaga ttgatgagat gaacgcgtcg attgccaaac tggagcagca
    15961 aaaagccgcc caggagcgca gcctcgccgc acaactggat gccgcattcc gtcagggcga
    16021 gcataccggt attcagctaa ttctcagcgg tgaagaaagc cagcgtggac agcgtttaca
    16081 ggcttatttc ggctatctca accaggcgcg acaagaaacc attgcccagt tgaagcaaac
    16141 gcgtgaagaa gtcaccatgc agcatgctga actggaagag aaacaaagcg agcaacaaac
    16201 gctgttatat gaacagcgcg cccaacaggc gaaactgact caggctctga acgagcgtaa
    16261 aaagacgctg gcggggctgg aatcttccat ccagcaaggt cagcagcagc taagtgaact
    16321 gcgtgccaac gaatcccgtc tgcgtaacag cattgcccgt gctgaagccg cggcgaaagc
    16381 gcgtgctgaa cgagaagcgc gtgaggccca ggcggttcgc gagcgccaga aagaagcgac
    16441 gcgcaaaggc accacctaca aaccgaccga aagcgaaaaa tcgctgatgt cccgaactgg
    16501 tggcctgggt gcgccgcgtg gtcaggcatt ctggccggtt cgcgggccga cgctgcatcg
    16561 ctatggcgag cagctacagg gtgaattacg ctggaaaggg atggttatcg gtgcctctga
    16621 aggtactgaa gttaaagcaa ttgccgacgg tcgagtgatt ctggctgact ggctgcaagg
    16681 ttacggtctg gtggtggtgg ttgagcacgg taaaggcgac atgagtcttt acggctataa
    16741 tcagagcgca ctggtgagcg ttggttcgca ggttcgcgcg ggccagccaa ttgcactggt
    16801 gggcagcagt ggcggtcagg gtcggccttc actctatttc gaaattcgcc gccagggtca
    16861 ggcggtcaat ccacagccgt ggttgggaag ataagttttg tttccatttc gtcgtaacgt
    16921 tcttgcattt gccgctctgt tggcgctctc ctcccccgta cttgctggca aacttgccat
    16981 cgtcattgat gattttgggt atcgcccgca caacgaaaac caggtgctgg cgatgccttc
    17041 cgctatctcc gtcgctgtat tacccgattc accgcacgcc agagagatgg cgaccaaagc
    17101 gcataacagc ggtcacgaag tgttgattca tctcccgatg gcaccgttga gtaaacagcc
    17161 gctggagaaa aatacgctac gcccggagat gagcagcgac gaaattgagc gcattattcg
    17221 tagtgcggtc aataacgtgc cctatgccgt ggggatcaac aaccacatgg gcagcaagat
    17281 gacctctaac ctgtttggta tgcagaaagt gatgcaggcg ctggagcgtt acaatcttta
    17341 cttcctcgac agcgtaacca tcggtaatac ccaggcgatg cgcgccgcgc aaggtactgg
    17401 cgtgaaggtg atcaaacgga aggtgttcct cgacgattcg cagaacgaag cggacatccg
    17461 tgtgcaattt aatcgcgcaa ttgatctggc gcgtcgcaac ggttcaacca ttgccattgg
    17521 gcatcctcac ccttcaacgg tacgcgtgct acaacagatg gtttataacc tgccgccaga
    17581 cattacgctg gtgaaagcca gcagcttgct gaatgaaccg caggttgata cttctacacc
    17641 gccgaaaaac gctgtgcctg atgcaccgcg taatccgttc cgtggcgtga agctgtgcaa
    17701 accgaagaaa ccgatagaac ctgtttatgc taatcgcttc tttgaagtat taagcgaaag
    17761 catcagccag agcacgctga tcgtttactt ccagcatcag tggcaaggct ggggcaaaca
    17821 gcccgaagcg gcgaagttta acgctagcgc aaattaaagg cgctacgcgt aatgcgatgg
    17881 tgcgaccgcg ttttgtcgcg ccattgccat aagcggaaag accacagtaa cgcctggtaa
    17941 ccgaccttca cgctgcgcac attggtaatc aggcgcttat acatacccgg aagtaaatat
    18001 ctcggcaatc atccgttggc gggtaagaat atccggctct ttgcgc
//
LOCUS       DAEBXN010000085        17987 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.085, whole genome shotgun sequence.
ACCESSION   DAEBXN010000085 DAEBXN010000000
VERSION     DAEBXN010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17987
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.085"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            157..681
                     /locus_tag="KJL99_002937"
     CDS             157..681
                     /locus_tag="KJL99_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBD4437594.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLTDNSVQS
                     GDVLSSLVMRVAQD"
     gene            762..1436
                     /locus_tag="KJL99_002938"
     CDS             762..1436
                     /locus_tag="KJL99_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HBD4437595.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLYIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNIKSNNANNWYLTIINDHGN
                     YISDKI"
     gene            1452..3932
                     /locus_tag="KJL99_002939"
     CDS             1452..3932
                     /locus_tag="KJL99_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBD4437596.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYSWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNNKSTYVRFNSGLNLLEWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQFLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGTMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYTLAASQAYGENHHEEKRFNIFISIPCDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQTGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            3948..4981
                     /locus_tag="KJL99_002940"
                     /pseudo
     CDS             3948..4981
                     /locus_tag="KJL99_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
     gene            complement(5063..5401)
                     /gene="rcnB"
                     /locus_tag="KJL99_002941"
     CDS             complement(5063..5401)
                     /gene="rcnB"
                     /locus_tag="KJL99_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HBD4437597.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(5620..6450)
                     /gene="rcnA"
                     /locus_tag="KJL99_002942"
                     /pseudo
     CDS             complement(5620..6450)
                     /gene="rcnA"
                     /locus_tag="KJL99_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
     gene            6572..6844
                     /gene="rcnR"
                     /locus_tag="KJL99_002943"
     CDS             6572..6844
                     /gene="rcnR"
                     /locus_tag="KJL99_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HBD4437598.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      6936..7032
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            7067..7855
                     /gene="thiM"
                     /locus_tag="KJL99_002944"
     CDS             7067..7855
                     /gene="thiM"
                     /locus_tag="KJL99_002944"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HBD4437599.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTRWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            7852..8652
                     /gene="thiD"
                     /locus_tag="KJL99_002945"
     CDS             7852..8652
                     /gene="thiD"
                     /locus_tag="KJL99_002945"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HBD4437600.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKIWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            8717..9538
                     /locus_tag="KJL99_002946"
     CDS             8717..9538
                     /locus_tag="KJL99_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HBD4437601.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGLDVSRWQERIDWQRVAKMRDNDIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTGMSCRHSLMGLKKRLK"
     gene            9586..10332
                     /locus_tag="KJL99_002947"
     CDS             9586..10332
                     /locus_tag="KJL99_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBD4437602.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(10306..11271)
                     /locus_tag="KJL99_002948"
     CDS             complement(10306..11271)
                     /locus_tag="KJL99_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HBD4437603.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     SDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFCPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(11268..12271)
                     /locus_tag="KJL99_002949"
                     /pseudo
     CDS             complement(11268..12271)
                     /locus_tag="KJL99_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
     gene            12311..13363
                     /gene="fbaB"
                     /locus_tag="KJL99_002950"
     CDS             12311..13363
                     /gene="fbaB"
                     /locus_tag="KJL99_002950"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HBD4437604.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLVSVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            13671..14525
                     /gene="gatY"
                     /locus_tag="KJL99_002951"
     CDS             13671..14525
                     /gene="gatY"
                     /locus_tag="KJL99_002951"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HBD4437605.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEVDALYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFLQSLKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            14554..15816
                     /gene="gatZ"
                     /locus_tag="KJL99_002952"
     CDS             14554..15816
                     /gene="gatZ"
                     /locus_tag="KJL99_002952"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HBD4437606.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDTIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGHALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAMPHQLIMNKIYDVLRAYRYGCAE"
     gene            15826..16278
                     /gene="gatA"
                     /locus_tag="KJL99_002953"
     CDS             15826..16278
                     /gene="gatA"
                     /locus_tag="KJL99_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HBD4437607.1"
                     /translation="MTNLFVRSGISFVDRSEVLTYIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            16309..16593
                     /gene="gatB"
                     /locus_tag="KJL99_002954"
     CDS             16309..16593
                     /gene="gatB"
                     /locus_tag="KJL99_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HBD4437608.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            16597..17952
                     /locus_tag="KJL99_002955"
     CDS             16597..17952
                     /locus_tag="KJL99_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBD4437609.1"
                     /translation="MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRMIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQPAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFTKQEKAVLA
                     E"
BASE COUNT         4692 a         4103 c         4571 g         4621 t
ORIGIN      
        1 ttaagtattt gttgtattta tattgcattg gttaatttgt tttaaatcaa taaaacaata
       61 tgtttacatt gcggcagctt ttttgatgtg agaaatttgt ttccaagaga tacagactct
      121 taacaaccaa aaataaattt tattaaggaa catattatga aacgttcaat tattgctgcc
      181 gctgtctttt cttctttttt tatgagcgct ggagtatttg ctgcagacgt tgataccgga
      241 acattaacca ttaaaggaaa tattgcagaa tctccgtgta aattcgaagc gggtggtgat
      301 tcagtaagta ttaatatgcc gactgtacca accactgtct ttgaaggtaa agctaaatat
      361 tctacctatg atgatgcagt cggtgtaacc agcagcatgt taaaaattag ctgcccgaaa
      421 gaagttgctg gtgtaaaact ctcgttgatc acaaacgaca aaataaccgg taacgataag
      481 gcgatagcca gtagcaacga taccgtaggt gataacagcg atgtcctaga tgtttctgca
      541 ccttttaaca ttgagagtta taaaacagcg gaaggtcaat atgctattcc gtttaaagca
      601 aaatacctga aactgacaga taactcagtg caatcaggtg atgtgttatc ttctctggtt
      661 atgcgtgtgg cgcaggatta ataaataaaa ttgcaagttt ctccaggtaa taaacatggc
      721 cgctatcccc atggcggctt tttaatttaa gaggcattaa gatgaaagga ttattatctt
      781 tactcatttt ttctatggtc cttcctgcac atgccggaat tgttatctac gggacgcgca
      841 ttatttaccc ggcagaaaat aaagaagtta tggtgcagtt gatgaaccag ggaaatcgtt
      901 cttcgctgct gcaggcgtgg attgatgatg gcgatacgtc gttaccacca gaaaaaattc
      961 aggttccttt catgttaacg ccaccagtgg caaaaatagg ggcaaattcc gggcagcaag
     1021 taaaaatcaa aattatgccg aataaactgc ccactaataa agaaagcatt ttttatctga
     1081 atgttctgta tattccacca aatagtccag agcaagaagg caagaatgca ctgaagtttg
     1141 cgatgcaaaa cagaattaag ttgttttacc ggccagcggg tattgctccg gtaaataaag
     1201 cgacctttaa aaagttactg gtaaatcgca gtggcaatgg attggtgata aaaaatgact
     1261 cagctaattg ggtgacgatt tcggatgtca aagctaataa tgttaaagtc aattatgaaa
     1321 ctattatgat tgccccctta gaaagtcaga gtgttaatat caaaagtaat aatgcaaata
     1381 actggtatct gaccattatc aatgaccacg gcaactatat tagtgacaaa atttaattgc
     1441 aggagctgcc tatgttgaga atgaccccac ttgcatcagc aatcgtagcg ttattgctcg
     1501 gcattgaagc ttatgcagct gaagaaacct ttgataccca ttttatgata ggtggaatga
     1561 aagaccagca ggttgcaaat attcgtcttg atgataatca acccttaccg gggcagtatg
     1621 acatcgatat ttatgtcaat aagcaatggc gcgggaaata tgagattatt gttaaagaca
     1681 acccgcaaga aacatgttta tcaagagaag ttatcaagcg gttaggcatt aatagcgata
     1741 acttcgccag cggtaagcaa tgtttaacat ttgagcaact tgttcagggt gggagctata
     1801 gctgggatat cggggttttt cgtctcgatt tcagtgtccc gcaggcttgg gtggaagaac
     1861 tggaaagtgg ctatgttcca ccggaaaact gggagcgggg tattaatgcg ttttatactt
     1921 cttattatgt gagtcagtat tacagcgact ataaagcgtc gggtaacaac aagagtacat
     1981 atgtacgttt taacagcggg ttaaatttac tggagtggca actgcattct gatgccagtt
     2041 tcagtaaaac aaataacaat ccaggggtgt ggaaaagcaa taccctgtat ctggaacgtg
     2101 gatttgccca atttctcggc acgcttcgcg tgggtgatat gtacacatca agcgatattt
     2161 ttgattctgt tcgcttcagc ggtgtgcggt tgtttcgtga tatgcagatg ttgcctaact
     2221 cgaaacaaaa ttttacgcca cgggtgcagg ggattgctca gagtaacgcg ctggtaacta
     2281 ttgaacagaa tggttttgtg gtttatcaga aagaggttcc tcctggcccg tttgcgatta
     2341 cagatttgca gttggccggt ggtggagcag atcttgatgt tagcgtgaaa gaggcggacg
     2401 gctcggtaac cacctatctg gtgccttatg cagcggtgcc aaatatgctg caacccggcg
     2461 tgtcgaaata tgattttgcg gcgggccgta gccatattga aggggcgagc aagcaaagtg
     2521 attttgtcca ggcaggttat cagtatggtt ttaataactt attgacgctg tatggcggca
     2581 cgatggttgc taataattac tatgcgttta ccctcggaac gggttggaac acgcgcattg
     2641 gcgcaatttc cgtcgatgcc acgaagtcgc atagtaaaca agacaacggc gatgtgtttg
     2701 acgggcaaag ttatcaaatt gcctacaaca aatttgtgag ccaaacgtcg acgcgttttg
     2761 gtctggcggc ctggcgttat tcgtcgcgtg attaccggac atttaacgat cacgtttggg
     2821 caaacaataa agataattat cgccgtgatg aaaacgatat ctatgacatt gccgattatt
     2881 accagaacga ttttggccgt aaaaatagct tttctgccaa tatgagccag tcattgccag
     2941 aaggctgggg ttctgtgtcc ttaagtacgt tatggcgaga ttactggggg cgtagcggca
     3001 gcagtaagga ttatcagttg agttattcca ataactggcg gcggataagc tataccctcg
     3061 cggcaagcca ggcgtatggc gagaatcatc atgaagagaa acgttttaat atttttatat
     3121 caattccctg tgattggggt gatgacgtta cgacgcctcg tcggcaaata tatatgtcta
     3181 actcaacgac gtttgatgat cagggttttg cctcaaataa tacgggatta tcaggaacag
     3241 tagggagtcg ggatcagttc aattatggtg tcaacctgag tcatcaacat cagggaaatg
     3301 aaacgacagc tggggcgaat ttgacctgga acgcgccggt tgcgacagtg aatggcagtt
     3361 atagtcagtc gagtacttat cgacagactg gagccagtgt ttcagggggc attgtcgcct
     3421 ggtcgggtgg cgttaatctg gcgaaccgtc tttccgaaac gtttgctgtg atgaatgcgc
     3481 caggaattaa agatgcttat gtcaatgggc aaaaatatcg cacaacaaac cgtaatggag
     3541 tggtggtata cgacggaatg acaccttatc gggaaaatca cctgatgctg gatgtgtcgc
     3601 aaagcgatag cgaagcagaa ttacgtggca accggaaaat tgccgcccct tatcgcggcg
     3661 cggttgtact ggttaatttt gataccgatc agcgcaagcc ctggtttata aaagcgttaa
     3721 gagcggatgg tcaaccatta acgtttggtt atgaagtcaa tgatatccat ggtcataata
     3781 ttggcgttgt cggccaggga agccagttat ttattcgcac caatgaaata ccgccatcgg
     3841 ttaatgtagc aattgataag caacaaggac tttcatgcac aatcaccttc ggtaaagaga
     3901 ttgatgaaag tagaaattat atttgccagt aatagtcagg tggttctatg gaaattcgca
     3961 taatgctatt tatattaatg atgatggtta tgcctgtgag ctatgcggca tgttatagtg
     4021 agttatctgt tcagcacaat ttggttgttc aggggaattt tgcacttact caaacacaaa
     4081 tagcgacata tgagcataat tttaatgatt cgtcatgcgt gagtacaaat actatcaccc
     4141 ctatgagccc atcggatatt attgttggac tttataacga taccattaaa ttaaatttac
     4201 attttgaatg gaccaataaa aacaacatca cgttgtcaaa taatcagagc agtttcacca
     4261 gtggttattc ggtaacggtg acacctgcgg ccagtaatgc aaaagtgaat atttctgcgg
     4321 ggggcggtgg ttcagtgatg attaatggtg ttgcgacatt atccagtgct tcatcatcga
     4381 cacgcgggag tgccgcagta caatttctac tgtgtttatt aggtggcaag tcatggggtg
     4441 catgtgtaaa tagctacaga aatgcattgg cacaaaatgc aggtgtctat tccttcaatc
     4501 tgacattgtc atacaacccg ataaccacaa cctgcaaaca ggacgattta ttaattactt
     4561 tagacagtat tcccgtttta caattaccag ccacaggtaa caaagcaaca ataaatagta
     4621 aaaaaaggga tattattctg cgttgtaaaa atttattagg tcaacaaaat caaacatcac
     4681 ggaaaatgca ggtgtattta tcaagttctg acttgttaac caacagcaac acaatactga
     4741 aaggtgcgga agataatgcg taggatttat tcttgaaagt aatggttcgc cagtcacact
     4801 tttaaatatc actaacagca gtaaaggata cacaaattta aaagaaattg cggcaaagtc
     4861 aaaacttaca gatacaacgg tttcaattcc gataacagcc agttactacg tctacgatac
     4921 aaacaaagtt aaatctggcg cactggaggc aaccgcatta atcaacgtga aatacgacta
     4981 ataacaagca aaacgagcaa gtggctaata ataaaaaata acaaggtgtc tgcatttccc
     5041 cctcatgatg agggggcttt ttttagcgat gataaaaaat ctcaccgtcg taggctttaa
     5101 tgattttacc gtcggtgtcg ctgatcaaca cgtacgcgcc acccatatag gtccagtgcg
     5161 tcccggcatc aggcgcgggc aggttacgta actgccactg cttaatgttg tactcatcgg
     5221 tgcggtacat ctctgggacg gtatcacctg gtttgaaatg cttaaagtca gcaatgaaac
     5281 tacttaactc atacttagaa attcccgagg tattggtcga acccgcggtg gctggtgcgg
     5341 cccaggcggc actggtaacc agcaaaagca cacccagcaa catcttattt ttaatggtca
     5401 taaactctcc actttgggga tctgtaatat cagtcgcata tctttcctgt catacaatat
     5461 aactgacaag cgatattttg ttagtgatcc catgatttcg taaaaagtgt aaatgaaagg
     5521 atgccctaaa agcatcctta gcgagcagag agtcagcgga aatagctgat ttaagaatca
     5581 tcctgggctg aagataacat gcaagcctaa aggattgagt tatcgcatta tgcccatgaa
     5641 gccatgtacg cccatataca caccgactaa gccaatcaac agactggaaa aatagggggc
     5701 gcgtttagcg agagtgttaa atccgctcca gcgttttgcg acctgctgaa cgctgattgc
     5761 tgcgccaacg cctacggtga caagcgttaa cgccaggcca atgctgaaac tgacgaccag
     5821 tgttgcgccc agtgtcaggg ctttcaactg aatgcatatc aacagcacgg taattgctgc
     5881 cgggcagggg ataaagccac cggttaagcc aaataacaaa atttgccagt tgatgacctc
     5941 tctaccatca aagcgtcgtt taatgtcatt ggcatgggct cgtgcatggg catcctgata
     6001 ctcgccatgt tcgtgatggt gatgatgtcc ttggtcgtgg tgatgttcgt gatcgtgatg
     6061 atggtgatga tgctcataat catgcccgtg catattctcc agccagttgc gttcgccgcg
     6121 ctaggtacgc cagaacatcc agaacgcggt gccaataatg atcactgcgg aaatcagctg
     6181 gagccacggt tctgctgatt gagcagtaaa gcgcttactg atcaccatcc cgccaaaggc
     6241 aatcaaccag accaccgcgg tatgcgaaat ggttgctgcc agtccgagca tcaccgcctg
     6301 tttaatggtg cctttgatgg cgatgataaa cgccgccatc atcgtttttg agtgccctgg
     6361 ttccaggcca tgaagcgcac caagtaagat ggcgctgggg atgaagaacc aggcgtttcc
     6421 ttgctgaaga agagttgtaa attcggtcat gataataatt cttagtatta attcggcaat
     6481 ctgattctac tcccccccag tacctgatac taccccccca gtagattaat agtgctatga
     6541 tttttcatat tcttgttaac caggtgttgc catgtctcat acaatccgtg ataaacagaa
     6601 actgaaagcg cgtgccagta agattcaggg ccaggtcgtg gcgctcaaga aaatgctcga
     6661 cgagccgcac gaatgcgctg cagttttaca acagattgct gctatccgtg gagcggtaaa
     6721 cggtctgatg cgggaagtga ttaaaggtca tctgacggaa cacatcgttc accaggggga
     6781 tgagctaaaa cgtgaagaag atctggatgt cgttctgaag gtgctggatt catatatcaa
     6841 ataatttatt aacgcgattg taaaactgcc gtttttcctc gtttacaacg ggggcgctgg
     6901 acattaccat cctcctctgc gatttatcat cgcaaccaaa cgactcgggg tgcccttctg
     6961 cgtgaaggct gagaaatacc cgtatcacct gatctggata atgccagcgt agggaagtca
     7021 cggaccacca ggtcattgct tcttcacgtt atggcaggag caaactatgc aagtcgacct
     7081 gctgagttca gcgcaatctg cgcacgcgtt acaccttttt caccaacatt cccctcttgt
     7141 gcactgcatg accaatgatg tggtgcaaac ctttaccgcc aataccttgc tggcgctcgg
     7201 tgcatcgcca gcgatggtta tcgaaaccga agaggccagt cagtttgcgg ctatcgccag
     7261 tgccttgttg attaacgttg gcacactgac gcagccacgc gcacaggcga tgcgtgctgc
     7321 cgttgagcaa gcaaaaagct ctcaaacacg ctggacgctt gatccagtag cggttggtgc
     7381 gctcgattat cgccgccatt tttgtcatga acttttatct tttaaaccgg cagcgatacg
     7441 tggtaatgct tcggaaatca tggcattagc tggcattgct aatggcggac ggggagtgga
     7501 taccactgac gccgcagcta acgcgatacc cgctgcacaa acactggcac gggaaactgg
     7561 cgcaatcgtc gtggtcactg gcgaggtgga ttatgttacc gatggacatc gtatcattgg
     7621 tattcacggt ggcgatccgt taatgaccaa agtggtagga actggctgtg cattatcggc
     7681 ggttgtcgct gcctgctgtg cgttaccagg cgatacgctg gaaaatgtcg catctgcctg
     7741 tcactggatg aaacaagccg gagaacgcgc agtcgccaga agcgaggggc caggcagttt
     7801 tgttccacat ttccttgatg cgctctggca attgacgcag gaggtgcagg catgaaacga
     7861 attaacgctc tgacgattgc cggtactgat ccgagtggtg gtgcggggat tcaggccgat
     7921 cttaaaacct tctcggcact tggcgcttat ggttgctcag ttattactgc actggtggcg
     7981 caaaataccc gtggcgtaca gtcggtgtat cgcattgagc ctgattttgt cgccgcccag
     8041 ctcgattcgg tgttcagcga tgtgcgaatc gataccacta aaatcggtat gttggcggaa
     8101 accgatattg ttgaagcggt ggcagaacgg ttgcaacgtt atcagatcca aaacgtggta
     8161 ctcgacaccg ttatgctggc aaaaagcggc gacccgctgc tttcaccttc ggcggttgct
     8221 acgctgcgca gtcgattatt gccacaggtt tcattaataa cgccaaactt gcccgaagct
     8281 gccgccttgc tcgacgcgcc acacgcgcgc accgaacagg aaatgctgga acaagggcga
     8341 tcgctgttgg cgatgggctg tggcgcagtg ctaatgaaag gtggtcatct ggatgatgag
     8401 caaagcccgg actggctgtt tacccgcgag ggtgaacaac ggtttaccgc accgcgcatt
     8461 atgaccaaaa acacccacgg cactggttgt acactctctg cggcgttggc tgcactacgc
     8521 ccgcgccata caaactgggc tgacaccgta caggaggcaa aaatctggct ttcatcggcg
     8581 ttagcccagg ccgacacgct ggaagttggt cacggtattg gtccggttca ccacttccac
     8641 gcctggtggt gagcaccgta ctatagctgc tttggttatt gtcagccttt ggcactgaca
     8701 ttcagggatg aaaccaatgc agctaagaat taccagtcga aaaaaactca ccgctctatt
     8761 atgcgctctt gggctaattt ccatcgttgc aatttatccg cgtcagacgg tgaatttttt
     8821 ctattcgaca gcagttcaga ttacagacta catccacttc tacggttatc gcccggttaa
     8881 atctttcgct attcgcattc ctgccagtta caccattcac ggattagatg tttcgcgctg
     8941 gcaggagcgt atcgactggc agcgggtggc aaaaatgcgc gacaacgata tccgcttaca
     9001 gtttgctttt attaaggcta cggaaggcga aaagctggtg gacccctatt tttcgcgtaa
     9061 ctggcaatta agccgcgaaa atggcctgct gcgcggggcg tatcattatt tctccccgtc
     9121 ggtatccgct tcagttcagg cgcgattatt tctgcaaacg gtggatttct cacaaggcga
     9181 tttccctgcc gtgctggacg tagaagaacg gggaaaatta tcggcaaaag aattacgcaa
     9241 gcgggtaagt cagtggctaa aaatggtcga aaaacgcacg ggaaaaaagc cgattattta
     9301 ctcaggagcc gttttttatc acaccaatct ggcgggctat ttcaatgaat atccgtggtg
     9361 ggtggctcac tattatcaac gtcgtccgga caatgacggc atggcctggc gcttctggca
     9421 gcattccgac cgtggacagg tagatggcat caatggtccg gtggatttta atgtgtttaa
     9481 tggcacgggg atgagttgca ggcattcgtt gatgggatta aagaaacgcc ttaaataaga
     9541 agcagtgata tactcccacc agactccctg gcaggaaaaa gacaaatgga acaagcgcat
     9601 acccagctga ttgcccaact gaacgagcgt attttagcgg cggataacac gccgctttat
     9661 attaagtttg ccgaaacggt aaaaaatgcc gtgcgcagcg gggtgctgga gcatggcaat
     9721 attttgcccg gtgagcgtga tttaagtcag ttaaccggcg tttcgcgcat tacggtgcgc
     9781 aaggcgatgc aggcgctgga agaagagggc gtggtgacgc gttcgcgcgg ttacggcacg
     9841 cagatcaaca acatcttcga atactcgttg aaagaagcga gaggtttttc tcagcaggtg
     9901 gtattgcgcg ggaaaaagcc cgatacgcta tgggttaata agcgtgtcgt gaaatgtccc
     9961 gaagaagttg cgcagcagct ggcggtcgaa gcgggaagtg atgtcttttt gcttaagcgt
    10021 attcgctatg tcgatgaaga agcggtatcg attgaggaat cgtgggttcc ggcgcattta
    10081 attcatgatg ttgatgccat cggaatttct ctttatgact atttccgcag ccagcacatt
    10141 tacccacaac gtacgcgttc ccgcgttagc gcccggatgc cggatgccga gtttcagtca
    10201 catattcagt tagatagcaa aataccggtg ctggtgatca agcaagtggc gcttgaccaa
    10261 cagcaacggc ctattgagta cagcattagc cactgtcgca gcgatctata cgtttttgtg
    10321 tgcgaggagt agttcctcgc gcgttggcgc acaatcaccg ccccgatgcc cgacaaccca
    10381 cgacgccact gcattgccca gtaatacggc atccgccagt ggcagaccag aggccagccc
    10441 ggcaagtacg ccacctgcat gactgtcgcc cgccccaatg gtgtctacaa cttgcgttgg
    10501 aaatgccgga atgcagccag aagcgtcgtt gctgaaatac catgcgcctt ctttatcgag
    10561 acgaacgatc aacggcgcgg caaatttctc ctgccattgc ttgccaagtg ttgttatctc
    10621 tgcggataaa gcaaaacgtt cggcggcaat ctcagcctct tgacgattga gtgacactaa
    10681 aggtcgacag gccatgatcc gcgccagtaa tgcgtccggg atatcgccaa tacgtgggca
    10741 aaaatcgata aacggcgtca cgtcttgcag cttttccagc cattccacta acaattcgcc
    10801 gcagggcgag gccagttgat aaccggaaaa atagagcagg ttgccaggcg caacggttaa
    10861 tcgtgccaac cattggcgat tccattgatt ttccacaccg ctgaatgaca taaaagtgcg
    10921 ttcgccatcc ggctcaacca gtgccagaca ccagccgtta tcgctttcgg cgttatcgat
    10981 caggctgatt aacccctctt tcgccatccg gttgcgaata atctccgccc acacgccctg
    11041 accgagcggc aaggcattac ccgcttcgat gccgaggcgc tttaacgcca cggcaatatt
    11101 cagtgcgcag ccgccaacgt taacgctctg ctgtttcagt tcgatatcgc acccacgcca
    11161 ggggagggca taagcgtcgg cgatcacgtc aatgaccgcc gcgccgacga ccatcaccgg
    11221 ctgacgcgtg gttaattcag gcagcagtgt gtgtaatcga gcgccgctca taccgcctcc
    11281 cgttgttgac gatatttcgc cagcgctgtg gcatagcggt tgaaatcaag ctgatttacc
    11341 gcatccagct ccgcctttaa cgcaggatcg acagcgttaa tgccatgcaa cgcgccgcaa
    11401 atcgccgtcg ccatagcacc tatggtgtct gtgtcgccgc caagattggc gcacaggacg
    11461 gcgcagcggt ttgggtcggt ttgtgccagt tcaactagcg caatggcgca cggaacggac
    11521 tcaatagtgc tggtgcctgc gccaacgacc tggtaaagct gttcgctggc ggattcggtg
    11581 ccgtcggcat tacgcacaat tttcagcgca atctccagac gtgccgccag tgaggcgctg
    11641 aacgtggtgg tgcgtttttg ctgtgcatgt cgcgcaattg aaggcaatga atcaacaatc
    11701 gctgaccagc gttctccgtc aatggcacga gaaatcgccc atgcgatgac taccgcgcct
    11761 gcaaccgcca gatcggattt atgggtcggg ctggacgcca gcgccacatc atcaataaag
    11821 gaatcaacat cacgcgctgg aagcaaacaa cctaatggcg agacgcgcat cgctgcgccg
    11881 ttggttacgc cgttgttttc cagttcagca acgggtttac cgtcgcgaat ggcgttaagc
    11941 gcaatcttcg aggtcggacc tagtacgttt ttgttaaagg cgtcgaaacg caacgcccag
    12001 tcgagaatat tacgcccaat cagattcgga tcgatcttgc cttcacattc cagtaacgca
    12061 tccgccagac acaggccatc gaggtatcgt cggtgaattc ggcgcggtta aaataacagg
    12121 ccgcgttatt ctcctttggt ccaggcagaa aacggtcaat ccagccaaag tgtgctttaa
    12181 cgcggctgcg tggccaaagc tcggagggca tccccatcgc atcccctaac gcctgcccat
    12241 aaagagcacc gagaatacgt tctgttttca tttaacttcc ccttgaatcg gaatttacgg
    12301 gagcatagta atgacagata ttgcgcagtt gcttggcaaa gacgccgaca accttttaca
    12361 gcaccgttgt atgactattc cttctgacca gctttatctc cccggacatg actacgtaga
    12421 ccgcgtgatg attgacaata atcgcccgcc agcggtgtta cgtaatatgc agacgttgta
    12481 caacaccggg cgtctggctg gcacaggata tctttctatt ctgccggttg accagggcgt
    12541 tgagcactct gccggagctt catttgctgc taacccgctc tactttgacc cgaaaaacat
    12601 tgttgaactg gcgatcgaag cgggctgtaa ctgtgtggca tcaacttacg gcgtgttggt
    12661 gtcggtatcg cggcgctatg cgcatcgcat tccatttctc gtcaaactta atcacaacga
    12721 gacgctaagt tacccgaata cctacgatca aacgctgtat gccagcgtgg agcaggcctt
    12781 caacatgggc gcggtggcgg ttggtgcgac tatctatttt ggctcggaag agtcacgtcg
    12841 ccagattgaa gaaatttctg cggcctttga acgcgcgcac gagctgggca tggtgacagt
    12901 gctgtgggcc tatttgcgaa actccgcctt taagaaagat ggcgttgatt accatgtttc
    12961 cgccgacctg actggtcagg cgaaccatct ggcggcaacc ataggtgcag atatcgtcaa
    13021 acaaaaaatg gcggaaaata acggcggcta taaagcgatt aattacggtt ataccgacga
    13081 tcgcgtgtac agcaagttaa ccagcgaaaa cccgattgat ctggtgcgtt atcagttagc
    13141 taactgctat atgggccggg ctgggttgat aaactccggc ggtgctgcag gcggtgaaac
    13201 tgacctcagc gatgcagtgc gtactgcggt tatcaacaaa cgcgcaggcg gaatggggct
    13261 gattcttgga cgtaaggcgt tcaagaaatc gatggctgac ggcgtgaaac tgattaatgc
    13321 cgtgcaggac gtttatctcg atagcaaaat tactattgcc tgatacgcct catacgaaca
    13381 gcaccacccc gtaggacgga taagacgttt acactgcatc cggcaaccta tgcctgatgc
    13441 gacgcggaag cgtcttatca tgcctacata gcactgccac gtatgtttac accgcatccg
    13501 gcataaaaac acgcgcactt tgctacggct tccctatcgg gaggccgttt ttttgccttt
    13561 cgctcctcga acaattttca tattgttgtt tttgcgatca ttatctcgac atttaaaagc
    13621 aaataatttc attatatttt gaaatcgaaa gcaaacgaca agatatgaaa atgtacgtgg
    13681 tatcgacaaa gcagatgctg aacaacgcac agcgcggcgg ttatgcggtt ccggcattca
    13741 atattcacaa tctcgaaacg atgcaagtgg tggtggaaac cgctgccaac ctgcatgcgc
    13801 cggtcatcat cgccggaacg cctggcacat ttactcatgc tggtacagaa aatctgttgg
    13861 cgctggtcag cgcgatggcg aagcaatatc accatccact ggcaattcat ctcgaccatc
    13921 acacgaaatt tgacgatatc gctcagaagg ttcgttctgg cgtgcgctca gtcatgattg
    13981 acgcctcgca tttgcctttt gcgcaaaata tttcacgggt caaagaggtg gtggattttt
    14041 gccatcgctt tgatgtcagc gtcgaggcgg agctggggca acttggcggc caggaagatg
    14101 atgtgcaagt caatgaagtc gatgcgttgt acaccaaccc cgcacaggcg cgtgaatttg
    14161 ccgaggcaac cgggattgat tccctggcgg tcgccatcgg cacggctcac ggtatgtatg
    14221 ccagcgcacc ggtgcttgat ttttctcgac tggagaacat tcgccagtgg gtgaacttac
    14281 cgctggtgct gcatggcgcg tcaggattat cgactaagga tattcagcaa accatcaaac
    14341 tggggatatg caaaatcaac gttgcaacgg agctgaaaaa tgccttcttg cagtcgttaa
    14401 aaaattacct gaccgagcac cccgaagcga ccgatccccg ggattatttg cagtcggcta
    14461 aatccgcaat gcgcgatgtg gtgagcaaag tgattgccga ttgtggctgc gagggcaggg
    14521 cataacgcac ctgccattta acaaggaaaa aacatgaaaa cgttaattgc ccggcataaa
    14581 gctggtgaac atatcggcat atgttcagtc tgttctgccc atccgttggt tatcgaagcg
    14641 gcgctggcat ttgatcgcaa cagcacgcgc aaagtgctga ttgaagcaac gtcaaaccag
    14701 gtcaatcaat ttggcggtta taccggaatg acaccggcag actttcgcga atttgttttt
    14761 acgattgccg ataaagttgg gtttgcacgc gaacgcatta ttctcggcgg cgatcatctg
    14821 ggaccaaact gctggcagca agaaaatgcg gatgcggcga tggaaaaatc cgtcgagctg
    14881 gtaaaagcat atgttcgtgc tggcttcagt aaaattcatc ttgatgcgtc aatgtcctgc
    14941 gcgggggata ccataccgtt agcaccagaa acggttgcgg aacgagctgc tgtgctttgc
    15001 tttgctgcgg aaagtgtggc gacagattgc cagcgtgagc aactgagcta tgtcattggc
    15061 actgaagttc cggttccggg cggtgaggcc agcgccattc agtcagtaca catcacccat
    15121 gttgaagatg ccgccaatac tttacgtacg catcaaaagg cctttattgc ccgtgggctg
    15181 acagaggcgt taacacgtgt gattgccatc gtggtgcagc cgggtgtgga atttgatcac
    15241 agcaatatta tccattatca gccgcaggaa gcgcaggcgc tggcgcaatg gatagaaaac
    15301 acccgaatgg tttatgaagc acattctacc gattaccaga cccggacggc ttattgggaa
    15361 ttagtccgcg atcactttgc aatattgaaa gtcggtcacg cattaacctt tgctttacgc
    15421 gaggcgatat ttgcactggc acaaattgag caggaactta tcgcccccga aaatcgcagc
    15481 ggttgcctgg cggtaattga agaagtgatg ctcgacgaac cgcaatactg gaaaaaatat
    15541 tatcgcacgg gttttaacga ttcattactg gatattcgtt acagcctgtc ggatcgtatt
    15601 cgttattact ggccgcatag tcggattaaa aatagcgttg aaacgatgat ggtgaatctg
    15661 caaggcgtgg acatcccact gggcatgatt agtcagtatc ttcccaaaca atttgaacgc
    15721 attcagtccg gggaattatc agcaatgccg catcagctta ttatgaataa aatttatgat
    15781 gttttgcgcg cctatcgcta cggctgtgcg gaataaggac ggtatatgac taacctgttt
    15841 gttcgtagcg gaatttcttt tgtcgatcgt agcgaagttt taacctatat cggtaatgag
    15901 atgctcgcga aaggtgtggt tcatgatacc tggccacagg cattaattgc cagagaagca
    15961 gaattcccta ccgggataat gcttgagcag cacgccattg caataccgca ttgtgaggcg
    16021 attcatgcta agtcgtcagc catttatctg ttaaggccaa caaataaagt tcattttcag
    16081 caagcggatg atgataacga cgtggcggta tcgttggtta ttgcgttgat tgtggaaaat
    16141 ccgcagcagc aattgaaact tttacgctgt ttatttggca agttacaaca gcccgatatc
    16201 gtcgagacac taattactct tcctgaaact cagttaaagg aatacttcac aaagtatgtt
    16261 ttagattcag acgaataaat ccctctgtaa caataaaaag gactatttat gaaacgcaag
    16321 attattgtcg cttgcggagg cgcggttgcg acctctacga tggcggcgga agaaattaaa
    16381 gagttgtgtc agagtcataa tattcctgtt gaattaatcc agtgtcgggt taatgaaata
    16441 gaaacctata tggatggtgt gcatttgata tgtaccactg ccagggtgga tcgtagtttt
    16501 ggcgatattc cgttagttca cggcatgcct tttgtttctg gtgtcggtat cgaagcatta
    16561 caaaataaaa ttctgactat cttacaggga tgacctatgt tttcagaagt catgcgttat
    16621 attctcgacc tcggccctac agtgatgctg ccggttgtca tcattatttt ttctaaaata
    16681 ttaggcatga aggcaggcga ttgctttaaa gcgggtctgc atatcgggat tggctttgtt
    16741 ggcattggcc ttgtgattgg cttaatgctg gattccattg gtccggcggc gaaagcgatg
    16801 gcggaaaatt tcgacctgaa tctgcatgtg gtcgatgtcg gctggccggg ctcttcacca
    16861 atgacctggg cgtcgcaaat tgcgctggtg gcgattccga ttgcgatatt ggttaacgtg
    16921 gcgatgttac tgacccgtat gacgcgggtg gtaaatgttg atatctggaa tatctggcat
    16981 atgaccttca ccggcgcgtt gctgcacctg gcaaccggtt catggatgat agggatggcg
    17041 ggtgtggtaa ttcacgcggc gtttgtttat aagctcggcg actggtttgc ccgcgatacc
    17101 cgaaatttct ttgagctgga aggtattgct attccgcacg gtacgtcggc gtatatgggg
    17161 ccgattgcgg tgctggtcga tgctatcatc gagaaaatcc caggcgttaa ccgaattaaa
    17221 tttagcgccg acgatattca gcgcaaattt ggcccttttg gcgaacctgt caccgtgggt
    17281 tttgtgatgg ggctgattat cggcatcctc gcgggttacg atgtcaaagg tgtattgcag
    17341 ctggcggtaa aaacggcggc agttatgctg ctgatgccac ggatgattaa acccatcatg
    17401 gatggtttaa cgcccatcgc taagcaggcc cgtagtcgtt tacaggcgaa gttcggcggt
    17461 caggagttcc tgattggcct ggatcccgca ttactgctgg ggcatacggc ggtggtatcg
    17521 gcaagcctga tttttattcc gctcaccatt ttaattgctg tttgtgttcc gggtaatcag
    17581 gtactgccgt ttggcgatct tgccactatc ggcttcttcg tggcgatggc ggtcgcggtg
    17641 catcgtggaa atctgttccg caccttaatc tcgggtgtca tcattatgag catcaccctg
    17701 tggatcgcga cgcaaactat tggtttgcac acccaaccgg cggctaatgc tggggcgtta
    17761 aaagccgggg gtatggtggc ttcaatggat cagggcggtt ctcccattac ctggttactg
    17821 attcaggttt tctccccgca aaatattccc ggtttcatta ttatcggcgc aatttatctg
    17881 accggtattt tcatgacctg gcgtagagcg cgtggtttta ctaaacaaga gaaagccgtt
    17941 ctcgcagaat aatttttacc tgagggggag gttatccccc ttaacaa
//
LOCUS       DAEBXN010000086        17786 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.086, whole genome shotgun sequence.
ACCESSION   DAEBXN010000086 DAEBXN010000000
VERSION     DAEBXN010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17786
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.086"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(84..1367)
                     /gene="gltA"
                     /locus_tag="KJL99_002956"
     CDS             complement(84..1367)
                     /gene="gltA"
                     /locus_tag="KJL99_002956"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HBD4437610.1"
                     /translation="MADTKAKLNLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSYGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            complement(1573..1680)
                     /locus_tag="KJL99_002957"
     CDS             complement(1573..1680)
                     /locus_tag="KJL99_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4437611.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            2061..2465
                     /gene="sdhC"
                     /locus_tag="KJL99_002958"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             2061..2465
                     /gene="sdhC"
                     /locus_tag="KJL99_002958"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HBD4437612.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILQWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            2459..2806
                     /gene="sdhD"
                     /locus_tag="KJL99_002959"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             2459..2806
                     /gene="sdhD"
                     /locus_tag="KJL99_002959"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HBD4437613.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            2806..4572
                     /gene="sdhA"
                     /locus_tag="KJL99_002960"
     CDS             2806..4572
                     /gene="sdhA"
                     /locus_tag="KJL99_002960"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HBD4437614.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALHDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            4588..5304
                     /gene="sdhB"
                     /locus_tag="KJL99_002961"
     CDS             4588..5304
                     /gene="sdhB"
                     /locus_tag="KJL99_002961"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HBD4437615.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            5605..8406
                     /gene="sucA"
                     /locus_tag="KJL99_002962"
     CDS             5605..8406
                     /gene="sucA"
                     /locus_tag="KJL99_002962"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HBD4437616.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELISAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPNSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            8421..9638
                     /gene="odhB"
                     /locus_tag="KJL99_002963"
     CDS             8421..9638
                     /gene="odhB"
                     /locus_tag="KJL99_002963"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HBD4437617.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEEPTRLLLDV"
     gene            9732..10898
                     /gene="sucC"
                     /locus_tag="KJL99_002964"
     CDS             9732..10898
                     /gene="sucC"
                     /locus_tag="KJL99_002964"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HBD4437618.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            10898..11767
                     /gene="sucD"
                     /locus_tag="KJL99_002965"
     CDS             10898..11767
                     /gene="sucD"
                     /locus_tag="KJL99_002965"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HBD4437619.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGASASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(11870..12592)
                     /gene="mngR"
                     /locus_tag="KJL99_002966"
     CDS             complement(11870..12592)
                     /gene="mngR"
                     /locus_tag="KJL99_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HBD4437620.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITVQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            12762..14678
                     /gene="mngA"
                     /locus_tag="KJL99_002967"
     CDS             12762..14678
                     /gene="mngA"
                     /locus_tag="KJL99_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HBD4437621.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LKEVYRRESLGPTALSEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPLNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQSLTLERGDETRTLQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLIRWMKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            14696..17329
                     /gene="mngB"
                     /locus_tag="KJL99_002968"
     CDS             14696..17329
                     /gene="mngB"
                     /locus_tag="KJL99_002968"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HBD4437622.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRLKKLVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITCTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVMPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMEKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKVAHARIENKIVNLLEPLATLARTLGFGYHHGLLEKMWKEILK
                     NHAHDSIGCCCRDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITSANAKPQCEIIHEAWQSRAVIR
                     YEMAVPRNLQQRSARQSTGRVDVELVVTLSHNNRRIDVDINLDNQADDHRLRVLIPTS
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQESWKEAPVPVWNMLNYAALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPRRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
BASE COUNT         4337 a         4422 c         4804 g         4223 t
ORIGIN      
        1 acaatttttt gtgtttaggc atataaaaat taacccgcca tttgaacggc gggttaaaat
       61 atttacaact tagcaatcaa ccattaacgc ttgatatcgc ttttaaagtc gcgtttttca
      121 tatcctgtat acagctgacg cggacgggca atcttcatac cgtaactgtg catttcgctc
      181 cagtgggcga tccagccaac ggtacgtgcc attgcgaaaa tgacggtgaa catggaagac
      241 ggaataccca tcgctttcag gatgatacca gagtagaaat cgacgttcgg gtacagtttc
      301 ttctcgataa agtacgggtc gttcagcgcg atgttttcca gctccatagc cacttccagc
      361 aggtcatctt tggtgcccag ctctttcaga acttcatggc aggtttcacg cattacggtg
      421 gcgcgcgggt cgtaattttt gtacacgcgg tgaccgaagc ccatcaggcg gaaagaatca
      481 tttttatctt tcgcacgacg aacaaattcc ggaatgtgtt taacggagct aatttcttcc
      541 agcattttca gcgccgcttc gttagcacca ccgtgcgcag gtccccacag tgaagcaata
      601 cctgctgcga tacaggcaaa cgggttcgcc cccgaagagc cagcggtacg cacggtggag
      661 gtagaggcgt tctgttcatg gtcagcgtgc aggatcagaa tacggtccat agcacgttcc
      721 agaatcggat taacttcata cggttcgcac ggcgtggaga acatcatatt caggaagtta
      781 ccggcatagg agagatcgtt gcgcggataa acaaatggct gaccaatgga atacttgtaa
      841 cacatcgcgg ccatggtcgg cattttcgac agcaggcgga acgcggcaat ttcacgatga
      901 cgaggattgt taacatccag cgagtcgtga tagaacgccg ccagcgcgcc ggtaatacca
      961 cacatgactg ccattggatg tgagtcgcga cggaaagcgt ggaacagacg ggtaatctgc
     1021 tcgtggatca tggtatgacg ggtcaccgta gttttaaatt cgtcatactg ttcctgagtc
     1081 ggtttttcac cattcagcag gatgtaacaa acttctaggt agttagaatc ggtcgccagc
     1141 tgatcgatcg ggaaaccgcg gtgcagcaaa ataccttcat caccatcaat aaaagtaatt
     1201 ttagattcgc aggatgcggt tgaagtgaag cctgggtcaa aggtgaacac accttttgaa
     1261 ccgagagtac ggatatcaat aacatcttga cccagcgtgc ctttcagcac atccagttca
     1321 acagctgtgt ccccgttgag gttgagtttt gcttttgtat cagccattta aggtctcctt
     1381 agcgccttat tgcgtaagac tgccggaact taaatttgcc ttcgcacatc aacctggctt
     1441 tacccgtttg ttatttggct cgccgctctg tgaaagaggg gaaaacctgg gtacagagct
     1501 ctgggcgctt gcaggtaaag aatccattga tgacgaataa atggcgaatc aagtacttag
     1561 caatccgaat tattaaactt gtctaccact aataactgtc ccgaatgaat tggtcaatac
     1621 ttccacactg ttacataagt taatcttagg tgaaataccg acttcataac ttttacgcat
     1681 tatatgcttt tcctggtaat gtttgtaaca actttgttga atgattgtca aattagatga
     1741 ttaaaaatta aataaatgtt gttatcgtga cctggatcac tgttcaggat aaaacccgac
     1801 aaactatatg taggttaatt gtaatgattt tgtgaacagc ctatactgcc gccaggtctc
     1861 cggaacaccc tgcaatcccg agccacccag cgttgtaacg tgtcgttttc gcatctggaa
     1921 gcagtgtttt gcatgacgcg cagttatgga aaggacgctg tctgacccgc aagcagaccg
     1981 gaggaaggaa atcccgacgt ctccaggtaa cagaaagtta acctctgtgc ccgtagtccc
     2041 cagggaataa taagaacagc atgtgggcgt tattcatgat aagaaatgtg aaaaaacaaa
     2101 gacctgttaa tctggaccta cagaccatcc ggttccccat cacggcgata gcgtccattc
     2161 tccatcgcgt ttccggtgtg atcacctttg ttgcagtggg catcctgcag tggcttctgg
     2221 gtaccagcct ctcttcccct gaaggtttcg agcaagcttc cgcgattatg ggcagcttct
     2281 tcgtcaaatt tatcatgtgg ggcatcctta ccgctctggc gtatcacgtc gtcgtaggta
     2341 ttcgccacat gatgatggat tttggctatc tggaagaaac attcgaagcg ggtaaacgct
     2401 ccgccaaaat ctcctttgtt attactgtcg tgctttcact tctcgcagga gtcctcgtat
     2461 ggtaagcaac gcctccgcat taggacgcaa tggcgtacat gatttcatcc tcgttcgcgc
     2521 taccgctatc gtcctgacgc tctacatcat ttatatggtc ggttttttcg ccaccagtgg
     2581 cgaactgaca tatgaagtct ggatcggttt cttcgcctct gcgttcacca aagtgttcac
     2641 cctgctggcg ctgttttcta tcttgattca tgcctggatc ggcatgtggc aggtgttgac
     2701 cgactacgtt aaaccgctgg ccttgcgcct gatgctgcaa ctggtgattg tcgttgcact
     2761 ggtggtttac gtgatttatg gattcgttgt ggtgtggggt gtgtgatgaa attgccagtc
     2821 agagaatttg atgcagttgt gattggtgcc ggtggcgcag gtatgcgcgc ggcgctgcaa
     2881 atttcccaga gcggccagac ctgtgccctg ctctctaaag tcttcccgac ccgttcccat
     2941 accgtttctg cgcaaggcgg cattaccgtt gcgctgggta atacccatga agataactgg
     3001 gaatggcata tgtacgacac cgtaaaaggg tcggactata tcggtgacca ggacgcgatt
     3061 gaatatatgt gtaaaaccgg gccggaagcg attctggaac tcgaacacat gggcctgccg
     3121 ttctcgcgtc tcgatgatgg tcgtatctat caacgtccgt ttggcggtca gtcgaaaaac
     3181 ttcggcggcg agcaggcggc acgcactgcg gcggctgctg accgtaccgg tcacgcactg
     3241 ttgcacacgc tttatcagca gaacctgaaa aaccacacca ccattttctc cgagtggtat
     3301 gcgctggatc tggtgaaaaa ccaggatggc gcggtggtgg gttgtaccgc actgtgcatc
     3361 gaaaccggtg aagtggttta tttcaaagcc cgcgctaccg tgctggcgac tggcggagca
     3421 gggcgtattt atcagtccac cactaacgcc cacattaaca ctggcgacgg tgtcggcatg
     3481 gctatccgcg caggcgtacc ggtgcaggat atggaaatgt ggcaattcca cccgaccggc
     3541 attgccggtg cgggcgtact ggtcaccgaa ggttgccgtg gtgaaggcgg ttatctgctg
     3601 aacaaacatg gcgaacgctt tatggagcgt tatgcgccga acgccaaaga cctggcgggc
     3661 cgtgatgtgg tggcgcgttc catcatgatt gaaatccgtg aaggtcgcgg ctgtgatggt
     3721 ccgtgggggc cacacgcgaa actgaaactc gatcacctgg gtaaagaagt tctcgaatcc
     3781 cgtctgccgg gtatcctgga gctttcccgt accttcgctc acgtcgatcc ggtgaaagag
     3841 ccgattccgg ttatcccaac ctgtcactac atgatgggcg gtattccgac caaagttacc
     3901 ggtcaggcgc tgactgtgaa tgagaaaggc gaagatgtgg ttgttccggg actgtttgcc
     3961 gttggtgaaa tcgcttgtgt atcggtacac ggcgctaacc gtctgggcgg caactcgctg
     4021 ctggacctgg tggtctttgg tcgcgcggca ggtctgcatc tgcaagagtc tatcgccgag
     4081 cagggcgcac tgcacgatgc cagcgagtct gatgttgaag cgtctctgga tcgcctgaac
     4141 cggtggaaca ataatcgtaa cggtgaagat ccggtggcga tccgtaaagc gctgcaagaa
     4201 tgtatgcagc ataacttctc ggtcttccgt gaaggtgatg cgatggcgaa agggcttgag
     4261 cagttgaaag tgatccgcga gcgtctgaaa aatgcccgtc tggatgacac ttccagcgag
     4321 ttcaacaccc agcgcgttga gtgcctggaa ctggataacc tgatggaaac ggcgtatgca
     4381 acggctgttt ctgccaactt ccgtaccgaa agccgtggcg cacatagccg cttcgacttc
     4441 ccggatcgtg atgatgaaaa ctggctgtgc cactccctgt atctgcctga gtcggaatcc
     4501 atgacgcgcc gaagcgtcaa catggaaccg aaactgcgcc cggcattccc gccgaagatt
     4561 cgtacttact aatgcggaga caggaaaatg agactcgagt tttcaattta tcgctataac
     4621 ccggatgttg atgatgctcc gcgtatgcag gattacaccc tggaagcgga agaaggtcgc
     4681 gacatgatgc tgctggatgc gcttatccag ctgaaagaga aagatcccag cctgtcgttc
     4741 cgccgctcct gccgtgaagg tgtgtgcggt tccgacggtc tgaacatgaa cggcaagaat
     4801 ggtctggcct gtattacccc gatttcggca ctcaaccagc cgggcaagaa gattgtgatt
     4861 cgcccgctgc caggtttacc ggtgatccgc gatttggtgg tagacatggg acaattctat
     4921 gcgcaatatg agaaaattaa gccttacctg ttgaataatg gacaaaatcc gccagctcgc
     4981 gagcatttac agatgccaga gcagcgcgaa aaactcgacg ggctgtatga atgtattctc
     5041 tgcgcatgtt gttcaacctc ttgtccgtct ttctggtgga atcccgataa gtttatcggc
     5101 ccggcaggct tgttagcggc atatcgtttc ctgatcgata gccgtgatac cgagactgac
     5161 agccgcctcg acggtttgag cgatgcattc agcgtattcc gctgtcacag catcatgaac
     5221 tgcgtcagtg tatgtccgaa ggggctgaac ccgacgcgcg ccatcggcca tatcaagtcg
     5281 atgttgttgc aacgtaatgc gtaaactgta ggcctgataa gacgcgcaag cgttgcatca
     5341 ggcaaccagt gccggatgcg gcgtgaacgc cttatccggc ctacaagtca ttacccgcag
     5401 gcctgataag cgcagcgcat caggcgtaac aaagaaatgc aggaaatctt taaaaactgc
     5461 ccctgacact aagacagttt ttaaaggttc cttcgcgagc cactacgtag acaagagctc
     5521 gcaagtgaac cccggcacgc acatcactgt gcgtggtagt atccacggcg aagtaagcat
     5581 aaaaaagatg cttaagggat cacgatgcag aacagcgctt tgaaagcctg gttggactct
     5641 tcttacctct ctggcgcaaa ccagagctgg atagaacagc tctatgaaga cttcttaacc
     5701 gatcctgact cggttgacgc taactggcgt tcgacgttcc agcagttacc tggtacggga
     5761 gtcaaaccgg atcaattcca ctctcaaacg cgtgaatatt tccgccgcct ggcgaaagac
     5821 gcttcacgtt actcttcaac gatctccgac cctgacacca atgtgaagca ggttaaagtc
     5881 ctgcagctca ttaacgcata ccgcttccgt ggtcaccagc atgcgaatct agatccgctg
     5941 ggactgtggc agcaagataa agtggccgat ctggatccgt ctttccacga tctgaccgaa
     6001 gcagacttcc aggaaacctt caacgtcggt tcatttgcca gcggcaaaga aaccatgaaa
     6061 ctcggcgagc ttatttccgc cctcaagcaa acctactgcg gcccgattgg tgccgagtat
     6121 atgcacatca ccagcaccga agaaaaacgc tggatccaac agcgtatcga gtctggtcgc
     6181 gcgactttca atagcgaaga gaaaaaacgc ttcttaagcg aactgaccgc cgctgaaggc
     6241 cttgagcgtt acctcggcgc aaaattccct ggcgcaaaac gcttctcgct ggaaggcggt
     6301 gacgcgttaa tcccgatgct taaagagatg atccgccacg ctggcaacag cggcacccgc
     6361 gaagtggttc tcgggatggc gcaccgtggt cgtctgaacg tgctggtgaa cgtgctgggt
     6421 aaaaaaccgc aagacttgtt cgacgagttc gccggtaaac ataaagaaca cctcggcaca
     6481 ggcgacgtga aataccacat gggcttctcg tctgacttcc agaccgatgg cggcctggtg
     6541 cacctggcgc tggcgtttaa cccgtctcac cttgagattg taagcccggt cgttatcggc
     6601 tctgttcgtg cccgtctgga cagacttgat gagccgaaca gcaacaaagt gctgccaatc
     6661 accatccacg gtgacgccgc agtgaccggg cagggcgtgg ttcaggaaac tctgaacatg
     6721 tcgaaagcgc gtggttatga agttggcggt acggtacgta tcgttatcaa caaccaggtt
     6781 ggtttcacca cctctaaccc gctggatgcc cgttctacac cgtactgtac tgatatcggt
     6841 aagatggttc aggcaccgat tttccacgtt aacgcggatg atccggaagc cgttgccttt
     6901 gtgacccgtc tggcgctcga tttccgtaac acctttaaac gtgatgtctt catcgacctg
     6961 gtatgctacc gccgtcacgg ccacaacgaa gccgacgagc cgagcgcaac ccagccactg
     7021 atgtatcaga aaatcaaaaa acatccgaca ccgcgcaaaa tctacgctga caagctggag
     7081 caggaaaaag tggcgacgct ggaagatgcc accgagatgg ttaacctgta ccgcgatgcg
     7141 ctggatgctg gcgattgcgt agtggcagag tggcgtccga tgaacatgca ctctttcacc
     7201 tggtcgccgt acctcaacca cgaatgggac gaagagtacc cgaacaaagt tgagatgaag
     7261 cgcctgcagg aactggctaa acgtatcagt acggtaccag aagcagttga aatgcagtct
     7321 cgcgttgcca agatttatgg cgatcgccag gcgatggcag ccggtgagaa actgttcgac
     7381 tggggcggtg cggaaaacct cgcttacgcc acgctggttg atgaaggcat tccggttcgc
     7441 ctgtcgggtg aagactccgg tcgcggtacc ttcttccacc gccacgcggt gatccacaac
     7501 cagtctaacg gttccactta cacgccgctg caacacatcc ataacggtca gggcgcgttc
     7561 cgtgtctggg actccgtact gtcagaagaa gccgtgctgg cgtttgaata tggttatgcc
     7621 accgcagaac cacgcaccct gaccatctgg gaagcgcagt tcggtgactt cgccaacggt
     7681 gcgcaggtgg ttatcgacca gttcatctcc tctggcgagc agaaatgggg ccggatgtgt
     7741 ggtctggtga tgttgctgcc gcacggttac gaagggcagg ggccggagca ctcctccgcg
     7801 cgtctggaac gttatctgca actttgtgct gagcaaaaca tgcaggtttg cgtaccgtct
     7861 accccggcac aggtttacca catgctgcgt cgtcaggcgc tgcgcgggat gcgtcgtccg
     7921 ctggtcgtga tgtcgccgaa atccctgctg cgtcatccgc tggcggtttc cagcctcgaa
     7981 gaactggcga acggcacctt cctgccagcc atcggtgaaa tcgacgagct tgatccgaag
     8041 ggcgtgaagc gcgtagtgat gtgttctggt aaggtttatt acgacctgct ggaacagcgt
     8101 cgtaagaaca atcaacacga tgtcgccatt gtgcgtatcg agcaactcta cccgttcccg
     8161 cataaagcga tgcaggaagt gttgcagcag tttgctcacg tcaaggattt tgtctggtgc
     8221 caggaagagc cgctcaacca gggcgcatgg tactgcagcc agcatcattt ccgtgaagtg
     8281 attccgtttg gggcttctct gcgttatgca ggccgcccgg cctccgcctc tccggcggta
     8341 gggtatatgt ccgttcacca gaaacagcaa caagatctgg ttaatgacgc gctgaacgtc
     8401 gaataaataa aggatacaca atgagtagcg tagatattct ggtccctgac ctgcctgaat
     8461 ccgtagccga tgccaccgtc gcaacctggc ataaaaaacc cggcgacgca gtcgtacgtg
     8521 atgaagtgct ggtagaaatc gaaactgaca aagtggtact ggaagtaccg gcatcagcag
     8581 acggcattct ggatgcggtt ctggaagatg aaggtacaac ggtaacgtct cgtcagatcc
     8641 ttggtcgcct gcgtgaaggc aacagcgccg gtaaagaaac cagcgccaaa tctgaagaga
     8701 aagcgtccac tccggcgcaa cgccagcagg cgtctctgga agagcaaaac aacgatgcgt
     8761 taagcccggc gatccgtcgc ctgctggctg aacacaatct cgacgccagc gccattaaag
     8821 gcaccggtgt gggtggtcgt ctgactcgtg aagatgtgga aaaacatctg gcgaaagccc
     8881 cggcgaaaga gtctgctccg gcagcggctg ctccggcggc gcaaccggct ctggctgcac
     8941 gtagtgaaaa acgtgtcccg atgactcgcc tgcgtaagcg tgtggcagag cgtctgctgg
     9001 aagcgaaaaa ctccaccgcc atgctgacca cgttcaacga agtcaacatg aagccgatta
     9061 tggatctgcg taagcagtac ggtgaagcgt ttgaaaaacg ccacggcatc cgtctgggct
     9121 ttatgtcctt ctacgtgaaa gcggtggttg aagccctgaa acgttacccg gaagtgaacg
     9181 cgtctatcga cggcgatgac gtggtttacc acaactattt cgacgtcagc atggcggttt
     9241 ctacgccgcg cggcctggtg acgccggttc tgcgtgatgt cgataccctc ggcatggcag
     9301 acatcgagaa gaaaatcaaa gagctggcag tcaaaggccg tgatggcaag ctgacggttg
     9361 aagatctgac cggtggtaac ttcaccatca ccaacggtgg tgtgttcggt tccctgatgt
     9421 ctacgccgat catcaacccg ccgcagagcg caattctggg tatgcacgct atcaaagatc
     9481 gtccgatggc ggtgaatggt caggttgaga tcctgccgat gatgtacctg gcgctgtcct
     9541 acgatcaccg tctgatcgat ggtcgcgaat ccgtgggctt cctggtaacg atcaaagagt
     9601 tactggaaga gccgacgcgt ctgctgctgg acgtgtagta gtttaagctt cacctgcact
     9661 gtagaccgac gtggataggg tgtcggtcta cggtttaaaa gataacgatt actgaaggat
     9721 ggacagaaca catgaactta catgaatatc aggcaaaaca actgtttgcc cgctatggct
     9781 taccagcacc ggtgggttat gcctgtacta ctccgcgcga agcagaagaa gccgcttcaa
     9841 aaatcggtgc cggtccgtgg gtagtgaaat gtcaggttca cgctggtggc cgcggtaaag
     9901 cgggcggtgt gaaagttgta aacagcaaag aagacatccg tgcttttgca gaaaactggc
     9961 tgggcaagcg tctggtaacg tatcaaacag atgccaatgg ccaaccggtt aaccagattc
    10021 tggttgaagc ggcgaccgat atcgctaaag agctgtatct cggtgccgtt gtagaccgta
    10081 gttcccgtcg cgtggtcttt atggcctcca ccgaaggcgg cgtggaaatc gaaaaagtgg
    10141 cggaagaaac tccgcacctg atccataaag ttgcgcttga tccgctgact ggcccgatgc
    10201 cgtatcaggg acgcgagctg gcgttcaaac tgggtctgga aggtaaactg gttcagcagt
    10261 tcaccaaaat cttcatgggc ctggcgacca ttttcctgga gcgcgacctg gcgctgatcg
    10321 aaatcaaccc gctggtcatc accaaacagg gcgatctgat ttgcctcgac ggcaaactgg
    10381 gcgctgatgg caacgccctg ttccgccagc ctgatctgcg cgaaatgcgt gaccagtcgc
    10441 aggaagatcc gcgtgaagca caggctgcac agtgggaact gaactacgtt gcgctggacg
    10501 gtaacatcgg ttgtatggtt aacggcgcag gtctggcgat gggtacgatg gacatagtta
    10561 aactgcacgg cggcgaaccg gctaacttcc tggacgttgg cggcggcgca accaaagaac
    10621 gtgtaaccga agcgttcaaa atcatcctct ctgacgacaa agtgaaagcc gttctggtta
    10681 acatcttcgg cggtatcgtt cgttgcgacc tgatcgctga cggtatcatc ggcgcggtag
    10741 cggaagtggg tgttaacgta ccggtcgtgg tacgtctgga aggtaacaac gccgaactcg
    10801 gcgcgaagaa actggctgac agcggcctga atattattgc agcaaaaggt ctgacggatg
    10861 cagctcagca ggttgttgcc gcagtggagg ggaaataatg tccattttaa ttgataaaaa
    10921 caccaaggtt atctgccagg gctttaccgg tagccagggg actttccact cagaacaggc
    10981 cattgcatac ggcactaaaa tggttggtgg cgtaactcca ggtaaaggcg gcactaccca
    11041 cctcggcctg ccggtgttca acaccgtgcg tgaagctgtt gctgctactg gcgcttccgc
    11101 gtctgttatt tacgtaccag caccgttctg caaagactcc attctggaag ctatcgatgc
    11161 aggcatcaaa ctgattatca ccatcactga aggcatcccg acgctggata tgctgaccgt
    11221 gaaagtgaaa ctggacgaag caggcgttcg tatgatcggc ccgaactgcc caggcgttat
    11281 caccccgggt gaatgcaaaa ttggtatcca gccgggtcac attcacaaac cgggtaaagt
    11341 gggtatcgtt tcccgttccg gtacactgac ctatgaagcg gttaaacaga ccacggatta
    11401 cggtttcggt cagtcgacct gtgtcggtat cggcggtgac ccgatccctg gctctaactt
    11461 tatcgacatt ctcgaaatgt tcgaaaaaga tccgcagacc gaagcgatcg tgatgatcgg
    11521 tgagatcggc ggtagcgctg aagaagaagc ggcagcgtac atcaaagagc atgttaccaa
    11581 gccagttgtg ggttacatcg ctggtgtgac tgcgccgaaa ggcaaacgta tgggccacgc
    11641 gggtgcgatc attgccggtg ggaaagggac tgcggatgag aaattcgctg ctctggaagc
    11701 cgcaggcgtg aaaaccgttc gcagcctggc ggatatcggt gaagcactga aaactgttct
    11761 gaaataaata tctgtaataa gaaatagccc tcgccgcttc cctctacagg aatggcgaag
    11821 ggctgtcggt ttcgacatgg ttggccatcg tatgatggcc tttttgtgct tatcgcgatg
    11881 attttcgctg cgctatcagg gtaaatttat agtcatcggt attaaaagcg ttgcggctat
    11941 attcaaacac ccgaccatca actaaatatc cacgcgatac tttttcaaga atcggctttg
    12001 tctggctgat attaagcaga cggctcatct cttcggttgg catcagagga atgatttcct
    12061 gttcgctacg atcgataacc atcttcttca cttcttcaat aaagtgatat ttcgaatttt
    12121 ccatgacctg ccaggtgaga tccgggaaca acgcaagcgg catccaggtt tcttccagcg
    12181 ccattggctt ttgcttgcga tagcgcacgc gcttcacatg ccaaacacga tcctgcacgg
    12241 tgatttgtag ctgttgctga agaaaatcgt cagccggaat cacttcaaat atcagaactt
    12301 cactgtgtgt atcgacgtga cggtccgaca gtttttcatc aaaactggtt aactgaaaaa
    12361 tatcgtaatt gacccgctct tctttgacgt aagtcccgct gccctgaatg ctttcgagga
    12421 tctgttgctc gactaactgg cgcaaagcct gacgcaccgt aacccggctg acgccaaact
    12481 ctgtttgtaa tgccgattcg gtaggtaacg catcgccagg tttaagctcg ccacgcgcaa
    12541 tttgctcacg aatgcgatca gcaatctgtc ggtataaggg tttgtgtccc attatttagc
    12601 atctcattaa tacgaattta gccattatgc ccgataaact catcctgtaa acaatacaaa
    12661 taaaatgcaa ataatttcaa tcaagtgaaa ttgattacgc aatggtattg ttttgtcggg
    12721 cacactggcg cgactataaa aacgatcaag tgaggatcat gatgaacctg acgactctga
    12781 cccaccgcga tgcgttgtgt ctgaatgcgc gctttaccag ccgtgaagag gccattcacg
    12841 cgttgactca acgtcttgct gctctgggga aaatttccag tactgagcaa tttctgaaag
    12901 aagtgtatcg ccgtgaaagc cttggcccga ccgccttaag tgaagggttg gctgtgccgc
    12961 atggcaaaac tgctgcggta aaagaagcgg cgtttgcggt cgccacactc agcgagccgc
    13021 ttcagtggga aggcgtggat ggcccggaag cggttgattt agtggtgttg ctggcgattc
    13081 ccctcaatga agcgggtact acgcacatgc aactgctgac agcgctgacc acgcgcctgg
    13141 cggatgatga gattcgggcg cgtatacagt cggcgacgac gcctgatgaa ttgctctcgg
    13201 cgctggatga caagggaggc acgcaacctt ctgcctcttt ttccaacgcg ccaactatcg
    13261 tttgcgtaac ggcctgcccg gcgggtattg ctcacaccta catggctgcg gagtatctgg
    13321 aaaaagccgg acgcaaactc ggcgtgaatg tctacgttga aaagcaaggt gctaacggta
    13381 ttgaagggcg tttaacggcg gatcaactca atagtgcaac tgcctgtatt tttgcggctg
    13441 aagtcgccat caaggagagt gagcgtttta acggcattcc tgcgctttct gtgcctgttg
    13501 ccgagccgat tcgccatgca gaagcgttga tccaacaatc gcttaccctc gagcgtggcg
    13561 atgagacgcg taccttacag caagatacgc aaccggtgaa aagcgtcaaa acggagctga
    13621 aacaggcact gttgagcggg atctcttttg ccgtaccgtt gattgtcgcg ggggggacgg
    13681 tgctggcggt cgcggtatta ctgtcgcaaa tcttcgggct acaagatctg tttaatgaag
    13741 aaaactcctg gctgtggatg taccgcaaac tgggcggcgg gctgctcgga attttgatgg
    13801 taccggtgct ggcagcctat accgcctatt cgctggcaga taaaccggcg ttagcgccag
    13861 gctttgcggc tggacttgcc gccaacatga tcggctccgg gtttctcggc gcggtcgttg
    13921 gcggattgat agccggttac ttgatacgct ggatgaaaaa tcacttgcgt cttagcagta
    13981 aattcaatgg cttcctgact ttttatctct acccggtgct cggtacattg ggagcgggca
    14041 gtctgatgct gtttgtggtg ggggaacctg tcgcctggat caataactcg cttaccgcct
    14101 ggctgaacgg tctgtcagga agtaacgcgc tgttgctggg tgccattctc ggttttatgt
    14161 gttcctttga ccttggaggg ccagtgaata aagccgctta tgcattctgc ctgggcgcaa
    14221 tggcgaacgg cgtttacggc ccgtatgcca ttttcgcctc cgtcaaaatg gtttcggcat
    14281 ttaccgtaac cgcttccacg atgctcgcac cgcgcctgtt taaagagttt gaaattgaga
    14341 ccgggaaatc cacctggctg ttagggctgg caggtattac cgaaggggcg atcccgatgg
    14401 cgattgaaga tccgctgcgg gttattggtt cgtttgtgct gggctctatg gtaacgggcg
    14461 ctattgtcgg tgcgatgaat atcggccttt cgacacccgg tgctggcatt ttctcgctct
    14521 ttttacttca tgataatggc gcgggcggtg ttatggcggc aattggctgg tttggtgcgg
    14581 cattggtggg ggctgcaatc tcgactgcaa ttctcctgat ctggcggcgt cacgcggtta
    14641 agcatggcaa ctatctgact gatggcgtaa tgccataaac aaaaaggaaa cgacgatgaa
    14701 agcggtatct cgcgttcaca tcaccccgca tatgcactgg gatcgggagt ggtatttcac
    14761 caccgaagag tcacgcattc tgctggtcaa taatatggaa gagatcctgt gccgactgga
    14821 acaggacaac gaatacaaat attacgtact cgacgggcaa acggcgatcc tcgaagatta
    14881 tttcgcggtg aaaccggaaa acaaagaccg cttgaagaaa ctggtagaag ccggcaagtt
    14941 gattatcggc ccctggtata cccagaccga taccacgatt gtttctgcgg aatccatcgt
    15001 ccgtaatctg atgtacggaa tgcgtgactg cctcgcgttt ggcgagccga tgaaaatagg
    15061 ttatttaccc gattcctttg gcatgtccgg gcaactgccg catatctaca atggatttgg
    15121 cattacctgc accatgttct ggcgtggatg ttctgagcgc cacggtacgg ataaaaccga
    15181 gtttttgtgg caaagcagtg acggtagcga agtgacggcg caggtgatgc cgttgggtta
    15241 tgccatcggt aagtacttac ctgcggacga aaacggatta cgcaaacgcc tcgacagtta
    15301 ttttgacgtg ctggaaaaag cgtctgtaac caaagagatt ttgctgccga atgggcatga
    15361 tcagatgcca ttgcagcaaa atatcttcga agtgatggag aaactgcgcg aaatttatcc
    15421 tcaacgaaag tttgtgatga gccgctttga agaggtattt gagaagatcg aagcgcagcg
    15481 agataatctg gcaaccctga aaggggaatt tattgatggc aaatatatgc gcgtgcatcg
    15541 caccatcggt tccacgcgca tggatatcaa agttgcccac gcacgtattg aaaataagat
    15601 tgttaatctg ctggaaccgc tggcaacgct ggccaggacg ttgggttttg gataccacca
    15661 cggcttgctg gagaaaatgt ggaaagagat cttaaaaaat catgcccacg acagcatcgg
    15721 ctgctgctgt cgtgacaaag ttcatcgcga aatcgtcgcc cgcttcgaac tggctgaaga
    15781 catggcggat aatctgctgc gtttctatat gcgtaaaatc gccgacaaca tgccgcagag
    15841 cgacgccgac aaactcgtcc tgtttaacct gatgccctgg ccgcgggaag aagtgatcaa
    15901 caccactgtg cggctgcgcg ccagccagtt taatctacga gacgatcgcg gtcagcctgt
    15961 accgtatttt attcgccatg cccgtgagat cgatccaggc ctaatcgatc ggcaaatagt
    16021 tcattacggt aattacgatc cctttatgga gtttgatata cagatcaacc agattgtccc
    16081 ttctatgggc tatcgcacgc tttatatcga agcgaatcag cctggcaacg tgattgcggc
    16141 aaaaagtgac gctgaaggga tactggaaaa tgctttctgg caaattgcgc tcaatgagga
    16201 tggaacgcta caactggtag ataaagatag cggtgtgcgc tatgaccggg tgttgcaaat
    16261 tgaagaaagc tctgatgatg gtgatgaata tgactattca cccgcaaaag aagagtgggt
    16321 aattacttcg gcgaatgcga aaccgcaatg cgagattatt catgaggcct ggcagagcag
    16381 ggctgttatc cgctatgaaa tggcggtgcc gcgtaatctg caacaacgca gcgcaagaca
    16441 atccactggc agagtagatg tcgagttggt tgtcactctt agtcataaca acaggcgcat
    16501 tgatgtggat atcaaccttg ataaccaggc tgacgatcat cgccttcgcg tcctgatccc
    16561 tacatctttt aacaccgata gtgttctggc agatacccag tttggttcgc taacgcgccc
    16621 cgtgaacgac agtgcaatga acaactggca gcaagaaagc tggaaagaag cgccggttcc
    16681 ggtatggaat atgctcaact atgcagcctt acaggaaggg cgtaacggca tggctgtctt
    16741 tagcgaaggg ctacgtgaat ttgaagtcat cggtgaagag aagaaaacct ttgccattac
    16801 gttgctgcgc ggcgtgggct tactggggaa agaagatctg cttttaaggc ctaggcggcc
    16861 ttcgggaatt aaaatgccag tcccggactc acaactacgt ggtctgcttt cttgtcgcct
    16921 aagtttattg agttataccg gtacgccaac cgccgctggt gtagctcagc aggcgcgagc
    16981 atggctgact ccagtacagt gttacaacaa aatcccatgg gatgcgatga agctcaacaa
    17041 agccggattc aacgtgccgg aaagttatag tttgttgaaa atgcccccag tgggatgcct
    17101 gataagcgca cttaagaaag ctgaagaccg acaagaagtg attttacggc tgtttaatcc
    17161 ggctgaatca gcaacctgtg atgcgactgt tgctttcagt cgcgaggtga tttcttgctc
    17221 agaaacgatg atggatgaac acattaccac cgaggaaaat caaggttcaa atctatcggg
    17281 gcctttttta cccggccagt cacggacgtt cagttaccgg cttgcctgaa tagcaatcaa
    17341 accgaagcca catatgcgcg gccagattgt tgacaaaggg cgctttgttc atgccggata
    17401 cggcatgaac gctttattcg gtctacaaaa gcaggcaaat tcaatatatt gcagagatga
    17461 tgtagacact ggcaagcgta gcgcatcagg taattttgtg tttatcttca ctctcaagct
    17521 acgtatatgt ggctttattt ttaacaaaat aataaccacg ggtgagttaa ttataatata
    17581 attataagtt aattaaatgt taatatcggc gggggggaga tttatgcctt tattaataat
    17641 cctgaaactc tgcgtcgtat tagccagtga ccaaaaaaag aattaaggtc aaccgtgctg
    17701 tttttgcttc gtctcttttt atctttaatt gccaaccgaa actaatttca gtcttataat
    17761 tcacacattt taaacataaa tatcac
//
LOCUS       DAEBXN010000087        17646 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.087, whole genome shotgun sequence.
ACCESSION   DAEBXN010000087 DAEBXN010000000
VERSION     DAEBXN010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17646
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.087"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(1..673)
                     /gene="chbR"
                     /locus_tag="KJL99_002969"
                     /pseudo
     CDS             complement(1..673)
                     /gene="chbR"
                     /locus_tag="KJL99_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
     gene            complement(681..1031)
                     /gene="chbA"
                     /locus_tag="KJL99_002970"
     CDS             complement(681..1031)
                     /gene="chbA"
                     /locus_tag="KJL99_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HBD4437623.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(1090..2438)
                     /gene="chbC"
                     /locus_tag="KJL99_002971"
                     /pseudo
     CDS             complement(1090..2438)
                     /gene="chbC"
                     /locus_tag="KJL99_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707374.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
     gene            complement(2523..2843)
                     /gene="chbB"
                     /locus_tag="KJL99_002972"
     CDS             complement(2523..2843)
                     /gene="chbB"
                     /locus_tag="KJL99_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HBD4437624.1"
                     /translation="MEKKHIYLFCSAGMSISLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(3142..3480)
                     /gene="osmE"
                     /locus_tag="KJL99_002973"
     CDS             complement(3142..3480)
                     /gene="osmE"
                     /locus_tag="KJL99_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HBD4437625.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            3682..4509
                     /gene="nadE"
                     /locus_tag="KJL99_002974"
     CDS             3682..4509
                     /gene="nadE"
                     /locus_tag="KJL99_002974"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HBD4437626.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETRNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPINVFDDFWKK"
     gene            4739..5626
                     /gene="cho"
                     /locus_tag="KJL99_002975"
     CDS             4739..5626
                     /gene="cho"
                     /locus_tag="KJL99_002975"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HBD4437627.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLHSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(5586..6161)
                     /gene="ves"
                     /locus_tag="KJL99_002976"
     CDS             complement(5586..6161)
                     /gene="ves"
                     /locus_tag="KJL99_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HBD4437628.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFTADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGVCWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(6364..6849)
                     /gene="spy"
                     /locus_tag="KJL99_002977"
     CDS             complement(6364..6849)
                     /gene="spy"
                     /locus_tag="KJL99_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HBD4437629.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIHEIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(7179..8147)
                     /gene="astE"
                     /locus_tag="KJL99_002978"
     CDS             complement(7179..8147)
                     /gene="astE"
                     /locus_tag="KJL99_002978"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HBD4437630.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSVRHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(8140..9473)
                     /gene="astB"
                     /locus_tag="KJL99_002979"
                     /pseudo
     CDS             complement(8140..9473)
                     /gene="astB"
                     /locus_tag="KJL99_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
     gene            complement(9470..10948)
                     /gene="astD"
                     /locus_tag="KJL99_002980"
     CDS             complement(9470..10948)
                     /gene="astD"
                     /locus_tag="KJL99_002980"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HBD4437631.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLASLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVGGVPDEEVFGPFLRVWRYDTFDEA
                     IRMANNTRFGLFCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(10945..11979)
                     /gene="astA"
                     /locus_tag="KJL99_002981"
     CDS             complement(10945..11979)
                     /gene="astA"
                     /locus_tag="KJL99_002981"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HBD4437632.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(11976..13196)
                     /gene="astC"
                     /locus_tag="KJL99_002982"
                     /pseudo
     CDS             complement(11976..13196)
                     /gene="astC"
                     /locus_tag="KJL99_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
     gene            13642..14448
                     /gene="xthA"
                     /locus_tag="KJL99_002983"
     CDS             13642..14448
                     /gene="xthA"
                     /locus_tag="KJL99_002983"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HBD4437633.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGVVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            14615..15325
                     /locus_tag="KJL99_002984"
     CDS             14615..15325
                     /locus_tag="KJL99_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBD4437634.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFINAPLFLLPGSILVIAGGIVFGPLLGTQLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGFTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILIQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            15330..16007
                     /gene="ydjY"
                     /locus_tag="KJL99_002985"
     CDS             15330..16007
                     /gene="ydjY"
                     /locus_tag="KJL99_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HBD4437635.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYAPPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKHGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            16022..16729
                     /locus_tag="KJL99_002986"
     CDS             16022..16729
                     /locus_tag="KJL99_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBD4437636.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTELFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            16729..17277
                     /locus_tag="KJL99_002987"
     CDS             16729..17277
                     /locus_tag="KJL99_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HBD4437637.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            17287..17613
                     /locus_tag="KJL99_002988"
                     /pseudo
     CDS             17287..17613
                     /locus_tag="KJL99_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         4366 a         4687 c         4307 g         4286 t
ORIGIN      
        1 tctcattaat aatctgcatt ggtgttttgc cataatatcg ttgagtcgct cgcgtcaaat
       61 attcctgtga tttggctgac aacgtcacca tattctccag cgccgattca ctaaactgct
      121 ctttatcatg catcttttct accgtacttt tcagccacta cggtacatca tcaatcacct
      181 gttcttcgcg gtaatgacgt aaacggttaa tgacataaaa agtaaccatc tcaacaaact
      241 cttccagccc tgtttcgcgg aaatttaatg aagaaatcac tgtttccaca taggtgagaa
      301 aagcgttatt ggtccggtat acctgcgaag caacaaagca ataaggcaac aacggcaggt
      361 aatgctgctc aaaaaagcgt ttactgatcc caacgttcaa tatgcgcgtg gcaccaaact
      421 cataaaaact ttgatggtgc gaacctaacg gaataaaaac aaaatcgccc cgttccagta
      481 acacgcgctt accgttaatc tcctggaaat aacgcccggt taataccaga gtaaattcat
      541 aatagtcgtg ctgatgcagt ccgctgatac tctcagtttt gttatagata aacacatgga
      601 agtttttgcc attaaacaac tgctgttctc gggcagtggc aatttccggc gcgttaatca
      661 ctggctgcat catcgactcc ttatgccttc agtttttcat gaagctcaat taattcagta
      721 atcagttcac gcgcaagcat ggacgtcatt aaatgatcct gagcgtggac cagcaccaga
      781 ctcactttca tcttaccttc gcccgcatcg ccttcaatca gtttcgtctg taccagatgc
      841 gcttcattca atgccatccg tgactgatcc atcatggctt tcgctgcggc aaaatcgccc
      901 tgcttcgcct gtttcaatgc ggcatacgcc aggctacgcg cttgtccaga gttgatgata
      961 agccccatca ccacttcttc cagctcttca gcttctgttt gcgtatcagg aatattatcg
     1021 agatccatca tacatcgttc ctctctcttc ttaccggcaa gattacccgt accggcatcg
     1081 attaaaattt caggggttag cgatatcttc ttcgctctct tctttatcaa tcgcattttg
     1141 tgctttgtta gccaccacaa caaagggcag ataaattaac gttgcgatgc caaggttgaa
     1201 gagtgcgacc agcaatgcgg cgacgctacc gttggtgtta aagaaggctc ccagaccggt
     1261 tggcatggtc cacggtgcaa tattggtcac cggaggaatg atgcccatgt agtacgctgc
     1321 gagggtgatt gccgccagaa tcggttgtac cagtacaaac gggataaaca tcaccgggtt
     1381 catgataatt ggcagaccaa acagaatcgg ttcattaatc tggaagatgc cggacggcag
     1441 cgccagtttt gccacctgac gataatcagc acggcgagag gcgataaaga tagccaggat
     1501 caggcctaaa gtcgcaccac tgcccccaag gaaaataaag gagtccagca tcggcttggc
     1561 ccagatatgg aaggtcttac cggctgccag cgccgcttcg acggaaccat attgctgata
     1621 ggtcgcgata ttttccagcg cccacggcgt cataatgccg ttgtccagtg cggtcagcgc
     1681 cagcgcgcca tgaataccga agaaccagag cagtggaaca aagatcacat aggcccagcc
     1741 caccacgcta cccaacgatg ccagtggggt tgagatggta tccataatga tctgatggaa
     1801 gttggtgccc caggtattca acgcccaggc aataatcccc atcacggaaa gaataataaa
     1861 gccgggaatt aatgccgaga aagaacgcga tactgaagca ggtacgctgt cgggcagttt
     1921 aatgacccaa ttgcggcgga caataaaggt aaacatttct gccaccacca ggccaataat
     1981 aatcccggag atgatattcg ccccacctaa ccagtttgcg ccaaccgcat aggcctcacc
     2041 gacactatat ggggtgacgg tcataaatgc tgcaacggat aacaacccag ccgccagcgc
     2101 atcgacttta cgctcttctg ccagcgccat gccaataaag aacggtgcca ttaaagacat
     2161 tattcctaat gttccgttat atacgttgcc gccaatacct ttcagaccat taagtgtttc
     2221 aatggttgag gcgtcgaggc gaatacctaa ggaataaaaa acgacccctc cccaaagctt
     2281 agaaaaacgt tgttaattaa tacaaacatg gcccccgcaa gggttaacgg cattaagcga
     2341 ataaagccat ttttgattgc attaacgtgt ggctgctttc ctattttaac tgcaaaaggg
     2401 aggagtacct tttcaagcga tgcaataaca ttactcatag aaaaataccc ttaaaaaccg
     2461 caatttaaat attacggtat tgatttatga aataactctt tgacgggaaa atttaaaata
     2521 aattaatttg ctgcggcttt tttaatcgct gcaaccgcag ccttaagcac gcctaaacca
     2581 tcgactttgc cataaagcag cgagtcaatt acttcaaccg gtttgttggg taacaaacgc
     2641 tggatttcgg gcaacatata agcaatctgc ggccctaata acacgacatc ggcattctga
     2701 cctttttcac cagccagtgt ttccggaaat gcttcaataa tgaccggaac ttcatatttt
     2761 tctgcctgtg cgcgcatttt tgataccagt aaagagatag acatgcccgc agaacaaaac
     2821 agataaatgt gtttcttttc cataaaactg ccctcatcga cgattatctg tcagccagac
     2881 actccgcaag ccttgacctg cttccatgct ctgggtaact tgcgaaacca acatgacttt
     2941 ttttgtggct gaaaggagta tacggtatag acctgagcaa tagtatttct ttgaccttat
     3001 aaattgtttc tccttgacct gtggttatga ccctcttcac atttcgggca aataattcgc
     3061 gaagataatt aagaactcag gccaaaaaaa accggcgcaa tggccggttt ccgttgttac
     3121 tcaagctttc agacgaattg attacttcgc agcctgtgga tcagtgtcgt attcagcaca
     3181 ggtctgataa ccggagttga tgacatgtcc ggtatcatct aacgcgacaa agtaggtttc
     3241 tgctttacca tcacgttgac ccaggatgta ggtctggcaa gtaccgcgag catggatcat
     3301 gctcacttca gacgaaggtt tacccgcaat ttgtgcaacc tgcgcccggc tcatgccttt
     3361 tttgacgtct ttcaccacag gctgtacaaa ctggtctttg gtacgatcat aagctgtaca
     3421 acccgccagc atggttaata ccgccgctgc actcagaatt cctgccatat tcttgttcat
     3481 attccgtcct cttgtttatc agcgtgttag ataagcctgg aatacattgg gcgctttttc
     3541 aagcccgtga acgaaacggc tccgctttca gaggattcct gtatgacgtt ttaaccacca
     3601 ttcagcccgc tgtcgcttgt cgtttcagta gcaacgggtt agctttaagg aagttttgtc
     3661 ttttctgtct ggaggggttc aatgacattg caacaacaaa taataaaggc gctgggcgca
     3721 aaaccgcaga ttaatgctga agaggaaatt cgtcgtagtg tcgattttct gaaaagctac
     3781 ctgcaaactt atccgttcat taaatcactg gtgctcggga tcagcggcgg tcaggactcc
     3841 acgcttgccg gaaagctgtg ccagatggcg attaatgagc tgcgccagga aactcgcaac
     3901 gaatcactgc aatttattgc cgtacgcctg ccctatggtg ttcaggctga cgaacaagat
     3961 tgccaggatg ccattgcctt tattcaaccg gatcgcgtat taaccgttaa tatcaagggc
     4021 gcggtattgg ctagcgagca ggcattgcgg gaagcaggca ttgaactgag cgattttgtc
     4081 cgtggcaatg aaaaagcgcg tgagcggatg aaagcacaat atagcattgc gggtatgacc
     4141 agcggtgtcg tggtgggcac cgatcatgca gcagaagcca ttaccggatt cttcactaaa
     4201 tatggtgacg gcggtacgga tattaatccg ctgtatcgtc tcaacaaacg ccagggtaaa
     4261 cagttactga cggcattagg ttgcccggaa cacctttata agaaagcccc aactgccgat
     4321 cttgaagatg atcgcccttc tctgccagat gaagtggcac tcggcgtgac ctatgacaat
     4381 atcgacgact atctggaagg gaaaaaccta cctgaacagg tcgccagaac aatagagaac
     4441 tggtatctga aaaccgaaca taaacgccgt ccgccaatta acgttttcga tgatttctgg
     4501 aaaaagtaat aatttgcacc tattggattg tgcgaaaaag agtaatttgt tcacgccgga
     4561 tgcggcgtga acgccttatt ctacctacaa aactatgcaa attcaataca ttgcaggagt
     4621 cgaataggcc tgacaggcgt agcacgtcag acggtgtaac ctttgtcatc aacccgcctc
     4681 ttttttaatc gcctcccgcc tgttacactg gatagataac cagcattcgg agtcaacagt
     4741 ggtacggcgt ttaacttctc cgcggctcga atttgaagct gcggcaattt atgaatatcc
     4801 cgaacattta cattcattcc ttaatgactt acccacccga ccaggggtgt atctgtttca
     4861 tggcgaaagt gacaccatgc cgctctatat cggcaaaagc gttaatatcc gcagtcgggt
     4921 gctttctcat ttacgcaccc cggatgaagc cgccatgcta cggcaatccc ggcgaattag
     4981 ctggatctgc accgcaggcg aaatcggcgc tctgctcctt gaagcgcgat taatcaaaga
     5041 acaacagccg ctgtttaata aacggttgcg ccgcaatcgc cagctctgtg ccctgcaatt
     5101 aaatgaaaag cgcgtcgatg tggtgtatgc caaagaggtg gatttttcac gagcccccaa
     5161 cctgtttggc ctgtttgcca ataggcgcgc agctttgcaa gcattgcaga gcatcgctga
     5221 tgaacaaaaa ctttgttatg gcctgttggg actggaaccg ttaagtcgcg gtcgtgcatg
     5281 ttttcgttca gcgctaaaac gttgcgccgg agcatgctgc ggtaaagaga gccatgagga
     5341 acatgcgcta cgcttgcgcc agtctctgga gcgtttgcga gtggtgtgtt ggccttggca
     5401 aggtgcggtg gcactgaaag aacagcaccc ggaaatgact caatatcata ttattcaaaa
     5461 ctggctgtgg ctgggggcgg ttaattcgct ggaagaagcg acaacgttaa ttcggacacc
     5521 cgccgggttt gatcacgacg gttataaaat tctttgtaag ccgctgcttt ccggtaacta
     5581 tgaaattact gaacttgatc cggcgaatga ccagcgagcc agttgatttc actgaacaac
     5641 agtttacctt gcggttgtaa aagccgcagg gtgtgcctgc catcgaacca gcacacgccc
     5701 tggtcggtag tgagcagttt gtcgcccagt tgccacgccc cgttaatcac aaacaccacc
     5761 ccgccgcgtg agccaaaggt agtaaacgta cgttcggcaa ttctgacttt cgccttacag
     5821 acatccaggc gcgtcataat attgaaatcc atcgacatct gccctgccgt cagtttcgct
     5881 tttaccacct ggtccgccgt aaaggcaaaa ggctgtaacg gttttaaggt atggttaaag
     5941 cggtctgcgc tttcaaggag catctcgccg ccttccagca acgtcactat cctttccatg
     6001 ccgggaaaca aagaaaactc cccattcgcc gcgatggatg caatgctggc acgccagtaa
     6061 aaatcacgtt ttgccggtgg gaaagtgcaa atttcgcgcg tttcaccggc agcgtttcgc
     6121 cacagattca ccgacatttt acgcatatca aagtattcca tactcgctcc cggcccgttc
     6181 ttttcattgt tatcgttcag gtaaccgtgg atggttatcg gcaactcggg tgatttacgt
     6241 tagtggtgat caggcttttt ttacaactgt ttgagaagag aaaagaaaac cgccgatcct
     6301 gtccaccgca ttactgcaag gtagtggaca agaccggcgg tcttaagttt tttggctgaa
     6361 agattattca gcagttgcag gcattttacc ttttgccgct ggacgttctg tcagacgctt
     6421 ctcaaaatta gcattaaatt gctttttctg ttccggcgtc aggatgttgt aaattttgtt
     6481 ctgggtttcc atgtgcgcca gcatgttagc tttgcgctgt tcttccattt ttgcgatctg
     6541 cgcttcagct tttgctttat cgaaggtatc gctggcaatg atgtcatgca ttgcgcggcg
     6601 ttcttccagc ggcggacgtt tcatctggtc acgctggcct ttcatgattt cgtggatctg
     6661 ctgtttctgc gcgtcggtca ggttcaggtc tttgaacatc atgtcctgat gcggaccgaa
     6721 cttgcctttg tggtgcatca tcggcttcgc gtcagccggt gctgcggtag tggtgtctgc
     6781 ggcatgggcc aggttagccg cgccaagagc cagggtagag gcaacaaaca gtgcagttaa
     6841 tttacgcata ttctatatcc ttcctttcag ttatttatta cggctttctt aagtagcgtg
     6901 ccgtgttgac gagattaact ttactgactt tagcgtcaat taatcagagc aacggtaaaa
     6961 caatgaaagt gtaaaaaaca cttttgcgcc aattatggag aaaaaaagaa aatttgatgg
     7021 agagtgatga gagaatatta caacacgatg attttgcaga gattatgaag aaccataccg
     7081 gatgactggt gataaataaa gcaaataacc aggattaatc tgtattaatt tataagaaag
     7141 caacttaata cccgcagaat gatttctgcg ggtaagtatt agcttatttt ttcgagcatt
     7201 aatcccgcgc gtaatcccaa cgctaccaac ggattaggga ataacacata ctctacatca
     7261 tgggttacgg taaaacgttc ctctccgtcc tgcgccagca atgttccttt ctcaaacggc
     7321 ataaaattca gcgtgtcact tgccatatgc atttcgaagg acggcgagtg gcgagtaatt
     7381 tgcgaaacca cccgataacg gagcggcggt gttctcacga taccgacact ctcaccagat
     7441 agcagcgcag caattgcgct ggcagttact gcaaactggc gaagatcgtt ttgcccaaag
     7501 ggcaacgctt tgccaagttc caacgtacag gccagcgcgc caaaatgtct gacgctgaaa
     7561 tgggtaaacg taccaccagg ctcctgatgg aacaccagcg cctccagccc cgccgcaccc
     7621 agccacgtca gaaatttctc gtcccaggga atgtcgcgtt gcggtaatac accgaactgc
     7681 ggatgcaagg agccacgaat tgcggtatgt agatcaaggt gccaacgcac agattctttg
     7741 ccctggtcat aaaaatcttc caggcactgt tccagttcgc gcgcccgaca ggtttctccg
     7801 ctttcagcaa atagctgcca acgaccgcca aacattcggt tcatatcgct atggcaataa
     7861 cgtttccctt gcttcagcgc aggaggattc ccgaggatca ccagcaaccg ccaacgtaac
     7921 gggatctcgc cgtgagatat cgcgccaagc aacgcgtcca gcatttccac aggtgccgtc
     7981 tcattaccgt ggatccccgc agaaatcacc agcgcgcctt gcggtggcgt taatggcgtc
     8041 agttccagca caccatcgcc cagccagcgc cagcgaacgc cgttgatttc tcgctcggtg
     8101 ataaccggtt ttttacccgt taaggtcaga gcaagaaaat tatccattgc cccctccctc
     8161 gcgctggaac ggataaaccg aaccgagatt cagtaattgg ctcaatacat ccagtgcttc
     8221 ccgcccttcg cgcagcaatt gcgggtcggc cagatcggca gcagtaagac gatcgcggta
     8281 gtaacgatcc acccagtcat tgagcgcatt aaacaaggta tcgttcatca tcaccgccgg
     8341 attcaccgcc cggcgttctt cttctgtcaa taccacccgc aaccgcaggc acgccgggcc
     8401 gccgccattc gccatgcttt cacgtaagtc aaagactttt agttcgctaa tcgggttgtc
     8461 agcggcaagg agttcattga gataacccca tactccggcg tgttcccgac actcctgagg
     8521 cagcaccaac atcatggaac catcatcgcg gctcaacagt tggctgttaa acagataggt
     8581 agacaccgca tccgacacgg aaacctgagt tgccggaact tctatcgcca taaaaccgtt
     8641 cacccgcgtg ccagtaactg tgactggcga gcgaacgcct gttggtggca aaacagcacc
     8701 tggcggttac tcacggcaat cacgtcatta tgaaaaacgc tctggtcgat aacgtccggg
     8761 ttttgctggg cgaaaatcac ctgttgggga ttcacctgat taagccttgc caccgcctcg
     8821 ctggcttcgc gagtctgtcg cgccggataa cgggaaggcc gggtatcatt gccttcttct
     8881 cgcccgtaga caaaaagtag catacccggt tcaccgtaat gaccgccgag acgattgtgg
     8941 tttgccgccc cctcatcacc gagcaacgct acctgcggca acgccgaatg gacgctaaat
     9001 ttctcttcgt ccttaaaaat cgcttttaac agcgattcag tgacgggcgc ttccagcgaa
     9061 cggtgaaatt tattgttcag gttggcaacg gtgagatgca ctttgccatc cagcgtatcg
     9121 gcagatggcg cgatcgttgc cgcattggct acccacattg gcgaagcgga actgacgctg
     9181 gaaagccagt gcggtgcctg gcgtgcaact ttttccagta cctgctcatc gctaccactg
     9241 aatcccaact gacgcagcac cggaataaac ggacgctcgt gcggcggaat cacggcctgg
     9301 gggaatcccg catcggcaag ggctttcatt ttcagtaagc cctgttttgc cgccagtcgc
     9361 gggttagaca cctgaaaacg gtgacgggta gaggcttcat taccaaacga caggcccgcg
     9421 taatgatgcg tcagccctac cagcccgtcg aaattaactt cccaggcgtt catcgcacca
     9481 cctcatcgga aaaatccagc ccggggttaa gcgtggcggg caatgttaac gagtccgact
     9541 ccaggctcgc cataggccat gcgcagtaat ctgcggcata ccaggcgctg gggcgatggt
     9601 taccggaagc accaatgccg ccgaatgggg cggtactggc agcaccagta agcggtttgt
     9661 tccagttaac aatccccgcc cgcgcctcca gcaacagttg atcgaatttt tcccgctcgg
     9721 gggaaaccag accgcaaaag agtccgaagc gagtgttgtt cgccatccga atcgcctcat
     9781 caaaagtatc ataacgccag acgcgcaaaa acggcccgaa cacctcttca tccggcacac
     9841 cgccaacgcc cgtcatttca atgatccctg gtgtcagcaa cgatgtccct gcttgtaata
     9901 agcgcggcgc aagcagggtt cgtccgccca tcgcttccag ttgctgccag gcagtaacca
     9961 cctgctgtgc ggcctgttca gaaatcagcc cgccaataaa cggctgcggt tcgtcatccc
    10021 agttgcccgg cgttaatcgc tggctgacgg caaccagact agcaagaaac gcatcgccct
    10081 gcgctccgct tttgagcaat aaacggcggg cgcaggtgca gcgttgcccg gctgtgacaa
    10141 acgccgactg aatggtcaga tggacagccg cgtcgatatc cgccacctca tcgataatta
    10201 gcggattatt accgcccatc tcaagggcga gaattttctc cggctgaccg gagagctggc
    10261 gatgcagctg gtagccggta ttggcgctac cggtaaacag caaaccgtcg agatcctcca
    10321 gcgcactcag cgcctgaccc gtttcacgcc cgccctgcac caggttcagc acgcccggcg
    10381 gcaagccagc ctgctgccat aaacgcatta ccgcttcgcc actccacggt gtcagttcgc
    10441 tgggtttaaa gataatggtg ttacctgcca gcaatgccgg aacgatatgt ccgttcggca
    10501 aatgaccagg gaaattatac ggcccaaaca ccgccagcac gccgtgcggg cgatgtcgca
    10561 ggctcgccgc accgtccggc atttcactac gctgctcgcc ggtacgaacg tgatacgcct
    10621 taattgatat cgcgatttta ttgatcatcg ccgtcacttc ggttgccgct tcccagcgcg
    10681 gcttacccgt ttctctggca ataatcgcgg ttaattcggc tttattgctt tccagcaatc
    10741 cggcaaagcg ttcgacaacg acctgacgtt cagccaatga gagccgcgcc cagcgcggaa
    10801 acgccgcacg ggctgcccga caagcctgtt cgacctgagc ggcatcggca tcattgcctt
    10861 gccataacac ctcgcccgat accggattac gcttcacacg cgatgcgccc tggcccgtta
    10921 tccagtcacc gttaatccat aaagtcatgc tgttttctcc tctgcgcaca ggcgcaccag
    10981 acgaacgcga tcccctgcgt ggcatttgag ggcatccagt tgtgcggcgg ttaaaatcaa
    11041 acgctcggtt gccggatcgg tacgcgccag caccacgcgg aaatggtgat aattttcatt
    11101 ggcgaccagg caggccggaa aatcgccctg cgcaggctgc ccttctgcca cttccaccag
    11161 ccgactttta cggatggcgc gcacgcggtc gatgtcacac tcaagcgttg gcccgccgtc
    11221 gaagatgtcg atatagttgc ggtaacgaaa accttctttc tccagcaccg cgcgggcagg
    11281 cgcggtttgc ggatgtacct gaccgatgac gtcctgggct tcctgggata aaaagtgggt
    11341 atagatcgga tgtttcggca tcagttctgc aataaatgcc ttttgcccgg tgccgcagag
    11401 aaaatcggcg cggctaaaat ccatcgaaaa gaagcgttta ccgaggcttt gccagaacgg
    11461 tgaatagccg tgttcgtcaa tcaccccgcg catttcagca accactttgt cattaaactt
    11521 gtcgcgaaaa gccgccataa acataaagcg cgatttcgat agcaaatagc cgttgccctc
    11581 tttgcgccag tccgggtcga gaaacagcgt gcacagctcg ctgctgccgg tgtgatcgtt
    11641 actaagaaac agcgtcggca atgcgttata gacattcagc tcttttgagg cgtgaaccaa
    11701 cgtgccgacg cgatagttgt accagggatc gttcagccca accgccacct caatagcgca
    11761 aatccccgcc acggtgcctg tctcgctatc ttccagcacg aacacatagc cctgctcgct
    11821 tttgggcagt tcaccttgcc aggttttgat tgccctttcg atacgcgccg aaagcgtggc
    11881 ttcattggcg ggaagcgacg tcaggccgcc gcccgttttg ctggcaagct gcatcagcgc
    11941 cgagacatct gagcgctcaa cgggacggat gaccatcatg atgaacctcg gctaacaaag
    12001 tgttcgcaag cagctgcaaa gcgatccagt ccggtcgtca cctcttcttc gctgacattg
    12061 agcgcaggcg caaaacgcac cacgttgcca cccgcaatca gtaccatcac gcctgctttc
    12121 gccgcttcca gagagatctg tttcgcttgc ccggcgtaat cggcattcag tacacagcca
    12181 atcaacaaac ctaagccgcg aacttcactg aacaaaccat agcggtgatt aatagtatta
    12241 agacgctcaa caaaccagtc gtgacgctgt ttaacgccat taagcatctc tggtgtgttg
    12301 atgagctcca gcactttgcc tgctaccgcc gaggccagcg gattaccgcc ataggtggtg
    12361 ccatgagtgc caacggtcat cacgcgggcg cactcttcgg ttgccaacaa cgcaccgacc
    12421 gggaaaccgc cgcccagcgc tttggcggtg gttaacagat cgggcgtcac gccgtagtgc
    12481 atataggcat acagctcacc agtgcgcccg acgccggttt gtacctcatc aaaaatcagc
    12541 aaggcattgt ggcggtcaca caattcacgc agaccttgta aaaacgcgtt gctggctggc
    12601 accacaccgc cttcgcccta gatgggctcg acaatcaccg cacaggtggc gtcgtcaatc
    12661 agcgcgctgg cagagttaat atcgttatat gcagcatgac gaatatccgc cggcagtggc
    12721 gcaaaatcct gtgaataggc tggctgccca cccgcactga cagtaaacag cgtgcgacca
    12781 tgaaacgcat ttttgaacgc cacgatgcca ctcttatggc tgccgtagcg gtcgtgagcg
    12841 aatttacgcg ccagttttag cgccgcttcg ttggcttccg caccggagtt acaaaagaag
    12901 acgcgatcgg caaacgtggc gtcgatcaat tttttcgcca gtcgcagtac cggctcgttg
    12961 gtgtaaccgt tgccggtatg ccagaactta ctcgcctgtt cgttcagcgc ttcacgcagt
    13021 tccggatgcg catggcccag cgcgttcacc gcaatgccac ccgcgaagtc gatatactct
    13081 ttcccctgct gatcccacaa gcgcgaacct tcgccacgta ccggtataaa gggtgccgga
    13141 gcgtaaacag gtatcatcca ttcatcaaag ttttcacgcg taattggctg agacatagcg
    13201 acctctacag taaataattc gttatttgta tgttaacaat aagtaatgtt tgcgctgtaa
    13261 atgtagattg cagggttcgt gccagccagt gataaaagtg cataaacggc ggaggctaac
    13321 tggaaatcaa ggagttataa ccaaaccata tgcatttaaa gtgcatataa agtgaatacg
    13381 tttgcgatgt gggtgaataa aaagaataaa aaacgcaatg ttatgcagaa gtaaaatata
    13441 attctagaat tgtgatcatt gacgaaattt actggaaatt actgcgccat tctgacgcag
    13501 cgcgcaccaa aagcgggcat tttttgcgcc atcgttgaca tcattaacaa ccatcgatca
    13561 aatcacttaa caacaggcgg taagcaacgc gaaattctgc taccatccac gcactcttta
    13621 tctgaataaa tggcagcgac tatgaaattt gtctctttta atatcaacgg cctgcgcgcc
    13681 agacctcacc agcttgaagc catcgtcgaa aagcaccaac cggatgtgat tggcctgcag
    13741 gagacaaaag ttcatgacga tatgtttccg ctcgaagagg tggcgaagct cggctacaac
    13801 gtgttttatc acgggcagaa aggccattat ggcgtggcgc tgctgaccaa agagacgccg
    13861 attgccgtgc gtcgcggctt tcccggtgac gacgaagagg cgcagcggcg gattattatg
    13921 gcggaaatcc cctcactgct gggtaatgtc accgtgatca acggttactt cccgcagggt
    13981 gaaagccgcg accatccgat aaaattcccg gcaaaagcgc agttttatca gaatctgcaa
    14041 aactacctgg aaaccgaact caaacgtgat aatccggtac tgattatggg cgatatgaat
    14101 atcagcccta cagatctgga tatcggcatt ggcgaagaaa accgtaagcg ctggctgcgt
    14161 accggtaaat gctctttcct gccggaagag cgcgaatgga tggacaggct gatgagctgg
    14221 ggggtggtcg ataccttccg ccatgctaat ccgcaaacag cagatcgttt ctcatggttt
    14281 gattaccgct caaaaggttt tgacgataac cgtggtctgc gcatcgacct gctgctcgcc
    14341 agccaaccgc tggcagaatg ttgcgtagaa accggcatcg actatgaaat tcgcagcatg
    14401 gaaaaaccgt ccgatcacgc ccccgtctgg gcgaccttcc gccgctaatt tagcagctct
    14461 cctggctcaa actgggtcag gagaattaac cttgagaaaa atcaacaaac tgtcagtaat
    14521 gatttgttgc ctgccgtcct ttgttatacc gtcactgcgt ttttagttgt ctgaccactt
    14581 ctctattatc aagtttgata taggaaactc cacgatgaac gctgagcgta actttctgtt
    14641 tgcctgtctt atttttgcgc tggtcattta cgctatccac gctttcggtt tattcgatct
    14701 gctcaccgat ttaccccact tacagacact cattcgccag agcggattgt tcggttatag
    14761 cctctatatt ctgttattca tcaatgcccc cctctttctg ttaccaggaa gcatactggt
    14821 gatcgctgga ggaatagttt ttggcccgct cttagggaca caactctcat taattgccgc
    14881 aacgctggcc tcctcgtgct cattcctgct ggcgcgctgg ctgggacgtg atttactgct
    14941 gaaatacgtg ggccatagcc ataccttcca ggccattgaa aaaggcattg cgcgtaacgg
    15001 tattgatttt cttattctga cccgcttaat cccgttgttt ccttacaata ttcaaaatta
    15061 cgcttacgga ttcaccacca tcgccttctg gccttatacc cttatttcgg cactcacgac
    15121 cctgcccggt attgttattt ataccgtgat ggcaagcgat ctcgccaatg aaggcattac
    15181 gctgcgcttt attttacaac tctgtctggc gggcctggcg ctgtttattc tcatccagct
    15241 cgcaaaactc tacgcccgac acaaacatgt ggatctgtct gcttcgcgcc gcagcccact
    15301 tactcaccca aaaaatgaag gatagaacga tgtcgcaaca ttattcagtg tcatggaaaa
    15361 aaggactggc tgcactctgc ttactggctg ttgccgggct tagcggctgc gatcaaaaag
    15421 agaatgcggc ggcaaaagtg gaatacgacg gactttcgaa cagccaaccg ctgcgcgtcg
    15481 atgccaataa ccatacggta accatgctgg tgcaaattaa tggtcgtttc ctcaccgacg
    15541 acactcgtca cggtattgtg tttaaagatg gctccaacgg acataaatcg ctgtttatgg
    15601 cttatgcgcc cccgaaagca ttttatgaag ccctgaaaga ggcaggtggt acgccgggcg
    15661 aaaacatgac gatggataat aaagaaacga ctcatgtcac aggcagcaaa ctggatattt
    15721 cggtcaactg gcaaggggcg gcaaaagcgt attccttcga tgaagtgatt gttgacagta
    15781 atggcaagaa actggacatg cgctttggcg gtaatttaac ggcagcagaa gagaagaaaa
    15841 cgggttgcct ggtgtgtctg gatagctgcc cggtcggcat cgtcagcaat gcaacataca
    15901 cttatggtgc ggtggaaaaa cacggcgaag tgaaattcaa aggcaatgcc tcagttctcc
    15961 cggcggataa cacgctggca acggttacct ttaaaatcac cgaataaagc caagataaag
    16021 gatgatgaag atacaatcgc gtaaaatctg gtactaccgt ataaccctca tcatcctgtt
    16081 gttcgctatg ctgctcgcat gggcgctgct tcccggcgtc catgagttta tcaatcgcag
    16141 cgttgcggcg tttgccgccg tggaccaaca gggtatagaa cgctttattc agtcttacgg
    16201 cgcactggcg gcagttgtct cgttcttgtt gatgatttta caggccattg ctgcaccgct
    16261 acctgcgttt ttgatcacct ttgccaatgc gtcgctgttt ggcgcgttct ggggcggctt
    16321 gctgtcgtgg accagttcga tggccggcgc ggcgctgtgc ttttttatcg ccagagtgat
    16381 gggccgcgaa gtggtggaaa aattaaccgg caaaaccgtg cttgacagta tggacggctt
    16441 tttcactcgc tacggcaaac acaccatcct ggtctgtcgg ttattgcctt ttgtcccttt
    16501 cgatccaatc agctatgctg ccggtctgac ttcaatacgt tttcgctcgt tttttatcgc
    16561 caccgggctt ggtcaattac cggcgactat tgtttattcc tgggcaggca gcatgttaac
    16621 aggcggtact ttctggtttg tcaccgaact gtttattctg tttgccctga ccgtggtgat
    16681 ttttatggcg aagaaaatat ggcttgagcg ccagaagagg aatgactgat gggtttaccg
    16741 ccgcttagca aaattccttt cattttacgt ccgcaggcgt ggctgcatcg tcgccattac
    16801 ggcgaggtgc taagcccgat tcgctggtgg gggcggatcc cgtttatctt ttatttggtg
    16861 tcaatgtttg ttgggtggct ggagcgcaaa cgctcaccgc tcgatccggt agtgcgatcg
    16921 cttgtcagcg cgcgcattgc gcaaatgtgc ctgtgtgagt tttgcgtgga tatcaccagt
    16981 atgaaagtcg ccgagcgtac cggcagcacc gataaactgc tggcagtggc tgactggcgg
    17041 caaagcccgc tctttagcga tgaagaacgg ctggcgctgg agtacgccga agccgcaagc
    17101 gtcacaccgc caacagtcga tgatgccctg cgtacccgac tggctgcgca ttttgacgct
    17161 caggcgctca ccgaactgac ggcattgatc ggcctgcaaa acctgtcggc tcgttttaat
    17221 tctgccatgg acattcccgc tcaggggctg tgccgtattc cggaaaaacg ttcttaagga
    17281 gggaagatgc gccattgtgg gtggttgctg ggattgttat cgctgttttc tttggcaaca
    17341 catgccagtg actggcagga aattaaaaat gaggccaaag ggcaaaccgt ctggtttaac
    17401 gcctggggcg gcgataccgc aattaaccgc tatctcgact gggttagcgg cgagatgaaa
    17461 acccattacg ctataaacct gaagattgtt cgtctcgcgg atgccgcaga cgcggtgaag
    17521 cgcattcaga ccgaagctgc tgccggtcgt aaaacgggcg gctcggtgga tctgctctgg
    17581 gtgaacggcg aaaacttccg caccttaaaa gagtaatgcc agtcagttaa gcaactgact
    17641 ggcatt
//
LOCUS       DAEBXN010000088        17548 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.088, whole genome shotgun sequence.
ACCESSION   DAEBXN010000088 DAEBXN010000000
VERSION     DAEBXN010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17548
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.088"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..185
                     /locus_tag="KJL99_002989"
     CDS             <1..185
                     /locus_tag="KJL99_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HBD4437638.1"
                     /translation="EWQKDVDLHQVLWSRSRLGERPKGQGITGADHFWFGHTPLRHRV
                     DIGNLHYIDTGAVFGG"
     gene            complement(210..401)
                     /locus_tag="KJL99_002990"
     CDS             complement(210..401)
                     /locus_tag="KJL99_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HBD4437639.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGSCF
                     PIEDFIDGFWQPAQEYGDF"
     gene            complement(506..742)
                     /locus_tag="KJL99_002991"
     CDS             complement(506..742)
                     /locus_tag="KJL99_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HBD4437640.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(860..2299)
                     /gene="rsmF"
                     /locus_tag="KJL99_002992"
                     /pseudo
     CDS             complement(860..2299)
                     /gene="rsmF"
                     /locus_tag="KJL99_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
     gene            complement(2379..4999)
                     /gene="yebT"
                     /locus_tag="KJL99_002993"
                     /pseudo
     CDS             complement(2379..4999)
                     /gene="yebT"
                     /locus_tag="KJL99_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
     gene            complement(4968..6251)
                     /gene="yebS"
                     /locus_tag="KJL99_002994"
     CDS             complement(4968..6251)
                     /gene="yebS"
                     /locus_tag="KJL99_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HBD4437641.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYRRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGLCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSILDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            6381..6878
                     /gene="msrC"
                     /locus_tag="KJL99_002995"
     CDS             6381..6878
                     /gene="msrC"
                     /locus_tag="KJL99_002995"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HBD4437642.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFARFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6975..7673
                     /gene="proQ"
                     /locus_tag="KJL99_002996"
     CDS             6975..7673
                     /gene="proQ"
                     /locus_tag="KJL99_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HBD4437643.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKASQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            7693..9741
                     /gene="prc"
                     /locus_tag="KJL99_002997"
     CDS             7693..9741
                     /gene="prc"
                     /locus_tag="KJL99_002997"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HBD4437644.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNAQWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLSKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            9933..10814
                     /gene="htpX"
                     /locus_tag="KJL99_002998"
     CDS             9933..10814
                     /gene="htpX"
                     /locus_tag="KJL99_002998"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HBD4437645.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(10860..12233)
                     /locus_tag="KJL99_002999"
     CDS             complement(10860..12233)
                     /locus_tag="KJL99_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD4437646.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMATGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRARA"
     gene            12410..13201
                     /gene="kdgR"
                     /locus_tag="KJL99_003000"
     CDS             12410..13201
                     /gene="kdgR"
                     /locus_tag="KJL99_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HBD4437647.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLQCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(13344..13583)
                     /locus_tag="KJL99_003001"
     CDS             complement(13344..13583)
                     /locus_tag="KJL99_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4437648.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLEITDCPLLRKSNIVVDNG"
     gene            13742..13885
                     /gene="mgrB"
                     /locus_tag="KJL99_003002"
     CDS             13742..13885
                     /gene="mgrB"
                     /locus_tag="KJL99_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HBD4437649.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            13960..14247
                     /locus_tag="KJL99_003003"
     CDS             13960..14247
                     /locus_tag="KJL99_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HBD4437650.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            14917..15060
                     /locus_tag="KJL99_003004"
     CDS             14917..15060
                     /locus_tag="KJL99_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HBD4437651.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            15073..15282
                     /gene="cspE"
                     /locus_tag="KJL99_003005"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             15073..15282
                     /gene="cspE"
                     /locus_tag="KJL99_003005"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HBD4437652.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            15448..16257
                     /gene="rlmA"
                     /locus_tag="KJL99_003006"
     CDS             15448..16257
                     /gene="rlmA"
                     /locus_tag="KJL99_003006"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HBD4437653.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(16254..16820)
                     /gene="mntP"
                     /locus_tag="KJL99_003007"
     CDS             complement(16254..16820)
                     /gene="mntP"
                     /locus_tag="KJL99_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HBD4437654.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGAPLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(17283..>17548)
                     /locus_tag="KJL99_003008"
     CDS             complement(17283..>17548)
                     /locus_tag="KJL99_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBD4437655.1"
                     /translation="ATLERLLSLLSAFEVVVWMTDGWPLYESRLKEKLHVISKRYTQR
                     IERHNLNLRQHLARLGRMSLSFSKSVELHDKVIGHYLNIKHYQ"
BASE COUNT         4632 a         4240 c         4419 g         4257 t
ORIGIN      
        1 atgaatggca aaaggacgtt gatttgcatc aggtcttgtg gagccgctcg cgattaggtg
       61 aacgcccaaa agggcaggga attacaggtg ctgatcattt ctggtttggt catacaccgt
      121 tgcgacatcg cgtggatatt ggcaacctgc attatattga taccggtgct gtctttgggg
      181 gctaactgac tcttgtgcaa ctgcaataat taaaaatcac catactcctg tgctggttgc
      241 cagaaaccat ctataaaatc ctcaatcgga aaacaactgc catggcggat ccgttgatcg
      301 ctcatagaat aaagacactg ctgttccgtg ttgtagacat ccacaacaat atcttcacaa
      361 ccgccatcca ggtagcaaac aaaaagtacc agcgcgaaca tttcatcccc gaagtgtggt
      421 gccgtaccgt taagtttagg agagatttta caacggggga ataaccagga caaataaccc
      481 gccatgccga cgggttcttt ttggatcagg caagacgcat aatccaggca tcagattgtt
      541 gatcgtaacg ggtacggtaa aggacagagt gttcgccatt tagcagggca gggatactgg
      601 tttcagcatc ccaggcatcc agttgcatac ataaatcggg gtcagattta caaggaatgg
      661 ttaacgttcg atcacccggc gattcaccgt gtaattcgcc gtcgtcgatt tcaaaagcgc
      721 ctatgcgcac actggttttc atcttcgtgc tctccattta gctgctgatt gtagtacgac
      781 cagtccgatc gcacaatttt tcagcctagt tgaggccggc gaatgtgcca gtcaaaaagt
      841 gcgctttttt tgtgcgctgt catgcgttac tggtaaaaag tttcccgtcc cgcaccagtt
      901 cacgcgggta gctgtttttc agtcgcgaac caatccgttt ggctaaacca atcggctgat
      961 gctggaaagt aaccaacacg tcatccgcca ctggcgcggc ttgcgggtaa acatcgcgcc
     1021 cgcgatacca ctcctacgct tcctgcggtg tcagttcaaa agcgtttaca ttgtcggggg
     1081 aggcatgggc aataaccgct tcatgctgcc agcgataacc tttgttatgc gtttcggcaa
     1141 gtttaatccc taagcgagaa aatcggactt taccgatcag ggcttcaatg cctaccggga
     1201 acaaccacag ttctttgtcg cgttgccaga gtcgcaggtt tccatcccag tttaagccaa
     1261 cacttgcagc cgcctgacga atttgtccgg cttcgcgatc tttcaccggg ctgaacggga
     1321 aattgcccac tttgtatttt ggggcgggta aggcggggat cgcctgagtt ttacgcagac
     1381 gagcaacgaa gaagccttcg cagtcgtaaa tttgtgggaa aacatgcaaa aaaccttctt
     1441 cggtcagcgc cttgtttgca ccagggaaga gatcgccaag tggtaaaaac tctacttcgt
     1501 cggggtaagt ctctttcagc caccggcaaa cggcttcgtt ttcttcccgg tttaaggtat
     1561 aggtcgagta aaccagcgta ccgccaggac gtaatgcatg aaaggcgctg tcaatcagtt
     1621 cccgttgggt agctgcgatt tcctgattgc tttcgggtga ccagtttttt agcgcatcgg
     1681 gatctttacg caccacgcct tcgccggagc agggagcgtc cagcaaaatg gcatcaaaca
     1741 tttctggcac tgccgcacca aacacgcggc caccaaaatg tgtgagcgca acattactga
     1801 tgccacagcg gctgatattg gcatgtaaca cttttaccca actggcggaa aactcattgg
     1861 caaggattgc cccttcgtta ttcatccgcg cggcaatttg cgtcgttttt gagcctggcg
     1921 cagcagcgac atccatcacc cgctgtggtg cattaccgtc agcaaacaag gcggcgacgg
     1981 gcaacattga actggcttcc tgaatataaa acaggccact taaatgctcg gtggtactac
     2041 ccaatggcaa tgcatcttca ttgtcgcgtt caatccagaa accttcttca caccacggaa
     2101 ttggcgtaag cgtccagcca taaggagcgg ttaattgcag gaaatcagca acggagattt
     2161 tcagcgtatt aacgcgaatg ctgcggcgca acgggcgctg acaggcggca agaaaatcat
     2221 caaatgagag cgtcgaaggc atagcttcgc gcatttgtgt cagaaacgcg tccgggaaat
     2281 aaacggtgtg ttgggccacg agcatgtacc actggcaaaa aaacaggcgc gcagtttagc
     2341 ataagcgctc cggcgcaggc acgccggagc agtgggcatt atttgggaag cgcagttccc
     2401 cattcacgcc actctttcgg ttcactttcc tgcaacagga agtgtttgcc ttcttgggct
     2461 ttcggtgcaa gtggcgtacc cggaggcgtg gcgaaggcga tgccgccacg gataaattgg
     2521 ttaaaggtgc cggttttcac tacgccgccc gtcagaccaa agtccagact gtaacccgat
     2581 gccaaccaga agacggaatt gttacgcacc aggtgttgat agcgtttact gatgcgcatc
     2641 gcaatcatca cgcgatctga caatgtcccc agcgtcattc cggtaaccgt acctacttcc
     2701 agaccacgga acagcacagg cgtaccgatg cctaacgaac cggcttccgg cgcttcaaca
     2761 ataatgctta aaccatctag gtaacgcgaa tcagtaatgg tggcctcttc taattcaaag
     2821 tcgcgacgag gattgccacg gcctggttcg acgttgatat acggctggag gatagtatct
     2881 agatgctcaa cgccagctgc cgagatttgc ggtgtgacca ctgagaagcg cgtaccaccg
     2941 cgagcaaagg tctggacata ttccggatag agcaccgcct ttgcctgtac ttcattgcgt
     3001 gcggtaatca gatccagcgt ctggatttgc ccgatatcaa taccgagata gcgaattggc
     3061 atcccgaccg ccagttttcc ggcatcgaaa gcgtgaagcg taatctgcct gccaaccgca
     3121 cgggccgctg tttcggaagc atacagaatt cgcttgtcgc ctttacgctg actggcgctg
     3181 gcaccactga ggttatcgaa gctaatggcc ccctttaatg ctctggagag cggggatgcc
     3241 tgtacggtca ggccactacc attcagctga actttcgccc cgccttctgc ccagaacacg
     3301 ctgttgctgg tcaaaaggtt gcgatattcc ggtttaatgt gcagatcgat atcgaacgcg
     3361 ttagctcgcg gacgcacggt aataacttca ccaacttcaa atttacggta cagcactacc
     3421 gatcctgcct gcacatccgg cagcgtctct gcactcaaac tcatggttgt ggtgggtaaa
     3481 tcgctaaggc tgttctccag cgctttttcc agattggcat acagtggata gctggctttc
     3541 atctcgcctt tatcgcccgg cagaatacgt atcccgccgt taatccattc tgaggcgctg
     3601 gcaccgagaa actcaacgcc atccagcccc accttcacgt cgacacggct gttgacgaca
     3661 aatttgctat cgccttttac cagttctcga tgctgaggct cgatggcgac ggtaaaggtg
     3721 acgcctttgc tggtgagttt acgatcgata acctggccta cctgcacgcc gtgaagaatg
     3781 agcggctgac ccgcatcaat accgtaactt tccggtgctg tcagggtcag tgtcagaacg
     3841 tccggctctt gcagcagggc tttttcgccc ggcacaacaa cgaactcttt gcgtggctcg
     3901 ccatcgccgg gcaccaactc aaaggttttt ccggtcagca gggcgctgag attagcatcg
     3961 ctaagggata atttcgggtt gcgtaattcg atgcgggtat tttcacgaag cagggatcaa
     4021 cggtcatttc cccagtgact ttaccaccag gatttaaatc cagtttagtc agctgtccga
     4081 cttccagccc ctgatacatt aacggcgtcg agtcggcggt taatcctgct ccactcggca
     4141 gttccagttt tattattact ccacgctggc tgtgggccag atcttcatac agaccaaagg
     4201 tatcttccgc ctcggcaggt ttcgactctt caggtgaatc gaaggcaatc gcaccgttaa
     4261 ccagtgccgc cagactttcc agtttcacct tcgcgccact gatactgacg ttggcatcaa
     4321 cgccggaaac gtttcagaaa cggctacctt ttttcaccag atcggtaaaa cgccgctcga
     4381 tcaggacatc aatcaccacg ccttgcttgt tgggattgat ggcatagtcg tagacttttc
     4441 ccaccgggat cttgcggaaa tagaccaatg aaccgctgtt cagcgaaccg agagcggggg
     4501 cttgcaggtg gatcatcaga tcgccattgt ccagccgata tttcggttgg gtatcgagcg
     4561 cgacaaagtg atcctgctct ttacctttac ccggcatcat gccgatatag ttcccaccga
     4621 cgagggcgtc cagcccggag acacctgcca acgatgcttt tggcgtcatc agccagaact
     4681 gagtctcttc gcgcagcgca tctttcatat cggacttgat gctgaccttg acttcaatct
     4741 tacgaagatc gtcgctgagg ctgatatcct gcactgttcc gacttcaacg ccctgataac
     4801 gaacaggcgt acggcccgga acaataccat ccgccgacat aaagtcgatg gtgacggtat
     4861 taccccggtc ctgataactg tcccaaatca gccaaccggc aatcattagc gcgatgaaag
     4921 gcagcagcca gaaaggtgag atacggcgtt tatttttaat ctgcgcttca gtcgtcgaag
     4981 cgggcgtttc ctgactcatg tgcatcccaa agtaagcggc tgtccagcca ttccacagca
     5041 agaatagtca aaattacggc agcgccaaaa taaaacgcag ccggtcccat agtaaaagcg
     5101 aggatctgat cgcgattaat cagcgacatg gttaaagata tgacaaacag gtcgagtatc
     5161 gaccagcgtc caatccaggt caccatccgc agtaacagaa tgcgtgtgcg taaaccttgc
     5221 tggcatttaa aatgaatgct caacagtaaa gtgaacatga cgatcacttt agtaaacggt
     5281 accagaatac tggcgataaa cacgattccc gcaaccgcaa tgttgctact tgccagcgac
     5341 ataattccgg aaagaatcgt atcttcctgc cggccaccat tcagataaat gatagaaata
     5401 ggcaataggt tggctggtaa caacaaaacg attgacgcta acagcgccgc ccagcatttt
     5461 tgcaaactat gacgacgacg caggcgtagc gggatatggc aacgcgggca gagaccacgc
     5521 tgatcaggat agccggtaaa atggcaccca agacagacac gaagtttctc gtccctacgc
     5581 gtagcggggc gctgcggata atatcgctcc cacagctcct caacattaag atgtgacaac
     5641 gtcaccgtcg ttaaaatcac caacgccaca aaagagaaca agccgacacc cgcctggata
     5701 tgggcataat cctgtacctt tatagaagca acgccaatgc cgaccaggta gatatccagc
     5761 attacccact ctttaagtcg ctcaagcatc agcagtaccg gacgtaaatt cattcccagt
     5821 cggttaccaa accataaata tgctatggag gacaccagaa tgaggggggc accaataacg
     5881 cagaaaaaga ccattgatcc cgttatcgca tcgccctgtt tggtcatttg ccagatgcct
     5941 tgcatgacgt tggcgtcaat acgaatgcct aacagccaga tatgcaacag cggttcgccc
     6001 caggcaaacg gcataagcaa cagcatggtg aaggccattg ccgccaggcg cgttagcgac
     6061 cagtcgcgcc cgtcgcgaat ttttgcctga cagcgcggac aataggcact ttgatgagaa
     6121 tttatctcgg gcaggctaaa cagcatgtca cattgcgggc aacgtcggta atcaccacgc
     6181 ggcagttcct cgccgattgc cctcactgtt atcttttttg tcggcgtaat ttgtggtgtg
     6241 ttaagagcca tgacgtgcca aatgtaataa gaattaacat taatcttaac ccatgatggg
     6301 aatgatcttg agcattaacg catttaatgc ttattttaat aggctggcag ttccaggtaa
     6361 gacaaccaag tgattaagta atgaacaaaa cagaatttta cgcggattta aatcgcgact
     6421 ttaacgcgct gatggcggga gaaaccagtt ttctggcaac gcttgcgaac accagtgcgt
     6481 tgttatacga gcgtctcact gacgttaact gggcaggttt ttatttgctt gaggacgata
     6541 cactggtact cggaccattt cagggcaaaa ttgcctgtgt ccggataccc gtcgggcgcg
     6601 gggtgtgcgg cacagcggtt gcccgcaatc aagtgcagcg tatcgaggat gttcacgcgt
     6661 ttgacgggca tattgcctgt gatgcggcga gtaattctga aattgttctg ccgctggtgg
     6721 tgaaaaatca gattattggt gttctcgaca tcgatagtac tgtcttcgct cgctttacag
     6781 acgaggacga gcaaggctta cgtcagcttg tggcacagct tgaaaaagtg cttgcaacga
     6841 cggattacaa aaaattcttt gcgagcgtcg caggataatc aacggataac gtagcattta
     6901 ctggtagcgt cattataatg acgcctgttc atgcctgcgc ttgttggcta cgtccgttgt
     6961 aatcaggaaa tttcatggaa aatcaaccta agttgaatag cagtaaagaa gtaatcgcgt
     7021 ttctggccga acgttttccc cactgtttca gtgcggaagg tgaagcgcgt ccgctgaaaa
     7081 ttggtatttt tcaggatttg gtcgatcgtg ttgccgggga aatgaacctg agcaaaacgc
     7141 aattacgatc cgctttacgt ctctacactt cgagctggcg ttatctttac ggtgttaaac
     7201 ccggcgcaac gcgtgtcgat cttgacggca acccatgcgg tgagctggac gagcaacatg
     7261 tagagcatgc tcgcaagcag cttgaagaag cgaaagcgcg tgttcaggca cagcgtgctg
     7321 aacagcaagc gaaaaaacgc gaagctgccg cagctgctgg tgagaaagaa gacgcaccgc
     7381 gccgcgaacg caagccacgt ccgaccacgc cacgccgcaa agaaggcgct gaacgtaaac
     7441 ctcgtgcgca aaagccggta gagaaagcgc caaaaacagt aaaagcacct cgcgaagaac
     7501 agcacacccc ggtttctgac atttcagctc tgactgtcgg acaagccctg aaggtgaaag
     7561 cgagtcaaaa cgcgatggat gccaccgtat tagaaatcac caaagacggc gtccgcgtcc
     7621 agctgaattc gggtatgtct ttgattgtgc gcgcagaaca cctggtgttc tgaaacggag
     7681 gccgggccag gcatgaacat gttttttagg cttaccgcgt tagctggcct gcttgcaata
     7741 gcaggccaga ccttcgctgt agaagatatc acgcgtgctg atcaaattcc ggtattaaag
     7801 gaagagacgc agcatgcgac ggtaagtgag cgcgtaacgt cgcgcttcac ccgttctcat
     7861 tatcgccagt tcgacctcga tcaggcattt tcggccaaaa tctttgaccg ctacctgaat
     7921 ctgctcgatt acagccacaa tgtgctgctg gcaagcgatg ttgaacagtt cgcgaaaaag
     7981 aaaaccgagt taggcgatga actgcgttca ggcaaactcg acgttttcta cgatctctac
     8041 aatctggcgc aaaagcgccg ttttgaacgt taccagtacg ctttgtcggt actggaaaag
     8101 ccgatggatt tcaccggcaa cgacacttat aaccttgacc gcagcaaagc gccctggccg
     8161 aaaaacgagg ctgagttgaa cgcgcagtgg gacagtaaag tcaaattcga cgagttaagc
     8221 ctgaagctga caggaaaaac ggataaagaa attcgtgaaa ccctgacacg ccgctacaaa
     8281 tttgccattc gtcgtctggc gcaaaccaac agcgaagatg ttttctcgct ggcaatgacg
     8341 gcgtttgcgc gtgaaatcga tccgcatacc aactatcttt ccccgcgtaa taccgaacag
     8401 ttcaacactg aaatgagttt gtcactggaa ggtattggcg cagtgctgca aatggatgat
     8461 gactacaccg ttatcaattc gatggtggca ggtggtccgg cagcgaagag taaagctatc
     8521 agcgttggtg acaaaattgt cggtgttggt caaacaggca agccgatggt tgacgtgatt
     8581 ggctggcgtc ttgatgatgt ggttgcctta attaaagggc cgaagggcag taaagttcgt
     8641 ctggaaattt tacctgctgg taaagggacc aagacccgta ctgtaacgtt gacccgtgaa
     8701 cgtattcgtc tcgaagaccg cgcggttaaa atgtcggtga agaccgtcgg taaagagaaa
     8761 gtcggcgtgc tggatattcc gggcttctat gtgggtttga cagacgatgt caaagttcaa
     8821 ctgcagaaac tggaaaaaca gaatgtcagc agtgtgatca tcgacctgcg tagcaatggc
     8881 ggtggggcgt tgaccgaagc ggtatcgctc tcaggtctgt ttattcctgc gggtcccatt
     8941 gttcaggtcc gcgataacaa cggcaaggtt cgtgaagaca gcgataccga cgggcaggtg
     9001 ttctataaag gcccgctggt ggtactggtt gaccgtttca gtgcttcggc ttcagaaatc
     9061 tttgccgcgg caatgcagga ttacggtcgt gcgctggttg tgggtgaacc gacgtttggt
     9121 aaaggcaccg ttcagcaata ccgttcattg aaccgtattt acgatcagat gttacgtcct
     9181 gaatggccag cgctgggttc tgtgcagtac acgatccaga aattctatcg cgttaacggc
     9241 ggcagtacgc aacgtaaagg cgtaacacca gacatcatca tgccgacggg taatgaagaa
     9301 acggaaacgg gtgagaaatt cgaagataac gcgctgccgt gggatagcat cgatgccgcg
     9361 acttatgtga aatcaggaga tttaactgcc tttgaaccgg agctgctgaa ggaacataat
     9421 gcgcgtatcg cgaaagatcc tgagttccag aacatcatga aggatatcgc gcgcttcaac
     9481 gctatgaagg acaagcgcaa tatcgtttct ctgaactacg ctgtgcgtga gaaagagaat
     9541 aatgaagatg atgcgacgcg tctggcgcgt ttgaacgaac gctttaaacg agaaggtaaa
     9601 ccggagttga agaagctgga tgatctatcg aaagattacc aggagccgga tccttatctg
     9661 gatgagacgg tgaatatcgc actcgatctg gcgaagcttg aaaaagccag acccgcggaa
     9721 caacccgctc ccgtcaagta atatcaatca ggcacaagaa attgtgcctg attttttaac
     9781 agcggcaaga tgccgtaaat cagatgctac aaaatgtaaa gttgtgtctt tctggtgact
     9841 tacgcactat ccagacttga aaatagtcgc gtaacccata cgatgtgggt atcgcatatt
     9901 gcgttttgtt aaactgaggt aaaaagaaaa ttatgatgcg aatcgcgctc ttcctgctaa
     9961 cgaacctggc cgtaatggtc gttttcgggc tggtactgag cctgacaggg atacagtcga
    10021 gcagcgttca ggggctgatg atcatggcct tgctgttcgg ttttggtggt tccttcgttt
    10081 cgcttctgat gtccaaatgg atggcattac gatctgttgg cggggaagtg atcgagcaac
    10141 cgcgtaacga aagggaacgt tggctggtca atactgtagc aacccaggct cgtcaggcgg
    10201 ggatcgctat gccgcaagtg gctatctacc atgcgccgga catcaacgct tttgcaaccg
    10261 gtgcgcgccg tgatgcctct ctggttgctg tcagcaccgg tttgctgcag aacatgagcc
    10321 cggatgaagc cgaggcggta attgctcacg aaatcagcca catcgccaat ggtgatatgg
    10381 tcaccatgac gctgattcag ggcgtggtga acaccttcgt tatctttatt tcccgtattc
    10441 tggcgcagct tgccgcgggt tttatgggcg gaaatcgtga tgaaggtgaa gagagcaacg
    10501 gcaacccgct gatctacttt gcggttgcaa cggttctgga actggtgttt ggtattctgg
    10561 cgagcattat caccatgtgg ttctcgcgtc atcgtgaatt ccatgcagat gccggttcgg
    10621 caaaactggt tggtcgcgag aaaatgattg ccgcgctgca gcgcctgaaa accagctatg
    10681 aaccgcaaga agcaaccagc atgatggctt tctgcattaa cggtaagtcg aaatcgctca
    10741 gtgagttgtt catgactcac ccgccgctgg ataaacgtat tgaagctctg cgtacgggtg
    10801 aatacctgaa gtaatatgaa gtaaatacta aaaagcgcgt cctgatcgac gcgctttttt
    10861 tatgccctgg ctcgtggctg agtgatacgt aaaccactga cacaggcagc aatcactgcc
    10921 agaatagccg cagccatcag cgagacgtgc gtaccattat caccaaactg atttagcatc
    10981 agcgccacca gcgccgcgcc actactctga cccagtagac gagccgttcc taacatgcca
    11041 ctggccccac cgctacgttc gcgcggagcg gaggtaataa tggtgtggtt attgggtgac
    11101 tggaataagc caaatccagc gccacataag atcatcggcc agataatatt gatatccgca
    11161 ggtgacgcgg gcagcagaac cagggaaaaa agccccgtag ccatgatgaa caaccctaaa
    11221 gcacccagca atcctgcatg tacgcgttca atcaaatagc ctgccagagg agccatcacc
    11281 atcgttgcta acggccacgg tgtcagaagt aaacctgttt cgacttcact acgcccgagc
    11341 acggtttgca ggtaaaaggg cagggaaacc attgccagca tttgtgcgca gaaagagcaa
    11401 acagatgtgc aaatagaaag tgaaaacagc gggatacgca gtaaatccac cggtagcagc
    11461 ggtacgggaa gagaaagctg gcggcgaata aagaaaatac caacaacaac cattaccacc
    11521 agttccgcag caattaacgt cagcgattgc ccctgagcga aaccactcaa cgcagtgata
    11581 agcaggccga aggttaacgc gttcatcacg gcgctgggca ggtcgaaacg gggtttactg
    11641 gcgcgagaac cattgggtgg cagaaaacgc atcgccagaa gcagggcgat aatacctaac
    11701 ggtacgttga ttaaaaataa ccatttccag gatgcgatgg agaggattgc tgcagcaatt
    11761 gtcggcccgg cagcagaaga gacggcaaca ataaacgagt ttatgcccat ccctctcccc
    11821 agaaaacgtt gtggatagat caggcggata agtgcggtat taacgctcat caacgctgca
    11881 ccgccgaaac cttgtatgac acgcgcaagg gtgagcattt gcagcgaatc agaaagggcg
    11941 cagaacagtg aagacaacag aaaaacgacc agaccgcatt tataaatacg tcgatagcca
    12001 aacatatcgc ccagaaacga aaacgagagc agggagatga caatggcgat ttgataggcg
    12061 ttcactaccc agatggaact ggctggtgtg gcatgaaggt ccgtggcaat tgttggcagg
    12121 gcgacgttgg cgattgcgcc gtcaaggaca gccatcgaaa taccaatcac aatggttaat
    12181 atcgcaccgt atcgctgggg caatggcagg ccgtcggcct gaacttttgg cataggaatt
    12241 ttatatcttt ggtgaataat caacaggggt atgattctag cactgatatt cacggtggat
    12301 gtcgcagaat tgtaactaac cggcaaaaga tggattgcga ccaccctggt gcgaaattat
    12361 aataaaaaac agttctgatt tttataaaac actcgcaatg aggtgataaa tggctaacgc
    12421 agatctggat aaacagcctg attctgtatc ttccgtgcta aaagtttttg gcattttgca
    12481 ggcgctgggt gaagagcgcg aaatagggat aaccgagctg tcgcagcgcg tcatgatgtc
    12541 aaaaagcacc gtttatcgct ttttacagac catgaaaacc ttaggttatg tggcgcagga
    12601 aggggagtcg gagaaatatt ccctgaccct gaaattgttt gaactgggcg ctcgcgcgtt
    12661 acaaaacgtc gatttaattc gtagcgcaga tatccagatg cgtgagatct cccgcctgac
    12721 caaagaaact atccacctcg gcgcactgga cgaagacagt attgtttaca ttcacaaaat
    12781 tgactctatg tacaatttgc gcatgtattc acggattggg cgtcgtaatc cgctgtacag
    12841 caccgcgatt ggtaaggtac tgctggcatg gcgcgatcgc gatgaagtga agcaaattct
    12901 tgagggcgtg gagtataaac gcagtaccga gcggaccatc accagtacag aagcgttatt
    12961 acccgttctg gaccaggtgc gcgagcaggg gtatggagaa gataacgaag agcaggaaga
    13021 agggctgcaa tgcattgcgg taccggtatt tgatcgcttt ggcgtggtca ttgccggttt
    13081 gagcatctcc ttcccgacgt tgcgtttctc tgaagagcgt ttacaggaat atgtcgcaat
    13141 gttgcatacc gcagcgcgca aaatttctgc ccaaatgggt tatcacgact atccgttctg
    13201 atgagagtaa gaacctgtcg gaatatcaaa cagacaggtt ctttatttag catgagaaaa
    13261 ataaagttga aggtgcgtta tattaaacgc gcttgctata agaatatttt actcatgagt
    13321 gagaatctgg ttatttatta cccttaacca ttatcgacca cgatattgct tttacgtaac
    13381 agtgggcaat ctgttatctc caagaaacca cttttagtgt gcaagtattg taccgtgctg
    13441 gtgcctctgg cagtcagata ggtacattgc aaacctaatc ctgcggcatt ctctttgctt
    13501 ccaatcaaaa cgccatatcc gctgagtaat aatcctatcc ataccagtgc tatcagcata
    13561 actgtgcgaa tgatgaatcg cattacaatc tcttctcttt ttatgttcgc ttaatcgtag
    13621 cggcaatatg cgctgaagca agctactcat tccgaaaaag cacgaatatc gacatagtta
    13681 ggcgctgttt aactaacgca tgctagttta atgacataag gtaggtgaag cggagattgg
    13741 agtgaaaaag tttcgatggg tcgttctggt tgtcgtggtg ttggcttgct tgctgctttg
    13801 ggcgcaggta ttcaacatga tgtgcgatca ggatgtacaa tttttcagcg gaatttgtgc
    13861 cattaaccag tttatcccgt ggtgatatca ttttttcagc actagatttt cttcctctcc
    13921 agcagtggta gaatagcgcc tctactcaga ggtggtgaaa tgaacgaagt tgtaaattca
    13981 ggcgtgatga acattgcgtc tttggttgta tcggtggtgg ttcttcttat cgggctcatc
    14041 ttgtggtttt ttatcaatcg tgccagttca cggactaacg aacagattga actgcttgag
    14101 gcgttgctgg atcagcaaaa acgtcaaaat gcactgttac gtcgtttgtg cgaagcaaac
    14161 gaaccagaga aagcagataa aaagaccatt gagagtcaaa aatcggttga tgacgaagat
    14221 attattcgcc tggtcgccga acgataatcc ttcctcaatc ttgagtaacc ccgtcgttga
    14281 agggttactc aattcatcct gattatacgc actgtcatgg tgcaactgct tcaaattaaa
    14341 actctggtct gacttcaaat aattatcata ttatttattt gcgtaacggg atatcagaaa
    14401 aagatatttt cctcattaat ttattaggat gtttacatca gatttgtgat taagcgtggt
    14461 attatttatt agcgaaacgt ttctctcttg atttttttgc tcactcatca atttttctta
    14521 ttttaaattt acaatccctt tggggattga cttctcttta gggtaattga tagccgttaa
    14581 ctgactgttt tatgagaaaa agtgatataa ctttttattc attgcatagc agaaaatgtg
    14641 atattgcacg cactatgtaa taacttctcc ctctggcctg gaacaactga acttattgaa
    14701 ctatgttaga aactacgcca gtttaagtat ctgcctgaac tggcaaggtt aagcacaatg
    14761 atatatcggc gcgtattccg ttgcataagt gtgcaaaaaa agtggaagac gtatcgagat
    14821 ttgtgcgtct gatcgagata tgtttaaaaa tggcttgcca taattaacgt tgtatgtgat
    14881 aaaagatttc gggttaaacg aggtacagtt ctgtttatgt gtggcatttt cagtaaagaa
    14941 gtcctgagta aacacgttga cgttgaatac cgcttctctg ccgagcctta tattggtgcc
    15001 tcatgcagta atgtgtcagt tttatctatg ttatgcctgc gggcgaagaa aacaatctaa
    15061 ggaatttttc aaatggcaaa gattaaaggt caggttaagt ggttcaacga gtctaaaggt
    15121 tttggcttca ttactccggc tgatggcagc aaagatgtgt tcgtacactt ctccgctatc
    15181 cagggtaatg gcttcaaaac tctggctgaa ggtcagaacg ttgagttcga aattcaggac
    15241 ggccagaaag gtccggcagc tgttaacgta acagctatct gatcgaatcc actgatctga
    15301 agtgtgaata cgcttcaatc tcgctataaa gcctcgtcga atgcgaggct ttttactatg
    15361 ctttatcttc gctcctggcg ttcggatatt tgcccgccgc gtgattcgcg ttacacttgc
    15421 ggcctttagt atcctgccgg agttgtcatg tcgttttcct gtccactttg ccatcagcct
    15481 ctttcgcgtg aaaaaaacag ctatatctgt ccccaacgac atcagtttga tatggcgaaa
    15541 gaagggtatg tcaatctgct gcccgttcag cataaacggt ctcgtgatcc gggcgacagc
    15601 gcggaaatga tgcaagcacg ccgcgcattc ttagatgccg gacattatca gccgctgcgt
    15661 gatgcaattg tcgcccaact gcgggaacgg cttgatgaaa aggccacggc ggtgctggat
    15721 attggctgtg gtgaagggta ttacacacac gcatttgccg atgcgttgcc cgaaatcacc
    15781 acgtttggcc tggatgtctc gaaggtagcg ataaaagcgg cggcgaaacg ctatccgcag
    15841 gtcactcttt gtgtcgcttc cagccaccgt ttgccgtttt ccgataccag tatggacgcc
    15901 ataatacgta tttacgcgcc gtgtaaagca gaagaattag cacgagtagt gaagcccggc
    15961 ggctgggtca ttactgccac gccgggaccg cgacatttga tggagctgaa ggggctgatt
    16021 tacaatgaag tacatcttca tgcacctcat gcagaacaac tggaaggttt tacattacag
    16081 cagagtgcgg agttgtgtta tccgatgcgt cttcgcggtg atgaagccgt cgcattattg
    16141 cagatgacgc cgtttgcctg gcgtgcgaag ccagaagtct ggcaaacact ggcagcaaaa
    16201 gaagtgttcg actgccagac ggactttaat attcacctct ggcagcgttc ttattaaccg
    16261 tggaagtgcg tccagaggat ctggacgccg atgccgatca gcaccagccc gccgagaatt
    16321 tccgcttttt tcccaataat tgagccgata aagcgaccaa ccatcatccc taatgtcgac
    16381 ataatcaagg ttgcacaacc aatggccaat gcggtcgcga taatgttgac ctgaaggaaa
    16441 gcaagaccaa cacccacagc catggcatcc aggctggtgg caatcgcggt ggttaccagt
    16501 agccagaaac cgtgccgacg gcgcggctct tcatcttcat catctgcgcc acgaaaaccc
    16561 tcaataatca ttcgcccgcc gaggaatatc agcagcacaa acgcaatcca gtgattccat
    16621 tcaaggacaa accggctggc taacatgccc attccccagc cgatcagcgg cgtcagggtt
    16681 tcgacggcac caaaaataag gccggttcgc aatgcttcag aaaatttcgg tttatggagg
    16741 ggggcacctt taccgattga tgcagcaaat gcatccatcg acataccaaa cgcaagaaga
    16801 acagtagcag tgatattcat gacaacgtcc tgaccggggt atccataaaa cacatcatcg
    16861 cccccagcaa acatgcatac atgaacaaca agccgcgatg ggttttcgcg ttcatgcagg
    16921 catagcgatt gatgtgcata tggtctcgcc tgactgaaag tcttcagtcc gtacgtgcca
    16981 cgttttgcaa cgagtatgtt gacacgtaca tttccggaat ctggaaatcg gctactcccc
    17041 aaatgagggc gcaaccttaa catattttga gaatataaaa caactgttgg aatatattat
    17101 ttcattgctt tattgataac gattttcatt tatgtttata tatcaatgaa acgttaaaat
    17161 aaataacaaa taagggaaga gataatatag cctaagctat gtctgattat taaaaataca
    17221 ctaaacatag ctttggctaa attcataagt tattgagttg atacgagggt aatgactcca
    17281 acttactgat agtgttttat gttcagataa tgcccgatga ccttgtcatg cagctccacc
    17341 gattttgaga acgacagtga catccgtccc agccttgcca gatgttgtct cagattcaga
    17401 ttatgtcgct caatgcgctg agtgtaacgc ttgctgataa cgtgcagctt ttccttcagg
    17461 cgtgattcat acagcggcca gccatccgtc atccatacca cgacctcaaa ggccgacagc
    17521 aggctcagaa gacgctccag tgtggcca
//
LOCUS       DAEBXN010000089        17091 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.089, whole genome shotgun sequence.
ACCESSION   DAEBXN010000089 DAEBXN010000000
VERSION     DAEBXN010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17091
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.089"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..510
                     /locus_tag="KJL99_003009"
     CDS             <1..510
                     /locus_tag="KJL99_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HBD4437656.1"
                     /translation="PFREKPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDG
                     FTQPIVVTHTDKNAMEIVDGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATI
                     RHNRARGRHQITAMSEIVRELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADR
                     QYSRAWTVK"
     gene            complement(511..1671)
                     /gene="ybdL"
                     /locus_tag="KJL99_003010"
     CDS             complement(511..1671)
                     /gene="ybdL"
                     /locus_tag="KJL99_003010"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HBD4437657.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            1780..2868
                     /gene="hcxA"
                     /locus_tag="KJL99_003011"
     CDS             1780..2868
                     /gene="hcxA"
                     /locus_tag="KJL99_003011"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HBD4437658.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHNFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(2878..3075)
                     /locus_tag="KJL99_003012"
     CDS             complement(2878..3075)
                     /locus_tag="KJL99_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HBD4437659.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(3257..5362)
                     /gene="cstA"
                     /locus_tag="KJL99_003013"
     CDS             complement(3257..5362)
                     /gene="cstA"
                     /locus_tag="KJL99_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HBD4437660.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQSVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTADWLKAFSPDAKVGFLVIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(5543..5956)
                     /gene="entH"
                     /locus_tag="KJL99_003014"
     CDS             complement(5543..5956)
                     /gene="entH"
                     /locus_tag="KJL99_003014"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HBD4437661.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTLLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(5959..6705)
                     /gene="entA"
                     /locus_tag="KJL99_003015"
     CDS             complement(5959..6705)
                     /gene="entA"
                     /locus_tag="KJL99_003015"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HBD4437662.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALTFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAGQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTARIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(6705..7562)
                     /gene="entB"
                     /locus_tag="KJL99_003016"
     CDS             complement(6705..7562)
                     /gene="entB"
                     /locus_tag="KJL99_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HBD4437663.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(7576..9186)
                     /gene="entE"
                     /locus_tag="KJL99_003017"
     CDS             complement(7576..9186)
                     /gene="entE"
                     /locus_tag="KJL99_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HBD4437664.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSAEIC
                     QFTQQTRYLCSIPAAHNYTMSSPGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEDIENL
                     LLSHPAVIYAALVSMENELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(9196..10371)
                     /gene="entC"
                     /locus_tag="KJL99_003018"
                     /pseudo
     CDS             complement(9196..10371)
                     /gene="entC"
                     /locus_tag="KJL99_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
     gene            10663..11619
                     /gene="fepB"
                     /locus_tag="KJL99_003019"
     CDS             10663..11619
                     /gene="fepB"
                     /locus_tag="KJL99_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HBD4437665.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(11623..12855)
                     /gene="entS"
                     /locus_tag="KJL99_003020"
     CDS             complement(11623..12855)
                     /gene="entS"
                     /locus_tag="KJL99_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HBD4437666.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATSALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPELPPPPQPLEHPLKSLLAGFR
                     FLLASPLLGGLLTMASAVLVLYPALADNWQMSAAQIGFLYAAIPLGAAIGALTSGKLA
                     HSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYTMLQTQT
                     PEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGVLLLLVL
                     VELRRFRQTPPQVTASDS"
     gene            12966..13970
                     /gene="fepD"
                     /locus_tag="KJL99_003021"
     CDS             12966..13970
                     /gene="fepD"
                     /locus_tag="KJL99_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HBD4437667.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLSGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            13967..14959
                     /gene="fepG"
                     /locus_tag="KJL99_003022"
     CDS             13967..14959
                     /gene="fepG"
                     /locus_tag="KJL99_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HBD4437668.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAATLLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAAVLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            14956..15771
                     /gene="fepC"
                     /locus_tag="KJL99_003023"
     CDS             14956..15771
                     /gene="fepC"
                     /locus_tag="KJL99_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HBD4437669.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGMPLVVPLGRTAPSTANS"
     gene            complement(15768..16901)
                     /gene="fepE"
                     /locus_tag="KJL99_003024"
                     /pseudo
     CDS             complement(15768..16901)
                     /gene="fepE"
                     /locus_tag="KJL99_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
BASE COUNT         3994 a         4864 c         4443 g         3790 t
ORIGIN      
        1 ccctttcgtg aaaagccagt ggattgcgtt ctgtgggtta aaaacagcca acttatgcct
       61 aatgattaca acccaaacaa cgtggcacca cccgaaaaaa aactgctgca aaaatccatt
      121 gaaatagatg gttttaccca acccattgtg gtcacacata cggataaaaa tgccatggaa
      181 atcgtggatg gttttcaccg ccatgaaatt ggtaaaggct catcatcatt aaaattacgc
      241 ttaaaaggtt atttgccggt gacctgtctg gaaggtacgc gtaatcaacg catcgccgcg
      301 actattcgtc ataatcgcgc tcgtggacgc catcaaatca ccgccatgtc ggaaatcgtc
      361 cgtgaactta gccagttggg atgggatgat aataaaattg gcaaagagct gggaatggac
      421 agcgacgaag tgctgcgact gaagcaaatt aacggcctgc aagagttgtt tgccgaccgt
      481 caatattccc gcgcctggac ggttaaatag ctaaagctgg cgcaggcgtt cagctgctgc
      541 cagcaacgtc gattccttct tggcaaaaca gagacgaatc agtttatgtg ggaagggatc
      601 ggcgcaaaac accgacagcg gaatcgccgc tacgccgtgc tcctgcgtca gccactggca
      661 aaactcaaca tcatccaggg tagaaaccgc gctgtaatcc accagcaaaa agtatgtacc
      721 ttcacacggt aaaatctcca gccggctttc atttaaagca ttcaccagaa tatcgcgctt
      781 ctggcgataa aagtccggta acgcaagata atgctcaggt tctgcacgta gcatatcagc
      841 aagcgccagc tgtgccgggg tattcaccga aaaggtcaga tactgatgta ccttgcgaat
      901 ttcggcgctg atgggcgctg gcgcaacaca ataacccact ttccagccgg tcatatgata
      961 ggtcttgcca aatgaagaaa ccgccactgc ccgctcacgc agctgcggat gcgccagcac
     1021 actggcatgg ccctgttgtg aaaagttgat gtgctcgtag acttcatcgc taatgacaaa
     1081 aatctcgtgc ccggcgatcg cctgccacaa agcggcgaaa tcagcctgct gccagacagt
     1141 tgcactgggg ttatgcggag tgttgaggat caccagtctg gtgcgctcgc ttaataatgc
     1201 ggcaaattcc tgccagtcaa cgcgaaaatg cggtggttgc agtgccatac gtttcactat
     1261 tcccccagaa agcgcgatgg cgggggcgta actgtcatag ctgggatcaa aacaaatcac
     1321 ttcatcgcca ttgcgcacca gtgcggtaat cgccgcgtat aacgcttccg tcgcccctgc
     1381 cgttacggtg atatcgctat cggcatctgg ttgatagcca tacaaacgtt ccgttttctg
     1441 agcaatcgcc tcgcgcaagg cctgcacgcc ggtcatgggc gcgtattggt ttgccccctg
     1501 ggcaacgtgg tacgccagcc gctcctgtaa atagcgcgga ccatcaaaat caggaaagcc
     1561 ttgcgacagg ttaatcgcct ggtgttgctg cgccagcgcg ctcatctggg tgaaaatagt
     1621 ggtgccaagt tgtggaagtt tgctttgtgg aatcagaggg ttatttgtca tttttattgt
     1681 acctgacgaa gttgcgtgtt gaaggcacta taacacgatg ccagtatttg gcaatcaaga
     1741 cgtttagatg tctaaataaa acaataagga caacacaaca tgcctcacaa tcctatccgc
     1801 gtggtcgtcg gcccggctaa ctacttttca catccaggaa gtttcaatca cctgcacaat
     1861 tttttcactg atgaacaact ttctcgcgcg gtgtggatct acggcaaacg cgccattgct
     1921 gcggcgcaaa ccaaacttcc gccagcgttt ggacttccag gggcaaagca tattttgttt
     1981 cgtggtcatt gcagcgaaag cgatgtacag caactggcgg ctgagtccgg tgacgatcgc
     2041 agcgtggtga ttggcgtcgg cggcggcgca ctgctcgaca ccgcgaaagc cctcgcccgc
     2101 cgtctcggtc tgccatttgt tgccgctccg acgatcgccg ccacctgcgc agcctggaca
     2161 ccgctctccg tctggtataa cgatgccgga caggcgctgc attatgagat tttcgacgac
     2221 gccaatttta tggtgctggt ggaaccggag attatcctca atgcaccgca acaatatctg
     2281 ctggcgggga tcggtgacac gctggcgaaa tggtatgaag cggtggtgct ggctccgcaa
     2341 ccagaaacgt tgccgctaac cgtgcggctg gggatcaata atgcgcaggc cattcgcgac
     2401 gtcttgttaa acagtagcga acaggcactt gccgatcagc aaaatcagca gttaacgcaa
     2461 tcattttgcg atgtggtgga tgctattatt gctggtggtg ggatggtcgg tggtctgggc
     2521 gatcgtttta cgcgtgtggc ggcggctcat gccgtgcata acggtctgac tgtcctgccg
     2581 caaaccgaga agtttcttca cggcaccaaa gtcgcttacg gaattctggt gcaaagcgcc
     2641 ttgctgggtc aggatgatgt gctggcgcaa ttaaccgggg cgtatcagcg ttttcatctg
     2701 ccgactacgc tggcggagct ggaggtggat atcaataatc aggcggagat cgacaaagtg
     2761 attgcccaca ccttgcgccc ggtggagtcc attcattacc tgccagtcac gctgacacca
     2821 gatacgttgc gtgcagcgtt cgaaaaagtg gaatcgttta aagcctgacg tgcaaattta
     2881 gcagcagcgc gcgccgcctt ttccaccgta gcgcgcgtcc tgccgctcac ggaaaaactc
     2941 ctcataggtc atcggcgttt gatcgggatg attaacccgc atatgttcga catagttgtc
     3001 gtaatcaggc ataccaatca tcagcttcgc cgcctgacct aaatattttc cggcttttgc
     3061 cagtgaatca aacatcttgg cctccgctaa ccatgttgct gaaagagtgc cggatgcggc
     3121 gtgaacgctt tatccggcct acaaatgcta tcaacgcact cggtcagtcc cctcgcccca
     3181 tcggggagag ggttagggtg agggggaacc ctcggcacta tgccaacatt cgccaacatc
     3241 ccccctcact ctgactttag tgcgcgcctt tcgcctgcgc tacgatctcc tcgacatttt
     3301 ccggcatcgg ctcatacggc gtttctttcg ccgttggctt cggatctttc aatgccgcaa
     3361 gtgccgtctt aatcgagaac agtgccagga ccaccacgac caccataaag aagatggtta
     3421 acccggcatc cagacggttg ttgaacacca gttgcgccag ctgtgactca gtatactgcg
     3481 acggaatatt gccgctgtcg atcattgcct ggaacttgtt agcaatgacc aggaagccga
     3541 ctttcgcatc cgggctaaac gctttcagcc agtcagcggt cagggtacaa atcagcagcc
     3601 aggcagttgg taccagcgcc acccaggcgt aacgttgacg tttcatcttg aacaacacca
     3661 cggcacagag catcagcgcc atccctgcca gcatctggtt ggcaatacca aacagcggcc
     3721 acagagtgtt aataccgccc agcggatcga ccacgctctg atggaggaag tagccccacg
     3781 ccagtacgca cagcgctgtt gccagcaggt tagcaggcag tgaatcggtc cgcttcaggc
     3841 caggagacac cacgcccagc agatcctgca acataaagcg cgcagcacgc gtacccgcat
     3901 ctaccgccgt cagaataaac agagcttcaa acagaatggc gaagtgatac cagaacgcca
     3961 catccatcat accacccagc gcaccgtgca ggatgtaagc catccccacc gccaacgtcg
     4021 gcgcaccgcc cgcacgggaa atgatcgact gttcacccac ttcgctggca atctggttga
     4081 gcgtatctgg tgtaatgcta aagccccagc tactcaccac ctgcgcggca gaagcgacta
     4141 catccgccgt ccctgccgga gccagcaccg ccattgggct gttcatggca aaatacacgc
     4201 ccggatcgat gatacaggca gaaactagcg ccataatcgc cacgaaggat tccatcaaca
     4261 tcccaccgta gccgataaag cacgcctgcc cttcgttcgc cagcatcttc ggcgtggtgc
     4321 cggaagagat cagcgcatgg aagccagaca ccgcaccaca ggcgatggtg ataaacagga
     4381 acgggaacag gttaccggtc cataccgggc cagtgccatc gacaaatttg gtcagcgcag
     4441 gcatggtcag cgtcgggcgc ataatcaaaa tgcctaccgc cagaccgacg atagtcccga
     4501 ttttcaggaa ggtcgagaga tagtcacgcg gggccagcag caaccacacc ggcagcaccg
     4561 ccgccacaaa accgtagccc accagcatcc aggtcagctg cacgccggta aagtcaaagt
     4621 acggtgccca ggtcggactt tctgccaccc agccgccaga gataatggcg aaaatcagga
     4681 acaccaggcc aatcactgac acttcgccaa tgcggcccgg acgcagatag cgcaggtaga
     4741 ttcccataaa aatcgccagc ggaatggtga acgcaacggt atacgttccc cacgggctat
     4801 gagtcagggc tttcaccacg atcatcgcca gtactgcaag gataatgacc atgatcataa
     4861 agcaggctac cagcgcaatc accccggcag ttggccccat ctcttctttg accagctcac
     4921 ccagcgagcg accgtcacgg cgcgtagaaa caaacagcac catgaaatcc tgcaccgcac
     4981 cggcgagaac caccccggcg agcagccaga tcatccccgg caggtagccc atttgcgctg
     5041 ccagcaccgg ccccaccagc ggccctgctc cggcaatggc cgcaaaatgg tgaccgaaca
     5101 gcactttctt gtccgtcggc acatagtcca gcccgtcgtt atggcgcacc gctggcgtca
     5161 tacgcgtcgg gtcaaccgcc agcacatttt tggcgatata cagcccataa aaacggtaag
     5221 cgatcagata gatacagacc gacgccacca caatccacag cgcgttgatc tgttccccac
     5281 gatttaaagc aatgtatccc agagcaaatg ctcccattac agagagcact gtccagacga
     5341 ggtatttccc tgatttgttc atagttgtta tccgtgtgcg tgtccataga gatgttacat
     5401 tttgtttcta taacatcttt gtacgattta acaacacggt aaccatgtaa atcggagttg
     5461 aaaccaatat ttaacttaac aatgttaact cgatcactcc gttaaccgag ttttcctgca
     5521 atctcattta attctgtcgg gctcatccca aaactgccgt acccagccga caagtgcagc
     5581 aacgccgccc ctgttcatcg aaaacgacga tttcccaact ctgattttgc cgcccaagat
     5641 gcagcggctg gcagacgccg cgtaccttgc cttcagacac cgggcgatga tgggtggcgt
     5701 taagttccgt gcccaccaca cactgcccgt cacgggtcat cataaatccg gccatcgatc
     5761 ccagcgtttc agccagcgcc gccgacgcgc cgccatgcag caggccaaac ggctgatgag
     5821 tacgggtatc aaccggcatt tcggcttcca gcacatcatc tcccagaagg gtatacacaa
     5881 ttcccagatg cgccaccatt gtgttatcgc tggtggcgtt cagttcgtcg agcgttaaat
     5941 ggcgtttcca gatcatgctt atgcccccag cgttgagccg ccatcgacca caatatcctg
     6001 tagggtgata tggctggcga ggtcagaggc gaggaacaaa atcgtgttgg cgatctcttg
     6061 tggacgggcg attttcccca gcggaatgcc gagtttaaac tgctcgccaa agccgcgaat
     6121 acgctgttct tcggcgtcat cgctcaccca cagcgtgcgt tgcatatcgg tgtcggtgga
     6181 gccaggcgaa accacattac agcgcacgcc gctacccgcc agttccagcc cgacgctcaa
     6241 cgccaggctt ttcagcgccg ctttcgatgc gccataagca ctcataccaa tacgcgccgt
     6301 gtgtgcggcg tcggacgcca cagtgacaat cgccccgccc cgctgacggc gaaactggtt
     6361 catggtttgc tggaacaggt taaacgcacc gccgacgtta accgcaaaag tctgctgcca
     6421 gtcctcttta ctgagctgat cggtcgcgcc catgcgcaaa attcccgccg cattgatcag
     6481 cacgtccagt cgctccgttt cagctaacag tcgctgacat acctgcgcca cctgcccggc
     6541 gtcggcaaca tccattactt cggtcgcaaa gggatattgc tcctgagtga acgcttgatc
     6601 aaaacctgta actttcgctc ccgcctcaac aaacgtcagc gccgtggcgt agccgatacc
     6661 tttacctgcg ccggttaccc agacattttt accgctgaaa tccattattt cacctcgcgg
     6721 gagagtagct tccaccaggc gtcgatggtc gggtttttcg ccagcatgac aaagtcgata
     6781 tcaccatgca ctttgcgcca gcgcgccgcc agcgccatca tgcgcaccga atccagaccg
     6841 tagtcgatca ggttgtcgtc atcgaacggt tcatcggact cgtccagcaa cggcaggatc
     6901 acctcacgca gcgccgcttt gctggcgggg ataggtgctg gcagtaattc ttcggtcatc
     6961 accacccggc cagaacgtcc ggccacatat ttcagcgaca tcaaatgctc gtcacggctg
     7021 aaatcagcca gcgcatccgc caccataaac ggtttaatat cgcgcataaa tgcgtcggtt
     7081 gcggtggtca tacagccaat gtgggcatat actcctgtaa taatcagctg gttacgtcca
     7141 ctctctttga gcatttgctc cagcggagaa cgatgaaacg cgctgtagcg ccacttcacc
     7201 agcaccgtgt cgtcggcatc tggcgtcagg cgatccacca ccttttgctg ttccggcgag
     7261 cgggtcaggc ccggccccca catatcattc aacagcgccc gatcttcatc gctctgctct
     7321 ttcggctggg cggtgtaata aaccgggata ttgtgctgtt tgcagtagtc gcgcagcgca
     7381 gcaatattcg cgatcacctg ctccatcatc gggcagttct cgccccagaa gctgacaaaa
     7441 tagtcctgca tatcatggat taacaacgcg gcacgttgcg gttcaaaggc ccagtcaact
     7501 ttattctgcg gaatatcgtg agactccggc agtgcgtaag cctgtaattt tggaatagcc
     7561 atcgtgttct ctccttcagg ctgatgcgcg tgacgccagc cactgacgta attgtttttt
     7621 atcgactttc ccgaccgccg tcagcggaag tgaatccaca cactccacgc gatccggtaa
     7681 tttaaattcg gcaataccct gttcacgcag gaaacgacgc acctgcaccg cgcgcagcgg
     7741 ctcttttacc accagataag cacagctttt ttcgcccatc agctcatttt ccatgctcac
     7801 cagtgcggca tagatcactg ccggatggct cagcagcagg ttttcgatat cttcggcagc
     7861 gatcttctcg ccgccacggt tgatctgatc tttctcgcgc ccctgcacgg tgatgtaacc
     7921 ctctggatca atagagatca gatcgccgga acagtaaaaa ccgttggcat caaaggcact
     7981 ggcattgtgc tgcggacttt tgtaatagcc acggaaggtg tacggcccgc gcgtcatcag
     8041 gcgtccaact tccccttgcg gcagtggatt tccttcggca tcggcaaccc acacttcatc
     8101 gtccggacac attgggtaac cctgggtatg gataattttc tccgcgctat catcaagacg
     8161 ggtgtagttc accagccctt ccgccatgcc aaacacctgc tgcaactggc agccaatctc
     8221 agcgggaata cgcgctgcaa gcgtggcaga aagacgtgcg ccgccgacct gtaacagttt
     8281 cagcgaggca agctgcgccc ggctttcgcc ttcagccagc gcctgcaacc acaggctgac
     8341 tgccggcggc accagcgcgg taacgttaac ctgatgtttt gcaatcaacg ggaagcaaag
     8401 cgtggcgctg ggatcggccg ccagaacaac cgttcctccg gcaagaaaga cgcccagcga
     8461 tcctggcgaa ctcatagtgt agttatgagc cgccgggatc gagcacaggt agcgcgtctg
     8521 ttgtgtgaac tgacaaatct cggcgctacg acgcacgctg tagtaatagt cgttatgagt
     8581 gcgcgggatc agtttcggtg tgccggtggt gccgccggaa agctggaaat aggccacttc
     8641 atcagcaggt gatggcgtgg cagtaaaacc ctctgccgga tggttaatcg catcctgcaa
     8701 gttatgctca ccgctgtcgt tgtgcagttg caccacgcga atggaagaat gctctgcaac
     8761 aaatgtgttg aggaaatcat ccccgctaaa tagcgcatgc tggcgatcgg caatcagtaa
     8821 cgccggttca atctggctgg cgtaggcgtt cagttcacta cgctgatggc tgaacagcgc
     8881 cagcaccggc gcgacgccca gtttcagcag cgcgaaaaag gtaatgtaaa gttcagcgac
     8941 gttacccagt tgtaccagcg cggtttcacc aggtttaatg ccctgacggc gcaaactaca
     9001 ggcgaggtta tccgccgcct gattcagctc ccgataactc aactgccgct cgccgtcgat
     9061 aaccgcgatg ctgtcactcg cagcgtggcg agtcagaatg tcggtcagtg gcaaatcctg
     9121 ccagtagcct ttttcccgat agcgacgggc aaactcttcc ggccagcggg tgaatggaat
     9181 gctcatcctc gctccttaat gcaatccaaa aacgttcaac atggtagaaa gtttgacgcc
     9241 tgtttcgcgc cactcaccca acggtgacga cgcaggcaca atccccgctc cggcaaacag
     9301 acgcacctga ttttcccgca gcttcgcgca gcggatggtc accacccatt cgccgttacc
     9361 ttcgctgtca caccaaccca caatgccgcc aaacagttcg cggtcgaacg gctccagttc
     9421 agcaataacc tgggtcgcgg cctgatgcgg gaaaccgctc agcgcggggg tcggatgcag
     9481 cagacaggcc agagtcagtg cgttttcttg cgaattcgct ttaccttcaa agggagttgc
     9541 gagatgccac agcgtcggcg tggtaatcaa ttgtggagag gagggaacgt gtaactcact
     9601 actgcgttcg cgcagtacct ctttcatcgc ctgagtcccc agttcatgtt catggcgatc
     9661 tttttctgac gccagcagac gattacccgc ttcgcgatcg agcacttcat ccggctgacg
     9721 acgcgcggaa ccggctaacg gaatggagct aaaacgctcg ccgtctttac gtagcagcag
     9781 ttccgggctg gcccccagca ggacgccacc atcagccagc gggacatgga agttgtaact
     9841 aaccgggttt tgcgcaatca accgttccag caatacgcca ctatcaatgg cggcgtcagt
     9901 ggtgatatca atcaatcgtg acaacaccac tttgtcgacc tgcggcgtgg cggtaagtgc
     9961 ggcggcgcgg gcacccatct gttcaaacgt ggtttgttca ggaattgcct ggcgttccac
    10021 cacgttcagc gactggctgc gggtaaaaca gcgggctgag gtctgttttt cctgacggga
    10081 gaacgactgc taggattcgg gaatatacag cgacgaaggc tgacgtggat cgaagggaat
    10141 cgccccgacc atcaccggat ttttgatgcc ctgcgctttg gcatcggcaa acagcgcggc
    10201 gagtttttgc tggaagggac tgtcgggcga atccccgttc acagccggtt catcgaaacg
    10261 ggcgaaacat cctgacgtcg taaaactgcg gtacggcgac ataaagaaaa agcgattggg
    10321 cgcaagtgtt gccatggtct gctgtacttc ctcagccagt gacgtatcca tatcatcctc
    10381 cacaaaatga taaaggcttt aataatgatt atcacttata ttttcggccg ctaacctaaa
    10441 agcaaacgcg cactatgtca actcttgagg taacgcaatc tgcaactcgg ggttgcatct
    10501 ggttgtggtt acaatgaaaa tgagaagcat tattgatgaa ttcgcataag cgcaatgtga
    10561 tgtcctgcgc cgttttgccc cctctcccca aagggacgga ttgtgcgctt tgtcgaattt
    10621 gtcattacgc ccttaacctt attaataaca ggaagctgac ttgtgagact cgccccgctc
    10681 taccgcaacg cccttctatt aacaggactt ttgctttcag gaatagccgt agttcaggcc
    10741 gccgactggc cgcgtcagat tactgacagc cggggcactc ataccctgga aagccagccg
    10801 cagcgtattg tttccaccag cgtcaccctg accggatcac tgctggcgat tgatgctccg
    10861 gtgatcgcca gcggcgcgac cacgccgaat aaccgcgtcg cagatgacca gggcttttta
    10921 cgccagtgga gcaaggtggc gaaagaacgc aaactgcaac ggctctatat cggcgaaccg
    10981 agcgccgaag ccgttgccgc gcaaatgccg gatctgattt taattagcgc aaccggcggg
    11041 gattcggcgc tggcgctgta tgatcagctt tccaccatcg ccccgacatt aatcatcaat
    11101 tacgacgaca aaagctggca gtcgctgtta acgcagcttg gcgaaattac cgggcatgag
    11161 aaacaagcgg cagagcggat tgcacagttt gataagcaac tggcagcggc gaaagagcaa
    11221 atcaaattac cgccgcagcc ggtcactgcc attgtctata ccgccgctgc acacagtgcc
    11281 aatctctgga cgccggaatc agcacaaggg cagatgctgg aacaactcgg ctttacgctg
    11341 gcgaaattgc ccgcagggtt aaacgccagc caaagccagg gcaaacgcca tgacatcatt
    11401 cagcttggtg gggaaaatct ggctgcgggg ttaaacggtg aatcgctatt cctgttcgct
    11461 ggtgatcaga aagatgccga tgcgatttat gccaatccac tgctcgcgca cctgcctgcg
    11521 gtacaaaaca agcaggttta tgcgctggga accgagacgt tccgcctgga ttactacagc
    11581 gccatgcaag tgctggaacg gcttaaggcg ctgttttaag cattaactgt cggacgctgt
    11641 cacctgcggc ggcgtctggc gaaaacgtcg caactccacc agcaccagca ataacaacac
    11701 gccgataatc aacaaaccaa aaccgctcgc gcttgcggaa gcaaccggtg tcatcatcgc
    11761 gcccaaacca cccagcagcg ccgcgcctat ggcatcgccc gtcacgttct gcgccgtcca
    11821 caaaccgtta atccgcccta acatcgcttc cggggtttgc gtttgcagca ttgtatattg
    11881 cagcaacgaa ctaaccgcgc tcagccagcc gaacagcgcc agacaaacca cgcctaaaat
    11941 ccacatcggc atcaggccaa acagaccaat ggcgaggaac gatcccagcg tggagagcag
    12001 catcaataac cctggtcgcg cactatgtgc cagcttcccg ctggttaacg caccaatagc
    12061 cgcgccgagc gggatcgccg cgtagagaaa accaatctgt gccgctgaca tctgccagtt
    12121 gtcagccagc gccggataca gtaccagcac cgcgctcgcc atcgtcaata aaccacccag
    12181 cagcgggctg gcaagcagaa aacgaaatcc tgccagtaat gatttcaacg gatgctcaag
    12241 cggctgcggt ggcggtggca actccggaag gcttaataac ggtaacaagg taataaacgt
    12301 gcccgccgcc gccagcccgt agttccaggc tacgccgccg gtcgccagca ataaaccgcc
    12361 aatcatgggc gaaatcaccg accccaggcg cacggtcaac atggtgatcg ccccggcctg
    12421 cattaagttt tcacgcccta ccagtgcaga tgttgccgcc agtagcgccg taacaccaag
    12481 tgatgcgaaa aaaccatccc ataaaccaag taaatagatt gccagcaatg acggctccgg
    12541 cagcagtgca ttaaggcaca gtccaatgaa gccaatgcca caggtgccgc gcgccagcaa
    12601 aatcactttt ttgcgttcat agcgatccgc cagcacaccg ccaaccatca ggccaacaaa
    12661 catcgcgccg ccggtcagcg tcaccgaaag ccccacctgc caggtagaat gggtcatcat
    12721 ctggatctgc accggcaccg cgacgccgag caaacccaga gacacaattg agataaaacg
    12781 agcgaggaat actgcgcgaa acgccgggtg cgttttcaac aggctgaggt taagcagcca
    12841 ggattgttta ttcattacaa tgccttgcca tcaaatattt taaataccta ttcttaggct
    12901 gcacatgtta acatatccaa ataagatcga taacgataat taatttcatt atcatggaag
    12961 ttcgtatatc tggttctgtt gccgtgacac gcgccattgc cgtgcccgga ttgctgttat
    13021 tactgattat cgcgacggca ttaagcctgc tcattggggc aaaatcactc cccgcttccg
    13081 tagtgctgga ggccttctcc ggcacctgcc agagcgccga ctgcaccatc gtgctcgacg
    13141 cgcggctgcc gcgtaccctt tccggtttac tggcaggcgg cgcgcttggc cttgccgggg
    13201 cgttaatgca aaccctcacc cgaaacccac ttgccgaccc cggcttgctt ggcgtgaacg
    13261 ccggagccag ctttgccatt gtgctgggtg cggcgctgtt tggttactca tccgcacagg
    13321 aacaactggc gatggccttc gccggggcgc tggtggcctc attgattgtt gcctttaccg
    13381 gcagtcaggg tggagggcag ttaagtccgg tgcgtttaac cctggcgggc gtggcgctgg
    13441 cggcggtgct ggaaggactg accagcggca tcgccctgct taatcctgac gtctacgatc
    13501 aactgcgttt ctggcaagcc ggttcgctgg atattcgcaa tctacatacc ttaaaagtgg
    13561 tgctgatccc ggttctgatc gccggagcaa ctgcgctatt actgagtcgc gcgctgaaca
    13621 gtttgagcct cggcagtgac accgcgacgg cgctgggcag tcgcgtggcg cgcacacagt
    13681 tgattggtct gctggcgatt accgtgcttt gtggtagtgc gacggcggta gttggcccga
    13741 ttgcctttat cggcctgatg atgccgcaca tggcgcgctg gctggtaggt gccgatcatc
    13801 gctggtcgct gcccgtcacg ctacttgcta cccctgccct gctgctgttt gccgatatca
    13861 tcgggcgggt gattgtgccc ggcgaactgc gcgtttctgt ggtcagtgcg tttattggtg
    13921 caccggtgct gatcttcctc gtgcgacgta aaacgcgagg tggtgcatga tttacgtctc
    13981 tcgccgatta ctcatcacct gtttgctgct gatttccgcc tgtgtggttg caggtatctg
    14041 gggattacgc agcggtgccg tcacgctgga aatctcgcag gtattcgccg cgttgatggg
    14101 cgatgcgccg cgcagtatga cgatggtggt caccgaatgg cgtttaccac gcgtgctgat
    14161 ggcgctgttg attggcgcgg cgctgggcgt cagcggcgcg atttttcagt cgctgatgcg
    14221 taacccgctc ggcagccctg acgtaatggg ctttaacacc ggggcgtgga gcggcgtgct
    14281 ggtggcgatg gtgctgtttg gtcaggatct gacggctatc gcgctggcag caatggtggg
    14341 cggcattatc acttcgctgt tggtctggct gctcgcctgg cgtaacggta tcgacacctt
    14401 tcggttgatt attatcggta tcggcgttcg cgccatgctg gtggccttta atacctggct
    14461 gttgctgaaa gcgtctttag aaacggcgct aacagcaggt ttgtggaatg ccggatcgct
    14521 caacggcctg acgtgggcaa aaacctcgcc ttccgcaccc atcattatat tgatgctcat
    14581 tgccgccacc ttgctggtgc ggcgaatgcg cttgctggag atgggcgatg ataccgcctg
    14641 cgcgctgggc gtcagcgtcg agcgttcgcg tctgttaatg atgctggttg cagtggtgct
    14701 taccgctgcg gcaacggcgc ttgccgggcc gatttccttt attgctttag tcgcgccgca
    14761 cattgcccga cgcattagcg gcaccgctcg ctgggggctg acccaggcgg cgctgtgcgg
    14821 agcgctgtta ctgctggcgg ccgttctctg cgcccaacaa ctgtttatgc cgtatcaact
    14881 tccggttggc gtcgttaccg tcagcctcgg cggtatttac cttatcgtct tgttaattca
    14941 ggagtctcgc aaaaaatgac cgaatcagta gcccgtttgc gcggcgaaca gttaaccctg
    15001 ggatatggca aatataccgt tgcggaaaat ctgactgtag aaattcctga tggtcacttc
    15061 acggcaatta tcggaccaaa tggctgtggt aaatccacgt tactgcgtac cttaagccgc
    15121 ctgatgacgc ctgctcacgg gcatgtctgg ctggatggcg agcacattca acattacgcc
    15181 agtaaagaag ttgcacgccg gattggtctg ttggcgcaaa atgctaccac gccgggcgat
    15241 atcaccgtgc aggagctggt ggcgcgtgga cgttatccgc atcaaccgct gtttacccgc
    15301 tggcgcaaag aggatgaaga agcggtaacg aaagcgatgc aggccacggg aataactcat
    15361 ctggcagatc aaagcgtgga taccctttct ggcggacaac gccagcgagc gtggatcgcg
    15421 atggtgctgg cccaggaaac ggcaattatg ctgctcgacg aacccacgac ctggctggat
    15481 atcagtcatc agattgatct actggaactg ttaagcgaac tgaaccgcga gaaaggctat
    15541 accctggcgg cggtgctgca cgatcttaat caggcctgtc gttacgccag ccatttgatt
    15601 gcattgcggg aagggaaaat tgttgctcag ggagcgccga aggagattgt cactgctgaa
    15661 ctgattgagc gcatttatgg tctgcgatgc atgatcattg acgatccggt agccggaatg
    15721 ccgcttgtgg tgccgctcgg acgaacggca ccttcaaccg caaatagtta aactaagtgg
    15781 tctgccatca tggcatcctg ttttctggat gccatcgcat agcgcaataa cacgctacca
    15841 caagccacca tcccgccgat cagcgcggaa aggatcacaa taatcgcctt acccggaccg
    15901 tcttttttca ctggcaatga cgggcttaac tgatatttaa acggcgtaaa attcacatcg
    15961 ttgacatttg cttttgttaa ttgctcgaca agatactgcc gattacgtaa ttcaccgttc
    16021 agttccgcaa cgtcagtaac cgctttttca atttccagtt tgcgttcaat accgtctgcg
    16081 ccgagggaaa tagagaaatc ggggtcatct ttaacggcct gaccattact gtatacgggc
    16141 tttttaattc ctgccgcgtt ggcaatgtcg agtgaataat tgaggcgctg aatgtttgca
    16201 tcaagttgat ttttcgtttt aatgcgatcc tgagccagtt tttctttttc aaactgggtt
    16261 ttgatctcca gtttattacg gacgttttct atcgactctt tcaccaccaa cgcagagata
    16321 taatcgatat acccgctcaa aacggtctgc gcctctttac tggttggcgc ggtaaaactt
    16381 agcgtccagg atgtatacag tgacggttca tcttttttct tactagcatt gtcatcaacc
    16441 gctttcattt tttcgctcaa tgcaacaata gcgcgatgca aatccagttc atcgattttc
    16501 gcctctttta attagtccat cacataaggt gatgaacgca ggtactcttc cagcaagcta
    16561 acagactgaa acttcttgat aaacaggtta aatgcaccgc cacggtcgat attaacatcg
    16621 agatcaagaa cgcggagttt ggtaaaggtt ttctccaggt cctgccactg aatggcttcg
    16681 gcgggagtaa taacggctga gctggtccat ttttgcggca ggatgaaaga gatcagtaaa
    16741 cctgcgcagg caaatgcaaa aacgaccgcc atgaccgttt ttttggcccg ccataaaact
    16801 tcgattagat taagtaaatc aatttcatta ttactgggcg acgccagagg atactcagaa
    16861 aaatgagcgt cacttccctg tttaatattc aatgatgaca tggggtataa cctgaaaatg
    16921 agtccaatag gggaaaatct ccacggcgct cactttacca gttggtgaga attatccgaa
    16981 gctgaagttt gtaaattcct tccactgaat gcaacattca ctctatttaa gagaaatatt
    17041 catcaaacct ataaatcaaa caaataaaaa acccataatt attttctgta a
//
LOCUS       DAEBXN010000090        16136 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.090, whole genome shotgun sequence.
ACCESSION   DAEBXN010000090 DAEBXN010000000
VERSION     DAEBXN010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16136
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.090"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(<1..248)
                     /gene="yefM"
                     /locus_tag="KJL99_003025"
     CDS             complement(<1..248)
                     /gene="yefM"
                     /locus_tag="KJL99_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HBD4437670.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDII"
     gene            531..572
                     /gene="hisL"
                     /locus_tag="KJL99_003026"
     CDS             531..572
                     /gene="hisL"
                     /locus_tag="KJL99_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HBD4437671.1"
                     /translation="MTRVQFKHHHHPD"
     misc_feature    548..671
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            718..1617
                     /gene="hisG"
                     /locus_tag="KJL99_003027"
     CDS             718..1617
                     /gene="hisG"
                     /locus_tag="KJL99_003027"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HBD4437672.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPCLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            1623..2927
                     /gene="hisD"
                     /locus_tag="KJL99_003028"
     CDS             1623..2927
                     /gene="hisD"
                     /locus_tag="KJL99_003028"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HBD4437673.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKSIETFHTAQ
                     KLPPVDVETQLGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCDVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETTRQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            2924..3994
                     /gene="hisC"
                     /locus_tag="KJL99_003029"
     CDS             2924..3994
                     /gene="hisC"
                     /locus_tag="KJL99_003029"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HBD4437674.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FKLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIPPYPLSTPVADIAAQALSPQGIVAMSERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            3994..5061
                     /gene="hisB"
                     /locus_tag="KJL99_003030"
     CDS             3994..5061
                     /gene="hisB"
                     /locus_tag="KJL99_003030"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HBD4437675.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVEGYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            5061..5651
                     /gene="hisH"
                     /locus_tag="KJL99_003031"
     CDS             5061..5651
                     /gene="hisH"
                     /locus_tag="KJL99_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HBD4437676.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDIVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            5651..6388
                     /gene="hisA"
                     /locus_tag="KJL99_003032"
     CDS             5651..6388
                     /gene="hisA"
                     /locus_tag="KJL99_003032"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HBD4437677.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            6370..7146
                     /gene="hisF"
                     /locus_tag="KJL99_003033"
     CDS             6370..7146
                     /gene="hisF"
                     /locus_tag="KJL99_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HBD4437678.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVCNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            7140..7751
                     /locus_tag="KJL99_003034"
     CDS             7140..7751
                     /locus_tag="KJL99_003034"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HBD4437679.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWIKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLHKRHQ"
     gene            complement(7937..8914)
                     /gene="wzzB"
                     /locus_tag="KJL99_003035"
     CDS             complement(7937..8914)
                     /gene="wzzB"
                     /locus_tag="KJL99_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HBD4437680.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIEKLKFDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(9060..10180)
                     /gene="ugd"
                     /locus_tag="KJL99_003036"
                     /pseudo
     CDS             complement(9060..10180)
                     /gene="ugd"
                     /locus_tag="KJL99_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
     gene            complement(10430..11836)
                     /gene="gndA"
                     /locus_tag="KJL99_003037"
     CDS             complement(10430..11836)
                     /gene="gndA"
                     /locus_tag="KJL99_003037"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HBD4437681.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLAPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRSALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(11945..12304)
                     /locus_tag="KJL99_003038"
     CDS             complement(11945..12304)
                     /locus_tag="KJL99_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4437682.1"
                     /translation="MNFNSRLESRYSYELMKKASEYSELYGDNLIQLGLEDGIYFYKG
                     MAIGDVFGLARYSDWTISNPECEVIPQDDLIEKMKSFNSSFIVISKRSYANFNPEKYP
                     KFKVLMDTPNGILIAIK"
     gene            complement(12954..13517)
                     /locus_tag="KJL99_003039"
     CDS             complement(12954..13517)
                     /locus_tag="KJL99_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4437683.1"
                     /translation="MVGQFNKIAIFLIFTVCWVLSIIKRQQFRWLAINNIEFSTLFVI
                     LFLVLIFVVTLLSSLRAPGDWDDTMYHLPLARSLVEHHAIVVEQYLRFPLFPQNADLL
                     MALGLQLGDVRLAQFLANICFFVIACGLVGCSWEITKTYYPGIIATILLFTINPLKDH
                     LGYAYIDLTLSLFCCSQYSYIYSLRKQ"
     gene            complement(13724..14662)
                     /locus_tag="KJL99_003040"
                     /pseudo
     CDS             complement(13724..14662)
                     /locus_tag="KJL99_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(14725..15099)
                     /locus_tag="KJL99_003041"
     CDS             complement(14725..15099)
                     /locus_tag="KJL99_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactoprenol-linked glucose translocase"
                     /protein_id="HBD4437684.1"
                     /translation="MLKIGKLLTSSFFSYFLIGIVNTALHWGVFYACYNNLAFGQGRS
                     NIVGFICAATFSFFANARCSFKVSATKARYFIFIFFMGAMSYLFGVLFDLLALSPIFT
                     LFTFSLFSLVLGYCASKYFIFR"
     gene            complement(15103..>16136)
                     /gene="wzy"
                     /locus_tag="KJL99_003042"
     CDS             complement(15103..>16136)
                     /gene="wzy"
                     /locus_tag="KJL99_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen polymerase"
                     /protein_id="HBD4437685.1"
                     /translation="FSFILLIFNLVTFNFSITQKCVSLFIWLLLFPFYGFFVGLLAGN
                     KINDILFDVQPYLFMLSLIYLFTLRYTLKVFSCEIFIKIVNAFALYGSLLYISYIILL
                     NFGLLNFNLIYEHLSLTSEFFFRPDGAFFSKSFYFFGVGAIISFVDKKYLKCLIIVLA
                     ILLTESRGVLLFTTLSLLLASFKLHKLYLNTIIIILGSVLFIIMLYMVGSRSEDSDSV
                     RFNDLYFYYKNVDLATFLFGRGFGSFILDRLRIEIVPLEILQKTGVIGVFISLVPMLL
                     IFLKGYFLNSTKTSLMMSLILFFSITVSITNPFLFTPMGIFIIGVVVLWVFSIENIQI
                     SNNLTSGAK"
BASE COUNT         4629 a         3809 c         3608 g         4090 t
ORIGIN      
        1 tcaataatgt ccttttccgt tcctttgcct gatttcaggc tatcgattga gtccatcaat
       61 ctccgggcgt tagcggggga acgcagtaga taagccgtct cttccagcga attgtattct
      121 tcgagtgaca tcagaacaca agcctctcca ttctgacgag taatgaggat cggggcatga
      181 tcttcaacgg ctttcatcat tgttgccgac aaattctgac gcgcttcgct gtagctaatt
      241 gtacgcatgt aaatctccta ttttgtacag ttcattgtac aatgataggt gttaattaac
      301 tatttattaa ttagtttgta gatcaagaca ttgccagcga gacgaaaaat cagactttgc
      361 tctttttggt gctgtcaagt tagaggacag tcctcttagc cccctccttt ccccgctcat
      421 tcattaaaca aatccatcgc cataaaatat ataaaaaagc ccttgctttc taacgtgaaa
      481 gtggtttagg ttaaaagaca tcagttgaat aaacattcac agagattttt atgacacgcg
      541 ttcaatttaa acaccaccat catcctgact agtctttcag gcgatgtgtg ctggaagaca
      601 ttcagatctt ccagtggtgc atgaacgcat gagaaagccc ccggaagatc accttccggg
      661 ggctttttta ttgcgcggtt gttaacggtt cagacaggtt taaagaggaa taagaaaatg
      721 acagacaaca ctcgtttacg catagctatg cagaaatccg gccgtttaag tgatgactca
      781 cgcgaattac tggcgcgctg tggcattaaa atcaatcttc acacccagcg cctgatcgcg
      841 atggcagaaa acatgccgat tgatattctg cgcgtgcgtg acgatgacat tccctgtctg
      901 gtaatggacg gcgtggtaga ccttgggatt atcggcgaaa acgtgctgga agaagagctg
      961 cttaaccgcc gcgcccaggg tgaagatccg cgctacttta ccctgcgtcg tctggatttc
     1021 ggcggctgcc gcctttcgct ggcaacgccg gttgatgaag cctgggacgg tccgctctcc
     1081 ttaaacggta aacgtatcgc cacctcttat cctcacctgc tcaagcgtta tctcgaccag
     1141 aaaggtatct cttttaaatc ctgcttactg aacggttctg tcgaagttgc gccgcgtgcc
     1201 gggctggcgg atgcgatttg cgatctggtt tccaccggtg ccacgctgga agctaacggc
     1261 ctgcgcgaag tcgaagttat ctaccgctcg aaagcctgcc tgatccagcg cgatggcgaa
     1321 atggaagaat ctaaacagca actgatcgac aagctactga cccgtattca gggtgtgatc
     1381 caggcgcgcg aatcaaaata catcatgatg cacgcaccga ccgaacgcct ggatgaagtc
     1441 atcgccctgc tgccaggtgc cgaacgccca accattctgc cgctggcggg tgatcaacag
     1501 cgcgtagcga tgcacatggt gagtagcgaa accctgttct gggaaacgat ggaaaaactg
     1561 aaagcactgg gtgccagttc aatcctggtc ctgccgattg agaagatgat ggagtgatcg
     1621 ccatgagctt taacacaatc attgactgga atagctgtac tgcggagcaa caacgccagc
     1681 tgttaatgcg cccggcgatc tccgcttctg aaagcattac ccgcaccgtt aacgatattc
     1741 tcgataacgt gaaaacgcgt ggcgatgagg ccctgcgtga atacagcgcg aagtttgata
     1801 aaaccacggt taccgcgctg aaggtgtctg ctgatgagat cgccgccgcc agcgaacgcc
     1861 tgagcgacga gctaaaacag gcgatggcgg tggcagtaaa gagtattgaa accttccaca
     1921 ctgcgcaaaa actgccgccg gtagatgtag aaacgcagct aggcgtgcgt tgccagcagg
     1981 tcacgcgccc ggtggcttca gtgggtttgt atattccggg cggctccgcc ccgctcttct
     2041 caacggtatt aatgctggca actccggcgc gtattgcggg ctgtaaaaaa gtggtgctgt
     2101 gctcaccgcc gccgattgcc gatgaaattc tttatgcggc gcagctgtgc gatgtgcagg
     2161 acgtgtttaa cgtcggcggc gcacaggcca ttgccgcgct ggcgtttggt acggaatctg
     2221 tgccgaaagt ggacaaaatc ttcgggccgg gtaacgcctt tgtcaccgaa gcaaaacgcc
     2281 aggtaagcca gcgtctggac ggtgcggcga tcgatatgcc cgcaggcccg tcggaagtgc
     2341 tggtgattgc tgacagcggc gctacgccgg attttgtggc ttctgatttg ctttctcagg
     2401 ctgaacacgg ccctgactca caggtaattt tactgacccc cgatgctgat atggcgcgtc
     2461 gcgttgccga ggccgtcgaa cgccaactgg cagaactgcc gcgtgccgaa accacccgcc
     2521 aggcactgaa cgccagccgc ctgatcgtga ctaaagattt agcgcagtgc gtggagatct
     2581 ccaaccagta cggcccggag cacctgatca ttcagacccg caacgcccgc gaactggtcg
     2641 atggcatcac cagcgccggt tcggtatttc ttggtgactg gtcaccggaa tcggcaggcg
     2701 actacgcctc cggcaccaac cacgtgctgc cgacttacgg ttacaccgcc acctgttcca
     2761 gcctcgggct ggcagatttc cagaaacgca tgaccgtaca ggaactgtcg aaagaaggtt
     2821 tctccgcgct ggcttcgacc attgaaacac tggccgccgc cgagcgcctg accgcccaca
     2881 aaaatgccgt tactttgcgt gttaacgtcc ttaaggagca agcatgagca ccgtgactat
     2941 tactgattta gcgcgtgaaa acgtccgcaa cctgacaccg tatcagtcgg cgcgtcgtct
     3001 gggcggtaac ggcgacgtct ggctgaacgc caacgaatat cccacagccg tggagtttaa
     3061 gcttactcag caaacgctca accgctaccc ggaatgccag ccaaaagcgg tgattgaaaa
     3121 ttacgcgcaa tatgcaggcg taaaaccgga acaggtgctg gtcagccgtg gcgcggacga
     3181 aggtattgaa ctgctgatcc gcgctttttg cgaaccgggt aaagacgcca tcctctactg
     3241 cccgccaacg tacggcatgt acagcgtcag cgctgaaacc attggtgtcg aatgccgcac
     3301 agtgccgacg ctggaaaact ggcaactgga cttacagggc atttccgaca agctggacgg
     3361 cgtaaaagtg gtctatgttt gcagccccaa caaccccacc gggcaactga tcaatccgca
     3421 ggattttcgc accctgctgg agctaacccg cggtaaggcg attgtggttg ccgatgaagc
     3481 ttatatcgag ttttgcccgc aggcatcgtt agctggctgg ctggcggaat atccgcacct
     3541 ggcgattttg cgtacccttt cgaaagcctt cgcgttggcg gggcttcgtt gcggatttac
     3601 gctggcaaac gaagaagtca tcaacctgct gatgaaagtg atccccccct acccgctctc
     3661 gacgccggtt gccgacattg ccgcacaggc gttaagcccg cagggaatcg tcgccatgag
     3721 cgaacgggtg gcgcaaatta ttacagaacg cgaatacctg attgccgcac tgaaagagat
     3781 cccctgcgtg gagcaggttt tcgactctga aaccaactac attctggcgc gctttaaagc
     3841 ctccagtgca gtgtttaaat ctttgtggga tcagggcatt atcttacgtg atcagaataa
     3901 acaaccctct ttaagcggct gcctgcgaat taccgtcgga acccgtgaag aaagccagcg
     3961 cgtcattgac gccttacgtg cggagcaagt ttgatgagtc agaagtatct ttttatcgat
     4021 cgcgatggaa ccctgattag cgaaccgccg agtgattttc aggtggaccg ttttgacaaa
     4081 ctcgcctttg aaccgggcgt gatcccggaa ttgctgaagc tgcaaaaagc gggctacaag
     4141 ctggtgatga tcactaatca ggatggtctg ggaacacaaa gtttcccgca agcggatttt
     4201 gatggcccgc acaacctgat gatgcagatc ttcacctcgc aaggcgtgca gtttgatgaa
     4261 gtgctgattt gtccgcacct gcccgccgat gagtgcgact gccgtaagcc gaaagtaaaa
     4321 ctggtggagg gttatctggc tgagcaagcg atggatcgtg ccaacagtta tgtaattggc
     4381 gatcgcgcga ccgacattca actggcggaa aacatgggca ttaatggttt acgctacgac
     4441 cgcgaaaccc tgaactggcc aatgattggc gagcaactca ctaaacgaga ccgttacgcc
     4501 catgtagtgc gcaacaccaa agagacgcag attgacgtcc aggtgtggct ggatcgcgaa
     4561 ggtggcagca agattaacac cggcgttggc ttctttgatc acatgctgga tcagatcgcc
     4621 acccacggcg gtttccgcat ggaaatcaat gtcaaaggcg acctctatat cgacgatcac
     4681 cacaccgtcg aagataccgg cctggcgctg ggcgaagcgt taaaaattgc cctcggcgac
     4741 aaacgcggta tttgccgctt tggttttgtg ctgccgatgg acgaatgcct tgcccgctgc
     4801 gcactggata tctctggtcg cccgcacctg gaatataaag ccgagtttac ctaccagcgt
     4861 gtgggcgatc tcagcactga aatgatcgaa cacttcttcc gttcgctctc ttacaccatg
     4921 ggcgtgacac tacacctgaa aaccaaaggt aaaaacgatc atcaccgtgt agaaagcctg
     4981 ttcaaagcct ttggtcgtac cctgcgccag gccatccgcg tcgaaggcga caccctgccc
     5041 tcgtcgaaag gagtgctgta atgaacgtgg tgatccttga tactggctgc gccaacctga
     5101 actctgtgaa gtctgccatt gcgcgccacg gttatgaacc caaagtcagc cgtgacccgg
     5161 acattgtgtt gctggccgat aaactgtttt tacccggcgt cggcaccgca caagcagcga
     5221 tggatcaggt gcgtgagcgc gagctgtttg atctcatcaa agcctgtacc caaccggtgc
     5281 tgggcatctg cttagggatg caactcctgg gacgacgcag cgaagagagc aatggcgtcg
     5341 acttgctggg catcatcgac gaagacgtgc cgaaaatgac cgactttggt ctgccactgc
     5401 cacatatggg ctggaaccgc gtttatccgc aggcaggcaa ccgcctgttt caggggattg
     5461 aagacggcgc gtacttttac tttgttcaca gctacgcaat gccagtcaat ccgtggacca
     5521 tcgcccagtg taattacggc gaaccgttca ccgcggcggt acaaaaagat aacttctacg
     5581 gcgtgcagtt ccacccggag cgttctggtg ccgctggcgc taagttgctg aaaaacttcc
     5641 tggagatgtg atgattattc cggcattaga tttaatcgac ggcaccgtgg tgcgtctcca
     5701 tcagggcgat tacggcaaac aacgcgatta cggtaacgac ccgctgccgc gcttacagga
     5761 ttacgctgcg caaggtgccg aagtgttgca cctggtggat ctgaccgggg caaaagatcc
     5821 ggctaagcgt caaatcccgc tgattaaaac cctggtcgcg ggcgttaacg tcccggtgca
     5881 ggttggcggc ggtgttcgta gtgaagaaga cgtggcggcg ttactggaag ctggcgttgc
     5941 gcgcgtggtg gtcggttcca ccgcagtgaa atcaccggac gtagtgaaag gctggtttga
     6001 acgtttcggt gccgatgcgc tggtgctggc actggatgtc cgtattgacg agcaaggcaa
     6061 caagcaagtg gcagtcagcg gctggcaaga gaactcgggc gtttcactgg aacaactggt
     6121 ggaaacctat ctgcccgtcg gcctgaaaca tgtgttgtgt accgatatct cgcgcgacgg
     6181 cacgctggcg ggatctaacg tctctttata tgaagaagtg tgtgccagat atccgcaagt
     6241 ggcgtttcag tcctccggcg gcattggcga cattaacgat gttgctgccc tacgtggcac
     6301 tggcgtgcgc ggcgtaatag ttggtcgtgc attactggaa ggtaaattca ccgtgaagga
     6361 ggccatcgca tgctggcaaa acgcataatc ccgtgtctcg acgttcgtga tggtcaggtg
     6421 gtgaaaggcg tacagtttcg caaccatgaa atcattggcg atatcgtgcc actggcaaaa
     6481 cgctacgccg aagaaggtgc agatgaactg gtgttctacg atatcaccgc ttccagcgat
     6541 ggccgtgtgg tagataaaag ctgggtatct cgcgtggcgg aagtgatcga cattccgttt
     6601 tgtgtggcgg gtgggattaa gtctctggaa gatgccgcga aaattctttc ctttggcgcg
     6661 gataaaattt ccatcaactc ccctgcgctg gcagacccaa cattaattac tcgcctggcc
     6721 gatcgctttg gcgtgcagtg tattgtggtt ggtattgata cctggtacga cgccgaaacc
     6781 ggtaaatatc atgtaaatca atataccggc gatgaaagcc gcacccgcgt cactcaatgg
     6841 gaaacgctcg actgggtaga ggaggtgcaa aaacgcggcg ccggagaaat cgtcctcaac
     6901 atgatgaatc aggacggcgt gtgtaacggt tacgacctca agcagctgaa aaaagtgcgt
     6961 gaagtttgcc acgtcccgct gattgcctcc ggtggcgcgg gcaccatgga acacttcctg
     7021 gaagccttcc gcgatgccga cgttgacggt gcgctggcag cttccgtatt ccacaaacaa
     7081 ataatcaata ttggcgaatt aaaagcgtac ctggcaacac agggcgtgga gatcaggata
     7141 tgttaacaga acaacaacgt cgcgaactgg actgggaaaa aaccgacggt ctgatgccgg
     7201 tgattgtgca acacgcggta tctggcgaag tgttaatgct gggctacatg aacccggaag
     7261 ccttagacaa aaccatcgaa agcggtaaag tcaccttctt ctcgcgcact aaacagcgac
     7321 tgtggatcaa aggcgaaacc tcgggcaatt ttctcaatgt ggtaagtatt gccccggact
     7381 gcgacaacga cacattactg gtgctggcga atcccatcgg gccaacctgc cacaaaggca
     7441 ccagtagctg cttcggcaac accgctcacc agtggctgtt cctgtatcaa ctggaacaac
     7501 tgctcgccga acgtaaatat gccgacccgg aaacctccta caccgccaaa ctgtatgcca
     7561 gcggcactaa acgcattgcg cagaaagtgg gcgaagaagg cgtggaaacc gcactggcag
     7621 caacggtaca tgatcgcttt gagctgacca acgaggcatc tgacttgatg tatcacctgc
     7681 tggtgctgtt gcaggatcag gatctggatt taacgacagt aattgagaac ctgcataaac
     7741 ggcatcagtg agttacgggc tgagcaggtg tgatgttgtt gtcacacccg gagccattgc
     7801 ccgatgcgca aacttatcgt gcctacacat tatctcaaca cacgtaggcc ggataagatg
     7861 cgccagcatc gcatccggca aaaaaaacgg gcatggtgtc accaccctgc ccgtttctct
     7921 taaatgcaca ataatattac ttcgcgttgt aattacgcag agcgttacgc cccaacacaa
     7981 tccccgcgcc aaccatgccg cccagaagca ctgccagaat caaggtgatt gcctttttcg
     8041 gactatcgcg acgaataggt aacgtcggtt tcatcacata gcggtaagca tgaatatcaa
     8101 gatcatcaaa ctttaatttt tcaatatcca acaggttttg acgtgtctga tagtagtttg
     8161 gtgagaacac caacggacga gtcgcttcat gcttaatcat cgactccagc gcttcgctcc
     8221 ctagaaggaa caacgtatct tgcgtcacat cttcagtctg ctgaacctgt ggctttgtca
     8281 cctgcgcctg attcgcatac tgcaacgctt cctgaatctg acggatacgc agatctttct
     8341 gctcctgcgc gaccacttcc tgggttctta aagagtcctg caagtttttc cgtcccagag
     8401 caatgttgtc cttgagatcc ttttctagct cttgattcac tttatcatca acttgctgaa
     8461 tgtattgggc caacttcatt tgtgcgccct ctgcagtttg cccaacataa gaaacagtca
     8521 atggtaattg ctggttctta acagaaggtt cgatggtaag tttttctggc tcttcctgat
     8581 tatccagcgt ttctgctaat gcagagaagg cagaactgaa gcgaccaatt aacgtctcct
     8641 gcaaatccga tactttcggt gcagcctgac catagataac attcatggca ttgttatagc
     8701 cagcaatttg ccccacgtcg ggctgagtga taattgctgt tgacgtccat ttctccttcg
     8761 ctactgccaa ataaccaata gccagggcaa tagccacaat gacggaaatg ataattgtca
     8821 tcttgccacg ccacaactgc actagtaaat caatcaaatc aatctgttcc gggtcatggt
     8881 tttgcccaga aacattatta ttttctactc tcatcattac cctaactgac ggaaatttcc
     8941 aagtccgcat agtttagcga taatccccgt gaatgctata ggataagtga taaaaataat
     9001 gaattattgc ggaggtaaca aaagacaatc agggcgtaaa tagccctgat aacaagatgt
     9061 tagtcgctgc caaagagatc gcgggtgtag accttatctg ccacatcctt aagctcttct
     9121 gccatacggt tggagataat gacgtcggct tgttgtttga aggtggcgag atcacgttcc
     9181 aggcgagagt tgaagaatga gtcttctttc atcaccggct cgtagatgat cacttcaacg
     9241 cctttcgcct tgatacgctt cataatcccc tgaatagaag acgcacggaa gttatctgac
     9301 ccgctcttca taatcagacg ataaataccc accacttgcg gtttgcatcg acaattgccg
     9361 agatcaggtt attcggcaca gactggtagt tcgccagtaa ctgcttggta tctttcggca
     9421 gacaataacc accataacca aacgacggat tgttgtaatg gttgccaata cgcggatcga
     9481 gacaaacgcc ttcgataatc tggcgagtat tcagacctaa actttctgca taactatcca
     9541 gttcattaaa gtatgctacg cgcatcgcca gataggtatt agcgaaaagt ttaatcgctt
     9601 ctgcttcagt ggagtcggta aacagggtcg ggatattttg cttaatcgcg ccttcctgta
     9661 acaacgcagc aaaacgttct gcgcgttctg aacgctcacc gatgacaata cgtgacggat
     9721 ggagattatc gtaaagggct ttaccttcgc ggaggaattc cggagagaaa ataatatttt
     9781 cagtacgata tttcttatgc atcgctgcgg taaaaccaac gggaaccgtt gatttgatga
     9841 ccataaccgc ataaggattt atctcaacta cgtctttaat tactgattct acactggatg
     9901 tattgaaata attagtttta ggatcatagt cggttggagt ggcgatgatg acataatcag
     9961 catcccggta ggcttcattt ttatctaatg tggcattaaa gtgtatttta tctgattgca
    10021 aaaactgctg aatttcctta tcaacaatag gagatatccg atcattcagc atagcaacgc
    10081 gtgacggtaa aatatctaat gccacaacct catgattttg tgcgattaga agcccgtttg
    10141 acaagcctac atagccagta ccggaaatgg tgattttcat ttcatgctct cagaattaac
    10201 ttaactgtga atcatgatgt ttttagcatc gtgataagat gctaaaagtt ttaacgctac
    10261 gttgtcagat ggtcaacgca acgaaaaaca attcagattt tctttataag aatagctgat
    10321 gtttattaag ttaatattaa gcagtgaaat ttagtctata agatatttgg caaaaaaaag
    10381 cccggtgcaa tatacgccgg gcctcaattt tattgttggt taaatcagat taatccagcc
    10441 attcagtatg gaacacacct tctttatcaa tgcgcttata agtatgcgcc ccgaaatagt
    10501 cacgctgtgc ctggatcagg ttcgcaggca gaaccgcggc acggtagcta tcgtaatagg
    10561 caaccgcagc ggcgaaggtc ggaaccggga taccgttctg tactgcataa gcgacgacat
    10621 cgcgcagcgc ctgctggtag tcatcggcaa tttgcttgaa gtacggggcc agcagcaggt
    10681 tagcgatctg cggattttcg gcataagcat cggtgatttt ctgcaggaac tgcgcacgga
    10741 tgatgcagcc agcacggaaa atcttcgcga tttcgccgta gttcagatcc caattgtatt
    10801 cttcagacgc agcacgcagc tgagagaagc cctgagcgta agaaacgatt ttgcccagat
    10861 acagcgcact gcgaactttt tcgatgaact cagccttgtc gcctgctgac tgagcttgcg
    10921 gaccagagag aactttagat gcggcaacgc gctgatcttt cagagaagag atataacgtg
    10981 caaacacaga ctcggtaatc agcgacagcg gttcgccgag atccagcgcg ctctggctgg
    11041 tccatttacc ggtgcctttg tttgctgctt catccagaat cacatcaacc aggtagttac
    11101 cgtcttcatc ttttttggtg aagatatctt tagtgatgtc gatcaggtag ctgctaagtt
    11161 caccgttatt ccactcggtg aaagtctggg ccagttcttc attagataga ttcaggccac
    11221 ctttgagcag agaatacgct tcagcaatga gctgcatatc tccgtattca ataccattgt
    11281 gaaccatctt cacataatgg cctgcgccgt cggcaccaat ataagtaacg catggctcgc
    11341 cgtcttctgc cacggcagcg attttagtca ggataggcgc aaccagctca taagcctctt
    11401 tctgcccgcc aggcataata gaaggacctt tcagcgcgcc ctcttcaccg ccggaaacac
    11461 cggtaccgat gaagttaaag ccctctgctg aaagctcacg attacgacga atggtgtcct
    11521 ggaagaaggt attaccacca tcaatgatga tgtcaccttt atcgagatat ggtttcaggg
    11581 aatcaatcgc agcatccgtg cctgcacctg ctttcaccat taacaggatg cgacgaggcg
    11641 tttccagaga ttcaacaaac tctttcaccg tatagtaagg agccagtttc ttgcctggat
    11701 tttcagcaat cacttcttcc gtcttttcgc gggagcggtt gaaaatagag acggtataac
    11761 cacgactttc gatgttgagc gcaaggttgc gccccatcac tgccataccg actacgccga
    11821 tctgttgctt tgacattgtt tactcctgtc ttctatatta ttaaccgtct attaaccgtt
    11881 atattattaa ccgtcgtgat tttcatcttc taatcaagga atacatatgg ttacagcatg
    11941 cttcttactt tatagcgata agtatgccat tgggtgtatc cataagaact ttgaattttg
    12001 gatacttttc tggattaaaa ttagcatagc ttcgtttaga tataacaata aacgaactat
    12061 taaaagattt catcttctct attaaatcat cctgtgggat tacctcacac tcgggattac
    12121 taatagtcca atcactatat cttgctaatc caaagacgtc accaattgcc atccctttat
    12181 agaagtaaat accatcctcc aagcctaatt ggataagatt atctccatat aactcactat
    12241 attcacttgc tttcttcatc agttcatagc tatagcgact ttctaagcgt gaattaaaat
    12301 tcaagtattc agaataaaaa tatttgaatt caaaatacaa cgatcgcccc gaaaaaaaaa
    12361 taatacacac aaggataaaa tatcgtccca acttgtaatt tgtaatgttg atatctttta
    12421 ttaaacttgc aattgagatc gcaacctgca atactgacaa tgggtaaatg ggtgataaat
    12481 acctgtcacc ttgcgtaaca gaaaaccaaa ataagacata tgttaaaaca atcgagaaaa
    12541 atacccaaag tattttatta ttcctaagtg taaagacaga cagtatagca taaaaaacaa
    12601 catcactgcc aactttatta attgcttgtt gcaaatctaa tgtggcagga cttataccat
    12661 gtcgagcttg ttctgctgtt tgaacttgta gatcaatttc attccatagg tagtgaccaa
    12721 atatatttcc tccagcagga ctaaaaggat caccagagat ccaaaaacta cggacatacc
    12781 aataaatgcc accaagaatt gttgccaagc taaaaataag aaatgtctgt atgtttctct
    12841 ttttcgctat caatataaca aatatgatgg ctccatatat ggcaccaaat agttttacgc
    12901 cacaagcaat tccaataaaa aaaccacata aaaaaattaa ttcattttgt gttttattgt
    12961 tttctgagcg aatatatata agaatactgg ctgcagcaaa acaaagatag cgttaaatca
    13021 atatatgcat aaccaagatg atctttaagt ggattgatag taaatagtag gattgtagca
    13081 ataatgcctg gataataagt tttcgtaatt tcccatgaac agccaacaag tccacaggct
    13141 attacaaaaa aacagatatt tgctaaaaac tgagctaacc ttacatctcc taactgtaaa
    13201 cctaaagcca ttaaaagatc tgcattttgc gggaacagag gaaaacgcaa atattgttca
    13261 acaacaattg catggtgctc aactaatgaa cgagccaaag gtaagtgata catagtatca
    13321 tcccaatcac cgggagctcg cagggacgat aacaatgtaa ccacaaaaat caaaacaaga
    13381 aataaaataa caaacagcgt actgaactca atattattta ttgccagcca tctaaattgc
    13441 tgacgtttaa taatgcttaa aacccaacac accgtaaaaa ttaaaaagat agcaatctta
    13501 ttaaattgcc cgaccatgcc cagaaagtaa attatcaaaa tatttaaaga catccctatg
    13561 cataggcaat aaaaaataaa gaaaatttgt taagtcctgc caccccctcc ttaaaaaaag
    13621 atgacaatac aatattacca atgacaaaaa atgtcattcc aaaagcaaaa caatacacaa
    13681 catacaaata caacggcaat tcaatcatag taaaatactc tatttacatt atcagtttat
    13741 agtttttttc agaaagataa aataatgcct tcatttcacg tctagttgtt gtatttgaat
    13801 gcaaaataat gccacataca aatgccatta ttgacgataa catgatggag gcagttaaaa
    13861 aggcagtcgg aaatcttctc accaaacctg tatcaatata atcaacaatg atgggcaatc
    13921 ctaaaattat agacaaagca gcaagtaaaa cgccgataat actaaaaaag tatagtggcc
    13981 gttcaagcga gtaaagttta attattgttt tcagaatctt gaacccatca ctccaagttg
    14041 atagtttgct cacactccct tcagatcgct cgccatattt tgtattaacc tcaccatact
    14101 tcatacgtaa ttctaaagca tgaattgtaa gctcagtttc gatttcaaat ccccttgata
    14161 gacatggaaa agatttaaca tagcgtctgg aaaatacacg ataccccgac aacatatcag
    14221 taaaattccc tttaaagata gtctgaacag taccagtcaa caatttattt cctcagcgat
    14281 gcccttttct gtatgtattt tgatctccat tctcttgacg acatcctaca accatatcca
    14341 attgattttt tatgagatgg ttaatcattt ctggagcaga acttgcatca taagtgtcat
    14401 ctccatcaac cattagataa atatccgcat ctacatctga gaacatacgt cttacgacat
    14461 tccccttgcc tttcagagga acagagtgaa ctgttgctcc agcatcctct gccacttgtg
    14521 ctgtactgtc agtcgaatta ttatcaaata catatataga agcagttgga atattcgttt
    14581 tgaaatcatt tatgactttt gctattgcct tctgctcatt ataacaagga ataattacag
    14641 cgatattaaa agagtgatac ataagcccct catataaact atttagctaa tgtgcaagta
    14701 attgtgtttt taacaaatcg tttactaacg aaaaatgaaa tattttgatg cgcaatatcc
    14761 taaaaccaaa ctaaaaagag aaaaggtgaa caatgtaaat attggagata aagctaagag
    14821 atcaaataaa acgccgaata aataactcat cgcccccata aaaaatataa atataaaata
    14881 tctagccttg gttgcagata ctttaaatga gcatcttgca tttgcaaaaa aagaaaaggt
    14941 tgctgcacat ataaaaccaa caatgttact tcggccttga ccaaaagcta aattattata
    15001 acacgcataa aatacccccc aatgtaaagc agtattgaca attccaatta aaaaataact
    15061 aaaaaaagaa ctggtcaata acttccctat ttttaacatc ctttattttg ctccagaagt
    15121 gaggttatta ctaatttgga tattttctat agaaaatacc cataaaacta caacgcctat
    15181 aataaaaatt cccatgggtg taaaaagaaa tggattagtt atagaaacgg taatactgaa
    15241 aaaaagtatt aacgacatca ttaatgatgt ttttgtacta tttaaaaaat agcctttcaa
    15301 aaagataagc aacataggaa ctaatgatat aaatacacca ataacgcctg ttttctgaag
    15361 tatctcaaga ggtactattt caatccttaa tcgatctaat ataaatgaac caaatcctct
    15421 tccaaacaag aacgtcgcta aatcaacatt tttataataa aaatataaat cattaaatct
    15481 aacagagtca gaatcttcac tgcgtgatcc gaccatgtaa agcataatta taaatagaac
    15541 gctgcccaat attattataa tagtatttaa atatagctta tgtaatttaa aactggctaa
    15601 taacagtgat aatgttgtaa aaagtaatac acctcttgat tctgtcaata atatcgcaag
    15661 cactattatg agacatttta aatatttttt gtcgacaaaa ctgataatcg caccgacacc
    15721 aaaaaagtag aaggatttgg aaaaaaaagc cccatcggga cgaaaaaaga actcgctagt
    15781 caatgataag tgttcataaa ttaaattaaa atttaacaaa ccgaaattca acaaaattat
    15841 gtatgaaata tataacagtg atccatataa tgcaaatgca ttaactattt taataaaaat
    15901 ctcacatgaa aatactttta aagtatatct tagtgtaaat agatatataa gtgacagcat
    15961 aaaaaggtat ggttgcacat caaacagtat atcatttatt ttattaccag ctaataagcc
    16021 gacaaagaag ccataaaaag gaaataaaag caaccatata aaaagactga cacatttttg
    16081 ggtgattgaa aaattgaatg taactaaatt aaaaattaaa agtataaaac taaata
//
LOCUS       DAEBXN010000091        16096 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.091, whole genome shotgun sequence.
ACCESSION   DAEBXN010000091 DAEBXN010000000
VERSION     DAEBXN010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16096
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.091"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..588
                     /gene="tuf"
                     /locus_tag="KJL99_003043"
     CDS             <1..588
                     /gene="tuf"
                     /locus_tag="KJL99_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBD4437686.1"
                     /translation="IPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEV
                     EIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKP
                     HTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKM
                     VVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKVLG"
     gene            771..965
                     /gene="bfd"
                     /locus_tag="KJL99_003044"
     CDS             771..965
                     /gene="bfd"
                     /locus_tag="KJL99_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HBD4437687.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            1038..1514
                     /gene="bfr"
                     /locus_tag="KJL99_003045"
     CDS             1038..1514
                     /gene="bfr"
                     /locus_tag="KJL99_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HBD4437688.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(1511..1978)
                     /locus_tag="KJL99_003046"
                     /pseudo
     CDS             complement(1511..1978)
                     /locus_tag="KJL99_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
     gene            2357..2668
                     /gene="rpsJ"
                     /locus_tag="KJL99_003047"
     CDS             2357..2668
                     /gene="rpsJ"
                     /locus_tag="KJL99_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HBD4437689.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2701..3330
                     /gene="rplC"
                     /locus_tag="KJL99_003048"
     CDS             2701..3330
                     /gene="rplC"
                     /locus_tag="KJL99_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HBD4437690.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            3341..3946
                     /gene="rplD"
                     /locus_tag="KJL99_003049"
     CDS             3341..3946
                     /gene="rplD"
                     /locus_tag="KJL99_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HBD4437691.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLQKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3943..4245
                     /gene="rplW"
                     /locus_tag="KJL99_003050"
     CDS             3943..4245
                     /gene="rplW"
                     /locus_tag="KJL99_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HBD4437692.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            4263..5084
                     /gene="rplB"
                     /locus_tag="KJL99_003051"
     CDS             4263..5084
                     /gene="rplB"
                     /locus_tag="KJL99_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HBD4437693.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            5101..5379
                     /gene="rpsS"
                     /locus_tag="KJL99_003052"
     CDS             5101..5379
                     /gene="rpsS"
                     /locus_tag="KJL99_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HBD4437694.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            5394..5726
                     /gene="rplV"
                     /locus_tag="KJL99_003053"
     CDS             5394..5726
                     /gene="rplV"
                     /locus_tag="KJL99_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HBD4437695.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VQVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5744..6445
                     /gene="rpsC"
                     /locus_tag="KJL99_003054"
     CDS             5744..6445
                     /gene="rpsC"
                     /locus_tag="KJL99_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HBD4437696.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            6458..6868
                     /gene="rplP"
                     /locus_tag="KJL99_003055"
     CDS             6458..6868
                     /gene="rplP"
                     /locus_tag="KJL99_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HBD4437697.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6868..7059
                     /gene="rpmC"
                     /locus_tag="KJL99_003056"
     CDS             6868..7059
                     /gene="rpmC"
                     /locus_tag="KJL99_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HBD4437698.1"
                     /translation="MKAKELREKSIEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            7059..7313
                     /gene="rpsQ"
                     /locus_tag="KJL99_003057"
     CDS             7059..7313
                     /gene="rpsQ"
                     /locus_tag="KJL99_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HBD4437699.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            7478..7849
                     /gene="rplN"
                     /locus_tag="KJL99_003058"
     CDS             7478..7849
                     /gene="rplN"
                     /locus_tag="KJL99_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HBD4437700.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7860..8174
                     /gene="rplX"
                     /locus_tag="KJL99_003059"
     CDS             7860..8174
                     /gene="rplX"
                     /locus_tag="KJL99_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HBD4437701.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIIEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            8189..8728
                     /gene="rplE"
                     /locus_tag="KJL99_003060"
     CDS             8189..8728
                     /gene="rplE"
                     /locus_tag="KJL99_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HBD4437702.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8743..9048
                     /gene="rpsN"
                     /locus_tag="KJL99_003061"
     CDS             8743..9048
                     /gene="rpsN"
                     /locus_tag="KJL99_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HBD4437703.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            9082..9474
                     /gene="rpsH"
                     /locus_tag="KJL99_003062"
     CDS             9082..9474
                     /gene="rpsH"
                     /locus_tag="KJL99_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HBD4437704.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            9487..10020
                     /gene="rplF"
                     /locus_tag="KJL99_003063"
     CDS             9487..10020
                     /gene="rplF"
                     /locus_tag="KJL99_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HBD4437705.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            10030..10383
                     /gene="rplR"
                     /locus_tag="KJL99_003064"
     CDS             10030..10383
                     /gene="rplR"
                     /locus_tag="KJL99_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HBD4437706.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            10398..10901
                     /gene="rpsE"
                     /locus_tag="KJL99_003065"
     CDS             10398..10901
                     /gene="rpsE"
                     /locus_tag="KJL99_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HBD4437707.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10905..11084
                     /gene="rpmD"
                     /locus_tag="KJL99_003066"
     CDS             10905..11084
                     /gene="rpmD"
                     /locus_tag="KJL99_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HBD4437708.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            11088..11522
                     /gene="rplO"
                     /locus_tag="KJL99_003067"
     CDS             11088..11522
                     /gene="rplO"
                     /locus_tag="KJL99_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HBD4437709.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11530..12861
                     /gene="secY"
                     /locus_tag="KJL99_003068"
     CDS             11530..12861
                     /gene="secY"
                     /locus_tag="KJL99_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HBD4437710.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12893..13009
                     /gene="rpmJ"
                     /locus_tag="KJL99_003069"
     CDS             12893..13009
                     /gene="rpmJ"
                     /locus_tag="KJL99_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HBD4437711.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            13156..13512
                     /gene="rpsM"
                     /locus_tag="KJL99_003070"
     CDS             13156..13512
                     /gene="rpsM"
                     /locus_tag="KJL99_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HBD4437712.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13529..13918
                     /gene="rpsK"
                     /locus_tag="KJL99_003071"
     CDS             13529..13918
                     /gene="rpsK"
                     /locus_tag="KJL99_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HBD4437713.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13952..14572
                     /gene="rpsD"
                     /locus_tag="KJL99_003072"
     CDS             13952..14572
                     /gene="rpsD"
                     /locus_tag="KJL99_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HBD4437714.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14598..15587
                     /gene="rpoA"
                     /locus_tag="KJL99_003073"
     CDS             14598..15587
                     /gene="rpoA"
                     /locus_tag="KJL99_003073"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HBD4437715.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15628..16011
                     /gene="rplQ"
                     /locus_tag="KJL99_003074"
     CDS             15628..16011
                     /gene="rplQ"
                     /locus_tag="KJL99_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HBD4437716.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
BASE COUNT         4141 a         3784 c         4292 g         3879 t
ORIGIN      
        1 attccggaac cagagcgtgc gattgacaag ccgttcctgc tgccgatcga agacgtattc
       61 tccatctccg gtcgtggtac cgttgttacc ggtcgtgtag aacgcggtat catcaaagtt
      121 ggtgaagaag ttgaaatcgt tggtatcaaa gagactcaga agtctacctg tactggcgtt
      181 gaaatgttcc gcaaactgct ggacgaaggc cgtgctggtg agaacgtagg tgttctgctg
      241 cgtggtatca aacgtgaaga aatcgaacgt ggtcaggtac tggctaagcc gggcaccatc
      301 aagccgcaca ccaagttcga atctgaagtg tacattctgt ccaaagatga aggcggccgt
      361 catactccgt tcttcaaagg ctaccgtccg cagttctact tccgtactac tgacgtgact
      421 ggtaccatcg aactgccgga aggcgtagag atggtaatgc cgggcgacaa catcaaaatg
      481 gttgttaccc tgatccaccc gatcgcgatg gacgacggtc tgcgtttcgc aatccgtgaa
      541 ggcggccgta ccgttggcgc gggcgttgtt gctaaagttc tgggctaatt acacgttaat
      601 tagttttgaa ttgaaaaggg cgcttcggcg ccctttttgc tgcccgtaat ttgagctcga
      661 aatatttagt cgtaattttg ctcgctgaac tatggcgcaa ataaaactgt gctattgtaa
      721 tcataaccat tctcatttac actttgtgca gaatttgaac gggagcaatc atgtacgttt
      781 gtttgtgtaa tggtgtaagt gacaaaaaaa ttcgccaggc agtccgtcag tttcatccgc
      841 aatctttcca acaattgcgt aagtttattc ctgtgggaaa tcaatgcggt aagtgcattc
      901 gcgccgcgcg cgaagtgatg caggacgagt tgatgcagat gccagaattt aaagagattg
      961 cctgaagccc acgcttattt tttgacatcc tgctagcccc atctacgctc taatagtgga
     1021 agcggaggga ctataaaatg aaaggtgatg ttaaaatcat aaattatctc aataaactat
     1081 tgggaaatga gcttgtcgca atcaatcagt attttctcca tgcccggatg tttaaaaact
     1141 ggggcctgat gcgtctcaat gacgtcgaat accatgaatc catcgatgag atgaaacacg
     1201 ctgataaata tatcgagcgt attttgtttt tagaaggaat ccccaactta caggatttag
     1261 gcaaactggg gattggtgaa gatgtcgagg agatgctgca atctgacctg cgattagaac
     1321 tggaaggcgc gaaggatctg cgtgaggcta tcgcatatgc cgacagcgtt catgactatg
     1381 ttagccgcga tatgatgatc gagatccttg ctgatgaaga aggtcatatc gactggctcg
     1441 aaactgaact ggatctcatc ggtaaaatcg gcctacaaaa ttacctgcaa tcacaaatta
     1501 aagttaaaga ttaacggcct atcagcaaac tatcccagcc tataacgaaa cccgcagccg
     1561 ccagaaatgg cccaaaaggc agcgggtttt ttaatgattc ttttcctctc atgagcagac
     1621 caataactac ggctccgcaa gcgaatgagg cagcaaggaa aaccagtcgt ggtaagaagg
     1681 cccatgaatg ctaggcgcca agggcggcga gaaacttcac atcaccatag cctaatcctt
     1741 ctttatgtcg cagtatgcga tacccccagt aaatgacagc gaatgtgccg taaccaacaa
     1801 ttgcccccca taacgcatcg gctaaaccat caggatgaca aacttgatag aacaaaagac
     1861 ctgaccagag taacgggcac gtaaatctgt caggtaacaa gccatgcttt gcatcccaga
     1921 aaaaaagtag ggctgataaa caagcgtaca aaattagaaa gggaagggta gtgtccataa
     1981 aacatcctct gtaattggag tatcaccatc gaataactcc ggatgctcag caacagagag
     2041 agcgctgatt tgttaacctg attccggtga aaaacgctat cttcacaacc ttacaaattt
     2101 cgtggaatgc agctcgcaat ctgcgcgttg cgattaattt ctacccgatg cttgcgtcag
     2161 gctcagattt gcgtataatg cgcgggcttg tcgtagttga cagcaggttc aatctgaacc
     2221 tcagttagcc gaatttggct acctaacaat gctcccaatc ggggagctac gtaagaacgg
     2281 ttacactctc ccatcaatcg taatgggtct gaggagtaat cattttcgtt tataaaataa
     2341 ttggagctct ggtctcatgc agaaccaaag aatccgtatc cgcctgaaag cgtttgatca
     2401 tcgtctgatc gatcaagcaa ccgcggaaat cgtcgagact gccaagcgca ctggtgcgca
     2461 ggtccgtggt ccgatcccgc tgccgacacg caaagagcgc ttcactgttc tgatctcccc
     2521 gcacgtcaac aaagacgcgc gcgatcagta cgaaatccgt actcacttgc gtctggttga
     2581 catcgttgag ccaaccgaga aaaccgttga tgctctgatg cgtctggatc tggctgccgg
     2641 tgtagacgtg cagatcagcc tgggttaatc aggtcattga gcgattgaga ggttgaaaca
     2701 atgattggtt tagtcggtaa aaaagtgggt atgacccgta tcttcacaga agacggcgtt
     2761 tctatcccag taaccgtaat cgaagttgaa gcaaaccgcg ttactcaggt taaagacctg
     2821 gctaacgatg gctaccgtgc tattcaggtg accaccggtg ctaaaaaagc taaccgtgtg
     2881 accaagcctg aagctggcca cttcgctaaa gctggcgtag aagctggccg tggtctgtgg
     2941 gaattccgcc tggctgaagg cgaagagttc actgtaggtc agagcattag cgttgaactg
     3001 tttgctgacg ttaaaaaagt tgacgtaact ggcacctcta aaggtaaagg tttcgcaggt
     3061 accgttaagc gctggaactt ccgtacccag gacgctactc acggtaactc cttgtctcac
     3121 cgcgttccgg gttctatcgg tcagaaccag actccgggca aagtgttcaa aggcaagaaa
     3181 atggcaggtc agatgggtaa cgaacgtgta accgttcaga gccttgacgt agtacgcgtt
     3241 gacgctgagc gcaacctgct gctggttaaa ggtgctgtcc cgggtgcaac cggtagcgac
     3301 ctgatcgtta aaccagctgt gaaggcgtaa ggagatagca atggaattag tattgaaaga
     3361 cgcgcagagc gcgctgactg tttccgaaac taccttcggt cgtgatttca acgaagcgct
     3421 ggttcaccag gttgttgttg cttatgcagc tggtgctcgt cagggtactc gtgctcagaa
     3481 gactcgtgct gaagtaactg gttccggtaa aaaaccgtgg cgccagaaag gcaccggccg
     3541 tgcgcgttct ggttctatca agagcccgat ctggcgttct ggtggcgtga cctttgctgc
     3601 tcgtccgcag gaccacagtc aaaaagttaa caagaagatg taccgcggcg cgctgaaaag
     3661 catcctgtcc gaactggtac gtcaggatcg tctgatcgtt gtcgagaagt tctctgtaga
     3721 agcgccgaaa actaagctgc tggcacagaa actgaaagac atggctctgg aagatgtgct
     3781 gatcatcacc ggtgagctgg acgaaaacct gttcctggct gcgcgcaacc tgcagaaggt
     3841 tgacgtacgc gatgcaactg gtatcgaccc ggttagcctg atcgccttcg acaaagtcgt
     3901 aatgactgct gatgctgtta agcaagttga ggagatgctg gcatgattcg tgaagaacgt
     3961 ctgctgaagg tgctgcgtgc accgcacgtt tctgaaaaag cgtctactgc gatggaaaaa
     4021 tccaacacca tcgtactcaa agttgctaaa gacgcgacca aagcagaaat caaagctgct
     4081 gtgcagaaac tgtttgaagt cgaagtcgaa gtcgttaaca ccctggtagt taaagggaaa
     4141 gttaaacgtc acggacagcg tatcggtcgt cgtagcgact ggaaaaaagc ttacgtcacc
     4201 ctgaaagaag gccagaatct ggacttcgtt ggcggcgctg agtaagtcgg aggagtaata
     4261 caatggcagt tgttaaatgt aaaccgacat ctccgggtcg tcgccacgta gttaaagtgg
     4321 ttaaccctga gctgcacaag ggcaaacctt ttgctccgtt gctggaaaaa aacagcaaat
     4381 ccggtggtcg taacaacaat ggccgtatca ccactcgtca tatcggtggt ggccacaagc
     4441 aggcttaccg tattgttgac ttcaaacgca acaaagacgg tatcccggca gttgttgaac
     4501 gtcttgagta cgatccgaac cgttccgcga acatcgcgct ggttctgtac aaagacggtg
     4561 aacgccgtta catcctggcc cctaaaggcc tgaaagctgg cgaccagatt cagtctggcg
     4621 ttgatgctgc aatcaaacca ggtaacaccc tgccgatgcg caacatcccg gttggttcta
     4681 ctgttcataa cgtagaaatg aaaccaggta aaggcggtca gctggcacgt tccgctggta
     4741 cttacgttca gatcgttgct cgtgatggtg cttatgtcac cctgcgtctg cgttctggtg
     4801 aaatgcgtaa agtagaagca gactgccgtg caactctggg cgaagttggc aatgctgagc
     4861 atatgctgcg cgttctgggt aaagcaggtg ctgcacgctg gcgtggtgtt cgtccgaccg
     4921 ttcgcggtac cgcgatgaac ccggtagacc acccacatgg tggtggtgaa ggtcgtaact
     4981 ttggtaagca cccggtaact ccgtggggcg ttcagaccaa aggtaagaag acccgcagca
     5041 acaagcgtac tgataaattc atcgtacgtc gccgtagcaa ataattttag aggataagcc
     5101 atgccacgtt ctctcaagaa aggtcctttt attgacctgc acttgctgaa gaaggtagag
     5161 aaagcggtgg aaagcggaga caagaagccc ctgcgcactt ggtcccgtcg ttcaacgatc
     5221 tttcctaaca tgatcggttt gaccatcgct gtccataatg gtcgtcagca cgttccggta
     5281 tttgtaaccg acgaaatggt cggtcacaaa ctgggtgaat tcgcaccgac tcgtacttat
     5341 cgcggccacg ctgctgataa aaaagcgaag aagaaataag gtaggaggaa gagatggaaa
     5401 ctattgctaa acatcgccat gctcgttctt ctgctcagaa ggttcgcctt gttgctgacc
     5461 tgattcgcgg taagaaagtg tcgcaggctc tggatatttt gacctacacc aacaagaaag
     5521 cggctgtaca ggtcaagaaa gttctggaat ctgccattgc taacgctgaa cacaacgatg
     5581 gcgctgacat tgacgatctg aaagttacga aaattttcgt agacgaaggc ccgagcatga
     5641 agcgcattat gccgcgtgca aaaggtcgtg cagatcgcat cctgaagcgc accagccaca
     5701 tcactgtggt tgtgtccgat cgctgagact ctggagacta gcaatgggtc agaaagtaca
     5761 tcctaatggt attcgcttgg gtattgtaaa accatggaac tctacttggt ttgcgaacac
     5821 caaagaattc gctgacaacc tggacagcga ttttaaagta cgtcagtacc tgactaagga
     5881 actggctaaa gcgtccgtat ctcgtatcgt tatcgagcgt ccggctaaga gcatccgtgt
     5941 aaccattcac actgctcgcc cgggtatcgt tatcggtaaa aaaggtgaag acgtagaaaa
     6001 actgcgtaag gtcgtagcgg acatcgctgg cgttcctgca cagatcaaca tcgccgaagt
     6061 tcgtaagcct gaactggacg caaaactggt tgctgacagc atcacttctc agctggaacg
     6121 tcgcgttatg ttccgtcgtg ctatgaagcg tgctgtacag aacgcaatgc gtctgggcgc
     6181 taaaggtatt aaagttgaag ttagcggccg tctgggcggc gcggaaatcg cacgtaccga
     6241 atggtaccgc gaaggtcgcg taccgctgca cactctgcgt gctgacatcg actacaacac
     6301 ctctgaagcg cacaccactt acggtgtaat cggcgttaaa gtgtggatct tcaaaggcga
     6361 gatcctgggt ggtatggctg ctgttgaaca accggaaaaa ccggctgctc agcctaaaaa
     6421 gcagcagcgt aaaggccgta aataaggagc gtcgctgatg ttacaaccaa agcgtacaaa
     6481 attccgtaaa atgcacaaag gccgtaaccg cggtctggcg cagggtacgg atgttagctt
     6541 cggcagcttc ggtctgaaag ctgttggccg tggtcgtctg actgcccgtc agatcgaagc
     6601 agcacgtcgt gctatgaccc gtgcagttaa gcgtcaaggt aagatctgga tccgtgtgtt
     6661 cccggacaaa ccgatcactg aaaagccgct ggcagtgcgt atgggtaaag gtaaaggtaa
     6721 cgtggagtat tgggttgcct tgattcagcc gggtaaagtc ctgtatgaaa tggacggtgt
     6781 tccggaagag ctggcccgtg aagcattcaa gctggcagca gcgaaactgc cgattaaaac
     6841 cacctttgta actaagacgg tgatgtaatg aaagcaaaag agctgcgtga gaagagcatt
     6901 gaagagctga acaccgagct gctgaacctg ctgcgtgagc agttcaacct gcgtatgcag
     6961 gctgcaagtg gccagctgca acagtctcac ctgttgaagc aagtgcgtcg cgatgtcgca
     7021 cgcgttaaga ctttactgaa cgagaaggcg ggtgcgtaat gaccgataaa atccgtactc
     7081 tgcaaggtcg cgttgttagc gacaaaatgg agaaatccat tgttgttgct atcgaacgtt
     7141 ttgtgaaaca cccgatctac ggaaaattca tcaagcgtac gaccaaactg cacgtacatg
     7201 acgagaacaa cgaatgcggt atcggtgacg tggttgaaat ccgcgaatgc cgtccgctgt
     7261 ccaagactaa atcctggacg ctggttcgcg ttgtagagaa agcggttctg taatacagta
     7321 cactctctcg atacgaataa acggctcaga aatgagccgt ttattttttc tacccatatc
     7381 cttgaagcgg tgttataatg ccgcgccctc gatatgggga tttttaacga cctgattttc
     7441 gggtctcagt agtagttgac attagcggag cactaaaatg atccaagaac agactatgct
     7501 gaacgtcgcc gacaactccg gtgcacgtcg cgtaatgtgt atcaaggttc tgggtggctc
     7561 gcaccgtcgc tacgcaggcg taggcgacat catcaagatc accatcaaag aagcaattcc
     7621 gcgtggtaag gtcaaaaaag gtgatgtgct gaaggcggta gtggtgcgca ccaagaaggg
     7681 tgttcgtcgc ccggacggtt ctgtcattcg cttcgatggt aatgcttgtg ttcttctgaa
     7741 caacaacagc gagcagccta tcggtacgcg tatttttggg ccggtaactc gtgagcttcg
     7801 tagtgagaag ttcatgaaaa ttatctctct ggcaccagaa gtactctaag gagcgaatca
     7861 tggcagcgaa aatccgtcgt gatgacgaag ttatcgtgtt aaccggtaaa gataaaggta
     7921 aacgcggtaa agttaagaat gtcctgtctt ccggcaaggt cattattgaa ggtatcaacc
     7981 tggttaagaa acatcagaag ccggttccgg ccctgaacca accgggtggc atcgttgaaa
     8041 aagaagccgc tattcaggtt tccaacgtag caatcttcaa tgcggcaacc ggcaaggctg
     8101 accgtgtagg ctttagattc gaagacggca aaaaagtccg tttcttcaag tctaacagcg
     8161 aaactatcaa gtaatttgga gtagtacgat ggcgaaactg catgattact acaaagacga
     8221 agtagttaaa aaactcatga ctgagtttaa ctacaattct gtcatgcaag tccctcgggt
     8281 cgagaagatc accctgaaca tgggtgttgg tgaagcgatc gctgacaaaa aactgctgga
     8341 taacgcagca gcagacctgg cagcaatctc cggtcaaaaa ccgctgatca ccaaagcacg
     8401 caaatctgtt gcaggcttca aaatccgtca gggctatccg atcggctgta aagtaactct
     8461 gcgtggcgaa cgcatgtggg agttctttga gcgcctgatc actattgctg tacctcgtat
     8521 ccgtgacttc cgtggcctgt ccgctaagtc tttcgacggt cgtggtaact acagcatggg
     8581 tgtccgtgag cagatcatct tcccagaaat cgactacgat aaagtcgacc gcgttcgtgg
     8641 tttggatatt accattacca ctactgcgaa atctgacgaa gaaggccgcg ctctgctggc
     8701 tgcctttgac ttcccgttcc gcaagtaagg tagggttact aaatggctaa gcaatcaatg
     8761 aaagcacgcg aagtaaaacg cgtagcttta gctgataaat acttcgcgaa acgcgctgaa
     8821 ctgaaagcga tcatctctga tgtgaacgct tccgacgaag atcgttggaa cgctgttctc
     8881 aagctgcaga ctctgccgcg tgattccagc ccgtctcgtc agcgtaaccg ctgccgtcaa
     8941 acaggtcgtc cgcatggttt cctgcggaag ttcgggttga gccgtattaa ggtccgtgaa
     9001 gccgctatgc gcggtgaaat cccgggtctg aaaaaggcta gctggtaatt gtcaccaatt
     9061 gaatcacggg agtaaagaca gatgagcatg caagatccga tcgcggatat gctgacccgt
     9121 atccgtaacg gtcaggccgc gaacaaagct gcggtcacca tgccttcctc caagctgaaa
     9181 gtggcaatcg ccaacgtgct gaaggaagaa ggttttattg aagattttaa agttgaaggc
     9241 gacaccaagc ctgaactgga acttactctg aagtatttcc agggcaaagc tgttgtagaa
     9301 agcattcagc gtgtcagccg cccaggtctg cgcatctata aacgtaaaga tgagctgccg
     9361 aaagttatgg cgggtctggg tatcgcagtt gtttctacct ctaaaggtgt tatgactgat
     9421 cgtgcagcgc gccaggctgg tcttggtggc gaaattatct gctacgtagc ctaatcggag
     9481 gaaaaaatgt ctcgtgttgc taaagcaccg gtcgttgttc ctgccggcgt tgacgtaaaa
     9541 atcaacggtc aggttattac gatcaaaggt aaaaacggcg agctgactcg tactctcaac
     9601 gatgctgttg aagttaaaca tgcagataat accctgacct tcggtccgcg tgatggttac
     9661 gcagacggtt gggcacaggc tggtaccgcg cgtgccctgc tgaactcaat ggttatcggt
     9721 gttaccgaag gcttcactaa gaagctgcag ctggttggtg taggttaccg tgcagcggtt
     9781 aaaggcaatg tgattaacct gtctctgggt ttctctcatc ctgttgacca tcagctgcct
     9841 gcgggtatca ctgctgaatg tccgactcag actgaaatcg tgctgaaagg cgctgataag
     9901 caggtgatcg gccaggttgc agcggatctg cgcgcctacc gtcgtcctga gccttacaaa
     9961 ggcaaggggg ttcgttacgc cgacgaagtc gtgcgtacca aagaggctaa gaagaagtaa
    10021 ggtaacacta tggataagaa atctgctcgt atccgtcgtg cgacccgcgc acgccgcaag
    10081 ctccaggagc tgggcgcaac tcgcctggtg gtacatcgta ccccgcgtca catttacgca
    10141 caggtaattg caccgaacgg ttctgaagtt ctggtagctg cttctactgt agaaaaagct
    10201 atcgctgaac aactgaagta caccggtaac aaagacgcgg ctgcagctgt gggtaaagct
    10261 gtcgctgaac gcgctctgga aaaaggcatc aaagatgtat cctttgaccg ttccgggttc
    10321 caatatcatg gtcgtgtcca ggcactggca gatgctgccc gtgaagctgg ccttcagttc
    10381 taaggtagag gtgtaagatg gctcacatcg aaaaacaagc tggcgaactg caggaaaagc
    10441 tgatcgcggt aaaccgcgta tctaaaaccg ttaaaggtgg tcgtattttc tccttcacag
    10501 ctctgactgt agttggcgat ggtaacggtc gcgttggttt tggttacggt aaagcgcgtg
    10561 aagttccagc agcgatccag aaagcgatgg aaaaagcccg tcgcaatatg attaacgtcg
    10621 cgctgaataa cggcactcta caacaccctg ttaaaggtgt tcacacgggt tctcgcgtat
    10681 tcatgcagcc ggcttccgaa ggtaccggta tcatcgccgg tggtgcaatg cgcgccgttc
    10741 tggaagtcgc tggggttcat aacgttctgg ctaaagcata tggttccacc aacccgatca
    10801 acgtggttcg tgcaactatt gatggcctgg aaaatatgaa ttctccagaa atggtcgctg
    10861 ccaagcgtgg taaatccgtt gaagaaattc tggggaaata aaccatggca aagactatta
    10921 aaattactca aacccgcagt gcaatcggtc gtctgccgaa acacaaggca acgctgcttg
    10981 gcttgggtct gcgtcgtatt ggtcacaccg tagagcgcga ggatactcct gctattcgcg
    11041 gtatgatcaa cgcggtttcc ttcatggtta aagttgagga gtaagagatg cgtttaaata
    11101 ctctgtctcc ggccgaaggc tccaaaaagg cgggtaaacg cctgggtcgt ggtatcggtt
    11161 ctggcctcgg taaaaccggt ggtcgtggtc acaaaggtca gaagtctcgt tctggcggtg
    11221 gcgtacgtcg cggtttcgag ggtggtcaga tgcctctgta ccgtcgtctg ccgaaattcg
    11281 gcttcacttc tcgtaaagca gcgattacag ccgaagttcg tctatctgac ctggctaaag
    11341 tagaaggcgg tgttgtagac ctgaacacgc tgaaagcggc taacattatc ggtatccaga
    11401 tcgagttcgc gaaagtgatc ctggctggcg aagtaacgac tccggtaact gttcgtggcc
    11461 tgcgtgttac taaaggcgct cgtgctgcta tcgaagctgc tggcggtaaa atcgaggaat
    11521 aagtagcaga tggctaaaca accgggatta gattttcaaa gtgccaaagg tggcttaggc
    11581 gagctgaaac gcagactgct gtttgttatc ggtgcgctga ttgtgttccg tattggctct
    11641 tttattccga tccctggtat tgatgccgct gtacttgcca aactgcttga gcaacagcga
    11701 ggcaccatca ttgagatgtt taacatgttc tctggtggtg ctctcagccg tgcttctatc
    11761 tttgctctgg ggatcatgcc gtatatttcg gcgtcgatca ttatccagct gctgacggtg
    11821 gttcacccaa cgttggcaga aattaagaaa gaaggggagt ctggtcgtcg taagatcagc
    11881 cagtacaccc gttacggtac tctggtgctg gcaatattcc agtcgatcgg tattgctacc
    11941 ggtctgccga atatgcctgg tatgcaaggc ctggtgatta acccgggctt tgcattctac
    12001 ttcaccgctg ttgtaagtct ggtcacagga acgatgttcc tgatgtggtt gggcgaacag
    12061 atcactgaac gaggtatcgg caacggtatt tcaatcatta tcttcgccgg tattgtcgcg
    12121 ggactcccgc cagccattgc ccatactatc gagcaagcgc gtcaaggcga cctgcacttc
    12181 ctcgtgttgc tgttggttgc agtattagta tttgcagtga cgttctttgt tgtgtttgtt
    12241 gagcgtggtc aacgccgcat tgtggtaaac tacgcgaaac gtcagcaagg tcgtcgtgtc
    12301 tatgctgcac agagcacaca tttaccgctg aaagtgaata tggcgggggt aatcccggca
    12361 atcttcgctt ccagtattat tctgttcccg gcgaccatcg cgtcatggtt cgggggcggt
    12421 actggttgga actggctgac aacaatttcg ctgtatttgc agcctgggca accgctttat
    12481 gtgttactct atgcgtctgc aatcatcttc ttctgtttct tctacacggc gttggttttc
    12541 aacccgcgtg aaacagcaga taacctgaag aagtccggtg catttgtacc aggaattcgt
    12601 ccgggagagc aaacggcgaa gtatatcgat aaagtaatga cccgcctgac cttggttggt
    12661 gcgctgtata ttacctttat ctgcctgatc ccggagttca tgcgtgatgc aatgaaagta
    12721 ccgttctact tcggtgggac ctcactgctt atcgttgttg tcgtgattat ggactttatg
    12781 gctcaagtgc aaactctgat gatgtccagt cagtatgagt ctgcattgaa gaaggcgaac
    12841 ctgaaaggct acggccgata attggtcgcc cgagaagtta cggagagtaa aaatgaaagt
    12901 tcgtgcttcc gtcaagaaat tatgccgtaa ctgcaaaatc gttaagcgtg atggtgtcat
    12961 ccgtgtgatt tgcagtgccg agccgaagca taaacagcgc caaggctgat tttttcgcat
    13021 atttttcttg caaagttggg ttgagctggc tagattagcc agccaatctt ttgtatgtct
    13081 gtacgtttcc atttgagtat cctgaaaacg ggcttttcag catggaacgt acatattaaa
    13141 tagtaggagt gcatagtggc ccgtatagca ggcattaaca ttcctgatca taagcatgcc
    13201 gtaatcgcat taacttcgat ttatggcgtc ggcaagaccc gttctaaagc catcctggct
    13261 gcagcgggta tcgctgaaga tgttaagatc agtgagctgt ctgaaggaca aatcgacacg
    13321 ctgcgtgacg aagttgccaa atttgtcgtt gaaggtgatc tgcgccgtga aatcagcatg
    13381 agcatcaagc gcctgatgga tcttggttgc tatcgcggtt tgcgtcatcg tcgtggtctc
    13441 ccggttcgcg gtcagcgtac caagaccaac gcacgtaccc gtaagggtcc gcgcaaaccg
    13501 atcaagaaat aatcggggtg attgaataat ggcaaaggca ccaattcgtg cacgtaaacg
    13561 tgtaagaaaa caagtctctg acggcgtggc tcatatccat gcttctttca acaacaccat
    13621 cgtgactatc actgatcgtc agggtaacgc gttgggttgg gcaacagccg gtggttccgg
    13681 tttccgtggt tctcgcaaat ccactccgtt tgcagctcag gttgcagcag agcgttgcgc
    13741 tgacgccgtg aaagaatacg gcatcaagaa tctggaagtt atggttaaag gtccgggtcc
    13801 aggccgcgaa tctactattc gtgctctgaa cgccgcaggt ttccgcatca ctaacattac
    13861 tgatgtgact ccgatccctc ataacggttg tcgtccgccg aaaaaacgtc gcgtataacg
    13921 cctcgttttc caggtttgtt ggagaaagaa aatggcaaga tatttgggtc ctaagctcaa
    13981 gctgagccgt cgtgagggca ccgacttatt ccttaagtct ggcgttcgcg cgatcgatac
    14041 caagtgtaaa attgaacaag ctcctggcca gcacggtgcg cgtaaaccgc gtctgtctga
    14101 ctatggtgtg cagttgcgtg aaaagcaaaa agttcgccgt atctatggtg tgctggagcg
    14161 tcagttccgt aactactaca aagaagcagc acgtctgaaa ggcaacaccg gtgaaaacct
    14221 gttggctctg ctggaaggtc gtctggacaa cgttgtatac cgtatgggct tcggtgccac
    14281 tcgtgcagaa gcacgtcagc tggttagcca taaagcaatt atggtaaacg gtcgtgttgt
    14341 taacatcgct tcttatcagg ttagtccgaa tgacgttgta agcattcgtg agaaagcgaa
    14401 gaagcagtct cgcgtgaaag ccgctctgga gctggctgag cagcgtgaaa agccaacttg
    14461 gctggaagtt gatgctggca agatggaagg tacgtttaag cgtaagccgg agcgttctga
    14521 tctgtctgcg gacattaacg aacacctgat cgtcgagctt tactccaagt aaagcttagt
    14581 accaaagaga ggacacaatg cagggttctg tgacagagtt tctaaaaccg cgcctggttg
    14641 atatcgagca agtgagttcg acgcacgcca aggtgaccct tgagccttta gagcgtggct
    14701 ttggccatac tctgggtaac gcactgcgcc gtattctgct ctcatcgatg ccgggttgcg
    14761 cggtgaccga ggttgagatt gatggtgtac tacatgagta cagcaccaaa gaaggcgttc
    14821 aggaagatat cctggaaatc ctgctcaacc tgaaagggct ggcggtgaga gttcagggca
    14881 aagatgaagt tattcttacc ttgaataaat ctggcattgg ccctgtgact gcagccgata
    14941 tcacccacga cggtgatgtt gaaatcgtca agccgcagca cgtgatctgc cacctgaccg
    15001 atgagaacgc gtctattagc atgcgtatca aagttcagcg cggtcgtggt tatgtgccgg
    15061 cttctacccg aattcattcg gaagaagatg agcgcccaat cggccgtctg ctggtcgacg
    15121 catgctacag ccctgtggag cgtattgcct acaatgttga agcagcgcgt gtagaacagc
    15181 gtaccgacct ggacaagctg gtcatcgaaa tggaaaccaa cggcacaatc gatcctgaag
    15241 aggcgattcg tcgtgcggca accattctgg ctgaacaact ggaagctttc gttgacttac
    15301 gtgatgtacg tcagcctgaa gtgaaagaag agaaaccaga gttcgatccg atcctgctgc
    15361 gccctgttga cgatctggaa ttgactgtcc gctctgctaa ctgccttaaa gcagaagcta
    15421 tccactatat cggtgatctg gtacagcgta ccgaggttga gctccttaaa acgcctaacc
    15481 ttggtaaaaa atctcttact gagattaaag acgtgctggc ttcccgtgga ctgtctctgg
    15541 gcatgcgctt ggaaaactgg ccaccggcaa gcatcgctga cgagtaaccg gatcacaggt
    15601 taaggtttta ctgagaagga taaggtcatg cgccatcgta agagtggtcg tcaactgaac
    15661 cgcaacagca gccatcgcca ggctatgttc cgcaatatgg caggttcact ggttcgtcat
    15721 gaaatcatca agacgactct gcctaaagcg aaagagctgc gccgcgtagt tgagccgctg
    15781 attactcttg ccaagactga tagcgttgct aatcgtcgtc tggcattcgc ccgtactcgt
    15841 gataacgaga tcgtggcaaa actgtttaac gaactgggcc cgcgtttcgc gagccgtgcc
    15901 ggtggttaca ctcgtattct gaagtgtggc ttccgtgcag gcgacaacgc gccgatggct
    15961 tacatcgagc tggttgatcg ttcagagaaa gcagaagctg ctgcagagta atctgaagca
    16021 atgtaaaaaa acccgccccg gcgggttttt ttatacccgt agtatcccca cttatctaca
    16081 atagctgtac tctttt
//
LOCUS       DAEBXN010000092        15935 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.092, whole genome shotgun sequence.
ACCESSION   DAEBXN010000092 DAEBXN010000000
VERSION     DAEBXN010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15935
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.092"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..756
                     /locus_tag="KJL99_003075"
     CDS             <1..756
                     /locus_tag="KJL99_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HBD4437717.1"
                     /translation="YTKILENDFEHPSIRRKIASLSNLGVDEWIPPVMAFMNRMARTE
                     DFNLDDFSQFITAFEKVYMHGWLKKQIKSQREMVCYSALVAINNDMPFDSVINQINQH
                     ADNSGFIAALDEDLYEPRPNQVNLIKAILLRLDMEQQDESVIKTYTGRITIEHILPQA
                     LVNEYWINRFQPQEHVYWLHKIGNLTLISGSKNSETQHYDFIKKKSIYEKLNSKSSFY
                     LTKDVCNSSEWGLAELKMRHEKMKTQLKKLWLV"
     gene            complement(825..1781)
                     /gene="yjiA"
                     /locus_tag="KJL99_003076"
     CDS             complement(825..1781)
                     /gene="yjiA"
                     /locus_tag="KJL99_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HBD4437718.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQLYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(1792..1995)
                     /locus_tag="KJL99_003077"
     CDS             complement(1792..1995)
                     /locus_tag="KJL99_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HBD4437719.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            complement(2045..4195)
                     /gene="btsT"
                     /locus_tag="KJL99_003078"
     CDS             complement(2045..4195)
                     /gene="btsT"
                     /locus_tag="KJL99_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HBD4437720.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            complement(4588..5100)
                     /locus_tag="KJL99_003079"
     CDS             complement(4588..5100)
                     /locus_tag="KJL99_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HBD4437721.1"
                     /translation="MQLDEQRLRFHDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            complement(5118..6676)
                     /gene="hpaB"
                     /locus_tag="KJL99_003080"
                     /pseudo
     CDS             complement(5118..6676)
                     /gene="hpaB"
                     /locus_tag="KJL99_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
     gene            complement(6903..7840)
                     /locus_tag="KJL99_003081"
                     /pseudo
     CDS             complement(6903..7840)
                     /locus_tag="KJL99_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601398.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            complement(7867..8757)
                     /gene="hpaA"
                     /locus_tag="KJL99_003082"
     CDS             complement(7867..8757)
                     /gene="hpaA"
                     /locus_tag="KJL99_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HBD4437722.1"
                     /translation="MSSEAIFEHGYENIDLVREYDARYAGEDVHYETFARLANFFGRD
                     MRPHWHDRYFQLHYLVTGRITLQLDEHFYSVRAPLFVLTPPSVPHTFFTDPDSDGHVL
                     TVRQELIWPLVEKLWPGSGDAAMLQGICLSLEGMEHTLAALNHYWPIISLEFQQNKQG
                     REILLQSLAQSIFTILLREAPPNDISTCSVRGEMCLFQKFNRLIDENYRQHLMVPEYA
                     TMLGMSESRLTELCRRFANQSPKRLIFERVSREARRLLLYSEQSINQIALDLGYKDPA
                     YFARFFNRMMGCSPTQFRGR"
     gene            complement(8767..10122)
                     /gene="hpaX"
                     /locus_tag="KJL99_003083"
     CDS             complement(8767..10122)
                     /gene="hpaX"
                     /locus_tag="KJL99_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HBD4437723.1"
                     /translation="MTTSTLQYKDNKTVEIEHRVINKLFRRLIIFLFILFVFSFLDRI
                     NIGFAGLTMGKDLGLTSTMFGLAATLFYVTYVMCGIPSNMMLAKIGARRWIAGIMVVW
                     GIASTCTMFATSPETLYLLRMLVGIAEAGFLPGILVYLTWWFPAYHRARANALFMIAM
                     PVTMMLGLILSGYILALDGLWNLKGWQWLFLLEGLPSVILGVVTWFYLNDTPDQATWL
                     DENEKQALKTMILREQESAFAHASVPRSTLREVLTPAIMLYTLAYFCLTNTLSAINIW
                     TPQILQSFNTGSSNIMIGLLAAIPQFCTIFGMIWWSRRSDRLKERKKHTILPYLFAAA
                     GWLLASATDHSLIQLSSIIMASMGSFTAMTIFWTTPDQVISLQSRAVAMAVINAVGNV
                     GSAVSPLLIGILKDATGSFSSGLWFVAGLLVLGALVMTRIPMGSREHSLSSELIAPKP
                     Q"
     gene            complement(10144..10947)
                     /gene="hpaI"
                     /locus_tag="KJL99_003084"
     CDS             complement(10144..10947)
                     /gene="hpaI"
                     /locus_tag="KJL99_003084"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HBD4437724.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGPGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     KAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGNSSSISMAKQNNNSVY"
     gene            complement(10958..11761)
                     /gene="hpaH"
                     /locus_tag="KJL99_003085"
     CDS             complement(10958..11761)
                     /gene="hpaH"
                     /locus_tag="KJL99_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HBD4437725.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAESRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLETGQIILGGSFTLPVPARKGDTFHVDYGNMGSISCRFV"
     gene            complement(11829..12209)
                     /locus_tag="KJL99_003086"
     CDS             complement(11829..12209)
                     /locus_tag="KJL99_003086"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HBD4437726.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            complement(12219..13070)
                     /gene="hpaD"
                     /locus_tag="KJL99_003087"
     CDS             complement(12219..13070)
                     /gene="hpaD"
                     /locus_tag="KJL99_003087"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HBD4437727.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     AEEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            complement(13072..14538)
                     /gene="hpaE"
                     /locus_tag="KJL99_003088"
     CDS             complement(13072..14538)
                     /gene="hpaE"
                     /locus_tag="KJL99_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HBD4437728.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWADLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALASPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVRALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            complement(14535..15824)
                     /locus_tag="KJL99_003089"
     CDS             complement(14535..15824)
                     /locus_tag="KJL99_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HBD4437729.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALDNDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPSDAILLGTPQARVEIQPSDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
BASE COUNT         3760 a         4150 c         4167 g         3858 t
ORIGIN      
        1 tatactaaga ttttagagaa tgattttgaa cacccttcaa tacgacgaaa aatagcatcg
       61 ctaagtaatt taggggtaga tgagtggatt cctccggtta tggcattcat gaatcgaatg
      121 gctcgtacag aagattttaa tttagatgat ttttctcaat ttattaccgc ctttgagaag
      181 gtatatatgc atgggtggtt aaaaaaacaa attaaaagcc aaagagaaat ggtttgttat
      241 tctgctctgg tagcaataaa taatgatatg ccttttgatt ctgtgataaa tcagataaac
      301 caacatgcag ataattcagg ttttatagct gctctggatg aagatttata tgagcctcgt
      361 ccgaatcaag tgaacttaat aaaagcaatt ttgcttcgct tagatatgga gcaacaagat
      421 gagagcgtga ttaaaaccta tacggggaga atcacaattg agcatattct tcctcaagca
      481 ttagttaatg aatactggat taatagattc caacctcaag agcatgtcta ctggctgcat
      541 aaaataggta atttaacact cataagtggt tccaaaaatt ctgaaacaca acactatgat
      601 tttatcaaaa agaaatctat ttatgagaaa cttaatagta aaagctcttt ttatttaaca
      661 aaagatgttt gtaattcttc agaatgggga ttggctgaac tgaaaatgag acatgaaaaa
      721 atgaaaacgc aattgaagaa gctttggttg gtctgacata tctaaagagt tccactcgta
      781 ataatgttgc ggtggaactc tcaaaaagtt gatctttccc gaacttactt cctcaatccc
      841 gcaaacgccg cccgaatctc ttcctccggc aactgaatcc cgataaacac cattgtgcta
      901 tgcggttttt catcgcccca cggcctgtcc cagtcggcgc tgtagaggcg ctggacgccc
      961 tggaacagca ggcggttagg ttcgccgtca atccacagca tccctttgta acgcagcagt
     1021 ttatccgccg attccagcag caggttttcc atcacgcggg aaacttcgct gatatctacc
     1081 gggtaatcca gttccaccac aattgacgaa atatcgtttt gtttatccgc gataaagtgg
     1141 aaacgcggtt tggtgctgac gacgttctct tccagcataa aaccgttggt gttgaacagc
     1201 aggcccaggt cgatgtcgcc gtgggtgacg gtgtagaccg gtgcgcgggc gttgatgcgc
     1261 gccaggcgtt cacgcagttt ctctgcttcg cctgcgacgt cggttttagt cagcagaata
     1321 cggtcggcgt agcccacctg ggactgggcg atggtgaact ggttcatctg ctcatcggca
     1381 tgtaccgcat ccaccagcgc aatcacgccg tccagcaggt agagctggca taaaatctca
     1441 tgggagaaaa aggtctgaat aatcgggccg ggatcggcca tgccggtgca ttcaatcacc
     1501 agacgatcga actgaatatt gcccttgtcg agattatcca gcaggtcgag tagtgcgtcc
     1561 tccagctcgt tggagcgcga acagcagatg cagccgttgg tcagcgtttt gatctgcgtg
     1621 gcgcggtcgc caatcagttg gtcatcaacg gagacttcgc cgaattcgtt ttcaatcacg
     1681 gcaatcttgt agccgtgttg ttcgttaaga atatggcgca gcagggtagt tttgcccgcg
     1741 cctaaaaaac cggtgagtag ggtaactgca atcgggttca tgctctctcc attaacaaca
     1801 gcgcataccg cctttaccat cgccgccgta gcgcgcgttc tggcgttcgc ggaagaattc
     1861 ttcatagctc atgtacggct tgtcgggatg gttggtcttc atatgctcga cgtagttgtc
     1921 gtagtctgga ataccaatca acatcttcgc cgcctggccg agatattttt ttgcctgtcc
     1981 taagttacca aacatagttc actctgataa gaacaaagcc ccgccgaagc ggggctaaac
     2041 acggttagtg gtgcgaagag atcttcacgc cgccttccgg gattggaacg tacggtgttt
     2101 ctttgtcagt acgtttgtcg ctgttacgca ccgcaagcca ggttttgaaa ccgtagaaga
     2161 tgatgctgta caccacaatc aggaacagaa tactcaggcc tgcgttggtg tagttgttca
     2221 caacgatgtg gttcatgttg gcaatctgct gcgccgtcag gtcagtaccg ttagcaatct
     2281 tctctttgta ctggcttgcc atgtagaaga agccttccat ctgcgggttg gtgctgaaca
     2341 gtttcagacc cagcgcccag gtggtgcaga taagcagcca tacagccgga acaacagtta
     2401 cccagatgta ttgggtgcgc ttcatcttaa tcagcacaac ggtgcccagt accagcgcta
     2461 cggctgccag catctggttg gagataccga acagcggcca caggctctta acgccgccca
     2521 gcggatcgac cacgccctga tacagcaggt agccccacag acccacacag cccgcagtac
     2581 cgatgatacc ggcaaccaga gagtcggttt ttttcaggaa cgggatgaag ttacccagca
     2641 ggtcttgcag cataaagcgg ccagaacggg tacccgcatc cagcgcggtc aggatgaaca
     2701 gggcttcgaa cagaataccg aagtgatacc agaagcccat gtcagccatc ggcagcactt
     2761 tgtggaacac gtgagcgata cctaccgcca gcgtcggcgc accacctgca cggttcagga
     2821 cagaaggctc accaatgtct ttcgcggttt gcaggatctg ctctggcgaa atcacgaagc
     2881 cccaggagct gacggtcgct gccgcgtgtg cggtaacgtc tttcagctgc gccatgatga
     2941 tcggcgcgtt ctcgccaccc atttcatgca ggttaggcat ggtgatgcca aggccagcag
     3001 gcggggtgtt catcgcgaag taaagacccg gttcgatgat ggacgctgca accagcgcca
     3061 taatcgccac gaaggactcc atcagcattg cgccgtagcc aatgaaacgc gcgtcggttt
     3121 cgttagccag cagtttcggc gtcgtaccgg aagagatcag cgcgtggaag ccagataccg
     3181 caccacaggc gatggtgatg aacaggaacg ggaacagagc gcctttccac agcgggccag
     3241 taccgtcaat gtactgggtc atcgcaggca ttttcagttc cgggttcagc accacgatac
     3301 ccagcgccag gccgacgata acgccgattt tcaggaaggt tgccagatag tcgcgcggtg
     3361 cgaggatcag ccacaccggc agcagtgcgg aaacaaacgc atagccaatc agcgcgaagg
     3421 taatggtggt gtctttaaag gtcagtgccg gaccccagta cggatcgtga gcaatcacgc
     3481 caccgaagta gatagaggca accagcagca cgataccaat aacagagact tcacccacac
     3541 gccccggacg gataaagcgc atgtagatac ccataaacag cgcaatcggt acggttgagc
     3601 aaacggtgaa gacaccccac ggactttcgg ccagggcttt aaccacaatc agcgccagaa
     3661 cggcgaggat gatgatcatg attaagaaac agccaaacag cgcgatagtc cccggtactg
     3721 gccccatctc ttctttgatc atctcaccga gagatgcgcc gttacggcga gaggagataa
     3781 acagcaccat aaagtcctgt accgcaccgg ccagcactac ccccgccagc agccacagcg
     3841 tgccaggcag gtagcccatc tgcgcggcga gaaccggtcc caccagcgga ccagcaccgg
     3901 cgatagcggc gaagtggtga ccaaacaaca cgtaacggtt ggtcggaacg tagttcagac
     3961 cgtcgttgtt aataaccgca ggcgtcgcgc gcgtggggtc gagtttcatc accttctggg
     4021 cgatgtacag actgtagtag cgatacgcca ccagatacac cgacacagag gcgaccacga
     4081 tccacagggc gctgacgtgc tccccccgac gtaatgcaac taccgcgagg cagaatgcac
     4141 cgatgattcc gagaatcacc cagggtatgt gcttgaatag ttttttcgta tccatagtaa
     4201 aacctggcat tttactcgtc gtcttttggc ttacaggtgc ggcagctgcg ttcgttcacc
     4261 tctgacactt acttttgtaa gctcccgggg attcactcac ttgctgcctt cctgcaaacc
     4321 aaaatccata gagtaatttg ttaatcatag gccgaagccg ttctaggttt gcgttgcgtt
     4381 tgaggaggta aattgaccgc tatcctgcag tgatgttgcc agagatacgt gcgcgtaaag
     4441 taaggtaaat aactgagtgg ttattttagg gatgtaagcg gtcaggaatg gcggtgagcg
     4501 gttgggcaag aattattcat cgggtagata tgtgattgag atcacaataa aaatgcccct
     4561 tcgacgaatg cgtgaagggg ctggagctta aatcgcagct tccatttcca gcatcaccgg
     4621 atggaaacgg cgtttaaagt aaataagtcc gtgaccttct gcactgagga tgatgttttt
     4681 aatctccacc agatacacca gatgtgtgcc aatcgcctgc acatcgcgga tctctccttc
     4741 aagactggcc agcgaacctt ttagcaccgg ctgcgccagc ggaccttttt gccagcatga
     4801 gaggctaaaa cgctcttcca tcgccatgcc tgtcatgccc gcgaagtggc gcgccatcag
     4861 ttcctgctca tggttgagga cgttgacgca caacttgccg ttgccctgaa aaaccgggtt
     4921 catcgcactg ttggcgttaa tgcacaccat cagcgacggt ggtgtatccg tgaccgagca
     4981 gacagccgtt gccgtaatcc cgcattgtcc ggcgtcgccc tcggtggtga taatatttac
     5041 cgctgccgac aggctggcca tcgcgtcatg aaagcgcagg cgttgttcat ctaattgcat
     5101 cttaacctcc tgctgcgtta tttcagcagc ttatccagca tgttgatatc gtcgttgttg
     5161 tgcaggtgcg gcacagtcca gccgttctgg tcgtattccg acaggcagcg atcaaccatc
     5221 gccatcatct tgtccatatt gccggagctt tgtgcctggc gcagacattg caggcgaatc
     5281 tcatcctggc taccggagta gttgatttca tacagctcgt gacgaccacc aaattcgctg
     5341 ccaatggcgt cccacatcag tttgaggatc ttgatgcgct ggacgtgatc cataccgttc
     5401 gaaccgcgca catacttcgc cagatactgg tcgatctgcg gattgttcag gtcacgggca
     5461 ctggaaggga ggtagatcag gccactggta acgttgcgtt ctataatgtt tttgatcttc
     5521 gcgtaggcca ttggtgccag tacgcgatag gtttgcagtg cggcatgatc cggtaaataa
     5581 gccccgttga cccacggcgt cgcttctgaa cacatcgagt cactcaatgc ccagaaggtg
     5641 ttgcgccacg ccaccacttc accgagatcg gcctgcacac cacggaactc cagggtgccg
     5701 gtacattcga gtgatttttt cagcagtgcc gtaatgaagt cgagtttcac tgccaggcgc
     5761 acacaggctt gcagcggata catacgggcg aaaccgcctt ccatcgtcca gcgacggcag
     5821 cgatcaaaat cgcggtagat cagcacgttt tcccatggga tcagtacgtt atccatcacc
     5881 agaatcgcat cgttctcatc gaagcggctg gagagcgggt agtcgtatgg cgagccggta
     5941 gcacccgcga ccatctcata agaggcgcgg gagattaatt tcacgccatc ggcatccatt
     6001 ggcgcaacga acatcagtgc gaagtccggg ttttcgccca tcacttgtgc cgagccgaag
     6061 ccaatcatgt tgtagtgagt cagcgccgag ttggtggcaa ccactttcgc accgctgacg
     6121 ataatcccgg cgtcagtctc tttttccagc ttgatgtaaa cgtctttcac tttatcggtc
     6181 ggcaaatgac gatcggtggg ttaacaatcg cgtggttaaa gtagaggcca gtttcctgaa
     6241 tacgggtata ccagttacgg gcgttctgct cgaactgacc gtaaaagccc ggattcgcgc
     6301 ccagtgcgca accgaaagcg gctttgtagt ctggggtacg gcccatccag ccatagctca
     6361 ggcgtgacca ttcagcgatg gcgtcgcgtt gctggcgcag gtcgtcggca cttttcgcca
     6421 cgcggaagaa tttatgggta tagccgccgc tgccggtgtc ggtgttccaa cacagagagt
     6481 cctgcatctc cggtttgtgc agcgcgtcgt acagttgggc aacagacgct gccgcattac
     6541 gaaatgccgg atgagtggtg acgtctttca ctcgctcgcc atagatatag atctcgcgac
     6601 catcctgcag gcttttcaga tactcttccc cggtgaaagg acgttgggta ctggcgcgga
     6661 aatcttctgg tttcatgtcg acctctacag tattaatcgg aatacgattg ttaattttgt
     6721 gtgttgtttt tgttatttaa ttgaagcgtt gaacgcggtt gtcgggaaga gacgtttcag
     6781 gcgaacgttg gtacttttcg acagcaaggt ggagttaaat agtaaactgc gatcgtgatc
     6841 aacttacagt aaggcaaatc ataatattcc ccgctaacaa gggctaacgg ggacggaatt
     6901 ttttatgggg cgaatggaat ggtaatcgtc atattttcat gaccaacgac gataggacct
     6961 gaatagtttt ctgtgcttct tgcttccatg tttcatccgg aacggtgatt ggtaccaggt
     7021 ggctaaggat gagttttttc acttttgctt cattggcaat tttgcctaca tcctcaatag
     7081 tcgtatggtg agaagcaatg gcttttgcca gtgttttacc attgccaata ctgtctgcca
     7141 gcttagcgac ggagggctca tgtacggcct catgcacaag tatatcagcg ccttctgaga
     7201 aagttgccag tgggggaaag tacgaggtat ctcctgagaa gacaattcgc ttgccttgtg
     7261 tgtcgaagcg ataagcaaaa gcctcaccgt cagggaaagg agaatgaggg acctttaatg
     7321 cagtcacggt cacggtatca tctttataaa ctacgccttc accaacatct tgagctgtaa
     7381 cgagtttgcg aggatcgggt tttccttctt cttccatacg cagtgtgatg tcccgatcga
     7441 agacctgcca actggcagcg gtcatggctc gggtgcctgg cggaccgaat acatgaatag
     7501 ttttgtcctg cagaccgcta gcccatgcat tcattaagac tgatggatag tccagaatat
     7561 gatcactgtg taaatgggtg agtaaaatat tatcaatctg gcgcagtgga atattatttt
     7621 ttaccagttg taagctggcc ccgtaaccag catcaattaa ccaggtttta ttgccaataa
     7681 taagtgccgt tgccatcggt aaacggtcag cttttaataa agaagggcca cctttcgttc
     7741 caagtagtac aattttcatt tattgcggtt cggaggcgta ggtgaatgcc actggcgcaa
     7801 tcaggcacgc cacaaatagt gagcgtaaag atattttcat gtcgagtctc tcaggttgat
     7861 aaatggtcag cgaccacgga attgtgttgg tgagcaaccc atcattcggt tgaaaaaacg
     7921 ggcgaaatag gcgggatctt tgtagccaag atcaagggct atctggttga tgctttgctc
     7981 gctatagagc agaagccgtc tggcttcccg gctcactcgt tcgaaaatca atctcttcgg
     8041 cgattgattg gcaaaacgtc ggcacagctc ggtaagccgt gattcactca taccgagcat
     8101 tgttgcatat tcagggacca ttaaatgttg gcgataattt tcgtcaatca gccgattaaa
     8161 cttctggaat aaacacatct cgccgcgcac gctgcatgtt gaaatatcat ttggtggtgc
     8221 ttcacgcagt aggattgtaa aaatactttg cgccagtgat tgcaatagaa tttcccgtcc
     8281 ttgcttattt tgttgaaact ccagggatat tatcggccaa taatgattta atgccgccag
     8341 tgtatgttcc attccctcca gtgacagaca gataccttgt agcattgcgg catctccact
     8401 accaggccag agcttttcta ccaggggcca aattaactcc tgacgtacgg tcagaacatg
     8461 cccatcgcta tcaggatcag taaagaaagt atgtggcacc gatggtggtg ttagtacaaa
     8521 aagcggtgcg cgcacagaat agaagtgctc atccagttgt aaagtaatgc gtccagtgac
     8581 cagataatgg agttgaaaat atctatcgtg ccagtgaggg cgcatatcgc gtccaaagaa
     8641 attcgctaac cgggcaaatg tttcataatg cacatcttca ccagcgtagc gggcatcata
     8701 ttcccggacc aaatcaatat tttcataacc atgttcaaag atagcttcac tggacatcat
     8761 gactccttat tgcggttttg gtgcgataag ttcagatgaa agagagtgtt cccgactccc
     8821 catagggatt cgggtcatga ctaacgcgcc cagtaccagc aatcctgcta caaaccacaa
     8881 acctgagcta aaactgccgg ttgcatcttt caaaataccg ataagtaatg gactgactgc
     8941 tgaacctaca ttgccgacag cattgatgac agccatcgca acggcacgcg attgcaagct
     9001 tataacttga tccggtgttg tccaaaagat agtcatcgcg gtaaatgatc ccattgaggc
     9061 cataatgatg ctgctcagtt gaatgaggct gtgatctgtc gcagaagcga gtaaccatcc
     9121 ggcagcggca aacaggtagg gaagaatcgt atgctttttg cgttctttaa gacggtcgga
     9181 acgacgactc caccagatca tgccaaaaat tgtgcagaat tgggggatgg ccgccagcaa
     9241 accaatcatg atgttgctac tgccagtatt gaaactctgt aggatttgcg gcgtccagat
     9301 attaatcgca ctcagagtat tagtcaggca aaaataagca agtgtatata gcatgatggc
     9361 tggggtcagg acttcacgta aggttgagcg tggaacagat gcatgggcaa atgcactctc
     9421 ttgttcacgg agaatcatcg tttttaaagc ctgtttttcg ttttcatcaa gccaggtcgc
     9481 ctggtctggt gtgtcgttga gataaaacca ggtgacgaca ccaagaatga ccgatggcag
     9541 accttccagc aaaaagagcc attgccagcc ttttagattc cacagtccat ccaaggccag
     9601 aatgtagcct gaaaggatca atcccagcat catagtgact ggcatcgcta tcataaataa
     9661 ggcattggct ctggcccgat gatatgccgg gaaccaccag gtcagataaa ccaaaatacc
     9721 aggtaaaaaa cccgcctcag caataccaac cagcatacgc agtagataga gtgtttcggg
     9781 gcttgttgca aacattgtgc aggtggaagc aattccccag accaccatga tcccagcgat
     9841 ccatcgacga gcgccaattt tcgctaacat catattactg gggatcccgc acatgacgta
     9901 cgtgacataa aagagtgttg cggcgaggcc aaacattgtg gacgtcaggc caagatcctt
     9961 gcccattgtc agtccggcaa atcctatatt gatgcgatca agaaacgaaa aaacaaacag
    10021 aataaaaaga aaaatgatta ggcgacgaaa taatttatta atgacacgat gttcaatttc
    10081 aacagtttta ttatctttat attgcagggt agaggtcgtc atgatgcgct ccacatcgta
    10141 cgtttagtag acagagttgt tgttttgctt cgccatcgaa atagaagaac tattaccaaa
    10201 ccgtgctgcc agcgcttcgg cggcgcgggc gagcagggtg gtgtcaacgc cgacggcgac
    10261 aaacagcgcg cccagttcca gatagcgttt tgccagttgc tcattggcga tcaggatccc
    10321 cggcgctttg cccgcttcgc ggatctgcac gatcgcctgc tcaatggcgg cctgtacttc
    10381 cgggtgctgc ggattaccgg cataacccat atcggcgctc agatccgccg ggccgataaa
    10441 cacgccgtcg accccttcca cgtccagaat ctgcggtaag ttcttcattg cctcacgcgt
    10501 ttcgatctgc accagcacgc acatttgatc gttggctttt tgcaggtaat caggaatgcg
    10561 attccagcgc gaggcgcgag ccagcgcact gcccacaccg cgaataccgg cggggggata
    10621 acgggtggcg cgtaccgctt tacgggcttc gtcggcgttt tgtaccatcg gcaccagtaa
    10681 ggtttgtgtg cctacgtcca gcagttgttt gatttgcacc ggatcgttcc acgatgggcg
    10741 taccactggc tggctggggt agggcgcaat cgcctgtagt tgggtgagca cggtttgcac
    10801 gttattcggc gcgtgctcac cgtcgatcaa taaccagtcg aatcctggtc cggccagtaa
    10861 ctccgcgctg tagctgctgc tcagccccag ccataatcca atctgcggac ggcctgcttt
    10921 cagcgccgct ttaaaactgt tttccatcgt tctctcctta aacaaagcgg cagctaatgg
    10981 agcccatgtt gccgtaatcg acgtggaagg tgtcgccctt acgcgccgga accgggaggg
    11041 tgaacgaacc accgagaatg atttgcccgg tttccagttg tacgtcatac ggtgcgagtt
    11101 tgttcgccag ccaggccacg ccgtttgccg gatgattcag cacaccagcg gcgacgccgg
    11161 tttcttcaat cacgccattg cgatacatca gggcggagat ccaacgcaga tccagttcgt
    11221 ctggtttgat cggacgacct ccgaggatca ctccggcgtt ggcggcgtta tcggaaatgg
    11281 tgtcgaatac tttgcgcggg cgctgggttt ccggatcgat gttgtggcag cgagcgtcga
    11341 tcagctccag cgccgggatc acatagtccg tggcgttgta aacgtcgaac agcgtgcagt
    11401 ttggcccacg cagcggtttt gccagcacaa aagccagctc cacttcaatg cgcggcacga
    11461 taaagcgatc ggtcgggata tcgctgccat cgtggaagaa catgtcgtcc agcagtgcac
    11521 cgtaatccgg ttcgctgatc tgcgagcttg cctgcatcgc tttcgaggtc agaccgattt
    11581 tgtggccttt cagcgtgcga ctttcggcga ttttcaggcg aacccattcg cgctgcacgg
    11641 cgtaggcgtc ttcgatggtg atctccgggt aatccagcga gatcgcgcgg atctgttcgc
    11701 gctgtttttc tgcctgatcc agacgctggg cgatcagggt gtgggtgtgt ttatcgaaca
    11761 tagcgatatc ctgtggcatt gccggatgcg gcagcgcctt atcatccggc ctggaagctg
    11821 gtgcaaattt atttaaacaa cgcgtgtacg ttgttctgtt tgaaattcaa cgtcgggtgc
    11881 agctctgcaa tctcaaatga aagcgctagg taacggctct ccatcagtga ggcgaagtgc
    11941 gttttaatca gatcaaacag catttcgccc acttcctgac ggctttccag actgcgtccg
    12001 ctgccgattt ttagcgtcat atgcacaaag gcgtaatcgt gcttgccatc agccatttgc
    12061 caggtatcga tccagtgcgc ccggctacgt attcccccaa gcggaaaaat ccccgttgcc
    12121 gccagtgcag tgttaacact ggcaaacagc tcaggtaagc gggcttcttc acgaatattt
    12181 tcagtacatt ccacgataaa gtgcggcata aaacgtcctt acgcgggcag cgggaaaaca
    12241 gcgttaacct gaccggtgcc agagcttggg aacagctcgg taataaactc caccttgccg
    12301 tcgtatttat cccagccgag catccccagc agcatcaccg tgtcgtgcat attgccttcg
    12361 ccgtagcagt agtcggcgta ctccggcagc atattgcaaa actctttgaa ctggccttcg
    12421 cgccacagct tcaccacacg ctcgtccatc tggcggtcga actcgcgggt gtagctgttc
    12481 atcccttctt ccgcacgctg atcgtcaata aagcggtgcg ataacgaacc gctggcaagg
    12541 accgccacgg tgccgtcgta ctgttcgatc gctttcagaa tcgcttcgcc cagcttgcgg
    12601 ctgtcggcaa aatcgtgaac cgtgcagaaa gccgaaatgg agaccacttt gaagtgcttg
    12661 tcttcattca tgtagcgcat cggtaccagc gtgccgtatt ccagtttcag gctgggaata
    12721 ttgtgcgctt ttgcccgcac gccgagcttt aaggcttcct cggcaattag ctgccccaac
    12781 tccgggttgc cctcgtagtt gtaggtcatg tcgcgaataa aatgcggcag ctcgttgctg
    12841 gtgtagacgc cttcaaaatg gtctgcacag ttgatgtgat aagcactgtt gaccagccag
    12901 tgagtatcga aaacgataat ggtatcgacg cccatttccc ggcaacgctt gctgatctct
    12961 ttatgcccgt cgatcgcacc ctggcgacaa ccgtggtttt ttcctggtag ttcagagaga
    13021 tacatcgacg gtacgtgcgt gatcttggcg gctaacgcta acttacccat atcagactcc
    13081 ccatttcgga attggatggt cgcccatgga aatgcagacg ttcttcattt ccgcgaacac
    13141 ttcgaagctg tactcaccgc cttcacgccc ggtgccggag gcttttacgc cgccaaatgg
    13201 ctggcgcagg tcgcgcacgt tctgggtgtt gacgaacacc atgcctgctt caatgccacg
    13261 cgccaggcgt aacactttgc tgacatcctg tgtccagatg tacgacgcga ggccgtactc
    13321 cacgtcgttt gccaggcgta agccttcggc ttcgtcttta aacggcagca ggcaggcgac
    13381 cggcccgaaa atctcttcct gggcaacgcg catacggtta tctacgtccg ccagcaccgt
    13441 tgggcgcagg aagttgccgc ctttcaggtg cgcaggcagg tcggacggtt tatccgggcc
    13501 gcccgccaac agggttgcgc cttcttcaat gccgagacgg atatagccgg agactttttc
    13561 ccagtgttgc tgactgataa gcgccctaac ctgggtattc ggatcgttcg gatcgcccac
    13621 gcgcagacgg ttggcgcgtt cggcaaagcg tttaacgaat tctggataga tgctctgctg
    13681 aataaagatg cgcgaaccgg cggtgcagcg ttcgccgttg attgagaaga tggtgaacag
    13741 ggcggcgtcc agcgcacgct caatatcggc atcttcaaaa atcagcaccg gtgatttacc
    13801 gcccagctcc atggagtatt ttttcagccc ggcgtttttc atgatgttgc gcccggtggc
    13861 ggtaccgccg gtgaacgaca cggcacgcac gtcatgatga cggaccagcg catcgcctgc
    13921 ggttgcgccg tagccctgta ccacgtttag cacgcccgcc ggaataccag cttccagcgc
    13981 cagctcaccc aggcggtcgg cggtcagcgg ggagagttcc gacatcttca gcaccgcggt
    14041 attgcccagc gccagacacg gcgcgacctt ccaggtggcg gtcataaacg gcacgttcca
    14101 cggcgacgcc agcgcacaaa cgcctaccgg ttgcaccagc gtgtagttga gcatcttgtc
    14161 gtcaaccgga taggtcttgc cgttcatctg ctggcagact tccgcgaaaa attcaaagtt
    14221 gtgagaagcg cgtgggatca acacattttt ggtctgatgg atcggcaggc ccgtgtccgc
    14281 ggtttccatc gcggcgatct caggcacgtt ctggtcgatc agatcgccca gacggcgcat
    14341 caggcgcgca cgctctttca tcggcagatc agcccatttc gggaacgcct ctttcgctgc
    14401 cgctaccgcc tgattgatct ccgcttcacc gccagaggcc acatccgcca gcacttcacc
    14461 cgttgccgga ttggtagtct ggaagtagtc gttacctgca acatttttgc cgttgatcca
    14521 atgatttact tttttcattt cgctgtttcc tcactcacaa ttcggttcac caggcggccc
    14581 acgccttcta cttccaccac tacttcatcg ccaggcacca cgtcagataa gccttttggt
    14641 gtgccggtgg cgatcatgtc gcccggattc agggtcataa attcgcttaa gtaggcgatc
    14701 aggaagggca cgctgaagat cagatcggcg gtggtgcctt gctggcgtaa ctcgccgttg
    14761 acgaaggtgc gaagggtcag attatgcggg tccgggatcg cctctttcgg cacgatggtt
    14821 gaaagcatcg gcgtcagtcc gtcgcggctt ttgacccgca ggttagggcg gtagtagttt
    14881 tccagatagt cgcgaatggc gtagtcgtta cacacggtgt agcccgcgac ataatccatg
    14941 gcatcggctt cgctgacgtt acgcgcctgc ttgccaataa ctaccaccag ctccgcttcg
    15001 tagtgcatgt attcaatatt gttcgggcgc acggaggtct ggttgtcgcc agtgagggta
    15061 ttcggcgctt tcaggaacac cagcggctct tccggtggct taaattccag ttcgctggcg
    15121 tggtcggcgt agttcaggcc gagggcaaac agcgtaccgt gcgggtgtgg cagcgttggg
    15181 aagctcttgc gcgtggtcac ttcacgttcg tccactaccg gattttccag cggcgggaaa
    15241 ccttctgcga gaacacgcac gcgatcgctt ggctgtattt ccacgcgcgc ctgtggcgtg
    15301 ccgagcagaa tagcatcgct tggattcagc gtggcaaatt cgctcagggc gctcagcagc
    15361 tgtgcggcgt tacgttgtaa atcggcggta ttccagtgat cggcaggacg cccgttgatc
    15421 tcggtataga tagtcagatt atcgacattg ctgagagcca cggtttcgcc aatggggcag
    15481 aatccatcac gacattttgc tttgattgcc ggacggtaaa agctctcttc cggcaggctg
    15541 acgtcgttat ccagcgcata tccggcgatg tactctgccg catcttcttc acgtactttc
    15601 gtcgccgttt tccccacaat cagcgcaaca gtcgcaccgc tcagtacttt ttcaccctgt
    15661 ggaaagggaa tcggttcacc gcaaccaatc accgtattgc gcggtttaat aaaccagacc
    15721 gcagttttag gcggggcttt gtagggagat tgctggaacg cttcctgcca ggcatcaagt
    15781 tggctgcgat ggttcaacgc tacggcgaag atagtgcctt tcattaccac tcctcggatt
    15841 cgatcatctt aatcattaat atgttaatga ttacttttat tcccgattgc gtagtttggc
    15901 aaaagtaagt gatataaatg tgatctaatt aacaa
//
LOCUS       DAEBXN010000093        15852 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.093, whole genome shotgun sequence.
ACCESSION   DAEBXN010000093 DAEBXN010000000
VERSION     DAEBXN010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15852
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.093"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            45..287
                     /locus_tag="KJL99_003090"
     CDS             45..287
                     /locus_tag="KJL99_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4437730.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAESAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            401..1039
                     /locus_tag="KJL99_003091"
     CDS             401..1039
                     /locus_tag="KJL99_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HBD4437731.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1036..1773
                     /locus_tag="KJL99_003092"
     CDS             1036..1773
                     /locus_tag="KJL99_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HBD4437732.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            1773..3869
                     /locus_tag="KJL99_003093"
     CDS             1773..3869
                     /locus_tag="KJL99_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HBD4437733.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAHKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(3916..4194)
                     /locus_tag="KJL99_003094"
     CDS             complement(3916..4194)
                     /locus_tag="KJL99_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4437734.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4195..4338
                     /locus_tag="KJL99_003095"
     CDS             4195..4338
                     /locus_tag="KJL99_003095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4437735.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            4464..4874
                     /gene="psiE"
                     /locus_tag="KJL99_003096"
     CDS             4464..4874
                     /gene="psiE"
                     /locus_tag="KJL99_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HBD4437736.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(4918..6393)
                     /gene="xylE"
                     /locus_tag="KJL99_003097"
     CDS             complement(4918..6393)
                     /gene="xylE"
                     /locus_tag="KJL99_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HBD4437737.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKISAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGRRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQTESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQASGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(6764..7654)
                     /gene="malG"
                     /locus_tag="KJL99_003098"
     CDS             complement(6764..7654)
                     /gene="malG"
                     /locus_tag="KJL99_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HBD4437738.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(7669..9213)
                     /gene="malF"
                     /locus_tag="KJL99_003099"
     CDS             complement(7669..9213)
                     /gene="malF"
                     /locus_tag="KJL99_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HBD4437739.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(9367..10557)
                     /gene="malE"
                     /locus_tag="KJL99_003100"
     CDS             complement(9367..10557)
                     /gene="malE"
                     /locus_tag="KJL99_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HBD4437740.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSTGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            10922..12037
                     /gene="malK"
                     /locus_tag="KJL99_003101"
     CDS             10922..12037
                     /gene="malK"
                     /locus_tag="KJL99_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HBD4437741.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIYIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            12109..13449
                     /gene="lamB"
                     /locus_tag="KJL99_003102"
     CDS             12109..13449
                     /gene="lamB"
                     /locus_tag="KJL99_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HBD4437742.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            13601..14521
                     /gene="malM"
                     /locus_tag="KJL99_003103"
     CDS             13601..14521
                     /gene="malM"
                     /locus_tag="KJL99_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HBD4437743.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQELGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            14750..15852
                     /locus_tag="KJL99_003104"
                     /pseudo
     CDS             14750..15852
                     /locus_tag="KJL99_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
BASE COUNT         4040 a         3948 c         4071 g         3793 t
ORIGIN      
        1 tgtttttggg taaatctcca ttcattcaat gaagggaaat tgttatgaaa aaagttctgt
       61 atggcatttt tgccatatct gcgcttgcgg cgacttctgc gtgggctgca cctgtacagg
      121 tgggcgaagc ggcagagtcg gcagcaacgt cggtttcggc ggggagttcc tccgcgacca
      181 gcgtcagcac cgtaagctcg gcggtgggtg tcgcgctcgc ggcaaccggt ggcggtgatg
      241 gttctaatac cgggaccacc acaaccacga ccaccagtac ccagtaataa agtatgtatc
      301 cccaaaataa tttgagtcat tgcatctgtg gcttgaagta tgaagggatt taccataacc
      361 acactccggt gtggttattc tgcccctctg gagaagagtc gtgaagcgac ctgcactcat
      421 tcttatctgc ctgctattac aggcctgttc agccacgact aaagagctgg gcaattcact
      481 gtgggacagt ctgttcggca cgccaggcgt acagctgacg gacgatgata ttcaaaatat
      541 gccctacgcc agccagtaca tgcagcttaa tggcgggccg caattgtttg tggtgctggc
      601 cttcgctgaa gacggacaac aaaaatgggt cactcaggat caagccactc ttgtcacaca
      661 acatggtcgt ctggtgaaga ctttgcttgg cggcgacaac ctgattgaag tgaataacct
      721 tgccgccgac ccgctgatta aacccgcaca aattgttgat ggtgcaacat ggacgcgcac
      781 gatgggctgg accgagtacc agcaggtacg ctacgccacc gcacgctcag tcttcaaatg
      841 ggatggcacc gataccgtca aggtcggcag cgatgaaacc ctggttcgcg tgctggacga
      901 agaagtctcc accgaccagg cgcgctggca taaccgctac tggatcgaca gcgaagggca
      961 aattcgccag tcggaacagt atctcggcgc ggattatttc ccggtgaaaa ccactctcat
     1021 caaggcggca aaacaatgat taaacaaact attgtcgcgt tgcttttgag tgtgggagcg
     1081 tcatcggtct tcgcggcagg aaccgtcaag gtgttcagca atggcagcag tgaggccaaa
     1141 acgctaacgg gcgcagagca tttaatcgat ctggtagggc aacctcgact ggcaaacagc
     1201 tggtggcctg gtgcggtgat tagcgaagag ctggcaacgg cggcagcatt gcgtcagcag
     1261 caggcgttgc tgacacggct ggcagaacag ggcgcagatt ccagcaccga cgatgccgcc
     1321 gccattaacg ccttacgcca gcaaatccag gcattgaaag tgacaggcag gcaaaaaatc
     1381 aatcttgatc ccgatatcgt ccgcgttgcc gaacgcggta acccgccgtt gcagggcaac
     1441 tacacgctgt gggtcggacc accgccgtcc acggtcacgt tgttcgggct tatcagccat
     1501 cctggcaatc agccattcac tccaggtcgc gacgtggcga gctatctctc tgaccagagc
     1561 ctgctcagcg gtgcggatcg cagctacgcg tgggtcgttt acccggacgg acgcacgcaa
     1621 aaagcgccgg tggcttactg gaacaagcgt cacgtagagc cgatgcccgg cagcattatt
     1681 tatgttggcc tcgcagattc cgtctggagt gagacgcctg atgcccttaa cgccgacatt
     1741 cttcagactc tgacgcagcg gataccccaa taatgaaaaa aagacatctg cttagcttac
     1801 tggcgctggg cattagcaca gcttgctacg gcgaaacata tcctgcgccc attggtccgt
     1861 cgcagtcgga tttcggtggc gtaggattat tacaaactcc caccgcgcgt atggcacggg
     1921 aaggggagtt gagtctgaac tatcgcgata acgatcagta ccgttattac tcagcttcag
     1981 tgcaactctt cccgtggctg gaaacaacgc tgcgctacac cgacgtgcgc acccggcagt
     2041 acagcagcgt cgaagcgttc tctggcgatc aaacgtataa agataaagcc ttcgatctca
     2101 aactgcgttt gtgggaagag agttactggc tgccgcaagt ggcggttggt gcgcgggata
     2161 tcggcggtac ggggctgttt gatgcggaat atcttgttgc cagcaaagcc tgggggccgt
     2221 tcgattttac gctcggcctg ggctggggat atctgggcac cagcggtaat gtaaaaaatc
     2281 cgctctgttc agccagtgat aaatattgct atcgcgataa cagctacaaa caggcgggat
     2341 ctatcgacgg tagccagatg ttccacggtc ctgcctcact gtttggcggc gtggaatacc
     2401 agacgccctg gcaaccgctg cgtctgaaac tggagtatga aggtaataat tatcagcagg
     2461 attttgccgg gaagctggag caaaaaagta agtttaacgt cggggcgatt tatcgcgtta
     2521 ccgattgggc cgacgttaac cttagctatg aacgtggcaa cacctttatg tttggcgtca
     2581 cgctgcgcac caactttaac gatctgcgcc cgtcctacat cgataacgcc cgcccgcaat
     2641 atcaaccgca gccgcaggat gccattttgc agcattcggt ggtggcgaat cagttaacgt
     2701 tgctgaaata caacgccgga cttgccgatc cgcagatcca ggcgaaaggc gatacgctgt
     2761 atgttaccgg cgagcaggtg aaatatcgtg attcgcgcga agggatcatc cgtgctaatc
     2821 ggatcgtgat gaacgatctg ccggatggga tcaaaacgat ccgcattacg gaaaatcgcc
     2881 ttaacatgcc gcaagtgacg acggaaaccg atgtcgccag cctgaaaaat catctcgccg
     2941 gagagccgtt gggccacgaa acgacgctgg cgcacaaacg cgtcgagcca gtggttccga
     3001 agtccaccga gcagggctgg tatatcgaca aatcacgctt tgatttccat atcgatccgg
     3061 tgctgaacca gtcggtcggt ggcccggaaa acttttacat gtatcagctg ggcgtgatgg
     3121 gaacggcaga tttgtggctg acggaccatc tactgaccac cggcagcctg tttgcaaatc
     3181 ttgccaacaa ctacgacaag tttaactaca ccaatccacc gcaggactcg cacttaccgc
     3241 gcgttcgtac ccatgtgcgc gagtatgtgc agaacgatgt ctatgtgaat aacctgcaag
     3301 ccaactactt ccagcatctg ggcaacggct tctacggtca ggtctatgga ggttatctcg
     3361 aaaccatgtt tggcggtgcg ggggccgaag tgttgtatcg tccgctggac agtaactggg
     3421 cgtttggtct ggatgccaac tacgttaaac agcgcgactg gcgtagtgca aaagatatga
     3481 tgaaattcac cgactacagc gtgaaaaccg gacatctgac cgcctactgg acgccatctt
     3541 tcgcccagga cgtgttggtg aaagccagcg tcgggcagta tctggcaggg gataaaggcg
     3601 gcacgctgga gatcgctaaa cgctttgaca gcggcgtggt ggtgggtggc tatgccacga
     3661 tcaccaacgt ttcgaaagag gagtacggcg aaggggactt caccaaaggc gtgtatgtct
     3721 cggtaccgtt ggatctcttc tcgtctggcc cgacacgcag ccgtgcggcg attggctgga
     3781 cgccgctgac gcgtgacggt ggtcagcaac ttgggcgtaa gttccagttg tatgacatga
     3841 ccagcgaccg tagcgtaaat ttccgctaag tcatgggaaa ggtgccagtt ttcgcactca
     3901 ctggcaccct cattcttaat gacctgttga gccatcagga acgcagttca cccccggttt
     3961 acgatctggt gtgtaaaggc gaaaacattc tgtcttcctg tccgtcgata tcggttgcag
     4021 gtctggttgc ctgagcgact gggccatcat tgggctggag acattgcttt gcggtaggtc
     4081 ggctttatca gcagtaccga gcggaccagc ataagcagga agaacagaga ctgataacat
     4141 caaagcagca aaataaggct tcatttttac cacctttatc aggttacgtt tcatttgttc
     4201 cagaggaaca ttgtcgattt ttcgcgcatt gctggtggct gggaatcacc tgaatgggtg
     4261 atttttgaat taccggcttt ggtgcggttt gtcttgccgg atgcgccgcc aggcgcggct
     4321 tatccggcct acgggtaggt atatccggct ttggtggagg cgcgctccaa atccaggttg
     4381 aacaaaacat acacaaaaaa tatagatctc cgtcacattt ttgcgttata caggaagctc
     4441 gccactgtga aggaggtact gctatgacgt cactctctcg tccgcgcgtg gagtttatct
     4501 ccaccatttt gcagaccgta ctcaatcttg gcctgctgtg cctcggcctg attttggttg
     4561 tcttcctcgg caaagaaacg gtgcatctgg ctgatgtgct gttcgcgcca gaacaaacca
     4621 gcaaatatga gctggtagaa ggactggtgg tttactttct ctatttcgaa tttatcgcgc
     4681 tgattgtgaa gtactttcag tccggttttc acttcccgct acgttacttt gtctatattg
     4741 ggatcaccgc cattgtgcgc ttgatcatcg tcgatcataa atcgccactg gatgtgttga
     4801 tctactcggc tgcgatcctg ttactggtga tcaccctgtg gctgtgtaat tcgaaacggc
     4861 tgaagcggga gtaaaaagac agcacgccga aatggcgcgg cgtgctggac aggaagatta
     4921 cagcgtagca gtttgttgtg ttttcttcgt ttccggttcc cagagcgctt ccagctcctc
     4981 aagggtttta cctttggttt ccgggacaaa tttccacata aacagtgctg ccagaacgcc
     5041 catacaaccg taaatccagt aggagaaacc gttgtggaaa tgggccacca gccaggagtt
     5101 tttgtccatc atcgggaagg tccaggagac gaagtagttc gccagccact gggccgccac
     5161 cgcgattgcc agcgctttac cacgaatagc attcgggaag atttccgaca gcagtaccca
     5221 gcataccgga ccccaagaca tggcaaaggc ggcaacatag aacagcatcg acagtagcgc
     5281 cacaataccc gatgcctgag tgtaaaacgc ggtaccgagg ctaaacatac cgattgccat
     5341 tccgagtgcg ccgataattt gcagtggctt acgaccaaat ttatccacag tcataattgc
     5401 cagtacggta aaggtgaggt tgataactcc gacaataatg gtctgcaaca gcgcgatatc
     5461 cgtgctggcc cccagcgttt tgaacacttc cggcgcgtag tacagcacca cattgatgcc
     5521 gacaaattgc tggaagatgg agagcataac gccgattaca atcacgccca cgccaaacat
     5581 cagcagacga ccaccggttt tgcggccatg atccagggag tgtttaattt cctgtactgc
     5641 ctgagttgca agcgtgttgc ccataatttt gcgcaggata ctttccgtct gttcttgctt
     5701 gccgcgtgac atcagccagc gaggactttc tggcacggta tacagcagca ttaagaacag
     5761 cagtgcaggg atacattccg aggcaaacat ataacgcctg ccgtcagtat tcagccagct
     5821 ggcatcaccg gaacgggcaa taaaatagtt tacgcagtaa actaaaagtt gcccgaaaat
     5881 aatcgcaaac tggttaaatg aaaccagttt tccgcgaata tgagctggag ccagttccgc
     5941 aatatacatt ggtgaaagca ttgaggctaa accaacgcca ataccgccaa taatacgata
     6001 aataacaaat tccgggacat aacctgccag ataaataggt actgtgttgt ccgggtttat
     6061 agaggtaaaa ccaagttctg gccaggcaga acctacacca gaaataaaaa acaggacagc
     6121 agaaatctta agtgaatcac gacggccgaa gcggttactg caataaccac cgagggcacc
     6181 gccgatgatg caaccaatca gagcgctggc tacgcaaaat cctaacaggg agttggcagc
     6241 ggattcactt aagttttgtg gagcaacaaa gacggtattg agtgactcaa cagtaccgga
     6301 aataacggcg gtatcgtagc caaataataa accacctaat gtagcgacta aggtaatcga
     6361 aaatatataa ctggaattat actgggtatt cattcagacc tgccttagac cattctgatc
     6421 ttttatcagt gcattgatgt tggaattgtc aggcagttaa ggcaattatc attttttaca
     6481 atgcgtttac gtaatgtctt ttctgtgatc ttaattgtga taattatcaa aaattgatga
     6541 aacaatacta ttgccgtgac tcagagcacg aaagagaatt atcgtaagtg ggaaaacaaa
     6601 taacgtaaaa ataataagtt ctatgatggg aatattaacc ggcgaacgat tcagattgca
     6661 gacgaaagaa aaaaaggcgc tccgtggagc gccgaataat agtcacaagt tgggataacg
     6721 taagttgagg gtgcagcggc ataacattgg cagaacaaca tctttaacct ttcacaccac
     6781 ctgccgtcag gccgttgacc agccagcgtt gagccagcaa gaagacgatg gtgatcggta
     6841 atgcagacat cacggcagcg gcggcaaagt caccccacag gtagttttgc gggttgaggt
     6901 attgctgcat ccccacggcc agggtgtagc tgtttacgtc acgcagtaac agcgacgcga
     6961 ccggaacttc agtaatggca gcgataaacg acaggatgaa taccaccgcc agaatcggta
     7021 ctgacagcgg caacaggaca aggcggaagg cctgccacgg tgtcgcacca tccagcgcag
     7081 ccgcttcttc cagcgaactg tcgatggttt cgaaatagcc tttgatggtc cagacatgca
     7141 gcgcaatccc acccagatac gcgaaaatta cgccgccgtg agtattcagg ccaatgaatg
     7201 gaatgtactc acccagacga tcaaacaacg catacaacgc gaccagtgaa agtactgccg
     7261 ggaacatctg gaaaatcagc attcctttca gcagcgtcgc tttgcctgga aagcgcatac
     7321 gggcgaaagc gtaggcgcag gtggtggaga gcgccacaat gccaatcgcg gaaatcccag
     7381 cgacctttac cgagttccac agccacagca gtaccgggaa tggcggtggc gtaatgcgac
     7441 catcagcctg ttcaacgcta aaacctaacg ccagtttcca gtgatcccag gagatttgct
     7501 ccgggatcag gctaccggtc gcgaagtttc cctgacgcag cgagatagcg acgaccatca
     7561 gcagcgggaa cataatcgcc gcgataaaaa gtagcagtag caggtgagta ataaacaaac
     7621 gtgctttttg cgatttcggt tggaccattg ccatttttgt tatctccctt aatcaaactt
     7681 cattcgcgtg gctttcaggt tcactatcgc cagcgcaccc accagcagga agatcagcgt
     7741 ggcaattgct gccgccagac cgaagtcctg acccccgccg ccttcaaaag cgatacggta
     7801 ggtgtagtta acaagcaggt cggtataacc ggctggcgtg gtcgtgccaa gacgatccgg
     7861 gccgccgttg gttaacagct gaatcagcac gaagttgtta aagttaaagg cgaagctggc
     7921 gatcatcagc ggcgtcagcg gtttaatcag cagcggcagc gtaatcttaa agaagttctg
     7981 gaacggacct gcgccatcca ttgctgaggc ttcatacaaa tcgtccggaa tcgctttcag
     8041 caagcccatg cagaggatca tcatgtacgg ataacccagc caggtattga cgataatcag
     8101 catcgtgcga gcggtggtcg gatcgctgaa ccaggcgggc ttcacgccaa acagcgcgct
     8161 caacatcatg ttgatttcac cgaagctctg gttaaacaat cctttgaaaa tcaagattga
     8221 aatgaacgat ggcaccgcgt agggcagaat cagcaggacg cgatagaccg ctttgccgcg
     8281 caacgcttcc cactgcacca gacatgccag aaccatgccg accgccaccg ttaaaaagac
     8341 agtgatcagc gagaacacca cggtccagac gaaaatggcg aggaacggtt tctgaatgcc
     8401 ttcgtcggta aagacgcggg taaagttttt ccagccggtg gtcacggtgt aaccggggct
     8461 tagcttttca tccccccagt tgccgtcggc ggtaatggac tggtaaaagc caatttggtt
     8521 attcggacga tatttcacgc cactctgatt attcgtcaac gtgccgtcac cgtcgagtgt
     8581 gtagagcggc tgcgtgccag aaaactggcg cagggagctc atcatcactt tgttgccatc
     8641 cggcagaatg gcggtaatgt cactcagcgc ctgacgattc tgggtaatca cgcgcagatt
     8701 cgcgcgttcg ccttcgggct gggcggtcgt ttctttcagt tgcagttttt gctcgccgcc
     8761 aaatttaaac gcgtcggaga ggtaattttt gccggtttcg ccgtcgctga gcgccagttg
     8821 ccactcatcg cccgccgggt aaagaccaaa gttataggtt ttgcctgctt gccaggagcg
     8881 atctaacaac acttcctgcg cacgttcaaa agtcagctgg ttagtgctgc tgtagttggt
     8941 gaaggcaatg gcgatggtgc agaccagagg gaagaggacg aataatccca ttccagccat
     9001 tcccgggtaa acatagcgcc aggcgtaggc tttacgattg gcgaaaatat acagccccgc
     9061 tgaactcaat atcagcgtgg taatggcgaa caggtattcc ccttgtgcgt acattaaaac
     9121 aacaaggtaa cccaccagca ggccgagcag acctagcact gaccatttca gcgcgtcgct
     9181 ttgccaccaa tgtttctttt taatgacatc catggggttc ttcctcattc caggacggat
     9241 aaggctttca cgccttatcc gacaacaact gcctgatgcg acactgatgc gtcttatcag
     9301 gcctacatgc gtttcagttt tgtaggccgg acaaggcgtt cacgccgcat ccggcatttc
     9361 acagcattac ttggtgatac gagtctgcgc gtctttcagg gcttcatcga cagtctgacg
     9421 accgctggcg gcgttgatca ccgcagtacg tacggcatac cagaaagcgg acatctgcgg
     9481 gatgttcggc atgatttcac ctttctgggc gttttccatg gtggcggcaa tacgtggatc
     9541 tttcgccaac tcttcctcgt aagacttcag cgctacggca cccagcggtt tgtctttatt
     9601 aaccgcttcc agaccttcat cagtcagcag atagttttcg aggaactctt tcgccagctc
     9661 tttgttcgga ctggcggcgt taataccggt gctcagcacg ccaacgaacg gtttagatgg
     9721 ttgacccttg aaggtcggca gtaccgttac accataattc actttgctgg tgtcgatgtt
     9781 ggaccatgcc cacgggccgt tgatggtcat cgctgtttcg cctttattaa aggcagcttc
     9841 tgcgatggag taatcggtgt ctgcattcat gtgtttgttt ttaatcaggt caaccaggaa
     9901 ggtcagaccc gctttcgcgc cagcgttatc cacgcccacg tctttaatgt cgtacttgcc
     9961 gttttcatac ttgaacgcat aacccccgtc agcagcaatc agcggccagg tgaagtacgg
    10021 ttcttgcagg ttgaacatca gcgcgctctt acctttcgct ttcagttctt tatccagcgc
    10081 cgggatctct tcccaggttt ttggcgggtt cggcagcagg tctttgttat aaatcagcga
    10141 taacgcttca acagcgattg ggtaagcaat cagcttgccg ttgtaacgta cggcatccca
    10201 tgtaaacgga tacagcttgt cctggaacgc tttgtccggg gtgatttcag ccaacaggcc
    10261 agattgagcg tagccaccaa agcggtcgtg cgcccagaag ataatgtcag ggccatcgcc
    10321 agttgccgca acctgaggga atttctcttc cagtttatcc ggatgctcaa cggtgacttt
    10381 aattccggta tctttctcga atttcttacc gacttcagcg agaccgttat agcctttatc
    10441 gccgttaatc cagattacca gtttaccttc ttcgattttg gcgagagccg aggcggaaaa
    10501 catcatcgtc gttaatgcgg ataatgcgag gatgcgtgca cctgttttta ttttcataat
    10561 ctatggtcct tgttggtgaa gtgctcgtga aaacacctaa acggactcta gtttctttat
    10621 acggcaacct ctttccatcc tccttgcccc tacgccccac cgttgctttg tgtgatctct
    10681 gttacagaat tggcggtaat gtggggatgc gcacataaaa tcgccacgat ttttgcaagc
    10741 aacatcacga aattccttac atgacctcgg tttagttcac agaagccgcg ttctcatcct
    10801 cccgcctcct cccccataaa aaagccaggg ggtggaggat ttaagccatc tcctgatgac
    10861 gcatagtcag cccatcatga atgttgctgt cgatgacagg ttgttacaaa gggagaaggg
    10921 catggcgagc gtacagctgc aaaatgtaac gaaagcctgg ggcgaggtcg tggtatcgaa
    10981 agatatcaat ctcgatatcc atgaaggtga attcgtggtg tttgtcggac cgtctggctg
    11041 cggtaaatcg actttactgc gcatgattgc cgggcttgag acgatcacca gcggcgacct
    11101 gttcatcggt gagaaacgga tgaatgacac tccgccagca gaacgcggcg ttggtatggt
    11161 gtttcagtct tacgcgctct atccccacct gtcagtagca gaaaacatgt catttggcct
    11221 gaaactggct ggcgcaaaaa aagaggtgat taaccaacgc gtcaaccagg tggcggaagt
    11281 gctgcaactg gcgcatttgc tggatcgcaa accgaaagcg ctctccggtg gtcagcgtca
    11341 gcgcgtggcg attggccgta cgctggtggc cgagccaagc gtatttttgc tcgatgaacc
    11401 gctctccaac ctcgatgctg cactgcgtgt gcaaatgcgt atcgaaatct cccgtctgca
    11461 taaacgcctg ggccgcacaa tgatttacgt cacccacgat caggtcgaag cgatgacgct
    11521 ggccgacaaa atcgtggtgc tggacgccgg tcgcgtggca caggttggga aaccgctgga
    11581 actgtaccac tatccggcag accgttttgt cgccggattt atcggttcgc caaagatgaa
    11641 cttcctgccg gtaaaagtga ccgccaccgc aatcgatcaa gtgcaggtgg agctgccgat
    11701 gccaaatcgt caacaagtct ggctgcctgt tgaaagccgt gatgtccagg ttggagccaa
    11761 tatgtcgctg ggtattcgcc cggaacatct actgccgagt gatatcgctg acgtcatcct
    11821 tgagggtgaa gttcaggtcg tcgagcaact cggcaacgaa acccaaatct atatccagat
    11881 cccttccatt cgtcaaaacc tggtgtaccg ccagaacgac gtggtgttgg tagaagaagg
    11941 tgccacattc gctatcggcc tgccgccaga gcgttgccat ctgttccgtg aggatggcac
    12001 tgcatgtcgt cgactgcata aggagccggg cgtttaagca ccccacaaaa cacacaaagc
    12061 ctgtcacagg tgatgtgaaa aaagaaaagc aatgactcag gagatagaat gatgattact
    12121 ctgcgcaaac ttcctctggc ggttgccgtc gcagcgggcg taatgtctgc tcaggcaatg
    12181 gctgttgatt tccacggcta tgcacgttcc ggtattggct ggacaggtag cggcggtgaa
    12241 caacagtgtt tccagactac cggtgctcaa agtaaatacc gtcttggcaa cgaatgtgaa
    12301 acttatgctg aattaaaatt gggtcaggaa gtgtggaaag agggcgataa gagcttctat
    12361 ttcgacacta acgtggccta ttccgtcgcg caacagaatg actgggaagc taccgatccg
    12421 gccttccgtg aagcaaacgt gcagggaaaa aacctgatcg aatggctgcc aggttccacc
    12481 atctgggcag gtaagcgctt ctaccaacgt catgacgttc atatgatcga cttctactac
    12541 tgggatattt ctggtcctgg tgccggtctg gaaaacatcg atgttggctt cggtaaactc
    12601 tctctggcag caacccgctc ctctgaagct ggtggttctt cctcttttgc cagcaacaat
    12661 atttatgact ataccaacga aaccgcgaac gacgttttcg atgtgcgttt agcgcagatg
    12721 gaaatcaacc cgggcggcac attagaactg ggtgtcgact acggtcgtgc caacctgcgt
    12781 gataactatc gtctggttga tggcgcatcg aaagacggct ggttattcac tgctgaacat
    12841 actcagagtg tcctgaaggg ctttaacaag tttgttgttc agtacgctac tgactcgatg
    12901 acctcgcagg gtaaaggtct gtcgcagggt tctggcgtcg cgtttgataa cgaaaaattt
    12961 gcctacaata tcaacaacaa cggtcacatg ctgcgtatcc tcgaccacgg tgcgatctcc
    13021 atgggcgaca actgggacat gatgtacgtg ggtatgtacc aggatatcaa ctgggataac
    13081 gacaacggca ccaagtggtg gaccgttggt attcgcccga tgtacaagtg gacgccaatc
    13141 atgagcaccg tgatggaaat cggctacgac aacgtcgaat cccagcgcac cggcgacaag
    13201 aacaatcagt acaaaattac cctcgcacaa caatggcagg ctggcgacag catctggtca
    13261 cgcccggcta ttcgtgtctt cgcaacctac gccaagtggg atgagaaatg gggttacgac
    13321 tacaacggcg atagcaaggt taacccgaac tacggcaaag ccgttcctgc tgatttcaac
    13381 ggcggcagct tcggtcgtgg cgacagcgac gagtggacct tcggtgccca gatggaaatc
    13441 tggtggtaat agcaaaacct gggccggata aggcgtttac gccgcatccg gcataaaaac
    13501 aggttgtcat tatctgaaag gggcgaaagc ccctctgatt atcgggttta gcgcgctatt
    13561 gcctggctac cgctgagctc cagattttga ggtgaaaaca atgaaaatga ataaaagtct
    13621 catcgccctc tgtttatcag cagggttact ggcaagcgcg cctggaatta gccttgccga
    13681 tgttaactac gtaccgcaaa acaccagcga cgcgccagcc attccatctg ccgcgctgca
    13741 acaactcacc tggacaccgg tcgatcaatc taaaacccag accacccaac tggcgaccgg
    13801 cggccaacaa ctgaacgttc ccggcatcag tggtccggtt gctgcgtaca gcgtcccggc
    13861 aaacattggc gaactgaccc tgacgctgac cagcgaagtg aacaaacaaa ccagcgtttt
    13921 tgcgccgaac gtgctgattc ttgatcagaa catgacccca tcagccttct tccccagcag
    13981 ttatttcacc taccaggaac taggcgtgat gagtgcagat cggctggaag gcgttatgcg
    14041 cctgacaccg gcgttggggc agcaaaaact ttatgttctg gtctttacca cggaaaaaga
    14101 tctccagcag acgacccaac tgctcgaccc ggctaaagcc tatgccaaag gcgtcggtaa
    14161 ctcgatcccg gatattcccg atccggttgc tcgtcatacc agcgatggct tactgaaact
    14221 gaaagtcaaa acgaactcca gctccagcgt gttggtagga ccgctatttg gttcttccgc
    14281 tccagctccg gttacggtag gcaacacggc ggcaccagct gtggctgcac ctgctccggt
    14341 gccggtgaag aaaagcgagc cgatgctcaa cgacacggaa agttatttta ataccgcgat
    14401 caaaaacgct gtcgcgaaag gtgatgttga taaggcgtta aaactgcttg atgaagctga
    14461 acgcctggga tcgacatctg cccgttccac ctttatcagc agtgtaaaag gcaaggggta
    14521 attacgcccc acagtgctga ttttgcaaca actggtgcgt ctcctggcgc accttttttt
    14581 atgcttcctt cctgggatat gagcgatttt ttatagtact tcacttcttc ttcactaaga
    14641 atgtccatta tttcaatgca ttatcagaga ttcttttcct ttcgccggta gtgtctggac
    14701 gctcaggcta cttttccagg ttattttatt tctgtattac agaggtttta tgataggtca
    14761 tatcacaaat tctggcggca ataactcttt gatgaaacat gatgtggtgc aaggaaataa
    14821 tatagtagat cttgatttac tacgtaattt caatggggtg acaggtttaa acagagatta
    14881 ctttatttat atcagtgata tatttttaaa tataaaacaa cggaacgaaa aaaatcatgc
    14941 aataaatatg ttacgtgaag tctcaattag taatgatact ataagtgtaa aattctacag
    15001 aaatgaaaaa atagaatgcg cttgtaattt tctgatggat aaagatgcgc aggggtatat
    15061 cgacctgtct gattttgatt taacaaattg tcattttaaa ggtgacgtta tttctaaggt
    15121 gtctttttta tcatcaaatc tacaacatgt aactttcgaa tgtaaagaaa ttgagaattg
    15181 caattttact aaggcaacag ttgataatgt catatttaaa tgtcgacgtt tacacaatgt
    15241 gatttttctc aaaacgagtg gcgaatgtgt tgattttagc caaaatattc ttgatacagt
    15301 tgacttctcg cagagtcaac ttggtcatag taattttcgc gaatgtcaga ttagaaattc
    15361 aaacttcgat aattgttatc tttacgcttc gcacttcacc agagcagagt ttctgtctgc
    15421 caaagaaata tcatttatta aatcgaattt gacagctgtt atgtttgatc atgtgcgaat
    15481 gtcgacaggg aatttttaag attgcattac agaacaattg gaattaacta ttgattattc
    15541 agatatattt gggaatgaag atctcgatgg ttatatcaat aacattataa aaatgattga
    15601 tacattgcca gataatgcaa tgatattgaa atccgttctg gcagtaaaac tggtgatgca
    15661 attaaaaatt ctcaatattg ttaataaaaa ctttattgag aatatgaaga aaacatttag
    15721 ccattgtcct tatataaaag atccaattat acgtagttat atacattctg gggaagatga
    15781 caagttcgat aattttatgc gtcaacatcg attcagtaag gtgaatttcg atacccaaca
    15841 gatgatccat tt
//
LOCUS       DAEBXN010000094        15412 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.094, whole genome shotgun sequence.
ACCESSION   DAEBXN010000094 DAEBXN010000000
VERSION     DAEBXN010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15412
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.094"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(<1..1177)
                     /locus_tag="KJL99_003105"
     CDS             complement(<1..1177)
                     /locus_tag="KJL99_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HBD4437744.1"
                     /translation="MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRF
                     GGKEKMLALGKYPEVSLADARARRDEARKLLANGVDPSENKKAVKVEQEQEAITFEVV
                     ARDWHASNQKWSASHSARVLKSLEDNLFTAIGKRNIAELKTRDLLVPIKAVESSGRLE
                     VAARLQQRTTAIMRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLHRID
                     HYSGRPLTRLAVELTLLVFIRSSELRFARWSEIDFETAMWTIPGEREQLEGVKHSQRG
                     SKMRTPHLVPLSRQALSILEKIKSMSGNRELIFVGDHDPRKPMSENTVNKALRVMGYD
                     TKVEVCGHGFRTMACSSLIESGLWSRDAVERQMSHQERSSVRAAYIHKAEHLGERRLM
                     "
     gene            complement(1376..1460)
                     /locus_tag="KJL99_003106"
     tRNA            complement(1376..1460)
                     /locus_tag="KJL99_003106"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1424..1426),aa:Leu,seq:caa)
     gene            1656..2675
                     /gene="ahr"
                     /locus_tag="KJL99_003107"
     CDS             1656..2675
                     /gene="ahr"
                     /locus_tag="KJL99_003107"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HBD4437745.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2804..4306
                     /locus_tag="KJL99_003108"
     CDS             2804..4306
                     /locus_tag="KJL99_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HBD4437746.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHTVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVFGQLGNRVQHALRAFTPKDQKAV
                     KTAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(4425..5507)
                     /gene="lptG"
                     /locus_tag="KJL99_003109"
     CDS             complement(4425..5507)
                     /gene="lptG"
                     /locus_tag="KJL99_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HBD4437747.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEVLGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTDLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5507..6607)
                     /gene="lptF"
                     /locus_tag="KJL99_003110"
     CDS             complement(5507..6607)
                     /gene="lptF"
                     /locus_tag="KJL99_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HBD4437748.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            6874..8385
                     /gene="pepA"
                     /locus_tag="KJL99_003111"
     CDS             6874..8385
                     /gene="pepA"
                     /locus_tag="KJL99_003111"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HBD4437749.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGQAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMTNHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8519..8962
                     /gene="holC"
                     /locus_tag="KJL99_003112"
     CDS             8519..8962
                     /gene="holC"
                     /locus_tag="KJL99_003112"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HBD4437750.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGSAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            8962..11817
                     /gene="valS"
                     /locus_tag="KJL99_003113"
     CDS             8962..11817
                     /gene="valS"
                     /locus_tag="KJL99_003113"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HBD4437751.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(11871..13067)
                     /locus_tag="KJL99_003114"
     CDS             complement(11871..13067)
                     /locus_tag="KJL99_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HBD4437752.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTAYCIKYAILAFLALLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGVM
                     PSLPFL"
     gene            13260..13763
                     /locus_tag="KJL99_003115"
     CDS             13260..13763
                     /locus_tag="KJL99_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBD4437753.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIASVIRQVYAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYNGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(13809..14225)
                     /gene="rraB"
                     /locus_tag="KJL99_003116"
     CDS             complement(13809..14225)
                     /gene="rraB"
                     /locus_tag="KJL99_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HBD4437754.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            14387..15400
                     /gene="argF"
                     /locus_tag="KJL99_003117"
     CDS             14387..15400
                     /gene="argF"
                     /locus_tag="KJL99_003117"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HBD4437755.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
BASE COUNT         3978 a         3933 c         3969 g         3532 t
ORIGIN      
        1 acatcaacct acgctcccca agatgttccg ccttatggat gtaagctgct cgcacagagc
       61 tacgctcctg gtgactcatc tgccgctcta ccgcatccct cgaccataat cccgactcaa
      121 tcaacgaact acaagccatc gtcctgaagc catgcccaca aacctcgacc ttcgtgtcat
      181 accccattac gcgcaaagcc ttgttcaccg tattctcact catcggctta cgtggatcgt
      241 gatcgcccac aaaaatcagc tctcgatttc cgctcatgct tttgatcttt tccaaaatac
      301 ttagggcctg ccgcgacaaa ggtacaaggt gtggtgtccg catcttcgag ccacgctgag
      361 aatgcttgac tccttccagt tgctcacgct cacctgggat tgtccacata gccgtttcaa
      421 aatctatttc tgaccagcgg gcaaaacgca gctcgctcga acggatgaaa accaataaag
      481 tgagttcaac cgccagtcga gttaacggtc tgccagaata gtgatctatg cgatgaagta
      541 attcaggtat acggttcagt tcaagagctg cacggtgctg tcttttcgcc gtagcaaccg
      601 cacctgcaat ctcttgcgcg gggttgtagt cgattaagcc gctctgaacg gcaaagcgca
      661 taatcgcggt agtacgctgc tgtaaacggg cggcaacttc gagccgcccg gatgactcga
      721 ctgctttaat gggtacaaga agatcccgcg tcttcagttc cgcaatgttc cgcttaccga
      781 tagcagtaaa gagattatcc tccagacttt tcaaaacacg agcgctatgc gatgccgacc
      841 acttctgatt gctggcgtgc cagtctctgg cgactacttc aaacgttatc gcctcttgtt
      901 cctgttctac cttaacagct ttcttgtttt cactgggatc gacaccatta gctaaaagct
      961 tacgagcctc atcccggcgt gccctggcat ccgccaacga cacttcgggg tatttcccca
     1021 atgccagcat cttctctttg ccgccaaagc gataacgtag ccgccagtat ttggagccgt
     1081 tagggtgaac cagcaaaacc atgccttcac cgtcggtcag cttataggct tttgcttcag
     1141 gcttagccga acgaaccttc acatcactca gagccatgat gagtatcctt tcaagggttc
     1201 tgtgtgggta caaacattat cgaaccggga aatacccgca gttgtacccg catcagtaag
     1261 ttgatgtaga ttgaatcagg ttgacttagg ttgagtgaaa aagcgaggaa agccttgcgg
     1321 atactggatt ttaggcacaa aaaaagacgt ccgttgacgt ctattgatgt tccgatggtg
     1381 ccgaaggccg gactcgaacc ggcacgtatt tctacggttg attttgaatc aactgcgtct
     1441 accgatttcg ccacttcggc actgaagagg tatgcggaaa acgttgtgga ttatacctgt
     1501 cgcacgccac catgcaagcg ccagcttgca cccacctcgc taagtgctga aattttcagc
     1561 attacactca gcgatatgta acctttgtca cactccaggc accccgccct gccatgctct
     1621 acacttccca aacaacacca gagaaggccc caaaaatgtc gatgataaaa agctatgccg
     1681 caaaagaagc gggcggcgaa ctggaagttt atgagtacga tcccggtgag ctgaagccac
     1741 aagatgttga agtgcaggtg gattactgcg ggatttgtca ttccgatctg tcgatgatcg
     1801 ataacgaatg gggattttca caatatccgc tggttgccgg gcatgaggtg attgggcgcg
     1861 tagtggcact cgggagcgcc gcgcaggata aaggtttgca ggtcggtcag cgtgtcggga
     1921 ttggctggac ggcgcgtagc tgtggtcact gcgacgcctg tattagcggt aatcagatca
     1981 actgcgagca aggtgcggtg ccgacaatta tgaatcgcgg tggctttgcc gagaagttgc
     2041 gtgcggactg gcaatgggtg attccactgc cagaaaatat tgatatcgag tccgccgggc
     2101 cgctgttgtg cggcggtatc acggtcttta aaccactgtt gatgcaccat atcactgcta
     2161 ccagccgcgt tggggtaatt ggtattggcg ggctggggca tatcgctata aaacttctgc
     2221 acgcaatggg atgcgaggtg acggccttta gttctaatcc ggcgaaagag caggaagtac
     2281 tggcgatggg tgccgataaa gtggtgaata gccgcgatcc gcaggcactg aaagccctgt
     2341 cggggcagtt tgatctcatt atcaatactg tgaacgtcag cctcgactgg cagccttatt
     2401 ttgaggcgct gacctatggc ggtaatttcc atacggtcgg tgcggttctc acgccgctgt
     2461 ctgttccggc ctttacgtta attgcgggcg accgcagcat ctctggttct gctaccggca
     2521 cgccttatga gctgcgaaag ctgatgcgct ttgccgcccg cagcaaggtt gcgccgacaa
     2581 ccgaactgtt cccgatgtcg aaaattaacg acgccattca gcatgtacgc gatggtaaag
     2641 cgcgctaccg agtagtcctg aaagccgact tctgatccct ttgccgggtg ctgtcgcccg
     2701 gcacttaaat ctctttggaa ggcgtctcgc aatctgctga atttagcggt gaagagtgca
     2761 cagccgttgc ctatactcca ggcaggaaac tggaggaaat ctcatgagcg aacccctgtt
     2821 aattgcccgc acgccggaca cagaactgtt tttactgccg ggaatggcta accgtcacgg
     2881 gctgattact ggcgcaacgg ggacgggtaa aaccgttacg ctgcaaaaac tggcagagtc
     2941 attgtcggaa atcggcgtgc cggtgtttat ggctgatgtg aaaggcgatc tgaccggtat
     3001 cgcgcaggca ggaacggcgt cggaaaaact gctcacaagg cttaaaaata tcggcgtcaa
     3061 tgactggcaa ccgcatgcca atccggtggt ggtgtgggat atctttggcg agaaaggcca
     3121 cacggtgcgg gcgacggttt cggatctggg gccgctgttg ctggcgcgac tgttgaatct
     3181 caacgatgtg caatctggcg tgctgaatat tatcttccgc attgcggacg atcagggatt
     3241 gttgctgctc gactttaaag atttgcgggc gattacccag tacatcggcg ataacgccaa
     3301 atccttccag aatcagtacg gaaatatcag tagcgcatcg gttggtgcca tccagcgcgg
     3361 attactgtcg ctggaacagc aaggcgcagc acacttcttt ggtgagccga tgctggatat
     3421 caaagactgg atgcgcaccg atgccaacgg taaaggcgtt atcaatatcc tcagcgccga
     3481 gaaactttat cagatgccga aactgtacgc cgccagcctg ctgtggatgc tttcagagtt
     3541 gtatgaacaa ttgccggaag cgggcgatct ggagaaacca aaactggtgt ttttcttcga
     3601 cgaggcacat ctgctgttta acgatgcacc gcaggtactg ctggataaga ttgagcaggt
     3661 gataaggctt attcgctcaa aaggcgtggg cgtctggttc gtttcgcaaa acccgtctga
     3721 tattccggat aatgtgttcg gccagctcgg taatcgtgtt cagcacgctt tgcgggcttt
     3781 tacgccaaaa gatcagaaag cagtgaagac agcggcgcaa accatgcggg ccaatccggc
     3841 atttgatacc gaaaaggcaa tccaggaact ggggaccggc gaggcgttaa tctcgtttct
     3901 cgatgcaaaa ggaagtcctt ctgtggtgga acgggcgatg gtgatcgcac cttgttcgcg
     3961 aatggggccg gtgacggaag atgagcgtaa tggcctgatt aatcactccc cggtgtatgg
     4021 caaatatgaa gatgaggtgg accgcgagtc cgcctatgag atgctgcaaa aaggctttca
     4081 ggccagtacc gagcagcaaa ataatccccc cgtgaaaggg aaagaggtgg cggtggatga
     4141 cggtattctt ggtggattga aggatatttt gtttggcact accggaccac gcggcgggaa
     4201 gaaagatggt gtggtgcaaa caatggcgaa aagcgccgct cgccaggtga cgaatcagat
     4261 tgtacgcggg atgttgggga gtttgctggg ggggagaaaa agataagtca gacgtgatct
     4321 gtgcccctca ccctaaccct ctccccaaag gggcgagggg accgttcgaa ctcgtatttg
     4381 actttgtcaa caataataaa gggagctttc gctcccttgt tagattacga ttttctcatt
     4441 aacagccaca ggctgattaa gaagaagctg gcacttggca acagtgcgcc gatgatcggc
     4501 gggatgccgt aaaccaacgt cagcgggccg aagatctggt ccagtacgta gaagacaaaa
     4561 ccgaagctga tacctgtgac cacacgcacg cccatcggta cgctacgcag tgggccaaag
     4621 ataaacgaca gcgccatcag catcatcacc gccacggata gcggctggaa gattttgctc
     4681 cacatgttga gctgataacg tccggcatcc tgaccgctcg acttcagata cttcacgtag
     4741 ttgtgcaaac cactgatgga gagtgcatcc gggtccagcg ccaccacgcc cagtttgtct
     4801 ggcgtgaggt cggttttcca ggtgccgctc accgtctgcg aaccggtaat ctgtttcgga
     4861 ttggtcagat cagattcatc aacctgcgac agacgccaga ctttatgttc cgggtcaaac
     4921 ttcgcggtag cggcataacg tacggattgc agacgacgat tctcgttaaa ggcataaata
     4981 ctgatgccac ctaacacctc gtcaccttta acccgctcaa tgtagacgaa gttgttgcca
     5041 tctttcgccc ataaaccttg ctgggtagag agcaacgagc cgccgtacat cgcctgcgca
     5101 cggtagttac gcgccatctg ttcgccctgc ggcgcgaccc attcgccaat cgccatcgtc
     5161 agcaagacca gtggaatggc ggttttcatc accgacagcg ctacctgcat acgggtaaaa
     5221 ccagaagcct gcatcaccac cagttcgctg cgctgcgcca gcatcccaag accaagcaac
     5281 gccccaagaa gagccgccat cgggaagaag atctgcacat ctttcggcac gctcagcaag
     5341 gtatacattc ctgcgcctaa cgcgtcgtaa ctcccctgcc cggctttttt cagctgatcg
     5401 acaaatttga taatgcccga cagcgacacc agcatgaaca gcgtcatcat gatggtggtg
     5461 aaaatagttt taccgatata gcggtcaagt acgccaaaag gttgcatcac accgctcctt
     5521 tacgcgaaaa actggcgcgc aggcggcgga ccggcacggt gtcccaaagg ttgagaacaa
     5581 tcgccaaagc cagataaatc aggttaacgg tccacatcca cagtgtcggg tccagcttac
     5641 ctttaccgcc gttcgatttc agggaggtct ggatcaggaa gaaaagtaga tacagcagca
     5701 tggctggcag catcgacagt acgcgtccct gacgcgggtt aaccacgctc aacggtacaa
     5761 ccataagtgc catcataaac acggtgaata ccaacgtgat acgccagttc agttctgcgc
     5821 gagcacgatc ggtgtcagtg ttccacaatg tgcgcatgtc catctggtcg gtatcgttcg
     5881 ggtcgagcgc caccgcctgg tgaccaatga tcgcctgata atcctggaaa tccgtaatgc
     5941 ggaaatcacg taacagtgca gtgccttcga agcgcgttcc ctggttgaga gtgacgacct
     6001 gggagccgtc gcgcagctgg gttaaatgtc cggaatcggc caccaccaca gaaggacgtg
     6061 cattaccttt tggtcgaatt tgtgcgagga acacatcttt gaaatcgctg ccgtcaacgc
     6121 tttcgatgaa cagcaccgag ctgccattag tcgcttgctg gaattgccct tgcgccagcg
     6181 ccgccatgcc agggttcgct ttcgcttctg ctaacacttc atcctgatga cgcgatgacc
     6241 acggtcccgc ccacatcacg ttaaccgccg cgacgattgc cgtgaatacc gcaaggatca
     6301 ttgccgcttt caccagaacc gctttgctca agccgcaggc atgcattacc gtaatttcac
     6361 tttcggtata cagtttgccc agcgtcatca gcagcccgag gaacaggctt aatggcagga
     6421 taagctgcgc catttccggc acgcccaacc cgagaaggga gagcaccaga ttcgccggaa
     6481 tatcgccgtc aaccgctgcg ccgaggatcc tcactaactt ttgacagaag aagatcaaaa
     6541 gcaagatgaa gagtatcgcc agctggcttt tgagcgtctc ccgcaccaga tatcttatga
     6601 ttatcacttt aaatacgccc gtaaaaactc gtcttttgca ggattttagc ttgtttcatg
     6661 gcttaaacgt catttattct cttgagtcgt cgaaatcgtc gctaagataa ttatactcaa
     6721 cggattcacc tctcagattt tgttctgacg tgccaatgcc gtaataacgt taagattaac
     6781 acgaagtcat cgcaacagcg gacatgagtt acgaaagctt gcaattctat ctgtagccac
     6841 cgccgttgtc tttaagattc aggagcgtag tgcatggagt ttagtgtaaa aagcggtagc
     6901 ccggagaaac agcggagtgc ctgcatcgtc gtgggcgtct tcgaaccacg tcgcctttct
     6961 ccgattgcag aacagctcga taaaatcagc gatgggtaca tcagcgccct gctacgccgg
     7021 ggcgaactgg aaggaaaacc ggggcagaca ttgttgctgc accatgttcc gaatgtactt
     7081 tccgagcgaa ttctccttat tggttgcggc aaagaacgtg agctggatga gcgtcagtac
     7141 aagcaggtta ttcagaaaac cattaatacg ctgaatgata ctggctcgat ggaagcggtc
     7201 tgctttctga ctgaactgca cgttaaaggc cgtaacaact actggaaagt gcgtcaggct
     7261 gtcgagacag caaaagagac gctctacagt ttcgatcagt tgaaaacgaa taagagcgaa
     7321 ccgcgtcgtc cgctgcgtaa aatggtgttc aacgtgccga cccgccgtga actgaccagc
     7381 ggtgagcgcg cgatccagca cggtcaggcg attgccgccg ggataaaagc agcgaaagat
     7441 ctcggtaata tgccgccgaa tatctgtaac gccgcttatc tcgcctctca ggcgcgccag
     7501 ctggctgaca gctacagcaa gaatgtcatc acccgcgtta tcggcgaaca gcagatgaaa
     7561 gagctgggaa tgcactctta tctggcggtc ggtcagggtt cacaaaacga atcgctgatg
     7621 tcggtgattg agtacaaagg caacgcgtcg gaagatgcac gcccaatcgt gctggtgggt
     7681 aaaggtttaa ccttcgactc cggcggtatc tccatcaagc cttcagaagg catggatgag
     7741 atgaagtacg atatgtgcgg cgcggcggca gtttacggcg tgatgcgtat ggtcgcggag
     7801 ctgcaactgc cgattaacgt tattggcgtg ctggcaggct gtgaaaacat gcctggcggg
     7861 cgtgcctatc gtccgggcga tgtgttaacc accatgtccg gtcaaaccgt tgaagtgctg
     7921 aacaccgatg ccgaaggccg cctggtactg tgcgacgtgt taacttacgt tgaacgtttt
     7981 gagccggaag cagtgattga cgtggcgacg ctgaccggtg cctgcgtgat cgcgctgggt
     8041 catcatatta ctggtctgat gacgaaccat aatccgctgg cccatgaact gattgccgcg
     8101 tctgaacaat ccggtgaccg cgcatggcgc ttaccgctgg gtgacgagta tcaggaacag
     8161 ctggagtcca attttgccga tatggcgaac attggcggtc gtcctggtgg ggcgattacc
     8221 gcaggttgct tcctgtcacg ctttacccgt aagtataact gggcgcacct ggatattgca
     8281 ggaaccgcct ggcgttctgg taaagcaaaa ggcgcaaccg gtcgtccggt agcgttgctg
     8341 gcacagttcc tgttaaaccg cgctgggttt aacggcgaag agtaagtttg cgttttaccc
     8401 tcaccctaac cctctcccta agggagaggg aaccgatcga gcgcaataga aattcattgc
     8461 tcatctttca gggtgaggaa tgcagcaata tttaaatcca ccacaagaag ccccatatat
     8521 gaaaaacgca acgttctacc ttctggacaa tgacaccacc gtcgatggct taagcgccgt
     8581 tgagcaactg gtgtgtgaaa ttgccgcaga acgttggcgc agcggtaagc gcgtactcat
     8641 cgcctgtgaa gatgaaaagc aggcttaccg gctggatgaa gccctgtggg cgcgtccggc
     8701 agaaagcttt gttccgcata atttagcggg agaaggaccg cgcggcagtg cgccggtgga
     8761 gatcgcctgg ccgcaaaaac gcagcagcag cccgcgcgat atattgatta gtctgcgaac
     8821 aagctttgca gattttgcca ccgctttcac agaagtggta gacttcgttc cttatgaaga
     8881 ttctctgaaa caactggcgc gcgaacgcta taaagcctac cgcgtggctg gtttcaacct
     8941 gaatacggca acctggaaat aatggaaaag acatacaacc cacaagatat cgaacagccg
     9001 ctttacgagc actgggaaaa gcagggctac tttaagccta atggcgatga aagccaggaa
     9061 agtttctgca tcatgatccc gccgccgaac gtcaccggca gtttgcatat gggtcacgcc
     9121 ttccagcaaa ccatcatgga taccatgatc cgctatcagc gcatgcaggg caaaaacacc
     9181 ctgtggcagg tcggtactga ccacgccggg atcgctaccc agatggtcgt tgagcgcaag
     9241 attgccgcag aagaaggtaa aacccgtcac gactacggcc gcgaagcttt catcgacaaa
     9301 atctgggaat ggaaagcgga atctggcggc accattaccc gtcagatgcg ccgtctcggc
     9361 aactccgtcg actgggagcg tgaacgcttc accatggacg aaggcctgtc caatgcggtg
     9421 aaagaagttt tcgttcgtct gtataaagaa gacctgattt accgtggcaa gcgcctggta
     9481 aactgggacc cgaaactgcg taccgctatt tctgacctgg aagtggaaaa ccgcgaatcg
     9541 aaaggttcga tgtggcacat ccgctatccg ctggctgacg gcgcgaaaac cgcagacggt
     9601 aaagattatc tggtggttgc gaccactcgc ccggaaaccc tgctgggcga taccggtgta
     9661 gctgttaacc cggaagatcc gcgttacaaa gatctgattg gcaaatatgt cattctgccg
     9721 ctggttaacc gtcgtattcc gatcgttggc gacgaacacg ccgacatgga aaaaggcacc
     9781 ggctgcgtga agatcacacc ggcgcacgac tttaacgact atgaagtcgg taaacgtcac
     9841 gccctgccga tgatcaacat cctgaccttt gacggtgata tccgtgaaag cgcccaggtg
     9901 ttcgatacca aaggtaacga atctgacgtt tattccagcg agatcccggc agagttccag
     9961 aaactcgagc gttttgctgc acgtaaagca gtcgttgctg cggttgatgc actcggcctg
    10021 ctggaagaaa ttaaaccgca cgacctgacc gttccttacg gcgaccgtgg cggcgtggtt
    10081 atcgaaccga tgctgaccga ccagtggtac gtgcgtgccg atgtactggc gaaaccggca
    10141 gttgaagcgg ttgagaacgg cgacattcag ttcgtaccga agcagtacga aaacatgtac
    10201 ttctcctgga tgcgcgatat tcaggactgg tgtatctctc gtcagctgtg gtggggtcac
    10261 cgtatcccgg catggtatga cgaagcgggt aatgtttatg ttggccgcaa cgaagaagaa
    10321 gtgcgcaagg aaaataacct cggtgccaac gttgccctgc gtcaggacga agacgttctc
    10381 gacacctggt tctcttctgc actgtggacc ttctctaccc ttggctggcc ggaaaatacc
    10441 gacgccctgc gtcagttcca cccaaccagc gtgatggtat ctggtttcga catcatcttc
    10501 ttctggattg cccgcatgat catgatgacc atgcacttca tcaaagatga aaatggcaaa
    10561 ccgcaggtgc cgttccacac tgtttacatg accggtctga ttcgtgacga cgaaggtcag
    10621 aagatgtcca aatccaaggg taacgttatt gacccgctgg atatggttga cggtatttcg
    10681 ctgccagaac tgctggaaaa acgcaccggc aatatgatgc agccgcagct ggcggacaaa
    10741 atccgtaagc gcaccgagaa acagttcccg aacggcattg agccgcacgg caccgatgcc
    10801 ctgcgcttca ccctggcggc gctggcgtct accggtcgtg acatcaactg ggatatgaag
    10861 cgtctggaag gctaccgtaa cttctgtaac aagctgtgga acgccagccg ctttgtgctg
    10921 atgaacacag aaggtcagga ttgcggcttc aacggcggcg aaatgacgct atcgctggcg
    10981 gaccgctgga ttctggcgga gttcaaccag actatcaaag cgtaccgcga agcgctggac
    11041 agcttccgct tcgatatcgc cgcaggcatt ttgtatgagt tcacctggaa ccagttctgc
    11101 gactggtatc tggagctgac caagccggta atgaacggtg gaacagaagc cgaactgcgc
    11161 ggtactcgcc atacgctggt gactgtactg gaaggtctgc tgcgcctcgc gcatccgatc
    11221 attccgttca tcaccgaaac catctggcag cgtgtgaaag tactttgcgg tatcactgcc
    11281 gacaccatca tgctgcagcc gttcccgcag tatgatgcat ctcaggttga tgaagccgca
    11341 ctggccgaca ccgagtggct gaaacaggcg atcgttgctg tacgtaacat ccgtgcagaa
    11401 atgaacatcg cgccgggcaa accgctggag ctgctgctgc gtggttgcag cgcggatgca
    11461 gaacgtcgcg taaatgaaaa ccgtggcttc ctgcaaacgc tggcgcgtct ggaaagcatc
    11521 accgtgctgc ctgccgatga caaaggtccg gtttccgtta ccaagatcat cgacggcgca
    11581 gagctgctga tcccgatggc tggcctcatc aacaaagaag atgagctggc gcgcctggcg
    11641 aaagaagtgg cgaagatcga aggtgaaatc agccgcatcg agaacaagct ggcaaatgaa
    11701 ggctttgtcg cccgcgcacc ggaagcggtt atcgcgaaag agcgtgagaa gctggaaggt
    11761 tatgcagaag cgaaagccaa gctgattgaa cagcaggctg ttatcgccgc actgtaatca
    11821 acacagtgat gaaaacgaag gccggagcat gctccggcct tttttatctc ttacagaaat
    11881 ggtaaagaag gcataactcc ccgagaaata ctggctaaga agcctttatc tggttgttct
    11941 tctttattga ttaaaggaag gtcatccaaa tcgcccagca aatacgtatt ttttgcctgc
    12001 cagtcaatca tccatatttt cagcagtggg taagccagac cacccgtaat cccggatatc
    12061 accaccaacg cacacatgcg ataaagcata ccgtggtacg ttaaagttga gcgaaaacga
    12121 atacgcccat cattcagtga caggttgctc ataaaatgat ttcgcaacga caccgttaaa
    12181 taactggtgc taacagcaat cccaaaataa taaattaact gcgcgattat cattttacgt
    12241 tgcatttcca taaattgctg taaattctca atatcatcat ttgcataccc agaactatca
    12301 tactcattta atatttgatc gaagataata taaccagcaa cagcgagaaa aggtaataaa
    12361 gctaaaaatg cgaggatggc atattttata caatacgcag tatctaattt caccttgaaa
    12421 cgatgtatac cgaaactggt attgctccag agaaaactca ttaccaggct atataaagta
    12481 ccattaaaaa taccaatgga aacaatacca actattgcca tcagaaccac agatataata
    12541 acactacttg aactattggc atgtagcatc tttgtcgaga taaagaaaac agtccccatc
    12601 ccaatggcca ttaaaatcgg caagaaaaag gttacccacc agaacccttt caaagagcaa
    12661 ttaaaactaa atcttacgcc gttgagactg gtcattaagg cctgataacg cagtcctttt
    12721 gctgccataa atataagcaa aaccaacagt aacaaagtca aaacacagcc aacaagcggc
    12781 atatctgctg acactgtcat taagattgcg aaatagaaaa aaacaaaaac aagacaacta
    12841 acaaaaacat tcccaccggt aattccataa gaaaatcgtt gtccgttaac ttccatatta
    12901 gcataaagat aacgcttaca tttcattaat gcccatggta aatagatacc tagcgttata
    12961 atcgttaaca acacattcac cacacaaata agaaaatatc tctcacctgt accatgaaaa
    13021 acaaaagaat gggacggaac atccatttca ttaataactt gagccataaa aatcccctga
    13081 attaaaaaat ccattgatat aatttataga tacagacagt aaaatgttct gccacgttac
    13141 aatactctta tcagtaatag gctggcaaaa actttattgc tttataagac gcttgcgcac
    13201 gtaagaagag agtggtatta aacaaatgta taattacttt atttttattg ggttaaacta
    13261 tgaataacat tgcgccgcaa tcacctgtaa tgcgtcgcct gacgctgcag gataatcctg
    13321 ctatcgccag cgtcatccgt caggtttacg cagagtatgg tcttaccgcc gataaaggct
    13381 acaccgttgc cgatccgaat ctcgacgaac tgtatcaagt gtacagccag cctggccatg
    13441 cttattgggt ggttgaatac aatggcgaag tggtcggcgg cggcgggatt gcgccattaa
    13501 caggtagtga agcggacatt tgcgaactgc agaaaatgta ctttcttccc gctattcgcg
    13561 gtaaagggct agcgaaaaaa ctggccttaa tggcgatgga gcaggcacga gagatgggct
    13621 tcaaacgctg ctatctggaa acgaccgctt ttttaaagga agccattgcg ctttatgagc
    13681 atttgggctt tgagcatatc gactatgcgc ttggctgcac gggccatgtc gattgtgaag
    13741 tgcggatgct acgcgaactc taaaaaaatg cccgctagta tgtgctatcg ggcattttaa
    13801 tcaattaatt agtggcgaac cccgtcatcg tcttcatcga caaaatcttc atcgtcgcca
    13861 tcttcgccgt taggatcttc aaagtaagtg ccccaaccgt cgtactcaac gtcaaatttc
    13921 tccgccagcg tcatcagttg ttcaacctgg gcatcgatca gatcggcatt caacgcgcac
    13981 tcgctgagga tgtcgcagca aatcacgata tcaccatctt caacttccag ctcttctgga
    14041 tcggtcactt cgtaaccgag tttaaacgct tcaactgccg ctttttccag agtttccaga
    14101 tcgtctgcgg aaagatggtg ttcgatggtg tacagcgcgt ccggatcgct gccatcttcc
    14161 agtaattctt caataatcaa acgtgtttct tcacgctgtt cttccagttg ttccgggttt
    14221 gccatggctc attcctctta atgtcctgcc gatactctta ttgtcacaca ccactgacat
    14281 tgcctccacc tttgtgacaa agatttatgc tttagacttg caaatgaata ataatacaca
    14341 taaattgagt tttaattcat tgaggcgtta gccacaggag ggatctatgt ccgggtttta
    14401 tcataagcat ttcctgaaat tactcgattt cacgccagct gaactcaaca gcctgctgca
    14461 gttagccgcg aagctgaaag ctgataagaa aagcggtaaa gaagaagcca aactcactgg
    14521 taaaaacatc gcgctcatct tcgaaaaaga ctcgactcgt acccgatgct ctttcgaagt
    14581 tgccgcatat gaccaggggg ctcgcgttac ttatcttggc ccaagcggta gtcagattgg
    14641 tcataaagag tcgattaaag acactgcccg cgtgcttggt cgcatgtatg acggtattca
    14701 gtatcgcggc tatggtcagg agattgtcga aacactggcg gaatacgctg gcgtgccggt
    14761 atggaacggc ctgaccaatg agtttcaccc cacgcagttg ctggcggatc tcctcaccat
    14821 gcaggagcat ttacccggta aagcattcaa cgaaatgacg ctggtctatg caggtgacgc
    14881 acgtaacaac atgggcaatt cgatgctcga agctgcggcg cttaccggtc tggatttgcg
    14941 tctggtcgcg ccacaggcat gctggccaga agctgcgctg gttacggaat gccgcgccct
    15001 ggcacaacaa aatggcggga atattacgct gacggaagat gtagctaaag gagttgaagg
    15061 tgctgacttt atctataccg atgtatgggt gtcgatgggt gaagcaaaag aaaaatgggc
    15121 tgaacgcatt gcattactgc gtgattatca ggtgaacagc aagatgatgc agttgaccgg
    15181 taacccggag gtcaaattcc tccattgcct gcccgcgttt catgacgacc aaacgacgct
    15241 tggcaaaaaa atggcggaag agtttggcct acatggcgga atggaagtga ctgatgaggt
    15301 cttcgaatct gccgccagca ttgtatttga tcaggcggaa aaccgcatgc ataccatcaa
    15361 agcggtgatg gtcgcgacgc tcagtaaatt aaataattaa tatcgctata aa
//
LOCUS       DAEBXN010000095        15371 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.095, whole genome shotgun sequence.
ACCESSION   DAEBXN010000095 DAEBXN010000000
VERSION     DAEBXN010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15371
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.095"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            95..1291
                     /locus_tag="KJL99_003118"
                     /pseudo
     CDS             95..1291
                     /locus_tag="KJL99_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611482.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1374..1631
                     /locus_tag="KJL99_003119"
     CDS             1374..1631
                     /locus_tag="KJL99_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4437756.1"
                     /translation="MTLVNDTGFDPVFSGSIAESWRQQPCTPSYCCDWEAATMLRAFP
                     LAKKGEGRARLPSLYASFGKLGETPTHEDIIDNNRSINWPV"
     gene            complement(2189..2956)
                     /gene="fecE"
                     /locus_tag="KJL99_003120"
     CDS             complement(2189..2956)
                     /gene="fecE"
                     /locus_tag="KJL99_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HBD4437757.1"
                     /translation="MTLRTENLTVSYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(2957..3913)
                     /gene="fecD"
                     /locus_tag="KJL99_003121"
     CDS             complement(2957..3913)
                     /gene="fecD"
                     /locus_tag="KJL99_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HBD4437758.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPHTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            complement(3910..4908)
                     /gene="fecC"
                     /locus_tag="KJL99_003122"
     CDS             complement(3910..4908)
                     /gene="fecC"
                     /locus_tag="KJL99_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HBD4437759.1"
                     /translation="MTAIKHPMLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            complement(4905..5807)
                     /gene="fecB"
                     /locus_tag="KJL99_003123"
     CDS             complement(4905..5807)
                     /gene="fecB"
                     /locus_tag="KJL99_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HBD4437760.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAAMVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIAPVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGSSMPS
                     IGLEQLLAVNPTWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            complement(5852..8176)
                     /gene="fecA"
                     /locus_tag="KJL99_003124"
     CDS             complement(5852..8176)
                     /gene="fecA"
                     /locus_tag="KJL99_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate transport protein FecA"
                     /protein_id="HBD4437761.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLASRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWTF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVAYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGSLKF"
     gene            complement(8263..9216)
                     /gene="fecR"
                     /locus_tag="KJL99_003125"
     CDS             complement(8263..9216)
                     /gene="fecR"
                     /locus_tag="KJL99_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fec operon regulator FecR"
                     /protein_id="HBD4437762.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            complement(9213..9734)
                     /gene="fecI"
                     /locus_tag="KJL99_003126"
     CDS             complement(9213..9734)
                     /gene="fecI"
                     /locus_tag="KJL99_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HBD4437763.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            10032..10214
                     /locus_tag="KJL99_003127"
                     /pseudo
     CDS             10032..10214
                     /locus_tag="KJL99_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767718.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            10208..10312
                     /locus_tag="KJL99_003128"
                     /pseudo
     CDS             10208..10312
                     /locus_tag="KJL99_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            10652..10909
                     /locus_tag="KJL99_003129"
     CDS             10652..10909
                     /locus_tag="KJL99_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm development YmgB/AriR family protein"
                     /protein_id="HBD4437764.1"
                     /translation="MHSDYYNMVFGEKLANILYEANSQFFYERNVIEEAVNALFCERE
                     IINNKNIIKKLMFFLSDVNHTKKDVVQSALNIIIDITSGDI"
     gene            complement(11229..11360)
                     /locus_tag="KJL99_003130"
                     /pseudo
     CDS             complement(11229..11360)
                     /locus_tag="KJL99_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            11642..13000
                     /locus_tag="KJL99_003131"
     CDS             11642..13000
                     /locus_tag="KJL99_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HBD4437765.1"
                     /translation="MKRKIIPVLIGCTLSFSALAAQPTAERYVVSFPEGTHVNYAGAF
                     ASAFPNGLPVGIGSGLLFTGKQGDALTFATITDRGPNADSPKEGKNETKIFVTPDFAP
                     LLMTIRVQNGKAEAIDPRPLHDDKGAINGLPLASDVIGSTNEVAFSDTLHRLKGDNRG
                     LDTEGITPDGKGGYWLCDEYGPFLINIDSKGKILAIHGPQAAEGEKAIAGGLPNILKW
                     RQANRGFEGLTRMPDGRIIVAVQSTLDIDAKSKKKALFTRLVSFDPASGKTAMYGYPI
                     DSAAYSKNSDAKIGDIVALDNQHILLIEQGRDKNNRMRNLIYEVDLNKASDLSGFDKP
                     GEYPEFDDEKTLSQRGITLAQKTQVVDLRSLGWQQEKAEGLALIDSKTLAVANDNDFG
                     VKVAMQHPVEGKTFKDYRVNAEGKLTLDDKQVETTLRVKPLEKPESDSELWIVTLPEA
                     LK"
     gene            complement(13239..14624)
                     /locus_tag="KJL99_003132"
     CDS             complement(13239..14624)
                     /locus_tag="KJL99_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HBD4437766.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQC
                     DTIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYCQSEIYGRQGVELSRSLLSG
                     WVDACCRLLSPLEEALHGYVMTDGKLHADDTPVQVLLPGNKKTKTGRLWAYVRDDRNA
                     GSALAPAVWFAYSPDRKSIHPQTHLACFSGVLQADAYAGFNELYRNGGITEAACWAHA
                     RRKIHDVHVRIPSALTEEALEQIGQLYAIEADIRGMPAEQRLAERQRKTKPLLKSLES
                     WLREKMKTLFFGSGHGGERGALLYSLIGTCKLNDVDPESYLRHVLGVIADWPVNRVSE
                     LLPWRIALPAE"
     gene            complement(14674..15021)
                     /gene="tnpB"
                     /locus_tag="KJL99_003133"
     CDS             complement(14674..15021)
                     /gene="tnpB"
                     /locus_tag="KJL99_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HBD4437767.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNSFNGLAAKVQTALKDDPMSGHIF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            complement(15018..>15371)
                     /locus_tag="KJL99_003134"
     CDS             complement(15018..>15371)
                     /locus_tag="KJL99_003134"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBD4437768.1"
                     /translation="RKGCPNYPPEFKQLLVAASCEPGISISKLALENGINANLLFKWR
                     QQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEDPETLSISCEVTFRHGTLRFNGN
                     VSEKLLTLLIQELKR"
BASE COUNT         3623 a         4081 c         4205 g         3462 t
ORIGIN      
        1 ttataaacaa taatatatta tcatatcttt taaaaacggc tcactaagaa ccttgaggtg
       61 gcacactaaa tttagctacc tgaacagagg tgatatgctc acctcagaac aacacaggtg
      121 ccataatgaa aaaagaaatt tcagcgcaga gtttaaacgc gaatccgctc aactggtcgt
      181 tgaccagaaa tacaccgtgg cagatgcagc cagcgctatg gatgtcggcc tttccacaat
      241 gacgcgatgg gtgaaacaat tacgtgatga gcggcaggga aaaacaccaa aagcctcccc
      301 cattaccccg gaacaaattg aaatccgtga gctcaggaaa aagctacaac gtattgaaat
      361 ggaaaatgaa atattaaaaa ggctaccgcg ctcttgatgt cagactccct gaacagtttt
      421 cgataatcgg gaaactcagg gcgcgttatc ctgtggccac tctctgccat gtgttcgggg
      481 tccatcgcag cagctacaaa tactggaaaa accgtcctga aaaaccagac ggcagacggg
      541 ctgtattacg cagccaggta cttgaactgc atggcatcag ccacggctct gccggagcaa
      601 gaagcatcgc cacaatggca acccagagag gctaccagat ggggcgctgg cttgctggca
      661 gactcatgaa agagctgggg ctggtcagtt gccagcagcc gactcaccgg tataagcgtg
      721 gcggtcatga gcacgttgct atcccgaatc atcttgagcg acagttcgcc gtaacggaac
      781 caaatcaggt gtggtgcggt gatgtgacct atatctggac gggtaagcgc tgggcgtacc
      841 tcgccgttgt tctcgacctg ttcgcaagaa aaccagtggg ctgggccatc tcgttctcgc
      901 cggacagcag gcttaccatg aaagcactgg aaatggcatg ggaaacccgt ggtaagcccg
      961 tcggggtgat gttccaagcg atcaaggcag tcattatacg agcaggcagt tccggcagtt
     1021 actgtggcga taccagatca gacagagtat gagccggcgt ggaaactgct gggataacag
     1081 cccaatggag cgcttcttca ggagtctgaa gaactaatgg gtgccagcga cgggctatgt
     1141 aagcttcagc gatgcagctc acgcaataac ggactatatc gttggatatt acagcgcact
     1201 aagaccgcac gaatataatg gtgggttacc accaaacgaa tcagaaaacc gatactggaa
     1261 aaactctaac gcggaggcca gttttagttg accacaacag actacctgaa gggagccgcg
     1321 gtcgcctagc agttgcagta gcaggagatc atccagccgc agtacaggtc acgatgactc
     1381 tggttaatga taccggcttt gaccccgtat tttccggctc tatcgctgaa tcatggcgtc
     1441 agcagccgtg cacaccatcc tattgttgtg actgggaggc tgccaccatg cttcgcgctt
     1501 tccctctggc gaaaaaggga gaaggacggg cccgtctgcc ttcactttat gccagcttcg
     1561 gtaagctggg tgagacaccg actcatgaag atatcattga taacaatcga tccatcaact
     1621 ggcctgtata acgtggctgc cggtgattaa gaaagctgca cctacctaag tagtagcaaa
     1681 cgcacacttt ttagaaaaat cgatggtcag aaactggatt agcaattccg ttccagggtt
     1741 gcttttgatt tacgttggcg tctgatcatt gatttatcct caaaagccca acctcattgg
     1801 taatgaacca gctccgtgaa tgtccgctct ggcacagagc gaaatttttt gatctccccc
     1861 cctgaaatct aaacttagtc atgtcacgtt tttgggtttc taaaatttta acttcgcgtt
     1921 tttcgttgcc gtaagggtta tacagaaatg tccgttaagc agagttcaaa attgattgcc
     1981 gtgatcacga ctggtttgaa agccgcgccc aagcctgtac agctctggtt tgcgttgatt
     2041 atgaacctgt cagcctaaag caagcggatg gacgatgagt attggtaatc tttcagagtc
     2101 cggaaaagtt cagccccagt ctgaacaggc ttgctggcgc cagtccagtt tcattcagtc
     2161 gtggtttggt tcttacggcc tgtgcaatct acctcattag gcacatcggc ctgccagata
     2221 ccggctcggg gtgtatttcc gcttccacgc tgaatactgt tctcagcaat cctggggtca
     2281 tcacctcttc tggtgtgcct tgcgccataa catgtccgtt tgccattacc accagttgat
     2341 cgcagtaccg gctagcctga ttaaggtcgt gcagcacagc gaccaccgtt ttcccctgag
     2401 tccggagttc gcccatcaac cgcatcaggt ccacctggtg attgatatca agataggtgg
     2461 ttggctcatc aagtaataca acgggcgtat tctgggccag gaccatcgcc agaaatgcgc
     2521 gctggcgctg accgccggaa agctcggtta accgacgaac ggcaagatga ttgatccggg
     2581 tctggttcat ggcgacatta actcgtgcat tgtcttcagc ggagagacgc ccccagagtg
     2641 acagccaggg attacgacca tacgaaacca gctcctggac tgtgatcccc tctggcgtta
     2701 aatggtgctg aggcagcagc gaaagcctgc gggccaactg gcgtgatgag agcatattta
     2761 tgggattatc gccgagaaat acggtgccag actgcggcat taaaagccgc gaaaaacagt
     2821 ttaacagcgt cgatttcccg caaccgttag ggccgatcag ggcggtgatc ttccccgttg
     2881 gcagtgagag tgaaacgtcg ttaagtacct tgtctgtccc gtaactgacc gtcagatttt
     2941 cagttcgtaa agtcatttat cgcattctca caagcaacca gacaaaccac ggcgcaccga
     3001 taatggcggt cagcacgcca accgggagct ccagtggggg atgaataatt ctcgccagca
     3061 gatcggcaac caccaacagc aacgcacctg tcagggctga aacaggcagc agtctgcggt
     3121 gacgtccacc ggtgatgcta cgcatcatat gcggcaccac gagaccaata aagctaatcg
     3181 ggccgcaggc ggccacgccg gtagatgtca tggcgacagc tagtaacaaa gcccagaatc
     3241 gggtatgggg caccgacaca ccgagcgtgg tggcgcgcgc atcgccgagt gcaaggaggt
     3301 cgagatcgcg gcaaaaactc aggctcagcg gcagaaataa aatcatcagc gggatggcaa
     3361 tcttcacaaa gctccagtca cggccccata agctgccggt cagccacagc agggcgttgt
     3421 tcacatcctg cgggcgcgag agcatcagat aatccgtcag gctggcccag catgcagaaa
     3481 gcgccacgcc ggtgagcgcc agcttcatcg gctggtgggt ctttgccagc atcttcagta
     3541 atatcaaccc cgccatgccg cccgcaaagg ccagcagcgg cagcaccatc acgggcagtg
     3601 acggcataag aagtagagcc cccacagagg ccaggctggc ggcatggtta acgccgagaa
     3661 tatccggtga tgccagaggg ttgcgcacaa tcccctgtat cagcacgccc gccacggcga
     3721 gggctgcacc gacaaacagt gccagcagca agcgcggcag tcggtactcc atcaatacat
     3781 aataatgctc gcgtccggcc tgccagtcgg tcagcagcgc gcgccacggc acggggatca
     3841 ctcccatatg gagtgataac agcgcacagc ccgccagggc aagggtgatg aaaataacca
     3901 gcgcaatttt catcctcgcc tcctcacaag ccagacaaag caagggctgc caatcagcgc
     3961 cagcactgcg cctgcgggca gatctccggg gaaggccagc gcgcgtgcga gtacatctgc
     4021 cagcagcatc agcgtggccc ccagcagcat gctcactggc agtacgttgc gctgatcgaa
     4081 gcctgcccag aagcgcgcca gatgtggcac cagcagaccg ataaacgcca ccggacctgc
     4141 cacgctgacg cacgcaccaa ccagaagcag cactaacata ttgatgacca aacgtagcct
     4201 cgtcaggttc actcccagcg tatgggcggt gctgtcgctg aggttgagca ggttcagttg
     4261 attcgccagc agtaacacga cagggactgc agtgaccacc accggcaaga gctgccagac
     4321 atcctgccag cgggcgtggg acactccgcc tgccagccag taaaagatgc cgtaagcatg
     4381 atcttcggcc agcagcaggg tgatgcgggt aaggcccata caaaaggccg acagcgcgat
     4441 acccgcgagg atcagtttgt ttctgtcatg ggtatgacga aatccgcctc ctgcggtcat
     4501 gaccagcagc cagctcacgc cgcccccgca tgccgcgatg aacgacagag aatagcctgc
     4561 aatcggcgtc ggactcagcg cgctggtaag cgccatagcc agcgccgcgc cgctgttaat
     4621 gccgagcagt gaaggagagg ccattgggtt gtgggtcagg gtttgcagca gcgtgcccgc
     4681 gagcgccagg cttgcgccga tcagaacggc gaccaggctt cgtggcaaac gaaggttttg
     4741 caccagcgct tctggtagcg ttggcgtgtg tccaggcagc agggcgcggg ttgcatctgc
     4801 tccggaaaca ggaatggccg agtagcaaaa cagactcagc cagaaaataa taataagtgc
     4861 tgcaacggga agcccccaca gcagcatcgg gtgttttatc gcggtcattt cacaacggta
     4921 agcggctgat ggtggaagat ttttaccgtg tcagcggcaa tacgctctgc agcaaaaata
     4981 ccgcgcatcc gcgcccaggt gttactgtcg accgaagcaa cctgctgctt ctgcgcggcg
     5041 gttaacatct gccagagcgg atcttgttgc cagcgtttaa caatgctctc ttcgcgatag
     5101 tgggcaacca gcagccaggt aggattgacc gccagcagtt gctccaggcc gatggacggc
     5161 atggacgaac ccgccatcgc agcgggaacg ttcagcccca gagaggccag cacgctgccg
     5221 gtccaggtct cctgagtatg caggttgaat tgctgttcgc gtgatgtgcc aaaggccacg
     5281 cgtgtccctt tgggaagctg gctggcccac tgcgccatcc tctctttatg ttgttccaga
     5341 cgtgcctgca tctctcgctt tttacccacc atttcgccga tgatagccgc agattgcaaa
     5401 ttttcagcgt aggtttcgtt gcgggactta agcagcagta ccggcgcgat ttgctgcaag
     5461 gcgatgtaaa cccccgcatg gcgactgctg tcggcaatga tcaggtctgg tttcagagcg
     5521 gcaatggctt ccaggctcgg ctgcgcgcgc gttccgacgg actgccacgg tttcaggtgc
     5581 gcacgcactt cgggcaggat gcgttttgca tcgttatcgt cggcaatacc gatcgggctg
     5641 acgtccacgg cggccagcgc atcggcgaac gagagttcca gcaccacaat ccgttgtggc
     5701 gttttttcga gtgtaaacgt gccgtgttcg tcctgaacca tggcggcaaa ggcgtggctg
     5761 atcaccagca gcaggcctgc aaaaagaaaa cggataaatg ccaacataat cacattccag
     5821 ctaaaagccc ggcaagccgg gcgttaacac atcagaactt caacgacccc tgcatataca
     5881 gcgtgcgcgg ctggcctgca tagatgcctt tgttgttgtc gtcataagag cggatgaagt
     5941 agtcctggtc gaagatgttt ttcacaccga atgccaggtt cagatctgcc atctgcgggc
     6001 caaagtcata cgccacgcgt gcaccccaga gcatgaagcc gggaatgcgg ccggtactgc
     6061 cgtcggcgct ctctttcacc gtattggcgt tatccgcaaa ctggctggac tggaaatcgc
     6121 tgttcagatt gaacgtccag tttcctggct tgtagtccac gcccagcgtg cctttatgtt
     6181 tcggggagaa tggtaccaga ttgccgtagg tgtcgccttt ctcgcggatt tccgcgttca
     6241 catacgcata gctggcgtag atggaaacgt tatcaagcgt tggcgttagc gtacccagat
     6301 cgtaacgtgc ctgcgtttcc agcccggtat ggcgcgtttt gccacgtgca gtgacggtgt
     6361 cgttggtctg gttggagtcg tactgattgt taaagttaat caggaacagc cccatttccg
     6421 ccgtcagcgc gccgtcgtcg tagcgggtac cgagttccca ggttcgcgct ttttccggtt
     6481 ccacattgcc gctttgcaca gccttgccaa tctggctgta ctgtacggtg ccgaacgagc
     6541 cttcagtgtt tgcataaaga ttccagctgt cagtcaggtg atagagcacg ttcaacgccg
     6601 gaagcggtgc gttatagctc acttcttcgt gcgtgcctgt gatggcgttg ttctggtatg
     6661 actcgatatg ttcgaaacgc atacccggcg tgatggtcca gttgccgatg tcgattttgt
     6721 catccagata ccaggcgtgc gcctcggtgc cggaacgcgt atcgcggtcg taagggcttg
     6781 agccggacgg caactgcccg ctgctggtgg cggtgtagta acgcatttca tgcgttgatt
     6841 cattcacata gcgatagccc acgcccactt cgtgcgcgga agggccgatc ataaagatct
     6901 ggctgtagcg tggctcaata ccgcgcaccc agtagttacg cggcgagagg gtgatgcgtt
     6961 tgccttgctc caggtagccg ctgcgcaggg tttgggtgta gaacccctga atgttgaatt
     7021 tatgctggct gtctggctgg aactggtagc ccaggctcgc cagcttgcga cgaccccaga
     7081 agcggtcata cgggcgggtg gattgccagc gatcggcgtc gtaatccgcg cgagacaggc
     7141 caccgggcat gtcggcttca ccgtcgtaat attgcagcag gctgttgaag gtgtgcacct
     7201 catccggcgc atatttgctt ttcagcatca ggtcgtcgat gcgggtggcg ctgtgctcgc
     7261 gccagtcact gccgcgcgtg ccggagtaga gcagcgcggt gccaaaaccg ttgtccgctg
     7321 tgccgcccac catcaggttg tgcgtctctt tcgggttgtt ttgtgaagag gttgggctga
     7381 gctgaccttc cacgcccgcc tcgataccaa agtcctgcgg aatggcacgg gtaacaaagt
     7441 tcaccacgcc gcccacgctc tgcggtccgt aacgcaccgc accaccaccg cgtaccacgt
     7501 caatggcatc catgttgccg agcgaaacgg gagccagtga aagctgcggc tgaccgtaag
     7561 gggcgaaggg gacggggatg ccgtccatca ggacggtcga gcggctggcg aggcgcgggt
     7621 tcaggccccg gatgccaaag ttcatcgcca ggtcgtggct gccggtgccg ttgttttccg
     7681 gcgcgctgac gccagggatg cggttaagta cctcacgcat ggtggttgcg ccggttttgg
     7741 cgaaatcctc acggcggatc acgtcacgcg cgccagcatg ttcaaatacg tcgttttcac
     7801 gcgcatcacc cagccagtcg ccgaccacgg tcagggcatc ttcttttggt gcgggcgcgg
     7861 gctccagcgt ccagctgtta tttcccagcg gttttacctg cagtccgctg ccgtccagca
     7921 gttgttgcag gccgctctcg acgtcgtaat cgccgtgcag gccgttgctc tgcttgccgc
     7981 gcgtcaggct ggcgtcaacc gagagggtaa atccgctgtg tgcggcatac tgattgagcg
     8041 ctttatcgag cgatcccggt gcgatattaa cctgtgcagc aaaagcggaa aacgagagac
     8101 cggccagcgg cagcaggctc aggcgaatgg cgttaaccaa aggtgttgtt ttacgaaaaa
     8161 cgcgtaacgg cgtcatacct tccccatcat catttttgtt gtgttcagct atgagtcgaa
     8221 cgagaagggt aaaaaggaca atcgaaataa gaattatttt ccttacagtg gtgaaatgtt
     8281 tatccagtac cgcgtaatag actgaatttt aacgggaagc gtttgcgcga taacgttcag
     8341 gatcgcatcg gtatttttca gcgggaacgt cccgctcagg cgcagcccgg caacggcggg
     8401 atcgcagcgc agcacgccgt tgcggtaacg ggttagcgtg gctatcacct cacccagcgg
     8461 tttatcgctg aagctcagga tgtccttcgt ccagcttgta ctctcgtcat ccagcggttt
     8521 cactgcgcca aactcagagg cgctgaactg caggctttca ccagcgttca cgatgcgttt
     8581 ttgcgcgggg gcactggcga gaagcacttc cacagcgtgc tgctgaacgt caagctgcgt
     8641 gaaattatcc tgctggcgga cggtaaattc tgtccctaaa gcagtgagct ggccctgacg
     8701 ggtcaggaca cggaaggggc gttgcagggc atctttcgcg gtagtaatgg cgatttcacc
     8761 gtaccagagc cggacggtgc gctgatgcgc atcaaaacgc acatccgccg cgctttgggt
     8821 attcagcgtg agcagggagc catcttccag ttgctgacgg ctgactgtgc ctttggcggt
     8881 gcggtaatct gcccgcagac cttcgccggt ttccgactgc cagagctgcc agcctccgcc
     8941 agcgccgagc aacagcagta atcctttcat cacgtgacgg cgggtgaggc gggtatcgtg
     9001 caacgcccgg ctggcaacgt cgccaggcac accgccaagc tggttgcgca ggttttcaac
     9061 ctgctgccag gcccactggt tatcctgatc ctgttcatac cactgttgcc agcgcgcttc
     9121 ctgttgtggg ctgacgcgct cgccgcttag cacggcatac cagtgggaag ctgaacgcag
     9181 cgcctgacgg cgggaatcgg ttaacaaagg attcataacc catactccag acggaacagc
     9241 aggcagtgct cgacggcttt cgccacgtat tttttcacgg agctgatgga aacaccgagt
     9301 ttgtgcgcaa tctcgctgta tgtcagacca tccagttgcg aaagcagaaa cgcttcacgt
     9361 gttttgccgt ttagcccgtc cagcatgctg tcgaggagtt gtagggtctc gagttggctt
     9421 tcgcgttcct caggtgaagg cgctcccccc tccggcataa gcgccagcat ctccagatac
     9481 gctttttcca gcgcgtttcg gcgaaacagg tccaccatca cgcgtttggc gatagtgcag
     9541 aggaaggagc gaggatcgcg gatcgtcgag agcgtttcgc tgaccattac ccgcaaaaaa
     9601 gtgtcctggg caatgtcatc tgcatcaaaa gcagactgga gtttgcgcgt cagccagctt
     9661 ttcaaccagc cgtgatgtgt gccataaagc gactcgaacg ttaaggaagc tgtggtagtg
     9721 gcgcggtcag acatgcggag tgcatcaaaa gttaattatc acgtagtcat attaatatga
     9781 gaatggttat cattacaatt ggaaataaaa ttgtttccaa tagacatttt taacatgttg
     9841 tttttctaag tgttataagg taggtataaa atgggatgga gcctctgctt ctgacatgtg
     9901 tcggtcagaa tgactcatga tgtggtctgc tattattgac atcctcactg ccctaaagga
     9961 tggggatttc ggtaatgatc ttacccagca atagtggaca cgcggctaag tgagtaaact
    10021 ctcagtcaga ggtgactcac aagacaaaac agtttcaacc agtaaaaaaa cgtaaacacc
    10081 attcgcatga atttcgtagt taagatctga agcttgcaga atgcatcggt gttactgccg
    10141 gagtccgtga acttagtctg catgaatcac cacagcttta catctgtcgc agtaaacagc
    10201 aaaatcagca aacttactta attatatcga gtgcgattac aatcgctggc gatggtacag
    10261 ttggtgtggc ggtctcagtc cggaacaatt tgaaaatcat aatctcactt aaaacggcat
    10321 ccattttaca aaagatggcc gtctgtgtat ttgtaatgaa taccttctac atgtttagag
    10381 tgcataatcc acgacaaggc gatatcctaa aaattagcta gcaggcaact ttgatggcat
    10441 agataatgtt tttagtaaaa aagagttgac tgaatataat acagcctctt cctacataag
    10501 aaggaatgtg ctcacaaaaa aaaaccatta gtctgcttcc tgttcttaca ataatcagta
    10561 ttatgtattt tatttatata gcaaaatatg cttgttacac ctgtcataat taggcatggt
    10621 aaaattattg actgaaccct gggggagaaa gatgcacagt gattattata atatggtttt
    10681 tggtgaaaaa ttggccaata ttttatatga agctaactct caattttttt acgagagaaa
    10741 tgtcatagaa gaggcagtca acgctttatt ctgtgagcgt gaaataataa acaacaagaa
    10801 cataataaag aagctgatgt tctttttgtc agatgttaat cacaccaaga aagatgttgt
    10861 tcagagcgca cttaatatta tcattgacat tacatcaggt gatatttgag attttacata
    10921 aaagataatc tctcaaggag tcttatctga aaacgaaata tatagtccct ctcgttttgt
    10981 aatatcgatt ggctttgttg aatcggttac gtaccccgga gccggtcata ttttcaggta
    11041 atacacacgc tttcagacat ttcggctttc gtcatttttg tatcatgggc atagcttctg
    11101 ctacctgacc atcataatca cgcagcttca gtgaagcagt gaccacctgt ttaacccggt
    11161 gtatcgttat gatcttatcc agcaattgaa ccgccccggg tttcctggag agtgttttat
    11221 ctgtgaactc aggctgccag atcatcgttt ccgatggaag cataataagc tttttctgct
    11281 tctgccggtg ggatatggcc ctgcctttcc agcaatcgtc gattgttata ccagtccacc
    11341 cacgtgagtg tggtcagctc atcatactga cacgtgcatt acccctaccg cgataatgca
    11401 accgtacaat atagggcgtt tcctaaacgc agccctcatc actccaatcc aacaagacac
    11461 agcatttttg atcacgaaga aagacaaata atttccgtaa atgtttccgt tgttacaaat
    11521 aacaacagtg ttttacattt ctttggcctc aggcccgcgc atgttgccat tcgatgtcat
    11581 tttcctgact cgtttcttct ttatggtgta gtgtgatctt cactttatag atgctaacaa
    11641 tatgaaaaga aaaattattc ccgtgctaat cggttgtacg ctctctttct ctgccctggc
    11701 ggcacaacct acggctgagc gctatgtcgt cagttttcca gaaggcacgc acgtgaacta
    11761 cgccggcgca tttgccagtg cattcccgaa cggccttccg gttgggatag gttctggcct
    11821 gctgttcacc ggtaaacagg gcgatgccct gacattcgca accattaccg atcgcggtcc
    11881 gaacgcggat tcaccgaaag aggggaaaaa cgaaactaaa attttcgtta cgccagattt
    11941 cgcgccgctg ctgatgacga ttcgtgtaca aaacggcaaa gcggaggcca tcgatccccg
    12001 tcctctgcat gacgataaag gcgcgatcaa cggtctgccg ctggcaagcg acgtgattgg
    12061 ctccaccaat gaagtggcct tcagcgacac gttacataga ctgaaggggg ataatcgcgg
    12121 gctggatacg gaagggatca ctccggatgg taaaggaggt tactggctgt gcgatgagta
    12181 tggcccgttc ctgattaaca tcgacagtaa agggaaaatc ctggcaatcc acggtccgca
    12241 ggcggcggaa ggggaaaaag cgattgcggg cggtttgcca aatatcctca aatggcgtca
    12301 ggcgaaccgc ggctttgaag gcctcacccg tatgccggac gggcgtatta ttgtcgccgt
    12361 gcaaagtacg ctggatatcg acgctaagag caagaaaaag gcgctgttta cgcgtctggt
    12421 gagcttcgat ccggcaagcg gaaaaaccgc gatgtacggt tatcctatcg acagcgcggc
    12481 ctacagcaaa aacagcgacg ctaaaatcgg agacatcgtg gcgctcgata accagcacat
    12541 cctgctgatc gaacagggaa gagataaaaa caacagaatg cgtaacctga tttacgaggt
    12601 cgatctcaat aaggcgagcg acctgtcagg cttcgacaag ccgggtgagt atccggagtt
    12661 tgatgatgag aaaaccctgt cacagcgcgg cattaccctt gcgcagaaaa cgcaggttgt
    12721 tgatctgcgc tccttgggct ggcagcagga gaaggctgaa gggttggcgc tgatcgacag
    12781 caaaacgctg gcggtggcga acgataacga ctttggcgtg aaggtggcga tgcaacatcc
    12841 ggtcgagggc aagacattca aggattatcg ggtgaacgcg gaaggaaagc tgacgcttga
    12901 cgacaagcag gttgaaacga ccctcagagt gaagccactg gagaaaccgg agtctgacag
    12961 tgaactgtgg attgtgacgc tgccggaagc gttgaaataa gtttataagg tacggacagc
    13021 agtatcgctt agtttttcaa attcatgtat gtgaacaata ttggggttcc ggcgtagacc
    13081 cccaatgaaa gcgtcaccac gtgaaaacat gcctgcacgg tttacacgtc atcccatggt
    13141 ggcagccggt taactttcct gcgccaccag tggaaaaagg ccataagcag tccgaacaaa
    13201 agtaagcgta cagcgagggc cgtattgacg gggatgtgtt attcagctgg cagtgctatg
    13261 cgccacggaa gcagttcgct gacccggttg accggccagt ctgctatgac gccaagcaca
    13321 tggcgaaggt agctttctgg atccacgtca ttcagtttgc acgtcccgat caggctgtac
    13381 agtagcgctc cccgctcacc accatggcca gagccgaaga acagggtctt catcttttca
    13441 cgcaaccagc tttccaggga tttcaacagc ggtttcgttt ttcgctgacg ttcagcaagc
    13501 cgctgctctg ccggcattcc ccttatatcc gcctctatgg cgtacaactg accgatctgc
    13561 tccagggctt cttccgtcag tgctgacggg atgcggacgt gcacatcgtg gatctttcgg
    13621 cgggcatgag cccagcaggc agcttccgtt atcccaccat tgcgatacag ctcgttgaac
    13681 ccggcgtacg catccgcttg cagcacaccg ctgaagcaag caagatgagt ctgcggatgg
    13741 atgctttttc tgtccgggct gtaagcgaac cacactgcag gtgccaacgc tgacccggca
    13801 ttgcggtcat cacgaacata cgcccacaac cgcccggtct tcgtcttctt attacccggc
    13861 agcagtacct ggaccggggt atcatcggca tggagtttgc cgtcagtcat gacatagcca
    13921 tgaagcgcct cttccagcgg agacagcagc cggcagcatg catccaccca gcccgacagc
    13981 agtgaacggc tcagctccac accttgccgg ccgtatattt ctgactggca atacagcggg
    14041 gtgtgctctg catacttcga ggtcagcacg cgggccagca gccccggtcc ggcgataccc
    14101 cgctcgatgg gccgcgaagg tgcaggtgcc tgcacgatgg tatcgcactg agtacaggca
    14161 tgtttttccc gtaccgtccg gataacccgg aaggcgctac gcatcaactc cagctgttcg
    14221 gcggtatcct cgcccagata gctcagtgaa ccgccgcagt tcgggcagca cggcgccgca
    14281 ggcaacagtc gcttttcgtc acggggtagt gattcaggga acggcttacg ggtgcgggtc
    14341 tgacgcaacg gacgctgtac tgccgggtca tacaccctac cagtcagcgt atcgctctct
    14401 ttctgaagcc ggttcagatc ggcttccatt tgtgcgatac ggcgggagac tttttcggaa
    14461 cgactgccga agttcatccg gcggagttta tccagctgcg cctgcagatg gtctatttcg
    14521 cgctcccggt tgctcagctt ttcctgcagg gcgtggatca gcgcttcctg ttcggccagg
    14581 cgctgtttca gcaggaagat gtcgtcagaa gagatgtcgt tcataagccc gtattttacc
    14641 gggcttattc tgtgacaacc aggataaaga gatttacagc atggtcaggg aggtcagcag
    14701 ccgcttgggc tgtcgccagt cgataccttc cagcagcatc gccagctgcg cctgcgtaag
    14761 gaacactttg ccatcacggg ctgacggcca ggcgaagcgc ccacgctcca gccgtttggt
    14821 caggaggcac agtccgtcgc cggtggacca cagcagttta acctgactgc cgctgcggcc
    14881 ccggaaaatg aaaatatggc cggacatggg atcgtctttc agcgccgttt gtactttcgc
    14941 agccaggccg ttgaagctat ttctcatatc ggtgataccg gcaaccagcc aaattttggt
    15001 cccggaaggt aacgggatca tcgcttcagt tcctgtatca gcagagtcag gagcttttcg
    15061 ctgacattgc cattgaagcg gagcgtcccg tgccggaacg ttacctcaca gctgatactg
    15121 agggtttccg ggtcctctgc gagcgattct ggctgttcgg cagctgcatc gagagtcaca
    15181 ggaagtagct gggggctctc tgaagaaggt aatagcagct ttccctcgcg ccattgttgt
    15241 cgccatttga acaacagatt ggcgttaatg ccattttcaa gagcaagttt tgagatggat
    15301 atcccgggtt cacaggaggc agcaacgagc agctgtttaa attcgggagg ataattaggg
    15361 cagccttttc g
//
LOCUS       DAEBXN010000096        15255 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.096, whole genome shotgun sequence.
ACCESSION   DAEBXN010000096 DAEBXN010000000
VERSION     DAEBXN010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15255
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.096"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            208..1266
                     /gene="citC"
                     /locus_tag="KJL99_003135"
     CDS             208..1266
                     /gene="citC"
                     /locus_tag="KJL99_003135"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HBD4437769.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1281..1577
                     /gene="citD"
                     /locus_tag="KJL99_003136"
     CDS             1281..1577
                     /gene="citD"
                     /locus_tag="KJL99_003136"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HBD4437770.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILHARLEALLARASGIPALPWEDCQ"
     gene            1574..2482
                     /gene="citE"
                     /locus_tag="KJL99_003137"
     CDS             1574..2482
                     /gene="citE"
                     /locus_tag="KJL99_003137"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HBD4437771.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHAHRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2493..4025
                     /gene="citF"
                     /locus_tag="KJL99_003138"
     CDS             2493..4025
                     /gene="citF"
                     /locus_tag="KJL99_003138"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HBD4437772.1"
                     /translation="MTQKIEQSQRQERVAAWNRRTECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVNRVGDAAKIGAGTTRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDRQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            4029..4579
                     /gene="citX"
                     /locus_tag="KJL99_003139"
                     /pseudo
     CDS             4029..4579
                     /gene="citX"
                     /locus_tag="KJL99_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
     gene            4554..5432
                     /gene="citG"
                     /locus_tag="KJL99_003140"
     CDS             4554..5432
                     /gene="citG"
                     /locus_tag="KJL99_003140"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HBD4437773.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPTDLDYLRQFDRECIERNLSP
                     GGSADLLILTCFLAQI"
     gene            5483..6945
                     /gene="citT"
                     /locus_tag="KJL99_003141"
                     /pseudo
     CDS             5483..6945
                     /gene="citT"
                     /locus_tag="KJL99_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
     gene            7059..7865
                     /gene="rna"
                     /locus_tag="KJL99_003142"
     CDS             7059..7865
                     /gene="rna"
                     /locus_tag="KJL99_003142"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HBD4437774.1"
                     /translation="MKAFCRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKTDFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            8094..8504
                     /gene="rnk"
                     /locus_tag="KJL99_003143"
     CDS             8094..8504
                     /gene="rnk"
                     /locus_tag="KJL99_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HBD4437775.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(8735..9973)
                     /locus_tag="KJL99_003144"
     CDS             complement(8735..9973)
                     /locus_tag="KJL99_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
                     /protein_id="HBD4437776.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITVTAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRLQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNVQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            10194..10622
                     /gene="uspG"
                     /locus_tag="KJL99_003145"
     CDS             10194..10622
                     /gene="uspG"
                     /locus_tag="KJL99_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HBD4437777.1"
                     /translation="MYKAIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10743..12308)
                     /gene="ahpF"
                     /locus_tag="KJL99_003146"
     CDS             complement(10743..12308)
                     /gene="ahpF"
                     /locus_tag="KJL99_003146"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HBD4437778.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAENRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGNIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12553..13116)
                     /gene="ahpC"
                     /locus_tag="KJL99_003147"
     CDS             complement(12553..13116)
                     /gene="ahpC"
                     /locus_tag="KJL99_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HBD4437779.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13488..14234
                     /gene="dsbG"
                     /locus_tag="KJL99_003148"
     CDS             13488..14234
                     /gene="dsbG"
                     /locus_tag="KJL99_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HBD4437780.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFSDPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGCKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            14444..>15255
                     /locus_tag="KJL99_003149"
     CDS             14444..>15255
                     /locus_tag="KJL99_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBD4437781.1"
                     /translation="MANLYDLKKLDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLIAKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNYHRDFLNLKEIKLEHPLP"
BASE COUNT         3747 a         3983 c         3749 g         3776 t
ORIGIN      
        1 aaataaataa aaaaccacta actctatgtg aaataaatca aaatttcacg ccgaaatact
       61 ccttaggatg tatagcgaaa ggagaaaaag atatacctcg atcacccccc tttctcccaa
      121 gtgaaaataa aaggttatca gtttgcaaca ttgaacaaca ttcgttgcaa atcgataaca
      181 acatgcacct tcaggatact atttattatg ttcggcaatg atattttcac ccgcgtaaaa
      241 cgttcagaaa ataaaaaaat ggcggaaatc gcccaattcc tgcatgaaaa tgatttgagc
      301 gttgacacca cagtcgaagt atttattacc gtaacccgcg atgaaaagct tatcgcgtgc
      361 ggtggaattg ccggaaatat tattaaatgc gttgctatca gtgaatccgt tcgcggtgaa
      421 ggactggcgc tgacattagc cactgaactg ataaacctcg cctatgagcg gcacagcacg
      481 catctgttta tttataccaa aaccgaatac gaggcgctgt tccgccagtg cggtttttcc
      541 acgctgacct gcgtacccgg cgtgatggtg ctgatggaaa acagcgccac gcgactgaaa
      601 cgctatgccg aatcgctgaa aaaatttcgt catccaggga acaagattgg ctgcattgtg
      661 atgaacgcca atccctttac gaatggtcac cgttatctga ttcaacaagc tgcggcacag
      721 tgcgactggt tgcatctgtt tttagtcaaa gaagattctt cacgcttccc ctatgaagac
      781 cggctggatc tggtgttaaa aggcaccgcc gatattccac gcctgactgt gcatcgcggc
      841 tcagaataca tcatctcccg cgctacgttc ccttgctact tcattaaaga acagagcgtc
      901 attaaccatt gttacaccga aattgatctg aagattttcc gtcagtacct cgctcccgcg
      961 cttggtgtaa ctcaccgctt tgtcggtact gaaccctttt gtcgcgttac cgcccagtac
     1021 aaccaggata tgcgctactg gctggaaacg ccgactatct ccgcagcgcc catcgaactg
     1081 gttgaaattg agcggctgcg ttaccaggag atgccgatat ccgcttcccg ggtacgtcaa
     1141 ctgctggcga aaaacgatct cacggctatc gcgccgctgg tccctgcagt cacgctgcat
     1201 tatttgcaga acctgcttga gcactcccgc caggacgcgg cagctcgtca aaagaccccc
     1261 gcatgagaaa caggtgaaaa atgaaaataa accagcccgc cgttgcaggc acccttgagt
     1321 ctggggatgt gatgatacgc atcgccccac tcgatacgca ggatatcgac ctgcaaatca
     1381 atagcagcgt tgagaaacag tttggcgatg caattcgcac caccattctg gacgttctcg
     1441 cccgctacaa cgtgcgcggc gtacagctga atgtcgatga caaaggcgca ctggactgca
     1501 ttttacatgc acgactggaa gccctgctgg cacgcgccag cggtatcccg gctctgccat
     1561 gggaggattg ccaatgattt ccgcttcgct gcaacaacgt aaaactcgca cccgccgcag
     1621 catgttgttt gtgcctggtg ccaatgccgc gatggtcagc aactccttca tctacccggc
     1681 tgatgccctg atgtttgacc tcgaagactc cgtggcatta cgtgaaaaag acaccgcccg
     1741 ccgcatggtt tatcacgcgc tgcaacatcc gctgtatcgc gatattgaaa ccattgtgcg
     1801 tgtcaacgcg ctggattccg aatggggtgt taacgacctg gaagccgtcg ttcgcggtgg
     1861 tgcggacgtt gtgcgtctgc cgaaaaccga taccgctcag gatgttctgg atattgaaaa
     1921 agagatcctg cgtatagaaa aagcctgtgg tcgtgaaccc ggcagcaccg gcctgctggc
     1981 ggcgattgaa tctccgctgg gcattacccg cgcagtggaa atcgctcacg cttccgagcg
     2041 tttgatcggt atcgccctcg gtgcagaaga ctatgtgcgc aacctgcgta cagaacgctc
     2101 cccggaagga actgaactgc tgttcgcacg ctgttccatt ttgcaggccg cgcgctctgc
     2161 gggtattcag gcgttcgata ccgtctattc cgacgctaac aacgaagccg gattcctgca
     2221 agaagccgcc cacatcaaac agctgggctt tgacggcaaa tcgctgatca acccgcgtca
     2281 gattgatctg ctgcacaacc tctacgcacc gacccagaaa gaagtggatc acgcccaccg
     2341 cgtcgtagaa gccgctgaag ccgccgctcg cgaaggcctc ggcgtggttt ccctgaacgg
     2401 caagatggtg gacggtccgg ttatcgatcg cgcccgtctg gtgctctccc gtgcagaact
     2461 ttccggcatc cgcgaagaat aaggcaatca aaatgacgca gaaaattgaa caatctcaac
     2521 gacaagaacg ggtagcggcc tggaatcgtc gcactgaatg cgatcttgcc gctttccaga
     2581 actcaccaaa gcaaacctac caggctgaaa aagcgcgcga tcgcaaactg tgcgccaacc
     2641 tggaagaagc gattcgtcgc tcaggtttac aggacggcat gacggtttcc ttccatcacg
     2701 ctttccgtgg cggtgacctg accgtcaata tggtgatgga cgtcatcgcg aagatgggct
     2761 ttaaaaacct gaccctggcg tccagctccc tgagtgattg ccatgcgccg ctggtagaac
     2821 acattcgcca gggcgtggtt acccgcattt atacctctgg cctacgcggt ccactggcgg
     2881 aagagatctc ccgtggtctg ctggcggaac cggtgcagat ccactctcac ggcggtcgtg
     2941 tgcatctggt acagagcggc gaactgaata tcgacgtggc tttcctcggc gtcccgtcct
     3001 gtgatgaatt cggtaatgcc aacggctaca ccggtaaagc ctgctgcggc tccctcggct
     3061 atgcaatggt tgatgccgac aacgcaaaac aggtcgtgat gcttaccgaa gaactgctgc
     3121 cttatccgca taatccggca agcattgagc aagatcaggt tgatttgatc gtcaaagtta
     3181 atcgcgttgg cgatgctgca aaaatcggcg ctggtactac ccgtatgacc actaacccgc
     3241 gcgaactgct tattgcccgt agcgctgcgg atgtgattgt caactctggc tacttcaaag
     3301 aaggtttctc catgcaaacc ggcaccggcg gcgcttcgct ggcggtaacc cgtttcctgg
     3361 aagacaaaat gcgtagccgc gatattcgcg ccgacttcgc cctcggcggt attaccgcga
     3421 cgatggttga tctgcacgaa aaaggtctga tccgcaaact gctggatgtg cagagctttg
     3481 acagccatgc tgcgcaatcg ctggcccgta accccaatca catcgaaatc agcgccaacc
     3541 agtacgctaa ctggggttcg aaaggcgcat cggttgatcg tctcgacgtg gtggtactga
     3601 gcgcgctgga aattgacagg cagttcaacg ttaacgtgct gaccggttct gacggcgtac
     3661 tgcgtggcgc ttccggtggt cactgcgata ccgcgattgc ctctgcgctt tccatcatcg
     3721 tcgcgccact ggtacgcggt cgtattccga ctctggtgga taacgtgctg acctgcatca
     3781 ccccgggctc cagtgtcgat attctggtca cagaccacgg tatcgcagtt aacccggcac
     3841 gtccggaact agcagaacgt ctgcaggaag cgggcattaa agtggtttcc attgagtggc
     3901 tgcgcgaacg tgcgcgtctg ctgaccggtg aaccacagcc gattgaattc accgaccgcg
     3961 tcgttgccgt tgtgcgttac cgcgatggct cggtgatcga tgttgtgcat caggtgaagg
     4021 aataagccat gcacctgctt cctgaactcg ccagccacca tgcggtgtca attcccgagc
     4081 tgctcgtcag ccgggatgaa aggcaagcac ggcaacacgc ctggctcaag caccatcctg
     4141 ttccactggt ctcctttacc gtggttgcgc ctgggccgat taaagacagc gaggtcacac
     4201 gccgaatttt taatcatcgc gtgacagcct tgcgtgcctt agccacaaag cagggctggc
     4261 aaattcagga gcaggctgca ctggtttccg ccagcgggcc ggagggcatg ttgagcattg
     4321 ccgccccggc tcgcgacctc aagctcgcca ccattgagct tgaacatagt catcctctcg
     4381 ggcggttatg ggatatcgat gtcctgacgc ccgaaggcga aattctctcc cgccgcgact
     4441 attcactgcg cctcgccgct gcctgttgtg cgagcaaagc gcagccgtct gcgcgcgtgg
     4501 aaaaacccat caactgaccg atttactcaa ccgcatggag gcactgctga acgatgtcga
     4561 tgcctgcaac gtcaactaaa accacaaagc ttgcgacgtc attaatcgat gagtacgccc
     4621 tgctgggctg gcgcgccatg ctgactgaag tcaatctgtc accgaaacca ggcctcgtgg
     4681 atcgcattaa ctgcggtgcg cacaaagata tggcgctgga agatttccac cgcagcgcgc
     4741 tggcgattca gggctggtta ccccgtttca ttgaatttgg tgcctgtagt gcagaaatgg
     4801 caccggaagc ggtactccac ggattacgcc caattggtat ggcttgcgaa ggtgatatgt
     4861 tccgcgccac tgcgggcgta aacacgcata aaggcagcat tttttcttta gggctgctat
     4921 gtgcggcaat tggccgtttg cttcagctca accagccggt tacgccaaca accgtttgtt
     4981 ctacggcggc aagtttctgc cgtggcctga ccgatcgcga actgcgtacc aataattcac
     5041 gactgacggc aggtcaacgg ttgtaccagc agcttggcct taccggcgca cgcggtgaag
     5101 ccgaagcagg ttatccactg gtgatcaacc acgccttgcc gcattacctc actctgctgg
     5161 atcaggggtt agatcctgaa ctggcattgc tcgatacctt gctcctactg atggctacca
     5221 acggcgatac caacgttgca tcgcgcggtg gcgagggggg cctgcgctgg ctacagcgcg
     5281 aggcgcaaac attattgcaa aaagggggca ttcgaacccc caccgatctc gattatctcc
     5341 ggcagttcga cagggagtgt atcgaacgaa atctcagtcc aggcggcagt gccgacctgc
     5401 tgatccttac ctgtttttta gcacagattt aattatttaa gcacttgata aatttggaaa
     5461 tattaatttt cggagaaccc gtatgtcttt agcaaaagat aatatatgga aactattggc
     5521 cccactggtg gtgatgggtg tcatgtttct tatccctgtc cccgacggta tgccgccgca
     5581 ggcatggcat tacttcgctg tgtttgtggc aatgattgtc ggcatgatcc tcgagccaat
     5641 tccggcaaca gcgatcagtt ttattgcggt tactatttgc gttattggca gtaattacct
     5701 gctctttgat gccaaagaat tagctgaccc agcgtttaat gcgcaaaaac aggcgctgaa
     5761 atggggcctg gctggttttt ccagcaccac ggtatggctg gtatttggcg catttatttt
     5821 tgcattaggg tatgaagttt ccgggttagg tcgtcgcatt gcccttttcc tggtgaaatt
     5881 catggacaaa cgcacgctga cgttgggtta tgcgattgtc attatcgaca ttctgctggc
     5941 accgtttaca ccgtccaaca ccgcgcgtac cgggggtacg gtttttccgg tcattaaaaa
     6001 cctgccgccg ttgtttaaat cattcccgaa cgatccgtcc gcgcgtcgta ttggcggcta
     6061 tttgatgtgg atgatggtca ttagtaccag tctgagttcg tccatgtttg tcaccggtgc
     6121 ggaccaaacg tgctgggtct ggagttcgtc agcaaaattg ccggtatcca gattagctgg
     6181 ttgcagtggt tcctctgctt cctgccggtt ggggttatct tgcttatcat tgcgccgtgg
     6241 ctttcctacg tgctgtacaa accggaaatc acacacagtg aagaagtggc aacctgggcg
     6301 ggtgatgaac taaaaaccat gggtgcgctg acacgcagag agtggacgct gattggcctt
     6361 gtattgctca gcttaggttt gtgggtattt ggcagtgaag tcattaatgc tactgcggtt
     6421 ggtctgctgg cagtttcgct aatgctggct ctgcacgttg tgccgtggaa agacattacc
     6481 cgctataaca gcgcatggaa cacgctggtc aacctggcaa ctctggttgt gatggctaac
     6541 ggcctgactc gttctggttt tattgactgg ttcgccggta ccatgagtac gcacctggaa
     6601 ggattctcac caaacgcaac ggtgattgta ctggttctgg tgttctactt tgcacactac
     6661 ctgtttgcca gcctgtctgc gcacaccgca accatgctgc cggttattct ggccgtcggt
     6721 aaaggtattc cgggcgtacc aatggaacaa ctgtgtatcc tgctggtgct gtctatcggt
     6781 atcatgggct gtctgacgcc gtatgcaacc ggtcctgggg tgattattta cggctgtggc
     6841 tatgtgaaat caaaagatta ctggcgtctt ggcgcaatct tcggggtgat ttacatctct
     6901 atgttgctgt tggttggctg gccgattctc gccatgtgga actaatacgt tcgccaaacg
     6961 ctgaataaat catcacgccc gccaggtaag cccacctggc gggctttttt atgatttaat
     7021 agatagtatt cagtgtatgt atgagttcca cacccattat gaaagcattc tgccgtaacg
     7081 ccgcgttgct cgcggtttct ctgcttccct tatcttctgc caacgccgta gcgttgcagg
     7141 caaaacagta tggcgatttt gatcgctatg tcctggccct ctcctggcaa accggatttt
     7201 gccagagtca acacgatcga aatcgtaacg aacgagatga atgtcgcctg caaaccgaaa
     7261 cgaccaacaa aactgatttt ctgaccgtac atggtctgtg gccaggattg cctaaatcgg
     7321 ttgctgcccg tggtgttgat gaacgccgct ggatgcgctt cggttgcgct actcgcccaa
     7381 tcccgaatct accagaagca cgcgccagcc gaatgtgttc atcgccggaa accgggttat
     7441 cactggaaac ggccgctaaa ctaagtgaag tcatgccagg agctggcgga cgttcctgcc
     7501 tggaacgcta cgaatatgcc aaacacggtg cctgctttgg ttttgatccg gacgcatact
     7561 tcggtacgat ggtacgcctg aatcaagaaa ttaaagagag cgaagccgga aaattccttg
     7621 cggataatta cggtaaaaca gtgagccgcc gtgactttga cgccgccttt gccaaaagct
     7681 ggggaaaaga gaacgtgaaa gcagttaagc taacgtgcca gggtaaccct gcgtatttga
     7741 ctgaaattca gatctcgatc aaagctgacg ccatcaacgc tccgctttct gcaaactcat
     7801 tcttgccaca acctcaccca ggtaactgtg gcaaaacctt tgtgattgat aaagcgggtt
     7861 attaatcctc tgatttacaa aacacgctaa ttcgtcttcc ataaccatgc cgattaagaa
     7921 ttatacataa tgcgctgcat tgaggaattc cctgaatgtg acgcaaatca cttcaaagag
     7981 tgagcaacag tcagtatcat catgtgagtt aaaccctcgc cgcctgacgg tgagggtttt
     8041 cttttgggta tgtttatctg cgacactcgc agtaccgacg acatggagta aaaatgtcca
     8101 gaccaactat catcattaac gacctggatg ccgaacgcat cgatattctg ctggagcaac
     8161 ccgcctatgc tggtttgcca atcgccgacg cgttaaacgc agagttggat cgcgcccaaa
     8221 tgtgttcgcc agaagagatg ccacacgacg tggtgacaat gaacagccgg gttaaattcc
     8281 gcaatcttag cgatggcgaa gtgcgtgtgc gcacgctggt gtatccggca aaaatgaccg
     8341 atagcaatac tcagctttcc gttatggctc cggtaggtgc cgcactgctg gggctgcgcg
     8401 ttggcgattc cattcactgg gaacttccgg gcggcgttgc aacccacctt gaagtgctgg
     8461 aactcgaata ccagccagaa gctgctggcg actacctgct ttaatcccat ctgcacaagc
     8521 gataactcgc tttgcccaga ggatgcactc gcatcctctt tacgcaaaat cagcaatttt
     8581 tcctggttct cactgttcac ttttgctgca aaattattcc gtttcgtttg tgggaataac
     8641 catggcagaa gcattttaca tcctgatagg atttctgatt atggcggcga ttatcgtcat
     8701 ggctgtcctt tacctcgaaa accactcctg acgatcatat tgttcccccc ggcatcgcat
     8761 tcaccagacc tgaaaccgcc ttctgcgccg cctctgcgct ttgtgcaccg ggtaccagaa
     8821 tcaccttacg gcactcctct tcacgttttt cgaaaatctc atatccccgg gcggcctctt
     8881 caaacggcat atagtgggta acaatttctt ctggtttcag taatcctttc tcaattaacg
     8941 gtaataattc tcccagccat gcgtgaacgt gggtctgtcc cattttaaac gtcaaccctt
     9001 tatcaaaggc gtcgccaaac aggaaaccgt gaataaatcc agcgtagacg cccggtacgc
     9061 taacaatgcc gccacgcctg accgccgcaa tacactggcg caacgcttta ccgctgctgc
     9121 cctccagttt caggttagtc agcaccgttt ccgtggtgct gcctttcgct tcaaaaccga
     9181 cggcgtctat tactgcatcc acgccccggt gacctgccgt ttgttcaata attgactgtg
     9241 ccggatcgct atcttcatcg aaattaatcg ggatcgcgcc gtagcggtca gcggcgaaac
     9301 gcaagcggta gggatgatga tcaacaacaa aaatctgttc cgcaccgagc aaccgtgcac
     9361 aggcgattgt caacaatccc acaggaccag caccatagac tgcaacgctt gaaccttgtt
     9421 ggatctgcac attttttgct gcctgccatg ccgttggcag aatatcagaa aggaaaaggg
     9481 ctttatcatc tgaaagcaaa ggcggtactt taaacggccc cacattccct ttcgggacgc
     9541 ggacatattc cgcctgcccg ccaggaacgc caccatacag gtgactataa ccaaacaatg
     9601 ccgcgggagc aggtatctgt tttttattga gcgcagcgcc tttacccgca ttggtatttt
     9661 cgcaggcggc atattgctgt aatcgacaga aaaaacagtc gccacaagca atgacgaacg
     9721 gaattaccac ccggtcgcct ttttgcaaat tttttacgtc cttcccggtt tcaacgactt
     9781 cccccataaa ttcatgacca aaaatatcgc catgtttaac ctggggtatt ttgcctcgat
     9841 aaagatggag gtcagagcca cagatcgccg ttaccgtaat acgcagaata atatcatctg
     9901 cctgttcaat gcccggatcg ggaacatttt ctacctgaac gtgatgtggg ccgtgataag
     9961 tcaatgcttt catgcaacct ccagagacag ttcaaaaaag agtcagtctt taagggtagc
    10021 tatcacagcg ggaaaacggg cggtaccgca ggttttggga gaattcctag tgttcagtga
    10081 gaatatatta atcacagaaa ccgttcgtaa ggaaaaagaa ccagacaaat atcagggata
    10141 aatattataa tcatggagaa tgatttgttc atgattaaca gggagaaagg gttatgtata
    10201 aggcaatcat tatgccagtt gatgtatttg aaatggaatt gagcgacaaa gctgttcgcc
    10261 acgctgaatt cctcgcccag gatgacggag ttattcatct acttcacgta ctacccgggt
    10321 cagccagcct gagcctgcac cgttttgccg ctgatgtgcg tcgttttgaa gagcatctgc
    10381 aacatgaagc agaagaacgt ctgcaaacga tggtcagcca cttcaccatc gatccttccc
    10441 gcattaaaca acatgtccgt tttggtagcg tgcgggatga agtcaatgag ttggcagaag
    10501 aactgggggc tgatgttgta gttatcggtt ctcgcaaccc atcgatttcg acccatctgt
    10561 taggttctaa cgcctcgagc gtgatccgcc acgccaatct gcctgtactg gtcgttcgct
    10621 aacagataaa agtatttatg ccgccctgcc tggaacatcc cggcagggct ttttaatgcc
    10681 ataaaaaagc cgccaggttt gaccccggcg gctaagcaat tgcaggtgaa tcttacttct
    10741 tcttatgcag ttttggtgcg aatcaggtag tcaaaagcac tcagagaggc tttggcacct
    10801 tcgccagtgg cgatgatgat ctgcttgtac ggaaccgtcg tacagtcacc cgctgcgaac
    10861 acgcctttga cgttggtttc acatttcgca tcaatgataa tctcgcccat gcggttacgt
    10921 tcgactgcgc cttccagcca gttggtgttc ggcagcagac caatctggac aaaaataccg
    10981 gccagttcga tgttgtgaat attgccgctg acacgatctc gatattccag accaacgact
    11041 ttgctgccgt cgcctttcac ttccgtggtt tgcgcattca gaataatgtc gacgtttttc
    11101 aggctgcgca gtttgtcctg cagaacctgg tcggctttca tttctggcgc aaattccagc
    11161 agcgttacgt gctcaacgat acccgccagg tcgattgccg cttccacgcc ggagttaccg
    11221 ccgccgataa ccgctacgcg tttgccttta aacagcgggc cgtcgcagtg cgggcagtag
    11281 gtcacgcctt tagtgcgata ctgatcttcg cccggaacgt tcatgttgcg ccattttgca
    11341 cccgtcgcca cgataatgct gcgtgctttc agtaccgcgc cagaagctgt ttcaatctgg
    11401 tgtagaccac cttcaaccgc tgccgggatc agtttgctgg cgctctggct gtcgatcaca
    11461 tcaacgtcgt attcatcaac gtgaactttc agtgcgcctg ccaacttctg cccttcagtc
    11521 ttcggtacag aaatgtagtt ttcgatatca acggtatcga ggatctgacc accaaaacgt
    11581 tcgcccatca gaccggtacg gatgccttta cgtgcggagt aaattgctgc cgctgcaccc
    11641 gccgggccgg aaccaacgat taatacgtca taagcatcac gcttgttcag ctcttctgcc
    11701 gcacgatttt ccgcgccagt atcaattttg gcaacgattt cagtcaacgt catgcggccc
    11761 tgaccaaact ctttcccgtt tacgaacact gccggaacgc ccatcacgtt gcgatcggtg
    11821 atttcgttct ggaaggtgcc gccgtcaatt gcagtgtgct tgatgcgcgg gttcaatacg
    11881 ctcatcaggt tcagcgcctg caccacgtcc gggcagttat ggcaagagag cgagtaatag
    11941 gtttcgaatt caaaatcacc gtcaatatgg cgaatctgct ccagcagaga ctgcgcttct
    12001 ttcgacggat gaccaccggt ccacagcaac gccagtacca gcgaggtgaa ctcgtggccc
    12061 agcggggagc ctgcaaaacg tggtccctgg ttggaacctg ggttggtgat caggaaagac
    12121 ggcttacgca ctggcaagct gttatcttct ttaaaggtga ctttgtctga cagttctgcg
    12181 atttcagcca acagttcctt gatttctgcc gatttagcgc tgtcatccag cgtggcaatt
    12241 aactcaacag gcttggtcaa tttctcaagg taagccttga gttgagtttt catatttgtg
    12301 tcgagcatgt ttatctcctg ggctttaaac atcatcatgc aagcggcctc tgcggctacc
    12361 tggatgcagc ttgcatcatg gtgcggggaa gaaaatcggg tgccgcagca cccgaagaat
    12421 taggtgaatt tccgaagtat ttcgcgtcag agcaaggcga ctagcctgtg aatccccggg
    12481 agcttacaca agtaagtgac cggggtgagc gggcgacgcc aacgccgcta tggcgtgaaa
    12541 gacgaaggaa atttagattt taccaaccag gtccagagac ggagccagag ttgcttcacc
    12601 ttctttccat ttagccgggc aaacttcacc tgggtgagaa gctacgtact gagctgcttt
    12661 gattttacgc agcagatcag acgcgtcacg gccaatgcct tcagcggtaa cttcgattgc
    12721 ctggatgata ccctgcgggt caacaacgaa ggtcgcacgg tcagccagac cttcatcttc
    12781 acgcatgttg tcgaagttac gggtcagggc gccagtcggg tcgccgatca tcgcatattt
    12841 gattttagcg atggtttcag agctgctgtg ccatgctttg tgggtgaagt gagtatcggt
    12901 agatactgcg tatacgtcta cgcccagttt ctgcagttct tcgtagtggt cagcaacgtc
    12961 acccagttcg gtcgggcata cgaaagtaaa gtcagccggg tagaagaaga agacgctcca
    13021 gcggccttcg gtatcttttt cggtgatttc gatgaattcg ccgtttttga atgcctggtt
    13081 tttaaaaggt ttaattttgg tgttaatcaa ggacatctat acttcctccg tgttttcgat
    13141 gagatgtaag gtaaccaatt tttgccgatt cggctaatgc gtttccatta tcaaatcgat
    13201 aagttttacc ttacaacctt cgtaagacaa ctttgtgaac tttgcaccgc cagcggcgat
    13261 aaaaagtaaa atggccccct ttccaggtga ccgtgtaatc tgaactaccc tttggtatct
    13321 tcagcgcctg cctgcaggct gaggtgttgc ccttacaaat gcaacaacga catggattac
    13381 aacaccctca taaacaaagg gcaatcacct gatctaagct cttacctatg acagtgatag
    13441 gttatgcctt ttactcgact tttgcactga ctgaaaagaa caaattaatg ttaaaaaaga
    13501 tacttttact ggctctgctt cctgcaatcg ccttcgcaga ggaacttcct tccccagtaa
    13561 aagcgattga aaaacagggc attacaatca tcaaaacgtt cgatgccccc ggaggaatga
    13621 aaggttatct cggaaagtat caggatatgg gcgtcaccat ctacctgact cccgatggta
    13681 agcacgctat ctctggttac atgtacaacg agaaaggtga aaacctgagt aacacactta
    13741 tcgaaaaaga aatttacgca ccagccggac gcgaaatgtg gcaacggatg gaacaatccc
    13801 actggctcct cgacggtaaa aaagatgcgc cggtcattgt ctacgtcttc tccgatccgt
    13861 tctgcccata ttgtaaacag ttctggcagc aggcgcgccc gtgggtagat tctggcaaag
    13921 tgcaattaag aacattgttg gttggggtta tcaagccaga aagcccggcg acagcagcgg
    13981 caattcttgc ctccaaagat cccgcaaaaa cctggcaaca atatgaagcc tctggttgca
    14041 agcttaagct aaacgtgcct gcaaacgtaa gtacagagca aatgaaagtg ttaagtgaca
    14101 atgagaaact gatggacgat ctgggggcaa atgtcacgcc ggctatctat tacatgagta
    14161 aggaaaatac gctgcaacag gccgtggggt tgcccgatca gaaaacgctt aatatcatta
    14221 tgggaaataa ataaaaggta attcatggat ggttgaacta tatccctgct tttcaaccat
    14281 cctaaaaaag catgaatttc tcgctctacc ctaagtggct gtgctatttt agaaaaactt
    14341 aatcctgagt taagcaaaaa aacataatca ataaaaatac atacgcaaaa aacattgatt
    14401 aagtgaatat accatggaag aaaaatataa accggagtag tgtatggcca atctctacga
    14461 cttgaaaaag ctcgacttaa atcttcttgt catatttgag tgtatttacc agcatttaag
    14521 cattagcaaa gctgctgaat cactatatat caccccatca gccgtgagcc agtcgttaca
    14581 acgcttacgt gcgcaattta atgacccttt attcattcgc tcaggaaaag gtatcgcgcc
    14641 gacgacaaca gggttgaatc tgcatcacca tcttgagaaa aacctcaaag gccttgaaca
    14701 aacaattaat atagtcaata agtccgaact aaagaaaaac tttattatct acggccctca
    14761 gctcatttct tgctctaata atagtatgct gatccgctgc ctacggcaag atgcctctgt
    14821 cgagattgag tgccatgata tcctcatgtc agcagagaat gctgaagaac tgttggttca
    14881 ccgcaaagcc gatctggtga tcacccaaat gccagtgatc agccgttccg tcatttgcat
    14941 gccattgcat acaattcgca atactctaat ttgtagtaat aagcatccgc gaattactga
    15001 caatagcact tatgaacaaa ttatggcaga agagttcacc caattaatag ccaaatcagc
    15061 aggagttgat gatattcaaa tggaaataga cgagaagttt atgaatcgca aaatttcatt
    15121 tcgtggttcc tctttgctga caataattaa tagcattgca gttacagatt tactgggaat
    15181 agtcccctat gaactttata actaccaccg tgactttctt aatttaaaag agataaaact
    15241 agaacatccg ttacc
//
LOCUS       DAEBXN010000097        14978 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.097, whole genome shotgun sequence.
ACCESSION   DAEBXN010000097 DAEBXN010000000
VERSION     DAEBXN010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14978
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.097"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(519..1460)
                     /gene="fabY"
                     /locus_tag="KJL99_003150"
                     /gene_synonym="yiiD"
     CDS             complement(519..1460)
                     /gene="fabY"
                     /locus_tag="KJL99_003150"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HBD4437782.1"
                     /translation="MYHLQVPQTEEELERYYHFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMVVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(1505..1942)
                     /gene="dtd"
                     /locus_tag="KJL99_003151"
     CDS             complement(1505..1942)
                     /gene="dtd"
                     /locus_tag="KJL99_003151"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HBD4437783.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(1939..2811)
                     /locus_tag="KJL99_003152"
     CDS             complement(1939..2811)
                     /locus_tag="KJL99_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HBD4437784.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(2805..3404)
                     /gene="yihX"
                     /locus_tag="KJL99_003153"
     CDS             complement(2805..3404)
                     /gene="yihX"
                     /locus_tag="KJL99_003153"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HBD4437785.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(3503..4288)
                     /locus_tag="KJL99_003154"
     CDS             complement(3503..4288)
                     /locus_tag="KJL99_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBD4437786.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEKKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(4322..5218)
                     /gene="yihV"
                     /locus_tag="KJL99_003155"
     CDS             complement(4322..5218)
                     /gene="yihV"
                     /locus_tag="KJL99_003155"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HBD4437787.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDAGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            5386..6282
                     /gene="yihU"
                     /locus_tag="KJL99_003156"
     CDS             5386..6282
                     /gene="yihU"
                     /locus_tag="KJL99_003156"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HBD4437788.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            6306..7184
                     /gene="yihT"
                     /locus_tag="KJL99_003157"
                     /pseudo
     CDS             6306..7184
                     /gene="yihT"
                     /locus_tag="KJL99_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709681.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
     gene            7201..8442
                     /gene="yihS"
                     /locus_tag="KJL99_003158"
     CDS             7201..8442
                     /gene="yihS"
                     /locus_tag="KJL99_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HBD4437789.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYALVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLGSWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVITKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            8580..9482
                     /locus_tag="KJL99_003159"
     CDS             8580..9482
                     /locus_tag="KJL99_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HBD4437790.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTTTSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEMTITHPQQALSVSLCSDQPWVQVYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQRK"
     gene            9681..11717
                     /gene="yihQ"
                     /locus_tag="KJL99_003160"
     CDS             9681..11717
                     /gene="yihQ"
                     /locus_tag="KJL99_003160"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HBD4437791.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHNKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRITSFGKRVMWNWKWNSENYPQLDSRIKQWYQEGVQFLAYI
                     NPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYSLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLNSI"
     gene            11763..13137
                     /locus_tag="KJL99_003161"
                     /pseudo
     CDS             11763..13137
                     /locus_tag="KJL99_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            13180..14583
                     /locus_tag="KJL99_003162"
     CDS             13180..14583
                     /locus_tag="KJL99_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD4437792.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKLYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATTLFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEKKTASVTL"
     gene            14651..>14978
                     /gene="ompL"
                     /locus_tag="KJL99_003163"
     CDS             14651..>14978
                     /gene="ompL"
                     /locus_tag="KJL99_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="porin involved in the transport of small molecular
                     weight solutes (up to 600 Daltons) across the cell wall;
                     specific substrate still unknown; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HBD4437793.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSISS
                     GGAVYLD"
BASE COUNT         3674 a         3856 c         3736 g         3712 t
ORIGIN      
        1 gatttaatag cgtttgcttc ggtgatttat tactcattag ttgtttttct aaattaacaa
       61 cagaataact ttcttgttta atgagattaa tattggcaac cattatagcc tccttcccac
      121 ggtttaagag caaacataca acataatgat tcataacata cagagcagta aatgaatgat
      181 actgacaatg tatgccagcc cattatcttt gcataatatt tgtgagctat agattttaaa
      241 aatatttatg aaaaaagtat ggcaccagag ggggttttga ctatcaaaaa tcgctcaaac
      301 ctgaactaat atgatgagtt aagcattatc tgcaataatt gccactcagc tctacaaata
      361 atatttaaat gctcctgaac agtgatgtag caagacacgt atggtgccaa cgaagaataa
      421 aaatttataa agttcttatt caatataacc ggaatagtca gggactgatg taaatgaccg
      481 gaatgaatga gagaggcagc accgcccctc tccataagct actcttcttc gttcccgccc
      541 tcttcatacg ggccaaatgg cttcgcgggc agaacgatat acgtgccttc aaacactgca
      601 cccggcgtct cgtcgccaaa gatttcgacc tgcatctgca cccgtgcttt tcgtccgcgc
      661 gccagacggt cgagatcgcc gcttaaggca ccaaggtcgg ctaccgcatg gggtttaccg
      721 ctaatcggtt tgctgtagcg gatatgcgca tccgccagaa taatcgttcc gccgaggtgg
      781 cgttcacgca gcatcagcca gataagcccc caaccggtga gcgtcgccag cgagaataaa
      841 ctcccggcaa acagcgtatg gtgcggattc tgattgccgg tttccggcat ggtggtgata
      901 aatttttgcc cggtatattg ttgaatacgc acgcccattt tttcactaag cgggatatgt
      961 tcgtaccacg cctgttgcag ctgcgcgcac cagtcgccgc gatgcaaaat atcatccaga
     1021 gtggcgacgg gcttaatcat caaaaaatgg cgaatcggcg tggtggttgg cgtggtgatt
     1081 tctccctgat taacaaaccc cagcttggcg aaaaactcca ccgcgtcttc acgggcgcta
     1141 caggtcacgc gcttaacgcc ttcctgacgc gccaccgact ccagggtcat cgccatcagc
     1201 gttcctaacc ctttgtcctg cacgtccggg tgaacgacca taaagcgaat ggacgcttca
     1261 ttgtcggcat taatatacaa tcggcctacc gccaccagat taccctgctc gtcgacgacc
     1321 atctggtgat gcgccatcgc atcccacgcg tcgcgttccg aaccttttgg ttgatgcagg
     1381 ggcttacgca acatttccca gcgaaagtga tagtaacgct ctaactcttc ttctgtttgt
     1441 ggaacctgaa ggtgatacat agcgatactc tctcttgttt cccgtgacaa ccctggaagc
     1501 tggctcatac ctgcaaccag aatgtcacgg ggccatcatt gaccagcgat acctgcatat
     1561 cggcagcgaa gcgtcctgtt tgcgtgttca tctcttgctg gcggcagcgt tcgacgaaat
     1621 agtcatataa cgcctctgcg cgatccggtg atgcgccttt ggagaaactt gggcgcatcc
     1681 cccgttcggt atctgcggcg agggtaaact gggaaaccac cagtacactg ccgcccgcct
     1741 gttgcacgtt gagattcatc ttgccttcgg catcgctaaa gatgcggtag ccgagcacac
     1801 gctcgcacag acggtttgct ttctgttcgt cgtcatcctt ttcgacaccc aataacacca
     1861 aaagtcccgc gccaatttcg cccgtcactt ctccctccac ggtgacgctg gcacgggtta
     1921 cgcgttgaat taatgcaatc atggttcgtc gtcttcttct tgttcagctg cttgttttag
     1981 tttgcggtat tccccgagag tgacagtaat ttccgcgcca agcaagacga tacaccatgt
     2041 ccagtagacc caaacaaaga gaatggggat caccgccagc acaccgtaaa tgagctgata
     2101 tgacgggaac atggtgatat aaagcgcgaa acctttcttt cctgcttcga acaggagtgc
     2161 ggcgacaaac gcgccgacaa tcgcgtcgcg gttaggtacg cggatggtag gaacaatgct
     2221 gtacagcaac cagaaggaga tccacgacaa cagcaacgga aaaatacgca gcacgttgtc
     2281 gatgacagta ttgagatcgc tcgcccagcg cagggagagc aaataggaac tgatcgccag
     2341 actggcccct gccagcagcg gccccagcgt taaaatcatc cagtacacgg cgaacgagta
     2401 aatttttggt cgcgctcgtt tactgcgcca gatggtattc aacgcgctat cgatggagta
     2461 catcaacaat aacgcggtga cgatcagccc gcacgcccca acggcggtca tcttgttgga
     2521 attagcaaca aattgttcga tataccgctg aataacatcg ccagtagcag gcagaaagtt
     2581 ggcaaaaata aagtgacgta actgaatgct gacgtcggaa aacatgggaa aagcggcgaa
     2641 aagcgcaaaa acaacggcaa ccagcggcac taatgagagc aacgacacat aggcaaggtt
     2701 acctgccagg gttgtcatgt tgtcctcatc aatacgttgc cagagtagtt ttagccaggc
     2761 ccatagtgga cgggtacggt gcctggcttt gtcctgaatg gtttttagca taacaccttc
     2821 gcgaaatagt ccgggatggt ggttttatct ttcaccagaa tactggtaat gcctagctga
     2881 ttagctcctt ctatattatc ggcgttatcg tcgaaaaaga ccgtatcgct gggtgaaaaa
     2941 ccttccgcct gcaaaacatg ctggtaaatt cgtgcttcag gtttgcgcat ccccagatct
     3001 tgcgacagat agatatggtc agcagcatca cgaatttccg ggtattcttc cggccagaag
     3061 gtggtatgca ggcggttggt attggaaagc accaccacgc gatgcccctg ctcacgcagt
     3121 ttatgcatga tggcgatcac ttccgggcgc agcgcaacaa acaccgcctg ccagccgtga
     3181 gagaactgct cgtagcttag cggtagagcc atctcatgac acagcgcctc tgcgaacgct
     3241 tcgtcgctaa tttccccacg ctcatgctga tgaaacgcct cccccatatg aaaactcttc
     3301 ttaagcgatg ccagcggaat acgcgttaaa tcgctccagg ctcccagcac acggttaaag
     3361 tcgatatcga caatcacatt acctaaatca aagatataga gcatttttgc ctcctttcgc
     3421 gccacaagaa attaactgta gcgggaaagg atgggtttgg ctatgtggca aggtatgaag
     3481 ccagattaac ggtcacaaaa atctacgcgt cttcctgggg aagaatgatt tcaatttggc
     3541 tgtcttgtaa gcgtgatttt agcgcagcgg gcggcagctc gtcggtaaag agcgcagtga
     3601 cctgtgccac gttaccaatt tcaaccgccg ccgaagcatg atacttagtg tgatcggcga
     3661 ccagcagaat atttctcgcg tgcgccatca tcgttttcac cacgttagct tcgtttacat
     3721 caaactccat caacgcgcca tcgctctcaa tcgccccaac gcttgttacc agataatcag
     3781 cacgaaaatc agccacaaag gacgccgctg aagggccaat gatcccgcta ttatgagagc
     3841 gcaacgtacc gccgggcacc atcacttcaa agcgcgggtt gtggtaaaga atatgcgcca
     3901 cacgcaggct gttggtgatt atccgcaaat gattatggtt aagtaacgcc cgggcaacat
     3961 gctcaacagt cgtaccaatg gtgataaata ttgttgaacc atcaggaata tagtctgcca
     4021 ccgcttcggc aatcgctttt tttttctcgg tttgcgaaac ctcacgctgc tcgaacgccg
     4081 tattaacgac gctggaagcc cgacccgcgc caccgtgatg gcgcgtaatc aggccttgct
     4141 cgcttaattt acgaatatcc cggcggaccg tctgcgtgga gacatccagc agatttgcca
     4201 gctcatcaat attcatatac ccacgctcgg cgatcagcat gaggagttgg tcgtgccgcg
     4261 ggttaccggt tagttcggta aggctcatga aaattcctcg aaaaaccatc accctggcat
     4321 tttatacaaa aagtgacaaa aaagatcggg tttgatcaca gtcagggatc ccggctcgtc
     4381 caccgggacg tgtgcatttt aacgccgcta caccgctggc gaagcggact gactccgcta
     4441 aatccccact tgttgccagc gccaccgcca aagcgccgtg aaaaacatct cccgcacctg
     4501 tggtatctac cacatcaact ttgaaggccg gttgatgctg acgcccacca ttttccagcc
     4561 agtcgcagcc tgcgctaccc tgggtcacat agacatgacc atttgtgagc gtttgtgcct
     4621 gttttagcgc actggccatc tctttcacgc ccgttaagcg cgccagaccc ggttctgaaa
     4681 aggccgcgtg atcgcttaat gccaccagct cactgatatc ctgcggcgta atgtccccgt
     4741 ccagaacggt catcacaccc gcctgacggg ccagggtgaa ggctttttta gcgccgtcgt
     4801 gccagcgtac atctgccagc acaacatccc actgagagaa atcaatttcc tccaaccact
     4861 ctgcgtcagg cagcagatcc gggctggggt agttaatgat tatccgctcg cctttggtat
     4921 ccaccatgat ggcggattgc gaagatttcg cctggttata ccgtttggtg taacgggtgt
     4981 taacgcccca ggattccagc tctgccagca ggctgttgcc cgcgtcgtca tcacctacgc
     5041 gaccaataaa atcgacctgc gcccccagcc ttgccgccgc aaccgctgcc gtcgccgctg
     5101 gcccgccacc aacttccgta taatttctcg ccacgtattt accgctctcc gtcggtaacc
     5161 cttccacgta atagatgcga tccatcacgg ttatacctac acaagcaaca cgaatcatgg
     5221 tcattcctta aacattttgg gatgcgttca ttttaatttc accaattgtt taaaaagtga
     5281 cggctgtcaa ttttttgacg ataaattaca aattcgatca aaaacagaca agcaaagcat
     5341 gtcataaatg acaaaaagtg acatcactgt attcaggaga gggttatggc agcaatcgcg
     5401 tttatcggtt taggacaaat gggttcgcca atggcgagca atttattgca gcaagggcat
     5461 caacttcgcg tctttgatgt gaacgccgaa gctgtgcggc atctggtaga caaaggtgcg
     5521 actcccgccg ccaacccggc gcaggccgct aaagatgccg aatttatcat taccatgttg
     5581 ccgaatggcg atctggtgcg caacgtgttg ttcggtgaaa acggcgtttg cgaaggctta
     5641 tctaccgatg cgctggtcat tgatatgtcc accatccatc cgctgcaaac cgataaattg
     5701 attgccgata tgcaagccaa aggcttcagc atgatggatg ttccggtagg ccgtacttct
     5761 gcaaatgcca ttaccggtac tctgttactg ctggctggcg gcaccgctga acaagttgaa
     5821 cgtgccacac cgatcctgat ggcgatgggc agtgagttga tcaacgctgg cggtccgggc
     5881 atggggatcc gcgttaagct catcaacaac tacatgagca tcgcgctcaa tgcgctttcg
     5941 gcagaagccg ccgttttgtg cgaagccctg aatcttccct tcgatgttgc cgtcaaagtg
     6001 atgagcggta ccgccgccgg taaaggtcac ttcaccactt cctggccaaa caaagtcctc
     6061 agcggagatc tttctcccgc cttcatgatc gatcttgccc ataaggatct tggcatcgcc
     6121 cttgatgtcg ccaaccagct gcatgtgcca atgccgctgg gggccgcctc acgggaggtt
     6181 tatagccagg cgcgcgcagc gggtcgcggt cgccaggact ggtccgccat tctggaacag
     6241 gtccgtgtca gtgccgggat gactgccaaa gtaaaaatgt aacgactgga taaaggaata
     6301 aatgaatgaa taagtacacc atcaacgaca ttacgcgcgc atcgggcggt tttgccatgc
     6361 tggcggtcga tcagcgcgaa gccatgcgca tgatgtttgc cgcggctggg gcacccgctc
     6421 cggtagccga tagcgtttta actgatttca aagttaacgc tgcaaaggcc ctctcgcctt
     6481 atgcctcggc gattctggta gatcaacaat tctgctaccg ccaggtggtt gagcaaaacg
     6541 cgattgccaa aagttgcgcc atgattgtcg ccgccgatga gttcattcct ggcaacggta
     6601 ttccggtcga tagcgtggtt attgaccgca aaatcaatcc gctacagatc aaacaagacg
     6661 gtggcaaagc cttaaaactg ctggtgctgt agcgtagtga tgaagatgcg cagcaacgtc
     6721 tggatatggt gaaagagttc aacgaactgt gccactcaca cggtctggta agcatcattg
     6781 agccagtcgt ccgcccaccg cgtcgtggcg ataaattcga tcgcgaacaa gcgatcatcg
     6841 atgccgccaa agagctgggc gacagtggcg ctgacctcta caaagttgaa atgccgcttt
     6901 atggcaaagg tccgcaacaa gagcttctct gtgcttcaca acgtctgaat gaccatatca
     6961 atatgccatg ggtgatcctc tcttccagtg tcgacgaaaa actgttcccg cgtgccgtac
     7021 gcgtggcaat gacggcgggc gcatcgggat tcctggcagg ccgtgcagtc tgggcatcgg
     7081 tcgtcggttt accagacaac gagctgatgc tccgtgacgt ttgcgcaccg aaattacaac
     7141 aacttggcga tatcgtcgac gaaatgatgg ctaaacgccg ctaagatgag gataaaagga
     7201 atgaaatggt ttaacaccct aagccacaac cgttggctgg aacaggaaac cgaccgcatc
     7261 tttgattttg gtaaaaattc cgtagtgccg actggttttg gctggttagg caataaaggg
     7321 caaatcaaag aagagatggg cacccatctg tggatcacag ctcgtatgtt gcacgtttat
     7381 tccgttgctg cggcgatggg tcgacctggc gcttacgcgt tggttgatca cggtattaaa
     7441 gccatgaacg gcgcactgcg tgataaaaaa tatggcggct ggtatgcctg cgtgaatgac
     7501 gagggcgtgg tggatgcctc caaacagggc tatcaacact tctttgctct gctgggtgcc
     7561 gccagcgccg tcacaacggg tcacccggaa gcgcgcaagc tgctcgatta caccattgag
     7621 attatcgaga aatatttctg gagcgaagaa gagcagatgt gcctggggtc ctgggacgaa
     7681 gccttcagca aaaccgaaga gtaccgcggc ggcaatgcca atatgcacgc ggtggaagct
     7741 ttcttgattg tttatgacgt cactcacgac aaaaaatggc tggatcgcgc gattcgcgtg
     7801 gcttccgtga ttatccacga cgtcgccaga aataatcatt atcgcgttaa cgaacacttc
     7861 gatacccagt ggaatccgct gccggattac aacaaagata acccggcgca tcgcttccgc
     7921 gcgttcggag gtacaccagg ccactggatc gaatggggcc gtttaatgct gcacatccat
     7981 gctgccctgg aagcccgttg cgaacaacca ccagcatggc tgctagaaga tgccaaaggt
     8041 ctgtttaacg ccaccgtgcg cgatgcctgg gcacccgatg gagcggacgg gattgtttat
     8101 accgttgact gggaaggaaa accggtggtc cgcgaacgtg tacgttggcc tatcgtcgaa
     8161 gcaatgggta cggcctacgc gctctacacc gtcactggcg atcgccagta tgaaacctgg
     8221 tatcaaacat ggtgggagta ctgcattaag tacctgatgg actatgaaaa tggttcctgg
     8281 tggcaggagc tggatgcgga caataaggtc atcaccaaag tctgggacgg caaacaggat
     8341 atttatcacc tgctgcattg cctggtgatc ccgcgtatcc cgttagcgcc cggcatggct
     8401 ccagcggttg cagcgggtct gctggatatt aatgcgaaat aactgatctg aaaatagggt
     8461 gcgggttttc ccctcaccct aaccctctcc ccaaaggggc gaggggaccg tccggtgcag
     8521 tttttgggtt tgacatcagc ctcacgggag gctgtatgtc gttaattaag gtgccttgta
     8581 tgcaaatcac caatatgcat tgcagtggac agaccgtttc acttgccgcg ggggattatc
     8641 acgcgaccat tgtgaccgtc ggtgctggtc tggctgaatt aacctttcag ggttgtcatt
     8701 tagtcatccc gcacaaaccc gaagagatgc cgctggcgca tctgggtaaa gtgcttattc
     8761 cgtggccgaa ccggatcgcc aacggctgtt accgttatca ggggcaggaa tatcagttac
     8821 ccattaatga acatagttca aaagccgcta ttcacgggct gcttgcctgg cgagactggc
     8881 aaattagtga attaaccacc accagcgtta ccctgacagc ttttctgccg cccagttacg
     8941 gctatccgtt tatgctggct tcacaagttg tttattcgct caacgcacac acgggtcttt
     9001 ccgttgagat cgcaagccag aatatcggca ctgtcgcagc gccttatggc gtgggtattc
     9061 atccttattt aacctgcaac ttaacatctg ttgatgaata tctgttccag cttccggcaa
     9121 accaggttta tgccgtcgat gaacacgtaa atccgacaac gcttcaccac gttgatgagc
     9181 tggatttaaa tttcacgcag gcgaaaaaaa tagccgcgac aaaaatcgac cacaccttta
     9241 aaaccgctaa tgatttgtgg gaaatgacca ttacccatcc acagcaggcg ctttcggtaa
     9301 gcctttgttc cgatcaaccc tgggtgcagg tttatagcgg tgaaaaatta cagcgtcagg
     9361 gactggcagt tgaacctatg agttgcccgc ctaacgcctt taattcaggt atcgacttat
     9421 tattactgga accaggaaaa ccgcaccgcc tgttttttaa tatttacgga cagagaaaat
     9481 aacttgtatt gattagcagt caaaatcatt gaagaacatt tcactcgcta aaggcaacat
     9541 tatgtatctt caatttatta agttaaaaac agctcagctt ctgtaaagtt tttatatatc
     9601 agttttatga gaatcatttt acataagcct gtaataacag gaaggcaaca atctcgttct
     9661 caactctatg gagattagtt atggatacgc cacgtccaca gttattagat tttcaatttc
     9721 atcagaataa cgacagtttt accctacatt ttcaacaacg tcttatttta acccataaca
     9781 aagataatcc ttgtttatgg attggctcag gtatagcgga tatcgatatg ttccgcggta
     9841 atttcagcat taaagataaa ctacaggaga aaattgcgct taccgacgcc atcgtcagcc
     9901 agtcaccgga tggttggtta attcatttca gccgtggttc tgacattagc gccacgctga
     9961 atatctctgc cgacgatcag ggccgtttat tgctggaact acaaaacgac aaccttaacc
    10021 acaaccgtat ctggctgcgc cttgccgctc aaccagagga ccatatctac ggctgcggcg
    10081 aacagttttc ctacttcgat ctgcgcggca aaccgttccc gctatggacc agtgaacaag
    10141 gcgttggtcg caacaaacaa acctatgtca cctggcaggc cgactgcaaa gaaaatgcgg
    10201 gcggcgacta ttactggact ttcttcccac agcctacgtt tgtcagcacg cagaagtatt
    10261 actgccatgt tgataacagt tgctacatga acttcgactt tagcgccccg gaataccatg
    10321 aactggcgct gtgggaagac aaagctacgc tacgttttga atgtgctgac acatacatct
    10381 ccctgctgga aaaattaact gccctgctgg gacgccagcc agaactgccc gactggattt
    10441 atgacggggt aacgctcggc attcagggcg ggacggaagt gtgccagaag aaactggaca
    10501 ccatgcgtaa cgcgggcgtg aaggttaacg gcatctgggc gcaggactgg tctgggatcc
    10561 gtataacctc ttttggcaag cgcgtgatgt ggaactggaa gtggaacagc gaaaactacc
    10621 cgcaactgga ttcacgcatt aagcagtggt atcaggaggg cgtgcagttc ctggcctata
    10681 tcaacccgta tgttgccagc gataaagatc tctgcgaaga agcggcacaa cacggctatc
    10741 tagcaaaaga tgcctctggc ggtgactatc tggtggagtt tggcgagttt tacggcggcg
    10801 ttgtcgatct cactaatcca gaagcctacg cctggttcaa ggaagtgatc aaaaagaaca
    10861 tgattgaact cggctgcggc ggctggatgg ctgacttcgg cgagtatctg cccaccgaca
    10921 cgtacctgca taacggcgtc agtgccgaaa ttatgcataa cgcctggcct gcgctgtggg
    10981 cgaagtgtaa ctacgaagcc cttgaagaaa cgggcaagct cggcgagatc ctcttcttta
    11041 tgcgcgccgg ttctaccggt agccagaaat actccaccat gatgtgggcg ggcgaccaga
    11101 acgtggactg gagtctcgac gatggcctgg cgtccgtagt cccggcggcg ctgtcgctgg
    11161 caatgaccgg acatggtctg catcacagcg acattggcgg ttacaccacc ctgtttgaga
    11221 tgaagcgcag caaagagctg ctgctgcgct ggtgcgattt cagcgccttc acgccgatga
    11281 tgcgcaccca cgaaggtaac cgtcctggcg acaactggca gtttgacggc gatgcggaaa
    11341 ctatcgccca ttttgcccgt atgactaccg ttttcaccac cctgaaacct tatctgaaag
    11401 aggccgtcgc gctgaatgcg aagtccggtc tgccggtcat gcgcccgctg ttcctgcatt
    11461 acgaagacga tgcacatacc tattccctga aatatcagta cctgttaggc cgcgacattc
    11521 tggtcgctcc ggtacacgaa gaaggtcgca gcgactggac gctctatctg ccggaggata
    11581 actgggtaca tgcctggacg ggtgaagcgt tccggggcgg ggaagttacc gttaatgcgc
    11641 ccatcggcaa gccgccggtc ttttatcgcg ccgatagcga atgggcggca ctgttcgcgt
    11701 cgttaaacag catctaatca catccgcccg tggggaaact catgggcaaa gggagaacat
    11761 cgatgagtca catcacaacg gaagatccag caactttacg cctgcccttt aaagagaaac
    11821 tctcttacgg tattggcgac ctggcctcta acatcctgct ggatatcggt acgctttatc
    11881 ttttgaagtt ttataccgac gttctggggc tgccaggcac ctatggcggc attatctttt
    11941 tgatttcaaa attctttact gcgtttaccg atatgggtac cggcattatg ttggattccc
    12001 gacgcaagat cggtccaaaa ggtaagttcc gtccttttat tctgtatgcg tcattcccgg
    12061 tcaccttact ggcgatcgcc aactttgtcg gcacaccgtt tgatgtcacc ggtaaaacgg
    12121 tgatggccac tattctgttt atgctttacg gactgttttt cagcatgatg aactgctcct
    12181 acggcgcaat ggtcccggca atcaccaaaa accccaacga acgcgcctca ctggcggcat
    12241 ggcgtcaggg cggcgctacg ctgggcctgc tgctgtgcac ggtgggattc gtgccagtta
    12301 tgaatcttat cgaaggtaat cagaaacttg gctatatctt cgccgccacg ctgttttcac
    12361 tgttcggcct gctgtttatg tggatctgct actcaggcgt gaaagagcgt tatgtcgaaa
    12421 cccaaccaac caatccggcg caaaagcctg gcctgttgca atctttccgc gcaattgccg
    12481 gtaaccgccc actgttcatt ctgtgtattg ccaacctctg caccttaggg gcgtttaacg
    12541 tcaagctcgc cattcaggtc tattacaccc agtacgtact taacgatccc atcctgttgt
    12601 cgtatatggg atttttcagc atgggctgta ttttcatcgg cgtgttcctg atgcctggcg
    12661 cagtcagacg ttttggtaag aagaaggtct atatcggcgg cctgctgatt tgggtgctgg
    12721 gcgatctgct caactatttc ttcggcggcg gctcggtcag ctttgtggcg ttctcctgcc
    12781 tggcgttctt cggctcagcg cctgaactgg gcgctggttt ccgacaccgt cgagtacggc
    12841 gagtggcgca ccggcgtgcg ttcggaaggc acggtctaca ccggcttcac cttctttcgc
    12901 aaagtgtctc aggcgctggc tggtttcttc cccggctgga tgctgacgca aattggctat
    12961 gtgccgaacg tcgcacaggc tgaccacacc attgaagggt tacgccagtt gatcttcatc
    13021 tacccaagcg cactggcggt agtcaccatt gtggcgatgg gttgcttcta cagcctgaac
    13081 gagaagatgt atgtccgcat tgtggaagag atagaagccc gtaaacgcac ggcgtaataa
    13141 taataaacga cgccctgcgg ggcgttataa ggagtgatta tgtctgacca taatccactg
    13201 acattaaaac tgaatctgcg ggaaaaaatc gcctatggta tgggcgacgt cggttcgaat
    13261 ttaatgctct gcatcggtac tctgtatctc ctcaaacttt ataccgatga attagggatg
    13321 cctgcttact atggcgggat tatctttctg gtcgcgaagt tttttaccgc gtttaccgat
    13381 atgctcaccg gatttttact cgactcgcgg aaaaatattg ggcctaaagg caaatttcgt
    13441 ccctttattt tatatgcagc agttccggcg gcgttaattg caacgctcca gtttatcgcc
    13501 accacctttt gtttgccggt taaaacgacg attgccacca cgctgtttat gatgtttgga
    13561 ctttcataca gtctgatgaa ctgctcgtat ggtgcgatga tcccggcaat taccaaaaac
    13621 ccgaatgagc gcgcgcagct cgcggcttac cgccagggtg gtgcaaccat agggctattg
    13681 atttgtaccg tggcgtttat tccgttgcag tcgctttttt ctgactcaac cgtcggttat
    13741 gcctgtgcgg cacttatgtt ctccattggc ggctttattt ttatgatgct gtgctacaga
    13801 ggcgtcaaag agcattatgt ggacacagcg ccaaccggac ataaagccag tattctcaaa
    13861 tctttttgcg cgatatttcg taatccacca ttgctggttt tatgcattgc taacctgtgt
    13921 actctggcgg catttaatat caaactggcg attcaggtct attacaccca gtatgtgctg
    13981 aatgatatta atttattgtc gtggatggga ttcttcagta tgggatgcat cctcgtcggc
    14041 gtattactgg tgccagtaac tgtaaaatgt tttggtaaaa aacaggttta tttagctggc
    14101 atggtgctat gggcggtggg tgatatactg aattatttct ggggaagtaa ctctttcact
    14161 ttcgtcatgt tctcttgtgt cgcctttttt ggcacggcct ttgtaaacag cctgaactgg
    14221 gcactggtac cagataccgt agattacggt gaatggaaaa ccggtattcg tgccgaaggt
    14281 tctgtttata ccggttatac cttctttcgt aaaatttctg ccgcacttgc tggcttcttg
    14341 ccaggcatta tgctgacgca aattggttat gttcccaaca tcgcgcaaag cgatgcgaca
    14401 ttacagggtt tgcgtcagct catttttatc tggccttgtg cactggcaat tattgctgca
    14461 ttgacgatgg gattctttta cacactcaat gaaaaacggt ttgcattgat tattgaggaa
    14521 atcaaccaac gcaaaaataa agagatggcg acagaaaaaa aaacggcttc cgtaacgtta
    14581 taaatgtcaa tagccgctat ttccatcctg gtgggtggcg gcctccctac gtttaaaaaa
    14641 tggacttatt atgaaaaaga ttaatgcaat aattttatta tcttctctga cctctgcctc
    14701 ggtatttgct ggcgcatacg tcgaaaacag ggaggcatat aatcttgcct ccgatcaggg
    14761 cgaggttatg ctacgtgtgg gttataactt cgatatgggc gcgggtatta tgttaaccaa
    14821 tacctacacc tttcagcgag aagatgaact aaaacatggc tataatgaaa ttgaaggttg
    14881 gtatccttta ttcaaaccaa ccgataaatt aaccatccag cccggtggct taattaatga
    14941 taagagtatc agttcgggtg gtgcagtgta tctggacg
//
LOCUS       DAEBXN010000098        14729 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.098, whole genome shotgun sequence.
ACCESSION   DAEBXN010000098 DAEBXN010000000
VERSION     DAEBXN010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14729
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.098"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..66
                     /locus_tag="KJL99_003164"
     CDS             <1..66
                     /locus_tag="KJL99_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HBD4437794.1"
                     /translation="LINVNFFSSDRTSFCECNRFP"
     gene            322..862
                     /locus_tag="KJL99_003165"
                     /pseudo
     CDS             322..862
                     /locus_tag="KJL99_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            884..1231
                     /locus_tag="KJL99_003166"
     CDS             884..1231
                     /locus_tag="KJL99_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4437795.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDLPESPIGNNLEALAPGIEKLKQ
                     TSIQMVTLLNALQPSGKCIITGDFQKELAYLQNVILYNDSSLRLDFLGYNTQIIQRSD
                     NTCELTINEPLKN"
     gene            1400..2050
                     /locus_tag="KJL99_003167"
     CDS             1400..2050
                     /locus_tag="KJL99_003167"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4437796.1"
                     /translation="MANTILNHFKLDSTNFTNANMFQVNFVSSTQNATISWDYLLKIT
                     PVLISISDMYSEEKIKFVESCLNELGDITEEQLKIMRFAIIKSIPRATLTDKLENELT
                     KEIYKSSSKINNCLNRIKLPEMKEFSSKKTYDYIDTIIEDYENIKENAYLVVPQINYT
                     MDLNIEDSSSEELIPSRKTKILRTMALRSGNITHMKHITQRINILPERTIRIITTF"
     gene            complement(2671..3047)
                     /gene="rnpB"
                     /locus_tag="KJL99_003168"
     ncRNA           complement(2671..3047)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="KJL99_003168"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(3080..4225)
                     /gene="garK"
                     /locus_tag="KJL99_003169"
     CDS             complement(3080..4225)
                     /gene="garK"
                     /locus_tag="KJL99_003169"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HBD4437797.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNTG"
     gene            complement(4322..5212)
                     /gene="garR"
                     /locus_tag="KJL99_003170"
     CDS             complement(4322..5212)
                     /gene="garR"
                     /locus_tag="KJL99_003170"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HBD4437798.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALVCYYEKLAKVEVTR"
     gene            complement(5242..6012)
                     /gene="garL"
                     /locus_tag="KJL99_003171"
                     /pseudo
     CDS             complement(5242..6012)
                     /gene="garL"
                     /locus_tag="KJL99_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
     gene            complement(6028..7362)
                     /gene="garP"
                     /locus_tag="KJL99_003172"
     CDS             complement(6028..7362)
                     /gene="garP"
                     /locus_tag="KJL99_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HBD4437799.1"
                     /translation="MILDTVDEKRKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVICFVLTALWIKLIHNPTDHPRMSEEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            7737..9308
                     /gene="garD"
                     /locus_tag="KJL99_003173"
     CDS             7737..9308
                     /gene="garD"
                     /locus_tag="KJL99_003173"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HBD4437800.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMHCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMAPRTELANRWFDLMDINAGTIATGEETIEEEGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            complement(9360..10184)
                     /gene="agaR"
                     /locus_tag="KJL99_003174"
     CDS             complement(9360..10184)
                     /gene="agaR"
                     /locus_tag="KJL99_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HBD4437801.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKYTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLSRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGDHASSL"
     gene            10433..11713
                     /gene="kbaZ"
                     /locus_tag="KJL99_003175"
     CDS             10433..11713
                     /gene="kbaZ"
                     /locus_tag="KJL99_003175"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HBD4437802.1"
                     /translation="MKHLTEMVRQHKAGKTNAIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            11736..12209
                     /gene="agaV"
                     /locus_tag="KJL99_003176"
     CDS             11736..12209
                     /gene="agaV"
                     /locus_tag="KJL99_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HBD4437803.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            12220..12999
                     /locus_tag="KJL99_003177"
     CDS             12220..12999
                     /locus_tag="KJL99_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HBD4437804.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGDTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAATW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            12989..13798
                     /locus_tag="KJL99_003178"
     CDS             12989..13798
                     /locus_tag="KJL99_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HBD4437805.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDRSIAAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRG"
     gene            13885..14319
                     /locus_tag="KJL99_003179"
     CDS             13885..14319
                     /locus_tag="KJL99_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBD4437806.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSAALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            14316..>14729
                     /locus_tag="KJL99_003180"
     CDS             14316..>14729
                     /locus_tag="KJL99_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBD4437807.1"
                     /translation="MTHVLRARRLLTEEGWFDDHQLRVADGVIAAIEPIPVGVTERDA
                     ERLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHP"
BASE COUNT         3774 a         3762 c         3551 g         3642 t
ORIGIN      
        1 ttgattaacg tcaacttttt ctcttctgac aggacgtcat tttgtgaatg caatcgtttt
       61 ccataaattc ttctcccctc ataggcgacg aatagcattt tgtgttgagg atcacaaaac
      121 gaataattgc tggtcgccgc gataaggtca gacgaagtca acaaggggga ttttcactgt
      181 gctttacatc agcgtaggac acagaatgtt gcttcgttta ctgtaacgcc gggtaaatga
      241 gcgttttttg atagtgcgta agtgtcgtgc gaggaaaata agcatctatt attgtttgtc
      301 atgacgacaa ggcaggcatc aatgaaaggc ttcccaattg cacatatttt tcacccttca
      361 atcccgccaa tgcacgcagt ggttaacaat cacaatagaa atattgatta ttggacggta
      421 aaaagaaagt ttgcagaaat tgtctccacc aatgacgtta ataaaattta cagtataagt
      481 aatgaactcc ggagagtatt atctgcaatt actgcattga atttctatca aggcgatgtt
      541 ccttctgtca tgattccgaa tccaaccgga aaatatgagt ccattcatca tagatatttc
      601 tacaggagaa catgatgatt atatcataca aacattagat gtaggcactt ttgcaccttt
      661 tggtgaacaa tgtacttgct cagccgtcaa taaaaaagag ctggaatgta ttaaggagac
      721 gatttctaag tattgcgcaa agttcaccca aaaagaagcc atcttaacac cccctaggca
      781 ctttaataaa acctgcatta cttcagattg ttggcaaatt ctcttttttt cacccgatca
      841 ttttaataat gatttttatt gattatttat aaaggataaa ataatgttcc ctgtttcatc
      901 aattggtaat gatattagta gtgatttagt ccgtcgaaaa atgaatgacc ttcctgaaag
      961 ccccatagga aataacctcg aagcactagc accaggtata gaaaaactaa aacagacctc
     1021 tattcaaatg gtcactttac ttaacgcgtt acaaccaagt ggaaaatgca ttatcactgg
     1081 tgattttcaa aaagaattag cgtacttaca aaatgtaatt ctttataatg actcgtctct
     1141 tcgtctggat tttttaggtt ataacaccca aattattcaa cgatcggaca atacctgcga
     1201 acttaccatt aatgaaccgt taaaaaacta ggaaatatct acaggcaata tcaatgttaa
     1261 ttgcccatta aaagatattt acaatgaaat caggaggttg aacgtaatct ttagttgtgg
     1321 gactggagat atagttgatc tatcctctct ggacttacgt aatgtcgatt tagattatta
     1381 tgatttcaca gataaacata tggctaatac tattctaaat cattttaaat tggattcaac
     1441 aaattttact aatgccaaca tgtttcaggt taattttgtt agttcaacac aaaacgccac
     1501 aatctcctgg gattatttac taaaaataac gcctgtttta ataagcatta gcgatatgta
     1561 ttctgaagaa aaaatcaagt ttgtcgaaag ttgtttaaat gagcttggag acattaccga
     1621 agaacaatta aaaattatga gatttgcaat tataaaatct ataccaaggg caactcttac
     1681 agataaatta gaaaatgaat taacaaaaga aatatataaa agctcatcga aaatcaacaa
     1741 ttgcttgaac agaattaaat taccagagat gaaagaattc tcatcaaaaa aaacatatga
     1801 ttacatcgat acaatcattg aagattatga aaatatcaaa gaaaatgctt atctggtcgt
     1861 cccccaaatt aattatacta tggatttaaa catagaagac tctagctcag aagagttaat
     1921 accctcgaga aagacgaaaa ttctccggac aatggctttg aggtcgggga atataacaca
     1981 tatgaagcat ataactcaga gaatcaatat tttaccagag aggactatac gtataattac
     2041 gaccttttaa atgcaatata gacttaataa cttcttacaa agtctattat taataccgta
     2101 gatattcatc ttaccctgtg caccaaaagc aaaaacttat cgacgcgggt ttgaaactga
     2161 tggcaaccgc aaaaatgcct gatgcgctac gcttatcagg cctacgccat ctctgcaata
     2221 tattgaattt gcgtgctttt gtaggcagga taaggcgttc acgcagcatc cggcatgaat
     2281 aaagcgcact caacaatctg aaacccgccg gaacggttta ttacgtacat caggtgaaac
     2341 tgaccgataa gccgctttct tttgggtata gtgtcgtgga cagtcattca tctttctgcc
     2401 cctccaaaag caaaaacccg ccgaagcggg tttttacgta tatcaggtga aactgaccga
     2461 taagccgctt tcttttgggt atagcgtcgt ggacagtcat tcatctttct gcccctccaa
     2521 aagcaaaaac ccgccgaagc gggtttttac gtacatcagg tgaaactgac cgataagccg
     2581 ctttcttttg ggtatagcgt cgtggacagt cattcatctt tctgcccctc caaaagcaaa
     2641 aacccgccga agcgggtttt tacgtaaaac aggtgaaact gaccgataag ccgggttctg
     2701 tcgtggacag tcattcatct aggccagcaa tcgctcactg gctcaagcag cctacccggg
     2761 ttcagtacgg gccgtacctt atgaacccct atttggcctt gctccgggtg gagtttaccg
     2821 tgccacggac tgttaccagc cgcgcggtgc gctcttaccg caccctttca cccttacctg
     2881 atcccgcttg cacgggccat cggcggtttg ctctctgttg cactggtcgt gggtttcccc
     2941 cccaggcgtt acctggcacc ctgccctatg gagcccggac tttcctcccc tccacccgtc
     3001 tcccccgaag aggacgacga cgaagcggcg actgtctggt cagcttcggc gcgcagtata
     3061 gagggtttgc gcgcccttgt caccccgtgt tgcgcattcc aatcgccagt gtcgcggcaa
     3121 tattacgtga agcacggcag atattgtcat aagccccgcg gaatgcttcg tccaacgtac
     3181 ctatgctggt caatacgctg aagaccgcat caatgccatg ctgatgtaca acgccaacat
     3241 catcggtcag gctacccgca atgccaatca ccggtttatg gtacttcttc gccacgtttg
     3301 cgacgccaat cggtaccttg ccgtgaatac tctggctgtc aatacgccct tcaccggtga
     3361 tcaccagcgt acaatcgtga atatgttcct ccagattcag cgccgtagtg acgatttcaa
     3421 taccactttt cagttccgca ccaagaaacg ccatcagcgc cgcacccata ccgcccgcag
     3481 ctcctgcgcc ggggacatct tttacatcaa catgcagcgc ttttttaatg acatcggcat
     3541 agtgagagag gttattgtcc agctcaacaa tcatcgcttc actggctccc ttttgtgggc
     3601 caaagatgcg cgatgcgccg ttatcgccca ccagcggatt ggtgacatca caagcgacgc
     3661 gaatgacgca atcttttaag cgcggatcga ggccggaaat atcaatatca ttcagagtat
     3721 taagactacc gccgccaaaa ccaatttcat tgccgttggc gtcgcataat ttcgccccca
     3781 gcgcctgtac catgcctgcg ccgccatcat ttgtagcgct accgccaatg ccgataataa
     3841 tgtttgtcgc gccgctctcc agcgcctgca ggattaactc gcctgtgccg cgtgaagtgg
     3901 tcacgagtgg atcgcgtttt tccgcaggta ccagctccag cccactggcc gccgccattt
     3961 caataaacgc ggttttgcca tcgccggaga tcccccaact ggcattcact ttctcgccca
     4021 gcggccctgt aacccaggcg tgacgttcag aaccctgggt ggctgcaatc atcgcttcca
     4081 ccgttccttc gccaccgtcg gcaaccggaa tagaaacgta ctgtgcatca ggaaaaattt
     4141 cccgaaatcc tttttctatc gcctgcgcaa cctcgctggc agataaactt tctttataag
     4201 agtctggggc gattacgatt ttcataccta tgcctgttac cacatgacgc cggagggcgt
     4261 ttctcttatt cggcctggat tccaggcccg gattgcaata cgccatccgg gcacgacgtc
     4321 attaacgagt aacttcgact ttcgccagtt tttcgtagta gcacaccagg gcgctatgat
     4381 ccgccgttcc taaaccatct gctcgcagtg cctgcatcat ctccataacc gcagctgtga
     4441 gcggcagttg cgcgccgacg ccgtgggaag tatccagcgc atttgccaga tccttaatat
     4501 gcagatcaat acggaagccc ggcttgaagt tgcggtccat caccatcggc gctttggcat
     4561 ccagcacggt actgcccgcc agtccaccgc gaattgcctg atacaccagg tccgggttaa
     4621 cgcccgcttt agttgccagc gttaacgctt ctgacatcgc ggcaatattc agcgccacaa
     4681 tgacctgatt cgccagtttg gtgacgttac ctgcaccaat tgccccggta tgcaccacgg
     4741 aacccgccat tgctttcatc aaatcatagt atttgtcgaa aatagccttg tcgccgccca
     4801 ccatcactga cagcgtaccg tcgatggctt tcggttcacc gccgctcacc ggagcatcca
     4861 gcatatcaat gcctttcgct ttcagcgctt cgctgatttc acggcttgcc agcggtgcga
     4921 tagaactcat atcgatcaat accgtacctg gcttcgcgcc ttcaataatg ccattctcac
     4981 ccagcgccac ctctttcaca tgaggggagt ttggcagcat ggttatgatg acgtcgcact
     5041 gttcagcgat cgctttagcc gtagaggctg tttctgcacc tgcagcaatc acgtcagcaa
     5101 tagcttctgg gttacggtca gcaaccacca gcgagtaacc tgctttcaga aggtttttac
     5161 tcattggttt acccataatc cccaggccaa taaaaccaac tttcatagtc atatcaatca
     5221 tctctcttgt tgcggtggtg gttatttttt aaaggtatca gccagtttat gagtggcaga
     5281 gcggaagacg ccgagatcgc tgccgacagc cacaaacgtc gcgccccatt ccagataacg
     5341 acgcgcatcg gcttcgaccg gcgcgaggat accgctgggt ttgccgtgcg cgctggcacg
     5401 gttaaaaatg tgctgaattg ctttttgtac atccgggtgt gatgcattgc cgagatggcc
     5461 taatgccgcg gccagatcgc tggggccgac gaagatgccg tctacgcctt cggtagcggc
     5521 aatggcatcg acgttatcta cgccctgctg actttctatc tggaccagaa tagtgatgtt
     5581 cttgttcgac tgagcgaaat aatccgccac ggtgccaaac atattggcgc ggtgagaaac
     5641 ggagacgccg cgaatgcctt ccggtgggta acgggttgat gccaccgcct gctctgcttc
     5701 ctcttttgtt tctacaaaag gaatcaggaa gttatagaaa ccgatatcca gaagacgctt
     5761 aataatttcc ggctcgttgg tcggcactca cactactggc gcgctggcgc tgcctttcaa
     5821 ggccattaac tgcggaataa acgtggagat atcgtttggc gcatgttcgc catccagcac
     5881 cagccagtca aacccagcca aaccaagaac ttcagtgcta atcgggttag agagtgctga
     5941 ccagcaacca atttgtacct gtttcgcagc cagtgcggct ttgaatttat tcgggaaaac
     6001 atcgttattc atcgcttata cctttgctta tttctgcaat tccatacgtt taatgtcgcc
     6061 aactacgaag aggtagcaga ccatcgccat cagcgctgaa catcccacga aaaccagtgc
     6121 tgcattgaag gagtgcagtt cacttaccag gtagccaatc accagtggag tgacaatgga
     6181 ggcaacattg ccaaagacgt taaagacgcc gccacagagg ccaacaatct ctttcggcgc
     6241 ggtgtcagaa atcaccggcc agcccagagc accaaatcct ttgccaaaaa aagccagcgc
     6301 catcagcatg accaccagcg tggtgttgtt ggtgtagtta cataagatga tggtggaagc
     6361 cagcaacatt cccagcacaa tcggtagctt acgtgccagg gtcagggata aaccgcgttt
     6421 gatcagataa tccgagaaga cacctcccag cacgccgccc gcaaaaccac acagtgccgg
     6481 aatcgaggcg accagaccca ctttcagaat cgacatgcct ttttcctgca ccagataaat
     6541 cgggaaccag gtgaggaaga accaggtgat ggtgttgata aaatattgtc cgaaaaatac
     6601 gcccaacatc atgcggttag agagcaattg cttgatgtaa tgcagtttgg gaccgcttgc
     6661 tgccgcgctg cccggctttt tgtggtccat atcgaccacc gcgccatttt cagagataaa
     6721 cttcagctct tcctcagaca tacgtgggtg atctgtcggg ttatgaatca acttgatcca
     6781 cagcgccgtc agcacaaaac aaatcacccc cataacggta aagacgtgct cccatcccca
     6841 ggcgaaagtc agccagccaa gcagaggcga aaagagcgcc agcgagaaat attgtgccga
     6901 gttaaagatg gcggaggcag taccacgttc tttcgtcggg aaccaggcgg cgacaatacg
     6961 ggcgttcgcc gggaaggatg gagcttccga gaatcccagc ataaagcgca taaagaacat
     7021 ggagatccct gcccaagcca gcgggaacat atcaacaaag ccttgcagga aggtgaacag
     7081 cgaccagaaa aagaggctgt aggtgtaaac ttttttcgag ccaaacttat caagcaacca
     7141 gccgccgggg atttgcatca gcaagtaggc ccagccaaaa gcggagaaga tgtaacccat
     7201 cgaaaccgca cttaactgca actcttttgc cacttcggta ccagcaatag acagcgttgc
     7261 acgatcggcg tagttaacgg cggtaacaat aaaaataatc agtaatatta aatagcgggt
     7321 atgcacacct ttcctttttt cgtcaacggt gtccagaatc attttattta cctcgggtac
     7381 ttatgctgat ttttattatt atggggaagg tgttatttat aagtttcatt tatgccgtaa
     7441 cgacaatgaa ctcgggaatt agtataagca gcgcgagaat aataatcatt gtgcaaatgc
     7501 taatttaatt aatacttttt aaatattatt ttgagcatat gcacataagg ttgcgcgcta
     7561 aagcacagat ttgcgcttta ccttgccggg cggcactgca atccctaaaa tgattgacat
     7621 tgatcacatt tctgcgttta aactcctgac attcttattt cacccaatga aatcatttat
     7681 ttttaaatga gaccaggtcc tcattttaat aacccctggc tggagaatat tgcacaatgg
     7741 ccaacatcga aatcagacaa gaaacgccaa ctgcgtttta tataaaagtt cacgacacag
     7801 ataatgtggc aattattgtt aatgataatg gcctgaaagc aggaacgcgt tttccggatg
     7861 ggctggaatt aattgaacat attccccagg ggcataaagt cgcattgctg gacattccgg
     7921 ctaatggtga aattattcgt tatggcgaag tgattggtta cgccgtgcgt gcaatcccac
     7981 gcggaagctg gatcgacgaa tcaatggttg tactaccgga agcgccgccg ttacacacgc
     8041 tgccactggc aaccaaagtc ccggaaccct taccgccgct ggaaggatac acctttgagg
     8101 gctatcgcaa tgccgatggc agcgtgggca ccaaaaacct gctcggcatc accaccagcg
     8161 tccactgtgt ggcaggtgtg gtggactatg tagtaaaaat cattgaacgc gatctgctac
     8221 cgaaataccc gaacgtcgat ggcgtggtgg ggctgaatca tttgtacggt tgtggcgtgg
     8281 cgattaacgc accggcagca gttgtaccta ttcgtaccat tcacaatatt tcgctgaacc
     8341 ctaactttgg cggcgaagta atggtgattg gcttgggttg tgaaaagttg cagcctgagc
     8401 gcctgctgac tggaacggat gatgtgcaag ctattccagt agaaagcgcc agcattgtca
     8461 gtttgcagga tgaaaagcat gtcggttttc agtccatggt cgaggatatt ttgcaggtcg
     8521 ccgaacgcca tctacaaaaa ctgaatcaac gtcagcgaga aacctgtccg gcttcagaac
     8581 tggttgtcgg catgcattgc ggtggcagcg atgcgttttc tggcgtaacg gcaaacccgg
     8641 cggttggcta tgcgtctgat ctactggtgc gctgcggcgc aacggtgatg ttttcagaag
     8701 tcacggaagt gcgtgacgcg atccatctgc tgacaccacg cgcagtgaac gaagaggtcg
     8761 gcaaacggct gctggaggag atggagtggt acgataacta tctcaatatg ggaaaaaccg
     8821 accgcagcgc caacccttcg ccgggcaaca agaaaggcgg tctggcaaac gtggtagaga
     8881 aagcgctcgg ctccattgct aaatcgggca aaagtgcaat tgttgaagtg ctgtcgcccg
     8941 gtcaacgccc gactaaacgc ggattaattt acgccgcgac gccagccagc gattttgtct
     9001 gtggcacgca acaggtggct tcgggtatca ccgtgcaagt gtttacgacc ggtcgtggta
     9061 cgccgtacgg cctgatggcg gtacccgtca ttaaaatggc accccgcacc gagctggcga
     9121 accgctggtt tgatttaatg gatattaatg cgggcaccat cgctaccggc gaagaaacta
     9181 ttgaagagga gggctggaag ttgttccact ttattctcga cgtcgccagc gggaagaaga
     9241 aaaccttctc ggatcaatgg ggactgcata accagctggc ggtgtttaac ccagcaccgg
     9301 tgacctgata attcaattcg tattatgccc gatagcacac gtttatcggg catattgagt
     9361 cacagcgaag acgcgtgatc cccgaccaga atcacttcaa ccccagcctt tcgcagtcct
     9421 tccagactat ccgcaggaat gccttcatca acaatgatca tgtcgatacg ttgagtatca
     9481 atgatcttat gtaaactgga acgattgaat ttactggaat cggtgaccac gatgatccgt
     9541 tccgcaactt cgcacatccg acggcttaaa cgggcttcat cttcattatg tgtgctgacg
     9601 ccgcgctcca gatcgatcgc atctacacca agaaacagca tatcgaagtg gtaattttgc
     9661 agcgattgct cagcctgatc gccgtaaaaa gattgcgact gacggcgcaa atgcccgccg
     9721 gtcatcagca gctcaacgcc ttccgcttcc agcaacgcat tagccacgtt cataccgttg
     9781 gtcatcgcaa ttacgtcagt gtacttgcgc atcagacgag caatctcaaa agtggtggtc
     9841 ccggaatcga ggatcacccg atgacctggc tgaatcaact caacggcagc tttcgcaacg
     9901 ctgcgtttca tcgcggtgtt cagtgcgctt ttatcttcca ctgatggctc gactgacggc
     9961 gtcgtgctat cgcagatcaa cgcgccacca taggcacgca cagcgatccc ctgcttttcc
    10021 agaaacgcca gatcattgcg gatcgtcaca gtagatacgc catacaatgc cgacagatcg
    10081 ttaacctgca cactcccttg ctgtcgcaga cgctgaatga tctgttctcg tcgctcgctg
    10141 gtgcctgtca ctcgcttctc acctgaagcg tcggtattac tcatagtaag tcctttcgta
    10201 aaactttcgt ttcatttcgt tttgcctatt aacgcctttc tattaagcaa atgcaagccc
    10261 accttgccca ttggcgcaag ctactctcgt ttcagtgact ttcattacgt ttcttttgtg
    10321 aatcagatca gaaaactatt atctttcgtt ttatttttat ctcaccatga cgcagtatca
    10381 attgaaacaa aacgaaagat caatatcgca gtaatctgaa ctggagagga aagtgaaaca
    10441 tctgacagaa atggtgagac agcacaaagc gggcaaaaca aatgcaattt atgccgtttg
    10501 ttccgcacat ccgctggtgc tggaggctgc aatccgctac gccagtgcaa accaaacgcc
    10561 gttactgatt gaagcaacct ccaatcaggt agaccagttc ggcggttata ccggaatgac
    10621 gcccgccgat tttcgcggct ttgtttgtca gctcgccgac tcgttgaatt tcccgcagga
    10681 tgcgctgatt ctgggtggtg accatctggg gccaaaccgc tggcaaaacc tgccagccgc
    10741 tcaggcaatg gccaatgccg atgatttgat taaaagctac gttgcggcag gattcaaaaa
    10801 aatccacctt gattgcagca tgtcctgtca ggacgatcca attcccttaa ctgatgacat
    10861 cgtggctgaa cgcgccgccc gtctggcgaa agtggcggaa gaaacctgtc gcgaacactt
    10921 tggcgaagcc gatctggagt atgtcattgg taccgaagtg ccggtacctg gcggcgcgca
    10981 tgaaacctta agcgagctgg cggtcaccac gccggatgcc gcccgcgcca cgctggaagc
    11041 ccatcgtcac gcctttgaaa agcaaggttt gaatgccatc tggccacgca ttattgccct
    11101 ggtggttcaa cccggcgtcg aattcgatca caccaacgtt attgattatc agcccgccaa
    11161 agcgagcgcc ttaagccaga tggtcgaaaa ctacgaaacg ctgattttcg aagcgcactc
    11221 taccgattat caaacgccgc aatctctgcg ccagctggtg attgaccact ttgccattct
    11281 gaaagttggc ccggcgctga ccttcgccct gcgtgaagcc cttttttctc tggcggcgat
    11341 tgaagaagaa ctggtaccag cgaaagcctg ttccggtctg cgtcaggtgc tggaaaacgt
    11401 gatgctcgac cgcccggaat actggcaaag ccactaccac ggtgacggca acgcgcgtcg
    11461 tctggcgcgt ggttacagct actcagatcg cgtgcgctat tactggccgg acagccagat
    11521 tgatgacgct ttcgctcatc tggttcgtaa tctggcggat tcaccaattc cgctaccgct
    11581 catcagccag tatctgccgc tgcagtacgt gaaagttcgc tccggcgagc tgcaaccgac
    11641 gccacgggaa ctcattataa accatattca ggacatcctg gcgcagtacc acacagcctg
    11701 tgaaggccaa taagcaaaac aaagaggaac atgctatgcc aaatattgtt ttaagccgga
    11761 ttgatgaacg cttgattcac ggtcaggtcg gcgttcagtg ggtcggattt gcgggggcaa
    11821 atctggtgct ggtagccaac gatgaggttg ccgaagatcc ggtacaacaa aacctgatgg
    11881 aaatggtact ggcagaaggg atcgccgtac gtttctggac gctgcaaaaa gttatcgaca
    11941 acattcatcg cgccgccgat cgacagaaaa tcctgctggt ttgtaaaaca cccgccgatt
    12001 tcctgacgct ggtgaaaggt ggcgttccgg tgaatcgcat taacgttggc aatatgcact
    12061 acgccaatgg caaacaacaa atcgccaaaa cggtttctgt ggatgcgggc gatatcgcag
    12121 catttaacga cctgaaagcc gctggggtgg aatgcttcgt tcagggcgtc ccgacagagc
    12181 ctgctgtgga cctctttaaa ttactttgag ggattcatca tggaaatcag cctgttgcag
    12241 gcatttgcgt tgggcattat cgcctttatc gctggcctag atatgtttaa cggcttaacc
    12301 catatgcacc gcccggtggt cctcggcccg ttggtcgggc tggtacttgg cgatctgcat
    12361 accggaattt taaccggcga tacgctggaa ctagtgtgga tggggctggc cccgctggcg
    12421 ggcgcacagc cgcctaacgt gattatcggt actatcgtcg gcacggcgtt tgccattact
    12481 actggcgtga aacccgatgt cgcggtcggt gtcgccgtac ctttcgctgt cgcagtacag
    12541 atggggatta ccttcctgtt ctcggtgatg tctggcgtga tgtctcgctg cgaccggatg
    12601 gcggagaacg ccgatactcg cggcattgaa cgcgtgaact atctggcact gctggcactc
    12661 ggcactttct attttctctg cgctttcctg cctatctact tcggcgcgga acatgcaaaa
    12721 accatcattg atgtcctgcc gcaacgatta atcgacggcc tcggcgtcgc gggcggcatc
    12781 atgccagcaa tcggttttgc cgtgctgctg aaaatcatga tgaaaaacgt ctacataccc
    12841 tacttcatcc tgggctttgt tgccgcaacc tggctcaagt taccggtgct ggctattgct
    12901 gccgccgcgc tggcgatggc gctgatcgat ctgctgcgta aatctcctga accgactcaa
    12961 cctgcggcac agaaagagga atttgaagat ggcatctaat caaaccaccc tgccgaacgt
    13021 cagcgaaaat gaagaaacac tgctgactgg cgtcaatgaa aacgtgtatg aagatcggag
    13081 cattgccgcg gagctgacga aaaaagatat caaccgcgtc gcctggcgtt ccatgctgtt
    13141 acaagcgtct tttaactacg aacgtatgca ggcttctggc tggttgtacg gtctgctgcc
    13201 tgctctgaaa aagatccaca ctaataaacg cgacctggcg cgcgccatga aggggcatat
    13261 gggtttcttc aatacccatc cgtttctggt gacgtttgtt atcggcatta tccttgcgat
    13321 ggagcgttct aagcaggacg ttaacagtat tcagagcacc aaaattgccg tcggtgcgcc
    13381 gcttggcggg attggcgatg cgatgttctg gctaacgcta ctgccgattt gtggcgggat
    13441 aggcgcaagc ctcgcgctac aaggctctat tcttggcgca gtcgtcttta ttgtgctgtt
    13501 caacgtggta cacttggggc tgcgttttgg tctggcgcat tatgcttacc gcatgggcgt
    13561 ggcggcgatt ccccttatta aagcgaatac caaaaaagtc ggccatgctg cgtcaatcgt
    13621 tggaatgacg gtaatcggcg cactggtggc aacctatgtt cgtttaagca ccacgctgga
    13681 aatcacggcg ggcgacgcag tagttaagtt acaggctgat gttatcgaca aactgatgcc
    13741 tgccttctta ccgctggtct acaccctgac catgttctgg ctggtacgcc gcggctagag
    13801 tccgctgcgc ctgattgcgg ttaccgtggt tctagggatc gtcggtaaat tctgccattt
    13861 cctttaaata caatgaggta ttagatgtta agtattattt tgacagggca tggcgggttt
    13921 gccagtggca tggaaaaagc aatgaagcaa atcctcggcg atcaatcgca gtttattgcc
    13981 atcgattttc ccgaaacctc aagcgccgcg ctgcttacat cgcagcttga agaggccatc
    14041 gcccaactgg attgtgaaga cggcattgtt tttctgacgg atttgctggg cggcacgccg
    14101 tttcgcgtag cttcaacgct ggcgatgcaa aaaccgggct gtgaagtgat taccggcacc
    14161 aatttgcaac tgctactgga gatggtgctg gagcgcgaag ggttaagcgg tgaagaattc
    14221 cgtgtacagg cgctggagtg tggacaccga gggctgacca gtctggtgga cgagttaggc
    14281 cgctgccatg aagaatgtcc ggtcgaggaa ggaatatgac acacgttctg cgcgccagaa
    14341 ggctgctgac tgaagaggga tggttcgatg accatcagtt gcgtgttgct gacggtgtca
    14401 tcgcagcaat cgaaccgatt ccagtgggcg tgactgaacg cgatgcggaa cggctctgcc
    14461 ccgcttacat cgacacccat gtacacggtg gtgcgggcgt tgatgtaatg gatgacgcgc
    14521 cggatgtact cgacaagctg gcaatgcaca aggcacgcga aggtgtcggc agttggttac
    14581 cgaccaccgt aaccgcgccg cttaatacca ttcatgcggc gctgaaacgt attgctcaac
    14641 gttgccaacg cggcggacct ggtgcgcaag tgctggggag ttatctcgaa ggaccgtact
    14701 tcacgccgca gaataaaggc gcgcatccg
//
LOCUS       DAEBXN010000099        14706 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.099, whole genome shotgun sequence.
ACCESSION   DAEBXN010000099 DAEBXN010000000
VERSION     DAEBXN010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14706
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.099"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(10..1272)
                     /locus_tag="KJL99_003181"
     CDS             complement(10..1272)
                     /locus_tag="KJL99_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HBD4437808.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGIDPCEQKRVKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALELSRLPELLSRIDG
                     YKGQPVTRLAVMLNLLVFIRSSELRYARWSEIDIDNSMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQVVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TQEVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANREKGISPFEYAKINNPLK"
     gene            complement(1470..1545)
                     /locus_tag="KJL99_003182"
     tRNA            complement(1470..1545)
                     /locus_tag="KJL99_003182"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1510..1512),aa:Phe,seq:gaa)
     gene            complement(1651..2358)
                     /locus_tag="KJL99_003183"
     CDS             complement(1651..2358)
                     /locus_tag="KJL99_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HBD4437809.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLITPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            2756..4891
                     /gene="speC"
                     /locus_tag="KJL99_003184"
     CDS             2756..4891
                     /gene="speC"
                     /locus_tag="KJL99_003184"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HBD4437810.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGTTPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LTIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFISMMADSSPLLLELNE
                     NDPGIFVTQLVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSSLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(4940..6196)
                     /gene="nupG"
                     /locus_tag="KJL99_003185"
     CDS             complement(4940..6196)
                     /gene="nupG"
                     /locus_tag="KJL99_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HBD4437811.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(6398..7477)
                     /gene="mltC"
                     /locus_tag="KJL99_003186"
     CDS             complement(6398..7477)
                     /gene="mltC"
                     /locus_tag="KJL99_003186"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HBD4437812.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQNTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(7542..7817)
                     /locus_tag="KJL99_003187"
     CDS             complement(7542..7817)
                     /locus_tag="KJL99_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HBD4437813.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(7845..8897)
                     /gene="mutY"
                     /locus_tag="KJL99_003188"
     CDS             complement(7845..8897)
                     /gene="mutY"
                     /locus_tag="KJL99_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HBD4437814.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMAHFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            9058..9777
                     /gene="trmB"
                     /locus_tag="KJL99_003189"
     CDS             9058..9777
                     /gene="trmB"
                     /locus_tag="KJL99_003189"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HBD4437815.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            9777..10103
                     /locus_tag="KJL99_003190"
     CDS             9777..10103
                     /locus_tag="KJL99_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HBD4437816.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKIVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEECKLDEVRTSEL
                     FDVWWD"
     gene            10287..11006
                     /locus_tag="KJL99_003191"
     CDS             10287..11006
                     /locus_tag="KJL99_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HBD4437817.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGDSSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            11182..12228
                     /gene="ansB"
                     /locus_tag="KJL99_003192"
     CDS             11182..12228
                     /gene="ansB"
                     /locus_tag="KJL99_003192"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HBD4437818.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINADCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            12345..13307
                     /locus_tag="KJL99_003193"
     CDS             12345..13307
                     /locus_tag="KJL99_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HBD4437819.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAISLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANER"
     gene            complement(13408..>14706)
                     /locus_tag="KJL99_003194"
     CDS             complement(13408..>14706)
                     /locus_tag="KJL99_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HBD4437820.1"
                     /translation="DSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIAA
                     ITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVNV
                     LANMMFGSISGSAVAAAAAVGGTLNPIQTKEGYDPAFSTAVNVSSCITGLLIPPSNVL
                     IVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKAF
                     LDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRHLPKLILES
                     VVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGIF
                     MDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISGV
                     KIQHLIKPLLLFYAALLVALMMITYIPQISLFIPQLLGLM"
BASE COUNT         3836 a         3550 c         3791 g         3529 t
ORIGIN      
        1 gactgcttac tattttaatg gattgttaat ctttgcatat tcaaacgggc tgataccctt
       61 ctcccggttc gcatctagaa aatcggccca ccattgcaac atcagtcgcc gttcttccag
      121 atgttctgct ttatggatat acgcagcacg tacaccatta cgctcctgat ggctcatctg
      181 acgttccaca gcatcgcggg accacaaacc tgattcaatc aatgcactgc acgccatcgc
      241 ccggaatcca tggccacaga cttcctgggt tgtgtcatac cccatcaccc tcagggcttt
      301 atttacagta ttttcactga ttggtttacg cgggtcatgt gcacccgtaa agatcagacc
      361 attttcacca gcccatgtct gaagttctgc cagtatggct acaacctgtt tgctgagtgg
      421 cacaagatgt ggtgttcgca tcttggatcc ccggtgtgag aattttacgc ctggcagagg
      481 ttcgcgttcg gctggaatag tccacatgga attgtcaata tcaatttccg accagcgggc
      541 atatctgagt tcactggaac gaataaaaac cagtaaattc agcataactg ccagtcgggt
      601 gacaggctgg cctttataac cgtcaatacg cgacagaagt tcaggcaggc gtgaaagctc
      661 cagagcaggg cgatgctggc gttttaccgt ggtcaatgca ccagccatat ccagagccgg
      721 gttatatctg atgatgccag actgtacggc gtaacgcatt acagcagata ttctttgctg
      781 cagacgactg gcgatttcat aaatttgttt tgcttctgca gcacgaacag ggataagcag
      841 gtccggggta ttgagtgttg tgatatcccg gttgccgata aaggggaaaa cgtgtgtctc
      901 gaggcttttg agtactttat cgctgtgtga ttgtgcccat tgtttattac tggcatgcca
      961 gcgtcgtgca atatgttcaa aagagagctg taaatcaggg acaacttttt taactctttt
     1021 ctgttcgcaa gggtcaatcc cctctgaaat cagttttcgg gcctcatccc gttttgtacg
     1081 agcttcggag agagaaactt ctggatacac accaagtgcc agagtcttct ccttacccag
     1141 aatacgataa cggagacgcc agtaacggga accattagga tgaaccagca ggaacatgcc
     1201 ggccccgtca gtgagtttat aagccttgtc agtgggcttt gcagcccgga tttttgcgtc
     1261 agtcagtgcc atagttcagc cttctgaatg cgttaaagag ggtacaaatt ctgtcgaact
     1321 ggggtatacc cgcaattata cccacacaat ggggttgatg tggggggaat ctggttgagt
     1381 tcggtagaat tgatttggag gaagaacact taaatcatgg tgttctgaaa cgaaaaacgg
     1441 acctccgtgg aggtccgttg atatgaattt ggtgcccgga ctcggaatcg aaccaaggac
     1501 acggggattt tcaatcccct gctctaccga ctgagctatc cgggcaacgg ggcgcattaa
     1561 acctgattcg cctcgtctcg tcaatcaaat ttcttcaatt tactgcagac tgcacaatct
     1621 atcatcactt tgccgctatt gcacgcattg tcaggcaaac caggcggtcc aggcggcgga
     1681 aagcggcatt gccagaagga gtgctggaag catgttgacc accgggaaca ttttaatgcc
     1741 acagatgcgt aatccggttg ccagcagcaa taaaccgcct acggcgctga agtctgccat
     1801 catcgacggt gtgatcagcg gtaatatcag cgcggcagcc cacgccagcg ttaactggat
     1861 gatcagtaat gggatactaa ttaccgacac cgcaataccc agcgagcagg cgaagatcat
     1921 cgccgtaaag aaatcaagaa atgacttggc gattaaaata ctcggatcgc cggtcatccc
     1981 ttcgttcatc gccccgaaga tcccggtgcc gctggcgcaa aacaggacaa taatcgcgac
     2041 ataattctga ataaaagatt catgcgctgg cttcttacgt gagtgacgaa acagattttg
     2101 tgctttggcg actgctgtat tgacaccttt ctccagcaaa caaatttcgc caattagagc
     2161 gccaagtagc gtagctaaaa ccatcgctgg aaggttggcg catttcacaa ccagtaaaat
     2221 accaatcccc agcgatgcca gaccaaaaat agatgtcatg gagacgcgga tacgttccgg
     2281 taagcgttgg ctgagcagtg cgccgagaac gccacccagt aagactgcac tagcgttgat
     2341 aaaagggccg atgaccacaa gagttcctgt tagctgtatt gtttgatttt cttattatga
     2401 actttttgcc ttgttgatgg cttgtctggt cgcaaagtcc tggcttgcac gccttagcga
     2461 aaggtgccat gattgcgcga attttctcct ctctgtacgg agtttgcccg atgcacgcca
     2521 tctccttaca ttctctcgct tatcgccgtt tcgcgcgaaa cgtttccctt tctatgttac
     2581 tgctcatgcg gtgaagttaa cgcacgctca ctgcaggaca acagtaaaat cagagcgttt
     2641 ctgcttttac tgatgtctgg cggtcggagc tggtgaccag tttgacccat atctcatggg
     2701 gcagggtttt ccaccttgtc cggtattctt acttccccga aacgggtttg cgcttatgaa
     2761 atcaatgaat attgccgcca gtagtgaact ggtatcccga ctttcttctc atcgccgcgt
     2821 ggtggcgttg ggagatactg attttacgga cgtcgcggca gtcgtcatta ccgctgcgga
     2881 tagtcgcagt ggcattcttg cgttgcttaa gcgcaccggt tttcatctac cggtgttttt
     2941 gtattccgaa catgctgttg aattacctgc gggcgttacg gcggtaatca acggcaacga
     3001 gcagcagtgg ctggagctgg aatccgcagc ctgtcagtat gaagagaatt tgctgccacc
     3061 gttttatgac acgctgacgc agtacgttga gatgggcaac agcacctttg cttgccctgg
     3121 acatcaacat ggtgcgttct ttaaaaagca tcctgccgga cgccattttt acgatttctt
     3181 tggtgagaac gtctttcgcg ccgatatgtg taacgctgac gtaaaattgg gcgatctgct
     3241 tattcatgaa ggatcggcga aagatgcgca gaaattcgca gccaaagtct ttcatgccga
     3301 taaaacctat tttgtgctga acggcacatc ggcagcgaat aaagtggtga cgaatgcgct
     3361 gttaacgcgt ggcgatctgg tgctcttcga ccgtaacaac cataagtcga atcatcacgg
     3421 cgcgctgatt caggcgggga cgacgccggt ctatctggaa gcttcacgta acccgtttgg
     3481 tttcattggc ggtattgatg cgcactgttt taatgaagag tatctgcgcc agcaaattcg
     3541 cgacgttgcg ccagaaaaag ccgacctgcc gcgcccgttt cgcctgacga ttattcagct
     3601 gggaacctat gacggcactg tctataacgc ccgtcaggtg atcgatacca ttgggcatct
     3661 gtgtgattac attctgtttg attccgcgtg ggtcggttac gaacagttta tctcgatgat
     3721 ggcggatagc tcgccgctgc tgttagaact taacgaaaac gatccgggga tttttgtgac
     3781 ccagttggtg cacaaacagc aggctggatt ctcacagacg tcgcagatcc ataaaaaaga
     3841 taaccatatc cgcggacagg cgcgtttttg cccgcataag cggttgaata acgcctttat
     3901 gctccatgct tctaccagcc ctttctatcc gctgtttgct gcactggatg ttaacgccaa
     3961 aattcatgaa ggggagagtg ggcgtcggct gtgggctgag tgcgttgagt tgggtattga
     4021 gtcgcgcaag gctattcttg cgcgctgtaa gctgttccgc ccgtttatcc cgcccgttgt
     4081 tgatggcaaa ttgtggcagg attatccgac atcagtgtta gccagcgacc gccgtttttt
     4141 cagttttgag ccgggggcga agtggcacgg ctttgaagga tatgccgcgg atcagtattt
     4201 tgttgatccg tgcaagctgt tactcactac accaggtatc aatgccgaaa ccggcgaata
     4261 tagcgacttt ggcgttccgg cgacgattct ggcgcactat ctgcgtgaga acggcattgt
     4321 gccggagaag tgcgatctca actccattct gttcttatta actccggcgg aaagccacga
     4381 gaagctggca caactggtgg cgatgctggc gcaatttgaa cagcatattg aggatgactc
     4441 gtcgctggct gaggtgttgc cgagcgttta taacaaatat ccggtgcgct atcgcgacta
     4501 caccctgcgc cagttgtgtc aggagatgca cgatctgtat gtcagtttcg acgtcaaaga
     4561 cctacaaaaa gcgatgttcc gccagcagag tttcccgtca gtggtgatga acccccagga
     4621 tgcgcatagc gcttatattc gcggtgaagt ggagttggtg cggattcgtg atgccgaagg
     4681 gcgaattgcg gcagaagggg cgttgcctta tccacctggc gtgctttgcg tggtacccgg
     4741 ggaagtctgg ggtggggcgg ttcaacgtta tttccttgcg ctggaagaag gcgtgaattt
     4801 gttgccggga ttttcgccgg agttgcaagg tgtctatagc gaaaccgatg cggatggcat
     4861 gaaacgattg tacggttatg tgttgaagta agaataaaaa aacgggtcac cttctggcga
     4921 cccgtttttc tttgcgtaat tagtggctaa ccgtctgtgt gcctgtcggg acacgaacgt
     4981 gtttatattt gaacatcgcc atgaacgcga aggccagaac cacggaataa ccagcgaaaa
     5041 tcaaccatac ggtctgccag tcggtaatgc cgttttgggt gtacatctca acaactttac
     5101 cgctcacgat gccgccgagg atacagccga agccgttagt catcatcagg aacatccctt
     5161 gtgcactggc gcgaattgcc gggctaactt ctttttcgac aaacaccgaa ccagagatgt
     5221 tgaagaagtc gaatgcgcaa ccgtaaacga tcatcgacag taccagcagt acagtaccga
     5281 acggagtcgg gtcgccgtaa gcaaacagcg caaaacgcag gatccacgcc acaatactga
     5341 tcatcattac gttcttaata ccgtagcggc ttaagaagaa cgggatggtc agaatgaaca
     5401 gggtttcaga gatctgcgaa atcgacatga tgattgacgc atgctgcacg atgaagctgc
     5461 tggcaaacat cggatctttg tcgaagctgt gcaggaaggt attaccgaac atgttggtaa
     5521 tctgcagttc cgcgcccagc agcattgaga agatgaagaa gattgccata cgcttgtttt
     5581 taaacagcgc gaatgcatcg aggcccagca gggttgtcca gctctgattc gcttgctgtt
     5641 tagcaaccgg aatatgcggc agagtcaggg taaacagaac cagaatggcg gaaagtgctg
     5701 cgccaatata cagctgcatg tggcttaatt cgaagccaga caggctcacc acccacattg
     5761 ccatgataaa gccgatggtg ccccagatac ggattggcgg gaagtcagta acgatatcca
     5821 tcccggcatt ttgcaggcga tagtaagaga tggtgttgat taacccaagc gttggcatat
     5881 aagcaaacga gttaatcaat atcacaagga acatcgcttc cggcgtcgtg acctgtgccg
     5941 ccatgaacag cgtgatagcg ccaatggtgt ggcaaatggc atatacccat ttcgcactta
     6001 accatttgtc ggccacaatc cccagcagcg caggcataaa taccgcggcg atacccagtg
     6061 agctataaac tgcgccaata gaagcaccgt caaacttcag ggtaacaaac atataggagc
     6121 cgagggtcgt cagccaactt ccccacagac agaactgcag aaaagagagg attttcagct
     6181 gcagcttaag attcatgtta atttcctcac atcgtgatgc ggatgagtgt ttgaaaaagc
     6241 acatttgacc gtgcgagatg atgggattct atcaacttcg cggactggtt ttttgttacc
     6301 tgtggcaaat aattgcaaac attcccaatt tgcaaaacga aattgtgatg tggataacat
     6361 ttttgcccct gagcatcgtc aggggcggtt aatggaatta tcggcggcgg taggattttt
     6421 gcgcggtatt cactttataa agataacggc gagattccgc agagggatgg cgggtcgtca
     6481 gcgtctgata aacatcgccc ggcgtcatgg tgttaataat attggcagcc tgaatcttat
     6541 cattcgaaaa gactcgcagc acgctgcctg cgccgccgtt ataggcggtg atgacggcat
     6601 aacgccgcga tgttgggtta tcaattccgc cgagataaac attgttcagc atcgccagat
     6661 acgcggtgcc ggtatcaata ttgctggcag gatcaaacaa gaaactgcgg ctcggcgtgc
     6721 cggatttccc ctgcgagcgg aacacatctt tcccggcagt attttgtacc acctgcatta
     6781 atcccagcgc atcggaacgg ctgaccgcat acgggttaaa ggaagattca gtctgcataa
     6841 ttgccagaat cagcgactca tcaacgccat atttccgtga cgcctggcgg accatgccga
     6901 gatatttgtg cgcacgttta tcaaggtggt tcggcaccat gttaatggtg acgctgtaga
     6961 tgatacgtag cccgttgctg cggcttttca gacggttttt cagcagataa tccgcgaagt
     7021 tgcttgcgcg accttcccag cgaatcggct gcccggtgtt gtccaccacc tgaccgtaaa
     7081 ggaaaggttc tttcgaaatt gtaatatcat caacgtcgga atagagatcg accgaactcg
     7141 gatcgtcacc catcagcaac gttttgataa ttgcccggcg caaatgcgcg gcaggttctg
     7201 tcccggcgat ggtttcgata gtaatcgtac cgtcatcgaa gttgatgtgg ctgcgggtct
     7261 gatattgatc ggtgtatttc acgtagtcct taggaccggc gatcaccacc tctttgaagc
     7321 cccagatgtt ctcaatattg tgggcaaatt gccccatcag aatatcaaaa ccgttggtat
     7381 ctttgaccca ggcttcgtta taggtatcgc cttttttggt cgtcgaacag gagatgagca
     7441 acggcgcaat caaagccagc gcgagatatt ttttcatcat tccgggtgcg tgttgtgttt
     7501 gtttttatgc aagctgccgg aggcgtgctc cggcactgtt tttatttttt atcttccggc
     7561 gtatagccct cgatatgcac ctctttaccc tcgaacagga agttgaccat ctcctgctcc
     7621 agcagcttgc ggtgctcggc attcatcatg ttgagtttct tttcattaat cagcatggtt
     7681 tgtttgtgct gccactgcgc ccaggcttct ttggagatct cgttatagat gcgttttccc
     7741 agctcgccgg ggtacagctg aaaatcctga ccttctgctt cacgttgcag gaaagtacaa
     7801 aaaatcgttc tgctcataaa tcatcctctt tatcgactca cgcgctaaac cggcgcgcca
     7861 gtgcgtaact gctgtaacaa acgctccacg ggagccgcca ggccaactga cggcggttgc
     7921 gctaagttat accagagcgc attgccttca tccatgcagc cggtgaatga cgacacggga
     7981 agccacatag gcacaatatc taagtggaaa tggctgaagg tatggcgaaa cgcggtcagt
     8041 tgcgtcaggt tatcggcagc aatctgccgt tgtgccagcc aatgccgcaa actttcttcg
     8101 tcggcaaact gcgggaaaca gtataaaccg ccccacaatc cgctcggcgg acgctgcgcc
     8161 agcaatactt catcttcgtg ctgtaacagg agaaagtagc cggtgcgctc cggcagcgtc
     8221 tgtttcggtt ttttgcccgg ataaagcgac cagctattgt tggtggcggc aatacatccg
     8281 ttttgtagcg gacagagcga acatttcggc ttcgaacgcg tgcaaatcat cgcgcccaaa
     8341 tccatcatcg cctgattaaa ccgttccacg ccgaccgcgg gcgtcacctg ctcgctcaaa
     8401 ctccatagtt tattctcgac ctcttttttc ccaggccagc cgcttacagc atagcagcgc
     8461 gccagcacgc gtttgacgtt accgtcgaga atcggaaagt gcttacccag agaaagcgag
     8521 agaatcgcgc ctgcggtgga acgcccgacg cccggtaacg ccgcgacttc ttcaaaggtt
     8581 tccgggaatt taccaccgtg taaggtcgcc acttgttgtg ccgctttatg caggttgcgc
     8641 gcgcgggcgt aatagccaag cccggtccac aagtggagaa cttcgtcgag cggcgcattg
     8701 gcgagatcgg tcaccgtcgg gaagtgcgcc ataaagcgtt caaaataggg gataacggtc
     8761 gcaacctgag tttgttgcaa catcacttct gagagccata ctttgtaggg cgtcttgtca
     8821 atttgccagg gcagcgtttt ccgcccgtat ttatcgtacc agtccagaac ctgggctgaa
     8881 aattgcgacg cttgcatggt caccgacttc actgttgttg ggggcaagat tgcagcacag
     8941 cgacggcagg gtgtaaaccg gaactttccg cagccacggc cttatcatgc ttgcatccgg
     9001 caactaactt tggataatgc ccgttttcag aacactttca caagcgacta aatctttatg
     9061 aaaaacgacg tcatttcacc ggaatttgat gaaaacggcc gcccactgcg ccgtatccgt
     9121 agttttgtgc gccgccaggg gcgactgacc aaaggccagg aacatgcgct ggaaaactac
     9181 tggccggtga tgggcgttga gttcagtgaa gatatgctgg atttccccgc gctttttggc
     9241 cgtgaagcgc cggtgacgct tgagattggt tttggcatgg gggcgtcgct ggtggcaatg
     9301 gctaaagatc gccccgagca ggacttcctc ggcattgaag tgcattcacc gggcgttggt
     9361 gcgtgcctgg cttctgcgca tgaagaaggt ttaagcaacc tgcgcgtgat gtgtcacgat
     9421 gcggttgaag tgctgcataa aatgattcct gacaattcat tgcgcatggt gcagctcttt
     9481 ttccctgacc cgtggcacaa agcgcgccat aataaacgcc gtatcgttca ggtgccgttt
     9541 gccgaactgg taaaaagcaa actgcagctg gggggcgtat tccatatggc gaccgactgg
     9601 gaaccttatg cggaacatat gcttgaagtg atgtcttcta ttgacggtta taaaaacctg
     9661 tcagagagca atgattacgt accgcgtccg gcatcacgtc cggtgacgaa atttgaacaa
     9721 cgtggtcatc gtcttggtca cggagtatgg gacttaatgt tcgagagggt gaaataatgg
     9781 caaagaaccg tagccgtcgt ctgcgtaaaa aaatgcacat cgacgaattc caggaattag
     9841 gattttcggt ggcatggcga ttcccggaag gtacatcgga agaacagatt gataaaatcg
     9901 ttgatgactt tattaacgag gttatcgaac cgaacaaact ggcctttgac ggcagcggtt
     9961 atctggcctg ggaaggtctg atctgcatgc aggaaatcgg caaatgcacc gaagaacatc
    10021 aggcgattgt gcgtaagtgg ctggaagagt gcaaactgga tgaggtacgc accagcgaac
    10081 ttttcgacgt ttggtgggac taagaaagca tacgggcgat gacaaatgca aaactgcctg
    10141 atgcgctacg cttatcaggc ctacagagat gcacgatcga gtaggcggga taaggcgttt
    10201 acgccgcatc cggcatggaa aacgcgtact ttgttatcaa tctggggcca gcatatgctg
    10261 gcctgatttg ttcttgaggg aagactatga tgcgcaaaat gctgctggcg gcagcacttt
    10321 cagtgacggc aatgaccgct cacgccgact accagtgcag cgtcacgccg cgtgacgatg
    10381 tgattgtcag cccgcaaacc gtgcaggtga agggcgaaaa cggcaatctg gtgatcacgc
    10441 cagacggcaa cgtgatgtat aacggtaagc aatattccct gaatgccgcc cagcgcgagc
    10501 aggcgaagga ttatcaggct gaactacgta gcacgctgcc gtggattgat gaaggcgcga
    10561 aaagccgcgt cgagaaagcc cgtattgcgc tggataaaat tatcgttcag gagatgggcg
    10621 acagcagcaa aatgcgcagc cgtctgacca aacttgatgc gcagcttaaa gagcagatga
    10681 atcgcattat cgaaacgcgc agcgatggcc tgacgttcca ctataaagcc attgatcagg
    10741 ttcgcgccga aggccagcaa ctagtgaatc aggcaatggg cggaatttta caggacagca
    10801 ttaatgaaat gggcgcgaaa gcggtgctga aaagcggcgg taacccatta cagaatgtgc
    10861 tgggaagctt gggcggtctg caatcctcaa tccaaaccga gtggaaaaag caggaaaaag
    10921 atttccagca gtttggcaaa gatgtttgta gccgcgttgt gactctggaa gatagccgca
    10981 aagccctggt cgggaattta aaataatcct ctattttaag acggcataat acttttttat
    11041 gccgtttaat tcttcgtttt gttacctgcc tctaactttg tagatctcca aaatatattc
    11101 acgttgtaaa ttgtttaacg tcaaatttcc catgcaaagc taagggataa tgcgtagcgt
    11161 ccacgtaact ggaggaatga aatggagttt ttcaaaaaga cggcacttgc cgcactggtt
    11221 atgggtttta gtggtgcagc attggcatta cccaatatca ccattttagc aaccggcggg
    11281 accattgccg gtggtggtga ctccgcaacc aaatctaact acacagcggg taaagttggc
    11341 gtagaaaatc tggttaatgc ggtgccgcaa ctaaaagaca ttgcgaacgt taaaggcgag
    11401 caggtagtga atatcggctc ccaggacatg aacgataatg tctggctgac actggcgaaa
    11461 aaaattaacg ccgactgcga taaaaccgac ggcttcgtca ttacccacgg taccgacacg
    11521 atggaagaaa ctgcttactt cctcgacctg acggtgaaat gcgacaaacc ggtggtgatg
    11581 gtcggcgcaa tgcgcccgtc cacgtccatg agcgcagacg gtccattcaa cctgtataac
    11641 gcggtagtga ccgcagctga taaagcctcc gctaatcgtg gcgtgctggt ggtgatgaac
    11701 gacaccgtac tggacggtcg cgatgtcacc aaaaccaaca ccaccgacgt agcgaccttc
    11761 aagtctgtta actacggtcc tctgggatac attcacaacg gtaagattga ttaccaacgt
    11821 accccggcac gtaagcacac cagcgatacg ccattcgatg tctctaagct gaatgagctg
    11881 ccgaaagtcg gcatcgttta taactacgct aacgcatccg atcttccggc taaagcactg
    11941 gtagatgcgg gctatgatgg catcgttagc gctggtgtgg gtaatggtaa cctgtataaa
    12001 tccgtgttcg acaccctggc aaccgccgcg aaaaacggca ctgcagtagt gcgttcttcc
    12061 cgcgtaccga cgggtgctac cactcaggat gctgaagtgg atgatgcgaa atacggcttc
    12121 gtcgcctctg gcacgctgaa cccgcaaaaa gcgcgcgtcc tgctgcagct ggctctgacg
    12181 caaaccaaag atccgcagca gatccagcag attttcaatc agtactaatc acctcgtccc
    12241 ggcatcgtgc cggggctttt tcacttcaga cttacgtcca ttgccaattt tcattaccct
    12301 aatgataatc accggaataa attattccgc gcgagggttt tcgggtgaaa aagcaatgga
    12361 ttgttggcac ggcgctgctt atgttgatga ctggtaatgc ctgggcagat ggcgaaccgc
    12421 caactgaaaa tatcttaaaa gatcaattca aaaagcagta tcacggcatt ctcaagcttg
    12481 atgccatcag cttaaaaaat cttgatgcta agggtaatca ggccacctgg tcagcggaag
    12541 gcgatgtctc ttccagtgac gatctctata cctgggtcgg tcagttggca gattacgaac
    12601 tgctcgaaca gacctggacg aaagataaac cggtaaaatt ctcggcgatg ttaaccagta
    12661 aaggaacgcc cgcgtctggc tggtcggtga acttttactc ttttcaggcg gcagccagcg
    12721 atcgtgggcg ggtggtcgac gatatcaaaa cgaataataa atatctgatc gtgaatagcg
    12781 aagatttcaa ttatcgcttt agtcagcttg aatctgcgtt gaatacccag aagaactcga
    12841 ttcctgcgct ggaaaaggaa gtgaaagcgc ttgataagca aatggtggcg gcgcagaaag
    12901 cagcggatgc atattgggga aaagatgcaa acggtaagca aatgacccgc gaagaagcct
    12961 ttaaaaaaat tcaccaacag cgtgatgagt ttaataaaca gaacgatagc gaggcgttcg
    13021 cggttaaata tgacaaagag gtttatcaac cggcgattgc ggcatgtcat aaacagagtg
    13081 aagagtgtta tgaagtgccg attcagcaga aacgagattt cgatatcaac gaacaacggc
    13141 gacagacttt tctgcaatca caaaaactca gccgtaaatt gcaggatgac tgggtaacgc
    13201 tcgaaaaagg gcaatatccg ttaacgatga aagtttcaga aataaacagt aaaaaagtcg
    13261 cgattttgat gaaaattgac gatatcaacc aagctaatga acgctagaaa aaagataccg
    13321 aacaactgcg gcgcaatggg gtgattaagt aactcatgtt aagtgccttt gccggataat
    13381 ataaaaagtt atccggcaaa ttatttttta cattagccct agtagctgtg gaatgaacaa
    13441 ggatatttgc ggaatgtaag tgatcatcat caacgctact agcaatgcag cataaaatag
    13501 cagcaatggt ttgattaaat gttgaatctt cacacccgag atagaacaac caacaaatag
    13561 cgcactaccc accggcggtg ttaacagccc aatacaaagg ttggccacca tcataatacc
    13621 gaaatgtact gggtccattc ccagctcttg tgcaatcggc aagaagatcg gtgtaaagat
    13681 tagtacagcc ggtgtcatgt ccataaagat gccgacaatt aataagacga tattaatcag
    13741 cagcaatatg atcagtgggt tatcagaaat ccccatcaat gcgtcgctga tcatataagg
    13801 aatatcagca ttagtcattg cccaggacat gcccacggaa aaaccgatca acagtaatac
    13861 aatcgaagtg gtaacaaccg actccagaat cagtttgggt aaatggcgcc attttacttc
    13921 ccggtaaatc agcaccgaga gaataaatgt gtagacaacg gcaattgctg acgcttcagt
    13981 tgcggtaaaa atgccgccca gaatgccgcc catcacaatg aacaccagaa gcaaacttgg
    14041 taaggcatcc agaaacgctt tacaggcctg agcaaaggtt gctctttctg aaagcgggta
    14101 gccacggcgt ttagcaataa tgccgcagac tatcattacc gcaagaccca ttaagatccc
    14161 tggcaaatat cccgccataa acagcgatgc tacagaaacg ccgccagctg tcagggaaaa
    14221 cacaattaac acgtttgagg gcggtatcag taatccagta atacaagatg agacgtttac
    14281 agcggtagaa aatgccggat cataaccttc tttggtctga atgggattca gtgtgccacc
    14341 aacggcagca gctgccgcaa cggctgaacc agaaatggaa ccaaacatca tattcgccag
    14401 gacgttcaca tgaccaagcg aaccaggaac gcggccaacc attacctggg cgaggttaat
    14461 caaacgtatc gcaataccgc cgctgttcat cagtgttccg gcaaagatga aaaaggggat
    14521 tgccaacagg gcaaagttat ccagtccatt agccagtcgt tgcgaaacag tgattgccgc
    14581 gatatcccag ggaaacatca aaagcattga agctacagtg gcaataccga ttgagaacga
    14641 aatgggcaca ccaataaaga ccagcacaaa aaaacagcca aacagtgtaa tcgcgatata
    14701 actatc
//
LOCUS       DAEBXN010000100        14483 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.100, whole genome shotgun sequence.
ACCESSION   DAEBXN010000100 DAEBXN010000000
VERSION     DAEBXN010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14483
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.100"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(117..383)
                     /locus_tag="KJL99_003195"
     CDS             complement(117..383)
                     /locus_tag="KJL99_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HBD4437821.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPSLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            657..4181
                     /gene="nifJ"
                     /locus_tag="KJL99_003196"
     CDS             657..4181
                     /gene="nifJ"
                     /locus_tag="KJL99_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HBD4437822.1"
                     /translation="MITIDGNGAVASVTFRISEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPSG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRQEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACPGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAANLQKRYDFLAQMAGKAEKSNTD"
     gene            4548..5677
                     /gene="ompN"
                     /locus_tag="KJL99_003197"
                     /pseudo
     CDS             4548..5677
                     /gene="ompN"
                     /locus_tag="KJL99_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            5818..6252
                     /gene="uspF"
                     /locus_tag="KJL99_003198"
     CDS             5818..6252
                     /gene="uspF"
                     /locus_tag="KJL99_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HBD4437823.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            6428..7363
                     /gene="ttcA"
                     /locus_tag="KJL99_003199"
     CDS             6428..7363
                     /gene="ttcA"
                     /locus_tag="KJL99_003199"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HBD4437824.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(7492..8859)
                     /gene="dbpA"
                     /locus_tag="KJL99_003200"
     CDS             complement(7492..8859)
                     /gene="dbpA"
                     /locus_tag="KJL99_003200"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HBD4437825.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVEQSALSLHGDLE
                     QRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTARAG
                     NSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKKAK
                     MRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKTCR
                     VRLLK"
     gene            complement(9337..10320)
                     /gene="zntB"
                     /locus_tag="KJL99_003201"
     CDS             complement(9337..10320)
                     /gene="zntB"
                     /locus_tag="KJL99_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HBD4437826.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRFGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            10575..11807
                     /gene="dgcM"
                     /locus_tag="KJL99_003202"
                     /pseudo
     CDS             10575..11807
                     /gene="dgcM"
                     /locus_tag="KJL99_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
     gene            complement(11828..12391)
                     /gene="smrA"
                     /locus_tag="KJL99_003203"
                     /pseudo
     CDS             complement(11828..12391)
                     /gene="smrA"
                     /locus_tag="KJL99_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
     gene            complement(12645..14099)
                     /locus_tag="KJL99_003204"
     CDS             complement(12645..14099)
                     /locus_tag="KJL99_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HBD4437827.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILF
                     RALQGAAGASMIPLVFTMAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWLTDNLD
                     WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEG
                     ARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFSFSGG
                     VGIFSTVYLIPVFLGQIRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRWLLMA
                     GLGGFVFSMYLFTPITHEWGWQELLFPQAIRGISQQFAMAPIVTLTLGGIPKERLKLA
                     SGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALFFNR
                     SRSDQTSEILASTKLLSQLMLREAQTMAFSVPFC"
     gene            complement(14096..>14483)
                     /locus_tag="KJL99_003205"
     CDS             complement(14096..>14483)
                     /locus_tag="KJL99_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBD4437828.1"
                     /translation="APIDGIVANRSAHTGSWVEGGTSLVSLVPVSELWVDANYKENQI
                     AGMKPGMKAEIRADILKGEVFHGHIESLSPATGASFSLIPIENATGNFTKIVQRVPVR
                     IAFDDAKELKQLLRPGLSVTVSVDER"
BASE COUNT         3862 a         3651 c         3419 g         3551 t
ORIGIN      
        1 gataacacca ttagcgaaat gctgaaagaa ggtgcacaag tggatctgac cgcgaaccag
       61 ttaacgctgg cgaccgcgaa acagacatta acttataagc tggcggattt aatgaattaa
      121 tagctgccac agctcccggc ggcaagtgac tgttcgctac agcgtttgcc gttgggtaat
      181 gcacacatcc caatcgccgt accatccagt tgacgggcaa cagaaagcga accgccgatc
      241 attgcacaat ttgcttctcc actactggac atcgacgctt ttaaacttgg cgctacgtgc
      301 gccgcagtcg cctgctgaac aggctcacta ctacacgccg acaacaataa agcggcacac
      361 cctacccaaa acgctgctcg catctcttct tcctctgatc ttcaagccaa acgacaccgc
      421 cataaataat aggcagcaca gagggcggcg tcgagagctg tcctgcgcgt tgccccgcat
      481 ttttactttt ttatggctat ttttttgccc tctgtttgat caaaacattc attactctga
      541 tgtgggggac acaaaagcga aaatgcagaa gaaagccatt tgctaaaatt gaaggattac
      601 tactgggcgc gcagcaattt cgtgcgcccc tcatttgcgc aatgtaaggg tgtcatatga
      661 ttactattga cggtaatggc gcggttgctt cggtgacgtt tcgcatcagt gaagttatcg
      721 ccatctaccc tattaccccc agttccacga tggcagaaca ggctgatgcc tgggccggaa
      781 acggcttgaa gaacgtttgg ggagacacac cacgcgtggt tgaaatgcag tcggaagcgg
      841 gtgctatcgc taccgtgcat ggcgctttgc agacgggtgc cctttcaaca tcgtttacgt
      901 catcgcaggg tttgctgctg atgatcccga cgctgtacaa actggcaggc gaactgacgc
      961 catttgtcct gcatgtagcg gcacgtaccg ttgccacaca tgcactttct atttttggcg
     1021 atcattccga cgttatggcg gtgcgccaga cgggttgcgc gatgttgtgt gcggctaacg
     1081 tccaggaagc gcaagacttt gctctcattt cgcacatcgc aacgctgaag agccgcgtgc
     1141 catttattca tttctttgat ggttttcgca cgtcccacga aatcaataaa attgtcccgc
     1201 tggccgatga cactattctt gatctcatgc cgcaggccga aattgatgct catcgcgccc
     1261 gggcgctcaa cccggaacat ccggtgatcc gcggtacgtc cgccaatcct gacacttatt
     1321 tccagtctcg cgaagccacc aatccctggt acaacgcggt ctatgaccat gttgaacagg
     1381 cgatgaatga tttcgctgcc gcgacaggtc gtcagtatca gccgtttgag tattacgggc
     1441 atccgcaagc ggaacgggtg attatcctga tgggctctgc cattggcacc tgtgaagaag
     1501 tggttgatga attgctaacc cgtggcgaaa aagttggcgt gctgaaagtt cgtctgtacc
     1561 gtcccttctc cgccaaacac ttgctgcaag ctctgccggg atccgtacgc agcgtggcgg
     1621 tactggacag aaccaaagaa cccggtgccc aggcagaacc gctctatctg gatgtgatga
     1681 ccgcactggc agaagccttt aataatggtg agcgcgaaac cctgccccgt gtcattggtg
     1741 ggcgctatgg tctttcatcc aaagaatttg gcccggactg tgtactggcg gtatttgccg
     1801 agctcaacgc ggctaaacct aaagcacgct ttacggttgg tatttacgat gatgtgacca
     1861 atctgtcact gccgttgccg gaaaacactc tgccaaactc ggcgaaactt gaagccttgt
     1921 tttatggcct tggtagtgat ggcagcgttt ccgcaaccaa aaacaatatc aagattatcg
     1981 gtaattccac gccgtggtac gcacagggct attttgttta cgactccaaa aaagcgggcg
     2041 gcctgacggt ttctcacctt cgcgtgagcg aacagccgat tcgttccgct tatctcattt
     2101 cccaggctga ttttgttggc tgccaccagt tgcagtttat cgataaatat cagatggctg
     2161 agcgtttaaa acctagcggc attttcctgc ttaatacgcc gtacagcgca gatgaagtgt
     2221 ggtcgcgctt gccgcaggaa gttcaggccg tgttaaacca gaaaaaagcg cgcttctatg
     2281 tgattaacgc ggcgaaaatc gcccgcgaat gtggcctggc ggcccgtatt aataccgtca
     2341 tgcagatggc ttttttccat ctgacgcaaa ttctgcctgg cgatagcgcc ctcgcagaat
     2401 tgcagggtgc gattgccaaa agttacagta gcaaaggtca ggatctggtg gagcgcaact
     2461 ggcaggctct ggcgctggcg cgtgaatccg tagaagaagt tccgttgcaa ccggtaaatc
     2521 cgcacagcgc caatcgaccg ccagtggttt ccgatgccgc ccctgatttc gtgaaaaccg
     2581 taaccgcggc gatgctcgcc gggcttggtg atgccctccc cgtttcggcg ctgccgccag
     2641 acggcacctg gccgatgggc accactcgct gggaaaaacg caatatcgcc gaagagatcc
     2701 ccatctggaa agaggaactc tgtacccaat gtaaccactg cgtcgccgct tgcccacact
     2761 cagctattcg cgcaaaagtg gtaccgcctg aagcgatgga aaacgcccct gccagcctgc
     2821 attcgctaga tgtgaaatcg cgtgatatgc gcgggcagaa atatgtcttg caggtggcac
     2881 cggaagattg caccggttgt aacctgtgcg tcgaagtttg cccggcgaaa gaccgtcaga
     2941 atccagagat taaagccatc aatatgatgt ctcgccagga acatgtcgaa gaagagaaaa
     3001 tcaattacga tttcttcctc aacctgccag aaatcgaccg tagcaaactg gaacgtattg
     3061 atattcgtac atcgcagctg attacaccgc tgtttgaata ttcaggtgct tgccccggtt
     3121 gtggcgagac gccgtatatt aaattgctga ctcagctcta tggtgaccgg atgttgatcg
     3181 ctaacgccac cggctgttct tcaatttatg gcggtaacct gccctctaca ccgtatacca
     3241 ccgatgccaa cggtcgtggg ccggcatggg cgaactctct atttgaagat aatgccgaat
     3301 ttggccttgg tttccgcctg acggtcgatc aacaccgtgt ccgcgtgctg cgtctgctgg
     3361 atcaatttgc cgataaaatc ccggcggaat tactgacggc gttgaaatca gacgctacgc
     3421 cagaggttcg tcgagaacag gtcgcagctt tacgccagca actcaacgat gttgccgaag
     3481 cacatgaact tctgcgtgac gcagatgcac tggtggaaaa atcaatctgg ctgattggtg
     3541 gcgacggctg ggcttacgat atcggctttg gcggtctgga tcatgtattg agtttgacgg
     3601 aaaacgtcaa cattctggtg ctggatacgc aatgctattc caacaccggt ggtcaggcgt
     3661 cgaaagcgac accgctgggt gcagtaacta agttcggcga gcacggcaaa cgtaaagcgc
     3721 gtaaagatct tggcgtcagt atgatgatgt acggtcatgt ttatgtggcg cagatttctc
     3781 tcggcgcgca gctgaaccag acggtgaaag cgattcagga agcggaagcg tatccgggac
     3841 catcgctgat cattgcttat agcccgtgtg aagagcatgg ttacgatctg gctctcagcc
     3901 acgaccagat gcgccaactc actgctaccg gcttctggcc gctatatcgc tttgatccgc
     3961 gtcgtgccga tgaaggcaaa ctgccgctgg cgctggattc acgcccgccg tcagaagcac
     4021 tggaagaaac gttacttcac gagcagcgtt tccgtcgtct gaattcgcag cagccagaag
     4081 tggcagaaca gttatggaaa gatgctgcag ctaatttgca aaaacgctat gacttcctag
     4141 cacaaatggc cggaaaagcg gaaaaaagca acaccgatta aatgctctgg ataaggatta
     4201 tccaattcta aaaaaaaagc ccggacgact gttcgggctt gtctttttat atgttggaaa
     4261 atcagtggca atgcaatggc ccaacagaaa ataaaatctg tgacaataaa ggcatataac
     4321 ccgcgcagaa taacgtatag gaaattaaaa gtataatttt tattttgtat gtattatctt
     4381 cgatacattc accagactta ttatcatttc agaggaatta tttgattaag gttttactta
     4441 aggcgtaaca aatgatattt atcggctaac tgaacttctc ctttaggatg ttttcactcc
     4501 ccgaaatggg gataaaaggc aaataaaata actaaaggat ttattcaatg aaaagaaaag
     4561 tactggcact tttaattcct gccctgctcg ccgcaggtgc tgcacatgca gccgaagttt
     4621 ataataaaga cggcaacaaa ttagatctgt atggcaaagt tgatggcctg cattattttt
     4681 ctgataattc agcgaaagat ggcgaccaga gctatgcgcg tctgggtttt aaaggcgaaa
     4741 cccaaattaa cgatcaactt actggctacg gtcaatggga atacaatatt caggcaaaca
     4801 acactgaatc ttcaaaaaac cagtcatgga cccgtctggc atttgccgga ctgaaatttg
     4861 cagattacgg ttctttcgat tacggacgta attatggcgt aatgtacgac atcgaaggct
     4921 ggaccgatat gctgcctgaa tttggcggtg actcttatac caatgcagac aactttatga
     4981 ctggtcgagc caatggcgtc gcgacttatc gtaatactga tttcttcggt ctggtaaatg
     5041 gtctgaactt cgcggtgcag tatcaaggta acaacgaagg agccagtaat ggtcaggaag
     5101 gcaccaataa cgaacgcgat gttcgtcatg aaaacggtga cggctggggt ctttccacaa
     5161 catatgattt aggcatgggc tttagcgctg gtgcggcata cacctcttct gaccgcacca
     5221 atgaccaggt taaccatact gcggcgggtg gtgataaagc agacgcgtgg actgctgggc
     5281 taaaatacga tgctaacaat atttacctag caaccatgta ttcagaaacg cgtaatatga
     5341 ccccgtttgg cgacagcgat tatgcagttg cgaacaaaac ccagaacttt gaagttacta
     5401 gtaccagttt gatttttgtc tgcgtccggc agtctctttc ctgatgtcta aaggccgtga
     5461 ccttcacgct gctggtggtg cagacaaccc ggcaggtgtt gatgataaag atctggttaa
     5521 atacgccgat gttggcgcga cttactattt caataaaaac atgtccacct atgttgacta
     5581 taaaatcaac ctgttggatg aagatgacag tttctacgct gccaacggca tctctaccga
     5641 tgatattgtc gctttaggtc tggtttatca gttctaaatc ctcctgcccg ctgttatggc
     5701 gggctttttc tgcttattct tcctcctttg atctaaatta aaaatgtgaa ctccgtcatt
     5761 acacaaaaag tgtcatctgg cgttacactt tatgcggata ctaaaacagg aggttttatg
     5821 aacagaacga ttcttgtccc tatcgatatt tcagattcag aattaactca acgcgtgatt
     5881 agccacgttg aggcagaggc aaagattgat gatgcagagg ttcatttcct gacggtaata
     5941 ccttcactgc cctactatgc ctctctgggt ttagcgtatt ccgcagaatt accggcaatg
     6001 gatgacctga aagcggaagc caaatcgcaa ctggaagaga tcattaaaaa atttaaactg
     6061 ccaaccgaca gagtgcatgt ccatgttgag gaaggctcgc ccaaagaccg cattctggaa
     6121 ttggcgaaga agatccccgc tcatatgatc atcattgctt cccatcgacc ggatatcacc
     6181 acttatctgc tcggttccaa cgccgcagct gtagtgcgtc acgcagagtg ctccgtgctg
     6241 gttgtgcgct gacactaacg cccgcacatt gctgcgggct ttttgattca tttcgcaaat
     6301 gtgctgacat tttcccctct aatccgtacc atacacgcca cagtttttat atcagatttc
     6361 ttatgctgag cgttccggca tgacgcatac tcttctgatg ccatataacg aattgagtcg
     6421 cttttaaatg tcgcaaaatc aagaaattag taagaaagaa caatacaacc tgaacaaatt
     6481 acaaaaacgt ctgcgtcgta acgtgggcga agccattgct gacttcaata tgattgaaga
     6541 aggcgatcgc atcatggttt gcctctccgg gggtaaagac agctatacca tgctggagat
     6601 tctgcgcaat ttgcagcaaa gcgcgccaat caatttttcg ctggtggctg ttaacctcga
     6661 tcaaaagcaa ccgggcttcc cggaacacgt tctgcccgag tatcttgaaa agctgggcgt
     6721 tgagtacaag attgttgaag agaacactta cggtatcgtg aaagagaaga ttccagaggg
     6781 caaaaccact tgctcactgt gttctcggct acgtcgcggt atcctttatc gcaccgcaac
     6841 ggaactgggg acgacgaaga tcgcgttggg tcaccatcgt gacgatatcc tgcaaacgtt
     6901 gttcttaaat atgttctacg gcggcaagat gaaaggtatg cctccgaaac tgatgagcga
     6961 tgatggcaaa catatcgtta ttcgtccact ggcctactgc cgcgagaaag atattcagcg
     7021 atttgccgat gcaaaagcgt tcccgattat tccgtgcaac ctgtgcggtt cacagcctaa
     7081 cctgcaacgt caggtgattg ctgacatgtt gcgtgactgg gataaacgtt atccagggcg
     7141 tatcgagacg atgttcagcg cgatgcagaa tgtggtgccg tcgcatctgt gcgataccaa
     7201 cctgttcgat ttcaaaggca ttacccacgg ttctgaagtg gttaacgggg gtgatctggc
     7261 gtttgatcgc gaagagatcc cactacaacc ggcgggctgg cagccagaag aagatgaaaa
     7321 tcagttggat gagttacggc tgaatgtggt tgaagtgaaa taaccaggat agcgcccgat
     7381 gcgcaagcgt atcgggctac tcttatggag gccggataag acgcggccag cgtcgcatct
     7441 ggcaatcccg aataagatgt ttactcttgc acccggcaat tcaacatttc attattttaa
     7501 taaccgcacc cggcacgttt ttcctttaat cttcccgccc tgtaactgtt tccatgcttt
     7561 atgagcaaca gcctgacgga ccgcgacata gacatgcgcc ggatgcacgg cgattttgcc
     7621 aatatctgcg ccatcaagcc cgatatctcc tgtcagtgca cctaatacat cacccgggcg
     7681 cattttggct tttttcccgc catcgataca caacgttgcc atttctgctt ccagcgtcac
     7741 aatggaacta ttagctggcg gcgtttgcca gttaagtttt atctgcaaca tgtcagaaat
     7801 gatattggcc cgctgtgctt cttccggagc acagaaactg atcgccagac cgctatttcc
     7861 tgcacgagct gtacgaccga tgcgatgtac atgaacttca gggtcccacg ccagctcaaa
     7921 gttcaccacc agctcaagcg atttaatatc cagaccacgc gcagcaacat cagtcgcaac
     7981 cagtacacgg gcgctaccgt tagcaaaacg taccagggtc tgatcgcgtt gctccagatc
     8041 gccgtgtaac gacaatgcac tttgctctac ttcattcagc gcgtcgcaga cagcctggca
     8101 atcttttttg gtattgcaaa acaccacaca agaggatggc tgatgcaagc ttaataaccg
     8161 ttgcaacaga ggaattttgc ctttactgga tgtctcataa aattgttgtt caatgggtgg
     8221 caaagcatct gttgagtcaa tttcaatcgc caaaggatcg cgttgcactc gtccgctgat
     8281 tgcggcgatg gcttccggcc aggttgccga aaacagaagc gtctgtcgag atgcaggcgc
     8341 aaaacggatg acatcatcaa tggcatcgct aaatcccata tccagcatgc ggtcggcctc
     8401 atccatcacc aaagtattca gcgcatccag tgataccgtg cctttttgca agtgatccag
     8461 caaacgaccc ggcgttgcca cgataatatg cggcgcatgt tgcagcgaat cacgctgcat
     8521 accgaacggt tgaccaccgc acaacgtcaa aattttggta tttggcagaa aacgcgccag
     8581 ccgacgcaat tcacctgcca cctgatccgc cagttcacgc gtaggacaca gcactaaagc
     8641 ctgggtttga aatagcgacg catcaatttg ctgtaacaag ccgaggccaa aagccgccgt
     8701 tttgccgctg ccggttttcg cctgcacgcg aacatctttt ccggcaagga tcgccggaag
     8761 cgcggcggcc tgcaccggcg tcatggttaa ataacccaac tcattaaggt tcgtgagttg
     8821 ggcgggaggc aaaacattca gggtagaaaa agcggtcaca atctattctc gtggtcatcg
     8881 acgcaaagtt agcaggcgcg tatcctcgca gatctacgct cacgatgcga caatttaatc
     8941 ggttcttcat cgggtggtgg gtcaggcatg ggttgcgggc gagggatcgg atcgggcact
     9001 ggaacaggat cgccaggaat cggttcaggg acaggaattt gcaaataaat aagtgtcgtc
     9061 atatttccct ccggtcattg ggtggactct taaagggtag acgctgataa ataacaggca
     9121 aaaaaaagcc gactcatcaa agtcggcgtc gtacgaatca attgtgctat gcagtaattc
     9181 aaaaaaggaa gtaagacaat atggagcgca acgcccatcg cttgacgttg cattcacctg
     9241 caagggagat attgccctga atgggtagag agtttattga cttcgctcaa actttgcggc
     9301 gtttttgtat acagacagcc ggaaaaattg cttttgttac aaccatttac tacggtgcaa
     9361 ccataaagca acaccaccaa taagaacaac taacagaata caaaaaattg aaaatccgaa
     9421 ttgccacccg ccgccaggga tcccaccaag gttgacgcca aataacccgg tcagaaaggt
     9481 actgggtaaa aagaccattg ccatcaacga cattgtatag gtacgacgag ctaaattttc
     9541 ctgcatcacc tgagcgattt catccgccat cacgccagtc cgtgctatac aggcgtcgat
     9601 ttcgtcaagg ccgcgcccaa ggcgatcggc aatatcctgc atccgacggc gttggtcatc
     9661 gctcatccac ggcaaacgtt cactggcaag acgagcataa acatcacgtt gcggtgccat
     9721 atagcgacgc atcacaatta attgtttgcg cagcagagcc aggaatccac gcggtggaat
     9781 ttgctgatca aggagattat cttcaaggtc gataatttta tcgtgcagct gctcgataaa
     9841 ttcactggaa tgatcggtca acgcatcgca cacatccacc agccatcccc cgcaatcggt
     9901 cggacccgtg ccctcttcca gatcgctcac cacatcgtcc agcgccagca ctttgcgttg
     9961 tcgggtcgaa acaattaacc gcccgtccat atatacacgc atggcgacca gttgatcggg
    10021 gcgttcatcg gtgctgccgt ttatacagcg caatgtaatc agcgtgcctt caccgaaacg
    10081 gctgactcgg ggccgtgtgc tctcgcccgc cagcgcatca cgtacgttat tgggaagcag
    10141 cggtgttgtc gccagccatt gggcgctatc atggtgtaca taattaaggt ggagccagca
    10201 gggatgcgct tcatcaatca catctgtatt ttccagcggt ttaacgccgc ctctaccatc
    10261 cagcatccag gcaaatactg catccgggac attaacgtcc gatcccttaa tcgcttccac
    10321 tgtgcctcca tcatcaacgc attattttgt agtctagcct tctggccctg ttacgcaaca
    10381 tctcatcacc ccattaccct gaaatgatta ataaaattct gtctaaattg aatacaaaaa
    10441 gcaaaatgct tttccgtata caaaccgtgt gaagtgttaa atagcgtcta tcattatcag
    10501 aattatctga tcatatgacg tggctttttt gcgatcggat agcaacaaaa attgataaaa
    10561 ataacgggat ctcaatgatt acgcacaact tcaataccct ggacttactc accagtcctg
    10621 tctggattgt ttcgcccttt gaggaacagt taatttatgc caatagcgcg gcgcgactgt
    10681 tgatgcaaga cctcacgttt agtcagctac gaaccggacc ctattccgtc tcctcacaaa
    10741 aagaactgcc gaaatacctc tccgatctgc aaaaccaaca cgatattatc gaaatcctca
    10801 ctgttcagcg taaagaagag gaaacagcat taagctgtcg gcttgttttg cgagagctga
    10861 cagaaacaga accggtgatt attttcgaag gtatcgaagc gccggcaacg ctgggtttaa
    10921 aagccagtcg ctcggcaaat tatcagcgca aaaaacaagg tttttatgcg cgcttttttc
    10981 tgactaactc tgcaccaatg ttgttgattg acccgtcacg agatggacaa atcgtcgatg
    11041 ctaacctcgc cgcgctcaat ttctatggtt ataaccatga aacgatgtgc cagaaacata
    11101 cctaggaaat aaatatgctt gggcgtcgcg tcatgcctat catgcatgaa atttcgcatt
    11161 tacccggcgg tcataaccct ttgaattttg ttcataaact ggcggatgga tctactcgtc
    11221 atgtgcagac ctatgccgga ccgattgaaa tttatggcga caagctcatg ttatgtattg
    11281 tgcatgatat tactgagcaa aaacggctgg aggagcagct ggaacatgct gctcaccatg
    11341 acgcgatgac cggattactg aatcggcgac agttttatca cattacggaa ccaggccaaa
    11401 tgcagcatct cgccatcgct caggattaca gcttgttgct catcgacacc gatcgtttta
    11461 aacacattaa cgatctctat gggcattcta aaggtgatga ggtgttatgc gccctcgccc
    11521 gccccctcga aagttgcgct cgcaaaggcg atttggtgtt tcgttgggga ggcgaagagt
    11581 ttgtcttatt gctaccaaga accccactgg ataccgcgct ttcgctggct gaaactatcc
    11641 gcgtaagcgt ggcaaaagtg agtatttcgg gcttaccacg ctttaccgtc agcattggtg
    11701 tggcgcatca cgaaggaaat gaaagcatcg atgaactgtt taaacgcgtt gatgatgctt
    11761 tgtatcgggc gaaaaatgat ggacgcaacc gcgtgctggc ggcataagcc gcggatgcgt
    11821 ctcgagatca acgactgcgc ttagcgtggc gctcccagtt ttcttgcttc gcctgcgccg
    11881 ttttacgtag tgcgacgtaa cacgccccgc tgccgccatg atgcggtagc gcggtgcaat
    11941 atgcctgaac atcatcaaat tcggtcagcc agcgcgccac atagctgcgg acaatattgg
    12001 catgtgattt atcttcccgc cctttaccat gaataatcag cacattacgc aaaccatccg
    12061 ccagggcttg ttgaatgaaa ctgaacacca ttttgcggca ctcttccacc ggctggcgca
    12121 aaagattcag gctcgcctgt tgcggatatt taccactgcg cagcttatcc agcaccccat
    12181 gctacaaccc ttcctgccga aactccagcg gctgacttag cgggatgatg tcgagaaatc
    12241 cggtagtgag gaaattatca agctgcagcg tgtcgatacg ctgcggcgca cgttggttac
    12301 gcgttggatg ccagtggaca tctgtagcac gtttcagcgg ctggacatct tccatggcgt
    12361 caagaaacag cgatttgtcg tcaaggttca tgtaacctcc tatgcacctt cacacgtcgt
    12421 gattcagacg ggttaattgt tcatacttac tgcatcgttc ccttcacaga ttgaaacaat
    12481 actctacttc atacctgtga gtatctgcgc aggcggggca ttttatacta gcctgttagc
    12541 agtatcgaac agtttagccc gagtttaatt cagcacaggc tccgatcagg atgatttatt
    12601 catagcagga accaacaaaa atgcgataaa taacagacca ctgattagca gaaaggtacc
    12661 gaaaatgcca tagtttgcgc ctccctcagc atcaattgac tcaataattt ggttgaagcc
    12721 agtatttccg atgtctggtc acttctactg cgattaaaaa ataaagcact tctggaaatg
    12781 aagtcattca ccgtatgggg aacagaaacc attttttcac ccattctcga aaaatgaaaa
    12841 ttagttcgat tattgagaat agagccacac agggcaatac caatcgcgcc cccaagatta
    12901 cgtgttaaat taaatacacc agaggctaac ttcagccgtt cttttggaat tccccccagc
    12961 gttaatgtta cgatgggggc catcgcaaac tgctgcgaaa tccctctaat tgcctgtgga
    13021 aacagtaatt cctgccatcc ccattcgtgg gttatagggg taaaaagata cattgaaaac
    13081 acaaatccgc ccagccccgc catcaacagc catcgcagat ttattttttt acttaaccag
    13141 aagtaaaagg gaacagaaaa taactggaaa atacctgttg tgcaaacggc aaaacctatc
    13201 tcttcggcat ttaatccgcg aatctgcccc aaaaacacag gtattaaata tacagtagag
    13261 aatatcccaa cccctccaga aaaagaaaaa aaacatccca gagtgaaatt tttatcctta
    13321 aaagcatgaa gatccattat cggattggat attgtaagtg tacgcgcagc aaataaaata
    13381 aaactcacca atgccagaac tgatgtcagc aaaatagtat tatcatccag ccatccccag
    13441 cgcgctcctt cttcaagggt atattccaga catcccagag tcatggccaa caggataata
    13501 ctcggataat cagcaacttt cagtaaagag agatcaggtt tatcaaaatt aaccagaaaa
    13561 ggaatactca aaactaaata tatgccaggt aatatattga tataaaatag ccatcgccag
    13621 tcgaggttat cagtgagcca gccccccaga gttggcccta gcgtcggtga cagtgaggcc
    13681 agcgcactga ctaccgcagc agccaacccc aactctttac cctggtaata aataaacgcc
    13741 atcgtgaaaa ctaaagggat catggatgcg ccagcagctc cctgtaaagc acgaaacaaa
    13801 atcatgatct gaatattcca tgcaagtcca cacgcgatac tcattaaagt aaatattccc
    13861 gcggatagag taaaaagcca acgagtggaa aatacccgtg acagccaccc agagagcgga
    13921 ataacaataa tctcagcaat taaatagctg gtctgaagcc aggccatttc gtctttacct
    13981 gcggacagac caccacctat ttcatttagg gatgacgata caatttgaat atcaagctga
    14041 gcaataaata atcccataca catactggcg aaagcaatat gtttacgcat agacatcatc
    14101 tctcatcaac actgacggtg acggacagtc ccgggcgaag aagttgcttt aattcctttg
    14161 cgtcgtcaaa tgcaatccgt accgggacac gctgaacaat tttggtgaaa ttgccagtgg
    14221 cattttctat cggaatcaag ctgaaactgg ctcctgtcgc tggggaaagg ctttcaatgt
    14281 gaccgtgaaa cacttcccct ttgagaatat cagcacggat ctcagccttc attcctggct
    14341 tcattcccgc tatttggttt tctttataat ttgcatcaac ccacaactca ctgactggta
    14401 ctagtgaaac taacgatgtg ccgccttcca cccaactgcc tgtatgggcg ctgcggtttg
    14461 cgacaatgcc atcaatcggc gca
//
LOCUS       DAEBXN010000101        14465 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.101, whole genome shotgun sequence.
ACCESSION   DAEBXN010000101 DAEBXN010000000
VERSION     DAEBXN010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14465
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.101"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(14..1990)
                     /gene="speA"
                     /locus_tag="KJL99_003206"
     CDS             complement(14..1990)
                     /gene="speA"
                     /locus_tag="KJL99_003206"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HBD4437829.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPVE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(1999..2130)
                     /gene="yqgB"
                     /locus_tag="KJL99_003207"
     CDS             complement(1999..2130)
                     /gene="yqgB"
                     /locus_tag="KJL99_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HBD4437830.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAATVVNCFTLNKII"
     gene            2266..2481
                     /locus_tag="KJL99_003208"
     CDS             2266..2481
                     /locus_tag="KJL99_003208"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4437831.1"
                     /translation="MGITSAGMQSRDAECGERVFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            2785..3939
                     /gene="metK"
                     /locus_tag="KJL99_003209"
     CDS             2785..3939
                     /gene="metK"
                     /locus_tag="KJL99_003209"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HBD4437832.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            4376..5770
                     /gene="galP"
                     /locus_tag="KJL99_003210"
     CDS             4376..5770
                     /gene="galP"
                     /locus_tag="KJL99_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HBD4437833.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGIGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            5847..6344
                     /locus_tag="KJL99_003211"
     CDS             5847..6344
                     /locus_tag="KJL99_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HBD4437834.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAIYRCVHCGE
                     QLVAK"
     gene            6439..7146
                     /gene="endA"
                     /locus_tag="KJL99_003212"
     CDS             6439..7146
                     /gene="endA"
                     /locus_tag="KJL99_003212"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HBD4437835.1"
                     /translation="MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            7226..7957
                     /gene="rsmE"
                     /locus_tag="KJL99_003213"
     CDS             7226..7957
                     /gene="rsmE"
                     /locus_tag="KJL99_003213"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003853382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HBD4437836.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            7970..8920
                     /gene="gshB"
                     /locus_tag="KJL99_003214"
     CDS             7970..8920
                     /gene="gshB"
                     /locus_tag="KJL99_003214"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HBD4437837.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTCYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            9029..9592
                     /locus_tag="KJL99_003215"
     CDS             9029..9592
                     /locus_tag="KJL99_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HBD4437838.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            9592..10008
                     /gene="ruvX"
                     /locus_tag="KJL99_003216"
     CDS             9592..10008
                     /gene="ruvX"
                     /locus_tag="KJL99_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HBD4437839.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(10184..11164)
                     /locus_tag="KJL99_003217"
     CDS             complement(10184..11164)
                     /locus_tag="KJL99_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HBD4437840.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQKVGMLTFQQSYQQRVGEGRL"
     gene            11182..11886
                     /gene="yggS"
                     /locus_tag="KJL99_003218"
     CDS             11182..11886
                     /gene="yggS"
                     /locus_tag="KJL99_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HBD4437841.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNEQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPALESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            11904..12470
                     /gene="yggT"
                     /locus_tag="KJL99_003219"
     CDS             11904..12470
                     /gene="yggT"
                     /locus_tag="KJL99_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HBD4437842.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQCAHCDFYTPFSQFVVKVA
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFEVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYAINMGVAEVLQATGNMLLPGLWMAL"
     gene            12467..12757
                     /locus_tag="KJL99_003220"
     CDS             12467..12757
                     /locus_tag="KJL99_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HBD4437843.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            12765..13358
                     /locus_tag="KJL99_003221"
     CDS             12765..13358
                     /locus_tag="KJL99_003221"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HBD4437844.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            13351..>14465
                     /gene="hemW"
                     /locus_tag="KJL99_003222"
     CDS             13351..>14465
                     /gene="hemW"
                     /locus_tag="KJL99_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HBD4437845.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLL"
BASE COUNT         3476 a         3834 c         3727 g         3428 t
ORIGIN      
        1 ggaataatct taattattca tcttcaagat aagtataacc gtacaaacct gcctcgaact
       61 cttcaaggaa ctgttgttgc agttcagcat caagatcggt tttcttcact tgatcgcgga
      121 actgggttaa cagcgttttc ggatcgagct gtacatattg cagcatgtcc gccacggtat
      181 cgccttcgtc agacagttct acttctacgc taccgtcagg gaacacgaac acgtcaactg
      241 cttcggtatc accgaacagg ttgtgcatat tgccaaggat ctcctgatat gcaccgacca
      301 taaagaaacc gagcatcggc ggattctctg gatcgtactc cggcattggc attgtcgtgg
      361 caataccatc accatcaata tagtggtcga tagcaccgtc agagtcacag gtaatatcca
      421 gcagcacagc gcgacgttcc ggcacttgat ccagcccttc cagcggtaga accgggaaca
      481 actggtcgat cccccatgcg tccggcattg actggaacag cgagaaattg acgtacattt
      541 tgtccgccat acgttcctgc agttcgtcga ttatcggacg atgcgcacgg ttttgcggat
      601 ccagttgctt ctgcacttca tggcacatgc tcagatagag ttgttctgcc catgcacgtt
      661 cttgcaggct aaaggtgccg gaagagtagc cgatatgaat gtcgtgcaga tccatctggc
      721 tgtcgtgtaa ccattcacgc aaagaacggc gagttcccgg ttcgtgcatt tcctgccagg
      781 tttcccacat gctttgcagc gcacgcggcg catcttctac gggcgcggtc ggcaccgtat
      841 attcgttacg ttccacgccg atgatattcg acaccagcac ggtatggtgc gcagtcaccg
      901 cacggcccga ttcggtgatt accgtcggat gcggcagacc attttcttca cacgcatcgc
      961 caatcgccca gatgatgttg ttggcgtatt cattgaggcc atagtttacg gagcagtcag
     1021 actgcgaacg ggtgccttca taatccacgc ccagaccgcc gccgacgtcg aagcactgaa
     1081 tattgacgcc cagcttgtgc agttccacat agaaacgcgc ggattcacga acgcctgtcg
     1141 cgatatcgcg aatattcgcc atctgcgaac cgaggtggaa gtgcagtagt tgcaggctgt
     1201 cgagacgccc ggcttcacgc agggtttcaa ccagttgcag tacctgagtc gcagccaggc
     1261 cgaacttcga tttttccccg ccggaggact gccatttacc cgagccctgc gaagccagac
     1321 gtgcacgcac gcccagacga ggaacgacat tcagacgttc tgcttcatcc agcacaatgg
     1381 cgatttctga catcttctca atgaccagat agaccttgtg ccccatcttc tcgccaatta
     1441 atgccaggcg gatatattcg cggtctttat aaccgttgca gacgatgacg ctacgagtca
     1501 tgccagcatg tgccagcact gccatcaact cggctttgga accggcttcc agacccagcg
     1561 gttcacccga atgaatcagg gactcgatca cgcggcggtg ctggttaact ttgatcggat
     1621 aaacaaggaa gtaatcgccg ttatagccgt aggattccct cgcacgtttg aacgcggcgt
     1681 taatggaacg caaacggtgc tgcaggatct gtgggaaaca gaacagtgca ggcagacgct
     1741 gaccctgtgc ttcacgagtt ttcactaact gcgcgagatc gacgcgagct tccgggacgt
     1801 ccgggtccgg gcacacgctg atgtggccca gctcgttaac gtcatagtag ttattgcccc
     1861 accaggcaat attgtaagta cgcagcatct tgctggcttc ctgggagctc attgcaacct
     1921 cctgcatgga gcgtagtaca ccgtgttcgc ccgctgacga aggcaaaccc atagacatgt
     1981 cgtcagacat agcgaacctc aaattatttt attaagtgta aaacagttaa cgactgtcgc
     2041 agcctggaac tgcgataaca acccataagc acatggattt tccagcagtg tatgctgacg
     2101 ctccgactgc gcaaccggtt tctttttcat aacattatta agcacataac cgaacgtaag
     2161 tgtgacagtt cggcgaaacc acgagaaaac tcttgttttt acaagagcgc ccttgttcag
     2221 tcctcagtaa ctgtaaccag ctcttgaatc ctgagaagcg ccgagatggg tataacatcg
     2281 gcaggtatgc aaagcagaga tgcagagtgc ggggaacgag tcttcaccag aacggtgaga
     2341 caggttaagc agcagacaac ggttcattat ttcgtatcac ctccacggcc gcctgttaag
     2401 acgaacccac aagccaaaac tctgatttca acccggctgg aagtggcaac acgaaagaaa
     2461 cgtcgtgtgc tttttattta agccgcgcgc cgcgttttat accccacaat ggcagaaaat
     2521 tgcaaaagat aaatacgcag aatgccggca ttgtcaggaa aaatttccag ccacgttttt
     2581 aacagaatga gacacgattc aaaaaaaagt ggaaatcggg tgaagaattg acctaaaata
     2641 gccatccaga tgttaatcca tccataccga ttaacactca gactgccagt gtttttaacc
     2701 tgcagagtcg tggtaggatc cgctaccaca gaaaatccac acaacagttt gagctaacca
     2761 aattctcttt aggtgatatt aaatatggca aaacaccttt ttacgtccga gtccgtctct
     2821 gaagggcatc ctgacaaaat tgctgaccaa atctctgatg ccgttttaga cgcgatcctc
     2881 gaacaggatc cgaaagcacg cgttgcttgc gaaacctacg taaaaaccgg catggtttta
     2941 gttggcggcg aaatcaccac cagcgcctgg gtagacatcg aagagatcac ccgtaacacc
     3001 gttcgcgaaa ttggctatgt gcattccgac atgggctttg acgctaactc ctgtgcggtt
     3061 ctgagcgcta tcggcaaaca gtctcctgac atcaaccaag gcgttgaccg tgccgatccg
     3121 ctggaacagg gcgcgggtga ccagggtctg atgtttggct acgcaactaa tgaaaccgac
     3181 gtgctgatgc cagcacctat cacctatgca caccgtctgg tacagcgtca ggctgaagtg
     3241 cgtaaaaacg gcactctgcc gtggctgcgc ccggacgcga aaagccaggt gactttccag
     3301 tatgacgacg gcaaaatcgt tggtattgat gctgtcgtgc tttccactca gcactctgaa
     3361 gagatcgacc agaaatcgct gcaagaagcg gtaatggaag agatcatcaa gccaattctg
     3421 cccgctgaat ggctgacttc tgccaccaaa ttcttcatca acccgaccgg tcgtttcgtt
     3481 atcggtggcc caatgggtga ctgcggtctg actggccgta aaattatcgt tgatacctac
     3541 ggcggcatgg cgcgtcacgg tggcggtgca ttctctggta aagatccatc aaaagtggac
     3601 cgttccgcag cctacgcagc acgttatgtc gcgaaaaaca tcgttgctgc tggcctggcc
     3661 gatcgttgtg aaattcaggt ttcctacgca atcggcgtgg ctgaaccgac ctccatcatg
     3721 gtagaaactt tcggtactga gaaagtgcct tctgaacaac tgactctgct ggtacgtgag
     3781 ttcttcgacc tgcgcccata cggactgatt cagatgctgg atctgctgca cccgatctac
     3841 aaagaaaccg cagcatacgg tcactttggt cgtgaacatt tcccgtggga aaaaaccgac
     3901 aaagcgcagc tgctgcgcga tgctgccggt ctgaagtaat ctttcttcac ctgcgttcaa
     3961 aggccagcct cgcgctggcc tttttctttt acctcaccgc ggtggatagg cgttcacgcc
     4021 gcatccggca aaaaaactgc ccgcacaata acatcattct tcctgatcac gtttcacggc
     4081 aaattaccat cacaactgaa accgattaca ccaaccacac cagcaaaata tttgtaatat
     4141 tttcatatta ttattcagct ttcacagttg ttacatttct tttcagtaaa gtcttaattg
     4201 cagatgacag cgtttaatct atgatgatat aactcaatta ttttcatgcc cttaaatcat
     4261 aactaagata aatgttagtg taagcgatta cactgatgtg atttgcttca catcttttta
     4321 cgtcgtactc acctatctta attcacaata aaaaataact atattggagg gcatcatgcc
     4381 tgacgctaaa aaacaggggc ggtcaaacaa ggcaatgacg tttttcgtct gcttccttgc
     4441 cgctctggcg ggattactct ttggcctgga tatcggtgta attgctggcg cactgccgtt
     4501 tattgcagat gaattccaga taacttcgca cacgcaagaa tgggtcgtaa gctccatgat
     4561 gttcggtgcg gcagtcggtg cggtgggcat cggctggctc tcctttaaac tcgggcgcaa
     4621 aaagagcctg atgatcggcg caatcctgtt tgttgccggt tcactgttct ctgcggctgc
     4681 gccaaacgtt gaagtactga ttctttcgcg cgttctgctg gggctggcgg tgggtgtggc
     4741 ctcttatacc gcaccactgt acctgtcaga aattgcgccg gaaaaaattc gcggcagtat
     4801 gatctcgatg tatcagttga tgatcactat cgggatcctc ggtgcttatc tttctgatac
     4861 cgccttcagc tacaccggtg catggcgctg gatgctgggt gtgattatca tcccggcaat
     4921 tttgctgctg attggtgtct tcttcctgcc agacagccca cgttggtttg ccgccaaacg
     4981 ccgttttgtt gatgccgaac gcgtgctgct acgcctgcgt gacaccagcg cggaagcgaa
     5041 acgcgaactg gatgaaatcc gtgaaagttt gcaggttaaa cagagtggct gggcgctgtt
     5101 taaagagaat agcaacttcc gccgcgcggt gttccttggc gtactgttac aggtaatgca
     5161 gcaattcacc gggatgaacg tcatcatgta ttacgcgccg aaaatcttcg aactggcggg
     5221 ttataccaac accaccgagc aaatgtgggg gacagtgatt gtcggcctga ccaacgtact
     5281 tgccaccttt atcgcaatcg gccttgttga ccgctgggga cgtaaaccaa cgctaacgct
     5341 gggcttcctg gtgatggctg ctggcatggg cgtactcggt acaatgatgc atatcggtat
     5401 ccactctccg tcggcgcagt atttcgccat cgccatgctg ctgatgttta ttgtcggttt
     5461 tgccatgagt gccggtccgc tgatttgggt actgtgctcc gaaattcagc cgctgaaagg
     5521 ccgcgatttt ggcatcacct gctccaccgc caccaactgg attgccaaca tgatcgttgg
     5581 cgcaacgttc ctgaccatgc tcaacacgct gggtaacgcc aacaccttct gggtgtatgc
     5641 ggctctgaac gtactgttta tcctgctgac attgtggctg gtaccggaaa ccaaacacgt
     5701 ttcgctggaa catattgaac gtaatctgat gaaaggtcgt aaactgcgcg aaatcggcgc
     5761 tcacgattaa tctcctcagg cttcctcccg tcgcggagga agccgcctct tgcagtcatc
     5821 ttttcttcgc tctatcctct gccgctatga aaacatcccg tctccctatc gccatccaac
     5881 aggccgttat gcgccgcctg cgggaaaaac tcgcccaggc caacctgaag ctagggcgta
     5941 actacccgga gccaaaactc tcttacaccc agcgcggaac ctccgccgga acagcctggc
     6001 tggaaagcta tgaaatccgc ctcaatcccg ttttgctgtt ggaaaacagt gaagctttta
     6061 ttgaagaagt ggtaccgcac gaactggcac atttgctggt atggaaacat ttcggccgcg
     6121 tagcgccaca tggcaaagag tggaagtgga tgatggaaag cgtgctgggc gttcccgccc
     6181 gtcgtacgca tcagttcgaa ctgcaatccg tgcgtcgcaa caccttcccc taccgctgta
     6241 agtgccagga acatcagctt accgtacgcc gccataatcg cgtagttcgt ggcgaggcca
     6301 tctatcgctg tgttcactgc ggtgaacagc tggttgcgaa ataaccacct gaactatcag
     6361 gaactttcct gatctggctg attgcatacc aaaacaactt tcgctacgtt gctggctcgt
     6421 tttaacacgg agtaagtgat gtaccgttat ttgtctattg ctgcggtggt actgagcgca
     6481 gcattttccg gcccgacgtt ggccgaaggt atcaatagtt tttctcaggc gaaagccgcg
     6541 gcggtaaaag tccacgctga tgcgcccggt acgttttatt gcggatgtaa aattaactgg
     6601 cagggcaaaa aaggcgttgt tgatctgcaa tcgtgcggct atcaggtgcg caaaaatgaa
     6661 aaccgcgcca gccgcgtaga gtgggaacac gtcgttcccg cctggcaatt cggtcaccag
     6721 cgccagtgct ggcaggacgg tggacgtaaa aactgcgcta aagatccggt ctatcgcaag
     6781 atggaaagcg atatgcataa cctgcagccg tcagtcggcg aggtgaatgg cgatcgcggc
     6841 aattttatgt acagccagtg gaatggcggc gaaggccagt acggtcaatg cgccatgaag
     6901 gtcgatttca aagaaaaagc tgccgaacca ccagcgcgtg cacgcggtgc cattgcgcgc
     6961 acctacttct atatgcgcga ccaatacaac ctgacactct ctcgccagca aacgcagctg
     7021 ttcaacgcat ggaacaagat gtatccggtt accgactggg agtgcgagcg cgatgaacgc
     7081 atcgcgaagg tgcagggcaa tcataacccg tatgtgcaac gcgcttgcca ggcgcgaaag
     7141 agctaaccta cactagcggg attctttttg ttaaccccta ccccacgcgt acaaccgcgt
     7201 ggggagacga cgcggatttt taactatgcg tatcccccgc atttatcatc ctgaaccact
     7261 gaccagccat tctcacatcg cgctttgcga agatgccgcc aaccatatcg ggcgcgtact
     7321 gcgcatgggg ccggggcagg cgttgcaatt gtttgacggt agcaaccagg tctttgacgc
     7381 cgaaattacc agcgccagca aaaaaagcgt ggaagtgaag gtgctggaag gccagatcga
     7441 cgatcgcgaa tctccgctgc atattcacct cggtcaggtg atgtcgcgtg gtgaaaaaat
     7501 ggaatttact atccagaaat cgatcgaact cggtgtaagc ctcattacgc cacttttttc
     7561 tgagcgctgc ggcgttaaac tggatagtga acgtctgaac aagaagcttc agcagtggca
     7621 gaagattgca attgctgcct gtgagcagtg tggtcgtaac cgggtgccgg aaatccgtcc
     7681 agcgatggat ctggaagcct ggtgtgcaga gcaggatgaa ggactgaaac tgaatcttca
     7741 cccgcgcgcc agtaacagca tcaatacgtt gcctttaccg gttgaacgcg tccgcctgct
     7801 gattggcccg gaaggcggtt tatcggcaga tgaaattgcc atgactgccc gctatcaatt
     7861 tactgatatc ctgttgggac ctcgcgtttt gcgtacagag acaactgcgc tcaccgccat
     7921 taccgcgcta caagtacgat ttggcgattt gggctaacgg agaagaataa tgatcaagct
     7981 cggcatcgtg atggacccca tcgcaaacat caacatcaag aaagattcca gtttcgctat
     8041 gttgctggaa gcacagcgtc gtggttacga acttcactat atggagatgg gcgatctgta
     8101 tctgatcaat ggcgaagccc gcgcccatac ccgcacgctg aacgtgaagc agaactacga
     8161 agagtggttt tcgttcgtcg gtgaacagga tctgccgctg gccgatctcg atgtgatcct
     8221 gatgcgtaaa gacccgccgt ttgataccga gtttatctac gcgacctata ttctggaacg
     8281 tgccgaagag aaagggacgc tgatcgttaa caagccgcag agcctgcgcg actgtaacga
     8341 gaaactgttt accgcctggt tctctgactt aacgccagaa acgctggtta cgcgcaataa
     8401 agcgcagcta aaagcgttct gggagaaaca cagcgacatc attcttaagc cgctggacgg
     8461 tatgggcggc gcgtcgattt tccgcgtgaa agaaggcgat ccaaacctcg gcgtgattgc
     8521 cgaaaccctg actgagcatg gcacttgcta ctgcatggcg caaaattacc tgccagccat
     8581 taaagatggc gacaaacgcg tgctggtggt ggatggcgag ccggtaccgt actgcctggc
     8641 gcgtattccg caggggggcg aaacccgtgg caatctggct gccggtggtc gcggtgaacc
     8701 tcgtccgctg acggaaagtg actggaaaat cgcccgtcag atcgggccga cgctgaaaga
     8761 aaaagggctg atttttgttg gtctggatat catcggcgac cgtctgactg aaattaacgt
     8821 caccagccca acctgtattc gtgagattga agcagagttt ccggtgtcga tcaccggaat
     8881 gttaatggat gccatcgaag cacgtttaca gcagcagtaa cccaccttag cgagaaggat
     8941 ctcgttgaga ctctgagtga cagcgccctt ctttccacgc atactgggcg ctgttgcttt
     9001 tttgaaccag gaaacagaac ctctgacaat gaatttacag catcactttc ttattgccat
     9061 gcctgctctc caggatccga ttttccgtcg ttccgtggtc tacatttgcg aacataatac
     9121 caatggtgca atggggatca tcgtcaacaa gccgctggaa aatctcaaaa ttgaagggat
     9181 tctggaaaag ctgaagatca cgccggagcc gcgtgatgaa tcaatccgtc tggataaacc
     9241 ggttatgctc ggcggcccac tggctgaaga tcgcgggttt attttgcata ctccgccctc
     9301 caattttgct tccagcattc gcatttcaga caacacggtg atgaccactt cccgcgatgt
     9361 gctggaaacg ctcggcaccg ataaacaacc gtctgacgta ttggtggctc tgggctatgc
     9421 ctcctgggaa aaaggtcaac tggaacaaga aattctcgat aacgcgtggc taacggcccc
     9481 ggcggatctg aatattctgt tcaaaacgcc gattgccgac cgctggcgcg aggcggcaaa
     9541 actgattggt gtggatattc tcaccatgcc tggtgtggca ggacacgcct gatgagtgga
     9601 accttactcg ccttcgactt cggcaccaaa agcattggcg tagcggtcgg ccaacgcatt
     9661 accggcaccg ctcgcccttt gcctgcaatt aaagcgcagg acggtacgcc ggactggaac
     9721 ctcatcgaac gtttactgaa agagtggcag cctgacgaaa tcatcgtcgg tttgccgctg
     9781 aatatggacg gcaccgaaca gccgttaacc gccagagcgc gcaaatttgc caaccgtatt
     9841 catggtcgtt tcggtgttga agtaaagctc catgacgagc gtcttagcac tgtggaagcc
     9901 cgttccggtc tttttgaaca gggcggctat cgggcgctca acaaaggcaa agttgactct
     9961 gcctctgcgg ttattattct cgaaagctat ttcgagcagg gatattaagg cgatctaaaa
    10021 gcctggcgga tagtcgttac cgccatcttg ccggatgcgg cgtgaacgcc ttatccagca
    10081 cacatctggc agcggctgta ggtctgataa gacgcgttag cgtcgcatca gacatttatt
    10141 gcctttatag gccctataaa cttgcgcatc gggcatggca acgtcacaaa cgcccttccc
    10201 ccacccgctg ctgataactc tgctgaaacg ttaacatccc caccttctgc ccggtttgaa
    10261 taacatgcgg taactggtgg gttttccctt cgcgaatcaa attccccacc gcgggagtgt
    10321 taatcagtaa ttcaaacagc gccacgcgtc cttcctgttt atccacttcc agcttttgtg
    10381 acagcactgc ccgtaaacta cccgcaagtt gattacgtac gggatctttt tcctgcgccg
    10441 gaaatgaatc caccagtcgc tcaactgcct gcgcggcacc acgtgtatgt aaggttgcca
    10501 gcaccagatg cccggtttct gccgccgtca gcgccagacg gattgtctca ctgtcacgta
    10561 gctctccgag caaaatcaca tcaggatctt cccgcaatgc agcccgcaac cccgatgcga
    10621 acgtcataca gtgcaaacca atttcccgct gctggatcaa acatcgctgg ctggcataga
    10681 gatattccac aggatcttcc agcgtcagaa tatgcgcatc ggcatgttga ttaagatagc
    10741 caaccatcgc cgccagcgtg gtagatttgc cactccccgt agcccccgtc accagaatca
    10801 ggccattctc gctcttgagt aattccggca ataccggtgg tgcaccaagc tgttcgagct
    10861 gtggacagtg cgaaggtaac aaccgtaatg ccagagaaat accttgccgt tgcgcgaacg
    10921 cactgccacg caaccgctgg ttttccgcca gcgacacggc aaaatccagc tggccatttt
    10981 ccagcaatat tgcccgctga tcgtcatcca gccactcccg cagtagctct tcgacgtccg
    11041 gcgcatcaaa cggcgcagct tccattctgc cacgaatgcg ccatcgtgcg ggccaggcgc
    11101 tgcacaggtg tagatccgag acgttatgct ttacactaag ggccacaatt tcttccatat
    11161 tcatactaag atcctcggaa aatgaacgat attgcgcata acctggcaca ggtccgggac
    11221 aaaatctcag cggctgcaac gcgttgcggc cgttctccag aagaaattac gctgcttgca
    11281 gtcagtaaaa caaaacctgc gagcgccatc gcagaagcca ttgatgccgg gcagcgtcaa
    11341 tttggtgaaa actacgttca ggaaggggta gataaaattc gccactttca ggaactgggc
    11401 gtaacaggat tagaatggca ttttattggc ccgttgcagt ctaataaaag ccgcctagtg
    11461 gcagagcatt tcgactggtg tcataccatc gaccgtttgc gcattgctac ccgcctcaac
    11521 gagcagcgcc cggcagaact tccccctctt aacgtactga ttcaaattaa tattagtgat
    11581 gaaaacagta agtccgggat tcaactggca gaactggacg agctggcagc ggcggtcgct
    11641 gaactaccgc gtttacgtct gcgcgggctg atggcaatcc ctgcgcttga gtcagaatat
    11701 gtaaggcagt ttgaagttgc acgccaaatg gctgtagcat ttgccggact gaaaacgcgc
    11761 tacccgcata tcgacacgct ctctctggga atgtcggacg atatggaagc cgccattgcg
    11821 gcaggtagca cgatggttcg tatcggcact gcaatttttg gtgcgcgtga ttactctaaa
    11881 aaataaggaa ttaaaggaac gccatgaata cgttgacttt cctgctttca acggtcattg
    11941 agctgtatac aatggtgctg ttattacgca tctggatgca gtgtgctcat tgtgattttt
    12001 acaccccctt ctcacagttt gtagtgaagg tagcgcagcc aattatcggg ccactgcgcc
    12061 gcgttattcc ggcaatgggt cccattgaca gcgcctcgct gctggttgcc tatattctca
    12121 gttttatcaa agccattgtg ctgtttgaag tggtgacctt cctgccaatc atctggattg
    12181 ccggtttact gattctgctg aaaaccatcg gcttgctgat tttctgggtc ctgctggtga
    12241 tggcgattat gagctgggta agccagggtc gtagcccgat tgaatacgtg ctgattcagc
    12301 tggccgatcc gctgctgcgc ccgattcgcc gcttgctacc ggcaatgggt gggattgatt
    12361 tctcgccgat gatcctcgtt ctgctgctgt atgccatcaa tatgggtgtc gcagaagtat
    12421 tacaggcaac cggaaacatg ctgctgccgg ggctgtggat ggcgttatga gtgccgtaac
    12481 agttaatgat gacggtctgg ttttacggct ctatattcag ccgaaagcca gccgtgattc
    12541 tattgtcggt ttacatggcg acgaagttaa agtcgccatt accgcgccgc cggttgacgg
    12601 ccaggccaac agtcatctgg tgaagtttct cggtaagcaa ttccgggttg ccaaaagcca
    12661 ggtggtgatt gaaaaaggcg aacttggccg ccacaaacaa attaaaatca ttaatccgca
    12721 acaaatcccg ccagaaatcg cggcgttaat taattaggta tcctatgcaa aaagttgtcc
    12781 tcgcaaccgg caatgccggt aaagtgcgtg agctggcgtc gctgcttagc gactttggtc
    12841 ttgatatcgt ggcccaaaca gacctcggcg ttgattccgc tgaagaaacc ggcctaacct
    12901 ttatcgaaaa cgcgattctg aaagcgcgcc atgcggcaaa agtgaccggt ttaccggcaa
    12961 ttgccgacga ctctggtctg gcggtagatg cgctgggtgg cgcgccgggt atttactccg
    13021 cgcgttattc cggtgaagac gcgaccgatc aaaagaatct gcaaaaactg ttggaaacac
    13081 tgaaagacgt accggacgac caacgtcagg cgcgtttcca ctgcgtgctg gtatatctgc
    13141 gtcacgcgga agatccgact ccgctggtat gccacggcag ctggccgggc gtgattactc
    13201 gcgaaccagc gggcactggt ggctttggtt atgatccaat cttcttcgta ccttccgaag
    13261 ggaaaaccgc cgccgaactg acccgcgaag aaaagagcgc catttcccac cgtggtcagg
    13321 cgttgaaact gctgctggac gctttacgta atggttaaat taccgccgct gagtctctac
    13381 attcacatcc cgtggtgcgt gcagaaatgc ccgtactgcg atttcaactc tcacgcgttg
    13441 aaaggagaag tgccgcacga cgattatgtt cagcatctgc tttgcgatct ggacaatgat
    13501 gtggcttacg ctcagggccg tgaagtaaag acaatcttta ttggcggtgg tacgccgagc
    13561 ctgctttccg gcccggcgat gcaaacgctg ctagacggcg tgcgtgcgcg tttgccgctg
    13621 gcagcggatg cagaaattac tatggaagcg aacccaggca cggtagaagc ggatcgcttt
    13681 gtcgattatc agcgtgctgg tgtaaaccgc atctctattg gtgtgcagag ttttagcgaa
    13741 gaaaagctga aacgacttgg gcgtattcat ggcccgcaag aagcgaaacg agcggcaaag
    13801 ctggcgagcg gtttagggtt acgtagcttt aaccttgatt tgatgcatgg actaccggat
    13861 caatcactgg aagaggcgct tggcgatcta cgccaggcca ttgaactgaa tccgccgcat
    13921 ctttcctggt atcaactgac tatcgaacct aatacgctgt ttggctcgcg accgccggtg
    13981 ctgccggacg atgacgcgct gtgggatatt ttcgaacagg ggcatcagtt attaaccgca
    14041 gcgggttatc agcaatatga aacttccgct tacgccaaac ccggttatca gtgccagcac
    14101 aatctcaact actggcgctt tggtgactac atcggtattg gctgcggcgc gcacggcaaa
    14161 gtgaccttcc cggatgggcg cattctgcgt accactaaaa cgcgtcatcc tcgtggtttt
    14221 atgcaaggaa ggtatctgga aagccagcgt gatgtcgaag ccgcagataa gccgtttgag
    14281 ttctttatga atcgcttccg tctgctggag gccgcgccgc gcgtggagtt tattgcgtat
    14341 accgggcttt gcgaagatgt gattcgccca cagttagacg aggcgattgc ccagggttat
    14401 ctcaccgaat gtgcggatta ctggcagata acggaacatg gtaaattatt cttaaactca
    14461 cttct
//
LOCUS       DAEBXN010000102        14346 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.102, whole genome shotgun sequence.
ACCESSION   DAEBXN010000102 DAEBXN010000000
VERSION     DAEBXN010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14346
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.102"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            1..3051
                     /locus_tag="KJL99_003223"
                     /pseudo
     CDS             1..3051
                     /locus_tag="KJL99_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            3061..6707
                     /locus_tag="KJL99_003224"
                     /pseudo
     CDS             3061..6707
                     /locus_tag="KJL99_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            6768..7175
                     /locus_tag="KJL99_003225"
     CDS             6768..7175
                     /locus_tag="KJL99_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4437846.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDVDPLINDALIYIRDEFIDEQKLRGESPEPVIVIFNCKLQNIFNQ
                     QIELYTLDNSSRSTCNLKYDEYSFIKGGNGIPP"
     gene            7717..8805
                     /locus_tag="KJL99_003226"
     CDS             7717..8805
                     /locus_tag="KJL99_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HBD4437847.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATSISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            8816..11095
                     /locus_tag="KJL99_003227"
     CDS             8816..11095
                     /locus_tag="KJL99_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4437848.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQADRRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQEESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDSPGDALNRQREAFMARWIGWAMQQNNGDVLVVCGGWHAPALAKMWRECPQVIN
                     TPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLLEAARHKLEADMLDAGGIGSITTCLSQAASAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGVSLSAAVALLERHSQAIHVLALDRGATLGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DADFTNALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            11088..12225
                     /locus_tag="KJL99_003228"
                     /pseudo
     CDS             11088..12225
                     /locus_tag="KJL99_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708009.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
     gene            12222..14252
                     /locus_tag="KJL99_003229"
     CDS             12222..14252
                     /locus_tag="KJL99_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4437849.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     SALIATFSDGVRTQLANGQALKEAQCTYGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKSITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASVDAFHARASHYHAGECLRQLAALNSRLNCAQEMARSDSVGEVPPVPWRTVVG
                     SGIAGEAKLDHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQEDSP
                     AATRRLFSFQAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPL
                     RQPGIVALREYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEG
                     EDNLLTLSLPASASAPYAIERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKT
                     RAWALDAETAPVVVSLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAINQA
                     ELLANDLTAVGFYRLAHVLAQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
BASE COUNT         3550 a         3769 c         3662 g         3365 t
ORIGIN      
        1 gggattatgc gaataatgtc attattattc tgccgtctgg cattgcacct gactacttgg
       61 agcaatattc cagatttgaa ttacgcctac gtaaacattt atcactggcg gaagagtctc
      121 tctggcaaaa atgtgcacaa aaacttattg ccgcaattcc acatattcca gaatggcggc
      181 aaccattaat tgctttgtta ttacccgaaa aaccagaaat tgcacatgaa attgcccagc
      241 atctactggg gcaaaaaaaa ttaccctcgc ttgagtggtt aaaaatagtg gcgactgatg
      301 agcacattct tgcctcatta gaaaaatatc acgagccata tgccattttt gatgattact
      361 attgtggtgc gatatggtca gccaccgtat tacaggagca aggtgttgca gccctgcccc
      421 gatttgctcc ctatgccgca agtgactact gcgccgatgt gttgcgtcat atcaatcatc
      481 cgttcgcatt aacactgctt atacgtgtag ccgggcatac taaacgctgt cacgatcgaa
      541 tgacgaaagc ctgcgctgcg ttcccacacg cagcactggc agcactggcg gaacttctag
      601 tgcaaaaaga agagaatagt tggcgcatta tgctaatgac gatgtttatc tcgcaaccag
      661 cactggcaga acaggtcatt ccctggcttt cgacacccgc agttgcagtg ctgaaatcat
      721 gccagcacca actgacacag ccctcaaacc atgccagcgc cgatctactg ccagccatag
      781 tagtctcccc accatggatt tcgaaaaaga aaaaatcgcc gattccggtg ctggatttag
      841 cgccattagg catcgagcca atctgttatc tgacagaaga aatcagtaat caacttttgg
      901 cgaaatatat ctggtattca aaacacatca cggttagcca tgaagaaagt actaccaacc
      961 tgttggcaag gatgggtttt caacgacgga tcgctggtac atatattaaa gctcccgaag
     1021 cggtagttga ggcatggcta aatgaagatt attcaacctt actaagtgaa tttaaggtgt
     1081 ttcattcacc taccgggcat tattggcagt tggggatttt gacaacattg ccgctggagg
     1141 ctggagaaag cagtaaaagc atggaatgcc cttactctat ctccacatac cgataccgaa
     1201 tacgccatgt tacattttgg actcaaaggt ttacctggtt tagttaactc aattacacgc
     1261 tatccacagg aagccttgcc catcacgaat tacttcgcag cgagtgagct ggctcctgcc
     1321 gtcgcccgtg ccttcaacaa actcaaaacg ctacgcgaaa acgcccgcac ctggctgttg
     1381 aaatacccgg aacatgccct taccggcctg ctgcctgcgg cgctcggcaa agccggtgaa
     1441 gcacaggata acgcccgcgc tgccttgcgt atgcttaccg aaaacggtca tcagccatta
     1501 ctgcaagaaa tcgcccggcg ttataaccag tcggaagtaa cagacgcggt gaacgctctg
     1561 cttgcgctcg atcccttaga taatcacccg acaaaaatcc ccactcttcc gaccttttat
     1621 cagccatcga tctggacgcg cccggtatta aaagcaaacg cccaatcact gccagataac
     1681 gccctcctcc acctcggcga aatgctccgc ttccctcagg aagaggctct gtatccggga
     1741 ttattgcagg tgaaagacgc ctgtaccgct gactctctgg ctgaatttgc ctgggatctg
     1801 tttaccgcct ggcagaccgc tggcgcgccg tcgaaagaga gttgggcgtt cactgcgtta
     1861 ggcgttctcg gtaacgatga caccgcccgc aaactgacgc cattaatacg cgcctggcct
     1921 ggtgaatccc agcataaacg cgccaccgtt gggttggata ttctcgccgc tatcggtagt
     1981 gatatcgccc tgatgcagct taacggcatt gctcagaaac tgaaattcaa agcattacag
     2041 gagcgggcaa aagaaaagat tgccgacatt gccgagagcc gcgaactcac ggtagcggag
     2101 cttgaagatc ggttagcacc ggatctcggt ctggatgata acggttcgtt gctgctggat
     2161 tttggcccac gccagttcac cgtcagcttt gatgaaacct taaaaccgtt tgtgcgtgat
     2221 gtttccggca gccgcctgaa agacctgcca aaaccaaaca aaagcgatta tgagtcacag
     2281 gcgaacgatg ctgttaaccg ctacaaattg ctgaaaaaag atgcgcgtac cgtcgcggcc
     2341 cagcaggtag caaggcttga atccgccatg tgcctgcgtc gccgctggtc gccggaaaac
     2401 ttccaactct tcctggttgt gcatccgctg gttcgccact taacccgccg tctgatttgg
     2461 ggcgtttata gcgccgacaa ccagctactg gcctgtttcc gggtagcgga agataacagc
     2521 tacagcaccg ctgacgatga tcttttcacc ctgccggaag gcgatatctc tatcggcatt
     2581 cctcacgttc tggaaatatc accgacggat gctgtcgcct ttggtcagct ttttgccgac
     2641 tacgaactgc taccaccgtt ccgtcagctt gaccgtaaca gttacgccct gacagaagcc
     2701 gagcgcaacg ccagcgaact gacccgctgg gcaggcagaa aatgcccgag cggccgggtc
     2761 atggggctgg caaataaagg ctggataaag ggcgaaccac aggatggagg ctggatgatc
     2821 aaacctctcg gtcgctggtc gttaatcatg gaaatcgacg aaggttttgc ggtgggtata
     2881 tcaccagccg aacttagcgc tgagcagctc ttaagcaagc tgtggctatg ggaaggcaaa
     2941 gcggaaagct atggctgggg gagtaattca acacaggaag cgcagttctc cgtactcgat
     3001 gccatcaccg ccagcgagct aattaacgat attgaagccc tgtttgaata aggaaagagc
     3061 atggacaagg aattaccgtg gctggcggat aacgcccaac tggaactgaa atataaaaaa
     3121 ggcaaaacac cactcagtca tcgcaattgg ccgggcgaac cagtgcctgt tatcactgaa
     3181 agcatcattc agacactggg tgatgaattg ctacaaaaag ctgagaagaa aaaaaacatt
     3241 gtctggcgtt atgaaaattt ttcaccggag tggcagtccg ccattacgca ggccatcaac
     3301 ttgatcggcg aacacaaacc ctcagtcccg gcccgcacaa tggcggcact agcctttatc
     3361 gcgcaaaatg acaaccaaca gttgctcgac gaaatcgtcc aacaagaggg gctggaatat
     3421 gcgactgagg tggtgattgc acgccagttt attgcacggt gttatgagag tgatcctctg
     3481 gtagtcacat tgcagtatca gaacgaggac tatggctatg gttatcgctc agaaacctat
     3541 aacgaattcg atctccgact gcgtaaacac ctctctctgg cagaggaaag ctgctggcag
     3601 cgttgcgccg acaaactcat tgccgcactg ccaggaataa ccaaagttcg ccgccctttt
     3661 attgcgctga tcctccccga aaaaccagaa atcgccaatg agttggtaag ccttgaatgc
     3721 ccgcggactc attttcattc taaggagtgg ttaaaagttg ttgctactga ccccaaagcg
     3781 gtgagaaaac tcgaacgcta ctggagccag gatatattta gcgatcgaga agccagctac
     3841 atgtcgcatg aaaaccactt cggctacgcg gcctgcgctg cccttttgcg cgaacaagga
     3901 ctggcagtcg ttccgcgcct cgcgatatat gcccataaag aagattgcgg cagtctgctg
     3961 gtacaaatta accatccgca agtcatccgc accttgctgt tagttgctga taaaaacaaa
     4021 cccagcctgc aacgtgtggc taaatacagt aaaaacttcc cccatgcgac actcgccgca
     4081 ctggcagaac tgctggcgtt aaaagaacca ccagcccgcc caggttatcc aatcatcgaa
     4141 gacaaaaagc tgcctgcaca gcaaaaagct cgagatgaat actggcgtac gctgttacaa
     4201 acgctgatgg catcgcagcc acaactggca gcagaagtga tgccgtggtt aagtactcaa
     4261 gcccgggcag tgctgaatag ttatttatcg gcaccgccca aaccagttat tgatagcacc
     4321 gataacagcc aaatgcctga aatcctcgtt tcaccaccat ggcgtgggag aaagaaaaag
     4381 acttccatac gtcacttttc gctgcctgat cttacgcttg caccgcaaac gtacaggtcg
     4441 ccgaataaac actggacgcc ggaacagcaa actgcaattg accattttag cgccatgtct
     4501 tttgacgaac gtcttgctac gagaggagcg gatatcttct tgcgggaatt aggttttgac
     4561 gttcatagcc tggaatttaa taagcgtctt cttaatggac agttggatgc ctttgatgct
     4621 cagcaactgc aagaatacac atcaatagga ctattgcgta cttatcacgc gcatcaggcc
     4681 aaatctaaac agtaccggga agacgccacc aatgcattac tggcacaaga tagcattgcg
     4741 ttgatgggag ggtggcgaac ttttcagcac ggattttact atggtaatga acagcgttgg
     4801 gggatttgga atctgtacct tatcgcgcag atgccgcgcg aaatggcggt atcgtgctgg
     4861 caacaaattg tcgaggctga ttttcattac accggggtgg aatatttgtt aagtgtgctg
     4921 ggtaccgatg ctttgcctgg attaaacacc gcctttgccc gacatccaaa agagatattt
     4981 ccgctgctga ttcatttcgg cgcaacagaa ctggccctgc ccattgcccg cgtctggcac
     5041 ctttttgcgg gccagcgtaa tctggctcgc cagtggattt tacaatggcc ggaacatacg
     5101 gctactgcac ttatccctct tgtcttcacc aaatctagcg ataaaagcga agctgcatta
     5161 cttgccctgc gtttacttta cgaacatggg catggcgaat ggctacaaac tgtagccaat
     5221 cgttggcagc gtacagatct atggcctgcc ctggaacaat tacttaaaca gagtccaata
     5281 gagatttacc cggcacgcat tccaaaagcc cctgatttct ggcatccgca aatgtggccc
     5341 aggccgcgcc ttatcactaa taatcaacct gttaccgatg acgctctgga aattatcggc
     5401 gaaatgctgc gctttaccca ggggggacgt ttttatagcg ggctggaaca actgaaaacg
     5461 ttctgccagc cacaaacgct ggcagctttt gcctgggatc tcttcactgc gtggcaacaa
     5521 gctggtgccc ccgcaaaaga caactgggca tttctggcgt taagtctctt tggtgacgaa
     5581 agcacggcac gggatctaac gacacatatc ctcgcctggc cacaagaagg caaatctgcc
     5641 cgtgcggtca gtggcatgaa catccttacc ctgatgaata atgatatggc gctgatgcag
     5701 ctgcatcata tatcgcaacg ggcgaaatcc cgccccttac gtgataacgc ggcggaattt
     5761 cttcaggtag tcgcagaaaa tcgcgggcta agccaggaag agttagcgga cagattagtc
     5821 ccaacactgg gccttaatga tccgcaggcg ttgagttttg attttggtcc ccggcagttt
     5881 accgttcgct tcgatgaaaa cctcaacccg gttatctttg atcagcaaaa cgttcgccag
     5941 aaaagtgttc cccggttgcg cgccgatgac gatcaactga aagcgcccga ggcactggcc
     6001 cgactaaaag ggttaaaaaa agatgctact caggtgagca aaaacctgct cccgcgtctt
     6061 gaagctgccc tacgtaccac ccgacgctgg tcgctggcag attttcattc tctgtttgtt
     6121 aatcatccct ttacccgtct ggttacccag cgattaatat ggggggttat ccggcaaatg
     6181 aaccgcgtcg tttactcaac gcctttcgtg tggccgcaga gggggagttc tgcaatgcgc
     6241 aagatgagcc aattgacctg cctgcggacg ctctgattgg cattgcccac ccgttagaaa
     6301 tgacagcaga aatgcgcagt gaatttgcac agctttttgc cgattacgaa attatgccgc
     6361 cttttcgcca gttgtcgcgc cgcacggtgc tgctcacacc tgacgagtca accagtaaca
     6421 gcctgactcg ctgggaaggt aaatccgcta ccgttgggca acttatggga atgcgataca
     6481 aaggctggga gtcaggttat gaggacgcat ttgtctatga cctgggtgag taccggctgg
     6541 tccttaagtt ttcacccggt tttaaccact acaatgttga tagcaaagcg ctaatgagct
     6601 tccgttctct tcgagtgtac cgtgacaata aatccgtcac ttttgccgaa cttgatgtgt
     6661 ttgatttgag tgaggcgtta agcgcacctg acgtcatttt ccattaaaac aaagcgggca
     6721 gtgatgcgac tgcccgttat ctacacgact taccagcggg gaaagcgatg atcgtgcaaa
     6781 aagagctggt tgctatttac gattatgagg tccctgtacc tgaagatccg ttttccttca
     6841 gacttgagat ccataaatgc tctgaattat ttacaggttc cgtctaccga ctggaacggt
     6901 tccggctacg tccaacattt catcaacgtg atcgagagga tgttgacccg ctaataaatg
     6961 atgcgttgat ttatataaga gatgagttta ttgatgagca aaaattacga ggcgaatcac
     7021 ctgaacctgt aatagtaatt tttaattgta aactacagaa tatattcaac caacaaatag
     7081 aattatatac tctagataat tcaagtcgca gcacatgcaa cttgaagtat gacgagtata
     7141 gctttattaa aggggggaac ggtattcccc cttagatgcg tttcaatttg cccgttactt
     7201 caacaaatca atcaaccgta acagagccac taatagctcc agtacagcaa tgagcacggt
     7261 taacttttgt gtcagcgtgt ccatccgctc tccttattaa ggagcacaac accagcacct
     7321 tacccttaca ctgcctgtcg atactggttt acgtttgttg agtcgccatg ttgcactccg
     7381 gatcagagca ctacctgcgg caccacccgt ccggtagcca ggacttgaaa taggttcgat
     7441 ccggcgcaac ttcccaacag tcaggattat aaatctcatt atcttaattg caacactgtc
     7501 cagaggtggt tataattcgt tcgttggaac acgatgttgc acaggcagtg gtgttaggcc
     7561 tgaaaatagt aaataaaaga acctgcctca ccagcagttt tttttattta ctgtgatctg
     7621 ctttccagat atttttcgct caaacaacta atgcgccaaa catttattgc gcgtaaaata
     7681 tcgtttattt cattaataca tttcagggat gaatatatgt caccgcagaa taatcatctg
     7741 cagcgtccgc ctgctgctgt gttatacgcc gatgaactgg caaaattaaa acaaaatgat
     7801 aacgcacctt gcccgcccgg ttggcagtta agtttgcctg cggcccgtgc ttttatcctt
     7861 ggcgatagcg cgcaaaatat tagccgtaaa gtggtgatta gtccctccgc tgtcgaacgt
     7921 atgttagtca ctcttgccac cggacgcggt ttgatgttgg tgggggaacc tggcaccgca
     7981 aaatctctcc tgtctgaatt actggcaacc tctattagcg gcgatgccgg gttaaccatc
     8041 cagggcggcg catctactac cgaagatcaa atcaagtacg gctggaacta cgccctgctt
     8101 atcaatcatg gtccatcaac ggaagcgctg gtccctgccc cgctttacca gggaatgcgc
     8161 gacggcaaaa tcgtccgttt tgaagagata acgcgtacac cgctggaagt gcaagactgt
     8221 ctgttaggta tgctttctga cagggtgatg acggtgccgg aactcactgg tgaagccagt
     8281 cagctctatg cgcgagaagg ctttaatatt attgccactg ccaatacccg cgatcgcggc
     8341 gtcaatgaaa tgagtgctgc gctaaagcgt cgcttcgatt ttgaaactgt ctttccgatt
     8401 atggatttcg cccaggagct ggagcttgtc gccagtgctt cggcgcgttt gctggcccat
     8461 agcggtattc cgcataaagt gcctgacgcg gtactggagt tactggtccg caccttccgc
     8521 gatctgcgtg ccaacggcga aaagaaaacg tcaatggata cactaacagc gattatgtcc
     8581 accgctgaag ccgtgaacgt ggcccacgcc gtaggcgtcc gcgcctggtt tttagcgaat
     8641 cgcgcaggtg agccagccga cctggtggac tgcattgccg gaaccatcgt caaagataac
     8701 gaagaagatc gcgctcgtct gcgccgttac ttcgaacagc gtgtcgccac acataaagaa
     8761 gctcactggc aggcttatta tcaagcccgc caccgcctgc cgtgaggaaa gatgcatgag
     8821 cgagccgtta attgtcggca tccggcatca tagtccggcc tgcgcccggc tggtgaaatc
     8881 gttaatcgaa agccagcggc cacgatacgt gttgattgaa ggccctgctg attttaatga
     8941 ccgggtagac gaactgtttt tagcccacca gcttccggta gctatttaca gttattgcca
     9001 gtatcaggac ggtgcagccc ccgggcgtgg tgcctggacg ccatttgctg aattttcgcc
     9061 ggagtggcag gcgctacaag ccgatcgtcg tattcaggca caaacttact tcatcgattt
     9121 gccttgctgg gcgcagagtg aagaagagga cgattcgcct gatacgcaag aggaaagcca
     9181 gaccttgctg ctgcgtgcca cccgcatgga taacagcgac accctgtggg atcacttgtt
     9241 cgaagatgaa agccagcaaa ctgcattacc ctctgcactg gcgcactatt ttgcccaact
     9301 gcggggcgac tcccccggcg atgctctaaa tcgtcagcgc gaagccttta tggcccgctg
     9361 gattggatgg gcgatgcagc aaaataatgg cgacgtgtta gttgtctgcg gtggctggca
     9421 cgctccggca ctggcaaaaa tgtggcgcga atgtccgcag gtaattaaca cgccagaatt
     9481 gccgtcgctg gcagatgccg ttacaggttg ttatctcacc ccctacagtg aaaagcgcct
     9541 tgatgtgctg gcaggatacc tttcaggaat gcctgccccg gtctggcaaa actggtgctg
     9601 gcagtggggc ttacagcagg cgggagaaca actgctaaaa acggttctca cccgtttgcg
     9661 ccagcacaag ttgcctgctt cgaccgcgga tatggctgcc gctcatctgc atgcgatggc
     9721 actggcacag ttgcgcggtc atacactacc gttacgcact gactggctgg atgccatagc
     9781 aggatcgctg attaaagaag ccctgaatgc gccgttgccg tggagctatc gcggcgttat
     9841 tcatcccgat accgatccga ttctgctaac gttgatagac acattagcgg gtgacggatt
     9901 cggtaaactt gccccttcta cgccacaacc gcctctgcca aaagatgtca cttgcgaact
     9961 ggaacgtacc gcaatctctc ttccggcgga gcttacctta aatcgcttta accccaatgg
    10021 actagcgcaa agtcaggtgt tacatcggct ggcaatactg gagatcccag ggattgtacg
    10081 ccagcaggga agcacactga cacttgcagg caacggtgaa gaacgctgga aattaacccg
    10141 cccgcttagc cagcatgcgg cattgattga ggccgcctgc tttggtgcca cactcctgga
    10201 agccgcacgc cataaattag aagccgatat gctggacgcg ggtggaatcg gcagcattac
    10261 cacatgtctt agccaagcgg cgtcagcggg tctggcgtcc ttcagtcaac aattactgga
    10321 gcaactcaca ttattaatcg cccaggaaaa tcaatttgcc gaaatgggcc aggcgctgga
    10381 agtgctatat gccttatggc ggctggatga aattagcggt atgcaaggcg cgcagatatt
    10441 acaaacgacg ttatgcgcgg ctatcgatcg cacgctgtgg ctgtgtgaat ccaacggcag
    10501 accggatgaa aaggagtttc acgctcactt gcatagctgg caagcgttgt gtcatatcct
    10561 gcgcgatcta catagcggtg tgcaactccc cggcgtttcg ctttctgcgg cggtagcctt
    10621 gctggagcga cacagtcagg cgattcatgt cctggcactg gatcgcggcg cgactcttgg
    10681 tgcgctgatg cgtctggaac atcccaacgc tagtgccgaa gcggcgctga cgatgctggc
    10741 gcagttatcc ccggcacaat ccggcgaggc gctgcacggt ttgctggcat tagcccgtca
    10801 tcaactggcc tgtcagccgg catttatcgc cggtttcagc agtcatttaa atcaactaag
    10861 tgatgccgat tttaccaatg ccctgcccga tttacgcgcg gcaatggcct ggctaccgcc
    10921 acgagaacgc gggacgctgg cgcatcaggt gcttgagcat tatcaactgg cgcaacttcc
    10981 cgtttcggca ctgcaaatgc cgttgcattg tccaccgcaa gccattgcac atcatcaaca
    11041 actcgaacag caggcactgg catcgctgca acactgggga gttttccatg tctgaactga
    11101 acgatcttct gaccacccgt gagctacaac gctggcgatt aattcttggc gaagcggcag
    11161 aaacgacgct ttgtgggctg gatgacaacg cccggcagat agatcacgcg ctggagtggc
    11221 tgtatgggcg cgatcctgaa cggctccagc gtggtgaacg ctctagtgga ttaggtggct
    11281 caaatctcac cactcctgag tggatcaaca gtattcacac gctgtttccg caacaggtga
    11341 ttgagcggct ggaaagcgat gccgtactgc gctacggcat tgaagatgtg gtgacaaatc
    11401 tcgacgtgct ggaacgtatg cagccttccg aaagcctgct acgcgccgtt ttgcacacca
    11461 aacatctgat gaaccccgaa gtactggctg ccgcccgcag gattgtgcgc caggttgtgg
    11521 aagaaattat ggcgcgactg gcaaaggaag ttcgtcaggc tttttctggt gtccgcgatc
    11581 gtcgtcgccg ctcatttatt ccactggcgc gaaactttga tttcaaaagt actctgcgcg
    11641 ccaacctgca acactggcac ccgcaacacg gcaagttgta tatcgaattc ccccgcttta
    11701 acagccgcat taaacgccaa agcgagcaat ggcaactggt cttactggtt gatcaaagcg
    11761 gatcgatggt cgattcggtg atccactctg cggtgatggc ggcctgttta tggcagttac
    11821 ccggcattcg tacccatctg gtggcgtttg accacaagcg tcgttgatct cacggcagac
    11881 gttgccgatc cggtagagtt attaatgaaa gtgcaattag gcggcggaac caatatcgcc
    11941 agtgccatgg agtatggtcg gcaacttatt gaacaacctg caaaaagcgt cattatcctc
    12001 gtgagcgatt tttatgaagg gggttcatca tcattgctga cgcaccaagt gaaaaagtgt
    12061 gtccagagcg gcatcaaagt gctgggactg gcagcgctcg atagcacagc aacaccttgc
    12121 tatgaccgcg atacggccca ggcgctggtt aatgtcggcg cacaaatagc cgccatgaca
    12181 ccgggcgagc tggcatcatg gcttgcggag aatcttcagt catgaattca ctacgtccgg
    12241 aattattaga actgacaccg caggcactga cggcgttaag caatgccggt tttgttaagc
    12301 gcagccttaa ggaactggaa aatggcaacg tcccggaaat cagccatgag aacagcgctt
    12361 taatcgccac cttcagtgac ggtgtccgta cccagctggc gaacggccag gcactgaaag
    12421 aggctcagtg cacctatggg gccagcggca tgtgccgtca tcgcgtgatg ctggtgttaa
    12481 gttatcaacg actttgtgct accgcccagc ctacgggaaa agaagaggaa tgggatccgg
    12541 caatctggct ggaagaactg gctacccttc ccgatgccac ccgcaaacgc gcacaggcgc
    12601 tggtcgctaa aagcatcacc attgagttgt tctgtgcgcc gggtgaaatt ccctctgccc
    12661 gcttaccgat gagcgatgtg cgtttttatt cccgcagcag tattcgtttc gcccgttgtg
    12721 attgtattga aggcacactt tgcgaacatg tcgtactggc agtacaggcc tttgtccagg
    12781 caaaagcgca gcaagcggaa ttgactcatt taatctggca gatgcgcagc gaacacgtca
    12841 catcatctga cgatccgttt gccagcgaag aaggcaaaac gtgtcgtcaa tatgttcaac
    12901 aactaagcca ggcattatgg ctgagcggca tcagccagcc gcttattcac tacgaggccg
    12961 ctttcagccg cgcgcagcaa gcagcggaac gctgcagctg gcgatgggtg agtgaatcgc
    13021 tacggcaact gcgcgccagc gttgatgcct tccacgcccg cgccagcgtt gatgccttcc
    13081 acgcccgcgc cagccactat catgccggag aatgcttacg tcagcttgcg gcattaaaca
    13141 gtcgattaaa ttgcgcacaa gagatggccc ggagcgacag tgttggtgaa gttcctcctg
    13201 tgccgtggcg cacggtcgtt ggctctggca ttgccggaga agcaaagctt gatcatctgc
    13261 ggctggtgtc tttaggtatg cgttgctggc aggatattga gcattatggt ttacgcatct
    13321 ggtttaccga tcccgacacc ggcagtattt tgcacctttc gcgcagttgg ccgcgaagtg
    13381 aacaggaaga ctcaccggca gctacgcgtc ggctgtttag ttttcaggct ggcgcactgg
    13441 cgggtgggca aattgtttca caagcagcaa aacgcagtgc cgatggcgag ctgctgttag
    13501 ctacccgcaa ccgcttaagc agcgttgtac cgctgtcgcc tgatgcctgg caaatgttga
    13561 gcgcgccgtt acgccagccg ggcattgtgg ctttgcggga atatttacgc cagcgtcccc
    13621 ccgcctgcat acggcctctt aatcaggtcg ataacttatt tattctgccg gtcgctgagt
    13681 gtatttcgct cggttgggat agcagccgcc agacgctgga tgcgcaggta atcagcggcg
    13741 aaggtgaaga taatctgctg acgttatcac tgccagcctc agccagcgcg ccttatgcca
    13801 ttgaacgcat ggcggcgctt ttgcaacaaa cagacgaccc cgtgtgtctg gtttctggct
    13861 ttgtcagttt tgttgatggg caattgacac tggaaccaca ggtaatgatg acaaagactc
    13921 gtgcctgggc gctggacgca gaaactgcgc ctgtggtggt atcactacct tctgccagcg
    13981 ttctgcctgt gccgtctact gctcatcagt tgctgatgcg ctgccaggcg ttacttattc
    14041 aactgctcca taacggctgg cgctatcagg aacagagcgc tattaatcag gcagagttgc
    14101 tggcgaatga cctcaccgcg gtgggttttt atcggctggc acatgtgttg gcacaatttc
    14161 gtaatacaga aagcgaggca cgggtagaag caatgaataa cggtgttttg ctttgcgaac
    14221 aattattccc catgcttcag caacaaggat gaaatagtgc tttttactaa gagttctact
    14281 ccagttccgg actgctcacg ccacggtatt aggcatatcc tatatagccc ctggtgagag
    14341 tcacca
//
LOCUS       DAEBXN010000103        14177 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.103, whole genome shotgun sequence.
ACCESSION   DAEBXN010000103 DAEBXN010000000
VERSION     DAEBXN010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14177
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.103"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(365..982)
                     /gene="tdk"
                     /locus_tag="KJL99_003230"
     CDS             complement(365..982)
                     /gene="tdk"
                     /locus_tag="KJL99_003230"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HBD4437850.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1587..2000
                     /gene="hns"
                     /locus_tag="KJL99_003231"
     CDS             1587..2000
                     /gene="hns"
                     /locus_tag="KJL99_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HBD4437851.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2144..3052)
                     /gene="galU"
                     /locus_tag="KJL99_003232"
     CDS             complement(2144..3052)
                     /gene="galU"
                     /locus_tag="KJL99_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBD4437852.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="KJL99_003233"
     CDS             complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="KJL99_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HBD4437853.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="KJL99_003234"
     CDS             complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="KJL99_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HBD4437854.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5377..5835
                     /locus_tag="KJL99_003235"
     CDS             5377..5835
                     /locus_tag="KJL99_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707139.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HBD4437855.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMTGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5885..6727
                     /gene="purU"
                     /locus_tag="KJL99_003236"
     CDS             5885..6727
                     /gene="purU"
                     /locus_tag="KJL99_003236"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HBD4437856.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6887..6971
                     /locus_tag="KJL99_003237"
     tRNA            6887..6971
                     /locus_tag="KJL99_003237"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6921..6923,aa:Tyr,seq:gta)
     gene            7018..7149
                     /locus_tag="KJL99_003238"
     ncRNA           7018..7149
                     /ncRNA_class="other"
                     /locus_tag="KJL99_003238"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7333..8010)
                     /gene="narI"
                     /locus_tag="KJL99_003239"
     CDS             complement(7333..8010)
                     /gene="narI"
                     /locus_tag="KJL99_003239"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HBD4437857.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRMFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="KJL99_003240"
     CDS             complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="KJL99_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HBD4437858.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQVQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8717..10255)
                     /gene="narH"
                     /locus_tag="KJL99_003241"
     CDS             complement(8717..10255)
                     /gene="narH"
                     /locus_tag="KJL99_003241"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HBD4437859.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10252..13995)
                     /locus_tag="KJL99_003242"
     CDS             complement(10252..13995)
                     /locus_tag="KJL99_003242"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HBD4437860.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGYLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
BASE COUNT         3502 a         3749 c         3402 g         3524 t
ORIGIN      
        1 aagggccttc ccgagagtgg taaaggagag gccctggaaa taccccacag ctccgaaaaa
       61 gagccagtca gttgcttaac tgactggcat taccccttct ggggccgttt ttttgtttac
      121 ccaaagcaac ttttccataa accgacagca ttagccttca tcatatttgc gacgatgtat
      181 aacgcctaaa cacagggata ttgtacttta caggtcacaa gtcaacgtcg gtgcttaaga
      241 gccctgtgag gcgtatagcg gcgttaaaaa actgctgaga agggtatata gcccggaaga
      301 agtgcgtaaa acgaactgac aggataaaag tgccctgctc accctgtcag taaagaaatt
      361 cttattaatc gtggcgatgc ctttcctgaa tagccgttaa tgagccgact tgtaacgcct
      421 ctttatagtg tttacggcat acagaaacgt atcgttcatt accaccaatt accacctgct
      481 caccttcgtt ataaggtctg cctgcttgat caagacgcag caccatgctt gctttacggc
      541 cacaaaaaca gatggttttt aattcaacca gtttgtcgga ccatgccagt aagtattggc
      601 tgccaataaa taattcacct cgaaaatcgg tacgtaaacc ataacaaagt acgggtatat
      661 cgagttgatc gacaacctcc gataattcat atacttgttg tctggttaaa aactggcatt
      721 catcaaccag tacgcaatga attgcctgct gttcatgttc cgcacgaatc tcatcaaata
      781 atgatgaatt ttggttaaat aattttgcag gcgatgacaa acctatacgc gaactgactt
      841 tcccggcacc aaagcgatca tcaatttctg ccgtatatac gacagtgcgc atgccgcgtt
      901 cctggtaatt gtatgaagat tgcaacaatg ctgtagactt acccgcattc attgcggaat
      961 agtagaaata tagctgtgcc atcagccaca ggccctcaat gatatgaaat aaagttggct
     1021 ggagtttatc ataattcgtc agtttttcag ctcaacagta tgcacagaag tgggtatatg
     1081 cgttctccct tacgaagcct tgcataatcc ttctgagcta tcattacaac tgcctcgctt
     1141 gttataagcg ggtaaatgac tgctggtaac tattcacaat ctttaacctg ttgcgcaagt
     1201 aatagccctc tgttgacctc caggagatag tgcaatacta agtccatgct cttattgcga
     1261 ctgttctact tttcatcatt cgcttaatag ggaattctcg taaacacaac taatacagaa
     1321 gactgaaagg tcgtcagcct acgataatct ccccataaaa tgtgacatga atcaggaagt
     1381 tttaacctca cgtgctgcga aatcatcggt gtaaataggg ctatatgccg cgtcttttct
     1441 ggctaatttt atgaaaagat atttattggc ggcacaaaat aaagaacaat tttgaattcc
     1501 ttacattcct ggctattgca caactgaatt taaggctcta ttattacctc aacaaaccac
     1561 cccaatataa gtttgagatt actacaatga gcgaagcact taaaattctg aacaacatcc
     1621 gtactcttcg tgcgcaggca agagaatgta cacttgaaac gctggaagaa atgctggaaa
     1681 aattagaagt tgttgttaac gaacgtcgcg aagaagaaag cgcggctgct gctgaagttg
     1741 aagagcgcac tcgtaagctg cagcaatatc gcgaaatgct gatcgctgac ggtattgacc
     1801 cgaacgaact gctgaatagc cttgctgccg ttaaatctgg caccaaagct aaacgtgctc
     1861 agcgtccggc aaaatatagc tacgttgacg aaaacggcga aactaaaacc tggactggcc
     1921 aaggccgtac tccagctgta atcaaaaaag caatggatga gcaaggtaaa tccctcgacg
     1981 atttcctgat caagcaataa tcttttgtag attgcacttg cttaaaatcc cgccagcggc
     2041 gggatttttt attgtccggt ttaagacaat ttaataagaa taagctataa aaaaacggcg
     2101 tcgattgctc aacgccgttt cgtggataac accgatacgg atgttacttc ttaatgccca
     2161 tctcttcttc aagccaggct ttaaattccg tgccaagggt gttatgacga ataccgtatt
     2221 caacgaaggc ctgcatgtaa cctaatttat taccgcagtc atggctcttc cctttcatat
     2281 gataggcttc caccgtttct ttttcgatca gcatatcaat tgcgtcggtg agctgaattt
     2341 catcaccagc tcccggaggg gtttttgcca gcaacggcca aatatccgcg ctaagtacgt
     2401 aacgacccac aatagcgaga ttagacggcg caacatccgc tttcggtttt tctaccacac
     2461 caaccatcgg tacgctttca cccggcgcta attcaacgcc tttgcaatcc acaacgccat
     2521 atgcggtcac atcagcaacc ggttcaacca tgatctggct atgacccgtt tcatcaaagc
     2581 ggcggatcat ctctgccagg ttatcctgtg acaaatcgga ttcatattca tccagaataa
     2641 catcaggcaa aataacagct accggttcat cacccactac cgggtgagca cacaataccg
     2701 cgtgtcccag gcctttcgcc agaccctgac gaacttgcat aatagtcacg tgcggtggac
     2761 aaatagactg cacttcatca agcagttgac gttttacacg tttttccagc attgcttcca
     2821 gttcaaaact ggtatcaaag tggttttcaa tagagttttt agatgagtgt gtaaccagca
     2881 caatttcagt aatgccagcc gcaatacatt cattcacgac gtattgaatt aatggcttat
     2941 cgacaagtgg cagcatctct ttcgggatgg ctttcgtcgc cggcaacatc ctggttccta
     3001 atcccgcaac ggggataacg gcttttttga ctttcgtatt aatggcagcc atttaaattc
     3061 tcctggactg ttcgtgtttt gaacgtgttc atatttctgt atcgcatccc agtatatcag
     3121 caccccgaag gggctaagtt ctgaaaagga ggcgagatag cataattaag acaattactt
     3181 atacgaatgg cgacgatagt accaccagcc gacattagca ggtaatgcaa atttagcccg
     3241 cgttatcgtt tgctcattct gcagacaaca tcaagcgcag tcgaccaccg gttccccata
     3301 tttggcattg ccaggcatcg catcgctggc tcaattgatt caaataagcg ttacctaaag
     3361 tgcctaacgg aacaccatta ctgatttgca cctggtgttc gccggtattt aacgtcgcat
     3421 tcagacccgc agaaaccaga atgagatttt tcagttcgcg atgataatag ccaactaata
     3481 gcggaaactg ccccggcaga ttggcctgac gaagtaaatg gtttacctgc ttcaataacg
     3541 cgcccaactc tggcaaccgt tgattttggt gtgcaagctg ttcctgtaat aatccgttaa
     3601 acaatgcgcg taataacaag gcagcaagta cgccattatg tccagctcgg gtgacatcaa
     3661 ggcaataaaa tgccagatcg ttttccgaaa gtgcggcaat atcaagcacc aggccgggtt
     3721 tgtccgcggc aaccaattga cggtaattaa cccggcaatg ggaaatcacc tgctgaaccg
     3781 gcggttgtag ttcctgtaat aatttcgccg ctgcggcagg gttatcaacc attgcatccc
     3841 agtcgcgaaa aagcctttcc tcttcctcaa cgcgcgaatt aaacatgctg ggatagagac
     3901 aggcaaaaac catctcgcgc aagcgattca gatctttaac tggtttcagc aaaacatctt
     3961 caacgcccag acgtaacgct ttggcaatat ctgccatatt ttcagtggca gatatcacca
     4021 gaactggggt ctggtcgcct ctgttacgta tatgctccag cagtttaagc ccgttcattc
     4081 gtggcatcgc gatatcacat atcatcaggt ctggagtgaa acctcccagc aactcaaggg
     4141 catccacccc atcagccgcc agtaccgttg tcgctcccaa tgaggaaaac catgaatcca
     4201 gaagcgagcg aaatacctgc tcatcttcaa caatgagaat ctgttttccg accaatggct
     4261 gcgtcatgtt ctctcccctg actggcttta ctcaatagtg gcatgctatt gccacctgcg
     4321 cctgtcagaa ttaacttaag tgtagataaa aattctggtc aaatgttggt gcgtaccaac
     4381 ggcaaaagtt cgtccatttt cctttccact gccagctgtc cggccgcaat ggcagcgtgc
     4441 gcgcgatgga aatcaagcgt agatatttgc gggcaaacag gttgaatcag aatatcgggc
     4501 ggatcacctg ccatgcggtt ccttttaagg cggttctcca acacctggat agacgtggtc
     4561 ataatctctg ttgccgttgg cgctgtcaca gcccgacgtg tcgttatgct gcctaacctt
     4621 tctttcagac gcgcatgcca cggtagagaa tcaccattct cgctattttc ttcactgaca
     4681 ttaaaggaga gcagatcttg ttgcatcaaa tgagcatcgt gctgcaggtc gaccgctatc
     4741 acaatatcag cccccaatgc acgcgtaaga gaaataggaa ttgggttaac tactgcccca
     4801 tcaaccagcc agtagccgtt atgtgcaacg ggtgccatga gtcctggaat actgcatgat
     4861 gcgcgaatag caagatggag atcgccttca gtaaaccata attcacgccc cgtacttaaa
     4921 ttggtggcaa cagccgcaaa gcgacgggaa caattttcga tctctgtttc cggcattatt
     4981 tcgcgatatt gattgaagac acgctcgccg cgcagtaacc caccgcgctg ccaggagagg
     5041 tccatcaggc gtaaaacgtc ccaataactg aaagaggtta cccaatcttc cagcgcagat
     5101 aatcgatcgc atgcataggc cgcgcccacc agcgaaccaa ttgaacatcc tgcaacgata
     5161 tcaatttcaa tacccacttt ttttagcgca ttaataacgc caatatgcga ccaacctctc
     5221 gccgcgccag atcccagcgc cagccctatt tttatctttc tcattattcc cgcaagattc
     5281 ccctggaatg caatcgtagc cacatcgcta ctgctcggtt aacatagtgc tactcaggcg
     5341 gttaatacgc cgttattgtt tccagggaga ttatttgtgt ctcagctttg tccctgtggt
     5401 agtgctgtcg agtatagcct atgttgccac ccttatgtgt ctggtgaaaa ggttgcacct
     5461 gatccagaac atctcatgcg ttcgcgttac tgcgcttttg tgatgcaaga cgctgattat
     5521 ttaataaaga cctggcatcc ctcttgtgga gcagcggcgt tacgtgccga attgatgacc
     5581 ggatttgcgc atactgaatg gttgggattg accgtatttg aacattgttg gcaggacgcc
     5641 gacaatatcg gatttgtcag ttttgttgct cgatttaccg aaggtggcaa aacaggtgca
     5701 ataattgaac gttcgcgatt tttgaaagaa aacggtcaat ggtactatat tgacggtaca
     5761 cgtccgcagt ttggtcgcaa cgatccctgc ccttgtggtt caggtaaaaa atttaaaaag
     5821 tgctgcggcc aataatggtt gacggtacgg tttagcaaac actctcaaca aggtttttcc
     5881 agcaatgcat tcactccaac gtaaagttct gcgtactatt tgtccggacc aaaaaggtct
     5941 gatcgcacgt attaccaata tttgctacaa gcacgagtta aatatcgtac agaacaatga
     6001 atttgttgat caccgtaccg ggcgcttttt tatgcgcacg gaactggaag ggatttttaa
     6061 tgattccacc ctgctggcgg atctcgatag cgcattgcca gaaggctccg tgcgtgagct
     6121 gaatcctgcc ggtcgtcgcc ggatagtgat tctggtcact aaagaagcgc attgccttgg
     6181 cgatttgttg atgaaagcca attacggcgg tctggatgtc gaaatcgcgg cagttattgg
     6241 taaccacgat actttacgtt ctctggttga gcgttttgat attccgtttg agctggtaag
     6301 ccatgaaggg ttaagccgca acgagcacga tcaaaagatg gcggacgcca ttgatgctta
     6361 tcaacctgac tatgtggtgc tggcgaagta tatgcgggta ttaacgccgg aatttgtggc
     6421 acgcttcccg aataaaatca tcaatattca ccattcattc ctgcctgcgt ttatcggcgc
     6481 acgtccttat caccaggcct atgaacgtgg tgtgaagatt attggcgcaa ccgctcacta
     6541 tgtgaatgac aatctggacg aaggcccaat catcatgcag gacgttattc atgtcgatca
     6601 tacctacaca gctgaagata tgatgcgcgc aggtcgtgac gtcgagaaaa acgtcttaag
     6661 tcgtgcgcta tacaaagtac tggcgcaacg cgtctttgtt tacggtaatc ggacgattat
     6721 tctttaatcg ccagcaaaaa taactggtta cctttaatcc gttacggatg aaaattacgc
     6781 aaccagttca tttttctcaa cgtaacactt tacagcggcg cgtcatttga tatgatgcgc
     6841 cccgcttccc gataagggag caggctagta aaaagcatta ccccgtggtg gggttcccga
     6901 gcggccaaag ggagcagact gtaaatctgc cgtcatcgac ttcgaaggtt cgaatccttc
     6961 ccccaccacc atcactttca aaagccccgg aattctcaaa cgaatctgca atcaaatatt
     7021 ctgcccaagc ggggaaggat gagaagcttc gaccaaggtt cgacttgagc gccagcgaga
     7081 gagcgttgcc gcaggcaacg acccgaaggg cgaagcgcgc agcgctgagt aatccttccc
     7141 ccaccaccat cgcttccttg aataatcaaa acctgttact ccgagctaat tttgcccgat
     7201 ggcaccacgt ttaccgggcc tacaatatcc tcccctgtag gtcggataag cgaagcgcat
     7261 ccgacaactc accccgcacc gtgcccataa aaaaaccccg ccgaagcagg gtctatgtga
     7321 actaaaattc gtttagtgac gagcgcgcac cagctggtac ttacgtgtca gatactccac
     7381 cggtacgctc cagatgtgta ccagacgcga gaacgggaac agcaggaata acgtcatccc
     7441 cagcaccagg tgcagacgga agataaacgc cacaccatcg aggtgttgag aagcgccacc
     7501 gtggaaggtc accaccgact gcgcccagcc aaccagtttc atcatctcgc taccgtccat
     7561 atgctgagcg gagaacggaa tggtcagcag gcccagcgcg cactggataa cgagcagcga
     7621 caggatcagg atatccgctc cggtagtggt tgcacgcacg cgtgggctga acatacgacg
     7681 tttcagcaaa agcacgccgc caatcagaca cagcacgccg ctggcaccac cagcaaacat
     7741 cgccattttc tgtttcactt caatcggcag ccaggcttca tacatccagt gcggcgtcaa
     7801 cataccgaag aagtgaccga caaaaatccc cagaataccg atatggaaca ggttcgacgc
     7861 caggttcatc cctttgcgat ccagcatctg gctggacgcc gcgcgccagg tgtactgccc
     7921 gtagtcataa cgcagccagc taccaatcag gaagaccgcc ccggctatgt acgggtagat
     7981 atcaaagaag aacatattca ggaattgcat tagtgctgtc ctccggtggt gatattcaga
     8041 tattgcggcg cgacggcacc ggcaaagcga cgctgatgag cagtgattgc tgaatcaccg
     8101 cagcctttgt cagcaaagaa tttaacctgc tcttcttccc agacagcatc cagcgcctgc
     8161 ggcgtatcat cgcgcgcttc gtcggcaatt ttttccgcca ctttgtcgct gtcgatagcg
     8221 gtattggcca atttcagcag cagatcaaac atcacggcat aacggctttc acgctgttgc
     8281 agacgcgcgc tcagcaatgc cagaatcggc gcgatatctt tcaaaccttc cacggcttcg
     8341 ctttgcggca gctgcgacag gtactccaga tacagcggca gatggtccgg taattcgcgg
     8401 ctgtttaact gcaagccgtg ctgctcgtac tgcgccagca ggtccaccat cgcctggccg
     8461 cggtcacggg attcaccgtg aacatgttca aatagcaaca gtgacgtggc acggccacgg
     8521 tcgaacagtt cgctgtactg gacctgggca tcgagcggat ccatcgccgt taaatcgcgc
     8581 aggaaaatgc ccagcgcatg ggcatcttct ttcgacagat ttttcgacgc agcaatcgcc
     8641 tcaaacatct cttgttgatg ctgccataag gcagcatccg gatattcaag gagacgcgat
     8701 acaatcacga gttcgatcat ggatgcggct ccgttttgct ggtcacatcg atggcatcga
     8761 tacgacggct gttgaacaga ttgaatttgg tatctgagcc gtggcagcca tcaccaaagg
     8821 taaagccgca gccatttttc tccgggaagg cttcccgtgc cagttcacga tgactactcg
     8881 gcaccacaaa gcgatcttcg tagttagcaa tcgccagata acggtacatc tcctgtgcct
     8941 gggcttcggt cagaccgacc tcttccagcg cacgggtatc aactttaccg tcaacagttt
     9001 cagcacgttt gtaatgacgc atcgccagca tacgtttcag cgcacgcagt accggtttgg
     9061 tatcaccggc ggtcagcaga ttcgccagat actgtaccgg aatacgcaag ctttcgacgt
     9121 ctggcagaat tccgttgctg cccagctcgc ccgcgtctgc tgcagactga atcggagaca
     9181 gaggcggcac gtaccagacc atcggcagtg tgcgatattc tggatgcagc ggcagcgcca
     9241 gtttccattc catcgccatt ttataaaccg gcgactgctg tgcagcttca ataacgctca
     9301 gcggaatacc gtctttaatc gcctgctcga tgactttcgg atcgttcgga tcgaggaaca
     9361 cgtccagctg acgctggtag agatctttct cgttctcggt gctggctgca cgttcaatcg
     9421 cgtcggcatc gtacagcagc acgccaagat aacggatacg accgacacag gtttctgagc
     9481 acacggtcgg ctgacccgct tcaatacgcg gatagcagaa gatgcacttc tcagacttac
     9541 cgctcttcca gttgaagtag atttttttgt acgggcatcc ggtgatgcac atacgccagc
     9601 cacggcattt atcctggtcg atcaggacga tgccatcttc ttcacgcttg taaatcgcac
     9661 cgctcgggca ggtcgccaca catgccgggt tcaggcagtg ttcgcacagg cgcggcaaat
     9721 acatcataaa ggtgttttcg aactggctat acatcgcctt ctggatgttg tcgaagttct
     9781 tgtctttcgc cagtttatca aactcaccgc ccaggtcatc ttcccagttc ggcccttttt
     9841 cgattttcgc catccgttcc ccggtaatca gcgaacgcgg acgggcaatc ggctgcgatt
     9901 tgctgccttc cggcgcggta tgcaggttct gataatcaaa atcgaacggc tcgtaataat
     9961 cgtcgatccc cggcagatgc gggttagcaa agattttacc cagcagcatg gctcggttac
    10021 ccatgcgcgg ttgcagtttg ccattgattt tacggatcca gccgcctttg tatttttcct
    10081 ggttttccca gtcagtcggg aagccctggc ccggcttggt ttccacgttg ttgaaccacg
    10141 cgtattccac gccttcacgg ctggtccaga cgtttttaca ggtaactgaa caggtgtggc
    10201 agccgatgca cttatcgaga ttcagcacca tgccgacttg tgaacgaatt ttcattttac
    10261 gctctcctgt acctggtcat tgccttcgcc atctaaccag tcaatgttct tcatcttacg
    10321 cactacaaca aactcatcgc ggttagaacc tacggtgccg tagtagttaa agccgtatgc
    10381 cagatgggca tagccgccga tcatatgcgt cggtttcggc gtgatacggg tgaccgagtt
    10441 atggatacca ccacgctgtt gggtaatttc cgaaccaggc aggttaacga tacgttcctg
    10501 cgcgtggtac atcatggtca tccctgccgg aacacgctgg ctgacaaccg cacgggcagt
    10561 cagtgcaccg ttgctgttga agacttcaat ccagtcgtta tcggcgatac ccagatcttt
    10621 ggcatcggct tcactcaacc agaccaccgg accaccgcga cctaaagtca gcatcagcag
    10681 gttgtcgctg taggtggagt ggatacccca cttctggtgc ggcgtcagga agttgagcgc
    10741 tttttccggg ttgccgttgg atttctggcc tatcacttct ttcaccgaac gggtgtcgat
    10801 cggcggacga taaaccagca ggctttcacc gaaatcacgc atccactggt gatcctgata
    10861 cagttgctga cgaccagaga gcgtacgcca tgggatcagc tcgtgaacgt tggtgtaacc
    10921 ggcgttgtaa gaaacgtgtt catcttccag accagaccag gtcgggctgg agataatttt
    10981 gcgcggctgt gcctgaatat cgcggaagcg gatcttctcg tcttctttat tcagcgccag
    11041 atgcgtatgg tcacgaccgg taaattcgct cagggcagcc caggctttca cggctacctg
    11101 accgttggtt tccggtgcca gcgtcaggat catctctgcc gcatcaattg cggtgttcag
    11161 catcggctgg ccttttgccg gaccttccgc tttggtgtag ttgagcttac gcagcagatc
    11221 catctcgctc tgggtgttcc aggcaatccc tttaccgccg ttaccgattt tctccatcag
    11281 cgggccgata gaggtaaagc gttcgtaagt tgccggataa tcgcgctcta cgaccataat
    11341 gtgcggtgca gttttgcctg ggatcaggtc gcattcgcct tttttccagt ctttcacatc
    11401 cagcggctgc gccagttcag cggcagagtc gtgctggata ggcagcgtga cgatgtcggt
    11461 ttctttaccc agatagccga cgcacacttc ggagaatttc ttcgcgatgg ctttgtagat
    11521 ttcccagtcg cttttcgctt cccaggccgg atcgaccgcc gcagacagcg ggtgaataaa
    11581 cggatgcata tccgaagtat tcatgtcgtc tttttcgtac caggtcgccg tcggcaaaat
    11641 gatgtcggaa tagagacagg tgctcgacag acggaagtcc agcgtaacca ccagatccag
    11701 cttgccttcc agaccattgt cctgccagtc cacttcttcc ggcttcacgc cgccctgttg
    11761 ccccagatct ttaccctgga taccgtgctc cgtccccagc aggtacttga gcataaactc
    11821 atgaccttta ccggaagaac cgagcaggtt agaacgccag atgaacaggt tgcgcgggtg
    11881 gtttttaccg ttttctggtt gttctgccgc aaaacggatg gaaccctctt tcagggattt
    11941 caccgtatag tccaccggat tcatcccggc tttttccgct tcgccagcga tagtcagcgg
    12001 gttagtgcct aactgcggtg cagacggcag ccagcccatg cgttccgcac ggacgttaaa
    12061 gtcgatcaag tgtccggtat agcgggattt gtccgccatc ggtgacagca actcttccgc
    12121 cgtgacggtt tcataacgcc actggctgga gtggttatag aaataagaag tgctgttcat
    12181 gtgacgcgcc ggacgctgcc agtcaagggc aaacgccagc ggctgccagc cggtttgcgg
    12241 acgcagtttt tcctgaccta catagtgcgc ccagccgccc ccgctctgac cgacacagcc
    12301 gcagaaaatc agcatgttga tcagaccacg atagttcata tcgaggtgat accagtggtt
    12361 cagccccgca ccgacgataa tcatcgaacg accgtgcgtt ttatcagcgt tatcggcaaa
    12421 ttcacgggcg atgcgaataa tctggctgcg agaaacgccg gtaatctgct cggcccaggc
    12481 cggggtataa gctttcacat cgtcatagct ggttgcacag ttaacgtcgt tcaggccacg
    12541 ttccagaccg tagtttgcca gcgtcagatc ataaacggtg gtcaccaggg cggtgctgcc
    12601 atcagccagt tgcaggcgtt tcaccggcag tttgtgcagc agcacgtttt ccagttccac
    12661 tttgttgaag tgttccgtgc cgtcgccacc aaagtacggg aagcctacct cagcgatctc
    12721 atcctggcta cccagcaggc tcagttgcag ctcggtttct tcgccagttt tgccgtcgcg
    12781 ctgctcaaga ttccatttgc ccttctcgcc ccagcggaag ccaatagaac cgttcggcgc
    12841 aaccatttcg ccattggtat taaaggcgac agttttccat tccggattgt tttcctggcc
    12901 cagcgcatca accagatcag cagcgcgcag catacgacct gcagcgtagt aaccgtcgcg
    12961 ttcttccagc atcaccagca tcggcatgtc ggtgtagcga cgcacatagt cggtgaaata
    13021 ctggcttggg ttgtcgaggt ggaattcacg cagcattacg tggcccatcg ccagcgccat
    13081 tgccgcatcg gtgccctgtt tcggtgccag ccacagatcg cacagtttgg cgatttcagc
    13141 gtagtctggt gtgacggcaa cagttttggt gcctttgtaa cgcacttcag taaagaagtg
    13201 agcatccggg gtacgcgtct gcggcacgtt tgaaccccag gcgatgatgt agctggagtt
    13261 ataccagtca gcagattccg gtacgtcagt ttgctcgccc caggtttgcg gagacgcagg
    13321 aggcaggtcg cagtaccagt cgtagaagct taagcaagta ccgccaatca gcgagagata
    13381 gcgtgcaccc gatgcgtaag aaaccatcga cattgccgga attggcgaga aaccagcaac
    13441 acggtccggg ccgtagtttt tgatggtgta aacgttagat gcggcgatca gttcgttcac
    13501 ctcctgccaa gaagaacgaa caaatccacc gcgtccacgc gcctgcttaa agcttttcgc
    13561 tttatcggcg tcttcaatga tagaagccca tgcctcaacc ggatcgctat gcagcgcctt
    13621 cgcttcacgc cacattttca tcaggcgttt gcgcatcatc gggtatttca ggcggttggc
    13681 actgtaaaga taccaggagt agctggcacc gcgcgggcag ccgcgaggtt catggtttgg
    13741 cagatccgga cgggtacgcg gatagtcagt ctgctgggtt tcccaggtga ccagaccgtt
    13801 tttgacgtag attttccagc tgcaggagcc ggtgcagttt accccgtggg tagagcggac
    13861 gattttgtca tgctgccaac gctggcgata tccatcctcc cagtcacggt tggtattgag
    13921 aagctggcca tgcccatcgg caaaggtttc acccttctgt ttgaagtagc gaaaccggtc
    13981 caggaattta ctcatcggtt ttctcctgtg ggagcctgtc ggccctctga taaatcgaca
    14041 ttgctaatgt ttaagggcaa ggtaacgctc tgaaacagcg tgggaattga taacgatcaa
    14101 gagtgatggg gatgaaaaat aaagtaaatt cctaagatac ccctttagga taattaacag
    14161 ttacagcata acatgct
//
LOCUS       DAEBXN010000104        14093 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.104, whole genome shotgun sequence.
ACCESSION   DAEBXN010000104 DAEBXN010000000
VERSION     DAEBXN010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14093
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.104"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            663..1454
                     /locus_tag="KJL99_003243"
     CDS             663..1454
                     /locus_tag="KJL99_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4437861.1"
                     /translation="MTTRITFSMTSDETLRIVDEYCHTHKLSRSKVINALLSATAPVL
                     NDINCYYQLAGELQSRLLNGVYQRDLPRKRNVVSAEKYCLEIWENKLFTRRILEFDSS
                     NGVLYALKHKRHYRRDKMIGRVESRCIKDICEYQMQLSGEKTKYACFIYIERTIYNHD
                     NPSDKIPVKAAVGNAVILLAKDVIYNEYFFDLRQSFFVSVTDLMVSGAKGIPETQTYP
                     DVYCWIPLFSINSGVLITPVYKIDPLKPVTVKKPDKITVVCNYRE"
     gene            1840..2046
                     /locus_tag="KJL99_003244"
     CDS             1840..2046
                     /locus_tag="KJL99_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HBD4437862.1"
                     /translation="MNTPVSLMDDQMVDMSFITQLTGLTDKWFYKLIKHGAFPPPIKL
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            2132..2704
                     /locus_tag="KJL99_003245"
     CDS             2132..2704
                     /locus_tag="KJL99_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4437863.1"
                     /translation="MQQLENRCDLLLIQHQKWMTSVTRLIVAHGMGSPHLHGYHRLTL
                     AHFFLPEKGSVISVAPQGLYQVVNPGTPPFIPAIQEGLMTSIQTHEIMLLTHFNLGGV
                     LLSELHRLGENRLANRLNSLLRRFDDRDLYHTLIWLCWYDLMCAHSMQPWTEELKHKS
                     HAELENWAVARKREKRELELMIDEYLLYAC"
     gene            complement(2706..2990)
                     /locus_tag="KJL99_003246"
     CDS             complement(2706..2990)
                     /locus_tag="KJL99_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4437864.1"
                     /translation="MVVLNTPGKYQYDLRNHLLVNLFPIALDAEITTSNVNKHQLHRS
                     NIPAALSGNSGITDYVPFSAERNAAMVIWRTAIDSRPAVCNDEEGTPGTL"
     gene            2961..3104
                     /locus_tag="KJL99_003247"
                     /pseudo
     CDS             2961..3104
                     /locus_tag="KJL99_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            4273..5292
                     /locus_tag="KJL99_003248"
     CDS             4273..5292
                     /locus_tag="KJL99_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBD4437865.1"
                     /translation="MNNEQMLIKCRDPRKCRFYAEYLNHKYPNEFPHFAPDNPQNCIR
                     NVNLFLKEKYCNVSPPPFGTFGTTEEEALNNEINELEIIAGQAILPEESFLWLKTDEL
                     ATFFTWTTIYLSKEDHTKFPKINHNSSGIKNGIEVRKIRTVTDETLNTYKKSNLPDNP
                     SSHNERIKVIISYFDSCHSLINKNAKSIYLEELRQAWLKKKSACQKFKWLLPDDDEMC
                     QWFWTRLKQKQDEKHPVHNPVTRWFIPSSTKEYYLASLVAFALWKEAPDTIELFRTRI
                     NHAWHQKKQKDKYRSDGKKAINIHIRSNVKEMLDELRTVYGLRTGEFFEMLITEKYLE
                     HKRRK"
     gene            5402..6274
                     /locus_tag="KJL99_003249"
     CDS             5402..6274
                     /locus_tag="KJL99_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HBD4437866.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILQCGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQEQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRSQAREQFTGAVDRIFDTAESVCVASVVRTALRAVRDT
                     VVSVARAVWNWIFF"
     gene            6608..9730
                     /gene="ag43"
                     /locus_tag="KJL99_003250"
     CDS             6608..9730
                     /gene="ag43"
                     /locus_tag="KJL99_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HBD4437867.1"
                     /translation="MKRHLNTCYRLVWNHITGAFVVASELARAQGKRGGVAVALSLAA
                     VTSLPVLAADIVVHPGETVNGGTLVNHDNQFVSGTADGVTVSTGLELGPDSDENTGGQ
                     WIKAGGTGRNTTVTANGRQIVQAGGTASDTVIRDGGGQSLNGLAVNTTLDNRGEQWVH
                     GGGKAAGTIINQDGYQTIKHGGLATGTIVNTGAEGGPESENVSSGQMVGGTAESTTIN
                     KNGRQVIWSSGMARDTLIYAGGDQTVHGEAHNTRLEGGNQYVHNGGTATETLINRDGW
                     QVIKEGGTAAHTTINQKGKLQVNAGGKASDVTQNTGGALVTSTAATVTGTNRLGAFSV
                     VEGKADNVVLENGGRLDVLTGHTATNTRVDDGGTLDVRNGGTATTVSMGNGGVLLADS
                     GAAVSGTRSDGKAFSIGGGQADALMLEKGSSFTLNAGDTATDTTVNGGLFTARGGTLA
                     GTTTLNNGAILTLSGKTVNNDTLTIREGDALLQGGALTGNGSVEKSGSGTLTVSNTTL
                     TQKAVNLNEGTLTLNDSTVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLASGATWNI
                     PDNATVQSVVDDLSHAGQIHFTSTRTGKFVPATLKVKNLNGQNGTISLRVRPDMAQNN
                     ADRLVIDGGRATGKTILNLVNAGNSASGLATSGKGIQVVEAINGATTEEGAFIQGNKL
                     QAGAFNYSLNRDSDESWYLRSENAYRAEVPLYASMLTQAMDYDRILAGSRSHQTGVSG
                     ENNSVRLSIQGGHLGHDNNGGIARGATPESSGSYGFVRLEGDLLRTEVAGMSVTAGVY
                     GAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNLIHNASGLWADIVAQGTRHSMKASS
                     DNNDFRVRGWGWLGSLETGLPFSITDNLMLEPQLQYTWQGLSLDDGQDNASYVKFGHG
                     SAQHVRAGFRLGSHHDMNFGKGTSSRDTLRGSAKHSVRELPVNWWVQPSVIRTFSSRG
                     DMSMGTAAAGSNMTFSPSQNGTSLDLQAGLEARVRENITLGVQASYAHSINGSSAEGY
                     NSQATLNVTF"
     gene            9851..12367
                     /locus_tag="KJL99_003251"
     CDS             9851..12367
                     /locus_tag="KJL99_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4437868.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALTSTASALAGFILLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAAGLLALPVQIRTIQQNGLTPEDISKEINGYYCCFYTAFFLMACSAYAPLIALQFD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKRLTSAISQTLEEQPVLNSKSWLSSLQ
                     NDYSLPETLTERIWLTLISQRISRGELREFELADGNWLLNNAWYERNMAGFNEQLKEN
                     LSFTPDELKTLFRNRLNLSPEANDDFLDRCLDGGDWYPFSEGRRFVSFHHVDELRICA
                     SCGLTEVHHAPENHKPAPEWYCSSLCHETETLCQDIYERSYTGFISDATANGLILMKL
                     PETWSTNEKMFASGGQGHGFAAERGNHIVDRVRLKNARILGDNNARNGADRLVSGTEI
                     QTKYCSTAARSVGAAFDGQNGQYRYMGNHGPMQLEVPRDQYAGAVETMKNKIREGKVP
                     GVTDPAEASRLIRRGHLTYTQARNITRFGTIESVTYDIAEGSVVSLAAGGISFALTAS
                     VFWLSTGDRDAALQTAAVQAGKTFTRTLAVYVTTQQLHRLTVVQGMLKHIDFSTASPT
                     VRQALQKGTGAGNISALNKVMKGTLVTSLALVAVTTSPDMIKMLRGRISGAQFIRNLA
                     VASSGVAGGAVGSVAGGILFSPLGPFGALTGRVVGGVLGGMIASAVSGKIAGALVEED
                     RVKILAMIQEQVTWLAGSFLLTGHEIENLNANLARVIDQNALEIIFAAGIQQRAATNM
                     LIKPLVVSIIRQRPVMEYDASHLGKMVNRLEEAFPPELPA"
     gene            12443..12898
                     /locus_tag="KJL99_003252"
     CDS             12443..12898
                     /locus_tag="KJL99_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4437869.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNGEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDSNATRTTDAFLE
                     TECVENVATTEIIKATEESNGHRVSLPLSVFDPQDYHPLLITVSGKNVN"
     gene            complement(13017..13228)
                     /locus_tag="KJL99_003253"
                     /pseudo
     CDS             complement(13017..13228)
                     /locus_tag="KJL99_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386164.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            13309..>14093
                     /locus_tag="KJL99_003254"
     CDS             13309..>14093
                     /locus_tag="KJL99_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HBD4437870.1"
                     /translation="MQLVSRFGYANQIRRDRPLTHEELMHHVPGIFGEEKHTSRSQNY
                     TYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     TYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWV"
BASE COUNT         3830 a         3476 c         3461 g         3326 t
ORIGIN      
        1 ctcttgacta taaagatgat gacgggatca gaacagttat taatccatac aacaaaatac
       61 agaaaacaac agaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaataa cagacattac
      121 ttaacaaaga acattacagt cagtgtgtac attacagatt tatattcagg tgttgcggga
      181 ctacattaca gcagagaggg gtaaggacag agttacaagt actactagag agaatatcac
      241 tgcacagata cttctatgag tatatcttta tgagagatcc gtctacagga ttgggttata
      301 aggtgtattt acaaggtata atcacaggat catttacatg accatacttc tgtaaataat
      361 actaattaac cttaattatt tttttatggt acatgaacta cctgtagctg taacgataaa
      421 caaatcaccc aaaatgaaaa atactggtta tcacagttca gagaactcgt atgagtgtcc
      481 ccctgagcaa tgcttcattt tctgtgattc tgtgtgatag agttatactg agtacagtgg
      541 cagtaacact gctttccatc acttgaggat aaaattcatt aaaaaattaa ttgcatgaat
      601 taacaggttt ttttatgaaa tgttatgcat ctcaaccaat atgattaaca gaaaacttca
      661 tcatgacaac acgtataacg ttcagtatga ccagtgatga gacgctgcga attgtggatg
      721 agtactgtca tacccacaaa ctttcgcgct ctaaggtaat caacgcatta ctcagcgcca
      781 cagcacctgt ccttaatgat attaactgtt attatcaact tgcaggcgaa ctgcagtcac
      841 gcttattaaa tggagtgtat caacgggatc ttcctcgcaa acgcaatgtc gtcagtgcag
      901 agaagtattg tctggaaatc tgggaaaaca aacttttcac cagaagaatc ctggagtttg
      961 acagttcaaa tggtgttctg tacgcgctga aacataaaag acactatcgc cgggataaga
     1021 tgattggaag ggttgaaagt cggtgtatca aagacatttg cgaataccag atgcagctat
     1081 ccggggagaa gacgaaatat gcctgtttta tttatatcga aagaacaata tacaatcacg
     1141 ataatccatc tgataaaata cctgtaaaag cggctgttgg taatgcagtc atcctgctgg
     1201 caaaggatgt gatatataac gagtactttt ttgatttgcg gcagtcattt tttgtgagcg
     1261 ttacagatct gatggtctct ggcgcaaaag gtattccgga gacacagaca tatccggacg
     1321 tttattgctg gatccctctt ttttccataa attccggcgt gctcattacc cctgtatata
     1381 aaattgaccc tctgaaaccc gtaacggtaa aaaaaccgga taagatcact gttgtttgta
     1441 attacagaga ataatttcag ccttctacct tcaccgcgac agtgtttacc tctgctttat
     1501 cttccgttca gtaaatacaa accaccgctc tggcaggtac agggcgtggt ttatctccga
     1561 tactcccctg tgcagtaata atcatcactg aaaatactga tcccgtcacg gcaaaacgcc
     1621 atcccatatt ccggcaacac aaaatcatct tccgtttata ccggattcac tgacaaaaca
     1681 gacaacctgt attgcctgta cacaacagtt tctctcctgg caggaatatt cccctactca
     1741 ttttttacgt ttctccccgg ttgttatgcc ggtgaaggtg gtgcgtcgtt ttcatcacca
     1801 acgccggttg agattaacaa catccggagg aacattctca tgaatacccc agtttcactg
     1861 atggatgacc agatggtcga catgtcgttt atcactcagc tgaccggcct gaccgataag
     1921 tggttttata aactcatcaa gcatggagcc tttccgcccc ccatcaaact gggccgtagc
     1981 tcccgctggc tgaaaagcga agtggaagcc tggctgcagg cgcgtattgc acagtcccgt
     2041 ccgtaattcc tgaccgttat ccgttccacc gcagcaaagg catgatccgg cccccggagg
     2101 cattttgctg cgcgctaaag gagagaatat tatgcaacaa cttgaaaaca ggtgtgacct
     2161 gctgctcatc cagcatcaga aatggatgac aagcgtgacg aggcttatcg tcgctcatgg
     2221 aatggggtct ccgcatcttc atggttatca ccgtctgacc ctggcacact tttttctgcc
     2281 ggaaaaaggc tccgtcattt cggtggcccc ccagggactc tatcaggtcg tcaacccggg
     2341 aacaccaccg ttcattcctg ccattcagga agggttaatg accagcattc agacccatga
     2401 aatcatgctg cttacacatt ttaatctggg cggcgtttta ctcagtgagc tacatcgtct
     2461 gggtgaaaac cggctggcaa acaggctgaa cagcctgctc cggcgtttcg acgatcgtga
     2521 tctctaccac acgctgatat ggctgtgctg gtatgatctg atgtgtgccc actccatgca
     2581 gccctggact gaagagttaa agcataaatc acatgcagag cttgaaaact gggctgtggc
     2641 gcgcaaacgg gaaaagcgcg agctggaact gatgattgac gagtacctgt tatacgcctg
     2701 ctgattcata acgttcccgg ggttccttcc tcatcgttac acacagccgg acggctatct
     2761 atggctgtcc gccaaatgac catcgccgca tttcgttccg ctgaaaacgg tacgtagtca
     2821 gtgataccgg aatttccgga gagggccgca ggtatattgc ttctgtgcaa ttgatgctta
     2881 tttacattgg atgtagttat ttctgcatcc aatgctatcg gaaagagatt aaccagcaaa
     2941 tggttcctca aatcgtattg atatttaccc ggcgtattga gcaccaccat ctgacactcc
     3001 gttcccgcat caaacgactg gaccgaaaaa cgatttactt ttcacgtgct gtggaaattc
     3061 acgaaaaaat tattgggtca ttcatcgaaa aacatatgtt ctgatgactg gagtcatcct
     3121 ccagaaatat acgtcaactg aacaatatcg attttttatg ggattctata cacttttcca
     3181 tatccctctg aaaatcagtc atcagctcac caacacctct tctgaaactt gtattaaact
     3241 tattgcttaa atagatgata taacatctct tccattgcac tgaatcggcg aaaactgccg
     3301 tttcaataat ctcaacaata ttatttatcg gctaacttta tcagtaaaca atggaatgtc
     3361 acactgatga aatgaacatt cagagtaaga tgataaaatt catataaacg ccattgctga
     3421 atattattta tttcatacag ataaacgata aaatacaaaa gggtatattt ctcaatgtaa
     3481 cagtcaatta ttcatctcca ctggcgagca cggtcagcac acaaacaaag ttagtcctcc
     3541 tgcttaatca ttgcgtatca gaaactaaaa aagaaaaaat tctgcagaca acttaacaat
     3601 cttcatcaca tttcctgaag acagacatat ccacaaacgg tagtcagcgt tgaattaaca
     3661 agtcttacag agctatacgt agcttataaa gccactcaat gttcatcatc acccgactac
     3721 ttatctggaa taccaccagt ataaactcat caataaaaac acctgttgtt aaaataagaa
     3781 tactgtcagc agtaaattat taaaacaaca tctgtccaca ctatctgttc ttttaacgcc
     3841 aacggcagcg aaaatagtgc atcacagcct tcttttttct ttcgccacca ctcagacaaa
     3901 cacaacactc acacccacac cacaaaacag aatcaaaaca taaaattaac aataacagtc
     3961 aaaaggaaaa cacagaatat tgaggtggac tgtcaccagg acagcgcata cgacgcatgt
     4021 gatcagcaca tcccgttctt cgctggcatc cccaaaatgc agaacaaaca atcataacat
     4081 tatgaatata aaagaaaata tgatttttca ccatgaaaaa aacatcaatg tatctctgta
     4141 taaaaataat gataacatga cataaacatt taaaaacata aataaacagc aacaaaaaca
     4201 tttgataatc acttagtgat taaatacaat taagacgatt ttgttataag agaggcacca
     4261 tggagtgaga taatgaataa cgaacagatg cttatcaaat gcagagatcc ccgtaaatgt
     4321 cggttttatg ccgaatatct gaatcacaaa taccctaatg aattccctca ctttgcgcct
     4381 gacaacccac aaaattgtat caggaatgtt aatttatttt taaaggaaaa gtattgtaat
     4441 gtttcacctc ccccctttgg tacttttggt actactgaag aagaagcact caataacgaa
     4501 attaatgagc ttgaaattat tgccgggcag gcaatactgc ctgaagagag ttttctctgg
     4561 ttgaaaactg acgaacttgc gactttcttt acctggacta ctatttatct gtcgaaggaa
     4621 gaccatacaa aattcccgaa aataaaccat aattcatcag gaataaaaaa cggaattgaa
     4681 gtcaggaaaa tacgaacagt taccgatgaa acgttaaaca cttacaaaaa aagtaacctt
     4741 ccggataatc catcatccca taacgaacga ataaaagtaa tcatttcata ttttgactcc
     4801 tgccattctt taattaataa aaatgcaaaa agcatatacc tcgaggaact caggcaggcg
     4861 tggcttaaaa agaaaagcgc ttgtcaaaag tttaagtggc tccttcctga cgatgatgaa
     4921 atgtgtcagt ggttctggac aagactgaaa caaaaacagg atgaaaaaca tccagtacat
     4981 aacccagtga cccggtggtt cataccatct tcaacgaaag aatattatct tgcctctctt
     5041 gtcgcctttg ctttatggaa agaagcgcct gataccattg aactgttcag aacacgaata
     5101 aatcatgcct ggcatcagaa aaaacaaaag gacaaatatc ggagtgatgg taaaaaggcc
     5161 attaatatac atataagaag caatgtgaaa gaaatgcttg atgagctcag aactgtttac
     5221 ggtctgagaa caggagagtt ttttgaaatg ctgattacag agaaatatct tgaacacaag
     5281 cgaaggaagt aattcattac ctgttacatt ctaaataatc tcagacagat attatctccg
     5341 tgaatccccc gccacctttc cggtgcgcgg ggttttgtct tttttcaccg ggaatacatg
     5401 tatgaatccg tctgatgcca ttgaggcaat tgaaaaaccg ctctcctccc tgccttactc
     5461 actttcccgt cacatcctgg aacatctgcg caaactcacc agtcacgaac ccgtgattgg
     5521 cattatgggt aaaagcgggg ccggtaaatc ttcactctgt aatgcactgt ttcaggggga
     5581 ggtcacaccg gtcagtgatg ttcacgccgg cacccgggaa gtgcagcgct tccgtctgag
     5641 tggtcacggt cacagcatgg ttatcactga cctgcccggg gtgggcgaga gccgggacag
     5701 ggatgcagag tatgaagccc tgtaccgtga cattctgcct gaactggacc tggtgctgtg
     5761 gctgattaaa gccgatgacc gggccctgtc tgtggatgag tatttctggc gacacatcct
     5821 gcagtgcgga catcagcagg tgctgtttgt ggtgacgcag gctgacaaaa cggaaccctg
     5881 ccatgaatgg gatatggcag gtattcagcc ttctcccgca caggaacaga acattcgtga
     5941 gaaaacggag gcggtattcc gtctgttccg gcccgtacat ccggttgttg ccgtatcggc
     6001 ccgcaccggc tgggaactgg atacgctggt cagtgcgctc atgacagcgc ttcccgacca
     6061 tgccgccagc cccctgatga cccgactgca ggacgagctg cgcacggagt ctgtccgctc
     6121 tcaggcccgt gaacagttta ccggtgcggt ggaccggata tttgacacag cggagagcgt
     6181 ctgtgttgcc tctgttgtcc gtacggccct gcgcgctgtt cgtgacaccg tggtctctgt
     6241 tgcccgcgcg gtatggaact ggatcttctt ctgaacctgt tgtggatgat gtcctccctg
     6301 cctctgagtc tgctcacaaa agcgctgttt tcgttactgt ctctcttgtc cgtgcaatag
     6361 actgataata gaataaaacg atcgatacct attttaccga tcgttaatat cgatcgttat
     6421 gctaataata actcctgtta gcaacgtgcg cagatacaca cagacatgag attcagggaa
     6481 caacagagcc acacgtcaga aacttccgtc agccggacct ccggcactgt aaccctttac
     6541 ctgccggtat ccacatctgt ggataccggc ttttttattc accctcactc tgattaagga
     6601 aatgctgatg aaacgacatc tgaatacctg ctacaggctg gtatggaatc acattacggg
     6661 cgctttcgtg gttgcctccg aactggcccg cgcacagggt aaacgtggcg gtgtggcggt
     6721 tgcactgtct cttgccgcgg tcacgtcact cccggtgctg gctgctgaca tcgttgtgca
     6781 cccgggtgaa acagtgaatg gcggaacact ggtaaaccat gacaaccagt ttgtatccgg
     6841 aacagctgat ggcgtgactg tcagtaccgg gcttgagctg gggccggaca gtgacgaaaa
     6901 caccggcggg caatggataa aagcgggtgg cacaggcaga aacaccactg tcaccgcaaa
     6961 tggtcgtcag attgtgcagg caggaggaac tgccagtgat acggttattc gtgatggcgg
     7021 agggcagagc cttaacggac tggcggtgaa caccacgctg gataacagag gtgagcagtg
     7081 ggtacacggg ggagggaaag ctgccggtac aattattaac caggatggtt accagaccat
     7141 aaaacatggc ggactggcaa ccggaaccat cgtcaacacc ggtgcagaag gtggtccgga
     7201 gtctgaaaat gtgtccagcg gtcagatggt cggagggacg gctgaatcca ccaccatcaa
     7261 taaaaatggc cggcaggtta tctggtcttc ggggatggca cgggacaccc tcatttacgc
     7321 cggtggtgac cagacggtac acggagaggc acataacacc cgactggagg ggggtaacca
     7381 gtatgtacac aacggtggca cggcaacaga gacgctgata aaccgtgatg gctggcaggt
     7441 gattaaggaa ggaggaactg ccgcgcatac caccatcaac cagaaaggaa agctgcaggt
     7501 gaatgccggc ggtaaagcgt ctgatgtcac ccagaacacg ggcggtgcac tggttaccag
     7561 tacagctgca accgtcaccg gcacaaaccg cctgggagca ttctctgttg tggagggtaa
     7621 agctgataat gtcgtactgg aaaatggcgg acgcctggat gtgctgaccg gacacacagc
     7681 cactaatacc cgcgtggatg atggcggaac gctggatgtc cgcaacggtg gcaccgccac
     7741 caccgtatcc atgggaaatg gcggtgtact gctggccgat tccggtgccg ctgtcagtgg
     7801 tacccggagc gacggaaagg cattcagtat cggaggcggt caggcggatg ccctgatgct
     7861 ggaaaaaggc agttcattca cgctgaacgc cggtgatacg gccacggata ccacggtaaa
     7921 tggcggactg ttcaccgcca ggggcggcac actggcgggc accaccacgc tgaataacgg
     7981 cgccatactt accctttccg ggaagacggt gaacaacgat accctgacca tccgtgaagg
     8041 cgatgcactc ctgcagggag gcgctctcac cggtaacggc agcgtggaaa aatcaggaag
     8101 tggcacactc actgtcagca acaccacact cacccagaaa gccgtcaacc tgaatgaagg
     8161 cacgctgacg ctgaacgaca gtaccgtcac cacggatgtc attgctcagc gcggtacagc
     8221 cctgaagctg accggcagca ctgtgctgaa cggtgccatt gaccccacga atgtcactct
     8281 cgcctctggt gccacctgga atattcccga taacgccacg gtgcagtcgg tggtggatga
     8341 cctcagccat gccggacaga ttcatttcac ctccacccgc acagggaagt tcgtaccggc
     8401 aaccctgaaa gtgaaaaacc tgaacggaca gaatggcacc atcagcctgc gtgtacgccc
     8461 ggatatggca cagaacaatg ctgacagact ggtcattgac ggtggcaggg caaccggaaa
     8521 aaccatcctg aacctggtga acgccggcaa cagtgcgtcg gggctggcga ccagcggtaa
     8581 gggtattcag gtggtggaag ccattaacgg tgccaccacg gaggaagggg cctttatcca
     8641 ggggaataag ctgcaggccg gtgcctttaa ctactccctc aaccgggaca gtgatgagag
     8701 ctggtatctg cgcagtgaaa atgcttatcg tgcagaagtc cccctgtatg cctccatgct
     8761 gacacaggca atggactatg accggattct ggcaggctcc cgcagccatc agaccggtgt
     8821 aagcggtgaa aataacagcg tccgtctcag cattcagggc ggtcatctcg gtcacgataa
     8881 caacggcggt attgcccgtg gagccacgcc ggaaagcagc ggcagctatg gcttcgtccg
     8941 tctggagggt gacctgctca gaacagaggt tgccggtatg tctgtgaccg cgggggtata
     9001 tggtgctgct ggccattctt ccgttgatgt taaggatgat gacggctccc gtgccggcac
     9061 ggtccgggat gatgccggca gcctgggcgg atacctgaat ctgatacaca acgcctccgg
     9121 cctgtgggct gacattgtgg cccagggaac ccgccacagc atgaaagcct catcggacaa
     9181 taacgacttc cgcgtccggg gctggggctg gctgggttcg ctggaaaccg gtctgccctt
     9241 cagtatcact gacaatctga tgctggagcc gcaactgcag tacacctggc agggactctc
     9301 cctggatgac ggccaggata acgccagtta tgtgaagttc gggcatggca gtgcacaaca
     9361 tgtgcgtgcc ggcttccgtc tgggcagcca ccacgatatg aactttggta aaggcacctc
     9421 atcccgtgac accctgcgcg gcagtgccaa acacagtgtg cgtgaactgc cggtgaactg
     9481 gtgggtacag ccttctgtta tccgcacctt cagctcccgg ggggacatga gcatgggtac
     9541 agccgcagcc ggcagtaaca tgacgttctc accgtcacag aatggtacgt cactggacct
     9601 gcaggccgga ctggaagccc gtgtccggga aaatatcacc ctgggcgttc aggccagtta
     9661 tgcccacagc atcaacggca gcagcgctga aggttataac agtcaggcca cactgaatgt
     9721 aaccttctga taattctgca ttgcctctct gtggtcccgg tcatgatgac cgggacccgg
     9781 acgggggaaa acgtttcagt gccacattca ctggcattca caataacatg atattcatca
     9841 cggagtgact atgttacaga tagtcggcgc gctgattctg ctgatcgcag gatttgccat
     9901 tcttcgcctt ttgttcagag cattaaccag cacagcgtct gcgctggcag ggttcatatt
     9961 gctgtgtctg ttcggtccgg ctttactggc tggctatatc actgaacgca taacccggtt
    10021 attccatatt cgctggctgg caggcgtatt tctgacgatt gccggaatga tcatcagctt
    10081 catgtgggga cttgatggta aacatatcgc actggaggct catacttttg actctgtaaa
    10141 atttattctg accaccgctc tcgccgctgg tctgctggct cttcccgtgc agataagaac
    10201 cattcagcag aacgggctca cacctgaaga tatcagcaag gaaattaacg ggtattactg
    10261 ctgtttttat actgcttttt tccttatggc gtgttctgca tacgcaccat tgatcgcatt
    10321 gcagttcgat atttcaccct cactgatgtg gtggggcggg ttgttgtact ggctggctgc
    10381 attagtgacg ctgctatggg cggccagcca gatccaggcg ctgaaaagac tgaccagtgc
    10441 catcagccag acactggaag aacaaccggt gctcaacagt aaatcgtggc tgagcagttt
    10501 gcaaaacgat tacagccttc ctgaaacgct gacggagcgc atctggctga cactcatttc
    10561 acaacggatt tcccggggag aactgaggga atttgaactg gcagacggaa actggttact
    10621 gaacaatgcc tggtatgaaa gaaacatggc agggtttaac gaacagttga aagagaacct
    10681 gtcattcaca cctgatgaac tgaaaacgct cttccggaac cgcctgaatt tatcaccgga
    10741 agcgaatgac gattttctcg atcgttgcct ggacggcggt gactggtacc ccttttcaga
    10801 aggccgccgt tttgtatcat tccaccacgt ggatgagctt cgtatctgtg cctcctgcgg
    10861 gctgacagaa gtacatcatg ccccggaaaa tcataagccg gctccggaat ggtactgctc
    10921 ctctctttgt cacgaaacag aaacactgtg tcaggacatt tatgaacgtt cttacaccgg
    10981 ttttatttcc gatgcaacgg cgaatggtct gattctcatg aaactgccgg aaacctggag
    11041 tacaaatgag aaaatgtttg cttccggagg gcagggacat gggtttgccg ctgaacgggg
    11101 aaaccatatt gtcgacagag tccgtctgaa aaacgcacgg atcctcggtg ataataatgc
    11161 cagaaatgga gcagacagac tggtcagcgg aacagaaatc cagacgaaat attgttcaac
    11221 tgcagcccgt agcgtcggtg cggcattcga cggacaaaac ggacagtatc gttacatggg
    11281 aaatcatggt cccatgcaac tggaagtccc ccgtgatcag tatgccggcg ctgtggaaac
    11341 catgaagaat aagatccgcg aaggtaaagt acccggtgta accgatcccg cagaagcgtc
    11401 ccggctgatt cgtcggggac atctgactta tacccaggcc cgtaatatca cccggttcgg
    11461 gaccatcgaa tcggtcactt atgatattgc cgaggggtcg gttgtcagtc tggcggccgg
    11521 agggatcagt tttgccctga cggcatcggt cttctggctc agcaccggcg atcgcgatgc
    11581 tgccctgcag acagctgctg tccaggcagg aaaaaccttc acccgcacac tggctgtcta
    11641 cgtcacaacc cagcaacttc accggctcac tgttgttcag ggtatgctga agcatattga
    11701 tttttcgacg gccagcccga ctgtccggca ggcgcttcag aaggggaccg gtgcaggaaa
    11761 tatcagtgcc ctgaacaaag tgatgaaggg tacgctggtg acatctctgg cactggtagc
    11821 tgtcacaacc agccctgaca tgatcaaaat gttgcgggga cggatctccg gtgcgcagtt
    11881 catcaggaat cttgccgtgg catcttccgg tgtggcaggt ggtgctgtcg ggtcagtggc
    11941 gggcgggata ttgttcagtc cactgggacc atttggtgca ctgacagggc gtgtggttgg
    12001 cggtgttctg gggggaatga ttgcctccgc tgtatcagga aaaattgccg gagcgctggt
    12061 tgaagaagat cgcgtcaaaa ttctggcaat gattcaggag caggtgacat ggcttgccgg
    12121 cagtttcctg ctgaccggac atgagattga aaatctgaac gcgaatctgg cccgtgttat
    12181 cgatcagaat gctctggaga tcattttcgc cgccggtata caacaacggg ccgcgaccaa
    12241 tatgttaatc aaaccactgg tggtcagtat catcaggcaa cgccccgtca tggaatatga
    12301 tgcatcccat ctcggcaaga tggttaaccg gctggaagaa gcattccccc cggagctccc
    12361 ggcataacgt gtgttctgct ccttttaggt tttatcacct gatttgccag tgtcatcgtc
    12421 atgactcaga gaggtaaata ccatgattca cctgttcaaa acctgcatga ttaccgcctt
    12481 cattctgggg ttaacgtggt ctgccccact ccgggcacag gatcaacgtt acatcagtat
    12541 acgcaataca gatacgatat ggctcccggg aaatatttgt gcttaccagt tccggctgga
    12601 taatggcgga aacggtgaag gatttggccc cctcaccatc actctgcaac tcaaagacaa
    12661 atatggtcag acgctggtga ccagaaaaat ggaaacggaa gcttttggtg acagtaatgc
    12721 cacgcgaacc acagacgcat ttctggaaac ggagtgcgtg gaaaatgtcg ccacaaccga
    12781 aatcattaaa gcaactgaag aaagtaacgg ccatcgtgtc agtctgccgt tatcggtttt
    12841 cgatccccag gactaccatc cactgctgat taccgtttcc ggaaaaaatg ttaactgatc
    12901 cctgaagtca gtttcatgct tcagcctctg ccacaacgta tggcagaggc tatttatcag
    12961 gagaaaccag atatgccggg ttacacacag agtatgtcct ggctgaagga tctttctcat
    13021 acgggcaggc ggttgctgtc ataaccgctt accgcaatgt gtttatccag gatgacccgg
    13081 gaatgcattt ccgtcgggtt atccgcaacg cggaaggtca gcgacgctga cgcagccgga
    13141 actctgaagc tgatgccgga aaacagctta atgcgtggct cgccagtgga gggcttctca
    13201 gacaataaac ttcattgatt tcatccatca ggccgcgtct tctccgggag acgcggcttt
    13261 tttcatttgt tgcaccagta aatcttaacc accgataagg agcaaagtat gcaattagtc
    13321 agtcgttttg gttatgcgaa ccagatacgc cgtgaccgtc cgctgacaca tgaagagctg
    13381 atgcaccatg tgccggggat tttcggggaa gagaaacaca cgtcccgaag ccagaactat
    13441 acgtacatcc ccaccatcac cgtactggaa agtctgcagc gggaaggctt ccagcccttt
    13501 ttcgcctgcc agacccgtgt gcgcgacccg ggccgccggg gatacacaaa acacatgctg
    13561 cgtctgcggc gggccggaga gataaacgga gaacatgtcc ctgaaattat tctgctcaac
    13621 tcccatgacg gtacctccag ctaccagatg ctgccgggtt acttcagatt cgtctgccag
    13681 aacgggtgtg tctgtggtca gtctctgggg gaagtgcgtg ttccgcaccg gggaaacgtg
    13741 gtggagaaag ttattgaagg ggcttacgaa gtggtgggcg tgtttgaccg gattgaggag
    13801 aaacgtgatg ccatgcagtc gctggtcctg ccgccaccgg cacgccaggc gctggcacag
    13861 gcggcactga cttaccgtta tggtgacgaa catcagcccg tcaccaccgc cgacattctg
    13921 acgccacgac gccgggagga ttacggtaag gacctgtgga gtacttatca gaccatccag
    13981 gagaatatgc tgaaaggcgg gatttccggc cgcagtgcaa aaggaaaacg tatccacacc
    14041 cgtgccattc acaacatcga caccgatatt aagctcaacc gcgcattgtg ggt
//
LOCUS       DAEBXN010000105        14068 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.105, whole genome shotgun sequence.
ACCESSION   DAEBXN010000105 DAEBXN010000000
VERSION     DAEBXN010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14068
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.105"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..290
                     /locus_tag="KJL99_003255"
     CDS             <1..290
                     /locus_tag="KJL99_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="type IV secretion system coupling protein; similar
                     to F plasmid TraD; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HBD4437871.1"
                     /translation="QQPVSSVINDKKSDAGVSVPAGGIEQELKMKPEEEMEQQLPPGI
                     SESGEVVDMAAYEAWQQENHPDIQQHMQRREEVNINVHRERGEDVEPGDDF"
     gene            290..5560
                     /gene="traI"
                     /locus_tag="KJL99_003256"
     CDS             290..5560
                     /gene="traI"
                     /locus_tag="KJL99_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HBD4437872.1"
                     /translation="MLSFSVVKSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQDPQLHTHV
                     VVANVTQHNGEWKTLSSDKVGKTGFSENVLANRIAFGKIYQSELRQRVEALGYETEVV
                     GKHGMWEMPGVPVEAFSSRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEIRMAE
                     WMQTLKETGFDIRAYRDAADQRAEIRTQAPGPASQDGPDVQQAVTQAIAGLSERKVQF
                     TYTDVLARTVGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSV
                     RALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVD
                     QGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQR
                     PATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVL
                     GHPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLT
                     LRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASV
                     SEDAMTVVVPGRAEPATLPVADSPFTALKLENGWVETPGHSVSDSATVFASVTQMAMD
                     NATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETLLETAISLQ
                     KAGLHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTGFTELGGEINAQIKR
                     GDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMETLTSG
                     QRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAV
                     GEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIA
                     AGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQTPELREAVYSLINR
                     DVERALSGLESVKPSQVPRLEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDI
                     PMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVMVP
                     VLNTANIRDGELRRLSTWEKNPDALALVDSVYHRIAGISKDDGLITLQDAEGNTRLIS
                     PREAVAEGVTLYTPDKIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQ
                     QTRVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKQMAGFESAYVAL
                     SRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVLEPKPDREVMNAQRLFSTARELRDV
                     AAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGN
                     GLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
                     GRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEI
                     VRKMAENKPDLPDGKTEQAVREIAGQERDRTATSEREAALPESVLRESQREREAVREV
                     ARENLLQERLQQMERDMVRDLQKEKTLGGD"
     gene            5580..6326
                     /gene="traX"
                     /locus_tag="KJL99_003257"
     CDS             5580..6326
                     /gene="traX"
                     /locus_tag="KJL99_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HBD4437873.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSHRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVSC
                     AGKSLPRFWPGDFFPVFYACHLAVLGVLAL"
     gene            6381..6941
                     /locus_tag="KJL99_003258"
     CDS             6381..6941
                     /locus_tag="KJL99_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HBD4437874.1"
                     /translation="MTEQKRPVLTLKRKTEGETPVRRRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPSLDEAVNTLKPWWPGLFDGETPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAARLDKIRRQNRIKAELQAVLDEK"
     gene            7073..7285
                     /locus_tag="KJL99_003259"
     CDS             7073..7285
                     /locus_tag="KJL99_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HBD4437875.1"
                     /translation="MNGFRNSSRNGQVWRWQRAGSRAVILEVSGRWMEAAEAWRRAAG
                     VAPRTDWQQFARKRAEHCHRRSRGRV"
     gene            7531..7992
                     /locus_tag="KJL99_003260"
     CDS             7531..7992
                     /locus_tag="KJL99_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HBD4437876.1"
                     /translation="MRKYIPLVLFIFSWPVLCADIHGRVVRVLDGDTIEVMDSRKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVTYFQRDRYGRILGQVYAPDGMNVN
                     QFMVRAGAAWVYEQYNTDPVLPVLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            8038..8247
                     /gene="hha"
                     /locus_tag="KJL99_003261"
     CDS             8038..8247
                     /gene="hha"
                     /locus_tag="KJL99_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HBD4437877.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLLNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            8285..8875
                     /locus_tag="KJL99_003262"
     CDS             8285..8875
                     /locus_tag="KJL99_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HBD4437878.1"
                     /translation="MKLIIFILIVLIIAALLIRIILKSVNQHSPLLMQLHAAGIRTGD
                     AERILSGGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVERRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            9030..9503
                     /locus_tag="KJL99_003263"
     CDS             9030..9503
                     /locus_tag="KJL99_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4437879.1"
                     /translation="MKYDDVSELMIKREHNILIVGNSASHIDKFFKGYSIPGSKYYDF
                     TQINSDSDVERNEDAVSFIRDAMNSEEQTIIFNCAGWPDLGGGSAVSQFAMMVRKFGK
                     QLIVAVSEKDAIKLKDNFDIIGMLSYGKENFIAMSHTKNELTGGKRRYRIKNGCK"
     gene            9750..9899
                     /locus_tag="KJL99_003264"
     CDS             9750..9899
                     /locus_tag="KJL99_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HBD4437880.1"
                     /translation="MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLA
                     YEARK"
     gene            10184..10432
                     /gene="repA"
                     /locus_tag="KJL99_003265"
     CDS             10184..10432
                     /gene="repA"
                     /locus_tag="KJL99_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HBD4437881.1"
                     /translation="MSQTENAVTSSSGTKRAYRKGNPLTLAERQQASLARKRATHKEL
                     RVFIPAALKAQLQVMCEAEGVTQAEMIAELIKQKSAFS"
     gene            10677..10751
                     /gene="tap"
                     /locus_tag="KJL99_003266"
     CDS             10677..10751
                     /gene="tap"
                     /locus_tag="KJL99_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HBD4437882.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            10744..11601
                     /gene="repA"
                     /locus_tag="KJL99_003267"
     CDS             10744..11601
                     /gene="repA"
                     /locus_tag="KJL99_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HBD4437883.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPRKGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAAAAARRSRVVWENKQRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSRGIK
                     RARARRDADRERQDIVTLVKRQLTREIAEGRFTANREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            12302..12442
                     /locus_tag="KJL99_003268"
     CDS             12302..12442
                     /locus_tag="KJL99_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4437884.1"
                     /translation="MAESGYGLAGLGMGKVKSINQYRLTPGFGGFTPVSYETTECRLP
                     CR"
     gene            12540..13193
                     /locus_tag="KJL99_003269"
     CDS             12540..13193
                     /locus_tag="KJL99_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001757010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBD4437885.1"
                     /translation="MIQTRNQYLQFMLVMLAAWGISWGARFVMEQAVLLYGSGKNYLF
                     FSHGTVLMYLLCVFLVYRRWIAPLPVVGQLRNVGVPWLVGAMAVVYVGVFLLGKALAL
                     PAEPFMTKLFADKSIPDVILTLLTIFILAPLNEETLFRGIMLNVFRSRYCWTMWLGAL
                     ITSLLFVAAHSQYQNLLTLAELFLVGLITSVARIRSGGLLLPVLLHMEATTLGLLFG"
     gene            13286..13543
                     /gene="pemI"
                     /locus_tag="KJL99_003270"
     CDS             13286..13543
                     /gene="pemI"
                     /locus_tag="KJL99_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PemI"
                     /protein_id="HBD4437886.1"
                     /translation="MHTTRLKRVGGSVMLTVPPALLNALSLGTDNEVGMVIDNGRLIV
                     EPYRRPQYSLAELLAQCDPNAEISAEEREWLDAPATGQEEI"
     gene            13476..13877
                     /gene="pemK"
                     /locus_tag="KJL99_003271"
     CDS             13476..13877
                     /gene="pemK"
                     /locus_tag="KJL99_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001398199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin
                     endoribonuclease PemK"
                     /protein_id="HBD4437887.1"
                     /translation="MLKYQLKNENGWMHRRLVRRKSDMERGEIWLVSLDPTAGHEQQG
                     TRPVLIVTPAAFNRVTRLPVVVPVTSGGNFARTAGFAVSLDGVGIRTTGVVRCDQPRT
                     IDMKARGGKRLERVPETIMNEVLGRLSTILT"
BASE COUNT         3404 a         3360 c         4226 g         3078 t
ORIGIN      
        1 cacaacagcc agtgtcttct gtcatcaatg ataagaagtc agatgcaggt gtgagtgttc
       61 cggcaggggg gatcgagcag gagctgaaaa tgaaaccgga agaagagatg gaacagcaac
      121 tgccacccgg gatcagtgaa tccggtgaag tggtggatat ggccgcttat gaggcatggc
      181 aacaggaaaa tcatccggac atccagcagc atatgcagcg tcgtgaagag gtgaacatta
      241 atgtgcaccg ggagcgcggg gaggatgttg agccgggaga tgatttctga tgttgagttt
      301 cagcgttgtt aaatcggcag gcagtgccgg aaactattac accgacaagg ataattacta
      361 tgtgctgggc agcatgggag aacgctgggc cggtcagggg gctgaacagc ttgggctgca
      421 gggcagcgtc gataaggatg tttttacccg tcttctggag ggcaggctgc cggacggggc
      481 ggatttaagc cgcatgcagg atggcagtaa taagcatcgt cccggctacg acctgacctt
      541 ctccgccccc aaaagtgtct ccatgatggc catgctcggg ggggataaac gtcttattga
      601 tgcacataac caggccgtgg attttgctgt ccgtcaggtg gaggcgctgg cttccacacg
      661 ggtgatgacg gacggacagt cagaaacggt gctgaccggt aatctggtga tggcactgtt
      721 taaccatgac accagtcgcg atcaggaccc acagttacac acgcatgtgg tggtggctaa
      781 tgtcacgcag cataacggcg agtggaagac actgagcagt gacaaagtgg ggaaaaccgg
      841 attttccgag aacgtgctcg cgaaccggat tgcgttcggg aaaatatatc agagtgagtt
      901 acgtcagcgc gttgaggcgc tgggttatga aaccgaagtg gtcggtaagc acggtatgtg
      961 ggagatgccg ggcgtaccgg tggaggcctt ttccagtcgt tcacaggcta tcagggaggc
     1021 cgtcggtgaa gacgcctcgc tgaaatcccg ggatgtggcg gcactggata cgcgaaaatc
     1081 caaacagcac gtcgatccgg aaatcagaat ggctgaatgg atgcagacgc tgaaggaaac
     1141 cgggttcgac atccgggcat atcgtgacgc ggcggatcag cgtgcggaga tccgcacgca
     1201 ggcgcccggg cctgcttcac aggacgggcc ggatgtgcag caggcggtga cacaggcgat
     1261 tgccggatta agtgaacgaa aagtgcagtt cacgtatacg gacgtgctgg ccaggacggt
     1321 cggcatactg ccgccggaaa atggtgtgat tgaacgggca cgcgccggta tcgatgaggc
     1381 catcagccgt gagcagctta tccccctcga ccgtgagaag gggctgttca catcaggaat
     1441 tcatgtgctc gatgagctgt ccgtccgggc actcagtcgt gacatcatga aacagaaccg
     1501 ggtgaccgta catccggaga aaagtgtccc ccggacggct ggttacagcg atgcggtcag
     1561 tgttctggca caggaccgtc cgtcgctggc cattgtgtcc gggcagggcg gtgcagccgg
     1621 gcagcgtgag cgggtggctg aactggtcat gatggcccgg gaacaggggc gggaggtgca
     1681 gattatcgct gctgaccgtc gctcgcagat gaacctgaag caggatgaac ggctgtccgg
     1741 tgaactgata accggacgtc gtcagctgct ggaaggcatg gccttcacgc cgggcagtac
     1801 cgttatcgtc gaccagggtg aaaaactctc cctgaaagag acgttaaccc tgctggatgg
     1861 tgccgcacgt cataacgtac aggtcctgat aaccgacagc gggcagcgaa ccggtacagg
     1921 cagtgcgctg atggccatga aggatgccgg ggtgaacaca tatcgctggc agggcggaga
     1981 acagcgaccg gccaccatca tcagtgaacc ggaccgtaat gtccgctatg cccggctggc
     2041 cggagatttt gcggccagcg tgaaagccgg agaagagagc gtggcacagg tcagcggggt
     2101 acgggaacag gccatactga cacaggccat tcgcagtgag ctgaaaacgc agggcgtgct
     2161 cggacaccct gaggtgacca tgactgcact gtcaccggtc tggctggaca gccggagccg
     2221 ttatctgcgg gatatgtacc gaccggggat ggtgatggag cagtggaacc cggagacacg
     2281 cagtcatgac cgctatgtta tcgaccgggt gacggcgcag agtcacagcc tgaccctgcg
     2341 ggatgcgcag ggcgaaacgc aggtggtgcg tatttcctcc ctggacagca gctggtcgct
     2401 gttccggccg gaaaaaatgc cggtggcaga cggcgagcga ctgagggtga cagggaaaat
     2461 tcccggactc cgcgtctccg gcggtgaccg cctgcaggtg gcatccgtca gtgaagatgc
     2521 gatgacggtt gttgtgccgg ggcgggctga accggccacc ctgcctgtgg ctgattcacc
     2581 gttcacggca ctgaagctgg agaacggctg ggtggaaacg cccgggcatt ccgtcagtga
     2641 cagtgcgacg gtgtttgcct ccgtcacaca gatggcaatg gacaacgcca ccctgaacgg
     2701 tctggcccgc agcggtcgtg atgtccggct gtattcctca ctggatgaaa cccgtactgc
     2761 ggaaaaactc gcccgccatc cgtcctttac ggtggtttct gagcagataa aggcgcgtgc
     2821 cggtgagaca ttgctggaaa ccgctatcag tctgcagaaa gccgggcttc acacgccggc
     2881 acagcaggcc attcatctgg cccttcctgt gctggaaagt aaaaacttgg ccttcagcat
     2941 ggtggacctg ctgacagagg cgaagtcgtt tgctgcagaa ggaaccggtt ttactgaact
     3001 gggaggggaa atcaatgcgc agataaaacg cggtgactta ctgtatgtgg atgtggcaaa
     3061 aggctatggc acaggcctgc tggtttcccg tgcgtcgtat gaggcagaaa agagcattct
     3121 tcgccatatt ctcgaaggca aggaggcggt cacgccgctg atggagagag tacccggcga
     3181 actcatggag acgttaacgt cgggacagcg tgccgccacc cgtatgatac tggaaacgtc
     3241 cgaccgtttc acggtggtac aggggtatgc cggtgtgggt aagaccactc agttccgggc
     3301 ggtgatgtca gccgtgaaca tgctgccgga gagtgagcgt ccccgggttg tggggctggg
     3361 gcccacgcac cgtgcggtcg gtgagatgcg cagcgccggc gtggatgcac agacactggc
     3421 gtcctttctg catgacacgc agctgcagca gcgcagcgga gaaacgccgg atttcagcaa
     3481 cacgctgttt ctgctcgatg agagctcaat ggtgggcaat accgacatgg cacgggcata
     3541 cgccctgatt gcggccggtg gcggtcgtgc tgtggccagc ggtgacacgg accagctgca
     3601 ggccatcgcg cccggtcagc ctttccgtct ccagcagacg cgcagtgctg ccgatgtggc
     3661 catcatgaag gagattgtgc gtcagacgcc ggaactgcgg gaggcggtat acagcctgat
     3721 taaccgggat gtggaaaggg cactgtccgg gcttgagagt gtgaaaccgt ctcaggtgcc
     3781 acgtctggag ggcgcatggg caccggagca ctccgtgacg gagttcagtc acagccagga
     3841 agcgaaactg gcagaagcgc agcagaaggc gatgctgaaa ggcgaggctt tcccggatat
     3901 ccccatgaca ctgtatgagg ccattgtccg cgactatacc ggcaggacac cggaagcacg
     3961 ggagcagacg ctgattgtca cgcacctgaa tgaggaccgg cgcgtactga acagcatgat
     4021 tcatgatgca cgggaaaagg ccggtgagct gggcaaagag caggtcatgg tgcctgtcct
     4081 gaacacagcg aatatacgtg acggagaact gcgtcgtctc tccacctggg agaaaaaccc
     4141 ggatgccctt gccctggtgg atagtgtgta tcaccggatt gccggtatca gcaaggatga
     4201 cgggctgata accctgcagg atgcggaagg taacacgcgg ctgatttcgc cccgggaggc
     4261 ggtggctgaa ggtgtcacac tgtacacccc ggacaaaatc cgggtgggga ccggcgaccg
     4321 gatgcgcttc acgaagagtg accgggagcg cggttatgtg gccaacagcg tctggacggt
     4381 gacagcggtt tccggtgaca gtgtcacgct gtcggacgga cagcagaccc gggtgattcg
     4441 ccccggtcag gagcgggcag agcaacatat tgacctggcc tatgccatca ctgcccacgg
     4501 tgcgcagggg gcgagtgaaa cctttgccat cgcgcttgaa ggcacggaag gtaaccggaa
     4561 acagatggcc ggctttgagt cagcctacgt ggccctgtcg cgtatgaagc agcatgtgca
     4621 ggtgtacacc gataaccgtc agggctggac ggatgccatt aacaatgccg tacagaaagg
     4681 aactgcccac gatgtgctgg agccgaaacc ggaccgggag gtcatgaatg cgcagcggct
     4741 gttcagtacg gcacgggaac tgcgggacgt ggcggcaggc cgtgccgttc tccgtcaggc
     4801 ggggctggcc gggggagaca gtccggcacg gtttattgca ccggggcgca aatatccgca
     4861 gccgtatgtg gcactgcctg cttttgaccg taacggcaag tccgccggta tctggctgaa
     4921 cccgctgacc acggatgacg gaaacgggct gcggggattc agcggtgaag gacgggtgaa
     4981 aggcagcggg gatgcgcagt ttgtggccct gcagggcagc cgtaacggcg agagcctgct
     5041 ggctgataat atgcaggatg gtgtccggat tgcccgtgat aaccccgaca gcggtgtggt
     5101 ggtgagaatc gccggtgaag gtcgtccgtg gaatcccggt gccatcaccg gtggtcgcgt
     5161 gtggggggat atcccggaca acagcgttca gccgggagcc ggaaatggcg aaccggtcac
     5221 ggcagaggta ctggcacagc ggcaggctga agaggccatc cgccgtgaaa cggaacgtcg
     5281 cgcagatgaa attgtccgta aaatggcaga gaacaaacct gacctgccgg acggcaaaac
     5341 agagcaggct gtcagggaga ttgccggaca ggagcgtgac cggactgcca cttctgaacg
     5401 ggaagccgcg ctgccggaga gtgtgctgcg tgaatcacaa cgggagcggg aagcggtccg
     5461 ggaggttgcc cgggaaaatc tgctgcagga gcgtctgcag cagatggagc gggatatggt
     5521 tcgtgacctg cagaaagaga aaaccctggg cggagactga tacaggaaga taaacgctga
     5581 tgacaacgga taacacgaac acgacacgta acgattcact ggctgcacgg accgatacct
     5641 ggttgcagtc atttctggtc tggtcacccg gacagcggga tatcatcaaa acggtggcac
     5701 tggtgctgat ggtactggac cacatcaatc tgatattcca gctgaagcag gaatggatgt
     5761 ttctggccgg gcgaggagcc tttccgctgt ttgccctggt gtgggggctg aatctgtccc
     5821 gtcatgcgca tatccggcaa ccggccatta accggctgtg gggatggggg attattgccc
     5881 agttcgcata ttatctggca ggctttccct ggtatgaggg gaatatcctg tttgcctttg
     5941 cagtggcggc acaggtgctg acgtggtgtg aaacgcgcag tgggtggcgt acagcagcgg
     6001 ccattcttct gatggccctg tgggggcctt tgtccggcac cagttacggc attgccgggc
     6061 tgctgatgct ggcagtcagc caccggctgt accgggcgga agacagagcg gaacgtctgg
     6121 cgctggtcgc ctgtctgctg gctgttattc cggcgcttaa ccttgccacc agtgatgcag
     6181 cggcggtggc tggtcttgtg atgacggtgc tgaccgttgg tctggtgtcg tgtgcaggaa
     6241 aatcattacc ccgtttctgg cccggggatt ttttcccggt attctacgcc tgtcatctgg
     6301 ctgtgctggg cgttctggcg ctgtgacggg tgtggtatct ttgaccgtaa caggatgatt
     6361 cgtcagaggc agaacacagc atgacagagc agaagcgacc ggtactgaca ctgaagcgaa
     6421 aaacggaagg ggagacgcct gtccgcaggc ggaaaaccat catcaatgtc accacgccac
     6481 caaagtggaa ggtgaaaaag cagaagctgg ccgagaaagc agcccgggaa gcggagctgg
     6541 cggcaaaaaa agcgcaggcc agacaggcgc tgtccattta tctgaacctg ccctcgctgg
     6601 atgaggccgt gaacaccctg aaaccctggt ggccgggatt atttgacggt gaaacgcccc
     6661 ggcttctggc ctgcggtatc cgggacgtgt tactggaaga cgtggcgcag cggaatatcc
     6721 cgctctcgca taaaaaactg cgcagggcgc tgaaggccat cacccgttca gaaagctatc
     6781 tgtgtgccat gaaagccggt gcctgccggt atgatacgga agggtatgtg acggagcata
     6841 tttctcagga ggaggaagcg tatgcggcag cgcgtctgga taaaatccgc cgccagaaca
     6901 ggataaaggc agagcttcag gccgtgcttg atgagaaata aaaaaaagcc tccatcagag
     6961 aggaggcagg gaaataaggc ttaaaaggaa ataacttccg tacccgaaag gaagccaatt
     7021 cagtagatta tttaacacca taatactgac gggttaaaga ggagtgataa agatgaatgg
     7081 attcagaaac agttcacgga acggtcaggt ctggcgctgg cagcgtgccg gaagccgggc
     7141 cgttattctg gaggtcagtg gacgctggat ggaagcggca gaagcatggc gacgggctgc
     7201 cggtgttgcg ccccggacag actggcagca gtttgcccga aaaagggctg aacactgcca
     7261 ccggcgcagc cggggaaggg tgtaacgaaa aaagcagcct tcattactcc agggattcag
     7321 aaggctgaat aatgcatgag atttgctttt tttaatatcc ggtcgggcag tatgaccgga
     7381 cagttctgag tagcacagag ttttttccgt attaagtcgt gattttatat tttgtgatta
     7441 atttcacaaa ataaggtgtt attcagtgtg tgctgcgata ttcaggatta cctgaaatac
     7501 aggtgccatt tatctgatat ggagaataac atgaggaaat atattccact ggtattattt
     7561 atcttttcat ggccggtatt gtgtgcagat attcatggac gggttgttcg tgtactggac
     7621 ggtgatacca tcgaggtcat ggactcacgg aaggcagtgc ggatcaggct ggtaaatatt
     7681 gatgcaccgg aaaagaaaca ggattatggc cggtggtcaa cggacatgat gaaatcactg
     7741 gttgcaggaa aaacggttac cgtcacatat tttcagcgag atcgttatgg tcgtattctc
     7801 gggcaggttt atgcaccaga cggaatgaat gttaatcagt ttatggtccg ggccggggct
     7861 gcctgggtat atgagcagta caacacagat cccgtgctgc ctgtccttca gaacgaagcc
     7921 agacaacaaa aacgtgggct ctggtcagat gctgatccgg ttcccccctg gatatggaga
     7981 caccgtaaat aaagcgatgg caatcttatc attcatctgt tctcagcggg aataaaaatg
     8041 gcgaaaacaa aacaggaatg gctgtatcag ttacggcgat gttcatcagt caatacgctg
     8101 gaaaaaatta ttcataaaaa cagagacagt ttattaaaca gtgaacgtga atcatttaat
     8161 tcagcagcag accaccggtt agcggaatta atcaccggaa agttatatga caggatacca
     8221 aaagagatat ggaaatatgt aaggtaataa ttcagaataa ttaattacca ggagcagata
     8281 aacaatgaag ctgattattt ttattctcat tgttctgatt attgcagcgt tacttatccg
     8341 gattatcctc aaaagtgtaa atcagcattc tcctttgctg atgcagttgc atgcggcagg
     8401 tatacggacc ggtgatgcag agcgaatact gtccggcggt gaatactggc aacgtcagaa
     8461 gacgctgctg acagaaaggg aagtcagttt tatgaaagga ctgttcagaa ttgtggatat
     8521 gaagcggtgg tatctgtgtc cgcaggtacg ggtcgcggat atcgtccagc tgaacgggaa
     8581 tatccggcca cgatcacgcc agtggtggca gttattcagg atggtgtctc agtggcatgt
     8641 tgatgtggtt atcgttgaac ggcgttcgtt cagtattgtt gcagcagtag agctggatga
     8701 tgccagccat ttacgaccgg aacgccggcg ccgggatatt cttctggaag aggttctgag
     8761 gcaggctggt attccgttgc tcagaagcca cgatgccaga aaactgctgc agatgaccgg
     8821 agaatggctg aatacaacag gggctgatca gcagtccccg gaacatcgta gctgacgcct
     8881 tcgcgttgct cagttgtcca accccggaaa cgggaaaaag caagttttcc ccgctcccgg
     8941 cgtttctgta ataaaaagcg ggaaatggtg gtcacagagt aaattccgga accacctgta
     9001 cccaaacttt atataataat ggtgaatata tgaaatatga tgatgtatca gaattgatga
     9061 ttaagcgtga acacaatatt cttattgttg ggaactctgc ctcccacatt gataagtttt
     9121 ttaaggggta ttctatacca ggttctaaat attatgattt cactcagatt aacagtgatt
     9181 cagatgtaga gagaaatgag gatgctgtct cctttatcag ggatgcaatg aatagtgaag
     9241 agcagacaat catttttaat tgtgctgggt ggcctgattt aggtggtgga tcagcagtat
     9301 cacagtttgc aatgatggtc agaaaatttg gtaaacaact tattgtcgct gtaagtgaaa
     9361 aggatgccat caaattaaaa gacaactttg atattattgg gatgttgtca tatggtaaag
     9421 aaaattttat tgccatgagt catacgaaaa acgagttaac tggaggaaaa cgtcggtacc
     9481 ggattaaaaa tggatgtaaa taaaaagcat acttattggc tggagtgacg ccttcgtgct
     9541 tctgtagtaa aaagcgggca atgcaggtaa tagtcagcaa cagggaatgt ggtattatcg
     9601 cggcgggtgt ctgagccttt ctggttcagg caagacgcag gtaccagaaa tgcaaagacc
     9661 ccacttgtta atccattaac tcgtgaggtc tgcatgaagt acattgcact actgattgta
     9721 gcctcttcct tgcagagagg tcaaagttta tgacgaagta tgcccttatc gggttgctcg
     9781 ccgtgtgcgc cacggtgttg tgtttttcac tgatattcag ggaacggtta tgtgagctga
     9841 atattcacag gggaaataca gtggtgcagg taactctggc ctacgaagca cggaagtaaa
     9901 ctgccgggcg gggacggaag tccccgcttt ccggaagtgt gaggtatttc aggggcagac
     9961 acccgacatg ccagaaacag ccggtcccgc ccggggccgg ctcccgggtt aaggcatttc
    10021 ctgttttttc agccatttcg caacaaaaat cacattaaac aacagtaatc cccgttgatt
    10081 tgtgcgccaa cacagatctt cgtcacaatt ctcaagtcgc tgatttcaaa aaactgtagt
    10141 atcctctgcg aaacgatccc tgtttgagta ttgaggaggc gagatgtcgc agacagaaaa
    10201 tgcagtgact tcctcatcag gtacaaagcg agcatacaga aaaggtaatc ctctgacgct
    10261 tgcagaacgt cagcaggcat ccctggcaag aaaacgagct acacataaag aactcagagt
    10321 gttcattcct gctgcgttaa aagcgcaatt gcaggtgatg tgtgaagctg agggcgttac
    10381 ccaggctgag atgattgccg aactgataaa gcagaagagt gcttttagct aactaaaatg
    10441 tagcgacatc acattcttgc ctgtttctgg tgtgcgtgat agattgctga tcgtttaagg
    10501 aattttgtgg ctggccacgc cgtaaggtgg cagggaactg gttctgatgt ggatttacag
    10561 gagccagaaa agtgaaaacc ccgataatct tcgacaagtt tggcgactga gaagattacc
    10621 ggggccatct aaaaaccgta tagccaacaa ttcagctatg cggggagtat agttatatgc
    10681 ccggaaaagt tcaagacttc tttctgtgct cactccttct gcgcattgta agtgcaggat
    10741 ggtgtgactg atcttcaaca aacgtattac cgccaggtaa agaacccgaa tccggtgttt
    10801 acaccccgta aaggtgccgg aacgctgaag ttctgcgaaa aactgatgga aaaggcggtg
    10861 ggcttcactt cccgttttga tttcgccatt catgtggcgc atgcccgttc gcgtggtctg
    10921 cgtcgacgca tgccaccggt gctgcgtcga cgggctattg atgcgctcct gcaggggctg
    10981 tgtttccact atgacccgct ggccaaccgc gtccagtgct ccatcaccac gctggccatt
    11041 gagtgcggac tggcgacgga gtctgctgcc ggaaaactct ccatcacccg tgccacccgg
    11101 gccctgacgt tcctgtcaga gctgggactg attacctacc agacggaata tgacccgctt
    11161 atcgggtgct acattccgac cgatatcacg ttcacatctg cactgtttgc tgccctcgat
    11221 gtatcagagg aggcagcggc cgccgcgcgc cgcagccgtg tggtatggga aaacaaacaa
    11281 cgcaaaaagc aggggctgga taccctgggc atggatgaac tgatagcgaa agcctggcgt
    11341 tttgttcgtg agcgttttcg cagttatcag acagagctta agtcccgggg aataaagcgt
    11401 gcccgtgcgc gtcgtgatgc ggacagggaa cgtcaggata ttgtcaccct ggtgaaacgg
    11461 cagctgacgc gcgaaatcgc ggaagggcgc ttcactgcca atcgtgaggc ggtaaaacgc
    11521 gaagttgagc gtcgtgtgaa ggagcgcatg attctgtcac gtaaccgtaa ttacagccgg
    11581 ctggccacag cttccccctg aaagtgacct cctctgaata atccggcccg cgccggaggc
    11641 attcgcacgc ctgaagcccg ccggtgcaca aaaaaacagc gtcgcatgca aaaaacaatc
    11701 tcatcatcca ccttctggag catccgattc cccctgtttt taatacaaaa tacgcctcag
    11761 cgacggggaa ttttgcttat ccacatttaa ctgcaaggga cttccccata aggttacaac
    11821 cgttcatgtc ataaagcgcc agccgccagt cttacagggt gcaatgtatc ttttaaacac
    11881 ctgtttatat ctcctttaaa ctacttaatt acattcattt aaaaagaaaa cctattcact
    11941 gcctgtcctg tggacagaca gatatgcacc tcccaccgca agcggcgggc cccgaccgga
    12001 gccactttag ttacaacaca caaaaacaac ctccagaaaa accccggtcc agcgcagaac
    12061 cgaaaccaca aagcccctcc ctcataactg aaaagcggcc ccgccccggc ccaaagggcc
    12121 ggaacagagt cgcttttaat tatgaatgtt gtaactacat cttcatcgct gtcagtcttc
    12181 tcgctggaag ttctcagtac acgctcgtaa gcggccctca cggcccgcta acgcggagat
    12241 acgccccgac ttcgggtaaa ccctcgtcgg gaccactccg accgcgcaca gaagctctct
    12301 catggctgaa agcgggtatg gtctggcagg gctggggatg ggtaaggtga aatctatcaa
    12361 tcagtaccgg cttacgccgg gcttcggcgg ttttactcct gtatcatatg aaacaacaga
    12421 gtgccgcctt ccatgccgct gatgcggcat atcctggtaa cgatatctga attgttatac
    12481 atgtgtatat acgtggtaat gacaaaaata ggacaagtta aaaatttaca ggcgatgcaa
    12541 tgattcaaac acgtaatcaa tatctgcagt ttatgctggt tatgctggct gcatggggca
    12601 ttagttgggg agccagattt gtcatggagc aggccgttct gctttatgga tcaggaaaaa
    12661 actatttgtt cttcagtcat ggtactgttc tgatgtacct gctgtgtgtt ttcctggtat
    12721 accgccgttg gatagctccg ctaccggtcg ttggtcagct gcgcaacgtt ggcgtaccgt
    12781 ggctggtcgg tgcgatggcc gtggtgtatg tcggtgtatt tctgctcggt aaggcgctgg
    12841 ctctgcctgc tgagccattt atgacgaaac tttttgccga taagtccata cctgacgtga
    12901 tcctgacgtt gctgaccatc tttatccttg ccccgttgaa tgaggaaacg ctgttccggg
    12961 ggattatgct gaacgtcttc cgttcacggt actgctggac gatgtggctg ggggcgctga
    13021 taacgtcgtt gttgttcgtc gccgcgcaca gccagtatca gaacctgctg acactggcag
    13081 aactgttcct ggtggggttg attacatcag tggccaggat cagaagtggt ggcctgctgc
    13141 tgccggtatt gctgcatatg gaagcaacca cgctgggttt actgtttggt tgaaagttat
    13201 atttttatta aacattgtgc gttaaagcct ggtgtgtttt tttagtggat gttatattta
    13261 aatataactt ttatggaggt gaagaatgca taccacccga ctgaagaggg ttggcggctc
    13321 agttatgctg accgtcccac cggcactgct gaatgcgctg tctctgggca cagataatga
    13381 agttggcatg gtcattgata atggccggct gattgttgag ccgtacagac gcccgcaata
    13441 ttcactggct gagctactgg cacagtgtga tccgaatgct gaaatatcag ctgaagaacg
    13501 agaatggctg gatgcaccgg cgactggtca ggaggaaatc tgacatggaa agaggggaaa
    13561 tctggcttgt ctcgcttgat cctaccgcag gtcatgagca gcagggaacg cggccggtgc
    13621 tgattgtcac accggcggcc tttaatcgcg tgacccgcct gcctgttgtt gtgcccgtaa
    13681 ccagcggagg caattttgcc cgcactgccg gctttgcggt gtcgttggat ggtgttggca
    13741 tacgtaccac aggtgttgta cgttgcgatc aaccccggac aattgatatg aaagcacggg
    13801 gcggaaaacg actcgaacgg gttccggaga ctatcatgaa cgaagttctt ggccgcctgt
    13861 ccactattct gacttgaaca tggggtttga ggggcaactg gatgaaaacg tacgatttag
    13921 ggcactaaaa ccgctgttgt cccaccattc tggtgattcc caaacgttat ttggctaaaa
    13981 agtagttttg atgtggttta ttttccgaat tccaagcgca gccctacttt cttgtgcgta
    14041 tttgcgtgtt ttgcgcagtt ttgaagtt
//
LOCUS       DAEBXN010000106        13514 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.106, whole genome shotgun sequence.
ACCESSION   DAEBXN010000106 DAEBXN010000000
VERSION     DAEBXN010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13514
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.106"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            173..886
                     /gene="ydiV"
                     /locus_tag="KJL99_003272"
     CDS             173..886
                     /gene="ydiV"
                     /locus_tag="KJL99_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HBD4437888.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQLLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFVWLNLTPQVATLLLDRDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLEKGFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            950..2386
                     /locus_tag="KJL99_003273"
     CDS             950..2386
                     /locus_tag="KJL99_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HBD4437889.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFVVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLREEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(2390..2581)
                     /gene="hemP"
                     /locus_tag="KJL99_003274"
     CDS             complement(2390..2581)
                     /gene="hemP"
                     /locus_tag="KJL99_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HBD4437890.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(2713..3759)
                     /gene="aroH"
                     /locus_tag="KJL99_003275"
     CDS             complement(2713..3759)
                     /gene="aroH"
                     /locus_tag="KJL99_003275"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HBD4437891.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTEIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLAFAVDTRF"
     gene            complement(3916..4749)
                     /gene="ppsR"
                     /locus_tag="KJL99_003276"
     CDS             complement(3916..4749)
                     /gene="ppsR"
                     /locus_tag="KJL99_003276"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HBD4437892.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            5081..7459
                     /gene="ppsA"
                     /locus_tag="KJL99_003277"
     CDS             5081..7459
                     /gene="ppsA"
                     /locus_tag="KJL99_003277"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HBD4437893.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPSEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(7516..9162)
                     /gene="fadK"
                     /locus_tag="KJL99_003278"
     CDS             complement(7516..9162)
                     /gene="fadK"
                     /locus_tag="KJL99_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HBD4437894.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNIPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWLDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRSIKLLSVY
                     GSTESSPHAVVNLDNSLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEMAAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASDKIQKFLLRKDIIRRLTQDVCEEIE"
     gene            complement(9219..9512)
                     /locus_tag="KJL99_003279"
     CDS             complement(9219..9512)
                     /locus_tag="KJL99_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HBD4437895.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILTENPDINEFRKLMKA
                     CPAGLYKQDDVGNIHFDPAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(9509..10798)
                     /locus_tag="KJL99_003280"
     CDS             complement(9509..10798)
                     /locus_tag="KJL99_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBD4437896.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSANSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(10854..11792)
                     /locus_tag="KJL99_003281"
     CDS             complement(10854..11792)
                     /locus_tag="KJL99_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HBD4437897.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGVKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(11812..12576)
                     /locus_tag="KJL99_003282"
     CDS             complement(11812..12576)
                     /locus_tag="KJL99_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HBD4437898.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            12896..13396
                     /locus_tag="KJL99_003283"
     CDS             12896..13396
                     /locus_tag="KJL99_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBD4437899.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDALAYTLAIL
                     VMLPTY"
     gene            13430..>13514
                     /locus_tag="KJL99_003284"
     CDS             13430..>13514
                     /locus_tag="KJL99_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBD4437900.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQ"
BASE COUNT         3276 a         3360 c         3486 g         3392 t
ORIGIN      
        1 aattccgtaa atggattatt cgtttataat caggataatc taaaaaaagg ccccctgaaa
       61 aagtggttaa catggaacaa atgaaagtta ctccttctgt aattgttatg tcttatggcc
      121 tcatcccttt tagccggata ctgaaaaaca tccttcgaga gggacggtta ccatgaagat
      181 ttttttggag aatctttatc attctgattg ctatttcctg ccgataagag ataaccagca
      241 actccttgtc ggcgttgagt taattaccca tttctccagt gaagatggca cggttcgtat
      301 cccgacgagt cgggttatag cgcaacttac cgaggagcaa cattggcaat tattttcaga
      361 gcagttggaa ttactaaaat catgccaaca cttttttatt caacataaac tctttgtctg
      421 gcttaattta acgccacaag tggctacatt attattagac agggataatt acgcaggcga
      481 actattaaaa tatccgttta ttgagctgtt gattaatgag aattacccgc atctcaatga
      541 aggaaaagat aatcgcgatc ttttgtcctt atcgcaaatg tatccccttg tgttaggcaa
      601 tttgggagca ggtaatagca caatgaaagc ggtttttgat ggtcttttca cccgcgtaat
      661 gctggagaaa ggttttattc agcaacaaat cacgcatcgc tccttcgaac cgtttattcg
      721 tgcgatccag gcgcaaattt ccccttgttg caattgcatc atcgcggggg gcattgatac
      781 gccagaaata ttggcacaga ttaccccctt cgatttccat gctctccagg gatgcctgtg
      841 gcctgccgtt cctataaatc agataacgac gttggttcag cgataaccct cctgtttgct
      901 ggtgtttaag acgagagtaa ccgtctacac tatcaaacag ataggatcta tgaccctgtc
      961 ttttattacc cgctggcgcg atgaattgcc agaaacctat acagcacttt cccctacgcc
     1021 tttaaataat gcccggctga tttggcataa taccgaactc gctaacacgc tgagtattcc
     1081 atcgtcgctg tttaaaaatg ccgcaggtgt ctggggcggc gaaaccctac tgcctggtat
     1141 gtcaccactg gcacaggttt acagtggtca tcagttcgtc gtctgggcgg gccaactggg
     1201 tgatgggcgc ggcattttac ttggcgaaca actgcttgct gatggcacta caatggactg
     1261 gcatctgaaa ggtgctggcc tgacgcctta ttcgcgaatg ggtgatggac gggcggtttt
     1321 acgttcgacg atacgagaaa gtctcgccag tgaggcgatg cattatctgg gcattccgac
     1381 gacccgcgcg ttaagtatcg tcaccagcga ttcgccagtg tatcgggaaa cggtggagcc
     1441 aggcgcgatg ctgatgcgtg tggcaccaag tcatctgcgc tttggtcatt tcgaacattt
     1501 ttactatcgc cgcgagccgg aaaaggttcg tcagttggct gactttgcca ttcgtcatta
     1561 ctggtcacat cttgaagacg atgaggacaa ataccgtctc tggtttaacg atgttgtcgc
     1621 acgtaccgcg tcgttaattg cccaatggca gacggtcggc tttgcacatg gggtgatgaa
     1681 taccgacaac atgtcgctgc tgggactgac gcttgattac gggccgtttg gttttcttga
     1741 tgattacgaa cccggtttta tttgtaatca ctcggatcat caagggcgtt atagctttga
     1801 taatcaacct gctgtcgcgt tgtggaattt acagcgtctg gcgcagacat tgtcaccatt
     1861 tgttgccgta gatgccctga atgaggccct ggacagctat cagcaggttt tgttgacgca
     1921 ttatggacaa cggatgcggc agaaactggg cttcatgacg gagcaaaaag aggataacgc
     1981 gctactgaat gaattattca gtctgatggc gcgagagcgc agcgattata cccgcacatt
     2041 ccgcatgctg agtctgaccg agcagcacag cgcggcgtca ccgctacgtg aggagtttat
     2101 tgatcgtgcg gcatttgatg actggtttgc ccgttatcgg gggcgtttgc aacaagacga
     2161 ggttagcgat agtgagcgtc agcaactgat gcaaagcgtt aacccagctc tggtattgcg
     2221 caactggttg gcgcaacggg cgattgaagc ggcagaaaag ggtgatatga tggaattgca
     2281 ccgcctgcat gaggcgttgc gaaatccttt cagcgacaga gatgatgact atgtcagtcg
     2341 tccacctgac tggggtaaac ggctggaagt cagctgctcg agttaaaggc tacttagtta
     2401 acagcagctt gccagcctga gttttacgga gcagatattc ttgcccgtca tgatcgataa
     2461 tcagtttgcc atccggacct aacagtgtct ggctggtaat tcgccggatg ggctgcgggg
     2521 aagcggtctt gtgattggca tccgtttcag gcgtgagttt tctgctatcc gtataacgca
     2581 taaaaattaa taataattga tataacaatg attcctatta tcaatattat ggcagaccag
     2641 agcaagcagt aatgtgttga gaagacaaca agttgcgcag aaatgcccat cccatcagga
     2701 atgggcacgc attcagaagc gggtatctac cgcaaaggcg agtttttcga ccaggcgttc
     2761 ggtatcttcc cagcccagac acgggtcggt aatggattga ccgtaagtga gcggctgacc
     2821 gccgacgatt ttttgcgttc cttcgcgcag gaaactttcc gccataattc cagcaatctc
     2881 cgtagagcca ttgcggattt gctgacaaat atcctcacaa acttctaact ggcgacggtg
     2941 ctgcttctgg cagttaccgt ggctgaaatc caccaccaga tgttcaggta aatcaaactc
     3001 gtgcagcgta tcgcaggctg cggcgatatc atcggcatga taattcggtt ttttgccgcc
     3061 acgcataata atgtggccat acgggttgcc gctggtctga tagatggtca tctgaccatt
     3121 tttgtctggc gagaggaaca tatggctggc gcgggctgcg cggatagcat ccacggcaat
     3181 ccgcgtgttg ccatcggtac catttttaaa acctaccgga caggagagtg ctgaagccat
     3241 ttcgcggtgg atctgacttt cggtagtacg agcgccaatc gcgccccaac tgattaaatc
     3301 agcaataaac tgaccggtta ccatatcgag gaactcagtc gcggtaggga cacccagctc
     3361 atttacctgt aaaagtaatt tgcgcgccag ctccagaccg tgatttaccc gatagctgcc
     3421 gtttaaatcc ggatcggaga ttagcccttt ccagccgaca acagttcgtg gtttttcaaa
     3481 ataggtgcgc attacgattt ccagccgtga ctggtactgg ttgcgcagcg actgcagacg
     3541 ggtggcgtac tccattgcag cggtgagatc gtggatcgag caggggccaa taatgaccaa
     3601 cagtcgctta tcttcaccat tcagtatttt ttcaattctg cggcgggagt cggtgacatg
     3661 ggtggcgacg ccaggcgtta cgggataccg tagcgcgagt tcggcgggcg ttaccaggct
     3721 ctcaatacgc gcagtacgga gttcgtcagt tctgttcatt acaagtctca gaaatttgtt
     3781 gcttctacgg caggactacc ggaaagtgat gcgcatcacg ataaaccaat cgcagctgat
     3841 ttcaagtgcg aggtgtgtca cacctcgccg ggtggttagc atgataacaa aaaaacaaac
     3901 caatgcacta gttctctagt acattcggcg actaaggccc atgatatcga ggatcttggt
     3961 ggcaatctct tctaccgaat aattggtact gttaatccac gggatctgat ttttacggta
     4021 caaggcttcc acttccgcga cttccatcct gcactgacga agcgaggcat agcgactgtt
     4081 ctcccgacgt tcctcgcgaa tcgccgccag acgttccggg tcgatagtca ggccgaacaa
     4141 tttatgctga agcggtttga gcgacgcggg tagcaccaga ttatccatat cgtcggcaat
     4201 aaaggggtag tttgccgcgc ggataccaaa ttgcattgcc agatacagac tggtgggggt
     4261 tttaccacag cgagaaacac cgagcaggat cacctgagcc tgatccagat tgcgcaacga
     4321 aatgccgtca tcgtgggcga gggtgtaatc aatcgccgca atgcgcgcat catatttatt
     4381 gagattatta gggttaaggc catgggtacg atgagcaatc ggcgttggat ccagtttcat
     4441 ctcttgttgt agcggggcaa ccagcgcctg aacgatatcc tggcaaaagc cttcactttg
     4501 caagatgatg gcgcgaatct ccggcaacac gatggagtag aagaccagcg ggcgcacgcc
     4561 tgtctggtga taaattgcgt caatctgatc cttcactgcc cgtgcacggc tctcattttc
     4621 gacaaacggc agcgtgatgc tgctgatagt gacgggaaat tgtgacatta ctgcgtgtcc
     4681 taatacctcc gcagttattg ccgtaccatc agaaatataa aaaacgtggc gatcaacagc
     4741 attatccatt ttgttcttcc cgtgaagcag acataattct atgaaagcat aaattaaaag
     4801 cgcacagaag cgtaggacgt aatgtctggt ttataaaatg aaccttcatt ttatttttta
     4861 taaaaacagc atttcatttt tatggtttcg tttataccga tggtttatgt ggaaattgtc
     4921 gaatagagca ggtttgcgca acgctgggat cagtcttaaa aagtaaaaaa atatatttgc
     4981 ttgaacgatt caccgttttt ttcatccggt taaatatgca aagataaatg cgcagaaatg
     5041 tgtttctcaa accgttcatt tatcacaaaa ggattgttcg atgtccaaca atggctcgtc
     5101 accgctggtg ctttggtata accaactcgg catgaatgat gtagacaggg ttgggggcaa
     5161 aaatgcctcc ctgggtgaaa tgattactaa tctttccgga atgggtgttt ccgttccgaa
     5221 tggtttcgcc acaaccgccg acgcgtttaa ccagtttctg gaccaaagcg gcgtaaacca
     5281 gcgcatttat gaactgctgg ataaaacgga tattgacgat gttactcagc ttgcgaaagc
     5341 gggcgcgcaa atccgccagt ggattatcga cactcccttc cagcctgagc tggaaaacgc
     5401 catacgcgaa gcctatgcac agctttccgc cgatgacgaa aacgcctctt ttgcggtacg
     5461 ctcctccgcc accgcagaag acatgccgga cgcttccttt gccggtcagc aggaaacctt
     5521 cctcaacgtt cagggttttg acgccgttct cgtggcagtg aaacatgtat ttgcttctct
     5581 gtttaacgat cgcgccatct cttatcgtgt gcaccagggt tacgatcacc gtggcgtggc
     5641 gctctccgcc ggtgttcagc ggatggtgcg ctccgacctc gcatcttctg gcgtgatgtt
     5701 ctccattgat accgaatccg gctttgacca ggtggtgttt atcacttccg catggggcct
     5761 tggggaaatg gtcgtgcagg gtgcggttaa cccggatgag ttttacgtgc ataaaccgac
     5821 actggcggcg aatcgcccgg ctatcgtgcg ccgcaccatg gggtcgaaaa aaatccgcat
     5881 ggtttacgcg ccgacccagg agcacggcaa gcaggttaaa atcgaagacg taccgcagga
     5941 acagcgtgac atcttctcgc tgaccaacga agaagtgcag gaactggcaa aacaggccgt
     6001 acaaattgag aaacactacg gtcgcccaat ggatattgag tgggcgaaag atggccacac
     6061 cggcaaactg ttcattgtgc aggcgcgtcc ggaaaccgtg cgctcacgcg gtcaggtcat
     6121 ggagcgttat acgctgcatt cacagggtaa gattatcgcc gaaggccgtg ctatcggtca
     6181 tcgcatcggc gcgggtccgg tgaaagtcat ccatgacatc agcgaaatga accgcatcga
     6241 acctggcgac gtgctggtta ctgacatgac cgacccggac tgggaaccga tcatgaagaa
     6301 agcatctgcc atcgtcacca accgtggcgg tcgtacctgt cacgcggcga tcatcgctcg
     6361 tgaactgggc attccggcgg tagtgggctg tggagatgca acagaacgga tgaaagacgg
     6421 tgagaacgtc actgtttctt gtgccgaagg tgataccggt tacgtctatg cggagttgct
     6481 ggaatttagc gtgaaaagct ccagcgtaga aacgatgccg gatctgccgt tgaaagtgat
     6541 gatgaacgtc ggtaacccgg accgtgcttt cgacttcgcc tgcctgccga gcgaaggcgt
     6601 gggccttgcg cgtctggaat ttatcatcaa ccgtatgatt ggcgtccacc cacgcgcact
     6661 gcttgagttt gacgatcagg aaccgcagtt gcaaaacgaa atccgcgaga tgatgaaagg
     6721 ttttgattct ccgcgtgaat tttacgttgg tcgtctgact gaagggatcg cgacgctggg
     6781 tgccgcgttt tatccgaagc gcgtcattgt ccgtctctct gattttaaat cgaacgaata
     6841 tgccaacctg gtcggtggtg agcgttacga gccagatgaa gagaacccga tgctcggctt
     6901 ccgtggtgcg ggccgctatg tttccgacag cttccgcgac tgcttcgcac tggagtgtga
     6961 agcagtgaaa cgtgtgcgca acgacatggg gctgaccaac gttgagatca tgatcccgtt
     7021 cgtgcgtacc gtagatcagg cgaaagcggt ggttgaagaa ctggcgcgtc aggggctgaa
     7081 acgtggcgag aacgggctga aaatcatcat gatgtgtgaa atcccgtcca acgccttgct
     7141 ggccgagcag ttcctcgaat atttcgacgg cttctcaatt ggctcaaacg atatgacgca
     7201 gctggcgctc ggtctggacc gtgactccgg cgtggtgtct gaattgttcg atgagcgcaa
     7261 cgatgcggtg aaagcactgc tgtcgatggc tatccgtgcc gcgaagaaac agggcaaata
     7321 tgtcgggatt tgcggtcagg gtccgtccga ccacgaagac tttgccgcat ggttgatgga
     7381 agaggggatc gatagcctgt ctctgaaccc ggacaccgtg gtgcaaacct ggttaagcct
     7441 ggctgaactg aagaaataaa ataaatcccc ggcggcgttc agtcgccggg gttatgtgat
     7501 ccccgaagat gaaacttatt caatctcttc acagacatcc tgcgttaatc gccgtataat
     7561 atcttttctt aacaaaaact tttgtatttt atctgaggca gtgcgcggta gtttttcgat
     7621 taccacgata tgttcaggat atttatattt tgcgacccgt ttacggctaa aaaaagccgc
     7681 catctcttcc agcgataatg aatgatgcgg cgctttcagc acgacataag cgcatgatcg
     7741 ttcacctaaa cgttcatcgg gcattgcaac cacacaggca tcgtgaattt taggatgctg
     7801 caataaaata tcttccactt cacggctgct aatattttcg ccgccgcgga caataatatc
     7861 ttttttgcgt ccggtaattt ttatatagcc agcctcatcc atacggcaga gatcgccgct
     7921 gtaataccag ccttcttcat ccagcgcacg ggcggttaat tcaggttcat caaaataccc
     7981 cataaacaca ttggggccac gcgatgcttc ttcaccttcg caacctggcg gtaaggtctt
     8041 gcgtgcgtca tcgaccactt taatctctac acctgcggca gcgtaaccat cggtatgcat
     8101 aaagcgcgac aaagaattat cgagattcac caccgcatgc ggcgaacttt ctgtggaacc
     8161 ataaacactt aataatttaa tgctgcgctg ctggcattcg cgcgccactt ttttggggat
     8221 tgtggtaccg ccgcaaagaa agaagcgcag cgctgaaagg tctgcgggtt gtttctctag
     8281 taaattcaaa agatcataga caaacggcgt tgcgcctagc atacaggtgc aacgctgctg
     8341 ctcaagcagc gcgagacacg catcaggagt gaaaatatct aacaacacgc tgcgagcgcc
     8401 aattaagaat ggtgccgtta cgccatgcag aaagcccgtt gcgtgaccaa gtggcgcagg
     8461 catcataaag acatccagcc aggtcagatt cagccgcgcg caataagccc gctcactggc
     8521 gagaatattg ttatgcgtta gcatcacgcc ctttggcaga ccctcggttc cggaggtaaa
     8581 cagcaccgca gctaattcat cgccgtgggt cgttatcgcc gtggtcagtg ggatattgtc
     8641 ggcgataatc tgacttaatg agagggaaga ggtggcggga gccagtttgt ccacgccgac
     8701 aatttgttgt agttgtggaa gctgattttg cagcggcagg attaaatcta ccggacgcgt
     8761 ttgtttaaac aacgtcggtg caaagaacat ttttgcctga cacttattga gcacccacac
     8821 cagttctgct tcccgccagg aaggcaacag cggcacggaa accgcaccga ttttcaggca
     8881 ggcaagatag ataacggtaa attcacacca gccaggcagt tgaaatgcga tgcgatcgcc
     8941 tgattcaata cccttcgcta acatccagtt tgccagacag ctcgcggcgt gatcgagcgc
     9001 gctataggtg tacgatgcac catgattatc gaccacggca attttgtctg gcatcgcacg
     9061 agcggtctgc tgccagtaat cggccagtga agcatcgccc catagccctt gctgacgata
     9121 cgccgcacga cgttgttcgt taaacgttaa tgtcactttc attttggact ctcgaaacag
     9181 aacatcaggc ccgagttgcg ggcctgtggg atgcatgatt agccgtagcg aaaatcaata
     9241 ccgaaggtgc ctgcgggata ttgccactgt tcgagaatag tgttaccgca cagcacccga
     9301 caggtgccgc actccagaca accggcggga tcaaaatgaa tgtttcctac gtcatcctgc
     9361 ttataaagtc cggcagggca ggctttcatt aatttacgga attcattgat atcgggattt
     9421 tccgtcagaa tgatatgcgg gtggccctca tcaacatgga atttattgac gcctaatttg
     9481 atgtcgacgt taaccgtagc gttctggctc atagcgcggt tgctccctta atgccatctt
     9541 tcagcaagtt gatcagccca attttcttcg cgtgtcccat gatcattttg cgtaccggct
     9601 ggtttggttt gccgtcaatg gtgaacatct cgttcatgat gtcggctacc attcgtgggt
     9661 attggctaaa caggcgcggg ttttccatca gcgccgggat cttgcgaaaa tgctgcatat
     9721 cacgcatgac gcagctttgt tccagctcac gtttgtattg cgccagactg ttggcggaga
     9781 aatccgcgcg ttctttggcg gcgatcaccg ttgtggcggc agcctgagcc gatgcaatgg
     9841 ctaaatccat gccgcggacc gtaaaaccca aattcaggca gaagcctgcg gcgtcaccaa
     9901 cgatcatcac gccctcgtta accagttgcg gcaccattgc cagaccgcct tccggcacca
     9961 tatgcgcgga atattcaagc agtttgccgc cgctaatcag cgggcgaatg gcggggtgtt
    10021 gtttaaaatc ttccagcatt tgcggcacgc ttttttgcgc atgggcgata tcacccaatc
    10081 cacaaaccag ccccaaggat atggaatcct tgttggtata gagaaatccc ccgcccatca
    10141 ggccgtcaga aggggaaccg gcaaacagcc aggcggcacc ttcctcgccc gtaatattaa
    10201 agcgatcgtt gatctgttct ggtgtgaggc caataacctc tttaacacca acagcgtaat
    10261 gatgcggatc ggaagcggga accattccca gcgagcggcc aagcatcgag ttaacgccat
    10321 cagccagaat caccacattc gcttcgagaa tatcatcccc agcctgcacg ccagtgacct
    10381 tgtttccttc acgaaccagc gcgtcgacgc gaacgcctgg gataaactgc gcgcccgcct
    10441 gctcggcttg ttccatcaac cacgggtcca gacgattacg caatacggta taagatgcgt
    10501 gttgaggaac atctggttgc tcgcggtgaa aatcgagggt aacggcgctt tcttcggtta
    10561 agaaggagat tttctcgcgt gtgaccttgc gttctaccgg cgctgatact gcaaagcctg
    10621 gaatgattgc ttcaagtgtg tgggcataaa gacgcccgcc ggtcatgttt ttacatccgg
    10681 cactgtcgcc gcgttctatc accaggacat ccagcccggc tcgtgccatg accagtgcgg
    10741 caacgctacc agcaacgccc gcaccgacca caatggcatc aaatttgtca tccgacatgc
    10801 gttatgctcc cttcctgctg cgtttacagc gggagatgtg gttgaaaggg gaattagcgg
    10861 ctcaactggc taatcagggc agggacgact ttgtagatat cgcccaccag accgtagtcg
    10921 gcatagttga agattggcgc atttttatct ttattgatgg cgacaatcac ttttacgccg
    10981 ttgccgccaa ccatatgttg gatctgcccg gagatcccca gcgtcaggta gagatcggat
    11041 ttcagcaaca cgccggagac accgatataa cgttcacgct ccatccagtt ctcgccttcg
    11101 gcaattggac gtgaacagcc gacttcagca ttcagcaccg ccgccagttc gtggaccatt
    11161 ttcaggtcat cctgcgccgc cagaccacga ccgacgccaa ccacacgttt tgctttgctc
    11221 aggtccacgc tgcttgcggc tttcgcacgg cgttcgcgac agagaatttc atgacgcgga
    11281 gcaacataag gtaccgtttc tgtcgggcac tgatgagagg tatcactggt gcacggttcc
    11341 tgaacaccgg gtgcaagggt aatgatcgcc agcgggctgt tgatcttttc ctgagcgaac
    11401 gctaacccgc catacatccg gtgttcggcg caaatgtgac catcgacaat atccaccgcc
    11461 gtggcatcgt tcaccagcgc cgcattcagt tgcacactta accgtgctgc cagagcttta
    11521 ccacgtttcg tcgcggccaa cagcaacata gcggggtgtt tatctttcag cagggcagca
    11581 atgctttcgg cgtaattttc agtgcgttgc agcgcgtcgt tttgctcaag aacataaata
    11641 cattttggac cataaggcat aactgcctgc gcctggtcgg tattttgtac aatggcatac
    11701 acctgttggc cccattgctg agcgccgcca aacagttcag cataacgttc aggattatcg
    11761 ctaaagaccc agacgctgtt taattgactc atcgtttctt ctccgtaggc ttcagttcag
    11821 ggctttcttc agatgttccg ccagctcggc aatggcctcc ggcgaatcgt tatcgagaat
    11881 gatgtgctta cgttctgttt gcggcggtac gcgaatgccg accagttcag caagtggcgc
    11941 gctctggctc cagtcaatat cacttgcctg ccactgattt accggttttt tacccgcgcc
    12001 gagaatggct ttcatcgaag gaatgcgtgg cacgttaata tcggaggtga cgcagagcac
    12061 ggctggaaca gagagttcaa taacttcaac atcatcttca agcgtgcgtt caatcaccag
    12121 tgtattgccc tgacgctgaa tagcactcac tgcattaatc accggaagtt gcagaatttc
    12181 tccgaccagc aagccaacct gctgggcata aaggtcgccg gaaccttcac caaagatcag
    12241 taaatcgaag ccgatctttt caattgctgc cgccagcgct tttgcggtat cgagaggcag
    12301 tgcatgttca agttgcgcat cctgcaccaa atacaggctg tgcggcccgc gggatagcac
    12361 gtctttgcgc actttcgagt tctgcaacaa tgagccgcca acggtcagcg cggctatctc
    12421 atcgtcatct gttgcgagct ggcttgcagc ttcaatggca ttgagatcga actggctgat
    12481 tttggcgtcg gcattgtcga aattcagggt gtattctgga gtgacaacaa tgtcctgttc
    12541 ttcaggcacc agcttaaagc aggttattat tttcatgatt tctcctgtga gtcgcatacg
    12601 gaaccatctc cgatggaagg taaaaatggc ctgaaaatag tgtgcgatag cggtgcgggg
    12661 ggggggggcg acggagcaag ctgttttcct tgccaacaat cttgcaaaaa aaatgcgatt
    12721 gctaaaacaa aagtgcaatt tgcggcaata tagttattcc atgaccgtac aatcttgcat
    12781 tcagtgtaaa taatcttgtt tagctctcgc taaacgcggt tgtataaccg caatacacgc
    12841 cgtattttat ccggcatata ttgtttttgc ttgctcatgg aatagggagg ttgtcatgta
    12901 tcaacgctgt tttgataatg ccagtgaaac gctgtttgtc gccggtaaaa cgccacggct
    12961 ttcacgtttt gcatttagcg atgatccaaa atgggagtct ggacatcacg ttcatgacaa
    13021 tgaaaccgag ctgatttacg tcaagaaagg ggttgcaagg tttaccatcg attcttcgtt
    13081 atatgtcgca catgcggatg acattgtggt gatagaacgc ggcaggctgc atgcggtggc
    13141 ctctgacgtt aacgatccgg caacgacgtg tacctgtgcg ctgtacggct ttcagtttca
    13201 gggggctgag gaaaatcagt tactgcaacc gcattcttgt ccggtaattg ccgcggggca
    13261 gggaaaagaa gtcataaaaa ccttatttaa tgagctaagt gtgattttgc cgcaaagtaa
    13321 aaatagccaa acatcttcgt tatgggatgc attagcctat acattagcaa ttctggtaat
    13381 gctgccaact tactgattta gtgtatgatg gtgattttaa ggtgcttgcg tggcttccat
    13441 ttccatcaga tgtccttcct gctccgctac tgaaggcgtg gtgcgtaacg gcaaaagcac
    13501 tgccggacat cagc
//
LOCUS       DAEBXN010000107        13209 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.107, whole genome shotgun sequence.
ACCESSION   DAEBXN010000107 DAEBXN010000000
VERSION     DAEBXN010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13209
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.107"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(16..999)
                     /gene="kdgT"
                     /locus_tag="KJL99_003285"
     CDS             complement(16..999)
                     /gene="kdgT"
                     /locus_tag="KJL99_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HBD4437901.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(1259..1879)
                     /gene="sodA"
                     /locus_tag="KJL99_003286"
     CDS             complement(1259..1879)
                     /gene="sodA"
                     /locus_tag="KJL99_003286"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HBD4437902.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            2164..3198
                     /gene="rhaT"
                     /locus_tag="KJL99_003287"
                     /pseudo
     CDS             2164..3198
                     /gene="rhaT"
                     /locus_tag="KJL99_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(3195..4061)
                     /gene="rhaR"
                     /locus_tag="KJL99_003288"
     CDS             complement(3195..4061)
                     /gene="rhaR"
                     /locus_tag="KJL99_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HBD4437903.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(4117..4953)
                     /gene="rhaS"
                     /locus_tag="KJL99_003289"
     CDS             complement(4117..4953)
                     /gene="rhaS"
                     /locus_tag="KJL99_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HBD4437904.1"
                     /translation="MTVLHSVDFFPSCNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            5241..6710
                     /gene="rhaB"
                     /locus_tag="KJL99_003290"
     CDS             5241..6710
                     /gene="rhaB"
                     /locus_tag="KJL99_003290"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HBD4437905.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMPQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIELMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            6707..7925
                     /gene="rhaA"
                     /locus_tag="KJL99_003291"
                     /pseudo
     CDS             6707..7925
                     /gene="rhaA"
                     /locus_tag="KJL99_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
     gene            8167..8991
                     /gene="rhaD"
                     /locus_tag="KJL99_003292"
                     /pseudo
     CDS             8167..8991
                     /gene="rhaD"
                     /locus_tag="KJL99_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
     gene            9001..9315
                     /gene="rhaM"
                     /locus_tag="KJL99_003293"
     CDS             9001..9315
                     /gene="rhaM"
                     /locus_tag="KJL99_003293"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HBD4437906.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGVHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            9616..10062
                     /locus_tag="KJL99_003294"
     CDS             9616..10062
                     /locus_tag="KJL99_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBD4437907.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQEFIHQLQQGDTDQVLALLNQTLSS"
     gene            10073..11524
                     /locus_tag="KJL99_003295"
     CDS             10073..11524
                     /locus_tag="KJL99_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HBD4437908.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISLALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            11514..12584
                     /locus_tag="KJL99_003296"
     CDS             11514..12584
                     /locus_tag="KJL99_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBD4437909.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGFVAPHALTEKQRQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKNCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RGFLTTLTAEKVNAFSQFRQVD"
     gene            12584..>13209
                     /locus_tag="KJL99_003297"
     CDS             12584..>13209
                     /locus_tag="KJL99_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBD4437910.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPQLKQRYERTCCLASRPGLGHFIDETEEKLVILLANLLRKDPFLIPLA
                     GITRDNLQHLSTACDNQHRWPLIQGDYLSSLILAIYALRNQLTDEWPQ"
BASE COUNT         3146 a         3451 c         3480 g         3132 t
ORIGIN      
        1 attaaggaag caggtttatt tcaccgtacc taaaatttcg attttcgctg ctctggcttt
       61 gactttacgt gaccagatag aggtaagaat tggcaccaga atcgaagtca caatgaccgc
      121 cgtcgctacc agcgaagttg ctgccggagc catcggttta aacgcaggca ccatttctgc
      181 aatcagcaca ggtgtcgcta ccgcggcccc tgcggaactg gaagcggcaa ttccggcagt
      241 gccatcgcca ccgccaatca atttatcggc gataatcaac ggaataccgg tcacgataat
      301 tactgccaca cccaacagga tccccagcaa accagtctgg gcaatcacag tcaaatcaat
      361 ggtattgccc agcgcgaagg caaagaatgg aatcagcgtt tgcaccgctt tgctgaaaaa
      421 ttctcgtaat tcagggtcaa gattccccag ggcaaagccc accaggaacg gtaatactgc
      481 gccgacgaaa acatgcggtt caaacgaggc aatcccggca gtgcccagaa taatcatcgt
      541 catgagcggc ccggactcca acgacatcaa cacaaatgcc ccagcttctt ctttcgtacc
      601 gtactgctgc atgatggaag cgtaaagtcc gccgttggtc atatccatcg ccgccaccag
      661 cgccagcgtt gaaagtccgg caaagaatcc aacttcaaca ccatgttccg gaataatgcg
      721 cgaggcaatt gccgcaacca cccacgcgac ggcaatttta gttaccacca gcgtaccgga
      781 tttacgcagt accgttcccg tcgcgcttaa ttttattgac gcccccatgc aaaaaaacca
      841 caccgccaga atgggcaccg taccggtaat catcccgttg gtgaatgatc caaaatattt
      901 ccccgccccc ggcgagaagg tgtggcacag tgcgccaagg aatagcggga cgagcatcat
      961 cccccccggg attttctcaa tcgagcgttt tatctgcatt tccatcacct gtcggcatat
     1021 ttttaagggc tgatgcgtaa ttcctcaatg gagcatcagt gttgatggaa acgaagatag
     1081 acattcattt aatcaataaa agtgatcaaa atcacatcaa ttgaaacgct gttttatttt
     1141 tataggccca ttgtttttat tttggaattg atcacaaaaa aaccaccgcc gttggcgatg
     1201 gttcattttt taagctgata tgcggcctat acgcctcatt gcagcaggcg gcaaatgatt
     1261 attttttcgc cgcaaaacgt gccgctgctt cgtcccagtt caccacgttc cagaactctt
     1321 tgatgtagtc cggacggcgg ttctggaatt tcaggtagta agcatgttcc cacacatcca
     1381 ggcccagaat cgggaagccg gaagcgccag aaatagcttc acccatcagc ggagaatcct
     1441 ggttagcagt agaaaccacc gccagtttat cgcctttcag caccagccat gcccagccgg
     1501 aaccaaagcg ggaagctgcc gctttttcaa attctgcttt gaagttatca acggagccga
     1561 agtcacgttc gatagccgct ttcaggtcac cctgcagggt ggtgcctttt ttcagacctt
     1621 tccagaacag gctgtggtta gcgtgaccgc cagcgttgtt gcgcagtacg gttttcttgt
     1681 ctgctggcag ctggtccagt ttggtgatca gctcttcaac cggcaggttg gcaaattctg
     1741 gcaggctttc cagcgccgcg ttggcgttgt ttacgtaggt ctgatggtgt ttggtgtggt
     1801 ggatttccat ggtctgctta tcgaagtgcg gttccagggc atcgtaagca tacggcaggg
     1861 atggcagggt atagctcata ttcatctcca gtattgtcgg gcggccgatt gttaatgccg
     1921 cgtaagcagt tggttcatta tagttaatta aatgatattg aaaatgatta tcaatgccgt
     1981 acttttcgta agggtatggt tttgcaggaa aatgcccgag atgtgaagca aatcacccac
     2041 ttaatgccgt gattgccagt aaatcgacaa cggcggcaac aggcgaaagg ttaatcgaca
     2101 gcacgatttt tacactcatc tcgtcggaga tgtgaagcga cgaaaaatga tgaggataag
     2161 aagatgagta acgcgattac gatggggata ttttggcatt tgatcggcgc ggccagtgca
     2221 gcctgttttt acgctccgtt caaaaaagta aaaaaatggt catgggaaac catgtggtca
     2281 gtcggtggga ttgtttcgtg gattattctg ccgtgggcca tcagcgccct gttactaccg
     2341 gatttctggg cgtattacag ctcgtttagt ctctctacgc tgctgcctgt ttttctgttc
     2401 ggcgctatgt gggggatcgg taatatcaac tatggcctga ccatgcgtta tctcggcatg
     2461 tcgatgggaa ttggcatcgc cattggcatt acgttgattg tcggtacgct gatgacgcca
     2521 attatcaacg gcaatttcga tgtgctgatt aacaccgaag gcggacgcat gacgttactc
     2581 ggcgttctgg tggcgctgat tggcgtaggg attgtaactc gagccgggca gttgaaagag
     2641 cgcaagatgg gcattaaagc cgaagagttc aatctgaaaa aagggctggt gctggcggtg
     2701 atgtgcggca ttttctctgc cgggatgtcc tttgcgatga acgccgcaaa accgatgcat
     2761 gaagccgctg ccgcacttgg cgtcgatcca ctgtatgttg ctctgccaag ctatgttgtc
     2821 atcatgggcg gcggcgcgat cattaacctc ggtttctgtt ttattcgtct ggcaaaagtg
     2881 aaggatttgt cgctaaaagc cgacttctcg ctggcaaaac cgctgatcat tcacaatgtg
     2941 ttactctcgg cactgggcgg gttgatgtgg tatctgcaat tctttttcta tgcctagggc
     3001 cacgcccgca ttccggcgca gtatgactac atcagttgga tgctgcatat gagtttctat
     3061 gtattgtgcg gcggtatcgt cgggctggtg ctgaaagagt ggaacaatac aggacgccgt
     3121 ccggtaacgg tgttgagcct cggttgtgtg gtgattattg tcgccgctaa catcgtcggc
     3181 atcggcatgg cgaattaatc tttctgcgaa ttgagatgac gccactggct gggcgtcatc
     3241 ccggtttccc gggtaaacac caccgaaaaa tagttactat cttcaaagcc acattcggtc
     3301 gaaatatcac tgattaacag gcggctatgc tggagaagat attgcgcatg acacactctg
     3361 acctgccgca gatattgatt gatggtcatt ccagtctgct ggcgaaattg ctgacgcaaa
     3421 acgcgctcac tgcacgatgc ctcatcacaa aatttatcca gcgcaaaggg acttttcagg
     3481 ctagccgcca gccgggtaat cagcttatcc agcaacgttt cgctggatgt tggcggcaac
     3541 gaatcactgg tgtaacgatg gcgattcagc aacatcacca actgcccgaa cagcaactca
     3601 gccatttcgt tagcaaacgg cacatgctga ctactttcat gctcaagctg accgataacc
     3661 tgccgcgcct gcgccatccc cacgctacct aagcgccagt gtggttgccc tgcgctggcg
     3721 ctaaatcccg gaatcgctcc ctgccagtca agattcagct tcagccgctc cgggcaataa
     3781 ataatattct gcaaaaccag atcgttaacg gaagcgtagg agtgtttatc gtcagcatga
     3841 atataaaaga gatcgccacg ggtaatgcga taaggacgat cgttgagtac atgcagacca
     3901 ttaccgcgcc agacaatcac cagctcacaa aaatcatgtg tatgttcggc aaagacatct
     3961 tgcggataac ggtcagccac agcgactgcc tgctggtcgc tggcaaaaaa atcatctttg
     4021 agaagtttta actgatgcgc caccgtggct acctcggcca gagaacgaag ttgattattc
     4081 gcaatatggc gtacaaatac gttgagaaga ttcgcgttat tgcagaaagc catcccgtcc
     4141 ctggcgaata tcacgcggtg accagttaaa ctctcggcga aaaagcgtcg aaaagtggtt
     4201 actgtcgctg aatccacagc gataggcgat gtcagtgacg ctggcctcgc tgtggcgtag
     4261 tagatgtcgg gctttcatca gtcgcaggcg gttcaggtat cgctgaggcg tcagtcccgt
     4321 ttgctgctta agctgccgat gtagcgtacg cagtgaaaga gaaaattgat ccgccacggc
     4381 atcccaattc acctcatcgg caaaatggtc ctccagccag gccagaagca agttgagacg
     4441 tgatgcgctg ttttccaggt tctcctgcaa actgctttta cgcagcaaga gcagtaattg
     4501 cataaacaag atctcgcgac tggcggtcga gggtaaatca ttttcccctt cctgctgttc
     4561 catctgtgca accagctgtc gcacctgctg caatacgctg tggttaacgc gccagtgaga
     4621 cggatactgc ccatccagct cttgtggcag caactgattc agcccggcga gaaactgaaa
     4681 tcgatccggc gagcgataca gcacattggt cagacacaga ttatcggtat gttcatacag
     4741 atgccgatca tgatcgcgta cgaaacagac cgtgccaccg gtgatggtat agggctgccc
     4801 attaaacaca tgaatacccg tgccatgttc gacaatcaca atttcatgaa aatcatgatg
     4861 atgttcagga aaatccgcct gcgggagccg gggttctatc gccacggacg cgttacaaga
     4921 cggaaaaaaa tccacactat gtaatacggt catactggcc tcctgatgtc gtcaacacgg
     4981 cgaaatagta atcacgaggt caggttctta ccttaaattt tcgacggaaa accacgtaaa
     5041 aaacgtcgat ttttcaagat acagcgtgaa ttttcaggaa atgcggtgag catcacatca
     5101 ccacaattca gcaaattgtg aacatcatca cgttcatctt tccctggttg ccaatggccc
     5161 attttcctgt cagtaacgag aaggtcgcga attcaggcgc tttttagact ggtcgtaatg
     5221 aaattcagca ggatcacatt atgacctttc gcaattgtgt cgccgtcgat ctcggcgcat
     5281 ccagtgggcg cgtgatgctg gcgcgttacg agcgtgaatg ccgcagcctg acgctgcgcg
     5341 aaatccatcg ttttaacaat gggctgcata gccaaaacgg ttatgtcacc tgggatgtgg
     5401 atagcctgga aagtgccatt cgccttggat taaacaaggt gtgcgaggaa gggattcgta
     5461 tcgatagcat tgggattgat acctggggcg tggactttgt gctgctcgac caacagggtc
     5521 agcgtgtggg cctgcccgtt gcttatcgcg atagccgcac caatggcctg atgccgcagg
     5581 cacaacagca actcggcaaa cgcgatattt atcagcgtag cggcatccag tttctaccct
     5641 tcaatacgct ttatcagttg cgtgcgctga cggagcaaca acctgaactt attccacaca
     5701 ttgctcacgc tctgctgatg ccggattact tcagttatcg cctgaccggc aagatgaact
     5761 gggaatatac caatgccacc accacacaac tggtcaatat caatagcgac gactgggacg
     5821 agtcgctact ggcgtggagc ggggccaaca aagcctggtt tggtcgcccg acgcatccgg
     5881 gtaatgtcat tgggcactgg atttgcccgc agggtaatga gattccggtg gtcgccgttg
     5941 ccagccatga taccgccagc gcggttatcg cctcgccgtt aaacggttca cgcgccgctt
     6001 atctctcttc tggcacctgg tcattgatgg gcttcgaaag ccagacgcca tttaccaatg
     6061 acacggcgct ggcagccaac atcaccaatg aaggcggggc ggaaggtcgc tatcgggtgc
     6121 tgaaaaatat tatgggctta tggctgcttc agcgagtgct tcaggaacgg caaatcaacg
     6181 atctcccggc gcttatcgcc gcgacacagg cacttccggc ctgccgcttc atcatcaatc
     6241 ccaatgacga tcgctttatt aatcctgacg agatgtgcag cgaaattcag gctgcgtgtc
     6301 gggaaacggc gcaaccgatc ccgggaagtg atgctgaact ggcgcgctgt attttcgaca
     6361 gtctggcgct gctgtatgcc gatgtgttgc atgagctggc gcagctgcgc ggtgaagatt
     6421 tctcgcaact gcatattgtc ggcggcggct gccagaacac gctgctcaac cagctatgcg
     6481 ccgatgcctg cggtattcgg gtgatcgccg ggcctgttga agcctcaacg ctcggcaata
     6541 tcggcatcga gttaatgacg ctggatgaac tcaacaatgt ggatgatttc cgtcaggtcg
     6601 tcagcaccac cgcgaatctg accaccttta cccctaatcc tgacagtgaa attgcccact
     6661 atgtggcgca gattcactct acacgacaga caaaggagct ttgcgcatga ccactcaact
     6721 ggaacaggcc tgggagctgg cgaaacagcg tttcgcggcg gtggggattg atgtcgagga
     6781 ggcgctgcgc caacttgatc gtttacccgt ttcaatgcac tgctggcagg gcgatgatgt
     6841 ttccggtttt gaaaacccgg aaggttcgct gaccgggggg attcaggcca caggcaatta
     6901 tccgggcaaa gcgcgtaatg ccagtgagct acgtgccgat ctggaacagg ctatgcggct
     6961 gattccgggg ccgaaacggc ttaatttaca tgccatctat ctggaatcag atacgccagt
     7021 ctcgcgcgac cagatcaaac cagagcactt caaaaactgg gttgaatggg cgaaagccaa
     7081 tcagctcggt ctggatttta acccctcctg cttttcgcat ccgctaagcg ccgatggctt
     7141 tacgctctcg catcccgacg acagcattcg ccagttctgg attgatcact gcaaagccag
     7201 ccgtcgcgtt tcggcctatt ttggcgagca actcggcaca ccatcggtga tgaacatctg
     7261 gatcccggat ggtatgaaag atatcaccgt tgaccgtctc gccccgcgtc agcgtctgct
     7321 ggcagcactg gatgaggtga tcagcgagaa gctaaaccct gcgcaccata tcgacgccgt
     7381 tgagagcaaa ttgtttggca ttggcgcaga gagctacacg gttggctcca atgagtttta
     7441 catggggtat gccaccagcc gccagactgc gctgtgcctg gacgccgggc acttccaccc
     7501 gactgaagtg atttccgaca agatttccgc cgccatgctg tatgtaccgc agttgctgct
     7561 gcacgtcagc cgtccggttc gctgggacag cgatcacgta gtgctgctgg atgatgaaac
     7621 ccaggcaatt gccagtgaga ttgtgcgtca cgatctgttt gaccgggtgc atatcggcct
     7681 tgacttcttc gatgcctcta tcaaccgcat tgccgcgtgc gttgctggaa cctaccgctg
     7741 agctgcgcaa gctggaagcg gcgggcgatt acactgcgcg tctggcactg ctggaagagc
     7801 agaaatcgtt gccgcggcag gcggtctggg aaatgtattg ccaacatcac gatacgccag
     7861 caggtagcga atggctggag agcgtgcggg cttatgagaa agcgattttg agccagcgtg
     7921 ggtaaacact gccggatgcg gcacgagcgc cttatccggc ctacgggtcg aatgtccatg
     7981 cgggctgtca ccctcacccc ggccctctcc ctgaaagggc gagggagaaa aacatagcct
     8041 cgtgcggcaa cggagctgaa caccgctccg gacagtcccc tcgcccctac ggggagaggg
     8101 ttagggtgag gggaacaagc cgcaccatgt ctaaacaagg caacctaatt tacaggaaca
     8161 cagaacatgc aaaacattac ccagtcctgg tttgtccagg gaatgatcaa agccaccacc
     8221 gatgcctagc tgaaaggctg ggacgagcgc aacggcggca acctgacgct gcgcctggat
     8281 gacgccgata tcgcgcttta tcacgacaac ttccacccac aaccgcgcta tattccgctc
     8341 agccaaccca tgcctttact ggcaaatacg ccgtttattg tcaccggctc cggcaaattc
     8401 ttccgtaacg tccagctcga tcctgcagct aacttaggcg tcgtaaaagt cgacagcgat
     8461 ggcgcgggct accatattct ctgggggtta accaacgaag ccgttccaac ttctgaactt
     8521 ccggctcact ttctctccca ctgcgagcgc attaaagcca ccaacggtaa agatcgggtg
     8581 atcatgcact gccacgccac caacctgatc gccctcacct atgtactgga aaacgacacc
     8641 gcggtcttta ctcgccaact gtgggaaggc agcaccgaat gtctggtagt attcccggat
     8701 ggcgttggca ttctgccgtg gatggtgccc ggcacggacg aaatcggcca ggcgaccgca
     8761 caagagatgc aaaaacattc gctggtgttg tggcccttcc acggtgtgtt cggttccgga
     8821 ccgacgctgg atgaagcctt tggtttaatc gacaccgcag aaaaatcagc acaagtatta
     8881 gtgaaggttt attcgatggg cggcatgaaa cagaccatca gccgtgaaga gttgatagcg
     8941 ctcggcaagc gcttcggcgt tacaccacta gccagtgcgc tggcgctgta aggagcaaac
     9001 atgatccgca aagcctttgt catgcaggta aaccccgacg cccacgaaga gtatcagcgt
     9061 cggcataatc ccatctggcc ggaactggaa gcagtgttga aatctcacgg tgtgcataac
     9121 tacgccattt acctcgacaa agcgcgtaat ctgctgtttg ccatggtaga gattgaatct
     9181 gaagaacggt ggaatgcggt agccagtact gatgtttgcc aacgttggtg gaaatatatg
     9241 accgatgtta tgcccgctaa cccagataac agcccggtga gtagcgagct gcaagaagtg
     9301 ttttacctgc cgtaattgcc caatgtgccg gagatgcctc cggcacaata ttttaaattt
     9361 cgatttgaaa aattaaacat attacatctc ccggtaagtt atatttccct gatacattgt
     9421 gagtaaatca caaaaataat gaataaccca ttaatgattc atgtggttta tttaaataac
     9481 cattatgtgc attactccgc aaatctgacc tttcactctg tctcaaattg tcattgttta
     9541 ccgacatatc gcctctcatc acttaatcgc tccaccatta caattgatta aaatatattt
     9601 tatggagtcc gattaatggc agctcttact gcaagctgta ttgacctgaa tattcagggc
     9661 aatggcgctt attccgttct gaagcagttg gcgacaatag cgttacaaaa cggttttatc
     9721 accgactcac accagttcct gcaaaccctg ctgctgcgcg aaaaaatgca ctctactgga
     9781 tttggttccg gtgtcgcagt gccgcacggt aaaagcgcct gcgttaaaca accgttcgta
     9841 ttattcgccc gcaaagcgca ggctattgac tggaaagcca gcgatggcga agacgtcaat
     9901 tgctggatct gcctcggcgt gccgcaaagc ggcgaagagg atcaggtcaa aatcatcggc
     9961 acactgtgtc gcaaaattat tcaccaggaa tttattcatc aactgcaaca gggcgatacc
    10021 gaccaggtgc tggccttgtt aaatcaaacc ctcagctcat aaggaagtgg cgatggagtc
    10081 atccttacgt attgtcgcga tcaccaactg ccccgccggg atcgctcaca cctacatggt
    10141 ggcggaagcc ctggaacaga aagcccgttc tctcggccat accataaaag tggaaactca
    10201 agggtccagt ggcgttgaaa accgcttatc cagcgaagag attgccgctg ccgattacgt
    10261 cattcttgct accgggcgtg gcctgagcgg tgatgatcgc gcgcggtttg ccgggaagaa
    10321 agtttatgag attgccatct ccctggcgtt gaaaaatatc gaccagattt tcagcgaatt
    10381 accgacaaac tcgcagcttt ttgccgcaga tagcggcgtg aagcttggca aacaggaagt
    10441 gcaaagcggc agcgtgatga gccacctgat ggcgggcgtc tctgcggcgc tgccgtttgt
    10501 catcggcggt ggtatcctgg tggcgcttgc caacatgctg gtgcagttcg gtttgccgta
    10561 taccgatatg tcgaaaggcg cgccgtcgtt tacctgggta gttgaatcca tcggttacct
    10621 tggcttcacc tttatgatcc ccatcatggg cgcttatatt gcctcgtcga ttgccgataa
    10681 accggctttc gctccggcgt ttctggtttg ctatctggcg aacgacaaag cgctgctcgg
    10741 cacccagtcg ggtgcgggat tcctcggcgc ggtggtgctg gggctggcga ttggctattt
    10801 cgtcttctgg ttccgcaaag tgcgccttgg caaagcatta caaccgctgc tcggttcaat
    10861 gctgatcccg ttcgttactc tgctggtttt cggcgtgctc acttactacg ttatcgggcc
    10921 ggtgatgtcc gacctcatgg gcgggctgct ccacttcctg aataccattc ctccatcaat
    10981 gaagtttgcg gcagcgtttc tggtcggtgc gatgctggcg ttcgatatgg gcggccccat
    11041 caataaaacc gcctggttct tctgcttctc actgctggaa aaacacattt atgactggta
    11101 cgccatcgtc ggcgttgtcg cgttgatgcc gcccgttgcg gcgggtcttg cgacgttcat
    11161 tgcgcccaaa ctgtttaccc gacaggaaaa agaggctgcc agtagtgcca ttgtggtcgg
    11221 tgccacagtc gcaaccgaac cggctatacc ttacgctctg gcagctccgt tgccgatgat
    11281 taccgccaat acgctggcgg gcggcattac cggcgtgctg gtgatcgctt tcggaatcaa
    11341 acgtctggca cctggtttgg gtatctttga cccgctgatc ggcctgatgt cgccggtagg
    11401 ttcgttttat ctggtgctgg cgattggcct cgcgctgaac atttcattca ttatcgtcct
    11461 gaaaggattg tggttacgcc gcaaagcgaa agccgcgcaa caggagcttg tccatgaaca
    11521 ttgagttact gcaacagttg tgcgaagcca gcgccgttag cggcgatgaa caggaagttc
    11581 gcgacatcct gataaacacg ctggaacctt gcgttaatga aatcaccttt gatggtctgg
    11641 gcagctttgt tgcccgtaag ggaaataaag gtccaaaagt tgccgttgtc gggcatatgg
    11701 atgaagtcgg ctttatggtc acccacatcg acgagagcgg ttttctgcgt ttcaccacca
    11761 ttggtggctg gtggaatcag tcgatgctca accatcgggt aaccatacgc acacacaagg
    11821 gagtgaaaat ccctggtgtg attggtttcg tcgcgcctca tgcgttaacg gaaaagcaaa
    11881 ggcaacagcc gctgtcattt gatgagatgt tcattgatat tggcgcaaac agtcgcgatg
    11941 aggtggaaaa acgcggtgtg gcgatgggcg attttattag cccggaagcc aattttgcct
    12001 gctggggcga agataaagtg gtcggcaagg cgctggataa ccgcatcggc tgcgcaatga
    12061 tggctgaact attgcagacg gtgaataatc ccgaaattac gctgtatggc gtcggcagcg
    12121 tggaagaaga agttgggcta cgcggggcac aaacctcggc ggaacacatt aaaccggatg
    12181 tagtgatcgt gctggatacc gctgtcgcag gcgatgttcc gggcattgat aacattaaat
    12241 acccgctgaa actgggccag gggccggggc tgatgctgtt tgacaagcgc tacttcccca
    12301 accagaaact ggtagcagcg ttaaaaaact gtgccgcaca taacgattta ccgctgcaat
    12361 tttccaccat gaaaaccgga gcgacggatg gcgggcgcta caacgtgatg ggcggcgggc
    12421 gtccggttgt tgcgctgtgc ctgccaaccc gctatctgca cgctaacagc ggcatgattt
    12481 caaaagccga ttacgatgct ctgctcacgt tgatacgggg ttttctgacg accttaaccg
    12541 cggagaaagt caacgcgttt agccagttcc gtcaggtgga ttaatgctaa acgaacgcca
    12601 gttaaagatt gtcgatctgc tggagcaaca gccgcgcacg cctggtgagc tggcgcaaca
    12661 gactggcgtt tcaggcagga ccatcctgcg tgatattgac tatctcaact ttacccttaa
    12721 cggcaaagcc cgcattttcg ccagtggcag tgcgggctat cagctggaaa tcttcgagcg
    12781 ccgcagcttt tttcagttgc tgcaaaagca cgataacgac gatcggctgc tggcgctgtt
    12841 attactgaat actttcactc cccgtgcgca actcgcctcg gcgcttaatt tgccagaaac
    12901 gtgggtagca gagcgtctgc cccagttaaa acagcgttat gaacgcactt gttgcctggc
    12961 cagccgccct ggtttgggcc atttcattga tgagacagaa gagaaactcg ttatcttgct
    13021 ggcgaacttg ctgcgcaaag atccgttttt aattccgctg gcgggcataa cacgagacaa
    13081 ccttcagcat ttatccacgg cctgcgacaa ccaacaccgc tggccgctca tacagggtga
    13141 ttatctctcc agcctgattc tggcgattta cgccctgcgt aatcaactga ccgatgagtg
    13201 gccgcaata
//
LOCUS       DAEBXN010000108        13104 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.108, whole genome shotgun sequence.
ACCESSION   DAEBXN010000108 DAEBXN010000000
VERSION     DAEBXN010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13104
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.108"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(<1..896)
                     /locus_tag="KJL99_003298"
     CDS             complement(<1..896)
                     /locus_tag="KJL99_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBD4437911.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGG"
     gene            1137..1937
                     /locus_tag="KJL99_003299"
     CDS             1137..1937
                     /locus_tag="KJL99_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBD4437912.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            2015..2785
                     /locus_tag="KJL99_003300"
     CDS             2015..2785
                     /locus_tag="KJL99_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBD4437913.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASALDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFCSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2833..4179)
                     /gene="mltD"
                     /locus_tag="KJL99_003301"
     CDS             complement(2833..4179)
                     /gene="mltD"
                     /locus_tag="KJL99_003301"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HBD4437914.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDDDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAN
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAQRLANN
                     SDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMPDS"
     gene            complement(4251..5006)
                     /gene="gloB"
                     /locus_tag="KJL99_003302"
     CDS             complement(4251..5006)
                     /gene="gloB"
                     /locus_tag="KJL99_003302"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HBD4437915.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVITTPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTALQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSIFPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINGINEETLLQQPEERFAWLRSKKDRF"
     gene            5040..5762
                     /locus_tag="KJL99_003303"
     CDS             5040..5762
                     /locus_tag="KJL99_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBD4437916.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDICLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(5759..6226)
                     /gene="rnhA"
                     /locus_tag="KJL99_003304"
     CDS             complement(5759..6226)
                     /gene="rnhA"
                     /locus_tag="KJL99_003304"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HBD4437917.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            6291..7022
                     /gene="dnaQ"
                     /locus_tag="KJL99_003305"
     CDS             6291..7022
                     /gene="dnaQ"
                     /locus_tag="KJL99_003305"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HBD4437918.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            7155..7231
                     /locus_tag="KJL99_003306"
     tRNA            7155..7231
                     /locus_tag="KJL99_003306"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7189..7191,aa:Asp,seq:gtc)
     gene            complement(7362..8408)
                     /locus_tag="KJL99_003307"
     CDS             complement(7362..8408)
                     /locus_tag="KJL99_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImpA family type VI secretion system protein"
                     /protein_id="HBD4437919.1"
                     /translation="MEIFLSDPISAECPCGPDLEYDPEYLLLFTRAAPREEAQYGDFV
                     STPENINWAELERDAHRLLMRSKDIRILVVLLRCRIQQAGARGLSEALTLLETLCSTY
                     PDAIHPQLLATEDITAEDAAVARSNALAALLDHEGVMADIRGITLSNNAAMRLQVRDV
                     ERSLSALRPADALAPESVRQQLADLEARGTLPLDAFRQAAETTERLQRHARETLNDQA
                     PDFSRLTQLLALLPGAVQSTTPEILPQPQAEQPENATIVHTEQMQAEQIALPVHIAEE
                     ISPMTGAEPRQIRDRNDALERLRVIRRWFEHSEPSSPTIPLLRQAERLVGKRVSEVIN
                     EIPVELLEKWDALE"
     gene            complement(8422..8981)
                     /gene="tssG"
                     /locus_tag="KJL99_003308"
                     /pseudo
     CDS             complement(8422..8981)
                     /gene="tssG"
                     /locus_tag="KJL99_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601590.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
     gene            complement(8978..10861)
                     /gene="tssF"
                     /locus_tag="KJL99_003309"
     CDS             complement(8978..10861)
                     /gene="tssF"
                     /locus_tag="KJL99_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HBD4437920.1"
                     /translation="MDPRLLEYYNRELSYLRETGAEFAARHPKVAARLGMQGTDIADP
                     YVERMVEAFSFLTARTQLKIDAEFPRFTQRLLEVVSPNYVTPTPSMSVAQLHPDTEEG
                     DLAKGFTVPRDTAFFSAIPEGESTACQFRSSQDVTLWPLAIEEARLTAAPPDMPALHR
                     YLPANIHVAGALRITLRTFGELTFSQLAGLDRLPFYLCGEERTASHLLELLHTSAIAP
                     LAGIPGHFDGALDVNLQQPVMYEGLEPDQGLLPLAWNVFHGHNLLHEYFACPERFYFF
                     TPTGLSAGLQKIDGGVAEIVILLNRLPPDWLIHQTNAAQFSLFCTPVINLFPRVTARI
                     DVTHSTTEQHLVVDRTHPLDYEVFSVQEVEGLETDTTRKMAFRPLYHTRNNDEGNHGR
                     YFSLRREPRRLSENARRYGTRTPYTGSEVFLSLVDQYEAPYPENLRHITITAMVTNRD
                     LPCLIARNGRDDLTVDAAIPVAGVGLIRPPRSPQPPMAEREMAWRLIRQLSFNYLPLA
                     DLDHRTGGQALRDLLNLFIPAHDSPQSRQVRSLIGCKTTPVTRRLPGSGLLVYGRGVS
                     CELTVDEEGFSGISPYLFGLVLEHYIARHVSINTFSQMTLHSMQRGKIMTWPVRAGQR
                     GSV"
     gene            complement(10877..11371)
                     /gene="tssE"
                     /locus_tag="KJL99_003310"
     CDS             complement(10877..11371)
                     /gene="tssE"
                     /locus_tag="KJL99_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HBD4437921.1"
                     /translation="MSASRQRPGNHLLPTLFDRLCDDAPNQKRDHGISVSPVQIKEII
                     RRDLSFLLNTVSHEDDIDAARYPYAAASVLNYGLPPLAGSFLYEHKWDDIRRAILRTI
                     TRFEPRLKASTLQIIPLQDERRQSGHNTLQFEIRGEILTQPYPTAFRVRSALDMEQSR
                     ITFF"
     gene            complement(11368..12198)
                     /locus_tag="KJL99_003311"
                     /pseudo
     CDS             complement(11368..12198)
                     /locus_tag="KJL99_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233830.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="impE family protein"
     gene            complement(12185..13099)
                     /gene="tagK"
                     /locus_tag="KJL99_003312"
                     /pseudo
     CDS             complement(12185..13099)
                     /gene="tagK"
                     /locus_tag="KJL99_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804008.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagK"
BASE COUNT         3007 a         3397 c         3485 g         3215 t
ORIGIN      
        1 cctccgggag ctgtttttac atgctcgcta aggaaatcga taaaagcccg gatgcgcgta
       61 cttaccgcac ggtcgctgta atagacggca ctgaatggca tttccactgg caacacttta
      121 tctgccatta actccaccaa ttctccgcga gcgatttctt tgtcgatcat gtagtcggac
      181 aaacacgcaa tcccgttgcc actcaggcaa agctgtttca gtgtttcccc actattggat
      241 gacaaaccgt acttcacctc atgtaattgt ccatcactac aggctatcgg ccaggtattg
      301 agggaagcgg gttcagtgaa tcccaggcaa acatgttgct ttaaatcgtc gatcgtttct
      361 ggcttcccgt agcgggaaat ataatcgggg gaggcgataa tttttcgata actgttaaat
      421 aacggcctgg cacgtaagct ggagtccgtt aacgtaccag cgcgtatcgc gacatccact
      481 tttctttcga tcaaattaat aatcgtttcg gaggagacta gcgataaagt gacttccgga
      541 tagcgttcac ggaaaggctt aattaacggc atcagaaagt gcagcaccac tggagttgcg
      601 gcatcgatcc gtaacagtcc acgcggtgta ttacgcgtct ccataatttc tgattctgcc
      661 gctgccatct cctgcaaaat tgactgtacg cgacgaaaat aacgctcgcc ttcttccgtc
      721 aggctaagtt gccgcgtggt ccgattaagc aggctaacgc caagtttcat ttccagcttt
      781 ttcaccgccc ggcttaccgc tgagtttgct tgccctaatt gttccgctgc ccggctaaag
      841 ctgccgcttt cgacgaccga aacaaaaatg gcgagttctt ccgacgtggc tttcattttt
      901 gctcctgttg caaaatagat gagatatttt gaatttattt gtcattaaac catcaggatg
      961 cgtgatatgt catccgattt aatgttctca ataatgagca aatttctgac aggtgtaagc
     1021 acttgcttac ataacaatat acaattgctc gttgaaagag tgagctaaaa tccctataac
     1081 agtagaaccc tcccgagtgc ggaagggttg acgtaataga ggtttcaaag tcaaaagtgc
     1141 gaaaaaacac ctatgccatg cgctatgttg ccggacaacc tgcggaaagg atcttaccgc
     1201 cggggtcttt tgcgagcatc ggccaggcat taccacctgg agaaccgtta agtaccgaag
     1261 agcgtattcg gatcctggtg tggaacatat acaaacagca acgcgctgaa tggttgtcgg
     1321 tattaaagaa ctacggcaaa gatgcacatc tggtgttatt gcaggaagcg cagacaacgc
     1381 cagagttagt acagtttgcg accgctaact atcttgccgc cgatcaggtg cccgctttcg
     1441 tgctgccaca acatccttcc ggtgtaatga ccctttcggc ggcgcatcca gtgtattgct
     1501 gcccgttacg tgaacgagaa cccattttgc gtctggcgaa gtcggcactg gtgacggtct
     1561 atccattacc tgacacccgc ctgttgatgg tggttaatat acacgccgtc aacttcagtc
     1621 tgggcgtgga tgtctatagt aagcagttac ttcctattgg tgatcagata gctcaccaca
     1681 gcggcccggt cattatggcg ggagatttca atgcctggag ccgtagaagg atgaacgcgt
     1741 tatatcgctt tgcgcgggaa atgtcgctgc gccaggtgcg ttttaccgat gatcagcgcc
     1801 gtcgggcgtt tggccgcccg ctcgattttg ttttctaccg tggtctgaac gtcagcgaag
     1861 cttctgtatt ggttacgcgc gcttccgatc acaatccgct actcgttgaa ttcagtcccg
     1921 gcaagcctga taaataaggt atgtcaggtc tgccacaggg cagaccaacg tttggcgctg
     1981 tccttttata ctcaaacaac aaaaggacag cgcaatgaca acacaatccc accatgacca
     2041 cgtagaaaag cagtttagct ctcaagcctg cgaatatctg accagtaccg tacatgcatc
     2101 cggacgagat ttacagcgcc tggcggtgcg tctggctgat tatcctggtg caagtgcact
     2161 tgatatgggc tgcggagcag ggcatgccag ctttgttgct gcgcaaaacg tgagcgcggt
     2221 ggtggcgtat gacttatctg cccaaatgct ggatgtcgtg gcacaagctg ccgaagcccg
     2281 gcaactgaaa aatatcacca cccgccaggg atatgccgaa agtctgccat ttgccgataa
     2341 cgcatttgat attgttatca gccgttattc tgcccatcac tggcatgatg ttggtgcagc
     2401 actgcgtgaa gtgaaccgga tattgaaacc tggcggtagg atgattgtga tggacgtaat
     2461 gtctccgggt catccagtgc gcgacatctg gttacagacg gtagaagcat tacgcgatac
     2521 ctctcacgta cgaaactacg ccagcggtga gtggttgagg ttaatcaatg aagccaatct
     2581 gatagttgat aatttaatta cagataagtt accgctggaa ttttgttcat gggtcgcgag
     2641 aatgcgtacg ccagaagcgt tagtagacgc tattcgcatt taccaacaga gcgcatcgac
     2701 agaggtgaga acgtattttg ccttgcagaa tgacggctct ttcaccagtg atatcatcat
     2761 ggtagaagca cataaagcgg cataaataaa aaaggcaccg ggggaatcgg taccttttta
     2821 ttatctggtt tgtcaggaat ctggcatgtt gttgtttttc acaaacaacg tcagcttgtc
     2881 gcctggttgc agattcgcag tgtcgctgtt ccagcgcatc acatctttga tgttcacgcc
     2941 gtggcgttta gcaatgcttg aaagcgaatc gcctttgcgc acacgatacg taatgctatc
     3001 gctgttgttt gccagtcgct gtgcaccaat cgtcaaactt tggcctggct tcagcttaga
     3061 tccgcgcagt ttgttccact gctgcaaatc tttggtgctt acgccgagac gtgaagcgat
     3121 gcttgaaagc gtgtcgccag agcgtacggt gtaaacacgg ctgttaagcg gcgtattgtt
     3181 ggcaaccagc gtcgactgta cagcagcaat ttcgcctgaa gcgagagatt cacgcagttg
     3241 atctgcatgc ttctttggca ccatcacgta ctgcggccca cttgcgccca gcgtggagcc
     3301 tttcacgcca gcgttgaatg tcttcagctt gctgacggaa atccccgcca tatctgcaac
     3361 cttcgccatt tcaaccgggc tactcaggtg cacacgcgcc agagcacggc tttcatcggt
     3421 cgttggcaga cgtacgccat aacgcttgct gtttttgaga atatcactca atgccagcat
     3481 tttaggcacg tactgcttcg tttcctgcgg caacggtaac gaccagaagt ccgtggattt
     3541 cccacgcgct ttgttcgttt taattgcctt catgacccga ccttcgccgc tgttataagc
     3601 cgctacggtc agaagccagt cgtcgtcaaa cattttgttc agacgctgca tcatattcag
     3661 cgcggcagtt gttgaagcaa caacatcgcg acgcgcgtca taattgcggg tctgtttcaa
     3721 accataattg cgccccgtgc tcggaatgat ctgccagatg cctgcggcat tggcgccaga
     3781 cgttgcgtga ggatcaaaag cgctctccac tatgggtagt agtaccagtt ccataggcat
     3841 gttacgtttt ttaacttgcc ctgctatcca gtacatatac ggctctgccc gtaaagttac
     3901 atcgtggaga tagctcttat tgcgtaaata tttctgtttc tgttcgcgaa tccggtcatt
     3961 ttccggaatt cccatcttta gctcgtcgcc aatgaaagcc cacaagtcac catctggcgc
     4021 gatagacgtc ccatcgtcca tccatcgtgc ctgacttgta aactttgctg cttccccttg
     4081 accagctgca gaaaggctct gtgcgtgctg ttgaacgttg ccggtactct ggcaacccac
     4141 gagcaggaca gaggcgagta atatcgcttt tgccttcatg tgtgtgtcaa tagttgctta
     4201 aaagacgacc gatcataacg gcgaacggag ccgatgacaa gaaagtttta tcagaaccta
     4261 tctttctttg accttaacca tgcaaaacgc tcttcaggtt gttgcaataa tgtttcttca
     4321 ttaattccat taattaaatc aatatcttcc gttcttaaaa aaacattaat ttgccgctca
     4381 tttttcagaa ttacgggtag tgttatttga ttttttgccc gtaactcctt aactttacga
     4441 taataatcat ttatggacaa atcgtgcgga aaaatactca aagcaaactt catatttgat
     4501 aacgtatatt catgagcaca acataccaat gtatcgtcag gtaacgcact tagcttttta
     4561 agtgattgat acatttgtaa agctgtccct tcaaacaacc gaccacaccc accagaaaac
     4621 agcgtgtcgc cgcaaaatag ataaggttta ctgaagtaac agatatgtcc taaagtgtga
     4681 cccggtgtgg taattacact aaattcatgc cctaaaacga aggcagtttc gccatctttg
     4741 actacctgtg ttgttccctt atcttgtgtc tcttgtggac catacactac aatttgcgga
     4801 aacttttcca ccagttcttt tacgccgcca acgtgatcgt ggtgatggtg ggttagaaat
     4861 atggcctccg gttgccagtt attggcggta atggcgttta atactggctc tgcgtctccg
     4921 ggatcgacaa tcaggcagcg acctgcctca tcattcaaaa cccagatgta attgtcatca
     4981 aaggcgggaa tactgttaag attcatagat tacctctcag tgtaaaacgg aaggttgtga
     5041 tgaaaccggc aagagtccct caaactgtcg tggctcctga ttgctggggc gatttgccct
     5101 ggggagagct ttatcgcaag gcgctggagc gccagctcaa cccgtggttc actaaaatgt
     5161 atggttttca tctgcttaag attggcaatt taagcgcaga aatcaattgc gaagcgtgcg
     5221 cggtttctca tcaagtgaat gtttctgcgc aaggaatgcc cgtccaggtg caggcggatc
     5281 cacttcatct tccttttgcc gataaatccg ttgatatttg tctactggca cataccttgc
     5341 cgtggtgcac cgatccgcat cgtttattac gtgaagccga tcgggtactg attgatgatg
     5401 gctggctggt gattagtggc ttcaacccca tcagtttaat gggattacgc aaacttgtgc
     5461 cggtattgcg caaaacctcg ccctataata gccggatgtt tactctgatg cggcaactgg
     5521 actggctctc tttgttgaat tttgaagtgc tgcacgccag ccgtttccac gttctcccgt
     5581 ggaacaaaca cggaggaaaa ctattgaatg cgcatattcc tgcgcttggt tgcttacaac
     5641 ttattgttgc ccggaaacgg actattcctt taacgctaaa tccgatgaaa cagagtaaaa
     5701 acaagccacg aattcgccag gcggttggag ccacccggca atgtcgtaaa ccacaggctt
     5761 aaacttcgac ttggtagccc gtatcttcca gtgtgggatt catcgccgcg gcacgagcca
     5821 gttcatcaca gcgttcgttt tccgggtgtc cggcatggcc tttaacccat tcccatttga
     5881 tttgatgctg ccccaatgca gcatcaagac gttgccagag atcgacattt tttactggtt
     5941 ttttgtctgc tgttttccag ccacgttttt tccagttatg gatccactgg gtgataccct
     6001 ggcggacata ctggctgtcg gtactcaaaa tgacttcgca atgttctttt aacgcctcca
     6061 gcgcgacaat agcggccatc aactccatac ggttgttggt ggtgcgggtg tagcctgcgc
     6121 taaaggtttt ctcgcgtccg cgatagcgta aaatagcgcc gtaaccccca ggtccaggat
     6181 tacccagaca cgaaccatcg gtgaaaattt ctacctgttt aagcatctct ggtagacttc
     6241 ctgtaattga atcgaactgt aaaacgacaa gtctgacata aatgaccgct atgagcactg
     6301 caattacacg ccagatcgtt ctcgataccg aaaccaccgg tatgaaccag attggtgcac
     6361 actatgaagg ccacaagatc attgagattg gtgctgttga agtagtgaac cgtcgcctga
     6421 cgggcaataa cttccatgtt tatctcaaac ccgatcggct ggtggatccg gaagcctttg
     6481 gcgtacatgg tattgctgat gaatttttgc tcgataagcc cacgtttgcc gaagtagccg
     6541 atgagtttat ggactatatc cgcggcgcgg agttggtgat ccataacgca gcgttcgata
     6601 taggctttat ggactacgag ttttcgttgc ttaagcgcga tattccgaag accaatacct
     6661 tctgtaaggt caccgatagc cttgcggtgg cgaggaaaat gtttcccggt aaacgcaaca
     6721 gcctcgatgc gctatgtgcc cgctacgaaa tagataacag taaacgaacg ctgcacgggg
     6781 cattactcga tgcccagatc cttgcggaag tttatctggc gatgaccggt ggtcaaacgt
     6841 cgatggcttt tgcgatggaa ggagagacac agcagcaaca aggtgaagca acaattcagc
     6901 gcattgtacg tcaggcaagt aagttacgcg ttgtttttgc gacagatgaa gagctggcag
     6961 cacatgaagc ccgtctcgat ctggtgcaga aaaaaggcgg aagctgcctc tggcgggcat
     7021 aaatacatgt aaaaggcgct aaaaatagcg acttgggcga tttttgcagc aaacgattca
     7081 aaagatgaga aaaatcgttg acgaaggtcg aggcaatccg taatattcgc ctcgttccca
     7141 acggaacaca acgcggagcg gtagttcagt cggttagaat acctgcctgt cacgcagggg
     7201 gtcgcgggtt cgagtcccgt ccgttccgcc aatattcaga aagcccgaat cagtaatgat
     7261 tcgggctttc gtcgttttgg tgaagtgaga atttttcact ccgaagtaaa acggatatga
     7321 tttaaaaaat ggccagacat tataaattct ggccacttct gttactccag cgcatcccat
     7381 ttctccagca gttctactgg aatttcattt attacctccg agacgcgttt tccgaccagg
     7441 cgttctgcct ggcgcagcaa aggaatggtc gggctgctcg gttcgctgtg ctcaaaccag
     7501 cgacggatga cacgcaggcg ttccagcgcg tcgtttcggt cgcggatctg ccggggttct
     7561 gcgccggtca tcggtgatat ttcctccgcg atatgcaccg gtagcgcaat ctgctctgcc
     7621 tgcatctgtt cagtatgaac gatggtggcg ttttccggct gctcagcctg tggttgtggc
     7681 aggatctccg gcgtggtgct ctgcactgct cccggtaaca gggccagcag ttgcgtcaga
     7741 cgggagaagt ccggtgcctg gtcattcagc gtttcacgcg catggcgttg cagccgctca
     7801 gtggtctctg ctgcctggcg aaaggcatcc agcggcagtg tgccgcgcgc ttccaggtct
     7861 gccagttgct ggcgtaccga ttctggtgcc agcgcatccg ccgggcgtaa tgcagatagc
     7921 gaacgctcca catcccgcac ctgaaggcgc atggcagcat tatttgacag tgtaatgcca
     7981 cgaatatccg ccattacacc ttcgtggtcc agcaacgccg ccagcgcgtt actgcgtgcc
     8041 accgcagcat cttcggcggt gatatcttca gtcgcgagca gttgaggatg gatggcgtcc
     8101 gggtacgtgg agcacaacgt ttccagcagc gtgagcgcct ccgacagtcc cctggcaccg
     8161 gcctgctgga tacggcagcg cagcaggaca accagaatgc ggatatcttt gctgcgcatc
     8221 agcagacgat gggcgtcccg ctcaagctcc gcccagttaa tgttttccgg cgtactgaca
     8281 aaatcgccat attgcgcttc ttcacgcggt gcggcgcggg tgaacagcag caggtattcc
     8341 ggatcgtact ccagatccgg gccgcagggg cattccgccg aaattgggtc agacaggaat
     8401 atttccattt aattatcctc agtgcgccag cgtggtggcc agaccgtccg gattgctgga
     8461 ctcacgcacc agatgggccg atgccgccag acgagcgtga tctggcaacg ggctgtcagc
     8521 gctctcccgc aaatcctgcc cgctgaggct ggcgacataa aaggcaaagc ggtcatgttg
     8581 cggtttatcc agaccgcccc cagcggactg ggcgctgcgc catgcctggt aaaactgcgt
     8641 cagccagcgg tgatgaaaaa gatcagaaaa acgggtcagc gtcggctttc agtccggtta
     8701 cgcgccagtt cggtgtagtg cagcggcagt ggaccgttcg gcccccacag cacgaggctg
     8761 tacagactga tgtgcaggcg cccttcctgc cagcccacac tggcgatttc tcgtggcgca
     8821 aaggtcagcg cgggcgtttg tccgaggcga aatttttcca tccgtggttg ccaggtgttg
     8881 cccgccggaa cggtacacaa ctgcacatcc acgcggcgca tcaggttcag aaaaccgtaa
     8941 cgccaggggg gggcctgcgc ctgagtgaaa tcgttgctca tacgcttccc cgctgtcccg
     9001 ccctgacagg ccaggtcata attttgccgc gctgcatgct gtgcagcgtc atctgcgaga
     9061 aggtattaat ggaaacatgg cgggcgatat agtgttccag caccaggccg aacagatacg
     9121 gactgatgcc ggaaaaacct tcttcatcca ccgtcagttc acaactgacg ccacgcccat
     9181 agaccagtag tccggaaccg ggcagacggc gggtgaccgg ggtcgtttta caaccaatca
     9241 ggctgcgcac ctggcgcgat tgcggactgt catgagcagg aataaacagg ttcagcagat
     9301 ctcgcaacgc ctggccgccg gtacggtgat ccagatcggc cagcggcaga taattaaaag
     9361 ataactggcg gatcaaccgc catgccatct cacgttcagc catcgggggc tgcggcgagc
     9421 gtggcgggcg gataagccct acgcccgcca ccgggatggc tgcatccacg gttagatcgt
     9481 cccggccatt gcgggcaata agacagggca gatcgcggtt agtgaccatg gccgtgatgg
     9541 tgatgtggcg cagattttcc gggtacggcg cttcatactg gtcaaccagc gacaggaaca
     9601 cttccgagcc cgtatagggg gtacgggtgc cgtaacggcg ggcgttttct gataagcggc
     9661 ggggctcccg gcgcagggaa aaataacgtc cgtgattgcc ttcatcatta ttgcgggtgt
     9721 ggtagagcgg gcgaaaggcc atcttccgtg tggtgtcggt ttccagccct tccacttcct
     9781 ggaccgagaa cacctcataa tccagcggat gggttctgtc caccaccaga tgctgttccg
     9841 tcgtgctgtg cgtgacatcg atccgggcgg tgacgcgggg aaacaggttg ataaccgggg
     9901 tacagaacag gctgaactgt gccgcatttg tctggtggat cagccagtcc ggcggcaggc
     9961 ggttcagcag gataacaatc tccgccacgc caccgtcaat tttctgtaat ccggcagaca
    10021 ggcctgtcgg ggtgaaaaaa tagaagcgtt ccggacaggc aaaatattca tgcagcaggt
    10081 tatgaccgtg aaacacgttc cacgccagcg gcagcagccc ctgatccggc tccaggcctt
    10141 cgtacataac gggctgttgc agattcacat ccagcgcgcc gtcaaaatgc cccggaatgc
    10201 cagccagggg cgcgatggcg ctggtatgta gaagttctaa caggtgtgag gccgtacgct
    10261 cctcgccgca gagataaaac ggcagcctgt ccagtccggc aagctgactg aacgttagtt
    10321 caccaaaggt gcgcagggta atacgcagcg ccccggcgac gtgaatgttg gccggcaggt
    10381 agcggtgcag ggcaggcata tccggtggcg cggcggtcag tcgcgcctct tcgatggcga
    10441 gcggccacag cgttacatcc tggctgctgc gaaactggca ggcagtgctt tcgccttccg
    10501 gaatggcgga gaaaaaggct gtgtcgcgcg ggacggtaaa tcctttcgcc agatcgcctt
    10561 cttccgtatc gggatgcaac tgcgccacgc tcattgaggg cgtgggtgtg acgtaattgg
    10621 ggctgaccac ttccagcagg cgctgggtga aacgggggaa ttcagcgtca atttttagct
    10681 gcgtgcgggc ggtcaggaag ctgaaggcct cgaccattcg ctctacatac ggatcggcaa
    10741 tatccgttcc ctgcatgccc agccgggcgg cgactttggg atgacgggcg gcaaattcgg
    10801 ctcccgtttc ccgcagatag ctcagttcgc ggttgtagta ttccagtagc cgtggatcca
    10861 tcggtctctc tctgaattaa aaaaacgtaa tgcgactctg ctccatatcc agcgcgctac
    10921 gtacccggaa tgcagtcgga tacggctgcg tgaggatctc gccgcgaatt tcaaactgta
    10981 atgtgttatg gccactctgg cggcgctcat cctgaagtgg gatgatttgc agagtggacg
    11041 ctttcagccg gggttcaaac cgggtgatgg ttcgcaaaat ggccctgcgg atatcgtccc
    11101 acttgtgctc atacagaaag ctgcccgcca gtggcggcag gccgtagtta agtacggacg
    11161 ctgccgcgta cggataacgt gcagcatcaa tatcatcttc atggctgacg gtgttgagca
    11221 gaaatgacaa atcccggcgg atgatctcct taatctgaac cggactgacg ctgataccgt
    11281 ggtcgcgttt ctggtttggg gcgtcgtcac acaggcggtc aaacagcgtg ggcagcaggt
    11341 gattgcctgg acgctggcgg gaagcgctca cgcgtcgctt ccttcagcgg tgttaaaggt
    11401 acaggtggac aaatccagca ggctgatatc gccgtgaccg gtgagccaca ccctctgacc
    11461 cagcgcctgt accagtgtct ctcccggacc gtcctgccag accgtttccc ggcacagacg
    11521 cagggagtcg gcagcctttt cagagccgct gtagcgggga aacagctagg cactgtgtgt
    11581 atcgccattg ttcagggtga cgttgacggg tttccagatc aaatcgatca gatgcgcagg
    11641 ccgcggcgat ttcagcgaac agatttgtga aaacggcagc cagatatatg ctccgcccgc
    11701 gatcagttcg agtaccggac cggtacggga gtcgctgtcg ctaatccagt caaatgcgcc
    11761 gccattccac tgcccggaag tgtctgttat ggcctcaagc gcgcggctgc ggtgagcatc
    11821 agcttcctgc gcttcaccac gggcgttaca ggcgagggcg gtcagcagat cctccatcca
    11881 ggcgggcggc gggagaataa caccgggccg ctgttcgccc tgaaagcagg cgtggcggta
    11941 tatctcacag cggatcagct cgccaaatac ctgcgcttcg cgagtataat ttgcgtccat
    12001 ccgggcgcat agctggatct gttgcagtgc ccgcgaccag tcgcctgcca cgcacaataa
    12061 ctgaaacagg ctgtggcggc gggaggcctg cgctgggtgc atcctgacct gttgctctgc
    12121 catctgaatg ccctccacca cggaataatc ccgcagcagc gccgacaagg taatcggtag
    12181 ctcgttattg tttgtcatga tatatctctc catgttgtgc cgggtttatc cggtaatggc
    12241 tgtcaatgcc aatgatgcga tgctctttcc gtgtcagatc tggcagtacg gtgttatgcg
    12301 ctgtttccgg ggcgtattcc ggggaaagta aatgcaggat atcaggggga gtcttttcga
    12361 caggccagat agtttcatct tcgccagtgg catccagtac attgagaata gcgtcaatgc
    12421 ttaacccacc agtgaccata tcctgcaatg tctcggcttc ggtgaccagg tcacgcaatg
    12481 aatccagatc ctgcgaggag ggctcttcgt taaaaggaaa aggatcagca tggcgattaa
    12541 gatcttgttc gggagataac aacgcctcgc ggtattcctg ataaagtcgg gtcagggcat
    12601 ttcctttttc cgcaccttct ccggtggagg aggctccatg aacaggaatg atatcgaatg
    12661 gattttgtga tgtcagatgc tgctgcctga accaatccag atccagatat tcggttattg
    12721 cttccgacgt gatggcagtc tgcgatatcg tttttactgt gctcaggttc ggagatgatg
    12781 gattgccatg tacaaggcac tatgaagata acccccattc tatcaggtcg ccttcattca
    12841 gacgcatacg gtgatgaggt tccacaatct ggtcgtttac cgtacagcaa tattcggtag
    12901 actgattgat aatccaccat gcttcttcgt ggcgggtcag agtgagggca atctttacat
    12961 catcattcac cttaaaaaca ggaatccagg gagaggtaac ggtgaagata atttgtgttc
    13021 cttcagtcgc gtaaattgtg ttatcggcga tcccgtgcgc ctgtattttt tggagtcccc
    13081 acgtaatatt tggtttcatt gtgt
//
LOCUS       DAEBXN010000109        13015 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.109, whole genome shotgun sequence.
ACCESSION   DAEBXN010000109 DAEBXN010000000
VERSION     DAEBXN010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13015
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.109"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(<1..319)
                     /locus_tag="KJL99_003313"
     CDS             complement(<1..319)
                     /locus_tag="KJL99_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HBD4437922.1"
                     /translation="MAISDTKLRTIYGKPYSGPQELADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFD"
     gene            complement(525..1244)
                     /locus_tag="KJL99_003314"
                     /pseudo
     CDS             complement(525..1244)
                     /locus_tag="KJL99_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
     gene            complement(1260..1592)
                     /gene="cusF"
                     /locus_tag="KJL99_003315"
     CDS             complement(1260..1592)
                     /gene="cusF"
                     /locus_tag="KJL99_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HBD4437923.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(1616..2998)
                     /gene="cusC"
                     /locus_tag="KJL99_003316"
     CDS             complement(1616..2998)
                     /gene="cusC"
                     /locus_tag="KJL99_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HBD4437924.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPVMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLDDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEIYL
                     YTALGGGWQQ"
     gene            3155..3838
                     /gene="cusR"
                     /locus_tag="KJL99_003317"
     CDS             3155..3838
                     /gene="cusR"
                     /locus_tag="KJL99_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HBD4437925.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKLLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            3828..5270
                     /gene="cusS"
                     /locus_tag="KJL99_003318"
     CDS             3828..5270
                     /gene="cusS"
                     /locus_tag="KJL99_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HBD4437926.1"
                     /translation="MVSKPFQRPFSLATRLTFFISQATIAAFFAFAWIIIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKRQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVITLPA"
     gene            5420..7657
                     /gene="nrfB"
                     /locus_tag="KJL99_003319"
     CDS             5420..7657
                     /gene="nrfB"
                     /locus_tag="KJL99_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
                     /protein_id="HBD4437927.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGNGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRVLKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            7644..10612
                     /gene="nfrA"
                     /locus_tag="KJL99_003320"
                     /pseudo
     CDS             7644..10612
                     /gene="nfrA"
                     /locus_tag="KJL99_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
     gene            10613..11503
                     /locus_tag="KJL99_003321"
     CDS             10613..11503
                     /locus_tag="KJL99_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HBD4437928.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRFQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQTGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDMLYFSLRYLPVAHGILEY"
     gene            11686..12447
                     /gene="envY"
                     /locus_tag="KJL99_003322"
     CDS             11686..12447
                     /gene="envY"
                     /locus_tag="KJL99_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HBD4437929.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKKLTCVKPWSRLATPVIACHSRTPEVFRLAT
                     NHSKQQSSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYSLTPLNYLAKQRQKVMW"
     gene            complement(12493..12569)
                     /locus_tag="KJL99_003323"
     tRNA            complement(12493..12569)
                     /locus_tag="KJL99_003323"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12533..12535),aa:Arg,seq:tct)
     gene            12812..>13015
                     /locus_tag="KJL99_003324"
     CDS             12812..>13015
                     /locus_tag="KJL99_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBD4437930.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFL"
BASE COUNT         3185 a         3375 c         3305 g         3150 t
ORIGIN      
        1 tgtcgaacca gtagtcgaga cactgggcga ccgtcatgga gttctccacc ttctcttcaa
       61 aataagtgcg tggatccgtt cctgagaaat agagctttcg caagtcagca gtgatctgtc
      121 tggcatcctt caaagacagg gatgggtatc gaccaagccc aagtcgatta ggcttgccat
      181 gccagcgata gcggtactgg aactggatga cccccttcgg tgaaattcgt acgctgaggc
      241 catcagcatc agccaattct tgtgggccag aatatggttt accataaata gtacgcagtt
      301 ttgtgtcgct tatagccata aataatattt ggtacgctca gaaataatgg tttggtacac
      361 atctggtaca caatatcaca tggacaacag cgcacagtaa acaactatat ttgacgttcg
      421 tagacatact caggtgaaaa aaaggttgtt tttcgagtta taacagacag ttaattaact
      481 acagtggaca atgctagaca atgacagaca cagataagca acctaccttc ctctttcgcg
      541 accacgttat ctttggcgat attcattccc gcgcgcagat caaacgcggt gatcacgcca
      601 tcaatgggcg ctttgagcgt aaagcgagtc tggatttttt gcgtggcgat caggtggcga
      661 atatccgcct ccggcattcc cgccagtcgc agccgctcaa gaatgccttc agtctgggtc
      721 gccgtaccgc cggtttcgcg caacagtaaa tactcactct gcgcttccac ccagtcggga
      781 atggtcaggt cgagaagcgg tgtgcccttt tgtactttat cgcccacggt aagcggatac
      841 accttgtcga taaacccggc agcgcgagcc tgcacaatgg catactgata ctcgttgtaa
      901 ctgacattcg tcgggaaact ctgggcaaaa gtcagcggcc cgcgcgtgac ggtagccgtt
      961 ttcaccccca gattctgcgt ctgagtcggg tcaatgcgca caccagacgc agaactctct
     1021 tcatcggcat atttcggcac cagatccata tccataaacg gcgatttacc tggtttatcg
     1081 aaccgcgtat tgggatacat cgggtcgtac cagaataaga ttttacgttc tgcggtcggc
     1141 attttttctg cgtgcggttc cgcctggtca aaccaggtaa aacctgccgc tgaaataata
     1201 ccgcccgcga tcatgctgcc gataataagc gcgatttttt tcatctcatt aaacctgggt
     1261 tactggctga ctttaatatc ctgtaataaa gaaaggttgc cctgctggac aaaattaaac
     1321 gccactttgt cgccggtttt aatttcactc attttcgtct gcggggtgat ggtaaagcgc
     1381 atggtcatct ccggccagtt gacggcagca atcggatcgt gatggatggt gatttttttg
     1441 ctttccagat caacaccttt taccacaccg gtggcactaa tgacctgtgg ttgtgcttcg
     1501 ctcatggttt catgatgatg ttcgttagcc tgggcattaa agccaataac ggtaaacaga
     1561 ctgaacattg cgacttgcag tgcttttttc atttattctc tcctggagtt aaaaattatt
     1621 gctgccaacc gccccctaag gcggtataca aataaatttc gttaacctga cgggcataat
     1681 tcaggtcaag taaagtttgt cgggttgcaa ataaagaacg ctcggcatcc agcacttcca
     1741 gataacttac cgcgccgtgc tgatataatg cccgcgcccg ttgcagggta atttgcagcg
     1801 acgccagata acgctgctgg gcgctgattt gatcgtccag gctttgacgt aatgccagcg
     1861 catctgccac ctctttaaag gcgttctgga ttttctgttc ataattcacc accgactgct
     1921 gctggcgaat ttcggcgata tccagattgg cctggttgcg tccggcatta aaaatgggga
     1981 tctcaatttt aggaataaaa ttccacatcc cgctgctagc gttaaataat gacgacagat
     2041 cgctgctggc ggttgatatt ccgctggtca ggctgataga cgggaaaaat gccgcacgcg
     2101 cagcaccaat attagcatta gccgccatta acgcgtgttc agcttccata atatccgggc
     2161 gctgcaataa gatttgcgac gacaagcccg ccggtaattt aacgctttgc aggctgccgc
     2221 tgtttactgt ctgcgcttgc ggtagcttgc cgtagcttcc caataacagt tgcaatgcat
     2281 tattcgcctg cgccagttcc ccctgacgtt tagcgatgtc actgcgggta ctttctatca
     2341 ccccgcgagc ctgttccagc gccagaacat tgctgctacc ggtcaacagt tgtttttcga
     2401 caaacgcata tgactgctga taattacgca gcgtttcttc ggctatttgc aattgcgcat
     2461 acgccagctg ctgattgaaa tagctttgcg cgacattaga aaccagcaaa atatgcaccg
     2521 cgcgctgggc ttcctcagtg gctaaataat tttggcgctc ggcttcgctc atgttcttta
     2581 agcgaccgaa aaaatcgaga tcaaagctgg cgttaaggcc agtcgagaac tcccgcgtcg
     2641 tggctgaatc gcctttaaga ttgccgctcc agctgccgct gccctcgcca ttgagctgtg
     2701 ggtagcggtc ggcatcggtc agacgatatt gcgcccgtgc ctcctgcact ttcagcgtcg
     2761 ccatgcgcaa atcccggtta ttcaccagcg cctcgctaat cagcgtcttc acctgattat
     2821 caacaaaaaa ggtgcgccag cccacgttct ggtagttatc cgctgcgtta accaggccgt
     2881 tctggctgag tgagaactgc tgcggtacgg gcattaccgg acgctgataa tccggtgcca
     2941 gtgaacaacc agttagcgca agggccacac aaaatggcag aagtttacaa ggagacatag
     3001 gctcataatt tctggtgatt ttatgtcgcc aactttactc gccaggctct gattttccgg
     3061 tgacaggaaa atgacaaaat tgtcattttg ccaataagcg attgccatct gatcccgcta
     3121 ctctagaatt gcccgggcaa catgcggagg aaatatgaaa ctgttgattg tcgaagatga
     3181 aaagaaaacc ggagaatact tgaccaaagg gttaaccgaa gccggttttg tggtcgattt
     3241 ggccgacaac gggctgaatg gctaccatct ggcgatgacc ggtgattatg acctgataat
     3301 cctcgatatt atgctgccag acgtgaacgg ctgggatatc gtgcgcatgt tgcgctccgc
     3361 caataaaggg atgccgattc tgttgcttac cgcgcttggc accattgaac atcgcgtcaa
     3421 ggggctggag ttgggggcag atgactacct ggtgaaaccg ttcgcttttg ctgaactgct
     3481 ggcgcgggtg cgcactctcc tgcggcgcgg ggcggcggtg attatcgaaa gtcagtttca
     3541 ggttgccgat ctgatggtcg atctcgtcag ccgcaaagtc acccgcagcg gcacgcgcat
     3601 cactctgacc agtaaagaat ttactctgct ggagtttttt ctccgccatc agggcgaagt
     3661 gctgccccgc tcgcttatcg cctcgcaggt atgggacatg aatttcgaca gcgacactaa
     3721 cgccattgat gtggcggtga agctgctacg cggcaaaatc gacaacgact ttgagccgaa
     3781 gctgattcaa accgtgcgcg gcgtgggtta catgcttgag gtgccggatg gtcagtaagc
     3841 catttcagcg cccgttttcg ctggcaaccc gcctgacctt ttttatcagc caggccacca
     3901 tcgcggcgtt tttcgccttt gcatggatca taatccactc agtaaaagtg cattttgccg
     3961 agcaggacat taatgattta aaagagatta gcgccaccct tgaacgggta ctaaatcacc
     4021 ctgacgaaac gcaagcccga cgcttaatga cgctggaaga tatcgtcagt ggttattcca
     4081 acgtgttgat ttccctggca gatagccacg gcaaaacggt gtatcactcc cccggtgcgc
     4141 ctgatatccg cgagtttgca cgtgatgcca tacccgataa agacgcgcgg ggtggcgagg
     4201 tgtatctcct ttccggcccg acgattatga tgccaggcca cggtcacggg catatggaac
     4261 acagcaactg gcggatgatt aacttgccgg ttggcccgtt ggtggacggc aaaccgattt
     4321 atacgctcta catcgcgctg tcgatcgatt ttcatcttca ttacataaat gatttgatga
     4381 ataaacttat tatgaccgca tcggtaatca gcatcctgat cgtctttatc gtgctgttgg
     4441 cggtacataa aggtcacgcg ccgatccgca gcgtcagccg tcaaatccag aatatcacct
     4501 cgaaagatct cgacgttcgc cttgacccgc agaccgtgcc gattgagctg gaacagctgg
     4561 tactgtcgtt caaccatatg atcgagcgta ttgaggatgt ctttactcgc cagtccaatt
     4621 tctcagcgga tatcgcccac gaaattcgca caccaattac taatctcatt acgcaaacgg
     4681 aaatcgccct cagccagtcg cgcagccaga aggagctgga agatgtgctc tactctaatc
     4741 tcgaagagct gacgcgaatg gcgaaaatgg tcagcgatat gctgtttctc gctcaggccg
     4801 ataacaacca gctaatcccc gaaaagaaaa tgctcaacct ggcggatgaa gtcggcaaag
     4861 tgttcgattt tttcgaggcg ctagcggaag atcgcggcgt ggagttgcgg tttgttggcg
     4921 acaaacgtca ggttgcgggc gatccgttga tgctgcgtcg ggcgctaagc aacctgcttt
     4981 ctaatgccct tcgttatacg ccaaccagag agacaattgt agtacgctgt cagacggtcg
     5041 atcaccaggt gcaagtttcc gtcgaaaacc ccggtacgcc cattgcgccc gagcacttac
     5101 cgcgattgtt tgaccgtttc tatcgcgttg acccctcccg ccagcgaaaa ggtgaaggta
     5161 gcggtattgg gctggcgata gtgaaatcga ttgttgtcgc gcataaaggc acggttgcgg
     5221 taacgtcaga tgcgcggggg acaaggtttg ttatcacatt acccgcttaa ttattcagca
     5281 aacggcaact tttataacca gtgtaaaaat aacgtgccgc aatagtcata catattaatg
     5341 gtaaaaagct gtcacaattc ataaaaaacc ttaatatacg ccaccctaaa cataaccagc
     5401 gttaatgtaa ggtttttgtg tggactggct tcttgatgtt tttgctacct ggctctacgg
     5461 cttaaaagta atcgcgataa cgttagcggt catcatgttc atcagcgggc tggacgattt
     5521 ttttattgat gtcgtctact gggtacgccg cattaaacgc aagttgagtg tttatcgccg
     5581 ctacccgcga atgagttacc gcgaactgta taaaccagat gaaaaaccgt tagcgattat
     5641 ggttccggcg tggaatgaaa cgggcgtcat cggcaatatg gccgagctgg cggcgaccac
     5701 gctcgactac gaaaactatc atatctttgt tggcacctac cccaacgacc ccgatactca
     5761 acgtgatgtt gacgaagtgt gcgctcgctt tccgaacgtg cataaggtag tctgcgcgcg
     5821 tcctggtccc accagcaaag ccgactgcct gaacaacgtg ctggacgcca tcacccagtt
     5881 tgaacgtagc gccaatttcg cttttgctgg ttttattctg catgacgccg aagatgtgat
     5941 ttcaccgatg gaattgcgtc tgttcaacta tctggtcgag cgtaaagatc tgattcagat
     6001 cccagtgtat ccgtttgaac gcgaatggac gcacttcacc agcatgactt acattgatga
     6061 gttttcagaa ctgcatggca aagatgttcc ggtgcgtgaa gccctcgccg gacaagtgcc
     6121 cagcgcaggc gtcggcacct gtttcagccg ccgcgccgtg accgcactgt tagctgacgg
     6181 taacggtatt gctttcgacg tgcagagtct tactgaagat tacgacattg gcttccgcct
     6241 gaaagaaaaa ggtatgacgg aaatttttgt ccgttttccg gtggtggacg aagccaaaga
     6301 acgcgagcag cgtaaatttt tacagcacgc acggacgtca aacatgatct gcgtgcgcga
     6361 atatttcccc gatacctttt cgactgcggt tcgacaaaaa tctcgctgga tcatcggcat
     6421 tgttttccag ggctttaaaa cccataaatg gacctccagc ctgacgctga actactttct
     6481 ctggcgcgac cgcaaagggg caatcagtaa ctttgtcagc ttcctcgcga tgctggtgat
     6541 gatccagctt ttgctgttgc tggcgtatga aagtttgtgg cccgatgcct ggcatttcct
     6601 ttctattttt agcggcagcg catggttaat gaccctgctg tggctaaact ttggcttgat
     6661 ggttaaccgc atcgtgcagc gagtgatttt cgtcactggc tactacggcc tcacgcaggg
     6721 cctgctttcc gtcctgcgtc ttttctgggg caacctgatt aactttatgg ctaactggcg
     6781 cgtgctgaaa caggttcttc agcacggcga tccgcgtcgc gtcgcgtggg ataaaacaac
     6841 gcatgacttc cccagcgtca ctggcgatac ccgctcgttg cgcccgttag gtcaaattct
     6901 gctggaaaat caggtcatca ctgaagaaca actcgataca gcactgcgta atcgcgtcga
     6961 aggtctacgc ctgggcggtt caatgctgat gcaggggctg attagcgccg agcagctggc
     7021 acaggcgctg gcagagcaaa acggcgtggc gtgggaatcc atcgatgcct ggcagatccc
     7081 gtcctcgctg attgccgaaa tgccagcctc cgtagcgctg cattatgcag tactgccgct
     7141 gcgtctggaa aatgatgagt taattgtcgg cagtgaagat ggtattgacc cggtttcgct
     7201 ggcggccctg acgcgtaaag tcggacgcaa agtgcgttac gtcattgttc tgcggggaca
     7261 aattgtcaca gggttacgcc actggtatgc acgccgacgc ggtcacgatc cgcgggcaat
     7321 gttgtacaat gcggttcagc atcagtggct cacggaacag cagaccggtg aaatctggcg
     7381 gcaatatgtg ccgcatcagt tcctgttcgc cgaaatactg accacgctcg gtcatattaa
     7441 tcgttcagca attaacgtgt tgttattgcg ccatgaacgc agttctctgc cgctcggcaa
     7501 gtttttggtc accgaaggcg ttatcagcca ggaaacgttg gatcgcgtcc tgacaattca
     7561 acgcgaatta caagtttcga tgcaatcact attactcaaa gcaggtttaa acacagaaca
     7621 ggttgcgcaa ctggagtccg aaaatgaagg agaataacct taatcgcgtc atcggatggt
     7681 ctggtttact gctgacgtct ttattgagta ccagcgcact cgcagacaat atcggcacca
     7741 gcgcagaaga gctggggctg agcgattatc gccattttgt tatttatccc cgtctcgata
     7801 aggcgctgaa ggcacagaaa aataacgacg aagcaaccgc catccgcgaa tttgaatata
     7861 tacaccaaca ggtgccggat aatattccgc tgactttata ccttgcggaa gcctatcgtc
     7921 attttggtca tgatgaccgg gcgcggctgt tgcttgagga tcaactgaaa cgtcacccag
     7981 gagatgcccg acttgagcgc agtctggcgg ctattccagt tgaagtgaaa agcgttacga
     8041 ctgttgaaga actgcttgcc cagcaaaaag cgggcgacgc tgcgccgacc ctgcgttgtc
     8101 gcagtgaagt cgggcagaat gccctgcggc tggcacaatt acctgtcgcc agagcgcaac
     8161 tgaacgatgc gacgtttgct gcatcgccgg aaggaaaaac gctgcgaacc gatctgctgc
     8221 aacgggcaat ctacctgaaa caatgatccc aggcagatac gctatacaat gaagcacgcc
     8281 agcagaacac attaagcgcg gcagaacgcc gtcagtggtt tgacgtgctt cttgccgggc
     8341 agctggacga tcggatcctg gcactgcaat cacaggggat cttcaccgat cctcagtcat
     8401 atattactta cgcgaccgcg ctggcttatc gtggcgaaaa agcacgcctc cagcattatc
     8461 tcattgaaaa taagccgctg tttaccacgg gcgcacaaga gaaaagttgg ctctatctgt
     8521 tatctaaata cagcgccaac cccgttcagg cattggcgaa ttatacggtg cagtttgccg
     8581 ataaccgcca gtatgttgtt ggcgcgacgc taccggtgct gttaaaagaa ggtcagtacg
     8641 acgcagcgca aaaactgctc gccaccctcc ccgccaatga aatgcttgag gagcgttatg
     8701 ctgtcagcgt ggcgacccgt aacaaggctg aatctctgcg tctggcacga ttgctgtatc
     8761 agcaagaacc gacaaatctt ccccgcctgg atcaactgac ctggcaactg atgcagaacg
     8821 agcagtcacg cgaagctgcc gatttattgc tgcaacgcta tcctttccag ggcgatgcgc
     8881 gtgtcagcca gactttaatg gcgcgactgg cgtctctgct ggaaagtcat ccttacctag
     8941 caacgccggc gaaggtggcg attttatcga aacccttacc gctggcggag caacgtcagt
     9001 ggcaaagtca gttgccgggt attgcagata attgcccggc aatagttcgc ttgctgggcg
     9061 atatgtcgcc ttcctacgat gctgccgctt ggaaccgtct ggcaaagtgt tatcgggaca
     9121 cgctacccgg tgtggcgttg tatgcatggc ttcaggccga acaacgacaa ccgagcgcct
     9181 ggcaacatcg tgcggtagcc tatcaggcgt atcaggttga ggactacgcc accgcactgg
     9241 cggcctggca gaaaatcagt cttcacgaca tgagcaatga tgatctgctc gccgccgcca
     9301 acaccgctca ggcagcggga aatggcgcag ctcgcgatcg ctggcttcaa caggcagaac
     9361 aacgtgagct gggaaacaat gctcaatact ggtggctgca tgcgcgacgt tatatcccag
     9421 ctcagccaga actcgcactg aacgatctca cgcgctcaat caatattgcc ccttctgcca
     9481 actcttacgt tgcgcgggcg acaatttatc gccaacgtca taatgttccg gccgcggtga
     9541 gtgatttgcg cgccgcgctg gaactggaac caaataatag caacacccag gcagcgcttg
     9601 gttacgcctt gtgggatagc ggtgatatcg cacagtcgcg ggaaatgctc gaacaggcgc
     9661 ataaagggct tccggacgat ccggcactga tccgacaact ggcctacgtg aaccagcgtc
     9721 tggatgacat gcctgcgacg cagcactacg cccggctcgt gattgatgac attgataatc
     9781 aggcataacc ccactgaccc cagagcaaaa tcagcaacgc ttcaatttcc gccgtttgca
     9841 tgaggaggtc ggtcgccgct ggacgttcag tttcgattct tccatcggct tgcgttccgg
     9901 cgcaatgagt accgctaaca ataatgtcgg cggtgcagcg cctgggaaaa gctatcgtag
     9961 ctacggacaa ctggaagccg agtaccgcat cggacgcaat atgctgctgg aaggcgacct
    10021 gctctcagtt tatagccgcg tctttgccga taccggagaa aacggggtga tgatgccggt
    10081 gaaaaatccg atgtccggca ccggtctgcg ctggaagccg ctgcgcgatc agatcttttt
    10141 cctcgccgtc gaacagcagt tgccgctgaa cgggcaaaat ggcgcatccg ataccatgct
    10201 gcgcgccagc gcctcattct ttaatggcgg caaatacagc gacgaatggc acccgaacgg
    10261 ttcaggctgg tttgcccaaa acctgtacct cgatgcggcg caatatatcc gccaggatat
    10321 tcaggcgtgg acggcagatt atcgcgtcag ctggcatcag aaggtagcta acggacagac
    10381 tattgagcct tacgctcacg ttcaggacaa cggctatcgt gataaaggca ctcagggcgc
    10441 gcagcttggc ggtgtcgggg tccgctggaa tatttggacc ggcgagacgc actacgacgc
    10501 ctggccgcac aaagtcagtc tcggcgtcga gtatcaacat acctttaagg cgattaatca
    10561 acgtaacgga gagcgcaaca acgcgtttct caccattgga gtgcactggt aaatgcgtaa
    10621 gttcattttc gtattgctga cactgctttt ggtcagccct ttttcctttg cgatgaaagg
    10681 tattatctgg caaccacaaa accgagatag tcaggttacc gatacccagt ggcaggggct
    10741 gatgagtcag ttacgttttc aaggcttcga taccctggtt ttgcaatgga cccgttacgg
    10801 cgatgcattt acccagccag aacagcgcac gttattgttt aagcgtgccg cagctgcgca
    10861 acagactggc ctgaagctga ttgtcgggct gaacgccgat ccggaatttt ttatgcacca
    10921 gaaacagtcg tctgcagcgc tggaaagcta tcttaatcgc ctgctggctg ccgatctcca
    10981 gcaagccaga ttatggagcg ccgcgcctgg cataacgccg gatggctggt acatcagcgc
    11041 ggaaattgac gacctgaact ggcgcagcga agccgcccgt caacctttgc taacatggtt
    11101 aaacaacgcg cagcggctga ttagcgatgt ttcagcaaaa ccggtttata tcagtagttt
    11161 tttcgccgga aacatgtcgc ccgatggcta tcgccaactg ctggaacaag ttaaagcaac
    11221 cggagttaat gtctgggtac aggatggcag cggcgtggat aaacttaccg ctgaacagcg
    11281 tgaacgttat ctacaggcca gcgccgattg ccaaagctcc gccccagcaa gcggcattgt
    11341 ttatgaactg tttgtcgccg gcaaaggcaa aacctttaca gcgaaaccga aaccggacgc
    11401 agaaattgcc tcgctgttag cgaaacgttc ctcttgcggt aaagacatgc tctatttctc
    11461 tctgcgctat ttgcccgtcg cgcacggcat tctcgagtat taaatcccct ccaggtaagt
    11521 cgggtacgac ctggcttacc tctttcgctc tcgtcagaat tccatcaaga ttaattgcta
    11581 aaaagcatgc taaagaaaga agatcgttat cggcgaacgg gccgatcact catgcaggaa
    11641 gcatagatcc tttgagcgga gtttctgata tctgaggagt gcgaaatgca attgagcagc
    11701 agtgaacctt gcgtggtgat cctgaccgaa aaagaggtag aggtaagcgt caataaccat
    11761 gctacgttta cccttccgaa aaactacctg gccgccttcg cgtgcaacaa taacgtcatt
    11821 gaactctcaa cgttaaatca cgtattaatc acccacatca accgtaacat catcaacgat
    11881 tatctgttgt ttttaaataa gaaattaacc tgtgtaaagc cctggtcgcg gctagcaacc
    11941 ccggttatcg cttgtcatag ccgtacaccg gaagtgttcc ggctagccac caaccacagc
    12001 aagcagcaat ccagcaaacc ctgcgaagcg gagttgacgc gcgcattact ttttaccgta
    12061 ttgtctaact ttcttgagca atcgcggttt attgccctac tgatgtatat cttacgcagc
    12121 agcgtccgcg acagcgtctg ccgcattatt caaagcgata ttcagcatta ctggaatctg
    12181 cgaattgtcg ccagttcgct atgtttaagc cccagcctgc tcaaaaagaa attaaaaaac
    12241 gaaaatacca gctatagcca gattgtcaca gagtgtcgta tgcgttacgc cgtacagatg
    12301 ttattgatgg ataacaaaaa tatcactcag gtggcgcaat tatgtggcta tagcagcacg
    12361 tcgtacttta tctctgtttt taaggcgttt tacagcctga caccgttgaa ttatctcgcc
    12421 aaacagcgac aaaaagtgat gtggtgaagg gcaaagcgga aacggataag acgggcataa
    12481 atgaagaaga aatggcgcgc cctgcaggat tcgaacctgc ggcccacgac ttagaaggtc
    12541 gttgctctat ccaactgagc taagggcgcg ttgataccgc aatgcggtgt aatcgcgtga
    12601 attatacggt caacccttgc tgagtcaatg gcttttgatc tggttgctga acaagtgaac
    12661 gaccgcgtct gattttctga tttatttcgc tatagcggca aacaaacgca caccgctgcg
    12721 cgtctgaatc aagaaaaccc gtattttcat gtatcaaagt acaatttacc gccctaacgg
    12781 aaaattgtcc gctcctatga gactggtaac tatgaaacca acgtcggtga tcattatgga
    12841 tactcatcct atcatcagaa tgtctattga agttctgttg caaaaaaaca gtgaattgca
    12901 gattgtcctg aaaacggatg attaccgcat aaccatcgat tatctccgaa cccgtcctgt
    12961 tgatttaatc attatggata tagacttgcc cggaacagac ggttttacct tcctg
//
LOCUS       DAEBXN010000110        12907 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.110, whole genome shotgun sequence.
ACCESSION   DAEBXN010000110 DAEBXN010000000
VERSION     DAEBXN010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12907
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.110"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(128..1579)
                     /gene="uxaB"
                     /locus_tag="KJL99_003325"
     CDS             complement(128..1579)
                     /gene="uxaB"
                     /locus_tag="KJL99_003325"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HBD4437931.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(1806..3224)
                     /locus_tag="KJL99_003326"
     CDS             complement(1806..3224)
                     /locus_tag="KJL99_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBD4437932.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIAFIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINLLMKQVALRADFDFLTQVYSRSGLYEVLKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(3378..3722)
                     /locus_tag="KJL99_003327"
     CDS             complement(3378..3722)
                     /locus_tag="KJL99_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HBD4437933.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVV"
     gene            complement(3722..4648)
                     /gene="glsB"
                     /locus_tag="KJL99_003328"
     CDS             complement(3722..4648)
                     /gene="glsB"
                     /locus_tag="KJL99_003328"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HBD4437934.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINTGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(4712..5647)
                     /locus_tag="KJL99_003329"
     CDS             complement(4712..5647)
                     /locus_tag="KJL99_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001580861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HBD4437935.1"
                     /translation="MRGAVPIILAGNGYLLKHAPNVMGCAQLIAQVFKDAGIPQGVYG
                     WLNADNDGVSQMIKDSRIAAVTVTGSVRAGAALKKCVLELGGSDPFIVLNDADLELAV
                     KAAVAGRYQNTGQVCAAAKRFIIEEGIASAFTERFVSAAAALKMGDPRDEENALGPMA
                     RFDLRDELHHQVEKTLAQGARLLLGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGP
                     VAAITIAKDAEHALELANDSEFGLSATIFTTDETQARQMAARLECGGVFINGYCASDA
                     RVAFGGVKKSGFGRELSHFGLHEFCNIQTVWKDRI"
     gene            5727..6842
                     /gene="yneK"
                     /locus_tag="KJL99_003330"
     CDS             5727..6842
                     /gene="yneK"
                     /locus_tag="KJL99_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HBD4437936.1"
                     /translation="MVTPVSICNYISLPDDFPARNIAPQVKEVLKDFIDTLSTIICDE
                     EWRTSLNTNSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFMNDFIEQF
                     PMINLGAPIKRAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVQPEVCICFLSLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLKLFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATAKTPDLPERH"
     gene            6992..8182
                     /gene="ydeA"
                     /locus_tag="KJL99_003331"
     CDS             6992..8182
                     /gene="ydeA"
                     /locus_tag="KJL99_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HBD4437937.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(8207..8872)
                     /gene="marC"
                     /locus_tag="KJL99_003332"
     CDS             complement(8207..8872)
                     /gene="marC"
                     /locus_tag="KJL99_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HBD4437938.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            9084..9518
                     /gene="marR"
                     /locus_tag="KJL99_003333"
     CDS             9084..9518
                     /gene="marR"
                     /locus_tag="KJL99_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HBD4437939.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            9538..9921
                     /gene="marA"
                     /locus_tag="KJL99_003334"
     CDS             9538..9921
                     /gene="marA"
                     /locus_tag="KJL99_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HBD4437940.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            9953..10171
                     /gene="marB"
                     /locus_tag="KJL99_003335"
     CDS             9953..10171
                     /gene="marB"
                     /locus_tag="KJL99_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HBD4437941.1"
                     /translation="MKPLSSAIAAALILFSAQSVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(10202..11101)
                     /gene="eamA"
                     /locus_tag="KJL99_003336"
     CDS             complement(10202..11101)
                     /gene="eamA"
                     /locus_tag="KJL99_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HBD4437942.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVVSLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            11365..12483
                     /gene="ydeE"
                     /locus_tag="KJL99_003337"
     CDS             11365..12483
                     /gene="ydeE"
                     /locus_tag="KJL99_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HBD4437943.1"
                     /translation="MATLPFMTIYLSRQYSLSVDLIGYAMTIALTIGVVFSLGFGILA
                     DKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLFFALINCAYSVFATVLKAWFADNL
                     SSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSINLPFWLAAICSAFPMLFIQIWVK
                     RSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLASFVSGAFASCISQYVMVIADGDF
                     AEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRALMTAGTLCFVIGLVGFIFSGNNLL
                     LWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKASYFSAQSLGWLGAAINPLVSGVVL
                     TSLPPSSLFVILALVIIAAWVLMLKGIRARPWGQPALC"
     gene            12610..12705
                     /gene="mgtS"
                     /locus_tag="KJL99_003338"
     CDS             12610..12705
                     /gene="mgtS"
                     /locus_tag="KJL99_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HBD4437944.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
BASE COUNT         3402 a         3299 c         2948 g         3258 t
ORIGIN      
        1 gtcaagctta caacattaac aggcgctgca tgtgacacct gttaatgata aaggaatata
       61 ggatgttgta actaacatag tgaaataaga aacccggtcg aaaccgggtt cagaagtaac
      121 ggtgttatta gcacaacgga cgtacagctt cgcgcatccc tttttcaaga atcgcatcca
      181 ggtcattagc aacctgctcg accagaccag gcacttgcgt caggtcctgc tcccagtggt
      241 ctttttctgc caacacaatc gctaccagtt cctgggtacc gataacacgg tcacgatgct
      301 ggctccagag ttgctgataa cgttccagcc agtgtgcatc gtcctgtacc ggatatgttt
      361 ctccgttacg ctcaccacga tagaacgcaa tcaatgccgc taatgcgaaa gtaaggcgcg
      421 ccggaagtgt gccatttgcc ttctgccctg ccagcagctg cggcaggatg cgggtgcgga
      481 acttggtcat accgttgagc gcgatagaca gcagctgatg cttaatgtac gggttacgga
      541 aacgcccggt gactgcactg gcgaaagatt ccagttcatc acgaggcaaa tccagtaccg
      601 gaataatttc ttcgtagatg gctttttcaa cgaatgcgca aatttcagca tcgttcatcg
      661 cttcacctac ggtatccagc cctgcctgga acgccaccgg caccagcgcg gtgtgcgcac
      721 cgttgaggat tgccactttg cgttctttat acggtttaat atcgtcaaca atcagcacgt
      781 tgagcggata tttgtccagg cgcagctcag tcgctaagga tttcggcccc tgaatcacaa
      841 acaggtaaaa gtgttcagcg gtgtcgagga aaccatcgtg ataacccagt tcttcttcca
      901 gtttagccac ttcatcgcgc ggataaccgg taacgatacg gtccaccagc gtagaacaga
      961 agctgttagc ctgatccagc cattgaataa atgcttctgg cagtgcccac tcttgcgcat
     1021 agcgcagcac cagttcacgc aacgcgtcgc cattgtagtc aatcaactca cacggaatga
     1081 tgatccaacc tttatccagc gcaccgttga aatggctaaa gcgttcgaac agcagacggg
     1141 tcagttttgc cggatagctt accgctggcg cgtcatcgaa tttatcgccc gcatggtagc
     1201 tgatacccgc ttcggtggtg ttagagaaaa caaagcgcat ttccgggttg tgcgccagtt
     1261 tcaggaattc atcgtattca ctgtagacgc tgatttcacg attaaccgag cgaatcagac
     1321 gcgcgtcgct gaccgcttcc cccttctcat tcaggccacg gataatggtg gtgtacagac
     1381 catcctgcgt gctcagtgac ggcgggaatg aagtttcaat cggacgaaca acgaccacgc
     1441 cagaattcag atcggtgtgc tcattcagga gatcgatttg ccagtcaaca aaggcgcgca
     1501 ggaagttacc ttcaccaaac tgaatgatac gttctggata ctgtgcaccg ggaaaatcgc
     1561 gacgatttag tgttttcaca atgggttccc ttctgattag tcatacaacc cgtttgaatt
     1621 ggtgcgacag gttagcaaac tttaatacgc cgaacccctg ttttgatcaa ctcctgatga
     1681 ttaatgagca gttttatgag aaaagtgtgg cgcggatcat ggtttaatcg aggaaatacg
     1741 ccttttcctg gatcataaag tggtagaaca cattgcattc aaatcgcgcg gaatgaataa
     1801 agatgtcaga caacttcctc accgtaacgc atagtgctgg tacggttgcg cccatctttc
     1861 ttcgagcgat acagacacgt atcggcttcg accatcaatt tattaaaatc atcggtcagc
     1921 gttcggtacg atgcgcgacc actaccgacg ccaatactta ccgtgagata aagcgttttt
     1981 tgttgccagg tgaatggttg cagttcaacg cctttacgga ttttttccgc cattagcaga
     2041 ccatcgacag gattcaccga cggcactgca acagcaaatt cttcgccgcc catgcgcgcc
     2101 accagcccct tatcaccgac aatcttctga atatgccggg caaacacgct taacacttta
     2161 tcgccacatt catgtccata gttatcgtta atgcttttga aatagtcgat atcaagcagc
     2221 atgaccgtca gatgttgtgt ctgtttcagc gatggacttt tcagcacctc ataaagaccg
     2281 gaccgtgagt aaacctgagt cagaaaatca aagtcggctc gcagcgcaac ttgcttcatt
     2341 agcaaattga tcgctgccac gctaaaagaa accataattg ggcatatcgc catcgtggca
     2401 ataccgagac gtgcggagaa catttccgga atggagaacg gcgaaccgac cgagatatca
     2461 atcaccgaat ttgccaccag tacgatttcc accgcaccgg tgacaaaggt caacagacat
     2521 gttacctgcg gcgtatagcg cactgcacac cagattaatg caggcagcgg gaacgccaga
     2581 ctccccgctc cgccaatgac taccgaggca ataaccgaca caataaacgc gatggcgggc
     2641 atcatctgtt ctgctttaaa gcgcggcagc actccaggaa tagccaacgt cagcatacaa
     2701 ggcacgatca acacgcccgt tgagaattgc tcactgaacc agtccgcaag caaaggccag
     2761 aaatccagac tatcaatact gaccgaacca atcgccccga caatagcgca taataatgcg
     2821 gcaatcagac agtaattaaa tagccgtaaa gcacttactg gctcatactt atttttcccc
     2881 agacgcttat cccgcacgac cagtaaggcg acggtaacaa taaacatcat attggagaaa
     2941 ttgataacca gtgaaaccag tccccattcg gtggttatgg catcataaac cagcatcgcc
     3001 acataactga tcgcatagta atgcagtcga ttaagccaga catagcgggc aaacaccccc
     3061 gccattacgc cattcagcgg ccagaaaaga gaaagttcgt gaaccagccg taactctgca
     3121 ccaataaaat agaacagcgt ggttagcgca aaaatagcga tgctattacg tagcagatga
     3181 ccttcctgga acaaacggaa agttgaaatg gggtgtacat gcattaatta tctgtccata
     3241 caattgcggg tggcaattat ctcaacgtga taagtaaatt ctcataaagg atagcatgtt
     3301 cgctaacagc ggcggcaaat aaaggtaccc tgggaatgct attttatcgt tttccgcaat
     3361 tctcatggct gattcatcta tacaaccagc cagtgataga ttaccgccac gatataacgt
     3421 tgttgttctt cgcttaacga aacaccctgc ggaatgttat gccacttagc cagacaaccg
     3481 cgacagcagg tcgcggtggc gtgctgagcg ataaacaccg gatgcccgcg catgggggtt
     3541 tgcttaccgt cattagccgg taatgccgga gcaagacgtt tagcgacaaa atccgccgca
     3601 tgttgttcga tgactggcgc gcctttctcc aggcaatact gacgctcttt catgcccaga
     3661 cgaaagcgag agcgaaattt tgaacgggat aaacgcgcga agagtggatc gagagactgc
     3721 attaataaac cgaacgccct aactgtttcg tcaattgttc aaggacggcg atacccgcaa
     3781 gcgagttacc tgcatcatcc agttccggac tccagactgc gatggccatt tcatgcggaa
     3841 caatcgccac aataccgcca ccaacgccag atttcgccgg tagtcccacc cgccaggcaa
     3901 actcccccgc gttctggtac ataccactgg tcgccatcag cgcgttaatt tgccgcgcct
     3961 gcattggcgt caccacaggt tcatcaatat gaatagcttt cccctgatta gccagaaaga
     4021 caaacgtccg ggccagctct acacagctca ttttcagagc gcagtaatga aagtagtttt
     4081 gcagaacggt tgtcacgtca tgatggaaat tgccaaacga cttcatcagc caggcgatag
     4141 ccgcatttcg cgcggaatgt tcaaattcgg aacgcgctac caccgtatcg tatgaaatat
     4201 cagacacacc gcttaagccg cgcacgactt ccagcatacg ttgccgtggt gcgcttagtc
     4261 gcccttgcaa catatcgcag accaccagcg caccggtatt aatgaacgga ttacgcggaa
     4321 taccctgttc catttccagt tgcactaaag aattgaacgg tgatccagac ggatctttgc
     4381 cgacgcgttg ccagatttcc tcttcggagt aatgacgcat ggcgacaacg agactcagca
     4441 ctttggaaat agactgaatg gaaaaacgtt cttgcgcgtc tccggcctga aaaagctgtc
     4501 cgtcaacggt acagatagca atccccaatc gggaaccgtc tactgtagcc agcgccggaa
     4561 tataatccgc gactttaccc tgaccaatga gcggccgcac ttgccgcaag atgttttcta
     4621 aaattgcatt atccatggcg actgccactt tctactcctg gaccgcaggt ctgaaaagac
     4681 ctgcgagtat atcagagctg aatatgtcgc gtcagatccg gtctttccac actgtctgga
     4741 tattacagaa ttcgtgtaag ccgaaatggg aaagctcacg accaaagcca ctctttttca
     4801 cgccaccaaa ggccactcgc gcgtcgctgg cacaataacc attgataaac accccaccgc
     4861 attccagacg tgccgccatc tgtctggcct gtgtttcgtc ggtggtaaaa atggtcgctg
     4921 aaaggccgaa ctcactatca ttagccagtt ccagcgcatg ttctgcatct ttcgcaatgg
     4981 tgattgcagc aacggggcca aacatttctt cccgaaacgc ggtcatttct ggggtaacat
     5041 tcgccagaac cgttggcgga tagtagttac ctgctccagc catcttttcc ccgcccagta
     5101 acaaacgcgc accctgcgcc agggttttct ccacctgatg atgcagctca tcacgtaaat
     5161 caaaacgagc cattggtccg agagcgttct cttcatcacg gggatcgccc attttcaagg
     5221 ctgccgcagc tgacacaaaa cgttcggtaa atgccgaagc aattccctct tcgataataa
     5281 agcgttttgc cgctgcacaa acctgtccgg tattctgata acgtccggct accgccgctt
     5341 taaccgccag ttccagatcg gcatcgttaa gcacaataaa tggatccgaa ccgcccagtt
     5401 ccagtacgca ttttttcagt gccgctccag cacgaacact tccggtcacc gtgacagcag
     5461 caatgcgcga gtctttaatc atctgactga caccgtcgtt gtcggcattc agccagccat
     5521 atacgccttg cgggattccc gcatctttaa acacttgggc aatgagctga gcacagccca
     5581 tcacattcgg cgcatgttta agtaagtagc cgttacctgc aagaatgatg ggaacagcgc
     5641 cacgcatcac ctgcctttga tagttcttgc agctcaaatg ccctatatag tgatgttccg
     5701 atgacttatg actatatggg gcaaatatgg ttacgccagt aagcatctgc aattacatat
     5761 ctcttcctga tgattttccc gctcgtaata ttgcacctca agtaaaagaa gttttaaagg
     5821 attttattga tacactcagt accataatat gtgatgaaga gtggcgcacg tctttaaaca
     5881 ccaactcagc cacaaaaaag atatttaaca accttgataa cttatcatat attcagagga
     5941 cttcttttcg gggtaacgac acactataca atgaaaaggt tcagtttaaa ctcacttatc
     6001 ctgtcaagaa tggaagacac aaagaaaata ttgaatttca ggtagtaata aatttaagcc
     6061 ctatttattt agataatttt cgccatgatg gagaaattaa tattttttgc gctcccaacc
     6121 caaagcctgt cactatgggg cgcgtatttc agaccggcgt cgagcgtgtg ctctttctgt
     6181 ttatgaatga ttttattgaa caatttccaa tgatcaacct tggcgccccc atcaaaagag
     6241 cgcatacacc acatattgaa cccctgcctc cggatcacca taccgctgca gattacttac
     6301 gccagtttga tttgcttgtc ctgaatttta tctctcgcgg taattttgtc atactccccc
     6361 gattatggaa taactctgag gttcacagat ggttcgtcaa taaagatcct aacttgatca
     6421 ccgccattct cgacataacg gacagcgaat taaaagagga tttattgcaa agcctgatgg
     6481 attcattagg ttctaacaaa catgtacaac ccgaagtctg catctgcttc ttatcccttt
     6541 tagcagaaca agaatctcct cattttcagg acttgttttt atttttcgcc aatatgttat
     6601 tgcactatca ccaatttatg aatcccaatg aaagtgatct gaatgacgtg ttgatgccag
     6661 catcattaag tgatgataaa attatcaaac atatggcgcg caggaccctc aaactgtttg
     6721 taaaaaatga aacgccgcca aaagttactc acgaagacct ggtgaaaaac aggcctcgct
     6781 cccctgtcag accacctata cccgcaaccg ccaaaacgcc agacctccct gaacgtcatt
     6841 aaaccgtgat gttaccgact ctctgactcg gaacagaatc agcgtcagag aaacggaaaa
     6901 cgcgatccag atcacaaatg cattgtattc acatcattaa ccgttttaag atcatttcat
     6961 cactttttcg caactcaccc gataatctgt tatgacaaca aacactgttt cccgcaaagt
     7021 ggcgtggcta cgggtcgtta cgctggcagt cgccgccttc atcttcaaca ccaccgaatt
     7081 tgtccctgtt ggcctgctct ctgacattgc gcaaagtttt cacatgcaaa ccgctcaggt
     7141 cggcatcatg ttgaccattt acgcatgggt agtagcgcta atgtcattgc cttttatgtt
     7201 aatgaccagt caggttgaac ggcgcaaatt actgatctgc ctgtttgtgg tgtttattgc
     7261 cagccacgta ctgtcgtttt tatcgtggag ctttaccgtt ctggtgatca gtcgcattgg
     7321 tgtggctttt gcacatgcga ttttctggtc gattacggcg tctctggcga tccgtatggc
     7381 tccggccggg aagcgggcac aggccttgag tttaattgcc accggtacag cactggcgat
     7441 ggtcttaggt ttaccgctcg ggcgcattgt ggggcagtat ttcggctggc gaatgacctt
     7501 cttcgcgatt ggtattgggg cgcttgttac ccttttgtgc ctgattaagt tacttccctt
     7561 actgcctagt gagcattccg gttcattgaa aagcctcccg ctattgttcc gccgcccggc
     7621 attgatgagc atttatttgt taactgtggt ggttgtcacc gcccattaca cggcatacag
     7681 ctatatcgag ccttttgtgc aaaacattgc gggattcagc gccaactttg ccacggcatt
     7741 actgttatta ctcggtggtg cgggcattat tggcagcgtg attttcggta aactgggtaa
     7801 tcaatatgcg tctgcgttgg taagtacggc gattgcgctg ttgctggtgt gtctggcact
     7861 gctgctacct gcggcgaaca gtgaaataca cctcggggtg ctgagtattt tctgggggat
     7921 cgcgatgatg atcatcgggc ttggtatgca ggttaaagtg ctggcgctgg caccagatgc
     7981 taccgacgtc gcgatggcgc tattctccgg catatttaat attggaatcg gggcgggtgc
     8041 gttggtaggt aatcaggtga gtttgcactg gtcaatgtcg atgattggtt atgtgggcgc
     8101 ggtgcctgct tttgctgcgt taatatggtc aatcattata tttcgccgct ggccagtgac
     8161 actcgaagaa cagacgcaat agttgaaagg cccattcggg ccttttttaa tggtacgttt
     8221 taatgatttc caggatgccg ttaataataa actgcacacc catacatacc agcaggaacc
     8281 ccatcaggcg ggagatcgct tcaatgccac ccttgcccac cagccgcata attgcgccgg
     8341 agctgcgtag gcttccccac aaaataaccg ccaccaggaa aaagatcagc ggcggcgcaa
     8401 ccatcagtac ccaatcagcg aaggttgaac tctgacgcac tgtggacgcc gagctaataa
     8461 tcatcgctat ggttcccgga ccggcggtac ttggcattgc cagtggtaca aaggcaatat
     8521 tggcactggg ttcatcttcc agctcttccg acttgctttt cgcctccggt gaatcaatcg
     8581 ctttctgttg cggaaaaagc atccgaaaac cgataaacgc gacgattaag ccgcctgcaa
     8641 ttcgcagacc gggaatcgaa atgccaaatg tatccatcac cagttgccca gcgtaatacg
     8701 ccaccatcat gatggcaaat acgtacaccg aggccatcaa cgactgacga ttacgttcgg
     8761 cactgttcat gttgcctgcc aggccaagaa atagcgcgac agttgttaat gggttagcta
     8821 acggcagcaa caccaccagc cccaggccaa ttgctttaaa caaatctaac attggtggtt
     8881 gttatcctgt gtatctgggt tatcagcgaa aagtataagg ggtaaacaag gataaagtgt
     8941 cactctttag ctaaccttgc atcgcattga ccaaaacttg aaccgattta gcaaaacgtg
     9001 gcatcggtca attcattcat ttgacttata cttgcctggg caatattacc ccctacaact
     9061 aattacttgc caaggcaact aatgtgaaaa gtaccagcga tctgttcaat gaaattattc
     9121 cattgggtcg cttaatccat atggttaatc agaagaaaga tcgtctgctt aacgagtatc
     9181 tgtctccgct ggatattacc gcggcacagt ttaaggtgct ctgctctatc cgctgcgcgg
     9241 cgtgtattac tccggttgaa ctgaaaaaag tgttgtcggt cgacctggga gcactgaccc
     9301 gtatgctgga tcgcctggtc tgtaaaggct gggttgaaag gttgccgaac ccgaatgata
     9361 agcgcggcgt actggtaaaa cttaccacca gcggcgcggc aatatgcgaa caatgccatc
     9421 aattagttgg ccaggacctg catcaagaat taacaaaaaa cctgacggcg gacgaagttg
     9481 caacacttga gcatttgctt aagaaagtcc tgccgtaaac aaaaaagagg tatgacgatg
     9541 tccagacgca atactgacgc tattaccatt catagcattt tggactggat cgaggacaac
     9601 ctggaatcgc cactgtcact ggagaaagtg tcagagcgtt cgggttactc caaatggcac
     9661 ctgcaacgga tgtttaaaaa agaaaccggt cattcattag gccaatacat ccgcagccgt
     9721 aagatgacgg aaatcgcgca aaagctgaag gaaagtaacg agccgatact ctatctggca
     9781 gaacgatatg gcttcgagtc gcaacaaact ctgacccgaa ccttcaaaaa ttactttgat
     9841 gttccgccgc ataaataccg gatgaccaat atgcagggcg aatcgcgctt tttacatcca
     9901 ttaaatcatt acaacagcta gttgataacg tgacaacgtc actgaggcaa tcatgaaacc
     9961 actttcatcc gcaatagcag ctgcgcttat tctcttttcc gcgcagagcg ttgcggaaca
    10021 aaccacgcag ccagttgtta cttcttgtgc caatgtcgtg gttgttcccc catcgcagga
    10081 acaaccaccg tttgatttaa atcacatggg tactggcagt gataagtcgg atgcgctcgg
    10141 cgtgccctat tataatcaac acgctatgta gtttgttctg gccccgacat ctcggggctt
    10201 attaacttcc cacctttacc gctttacgcc accgcaagcc aaatacattg atatacagcc
    10261 cggtcataat gagcaccgca cctaaaaatt gcagacccgt taagcgttca tccaacaata
    10321 gtgccgcact tgccagtccg actacgggca ccagtaacga taacggtgca acccgccagg
    10381 tttcatagcg tcccagtaac gtcccccaga tcccataacc aacaattgtc gccacaaacg
    10441 ccagatacat cagcgacaag atggtggtca tatcaatagt aaccagactg tgaatcatgg
    10501 atgcggaacc atcgagaatc agcgagacaa caaagaaggg aatgattggg attaaagcgc
    10561 tccagattac cagcgacatc accgccggac gcgttgagtg cgacatgatc tttttattga
    10621 agatgttgcc acacgcccaa ctaaatgctg cagccagggt caacataaag ccgagcatcg
    10681 ccacatgctg accgttcaga ctatcttcga ttaacaccag tacgccaaaa atcgctaagg
    10741 cgatccccgc caattgtttg ccatgcagtc gctccccgaa agtaaacgcg ccaagcacga
    10801 tagtaaaaaa cgcctgtgcc tgtaacacca gcgaagccag tccagcaggc ataccaaagt
    10861 taatggcgca gaacagaaag gcaaactgcg caaaactgat ggttaatcca taccccaaga
    10921 gcaaattcag tggtactttc ggtcgtgcaa caaaaaagat ggccggaaaa gcgaccagca
    10981 taaagcgcaa accggccagc atcagcggtg gcatgttatg aagccccact ttgatgacca
    11041 caaaatttag cccccatacg accactacca gtagcgccaa caccccatct tttcgcgaca
    11101 ttctaccgcc tctgaatttc atcttttgta agcaatcaac ttagctgaat ttacttttct
    11161 ttaacagttg attcgttagt cgccggttac gacggcatta atgcgcaaat aagtcgctat
    11221 acttcggatt tttgccatgc gtccgatggt taataacaac gacgaggcaa gaagggcgct
    11281 ggtagagcgt cgtagggata agttcatttg tctgtccggc agtgcgtttc gttatgtttt
    11341 attttagaga tgtaaagaaa tagcatggct acgctgccat ttatgaccat ttacttgagt
    11401 cgccagtaca gcctgagtgt cgatctaatc ggttatgcga tgacaattgc gctcactatt
    11461 ggcgtcgttt ttagcctcgg ttttggtatc ctggcggata agttcgacaa gaaacgctat
    11521 atgttactgg caattaccgc cttcgccagc ggttttattg ccattccttt agtgaataac
    11581 gtgacgctgg ttgtgctctt ttttgccctc attaactgcg cctattctgt ttttgctacc
    11641 gtgctgaaag cctggtttgc cgacaatctt tcgtccacca gcaaaacgaa aatcttctca
    11701 atcaactaca ccatgctaaa cattggctgg accatcggtc cgccgctcgg cacgctgttg
    11761 gtgatgcaga gcatcaatct gcccttttgg ctggcagcta tctgttccgc gtttcccatg
    11821 cttttcattc aaatatgggt aaagcgcagc gagaaaatca tcgccacgga aacaggcagt
    11881 gtctggtcgc cgaaagtttt attacaagat aaagcactgt tgtggtttac ctgctctggt
    11941 tttctggctt cttttgtaag cggcgcattt gcttcatgca tttcacaata tgtgatggtg
    12001 attgctgatg gggattttgc cgaaaaggtg gtcgcggttg ttcttccggt gaatgctgcc
    12061 atggtggtta cgttgcaata ttccgtgggc cgccgactta acccagctaa catccgcgcg
    12121 ctgatgacag caggcaccct ctgtttcgtc atcggactgg tcggttttat tttttccggc
    12181 aacaacctgc tattgtgggg tatgtcagct gcggtattta ctgtcggtga aatcatttat
    12241 gcgccgggcg agtatatgtt gattgaccat attgcgccgc caggaatgaa agccagctat
    12301 ttttccgccc agtctttagg ctggcttggt gccgcgatta acccattagt gagtggcgta
    12361 gtgctaacca gcctgccgcc ttcctcgctg tttgtcatct tagcgttggt gatcattgct
    12421 gcgtgggtgc tgatgttaaa agggattcgt gcaagaccgt gggggcagcc cgcgctttgt
    12481 tgatttaagt cgaacacaat aaaaatttaa ttcagccttc gtttaggtta tctctgctaa
    12541 tatctttctc attgagatga aaattaaggt aagcgaggaa acacaccaca ccataaacgg
    12601 aggcaaataa tgctgggtaa tatgaatgtt tttatggccg tactgggaat aattttattt
    12661 tctggttttc tggccgcgta tttcagccac aaatgggatg actaatgaac ggagataatc
    12721 cctcacctaa ccggcccctt gttacagttg tgtacaaggg gcctgatttt tatgacggcg
    12781 aaaaaaaccg ccagtaaacc ggcggtgaat gcttgcatgg atagatttgt gttttgcttt
    12841 tacgctaaca ggcattttcc tgcactgata acggatcgtt gacacagtag catcagtttt
    12901 ctcaatg
//
LOCUS       DAEBXN010000111        12835 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.111, whole genome shotgun sequence.
ACCESSION   DAEBXN010000111 DAEBXN010000000
VERSION     DAEBXN010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12835
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.111"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..414
                     /gene="torD"
                     /locus_tag="KJL99_003339"
     CDS             <1..414
                     /gene="torD"
                     /locus_tag="KJL99_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HBD4437945.1"
                     /translation="TLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQEIKR
                     LLVEAGMETSGNFNEPADHLAIYLELLSYLHFSLGEGTVPARRIDSLRQKTLTALRQW
                     LPEFAARCRQYDSFGFYAALSQLLLVLVECDHQNR"
     gene            complement(507..812)
                     /gene="cbpM"
                     /locus_tag="KJL99_003340"
     CDS             complement(507..812)
                     /gene="cbpM"
                     /locus_tag="KJL99_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HBD4437946.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(812..1732)
                     /gene="cbpA"
                     /locus_tag="KJL99_003341"
     CDS             complement(812..1732)
                     /gene="cbpA"
                     /locus_tag="KJL99_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HBD4437947.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            1993..3249
                     /locus_tag="KJL99_003342"
     CDS             1993..3249
                     /locus_tag="KJL99_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HBD4437948.1"
                     /translation="MGSNIHGISCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSG
                     GITYTIQYVQDVDIVRVSLPGRGSESITDFKGYYWYGFMEYIENINACDDVFSEYCLD
                     NENMSIQPEQINMPGISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAIL
                     VLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFYNLREDVKTLFGVDLQS
                     DNKIQGVGSFMSWSFLFFKKQFIDSCLNDKKCILHLPEFIFNDNKNLLALDTDTSDRI
                     KAVKNFLAVLSDSICSLFIVNSNVASISLGNESFSTDEDLEYGYLTNTGNHYDVYLPP
                     ELFSQAYKLKNKEMNAQLDYLNRYAT"
     gene            3542..4783
                     /gene="agp"
                     /locus_tag="KJL99_003343"
     CDS             3542..4783
                     /gene="agp"
                     /locus_tag="KJL99_003343"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HBD4437949.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGIVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPMITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRASAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(4821..5048)
                     /locus_tag="KJL99_003344"
     CDS             complement(4821..5048)
                     /locus_tag="KJL99_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HBD4437950.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(5069..5665)
                     /gene="wrbA"
                     /locus_tag="KJL99_003345"
     CDS             complement(5069..5665)
                     /gene="wrbA"
                     /locus_tag="KJL99_003345"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HBD4437951.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            6038..6145
                     /locus_tag="KJL99_003346"
     CDS             6038..6145
                     /locus_tag="KJL99_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4437952.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
     gene            complement(6227..7555)
                     /gene="rutG"
                     /locus_tag="KJL99_003347"
     CDS             complement(6227..7555)
                     /gene="rutG"
                     /locus_tag="KJL99_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HBD4437953.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSYAAWFGLPHFSTPGFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNANLIMVAVTF
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(7576..8070)
                     /gene="rutF"
                     /locus_tag="KJL99_003348"
     CDS             complement(7576..8070)
                     /gene="rutF"
                     /locus_tag="KJL99_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HBD4437954.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            complement(8081..8671)
                     /locus_tag="KJL99_003349"
     CDS             complement(8081..8671)
                     /locus_tag="KJL99_003349"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HBD4437955.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLQELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            complement(8681..9481)
                     /gene="rutD"
                     /locus_tag="KJL99_003350"
     CDS             complement(8681..9481)
                     /gene="rutD"
                     /locus_tag="KJL99_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HBD4437956.1"
                     /translation="MKLSLSSPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTMLVSVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            complement(9489..9875)
                     /gene="rutC"
                     /locus_tag="KJL99_003351"
     CDS             complement(9489..9875)
                     /gene="rutC"
                     /locus_tag="KJL99_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HBD4437957.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFYIQCGLVKPDALVEIATIAHIAK"
     gene            complement(9887..10579)
                     /gene="rutB"
                     /locus_tag="KJL99_003352"
     CDS             complement(9887..10579)
                     /gene="rutB"
                     /locus_tag="KJL99_003352"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HBD4437958.1"
                     /translation="MTTLTARPEAITFDPQQSAQIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEHFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            complement(10579..11670)
                     /gene="rutA"
                     /locus_tag="KJL99_003353"
     CDS             complement(10579..11670)
                     /gene="rutA"
                     /locus_tag="KJL99_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HBD4437959.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIAQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSIPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQATEQTGRDVGSYVLFMVIADETDDATRAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLSALTQEVA"
     gene            11958..12596
                     /gene="rutR"
                     /locus_tag="KJL99_003354"
     CDS             11958..12596
                     /gene="rutR"
                     /locus_tag="KJL99_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HBD4437960.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
BASE COUNT         3276 a         3227 c         3266 g         3066 t
ORIGIN      
        1 acgctgacag tacgtgacga tgcccgtctg gaactggccg ctgacttttg cggcctgttt
       61 ctgatgaccg acaaacaagc ggcgctgccg tatgcatcgg cctacaaaca ggacgagcaa
      121 gagattaaac gcttgttagt tgaggcaggg atggaaacca gtggcaattt caacgaaccg
      181 gcagatcatc tggcgatcta tctcgagttg ttgagttatc tgcatttttc gctgggagag
      241 gggaccgttc ctgcgcgaag aatcgacagt ttgcggcaaa aaacactgac ggcgctgcgg
      301 caatggttac cagagtttgc tgcgcgttgt cgacagtatg acagctttgg tttttacgcg
      361 gcactaagcc agttattgct ggtgttagtg gagtgcgacc accaaaacag ataacgtcgt
      421 ttatgcgcct gaaaagacgc gtttagcgtc gcatcaggca ttatggcgca gttgccggat
      481 gcgtcatcac atccggcaaa cacacatcac ggatgagcta caaaccggga aagccgctgg
      541 cgtagcaggc ggttttcctg cttcaggtgc gcaatatcat ccattaacgt cagcgccacc
      601 gcgatccccg gccagtccag agccagttca tgacgcaggc gtaccgcgcg ttgcaccaca
      661 atggcggcat ggtcgtcaaa tacccaggtt gtttcctgga tctcacgcgg ttcaaccacc
      721 cccaaaccga caatttcatt caactcctct tcagagatgc cggtatgcag gcaaaattcg
      781 gtaatagtaa aagtcaccgt aacattagcc attatgcttt cccccaatct ttacgtggat
      841 caaaagacga ctgggcgtct gccagttgct gccacagcgc ggcagtgttt tcatccggtt
      901 tcggcggcat cacgattttc agtaccgcat acagatcgcc ggtctgtttt ttgctcacca
      961 gacctttgcc tttaacgcgc aatcgttgcc cggcctggct gcctggcggg atagtcagca
     1021 aaatgctttc tttcagtgtt ggaacggtga ctttagtacc cagcgccgct tcccacgggc
     1081 taaccggcac cacaatttcc agatcctggc cgacaatatc gaacagcgga tgtggcgcaa
     1141 tatgaatcac cagccacaaa tcgccatttg gaccgccgtt ttcgcccggc gtcccctggc
     1201 ctttcaggcg gatgcgttga ccattgccga cgcctgccgg gatcttcaca ttcagcgttt
     1261 tcgggatttc ctgttcgatc atgccaaagg cgttataaac cggcaggtta tagctgatgg
     1321 tacgcttatg ctcagtaagc gtttcttcga ggaataccgc cacttcgatt tcaatatcgt
     1381 ggccacgtgc ggcggggcgt tgacggctct ggcgggcatg ctgaccgaaa attgacgaga
     1441 agatatcgtc aaaatcttcg gcattaaaac tctgaccgtc gccatgatgg aactgacggt
     1501 taaattgcgg atcgttgcga tgttgccaca tctgatcata ctcagcgcga cgttgttcat
     1561 cacttaacac ttcccaggct tcagcgacct ctttgaagcg ggcttcggca tccggttctt
     1621 tgctgacatc aggatggtat ttacgggcca gtcgacgata ggcggtcttg attgtcttga
     1681 gatcgtccgt cggtttcacg cccatgatgg cgtaataatc ctttaattcc atagcgttat
     1741 ctcgcgtaaa tcaacacaaa ttgaaggaac ccctgtaagg taactcctat aagtgtaggg
     1801 taatcctcaa aatttcatat gccaacacag aatatgttat tgaaatcatc gcggagagga
     1861 ggtcgccatc aagatgggtt gctgaacata ttttaaacag gtgaaaaagg gtgagcgatt
     1921 tttgatagtt gaaccaggca ctttaagttt aactagggcg tcattattta ttaaatttta
     1981 tagacgctat atatgggtag taatatacat ggaattagtt gcactgcaaa taattatttg
     2041 aaacaggcct ggaacgatat aaaaaatgag tacgaaaaaa atcaaacata ttcaatcacg
     2101 ctttttgaaa acacactggt gtgttttatg cggttgtaca atgaactcag acgtaaagta
     2161 aatgaagagg atactccatg tctggaatgt gaatcactag aaaaagaatt tgaggaaatg
     2221 cagaatgata atgatctatc attatttatg agaacattgc gtactaatga tacacaaatt
     2281 tattcagggg tttcaggagg tattacatat actatacaat atgttcaaga tgttgatatt
     2341 gttagagtgt ccttgccggg cagaggttca gagtctatca cagattttaa aggttattat
     2401 tggtatggct ttatggaata tattgaaaac attaatgcgt gtgatgatgt tttttctgag
     2461 tattgtttag ataatgaaaa tatgagtatc cagccagaac agataaatat gccgggaata
     2521 tctgatttag atactggtat tgatttgtct ggcatatctt ttattcagag tgaaattaat
     2581 aaaacatatg gattaaaata cgctccagta gatggcgatg gatattgtct gttaagagcc
     2641 atactggttt taaaagaaca tgagtattca tgggcgctgg gcagccataa gacgcaaaag
     2701 caagtatacg aagagtttat taaaattgtt gataaacaaa cgatcgaggc tcttgttgat
     2761 acggcattct ataatctcag ggaagatgta aagacattat ttggcgttga tctgcaatct
     2821 gacaacaaaa ttcagggtgt gggtagtttt atgtcatgga gctttctgtt ttttaaaaaa
     2881 caattcattg atagttgctt gaatgacaaa aaatgcatac tgcatttacc cgagtttata
     2941 tttaatgata acaagaattt gcttgcttta gataccgaca cgtcggatag gattaaagcg
     3001 gtgaagaatt ttcttgctgt tctttcagat agcatttgct cattatttat tgttaatagt
     3061 aatgtggcat caatctcctt gggaaatgaa tccttttcaa cagatgaaga tcttgagtat
     3121 ggatatttaa caaacactgg caatcattat gatgtttacc tccctcctga acttttttct
     3181 caggcttata agttaaaaaa taaggaaatg aatgcgcaac ttgactattt aaatcgttat
     3241 gcaacttaat ggcaaaggca tatgctaaaa accattgtta ttagtctcac acttttttat
     3301 tggtaaatat tgtctctgta ttggtaacgc cgcagatatt ctgtttagcc acaggtgcaa
     3361 ttatcagcgg cgtacgcgag gcaggggcta atcaggcata gtttgcgtca aaccttgcct
     3421 gtttttgaag atgtatatag aaaaacaggc gttcaacaag ccattttgcg aacctgttcc
     3481 cggaaaaaag tcatatttct gtcacactct ttaatgattg ataacaaaag aggtgccagg
     3541 aatgaacaaa acgctaatcg ccgcaactgt ggcagggata gttttactcg cttcaaatgc
     3601 tcaggcacaa accgtaccgg aaggctatca gctacagcaa gtgctcatga tgagccgcca
     3661 taacttacgt gcgccgctgg cgaacaatgg cagtgtgctg gagcagtcga cgccgaataa
     3721 atggcccgaa tgggacgtcc ccggtgggca actcaccacc aaaggtggcg tgctcgaagt
     3781 gtatatgggc cattacatgc gtgaatggct ggcagagcag gggatagtga aatcggggga
     3841 atgcccgccg ccggacaccg tttatgccta tgccaatagt ctgcaacgta ccgtcgctac
     3901 cgcccagttc tttattactg gtgcattccc tgggtgtgat attcctgtgc atcaccagga
     3961 aaaaatgggc actatggacc caacctttaa cccgatgatc accgatgatt ccgccgcatt
     4021 cagtgaacag gcggtggcgg caatggagaa agagctcagc aaactccagc ttaccgacag
     4081 ctaccagcta ctggaaaaaa tcgttaacta taaagattcc cctgcctgta aagagaaaca
     4141 acagtgttcg ctggtggatg gcaaaaatac ctttagcgcc aagtatcaac aagaaccagg
     4201 tgtttccggg ccgctgaaag tcggcaactc gctggtagat gcgtttactt tgcaatacta
     4261 cgaaggtttt ccgatggatc aggtggcctg gggagaaatc aaatctgacc agcagtggaa
     4321 ggtattgtcg aagctgaaaa acggttacca ggacagtctg tttacctcac cggaagtggc
     4381 gcgcaatgtt gcgaaaccgc tggtcagtta tatcgacaaa gccctggtca ccgatcgcgc
     4441 cagtgctccg aaaatcacgg tgttagttgg gcacgactcc aacatagcct ctctgttaac
     4501 ggcgctggat ttcaaaccgt atcagttgca tgaccagaac gaacgcacgc cgattggcgg
     4561 caaaatcgtt ttccagcgtt ggcatgacag caaagccaat cgcgatttga tgaaaattga
     4621 atatgtgtac cagagtgcgg aacagttacg taatgccgat gcgttaaccc tgcaggcacc
     4681 tgcgcagcgc gtgacgctgg aattaagcgg ttgcccgata gatgccaacg gtttctgccc
     4741 gatggataag tttgatagcg tgttgaatga ggcggtgaaa taacagaaaa ctcccccgcg
     4801 agaagcgggg gagtcgctgg ttaaacgttt ttacgttcga tggtctgttc gccccaaaaa
     4861 agcgaatctt tatcggtctt agcaaaggct ttgactaaca cttcatcact accttcttcc
     4921 caaatctttt ctgccatttt ttcgtcgtac ccggcgactt cgaaaatggc ctcggctatt
     4981 tccggcgacg tattgcgcag agatgcccat tcaccgacgt gatgagcttt cgcttcttga
     5041 gttggcatgc gtatcctcct gttgaagatt agccgttaag tttaactgcc agacctgcga
     5101 catattcccc ttgataacga gcaatagaca gttcttcctg gcttggctgg cgtgagccgt
     5161 caccgcctgc gatggtggtt gcgccgtacg gcgtaccgcc gcgaacctgt gaaacgtcaa
     5221 ataattcctg cgctgcgtag ccaatgggga caatcaccat gccgtgatgc gcaagggtcg
     5281 tccaggtgga tgtgatggtt tgttcctgac cgccgccagt accggtggaa ctaaagacgc
     5341 tcgccagttt tccgtatagt gcgccggaag cccacaggcc gcccgtctgg tcgaggaagg
     5401 tacgcatttg accggacatg ttgccaaagc gggtaggtgt accaaaaata atggcgtcgt
     5461 aatcggccag ttcttgcggg gttgcaaccg gtgcagtttg cgttttaccg cctgcttttt
     5521 caaataattg cggcggcatg gtttccggta cacgcttaac gacaacttct gcgccatcca
     5581 ctttgcttgc accctcagcg actgcgcgtg ccatcgtttc aatatgtccg tacatggaat
     5641 aataaagcac cagaacttta gccatttcta accactcctc gtgttatctc tattccgtag
     5701 cgattcgcta ccacttattt aaagataaga cgtccttttc agagtgcaaa tttcacaacc
     5761 acttatttga tttataacaa ctttcacaag cacgtaattt tgtcgcaaaa tgacacattt
     5821 ttatctcatc gcgttttttt aatcataaga gcggcttatg gataattatt ggagatgata
     5881 tctattctcg ctaagaagct gttgcaggat attaccaaac gcgggtctgt ccgcgtcagt
     5941 tcactaagct tagtcccacg tagcgaaaat atggcagccg ccatacgccg cgttaattct
     6001 atgcaatatg atgtctatac ccagacggag gtcagtaatg gcaaaccatc gaggcggttc
     6061 cggcaatttt gcagaagacc gcgaaagagc atcagaagca ggtaaaaaag gtggcaagag
     6121 cagtcacggc aaaagcgaca actagccggg ctaatcaatg acgaatgcat ttttttctta
     6181 agctcgtcaa aaagtcatca ccgccggtaa cccggcggtt ttctgtttat ggctcctgat
     6241 gaacaacttc tggcggtgga acgtcaacca accttctgct taataacgca ttgagtaaaa
     6301 tcgcgccaaa ggttgctgta ccgatccctc ccaacgtaaa accgccaagc gtgagagcaa
     6361 aatcacccgc gcccagcacg aaggttactg cgaccataat caaattagcg ttctggctta
     6421 aatcgacacg gttttgtacc catatccttg cgcctgcgac ggcaatcagc ccgaacacaa
     6481 caattgatgc accaccaatc accgcggccg gaatggtatg gatcagcgcg ccaaatttcg
     6541 gcgaaaagcc caacagcata gcgatgacgg cagctgcaac aaacaccagc gtcgagtaga
     6601 cttttgtcac ggccatcaca ccgatatttt cagcataggt ggtgacgccg ctaccgccga
     6661 cagagccgga aagcatcgtt gccagaccat caccgacgaa tgctcgcccc atatacgggt
     6721 ccatattgcg tccggtcatc ccggcgaccg ccttgagatg acctaagttt tccgccacca
     6781 gaatcaccgc cacgggcgca atcaacatca tcgcttggcc attaaaacca ggagtggaaa
     6841 aatgcggcag accgaaccag gcagcatagc tgacgagagt aaaatcgacg gcttttccca
     6901 gccctaaaac gttggtcatc acgccataca gcagacaggc gacaattaat cctacgagaa
     6961 tcaataaccg ctggatcatg ccacgggtaa acaccgccac cagcccaata cacagcaccg
     7021 tcattaccgc catccagcta tcaaaggccg aagccgatac acttttcact gcgataggcg
     7081 ctaagttcag gccaatcgcc atcaccaccg cacccgtcac caccggcggc attagtcgtt
     7141 caatccagcg tgtaccaatt ttcatcacca ccaggccaat gacggtataa accagcccac
     7201 aggcgataat cccgcccagc gcgatgctga tattcgggtt aatgccctga ccgttaaagc
     7261 ccgtcgcggc aatcaccacg ccgacaaaag ccgcgctgga gccgagataa ctggggacgc
     7321 gcccgccggt aataaagaaa aacagtaacg tgccgatccc cgacattaaa atggaaagat
     7381 tgggatccag ccccatcaga atcggcatta acaccgtcgc gccaaacatc gccaccgcgt
     7441 gttgaacgcc cattactgcc gtctgagcaa acggcaatcg ttcatccggc gcgaccacgc
     7501 cgctctctgt agaggtcgat tttaactgcc agtgaggaaa accgaacatt gccatcagct
     7561 gtctccttaa ggaggttaac aagcagggcg catcagcgcg tgataactgc gatcgaacca
     7621 caccagcccg tagggtgtgg tgtgacgatg aatcgcttcg atggcgcaaa acagaatgtc
     7681 gtgggtgccg acgctcacca cctggctgat acggcagtca aacgaaacca gagcctcttc
     7741 cagttgcggg catccggtca cacccgtctg ccagcgggcg gcggcaaagc ggtgttccat
     7801 gggcgttttg ccgccaaaaa gatttgaaag cggctcctgc ccggcgctca gtgtatttac
     7861 acacagcgtt cgattttcat tgaataccgg ccagacggac gccccacgat tcaggcacac
     7921 cagtaatgtg ggcggcgtgt cggtcacgct gcagacggcg ctggcggtga acccggcgcg
     7981 cccggctgga ccgtccgtgg tgatgatatt gaccgccgcg cccatacagg acatcgcatc
     8041 gcgaaaggtt tgttgatcaa ctatgttcat agtttgctcc ttacaacagc ccgcaggctt
     8101 cttcaaagga cagacgtggc aggcgcgcaa aaagtttgct gctatcgcca tagccgatat
     8161 taatcagcag attgctcttc agcgtgctgc ccgcaaaaaa ggcgtcgtca acgtgttgac
     8221 ggtcaaaacc cgacatcggg ccggtatcca gtcccagcgc acggcaagcg acgatcagat
     8281 aagccgcctg catggaactg ttgcgaaacg ctgtttcttc ggcaagttgt gggctggagg
     8341 taaaccaact gcgggcatca ccgtggggaa acagtagtgg taaccgttca taaaattcac
     8401 tgtcccaggc gacgatagcg gtgacgggcg cggtcagggt tttttgcaga ttgccgctgg
     8461 aaagcgccgg gcgcagacgt tcttttcctt ctgcagtgcg gataaacacg atccgtgccg
     8521 gagaacagtt agctgatgtc ggcccccatt tcatcagggc ataaagctcc tgtaacgtct
     8581 catcgctgac gggtgtctcc cgccagccgt tgtgagtgcg ggcatcggtg aacagggtgc
     8641 taagcgcacc tgggctaacg gcttcgttca tagcaattcc ttacagggcg gcttcacggt
     8701 gatgtaacag gctggcaagc ccgttgagta acagagcatt aaacgtttcg ggatcggtca
     8761 cgttgcaggc gtgtccgcca tagggcatca ccattttctg gctatcgggc agggcggcat
     8821 gaagttcact ggaacatgct gttggcacca gcagatcatc actggcgcag atgatttgca
     8881 ccgggcagcg gatgcgatcc gcatggtgac taaagtcagc gcgtttgagg gcgttaagtc
     8941 gacgtagtaa attatttttg ccctgaaaat gcgccagtgc cagcgcgtct tctgcctcca
     9001 ggcgaggtgc gcgggccgcc atccagtcgg cgggatagag gaacaacggc tgcgcttcca
     9061 cccatgcttg cgcgccgccg ctatacagta atcgttcgcg aacctgaaaa cagcggcgcg
     9121 tatgggcgtt tattcgtagc cagccgttaa cgctgaccag catagttacc gacgcgggat
     9181 aatccagcgc cagctgcatc cccaccagcg caccgagcgc atggccgacc accgcgtaac
     9241 gctcaatccc tgcggctacc agcgcctgat gcagttccgc tgccatctgg gcgatactgt
     9301 aatcttctgc cagtgtgtcg ggattattgc cggtgccgcg ctggtcgtaa cagactacct
     9361 gatactcctg ctccagcacc gccagttgcg gtaaccagta actgccgcta ccgccaagac
     9421 ccgaaatcaa caccactacg ggtgcatcag cataaggggg agatgagagt gaaagtttca
     9481 tcgcggcctc acttggcgat atgcgcaatt gtggcgattt ccaccagcgc gtcaggtttt
     9541 accagtccgc actgaatgta gaatcgcgcc ggtttatcac ccggaaaaaa ctcggcgtag
     9601 atttcgttaa tcgcggcgta atttttccag tcggtaataa agatgctgtt gaaggtcaca
     9661 tccgccatcg tgccacccgc cgtctcgatc accttgcgga tagtttccag aacgtggcgg
     9721 gtttgcgcct ttgggtcatc ggcaaacagc acgttattat gttgatcaaa agccagcgta
     9781 ccggagacat acaccacgcc atcagccagc gtgccgggaa cgaagggggc cagcggtgcg
     9841 ctgctgccag cgggaataat tacggatttt ggcatcgcta aactccttaa gcgatacgag
     9901 caaaggacgt gggagaaagc gcgtcgcaga acgtttcgac gtcgctgacc cagccaaaaa
     9961 aggtttcgat attgaacaac gcggctttct gtgcaaattc cggccccgcc tggtgtgttg
    10021 cgtcttccag caccacgccg aaatgctcca gaaaaaagcc atcgcgtagc gtcgattcga
    10081 cgcagacgtt ggtagcgata ccggtgaaaa ccagatggcg tattccacgg ctgcgtaaaa
    10141 tgctgtccag cggcgtattg aagaaaccgc tgtagcgcgg cttcggcagc acaatatcgc
    10201 caggctgcgg caccagttca tccaccagtt gataatccca ggagcctttc gccagcaatt
    10261 tcccctgcag ctgcggctgc ttacgcatgg ttttcagggc gttcgattta tgaaaattcg
    10321 gtgagccggg gccgccagcc tcgacatact gttcatccca gccattttga aaccagatga
    10381 tcaacatccc tgccgctcgc gctgcggtca cagcggtttg aatgttggca atgaccgggc
    10441 gagtggttga gacatcaaac ccggcgagat ctaagtagcc gcctggcgtg gcataagcgt
    10501 tttgcatatc caccacgatc tgcgcacttt gctgcggatc gaaggtaatg gcttccggtc
    10561 gagcggttaa ggttgtcatc atgccacctc ctgagtcagc gcagagagat gggcgcggca
    10621 ctgcatcagt ggttgaatgc gctcgccgaa ggtttcgatt cccgacagaa aatcatcgaa
    10681 ggtcaacagc acgccttctg caccaggcac gcttgcgact tcatctaaca tgcgcgcgac
    10741 actggcgtaa gaaccgacta acgtccccat attgatgttt accgccgaag tgggatcggc
    10801 catctgacga acgttggtgt cagtaccgga gcgggtatct ttctgacttt gttcggttag
    10861 ccagcttaac gcctcttcat ccgcgcccgc tttgtagtgt tcccatttgg cgcgagtggc
    10921 atcgtcggtt tcatcggcaa tcaccataaa caacacataa gagccaacgt cgcgtccggt
    10981 ttgctctgtg gcctgtttca tccgcgcagc ggtcggggcg aaagccgtgg gtgtatttac
    11041 gcctttgccg aaacagaagt tgaaatcggc atactgggcg gagaacgcca tgccagcgtc
    11101 gctttgcccg gcgcagatca ctttcatggg gatactcggt tgcggactga cgcgacaatc
    11161 attcatggtg aaaaaatcgc ctttaaaatc gcttttcccc gtgccccaca ggtcgcgcag
    11221 cacctgaaca tattcggtga gatagtcgta acgacgggag aaatagtcat cgccaggcca
    11281 gatacccatc tgctcatact cgggcttttg ccagccagtc acgaggttga cgccaaaacg
    11341 cccgccagag atggagtcga tggttgcggc catacgggcg acgattgccg gaggtaacgt
    11401 taaggtggca gcagtggcgt aaatctgaat gcgcgaggtc acggctgcca gacccgccat
    11461 caaggtgaac gactcaaggt tgtgatccca gaactcagtt ttgccgccaa agccacgcag
    11521 tttgatcatc gacagggcga aatcgaaatg gtaatgctcc gctttttgcg caatggcttt
    11581 attcagttca aaggtcggca tgtactgcgg cgcgtgggtc gaaatgagcc agccgttgtt
    11641 gccaataggt acgaatacgc caattttcat catcaacctc tcttcgtctc gtaaagtgaa
    11701 agtcagacgg ggcgctgcat cctgcatatc cttttcagcc gcgtattggc ttgttcgcaa
    11761 agcggatgcc agtttttaaa aagtgaatat tattaatgtg ttagcattgt gttatctaaa
    11821 atatctggta aaaagtggac taaacggtca aaacagttgc acataaaaca tgcatccgtg
    11881 cgcgatgaga gtgcagaagg tcgaggccgg gcgggggttt tgctatcctg ttaccaatct
    11941 acaagagggg agagcgcatg acgcaaggcg cagtgaaaac aacgggtaaa cgttcgcgcg
    12001 cagtaagcgc gaagaaaaaa gcgattctta gcgcagcact ggacactttt tcacaattcg
    12061 gttttcacgg cacaaggctg gagcagatcg cagagttggc gggtgtttca aaaaccaatc
    12121 tgctgtatta cttcccgtca aaagaggcgc tgtatattgc cgtgctgcgg cagattctcg
    12181 atatctggct ggcaccgtta aaagcgtttc gtgaagattt cgccccgctg gcggcgataa
    12241 aagagtacat ccgtctgaag ctggaagtct cacgcgatta tccgcaggct tcgcgcctgt
    12301 tctgtatgga gatgctggca ggcgcgccgt tgttaatgga tgaactgacg ggcgatttga
    12361 aggcattaat tgatgagaaa tcggcgctga ttgccggttg ggtcaaaagc ggcaaactcg
    12421 cgccgattga tccgcagcat ttgattttta tgatttgggc ttccactcaa cattacgccg
    12481 atttcgcccc tcaggtggag gcggtgacag gcgcgacgtt gcgcgatgag gtatttttca
    12541 atcaaacggt tgaaaacgtg cagcggatta ttattgaggg gattcgacca cgttaaagat
    12601 gacggaggag gttgtaacat cctccggcta cctgtttaac cgatcgtcat taaactggcg
    12661 ttaccgccag cggcagcggt attcacactc agcgaacgtt catcgatagg aattaaaacc
    12721 ccaaaaagat taaaaaaaca ccacaaaacg gatgtttctt caacaccact tttgctccat
    12781 atgaacggaa ccgacgatta aactggatgg ctctgattga ttcagggtat gaatg
//
LOCUS       DAEBXN010000112        12734 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.112, whole genome shotgun sequence.
ACCESSION   DAEBXN010000112 DAEBXN010000000
VERSION     DAEBXN010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12734
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.112"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..1039
                     /locus_tag="KJL99_003355"
     CDS             <1..1039
                     /locus_tag="KJL99_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBD4437961.1"
                     /translation="FFPNLVDHLLVKHHTADGVEDGISICYFKPYSQGRARWQGDTIH
                     GVWFDEEPPYSIYGEGLTRTNKYGQFSILTFTPLMGMSDVVTKFLKNPSKSQKVVNMT
                     IYDAEHYTDEQKEQIIASYPEHEREARARGIPTMGSGRIFQIPEETIKCQPFECPDHF
                     YVIGGMDFGWDHPQAQVQLWWDKEADTIYVSRVWKAKEKTAVQAWGAVKSWAHKVPTA
                     WPHDGNQHEKGGGEQLKGQYADAGFMMLQEHATWPDGGNAVEPGITELRDMMLDGRFK
                     VFNTCEPFFEEFRLYHRDENGKIVKLNDDVLSAVRYAYMMRRFAKMMRDIKKPKEKKI
                     PAPIRPIARRT"
     gene            1040..3205
                     /locus_tag="KJL99_003356"
     CDS             1040..3205
                     /locus_tag="KJL99_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HBD4437962.1"
                     /translation="MADENRLNSILCKFDADWMASDEARTEATNDLYFSRVSQWDDWL
                     SNYTTLQYRGQFDVVRPEVRKLVAEMRRNPVDVLFRPKDGANPDAADVLMGMYRTDMR
                     HNTAKIAVNVGVREQIESGVGAWRLVTQYEDNDPTSNNQVIRRLPIHEACSHVIWDAN
                     SKQMDKSDAKHCTVINALSRNGWKEFAEDYGIDPDTLPSFQNPNDTWLFPWVSNDVVY
                     VAEYYEVEEEKEKVFIYRDPLTGEPVSYYQQDIKDVIDDLANRGFIKVAERKVKRRRV
                     YKSIITCTQILKDREKIAGEHIPIVPVYGEWSFAGDKECYEGVVRLTKDGQRLRNMIM
                     SFNADIVARSPKKKPTFFPEQIEGYEYMYGGNDDYPYYLQNRTDENGNDLPIGPISYM
                     ENPEVPQANAYMLEAATNAVKEVASLGVDAQAANGQVAFDTVNQLNMRADLETYVFQD
                     NLATAMRRDGEIYASMVNDIYDVPRHVTLTLEDGSEKDVQLYAQVVDYQSGNVVTLND
                     IRGRYECYTDVGPSFQSMKEQNRAEIQELLTKVPQGTPEFQMLMLQYFTLLDGKGVEM
                     MREYANKQLVMMGLKKPETPEEMEMVQQAQQQPQQPSAEQIQAQGILLQGQAELLKAE
                     NQQAQIQVEAAKVEAQNQLNAAKIAEIFNNMDLDKQAELREYLRLVGQFQQQRSKDAR
                     ANAELLLKEADQTHSQRMDFANLMRQVQIPSGGVAETPQ"
     gene            3219..4130
                     /locus_tag="KJL99_003357"
     CDS             3219..4130
                     /locus_tag="KJL99_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein"
                     /protein_id="HBD4437963.1"
                     /translation="MDQTTDIQASEELTLPGNHAAASADGLVVDNANDSAGQEEGFEI
                     VRKDDEKPKQDPATNAEFARRRIERKRQRELEQQMEAVKRGELPEHLRVNPELPKQPD
                     PNDYLSEDALAKYDYDQSRALAAFQQANSEWQIKAMDARSQAVAEQGRKTQEFTQQSA
                     QYVEAARKHYEAAEKLNIPDYQEKEDAFMQLVPPAVGADIMRLFPEKSAALMYHLGAN
                     PEKTRQLLAMDGQSALIELTRLSERLTLKPRAKPVSEAPLPDEPIQGHAVAANISAIE
                     KQMEAAANKGDVETYRKLKAQLNKGIR"
     gene            4130..5425
                     /locus_tag="KJL99_003358"
     CDS             4130..5425
                     /locus_tag="KJL99_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coat protein"
                     /protein_id="HBD4437964.1"
                     /translation="MALNEGQLVTYALDEIIGTVQNLTPMASKVTKYPPPAESMQRSS
                     NTVWMPVEQEAPTQTGWDLTGNATGILELSVKCNMGDPDNDFFELRADDLRDERSYRR
                     RIQASAKKLANNIESAIAKQATEMGSLVVHDTRAIGPSTGLPGWDFVSDAERLMFSRE
                     LNRDMGISYFLNPDDYRKAGRNLVDGDIFGRVPEEAYRNGTIQRQIAGFDEILRSPKL
                     PAVTKSTATGVTVSGAQKFKPQAYTLDTDGNKENVDNRVATVTVSSTTGFKRGDKISF
                     IGVKFLSQMAKNVLTDDATFSITRVIDGTHIEITPKPIALDDASLTKEEKAYANVNTS
                     LADTTPVNVLNVATTTANVFWADDSIRLLSQPIPVTHELFAGMKTSSFSIPGIGVNGI
                     FATQGDINTLSGKCRIAVWYSACAVRPEAIGVGLPNQTA"
     gene            5470..5700
                     /locus_tag="KJL99_003359"
     CDS             5470..5700
                     /locus_tag="KJL99_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4437965.1"
                     /translation="MTHMIFRHGDMKKWKGVGYDFEIVKAEELQEYLDAGWFSHPDDL
                     LKDVAEPEPEEKQRKKPGRKPKAAADEPDNEG"
     gene            5678..6178
                     /locus_tag="KJL99_003360"
     CDS             5678..6178
                     /locus_tag="KJL99_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RmuC"
                     /protein_id="HBD4437966.1"
                     /translation="MNLTTKGDLVLAALRKLGVASNATLTDVEPQSMEDGVNDLEMMM
                     AEWLGGDASPGINVGYIFADADVAPDPGDEHGLSNNAINAVIFNLACRIAPDYALEAS
                     AKLITTARYGKERLVKLSAMDRAKAAKCKSGYPNRMPVGSGNQLAKWNGWNYFHRKEP
                     CDNGSE"
     gene            6178..7596
                     /locus_tag="KJL99_003361"
     CDS             6178..7596
                     /locus_tag="KJL99_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HBD4437967.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPTDSGFTQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDLEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIDSGQVSPIASASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSIANGPQWCVLKTGLY
                     DDVYRAIDFVYEGNQITCGDKLESVTGKLQFDISSQYDKQQEHLLFTPLFKADNARVF
                     DLEVESSTGVAQYADRLFLSATTDCINYGREQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFKLRVITKSPVTLSGAQIRIE"
     gene            7596..8444
                     /locus_tag="KJL99_003362"
     CDS             7596..8444
                     /locus_tag="KJL99_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4437968.1"
                     /translation="MADSNLNVPVIIQATRLDTSVLPRNIFSQSYLLYVIAQGTDVGN
                     VANKANEAGQGAYDAQVRNDEQDVILADHEQRISAAEATLVNHEERIRQAESTLQDHE
                     TRIAQNESDISSLDTRVQSLESQVSDHETRIDALEYATTRKKSEVVYSGVSVTIPTAP
                     TNLVSLLKTLTPSSGSLAPFFDTVNNKMVVFNENKTLLFKLSIVGTWPSGTANRSMQL
                     TFSGSVPDTLVSSRNAATTTDNILLATFFSVDKDGFLATNGSTLTIQSNGAAFTATTI
                     KIIAEQ"
     gene            8444..8899
                     /locus_tag="KJL99_003363"
     CDS             8444..8899
                     /locus_tag="KJL99_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HBD4437969.1"
                     /translation="MITFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQFGGIVYYQEIQPLTLDCHAMYLPEIRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            8902..9594
                     /locus_tag="KJL99_003364"
     CDS             8902..9594
                     /locus_tag="KJL99_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HBD4437970.1"
                     /translation="MLYAFKLGRKLRGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSKQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNYNNLANVGLGALQGQANAG
                     QTYANNMSSIAQQSAALAAANANKPSSLQTAISGGTSGAIAGAGLASLLGTSTPWGAG
                     IGAGIGLLGSLF"
     gene            9604..10935
                     /locus_tag="KJL99_003365"
     CDS             9604..10935
                     /locus_tag="KJL99_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HBD4437971.1"
                     /translation="MATWQGTNGGLLAGIGGVNSNAPSVNDIGNTLQLIRQNNDIERS
                     GANNVGLTALQGLSGIAGVFQQEKQAQRQKEFQQAYANAYASGDRGALRQLATQYPDQ
                     IESVRKGMGFIDEEQRNSIGTLAAGARLASSSPEAMQSWLQNNAGELARVGVNPHDVA
                     QMYQQNPRQFGEFVDHLGMNSLGPEKYFDLQDKMQGRQVTMRGQDLDSQTAARNQAIT
                     MRGQDIQANLGQQRINLDAETNRINNENKRLDRMLSAETNDLKRQEIQSRIAANNQQL
                     QQKQQALNDGYKDGINTLTTSMFTLNDIVSSPSLKSITGLRGVIPNVPGSQAADTQAR
                     LDTFKSQAYLTAVQAMRGMGALSDAEGKKLDQAVGSLQNSQSEESFRRNAGVILNTLN
                     QKRNEAVGKYVQQNGIKRVEAPQASIDYLKQHPELSIDFINRYGYLPSLGQ"
     gene            10936..12666
                     /locus_tag="KJL99_003366"
     CDS             10936..12666
                     /locus_tag="KJL99_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HBD4437972.1"
                     /translation="MANYRDLLEQAGARYGVPEGLMTALGAKESSYNPAAVSSAGAVG
                     LTQVMPGTWRDMGYTDEQMQNPEYQADAGARYLAKMYQQFGNWRDSLQAYHDGPGNVM
                     KAKRGEYTPGPEGRGYVDDRFAQWAGDPVTDSTVEQRATSAKVHPQQDPNNPFAQLEV
                     QSSEQVSASGVQSDPNNPFAQIEQQAASQQPPQPVSSVAPKPVQQQTVNQANNAPTRE
                     EPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGGPKVLD
                     DVYRPVDRPTDPYAQAGESIGGYLVPGAGVAGNMVIGSLADAANQRGDFAENAAINAG
                     LSIATHGLINGVTRGVRGASNIISGNKTSAQRATTAPTETSPFSGDAAAATNPAVHAA
                     EARVAQGVPMTPATKNPEEVVRTVAAQKRPNLASSLDELDINPQAEVLESAERLNVDS
                     LLPSHFSGNEQYKAVEQAIKSRAGSALQVQENEAIRQLAQGAGEIIDRVSGAKDALGM
                     SDKFIDTVNGRMSALMKRSDQLYRNVEKAMPAGAKIDAPSTRSMLKQVAEDLGGMKNL
                     DPIEKRVEVA"
BASE COUNT         3436 a         3144 c         3355 g         2799 t
ORIGIN      
        1 tttctttccg aaccttgttg atcaccttct ggttaagcat cacacggctg atggcgttga
       61 agatggcatt tcaatctgct acttcaagcc atactcgcaa ggccgtgctc gctggcaggg
      121 tgacacaatc cacggcgtgt ggtttgacga agagccacca tacagcattt atggcgaagg
      181 tcttacccgt accaacaaat acgggcaatt ctcaattctg acgtttaccc cgctgatggg
      241 gatgtctgac gttgttacca agttcctgaa gaaccccagc aagtcgcaga aagtggtcaa
      301 catgaccatc tatgacgctg agcactatac cgacgagcag aaagagcaaa tcatcgcatc
      361 atatcccgag catgagagag aggcgcgtgc tcgcggtatt cctacgatgg gtagtggtcg
      421 aatcttccag ataccggaag agacgattaa gtgtcagccg ttcgagtgtc ctgatcactt
      481 ctacgtaatt ggcgggatgg atttcggatg ggatcaccca caggcgcaag ttcagctttg
      541 gtgggataag gaggcagaca caatctacgt ttcacgcgtg tggaaggcga aagaaaaaac
      601 agccgttcag gcgtggggag ccgttaaatc atgggcgcat aaagtgccaa ccgcatggcc
      661 tcatgatgga aaccagcacg agaagggcgg cggtgagcag ctcaaagggc agtatgcaga
      721 cgctggattt atgatgttgc aggagcatgc gacatggcct gatggcggta acgctgttga
      781 gccaggcatc actgaattgc gcgacatgat gctcgatggt cgcttcaaag tattcaatac
      841 ctgtgagcca ttctttgagg agttccgcct ctatcaccgt gatgaaaacg ggaaaatcgt
      901 caagcttaac gacgacgtgc tatccgccgt tcgctatgca tacatgatgc gccgcttcgc
      961 aaaaatgatg cgcgacatca aaaaaccaaa agagaaaaag ataccagccc caatcaggcc
     1021 catcgcacgg agaacttaaa tggccgacga aaacagactc aattccattc tgtgtaagtt
     1081 tgacgcagac tggatggcga gcgatgaagc cagaaccgag gcgacaaatg acctgtattt
     1141 tagccgagtg tcgcaatggg atgactggct atcaaactac actaccctgc aatatcgcgg
     1201 acaattcgat gttgttcgcc cggaggtcag gaaactggtc gcagagatgc gccggaaccc
     1261 tgtcgacgtt ctcttccgac ccaaagacgg cgctaatcct gatgcagccg atgtattgat
     1321 ggggatgtat cgtactgata tgcgccataa cacggcaaag attgccgtta acgttggcgt
     1381 tcgtgagcag atagagtcag gcgttggtgc atggcgtctg gtcacgcagt acgaagacaa
     1441 cgatccaaca agcaacaatc aggtaatccg acgcctgcca atccatgaag cctgctcaca
     1501 cgtcatatgg gacgccaaca gcaagcagat ggataagagc gacgctaagc actgcacggt
     1561 gattaacgcc ttgtcgcgca atggctggaa agagttcgca gaggattacg gtattgaccc
     1621 ggataccctg ccatctttcc agaatcctaa cgatacatgg ctattcccgt gggtgtcgaa
     1681 tgatgtcgtc tacgtcgctg agtattacga ggtagaagag gagaaggaga aggtcttcat
     1741 ctaccgcgac ccgctgacag gtgagccggt cagctattac cagcaggata tcaaagacgt
     1801 catcgacgac ctggctaatc gtggattcat taaggtagca gagcgcaagg tgaagcgtcg
     1861 gcgtgtgtat aagtcgatca tcacctgtac gcagatactg aaagaccgcg agaagatagc
     1921 cggagagcat attccaatcg ttccagtgta tggcgaatgg tcattcgctg gtgacaagga
     1981 gtgctacgag ggtgtggtaa ggctgacgaa agacggtcaa cgccttcgta acatgattat
     2041 gtcgttcaac gccgatattg ttgctcggtc accgaagaag aaaccgacct tcttccctga
     2101 gcaaatcgaa ggctacgaat acatgtacgg cggaaatgat gactatccgt actatctgca
     2161 gaacaggact gatgaaaacg gtaacgacct gccgattggt ccaatctcct acatggaaaa
     2221 ccctgaagtg ccgcaagcca acgcttacat gcttgaggct gccaccaacg cagtgaaaga
     2281 agtggctagt cttggcgtgg atgcgcaggc ggcaaatggt caggtcgctt ttgataccgt
     2341 caatcaactg aacatgcggg cagaccttga gacatacgtg tttcaggata acctggctac
     2401 cgcaatgcga cgtgatggcg agatttatgc ctcaatggtc aacgatattt atgacgttcc
     2461 tcgtcatgta acgctgacac ttgaagatgg aagcgagaaa gacgttcaac tctatgcgca
     2521 agttgtcgat taccagtccg gcaatgtggt cacactcaac gacattcgcg gtcgatatga
     2581 gtgctataca gacgttgggc catccttcca gagtatgaag gaacagaacc gcgcagagat
     2641 tcaggagttg ctcaccaagg ttccgcaagg tactccagag ttccagatgc tgatgctgca
     2701 atacttcacg ctgcttgacg gtaaaggcgt cgagatgatg cgagagtacg cgaacaagca
     2761 actggtgatg atggggctga agaaaccaga aacacctgaa gagatggaga tggtgcagca
     2821 ggcacaacaa cagccgcagc agccatcagc agagcaaatt caggcgcagg gtatccttct
     2881 gcaaggtcag gctgaattgc tcaaggcaga gaaccaacag gcgcagattc aggttgaagc
     2941 tgccaaggtt gaagcccaaa accaactcaa cgccgcgaag attgcagaaa tattcaacaa
     3001 tatggacctc gacaagcagg cagaactgcg tgagtacctc aggctcgtag gtcaattcca
     3061 gcaacagcgc agcaaagatg ctcgtgctaa cgctgagctg cttcttaaag aagcagacca
     3121 gactcattca caacgcatgg atttcgcgaa tcttatgcgt caagttcaaa tcccctccgg
     3181 cggagtagcc gagacacctc aataagagag agttaatcat ggaccaaacc accgacattc
     3241 aggcttctga agaattaacc ctgcccggca atcatgcagc ggcatctgct gatggcttag
     3301 ttgtcgataa tgccaacgac agcgcaggtc aggaagaagg ctttgagatt gtccggaaag
     3361 acgatgagaa accaaaacaa gacccggcaa ctaatgctga atttgcccgt cgccgcatcg
     3421 aacgcaaacg ccagcgtgag cttgagcagc agatggaagc ggttaagcgt ggagagttgc
     3481 cggagcacct gcgggtgaac cctgagttac caaaacaacc agaccctaac gattatcttt
     3541 ccgaagatgc actggctaag tacgactatg accagagccg cgcactggct gccttccagc
     3601 aggcaaacag tgaatggcag atcaaggcta tggacgcacg aagccaggct gtcgccgagc
     3661 agggtcgcaa aactcaggag ttcacccagc aatcagcgca atacgtcgag gcggcccgta
     3721 agcactacga ggcagcggaa aagctcaata tccctgacta tcaggagaaa gaggatgcat
     3781 tcatgcaact ggtgccgcca gcagtcggtg ccgacatcat gcgcctcttc ccggagaaat
     3841 ccgccgctct catgtatcac cttggtgcta atcctgagaa aacacgccag ttgctggcga
     3901 tggacgggca atccgcgctg attgaactca ctcgactgtc agaacgttta actctcaagc
     3961 ctcgagccaa gcctgtttca gaagccccgt tacctgatga acccattcag ggacatgctg
     4021 ttgctgcaaa tatctctgcg attgaaaagc agatggaggc ggcagcaaac aaaggggatg
     4081 tagagacgta ccgcaagctc aaggcgcaac tgaataaagg aattcgataa tggcattaaa
     4141 tgaaggtcaa ctggtcacgt atgctctgga tgaaatcatc ggaaccgtcc agaacctgac
     4201 gccaatggcg tcaaaagtga caaaataccc ccctccggca gaatccatgc agcgttcaag
     4261 caacaccgtg tggatgcctg ttgagcagga agcgccaacc cagactggct gggatttaac
     4321 tggcaacgca accgggattc tggaactctc cgtgaagtgc aacatgggcg atccggataa
     4381 cgatttcttc gagcttcgtg cagatgacct gcgtgatgag cgttcttacc gtcgccgcat
     4441 ccaggcatcc gccaaaaaac tggcgaataa cattgagtca gcgattgcca aacaggcaac
     4501 tgaaatgggc tcgcttgttg ttcacgatac ccgcgcaatt ggtccatcga ctggcctgcc
     4561 tggctgggat tttgtgtctg atgcagagcg actgatgttc tcccgtgagc taaaccgcga
     4621 catgggcatc agttacttcc tgaaccctga cgattaccgc aaagcaggcc gcaacctggt
     4681 agatggtgac atcttcgggc gcgttcctga agaagcatat cgaaacggta ctattcagcg
     4741 tcagattgct ggctttgatg aaattcttcg ctcaccgaaa cttccggcag ttaccaagtc
     4801 aaccgcgact ggtgtaactg tttctggtgc gcagaagttt aagccgcagg catacaccct
     4861 tgataccgat ggtaacaaag agaacgtcga caaccgtgtt gcaacggtga ccgtatcctc
     4921 caccaccgga tttaagcgcg gcgacaaaat cagcttcatt ggtgtgaagt tcctgtctca
     4981 gatggcgaag aatgtgctga ctgatgacgc gactttctca atcacccgtg tgatcgatgg
     5041 tactcacatc gaaatcacgc cgaagccgat tgcgctggat gacgcgtcac tgacaaaaga
     5101 agagaaggct tacgctaacg taaacacctc tcttgctgat accactccgg taaacgttct
     5161 gaacgtggca acaaccaccg ctaacgtgtt ctgggctgat gactcaatcc gcctgctgtc
     5221 tcagccgatc ccggtaaccc atgaactgtt tgctggcatg aaaacgtctt ccttcagcat
     5281 tccaggtatt ggtgttaacg gcatcttcgc aacgcagggt gatatcaaca ctctgtctgg
     5341 taagtgccgt attgctgtgt ggtattcagc atgtgctgta cgaccagagg caattggtgt
     5401 tggtctgcct aaccagaccg cgtgataacc agagggagct tcggctcccg tttttatctg
     5461 gagacaagca tgacacacat gatctttcgt catggcgaca tgaagaagtg gaaaggcgtt
     5521 ggctacgact ttgaaatcgt gaaagccgaa gagcttcagg aatatctgga tgctggctgg
     5581 ttttcacatc ctgatgacct tttgaaggat gttgcagagc cagagccaga agaaaaacag
     5641 cgtaaaaagc ctggtcgaaa acctaaggcg gcagcagatg aacctgacaa cgaagggtga
     5701 tttagttctt gcggcattac gtaagctcgg tgttgcatca aatgccacgt taaccgatgt
     5761 cgaaccgcag tctatggaag acggcgtcaa cgaccttgaa atgatgatgg ctgaatggct
     5821 tggcggtgat gcgtcacctg gtatcaacgt tggctacatt tttgctgatg cagatgttgc
     5881 tccggatccg ggcgatgagc acggtttatc aaataacgct atcaatgccg tcattttcaa
     5941 ccttgcctgc cgaattgctc cagattatgc gctggaagcg tctgcaaaac ttataaccac
     6001 tgccagatac gggaaagagc gactcgtcaa actgtctgca atggacaggg caaaagcagc
     6061 taaatgtaag tccggttatc caaaccgtat gcctgttggt agtggtaacc agttggcgaa
     6121 gtggaatggt tggaattact tccaccgaaa ggaaccttgc gataacggga gcgaataatg
     6181 cctattcagc aacttccgct tatgaaaggt gtcggcaaag acttccgaaa cgccgactat
     6241 atcgactatc tgccagtgaa tatgctggct acacccaaag aaatcctgaa cagcagcgga
     6301 tatcttcgct cattcccggg cattgccaaa cgttctgacg tgaacggtgt atctcgcggc
     6361 gtcgagtaca acatggcgca gaatgctgtt tatcgcgtgt gtggcggcaa gctgtacaaa
     6421 ggagaaagtg aagtcggtga tgttgccgga agtggtcgtg tatcaatggc gcatggtcgg
     6481 acatcacagg cggtaggcgt taatggtcaa ctggtcgagt atcgctatga tggcacggtc
     6541 aaaaccgtct caaactggcc tacagacagt ggattcactc agtatgagtt aggttcagtt
     6601 cgcgacatta cgcgcttacg tgggcgttat gcgtggtcaa aagacggaac tgattcatgg
     6661 tttatcactg accttgaaga cgaatctcat cctgaccgtt acagcgcaca atatcgtgcc
     6721 gagtctcagc ctgacggcat catcggtatc gggacatggc gagacttcat cgtctgcttt
     6781 ggttcatcga ctattgaata tttctccctg actggcgcaa ccaccgttgg tgccgctttg
     6841 tatgttgcac aaccatcgct gatggtgcag aaaggcattg ccggaactta ctgcaaaacg
     6901 ccattcgctg attcgtatgc gtttatcagc aatccggcaa cgggtgcgcc gtctgtgtat
     6961 atcatcgact ccggtcaggt gtcaccaatt gccagcgcga gcattgagaa aatcctccgc
     7021 tcctacactg ctgatgaact ggctgatggt gtgatggaat cgctgcgatt tgatgctcat
     7081 gagttgctga ttatccacct tccgcgtcac gtcctcgtgt acgacgcatc ttcaatcgcc
     7141 aatggcccac aatggtgtgt gctgaaaaca ggtctgtatg acgatgtgta ccgcgctatc
     7201 gacttcgttt acgaaggcaa tcagataacg tgcggcgata agctggaatc tgttaccggg
     7261 aaactgcaat tcgatatcag cagccagtac gacaagcaac aggaacacct gctgtttact
     7321 ccactcttca aagcggataa cgccagagtg ttcgaccttg aggttgaatc gtcaactggc
     7381 gttgcgcagt acgccgaccg cctttttctc tctgcaacca ctgactgcat caattacggg
     7441 cgtgagcaga tgattgagca gaatgaaccg ttcgtttacg acaagcgtgt tttgtggaag
     7501 cgagtagggc gcatcaggaa aaatgtcggc ttcaaattgc gcgttatcac gaagtcacct
     7561 gtcactctgt ctggcgctca gataaggatt gagtaatggc tgattcgaat ctcaatgtgc
     7621 cggtaatcat ccaagctacg cggctcgata catcagtcct tccacgcaat atcttctcgc
     7681 agtcgtatct gctttacgtt atcgcacagg gcactgatgt tggtaacgtg gctaacaaag
     7741 ccaacgaggc cggacagggc gcttatgatg cacaggtcag gaacgatgag caggatgtga
     7801 ttctcgctga ccatgagcag cgaatttctg ctgcggaagc aacgcttgtt aatcatgagg
     7861 agcgaatcag acaggcagaa tcaactcttc aggaccatga aacacgaata gctcagaatg
     7921 aaagcgatat ttcgtcgctt gacacaagag ttcagtcgct ggagtcgcag gtttcagacc
     7981 atgaaacgcg catcgatgct ctggagtatg caaccacacg caagaagtca gaggttgttt
     8041 actctggtgt atctgtaacc atcccgacag cgccgaccaa ccttgttagc ctgctgaaaa
     8101 cgctcacacc gtcatccggc tcgttggcac cattcttcga caccgttaac aacaagatgg
     8161 ttgtgttcaa cgagaacaaa accctgctct tcaagttgtc gattgtcggc acgtggccca
     8221 gcggaaccgc caacaggtca atgcagctaa ccttttccgg ctctgttcct gacacactgg
     8281 tcagcagtcg taatgcggcg acaacaaccg acaacatcct gttagctacg ttcttcagcg
     8341 tggataaaga cggctttctt gccacaaatg gcagtacgtt aaccattcag tcgaatggtg
     8401 cggcgtttac tgccacaacc atcaagataa tcgcggagca gtgatgatta cattcaaacc
     8461 aacgcgaaac atcgacctga tagaagcagt cggaaatcac cctgacatta tcgccgggag
     8521 caacaacggt gatggatacg actacaaacc tgaatgccgt tactttgagg tgaacgtgca
     8581 cggtcagttt ggcggcattg tttactatca ggagattcag ccgctgacat tagattgcca
     8641 cgccatgtac ctgccagaga ttcgcggctt cagcaaggaa attgggctgg cgttctggcg
     8701 atacattctg actaacacca ccgttcagtg cgtaacatcg ttcgctgcac gcaaattccg
     8761 acacggtcag atgtactgcg caatgattgg ccttaagcgt gtaggaacca tcaagaaata
     8821 cttcaaaggc gtggatgacg tgacttttta cagcgccaca cgcgaagaac taatcgactt
     8881 cctgaatcac gggagatagc catgttatat gcatttaagc tgggcagaaa actgcgcggc
     8941 gaggaacctt attgccctga aaaaggcggg aaaggtggca gttctgataa aagcgcaaag
     9001 tatgcagcag aagcccagaa gtatgctgca gacctgcaaa atcagcagtg gcagacgatc
     9061 atgaaaaacc ttgctccgtt cacgcctctt gcggagcagt atgttaacca gcttcagaac
     9121 ctttccagtt tagaaggtca ggggcaggca cttaatcagt attacaactc taagcagtat
     9181 aaagaccttg cagggcaggc gcgttaccag agtcttgctg ctgcggaggc gacgggtgga
     9241 cttggttcga cagccacaag caatcaactg gctacgatcg cgccgacact cggtcagtct
     9301 tggttatcaa atcagatgag caattacaac aatctggcaa acgttgggct tggtgcgctg
     9361 caaggtcagg caaacgccgg gcagacgtac gccaacaaca tgagcagcat tgcacagcaa
     9421 agcgcagctc ttgccgctgc taatgccaat aaaccatcaa gtcttcagac tgcaattagc
     9481 ggtggcacgt ctggtgcgat tgccggtgca ggtcttgcca gccttttggg aacatcaacg
     9541 ccttggggcg ctggtattgg tgctggtatc ggattgcttg gctcgttgtt ttaaggggga
     9601 atcatggcta cttggcaagg aacaaacggc ggattgttgg ctggtatcgg cggcgtcaac
     9661 tcaaacgctc cgagcgtaaa tgacatcggc aatacgcttc agcttatcag gcagaacaat
     9721 gatattgagc gttcaggcgc taacaatgtt gggctgactg ctttgcaagg tctttcaggt
     9781 attgcgggtg tttttcagca ggaaaagcag gctcagcggc agaaagaatt tcagcaggcg
     9841 tacgctaatg cttatgcgtc tggtgatcgc ggtgctttgc gtcagttggc tactcaatat
     9901 ccagaccaga ttgaatccgt tcgtaaaggc atgggattca ttgatgaaga gcagcgtaat
     9961 tctatcggca ccttagcggc tggcgcacgc cttgcgtcat cgtctccaga agcaatgcaa
    10021 tcatggctgc aaaacaacgc cggtgagtta gctcgtgttg gcgttaatcc tcatgacgtc
    10081 gctcagatgt accaacagaa cccgcggcag ttcggcgaat ttgtcgatca cctggggatg
    10141 aacagtctcg ggcccgaaaa atactttgac ctacaggata aaatgcaggg tcgccaggtt
    10201 accatgcgcg gtcaggatct ggattcgcaa accgccgctc ggaatcaggc aatcacaatg
    10261 cgcggacaag atatccaggc gaatttaggt cagcagcgca ttaatctgga cgcagaaaca
    10321 aaccgcatta acaacgaaaa taagcgcctt gaccggatgc tatcagcaga aactaacgac
    10381 ctgaagcgcc aggaaataca gagccgcata gcagccaaca accagcagtt gcagcagaag
    10441 cagcaagcgc taaatgatgg ctacaaagac ggcatcaaca ccctcacaac cagcatgttc
    10501 actctgaacg atatcgttag ttctccttca cttaagagca ttacaggctt acgtggagta
    10561 atccccaacg ttccaggctc acaggctgca gacactcagg cacgacttga tacctttaaa
    10621 tcacaagcat acctgacagc ggttcaggcc atgcgaggca tgggcgcact ttctgatgcc
    10681 gagggcaaaa agctcgacca ggctgttggt tcgctgcaga actcgcagag cgaggagtcc
    10741 tttcgtcgca acgctggcgt catcctgaac acgctcaacc agaagcgtaa tgaggcggtt
    10801 ggtaagtacg ttcagcaaaa cggtatcaag cgagtggaag cgcctcaggc ttctatagat
    10861 tacctgaagc agcaccccga gctgtcaatc gacttcatta atcgctacgg atatcttcca
    10921 tctttggggc agtaaatggc taattaccgt gatttgttag agcaggctgg cgcacgttac
    10981 ggtgtgccag aagggttgat gactgcactg ggtgccaagg agtcttctta caaccctgcc
    11041 gcagtaagct ccgccggggc tgtaggattg actcaggtca tgcctgggac atggcgtgat
    11101 atgggttata ccgatgagca aatgcaaaac cccgaatatc aggctgacgc tggcgcgcgc
    11161 tatctggcaa agatgtacca gcagtttggt aactggcgtg actctcttca ggcttatcac
    11221 gacggtcccg gcaacgttat gaaggcaaag cgtggtgaat atacgccagg acctgaaggc
    11281 cgcggttacg ttgatgatcg ctttgctcaa tgggctggtg acccggtgac agactcaaca
    11341 gtcgaacagc gcgccacctc tgcaaaggta catcctcagc aagaccctaa caacccgttt
    11401 gcacaactgg aagtacagtc atccgaacaa gtatcggcat caggtgtgca gtcagaccca
    11461 aataatccat ttgctcagat tgagcagcag gcagccagtc agcagccacc tcaacccgta
    11521 agttctgtcg caccgaaacc tgttcagcaa caaacagtta atcaggccaa taatgcacca
    11581 acacgggaag aaccatcatt gatgcaacaa gctggcgatt ggctcactgg tggtcaaagt
    11641 gcagggcaaa ttgcagaaca ggctggtcgt ggtctggtaa acataccatt tgacgtattg
    11701 cagggtggcg caagtctgat taatgcaatc agtcaggggc ttggggggcc aaaagtattg
    11761 gatgatgttt atcgtccagt agacagaccg acagacccct acgcgcaagc tggagagtca
    11821 ataggcggtt atcttgttcc tggagcaggt gtagctggaa acatggtcat tggttctctc
    11881 gctgacgcgg cgaatcaacg gggtgatttt gccgaaaatg ccgctattaa tgccggactt
    11941 agcattgcta cgcatggcct gataaatggc gttacccgtg gtgttcgtgg tgcatcaaat
    12001 ataattagtg gcaataaaac atctgcacag agagcgacca ctgcgccaac agaaacatca
    12061 ccattctccg gtgatgccgc tgcagcaaca aatcctgcgg ttcatgccgc agaggcaaga
    12121 gtagcacaag gtgtaccaat gacgcctgcg acgaagaacc cagaggaagt cgttcgcaca
    12181 gtagcagcac aaaaaaggcc aaatctcgct tcatcgcttg atgaactaga tatcaatcct
    12241 caggctgaag ttctggagtc tgctgaaagg cttaatgttg attcattact cccttcacac
    12301 ttttccggga acgagcaata caaggcagtt gagcaagcaa tcaagtcccg tgcgggttct
    12361 gctctacagg tgcaggaaaa tgaagcaatc aggcagctag cacagggcgc gggtgagata
    12421 attgatcgcg tttccggtgc aaaagatgct cttggtatga gcgacaagtt tattgatacg
    12481 gtcaatggaa gaatgtctgc gctgatgaaa cgaagcgacc agctttatcg caatgttgaa
    12541 aaggcgatgc ctgcaggtgc aaaaattgat gcgccatcaa caaggtcaat gctcaaacag
    12601 gtggcagaag atcttggcgg gatgaaaaac cttgacccta ttgaaaagag agttgaggta
    12661 gcctgagttt aacggacact ccttcctgaa atagaatggc atcagaagga gctaataatg
    12721 agcagaaaaa ccca
//
LOCUS       DAEBXN010000113        12648 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.113, whole genome shotgun sequence.
ACCESSION   DAEBXN010000113 DAEBXN010000000
VERSION     DAEBXN010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12648
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.113"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            758..841
                     /locus_tag="KJL99_003367"
     CDS             758..841
                     /locus_tag="KJL99_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HBD4437973.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(895..2247)
                     /gene="gorA"
                     /locus_tag="KJL99_003368"
     CDS             complement(895..2247)
                     /gene="gorA"
                     /locus_tag="KJL99_003368"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HBD4437974.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(2319..3161)
                     /gene="rlmJ"
                     /locus_tag="KJL99_003369"
     CDS             complement(2319..3161)
                     /gene="rlmJ"
                     /locus_tag="KJL99_003369"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HBD4437975.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSGFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLSWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            3364..5406
                     /gene="prlC"
                     /locus_tag="KJL99_003370"
     CDS             3364..5406
                     /gene="prlC"
                     /locus_tag="KJL99_003370"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HBD4437976.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDGCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            5414..6166
                     /gene="rsmJ"
                     /locus_tag="KJL99_003371"
     CDS             5414..6166
                     /gene="rsmJ"
                     /locus_tag="KJL99_003371"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HBD4437977.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(6215..7684)
                     /gene="dtpB"
                     /locus_tag="KJL99_003372"
                     /pseudo
     CDS             complement(6215..7684)
                     /gene="dtpB"
                     /locus_tag="KJL99_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
     gene            complement(8002..8436)
                     /gene="uspA"
                     /locus_tag="KJL99_003373"
     CDS             complement(8002..8436)
                     /gene="uspA"
                     /locus_tag="KJL99_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HBD4437978.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            8830..9165
                     /gene="uspB"
                     /locus_tag="KJL99_003374"
     CDS             8830..9165
                     /gene="uspB"
                     /locus_tag="KJL99_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HBD4437979.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(9236..10735)
                     /gene="pitA"
                     /locus_tag="KJL99_003375"
     CDS             complement(9236..10735)
                     /gene="pitA"
                     /locus_tag="KJL99_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HBD4437980.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWFSLQFL"
     gene            10967..12166
                     /locus_tag="KJL99_003376"
     CDS             10967..12166
                     /locus_tag="KJL99_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBD4437981.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQVGRRVLLIDNGKILGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVCDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLASIVK"
BASE COUNT         3112 a         3171 c         3359 g         3006 t
ORIGIN      
        1 taacagaaat gacatattgc gctcctgatt gttgcaggta gtgtctctct tcgaagcgga
       61 taagtcaaaa acatatatga cttaacgaat gtgtaagtgc agaggaaggt aataggtgtg
      121 aattttgagt tggctattca tttgaaaggc ggtttgaatc aggacgtaaa aaaaccgact
      181 ttgcgtcggt tttttactct tcagcctgag ttggtggctc tggctggagt gagaatgccc
      241 acatctaacg gttcaatact attagccagt ggctaacatt gcaagcgtta gcgatttctt
      301 cagctggaga agggacagtt tgctatacat ggtggtgaaa gaggatttat atactttcat
      361 cgggtcgctg ccgtaccaag ccttgtccga atccggacgt aaaaaaaccg acttcgcgtc
      421 ggttttttta ctcttcagcc cagagttggt ggctctggct ggagtgagaa tgccatcatc
      481 taacgcatca atgctaatac catactgaaa ctattgcaag gttatgctgg ttatttagcc
      541 taattacact gtatgattat ccagttagct ctgaggcatt ttcactctgg caatgcgcat
      601 aaacgctttc aaagtcctgg tcagaagtac gggtggtgcc gttaactgat gctctggccg
      661 gagtgagatt gcccacatct aacaatgagg catgcgccgt gacaggcagt ggatgagtaa
      721 gcggatgcat tctcactcca tcgcatggag aaaacgggtg attgataaag caatcatcgt
      781 tcttggggcg ttaattgcgc tgctggaact gatccgcttt ctgcttcagc ttctgaactg
      841 atagcggaaa cgtaattaag ggctaagagc acactactct tagcccttta acatttaacg
      901 catggtcacg aactcttctg ccgccgttgg gtgaatggcg acggtattgt cgaagtcttt
      961 tttggttgcg cccatcttca gcgccaccgc gaagccctgc aacatttcgt ccataccaaa
     1021 gccaatgccg tgaataccga caatcttctc ttctggccca acgcacacca gcttcatgcg
     1081 gcacggctgg cggtgagtgg tgacggcggt atacatcgcg gtgaaagagg atttatacac
     1141 tttcacctga tcgtcgccgt actgctcgcg cgcctgcggt tccgttaaac caacagtacc
     1201 aatcggcgga tggctgaaga ccacggtcgg aatgttgctg taatccagat gctcatccgg
     1261 cttgttatta aacaggcgtt cagagagacg gcgacccgct gcaactgcca ccggtgtcag
     1321 ttccactgca cccgtgttat cgcccaccgc gtaaatacct tcaatattgg tgttttgata
     1381 tttatcgacg acgatatagc ctttttcgtt ggttttaacg ccagcggctt cgaggttgat
     1441 gttgtcattg gcaggctcgc gaccaatcgc ccaaatcagg caatccaccg tttcactgcg
     1501 accatcttcc agctccagcg tcaggctacc atcggcattt ttcactaccg ctttcgggat
     1561 ggcgttggta tgcaactgtg ggccttcggc gttcatcact tcgaccagcg tttcagagat
     1621 catcgggtcg aagctgcgca gcggcgcatg tttacgcaca aacagatgcg ttttcgcgcc
     1681 gaggccgtta atcacgccag ccagctcaac ggcgatgtaa cccgcgccaa caaccgccac
     1741 gcgctctggc aaagcaggaa gggcgaagaa gccatcggaa tcaataccgt attccacgcc
     1801 tggaatatcc gggtggctcg gacgaccgcc ggtggcgatc agaatatgat cggccgtgat
     1861 ggtttcgccg ttaacttcca gcgttttggc atcaacgaag cgggcaaagc ctttgattac
     1921 atcaacgtta tttttaccga gcacgttttc ataggaagta tgaatacggt cgatataggc
     1981 ggtacggctg gcgatcaacg tttcccagtt gaacttgttg atggtggtat cgaaaccata
     2041 atcagggccg tacatatgga tcgcttcacg gatttgcgcc gcgtgccaca tcactttttt
     2101 cggcacgcag ccgacgttca cgcaggtgcc gcccagctct ttggcttcaa tcagtgcgca
     2161 tttctggccg tacatagccg cgcggttgat ggaggcgata ccgccgctgc cgccgccgat
     2221 ggcgatgtaa tcatagtgtt tagtcatgac atattgtcct tatcgttgat taccgcgatt
     2281 gtagcgcgag acgtaatagg tgccagcaat ggctgcaatt actccggcac gatccagctt
     2341 acggtggcgt gcccggtacc tgccggaacc agtttgctgt gcagccacga cagcacgtta
     2401 ttcatctgct gttccagctt ccacggcggg ttaatcacaa tcatgccgga agcggtcatg
     2461 ccacggcgat cgctgtctgg cagcaccgcc agttcaattt gcagaatttt gcgaatgccg
     2521 gtcgcttcca ggtcgtggat catgcgctta atttgctggc gcagcaccac cggataccac
     2581 aatgcgtaag tgccagtggc gaaacgtttg taaccttctg ctatcccgct gaccaccgcc
     2641 tgatagtcgg ttttcatttc atacggcggg tcgataagga ttaaaccacg gcgagaaacc
     2701 ggcggcagtt tggccttaag ttgctggaaa ccgtcggctt tttcgacgcg tgcacggctg
     2761 tctttctgaa atccagaacg cagcaacggg taatcgctcg ggtgcagttc ggtcagttgc
     2821 aggctgtctt gttcgcgcag tagctggcga gcaatcaacg gcgaaccggg gtagtaacgc
     2881 aactggccgc tacggttgaa gtgttttacc acattgatgt acgcctccag ttctgcgggc
     2941 aaatcgtcct gctgccagat acgggcgata ccttcgagat attcgccggt acgctcggca
     3001 tgttcgctac ctaactgata acgcccggcc cctgcgtggg tgtcgagata gagaaacggt
     3061 ttatctttct ctttcagcga ctcgatgatc aggctctgaa cggtatgttt aaggacgtcg
     3121 gcgtggttgc cagcgtgaaa gctgtggcga taactgagca tgggtaaagg tgttccggta
     3181 agtaaaaatt ggcccgtatt cggggcgcac aaaagcctat caggacagta taccgaaaag
     3241 aggccgccgc cgcgaaagcg taacgtttct cattgaaatt cactacactt aaccccatgc
     3301 tacacacatt atgtaaagcg cctgttgagc gcttccttaa cctctttaac caggactgcg
     3361 ctaatgacga atccgttact gactcccttt gaattgcctc cgttttctaa aattctcccg
     3421 gaacatgtcg ttccagccgt gactaaggca ttgaacgact gccgcgaaaa tgtggagcgc
     3481 gtagtagcgc aaggggcacc gtacacctgg gaaaatctct gccagccgtt ggcggaagtg
     3541 gacgatgttt tggggcgtat cttctccccg gtcagccacc tgaactcggt gaaaaatagc
     3601 ccggaactgc gtgaagcgta cgaacaaacc ctgccgctgc tgtcggaata cagcacctgg
     3661 gtagggcaac atgaagggct gtataaagcg taccgcgacc tgcgcgatgg cgatcattac
     3721 gccacgctga acacggcgca gaagaaagcg gttgataacg ccctgcgtga ttttgaactc
     3781 tctggcatag gtctgccgaa agagaaacag cagcgttacg gcgaaattgc tacccgtctt
     3841 tctgaactgg gcaaccagta cagcaacaac gtcctcgatg cgacgatggg ctggaccaaa
     3901 ctcgttaccg acgaagcggg gctggcgggg atgccagaaa gcgcgctggc tgcggcaaaa
     3961 gcccaggccg aagcgaaaga gctggaaggc tacctgctga cgctggatat cccaagctac
     4021 ctgccggtaa tgacctactg cgacaaccag gctctgcgcg aagagatgta tcgcgcttac
     4081 agcacccgcg cctccgatca aggcccgaat gccggtaagt gggataacag caaggtgatg
     4141 gaagagatcc tcgcgctgcg tcacgaactg gcgcaactgc tgggctttga aaactacgcc
     4201 tttaaatccc ttgccactaa aatggcagaa aacccgcagc aggtgctgga tttcttaacc
     4261 gatctggcaa aacgcgcgcg tccgcaaggc gaaaaagagc tggagcaact gcgtgccttc
     4321 gctaaagccg aatttggtgt cgatgagttg cagccgtggg atatcgctta ctacagcgaa
     4381 aaacagaaac agcacctcta cagcatcagc gacgagcagc tgcgtccgta cttcccggaa
     4441 aacaaagcgg ttaacggcct gtttgaagtg gtgaaacgta tttacggcat caccgctaaa
     4501 gagcgtaaag atgttgatgt ctggcatccg gatgtacgtt tcttcgaact gtatgacgaa
     4561 aataacgaac tgcgcggcag cttctacctc gatctgtatg cccgtgaaaa caagcgcggc
     4621 ggggcgtgga tggatggctg cgtaggccag atgcgtaaag ctgatggttc tctgcaaaaa
     4681 ccggtcgcgt atttgacttg taacttcaac cgcccggtaa atggtaaacc ggcgctgttc
     4741 actcacgacg aagtgatcac cctgttccac gagttcggtc acggcctgca ccatatgctg
     4801 acccgcatcg aaaccgctgg tgtttccggt atcagcggtg tgccgtggga tgcggtcgaa
     4861 ctgccgagtc agtttatgga aaactggtgc tgggagccgg aggcgctggc gtttatctct
     4921 ggtcactatg aaaccggcga accgctgccg aaagagttgc tggataaaat gctggcggcg
     4981 aagaactacc aggcggcgct gtttattctg cgtcagctgg agttcggcct gtttgatttc
     5041 cgccttcatg ccgagttccg cccggatcag ggggcaaaaa tcctcgaaac tctggcagaa
     5101 atcaagaaac tggttgccgt ggtgccatct ccgtcctggg gccgtttccc gcacgctttc
     5161 agccatattt tcgccggtgg ttatgcggca ggttactaca gctacctgtg ggctgacgta
     5221 ctggcggcag atgctttctc gcgctttgag gaagagggca ttttcaaccg tgaaaccggg
     5281 cagtcgttcc tcgacaacat tctgagccgt ggcggttcag aagagccgat ggatctgttc
     5341 aaacgcttcc gtggtcgtga accgcagctg gatgcgatgc tggagcatta cggcattaag
     5401 ggctgatcat tcagtgaaaa tctgcttaat tgatgaaaca ggcgccggag acggcgcctt
     5461 atctgttctg gcggcccgct gggggctgga gcacgatgaa gacaacctga tggcgctggt
     5521 gttaacgccg gaacatctgg aattgcgcaa gcgtgatgag ccaaaacttg gcggcatctt
     5581 tgttgatttt gttggcggag cgatggcgca ccgacgcaaa ttcggcggtg gtcgcggtga
     5641 ggcggtggcg aaagcggtgg gcattaaagg cgattatttg ccggatgtgg tggacgccac
     5701 cgcagggctg gggcgcgatg cctttgtact ggcttcagtc ggctgccgcg tgcggatgct
     5761 ggagcgtaat ccagtggttg ccgcgctact cgacgacggc ctggcgcgtg gttatgcgga
     5821 tgcggaaatc ggcggctggt tgcaggagcg gttgcagtta attcacgcct ccagcctgac
     5881 ggcgctaacc gatattaccc cacgcccgca ggtggtctat ctcgatccga tgttcccgca
     5941 taagcagaaa agcgcgctgg tgaaaaaaga gatgcgtgtc tttcagtcgc tggtggggcc
     6001 ggatcttgat gccgatggat tactggagcc tgcacgcttg ctggcgacca aacgcgtggt
     6061 ggtaaagcgc ccggactacg cgccaccgct ggcgaatgtc gccacgccaa acgcggttgt
     6121 gactaaaggg catcgctttg atatctatgc aggtacgcca gtgtaaaagt aaagcagggt
     6181 agcgataacg ctaccctgct ttctgcaaga ataattaatg gctttccggc gtcgcaatca
     6241 tgcgtttcag ccacggcacc atcaacagca tcactactgc aacgcccagc gtgaccagac
     6301 caatcttacc aaacacgttg gtatagacgg gcaacgtctc aagcggatcg gtaatgttgt
     6361 ccggcaccgc ggtaaatgtt gccacatagc cgcccagcaa gaacgcggca gcctgcgtca
     6421 ggaaccacat cccgagaata aagcccatca aatgctgcgg caccagggca gcaatcatcg
     6481 ccaggccaag ggcgctaata aacaattcac ccaggctcta gaataaatac accagcacga
     6541 taaaccatgg cgatgtcagc ccctggtcat ccgcaaacca cattcccgca gcagcagccg
     6601 tcaaaaagcc cagtgagcac ataaacatgc tgagagtaaa tttcatcggc atcgagaggt
     6661 ctttgccttt gttacccaga tgcgtgtaaa tgcctgccag tattgggctg gcgagtacca
     6721 cccagaacgg gttaagcgcc tggaagctga ccgggttgat ggaaaaaccg agaatttcat
     6781 gatgcacgtt gttgatggca aagaagttca gcgatgttgg catctgggcg tagagaatgt
     6841 aaaacaccac cgcttcgagc atcaggacaa aggcgacaaa cattttattg cgcccggttt
     6901 tatccagctt gaatgcctga cgaaagaaga tgatagtgac gacgatggag agaacaatca
     6961 gcaccagatt ggcgacttct acgttgtgca tcagccatgc acatacgaag atcatcacca
     7021 cgctgccaag taatacgtac aacagtttgc tgaagctcat cggcttgaag tcgggttcag
     7081 aaccaatgtc tttcaccatt ccacgacagg cgatgtaaac cagtaatgcg ataattaacc
     7141 ccgccccgca caggttgtag gtgactgaat aaccgaatct atcagcgatc acaggggcca
     7201 gcgataacgc tatcaacgag ccgatattga tcgacatata gaacagggtg aatgcgccat
     7261 caagccgcgg atctttcggc ggatagcact tcgaaagcaa gctggctggg ttagctttaa
     7321 acaggccgtt accgacagcg atagtcccca gggcgatgaa aatcaggtca ggcttaagta
     7381 gcgacatgcc ggtcatgaag tagccaatcg ccagcacaag tgctccaaga acaatggtgc
     7441 gtttggtccc caacaggtgg tcgccgacat agccgccaat ggaaatgagg ccatagacca
     7501 gtgcagcaaa agcaccaaaa gtgacaaaag cctgctcttg cgagaatcca agctgtttaa
     7561 cgaagaaaac cgccagtacg ccctgcacgc cgtagtagcc gaatcgctcc cataactcga
     7621 caaaaaagat catgaaaaat gggcgaggtt gctgcagcat ccccatgggt gttgttgtat
     7681 taatatttat taaccttcca ataccatcct gaaaagtatg aacgcgtaag ggataacgcg
     7741 cgaaaaagtt tacatctgtc agagattctt aatattgatg cggtgaatag attaatacac
     7801 tattacaaca gaaaataacc agctgatcac actaatgatg gaaaatatga tggtgttaaa
     7861 ttttgtattg gcaggagatt ttgattgcgt acgcagatcg taggccagat aaggggttta
     7921 cgctgcatca ggtaacaagg cgcgccaaaa agcaaaacgc ccgcatatgc gggcgttcag
     7981 gaacacgtcg tggagggatg attattcttc ttcgtcgcgt agcggaacaa tcagcatatc
     8041 aacgtgaacg gtgttgatca gctgacgtgc ggaagacatc agtttgctcc agaagtcctg
     8101 gtggtgacca caaaccacca gatccatatc gtatttcttg attgcatcga ccagaacctg
     8161 gcctaggtcg ccgctgccgc tcagggtttc agtgattgga tagcctgcat tagtggaaag
     8221 ctcggtcagc gcatgatgtg tctcttcaga gatgcgtttc tgcatatcac ccagattcac
     8281 atcaataagc ccggtgtata ggtcagagta gtttacatct acgtggatca gagaaacttt
     8341 cgcattgtag gggcgagcca tagagactgc tttctctacc agaactttgc tttccgggga
     8401 gagatcgact gcgatgagaa tgtgtttata agccatagtg ttactccttc cataaagttg
     8461 tcgatgactg gccagctagc gtttcttgtg ccgttacgtc acccgcgtcc tgcgaactac
     8521 gtgcgcgggg ctaacccagc cgtaaagatt cagtgttaag accatcctta ccttatagcg
     8581 acccggatga tccgtcaatc cgccttgctt acccgttaga taaacaaatt ttcgacatga
     8641 atattatatt gatagtggtt aaccttctgg aaaaaaaaca acctgatctc ctacactatc
     8701 tatagagccg ctcggatgtt ggtcaccaca aaagcggttt ctccaggacc tggtgttaac
     8761 tgattggcag cgtatcggga gcggtagccc cggggtggaa ctccgcgggc aggtcgccgg
     8821 ggaggaggta tgataagcac cgtcgcatta ttttgggctt tatgtgtcgt ttgcattgtt
     8881 aacatggcgc gctatttctc atcactacgc gcgttgttag tggtactgcg taactgcgat
     8941 ccattgctct atcaatatgt tgatggaggg ggctttttta cctcacatgg ccaacccaac
     9001 aaacaggtgc gtctcgtttg gtatatctat gcccaacgtt atcgcgatca tcacgatgat
     9061 gagtttattc gccgctgtga gcgggtgcgt cggcagttta ttctgactag cgcattgtgt
     9121 ggtctggtgg tggtcagcct gattgcattg atgatttggc attaagcact tcagcaataa
     9181 taacgtataa aaaaagcggg ccagtaaact gacccgctcg ttttggtgcg tacgattaca
     9241 ggaactgcaa ggagaaccag tacagcccgc cggaaagtag taccgcagcc ggaagggtaa
     9301 acacccaggc catcagaata ctggttacgg ttttacgctg taagccgccg ccatctacca
     9361 ccatcgtccc cgcgacagaa gaggagagta cgtgagtggt ggaaaccggc atcccggtat
     9421 aactcgccag gccgattgac actgccgccg tcatctgggc agacatcccc tgagcgtagg
     9481 tcatgccttt cttaccgatt ttctcaccga tagtcgttgc cacacgacgc cagccaatca
     9541 tcgtaccgat acctaacgcc agcgcgaccg ccatgatgat ccacaccggc gcatactcga
     9601 tggtgctaag catgtcggac ttcagtttct tcaacaggcg ctgatcgtca gcactcacac
     9661 caggcatctt caccactttg tcgatagtgt cagaaacgca cagcataatg cggcgcatct
     9721 ggctacgttg atcaagcgac agcttgtcgt agctttccac atcggtggtc aacatacctt
     9781 tcaggcggtt gagcgcgtta atggtattcg acggatggca gtggaactcc gcaggttgcg
     9841 ttgcgccagc ttccggagcc ggtactaact gatcagcacc ggtagcctgt ttgagcagcg
     9901 caggatgctg ctcaaagtaa gcttcgacgt tgttgatggc atcacgggta cgggtgattt
     9961 cgtagccagt ggcattcatg ttcaccacga agcctgctgg cgcgacgcca atcaatacca
    10021 acataaccag accaatgcct ttctgaccat cgttcgcgcc gtgcgaaaac gccacgccga
    10081 tagcggaaag gatcagcgcg atacgcgtcc agaacggcgg ctttttcttg ccgtctttct
    10141 tttcacgctc cgctggggtc aggtggatac gggcgcgttt cttggtgccg ctccagtagc
    10201 gacgcagcaa gaaaatcaga ccgccagcaa acaccaggcc gacaataggg gaaacgatca
    10261 gagaaccgaa aatacttaat actttcggga tattgagtgc atccaccact gacgtcccgg
    10321 tcatcaacgc attggttaaa ccaatcccga tgatcgcgcc aatcagcgta tgagagctgg
    10381 atgcaggtaa accaaagtac caggtaccca ggttccagat aatcgccgcc agcaacatag
    10441 agaacaccat ggcaaggcca tgagacgatc ccatattaag cagcagatcc gtcggcagca
    10501 tatgcacaat ggcataggca acactcagac cacccagcaa aacacccaaa aagttgaaca
    10561 ccgccgccat aaccacggcg agctgagaac gcatcgcgcg ggtatagata acggttgcca
    10621 cggcgttggc tgtgtcatgg aaaccattga tggcttcgta gaacagcaca aaagccagtg
    10681 caagcaataa taacagcccg gtatgcaaat ccaggccagc aaacaaatgt agcataggac
    10741 gttacgccat tttgaggaca tgaacgcggc gcattatcag tgactttcgc ggcgcgggca
    10801 aagtgaaata tagacttttt ttgatttgcc tcctgtatgg atttcactca aaaaataatt
    10861 atcttatata attcaggcaa atacttcctt ttagtaatat tgatgctggt gcgaccactg
    10921 aggaatcttt acaattcacg cccgtttttt ctaagaggag cgcaacgtgg aaaggtttga
    10981 tgccattatt ataggcgctg gtgcggcggg tatgttctgt tctgcgctgg caggtcaggt
    11041 aggacgccgg gttctgctga tcgataatgg taaaatacta gggcgcaaaa tccttatgtc
    11101 tggcggtggg cgctgcaact ttaccaacct ttatgtcgaa ccaggcgctt atctgagcca
    11161 gaatccgcat ttttgtaagt ctgcactcgc acgttttacc cagtgggatt tcattgatct
    11221 ggtcaataaa cacggtatcg cctggcacga gaaaacgtta gggcaactct tctgcgatga
    11281 ctccgcgcag cagattgtcg acatgctggt ggatgagtgc gagaagggca atgtaacctt
    11341 cagattgcgt agcgaagtgc tgagtgtggc gaaggatgaa acaggcttca cgcttgatct
    11401 gaacggcatg actgtcggtt gcgaaaagct ggtcatcgcg accggtgggc tgtcaatgcc
    11461 ggggctgggc gcatcgccgt ttggttataa gattgccgaa caatttggcc tcaacgtgct
    11521 gccgacccgc gcgggtctgg tgccattcac tctgcataaa ccgttgctcg aagagttaca
    11581 ggtgctggcg ggcgtggcgg tgccttccgt gattaccgct gaaaacggca ccgttttccg
    11641 tgagaactta ctcttcaccc accgcggctt atctggaccg gcggtgttgc agatttcaag
    11701 ctactggcaa ccgggtgaat ttgtcagtat caatctgcta ccggatgtgg acctcgaaac
    11761 cttcctgaat gagcagcgta acgcacatcc gaatcaaagc ctgaaaaaca cactggcggt
    11821 tcatctaccg aagcggttgg ttgaacgctt acagcaactc gggcaaatcc cggatgtttc
    11881 gctaaaacag ctcaacgtgt gtgaccaaca ggcactgatt agcacattga ccgactggcg
    11941 cgtacaaccc aacggcactg aaggctatcg cactgccgaa gtgacgctcg gcggcgtgga
    12001 caccaacgaa ctttcttcac ggacgatgga agcgcgcaaa gtgcctgggc tgtacttcat
    12061 cggcgaagtg atggacgtta ccggctggct ggggggctat aacttccagt gggcgtggtc
    12121 gagtgcatgg gcttgtgcgc aggatttggc gtcaattgtg aaataaccat gacgttttcc
    12181 cgtcagccgt ttgtatatgt cgttattaag tcattttggt acggttctgc atccgacatt
    12241 ctggtgttaa ctcgctgttt tgttagcgaa ttaacgctat tgccgttgtg gttattttat
    12301 agtgtattta ttctcagaga atatatatta ctgaaataaa aaacacaaag aataaccaaa
    12361 ggaaattatt tgaaacgtaa aaggtatata tttttaatct gtaaaattta aaatcacctc
    12421 ctctggattg aatcaacttg ctctatttaa gacgattgtt tttacatttt caccacctgt
    12481 atcaggtggt gtttttttat tggcggcaat gcctttatct tatttttatt acgttgtaaa
    12541 tatggctatt cctaaatgaa atttgatttg ctcagtttat ttgtgatgtc aggggagtgc
    12601 ttgccatgag agtcgtgata ttattattca tcatttttag gtaatgct
//
LOCUS       DAEBXN010000114        12389 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.114, whole genome shotgun sequence.
ACCESSION   DAEBXN010000114 DAEBXN010000000
VERSION     DAEBXN010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12389
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.114"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            327..1967
                     /gene="trg"
                     /locus_tag="KJL99_003377"
     CDS             327..1967
                     /gene="trg"
                     /locus_tag="KJL99_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HBD4437982.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEAFDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVNVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            complement(2005..2928)
                     /locus_tag="KJL99_003378"
     CDS             complement(2005..2928)
                     /locus_tag="KJL99_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBD4437983.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSN
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSTSLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            3145..4488
                     /locus_tag="KJL99_003379"
     CDS             3145..4488
                     /locus_tag="KJL99_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBD4437984.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQLATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            4713..6368
                     /gene="opgD"
                     /locus_tag="KJL99_003380"
                     /gene_synonym="mdoD"
     CDS             4713..6368
                     /gene="opgD"
                     /locus_tag="KJL99_003380"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HBD4437985.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGISP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            6508..6732
                     /locus_tag="KJL99_003381"
     CDS             6508..6732
                     /locus_tag="KJL99_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HBD4437986.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            6795..7331
                     /gene="rimL"
                     /locus_tag="KJL99_003382"
     CDS             6795..7331
                     /gene="rimL"
                     /locus_tag="KJL99_003382"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HBD4437987.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQTLIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(7326..8306)
                     /locus_tag="KJL99_003383"
     CDS             complement(7326..8306)
                     /locus_tag="KJL99_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4437988.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYVQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            8430..9422
                     /gene="tehA"
                     /locus_tag="KJL99_003384"
     CDS             8430..9422
                     /gene="tehA"
                     /locus_tag="KJL99_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HBD4437989.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCMFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            9419..10012
                     /gene="tehB"
                     /locus_tag="KJL99_003385"
     CDS             9419..10012
                     /gene="tehB"
                     /locus_tag="KJL99_003385"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HBD4437990.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFTFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            10315..10971
                     /locus_tag="KJL99_003386"
     CDS             10315..10971
                     /locus_tag="KJL99_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HBD4437991.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYNNYSDLKETTSAT
                     GKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMKEAIA
                     QRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTMDTRL
                     YFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFDVNKK
                     "
     gene            complement(10989..11495)
                     /gene="tnpA"
                     /locus_tag="KJL99_003387"
     CDS             complement(10989..11495)
                     /gene="tnpA"
                     /locus_tag="KJL99_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HBD4437992.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTRYRRQIFDHDATEK
                     LRTYFSNVCAYFEAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGVSGRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCGGAPISVIRQYIEQQQTPGQVENRALYPRPEGWGFT
                     AHRIKSAI"
     gene            11503..12389
                     /locus_tag="KJL99_003388"
                     /pseudo
     CDS             11503..12389
                     /locus_tag="KJL99_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3228 a         2903 c         3113 g         3145 t
ORIGIN      
        1 tgatagaaat atcggtcggg gccttcgtct ttctgattcc cggttagcct gaaaacagaa
       61 agtctcaggc acccgcaggc atcctatgag gtttccttag ggacgaaaat aatcacttca
      121 cgaaattgcg tgctgttttc cagaattttt cgtcattcgg gttagccagt ttagccattc
      181 gttactctct tcattccaat agcattaatt ttctctgcaa taattgttgt aaaaatgtga
      241 cgcaaagagg tttttggtca taagtaatta ccgtcaagtg ccgatgactt tctatcagga
      301 gtaaacctgg acgagagaca acggtaatga atacaactcc ctcacagcga ttaggttttt
      361 tgcatcacat caggctggtt ccgttatttg cctgcattct tggcggtatc ttagttctat
      421 tcgcattaag ttctgctctg gctggctatt tcctctggca ggccgatcgc gatcagcgtg
      481 atgttactgc ggagattgag attcggaccg ggttagcgaa cagttcagat tttttgcgtt
      541 cagcccggat caatatgatt caggccgggg ctgcgagtcg tattgcggaa atggaagcaa
      601 tgaagcgaaa tattgcgcaa gccgaatcgg agattaaaca gtcgcagcaa ggttatcgtg
      661 cttatcagaa tcgaccggtg aaaacacctg ctgatgaagc cttcgacact gaattaaatc
      721 aacgctttca ggcttatatc acgggtatgc aacctatgtt gaaatatgcc aaaaatggca
      781 tgtttgaagc gattatcaat catgaaagtg agcagatccg accgctggat aatgcttata
      841 ccgatatttt gaacaaagcc gttaagatac gtagcaccag agccaaccaa ctggcggaac
      901 tggcccatca gcgcacccgc ctgggtggga tgttcatgat tggcgcgttt gtgcttgccc
      961 tggtcatgac gctgataaca tttatggtgc tacgtcggat cgtcattcgt ccactgcaac
     1021 atgccgcaca acggattgaa aaaatcgcca gtggcgatct gacgatgaat gatgaaccgg
     1081 cgggtcgtaa tgaaatcggt cgcttaagtc gtcatttaca gcaaatgcag cattcactgg
     1141 ggatgacagt agggactgtt cgacagggcg cggaagagat ttatcgtggc accagcgaaa
     1201 tttcagctgg caatgcggac ctgtcatctc gcaccgaaga acaagcggcg gctatcgaac
     1261 aaactgccgc cagcatggag caactcactg cgacggtgaa acagaatgcg gataacgcgc
     1321 atcatgccag caaactggcg caagaggctt ctattaaagc cagcgatggc gggcagacgg
     1381 tttccggtgt agtaaaaacg atgggcgcta tctccacgag ttcgaagaaa atttctgaga
     1441 tcaccgccgt catcaacagt attgctttcc agacgaatat tctggcactg aatgctgccg
     1501 ttgaagccgc gcgagcgggt gagcaagggc gtggatttgc cgttgtcgcc agcgaagtac
     1561 ggacactcgc aagccgcagc gctcaggcag cgaaagagat tgaaggcttg atcagtgaat
     1621 cagtcaggtt aattgacctg gggtcggatg aggtggcaac ggcagggaaa accatgagca
     1681 ctattgttga tgccgtcgcg agtgtcacac atatcatgca ggaaatcgcc gccgcctcgg
     1741 atgaacaaag tagaggcata actcaggtta gccaggcgat ttctgaaatg gataaggtga
     1801 cgcaacagaa tgcttctctg gtagaagagg cctcagcagc ggcggtgtcc cttgaagaac
     1861 aggctgcacg attaactgag gcggtgaacg tattccgtct gaacaaacat tctgtgtcgg
     1921 cagaacctcg cggagcgggt gaaccagtta gtttcgctac ggtgtgaaaa tgttcaagga
     1981 gggatcgaca gatcccttca cctttcagaa cggcattgat tttcgaatag cgttaatcat
     2041 caactggcaa ccagaagaga acgtcgcatc tacgcgggtc agtattccaa tcggttcgcc
     2101 tgcaccatgt cccggaacag gcagggccac cagcgtggca tgacgcaggt cgtcttttac
     2161 agcgccagaa gggacaaacc acacgtaatc gtattcaacg gtaagttgac gagatagcga
     2221 agtagacagc gtttcgatac aacccgaagg aattttacag ccctggctct gcactaatgc
     2281 atctgaatgc tggcgtggcg cagtgccttc tggtgataca acgaccggcc attccagcac
     2341 ccggcttagc gttacgttct cctgaagtag cgggtgatta gggcggacaa ccagcttcaa
     2401 cgattcaaga aacagcagtt cgtaattaag cccggtcatc agttcaggat ctgacatccg
     2461 accaatgccg atatcgattt ccccggtttt taaacccgcc agaatcatag ggttactcat
     2521 tgtcgcaact tgcaaggtcg tctctttttg ttgctgatga aactgaccta taaccgaagg
     2581 taatatcccc agtgccgcag taggtagtgc accaaccctg acgacatcat tattaagacc
     2641 ttctttacga tgaagcgact gtccggcagt gttgatggcg tcaagaactc tgactgcatg
     2701 cgttaaaaat tgttcgccgg gtaaggtaag ttgcgccccc tgacgaccac gctcaaacaa
     2761 gcgagcgccc gtcagctgct ccagttcatt caatgtatta gagagcgcag gttgactcaa
     2821 attaagggtt tcagctgcgc gccccaaagt tccttgttgt gcgacagcta cgaatgtatg
     2881 aaggtggcgc aaacggatgc gctgactaaa cagactattt ttttccataa gcgatgttaa
     2941 aaacgaagcg gtgtcgctga caagtgaagt tgtttgatta tgataacttg attgcaaaat
     3001 attattaaca attaaagcaa ttatgttaca gcaaaatgga taatattgat gttttcgcgg
     3061 cgagatcaca gtttgtaaat tcttcccgcc agagtgaatg cggttaccta cactccagat
     3121 tactgaccac tggaggcaga cactatggcg aacagcatca cggcggatga gattcgggaa
     3181 cagttttcgc aggcaatgtc agccatgtac cagcaagaag ttccgcaata tggcacgctg
     3241 ctggaactgg tagctgatgt gaatctggct gtgctggaaa acaatcctca actgcacgaa
     3301 aaaatggtaa atgcagacga gctggcgcga ctgaatgttg aacgtcatgg ggcgattcgc
     3361 gttgggactg cacaagagct tgctactctt cggcggatgt ttgccattat ggggatgtac
     3421 ccggtgagct attacgatct ctcgcaggca ggggtgccgg tacattcgac agcatttcgg
     3481 cccattgatg atgcttctct ggcgcgtaat cccttccgcg tttttacatc cttactccgc
     3541 cttgagctta tcgagaacga aattttgcgc cagaaagcgg cggagattct gcgtcagcgc
     3601 gatatcttca ccccacgttg tcgacaactg ttagaggaat atgagcagca gggcggtttt
     3661 aacgaaacac aggcacagga gtttgtgcag gaagccctgg aaacgtttcg ctggcaccag
     3721 ttagcaacgg tagatgaaga aacctatcgc gccttgcaca acgaacatcg gttgattgct
     3781 gatgtggtct gttttcctgg atgccatatc aaccacctga cgccacgtac gctggatatt
     3841 gaccgggtgc agtcgatgat gcctgaatgc ggaattgaac ccaaaattct catcgagggg
     3901 ccgccgcgcc gcgaggtacc gattttacta cgccagacca gctttaaagc actggaagag
     3961 acggtgttgt ttgcggggca gaaacagggc acgcataccg cgcgctttgg tgaaattgag
     4021 cagcgtggcg tggcattaac gccgaaaggg cgacaactgt atgatgatct tctgcgtaac
     4081 gctggaaccg ggcaggataa tctcactcac caaatgcatt tacaggaaac cttccgcact
     4141 tttcctgaca gtgagttttt aatgcgtcag caagggttgg catggttccg gtaccgtctg
     4201 acgccttcgg gtgaggcgca tcgtcaggcg attcatcctg gagacgatcc acagccctta
     4261 attgaacgtg gttgggtagt ggcgcaaccc atcacctatg aagatttctt gcccgttagc
     4321 gcggcgggga tcttccagtc aaatctgggt aatgaaacgc agacacgcag tcacggtaat
     4381 gccagtcgcg aagcatttga gcaggcgttg ggttgtccgg ttttggatga gttccagctt
     4441 taccaggaag cggaagaacg cagtaaacgt cgctgtggtt tgctttaaat tctgaccatc
     4501 cgcctttgca aaaatttgcc tgattttaca aacgaatcag gctcatccca tcgacataaa
     4561 aaaaatgccg atttatgcat attctctcag ttcaacaatt ggattattaa taaatattgt
     4621 ctagagtgag cggtcataaa taagcacttt cttgccgctg aaaacgacca gcgcgggacc
     4681 attcacaaca ccagaaggac tcactttcag gtatggatcg tagacgattt attaaaggtt
     4741 caatggctat ggccgccgtg tgcggtacca gcggcattgc ttctcttttt tctcaggcag
     4801 cattcgcggc agattctgat attgccgacg ggcaaaccca gcgttttgac ttctccattc
     4861 tacagtcaat ggcgcacgac ttagcgcaaa cagcgtggcg tggtgcgcct cgtccgttac
     4921 ctgacacgct ggcgacaatg acgccgcagg cttataacag tattcaatac gacgccgaaa
     4981 aatcgctctg gcataacgtt gagaaccgtc aactggacgc tcagttcttc catatgggaa
     5041 tgggattccg tcgccgcgtt cgtatgtttt ctgtagatcc agcaacacat ctggcgcgtg
     5101 aaattcactt tcgcccggag ttgttcaaat acaacgatgc aggtgttgat accaaacaat
     5161 tagaagggca aagcgatctc ggctttgccg gttttcgcgt gtttaaagcc cccgaactgg
     5221 cgcgccgtga tgtagtatca tttctcggcg cgagttattt ccgcgccgtt gatgatacat
     5281 atcaatacgg tttgtcggcc cgcggcctgg cgatcgacac ttacaccgac agtaaagaag
     5341 agttccccga ctttaccgcc ttctggtttg atacggtaaa accgggggca actaccttta
     5401 ccgtttatgc gttgctcgat agcgccagca ttactggtgc ctataagttc actatccatt
     5461 gtgagaaaag tcaggtgatt atggatgtgg aaaatcacct gtatgcgcgc aaagacatta
     5521 aacagctggg catttcgccg atgaccagta tgttcagctg cggtactaat gaacgtcgga
     5581 tgtgcgatac aattcatccg caaattcatg actctgatcg tctgtccatg tggcggggca
     5641 acggcgagtg gatttgccgt ccgctgaata atccgcaaaa attgcagttc aatgcttaca
     5701 ccgacaacaa cccgaaaggg tttggtttat tgcaactgga tcgtgacttc tcccattatc
     5761 aggacattat gggctggtat aacaaacgcc caagtctgtg ggtggaaccg cgtaacaagt
     5821 ggggtaaggg caccatcggc ctgatggaaa tcccaacaac gggcgaaacg ctggataaca
     5881 ttgtctgctt ctggcagcca gaaaaagctg taaaagcagg tgatgagttt gcattccagt
     5941 atcgtctgta ctggagtgcg caaccgcctg ttcattgccc attagcgcgc gttatggcga
     6001 cgcgtaccgg catgggcggt ttcccggaag gttgggcgcc aggtgaacac tatcccgaaa
     6061 aatgggcgcg tcgttttgcc gtcgatttcg ttggtggtga tctgaaagct gccgcgccaa
     6121 aaggcattga gccggtgatt acgctttcca gtggggaagc gaagcaaatc gaaattctct
     6181 atattgaacc catcgatggt tatcgtattc agtttgactg gtatccgact tcggactcca
     6241 ctgatccggt cgatatgcgg atgtatctac gttgtcaggg ggacgctatc agtgaaacat
     6301 ggctgtatca gtatttcccg ccagcgccgg ataaacgtca gtatgttgac gaccgcgtga
     6361 tgagttaatc gttttttctt cggcaccttc ttcgggaggt gccgtctggt taaacacgat
     6421 cccgctcgca tttttcccta agttaaatga gtaatctgat ggtgtgtatt tcagatacac
     6481 cgtgtcagcc actaacaggg agtgcgtatg tttccagaat accgagattt aatatcccgt
     6541 ctgaaaaacg aaaatcctcg ctttatgtcc ttgttcgata aacacaataa acttgatcat
     6601 gaaattgcca gaaaggaagg ttccgacggt cgagggtaca atgcggaagt ggtccgcatg
     6661 aaaaaacaaa agctacagtt aaaagatgag atgctcaaaa tcctgcagca ggagagcgtc
     6721 aaagaggtgt aaactttcct aagccgccag ccaggcggct tttttaacaa cagcatggat
     6781 tgactggaga taagatgact gaaacgataa aagtaagcga atcacttgaa ttacatgctg
     6841 ttgcggaaag tcacgtcaca cctctttatc agttaatctg taaaaataaa acctggttac
     6901 agcagtcgct aaactggccg cagttcgttc agagtgaaga ggacacgcga aaaacggtgc
     6961 agggtaatgt gatgttgcat caacgcggct atgccaaaat gttcatgatt ttcaaagaag
     7021 atgaacttat cggcgttatc tcgtttaatc gtattgaacc actgaataaa actgctgaaa
     7081 tcggctactg gctggacgaa tctcatcagg ggcaggggat catttctcag gcgctgcaga
     7141 cattgattca tcattacgct cagtctggtg aacttagacg cttcgtgatc aaatgtcggg
     7201 tggacaatcc gcaaagcaac caggtcgctt tgcgcaatgg ttttatcctt gaaggttgcc
     7261 tgaaacaggc tgagttcctg aatgatgcct atgatgatgt gaacttatac gcgcgtatta
     7321 tcgattcata ataaccctgc cagcggcgtt cgcgtaatgc gctcctcgcc gttaatgact
     7381 tttggaccac ggacatgaac cgtgtcgcca tcaaaggctt caacgacggc gtggtcggtt
     7441 aactccacat ggttttcaat tatcaccgca cctgtaatac ggctctcacc ttgtattaca
     7501 acgtgctcgt cgagcagaat cggtccacca cgtaccacgg catttccgcc aatcaagaca
     7561 tgatgtttta acacacaatt accttccaca atggcatatt ccgccacctg cgaactgtaa
     7621 tgaatcgtcg gaatggcatc ttcttctatg ccagctttca cctgcgcgtg accgtagact
     7681 ttagcgcaat cgcatagcca gacgttgttc tcttcattac cttcaacact ggcaaaatca
     7741 aaaacttcgg cgcgatgttc aataaaagca taccggacga cggcatcgcc ataaatttgt
     7801 gcctggtgga caatacgcga ggcgctaacc cttgcgcgat cataaatttg gagcaggagt
     7861 tgatggtcgg gcgttaagcc ttgtgcggcg acgatcatag aatgttgatc aattaaggcg
     7921 tgaccaaata ttctacattg cacgtaaact aatgagtcat gaatggtgac actgtcactt
     7981 atatatgcac cctggctgat ttcactatta tcgatccaga cgttatccgt tgcgtaaacc
     8041 tctccccaca gcacgctggt gccggtgata cgcgtattac cggaaatcac cgttccgccg
     8101 aacgcaatag cattctggtc gtaaatccag caatttcctt cctgtgctaa caccgtttcg
     8161 cgatcgatcc agccgccagc ggttcctgct atcacatcgt taaaatcgct aatggcgata
     8221 atctgccgta ataacacact ttttttagtg ccatcttcct gataactaaa ggctcgctgt
     8281 tcttcactta ggcgatattt gcgcatagcg ttttcccaca ggtgacttac tataaccgta
     8341 gcaaattctg cggctctggc tatgctcgag aaaattccat aaaatgcatt tcaaatatac
     8401 tttataaatt aaacaaaatg agtaagaaga tgcagagcga taaagtgctc aatttgccgg
     8461 caggctactt tggtattgtg ttggggacga tagggatggg atttgcctgg cgctatgcca
     8521 gccaggtttg gcaggtcagc cactggttag gggatgggct ggtgattctg gcgatgatca
     8581 tctggggatt attgactagc gcatttatta cccgactcat acgctttccg catagcgtgc
     8641 tggcggaagt tcgccatcca gtgctgagca gttttgtgag tttgtttccg gcaacgacga
     8701 tgctggtggc gattggtttt gttccgtggt ttcgcccact ggcggtgtgc atgttcagtt
     8761 ttggtgtcgt ggttcagttg gcttatgccg cctggcaaac tgcgggatta tggcgcggat
     8821 ctcaccctga agaagctacc acgcctggac tgtatctgcc gacagttgcc aacaacttta
     8881 tcagcgcaat ggcctgtggt gcgttgggct acaccgacgc cggtctggtg tttttaggcg
     8941 caggcgtttt ctcatggcta agccttgaac cggtgatctt gcagcgtctg cgcagttcgg
     9001 gagaattacc cacggcactg cggacatcac tcggcattca gctcgctcct gcgctggtgg
     9061 cctgtagtgc ctggctgagc gtcaacggcg gcgagggtga cacgctggcg aaaatgcttt
     9121 tcggttatgg actgctgcaa ctgctgttta tgctacgtct gatgccatgg tatctctccc
     9181 agccatttaa tgcttcattc tggagtttct cgttcggcgt atctgcactg gcaaccaccg
     9241 gtttgcatct ggggagtggc agcgataatg gatttttcca tacgctggcg gtgccgctgt
     9301 ttatctttac caattttatt attgcaatac tgctcatccg tacttttgcg cttctgatgc
     9361 agggaaaatt gttagtcaga accgagcgcg ccgttttaat gaaagcagag gacaaagaat
     9421 gatcattcgt gacgaaaact attttactga taaatatgaa ttaacccgca cacactctga
     9481 agtactggaa gcggtgaaag tggttaaacc gggtaaaacg ctggatctgg gctgtggcaa
     9541 tggtcgtaac agtctttacc tggcagccaa tggttatgat gttgacgcat gggataaaaa
     9601 tgccatgagt atcgccaacg tcgagcgcat taaatccatt gaaaatctgg ataatttaca
     9661 cacccgggtc gttgatctga ataacctcac atttgatgga cagtacgatt ttattctttc
     9721 gactgtggtg ctgatgttcc ttgaggctaa aaccatccca gggttgattg ccaatatgca
     9781 acgttgcact aaacctggtg gttacaacct gattgtggcg gcgatggata ccgctgatta
     9841 tccatgtacc gtcggcttca cgtttgcctt caaagaggga gaattacgtc gatattacga
     9901 aggctgggag agggtgaaat acaatgaaga cgtcggcgag ctgcaccgca ccgacgccaa
     9961 cggtaatcgt attaaactgc gtttcgccac gatgctggca cgtaaaaaat gacccggtaa
    10021 gcacaaaacg cgtgaaaatt ccccacgctg agatgattta ctgttcttct tttcggtaag
    10081 catatttttt atcgaaggga tgtgaaatta atcacagtag tcgaagtttt ttagcagctt
    10141 aacttactga aatttaacta ctgatgattg acttagcccc tttttcggca ttgactatgt
    10201 cgtctgaaaa ggggctgaaa aatttatttt caccaacact ttttttgcca caacacgaag
    10261 cggcgctttt tgctataact tagaaagtaa tataatcatc tcaggaaact attcatgcgt
    10321 accacatcat ttgcgaaagt tgcagcttta tgcggcttgt tggctctgtc tggttgtgcc
    10381 tctaaaatca cccagccaga taaatataac aattactctg atttaaaaga aacaacctca
    10441 gcaacaggta aacctgtttt acgttgggta gacccgagtt ttgatcaaag caaatatgac
    10501 agcatcgtct ggaacccaat cacttattat ccggtaccaa aaccttctac ccaggtaggg
    10561 cagaaagttc tggataaaat tttgaactat accaacaccg aaatgaaaga agcgatagcg
    10621 cagcgtaaac cagtggttac caccgctggg ccgcgtagtc tgattttccg tggggccatt
    10681 accggtgtag ataccagcaa agaagggctg caattctatg aagtggttcc tgttgcatta
    10741 gtggttgcgg ggacgcaaat ggctacaggc caccgtacca tggatactcg cctctatttt
    10801 gaaggtgagc tgattgatgc agcgactaat aaaccggtta tcaaagtcgt tcgtcagggc
    10861 gaaggtaaag acctgaataa cgaaagtacg ccaatggctt tcgaaaatat taaacaagtt
    10921 attgatgaca tggcgaccga tgccaccatg tttgacgtta acaaaaagta gttccagacg
    10981 cgccatcgtt agatggcgct ttttatccgg tgcgccgtaa aaccccatcc ttcagggcgg
    11041 ggatataagg cgcggttttc cacctgacca ggtgtttgct gttgttcaat gtattggcgg
    11101 atgacggata ttggcgcacc tccgcagcta ctggcaaaat agccaggact ccacaaaacg
    11161 cctttgtagt aatacctgac tgcaatatct ggtcgatctc gtcgcagtaa cctacccgat
    11221 acgcctttga ggctgtttac cagactggat atcgccagtt tggggggata gttaattaac
    11281 agatggacgt gatctggttc gccatccatt tcaaccagtt cagcttcaaa ataagcacat
    11341 acatttgaaa agtaagtgcg tagtttttct gtcgcgtcat ggtcaaaaat ctggcgtcgg
    11401 tatctggtga caaagactag gtgaacatgc atcaggaaaa cacaatgcct tccacgccgg
    11461 atatcggttt cttttttcac agaccaaagt atcatttgac ctgtgaaacg attacaggca
    11521 tttaaattcc agttaagacc aggtggtcaa caggagtgtg aaatgaggcg cttcgcaggc
    11581 gcatgtcgtt tcgttttcaa tcgtgctctg gcacgtcaga atgagaatca tgaggccggt
    11641 aataaataca tcccttacgg gaaaatggct tcctggctgg ttgagtggaa aaatgccact
    11701 gaaacgcaat ggcttaaaga ttctccctca cagccattgc aacagtcact gaaagacctt
    11761 gagcgggctt acaaaaactt cttccggaag cgggctgctt ttccccgatt caaaaagcgg
    11821 tgacagaatg atgcattccg ctacccgcag ggcgttaagc tcgatcagga aaacagccgt
    11881 atttttctgc cgaaactggg ctggatgcgc taccggaata gccgtcaggt cacgggtgtt
    11941 gtgaaaaatg tcactgtcag ccagtcctgc ggtacgtggt acatcagtat tcagacagaa
    12001 agtgaagtat ccactccggc tcacccttca gcatcaatgg tcgggctgga tgctggcgtg
    12061 gctaaactcg ccacgctgtc agatggcaca gtctttgagc ctgtaaacag ttttcagaaa
    12121 aaccagaaga cgctggcgag acttcagcga cagttaagcc gcaaggtcaa attcagcaac
    12181 aactggcaga agcagaaacg caaaatacag cgactgcatt cctgtatcgc aaatatccgc
    12241 agggactacc ttcacaaagt cacaacggcc gtcagcaaaa accacgcaat gatagtcatt
    12301 gaggatttga aggtcagcaa catgtcaaag tcagcagcgg gtacggtcag ccagccgggg
    12361 cgcaatgtcc gggcaaaatc aggtttaaa
//
LOCUS       DAEBXN010000115        12239 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.115, whole genome shotgun sequence.
ACCESSION   DAEBXN010000115 DAEBXN010000000
VERSION     DAEBXN010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12239
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.115"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..446
                     /locus_tag="KJL99_003389"
     CDS             <1..446
                     /locus_tag="KJL99_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HBD4437993.1"
                     /translation="SACAPETGLQQLVATPVPDEQRISFWPQHFGLIPQWVTLEPRVF
                     GWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGNRAEMSPEAAG
                     IAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            462..938
                     /locus_tag="KJL99_003390"
     CDS             462..938
                     /locus_tag="KJL99_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HBD4437994.1"
                     /translation="MQQLSFLPGNMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            1001..1222
                     /locus_tag="KJL99_003391"
     CDS             1001..1222
                     /locus_tag="KJL99_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HBD4437995.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            1385..1759
                     /locus_tag="KJL99_003392"
     CDS             1385..1759
                     /locus_tag="KJL99_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HBD4437996.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLYYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            1806..2180
                     /locus_tag="KJL99_003393"
     CDS             1806..2180
                     /locus_tag="KJL99_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HBD4437997.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFV
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            2177..2668
                     /locus_tag="KJL99_003394"
     CDS             2177..2668
                     /locus_tag="KJL99_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4437998.1"
                     /translation="MMKLALTLEADSVNVQALNIGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            2680..2877
                     /locus_tag="KJL99_003395"
     CDS             2680..2877
                     /locus_tag="KJL99_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4437999.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            2962..3522
                     /locus_tag="KJL99_003396"
                     /pseudo
     CDS             2962..3522
                     /locus_tag="KJL99_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            3594..4598
                     /locus_tag="KJL99_003397"
     CDS             3594..4598
                     /locus_tag="KJL99_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSfl8 family transposase"
                     /protein_id="HBD4438000.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKALLRKKFTRTKLMQFLAT
                     CPPSVVVMEACAGAHFMARCISDFGHETKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTETQQAMRALHRVRESLIRDKVKTTNQIHGFLLEFGISLPIGDAVIKR
                     LSLVLAEHEIPEYLSRLLVRLHTHYFYLVEQIAELESELSQSINADDTAQRIMTIPGV
                     GPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQYSTGGKSTLLGISKRGDKNLRRLL
                     VQCARSFIMRLERKHGKLAEWVREQLNKKHSNVVTCALANKLARIAWAITTQKKEYQA
                     "
     gene            4880..5161
                     /locus_tag="KJL99_003398"
                     /pseudo
     CDS             4880..5161
                     /locus_tag="KJL99_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(5311..5398)
                     /locus_tag="KJL99_003399"
     tRNA            complement(5311..5398)
                     /locus_tag="KJL99_003399"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5362..5364),aa:Ser,seq:gga)
     gene            5633..6571
                     /gene="ghrA"
                     /locus_tag="KJL99_003400"
                     /pseudo
     CDS             5633..6571
                     /gene="ghrA"
                     /locus_tag="KJL99_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
     gene            6626..7363
                     /gene="ycdX"
                     /locus_tag="KJL99_003401"
     CDS             6626..7363
                     /gene="ycdX"
                     /locus_tag="KJL99_003401"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HBD4438001.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMISTIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPLER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            7387..7941
                     /gene="ycdY"
                     /locus_tag="KJL99_003402"
     CDS             7387..7941
                     /gene="ycdY"
                     /locus_tag="KJL99_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HBD4438002.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMLL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            8043..8534
                     /locus_tag="KJL99_003403"
     CDS             8043..8534
                     /locus_tag="KJL99_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HBD4438003.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(8667..9373)
                     /locus_tag="KJL99_003404"
                     /pseudo
     CDS             complement(8667..9373)
                     /locus_tag="KJL99_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001571243.1"
                     /note="involved in the stability of the curlin proteins
                     during assembly; involved in the secretion of the major
                     curlin subunit CsgA across the outer membrane;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
     gene            complement(9400..9816)
                     /gene="csgF"
                     /locus_tag="KJL99_003405"
     CDS             complement(9400..9816)
                     /gene="csgF"
                     /locus_tag="KJL99_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HBD4438004.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            complement(9841..10230)
                     /gene="csgE"
                     /locus_tag="KJL99_003406"
     CDS             complement(9841..10230)
                     /gene="csgE"
                     /locus_tag="KJL99_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HBD4438005.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDSEKTVVFAL
                     IQTEEALNRRQINQALLSTDDLAHDEF"
     gene            complement(10235..10885)
                     /gene="csgD"
                     /locus_tag="KJL99_003407"
     CDS             complement(10235..10885)
                     /gene="csgD"
                     /locus_tag="KJL99_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HBD4438006.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            11638..12093
                     /gene="csgB"
                     /locus_tag="KJL99_003408"
     CDS             11638..12093
                     /gene="csgB"
                     /locus_tag="KJL99_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HBD4438007.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAVVVQRQSQMAIRVTQR"
     gene            12134..>12239
                     /locus_tag="KJL99_003409"
     CDS             12134..>12239
                     /locus_tag="KJL99_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin"
                     /protein_id="HBD4438008.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGG"
BASE COUNT         3198 a         2890 c         2983 g         3168 t
ORIGIN      
        1 cttcggcctg tgcaccggag actggcctgc aacaactggt tgccactccc gtccctgatg
       61 aacagcgcat cagcttctgg ccgcagcatt ttggcctcat tccacagtgg gtcaccctgg
      121 aaccccgtgt cttcggctgg atggaccgtc tgtgtgaaga ctactgcggt ggtatctgga
      181 atctgtacac cctgaacaac ggcggggcat ttatggcacc cgaaccggat gacgatgatg
      241 acgaaacatg ggtactgttc aatgccatga acggtaaccg cgctgaaatg agcccggaag
      301 ccgccggcat tgctgcctgt ctgatgacgt acagtcatca tgcctgtcgt acggagtgtt
      361 atgccatgac ggtccattat taccggctgc gggattacgc cctgcagcat ccggaatgca
      421 gcgccattat gcgcatcatc gactgaatgg aggcagggac aatgcagcag ctttcctttc
      481 tgcccggaaa catgacgccc ggcgagcgca gtctcattct gcgggccctg caaaccctgg
      541 accgccatct tcatgaaccc ggcgtggcct tcacctccac ccgtgcggca cgggaatggc
      601 tgattctgaa catggcggga ctggagcggg aagagttccg ggtgctgtat ctgaacaacc
      661 agaatcagct gattgccggt gaaaccctct tcaccggcac catcaaccgt acggaagttc
      721 atccccggga agtgattaaa cgcgccctgt accacaatgc cgctgccgtg gtgctggcgc
      781 acaatcaccc gtccggtgaa gtcacaccca gtaaggcaga ccggcttatc accgaacgtc
      841 tggtacaggc actgggcctg gtggatatcc gggtgccgga ccatctgata gtcggtggca
      901 accaggtttt ctcctttgcc gaacatggtc tgctttaacc cgtcacaacc acatcacacc
      961 tgttttcact tttatcttct gtcttcagag gtatcacatc atgagaatca tcacccgtgg
     1021 tgaagccatg cgtattcacc aacagcatcc tgcatcccgt ctttttccgt tctgtaccgg
     1081 taagtatcgc tggcacggca gtgctgaagc gtatactggt cgtgaagtgc aggatattcc
     1141 cggcgttctt gccgtgtttg cagaacgccg taaggacagt tttggcccgt atgtccggct
     1201 gatgagtgtc accctgaact gatgttccgt cacccgggaa acgagtacga ccagatgacc
     1261 tcagacgccg ctgtggatga ggccattctc atcaatgcat acatcttcac cgaaaccggt
     1321 cagcgcgccg gctgacctgt catcgttaaa ccattcttaa ccacttttca gagaggattt
     1381 tatcgtgtca gacaaactct ccgggataac tcatcccgat gacaatcacg accgcccctg
     1441 gtgggggctt ccctgcacag tgaggccctg ttttggcgct cgtctggtgc aagagggtaa
     1501 ccgcctgtat taccttgccg accgcgccgg tatcagaggc cgcttcagcg acgcagatgc
     1561 ataccatctg gaccaggcct ttccgctgct gatgaaacaa ctggaactca tgctcaccag
     1621 cggtgaactg aatccccgat atcagcatac cgtcacgctg tatgcaaaag ggctgacctg
     1681 cgaagccgac accctcggct cctgtggtta cgtttatctg gctgtttatc cgacaccagc
     1741 acccgcaacc acctcataac cccgaagctc tcacctttag cactaccagt tacagagaac
     1801 ttaagatgaa aacattatcc gacacacatg tacgggaggt atctcgctgc ccgtctcccg
     1861 tcaccatctg gcagacactg ctcatccgac tgctggacca gcattatggc ctcacactga
     1921 atgacacacc gtttgtcgat gaacgtgtga ttgagcagca tattgaggca ggcatttcac
     1981 tgtgtgatgc agttaatttt ctcgtggaaa aatacgcgct ggtgcgtacc gaccagccgg
     2041 gattcagcac ctgtccccgc tctcagttaa taaacagtat tgatatcctc cgggctcgcc
     2101 gggcgaccgg cctgatgacc cgcgacaatt acagaacggt aaataacatt accctgggta
     2161 agtatccgga ggcgaaatga tgaaactggc cctgacactg gaagccgaca gcgttaacgt
     2221 acaggcactg aacatagggc gcattgtcgt tgacgtcgat ggtattgagc tcgctgaact
     2281 gattaacatg gtctgcgata acggctactc ccttcgtgtt gttgatgaat ctgaccagac
     2341 ctcagcagaa tgcacgccac catttgctac ccttaccggc atacgctgca gtaccgcaca
     2401 tatcacggaa acggacaacg cctggttgta ctcgctgtca caccagacca gtgacttcgg
     2461 tgaatcagaa tggattcatt ttacaggtag cggatatctg ttacgtaccg atgcgtggtc
     2521 atacccggtt ctgcggctta aacgcctggg gctgtcaaaa acgttccgtc gtctggtcgt
     2581 cacactcatc cggcgttatg gcgtcagtct cattcatctg gatgccagcg ctgaatgcct
     2641 gccgggttta cccactttcg actggtaacc aggaacaaca tgaaatcatt aaccacggaa
     2701 acagcgctgg atattctgat tgcgtggctg caggacaata tcgactgcgg atcgggaatt
     2761 atctttgaca acgatgagga taaaacggat tcagcagcac tgttgccctg tatcgaacag
     2821 gcccgggagg atgtccgtac cctgcgtcat ctgcagcttc tgcaccagaa ccggtgaagc
     2881 tcactcacca gacttcattc ctctgacgcc agcctgcaca gggctggcgt tttcatttat
     2941 ctgcaaaaag gaatatcgat tatgtcagat atcacaatct cccgtccgga agtggtcaac
     3001 gggcatacgg acgttatctg ctcaaccagc attcgccaca ttctggctgt acgaaagagt
     3061 acactgttgc aaatcgacac acttatccga caactggctg aaatctcggt gctgacggaa
     3121 agcatcggag gtaaaactgc cccggactgg gccatgaaac aggattttcg ctgcggctgc
     3181 tggctgatgg agaaacccga aacagcaatg aaagccatta cccggaatct tgatcgcgaa
     3241 atctggcgtg acctgatgca acgttccggg atgctgtcat taatggatac gcaggcccgt
     3301 gatacatggt accggtcact ggagtacgat aattttccgg aaatcagcga agcgaacatt
     3361 ctgagcacat ttgaacaact gcaccagaat aaggatgagg tgtttgagcg aggggtgatc
     3421 aacgtcttca gagggctgaa ctggaattac aaaaccaatt gcccctgtaa atttggcagt
     3481 aaaattatcg tcaacaatct ggtgagatgg gaccggtggg gataatgata tggtcacccc
     3541 caccttaaca gcagtacgct gttaaggtaa atccctttaa cggagagacc atcatgcaga
     3601 acatcacact tattggtatc gatctcggaa agcactcctt ccacattcat tgtcaagaca
     3661 agtctggtaa ggctcttctg cgtaaaaaat ttacccgtac taaactgatg cagtttctgg
     3721 cgacatgtcc gccctcagtt gttgtaatgg aagcctgtgc gggtgcacac tttatggctc
     3781 gctgtatctc tgattttggg catgaaacta agctgatttc tccacagttc gttcgcccgt
     3841 ttgtcaaaag taataagaat gattttgttg atgcagaagc catatgcgaa gcggcttcac
     3901 gaccatcaat gcgttttgtc cagccaagaa ctgagaccca gcaggccatg agggcgttac
     3961 accgggtcag agaatcgctc atcagagata aggtgaaaac aaccaaccag atccacggtt
     4021 tcttgctgga atttggaatc agcctgccga taggtgatgc tgtaataaaa cggttgtctc
     4081 tcgtgttggc tgaacacgaa atccccgaat atctgagccg ccttttggtg aggttgcata
     4141 cccactactt ttaccttgtt gagcaaatag cagagctgga atcagaatta agtcagtcaa
     4201 tcaacgctga tgataccgct cagcgcatta tgacaattcc tggtgttggt ccgattactg
     4261 ccagcttact atcgtcgcaa cttggtgacg ggaaacagtt ctcatgcagc agggattttg
     4321 cagcttccac aggacttgtt ccccggcagt atagcactgg aggaaaatcg actctgttgg
     4381 gtatcagtaa gcgaggtgat aaaaatttac gccggttact ggttcagtgt gcccgctcat
     4441 tcataatgcg acttgaacgc aagcatggaa aacttgctga gtgggttcgg gagcaactca
     4501 acaagaagca ttcgaatgtg gttacctgcg ctctggcaaa taaactggct cgaattgcct
     4561 gggcaatcac tactcagaaa aaagaatatc aggcctgata tagatgctga tataactcaa
     4621 ttagtttatt ttaaacaata agatacaagt tacctttctg gttttgcgaa gactgattat
     4681 tgatgacatg aacggcaaat cgacctgatt aaaaacctaa cagatgaaat ggctcactga
     4741 agccgagggg atttttagga ttatcaggtg cggaactcat cggggcgcgg gcatccttgc
     4801 ccacagagac gccggataga tttaagcaag ccaaccatta atcaaaaatc agtgttgcaa
     4861 aaacgggggt gaccatagat ttcatcttat caccggacaa caggctgacc gactcgccga
     4921 tctggaaaga atgttgcacc tgttcagcgg taaaccaatc cccgacaacc ggggaaacat
     4981 cacccttcgt ctggatgatc acattcagtc tgttcagggt aaagagagct atgaagatga
     5041 gatgttcagc atcagatact ttaagaaggg ctccgcgcat atcacgttca gaaaaccaga
     5101 actggttgac agactgaatg atattatcgc aaaacactat cctgatatgc tggcagtata
     5161 gctttgccag tatgctcaaa gatcggaaat tgtccgataa gatatgatcg cggggcatat
     5221 aaaccggttt cagatggcat acccgatggc atactgtctg gaaggaaaaa taaaaaagcg
     5281 catgttaaac aataacatgc acttataatc tggcggtgag ggggggattc gaacccccga
     5341 tacgttgccg tatacacact ttccaggcgt gctccttcag ccactcggac acctcaccaa
     5401 attgtcgttc ctgtcttgct ggaacgggcg ctaatttagg gaaatcatga cctgaggtca
     5461 acaaactttt tgaaaaaatc gcgcgtttat tcaaacattc aatcaatgtg tggttttaat
     5521 aagcgaaatc tgcttttttt gccaccgacc acggatttgt tatgctggtg gcctttgtag
     5581 atcataacga taagtgcgaa taaatttcgc acaacgcttt tcgggagtca gtatggatat
     5641 catcttttat cacccaacgt tcgataccca atggtggatt gaggcactgc gcaaagctat
     5701 tcctcaggca agagtcaggg catggaaaag cggagataat gactctgctg attatgtttt
     5761 agtctggcat cctcctgttg aaatgctggc agggcgcgat cttaaagcgg tgttcgcact
     5821 cggggccggt gttgattcta ttttgagcaa gctacaggca caccctgaaa tgctgaaccc
     5881 ttctgttcca ctttttcgcc tggaagatac cggtatgggc gagcaaatgc aggaatatgc
     5941 tgtcagtcag gtgctgcatt ggtttcgacg ttttgacgat tatcgcatcc agcaaaatag
     6001 ttcgcattgg caaccgctgc ctgaatatca tcgggaagat tttaccatcg gcattttggg
     6061 cgcaggcgta ctgggctgta aagttgctca gagtctgcaa acctagcgct ttccgctgcg
     6121 ttgctggagt cgaacccgta aatcgtggcc tggcgtgcaa agctttgccg gacgggaaga
     6181 actgtctgca tttctgagcc aatgtcgggt attgattaat ttgttaccga atacccctga
     6241 aaccgtcggc attattaatc aacaattact cgaaaaatta cctgatggcg cgtatctcct
     6301 caacctggcg cgtggtgttc atgttgtgga agatgacctg ctcgcggcgc tggatagcgg
     6361 gaaagttaaa ggcgcaatgc tggatgtttt taatcgtgaa cccttaccgc ctgaaagtcc
     6421 gctctggcaa catccacgcg tgacgataac accacatgtc gccgcgatta cccgtcccgc
     6481 tgaagctgtg gagtacattt ctcgcactat tgcccagctc gaaaaagggg agagggtctg
     6541 cgggcaagtc gaccgcgcac gtggctacta ataaagcatc aggattcctg ctatccttgg
     6601 cgggaattga atacaggaga gagttatgta tcccgtcgac cttcatatgc ataccgttgc
     6661 cagcacacat gcatatagca cattgagtga ttacattgcc caggccaaac aaaagggcat
     6721 taaacttttt gcgatcaccg atcatggccc ggatatggaa gatgcgccgc atcactggca
     6781 cttcattaac atgcgtatct ggccgcgagt ggttgatggg gtagggatcc tgcgcggcat
     6841 tgaagctaac attaaaaatg ttgatggtga aattgactgc agcggtaaaa tgtttgactc
     6901 gctggatctg attattgccg gttttcatga gccggttttt gcgccacatg acaaagcgac
     6961 caatacacaa gcgatgatct ccactatcgc cagcggcaat gtgcatataa taagccatcc
     7021 cggaaatccg aaatatgaaa tagatgttaa agccgttgct gaagcagccg cgaaacatca
     7081 ggtggcgctg gaaatcaata attcctcatt tttacactca cgtaagggca gtgaagacaa
     7141 ctgtcgtgcg gtagctgcag cggtacgtga tgctggaggt tgggtggcat taggctcgga
     7201 ttctcacacc gcgtttacca tgggggaatt tgaagagtgt cttaaaatcc tcgacgcggt
     7261 agattttccg ctagagcgca ttttgaatgt ttctccgcgc cgcttactga acttccttga
     7321 atctcgcggt atggcaccga ttgcggaatt tgcagacctt taattactag taatggaaat
     7381 atattaatga acgagttttc tatcctctgt cgtgtgctgg gttcgctcta ttaccgccaa
     7441 ccgcaagatc ctttactggt gccgctgttt actctgattc gtgaggggaa actggctgcg
     7501 aactggccac tggagcagga tgagttactg acgcgtttac agaaaagctg tgatatgacg
     7561 caagtctctg ccgattacaa tgcgttgttt atcggcgatg aatgtgccgt gccgccatat
     7621 cgtagcgcat gggttgagga cgcaacggaa gcggaagtgc gcgcttttct ttccgaacga
     7681 gggatgctac tggcggatac gccagccgat cacatcggca cattgctgct tgcggcttcc
     7741 tggctggaag atcagtcaac ggaagatgag agcgaagcac tggaaacact gttcagtgag
     7801 tatctgttac cctggtgtgg tgcgttcctc ggcaaagtgg aggcccatgc aaccacgcct
     7861 ttctggcgca ccatggcacc gctaacccgt gatgccatta gtgcaatgtg ggacgagctg
     7921 gaagaagatt ctgaagagta aatgtgatca tcgtcactca taactgcaac agataatttg
     7981 ttgttgcata aaatgtgtgc tcgatctcat tcatggccgc gttttctgct atcatgcgcg
     8041 gcatgaacat acttctctct attgcaatca caacaggcat tctctccggt atctggggat
     8101 gggtggctgt ttccctgggc ttactaagct gggcgggctt cctgggctgt acggcctact
     8161 ttgcttgccc gcaaggtggg ctgaaagggc tggcgatctc cgctgcaacc ctgcttagtg
     8221 gcgtggtgtg ggcgatggtc attatttacg gtagtgcact ggcaccacat ctggaaattc
     8281 tcggttatgt cataaccggt attgtcgcgt ttctgatgtg tattcaggcc aaacagctgc
     8341 tgctttcatt tgttccgggg acgtttatag gcgcatgcgc gacgtttgcc gggcagggtg
     8401 actggaaact ggtgttacct tcgctggcgc ttgggctggt atttggttac gcaatgaaaa
     8461 acagcggcct gtggctggcg gcgcgtagtg caaagacggc tcaccgtgag caggaaatca
     8521 aaaataaagc gtgaggggca ctcacgcttt cgcttaaaca gtaaatgccg gattattaat
     8581 tccggcattt ttatctgtca ggattccggt ggaaccgaca tatggcggta tttcaccaga
     8641 atgtcattct gccgttctgc tttattttac aaatcccaca gaccacggtc gataccatca
     8701 ttaatcagga aaatgacccc tgtttcgata gccgacatca gacacagcat aacgggttcg
     8761 tttgaggtgt aacccacttc cccttccagc aagcgctggt agtcaataaa gcggaaaacc
     8821 ccggcctgaa cttcatagga aagtatcgtc ttactggtgt tcaccgagga gaggatctca
     8881 ccggtactca cattgacgac acgcaggttc acggcaatct gatcgagctg gtattgcgtg
     8941 tctgcaccga tcccaaaata tcttgcccca acaccgcccg atttaacgtt gctttcataa
     9001 ccgataatcg aaccttcaac cataatattt gccgccgtta aagattgcag cgggattcgg
     9061 ttattaatgg caaccgtgcc gttttcttgt gccgcacgaa taaccttgcg ctcgttaagc
     9121 aggttttgta agccctggcg ctttgcggaa cagcagtgga gaagttactt gccgggtagg
     9181 gtttaaattg cccggtttcg tcctgaatgt tgtataccga aacaaagatc ttacccgtcg
     9241 gcgctggcag atgggtcaaa tctttgtagc tctgagcacg aggcattaat gtcggtctgg
     9301 cggcttcttt aggcggggcg gttaagcatc cgctcagtaa catgacggca accaaaagaa
     9361 ataagcgctg catgattatt ttccttatga agctggggct taaaaatcgg ttgagttatt
     9421 ttgtaaaccc gaaacctgga tggtcgaggt ttgtccggtt ttacgatctg tcacgttcaa
     9481 ctgcaattga ccatcgcggt tagcaatatc aacaatataa tcgttggtca ccatgcggcc
     9541 cggtttaccg gtattaatat tcgacagtag cccacctaaa atttgtgact ggatggcctg
     9601 agtaaagtta tctaacgctg agggtgtttc aataccaaag tcatcgttat agctcggatc
     9661 tttataagag ttttgggcct gagcgctatt taataaaaaa gcgccattat ttgggttacc
     9721 accaaagttt ggattacgga actggaaagt catggttcca gcccaactta atggcgaaat
     9781 aagcatgagt agaactactg catgtttgac acgcattgca gcctccgaac aattttttat
     9841 ttagaattca tcatgcgcca aatcgtccgt acttaataac gcctgattta tctggcgacg
     9901 atttagtgct tcttcagttt gaatcagtgc aaagacgaca gttttctcgg agtctctttt
     9961 caacgggaat aaaaaagtct ggaaaataac atcctgattg accgttattg tgatccagct
    10021 tccccatcgt gcactgggcc tttcattaat cgttaagtta cccgtatagt cactttccca
    10081 tttatcacta aaggctcggt aaaaatcatg gccaatagat gaaacagtat ggtcagttaa
    10141 caatcccggg acttctacct caacggcgtg aagattccct gcggcgaaca gaaattctgc
    10201 cgccacaatc cagcgtaaat aacgtttcat ggctttatcg cctgaggtta tcgtttgccc
    10261 aggaaaccgc ttgtgtccgg ttttttacgg ctatcttctt gaaaagatta taaagatgcg
    10321 ttttaaccgt attttcgctg atgaacaacg aacgagcgat ctcgttatta gacgcgccga
    10381 tacgcagctt attcaggatc tctttttccc gatgagtaag gagggctgat tccgtgctgt
    10441 tataacgata gttaccagaa tgtgtaatca ggtaactggc aagcttttgc gtaaagtagc
    10501 attcgccacg caggacgcct tgcaacccat tgacaacacg ttcttgatcc tccatggcat
    10561 aaaaaacgcc gttgatatga ggccagtttt caatgtcgcg gtacgggtaa tcttcaggcg
    10621 tatttagcaa caatattttg atattgttgt ttttcctgct caaagtatcc tgccaataat
    10681 ggataagctt tttatccgct tccatcatat ccagaagaat aatagagcct gaagagatat
    10741 cgtccagaga acgttgaata ttatgtaatt ttcctgtaat tgctagcgat tgtttaaggt
    10801 gctgcaagag agctgtcgcc tgcaaagaag gtttagtgat caacaataat gtatgaccat
    10861 gaatactatg gacttcatta aacatgatga aaccccactt ttttattgat cgcacacctg
    10921 acagctgcct ctaaaataga agcaccagaa gtactgacag atgttgcact gctgtgtgta
    10981 gtaataaatc agccctaaat gggtaaaata taaaactaat ggattacatc tgatttcaat
    11041 ctagccatta caaatcttaa atcaagtgtt aaacatgtaa caaaatgtaa ctcattatat
    11101 taaaatgtta acattaaggt tttattaagt ttagaaatga tagaaaagtt gtacatttgg
    11161 tttttattgc acaattttaa aaaatcatac aaatggtgct aacttactaa taatacatat
    11221 aaaaaatatt tcggtgtagt cctttcgtca tgtaaaacgt ttttgttttt tctccacacc
    11281 tccgtggaca attttttact gcaaaaagac gaggtttgtc acggcttgtg cgcaagacat
    11341 atcgcagcaa tcagcgacgg gcaagaagaa tgactgtctg gtgatttttg atagcggaaa
    11401 acggagattt aagagaaaac aaaatatttt ttgcgtagat aacagcgtat ttacgcgggt
    11461 tttaatactt tggtatgaac aaaaaaagaa aaatacaacg cgcgggtgag ttattaaaaa
    11521 tatttccgca gacatacttt ccatcgtaac gcagcgttaa caaaatacag gttgcgttaa
    11581 caaccaagtt gaaatgattt aatttcttaa atgtacgacc aggtccaggg tgacaacatg
    11641 aaaaacaaat tgttatttat gatgttaaca atactgggtg cgcctgggat tgcagccgca
    11701 gcaggttatg atttagctaa ttcagaatat aacttcgcgg taaatgaatt gagtaagtct
    11761 tcatttaatc aggcagccat aattggtcag gctgggacta ataatagtgc tcagttacgg
    11821 cagggaggct caaaactttt ggcggttgtt gcgcaagaag gtagtagcaa tcgggcaaag
    11881 attgaccaga caggagatta taaccttgca tatattgatc aggcgggcag tgccaacgat
    11941 gccagtattt cgcaaggtgc ttatggtaat actgcgatga ttatccagaa aggttctggt
    12001 aataaagcaa atattacaca gtatggtact caaaaaacgg cagttgtagt gcagagacag
    12061 tcgcaaatgg caattcgcgt gacacaacgt taattaccat tcgactttta aatcaatccg
    12121 atgggggttt tacatgaaac ttttaaaagt agcagcaatt gcagcaatcg tattctccgg
    12181 tagcgctctg gcaggtgttg ttcctcagta cggcggcggc ggtaaccacg gtggtggcg
//
LOCUS       DAEBXN010000116        12220 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.116, whole genome shotgun sequence.
ACCESSION   DAEBXN010000116 DAEBXN010000000
VERSION     DAEBXN010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12220
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.116"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(<1..493)
                     /locus_tag="KJL99_003410"
     CDS             complement(<1..493)
                     /locus_tag="KJL99_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBD4438009.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSA"
     gene            complement(524..1216)
                     /gene="fimC"
                     /locus_tag="KJL99_003411"
     CDS             complement(524..1216)
                     /gene="fimC"
                     /locus_tag="KJL99_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HBD4438010.1"
                     /translation="MMTKIKLLMLMIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIKNSHTNELFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(1436..1978)
                     /gene="fimA"
                     /locus_tag="KJL99_003412"
     CDS             complement(1436..1978)
                     /gene="fimA"
                     /locus_tag="KJL99_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HBD4438011.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIVSSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            2459..3325
                     /gene="folD"
                     /locus_tag="KJL99_003413"
     CDS             2459..3325
                     /gene="folD"
                     /locus_tag="KJL99_003413"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HBD4438012.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            3327..3539
                     /gene="ybcJ"
                     /locus_tag="KJL99_003414"
     CDS             3327..3539
                     /gene="ybcJ"
                     /locus_tag="KJL99_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HBD4438013.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            3647..4156
                     /locus_tag="KJL99_003415"
     CDS             3647..4156
                     /locus_tag="KJL99_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBD4438014.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKGLLFAGIILAMLPDADVLSFKFG
                     VAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSLLDSV
                     TTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLPGMLL
                     MGMLWWRRR"
     gene            complement(4192..5577)
                     /gene="cysS"
                     /locus_tag="KJL99_003416"
     CDS             complement(4192..5577)
                     /gene="cysS"
                     /locus_tag="KJL99_003416"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HBD4438015.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNSDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNRMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            5751..6245
                     /gene="ppiB"
                     /locus_tag="KJL99_003417"
     CDS             5751..6245
                     /gene="ppiB"
                     /locus_tag="KJL99_003417"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HBD4438016.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRNGMHQDVPKEDVIIESV
                     TVSE"
     gene            6248..6970
                     /gene="lpxH"
                     /locus_tag="KJL99_003418"
     CDS             6248..6970
                     /gene="lpxH"
                     /locus_tag="KJL99_003418"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HBD4438017.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            7088..7597
                     /gene="purE"
                     /locus_tag="KJL99_003419"
     CDS             7088..7597
                     /gene="purE"
                     /locus_tag="KJL99_003419"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HBD4438018.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAVEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNGWRKAQTDEVL
                     ENPDPRGAA"
     gene            7594..8661
                     /gene="purK"
                     /locus_tag="KJL99_003420"
     CDS             7594..8661
                     /gene="purK"
                     /locus_tag="KJL99_003420"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HBD4438019.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(8798..9691)
                     /locus_tag="KJL99_003421"
     CDS             complement(8798..9691)
                     /locus_tag="KJL99_003421"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HBD4438020.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(9688..10335)
                     /locus_tag="KJL99_003422"
                     /pseudo
     CDS             complement(9688..10335)
                     /locus_tag="KJL99_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
     gene            10338..11570
                     /gene="allC"
                     /locus_tag="KJL99_003423"
     CDS             10338..11570
                     /gene="allC"
                     /locus_tag="KJL99_003423"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HBD4438021.1"
                     /translation="MTHSRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFKK
                     RMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWLA
                     IDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNSF
                     ADAMKACGFTLPNAPLTPRQAIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTVT
                     LNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNVV
                     PGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKELV
                     ATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITDL
                     AEGVKTLALMLYQLAWQE"
     gene            11581..>12220
                     /gene="allE"
                     /locus_tag="KJL99_003424"
     CDS             11581..>12220
                     /gene="allE"
                     /locus_tag="KJL99_003424"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HBD4438022.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLIKNIIPDFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLFSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVY"
BASE COUNT         2966 a         3158 c         3118 g         2978 t
ORIGIN      
        1 gagcgctact caacaacgct atctgaggaa tagtgatgtt caggcgcatc gctgcaaaat
       61 caaagttaat tgtggcatca ggtacagctt tcagtaaatt gatgcattta ttgttttgct
      121 gctgggctaa ctcgggaaat gcactgctat taaggccaat tcgttcaagg agtacctggt
      181 taaaacaggg cattaaccca cctgatttat cacctgtatt ttctgttgtc gattcaaata
      241 cgatatcctg tgaaccaatg aactcatcat tacgccagag atccacccga tacacgcccg
      301 caggttgatg attacctctc tcaaagcgcg ataaatcagc aacggatgtg ccattctcta
      361 ataaaaatgc cgggttgaaa taactttccg catagcttaa aaaggagggt aaaatggcaa
      421 gagatgaata acaaattccg gccagacacc aggtatacct ctgcggaata tccgtagtag
      481 tgggtatttt cataattagc tctgatattg tcagatgatt cggttagttt aagttcactt
      541 ctctgaccgg ggttactgaa ccataatcat taacggtctg aaatttcagc tttccctgca
      601 ctcctgaggg taagggaatt tgtgaactaa ctctgggtgc cgccatagca ttccccaact
      661 tttggctgcc cacttgcagg ttaacgaggg tgacataaaa aggtgatgga ttatgaacat
      721 tgatatagtt accggatcgc gaaaacttga gtgtgtccgg cgcttctgcg ggtaattctt
      781 gtaattgaat tggacggaga aataatttca tgcgcgataa aatcgccagt tgcaaaacat
      841 tcctgccgtt caatgcattt ttatctaccg aagggatcgt cttaacattc atccagaaca
      901 gagactcacg atctgccgcc agcggtggac cggtgtaaat aatacgcaaa gtattttcgc
      961 ttttgggttc actaacaaat agtggcggtg taatgatgaa tgacttttct tttacaccgc
     1021 tgctgttttc aatccacgaa ttgaccagaa agagctcatt ggtatggcta tttttaatcc
     1081 ataccgcagt ctgtttagca tcagcgggat aaataatacg cgtggcacct aatgcgatcc
     1141 ctccggcagc atgggcgctg gccgaaatga ttaaataaaa tatcatgagc atcaataact
     1201 ttattttagt catcatagtt aatacgcctt gtgtattcct gacggcaaaa ataaggagta
     1261 taaagaaatg attgctttgg cttcttacaa cacatcattc ccttttcatt taataaagcg
     1321 gcatgataca aagtgaacgt ggtttcgatt tacattgcca ccataccgct tcagacaacc
     1381 ggtacattac tttgttataa gacgtgatat ataattcaaa acaacgtggt tttgattatt
     1441 cgtatctcat aataaaagtc gcgtcagcat ttgcttgccc tgctgatgca cttgtaccgg
     1501 tgcctttata acgtgcagaa aaatgaagaa cgttggtccc ggggatcaag ttctggttgg
     1561 ttgagaagct attcccatca ggtttgagaa ttgcggatgt attatcaagt atttcaatac
     1621 cgacacccgt tgctgttgtt gtatttgtac tggatacaat ggccaataaa ttatcattga
     1681 ttgcatcagc ctgaccagaa aatgcaacgg cagcattagc ggcaacaaca ggatcgcagt
     1741 cgttcaactg aatggtgaat ggaattaatg cagaggtatt accaacagca ttgaaaatat
     1801 cggtacgata ttgaccgagt gtgacaacct ggtctgccga gtcagtattc actgaacagg
     1861 cagcattcac cagttcgcct tcaaagtgaa ttgtaccgcc atctacaacg gcagcataac
     1921 tacccgtagc ggcgaatagt gcggcagcca gcgcagacga aataaatctt aatttcatat
     1981 atattccttc aatctcattt atcgactcca catccgtata taaccgatta ctttatttaa
     2041 gacactgata gtagtaaaat tcctttttat cctctaagaa tgtcttaatt gaaaatatgc
     2101 actctattct aaaaaataga gagccccgtt agatgaatac ttccgcgcaa aatatattca
     2161 ccacaaatat agacctgaag cggtaaatta ccaggctgaa aattcttttt atattgtcag
     2221 gtatttctta aattatctta atccttaaac aaggaaataa atcagttcca gatttacaac
     2281 gccatcatgt acgaaaaatg aagctttcaa tctcagcgac ctcagcgacg gtgcgcctaa
     2341 ccttcgcaag aggtcgcttc acgcgataaa tctgaaacga aacctgacag cgcgccccgc
     2401 ttctgacaaa atagacgcat ccccttcgac ctacgtaaca gatggaatcc tctctctgat
     2461 ggcagcaaag attattgacg gtaaaacgat tgcgcagcag gtgcgctctg aagttgctca
     2521 aaaagttcag gctcgtattg cagccggact gcgggcacca ggactggccg ttgtgctggt
     2581 gggtagtaac cctgcatcgc aaatttatgt cgcaagcaaa cgcaaggctt gtgaagaagt
     2641 cgggttcgtc tcccgctctt atgacctccc ggaaaccacc agcgaagcgg agctgctgga
     2701 gcttatcgat gcgctgaatg ccgacaacac catcgatggc attctggttc aactgccgtt
     2761 accggcgggt attgataacg tcaaagtgct ggaacgtatt catccggaca aagacgtgga
     2821 cggtttccat ccttacaacg tcggtcgtct gtgccagcgc gcgccgcgtc tgcgtccctg
     2881 caccccgcgc ggtatcgtca cgctgcttga gcgttacaac attgatacct tcggcctcaa
     2941 cgccgtggtg attggcgcat cgaatatcgt cggtcgccca atgagcatgg aactgttgct
     3001 cgcaggttgc accaccaccg tgactcaccg cttcactaaa aatctgcgtc atcacgtaga
     3061 aaatgccgat ctgttgatcg ttgccgttgg caagccaggc tttattcccg gtgactggat
     3121 caaagaaggc gcaattgtga ttgatgtcgg catcaaccgt ctggaaaatg gcaaagttgt
     3181 gggcgacgtc gtgtttgaag acgcggctaa acgcgcctca tacattacgc ctgttcccgg
     3241 cggcgttggc ccgatgacgg ttgccacgct gattgaaaac acgctacagg cgtgcgttga
     3301 atatcatgat ccacagggtg agtaacatgg cgacattttc tttaggtaaa catccgcacg
     3361 ttgagctgtg cgacttgctg aaactggaag gctggagcga aagcggcgcg caggcgaaaa
     3421 tcgcgattgc cgaaggccag gtgaaagtcg acggtgcggt tgaaacgcgc aaacgctgca
     3481 aaatcgtcgc cggtcagaca gtgagttttg caggacacag cgtacaggtt gttgcctgat
     3541 ccctttcccg gcctgtatca acaggccggt tctcttttca gacttttgcc ctctacgccc
     3601 cgttcatcca tgctgatagc atcgacattt tgccgaagcg accatcatgc caaccgttat
     3661 tacgcacgcc gccgttcccc tttgcattgg tttagggttg ggttcgaaag gtctgttatt
     3721 tgccggaatc atcctggcga tgctgcccga tgccgacgta ttgtcgttta aatttggcgt
     3781 tgcttacggc aatgtttttg gtcatcgcgg ttttacccat tcgctggtgt ttgcgtttgt
     3841 tgtcccgctc ttatgtgtgc tgattggacg tcgatggttc agggctgggc tgattcgctg
     3901 ctggctgttt ttaaccgtct cgttgctatc gcacagctta ctggattcgg taaccactgg
     3961 cggtaagggc gttggctggc tgtggccgtg gtcagatgaa cgctttttcg ctccctggca
     4021 ggtgattaaa gtcgcgccgt ttgcattgtc tcgttacacc acagcgtacg ggcatcaggt
     4081 gattatttcc gaattgatgt gggtgtggtt gccggggatg ctactgatgg gaatgttgtg
     4141 gtggcgcaga cgataaccgg atgcgaaaac tcgcatccgg caattgcgca attacttacg
     4201 acgccaggtg gtcccttgcg ggccatcttc cagcacgatc cccatctcgt taagacggtc
     4261 acgtgccgca tctgccgccg cccagtcttt cgctttacgg gcatccagac gctgttgaat
     4321 taacgcttca atctcagcca cttcgctgtc gtctgcctgc gcgccgcttt gcaggaacgc
     4381 ttccggttct tgctccagca ggcccaatac agcggaaagt ttacgcaggt gagatgccat
     4441 tgcattcgcc gctgccatat cttctgcttt cagacggttc acttcacgcg ccatatcaaa
     4501 cagcacggaa taggcttccg gggtgttgaa atcgtcgtcc atcgcttcaa taaagcgcgc
     4561 ttcaaacgct tcgccaccgg caggcgcaac ggttttatct gtgccgcgca gcgcagtgta
     4621 gaggcgctcc agcgccgcac gcgcctgctt caggttctct tcgctgtagt tcagctggct
     4681 gcgatagtgg cccgacatca ggaagtaacg cacggtttcc gcgtcgtagt atttcagcac
     4741 atcgcgcacg gtaaagaagt tacccagcga tttggacatc ttctcgcggt caaccatcac
     4801 cattccggag tgcatccagt agttcacata ctgaccatca tgggcacagg tggactgcgc
     4861 gatttcgttc tcgtggtgcg ggaacatcag gtctgaaccg ccgccgtgga tatcaaagtg
     4921 gttacccagc tgcttgcagt tcatggccga acattcaatg tgccagccag gacgacccgc
     4981 gccccacgga gacggccagc tcggttcgcc ctctttcgac atcttccaca gaacgaagtc
     5041 catccggttg cgtttgtcat caaccacgtc aacgcgcgcg ccagcctgca gctggtcgag
     5101 atcctgacgc gacagcacgc cataagttgg atcggtcggg acgtcgaaca tcacgtcgct
     5161 gttgtccgcc acataagcgt gacctttggc gatcagttgt tcagtgagtt caataatttc
     5221 tgcgatatgg tgcgtcgcgc gcggctccat atccgggcgc agaatgttca aagcatcaaa
     5281 atctttgtgc atttcggcga tcatgcgatc caccagcgcc acaaagcttt cgccattttc
     5341 attggcgcgt ttgatgattt tgtcgtcgat atcggtaatg ttgcgcacat acttcagttt
     5401 atagccgagg aaacgcagat agcgcgcaac cacgtcaaaa gcaacaaagg tacgcccgtg
     5461 accgatatga cagagatcgt aaacggtgat tccacacacg tacatgccga cttccccggc
     5521 gtgaataggc ttaaattcct ctttttggcg tgtcagagta ttgaagattt ttagcatcga
     5581 agattccgtt tagacatgtg tgggtaattg agttgcgtat aatacccata tttcccgcgc
     5641 gaatcagcat acattgcgtg atgatccgat cgtgcggtta tgctataaca ccaccctata
     5701 tatgacccga actgggttga agcaccaatc aaacggaaca ggatgcaaaa atggttactt
     5761 tccacaccaa tcacggcgat attgtcatca aaacttttga cgataaagca cctgaaacag
     5821 ttaaaaactt cctggactac tgccgcgaag gtttttacaa caacaccatt ttccaccgtg
     5881 ttatcaacgg ctttatgatt cagggcggcg gttttgaacc gggcatgaaa caaaaagcca
     5941 ccaaagaacc gatcaaaaac gaagccaaca acggcctgaa aaatacccgt ggtacgctgg
     6001 caatggcacg tactcaggct ccgcactctg caactgcaca gttcttcatc aacgtggttg
     6061 ataacgactt cctgaacttc tctggcgaaa gcctgcaagg ttggggctac tgcgtgtttg
     6121 ctgaagtggt tgacggcatg gacgtggtag acaaaatcaa aggtgttgca accggtcgta
     6181 acggtatgca ccaggacgtg ccaaaagaag acgttatcat tgaaagcgtg accgttagcg
     6241 agtaatcgtg gcgacactct ttattgcaga tcttcatctc tgcgtggaag aaccggcgat
     6301 caccgccggt tttctgcgtt ttttagcggg ggaagcccgc aaggccgacg cgctgtatat
     6361 tcttggcgat ctgtttgaag catggattgg cgacgacgat cccaacccac tccatcgcca
     6421 gatggcggca gcgatcaaag cggtgtccga ttccggcgtt ccctgttatt tcattcatgg
     6481 caaccgtgat tttctgctcg gcaaacgctt tgcccgtgaa agcggcatga cattattgcc
     6541 ggaagaaaag gtgctcgaac tttatggtcg ccgggtgttg attatgcatg gcgacacgct
     6601 gtgcaccgat gacgcgggtt atcaggcttt tcgcgccaaa gtccacaaac cctggctgca
     6661 aatgctattc ctcgccctgc cgttgtttgt gcgcaaacgc attgccgcgc gaatgcgcgc
     6721 gaacagcaaa gaagccaaca gcagtaaatc gctggcgatc atggacgtta accaaaacgc
     6781 tgtggtcagt gcgatggaaa aacatcaggt gcaatggcta atccacgggc atacccatcg
     6841 cccggcggtg catgaactta tcgccaacca gcaacctgct tttcgcgtgg tactgggtgc
     6901 ctggcatacg gaaggttcga tggtgaaagt cacggctgat gacgttgagc tgattcattt
     6961 tccgttttaa aaaacccaca actttgctga tttcacagcc acgcaaccgt tttccttgct
     7021 ctctttccgt gctattctct gtgccctcta aagccgagag ttgtgcacca caggagtttt
     7081 aagacgcatg tcttcccgca ataatccggc gcgtgtcgcc atcgtgatgg ggtccaaaag
     7141 cgactgggct accatgcagt tcgccgtcga aatcttcgaa atcctgaatg tcccgcacca
     7201 cgttgaagtg gtttctgctc accgcacccc cgataaactg ttcagcttcg ccgaaagcgc
     7261 cgaagagaac ggttatcagg tgattattgc gggcgcaggc ggcgcagcgc acctgccagg
     7321 catgattgcc gccaaaacgc tggtgccggt gctgggcgtg ccagtacaga gcgccgcact
     7381 gagcggtgtc gatagcctct actccatcgt acaaatgccg cgcggcattc cggtgggtac
     7441 gctggcgatt ggtaaagctg gcgcggcaaa cgcggcatta ctggcagcac aaattctcgc
     7501 gactcacgat aaagagctac accagcgtct gaatggctgg cgcaaagccc agaccgacga
     7561 agtgctggaa aacccggacc cgcgaggtgc ggcatgaaac aggtttgcgt cctcggtaac
     7621 gggcagttag gccgtatgct gcgtcaggca ggcgaaccgt taggcattgc tgtctggccg
     7681 gtcgggctgg acgctgaacc ggcggcggtg ccttttcaac aaagcgtgat taccgctgag
     7741 atcgaacgct ggccggaaac cgcattaacc cgcgagctgg cgcgtcatcc ggcctttgtg
     7801 aaccgcgatg tgttcccgat tattgctgac cgtctgactc agaaacagct tttcgataag
     7861 ctccacctgc cgaccgcgcc gtggcagtta cttgccgatc gcagcgagtg gcctgcggtg
     7921 tttgatcgtt taggtgaact ggcgattgtt aagcgtcgca ctggtggcta tgacggtcgc
     7981 ggtcaatgga gtttacgcgc caatgaaacc gaacagttac cggcagagtg ttacggtgaa
     8041 tgtattgtcg agcagggcat taacttctct ggtgaagtat cgctggttgg cgcgcgcggc
     8101 tttgatggca gcaccgtgtt ttatccgctg acgcataacc tgcatcagga cggtattttg
     8161 cgcaccagcg tcgcttttcc gcaggccaac gcacagcagc aggcgcaagc cgaagagatg
     8221 ctgtcggcga ttatgcagga gctgggctat gtgggcgtga tggcgatgga gtgttttgtc
     8281 accccgcaag gtctgctgat caacgaactg gctccgcgtg tgcataacag cggtcactgg
     8341 acacaaaacg gtgccagcat cagccagttt gagctgcatc tgcgggcgat taccgatctg
     8401 ccgttaccgc aaccagtagt gaataatccg tcggtgatga tcaatctgat tggtagcgat
     8461 gtgaattatg actggctgaa attgccgctg gtgcatctgc actggtacga caaagaagtc
     8521 cgtccggggc gtaaagtggg gcatctgaat ttgaccgaca gcgacacatc gcgtctgact
     8581 gcgacgctgg aagccttaat cccgctgctg ccgccagagt atgccagcgg cgtgatttgg
     8641 gcgcagagta agttcagtta agcaggtgct catttgattg ccggatgcgg cgtaaacgcc
     8701 ttatccggct taccgctccg gtacccattg taggcctgat aagacgcgtc aagcgtcgca
     8761 tcaggcatgg tgcaccgatt gccggatgct ctcatcacta cagcgaaata caggtccccg
     8821 cttcgcccgc cagcgtctct tcaattcgcg ataacgcccc aatccacgcg ggtttactac
     8881 ggcttctgac ataaccactc accgccgtta ccttcggccc catcgaacca tcggctttgg
     8941 caaatggcgc taactcatcc ggtgtggcat ggcgaatggc acgttgctgc ggcgttcccc
     9001 agttttcata taccgcatca gcatcggtga ggatcaccag tccatcggca ttaatctgct
     9061 cggcgagcaa cgcagcggcg agatctttat caatcaccgc ttcactccct gccccatctt
     9121 ccgtcacagg cacaccgccg ccgccactgc aaatcaccac atgcccctct ttgagcaaca
     9181 actcgatggc ttcgctgtcg agaattttac gcggttgcgg agacgccacc acccggcgca
     9241 aatatttacc atcacgtttc atctgccagc cgtaagccgc ttccagtgcc tcttgttctt
     9301 ctggctgata aaccggacca ataaatttct ctggctgcaa aaacgccgga tcatcaggcg
     9361 aaacctcaat gcgcgtcagc accgtcgtaa caggcggcat ctgcggctgt gcgctcaagc
     9421 tctgcgccag catatagcca atcatcccct ggctttccgc caccagtaca tccagcggat
     9481 acggttctac ctctttccac gccagattct gtaacgccag cagccccacc tgcggcccgt
     9541 tgccgtgaac aatcgccagc cgataagaac gggccaggcg tgccagcgcg ggtacagcac
     9601 tggcgatatt gcgatattga ttttccgccg tcagcgcctc accgcgctgg agtaaggcgt
     9661 tgcccccaag agctacaacc agtgttttca tggttttcct tgtaataatt gttggccaag
     9721 ccagaacccc agcagcgtat ctgcccctga cgtatgcccc agcgccagca gcgaatcaat
     9781 cgcaacagcg gtacgtttcg ggcaactcag agcatggaca aagtgcagga gtggcgaggc
     9841 gaaatatcct tgcgcggcat aacgtaaata actgacgctc accgctgtgg taacgagttg
     9901 aagattgtcg gaacaggcaa aaaacgggcg accggcgcgc gcatctaaag cgccataata
     9961 ccaggccgcc agcagcattc cgctcaacgt gtcatcatga cttggcgtta atccggggcc
    10021 tttacccagc cagtgccgcc agtcggtctt aacgccattg agcgcggcct gaaaacagtg
    10081 acgaaactgg cgtaattcag cgggcagcgg atcgcttgcc gccatcgcca gtggcccgaa
    10141 aagtccggtt tcctccgcgc gttgcatcca tgcagctgca agcggttgag gatgcgaagg
    10201 cggcgtaata cgcagcaaac aataacgctg tggctgttta accgtgaaac gcccgaggcg
    10261 aatcccttga gccacaacct gtggtcgttc attgccgcat aatccaccgc ataacgcatc
    10321 gaattgcgtg cggcgaaatt acacattccc gtcaagctat agaagaaacg ctgccctggc
    10381 tttcctcttt tggcgctgac ccaacgggtg ggatgacccg tttactttat tcgccggaat
    10441 ggctggaaac ccagcagcaa tttaaaaaaa gaatggcagc aagtgggctg gaaacacgtt
    10501 tcgatgaagt ggggaattta tacggtcgcc tgagtggcac cgaatatcca caggaagtgg
    10561 ttctgagcgg ttcgcatatc gataccgtgg ttaacggcgg taacctcgac gggcaattcg
    10621 gcgcgctggc ggcgtggctg gcaattgact ggttgaaaac gcaatacggc gcaccgttac
    10681 gtacggtcga agtggtggcg atggcagaag aagaaggcag ccgcttcccg tatgtcttct
    10741 ggggcagtaa aaatatcttt gggctggcga atcctgacga cgtgcggaat atctgtgatg
    10801 ccaaaggaaa tagttttgcc gatgcgatga aggcttgcgg atttactctg ccgaacgccc
    10861 cactaactcc gcgtcaggct ataaaagcct ttgttgaact gcatattgaa cagggctgtg
    10921 tgctggaaag taatgggcaa tcaattggcg tggtgaatgc aattgtcggg cagcgtcgct
    10981 atacggtgac gctgaacggc gaatcaaacc atgcaggcac cacgccgatg ggttatcgtc
    11041 gtgatacggt ttacgctttc agtcgcattt gccatcagtc gatcgaaaaa gcgaaaaaga
    11101 tgggcgatcc gctggttctg acctttggca aagtagagcc gcgcccgaat acggtaaatg
    11161 tggtgccggg taaaaccacg ttcaccattg attgtcgtca taccgacgct gccgtgctgc
    11221 gcgatttcac ccaacagtta gaaaacgaca tgcgggcgat ttgcgatgaa atggacattg
    11281 gtattgatat cgatttatgg atggacgaag aacccgtgcc gatgaataag gagctggtcg
    11341 ccaccctgac agaattgtgt gaaagagaaa aactgaatta ccgggtgatg cacagtggtg
    11401 ccgggcacga cgcgcaaatt ttcgcgcctc gcgtaccaac ctgcatgatt tttatcccca
    11461 gcatcaacgg gatcagccat aacccggcgg aacgcaccaa tattactgac cttgccgaag
    11521 gggtcaaaac gttggcactc atgctttatc aacttgcctg gcaggaataa ggagtcataa
    11581 atgggatatt taaataacgt caccggttac cgcgaagatt tactggctaa ccgtgcgatt
    11641 gttaaacacg gtaatttcgc actgttaacc ccggatggtc tgataaaaaa tattattccg
    11701 gactttgaaa attgtgacgc gacaatcctc tccacgccaa agctgggtgc ctcttttgtt
    11761 gattatctgg tcacactgca tcaaaacggt ggcaaccaac agggcttcgg tggcgaaggc
    11821 attgaaacgt tcctgtatgt gatctctgga aatatcactg ccaaagccga aggcaaaaca
    11881 tttgccttaa gcgaaggtgg ctatctttat tgcccaccag gctccttaat gacgtttgtt
    11941 aacgcccagg ccgaagacag ccaaatcttt ttatataagc gccgctatgt tccggtagaa
    12001 ggccatgcac cgtggctgtt ttctggcaat gccagcgaac tggaacgcat tcactatgaa
    12061 ggtatggatg atgtgatcct gctggatttt ctgcccaaag agttaggttt tgatatgaac
    12121 atgcatatcc tctcttttgc accaggtgcc agccacggtt atatcgaaac acacgttcag
    12181 gaacatggtg cctatattct ttccggtcag ggggtttata
//
LOCUS       DAEBXN010000117        12158 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.117, whole genome shotgun sequence.
ACCESSION   DAEBXN010000117 DAEBXN010000000
VERSION     DAEBXN010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12158
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.117"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            130..999
                     /locus_tag="KJL99_003425"
     CDS             130..999
                     /locus_tag="KJL99_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HBD4438023.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1202..1555)
                     /locus_tag="KJL99_003426"
     CDS             complement(1202..1555)
                     /locus_tag="KJL99_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HBD4438024.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1693..3339)
                     /gene="groL"
                     /locus_tag="KJL99_003427"
     CDS             complement(1693..3339)
                     /gene="groL"
                     /locus_tag="KJL99_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HBD4438025.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3383..3676)
                     /locus_tag="KJL99_003428"
     CDS             complement(3383..3676)
                     /locus_tag="KJL99_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HBD4438026.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3952..5208
                     /gene="yjeH"
                     /locus_tag="KJL99_003429"
     CDS             3952..5208
                     /gene="yjeH"
                     /locus_tag="KJL99_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HBD4438027.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTLGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLVGLWLFLPKRKTPENGITT"
     gene            complement(5224..5700)
                     /gene="fxsA"
                     /locus_tag="KJL99_003430"
     CDS             complement(5224..5700)
                     /gene="fxsA"
                     /locus_tag="KJL99_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HBD4438028.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            6037..7473
                     /gene="aspA"
                     /locus_tag="KJL99_003431"
     CDS             6037..7473
                     /gene="aspA"
                     /locus_tag="KJL99_003431"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HBD4438029.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7591..8892
                     /gene="dcuA"
                     /locus_tag="KJL99_003432"
     CDS             7591..8892
                     /gene="dcuA"
                     /locus_tag="KJL99_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HBD4438030.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            9008..9346
                     /gene="cutA"
                     /locus_tag="KJL99_003433"
     CDS             9008..9346
                     /gene="cutA"
                     /locus_tag="KJL99_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HBD4438031.1"
                     /translation="MLDEKSSNTTSVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9322..11019
                     /gene="dsbD"
                     /locus_tag="KJL99_003434"
     CDS             9322..11019
                     /gene="dsbD"
                     /locus_tag="KJL99_003434"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HBD4438032.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADEQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLDIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPSGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWPGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            11056..11631
                     /locus_tag="KJL99_003435"
     CDS             11056..11631
                     /locus_tag="KJL99_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBD4438033.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11738..11813
                     /locus_tag="KJL99_003436"
     tRNA            11738..11813
                     /locus_tag="KJL99_003436"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11771..11773,aa:Phe,seq:gaa)
BASE COUNT         2933 a         3140 c         2980 g         3105 t
ORIGIN      
        1 tcttttctgt ttatatagag tgagcacggc tatttatgtt attttaacaa ctattactac
       61 caagcccatt tatctggctt ataaattaaa attaattatt taattgagaa atggttaggg
      121 agaacctaca tggccataag catcaagggt gttaataccg gtgttattcg caagagtaat
      181 aatttcattg cactggcgct gaaaattaaa gaacctcgca ataaagaatc gttgttcttt
      241 atgtctgtca tggaactgcg cgacctgctg attgcgctgg aaagtcgtct gcatcaaaag
      301 cataaactgg atgctgctgc acgccttcag tatgagcaag ctcgcgataa agtcattaag
      361 aaaatggcag aaaacatccc agaaattctg gttgatgagc ttaaaaatgc cgatattaat
      421 cgtcgggtta acacgctgga acttaccgat aatcaaggcg aaaatcttac cttcgtatta
      481 acgctacatg acggtagcaa atgtgagtta gtcgtcaatg agttgcagat tgaaatgctg
      541 gcgcgagcaa tcattcatgc aattaataat gcggaaatgc gtgaactggc attacgtatt
      601 acctcactgt tagatttcct gccgttatac gacgttgact gccaggacaa tggcaatctg
      661 gaatatgaca cctattccca accggaatgg aaacataact tgttcgacca ttatctggcg
      721 gtgctttacc gctttaaaga tgaaagtggc aaagaacaat ttagcggcgc agtggtgaaa
      781 acgcgtgaag caacgccggg taaagaaata gaggcgatca ctcgccgaat gcttgatttc
      841 agcccacgac tgaaaaaact cgcgggtgtg ccatgtcagg tctatgttcg caccgtagcg
      901 gcgaataacg cccaaccgct aacccaggat caatgcttac gcgcgctgca tcacttacgt
      961 gtacaatcca ccagcaaaac tgcaccacag gcgaaataat ctcatctttg ctaatgcgag
     1021 gctggagcca tccagcctga aatgactgat aacgtgatcg ttggtcatgc cggatgcggc
     1081 gtaaacgcct tatccggcct acaaattctc aagcattcaa tcagttgcaa tgcctgctgt
     1141 aggcctgata agcgaagcgc atcaggcaat aatacgtttg tcacccagcc tctggatcac
     1201 atcagttcgg gcacttataa acctgaccga taatctggct atccgtcggg acaaaactgg
     1261 acaacattcc ctgcgacggg ctgctgatgc cataaatcac gttaccgccc attgcagccg
     1321 cctggttgcg cagatcgttt gctgcgccgc gcatagaacc gccctcttct ccgtgttgcc
     1381 cggaaagcca gttgctttgc ttacctgtcg cagtaccaat cagctggcac tctgcgcctg
     1441 gctgctcgtc cacaatgcgt acactctgac cggcagcact caattcgttg ctggagctac
     1501 aacccgccat cagtagcgcg gcaccgacaa tccctgctaa gtattttacg tgcatgttat
     1561 tccccataat caatgagctg gacgccactc gcgtcatccg tgtctgaatc ttatactaga
     1621 aagatgacca aaagaaaaac ccccagacat ttctgcccgg gggtttgttt atttctgcga
     1681 agtgcagggc aattacatca tgccgcccat gccacccatg ccgcccatac cgccagcagc
     1741 gcctaagtca gctgcatcgt ttttcggcag gtcggtaacc atgcattcgg tggtgatcat
     1801 caggccagcc acagaagctg cgtactgcag agcagaacgg gttactttgg ttgggtccag
     1861 gatacccatg tcgatcatgt tgccgtattc ttcggttgct gcgttgtaac cgtagttgcc
     1921 gtcgccgcct ttaacggtgt tagcaacaac agacggttct tcgccgcagt tcaggacgat
     1981 ctgacgcagc ggagcttcca ttgcacgcag tgcaactttg atacccacgt tctggtcttc
     2041 gttctgacca cgcaggtcag ccagtttaga cgctacgcgg atcagcgcaa caccaccacc
     2101 agcaaccacg ccttcttcta ccgcagcacg ggtcgcgtgc agggcatctt caacgcgtgc
     2161 ttttttctct ttcatttcaa cttcggtagc agcacctact ttgataactg caacgccgcc
     2221 tgccagtttc gctacgcgct cctgcagttt ttcacggtcg tagtcagaag ttgcttcttc
     2281 aatctgctga cggatctgag caacacggcc ctggattgca gcttcttcac ccacgccatc
     2341 gatgatagtg gtggtgtctt tgttgatcac aacacgttta gcctgaccca ggtcttccag
     2401 ggttgctttt tccagctcca taccgatctc ttcagagatt acggtaccgc cagtcagggt
     2461 tgcgatatcc tgcagcatag ctttacgacg atcgccgaag cccggagctt taaccgcagc
     2521 aactttcacg atgccacgca tggtgttaac aaccagagtt gctagcgctt cgccttcaac
     2581 atcttcagcg atgatcagca gcggtttgcc tgctttggca acagcttcca gaaccggcag
     2641 catttcgcgg atgttggaga ttttcttgtc agccagcagg atgaacgggc tttccagttc
     2701 tactgcgcca gtttccggct tgttgatgaa gtaaggagac aggtagccac ggtcgaactg
     2761 cataccttca accacgtcca gttcgtcctg cagaccggta ccgtcttcaa cggtgataac
     2821 gccttcttta ccgactttgt ccatcgcttc agcgatcagt ttacctacgg tttcgtcgga
     2881 gttagcggag atggtaccaa cctgagcaat cgctttagaa tcagagcacg gtacggacag
     2941 cgctttcagt tcttcaactg cagcggtaac cgctttgtcg ataccacgtt tcaggtccat
     3001 cgggttcatg cccgcagcaa cagctttcag accttcagtg atgatagcct gagccagtac
     3061 ggttgcagtg gtggtaccgt cgcctgcagc gtcgttcgct ttggaggcaa cttctttcac
     3121 catctgcgca cccatatttt cgaacttgtc ttccagttcg atttcacgag caacggaaac
     3181 accatctttg gtgatggtcg gtgcaccgaa agatttatcc agaactacgt tacggccttt
     3241 tggaccgagg gtaactttca ctgcatctgc cagtacgttt acgccgcgca gcattttcac
     3301 acgagcgtcg ttaccgaatt ttacgtcttt agctgccatt atctttattc cttaaattcg
     3361 tatgttcagt gtcgtgcgcg gattacgctt caacaattgc cagaatgtcg ctttcggaca
     3421 tgatcaacac ttcttcattg tcgatcttct cggatttcac accgtagcca tcgttgaaaa
     3481 taacgatgtc gccaactttc acgtccagcg gcttcacttc gccattttca aggatacggc
     3541 cattgccgac agccagcact tcgccgcggg tggatttagc cgctgcagag ccggtcagaa
     3601 cgatgccgcc agcagattta gtttcaactt ctttacgctt gacgatcacg cgatcatgca
     3661 atggacgaat attcattgat aactctcctt tgagaaagtc cgtatctgtt atgggtgacg
     3721 ccggagctta cgtggtttcc cggctggtga ccagagaaat ggggatgagg cttcgcccct
     3781 tcaaggggga aaaagaaaaa aaattctgat cagcacaaaa tcgggtgaaa accctgattc
     3841 acctcacatt tcatcgcaat ttcttcatcg ccgtcataag cgaatctgat tgtgctacca
     3901 tcgaaaatct acgcagttgc ccaaaatttg ggcgcaatcg gatatcaacc catgagtgga
     3961 ctcaaacaag aactggggct ggcccagggc attggcctgc tatcgacgtc attattaggc
     4021 actggcgtgt ttgccgttcc tgcgttagct gcactggtag caggcaataa cagcctgtgg
     4081 gcgtggcccg ttttgattat cttagtgttc ccgattgcga ttgtgtttgc gattctgggt
     4141 cgccactatc ccagcgcagg cggcgtcgcg cacttcgtcg gtatggcgtt tggttcgcgg
     4201 cttgagcgag tcaccggctg gctgttttta tcggtcattc ccgtgggttt gcctgccgca
     4261 ctacaaattg ccgccggatt cggccaagca atgtttggct ggcatagctg gcaactgttg
     4321 ttggcagaac tcggtacgct ggcgttggtg tggtatatcg gtactctcgg tgccagttcc
     4381 agtgctaatc tacaaaccgt tattgccgga cttatcgtcg cactgattgt cgctatctgg
     4441 tgggcgggcg atatcaaacc tgcgagtatc cccttccccg cgccaggaaa tatcgaactt
     4501 accgggttat ttgctgcgtt atcagtgatg ttctggtgtt ttgtcggtct ggaagcattt
     4561 gcccatcttg cctcggaatt taaaaatcca gagcgtgatt ttcctcgtgc tttgatgatt
     4621 ggtctgctgc tggcaggatt agtctactgg ggctgtacgg tagtcgtctt acacttcgac
     4681 gcctatggtg aacaaatggc ggcggcagca tcgcttccaa aaattgtagt acagttgttc
     4741 ggtgtaggag cgttatggat tgcctgcgta attggctatc tggcctgctt tgccagtctt
     4801 aacatttata tacagagctt cgcccgcctg gtctggtcgc aggcgcaaca taatcctgac
     4861 cactacctgg cacgcctctc ttctcgccat atcccgaata atgccctcaa tgcggtgctt
     4921 ggctgctgcg tggtgagcac gttggtaatt catgccttag agatcaatct ggacgctctt
     4981 attatttatg ccaatggcat ctttattatg atttatctgt tatgcatgct ggcaggctgt
     5041 aaattattgc aaggtcgtta tcgactactg gcggtggttg gcgggctgtt atgcgttctg
     5101 ttactggcaa tggtcggctg gaaaagtctc tatgcgctga tcatgctggt ggggttatgg
     5161 ctgtttctgc caaaacgaaa aacgccggaa aatggcataa ccacataatc cggcgtttcg
     5221 acattaatcc tggcgatcgt ctttatgatc aaggcggtcg cggtcatcat cctttcgctg
     5281 atactcacca tcaaaagtat taccgccacc ggtcccggcg ctaaaaccgc cgcctggcat
     5341 gcgagaaaag cgcaaatgtg gcatcaactt cactgtcaga tgcttttgca ccggcggcaa
     5401 taaaagcaga agaccgagga agtcggtgaa aaagcccggt aataaaagca gcaaaccggc
     5461 aataatcagc gaaacacttt taatcatctc cgccgcaggg ttttcacccg ccgccatttt
     5521 ttgctgcatc agcacgaaat tcttaaagcc ctggttacgt accagtgaca taccgataac
     5581 tgacgtgaat ataaccagca cgagggtcag caataccccc aatacatggg caacctgaat
     5641 aaagattgaa atctcaatat agacataaag gaaaatggca ataaaaggta accagcgcaa
     5701 aggtttctcc tgtaatagca gccggttaaa cccggctacc tgaatgggtt gcgaatcgcc
     5761 ttcagcttat attgtggtca ttagcaaaat ttcaagatgt ttgcgcaact atttttggta
     5821 gtaatcccaa agcggtgatc tatttcacaa attaataatt aaggggtaaa aaccgacact
     5881 taaagtgatc cagattacgg tagaaatcct caagcagcat atgatctcgg gtattcggtc
     5941 gatgcagggg ataatcgtcg gtcgaaaaac attcgaaacc acatatattc tgtgtgttta
     6001 aagcaaatca ttggcagctt gaaaaagaag gttcacatgt caaacaacat tcgtatcgaa
     6061 gaagatctgt tgggtaccag ggaagttcca gctgatgcct actatggtgt tcacactctg
     6121 agagcgattg aaaacttcta tatcagcaac aacaaaatca gtgatattcc tgaatttgtt
     6181 cgcggtatgg taatggttaa aaaagccgca gctatggcaa acaaagagct gcaaaccatt
     6241 cctaaaagtg tagcgaatgc catcattgcc gcatgtgatg aagtcctgaa caacggaaaa
     6301 tgcatggatc agttcccggt agacgtctac cagggcggcg caggtacttc cgtaaacatg
     6361 aacaccaacg aagtgctggc caatatcggt ctggaactga tgggtcacca gaaaggtgaa
     6421 tatcagtacc tgaacccgaa cgaccatgtt aacaaatgtc agtccactaa cgacgcctac
     6481 ccgaccggtt tccgtatcgc agtttactct tccctgatta agctggtaga tgcgattaac
     6541 caactgcgtg aaggctttga acgtaaagct gtcgaattcc aggacatcct gaaaatgggt
     6601 cgtacccagc tgcaggacgc agtaccgatg accctcggtc aggaattccg cgctttcagc
     6661 atcctgctga aagaagaagt gaaaaacatc caacgtaccg ctgaactgct gctggaagtt
     6721 aaccttggcg caacagcaat cggtactggt ctgaacacgc cgaaagagta ctctccgctg
     6781 gcagtgaaaa aactggctga agtcactggc ttcccatgcg taccggctga agacctgatc
     6841 gaagcgacct ctgactgcgg cgcttatgtt atggttcacg gcgcgctgaa acgcctggct
     6901 gtgaagatgt ccaaaatctg taacgacctg cgcttgctct cttctggtcc acgtgccggc
     6961 ctgaacgaga tcaacctgcc ggaactgcag gcgggctctt ccatcatgcc agctaaagta
     7021 aacccggttg ttccggaagt ggttaaccag gtatgcttca aagtcatcgg taacgacacc
     7081 actgttacca tggcagcaga agcaggtcag ctgcagttga acgttatgga accggtcatt
     7141 ggccaggcca tgttcgaatc cgttcacatt ctgaccaacg cttgctacaa cctgctggaa
     7201 aaatgcatta acggcatcac tgctaacaaa gaagtgtgcg aaggttacgt ttacaactct
     7261 atcggtatcg ttacttacct gaacccgttc atcggtcacc acaacggtga catcgtgggt
     7321 aaaatctgtg ccgaaaccgg taagagtgta cgtgaagtcg ttctggaacg cggtctgttg
     7381 actgaagcgg aacttgacga tattttctcc gtacagaatc tgatgcaccc ggcttacaaa
     7441 gcaaaacgct atactgatga aagcgaacag taatcgtaca gggtagtaca aataaaaaag
     7501 gcacgtcaga tgacgtgcct tttttcttgt gagcagtaac ttaaaaataa caatctaata
     7561 tcaacttgtt aaaaaacaag gaaggctaat atgctagttg tagagctcat catagttttg
     7621 ctggcgatct tcttgggcgc cagattgggg ggaataggta ttggttttgc aggcggattg
     7681 ggggtgctgg ttcttgccgc tattggcgtt aaacccggta acatcccgtt cgatgtcatt
     7741 tccattatca tggcggttat cgccgctatt tctgccatgc aggttgctgg cggtctggac
     7801 tatctggttc atcagacaga aaagctgctg cgccgtaacc cgaaatacat cacgatcctc
     7861 gcaccgatcg tgacctattt cctgactatc tttgctggta ctggcaacat ctctctggcg
     7921 acactgccag ttatcgctga agttgcgaag gaacaaggcg ttaaaccttg ccgtccgctc
     7981 tctactgcag tggtatccgc gcagattgcg atcaccgcat cgccaatctc agcggcagtg
     8041 gtttacatgt cttccgtgat ggaaggtcat ggcatcagct acctccatct gctctccgtg
     8101 gtcatcccgt ccaccctgct ggcggttctg gtgatgtcct tcctggtcac tatgctgttc
     8161 aactccaaac tctctgacga tccgatttat cgcaagcgtc tggaagaggg cttggttgaa
     8221 ctgcgcggtg aaaagcagat tgaaatcaaa tccggtgcaa aaacgtccgt ctggctgttc
     8281 ctgctgggcg tagttggcgt ggttatctat gcaatcatca acagcccaag catgggtctg
     8341 gttgaaaaac cgctgatgaa caccaccaac gcaatcctga tcatcatgct cagcgttgca
     8401 actctgacca ccgttatctg taaagtcgat accgacaaca tcctcaactc cagcaccttc
     8461 aaagcaggta tgagcgcctg tatttgtatc ctgggtgttg cgtggctggg cgatactttc
     8521 gtttccaaca acatcgactg gatcaaagat accgctggtg aagtgattca gggtcatccg
     8581 tggctgctgg ccgtcatctt cttctttgct tctgctctgc tgtactctca ggctgcaacc
     8641 gcaaaagcac tgatgccgat ggctctggca ctgaacgttt caccgctgac cgctgttgct
     8701 tctttcgctg cggtgtctgg tctgttcatt ctgccgacct acccgacgct ggttgctgcg
     8761 gtacagatgg atgacacggg tactacccgt atcggtaaat tcgtcttcaa ccatccgttc
     8821 ttcatcccgg gtactctggg tgttgccctg gccgtttgct tcggcttcgt gctgggtagc
     8881 ttcatgctgt aatgatcaat cgcggggcgt tcacgccccg ctttctttcc cgccgactaa
     8941 catccttccc ccgtccgttg tatagtgacc tctctcttgc ggttccatct gttcttgcga
     9001 ggtgtttatg cttgatgaaa aaagttcgaa taccacgtct gtcgtggtgc tatgtaccgc
     9061 accggatgaa gcgacagccc aggatttagc cgccaaagtg ctggcggaaa aactggcggc
     9121 ctgcgcgacc ttgatccccg gcgctacctc tctctattac tgggaaggta agctggagca
     9181 agaatacgaa gtgcagatga ttttaaaaac taccgtatct caccagcagg cactgctgga
     9241 atgcctgaag tctcatcatc catatcaaac cccggaactt ctggttttac ctgttacaca
     9301 cggagacaca gattacctct catggctcaa cgcatcttta cgctgattct gctactttgc
     9361 agcacttccg tttttgccgg attattcgac gcgccgggac gttcacaatt tgtccccgcg
     9421 gatcaagcct ttgcttttga ttttcagcaa aaccaacatg accttaatct gacctggcag
     9481 atcaaagacg gttactacct ctaccgtaaa cagatccgca ttacgccgga acacgcgaaa
     9541 attgccgacg agcagctgcc gcaaggcgtc tggcatgaag atgagtttta cggcaaaagc
     9601 gagatttacc gcgatcggct gacgcttccc gtaaccatca accaggcgag tgcgggagcg
     9661 acgttaactg tcacctacca gggctgtgct gatgccggtt tctgttatcc gccagaaacc
     9721 aaaaccgttc cgttaagcga agtggtcgcc aacaacgcag cgtcacagcc tgtgtctgtt
     9781 tcgcagcaag agcagcacac cgcgcaattg cccttttccg cgctctgggc gttgttgatc
     9841 ggtattggta tcgcctttac gccatgcgtg ctgccaatgt acccactgat ttctggcatc
     9901 gtgctgggtg gtaaacagcg gctctccact gccagagcat tgttgctgac ctttatttat
     9961 gtgcagggga tggcactgac ttacacggcg ctgggtctgg tagttgccgc cgcagggtta
    10021 cagttccagg cggcgctaca gcacccatac gtgctcattg gcctcgacat cgtctttacc
    10081 ttgctggcga tgtcaatgtt tggcttgttt actctacaac tcccctcttc gctgcaaaca
    10141 cgtctcacgt tgatgagcaa tcgccaacag ggcggctcac ctagcggtgt gtttgttatg
    10201 ggggcgattg ccggactgat ctgttcacca tgcaccaccg caccgcttag cgcgattctg
    10261 ctgtatatcg cccaaagcgg gaacatgtgg ccgggcggcg gcacgcttta tctctatgcg
    10321 ttgggcatgg gcctgccgct gatgctaatt accgtctttg gtaaccgctt gctgccgaaa
    10381 agcggcccgt ggatggaaca agtcaaaacc gcgtttggtt ttgtgatcct cgcactgccg
    10441 gtcttcctgc tggagcgagt gattggtgat gtatggggat tacgcttgtg gtcggcgctg
    10501 ggtgtcgcat tctttggctg ggcctttatc accagcctac aggctaaacg cggctggatg
    10561 cgcgtggtgc aaataatcct gctggcagcg gcattggtta gcgtgcgccc acttcaggat
    10621 tgggcatttg gtgcgacgca taccgcgcaa actcaaacgc atctcaactt tacacaaatc
    10681 aaaacggtag atgagttaaa tcaggcgctc gttgaagcca aaggcaaacc ggtgatgtta
    10741 gatctttatg ccgactggtg cgtcgcctgt aaagagtttg agaaatacac cttcagcgac
    10801 ccgcaggtgc aaaaagcgtt agcagacacg gtcttacttc aggccaacgt cacggccaac
    10861 gacgcacaag atgtggcgct gttaaagcat cttaatgtcc ttggcctacc gacaattctc
    10921 ttttttgacg gacaaggcca ggagcatcca caagcacgcg tcacgggctt tatggatgct
    10981 gaaaccttca gcgcacattt gcgcgatcgc caaccgtgaa cgacacttgc agtgggaaag
    11041 acggaggaaa atagcgtgca acgtgaagat gtactgggag aagccctgaa attattagaa
    11101 ttacaaggga ttgccaacac cacgctggag atggttgctg aacgtgtgga ttatccactg
    11161 gacgagctac gccgcttctg gccagacaaa gaggcaatcc tctacgatgc gctgcgttat
    11221 cttagccaac agatagatgt ctggcgtcgt cagctgatgc tggacgaaac gcaaaccgcc
    11281 gaacaaaagc tgctggcacg ttatcaggcg ttatcggagt gcgttaaaaa caaccgctat
    11341 ccgggctgtc tatttatcgc tgcctgtacg ttttatcccg atcctggcca ccctattcat
    11401 caactggccg atcagcaaaa aagcgctgcc tacgatttca cccacgaact gttaaccacg
    11461 ctggaagttg acgatccggc gatggtagca aagcagatgg aactggtgct ggaaggctgt
    11521 ttaagccgaa tgctggtgaa tcgtagccag gcggatgtcg acactgcaca tcggctggcg
    11581 gaagatatcc tgcgcttcgc ccgctgccgc cagggtggtg cattgacctg acagaaacac
    11641 agaaaagaag cgatttgccg caatcttaag cagttgaatc gcttttactg aaattaggtt
    11701 gacgagatgt gcagattacg gtttaatgcg ccccgttgcc cggatagctc agtcggtaga
    11761 gcaggggatt gaaaatcccc gtgtccttgg ttcgattccg agtccgggca ccaaattcat
    11821 atcaacggac ctccacggag gtccgttttt cgtttcagga cgccacgatt taagcgttct
    11881 gcctccaaat caattctacc gaactcaacc agattctccc cacatcacca gcaatttgcg
    11941 agcatatccc aatttgggaa aatttgttcc tgagctatag cactaactga cgtgaaatgt
    12001 cgtgcggccc cgtgatgctg ttgaacgtca aatgacgtca tcaggagcgt aacgtatcca
    12061 taaagcacaa caccggtctg aacgccgact gatgagattt tctgaatgag aacaaagaga
    12121 aatgtgtcag tccgcttgct catgcaaaga ctaacaat
//
LOCUS       DAEBXN010000118        12113 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.118, whole genome shotgun sequence.
ACCESSION   DAEBXN010000118 DAEBXN010000000
VERSION     DAEBXN010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12113
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.118"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..370
                     /locus_tag="KJL99_003437"
     CDS             <1..370
                     /locus_tag="KJL99_003437"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438034.1"
                     /translation="TTLMSWDAVLYDLRSEKIISKDNYLTDLNRRHLELVLHTNPNPS
                     GSVTKILRTIFTKQVKSLGPELCHFLHHELTKKRYEVWHHYDVLHYQSSSFSRSQFNA
                     LINLLAQQSRFADLKLSNYK"
     gene            complement(458..1102)
                     /gene="cheZ"
                     /locus_tag="KJL99_003438"
     CDS             complement(458..1102)
                     /gene="cheZ"
                     /locus_tag="KJL99_003438"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HBD4438035.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTHMLRDSLRELGLVQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(1113..1502)
                     /gene="cheY"
                     /locus_tag="KJL99_003439"
     CDS             complement(1113..1502)
                     /gene="cheY"
                     /locus_tag="KJL99_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HBD4438036.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(1517..2566)
                     /gene="cheB"
                     /locus_tag="KJL99_003440"
     CDS             complement(1517..2566)
                     /gene="cheB"
                     /locus_tag="KJL99_003440"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HBD4438037.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLATISAGQAIRI"
     gene            complement(2569..3429)
                     /gene="cheR"
                     /locus_tag="KJL99_003441"
     CDS             complement(2569..3429)
                     /gene="cheR"
                     /locus_tag="KJL99_003441"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HBD4438038.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEDRVWSAAASTGEEPYSIAMTLADTLGTVPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(3448..5049)
                     /gene="tap"
                     /locus_tag="KJL99_003442"
     CDS             complement(3448..5049)
                     /gene="tap"
                     /locus_tag="KJL99_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HBD4438039.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNHGE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSHEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNSSLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(5095..6756)
                     /gene="tar"
                     /locus_tag="KJL99_003443"
     CDS             complement(5095..6756)
                     /gene="tar"
                     /locus_tag="KJL99_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HBD4438040.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNFDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHICEIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSALAAKEIKALIEDSVSRIDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRITEQDPNWETF"
     gene            complement(6901..7404)
                     /gene="cheW"
                     /locus_tag="KJL99_003444"
     CDS             complement(6901..7404)
                     /gene="cheW"
                     /locus_tag="KJL99_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HBD4438041.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(7425..9389)
                     /gene="cheA"
                     /locus_tag="KJL99_003445"
     CDS             complement(7425..9389)
                     /gene="cheA"
                     /locus_tag="KJL99_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HBD4438042.1"
                     /translation="MSIDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASYDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKAGEVDLLEEELGHLTTLNDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKIATPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(9394..10320)
                     /gene="motB"
                     /locus_tag="KJL99_003446"
     CDS             complement(9394..10320)
                     /gene="motB"
                     /locus_tag="KJL99_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HBD4438043.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRLHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(10317..11204)
                     /gene="motA"
                     /locus_tag="KJL99_003447"
     CDS             complement(10317..11204)
                     /gene="motA"
                     /locus_tag="KJL99_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HBD4438044.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(11331..11909)
                     /gene="flhC"
                     /locus_tag="KJL99_003448"
     CDS             complement(11331..11909)
                     /gene="flhC"
                     /locus_tag="KJL99_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HBD4438045.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(11912..>12113)
                     /locus_tag="KJL99_003449"
     CDS             complement(11912..>12113)
                     /locus_tag="KJL99_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HBD4438046.1"
                     /translation="LPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGI
                     MLSTRLLNDVNQPEEALRKKRA"
BASE COUNT         2783 a         3388 c         2934 g         3008 t
ORIGIN      
        1 cactacgcta atgagttggg atgccgtgct ctatgatcta cgtagtgaaa aaattatcag
       61 taaagataat tacttgaccg acttaaaccg cagacatctt gaacttgttc ttcatactaa
      121 ccccaatcca agcggttcgg taacgaagat tttacgcact atttttacta agcaggtaaa
      181 gagtttaggg ccggaactct gtcatttctt gcaccatgaa ctgacgaaaa aacgttatga
      241 agtatggcat cactacgatg tactgcacta ccagtcatcg tcattttctc gttcacaatt
      301 taatgcattg ataaatttac tggcgcaaca atcacgcttc gctgatctaa aattatcaaa
      361 ctacaaataa ccatcctcca cgtactccct gaacgatacc cgccaccctg ctatcggctt
      421 ggcatcatcg cgcaggccga agttcaggca atacaaatca aaatccaaga ctatccaaca
      481 aatcgtccac ctgatcctga ctggctacca cgccggcttt gctggtatcg acctgaggtc
      541 cattaagcaa actctggttt tcacgttttg gacgcgactc ctgttccggg atgttttcca
      601 acagcaccat cagcaactgg cgttcgatct cctgaatgac atccatcatc cgcttaataa
      661 cctgcccggt gagatcctga aaatcctgcg ccatcatgat ttccagcagt tgcgcgttag
      721 taaagctggt atgcgcgggt acatctgcca gaaattgtcg tgtatctgtt accagttcac
      781 gggcgtcggc aaggtcaatc ggatcggcaa accagtcatc ccaacgttgg gttaacgctt
      841 ttgctgattt ctccatttga tcctgatgcg gttgtgacgc ctcaacactg ttcagcgccc
      901 gctccgcagc ctgggcggtc atctgcacaa catagtacaa acgatcgcgc gcatcgggga
      961 tggcttccgc cgcttcggca atggcctgaa ccagccccag ttcccgcaaa ctgtcgcgca
     1021 gcatatgcgt caggctgccg atgcgcgcaa tgatatcgcc agctgaatgc tcgtcagcag
     1081 gtttgattga tggttgcatc atagtcgcat cctcacatgc ccagtttctc aaagattttg
     1141 ttgagttttt cctccagcgt cgcggcggta aatggcttca ccacatagcc actggccccc
     1201 gcttgcgccg cagcaatgat gttctctttc ttcgcttctg cagtcaccat taacactggc
     1261 aatgccgaca tcgcgccatc cgcacgaatt gttttcagca attccaggcc atccatattg
     1321 ggcatgttcc agtcggagat aacaaatcca taaccgcctg cctgcaactt attgagagcg
     1381 tcgacaccat cttccgcttc ctcaacatta ttgaatccca gctctttcag caggttacgc
     1441 actatgcgtc gcatggtgga aaagtcatcc acaaccaaaa atttaagttc tttatccgcc
     1501 atttcacact cctgatttaa atacgtatcg cctgtccggc actaattgtt gccaacattt
     1561 gctggcttac ctggctaaga tcgaccactt cgcagacacc acccatattg atggcctcgc
     1621 gcggcatgcc gaacaccacg caacttgctt cgttttgcgc aagggtccat gcccccgcct
     1681 gacgcatcgc caacattccc gccgcgccgt cgttgcccat accggtcagg atcaccccaa
     1741 ccgcattacg ccccgcctgt ttggcgacag aatggaacaa cacatctacc gaaggccgat
     1801 gacggttaac cgccgggccg tcgtgaattt tgatttgata atttgcgcca ctacgcgcca
     1861 gttccatatg ccgatcgccc ggcgcaatat aggcatgccc cggcaagaca cgttctccgt
     1921 cttcggcttc tttaaccccg atctggcaaa gcttattaag tctgtcggca aaagagcggg
     1981 tgaaaccggg cggcatatgt tgggtaatta acaatgccgg gctggaaagc ggcaacggtt
     2041 gcagtacgtg acgaattgcc tcagttccac ccgttgaagc accaatcgca atcagttttt
     2101 cagaactcaa caacggccct gccttcagcg ttgtcggtgc tgacaatggc ttatgtgctg
     2161 caaggctcgc ctttgctgcc gtacgcacct tttcagcaat catttcgcta tacgccagca
     2221 ttccttcgcg aatacccagt tgcggtttgg tgacaaaatc tattgccccc agctccagcg
     2281 cgcgcagcgt gacttctgat cctttgccgg tcaaggaaga aaccatcaca acgggcattg
     2341 gacgcaaacg cattaatttt tcgaggaaat ccagtccgtc catccgcggc atttcaacat
     2401 ccagcgtcag cacatcggga ttgaatttct taatcaagtc acgcgcaacc agcggatcag
     2461 gcgcggtcgc caccatttcc atgtcgctat ggctgttgat gatttctgtc atgatctggc
     2521 gcatcagtgc cgaatcatcg acagataaca ccctgatttt gctcatcgtt aatccttact
     2581 tagcgcatac accgtctgac cacgcagcgt gaagcggcgc tcgaggtggc taaagttttc
     2641 agagtgaccc gcaaacagca atccgtcggg tttaaggagc ggaacaaagc ggcgcaaaat
     2701 ctcctgctgg gtagtttgat cgaagtagat catgacgtta cgacagaaga tcgcatcaaa
     2761 cggccccggc acggtgtact gtttcgccag taaattcagc ggggcaaaat caacatagtt
     2821 cgccagctcc tgacgcacgc gtaccagccc ttcatgcggt cccgtccctc gcatgaaata
     2881 ccgttgcagt tgctgcggcg tcaggttttt caactcttca tggcgataga taccgcttct
     2941 ggctttttcc agcacttcgg tgtcgatatc actggcaaac actttccagc gtccgggcac
     3001 ggtgcccaat gtgtcagcca gagtcatcgc aatgctgtac ggctcttcgc cggtcgaagc
     3061 cgccgcgctc catacgcgat cctcgccaga acggcgacgt gcgtgatccg cgagcagagg
     3121 gaaatgatga gcctcacgga aaaatgccgt cagattcgtg gtcagcgaat tgataaacgc
     3181 ctgccactca ccgctgtgct gattagattc cagcaagttc agataatgac cgaaatccgt
     3241 cagtcccaga gaacgcaaac gacgaaccag tcggttgtaa accatgtcgc gtttatggtc
     3301 agccagaacg atcccggctc gttgatagat caattgactt atccgccgaa aatgcgcgtc
     3361 ggaaagcgcc aggcgctcgg tcatctgtaa caataaagac gtttgcccac agggcagaga
     3421 tgaagtcata gcgccttctc aatcacttca ggataccact ggcgcaattt gtaactgcgc
     3481 cgactcatgt cgtgctactt catgttcttc aagggtaaat accgccacac gcgacgaaag
     3541 atggtcggcc tgattcgcca gttgttccgt tgccaccgcc gcttcttcta ccagcgagga
     3601 gttctgctga gtcacctgat ccatctggct gacagcctgt gcaacctgct caatcccccg
     3661 ccgttgttct tccgacgccg aagcaatttc tcccataatg tcgttcacgc gggtcaccga
     3721 actgacaata tcggtcatgg tcgcggcggc gttattcacc agtttcgaac cctgctggac
     3781 acgattgact gactcttcga tcagcccttt gatctctttt gctgcctgcg cgctacggct
     3841 ggcaagattg cgcacttcac ctgccactac cgcaaaacca cgcccctgct ctccggcgcg
     3901 agccgcttcc actgccgcat tcagggccag aatattggtc tggaaagcaa ttccgtcgat
     3961 aacgctgata atgtcgccaa ttttttgcga gctggtggcg atctcctgca tggtgtgagt
     4021 catggtactg acctgaacac cgcccgcctg cgccgttgtc gcggcatttt ttgccagttc
     4081 cgacgcctgt cgtgcgttat cggcgttttg ccctaccgtg gcggttaatt gctccatact
     4141 ggcggccgtt tgtgccagcg atgccgcctg ctgttcggta cgacttgaga gatcgttatt
     4201 gcctgcgaca atctccgcga tcccaatgtg catctcatgg cttcccttac gcacatcact
     4261 caccgtccca cgcaaagcct gctgcatggt cttcaggctg gcaaaaatgg cggtgatctc
     4321 attacgacca tataccgcaa tcggacgcgc cagattaccc gcagcaatgc tgtcaaaatg
     4381 gctaccgata atggccagtg gttgaacaat catcttgcgc gtccaccaca gcgcactgct
     4441 gatgtagatc gccgcaacaa taatcatgct gataaacacc agcgccgaaa tctgatagtt
     4501 acgctggctt tgcgcactgg cggcttccag cacatggttg atctccagct gccaggcctc
     4561 aaagttaacg tcaaacgcat tctgtgatcc ctgcaccggc gcagtgagga aatccgaaag
     4621 ttgattactt tcaagccagg tcgcctgatg ttcgagatcg ttgtgccagc gggcaaaact
     4681 tttctccgtc tctttttgca atgccctgac gtgctcgttg cccgcagtca tcgccataaa
     4741 acttttaaac agcgtggtgg attgcgtcag actggcgcgc gccgtcgtca tcaacgtttt
     4801 aatgtcatcc gccggatagc taagcgctgt cagagtgcct gctttgttca gcgcggtact
     4861 ggcctgtaac attaccgccc gagtttgcgc taatgccgca cgttgctgat tgctctgctc
     4921 gccgtgattc agtcgttgca aatcgtcgcg aaatgcccaa aaagacatgc cgttactgcc
     4981 aatctgcaag atcccgcaga gaatcaaaat taaaaacagc gtggtcgaaa ttcgaatacg
     5041 attaaacatc aacgctcccc atcaggcggc aatgaccgcg ttagtaaaaa ctcgtcaaaa
     5101 tgtttcccag tttggatctt gttcagtaat tcgcagtcgt ggctgtgccg gtggttgctc
     5161 actggcagga cgggatggtg tttgcggttt attggtgagt gggctggctg ccagacggaa
     5221 cgcggaaacc gcttgcgtta aacgactcgc ctgttcttcc agcgcagctg cggcggcagc
     5281 tgattcctgc accagcgatg cgttctgttg cgtgacgcga tccatttccg aaaccgccaa
     5341 tgcgacttga tcgatgccac ggctctgttc atccgatgcc gatgcaatct cgcccataat
     5401 gtcagtcacg cgagtgacag cattgacgat attgttcatt gtttccccgg cgctttcgac
     5461 cagcaccgaa ccggtatcaa tgcgcgagac ggagtcttca atgagggctt tgatctcttt
     5521 tgccgccagg gcgctgcgac tggcaagatt acgcacttca cccgccacca cggcaaaacc
     5581 acggccctgt tcacccgcac gcgcggcttc aaccgcggca ttcagcgcga ggatattagt
     5641 ctggaaggca ataccgtcga taacgctgat aatgtcggca attttcttcg aactatcggc
     5701 gatctcatgc atcgttttca ctacgccatc caccactttg ccgccgtgct gggcggtgtc
     5761 ggaggcactt tgcgccagtt gcgaggcctg gcgggcgtta tcggcgtttt gcttcactgt
     5821 cgcggtgagc tgctccatgc tggcggcagt ttcttccagc gcggatgcct gctgttcagt
     5881 acgggaggaa agatcggtgt tgcccgccgc aatttcacgg gtaccggcat agatggcatc
     5941 tgaaccttcg cggacatgag tgacggtgtc agtcaaagag cgttgcatat gtgaaacgct
     6001 ctgcgccagg tcacccattt cactgcgccc gtcaatggtc agggtattcg ccaggttacc
     6061 accggcgatt tcgcaaatgt gagcaataat ttttgccagc ggagtaagca acatacggcg
     6121 aatgccgtac cacgccacca gcagaatcaa taccaccacc agcgcgataa ccgccagttg
     6181 ccactgggca aatcggtaat catctgcgtt gtcagtgacg atatcgcgat acagtttttc
     6241 actgctgagg gcgtactgag caaacgcttc gcccattgca ttttgcattc cctgggttgg
     6301 ctgagcgaaa taagctccag tattgccata atcaagataa tcaatcagtt cagttaacgc
     6361 tgtgtaatag tttttatatt tttcatcaaa attacggctg gtagcgacca tttcaggtaa
     6421 cggtgccatg cttttgaatt ttttataatg cgtcgcggcc tgggccaatg ttttcctggc
     6481 gctatcgagc aattcaactt tggcgttact ttgttgattg gaggaatcca tcatcatccg
     6541 caccgctgaa cgactcaggt taatgcgcgt ttgcagcatt aaatcccagg ttgacgtcag
     6601 ctcgccctgc tgttcccgta attgattgga aaccacaaag ctcttctggc tatggtgaag
     6661 ggaagaaaaa aacaaactgc cggaaataag ctgtaacagt gcgaataccc ccagcaccat
     6721 taccaacagc gtgactacgc ggatacggtt aatcataagg caccttcctg aaaacaagtt
     6781 gatctcgtta tcggcaagaa ggggggaaac tttattgctg atgccacccg ccgcgaaatt
     6841 gaaataaaaa acccgatgcg cagatcatcg ggttcatttc aattgaggaa atcgggagaa
     6901 ttacgccact tctgacgccg cgctatctaa cagcgccatc tcttcgctgt tcagcagttt
     6961 ttcgatgttc accagaatca acatacggtc gcccagtgcg cccagtccag tgagatattc
     7021 tgttgaaagc gtcacggcaa actctggtgc cggacgaatt tgctccgccg tcaatgaaag
     7081 cacgtctgaa acgccgtcaa ccacgatgcc gaccacccgc tgtccgagat tcaggacgat
     7141 aactaccgtg ttgtcgttat agtccacatc cacctggctg aacttaattc gtaagtcaac
     7201 aatcggcaca ataacgccgc gcagattcgt gacgcctttg ataaacgctg gcgtgttcgc
     7261 aatccgtgtt acctgatcgt agccacggat ctcctgcact ttcaggatat caataccgta
     7321 ctcttcatca ccaagggtaa ataccagaaa ttcctggcct gacggctcgc tggccagctt
     7381 tgttacattc gtcataccgg tcatattgtt acctttttac tcattcaggc ggcggtgttc
     7441 gccatacgtt gttcgcggtt tatcgcctgc aaggcggaga catcaacaat cagtgccacg
     7501 ctgccgtcgc caagaatggt cgcagcagaa atgccgggga ctttgcgata gttactttca
     7561 aggtttttaa ccacaacctg gtgttgacca attaattgat ccaccagcaa ggcgtagcgg
     7621 cgaccgccac tttgtaagat caccacaatt ccctgggtgg cttcggtttt cgcgcccgcg
     7681 acgttgaaca ctttccacag ttcgacgatg ggcagatatt caccccgcac ttccagcacc
     7741 cgctcgccgc cggccagtgg atggagatcg gcttcacggg gttgcagtga ttccataaca
     7801 gcattcagcg gcagaatgaa aacttcatcc gcaacgcgta cggacatgcc gtcgaggatg
     7861 gccagcgtca gcggcagtaa aatgcggatc gtagtgccag taccctgctt cgactggatt
     7921 tcgacatgac cgcccatctc ctggatatta cgtttaacga cgtccatgcc gacgccgcgc
     7981 ccggagacgt cggtgacctg ctctgccgtg gagaagcctg gtgcaaatat cagcattgca
     8041 acttcgtcgt cgctcatgtt ttcgctgaca gtcaaacctt gcgaggccgc ttttgccaga
     8101 attcgctcac ggtttagccc cgccccatcg tcggtcactt caatgcaaat gttgccgccc
     8161 tgatgttcgg cagacagaat taaatttccg acgctgtttt tacctgcggc gagccgtttt
     8221 tctggcagtt caataccgtg atcgaggcta ttgcgtacca ggtgggtcag cgggtcgata
     8281 atgcgttcta tcaggctctt gtcgagctcg gtggaactgc ccaccagcgt cagttctacc
     8341 tgcttgccga gttttcccgc cagatcacgc accagccggg gatagcgact aaagacatat
     8401 tccatcggca tcatgcgaat cgacatcact gattcctgca aatcacgggc gttacgttgt
     8461 aactgcccca tgctggtaat caaatcacca tgattaaccg ggtccagttc gctggaacgc
     8521 tgggcaagca tggactgggt gataaccagc tcgccgacga ggttaattaa ttgatcaacc
     8581 ttttctaccg ctacacggat gctggtggat tcactgctgc gcgtcgtttt ttcccgctcc
     8641 acgcggccgg ttggcgcttg ttcggctgcc agtttaagca caggtggggt agctattttt
     8701 ggcgaaactt ctactgtttc aaaggtaatc tgatcggctt caatcacaaa acagagtacc
     8761 gctgtgatgt catcttcggc gatatcgccc ggtaatattg ccgagagaga atccgcccct
     8821 ttcaccacgt catttaacgt tgtcagatgc cccagctctt cttccagcag gtcgacttcg
     8881 ccggccttca ggcgcgaaag gataattcgt cgcggcaact gactgcgact ttgctcatct
     8941 tgcggttcac ttttggcaac cacacttaat cgggtcactg cggatggcgt ttcgcctttc
     9001 gcttctaatg ccagttgacg caaggcctgg cagatataat cgtagctggc ggcatccggc
     9061 tcttgcgact gtttataagc gtcgagctgt tcttgcatga tgtccttcgt ttccaaaaac
     9121 agattgataa tgtcggtgtt gagttgcatc tcacctcgtc tggcttcatc gagcaggttt
     9181 tccatcagat gcgtggtttc ctgcaaaacg ctgaagccaa aagttcctgc ccctcctttg
     9241 atcgagtggg cagcccgaaa gatggcattc aattgttcgg catctggcgc ttccggctgc
     9301 aaaaccagca gatgctgctc catatcagcc aacagttcgt ccgcttcatc aaaaaatgtc
     9361 tgataaaaat cgcttatatc gatgctcacg ctgtcacctc ggttcggctg atggcattgt
     9421 gggaacactg acctgtggtg caacctcagg tttttctagg gcgcttactg gctcattctg
     9481 gctttcggcg ttttcatgca aaatggcctg ttcggcttgt ttgttcagta ccaacaggct
     9541 gatgcgacgg ttgacggcat catcaggtcc gcgatcgctt aagcgcatcg ttgccgccat
     9601 gccgacgaca cgtaacactt tgccgctatc caaccctccg accatcagtt cgcggcggga
     9661 tgcattggcc cgatcggcag aaagctccca gttgctgtaa cctttctcac cgctggcgta
     9721 gggaaaatca tcggtatgac ctgaaaggct aatacggttg ggaataccgt tcagcacagg
     9781 cgcgatggcg cgcagaatgt cgcgcatata gggttcgaca tcggcactgc cggttctaaa
     9841 catcgggcga ttctggctat cgatgatctg aatacgtaga ccttcctgga ccagatcgat
     9901 tttgagatgg agacgtaacg cccgcagttt cggatcggac tctatcaact ggtcgagatc
     9961 accccgcaat ttccgcaatc gactttgctc catgcgtttt ttcagctctt cgatgttcgg
    10021 ctgcttattc acttccccct ggctttgggt gtaatcatca ccaccgccgg gaattgggct
    10081 ttcactattg gaaatgcgat cgccgcccgt aaccgctgtc gccagtggag tccggaagta
    10141 ctccgcaatc tgaatcaact cttttgggct ggagatggag atcagccaca tcaccagaaa
    10201 aaaggccatc atcgcagtca taaagtcggc ataagcaatc ttccacgatc catgtgctgc
    10261 cccgtggctt ttggctttgc gtcgtttgac gacaataatc ggatgcgctt gattcttcat
    10321 gcttcctcgg ttgtcgtctg ttgttgcgga tttttcaccg cacgcacatg ctcttccagt
    10381 tcaataaacg acggacgttc gctggaatag agcgttttgc gaccaaactc aacggcgata
    10441 ggcggtgcgt aaccgttcag attagaaagc agagtgactt tgacgcactg catcattttg
    10501 ctggtttcgg cgcttttctg acgtaaaaca ctcgctagtg gggaaataaa tccgtaagcc
    10561 aataaaatac cgaggaaagt ccccaccatc gcatgtgcga taagcgcccc cagctcggcg
    10621 gcaggacgat cggctgaacc taacgcgtga acgcccccca ttacagccgc gacaatacca
    10681 aacgccggaa gtgagtcccc gaccagcgcc agactgttcg ccgggacttc tgcctcgctt
    10741 tcgtgcgtct caatctcttc atccatcagg gcttcgattt cgaaggtgtt catgtgaccg
    10801 ctgataatca ggcgcagata atcgacgata aaatcaagca tgacgctatc cgcgaggatg
    10861 cgtgggtagc tggcgaagat ctcgctctca cggggatttt caatatcacg ttccagcgaa
    10921 aacatcccca tctgccgcga tttcgccatc aaccgataaa gcagagccag cagatccata
    10981 tacattgctt tggtgtattt ggagcgacga aacagcaacg gcagcgcttt cagcgtgcct
    11041 ttaatcgctt tgccattatt gccgacgata aacgacccaa tccctgcacc ggcaataatc
    11101 accagttcag cgggttgata gagtgctcca aggcttccac cggtcatcaa ataaccgccg
    11161 aaaactgtac cgagaacaac caggtaacct aataagataa gcacgacatc atccttccac
    11221 tgttgaccat gacaggatgt tcagtcgtca ggcgttaacg cgcgattggg gaaaaaaaaa
    11281 gcagcggtac gtcgttaccg ctgctggaat gttgcgccac accgtatcag ttaaacagcc
    11341 tgtactctct gttcatccag cagttgtggg ataatatcgg caggattctg ggaaagttta
    11401 cgtcttttta ctgcccggga tggcggttga cataagctgc aggcaaagct gccaacaggc
    11461 tggtgagcgt gggtaataaa attgccgccg cagcagttgc agctggaaag ttgcagtaat
    11521 ccactttcaa caaaccgcac caatgtccag gcacgggtta atgccagcag tggtccttct
    11581 tctgcttgtg ggcactgttc aaggtataaa cggtaggctt tgatcaccgc atcgacgcca
    11641 ttacacaaac cggttttcag taaaaactgc catgcattac agaacatcga agcatgaacg
    11701 ttttgttccc aggtcataaa ccagtcggtt gagaatggca gcatgccttt cggcggtggg
    11761 cttccgcgca gttctttata aagttttatc aggcgtccgc gacttaactg tgtttcgctt
    11821 tccagcatct gcaaacgagc gcccagggtg atcaattcca ttgccagctg aatatcccgc
    11881 gcttcctgaa caatgctttt ttcactcatg atcaggccct tttcttgcgc agcgcttctt
    11941 caggctgatt aacatcattc agcaagcgtg ttgagagcat gatgccggta tgaatttgct
    12001 ggagatcgtc aacgcgggaa tcttgcgtca actgagtaat cgtctggtgg ctgtcaaaac
    12061 ggaagtgaca aaccagctga ttggtttctg ccagcttaac catttgcgga aga
//
LOCUS       DAEBXN010000119        12069 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.119, whole genome shotgun sequence.
ACCESSION   DAEBXN010000119 DAEBXN010000000
VERSION     DAEBXN010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12069
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.119"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            271..1041
                     /locus_tag="KJL99_003450"
     CDS             271..1041
                     /locus_tag="KJL99_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HBD4438047.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1032..1505
                     /locus_tag="KJL99_003451"
     CDS             1032..1505
                     /locus_tag="KJL99_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HBD4438048.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISSVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1612..2151
                     /gene="dnaT"
                     /locus_tag="KJL99_003452"
     CDS             1612..2151
                     /gene="dnaT"
                     /locus_tag="KJL99_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HBD4438049.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2154..2891
                     /gene="dnaC"
                     /locus_tag="KJL99_003453"
     CDS             2154..2891
                     /gene="dnaC"
                     /locus_tag="KJL99_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HBD4438050.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2940..3434
                     /locus_tag="KJL99_003454"
     CDS             2940..3434
                     /locus_tag="KJL99_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HBD4438051.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3674..5965
                     /gene="opgB"
                     /locus_tag="KJL99_003455"
     CDS             3674..5965
                     /gene="opgB"
                     /locus_tag="KJL99_003455"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HBD4438052.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQKTLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDSCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6103..7125)
                     /gene="lgoD"
                     /locus_tag="KJL99_003456"
                     /pseudo
     CDS             complement(6103..7125)
                     /gene="lgoD"
                     /locus_tag="KJL99_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
     gene            7264..8178
                     /locus_tag="KJL99_003457"
     CDS             7264..8178
                     /locus_tag="KJL99_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBD4438053.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSQLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPRETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8393..9754
                     /locus_tag="KJL99_003458"
     CDS             8393..9754
                     /locus_tag="KJL99_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD4438054.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLNFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IITGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(9803..11467)
                     /gene="tsr"
                     /locus_tag="KJL99_003459"
     CDS             complement(9803..11467)
                     /gene="tsr"
                     /locus_tag="KJL99_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HBD4438055.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQPTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGITFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAVREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11586..12032)
                     /gene="hpaR"
                     /locus_tag="KJL99_003460"
     CDS             complement(11586..12032)
                     /gene="hpaR"
                     /locus_tag="KJL99_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HBD4438056.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
BASE COUNT         2946 a         3186 c         2925 g         3012 t
ORIGIN      
        1 ttaacttgtt aacactaaaa aacgcgccca gctatctatt atcctgaccc gtaaaggcta
       61 aacaataaat tcattatagt gataatattc atgtgatagt ttgtttgcgg cgagagataa
      121 ttcgcttttt atcaccgagc ttgtcccaat aaccaggatt aatcagcgca attttgatct
      181 taacctaccg caaaacaacc gtgcttcatg atacaaatgg attctcatta tctgaacaat
      241 acgcagcacg tctatgacaa agggcgagtt atgcaaactg agcaacagcg agccgtaacg
      301 cggctttgta tccagtgtgg attatttctt ttgcaacatg gtgcggaaag cgcgttggtt
      361 gatgagcttt cctcacgact gggtcgggcg ctgggaatgg acagcgtcga aagttctatc
      421 tcttcgaacg ccatagtgct gacaactatt aaagatgggc aatgcctgac atcgacacgt
      481 aaaaatcacg atcgcggcat taatatgcat gtggtgactg aagtccagca cattgtgatt
      541 cttgcggagc atcatctgct ggattacaaa ggcgtagaga aacgatttag ccaaattcaa
      601 ccattacgtt acccaagatg gctggtagcc ttaatggttg gcctttcttg cgcctgtttc
      661 tgtaaactca ataacggtgg ctgggatggt gccgtcatca ccttctttgc cagtacgacc
      721 gcgatgtata tccgccagct gctggcacaa cgtcatcttc atccacagat caacttttgc
      781 cttaccgctt tcgccgccac caccatttcc ggattgcttt tgcaactccc cactttcagc
      841 aataccccca ccattgcgat ggccgccagc gttctgctgc tagtgccggg ctttccgttg
      901 attaatgccg tcgccgatat gtttaaaggc cacatcaata ccggactggc acgctgggcg
      961 atcgccagtc tgctgacact ggctacctgc gtcggcgtag tgatggcact gacgatttgg
     1021 gggctacgcg gatgggtgtg atcgaatttc tgttagcgtt ggcgcaggat atgatcctcg
     1081 ccgccattcc tgcggtcggc tttgcgatgg tgttcaacgt tcccgtgcgg gcgttacgct
     1141 ggtgtgcgct gcttggctcg ataggtcatg gttcccgaat gatcttgatg accagcgggt
     1201 tgaatattga gtggtcaacc tttatggctt ctatgctggt cggtaccatt ggtattcaat
     1261 ggtcgcgctg gtatctggcg catccgaaag tgtttaccgt ggcggccgtt atccctatgt
     1321 tcccgggcat atcggcttat accgcaatga tttcgtcggt aaaaatcagc cagttaggtt
     1381 acagcgaacc gttgatgatt accctgttaa ccaactttct tacagcttca tcgattgttg
     1441 gtgcgttatc catcggtctt tccattcctg gattatggtt gtaccgcaag cgccctcgcg
     1501 tataaaattg cctcgcttaa gcacacggat gagagacagc ctcctctcct ccgtgtgtta
     1561 ctataaaagt tatctccctt ctcgttcatc gttccatatt tgagaaacag tatgtcttcc
     1621 agagttttga ccccggacgt cgttggtatt gacgccctgg tacacgatca ccaaaccgtt
     1681 ctggcaaaag ctgaaggcgg tgtggttgcc gtatttgcta acaatgcccc ggcgttttat
     1741 gccgtcacgc ctgcacgcct ggctgaactg ctggcgctgg aagaaaagct agcgcgtccg
     1801 ggaagcgatg tcgctctgga cgatcaactc tatcaggaac cgcaagccgc tcccgttgct
     1861 gtacccatgg ggaaattcgc catgtatccg gactggcaac ccgatgccga ttttatccgc
     1921 ctggcggcgc tatggggcgt ggcgctaaga gagccggtga ccaccgaaga actggcctca
     1981 ttcattgcct actggcaggc ggaaggtaaa gtctttcacc atgtgcagtg gcaacaaaaa
     2041 ctggcgcgca gcctgcaaat cggtcgtgcc agcaacggcg gactgccgaa acgagatgtg
     2101 aatacggtca gtgaacctga cagccaaatt ccaccaggat tcagagggta acgatgaaaa
     2161 acgttggcga cctgatgcaa cgcctgcaaa aaatgatgcc tgcccatatc aaacctgcat
     2221 tcaaaacggg tgaagagcta ctggcctggc agaaagaaca aggggcgatc cgctccgccg
     2281 ctctcgaacg tgaaaatcgg gcgatgaaaa tgcagcgcac ctttaaccgc tccggtattc
     2341 gtccactgca tcagaactgc tcctttgaga actatcgcgt tgagtgtgaa gggcagatga
     2401 atgcgttaag caaagcgcgc cagtatgtcg aagagtttga cggcaacatc gccagcttca
     2461 tcttttctgg taagccagga accggcaaaa accatctggc ggcggcaatc tgcaacgagc
     2521 tgctgctacg cggtaaatcc gtattgatca tcaccgtggc cgatattatg tcggcgatga
     2581 aagatacctt caggaatagc ggtaccagcg aagaacaact gcttaacgat ctgagcaacg
     2641 ttgatctgct ggtgatcgat gagatcggcg tgcagaccga atcgaaatac gaaaaagtga
     2701 tcatcaacca gatcgtcgat cgccgctctt cttccaaacg cccaaccggg atgctgacca
     2761 acagtaatat ggaagagatg accaagttgc tgggcgaacg cgtgatggac cgtatgcgcc
     2821 tgggtaacag tttatgggtg atcttcaact gggatagcta ccgtagccgg gtaacgggta
     2881 aagagtatta atccccgttt ctggcggtat actgcgacac aattccacct tgagtaatga
     2941 tgatgaaaac tattaaacat cttctgtgct gtgccattgc cgccagcgca ttaatttcca
     3001 ccggggtgca tgctgcgtcc tggaaagatg cgctctccag cgccgccagc gaacttggca
     3061 accaaaacag cacgacacag gaaggcggtt ggtcgctcgc gtcattaact aacttgctta
     3121 gcagcggaaa ccaagcctta agcgcagata acatgaacaa tgccgcaggc attctgcaat
     3181 actgcgcgaa gcaaaagctg gcttcggtaa ccgatgccga aaacatcaag aaccaggtgc
     3241 tggaaaagct gggcctgaac agtgaagagc aaaaagaaga caccaactat ctggacggta
     3301 ttcagggttt gctgaaaaca aaagatggtc agcaactcaa tctggataac atcggaacga
     3361 ctccgctggc agaaaaggtg aaaaccaaag cctgcgatct ggtgttaaag caggggctga
     3421 acttcatttc ctgaaccgcg aaatgttacc cggctgacgc ggctctctcc tgcccttttc
     3481 cctgctcctg gacggttttc cccctgttac acgggaagcg accgcgatca aaaaagtctg
     3541 tttataaact aattcctaac aacatcgcat ttttttgaca ctagaattgc agcaatatgg
     3601 cgacgccatt acattgtgct tctcaaaaag cttaaacgtt ggctaaacgg cccggtttag
     3661 tgaggatcgc ccattgtcag aactactctc tttcgccctt tttctcgcct ctgtgctgat
     3721 ttacgcatgg aaagcgggac gtaacacctg gtggtttgca gccacgttaa cggtgctggg
     3781 gctatttgtc gttttaaata tcaccctgtt tgccagcgac tattttactg gcgatggtat
     3841 taacgacgcg gttctctata ccttaaccaa cagcctgacc ggtgctggcg tcagcaaata
     3901 catcctgccg ggtatcggca ttgtgctggg gctgacggcg gtgttcggtg cgctgggctg
     3961 gatcctgcgc cgtcgtcgtc atcatccgca ccattttggt tacagcctgc tggcgctctt
     4021 actggcgctg ggttcggtgg acgccagccc ggcatttcgt cagataacgg aactggtgaa
     4081 atcccagtca cgcgatggcg acccggactt tgcggcttat tataaagagc cgtcgaaaac
     4141 tatccctgac ccgaaactca acctggttta tatctacggc gaaagtctcg agcggaccta
     4201 ttttgataac gaagctttcc cggatctcac gcctgaactg ggcgcgttga aaaatgaagg
     4261 cctggatttc agccacacgc agcagctgcc aggaacggat tacacgattg cgggcatggt
     4321 ggcttctcag tgcggcatac cgctgttcgc cccctttgaa ggcaacgcct ccgcctctgt
     4381 ctccagcttc ttcccgcaga acatctgtct gggcgatatc ctgaaaaact cgggttatca
     4441 gaactatttc gtgcagggcg caaatctgcg ttttgccggt aaagatgtgt tcctgaaatc
     4501 gcacggcttc gaccacttat acggctcaga agagctgaaa agcgtggtag ccgacccgca
     4561 ctatcgcaac gactggggat tctacgacga taccgttctc gatgaagcgt ggaaaaagtt
     4621 tgaagagctt tcccgctcag gtcagcgatt ctcactgttt accctgacag tcgataccca
     4681 tcacccggat ggttttatct cgcgcacctg taaccgcaaa aaatatgatt ttgacggtaa
     4741 gccgaatcag tcattcagcg cggtaagttg cagccaggag aatatcgcga cgtttatcaa
     4801 caaaatcaaa gcgtcaccgt ggtttaaaga tactgtcatc gtcgtctctt ctgaccattt
     4861 agcgatgaac aacacggcgt ggaaatacct caataagcag gatcgcaata acctgttttt
     4921 tgtcattcgt ggcgacaagc cgcagcaaaa gacgctggca gtgaagcgta acacgatgga
     4981 taacggcgcg acggtgctgg acattctcgg tggcgataac tatctcggac ttggtcgtag
     5041 cagtttatcc gggcagtcga tgtcggaaat cttcctcaat atcaaagaga aaacattggc
     5101 gtggaagcca gatatcatcc gcctgtggaa attccctaaa gagatgaaag agttcaccat
     5161 cgaccagcag aaaaacatga ttgccttctc gggtagccat ttccgtttgc cgctgctgtt
     5221 gcgggtttca gacaaacgcg tggaaccgct gccggaaagc gaatactcag caccgctgcg
     5281 tttccagctg gccgatttcg ctccacgcga caatttcgtc tgggttgaca gttgctacaa
     5341 gatggcacaa ctctgggctc cggaactggc actttccacc gactggtgtg tctcgcaggg
     5401 gcagcttggc ggtcagcaaa ttgttcagca tgttgacaaa acaacatgga agagcaagac
     5461 ggcatttaaa gatacggtca tcgacatggc gcgttacaaa ggcaatgtcg atacgctgaa
     5521 gattgttgat aacgatattc gctacaaagc cgacagtttc atctttaacg tcgccggtgc
     5581 gccagaagag gtgaaacagt ttagcgggat ttcccgtccg gagtcgtggg gccgctggtc
     5641 caacgcgcag ctgggcgatg aagtaaaaat cgagtacaag catccgctgc cgaagaaatt
     5701 tgacctggtg attaccgcca aagcatacgg caataacgcc agccgtccta ttccggtacg
     5761 cgtaggcaat gaagaacaaa ctcttgtgct gggcaatgaa gtgaccacca ccacactgca
     5821 tttcgataac ccaaccgatg ccgacacact ggtaattgtg ccgccggaac ctgtctcaac
     5881 caacgaaggg aatatcctcg gacactcgcc gcgtaagctc gggatcggca tggtggaaat
     5941 taaagtggta gaacgtgaag ggtaattcct gactgatgtg taggtcgcat agggtgcgac
     6001 ctacacgcct tctccggctt acaacacatt attaagaaac aaaaaaaaca gcgcccaaca
     6061 cttccccgga cgctgcatgg caactttaat aatctcgcct tatcagaaag taattacacc
     6121 cttaatcaac tcacgattgt taatcacatc gcgctcgtag gtttctgcca gcgtggcgaa
     6181 cggatagcga tgggttaaca tcatgtcggc agtgattttc ccttccgcca tcagtcgacc
     6241 gactttagcg aaatcttccg gcgtggcgtt gcggctgccc atcatcgtcg tttctttttt
     6301 atggaattcc ggatcggaga actgcaactc acctttaaac agaccgacaa ataccaccgt
     6361 gccgccgtga cgaatcagat tcacggtatt attcatcgca tgttgattac ctgtcgcgtc
     6421 gatcactttc tgcgccagcg aaccaccaaa ctgcgcccgc agctgcgcgt caaaatcttc
     6481 ggctgacggg tccagcacgg gcaactccag acgcgttgcc acatgttcac ggcgcgccgg
     6541 actggtatcc gccaccacca cctgtgcgcc atcggcttta gcgattgccg ccgcgcccag
     6601 accgattggc cccgccccga ccaccagcac ctgctcgccg ggagcaatgg ctgcgcgacg
     6661 caccgcatga gcgctaatag cgaaaggttc aatcaatgcg gccgcctgcg ggtcaatacc
     6721 gtctgcgggc aaaatgttcg ccaccggcac gctcaaatac tcactaaaac cgccatcctg
     6781 atgcacgcca atgactgaaa ttttttcaca gcagttggta cgcccgcttt tacacgccgg
     6841 gcattgctga caggcaacat aagggatcac agcaacttgc tgaccatttt taagatcagc
     6901 aatattttta cccagcccaa caatctcccc acatatttca tggcctaaaa cccgtggata
     6961 actaaaaaat ggttgatttc caccctaggc atgaatatcg gtaccgcaaa tcccgacaga
     7021 cttaattttt attaatgctt cattgtcacc cggaataggt atctcgcgtt gtttccagac
     7081 taattctttc ggctgctggc aaattaaaac attcatcgta gacatgattc ggctatccat
     7141 tttttgttgc cttagatatc aatgaaaatg aaaaatggca gcggaacgga aaatctgttt
     7201 tgtgaaaaca cacgcataaa aatgttttaa atcgggtttt aatggactca caaggaggaa
     7261 aatatgagtc gttcacaaaa tttacgccac aatgtgatta accaggtcat tgatgatatg
     7321 gcgcggggtc atatcccttc cccacttcca tcgcaaagtg cgctggcgga aatgtacaac
     7381 atcagccgca ctacagtgcg tcatattctc agccaattac gcgaatgcgg cgtcttaacg
     7441 caggttggaa acgactatgt tattgcgcgt aaacctgacc atgatgacgg tttcgcctgt
     7501 actaccgctt caatgagtga acaaaacaaa gtgtttgagc aggctttttt cacgatgata
     7561 aaccagcgtc agttacgccc tagggaaact ttttctgaac tgcaactggc tcgggccgcg
     7621 ggtgtcagcc cggtggttgt aagagaatat cttttaaaat tcggccgtta taatcttatt
     7681 caaagcgaaa agcgcggcca gtggagcatg aagcaattcg atcaatccta cgccgagcag
     7741 ctgtttgagc tacgcgaaat gctcgaaaca cattcacttc agcactttct caacctgcct
     7801 gatcatgatc cccgctggct gcaggcgaaa accatgctag aacgtcatcg gctactgcgt
     7861 gacaacatcg gcaatagctt tcgcatgttc tcgcaacttg acagagattt tcactcctta
     7921 ctgctttcag ctgccgacaa tatctttttt gatcaatcgc ttgagatcat ctcagtcatc
     7981 tttcattttc actatcaatg ggacgaaagc gatcttaaac agcgcaatat cattgccgtc
     8041 gatgaacata tgaccattct cagcgctctg atttgccgca gtgaccttga tgccaccctt
     8101 gcgctccgta accatttgaa ttcagctaaa caatcaatga ttcgatccat taatgagaac
     8161 acgcgttatg ctcattaagt acggattaaa aacagcaaac tattagcggg tgcacggttt
     8221 tatcaaaaga taaataccgg gaaattcccg cttacctatg ctcacaatca gtcgactaaa
     8281 aatgtttttg tgagcggtag taaagtccta aaactttaac ctgccgttaa tgtcaacctt
     8341 gcgtaatgca caattacgta aacaattaaa attataaata caggagtccg gcgtggaaaa
     8401 agaaaatatc accctcgatc cgcgttcttc atttactcca tcttcgtcgg cagatattcc
     8461 cgtgccacca gatggattag ttcaacgcag tacccgaatt aaacgcattc aaaccaccgc
     8521 catgttgtta ttattttttg cggcggtaat caattatctc gaccgcagtt cgctgtcggt
     8581 agcaaattta acgattcgtg aagaattggg attaagtgcc accgaaatcg gcgctttgct
     8641 ctccgtgttt tcactcgctt acggcattgc gcaacttcct tgcggcccac tattggatcg
     8701 taaaggccca cgcctgatgc tgggactggg gatgttcttc tggtcactgt tccaggccat
     8761 gtctggcatg gtgcacagct ttacgcagtt cgtgttggtg cgtatcggta tggggattgg
     8821 tgaagcgccg atgaacccat gcggtgtaaa agtcattaac gactggttca acatcaaaga
     8881 gcgcggacgc ccgatgggct tcttcaacgc agcttctacc attggcgttg ctgtaagccc
     8941 accgattctg gcggcaatga tgctggtgat gggctggcgc gggatgttta ttaccattgg
     9001 tgtactgggg atttttctcg ccatcggctg gtatatgctc tatcgcaacc gcgagcacgt
     9061 agaactgact gccgttgaac aagcttatct caatgcaggt agcgtcaatg cccgccgaga
     9121 tccgctcaat tttgccgaat ggcgcagcct gttccgtaac cgcacaatgt ggggaatgat
     9181 gctcggattc agtggcatca actacactgc gtggctgtat ctggcctggc ttcctggtta
     9241 cctgcaaaca gcctataacc tggatttaaa aagcacaggg ttgatggcgg ctatcccttt
     9301 cctgtttggg gctgccggga tgctggtcaa cggttacgtt actgactggc tggtcaaagg
     9361 gggaatggct ccgattaaaa gccgtaagat ctgcattatt accgggatgt tctgttctgc
     9421 cgcctttacg ctgatagtac cacaagcgac aacatccatg atggcggttc tgctgattgg
     9481 catggcactg ttctgtattc actttgccgg aacatcctgc tggggcttga tccacgtcgc
     9541 agttgcttct cgcatgactg cgtcggtggg cagtatccag aactttgcca gcttcatctg
     9601 cgcctctttc gcgccgatca ttactggttt tattgttgat acaacccact cattccgtct
     9661 ggcactaatc atttgcggtt gcgtcaccgc agcgggagca ctggcgtaca tcttcctggt
     9721 tcgccagccg atcaacgacc cgcgtaaaga ttaaactccc tatgcccgat aacattttgc
     9781 ttatcgggca tttttatggc gattaaaacg tttcccagtt ctccccgcta tctgccactg
     9841 ccattttacg cggcgtagct ggcgtcacgg tttttaccac agccgatgtt tcacgctgct
     9901 gttgctgctg ttgctgaatc cggaacactg ccacagcttc ggtcaggcga ctggcctgct
     9961 cttccagcgc ggcggcggca gcggcagatt cttccaccag cgcggcgttc tgttgagtta
    10021 cccggtccat ctcagcaacc gctaagccaa cctgatcgat accacggctc tgctcatcag
    10081 aagcagaagc tatttcgccc ataatgtccg tcacgcgggt cacagcactg acaatctccg
    10141 ccattgtttc cccggcgctt tcgaccagcg tagagccaac atccactttc cccaccgagt
    10201 cttcaatcag gcttttaatt tcacgaaccg cctgggcgct acgctgggcc agattacgca
    10261 cttctcccgc aaccaccgca aaaccacgtc cttgctcacc cgcacgcgct gcttcaaccg
    10321 ccgcgttcaa agccagaata ttggtctgga aggtaatgcc gtcaattacg ctgataatat
    10381 cggcgatttt ctgcgaactg gtggagatat cgcgcatagt ctgcaccacg ttatctacca
    10441 ctttgccgcc gcgttgcgcc gtttcagaag cacttaatgc cagatggctg gcctggcgcg
    10501 cattctcggc gttctgtttc accgttgcgg tcagttgctc catgctggct gccgtctctt
    10561 ccagcgaagc ggcctgttgc tcggtgcgcg aagagagatc attattgccg gtagcgattt
    10621 cgctggcacc gctatagatg gcattggccc cgttgcgcac atcaccgacg gtacgcatca
    10681 gctctccctg catatggcgc aaactctctg ccagttgccc catctcatta gagccatcca
    10741 cctcaatcgg tttcaccaga tcgccgcctg caatatgacg aatgctgtca atcaggcgat
    10801 tcattggcgc taccagcgag gctttaatac cgaaccagac ggcgaagatg accgccagta
    10861 cgacgatcat cacgcccacc agaatccaca tcgcctggct gtaggaggca ttgttatcgc
    10921 tgacggcgat atcatagagc cgatcgtttt gctccatgta agccacatac tgcttctcga
    10981 aaccgtcctg atatccctgg gtcggctgat caaagaactc gttgattttg cctgcaccta
    11041 acagttggat cagctccgcc agcgcattgt gataaatatc gtaattacgt ttgatctctg
    11101 ccgctgcggc ggtgctctga cgcgggtcac gcggcaacgc ttcgtaatcc gcccagtttt
    11161 tttccgcctg tttcagcgaa atactggcac tctgcatcag ctcagcaacg gttgaaccgc
    11221 taccaatatt attctgatcc atcatgtagc ggatacccgc gcggttgagg gtgttacgcg
    11281 tctgcaacaa cgcgacccag ctgccattca gcgtgggttg ctgctggcga atggtttgta
    11341 aaacagtgaa attttctttg tcattcttta aggcattaaa aaacagaccg cctgatgtca
    11401 gttgtaaaag gccaaaaacg gccaaaacca gcagtaagct ggtcacgatc ttgatacgtt
    11461 ttaacatggt ttctctttcc tgtggaccga tgcaagattt tcggcctgga aagcaaaaac
    11521 tttatgaaaa ggtgtaaccc gccatccatg gaaaaaccag gcgctggtta tcagatacta
    11581 aaaagttatt cattatcgcc cgggatgtct tcttgtcggg aattgcccag agcaataaat
    11641 tcctctaaca gatgggttaa ctgttgcatt ttctcggcag taaactgagc ttcaatctgc
    11701 cgataggctt cttcaatctg tgtctgagcg cggttataca gcgcctgccc ctctttggtc
    11761 agcgagatat agagcttccg ctgatcattg atcggcttta atcgcaacac taaaccgtcg
    11821 cgttccatcc gcgtgaggat cccggtcagg cttgggcgca aaatgcaggc gcgatacgcc
    11881 agatcgtgaa aatccattga tgggctttcc gccagaatac gcacgatgcg ccactgttgc
    11941 tcagtcaaat tatgccgttt aacgattggg cgaaaataac tcatcgccgc ttctcgcgcc
    12001 tggagcaacg caatggttag tgagtcgtgc attatctttc ccctgaaaaa ttcaaaatca
    12061 ttaatatag
//
LOCUS       DAEBXN010000120        11923 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.120, whole genome shotgun sequence.
ACCESSION   DAEBXN010000120 DAEBXN010000000
VERSION     DAEBXN010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11923
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.120"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(<1..2340)
                     /locus_tag="KJL99_003461"
     CDS             complement(<1..2340)
                     /locus_tag="KJL99_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HBD4438057.1"
                     /translation="MDTQRFQSQFHWHLSFKFSGAIAACLSLSLVGTGLAKADDSLPS
                     SNYAPPAGGTFFLLADSSFSSSEEAKVRLEAPGRDYRRYQMEEYGGVDVRLYRIPDPM
                     AFLRQQKNLHRIVVQPQYLGDGLNNTLTWLWDNWYGKSRRVMQRTFSSQSRQNVTQAL
                     PELHLGNAIIKPSRYVQNNQFSPLKKYPLVEQFRYPLWQAKPVEPQQGVKLEGASSNF
                     ISPQPGNIYIPLGQQEPGLYLVEAMVGGYRATTVVFVSDTVALSKVSGNELLVWTAGK
                     KQGEAKPGSEILWTEGLGVMTRGVTDDSGTLQLQHISPERSYILGKDAEGGVFVSENF
                     FYESEIYNTRLYIFTDRPLYRAGDRVDVKVMGREFRDPLHSSPIVSAPAKLSVLDANG
                     SLLQTVDVTLDARNGGQGSFRLPENAVAGGYELRLAYRNQVYSSSFRVANYIKPHFEI
                     GLALDKKEFKTGEAVSGKLQLLYPDGEPVKNARVQLSLRAQQLSMVGNDLRYAGRFPV
                     SLEGSETVSDASGHVALNLPAADKPSRYLLTVSASDGAAYRVTTTKEILIERGLAHYS
                     LSTAAQYSNSGESVVFRYAALESSKQVPVTYEWLRLEDRTSHSGELPSGGKSFTVNFA
                     KPGNYNLTLRDKDGLILAGLSHAVSGKGSTAHTGTVDIVADKTLYQPGETAKMLITFP
                     EPIDEALLTLERDRVEQQSLLSHPANWLTLQRLNDTLYEARVPVSNSFAPNITFSVLY
                     TRNGQYSFQNAGIKVAVPQLDIRVKTDKTHYQPGELVNVE"
     gene            complement(2274..2897)
                     /locus_tag="KJL99_003462"
     CDS             complement(2274..2897)
                     /locus_tag="KJL99_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HBD4438058.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSRLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGSGKNGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(2894..4582)
                     /locus_tag="KJL99_003463"
                     /pseudo
     CDS             complement(2894..4582)
                     /locus_tag="KJL99_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
     gene            complement(4731..7358)
                     /gene="gyrA"
                     /locus_tag="KJL99_003464"
     CDS             complement(4731..7358)
                     /gene="gyrA"
                     /locus_tag="KJL99_003464"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HBD4438059.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7505..8227
                     /locus_tag="KJL99_003465"
     CDS             7505..8227
                     /locus_tag="KJL99_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HBD4438060.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(8355..10286)
                     /gene="yfaL"
                     /locus_tag="KJL99_003466"
     CDS             complement(8355..10286)
                     /gene="yfaL"
                     /locus_tag="KJL99_003466"
                     /inference="COORDINATES: protein motif:HMM:NF007285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HBD4438061.1"
                     /translation="MEITANVDTRSTTTEHGRDIEMRADGEVAVDAGVDTQWGALMAD
                     SSGQHQDEGSTFTKTGAGTLELTASGTTQSAVRVEEGTLKGDVADIFPYASSLWVGDG
                     ATFVTGADQDIQSIDATSSGTIDISDGTVLRLTGQDTSVALNASLFNGDGTLVNATDG
                     VTLTGELNTNLETDSLTYLADVTVNGDLTNTSGAVSLQNGVAGDTLTVNGDYTGGGTL
                     LFDSELNGDDSVSDQLVMNGNTAGNTTVVVNSITGIGEPTSTGIKVVDFAADPTQFQN
                     NAQFSLAGSGYVNMGAYDYTLVEDNNDWYLRSQEVTPPSPPEPDPTPDPDPTPDPDPT
                     PDPAPTPAYQPVLNAKVGGYLNNLRAANQAFMMERRDHAGGDGQTLNLRVIGGRYHYT
                     AAGQLAQQEDTSTVQLSGGLFSGRWGTDGEWMLGAVGGYSDNQGDSRSNMTGTRADNQ
                     NHGYAVGLTSSWYQHGNQKQGAWLDSWLQYAWFNNDVSEHEDGVDRYHSSGIIASLEA
                     GYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVSQSQGDDIQTRLGLHSEWRTAV
                     GVTPTLDLNYYHDPHATEIEEDGSTISDDAVKLRGEIKVGVTGNISQRVSLRGSVAWQ
                     KGSDDFAQTAGFLSMTVKW"
     gene            complement(10299..11894)
                     /locus_tag="KJL99_003467"
     CDS             complement(10299..11894)
                     /locus_tag="KJL99_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000183628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AidA family protein"
                     /protein_id="HBD4438062.1"
                     /translation="MTNNASGGAVFLQQGAEFSLLPENETGMTLFANNTVTGEYNNGG
                     AIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLRVTNAINNIANDGKGG
                     AIYTINNDVYLSDDVFNNNQAYTSTSYSDGDGGAIDVTDNNSDSKHPSGYTIINNTAF
                     TNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSAAGYGDGPSTAAGG
                     FMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSGDLVLNADNNDFTG
                     EMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDQYQNQAELNVGSTQQTF
                     VHALTGFQNGTLNIDAGGNVTVNQGSFAGIIEGAGQLTIAQNGSYVLAGAQSMALTGD
                     IVVDDGAVLSLEGDAADLTALQDDPQSIVLNGGVLDLSDFSTWQSGTSYNDGLEVSGS
                     SGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANNNSYLGTTQIASGT
                     LMVSDNSQLGDTHYNRQVIFTDK"
BASE COUNT         2757 a         3307 c         2944 g         2915 t
ORIGIN      
        1 ttcgacattg accagttcac caggctggta atgggttttg tccgttttca cccggatatc
       61 aagctgagga acggcaactt tgatcccggc gttctgaaaa ctgtactgac cattacgggt
      121 atacagcacc gaaaaagtga tgttaggcgc aaaggaattg ctcactggta cccgggcttc
      181 atacagggta tcgtttaaac gttgtagcgt tagccagttt gccggatgcg aaagcagcga
      241 ctgctgttcc acgcgatcgc gttccagcgt caataacgct tcatcaattg gctccgggaa
      301 agtaatcagc atcttcgcgg tttcgcctgg ctggtacagc gttttatccg ccacgatatc
      361 taccgtacca gtatgcgccg tgctgccctt accgctgacg gcatgactta acccagcgag
      421 aattaagccg tctttatcgc gtaatgtcag attgtagttg ccaggtttag cgaaattgac
      481 ggtaaaggat ttgccgcctg acggtagctc tccgctatgg ctcgtgcggt cttcgagacg
      541 caaccattca tacgtaacag gaacctgttt tgaagattcc agcgcggcat aacggaacac
      601 aaccgactcg ccgctattac tgtattgtgc ggcggtactt aaagagtaat gcgccaggcc
      661 gcgttcaatg aggatctctt tggtggtggt gacgcgatac gccgcgccgt cactggcgga
      721 gacggttaac aaatagcggc tcggtttatc ggcggcgggg agatttaacg ccacatgacc
      781 gctggcgtcg gacaccgttt cgctgccttc cagcgacacg gggaaacgtc cggcataacg
      841 caaatcgtta ccgaccattg ataattgctg agcgcgcaaa cttaactgca cgcgggcatt
      901 ttttaccggc tcgccatccg ggtagagcag ttgcagtttg ccgctgaccg cttcgccagt
      961 tttgaactct tttttgtcga gagctaaacc aatctcgaaa tgtggcttga tgtagtttgc
     1021 cacgcgaaaa ctgctgctat agacctgatt gcggtaagca agacgtaact cataacctcc
     1081 ggcaacggca ttttctggca ggcggaaact tccctgcccg ccattgcgcg catccagcgt
     1141 gacatcgacg gtttgcaaca gactgccgtt agcgtccagc accgaaagct tcgccggggc
     1201 gctgacgatg ggggatgaat gcaacggatc gcggaactcg cggcccatca ctttaacatc
     1261 gacacgatcg cctgcgcgat atagcgggcg atcggtaaaa atatacaagc gggtgttgta
     1321 gatttcgctt tcgtagaaga agttctcgga gacaaaaacg ccgccttcag catccttacc
     1381 cagaatgtat gaacgttctg gcgatatatg ttgtaactgc aaagtaccgc tgtcatcggt
     1441 caccccgcgg gtcatcacgc caagaccttc agtccacaag atctctgagc cgggcttcgc
     1501 ttcaccctgt tttttacccg cggtccatac cagaagctcg ttacctgaca ctttgctaag
     1561 cgccacggta tcggaaacaa acaccaccgt cgtcgcccga tacccaccta ccatcgcctc
     1621 gacgaggtac agccccggct cttgttggcc gagaggaata taaatattac ccggctgcgg
     1681 cgagatgaaa ttgctggatg cgccttccag ttttacccct tgctgcggct cgaccggttt
     1741 agcctgccat agtggataac ggaactgttc caccaaggga tattttttca gcggggaaaa
     1801 ctggttgttc tgtacataac gggaaggttt aataatggca ttgccgagat gtaattcggg
     1861 taatgcctga gtcacattct gccgtgactg agaagagaaa gtacgctgca tcacgcggcg
     1921 agatttgccg taccagttat cccacagcca ggtcagcgta ttgttcagcc cgtcgcccag
     1981 atattgcggt tgcaccacaa tgcgatgcag gtttttctgc tggcgcaaaa atgccatcgg
     2041 gtcaggaata cgatataggc gaacgtccac gccgccgtac tcttccatct gatagcgccg
     2101 ataatcacgc cccggcgctt ccagtcgcac tttcgcctct tcactgctgc taaaactgct
     2161 gtcagcaagc aaaaagaatg ttcccccggc gggcggcgca tagttactgg aaggaagcga
     2221 atcatcagct ttagcaagcc ctgttcccac aagagacagg gacaagcacg ctgctatcgc
     2281 gccagaaaat ttaaacgata aatgccaatg aaattgggat tggaatcgtt gggtatccat
     2341 cgggtgtcct tccatgtcat aagttgttgc agactgactg cgcgcattcc gttgtcagtt
     2401 ttcgtggcgc ttccggtgtg gtagatgacg taacgcccca tccagaccat taagtgctgg
     2461 gcatcgccct gatcgaaaaa aatcatatcg ccaggcagcg cctggtttat gtcttggcca
     2521 ataaactggc tgttgtactg aatcaaatta atcgcggtca cgtaggggcc gtttttcccg
     2581 ctcccctgat tccagttttg cgccagttga cgctgttcag gtgttagcgt catctctggc
     2641 ggcaaatact ggctagataa accgttactt tttagccatt tactgtcgtg aactttcagc
     2701 gtctcgttcg ccgcaaatcg caccaggccc gcacaatcct gctgatacca gcgtggactt
     2761 ggcccctggc ggagctgttc ttgtgcaatg cgtacaaacc aggcgcgaaa cagcctggat
     2821 tgttcgacat tcagcatttc gctatgggca acaacacaac acagccagca aatcagcgcc
     2881 agcagcccgt gtctcatagc ggctgccagg ttatgggtag ccactgccag gcgacaccgg
     2941 gttccatctg ggccagcttc atgacataac gcggttgttg agataaagcg tccagcttcg
     3001 gcattaataa agtttgtgcg gcgttataaa aaaccggttc gagattcttc ggcagactgg
     3061 tcaacgtttc gttacgcagc agtttcgcca cgccttgtgg attgatatag agcggaacga
     3121 tgccatcagt gggtattaca tccaccattg ccggacgggt tttattcagt gtttgcaggg
     3181 cgttattaac cagcgtgtca tcgagggaga aaagcagcgt tttgttttgc atcgccagcg
     3241 acacacggaa aaaataatcc gacattaatt gatcgggttg cgccgcctgc gctttcggat
     3301 actgaccgta tcgggaactc acttcgcgac accagatttg cgcttcgccc tgctgagtct
     3361 ggcttaccgg caaaacacct tctggcgctt tgctttcgtg cgcaccaata ttttgcgtaa
     3421 acagtttccc tggcaattgc gcctgttcgg cagtgccatc aaactgaccg acaaacagcg
     3481 gggtttgcaa ttttgagtct tcataccagc acagccccgc ggcaccgtct aacgccccat
     3541 tcagcttgtc gttttcctgg ctgatgtgcg aaagcatctc ttcggcaata ccgtgtgaat
     3601 acggcaccgc cacacagaag ctggctccgg caggcatact gttccagacc ggcgtaaaat
     3661 cgaaactggc atctacgctg gcggattcat catttaacgc cacaaaactg tgccagccgt
     3721 cgttacccat ttcgaagcgt acgccagcaa aagaaggcat taaccgctgg tagccaaaac
     3781 ccagcaacct ggcgctgacc acgatgcgca ggcgtactgg cgttttttcg gctgtacgct
     3841 cttccaggcc aaagctggct tgccagcgtt ttttaccgct caacaaatca cttgcgatcg
     3901 ccgtggcttc ggtatcctgc tgatcatctt taaacaacat atccgtactg gaaaacacca
     3961 gcatcttgtc ctgatatgtc gcgaacatca gggcgttatt gccgttatag cgcaactgat
     4021 aaacaggaat agtttcacta tttatcttga tgctactgat ttccgttttg cttaactagc
     4081 tgtcgctggt agcggcaaac aacaatggct ccagcagttt gcttaaacca ctgcgctgga
     4141 tcagcaccat gtaatgtgaa agatggcctt gtttatcgtg ccatagcgcc gcctgcgcgg
     4201 gctgatctaa gagtgacgaa aagagcttat ctttcagcgt gagatcgtgt tcatagacaa
     4261 tgcggcgaat gctgccttca atgcccagac gatcggcatg attctgataa tagaaaacga
     4321 aatcttcgct cagaacatcg tggagaaacg gaatggtgag gagatctttg ggaagctggc
     4381 tcagagagtc gctgtctaga aagaggtccg gctcattgag atcgatttgc agattgttgt
     4441 gcaccaccag cggcgacaac gttttttctg gcccactgcc agcatattgc aacgcccaga
     4501 cgccagcgga aagcagtgct attgcgccaa aacctacaag accatagaac cgccagcctt
     4561 tcgccttttt ttcaccactc attgccacat tccttgtgta tagccagcca ttttttacgg
     4621 gcacagccaa actttaccgc gccctaatac gacaaaagcc cagactttgc agcctggact
     4681 tttcaattca aacaagggag atagctccct tttggcatga agaagtaaaa ttattcttct
     4741 tctggctcgt cgtcaacgtc cacttccgga gcaatttcat catccccttc cgcggcactg
     4801 ccgtcgatgg tatccagatc ttcctcgtca accggttcag caacacgctg cagacccact
     4861 acgttttcat cttccgcagt acggatgagg atcacgccct gggtgttacg gcccacgatg
     4921 ctgatttctg aaacgcgagt acgtaccagc gtaccggcat cggtgatcat catgatctgg
     4981 tcgcagtcat ctacctgcac cgcgccaacc actaaaccgt tacgttcggt aaccttgatg
     5041 gagataaccc ctttcgtcgc acgcgacttg gttgggtatt ccgccactgc agtacgttta
     5101 ccgtaaccgt tttgcgttgc ggtgaggatt gcgccatcgc cacgaggcac gatcagagag
     5161 acgactttat cgccttcacc taagcgaata ccgcgcacac cagtggtatt gcagcccatc
     5221 gcacggacag aagactcttt aaagcgcacc actttacctt cggcggagaa cagcattact
     5281 tcatcttcgc cactggtcag atcaacgccg atcagctcat cgccgtcaac cagtttgatc
     5341 gccactttac cggcggtacg cagacggttg aattcggtga ggacggtttt cttcacagta
     5401 ccgttagcgg tcgccatgaa gactttcacg ccttcttcaa actcggtcac cggcaggatc
     5461 gcggtgatac gttcgtcctg ttccagcggt agcaggttga cgatcggacg accgcgcgcg
     5521 ccacgagtgg cttccggcaa ctggtaaact ttcatcgaat agacgcgacc acggctggag
     5581 aagcacagaa tatggtcgtg cgtgttcgcc accagcagtc ggtcgataaa gtcttcttct
     5641 ttaatacgtg cggcagattt acctttcccg ccacgacgct gcgcttcgta ttcagaaagc
     5701 ggctgatatt taacgtagcc ctggtgagag agcgtcacga ccacatcttc ctgggtgatc
     5761 agatcttcca ggttgatgtc tgcgctgttg gcggtgattt cagtacgacg tttgtcaccg
     5821 aactgttcac gaaccagctc cagctcttcg cggatcactt ccatcagacg atcggcgcta
     5881 ccaagaatac gcaacagttc cgcgatctga tccagcagct ctttgtattc gtcgagcagt
     5941 ttttcgtgct caaggccggt cagtttctgc aaacgcagat ccagaatcgc ctgagcttgc
     6001 tgttcggtca ggtagtacag accatcacgc acgccgaact ctggctccag ccattccgga
     6061 cgcgcagcat cgtcgccagc acgttccaac atggcggcaa cgttgcccag ctgccacgga
     6121 ttagcaacca gcgcagtttt cgcttctgca ggcgtcggcg catgacggat cagttcgatg
     6181 atcgggtcaa tgttcgccag cgccacggct aatgcttcaa ggatatgagc acgatcgcga
     6241 gctttacgca gttcgaaaat agtacgacgg gtcaccactt cacggcggtg acgaacaaac
     6301 gccgcgatga tgtctttcag gttcatgatc ttcggctgac catggtgcaa tgccaccatg
     6361 ttgataccga aagaaacctg caactgggtc tgggagtaga ggttgttgag cacaacttca
     6421 ccgaccgcat cgcgtttcac ttcaatcacg atgcgcatac cgtctttgtc agactcgtca
     6481 cgcagcgcgc tgatgccttc cacgcgtttt tcttttacca gttccgcaat cttctcgatc
     6541 aggcgcgctt tgtttacctg atacggaatt tcgtggacga taatggtttc acgtccggtt
     6601 ttggcgtcaa cttccacttc agcacgggcg cggatatata ccttgccgcg accggtacgg
     6661 taagcttctt caataccgcg acgaccgtta atgattgccg ccgtcgggaa gtccggcccc
     6721 gggatgtgtt ccatcagccc ttcaatgctg atgtcttcat catcgatata cgccagacaa
     6781 ccgttgatga cttccgtcag gttgtgcggc gggatgttgg ttgccatacc tacggcgata
     6841 ccggaagaac cgttcaccag caggttagga attttggttg gcatgacgtc gggaattttt
     6901 tccgtgccgt catagttatc aacgaaatcg accgtctctt tttcgagatc ggccatcagt
     6961 tcatgggcaa ttttcgccag acggatttcc gtataacgca ttgccgccgc agagtcgccg
     7021 tcgatggaac cgaagttacc ctgaccgtct accagcatgt aacgcagcga gaatggctgc
     7081 gccatacgga cgatcgtgtc ataaaccgcc gagtcaccat ggggatggta tttaccgatt
     7141 acgtcaccaa cgacacgggc agatttttta taggctttgt tccagtcatt gcctagtacg
     7201 ttcatggcgt aaagtacgcg acggtgtacc ggcttcaggc catctcggac atctggcagc
     7261 gcacggccaa caatgaccga catcgcataa tccagatagg agctctttag ctcttcctca
     7321 atgttgaccg gtgtaatttc tctcgcaagg tcgctcatct aaccgctatc cctctactgt
     7381 atcccggatt caaaggtcgc aaattataac acagccgcgc agtttgaggt aaacctatac
     7441 gctttattca catccaatgc ctgatatact cgtttgtctt gccaattacg gagtagaagt
     7501 gccaatgaat gccgaaaaat cgccggaaaa ccataacgta gaccacgaag agatcgctaa
     7561 atttgaagcc gtcgcctccc gctggtggga tcttgaaggt gagttcaaac cgttgcaccg
     7621 cattaacccg ctgcgtctgg gctatattgc cgagcgtgct ggcggtttat ttggcaaaaa
     7681 ggtgctcgat gtcggttgtg gcggcggcat tctggccgag agtatggcgc gcgaaggcgc
     7741 gacggtgacc ggtctggata tgggctttga gccattgcag gtggcaaagc tgcacgcgct
     7801 ggaaagcggc attcaggtgg attacgtgca ggaaaccgtg gaagagcacg cggcaaaaca
     7861 tgccgggcag tatgatgtgg tgacctgcat ggagatgctg gagcacgtac ccgatccgca
     7921 gtcagtggtc agagcctgtg cgcaactggt gaaaccaggc ggcgatgtct ttttctcgac
     7981 gcttaaccgc aacggcaagt catggctgat ggcggtggtt ggcgcggaat atattttgcg
     8041 catggtgccc aaaggtacgc atgatgtgaa gaagtttatt aaaccggcag agctgcttgg
     8101 ctgggtggat cagaccagtt taaaagagcg acatataacc gggctacatt acaacccgat
     8161 cactaattcc tttaagctcg gccccggcgt ggatgtgaac tatatgctgc acacgcagaa
     8221 taagtgaggt tgatgtttgg ccgcgccaat gcctgatgcg acgcttgcag cgtcttatca
     8281 ggcctacaaa tgctctccgt aggccagata aggcgtttac gccgcatccg gcaattgtga
     8341 tggtcagaca gcgattacca tttcaccgtc atcgacaaaa accctgccgt ctgggcaaaa
     8401 tcatcactcc ctttctgcca cgccacgctg ccgcgcaggg acactcgctg actgatattg
     8461 cccgtgactc ccacttttat ttcaccccgt agcttcaccg catcgtcact gatagtgctg
     8521 ccatcttctt caatttccgt cgcatgggga tcgtgatagt aattcagatc taatgttggt
     8581 gtgacaccaa ccgcggtacg ccattcgctg tgtaaaccca gccgcgtctg aatatcatcc
     8641 ccctgcgatt gtgacacgcg cgcatggtta gcggcggtaa aatcatcctg ctgcacgccc
     8701 tgataaatca cctgcgcctg cggttcaatc accacaccac gccccggtaa ccactgatac
     8761 ccagcttcca gcgaggcgat aatccccgac gaatggtaac gatccacgcc atcttcatgt
     8821 tcggaaacat cattgttaaa ccacgcgtat tgcagccagc tatccagcca ggccccctgc
     8881 ttctgattac cgtgctgata ccagcttgag gtcagcccaa cggcataacc gtggttctgg
     8941 ttatcggcgc gagttccggt catattcgag cggctgtcgc cctgattatc actgtagcca
     9001 ccaaccgccc caagcatcca ctcgccatcc gtgccccagc gcccgctaaa caagccgccg
     9061 ctaagctgca ccgtagaagt gtcttcctgc tgagccagtt gccctgccgc cgtgtaatga
     9121 taacgtccgc cgataacacg taaattcagc gtctgaccat cgccaccagc gtgatcgcgt
     9181 cgctccatca taaacgcctg atttgccgcc cgcaggttat tgagataacc gccaactttg
     9241 gcattcagca ccggctggta agcaggcgta ggggccgggt cgggggttgg gtcaggatcc
     9301 ggcgtgggat caggatcggg agtcgggtct ggctcaggtg gcgatggcgg cgttacttct
     9361 tgcgatcgca gataccagtc gttgttatct tccactagcg tgtagtcata cgctcccatg
     9421 ttgacgtagc cactgccagc cagactgaac tgtgcattgt tttgaaactg cgtgggatca
     9481 gctgcgaaat caaccacttt aatgcctgtc gatgtcggct caccaatccc tgtaatggag
     9541 ttaaccacca cagttgtgtt gccagcagta ttaccgttca tcaccaattg atcgcttacc
     9601 gagtcatcgc cgtttaattc gctgtcgaat agtagcgtac cgccgccggt ataatcaccg
     9661 tttaccgtca gcgtatcgcc agcgacgcca ttttgcaggc taaccgcacc ggacgtattg
     9721 gtcagatcac cattaaccgt cacgtcagca agataggtca ggctgtcagt ttcaaggttg
     9781 gtattaagct cgcctgtcaa cgtcacgcca tcggtagcat ttaccagcgt cccatcgccg
     9841 ttaaatagtg aggcattaag ggcgacggaa gtatcctgcc cggtcagacg caaaaccgta
     9901 ccatcgctga tgtcgatagt gccgctggaa gtagcatcaa ttgactgaat atcctgatcc
     9961 gcgccagtaa cgaacgttgc cccatcacca acccacagtg acgaagcata aggaaagata
    10021 tccgcaacat cgcctttcag ggtgccttct tcgacacgta ccgccgactg cgttgtaccg
    10081 ctggcggtca gctccagcgt acccgccccc gttttagtaa atgtgctacc ctcatcctga
    10141 tgctgcccgc tgctgtcagc catcagtgcg ccccactgcg tgtctacccc cgcatcaact
    10201 gccacttcac cgtcggcgcg catttcaata tcacgcccat gctcagtcgt cgtagagcga
    10261 gtatcgacat tggcagtaat ctccatcacg ctttcttgtt acttatcggt aaagataacc
    10321 tggcggttat agtgggtatc tccaagctgc gagttgtcgc tcaccatcag cgtaccggag
    10381 gcgatttgtg ttgtgcccaa ataactatta ttgtttgcca gacttacctg cccgtcgctc
    10441 gcatcgacca ccacgtagac gccatctttc ccgtcgccgc cgatatgcaa attgtcgcca
    10501 cctgcaagat ctaccacatc ctgactgccg ataaccgttc cgctgctgcc actgacttca
    10561 aggccatcat tgtatgatgt gccgctctgc caagtggaga aatcagagag atcgagcaca
    10621 ccgccgttta acacgatcga ctgcggatcg tcctggagag cggtaagatc tgccgcgtcg
    10681 ccttccagcg aaagcaccgc accatcatca acgactatat cgccggttag cgccatcgac
    10741 tgcgcccctg ccagcacgta gctgccgttt tgcgcaatgg tgagctgacc agcaccttcg
    10801 atgatgccag caaaactgcc ctgattaaca gtaacgttgc caccagcatc gatatttaaa
    10861 gtgccattct gaaagcccgt caatgcgtgc acaaaagttt gttgggtcga gccaacgttt
    10921 agctcagcct gattctgata ctgatcaata ctccctatcg tcagaccgta gcagtcttgc
    10981 ggatcgtcct ggcaatgcgt atcgccgaca ttcatcaggg agttgctgcg acccagggta
    11041 acttcaccgt tttcaatctg catctcacca gtaaagtcat tgttatctgc attaagtacc
    11101 agatcgccgg aacctgtttt ggtgattaac ccggtaccag caatagagtc aacagctccg
    11161 tcattctctg tattgccgat aaccagcgtt tttccgtcgg caatatcaaa ggtaacttca
    11221 cttaagccga gatacataaa gccacccgcc gcagtggagg gaccatctcc atagcctgct
    11281 gcgctattgt tctcatcgac taacacgccg ccgttctggc tgtagctgtc atcaacagaa
    11341 atatcaataa gatagggagc cgtcgcgcta ttggtatata tcgccccgcc ataaccttcg
    11401 gctgtgttat ttgtaaaggc ggtgttattt attatcgtat aacctgaagg atgcttgctg
    11461 tcgctattat tatcggtaac atcgattgcc ccgccatcgc catcactgta acttgttgat
    11521 gtatatgcct ggttattgtt aaaaacatca tcacttaaat agacgtcatt attaatggta
    11581 taaattgcgc cacctttgcc atcattagcg atattattga tggcgttagt gacacgtaaa
    11641 tcgacggcac cagtatcatt agtaccagaa gaatagattg cgccaccata gccgcctgcg
    11701 acgttaccgg aaaaaataac atcagtaaga ttcagcgttg agttttcttt agcaaatatg
    11761 gccccgccgt tattatattc tcctgtaacg gtgttattgg caaacagagt cattccagtt
    11821 tcattttctg gtaatagtga aaattccgct ccttgttgca aaaatacggc cccaccgctg
    11881 gcattattgg tcatatccga aaaaaccagc cattgcccat cgg
//
LOCUS       DAEBXN010000121        11887 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.121, whole genome shotgun sequence.
ACCESSION   DAEBXN010000121 DAEBXN010000000
VERSION     DAEBXN010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11887
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.121"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            423..836
                     /locus_tag="KJL99_003468"
     CDS             423..836
                     /locus_tag="KJL99_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438063.1"
                     /translation="MFFIENEGQAVAGTDYWQSVQAQAGYVYLSWNAGAARLLVPDAA
                     KHLLREMRGAEYVIISKGALHGRDALELVFEDGSDAPFVIHMLSEQCDRLLPENNQGG
                     GFVVTVWTRGGNQLRYPGKYRVVENLPDVSPWSEH"
     gene            838..1617
                     /locus_tag="KJL99_003469"
     CDS             838..1617
                     /locus_tag="KJL99_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase family protein"
                     /protein_id="HBD4438064.1"
                     /translation="MQHLPAPIHHARDAAQLPVAIDYPAALALRQMSMVHDELPKYLL
                     APEVSALLHYVPDLRRKMLLATLWNTGARINEALALTRGDFSLTPPYPFVQLATLKQR
                     TEKAARTAGRMPAGQQTHRLVPLSDAWYVSQLQTMVATLKIPMERRNKRTGRTEKARI
                     WEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIPLKVLQSLMG
                     HKSISSTEVYTKVFALDVAARHRVQFSMPESDAVSMLKRIP"
     gene            1797..2441
                     /locus_tag="KJL99_003470"
     CDS             1797..2441
                     /locus_tag="KJL99_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBD4438065.1"
                     /translation="MPVIANTHPKGGVGKTTSSVNIVGEMKSDTVDLDTHTGLSIILG
                     LRPEGKEISVKVPKTVDELIEIMTPYKNSDKTLLIDCGGFDSDLTRTAIAFADCVIVP
                     SKDSLTERIGLMHFDGVLDEISSIMGTDITAHLYLCKVNPNKKKFPKLDAILPSFKHL
                     KLMKSRISARAEFEDVIETGMGITESVHGRYSAGGKEVIALIEEINHLIENNKQ"
     gene            2528..2836
                     /locus_tag="KJL99_003471"
     CDS             2528..2836
                     /locus_tag="KJL99_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone GroEL"
                     /protein_id="HBD4438066.1"
                     /translation="MSKAKFSAQAEKLKNLVDSARLQTSSSAVTATAPSQPNEGAEEV
                     SGRPVAARTKAKNLKAIPLSYFEAHAELKATSKTSLDFSSYIIEAIREKLERDGAIGQ
                     "
     gene            3250..4230
                     /locus_tag="KJL99_003472"
     CDS             3250..4230
                     /locus_tag="KJL99_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HBD4438067.1"
                     /translation="MKIFIDDGSTNIKLAWLEDGDVKTLISPNSFKPEWSFSLLDDAA
                     PANYEIDGEKFSFDPLSADAVVTTETRYQYSDVNVVAIQHALQQTGLKAQPVDVIVTL
                     PISEYLDANNQKNKQNIERKKKNVMREVRVQGSDAFVIRSVSVLPESIPAGFSVLAGL
                     EDDESLLIVDLGGTTLDVSHVRSKMTGITKTWCDPNIGVSLITSGVKEQMAVHANTRV
                     SSFQADNIIVHRNEPDYLSRRIYNAEQRESIINVINERQKLLIKRVNDVISRFTDYTH
                     VMCVGGGAEIVAEAVKNLTKVPDERFYLSSSPQFDLVMGMIKMKGGVTNE"
     gene            4223..4639
                     /locus_tag="KJL99_003473"
     CDS             4223..4639
                     /locus_tag="KJL99_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HBD4438068.1"
                     /translation="MSDENKSRRCSFELFPDERTGDKIADELIANEKLKERGRFMRAM
                     LVTGAAFAAIDKRLPLLISELLTENTTLDDINKVISSVIPGAFSVEKKLLELLEKQSG
                     LHTSVDCSTPLTEQSLSRNDGEDQTRRNAENMFGDD"
     gene            complement(4641..5915)
                     /locus_tag="KJL99_003474"
     CDS             complement(4641..5915)
                     /locus_tag="KJL99_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HBD4438069.1"
                     /translation="MFALADINSFYASCEKVFRPDLRNEPVIVLSNNDGCVIARSPEA
                     KALGIRMGQPWFQVRQMRLEKKIHVFSSNYALYHSMSQRVMAVLESLSPAVEPYSIDE
                     MFIDLRGINHCISPEFFGHQLREQVKSWTGLTMGVGIAPTKTLAKSAQWATKQWPQFS
                     GVVALTAENRNRTLKLLGLQPVGEVWGVGRRLTEKLNALGINTALQLAQANTAFIRKN
                     FSVILERTVRELNGESCISLEEAPPAKQQIVCSRSFGERITDKDAMHQAVVQYAERAA
                     EKLRGERQYCRQVTTFVRTSPFAVKEPCYSNAAVEKLPLPTQDSRDIIAAACRALNYV
                     WREGYRYMKAGVMLADFTPSGIAQPGLFDEIQPRKNSEKLMKTLDELNQSGKGKVWFA
                     GRGTAPEWQMKREMLSQCYTTKWRDIPLARLG"
     gene            complement(5915..6352)
                     /gene="umuD"
                     /locus_tag="KJL99_003475"
     CDS             complement(5915..6352)
                     /gene="umuD"
                     /locus_tag="KJL99_003475"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HBD4438070.1"
                     /translation="MSTVYHRPADPSGDDSYVRPLFADRCQAGFPSPATDYAEQELDL
                     NSYCISRPAATFFLRASGESMNQAGVQNGDLLVVDRAEKPQHGDIVIAEIDGEFTVKR
                     LLLRPRPALEPVSDSPEFRTLYPENICIFGVVTHVIHRTRELR"
     gene            complement(6349..6597)
                     /locus_tag="KJL99_003476"
     CDS             complement(6349..6597)
                     /locus_tag="KJL99_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HBD4438071.1"
                     /translation="MIRIEILFDRQSTKNLKSGTLQALQNEIEQRLKPHYPEIWLRID
                     QGSAPSVSVTGARNDKDKERILSLLEEIWQDDSWLPAA"
     gene            6991..7917
                     /locus_tag="KJL99_003477"
     CDS             6991..7917
                     /locus_tag="KJL99_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HBD4438072.1"
                     /translation="MPNWCSNRMYFSGEPAQIAEIKRLASGAVTPFYRRATNEGIQLF
                     LAGSAGLLQTTEDVQFEPCPGLTAAGRGVVSPENIAFTRWLTHLQNGVLLDEQNCLML
                     HELWLQSGTGQRRWEGLPDDVRDTITALFTAKRGDWCGFWSNEDVSVWWNRLCDNVLP
                     EKTMPFDLLTVLPTRLDIEVNGFNGGVLNCVPSAYHWYTERYGVKWPCGYDLNISSQG
                     ENFIQVDFDTPWCQPESDVVAELSRLFGCTLEHWYAEQGCDFCGWQLYERGELVDVLW
                     GELEWSSPTDDDELPEVTGPAWIVDNVAHYGG"
     gene            7914..8225
                     /locus_tag="KJL99_003478"
     CDS             7914..8225
                     /locus_tag="KJL99_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438073.1"
                     /translation="MKYDGDGRATARFFSGKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RWARDGRFYRSRLASVTVYASLSPFSDERPSSRFRGIALPSERRRLRYSTVGLTRYRT
                     R"
     gene            8302..8985
                     /locus_tag="KJL99_003479"
     CDS             8302..8985
                     /locus_tag="KJL99_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HBD4438074.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRSGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWHRIDRFMAAWKRAGFSVAGHLVFTKNYT
                     SKSAYVAYRHECAYILAKGSPARPRNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DNWFEPEAA"
     gene            8986..9207
                     /locus_tag="KJL99_003480"
     CDS             8986..9207
                     /locus_tag="KJL99_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438075.1"
                     /translation="MNYAGHEKLRADVAEVANTMCDLRARLNDMEHRCRFDSDVLVER
                     LTRQTLYRANRLFMEAYTEILELDSCFKD"
     gene            9221..9655
                     /locus_tag="KJL99_003481"
     CDS             9221..9655
                     /locus_tag="KJL99_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
                     /protein_id="HBD4438076.1"
                     /translation="MYGTCETLCRALAAKYPGDTPLMLVIWSPEEIQALADGMDISLS
                     DHEIRTVLARLEDIPEDQRTESGISSGAAMEIINNVRENRQVTVPAELLASLIQTAEQ
                     ALWKREWAARDNGLAVPECVTRRQAVVNQARALLKNNTREND"
     gene            9700..10470
                     /locus_tag="KJL99_003482"
     CDS             9700..10470
                     /locus_tag="KJL99_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438077.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQRNPNHPGWISIYVR
                     LDAPRLATLLINRHGGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLAEDEIRAVLAAVRDAIRSICYQVADDARRIRAALTTTGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAIVNRGARFSAVEMYLVSECVEHILSSGLACDVLR
                     IPDEPPRRWFDRDVLREVVREARAEIRSMADALAKIRG"
     gene            10882..11307
                     /locus_tag="KJL99_003483"
     CDS             10882..11307
                     /locus_tag="KJL99_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HBD4438078.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAQKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTQLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            11354..11776
                     /locus_tag="KJL99_003484"
     CDS             11354..11776
                     /locus_tag="KJL99_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HBD4438079.1"
                     /translation="MYCTVKEIIREVLDTDVPDSECVFAVVLTRGDVRHIAQDWSLTD
                     DELETVMQRLDDAFEHGADVSVVHDVVRELMEEKRASRQVTVPAVMLEKVLALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGEHMS"
BASE COUNT         2702 a         2992 c         3264 g         2929 t
ORIGIN      
        1 ttatcatatc atttgcgtct tttcttccgc gcagatccgg tttccggcgg ttcaggatct
       61 tcctctcccc ccacacaacg ccacctcctg tcagcacaac atgtggtatc tcattcagct
      121 gcttatgaca ctatatgttg tgtcatctcc ctgacctgtg atgcgccgcg caggggcgga
      181 aaacagcgat atgatgattt tctcagcgtg gtacacttcc ggaaagttgt gatattccgg
      241 aaagtcggat ctgacggaaa cggctctccg gtaatttaac ggcgtggtta tatggatgct
      301 tgttatcatg gtgatgatga taacggcatg atgttatctg acggcgtgac ggtaagggca
      361 gtgatgatgg atgacgttat cgcatgaccg tccctgcccg gaaaagaaaa aaggagtcac
      421 ccatgttttt tattgagaat gaaggtcagg ctgtcgccgg aacggattac tggcagtctg
      481 tacaggcgca ggccggatat gtctacctca gctggaatgc cggcgcagcc aggctgcttg
      541 tcccggatgc ggcaaaacat ttactcaggg agatgcgggg ggctgagtac gtcatcatca
      601 gtaagggagc actgcatggc cgcgatgcgc tggaactggt atttgaagac ggcagcgatg
      661 cgccgtttgt gatccacatg ctgagtgagc agtgcgatcg cctgctcccc gaaaacaacc
      721 agggaggggg ttttgttgtc accgtctgga cgcgtggcgg taaccagctc cgttatccgg
      781 gaaagtaccg tgttgtggaa aacctgcccg acgtttcccc gtggagtgaa cactgatatg
      841 cagcacctgc cggcaccgat ccaccatgcc cgggatgccg ctcagcttcc tgttgccatc
      901 gattatccgg cagcgctggc actccgccag atgtcgatgg ttcatgatga actgcccaaa
      961 tacctgctgg cccctgaagt gagcgccctg ctccattacg tcccggatct gcgccgcaag
     1021 atgctgctgg ccacactgtg gaacaccggt gcgcgcatta atgaagcact ggcgctgacg
     1081 cggggggatt tttcgctcac gcccccgtat ccgtttgtgc agcttgcgac cctgaaacaa
     1141 cggacggaaa aagccgccag gacggcagga agaatgcccg ccggtcagca gactcaccgg
     1201 ctggttccgc tctccgacgc ctggtacgtc agccagctgc agacgatggt ggccacactg
     1261 aaaatcccca tggaacggcg taataaacga acaggcagga cagagaaagc gcggatctgg
     1321 gaagtgacgg acagaacggt caggacctgg attggggagg cggttgccgc cgctgccgct
     1381 gacggtgtga cgttctctgt cccggtcacg ccacatacgt tccgccattc ctatgcgatg
     1441 catatgctgt atgccggtat accgctgaag gtcctgcaga gcctgatggg gcataagtcc
     1501 atcagctcaa cggaggtcta cacgaaggtg tttgcactgg atgtggctgc acggcaccgg
     1561 gtgcagtttt cgatgcctga gtccgatgcg gtgtctatgc tcaaaagaat tccataagct
     1621 ctgacttttt taaatccctt tcggatatct catggatata ttatcggtat ttcatattct
     1681 gttgacatgc aggaccggta tagagtaaaa aaacatctat tgagtacctc ttggatatcc
     1741 gaaaggtgtc ttatggggtc tttatgggta ttacatgtgg attctggaga tacactatgc
     1801 ctgttattgc taatacgcat cccaaaggtg gtgtgggtaa gacaacttca tccgtaaaca
     1861 ttgttggtga aatgaagtct gatacagttg atctggatac tcataccggg ctttctatca
     1921 ttctggggct gagacctgaa ggaaaagaaa tttccgtgaa agtacccaaa acagtggatg
     1981 agttaatcga aattatgact ccctacaaga acagcgataa gacgttactt attgactgtg
     2041 ggggatttga ctcagatctt acccgtactg cgattgcctt tgcagactgc gttattgtcc
     2101 cttcaaaaga ctccctgact gaacgtattg gtttgatgca ttttgatgga gtactggacg
     2161 aaatcagctc tatcatgggg accgatataa ctgctcacct gtatctctgt aaagttaatc
     2221 ctaacaagaa gaaattccct aagcttgatg caatcctgcc atcgttcaaa catctgaagc
     2281 tgatgaagag ccgcatttct gctcgtgcag aatttgagga tgtcattgag actgggatgg
     2341 gaatcactga gtctgttcat gggcgctatt cggcaggagg taaggaagtt attgccctga
     2401 tcgaagaaat caatcatctg attgaaaata acaaacagta aggtgtgtct attgggtatc
     2461 catatagtat cctttgggct tttaatggat atcttttaag tatctaacaa acatcctggg
     2521 ttatctcatg agcaaagcga aatttagtgc acaggctgaa aaactgaaaa acctggtgga
     2581 ttctgccaga ttacaaactt cttcttctgc cgtaacagca acagccccgt ctcagccaaa
     2641 tgaaggtgca gaggaagttt caggtcgtcc cgtggctgcc aggacgaagg ctaagaatct
     2701 taaggccata ccgctgtcgt attttgaagc tcatgcggaa cttaaagcga ccagtaaaac
     2761 atctctggat tttagctcat atattattga ggctataaga gagaagcttg aacgtgatgg
     2821 tgcaataggt caataaacta tcggatacct taagggtatc tgatgtgtgt cttttaaatg
     2881 cgttatgggg attcttcacg tacctaaaga gtccccatta gacatctttc tgtttatggt
     2941 gacatgattc gtcaccgctt tcctttcctc tcagtttcac catttcattc cttttccctt
     3001 aacctgaacc atgatgtcca cgatgttttt atattgcatt tatgtgtcac atataacctt
     3061 tataatgttt ttgtatctat gatttcctct ggtcttaaag ttgtgtgtac aatgtacata
     3121 caatttatcg tgtttttatg ttgcatttgt cctttcagaa agactgtttt ttcattataa
     3181 ttgaatatac attgcatgta tgaagtatgt ttgttttttc tatttgttta tgagcaacgg
     3241 agactggtta tgaaaatctt tattgatgat ggttctacca acattaaact tgcctggttg
     3301 gaggatggtg acgtgaaaac gttaatcagc cctaacagct ttaaaccgga atggtctttt
     3361 agtttactgg atgatgctgc tccggctaat tacgagatcg acggcgagaa gttttcattt
     3421 gatccattaa gtgctgatgc tgtcgtaacg actgaaacac gctatcagta cagtgatgtg
     3481 aatgttgtgg ccattcagca tgcgctacag cagaccggcc tgaaagcgca gcctgttgat
     3541 gtcatcgtca cattgccgat ctctgaatac cttgacgcca acaatcagaa aaataagcaa
     3601 aacattgaaa gaaagaaaaa aaatgtgatg cgtgaagtac gggtgcaggg gagtgatgca
     3661 tttgttattc gcagtgtttc cgttcttcct gaaagtattc ccgctggttt cagtgttctt
     3721 gcggggcttg aggatgatga atcccttttg atcgtagatc tcggtggtac aactcttgat
     3781 gtgtcgcatg ttcgtagcaa aatgacaggg attacaaaaa cctggtgtga tccgaatatc
     3841 ggcgtgtccc tgattacatc cggggttaag gaacagatgg ctgtacatgc taatacaaga
     3901 gtgagctcat tccaggctga caatatcatt gtgcaccgta atgaacctga ctacctttcc
     3961 cgtcgcattt ataatgctga acagcgtgag tctatcatta atgttatcaa tgaacggcag
     4021 aagttgctta ttaaacgagt taatgatgtc atcagccgtt tcaccgacta tacacatgta
     4081 atgtgtgttg gtggtggggc tgaaattgtg gccgaggctg tgaagaacct gactaaggtt
     4141 cctgatgagc gcttttatct gagctcatcc ccgcagtttg atttagtcat gggaatgata
     4201 aaaatgaaag gtggtgtgac taatgagtga tgaaaataaa tccagacgct gctcatttga
     4261 acttttcccg gatgaacgga caggagataa gatagcagat gagctcattg caaatgaaaa
     4321 actgaaggag cggggacgtt tcatgcgcgc gatgttagtt actggtgcgg cgtttgcagc
     4381 tatagataaa cgtcttcctt tgctgatatc ggagttactg acagaaaata cgactctgga
     4441 tgatattaat aaggtgattt ccagcgtaat accaggtgct ttttcggttg aaaagaaatt
     4501 gcttgaatta ctggaaaaac aatctggtct ccatacgtct gtggattgca gtactcctct
     4561 tacagagcag agtctgtcga gaaatgacgg tgaagaccag accagaagaa acgctgaaaa
     4621 tatgttcgga gatgactgaa ctaacccagc ctggccaggg gaatatctcg ccatttagtt
     4681 gtataacact gactcaacat ttcccgtttc atttgccatt caggggcggt tcctcgcccc
     4741 gcaaaccaca ctttcccttt tcccgactgg ttcagttcat cgagtgtttt cattaacttt
     4801 tcactgtttt tacggggctg gatttcatca aataatcccg gctgcgctat acccgatggt
     4861 gtgaaatcag ccagcatgac tcctgccttc atatagcggt acccttcacg ccagacatag
     4921 tttaaggctc tgcatgcggc ggcaataatg tcccggctgt cctgtgtggg caatggaagc
     4981 ttttccacag cggcattgct gtaacagggt tcttttactg caaaggggga tgtccgtaca
     5041 aatgtcgtca cctgccggca atactgacgc tccccacgta gtttctctgc ggcccgctct
     5101 gcatactgaa caacagcctg gtgcatggca tctttgtctg tgattcgttc accaaaactg
     5161 cgactacaga caatctgctg ttttgccggt ggtgcttctt ccagggatat gcaggactcg
     5221 ccgttgagtt cgcgtaccgt acgctcaaga atgacgctga agtttttccg gatgaatgcc
     5281 gtgttagcct gcgccagctg cagtgctgtg ttaataccca gcgcattcag cttttccgtc
     5341 agtctgcgtc ctactcccca gacctcacca actggctgca gtcccagtag cttcaaggtc
     5401 cgattacggt tttctgccgt cagcgcgacc actccggaaa actgtggcca ttgctttgtt
     5461 gcccactgtg cacttttagc cagcgttttt gtaggcgcaa tgcccacccc catggtgagt
     5521 cctgtccagc tttttacctg ttccctgagc tgatgaccaa aaaactccgg agagatgcaa
     5581 tgatttatcc cccgcaaatc aataaacatt tcatcaattg agtagggctc aactgcggga
     5641 gaaagcgact ccagaacagc catgacccgt tggctcatgc tgtggtacag cgcataattg
     5701 ctggaaaata catgtatttt cttctccagg cgcatctgtc tcacctgaaa ccagggctgc
     5761 cccattctga tgccaagggc ttttgcctcc gggctgcgcg cgatcacaca gccatcgtta
     5821 ttgctgagta cgatgaccgg ttcgttgcga aggtccgggc ggaaaacttt ttcacatgag
     5881 gcgtagaaac tgttgatatc agccagtgca aacattagcg taactcccgc gtcctgtgta
     5941 tcacgtgagt gacaacacca aaaatacaga tgttttccgg atacagtgtg cggaactccg
     6001 ggctgtctga aaccggctcc agtgccgggc gtgggcgcaa cagcagtcgt ttgacggtga
     6061 actcaccgtc gatctcagcg ataacgatgt ccccgtgttg tggtttttcg gccctgtcca
     6121 ctaccagcag atcaccattc tgcacgccag cctggttcat cgattcaccg ctggcgcgca
     6181 gaaagaaggt ggctgcaggt ctgctgatgc aatagctgtt cagatccagt tcctgctcag
     6241 cataatcagt ggcaggcgaa ggaaaaccgg cctggcaacg atcggcaaac aacggtcgca
     6301 cgtaactgtc atcgcctgaa gggtcagccg gacggtgata aacggtactc atgctgcagg
     6361 cagccagctg tcgtcctgcc agatttcttc cagcagagac aggatacgtt ctttatcctt
     6421 gtcgttgcgg gctcctgtga cagatacaga cggggcgctg ccctggtcta tacgcagcca
     6481 gatttccggg tagtgtggtt tcagacgttg ttcgatttca ttctgtagcg cctgaagtgt
     6541 gcctgatttg aggttttttg tgctctggcg gtcgaaaaga atttcaatgc ggatcatgtt
     6601 ctcatctccc ttttctattg ctgtatgcat atacagtatt ctaataactg aatccacaca
     6661 cagtcaaatg tcattattgt gactaaaaaa cgctcaactc tctgagagtg tggctgtatg
     6721 cctgctgtgg actgctgtgt ccagcttatc cacagcattt tgcgcacggt tctgtgaata
     6781 aaatacctgg ttacccaggc cgtgccggca cgttaaccgg gctgcatccg atgcaagtgt
     6841 gtcgctgtcg ccggcctcct cacccggtca cgtttcgtcg ttcctcctcc acgcgctgcg
     6901 gcttcggggc cgcacctgca ttcgtatgcg gtcgcccggt tacaggtgcg gcacggcctg
     6961 atggaggccg catgtgagag gagaattccc atgccaaact ggtgctcgaa tcgtatgtat
     7021 ttttctggtg aaccggcaca gatcgctgag attaaacgac tggccagcgg cgcagtcaca
     7081 ccgttttatc gccgcgccac aaatgaaggt attcagctgt ttctggccgg aagtgccgga
     7141 cttctgcaga ccactgaaga tgtgcagttt gaaccgtgcc ccggactgac ggctgccgga
     7201 cgtggtgttg tatcgccgga gaatatcgcg ttcacacgct ggctgacaca cctgcagaac
     7261 ggtgtgctac tggatgaaca aaactgcctg atgctgcatg aactctggct gcagagcggt
     7321 actggacagc gtcgctggga aggattaccg gacgatgtca gggataccat caccgcactt
     7381 ttcaccgcaa aaagaggtga ctggtgtggc ttctggagta acgaggatgt atcggtgtgg
     7441 tggaaccgtc tgtgtgacaa cgtactgccg gaaaaaacca tgccgtttga cctgctgacg
     7501 gttttgccga cccgcctgga tattgaagtg aatggcttta acggcggtgt tctgaactgt
     7561 gttccttctg catatcactg gtatacagaa cggtatggcg tgaagtggcc ttgtgggtat
     7621 gacctgaata tcagcagtca gggggaaaac ttcattcagg tagatttcga cacgccgtgg
     7681 tgtcagccgg aaagcgacgt tgttgcagaa ttaagccgcc ttttcggctg cacgctggag
     7741 cactggtacg ccgaacaggg ctgtgatttc tgtggctggc agttgtatga gcgcggagag
     7801 cttgttgatg tgctgtgggg ggaactggaa tggtcttccc cgacagatga cgatgagctg
     7861 ccggaagtca ccgggcctgc gtggatagtc gacaatgtgg cgcattatgg cggatgaagt
     7921 atgacggaga cgggcgggca accgcccgtt tcttttccgg taaaggatgt cgccgtgctc
     7981 ctctttttac tgcgccggct gacgcggcgc ggcataaacg ctgcctgtgg tcagtgtccc
     8041 gcgtccggtg ggcacgggac gggcgctttt accgctcccg gctggcgtcg gtgacagtgt
     8101 acgccagcct gtcgcccttt tctgatgaac gcccttcaag ccgctttcgc ggcatagcct
     8161 tgccgtcaga aagacgacgg ctgcggtatt ccacggtcgg cctgacccgt taccggacgc
     8221 ggtgaacagc ccacaggcag cggggaacgg gcaccacagg ggtgccctcc cggtgtcctc
     8281 tgtaagagaa ggagcgttgt tatgtcccgt tttgtcctcg gtaactgcat cgatgttatg
     8341 gcccgtattc ctgataacgc cattgatttc atactgactg acccgccgta cctcgtcggt
     8401 tttcgtgacc gttccgggcg caccatcgct ggcgataaaa ccgatgaatg gctgcaaccg
     8461 gcctgtaatg aaatgtaccg cgtactcaaa aaagacgcgc tgatggtcag cttctacggc
     8521 tggcaccgta ttgaccgctt tatggcggcc tggaaacgcg ccggattcag cgtggcaggg
     8581 cacctcgtgt tcaccaaaaa ctacacatcc aaatctgcgt atgtcgcgta ccgccacgaa
     8641 tgcgcttaca tcctggcaaa gggcagtccg gcccggcccc gtaaccctct gccggatgtg
     8701 ctgggctgga aatattcagg caatcgtcat cacccgacgg aaaagcccgt taccagcctg
     8761 caaccactga ttgagagctt cacacatccg aacgcgattg tgctggaccc gtttgcaggc
     8821 agcggctcaa cctgcgtcgc cgccctccag tccggacgcc ggtatatcgg tatcgagctg
     8881 cttgaacagt atcaccgtgc cggacagcaa cgactggccg ccgtgcaacg ggccatgcag
     8941 cagggggccg cgaatgataa ctggtttgaa ccggaggctg cgtaaatgaa ctatgccgga
     9001 cacgaaaaac ttcgcgccga cgtggcggag gtggccaaca ccatgtgtga ccttcgggcc
     9061 aggctgaacg atatggagca tcgctgtcgc tttgattccg atgtgcttgt tgaacgcctg
     9121 acacgccaga cgctgtatcg cgccaatcgc ctgttcatgg aggcgtatac cgaaattctg
     9181 gaactggatt cctgcttcaa agattgagaa ggagacgaga atgtacggaa catgcgaaac
     9241 gctctgccgg gcgctggcag caaagtatcc gggagatacg ccgctgatgc tggttatctg
     9301 gtccccggaa gaaattcagg ccctcgctga cggaatggat atttccctgt ccgatcatga
     9361 aatcaggact gtcctggcgc gcctggagga catcccggaa gaccagcgga ctgaatccgg
     9421 tatttcttcc ggtgcggcga tggagatcat caataacgtg agagaaaacc gccaggtgac
     9481 cgtccctgct gaactgctgg cgtccctgat tcagaccgcc gaacaggcac tgtggaaacg
     9541 tgagtgggcc gcccgtgata acggcctcgc cgtcccggaa tgcgtcaccc gccgacaggc
     9601 ggtggttaat caggcccgcg ccctgctgaa aaacaacaca cgcgaaaacg actgatgtta
     9661 tcgccgcctg cgggcggcga ttcagggaga ccatgaatca tgaacgaaac gctgaatgca
     9721 ctgatttgtc gccacgcccg taacctgctg ctggcgcagg gctggccgga agagacggat
     9781 gttgaccagc gaaatccgaa ccatccgggc tggatcagca tttacgtgcg gctggatgcg
     9841 ccccggctgg cgacgttact tatcaaccgt cacggcggcg tactgccgcc gcacctggcc
     9901 tctgccattc agaaactgac cggaaccggg gcggaacttg ttctgtccgg cagtcagtgg
     9961 cagtcgctgc cggtacttcc ggcagacgga acgcaggtgt ctttcccgta tgccggtgag
    10021 tggctggcag aagacgaaat cagggctgta cttgcggcgg tgcgcgatgc aatacgaagc
    10081 atctgttacc aggtggcaga cgatgcgcgg cgtatccggg cggcgctgac caccaccggt
    10141 cagacgttgc tgacccgcca gacgcgccgc tttcgtctgg ttgtgaagga aagcgatcac
    10201 ccctgctggc tcgatgaaga cgacgaaaat ctgcccgtgg tgctcgatgc catcgtgaac
    10261 cggggagcac gtttttcggc agtggaaatg tacctggtca gtgagtgtgt ggagcatatc
    10321 ctgtccagtg ggctggcctg cgatgtgctg cgtataccgg atgaaccgcc ccgccgctgg
    10381 tttgaccgtg atgttctgcg ggaggtggtc cgggaggccc gggccgaaat ccgcagcatg
    10441 gcggatgccc tcgcaaaaat ccggggctga tgatggcggg gaaaatcccc gccttttcct
    10501 gccagtcccc ggacagagca caaaggaagt cgccgccggc tccgctttac ccggccatgc
    10561 ggtgcatggc cttgtgggtt ttcagttatg tggccgccgt gtcgtgtgcg ggctgtgccg
    10621 tgcctccatc ttagccgggc tggcggggat gcaagggtaa aatgaacgcg gcggtcaccc
    10681 ggtccctcct tcccgtctgc cgtgattttc cggtcgttca cccgctcaga tttcgcggtc
    10741 tcaccctgca actcccgtca gccgtgtgcg tcaggctgcg gcttcccttg cgcccctgca
    10801 tccccgcctg ttcgcccggc ttttatggag gcacggcacc gcccggtgtc gcggaatatg
    10861 tgaacaacgg aggaaaacat catgcaatat gcgaaacctg tcactctgaa cgttgaagag
    10921 tgcgaccgtc tctcctttct gccgtatctg tttggcaacg attttctgta tgccgaggcg
    10981 tatgtgtacg cgctggcgca aaaaatgatg ccggaatatc agggcggctt ctggcacttc
    11041 atccgcctgc cggacggtgg cggttacatg atgccggacg gtgaccgttt ccacatggtg
    11101 aacggtgcaa actggtttga ccgtaccgtg agtgctgatg ccgcaggcat catccttacc
    11161 tcccttgtga ttaaccgcca gttgtggctg taccacgaca gcggggatgc aggactgacc
    11221 cagctttacc ggatgcgcga tgcgcagctg tggcgtcaca tcgaatttca ccctgaatgc
    11281 aacgcgattt acgcggctct ggactgatta acggacgggg cggcaatgcc gcccctgtaa
    11341 aggagacgag aacatgtact gtactgttaa agaaattatc cgcgaagtgc tggatacaga
    11401 cgtgccggac agtgaatgcg tttttgccgt ggtgctgacc cgtggggatg tgcgtcacat
    11461 agcccaggac tggagtctga cagacgatga gctggaaacc gtcatgcagc ggctggacga
    11521 tgcctttgag catggtgcgg atgtcagcgt tgttcacgac gttgttcgtg aactgatgga
    11581 agaaaagcgc gccagccgtc aggtgacagt cccggcggtg atgctggaaa aagtgttggc
    11641 gctggcaggc agtgaaatga agcgcctgta tgccgtcggg agtgagaacg ggggcgacgg
    11701 tgatgcgttc gtcagggaag aacgcgaagc aatggacgtt gtgttacagg cgctggacgg
    11761 ggagcacatg tcatgaatat cagcacagaa acccgcgaaa ttctgcgcaa ttacaaagcc
    11821 gtgattaatg cgcggcgtcg tgaaatgggg cagaaaccgc tcaccactgc gcagattgtt
    11881 gatgaaa
//
LOCUS       DAEBXN010000122        11684 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.122, whole genome shotgun sequence.
ACCESSION   DAEBXN010000122 DAEBXN010000000
VERSION     DAEBXN010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11684
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.122"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..348
                     /locus_tag="KJL99_003485"
     CDS             <1..348
                     /locus_tag="KJL99_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="catalyzes the formation of 3-methylcrotonyl-CoA
                     from isovaleryl-CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HBD4438080.1"
                     /translation="SGNIMCLDVLRVLNKQAGVYDLLSEAFVEVKGQDRYFDRAVRRL
                     QQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQAWCQVMLDTRGGVRLSEQIQ
                     NDLLLRATGGVCV"
     gene            complement(465..740)
                     /gene="yjfN"
                     /locus_tag="KJL99_003486"
     CDS             complement(465..740)
                     /gene="yjfN"
                     /locus_tag="KJL99_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HBD4438081.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(889..1218)
                     /gene="bsmA"
                     /locus_tag="KJL99_003487"
     CDS             complement(889..1218)
                     /gene="bsmA"
                     /locus_tag="KJL99_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HBD4438082.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            1400..2149
                     /gene="yjfP"
                     /locus_tag="KJL99_003488"
     CDS             1400..2149
                     /gene="yjfP"
                     /locus_tag="KJL99_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBD4438083.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNKIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(2146..2901)
                     /gene="ulaR"
                     /locus_tag="KJL99_003489"
     CDS             complement(2146..2901)
                     /gene="ulaR"
                     /locus_tag="KJL99_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HBD4438084.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLG
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(3009..4073)
                     /gene="ulaG"
                     /locus_tag="KJL99_003490"
     CDS             complement(3009..4073)
                     /gene="ulaG"
                     /locus_tag="KJL99_003490"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HBD4438085.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEIKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            4428..5825
                     /gene="ulaA"
                     /locus_tag="KJL99_003491"
     CDS             4428..5825
                     /gene="ulaA"
                     /locus_tag="KJL99_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBD4438086.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASLIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWVVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            5841..6146
                     /gene="ulaB"
                     /locus_tag="KJL99_003492"
     CDS             5841..6146
                     /gene="ulaB"
                     /locus_tag="KJL99_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HBD4438087.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            6156..6620
                     /gene="ulaC"
                     /locus_tag="KJL99_003493"
     CDS             6156..6620
                     /gene="ulaC"
                     /locus_tag="KJL99_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HBD4438088.1"
                     /translation="MKLHDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            6634..7284
                     /gene="ulaD"
                     /locus_tag="KJL99_003494"
     CDS             6634..7284
                     /gene="ulaD"
                     /locus_tag="KJL99_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HBD4438089.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            7294..8148
                     /gene="ulaE"
                     /locus_tag="KJL99_003495"
     CDS             7294..8148
                     /gene="ulaE"
                     /locus_tag="KJL99_003495"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HBD4438090.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETN
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKACDWVKARMA
                     KAGMVEAA"
     gene            8148..8834
                     /gene="ulaF"
                     /locus_tag="KJL99_003496"
     CDS             8148..8834
                     /gene="ulaF"
                     /locus_tag="KJL99_003496"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HBD4438091.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(8963..9238)
                     /locus_tag="KJL99_003497"
     CDS             complement(8963..9238)
                     /locus_tag="KJL99_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBD4438092.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            9565..9960
                     /gene="rpsF"
                     /locus_tag="KJL99_003498"
     CDS             9565..9960
                     /gene="rpsF"
                     /locus_tag="KJL99_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HBD4438093.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            9967..10281
                     /gene="priB"
                     /locus_tag="KJL99_003499"
     CDS             9967..10281
                     /gene="priB"
                     /locus_tag="KJL99_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HBD4438094.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            10286..10513
                     /gene="rpsR"
                     /locus_tag="KJL99_003500"
     CDS             10286..10513
                     /gene="rpsR"
                     /locus_tag="KJL99_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HBD4438095.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            10555..11004
                     /gene="rplI"
                     /locus_tag="KJL99_003501"
     CDS             10555..11004
                     /gene="rplI"
                     /locus_tag="KJL99_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HBD4438096.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(11075..11638)
                     /locus_tag="KJL99_003502"
                     /pseudo
     CDS             complement(11075..11638)
                     /locus_tag="KJL99_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
BASE COUNT         2767 a         2873 c         3087 g         2957 t
ORIGIN      
        1 tccggcaata ttatgtgcct ggatgtgttg cgcgttctca ataagcaagc gggcgtatac
       61 gacttattgt cggaagcatt tgtggaagtg aaagggcagg atcgctattt tgatcgcgcg
      121 gttcgtcgtt tacagcagca gctgcgtaag ccagctgaag aactggggcg agagattact
      181 catcagctat tcctgctggg ctgcggtgcg caaatgttga aatatgcttc tccgccaatg
      241 gcgcaggcgt ggtgtcaggt gatgttagat acgcgcggcg gcgtacggtt gtcagagcag
      301 atccagaatg atttattgct gcgggcgacg gggggagtgt gtgtgtaagc gtatacgact
      361 gatgcgacgc tggtttcgat taactaaatg aaatatgtga aaattgtagg ccggacaagg
      421 cgctcgcgcc gcatccggca ttgttcattt gctgtagatg acactcatgc atacagtatc
      481 gcctgtacgc gccagttatc aggctgctgg tcttcgtaca tggtaataat gcggtaccat
      541 gacgcacctt gttcatcggc ctttaacgcc acgatacgtt ccacgtcctg cggatcccct
      601 gaaatattgc taactgagat ctgacctatt tcattcaggc ccgtcacgca gtcagcactg
      661 gcgaattctg cagactgtgc cgtggcgctc gcaggataag tcactaattg cagcgagggt
      721 gaggcaagta attgtttcat cgtcagctcc atttttcgtt tccccacatg ttgcagggca
      781 ttccatcgcg gtaataaacg cccaagcttc cagccggtaa tttattgtgc acaggtaaac
      841 gcatagggac gcaaagcaat gtaaagctga ctatgtaagt tgtctgattt atttacggta
      901 caaaatggct tgtgaatacc actgtcctgt cacgatggtt tcgtccacca tgacgacaac
      961 gtaataatca gcttttgcag cgacagcttt tgctctgatt tctgctaatg cgtcatccgg
     1021 agaaccccga accatcgtgc ttacgctacc tattcgctgt aacccttgcg tctggtcgcg
     1081 gcgaatctct tgcggatggt ccgttactgg cggtgctggc tgtggcgtac cttgcagtgc
     1141 gctacaggca cttaacatca acaccaataa taaactggca aaccggtaaa taacgctatt
     1201 acgtttcctg ctaaccatag tgtagtgcct tattaattta actttggggg aatgttcccg
     1261 aattgttacc tggtacgcga ttttcgaatg aaaacgtgac gaaagcgtaa cccacatctc
     1321 aacattatga actaataccg ccagcagaaa cttgtgctaa ggatttcttc atgtcatacc
     1381 agacaaaaag gagagacaga tgattgaaat agaatcacgc gagctggcag atattcccgt
     1441 tcttcatgct tatcctgtcg ggcaaaaaga taccccgtta ccgtgcgtaa ttttttatca
     1501 cggctttact tcatccagcc tggtgtatag ctattttgcc gttgcgctgg cgcaggctgg
     1561 tttgcgggtg atcatgccgg atgcgcccga tcacggtagc cgttttagtg gcgacgaagc
     1621 gcggcggtta aatcaattct ggcaaatctt gctacaaagt atgcaggaat tcactacttt
     1681 acgtgcggca atagccgaag aaaactggct gcttgatgac cgtctggcag tcggtggcgc
     1741 gtcgatgggc gcgatgacgg cactgggaat taccgctcgc caccccacgg tgaaatgtac
     1801 cgccagcatg atgggatcgg gctattttac atcgctcgcc cgttcactgt ttccaccgct
     1861 gatacctgaa acggcagcac agcagaatga attcaataaa attgtcgcgc cgctggcaga
     1921 gtgggaagcg acaaaccacc tggaacaact tggggacaga cctctactgc tgtggcatgg
     1981 cctcgacgat gacgttgtgc ctgccgacga atcactacgt ttgcagcagg ccttaagcga
     2041 gacgggacgg gataaactgc taacctgttc atggcagcca ggcgtgcgtc accgcattac
     2101 gcctgaggcg ttagatgctg ccgtgacatt tttccgccag catctttaaa cacgcagaat
     2161 gctgacccct tgcgcttcca gttgttgcag gatttccggg ttagcatttt tgccggtgat
     2221 cagcatatcg atttgatcgg cacggctaaa aagcattccc gcgcgttcgc caatcttact
     2281 gctatcaacc agtaccacca gtttccctac cacgctcagc atcttctgct ctgccattgc
     2341 tgtcagcata tcggttttat acaacccttc tgcggtcagc ccttttccgc tggtaaacat
     2401 ccagtgcccg gcatagagac tgttttcgct gccctgcggg cttaaagtga tggactgact
     2461 tttgttgtac tgtccaccca taatgatcac gctgtcatgt tcttgatcga tcaggtaatt
     2521 tgccagcggt agataattag tgatgatttg cactggcttg ccacacattt cccgcccaag
     2581 cagaaacgcg gtggagccgc agttgatgac tacgctttcg cccggattaa ccagctgcga
     2641 ggccgcttta gcaatacgta ctttttcatc gtgattctgc gcctgatgca gattcatcgg
     2701 cgtccagcgc gggcgctgtt gggtaatagc ttctgcgcca ttgcgcacct ttttcagttt
     2761 gccgctttcg ccaagtttat tgatatcgcg tcgcgcagtg gcaggcgaaa ttcccagacg
     2821 ctcaacgact ttctcaacgg tcacaaagcc caattgtgcg agcatttcca ggaggatttg
     2881 atgtctttgt gcttcagtca tgagctattc cgataaaatt tgattttttt agatgatatt
     2941 tgaaatagcc aggaaatact acgccggata gcgcgaactt ctccacgcta tccggtataa
     3001 acgacagatt acaggaatga cttgaacggc agatccggtt caatagtgaa gcaatcatcg
     3061 aaaccgcgcg gatagtggta ctcgaagttg tctttatcca gcggccaggt aaatttgccg
     3121 ccaacctgcc agataaacgg cttgaagcca tacttcagac gatctttttt gatctcccac
     3181 agcacgcgga tctcttgcgg atcggcctgg aagtttgacc agatgtcgtg gtggaacggg
     3241 ataaccactt tcgcattcag ggcttcaccc atacgcagca tatcggcgct ggtcattttg
     3301 tcggtaatac cgcgcgggtt ctcaccatac gatcctaacg ccacatcgat ctgatgttcg
     3361 ttaccgtgct tcgcataata gttagagtag tgggaatcgc cactgtgata tagggaaccg
     3421 ccaggggttt tgaacaggta gtttactgcg cgatcgtcca tgccatctgg cagtacgcca
     3481 gccgcttttt gatcggcagg cagggtgatc agtgcagtac ggtcgaaagc gtcaagcgca
     3541 tgaatttcaa tgtctttcac ttttacaaca tcgcccggtt tgaccacgat gcaacgctct
     3601 ttcggtacgc cccagccaat ccacaaatca acgcaggttt tcggtccaat gaatggtacg
     3661 tcatcagcac agttctgcat cacggcagca gcgacgttaa cgtcgatatg atcgttgtga
     3721 tcgtgagtcg ccagtaccgc gtcgatctgg cgaatcgcaa acggatcaag aacaaacggg
     3781 gtggtgcgta ggtttggctg cagttttttc acgccagcca tgcgctgcat ctggtgaccc
     3841 tgtttcatta acgggttacc gtgactttgt ttgccagtgc cgcaccagaa atcaacgcaa
     3901 acgttggtgc caccttctga tttcaaccag atcccggtgc agcctagcca ccacatcgca
     3961 aatgtgccag gggcgacctg ctcttgttca atttcttcat tcagccagct accccactcc
     4021 gggaaagtgc tcaggatcca ggattctcgg gtgatacttt tcactttact catcgccatt
     4081 taccttcatg atagttcaat tcgaatcaac atgtgattgg ttttgattaa tcctgacact
     4141 attccttcag gaaggtaatg accatttttt gactttttgc cagggaagtt gttgttgatt
     4201 ttgagtgggg aaagatttaa tggaatgtgt agttaaatta actaaatgaa tttaaataaa
     4261 taattgtttc gttgtgtgaa tcccactcta tccatgtgga attatttgcg ggtcgcgtca
     4321 catttaatca taaataatct tgttgtgatt acttttgaaa attagagtga gtgcacaaca
     4381 ttccgggtgt gcggaatacc cggttacctc ttcttcagga gatcgttatg gagatcctct
     4441 acaacatctt taccgtgttt tttaaccagg tcatgaccaa tgccccgttg ttgctgggta
     4501 ttgtgacctg tctgggctac atcctactgc gcaaaagtgt cagcgttatt attaaaggca
     4561 cgattaaaac cataattggt ttcatgttgt tgcaggcagg gtccggcatc ctcaccagca
     4621 ccttcaaacc ggtggtggcg aaaatgtccg aagtctacgg cattaacggc gcaatttccg
     4681 atacttacgc gtcgatgatg gcaaccatcg accgcatggg cgatgcctat agctgggtag
     4741 gttacgcggt gttgttagcg ctggcgctga acatctgtta cgtgctgctg cgtcgcatta
     4801 ccggtattcg taccatcatg ctgactggtc atatcatgtt ccagcaggcg gggctgattg
     4861 ccgttacgtt gtttattttc ggctactcca tgtggacaac cattatctgc acggcgattc
     4921 tggtttcgct ctactggggc attacctcca acatgatgta caagccgact caggaagtga
     4981 cggacggctg cggtttctcc atcggtcacc agcaacagtt tgcatcatta attgcctata
     5041 aagttgcgcc gttcctcggc aaaaaagagg aaagcgttga agacctcaaa ctgccaggct
     5101 ggctgaatat tttccacgac aacatcgtct ccacggcgat tgtcatgacc atcttctttg
     5161 gtgccattct gctctccttc ggtatcgaca ccgtgcaggc gatggcaggc aaagtgcact
     5221 ggacggtcta tatcctgcaa accggttttt ccttcgcggt ggcgatcttc atcatcactc
     5281 agggtgtgcg catgtttgtg gcggaactct ctgaagcgtt taacggtatc tcccagcgcc
     5341 tgatccctgg tgcggttctg gcgattgact gtgcggctat ttatagcttc gctccgaacg
     5401 ccgtggtctg gggctttatg tggggcacca tcggtcagct gattgcggtt ggcatcctgg
     5461 tcgcctgcgg ttcctcgatc ctgattatcc caggctttat cccgatgttc ttctccaacg
     5521 ccaccatcgg cgtgttcgct aaccacttcg gcggctggcg tgcggcgctg aagatttgtc
     5581 tggtgatggg gatgatcgaa atctttggtt gcgtctgggt ggtgaaactt accggtatga
     5641 gtgcctggat ggggatggcg gactggtcga ttctggcacc gccgatgatg cagggcttct
     5701 tctccatcgg tatcgccttt atggccgtca tcattgtaat tgcactggct tatatgttct
     5761 tcgctggccg cgcactgcgc gcagaagaag atgcagaaaa acaattggca gaacagtctg
     5821 cttaataagg agttttgatt atgaccgtac gtattctggc tgtgtgtggc aacggacaag
     5881 gcagttccat gatcatgaag atgaaagtgg accagttttt aacccaatca aacattgacc
     5941 acacggtaaa tagctgcgcg gttggcgaat acaaaagcga gttgagcggc gcggatatca
     6001 tcatcgcttc tacgcacatt gcgggcgaaa tcaccgtgac cggcaacaaa tacgtggttg
     6061 gcgtgcgcaa catgctctct cctgccgact ttggcccgaa actgctggaa gtgatcaaag
     6121 cgcatttccc gcaggatgtg aagtaaggac gcgccatgaa attacatgat tcgctggcgg
     6181 agaataaatc catccgcctg caggctgaag ctgagacatg gcaggacgca gtaaaaatcg
     6241 gcgttgacct gctggtggcg gcggatgttg tcgagccgcg ttactaccag gcgattctgg
     6301 atgcggttga acagcacggt ccgtactttg tgctcgcacc aggcctggca atgccgcacg
     6361 gtcgcccgga agagggcgtt aagaaaaccg gttttgcgct ggtgacgctg aaaaagccgc
     6421 tggagttcaa ccacgaagat aacgacccgg tggatatcct catcaccatg gcggcggtcg
     6481 atgccaacac gcaccaggaa gtgggcatca tgcagatcgt caacctgttt gaagatgaag
     6541 aaaatttcga ccgtttacgt gcctgccgta ccgagcagga agtactggat ctcattgacc
     6601 gcaccaacgc ggcagcttaa gaaggaattg aagatgtcat taccgatgtt gcaagtcgcg
     6661 ctggacaacc agactatgga tagcgcctac gaaaccactc gcctgattgc cgaagaagtc
     6721 gacattatcg aagtgggtac cattctgtgc gtgggcgaag gcgtgcgtgc ggttcgcgat
     6781 ctgaaagcgc tctacccgca caaaatcgtg ctggcagacg ccaaaattgc cgatgcgggc
     6841 aaaattcttt ctcgtatgtg cttcgaagcc aacgctgact gggtgaccgt gatttgttgt
     6901 gcggatatca acaccgccaa aggtgcgctg gacgtagcaa aagagtttaa cggcgacgtg
     6961 cagatcgaac tgaccggtta ctggacctgg gaacaggcgc aacagtggcg cgacgcaggt
     7021 attcagcagg tggtttatca ccgcagccgt gacgcgcagg ccgctggcgt ggcgtggggt
     7081 gaagccgata tcactgcgat caaacgtctt tccgatatgg gcttcaaagt caccgtcacc
     7141 ggtggcctgg cgctggaaga tctgccgctg ttcaaaggca ttccaattca tgtgtttatc
     7201 gccggtcgca gcatccgtga tgccgcttct ccggttgaag ccgcgcgtca gttcaaacgt
     7261 tccatcgccg aactgtgggg ctaaggagcg gatatgttgt ccaaacaaat cccgcttggc
     7321 atctatgaaa aagcgctccc cgccggggag tgctggctgg aacgcctgcg actggcaaaa
     7381 acgttgggct tcgattttgt cgagatgtcg gtagatgaaa ctaacgagcg cctgtcgcgc
     7441 ctcgactgga gccgcgaaca gcgtctggcg ctggtgaatg ctattgttga aaccggcgtg
     7501 cgtgtgccgt ccatgtgcct ttctgcccat cgtcgtttcc cgttgggcag cgaagatgac
     7561 gcggtgcggg cgcaggggct ggagattatg cgtaaagcca tccagttcgc ccaggacgtt
     7621 ggcattcgcg tgatccagct ggcgggctat gacgtttact atcaggaagc caataacgaa
     7681 acgcgtcgtc gtttccgtga cggcctgaaa gagagcgttg agatggcgag ccgcgcgcag
     7741 gtgacgctgg cgatggagat catggattat ccgttgatga actccatcag caaggcgctg
     7801 ggttacgcgc actatctcaa caacccgtgg ttccagctct acccggatat cggcaacctg
     7861 tcggcgtggg acaacgatgt gcagatggag ttgcaggccg gaatcgggca tatcgtcgcg
     7921 gtgcatgtga aagacaccaa accgggcgtc ttcaaaaacg tgccatttgg cgaaggtgtg
     7981 gtggatttcg aacgttgctt cgaaacgctc aaacagagtg gctattgcgg gccgtacctg
     8041 attgagatgt ggagcgaaac ggcggaagac ccggcggcag aagtggtgaa agcgtgtgat
     8101 tgggtgaaag cgcgcatggc caaagcaggc atggtggagg cggcataatg caaaagctaa
     8161 aacagcaggt gtttgaagcc aacatggatc tgccgcgcta tgggctggtg acctttactt
     8221 ggggcaacgt cagcgctatc gaccgcgaac gcgggctggt ggtgatcaag cccagcggcg
     8281 ttgcctacga aaccatgaaa gcagacgata tggtggtggt cgatatgagc ggcaaggtgg
     8341 tggaagggga gtatcgccca tcttccgaca ctgcgacgca tctcgaactc taccgtcgtt
     8401 acccgtcgct tggcggcatt gtccataccc actccactca tgccaccgca tgggcgcagg
     8461 cggggctggc gatcccggca ttaggcacca cgcacgctga ctacttcttt ggtgatatcc
     8521 cgtgtacgcg tggattaagc gaagaagagg tgcagggtga gtatgaactg aacactggca
     8581 aagtgattat cgaaacgctg ggcaacgccg agccgctgca tacgccggga attgtggtgt
     8641 atcagcacgg accgttcgcc tgggggaaag atgcccacga cgcggtgcat aacgcggtag
     8701 tgatggaaga agtggcgaaa atggcgtgga ttgcgcgcag tattaatcca caactcaatc
     8761 atatcgacag ctacctgatg aataaacact tcatgcgtaa gcacggtcct aacgcttatt
     8821 acgggcagaa gtaaaacacg cgctgcggaa atttccttcc tcgggagata actggtctaa
     8881 ttccgcagcc gtttttcaaa aaaagccccc tgcgaagggg gcaaagcaaa ctatggcaat
     8941 gtttcgttgg ttatacctgg tgctagcgat aaatatccgc gctggcgtgc atattgccgt
     9001 tactccccgg ttcccgcatc agaattacgt ggtagtacgt tgcgccttgc gcatctgttt
     9061 cttcatttaa tgcctgacgt gcttcgcttt cagtggcgaa attatgattg atataaatca
     9121 cgcctaagct ttgcacatcg tccatatttc tggcctggtg gttattaatt tcaatggctg
     9181 cccatgtatt tgcacttagc aaaagcacag ccagaagggc taaaactcga ctgaacatag
     9241 atacctcctc gacggctgac tttgtgtgct ctcctctgtg atgatcttct gatttaattt
     9301 taatcaatga taaagaagtt gatggtgacc atttctgatg cagttgttca aaaaaacact
     9361 gtgacggaat gtgatgaact tcaaatcagc ggtttagagg ttatttgcga aaggggagat
     9421 ttatttctgc tccgcccttg agtttagcgg ggtatgccag tactataacg cgtcattttt
     9481 cagccgacct ttaacacgtt ccttgcctcc ccgggattcg gctgacccag acaggaggct
     9541 gaataatccg taaggagcaa ttcgatgcgt cattacgaaa tcgtttttat ggtccatcct
     9601 gaccagagcg aacaggttcc gggcatgatc gagcgctaca ctgctgccat cactggtgca
     9661 gaaggcaaga tccaccgtct ggaagactgg ggccgccgtc agctggctta cccgatcaac
     9721 aaactgcaca aagcacacta cgttctgatg aacgttgaag ctccgcagga agtgatcgat
     9781 gagctggaaa ctaccttccg cttcaacgat gccgttatcc gcagcatggt tatgcgtacc
     9841 aagcacgctg ttaccgaagc atctccgatg gttaaagcga aagacgagcg ccgtgagcgt
     9901 cgcgatgatt tcgcaaacga aaccgctgat gatgctgaag ctggggattc tgaagagtaa
     9961 tttctgatga ccaaccgtct ggtgttgtcc ggcaccgtgt gcaggactcc ccttcgaaag
    10021 gtcagtccat caggaattcc tcactgccag ttcgtgcttg agcatcgttc tgtgcaggag
    10081 gaagccggct ttcaccggca ggcatggtgt caaatgcccg ttattgttag cggacacgaa
    10141 aaccaggcca ttactcacag tataacggtc ggcagtcgca taaccgttca ggggttcatt
    10201 tcatgccaca aggcaaagaa cggactgagc aaaatggttt tgcatgccga gcagattgaa
    10261 ttgatagatt ctggagacta gccatatggc acgttatttc cgtcgtcgca agttctgccg
    10321 tttcaccgcg gaaggcgttc aagagatcga ctataaagat atcgctacgc tgaaaaacta
    10381 catcaccgaa agcggtaaga ttgtcccaag ccgtatcacc ggtacccgtg caaaatacca
    10441 gcgtcagctg gctcgcgcta tcaaacgcgc tcgctacctg tccctgctgc cgtacactga
    10501 tcgccatcag taatcggtca cggtccatta atacgacttt gagaggataa ggtaatgcaa
    10561 gttattctgc ttgataaagt agcaaacctg ggtagcctgg gtgatcaggt aaacgttaaa
    10621 gcgggctatg ctcgtaactt cctggtaccg cagggtaaag ctgttccagc taccaagaaa
    10681 aacattgaat tcttcgaagc acgtcgcgct gaactggaag ctaaactggc tgaagttctg
    10741 gcagctgcta atgctcgcgc tgagaaaatc aatgcactgg aaactgttac catcgcgtct
    10801 aaagctggcg acgaaggtaa actgttcggt tccatcggta ctcgcgacat cgctgacgct
    10861 gtaactgcag ctggcgttga agtggctaag agcgaagttc gtctgccgaa cggcgttctg
    10921 cgtaccactg gcgaacacga agtgagcttc caggttcaca gcgaagtatt cgcgaaagtg
    10981 atcgtaaacg tagtagctga ataattcgtt attcaacgag acgtaaaaac gccgaccatt
    11041 ggtcggcgtt ttgctttcta tttttcgtca ggcattaatt tcgcaagtag atccattcac
    11101 cattctcttg tgtccataag tcaggatggt ttgctggcgt tagatgcata cgatcactcc
    11161 ttatgtctcg gtcctgcggc actcgctcga aacctaaacc cgagtagatt gcggctgaat
    11221 ttgataagga acgtagacca attttcaaat catggggtaa atcagaatac gcgtttagca
    11281 atgcagcaat gatgaatccg agggcattag aaacgcctgt aacagtgtga agttggtcga
    11341 tataaagatc gattgttggt agttcagatg agcagcaatt aggcatttgg gcaggaaggg
    11401 ttacaacgta tcctatcgtt tggccggtat agtctggagt actataacca ggtcccaaaa
    11461 caacctcgtg tattgtgtat ttactgttgg gttcgaagga ttcgacatct tgcaataaat
    11521 atttcagttc tgcggttaac ctcgcacccg cgtaagcttt attgtatttc tgggcgtgaa
    11581 tattatctaa tgtagcttca tggttccatc caaaagaatt atcaccattc attgttactg
    11641 aatcgccacg ggtttaacag acacctcaga gtcatttaag atgg
//
LOCUS       DAEBXN010000123        11250 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.123, whole genome shotgun sequence.
ACCESSION   DAEBXN010000123 DAEBXN010000000
VERSION     DAEBXN010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11250
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.123"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(1..386)
                     /locus_tag="KJL99_003503"
                     /pseudo
     CDS             complement(1..386)
                     /locus_tag="KJL99_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            910..2838
                     /gene="thrS"
                     /locus_tag="KJL99_003504"
     CDS             910..2838
                     /gene="thrS"
                     /locus_tag="KJL99_003504"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HBD4438097.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2842..3384
                     /gene="infC"
                     /locus_tag="KJL99_003505"
     CDS             2842..3384
                     /gene="infC"
                     /locus_tag="KJL99_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HBD4438098.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPST
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3481..3678
                     /gene="rpmI"
                     /locus_tag="KJL99_003506"
     CDS             3481..3678
                     /gene="rpmI"
                     /locus_tag="KJL99_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HBD4438099.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3731..4087
                     /gene="rplT"
                     /locus_tag="KJL99_003507"
     CDS             3731..4087
                     /gene="rplT"
                     /locus_tag="KJL99_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HBD4438100.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTTLVEKAKAALA"
     misc_feature    4204..4328
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            4210..4254
                     /gene="pheM"
                     /locus_tag="KJL99_003508"
     CDS             4210..4254
                     /gene="pheM"
                     /locus_tag="KJL99_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HBD4438101.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4537..5520
                     /gene="pheS"
                     /locus_tag="KJL99_003509"
     CDS             4537..5520
                     /gene="pheS"
                     /locus_tag="KJL99_003509"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HBD4438102.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVSIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5535..7922
                     /gene="pheT"
                     /locus_tag="KJL99_003510"
     CDS             5535..7922
                     /gene="pheT"
                     /locus_tag="KJL99_003510"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HBD4438103.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPVLQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLASPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7927..8226
                     /gene="ihfA"
                     /locus_tag="KJL99_003511"
     CDS             7927..8226
                     /gene="ihfA"
                     /locus_tag="KJL99_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HBD4438104.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8327..9307
                     /gene="btuC"
                     /locus_tag="KJL99_003512"
     CDS             8327..9307
                     /gene="btuC"
                     /locus_tag="KJL99_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HBD4438105.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPMWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9370..9921
                     /gene="btuE"
                     /locus_tag="KJL99_003513"
     CDS             9370..9921
                     /gene="btuE"
                     /locus_tag="KJL99_003513"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HBD4438106.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTTVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9921..10670
                     /gene="btuD"
                     /locus_tag="KJL99_003514"
     CDS             9921..10670
                     /gene="btuD"
                     /locus_tag="KJL99_003514"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HBD4438107.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10748..11212
                     /locus_tag="KJL99_003515"
     CDS             10748..11212
                     /locus_tag="KJL99_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBD4438108.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLHLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
BASE COUNT         2845 a         2617 c         3031 g         2757 t
ORIGIN      
        1 attaccctgt tcctgttcaa tataatccag tatgcttcgc aaatggttag ctttaatagg
       61 cgtggtttct agtaataagg aaatgatttc ttttttatag tgtaatgtaa tgagtatatt
      121 ttctgcatgg atgggtgaag tggcaaagtt aatggtaaaa agggcagtgg aataacaatt
      181 ttgtgatatt ttcgacagtt tgcggtaaat tcctgagtaa cacagtatga cagcatcgaa
      241 cttaagtcag aggtatcaag tagggtgatg agctggcgcg caaatttatt aataggctct
      301 gatgaaggtg ttggtatgtc gctatcgatg tgcaatctat gaggagactt ataatgagtt
      361 ctgataatga tatcgttttg actcataatc tattcgcctt atatatatct ggaggttttg
      421 aggatgatga ttaattatta tcgccagaac gaaatgtgca cgaaaaattg ttataagaat
      481 tgtgtcactt ttatcattgt gatatgacca cttcgtcatt aatctcatga gcgtttaaaa
      541 tactaccgcc aaatcacgca acgaactttg ctggattcag tttatgctgc tgtaaatccg
      601 ctcgcagtaa acctttcaga cgcaccgtga tgttatcagt ggttctttaa gcgttttgct
      661 ggtgtactca ctacaaacga attgcgaatc gaatcaatgt gaaacggaaa ggtacaatct
      721 cccttttttt agtgttaaca tcgttcaacc gggtggaagg tgagaggtta atgtctttca
      781 tttgtgtacc tgattgcgaa ccaatttagc atttgttggc tagatggttt cgcaatgaac
      841 tgttaataaa caaatttttc tttgtatgtg atctttcgtg tgggtcacca ctgcaaataa
      901 ggatataaaa tgcctgttat aactcttcct gatggcagcc aacgccatta cgaccacgct
      961 gtaagcccca tggatgttgc gctggacatt ggtccaggtc tggcgaaagc ctgtatcgca
     1021 gggcgcgtta atggcgaact ggttgatgct tgcgatctga ttgaaaacga cgcacaactg
     1081 tcgatcatta ccgccaaaga cgaagaaggt ctggagatca ttcgtcactc ctgtgcgcac
     1141 ctgttagggc atgcgattaa acaactttgg ccgcatacca aaatggcaat cggcccggtt
     1201 attgacaacg gtttttatta cgacgttgat cttgaccgca cgttaaccca ggaagatgtc
     1261 gaagcactcg agaagcggat gcatgagctt gctgagaaaa actacgacgt cattaagaag
     1321 aaagtcagct ggcacgaagc gcgtgaaact ttcgccaacc gtggggagag ctacaaagtt
     1381 tccattcttg acgaaaacat cgcccatgat gacaagccag gtctgtactt ccatgaagaa
     1441 tatgtcgata tgtgccgcgg tccgcacgta ccgaacatgc gtttctgcca tcatttcaaa
     1501 ctaatgaaaa cggcaggggc ttactggcgt ggcgacagca acaacaaaat gttgcaacgt
     1561 atttacggta cggcgtgggc agacaaaaaa gcacttaacg cttacctgca gcgcctggaa
     1621 gaagccgcga aacgcgacca ccgtaaaatc ggtaaacagc tcgacctgta ccatatgcag
     1681 gaagaagcgc cgggtatggt attctggcac aacgacggct ggaccatctt ccgtgaactg
     1741 gaagtgtttg ttcgttctaa actgaaagag taccagtatc aggaagttaa aggtccgttc
     1801 atgatggacc gtgtcctgtg ggaaaaaacc ggtcactggg acaactacaa agatgcaatg
     1861 ttcaccacat cttctgagaa ccgtgaatac tgcattaagc cgatgaactg cccgggtcac
     1921 gtacaaattt tcaaccaggg gctgaagtct tatcgcgatc tgccgctgcg tatggccgag
     1981 tttggtagct gccaccgtaa cgagccgtca ggttcgctgc atggcctgat gcgcgtgcgt
     2041 ggatttaccc aggatgacgc gcatatcttc tgtactgaag aacaaattcg cgatgaagtt
     2101 aacggatgta tccgtttagt ctatgatatg tacagcactt ttggcttcga gaagatcgtc
     2161 gtcaaactct ccactcgtcc tgaaaaacgt attggcagcg acgaaatgtg ggatcgtgct
     2221 gaggcggacc tggcggttgc gctggaagaa aacaacatcc cgtttgaata tcaactgggt
     2281 gaaggcgctt tctacggtcc gaaaattgaa tttaccctgt atgactgcct cgatcgtgca
     2341 tggcagtgcg gtacagtaca gctggacttc tccttgccgt ctcgtctgag cgcctcctat
     2401 gtgggcgaag acaacgagcg taaggtaccg gtaatgattc accgcgcaat tctggggtcg
     2461 atggagcgtt tcatcggtat cctgaccgaa gaattcgctg gtttcttccc gacctggctt
     2521 gcgccagttc aggttgttat catgaatatt accgattcac agtctgaata cgttaacgaa
     2581 ttgacgcaaa aactatcaaa tgcgggcatt cgtgttaaag cagacttgag aaatgagaag
     2641 attggcttta aaatccgcga gcacactttg cgtcgcgtcc catatatgct ggtctgtggt
     2701 gataaagagg tggaatcagg caaagttgcc gttcgcaccc gccgtggtaa agacctggga
     2761 agcatggacg taaatgaagt gatcgagaag ctgcaacaag agattcgcag ccgcagtctt
     2821 aaacaattgg aggaataagg tattaaaggc ggaaaacgag ttcaaacggc gcgccctaac
     2881 cgtatcaatg gcgaaattcg cgcccaggaa gttcgcttaa caggtctgga aggcgagcag
     2941 cttggtattg tgagtctgag agaagctctg gagaaagcag aagaagccgg agtagactta
     3001 gtcgagatca gccctaacgc cgagccgccg gtttgtcgta taatggatta cggcaaattc
     3061 ctctatgaaa agagcaagtc ttctaaggaa cagaagaaaa agcaaaaagt tatccaggtt
     3121 aaggaaatta aattccgtcc tagtacagat gaaggcgact atcaggtaaa actccgcagc
     3181 ctgattcgct ttctcgaaga gggtgataaa gccaaaatca cgctgcgttt ccgcggtcgt
     3241 gagatggcgc accagcaaat cggtatggaa gtgcttaatc gcgtgaaaga cgatttgcaa
     3301 gaactggcag tagtcgaatc cttcccaacg aagatcgaag gccgccagat gatcatggtg
     3361 ctcgctccta agaagaaaca gtaaggcctt caagtagcac tatctgtgga gcctttgggc
     3421 ttcacaggca ttgttcgcct ttgtttcgtt tattaacaat gcgaagtgga agttattaaa
     3481 atgccaaaaa ttaagaccgt acgcggtgct gctaagcgct tcaaaaaaac cggtaaaggt
     3541 ggttttaagc acaagcacgc taacctgcgt cacattctga ctaaaaaagc gaccaaacgt
     3601 aaacgtcacc tgcgtccgaa agccatggtt tccaaaggcg atctgggcct ggtaatcgcg
     3661 tgcctgccgt acgcataagc cgttaacgtt tttaactttt taattagaat atagatacag
     3721 gagagcacat atggctcgcg taaaacgtgg tgttattgca cgcgcacgtc acaagaaaat
     3781 tttgaaacaa gctaaaggct actacggtgc gcgttctcgc gtataccgcg ttgccttcca
     3841 ggctgttatc aaagctggtc agtatgctta ccgtgaccgt cgtcaacgta agcgtcagtt
     3901 ccgtcaactg tggattgcgc gtatcaacgc agcagcacgt cagaacggta tttcttacag
     3961 caaattcatc aatggcctga aaaaagcctc tgttgaaatc gaccgtaaga tcctggctga
     4021 tatcgcagta ttcgacaaag tagcgttcac cactctggtt gaaaaagcga aagcagctct
     4081 ggcataagcc agttgaaaga gggagctagt ctccctcttt tcgtttcaac gccatcaaaa
     4141 cattgacttt tatcgccgta gccttttcaa taaaggtctt ttgaagagta ccaaaaggta
     4201 acgcaagcaa tgaatgctgc tattttccgc ttcttttttt actttagcac ctgaatccag
     4261 gaggctagcg cgtgagaaga gaaacggaaa acagcgcctg aaagcctccc agtggaggct
     4321 ttttttgtat gcgcgttttg aaaaataaaa agccagtcta tcaggcgtaa ttgtcgcagc
     4381 cagtctggac acggccagtg cggagaaacc ggagcgtact taagtacgtg aggatttcga
     4441 gcactgcccg ggaccaaaat ggcaagtaaa atagcctgat gggataggct ctaagtccaa
     4501 cgaaccagtg tcaccactga cacaatgagg aaaaccatgt cacatctcgc agaactggtt
     4561 gccagtgcga aggcggccat tagccaggcg tcagatgttg ccgcgttaga taatgtgcgc
     4621 gtcgaatatt tgggtaaaaa agggcactta acccttcaga tgacgaccct gcgtgagctg
     4681 ccgccagaag agcgtccggc agctggtgcg gttatcaacg aagcgaaaga gcaggttcag
     4741 caggcgctga atgcgcgtaa agcggaactg gaaagcgctg cactgaatgc gcgtctggcg
     4801 gcggaaacga ttgatgtctc tctgccaggt cgtcgcattg aaaacggcgg tctgcatccg
     4861 gttacccgta ccatcgaccg tatcgaaagt ttcttcggtg agcttggctt taccgtggca
     4921 accgggccgg aaatcgaaga cgattatcat aacttcgatg ctctgaacat tcctggtcac
     4981 cacccggcgc gcgctgacca cgacactttc tggtttgacg ctacccgcct gctgcgtacc
     5041 cagacctctg gcgtacagat ccgcaccatg aaagcccagc agccaccgat tcgtatcatc
     5101 gcgcctggcc gtgtttatcg taacgactac gaccagactc acacgccgat gttccatcag
     5161 atggaaggtc tgattgttga taccaacatc agctttacca acctgaaagg cacgctgcac
     5221 gacttcctgc gtaacttctt tgaggaagat ttgcagattc gcttccgtcc ttcctacttc
     5281 ccgtttaccg agccttctgc agaagttgac gtcatgggta aaaacggtaa atggctggaa
     5341 gtgctgggct gcgggatggt gcatccgaac gtgctgcgta acgttagcat cgacccggaa
     5401 gtttactctg gtttcgcctt cgggatgggg atggagcgtc tgactatgtt gcgttacggc
     5461 gtcaccgacc tgcgttcatt cttcgaaaac gatctgcgtt tcctcaaaca gtttaaataa
     5521 ggcaggaata gattatgaaa ttcagtgaac tgtggttacg cgaatgggtg aacccggcga
     5581 tcgatagcga tacgctggcg aatcaaatca ctatggcggg cctggaagtt gacggtgtag
     5641 aaccggttgc cggtagcttc cacggcgtgg tcgttggtga agtggttgag tgtgcgcagc
     5701 atccgaacgc tgacaaactg cgtgtgacaa aagtgaatgt cggcggcgat cgcctgctgg
     5761 acatcgtctg cggtgcgcca aactgccgtc agggcctgcg tgtggcggta gctaccattg
     5821 gtgctgttct gccgggtgat ttcaaaatta aagcggcgaa actgcgtggc gaaccgtctg
     5881 aagggatgct gtgctccttt tctgagctgg gaatttctga cgatcacagt ggcattatcg
     5941 aactgcctgc ggatgcgccg attggcaccg acatccgcga atacctgaaa ctcgatgata
     6001 acaccatcga aatcagcgtg acaccaaacc gtgccgactg cttaggtatc attggtgttg
     6061 cgcgtgacgt tgccgtgctg aatcagctgc cgctggttga accggaaatc gttccggttg
     6121 gtgcgactat cgacgacacg ttgccgatta cagtcgaagc gccggaagcc tgcccgcgct
     6181 atcttggccg tgtggtaaaa ggcattaacg ttaaagcgcc aactccgctg tggatgaaag
     6241 aaaaactgcg tcgttgcggg atccgttcta tcgatgcagt tgttgacgtc accaactatg
     6301 tactgctcga actgggccag ccgatgcacg ctttcgataa agatcgcatt gaaggcggca
     6361 ttgtggtgcg gatggcaaaa gagggcgaaa cgctggtgct gcttgatggc actgaagcga
     6421 agctgaatgc tgacactctg gtcatcgccg accacaacaa ggcgctggcg atgggcggca
     6481 tctttggtgg cgaacactct ggcgtgaatg acgaaacgca aaacgtcttg ctggaatgcg
     6541 ctttcttcag cccgctgtct atcaccggtc gtgctcgtcg tcatggcctg catactgatg
     6601 cgtctcaccg ttatgagcgt ggcgttgatc cggtactgca gcacaaagcg atggaacgtg
     6661 cgacccgtct gctgatcgac atctgcggtg gtgaggctgg cccggtaatt gatatcacca
     6721 acgaagcaac gctgccgaag cgtgcaacca tcactctacg tcgtagcaaa ctggatcgcc
     6781 tgataggcca tcatattgct gatgagcagg taactgacat tctgcgtcgt ctcggctgcg
     6841 aagtgaccga aggcaaagac gaatggcagg cagttgcgcc gagctggcgt ttcgatatgg
     6901 agattgaaga agatctggtc gaagaagtcg cgcgtgttta cggctacaac aacatcccgg
     6961 atgagccggt acaggcaagc ctgattatgg gtactcaccg tgaagctgac ctgtcgctca
     7021 agcgcgtgaa aacgctgctc aacgacaaag gctatcagga agtgatcacc tacagcttcg
     7081 ttgatccgaa agtgcagcag atgatccatc caggcgttga agccttactg ctggcaagcc
     7141 cgatctctgt tgaaatgtca gcaatgcgtc tttctctgtg gaccggcctg ctggcaaccg
     7201 tggtgtacaa ccagaaccgt cagcagaacc gtgtgcgcat tttcgaaagc ggtctgcgtt
     7261 tcgtaccaga tactcaggca ccgttgggca ttcgtcagga tctgatgtta gccggtgtga
     7321 tttgcggtaa ccgttacgaa gagcactgga acctggcaaa agagaccgtt gatttctatg
     7381 atttgaaagg cgaccttgaa tccgttctcg acctgaccgg taaactgaat gaggttgagt
     7441 tccgtgcaga agcgaatccg gcactgcatc cggggcaatc cgcagcgatt tatctgaaag
     7501 gtgaacgtat tggttttgtt ggagttgttc atcctgaact ggaacgtaaa ctggatctta
     7561 acggtcgcac tctggtgttc gaactggagt ggaacaagct cgcagaccgc gtggtgcctc
     7621 aggcgcgcga gatttctcgc ttcccggcga accgtcgtga catcgcggtg gtggtcgcag
     7681 aaaacgttcc cgcagcggat attttatccg aatgtaagaa agttggcgta aatcaggtag
     7741 ttggcgtaaa cttatttgac gtgtaccgcg gtaagggtgt tgcggagggg tataagagcc
     7801 tcgccataag cctgatcctg caagatacca gccgtacact cgaagaagag gagattgccg
     7861 ctaccgtcgc caaatgtgta gaggcattaa aagagcgatt ccaggcatca ttgagggatt
     7921 gaacctatgg cgcttacaaa agctgaaatg tcagaatatc tgtttgataa gcttgggctt
     7981 agcaagcggg atgccaaaga actggttgaa ctgtttttcg aagagatccg tcgcgctctg
     8041 gaaaacggcg aacaggtgaa actctctggt tttggtaact tcgatctgcg tgataagaat
     8101 caacgcccgg gacgtaaccc gaaaacgggc gaggatattc ccattacagc acggcgcgtg
     8161 gtgaccttca gacccgggca gaagttaaaa agccgggtcg aaaacgcttc gcccaaagac
     8221 gagtaatctg atctaactaa aaaggccgct atgcggcctt ttttcttttc actgtcgaag
     8281 agtcaccgta aaatcaacgc catgacactt cagcagaacg gataccatgc tgacacttgc
     8341 ccgccaacaa cagcgacaaa atattcgctg gttattatgc ctgtcagttt tgatgctgct
     8401 ggcgcttctc ttaagccttt gcgccggtga acaatggatt tcgccaggtg actggtttac
     8461 tcctcgtggc gaactgttcg tctggcaaat tcgcctgcca cgtacgctgg ctgtattgct
     8521 ggttggtgcg gcgctggcta tatccggcgc tgtaatgcag gcgttgtttg aaaatcctct
     8581 ggcagaacct ggactacttg gcgtctctaa cggcgcaggc gtggggctta tcgccgcggt
     8641 attgcttggg caagggcaac tccccaactg ggcgctaggg ctgtgtgcga ttgctggcgc
     8701 gcttatcatc actttaatac tcttacgttt cgcccgtcgt catctttcga ccagtcggtt
     8761 attgctggct ggcgttgcat tagggattat ctgcagcgca ctaatgacgt gggctatcta
     8821 cttttccacc tctgttgatt tacgtcagct gatgtactgg atgatgggcg gttttggcgg
     8881 cgtagactgg cggcaaagct ggctgatgct ggcattgatc cccgtgttgt tgtggatctg
     8941 ttgtcagtcc aggccgatga atatgttagc gcttggcgag atctcggcgc ggcaactggg
     9001 tttacccatg tggttctggc gcaatgtgct ggtggcagcg accggctgga tggttggcgt
     9061 cagtgtggcg ctggcgggtg ctatcggctt tattggtctg gtgatcccac atattcttcg
     9121 gttgtgtggt ttaaccgatc atcgcgtatt acttcccggc tgcgcgctgg caggggcgag
     9181 cgcattgctg ctggccgata ttgtagcgcg cctggcatta gctgccgcag agctgcctat
     9241 tggcgtggtc accgcaacgt tgggtgcgcc ggtgtttatc tggttattgt taaaagcagg
     9301 acgttagccg caaaaagacg gtctatgatt aaaagctaag atttttactg accacaccta
     9361 ggagaaacga tgcaagattc cattctgacg accgtagtga aagatatcga cggtgaagtg
     9421 accacgctgg agaagtacgc cggtaatgtg ctgttaattg tcaatgtcgc ctcaaagtgt
     9481 ggcttaacgc cgcaatatga gcagttggag aatattcaaa aagcctgggc cgatcgaggt
     9541 tttgtggtgc tgggattccc gtgcaaccag tttctggaac aagaaccggg cagcgatgaa
     9601 gagattaaaa cttactgtac caccacatgg ggggtgacgt tcccgatgtt cagtaagatt
     9661 gaagttaatg gcgaaggacg ccatccgctg tatcaaaaat tgattgccgc agcgccgacc
     9721 acagtcgcgc cggaagagag cggattctat gcccgtatgg tcagcaaagg ccgtgcaccg
     9781 ctgtacccgg atgatatttt atggaatttt gaaaaattcc tggttggcag ggacggaaaa
     9841 gtcatccagc gtttttcccc ggatatgacg ccggaagatc ccattgtgat ggaaagcatt
     9901 aaactggcgt tggcaaaata atgtctattg tgatgcagtt acaggatgtt gcggaatcta
     9961 cccgcctggg gccgctttca ggcgaggttc gggctgggga aatcctgcac ctggtggggc
    10021 cgaatggcgc gggtaagagt accttactgg cgcgaatggc cggaatgacc agcggtaagg
    10081 gaagcattca gttcgcgggg caaccactgg aagcatggtc cgcaacaaaa ctcgcgctgc
    10141 atcgcgccta tctttcacaa cagcagacgc cgccgtttgc aatgccggtc tggcactacc
    10201 tgacactgca tcagcacgat aaaacgcgta ccgaactact gaatgatgtc gcaggggcgc
    10261 tggctcttga tgacaaactc ggtcgtagca ccaatcaact ttccggcggt gaatggcaac
    10321 gcgtacgtct tgctgcggtg gtgttgcaaa tcacaccaca agccaatccc gcaggccaat
    10381 tgctgcttct tgatgagccg atgaacagtc ttgatgttgc gcaacaaagt gcgttagaca
    10441 aaattctgag cgcgctgtgt cagcaaggac tggcgattgt gatgagcagt cacgatctca
    10501 accacacgtt gcgtcatgcg catcgggcgt ggttgctaaa aggtggaaaa atgctggcca
    10561 gtggacgcag agaagaggtg ctcacgccgc caaatctggc gcaggcctat gggatgaatt
    10621 ttcgccgtct ggatatcgaa ggtcacagaa tgctgatttc gaccatctga tttcattagt
    10681 atcccgaaaa tcgggtaatt acgacgctaa attaatgcaa gaataaaaaa cagaggaacc
    10741 gtacggaatg cgtttctgcc ttattttgat cacagcactg tttctggccg ggtgtagcca
    10801 ccataaagca ccgccgccaa atgccagact ttctgattcg attaccgtta ttgccggttt
    10861 gaacgaccag ctacaaagct ggcatggcac gccgtatcgt tatggtggca tgacgcggcg
    10921 cggtgtggac tgttcgggat ttgtggttgt gacgatgcgc gatcgtttcg atttgcatct
    10981 gccccgtgaa accaaacaac aagcctctat tggcacgcaa attgataaag acgagttgct
    11041 gcctggtgac ctggtctttt tcaaaacggg ttccggacaa aatggtttgc atgtaggtat
    11101 ttacgatacc aacaaccaat ttatccacgc ctctaccagc aagggagtga tgcgttcctc
    11161 acttgataat gtctattggc agaaaaattt ctggcaggcg agacgaatct agtatgtcga
    11221 aaaattgacg gcgatgatac atcgccgtca
//
LOCUS       DAEBXN010000124        11048 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.124, whole genome shotgun sequence.
ACCESSION   DAEBXN010000124 DAEBXN010000000
VERSION     DAEBXN010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11048
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.124"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            409..1239
                     /locus_tag="KJL99_003516"
     CDS             409..1239
                     /locus_tag="KJL99_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HBD4438109.1"
                     /translation="MSMPLSNAANNQFNTNNHFLHHPKVDSELTRRYEYARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLNIGKQSWPFNYVTDTCREFNGITTTGRMPYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIGIEAYPGTAEIHSKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1380..2153)
                     /gene="uxuR"
                     /locus_tag="KJL99_003517"
     CDS             complement(1380..2153)
                     /gene="uxuR"
                     /locus_tag="KJL99_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HBD4438110.1"
                     /translation="MKSVTSAQRPYQEVGAMIHDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2368..3828)
                     /locus_tag="KJL99_003518"
     CDS             complement(2368..3828)
                     /locus_tag="KJL99_003518"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HBD4438111.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRMLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GTQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMGYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTSAYQQLCERGARECVAAL"
     gene            complement(3909..5093)
                     /gene="uxuA"
                     /locus_tag="KJL99_003519"
     CDS             complement(3909..5093)
                     /gene="uxuA"
                     /locus_tag="KJL99_003519"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBD4438112.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYKVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5433..6776
                     /gene="gntP"
                     /locus_tag="KJL99_003520"
     CDS             5433..6776
                     /gene="gntP"
                     /locus_tag="KJL99_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HBD4438113.1"
                     /translation="MHVLNILWVVFGIGLMLILNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(7027..7929)
                     /gene="fimH"
                     /locus_tag="KJL99_003521"
     CDS             complement(7027..7929)
                     /gene="fimH"
                     /locus_tag="KJL99_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HBD4438114.1"
                     /translation="MKRAITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7949..8452)
                     /gene="fimG"
                     /locus_tag="KJL99_003522"
     CDS             complement(7949..8452)
                     /gene="fimG"
                     /locus_tag="KJL99_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HBD4438115.1"
                     /translation="MKWCKRGYVLAAMLALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(8465..8995)
                     /gene="fimF"
                     /locus_tag="KJL99_003523"
     CDS             complement(8465..8995)
                     /gene="fimF"
                     /locus_tag="KJL99_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HBD4438116.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(9005..>11048)
                     /gene="fimD"
                     /locus_tag="KJL99_003524"
     CDS             complement(9005..>11048)
                     /gene="fimD"
                     /locus_tag="KJL99_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HBD4438117.1"
                     /translation="RIGGNSHYAYLNLQSGLNIGAWRLRDNTTWSYNSSDSSSGSKNK
                     WQHIITWIERDIIPLRSRLTLGDGYTQGDIFDGINFRGAQLASDDNMLPDSQRGFAPV
                     IHGIARGTAQVTIKQNGYGIYNSTVPPGPFTINDIYAAGNSGDLQVTIKEADGSTQIF
                     TVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEKPRFFQSTLLHGLPAGWTIYGGTQL
                     ADRYRAFNFGIGKNMEALGALSVDMTQANSTLPDDSQHDGQSVRFLYNKSLNESGTNI
                     QLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGVIQVKPKFTDYYNLAYNKRGKLQLT
                     VTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGLNTAFEDINWTLSYSLTKNAWQKGR
                     DQMLALNVNIPFSHWLRSDSKSQWRHASASYSMSHDLNGRMTNLAGVYGTLLEDNNLS
                     YSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANIGYSHSDDIKQLYYGVSGGVLAHAN
                     GVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTDWRGYAVLPYATEYRENRVALDTNT
                     LADNVDLDNAVANVVPTRGAIVRAEFKARVGIKLLMTLTHNNKPLPFGAMVTSESSQS
                     SGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCVANYQLPPESQQQLLTQLSAECR"
BASE COUNT         2685 a         2829 c         2683 g         2851 t
ORIGIN      
        1 acgtagatga aaatacttgt caccgctgct acttaattcc ggtgggaaat tatttatttc
       61 cgcataatca gctatgtatt atttgaaaca attagtttgt aaataatata aattgataac
      121 atatcaaata catcaaaaat accattcaca ggcattatta atatagacct caatatagca
      181 atatcaaatt ccaattagta ataagatatg cttttaaatt gcatgcaaat aattttaaat
      241 gagcgttttt tgatacttta gagtgtttcc taccctttaa tatcactgtg tagagttgat
      301 atcactacaa cagaaacatg catgtcattt gtttacttct ctttttacta cagaaaaatt
      361 tatctatttt tctaaacatc agttaaatac tcataaaagg taactattat gtctatgcca
      421 ttaagcaatg ccgctaataa ccaattcaat acaaataatc acttccttca tcatccaaaa
      481 gtcgacagtg agttaacacg tcgatatgaa tatgcacgcc tggatacgga aaatatctat
      541 cttctgcctc ttgctcgtgg caataaccat aattacgatg gaaaaagcgt tgtcgaaatt
      601 agaaaactta atattggcaa acagtcctgg ccatttaatt atgtgacaga tacatgcaga
      661 gaattcaatg gtattacaac aacaggacgt atgccctatc gaaacttgaa aatcacctca
      721 gctttagacg aaatatatgg tggtatatgt aaaaaggcgc atgctgctac agagttagca
      781 gaaggtttac ggctaaattt atttatgaag agccctttcg atcctgttga ggactataca
      841 gttcatgaaa taacattagg ccctggttgc aatgttcctg gttatgccgg aaccacgata
      901 ggatatattt caacccttcc agcttctcag gcaaaaagat ggaccaatga gcagccaaga
      961 attgatatct atattgatca gataataact gtctcagggg tagcaaattc ttccggattc
     1021 gcccttgccg cattacttaa tgctaatatt gagatgggaa atgacccaat cattggtata
     1081 gaagcctatc ctggtaccgc ggagatccat tcaaaaatgg gatataacgt tatccccggt
     1141 gatgaagatg cgccactcaa acgcatgact ctgcaaccgt catctcttcc agaactgttt
     1201 gaattaaaaa atggtgaatg gaattatatt ggtaaatagc cactgagaag aatgagtact
     1261 aatattcagg ttatacagat atttaaacaa aaccctgcaa tttacaaagt aatactcatt
     1321 gcgacttctt aatttaagaa agcgcaagga acgtttaccc ttgcgcttat tataataagt
     1381 caggcgtcga ctttatccag cggccatgaa tcaaagagat agccgtcaaa ataaatatcg
     1441 tcaacgttcg agaattccag cagacgttgc ttaacgtttt ccagatgctg ccacattgcc
     1501 agcttcgccg ctcgggcatc ttttttgatt aacgcggcga ggatctgttt gtgatcgccc
     1561 aaccactctt tgcgatacag gctgtcatcc agatggctgt gcaactggat ccacattgga
     1621 ttgttttccc gccactgcca ggactgacgg aataactcca ccagcatgct gttatgcgtt
     1681 gcttcagcaa tggcgagatg gaactgcatg tcaccgcttt cgctgctacc cggcgcgctg
     1741 gaagccagtt cacgctcttc cagttgcaat gcctgacgca ttttgacgat atcttcgcgg
     1801 gtagcctgca gtgcggcaaa ctcggcgatg ttactttcca gtaactgacg cgcctgtaac
     1861 agctcaaaag gacccgcatc attgcagacg ttggcatccg gactgtctgt gttgtggctg
     1921 cctgagctat caagcacata gataccggca ccccggcgca cttccaccag ccctttgatc
     1981 tccagcatga tcagcgcttc acgtaccacc gtccgcgtga catcaagcat ttctgcaatt
     2041 tcacgctccg gcggcagccg ttcgccagga ttgtacggcg tctttatgat cagatcgtgg
     2101 atcatcgccc cgacttcctg gtaaggtctt tgcgcagagg tgacagattt catggtgttg
     2161 tgtccgttag gtgcggttaa tcaaattttt gccggatgcg gcgtgaacgc cttatccggc
     2221 ctacaacggg caattttacg gcccccgtta atctcaaact ataccattag cactggaagt
     2281 tggcacgtac aggtgggcaa aaaaacgaaa actggtcaac caaatatcag tttgattgac
     2341 cagtaagtct gtagggtaat cagttagtta cagcgcagcc acacactcgc gcgcaccgcg
     2401 ttcgcacagc tgctgatatg ccgacgtcac cgcgccaaca aagtcggcat tctgcggcag
     2461 atcatcggca aaaataccgc tcaggcccag cagcgctttc acgcggtctg cgccctgata
     2521 ctgcgcgtta atcttctgga actcggccag catcgggtcg accacgtcaa tggcattacc
     2581 ctgctcatcc acgccctggg tgtaacccat ccagccagcc acgcccagcg ccaggtgacg
     2641 ccagctgccg ccgttttgca ggtgcagacg caccgggtcc agtagacgct gcggtaactt
     2701 ctggctgccg tccatcgcaa tctgccaggt acggtgacgc agagacgggt tgctgaaacg
     2761 ctcgatcagc agcgtcgcat aggcgttcag gtccgtacct tccggcatcg acagcgttgg
     2821 cgcttgttcc tgcatcatca gggcaaaggc cgctttgcga taatccgggt tagtcatggt
     2881 gtcggcaatg gtttcatagc cgccgaggta accgaggtac gccaggaaag agtggctgcc
     2941 gttcagcata cgcagcttca tcatttcgaa cggcacaacg tctgcaacga actgtgtgcc
     3001 cactttatcc cagtccgggc gaccattaac gaagttatct tcaatcaccc actgacggaa
     3061 cggttcgcag gcaatggcgc acgggtcgta aacacccagc tggtcagcaa tttcctgtaa
     3121 ggtttctggc gtcgccgccg gaacgatgcg gtcaaccatg gtgcacggga aggtgacgtt
     3181 ctcttcaatc catgccgcta gctgcgggtc acgcgcctga gccagcccca gtaccgcgac
     3241 cttcgccaca tggccgtttt cacgcacgtt atcgcaggac atcaccgtaa atgctttcag
     3301 ccctttttca cgacgcagac gcaaggcttc gacgatgtaa ccaatcgcgg acttcggcgc
     3361 agtcgggttt tccagatcgt gcttgatcag cgggttattg agatccagtt gaccgcttgc
     3421 cgcatcagcg cagtagcctt tttccgtgac cgttagagag acaatcgccg tttgcggacg
     3481 cgccatcgcg ttgagaatac cttcgcagcc gtcgatttcc ggatgcagcg cttctttcat
     3541 cgaaccgata attttcagct cggtgctttc tgcgcctttt tccgctacgg tgtacagcag
     3601 ttgctgtttt ttcaggtttt cgatcagcat gcggtcgttg cctggcatca ggttaacttc
     3661 gcagatgccc cagtcgctgt cggtgctttc cagcagatga tgggtataca gcgcctggtg
     3721 cgcgcggtga aacgccccgc aaccgagatg cacaatgcgt gattccagac gagaatgatc
     3781 ccatgacggg cgggcaaccg gcagattgct gtcaacaata gtagtcatcg taaactccag
     3841 aatccgtctt cagagagtga cggcaaggaa gaaggaaccg gcacggcgcg cagccatgcc
     3901 ggtgggtatt aacggctaaa gaaagcgcgc tggatcgcca gttcgacacc gcgaacttcg
     3961 gccaggcctt tcagacgacc aattgcggag taaccagggt tggttttctt cttcaggtcg
     4021 tccagcatct gatgaccgtg gtccggacgc atcgggatca ggtcttcttt gccttccgct
     4081 ttacgacggt gttcttcttc aacaatcgct ttcaccactt tgtacatatc aacgtcaccg
     4141 ttcaggtgcg ccgcttcgtg gaaggttttc gggttatctt cacgcatggt ggagcgcaga
     4201 tgggtgaagt aaatacgcgg accgaactgc ttgatcatat caaccagatc gttgtcagca
     4261 cgcacgccgt aggaaccggt gcacatggtg aaaccgttcg ccatgctgtt tacggtatca
     4321 accatccact gcatatcttc aatggtggaa acaatgcgcg gcaagccgag gatcgggcgc
     4381 ggcggatcgt ccgggtgaac cgccatacgc acgccaactt cttcagcaac tggaataatc
     4441 gctttcagga agacggcaaa gttttcgcgc agtttggctt tgtcgatatc tttgtacagc
     4501 tccaggtgct tacggaactg gtcgagggta tacccttctt ccgcgcccgg cagaccggca
     4561 atgatgttac gcgtcagacg cgctttgtct tcgtcgctca tagtggcgaa gcgttcagct
     4621 gcctgagcaa tttcttcttc ggtgtaatcc gcttccgcgc ctggacgttt caggatatgc
     4681 atttcgaatg cagcgaattc gatctggtcg aagcgcagag ctttggagcc gtctggcagc
     4741 acgtattcga ggtcagtacg ggtccagtcg agcaccggca tgaagttgta gcacacggtg
     4801 cgaatgccac actgcgccag gttgcgcaga gtctgctgat agttagcgat ccactgctca
     4861 tagttgccag tgtgggtttt gatatcttcg tgaattggta cgctttctac gacagaccac
     4921 accaggcctg cgtcttcaac gatcgccttg cgtttgagga tctcttctac ggaccatact
     4981 tcgccgttcg ggatatggtg cagcgcggta accacgccag ttgcgcccgc ctgacggaca
     5041 tcagctaaag aaaccggatc gtttgggccg taccagcgcc aggtctgttc catgattttt
     5101 cctctttcac atagtgggtt ccacagcaaa gagacgcgga accttggagt tgtttaggta
     5161 tccggatgaa caaccagacc gcagccgtgg cgttgcatcg gatgagatgg cgtataagtt
     5221 ctaccttttg gtaagacatg tcaaccagaa ttctgaaatt ggttaaccgc gtcgcaagaa
     5281 tttcactctc atggttgttg ctgcaaattg cagcaaaggg taatcgatgg ttattgatca
     5341 gaggatgtga cattcatcgc aacaatggtt gaccaattta cataacatgt cgcccaaaat
     5401 aacaacggtt caaccaagta aggtaaaacg ttatgcatgt gcttaacatt ctctgggtgg
     5461 tattcggcat tggcctgatg ctgatactga atttgaagtt caaaatcaat tcaatggtgg
     5521 ctttgttggt ggcggcgctg tccgtcggga tgctggcagg catggatttg atgtcgctgc
     5581 tgcacaccat gaaagcgggc ttcggcaaca cgctgggaga actggctatc atcgtggtgt
     5641 tcggtgcggt catcggtaaa ttgatggtcg actccggcgc ggctcaccag atagcacata
     5701 cgctgctggc gcgtctcggt ctgcgctatg tacagctgtc ggtgattatc atcggcctta
     5761 ttttcggtct ggcgatgttc tatgaagtgg cctttatcat gttagcgccg ctggttattg
     5821 ttattgccgc cgaagctaaa attccgttcc tgaaactggc gatcccggca gtagcagctg
     5881 ccactaccgc acattcactg ttcccaccgc agccgggtcc ggtggcgctg gtgaatgctt
     5941 atggcgcgga tatggggatg gtttatatct atggcgtact ggtgacgatc ccaagtgtaa
     6001 tctgcgcagg tctgatcctg ccgaagttcc tcggcaatct tgagcgccca acgccatcat
     6061 tcctgaaagc agatcaaccg gtagatatga ataatctgcc ctctttcggc gtttcgattc
     6121 tggtgccgct gatcccggcg atcattatga tctctaccac catcgccaat atctggctgg
     6181 taaaagatac ccctgcctgg gaagtggtta actttattgg ttcctcgccg attgcgatgt
     6241 ttattgcgat ggtggttgca ttcgtactct ttggcaccgc gcgtggtcat gacatgcagt
     6301 gggtgatgaa cgcttttgaa agcgcggtga agagtattgc aatggtgatt ctgatcatcg
     6361 gtgcgggtgg cgtgctgaag cagaccatca tcgacaccgg cattggcgac accatcggca
     6421 tgttgatgtc ccacggcaat atctcgccct acattatggc atggctgatc actgtgctaa
     6481 ttcgtctggc gacgggtcag ggtgtcgttt cggcgatgac cgccgccggg attatcagtg
     6541 ctgcaatcct tgatccagca accggtcagc tggttggcgt gaatccggcg ctgctggtac
     6601 tggcgacggc tgcgggttcc aacaccctca cccacattaa tgatgcatct ttctggttgt
     6661 tcaaaggtta ctttgacctg tcagtaaaag acacgttgaa aacctggggt ctgctggagc
     6721 tggtcaactc cgtggttggg ctgattattg tgctgattat tagcatggta gcgtaaaaga
     6781 acaccagagc ctgccaatgg caggctcaga ctgatgacta aagtctaaaa atgtgcccgg
     6841 acagtcccct cgcccctccg gggagagggt tagggtgagg ggaacagacc agtaaagtgc
     6901 gctttgttta tgccagatgc gacgctgacg cgtcttatct ggcctacaaa gggctaacgt
     6961 gcaggttttg taggtctgat agcgtagcgc ctcaggtacc agcattagca atgcgctgta
     7021 atttttttat tgataaacaa aagtcacgcc aataatcgat tgcacattcc ctgcagtcac
     7081 ctgccctccg gtacgtgcgt aatttgccgt taatcccaga cttaccgccg aagttcctac
     7141 tgctcctaac gataccgtgt tattcgctgg aataatcgta ccgttgcgcg tcaactgtac
     7201 gccgacgccc tgcgctggtg aaaacgacgc ggtattggtg aaaatcgagt tgcccgcatc
     7261 tgcggttgtg ccggagaggt aataccccag gttttggctt ttcgcacaat aaacggtaag
     7321 aggaattggc actgaaccag ggtagtccgg cagagtaacg gtgacatcac gagcagaaac
     7381 atcacaaccg ccagtgggca ccaccacatc attattggcg taaatattcc acacaaactg
     7441 aaaatcatcg ctgttatagt tgttggtctg tcgcaaaata agcacggcaa ttaatgaacc
     7501 agctttaata gccaccccgc ccgcactgct cacaggcgtc aaataaagcg ccaccggcca
     7561 cggcttatcc gttctcgaat tataaacaac ccgcggcgtt tcgctggtgg tcggaaatgg
     7621 atagctactg ccactatatt ttacggtccc ggaaaaatta gataacacgc cgccgtaagc
     7681 cgagcctcgt tgcagtgtga catagtctgt aatggtttcc ggataatcgt tatggcaaaa
     7741 gatttgcgtc gaaagatcta cgaccaggtt ttgccccaca ttcacgacag gcgcaaggtt
     7801 tacataaaca ttagcgctgc caccgccaat agggatagcg gtaccattgg cggttttaca
     7861 ggcgaatgac caggcattta ccgaccagcc catcagcagt acagcaaaca gggtaatagc
     7921 tcgtttcatt acaattatct cttcgggttc agctgtaggt ataggtgatg ctaatcactg
     7981 cctgaatggt tccctgagtg gctccgccat ttaccgtcaa tgctctgacc tgtaacggga
     8041 agtgcgctga ttgtgaggaa tcatccacct gaactgtttt ggttgcgcca gtattcaatg
     8101 tgttgccact gtcatcctgt agctctaact ggatgttttg cgcggtcccc tggtttttat
     8161 aatatccggt gctgtcggct gccccgctga agctggcagt gaccctcgac gttcccaccg
     8221 gacaattagt caactcaagc gcaacatcat gccaggccga tgccgccccg gcagacataa
     8281 gactgaaaga ataaagatcg ccgagatcta ccgtggcatt ggtggtggaa accgtacacg
     8341 gtttggcgac gaccttaccg ttcaccgtga tggtgacatc ggctgcctgt atcgtcgcac
     8401 ttgcgagcgc caacattgcc gccaatacat acccacgttt gcaccatttc atgagcatct
     8461 ccagttactg atattcaaga gtgaaggtag ccgtagcatt gatatgcccc gcagtgacag
     8521 gcacctgtgt cgccattagc cgggcgtaaa aattcagcgt atttggttta cccggcgtca
     8581 gggtcgtcca cgaaatcgca gacgatggag cattaagggg tatttgattt tgctgctcat
     8641 tcagaagctg tattcccagt cccgcagccg ctgacaccgt attttcaagt gcaagcaggt
     8701 tggcattgtg gctatctgca acgccggtaa acccaacttt tacggcagaa acggcattac
     8761 cacagggtga cagcaaaata cgaaatggaa cgacaggagt cgtcgcgcca atgttgttaa
     8821 attgcttcgc cgcgttttcc atcagatcaa cagtaaaatt ggttgattca gcggccacac
     8881 tacagccgtt atctctgaca tagccgcgga tagtaatcgt gctatccgca gccaaaacgc
     8941 gacttaccgc cagccacaaa aaagcgcaca gaagataaaa aggtttgttt ctcatcacgc
     9001 ccccttaacg acattcagct gatagctggg ttaataactg ctgctgactc tctggtggca
     9061 gttgataatt ggcgacacag tgagcatttt cctcttctcc ccatttcacc tgaacttttc
     9121 ccgctaaagg cattccgctg aggtaaacct gaccattatc cgcaacaatg ccgctactct
     9181 ggctactctc tgatgtcacc atcgccccaa acggcagcgg cttattattg tgagttagcg
     9241 tcatgagcag ttttatccca acgcgcgctt taaactctgc tcgcacgatc gccccacgag
     9301 tgggaacaac gttagcgacc gcgttatcta aatcgacgtt atcagccaag gtattggtat
     9361 ccagcgccac tctattttcc cgatattcag tggcataagg cagcacggca taaccacgcc
     9421 agtcggtacg cacccccgtc tggttttcga cttttgcatc ttttgcgcca ggcgctttaa
     9481 caagcaccac cgtatcgttt aacggctgcc ccagcgttac gccattggca tgagccaata
     9541 ccccaccgct gactccgtaa tagagctgct taatatcatc gctatggctg taaccgatat
     9601 tggcattgcc gtaaccaccg cgataattca gcgtggcgta gcctgtactt ccgctattac
     9661 catcgcctcc cccggcatag ccggtttgca cgctataact gaggttgttg tcttccagca
     9721 acgtaccgta tacaccagcc agattggtca tccgaccgtt gagatcgtgt gacatgctgt
     9781 agctggcact ggcatgtcgc cactgagatt tactgtcaga acgcagccag tggctgaaag
     9841 gaatattgac gttaagcgct aacatctgat cccgtccttt ttgccaggcg tttttcgtca
     9901 ggctatagct gagcgtccag ttgatatctt cgaacgcagt atttaatcca gcctggaatt
     9961 gctcatcgac attactcgtt ccccaataag tttgatggct accactcaaa tacagtgttg
    10021 atgtgcgccc gagttgctga gtaacggtga gctgtaattt cccgcgtttg ttataagcga
    10081 ggttgtaata gtcggtgaat ttcggcttaa cctgaataac tccatcctgt gtttcgatgt
    10141 tgtagccatt cattcgactg tatgttgtat cagcgaaatt aaaatatccg ctggtcgaat
    10201 aacggtaacc cactaactga atattcgtgc ctgattcatt gagcgatttg ttatagagaa
    10261 aacgcaccga ttgtccgtca tgctgactgt catcgggaag tgtggaatta gcctgcgtca
    10321 tatccacaga cagagcgccc agtgcctcca tgtttttccc gataccgaaa ttaaaagcac
    10381 gataacgatc cgccagttgc gttccaccat atattgtcca gccagccgga aggccgtgga
    10441 gtaatgtact ctggaaaaag cggggttttt cctgctgcgc atttccacta cggtattctc
    10501 ctgccgtaat ggaataacga gtatgccctt cacgttgcaa aagcgggact gacgaatagg
    10561 gtacggtaaa aatctgcgtg ctgccgtcag cctctttgat cgttacctgc aagtcaccac
    10621 tattacctgc ggcatagata tcgttgatgg taaaaggccc cggtggcacc gtactattat
    10681 aaatgccata tccattttgt ttaatagtga cctgtgcagt accacgagca ataccgtgga
    10741 tcaccggggc aaatcctctt tggctatcgg gtaacatatt gtcatctgag gccaattgtg
    10801 cgccgcgaaa gttaatacca tcgaaaatat cgccctgggt ataaccatca cccaacgtca
    10861 gccgggaacg taaaggaatt atgtctcgct caatccaggt aatgatatgc tgccatttat
    10921 ttttgctacc tgatgaactg tcgctactgt tataactcca ggtggtattg tcgcgtaaac
    10981 gccacgcacc aatatttaac ccactctgta ggtttaaata tgcataatgg ctgttacccc
    11041 caatccga
//
LOCUS       DAEBXN010000125        10970 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.125, whole genome shotgun sequence.
ACCESSION   DAEBXN010000125 DAEBXN010000000
VERSION     DAEBXN010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10970
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.125"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            342..722
                     /gene="atpI"
                     /locus_tag="KJL99_003525"
     CDS             342..722
                     /gene="atpI"
                     /locus_tag="KJL99_003525"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HBD4438118.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            731..1546
                     /gene="atpB"
                     /locus_tag="KJL99_003526"
     CDS             731..1546
                     /gene="atpB"
                     /locus_tag="KJL99_003526"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HBD4438119.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYNIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            1593..1832
                     /gene="atpE"
                     /locus_tag="KJL99_003527"
     CDS             1593..1832
                     /gene="atpE"
                     /locus_tag="KJL99_003527"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HBD4438120.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            1894..2364
                     /gene="atpF"
                     /locus_tag="KJL99_003528"
     CDS             1894..2364
                     /gene="atpF"
                     /locus_tag="KJL99_003528"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HBD4438121.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            2379..2912
                     /gene="atpH"
                     /locus_tag="KJL99_003529"
     CDS             2379..2912
                     /gene="atpH"
                     /locus_tag="KJL99_003529"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HBD4438122.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLTFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRSGDMVI
                     DGSVRGRLERLADVLQS"
     gene            2925..4466
                     /gene="atpA"
                     /locus_tag="KJL99_003530"
     CDS             2925..4466
                     /gene="atpA"
                     /locus_tag="KJL99_003530"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HBD4438123.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            4517..5380
                     /gene="atpG"
                     /locus_tag="KJL99_003531"
     CDS             4517..5380
                     /gene="atpG"
                     /locus_tag="KJL99_003531"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HBD4438124.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            5407..6789
                     /gene="atpD"
                     /locus_tag="KJL99_003532"
     CDS             5407..6789
                     /gene="atpD"
                     /locus_tag="KJL99_003532"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HBD4438125.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            6810..7229
                     /gene="atpC"
                     /locus_tag="KJL99_003533"
     CDS             6810..7229
                     /gene="atpC"
                     /locus_tag="KJL99_003533"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HBD4438126.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            7580..8950
                     /gene="glmU"
                     /locus_tag="KJL99_003534"
     CDS             7580..8950
                     /gene="glmU"
                     /locus_tag="KJL99_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HBD4438127.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGTKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            9112..10941
                     /gene="glmS"
                     /locus_tag="KJL99_003535"
     CDS             9112..10941
                     /gene="glmS"
                     /locus_tag="KJL99_003535"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HBD4438128.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
BASE COUNT         2636 a         2728 c         3000 g         2606 t
ORIGIN      
        1 tttttagact gaaatatcat aaacttgtaa aggcatcatt tgccaagtaa ataaatatgc
       61 tgtgcgcgaa catgcgcaat atgtgatctg aagcacgctt tatcaccagt gtttacgcgt
      121 tatttacagt ttttcatgat cgaacagggg tagcggaaaa gtcgcaattg tatgcactgg
      181 aaaaatattt aaacatttat tcaccttttg gctacttatt gtttgaaatc acgggggcgc
      241 accgtataat ttgaccgctt tttgatgctt gactctaagc cttaaagaaa gttttatacg
      301 acacgcggca tacctcgaag ggagcaggag tgaaaaacgt gatgtctgtg tcgctcgtga
      361 gtcgaaacgt tgctcggaag cttctgctcg ttcagttact ggtggtgata gcaagtggat
      421 tgctgttcag cctcaaagac cccttctggg gcgtctctgc aataagcggg ggcctggcag
      481 tctttctgcc taacgttttg tttatgatat ttgcctggcg tcaccaggcg catacaccag
      541 cgaaaggccg ggtggcttgg acattcgcat ttggcgaagc tttcaaagtt ctggcgatgt
      601 tggtgttact ggtggtggcg ttggcggttt taaaggcggt attcttgccg ctgatcgtta
      661 cgtgggtttt ggtgctggtg gttcagatac tggcaccggc tgtaattaac aacaaagggt
      721 aaaaggcatc atggcttcag aaaatatgac gccgcaggat tacataggac accacctgaa
      781 taaccttcag ctggacctgc gtacattctc gctggtggat ccacaaaacc ccccagccac
      841 cttctggaca atcaatattg actccatgtt cttctcggtg gtgctgggtc tgttgttcct
      901 ggttttattc cgtagcgtag ccaaaaaggc gaccagcggt gtgccaggta agtttcagac
      961 cgcgattgag ctggtgatcg gctttgttaa tggtagcgtg aaagacatgt accatggcaa
     1021 aagcaagctg attgctccgc tggccctgac gatcttcgtc tgggtattcc tgatgaatct
     1081 gatggattta ctgcctatcg acttgctgcc gtacattgct gaacatgtac tgggtctgcc
     1141 tgcactgcgt gtggttccgt ctgcggacgt gaacgtaacg ctgtctatgg cactgggcgt
     1201 atttatcctg attctgttct acaacatcaa aatgaaaggc atcggcggct tcacgaaaga
     1261 gttgacgctg cagccgttca atcactgggc gttcattcct gtcaacttaa tccttgaagg
     1321 ggtaagcctg ctgtccaaac cagtttcact cggtttgcga ctgttcggta acatgtatgc
     1381 cggtgagctg attttcattc tgattgctgg tctgttgccg tggtggtcac agtggatcct
     1441 gaatgtgccg tgggccattt tccacatcct gatcattacg ctgcaagcct tcatcttcat
     1501 ggttctgacg atcgtctatc tgtcgatggc gtctgaagaa cattaattta ccaacactac
     1561 tacgttttaa ctgaaacaaa ctggagactg tcatggaaaa cctgaatatg gatctgctgt
     1621 acatggctgc cgctgtgatg atgggtctgg cggcaatcgg tgctgcgatc ggtatcggca
     1681 tcctcggggg taaattcctg gaaggcgcag cgcgtcaacc tgatctgatt cctctgctgc
     1741 gtactcagtt ctttatcgtt atgggtctgg tggatgctat cccgatgatc gctgtaggtc
     1801 tgggtctgta cgtgatgttc gctgtcgcgt agtaagcgtt gcttttattt aaagagcaat
     1861 atcagaacgt taactaaata gaggcattgt gctgtgaatc ttaacgcaac aatcctcggc
     1921 caggccatcg cgtttgtcct gttcgttctg ttctgcatga agtacgtatg gccgccatta
     1981 atggcagcca tcgaaaaacg tcaaaaagaa attgctgacg gccttgcttc cgcagaacga
     2041 gcacataagg accttgacct tgcaaaggcc agcgcgaccg accagctgaa aaaagcgaaa
     2101 gcggaagccc aggtaatcat cgagcaggcg aacaaacgcc gctcgcagat tctggacgaa
     2161 gcgaaagctg aggcagaaca ggaacgtact aaaatcgtgg cccaggcgca ggcagaaatt
     2221 gaagccgagc gtaaacgtgc ccgtgaagag ctgcgtaagc aagttgctat cctggctgtt
     2281 gctggcgccg agaagatcat cgaacgttcc gtggatgaag ctgctaacag cgacatcgtg
     2341 gataaacttg tcgctgaact gtaaggaggg aggggctgat gtctgaattt attacggtag
     2401 ctcgccccta cgccaaagca gcttttgact ttgccgtcga acaccaaagt gtagaacgct
     2461 ggcaggacat gctgacgttt gccgccgagg taaccaaaaa cgaacaaatg gcagagcttc
     2521 tctctggcgc gcttgcgcca gaaacgctcg ccgagtcgtt tatcgcagtt tgtggtgagc
     2581 aactggacga aaacggtcag aacctgattc gggtaatggc tgaaaatggt cgtcttaacg
     2641 cgctcccgga tgttctggag cagtttattc accttcgtgc cgtgagtgag gctaccgctg
     2701 aggtagacgt catttccgct gccgcactga gtgaacaaca gctcgcgaaa atttctgctg
     2761 cgatggaaaa acgtctgtca cgcaaagtta agctgaattg caaaatcgat aagtctgtaa
     2821 tggctggcgt tatcatccga tcgggtgata tggtcattga tggcagcgta cgcggtcgtc
     2881 ttgagcgcct tgcagacgtc ttgcagtctt aaggggactg gagcatgcaa ctgaattcca
     2941 ccgaaatcag cgaactgatc aagcagcgca ttgctcagtt caatgttgtg agtgaagctc
     3001 acaacgaagg tactattgtt tctgtaagtg acggtgttat ccgcattcac ggcctggccg
     3061 attgtatgca gggtgaaatg atctccctgc cgggtaaccg ttacgctatc gcactgaacc
     3121 tcgagcgcga ctctgtaggt gcggttgtta tgggtccgta cgctgacctt gccgaaggca
     3181 tgaaagttaa gtgtactggc cgtatcctgg aagttccggt tggccgtggc ctgctgggcc
     3241 gtgtggttaa cactctgggt gcaccaatcg acggtaaagg tccgctggat cacgacggct
     3301 tctctgctgt agaagcaatc gctccgggcg ttatcgaacg tcagtccgta gatcagccgg
     3361 tacagaccgg ttataaagcc gttgactcca tgatcccaat cggtcgtggt cagcgtgaat
     3421 tgatcatcgg tgaccgtcag acaggtaaaa ccgcactggc tatcgatgcc atcatcaacc
     3481 agcgcgattc cggtatcaaa tgtatctatg tcgctatcgg ccagaaagcg tccaccattt
     3541 ctaacgtggt acgtaaactg gaagagcacg gcgcactggc taacaccatc gttgtggtag
     3601 caaccgcgtc tgaatccgct gcactgcaat acctggcacc gtatgccggt tgcgcaatgg
     3661 gcgaatactt ccgtgaccgc ggtgaagatg cgctgatcat ttacgatgac ctgtctaaac
     3721 aggctgttgc ttaccgtcag atctccctgc tgctccgtcg tccgccagga cgtgaagcat
     3781 tcccgggcga cgttttctac ctccactctc gtctgctgga gcgtgctgca cgtgttaacg
     3841 ccgaatacgt tgaagccttc accaaaggtg aagtgaaagg gaaaaccggt tctctgaccg
     3901 cactgccgat tatcgaaact caggcgggtg acgtttctgc gttcgttccg accaacgtaa
     3961 tctccattac cgatggtcag atcttcctgg aaaccaacct gttcaacgcc ggtattcgtc
     4021 ctgcggttaa cccgggtatt tccgtatccc gtgttggtgg tgcagcacag accaagatca
     4081 tgaaaaaact gtccggtggt atccgtaccg ctctggcaca gtatcgtgaa ctggcagcgt
     4141 tctctcagtt tgcatccgac cttgacgatg caacacgtaa gcagcttgac cacggtcaga
     4201 aagtgaccga actgctgaaa cagaaacagt atgcgccgat gtccgttgcg cagcagtctc
     4261 tggttctgtt cgcagcagaa cgtggttacc tggcggatgt tgaactgtcg aaaattggca
     4321 gcttcgaagc cgctctgctg gcttacgtcg accgtgatca cgctccgttg atgcaagaga
     4381 tcaaccagac cggtggctac aacgacgaaa tcgaaggcaa gctgaaaggc atcctcgatt
     4441 ccttcaaagc aacccaatcc tggtaacgtc tggaggcttg ccttagggca ggccgcaagg
     4501 cattgaggag aagctcatgg ccggcgcaaa agagatacgt agtaagatcg caagcgtcca
     4561 gaacacgcaa aagatcacta aagcgatgga gatggtcgcc gcttccaaaa tgcgtaaatc
     4621 gcaggatcgc atggcggcca gccgtcctta tgcagaaacc atgcgcaaag tgattggtca
     4681 ccttgcacac ggtaatctgg aatataagca cccttacctg gaagaccgcg acgttaaacg
     4741 cgtgggctac ctggtggtgt cgaccgaccg tggtttgtgc ggtggtttga acattaacct
     4801 gttcaaaaaa ctgctggcgg aaatgaagac ctggaccgac aaaggcgttc aatgcgacct
     4861 cgcaatgatc ggctcgaaag gcgtgtcgtt cttcaactcc gtgggcggca atgttgttgc
     4921 ccaggtcacc ggcatggggg ataacccttc cctgtccgaa ctgatcggtc cggtaaaagt
     4981 gatgttgcag gcctacgacg aaggccgtct ggacaagctt tacattgtca gcaacaaatt
     5041 tattaacacc atgtctcagg ttccgaccat cagccagctg ctgccgttac cggcatcaga
     5101 tgatgatgat ctgaaacata aatcctggga ttacctgtac gaacccgatc cgaaggcgtt
     5161 gctggatacc ctgctgcgtc gttatgtcga atctcaggtt tatcagggcg tggttgaaaa
     5221 cctggccagc gagcaggccg cccgtatggt ggcgatgaaa gccgcgaccg acaatggcgg
     5281 cagcctgatt aaagagctgc agttggtata caacaaagct cgtcaggcca gcattactca
     5341 ggaactcacc gagatcgtct cgggggccgc cgcggtttaa acaggttatt tcgtagagga
     5401 tttaagatgg ctactggaaa gattgtccag gtaatcggcg ccgtagttga cgtcgaattc
     5461 cctcaggatg ccgtaccgcg cgtgtacgat gctcttgagg tgcaaaatgg taatgagcgt
     5521 ctggtgctgg aagttcagca gcagctcggc ggcggtatcg tgcgtaccat cgcaatgggt
     5581 tcctccgacg gtctgcgtcg cggtctggat gtaaaagacc tcgaacaccc gatcgaagtc
     5641 ccggtaggta aagcgactct gggccgtatc atgaacgtac tgggtgaacc ggtcgacatg
     5701 aaaggcgaga tcggtgaaga agagcgttgg gcgattcacc gcgcagcacc ttcctacgaa
     5761 gagctgtcaa actctcagga actgctggaa accggtatca aagttatcga cctgatgtgt
     5821 ccgttcgcta agggcggtaa agttggtctg ttcggtggtg cgggtgtagg taaaaccgta
     5881 aacatgatgg agctcattcg taacatcgcg atcgagcact ccggttactc tgtgtttgcg
     5941 ggcgtaggtg aacgtactcg tgagggtaac gacttctacc acgaaatgac cgactccaac
     6001 gttatcgaca aagtatccct ggtgtatggc cagatgaacg agccgccggg aaaccgtctg
     6061 cgcgttgctc tgaccggtct gaccatggct gagaaattcc gtgacgaagg tcgtgacgtt
     6121 ctgctgttcg ttgacaacat ctatcgttac accctggccg gtacggaagt atccgcactg
     6181 ctgggccgta tgccttcagc ggtaggttat cagccgaccc tggcggaaga gatgggcgtt
     6241 ctgcaggaac gtatcacctc caccaaaact ggttctatca cctccgtaca ggcggtatac
     6301 gtacctgcgg atgacttgac tgacccgtct ccggcaacca cctttgcgca ccttgacgca
     6361 accgtggtac tgagccgtca gatcgcgtct ctgggtatct acccggccgt tgacccgctg
     6421 gactccacca gccgtcagct ggacccgctg gtggttggtc aggaacacta cgacaccgcg
     6481 cgtggcgttc agtccatcct gcaacgttat caggaactga aagacatcat cgccatcctg
     6541 ggtatggatg aactgtctga agaagacaaa ctggtggtag cgcgtgctcg taagatccag
     6601 cgcttcctgt cccagccgtt cttcgtggca gaagtattca ccggttctcc gggtaaatac
     6661 gtctccctga aagacaccat ccgtggcttt aaaggcatca tggaaggcga atacgatcac
     6721 ctgccggagc aggcgttcta catggtcggt tccatcgaag aagctgtgga aaaagccaaa
     6781 aaactttaac gccttaatcg gagggtgata tggcaatgac ttaccacctg gacgtcgtca
     6841 gcgcagagca acaaatgttc tctggtctgg tcgagaaaat ccaggtaacg ggtagcgaag
     6901 gtgaactggg gatctaccct ggccacgcac cgctgctcac cgccattaag cctggtatga
     6961 ttcgcatcgt gaaacagcac ggtcacgaag agtttatcta tctgtctggc ggcattcttg
     7021 aagtgcagcc tggcaacgtg accgttctgg ccgacaccgc aattcgcggc caggatctcg
     7081 acgaagcgcg agccatggaa gcgaaacgta aggctgaaga gcacattagc agctctcacg
     7141 gcgacgtaga ttacgctcag gcgtctgcgg aactggccaa agcgatcgcg cagctgcgcg
     7201 ttatcgagtt gaccaaaaaa gcgatgtaac accggcttga aaagcacaaa agccagtctg
     7261 gaaacaggct ggcttttttt acgcgttgac ccgtcctgaa gagcgttcac atagatcctg
     7321 ctgatataaa acacccctgt tttcccgttt attcattgat cgaaataaga gcaaaaacat
     7381 ccacctgacg cttaaatgaa ggtactgcct taattttctg cagataaaag gcgtgacgat
     7441 ggtcgaaaat ggcactttcg tcagcgggga taatccgtta ttgaacaatt tatcctctgt
     7501 ccatttcacg atgaaaaaaa tgtagttttt tcaaggtgaa gcggtttaaa ttcgttctca
     7561 aattacagtc aggacgcgta tgttgaataa tgctatgagc gtagtgatcc ttgccgcagg
     7621 caaaggcacg cgcatgtatt ccgatcttcc gaaagtgctg catacccttg ccgggaaagc
     7681 gatggttcag catgtcattg atgctgcgaa tgaattaggc gcagcgcacg ttcacctggt
     7741 gtacggtcac ggcggcgatc tgcttaaaca ggcgctgaaa gacgacaacc ttaactgggt
     7801 gcttcaggca gagcagctgg gtacgggtca tgcaatgcag caggccgcac ctttctttgc
     7861 cgatgatgaa gacattttaa tgctctacgg cgacgtgccg ctgatctctg tcgaaacact
     7921 ccagcgcctg cgtgatgcta aaccccaggg tggcattggt ctgctgacgg tgaaactgga
     7981 tgatccgacc ggttatggac gtatcacccg tgaaaatggc aaagttaccg gcattgttga
     8041 gcacaaagat gccaccgacg agcagcgtca gattcaggag atcaacaccg gcattctgat
     8101 tgccaacggc gcagatatga aacgctggct ggcgaagctg accaacaata atgctcaggg
     8161 cgaatactac atcaccgaca ttattgcgct ggcgtatcag gaagggcgtg aaatcgtcgc
     8221 cgttcatccg caacgtttaa gcgaagtaga aggtgtgaat aaccgcctgc aactctcccg
     8281 tctggagcgc gtttaccagt ccgaacaggc tgaaaaactg ctgttagcag gcgttatgct
     8341 gcgcgatccg gcgcgttttg atctgcgcgg tacgcttact cacgggcgcg atgttgaaat
     8401 tgatactaac gttatcatcg agggcaacgt gactctcggt catcgcgtga aaatcggcac
     8461 cggttgcgtg attaaaaaca gcgtgattgg cgatgattgc gaaatcagtc cgtataccgt
     8521 tgtggaagac gcgaatctgg cagcggcctg tactattggc ccgtttgccc gtctgcgtcc
     8581 tggtgctgag ttgctggaag gtgcacacgt tggtaacttc gttgagatga aaaaagcgcg
     8641 tctgggtaaa ggcacgaaag ccgggcatct gacttacctg ggcgatgcgg aaattggcga
     8701 taacgttaac atcggcgcgg gaaccattac ctgcaactac gatggtgcga ataaatttaa
     8761 gaccattatc ggcgacgatg tgtttgtcgg ttccgacact cagctggtgg ccccggtaac
     8821 agtaggcaaa ggcgcgacca ttgctgcggg tacaactgtg acgcgtaatg tcggcgaaaa
     8881 tgcattagct atcagccgtg tgccgcagac tcagaaagaa ggctggcgtc gtccggtaaa
     8941 gaaaaagtaa ttctggccgg gtaatccggt catctgggat gaggagataa cataatctcc
     9001 ctcccacaag cagtaactat aaaaataacc ccactctcta caaggctcgg ggcgcccgaa
     9061 aaaacgggca tacaggttga ccgacaacga tataaatcgg aatcaaaaac tatgtgtgga
     9121 attgttggcg cgatcgcgca acgtgatgta gcagaaatcc ttcttgaagg tttacgtcgt
     9181 ctggaatacc gcggatatga ctctgccggt ctggccgttg ttgatgcgga aggtcatatg
     9241 actcgcctgc gtcgcctcgg taaagtccag atgctggctc aggcagcgga agaacatcct
     9301 ctgcatggcg gcaccggtat tgctcacact cgctgggcga cccacggtga accttcagaa
     9361 gctaacgcgc atccgcatgt ttctgaacac attgtggtgg tgcataatgg catcatcgaa
     9421 aaccatgaac cgctgcgtga agagttaaaa gcgcgtggct ataccttcgt ttctgaaacc
     9481 gacaccgaag tgattgccca tctggtgaac tgggagctga aacaaggcgg gactctgcgt
     9541 gaggctgttc tgcgtgctat cccgcagctg cgcggtgcgt acggtacagt gatcatggac
     9601 tcccgtcacc cggataccct gctagcggca cgttctggta gtccgctggt gattggcctg
     9661 ggtatgggcg aaaactttat cgcttctgac cagctggcgc tgttgccggt gacccgtcgc
     9721 tttatcttcc ttgaagaggg cgatattgcg gaaatcactc gccgttcggt aaacatcttc
     9781 gataaaactg gcgcggaagt aaaacgtcag gatatcgaat ccaatctgca atatgacgcg
     9841 ggcgataaag gtatttaccg tcactacatg cagaaagaga tctacgaaca gccgaacgcg
     9901 ataaaaaaca cccttaccgg gcgcatcagc cacggtcagg ttgatttaag cgagctggga
     9961 ccgaacgccg acgaactgct gtcgaaggtt gagcatattc agatcctcgc ctgtggtact
    10021 tcttataact ccggtatggt ttcccgctac tggtttgaat cgctggcagg tattccgtgc
    10081 gacgtcgaaa tcgcctctga attccgctat cgtaaatctg ccgtgcgtcg taacagcctg
    10141 atgatcacct tgtcacagtc tggcgaaacc gcggataccc tggcgggcct gcgtctgtcg
    10201 aaagagctgg gttaccttgg ttcgctggca atctgtaacg ttccgggttc ttctctggtg
    10261 cgcgaatccg atctggcgct gatgaccaac gcgggtacgg aaatcggcgt ggcgtccact
    10321 aaagcattca ccactcagtt aactgtgctg ttgatgctgg tggcgaagct gtctcgcctg
    10381 aaaggtctgg atgcctccat tgaacatgac attgtgcatg gtctgcaggc gctgccgagc
    10441 cgtattgagc agatgctgtc tcaggataaa cgcattgaag ctctggcaga agatttctct
    10501 gacaaacatc acgcgctgtt cctgggccgt ggcgatcagt acccaatcgc gctggaaggc
    10561 gcattgaagc tgaaagagat ctcttacatt cacgctgaag cctacgctgc aggcgagctg
    10621 aaacacggcc cgctggcgct gattgacgcg gatatgccgg ttatcgtcgt tgcaccgaac
    10681 aacgaactgt tggaaaaact gaaatccaac attgaagaag ttcgcgcgcg tggcggtcaa
    10741 ttgtatgtct tcgccgatca ggatgcgggt tttgtaagta gcgataacat gcacatcatc
    10801 gagatgccgc atgtggaaga ggtgattgca ccgatcttct acaccgttcc gctgcagctg
    10861 ctggcttacc atgtcgcgct gatcaaaggc accgacgttg accagccgcg taacctggca
    10921 aaatcggtta cggttgagta atcactatca tagataaaaa agggctgccg
//
LOCUS       DAEBXN010000126        10926 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.126, whole genome shotgun sequence.
ACCESSION   DAEBXN010000126 DAEBXN010000000
VERSION     DAEBXN010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10926
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.126"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            154..504
                     /locus_tag="KJL99_003536"
     CDS             154..504
                     /locus_tag="KJL99_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HBD4438129.1"
                     /translation="MLILDSRPVHASRPHSEAIRDAQRKKPKVPVHAVLTATNPLIRF
                     IGSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPE
                     IGAVPAIPQQSSLF"
     gene            557..952
                     /locus_tag="KJL99_003537"
     CDS             557..952
                     /locus_tag="KJL99_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HBD4438130.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     CIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            993..1736
                     /gene="cmoA"
                     /locus_tag="KJL99_003538"
     CDS             993..1736
                     /gene="cmoA"
                     /locus_tag="KJL99_003538"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HBD4438131.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            1733..2704
                     /gene="cmoB"
                     /locus_tag="KJL99_003539"
     CDS             1733..2704
                     /gene="cmoB"
                     /locus_tag="KJL99_003539"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HBD4438132.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     TVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(2869..5298)
                     /gene="torZ"
                     /locus_tag="KJL99_003540"
     CDS             complement(2869..5298)
                     /gene="torZ"
                     /locus_tag="KJL99_003540"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HBD4438133.1"
                     /translation="MALTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQASWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWTAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQRANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(5323..6423)
                     /locus_tag="KJL99_003541"
     CDS             complement(5323..6423)
                     /locus_tag="KJL99_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HBD4438134.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASIAADHYTVNAWPSIA
                     KGMGARTSMSENEPDILTRYFQYNAKDITEKQ"
     gene            complement(6811..7557)
                     /gene="cutC"
                     /locus_tag="KJL99_003542"
     CDS             complement(6811..7557)
                     /gene="cutC"
                     /locus_tag="KJL99_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HBD4438135.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(7571..8137)
                     /locus_tag="KJL99_003543"
     CDS             complement(7571..8137)
                     /locus_tag="KJL99_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBD4438136.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSEKMINGRPICLFKLHEPVQVAHWQFSNVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            8353..10086
                     /gene="argS"
                     /locus_tag="KJL99_003544"
     CDS             8353..10086
                     /gene="argS"
                     /locus_tag="KJL99_003544"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HBD4438137.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQIRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDTAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDVFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            10263..10751
                     /locus_tag="KJL99_003545"
     CDS             10263..10751
                     /locus_tag="KJL99_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HBD4438138.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDTSAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGAKKLDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
BASE COUNT         2669 a         2788 c         2605 g         2864 t
ORIGIN      
        1 taatgggctt ttaatcctat cgtttattgc ttgcatagta gaatctaacc atccatcact
       61 catagctaac cctcggaaat cttacatttc tgtctatcat aaccactagc atcaccttta
      121 ggaaccatct gaaacgggag gtgtatttag accttgctaa tcttagacag ccgcccggtt
      181 catgcatcac gtccacacag tgaagctatt cgcgacgctc aacgaaaaaa acctaaagtt
      241 ccggtacatg ctgtactgac ggcgacaaat ccgctgatcc gttttatcgg tagtgatgat
      301 atgacgcaaa accgggaatt atttcaggtc tggttacaaa aattagcgca gtggcatcag
      361 accactacgc cttatctttt tttacatacg ccagatattg cccaggcccc ggaactggta
      421 cataccctgt gggaagactt acgtaaaacg cttccagaga tcggagcagt tccggctatt
      481 ccacagcaat cttctctttt ctgaatttgc cacctatcat agacaggtgc catcggccat
      541 tttaaaggga gtttgtatgg taagcgcgct atatgccgtt ttaagtgcgt tgttattaat
      601 gaagttctct tttgatgtcg ttcgcctgcg aatgcagtac cgcgttgcct atggtgacgg
      661 tggttttagc gaactgcaaa gcgctatttg cattcatggt aacgcggtgg aatatattcc
      721 catcgcgatt gtgctgatgc tgtttatgga aatgaatggc gcagaaacct ggatggtgca
      781 tatttgcggc atcgttttgc ttgctggtcg tctgatgcat tattacggtt ttcatcaccg
      841 tctgtttcgc tggcgacgtt ccggcatgag cgccacctgg tgtgcgctgt tgctgatggt
      901 gctggcgaat ctttggtata tgccctggga gttggttttc tccctgcgtt agcgcacaat
      961 acgccacttt ctttttcccg gatttttacg ttatgtctca ccgcgacacg ctattttctg
     1021 cccctatcgc cagactgggc gactggacct ttgatgaacg ggtcgctgaa gtcttcccgg
     1081 atatgatcca gcgttccgtt cccggctatt ccaatattat ttccatgatt ggtatgttag
     1141 ccgagcgctt cgttcaacct ggtacgcagg tttacgatct gggttgttct ctgggcgcgg
     1201 cgacgctctc ggtgcgtcgc aacattcatc atgataattg caaaattatt gccatcgaca
     1261 actccccggc gatgattgaa cgctgccgtc gtcatattga cgcctataaa gcccctacgc
     1321 cagtagacgt cattgaaggt gatattcgcg atatcgccat tgaaaacgca tcgatggtgg
     1381 tgctgaattt taccctgcaa ttcctggaac cttccgagcg ccaggcatta ctggataaaa
     1441 tttatcaagg actgaacccg ggcggcgcgc tggtgctttc ggaaaaattc agtttcgaag
     1501 atgccaaagt tggtgaactg ttgttcaaca tgcaccacga ctttaaacgt gccaacggtt
     1561 acagcgaact ggagatcagc cagaagcgca gcatgctgga aaacgtgatg ctgaccgatt
     1621 ccgtggaaac ccataaagca cgcctgcata aagccggttt tgagcatagc gagctgtggt
     1681 tccagtgctt taactttggt tcactggtgg cattaaaagc agaggacgct gcatgatcga
     1741 ctttggtaac ttttattctc tgattgccaa aaatcatctt tcacactggc tcgaaacgct
     1801 gcccgcgcag attgctaact ggcagcgcga gcagcagcac gggctgttta agcagtggtc
     1861 caacacggtg gaatttctgc ctgaaattaa accgtatcgt ctggatttat tgcatagcgt
     1921 aaccgccgaa agcgaagagc cactgagcac cgggcaaatt aaacgcattg aaacgcttat
     1981 gcggaacctg atgccatggc gcaaagggcc gttctcactg tatggcgtca acatcgatac
     2041 cgaatggcgt tccgactgga aatgggatcg tgttctgccc catctttctg atttaaccgg
     2101 acgcaccatt ctggatgtcg gctgcggcag cggttatcac atgtggcgca tgattggcgc
     2161 aggcgcgcac ctcgcggtgg ggatcgaccc tacgcaactg tttctgtgcc agtttgaagc
     2221 ggtgcgtaaa ctactgggta acgatcagcg cgcacatttg ttaccgttag gtattgaaca
     2281 gcttccggca ctgaaagcct ttgataccgt cttttcgatg ggcgtgcttt accatcgccg
     2341 ttcgccgctg gagcatctct ggcagttgaa agatcaactg gtgaatgagg gtgaactggt
     2401 gctggaaacg ctggttattg atggcgatga gaacacggtg ctggtaccag gcgatcgtta
     2461 cgcgcaaatg cgtaatgtct atttcatccc ttccgcgctg gcgctgaaaa actggctgaa
     2521 gaagtgtggt tttgtagata tccgcattgt ggatgtgtgc gttaccacta ctgaagagca
     2581 gcgacgcact gaatggatgg ttaccgaatc tctgtccgat ttcctcgacc cacatgatcc
     2641 gagtaagaca gttgaaggtt atcctgcgcc taaacgcgcg gtgctgattg cgcgcaagcc
     2701 gtaaaggtct ggtattactg ccggatgcgg cgtgaacgcc ttatccggcc tacaaaccct
     2761 tgctaattca atctattgca gggactatgt aggcctgata agcatagcgc atcaggcagt
     2821 tttacgtttg cataacctca gcgcccgttt ccgggcgcta ttcacttctt acgcctgaac
     2881 agcaggctta tcaaatgccg ttaacttcgg tgcgttaccc gtatattttt cgatatacac
     2941 cagcgcagag ttaccggcac aggcatttgc ccgctgcgag ctggggatat ccgccgttaa
     3001 cacgttcgca ctgccgtttt tacacaagcc gttttccaga tctggccatg caccttcatg
     3061 cacgcatacc acgccttttt tgatcccgtc agtcaccacc gcgcctgtca aaatctgacc
     3121 acgtttgttc cagacacgca ccagatcgcc attcgcaata ccaaatcgag cagcatcttc
     3181 ggtgtgaata gtgatcggtt cacgatcggc aaccgcatat tttttacgca gttccgcata
     3241 gttaagctga ctgtgtaaac ggtgtgccgg atgtgcggtc agaagctgca actgcttctc
     3301 gtcggcggta cccttccact catcaggcgc aagccaggtt gggtgtgccg ggcaatcctt
     3361 atagccaaat ttttccagcg ttttggagta aatctcaatt ttgccgcttg gcgtacccag
     3421 cgcatttttc accggatcgg cgcggaaatc accataacga acgtactgtt cgttcttctc
     3481 gctgcggcgc atttcgatca gtttattttg ctgccagaag gcattaaaca ttggcatagt
     3541 gacgcgttgc gcacgggccc ctttctgagc ggcatcatag aaaaatttca gccacgccat
     3601 ttcatcttta ccttcggtat agatctcttt tccgccaggt ttgagtaatt ccgcaagatc
     3661 ggcaaacacg tcaaaatcgt tacgcgcttc aaattgcgga gcaacagcct gcttcatcgg
     3721 cacaatatgc tggttgctgt aatcaccggt catcgtcaag tcattgcgct caaacgatgt
     3781 ggtgatcggt aatacgatat ctgcgtgttt ggctgctgcg gtccagtagc attcagaaac
     3841 gacgatcatc tccggtttct gccaggcttt gatcagacga ttagtatcct ggtgatgggt
     3901 aaagttaccg ccacccgccc accagatcat tttgatattc ggatacgttt gttctttacc
     3961 gttatgctga tatttgccgc caggattttc cagcgcatcg acgatacggg caacaggaat
     4021 cgccgtcatt ccgccatcat cagcggcctc gctggcctgt ccggcaatcg ccgcagacat
     4081 ttccggcagc acgccgccaa cacgcgtcgg gttgccacca ttggagtaat gataagagaa
     4141 accaaaaccg ccgcccggtg taccaatctg ccctaacatc gccgccagcg tcaccagcat
     4201 ccagtgtttc tgttcaccat attgctggcg ctgaattccc cagcctgcca tcagcattgt
     4261 gcgattcgcc gccatcagtt cagcgagttt gactatctgc gcttccggta cgccagtaat
     4321 ttccgccgtc cagaccgcgc tcttcggcgt attatcgctc ttacctgtca gatactcttc
     4381 aaactgcgga tacccggtag tgtatttttc aaggaacact ttatcgtgtt tgccttgtgt
     4441 catcagggta tgcgcaatcc ctaacatcag tgccacgtcg gtgcccatat tcggcgcgat
     4501 ccaggtggca ttatcgccaa agaattcgat ggtttcggag cggataggat caatggcaat
     4561 cactggtttg ccagattttt tcagctgatg gaagtattcc agcccttgct catcggtact
     4621 gctccaggca atttttaagg tattcagcgg gttcattccc cacagcacca caacctggct
     4681 gttttccaga atcagcggcc aggaggcctg ctgttcatac acctctacag aaccgaccac
     4741 atgcggcatg atcacctgtg ctgccccggt tgaataatcg ccgctatgcc cggaataacc
     4801 gcccgccagg ttcatataac gttgcagtaa ggtttgcgct ttatgcaaca cgccagaaga
     4861 gcgccagccg taagatccgg caaaaatggc cgatggtccg ttagctttac gaatacgctc
     4921 atgttgctca tgaatcagtt ttaatgcctg ttcccagctc acctgtacat aggtatcttc
     4981 gccacgacct ttcgccggtt gcagtggatt gtcgagatag ctttttctca ccatcggatg
     5041 ctgaatacgc gccgtggtgt gtacctgatc cgccgccgta gactgtaagg agttcggtat
     5101 ggttttcgcc agcgcgcctg tcgaagaaac aatcttgccg tccttcactt ctacgctcat
     5161 cgctccccaa cgtcccgcgg tgaggatttt accgcccttc tcttctgccc atgcggggag
     5221 cggtgctgcc gatgtcacca ccagcgctcc agcggcaata ccgctgtgtt taataaattc
     5281 acgtcttgtt aatgccataa cttcctccct gatcaacgag gatcactgtt tctcggtaat
     5341 atctttggcg ttgtactgga aataccgcgt taaaatgtcc ggttcgtttt cgctcatgct
     5401 ggttcgtgcc cccattcctt tggcaatgga cggccacgca ttgacggtgt aatggtcggc
     5461 ggcaatagag gcatgacaac cagcgcaata ggtatcgtca agtttttcag cgtattgcca
     5521 tagcggttta cggtccgcta atgcgggatc ggtaagcgca ccctgtaaag acgcctgacg
     5581 ccattgattg ccgtattcgt cagcctgcca ttccccgttt accgtgagcg ccttgatacc
     5641 ttcttcactt aatgtggcta gcgccagccg ttgacctgcc gccaggtaga gcgtgttttc
     5701 actgccctgc atttgataac cctgcaacag aacgatcggc tgtttgccac tggcatcaac
     5761 gacggtgaga tcggtaccag gattcacggt agccagctcg cctatgcgag aagttttgaa
     5821 aggataaata tgtgcgccat tagtcactga agtagcggcc tgactttcca gctcatgcgc
     5881 cgcgttgtca tccattttta tttctggcgg aaaatgggca atgcctttat gacaatcgat
     5941 acaggtttcg ccgccctttt gtgctttgtt atgcattttc tgcgcagatt cactttgcga
     6001 ggcaatatcc atggcatcaa aagaatggca actacggcac gttgcagagt cagttgcttt
     6061 taattctttc catactgttt cggccatttc ctggcgatga gtttcgaact tatcgtcact
     6121 gtctattttg ccgctaacaa attcatgata aatatcttta gatgctttta atttagcaaa
     6181 taaataatcc atccctgact ttggaatatg gcaatcggca cattccgcac gtatcccttt
     6241 ctggttcgaa aagtggacag ttccctgata ttcctcaaaa ggtttactca tcgagtggca
     6301 ggaaaggcaa aatgctgtat ccgacgtttt atgtaaggct ttttgcgcca gcaataaccc
     6361 gccgccacca accacaaccg ctatcagcaa aaacaataac ccaatgcgtt ttttccctcg
     6421 catagattcc tctaaataaa ttccagaaca ccgaaatcat tgtataatta aatacacaac
     6481 gaattaaccg cttgcataac tcggcacaac actgcctgaa ataatcgata aagaatatga
     6541 tttattacaa tgtaatcatt aattgctaag gaataaccca gttgctattt agaatatata
     6601 tttatcagtt ttagcaattt aaatcccata atcaatgtga atataaaaaa tatttttagc
     6661 acaacgctat gcgcactgtc acccgctcaa cagatcacgg ttatcgttcg ttttttatac
     6721 tgctcaggga taaaaaaagg cccctgttga aattgcaggg gcctggtacg agcaagcatc
     6781 atattgggcg acatgatgca acggtaaaaa tcatttggcc tgatggcgtt caatgattcc
     6841 tttcatttca gcaaccgccg ccccgtctac gatataacgc gaatactcgt ccgcgtgttc
     6901 atctgatgac atggacaatc cttgattacg ataacgcatc ggtgaggctt gccacgctcc
     6961 cgcggagcta tggacttcca gcactccggc atcgaggaag tggtgcaggt tttctgcacg
     7021 gactcctgct ccagccataa tgattggagc atcacgatgg gcaataagtt ccataatttt
     7081 tgataaacct tgcagtgcgt ctgatttttg ccctgatgtc agtacccggg caatgcccaa
     7141 ttccgcaaga ttattgagtg tatataaagg gttagcgcac atatcgaagg cgcgatgaaa
     7201 agtcactgcc agcggaccgg cagcagccat tattttttcc attcgtggca tatcgacatt
     7261 cccgtcaaca tcgagaacgc ccgtcaccag tccaggaaaa cctaattcgc ggaccgtgcg
     7321 cacatcctca agaatggcgg caaactcacc gtcgctgtag caaaaatcac caccgcgtgg
     7381 gcgaattatc ggatgcacag ggatcgtcac ccgctggcgc acggatttca gtacccccaa
     7441 cgacggcgtt aagcccccct cttttggggc tgcgcataat tcaactctgt ctgcgccgtt
     7501 ttgctgcgcc gttagtgcac attccatgct gtaacagcaa atttccagta atgccatttt
     7561 tgctccttaa ttacgctgac tgctcgctgg caacgatctc ttcaatggac cacggatgaa
     7621 acttaatagt tgttttgcca tcggtgaccg ccagcgtcgg attcggtaat cgctcatgct
     7681 caccttttgg tgaactggtt ttaactgaga tccccggtag gctgagtcct tcatcagaaa
     7741 gcaatgccag cgcacgggca ttcagcgttt ctggatcacc cggcagaacg atttcaatat
     7801 gttcccaacc ttcgtgtggg taacgttttt ccccgggcca cggtagctcc acattggaaa
     7861 actgccagtg cgcaacctgt accggttcat gcaatttaaa cagacaaatc ggtctgccat
     7921 tgatcatttt ttctgacaaa agctcgccac actgttcaaa accgcgacgc cagcgctcag
     7981 cggtgacgtt ttgatggcaa cgcaaagaaa tgtgatcggc agtcagcgga gtgatatcca
     8041 accccagacg gcgggaaagt tcatctaatg cgtgggtaaa tcgcggtaaa tccgatgcaa
     8101 tatcctgcag ctcgtcgata gattgccagt tcgccataat cactcttcgt ctttcagtaa
     8161 aagcgttaat ttaccctgtt gccctgtgcc aaccaaccgc tgatttcacg ccgcttctga
     8221 tgcaatagtg aaaacggcaa tacgccacgc gcacgttgct gacgaaaaca gccatttgca
     8281 gtatactccc gccctaattt ctttaactgg tgcgggcaat ttttgctcgc ttcatcaatg
     8341 taaggtattc cggtgaatat tcaggctctt ctctcagaaa aagtccgtca ggccatgatt
     8401 gcggcaggcg cgcctgcgga ttgcgaaccg cagattcgtc agtcagcaaa agttcagttc
     8461 ggcgactatc aggctaacgg catgatggca gttgctaaaa aactgggtat ggcaccgcga
     8521 caattagcag agcaggtgct gactcatctg gatcttaacg gtatcgccag caaagttgag
     8581 atcgccggtc caggctttat caacattttc cttgatccgg cattcctggc tgaacatgtt
     8641 cagcaggcgc tggcgtccga tcgtctcggt gttgctacgc cagaaaaaca gaccattgtg
     8701 gttgactact ctgcgccaaa cgtggcgaaa gagatgcacg tcggtcacct gcgctctacc
     8761 attattggtg acacagcagt gcgtactctg gagttcctcg gtcacaaagt gattcgcgca
     8821 aaccacgtcg gcgactgggg cactcagttc ggtatgctga ttgcatggct ggaaaagcag
     8881 cagcaggaaa acgccggtga aatggagctg gctgaccttg aaggtttcta ccgcgatgcg
     8941 aaaaagcatt acgacgaaga tgaagagttt gccgagcgtg cacgtaacta cgtggtaaaa
     9001 ctgcaaagcg gtgacgaata tttccgcgag atgtggcgca aactggtcga catcaccatg
     9061 acgcagaacc agatcactta cgatcgtctc aacgtgacgc tgacccgtga tgacgtgatg
     9121 ggcgaaagcc tctacaaccc aatgctgcca ggaattgtgg cggatctcaa agccaaaggt
     9181 ctggcagtag aaagcgaagg ggcgaccgtc gtattccttg atgtgtttaa aaacaaggaa
     9241 ggcgaaccga tgggcgtgat cattcagaag aaagatggcg gctatctcta caccaccact
     9301 gatatcgcct gtgcgaaata tcgttatgaa acactgcatg ccgatcgcgt gctgtattac
     9361 atcgactccc gtcagcatca acacctgatg caggcatggg cgatcgtccg taaagcaggc
     9421 tatgtaccgg aatccgtacc gctggaacac cacatgttcg gcatgatgct gggtaaagac
     9481 ggcaaaccgt tcaaaacccg cgcgggtggt accgtgaaac tggcagacct gctggatgaa
     9541 gccctggagc gtgcacgtcg tctggtggca gaaaagaacc cggatatgcc agctgacgag
     9601 ctggaaaaac tggctaacgc ggttggtatt ggtgcggtga aatatgcgga tctctccaaa
     9661 aaccgcacca cggactacat cttcgactgg gacaacatgc tggcgtttga gggtaatacc
     9721 gcgccataca tgcagtatgc ttacacgcgt gtattgtccg tgttccgtaa agcagaaatt
     9781 aacgaagagc aactggctgc agctccggta atcatccgtg aagatcgtga agcgcaactg
     9841 gcagctcgcc tgctgcagtt tgaagaaacc ctcaccgtgg ttgcccgtga aggcacgccg
     9901 catgtaatgt gtgcttacct gtacgatctg gccggtctgt tctctggctt ctacgagcac
     9961 tgcccgatcc tcagcgcaga aaacgaagaa gtgcgtaaca gccgtctgaa actggcacaa
    10021 ctgacagcga agacgctgaa gctgggtctg gatacgctgg gtattgagac tgtagagcgt
    10081 atgtaatcga tttttcgtga gagtgaagcc tgatcagggt tagccgatca ggctttttta
    10141 ttgccatcta aatgtattct gaaaacggac atgccattgt tttctcactg ttgagtaaga
    10201 ggccagaagc gtaatatcca ggcccaggaa aacgataacg gttcaattta aggaataccg
    10261 aagtgtttaa atttcttgta ttaacattag gcattatctc ttgccaggct tacgcagaag
    10321 atacggttat agtaaacgac catgacactt cagccatcaa agattgttgg caaaaaaatt
    10381 cagatgatga tactgacatt aacgtgatca aatcatgcct gcgacaagaa tacaatctcg
    10441 tcgatgcgca attaaataaa gcctatggtg aagcttatcg ttatatagaa caagtgccac
    10501 gcacaggtgc aaaaaaactt gatactgaac aacttaactt gcttaaaaaa tcacagcgag
    10561 cctggctgga ttttagggac aaagaatgtg aattaatcct ttcaaatgag gatgttcagg
    10621 atttaagtga cccctattct gaatcagaat ggctatcatg tatgatcata cagaccaata
    10681 cgcgaactcg ccagttgcag ctataccgta actctgaaga tttttatcca agccctttga
    10741 caagaggata attcacatct ttttggcatg ttttgttgca agctattcct gataaatgat
    10801 tgcaacaaaa catccagcct tttgactgag ttattaaaca tactcgcgag cgcgtaattt
    10861 tttgtctttc agcgataatt cacaatcact tcattccgtt gtacctttag cggtggaatt
    10921 aaccgc
//
LOCUS       DAEBXN010000127        10813 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.127, whole genome shotgun sequence.
ACCESSION   DAEBXN010000127 DAEBXN010000000
VERSION     DAEBXN010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10813
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.127"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(172..1197)
                     /gene="tdh"
                     /locus_tag="KJL99_003546"
     CDS             complement(172..1197)
                     /gene="tdh"
                     /locus_tag="KJL99_003546"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HBD4438139.1"
                     /translation="MKALSKLKAEEGIWMTDVPVSELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(1207..2403)
                     /gene="kbl"
                     /locus_tag="KJL99_003547"
     CDS             complement(1207..2403)
                     /gene="kbl"
                     /locus_tag="KJL99_003547"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HBD4438140.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(2678..3535)
                     /gene="yibB"
                     /locus_tag="KJL99_003548"
     CDS             complement(2678..3535)
                     /gene="yibB"
                     /locus_tag="KJL99_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HBD4438141.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRIEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDENKMHLFTIKKGLTVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            3839..4771
                     /gene="rfaD"
                     /locus_tag="KJL99_003549"
     CDS             3839..4771
                     /gene="rfaD"
                     /locus_tag="KJL99_003549"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HBD4438142.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            4781..5827
                     /gene="rfaF"
                     /locus_tag="KJL99_003550"
     CDS             4781..5827
                     /gene="rfaF"
                     /locus_tag="KJL99_003550"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HBD4438143.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            5831..6823
                     /gene="rfaC"
                     /locus_tag="KJL99_003551"
     CDS             5831..6823
                     /gene="rfaC"
                     /locus_tag="KJL99_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HBD4438144.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            6820..8028
                     /gene="rfaL"
                     /locus_tag="KJL99_003552"
     CDS             6820..8028
                     /gene="rfaL"
                     /locus_tag="KJL99_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HBD4438145.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEVFKAII
                     ENTLLCTIAIPVILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(8064..9206)
                     /locus_tag="KJL99_003553"
     CDS             complement(8064..9206)
                     /locus_tag="KJL99_003553"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HBD4438146.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKLAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMYCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
     gene            complement(9215..10228)
                     /locus_tag="KJL99_003554"
     CDS             complement(9215..10228)
                     /locus_tag="KJL99_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--(galactosyl) LPS
                     alpha1,2-glucosyltransferase"
                     /protein_id="HBD4438147.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILEKNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKALPQS
                     NIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDA
                     MQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQRLTEKFFDLLSDESIIKKLKYPDQ
                     DILNLMFLHHAKILPRKYNCIYTIKSEFEEKNSEYYTRFINDDTVFIHYTGITKPWHD
                     WANYASADYFRNIYNISPWRNIPYKKAVKKHEHKEKYKHLLYQKKFLDGVFTAIKYNV
                     MKG"
     gene            complement(10253..>10813)
                     /gene="rfaY"
                     /locus_tag="KJL99_003555"
     CDS             complement(10253..>10813)
                     /gene="rfaY"
                     /locus_tag="KJL99_003555"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HBD4438148.1"
                     /translation="IDTARGPLVLKVYAPKHKMTERFLKSCIKKDYYENLIYQTDRVR
                     GEGIQSINDYFLLAERKTLNFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKEL
                     HQHGMVSGDPHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDLERHYNIKNELKDFG
                     YTYLIFKKKIKKVIRDVKVKLGLKSK"
BASE COUNT         3060 a         2429 c         2251 g         3073 t
ORIGIN      
        1 tgtgagatat tatttttaca ggtgagagag tcacttcagg gtagagagat aatgagaaca
       61 gctatataaa agatcagcgc aattacgaac tctttattac actgacagga ttttacataa
      121 ttaacagatt atcctcagat aaaagggctg gaataccagc ccttgttcgt gttaatccca
      181 gctcagaata actttcccgg actggcccga acgcatagcg tcaaagccct tctggaaatc
      241 atcgatagag aaacgatggg taatgatcgg cgagagatcg aggccagact gaatcagcgc
      301 cgccatcttg taccaggttt caaacatctc acgaccgtaa atgcctttaa tgaacaagcc
      361 tttaaagatc actttggtcc agtcgataga catatcagac ggtggaatac ccagcatcgc
      421 aatacgaccg ccgtggttca tggtatcgag catggtacga aacgctggcg gcgcgccgga
      481 catttccaga ccgacatcaa agccttcggt catgcccagt tcagccatca catcattaag
      541 gttttctttc gcgacgttaa ccgcacgggt gatacccatt ttgcgcgcca gctcaaggcg
      601 gtattcgtta acatcggtga tcaccacatt gcgtgcacca acgtgtttcg ccaccgccgc
      661 agccataata cctatcggac ctgcaccaga aaccagcaca tcttcgccca ccagatcgaa
      721 cgacagcgcg gtatgcacgg cgttaccgaa gggatcaaaa atggaagcca ggtcgtcgga
      781 gatattgtcg gggattttga aagcgttgaa cgccggaatc accagatatt cggcgaagca
      841 gcccggacgg ttaacaccga caccaatggt gttgcggcac agatgggtac gaccgccacg
      901 acagttgcga cagtgaccgc aggtgatatg gccttcgcca gaaacgcgat cgccgatttt
      961 gaagcctttc acttcctgac caatacctac cacttcaccg acatattcat ggcccacgac
     1021 catcggcacc gggatggttt tttgcgacca ctcatcccag ttatagatgt gaacgtcagt
     1081 cccgcagatg gctgttttac ggattttaat cagcagatcg ttatgcccga gttccgatac
     1141 agggacgtcg gtcatccaga tgccctcttc cgctttcagt ttggataacg ctttcatctc
     1201 acatcctcag gcgataacgc ccagttgttt accaatacgc gtgaatgctt ctactgcacg
     1261 cgtaatttgc tcaggggtat gcgccgcaga catctgggta cgaatacgcg cctgaccttt
     1321 cggaacgacc ggatagaaga aaccggtgac gtaaatgccc tctttttgca gctctcgggc
     1381 aaatttctgc gccactaccg catcaccaag catgaccgga ataatggcgt gatcggctcc
     1441 cgccagggta aagcccgccg ccgacatttg ctcacggaac tgacgcgcgt tcgcccacag
     1501 acggtcacgc aactcgctac ccgcttcaac catctccagc actttgatgg aagcggcgac
     1561 aattgctggt gccagcgagt tggagaacag gtacggacga gaacgctggc gcagccactc
     1621 aaccacttct ttgcgcgccg cggtataacc accagaagcc ccgcccagcg ctttaccgag
     1681 cgtgccggtg atgatgtcga cacgccccat cacatcgcag tattcatggg aaccacgacc
     1741 attttcaccg acaaaaccga ccgcgtggga gtcgtctacc atcaccaggg catcgtattt
     1801 atctgccaga tcgcaaacgc ccttcagatt ggcaatcacg ccgtccatcg agaacacacc
     1861 atcggtggca atcagcacat gacgcgcacc ggcttcacgc gcttctttca gacgagcttc
     1921 aagctcctgc atatcgttgt tggcatagcg atagcgttta gctttacaca gacgcacacc
     1981 atcaataata gacgcgtggt tcagtgcgtc ggagataatg gcgtcttccg cgcccagcag
     2041 cgtttcaaac aggccaccgt tagcatcaaa gcaggaagag tagagaatcg catcttccat
     2101 ccccaggaat gccgccagtt tttgttcaag ctctttatgg ctgtcctgag tgccgcaaat
     2161 aaaacgcacc gaagccatgc cgaaaccgtg agaatccatt cccgcctttg ctgccgcaat
     2221 cagatccgga tgattcgcca gcccgagata gttgttggca caaaagttaa tgacgtggct
     2281 tccatcagcc acggtgatat ctgcttgctg cgcagacgta ataatgcgct cttctttaaa
     2341 caacccttcc gcccgtgcgg tttccagatc gttggttaac tgctgataaa attctccacg
     2401 cattgcgatt ctccagacag ggcaaattcc agcacatatt acccaaactt ataggtcgcg
     2461 acgagatgat gcgttaacac ttctgcaaaa ttcaggataa ataacgtgta tcttgatgtc
     2521 tctcaacacc tacagatagt agaaaaactg tacagttttg gctgagcata atgatgagtg
     2581 attttactgc attgatataa aaagaaaaaa tgatgccaaa gcatcatttt tcctatctca
     2641 gttggcatca aaacgctacc cactaatgga gggaaaccta ttttcttgaa aggaaaattc
     2701 tcagcgcctg catttttgcc cctaaagtgt tactcctgaa gcagcgaaac aaatcgaacc
     2761 attttcctct ccccagatag tttaggttga agagatcgga acgtttgtaa tagcacatga
     2821 caaaaatgcc ctggtcatcg tccacaatgt tattgtttag tgttattttt tgactttcca
     2881 gaactaattt atagaattct ttccatttat gctgtgaacc aacaattgcg cccccaataa
     2941 tatatacatg attgccaatc atgaaatcaa aagcctgttg ttgagatgta acagtaagcc
     3001 ctttttttat agtgaaaaga tgcatcttat tttcatcaaa tgggaaatcc caaattttca
     3061 atcctcgagt tacatttggc ttacggcaat agccaaaatc tatccacgca acaagaggcg
     3121 ttttcactaa acccatgttg atagctttat ttacaaaata ggccttaagg ttacatacca
     3181 aaacatactc tggtgaccag tactctgggt tttttaattg tcgaggctca agtctatttg
     3241 taaatgactc atctttttga attttttcga tacgacttct gatatatcta aatttctttt
     3301 ttatatcgat aacgataacc gttgttggtt ttccgttgcg aatcgcttca atccttggtt
     3361 tcaaatcggg agaagtgaaa ataatcatct cattttcaag tgcggccaga cgctcaaagt
     3421 aactaaaata aacatcaact gaacgagcta gtttttcacg gaacccttta ttagcagtcc
     3481 aatcccctct accaatgtca aaatatgcag tgataattgt ggtggatgat ttcattacta
     3541 ataagaccat taatataaaa aaagaattat atcataaata ttaacagaat gtatactgta
     3601 tattatccat tcaaatgatt ccgttattta tcatacatcg gaatattgat actaaagcac
     3661 tattcacatg caaaaccaac atccgccatg aaggactagc taaaacccaa actagtttgt
     3721 tgcaattagc atccttgcac ctctatgtaa agggctgaag ggattcggat gtgatggtat
     3781 gattacagac attcgtgtct gagattgtct ctgactccat aattcgaagg ttacagttat
     3841 gatcatcgtt accggcggcg cgggctttat cggcagcaac atcgttaagg ccctgaatga
     3901 taaaggcatc accgatattc tggtggtgga caacctgaaa gacggcacca agtttgtgaa
     3961 cctggtggat ctggatatcg cggactatat ggataaggaa gacttcctga tccagattat
     4021 ggctggcgaa gagttcggtg atgtcgaagc gattttccac gaaggcgcat gctcttccac
     4081 caccgagtgg gatggcaagt atatgatgga taacaactat caatactcca aagagctgct
     4141 gcactactgt ctggagcgcg aaatcccgtt cctgtatgcc tcttccgcag ccacctacgg
     4201 cggacgcacc tccgacttta ttgaatcccg cgagtacgaa aaaccgttga atgtctacgg
     4261 ttactcaaaa ttcctgtttg atgaatatgt tcgtcaaatc ctgccagaag cgaactcgca
     4321 gattgttggc ttccgctatt tcaacgttta tggaccgcgt gaaggccata aaggcagcat
     4381 ggcgagcgtc gctttccatc tcaacaccca gcttaataac ggtgaatcac cgaagctgtt
     4441 cgaaggtagc gagaacttca aacgtgactt cgtctatgtg ggcgacgtgg cagatgtaaa
     4501 cctgtggttc ctggaaaatg gcgtttccgg tatcttcaac ctcggcaccg gtcgtgcaga
     4561 atccttccag gcagtagcag atgctacgct ggcttatcac aagaaaggcc agatcgaata
     4621 cattccgttc ccggataagc tgaaaggccg ctaccaggcg ttcactcagg cagatctgac
     4681 aaatctgcgc gcggcgggtt acgacaaacc gttcaaaacc gttgctgaag gtgtaacgga
     4741 atacatggcc tggctgaatc gcgacgcata agagctctgc atgaaaatac tggtgatcgg
     4801 cccgtcttgg gttggcgaca tgatgatgtc gcaaagtctc tatcgcacgc tccaggcgcg
     4861 ctatccccag gcgataatcg atgtgatggc accggcatgg tgccgtccat tattatcgcg
     4921 gatgccggaa gttaacgaag ctatccctat gcctctcggt cacggagcgc tggaaatcgg
     4981 cgaacgccgc aaactgggtc atagcctgcg tgaaaagcgc tacgaccgcg cctacgtctt
     5041 accaaactcc ttcaaatctg cattagtgcc tttcttcgcg ggtattcctc atcgcaccgg
     5101 ctggcgcggc gagatgcgct acggtttact caacgatgta cgcgtgctcg ataaagaagc
     5161 ctggccgcta atggtggaac gctatgtcgc gctggcctat gacaaaggca ttatgcgtac
     5221 cgcacaagat ctgccgcagc cattgttatg gccgcagttg caggtgagcg aaggtgaaaa
     5281 atcatatacc tgtaatcaat tttcgctttc atcagaacgt ccgatgattg gcttttgccc
     5341 gggtgcggag tttggtccgg caaaacgctg gccacactac cactatgcgg aactggcaaa
     5401 gcagctgatt gatgaaggtt atcaggtggt tctgtttggc tctgcgaaag atcatgaagc
     5461 gggcaatgag attcttgccg ctttgaatac cgagcagcag gcatggtgtc ggaacctggc
     5521 gggggaaaca cagcttgatc aagcggttat cttgattgca gcctgtaaag ccattgtcac
     5581 taacgattct ggcctgatgc acgttgcggc ggcgctcaat cgtccgctgg ttgccctgta
     5641 tggtccaagt agcccggact tcacaccgcc gctatcccat aaagcgcgcg tgatccgtct
     5701 gattaccggc tatcacaaag tgcgtaaagg tgacgctgcg gagggttatc accagagctt
     5761 gatcgacatt actccccagc gcgtactgga agaactcaac gcgctattgt tacaagagga
     5821 agcctgacgg atgcgggttt tgatcgttaa aacatcgtcg atgggcgatg ttctccatac
     5881 gttgcccgca ctcactgatg cccagcaggc aatcccaggg attaagtttg actgggtggt
     5941 ggaagaaggg ttcgcacaga ttccttcctg gcacgctgcc gttgagcgag ttattcctgt
     6001 ggcaatacgt cgctggcgta aagcctggtt ctcggccccc ataaaagctg aacgcaaagc
     6061 gtttcgtgaa gcgctacaag cagagaacta tgacgcagtt atcgacgctc aggggctggt
     6121 aaaaagcgcg gcactggtga cacgtctggc gcatggcgta aagcatggat tggactggca
     6181 aaccgctcgc gaacctttag ccagcctgtt ttacaattgt aagcatcata ttgcaaaaca
     6241 gcagcacgcc gtagaacgca cccgcgaact gtttgccaaa agtttgggct atagcaaacc
     6301 gcaaacccag ggcgattatg ctatcgcaca gcattttctg acgaacctgc ctacagatgc
     6361 tggcgaatat gccgtatttc ttcatgcgac gacccgtgat gataaacact ggccggaaga
     6421 acactggcga gaattgattg gtttactggc tgattcagga atacggatta aacttccgtg
     6481 gggcgcgccg catgaggaag aacgggcgaa acgactggcg gaaggatttg cttatgttga
     6541 agtattgccg aagatgagtc tggaaggcgt tgcccgcgtg ctggccgggg ctaaatttgt
     6601 agtgtcggtg gatacggggt taagccattt aacggcggca ctggatagac ccaatatcac
     6661 ggtttatgga ccaaccgatc cgggattaat tggtgggtat gggaagaatc aagtagagtg
     6721 tcgttctaca agtatgtctc ttgcagattt gccagctcaa accgtttttc aaaatttaaa
     6781 tcttgaaata ataaccaata agttgacatc ggagataaga tgacctcaac attatttttc
     6841 tctctcgaga aaaaaaactg gatagcgtac tggaacagag ctctcgtatt cttattcatt
     6901 accacctatt ttttgggtgg gattacaagg tataaacatc ttattgttat tcttatgaca
     6961 ataacgacaa tcgtctatct ctgcaaacgg ccaaaacact atctctcact atttaaaaca
     7021 tttctttttg gtagtgttgc catattaact attgctgcat tgctgtcact tcttcaatcc
     7081 cctgatgcag gtgctagcat gaaggaagtt ttcaaagcta ttattgagaa tactttacta
     7141 tgcacaatag caataccggt catattgaga gacgagaaaa gagaagatgt cgaaaaaatc
     7201 gttttcttct catttattag tgcgttgggc ttacgctgtt tttctgaatt gattacctat
     7261 tataaggact atcaacaagg gataatgcca ttcgcagatt atagacaccg tagcatttct
     7321 gactcgatgg tctttttatt ccctgcattg ttaaatctct ggcttatcaa atcagcaaaa
     7381 taccgcattt cttttgtggt tctaagcgtt atttttattt ttctgatatt aggaacttta
     7441 tccagagggg cctggctttc cgtgttagtc attggattaa tatggattct gatgtttaaa
     7501 caatggaagt tactattagt aggagtaatg gtttccatca ttgcattgtc ggttattttc
     7561 acacataagg agatgaccgc aaagctaacg tataaacttc aacaaactaa tagttcttat
     7621 cgctatgcaa atggtactca aggcagcgca ctcgatctaa tattagaaaa tcctgttatt
     7681 ggttatggtt acggtaacgt tgcatataaa gatgtctata ataaacgtgt cattgattat
     7741 ccagaatgga cctttagaca atcaataggg ccacataatt ttgcgctatt catctggttt
     7801 ggcactggtt tattagggct ggtaagtctt atgatgctat actgtgcaat attgaaagag
     7861 tgtataaaaa atggcgtcaa gaataaatat cgctcaccat ataatgcata ttatataatc
     7921 ttactatctt ttataggtta ttttgttatc cgtggaaacg tagaacaaat tgaaccaaat
     7981 ttattaggcg tttacgccgg cttattatta gcgatgaaaa acaagtaaaa aataaaaaag
     8041 gctgcataat gcggcctttt tatttattca aaccaattat gaataacctc ttcgaatctt
     8101 tgagttacgc catcccaact gtagtgatca aacacaaagt cctgaccttg ctttgcaact
     8161 gctgttaatt caggattcgc taacgttttt aaaatatcgc tgcttatgga gtctgcagtc
     8221 ataggttctt ttaaatggaa tcccgtagta ttttctttga caaattccgt catcccccca
     8281 cgcgtactca ctaaaacagg cttaccagca cccatagctt caattgccac catgcaaaat
     8341 ggttcctgga actgggaagg aatgacgact aaatctgcca gtgggtaata acaatacatc
     8401 ttttcaggcg gtatgcttcc taacataatg caacgatcct tcaaacgttt tgcaatttcc
     8461 ctgacattgc gttgatatgc acctttgtca cttttactca tttctgtata atcaccaatc
     8521 acaactaatt ttaaattttt gtgagcagcg gccaattttt cgaatgattg catcaacagc
     8581 aatatgcctt tgtcagggac gatacgccca gcataaaaaa taattttctc ttcaggagag
     8641 atattaagtt cgcttctagt gatcggctga aaattagatt gataggtttc taaatcaatt
     8701 ccatttggta caatctcaat atctgcattt gctaaataag attggtaata ctttttcaag
     8761 tataaactag ggactatcat tttaacattt tgctcaagca attttggttc aaatgcatta
     8821 tgcatatgta taaccactct tgcctgagga gctagcttac gaatttgagt gtatagcttc
     8881 atgctattat gaactacaat aacactttct ttcgttatag gaaaatcatg tgcaatattc
     8941 aatattctct gtgaatatgg aagaggatca agtcttgtcc atttttgaaa taaccgctta
     9001 tatatgcgac tgaatccaac cctatgaata ctacaattat cattaacaaa ggtgtaattt
     9061 gagtatcctg gatttttgat acaaacaata cgatttggaa aattggtacg ctgagcaacc
     9121 tgatacatcc acgtttcaac agcagccgct ccgcgaggag gaattgaaaa aataggagta
     9181 accgtaaata ttattttatc aaccatctcg tatattaacc tttcataaca ttatatttaa
     9241 ttgctgtaaa aacaccatcg agaaattttt tctggtaaag caagtgttta tatttttctt
     9301 tgtgctcatg ttttttaaca gcttttttat aaggtatatt tctccatggt gatatattat
     9361 aaatattacg aaaataatct gcagaggcgt agttcgccca atcatgccat ggcttagtta
     9421 taccagtata atgtatgaag acagtgtcat cattaataaa ccgggtgtaa tattcactat
     9481 ttttttcttc aaattctgac tttatagtat aaatacaatt atattttctc ggtaatattt
     9541 tagcatgatg tagaaacatt aagtttaaaa tatcttgatc cgggtacttt aattttttta
     9601 taattgactc atctgataat agatcaaaga atttttccgt tagtctttgt tttaaccatt
     9661 cccgtaaatt aatatacatt acaccagagt taaaatagct accgttaaaa tcctcattac
     9721 acaaacgctc agcgctttta ctttgcatag catctacatc aattaccact gccccatatt
     9781 catctttaaa ctctaatgct attaactcgt tcaatgaacc cttacagacg atatcagcat
     9841 caagatataa taatgaatcc aatcgcgcag aaaaataatc aaatgaaagc aaacgataat
     9901 aaatagatac tggccagata tttgattgag gtagcgcctt caatggctca gaatcaatat
     9961 gatataattt aattactgtt tgatacttcg ttgctaattg tgataataat tcaatatact
    10021 ctttgtctaa gtaatcagcg attatgtgaa aggtaaattt gttttgttta ttcttttcaa
    10081 gaattgaagc aattgaaatc gcagccccaa cctggtagtt ctcatcaata ccccatgaga
    10141 cattaaacgt ttcccgttca tcaagtttaa cggggcgctt gtccagttca attatatctt
    10201 taaattgagt aagatgttta aaatccaatt taattaccct tcatcataat cattatttgc
    10261 tcttcaatcc tagtttgact tttacatccc tgattacttt tttaattttc tttttgaata
    10321 taagatatgt atagccaaag tctttcaatt cattttttat attatagtgc cgttcaagat
    10381 caatgcgatc ttttgctttt aaaacagcgg ttgttttttt acctgaaagg tcaattagtc
    10441 ttaaaccttt ttcggatact ataaaatttc ctttatgagg atcgccagac accattccat
    10501 gctgatgcaa ctcttttatc gattctgata attgatcctt cagatcttca gaaatttcca
    10561 aatactcatt taacccgaca ccttcaatat actcaatgag cataatataa taatgagcga
    10621 aatttaatgt tttacgttca gctagcaaaa aatagtcatt aattgattgg atgccttcac
    10681 cacgtacccg atcggtttga taaattaaat tttcataata atcttttttt atgcaagatt
    10741 ttaagaaacg ttcggtcatt ttatgctttg gcgcataaac tttaagtaca agaggtccac
    10801 gagcagtgtc aat
//
LOCUS       DAEBXN010000128        10672 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.128, whole genome shotgun sequence.
ACCESSION   DAEBXN010000128 DAEBXN010000000
VERSION     DAEBXN010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10672
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.128"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            2..2254
                     /locus_tag="KJL99_003556"
     CDS             2..2254
                     /locus_tag="KJL99_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438149.1"
                     /translation="MFSIKPGPGNLPIDNPTLLSWNITDGDLNSKFNTLEYLNCITNI
                     INACGVYPQDLKDREIISTFHAEKVINDLLKNDYKISLSPDTTYRELNKAAQSSITAP
                     DRIGEGKTWVYQRDTMVERGDNSGVHQYGPAEHFTHIISDKPSPKDKYVAYAINIPDY
                     ELAADVYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEIITPKKPGEA
                     ILHAFNATYQQIRENMSEFARSHYGYIQIPPVTTFRADGPETPEEEKGYWFHAYQPED
                     LCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDYPSADIRAKI
                     SKDIESGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKDNISKDEWKN
                     LHALKEKSLNGMPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHSKHEQIERSI
                     TPPLKNYNERLHNTNLFNPKYHEKTVMNYMEYLVTCQLNEEQMSSLLYERDDLSAIDF
                     SLLMKTIKAFSFGGDLLTLASKPGSTISSIPSERRILININHDFPNNGNLFNDFLFNH
                     QQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGPTRVGGEAWLNKGCSTYQQ
                     LYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVIINKGNEEVE
                     GTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPCKVFTLRGGSLNINIPRRSALLLGK
                     TGEPPNYLYL"
     gene            2590..3522
                     /gene="csiD"
                     /locus_tag="KJL99_003557"
     CDS             2590..3522
                     /gene="csiD"
                     /locus_tag="KJL99_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HBD4438150.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDDWEHLDHYFRHPLARRPMRFAAPPSKNVSKDVFHPVFDV
                     DQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVPVPVGKFLLINNLFWL
                     HGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            3542..4810
                     /gene="lhgO"
                     /locus_tag="KJL99_003558"
     CDS             3542..4810
                     /gene="lhgO"
                     /locus_tag="KJL99_003558"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HBD4438151.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            4833..6281
                     /gene="gabD"
                     /locus_tag="KJL99_003559"
                     /pseudo
     CDS             4833..6281
                     /gene="gabD"
                     /locus_tag="KJL99_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
     gene            6289..7575
                     /gene="gabT"
                     /locus_tag="KJL99_003560"
                     /pseudo
     CDS             6289..7575
                     /gene="gabT"
                     /locus_tag="KJL99_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307963.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
     gene            7813..9213
                     /gene="gabP"
                     /locus_tag="KJL99_003561"
     CDS             7813..9213
                     /gene="gabP"
                     /locus_tag="KJL99_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HBD4438152.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAD
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDMPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLDPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            9234..9896
                     /gene="csiR"
                     /locus_tag="KJL99_003562"
     CDS             9234..9896
                     /gene="csiR"
                     /locus_tag="KJL99_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HBD4438153.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLQ
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLPRDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(9897..10346)
                     /gene="kbp"
                     /locus_tag="KJL99_003563"
     CDS             complement(9897..10346)
                     /gene="kbp"
                     /locus_tag="KJL99_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HBD4438154.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(10430..10588)
                     /locus_tag="KJL99_003564"
     CDS             complement(10430..10588)
                     /locus_tag="KJL99_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HBD4438155.1"
                     /translation="MGFWRIVITIILPPIGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
BASE COUNT         2698 a         2646 c         2746 g         2582 t
ORIGIN      
        1 tatgttttct ataaaaccag gacctggaaa tttacctatc gacaacccca cattgttatc
       61 atggaacatt actgatgggg atctaaattc caaatttaat acattagaat atctaaactg
      121 tataacaaat attattaatg cttgtggagt ttatcctcag gatttaaaag acagagaaat
      181 tatatcaact tttcacgcag aaaaagtcat taatgatctg ttaaaaaacg attataaaat
      241 ttccctttct ccagatacaa cttaccgaga gctgaataaa gctgcacaga gtagcattac
      301 agcgccagac aggataggag aagggaaaac atgggtttat caacgagata caatggttga
      361 aagaggtgat aacagtggtg ttcatcagta tggtccagct gaacatttca ctcacattat
      421 atctgacaaa ccgtccccaa aagataagta tgttgcatat gctattaaca ttcctgacta
      481 tgagctagca gccgatgtat ataatattaa cgtgacgtca ccttccggac agcaagaaac
      541 atttaaaata ctaatcaatc cagaacatct acggcaaaca cttgagcgta aatctcttac
      601 tgctgttcag aaatcacaat gtgaaatcat cacccccaaa aaacctggcg aagcgattct
      661 tcatgctttt aatgccacct accagcaaat cagggaaaat atgtctgagt ttgcacgttc
      721 ccattatggg tatatacaaa tccctccagt gacaactttc cgcgccgacg gaccagaaac
      781 tcccgaagaa gaaaaaggtt actggtttca cgcttatcaa cccgaagatc tttgtaccat
      841 ccataatcca atgggggatt tgcaggattt tatcgcattg gttaaagatg ctaaaaaatt
      901 tggtatcgat atcattcctg attatacctt taactttatg ggaatcgggg gaagtggtaa
      961 aaatgacctg gattatccct ctgctgatat acgagcgaag atcagtaaag atatagaaag
     1021 tggtatccct ggctattggc aaggtcaggt tttgattcca ttcattaaag atccagtaac
     1081 aaaagaacgt aaacaaatcc atccagaaga tatacatctc actgcaaaag acttcgaagc
     1141 aagtaaagat aacatctcta aggatgaatg gaaaaacctc catgcattaa aagaaaagag
     1201 tttaaatgga atgcctaaaa caacccccaa aagtgaccag gttattatgt tgcaaaatca
     1261 atacgttcgt gaaatgcgaa aatatggcgt acgaggttta cgttatgatg cagcaaaaca
     1321 ctcaaaacat gaacaaatag aaagatcaat aaccccaccg cttaaaaatt ataatgagcg
     1381 attacacaat actaacttat ttaacccaaa atatcatgaa aaaaccgtta tgaattacat
     1441 ggaatatctg gtaacttgtc agttgaatga agaacaaatg tcatcgctgc tttatgaaag
     1501 agatgattta agcgccattg atttttcatt gctcatgaag acgataaaag ccttttcatt
     1561 cgggggagat ctcctaaccc ttgcatcaaa accgggttcc acaatctcaa gcatcccatc
     1621 agaaagacgg atattgatta acattaacca cgattttcct aacaatggca atcttttcaa
     1681 tgactttcta tttaaccatc aacaagatga acaattagca atggcatata tggccgctct
     1741 cccgttcagc aggcctttag tttactggga tggccaggta ttaaaatcaa cgactgaaat
     1801 taaaaattat gatgggccca cgcgtgtcgg tggtgaggcg tggcttaata aaggttgctc
     1861 tacctatcag cagctctaca atgaattcca cgcattatat atagataaag caggaatatg
     1921 gagcgcattt gagggtgtat ttgcaactaa aaacgttctg gcctttagtc gtggggattc
     1981 tgtgaacatt aatcactctc ctcatgatgg actagttata ataaataaag gaaacgaaga
     2041 agttgaaggt acctggccta acaaattgca acctggaata tataaaaaca tggggagtaa
     2101 tagcgttaac attattatta ataatacccg aaaaattatc cccccttgta aagtatttac
     2161 gcttagaggc ggatctctaa atatcaatat tcctaggcgt agcgctcttc ttttagggaa
     2221 aacaggagaa ccgccgaact atctctattt ataatttaat gttatatctg ccccgataaa
     2281 acggggcaga taatatgttt agtttactaa cggtcatttt gtagtgaagc catttactgt
     2341 tttttatcga ccagataatc tgttctctaa tgttaactcc ccctaacctg ttgctttagt
     2401 tattcatttc ctgtctcact ttgccttaat accctacgtt aaatgttact aatttgttgc
     2461 ttttgatcac aataagaaag caatatgtcg cttttgtgcg catttttcag aaatgtagat
     2521 atttttagat tatggctacg aaatgagcat cgccatgtca ccctacatct cataagagga
     2581 tcgcttctga tgaatgcact gaccgccgta caaaataacg ctgtcgattc aggccaggac
     2641 tatagcggat tcactctcat cccgtcggcg caatccccgc gtctgctgga actcaccttc
     2701 accgaacaga cgaccaaaca gtttctcgaa caggttgccg aatggcccgt gcaggcgctg
     2761 gagtacaaat cgtttctgcg ttttcgggta ggcaaaattc ttgacgatct gtgtgcgaat
     2821 cagctgcaac cattgctgtt gaagaccctg ctaaaccgtg ctgaaggtgc gctgttgatc
     2881 aatgcggtag gtatcgatga tgtggcgcag gcagatgaga tggtgaagct ggcaacggcg
     2941 gtagcgcatc tgattggtcg ctcaaatttc gatgcgatga gcggtcagta ttacgcgcgt
     3001 ttcgtggtga aaaatgtcga taactcagac agctatctgc gtcagccgca ccgcgtgatg
     3061 gaactgcaca acgacggcac gtatgtcgaa gagatcaccg attacgtgct gatgatgaaa
     3121 atcgatgact gggaacatct ggaccactat ttccgccatc cactggcgcg tcgcccgatg
     3181 cgctttgccg cgccgccgag caaaaacgtc agcaaagatg ttttccatcc agtgttcgac
     3241 gttgatcaac agggtcgccc ggtgatgcgc tatatcgacc agttcgtcca gccaaaagat
     3301 tttgaagaag gcgtgtggtt gagcgagctt tccgatgcca ttgaaaccag caaaggcatt
     3361 ctttctgtac ccgttcccgt tggcaaattc ctgttgataa acaacctgtt ctggctgcac
     3421 ggtcgcgacc gctttactcc gcacccggat ctgcgccgtg aactgatgcg tcagcgtggc
     3481 tatttcgctt acgccactca ccactaccag acgcatcagt aagcgcaaag gaattgagcg
     3541 gatgtatgat tttgtgatta ttggcggcgg catcatcggc atgtcgaccg ccatgcaact
     3601 gattgatgtt tatccggatg cccgcattgc gttgctggaa aaagagtccg gcccggcctg
     3661 tcaccagacg ggccacaaca gcggcgtgat ccatgccggg gtctattaca cgcccggcag
     3721 cctgaaggcg cagttttgcc tggcggggaa ccgtgccacc aaagcctttt gcgatcaaaa
     3781 cggcattcgc tatgacaact gcggcaagat gctggtcgcc acgtctcagc tcgaaatgga
     3841 acggatgcgc gccttatggg aacgcacagc ggcgaacggt atcgagcgcg agtggataaa
     3901 cgccgtggaa ctgcgcgagc gcgaaccgaa tatcaccggg cttggtggca tttttgtgcc
     3961 gtccagcggc attgtcagct accgcgaagt gacggcggca atggcaaaaa tcttccaggc
     4021 cagaggcggc gagattattt ataacgccga agtcagcgcc ctcagtgagc ataaaaacgg
     4081 cgtggtgata cgcacccgtc agggcggtga atatgaagca tcgacgctga ttagctgttc
     4141 cgggctgatg gctgaccggc tggtgaaaat gctcggcctc gaaccgggct ttattatctg
     4201 cccgttccgt ggcgagtatt tccgccttgc gccggagcat aaccagattg ttaaccacct
     4261 gatttacccc attcccgacc ccgcgatgcc atttttgggc gtgcatctca cccgcatgat
     4321 cgacggcagc gtgaccgtcg ggccaaacgc ggtgctggct ttcaaacgcg aaggctatcg
     4381 caagcgcgac ttctcattta gtgacacgct ggagattttg agctcgtcgg ggattcgccg
     4441 ggtgctgcaa aaccatctac gctcaggact gggcgagatg aaaaactcgc tgtgcaaaag
     4501 cggctatctg cggctggtgc aaaagtattg tccccggctt tcgttaagcg atctccagcc
     4561 ctggcccgcc ggtgtgcggg cgcaggcagt atcgccggac ggcaagctga ttgacgattt
     4621 tctgtttgtc accaccccac gcacgatcca cacctgcaat gcgccctccc cggcagcgac
     4681 atcagcaatt cctattggtg cgcatattgt cagcaaggta caaacgctgt tggcaagcca
     4741 gagtaacccc ggacgcacgc tgcgagcggc acgtagtgtg gatgccttac acgccgcatt
     4801 taatcaataa cctttgaaaa caggacgtaa caatgaaact taacgacagt aacttattcc
     4861 gccagcaggc gttgattaac ggggaatggc tggacgccaa caatggtgaa gtcatcgacg
     4921 tcaccaatcc ggcgaacggc gacaagctgg gtagcgtgcc gaaaatgggc gcagatgaaa
     4981 cccgcgccgc tatcgacgcc gccaaccgcg ccctgcccgc ctggcgtgcg ctcaccgcca
     5041 aagaacgcgc caccattctg cgcaactggt tcaatttgat gatgcagcat caggacgatt
     5101 tagcgcgcct gatgaccctc gaacagggta aaccgctggc tgaagcgaaa ggcgaaatca
     5161 gctacgccgc ctccttcatt gagtggtttg ctgaagaagg caaacgtatt tatggcgaca
     5221 ccattcctgg tcatcaggcc gataagcgcc tgattgttat caagcagccg attggcgtca
     5281 ccgccgccat cacgccgtgg aactttccgg cggcgatgat tacccgtaaa gccggtccgg
     5341 cgctggcggc aggctgcacc atggtgctga agcccgccag tcagacgccg ttctctgcgc
     5401 tggcgctggc ggaactggcg attcgcgcgg gcattccggc tggcgtgttt aacgtggtca
     5461 ccggttcggc aggcgcggtc ggtaatgaac tgaccagcaa cccgctggta cgcaaactgt
     5521 cgtttaccgg ttagaccgag attggccgcc agttaatgga acagtgcgcg aaagacatca
     5581 aaaaagtgtc gctggagctg ggcggcaacg cgccgtttat cgtctttgac gatgccgatc
     5641 tcgataaagc cgtggaaggc gcgctggcct cgaaattccg caacgccggg caaacctgcg
     5701 tctgcgccaa ccgtttatac gtgcaggacg gcgtgtatga ccgctttgcc gaaaaattac
     5761 agcaggcggg gagcaaactg cacatcggcg acgggctgga gaaaggcgtc accatcgggc
     5821 cgctgatcga tgaaaaagcg gtagcaaaag tggaagagca tattgccgat gcgctggaga
     5881 aaggtgcgcg tgtggtttgc ggcggtaaag cgcacgaacg cggcggcaac ttcttccagc
     5941 caaccattct ggtggacgtt ccggccaacg ccaaagtgtc gaaagaagag acgttcggcc
     6001 ccctcgcccc actgttccgt tttaaagatg aagccgatgt gattgcacaa gccaatgaca
     6061 ccgagtttgg tcttgccgcc tatttctacg cccgtgattt aagccgcgtc ttccgcgtcg
     6121 gcgaggcgct ggagtacggc atcgtcggca tcaataccgg cattatttcc aatgaagtgg
     6181 ccccattcgg cggcatcaaa gcctcgggtc tgggtcgtga aggttcgaag tatggcatcg
     6241 aagattactt agaaatcaaa tatatgtgca tcggtcttta actggagaat gcgaaggaac
     6301 agcaataaag agttaatgca gcgccgcagt caggcgattc cccgtggcgt tgggcaaatt
     6361 cacccgattt tcgctgaccg cgcggaaaac tgccgggtgt gggacgttta aggccgtgag
     6421 tatcttgatt tcgcgggcgg gattgcggtg ctcaataccg ggcacctgca tccgaaggtg
     6481 gtggccgcgg tggaagcgca gttgaaaaaa ctgtcgcaca cctgcttcca ggtgctggct
     6541 tacgagccgt atctggagct gtgcgagatt atgaatcaga aggtgccggg cgatttcgcc
     6601 aagaaaacgc tgctggttac gaccggttcc gaagcggtgg aaaacgcggt aaaaatcgcc
     6661 cgcgccgcca ccaaacgtag cggcaccatc gcttttagcg gcgagtatca cgggcgcacg
     6721 cattacacgc tggcgctgac cggcaaggtg aatccgtact ctgcgggcat ggggctgatg
     6781 ccgggtcatg tttatcgcgc gctttatcct tgcccgctgc acggcataag cgaggatgac
     6841 gctatcgcca gcatccaccg gatcttcaaa aatgatgccg cgccggaaga tatcgccgcc
     6901 atcatgattg agccggttca gggcgaaggc ggtttctacg ccgcgtcgcc agcctttatg
     6961 cagcgtttac gcgctctgtg tgacgagcac gggatcatgc tgattgccga tgaagtgcag
     7021 agcggcgcgg ggcgtaccgg cacgctgttt gcgatggagc agatgggcgt ggcgccggat
     7081 cttaccacct ttgcgaaatc gatcgcaggc ggtttcccgc tggcgggcgt caccgggcgc
     7141 gcggaagtga tggacgctgt tgcaccgggc ggtctgggcg gcacctatgc cggtaacccg
     7201 attgcctgcg tggctgcgct ggaagtgttg aaggtgttcg agcaggaaaa tctgctgcaa
     7261 aaagccaacg atctggggca gaagctaaaa gacggattgt tggcgatagc cgaaaaacac
     7321 ccggagatcg gcgacgtacg cgggctggga tcgatgattg ccatcgagct gtttgaagat
     7381 ggcgatcaca gcaaaccgga cgcaaaactc accgccgaga tcgtggcacg cgcccgcgat
     7441 aagggcctga ttcttctctc ctgcggcccg tattacaacg tgctgcgcat ccttgtacca
     7501 ctcaccattg aagacgctca gatccgtcag ggtctggaga ttatcggtca gtgttttgat
     7561 gaggcgaagc agtagcaccg tccccatgca ggaggcgacg ctgcgcgtct tgtccggcct
     7621 acgagaatcc aagtcggata aggcgtttac gccgcatccg acaatctgta cgtgaacagg
     7681 aataaatcta tgtcggccgg ataaggcgga gccgctctcc ggcaaaaaga atcaataaca
     7741 attaaacgcg tgacccgacg cgggaaatgt cggggcgctc tcccaagtga cacactttcg
     7801 agaggattca ggatggggca atcatcgcaa ccacacgagt taggcggcgg gctgaaatca
     7861 cgccacgtca ccatgttgtc tattgccggt gttatcggcg caagtctgtt tgtcggttcc
     7921 agcgtcgcca tcgccgaagc ggacccggcg gtattactgg cctatctgtt cgccggatta
     7981 ctggtggtta tgattatgcg gatgctggcg gaaatggcag ttgccacgcc cgataccggt
     8041 tcgttttcca cctatgccga taaagccatt ggccgctggg cgggctatac catcggctgg
     8101 ttgtactggt ggttttgggt actggttatc ccactggaag ccaacatcgc cgccatgatc
     8161 ctgcactcgt gggttccagg cattcccatc tggttatttt ccctcgtcat taccctcgcc
     8221 ttaactggca gtaatttatt aagcgttaaa aactacggcg aatttgagtt ctggctggcg
     8281 ctgtgcaaag tcatcgctat cctggccttt attttccttg gtgcagtcgc aattagcggt
     8341 ttttaccctt atgccgaagt gagcgggatc tcaagattgt gggatagcgg cggctttatg
     8401 cccaacggtt tcggtgcggt attaagcgcg atgttgatca ccatgttctc gtttatgggc
     8461 gcagaaattg tcaccattgc cgccgcggaa tccgacatgc cggaaaaaca tattgtccgc
     8521 gccaccaact cggttatctg gcgtatttct atcttctatt tgtgctctat ttttgtcgtc
     8581 gtggcattaa taccgtggaa tatgccgggg ctgaaagccg ttggatctta tcgctcggtt
     8641 ctggaattgc tcaatattcc ccatgcgaaa ttaatcatgg actgtgtgat attactttcc
     8701 gtaaccagtt gcctgaactc ggccctgtat accgcctcaa ggatgctcta ctccttaagc
     8761 cgtcgcggtg atgctcccgc ggtaatgggt aaaatcaacc gcagtaaaac cccgtacgtg
     8821 gcggtgttac tctccaccgg cgcagcattc ttaacggtgg tggtgaacta ttacgcgcct
     8881 gcgaaggtat ttaaatttct gatcgacagc tccggcgcta tcgccctgct ggtttattta
     8941 gtcatcgccg tttcacagtt gcggatgcgc aaaattctgc gagcagaagg aagcgaaatt
     9001 cgcttgcgaa tgtggcttga tccctggctc acctggctgg tcatcggctt tattaccttt
     9061 gtgttggtag tgatgctatt ccgtccggcg caacagttag aagtgatctc caccggctta
     9121 ttagcgatag ggattatctg taccgtgcca attatggctc gctggaaaaa gctggtattg
     9181 tggcaaaaaa cacccgttca taatacgcgc tgataatgat caggagtcac accatgacca
     9241 ttacgtctct ggatggctat cgctggctga agaacgatat tattcgcggt aattttcaac
     9301 cggatgaaaa attacggatg agtttactga catcgcgtta tgcacttggc gttgggccgt
     9361 tgcaggaagc actttctcaa ctggtggcgg aacggctggt cacggtggtg aatcaaaaag
     9421 ggtatcgggt ggcgtctatg tcagagcagg agctgctcga tattttcgac gcccgcgcca
     9481 atatggaagc gatgctggtc agtctggcga ttgcccgcgg tggcgatgaa tgggaggcag
     9541 acgttctcgc aaaagcgcat ctgctcagta agctggaagc ctgtgacgcc agcgagaaaa
     9601 tgcttgatga gtgggatctg cgtcatcagg cgtttcatac agcaattgtg gcgggctgtg
     9661 gttctcacta tttgctgcaa atgcgtgaac ggttgtttga tctggcggcg cgttatcgat
     9721 ttatctggct gcggcgaacg gtgctttcgg tggaaatgct ggaggataaa cacgatcagc
     9781 accagaccct gactgcggcg gtactgccgc gagatacagc gcgcgccagt gagttaatgc
     9841 gccagcattt actgacgcca attcccatta tccagcaggc gatggctggc aattaactac
     9901 tcttccggaa tacgcaacac ttgccccgga taaattttat ccgggctttt tagcatcggt
     9961 ttattcgctt cgaagatttt attgtacaga ttagcgttac cgtagacctg tttggaaatg
    10021 gcactcagag tgtcgccaga cttaacggta taaaactggc tggcagtggc tggtgtcgcc
    10081 gttttcacct gatcatcaac actggcaata ccggaaatat tccccaccgc aacaaggatt
    10141 ttctccttcg cctcctgact caggccgtca ccagtgaccg tcgctttgcc gtcggcaatt
    10201 tgaatgttca ctttatcggc atcaggtata ccggttttgc tcagatgctc ctgcaccttc
    10261 ttcgcctgat cgtctttatc gtgctgacct gtaaccgcat cccagagttt ttctccggca
    10321 tctttaacaa aattgaacag acccatagct acctcattcg ttaacggaaa cactcagaaa
    10381 gtgtagcaga ggaacgcggc gcgttaacgc cgcgggggtg agcacaggtt taatcgcggg
    10441 tttgcaccca gaacgcgtga atcaaaccgg ggatataccc aagcagtgtc agcagaatat
    10501 taataatgaa cgcccaaccg aaccctttac cgagcagcac gccgatcggc ggcagaatga
    10561 tggtgatgac gattctccag aaacccatat gtactcccta taagaaaatt actcattgtt
    10621 taaaaagaga ttttatctct taagcgtagt aattttagcg gagcctgcca gt
//
LOCUS       DAEBXN010000129        10655 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.129, whole genome shotgun sequence.
ACCESSION   DAEBXN010000129 DAEBXN010000000
VERSION     DAEBXN010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10655
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.129"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(<1..1792)
                     /gene="selB"
                     /locus_tag="KJL99_003565"
     CDS             complement(<1..1792)
                     /gene="selB"
                     /locus_tag="KJL99_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HBD4438156.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALDIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHA
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTR"
     gene            complement(1789..3180)
                     /gene="selA"
                     /locus_tag="KJL99_003566"
     CDS             complement(1789..3180)
                     /gene="selA"
                     /locus_tag="KJL99_003566"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HBD4438157.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLVAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(3278..3886)
                     /locus_tag="KJL99_003567"
     CDS             complement(3278..3886)
                     /locus_tag="KJL99_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HBD4438158.1"
                     /translation="MKLVGSYTSPFVRKLFILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            complement(3957..5042)
                     /locus_tag="KJL99_003568"
     CDS             complement(3957..5042)
                     /locus_tag="KJL99_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBD4438159.1"
                     /translation="MDLLIVLTYVALATAALGGVFLVSGLILLMNYNHPYTFTAQKAV
                     IAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKT
                     LRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNFLAQDAL
                     VKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLI
                     RPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGK
                     LTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYS
                     DHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(5045..5407)
                     /locus_tag="KJL99_003569"
     CDS             complement(5045..5407)
                     /locus_tag="KJL99_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HBD4438160.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATEPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            5944..7857
                     /gene="mtlA"
                     /locus_tag="KJL99_003570"
     CDS             5944..7857
                     /gene="mtlA"
                     /locus_tag="KJL99_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HBD4438161.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGKRGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            7952..9100
                     /gene="mtlD"
                     /locus_tag="KJL99_003571"
     CDS             7952..9100
                     /gene="mtlD"
                     /locus_tag="KJL99_003571"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBD4438162.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            9100..9687
                     /gene="mtlR"
                     /locus_tag="KJL99_003572"
     CDS             9100..9687
                     /gene="mtlR"
                     /locus_tag="KJL99_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HBD4438163.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAVENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(9699..9908)
                     /locus_tag="KJL99_003573"
     CDS             complement(9699..9908)
                     /locus_tag="KJL99_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438164.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            10192..10554
                     /locus_tag="KJL99_003574"
     CDS             10192..10554
                     /locus_tag="KJL99_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HBD4438165.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTTF"
BASE COUNT         2559 a         2878 c         2750 g         2468 t
ORIGIN      
        1 gacgcgtaaa gccaatgcgg tcaaaatatt ccagaatctg aattgccagc tttcggccta
       61 cgcctaagcg atcgcggaaa tccgccgcgc aggttgaacc acactcctga tcgagatcgc
      121 ggatcatatt ggcaaattcg acaatccgat cgttacggta ataacgatct ttgacgatcg
      181 cggtaattat tccttgctgc gccgcctggc gtagcgtcag gcgcattgcc tgctcgtcgg
      241 tacccgtctc ttttgccaga tcacgcaccc accacggctc gtcaccaaac agcggctctg
      301 ctttttgcca gatggcctgc tgctcttcgc tgaagcccgc tttgtgatcc ggcagatgca
      361 gccaaccgtg atgactgagg atgtcgccgc tttcgcgcat cttttcaatc agcaacagta
      421 ctagcgcttc atcttccatt ggcaacgcca tacgtcgcag acgttcgcgc ccaggcccag
      481 gttcatcgcg atgttgctca tgataagtcg ctaatgtgtc gagaattttc cgttgccagc
      541 gagcggcaac cggcgcattc aacaagctat aaccagcctg aatatagcca ggctgttgca
      601 gcagttcgcg catcccttcg ccgttgagct ggcgcgccca agcgaaatca ggaagattaa
      661 ccgcgccgcg ttgcaggtga acggctaacg cttcgctatc attctgtgcc ctggcgaggg
      721 aagccagcca ttgcagatac tctggtttac gtttaccgcg acgcggcggg ttaagcatca
      781 cgacgcgcgc tccggccagc gtgttgcggg cagagatatc gcgcaatacc aggcggtcgt
      841 tatctgccag ccataacggg gtgtcgaaga ccagttcagc aaggttatct tccagcagtg
      901 aaacgcgtcc cgtgacgtgg ctggcggcgt ggtgaatatg cagcggctgc cactgggtca
      961 gcggtgcatg ggtttgaagc tcgacaatca cccgtgtgaa cggctctggc ggcacatcgg
     1021 caagcagcca gtcgccacgg ttaatctgct ctttttccgc atcacccgcg atgtcaagcg
     1081 cgatacgctg cccggcattg gcggtttctg ttggctggtt ttgcgcatgc agcgcacgta
     1141 cacgcatcgg tttatttaca ccagtcagcc agagtgaatc gcctaccttc acttccccgc
     1201 ttaacgccgt accggtgacg accagcccgg caccttttac ggtaaatgcg cggtcaatcg
     1261 cgaggcggaa actatgttgg ctggcgtgct cgcgttccgg caactgaagc agatgctcgc
     1321 gcagggcatc aattccccga ccttcggttg ctgcggtgat aaacagtttt gcctcagcaa
     1381 aaccgtattc ccgcagaacc tcctttacct ggcgttcaac ctcatcaaca cgcgcttcgt
     1441 ccacgcgatc ggctttggtc agcgccactg tcagcatcgg gttaccggtc agctgcaaaa
     1501 tcgccagatg ctcacgggtc tgtgccatca cgccgtcatc gcacgccacc accaacagcg
     1561 cgtgatcgat accaccaacg cccgccagca tgttggaaag aaacttttca tgaccgggaa
     1621 cgtcgataaa accaggcacg cgaccatccg gctgcggcca gtaggcatag ccgagatcga
     1681 tggtcatgcc gcgctttttt tcttccggca gacggtcagc atttacgcca gtaatcgcct
     1741 gcaataaggt tgttttgccg tggtcaacgt gtccggcagt cgcaataatc atttcaacaa
     1801 catctccaaa aaccgttgct catcttcaag gcagcgtaaa tccagccaca atcgtccgtc
     1861 ataaatacga ccaatcaccg gcactggcaa ttcacgccag cgggcggcta atgactcaag
     1921 gtggctaccg cgtccatcat ggggtgtaaa cgttaatgcc gcgctcggca ggcgatcaac
     1981 cggcagcgaa ccactgccaa tctgcgaaag acatggcata acctgtaccg caaactccgc
     2041 gccgtaatgt gcgacaaggg gagcctgtaa acgttgtgcc tggatttgaa tgacctctgc
     2101 gctgcgggta agcaggcgca gggtcggtaa tttttcactc agagcttcag ggtgtaaata
     2161 aagacgcaac gtggcttcca gcgccgcgag ggtcatttta tccgcgcgta atgcacgctt
     2221 cagcgggtgg ctttgcaggc gggcgatcat ctctttttta ccaacgataa tcccggcctg
     2281 cggcccgcct aacaacttgt cgccggagaa gctcaccaga ctgacgcccg ccgcaatcaa
     2341 ctcctgcggc attggctctt tcggcaaacc gtactggcta agatcgacca gcgagccact
     2401 gcctaaatca gtcaccactg gaacatccag ctctttgccg agcgccacca gttcagcttc
     2461 gtctatcgct ttggtgaacc cctgaatgct gtagttactg gtatgtactt tcatcaacag
     2521 tgcggtattt tcattcaccg cctgacgata atcattcgcg tgcgtgcggt tggtggtccc
     2581 tacttcgtgt agggtgcagc ctgcctgacg cataacatcg ggaatacgaa acgcgccgcc
     2641 aatctccacc agttcgccgc gagataccac cacctctttc ccgctggcag tggccgccaa
     2701 catcaataac accgccgccg cattgttatt gacgatacag gcatcttccg cccccgtaat
     2761 acggcacagc agctgcgcca gcgcccgatc gcgatgtccg cgtccggcgt cgtccagatc
     2821 gtactcgagg gtcactggcg aacgcatagc ctgcgcaacg gcttccaccg cggcttccgc
     2881 ctgtaaagct cgcccaaggt tggtatgcag cacggttccc gtcaggttga tcaccggacg
     2941 cagcgcgctc tgcgcctctt tcgtcaaccg ggcatcgact tcttgcgccc agttttcaca
     3001 ccacgcaggc agcgtctggc tgccacgaat cacttctcgc gcttcgtcga gcatctgacg
     3061 caacaattcc accacgcggg tgtgaccata agtatcacgc aaagaaagga aggagctatc
     3121 gcgcaataag cgatcaatag ccggaagttg actatagagg gaacgcgttt cggttgtcat
     3181 aggaaacctg gctgatcaag gccctctcac acggagaagg gcgtttaaca taaccacgga
     3241 ttgtaacgtg agatgggtca ggaggacata tcgcgcatca agcctttggc ggttcggtgc
     3301 gagcaaaact ttcgcggcta aacaggtttt cgaccagttt gactaagtgt gggcggtcga
     3361 cacaccaacc tggcgcaacg cggcggaaat tgagatatcc gacagcacag gcaatggcga
     3421 tagtcgccag attgaccgta tcggttttga gtgtgccatc gaccagatat ccttccagca
     3481 catccagact gcggttgatt ttctcccgct ggcgtaacaa ttcatcttca gactgctgcg
     3541 ccgccggacg cgcctgttca cgcaccgata ccagcccggc atccataatg ccatccgcca
     3601 gcgcctcaat tttgcgcacc cgcaacgact ccagcggatc gcgcggcaac atcgccggag
     3661 cgacattcat taactcaata tattcagcga tgatcggcga atcaaaccag cattcgccct
     3721 cttcggtcac cagcaccggc actttcccta acgggttaaa ttgcgccacg ccgttatccg
     3781 cgttataggg cagttcatta ataaattcga aagttatgcc cttttctaac aacagaataa
     3841 aaagtttgcg tacaaacggg ctggtgtagc taccgacgag tttcatgccg agtcctttgt
     3901 gcgaggaaaa atatcagtat ggctcaggct cacgcaaaac acaggtgata taagtctcaa
     3961 tgatccaaat aaagataatg catccaactg gtcattctta gcagcacttt ccgcatcacc
     4021 gaaacatggc tgaaatggtc ggagtaaacc gccacctggg cgtagatgcc gtcgggtaag
     4081 gcatcgatat catcgttagg gtccagttca atggttccca gcacaccgtc cgtgccggga
     4141 acgaccgtta atgattgcaa taccccctgc gcctgataag aaccgcctgg cacgacaggt
     4201 aaaatactag tcaatttgcc gtggaacacc tgcccaggta gcgcgttaaa caccacttcc
     4261 gcatcatcgc caggtttcag acgtaacagc gagttttgcc gaaattgggc gacaatttgc
     4321 cgtttttgct cggggatgaa gaccatcacc ggacgcagcg gcaaggcagc tgcgtatgta
     4381 cctgggcgga tcagtacctg agtgacgtag ccattgcttg gtgcgcgaat gacagtctgc
     4441 tcaagattat attttgcttc agtaagttgc gctcttaagc tcacaatctg cgattgctcg
     4501 ccgttgacca tactgtcgag ctgactctgg atctgcgcct gctccgccac cgagcctttc
     4561 actaacgcat cctgcgcgag gaaattttgc cgcgcatcgt cgatgtcacg ttccgagaac
     4621 ggattcaccg ccgcctggct gcctttcagg taacgttgat aatttttaaa cagacggtcg
     4681 cgctccgctg aaacctgggt ggtgttggcc tgcgcttcag tgagctgcgc acgcagcgtc
     4741 tttatattat gcgtcgccgt catcaggtca gcctgaagtc tgtcaactcg cgcctggtaa
     4801 cgaaccgggt cgagcttaaa aagcacctcg cccttttgaa taagctgatt attcttgtca
     4861 gtgacttcag taacaattcc cgtcacctgt ggcgtgatag ggatcgctat cactgccttt
     4921 tgcgcggtaa aagtgtaagg gtggttgtag ttcatcaaca aaatcaaacc actcaccaga
     4981 aacacgcctc ccagcgccgc cgtcgccagc gccacgtaag ttaaaacaat caatagatcc
     5041 ataattagat ctccgggaac gtggaatagt cagtcttttt ctcggcggcg agttgatgct
     5101 ccagccggga aattcggtca gaaagtgcgg cgatttccgg ttcagtcgct ttctcctgcg
     5161 acgcaacatg tgactgcata ccccagccac gctccggttg atagagcgtc gcccagatcc
     5221 acagaaacgg ccagataaca tgcagagtaa acaggctcac ccagcccgcc gtatgaatag
     5281 cgtcggcatg ggggtgattg cgctttttgg caatcagata agggatgtca tgtatcgcga
     5341 tgattccata aaaaatcacc agaaagacga agatcagcac tcccaacgcg aaatagttta
     5401 gaaacatatc tgcctcggat tcacgtttat cagtgttgtt ttttggctgg cagccagaag
     5461 ggagtcaggc tgatattttg acaataatcc gggttcgcga ttctcgccat aacaccaaag
     5521 aataattttt agaggtgatg agttgcttag ttacataacg attgtatgac gaaggcataa
     5581 catgctgtag atcacatcag gtgaacgccg taagaaaata tcttgtgatt cagatcacaa
     5641 agattcaaca aaccatcaaa acaaaaatgt gacattactc acatttaaat gccattttta
     5701 gcgaaaatca ccgccttgtt tcttttttat acaggcgttt tgtgatgagc gtcacgtcaa
     5761 ttacctctct accccctata tttatgtgac tgatatcaca caaaagcccg tcgactggac
     5821 agttaaccga ttcagtgcca gatttcgcag tatctacaag gtccggctac ctctgccgcc
     5881 acattaacaa aaaacctcgg gcttccagcc tgcgcgacag caaacataag aaggggtgtt
     5941 tttatgtcat ccgatattaa gatcaaagtg caaagctttg gtcgtttcct cagcaacatg
     6001 gtgatgccaa atatcggcgc gtttatcgcg tggggtatca tcaccgcgtt atttattcca
     6061 acagggtggt taccgaacga gacgctggcg aagctggtcg ggccgatgat cacttatctc
     6121 ctaccgctgc tgatcggtta taccggtggt aagctggtag gcggcaaacg tggtggcgta
     6181 gtcggtgcca ttaccaccat gggcgttatc gtcggcgcag acatgccgat gttcctcggt
     6241 tctatgattg caggtccgct gggcggctgg tgcattaagc acttcgaccg ctgggtagac
     6301 ggtaagatca aatccggttt tgagatgctg gtgaataact tctccgcagg catcatcggg
     6361 atgatcctcg ctattctggc attcctcggc attggcccga ttgttgaagc cctgtccaaa
     6421 atgctggctg cgggcgttaa cttcatggtt gtccatgaca tgctgccgct ggcgtctatc
     6481 tttgttgaac cggcgaaaat cctgttcctc aacaacgcca ttaaccacgg tatcttctcg
     6541 ccgctgggta ttcagcagtc ccatgaactg ggtaaatcaa tcttcttcct gattgaagct
     6601 aacccaggtc caggtatggg cgtgctgctg gcgtacatgt tctttggtcg tggtagcgct
     6661 aaacagtctg cgggcggtgc ggcaatcatc cacttcctgg gtggtatcca cgaaatctac
     6721 ttcccgtatg tgctgatgaa tccgcgtctg atcctcgcag tcatcctcgg cggtatgact
     6781 ggcgtgttca cgctgactat cctgggcggt ggtctggttt ctccggcatc tccgggttct
     6841 atccttgctg tactggcgat gacaccaaaa ggtgcttatt tcgctaacat cgcgggtgtg
     6901 tgtgcggcga tggctgtctc cttcgttgtc tctgctattt tgctgaaaac cagcaaagtg
     6961 aaagaagaag atgatattga agcagcaact cgtcgtatgc aggacatgaa agctgagtct
     7021 aaaggcacat ctccgctatc tgcgggcgat gtgactaacg acctgagcca cgtacgtaaa
     7081 atcatcgttg cctgtgacgc cggtatgggt tcaagtgcga tgggcgcagg cgtgctgcgt
     7141 aagaaaattc aggatgcagg tctgtcgcag atttcggtca ctaacagcgc gatcaacaac
     7201 ctgccgccag atgtggacct cgtcatcact caccgtgacc tgaccgaacg cgctatgcgc
     7261 caggttccgc aggcacagca tatttcgctg accaacttcc tcgacagcgg cctgtacacc
     7321 agcctaaccg aacgtctcgt tgctgcccag cgccatactg aaaacgaagt gaaagtgaaa
     7381 gacagtctga aagacagctt tgacgattcc agtgctaacc tgttcaaact gggcgcggag
     7441 aacatcttcc tcggtcgcaa agcagcaacc aaagaagaag cgattcgttt tgctggcgag
     7501 cagctggtga aaggcggtta cgttgagccg gaatacgttc aggcgatgct ggatcgtgaa
     7561 aaactgaccc cgacttatct gggtgagtct atcgcggtgc cacacggtac ggttgaagcg
     7621 aaagatcgcg tactgaaaac gggcgtggtg ttctgccagt acccggaagg cgtgcgcttc
     7681 ggtgaagaag aagatgacat tgcccgtctg gtgattggta ttgcagcccg taacaacgag
     7741 cacattcagg ttatcaccag cctgaccaat gcactggatg atgagtccgt catcgagcgt
     7801 ctggcacaca ccaccagcgt ggatgaagtg ctggaactgc tggcaggtcg taagtaatcc
     7861 aatcctaccc tctcctcacg gagagggttt gggtgaggga aaagcctcac cccagccctc
     7921 tcgggtaaaa acattgatga aggttaatac tatgaaagca ttacattttg gcgcaggtaa
     7981 tatcggtcgt ggctttatcg gtaaactgct ggcagacgcg ggtatccaac tgacgtttgc
     8041 cgatgtcaat caggtggtac ttgatgccct gaatgcccgt catagctatc aggttcatgt
     8101 ggtcggtgaa accgagcagg tagataccgt ttccggcgtc aatgctgtca gcagcattgg
     8161 tgatgatgtc gttgatctga ttgctcaggt cgatttagtc actaccgccg ttggcccggt
     8221 tgtgctggaa cgtattgctc cggctatcgc caaagggctg gtgaaacgca aagaacaagg
     8281 taatgaatcc ccgctgaaca tcatcgcctg tgaaaacatg gtacgcggca ccacgcagct
     8341 gaaaggtcat gtgatgaacg ccctgccgga agacgccaaa gcgtgggtag aagagcacgt
     8401 tggctttgtc gattccgccg ttgaccgcat cgtaccgcct tcggcttcgg caactaacga
     8461 tccgctggaa gtgacggtag aaactttcag cgaatggatt gtcgataaaa cgcagttcaa
     8521 aggcgcactg ccgaacatcc caggcatgga gttaaccgac aacctgatgg catttgtcga
     8581 acgtaaactc ttcaccctga acacgggtca tgctataacc gcgtacctcg gaaaactggc
     8641 cggtcatcag accattcgtg acgctattct cgacgagaaa atccgcgcgg tggtaaaagg
     8701 tgcgatggaa gaaagtggtg cggtactgat caagcgctac ggctttgacg cagacaaaca
     8761 tgcggcgtac atccagaaaa tcctcggtcg ttttgagaac ccgtatctga aagatgatgt
     8821 agagcgcgta ggccgtcagc cgctgcgtaa actgagtgct ggcgaccgtc tgatcaagcc
     8881 actgctcggt acgctggaat acagccttcc gcacaagaat ctgattcagg ggattgctgg
     8941 tgcaatgcac ttccgcagtg aagatgatcc gcaggctcag gaactggcag cactgatcgc
     9001 tgacaaaggt ccgcaggcgg cgctggcaca gatttccggt cttgatgcca acagcgaggt
     9061 tgtatccgag gcggtaaccg cttataaagc aatgcaataa tggtggacca ggcgcaggac
     9121 accctgcgcc cgaataacag attgtcagat atgcaggcaa caatggaaca aacccaggcc
     9181 gttgaaaacc gtgtgcttga gcgtctgaat gctggcaaaa ccgtgcgaag ctttctgatc
     9241 accgccgtcg agcttttgac cgaggcggta aatcttctgg tgcttcaggt attccgcaaa
     9301 gacgattacg cggtgaagta tgctgtagaa ccgttactcg acggcgacgg tccgctgggc
     9361 gatctttctg tgcgtttaaa actcatttac gggttgggcg tcattaaccg ccaggaatac
     9421 gaagatgcgg aactgctgat ggcattgcgt gaagagctaa atcacgacgg caacgagtac
     9481 gcctttaccg acgacgaaat ccttggaccc tttggtgaac tgcactgcgt ggcggcgtta
     9541 ccaccgccgc cacagtttga accagcagac tccagtttgt atgcaatgca aattcagcgc
     9601 tatcaacagg ctgtgcgatc aacaatggtc ctttcactga ctgagctgat ttccaaaatc
     9661 agcttaaaaa aagcctttca aaagtaagca acgtctgctt actgcccctc tacctgcttc
     9721 ggccgataaa gccgacgata atactccaga cgttgtagat ataacggcac gctttcatcg
     9781 ggtattccgg acggaatcgc gttacgggga ggaagttttt tcagatactc ccggaacgcc
     9841 tggcttgatg ccatgaaatc tacggcttta tcgataagaa gcggaacgtt ttcacctaat
     9901 ttgcccatga tgatatctcc gtattacccc gcgccgggaa tgcgcggccg ccaattttag
     9961 tttaggttcc cggtaaagat gacattaagg aaaacgtgct gaatcctcaa taatcatccg
    10021 ctgctgttta ttatcttttt ctatagattt cttaattaat caacgaattg cgtagttttt
    10081 gagcatgtta atgattacgt tgcagaacta ttacaatgtc atcagttgtg aacaaagcac
    10141 ctggtcgcgc atactagggg ctataaattt atctttatca gaagccatca catgaaagaa
    10201 gtcgaaaaaa acgaaatcaa gcgtctcagc gatcgcctgg acgccatccg ccaccagcag
    10261 gccgatctgt cgctggttga agccgcagac aaatatgccg agctggaaaa agagaaagcc
    10321 acgctggaag cagaaattgc tcgcctgcgt gaggttcata gccaaaaact gagtaaagaa
    10381 gcacagaagc tgatgaagat gccgttccag cgcgcaatta ccaaaaaaga gcaggctgat
    10441 atgggcaagc tgaagaaaag tgttcgcgga ctggtggttg tgcacccaat gaccgcgctg
    10501 gggcgcgaaa tgggcctgga agagatgact ggattttcta agactacgtt ttaagcccct
    10561 cagacgactg gttcgatcgt atcaacccgc atctggcttg ccttagctgg tcaatgcggg
    10621 gactctcctg tctactcgcc taattaccaa acaat
//
LOCUS       DAEBXN010000130        10646 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.130, whole genome shotgun sequence.
ACCESSION   DAEBXN010000130 DAEBXN010000000
VERSION     DAEBXN010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10646
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.130"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(386..820)
                     /locus_tag="KJL99_003575"
     CDS             complement(386..820)
                     /locus_tag="KJL99_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HBD4438166.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSRAWGRQEVPSEDARADNPLQRL"
     gene            complement(808..1209)
                     /locus_tag="KJL99_003576"
     CDS             complement(808..1209)
                     /locus_tag="KJL99_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438167.1"
                     /translation="MYAKSFIALDGNGRLTGARTAQAAPYANYTCHLCGSALRYHPQY
                     ETELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGGFSIPRTTQEEICEF"
     gene            complement(1469..2038)
                     /locus_tag="KJL99_003577"
     CDS             complement(1469..2038)
                     /locus_tag="KJL99_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HBD4438168.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVN
                     RDTWCRAGDYRAPESLAGMIKQAWCSALGVDVGCHATLVHFPAWPAVWLARNDDTGFQ
                     QVLERADYLAKEHTKAHCTGERNFGCSRS"
     gene            complement(2783..2926)
                     /locus_tag="KJL99_003578"
                     /pseudo
     CDS             complement(2783..2926)
                     /locus_tag="KJL99_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin activation protein"
     gene            complement(3260..3526)
                     /locus_tag="KJL99_003579"
     CDS             complement(3260..3526)
                     /locus_tag="KJL99_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438169.1"
                     /translation="MAEPYRYTGWLLRKDASARTRDKEGIVFVRTGREAGVLIIAELF
                     SEVVTVSQPVPGEEETDITDGIRLSDWYYKYKKSSESNVKDLPE"
     gene            3667..3873
                     /locus_tag="KJL99_003580"
     CDS             3667..3873
                     /locus_tag="KJL99_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HBD4438170.1"
                     /translation="MATPVSLMDDQMVDMAFITQLTGLTDKWFYRLIRDGAFPAPIKL
                     GRSSRWLKSEVEAWLQARIAQPRP"
     gene            3968..4570
                     /locus_tag="KJL99_003581"
     CDS             3968..4570
                     /locus_tag="KJL99_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438171.1"
                     /translation="MKQQYQTRYEWLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRATPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSLEDWKENLKRK
                     SEKELEEWIINRQRQSAALTDLMDQYVLLAYRTTVDDSRN"
     gene            5770..6687
                     /locus_tag="KJL99_003582"
     CDS             5770..6687
                     /locus_tag="KJL99_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438172.1"
                     /translation="MVQKILSDKVMNERTNAYYSYYLGERNISVLPLNVYDPPERFIA
                     YIKKNRENLNITLSDFELEQIISGMRLKALAFLVPLEKISWIAGSERACLFSWYLLMQ
                     FIQNNRAKISADLLQKNKLYLKEEYLEGNAFPSDSSTQFRQILRVLDILSDKNLRDEW
                     IIQTKDRWMRAFKSKSPFSYLLPENEHECIWTWNYLKGKNIALEKLASFPGSADIYHA
                     IHLSFDIWVTCPLTSPDDIKNFRNSFNKAKAQRKYKKMQEDKVNVQFFLDAETRAQLK
                     ELSRVRRLSTGEMLHDLIVEEYKRYRHSR"
     gene            6772..7644
                     /locus_tag="KJL99_003583"
     CDS             6772..7644
                     /locus_tag="KJL99_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HBD4438173.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRGQAREQFTGAVDRIFDTAESVCVASVARTVLRAVRDT
                     VVSVARAVWNWIFF"
     gene            8016..>10646
                     /gene="ag43"
                     /locus_tag="KJL99_003584"
     CDS             8016..>10646
                     /gene="ag43"
                     /locus_tag="KJL99_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HBD4438174.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVVLSLAA
                     VTSVPALAADTIVQAGETVNGGTLTNHDNQIVLGTANGMTISSGLEYGPDNEANTGGQ
                     WIQNGGIANNTTVTGGGLQRVNAGGSVSDTVISAGGGQSLQGQAVNTTLNGGEQWVHE
                     GGIATGTVINEKGWQTVKSGAMATDTVVNTGAEGGPDAENGDTGQFVRGNAVRTTINE
                     NGRQIVAAEGTANTTVVYAGGDQTVHGHALDTTLNGGYQYVHNGGTASGTVVNSDGWQ
                     IIKEGGLADFTTVNQKGKLQVNAGGTATHVTLKQGGALVTSTAATVLGSNRLGNFTVE
                     NGKADGVVLESGGRLDVLEGHSAQKTRVDDGGTLAVSAGGKATGVTMTSGGALIADSG
                     ATVEGTNASGKFSIDGTSGQASGLLLENGGSFTVNAGGLASNTTVGHRGTLTLAAGGS
                     LSGRTQLSKGASMVLNGDVVSTGDIVNAGEIRFDNQTTPDAALSRAVAKGDSPVTFHK
                     LTTSNLTGQGGTINMRVRLDGSNTSDQLVINGGQATGKTWLAFTNVGNSNLGVATSGQ
                     GIRVVDAQNGATTEEGAFALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYTS
                     MLTQAMDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGSY
                     GFVRLEGDLLRTEVAGMSLTTGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNL
                     THTSSGLWADIVAQGTRHSMKASSDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQL
                     HYTWQGLTLDDGQDNAGYVKFGHGSAQHVRAGFRLGSHNDMSFGEGTSSRDTLRDSTK
                     HRVSELPVNWWVQPSVIRTFS"
BASE COUNT         2559 a         2774 c         2749 g         2564 t
ORIGIN      
        1 ttcgcctgcc gggagtcaca tttttctgca tatctgacac tttggttccc actacttatt
       61 tggtggacac cactttgtct aattcgtcag attctgacca gacggttcag gctgtacgct
      121 taccactatt ttatccctgt tgaatttggt taagccggtg cagacgcgcc gggaaacgca
      181 cacctgaacc ggcacaataa aatacaatac aaccccctgt tactggagat acgaatggaa
      241 tgacgaggca gcctgtcagt cacagggggc tgttggcata aaatcaacgg aaaggccacc
      301 aacaccggaa ttaagctcca cagtccccgc actggcaatc cattcccggg aaaggcatca
      361 ccgcaaactg ctccggaggc aaccattaca gcctctgtaa cggattatca gccctggcat
      421 cttccgacgg cacctcctgc cgcccccacg ccctgctgta taccccggta ccgcagcgtg
      481 ggcagcactt ttccccctca taatcttcgt cacacatcac acaaaaccac tttctcacct
      541 tcaccgtata atccggaacc gtcatgccct gcagatgcaa acgacgggcc tctttctctg
      601 ccgggtcaag ccaggtacat cgcagcagga ttttttcagc gacagtcatc tggtcatgtt
      661 caaaccacgg tggttgtccg tcattgcccc gctgaagtgt taacgggcat ccacaggaca
      721 cacagcacaa aacgtcctgt cggttgtgct tcgcagcttc tttcgccgta acaaaatggc
      781 ctttgctgtc attcgccata taacagttca gaattcgcat atttcctcct gagttgtccg
      841 gggaatactg aatcctccgg tctggcagtg tgtgcagtac tgctccccat aataatcgtg
      901 gtgacattgt ctgcagtgcc agctggcttt acgcaccacg ggtaaggcat ccggtacgaa
      961 ttgctgcaga cgtttaatca actgtatttc tctgcgctcc ggcctgacat aagggcactg
     1021 ttgaccgtgc tctgtcagcc tgtcgtcagt gtgttcaaac cagggaagtt cagtttcgta
     1081 ttgcggatgg tatctgagtg cactgccaca caagtggcag gtgtagttag cataaggtgc
     1141 ggcctgtgca gtacgggcgc ccgtcagacg tccgttgcca tcaagagcga taaaggattt
     1201 tgcgtacata gtcatatacc tcagccgcca gacgacacgc aggcaacagc cgtcccggta
     1261 cgggcagcgt ggtcagggtg tgaatggcgg ttcagttaaa atctggactg gagtttgggt
     1321 aaagcaggct ggttgctgtt acagcactgt ggttgtcaca caatggaaca ctgacgtaaa
     1381 aaacagtcag tgggcgagtg gatacatcgt tgccgccaga caacaaccca tacttttggt
     1441 ttgccggata ttggcgcgcc agtcgtgctc aactccggct acagccaaaa ttgcgctcac
     1501 cggtgcagtg agctttggta tgctccttcg ccagatagtc agcacgttcc agcacttgct
     1561 gaaagccggt gtcatcatta cgcgccagcc acaccgccgg ccaggccgga aaatgcacca
     1621 gcgtggcatg gcacccgaca tccactccca gggcactgca ccacgcctgt ttaatcatcc
     1681 cggccagaga ctccggagcg cggtaatcac cggcacgaca ccaggtatcc cggttgacca
     1741 gcagcatcag gtgatagtgt tttttacctt tgcactcccc aaactcccgg gcccagacgt
     1801 aatgcagggt tgtgggatgc acgcgtttac cttcccgatg cttacgtttc tggtaagcgt
     1861 cgattcgggc tttcagggca ttgatgaagc gggatatcac agctgcgtcc gttgctgccg
     1921 gtacatccgg gagacgcagg tcaacccgca atgccgtcag gcggggatga acattcagtg
     1981 cgtgccgcac cgtctcacga atacgttgct gccagaaggg gttgtatttg taggtcatgg
     2041 ttaaatctcc gtatggttca tacggaatag ccacgtcgta aaaaatacgc agagtccctg
     2101 aagtgacctc cggcaggaca ctgtctcagg cactctgtga taacccgaac ggtcgtctcc
     2161 ggactgacgg ttgaattttc agcgtcgttt tttaatgtaa aaaaccggtt acgggtaagg
     2221 ctgtgaggag ggagtgatgg tgatacgcaa aaagaagtgc agggactgtg gaaacgcgat
     2281 tactcataat acagtgtgtt gcccgtattg tggttctgtc gaccccttcg gctactaccg
     2341 gaatacagac cgtatcgtta ccatactgct ggcgctgatt attgtggtcc tgctgactac
     2401 tgtcagcgtc agtgtgtgta tactgtgtag ctggtgagat aacggttcat gtatcccctg
     2461 agctgacagt gtaccgtgta tattaagcgt aaccgacagg ttctccttca taccatatta
     2521 aattttccag ggattgccgc tgccttattt cgtcccatac aggaaaactc atctgggttt
     2581 ctgacaaata cgcgaaactc acagcccctc aatatcaacc acacgttgta agccggtaat
     2641 acctgaaaat cagcctatta ccgcccacgc ctccggcacc attccctttt gatttcgaat
     2701 tctttgttgt cgctataccg cttaatgttc ccggtcctcc ggtgcagata acaccggagt
     2761 catcgtagcg ctgcttcaat attcaggtga cgtatttcca gacggaagcc ggcgcgcgat
     2821 cgtaaagttt gttcattgtc agttctgcca gccggttgtc aacggcagag ttaaaggctt
     2881 ccagctccgc aagggtaagt ttataacgcg tgtgggaatt cacttttccc gcgtctcctg
     2941 acgtattacg ggctgccact tacctggtgt cccgcaatac agccttagtg ttccgttatc
     3001 ggggatccgg acgcaccgca acaaccgggg aagtatttct ggtttagagc agtgtcaaga
     3061 gggatttctc agcccctcac tcaccggctc agtctcaggg caggagcatt aatacgctca
     3121 gttcatgtct tatacaggaa tgggcacagg gggagctggc gtgtcactgc tgattttacg
     3181 catcagcgtt catgtatctg cactctgata catgaacgat tcacagcgct ctgttaccgg
     3241 agagatttac tctcttagct tactctggca aatcctttac gttactctct gatgacttct
     3301 tatatttata ataccaatca gacagcctta tcccatcggt gatatcagtc tcctcctcgc
     3361 caggaacggg ctgactcact gtaactacct ccgaaaacaa ctctgcaatt atcagaacgc
     3421 ctgcttctct ccctgtcctc acgaaaacta tcccctcttt atcgcgcgtg cgtgcggaag
     3481 catcttttcg caacaaccac ccggtatacc ggtacggctc agccaccgca acctccccgt
     3541 ttatctcctg gcggtcacat tcccgattat tttttacgtt tctccccggt tgttatgccg
     3601 gtgaaggtgg cgcgctgttt tcatgaccac accggctggt ttaacaacat ccggaggaac
     3661 attctcatgg ctaccccagt ttcactgatg gatgaccaga tggtcgacat ggcatttatc
     3721 actcaactga ccggcctgac agataagtgg ttttacaggc tcatcaggga tggggccttt
     3781 ccggctccca tcaagctggg ccgcagctcc cgctggctga aaagtgaagt ggaagcctgg
     3841 ctacaggcgc gtattgcaca gccccgcccg taatttctga ccgttatccg ttcacccgca
     3901 gcagacgtaa tccccggcct gccggcggca ttctgctgcc tgttatatcc ccgtgaggaa
     3961 tatgaaaatg aaacaacagt atcagacccg ctacgaatgg ctccacgaaa gctaccagaa
     4021 atggctgacc ggcttcaccc ggcacgccgt atcctggggc gtgtgtcatc cgaatatcta
     4081 ctacttccat aatctgacgc ccggttgggt gtcattcaat ggtgaaaaac cggagattgc
     4141 catcgtaccc cagagcctgc accggctgat ttatggccct gacaaacggg ccacgccgcc
     4201 cctggatgat gatctgatag tgaatttatg caccagtgaa catctgctgg ttcatcatcc
     4261 gatgctggaa ggcattctcc tgtctgaatg tgaacgtctg aggcagcgtt ctctggcaaa
     4321 taaactgatc agcctcttcc gtcagtttgg tggcacggag ttgcgcctca aactggtctg
     4381 gctttgctgg cttgatttaa tgaccggaaa cagccttgaa gactggaaag agaacctgaa
     4441 acggaaatca gaaaaagagc tggaggaatg gatcatcaac cgtcagaggc agagcgcagc
     4501 actgacggat ctgatggatc agtacgtgct cctggcatac cgcacaacgg ttgacgatag
     4561 ccgcaactga tgccggttgc ttccggaagg tgtgacaaaa tacggaaatg atgatgctcc
     4621 cggcccccat gctcacctgc agagcgtgtt cgtcgtcatc atactgagcc atgacgggcg
     4681 tcttaagggc atcctgtcat tgctgagcag gcaacaccac cagaggagcc acggacaggg
     4741 atgttcttta cagcccgata cacgccaggt tctcagcaga ctgaatgtgc aggtgcggtt
     4801 attcaccaga aatggcatgc tgcggtacat acccatcctg gtgacaatat gtacgtggcg
     4861 acagcgatcc cggcactgtg gaagagactc actcagcctg caggatattc tttccggcat
     4921 ccgggaatgt ccgcctgaat gcaaacagga tgatatttga atccgtaata ctgttctttc
     4981 atcataaata atttatgcag atacagttaa ccgttacagt agccggtttg ttttaccggg
     5041 aaataatatc cgtcagactg ataccggaac atatctgaaa cacgttatga tgttttgtta
     5101 tcagaaatgt tgtatattct tatccgtggc gacagtttaa tgccctctgg tatcacctga
     5161 taatgtatat cctgcgatga ttcgttatat ttacgggaca cgcctctttt cattgtgctc
     5221 cttttcagac ttcattttcc gtaagtgttc cccggcataa aggaggtacc tttctggcgt
     5281 ttataactct ctttatcggt gacgccggta atacagggaa taccacaccg acaaacgagg
     5341 tattaacaga cgatatttca gtgtccataa tttttctgtc cggttaaata acgtcagtct
     5401 gctgccctct gactgtaatc atcaaagccg gaatgcatac cgggttacaa cgtaaaaaaa
     5461 gtgatgctgg cccccggtgc agctggcggg cgaaacatgt ttacctttca ataccccctg
     5521 tctgcagtct ccactcttcc atcctcttaa gctacgtcgg ccacatgagt gcacttccat
     5581 gataacaggt attcctgtca gcataacgaa gccggttacc ttcccttcct cctgcatttc
     5641 ctgtcagaat gactatacag cgcgcagatt gcctttttat tgaccatctt aatataccgt
     5701 tataaacttc tttttatcat attattatca ccttattatg tttaattttt aagcagtcgg
     5761 agaaagagga tggttcagaa aattttgtcg gataaggtta tgaatgaaag aacaaatgcg
     5821 tattactctt attatcttgg agagcgtaat atctccgtac tgccccttaa tgtttatgat
     5881 cccccggaac gttttatcgc ttacataaaa aaaaacagag aaaatctgaa catcacactt
     5941 tcagattttg aacttgaaca aataatttct ggtatgcgtc tgaaggccct ggccttcctg
     6001 gtccccctgg aaaaaatatc ctggatcgcg ggcagtgaac gcgcatgctt attttcctgg
     6061 tatctgttga tgcagtttat ccagaataac agggcaaaaa taagcgcaga cttgcttcaa
     6121 aagaataaac tgtatcttaa agaagagtat ctggaaggga atgccttccc gtcggactca
     6181 tcgacacagt tcagacaaat actcagagtt ctcgatattc tttctgataa gaatttgcgg
     6241 gatgagtgga taatccagac caaagacaga tggatgcggg catttaaatc aaagagtcct
     6301 ttcagttacc tcctcccgga gaacgaacat gaatgcatat ggacatggaa ttatttgaag
     6361 ggaaaaaata ttgcactgga aaaactggcc agttttcctg gttctgctga tatttaccac
     6421 gcaattcacc tttcatttga catatgggtt acctgtccac tcacctcacc tgacgatata
     6481 aaaaatttca gaaacagttt caataaggcc aaggcgcagc gcaagtacaa aaaaatgcag
     6541 gaagataaag tcaatgtaca gttttttctt gacgcagaaa ccagggcgca gctcaaagaa
     6601 ttatcgaggg tcagaagact cagtaccggc gaaatgctgc atgacctgat tgttgaagag
     6661 tataaaaggt accgacattc cagataattt cagacatata ttatctccgt gagtcccccg
     6721 ccacctttcc ggtgtgcggg ggttttgtct tttttcaccg ggaatacatg tatgaatccg
     6781 tctgatgcca ttgaggcaat tgaaaaaccg ctctcctccc tgccttactc actttcccgt
     6841 cacatcctgg aacatctgcg caaactcacc agtcacgaac ccgtgattgg cattatgggt
     6901 aaaagcgggg ccggtaaatc ctcactctgt aatgcactgt ttcaggggga ggtcaccccg
     6961 gtcagtgatg ttcacgccgg cacccgggaa gtgcagcgtt tccgtctgag tggccatggt
     7021 cacagcatgg ttatcactga cctgcccggg gtgggcgaga gccgggacag agatgcagag
     7081 tatgaagccc tgtaccgtga cattctgcct gaacttgacc tggtactgtg gctgattaaa
     7141 gccgatgacc gtgccctgtc tgtggatgag tatttctggc gacacatcct gcaccgggga
     7201 catcagcagg tgctgtttgt ggtgacgcag gccgacaaaa cggagccctg ccatgaatgg
     7261 gatatggccg gtattcagcc ttctcccgca caggcacaga atattcgcga aaaaacggag
     7321 gcggtattcc gtctgttccg gcccgtacat ccggttgttg ccgtatcggc ccgcaccggc
     7381 tgggaactgg atacgctggt cagtgcgcta atgacagcac ttcccgacca tgccgccagt
     7441 cccctgatga cccgcctgca ggacgagctg cgcacggaat ctgtccgggg tcaggcccgt
     7501 gaacagttta ccggtgcggt ggaccggata tttgacacgg cggagagcgt ctgtgttgcc
     7561 tctgttgccc gtacggttct gcgtgccgtc cgtgacacgg tggtctctgt tgcccgcgct
     7621 gtatggaact ggattttctt ctgaacagtc tgccagggaa atgcctgata acattacgtt
     7681 aaccaactga aaacatgaca caaacccctc atactcctcc tgtttctctt gcgtgaccgc
     7741 cttgtcgtta atagaataaa actatcgata aaacagatat cgatcgttgc tatcgatcgt
     7801 ttatagtggt taatggtctg cgaaaaggca gctgaatctc ttcatcatgc agaacggaat
     7861 tgcacacaac ggactgatac ttctctggcg gatgagagag gggaagcatt tatgtccggg
     7921 aaaaaccact ttatctgacc atgctgtttt gtacctgccg gtatccactt ttgtgggtac
     7981 cggctttttt attcaccctc tgtaaggaaa agctgatgaa acgacatctg aacaccagct
     8041 acaggctggt atggaatcac attacgggca ccctggtggt ggcttccgaa ctggcccgct
     8101 cacggggaaa acgcgccggt gtggcggttg tgctgtctct tgctgctgtc acatcagtcc
     8161 cggcactggc tgctgacacg attgtacagg cgggagaaac cgtgaacggc ggaacactga
     8221 caaatcatga caaccagatt gttctcggta cggccaacgg aatgaccatc agtagcggtc
     8281 tggagtatgg gccggataac gaggccaata ccggcgggca atggatacaa aatggcggta
     8341 tcgccaacaa cactactgtc accggtggtg gtcttcagag agtgaatgcc ggaggaagcg
     8401 tttcagacac ggttatcagt gccggaggcg gacagagcct tcaggggcag gcagtgaata
     8461 ccactctgaa cggcggtgag cagtgggtac atgaaggcgg gattgcaacg ggtaccgtca
     8521 ttaatgagaa gggctggcag accgtcaaat ccggcgcaat ggcaaccgac acggttgtga
     8581 ataccggcgc ggaaggggga ccagatgcag aaaatggtga taccgggcag tttgttcgcg
     8641 gaaatgccgt acgtaccact atcaatgaaa atggtcgtca gattgtggct gctgaaggaa
     8701 cagcaaatac cactgtggtt tatgccggcg gcgaccagac ggtacacggg catgcgctgg
     8761 ataccacact gaatggcggt taccagtatg tgcacaacgg cggtacggcg tctggcactg
     8821 ttgtaaacag tgacggctgg cagattatca aggaaggtgg tctggcggat ttcaccaccg
     8881 ttaaccagaa aggcaaactg caggtgaacg ccggtggtac cgccacacat gtcaccctga
     8941 aacagggtgg agcactggtt accagtactg cggcaacggt tcttggcagc aaccgtctgg
     9001 gcaatttcac ggtggaaaac ggtaaggctg acggagttgt tctggagtcc ggcggtcgcc
     9061 tggatgtact ggaaggccat tcagcgcaga aaacacgggt ggatgacggc ggaaccctgg
     9121 cagtgtctgc cggtggtaag gcaacaggtg tcaccatgac atccggtggt gccctgattg
     9181 cagacagtgg tgccactgtt gaggggacca atgccagcgg taagttcagt attgatggca
     9241 catccggtca ggccagcgga ctgctgctgg aaaatggcgg cagctttacg gttaatgccg
     9301 gaggactggc cagcaacacc actgtcggac atcgtggaac actgacgctg gctgccgggg
     9361 gaagtctgag tggcagaaca cagctcagta aaggtgccag catggtactg aatggcgatg
     9421 tggtcagtac cggcgatatt gttaacgcag gggagattcg ctttgataat cagacgacac
     9481 cggatgccgc gctgagccgt gctgttgcaa aaggcgactc cccggtaacg ttccataaac
     9541 tgaccaccag taacctcacc ggtcagggtg gcaccatcaa tatgcgtgtt cgccttgatg
     9601 gcagcaatac ctctgaccag ctggtgatta atggtggtca ggcaaccggc aaaacctggc
     9661 ttgcgtttac aaatgtcgga aacagtaacc tcggggtggc aacctccgga cagggtatcc
     9721 gggttgtgga tgcacagaat ggcgccacca cagaagaagg tgcgtttgcc ctgagtcgcc
     9781 cgcttcaggc cggcgccttt aactacaccc tgaaccgtga cagcgatgaa gactggtacc
     9841 tgcgcagtga aaatgcttat cgtgctgaag tccccctgta tacatccatg ctgacacagg
     9901 caatggacta tgaccggatt ctggcaggct cccgcagcca tcagaccggt gtaaacggtg
     9961 aaaataacag cgtccgtctc agcattcagg gcggtcatct cggtcacgat aacaacggcg
    10021 gtattgcccg tggagccacg ccggaaagca gcggcagcta tggcttcgtc cgtctggagg
    10081 gtgacctgct gagaacagag gttgccggta tgtctctgac gacaggggtg tatggtgccg
    10141 caggccattc ttccgttgat gttaaggatg atgacggttc ccgcgccggc acggtccggg
    10201 atgatgccgg cagcctgggc ggatacctga atctgacaca cacgtcctcc ggcctgtggg
    10261 ctgacattgt ggcacaggga acccgccaca gcatgaaagc gtcatcggac aataacgact
    10321 tccgtgcccg gggctggggc tggctgggct cactggaaac cggtctgccc ttcagtatca
    10381 ctgacaacct gatgctggag ccacaactgc actatacctg gcagggactt accctggatg
    10441 acggccagga taacgccggt tatgtgaagt tcgggcatgg cagtgcacaa catgtgcgtg
    10501 ccggtttccg tctgggcagc cacaacgata tgagctttgg tgaaggcacc tcatcccgtg
    10561 acactctgcg cgacagtaca aaacaccgtg tgagtgaact gccggtgaac tggtgggtac
    10621 agccttctgt tatccgcact ttcagc
//
LOCUS       DAEBXN010000131        10464 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.131, whole genome shotgun sequence.
ACCESSION   DAEBXN010000131 DAEBXN010000000
VERSION     DAEBXN010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10464
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.131"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            3..203
                     /gene="cspG"
                     /locus_tag="KJL99_003585"
     CDS             3..203
                     /gene="cspG"
                     /locus_tag="KJL99_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001990088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HBD4438175.1"
                     /translation="MTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRTLNEN
                     QKVEFSIEQGQRGPAAANVVTL"
     gene            214..402
                     /locus_tag="KJL99_003586"
     CDS             214..402
                     /locus_tag="KJL99_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBD4438176.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            377..607
                     /gene="ymcE"
                     /locus_tag="KJL99_003587"
     CDS             377..607
                     /gene="ymcE"
                     /locus_tag="KJL99_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HBD4438177.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGKM
                     AYNISYLTRVPGTNTLLACCCLSRPDEVNSEY"
     gene            597..770
                     /locus_tag="KJL99_003588"
     CDS             597..770
                     /locus_tag="KJL99_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HBD4438178.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFIAREKM
                     TGLESYDVKIKIM"
     gene            complement(819..1892)
                     /locus_tag="KJL99_003589"
     CDS             complement(819..1892)
                     /locus_tag="KJL99_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBD4438179.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQFLLLAMNI
                     YIAITFWYWVRYYETAGSTTFVARPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSLFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKKCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(1994..4705)
                     /gene="torS"
                     /locus_tag="KJL99_003590"
                     /pseudo
     CDS             complement(1994..4705)
                     /gene="torS"
                     /locus_tag="KJL99_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
     gene            4788..5816
                     /gene="torT"
                     /locus_tag="KJL99_003591"
     CDS             4788..5816
                     /gene="torT"
                     /locus_tag="KJL99_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HBD4438180.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHFTVQASMPLKAKRV
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(5789..6481)
                     /gene="torR"
                     /locus_tag="KJL99_003592"
     CDS             complement(5789..6481)
                     /gene="torR"
                     /locus_tag="KJL99_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HBD4438181.1"
                     /translation="MPHHIVIVEDKPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDYCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            6568..7740
                     /gene="torC"
                     /locus_tag="KJL99_003593"
     CDS             6568..7740
                     /gene="torC"
                     /locus_tag="KJL99_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HBD4438182.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDITGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNECHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            7740..10280
                     /gene="torA"
                     /locus_tag="KJL99_003594"
                     /pseudo
     CDS             7740..10280
                     /gene="torA"
                     /locus_tag="KJL99_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            10277..>10464
                     /gene="torD"
                     /locus_tag="KJL99_003595"
     CDS             10277..>10464
                     /gene="torD"
                     /locus_tag="KJL99_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="TorD; involved in the biogenesis of torA; acts on
                     torA before the insertion of the molybdenum cofactor and,
                     as a result, probably favors a conformation of the
                     apoenzyme that is competent for acquiring the cofactor;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HBD4438183.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNDLENSIAT"
BASE COUNT         2525 a         2684 c         2788 g         2467 t
ORIGIN      
        1 aaatgactgg tttagtaaaa tggtttaacg cagataaagg ttttggcttt atcactcctg
       61 atgatggcag caaagacgtt ttcgtccatt tcaccgccat ccagagcaat gaattccgca
      121 cgctgaacga aaatcagaaa gttgaatttt ctattgagca ggggcaacgt ggccccgcgg
      181 cagcgaacgt tgttacgctc taaggttgcc gttattactc aacatctcca tttccgctgt
      241 ccatgttgtc atggttcaca gtaccgtaca tcggcattcg atgtgacgga gcgaaaccct
      301 ttgggcgcta agtgtatttt ttgtaaatcg actatgatca cctttgataa cgtcgcgctg
      361 caaatacgca ctgaccatgc gccgctggat ttcacaaaat aatatcaggc tccctcgtgg
      421 agcctttttt atatctgcct tatttttctt caacgctgta tgtatagtaa gcgataacct
      481 gttgattatt gaatctttcg ggaagatggc ttataacatt tcttacctga ccagggtacc
      541 gggaaccaac accttactgg cgtgttgctg tctttcaaga ccagacgagg ttaacagtga
      601 atattgaaga gttaaaaaaa caagccgaaa cggaaatcgc cgactttatc gcgcaaaaaa
      661 tcgccgagct gaacaagaat acagggaaag aagtctctga aattcgcttc atcgcacgag
      721 aaaaaatgac cgggcttgaa agttatgatg tcaaaatcaa aataatgtga ttttgtgaac
      781 atcaccccgt gcgaggtgat gttccgcttg ttgctaattt agtgaccaat cattggcgct
      841 tgtggaatta agcgtcggta caattcctcc ggcaccgggc tttgccatac tcccgcatac
      901 attgcgtaac caatcaccgc aaacataatc cccagaacca gtagcgtcat taaccagcca
      961 gacaacgcaa aggctttttt atttgctgca ggtttttgca gtgaaaaggt caatgttgag
     1021 gctaccggac atgactctac gcaagtcata cagccggtac attccactgt tcgtacctga
     1081 attaatttat ctaccgggat ccgtgatggg cacttttttg cgcatttgcc acaatcgata
     1141 caactttcgg cattgcgacg aatcttaaac ggcgacaata gcgaaaccac gcccatcagc
     1201 gcgccatatg ggcaaagata acgacaccag gcatggcgaa taaacaggct ggcaatcagc
     1261 aaaacggtca cgctgattaa tgtcgcggtc cccatatgac gaaagaaatc gagcatttta
     1321 acgtccatca ccacgctata gggcgacaac ataaaatagt gaatcgcctg agcgggcatc
     1381 aataacgcga tatagagaaa taaactcaac agcaaatact tcacgccgcg cagaggaata
     1441 tccagccagc ggggaaggac acattgccga ccaaacagtt tgttaccgag atcgccgatt
     1501 aattcagaaa gcgtaccaac cgggcataac catgagcaaa aggccttttt gagtaataga
     1561 ctgatgacga taaaagcgac caataacagc atcgcggcgg cgtggacgga cggtaactga
     1621 cctgttgcaa ggctatattt cagattcatc agaccggcaa tcggtagcca gccttcgata
     1681 cctcccggtc tggcgacaaa tgtcgtacta cctgccgttt cgtaatagcg cacccaatac
     1741 cagaacgtga tggcaatata aatattcatt gccaatagta agaattgcgt cgctttacgc
     1801 caggtcgtgg cattgcgcca gtcattccac ggtaatttgc cgcccgtcgt gcctggccgc
     1861 cgctgccagc gggttctttt tttctctgcc atgattttgc cagtccgtta agttgcatac
     1921 caaatgccac tattctagtt attcttaact ggctgatatt gattcaaatc gcgttcaggt
     1981 ctttcttatt caatcatgct tccagagcgg caacactgcg tgtaatttct tcgtgtggaa
     2041 ggggggcaga taatggctgc tgctccagtt gtgcgcatag ctgactggcg ctacgcattc
     2101 ccagactcga gcaactgctt tttagctgat gcgcggcacg ctttattttc tcgctgtcct
     2161 ggctggcgcg ggcaatgtcg atttcatcga gaagcggcag ggcatgttgt ttaaataatg
     2221 ccagccattc gtggatcttc tccgtcccca ttaactgagc gtcttcattg agttgcgaga
     2281 catccagtgg tagatcgtta ttggcttgca gttggagata gtgcgccagt aactgaccga
     2341 gcacttcacg cggcaccggt ttagggataa tcccgcgaaa tagtgaactg gtacgctggc
     2401 gcagcgtttc gtcaatgaca tgggcgctaa agccaatcaa aaccagcgac ggatattgct
     2461 gtgccagttg ccgggcaagg gtaatgccgt cgatatccgg cagatcaaaa tccaccagtg
     2521 cggcagcaaa cggttcgcta ttttgcagtg tctctaaagc ctgcgcggca ttgccaatag
     2581 caacaacctg cgcaccactg gtgttcagca tctcaacggt aattcgctgg gttagcgggt
     2641 tatcttcaat taacagcaaa cgtaaaccgt caagacgcac cgcctgattg gctgtttttg
     2701 gcacgggtgc agtggcaaca cgtaacggca agcgtaaaca aaaacagctt ccaacctccg
     2761 gcgtgctggt ggcgctcagt tcgccgccca tcgcctgggc cagacggcta ctgatagtca
     2821 gccccagccc ggtgccgccg cgtttgccgc ttacctgcac aaatggctgg aagatttctg
     2881 ccagtttcgc gggatcaata ccgcagccgc tgtcttccac ttcgaccagc cattgctcgc
     2941 catcagtgcg actacgcagg ataatgtgcc cttcgtcagt aaaacgcagg gcattgctca
     3001 ataggttggt aatgacctga cgaatacgtc gtggatcgcc cattaacgcg ctcggtacat
     3061 catcggcaat tgccgttgcc aggcgaatcg ggcgaccttt cacccgtccg ctcattaatt
     3121 gcagggtact ttccagcagc gggcgcggtt caaagggctc atcgctgact gaaacatttt
     3181 tgccacctgc ttcgatagcc gaataatcga gaatatcgtt gaggatggtc agcaacgatt
     3241 cgccagagtc ggtaatcgcc tgcaaatcat cacgctgggc gttaagtgcg gggttatctg
     3301 ccagcagttg agcagtgccg agaataccgt acagcggtgt gcggatctca tggctcatcg
     3361 ccgtcagaaa cgccgatttt gcctggctgg ctttttctgc ttccgcccgt gcctgtcggt
     3421 gttctatcac cagttcctgc aattcagctg tacgcgcttt gacctgcgcc gccagctgtt
     3481 cgcggtggcg attcagtgca tgaacactgc tgcgaaacgc atccatcagc cgcccgatgg
     3541 tatccagctc ccgtacgcca gcggtttccg ggaaagggga gtcaatatca ccgtccagca
     3601 gccgttgcag cgcctgcgtt tgttcggcaa gtggacgcgt gaatgagcga taaaccacgc
     3661 gccagaggat cataatcagt gcgcaaagtg aaaccatccc cagcaataac aggctgtatt
     3721 gcccgcgtgc actggctttt tccagatgtg ccagtccatg ctgattgtgc agctcaatgg
     3781 tgtcgaccag ctgactgact tcgctactaa actgcgcgaa ctgggcgata ttattttggg
     3841 cgagagtttg caggtgattg ctgatttcac tgtcctgctg gaacagcgcc aacagatcgc
     3901 tatattggct aacggtagtt aacgttgttg cgacctgcgc acgaactccc ggatcttcaa
     3961 tgcgtatttg ccgacgttgc agaattttca ccgcattatt gagctgcttt tccagcgttg
     4021 gtgcattttt ctggatctgc tccagcccca gattcatcac catttgctgc acccgcagag
     4081 cgctaaggcg cagttcattc atctggttaa catactcaag atcgatatca atcagccgat
     4141 cgagtgcact ttcagcagcc tgacgctgat cttgttcgat caaatcgtaa atcccggcct
     4201 gggtcgctcc agcggaagtt gtcgcattat tcgcctgacc ttgctccagg cgtgcgatct
     4261 catcggcggc agctatctgc tgactgagtt gccgttgttg ctggcgtagt tgcagacgcc
     4321 gccccaccag ttccccttgc tgacgtaatg aacgggagat ctcctgctcc tgttgttcaa
     4381 tagcggtggt atcaaaacct tgttcccgta acgcttgcag caacgcatta atcttcaggc
     4441 tttatgcggt gagcattcgc ccctgcgcct gccacatctt ttcgttatcg gcactggtca
     4501 ggttctgcgc ggcgaaaagt tcccaggcgc tggcttcgct caactggcgc gccatattca
     4561 tggtaggaat caatgcctga gtattgtctt tttccacctg gctgataaag cgcaggttgt
     4621 accatcccac cagggtactg gtcagggtta acagcgccat cagggcaaag cccatccaga
     4681 gtcttcgggt cagggttaaa ttcacggtcg gtgcacttaa ggtgaaaaag gttgagtcgc
     4741 aaagtggaat gcatctagca taaagcctta ttattgatga ggctatcatg cgcgtactgc
     4801 tatttttact tctttccctt ttcatgttgt cggcattttc ggctgataac ctgttgcgct
     4861 ggcatgatgc gcagcatttc acggtgcaag cctctatgcc gcttaaagcc aaacgcgtat
     4921 ggaaactgtg cgcgctttat cccagcctga aagattcata ctggttatcg ttgaactatg
     4981 gtatgcagga ggctgctcgc cgctacggtg tggatttaaa agtgctggag gcaggcggct
     5041 acagccagtt ggctacccag caagcacaaa tcgaccagtg taaacagtgg ggcgcagagg
     5101 ccattttgct cggtagtagc acgacgtcat ttcccgacct gcaaaagcag gtagcaaatc
     5161 tgccggtgat cgaactggta aatgctcttg atgatcccca ggtgaaaagc cgcgttggtg
     5221 taccctggtt tcagatgggc tatcaacctg ggcgatattt ggtgcaatgg gcgcacggta
     5281 aaccactgaa cgtgctgctg atgcccggac ccgataacgc cgggggcagt aaggagatgg
     5341 tcgagggttt tcgcgcagcc attgccggaa gcccggtgcg tattgttgat attgcgcttg
     5401 gtgataacga tattgaaatc cagcgcaacc tgttgcagga gatgctggaa cgccacccag
     5461 aaatcgacgt cgttgccgga acggccattg cggcagaggc ggcaatgggt gaagggcgta
     5521 acctgaaaac gccgcttacc gtggtgtcgt tttatctttc acatcaggtg tatcgcgggc
     5581 tgaagcgggg aagagtgatt atggctgcca gcgatcaaat ggtctggcag ggggaactgg
     5641 cagttgagca ggccatcagg caattacagg ggcaatcggt gtctgataat gtcagcccac
     5701 cgattttagt tctgacgccg aaaaatgccg accgagaaca tatccgccgc tcgctgtcac
     5761 cggggggatt tcgtccggtc tatttttatc agcacacatc agcggctaag aaataacctt
     5821 caccatgttg cgtcaccagt aaatccgcgc tgagtttatg acgtaaacga cgaattaaca
     5881 catcgacggt gcgcaggtca gggttttcca cccgacgcgc agaaagcata cgcagcagac
     5941 gctcacggct gagaatttcg cccggattag tcacaaatgc caccaacatt tcatactctg
     6001 cgcgggtcag tttaatcggc tcgccatccc gctccagcgt atggcgcgac acattcaggc
     6061 aataacccgc aaagcgatag cagtagtcct gagtgtgcgg ttgagcttgt cgcgcgaggt
     6121 cgattcgcca gagcagattt ttcacccgta ctaccagttc gcgcagttcc agcggtttgg
     6181 tgacgtaatc gtctgcgccc atttccagcc caacaatacg gtcaatccga tcgctgcgtc
     6241 cggtaaccag aataatcccc accgttgagc gttctcgcag ggcgcgggtt aacatcaggc
     6301 cattttcatc gggtaagttg atatccagca gaattaaatc taccggctga ttctgcataa
     6361 tttcccgtag cccggcaccg ctcgccgtaa cggaaacggt atacccctcc tgagtgaagt
     6421 aggattgtaa tcgcgcctgg gtaaccggct tatcttcaac aataacaatg tgatgtggca
     6481 tcagagggtt ttactcattc tgttcatatc tgttcatatt tgctcattaa gatcgcttca
     6541 ctaaaccata attctacagg ggttattatg cggaaactct ggaacgcgct acgccgaccc
     6601 agtgctcgtt ggtcggtact ggcgctggtc gcaattggga ttgtgattgg cattgcgctg
     6661 attgtattgc cacacgttgg gatcaaagtc accagcacaa ccgaattttg tgtcagttgc
     6721 cacagtatgc aaccggtgta tgaagaatat aaacagtcgg tgcatttcca gaacgcctcc
     6781 ggcgtgcgag ctgaatgcca tgactgtcat atcccgccgg atattacagg catggtgaag
     6841 cgcaaactgg aagcgagcaa cgacatttac cagaccttta ttgcccactc cattgataca
     6901 cctgaaaaat tcgaagccaa acgcgcggaa cttgccgagc gtgaatgggc gcgaatgaaa
     6961 gaaaacaact cggcaacctg tcgctcctgc cataactacg atgcgatgga tcatgccagg
     7021 cagcatcctg aagcggcacg tcagatgaag gtggcagcga aagataatca atcctgcatc
     7081 gactgtcata aaggtattgc ccaccagtta ccggatatga gtagcggctt ccgtaagcag
     7141 ttcgatgagc tgcgcgccag tgctaatgac agtggtgaca cgctgtactc tattgatatt
     7201 aagccaattt atgcggcgaa aggcgataaa gaagcctctg gttctctgct gcctgcttcg
     7261 gaagtgaaag tccttaaacg tgacggcgac tggctgcaaa ttgaaattac cggctggacg
     7321 gaaagcgccg gacgtcagcg tgtactcacc caattcccag gtaaacgcat ctttgttgcc
     7381 tcgattcgtg gtgatgtgca gcagcaggtg aaaacgctgg agaaaaccac cgttgccgac
     7441 accaataccg agtggagcaa gttgcaggcc accgcgtgga tgaagaaagg cgacatggtg
     7501 aacgatatca aaccgatctg ggcctatgcg gattcgttgt acaacggcac ctgtaacgag
     7561 tgccacggcg caccggaaat cgcccacttt gacgctaacg gttggatcgg cacgctcaac
     7621 ggcatgattg gctttaccag tctcgataaa cgtgaagaac gcactttgtt gaaatatctg
     7681 caaatgaatg cgtctgacac cgcaggtaag gctcacggcg ataagaagga agaaaaataa
     7741 tgaacaataa cgatctcttt caggcatcac gtcggcgttt tctggcacaa ctcggcggct
     7801 taaccgtcgc cgggatgctg gggccgtcat tgttaacgcc gcgccgtgca actgcggcgc
     7861 aagcggcgac tgaggctgtc atctcgaaag agggcattct taccgggtcg cactgggggg
     7921 ctatccgcgc gatggtgaag gatggtcgct ttgtggcggc aaaaccgttc gaactggata
     7981 aatatccgtc gaaaatgatc gccggattgc cggatcacgt acacaacgcg gcacgtattc
     8041 gttatccgat ggtacgcgtg gactggctgc gtaagcgcca tctgagcgac acctcccagc
     8101 gcggtgataa ccgttttgtg cgcgtgagct gggatgaagc cctcgacatg ttctatgaag
     8161 aactggaacg cgtacagaaa actcacgggc cgagtgcctt gctgaccgcc agtggttggc
     8221 aatcgacggg gatgttccat aacgcttcgg ggatgctggc gaaagctatt gccttgcatg
     8281 gtaatagcgt tggtacgggc ggagattact ctaccggtgc tgcgcaggtg atcctgccgc
     8341 gcgtagtcgg ttcaatggaa gtgtatgaac agcaaacctc ctggccgctg gtattgcaga
     8401 acagcaaaac cattgtgctg tggggctccg atttgctgaa aaaccagcaa gcgaactggt
     8461 ggtgcccgga tcacgatgtt tatgaatatt acgagcagct aaaagcgaaa gtcgccgccg
     8521 gtgaagttga ggtcatcagc atcgatccgg ttgtcacatc cacccatgag tatctggggc
     8581 gcgagcatgt gaagcacatt gcggttaacc cgcaaactga cgtgccgctg caactggcgc
     8641 tggcacatac gctgtacagt gaaaacctgt acgacaaaaa cttcctcgct aactactgtg
     8701 tgggttttga gcagttcctg ccgtatctgc tgggtgagaa agacggtcag ccgaaagatg
     8761 ccgcatgggc tgaaaaactg accggcattg atgccgaaac cattcgtggg ctggcgcggc
     8821 agatggcggc gaacagaacg caaattattg ctggctggtg cgtgcagcgt atgcagcacg
     8881 gtgaacagtg ggcgtggatg attgtggttc tggcggcgat gctggggcaa attggcctgc
     8941 caggtggtgg ttttggctgg cactacaacg gcgcaggcac gccggggcgt aaaggcgtta
     9001 ttctgagtgg tttctccggc tctacgtcga ttccgcctgt tcacgacaac agtgactaca
     9061 aaggctacag cagcactatt ccgattgccc gttttatcga tgcgatcctc gaaccgggga
     9121 aagtgatcaa ctggaacggt aaatcggtaa aactgccgcc gctgaaaatg tgtatttttg
     9181 ccggaactaa cccattccat cgccatcagc agatcaaccg cattattgaa ggctggcgca
     9241 agctggaaac ggttatcgcc atagataacc agtggacctc aacctgccgc tttgccgata
     9301 tcgtgctgcc tgcgaccacg cagtttgagc gtaacgatct cgaccagtac ggcaaccact
     9361 ccaaccgtgg cattatcgcc atgaaacagg tggtgccgcc gtagttcgag gcgcgcaacg
     9421 actttgatat tttccgcgag ctgtgccgtc gctttaatcg tgaagaagcc tttaccgaag
     9481 ggctggacga aatgggctgg ctgaaacgca tctggcagga aggtgtacaa caaggcaaag
     9541 gacgcggcgt tcatctgcca gcgtttgatg acttctggaa taacaaagag tacgtcgagt
     9601 ttgaccatcc gcagatgttt gttcgccacc aggcattccg cgaagatccg gatctcgaac
     9661 cgctgggcac gccgagtggc ctgattgaga tctactcgaa aaccatcgcc gatatgaact
     9721 acgacgattg tcaggggcat ccgatgtggt ttgagaaaat cgaacgctcc cacggtgggc
     9781 ccggctcgca gaagtatccg ttgcatctgc aatctgtgca tccggatttc cgacttcact
     9841 cgcagctatg tgagtcggaa acgctgcgtc agcaatatac ggtagcgggt aaagagccag
     9901 tgttcattaa cccgcaggat gccagcgcgc gcggtattcg taacggtgat gtggtacgcg
     9961 tctttaacgc tcgcggtcag gtgttggcag gagcagtagt ttctgaccgc tatgcacccg
    10021 gcgtggcgcg aattcacgaa ggggcatggt acgatccaga taaaggcggc gagcctggtg
    10081 cgctgtgcaa atacggtaac cccaacgtgc tgaccatcga catcgggact tcgcagctgg
    10141 cgcaggcgac cagtgcgcac actacgctgg tggaaattga gaagtacaat ggaacagtgg
    10201 agcaggtgac ggcgtttaac ggccccgtgg agatggtggc gcagtgcgaa tatgttcccg
    10261 cgtcgcaggt gaaatcatga ccacgctgac agcacaacag attgcctgtg tttacgcctg
    10321 gctggcgcag ttgttctccc gtgaactgga cgatgaacaa ctgacgcaaa tcgccagtgc
    10381 gcagatggct gaatggtttt cgttgctgaa aagcgaaccg ccgctcactg cggcggtgaa
    10441 tgatctggaa aacagtattg ccac
//
LOCUS       DAEBXN010000132        10446 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.132, whole genome shotgun sequence.
ACCESSION   DAEBXN010000132 DAEBXN010000000
VERSION     DAEBXN010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10446
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.132"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            597..794
                     /locus_tag="KJL99_003596"
     CDS             597..794
                     /locus_tag="KJL99_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438184.1"
                     /translation="MEITPQQLVEFTIPDGRGQQARTLPDMLSSDAGPLSYPLIYSSK
                     VMIISLFDSTVMPDKYRHNGK"
     gene            complement(744..1937)
                     /locus_tag="KJL99_003597"
     CDS             complement(744..1937)
                     /locus_tag="KJL99_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD4438185.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2073..3797
                     /gene="iucA"
                     /locus_tag="KJL99_003598"
     CDS             2073..3797
                     /gene="iucA"
                     /locus_tag="KJL99_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HBD4438186.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3798..4745
                     /gene="iucB"
                     /locus_tag="KJL99_003599"
     CDS             3798..4745
                     /gene="iucB"
                     /locus_tag="KJL99_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HBD4438187.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4745..6487
                     /gene="iucC"
                     /locus_tag="KJL99_003600"
     CDS             4745..6487
                     /gene="iucC"
                     /locus_tag="KJL99_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HBD4438188.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6484..7821
                     /gene="iucD"
                     /locus_tag="KJL99_003601"
     CDS             6484..7821
                     /gene="iucD"
                     /locus_tag="KJL99_003601"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HBD4438189.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFAHNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            7827..10022
                     /gene="iutA"
                     /locus_tag="KJL99_003602"
     CDS             7827..10022
                     /gene="iutA"
                     /locus_tag="KJL99_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HBD4438190.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IATGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFIGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
BASE COUNT         2560 a         2656 c         2749 g         2481 t
ORIGIN      
        1 ggagtgaaac aaaggcagct tatcgttgtt tttcaaacga taaagtctct gctaataaaa
       61 tcatgactcc tcacaaagaa aatatcactg aaagagctca gcagtttaaa agagttttag
      121 ttttacagga tacaacagaa cttaattatt ctggtcagaa agaaaaacaa ggagttggtc
      181 ccaaaaaaca taaagatgaa agaaacttat ttcttcatcc tcaactcgtc atcagtgagt
      241 caggtgtttg cctcggtgtt tatgatgact atcagtggtt cagggatgaa ttaaaaacca
      301 gtaaaaatac ccgacaagaa atctgcaatg atttacttca taaaaaacat gtcagtgaaa
      361 aagaaacctg gcgttgggtt gaaggatata ataaagcaac agaactggct cgccaatgcc
      421 ctgataccca tgtattgtca atttctgatc gtgaaggcga tttttatgac ctgtttgaac
      481 gcgcagcgca gactcccgga ataaaagctg actggcttgt cagaatgaaa tttaataaca
      541 gagcaacgct gaatattaat ggtaaacggg atcataggtt gttgcacgaa agaatcatgg
      601 agattacacc gcagcaactg gttgaattca ctatacctga tgggcggggg cagcaagcca
      661 gaacactgcc tgacatgctt tcatcagacg caggtcctct ctcatatcct ctgatttact
      721 ccagtaaggt gatgatcata tctttatttg atagtacggt aatgccagat aaatacagac
      781 acaatggtaa ataatatagt tgcaaataat gctgccataa tcagcgcgat tgcgacacct
      841 gttttcgata ccagcggcac cagcaaagcg cacagtccat agccaagtgt gtgactggta
      901 gcgagccagc ccgcgcctgc gccatcagcc agcttatcat tcagtagaag ttgataagcg
      961 ggtgttgcca gagctgctcc aaatgacagc actgcacagc ccgggtaaaa caaccatagc
     1021 tgttcggaga gcatgatagc cagaccacca ctcatcaaca ctccggcact caggagcagg
     1081 gccaccggag tcaggcgctg cggacggagt accccgaact gcgcgataag cgcagctact
     1141 gcggacagac ccaacagcca ggccacctgt tggctaatgg cggtggtatc cgtagcgaac
     1201 tggcgagtaa gggcaggcga aagtccaagc tgcatcatgc tcaccgccgc agccagtaat
     1261 attgcgcaaa gcagataagg cagacaatcc cgcttcagac tgacgctctt gcactccggt
     1321 gtgggctgtg gtggtgtgcc gggcaaccgc agcagcatca gcagcgccag cactggggct
     1381 gccatcagca gccccagtgg cgccagagga tgaatggcca gcattgctgc cgcgcacagc
     1441 ggcccgaata gcctgccgca actcaggccg gagctgatgg ttgccagagc ggccatgcga
     1501 ttcccttctc cagctctctg caatgcccag acctgacagg ctggcaccat tgccgacacg
     1561 gtcagcccgt aggcgatccg ggcgatgatt aatattccca gtcccaccgc tgttgttatg
     1621 gcgctggtag ccatcagcac gcttcccagc cctagcaggg taaagcttat gccatatcct
     1681 cccagagccc acaacaccac aggtttactg ccagtacggg aaatttgctt tccccaccac
     1741 ggggacgatg gcagaaacag cattgagcca agcatcagca gggcagccca tacagacaga
     1801 ctcagatttg tctgtatgac cagaacaggg agcacaacca gcagaccgtt ctgtccgata
     1861 ccgagaagcc cggcactgaa cgcaagtggc cagcaggaca gtggtttttg gggcgtatct
     1921 tcgatccctg gtgacattat aatgtttcaa ctccatgtat taattgtgtt tatttgtaaa
     1981 attaatttat ctgacaataa catttattgt tgataatgag aatcattatt gacataattg
     2041 ttattatttt actgtgtggg agctgtttga ctatgaccct gccctctgaa aaaccagcca
     2101 cagatgtggc tgcgcagtgc ttcctgaatg cactgattcg tgaaaccaca gactggaaac
     2161 tgacagaata cccgccagac gaattgctca tcccgctgga tgagcagaaa tcgctccatt
     2221 tcagagtggc ttatttctcc ccaacccagc atcaccgctt tgcatttcct gcacgtctgg
     2281 tcacggcatc aggcagttat cctgtcgact ttaccactct ctcccggctt attattgata
     2341 agctacgcca tcaactgttt ctgcccgttc ccctctgcga aactttccac cagcgcgtgc
     2401 tggaaagcca tgtccatacg caacaggcaa ttgatgcccg tcatgactgg gccgctctgc
     2461 gtgaaaaagc gttgaatttt ggcgaggctg agcaggcgct gctgacagga cacgctttcc
     2521 accctgcgcc taagtctcat gaaccgttta accggcggga ggctgaacgc tacctgcctg
     2581 atatggcacc ccacttccca ctgcggtggt tttcggtgga taaaacgcaa atcgccggtg
     2641 aaagtttgca tctcaacctt caacagcggc tgacgcgatt tgccgcagag aatgcgcctc
     2701 agttactcaa cgaattaagt gacaaccaat ggctgttccc gttgcacccg tggcaggggg
     2761 aatatctttt gcagcagggg tggtgccagg cacttgttgc taaagggctg attaaagact
     2821 taggtgaggc cggcacgtcg tggctgccga ccacctcttc ccgttccctc tactgtgcca
     2881 ccagccgcga tatgatcaag ttctccctga gcgtacggct gaccaactcc atccgtactc
     2941 tgtccgtgaa agaagtgaag cgtgggatgc gcctggcacg cctggctcaa accgacggct
     3001 ggcagatgct acaggtccgc ttcccgactt tccgggtaat gcaggaggat ggctgggccg
     3061 ggctgctcga tcttaacggc aacatcatgc aggaaagtct gtttgccctg cgtgaaaatc
     3121 tgctggtgga tcagccgaaa agccagacta acgtactggt ctccctgact caggccgcac
     3181 ctgatggcgg tgattcgctg ctggtttcgg cggtaaaacg cctgagcgat cgcctcggta
     3241 tcactgtgca acaggccgcc catgcatggg ttgatgcata ctgtcagcaa gtgctaaagc
     3301 cgctgtttac ggctgaagcg gattacggcc tggtgctgct ggcgcatcag caaaatattc
     3361 ttgtccagat gcttggggat ctgccggtcg gatttattta ccgtgactgt cagggcagcg
     3421 cttttatgcc tcacgcgaca gactggctcg attctattgg cgaggcgcag gcggaaaata
     3481 ttttcaccca tgagcagttg ctgcgctatt tcccttatta cctgctggtt aactccactt
     3541 ttgctgtgac cgctgcgctg ggggctgccg ggctggacag cgaatcgaat ctgatggctc
     3601 gtgtacgagc atcgctggct gaagtgcgtg atcaggtgac tcataaaacc tgtctcaact
     3661 acgtgctgga aagtccgtac tggaacgtaa aaggtaactt tttctgttat ctgaacgatc
     3721 ataacgagaa caccattgtt gacccttcgg tgatctattt cgatttcgct aacccgctgc
     3781 aggctcagga ggtctgaatg tctgaggcaa acattattca cagcagatat ggactgcgct
     3841 gtgaaaaact cgacaagccc ctgaatcttg gctggggact ggacaatagc gcggtgttgc
     3901 actgtcccgg ggagctgccg acagggtggc tgtgcgatgc gctggatcag atatttatcg
     3961 ccgcaccaca actgtcagca gtggctctcc cctgggccga atggcgtgag gagccacagg
     4021 cgctgacgct ttttgggcag gtaaaaagcg acattatcca tcgtactgcc ttctggcagt
     4081 taccgttatg gttgagttca ccggcaaacc gggcctccgg tgaaatggtt tttgatgcag
     4141 agcgtgagat ttatttcccg cagcgcccac cccgtccaca gggtgaggtt taccgccgtt
     4201 acgatccacg tattcgcagg atgctgagtt tccgcattgc cgaccccgtt tctgatgcag
     4261 aacgtttcac ccgctggatg aatgatccgc gcgttgagta tttctgggag caaagtggct
     4321 cactggaggt acagaccgcc tatctggagc gccagctcac cggtaaacat gcattcccgc
     4381 tgattgggtg cttcgacgat cggccattca gctatttcga aatctactgg gcggcggaag
     4441 accgcattgg tcgtcactat tcgtggcagc cctttgaccg tggcctgcat ctgctggttg
     4501 gtgaacagca atggcgcggg gcccactatg tgcaaagctg gctgcgcggg ctgacacatt
     4561 acctgctgct ggatgagccc cgtacgcagc gcacagtact ggagccacgc accgataacc
     4621 agcgcctgtt ccgccatctt gagcctgcgg gataccggac aattaaagag ttcgacttcc
     4681 cacataagcg ctcgcgcatg gtgatggcgg atcgtcatca cttcttcacg gaggtcggtc
     4741 tgtaatgaat cacaaggact gggattttgt taaccgtcgg ctggtggcaa aaatgttgtc
     4801 tgagatggag tatgagcagg ttttccacgc ggaatctcag ggcgatgacc actactgcat
     4861 taacctgccg ggagcacaat ggcgcttcat cgctgaacgt ggtatctggg gctggctgtg
     4921 gattgatgcc caaacgttgc gctgcacgga cgaaccagta ctggcgcaga cgctgctgat
     4981 gcagctaaag ccggtgctgt caatgagtga tgccactgtt gcagagcata tgcaggattt
     5041 gtatgccacg ctactgggcg acctgcaact gctgaaggcc cgtcgcggac tgagtgccag
     5101 tgacctgatt aatcttgatg ccgatcgtct gcaatgcctg ctgagcggtc atcctaaatt
     5161 cgtttttaat aaaggtcgcc gtggctgggg caaagaggcg ctggaacgat atgcgccaga
     5221 gtataccaac accttcagac tgcactggct ggcggtaaaa cgtgaacata tgatctggcg
     5281 ctgcgataac gatctggata ttcagcagtt gttgactgcc gcaatggatc cgcaggagtt
     5341 tacacgcttc agtcaggtct ggcaggaaaa cgggctggat cacaactggc tgccgctgcc
     5401 ggtacatccg tggcagtggc agcaaaaaat cgccacagac ttcatcgctg attttgccga
     5461 aggcaggatg gtgtctctcg gcgagtttgg cgaccagtgg ctcgcccagc agtcgctgcg
     5521 taccctgacc aacgccagcc gccggggagg gctggatatc aagctgccgc tgaccatcta
     5581 caatacctca tgctaccggg ggatccctgg cagatatatc gctgccggac cactggcttc
     5641 acgctggctg caacaggttt ttgcgaccga cgccaccctg gtgcaaagcg gcgcagtgat
     5701 ccttggtgaa ccggctgcag gctatgtgtc ccatgaaggc tatgccgcgc ttgcccgggc
     5761 tccctatcgc taccaggaaa tgcttggtgt tatctggcgg gagaatccgt gccgctggct
     5821 gaaaccggat gaaagtcctg ttctgatggc aacactgatg gagtgcgacg aaaacaatca
     5881 gccgctggca ggcgcatata tcgaccgctc cggactggac gctgaaacct ggcttacgca
     5941 attgttccgg gtggttgtgg ttccgctgta tcacctgctt tgccgctacg gtgtcgcgct
     6001 tattgcacac ggacaaaata taacgctcgc catgaaagag ggggtaccac agcgtgttct
     6061 gctgaaagac ttccagggcg atatgcggct ggtgaaagaa gcgtttcccg aaatggactc
     6121 tttgcctcag gaggtgcgtg atgtcacatc ccgtctgagt gcggactact taatccatga
     6181 tttgcagacg ggtcacttcg tgacagtact gcgttttatt tcgccactga tggttcgtct
     6241 tggcgtacct gaaaggcgat tttatcaact gctggcagca gtgttgagtg attacatgaa
     6301 caaacatcca caaatggcag agcgttttgc gcttttctca ctcttcaggc cacaaatcat
     6361 tcgcgtggtg ctgaacccgg taaaactgac ctggccggac ctggatggcg gcagtcgcat
     6421 gctgccgaat taccttgaga atctgcaaaa tccgctgtgg ctggtaactc aggaatatga
     6481 atcatgaaaa aaagtgtcga ttttattggt gtcgggacag ggccatttaa tctcagcatt
     6541 gctgcattgt cacatcagat cgaagaactg aactgtctct tctttgacga acatcctcat
     6601 ttttcctggc atccgggtat gctggtaccg gattgtcata tgcagaccgt ctttctgaaa
     6661 gatctggtca gtgcagttgc acctacaaat ccctacagct ttgttaacta tctggtgaag
     6721 cacaaaaagt tctatcgctt ccttacaagc aggctacgta cagtatcccg tgaagagttt
     6781 tctgactatc tccgctgggc tgctgaagat atgaataacc tgtatttcag tcataccgtt
     6841 gaaaatattg atttcgacaa aaaaagtcga ttgtttctgg ttcagaccag tcggggagaa
     6901 tattttgccc acaatatctg ccttggtaca ggaaaacaac cttatttacc accctgtgtg
     6961 aagcatgtga cacaatcctg tttccatgcc agtgaaatga atcttcgtcg gccggacctg
     7021 agtggaaaac ggataaccgt ggttggtgga ggacagagtg gtgcagacct gttccttaat
     7081 gcattacgcg gggaatgggg agaagcggcg gaaataaact gggtctcacg gcgtaataat
     7141 tttaacgcac tggatgaggc tgcttttgct gatgagtatt ttacacctga atatatttca
     7201 ggcttctccg gactgaagga agatattcgc catcagttac tggatgagca gaaaatgaca
     7261 tcggatggca tcactgccga ttctttactg accatttatc gtgagttgta ccaccgtttt
     7321 gaagttctga gaaaaccaag aaatatccgt ctgctaccca gtcgctcggt aacaactctg
     7381 gaaagtagtg gtccgggctg gaggttattg atggaacatc atctggatca gggcagagag
     7441 agcctggaaa gtgatgtggt gattttcgcc acaggttacc gttctgcatt gccacaaata
     7501 cttccctcac tgatgccctt gatcaccatg cacgataaga acacctttaa agtgcgtgat
     7561 gacttcactc tggaatggag tggcccgaaa gaaaataaca tctttgcggt caacgccagc
     7621 atgcaaactc atggcatcgc cgaaccccag ctcagcctga tggcctggcg atctgcacgt
     7681 attcttaatc gcgtactggg gcgtgattta ttcgatctca gtatgccgcc cgcactgatt
     7741 cagtggcgca gcggcagccg gaaaaaacca cagccggagg ctgcttcttt aactcactat
     7801 acagcaaata ttcaggaata acgatgatgc gcaaaaagta tatgccccgg gctcttggtc
     7861 cgctgcttct tgtcgtgctg tcaccagctg tcgcccagca aaacgatgat aatgagatca
     7921 tagtgtctgc cagccgcagc aatcgaactg tagcggagat ggcgcaaacc acctgggtta
     7981 tcgaaaatgc cgaactggag cagcagattc agggcggtaa agagctgaaa gacgcactgg
     8041 ctcagttaat ccccggcctt gatgtcagca gccagagtcg aaccaactac ggtatgaaca
     8101 tgcgtggccg cccgctggtt gtcctgattg acggtgtgcg cctcaactct tcacgttccg
     8161 acagccgaca actggactct gtcgatcctt ttaatatcga ccatattgaa gtgatctccg
     8221 gcgcgacggc cctgtacggt ggcgggagta ccggagggtt gatcaacatc gtgaccaaaa
     8281 aaggtcagcc ggaaaccatg atggagtttg aggctggcac aaaaagtggc tttaacagca
     8341 gtaaagatca cgatgagcgc attgccggtg ctgtctccgg cggaaatgac catatctccg
     8401 gacgtctttc cgtggcatat cagaaatttg gcggctggtt tgacggtaac ggcgatgcca
     8461 ccctgcttga taacacccag accggcctgc agcactccaa tcggctggac atcatgggaa
     8521 ccggtacgct gaacatcgat gaatcccggc agcttcaact gataacgcag tactataaaa
     8581 gtcagggaga cgacaattac gggcttaatc tcgggaaagg cttttccgcc atcagcggga
     8641 gcagcacacc atacgtcagt aaggggctga attctgaccg cattcccggc actgagcggc
     8701 atttgatcag cctgcagtac tctgacagtg atttcctggg acaggaactg gtcggtcagg
     8761 tttactaccg cgatgagtcg ttgcggtact acccgttccc gacggtaaat gcgaataaac
     8821 aggcgacggc tttctcctcg tcacagcagg ataccgacca gtacggcatg aaactgactc
     8881 tgaacagcca actgatggac ggctggcaaa tcacctgggg gctggatgct gagcatgagc
     8941 gctttacctc caaccagatg ttcttcgatc tggctcaggc aagtgcttcc ggagggctga
     9001 acaaccataa gatttacacc accgggcgct atccgtcata tgacatcacc aatctggcgg
     9061 ccttcctgca atccagctat gacattaatg atatttttac cgttagcggt ggcgtacgct
     9121 atcagtatac tgagaacagg gtagatgatt tcatcgacta cacgcagcaa cagaagattg
     9181 ctaccgggaa ggcgatatct gccgacgcca ttcctggtgg ttcggtagat tacgataact
     9241 ttctgttcaa tgctggtctg ctgatgcaca tcaccgaacg tcagcaggca tggttcaatt
     9301 tttcccaggg ggtggcattg ccggatccgg ggaaatatta tggtcgcggc atctatggtg
     9361 cagcagtgaa cggccatctt cccctgacaa agagcgtgaa cgtcagcgac agtaagctgg
     9421 aaggcgtgaa agtcgattct tatgaactgg gctggcgctt tatcggtgac aacctgcgga
     9481 ctcaaatcgc ggcatattac tcgctttcca ataagagcgt ggaaaggaat aaagatctga
     9541 ccatcagtgt gaaggacgac aggcgccgta tttacggcgt ggaaggtgcg gtggactacc
     9601 tgatcccgga tactgactgg agtaccggtg tgaacttcaa tgtgctgaaa accgagtcga
     9661 aagtgaacgg tcaatggcaa aaatatgacg tgaaggaatc aagtccatcg aaagcgacag
     9721 cttacattaa ctgggcgccg gaaccgtgga gtctgcgtgt acagagcacc acttctttcg
     9781 acgtaagcga tgcagagggt aacgatatta atggttacac taccgtcgat tttatcagta
     9841 gttggcagct tccggtggga acactcagct tcagcgttga gaacctcttc gaccgtgact
     9901 ataccactgt ctggggacag cgtgcacctc tgtactacag cccgggttac ggccctgctt
     9961 cactgtacga ctacaaaggc cggggccgaa cctttggtct gaactactca gtgctgttct
    10021 gacggggatg cttttgcaac aagggtatgc tgtaaacaac atggtccttg gtggccatgt
    10081 tgtttacagc cagtataata tacagcctta acaagatatt ccgtctccgg aatgggctgt
    10141 tcaccactga ataacattat gtctgtttta tggatctttg actgaaattt atcgcatatc
    10201 ctcatttcat tgtccgtctg aaaactaacc ttacaagtaa gcgtcgtctc agcaccgtct
    10261 ggcagatcct gaaattcctg agaggatgat ctgcgcccaa tatcctgagc cggaactttg
    10321 ctgcaatgaa ggccaacgaa aaatggggga gtatgttata gagtacttgc tacagggtaa
    10381 aaagaggtac ctgtcgctgg ttctccgttc agtttaaccg ggagattttc tcctgcgcct
    10441 gtcata
//
LOCUS       DAEBXN010000133        10435 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.133, whole genome shotgun sequence.
ACCESSION   DAEBXN010000133 DAEBXN010000000
VERSION     DAEBXN010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10435
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.133"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(<1..272)
                     /locus_tag="KJL99_003603"
     CDS             complement(<1..272)
                     /locus_tag="KJL99_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HBD4438191.1"
                     /translation="MYRAAATHPKIKLLQFKLIPCPGMNKAIPSLIADFTGELHRLIS
                     EHLQCKRRKDKSVEPTPLYYLWSREAVANDATPYLMVLLLNNDVWC"
     gene            complement(366..928)
                     /locus_tag="KJL99_003604"
                     /pseudo
     CDS             complement(366..928)
                     /locus_tag="KJL99_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004714555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
     gene            complement(1132..1338)
                     /locus_tag="KJL99_003605"
     CDS             complement(1132..1338)
                     /locus_tag="KJL99_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HBD4438192.1"
                     /translation="MVYKSDSDELAVLRAENTRLVSLLEAHGIEWRRKPQSPVQCVFV
                     LSTDEKVALFRRLFRGRDDVWALR"
     gene            complement(1445..2008)
                     /locus_tag="KJL99_003606"
     CDS             complement(1445..2008)
                     /locus_tag="KJL99_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HBD4438193.1"
                     /translation="MYNANPNYKMDFMILKDVNEHMDGMFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCKHSMCMEIYRLLSETQTMLAGYYWVMEYTPDKGLHIHFIGYLDGQRHKNS
                     YQISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILGRSRLPEKSNRGRPRHN"
     gene            complement(3383..5218)
                     /locus_tag="KJL99_003607"
     CDS             complement(3383..5218)
                     /locus_tag="KJL99_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438194.1"
                     /translation="MRTFTNMLYDICTVLGLFKEGENPAHKRKSTNFEMHQKFWDQRY
                     NEISRIIDAEGVFSQEQRRIIYARYEHFYYMMNSYPVHSTLKPEFLRSYCLRTFGVIF
                     LVVDMYNTYRPENDSAFYYHIYNFLQKSYCPCLDHADTESDEAAVKRYLREYLAELGF
                     NKEDFHENGKLYALGKYTGTIRKDNGKSKSLMQQYIMAIKNEYKKDYREKKLDKDELE
                     KVLRNIDKFYNAFYSLSVLLDIQRKTKILQSLAYYLRVLVREGLWIHGLYGYAAQYLY
                     DFTSFDTTPYAKKLLEMFYKFQNSAEGTLSRYSVSLDDKSQEYISRLKDLVFNINDKN
                     GCDDAYLKKIISYFGQLQNEAVHVTSCYETLAVYICLIRKNKINDVLQHYDDMERKGL
                     FGELPSGYVRGALSLLRTALEVKVNRKNIKYGSLFYWLDHVKAYQDAFIEKIPLIDPV
                     YKEGEIQYDANNFTLMRVIKMYNCMLEKISTKPYIAPPYITGLLDDVEKVLDKINILI
                     DKEYVYDGKTLAEVIMENKVLSSRERKETMIGLFTGSKKYTLLQCVEKLGVLVHYVKS
                     PVDEIKNVMMLYGDKAENRNRRRMIYDALTIICEDDIRNNPPELS"
     gene            5319..5606
                     /locus_tag="KJL99_003608"
     CDS             5319..5606
                     /locus_tag="KJL99_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000070930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HBD4438195.1"
                     /translation="MSNQTGQLYTPSRILRMAELTRLLGISRSTIYVKINPASKYHDP
                     TFPKPIRLGSASVGWRASAIDEWLMLHTAPAWSEPENKLKELNDDDKTITP"
     gene            5578..7107
                     /locus_tag="KJL99_003609"
     CDS             5578..7107
                     /locus_tag="KJL99_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HBD4438196.1"
                     /translation="MMTTKLSLPELDTTKLPDRCQALLDEMHEETGIRREILLSVMLT
                     VMAASVQDTHEVELSGGQRTSLQIFMCLSSASGSGKTSACAKLIAPVHETEEELHQAY
                     IDDKKNYDRMMEMWTTDKKILERRYKKEIERSPENAAAARAALEKCIANKPEPPVQQV
                     LIVNDATPEGIALKLSQSPSLLLLSDEGGTILDKRFERKSALYNTLWSGQPVNVERAS
                     RPGFRLNDVRLTILILTQPVIFNKFFTLTGDQIRGNGFLARLLFCEPGAIKIMTTEPH
                     TATPVVTQQCASCFRKKSFGSQIRDSLRASRERRAKSKERICMTLSNAASRALERFHK
                     ENMDTVRQNPHMGTFEDIIVRKREQVVRIAALLELEKDPDSTVITLESTNSAIYLIEF
                     YFKHLIYKLESLREISPAEKLDKWLQKRIITTAGYIFQKSYILQYAPYALRKKCVLDE
                     ALDILAEQRKIRIDDNLVVYIGNTITPSELAKKLNIPAFDAGVFICDHQNILKYHRNR
                     L"
     gene            complement(7277..8494)
                     /locus_tag="KJL99_003610"
     CDS             complement(7277..8494)
                     /locus_tag="KJL99_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBD4438197.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVVIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYSDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARL
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQLAKNTKEFA"
     gene            complement(8861..9847)
                     /locus_tag="KJL99_003611"
     CDS             complement(8861..9847)
                     /locus_tag="KJL99_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438198.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRPAFLGRFFLQEIDLFFSSENTLSTFFAKPLWLRLFILRVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRFLWHNDFMMACDMYFRWGHFNK"
     gene            complement(9844..10372)
                     /locus_tag="KJL99_003612"
                     /pseudo
     CDS             complement(9844..10372)
                     /locus_tag="KJL99_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
BASE COUNT         3249 a         2386 c         1965 g         2835 t
ORIGIN      
        1 ttacaccaga catcgttatt aagtagcagc accataagat aaggagtggc atcgttagcc
       61 acagcttccc tactccagag gtaatataaa ggggtgggct cgactgattt atctttacgt
      121 cgcttacact gcaaatgttc agaaattagt ctatgcagct caccagtaaa atcagcaatc
      181 agagaaggaa ttgccttatt cataccaggg caaggtatta atttaaactg taataattta
      241 attttaggat gtgtagcagc agcccgatac agagttgcaa ggacacactt ttgccagagg
      301 gcgttacagg aaagcttaat gtttgattct gtatacataa taaatcacct tatagttaca
      361 ataggtcaaa aaccgctgta gccaaagtta cgctggcctg atgctttagt gtcgagcttc
      421 gtcaaataat ccagacgtac caataagcgc tggtactgct ctgggaaatt aggatcgtta
      481 atatccagga tgtaacgtcc attagcaggg aaatgaacta atccctggtg atcttccagt
      541 tccaacccta aggcactgta ccaggctccg gtaattaacc ctctcagggt gtcagagcgc
      601 tcatagtttc ccagatggta ataggcgtct ttgttaaaaa aaggcagacg tgataatgac
      661 atttaaagga ctgcgtatat tctttcgccc agataataca aaccgacgtt gcgtaggtgc
      721 gtttgccttg ttgtttttta cggtgttgat ctgcattaat tttcgcattc agcgagttac
      781 ggaaacgaga tatctctccg gggatcaggt tagggaaaca ggtaatgata tccccgttgt
      841 caatcacagg agggtaatgg agatcccccc gtattgctgt tattctggaa gtgtttttaa
      901 tattcgtatt gatagttctc agagatatat cacactctag atattttaat taagtatcag
      961 ttcgatatcg gcatattgta cactacctca ggtagatgat gagatcagat acagggatca
     1021 actgacggtg agcgcagtcc ccacatttta tccagggctt actgcatatt cccaacagcc
     1081 attcgttagc caggtagacg aataccctga tttgccgctg gttttgcttt cctatcgaag
     1141 ggcccataca tcatcacgcc cacgaaacaa ccgacgaaat aacgcaactt tctcatccgt
     1201 ggataatacg aaaacgcact gcacagggct ctgcggttta cgtcgccatt caatcccatg
     1261 agcttcaagt aatgagacca gccgggtatt ttctgcccgc agtactgcta gttcatcgct
     1321 gtcagattta taaaccatat gcgttatcac ttgtacctgt ctcccggcgg caccacgact
     1381 gaaattcgtg tgaaggcaaa tccacgcgat aacttacgat agttcggaca taagccccca
     1441 taattcagtt atgtcgtggt cgaccccggt tacttttttc gggaagcctg ctacggccca
     1501 gaataatacc gtgttctttc tgatcctgtt tagccagata gctaagggcg taacgtaagt
     1561 cgtctaccgc agatttatca ctatagtgaa tcacgtgatc gatacgcacc gggtatttgt
     1621 ctttagcccg gcacagatga aaataaccct ccccttccgt gatccgcctc cagatatctc
     1681 ccagttgcct ggatatctga taagagttct tgtgacgttg tccatccagg tagccgataa
     1741 aatgaatatg aagccctttg tctggtgtat attccattac ccagtaatat cccgccaaca
     1801 tcgtctgggt ttcgctaagt aaacggtata tttccataca catactgtgc ttacaggaat
     1861 gcccgaaact gggcgtgtct ttcctgtaag caaaatcaat tctgaaaggt aacagtttag
     1921 aaaaacgttg aaacatacca tccatgtgtt cattcacgtc tttcagaatc atgaaatcca
     1981 ttttgtaatt gggattggca ttatacattt tatagtcctt actttataaa actcacagga
     2041 agagagaatt acgtaaatac tgagagtaga gaaacgccgt accacggcat taataaacga
     2101 agtaaaccag cagcacagac aacctgataa aactgttttg tattaccgca ttactgtgat
     2161 gaggtaatac ttcagaatac agtgagactc tcttaaactg agaaccagta cgaattacaa
     2221 tggaaaaaca cacctcatag aattgagttt caggaagcac cactaaattc tttacaggaa
     2281 gtaatctgac attcacaaaa ctcagtatcg gctagctaca ggcaacatcg ctattaatat
     2341 tgtcgaacca ttaagtaaag ccgagagaga ccacgggtat ttaatcatta gcataaaaga
     2401 ataatggtat acagaacata cccacacacc ataagaagat tgttccatac agaaaagatc
     2461 atatttacct atacctataa actgtaacgg agtcataaca cgctctaata gtgttccgat
     2521 acgtaaccag tgttaagtac gtacaggaca acattacaca cataattcac catacagctt
     2581 cccccatatg cttcactgcg tagtgtatgg agatatatgc attacacaca tacagaatac
     2641 ttagcataaa gaggagtgac attaccacgt aaagagataa gttatacaac aaacatacaa
     2701 acaatacggc acagacacac aataccataa ctccaactaa taatactaat taaccttaat
     2761 tatttatggt atatgaaata aatgtgattc tcccggaact aactaaccta accagacaga
     2821 tgatcactgt ataaaaactt cagttaatac tcatttgttt tgtcgactca gtatataatg
     2881 agtgaccagt aatcaggata atcacactta cataacctgt aaaaaagaac aagaagaata
     2941 agccactaac ataatgcaaa atggcatctg aatcgatcgc agtttggcat tcgaatcgat
     3001 catgatttgg cccaatccgt gatcgaatag agcggaatac gcaacataat tcaccagaca
     3061 agagcttaca ccgaattaca gattatgtat taatgtcaaa actggcatat tttacagata
     3121 caatgaacat gcaacccatc aatctaatag gggcggtatg tacatctgcc catgaatggg
     3181 atggcgctaa gaatacaaaa caattagaca tagatcacaa aaacacacca ttctaattga
     3241 aaaacaaaac aaacaaataa atcatactat tatagaattt tctcttgcca gacatagata
     3301 atacccctgc taattttgct aatggttagc aaaattagca gggtatataa agattattga
     3361 cccaaaacac actcagatga attcacgaca gctcaggagg gttattcctg atgtcatctt
     3421 cacaaataat ggttaacgca tcatatatca ttctgcgtct gttcctgttt tcggccttat
     3481 cgccatacaa catcatcaca tttttaattt catcgaccgg tgatttcaca tagtgaacta
     3541 atacaccaag cttctcaaca cattgcagta aagtatattt cttactgcct gtgaaaagcc
     3601 ctatcatagt ttctttacgt tcgcgactgg ataatacttt gttttccata atgacttctg
     3661 ccagcgtctt accatcataa acatactctt tatctatcag aatatttatc ttatccagca
     3721 ccttctctac gtcatcaagc aaacctgtaa tatatggggg cgcaatatat ggtttcgtac
     3781 tgatcttttc aagcatacaa ttatacatct taataacacg catcagggta aaattgttcg
     3841 catcatactg tatttcaccc tccttatata caggatcaat aagtggtatt ttttcaataa
     3901 atgcatcctg ataggctttt acatggtcaa gccaataaaa caacgagcca tactttatat
     3961 tttttctatt tactttaacc tccagtgctg tccgaagcaa cgataatgcc cccctgacat
     4021 atcctgaagg taattcacca aacaatcctt ttctttccat gtcatcataa tgctgcagca
     4081 catcattaat tttatttttc cttatcagac aaatgtacac cgcaagtgtt tcgtagcagc
     4141 tggtcacatg aacagcctca ttctgcaact gcccaaaata gcttataatc tttttcagat
     4201 atgcgtcatc acaaccattc ttatcattaa tattaaatac aagatctttc agacgggaaa
     4261 tatattcctg agatttatca tcaagtgaaa cactataacg agacaatgtt ccttctgcac
     4321 tgttttgaaa cttataaaac atttccagta atttttttgc ataaggcgtt gtatcaaagc
     4381 tggtaaagtc ataaagatat tgcgcagcgt aaccatacaa accatgaatc cagagtcctt
     4441 ctctcaccaa cactctgaga taatatgcaa ggctttgaag tatcttcgtc tttcgttgta
     4501 tatcaagtaa gacacttaat gaataaaatg cattatagaa tttatcaata ttcctgagca
     4561 ctttctccag ttcatcctta tccagcttct tctcccggta atccttttta tattcatttt
     4621 tgatagccat gatatattgt tgcatcaggc tcttgctttt cccgttatcc ttccgtatag
     4681 ttcctgtata tttccccaga gcatacagct ttccattttc atgaaaatct tccttgttga
     4741 atcccagttc agccagatat tcccgaagat aacgtttaac agcagcttca tcgctttctg
     4801 tatcagcgtg atcaagacaa ggacaatacg atttctgtaa aaaattataa atatgataat
     4861 aaaaagcaga gtcattctct ggtctgtatg tattatacat atctacaaca agaaaaataa
     4921 ccccaaaagt gcgcagacaa tagctgcgaa ggaattcagg ttttagtgtg gaatgcactg
     4981 gataagaatt catcatgtaa taaaaatgtt catatcgggc atatatgatc cgccgttgct
     5041 cctgactgaa caccccttca gcatcaataa tacgactgat ctcattataa cgttgatccc
     5101 agaatttttg atgcatttca aaattggtac tcttccgctt gtgtgccgga ttctcgcctt
     5161 ccttaaacaa ccccaaaaca gtacaaatat catacaacat atttgtaaat gtgcgcatat
     5221 gctgtgctct ccggttggtt tgcattttat cgcagaggac attctgagct taacgtatca
     5281 aactgacaca acgacatgca atgcatatgg agaacaatat gtcaaatcaa accggacagc
     5341 tttacacgcc ttcacgcatc ctgcgtatgg ctgaacttac ccgactgctg ggtatatcaa
     5401 gatctactat ttacgtgaaa ataaatccgg cctcgaaata ccacgaccct acctttccca
     5461 aaccaataag gcttggtagt gcatccgtag gctggcgggc atctgccatc gatgagtggc
     5521 tgatgttaca tacggcacca gcctggtctg aacctgaaaa caaactaaag gaacttaatg
     5581 atgacgacaa aactatcact ccctgaactt gacacaacta aactgcctga caggtgccag
     5641 gcattacttg atgaaatgca tgaagaaaca ggaatacgcc gtgaaatact tctgtctgtt
     5701 atgctgacgg tcatggctgc ctcggtccag gatacgcatg aagttgaact ttccggaggg
     5761 caacgtacca gccttcagat atttatgtgt ctttcatcag catcaggcag tggaaaaacc
     5821 tctgcctgcg caaaattaat cgctcctgtt catgaaacag aagaagaact acatcaggca
     5881 tatatcgacg ataaaaaaaa ctatgaccga atgatggaaa tgtggacaac agataaaaaa
     5941 atcctggagc ggagatataa aaaggaaatt gaaagatccc cggaaaatgc tgctgctgca
     6001 cgagcagcac ttgaaaagtg tatagcaaat aaacccgaac ctcccgtaca acaggttctc
     6061 atcgtgaatg acgcgacacc agaagggata gctttgaaac tcagccagtc accctctctt
     6121 ctgttgctgt ctgatgaagg aggtactatt ctggataagc gtttcgaacg caaatcggca
     6181 ctgtacaaca cattatggag tgggcaacca gtcaacgttg aacgggcatc cagacccggg
     6241 ttccggctga atgatgtccg tctcactata cttatactga ctcaaccggt aatatttaat
     6301 aagtttttta ctctcactgg cgatcagatc cggggcaatg gctttctggc ccggttgcta
     6361 ttttgcgaac ccggagcaat taaaataatg acgacagaac cgcatactgc cactcctgtt
     6421 gtgacgcaac aatgtgcaag ctgttttcgt aagaaaagtt ttggcagtca gatcagagac
     6481 tctctgagag catccagaga aagacgtgca aaaagtaaag agcgcatctg catgacatta
     6541 tcaaatgcag catcccgcgc actggagcgc tttcacaaag aaaatatgga tactgtcaga
     6601 cagaatcctc atatgggtac atttgaagac attattgtca gaaaaagaga gcaggttgtc
     6661 aggatagcag cacttctgga actggagaaa gatccagact cgacagtaat aacgctggaa
     6721 agtactaata gcgccattta tctcattgaa ttttatttca agcatcttat atataaactt
     6781 gaatcccttc gggaaatatc cccggctgag aaacttgata aatggcttca gaaaagaatt
     6841 atcacaacag caggttacat attccagaaa agttatattt tacaatatgc cccatatgct
     6901 ctcaggaaaa aatgtgttct ggatgaagca cttgacatac tggcggaaca aaggaaaata
     6961 aggattgatg ataacctggt tgtttatata ggaaacacga ttacaccatc cgaactggca
     7021 aaaaaactaa atattcctgc attcgatgcc ggagttttca tttgtgatca tcaaaacata
     7081 ctgaagtatc accgaaacag actatagata ctatcggaca aaaaataaat tatcacaagt
     7141 gtattattcg ttataataat gaataatcgt tatctgagtg caatgccagg ttttcattac
     7201 gaactggcat tgcatttatt tttcaaaaaa gaaagcagta accatttggc tactctgagt
     7261 atgccagtcc atgaaactaa gcgaattcct tagtgttttt agcaagttga gacatcacat
     7321 aatccgccca tgcctgcatc atcggtacac gctgctccag tagatcagta cgatgatatg
     7381 ccgcctcaac cttatttttc agcgtatgag cgagcgccct ttccgccaga tcccgcgaat
     7441 acccctgttc actacaccag tccctgaatg ttgagcgaaa accatgtgcc gtggcaactc
     7501 gccccggaat gtcactgacg gctttctttt tacgcagaaa acttgtcaac accatatcgg
     7561 aaaggatctg ctgctttctg ggtgaaggga acaccagttc atcatgcagg ccacgtatat
     7621 tttccagaat gtaaatagcc tgccgggata aaggaacacg atgctgtagc ctagctttca
     7681 ttctttctgc aggtatagtc catacccgct tatgaaaatc aatttcagcc cagcgcattc
     7741 ccctggcttc gcccgagcga gttgctgtaa gtatcaccat taataacagt gcgcgggtaa
     7801 cattataagg ttcatcacta tacacactgg tcgccacaaa aagcggtaac tgcctccagg
     7861 gcattgcggg ttggtgttca tcacgtcctc ttgtctgctg aggaagcaaa tggtcaacca
     7921 catcaacagg atttgccaca caaaaaccgt gcgcccatcc ccactgcata acaacatgaa
     7981 tgcgctgttt aacccggctt gccgtttctg acaaggttaa ccagactgga cgcagtgttt
     8041 ctgccacgtc cgcagccgta atcgaatcca gcgtttttgc tcccagttga ggaaacgcgt
     8101 aattctcaag cgtcgataac cactgcctta catgctttgg attctcccat ccaggagaca
     8161 gttctgcatg tacacgcctg gctgcatcgg caaatgttgg gataacgact ttctcagatt
     8221 cagccttttt aatctccaga ggatcatcac ctgcagcaag ttgctctcgc attatccggg
     8281 cagtacgtgc agcttcagca atactgacct ctgggtaagt tcccaatcca gcattacgtc
     8341 ttttttgtgt caccggactt acataacgaa aaacccattt cccccgcccc tttactgaag
     8401 aaggatgaag ggtcagtccg gtaattcccc catggggcaa tggtttgtca tcaggtttga
     8461 tatgtcttgc tttcgtatcc gtcaatactg ccatacgcta attcccgtca ctctggtatg
     8521 ccatccagta tgccattagt ccctcgcttc actaagaata cattagataa catcggacaa
     8581 caaaaagtgt aatacattgt ttataaagaa attatataac acccctggat taagtcagat
     8641 ttatttcagg cggtccccct caccgccata tttaaagaag agcgcgtacg aaagtacggg
     8701 cttttttttc gtatattgta cacaccgggt gcccccgccc acgtctgtta gggcgagggg
     8761 aaattgtgca catcaactgt ctggttactc agtcaacaat caaaatgcgt aagatagtac
     8821 agaacatata tgcgttatct ggtcacttcc tgtaacttcc tcacttatta aaatgccccc
     8881 aacggaaata catgtcacat gccatcataa aatcgttatg ccagaggaat cgacaatgga
     8941 aaaaatagta aattaagcac ggtacatagc agagtaaaaa ataggcttca aaccaaatcc
     9001 gccattccac aagcgttgcc aataccgaca ccagcgtaat ccacatcatt tcaataaccc
     9061 gcaatatgaa taaccgaagc caaagcggct tggcaaaaaa ggtcgacaac gtattttcac
     9121 tggagaaaaa cagatcgatt tcctgcaaaa aaaacctgcc cagaaaagcg ggccgcttgg
     9181 agaagcgcag aaaaaacaga ttaatcgcca acactacgcc acaaagaatg agcaatgcaa
     9241 ttatcgattt aaacgttaac cactgcccct ctgtcacaaa agaatcactg ttacgcagcg
     9301 ggctgatagt aagcaggtta acaaccgttg agatgcacac cgaaagcagc acatagcgag
     9361 tcagactttt atattttact tttttcgcta ctgaaatagc gacctgcttg acataactgg
     9421 tgcggatcat ccgaaacagc agaaagatga ccaacggccc aagtaacgaa atcaggaaca
     9481 gcgcatataa cgagggtaga agctggtata gcccccaact cattgcactc aacatgatta
     9541 acgacagaaa tcctgccacc agaggttgtg taagaaataa ttctattttg tggttctgct
     9601 ttttcttaaa ccacgaaaaa tcgatattct tcgtgacgca tttataagcc cagtacgcct
     9661 tgagatcaaa aataaaatta tacagcacca gggccagaat cccactaact acaccaatat
     9721 cctctgatgg aaaagtaaac cctgcatttt tccaataaaa gcctatcaga ataagtccaa
     9781 tagcataaat gtatatcaga tttcgtttat cctgaaagcg aataaaagtt aaataatcag
     9841 gaatcatttg aatacctttg catcactaaa tgctttacga caatgcataa ctcgttgctg
     9901 atccacgcca gcaaaatcat ttaacatatt ccccaaatag ggcttattac ttatttcaag
     9961 gaaagctacc ggggttggca tattcagaat atgtatcgag agattcagtc catttcaccg
    10021 tatgagttaa gtgtaatgag agattttctt tgataattga cggagcgacc tcgctttttg
    10081 ctgttacgtt catcagaacc tgatgctctg gcgaagcgat atccagtccg gcaagataat
    10141 cgcgcattgc ctgaacgccg tcttccatca aacgcgtgtg ccaggcaccg ctcacaccga
    10201 gtttaaccgg ttcgtatcca gcggccatca gcagcgtggt aaattcattc aacgcagcct
    10261 gcgttccccc aataacctgc tggcgcggcg tgttatcaca gctaatatcc agcgcaatgc
    10321 ctgattccgt aatcatcgtc tgcagttgtt cgcggttgat gcctttaacc gcctgatctt
    10381 acccagcaat agtggacacg cggctaagtg agtaaactct cagtcagagg tgact
//
LOCUS       DAEBXN010000134        10424 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.134, whole genome shotgun sequence.
ACCESSION   DAEBXN010000134 DAEBXN010000000
VERSION     DAEBXN010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10424
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.134"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            2..1369
                     /gene="dcuC"
                     /locus_tag="KJL99_003613"
     CDS             2..1369
                     /gene="dcuC"
                     /locus_tag="KJL99_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HBD4438199.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASCAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMQSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFVNVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMPFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
     gene            complement(1412..1909)
                     /gene="sspB"
                     /locus_tag="KJL99_003614"
     CDS             complement(1412..1909)
                     /gene="sspB"
                     /locus_tag="KJL99_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HBD4438200.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(1915..2553)
                     /gene="sspA"
                     /locus_tag="KJL99_003615"
     CDS             complement(1915..2553)
                     /gene="sspA"
                     /locus_tag="KJL99_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HBD4438201.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(2948..3340)
                     /gene="rpsI"
                     /locus_tag="KJL99_003616"
     CDS             complement(2948..3340)
                     /gene="rpsI"
                     /locus_tag="KJL99_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBD4438202.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(3356..3784)
                     /gene="rplM"
                     /locus_tag="KJL99_003617"
     CDS             complement(3356..3784)
                     /gene="rplM"
                     /locus_tag="KJL99_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HBD4438203.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(4003..5130)
                     /gene="zapE"
                     /locus_tag="KJL99_003618"
     CDS             complement(4003..5130)
                     /gene="zapE"
                     /locus_tag="KJL99_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HBD4438204.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGVKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            5324..5722
                     /locus_tag="KJL99_003619"
     CDS             5324..5722
                     /locus_tag="KJL99_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HBD4438205.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQVPRDYSEGASGLLRTGAKRD"
     gene            5876..7223
                     /gene="degQ"
                     /locus_tag="KJL99_003620"
                     /pseudo
     CDS             5876..7223
                     /gene="degQ"
                     /locus_tag="KJL99_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
     gene            7313..8380
                     /gene="degS"
                     /locus_tag="KJL99_003621"
     CDS             7313..8380
                     /gene="degS"
                     /locus_tag="KJL99_003621"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HBD4438206.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(8442..9380)
                     /gene="mdh"
                     /locus_tag="KJL99_003622"
     CDS             complement(8442..9380)
                     /gene="mdh"
                     /locus_tag="KJL99_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HBD4438207.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKNCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGKEFVNK"
     gene            9815..10285
                     /gene="argR"
                     /locus_tag="KJL99_003623"
     CDS             9815..10285
                     /gene="argR"
                     /locus_tag="KJL99_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HBD4438208.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
BASE COUNT         2552 a         2654 c         2611 g         2607 t
ORIGIN      
        1 aatgttcggc ataattatat ctgtcatcgt attaattacg atgggctatt tgatcctgaa
       61 aaactacaaa cctcaggtgg tgctggctgc cgcaggtgtc ttcctgatga tgtgcggagt
      121 ctggttaggg ttcggtggtg tactcgatcc cgccaaaagc agcggctact tgatcgtcga
      181 catttataat gaaatcctgc gcatgctgtc caaccgcatt gccggattgg ggctgtcgat
      241 tatggcggtg ggcggttatg cccgctacat ggagcgcata ggagccagtt gcgcgatggt
      301 gagcttgcta agccgcccgt taaaactcat tcgctcgccg tatattattc tgtcggcaac
      361 ttacgtcatc ggccaaatca tggcgcagtt tattaccagc gcctccggtc tgggtatgtt
      421 gctgatggtc accttatttc cgacgctggt gagtctggga gtaagtcgcc tctctgcggt
      481 ggcggttatc gcaaccacga tgtccattga gtgggggatt ctggaaacga actccatttt
      541 tgcagcccag gtcgcgggaa tgaaaattgc cacatacttc ttccactacc agcttccggt
      601 cgcctcttgc gtcattatct cggtggcgat ctcccacttt ttcgtgcaac gcgcttttga
      661 caaaaaagat aaaaatatca atcacgaaca ggcagagcaa aaaactctcg ataatgtccc
      721 gccgctctat tacgccattt tacctgtgat gccgttaatc ctgatgctcg gctcgctgtt
      781 cctcgcccac gtcgggctga tgcagtcaga actgcatctg gtggtggtga tgttactaag
      841 tttgacggtg acgatgtttg ttgagttctt ccgcaagcat aacttgcgcg aaacaatgga
      901 cgatgtgcag gcgttttttg acggcatggg tacgcagttt gtcaacgtgg taacgctggt
      961 ggtcgcgggt gaaatatttg cgaaaggctt aacgacgatt ggcactgttg atgcggttat
     1021 caggggtgcg gagcattctg gtctgggcgg tattggcgtg atgattatta tggcgctagt
     1081 cattgccatt tgtgccattg tgatgggctc tggcaatgcg ccgtttatgc catttgccag
     1141 tcttattccg aatatcgcag ccggactaca tgtaccagcg gttgtaatga ttatgccgat
     1201 gcattttgcc acgacgctag cgcgcgcggt ttcgccgatt actgcggtgg tggtcgttac
     1261 gtcaggaatt gcaggcgttt cgccttttgc ggtggtgaag cggacagcga tccccatggc
     1321 agtcggtttc gtggtgaata tgattgccac aatcacgcta ttttattaag tcattaaaaa
     1381 gacaaaacag gccgcctggg cctgttttgt attacttcac aacgcgtaat gccggtcgac
     1441 caccgcgtgg tggctgcgga ggttcatcgt caggatgagt gtcatcatcg tgatctggct
     1501 tgtcgccatc aataaccgac ataacggttt cgttgtctgc cgatgcctct tcatcattca
     1561 tgatgctggt atcttcatcg taggcagctt caggctcaaa catcgtgcct gcgccatttt
     1621 cacgggcgta gatagccagc acggcagcca gcggcacaga aacctgacgc ggaatgccac
     1681 caaagcgcgc gttaaagcgc acctcatcat tcgccagttc cagattgccg acagcacgcg
     1741 gcgcaatgtt gagtacgatt tgcccgtcac gcgcatattc cataggaacc tgcacgccag
     1801 ggagcgtcac atccaccacc aggtgcggcg tgagctggtt atccagcaac cactcataga
     1861 atgcacgcag cagataggga cgacgtggtg ttagctgtga caaatccata cagattaact
     1921 ccggcccaga cgcatttcac gttctgcttc agttaaagaa gcaaggaaag agtcacgctc
     1981 aaagacgcgg gtcatatagc ctttcagctc tttcgcaccc gggccgctga actcgatgcc
     2041 cagttgcggc agacgccaca gcagcggagc aaggtagcaa tcgaccaggc tgaactcatc
     2101 gctcaggaag tacggcttct gaccgaagac cggcgcaatc gccagcagtt cttcgcgcag
     2161 ttgcttacgt gcggcatctg cttcagaagc tgaaccgttg atgatagtgt tcatcagcgt
     2221 gtaccagtct ttttcgatgc gatgcatgta cagacggctt tcaccgcgag ctaccgggta
     2281 aacaggcatc agtggcggat gcgggaaacg ctcatccaga tattccataa tgatgcgaga
     2341 ttcccacagg gtcagctcac gatccaccag ggtcggaacg ctctgattcg ggttgaggtc
     2401 aatcagatcc tgaggcggat tgtccttttc cacgtgttcg atctcgaaac ttacaccttt
     2461 ctcagccagc acaatgcgga cctgatggct atagatgtca gtaggaccgg aaaacagcgt
     2521 cattaccgaa cgtttgttgg cagcgacagc catgaaaacc tccaggtata gtcagaattt
     2581 ttactgctac cagccaccag gtggccagtc agaagttgtg ttacccaata aggaacgact
     2641 ctctttgttc gaaaatcaaa caaaaaatga gcaatacccg acatttgggc agaaaattgg
     2701 atgatagttt accagatttt gcgacctttg tggtgagtgg atgccggaaa tggggaaaaa
     2761 gagatgcgct ttagtctgaa atagttgact tagaccctta ttggcgatgt ggtttttgtt
     2821 ttacctgtct ttcagacggc agcaaaaagc aacttcccat tttttacgct gattcagaat
     2881 ttagccataa aaaaacccgc cgaagcgggt tttttcgcaa attgttttct gccgtagcag
     2941 aagccaatta acgtttggag aactgcggac gacgacgtgc tttacgcaga ccgactttct
     3001 tacgttcaac ctgacgagcg tcacgagtaa cgaagccagc tttacgcagt tcagaacgca
     3061 gggactcgtc gtattccatc agagcgcggg tgataccgtg acggatcgca ccagcctgac
     3121 cagagatacc accaccttta acggtgatgt acaggtccag tttctcaacc atgtcgacca
     3181 gttccagcgg ctgacgaact accatgcggg cagtttcacg accgaagtac tgttccagag
     3241 aacgttggtt gattacgatt ttaccgttgc ccggtttgat gaaaacgcga gctgcggaac
     3301 ttttgcggcg accagtgccg tagtattgat tttcagccat tgcctataat cccgattaga
     3361 tgtcaagaac ttgcggttgc tgtgccgcgt ggttgtgctc gttacccgcg taaactttca
     3421 gtttacggaa catagcacga cccagcgggc cttttggcaa catgccttta accgcgattt
     3481 caatcacacg ctcaggacgg cgagcaatca tctcttcaaa ggtcgcttgt ttgataccac
     3541 cgatgtggcc ggtgtggtga tagtacactt tgtcagtacg cttgttgccg gttacagcaa
     3601 ctttgtcagc gttcagaacg atgatgtaat caccggtatc tacgtgcgga gtatattccg
     3661 ctttgtgctt accgcgcagg cgacgagcca gttcagtagc cagacggccc agagttttac
     3721 cggtcgcgtc aacaacatac cagtcgcgtt ttacggtttc tggtttagct gtaaaagttt
     3781 tcattaaaag cttacccaat aaatagttac acgttggtga acacccaaac gtcttcaatt
     3841 gttgaggttc acacgacaaa gtccggcaaa cctacccctt cgaatagcct atgccagcac
     3901 acaaaaagtt ttgggaaaaa aactttcttg taacgtgggg tcgcaggatt atagagaagt
     3961 cggggtcaaa gatcgacccc tttttgtgat ttgtggcagg ttttaacccg ccaaatgctc
     4021 gcgcttcaga tactcttcgc tttgcatctc ttgcagacgt gacaggcaac gctggaactc
     4081 aaacttcagc cgctcaccct gataaatttc atacagcggc acttctgcac tcaccactaa
     4141 tttgacatgg cgctcgtaaa actcatccac cagcgcaata aagcgccgcg cttcgctctc
     4201 catcaaccgc gtcatgactg gcacatcaaa caacattact gtgtgaaaca ggcgtgagag
     4261 cgcaatatag tcatgctgac tgcgggcgtc gacgcacagc gtagtaaaag aaaccgccag
     4321 cgtctggttc tcgacgccca ttgtcgccaa tggccgatgg ttgatttcta acgtcggtga
     4381 attttctcgt ttcacccccg ccagcgccaa ccatagttta tccatctgcg cccgggtttc
     4441 atcgttgagt ggcgaaagcc acagatgcgc ctgagtgagt gtacgcagtc gataatcaac
     4501 accagcgtcc acgttcatta catcacaatg ctgtttaatg gcatcgattg caggcagaaa
     4561 acgcgcacgt tgcaggccat ttcgataaag ttcgtccggc ggaatatttg acgtcgctac
     4621 cagggtaata ccgcgggtga acagggcttt catcagaccg ccaagtagca tggcgtcggt
     4681 aatatcagaa acaaaaaatt cgtcaaaaca gagcacgtca gtttcggctt taaagcgatc
     4741 ggcaataatt tccagcggat cggtctgccc ctgtaaggca gttagctctt cgtgcacccg
     4801 cagcataaaa cggtgaaagt gcaggcgctg tttccgctct cccggcaggc tttgatagaa
     4861 aaggtccatc agccaggttt tcccgcgtcc tacaccgccc cacatatata agccacgcac
     4921 tggcgtatgc tttgtgtctt cgcgtttacc ccacagctta ccgacccgcg ccattagccc
     4981 actcgtcctg ggggctggtg gcctgctatt gatgagttct tgataaataa tttccaggcg
     5041 gctgacggcc tctttttgaa cgtcgtcggg ttgatggctg ccttcattga gcgccttcag
     5101 gtattgcgat gttggggtaa cgctttgcat gatcttattg ttattccttg aataatcggt
     5161 gcgccgttgt tcacggttga cgaaaaaaag gccgttctac actacgcgat atgcagtcgg
     5221 gattccactt ctgtggaatt aacggttata gtggcataat cagccgcagg catggagcct
     5281 gaagccaaca ccctacggaa acaaaagaca acgggagatg ttcatgacct gggaatatgc
     5341 gctaattggg ttagtcgtcg gcatcattat tggtgctgtg gccatgcgtt ttggtaatcg
     5401 taaactacgc cagcaacagg cgttgcagta cgaactggaa aagaataaag ctgaactgga
     5461 cgagtatcgc gaagagctgg ttagccactt tgcccgcagc gcggaattac tggataccat
     5521 ggcgcacgac tatcgccagc tgtatcagca catggcaaaa agctccagca gcctgctgcc
     5581 ggaactgtct gctgaagcaa acccgttccg taatcgtctg gcagagtctg aagccagcaa
     5641 cgatcaggca ccggtgcagg tgcctcgcga ctattctgaa ggcgcatccg gcctgctgcg
     5701 tactggcgcg aagcgcgact aatttatttt tcgggcgcag ccattgcgcc ctcctcttct
     5761 ctccctcccc gactatcatt taatctggtg tctcattgtt agccgtctga aaattcaata
     5821 acatcaaact gttttgaatc tcttttctta tcattcaggt acgagagcag gaataatgaa
     5881 aaaacaaacc cagctgttga gtgcattagc gttaagtgcc gggttaactc tctcggcgtc
     5941 atttcaggcc gtcgcgtcga ttccaggcca ggttgccgat caggcccctc tccccagtct
     6001 ggctccaatg ctggaaaaag tgcttccggc agtggtgagc gtacgggtgg aaggaacggc
     6061 cagtcaggga cagaaaatcc cggaagaatt caaaaagttt tttggtgatg atttaccgga
     6121 tcaacctgca caacccttcg aaggtttagg ttccggtgtc atcatcaacg ccagtaaagg
     6181 ctatgtgctg accaacaacc atgtgattaa tcaggcacag aaaatcagta ttcagctcaa
     6241 tgatgggcgc gagtttgatg caaaactgat tggtagcgat gaccagagcg atatcgccct
     6301 gttataaatt caaaacccga gcaaattaac gcaaatcgct attgccgact ccgataaatt
     6361 gcgcgtcggt gattttgccg tagcggtcgg taacccattt ggccttgggc aaaccgccac
     6421 ctctggcatt gtttccgcat taggccgcag cgggttgaat cttgaaggtc tggaaaactt
     6481 tatccagaca gatgcttcca ttaaccgcgg taactccggc ggtgcactat taaaccttaa
     6541 cggtgagtta attggcatca acactgcaat ccttgcgcct ggcggcggga gcgtcgggat
     6601 tggatttgcc atccccagta atatggcgcg aacactggcg cagcagctta tcgactttgg
     6661 tgaaatcaaa cgcggtttgt taggcatcaa aggcaccgag atgagtgccg atatcgccaa
     6721 agccttcaac cttgacgtgc agcgtggcgc gtttgtcagc gaagtgttgc caagttctgg
     6781 ctcagcgaaa gcgggcgtca aagcgggcga tattattacc agcctcaacg gcaaaccgct
     6841 gaatagcttt gctgagttgc gctctcgtat cgcgactaca gagccgggca cgaaagtgaa
     6901 gcttggcctg ctgcgtaacg gcaaaccact ggaagtagaa gtgacgcttg ataccagcac
     6961 ctcttcgtcg gccagcgctg gaaggtgcaa cgttgagcga tggtcagcta aaagatggcg
     7021 gcaaaggtat taagatagat gaagttgtca aaggaagccc agctgctcag gctggcttgc
     7081 aaaaagacga tgtgatcatt ggcgtcaacc gcgatcgggt gaactcgatt gctgaaatgc
     7141 gtaaagtgct ggcggcaaaa ccggccatca tcgccctgca aattgtacgc ggcaatgaaa
     7201 gcatctatct gctgatgcgt taatgtcgta aaccgggcat caggctgacg tgtgatgtcc
     7261 ggttaactcg tggtatgctg ctgccgttcc cttttttaat gacgcctcca tcatgtttgt
     7321 gaagctctta cgttccgttg cgattggatt aattgtcggc gctattctgc tggttgccat
     7381 gccttcgctg cgcagcctta acccgctttc cactccgcaa tttgacagta ccgatgagac
     7441 gcctgccagc tataatctgg cggttcgccg cgccgcgcca gcggtggtta acgtttacaa
     7501 ccgtggtttg aacaccaact ctcacaacca gcttgagatc cgcaccctgg gatccggtgt
     7561 aatcatggat caacgcggtt atatcatcac caataaacac gtcatcaacg acgccgatca
     7621 gatcatcgtc gccttacagg atggacgtgt atttgaagca ttgctggtgg gatctgactc
     7681 tctaaccgat ctggcggtac ttaaaattaa tgccactggc ggtttaccta ccattccaat
     7741 taatgcacgt cgcgtaccgc acattggcga cgtagtactg gcgatcggta acccgtacaa
     7801 cctcgggcag accattaccc aagggattat tagtgccacg ggtcgaatcg gtctgaaccc
     7861 gaccgggcgg caaaacttcc tacaaaccga tgcttccatt aaccacggta actctggcgg
     7921 cgcgctggtg aactcgctgg gagaactgat gggcattaac acgctgtcgt ttgataagag
     7981 taacgatggc gaaacgccgg aaggtatcgg ctttgcgatt cctttccagt tagcaaccaa
     8041 aattatggat aagctgatcc gcgatggtcg cgtgatccgc ggctacattg gtatcggcgg
     8101 acgcgagatc gcaccactgc acgcgcagag cggtggtata gatcaactgc aagggatcgt
     8161 ggttaatgaa gtgtcacctg acggcccagc ggcgaatgcg ggtattcagg tcaacgatct
     8221 gattatttcg gtggataaca aaccggccat ctctgctctg gagacgatgg atcaggtggc
     8281 ggaaattcgc cctggttcgg tgatcccggt tgtagtgatg cgtgatgata agcagttaac
     8341 gctgcaggtc accattcagg aatatccggc aaccaattaa gtcgagtgct caaaacaaaa
     8401 accggagtct gtgctccggt tttttattat tcgctaatca attacttatt aacgaactct
     8461 ttgcccaggg cgatatcttt cttgagcgta tccagcatac cttccagcgc gttctgttca
     8521 aatgcgctca gggtaccgat agatttacgc tcttccacgc cgtttttacc cagcagcagc
     8581 ggttgagaga agaaacgggc gtactgaccg tcgccttcaa cgtaggcaca ttcgacaacg
     8641 ccttgttcgc cctgcagtgc acgaaccaga gacagaccaa aacgtgcagc tgcctggccc
     8701 atagacaggg ttgcagaccc gccaccggct ttcgcttcaa ccacctcagt acccgcgttc
     8761 tggatacgtt tggtcagatc agccacttcc tgctcggtaa aactaacgcc aggaacctgt
     8821 gacagcagcg gcagaatggt aacaccagag tgaccgccaa taaccggcac ttcaacttcg
     8881 cctggctgtt tgcctttcag ttccgcaaca aaggtgttgg aacgaatgat atccagcgtg
     8941 gtaacgccga acagtttgtt tttgtcataa acaccggctt ttttcagcac ttcagcagca
     9001 attgcaactg tggtgttaac cgggttagtg ataataccaa tgcacgcttt cgggcagttt
     9061 ttcgcaactt gctgtaccag gtttttcacg atgccggcgt taacgttaaa caggtcggaa
     9121 cgatccatac ccggtttacg cgctacgcct gcagagataa gaacgacatc tgcgccttcc
     9181 agcgccggag tcgcatcttc accagaaaaa cctttgattt tcacagcagt agggatatgg
     9241 ctcagatcga cggccacacc gggagtcact ggagcgatat catacagaga gagttctgaa
     9301 cctgaaggca gttgggtttt taacagtagt gcaagcgcct ggccaatacc gccagcagcg
     9361 ccgaggactg cgactttcat cctaaactcc ttattatatt gataaactaa gatatgttgc
     9421 tccgctgccg cgaccttaat ccacaaaatt gccgtttaca atgaccacat ctcaagaatg
     9481 tgtagtcacg caagtttagc gtttatgcat ttaattgccg taatcaggaa cctaacttac
     9541 gtaattaaca gtcgcttatg cattagcgca acattccaac aggtggtgac aatataccct
     9601 accgttcagc caaaacaaca tcaatttgat aacaattaat ttacttttaa acagaatttg
     9661 catgccgtga cgcaggcatg tttctcaata acgaaatttg ataaaatccc gctctttcat
     9721 aacattattt cagccttctt cagggctgac tgtttgcata aaaattcatc tgtatgcaca
     9781 ataatgttgt atcaaccacc atatcgggtg acttatgcga agctcggcta agcaagaaga
     9841 actagttaaa gcatttaaag cattacttaa agaagagaaa tttagctccc agggcgaaat
     9901 cgtcgccgcg ttgcaggagc aaggctttga caatattaat cagtctaaag tctcgcggat
     9961 gttgaccaag tttggtgctg tacgtacacg caatgccaaa atggaaatgg tttactgcct
    10021 gccagctgaa ctgggtgtac caaccacctc cagtccattg aagaatctgg tgctggatat
    10081 cgactacaac gatgcagttg tcgtgattca taccagccct ggcgcggcgc agttaattgc
    10141 tcgcctgctg gactcactgg gcaaagcaga aggtattctg ggcaccatcg ctggcgatga
    10201 caccatcttt accacccctg ctaacggttt caccgtcaaa gacctgtacg aagcgatttt
    10261 agagctgttc gaccaggagc tttaatctct gccccgtcgc ttctggcggc ggggaaaatg
    10321 ttgcttatcc ctctcattcc ccttctttct cctgcgatac atttaacgaa tagtgcgttt
    10381 taccgcgaca catcattcac aaaatgaata catacggtaa aaaa
//
LOCUS       DAEBXN010000135        10028 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.135, whole genome shotgun sequence.
ACCESSION   DAEBXN010000135 DAEBXN010000000
VERSION     DAEBXN010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10028
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.135"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(13..1227)
                     /gene="rspA"
                     /locus_tag="KJL99_003624"
     CDS             complement(13..1227)
                     /gene="rspA"
                     /locus_tag="KJL99_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HBD4438209.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPRLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNR"
     gene            complement(1433..1759)
                     /locus_tag="KJL99_003625"
     CDS             complement(1433..1759)
                     /locus_tag="KJL99_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HBD4438210.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLSLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            1894..2235
                     /locus_tag="KJL99_003626"
     CDS             1894..2235
                     /locus_tag="KJL99_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HBD4438211.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            2301..2830
                     /gene="speG"
                     /locus_tag="KJL99_003627"
                     /pseudo
     CDS             2301..2830
                     /gene="speG"
                     /locus_tag="KJL99_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
     gene            complement(2833..3543)
                     /locus_tag="KJL99_003628"
     CDS             complement(2833..3543)
                     /locus_tag="KJL99_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HBD4438212.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            3651..3956
                     /locus_tag="KJL99_003629"
     CDS             3651..3956
                     /locus_tag="KJL99_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HBD4438213.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYNRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            4155..4475
                     /locus_tag="KJL99_003630"
     CDS             4155..4475
                     /locus_tag="KJL99_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005091980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase"
                     /protein_id="HBD4438214.1"
                     /translation="MSKNDRMVGISRRTLVKSTATGSLALAAGGFSLPFTLRSAAAAV
                     QQASEKVIWGSCSVNCGSRCALRLHVKDNEVTWVETDNTGSDEYGNHQGTRLFARSLH
                     PPAY"
     gene            5035..5613
                     /locus_tag="KJL99_003631"
                     /pseudo
     CDS             5035..5613
                     /locus_tag="KJL99_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606644.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(6295..7284)
                     /locus_tag="KJL99_003632"
     CDS             complement(6295..7284)
                     /locus_tag="KJL99_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438215.1"
                     /translation="MTMIKDSYQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQDNIPFPVEVLPTRNSLPTPIIAHTLSDRSVVVPVRGGERPEVVT
                     VPSGQEIVVEPIEQAILISEQTKLWDAKSSTGFTHGTLQQDAMNICENVVRTINARMV
                     DVLESSKLLKTVELPVLTGSLTTKADAIMDALFENTESSFGSEVSDYGIIAHESQLKA
                     LSRLAAKQGFSGEDAIVDMLGTDIAYYNGEDKGVFMLAKRFTALSFGCFRHDGENITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTVKMGTGSATAVLPVVKRLKFTKTEA"
     gene            complement(7361..7852)
                     /locus_tag="KJL99_003633"
     CDS             complement(7361..7852)
                     /locus_tag="KJL99_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438216.1"
                     /translation="MLFNNNDWKLSVTDINLYENTVSLDGQSYPLSFAIKTLIPGYLS
                     GLPATSRESMELLEALAEAGVAIGNFFSNDLMTAYQRRQMNKRAEAERIAKEQRLQAE
                     RMREENMTDAEWQRELQRREQVKAERRTYGENLHSATHSAGRSRAAIVADIESGGNWM
                     DSL"
     gene            complement(8171..8668)
                     /locus_tag="KJL99_003634"
     CDS             complement(8171..8668)
                     /locus_tag="KJL99_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438217.1"
                     /translation="MTSDNKQEYTIHIFGQDITSQDGLFRLDEIRRVGIQIGKVEDTE
                     STQYKRFAQTKVGKLLRNRQKIRLIRMGELGSTVLADPAATVEFGRWLDFEYGLAVSE
                     AFVSMTSSATVQSAVASAEDIKPETKEFIQRHGTGLYIAGREVSQAEYSDYQAVTAGR
                     CEWGK"
     gene            complement(8658..9173)
                     /locus_tag="KJL99_003635"
     CDS             complement(8658..9173)
                     /locus_tag="KJL99_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707480.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438218.1"
                     /translation="MNTELPVTNRQFLIILLTNAESSVDCCRYNLDLLKLSRWYAQAE
                     DWDYEKIREMYAEPIQSYLQTHTEEELKRGLQDIFYFNNPYTCEGDPEEMDWIASRSD
                     ADLKHISNAMMQVESKWSRRLQVLTEDYENGTFDKLVPPDTERLQAKADQEQRFKSFA
                     EALAGGASYDF"
BASE COUNT         2560 a         2536 c         2507 g         2425 t
ORIGIN      
        1 ttatcttact ccttacctgt tccacagcgt gccatcttcc agacgtgcga ctggtagata
       61 agcaggttca tagggatatt tcgccgccag cttttcatcg aattcgatac caagacccgg
      121 tttgtctccc ggatgcatat agccgttatc gaaagtccag ttgtgcggga agacttcgag
      181 catttgttcg gaataaccca tgtattcctg gacaccgaaa ttggggaccc acagatcaaa
      241 gtgcagcgcc gcagccatgc agactggtga caaatcggaa ggaccgtgtg agccagtacg
      301 tacctgatac agcgaagcaa aatcggcaat ccggcgcata ccggtaattc cgcctgcatg
      361 ggtcagcgtg gtgcggatat aatcgatgag ttgctcttca atcagttgtt tgcagtccca
      421 gatgctgttg aagacttcac ccactgcgat gggtgtgacg gtatgttggc gaatgagacg
      481 gaagcattct tggttttccg ctggcgtcgg gtcttccatc cagaacatgc gataatcttc
      541 aatgctttta ccaaagcgcg ccgcttcaat aggcgttaag cgatggtgca tgtcatgcag
      601 caaatgttca ttaaaaccaa acttgttacg taccgcgtca aacaatttcg gcatgaaatc
      661 gaggtatttc tccgtcgacc acagctgctc ttccggccac tgtcctttgg ttgcgggttc
      721 ataagccaga cctttacctt tcgacatgcc gtaggtggtt ttcataccag ggattccgca
      781 ctgcacgcgg atggctttga atcccagctc ctgatgacgg gcataatcat ccagagcttc
      841 atcaatactg tgaccggtgg tatggcaata aaccatcacc ccttcacgag acgcgccgcc
      901 gagtaactgg taaagcggca tgttggcagc tttggcttta atatcccaca gcgccatatc
      961 aaccgctgaa atggccgaca tcgtaaccgg accgcgacgc cagtaagcac ctttatagaa
     1021 aaactgccag atatcttcga tacggtgcgc atcgcgacca ataagccgcg gacaaagatg
     1081 atcctgcaaa taagaggcca cggaaagctc acgtccattg agggtggcat ccccaaggcc
     1141 cgtaataccg tcctcagtgg tgatttttaa tgtgacgaaa ttacgccccg gacaggtaac
     1201 aaaaacttca gcctttacga tcttcatgtt cgattccttg catcgcttgt cgtgatgcat
     1261 gaaatctacg caactgagct actgccatac aagtatgaag atcgaaaaaa gccggagtga
     1321 tcacaaaaaa aggcgtatat ttgcgctgtg aatggttgac aaaagatgaa atagaatacc
     1381 ttttgtcagc tgacacttcc tcgtatctta ttgataaaat ggatttatgt tcctacgtgc
     1441 gcccccagcc cgcaacaatg atcaacattc cgcaaagcgc aatcaacgca cccgtccagt
     1501 cataaagact tagtttcacg ccatccacaa cgcgcagcca catcaatgcc gtgcagacat
     1561 aaacgccacc ataagccgcg taaacacgcc cactcgccgc aggatgcaac gttaacaacc
     1621 agacaaacag cgccagtgaa atccccgccg gaagcaacag ccagatactg gcgtttcgtt
     1681 ttaaccacaa ccagggcaga aagcatccaa taatttcaca cagcgcagta gcaaaaaata
     1741 gtaacgttgt tttaatcatc tttgtcactt attgacatca tgtatagtta tagggcgaca
     1801 taatatcatc aatataaaca tcctcctggt acgttttgcg ttcgcagtgg atggtgtaga
     1861 atcaccttta atcattcata cagggaatga attatgaaaa tcactctcag caaacgaatc
     1921 ggcctgctcg cttttctgct gccttgcgca ctggcattga gcacaactgt tcatgccgaa
     1981 actaacaaac tggtgattga gtctggcgac agtgcacaaa gccgccagca cgccgctatg
     2041 gaaaaagagc aatggaatga cacgcgcaat ctgcgccaga aagtgaataa acgcactgaa
     2101 aaagagtggg ataaagccga cgccgctttt gataaccgcg ataaatgtga gcaaagcgcc
     2161 aacatcaatg cctactggga gcccaatact ttgcgctgcc tggaccgtcg aactggccgc
     2221 gttattaccc cctaacctgt tattgattta aggaatgtaa ggacacgtta tgccaagcgc
     2281 ccacagttaa gctacgcccg ctggagcgtg aagatttacg ctatgtacat caactcgaca
     2341 ataacgccag tgtgatgcgt tactggtttg aggaaccctg cgaagccttt gttgaactct
     2401 ctgatctgta tgataagcgc atattcacga tcagagcgaa cggcgctttg tggtggaatg
     2461 tgacggcgaa aaagccggtc tggtggagct ggtggaaatt aaccatgttc atcgccgggc
     2521 agaatttcag ataattatct ccccggagta tcaggggaaa ggtctggcaa cccgtgccgc
     2581 caaattagca atggactatg gcttcaccgt tctcaatctc tataagctgt atctgatcgt
     2641 cgataaagag aatgaaaaag cgattcacat ttaccgcaag cttggcttta cggttgaagg
     2701 tgaattgatg catgagtttt ttattaatgg tcaatatcgt aatgccattc gcatgtgtat
     2761 attccagcat cagtatctgg cagagcacaa aacaccggat cagactctcc tgaagccgac
     2821 cgcacaatag cattaataat aatcgatcgt atttttgatg gtgtaaaccc gttcgacggc
     2881 gggttttact ccttcatcaa caatgatcag ctgacagtcc actggattgg cgtgactgtc
     2941 atattcacag ctctgtttta cattaccaac tcgttgatta tttagtaaag taaccgctgt
     3001 gtaatctaat tttttgatcg gatccgttga tggcgtggcg ctgacagata atgttttgtc
     3061 gttacttttc gtggttttac ccagcggata cccctgatca tcatagcgat attccatctg
     3121 catttgtttg ctgctggctt taatcacgaa gccattatca tcggtttccc aactcacccc
     3181 ggcagaaggt aattctgcta gctggcattt tccctgtaaa cgtactctct tctccagcgt
     3241 ctcggcatca cggtaataat tggcgtccag taccagagcg accacggtat tattttccag
     3301 atccagtaat tcgagtgaat caaaacagcc ttcttccgac aaagtcccag aaacacgttt
     3361 cgtcacttca ccttgctcat ccattaatgt ctgagtgaaa tcttttaccg gaccacgcag
     3421 cggatcaaaa tcgaattcat tagagaaact ggccatctca ggggtaaatg aaagtgttac
     3481 ctctgtgcgg tcacatcctg tgaggaatat cgcgagtaag catggtagta atttgtattt
     3541 cacaacagtc accagagagt agagatgatt ctcaatcata gtagcaaata cagtacttta
     3601 cacgttaaat gctatgctta aagaagttat cttcgcgtaa ggagcttatg atgaaactct
     3661 caacctgctg tgccgcgctt ctgctcgccc tcgcctcacc cgcggtactc gccgcgccgg
     3721 gatcttgtga gcgcatacag agcgatatat cacagcgcat tatcaataat ggcgtacccg
     3781 aaagcagctt cacgttaagt attgtaccca atgaccaggt tgatcagcct gattcccagg
     3841 tcgtcgggca ttgcgctaat gatacgcata aaattctcta caaccgcacc accagcggta
     3901 acgtctctgc tcccgcgcag tctacccagg atggcgcgcc tgccgaaccg cagtaacaca
     3961 ttatcgactg aacgccggat atgacacatc cggcgatttg aacatacaac ataatctcac
     4021 cttattactc atacccttct attgatgtgg attaataatt cttaacccaa aatgggtaga
     4081 ctccctctat tgttagcgcg ctaaatattc aatgtataaa catttatata acaataaaga
     4141 acagggagtg agttatgtcc aaaaatgacc gaatggtggg catcagccgc agaacactcg
     4201 ttaaatcaac cgcgacaggt tctctggcgc tggctgcagg cggtttttct ttaccgttca
     4261 ccctgcgcag tgcagcggca gcggtacaac aggccagcga aaaagtgatc tggggttcct
     4321 gttccgtcaa ctgtggtagc cgctgtgcac ttcgtctaca tgttaaagat aatgaagtga
     4381 cctgggtgga aactgacaat accggcagcg atgagtacgg caaccatcag ggtacgcgcc
     4441 tgtttgcgcg gtcgctccat ccgccggcgt attaatcatc ccgatcgctt gaatgaccca
     4501 atgaaacgcg tgggtaaacg cggtgaaggc aaattcgaac ggattagctg ggatgaagcc
     4561 ctggatacta tcgccagtag cctgaaaaaa accgtcgaac aatatggcaa tgaagctgta
     4621 tatattcagt actcttcggg gattgttggc ggcaatatga cccgctcttc gccatcagcc
     4681 tcggcggtca aacgcctgat gaactgctac ggcggttcac tcaatcagta tggctcctac
     4741 agcaccgcgc aaatttcctg tgccatgccc tacacctacg gcagtaatga tggcaacagc
     4801 accacggata ttgaaaacag caagctggtc gtgatgtttg gtaacaaccc ggcagaaacc
     4861 cgcatgagcg gtggtggcat cacttatctt cttgaaaaag cgcgcgagaa atcgaacgcc
     4921 aaaatgattg ttatcgatcc gcgatatacc gatacggctg ccggtcgtga agaggaatgg
     4981 ctccctattc gcccgggcac cgatgccgcg ctggttgcgg gtattgcctg ggtattgatt
     5041 aacgaaaatc tcgttgatca accttttctc gataaatact gcgtcggcta tgacgaaaaa
     5101 accttacccg cagatgcacc caaaaatggt cactataaag cctatattct tggtgaaggt
     5161 gacgataaaa cagcaaaaac gccgcagtgg gcttcgcaaa ttaccggtat cccggtagac
     5221 cgtatcatca aactggcgcg tgaaattggc acaacaaaac ccgcttatat ctgctagggc
     5281 tgggggccgc aacgccaggc aaacggcgag ctgactgcac gcgctattgc tatgttacct
     5341 attttgacgg gcaatgtcgg catcagcggc ggaaatagtg gcgcgcgtga atcgacctat
     5401 accattacga tagaacgcct accggtgctg gataatccgg tcaaaacgtc aatctcctgc
     5461 ttcagctgga cggatgctat cgatcatggc ccgcaaatga cggcactccg cgacggtgtc
     5521 cggggcaaag ataaactgga tgtgcccatt aagttcatct ggaacttcaa ggacttgaag
     5581 cggaccctta gtaggcctga ggtcctgcat tgagtcaaga ccttgtgtca ttctttgtgc
     5641 cagacttgtg tcattagacg ggatgtctag acggctggtc aggacccccg gtggcctcgg
     5701 tctggacttg aggtgggcat atgggggaag tctgctggct taatgtatta gatggtcatt
     5761 cacatttttc tgtcagaaac catcggtccc actgtgagtc caaacctaag gacctagaag
     5821 tccacccaca gccttagaca gagtgtcgaa cacatccaca ccgagtcgac cagagaggac
     5881 catgtagaga cccacagaga ggaacacgag tttatagacc agagtcagga gggaccttag
     5941 gtctgcggaa gtcatggaca taaagcctcc ttcgtctatt gtactactgc actagataac
     6001 tagtacaaaa caaaggtcca gaccattggt ccagaccata gttagttaat cacagtgata
     6061 gcccaccacc tgaagtcctg ctgatattca gtccagactt acctctgctc atggtcttac
     6121 ctccctcggt ctcctgacct tgggtatcga ctacagcttg ctgagcgtca tctggaggag
     6181 ctacaggagg agctacagga ggcgggaccg aagccccacc ttgagtccca ccaccacctg
     6241 tcaggccctt ggcgatggac ctgaagcctt tactaatgct cttacccatt ggtcttacgc
     6301 ttcggtcttg gtgaacttca ggcgtttgac tacaggcagg acggcagtag ctgagccagt
     6361 gcccatcttg acggtcgtag cagcttcagc gacaacgaac actttaccga ggatttccaa
     6421 gtcgtgggac tgagagtcgc catcacgtga aaggacgacc gtaatattct caccgtcgtg
     6481 acggaagcag ccgaagctca gggccgtgaa gcgtttagcc agcatgaaca cacctttgtc
     6541 ctcaccgttg taatacgcga tgtcggtacc cagcatatcc acgatagcgt cttcaccaga
     6601 gaagccctgt ttagcagcca gacgggacag cgctttcagt tgggactcat gggcgatgat
     6661 gccgtaatca gacacttcag agccgaaaga ggactcggtg ttctcaaaca gagcgtccat
     6721 gatagcgtct gccttagtgg tcaggcttcc agtcaggact ggcagctcta cggtcttcag
     6781 cagcttggag gactccagaa cgtcaaccat gcgagcgttg atggtacgga ccacattctc
     6841 acagatgttc atagcgtcct gctggagtgt gccatgagtg aaaccagtgg aggacttggc
     6901 gtcccacagt ttagtttgct ccgagatgag gattgcctgt tcgataggtt cgacgacaat
     6961 ctcctgacca gacggaacgg tcacgacttc aggtcgctca ccaccgcgta cagggacgac
     7021 cacagagcgg tcagacagag tgtgggcgat gattggggta ggcaggctat tgcgggtagg
     7081 caggacttct acagggaacg gaatgttatc ctgagcgtct ttaagtacac cgttcagcat
     7141 agccttaacc gtcttagcta actggagggc atagacctgt gggtcagcgt tagttggcag
     7201 accttcgtta acctgaatgt tgtcggtgtc ggtgatacgt actggctcaa tgccgaactg
     7261 atagctatct ttaatcatgg tcataataat caatctccta aattagtttt agatactgtg
     7321 ggtcccgaca tcaggtcagg accacttgtg tgtctgtgtc ttagaggctg tccatccagt
     7381 taccgcctga ctcaatgtca gcgacaatag cggcacggga gcgaccagcg gaatgggtag
     7441 cactgtggag gttctcacca taggtccggc gctcagcttt gacctgttcg cggcgctgta
     7501 gctcccgctg ccactctgcg tcggtcatgt tctcttcacg catacgctcg gcctgaagtc
     7561 gttgctcttt agcgatacgt tcagcctcag ctcgtttgtt catctggcgg cgttggtagg
     7621 cggtcatcag gtcattactg aagaagttgc caatagccac accagcttca gccagagctt
     7681 ccagtaattc catggactcg cggctggtgg caggcagtcc tgacaggtag cccggaatca
     7741 gggtcttaat agcgaaggac agtgggtagg actgaccgtc cagactcaca gtgttttcgt
     7801 agagattgat gtcggtgact gacagtttcc agtcgttgtt gttgaataac ataaattggt
     7861 actcctcttg agagttgaga ctatcggtat cgcagacgca gtagcgccta cgggtctccc
     7921 cgcatcggtc atctcaaaag gtgtttaaat taggtatggt atggtcagga ctgcttagca
     7981 aacgctcggc gtaactgatt attcaaatca cggtccacca gcatggctat cagctcgtct
     8041 cgactgtaga aaggacgctc ggggtcttcg actccaagtc tcagcagaga caggaggtga
     8101 cgtgtaggca gaccacggta acgaagggtc gctttgccga taggtgtgcg agggtcaatc
     8161 atagccatga ttacttaccc cactcacagc gaccagcggt cacagcttga tagtctgaat
     8221 actcagcctg tgagacttca cgtccggcga tgtagaggcc tgtgccatga cgctggatga
     8281 attccttggt ctccggcttg atgtcctcag cagacgctac agcagactgc acggtggcgc
     8341 tggaggtcat agacacgaag gcttcagaga ctgccagtcc gtactcaaag tccagccagc
     8401 gaccaaactc tacagtagcg gcggggtcag caaggacggt agacccgagt tcacccattc
     8461 ggataaggcg gattttctgc ctattcctca gtagctttcc aactttggtc tgagcaaaac
     8521 gcttgtactg tgtcgactcg gtgtcttcta ctttgccaat ctgaatgccg acccggcgaa
     8581 tttcatcgag tcgaaacaat ccgtcctgac tggtgatgtc ctgaccgaag atatgaatgg
     8641 tgtattcctg cttattgtca gaagtcataa gaggcacctc ctgccagagc ttcggcaaag
     8701 gacttgaagc gctgctcttg gtcagctttc gcctgaagac gttcggtatc aggtgggacc
     8761 agcttatcaa aggtgccgtt ttcgtaatcc tcagttagga cctgaagtcg gcgggaccat
     8821 ttgctctcca cctgcatcat cgcgttagag atgtgcttca agtcggcatc agagcgtgac
     8881 gctatccaat ccatctcttc tgggtcaccc tcacaggtgt aaggattgtt gaaatagaag
     8941 atgtcctgta ggccacgttt cagctcttcc tcggtgtgtg tctggaggta tgactggata
     9001 ggctccgcgt acatctcccg gattttctca tagtcccagt cttccgcttg ggcataccat
     9061 cgggataact tcagcaggtc taagttatag cgacagcaat cgacactgga ctctgcattg
     9121 gtcagcagga tgattaagaa ctggcggtta gttactggta attcggtatt catataaagg
     9181 atgctccttt ggtaaagcac tttctgtctc cacgtggagt ccgcacctat tcaatgcatc
     9241 caaacggacc caccaaaaag tgcttaacga aagtgaatag gattaaggat ggtctggagt
     9301 gacccggtgt ctctacatgt acgtataaag gcgagaccgc atgtcaggac gagctgtaag
     9361 gaccccagat ggtgagcggt gatagactta agccaatgta gtgagtagac gagctactaa
     9421 ctgagatgac tgaagaacgc taacgagctg gaggtcatac aggaggtctg acagaggcgg
     9481 tcacagacct tgactactta ggagagatac ttaatgtcgg ttgaccagat gctcgcatgt
     9541 tctgacgcat cagactcaga ggcaaggttg gtctccctat agtgatagtt taggggggtg
     9601 tccctatagt gagggggtat tccagcccag agctggcgcg gctctcagga caggcactta
     9661 tcgttgtagt cccaccatgt gaaatcccct cggtccttca gaaccttagc aagactcaca
     9721 gccgtagcct gcaagacgct attaacgtgg cgcaggtagc gaggcgaagg tgagcgaccc
     9781 gtgatggcgg tgtatgactt ggcagtgttg cgggagaaga tgtcgtttgt gccgtataag
     9841 gccgcgaaca ggtacgcctt ggataccggg agtccagcac cgatagagcc actggtagcc
     9901 cgtgagagtc ctgtgaacac atcgtcagcc cacgggtacg cctcaacaag ctgcgtatag
     9961 gagagacacc cagtctcaat cagccacgca cggacagccc ggttgtcgat gaccacagga
    10021 ggcttacc
//
LOCUS       DAEBXN010000136        10003 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.136, whole genome shotgun sequence.
ACCESSION   DAEBXN010000136 DAEBXN010000000
VERSION     DAEBXN010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10003
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.136"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(<1..191)
                     /locus_tag="KJL99_003636"
     CDS             complement(<1..191)
                     /locus_tag="KJL99_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBD4438219.1"
                     /translation="MSKPKYPFEKRLEVVNHYFTTDDGYRIISARFGVPRTQVRTWVA
                     LYEKHGEKGLIPKPKGVSA"
     gene            623..1741
                     /locus_tag="KJL99_003637"
     CDS             623..1741
                     /locus_tag="KJL99_003637"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HBD4438220.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTPPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FMALSAASPYMQGTDTRFAPSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            1807..2055
                     /locus_tag="KJL99_003638"
     CDS             1807..2055
                     /locus_tag="KJL99_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HBD4438221.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            2120..2488
                     /locus_tag="KJL99_003639"
     CDS             2120..2488
                     /locus_tag="KJL99_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HBD4438222.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYSGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2582..3235
                     /gene="nfsB"
                     /locus_tag="KJL99_003640"
     CDS             2582..3235
                     /gene="nfsB"
                     /locus_tag="KJL99_003640"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HBD4438223.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNKRKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFAYMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNTTLTEV"
     gene            3343..4590
                     /gene="ybdG"
                     /locus_tag="KJL99_003641"
     CDS             3343..4590
                     /gene="ybdG"
                     /locus_tag="KJL99_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HBD4438224.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4658..6034)
                     /gene="pheP"
                     /locus_tag="KJL99_003642"
     CDS             complement(4658..6034)
                     /gene="pheP"
                     /locus_tag="KJL99_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HBD4438225.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGYGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(6135..9278)
                     /gene="cusA"
                     /locus_tag="KJL99_003643"
     CDS             complement(6135..9278)
                     /gene="cusA"
                     /locus_tag="KJL99_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HBD4438226.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPKMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCAPFLWHVRSALVAIISLPLGLCIAFIVMPFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRN"
     gene            complement(9290..9808)
                     /gene="cusB"
                     /locus_tag="KJL99_003644"
                     /pseudo
     CDS             complement(9290..9808)
                     /gene="cusB"
                     /locus_tag="KJL99_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
BASE COUNT         2450 a         2758 c         2426 g         2369 t
ORIGIN      
        1 tcagcactaa cgcctttagg tttgggaatt aaaccttttt ctccatgttt ttcatagagg
       61 gcaacccatg tcctgacctg ggttcggggg acaccaaaac gtgccgagat gatcctgtaa
      121 ccatcatcag tagtgaagta gtgattcacg acttcaaggc gcttttcaaa agggtatttt
      181 ggctttgaca tattaggggc tattccattt catcgtccaa caaaatgggt gcagtacagg
      241 gttgatagaa atatctcctg gggcttcatc tttccagacc tcagagtaaa cctgaaatcg
      301 gaaagcttca ggcacccgca gttatcttac ccttcctcaa caaaatagct gtttatttat
      361 acagcaaatc ctgtttcgga cttctcactc cccatttgat caactgaagc tgaacccgtt
      421 tttttctgca tgcctcaatc gtcaaaattc ttcgccacca gccagctctg ccataacttg
      481 ccacattgaa aaagtagcca aagtgcttca ggcaccatca gttcaccatc accaaaaaaa
      541 agaaacattc cagaacttcc gcatctcgcc cagccgctcc gacttctata ctgaatagaa
      601 aacgtcaact taagagaaac ctatgccatt acccgatttt catgtttctg aaccttttac
      661 cctcggtatt gaactggaaa tgcaggtggt taatccgccg ggctatgact taagccagga
      721 ctcttcaatg ctgattgacg cggttaaaaa taagatcacg gccggagagg taaagcacga
      781 tatcaccgaa agtatgctgg agctggcgac ggatgtttgc cgtgatatca accaggctgc
      841 cgggcagttt tcagcgatgc agaaagtcgt attgcaggca gccgcagacc atcatctgga
      901 aatttgcggc ggtggcacgc ccccgtttca gaaatggcag cgtcaggagg tatgcgacaa
      961 cgaacgctat caacgaacgc tggaaaactt tggctatctc atccagcagg cgaccgtttt
     1021 tggtcagcat gtccatgttg gctgtgccag tggcgatgac gccatttatt tgctgcacgg
     1081 cttgtcacgg tttgtgccgc actttatggc cctttccgcc gcgtcgccat atatgcaggg
     1141 aacggatacg cgttttgccc cctcacgacc gaatattttt tccgcctttc ctgataatgg
     1201 cccgatgccg tgggtcagta actggcaaca atttgaagcc ctgtttcgct gtctgagtta
     1261 caccacgatg atcgacagca ttaaagatct gcactgggat attcgcccca gtcctcattt
     1321 tggcacggtg gaggttcggg tgatggatac cccgttaacc cttagccacg cggtaaatat
     1381 ggcgggatta attcaggcca ccgcccactg gttactgacg gaacgcccgt tcaaacatca
     1441 ggagaaagat tacctgctgt ataaattcaa ccgtttccag gcctgtcgct atggtctgga
     1501 aggcgtcatt accgatccgc acactggcga tcgtcgaccg ctaacggaag ataccttgcg
     1561 attgctggaa aaaatcgccc cttcagcaca taaaattggc gcatcgagcg caattgaagc
     1621 actgcatcgc caggtcgtca gcggtctgaa tgaagcgcag ctgatgcgcg atttcgtcgc
     1681 cgatggcggc tcgctgattg ggctggtgaa aaagcattgt gagatctggg ccggtgacta
     1741 agccgggaat tgccttacag aagaatatcc ctgacaatgg tgttttaact tacatttaac
     1801 aatgttatga aacacccttt agaaaccttg accaccgcag caggcatttt gctgatggct
     1861 ttcctctctt gcctgctgct gcccgccccc gcactggggc tgacgctggc acaaaaactg
     1921 gtgaccatgt tccatctgat ggatcttagc cagctttaca ctttattgtt ttgtctgtgg
     1981 tttttagtgc tgggcgctat tgagtatttt gttctgcgct ttatctggcg acgctggttc
     2041 tcgctggcgg attaaacgtc gattatcgcc gaacttcgcg gcatactttg cttattctcc
     2101 ttcgcctaaa ggaacgttta tggataagca atcactgcac gaaacggcga aacgcctagc
     2161 tcttgagtta ccctttgtcg agctttgctg gccttttggc ccggagttcg atgtttttaa
     2221 aattggcggc aagattttta tgctgtcgtc ggagctacgc ggcgtcccct ttatcaatct
     2281 gaagtccgat ccacaaaaat ccctgttaaa tcagcaaata tacccaagca ttaagccagg
     2341 gtatcacatg aataaaaagc actggatttc ggtgtattcc ggcgaggaaa tctccgaagc
     2401 gttacttcgc gatctgatca acgattcgtg gaatctggtg gttgatggtc tggctaaacg
     2461 cgatcaaaaa agagtgcgtc caggctaaag cgggaatcta tagcgcattt ttctcgctta
     2521 ccatttctcg ttgaaccttg taatctgctg gcacgcaaaa ttacttacac atggagtctt
     2581 tatggatatc atttctgtcg ccttaaagcg tcattccact aaggcatttg atgccagcaa
     2641 aaaacttacc ccggaacagg ccgagcagat caaaacgcta ctgcaataca gcccatccag
     2701 caccaactcc cagccgtggc attttattgt tgccagcacg gaagaaggta aagcgcgtgt
     2761 tgccaaatcc gctgccggta attatgtgtt caacaaacgt aaaatacttg atgcctcgca
     2821 cgtcgtggtg ttctgtgcaa aaaccgcgat ggacgatgcc tggctgaagc tggttgttga
     2881 ccaggaagat gccgatggcc gctttgccac gccggaagcg aaagccgcga acgataaagg
     2941 tcgcaagttc ttcgcctata tgcaccgtaa agatctgcat gatgatgcag agtggatggc
     3001 aaaacaggtt tatctcaacg tcggtaactt cctgctcggc gtggcggcgc tgggtctgga
     3061 cgcggtgccc atcgaaggtt ttgacgccgc aatcctcgat gcagaatttg gtctgaaaga
     3121 gaaaggctac accagtctgg tggttgttcc ggtaggtcat cacagcgttg aagattttaa
     3181 cgctacgctg ccgaaatctc gtctgccgca aaacaccacc ttaaccgaag tgtaattctc
     3241 tcttgccggg catctgcccg gctatttccc ctcagattct cctgatttgc ataaccctgt
     3301 ttcagccgtc atcataggct gctgttgtat aaaggagacg ttatgcagga tttaatatcc
     3361 caggttgaag atttagcggg tattgagatc gatcacacca cctcgatggt gatgattttc
     3421 ggtattattt ttctgaccgc cgtcgtggtg catattattt tgcattgggt ggtactgcgg
     3481 accttcgaaa aacgtgccat cgccagttca cggctttggt tgcaaatcat tacccagaat
     3541 aaactcttcc accgtttagc ttttaccctg caggggatta tcgtcaatat tcaggcggta
     3601 ttctggctgc aaaaaggcac cgaagcggca gatattctga ctacctgcgc gcagttgtgg
     3661 atcatgatgt atgcgctgct ttcagtcttc tcgttgctgg atgttatttt gaatctggcg
     3721 cagaaattcc cggcggcatc tcagttaccg ctgaaaggga tatttcaggg gattaaactg
     3781 atcggcgcaa tcctggtcgg cattttgatg atctcgctgc tgattggtca gtcgccagcg
     3841 attctaatca gcggtcttgg tgcaatggct gccgtgctga tgttggtatt taaagatccg
     3901 attcttggtc tggtggcagg tattcagctt tccgcgaacg atatgctgaa actgggcgac
     3961 tggctggaaa tgccgaaata cggcgcggat ggcgcggtga tcgatattgg gttaaccacc
     4021 gtcaaagtgc gtaactggga caataccatt accactattc ccacctggtc tctggtttct
     4081 gactccttta aaaactggag cgggatgtca gcatctggcg ggcgacgtat taagcgcagt
     4141 atcagtattg atgtcaccag tattcgtttt cttgatgaag acgaaatgca acgtctgaat
     4201 aaagcgcatt tgttaaagcc ttatttaacc agccgccatc aggaaattaa tgagtggaat
     4261 cgccagcaag gttctacgga gtcggtatta aacctgcgcc gaatgaccaa tattggaacc
     4321 tttcgtgcct atctgaacga atatctgcgt aaccatccgc ggattcgtaa agatatgacc
     4381 ttaatggtac gccaactggc tccaggtgat aacggtttac cgctcgagat ctacgcgttt
     4441 actaacaccg tggtgtggct ggaatatgaa agcattcagg ctgatatatt cgatcacata
     4501 tttgcgattg tcgaagagtt tggtctgcga cttcatcagt cgccaaccgg caatgatatt
     4561 cgctctctgg cgggtgcatt taagcagtaa ttaaaaaaac cgctctcatc gaatggatga
     4621 gagcggacat cgttttgtcg gatgtagcgt gaatgcctta tttccgacgc agcgttttaa
     4681 atgccacaaa caggaataca atccacaccg gcagcaggat cgctgacaag cgcatatcat
     4741 ccatcgtgca catcagcagc aaaatcatgc cgaggaaggc gatgcaaaga tagttgccga
     4801 acggatagag cagcgcctta aactgtgttt cacgcccctg acgtcgcatc gccgcgcgaa
     4861 aacgcagatg cgccaggcag atcataatcc agttcaacag caacgttgct accaccagcg
     4921 ccatcagcag accaaacgct ttttgcggca gcagatagtt gattaacacc accagcgaag
     4981 tgatcgctcc ggaaagcatc agcgagttaa tcggcacacc gcgacggctg acgcgagtca
     5041 aaaacttcgg cgcattaccc tgcacagaaa ggccaaacag catgcggctg ttagagtaaa
     5101 ccccgctgtt atacaccgac agcgatgcca ctagaatgac gaagttcagc gcagaagcta
     5161 ccacattgct gtcgagatta tggaaaatca tcacaaacgg gctactgttg gatttcactt
     5221 ccacccacgg atagagtgcc agtaaaacca ccagtgaacc gatataaaac agcaggatgc
     5281 gatacaccac ctgatttacc gcttttggaa tgcttttttc cggatcgcgc gcttcagcgg
     5341 ctgtaatccc aatcagctcc agaccgccga aggagaacat aattaccgcc agcgacaaaa
     5401 tcagcccatt ccagccggtg gcgaagaaac caccgtagcg ccagaggttg tcgatactgg
     5461 ctttctcgcc gccgtaacca gaaaacagca gccacaggcc aaagccgatc ataccgatga
     5521 tcgccagcac tttaatcagc gcaaaccaga actcggtttc gccatataag cgcacgttca
     5581 ccaggttaac ggcgttgatg ataataaaga aggcggcagc ccaaatccac gttggaacat
     5641 ccgggaacca gtactgcata tagatgcccg cagcggtcag ctctgccatt cccaccagca
     5701 cgaacattac ccagtagttc cagccagaga ggaagcccgc aaacggtccc cagtatttat
     5761 aggcaaagtg ggcaaatgaa ccggataccg gctcttcgac caccatctcg ccaagctggc
     5821 gcataatcag gaaagcgatg atcccggcga cgccgtagcc cagcaataca gccggacccg
     5881 ccatctgaat cgccgggcca atgccaagaa acagaccagt accaattgcg ccacccagcg
     5941 caatcagttg aatatgacgg ttatgtaatc cgcgatgaag cgtcggctct tgattcgacg
     6001 cagtatcttc cgatacggtt gacgcgtttt tcacgccttt cccctgtgtg tcttttttgt
     6061 tgaggggcac cttttaacat ttagtgccca tcgtcgcaag acacaatcca cacggttaaa
     6121 cgaggtatcc tgctttaatt ccgtacccga tgtcggtgca gccacatcag cttatacgcc
     6181 gccgggataa taaacagcga cagcaaaggt gcggtgatca tgccgccaat catcggtgcg
     6241 gcaatccggc tcatcacctc tgaaccagcc cccgttcccc acagaatcgg cagcagaccg
     6301 gcgataatca ccgccaccgt catcgctttc gggcgcacgc gcaggaccgc gccgtgatac
     6361 aacgcctcat ccagcttctg ctcgctgaat gtttgtggat tattcaacga cggctcggcc
     6421 tctatggcgt gacgtaaata catcagcatc accacgccaa attcggcggc gaccccggca
     6481 agggcaataa agccggtacc cgtcgccaca gaaagatgaa agcccatcca ccacagtaac
     6541 cagatgccgc ccaccagcgc aaacggtacg ctgctgataa tcagcaacgc ttcgcccacc
     6601 cgacggaatg ccagatacaa cagcacgaag atgatcatca gtgtcatcgg caccatgagt
     6661 ttcagcttat ggttggcgcg ctccagtagc tcgaactgcc cggagaatgc cacgctggtg
     6721 cccggtttta actgcacttt ttcagctatc gctttttgca aatcgtgaac caccgacacc
     6781 atgtcacgat cgcgggcatc gatataaatc cagctcgtcg ggcgcgcatt ctcggttttc
     6841 agcatcgacg gtccggtaga gactttaacg tcggccacgt ctgccagggt gatttgctgc
     6901 ttcatcggcg tcaggatcgg caactggcgc agtgcctgcg gactatcgcg ccagctttgc
     6961 ggataacgca gattaattgg ataacgggca atcccttcca ccgtttcgcc aaccatcgcc
     7021 ccgcccaccg cagaagtcac aaacaactgc acatccgcca ccgtcatacc gtaacgtgcg
     7081 gctttttcac ggttaatctc aacgttgata tagcgcccac cttccagccg ctcagcaagc
     7141 gcagaagcta cgcctggcac cgttcgcgcc acttcttcaa tttgctcagc catcgcgtcg
     7201 atatccgcca gcacagtgcc ggaaacttta atgccgatgg ggcttttaat gccggttgag
     7261 agcatatcga tacggttacg aattggcggc acccacagat tcgccagccc cggcagacgc
     7321 acggtgttat ccagttcctc aatgattttg tccattgtca tgcctggccg ccactgatcc
     7381 tgtggcttaa gctggatggt cgtttctacc atctccagcg gagcagaatc ggtggcggtt
     7441 tccgctttcc cggttttgcc aaatacccgc gccacttcag gtacgctcat aattagcttg
     7501 tcggtttttt gcagcatact cgccgcctct gctgcggaaa tccccggcag cgtcgatggc
     7561 atatacaaca agtcgccttc attgatctgc ggtaaaaatt ccccgccaac tttattgagc
     7621 ggccagagaa ccgtcagcac cgaaagcgcc gccaccagca gcgtggtttt cggccagtgc
     7681 agtactttca gcaacagcgg atgataaaca cgaatcaaaa agcgattgag cggattactg
     7741 ctttccggcg gaattttgcc acggatccag taacccatca ggatagggat cactacgatc
     7801 gccagcagcg ccgcacccgc catcgcatac gttttggtga acgccaacgg gccaaacaga
     7861 cgcccttcct gcccttccag ggtgaagatc gggataaacg acaacgtgat aatcagcaga
     7921 ctgataaaca gcgccggccc cacttcaaca gacgcatcgg tgatcacctg ccagcgcgtt
     7981 ttattatcca gcgtggcgtc aggatgctgg tgctgccact cttccagccg tttatgcgca
     8041 ttctcgatca tgacgatagc ggcatcgacc atcgccccga cggcaatcgc aatgccaccc
     8101 agcgacataa tattggcatt cagcccctgg aagggcatga caataaaagc aatacacaac
     8161 cccagcggca acgaaataat cgccaccagc gccgagcgca catgccagag aaacggcgcg
     8221 cagactaccg ccaccacaat aaactcttcc agcaacttgc cgctgaggtt gtcgatggcc
     8281 cggtcaatga gctggctgcg gtcgtatgtt gtaactatct ccacgccttc cggcagacta
     8341 cttttcagcg tttccagttt gtccttcacg gcggcgatca cttctcgggc gtttttgccg
     8401 gatcgcagga tcaccacccc gcccgccact tcgccttcgc cgtttagttc ggcaatgccc
     8461 cggcgcatct tcgggccaat ctggaccttc gcaacatcgc gcagataaac gggcacgcca
     8521 ttttcactgg cttttaaaac gatgtgatta aagtcgtcga gcgtttgtag atagccgctg
     8581 gcgcgcacca tatattctgc ttccgccagt tcgattgacg aaccgcccgc ttcctggttt
     8641 gaagcatcca gcgcgctttt tacttcggcg agactgatgc catactgcgc caggcgctga
     8701 ggatcgataa ccacctgata ctctttcacc acaccgccta ccgacgccac ttccgcaacg
     8761 tcaggaatgg ttttcagctc atatttgaga aaccagtcct gcaatgagcg taaatcggca
     8821 agatcgtgct taccgctgcg atccaccagt gcatattcat agatccagcc aacacccgtg
     8881 gcatctggtc ccagctcggc gctgactccc gcaggcagct taccctgtac ctggttgagg
     8941 tactccagca cccgcgaacg cgcccagtac ggatcggtgc catcttcgaa aatgacatac
     9001 acataagagt cgccaaactg cgagaaacca cgcacagtct tcgcgccagg caccgacaac
     9061 atggtggtgg ttagcggata agtcacctga ttttcaacga tttgcggtgc ctggccggga
     9121 tagctggttt taataatcac ctgcacatcg gagagatccg gcaacgcatc cactggcgta
     9181 ttaatgatgg tccaggtgcc ccagatgctc agaaacaacg cgcccatcag caccaaaaaa
     9241 cggttcgcca ccgagcgacg aataatccat tcaatcattg gttattccct caatgcgcat
     9301 gggtagcact ttcagagcgc atccgctcca gtgcgccaga aatattggct tcggaatcaa
     9361 tcaggaacag gccgccggaa accacctttt caccttccgc cagaccagag cgtaatgcgg
     9421 tgacgccttg cgatgcctgg aaaacagcaa cgcgtttcgg tacaaagcgc ccgtcggcat
     9481 caacggtaat cacccgctgt tcgttgccgg tatcaatcag cgcctgtgac ggaatgagca
     9541 gcatcggttc gctggcggtg ttgagttgca accaggcgtt catgcccggt tttagcgcct
     9601 cgtcggcgtt gtcgacttcc agacgcagct gcagcgtgcg ggtcgcggca tccacgccag
     9661 gtagcagcgt ccatttgcgg atggtgagtg ttttatccgg tcgcgccgga acggtgaggg
     9721 taaactgcga ggcatctttc accagccagg cgatggactc cgggatcgca gcagtgaccc
     9781 acaccgggtc cataccctga attcgcgatt acgcgcaatc cccctgagaa ccgccatgaa
     9841 caagggtttc atgatttgtg attttagttt ggtacgcaat ttggtacaca acactatttt
     9901 cacttcagcg ggtagtcatc aaactcacca taacgagcat cgttgatgat gtatgtgatc
     9961 accacaaata tcacatctgg actagttccg tcttcatgcg cag
//
LOCUS       DAEBXN010000137         9936 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.137, whole genome shotgun sequence.
ACCESSION   DAEBXN010000137 DAEBXN010000000
VERSION     DAEBXN010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9936
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.137"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(<1..1023)
                     /gene="bglB"
                     /locus_tag="KJL99_003645"
     CDS             complement(<1..1023)
                     /gene="bglB"
                     /locus_tag="KJL99_003645"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HBD4438227.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYYRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMLYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKAYHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVG"
     gene            complement(1042..2919)
                     /gene="bglF"
                     /locus_tag="KJL99_003646"
     CDS             complement(1042..2919)
                     /gene="bglF"
                     /locus_tag="KJL99_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HBD4438228.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFGFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADAPGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGQLSTWISEMIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHRGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTLMLH
                     FITSKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(3053..3874)
                     /gene="bglG"
                     /locus_tag="KJL99_003647"
     CDS             complement(3053..3874)
                     /gene="bglG"
                     /locus_tag="KJL99_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HBD4438229.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRLISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMHLVSAQ
                     MSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILEHASIN
                     DSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRKEH"
     gene            complement(4160..4885)
                     /gene="phoU"
                     /locus_tag="KJL99_003648"
     CDS             complement(4160..4885)
                     /gene="phoU"
                     /locus_tag="KJL99_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HBD4438230.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(4900..5673)
                     /gene="pstB"
                     /locus_tag="KJL99_003649"
     CDS             complement(4900..5673)
                     /gene="pstB"
                     /locus_tag="KJL99_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HBD4438231.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRVEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(5765..6655)
                     /gene="pstA"
                     /locus_tag="KJL99_003650"
     CDS             complement(5765..6655)
                     /gene="pstA"
                     /locus_tag="KJL99_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HBD4438232.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(6655..7614)
                     /gene="pstC"
                     /locus_tag="KJL99_003651"
     CDS             complement(6655..7614)
                     /gene="pstC"
                     /locus_tag="KJL99_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HBD4438233.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(7701..8741)
                     /gene="pstS"
                     /locus_tag="KJL99_003652"
     CDS             complement(7701..8741)
                     /gene="pstS"
                     /locus_tag="KJL99_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HBD4438234.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(8989..>9936)
                     /locus_tag="KJL99_003653"
     CDS             complement(8989..>9936)
                     /locus_tag="KJL99_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBD4438235.1"
                     /translation="SGYLITANENKVGTTFNTTVTNGSSYLGRCHCDTGNVGEFPYIY
                     YTSKINQALTYAGVHSNINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKY
                     NCNRIEPLSISSGAKAIVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMAD
                     VYIRGDITAPQSCEINNLKPVCFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLG
                     ILNTDDISTSFYATEPNTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMDKSTV
                     YGEKSGSVTFSAAPASLTGARPAPGQFTATATITVEIVR"
BASE COUNT         2563 a         2586 c         2294 g         2493 t
ORIGIN      
        1 tccaaccgga tcaatttgcc acccccactc tgaacttttc agatgcggat tggggatcat
       61 attcagtatg ttgccctgcg catttttatt aatgctttcg tcgtgggaaa cacaaccagt
      121 catgtaataa ctaaaagaga tgaaatctac agtatgtttt aaatcttctg cgtcactttc
      181 agtcatctca atggtgatat tgtggtcgcg gaagaaacgc tgcatatagc cgggatattg
      241 gccacgcgcc tgaacatcac caaagaacat ccagcgccgg ttctcttcca tggcctgcaa
      301 catatcctgt ggctggcagg tgagggggta aaccagccca ccgagaagca tattgccgat
      361 tttcgcttcg gggatcaggc tatgataggc tttaactgcc cgcgcactgg caaccagttg
      421 atgatggatc gcctgataaa cttccgcctc gccactctct tctgccagcc ccacgcccgt
      481 gaatggcgcg tgtaacgaca tattgatttc attaaacgtc agccataacg ctactttatg
      541 ttggtagcgg gtaaagaccg tgcgggcgta atgctcgaag tgatcgatga ccgcccgatt
      601 agcccaaccg ccgtagtttt tcaccagccc atatagcatt tcgtaatggg ataacgttac
      661 cagcggcttg atccccgcct gcgccatttc atcaaacagc cgatcgtaaa acgctaaccc
      721 cgcttcattc ggttcggctt cgtcgccctg agggaaaatt cgcgcccagg caatggaaat
      781 acgcagacag gtgaagccca tctcggcaaa taacgcgata tcttccgggt aacggtaata
      841 aaaatcgatg gcgacatctt tgatattctc tttccccagg atgcgcggtt ccatttttcc
      901 cattacgcca tgaggctgta aatctgaggt cgtgatccct ttgccatctt ccagccaggc
      961 accttccacc tgattggcag ctgttgcgcc accccaaaga aatgtttctg gaaatgcttt
     1021 cataattaac tccttttatc gttagcgaat gatggataac agcggttcac ctgcgcttat
     1081 ctgcgccgtg ccgtggggta atacgtctgt aaaatcatcg ctattactga ttaataccgg
     1141 cgtcgtcaga tcaaatccgg cctcgcgaat agcagggata tcaaaagaaa tcagccgatc
     1201 gcctgtattg accttgtcac ccacgttgac gtgagcggaa aagaatttgc cgtccagttt
     1261 tacggtgtcg ataccgacat gaatcaggat ctccacacca tcatctgact caatgccaat
     1321 ggcgtgtaat gtggcgaaca acgaagcaat tcgacccgca accggagaac gcacttcacc
     1381 aaccgagggc agaatggcga tacctttacc caacaggcca ctggcaaacg tggtatcagc
     1441 gacgtgaatg agcggcacaa tctctcccgt catcggtgaa cagataccgc cctgctcagg
     1501 tggtgtaata acctctggtg ttttctcttg cggggcaccc tgcgctggct gacgtttact
     1561 ggtgatgaaa tgaagcatca acgtaccaac aaatgcgcaa cctatggcaa tgacaccgcc
     1621 aataacgctg gcccagacgg tgaaatcaat ccccgttgac gggatggtct gcatgaaggt
     1681 gaaaatactt ggcaaaccaa aggagtaaac tttcgtttgc gcgtagccaa tgatggtggc
     1741 ccccaatgct ccactgatac aggcgataac aaaggggtac ttacgcggca ggttgacgcc
     1801 atataccgct ggttcggtga taccaaacag acccgtcaac gccgctgatc ccgccaccac
     1861 ttttttctgc gcatcgcgtt cgcagaggaa gacgccgagc gccgccccga cctgcgccat
     1921 aatggcgggc attaacagcg ggatcatggt gtcgtagccc agcacggtga agttattgat
     1981 acacagcggc accaggcccc tgtgcagtcc gaacatgacg aagatttgcc agaagccgcc
     2041 cattaccgcg cccgcaaatg caggaaccgc ctgataaagc cagagataac cggcggcaat
     2101 catttcgctt atccaggttg atagctgccc caccagcaga aaggtgacgg gtgtgataac
     2161 catcagacat agcaatggtg tgaagaaatt tttgattgcc gacggtaacc acgcattaag
     2221 tcggcgttcc agaatgctgc acaaccaggc agaaaaaata atgggaataa ccgatgacga
     2281 gtaattcaac aatgtgaccg gaatacccag gaaatccagc cccggcgcat ccgctttttg
     2341 cccgttctcg aaggcagtca gaattaatgg atgcactaac gctccaccaa tcaccatggc
     2401 agtaaatgga ttaccgccga agcgtttgcc cgcggtgtat cccaggatta tcgggaagaa
     2461 cccaaacaag gcatcactgg cgctgaataa aattaaataa gtaccacttt gttcggtcgt
     2521 ccactgaaaa gtgagcgcca gagccagcat acctttcagg atcccggttg ccgccatcag
     2581 accgatcaga ggcgtaaaaa tacctgaaat aacataaaca aagcggttta gcagattacc
     2641 tttctcatca ttttccggtg cctgttgcgc tttttcgcca aggcctgcca cgctgttaac
     2701 cgccaggaag acatcggcca catggttacc tatgaccacc tgaaactgac caccgctttc
     2761 caccaccata ataataccgg gggtcttttt cagtacctca gcttgcgctt tgctttcatc
     2821 ctttaactta aaacgtaatc gcgttgcgca atgcatcaga ctcacaatgt tatctgcgcc
     2881 cccgactcct gcgactattt ttctggctaa ctccgtcata acttgccctc taccgctttg
     2941 cggcaaaact ccaaaaaaaa acctgaaaaa aacggcctga cgtgaatcaa gcaatttttt
     3001 tcaggttttg cccgcttagt gcggtaacaa tcctttactc agtaataata tttcagtgtt
     3061 ctttgcgcac gcgctctata tttatggcta aaaacataat ctctgcgggt gaaattttac
     3121 gttgatactg caaaccaata aaaatggcga tccgttccgc acattgccat gcttgcgggt
     3181 aattttgttt tactgcttgt tgtaatgatt catcactatc gttaattgaa gcatgttcaa
     3241 gaatacgcca ggataaaaac ttcagatgtg taaccagtcg ctgataactc aagctttctt
     3301 cctggtaatt aaggctgaac tgaaatttta ttaattgcag catttcgcgc attaactgcg
     3361 tgacacctgc aacatcctcc atatttccgc tcatttgggc actgaccaga tgcatggcaa
     3421 taaagcccac ttcatctttc ggtaactgca cgcccaaccg tttatcaata atggttaacg
     3481 cttcttcccc tagctggaac tctttcgggt aaagccgctg gatatcccac agcaacacgt
     3541 tttgctgaaa gcgtttaatc gcaaactggc aatggtcagt tagcgagata taaatactgt
     3601 cctgtaattt tcccaagcgc tcctgcgcta aagagataag acgatcacag gttgccatca
     3661 cctcaagagg aatatgactt aagagttcgc ttaatcgccc gttcagttca tgactgctca
     3721 aggcatactc tttttctatt ccacttgagt taattctttc gccagcgcgt ttttgaaagc
     3781 caattccgcg ccccatgacg actttttccc gctgttgatc atcaataacc accacaacat
     3841 tattgttgag aattttggtg atttgcatgt tcatagcaag gacctttttt ataaacaaaa
     3901 aaaaccgact tcaccagtat tctctggtta tgtcaggttt tgcctgcgaa tgcagtaaca
     3961 atccagtcat ttattaatgg tttttataac gaacatccag gttcggaaat taatttaatt
     4021 gcgtgcagag aaaatagcaa tgcgctattg ataaaaatat gaccatgctc gcagttatta
     4081 actttgtgta attttaggaa tttataaagt tatatataac aaatcccaat aattaagtta
     4141 ttgggatttg tctggtgaat tatttgtcgc tatcttttcc cgccagcagt ttatccagct
     4201 catcaccgcc tacatgacgg aaatcctgac ccttcacgta gtagaagata aactcacaaa
     4261 tattctggca gcggtcgccg atacgttcga ttgaacgcgc acagaacagc gcggtaagta
     4321 cgctcggaat agtgcgcgaa tcttccatca tgtaggtcat cagttgacga acaatacctt
     4381 cgtattcctg atcgactttt ttatcttcac gataaatacg taccgcttcg tcaatgtcca
     4441 tccgcgcgaa cgcgtccagc acgtcgtgca gcatctggat ggtatgacgg cccagcgact
     4501 ccagacttac cagcaacggc tgatgctgct gggagaattt ctccagcgca gtacggcaga
     4561 ttttgtccgc cacgtcgcca atacgctcca gctcggcaat ggttttactg atcaccataa
     4621 ccagtcgcag gtcgctcgcc gtcggctgac gtttggcgat aatgcgcacg caggcttcat
     4681 cgatcgccac ttccatcatg ttgacgttct tgtcgccttc gatcacgcgc ttcgccagat
     4741 cgctgtcctg gttatgcatc gcggtgattg catcagaaag ctgctgctcc accatgccgc
     4801 ccatggtcat cacctgcgtg cggatacttt ccagttcggc gttgaactgg ccggaaatat
     4861 gtttattaag attgagactg tccataacgc actcctgaat caaccgtaac gaccggtgat
     4921 gtagtcttct gtttgtttct tcgctggctt ggtgaacaga tcgtccgtgt tgctgaactc
     4981 aatcaattcg cccaggtaca taaatgccgt gtggtcggaa caacgcgcag cctgctgcat
     5041 gttgtgggtg acgatcacca cggtgtaatc ctgcttcagt tcggtgatca gctcttcaat
     5101 acgcccggta gagatggggt cgagcgccga acacggttcg tcgagcagca gcacttccgg
     5161 gcgaatggcg ataccacgcg caatacacag acgctgttgc tgaccaccag agagagagta
     5221 accgctctgg tgcaatttat ctttggtttc gttccacaat gcggctttgg tcaatgccca
     5281 ctgcacgcgc tcgtccatgt cggcacggga gagcttctca aacagacgaa cgccaaaggc
     5341 gatgttgtcg tagatggaca tcgggaacgg cgtcggtttc tggaacacca tgcccacttt
     5401 cgcacgcagc agggcgatat cctgagagtt ggtcaggatg ttgtcgccat caagcagaat
     5461 ttcaccttcc acacgctgct ccgggtacag ttcaaacatt ttgttgaagg tacgcagcag
     5521 cgtcgattta ccgcagccgg acggcccgat aaacgccgta acctggtttt tggcgatatc
     5581 caggttgatg tttttcaggg catggaattt gccgtagtag aagttcaaat tacgaacctg
     5641 aattttactc ggggcagttt caaccatact cattgcaatc ttttcctcat cgtacctgat
     5701 gcttttcgct aatcaggctt accgtagggc cgggtaaggc gttcacgccg catccggaaa
     5761 agtgttaacc gtgtttattc ttcgcaaaaa caacgcgcgc cagaatgttc agcagcagga
     5821 cgcacagggt aatgatcaat accccggccc aggccagttg ttgccattcc gcaaacgggc
     5881 tcatcgcaaa cttaaagatc gtcaccggca ggttggcgat cggctgcatc atgtccgtgc
     5941 tccagaactg gttggagagc gcggtaaaca gcagcggcgc ggtttcaccg gcaatacggg
     6001 caatcgccag caggataccg gtcataatcc cggacaccga cgctttcagc gtaatcgcgg
     6061 agatcatctt ccacttcggt gtacccagcg cataagccgc ttcacgcagg ctgtccggca
     6121 ccagtttcag catgttctca gtggtgcgga taacgatcgg cacctgcaac agcgccagcg
     6181 caattacgcc cgcccagccg gagaagtgct ccatctgtgc caccacgatg gtgtagacaa
     6241 acagaccaac cacaatcgac ggcgcggaga gcagaatgtc gttaatgaag cgaatcactt
     6301 ctgccagcca ggatttacga ccatattccg ccagataaat ccccgccata atgcccagcg
     6361 gcgtaccgaa taccgtggcc cacaaaatta acagcccgct acccgccagg gcgttcgcca
     6421 gaccaccacc ttccgtattg ggcggcggcg tcatttcagt gaacagcgcc agcgacatac
     6481 cgtcgatacc gcgagtgatg gtggacatta aaatccagat cagccagaac aggccgaagg
     6541 ccatcgtcgc catcgagagc gtcagcgcaa tacggttttt gaggcggcga cgcgcctgca
     6601 ttttgcggcg agattcagcc agcgccgcag tggtttgcat ttcaaccata gccattagcg
     6661 tgccccctca ttcttagcca ggcgcataat cataaacttc gatgcggcga ggacgatgaa
     6721 ggtaatcaca aacaggatca ggcccagttc catcagtgcg gcaacgtgca gaccggattc
     6781 cgcttccgca aattcgttcg ccagcgcaga ggtgatacta ttgcccggca tgtacagcga
     6841 ggcgctgtcg agctggtagg tgttaccgat gataaaggtc accgccatgg tttcaccgag
     6901 cgcgcggccc agccccagca tgatgccgcc gataacacca tttttggtga acggaagaac
     6961 gatacgccag ataacttccc aggtggtgca gccaataccg taggccgact ctttcatcat
     7021 caccggggtt tgttcgaaca catcacgcat taccgccgca atgtacggaa taatcatgat
     7081 ggcgaggatc acgcctgccg cgaggatacc gataccaaat gcggggccag agaacagcgc
     7141 gccaacaatc gggatattcg acatgatatt gccgaccggc tcctgaaagt aaacggcgaa
     7201 cagcggcgca aagataaaca ggccccacat gccgtaaacg atacttggaa tggctgccag
     7261 cagctcaatg gcgataccca gcgggcgttt cagccagcca ggcgcaagct cagtcaggaa
     7321 cagggcgata ccgaaactca ccgggacggc gatcagcagc gcgataaacg aagtcaccaa
     7381 cgtaccgtag atcggcacca gcgccccgta gatatcgttc ggtgcatccc actctttggt
     7441 ccataggaaa gccagaccaa atttctgaat gctcggccag gaggagatga tcagagagac
     7501 aataatgcca cccaacatca atagcacaat cagcgccgcc agttttacca gcacgctgaa
     7561 aattatgtcg ccctttttac ccggtgggtt aaaagcgggc ttggttgcag ccataagtta
     7621 ctcttcagtt aaacgcgttt acaaagtcat tttgtaatgc cggatgcggc gtaaaacacc
     7681 gtacccggcc tggagtttta ttagtacagc ggcttaccgc tactgtcttt aatattggtc
     7741 ttccacgcag cgcgaacctg ttcaactaca ctatccggca ggctggcgta atccaggtcg
     7801 ttcgcctgtt tagccccggt tttgtacgcc cagtcgaaga atttcagcac ctctgtgcct
     7861 tgttctggtt tcttctgatc tttgtggatc agaatgaacg tggtagaggt aataggccat
     7921 gcatcttcgc ctttctggtt ggtcagatcc tgagcgaagg ttttgctcca gtctgcacct
     7981 tttgctgcat tagcgaagtt ttcttcggtc ggactaaccg gtttaccatc agcggagatc
     8041 agtttggtgt acgccaggtt gttctgcttc gcgtaagcat attcaacata accaattgca
     8101 cccggcagac gctgaacgaa cgcggcgata ccgtcgttac ctttaccgcc cagaccgatc
     8161 ggccatttta cggtagagcc agtaccaacg ttgtttttcc actcttcgtt cactttcgcc
     8221 aggtagctgg tgaagacgaa ggaagtcccg gagccatctg cgcggcgtac tacagcaatg
     8281 ttttgtgaag gcagtttcag acccggattc agtttggcga tggcttcatc atcccacttc
     8341 ttgattttgc ccaggtagat gtcgccgagg gttttaccat ccagcaccag ttcgccagac
     8401 ttcagccctg gaatgttaac cgccagcacc acgccgccaa tcacggtcgg gaactggaac
     8461 agaccttcct gagccagttt ttcgtcagac agcggcgcgt cagaggcacc aaaatcaacg
     8521 gtattagcga taatctgttt tacgccaccg gaagaaccga taccctggta gttaacttta
     8581 ttaccggttt ctttctggta agtgtcagcc catttggcat acaccggcgc agggaaggtt
     8641 gcacctgcac ctgtcaggct tgcttctgca aacacagaga aagcactcat cgataaggtc
     8701 gcggcgacaa cagttgcgac ggtggtacgc ataactttca taatgtctcc tgggaggatt
     8761 cataaagcat tgtttgttgg ctacgagaag caaaatagga caaacaggtg acagttatat
     8821 ataaggaata tgacattttt atgacagaaa ggaattacca aataacttac tgattttaaa
     8881 tgtgaagatg aaaattccac atgccatctt tttgtcatga tgcatttaaa agtgataaag
     8941 aaatgggtga gatgacaacg atgtgatgaa caatcgttgt cagacctatt agcgcacaat
     9001 ctcaacagtt atcgtcgctg ttgcagtaaa ttgccccggg gctgggcgag ctccagtaag
     9061 gctcgcagga gcagctgaaa aagttacact acctgatttt tcaccataca ctgttgattt
     9121 atccatgtct gttggcaact caccaccgtt cactttgatt tccttattat ttttgtcgta
     9181 aatttttatc cccacgttag agtttgaagt tacgaccatt gaattgtccg tattgggttc
     9241 ggtagcataa aaagacgtac tgatatcatc ggtattcagt atccccaggt tttcacactc
     9301 aatagtgact gttttagtaa ttttatgtgt tgttaccgca cttccaacaa cagatgaaaa
     9361 atctgcagct ggtatttctt taaaatcaaa acaaactggc tttaaattat ttatttcgca
     9421 actttgcgga gctgtaatat cgccccgaat ataaacatca gccatcggct gtgagtaatc
     9481 caccggggtg tcacggctaa ttgttccata caatgtcact atctttgttt caggaataat
     9541 caattttcct gcaaatgtct tcttgatata aaaatatact atcgctttag ccccactgga
     9601 tatacttaaa ggttcaatgc ggttgcaatt gtactttgta ttaccagacg ggttgttagc
     9661 atgatattca aaaggtgcat tcacataccc aaccccaaga atatctatag caatcccaac
     9721 atcaaggttc ggattaagat cataataatt aatattagag tgaaccccag cataagttaa
     9781 tgcctggttt atttttgacg tataatagat ataggggaat tctcctacgt taccagtatc
     9841 acaatgacag cgtccaagat atgaggatcc attagttaca gtggtgttaa atgtcgttcc
     9901 aaccttattt tcgttcgcgg taataagata tcccga
//
LOCUS       DAEBXN010000138         9930 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.138, whole genome shotgun sequence.
ACCESSION   DAEBXN010000138 DAEBXN010000000
VERSION     DAEBXN010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9930
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.138"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..341
                     /locus_tag="KJL99_003654"
     CDS             <1..341
                     /locus_tag="KJL99_003654"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438236.1"
                     /translation="LSSNNEYDLFKNRETSLWKRKVPESAALAMTGTVYKLFGNRLLN
                     ARNQLDKNLLEMAMQMVYGKDMVTTSGDILIDIQLNKDGSLQSKHYTLDVGEVINSYN
                     IDPLCECFKY"
     gene            511..1644
                     /locus_tag="KJL99_003655"
     CDS             511..1644
                     /locus_tag="KJL99_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005052835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438237.1"
                     /translation="MKGGISMFNHTTKTADNIEHNLQLSFLVGSRPSITNKYSAKDVG
                     PDILALPVNVYHADFKIKINNAVEKASSIVLCAVQKNLHAARKVLSGIIGNVPKNNKS
                     KTIIFDLRFLSAIKQKVFLGSEKPLFVKHTAMMREACKELPQSVEYVPLACEAHSHRS
                     VALAKTVAAPIMATGVGLALMYATSSGPYYAQSLSGSPDIEMIKNRMAELFNQSDISV
                     RNKYSPAFDKWNELVDKLNHERNTVPFACLTTILATAINETLEGDTNVAVVMGCKSAK
                     DRTISIVLGNSMLQTLLEKRLADSGEIENLFDQQGYFSCDSLTPEELMMLKDLFDIRV
                     LHVSNKFNVGLQGNINTDVLQDSFFKNVDFIHEYNSYTVGMGV"
     gene            2323..2541
                     /locus_tag="KJL99_003656"
     CDS             2323..2541
                     /locus_tag="KJL99_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HBD4438238.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            2759..3001
                     /locus_tag="KJL99_003657"
     CDS             2759..3001
                     /locus_tag="KJL99_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438239.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(3331..4260)
                     /gene="fdhE"
                     /locus_tag="KJL99_003658"
     CDS             complement(3331..4260)
                     /gene="fdhE"
                     /locus_tag="KJL99_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HBD4438240.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQKVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(4257..4892)
                     /gene="fdoI"
                     /locus_tag="KJL99_003659"
     CDS             complement(4257..4892)
                     /gene="fdoI"
                     /locus_tag="KJL99_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HBD4438241.1"
                     /translation="MKRRDTIVRYTALERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHQNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(4889..5791)
                     /gene="fdoH"
                     /locus_tag="KJL99_003660"
     CDS             complement(4889..5791)
                     /gene="fdoH"
                     /locus_tag="KJL99_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HBD4438242.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(5804..8854)
                     /gene="fdnG"
                     /locus_tag="KJL99_003661"
     CDS             complement(5804..8854)
                     /gene="fdnG"
                     /locus_tag="KJL99_003661"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(8267..8269),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HBD4438243.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNHEYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            9048..9881
                     /gene="fdhD"
                     /locus_tag="KJL99_003662"
     CDS             9048..9881
                     /gene="fdhD"
                     /locus_tag="KJL99_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HBD4438244.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGRLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         2489 a         2396 c         2543 g         2502 t
ORIGIN      
        1 gtttgtcgag taataatgaa tatgacctgt ttaaaaatag agaaacctca ctgtggaaac
       61 gcaaagtacc agaaagcgca gcgctggcaa tgacgggaac agtatataaa ttatttggta
      121 atcgcttact caacgcccgc aatcagttag ataaaaatct cctcgaaatg gctatgcaaa
      181 tggtttatgg caaagatatg gtgacaacgt ccggggatat attgattgat atacaattaa
      241 ataaggatgg cagtctccag agtaagcact acacacttga tgtaggtgaa gttataaaca
      301 gttataatat tgaccctctt tgtgaatgct tcaagtatta atcaaactta cttacatgat
      361 aaaaatcctg gtgacgttat tagcctgtat ggcactttag ataacattcc ccttgtagtt
      421 aaggaatgca cgggtaaaaa tcatcaaatt aatgttaaag ccaaattgcc acaagaacga
      481 agtgaaaaag ttgaggatat gtgtgaaaga atgaaaggtg gcatctctat gtttaaccac
      541 accacaaaaa cagctgataa tatagagcat aatttgcagc taagttttct cgtgggttca
      601 cgtccgtcga tcaccaataa atactcagca aaagatgtag gtccagatat acttgcattg
      661 cccgtcaacg tatatcatgc ggattttaaa attaaaatca ataatgcagt ggagaaggct
      721 tcatcaattg tgttatgtgc tgtgcaaaaa aatctccatg cagcaaggaa ggttcttagc
      781 gggataatag gaaatgtgcc aaaaaataat aaatcaaaaa caattatttt tgatttgcgt
      841 tttcttagtg caattaagca gaaagttttt ttgggcagtg aaaaaccgtt gtttgtaaag
      901 cataccgcaa tgatgaggga ggcatgtaag gaattaccgc agtcagtgga atatgttcct
      961 ttagcctgtg aagcgcatag ccatcgcagc gtcgcccttg ccaaaaccgt ggctgcgccc
     1021 atcatggcga caggtgtggg cttggcatta atgtatgcaa catcatcagg accgtattat
     1081 gcgcaaagtc tttctggttc gccggatatc gagatgataa aaaatcgcat ggctgaattg
     1141 tttaaccagt cggatatttc tgtgcgcaac aaatatagcc cggcttttga taaatggaat
     1201 gaactggtag ataaattaaa tcacgaaaga aatacagttc cgtttgcgtg tttgaccacc
     1261 attttagcga cggcgataaa tgaaactctt gagggggata cgaatgttgc cgttgtgatg
     1321 gggtgtaaat ctgcgaaaga tcgcacgata tccattgtcc ttggcaactc gatgttgcaa
     1381 acactccttg aaaaacgcct cgccgatagt ggagaaatag aaaatctttt tgatcaacag
     1441 ggatatttta gttgcgattc attaacaccc gaagaattaa tgatgttaaa agaccttttt
     1501 gatattcgag ttcttcatgt ctctaacaaa ttcaatgttg gattgcaagg taatatcaac
     1561 actgatgttt tacaagattc gttttttaaa aatgtcgatt tcattcatga atataactcg
     1621 tataccgtag ggatgggagt ctgacgagta aaactcatta ggattatcag aattcggaca
     1681 atgcattgca aaaatgacat acaaagagag atgtgtgcag gtacttagcc gcgaggggca
     1741 tggcgaagcc atacagaaag taccagtatt cagaactcca gtgaaagcgg catagtggcc
     1801 gcctgctcag gagttttgct atgacccgta atcgtgctga acgtcgccac cctatgatgc
     1861 gattaaaaaa actcatcgtc gcgctgatga ttgacgtcaa tgctatcgga tgatgccggg
     1921 ggtgttccgg aaggaattcc ttgtttcccg aactgttacc aacaaaatta taacgatgag
     1981 caagtaaaga ccggttggtg gcagtactga ccggtctgtg atgaaagatt aatttcacct
     2041 taaatcctgg tgctgttagg atgctgacgt ttttcggcta caccattcag attatgtaaa
     2101 taaatatccc tgaatataat atattcatag cacccataac tcatcctggg cgataactaa
     2161 accatttttg agtaataaat gaaacaactg caacgctctt attatttctg gaaaacgcct
     2221 cgcaaaaaca aaatgacatg caaaaatccg cattttttta aatgcttttc cggcgtaatt
     2281 ttcgcacata cattaatgag taatatatgt aaggtggtgg taatggcgat gaatacggtt
     2341 tttcttcatt tatcagaaga ggcaattaaa cggctgaaca agcttcgggg ctggcgtaag
     2401 gtttcacgat ctgcaatttt acgcgaagcg gttgagcaat atctggagcg gcagcaattt
     2461 ccggtgcgta aagcaaaggg aggcaggcaa aagggggagg tagttggtgt tgatgatcag
     2521 tgtaaggagc ataaggaatg aaaaaaattt ttcattcttg aatataaaaa acagatgccc
     2581 ttattctggt attaatacaa ggctgtttta cttgaactta taataactgc aactgttaca
     2641 tcgtatctgg aaaacgcctc gcaaaaataa aaaatgatgc gtaaatgagc tttctttaaa
     2701 gattcggcaa gggtactttt ctctccatag attcaaaaat gatattggcg agatatttat
     2761 gaactctctg gcaggtatag atatgggcag aattttactc gatttatcga atgaggtgat
     2821 taagcaactt gatgatctcg aggtgcagcg taatcttcct cgtgcggacc tattaaggga
     2881 agcggtagat caatacctga taaatcaatc gcaaacagca agaaccagtg ttcctggcat
     2941 ctggcaaggg tgtgaggaag atggtgtcga atatcagcgt aagctgcgcg aggaatggta
     3001 atgggaaaaa atgatgtcaa tcagattgct gacaacgtgc gcgttgttca tgccggatgc
     3061 ggcgtgaacg ccttatccgg cctacaaagt catgcaaatt caatatattg aagtttattg
     3121 gtaggcctga taagcgcagc gcatcaggca atcatacgct tgtcatcagt gacataccca
     3181 taaccttatc gatgtgttgg agggaagaac gaggcaaaca gacaattgcg gcatgatctt
     3241 gaatgggtaa cgcggaatac gagagatggt gtcgtcacac cctatgagct gtaatccggg
     3301 cgacctgcgc cacccggaaa acgtcagaga ttactctcct tcacccggaa acaggaacgg
     3361 gttaatggaa ctacgggcgt agccttcttg ctccattcgc gcgtccagta ccagagaggc
     3421 gaggtcatct gccacggctt caactttcgg ttctttttcc tgataaagaa tcttcaggta
     3481 agtgccgcag tcatcgcagc tttcggcttt aatcgcggcc tgttcgtcat ccagcgacca
     3541 gtaatgcagt ttgccgctct gctcgcagtt gctgcatttt acgcgcacta cgtgccattc
     3601 ggtttcacac aggttgcagt gcaggtaacg cagaccctga gtggtgccaa tttgcaccat
     3661 gctggacacc ggcatagagc cacataccgg gcaatattga cgttgttcgc cgtattcagc
     3721 gcgggctttg ccggggatca gattggccat ctgcgcccag tagagcgaca gcgcagccca
     3781 gataaacggc gctttatcgc tgctgacgga cgagaaatca gaggcaaaca gcgcgctggc
     3841 catatcttcc agctcctgag tcgatgcctt ctccagattc tcaatcactg ccagcgccgg
     3901 gccgctcatt tcaggtttca gctcagcaat tagcgccatc agcagctttt gccagtgctt
     3961 atcacgcggc agaacgtgaa tatccagcgg gggcttgccc tgtgcgctgg cttctttaat
     4021 gcgcgcggtc agatccatct ccagcggatg gtcgtacagc accactttct gggcgtgggc
     4081 gataagcgca gcaaagcgca ggtaatcacc cagcggatta ttttctgcca gctcgcgcag
     4141 acgctcggcg cggcggttgt ataaattctt gagccgaggg aacaataacg gcggaatcat
     4201 atccgccgta cgtttctcgc tcgaacccag ctcatcttgc gggattatgc gaatactcat
     4261 tcagctttct tttccgttgt cttgcggacc tcacggtacc agcgcgggtg atgtttcttc
     4321 gcccaggcgc tggtaaccca tccttccacc atggcggtaa tcgtgccttt cacccaaagg
     4381 gcggcgtaga tatgcaccat gataaccaca attaacgcca ctgcggcaaa tgaatgcagc
     4441 attaacgcga atcggatcac cgggattgag aaagcaggcg caaaataagg acgccagata
     4501 atcacgccgc tcaccagcag cagaaccagg aaaataatcg ccgcccagaa aacgcatttc
     4561 tgaccgaagt tataacgccc ggtgtcacct acttcctcgt tgacgacgat cttacgaata
     4621 ttcttcgccc aaaagatatc atcccgattg attaggtttt gatgccagta acggaaaaac
     4681 atgatgatga acgaggcaaa cataaccacg ccgacaaacg ggtgcagaat tcgcgccagc
     4741 tgcggtgtgc ccatgatttg catcaaccag ttgaaggacg ggaacaaaaa gcccagcccg
     4801 ctcaccgccg ccaggatgaa gcagaaggcg gtgatccagt ggttgatacg ttccagcgcg
     4861 gtgtagcgca cgatggtgtc acgtcgtttc atttgcgctc ctcgtctttc tcttcgtgca
     4921 gattattctc ttcctcatcc gcacggttcg gaccgacacc cacgtagtgg aagatactgg
     4981 ccgcaaaggt agccgcaaag ccaacagccg cgagcggttt ccagatgcct ttccagaatt
     5041 tcacggtttc gctgatttcc gggttctccg gcaagccatg atacagattt ggcttgtcag
     5101 catggtgcag cacgtacatg acgtgtgtac caccgacgcc tgccggatcg tacagacccg
     5161 cattgtcgta accgcgggtt ttcagctcag caacgcgctc gctcgccagc gttttcatcg
     5221 actctttcgt gccgaagtga atcgcgcccg ttgggcaggt cttcacgcag gctggttctt
     5281 gcccaaccac cacgcggtca acgcacagcg tacatttgta gacgcggttg tcttccgggt
     5341 tgaggcgcgg aatgtcgaac ggacagcccg caatgcaata accgcagcca atgcactgct
     5401 cggactggaa gtcgacgata ccgttggcat actgaataat tgcaccttcc gccgggcacg
     5461 ctttcaggca gcctggatcg gaacagtgca tacagccgtc tttgcggatc agccattcca
     5521 gtttgtcgtt ctgctccact tccgagaagc gcattaccgt ccacgatttg gcgcttaaat
     5581 cattggggtt gtcgtacacc ccaatgttat tgccgacggt atcgcgaatg tcgttccact
     5641 ctgaacacgc cacctgacag gctttacagc cgatacaggt ggtaacgtcg atgagtttcg
     5701 ccacttcttc ctggaagtcc cgcgcctgag gcgcgggggt cagaccgtta gtcgcggaac
     5761 gacgaatgat atcttgagat tgataagcca tatgtcgtct ccgttacacc ttttccacat
     5821 tcacaaggaa ggacttaaac tccggcgtct gcgtgttcgc atcaccaacg aatggcgtta
     5881 aagtattggc aataaagcct tttttagcta cgccttcata gccccagtga atcggaatac
     5941 cgatggtatc gatatctttg ccgttcgctt tcagcgtgcg aatacgtttg gtcaccaccg
     6001 ctttggcttt gatatagcca cggttggagg agactttcac ggtatcgccc tgggcaatgc
     6061 caagtttatt cgccagcgac tccccgattt ccacaaactg ctctggctgc aaaatcgcgt
     6121 tcaacagcgc gtgtttggtc cagtagtgga agtgctcggt cagacgatag gtggttccga
     6181 catacgggaa cttatcggct ttacccaatg cttcggcgtc gtctttaaag atacgtgcag
     6241 ctgggttcga gataacgttt ggatgcagcg ggttagttcc cagcggcgtt tcaaacggct
     6301 cgtagtgttc cgggaacgga ccttccgcca tcttatcgag ggcaaacaga cgccccatgc
     6361 cttcctgctg catgataaac ggcccgacgc cgctacccgg aggcgctgcg ctgtagtccg
     6421 gaatatccca gccggtccac ttagtgccgt cccatttcag caactgacgc ttcggatccc
     6481 acgggttacc ctgcggatct gcggaggcgc ggttatacag aatgcggcgg ttaagcggcc
     6541 atgcccatgc ccagcccagc gtgttaccga ggccagacgg atcggcgtta tcacgacgcg
     6601 ccatctggtt gccttccggc gtccagctac cggcgaaaat ccagcagcca caggaggttg
     6661 taccgtcatc gcgcaattgg gcgaacgagc taagttgttg gcctttcttg acgataaccg
     6721 caccggttgc cggatcggta atatcggcca gcgccttacc gttgctctcc atcgctactt
     6781 cttccgactt cggctcatgc ggaatggcgt agttccaggt catgttcagc acctgatccg
     6841 ggttcgcgcc accctgttcg gcatacatct tgcgcaagcg caggaagata ccggagagga
     6901 tctcaccgtc agtcagcgca atccccgggg cgtccgcacc tttccagtgc cactgcaacc
     6961 agcgacctga gttaacgatt gaaccgttct cttctgcgaa gcaggtcgat ggcagacgga
     7021 acacttcggt ctggatcttc gacgagtcaa cttcgttcag ctcaccgtgg ttctgccaga
     7081 agttagaggt ttcagtgttc agtgggtcga tggtgacgag gaacttcagt ttcgacagac
     7141 aaccgatcac tttgtttttg ttcgggaatg aggcaacagg gttaaagccc tggcagatat
     7201 agccattgac cttgccctct ttcatcatct cgaagtattg caggacgtcg tagcctttat
     7261 cccacttcgg caaccagtca aagccccagc tattttccgc cgtcgcttta tcaccaaaga
     7321 aggccttcat catagagacg aagaatttcg ggtagttgcc ccagtagttt acctggcctt
     7381 ccagcagcgg ttttggcgtg ttggcggtaa ggtaggtttg cagatcggtc tgcttctcgc
     7441 ttggcagcgt catgtaacct ggcagactct gcgacagtag ccccaggtcc gtcagcccct
     7501 gaatattgga gtgaccgcgc agggcgttaa cgccgccgcc ggccatcccc atattgccga
     7561 gcagcagctg gatcatcgcc atcgtacgaa tgttttgcgc accaacggag tgttgcgtcc
     7621 agccgagggc atacaggaac gaggcagttt tatcgtgagc actggtttct gcgatgtatt
     7681 cgcagacttt caggaacgcg tcttttggcg taccacagat gttttcaacc acatctggcg
     7741 tgtagcggga aacgtgctgt ttcagcaagt tccacacgca gcgcggatgt tgcagcgtgg
     7801 tatcgcgttt ggcgaagccg ttttcgtcca gttcataagt ccaggaggat ttatcgtact
     7861 tgcgtttttc cgcgtcgtag ccggtgaaca ggccatcttc aaagccgtaa tcctcacgca
     7921 cgatcaggct ggcgttggta taggcttcgg tgtattcgtg gttgaatttt tcattgttca
     7981 gcaggtacag caatacgcct gacaggaaag caatgtcagt accggaacga ataggggcat
     8041 agtagtcagc taccgccgcc gtacgcgtaa agcgaggatc gatcacaatc agcttcgcgc
     8101 cgttgtgaat tttggcttcc atcgcccagc ggaacccgac cgggtgagct tcagcggcgt
     8161 taccgcccat caccacgacg aggttggcgt tcttgatgtc gacccagtgg ttggtcatcg
     8221 caccgcgacc aaatgttgga gcaagacttg ctaccgttgg tccgtgtcag acacgcgcct
     8281 ggttgtcgac cgcgagcata cccagcgcgc gggagaattt ttgcgttaaa tagccggttt
     8341 cgttactcga cgcggaagca cacagcatcc cggtggagag ccagcggtta acagtcacgc
     8401 cttcggcgtt ttgcgcaatg tagttagcat cgcggtcttc tttcatcagt ttggcgatgc
     8461 gatcaaacgc ctcttcccaa ctgatttgct gccatttatc agaacctggc gcacggtatt
     8521 ccggaaactt cagacggctt tcggagtgga tgaaatccac caggccagcg cctttcggac
     8581 aaagtgcacc gcggttgacc gggtgatccg gatcgccttc gatatggaag atagatgctt
     8641 tggcgttttt tgctccgtca ccgaggctgt acatcaacag cccacagcct acggaacaat
     8701 aggtgcaggt attacgggtt tcgcgggtgc gcagcagttt atactgccgg gtttccgcga
     8761 gtgctacgct gggtgcaaaa cccagtgccg ctgccgtggt gcctgccata ccgccagcgc
     8821 agatcttaaa gaactgcctt ctgctgacct gcatggattg ctccttgttt cgacattgtc
     8881 acgtcccatt tacattcgct tgctgcgtgt gcagggagtg ggagttattt ttctttgcgg
     8941 aaggggccgc aaaggtccag aattggctca atttccctcc atctaggaag ggtttgtaac
     9001 agaataccat aatgttggtg tgtatgttct tatctggtta agagaaagtg aaaaaaacac
     9061 agcgaaaaga aatcgaaaat gtgacaaata tcacaggtgt tcgtcaaatt gagttatggc
     9121 ggcgtgatga tttacaacac ccacggttag atgaggtcgc ggaagaagtt cccgttgcgc
     9181 tggtctacaa cggcatttcg catgtggtga tgatggcgtc gcccaaagac cttgagtact
     9241 ttgcgctcgg tttttcgctt tccgagggga taatagaaag tccgcgcgat atcttcggca
     9301 tggatgtcgt tccttcctgt aatggtcttg aagtacaaat tgagctttcc agccgccgct
     9361 ttatggggtt gaaggagcgc cgccgggcgc tggcgggacg tacgggctgt ggcgtatgcg
     9421 gcgtggagca acttaatgac atcggaaaac cggtgcagcc gctaccgttc acccagacgt
     9481 ttgatctcaa caaactggat gatgcattac gtcatctcaa cgattttcag ccagtggggc
     9541 gactgactgg ttgtactcac gccgctgcct ggatgttgcc atctggcgaa ctggtcggcg
     9601 ggcatgaaga cgtgggtcgc catgtggcgc tggacaaact gttaggccgc cggtcacaag
     9661 aaggggaaag ctggcagcaa ggtgcggtac tggtttccag tcgtgccagt tatgaaatgg
     9721 tgcaaaagtc ggcgatgtgc ggcgtagaga ttttgtttgc ggtgtctgcc gcgaccacgc
     9781 ttgctgtaga agtggctgag cgctgtaatc tgacactggt aggtttttgt aaaccgggtc
     9841 gggcaacggt ttatacccat ccgcagcgtt tgagcaatta attaaaataa gtcctgcgat
     9901 atatattaaa ttgcaggact tattcatttc
//
LOCUS       DAEBXN010000139         9905 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.139, whole genome shotgun sequence.
ACCESSION   DAEBXN010000139 DAEBXN010000000
VERSION     DAEBXN010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9905
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.139"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(160..900)
                     /gene="pflA"
                     /locus_tag="KJL99_003663"
     CDS             complement(160..900)
                     /gene="pflA"
                     /locus_tag="KJL99_003663"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HBD4438245.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(1092..3374)
                     /gene="pflB"
                     /locus_tag="KJL99_003664"
     CDS             complement(1092..3374)
                     /gene="pflB"
                     /locus_tag="KJL99_003664"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBD4438246.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLSYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(3429..4286)
                     /gene="focA"
                     /locus_tag="KJL99_003665"
     CDS             complement(3429..4286)
                     /gene="focA"
                     /locus_tag="KJL99_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HBD4438247.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWCQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            complement(4692..6452)
                     /locus_tag="KJL99_003666"
     CDS             complement(4692..6452)
                     /locus_tag="KJL99_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HBD4438248.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLETEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            6582..7208
                     /locus_tag="KJL99_003667"
                     /pseudo
     CDS             6582..7208
                     /locus_tag="KJL99_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            7407..8495
                     /gene="serC"
                     /locus_tag="KJL99_003668"
     CDS             7407..8495
                     /gene="serC"
                     /locus_tag="KJL99_003668"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HBD4438249.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            8566..9849
                     /gene="aroA"
                     /locus_tag="KJL99_003669"
     CDS             8566..9849
                     /gene="aroA"
                     /locus_tag="KJL99_003669"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBD4438250.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGLSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLDSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
BASE COUNT         2520 a         2368 c         2483 g         2534 t
ORIGIN      
        1 ttcgatgggg aagaacttca cgcgagctat tatcagtcgt tattatctca ggtacgggat
       61 atggtagatg cactataaga tgtgttaaaa acgctgtagc agaatgaagc gcggaataaa
      121 aaagcggcaa ctcaataaag ttgccgcttt acgaggaaat tagaacatta ccttatgacc
      181 gtactgctca agaatgcctt tcacgcgttc catggtctct ttcttcggtg gtttaacacc
      241 atcgagtttg tattcttcac ccatcgccac ccatttgtgt ttgcccagtt cgtggtaggg
      301 gagaagttcg attttctcaa cgttacccat atcacgggta aattcaccga ggcgatgtgc
      361 cgaatcgtca tcgtcagacc agcctgggac aacaacgtag cggatccaca ccttcacatt
      421 tttgttcgcc agatatttag cgaactccag cgtgcggtgg ttggaaactc caaccagatt
      481 ttggtggatc tcgtcgttca tctgtttgag atcgagcatt accaggtcgg ttacttccag
      541 cagttcatca atcaccggat cgtaacgacg aacaaaaccg ttggtatcca gacaggtatg
      601 aatgccttct tttttacagg cgcggaacca gtcacgaaca aactcagctt gcaggattgc
      661 ttcaccgccg gatgcggtaa cgccgccgcc ggaagcgttc ataaagtggc gataggtcac
      721 cacttccttc atcaaatctt caacggtaac ttctttaccg ccatgcgtgt cccaggtgtc
      781 gcggttatga caatacaggc agcgcatcag gcagccctgg aaaaaggtga taaagcgaat
      841 acctgggccg tctacggttc cacaggattc aaaggagtga atgcgaccaa taactgacat
      901 tgcggtgttt ctccagatgt ggcccatctg aggccgtgtt ggtgcgcagc tcgaaggcta
      961 cgtcgagtct gttttggcag tcaccttaaa gtatagatag ctgacaaaaa aggctctcgt
     1021 gctaaaaaag gccccacttt cgtggagcct ttattgtacg ctttttactg tacgatttca
     1081 gtcaaatcta attacataga ttgagtgaag gtacgagtaa taacgtcctg ctgctgttct
     1141 ttagtcagcg agttgaaacg tactgcgtag ccagatacac ggatggtcag ctgcggatat
     1201 ttttccgggt tttccatcgc gtcgagcagc atttcacggt tcatcacgtt aacgttcagg
     1261 tgctgaccac cttcgatgga tgcttcgtgg tggaagtaac catccatcag accagccagg
     1321 ttggtcttac gaacttcgtc gtctttaccc agtgcgttcg gaacgataga gaaggtgtag
     1381 gagataccat ctttagcgta agcaaacggc agtttagcaa cggaagtcag agacgctaca
     1441 gcacctttct ggtcacgacc gtgcatcggg ttagcacccg gtccgaacgg cgcgccagca
     1501 cgacgaccgt ctggggtgtt accagttttc ttaccataca caacgttaga agtgatggtc
     1561 agaacagact gagtcgggat agcgtcacgg taggtgtgca gtttctgaat tttcttcatg
     1621 aaacgttcta ccaggtcaac agccaggtca tctacacgcg gatcattgtt accaaactgc
     1681 gggtattcgc cttcgatttc gaagtcgata gccagaccgt cttcgtcacg aatcggttta
     1741 actttcgcat atttgattgc agacagggag tcagcagcaa cggacagacc agcgatacca
     1801 cacgccatgg tgcggataac gtcacggtcg tgcagcgcca tcagagaggc ttcgtagctg
     1861 tacttgtcgt gcatgtagtg gatgatgttc agtgcagtga tgtactgttt agccagccag
     1921 tccatgaagt gatccatgcg ctccatcact tcatcgtagc tcaggagatc gcctttgatc
     1981 ggttcagact tcggaccaac ctgcattttc agtttttcgt caacgccgcc gttgattgcg
     2041 tacagcatgg ttttcgccag gtttgcacgc gcaccgaaga actgcatttg tttaccaacg
     2101 atcatcgggc ttacgcagca agcgatagcg tagtcatcgt tgttgaagtc cggacgcatc
     2161 aggtcatcgt tctcatactg cagagaagag gtgtcgatgg acactttagc ggcgaatttc
     2221 ttgaagttca gcggcagttt ttcagaccac agaatggtca tgttcggttc cggagacggc
     2281 cccatagtgt acagggtgtt caggaaacgg aagctgtttt tggtaaccag ggtacgacca
     2341 tcgaggccca taccaccgat agattcggtt gcccagattg ggtcgccaga gaacagttca
     2401 tcgtattccg gagtacgcag gaagcgaacc atacgcagtt tcatgaccag gtggtcaacc
     2461 atttcctgcg cttcttgttc ggtgatcttg ccagctttca ggtcacgttc gatgtacaca
     2521 tccaggaagg tggaggtacg accgaaggac attgcagcac cgttctgaga cttaacagca
     2581 gccaggtagc cgaagtaagt ccactggata gcttcctgag cgttggtagc cggaccagag
     2641 atgtcgtagc cgtatttcgc agccatttct ttcatctgac ccagagcgcg gtgctgttca
     2701 gcgatttctt cgcgcagacg gatagtctgt tccaggttta cgccgttttc cagatcagcc
     2761 tgtagagagg tgaactgagc gtatttgtct ttcatcaggt agtcgatacc gtacagcgca
     2821 acgcgacggt agtcaccgat gatacggcca cggccataag catctggcag accggtcaga
     2881 acaccggatt tacggcaacg caggatgtcc ggagtgtaaa cgtcgaacac gccctggttg
     2941 tgagttttac ggtattcagt gaagattttt ttgatcatcg ggtccagttc gcggttgtac
     3001 gctttgcagg aaccttcgat cattttgata ccaccgaacg ggataagagc acgtttcagc
     3061 ggagcttcag tctgcagacc aacaactttt tccaacgctt tattgatgta gccagcgtcg
     3121 tgagaggtga tggtggaagc aacagcggtg tcaaaatcaa ctggcgcgtg agtgcggttt
     3181 tccagtttaa caccttccat tactttgtcc cacagggtgg tggtcgcttc agtagcgcca
     3241 gccaggaagg actcgtcacc ctcgtacgga gtgtagtttt tctgaatgaa gtcacggacg
     3301 tttacttcat tctgccagtc acctttggta aaaccttccc aggctgtggc taacttttca
     3361 ttaagctcgg acatgtaaca cctaccttct taagtggatt ttttatttac tgcgtacttc
     3421 gacaaccatt aatggtgatc gttttcacgc aggtaaatga cccagtatgt caacccaacc
     3481 aacaaaccac cgccgataat attaccgatc gtaaccggga tcaggttatc agtgatgaag
     3541 ttcatcacgg tcaggtgaga aaaattttcc ggtgcagaac cgacagcggt ccagaattcc
     3601 ggagatgcga agtcgcggat tacaataccc atcgggatca taaacatgtt tgcgatactg
     3661 tgctcaaaac cgctggcaac aaacatcgcg accggcagca ccataatgaa cgctttgtcc
     3721 atcaggctgc ggccagaata actcatccat actgccagac ataccatcag gtttgccagg
     3781 ataccaagac agacggcctc aataaaagta tggtgcactt tgtggtcggc ggtttgtagg
     3841 acgtttagtc cccattgacc atttgcggtc atatactcgc cggaaagcca cattaaaagt
     3901 acaaacagca gtgcgccgac caggttgcca aaatagacat ttagccagtt tttagccaac
     3961 tgacaccagg tgatgcgccc actcgcctta gcaacaacaa tcaacactgt ggaagtaaag
     4021 agatcggctc cgcagacaac acaaagaatc agccccagag agaagcaaat gccgccaacc
     4081 agttttgcca tgccgaaggg cattgtgcct gtgccagtgg ttgctgtgat atagaagacg
     4141 aatgcgattg agatgaaaac accggcggta atcgccagat agaaagtctt aagcggatgt
     4201 ttcgttgctt tatagacacc cgcctcttcg gccactttgg ccattgcagc aggaagtaaa
     4261 agatcaaaag ggttgtcagc tttcacacta actctctctt tattaagtcg gcgacgagat
     4321 actaacaaag cattatagat gagaaattga tatagatcat atctcgcctg gcttataggc
     4381 ccgtaactcg catggttttt atgcaaatac ggagtaacta tttgattatc caaataaaaa
     4441 taaattttaa aaattaacaa atgagttgaa ttttttccgc atcctccgct aaaacagtta
     4501 attaaaaggg agcatcaggc gaataaagta acaatatcga tcgtatttat taaatacaaa
     4561 ttaccgatat ttaaccttat aattacaatt attttattaa tgcaaatata tgtaaagcgg
     4621 ggcattaaaa aaacgccccg taatataact cagactaatc attaagccta cattgcgtag
     4681 gctatttgat tttattttgc ccagaatgct gctttggcgc gctgcagctt ttcgtaggcc
     4741 ttcaacaacg actgatgtgc agcaaacgcg tgcagatcgc tatctaccgg ttgcaggccg
     4801 taaaacgccg cttcgccgct cattgccgca ctggcggctt ctacggcatc tgcgccgtac
     4861 atgcgaacaa acgcattcag atattgcagc ggctggcgat cttcttcctg tgccagtaat
     4921 aacaacgttt gcaggcagcg ataatagttg gcgcgttccg gactaaatac tgatgagtta
     4981 aactccatcg tccattcggt ccagaccaga gcctgttcca gatcgccacc agccagcgcc
     5041 agcatggctt ttaattcacc gatacgcagg gtgtaccaac cgttatccga cccggtcgcc
     5101 agacccaaca gctcacgcac gcgggtaaag tcatcaaaac cttcttcatc cagttgctcg
     5161 atgaggttca ggtaatcttc tttttcccac tcgctgcctg gtagcgaaag aatcgtttca
     5221 cgtaaatggc tgcccatact gttattcgcg agccacagat cttcagccgg ataaatatcg
     5281 gacatgccag gcacgataat acggcaagca taaacgccca gatgctcgta atcggcaata
     5341 taaacttctt tatcttcttt gttgaagata gccatcagcg tagcaaactc ttcttccgtg
     5401 gtgccggaga aattccagtc cacaaacgga taatcggcat cctgcttgaa caggtcccag
     5461 gagattaaac cgctggaatc gataaagtgc gtttcgaggt tggtatgttc agcgacttct
     5521 tcatcatcga aggttggcgg agtaaacaca tccaaatctt tcaggccacg accttgcagc
     5581 agctcggtca cggtacgttc cagtgctacg ccaaaatcag gatgcgcacc gaaagaggca
     5641 aagcaggtgc cgtttgcagg attaaacagt accacgcaaa tcaccggata ctggccgcca
     5701 agggaaccat catatgcgaa gatcgggaaa ccttctgttt ccagtgtttc gatcgcttca
     5761 accaccgctg ggtaacgcgc cagcacgtct gccgggattt ccggcaagct gatgctttca
     5821 gcaataatgc ggtttttcac gtagcgttcg aaaacttcgg acaacccctg aacgcgtgct
     5881 tcgttgcggg tattgccagc ggacataccg ttagaaacgt acaggttacc aatgatattc
     5941 atcggaatat aaacggtctg attgtcggac tggcgcgtaa acggcagacc gcaaatacca
     6001 cgatcttcgt taccggattg taggtcaatc agcatactgc cggtcaattc attctccgga
     6061 tcgtaaaatg cgcgcagacg gtcatcgagc agcccttctg gcacatcgtc attttcgctc
     6121 agtgggaacc atttttcgtt gggataatgc acaaatggac cgttggcgat ggtttcgccc
     6181 agccagaaat cagcaaaaaa gtagttggtt gagagacgct cgaaatattc accgagtgca
     6241 gaagccagcg ccgctttctt ggttgcgcct ttaccgttgg taaaacacag tgcgcactct
     6301 ttgtcgcgaa tatgtacaga ccagacgtta ggcacgggat tcagccagga ggcctcttca
     6361 atctgaaagc cgaggtctga aagtttttgc tggaagcgag cgatggaatc ttccagagcg
     6421 gcatctttgc cggggataaa tgtttgcgtc atgaaaatca ctttagtcgt acggaaagcg
     6481 cgcaataata cgggttttat ctcaaaggcg ctatcaccgc cgccatgccg atgaactgtt
     6541 gactatgctt ttagcggata aaccacgtta agagcataaa aatgaaggcg ttcgatctcc
     6601 accgtatggc atttgataaa gtgccttttg atttccttgg cgaagttgca ctacgtagtc
     6661 tttatacctt tgtactggtt ttttgttcct caaaatgacc ggaagacgcg gtgtgcgaca
     6721 gatgtcgctg tttgaagtgt taatcattct cacgctgggt tcagcggcgg gagatgtggc
     6781 gttttatgat gatgtgccga tggtgccggt acttatcgtc tttatcactc tggcgttgtt
     6841 ataccgcctg gtaatgtgat ggcgagctgg cctggtcgaa actcaataac tccaacatga
     6901 cggaatttga gttctttatg gagctacgat tgcgtggcgt ggagcagctg gggcaggtac
     6961 gtctggcgat tctcgaaacc aacgggcaaa tcagtgtcta tttctttgaa gatgacaagg
     7021 tgaaaccggg tttacttatt ttacccagtg attgtacgca gcgttacaaa gtggtgccgg
     7081 agtcggcgga ctatgcctgc attcgttgta gtgaaatcat tcatatgaac gcgggggaaa
     7141 aacaattatg tccgcgctgt gcaaatccag aatggacgaa ggcaagtcgg gcaaaacggg
     7201 tgacctgaca gtaaaaacat cggctttttg ctaataatcc gagagattct tttgtgtgat
     7261 gcaagccaca tttttgccct caacggtttt actcattgcg atgtgtgtca ctgaatgata
     7321 aaaccgatag ccacaggaat aatgtattac ctgtggtcgc aatcgattga ccgcgggtta
     7381 atagcaacgc aacgtggtga ggggaaatgg ctcaaatctt caattttagt tctggtccgg
     7441 caatgctacc ggcagaggtg cttaaacagg ctcaacagga actgcgcgac tggaacggtc
     7501 ttggtacgtc ggtgatggaa gtgagtcacc gtggcaaaga gttcattcag gttgcagagg
     7561 aagccgagaa ggattttcgc gatcttctta atgtcccctc caactacaag gtattattct
     7621 gccatggcgg tggtcgcggt cagtttgctg cggtaccgct gaatattctc ggtgataaaa
     7681 ccaccgcaga ttatgttgat gccggttact gggcggcaag tgccattaaa gaagcgaaaa
     7741 aatactgcac gcctaatgtc tttgacgcca aagtgactgt tgatggtctg cgcgcggtta
     7801 agccaatgcg tgaatggcaa ctctctgata atgcggctta tatgcattac tgcccgaatg
     7861 aaaccatcga cggtatcgcc atcgacgaga cgccagactt cggcaaagat gtggtggtcg
     7921 ccgccgactt ctcttcaacc attctttccc gtccgattga cgtcagccgt tatggtgtaa
     7981 tttacgctgg cgcgcagaaa aatatcggcc cggctggcct gacaatcgtc atcgttcgtg
     8041 aagatttgct gggcaaagcg aatatcgcgt gtccgtcgat tctggattat tccatcctca
     8101 acgataacgg ctccatgttt aacacgccgc cgacatttgc ctggtatcta tctggtctgg
     8161 tctttaaatg gctgaaagcg aacggcggtg tagctgaaat ggataaaatc aatcagcaaa
     8221 aagcagaact gctatatggg gtgattgata acagcgattt ctaccgcaat gacgtggcga
     8281 aagctaaccg ttcgcggatg aacgtgccgt tccagttggc ggacagtgcg cttgacaaat
     8341 tgttccttga agagtctttt gctgcaggcc ttcatgcact gaaaggacac cgtgtggtcg
     8401 gcggaatgcg cgcgtctatt tataatgcca tgccgctgga aggcgttaaa gcgctgacag
     8461 acttcatggt tgagttcgaa cgccgtcacg gttaatgccg aaattttgct taatccccac
     8521 agccagcctg tggggttttt atttctgttg tagagagttg agttcatgga atccctgacg
     8581 ttacaaccca tcgctcgtgt cgatggcact attaatctgc ccggttccaa gagcgtttct
     8641 aaccgcgctt tattgcttgc ggcattagca cacggcaaaa cagtattaac caatctgctg
     8701 gatagcgacg acgtgcgcca tatgctgaat gcattaacag cgttagggtt aagctatacg
     8761 ctttcagccg atcgtacgcg ttgcgaaatt atcggtaacg gcggtccatt acacgcagaa
     8821 ggtgccctgg agttgttcct cggtaacgcc ggaacggcaa tgcgtccgct ggcggcagct
     8881 ctttgtctgg atagcaatga tattgtgctg accggtgagc cgcgtatgaa agagcgcccg
     8941 attggtcatc tggtggatgc gctgcgcctg ggcggggcga agatcactta cctggaacaa
     9001 gaaaattatc cgccgttgcg tttacagggc ggctttactg gcggcaacgt tgacgttgat
     9061 ggctccgttt ccagccaatt cctcaccgca ctgttaatga ctgcgcctct tgcgccggaa
     9121 gatacggtga ttcgtattaa aggcgatctg gtttctaaac cttatatcga catcacactc
     9181 aatctgatga agacgtttgg tgttgaaatt gaaaatcagc actatcaaca atttgtcgta
     9241 aaaggcgggc agtcttatca gtctccgggt acttatttgg tcgaaggcga tgcatcttcg
     9301 gcttcttact tcctggcagc agcagcaatc aaaggcggca ctgtaaaagt gaccggtatt
     9361 ggacgtaaca gtatgcaggg tgatattcgc tttgctgatg tgctggaaaa aatgggcgcg
     9421 accatttgct ggggcgatga ttatatttcc tgcacgcgtg gtgaactgaa cgctattgat
     9481 atggatatga accatattcc tgatgcggcg atgaccattg ccacggcggc gttatttgca
     9541 aaaggcacca ccacgctgcg caatatctat aactggcgtg ttaaagagac cgatcgcctg
     9601 tttgcgatgg caacagaact gcgtaaagtc ggcgcggaag tggaagaggg gcacgattac
     9661 attcgtatca ctcctccgga aaaactgaac tttgccgaga tcgcgacata caatgatcac
     9721 cggatggcga tgtgtttctc gctggtggcg ttgtcagata caccagtgac gattcttgat
     9781 cccaaatgca cggccaaaac atttccggat tatttcgagc agctggcgcg gattagccag
     9841 gcagcctgaa tgaacaacgg gcaataaata gccaaatctt tctttatcaa aacgtcggca
     9901 cattg
//
LOCUS       DAEBXN010000140         9774 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.140, whole genome shotgun sequence.
ACCESSION   DAEBXN010000140 DAEBXN010000000
VERSION     DAEBXN010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9774
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.140"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            392..2023
                     /gene="oppA"
                     /locus_tag="KJL99_003670"
     CDS             392..2023
                     /gene="oppA"
                     /locus_tag="KJL99_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HBD4438251.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLRYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            2109..3029
                     /gene="oppB"
                     /locus_tag="KJL99_003671"
     CDS             2109..3029
                     /gene="oppB"
                     /locus_tag="KJL99_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HBD4438252.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            3044..3952
                     /gene="oppC"
                     /locus_tag="KJL99_003672"
     CDS             3044..3952
                     /gene="oppC"
                     /locus_tag="KJL99_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HBD4438253.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            3964..4977
                     /gene="oppD"
                     /locus_tag="KJL99_003673"
     CDS             3964..4977
                     /gene="oppD"
                     /locus_tag="KJL99_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HBD4438254.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            4974..5978
                     /gene="oppF"
                     /locus_tag="KJL99_003674"
     CDS             4974..5978
                     /gene="oppF"
                     /locus_tag="KJL99_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HBD4438255.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(6031..6360)
                     /locus_tag="KJL99_003675"
     CDS             complement(6031..6360)
                     /locus_tag="KJL99_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HBD4438256.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEISDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6395..7855)
                     /gene="cls"
                     /locus_tag="KJL99_003676"
     CDS             complement(6395..7855)
                     /gene="cls"
                     /locus_tag="KJL99_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBD4438257.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAH
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            7998..8171
                     /locus_tag="KJL99_003677"
     CDS             7998..8171
                     /locus_tag="KJL99_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HBD4438258.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(8226..9479)
                     /gene="kch"
                     /locus_tag="KJL99_003678"
     CDS             complement(8226..9479)
                     /gene="kch"
                     /locus_tag="KJL99_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HBD4438259.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLLKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
BASE COUNT         2563 a         2285 c         2433 g         2493 t
ORIGIN      
        1 tgaaagatgt ttgttaaggc atggatgcaa gctatagatt ctgatacggt caataaaaga
       61 gaatcgctta acaattttgc aaaatgtatt ggcgagtaag aaccgcattt ggtactttcc
      121 gggcaaccgc cagacgattc tttattggta atgagaataa ttaacaatta aagagcgtcg
      181 cgaaagaata atgtgtctcg acaggggaga cacagtacga atcgacataa ggtgatcgtc
      241 tgaatcacca gaataaataa agtcggtgat agtaatacgt aacgataaag taacctgaca
      301 gcagaaagtc tccgagcttg tgcagggtcc caatccggga ttacacatgc tggttaatac
      361 cagtaattat aatgagggag tccaaaaaac aatgaccaac atcaccaaga gaagtttagt
      421 agcagctggc gttctggctg cgctaatggc agggaatgtc gcgctggcag ctgatgtacc
      481 cgcaggcgtc acactggcgg aaaaacaaac actggtacgt aacaatggtt cagaagttca
      541 gtcattagat ccgcacaaaa ttgaaggtgt tccggagtct aatatcagcc gagacctgtt
      601 tgaaggctta ctggtcagcg atcttgacgg tcatccagca cctggcgtcg ctgaatcctg
      661 ggataataaa gacgcgaaag tctggacctt ccatttgcgt aaagatgcga aatggtctga
      721 tggcacgcca gtcacagcac aagactttgt gtatagctgg caacgttctg ttgatccgaa
      781 cactgcttct ccgtatgcca gttatctgca atatgggcat atcgccggta ttgatgaaat
      841 tcttgaaggg aaaaaaccga ttaccgatct cggcgtgaaa gctattgatg atcacacatt
      901 agaagtcacc ttaagtgaac ccgttccgta cttctataaa ttacttgttc acccatcaac
      961 ttcaccggtg ccaaaagccg ctatcgagaa attcggcgaa aaatggaccc agcctggtaa
     1021 tatcgtcacc aacggtgcct ataccttaaa agattgggtc gtaaacgaac gaatcgttct
     1081 tgaacgcagc ccgacctact ggaacaacgc gaaaaccgtt attaaccagg taacctattt
     1141 gcctattgct tctgaagtta ccgatgtcaa ccgctaccgt agtggtgaaa tcgacatgac
     1201 gtataacaac atgccgatcg aattgttcca gaagctgaaa aaagagatcc cggacgaagt
     1261 tcacgttgat ccatacctgt gcacttacta ttacgaaatt aacaaccaga aaccgccatt
     1321 caacgatgtg cgtgtgcgta ccgcactgaa actaggtatg gaccgcgata tcattgttaa
     1381 taaagtgaaa gcgcagggcg acatgcccgc ctatggttat actccaccgt atactgatgg
     1441 cgcaaaattg actcagccag aatggtttgg ctggagccag gaaaaacgta acgaagaagc
     1501 gaaaaaactg ctggctgaag cgggttatac cgcagacaaa ccgttgacca tcaacctgcg
     1561 gtataacacc tccgatctgc ataaaaagct ggcgattgct gcctcttcat tgtggaagaa
     1621 aaacattggt gtaaacgtca aactggttaa ccaggagtgg aaaacgttcc tcgacacccg
     1681 tcaccagggc acttttgatg tggcccgtgc aggctggtgt gcagactaca acgaaccaac
     1741 ttccttcctg aacaccatgc tgtcgaacag ctcgatgaat accgcgcatt ataagagccc
     1801 ggcctttgac agcattatgg cggaaacgct gaaagtgact gacgaggcgc agcgcacagc
     1861 tctgtacact aaagcagaac aacagctgga taaggattcg gccattgttc ctgtttatta
     1921 ctacgtgaat gcgcgtctgg tgaaaccgtg ggttggtggc tataccggca aagatccgct
     1981 ggataatacc tatacccgga atatgtacat tgtgaagcac taatggcaat acgtggggca
     2041 ggagtgtcct gctccacggt gtctgatttt tatcgcatta cagaaggcac aggccagaag
     2101 gtagggcaat gttaaaattt attctacgtc gttgtctgga agcgattccg acgctattta
     2161 ttcttattac tatttcgttc tttatgatgc gcctcgcacc gggaagccct tttaccggcg
     2221 aacgtacttt accgccagaa gtgatggcca atatcgaagc gaaatatcat cttaatgatc
     2281 cgatcatgac acagtatttc agctacctga aacaactggc gcatggcgat ttcggtccat
     2341 cgtttaaata taaagattat tcggtcaatg acctggtggc atccagtttt cccgtttctg
     2401 ccaaactggg agccgccgca tttttccttg cggtaatact gggtgttagt gctggcgtta
     2461 ttgccgcatt aaaacaaaac accaaatggg actataccgt gatggggctg gcaatgaccg
     2521 gggttgttat ccccagtttt gtggttgcgc cattattagt catgatattt gcgatcattt
     2581 tgcattggct gccgggcggt ggctggaatg gtggggcgct taaattcatg atattgccga
     2641 tggtggcgtt gtcactcgcc tatatcgcca gtattgcgcg tattacccgt ggctctatga
     2701 ttgaagtatt acactccaac tttattcgta ctgcccgggc gaaagggtta cctatgcggc
     2761 ggatcatttt acgccatgca ttaaaacctg ctctgttacc cgtgctctcc tatatgggcc
     2821 ccgcatttgt cggcattatt accggttcta tggtcatcga aaccatttat ggtttgccgg
     2881 ggattgggca actgttcgtt aatggcgcat tgaaccgtga ctattcctta gtgttaagcc
     2941 tgaccatcct ggttggtgcg ttaaccattt tgtttaatgc cattgtcgat gtgctatatg
     3001 cggttatcga cccgaaaatc cgttactgat actggagctc gcgatgatgt taagtaagaa
     3061 aaacagcgag acgctggaaa atttcagtga aaagctggag gtcgaagggc gcagcttgtg
     3121 gcaggacgca cgtcgacgtt ttatgcataa ccgtgcggcg gttgccagtc tgatagtgct
     3181 ggtgctgatc gcgttatttg taatcctggc accgatgctt tcccagtttg cctatgacga
     3241 tactgactgg gcgatgatgt ccagcgcccc ggatatggag tccggtcact actttggtac
     3301 tgactcatcc ggtcgcgacc tgcttgtgcg cgttgcgatt ggcgggcgta tctcactcat
     3361 ggtcggtgtt gctgcggcac tggtggcagt ggtcgtgggg acactttatg gttcgctttc
     3421 cggttatctg ggcggtaaag tggattcggt aatgatgcgt ctgctggaaa tcctcaactc
     3481 cttcccattc atgttcttcg tcattttgct ggtgaccttt ttcggccaaa acatcctgct
     3541 gattttcgtg gcgattggca tggtttcctg gctggatatg gctcgtattg tgcgtgggca
     3601 aaccctgagt ctgaagcgca aagagtttat tgaggcggca caagttggcg gtgtatcgac
     3661 gccgggcatt gttattcgcc acattgtgcc gaacgtactc ggtgtggtgg tggtctacgc
     3721 atcgctactg gtgcccagca tgatcctctt tgaatctttc cttagcttcc tggggttggg
     3781 tacgcaagag ccgttaagca gctggggggc attgctgagt gatggcgcga actcgatgga
     3841 agtctctcca tggttactgt tgttcccagc gggattcctt gtggtgacac tgttttgttt
     3901 caactttatc ggcgatggct tgcgtgatgc cctcgacccg aaagatcgtt aaggagtgca
     3961 gccatgagcg taattgaaac tgcaactgtg ccgctcgcac aacaacaggc tgacgcactg
     4021 ctgaacgtga aagatttgcg tgtcaccttt agtaccccgg acggcgacgt cacggcggtc
     4081 aatgatttaa atttttccct acgtgccgga gaaacgctgg gcattgtagg tgagtctggt
     4141 tcgggtaaat cgcaaactgc atttgcgttg atgggcctgt tggctgccaa cggacgcatt
     4201 ggcggatcgg caaccttcaa tgggcgtgaa atcctcaatt tgccagagcg tgaactcaat
     4261 aaactgcgcg ctgaacaaat ctcaatgatt tttcaggacc caatgacttc gttgaatccc
     4321 tatatgcgcg tcggtgagca gttgatggaa gtgctgatgc tgcataagaa catgagcaaa
     4381 gctgaggcgt ttgaagagtc ggtgcggatg ctcgatgcgg taaaaatgcc ggaagcacgt
     4441 aaacgcatga aaatgtaccc gcacgaattt tctggcggca tgcgtcagcg agtcatgatt
     4501 gcgatggcat tgctatgtcg acctaagttg ctgattgcgg atgaacccac tacagcgctg
     4561 gacgtcaccg tacaggcgca gatcatgacg ctattgaatg aactgaagcg ggaatttaat
     4621 actgccatca ttatgatcac ccacgatctt ggcgtggtgg cggggatctg tgacaaagtg
     4681 ctggtaatgt acgccgggcg cacgatggaa tatggcaatg cgcgcgatgt cttttatcaa
     4741 cccgttcatc cttattctat cggtttgctc aacgcggtgc cgcgtcttga tgcggaaggt
     4801 gaaacaatgt tgaccatccc gggtaatccg ccaaacttgc tgcgattacc gaaaggttgc
     4861 ccgttccagc cacgttgtcc acatgcgatg gaaatttgta gtagcgcacc gccgctggaa
     4921 gagtttacgc ctggccgtct gcgtgcttgc tttaaaccgg tggaggaact gttatgaatg
     4981 ctgtaactga aggaagaaaa gtcctccttg aaatcgccga tcttaaagtg cactttgaaa
     5041 tcaaagatgg caaacagtgg ttctggcaac cgccgaaaac gctcaaagcc gtcgatggtg
     5101 tcactcttcg cctgtatgaa ggggaaacat taggtgtggt aggggaatcg ggatgcggta
     5161 agtccacctt tgctcgcgcc atcatcggtt tggtcaaggc gaccgacggt catgttgcct
     5221 ggttaggtaa agagttgctg ggcatgaagc ccgatgaatg gcgtgccgtt cgcagtgata
     5281 ttcagatgat tttccaggat ccgttggcat cgctaaaccc gcgtatgacc atcggcgaga
     5341 tcatcgctga accactgcgt acttatcatc cgaaaatgtc acgccaggaa gttcgcgagc
     5401 gcgtgaaggc gatgatgctg aaagtcgggt tattgcctaa cctgattaac cgctatccgc
     5461 atgagttctc cggtgggcag tgccagcgta tcgggattgc tcgtgctctt attcttgaac
     5521 cgaagctgat tatctgcgat gagccggttt cggcgctgga cgtgtcaatt caggcgcagg
     5581 tggttaacct gctccagcag ctgcaacgtg agatgggatt gtcattaatt tttattgctc
     5641 atgacctggc cgtggtaaaa cacatttccg atcgtgtgtt ggtgatgtat ctcggccatg
     5701 cagtagaact ggggacctat gatgaggtct accacaatcc actacatcct tacaccaggg
     5761 cattgatgtc ggcagtcccc atacctgatc cggatctgga gaagaacaaa accatccagt
     5821 tactggaagg ggaattaccg tcgccgatca acccgccttc cggttgtgtt ttccgtaccc
     5881 gttgcccgat tgccggtccg gagtgcgcca aaacacgtcc tgttctggag gggagtttca
     5941 gacacgccgt ttcttgcctg aaagtcgatc cgctttaaaa acaataaggg ctgacagttg
     6001 tcagcccttt ttcacgctaa aagcgattat ttattcccgc cagatgatat ggcaaagttt
     6061 gtgatctttt tcgcggcata acagaatgcg ggcgaaaaca tcgctgattt caccatcttc
     6121 actgtccgcc agaccaatca ccacttcggc aaaaaagtcc gggttcaaat cgaaatcaac
     6181 atgttcctgc cagtcttccg ccggatcgaa taactccgcg ccgccgcgct cttcaaactg
     6241 aagattgaac agcagaacat cagctggatc gagattgtcg gcagccagtt cgagaaaaat
     6301 atcgtaggcc tgctcaagcg tttcatcttc agtcaggcga ttgtttagat ccatatccat
     6361 agtcactacc tgtttaacct ctgttggcga cgttttacag caacgggctg aagaagtaaa
     6421 acagtcgctc ggcgacacgt tgccataatg gacgttttag ccataaacgg gcatcgagca
     6481 gacgtgaacg cgaaatataa tcgtcctgaa cggcggcaag gtctgcacca aaacctttat
     6541 cgtcgattgc caaggtaatc tcgaaattca gccacagact acgcatatca aggttaacgg
     6601 tgccaaccag acttagttcg ccatcgacca gcacgctctt ggtatgcagt aacccgcctt
     6661 caaactgata aattttaacc ccagcagcaa gcagttccgt aaagaatgcg cgactggccc
     6721 agccgaccag catcgagtca ttttttcgcg gaaggataat actgacatcc accccgcgct
     6781 gcgccgccgt gcaaatcgca tgaagtaaat catcgcttgg cacaaagtag ggcgtggtca
     6841 tgatcaaata ttcatgcgcc gaataagccg cagtcaataa tgcctggtga atgagatctt
     6901 ccggaaagcc ggggccagaa gcaattgtgt gaatggtgtg accgctggcc tgttcaaacg
     6961 gcataatatt gacatctggt ggtggcggca gaatacgttt tccggtttca atctcccagt
     7021 cgcaggaata aataatcccc atcgcggtgg cgatggggcc ttccatacgc gccatcagat
     7081 caatccattg ccctacgccc gcatcttgtt tgaagtagcg aggatcgacc atattcatgc
     7141 tgccggtgta cgcgatgtaa ttatcgatca tgatcatctt gcgatgttgg cgcaggtcca
     7201 tacggcgtaa aaacacacgc atcagattga cctttaaggc ttcgaccact tcaataccgg
     7261 cattacgcat tagctcgggc cacgggctgc ggaaaaaagc cacactcccg gcggagtcga
     7321 gcatcaatcg gcaatgaata ccacgccgtg cagccgccat taatgattca gccacctggt
     7381 ccgccatgcc gccgggctgc cagatataaa acaccatctc aatattatgg cgcgcgagct
     7441 ggatgtcgcg gattaacgcc tgcatcacat catctgactc ggtcatcagc tgtagctgat
     7501 tccctttgac cccagcgatc ccctgacgac gctcgcaaag cttgaataat ggcgcagcga
     7561 cactgctatt ttcttcggcg aagatatgct tacaggcttt aaggtcgtta agccattttg
     7621 cggtggaagg ccacatcgct ctggcgcgct cagcgcggcg tttgcctaaa tggagctcgc
     7681 caacggcaag ataggcaata attccgacta acggcagaat gtaaataatc aacagccagg
     7741 ccatcgcgga gggaactgcg cgtcgtttca ttagaatgcg taaagttacg cctgcaatga
     7801 gcaaccagta tcccagaatg gccaaccaac tcaccaacgt ataaacggtt gtcatagatt
     7861 agaaatcctt ttgaaagcgc attgttatga gtttacgcat atggattcat ctggcaaata
     7921 aaaacgcggg aaaagcactg gtcagcgtcg ggggcggggg tataatgacc attctgttat
     7981 tgcatagagt agttaacatg aagcggagta gaacggaagt ggggcgctgg cgcatgcagc
     8041 gtcaggctag ccgacgtaaa tcgcgttggc ttgaggggca atcgcgccga aatatgcgta
     8101 tccacagcat caggaagtgc attctaaaca aacagcgtaa ctcgttattg tttgcgatct
     8161 acaatatcta aatgtactgg gcaccattgc ggtgcccaat aagtttactc ttccgtcttt
     8221 aaactctatt tttgcgccga ttctttactg tcagctttcg tttcttgttc atcgttatcg
     8281 ctgaaaatac catgacctga gttcaacaac attgaaacga gcatatcatt attaatctct
     8341 tcaccattta aaactcgcgc cagaatttcg ctgccaaaca gttgcggtga gagaatgata
     8401 tccggatgta ccatcttaat cttattcagg tttttgctat cactgacggc gagaactgtt
     8461 ttgacatcac tgctcatatc ttttgccgag agtacaacaa acgcgttatc tgcatcgtta
     8521 tcactcagcg ccagaatggc ccggcaacga tcgattcccg ctttctttaa tactgaactg
     8581 tcattactgt caccggggat aacatcagcg ttatcgccta agcgttgctc aagttgcttg
     8641 atatcatctt caggcaagtt gctgataacc gttacgtttt gtccgcgttg attcagttgc
     8701 aggatcgtat tgatggcgag aatcgaatgt ccgcaaacaa taaaatgatc tttacgatgc
     8761 attgtatgat tgtttccttt taaaagtttg ttgaatcccc cgcggataag cggaccaaaa
     8821 attgaggtca tagatgtggc aaaaacggta atgccggaaa taatgaccga aatagtgaac
     8881 aatcgtgctg attcagaaac agggacaata tctccatagc cgacggttga catggtttct
     8941 atcgaaaaat agaacgcggt catcaaactt tctattcgcg gattaaaacc ttcgcttaaa
     9001 taaagcgcac cgtaggttga gtaaaacagt aatgtcgtga aactaataaa tgcaaaaatt
     9061 gtcccggctg cggcgctact gtgggagaag tctttgcgca gtatcagcaa aaacaccagc
     9121 gtaaaaatgc aaaatccaat actaaatttc agccagggat aaaaatgcag ggtgtaaatt
     9181 agcgctatca acaacaaaat gatactgatt gcccatgcga gctttgcccg gaacagtaag
     9241 ccaatggagt tcaggacaag aaagacgccg agcataaata gtggggcgtt tgccagcaag
     9301 gagagatcca actctgaaaa tgctttgata tggaaaatat caagcaaatt gaccgacata
     9361 ccatagattg ttttaaaaat aagcaatcca tttaaaaaga cggccagcgc gagaatgtcg
     9421 tgccgtaggg tcacccataa atttgttgct gtttgtttga atgtagccca gtgactcact
     9481 gatacctctg taaccacaat tttgtttaat tatcttagcg tgcacatttt ctgaacatac
     9541 atgcagcgtg tgaatgtgtt aaacaatagt agatgacttt atccgcagta atcaaaggtt
     9601 ttgagtgagc aactctgcgt caggaaaggg cttttttcct gaacatgctc acatcatata
     9661 aagaaataca gataaagtta ttatctgctt gtggtagtga atgcactgac cggctataag
     9721 gaaaggccaa acaagaacac ggttgcaaaa accgtgccct taaatattgg atct
//
LOCUS       DAEBXN010000141         9681 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.141, whole genome shotgun sequence.
ACCESSION   DAEBXN010000141 DAEBXN010000000
VERSION     DAEBXN010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9681
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.141"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(26..214)
                     /locus_tag="KJL99_003679"
     CDS             complement(26..214)
                     /locus_tag="KJL99_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HBD4438260.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMVMTPTY"
     gene            complement(433..1410)
                     /gene="epmA"
                     /locus_tag="KJL99_003680"
     CDS             complement(433..1410)
                     /gene="epmA"
                     /locus_tag="KJL99_003680"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HBD4438261.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1735..3543
                     /gene="frdA"
                     /locus_tag="KJL99_003681"
     CDS             1735..3543
                     /gene="frdA"
                     /locus_tag="KJL99_003681"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HBD4438262.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAINMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            3536..4270
                     /gene="frdB"
                     /locus_tag="KJL99_003682"
     CDS             3536..4270
                     /gene="frdB"
                     /locus_tag="KJL99_003682"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HBD4438263.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4281..4676
                     /gene="frdC"
                     /locus_tag="KJL99_003683"
     CDS             4281..4676
                     /gene="frdC"
                     /locus_tag="KJL99_003683"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HBD4438264.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FALFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4687..5046
                     /gene="frdD"
                     /locus_tag="KJL99_003684"
     CDS             4687..5046
                     /gene="frdD"
                     /locus_tag="KJL99_003684"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HBD4438265.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            5109..6242
                     /locus_tag="KJL99_003685"
     CDS             5109..6242
                     /locus_tag="KJL99_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HBD4438266.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6331..6864
                     /gene="blc"
                     /locus_tag="KJL99_003686"
     CDS             6331..6864
                     /gene="blc"
                     /locus_tag="KJL99_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HBD4438267.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6861..7178)
                     /gene="sugE"
                     /locus_tag="KJL99_003687"
     CDS             complement(6861..7178)
                     /gene="sugE"
                     /locus_tag="KJL99_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HBD4438268.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7354..7500)
                     /gene="ecnB"
                     /locus_tag="KJL99_003688"
     CDS             complement(7354..7500)
                     /gene="ecnB"
                     /locus_tag="KJL99_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HBD4438269.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7611..7736)
                     /gene="ecnA"
                     /locus_tag="KJL99_003689"
     CDS             complement(7611..7736)
                     /gene="ecnA"
                     /locus_tag="KJL99_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HBD4438270.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(7788..8354)
                     /gene="efp"
                     /locus_tag="KJL99_003690"
     CDS             complement(7788..8354)
                     /gene="efp"
                     /locus_tag="KJL99_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBD4438271.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8396..9424
                     /gene="epmB"
                     /locus_tag="KJL99_003691"
     CDS             8396..9424
                     /gene="epmB"
                     /locus_tag="KJL99_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HBD4438272.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKEHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2465 a         2480 c         2466 g         2270 t
ORIGIN      
        1 ggcattatct gaacataaaa cactatcagt aagttggagt cattaccatt aaggcgaatg
       61 gaataaagaa taacaatgca gaaaatatat aaaagggaat cgcactataa cccattaagt
      121 aataagccga tgggctattg gcaaatccaa atacggaagt aaagatcatc aatatgagtc
      181 ctatcagact catctttttt atcgtgtgag gcatgaaacc atccttcatt tcagcgcgtg
      241 tataacacgt gcccactgaa ctgcactgcg caacattgag caggcagcgt aaaaaccaat
      301 acagaattga tacttggatg atatcaaata tccagtctta aattgtggcc tttaacggaa
      361 aaatgaaaac atatgctgca gaatggcgct tatcacgcca ttcttcgctg ttaattcagt
      421 aatttttcag aattatgccc ggtcaacgct aaaggcgatg acttcagcca gtgtctccgc
      481 gcccagcgcc aacatcacca gacgatcaac acctaatgcc acgccggaac agtcaggcat
      541 accgactttc aaggcttcaa tcagattctg gtcaatgggg tgctgcggca aaccgcgcgc
      601 cgcgcgctta cggttatctt gttcaaagcg ttgttgctgc tcacgggcat ccgttaattc
      661 atggaaacca ttcgccagct caataccttt ataataaacc tcaaagcgtt cagcgacccg
      721 atgatcttcg gtactgattt gcgccagtga tgcctggctg gctggaaagt ggtacacaaa
      781 ggtcggtttt tctttaccaa tatttggctc tacgccaaag gtaaacagca attgtagcaa
      841 cgtgtcgcgg tcttcttcgg tatcagcaac attgctcaaa tccagtttcg ctgcgacttc
      901 ccgcagttgc gttttgtcgg cagagagcgg gtcaatttcc agataacgca agaaagcttg
      961 ttgataagaa aggctttctg ctgccgggca gtccagcacc tgttgtaaga gatcgtccac
     1021 ctcgttcatc aaccggtaca tatcatagtg cggtcgatac cactccagca tagtgaactc
     1081 agggttgtga taacgcccca tctcttcatt acggaagctg cggcacagct ggaataccgg
     1141 cccacaacca gcaaccagca ggcgtttcat atggtattcc gggctggtca ttaaccagag
     1201 attcatcccc tgcgaatgcc cggggccaac gaaacgtgtc tcaaacggga ccaaatgaat
     1261 atcggttacc gtcgcctggc tcatacaagg cgtctccacc tccagcactc cacgatcggc
     1321 aaagaaacga cggatctccg ccataatcgc cgcgcgtttt aataagttag gaatggatgc
     1381 gctcggctgc caggatgccg tttcgctcat agttaaatct ccagtttttg acaagggcac
     1441 gaagtctact cgcaacgcga cggcgagaca aattttacgc aggaatcaaa cagcggtggg
     1501 cagtgactaa aaaaagcacg atctgatggt ttagtaatta aattaatcat cttcagtgat
     1561 aatttagccc tcttgcgcac taaaaaaatc gatctcgtca aatttcagac ttatccatca
     1621 gactatactg ttgtacctat aaaggagcag tggaatagcg ttcgcagacc gtaactttca
     1681 ggcacttacc ctgaagtacg gggctgtggg ataaaaacaa tctggaggaa tgtcgtgcaa
     1741 acctttcaag ccgatcttgc cattgtaggc gccggtggcg cgggattacg tgctgcaatt
     1801 gctgccgcgc aggcaaatcc aaatgcaaaa atcgcactaa tctcaaaagt atacccgatg
     1861 cgtagccata ccgttgctgc agaagggggc tccgccgctg tcgcgcagga tcatgacagc
     1921 ttcgaatatc actttcacga tacagtagcg ggtggcgact ggttgtgtga gcaggatgtc
     1981 gtggattatt tcgtccacca ctgcccaacc gaaatgaccc aactggaact gtggggatgc
     2041 ccatggagcc gtcgcccgga tggtagcgtc aacgtacgtc gcttcggcgg catgaaaatc
     2101 gagcgcacct ggttcgccgc cgataagacc ggcttccata tgctgcacac gctgttccag
     2161 acctctctgc aattcccgca gatccagcgt tttgacgaac atttcgtgct ggatattctg
     2221 gttgatgatg gtcatgttcg cggcctggta gcaataaaca tgatggaagg cacgttggtg
     2281 cagatccgtg ctaacgcggt cgttatggct accggcggtg cgggtcgcgt ttatcgttac
     2341 aacaccaacg gcggcatcgt taccggtgac ggtatgggta tggcgctaag ccacggcgtt
     2401 ccgctgcgtg acatggaatt cgttcagtat cacccaaccg gtctgccagg ttccggtatc
     2461 ctgatgaccg aaggctgccg tggtgaaggc ggtattctgg tcaacaaaaa tggctaccgt
     2521 tatctgcaag attacggcat gggcccggaa actccgctgg gcgagccgaa aaacaaatat
     2581 atggaactgg gtccacgcga caaagtttct caggccttct ggcacgaatg gcgtaaaggc
     2641 aacaccatct ccacgccgcg tggtgatgtg gtttatctcg acctgcgtca cctcggcgag
     2701 aaaaaactgc atgaacgtct gccgttcatc tgcgaactgg cgaaagcgta cgttggcgtc
     2761 gatccggtta aagaaccgat tccggtacgt ccgactgcac actacaccat gggcggtatc
     2821 gaaaccgatc agaactgtga aacccgcatt aaaggtctgt tcgccgtggg tgaatgttcc
     2881 tctgttggtc tgcacggtgc aaaccgtctg ggctccaact ccctggcgga actggtggtc
     2941 ttcggccgtc tggccggtga acaagcgaca gagcgtgcag caactgccgg taatggcaac
     3001 gaagcggcaa ttgaagcgca ggcagctggc gttgaacaac gtctgaaaga tctagttaac
     3061 caggatggcg gcgaaaactg ggcgaagatc cgcgacgaaa tgggcctggc aatggaagaa
     3121 ggttgcggta tctaccgtac gccggaactg atgcagaaaa ccatcgacaa gctggcagag
     3181 ctgcaggaac gcttcaagcg cgtgcgcatc accgacacct ccagcgtgtt caacaccgac
     3241 ctgctctaca ccattgaact gagccacggt ctgaacgttg ctgaatgtat ggcgcactcc
     3301 gcaatggcac gtaaagagtc ccgcggcgca caccagcgtc tggacgaagg ttgcaccgag
     3361 cgtgacgacg tcaactttct caaacacacc ctcgccttcc gcgatgctga tggcacgact
     3421 cgcctggagt acagcgacgt gaagattact acgctgccgc cagctaaacg cgtttacggt
     3481 ggcgaagcgg atgcagccga taaggcggaa gcagccaata ataaggagaa ggcgaatggc
     3541 tgagatgaaa aacctgaaaa ttgaggtggt gcgctataac ccggaagtcg ataccgcacc
     3601 gcatagcgca ttctatgaag tgccttatga cgcaactacc tcattactgg atgcgctggg
     3661 ctacatcaaa gacaacctgg caccggacct gagttaccgc tggtcctgcc gtatggcgat
     3721 ttgtggttcc tgcggcatga tggttaacaa cgtgccaaaa ctggcatgta aaaccttcct
     3781 gcgtgattac accgacggta tgaaggttga agcgttagct aacttcccga ttgaacgcga
     3841 tctggtggtc gatatgaccc acttcatcga aagtctggaa gcgatcaaac cgtacatcat
     3901 cggcaactcc cgcaccgcgg atcagggtac taacatccag accccggcgc agatggcaaa
     3961 gtatcaccag ttctccggtt gcatcaactg tggtctgtgc tacgccgcgt gcccgcagtt
     4021 tggcctgaac ccagagttca tcggtccggc tgccattacg ctggcgcatc gttataacga
     4081 agatagccgc gaccacggta agaaggagcg tatggcgcag ttgaacagcc agaacggtgt
     4141 atggagctgt actttcgtgg gctattgctc cgaagtctgc ccgaaacacg tcgatccggc
     4201 tgcggccatt cagcagggca aagtagaaag ttcgaaagac tttcttatcg cgaccctgaa
     4261 accacgctaa ggagtgcaac atgacgacta aacgtaaacc gtatgtacgg ccaatgacgt
     4321 ccacctggtg gaaaaaattg ccgttttatc gcttttacat gctgcgcgaa ggcacggcgg
     4381 ttccggctgt gtggttcagc attgaactga ttttcgcgct gtttgccctg aaaaatggcc
     4441 cggaagcctg ggcgggattc gtcgactttt tacaaaaccc ggttatcgtg atcattaacc
     4501 tgatcactct gacggcagct ctgctgcaca ccaaaacctg gtttgaactg gcaccgaaag
     4561 cggccaatat cattgtaaaa gacgaaaaaa tgggaccaga gccaattatc aaaagtctct
     4621 gggcggtaac tgtggttgcc accatcgtaa tcctgtttgt tgccctgtac tggtaaggag
     4681 cctgagatga ttaatccaaa tccaaagcgt tctgacgaac cggtattctg gggcctcttc
     4741 ggggccggtg gtatgtggag cgcaattatt gcgccggtga tgatcctgct ggtgggtatt
     4801 ctgctgccac tggggctgtt tccgggtgat gcgctgagct acgagcgcgt tctggcgttc
     4861 gcgcagagct tcattggtcg cgtattcctg ttcctgatga tcgttctgcc gctgtggtgt
     4921 ggtttacacc gtatgcacca cgcgatgcac gatctgaaaa tccacgtgcc tgcgggcaaa
     4981 tgggttttct acggtctggc tgctatcctg acagttgtca cgctgattgg tgtcgttaca
     5041 atctaacgca tcgccaatgt aaatccggcc cgcctatggc gggccgtttt gtatggaaac
     5101 cagaccttat gttcaaaacg acgctctgcg ccttattaat taccgcctct tactccacat
     5161 ttgctgcccc tcaacaaatc aacgatattg tgcatcgcac aattaccccg cttatagagc
     5221 aacaaaagat ccccggtatg gcggtggcgg taatttatca gggtaaacct tattacttta
     5281 cctggggcta tgcggacatc gccaaaaagc agcccgtcac acagcaaacg ttgtttgagt
     5341 taggttcggt cagcaaaaca tttacgggcg tgcttggtgg cgacgctatt gctcgagggg
     5401 aaatcaagtt aagcgatccc gcaacaaaat actggcctga acttaccgct aaacagtgga
     5461 atgggatcac actattacat cttgcgacct acaccgctgg cggcctgcca ttgcaggtgc
     5521 cggatgaagt gaaatcctca agcgacttgc tgcgcttcta tcaaaactgg cagcctgcat
     5581 gggctccagg aacacaacgt ctgtatgcca actccagtat cggtttgttc ggcgcactgg
     5641 ctgtgaagcc gtctggtttg agttttgagc aggcgatgca aactcgtgtc ttccagccac
     5701 tcaaactcaa ccatacgtgg attaatgtac ctcccccaga agaaaagaat tacgcctggg
     5761 gatatcgcga aggtaaggca gtgcatgttt cgccaggggc gttagatgct gaagcttatg
     5821 gtgtgaagtc gaccattgaa gatatggccc gctgggtgca aagcaattta cccccccttg
     5881 atatcaatga gaaaacgctt caacaaggga tacaactggc acaatctcgc tactggcaaa
     5941 ccggcgatat gtatcagggc ctgggctggg aaatgctgga ctggccggtc aatcctgaca
     6001 gcatcattaa cggcagtgac aataaaattg cactggcagc acgcccggta aaagcgatta
     6061 agcccccaac tcctgcagta cgcgcatcat gggtacataa aacgggggcg accggcggat
     6121 ttggtagcta tgtcgcgttt attccagaaa aagagctggg tatcgtgatg ctggctaaca
     6181 aaaactatcc caatccagcg agagtcgccg ccgcctgtca gattctcaac gctctacagt
     6241 aaaatttcat cgggtccgaa ttttcggacc ttttctgcgc ttttccttgc tgtcatctac
     6301 acttagaaaa aaaccagtaa ggaaacattt atgcgcctgc tccctctcgt tgccgcagcg
     6361 acagctgcat ttctggtcgt tgcctgtagt tttcctacgc cgccgcgtgg cgtgaccgta
     6421 gtgaataatt tcgacgccaa acgttatctc ggtacctggt atgagattgc ccgttttgat
     6481 caccgttttg aacgtggact ggaaaaagtc accgcaacat acagcctgcg tgatgacggc
     6541 ggcctgaatg tcattaataa aggctataac cctgacagag gaatgtggca gcagagtgaa
     6601 gggaaagcgt actttaccgg cgcaccaact cgcgctgcgc tgaaagtgtc attctttggt
     6661 cctttctatg gcggttataa cgttattgca ctcgatcggg aataccgcca tgcgctggtt
     6721 tgcggcccgg accgcgacta cctgtggata ctctcccgca cgccaaccat ttctgacgaa
     6781 gtgaaacagg agatgctggc agtcgcgacc cgggaagggt ttgatgtcag taagtttatt
     6841 tgggtacagc agcccggtag ttagtgagtg ctgagtttca gaccaataat ccccaatacg
     6901 attaacgcca gactcgccag acgcatcggg ttagcggact caccgagcag cacaatgccg
     6961 gtgatggccg cgccgactgc gccaataccc gtccacacgg cataagccgt ccctaccggt
     7021 aacgatttca tcgcccaggc aagtagcgcc atactgacta tcatcgccgt cacggtaata
     7081 acactcggcg tcaaacggct aaagccgtgg gtatatttca ggccaacggc ccataccact
     7141 tccagcagac cagcaataac taagataatc caggacatat caggctccgg aaatttgggg
     7201 ccgtccccgg tgaaaagagg cgtttgcagg tcgtcctgca aagctgatgg ttgaacggat
     7261 attttgccag gtggcagggg aatttcaact attaccttta tgacgatcgc agaaaaaaca
     7321 aacggcacga cactgctgtc gtaccgttat tgcttattgc tgcgctttcg ttgctgcgcc
     7381 agaaatcgcg ttaccgccat cagaaatgtc ttcaccaacg ccacgcgtgg tgttgcaggc
     7441 agttaatact gttgaaagca ccagaacaga aaagatcgct gcaattgtct tcttcaccat
     7501 aacgtcttcc ttttaggtaa tgttatttat gtttgcctat agcaaattaa taatagacaa
     7561 catcgccaaa aatgatggaa atctgatgat ttttcggaag agaagaaaaa ttagctggca
     7621 gcgcgagaga tggagttacc gagatgtttg atgtcttcgc cgaaaccgca agcggtgtta
     7681 cagcccgtga gcagcgtgct ggcaagcaaa acaagaacga taaggcgttt catcatctct
     7741 gacctgaata agtgatggtg cagcctgcag gccgcaccac aaccgcatta cttcacgcga
     7801 gagacgtatt caccagagcg ggtatccact ttgatgactt cgccgatttg tacgaacagc
     7861 ggaactttaa ccacagcgcc agtagacagg gtagccggtt tgccgccagt acctgcggta
     7921 tcacctttca ggcccggatc ggtatcaacg atttccagtt caacgaagtt cggcggagta
     7981 acggagatcg gctgaccatt ccacagagtt acgatacact ctgcctgatc cagcagccat
     8041 ttagcattgt cgccaattgc ttttgcatca gcagacagct gctcgaaagt ttcgttgttc
     8101 atgaagtgcc agaactcacc gtcgttgtac aggtaagtca ggttcatatc gacaacatca
     8161 gcgccttcag cggaatcagt agatttgaag gttttttcta cgcgagtacc ggtcagcaga
     8221 cgacgcagtt taacgcgagc aaatgcctgg cctttacccg gttttacgaa ttcactcgct
     8281 tcaaccgcgt aaggttcgcc gtctaacatg attttaagac cagcacgaaa atcgttgcta
     8341 tagtacgttg ccataaggcc ctctgaaatt tgttaattgg tagctaagcc acaaaatggc
     8401 gcatattgta accctaaata ccccatccag agaagattgg ttaacgcaac ttgccgatgt
     8461 tgtgaccgat cctgatgaac ttctgcgtct tttgaatata gacgcggacg aaaaactgtt
     8521 ggccggacgc agcgccaaaa agctgtttgc cctgcgtgtg ccccgctcat ttatcgatcg
     8581 catggagaaa ggcaatccgg acgatcctct tttgcgtcag gtacttacct cgcaagatga
     8641 gtttgtcgtc gcgtccggat tctccaccga cccactggaa gaacagcaca gcgtagtgcc
     8701 tggtttgttg cataaatacc acaaccgggc gcttttgctg gtcaaaggcg gctgcgcggt
     8761 aaattgccgc tattgcttcc gtcgccactt cccctatgcc gaaaatcagg gcaacaagcg
     8821 taactggcag actgcacttg agtatgttgc tgcgcatccg gaactggacg agatgatttt
     8881 ctccggcggc gatccgctga tggcgaaaga gcacgagctg gactggttgc tcacacaact
     8941 ggaagccatc ccgcatatca aacgtctgcg gattcacagc cgtctgccga ttgtgatccc
     9001 ggcacgtatc accgaggcgc tggttgaacg ctttgcccgt tctacgctgc aaatcttgct
     9061 ggtgaatcac atcaatcatg ccaatgaggt agatgaaacg ttccgtcagg cgatggctaa
     9121 attgcgccgt gtcggtgtca ccctgcttaa ccagagcgtt ctgttacgtg gtgtgaacga
     9181 taacgcacaa acgttggcaa acctgagtaa tgcgttgttc gatgccggcg tgatgccgta
     9241 ttacctgcat gtgctagata aagtacaggg cgcggcgcat tttatggtga gtgatgacga
     9301 agcacggcag attatgcgtg agttgctgac attggtgtcg ggatatctgg tgccgaaact
     9361 ggcgcgagaa attggcggcg aacccagcaa aacgccgctg gatctccagc tacgccagca
     9421 gtaactgatt aaacagggaa gttaaacatt aaaattgcct gatgcgctac gcttatcagg
     9481 cctacatcgt gcattgaaat tgaatggatt tgcaatgaac tgtaggccgg ataaggcgtt
     9541 tacgccgcat ccggcaagag caacaaacgc aagtacagca accactgaga aatttaatct
     9601 ttgcacgttg cataaccttt aattattcat atgcattgct gttgttactt tttatatagc
     9661 taacaccaat aattaacctt t
//
LOCUS       DAEBXN010000142         9572 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.142, whole genome shotgun sequence.
ACCESSION   DAEBXN010000142 DAEBXN010000000
VERSION     DAEBXN010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9572
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.142"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..133
                     /locus_tag="KJL99_003692"
     CDS             <1..133
                     /locus_tag="KJL99_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBD4438273.1"
                     /translation="SNISELKDAVTEYIEYYNSRRISLKLKGLTPIEYRNQTYMPRV"
     gene            230..382
                     /gene="hokE"
                     /locus_tag="KJL99_003693"
     CDS             230..382
                     /gene="hokE"
                     /locus_tag="KJL99_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HBD4438274.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLAGDSLCEFTVKERNIEFRAVL
                     AYEPKK"
     gene            733..885
                     /gene="hokE"
                     /locus_tag="KJL99_003694"
     CDS             733..885
                     /gene="hokE"
                     /locus_tag="KJL99_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HBD4438275.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(1007..1627)
                     /gene="entD"
                     /locus_tag="KJL99_003695"
     CDS             complement(1007..1627)
                     /gene="entD"
                     /locus_tag="KJL99_003695"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HBD4438276.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(1802..4042)
                     /gene="fepA"
                     /locus_tag="KJL99_003696"
     CDS             complement(1802..4042)
                     /gene="fepA"
                     /locus_tag="KJL99_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HBD4438277.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKIGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKVETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SINTHF"
     gene            4285..5487
                     /gene="fes"
                     /locus_tag="KJL99_003697"
     CDS             4285..5487
                     /gene="fes"
                     /locus_tag="KJL99_003697"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HBD4438278.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGLGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKVIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            5490..5708
                     /gene="ybdZ"
                     /locus_tag="KJL99_003698"
     CDS             5490..5708
                     /gene="ybdZ"
                     /locus_tag="KJL99_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HBD4438279.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            5705..9550
                     /gene="entF"
                     /locus_tag="KJL99_003699"
     CDS             5705..9550
                     /gene="entF"
                     /locus_tag="KJL99_003699"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HBD4438280.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAL
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWVEQRRQLPPPASLSPAPLAGR
                     SASADILRLKMEFTDGEFRQLATQLSGVQRTDLALALTALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLATRLAAQLKKMRRHQRYDAEQ
                     IVRDSGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHG
                     DLSIEILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQIN
                     ATQVEIPETTLSALVAEQAAKTPDAPALADARYLFSYREMHEQVVALANLLRERGVKP
                     GDSVAVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQ
                     LPRFSDVPNLTNLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAI
                     VNRLLWIQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAM
                     QQFFAEYGVTTTHFVPSMLAAFVASLTPQTARQSCVTLKQVFCSGEALPADLCREWQQ
                     LTGAPLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPV
                     PPGVAGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEY
                     LGRSDDQLKIRGQRIELGEIDRVMQALPDVKQAVTHACVINQAAATGGDARQLVGYLV
                     SQSGLPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQAP
                     GRAPKAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTP
                     GQVMVASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVL
                     SRYLDPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGIAARLRARGEQV
                     AFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGSTSTELFTTIEGN
                     YADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAELDIYRQDCAHVDI
                     ISPGAFVKIGPIIRATLNR"
BASE COUNT         2105 a         2505 c         2630 g         2332 t
ORIGIN      
        1 cagtaatata agcgaactga aggatgctgt tacggaatat attgaatact ataacagcag
       61 aagaattagc ctgaaattaa aaggtctgac tccaattgaa tatcggaatc agacctatat
      121 gcctcgtgta taactgtcca actttttggg gtcagtacac cctggggctg ccactccaaa
      181 ccagacaatt tggatggtag tcccttcttc gcatggaggt aatataaaca tgctgacgaa
      241 atatgccctt gtggcagtca tagtgctgtg tttgacggta ctgggattta cgcttctggc
      301 cggagactcg ctgtgtgagt ttacggtgaa ggaacgtaat attgagttca gggctgttct
      361 cgcttacgaa ccgaagaagt agccgttgtg cggggagtaa tccccgcata tccggttgtc
      421 aggtcaggat ggtaaggcac ctgctttaca ctttcgcccg cggtcagtga tggctgcggg
      481 cgatttcgtt ttaagagcaa tgatgagttt tgccgacaga agtccgaaac aggactcact
      541 gtataaataa acagctattt tgctaaggaa gggtaagata acggcgggtg cctgaagctt
      601 tccggtttca ggttaactct gaggtttgga aagatgaagc cccaagagat atttctatca
      661 atcctggggc tgccactccg aacctcaaca actcggatgg tagtcccttc ttcgcatgga
      721 ggcaatataa acatgctgac gaaatatgcc cttgtggcag tcatagtgct gtgtttgacg
      781 gtgctgggat ttacgcttct ggtcggagac tcgctgtgtg agttcaccgt aaaggaacgt
      841 aatattgagt ttaaggctgt tcttgcttac gaaccgaaga agtagccgtt gtgcggggag
      901 taatccccgc atatccggtt gtcaggtcag agtggtaagg cacctgcttt acactttcgc
      961 ccgcagtcag tgatggctgc gggcgaatcg taccagatgt tgtcaattaa tcgtgttggc
     1021 acagagttat gactattttt tcttttatct gccagtgcac agcaaacatc tcattctcac
     1081 gatgaatgat gacctgctgt ttattccagc taattatctg atagtccaga aaacctgcat
     1141 cagtttggat ctcacttgcc ttaaatgcgc tctctttggc ggaaaatgca agtgtcagcg
     1201 ccagagaaaa ggctaaaccg cagtctgcga gtcgctcgtg ttctgctggt gtaataatgt
     1261 tgtctgtcaa ttcggtcgct gtttgcgcag aaaaaatttc ttcgatatca acgccaatcg
     1321 gttgacggga aaccacggcc aatgccgtag ccccgcagtg gctaatactg ccgtatacct
     1381 ccgcaggcca caccggttgg cgtagctcgc cgattgcggg cacacattta tagccatatt
     1441 cccgcaaggc ataaacagca gcgatccgtc cggctaaatg ctctgtttta cgtttacgtc
     1501 cagcgtgttg cagttgtgcg tagtgcggca gccagagtaa atcctgctca caaaaattcg
     1561 ccggatcgaa ctcaacaaaa tgcagcgtat gtccggcaaa ggggagggag gtatgcgtag
     1621 ttttcatatc gaccatattc gagactgatg acaaacgcaa aactgcctga tgcgctacgc
     1681 ttatcaggcc tacgtgatcc tgcaatatat tgaattggca agatctttgt aggccggata
     1741 aggcgttcac gccgcatccg gcgtgaacga cgcgcacttt gtcaacaatc tgacgttagc
     1801 atcagaagtg agtattaatg ctcatatacc acgtacgtcc cggctcgtta taggtatacg
     1861 cgccggcacc ggcgatatag ttggctcctg ccagatcgcc cgtggtctgg gcattacctg
     1921 cacgccacaa acgtttgtcg aacaggttgt ccacgccgcc ggtcagactg acattcttcg
     1981 tcacatccca ggtcgcgctc agacccacaa tgctgtaagg actgatttct ttggtttccg
     2041 ggccaaccgc tggctgacct ttatagttgt acttcttcgg ctgctgcttg ccgtaccagg
     2101 tgaaggtcgt ttgcatcgac aaatcttccc gtgcctgcca gctcagcgtt gagttcaacg
     2161 tgtactccgg gatgatcgac aaacggtcgc ccgtggtttt gttttcactc ttcagcatat
     2221 aagtgatgtt attggtccac atcaccgttt cgctaactgg tacgtttaac gatccttcca
     2281 gaccttcaac cactgctttc ggcacgttat cccactgata gagatcagtg ccgactgcgt
     2341 tttgccctac agccacatag cccgcttcaa tcttattgcg ataatcgtta cggaaccagg
     2401 tgacgcccgc cagccagccg tcgcgtttga actccagacc aatctctttg ttgatgctgg
     2461 tttctacttt caggtcatcg ttaccttgca gatagcagcc gcccgcgctg gcatagcagc
     2521 cctgaccttt actgtagaga atgtagttcg ggttagtctg gtataggctc ggcgctttat
     2581 aggcgcgggc aatgcctatt ttcagcgtga agtcatcgcc taaaccttgc gatatgttca
     2641 gcgccgggct ccagttattg ccgacaatac tgtgatgatc gaaacgcagc cccggcgtta
     2701 cgatggtgct gtcagtcagc tccatgttgt tttcggcaaa cagcgagaaa atttctgctt
     2761 ttgaatacgg gctacggtcg gtggcactca cgccatcaat agcgccaccg gtattcgttc
     2821 cggtcagtgc ttgggtgttg gaacttaagt ccttcatccg ttgctgattc cactccgtac
     2881 ccagcgtcag cgtctggtta acgaggaaat caatcggcag gttaacttcg ctgtgcagca
     2941 tcacgtcatc gagatcgata tcgacgaaat cctgtgtcgc tttttcgtta aatttccctt
     3001 cggtaccgcc cgccagacct tccggaatac gcgagttacg ggtgtgttca tactgcaccc
     3061 agttgctggt ggtcacgccg ttatcccagc caccgttcca ggtcagcgcg tagttctggc
     3121 gatacaggcg gttggtttca tcgccatatt tcgagcgagt gtaagcgtca gagttggtgt
     3181 tctgggtatc gcccgcatac aggttaccct ggcggctgta acccgcttcc agttccagcg
     3241 attgcaatgg tgcgaagtcc cagcgcacca cgccgttaat atctttattg atcacccctt
     3301 cgcgcccggc tggtaacgtc gtggcatacg ttccggcacg cgcggactga tggccctggt
     3361 tgatatccca cgcgtcagcc tgagttttgt cgaggttgcc atacaagcgg aagctgaatt
     3421 cgtcgcccag cggaccggtc aggctaaagt tagtgcgttt ggtggcacct tcctctttat
     3481 gttctggcgc attgaaatat gcgtcccagg agccgtgcca ctcgccgctg ccttttttgg
     3541 taatgatgtt aaccacgccg cccgccgcgc cgttgccata acgcgcagct gccggaccac
     3601 gcagaacttc aatacgttca atcatttcag gtggcaccca ggaggtatca ccacgggtat
     3661 cgcgctcgcc acgccagccc tgacgcaccg agttacggct gcttaccggc ttgccgtcaa
     3721 tcaaaatcag cgtgttttcc ggacccatac cgcgaatatc aatctgtcgg ttattcccac
     3781 gctgaccact ggtggagtta ccggtcaggt taacgcctgg catggtacgg atgatctccg
     3841 acacatcgcg ggcaaccggg tttttgcgga tttcatctgc ggtgatggtc gaaacgccag
     3901 gagcctgcaa gttctgctcg gcggcggtaa cgacaatggt atcgtcatgt gaaacaggtg
     3961 tatcggtcgg ctcttgtgcc tgcgctaccc cataaatccc cagattgacc aacaaggcca
     4021 gggaatgaat cttcttgttc attgttttat tcctgcattt ttgccacgaa ttgcaactgt
     4081 cgggcatggt cgtcatcaac acgacgcatc ccgttaccgc gaaaaccttt gatcctgaaa
     4141 gacacgcagt gcagttggtt aattaatgtc cgcgcttccc acggcgcgcc attacgctat
     4201 tgcaaatgca aatagttatt aataacatta tcaatatatt tctgcaatca atgaaaaatt
     4261 gcacagtaaa catggggtta tggtgtgacg gcgttaaaag taggaagtga gagctggtgg
     4321 cagtcgaaac atggcccgga atggcagcgt ctgaatgacg aaatgtttga ggtcactttc
     4381 tggtggcgtg atccccaagg ttctgaagaa tactcgacga taaagcgcgt atgggtctac
     4441 atcactggtg tgaccgatca tcatcagaac agccagcccc agtcgatgca gcgaattgca
     4501 ggtactgatg tctggcagtg gacgacacaa ctcaatgcca actggcgcgg cagctactgc
     4561 tttattccca ccgaacgcga tgacattttt tctgcaccat cccccgatcg cctcgaatta
     4621 cgtgaaggct ggcgaaaact attaccccag gcaatagccg atccgctgaa tccacaaagc
     4681 tggaaaggcg ggctagggca cgctgtttct gcactcgaaa tgccgcaagc gcctctgcaa
     4741 ccgggatggg attgtccgca agcgccagaa ataccagcca aagaaattat ctggaaaagt
     4801 gaacggttga aaaattcacg tcgtgtatgg atttttacca ccggcgatgt aacagcagaa
     4861 gaacgcccgc tggcagtttt gctcgatggc gaattttggg cgcaaagtat gcccgtctgg
     4921 cctgtgctga cttcgctgac ccatcgtcag caacttcctc ccgccgtgta tgtgttgatc
     4981 gacgctatcg acaccacgca ccgcgcccac gaactgccgt gtaatgcgga tttctggctg
     5041 gcagtacagc aagagttatt acccctggtg aaagttattg ccccttttag cgatcgcgcc
     5101 gatcgcaccg tggtcgccgg gcagagtttt ggtgggctgt ccgcgctgta tgccggactg
     5161 cactggcctg aacgctttgg ctgtgtatta agccagtcag gatcgtactg gtggccgcat
     5221 cggggcgggc agcaagaggg cgtgttactt gaaaagctaa aagctggtga agttagcgcc
     5281 gaaggtctgc gcattgtgct ggaagcgggt attcgcgagc cgatgatcat gcgggccaat
     5341 caggcgctgt atgcgcaatt acaccctata aaagaatcca ttttctggcg tcaggttgac
     5401 ggcggacatg atgcgctttg ttggcgcggt ggcttgatgc aggggttaat cgacctctgg
     5461 caaccacttt tccatgacag gagttgaata tggcattcag taatcccttc gatgatccac
     5521 agggagcgtt ttacattttg cgcaatgcgc aggggcaatt cagtctgtgg ccgcaacaat
     5581 gcgccttacc ggcaggctgg gacattgtgt gtcagccgca gtcacaagag tcctgccagc
     5641 agtggcttga agcccactgg cgtactctga caccggcgaa tttcacccag ttgcaggagg
     5701 cacaatgagc cagcatttac ctttggtcgc cgcacagccc ggcatctgga tggcagaaaa
     5761 actgtcggat ttaccctccg cctggagcgt ggcgcattac gttgagttaa ccggagaggt
     5821 tgatgcgcca ttactggccc gcgcggtggt tgccggatta gcgcaagcag atactttgcg
     5881 gatgcgtttt acggaagata acggcgaagt ctggcaatgg gtcgatgatg cgctgatatt
     5941 cgaactgcca gaaattatcg acctgcgaac caatattgat ccgcacggta ctgcgcaggc
     6001 attaatgctg gcggatttgc aacaagattt gcgcgtcgat agcggtaaac cactggtctt
     6061 tcatcaactg atacaggtgg cagataaccg ctggtactgg tatcagcgtt atcaccattt
     6121 actggtcgat ggcttcagtt ttccggccat tacccgccag atcgccaaca tttactgcgc
     6181 attgctgcgt ggcgaaccaa cgcctgcttc gccgtttacg cctttcgctg atgtagtgga
     6241 agagtaccag caataccgcg aaagcgaagc ctggcagcgt gatgcggcat tctgggtgga
     6301 acagcgtcgc caactgccac cgcccgcgtc actttctccg gcacctttag cggggcgcag
     6361 cgcctcggca gatattttgc gtctgaaaat ggaatttacc gacggggaat tccgccagct
     6421 ggctacgcaa ctttcaggtg tgcagcgtac cgatttagcc cttgcgctga cggctttgtg
     6481 gctggggcga ttgtgcaatc gcatggacta cgccgccgga tttatcttta tgcgtcgact
     6541 gggctcggcg gcgctgacgg ctaccggacc cgtgctcaac gttttgccgc tgggtattca
     6601 tattgcggcg caagaaacgc tgccggaact ggcaacccga ctggcaaccc gactggcagc
     6661 acaactgaaa aaaatgcgtc gtcatcaacg ttacgatgcc gaacaaattg tccgtgacag
     6721 cgggcgagcg gcaggtgatg aaccgctgtt tggtccggta ctcaatatta aggtatttga
     6781 ttaccaactg gatattcctg gtgttcaggc gcaaacccat accctggcaa ccggtccggt
     6841 taatgacctt gaactggccc tgttcccgga tgaacacggt gatttgagta ttgagatcct
     6901 cgccaataaa cagcgttacg atgagccaac gttaatccag catgctgaac gcctgaaaat
     6961 gctgattgcc cagttcgccg cggatccggc gctgttgtgc ggcgatgtcg atattatgct
     7021 gccaggtgag tatgcgcagc tggcgcagat caacgccact caggttgaga ttccagaaac
     7081 cacgctgagc gcgctggtgg cagaacaagc ggcaaaaaca ccggatgctc cggcgctggc
     7141 agatgcgcgt tacctgttca gctatcggga aatgcacgag caggtggtgg cgctggcgaa
     7201 tctgctgcgt gagcgcggcg ttaaaccagg ggacagcgtg gcggtggcac taccgcgctc
     7261 ggtctttttg accctggcac tccatgcaat agttgaagct ggcgcggcct ggctaccgct
     7321 ggataccggc tatccggacg atcgcctgaa aatgatgctg gaagatgcgc gtccgtcgct
     7381 gttaattacc accgacgatc aactgccgcg ctttagcgat gttcccaatt taaccaacct
     7441 ttgctataac gccccgctta ccccccaggg cagtgcgccg ctgcaacttt cacaaccgca
     7501 ccacacggct tatatcatct ttacctctgg ctccaccggc aggccgaaag gggtaatggt
     7561 cgggcagacg gctatcgtca accgcctgtt gtggattcaa aatcattatc cgctcacagg
     7621 cgaagatgtc gttgcccaaa aaacgccgtg cagttttgat gtctcggtgt gggagttttt
     7681 ctggccgttt attgccgggg ctaaactggt gatggctgaa ccggaagcgc accgcgaccc
     7741 gctcgctatg cagcaattct ttgccgaata tggcgtaacg accacgcact ttgtgccgtc
     7801 gatgctggca gcatttgttg cctcgctgac tccgcaaacc gctcgccaga gttgcgtgac
     7861 gttgaaacag gttttctgta gtggtgaggc cttaccggct gatttatgcc gcgaatggca
     7921 acagttaacc ggcgcgccgt tgcataacct gtatggcccg acggaagcgg cggtagatgt
     7981 cagctggtat ccggcttttg gcgaggaact ggcacaggtg cgcggcagca gtgtgccgat
     8041 tggttatccg gtatggaata cgggtctgcg tattcttgat gcgatgatgc atccggtgcc
     8101 gccgggtgtg gcgggtgatc tctatctcac cggtattcaa ctggcgcagg ggtatcttgg
     8161 acgacccgat ctgaccgcca gccgctttat tgccgatcct tttgctcctg gtgaacggat
     8221 gtatcgtacc ggagacgttg cccgctggct ggataacggc gcggtggagt acctcgggcg
     8281 cagtgacgat cagctaaaaa ttcgcgggca gcgtatcgaa ctgggcgaaa tcgatcgcgt
     8341 gatgcaggcg ctgccggatg tcaaacaagc cgttacccac gcctgtgtga ttaaccaggc
     8401 ggcagccact ggtggtgatg cgcgtcagtt ggtgggctat ctggtatcgc aatcgggcct
     8461 gccgttggat accagcgcat tgcaggcgca gcttcgcgaa acattgccgc cgcatatggt
     8521 gccagtcgtt ctgctgcaac tgccgcagtt gccacttagc gccaacggca agctggatcg
     8581 caaagcctta ccgttgcctg aactgaaggc acaagcgcca gggcgtgcgc cgaaagcggg
     8641 cagtgaaacg attatcgccg cggcattcgc gtcgttgctg ggttgtgacg tgcaggatgc
     8701 cgatgctgat ttctttgcgc tgggcggtca ttcgctactg gcaatgaaac tggcagcgca
     8761 gttaagtcga cagtttgctc gtcaggtgac gccggggcag gtgatggtcg cgtcaaccgt
     8821 cgccaaactg gcaacgatta ttgatggtga agaggacagc tcccggcgca tgggattcga
     8881 aaccattctg ccgttgcgtg aaggtaatgg cccgacgctg ttttgtttcc atccggcgtc
     8941 cggttttgcc tggcagttta gcgtgctctc gcgttatctc gatccacaat ggtcgattat
     9001 tggcattcag tcgccgcgcc ctcatggacc catgcagaca gcgacaaacc tggatgaagt
     9061 ctgcgaagcg catctggcaa cgctacttga gcaacaaccg cacggcattg cggcgcggct
     9121 gcgtgcccgt ggcgaacagg tggcatttct tggtttgctg gatacctggc cgccagagac
     9181 gcaaaactgg caggaaaaag aggctaatgg tctggacccg gaagtgctgg cggagattaa
     9241 ccgcgaacgc gaggccttcc tggcggcaca gcagggaagt acttcaacgg agttgtttac
     9301 caccattgaa ggcaactacg ctgatgctgt gcgcctgctg acgactgctc atagcgtacc
     9361 gtttgatggt aaagcgacgc tgtttgttgc tgaacgcacg cttcaggaag gtatgagccc
     9421 cgaacgcgcc tggtcgccgt ggattgcgga gctggatatc tatcgtcagg attgtgcgca
     9481 tgtggatatt atctctccag gggcgtttgt aaaaattggg ccgattattc gcgcaacgct
     9541 aaacaggtaa attaatatta tctataaacc ca
//
LOCUS       DAEBXN010000143         9512 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.143, whole genome shotgun sequence.
ACCESSION   DAEBXN010000143 DAEBXN010000000
VERSION     DAEBXN010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9512
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.143"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..276
                     /locus_tag="KJL99_003700"
     CDS             <1..276
                     /locus_tag="KJL99_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HBD4438281.1"
                     /translation="PAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEATDIV
                     VMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
     gene            432..1967
                     /gene="gadC"
                     /locus_tag="KJL99_003701"
     CDS             432..1967
                     /gene="gadC"
                     /locus_tag="KJL99_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HBD4438282.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            2098..3417
                     /locus_tag="KJL99_003702"
     CDS             2098..3417
                     /locus_tag="KJL99_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HBD4438283.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3715..3756
                     /locus_tag="KJL99_003703"
     CDS             3715..3756
                     /locus_tag="KJL99_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438284.1"
                     /translation="MVQLVDLARCVSF"
     gene            3794..4939
                     /gene="dosC"
                     /locus_tag="KJL99_003704"
     CDS             3794..4939
                     /gene="dosC"
                     /locus_tag="KJL99_003704"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HBD4438285.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIALAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKVLLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRILNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSFLSQKRLVKSKILGAGSSRKLAVS"
     gene            4864..5481
                     /locus_tag="KJL99_003705"
                     /pseudo
     CDS             4864..5481
                     /locus_tag="KJL99_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            5739..6071
                     /gene="ddpX"
                     /locus_tag="KJL99_003706"
                     /pseudo
     CDS             5739..6071
                     /gene="ddpX"
                     /locus_tag="KJL99_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
     gene            6085..6981
                     /locus_tag="KJL99_003707"
     CDS             6085..6981
                     /locus_tag="KJL99_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HBD4438286.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLMR
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTSV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            6978..7952
                     /locus_tag="KJL99_003708"
     CDS             6978..7952
                     /locus_tag="KJL99_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBD4438287.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIAPAFSCEPQLIIAGEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCALTACGDNNQHCTCWYPQQEVISV"
     gene            7945..8871
                     /locus_tag="KJL99_003709"
                     /pseudo
     CDS             7945..8871
                     /locus_tag="KJL99_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(8927..9358)
                     /gene="osmC"
                     /locus_tag="KJL99_003710"
     CDS             complement(8927..9358)
                     /gene="osmC"
                     /locus_tag="KJL99_003710"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HBD4438288.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         2320 a         2331 c         2389 g         2472 t
ORIGIN      
        1 ccggcggttt gcttcaaact gaaagatggt gaagatccgg gatacaccct gtacgacctc
       61 tctgaacgtc tgcgtctgcg cggctggcag gttccggcct tcactctcgg cggtgaagcc
      121 accgacatcg tggtgatgcg cattatgtgt cgtcgcggct tcgaaatgga ctttgctgaa
      181 ctgttgctgg aagactacaa agcctccctg aaatatctca gcgatcaccc gaaactgcag
      241 ggtattgccc agcagaacag ctttaaacat acctgataac gtttaacggt aacggtgtcc
      301 cgaaacgaac ccgtttcggg ataatttcca aagtctgttc actggcatta gcaacggaaa
      361 atattgttct gaatacgctt cagaacaaaa caggtgcggt tccgacagga ataccgtttt
      421 agggggataa tatggctaca tcagtacaga caggtaaagc taagcagctc acattactcg
      481 gattctttgc cataacggca tcgatggtaa tggctgttta tgaataccct accttcgcaa
      541 catcgggctt ttcattagtc ttcttcctgc tattaggcgg gattttatgg tttattcccg
      601 tgggactttg tgctgcggaa atggccaccg tcgacggctg ggaagaaggt ggtgtcttcg
      661 cctgggtatc aaatactctg ggtccgagat ggggatttgc agcgatctca tttggctatc
      721 tgcaaatcgc cattggtttt attccgatgc tctatttcgt gttaggggca ctctcctaca
      781 tcctgaaatg gccagcgctg aatgaagacc ccattaccaa aactattgca gcactcatca
      841 ttctttgggc gctggcatta acgcagtttg gtggcacgaa atacacggcg cgaattgcta
      901 aagttggctt cttcgccggt atcctgttac ctgcatttat tttgatcgca ttagcggcta
      961 tttacctgca ctccggtgcc cccgttgcta tcgaaatgga ttcgaagacc ttcttccctg
     1021 acttctctaa agtgggcaca ctggttgtat ttgttgcctt cattttgagt tatatgggcg
     1081 tagaagcttc cgcaactcac gtcaatgaaa tgagcaaccc cgggcgcgac tatccactgg
     1141 ctatgttact gctgatggtg gcggcaatct gcttaagctc tgttggcggt ttgtctattg
     1201 cgatggtcat tccgggtaat gaaatcaacc tctccgcagg ggtaatgcaa acctttaccg
     1261 ttctgatgtc ccatgtggca ccggaaattg agtggacggt tcgcgtgatc tccgcactgc
     1321 tgttgctggg tgttctggcg gaaatcgcct cctggattgt tggtccttct cgcgggatgt
     1381 atgtcacagc gcagaaaaac ctgctgccag cggcattcgc taaaatgaac aaaaatggcg
     1441 taccggtaac gctggtcatt tcgcagctgg tgattacttc tatcgcgttg atcatcctca
     1501 ccaataccgg tggcggtaac aacatgtcct tcctgatcgc actggcgctg acggtggtga
     1561 tttatctgtg tgcttatttc atgctgttta ttggctacat tgtgttggtt cttaaacatc
     1621 ctgacttaaa acgcacattt aatatccctg gtggtaaagg ggtgaaactg gtcgtggcaa
     1681 ttgtcggtct gctgacttca attatggcgt ttattgtttc cttcctgccg ccggataaca
     1741 tccagggtga ttctaccgat atgtatgttg aattactggt tgttagtttc ctggtggtac
     1801 ttgccctgcc ctttattctc tatgctgttc atgatcgtaa aggcaaagcg aataccggcg
     1861 tcactctgga gccaatcaac agtcagaacg caccaaaagg tcacttcttc ctgcacccgc
     1921 gtgcacgttc accacactat attgtgatga atgacaagaa acactaacca tgtctgaatg
     1981 gttataagac aagggagcga taattcatcg ctcccttttt cgtgcttgct gtcgtattga
     2041 cttaaccggg ataagtacga gaatgagcgc acatctgttt accggaaacc agcacatatg
     2101 gatatctgct cccgaaacaa gaaattaacg attagaagac cagcgatact ggttgcattg
     2161 gcacttttac tgtgtagttg taaaagcacg cctccagagt ccatggtgac accaccagca
     2221 ggttcaaagc caccagccac gacgcaacaa tcgtcacaac cgatgcgtgg catctggctg
     2281 gccacggttt ctcgcctcga ctggccaccg gtttcctcgg ttaacattag taatcccacc
     2341 agccgagccc gtgtacaaca acaggcgatg atcgacaaac tggatcatct ccaacgtctc
     2401 ggcataaaca cggtcttttt ccaggtcaag ccggacggta ccgccctgtg gccatcgaaa
     2461 attttgccgt ggtccgatct tatgaccggt aagattggtg aaaatccggg ttacgatccg
     2521 ctgcaattca tgctcgacga agcccacaag cgtgggatga aagtacacgc ctggtttaac
     2581 ccctatcgcg tatcggttaa tacgaagccc ggtactatca gggaactgaa tagcactctg
     2641 tctcaacaac cggcgagcgt ctatgtgcaa caccgtgact ggatcagaac gtctggcgat
     2701 cgctttgtcc tcgacccggg catccctgag gttcaggact ggatcacatc aatagtcgct
     2761 gaagtggttt cccgctatcc ggtagatggc gtgcagtttg acgactattt ctatactgaa
     2821 tcaccgggtt cacggctaaa tgataacgaa acgtaccgta aatacggagg cgcatttgcg
     2881 tcaaaagcag actggcggcg caacaatact cagcagttaa ttgcaaaggt atcacacacc
     2941 attaaaagca ttaagccggg agtcgaattt ggcgttagcc cggcaggcgt gtggcgtaac
     3001 cgatcacacg atccgctcgg ttccgatacc cgaggcgcgg cagcctatga cgaatcctac
     3061 gcagacaccc gtcgatgggt ggaacaagga ttgctggatt acattgctcc ccaaatttac
     3121 tggccgttct cacggagtgc cgcgcgttat gacgtgttgg caaaatggtg ggcggatgtc
     3181 gttaaaccaa ccaggacccg cctgtatatc ggtatcgcct tctataaagt gggtgaacct
     3241 tcaaagatag agccagactg gatgattaac ggcggcgtac cggaactgaa aaagcagctc
     3301 gatcttaacg atgcggtacc agaaattagc ggcaccatct tgttccgtga ggactatctg
     3361 aataaaccgc agactcaaca agcggtcagc tatctgcaaa gtcgttgggg cagttaaact
     3421 accatgctta ctttgtaaca agccggtgct ttacccaccg gcttgttaca ccttgtgaaa
     3481 tatcccacta gtaatcatgc ttacataagt caaattaacc acgcaattaa ccacacttag
     3541 ttaaacagct ataaatccct aataaaaatg tatgaatatg aaaaaccata cgatattcgc
     3601 tgtcaccgaa aaataattac gatgggctat actcttctga agaacagtag cgtagattga
     3661 aactgtctta agtcgcttat ttcttttcag tatatcttca tatttcagga gaatatggtg
     3721 cagttagtcg atctcgcacg ttgcgtttcg ttttgattcc gtctgatttc ccagccttat
     3781 aagggtgtga attatggaga tgtattttaa aagaatgaaa gatgagtgga ccggacttgt
     3841 cgaacaggca gatcctctca ttcgtgctaa agccgcagaa attgcccttg cgcatgctca
     3901 ttatctgagt atcgagtttt atcgaattgt ccgcatcgac ccgcatgccg aagaattctt
     3961 gagtaatgaa caagttgagc ggcagttgaa aagtgcgatg gaacgctgga ttattaacgt
     4021 gctttctgcc caggttgacg atgtcgaaag gctaatacaa atccagcata ccgtcgcgga
     4081 agtgcatgcc cgcataggaa ttccggtaga aattgtcgag atggggtttc gggtgctgaa
     4141 aaaaatcctc tatccggtaa tcttctcttc ggattattcc gccgcagaaa aacttcaggt
     4201 ctaccatttc tcgattaaca gtattgatat cgcgatggaa gtgatgaccc gcgcgtttac
     4261 ctttagtgac agtagtgcct caaaggaaga tgaaaactat cgtatcttct cgttactgga
     4321 aaacgccgaa gaagaaaaag aacggcaaat agcctcaata ctttcatggg aaatagatat
     4381 tatctataaa gtcctgctgg attctgattt aggcagcagt ttgcctttaa gccaggctga
     4441 ttttggcctg tggtttaacc ataaaggtcg acattatttt agcggcattg ccgaagtggg
     4501 ccatatctcc cgtctgattc aggatttcga cggtattttc aatcaaacca tgcgtaacac
     4561 caggattttg aataacagaa gtttgcgggt gaaatttcta ttacagataa gaaataccgt
     4621 atcgcagatt attaccttgc tgcgtgaatt gtttgaagaa gtatcgcgcc acgaagtcgg
     4681 tatggatgta ctgacgaaat tacttaaccg ccgtttcctg ccgactatct tcaaacgcga
     4741 aattgcccat gccaaccgga ccggtacacc gctgtcagtg ctgattattg acgttgataa
     4801 attcaaagag atcaacgata cgtggggcca taacactggc gatgaaattc tgcgtaaagt
     4861 ctcattcctc tcgcagaaga gattggtgaa atcgaaaata ttgggcgctg ggtcatcgcg
     4921 gaagcttgcc gtcagttagc agaatggcgt agccagaata ttcatatccc ggcgttatcc
     4981 gtgaacttgt cggcgctgca ctttcgcagt aatcagctgc ctaatcaggt gtctgatgca
     5041 atgcaagcct ggggtattga cggccaccag ctgacggtgg aaatcacgga aagcatgatg
     5101 atggaacacg ataccgaaat ctttaagcgc attcagatcc tgcgtgatat gggcgtgggt
     5161 ttatcggtag atgattttgg cacgggcttt tccggattat cccgcttagt cagtcttccg
     5221 gtaacggaaa tcaaaattga caaaagcttt gtcgatcgtt gtctgacgga aaaacgcatc
     5281 ctggccttac ttgaagccat taccagcatt gggcaaagcc tcaatttaac cgtcgtggcg
     5341 gaaggggtcg aaaccaaaga gcaatttgag atgctacgta agatccactg tcgcgttatt
     5401 cagggatatt tcttttcccg cccccttccc gccgaagaaa ttccaggctg gatgagcagc
     5461 gtgttaccgc tgaaaatctg acaaattcct ctcgcccgca ctcgcgggtt ttcctttaag
     5521 gtgacactgt cacttaaata ctgtgatttc agctacgatt ccgggagatt ccttccttaa
     5581 ctttccctct tgcaccaaaa ctgcactacg ctgcacaaat gttgatcaaa agttaaaaaa
     5641 tataacttta agccagcatt ctggcgctta tcccggcatg gcatgagatc tgcataagcg
     5701 gaaagcgcag caatttttgt cttatacagg aaccgtttat gtcggatacc accgaactgg
     5761 ttgatttagc cgtgatcttc cctgatctgg agatcgaatt gaaatatgcc tgcgttgata
     5821 acatcacagg taaagctatt tatcagcaag cgcgttgtct gctacataaa gatgcgatta
     5881 ccgcgctggc gaaaagtatc agtatcgccc agctgtcagg cttacaactg gtgatttacg
     5941 atgcgtatcg cccacaacaa gcacaggcga tgttgtggca agcctgccca gacccgcaat
     6001 atgttgttga tgtgacggtc ggttctaatc acagccgtgg cacggcgctc gacctgacgc
     6061 ttcgtgatga cgtggaggtg gtgaatgatg ctaagcgagg aaacgtccgc cgtacgccca
     6121 caaaaacaaa cgcgatttaa cggtgcaaaa ctggtatgga tgctgaaagg cagtccgctc
     6181 accgtgaccg gcgcagtcat cattgtatta atgcgactga tgatgatttt ttcaccgtgg
     6241 ctggcgacgc atgatcccaa cgccattgat ttaaccgccc gccttttgcc gccttctgcg
     6301 gcgcactggt ttggcaccga tgaagtggga cgcgatctgt ttagccgcgt actggtcggc
     6361 agtcagcaat caattctcgc cggattagtg gtggtcgcca ttgcgggtat gattggttcg
     6421 ctactcggat gtctatccgg tgtgcttggc ggacgcgcag acgccatcat catgcgcatc
     6481 atggacatta tgctgtcgat tccttcgttg gtactgacaa tggcactggc tgccgctctc
     6541 gggccgagtt tatttaacgc catgctggcg attgctattg tgcgaattcc cttttatgtg
     6601 cgcttggcgc gggggcaagc attagttgta cgccagtata cctatgttca ggcggcgaaa
     6661 acctttggcg cgtctcgttg gcatctgatc aactggcata ttttacgtaa ctccctaccg
     6721 ccgctgatcg tgcaggcatc gctggatatc ggtagcgcga ttttaatggc cgccacgttg
     6781 ggatttattg gcctgggtgc tcaacaaccg agtgctgaat ggggcgcgat ggtagcgaat
     6841 ggtcgcaact atgtactcga tcagtggtgg tattgcgcat ttccgggggc agcgattttg
     6901 cttacctccg tcgggtttaa tctctttggt gatggtattc gcgatctgct tgacccgaaa
     6961 gcaggaggaa agcagtcatg acccaacccg ttctggacat tcaacagctg catttgagtt
     7021 tccccggttt taacggtgat gttcacgcgc tcaacaatgt gtccttgcag attaaccgcg
     7081 gcgaaattgt cggtctggtg ggagaatccg gctcaggtaa atcagtcacc gcaatgctga
     7141 ttatgcgtct gctaccgacg ggcagttatt gcgtacatcg ggggcagatt tcactgctgg
     7201 gagaagatgt tcttaacgct cgggaaaaac agcttcgtca gtggcgcggc gcacgagtgg
     7261 cgatgatctt tcaggaaccg atgaccgccc ttaatccgac acgtcgaata ggtcttcaga
     7321 tgatggacgt aatccgccat catcaaccaa taagtcgtcg ggaagccaga gctaaagcga
     7381 ttgcactgct ggaagagatg caaatcccgg atgccgtgga agttatgtcg cgctatccgt
     7441 ttgagctttc aggtggtatg cgccagcggg taatgattgc gccggcattc tcctgcgagc
     7501 cgcaattgat tattgccggc gaaccgacga cggcgctgga cgtcacagta cagttgcagg
     7561 tactgcgttt gcttaaacat aaagcacgcg ccagtggaac tgcggtactg ttcatcagcc
     7621 atgatatggc cgtggtgtcg caactgtgcg atagcgttta cgtgatgtat gccggaagcg
     7681 taattgaaag cggcgggacg gcagacgtta tccatcatcc ccggcatccg tataccattg
     7741 gtttgctgca atgcgcacca gaacatggag taccacgcca gccattaccc gccattccag
     7801 ggacggtacc aaacctcacc catttgcctg acggctgcgc ttttcgcgat cgttgctatg
     7861 cggcaggtgc acagtgtgcg ctgacagcgt gtggtgacaa caaccagcac tgcacctgtt
     7921 ggtatccgca gcaggaggtc ataagtgtct gacacgttat taacgttacg cgacgtccat
     7981 atcaattttc cggcccgtaa aaactggctt ggcaaatcta cggaacatgt tcatgccatt
     8041 aatggtattg atttacagat ccgccgtggt gaaaccttag ggatcgtcgg cgagtcaggc
     8101 tgcggcaaaa gcaccctcgc acagctttta atgggcatgc tgcaaccgag ccacgggcag
     8161 tacatccgtt caggctcaca acgcattatg cagatggtgt ttcaggaccc gctctcttcg
     8221 cttaatccgc gcttaccggt gtggcgcatc atcacagaat cgctctggat agctaagcgt
     8281 agtagtgaac aacagcggcg agcgttggca gaggagttgg ctgtgcaggt gggtattcgt
     8341 ccggagtatc tcgaccgcct gcctcatgcg ttctccggcg ggcagcggca acgcatcgcc
     8401 attgccagag cactctcttc gcagcctgac gtgattgtgc ttgatgagcc aacctcggcg
     8461 ctggatatct ccgtgcaggc gcagatcctc aatttgctgg taacgctaca ggaaaatcac
     8521 gggctgactt atgtgctgat ttcacacaat gtctcggtga tacgtcatat gagcgatcgg
     8581 gtggcggtga tgtatctcgg gcagattgta gaactgggcg acgcgcaaca ggtgttgact
     8641 gcttcggcac atccatacac ccgattattg ctggattccc tccccgccat tgataaaccg
     8701 ctggaggaag aatgagcatt acgtaaaacg gatctgccgg gaaaccgcac gttgccgcaa
     8761 ggctgttttt tctacgaacg ttgccctctg gcaacccacg gatgtgaagt ccggcaatca
     8821 ttagcaataa gggaggacgg acgtgacctc cggtgctggc gggcgctgta gagatgctaa
     8881 caacgcatca ggcattcacg cgcctgacgc gtcatccggc aatgctttac gatttcaact
     8941 ggtaatccag cgtaatttcc gctttcagta cctgagagac cgggcatcct gcttttgctt
     9001 tctggatgat gccgtcaaag gtagaggcat caatacccgg caccgcaact tcactcttca
     9061 gtgcgatttt cgtaatcgca aaaccggcat ccactttatc cagcgacaca tcggcggtgg
     9121 tatcaattga tgttggcgtg aatcccgctt cccccagcat taatgaaagc gccattgaga
     9181 aacatgcggc atgcgctgcg ccaatcagtt cttcagggtt ggttcctttt tcgccttcaa
     9241 aacgcgtgtt aaatccatac ggctgttggt tcagcacgcc gctctcggtg gatactgttc
     9301 ccttcccgcg tttgatatcg ccttcccagt gtgcctgacc tttcttatgg attgtcattg
     9361 ttgctctcct gtgggcttaa aaaggaaatt atagactacg tgagaaacag cacgaggata
     9421 agcaggatat tccgaataaa atacggcgaa aatattactt ctgcacttaa attaaattcc
     9481 cacaatagat aaccgggaat atatatctca ag
//
LOCUS       DAEBXN010000144         9494 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.144, whole genome shotgun sequence.
ACCESSION   DAEBXN010000144 DAEBXN010000000
VERSION     DAEBXN010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9494
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.144"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(<1..190)
                     /locus_tag="KJL99_003711"
     CDS             complement(<1..190)
                     /locus_tag="KJL99_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HBD4438289.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPTCGVVCDPYIC
                     VNSDGISPELTRKYLGEKA"
     gene            710..1405
                     /gene="minC"
                     /locus_tag="KJL99_003712"
     CDS             710..1405
                     /gene="minC"
                     /locus_tag="KJL99_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HBD4438290.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALKDKIAQAPTFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTMPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            1429..2241
                     /gene="minD"
                     /locus_tag="KJL99_003713"
     CDS             1429..2241
                     /gene="minD"
                     /locus_tag="KJL99_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HBD4438291.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            2245..2511
                     /gene="minE"
                     /locus_tag="KJL99_003714"
     CDS             2245..2511
                     /gene="minE"
                     /locus_tag="KJL99_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HBD4438292.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2883..3227)
                     /locus_tag="KJL99_003715"
                     /pseudo
     CDS             complement(2883..3227)
                     /locus_tag="KJL99_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3262..3381)
                     /locus_tag="KJL99_003716"
     CDS             complement(3262..3381)
                     /locus_tag="KJL99_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBD4438293.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(3342..3527)
                     /locus_tag="KJL99_003717"
     CDS             complement(3342..3527)
                     /locus_tag="KJL99_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438294.1"
                     /translation="MSRRYSSATITLVILFFRRPHCLKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            3628..3801
                     /locus_tag="KJL99_003718"
     CDS             3628..3801
                     /locus_tag="KJL99_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438295.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3803..4147
                     /locus_tag="KJL99_003719"
     CDS             3803..4147
                     /locus_tag="KJL99_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HBD4438296.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVSAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            4151..4486
                     /locus_tag="KJL99_003720"
     CDS             4151..4486
                     /locus_tag="KJL99_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HBD4438297.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4543..6864)
                     /locus_tag="KJL99_003721"
     CDS             complement(4543..6864)
                     /locus_tag="KJL99_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBD4438298.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSACACVSSLDEESTADTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKDETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELDDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGGDLLTGGFTDSDSWRLGVMAGYARDYNLTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDISKKGAYIDSWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGVSNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(6981..7190)
                     /locus_tag="KJL99_003722"
     CDS             complement(6981..7190)
                     /locus_tag="KJL99_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438299.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVVS"
     gene            complement(7941..9464)
                     /locus_tag="KJL99_003723"
     CDS             complement(7941..9464)
                     /locus_tag="KJL99_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBD4438300.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTADVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIASAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLGYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
BASE COUNT         2659 a         2205 c         2080 g         2550 t
ORIGIN      
        1 cggctttttc gccgagatat ttccttgtta actctgggga aatgccatct gagttcacac
       61 aaatatacgg gtcgcaaaca acgccgcacg tcggagattg aagtccgcca gcgtcatgtc
      121 cagacgccat tgatgagaac atcatcccca cacctgacaa tccgatataa ataatacgca
      181 tcatattcat agcacatcct gataatccaa ccgtacaaca cttatgctca tttcgtcatc
      241 aatccgtaat caaaaccgct gacctggacg agttacgatg acctgacaga agtatcagac
      301 gtcttctgcg gtttctatga tagagcaatt ctgtggcgga tattaagata acgggtatct
      361 gttctttaat gatgctgcaa aaaatggaaa gttaagaaag cctggcgtgg tgaaaaaata
      421 cacttgcctc aatactggat atttaaacag gtctggagat gctgtcacga ttagggtgcg
      481 gaaaaaggaa gcgtaaataa gaagaatcaa aagtacgacg gcaatgggtt gattgacaag
      541 ggtatttttt aagctatgaa tcagcgccat ttatcacaga atagactttt actctgaata
      601 aatgggaggg tgacttgcct caatataatc cagactataa catgccttat agtcttcgga
      661 acatcatcgc gcgctggcga tgattaatag ctaattgagt aaggccagga tgtcaaacac
      721 tccaatcgag cttaaaggca gtagcttcac tttatctgtg gttcatctgc atgaggcaga
      781 acctaaggtt atccatcagg cgctgaaaga caaaatcgct caggccccca catttttaaa
      841 acatgccccc gttgtactca acgtcagtgc actggaagac ccggtaaact ggtcagcgat
      901 gcataaggcg gtttcggcaa ccggtttgcg ggttattggc gtaagtggct gcaaagatgc
      961 gcaacttaaa gccgaaattg aaaaaatggg gctgcctatc ctgacggaag gaaaagaaaa
     1021 agcgccacgt ccagctcccg caccgcaggc tccagcgcaa aatacaatgc cggtcacaaa
     1081 aacgcgttta atagataccc cggtgcgttc cggtcagcgt atttatgctc cacaatgtga
     1141 tctgattgtt acaagccacg ttagcgctgg ggccgaattg attgccgatg ggaacattca
     1201 tgtctatggc atgatgcgcg gtcgtgcgct ggcaggggca agtggtgacc gggaaacgca
     1261 aatattttgt acgaacctga tggcggaatt ggtgtccatc gcaggtgaat actggctgag
     1321 tgatcaaatc ccagcagaat tttatggcaa agcggcgcga ctgcagttag tcgaaaacgc
     1381 tttgaccgtt caaccgttaa attgatccct ttttaacaag gaatttctat ggcacgcatt
     1441 attgttgtta cttcgggtaa agggggtgtt ggtaagacaa cctccagcgc ggccatcgcc
     1501 actggtttgg cccagaaggg aaagaaaact gtcgtgatag attttgatat cggcctgcgt
     1561 aatctcgacc tgattatggg ttgcgaacgc cgggtcgttt acgatttcgt caacgtcatt
     1621 cagggcgatg caacgctaaa tcaggcgtta attaaagata agcgtactga aaatctctat
     1681 attcttccgg catcgcaaac acgcgataaa gatgccctca cccgtgaagg ggtcgccaaa
     1741 gttcttgatg atctgaaagc gatggatttt gaatttatcg tttgtgactc cccggcaggg
     1801 attgaaaccg gtgcgttaat ggcactctat tttgcagacg aagccattat taccaccaac
     1861 ccggaagtct cctcagtacg cgactctgac cgtattttag gcattctggc gtcaaaatca
     1921 cgccgcgcag aaaatggcga agagcctatt aaagagcacc tgctgttaac gcgctataac
     1981 ccaggccgcg taagcagagg tgacatgctg agcatggaag atgtgctgga gatcctgcgc
     2041 atcaaactcg tcggcgtgat cccagaggat caatcagtat tgcgcgcctc taaccagggt
     2101 gaaccggtca ttctcgacat taacgccgat gcgggtaaag cctacgcaga taccgtagaa
     2161 cgtctgttgg gagaagaacg tcctttccgc ttcattgaag aagagaagaa aggcttcctc
     2221 aaacgcttgt tcggaggata agttatggca ttactcgatt tctttctctc gcggaagaag
     2281 aacacagcca acattgcaaa agaacggctg cagattattg ttgctgaacg ccgtcgcagc
     2341 gatgcagaac cgcattatct gccgcagttg cgtaaagata ttcttgaggt catttgtaaa
     2401 tacgtacaaa ttgatcctga gatggtaacc gtacagcttg agcaaaaaga tggcgatatt
     2461 tctattcttg agctgaacgt gaccttaccg gaagcagaag agctgaaata agcccgctgt
     2521 aaaaacgcat ttatcttcaa ggcagagtca tttctgcctt gagtttttca tccctctcat
     2581 ccacgttgtg gtaaagcggc gagtattctt gctgatactc ctcattgcta tttccacctc
     2641 cccctttaca ccttaaggct gttcccacgc acagaacaaa atgtttatgc ctggttgagt
     2701 aaataaccat gttattagtg tgtatatact gggagatgat aaccccgcaa tttcattatt
     2761 cattgttctc ccataggatg aggtttcccg ccgtaactgg cgggcttttt ttgccccaga
     2821 attttctctt tcaacatcct gtaaacgaaa actgcgccga agcgcagttt aatcgttgat
     2881 ggtcagaacg accagttcac accagccacc gcgttccacg gggactccac accggcacca
     2941 tggctatacc ccaccccgaa atgcccgctt aacgtactgc tgaatgaggc tttaatacct
     3001 gcctggtata ttccacgtct gcccgacaaa tcattgacaa aattaccgtc actattcact
     3061 ttcacccgat tatcatcgac aaattctttg cgcacagccg ccttcaggca gggttcaatt
     3121 tccataccgt tccccagacg catgttgtaa ctcagcgttg cacccagttc acgatatata
     3181 ctgcgggtat cgactgattt cgattccatg ccattggata aatgatagta gcgtctaagt
     3241 gacctaagct ctgctgcaac atcagcccgc aggcaccaga ccaggggatt catcctgaag
     3301 agacagcgca ggtgtattgt gttcaccgct caccaaagac atcatgatga aatgatgata
     3361 tcccgcataa gaaagaggca ttttttacac gcagtgcgct gaagtgtagt tggataaaaa
     3421 atcactcttt tcagataata ctgtcgtacg cttatcttta gacaatgagg ccgtcggaaa
     3481 aataaaatga ctaatgtaat cgttgctgat gaatatcttc tgctcacata aaaatcacac
     3541 aataatattg ataaatcaga ttatcttacc tttacaacat tgcgttaaca gtcaatgaca
     3601 cttcttgaat aaaaatattg tcaatcaatg agttattcct catttaagat tattctcatt
     3661 cacgtaaaaa acataattcc tatcatcaca gccacgctca ttttgaatta tctgaataat
     3721 agcgagcgtt ctttagttaa gcagatctta attgaagacg aaattattgt gtgtgcaact
     3781 tacttaatac cggacatcta agttgaagaa aaaaatactt gccttcgggc ttatctcagc
     3841 attgttctgc tccacgccag cgatggcgga tatgaaccgc accacgaaag gtgcattgct
     3901 aggcgcaggc gttggtttac ttactggcaa tggtgttaat ggcgtactga aaggtgcagc
     3961 tgttggcgct ggtgttagtg cagtaacaga aaaaggccgc gacggtaaaa atgcgcgtaa
     4021 aggtgcaaaa gtgggtgccg ccgttggtgc tgtcactggc gttctgaccg gcaatggcct
     4081 cgaaggggcg attaaaggtg ccgtgattgg cggtacgggt ggtgcaattc tgggtaaaat
     4141 gaaataagga atgatgatga aaaaatttgc tttactggcg ggcctgtttg ttttcgcacc
     4201 aatgacctgg gcgcaggact acaatattaa aaacggtttg ccgtcagaaa cctatattac
     4261 ttgcgcagaa gccaacgaaa tggcaaaaac tgacagcgca caggttgcag aaattgttgc
     4321 ggtaatgggt aatgccagcg ttgccagccg tgatttaaaa attgagcaat ctccggaact
     4381 gagcgccaaa gtggtagaaa aactgaatca ggtttgcgct aaagacccgc aaatgctgtt
     4441 gatcacggca attgatgaca ccatgcgtgc tatcggcaaa aaataatatt tcatatgtac
     4501 cgcgtcgatg aatacgacgc ggttttttag ctaacggcgg gatcagaact tatatttcag
     4561 gcccaccatc actgcggtgt cattgtagcc attatcaccc agctgcacgc ccacattccc
     4621 ccagacgctg gccgctggat tcaggttgcc atttacgcca agtttaattt cccccaaatt
     4681 actcacccca tcttgttcga ctttcacacc gttcattgag acagcaaaat ctttactgtt
     4741 atggatccag ttcatttcaa caaaaggttc aaagtcgtca ccgtgcggac cgctgttttt
     4801 ctcctgagta cgaataaagg tgcggaagcc gagtcgggtc tggatgttgt tatttgcgtc
     4861 attctcaata cgtgagccat tggcttccgt gtgcgcatta tgatcgacgc ccatccagat
     4921 agcctgtgcc tgtggctgga agatccacgt atatttcgcc gcttccagac caaaggattt
     4981 attcagggca aaaccgtaac ccgcttccag cgagacggtt gcacctttcg cgctatagga
     5041 ttcataggct aattcatccc ctttcacgga gtttttaaac cagctatatt gcgcccagga
     5101 gtcaatgtat gcgccttttt tactgatgtc atcggcaaac caagtggcat acagccctgc
     5161 gctatagcct ctgacactcc ctttcgaacg ataatccgac acgctggaat gagttaagtt
     5221 gtagtcgcgg gcataaccag ccatcactcc caaacgccaa ctgtcactat cggtaaaacc
     5281 accggttaac agatcgcccc ctaactgcga gacgtagcga tgcgaggtcg ttctcaactg
     5341 tccgttgctg tcacgccagg cattatgccc gccaatttga cgtagccaaa gtcggcttga
     5401 acgctcctgt tcagttacag gatcgatgta gcgcgtttca cccgcacggt cgttcagacg
     5461 cataacaaaa agagtgttag cggctgccag gttggcgacg taacttccag cttcggggcg
     5521 caggacgggg gtaggcgcaa cagtaggcgc tgaacttcct ccggagtttg tttcaccgcc
     5581 gctcgttgcc tggaagttag tcagatacca gtttttattg gtcgcttcgg tattacctcg
     5641 ggttaatgaa tattcgtagg caccggcgaa aatcctcgaa tccttaataa attccccatt
     5701 tgattccccc tcaacgctga taatctctat cccatttaac gtgtaagctc cgctgcctcc
     5761 tgcattggta actttaactc gggttgttcc agaggtattt ccattaataa cgactttatc
     5821 cgtagctgaa ttatcatcat ccaattcagt tctgagatgc aatacgccat tattaccaac
     5881 gtaattttca gttattgtta atcttgttgg ttcgacatct cgtccatctg cccgggaaat
     5941 atagaccgta ctattatcaa ccgtgaggtt gctcaccgtt gagtcagctt taatatccca
     6001 ggtactatta tctgaaagcg acagataggt atgagtgtta tcatctgttg aaatattagc
     6061 tgaacccgtt atttttgaat tagttgcatc gacggtcaaa tgacctttaa tattattgat
     6121 aaaaacatct gcagccgatg atacctcact atccgtaatg gtcacgttac tcgtctcatc
     6181 tttatggtag aggatttcat ttgctgaccg tagtgagcta ccagataaaa tatctaccgt
     6241 tgcttcctga ccatttatgg aagactccag cacaattcca gacccctgat gcgcgactgc
     6301 agatgtattt ttcagcgtca ccgtgccgcc attataaatt ttagcaatcg cggcgtcagg
     6361 acccgttgta taaatttctc cctggttaaa tgaaactgtc ccctggcggg cagcgatggc
     6421 atgagcattg tccccatgcg tggtgatatc aacatcggtt aatgtaacgc tggcattggt
     6481 ataatcaagc gtgtcaccag ccttgacgac agtgccatta gcgtacgcgg cataactttc
     6541 tgttccgtgg gttgtgatct ccccttttgc catatccagt tttgctatgg cattattttc
     6601 attacttcct gtatcggcag tactttcttc atcaagagaa gatacacacg cacacgcgct
     6661 tccgggagat gcacacgcaa gaacgccgac tgcgtgatta cccgcagtaa caataaatgt
     6721 atcatctccg tcagaaaaag agggggtaac cgaaccgtca gagttgactc ttattatggt
     6781 gtcagtattc aggtttccac tcgtttgact tagcaaacca attgagtaat cccccgttgt
     6841 gacaatcgtg tttttaccct gcaaaataac agcaccgcca aattcactta cgattgcgct
     6901 agctgttaat gctgggctta gaggactatt gtcagaagga tataaaccgc ccgttgttac
     6961 aatgacgcca ttgttaatgg ttaacttact accctctcct gcaacggata cccgtaactt
     7021 ccattacccg ttgttataac tagcgcatta tcaatatctt ttacttttcc atcttttatt
     7081 gccacagcat gtttatattc atcgtgctga ataaactgtt cagcatatgc attacccacc
     7141 gccagagcaa taagtcccaa actaaacccg gggagttttt tcagtttcat agattaacta
     7201 tccattcatt ggattaacaa acattcctgg tcaaataacc tatgagctca gattaccaaa
     7261 acggagggta aagacaaaaa caaaagcaca aaaaatttaa atagataaac agcgacaata
     7321 tataaatgat aaataagtcg gcattatcat ttttcacctt attttcatga cattgatcac
     7381 tttggggtga ctattttgtt taacaaataa ctcttcgaca tcaaaattat taatagttgc
     7441 catggtaatt atttcggtga tatataaaaa caactgtgat aacaccaagg ttgacaacta
     7501 tttgcgcaat aattcctttc cttcaatgat ccaggcgttc actgccagca acccattcag
     7561 ccgcatactg acagtgagaa actgaaagat tattgctgat ccctgtggct aacgagtctt
     7621 tctcttaaat cgattaacca gattgatcgc cagccaaaca agggcattaa gcaaatttat
     7681 cgtcacaagc acttctccca gcccggtact gggttttgca ataaggatga ccaggagggt
     7741 gatcagcata aatgcaatgg aaaagatgat atagagagtg aaataatcca gattattact
     7801 attattcatg ctactaatgg caatcctgtc aaaactttca aacaattata gcaaacaaaa
     7861 taatgaacaa tagtaacaag aggcaacgag ctaattagct gtctataatt acaccgcact
     7921 atggccttac tgatcaattt tcactcaacc acaaccttca ccttcggttt agaaaggata
     7981 attttgacca ggtcgatgta tgtaacaggt ttatagaaat aataaccctg ctgaaatgtg
     8041 atattatttt ggttcagata gccaagttgt tctttcgttt cgacaccttc agcgacgata
     8101 ctgatcgaaa gtttacgcgc cagatccaat acacaatcca gaattcgggt tgattctggg
     8161 tctgcgttta cgcggccaac gaaactgtga tcaattttga tataatcaat atgcaggtca
     8221 tgaagataag aaagccctga gtagccagta ccgaaatcat ccagcgcgat gacaaaacca
     8281 ttttcatgca gaatgttcaa ccgctgaacc agactttcat caacattcaa tggctcacgc
     8341 tcggtgactt ccagaacaag gtttaaatcg cggcgggtaa aactgtcacg gaaatttaaa
     8401 cactcgtcga caaacgtcgg cgaaataata tgcgaagcgc taaaattaat tccaatatga
     8461 aaaccttccg gcagtttact cgcgatagcg ttcatctgtc tggcaacctg attcatcagg
     8521 ctttgtgtaa gcggaacgat taatccggat ttttcagcaa gtggaataaa tgccgcgggt
     8581 gaaatatatc caccgtgagg ttgtttccag cgggctaaca cctcaactcc ccgcaatgtc
     8641 ccttcccgac catttaccac aggttggtaa aaagggacga tctccccatt atttattgcc
     8701 cgtcgcagga tctcttcagg cgttgcgctt ttattgaaat atctatcaag caggaaggca
     8761 gcggcgctgg cgattaacag aataaatatt aaaatcccaa agcctttatc gataagtctg
     8821 tagaagctaa agagtggcgg cgggttaaag ataatagtga aatgatattt tgtggagtga
     8881 accttctcgg tatagggata atgtccatta agcgtcatca catcagccgt tggtccaata
     8941 atgtcatccg ccacgcgtaa tacatacctt acccctttca agggtacatt aagcgccccg
     9001 cgaatatgtt gatcacttat cgtaaccaaa atgcgcgtat ctgcaaattg gctctgatag
     9061 agcaggatag gtaatctatt aacggtgtcg attgccggag ccaacagtaa attactgtcc
     9121 gggacaacag gaatacgaga cagcaggacc cgattcccag gcagggatgt acaccagact
     9181 attccctgtt tgagaatgat gattgtgcgc aggtgaggtt tcagagctgc ttcagtgcca
     9241 agctgatatt gctcctcgag gtcgcattcc ttcccggcaa tgttaaccgc tgtttgagtc
     9301 gcctgtgacg cttcttcaag tatatgattg atattgttgg cggcatatct tgctcccgcc
     9361 aggtactctg cttttgcgga ataccagagc tggatgttca aaatagcgac accggtaata
     9421 aacaagcata tcgcaacgag gatgggtatg agtgtattgc gcatttgctt gtcccggcca
     9481 aagggtttaa taca
//
LOCUS       DAEBXN010000145         9482 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.145, whole genome shotgun sequence.
ACCESSION   DAEBXN010000145 DAEBXN010000000
VERSION     DAEBXN010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9482
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.145"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(8..328)
                     /gene="psiF"
                     /locus_tag="KJL99_003724"
     CDS             complement(8..328)
                     /gene="psiF"
                     /locus_tag="KJL99_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HBD4438301.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQALKGDDRNKFMSACL
                     KKAA"
     gene            complement(447..1862)
                     /gene="phoA"
                     /locus_tag="KJL99_003725"
     CDS             complement(447..1862)
                     /gene="phoA"
                     /locus_tag="KJL99_003725"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HBD4438302.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTDVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTYTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(1963..2223)
                     /gene="iraP"
                     /locus_tag="KJL99_003726"
     CDS             complement(1963..2223)
                     /gene="iraP"
                     /locus_tag="KJL99_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HBD4438303.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPCQ"
     gene            complement(2405..2608)
                     /locus_tag="KJL99_003727"
     CDS             complement(2405..2608)
                     /locus_tag="KJL99_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438304.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFIAFIYNKG"
     gene            2686..3666
                     /gene="ddlA"
                     /locus_tag="KJL99_003728"
     CDS             2686..3666
                     /gene="ddlA"
                     /locus_tag="KJL99_003728"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBD4438305.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINTLPGFTNISMYPKLWQASGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(3690..3902)
                     /locus_tag="KJL99_003729"
     CDS             complement(3690..3902)
                     /locus_tag="KJL99_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HBD4438306.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGVLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            4162..4470
                     /locus_tag="KJL99_003730"
     CDS             4162..4470
                     /locus_tag="KJL99_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HBD4438307.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLAGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(4529..5623)
                     /gene="yaiW"
                     /locus_tag="KJL99_003731"
     CDS             complement(4529..5623)
                     /gene="yaiW"
                     /locus_tag="KJL99_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HBD4438308.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVYSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(5636..6856)
                     /gene="sbmA"
                     /locus_tag="KJL99_003732"
     CDS             complement(5636..6856)
                     /gene="sbmA"
                     /locus_tag="KJL99_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HBD4438309.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            7208..8365
                     /gene="ampH"
                     /locus_tag="KJL99_003733"
     CDS             7208..8365
                     /gene="ampH"
                     /locus_tag="KJL99_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HBD4438310.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITHPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPRIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(8366..8869)
                     /locus_tag="KJL99_003734"
     CDS             complement(8366..8869)
                     /locus_tag="KJL99_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBD4438311.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     RATLLSMIDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAI
                     NRLPSEY"
     gene            complement(8957..>9482)
                     /locus_tag="KJL99_003735"
     CDS             complement(8957..>9482)
                     /locus_tag="KJL99_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBD4438312.1"
                     /translation="LSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKTGYDFKLGDA
                     GYVTPYGSISGLFQSGDDYQLSNDMKVDGQPYDSMRYELGVDAGYTFTYSEDQALTPY
                     FKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDV
                     DQDWSANVGVKYTW"
BASE COUNT         2352 a         2424 c         2249 g         2457 t
ORIGIN      
        1 tcaggtatca ggcggctttc ttgaggcagg cactcataaa cttattacga tcatcacctt
       61 tcagagcttg ttgcgttgct tgattattac attcacgcat cttttgctgc tgtggcgtca
      121 aacttttttc accaggcgca gacttgctgt tcttcaggca atcactcatg taggtcttac
      181 gagcatcccc tttcaacgcc tgcgccgtcg cctgctgatt acaggaggtc atacgctgtt
      241 gttgtggggt taaggttctc tcggcagcgc cgacggtggt taaaaaaacc agaccaaaaa
      301 gcaaggtaac cagtaatgtt attttcatag caccatccct cttcatgttt taaccatgag
      361 cgtttgcgcc cgtgatctgc cattaagtct ggttgctaac agcaaaaaaa ccacccggca
      421 gcgaaaattc actgccgggc gcagttttat ttcagcccca gagcggcttt catggtgtag
      481 aagagatcgg tctggtcggt cagtccaaca acattggcgg catgcgggcc atacgccgca
      541 atacgcaact gactgccggt atgttcttgt gaatcctctt cggaattccc gtaactcatc
      601 accatcactg cgccatcttt agtatttagc gcctgagtga ggcccggcgc tttggtatcc
      661 ggcgcaacaa cctggctggc gtgggcgtga tcagcggtga ctatgaccag cgtgttaccc
      721 tcctttttag cgaattccag cgcccgttgt acggcttcat cgagatcgac cgtctcgcca
      781 atttgcccac aagggttcgc agcgtgatcc tgtttatcga ttgacgcacc ttcaacttgc
      841 aggaaaaatc ctttctcatt tttactcaac aattcaatgg ctttgtcggt catctgtgcc
      901 agggttggta cactgtcatt acgttgcgga ttaggcgtat aggtgactgc gggcttatcg
      961 atattgccgt ggtacgttgc tttcggccct agccagcgca ctggcatatt gccgtcagca
     1021 aacaggccaa gcaggggttt ttgctgattc gcttccgtca ccgaattcag tgaggcagca
     1081 tcgttcacca actgataacc acgcgcctgt gcctgttcac gcagcgtttt tccctgccat
     1141 tcaccagcgg ttgccgtttc agcaaaggtt tttgcgccgc cgccaagcgt aacgtcggca
     1201 cgagcgttaa gcagctgttc ggtaatcgat ccttttccgc ctttttccag agcgttaccc
     1261 ggacattttt cactggtcgc gctcggaccg tagcatttgc gcgaagtcac atgtgccacc
     1321 agcgcagcgg gcgtggcatc ctgcaactct gcggtagaaa cgttaccggt cgccagacct
     1381 gcggcttttg ccatttccag aatcgttggg tgatcttttt cgtgaatatc gacgcccagc
     1441 gcgccgttat aggttttgac atcggttgac caggcggttg ctgatgcagc cgagtcggtg
     1501 acgtagtccg gtttgccagt ttttttattc agcgcatagt gagtgtattg cccggtaagc
     1561 ggtaaggcat cgataccttt aaaaaagccg cccgcacctt cggcataatt acgtgcggca
     1621 gtaatttccg aatcccccat cccatcgcca atcagcaaaa taatattttt tgcaggttta
     1681 tcgctaagag aatcacgcag agcggcggtc tgatcacccg ttaagcggcg agcaccgccg
     1741 ggtgcagtaa tatcgccctg agcagcccgg ttttccagaa caggcatttc tggtgtccgg
     1801 gcttttgtca caggggtaaa cagtaacggt aagagtgcca gtgcaatagt gctttgtttc
     1861 actttatttt ctccatgtac aaatacatta aaaaataaaa acaaagcgac tataagtctc
     1921 ggccgtgaca actttatgac agctgttgaa aagattaact ttttactgac aaggatgctt
     1981 caataacttc tttacgtaat cgcgcagcag ctctgtatcg tcgtcaggaa tgctggcatc
     2041 gggctttacc tcgtacagcg ccccctctac ctgatcaatc aaccgctgtt ggtcattttg
     2101 cgccatattg cgaagcattg cagtgacgat aatctccaaa gcttctacct gcgcacacag
     2161 ttctttcgac tcttcttctt tttgggcaag cttaaataac aactcagcaa tgagattttt
     2221 catgtctgta tttccttatc caaagtatgg agaagttaac attatgtttc acaacttcat
     2281 agtgtctgga agtattattt ttatgacaaa ggaaatactt tgcgcaaata ttttttgttt
     2341 gattgccagc gaaacgtttc tcttatttaa ataattaccc acacaaaata taggctttta
     2401 attatcaacc tttattataa ataaaagcta tgaaatatat tctcttcgtt aacttataat
     2461 taagagaaaa aaactcaccg cattgccatt taagccagga ataaattagc ttcccgttca
     2521 tcatttttgt gatcttgcgc acatctttga gataacgcac tttcttcacc agtacgttgc
     2581 acgctttgtc agcagaagat tgcaacaatc tttcagaagg ccgcgaatcc cccgttttgt
     2641 cgtggtaatg tatgcacttt gtgttagacg ggataggttt ttaagatgga aaaactgcgg
     2701 gtaggaatcg tttttggtgg taaatcagcg gaacatgaag tgtctctgca atcggcaaaa
     2761 aacattgtcg atgccattga taaaagtcgc ttcgacgttg tgctgctggg cattgataaa
     2821 caagggcaat ggcacgtcag cgatgccagc aattatctgc taaatgcaga cgatcctgcc
     2881 catattgcgt tgcgcccttc ggcgaccagc cttgcgcagg tgccaggtaa acatgagcat
     2941 cagcttatcg acgcgcaaaa cggtcagccg ttgccgacgg tggatgtcat tttcccgatt
     3001 gtccacggta cgctgggcga agatggttcc ttgcagggaa tgctgcgggt cgccaattta
     3061 ccgtttgtag gttctgatgt tctggcttca gcagcctgta tggataaaga tgtcaccaaa
     3121 cgtctgctac gcgatgccgg gctgaacatt gcgccattta ttaccctgac gcgcgctaat
     3181 cgtcacaaca tcagttttgc cgaagtggag tctaaactgg ggttaccact gtttgtaaaa
     3241 ccggctaatc agggctcttc tgttggtgtc agcaaagtaa ccagtgaaga acagtacgca
     3301 attgccgtcg atctggcgtt cgagttcgat cataaagtga tcgttgagca agggatcaaa
     3361 ggtcgtgaga tcgaatgcgc agttctgggc aacgacaatc cgcaagccag cacctgtggc
     3421 gagatcgtac tcaccagcga tttctatgcc tacgacacca agtacattga cgaagatggc
     3481 gcgaaagtgg tagttccggc agccattgca ccagagatca acacactgcc tggcttcacc
     3541 aacatcagta tgtatccgaa gctgtggcaa gccagcggtc tgggttacac cgatctgatc
     3601 acacgtttga ttgagctggc gctggagcgt cacgctgcgg ataacgcatt gaaaaccaca
     3661 atgtaatatt ttaacgcccg ataatattct tattgggcgt tttcagtttc ttcatcgcga
     3721 cgacgaataa tcaaccccgc cagccagaaa ctaatcaccc acgtcaccag accgacggca
     3781 taggtctgcc aaccttttgc ttcaaatccc agtaccccca ccacgccatt aagaatgaat
     3841 ataaggccaa tggcgaacgc atagtagtgc cagtcacggc ggatttttac aggcagattc
     3901 atggcagctc cgttaacgaa aaacaacatc cttgcatatt ctgtaacaac cgtctgtggt
     3961 gtagacaaca ttttcgtaat tattaataaa tttaacttaa ggttgccatt ttgtgactca
     4021 caacgaagat tcacgcttcg ggaatattcc caacatcaat ttaccatgat tctgatattg
     4081 acaatctaaa tgacctgtca gacttgccat aagccgctgg tatcacacca gaagagaagc
     4141 agatgacttc cggaggtttt tatggctgat ttcaccctgt caaaatcgct gtttagcgga
     4201 aaatatcgca atgcctcttc aacgcctggc aacattgcct atgcgttgtt tgtgctgttt
     4261 tgcttttggg ctggggcgca attgctgaac ctgttagtgc atgcgcccgg cgtctatgag
     4321 cgtttaatgc aggtccagga aacaggtcgc ccacgggtgg aaattggttt aggtgtcggc
     4381 accattttcg ggctgatccc gtttttagca ggctgcctca tttttgcagt ggtggcgcta
     4441 tggctgcact ggcgacatcg ccgccagtaa aatttacgtt gccagatgcg gcctgaaacg
     4501 ccttatctgg cctgacattc ctgtttgatc actgtttcat acaacgcgcc cgccgttcgt
     4561 ctacgcgctt cgcaaaccag gccgtagtca ggttgcgggt gattttcggg ctttccagtt
     4621 gaatgccagg caacatttcg cggggtagtg atttacccgt tttcgcttcg gcaagttgat
     4681 aaactttctt gtacagcgcc gtctcctcaa aagagaagct gtcacctttc tctaactgac
     4741 ggcgaatttc gctgtcgctc atccccagtt ttcccgccag tttgcgcgtt gccagttcgg
     4801 ttttcccggg ttctttactg tcgtaacgaa ttaaatcacc gtccagtgcc agcttcacgc
     4861 cgctggcctt actgactgcg ttctgaaacg cggcattacg gctggcgtac cagccagcgt
     4921 taaaatcagc aaagcggtat atcggtgcgc tatagctggc gggataattc agtaaatggt
     4981 aagtaccaaa ccacaacccg ccgcggcgac tgaagacttc ctgacgaact gtaccgtcca
     5041 ttttccacgg ataccctttg gtatgctgtt cggcaaaagc aatgctgacc tgcatcggcc
     5101 caccggtgcg caccggatta agcgaaccaa acagcgtctg ccccattggt accatgctga
     5161 tcagatcgtc aaaaatcgcg ctcaattgct tttctgtacg caccgaatct aaccgttcgc
     5221 tataactttt gccgtttggc gatttgattt ttagcgcggt atgaaccaga aaagcaggaa
     5281 tgtgcatccg ttcagcacga cggtcaatct cttgccaggc gatcttgctt aagcccggaa
     5341 cggccggatc tgcctgataa tttgactcct gctgcgccac cgccagcacc gagtagacgt
     5401 tttccagcgt cggtgccagc ccctgacttt caaaggtggt ggcgagatct ttcgcccagg
     5461 catcgcggtc tttcacgctc gcaggcatct tctgccgcac gacgctcgct acatctatcg
     5521 ctttttcgcc ctttttcaac ggttgcggag cctgactact acaccccgct aaaaccagaa
     5581 ccgccaacaa tgacagcgaa gatagatgat tgacgcgcga cattacttct cctttttagc
     5641 tcaaggtatg ggttacttcc tgaattttgt caccatccag ctcatgttca aagctgcgca
     5701 gacgtttgta gatagacatc aactcaacca gtgtggtcca tgagttaatc aggtactgga
     5761 aagcaccgcg aacctgacca aaaacgttgg taatctgcgt catcaggccg agcgtaatcg
     5821 taccggcaac aattgacgga aacagcaaga acaaaccgaa aacgttatcg acctgcaaat
     5881 agagaatgcg ggcgatgttg aaatacatat agtgaaaata gaggcgaaaa tagtttttcc
     5941 gtacggcgct aaacagctcg cgtaccgtag gcggcgtcgc gcgcgtggca tcgtcttcac
     6001 cataaaccag ctctttacgg taggcggcct ctactcgctg gtttttaaac tccagccccg
     6061 gcagtttgat ccctaccact gccagcaatc cggtccccat cagcgaccag acgatagcgg
     6121 caatcaccag accatacgga atgtgcccga taatcggcag ctccggcaca tgcgcggaga
     6181 gcgttaccag caccggcagg aaggcgatca acgtcatgat ggcgttgata aaactgacgc
     6241 ccatattctc cagcgttgaa gcaaaacgca tggtgtcttc ctgcacacgc tgtgcggcac
     6301 cttcgatatg acgcagttgt tgccagttcg ccatgtaata ttcgttcatc gccgtacgcc
     6361 agcggaacac gtagtgactg acaaagaagt tgttcagtac actgatcacc accgcgatca
     6421 gcgcaatccc cagaaagacg cccacttcgc ggtaaaattg ttcgatagtg actttatgcg
     6481 gcgaacttag cgcggtttga atcagatcat agaacggcgc ataccaggcg ttgacggcga
     6541 ccccgacttc caccaaaaac caagtgacga agataatcag cgcagtaccg agtattgacc
     6601 agtattgcca acggtgcggg ctgtagataa accagaaaaa tgcaaaaagt cctacgcaaa
     6661 caatgtagta agcgtaaaaa atcaggaaat ccaacgacca gaaacgcgcg gcgctaatcg
     6721 ggatctgccc ggaagcgccg gtgatacgtg ccacccagtc acccccaccg gcttgccaga
     6781 agataacggc aatcaatgcc caaacaaagg ccgagagaaa aaacgttccc ggctttggga
     6841 aaaaagactt aaacatatgc actcctgcta acttcttatc gttttaacaa ctgctgtgta
     6901 ttaaccgcat gaccgctaca aagcgtcagc caaatgaaac cgcccattgt gacccatagc
     6961 aaggcgctta gttcgccagc ctgaccataa gaattgtttc gacggatttc acaaaccaaa
     7021 gattgtttac ataagcggat taacacgcgc aggatcacaa atgcaggcat tggtgagcag
     7081 gaaaaagatc gtgtttacgc gccgttacgt ttttaacact attattgcac taattcgccc
     7141 tttgcaatcc atcaatgagt agtataaata cgctcagtta ccttcattca atctatggac
     7201 accaccgttg aaacgtagtc tgcttttttc tgccgtgctg tgtgcggcgt cattgacctc
     7261 cgtccatgcg gcgcagccga tcactgaacc ggagtttgcc tctgatattg tcgatcgtta
     7321 tgccgatcat attttttacg gcagcggtgc cacggggatg gcgctggttg ttatcgacgg
     7381 taaccagcgc gtctttcgta gttatggtga aacgcgacct ggtaataacg ttcgcccgca
     7441 gctggattcg gtcgtgcgta ttgcttccct caccaaacta atgaccagtg aaatgctggt
     7501 gaaattgctc gaccagggga ccgtaaagtt aaacgatccg ctaagtaaat atgctccgcc
     7561 aggcgcacgc gtgccgacct acaatgggac gcccattacc ctagtgaatc tggcaaccca
     7621 taccagcgcc ctgccccgtg aacagcccgg tggcgcggca catcgtccgg tgtttgtctg
     7681 gccaacgcgc gagcaacgct ggaaatacct ttctacggcg aagctgaaag ccgcgccagg
     7741 cagtcaggcg gcgtactcta accttgcgtt cgacctgctg gcagatgcac tggcgaatgc
     7801 gtccggcaag ccttataccc agttgtttga agagcagatt acccatccat tggggatgaa
     7861 agacaccacc tacaccccct caccggatca gtgccgccgt ttaatggttg ctgagcgcgg
     7921 tgccagtccg tgcaataaca cgctggcggc aattggcagc ggcggcgtct attccacgcc
     7981 tggcgatatg atgcgctgga tgcagcagta tttgtcatct gatttttatc agcgcagtaa
     8041 ccaggcagat cgcatgcaga cgttgattta tcagcgcgcg cagtttacga aagtgattgg
     8101 catggatgtc cccggcaaag cggatgcgct tggtttaggc tgggtatata tggccccgaa
     8161 agagggtcgt ccgaggatta ttcagaagac aggcggtggt ggcggtttca ttacctatat
     8221 ggcgatgatc ccgcagaaga atattggcgc gtttgtggtg gtcactcgtt cgccgttgac
     8281 gcgctttaaa aatatgagcg atggcatcaa tgatctggtg accgagctaa gcgggaataa
     8341 accgttggtt atccccgcat cctgattaat actctgaagg caaacggttg atagcgacca
     8401 gtttgccttt attcatttcg atatagccgc ctttacgcaa agcagcgaga acttctgcga
     8461 cgacagaacg cgatatgcgt gtacgttgat ggatataatt catcacacca atacgtgatc
     8521 gcaattcttc attccagtca atcattgata ataatgttgc gcgggtttgt ttggctggta
     8581 gatgatatcc cgtacaattt ccttctgata ttaatttgta tggtatatcg tttttcatta
     8641 aaccatcagc cagccccata atataaggag cctgggtaat accgataagt acgttttctt
     8701 ctctacgcag agagataact ccttctaaaa taacgaaagt atcttcgtta ttgacatcac
     8761 tggaaaatat aacttgcttt tcattattaa actcgaagcg cgtaccgtat ctggacaaac
     8821 atttatcgag cttaccaaat tcctgaagag gtttaactac agataacatt tgcgcgtcct
     8881 ttgcagtaat gcccgtcaaa tccttgacgg gcattattta gattaaatta ccagtatttc
     8941 ttcggagtga agaatattac caagtatatt taacacccac gttcgcggac cagtcttgat
     9001 ctacgtcacc accaccgagg tagttagcat cggtataggc gctgaagttc ttggtgaagc
     9061 tgaactgagt acccagacca acacgtaccg cagacccttc agtaccgtta tcgatggaat
     9121 caccgttcac atcgttatcg ttgttagagt cgtcgtagac gtaagccagt ttgaagtacg
     9181 gagtcagagc ctgatcttcg ctgtaggtga aggtataacc tgcatctaca cccagttcat
     9241 aacgcatgct gtcgtaaggc tgaccgtcaa ctttcatgtc gttgctcagc tggtagtcat
     9301 caccagactg gaacagacca gaaatgctgc cgtaaggagt cacgtaacca gcatcaccca
     9361 gtttgaagtc gtaaccggtt ttcaaaccga agccccaagc gtcggagttg gtgctaccgt
     9421 caacgtaagt accgttgctc atggttgcag acaggtcgtt gttgaagtga gagtagctca
     9481 ag
//
LOCUS       DAEBXN010000146         9381 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.146, whole genome shotgun sequence.
ACCESSION   DAEBXN010000146 DAEBXN010000000
VERSION     DAEBXN010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9381
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.146"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            128..1825
                     /gene="maeA"
                     /locus_tag="KJL99_003736"
     CDS             128..1825
                     /gene="maeA"
                     /locus_tag="KJL99_003736"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HBD4438313.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            1959..2969
                     /gene="adhP"
                     /locus_tag="KJL99_003737"
     CDS             1959..2969
                     /gene="adhP"
                     /locus_tag="KJL99_003737"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HBD4438314.1"
                     /translation="MKTAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMEEECIVVADYAVKVPDGLDSAAPSSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMDADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            3115..3399
                     /locus_tag="KJL99_003738"
     CDS             3115..3399
                     /locus_tag="KJL99_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HBD4438315.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVVVSRLRRLVTQ"
     gene            complement(3627..4280)
                     /gene="fdnI"
                     /locus_tag="KJL99_003739"
     CDS             complement(3627..4280)
                     /gene="fdnI"
                     /locus_tag="KJL99_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HBD4438316.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4273..5157)
                     /gene="fdnH"
                     /locus_tag="KJL99_003740"
     CDS             complement(4273..5157)
                     /gene="fdnH"
                     /locus_tag="KJL99_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HBD4438317.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5170..8217)
                     /gene="fdnG"
                     /locus_tag="KJL99_003741"
     CDS             complement(5170..8217)
                     /gene="fdnG"
                     /locus_tag="KJL99_003741"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(7630..7632),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HBD4438318.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPKYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGILRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETQAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8449..9330
                     /gene="yddG"
                     /locus_tag="KJL99_003742"
     CDS             8449..9330
                     /gene="yddG"
                     /locus_tag="KJL99_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HBD4438319.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEIYLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYTAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         2237 a         2480 c         2388 g         2276 t
ORIGIN      
        1 acgattaaac accattgcct gcgcaatggt gtttttatct gctttatact tggggacgac
       61 gccctggcgg taaagcaaag acgataaaag cgtgccaggg atggatattc aaaaaagagt
      121 gagtgacatg gaaccaaaaa caaaaaaaca gcgttcgctt tatatccctt acgctggccc
      181 tgtactgctg gaatttccgt tgttgaataa aggcagtgcc ttcagcatgg aagaacgccg
      241 taacttcaac ctgctggggt tactgccgga agtggtcgaa accatcgaag aacaagcgga
      301 acgagcatgg atccagtatc agggattcaa aaccgaaatc gacaaacaca tctacctgcg
      361 taacatccag gacaccaacg aaaccctctt ctaccgtctg gtaaacaatc atcttgatga
      421 gatgatgcct gttatttata ccccaaccgt cggcgcagcc tgtgagcgtt tttctgagat
      481 ctaccgccgt tcacgcggcg tgtttatctc ttaccagaac cgccacaata tggacgatat
      541 tctgcaaaac gtgccgaacc ataatattaa agtgattgtg gtgactgacg gtgaacgtat
      601 tctggggctt ggtgaccagg gcatcggcgg gatgggcatt ccgatcggta aactgtcgct
      661 ctataccgcc tgtggcggca tcagcccggc gtataccctt ccggtggtgc tggatgtcgg
      721 aacgaacaac caacagctgc ttaacgatcc gctgtatatg ggctggcgta atccgcgtat
      781 cactgacgat gaatactatg aattcgttga tgaatttatc caggctgtga aacaacgctg
      841 gccggacgtg ctgttgcagt ttgaagactt tgctcaaaaa aatgcgatgc cgttacttaa
      901 ccgctatcgc aatgaaattt gttcttttaa cgatgacatt cagggcactg cggcggtaac
      961 agtcggcaca ctgatcgcag ccagccgcgc ggcaggtggt cagttaagcg agaaaaaaat
     1021 cgtcttcctt ggcgcaggtt cagcgggatg cggcattgcc gaaatgatca tcgcccagac
     1081 ccagcgcgaa ggattaagcg aggaagcagc gcggcagaaa gtctttatgg tcgatcgctt
     1141 tggcctgctg acggacaaga tgccgaacct gctgcctttc cagaccaaac tggtgcagaa
     1201 gcgcgaaaac ctcagtgact gggataccga cagcgatgtg ttgtcactgc tggatgtggt
     1261 gcgcaatgta aaaccagata ttctgattgg cgtctcagga cagaccgggc tgtttacgga
     1321 agagatcatc cgtgagatgc ataaacactg tccgcgtccg atcgtgatgc cgctgtctaa
     1381 cccgacgtct cgcgtggaag ccacaccgca ggacattatc gcctggaccg aaggtaacgc
     1441 gctggtcgcc actggcagcc cgtttaatcc tgtggtatgg aaagataaaa tctaccctat
     1501 cgcccagtgt aacaacgcct ttattttccc gggcatcggc ctgggtgtta ttgcttccgg
     1561 cgcgtcacgt atcaccgatg agatgctgat gtcggcaagt gaaacgcttg ctcagtattc
     1621 gccgctggtc ctgaacggcg aaggtctggt actaccggaa ctgaaagata ttcagaaagt
     1681 ctcccgcgca attgcgtttg cggttggcaa aatggcgcag cagcaaggcg tggcggtcaa
     1741 aacgtctgct gaagctttgc aacaagccat tgacgataat ttctggcaag ccgaataccg
     1801 cgactaccgc cgtacctcca tctaagcctg cacccggtag tgaaggctac cgggctattt
     1861 ccctctccct ttttcagatc tcatccatac tgggtagtgg cgaataaatc tcatttgcct
     1921 cacctgctat gcagaacatc atccgaaaag gaggaactat gaagactgca gttgttacga
     1981 aggatcatca tgttgacgtt acggataaaa cactgcgctc actgaaacat ggcgaagccc
     2041 tgctgaaaat ggagtgttgt ggtgtatgtc ataccgatct tcatgttaag aatggcgatt
     2101 ttggtgacaa aaccggcgta attctgggcc atgaaggcat cggtgtggtg gcagaagttg
     2161 gcccaggtgt cacctcatta aaaccaggcg atcgtgccag cgtggcgtgg ttttacgaag
     2221 gatgcggtca ttgcgaatac tgtaacagtg gtaacgaaac gctctgccgt tcagttaaaa
     2281 atgccggata cagcgttgat ggcgggatgg aggaagagtg catcgtggtc gccgattacg
     2341 cggtaaaagt gccagatggt ctggactcgg cggcgcccag cagcattacc tgtgcggggg
     2401 tcaccaccta caaagccgtt aaactgtcaa aaattcgtcc cggtcagtgg attgctatct
     2461 acggtcttgg cggtctgggt aacctcgccc tgcaatacgc aaagaatgtc tttaacgcca
     2521 aagtgatcgc cattgatgtc aatgatgagc agttaaaact ggcaaccgaa atggatgcag
     2581 atttagcgat taactcacgc accgaagacg ccgccaaaat tgtgcaggag aaaaccggtg
     2641 gcgctcacgc tgcggtggta acagcagtag ctaaagctgc gtttaactcg gcagttgatg
     2701 ctgtccgtgc aggcggtcgt gttgtggctg tcggtctgcc gccggagtct atgagcctgg
     2761 atatcccacg tcttgtgctg gatggcattg aagtggtcgg ttcgctggtc ggcacgcgcc
     2821 aggatctaac cgaagccttc cagtttgccg ccgaaggcaa agtggtgccg aaagtcgccc
     2881 tgcgtccgtt agcggacatc aacaccatct ttaccgagat ggaagaaggc aaaatccgtg
     2941 gccgtatggt gattgatttc cgtcactaag aggtctttgc tgcgaccccc atgttcgggt
     3001 cgcagcatcg cacactctcc aacatgaaat ggctaaatgg attattgttg cgtaggaagg
     3061 aacaataatg atagtgttcc cagagaagta acgctactga gagtaaagac actaatgaaa
     3121 atggccaatc atccccgccc gggggacatt attcaggaat cactggacga acttaatgtc
     3181 agcctgcgcg agtttgccag agcaatggaa attgcgccct caacggcaag ccgattgctg
     3241 accggaaaag cagctttgac gccagaaatg gcaattaaac tttccgtggt gatcggcagt
     3301 tcgccgcaaa tgtggctgaa tctgcaaaac gcctggagtc tggcagaggc agaaaaaacg
     3361 gtggttgtgt cgcgattacg ccgtctggtt acgcaataaa aaatggcacc ctataaagcg
     3421 ccatttgaaa ctgatgacaa acgcaacatt gcctgatgcg ctacgcttat caggcctaca
     3481 cgttcacttc aatctattgt atttgaaggg ttttcatgcc ggataaggca ttcacgacgc
     3541 atccgggatg aacaaagccc acgttgtcta caatatgaaa atggcgcctt ttacagcgcc
     3601 attttcagct taaagttcaa aagagattat atcccttctt cactctcttt tttcgcttct
     3661 gccttctcga tttcacgata ccagcgcgga tggtgtttct tcgcccagcg acggctcacc
     3721 ttcccttcga tcatcccttt aatcgaccct ttcacccaga atgccatata catatggatc
     3781 aggatggcgt ggatcaggat gatacccgca gccgcgtgga tcagcaggct gtagcgaaca
     3841 acctgcatcg ggaagtactg cgcaaagtac ggacgccaga taatcacccc ggtcaccagc
     3901 agcacgaaaa tcatgctcat gatcgaccag aacatcatct tttgcccggc gttgtactta
     3961 ccaacatccg ccactttgtg ctcattgcct tttaatactt cgacaatgtt caacagccac
     4021 ggaatatctt tcttatccgg gatgttgtga tgcacaaaac gcacaaacat aaacatcagt
     4081 gcgacgaaaa tcgcaatgcc gaagaagggg tgcaaaatgc gtcccatctg cggcgtaccg
     4141 aaggtttgcg tcagccattg cagcgtcggg aagaagaacg aaatcccgga cagcgccacc
     4201 aggaagaagc aaatcaccac ggtccagtga caggcgcgat caataaattt ggtgcgcaca
     4261 atcattttcg acttactcat gatgatcctc ctcgtcatcg tccacttcct tattcgggcc
     4321 aataccgatg tagtggaaaa tcaacccggc aaaagtggca ataaagccag ccgctgccag
     4381 cggtttcaac gcgcctttcc acaggcttac cgaggtgtcg atcttcggat ctttcggcag
     4441 accgtgatac agctccggct gatcggcgtg atgcagcacg tacataacgt gcgtaccacc
     4501 gaccccttcc gggttgtaga cgccagcatg ttcgtaacca cgcgctttca gtttcgccac
     4561 gcgctgttcc gccagctcca gcatctcctt cttggtgccg aagtggatag ccccggtcgg
     4621 acaggttttc acacaagccg gttcctggcc gacgctgacg cgatcgacgc agagcgtgca
     4681 tttatatacc cggttatcct ctttgttgag gcgcggaata ttaaacggac acccggcaat
     4741 gcagtaacca cagccgatgc agttttccga ctggaaatcg acaatcccgt tagcgtactg
     4801 aatgattgca ccagcagacg ggcacgcctt caggcagccc ggatcttcac agtgcataca
     4861 gccgtcttta cggatcagcc actccagctt gccattctgt tcggtttcgc taaagcgcat
     4921 caccgtccag gacttggcgc tcagatcggc ggggttatcg taaaccccga cgcagtgccc
     4981 cacttcatca cggatgtcgt tccactccga acacgccacc tgacaggctt tacagccgat
     5041 acaggtggaa acgtcgataa gtttcgcgac ttctgctttg taatcacgca cctgagaagg
     5101 cggcgtgatg gagttagttg cggacctttt gataatatcc tgcgtttcca tagccatctg
     5161 ttcgccccct tacgccttct cgatgttgac taagaacgct ttatattccg gcgtttgcga
     5221 gtttgcatca ccgacattcg gcgtcagagt gttagcgata taacctttac gcgcgacacc
     5281 ctcaaagccc cagtggattg gaatacccac cgtttcaacc tgctgaccgt ttacattcag
     5341 cggtttcaga cggcgcgtta ccactgccac cgcgcggata aagcctcgct tgctggagac
     5401 agtgacacga tcgccattat taatgccttt cgccgccgcc tgcgtttcgc tgatttccac
     5461 aaactgttcc ggctgagcaa ttgcgttgag caacgcgtgc ttggtccagg tgtggaagtg
     5521 ctcggtcaga cgataggtcg tacccacata cgggaactgc tcttttttac ccatccgcag
     5581 cgcgtcttgt tcatacagac gaacaaccgg gttagacacc acgttcggat gcagcgggtt
     5641 agtgcccagc ggcgtttcaa tcggctcgta gtgttccggg aacggacctt ccgccatttt
     5701 gttgatggca aacaggcgtc ccatcccttc cggctgcata ataaacggcc cggttggcgt
     5761 acccggtgcg gcattgccga agtccggaat atcgttaccc gtccacttgc tgccgttcca
     5821 ctggatcagc atccgtttcg gatcccacgg tttaccgttg atatccgccg aagcacggtt
     5881 gtagagtacg cgacggttga gcggccacgc ccaggcccat cccagcgtat tccccagacc
     5941 ggacgggtcg gagttatcgc ggtttgccat ctggttaccc tgctctgtcc agctaccggt
     6001 gtagatccag caagaagatg cggttgtacc gtcatcacgc agatgcgcaa agctactcag
     6061 caactgacct ttcttcgcaa tcagcacgcc attagcatca tagagatctt ccaacgcata
     6121 gccgttgttc tctttagcta cttcgtcaga ttgcggttcg tgcggctgct tgtagttcca
     6181 gctcatcttc atcagcggtt ctacgccttt accaccttcg gcctggtaca actcgcgcag
     6241 atgatggtag atacccgcca gaatttcgcc gtcgttacgc gcttcgcccg gcgcgtcctg
     6301 acctttccag tgccactgca accagcgacc ggagttagcg atagaaccat cctcttcagc
     6361 aaagcaggtc gaaggcagac ggaatacttc agtctgaata gacgccggat cgacatcgtt
     6421 cgactcgccg tggttctgcc agaaggtaga ggtttcagtc accagcggat cgataaccac
     6481 catgtacttc agtttgctca ggcagctcac cactttgttt ttatccggga aggacgcaac
     6541 cgggttaaag ccctggcaga aataaccggt aactttgcct tcatccatca tgttgaaata
     6601 cttgatgacg tcgtaggtct ggtcccactt cggcagccag tcatagcccc agttgttctc
     6661 tttctgcgcg gcatcgccat agaaagattt catcaggcta acgaagaact tcggatagtt
     6721 gctccagtag ttcacctgat cagccagcgt cgctttcggc gtgttcgctt ccagatacga
     6781 ctgcaaatca acctgttttt ctgacggcag cgtcagataa cctggcaggc tggtagagag
     6841 cagacctaag tcagtcagcc cctgaatgtt ggagtgacca cgcaatgcgt tcacgccgcc
     6901 accggccata cccatgttac cgagcagcaa ctggatcatc gccatagtac ggatgttctg
     6961 cgcacccacg gtgtgctgcg tccagcccag cgcgtacagg aaggtggttg tgcgatccgg
     7021 tgcgctggtg gaggccagca cttcacacac tttcaggaag tcggcttttg gcgtaccaca
     7081 gatgttttca acaacgtccg gcgtgtagcg ggaaacgtgc gctttcagca ggttccacac
     7141 acagcgcgga tgagtcagtg tttcatcgcg tttcgcatag ccgttttcat cgaactgata
     7201 gttccaggac gatttgtcgt actggcgttt ttcagcgtca taaccgctga acaggccatc
     7261 ttcgaaagca aaatcatcac gcaccagtag gctggcgttg gtgtaatgtt taacgtattc
     7321 ggcgttgatt ttgttgtttt cgatcaggta gcgcaaaatg ccagacagga acgtaatgtc
     7381 cgtaccggaa cgaataggtg cgtaaatatc cgccacagaa gcggtacgcg taaaacgggg
     7441 atcgacaacg atcaaggttg catcgttgtt gtttttcgct tccatcgccc agcggaaacc
     7501 gacgggatgc gcttcagcag cgttaccgcc cattaccatt acgacgttag cgtttttgat
     7561 atccacccag tggttggtca tagcaccgcg accaaatgtt ggagcaagac ttgctaccgt
     7621 tggtccgtgt cagacgcgcg cctggttgtc taccgccagc atcccgaggg agcgggcaaa
     7681 tttctgggtc agcatcccgg tttcgttgct ggcaccggag gcacacagca taccggtaga
     7741 aagccaacgg tttaccgtta cgccctgctc gttcttttca ataaagttag cgtcacggtc
     7801 agctttcatc agcttcgcaa tacgggagaa tgcttcttcc cagctaatgc gctgccattt
     7861 gtcagaacct ggcgcacgat atttcgggta gcgcagacgg ttttcactgt tgacgtaatc
     7921 cagcaaaccg gcccctttcg ggcacagcgc accacggctt accggatgat ccgggtcacc
     7981 ttcaatgtga taaatcgctt ctctggcgtt ttttgcgcca tcacccaggc tatacatcaa
     8041 tagcccgcaa cctacggaac agtatgtgca ggtgttacgg atctctttag cgcgtaataa
     8101 tttgtagttt cgcgcctgag ccagtgcttg cttcggggca aagcccaatg ccgctaccgt
     8161 tgttccagcc ataccgcccg cgcagatttt aaaaaattgt ctgcgactga cgtccattgc
     8221 tttcctcttt tttcaggggt attactgcgc gaggaaacta acagtaaaat tctgaatctt
     8281 tttgcgcgaa atcaaaaaga acacgtgttt gccacaaaat aatccgcctg cgcaaattga
     8341 cctacctcaa tagcggtaga aaaacgcacc actgcctgac aggccagtta aaaaaatgct
     8401 ataaaattca gcttaatttt taacggcaag agagacaaaa cagcgagcat gacacgacaa
     8461 aaagcaacgc tcatagggct aatagcgatc gtcctgtgga gcacgatggt aggattgatt
     8521 cgcggtgtca gcgaggggct cggcccggtc ggcggcgcag ctgctatcta ttcattaagc
     8581 gggctgctgt taatcttcac ggttggattt ccgcgtattc ggcagattcc gaaaggctat
     8641 ttactcgccg ggagtctgtt attcgtcagc tatgaaatct atctggcgct ttccttaggg
     8701 tatgcggcga cccgtcatca ggcgattgaa gtgggtatgg tgaactatct gtggcccagc
     8761 ctgacaattc tctttgccat tctgtttaat ggtcagaaaa ccaactggct gattgtacca
     8821 ggattattat tagccctggt cggcgtctgc tgggtgttag gcggtgagaa tgggttacat
     8881 tatgatgaaa tcatcaataa tatcaccacc agcccattga gttatttcct ggcgtttatt
     8941 ggtgcgttta tctgggcagc ctattgcaca gtaacgaata aatacgcacg cggatttaac
     9001 ggaattaccg tttttgtcct gctaacggga gcatgtctgt ggatttacta ttttctcacg
     9061 ccacaaccag aaatggtatt tagcacgccc gtcatgatta aactcatctc tgcggcattt
     9121 accttaggat ttgcttatac tgcatggaat gtcggtatat tgcatggcaa tgtcaccata
     9181 atggcggtag gctcgtattt tacgcctgta ctttcctcag cgcttgcagc cgtgctgctc
     9241 agcgccccgc tgtcgttctc gttctggcaa ggcgcactaa tggtctgcgg cggttccctg
     9301 ctctgctggc tggcgacacg tcgtggttaa aataattgcc gggtctacgg cccggcagtt
     9361 aatcagataa attccgacaa t
//
LOCUS       DAEBXN010000147         9328 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.147, whole genome shotgun sequence.
ACCESSION   DAEBXN010000147 DAEBXN010000000
VERSION     DAEBXN010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9328
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.147"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(139..1041)
                     /locus_tag="KJL99_003743"
                     /pseudo
     CDS             complement(139..1041)
                     /locus_tag="KJL99_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
     gene            1101..1379
                     /locus_tag="KJL99_003744"
     CDS             1101..1379
                     /locus_tag="KJL99_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438320.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNQVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(1380..1907)
                     /gene="gadE"
                     /locus_tag="KJL99_003745"
     CDS             complement(1380..1907)
                     /gene="gadE"
                     /locus_tag="KJL99_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HBD4438321.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(2706..3278)
                     /gene="hdeD"
                     /locus_tag="KJL99_003746"
     CDS             complement(2706..3278)
                     /gene="hdeD"
                     /locus_tag="KJL99_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HBD4438322.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            3533..3865
                     /gene="hdeA"
                     /locus_tag="KJL99_003747"
     CDS             3533..3865
                     /gene="hdeA"
                     /locus_tag="KJL99_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HBD4438323.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            3981..4307
                     /gene="hdeB"
                     /locus_tag="KJL99_003748"
     CDS             3981..4307
                     /gene="hdeB"
                     /locus_tag="KJL99_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HBD4438324.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            4371..5017
                     /locus_tag="KJL99_003749"
                     /pseudo
     CDS             4371..5017
                     /locus_tag="KJL99_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
     gene            complement(5059..5589)
                     /locus_tag="KJL99_003750"
     CDS             complement(5059..5589)
                     /locus_tag="KJL99_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBD4438325.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(5745..6311)
                     /gene="slp"
                     /locus_tag="KJL99_003751"
     CDS             complement(5745..6311)
                     /gene="slp"
                     /locus_tag="KJL99_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HBD4438326.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            complement(6558..7781)
                     /locus_tag="KJL99_003752"
     CDS             complement(6558..7781)
                     /locus_tag="KJL99_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438327.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNVKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQIHNIRYEKDKDSVCQIIVVVNPEPNNPIITKILVEEGIPERFSV
                     QTVSSDNKNFAGQRADLPTDIRDIQSLYLKMAKLYIEHSKNENRMEALAGCDFIDFNM
                     TGQDMSKLVLTLSKFYFEDLLNINFTDANLFNTIFSHKENPIPKLHKYEQHLDKQING
                     LFSTLLTINDNSLRAKAEIASRIIDFLEAKVVNLSFDDILKYKQEFKKICYKQLQEFT
                     TPSLYNKIQKWATMSKNEFIEFHYETLQPEKISYPFYLKRDLPNEKDINYGVEIEIPS
                     GKRIRLSNHYQNIIP"
     gene            complement(8362..>9328)
                     /locus_tag="KJL99_003753"
     CDS             complement(8362..>9328)
                     /locus_tag="KJL99_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBD4438328.1"
                     /translation="EMFVFLAVELSLLFIVISAGVSLIRQKVPDHKIQQMMGARKGRG
                     YLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLNPIIVGLMWMTFG
                     WKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYTPIKV
                     SCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFIY
                     GFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMALI
                     IGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
BASE COUNT         2719 a         1857 c         1939 g         2813 t
ORIGIN      
        1 tttatcgata tcgaagcatt gagcattttt aactcgtttt atcttgaatg caatcggcgc
       61 ataacttatt gaatcccatc aaatcccgtc tttttttacc cattcccggt tatcttgaat
      121 aaattaccta gtcataaata cattctggcg gctaccttca tccactaatg ccgtgacata
      181 catgccaggc agcaagtcac catttgggtt ggggaaaatc gcccgtaacg tcacggagcc
      241 cgtggtttca tcaaccgtcg ggtcggagaa tttcagcgtg ccggtctggc tgtaacgttt
      301 accattttcc agattgagct gtactggcgt actgccctga acctgtttga tttgcccact
      361 ggcgacctct tctttcatgc gtaagaaatc ttgcaccgac tgcgtgagat cgacataaat
      421 cgggtccaga cgttgtacgg taaccagcga atctgcctga ttagcggtaa cgagtgcgcc
      481 gacggtcacc gacgatttcc cgctgacgcc cgtaatcggc gaggtgacat tcgcgtattg
      541 cagattgatc gtcgcctgtt caacagccgc tttggcgacg gtgacattgg cttctgcttc
      601 attcagctgg gtgcgcgcgg tgtcgtaatc ctgacggcta acgtagttgg tcttcagcaa
      661 cgatgcctgg cggttaaagg tgatgcgggc attgctggcg gtagagagcg ctttcgccag
      721 cgagcctttg gcggagttta gctcggcctg taaaggtgca ggatcaatct gatacagcga
      781 atcgccctgg ttcactttat cgccttcgat aaagttgcgt ttaatgataa taccgcccac
      841 ctggggacgt atctcggcaa cttcataagg aacggttcta ccgggcaatt cgctcaacac
      901 attgaccgaa ccaggggaga gtgtgacgac accgacctca ggcgtcatgg cggcggcgtt
      961 ttccgccgat ttgtcatcgc aggcggtgag catcgcgccg cagaataaca acggtattaa
     1021 cagctttctt cttctgttca ttttagtccc tgaaaattct tgagaaataa cggttcttta
     1081 aattgcgcgc gaggagagat atgagggatt tacagaccag cggcatagtg gggttgtcag
     1141 ccagtaaggt gggatacagg cacagtgatc gacatggtga ggtcaacgac aaaaaaaatg
     1201 ttgccgttct gccaacagtt cctgccagca ttcgggcaac gaagggttct actggtggat
     1261 acacatacca ggggaatcaa gtcgttttag cctctccttt agtaatcacc gggggtaatg
     1321 aaatcagcat ctgcatccct cgtcatgcca gccatcaatt tcagttgctt atgtcctgac
     1381 taaaaataag atgtgatacc cagggtgacg atgtcgctca tacgtcttag tttcagcttg
     1441 tacataatgt tgaatttctg aatctttaat gtttgttgcg aaatattgag cgtcgacgtg
     1501 atattgcttt catttttatt tttcagaatc agttgcataa ttttgaactg attatgtgaa
     1561 aaacataatt gtgaattctt atggggcaag tgtttaccat aagtaatctc aagaagattt
     1621 ttgatacttt ctttgcggct aacaaaataa ataggcgctt tagcttttag tttactgatg
     1681 tgataaggtg ccaaaacaat aattctctcg gcatctaatt tctccagaaa tttaatcgct
     1741 tcttcatcaa ggatatgatt gtgataggta tcaatgataa ccttgaaggg tttattgcct
     1801 acttttttga tctctgacag ggaattgatt tttatcattt cgtgattatc tttcaactgc
     1861 caaaagccct gtaaaagaaa agaatctttc gtcatgagaa aaatcataac ttgctcctta
     1921 gccgttatcg tttcgaacat gtcatccttt tcattattta catccttgtc cgaatcgttg
     1981 ttcaatatag taaacgccta taagaacttt gcccttcgtg aactaatacg tctattaaca
     2041 ctaatcaata cttcagattt atccatcacg cactccatat attgatccta agtaagattc
     2101 ctggttgtta tcagcttgta aatagtaaac aaaaagttaa cgtttgcgga ttactgccca
     2161 agaataagtt atcaataacc agtttgtgat ctctgaagaa tattactaaa gttaaaatgt
     2221 aatccgattt aaatatcgag tctccttgtt tcgacttaag ctggcaattg gattgccagc
     2281 tttcttttaa cgcattgaaa ttacctgagt aaataacgca ttcatgtctt caggttttta
     2341 tatagcgtct ggagacacgg atatttatgc aatgtaatca acgccagttg caaaaaaatg
     2401 aacccggatt acttatttaa ccgcccatag catcaacgct tacggaactc tttgttcact
     2461 ttcccacagc acttttaaaa cattaaacct gactacggaa aatatcagcc atgataatgt
     2521 ttgaaatggc taatttgcca tggagtgaaa aaaattagat gaaattcagt aggttgaaat
     2581 aatcactagc aggtaattat ttcaatgata gtgcgcaatt gatctacaac actgcgtagc
     2641 ggagagagta ttaatcggat catagtcaca tcaagtgact atgatccggg tgacaaccgg
     2701 ggtaattatt gctgcttaac gaacaaactg gcgaagctga acaggctggc ggcgctaaat
     2761 atcagttcaa ttcccaccag tgtggaaacc agcgttacag acaccatcgg cgttgcacca
     2821 aggaatatcc aggcaatgac gatatccagc acaccaataa cgagctgtag ccagctgcct
     2881 ttcattgaac gctgacgata ccaactcatc aggcgaataa cccctgcaac acagaacaaa
     2941 ccggcaataa atgccgcaat ggcaaaaatg cccagctccg gtgcacggat gaagaaatag
     3001 ccgatcaata aataggcgac tgcgacgagg aaaccggata ataccggcca gaaattatga
     3061 ctgcggttgc tgaataaccc gacaataagc gcaatacccg agcagattaa taatgcaccc
     3121 actactgtgc ttaaaatatc gccagagacg aacgggaaac tgatacacag caacccgacg
     3181 ataaacagca gcacggcaat aaactggatt gctctgcgat gctttttcag catctccaga
     3241 tcaaacttca aaattgttgc cttatctata tataacatag aaccacccta taaaattaag
     3301 aagaaaatcc cctgctatca atctatgcca aaaacgcgtc taagaatgca gtcgatttaa
     3361 taaaaatttc ctaattgcag tatctgatgc atctgtaact cattgtattg aaataaaaat
     3421 atctgatttt gatattttcc atcaacatga catatacaga aaaccaggtt ataacctcag
     3481 tgtcgaaatt gattcgtgac ggctctttca ctttatagtt gaggatatta cgatgaaaaa
     3541 agtattaggc gttattcttg gtggtctgct tcttctgcca gttgtgagca atgcagcgga
     3601 tgcgcaaaaa gcagctgata acaaaaaacc ggtcaactca tggacctgtg aagatttcct
     3661 ggctgtggac gaatccttcc agccaactgc agttggtttt gctgaagcgc tgaacaacaa
     3721 agataaacca gaagatgcgg ttttagatgt tcagggtatt gcaaccgtaa ccccagctat
     3781 cgttcaggct tgtactcagg ataaacaagc caactttaaa gataaagtta aaggcgaatg
     3841 ggacaaaatt aagaaagata tgtaattccg ggaatgcgtt acatcgtact tccttgcata
     3901 ttgaacaggc cggaatatct tctttaaaag cagctattcc tcctgttcat atataatctc
     3961 tatattgaat gggttacaaa atgaatattt catctctccg taaagcgttt atttttatgg
     4021 gcgctgtagc ggctttgtca ctggtgaacg cacaatctgc gttggcagcc aatgaatctg
     4081 ctaaagatat gacctgccag gaatttattg atctgaatcc aaaagcaatg accccggttg
     4141 catggtggat gctgcatgaa gaaacagtat ataaaggtgg cgataccgtt actttaaatg
     4201 aaaccgatct cactcaaatt cctaaagtga tcgaatactg taagaaaaac ccgcagaaaa
     4261 atttgtatac cttcaaaaat caagcatcta atgacttgcc gaattaatga ggtacaagta
     4321 aaaaggagta gcaagttgag ccatcttgct gctccttttt gcatttttat atgacagcag
     4381 aatttattat acgtcttata ctcgcggcaa ttgcctgtgg cgctattggc atggaaaggc
     4441 aaatgcgcgg caaaggagca gggttacgca cacatgtatt aattggcatg ggaagcgccc
     4501 tgtttatgat tgtttcgaaa tatggttttg ctgacgtgct gtctttagat cacgtcggac
     4561 tcgaccccag ccgtattgct gctcaggtgg tgacgggcgt cgggtttatc ggtgcaggta
     4621 acattctcgt tcgtaaccaa aatattgtag gtctgacgac ggcagcggat atctgggtga
     4681 ccgccgcata ggtatggtta ttggcagcgg tatgtacgaa ctgggtattt atggttcagt
     4741 gatgaccttg ttggtgctgg aagtcttcca tcaattaacc ttccgcctga tgaacaaaaa
     4801 ttaccattta cagctgacat tagtgaacgg taatacggta tcaatgctcg actggtttaa
     4861 gcagcaaaaa ataaaaactg atttagtttc gttgcaggaa aatgaagatc atgaagtggt
     4921 cgccattgat atccagcttc atgccacaac gttgatagaa gacctgttgc gattattaaa
     4981 aggtatggca ggggtgaaag gcgtttcaat tagctaatct gtgtggcagc gtagccagac
     5041 tcaccgtaag cctgaaattc acaccagata atcaatatgc tgatggcgta ccagctcatt
     5101 gatccgctta agattgagga tcatcatgat attgtgttta tggcagtaga acgtttttag
     5161 cgatattttc agtttctcca gaatatcttc agttgtcatt tgattcgcaa tgcagctcag
     5221 aacctgatgt tgcttgcgcg aaagtgacac attcgcttca ggcacagagc agtaactgcc
     5281 attgatgagt gcgacaaaat caatgatagt gatagtccgc ggaacaaagg tcaccggtac
     5341 tttccccaga cagcgtttaa tattaaaggg ggagaaaaca atgacatgga caggttttaa
     5401 tcgttcaatt tgagtgagaa aattagaatg aaatacatta ttcattaatg tatcaataat
     5461 gacgaaggca ttctgatgca acattacgtc gatctcttct tcgttctgta tatgaacgac
     5521 attaccttta ctcagaatgt ttttcatcgc ggtgaagaac atcgtatccc tggtaattat
     5581 aagaaacata accgactcct ggtgcggaca ttaactttat aatcagtttg ccaaaggtaa
     5641 ttaacaggac agggcttata ataataatag ttagcttact gtatattaat tttatgtaat
     5701 acatgatgca gatgctgaga aagcatctgc atctttcggt ggtgttattt gaccagctca
     5761 ggtgttacct gactcaccgc attggtgtag taaggcgcac cccagcccgg ttccggccag
     5821 aatgcaccat agccgtaatc ccacaggtta taggtggtat taaccacttc tctcaaatgc
     5881 cacacctgga tgccctgcat attcacttcc aggaagttat acgggacttt attgataaag
     5941 cctggttgtt caccctgaat ggtaccgagg atggtaacaa agtgattacg atagttcact
     6001 ggatcaagga agccgctttg tctggcgagc agtcggccct gatagttggc ttcaatatca
     6061 ggcttcgcat agctatccaa cggtaataca gcgatttcta acaacgtatc cgttttgcca
     6121 ttgataacgt tgataacctt cccaccaaag cgcgcttgtt gaccaacata taaccccggc
     6181 tggttatgaa cagcaacaaa acttttttga atatcaggtt ggttattgcc tttgatattt
     6241 tgcggaattg aactacatgc ggcaagcaaa aatgaaaggc tgaggatgag tgcacctttt
     6301 gtcatgttca tgttactatc cttatcaaca aatattaaaa ccataataga tatttcttta
     6361 tagttttcat ctgattctga ggtgatatgc ctgatagtaa cgtaaacctg tctgatgttt
     6421 atatcccttc tgtttagttc aattccgtta ttttggggcg tggtatgagt atccatcgcc
     6481 caataatata accctttgtt ttaaaattac ataaaatcta tttttattca atgattataa
     6541 agtgctaata atcaatatta tggtattata ttctgataat gatttgataa gcgaattctt
     6601 ttgccagatg gtatttctat ttccacccca taatttatat ctttttcatt aggcagatct
     6661 ctctttaaat aaaaggggta tgaaatcttt tcgggttgaa gtgtctcgta atgaaattct
     6721 atgaattcat ttttagacat tgttgcccat ttttgaattt tattgtatag agagggtgtt
     6781 gtaaactctt gaagttgttt ataacatatt tttttgaact cttgcttata ttttaatatg
     6841 tcatcaaatg aaagattcac tacctttgcc tctaagaaat caattattct cgaagcaatt
     6901 tccgctttcg cgcgtaaaga gttgtcatta atagttaaca atgtagaaaa caaaccattg
     6961 atttgtttgt caagatgttg ttcatattta tgtagtttgg ggatgggatt ttccttgtgt
     7021 gaaaaaatag tgttaaataa atttgcgtca gtgaaattta tattaagtag atcttcaaaa
     7081 taaaattttg ataaggttag gactagcttg gacatatctt gccctgtcat gttgaaatca
     7141 ataaagtcgc atccagcaag ggcttccatc ctattttcat ttttactgtg ctcaatatac
     7201 agcttcgcca tttttaagta caagctttga atgtctctta tatcagttgg taagtcagct
     7261 cgttgcccag caaaattttt attatcggaa gaaacggttt ggacactgaa tctttcgggt
     7321 attccttctt ctacgagaat tttagttatg attggattgt tgggttctgg attaacgaca
     7381 acaatgattt gacatacaga atccttatcc ttttcgtatc taatgttatg aatctgatta
     7441 agtattgatt gtgtctgttg cgtaagtggt gtcgaactta tcgtttcaat gttattaata
     7501 agacttacaa gttgaaccac aacttttgag tagtctgcat ttgtatatgt ggtgttcgtg
     7561 tggcatgcat taatttgttg gagttgttgt tgcgctaaat caaattgttc agggttgtat
     7621 ccttctccag agaatgtgga attaatgggt tgttgttctt cccaattatc acaaggccac
     7681 ttcggtgtgt ctggcgtttt aacattatcg gtatttgttg tgcagttata aatatcgccg
     7741 tgatatgtat ttattatacc tggggtaaag tcaattgaca taacattcct tattcatcat
     7801 ctgttttatg ctaatgtttt taaattggat aattagaaca ggaatggatt ttcttctaca
     7861 cacaataaat ataatgcgtt atatcttcct gatataatta cctttaaatt ccctttcatt
     7921 tccacccggt aatatttgtg tataaaaaaa gggcagtgat agtatcgtaa aggaaaacgt
     7981 taacaactat caaaaaacgc tcattttaaa aagttattat gttctccgat agtgtgtgaa
     8041 gtaatatttt tgtgatgttt tagatgtgta ataatgaacc aggttaaagt gtatttaaaa
     8101 atgtgttgta ttagcgactg aaatctgcct tttaaaaagg aggagggtac tgagacaaag
     8161 agtcattgct tctccgataa aatgtcagag aagcatgatt acgtggttat tgtctgttga
     8221 tgacttgttg accgagccgt catcattctc aatgcattag ttaaatcatt tgtgattttt
     8281 aaggattatc ctgtctatga ttttctactt agaaggatat ggcactaact atgcatctta
     8341 tagtttactg ctggcatcta tttaaaatag caattgagtc aaatagccca tcaagatagc
     8401 catacctaat ataaccgtca ggaatgcagc tatcatcggc attctgaaca ttgatttaag
     8461 taatatcact tccgtcaggc ttgcgccggc actgccgatg attaatgcca ttaatgctcc
     8521 cagacccatt ccttttgtca tcagaacaga tgccagaggg ataaccgcct cagcccggat
     8581 atacagcgga ataccgacaa cggcgcttaa tgggatggcg aagggattat ctgctcctgc
     8641 atgagcggca atccacgctg agggaataaa gccataaata aaagatccta ttaatactcc
     8701 taacaaaaga tatggcagta catctttaaa ctgttgcaac gcatctttgg tggctagttt
     8761 tatgggatta atacttacta cagctttggt actgcaacag gtaactacgg ggttctcaat
     8821 ggctttagcc gctggactac aacaactcac ttttatgggc gtatatgtcg ttcccggcga
     8881 agtttttgct ggagcacaac aatttgctga tgagctttta ctggcaatga tatgacgttc
     8941 aaaccccaga gaatccagga taatactggc aagaacggag acgccggctg cgataatcgc
     9001 gtacaacaag gtgactttcc aaccaaaggt catccacatt agtccgacga taatgggatt
     9061 aagtaatggt gaaacaaata aaaaagtgag agtcggacca aacccggctt tcgctgacaa
     9121 caatccacgt aacatgggta ttgtcgagca actacagaac ggggtaacgg ctcccaacag
     9181 agcagccagg agatagcctc tcccttttct ggcccccatc atctgctgga ttttatggtc
     9241 tggcaccttt tgtcttatca ggctgacacc ggcactaatc actataaaca gcaacgaaag
     9301 ctcgacggcg agaaacacaa acatctct
//
LOCUS       DAEBXN010000148         9312 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.148, whole genome shotgun sequence.
ACCESSION   DAEBXN010000148 DAEBXN010000000
VERSION     DAEBXN010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9312
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.148"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..309
                     /locus_tag="KJL99_003754"
     CDS             <1..309
                     /locus_tag="KJL99_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HBD4438329.1"
                     /translation="RFINTDKAPAYGRALALLKREGRCPSDVEHRQIKYRNNVIECDH
                     GKLKRIINATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPLGEMRLVSRVFEM
                     "
     gene            361..723
                     /locus_tag="KJL99_003755"
                     /pseudo
     CDS             361..723
                     /locus_tag="KJL99_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389366.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
     gene            854..1642
                     /locus_tag="KJL99_003756"
     CDS             854..1642
                     /locus_tag="KJL99_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA5"
                     /protein_id="HBD4438330.1"
                     /translation="MGEFFPAQVFKQLSHARAVIERHLAATLDTIHLFGSAIDGGLKP
                     DSDIDLLVTVSAAPNDSLRQALMLDLLKVSSPPGDGGTWRPLELTVVARSEVVPWRYP
                     ARRELQFGEWLRHDILSGTFEPAVLDHDLAILLTKARQHSLALLGPSAATFFEPVPKE
                     HFSKALFDTIAQWNAESDWKGDERNVVLALARIWYSASTGLIAPKDVAAAWVSERLPA
                     EHRPLICKARAAYLGSEDDDLAMRVEETAAFVRYAKATIERILR"
     gene            1848..2195
                     /locus_tag="KJL99_003757"
     CDS             1848..2195
                     /locus_tag="KJL99_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="HBD4438331.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     gene            2189..3028
                     /gene="sul1"
                     /locus_tag="KJL99_003758"
     CDS             2189..3028
                     /gene="sul1"
                     /locus_tag="KJL99_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /protein_id="HBD4438332.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     gene            3156..3359
                     /locus_tag="KJL99_003759"
     CDS             3156..3359
                     /locus_tag="KJL99_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438333.1"
                     /translation="MDSEEPPNVRVACSGDIDEVVRLMHDAAAWMSAKGTPAWDVARI
                     DRTFAETFVLRSELLGIASENGK"
     gene            3515..4720
                     /gene="chrA"
                     /locus_tag="KJL99_003760"
     CDS             3515..4720
                     /gene="chrA"
                     /locus_tag="KJL99_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004896675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HBD4438334.1"
                     /translation="MTNNPTDDSRPWSVFLIFLRLGLTSFGGPIAHLGYFRAEFVTRR
                     RWLSERSYADLVALCQFLPGPASSQVGIAVGLSRAGYSGALAAWAGFTLPSAIALILF
                     ALGISSYGDYVSQGALHGLKVVAVAVVAQAVWGMARNLCTDGLRVTIMAIATCVVLLV
                     PSAWGQVGVIAIAGIAGRLLFKPAKVVEHDPLPITVSHRAGVLWLSLFFVLLIGLPVL
                     AELMPSQTMAMVDSFYRVGSLVFGGGHVVLPLLQAEVVPSGWVNNESFLAGYGAAQAV
                     PGPLFTFAAFLGASMNTAPSGWIGGIVCLLAIFAPSFLLVVGSMPFWERLRRNTGIQA
                     ALAGINAAVVGLLLAALYQPVWTSAIFQPQDFGLALVALVALMFWKLPPWLVVLGSGA
                     AGWLLSVAL"
     gene            4731..5036
                     /locus_tag="KJL99_003761"
     CDS             4731..5036
                     /locus_tag="KJL99_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBD4438335.1"
                     /translation="MTDKDLYGGLIRLHILHHAAEEPVFGLGIIEELRRHGYEMSAGT
                     VYPMLHGLEKKGYLTSRHERTGRRERRVYDITEQGRTALADAKTKVKELFGELVEGG"
     gene            5080..5145
                     /locus_tag="KJL99_003762"
                     /pseudo
     CDS             5080..5145
                     /locus_tag="KJL99_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229646.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            5263..6027
                     /locus_tag="KJL99_003763"
     CDS             5263..6027
                     /locus_tag="KJL99_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS6100 family transposase"
                     /protein_id="HBD4438336.1"
                     /translation="MTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEMLAERGISVDH
                     TTIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYRAVDKRGDTID
                     FYLSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELKREGKLDRETA
                     HRQVKYLNNVIEADHGKLKILIKPVRGFKSIPTAYATIKGFEVMRALRKGQARPWCLQ
                     PGIRGEVRLVERAFGIGPSALTEAMGMLNHHFAAAA"
     gene            complement(6520..7104)
                     /locus_tag="KJL99_003764"
     CDS             complement(6520..7104)
                     /locus_tag="KJL99_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBD4438337.1"
                     /translation="MPRPKLKSDDEVLEAATVVLKRCGPIEFTLSGVAKEVGLSRAAL
                     IQRFTNRDTLLVRMMERGVEQVRHYLNAIPIGAGPQGLWEFLQVLVRSMNTRNDFSVN
                     YLISWYELQVPELRTLAIQRNRAVVEGIRKRLPPGAPAAAELLLHSVIAGATMQWAVD
                     PDGELADHVLAQIAAILCLMFPEHDDFQLLQAHA"
     gene            complement(7104..8342)
                     /locus_tag="KJL99_003765"
     CDS             complement(7104..8342)
                     /locus_tag="KJL99_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD4438338.1"
                     /translation="MSERRYSPLATLFAATFLFRIGNAVAALALPWFVLSHTKSAAWA
                     GATAASSVIATIIGAWVGGGLVDRFGRAPVALISGVVGGVAMASIPLLDAVGALSNTG
                     LIACVVLGAAFDAPGMAAQDSELPKLGHVAGLSVERVSSLKAVIGNVAILGGPALGGA
                     AIGLLGAAPTLGLTAFCSVLAGLLGAWVLPARAARTMTTTATLSMRAGVAFLWSEPLL
                     RPLFGIVMIFVGIVGANGSVIMPALFVDAGRQVAELGLFSSMMGAGGLLGIAIHASVG
                     ARISAQNWLAVAFCGSAVGSLLLSQLPGVPVLMLLGALVGLLTGSVSPILNAAIYNRT
                     PPELLGRVLGTVSAVMLSASPMVMLAAGAFVDLAGPLPGLVVSAVFAGLVALLSLRLQ
                     FATMAAAATASAPTHTEGEH"
     gene            complement(8339..9244)
                     /locus_tag="KJL99_003766"
     CDS             complement(8339..9244)
                     /locus_tag="KJL99_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(A) family macrolide 2'-phosphotransferase"
                     /protein_id="HBD4438339.1"
                     /translation="MTVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVD
                     DGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA
                     MVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVA
                     DDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMI
                     DWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVT
                     YALFALDSGNEEYLAAAKAQLAAAE"
BASE COUNT         1934 a         2676 c         2746 g         1956 t
ORIGIN      
        1 cgattcatca acacggataa agcgcccgcc tatggtcgcg cgcttgctct gctcaaacgc
       61 gaaggccggt gcccgtctga cgttgaacac cgacagatta agtaccggaa caacgtgatt
      121 gaatgcgatc atggcaaact gaaacggata atcaacgcca cgctgggatt taaatccatg
      181 aagacggctt acgccaccat caaaggtatt gaggtgatgc gtgcactacg caaaggccag
      241 gcctcagcat tttattatgg tgatcccctg ggcgaaatgc gcctggtaag cagagttttt
      301 gaaatgtaag gcctttgaat aagacaaaag gctgcctcat cgctaacttt gcaacagtgc
      361 catcaatggc tccttgtcgg aagaaaaaca tttgactcta tgggtgttct tccaaatcgc
      421 aaatatgcag tagtgtcaaa gaacggaatt tcaagctcaa atgaaaacgt cctagttttt
      481 ccttcaatag aaaatgcttt gaaagagcta tcaaaagtta cagatcatgt atatgtctct
      541 ggcgggggtc aaatctataa tagccttatt gaaaaagcag atataattca tttgtctact
      601 gttcacgttg aagtcgaagg tgatatcaaa ttccctataa tgcctgagaa tttcaatttg
      661 gtttttgaac agttttttat gtctaatata aattatacat accagatttg gaaaaaaggc
      721 taacaatgcg ttgcagcacc agtcgcttcg ctccttggac agcttttaag tcgcgtcttt
      781 gtggttttgc tgcgcaaaag tattccacaa agccgcaact taaaagctgc cgctgaactt
      841 aacgttaggc atcatgggtg aatttttccc tgcacaagtt ttcaagcagc tgtcccacgc
      901 tcgcgcggtg atcgagcgcc atctggctgc gacactggac acaatccacc tgttcggatc
      961 tgcgatcgat ggagggctga agccggacag cgacatagac ttgctcgtga ccgtcagcgc
     1021 cgcacctaac gattcgctcc ggcaggcgct aatgctcgat ttgctgaaag tctcatcacc
     1081 gccaggcgat ggcggaacat ggcgaccgct ggagctaact gttgtcgctc gaagcgaagt
     1141 agtgccttgg cgctatccgg cgcggcgtga gcttcagttc ggtgagtggc tccgccacga
     1201 catcctttcc ggaacgttcg agcctgccgt tctggatcac gatcttgcga ttttgctgac
     1261 caaggcgagg caacacagcc ttgcgcttct aggcccatcc gcagccacgt ttttcgagcc
     1321 ggtgccgaag gagcatttct ccaaggcgct tttcgacact attgcccagt ggaatgcaga
     1381 gtcggattgg aagggtgacg agcggaacgt cgttcttgct cttgctcgca tttggtacag
     1441 cgcttcaact ggtctcattg ctcctaagga cgttgctgcc gcatgggtat cggagcgttt
     1501 gcctgccgag catcggcccc tcatctgcaa ggcacgcgcg gcgtacctgg gtagcgagga
     1561 cgacgaccta gcaatgcgcg tcgaagagac ggccgcgttc gttcgatatg ccaaagcaac
     1621 gattgagaga atcttgcgtt gagcggcatg tgcgaaaagt gcatcgaccc gcgccgaggg
     1681 catctgatgc ctaactcggc gttcaagcgg acgggctgcg cccgccgctc aactatgcgt
     1741 tagatgcact aagcacataa ttgctcacag ccaaactatc aggtcaagtc tgcttttatt
     1801 atttttaagc gtgcataata agccctacac aaattgggag atatatcatg aaaggctggc
     1861 tttttcttgt tatcgcaata gttggcgaag taatcgcaac atccgcatta aaatctagcg
     1921 agggctttac taagcttgcc ccttccgccg ttgtcataat cggttatggc atcgcatttt
     1981 attttctttc tctggttctg aaatccatcc ctgtcggtgt tgcttatgca gtctggtcgg
     2041 gactcggcgt cgtcataatt acagccattg cctggttgct tcatgggcaa aagcttgatg
     2101 cgtggggctt tgtaggtatg gggctcataa ttgctgcctt tttgctcgcc cgatccccat
     2161 cgtggaagtc gctgcggagg ccgacgccat ggtgacggtg ttcggcattc tgaatctcac
     2221 cgaggactcc ttcttcgatg agagccggcg gctagacccc gccggcgctg tcaccgcggc
     2281 gatcgaaatg ctgcgagtcg gatcagacgt cgtggatgtc ggaccggccg ccagccatcc
     2341 ggacgcgagg cctgtatcgc cggccgatga gatcagacgt attgcgccgc tcttagacgc
     2401 cctgtccgat cagatgcacc gtgtttcaat cgacagcttc caaccggaaa cccagcgcta
     2461 tgcgctcaag cgcggcgtgg gctacctgaa cgatatccaa ggatttcctg accctgcgct
     2521 ctatcccgat attgctgagg cggactgcag gctggtggtt atgcactcag cgcagcggga
     2581 tggcatcgcc acccgcaccg gtcaccttcg acccgaagac gcgctcgacg agattgtgcg
     2641 gttcttcgag gcgcgggttt ccgccttgcg acggagcggg gtcgctgccg accggctcat
     2701 cctcgatccg gggatgggat ttttcttgag ccccgcaccg gaaacatcgc tgcacgtgct
     2761 gtcgaacctt caaaagctga agtcggcgtt ggggcttccg ctattggtct cggtgtcgcg
     2821 gaaatccttc ttgggcgcca ccgttggcct tcctgtaaag gatctgggtc cagcgagcct
     2881 tgcggcggaa cttcacgcga tcggcaatgg cgctgactac gtccgcaccc acgcgcctgg
     2941 agatctgcga agcgcaatca ccttctcgga aaccctcgcg aaatttcgca gtcgcgacgc
     3001 cagagaccga gggttagatc atgcctagca ttcaccttcc ggccgcccgc tagcggaccc
     3061 tggtcaggtt ccgcgaaggt gggcgcagac atgctgggct cgtcaggatc aaactgcact
     3121 atgaggcggc ggttcatacc gcgccagggg agcgaatgga cagcgaggag cctccgaacg
     3181 ttcgggtcgc ctgctcgggt gatatcgacg aggttgtgcg gctgatgcac gacgctgcgg
     3241 cgtggatgtc cgccaaggga acgcccgcct gggacgtcgc gcggatcgac cggacattcg
     3301 cggagacctt cgtcctgaga tccgagctcc tagggatcgc ctcagaaaac ggaaaataaa
     3361 gcacgctaag ccgtaagtaa gcgtgctcct gtgaaagcca cagctaaaac tgcgtagtac
     3421 acatagaggt atttttttct agatttatat cgaatttcgt tatcgaattt cgcttattat
     3481 tgatcaactc gttgcagatc atgaaggcga aagtatgacg aataacccta ccgatgacag
     3541 cagaccatgg tcggtctttc ttatttttct gcggcttgga ttgacatctt ttggcggccc
     3601 cattgcgcac ttgggctact tccgcgccga atttgtcaca cggcggcgct ggctctccga
     3661 acggagctat gctgacttgg tcgcgctttg tcagttcttg ccagggcctg caagcagcca
     3721 ggtcggcata gcggtaggac tgtctcgggc tggatacagc ggggcgctgg ctgcttgggc
     3781 tggcttcacg ctgccgtctg ccatagcctt gatccttttt gcgctcggca tctccagcta
     3841 tggcgattac gtctcgcagg gcgcgttgca tggcttaaaa gtggtggctg tggccgtggt
     3901 cgctcaagca gtatggggca tggcgcgtaa cctatgcacg gatgggctgc gagtcaccat
     3961 catggcaatt gctacctgcg tcgttttact tgtgccgtcc gcgtggggac aggttggcgt
     4021 gattgctatc gcaggcatcg caggccggtt attgttcaag ccagcgaaag ttgttgagca
     4081 tgacccccta cctatcacgg tcagtcaccg ggccggcgtg ctttggctct cgctgttctt
     4141 tgtcttgctg attggcctgc cggtgttggc cgaactgatg ccaagtcaaa ccatggcaat
     4201 ggtggattcc ttctatcgtg tcggatcact ggtgttcggc ggtggtcacg ttgtgctgcc
     4261 attactgcaa gccgaagtgg tgccctccgg ctgggtcaac aatgaatcct ttctcgcggg
     4321 gtacggggca gctcaagcgg tgcccggccc tttgttcacg ttcgccgcgt ttcttggtgc
     4381 ctcgatgaac accgccccgt cgggctggat cggcggcatt gtgtgtctgc tggctatctt
     4441 cgcgccctcg ttcttgctgg tcgtcggatc aatgccattt tgggagcgtt tgcgccgcaa
     4501 tacaggcatc caagctgcgc tggccgggat caatgccgct gtagtcggct tgctgctggc
     4561 cgcgctgtat cagcctgtat ggactagcgc catctttcag ccgcaagact tcggcttggc
     4621 attagttgcc cttgtcgcac ttatgttctg gaagctcccg ccgtggctgg ttgtcctcgg
     4681 tagcggtgca gctgggtggc tgttgagcgt cgctctgtga ggcacaaaaa atgactgaca
     4741 aagacctcta cggcggtttg atccgcctgc acatccttca ccatgcagcc gaggaacctg
     4801 tctttgggct ggggatcatc gaagagctac gccgacacgg ctacgagatg agcgctggca
     4861 ccgtgtaccc gatgctgcac ggcctggaaa aaaaaggcta tctgacctca cgccacgaac
     4921 gcaccgggcg acgtgaacgg cgtgtttatg acatcactga acaaggcaga actgcacttg
     4981 ctgatgcgaa aacaaaggtc aaggagctgt tcggagagtt ggtagaaggt ggttgagttt
     5041 ttgcttctat gtcaggttga gctataccct aactggattg tcattttcag aagacgactg
     5101 caccagttga ttgggcgtaa tggctgttgt gcagccagct cctgacagtt caatatcaga
     5161 agtgatctgc accaatctcg actatgctca atactcgtgt gggctctgtt gcaaaaatcg
     5221 tgaagcttga gcatgcttgg cggagattgg acggacggaa cgatgacgga tttcaagtgg
     5281 cgccatttcc agggtgatgt gatcctgtgg gcggtgcgct ggtattgtcg ctatccgatc
     5341 agctatcgcg accttgagga aatgctggcg gaacgcggca tttcggtcga ccatacgacg
     5401 atctatcgct gggtccagtg ctacgccccg gagatggaga agcggctgcg ctggttctgg
     5461 cggcgtggct ttgatccgag ctggcgcctg gatgaaacct acgtcaaggt gcggggcaag
     5521 tggacctacc tgtaccgggc agtcgacaag cggggcgaca cgatcgattt ctacctgtcg
     5581 ccgacccgca gcgccaaggc agcgaagcgg ttcctgggca aggccctgcg aggcctgaag
     5641 cactgggaaa agcctgccac gctcaatacc gacaaagcgc cgagctatgg tgcagcgatc
     5701 accgaattga agcgcgaagg aaagctggac cgggagacgg cccaccggca ggtgaagtat
     5761 ctcaataacg tgatcgaggc cgatcacgga aagctcaaga tactgatcaa gccggtgcgc
     5821 ggtttcaaat cgatccccac ggcctatgcc acgatcaagg gattcgaagt catgcgagcc
     5881 ctgcgcaaag gacaggctcg cccctggtgc ctgcagcccg gcatcagggg cgaggtgcgc
     5941 cttgtggaga gagcttttgg cattgggccc tcggcgctga cggaggccat gggcatgctc
     6001 aaccaccatt tcgcagcagc cgcctgatcg gcgcagagcg acagcctacc tctgactgcc
     6061 gccaatcttt gcaacagagc ccgccgtgct agtctgctcg gtgatggtgg agtgaagcca
     6121 acccgcaatc gggttatgaa tctgcatcgc gattcgcaat cagctgtctc ttgagcatgt
     6181 cgaactcctg cgatgttatc tcgccgtgct cgtgctgcta caccaacttg tccaactcat
     6241 ccgccacacc gctccccacc acctgcgatg gccaggttgt ttgctgggcg cattggcgga
     6301 gtatcgtctc gacccgggac acccacaccg gcacaacctt acgggagtga ttcactgtca
     6361 aagaatcggc ccggtgctct gacgcaagta tcgggatggt caccatttgt aagccgtaga
     6421 cctgagtggt gatcaagact tcgataccac cgaccgtacc ggtactaatc gacgacggtc
     6481 gtgttcgtcg cctgccgcag ggactctgca cacctccgtt tacgcatgtg cctggaggag
     6541 ttggaaatcg tcgtgttcgg gaaacattaa acacaggatg gcagcgatct gagccagcac
     6601 atgatcagct agctcaccat ccggatcgac ggcccactgc atcgtcgcgc cagcgatgac
     6661 cgagtgcagg agcaactcag ctgccgcagg agcacctggg ggcagtcgct tgcggatccc
     6721 ctccaccacc gcgcggttcc gctggatcgc aagcgtgcgt agctccggca cctggagctc
     6781 gtaccaggag atgagatagt tcaccgagaa gtcgttgcga gtgttcatgc tccgaacgag
     6841 cacctgcaaa aattcccaga gcccttgcgg ccctgcgcct atcggtatcg cattcaggta
     6901 atgccgcacc tgctcgacgc cgcgctccat catcctcacc agcagcgtat cgcggttggt
     6961 gaagcgctgg attaacgctg cgcgggagag ccccacctcc tttgctactc cgctgagcgt
     7021 gaactctatg ggaccgcaac gcttcagcac tacggtggcg gcctcgagta cctcgtcatc
     7081 ggacttgagc ttggggcggg gcatcagtgt tcaccttctg tatgggttgg ggcggaggct
     7141 gtggctgccg ccgccattgt agcaaattga agacggagcg agagtagagc cacgagcccc
     7201 gcaaacacgg ccgatacaac gaggccaggg agcggaccag caaggtcgac aaacgcgccg
     7261 gccgcaagca taaccatggg cgaggctgac agcatcaccg ccgagaccgt gccgagtacc
     7321 cggccgagaa gttctggcgg cgtgcggttg tagatggcag cgttgagaat gggagagact
     7381 gagccggtca gcagtcccac gagcgcgccc aacaacatca gcaccggcac gcctggcaac
     7441 tgtgaaagca gaagcgagcc caccgcagag ccacaaaatg ccaccgccag ccagttctgc
     7501 gctgatatcc gggcgccgac cgacgcatga atggcaatgc caaggagacc accagccccc
     7561 atcattgagg agaacagccc gagctctgct acttggcgtc ctgcatctac aaacagcgca
     7621 ggcatgatga cgctgccgtt ggcgccaacg atgcccacga agatcatcac tataccaaag
     7681 agagggcgca gcaggggttc gctccagaga aaagcgacgc cggcgcgcat ggagagagtc
     7741 gccgtcgtgg tcatcgtccg agcggcacgc gcgggaagca cccacgcgcc gagcagacct
     7801 gcaaggacgg agcagaacgc cgtcagcccg agcgttggcg cagcgccaag caggccgatt
     7861 gcggcccccc caagggccgg gccacctaga atcgcgacgt tcccgatcac cgctttcagt
     7921 gacgagacgc gctcaacgga gagcccggcg acgtggccga gtttgggcag ctcactgtcc
     7981 tgcgcggcca taccgggtgc gtcgaacgcg gcaccgagca ccacgcaagc gatcagccca
     8041 gtgttcgaga gggcgccaac ggcatcgagc agtgggatgc tcgccatggc cacgccgccc
     8101 accacacccg agatcaatgc gacgggcgcg cgcccgaacc gatcgacgag gccaccacca
     8161 acccacgcgc cgatgatggt cgcgatgacg ctgctagcgg ccgtggcgcc cgcccaggcc
     8221 gcgctctttg tatgagacag gacgaaccat ggaagcgcga gggccgccac cgcgttgccg
     8281 atccggaaga gaaaggtcgc cgcgaacagc gtcgcgagcg ggctatatcg acgttcgctc
     8341 attccgctgc ggcgagctgc gccttcgccg cagcgaggta ctcttcgtta cccgagtcga
     8401 gggcgaagag tgcgtaggtg accgccccga acgcaaggcg ctccgcgatg tggtgggcga
     8461 gccgcggcca cacccggcca ccggccgctt catacgtgag gaggagcttc gcgagcccct
     8521 cttcaccaaa gaccataagg tgcgcggcca tgtcgatggc agggtcatca acgcgggcct
     8581 cgctccagtc gatcatcccg ctgacgcgct ccgtgttgtc gatgagcaca tggcccacgt
     8641 agagatcgcc atgcaccacc acggagaaat ctggccacga cgaatcgtcg tcgagccagc
     8701 gctgccaccg gtggaggcgc ttgtcgttca ccacgaactc gcgtcggacg cggtcaacgt
     8761 cgtcggccac cttctgacgg gcctgcgtcg gtgtacggat gagcatcccc gcatccacgg
     8821 cggcggaaat ggggacggca tgcagggcgg cgagcgcggt cgcgaagctc tccgcgaaga
     8881 cctccgagtc ctgcggcacg acccagtcgg gcgtggacga accaggctgg atgaccatcg
     8941 cagtcgagtc ttcgagcatg ggataggcaa cgagctcggc gttggccacg cgccagtccg
     9001 gcaccgcgaa cggcaggcga ttcttgagca ttgccagcac ccgcgcctct ggttcgacct
     9061 tcgcgcttac ctcggctcgg cgcgggatgc gcagcaccca ccgacgtcca tcgtcgacgg
     9121 tggcgatcac gatcctatag tcgagcccaa gctcattgac agtcagcggg ccatggagct
     9181 tgagcccatg tcgggctgca agtgcgtaca gttgggaggt atcggcggtc gtgactacgg
     9241 tcatgattca ctcctgaggg cttgacgggt ttagccacct aaatgtaaca gtcacgtcgg
     9301 ttatattcaa tc
//
LOCUS       DAEBXN010000149         9197 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.149, whole genome shotgun sequence.
ACCESSION   DAEBXN010000149 DAEBXN010000000
VERSION     DAEBXN010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9197
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.149"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..107
                     /locus_tag="KJL99_003767"
     CDS             <1..107
                     /locus_tag="KJL99_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBD4438340.1"
                     /translation="QHLARLVRKSLSFSKSVELHDKVIGHYLNIKHYQ"
     gene            124..1050
                     /locus_tag="KJL99_003768"
                     /pseudo
     CDS             124..1050
                     /locus_tag="KJL99_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226798.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            1302..2219
                     /gene="oxyR"
                     /locus_tag="KJL99_003769"
     CDS             1302..2219
                     /gene="oxyR"
                     /locus_tag="KJL99_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HBD4438341.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVLMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPCRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(2202..3602)
                     /gene="sthA"
                     /locus_tag="KJL99_003770"
     CDS             complement(2202..3602)
                     /gene="sthA"
                     /locus_tag="KJL99_003770"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HBD4438342.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIESCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            3923..4573
                     /gene="fabR"
                     /locus_tag="KJL99_003771"
     CDS             3923..4573
                     /gene="fabR"
                     /locus_tag="KJL99_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HBD4438343.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            4573..4932
                     /locus_tag="KJL99_003772"
     CDS             4573..4932
                     /locus_tag="KJL99_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HBD4438344.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMSVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4972..6072)
                     /gene="trmA"
                     /locus_tag="KJL99_003773"
     CDS             complement(4972..6072)
                     /gene="trmA"
                     /locus_tag="KJL99_003773"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HBD4438345.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQFETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      6186..6376
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6441..8285
                     /gene="btuB"
                     /locus_tag="KJL99_003774"
     CDS             6441..8285
                     /gene="btuB"
                     /locus_tag="KJL99_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HBD4438346.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTAIRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKALNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            8230..9087
                     /gene="murI"
                     /locus_tag="KJL99_003775"
     CDS             8230..9087
                     /gene="murI"
                     /locus_tag="KJL99_003775"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBD4438347.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2103 a         2224 c         2480 g         2390 t
ORIGIN      
        1 gacaacatct ggcaaggctg gtacggaagt cactgtcgtt ctcaaaatcg gtggagctgc
       61 atgacaaggt catcgggcat tatctgaaca taaaacacta tcagtaagtt ggagtcatta
      121 cccggtctgg gtattggcga agcgccattt atgcctgcgg gagtaaagtc gatcaccgac
      181 tggtatgcac aaaaagaacg cggcacggcg ctggggattt ttaactcatc taccgttatc
      241 ggtcaggcta tcgcgcctcc tgctcttgta ttgatgcagc tggcatgggg ctggcggacg
      301 atgttcgtta tcatcggcgt ggcagggatt ctggttggga tctgttggta cgcgtggtat
      361 cgcaaccggg cgcagtttgt cctgactgac gaagaacgga cgtatctctc cgcgtcggtt
      421 aaaccgcgtc cacagctgca atttagcgag tggctggcgc tgtttaagca tcggacaacc
      481 tgggggatga ttttaggttt ctctggcgtc aattataccg gttggcttta cattgcgtgg
      541 ttacccggtt atttgcaagc cgaacaaggt ttcagtctgg cgaaaaccgg ttgggtagcg
      601 gcgattcctt tcctcgcggc ggcggtcggg atgtgggtta acggtattgt ggtcgatcgg
      661 ctggcgaaaa aaggctacga cctggcgaag acgcgtaaaa cggctattgt ctgcggtttg
      721 atgatgtcgg cattaggcac gttgctggtc gtgcaatctt cctcgccagc tcaggcggta
      781 gcgtttatct caatggcgct gttctgcgtg catttcgcgg gaacgtctgc atggggactg
      841 gtgcaggtga tggtgtcaga aacaaaagtg gcttccatcg ccgggattca aaactttggc
      901 agttttgttt ttgcttcctt tgctccgatc gtaaccggtt gggtagtgga taccacacac
      961 tcgtttaatc tggcgctggt tatcgcggcc tgtgtgacgt tcaccggggc gctgtgttac
     1021 ttctttattg tcaaagatcg cattgagtaa tcgttactat caggctctct tgctgtgggc
     1081 ctgtagaata aaaaaaagcg gatcctggag atccgcaaaa gttcacgttg gctttagtta
     1141 ttcgagttga gaaactctcg aaacgggcag tgacttcaag ggttaaaaga ggtgccgctc
     1201 cgtttctgtg agcaattatc agtcagaatg cttgataggg ataatcgttc attgctattc
     1261 tacctatcgc catgaactat cgtggcgatg gaggatggat aatgaatatt cgtgatcttg
     1321 agtacctggt ggcattggct gaacaccgcc attttcggcg tgcggcagat tcctgccacg
     1381 ttagccagcc gacgcttagc ggccaaattc gtaagctgga agatgagctg ggcgtgatgt
     1441 tgctggagcg gaccagccgt aaagtgttgt tcacccaggc gggaatgctg ctggtggatc
     1501 aggcgcgtac cgtgctgcgt gaggtgaaag tccttaaaga gatggcaagc cagcagggtg
     1561 agacgatgtc cggaccgctg catattggtt tgattcctac ggttggacca tacctgctac
     1621 cgcatattat cccgatgctg caccagacat ttccaaagct ggaaatgtat ctgcatgaag
     1681 cacagaccca ccagttactg gcgcaactgg acagcggcaa actcgattgc gtgatcctcg
     1741 cgctggtgaa agagagcgaa gcattcattg aagtgccgtt gtttgatgag ccaatgttgc
     1801 tggctatcta tgaagatcac ccgtgggcga accgcgaatg cgtactgatg gccgatctgg
     1861 caggggaaaa actgctgatg ctggaagatg gtcactgttt gcgcgatcag gcaatgggtt
     1921 tctgctttga agccggggcg gatgaagata cacacttccg cgcgaccagc ctggaaactc
     1981 tgcgcaacat ggtggcggca ggtagcggga tcactttatt gccagcgctg gctgtgccgc
     2041 cggagcgcaa acgcgatggg gttgtttatc tgccatgcat taagccggaa ccatgccgca
     2101 ctattggcct ggtttatcgt cctggctcac cgctgcgcag ccgctatgag cagctggcag
     2161 aggccatccg cgcaagaatg gatggccatt tcgataaagt gttaaaacag gcggtttaaa
     2221 ccgtttaacg cagctacccg ataggcttcc gccatcgtcg ggtagttaaa ggtggtgttg
     2281 acgaagtact caatagtgtt gccgccacct ttctgttcca taatcgcctg accgatatga
     2341 ataatttcgg cagcgcgctc gccaaagcag tgaataccca gaatctcttt tgtttcccga
     2401 tggaacaaaa ttttcagcgt gcccacgttc atgccgacga tttgtgcacg tgccagatgt
     2461 ttgaactggg cgcggcccac ttcatacggc actttcatcg cggtcagctg ctgttcggtt
     2521 ttgcccacag agctgatttc cgggatggtg taaatgccgg tagggatatc ttcaatcaga
     2581 tgtgcggtgg cttcgccttt taccagcgcc tgcgcggcaa tgcgcccctg gtcataggcc
     2641 gctgatgcca ggctcggata accaatcacg tcgcccaccg cgtaaacgtg cggctgtgcg
     2701 gtctgataca tgctgttgac cttcagctgt ccgcgactgt cagtttccag cccaatgttc
     2761 tgtaacgcca gcgaatcggt attaccggta cgaccgttgg catagagcag gcagtcagct
     2821 ttcagttttt tacccgactt cagatgcatg atcacaccgt cgtcacagct ttcgatcttc
     2881 tcgtactctt cgttgtgacg aatcactacg ccactgttcc agaagtggta ggagagagaa
     2941 tctgacatct cttgatcgag aaatgccagc aggcgatcgc gggtgttgat cagatccact
     3001 tttacatcca taccgcggaa gatcgacgca tattcacagc cgatcactcc agcaccatag
     3061 ataagtacat ggcgcggttc gtggtgcatg ctaagaattg agtcgctgtc gtaaatgcgt
     3121 ggatgggtga aatcaacatc tgttggatga tatggacgag agccgcaggc aataacaaat
     3181 ttttcagcgg ttagtgtttc aacgctgccg tccgggcaat ccagcgccaa cgtatgctcg
     3241 tcaacaaagc gagcgttccc ctgcaatatt tcacagtgat tacgttcgta aaatccctga
     3301 cgcatgcgcg tttgttgatt aatcacgtta tcggcatggt taaggatatc ggcaaaagaa
     3361 gagcggagca gtcgggaatg gtcgctgtat agtgggtttt gattgaattc tataatgcgg
     3421 ctgacggcgt gacggagagc tttcgacggg atggtgcccc agtgggtgca accgccgcca
     3481 acattttgat aacgctcgat aactgcgacg cgcgcacctt gcttaaccag gcccattgca
     3541 gcgccttcgc cgccggggcc ggaacctatt actatggcat cgtaatcgta ggaatgtggc
     3601 atggtagggc ttacctgttc ttatacataa aagcaacaga atggtaacat tctatcgcgg
     3661 gtaagccaat tgatccccgt catttatctg gctatatcct gagcggcctt tgctttgtct
     3721 gtttcttact tttgccctga cgttttattg gatttttatc gacgatactc tccgtttaag
     3781 cggcaggttt ccgctgtacg taaaagaacc ggccagtaaa tatgttttat tgcgttaccg
     3841 ttcattcaca atactggagc aatccagtat gttcattctc tggtatagtg ccagcagtac
     3901 ttttggcaag gattcagaca tcgtgatggg cgtaagagcg caacaaaaag aaaaaacccg
     3961 ccgttcgctg gtggaagccg catttagcca attaagtgct gaacgcagct tcgccagcct
     4021 gagtttgcgt gaagtggcgc gtgaagcggg cattgctccc acctcttttt atcggcattt
     4081 ccgcgacgta gacgaactgg gtctgaccat ggttgatgag agcggtttaa tgctacgcca
     4141 actcatgcgc caggcgcgtc agcgtatcgc caaaggcggg agtgtgatcc gtacctcggt
     4201 ctccacattt atggagttca tcggtaataa tcctaacgcc ttccggttat tattgcggga
     4261 acgctccggc acctccgctg cgtttcgtgc cgccgttgcg cgtgaaattc agcacttcat
     4321 tgcggaactt gcggactatc tggaactcga aaaccatatg ccgcgtgcgt ttactgaagc
     4381 gcaagccgaa gcaatggtga caattgtctt cagtgcgggt gccgaggcgt tggacgtcgg
     4441 cgtcgaacaa cgtcggcaat tagaagagcg actggtactg caactgcgaa tgatttcgaa
     4501 aggggcttat tactggtatc gccgtgaaca agagaaaacc gcaattattc cgggaaatgt
     4561 gaaggacgag taatgaaaca agcaaatcaa gatagaggta cgctgctgct ggcgttggtt
     4621 gctggtttat cgattaatgg tactttcgca gcgctgttta gctccattgt gccattttct
     4681 gtattcccga ttatttccct ggtgctgacg gtttactgcc tgcatcaacg ttatcttaat
     4741 cgcaccatgt cggtaggctt gccgggtctg gcagctgcct gttttattct cggcgtactg
     4801 ctgtacagca cggtagttcg tgcggaatat ccggatatcg gctctaactt cttcccggca
     4861 gtactctccg tcattatggt gttctggatt ggcgcgaaga tgcgtaaccg taagcaggaa
     4921 gttgctgagt aatcggtatc atgccggatg ctggcgcatc cggcatgggt tttacttcgc
     4981 ggtcagtaat acgccgcact ccatatggtg cgtgtagggg aactgatcaa acagagccag
     5041 acgttcgacc ttgtgcgtct ggcttaatgt ttccagattt ttgcataacg tttccggatt
     5101 gcaggagata tacagaatac gtggatacgc ctgcaccatt ttctcggttt cactgtccag
     5161 accgctgcgc ggtgggtcga caaaaatggt ttcgaactga tagctcttta agtcgatccc
     5221 ttgcaggcgg ttaaactcgc gcacaccatt catcgcctga gtaaattctt ctgccgccat
     5281 acgaataatt tgtacgttat caatatggtt agcggcaatg ttgtattgcg cggcagcgac
     5341 tgacggtttg gcgatttcgg tggctaatac ccgatcaaaa ttgcgcgcca gcgctaatga
     5401 aaagttaccg ttgccgcagt acagctccag taaatcgcct tttgagcctt tggttacgtc
     5461 cagcgcccat tccagcatct gaatattcat cgccgcgttc ggctgggtaa agctgttttc
     5521 tacctgacga tagatcatct ctttccctgc gaccggcaga cgctcgtcga tgtaatcttg
     5581 atccagcgcg attttggttt tcgttgcccg accaatcaga tgcacattca gattctgcgc
     5641 gcgcagtgca tcgcgcagtg cttccgcttc ctgacgccac tcatcatcca gctttttatg
     5701 gtacagcagg gaaaccaccg cctgattact cagcgtggtg aggtaatcaa tctggaacaa
     5761 cttgtggcgc agaacgggat tattacgcac acccgcaatc atcgccgtca tcaactggtt
     5821 gatgagttca ctggcggcgg ggaagctatc cacgcggatg cggcttttgg tttgttgatc
     5881 gaaaatgatg tgatacaggt catcgccatc gtgccagatg cggaactccg cgcgcatccg
     5941 gtaatgactg accggcgagc gaaacacttc cggaaccagg tcagaaaacg gtgccatcat
     6001 actttgcaaa cgtaccactt tttcggctaa ctgcgcttca tactgttctg ttggaaggtg
     6061 ttcgggggtc atgatgtatc ctgaaaaatt aaagtacgcg gcgattgtag ggattgctca
     6121 tcagatgtcc agatcttgat gaattcctat ttgtgagcta cgtctggaca gtaacttgtt
     6181 acaacctgta gcatccactt gccggtcctg tgagttaata gggaatccag tgcgaatcta
     6241 gagctgacgc gcagcggtaa ggaaaggtgc gatgattgcg ttatgcggac actgccatcc
     6301 ggtgggaagt catcatctct tagtatctta gatacccctc caagcccgaa gacctgccgg
     6361 ccaacgtcgc atctggttct catcatcgcg taatattgat gaaacctgcg gcatccttct
     6421 tctattgtgg atgctttaca atgattaaaa aagcttcgct gctgacggcg tgttccgtca
     6481 cggcattttc cgcttgggca caggatacca gcccggatac tctcgtcgtt actgctatcc
     6541 gttttgaaca gccgcgcagc actgtgcttg caccaaccac cgttgtgacc cgtcaggata
     6601 tcgaccgctg gcagtcgacc tcggtcaatg atgtgctgcg ccgtcttccg ggcgtcgata
     6661 tcacccaaaa cggcggttca ggtcagctct catctatttt tattcgcggt acaaatgcca
     6721 gtcatgtgct ggtgttaatt gatggcgtcc gcctgaatct ggcgggggtg agtggttctg
     6781 ccgaccttag ccagttccct attgcgcttg tccagcgtat tgaatatatc cgtggaccgc
     6841 gctccgctgt ttatggttcc gatgcaatag gcggggtggt gaatatcatc acgacgcgcg
     6901 atgaacccgg aacggaaatt tcagcagggt ggggaagcaa tagttatcag aactatgatg
     6961 tctctacgca gcaacaactg ggggataaaa cacgggtaac gctgttgggc gattatgccc
     7021 atactcatgg ttatgatgtt gttgcctatg gtaataccgg aacgcaagcg cagacagata
     7081 acgatggttt tttaagtaaa acgctttatg gggcgctgga gcataacttt actgatgcct
     7141 ggagcggctt tgttcgcggc tatggctatg ataaccgtac caattatgac gcgtattatt
     7201 ctcccggttc accgttgctc gatacccgta aactctatag ccaaagttgg gacgccgggc
     7261 tgcgctataa cggcgaactg attaaatcac aactcattac cagctatagc catagcaaag
     7321 attacaacta cgatccccat tatggtcgtt atgattcgtc ggcgacgctc gatgagatga
     7381 agcaatacac cgtccagtgg gcaaacaatg tcatcgttgg tcacggtagt attggtgcgg
     7441 gtgtcgactg gcagaaacag actacgacgc cgggtacagg ttatgttgag gatggatatg
     7501 atcaacgtaa taccggcatc tatctgaccg ggctgcaaca agtcggcgat tttacctttg
     7561 aaggcgcagc acgcagcgac gataactcac agtttggtcg tcatggaacc tggcaaacca
     7621 gcgccggttg ggaattcatc gaaggttatc gcttcattgc ttcctacggg acatcttata
     7681 aggcactaaa tctggggcaa ctgtatggct tctacggaaa tccgaatctg gacccggaga
     7741 aaagcaaaca gtgggaaggc gcgtttgaag gcttaaccgc tggggtgaac tggcgtattt
     7801 ccggatatcg taacgatgtc agtgacttga tcgattatga tgatcacacc ctgaaatatt
     7861 acaacgaagg gaaagcgcgg attaagggtg tcgaggcgac agccaatttt gataccggac
     7921 cactgacgca tactgtgagt tatgattatg tcgatgcgcg caatgcgatt accgacacgc
     7981 cgttgttacg ccgtgctaaa cagcaggtga aataccagct cgactggcag ttgtatgact
     8041 tcgactgggg tattacttat cagtatttag gcactcgcta tgataaggat tactcatctt
     8101 atccttatca aaccgttaaa atgggcggtg tgagcttgtg ggatcttgcg gttgcgtatc
     8161 cggtcacctc tcacctgaca gttcgtggta aaatagccaa cctgttcgac aaagattatg
     8221 agacagtcta tggctaccaa actgcaggac gggaatacac cttgtctggc agctacacct
     8281 tctgaaccac gtcccaccgt gctggtgttt gactccggcg tcggtgggtt gtcggtctat
     8341 gacgagatcc ggcatctctt accggatctc cattacattt atgctttcga taacgtcgct
     8401 ttcccgtatg gcgaaaaaag cgaagcgttt attgttgagc gagtggtggc aattgtcacc
     8461 gcggtgcaag aacgttatcc ccttgcgctg gctgtggtcg cttgcaacac cgccagtacc
     8521 gtttcacttc ctgcattacg cgaaaagttc gacttcccgg ttgttggtgt cgtgccggcg
     8581 attaaacctg ctgcacgtct gacggcaaat ggcattgtcg gattactggc aacccgcgga
     8641 acagttaaac gttcttatac tcatgagctg atcgcgcgtt tcgctaatga atgccagata
     8701 gaaatgctgg gctcggcaga gatggttgag ttggctgaag cgaagctgca tggcgaagat
     8761 gtttctctgg atgcactaaa acgtatccta cgcccgtggt taagaatgaa agagccgcca
     8821 gataccgttg tattgggttg cacccatttc cctctactac aagaagaact gttacaagtg
     8881 ctgccagagg gaacccggct ggtggattct ggcgcagcga ttgctcgccg aacggcctgg
     8941 ttgttagaac atgaagcccc ggatgcaaaa tctgccgatg cgaatattgc cttttgtatg
     9001 gcaatgacgc cagaagctga acaattattg cccgttttac agcgttacgg cttcgaaacg
     9061 ctcgaaaaac tggcagtttt aggctgattt ggttgaatgt tgcgcggtcg gaaaattatt
     9121 ttaaatttcc tcttgtcagg ccggaataac tccctataat gcgccaccac tgacacggaa
     9181 caacggcaaa cacgccg
//
LOCUS       DAEBXN010000150         9170 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.150, whole genome shotgun sequence.
ACCESSION   DAEBXN010000150 DAEBXN010000000
VERSION     DAEBXN010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9170
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.150"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(227..2770)
                     /gene="mdoH"
                     /locus_tag="KJL99_003776"
     CDS             complement(227..2770)
                     /gene="mdoH"
                     /locus_tag="KJL99_003776"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HBD4438348.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(2763..4298)
                     /gene="mdoG"
                     /locus_tag="KJL99_003777"
     CDS             complement(2763..4298)
                     /gene="mdoG"
                     /locus_tag="KJL99_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HBD4438349.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPRGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKITEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            4692..5849
                     /gene="mdoC"
                     /locus_tag="KJL99_003778"
     CDS             4692..5849
                     /gene="mdoC"
                     /locus_tag="KJL99_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HBD4438350.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(5857..7338)
                     /gene="clsC"
                     /locus_tag="KJL99_003779"
     CDS             complement(5857..7338)
                     /gene="clsC"
                     /locus_tag="KJL99_003779"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HBD4438351.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEVILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(7280..7813)
                     /gene="ymdB"
                     /locus_tag="KJL99_003780"
     CDS             complement(7280..7813)
                     /gene="ymdB"
                     /locus_tag="KJL99_003780"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HBD4438352.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYSYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(7908..8219)
                     /locus_tag="KJL99_003781"
     CDS             complement(7908..8219)
                     /locus_tag="KJL99_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBD4438353.1"
                     /translation="MFRPFLDSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSIPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(8340..8672)
                     /gene="csgC"
                     /locus_tag="KJL99_003782"
     CDS             complement(8340..8672)
                     /gene="csgC"
                     /locus_tag="KJL99_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HBD4438354.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQALHLSQQ
                     WTPSSEKS"
     gene            complement(8731..9096)
                     /gene="csgA"
                     /locus_tag="KJL99_003783"
     CDS             complement(8731..9096)
                     /gene="csgA"
                     /locus_tag="KJL99_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HBD4438355.1"
                     /translation="MPVHLSNNSGPNSELNIYQYGGGNSALALQTDARNSDLTITQHG
                     GGNGADVGQGSDDSSIDLTQRGFGNSATLDQWNGKNSEMTVKQFGGGNGAAVDQTASN
                     SSVNVTQVGFGNNATAHQY"
BASE COUNT         2297 a         2368 c         2180 g         2325 t
ORIGIN      
        1 gagagtggta aaggagaggc cctggaaata ccccacagcc ccgaaaaaga gccagtcagt
       61 tgcttaactg actggcatta cggcataacg ccggtatttt tattggctcg ttgtttttgc
      121 cggatgtggc gtgaacgcct tatccgacct acaaaatctt gcaaaatcaa taaattgcag
      181 gaacgatgta ggcctgataa gcgtagcgca tcaggcaact acgtttttat tgcgaagccg
      241 catccggttt acgcaatgcc agtggattga gctttatccc ttcgtaataa ctcacccatg
      301 aagaatatct ttcaggggaa ttccagacgc ggaaatgcag acgggccatc gtcaccggat
      361 cgcttagcag caccaggcga cgatcgcgat tcagcttctc tggcgtctcg ttaagcgcct
      421 gttcaacgtg gcggtcacgg gcgatttcca gcaccttact ggcgcggtga cgcgctgtcg
      481 ccattgcggt tgccagagcg ttaaatgacg ggttaaacac tgcgtgcata aagccatcgt
      541 caagggagcg ttggcgattc atctcaagga accgatcggt atcaaccagc acctgcggcg
      601 gtgaatactc ttccgggatc aggaacagtt tccagcgttt ggtgcgcaga ccaacggtgg
      661 cacggctgga aatcaccgaa acaaacggtg acaggatcaa cgagaagaca atcggtgcca
      721 gccagaacag gaaacgcaga tccagccacg ccatcccaac agcccacact aaccccaaca
      781 gcagctgtga gccgtggcgt ttgaacgctt cgccccatga agtggagtca tcatcacgct
      841 gcggtgaatt ccacaccact tcccagccaa ggaacgcgct gacaacgaag accgtatgga
      901 acagcatgcg taccggagcc agcagcacgg aaaacagcac ttccagcagc aacgataatg
      961 taacgcgcca gaagccgccg tattctttcg ttcctttgca ccagataagc aaaatgctca
     1021 ataacttcgg caggaacaac agcaccatgg tcgaagcaaa aagtgcaatc gccagctcag
     1081 gacgccactg cggccacacc gggaacaact gccgtggttg caggaagtat tgcggttcgg
     1141 tcaacgcatg cactacctgc aatgcagtag agagcgcgag gaacataaac cacagcggag
     1201 cggagagata agacatcacg cccgtcagga acaccgcacg gtgaaccggg tgcataccct
     1261 taaccaggaa cagacggaag ttcatcaggt taccgtggca ccagcggcgg tcacgtttta
     1321 gctcatcaag caagttaggc ggcaattctt cataagaacc cgggagatcg taagcaatcc
     1381 agacccccca acctgcacgg cgcatcaacg ccgcttctac gaagtcatgt gacaggattg
     1441 aaccggcaaa ggaaccttcg cccggcagcg gagccagtgc gcagtgctcg ataaatggtt
     1501 tcacgcggat aatcgcgtta tgtccccagt agtgcgactc gccaagttgc cagaagtgca
     1561 aaccggctgt aaacagtggc ccatacacgc gggtcgcgaa ctgctgacag cgcgcataca
     1621 gcgtatccat accggacgct ttcggcgacg actgaatgat cccggcgttc gggttggctt
     1681 ccatcaagcg caccaggccg cataaacaat caccggtcat taccgagtca gcatccagca
     1741 ccaccatgta gctgtactgg ctgccccagc gacggcagaa gtcatcgata ttaccgcttt
     1801 tacgcttcac gcggcgacgg cggcggcgat agaaaatctg accttcgcca ccgacttcag
     1861 cgataagctc catccaggct ttttgctctg cgacgcagat atccgggtta taactgtcac
     1921 tcagaatgta gacatcaaag tgtttggcat tcccggtggc ttttactgat tcccacgttg
     1981 cacgcaagcc agcaaaaaca cggttcacgt cttcgttaca gataggcatg atcaacgccg
     2041 tgcgatgctc cgggtttaat ggttcatcgc caactgttga cgcagatata ctgtatttat
     2101 cgcgaccaat aagcagttgc aggaagccca tcaacgccgt ccagaatccg gcggacaccc
     2161 aacagaacag taccgcaaaa aggatcagga taccggtttg cagcatataa ggcagaagct
     2221 gcataaagga aacccacaaa tcctgaccaa ccatatccat aggattaatc agcgcccaac
     2281 cctgataagg aagaatggtc ttcatatacc aggtcgcgac aacagtttgc gcgagcgtca
     2341 ggatcaacag aatgtaacgg cggatggtac caacggtacg ccacttttgc tcgctctcct
     2401 gctcttcttt ggtcaaacga gccagatagc gcggcgtgac atcgcgtcca cgcaggcgat
     2461 cccagaaacg gcctaccggg ttggtacgcc acgggtcggg aaacatcgag gagcgttttg
     2521 cttctggcat cgccttaagc tgatcgcgcc cttcgtcgtc tttaattaac tgtccatcag
     2581 caagtgaatc tggccaggct tgttccagac gcgcctttac cgagccttgc ggggaatcat
     2641 cctcccgcgc ccaggtgcgg tgttcggcat ccagcgcctg atgaacggcg cggatatcag
     2701 tcttcggcaa tgctgctttc tcgcttgcgg cgatgggcat tgcgtcaatg tactcagttg
     2761 tcttattcat tggcaggtaa ctggtagctc caggtttcac tcaacgtctg atcggcattc
     2821 accagcgcag cacgcatttc agtgattttc ttggcatctt tcactttcac acgcatcacc
     2881 agacgccagc ctttggtaac cgggttatag cgcaccgtgc tttcaactat ctcaccatta
     2941 tcaccaatgc tggtttgcgc tgtgaccggg gtatcctctg gcagtttttt catctctgcg
     3001 ccggtaaaat cgaccacaaa ggcgatagta ccgtcaggct ggcgaatcag gttcgactgc
     3061 ttcacatccc ccgttgaacg acgcgtttgt tgcacccatg cgttatctgg cgcatgcagt
     3121 ttgtcttcat cacggctgaa ggtgatggtg tatttaaagt tcatctcttt acccggctcc
     3181 ggcagctgat ccggcgtcca gtaagcgacg atgttatcgt tggtttcatc gttggttgga
     3241 atttccacca gctcaacgct gcctttgccc cactcccctt tcggagtcac ccatgcgctt
     3301 ggacggagat cgtaacgatc atcgagatct tcaaagcggg agaaatcacg acctcgttgc
     3361 aacagaccaa agcctcgcgg gttttccatg gagaagctgc tgaccgctaa atgtttcggg
     3421 ttattcaacg gacgccagat ccattcgcca ttaccagcat ggatagacag accgttagag
     3481 tcgtgcaact ccggacgata gttatttgca ggcgacggtt ggttcggccc aaacaggaac
     3541 atactggtta acggtgcaac ccccagtttg ccgactttat cgcgcagata gattttcgac
     3601 tgcacatcca caaccgtgtc acgccctggc ataaccacga atttgtaagc acctgtcgca
     3661 cgcggcgagt caagcaatgc ataaatggtt aaacgtttat cagtcggttt tggacgctcg
     3721 atccagaact ctttgaagcg tggaaattct tcacccgatg gcaaagcggt atcaattgct
     3781 aggccgcggg cagaaaggcc ataaacctga cctgcaccaa tcacgcggaa atagctggcc
     3841 ccgagcatgc tgacgatttc atcgttttta tctttgctgt tgatcgggta aagcacttta
     3901 aaaccggcaa aaccaaggtc ttttaccgtg tctttgtcat gctgaacatc gccgaaagtg
     3961 aaataatccg ggctgtattt gattcgtttg actgcggtgg cagtcacttc atttattttg
     4021 accggggtat cgaagtacat accctgatgg tagaactcga gtttgaatgg ggtcttcaga
     4081 ttgttccagt acgctttgtc atgattaaac tggatctgct gatagtccgc gtatttcatg
     4141 tcgcggaaaa cggagggcaa gttgctttta ggcgcctcat agcctttccc ggctaaggat
     4201 tgagcttgct ttgcgacatc atcaatactg aaagcccagc ttgaagatgt atacagggtt
     4261 aacattactg cagcactcaa ccaacgcatt ttcatcattt gtagtttatg tttcataatt
     4321 agtaagcact tccccctttg tgtgcttata tcgatccgat ctattttaat ggataaccag
     4381 ccattccgac aactgaatcc cggtatcgtt cagcacgctg gctcatggat taagcacctg
     4441 attgcaaccg ttttcacttt ccgtgcttct cctaaagaca gaagcatcga cttcgtgtag
     4501 ggtttgccag caaatttatt tatcaaccgt cttacagacg cttccattct ggttagtggt
     4561 agcgatgatg ctatgcataa aaaccttgaa gcctgcgcct ggcgcggtca ggtcggtgtc
     4621 gctatggcaa caagcctaaa ggcataaccc gacagggata agacgaaaaa tccgagatta
     4681 gttaaccata tatgaatcca gtacccgcgc aacgtgaata tttcctcgac tccatccgcg
     4741 cctggctgat gttgttaggg atcccttttc atatttcttt aatctattcg agccatacat
     4801 ggcatgtgaa tagcgccgaa ccgtcattgt ggctaaccct ttttaatgac ttcatccact
     4861 cgttccgcat gcaggtattt ttcgttatat ccggatactt ttcctacatg ctttttttac
     4921 gctatccctt gaaaaaatgg tggaaagtac gtgtcgaacg tgtaggtatc ccgatgttaa
     4981 cagccatccc cctactgaca ttaccgcaat ttattatgct gcaatatgtc aaagggaaag
     5041 cggaaagttg gcctgggcta tcattgtatg acaaatataa tacgttggcc tgggaattaa
     5101 tatcacacct gtggttttta ctggtgttag tagtcatgac gacgctgtgt gtatggatat
     5161 ttaagcgcat cagaaataat ttagaaaatt ctgataaaac gaataaaaaa ttctcgatgg
     5221 taaaactatc ggtgattttt ttatgcctcg gcatcggcta tgcggtaata agaagaacga
     5281 tttttattgt gtatccaccc attctgagta atggcatgtt caattttatt gtcatgcaaa
     5341 cgctatttta tttaccgttc tttatcctcg gcgcactggc tttcattttc cctcatctta
     5401 aagccttgtt taccacgccg tctcgtggct gtaccctcgc tgcagcattg gcgtttgtcg
     5461 cttatttact caaccagcgc tatggcagtg gcgatgcctg gatgtacgaa accgagtcgg
     5521 tgatcaccat ggtcctcggc ctgtggatgg tgaatgtggt cttctccttt ggccaccgtt
     5581 tgcttaactt ccagtcagcg cgggtgactt attttgttaa cgcatcgctg tttatctatc
     5641 tggttcacca cccgttaacg ctgtttttcg gcgcatacat tacaccgcac atcacctcca
     5701 actggcttgg ttttctctgt ggcctgatat tcgtagtagg gattgcgata attctgtatg
     5761 aaattcattt acgcatcccg ttactgaagt ttttgttctc tggtaaaccg gttgttaagc
     5821 gtgagaacga taaagcacca gcccgttaag ccacatttac aataaccatt ccacgggcaa
     5881 tatcgacgcc agtctgacca taacccgctt ccagaaactg gtggcgggtt cttttttgag
     5941 aataacctct ttctttgcat gacgatcaac ccagttgatc cgtccccacc tgtccagacg
     6001 gagctgccag gccgcatcgt actggctctg aataaagcgt ttatcaatta actgtgccag
     6061 cgtttcgctc tctatcacga agcccatttc agtattgagc aatgttgaac gcggatcgaa
     6121 attgaaagag ccgataaaca ccgttttacc atcgatgcta aaagttttag catgcaggct
     6181 ggcaccggaa ttaccggtta tgccgcgatc gtgtaacgta ctgctttgtt cacgcgtcgg
     6241 cttgagttca tataattcca cgccatagcg gagcaatttt ttgcgccagc gcgcgtatcc
     6301 ggcatggacg acagcaacat cgttagcggc aagagaattg gttaggatcg caatctttac
     6361 cccttttctc accatccgta agagttgcgc cacacctgcg cgtgtcggta caaaatagga
     6421 agagataata tcgatgcgtt cactgggtga gcccatgata tcgaacaggc gctgcggtaa
     6481 cagtgaatgc cgttttgcct tgccctcccc tttcgccgga tcatcactta ataaacgtgt
     6541 cttcgcccag ataagcggca acgttccatc aaccagatga tttataaatg gactggattc
     6601 cattttgcgt aaataacgat gcgtcatggc atcgttatgc caggaggcgg gtaactcgat
     6661 gcgatccgcc atttcacctt ccgggacatc cagcacctgc tgtaagggtg aaaccgattt
     6721 gcaataccag tagcgggcga aatcatcggc aacatcctct accacggggc ctattgccat
     6781 gacatctaaa tccgaaaaaa gtggctcttc ccctgcacca aaataggcat caccaatatt
     6841 tcgtcctccc actagggtaa ccacgccatc gacagtgaaa cttttattgt gcatccggcg
     6901 attgagacgg gaaaagtcgg tgatatatcc aagcggacgt agcaagcgaa acgagaaagg
     6961 attaaaaagc cggacttcaa tgcgtggatg actgtcaagc aagcgcaaaa tgtcgtcaag
     7021 tccgggcgta ttgttgtcgt ccagcaacaa acggacgcga acgccacgct ttgctgcggc
     7081 taacagggcg gaaaacagta accgccccga catatcgtcc tgccagatgt aatactgaac
     7141 atccagcgta tgctctgcca tttcggccag acgataccgg gcggcaaacg catccagact
     7201 tttctccaga gggaaaagac cacactgacc gggatgttgc gaacacagtg gcagcaccgc
     7261 gctcgccagc cggggcaaat cattcatctc cttgttgggt aaggagtctt tcgtagaggt
     7321 gggcgttttc ttcatcataa cagacaaagt atacctgttc gggtaaagcg tgacgggtaa
     7381 taaattctga aacggtttta actgcgattt cagccgctgc cgcacgaggg taactataaa
     7441 ccccagtact gattgcagga aaagccactg acgtatagct gtttgccgcc accagtcgta
     7501 ggctattcag ataggcatcc tgcaaaagct ggtcttcgtt ttgttcccca ccacgccaga
     7561 ctggcccgac ggtgtgtact acggctttag cgggaagatc gcctgcaagc gtaataacgg
     7621 catgccccgt agggcaatcg ccctgctgtt gcctgacttt taaacaggca tccagcaggg
     7681 ccggacccgc tgcgcgatga atggccccat cgacgccgcc gcctcccatt aatgacggat
     7741 tagccgcatt cacaatcaca tcaacggcca gtttggtaat atcaccctgc acaacatgaa
     7801 tacgcgtttt cataacgcct ccttacacct tcttgtttct taagtgtaca gcaggctttt
     7861 tggttaaatc gcatttttct gctcagcaac gtgagttgtt cagaaactta gttatattcg
     7921 atgttcatta cagccagctt tttctgttta tcaagatact gcacttttac tgaggctatt
     7981 tgcgcgtttt tgacattgcc gctcattaac gctttgctgg agtaggtttt gcccggaata
     8041 gaaccattct gtgggcagtt catttctacg ggtgatgact gggtgctgac gtcacatgcc
     8101 ggttccacaa tttggccata aaaatggatc acgccccctt gcgcggtgct tcctgcaatg
     8161 gcaataaaag gaaaaaacaa actgccgagc ataagagagt ctaaaaaagg tctgaacatt
     8221 tattctattt cctcaatgat tagtcatcct tgaaggttgt gttatccata ctttcaggtt
     8281 ggctgattcg cagcagacca ttctctccag attcatctta tgctcgatat ttcaacaaat
     8341 taagactttt ctgaagaggg cgtccattgt tgtgataaat gaagtgcctg tccatcagaa
     8401 acagtaacaa ctattttcac ccgatcttcc ggggaaatat ttaaactcaa cttcgtcaaa
     8461 gcaatgggtt gattagcagg caatgagagg gtcttttctt gcttcgtctg actttgccct
     8521 gaactgcctt cgcgcaggga caatatttgt actctgcaca gacaagattg agtaagagta
     8581 acttcaggaa taatggtgta catatcccct tgctgggtcg tattaaaggt tatctggctg
     8641 gaaagtgccg caaggagtaa taacgcattc atattcttct cccgaaaaaa aacagggctt
     8701 gcgccctgtt tctgtaatac aaatgatgta ttaatactga tgagcggtcg cgttgttacc
     8761 aaagccaacc tgagtcacgt tgacggagga gttcgatgca gtctggtcaa ctgcagcacc
     8821 gttgccacca ccgaactgtt taacagtcat ttcagaattt ttgccgttcc actgatcaag
     8881 agtggcgctg ttaccgaagc cacgttgggt cagatcgatt gagctgtcat ctgagccctg
     8941 accaacatct gcaccattac caccgccatg ctgggtaata gtcaagtcag agttacgggc
     9001 atcagtttgc agagcaagtg cagagttacc gccaccgtac tggtaaatgt tcagctctga
     9061 atttgggccg ctattattac tgaggtgtac tggcaatagc ggacactacc atttgttctt
     9121 ttttaagcag ccatctgatg atatttttcc ctgaaggctg ccggggagat
//
LOCUS       DAEBXN010000151         9029 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.151, whole genome shotgun sequence.
ACCESSION   DAEBXN010000151 DAEBXN010000000
VERSION     DAEBXN010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9029
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.151"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(97..1473)
                     /gene="trkA"
                     /locus_tag="KJL99_003784"
     CDS             complement(97..1473)
                     /gene="trkA"
                     /locus_tag="KJL99_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HBD4438356.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="KJL99_003785"
     CDS             complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="KJL99_003785"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HBD4438357.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRRQEELLAEFNTRDARYLHPSWLLKRLQKAYPEQ
                     WQSIAEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRIYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNQPGAEEGDGFFYAKLIKK"
     gene            complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="KJL99_003786"
     CDS             complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="KJL99_003786"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HBD4438358.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEEGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINIHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(3792..4301)
                     /gene="def"
                     /locus_tag="KJL99_003787"
     CDS             complement(3792..4301)
                     /gene="def"
                     /locus_tag="KJL99_003787"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBD4438359.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            4466..5590
                     /gene="dprA"
                     /locus_tag="KJL99_003788"
     CDS             4466..5590
                     /gene="dprA"
                     /locus_tag="KJL99_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HBD4438360.1"
                     /translation="MVDIEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATCGVTITSGLARGIDGVVHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLFEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5562..6035
                     /locus_tag="KJL99_003789"
     CDS             5562..6035
                     /locus_tag="KJL99_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HBD4438361.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            6064..6606
                     /locus_tag="KJL99_003790"
     CDS             6064..6606
                     /locus_tag="KJL99_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HBD4438362.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPVCGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            6611..7183
                     /gene="tsaC"
                     /locus_tag="KJL99_003791"
     CDS             6611..7183
                     /gene="tsaC"
                     /locus_tag="KJL99_003791"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HBD4438363.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSCWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            7188..8006
                     /gene="aroE"
                     /locus_tag="KJL99_003792"
     CDS             7188..8006
                     /gene="aroE"
                     /locus_tag="KJL99_003792"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBD4438364.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            8003..8260
                     /locus_tag="KJL99_003793"
     CDS             8003..8260
                     /locus_tag="KJL99_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HBD4438365.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(8236..8790)
                     /locus_tag="KJL99_003794"
     CDS             complement(8236..8790)
                     /locus_tag="KJL99_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HBD4438366.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         2258 a         2192 c         2253 g         2326 t
ORIGIN      
        1 ctaacaaatg ttaagccatt ttcctgccag gaatgaattt aattaatcct tgataatcaa
       61 agggaaataa taaggcgtca ttagacgcct tattaattac aagaagaaag ggcttggctg
      121 gaagagtctt tcgacgtcgg taataaactt tttatctgtg aggaacataa ttacgtgatc
      181 gccttgctca atgcgcagat tgtcattggc aatcatcacg tcgtttccac gtaccaccgc
      241 tccaataatc gttcctggcg gtagcttgat ttcgtcaatg actctgccga caacgcgtga
      301 ggtgctttca tcaccgtgag caacggcttc aatagcttct gctacgccgc ggcgcaatga
      361 ggaaacacca acaatatctg cttttcgcac atggctaagc aacgcagaaa tagttgcttg
      421 ttgtggtgaa attgcaatat cgatcacgct cccctgaacc agatccacat aagcgcgacg
      481 ctggatcaat accatcacct ttttcgcacc catacgtttg gcaagcatgg cggacatgat
      541 attggcctcg tcatcgttgg tgacggcaat aaacagatca acttgatcga tatgttcttc
      601 ggccagcagt tcttgatccg acgcatcacc aaaaaagacg atcgtattct gcaacttttc
      661 cgccagttcg gcagcgcgct gctgattacg ttcgatgagt ttaacgctgt aatctttttc
      721 cagacgacgc gccagccctg caccgatatt accgccacca accagcatga tccgtttata
      781 cggtttttcc agtcgctgta attcactcat caccgcgcgg atatgctgtg aagcggcaat
      841 aaagaacact tcatcaccag cttcaacaat ggtcgaacct tgtggacgaa tggggcgatc
      901 gtggcggaaa attgctgcca cacgagtatc gatatgtggc atatgttcgc gcatggtcga
      961 aagtgcatta ccaatcagcg ggccgccata ataggcttta actacagcga ggctgacttt
     1021 accctcagcg aagttcacca cctgcaatgc gcctggatac tcaatcagtc ggtaaatatt
     1081 atcgataacc aactgctctg gtgcgatcag atgatcaatc ggtacagcat ctgaatgaaa
     1141 tagcttatcg gcatcgcgta cgtagtctgg tgagcggatg cgagcgatgc gattaggggt
     1201 gttgaaaagt gagtaggcta cctggcaggc aaccatattg gtttcatctg aactggttac
     1261 agcaaccagc atatcggcgt cgtcggcacc tgcctcccgc aatacgcgtg gatgagagcc
     1321 atgcccctgc acgactcgca ggtcaaattt atcctgcaag gtccgcagac gctcaccgtt
     1381 ggtatcgaca acagtaatat cgttgttctc gccaaccagg ttttccgcca gtgtgccgcc
     1441 aacctggccg gcacccagaa tgataatttt catcagtcgc gacccgttat ctcatcactt
     1501 tttgattagc ttagcgtaaa agaagccgtc gccctcttcg gcaccaggct gattttgttt
     1561 acccggttgc tctggtgttc ctgtttcgca aagttcggca tcagcggtac gttgcaaaaa
     1621 ggctttaatc tgcaggctat tctcttccgg taacaccgaa caggtggcat agaccagggt
     1681 tccaccggat tttaaatgcg gccaaatggc gtcgagaatt tcagactgca attgcgcgag
     1741 ttccgggata tcgcgatcgc gacgtaacca tttaatatct ggatggcgac gaatcacccc
     1801 cgttgctgaa caaggcgcat ctaataaaat gcgatcaaac tgttgctcgc cacaccattg
     1861 ggaagggtaa cggccatcac cttgtttcac ggttgccttc ataccaaggc gttttaaatt
     1921 gtcgtaaatg cgagagaggc gctgttcgtc aatatcaacc gcaacaacct gcgcttctgg
     1981 tgccacctca aggatatgcg ttgttttacc gccgggggcg gcacaaagat ccaagatgtg
     2041 ttcaccgttt tgtggcgcaa gccaggtcat gcaaccttgt gctgatgcat cctgaacggt
     2101 aacccatccg tcttcaaaac caggtagcgc atgaacaggt gcaggtgttt ccagccgtac
     2161 agcatcaggg taatccgcat gcgggaaacc tttcattcct gcttcatcca gcaatgcaag
     2221 ccagctgtcg cgggaatgat gcgtacgatt aatacgcagc cacattggcg gacgctggtt
     2281 attggcttcg gcgatggatt gccactgctc tggatacgct ttttgcagac gcttcagcaa
     2341 ccaggaagga tgcagataac gtgcatcacg ggtattaaac tcggctaata actcttcttg
     2401 ccgacgctgg aactggcgta atacaccgtt aatcaaccct ttaagttgcg gacgcttaat
     2461 tgcgatagcg ccttcaaccg tttcagccag cgcagcatga ggtggaatgc gggtataaag
     2521 cagttgatac aaaccaacca taatcaggta atgcacagtg cgctgtttgc cggtcatcgg
     2581 acgggccatt aacttattaa tcagccagtc taactgcgaa agcgtacgca gtacgccaaa
     2641 gcacaactct tgaagaagtg ctttgtcttt atcggaaact ttttgctgga gcggtggcag
     2701 aatgttgctt aatgattgcc cttgctcgac gacttgttca acggcctggg ccgccatgct
     2761 acgtaaatta cgttgttttt tcataagtat aaaaatgctc ggcaagaccg ggcttagaag
     2821 agtggactat cagaccagac ggttgcccgg aacaaaccat tcccgacgag agttcaggag
     2881 gtcttgcgcg ctcatcgctt tcttacccgc aggttgtaac gagagcaggt tcaggatgcc
     2941 atcaccagtc gcaacctgaa tgccttgttt gttggcttca aggatcgttc ctggtgcagc
     3001 gttggttgcc gtatcaatga ccgacgcttt ccagacttta accggctgtc cttcaatttc
     3061 cagccagctc attggccatg ggttgaaagc gcgaatgcag cgttcaagct gtgctgccga
     3121 aagtgaccag tcaatacgcg cttcttcttt actcaacttc tcggcgtaag tgacaagagt
     3181 ttcgtcctga acttctggtt tcgccgtgcc gtctgccaat tgtttcaacg tggtgataag
     3241 cccttgtggg ccaagctctg ccagcttgtc gtacagcgta ccactggtat cttctgcagt
     3301 aatcgggcag gagagcttat agagcatgtc accggtgtct aaaccaacat ccatttgcat
     3361 aatggttaca ccagtttctg catcacccgc ccatagtgag cgttggattg gtgcagcacc
     3421 gcgccagcgt ggcagcagtg aaccatgaat gttgatacag ccaagacgcg gcatctccag
     3481 cacagcttta ggcagaatta aaccataggc gacgacgacc ataacatcag cctgcaaatc
     3541 ggcgaccagt tgctggtttt cttgtggacg cagggaaaca ggttgaaaaa cgggcagacc
     3601 ttcttcctca gccagaactt taatcgggct gggcatcagt tttttaccgc gtcctgccgg
     3661 tcggtctggc tgggtgaaca cgccaacgac gttatgacca gaagacaaca gcgcgtcgag
     3721 atgacgcgct gcaaagtcag gtgtacccgc aaaaataata cgtagtgatt ctgacacgtt
     3781 agttcttatc cttaagcccg ggctttcaga cgatccagtt tttcaacttt ctgacgaatt
     3841 cgttgttgtt tcagcggtga cagataatcc ataaacagtt tgccaaccag gtgatccatc
     3901 tcatgctgaa tacagatagc taacagaccc tctgcttcca gttcaaatgg tttaccgtcg
     3961 cggtcaaggg cgcgaatttt aactttctct gcgcgcggca ctaaagcacg ttgttcaggg
     4021 atcgacaggc aaccttcttc aatgcctgtt tcgccgcttt tttctaaaag ctccggattg
     4081 attaacacca gccgttcgtc acggttttcc gaaacatcaa taacaatgat acgttgatgg
     4141 atatcaacct gggttgccgc caggccaata ccttcttctg cgtacatcgt ctcgaacata
     4201 tcatcgacga tacgctgaat ttctgcattc acttcttcta ccggtttagc aactttgcga
     4261 agccgctcgt ccggaatatg taacacttgc aaaactgaca taaatctcca gagatgtgtt
     4321 caggagttag aaagattatt tcttctattc tagacaaatc cccctctgat tgacagcatc
     4381 acttgattga cagcatcact tgattgacag catcactgac caatcgcaaa gattgctaag
     4441 gcttgcttat ggcagggaga taaggatggt cgatatagaa atttggctgc gtttaatgag
     4501 catcagcagc ttgtacggcg atgatatggt ccgtatcgct cactggctgg caaaacagtc
     4561 gcatattgat gcggttgtat tgcagcaaac agggcttaca ttgcggcagg cacaacgctt
     4621 tctttcattt ccacgaaaga gtatcgaaag ctcactttgt tggttggagc aacccaacca
     4681 tcatttaatc cctgcggaca gcgaatttta tcctcctcaa cttcaggcga cgacagatta
     4741 ccccggcgca ctgtttgttg aaggagaact gcacgcgctg cattcatttc agcttgccgt
     4801 agtggggagt cgggcgcatt catggtatgg cgagcgatgg gggcgattat tttgcgaaac
     4861 tctggcgacg tgtggagtga caattacgag tggactggcg cgtggaatcg atggtgtggt
     4921 gcataaagcg gccttacagg taaatggcgt cagcattgct gtattgggga atggacttaa
     4981 taccattcat ccgcgtcgcc atgcccgact ggctgccagt ctgtttgaac agggtggcgc
     5041 tctcgtctcg gaatttcccc tcgatgttcc accccttgct tacaatttcc cacgaagaaa
     5101 tcgcattatc agtggtctaa gtaaaggtgt actggtggtg gaagcggctt tgcgcagtgg
     5161 ttcgctggtg acagcacgtt gtgcgcttga gcaggggcgt gaagtttttg ccttgccagg
     5221 tccaataggg aatccgggaa gcgaagggcc tcactggtta ataaaacaag gtgcgattct
     5281 tgtgacggaa ccggaagaaa ttctggaaaa cttgcaattt ggattgcact ggttgccaga
     5341 cgcccctgaa aattcatttt attcaccaga tcagcaagac gtggcattgc catttcctga
     5401 gctcctggct aacgtaggag atgaggtaac acctgttgac gtcgtcgctg aacgtgccgg
     5461 ccaacctgtg ccagaggtag ttactcaact actcgaactg gagttagcag gatggatcgc
     5521 agctgtaccc ggcggctatg tccgattgag gagggcatgc catgttcgac gtactaatgt
     5581 atttgtttga aacctatatt cacactgaag ctgagttgcg tgtggatcaa gacaaacttg
     5641 aacaggatct taccgacgca ggttttgagc gagaagatat ctacaatgcc ctgctatggc
     5701 tggaaaaact tgctgattat caggaagggt tggcagaacc gatgcaactg gcctcggatc
     5761 ctctctccat gcgtatttat acaccggaag agtgtgaacg actggatgcc agctgccgtg
     5821 ggtttctgct tttccttgag cagattcagg tgctcaacct tgaaactcgt gaaatggtga
     5881 tagagcgagt gctcgcgctg gataacgcag agttcgaact ggatgatctg aaatgggtga
     5941 tcctgatggt gttgttcaac atcccgggct gcgaaaatgc gtaccagcaa atggaagaat
     6001 tactctttga agtgaatgaa ggtatgctgc attaatcatt tctttaattc agcataagtt
     6061 gttatggcaa aatcagcact gttcacggtg cgtaataatg agtcctgccc aaagtgcggg
     6121 gctgaactgg ttattcgatc cgggaaacac ggtccgtttc ttggatgctc acagtatccg
     6181 gcgtgtgact acgtccgtcc tctgaaatct tcagcggatg gacatattgt caaagttctg
     6241 gaggggcagg tttgccccgt atgtggcgca aatctggtat tacgccaggg acgctttggt
     6301 atgtttattg gttgcagtaa ctaccctgaa tgcgaacata ccgaacttat cgataaaccg
     6361 gacgaaacag caattacatg cccccaatgt cggacgggcc atctggtcca gcgccgctcc
     6421 cgttatggca aaacatttca ctcctgtgat cgctacccgg agtgtcaatt tgccattaac
     6481 ttcaaaccaa tagctggaga atgccctgag tgtcattatc cgctactcat cgaaaagaaa
     6541 accgcgcagg gtgtaaaaca cttttgtgcc agtaaacaat gtggaaagcc ggtttcggcg
     6601 gaataataac gtgaataata acctgcaagg agacgctatc gcagctgcga tagatgtcct
     6661 caatgaaaaa cgtgtcatcg cctatccaac ggaagccgtt ttcggtgtcg ggtgcgatcc
     6721 tgatagcgaa acagcagtga tgagactgtt ggagttaaaa cagcgtccgg ttgataaggg
     6781 gctgatttta atcgcggcaa attacgagca gcttaaaccc tatattgatg acaccatgtt
     6841 gactgacgtg cagcgtgaaa ccattttttc ctgctggcca ggtcctgtca cctttgtctt
     6901 tcccgcgcct gcgacaacac cgcgctggtt gacgggccgc tttgattcgc ttgctgtacg
     6961 agtcaccgac catccgttgg tggttgcttt gtgccaggct tatggtaaac cgctggtttc
     7021 taccagtgcc aacttgagtg gattgccacc ttgtcgaaca gtagacgaag ttcgcgcaca
     7081 atttggcgcg gcgttcccgg ttgtgcctgg tgaaacgggg gggcgtttaa atccttcaga
     7141 aatccgcgat gccctgacgg gtgaactgtt tcgacagggg taacataatg gaaacctatg
     7201 ctgtttttgg taatccgata gcccacagca aatcgccatt cattcatcag caatttgctc
     7261 agcaactgaa tattgaacat ccctatgggc gcgtgttggc acccatcaat gatttcatca
     7321 acacactgaa cgctttcttt cgtgctggtg gtaaaggtgc gaatgtgacg gtgcctttta
     7381 aagaagaggc ttttgccaga gcggatgagc ttactgaacg ggcagcgttg gctggtgcag
     7441 ttaataccct caagcggtta gaagatggac gcctgctggg tgacaatacc gatggtgtag
     7501 gcttgttaag cgatctggaa cgtctgtctt ttatccgccc tggtttacgt attctgctta
     7561 tcggcgctgg tggagcatct cgcggcgtac tactgccact tctttccctg gactgtgcgg
     7621 tgacaatcac taatcggacg gtatcccgcg cggaagagtt ggctaaattg tttgcgcaca
     7681 ctggcagtat tcaggcgttg ggtatggacg aactggaagg tcatgagttt gatctcatta
     7741 ttaatgcaac atccagtggc atcagtggtg atattccggc gatcccgtca tcgctcattc
     7801 atccaggcat ttattgctat gacatgttct atcagaaagg aaaaactcct tttctggcat
     7861 ggtgtgagca gcgaggctca aagcgtaatg ctgatggttt aggaatgctg gtggcacagg
     7921 cggctcatgc ctttcttctc tggcacggtg ttctgcctga cgtagaacca gttataaagc
     7981 aattgcagga ggaattgtcc gcgtgaatca ggccatccag tttccggaca gggaagagtg
     8041 ggacgagaat aaaaaatgtg tatgttttcc cgctctcgtg aatggtatgc aactgacatg
     8101 cgcgatctct ggcgagagtc tggcgtatcg ctttactgga gatacgccag aacagtggtt
     8161 agcgagtttt cgtcagcatc gctgggacct ggaagaagaa gcggaaaact taattcagga
     8221 acaaagtgaa gatgatcaag gctgggtctg gttaccctga tccagatatt cgtccttcca
     8281 tttcacgtaa ttattcgcgg aatagcgtaa cccagccttc tcttcatcac ttaacgggcg
     8341 gatctgtttg acggggctac cgagatacag atatccgctc tccagccgtt tattttgtgg
     8401 gaccagacta cccgcaccaa tcatcacatc atcttctact attgcgccat caagtaaaat
     8461 tgagcccatc ccaaccaaaa ctcgattgcc aatggtgcag ccgtggagca tcaccttgtg
     8521 accaacagtg acatcttcgc caatggttaa tgggttgcca tctgggttgt acgaggattt
     8581 atgagtgaca tgcaacatac tgccatcctg gatattggtg cgtgctccga tctgtacata
     8641 atgtacatct ccacgaatca caacgagcgg ccagatcccc acatcatcag ccagacgaac
     8701 gtcaccaatc acgacactgc tatcgtcgat cattacgcgc tgaccgattt gtggaaaaag
     8761 atcgcggtat gggcgtaaaa catcagacat acttacctca gcaataaatg atttactaat
     8821 gactttgggg gcattattgg ccttgtgcaa gtcttttagt atgcaaaaaa gcaccgtttt
     8881 gtgtgcgatt gcagcaaaaa gggtgaaaaa acaacaaaca gaaaagaaga tcaaaaaaat
     8941 acttgtgcaa aatattggga tccctataat gcgcctccgt tgagacgaca acgtgaaaca
     9001 cttcacagga tggtcggaac aacgaagag
//
LOCUS       DAEBXN010000152         9017 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.152, whole genome shotgun sequence.
ACCESSION   DAEBXN010000152 DAEBXN010000000
VERSION     DAEBXN010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9017
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.152"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(12..705)
                     /gene="ospZ"
                     /locus_tag="KJL99_003795"
                     /pseudo
     CDS             complement(12..705)
                     /gene="ospZ"
                     /locus_tag="KJL99_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase OspZ"
     gene            761..913
                     /locus_tag="KJL99_003796"
                     /pseudo
     CDS             761..913
                     /locus_tag="KJL99_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2294..3241
                     /gene="icsP"
                     /locus_tag="KJL99_003797"
     CDS             2294..3241
                     /gene="icsP"
                     /locus_tag="KJL99_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HBD4438367.1"
                     /translation="MKLKFFVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGSRNKKGAHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVLSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
     gene            complement(3803..4175)
                     /locus_tag="KJL99_003798"
                     /pseudo
     CDS             complement(3803..4175)
                     /locus_tag="KJL99_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4419..4538
                     /locus_tag="KJL99_003799"
                     /pseudo
     CDS             4419..4538
                     /locus_tag="KJL99_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            4788..5654
                     /gene="ospB"
                     /locus_tag="KJL99_003800"
     CDS             4788..5654
                     /gene="ospB"
                     /locus_tag="KJL99_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HBD4438368.1"
                     /translation="MNLDGVRPYCRIVNKKNESISDIAFAHIIKRVKNSSCTHPKAAL
                     VFLGEKGFCDSNDVLSIMGQQIPRVFKNKMLYDYVFKNEKSKNDFLKMAESWLPQSEP
                     IVINNDDDALNAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSHQLGLGSELID
                     VQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILNSLPFFKEKES
                     LLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSIPADPEHTVKRSS
                     QKKTFIINKELD"
     gene            5983..6723
                     /gene="phoN2"
                     /locus_tag="KJL99_003801"
     CDS             5983..6723
                     /gene="phoN2"
                     /locus_tag="KJL99_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family phosphatase PhoN2"
                     /protein_id="HBD4438369.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAENSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDEKMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
     gene            complement(7083..8531)
                     /gene="ospC4"
                     /locus_tag="KJL99_003802"
     CDS             complement(7083..8531)
                     /gene="ospC4"
                     /locus_tag="KJL99_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_156647404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
                     /protein_id="HBD4438370.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKTNPNKATKALQKNVLRVTNSSS
                     SGISEKHLDHCANTVKNFLRKSIAAQSYSKMFSQGTSFKSLNLSLEAPSGARSSFRSL
                     EHLDKVSRHYISEIIQKVHPLSSDERHLLSIIINSNFNFRHQSNSNLSNNILNIKSFD
                     KIQSENIQTHKNTYSEDIKEISNHDFVFFGVEISNHQEKLPLNKTHHTVDFGANAYII
                     DHDSPYGYMTLTDHFDNAIPPVFYHEHQSFFLDNFKEVVDEVSRYVHGNQGKTDVPIF
                     NTKDMRLGIGLHLIDFIRKSKDQGFREFCYNKNIDPVSLDRIINFVFQLEYHIPRMLS
                     TDNFKKIKLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSLGNKKADIALYLLSK
                     FNFTKQDVAEMEKMKNNRYCNLYDVEYLLSKDGANYKVLEYFINNGLVDVNKKFQKAN
                     SGDTMLDNAMKSKDSKMIDFFIKKWSGIRQTI"
BASE COUNT         2796 a         1675 c         1686 g         2860 t
ORIGIN      
        1 attaatatac attaagtaac aggcattcga gccttgattt gagaaagcct gtcattcatt
       61 tgttttatgt agctatctat ttcctctgat gcataactaa aaagttctcc catttcacga
      121 gcaataataa tctcagattt aatagacttt aatctctggc gactcatctc ttaacacttg
      181 cataatctct ctttttatat catatagttc tataaaccga tttgcccgta gttctccttg
      241 ggaagaatca tttctgaata caacaattat atacaaaaaa tcactatttt ctttagagaa
      301 gatcttacaa ctaatctctg ggagctcaca taatgcatgt aagtcaggat ggtacaaact
      361 atccacagaa aattgcccca taggacgttt tccagatata actgtcaacc agcttccttc
      421 cggtgtactg gcttcggctc tggaaatagg ctgaaaatct tccggacatg gagaatacat
      481 tgtcttcata taattaagtc gtctggattt ctcattaata aactcctcta tttttaattg
      541 tgccatactt ctggttatac cagagatata cttcttgtca tatatgtttg gtgattcatt
      601 aaagtaacca tgttcaactc taggataaat atttctcact atatattcag tatttgcagt
      661 atttattgga aatacgttct taatattctt gatgggacta atcattttaa tctctatact
      721 caaatataaa cattaccatg aacaatataa ttgttcctaa gtggaatgtc tccacggaga
      781 acactttatc tatcgggaaa tagtgcgggc aacagtgttt aattatatcg agtgcaatta
      841 caatcgctgg cgacggcacc gttggtgtgg cggcctcagt ccggaacaat ttgaaaacca
      901 gaacctcgct taggcctgtg tccatattac gtgggtaggc tcacagagcc tcacttacga
      961 ctgcgtccat attatgtggg taggatcaat aaagacgttt tatatgtgtc tgcttgcagc
     1021 cagaaaattt ataccctgca ggtatcataa acatgcttga taacttaatt ggggctccct
     1081 cttcctttta tggtgtaatt gtttgattga atacttccgg ggatttcagt atgaaatgaa
     1141 gtatatttaa tatactttac aaaaagtttg tattagtgga ataaggacaa tagcttatcc
     1201 aaactgccaa gcctctttat tataagtaag atctggcact ggaatattct caaaaggact
     1261 aacatcatca ccacgacctt caaccaacca ttttattgca tttaaatccc agaatagttc
     1321 agtagtggtc aatttgggaa acgcacaata ttgtacggtt acgttatcgc aatagagtga
     1381 actctcgccc tatacggcgt tttcaaggat tagtccttaa tcggacaacc acattaaagc
     1441 ataataaata attaagcttt aataatatac tatagataca gttctattat aaaaaatact
     1501 attttaggtg atgcattaaa ttagtagaat atttattctt ttataaaagc accaatgaat
     1561 tgtccaagta aacatgagat aaaatgtgta gaggataact gagtttggtc actttaactg
     1621 tattagtcgc agtctgactc tcgactttaa aaggatggga attacctgta ctgatagtga
     1681 gaaagctttc cattgaaata agtcaaataa aagttgatgg cttcagtgct aaagtgaggt
     1741 gcccgctagc cgtattcact cgaatcggaa ttcaatgtat tgcttctgcc atttccataa
     1801 tatttcacgg gaaaaacaaa atctgaaaaa ctatacttaa gtgttgtatt gagtcagttg
     1861 agatacaaat acatatttat cagcacacgt tacgtcaaat acaggaggtt gtcatgttgg
     1921 tactgaaagg cacgttggct attgcagccg tgatgacaat ttattgtctt atcgttgttc
     1981 ttatggaacg cttttctgat tgatttcata ttggcgagag aacaagaagg tgagtagaat
     2041 tgtcgcggaa tcctgaaact atcagcctca ggtatgaaca ccaaaaggta ggtagagaaa
     2101 tctatggccc ccgttacttt attcgcacaa tgaggtattt ttttattttt acaaaagttc
     2161 aataaatatt ataataaaag atatgttctt ggtaacataa acaaatattt aattaaaata
     2221 atgcttggca ttgacgttta tcttataaag taagaagatc atggacattt caaccaaaaa
     2281 agtagagttc tcgatgaaat taaaattctt tgtacttgca ctttgtgtac ctgcgatctt
     2341 tactacacat gctaccacta actatccact tttcataccg gacaacatca gtacggatat
     2401 tagtctagga tctctgagtg gcaaaacaaa agaacgcgtt tatcatccca aggaaggggg
     2461 gagaaaaatt agtcaactgg actggaaata cagtaatgcg actattgtta gaggtggcat
     2521 cgattggaag ctaattccaa aagtgtcttt cggagtttcc ggttggacta ctttaggtaa
     2581 ccagaaagca agcatggttg ataaagactg gaacaattcc aatacccctc aggtatggac
     2641 cgaccagagc tggcatccca atacgcatct ccgtgatgct aacgaattcg agctgaatct
     2701 taaaggttgg ttattaaata atttggatta tcgacttgga ctaatagcag gttaccaaga
     2761 gagtcgttac agttttaatg caatgggagg gagttatatt tatagtgaaa acggggggag
     2821 caggaataaa aaaggggcac atcctagtgg tgaaagaaca ataggttaca aacagctctt
     2881 caaaatacct tatattggat taactgctaa ttaccgccat gagaattttg agtttggagc
     2941 agaactgaaa tatagtggtt gggttctttc atctgataca gataaacact atcagactga
     3001 gacaattttt aaagatgaaa taaaaaacca aaattactgc tctgttgctg cgaatattgg
     3061 atactatgtc acccccagtg caaaatttta tatagaaggc tccagaaatt acatttctaa
     3121 taaaaaaggt gatacatctc tttatgagca aagtaccaat atatctggca ccattaaaaa
     3181 tagtgcaagt attgaatata ttggttttct cacttccgca ggtataaagt atattttttg
     3241 aattggttca ccgagttgat tacgttttca tggctaaaaa ttgccgccca ttgtgagtca
     3301 tattcgccct gactttccag aaccatacga actgcccgtt gacggacttc gggggaaaaa
     3361 cgtgtatttt tagtcattct gtttacctct ttctcagggc gtttagtctc caggatttcc
     3421 ggggcggttc aaaaaactct tcgttgatta ctgtggactt actgttggca ttaccgaccc
     3481 tgagaccgga gaaataagaa ccgcttagat catcgtggct gttctcgggg tatcaagtta
     3541 cacatgggca gaggctaccc aaggtctact attatcgcct gaaagcgctg atggaacagt
     3601 gctatcaggg acatgctgat gaaaatacct aaataatcag ccagcactct atctttccaa
     3661 atccacagca tagcaaagag agcaaaagag cctgtaaatt cagaaattaa ggaaacgcct
     3721 cttggtgtac ataatctatc ttatgttaaa tgaaatctaa tttcttgatt atatgctgga
     3781 tcgttcgtgg gtggctttgc ccctatattt ccagacacct gttatcattg gcccgctgcc
     3841 gcagatattc ccgtggcgag cgataatcca gcgcactatg cggatgccat tcgttataat
     3901 gctcgaatgc ctctgcaagg ttctttgctg ccgttaaccc gtcaggtttg ggcatgatac
     3961 tgatgtagtc acactttatt gttttcacga agctctttgc tattctgtta ctctccggac
     4021 tccgcaccgc cgtgttcttc ggttcaacaa agtcccaaca tccgggtgaa ctggcgtgtt
     4081 tcattagctc ggtagcatga accattatcc gtcagtcact tcactggaga cgccggaagc
     4141 tcgttgccga agcggtgttc caccgctccc agcatgtcgc caacgggaga gcatctacgg
     4201 tctgattgaa ctgtttattc aaattgtcca ccgtctgtca gtacgctctg aacgtcggct
     4261 ggttaaaaca ctgctcgctg attttcagag ggttcacggt aaaacagcac ttttattccg
     4321 gattgcggaa gcggctctga ataatccgga tgggctggtt aaagaggttg tgtaccatct
     4381 gcatattgtt gaaccagatc gcagcggtaa gagaagttca tcatacttgg cgcagttacg
     4441 cgatgtttct gctagaggtg atgccgtaaa gaacggcaga acattaccgg aacaagatag
     4501 cggtctacct gcactggtat cagacccggg gcttccccgg atgatctcca cggtacttta
     4561 ggtactgtgt ctatattatg taggtaggat caattaactt tgtagttgaa attctttcat
     4621 tgaaataatg aataaaaaag acaaaccgca ttaaacaatg ccagaatata attgcatttt
     4681 tgttccgttt ttttaaatca tagtatacaa agaagcttgc atactatatg aaaatataac
     4741 accctttact atgatgttta taaaacaata tatggagtca tgtaggtatg aatttagatg
     4801 gtgttagacc atactgtaga atagtcaata aaaagaatga aagcatatca gatattgcat
     4861 ttgcacatat aataaaaagg gtaaaaaatt catcatgtac tcacccaaaa gcagcattgg
     4921 tttttttagg agagaaaggt ttttgtgata gcaatgatgt tctatctatt atgggacaac
     4981 aaataccaag agtatttaag aacaagatgt tatatgatta tgtttttaaa aatgaaaaaa
     5041 gtaaaaatga ttttctaaaa atggctgaat catggctacc acagagtgaa ccaatagtaa
     5101 taaataatga tgatgacgca ttgaatgctg ctgcttattt ttctgtaaaa aaagcgaaaa
     5161 taaaaacagt aaacgatact gattttaaag agtataataa ggtttatatt cttgggcacg
     5221 gtagtcctgg ttctcatcaa ttaggccttg gttcggaact tattgatgta caaacaatca
     5281 tttcaagaat gaaagactgt ggtattctaa atgtgaaaga tatccgtttt acttcatgcg
     5341 gctccgctga taaagtggct cctaaaaatt ttaacaatgc ccctgctgaa agtctttctt
     5401 gtatccttaa ctctctgcct ttttttaagg aaaaagaatc tttgctagag cagataaaaa
     5461 aacaccttga aaacgatgag tcattgagtg atggtctaaa aatatccggc tatcatggat
     5521 atggagttca ctatggtcaa gagctttttc cctactcaca ttatcgttca acttcaattc
     5581 ctgctgatcc ggagcataca gtaaaaagaa gctctcagaa aaagactttt attattaata
     5641 aagaactgga ttagtataaa atttttaacc tatagttaat tgtttgcatt taagatgaag
     5701 ttattttatc tttagtagaa aagtgcttcc ctatgaggga aaatcacaaa tcatcataat
     5761 acagagacaa aacgatacga aaaaatagat aaaaaacatc attcttttac cacattattt
     5821 tccgtgaata tgaaaaataa tgttattact ttaatataag actatttttt gtttttccat
     5881 cactctgttc aaatttttcc gcatgacttg tgttttttgt aatacagctc gttttttaca
     5941 gctgaccaaa atcatcaatt aattatgcta aggaaataaa ttatgaaaac caaaaacttt
     6001 cttctttttt gtattgctac aaatatgatt tttatcccct cagcaaatgc tctgaaggca
     6061 gaaggttttc tcactcaaca aacttcacca gacagtttgt caatacttcc gccgcctccg
     6121 gcagagaatt cagtagtatt tcaggctgac aaagctcatt atgaattcgg ccgctcgctc
     6181 cgggatgcta atcgtgtacg tctcgctagc gaagatgcat actacgagaa ttttggtctt
     6241 gcattttcag atgcttatgg catggatatt tcaagggaaa ataccccaat cttatatcag
     6301 ttgttaacac aagtactaca ggatagccat gattacgccg tgcgtaacgc caaagaatat
     6361 tataaaagag ttcgtccatt cgttatttat aaagacgcaa cctgtacacc tgataaagat
     6421 gagaaaatgg ctatcactgg ctcttatccc tctggtcatg catcctttgg ttgggcagta
     6481 gcactgatac ttgcggagat taatcctcaa cgtaaagcgg aaatacttcg acgtggatat
     6541 gagtttggag aaagtcgggt catctgcggt gcgcattggc aaagcgatgt agaggctggg
     6601 cgtttaatgg gagcatcggt tgttgcagta cttcataata cacctgaatt taccaaaagc
     6661 cttagcgaag ccaaaaaaga gtttgaagaa ttaaatactc ctaccaatga actgacccca
     6721 taaagctgga cagcctgtat caggctatgg agggcccata gacaaatcta ccctatatga
     6781 gcataggagg agtctatggg cacaccacgt tttacccctg aatttaaggg attactggaa
     6841 aggctgggac atatccctcc ggcagaagca gaaaaagctt attatgctgc catcggaaac
     6901 gatgatctgg caacctgagt tcacagataa aacattctct aggaaactcg gggcggttcc
     6961 gttcaccaca tgcaatgtgg tgttgcaggg gaacggtctg caccatcccc tatgtcgatc
     7021 aatataacag aaatgacaac ttcagattca gggcacaacc taaatatatt ttaggtcacc
     7081 tctcaaatcg tttgcctgat accgctccat tttttaataa aaaaatcaat catttttgaa
     7141 tctttgcttt tcattgcatt gtcaagcata gtgtccccac tatttgcttt ttgaaatttt
     7201 ttgtttacat caaccaatcc attattgata aaatattcta gcactttata gttggcacca
     7261 tctttactaa gtaagtattc aacatcatac agattacaat atctattgtt tttcattttc
     7321 tccatctctg cgacatcttg ttttgtaaaa ttaaatttag atagtaaata aagtgctata
     7381 tcagcttttt tattgcctaa agaataagca agggcctgat gagccatttt tttatcagtc
     7441 actttattgt taatctcttc ataattagat gctttaatcg catcctccaa agatatgtct
     7501 ctgagtttta tttttttgaa gttatctgta cttagcattc ttggtatatg atactcaagc
     7561 tgaaacacaa agtttataat tctatccaga ctaacaggat caatattttt gttataacaa
     7621 aactctctga atccttgatc tttactcttt cttataaaat caataagatg caacccaatt
     7681 ccaagcctca tatctttagt gttaaatatt ggaacatcag ttttcccctg attaccatgc
     7741 acatacctgc taacttcatc cacaacctct ttaaagttat ctaagaaaaa tgattggtgc
     7801 tcatggtaaa aaacaggtgg aatagcatta tcaaagtgat cggttaatgt catatatcca
     7861 tatggagagt catgatcaat gatatacgca ttggcgccaa aatctacagt atgatgtgtt
     7921 ttatttagtg ggagtttttc ttgatgattg gaaatttcga ccccaaaaaa aacaaaatcg
     7981 tggttagaga tttctttaat atcctcagag tatgtattct tatgtgtttg tatattttca
     8041 gactgaattt tatcaaagga tttaatatta agaatattat ttgacagatt agagttactt
     8101 tgatgtctga agttaaaatt agaattaata ataatagaaa gtaagtgacg ttcgtctgaa
     8161 gaaagaggat gaactttttg tattatctca gagatatagt gcctggacac cttatcgaga
     8221 tgctcaagtg accgaaatga tgaccgtgct ccagatggcg cttcaagaga aagatttagt
     8281 gatttgaaac tggtcccttg agaaaacatt ttgctataac tttgcgctgc tatagacttc
     8341 cttaaaaagt ttttcacagt attagcacag tgatctaaat gtttttcact tatgccagaa
     8401 gaagatgagt ttgttacgcg taatacattt ttttgtaatg cttttgttgc tttattcgga
     8461 ttcgtttttc catcaacaag atcaatatta ttctgaacat taatatgatt tactgcttca
     8521 ggtattttca tccccccccc ccaattatgt gcgtttttat ctgtggttat ttagataaca
     8581 ttatctaact ggacactttc ttgtatgtat tatagctaaa tgaacagatt ttaatatgtt
     8641 gtatggtttt cactctgaaa tcccctgaat ttaatcacgt ttcctttact gaaggcaatt
     8701 ttaaccgtag tatcaattat taacccttgg gcttgatatc cctctcctct tctgggcacg
     8761 ggggatgtca gattacttat tacagaaacc aaaagaattg taagcgtcat ctcagtgtcg
     8821 tctatttcaa tgtctgatga ttggcgatga tagagtcctc ttataacgtt aatggactct
     8881 atcaatgtca gattctcttc gcccccgcag gattcgtgcc tcttactcta tgactctatg
     8941 gagtttaagc ttgatcttat tgaaaaatct taccagctag gggcctgcgt ggcccaactg
     9001 gccagagagt acgggat
//
LOCUS       DAEBXN010000153         8765 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.153, whole genome shotgun sequence.
ACCESSION   DAEBXN010000153 DAEBXN010000000
VERSION     DAEBXN010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8765
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.153"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            5..436
                     /locus_tag="KJL99_003803"
     CDS             5..436
                     /locus_tag="KJL99_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HBD4438371.1"
                     /translation="MALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKASKDIQFA
                     VQSGPMLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKA
                     KLNVEQLLYLGGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            complement(583..2745)
                     /gene="uvrD"
                     /locus_tag="KJL99_003804"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(583..2745)
                     /gene="uvrD"
                     /locus_tag="KJL99_003804"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HBD4438372.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAEI
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(2829..3545)
                     /gene="yigB"
                     /locus_tag="KJL99_003805"
     CDS             complement(2829..3545)
                     /gene="yigB"
                     /locus_tag="KJL99_003805"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HBD4438373.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(3545..4441)
                     /gene="xerC"
                     /locus_tag="KJL99_003806"
     CDS             complement(3545..4441)
                     /gene="xerC"
                     /locus_tag="KJL99_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HBD4438374.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(4438..5145)
                     /locus_tag="KJL99_003807"
     CDS             complement(4438..5145)
                     /locus_tag="KJL99_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HBD4438375.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(5142..5966)
                     /gene="dapF"
                     /locus_tag="KJL99_003808"
     CDS             complement(5142..5966)
                     /gene="dapF"
                     /locus_tag="KJL99_003808"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HBD4438376.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLDEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6003..6206)
                     /locus_tag="KJL99_003809"
     CDS             complement(6003..6206)
                     /locus_tag="KJL99_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBD4438377.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            6395..7873
                     /locus_tag="KJL99_003810"
                     /pseudo
     CDS             6395..7873
                     /locus_tag="KJL99_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659238.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            7870..>8765
                     /locus_tag="KJL99_003811"
     CDS             7870..>8765
                     /locus_tag="KJL99_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HBD4438378.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRAGLRLVKNISGKTGYIPSDEISIENEEYHWVTDGRVILWRGKIVS
                     NPPGTLTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVELATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSDVTVLEKRWWGINPRLMSVNRNSYSNDLLIRSGQNIYLNGVH
                     LTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESRVRWR
                     KQLTPDRQWSKWIDLPGIEPEQ"
BASE COUNT         2034 a         2388 c         2252 g         2091 t
ORIGIN      
        1 gaaggtggcg ttaaatctcg cttcaggtga agggaatttc tttatccgtc ctggcggcgt
       61 gttttatgtc gcgggagata aagtcggcat cgttcgtctg gatgctttta aagccagtaa
      121 agatattcag tttgcggtgc agtcaggacc aatgttgatg gaaaacggtg taattaatcc
      181 gcgtattcat cccaacgtcg cctcacgtaa aattcgtaac ggtgttggga ttaataaaca
      241 tgggaatgcc gtgtttttgt tgagccagca ggcgacaaat ttttatgatt ttgcctgtta
      301 tgccaaagcg aaactgaacg ttgagcagct gctttatctt ggtggcacga tttcacatat
      361 gtatatgaaa ggcggggcaa tcccgtggca acgctatcct tttgtcacca tgatttccgt
      421 cgagcggaaa ggttagaatg atagctttgt gtcagatact aaatatgctg ctgatgccgg
      481 atgcggcgta aacgccttat ccggcctaca tggcgttgca atttattgaa tctgaatgat
      541 tttttaggcc aaataaggtg cgcagcaccg catccggcaa cgttacaccg actccagccg
      601 ggcgtaagct gccaccagcc atttgattcc ctggccctgg aatgccacct gcaaacggct
      661 atgttcaccg ctgccttcca tattgacgat ggtgccttca ccaaacttag cgtggcgtac
      721 acgctggccg agcttgtagc cgctgtcgtt ctcgaccatc ggcgtaccca tccgctgatg
      781 gctgaccggg cggcttaccg tggcgcgcag gcgcacctct tccacacact cttccggcaa
      841 ctcgccaata aagcgcgacg ggcgatggta aacctctttg ccatacagac ggcgagtttc
      901 cgcgtaggtc agcgtcagtt tctgcatcgc gcgggttacg ccaacgtagg ccagacggcg
      961 ttcttcttcc agacgcccgc cttcatccag cgacatctgg cttgggaaca tgccctcttc
     1021 cataccgacg ataaacacct gcgggaactc cagacctttc gccgagtgca tggtcatcag
     1081 ttgtaccgca tcctgccagg tatccgcctg cccttcgcct gcttccagcg ccgcatggga
     1141 taggaacgcc tgcagcggca ttaaatcttc gtcttcttcg ttgtagctga actggcgcgt
     1201 tgccgtcacc agttcctcta agttttcgat acgcgtctga cctttttcgc ccttctcctg
     1261 ctcgtacatg gtacgcaggc cggagtcttt aattacccgg tcagtctgta catgcagcgg
     1321 catatcggca gtttcctgcg ctaaggcgtc gatcagttcc ataaaccgct gtaaggcgct
     1381 ggcagcacgt ccggcgaggg ctttttcctg caacagttca cgacatgcct gccagagtgt
     1441 taactggcga tcgcgcgatg tctgacgtac cacgtccagc gtccggtcac caataccccg
     1501 cgttggcgta ttcaccacac gctcaaaggc cgcgtcgtcg ttacggttgg caatcaggcg
     1561 cagatacgag agcgcatctt tgatttcctg gcgttcgaag aagcgcatcc cgccgtaaat
     1621 acggtacggc atactcgcct gcaacagtgc ttcttccagc acacgcgact gggcgttact
     1681 gcggtagaga atggcgcact cggcaagcgc cccgccgttg tcctgccagg ttttgatgcg
     1741 gttaaccaca aaacgcgctt catcgagttc gttaaaagcg caatagaggg aaataggctc
     1801 accgtccgcg ccatcggtcc acagtttttt acccagacgc ccgttattgt tttcaatcag
     1861 ggcgttagcg gcgctcagaa tattgctggt agagcggtag ttttgctcca gacgaataat
     1921 ttcggcaccg gggaaatcat taaggaaacg ctgaatattc tccacctgcg ccccgcgcca
     1981 gccgtagatt gactggtcgt catcaccgac gatcatcact ttgccggtgt cgcccgccag
     2041 caggcggatc cacgcgtact gaatgttgtt ggtatcctgg aattcgtcca ccaggatatt
     2101 ggtaaaacgt tcgcggtagt gttgtaggat atgcggcttg ttaagccaca gttcgtgagc
     2161 gcgcagcagc agctcggcga agtccaccag gcccgcgcga tcgcacgctt cctgatacgc
     2221 ctgatacacc ttctgccagg tctgctccac cggattaccg tagctttgaa tatgatgcgg
     2281 acgcaggcct tcatctttct ggctgttgat gtaccacatt gcctggcgcg gcggccactg
     2341 cttctcgtcg aggttcatgg ctttgatcag acgcttaagc aggcgtagct ggtcttcgct
     2401 atcgaggatc tggaaatcct gcggcagatt ggcgtccata tggtgcgcac gcagcaggcg
     2461 gtgcgccagc ccgtggaagg tgcctaccca catgccgccc tggctggtgc ccatcagttg
     2521 tccgatacga tgacgcatct ccgccgccgc tttgttggta aacgtcacag ccataatcga
     2581 gtatggcgag cagttttcca cgctcatcag ccaggcgata cgatgcacca gtacgcgcgt
     2641 cttaccactg cccgcgcccg ccagcaccag aaggttgctg cgtggcgcgg ccaccgcttc
     2701 gcgttgtttg tcattaaggc tgtcgagcag gtaagaaacg tccattggca ccgccgcgta
     2761 aaaataggtt gggcggagaa gcgcaacgcc ctccgccaaa aagctgggta tatatacaga
     2821 tttgctgatt atatcagcga ggtcagagat gccaaccggg aaatttccag atgtggcagt
     2881 aaacggctgt cccaggtttg catcagatcg ccattttccg gtctgatcca acaagcctgc
     2941 attccgctgc gaattgcccc acccacgtca gtggtgagat cgtccccaac atgtaagatc
     3001 tcgccgatcg gcacgttgag tttttccgca gccaaaaagt acatatcgct gaacggtttt
     3061 gagcgcccgt gcgggccagc gcgcagcaca aactcaaaat aatcccccaa accaaacagt
     3121 tccggctggg cgttaccgtt ggtgatcgcc accagcggcc atttcttcgc cagctgtttt
     3181 aaggtgtcgt gagtttgctg cgggacgtcg attcggctgc gccatttggc aaagttaatc
     3241 attgctgcgt gtgcgcctgc actggcttct tcggcactca gcccggcgtc gagcatcgct
     3301 tgttcaatcg aacgaaaacg ccaacgtgtc acgtcgtgat aaatctcggg ttccgcttcc
     3361 cgtaccgcct ggcgcaggcg ttgcagatct tcattctgga agctgcgcag cgccggatga
     3421 taattttgca caaaggtaag cgcctctcgc tcggtgcgca aaatcaccgg acggttatcg
     3481 taaagggtat catccaggtc aaaggtgagc gccgagatgc gccccaaagg ccggtaaaaa
     3541 cgcattattt cccccgtttg gcgcgtggat gcgccgcatc gtacaccgag gcaaggtgtt
     3601 gaaaatcaag atgagtatag atttgcgtgg tggagaggtt ggcatgaccc agtagctcct
     3661 gcacaccacg aagatcgccg ctcgactcca gcatatgcgt agcgaacgag tgacgtaatt
     3721 tatgtgggtg aacgtgatta ttcagccctt gttttatgcc ccattcggca aagcgtttct
     3781 gcacattacg cgcggagata cgcttgccca gtttcgacag aaaaagcgcg tcgtcttcgc
     3841 taccaaacag gtcgcgcaaa tcaagccagt gctcaatcca cgccacagcg ttgcgaccaa
     3901 tcggcaggcg gcgctctttg ctgcctttcc ccatcaccca cacttcgccg gactccaggt
     3961 cgaggtgttt gatatccagc cccaccagct cggaaagacg cagacccgcg ccgtacatca
     4021 cttccagcat tgcacggtcg cgtacagcga ggggatcgtt gatatcaata tccagcagcc
     4081 gattcatatc gtcgacgtcg atatttttcg gcagatgacg cggcgctttc ggtgccgaaa
     4141 ctcctttcgc cgggttagct ttgagttcgt tctggctgac cagccagtca aaaaagctac
     4201 gtagcgcaga aagccgtaac gccagacttg ctgctcccag ccctttacgg cgactgcgta
     4261 cagcaaaatt gcgtaccatc gctgcatcgc attgttgcca gctttgcagg ccgttttcgc
     4321 tggcaaaatt gatgatcgcc tcaagctgac gctggtagtt aagtagggtt atcgggctaa
     4381 gctgacgctc cacgctcaga taacgtaggt agcgttctac atcggtgtgt aaatcggtca
     4441 tacgcgttca atccaacgct ccagaagctc cggcaacatc agcgcaattt catgaagtaa
     4501 ctgcgttccc tgcccttgtt gatagtgact ggcatcgcga ctggtaaaca gcacaacacc
     4561 caaatcagca tcgcttccca gcatcgacat cgccaccgat cccaccgctt tcgcttccgg
     4621 tagcaccacc agcagctccg gtccgttaag cggcccaaga tagtgctgtt cctgccccaa
     4681 acgctgaata cgcagcggtt cgaaagactg acggcttaat gccagatgag tgtggttcga
     4741 cggcgcacct aagcgccagc gatctggaaa caggcgcaga ctcgcaccag ctaggccgag
     4801 atcgcgcgcc cagcggtgaa agcgcatcag catatcgtcg agactgctgg cggcggtgag
     4861 actgcgctgc aggtagagta ggcgataaaa cagcccttcg ttggcgatag cctgctccat
     4921 caacaacgcc atgttctctt ccagaacatg aatgtgatta cgtgcgcggg ccatgtgcca
     4981 ctcgaccaac gaaacggtgc cgcgtaccgg atgcggcaca cgtatcgctt ctactgcgcg
     5041 cgcattgcgg ataaaaaact caggattttt aatcagataa tcgacaaccg cccggtcatc
     5101 aagctccgtg agtgtttcct gcagttcttc ccctggttgc ttcatagatg aataaatcca
     5161 tcgtagacat gtaccgccgg gccagtcata tataacgggt gacccggacc tttccaggcg
     5221 atatcaagac gaccgccggg gagttccacg cgtacttctt cgtccagcaa accttgctga
     5281 atccctaccg ccaccgccgc acacgcgccg ctgccgcagg cctgggtttc tcctgcccca
     5341 cgctcataaa cgcgtaaacg aatatgctcg cgcttaacca cttgcataaa accgatattg
     5401 gcgcgctccg gaaaacgctc gtgactttcc agaacaggac caagcgtttc taccgccgcg
     5461 gtatcgacat catcgacctg aatcacgcaa tgcggatttc ccatcgacac cacgccgcat
     5521 aagattgtct gctcggcggc gcgcataata taggtctttt ccgctttgtt agcgcgaaac
     5581 ggcacggcgg aaggttcgaa gttgggttcg cccatattta cgcggaccag atcatcatcg
     5641 gtgacggtca gaaccatccg cccgttggcg gtgctgacgc ggatatcacg cttattggtc
     5701 agtcctttca gacgcacaaa acgggcaaag cagcgcgcac cgttaccgca ctgcgccact
     5761 tcactgccat cagcattgaa aatgcgatag tgaaaatcca gttcaggatc atacggcggc
     5821 tcaaccacca gcagctggtc aaaccctacc cccaggtgcc gatccgccag gcgacgaatc
     5881 agctccggtg aaaaaaagac attctgcgtt accgcgtcga cgaccataaa atcgttgcca
     5941 aggccatgca ttttcgagaa ctgcatcatt tactccaatc acgcgggtac agaaactgac
     6001 ttttagtaat tcacctggga tggaccatcg ccagtggcgc gatcgttttt atccggcacc
     6061 gtggattgcg tttgcgtctc taccggtttg gtcggcggcg gtgcgttttt atctgcaggc
     6121 gggaaataga gcggaccttt cagaccgcag cccgtcaggc tgaagagagt aagtaataca
     6181 gtgagtgcct taaacacgtt tttcattatg gattgcctgt aaagttcatc gctttctgct
     6241 ttctatcatc gcaggagaag gcgtaaaagc aagcgtttag cgactcttta tgaagtctga
     6301 aaagtgagga aatgctgcga gtataacgct tactcgtgag gatttatgtt ctgctggcag
     6361 tttcgtgatc aacacctcgc gccaggatgg ctttatgcac tatcgcaatg tggtttgttt
     6421 gttgtcttgt tcattgtttt tatcgtcggc atggggatgt cggctttccg cacctgaaca
     6481 taatatttac cagaaacagg gaaaaggcgt ggtgtacctg cgcccttata aagaaacaaa
     6541 tctttctcta cctgacgtga attacaagag tttgcgtcgg ttacccaatt tattaataga
     6601 cccgacaaca ctagacgaat gggataaaga accgcctctg actgatttga caaccgacta
     6661 cctctatgag ggcgcacagg cctggtatcc tcattattca tggcatagcg atggtcgcaa
     6721 tatcctctac gcgggtgaag ttgtgcaaaa ccctcccggc acacctcccg tcgatgtcgc
     6781 ctcgttcaag gcatggggtg attttgccgc agataaacac agtctctatt ttgaaggcaa
     6841 gcgcaccgat gacaatggtg gtggaaatag cttggatatc aaaacgctgc atcaggtgga
     6901 attccggcct ccctgggatc ctgacttact ggggctgata ttacgtgacg ctaatttctt
     6961 gtatatcaat gggcatcgcc tcgctgaccc cgagagtttc cgtgtgctgg cgcagaagtc
     7021 ctgggatcag cgaggaaagt tttccaccac gtttaacccc tgcattgccg taccatttgg
     7081 cccctgggat accctggctc gtacacggac caaaatcctg ctcaacggcg aacagcttga
     7141 tgccgacccg gacacctttt ccgtcgtgcg ctggatgccc ggctcactct tgacctggcg
     7201 tgataaaaac gggctgcagc gtaaagtcct cgacaaggaa aatctggcgt gggatgaaga
     7261 tttaacaaag cactgtctgg atttttctct gctggaaaag aaagtgttct ggcgtaaagg
     7321 acctgcttgt aaacaggaag aattacccgg actcgatccg gagcagtttc aacccatcag
     7381 tgatgctgtc gcccagcatc aggactcgct ttataccatc atcgaaacag agtctggtaa
     7441 ccgtaagctg gagatcgtga aacttgatga tcccaatctt attatcaaca aacgtttcaa
     7501 cgccgggaaa cgccacggct atttacttac gcgtgccgaa gggtagccat accattccgg
     7561 tttacacgtg tttgaatctg acggaccgct gatcttactg gataaccgtt ctccggatga
     7621 acgcgaagcc catcttaatg accatccctt tttgcgcaga tggtatgccc gcgataaccg
     7681 ctacatttac agctttgatg gcgcgcagct ctggcgatac cgcaccgctg atccgaaaca
     7741 agttcgctta atctggaagg aacaacattc gggatatgtc tatggcgtaa attacaaaac
     7801 gggatatctg gacggaaaaa ttactgatga cggcgaattt attcctgccc cgcgcaatga
     7861 ggcgacaaaa tgaagggtca aataatcaca gttgcactca tcctgttggg acacacattt
     7921 ctgacgcctg ctgtacaggc catagggttc gactattaca atgatcacgg tgttatgtct
     7981 tacggtaaag gttattgggg ggataagaaa atcattgccc agtttccgga gatgagcagg
     8041 gccggtttgc gccttgtgaa aaatatctct gggaaaacgg gttatatacc ttccgatgaa
     8101 ataagcatag aaaatgagga ataccactgg gtgacagacg gccgcgttat tctctggcgt
     8161 ggcaaaatcg ttagcaatcc accgggaaca ctcactgtcg atattgccag ctttcaggct
     8221 atgggccgct tcgcggtcga taaatatagc ctctattttg acggacagcg caccgaaagt
     8281 aatagcggcg cgtcacgtgt tgagctggcg acactaaaag ctatcgaagg taactctacc
     8341 acgctggtgg atagtaaaaa tctctacttg tccggtcgac gtcaggggag cagcagtgat
     8401 gttactgtat tagaaaaaag atggtggggt attaatccac gtcttatgag tgtaaacaga
     8461 aattcatatt ccaatgatct gcttatccgc agtgggcaga atatttattt aaatggcgtt
     8521 caccttacgg cgaatgcaga ctcatttgag ataattcgct ggatacctca ttcactgctg
     8581 gtttttcgcg acaataaggg tctgcatcgt tatccctttg gtcaattatc aggcaaagcg
     8641 atacccgtag atgatgacgt ctcttttgaa gtaggggaaa gtcgcgttcg ctggcgtaaa
     8701 cagctcacgc ccgaccgtca gtggagcaag tggatagacc taccaggtat tgaacctgaa
     8761 caatt
//
LOCUS       DAEBXN010000154         8666 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.154, whole genome shotgun sequence.
ACCESSION   DAEBXN010000154 DAEBXN010000000
VERSION     DAEBXN010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8666
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.154"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(329..592)
                     /gene="rpsT"
                     /locus_tag="KJL99_003812"
     CDS             complement(329..592)
                     /gene="rpsT"
                     /locus_tag="KJL99_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HBD4438379.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            695..913
                     /locus_tag="KJL99_003813"
     CDS             695..913
                     /locus_tag="KJL99_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HBD4438380.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICSMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            921..1862
                     /gene="ribF"
                     /locus_tag="KJL99_003814"
     CDS             921..1862
                     /gene="ribF"
                     /locus_tag="KJL99_003814"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HBD4438381.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            1905..4721
                     /gene="ileS"
                     /locus_tag="KJL99_003815"
     CDS             1905..4721
                     /gene="ileS"
                     /locus_tag="KJL99_003815"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HBD4438382.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWLPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4721..5215
                     /gene="lspA"
                     /locus_tag="KJL99_003816"
     CDS             4721..5215
                     /gene="lspA"
                     /locus_tag="KJL99_003816"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBD4438383.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVLLAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            5302..5751
                     /gene="fkpB"
                     /locus_tag="KJL99_003817"
     CDS             5302..5751
                     /gene="fkpB"
                     /locus_tag="KJL99_003817"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBD4438384.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5753..6703
                     /gene="ispH"
                     /locus_tag="KJL99_003818"
                     /gene_synonym="lytB"
     CDS             5753..6703
                     /gene="ispH"
                     /locus_tag="KJL99_003818"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HBD4438385.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKHAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6769..7683
                     /gene="rihC"
                     /locus_tag="KJL99_003819"
     CDS             6769..7683
                     /gene="rihC"
                     /locus_tag="KJL99_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HBD4438386.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            7850..>8666
                     /gene="dapB"
                     /locus_tag="KJL99_003820"
     CDS             7850..>8666
                     /gene="dapB"
                     /locus_tag="KJL99_003820"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBD4438387.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNN"
BASE COUNT         1980 a         2157 c         2460 g         2069 t
ORIGIN      
        1 ggtctaatga ggagatattt atgaatggtt aaagtatata ggaatttaat tcaaaaatag
       61 agtggtctta agtttaatac ctcaatgtgt atcacaatat ccatattctt tgtgggggag
      121 tctggagatt gagtagatat tcttgttcag aatgtatcag ccgatggttc tacgattctt
      181 aagccacgaa gagttcagat agtacaacgg catgtctctt ttgactatct ggcaaccggc
      241 agtgtgttct ctcacgcatc acaaaagcag caggcataaa aaaacccgct tgcgcgggct
      301 ttttcacaaa gcttcagcaa attggcgatt aagccagttt gttgatctgt gcagtcaggt
      361 tagccttatg acgtgcagct ttgtttttgt ggatcagacc tttagcagcc tgacggtcca
      421 cgatcggttg catttcgtta aatgctttct gtgcagcagc tttgtcgcca gcttcgatag
      481 ctgcgtatac tttcttgatg aaagtacgca tcatagagcg acggcttgcg ttgtgcttac
      541 gagccttttc agactgaatg gcgcgcttct tagctgattt gatattagcc aaggtccaac
      601 tcccaaatgt gttctatatg gacaattcaa aggccgagga atatgccctt ttagccttct
      661 tttgtcaatg gatttgtgca aataagcgcc gttaatgtgc cggcactcgt tacgtagtga
      721 tggcgcagga ttctaccagc ttgcggggtg tgaatacagc ttttccgcga taaaaattgc
      781 agcaggcggt cagtttcttc ccgtgatttg ctccatggca atgaaaagcc acttctttct
      841 gatttcggta ctcaatcgcc ggttaacctt gaccgctgta caaggtatac tcggacgatt
      901 ttcactgttt tgagccagac atgaagctga tacgcggcat acataatctc agccaggccc
      961 cgcaagaagg gtgtgtgctg actattggta atttcgacgg cgtgcatcgc ggtcatcgcg
     1021 cgctgttaca gggcttgcag gaagaagggc gcaagcgcaa cttaccggtg atggtgatgc
     1081 tttttgaacc tcaaccactg gaactgtttg ctaccgataa agccccggca agactgaccc
     1141 ggctgcggga aaaactgcgt taccttgcag agtgtggcgt tgattacgtg ctgtgcgtgc
     1201 gtttcgacag gcgttttgcg gcgttaaccg cgcaaaattt catcagcgat cttttggtga
     1261 agcatttgcg cgtaaaattt cttgccgtag gtgatgattt ccgctttggc gctggtcgtg
     1321 aaggcgattt cttgttatta cagaaagctg gcatggaata cggcttcgat atcaccagta
     1381 cgcaaacttt ttgcgaaggt ggtgtgcgta tcagcagcac cgccgtgcgt caggcgctgg
     1441 ctgatgacaa tctggctctg gcagaaagtt tactggggca cccgtttgcc atctccgggc
     1501 gtgtagtcca cggtgatgaa ttagggcgca ctataggttt cccgacggcg aatgtaccgc
     1561 tacgccgtca ggtttccccg gtgaaagggg tttatgcggt agaagtgctg ggcctcggtg
     1621 aaaagccgtt acccggcgtg gcaaacatcg gaacacgccc aacggttgcc ggtattcgcc
     1681 agcagctgga agtgcatttg ttagatgttg caatggacct ttacggtcgc catatacaag
     1741 tagtgctgcg taaaaaaata cgcaatgagc agcgatttgc gtcgctggac gaactgaaag
     1801 cgcagattgc gcgtgatgaa ttaaccgccc gcgaattttt tgggctaaca aaaccggctt
     1861 aagcctgtta tgtaatcaaa ccgaaacacg gaaccgagaa tctgatgagt gactataaat
     1921 caaccctgaa tttgccggaa acagggttcc cgatgcgtgg cgatctcgcc aagcgcgaac
     1981 cgggaatgct ggcgcgttgg actgatgatg atctgtacgg catcatccgt gcggctaaaa
     2041 aaggcaaaaa aaccttcatt ctgcatgatg gccctcctta tgcgaatggc agcattcata
     2101 ttggtcactc ggttaacaag attctgaaag acattatcgt gaagtccaaa gggctttccg
     2161 gttatgactc gccgtatgtg cctggctggg actgccacgg tctgccgatc gagctgaaag
     2221 tagagcaaga atacggtaag ccgggtgaga aattcaccgc cgccgagttc cgcgccaagt
     2281 gccgcgaata cgcggcgacc caggttgacg gtcaacgcaa agactttatc cgtctgggcg
     2341 tgctgggcga ctggtcgcac ccgtatctga ctatggactt caaaactgaa gccaacatca
     2401 tccgcgcgct gggcaaaatc atcggcaatg gtcacctgca caaaggcgcg aagccggtgc
     2461 actggtgcgt agactgccgt tctgcactgg cagaagcgga agttgagtat tacgacaaaa
     2521 cttctccgtc cattgacgtc gctttccagg cggtcgatca ggatgcgctg aaagcgaaat
     2581 ttggcgtaag caacgttaac ggcccaatct cgctggtgat ctggaccact acgccgtgga
     2641 ctctgcctgc gaaccgcgca atctctattg cacctgattt cgactatgcg ctggtgcaga
     2701 tcgacggtca ggccgtgatt ctggcgaaag atctggttga aagcgtaatg cagcgtatcg
     2761 gcgtgaccga ttacaccatt ctcggcacgg taaaaggtgc agagcttgag ctgctgcgct
     2821 ttacccatcc gtttatgggc ttcgacgttc cggcaatcct cggcgatcac gttaccctgg
     2881 atgccggtac cggtgccgtt cacaccgcgc ctggccacgg cccggacgac tatgtgatcg
     2941 gtcagaaata cggcctggaa accgctaacc cggttggccc ggacggcact tatctgccgg
     3001 gcacttatcc gacgctggat ggcgtgaacg tcttcaaagc gaacgacatc gtcgttgcgc
     3061 tgctgcagga aaaaggcgcg ctgctgcacg ttgagaaaat gcagcacagc tatccgtgct
     3121 gctggcgtca caaaacgccg atcatcttcc gcgcgacacc gcagtggttc gtcagcatgg
     3181 atcagaaagg tctgcgtgcg cagtcactga aagagatcaa aggcgtgcag tggctcccgg
     3241 actggggcca ggcgcgtatc gagtcgatgg ttgctaaccg tcctgactgg tgtatctccc
     3301 gtcagcgcac ctggggcgta ccgatgtcac tgttcgtgca caaagacacg gaagagctgc
     3361 atccgcgtac cctcgaactg atggaagaag tggctaaacg cgttgaagtt gacggcatcc
     3421 aggcgtggtg ggatctcgat gcgaaagaga tcctcggcga cgaagctgac cagtatgtga
     3481 aagtgccgga tactctcgac gtatggtttg actccggatc tacccactct tctgttgttg
     3541 acgtgcgtcc ggaatttgcc ggtcacgcag cggacatgta tctggaaggt tctgaccagc
     3601 accgtggttg gttcatgtct tccctaatga tctccaccgc gatgaaaggc aaagcgccgt
     3661 atcgtcaggt actgacccac ggctttaccg tggatggtca gggtcgcaag atgtctaaat
     3721 ccatcggcaa caccgtttcg ccgcaggatg tgatgaacaa actgggcgcg gatattctgc
     3781 gtctgtgggt ggcatcaacc gactacaccg gtgaaatggc cgtttctgac gagatcctga
     3841 aacgtgctgc cgatagctat cgtcgtatcc gtaacaccgc gcgcttcctg ctggcaaacc
     3901 tgaatggttt tgatccagca aaagatatgg tgaaaccgga agagatggtg gtactggatc
     3961 gctgggccgt aggttgtgcg aaagcggcac aggaagacat cctcaaggcg tacgaagcat
     4021 acgatttcca cgaagtggta cagcgtctga tgcgcttctg ctccgttgag atgggttcct
     4081 tctacctcga catcatcaaa gaccgtcagt acaccgccaa agcggatagc gtggcgcgtc
     4141 gtagctgcca gactgcgctg tatcacatcg ctgaagcgct ggtgcgctgg atggcaccaa
     4201 tcctctcctt caccgctgat gaagtgtggg gctatctgcc gggcgaacgt gaaaaatacg
     4261 tcttcaccgg tgagtggtac gaaggcctgt ttggtctggc agacagtgaa gcaatgaacg
     4321 atgcgttctg ggacgagctg ttgaaagtgc gtggcgaagt gaacaaagtc attgagcaag
     4381 cgcgtgccga caagaaagtg ggtggctcgc tggaagcggc agtaaccttg tatgcagaac
     4441 cggaactggc ggcgaaactg accgcgctgg gcgatgaatt acgatttgtc ctgttgacct
     4501 ccggcgctac cgttgcagac tataacgacg cacctgctga tgctcagcag agcgaagtac
     4561 tcaaagggct gaaagtcgcg ttgagtaaag ccgaaggtga gaagtgccca cgctgctggc
     4621 actacaccca ggatgtcggc aaggtggcgg aacacgcaga aatctgcggc cgctgtgtca
     4681 gcaacgtcgc cggtgacggt gaaaaacgta agtttgcctg atgagtcaat cgatctgttc
     4741 aacagggcta cgctggctgt ggctggtggt agtcgtgctg attatcgatc tgggcagcaa
     4801 atacctgatc ctccagaact ttgctctggg ggatacggtc ccgctgttcc cgtcgcttaa
     4861 tctgcattat gcgcgtaact atggcgcggc gtttagtttc cttgccgata gcggcggctg
     4921 gcagcgttgg ttctttgccg gtattgcgat tggtattagc gtgctcctgg cagtgatgat
     4981 gtatcgctcg aaggccacgc agaagctaaa caatatcgct tacgcgctga ttattggcgg
     5041 cgcgctgggc aacctgttcg accgcctgtg gcacggcttc gttgtcgata tgatcgactt
     5101 ctacgtcggc gactggcact tcgccacctt caaccttgcc gatactgcca tttgtgtcgg
     5161 tgcggcactg atcgtgctgg aagggttttt accttccaaa gcgaaaaaac aataataaat
     5221 cctgccggat gcggcctaca gattgctacg aaatcgtagg ccgcatccgg caaaaatcct
     5281 caaaaataag agcaaacctg catgtctgaa tctgtacaga gcaatagcgc cgtcctggtg
     5341 cacttcacgc taaaactcga cgatggcacc accgccgagt ctactcgcaa caacggtaaa
     5401 ccggcgctgt tccgcctggg cgatgcttct ctttctgaag ggctggagca acacctgctg
     5461 gggctgaaag tgggcgacaa aaccaccttc tcgctggagc cagatgcggc gtttggcgtg
     5521 ccgtcaccgg acctgattca gtacttctcc cgccgtgaat ttatggatgc aggcgagcca
     5581 gaaattggcg caatcatgct ttttaccgca atggatggca gtgagatgcc tggtgtgatc
     5641 cgcgaaatta acggcgactc cattaccgtt gatttcaacc atccgctggc cgggcagacc
     5701 gttcattttg atattgaagt gctggaaatc gatccggcac tggaggcgta acatgcagat
     5761 cctgttggcc aacccgcgtg gtttttgtgc cggggtagac cgcgctatca gcattgttga
     5821 aaacgcgctg gccatttacg gcgcaccgat atatgtccgt cacgaagtgg tgcataaccg
     5881 ctacgtggtc gatagcttgc gcgagcgtgg ggctatcttt attgagcaga ttagcgaagt
     5941 gccggacggc gcgatcctga ttttctccgc acacggtgtt tctcaggcgg ttcgtaacga
     6001 agcgaaaagc cgcgatttga cggtgtttga tgccacctgt ccgctggtga ccaaagtgca
     6061 tatggaagtt gcccgcgcca gtcgccgtgg cgaagaatct attctcatcg gccacgccgg
     6121 gcacccggaa gtggaaggga cgatggggca gtacagcaac cccgaagggg gaatgtatct
     6181 agtcgaatcg ccagacgatg tgtggaaact gacggtcaaa aacgaagaga agctctcctt
     6241 tatgacccaa accacgctgt cggtggatga cacgtctgat gtgatcgacg cgctgcgtaa
     6301 acgcttcccg aaaattgtcg gtccgcgcaa agatgacatc tgctacgcca cgactaaccg
     6361 tcaggaagcg gtacgcgccc tggcagaaca ggcggaagtt gtgttggtgg tcggttcgaa
     6421 aaactcctcc aactccaacc gtctggcgga gctggcccag cgtatgggca aacacgcgtt
     6481 tttgattgac gatgcgaaag atatccagga agagtgggtg aaagaggtta aatgcgtcgg
     6541 cgtgactgcg ggcgcatcgg ctccggatat tctggtgcag aatgtggtgg cacgtttgca
     6601 gcagttgggt ggtggtgaag ccattccgct ggaaggccgt gaagaaaata ttgttttcga
     6661 agtgccgaaa gagctgcgtg tcgatattcg tgaagtcgat taagtcatta gcagcctaag
     6721 ttatgcgaaa atgccggtct tgttaccggc attttttatg aagaaaacat gcgtttacct
     6781 atcttcctcg atactgaccc cggcattgac gatgccgtcg ccattgccgc cgcgattttt
     6841 gcccccgaac tcgacctgca actgatgacc accgtcgcgg gtaatgtctc ggttgagaaa
     6901 actacccgca atgccctgca actgctgcat ttctggaatg cggagattcc gctcgcccag
     6961 ggagccgctg tgccgctggt acgcgcaccg cgtgatgcag catctgtgca cggcgaatcg
     7021 ggaatggctg gctacgactt tgttgagcac aaccgaaagc cactcgggat accggcgttt
     7081 ctggcgattc gggatgccct gatgcgtgca ccagagcccg ttaccctggt ggccatcggc
     7141 ccgttaacca atattgcgct gttactttta caatgcccgg aatgtaaacc gtatattcgt
     7201 cgtctggtga tcatgggcgg ttctgctggt cgcggcaact gtacaccaaa tgccgagttt
     7261 aatatcgctg ccgatccaga agcggctgcc tgtgtcttcc gtagcggcat cgaaattgtc
     7321 atgtgcggtc tggatgtcac caatcaggca atattaaccc ctgactatct cgctacttta
     7381 ccggagttga atcgcaccgg aaaaatgctc cacgccctgt ttagccacta ccgtagcggc
     7441 agtatgcaaa gcggtctgcg aatgcacgat ctctgcgcca tcgcctggct ggtgcgcccg
     7501 gacttattca ccctcaagcc gtgttttgtc gcggtagaaa ctcaaggcga atttacctca
     7561 ggcacgacgg tggttgatat cgacggttgc ctgggcaaac cagccaatgt acaggtggca
     7621 ttggatctga atgtaaaagg cttccaacag tgggtggctg aggtgctggc tctggtgccg
     7681 taacctgtca catgttatcg gcatgcagtc attcatcgac tcatgccttt cactgatatc
     7741 cctccctgtt tatcattaat ttctaattat cagcgttttt ggctggcggc gtagcgatgc
     7801 gctggttact ctgaaaacgg tctatgcaaa ttaacaaaag agaatagcta tgcatgatgc
     7861 aaacatccgc gttgccatcg cgggagccgg ggggcgtatg ggccgccagt tgattcaggc
     7921 ggcactggca ttagaaggcg tgcagctggg cgcggcgctg gagcgcgaag gatcttcttt
     7981 actgggcagc gacgccggtg agctggctgg tgcagggaaa acaggcgtta ccgtgcaaag
     8041 cagtcttgat gcgataaaag atgattttga tgtgtttatc gattttaccc gtccggaagg
     8101 tacgctgaac catctcgctt tttgtcggca gcatggcaaa gggatggtga tcggcactac
     8161 ggggtttgac gaagccggta aacaggcaat tcgtgacgcc gccgccgata ttgcgattgt
     8221 ctttgccgcc aattttagcg ttggcgttaa cgtcatgctt aagctgctgg agaaagcagc
     8281 caaagtgatg ggtgactaca ccgatatcga aattattgaa gcacatcata gacataaagt
     8341 tgatgcgccg tcaggcaccg cactggcaat gggagaggcg atcgcccacg cccttgataa
     8401 agatctgaaa gattgcgcgg tctacagtcg tgaaggccat accggtgaac gtgtgccagg
     8461 taccattggt tttgctaccg tgcgtgcagg tgacatcgtt ggcgaacata ccgcgatgtt
     8521 tgccgatatt ggcgagcgtc tggagatcac ccataaggcg tccagccgca tgacatttgc
     8581 taacggtgcg gtaagatcgg ctttgtggtt gagtggtaag gaaagtggtc tttttgatat
     8641 gcgagatgtg cttgatctca ataatt
//
LOCUS       DAEBXN010000155         8496 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.155, whole genome shotgun sequence.
ACCESSION   DAEBXN010000155 DAEBXN010000000
VERSION     DAEBXN010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8496
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.155"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            45..398
                     /locus_tag="KJL99_003821"
     CDS             45..398
                     /locus_tag="KJL99_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HBD4438388.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(435..2513)
                     /gene="flhA"
                     /locus_tag="KJL99_003822"
     CDS             complement(435..2513)
                     /gene="flhA"
                     /locus_tag="KJL99_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HBD4438389.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAGGHIFGGCEFIRYDERPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     YERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGSGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGANAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNSVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(2587..3597)
                     /gene="hypE"
                     /locus_tag="KJL99_003823"
     CDS             complement(2587..3597)
                     /gene="hypE"
                     /locus_tag="KJL99_003823"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HBD4438390.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAGSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            complement(3594..4715)
                     /gene="hypD"
                     /locus_tag="KJL99_003824"
     CDS             complement(3594..4715)
                     /gene="hypD"
                     /locus_tag="KJL99_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HBD4438391.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            complement(4715..4987)
                     /gene="hypC"
                     /locus_tag="KJL99_003825"
     CDS             complement(4715..4987)
                     /gene="hypC"
                     /locus_tag="KJL99_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HBD4438392.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            complement(4978..5850)
                     /gene="hypB"
                     /locus_tag="KJL99_003826"
     CDS             complement(4978..5850)
                     /gene="hypB"
                     /locus_tag="KJL99_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HBD4438393.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPQMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHTPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            complement(5854..6204)
                     /gene="hypA"
                     /locus_tag="KJL99_003827"
     CDS             complement(5854..6204)
                     /gene="hypA"
                     /locus_tag="KJL99_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HBD4438394.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            6416..6877
                     /gene="hycA"
                     /locus_tag="KJL99_003828"
     CDS             6416..6877
                     /gene="hycA"
                     /locus_tag="KJL99_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HBD4438395.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            7002..7613
                     /gene="hycB"
                     /locus_tag="KJL99_003829"
     CDS             7002..7613
                     /gene="hycB"
                     /locus_tag="KJL99_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HBD4438396.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            7650..>8496
                     /locus_tag="KJL99_003830"
     CDS             7650..>8496
                     /locus_tag="KJL99_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005092744.1"
                     /note="catalyzes the oxidation of formate to carbon
                     dioxide and molecular hydrogen; formate hydrogenlyase
                     comprises of a formate dehydrogenase, unidentified
                     electron carriers and a hydrogenase (subunit 3); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HBD4438397.1"
                     /translation="MSPPLFWHFSFQKALSGWIAGIGGAVGSLYTAAAGFTVLTGAVG
                     VSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHAQVKCNGLQINMLMAA
                     AVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALRRLGTLLLVIACWLLWQR
                     YGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWVPQAHANASAPAAALF
                     STVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVGGLYALMEHNIQRLLAYH
                     TLENIG"
BASE COUNT         1904 a         2307 c         2288 g         1997 t
ORIGIN      
        1 gattaagatg taatataaat gcatcttaat gaggtggagg cagcatgtcc ggcaagcgta
       61 tctctcgtga aaaactgacg attaaaaaaa tgatcgatct ttatcaagcg aaatgcccgc
      121 aggcgtcagc ggagccggag cattacgagg cgttgtttgt ttacgcgcaa aagcggctgg
      181 ataaatgtgt gttcggcgaa gagaaaccag cctgtaagca gtgtccggtg cactgttacc
      241 agcctgcaaa gcgtgaagag atgaaacaga ttatgcgttg ggccgggccg agaatgctgt
      301 ggcgtcatcc gatcttaacc gtgcgtcatc tcatcgatga caagcgtccg gtgccagaac
      361 tgccggaaaa atatcgtccg aaaaaacctc gtgaatagta tgccggacag gcagatctgt
      421 ccggcaattt gcagttaaat caatgccgat ttatcaattc ccagccgctt catccgtgac
      481 agcagggtcg tgcgtttcag ccccagacgt tgcgcagcgc ctttaggccc ggcaaccacg
      541 ctgttggttt ctttcagcac gcgcacaatc aactgatatt catcttcgcc ctcctgggcg
      601 acaaccgttg cggcaggcgg cgtttcaggc tccggtaaag caatatctgg caatgacagc
      661 tgcagcacgt tgccgcgtgt tagcaatact gcgcgctcaa tgacgttttc cagttcgcgt
      721 acgttacccg gccactccat gttgctcaag gtgcgcagcg tctcggcagg aatgctgtcg
      781 atattgcgcc ccagacgacg ggcaattttg aaggtaaagg ctttcgccag cagcggaata
      841 tcttccggac gctcgcgtag tggcggcagg tgaatcggga atacgttcag gcggtaatag
      901 agatcgctac ggaactctcg gtcggcgacc atttttttca gatcgcggtt agtcgcggca
      961 attaaacgta cgtccgtctg aatgattttg ttgctgccga gacgttcaaa ctcctgttcc
     1021 tgcaatacac gcagcaactt cggctgtaac tccagtggca tatcgcccac ttcgtcgagg
     1081 aacagagagc ttttatccgc cagttcaaaa cgaccaatgc gctgggcgtt ggcaccggta
     1141 aaagccccac gctcatgacc aaacagatcg ctttccagca atccggcagg catcgccgcg
     1201 cagttcattt tgaccatgcg gcgattatta cgcccactga gattatggat cgcacgggca
     1261 atcagctctt tacccgagcc agtttcaccg aggatcagca cggtactgtc actttgcgcc
     1321 accatttcaa cttgtttaag cacgctgtac atggcttcgc tgcggccaat aatctcgcca
     1381 aattcactat caacattgtt gagctgctcg gtcagggcga ggttttcatc aaccagccgt
     1441 tctttcagac gatggatttc ctgataggcg agggcgttat cgacagcgat tgccacacgt
     1501 tcggcaatct ggcgcagcaa attcagattg gtagtggtaa acactttctc ttcgcattgc
     1561 gccagtttca gcacgcccag catggtgtcg ccagacatca gcggtaacag gcacaaggtt
     1621 tgaatctggt tgccccaggt gtcgaacaac atgcgttcat agggggctaa atcgtcccgc
     1681 tcgtagagat tgatcagcag catctcttta cttttgaaca cgcgttcggt gagggttccg
     1741 gcttcatcga cttcgctctg ttcgtgggcg ggatgttgtt tatcaagata gtgagtggag
     1801 tagatgttga gtttgttttt acggtggctg cgtaagacaa tgctgatatc gtcaatgtcg
     1861 aaatagtaat ggatttcttt ggcgacttcg ctgaccagtt cgtccatatc caggcgggaa
     1921 agcaccgcgt tggtaatggc aaccaggatg cggaagttat cgcgctcccg gcataacaac
     1981 tcatagtcga cattgttgac gacgcggctc tggatttgtt cggtgacgac agaaacgatc
     2041 tgcgtaaatg tttgcagacg attgaactct ttttcgctcc agggacgctc gtcataacga
     2101 ataaattcac agccaccaaa aatatgccct cccgccgcca gtggcatcag gcaatagtga
     2161 ccaaatttgg ggtatagccc acctgcggcc agctgcggcc aggtttcaca aaattcttcg
     2221 taactgcaat gcagcgtatc agggcgcgac aaaatgctgc gtaccggacc gtgtgccaga
     2281 acagtttcgt cttcatattt aatgggggtg tctttttcac gcgacgcgta ataagacgca
     2341 cgttgagtct gcgcttgcca caacacaata gccgcgttgt cggcgagcgc agaacgcttt
     2401 accagttgcg aaagagcctc acacagcgag gccagatcgg gctgctgcaa tagtgtccga
     2461 gtgatgtcga acaacccttg ctgtccgaga tcactcatcg gtgtatatga cattttgttc
     2521 ttccaggaaa gcaccgcaaa gcggtgcaaa aggtcattga ctaactatag gagatttaga
     2581 attttattag catatacgcg gaagcggttc ggcgtgtggt aaatcgaggg ttcgtttcac
     2641 gccatacaga ccggcaagac gaacaccttt acgttccacc acttcaccaa tcagcgccgc
     2701 gtctttcccc agtggatggg aatgtaacgc tgccagcact tgctctgccg cgttgcgttc
     2761 aacggcgatc accagtttgc cttcgttggc aaagttcagg gcgtccagtc ccagcaattc
     2821 gcaaacgccg cgcacggcag gtttaaccgg cagcgctgat tcagaaattt caataccaca
     2881 accgcaggct gccgcgaact catgaaccac cgcgtttaca ccaccacggg tggcatcacg
     2941 cagcgctttc acgccgggaa tgtcacgcag cgtctgaata agcggcgtca gcaccgcgca
     3001 gtcgctgacc agttcgccat ccagccccag ctgctcacgc aggttaagga tagtcgcccc
     3061 gtggtcgccg agtgtaccgc taaccagcaa tacatcgcct gcggttagcg tctgtgcgcc
     3121 ccagtgaata ttcgccggaa ttgcgcccat gccagcggtg ttgataaaca gtttatctgc
     3181 cgcgccgcgc tgcaccactt tagtatcgcc agtaacgatg gcaatgcctg ccgtgcgggc
     3241 ggtttctgcc atgctggtca ctacggcttt cagtgtctcc atcggcaatc cttcttcgag
     3301 gataaagcca caggagagat agcgcggaat agcgccactg cccgcaacgt cattcgcggt
     3361 gccgcaaatc gccagcttgc cgatattacc gccaggaaag aacagcgggt caatgacgta
     3421 actgtcggtg gagaacgcca gacggtcgcc ttccgctacc agctgcgcca gatcaagacg
     3481 tgcctgatct tcctgctctg ccagccacgg gttggcaaag gcttccataa acaggctgtt
     3541 gattaattgc tgcatcgcct ggccgccgct gccgtgggcg agttggatat tattcacgct
     3601 tcactctcct gctgacgata ctgataccac gcggcgcacg ctccttcgga ggagaccatc
     3661 agtgcaccaa atgcggtttg aggattacag gtgttaccaa acagcgggca ttgatgcggc
     3721 ttacatttgc ccgttaatac ctcaccacaa cgcgcgcgcg ggtcatcgca gacctgctgc
     3781 ggtgccgggc ggaaatgtgc ttcggcatcg aatcgttgat aatccggcgt caggtgcacg
     3841 ccagaagatt caatcacgcc taagccgcgc cattcgctgt cgccgttgac acagaacaca
     3901 tcggcaatcg cctgttgcgc cagtaggtta ccggcatccg gtaccacccg acgatactga
     3961 ttctctacct tgctgtgggc cgctattttc tgctccacca gcatgaccac gccttgcagt
     4021 agatcaaggg gttcgaaacc agcgaccacc agcggacgat gaaaatcgct ggcgataaaa
     4081 ttataggcat cagtgccgat aaccatacta acgtggcccg gcgcgaggaa cgcgtcgata
     4141 ccgttatccg gctgttccag caaactgcgc agcgtcggga taagcgtaat gtgctggcag
     4201 aagaagtaaa aattctgcac atcacgcgct ttcgcctgtt gcagagtgat ggcggtggtc
     4261 ggcatggtgg tttcaaaacc taagccgaag aacaccactt tgcgggttgg attctcctgc
     4321 gccagtttca acgcatccat cggcgagtaa acgatgcgca catcggcacc gcgtgctttt
     4381 gcctgcaaca gcgatccctg tttccctggt acgcgcatgg cgtcgccaaa ggtacagaag
     4441 atgacttccg gatggctggc aatctccacg caggtgtcga ttctacccat cggcagtacg
     4501 cacaccgggc aacccggacc gtggataaac tcaacgtttt caggcagtaa ctggtcgagg
     4561 ccgaatttaa aaatggcgtg ggtatgaccg ccacacactt ccataatccg cagagggcgt
     4621 tcggcggtgt aagagagatg tgaagcacgt tcgcgcagat gctcaattaa ctgcatcacc
     4681 tgttccggcg cgcgatattc atcaacaaaa cgcattattt ttcctcgcca tacaacagcg
     4741 cgccgacatc cggctcaacg tcaaacatgt tttgtaaggc gtcgagagtg tcgcgtgctt
     4801 cggcttcatt aattacgctc atggcaaagc caacgtgaac cagtacccac tggcccacgc
     4861 gcggctgacc gttttcatcg cagctgccga ctaacgttaa atcgacatcg cgctgaatgc
     4921 cgcagacgtc gactttcgcc tggttgccgt caatggtgcg gatctggccg ggaacgccta
     4981 tgcacatcgc tgtgtctcca gccagttcag ccactggtcc atcccttcgc cgctggtggc
     5041 ggaaataagg atgatttcaa tttctggatt gacttcgcgg gcgcaggcga tgcacttctc
     5101 aacgtcaaag ttgagatacg gcaacaggtc aactttgttg agcagcatca gcgaggcggc
     5161 ggcaaacata tgcggatatt tcagcggttt gtcttcacct tcggtaacgg aaagcaccgc
     5221 tactttgtgt ttttcaccga gatcgaagct ggccgggcat acgaggttgc caacgttttc
     5281 gataaacaga ataccgttat cgtccagtgg cagacgcggt gcggcgtcgg caatcatctg
     5341 tgcgtcaaga tggcagcctt taccggtgtt cacctgaatc gctggtgtac cggtagcgcg
     5401 aatgcgtgcg gcatcgttca cggtttgctg gtcgccttca ataaccgcgc acggaacgct
     5461 gtctttcagg cgcattaagg tttccgtcag cagggtggtt ttaccggaac cagggctgga
     5521 aaccaggttg agcaccagtt gcttgcgggc agcaaagcgc gcgcggttgc gttcagccag
     5581 acggttgttt ttgtccagca cgtcaatttc gacttccagc atccgacgct ggctcatgcc
     5641 cggtgtgtga gtgcccgctt cgccatgacc gtaatgcagg tcgccttctt cagtctggct
     5701 gggggtaaat tcaggcgctt taatgccggt gattttcatc tgcgggcgtg ccgccggggc
     5761 aaatggcgcg ctacgaaacg cggaatgagg gttatgttca tcaccctcga tatacaggtt
     5821 gccttcaccg caaccgcatg ttgtacacat cgctcactcc tggtctattt ctatccgccg
     5881 aatctgtaaa ccgtcgtctg ccacaatctg tagcatgtca ccatgacact gtggacagcg
     5941 gcggacgcgc tgggtcagta gcgtcacata ctgttggcat gtttcacacc agcattcggc
     6001 ctcttgttct tcgaggtgca gtttacaacc ttccgccacg ctgccgcggc aaaccagatc
     6061 aaaacaaaag gcaagagagc tggtttcgac acaagaaaat gcgccaattt tgagccagac
     6121 cccagttacg cgttttgcgc cgtgttttgc ggcctgctgt tcgatcaatt ccagtgcccg
     6181 ttggcagagg gttatttcgt gcatatcgcc tcccattaac tattgccagc tacaagcaat
     6241 aattgcgcca gtgttgatta tccctgcggt gaataatgtc gatgatgtcg aaatgacacg
     6301 tcgacacggc gacgaaattc atctttagct taaaaatctc tttaataaca ataaattaaa
     6361 agttggcaca aaaaatgctt aaagctggca tctctgttaa acgagtaacc tgacaatgac
     6421 tatttgggaa ataagcgaga aagccgatta catcgcacag cggcatcgtc gcctacagga
     6481 ccagtggcac atctactgca attcgctggt tcaggggatc acgttatcga aagcgcgcct
     6541 gcatcacgcc atgagctgcg cgccggacaa agaactctgt ttcgtccttt ttgaacattt
     6601 tcgtatttac gtcaccctgg cggatggctt taacagccac accatcgagt attacgtcga
     6661 aacaaaagat ggcgaagaca aacagcggat tgcgcaggcg caactgagca ttgacggcat
     6721 gattgatggc aaggtcaaca tccgcgatcg cgaacaggtt ctggaacact atctcgaaaa
     6781 aatcgctggc gtttacgaca gcttatacac cgccattgaa aacaatgtgc cggtgaattt
     6841 aagccaactg gtaaagggac aaagcccggc agcatgagct gaggctttgc ccgttttgca
     6901 ggcgttacgc ctgtttgggg atgggcgtgt cgatgagtgt cgaaaatgac atttcatcgg
     6961 catgttttcg tcaaaaatga caatcacctg aggaatgcct ggtgaatcgt tttgtaattg
     7021 ccgactccac gctctgtatc ggctgccaca cctgtgaggc cgcctgttca gagacgcatc
     7081 gccagcacgg cctgcaatca atgccgcgcc tgagagtgat gctcaatgaa aaagaatctg
     7141 cgccgcagct ctgtcaccac tgtgaagatg caccctgcgc ggtggtctgc ccggttaacg
     7201 ccatcacccg cgttgatggg gccgtgcagt tgaatgaaag tctgtgcgta agctgcaaac
     7261 tgtgcggcat cgcctgcccg tttggcgcaa ttgaattttc cggcagccgt ccgctggata
     7321 ttccggcaaa cgccaatacc ccgaaagcgc caccggcacc gcctgctccg gcgcgtgtca
     7381 gcacattgct tgactgggtg ccaggtattc gcgcgatcgc cgtcaaatgt gacctttgta
     7441 gctttgatga acaaggtccg gcctgtgtgc ggatgtgccc gactaaagcc ctgcatctgg
     7501 tggataacac cgatatcgcc cgcgtcagca aacgtaagcg tgagctgacc tttaacacgg
     7561 actttggcga tctcaccttg tttcagcagg ctcaaagtgg agaggctaaa tgagcgcaat
     7621 ttccctgatc aatagcggcg tggcatggtt tgtcgccgcc gctgttctgg catttctctt
     7681 ttcaaaaagc gctaagtggc tggatagccg gaattggcgg cgcggttggt agtctgtata
     7741 cggcagccgc gggcttcact gtactgactg gcgcggttgg cgtgagcggt gcgctgtcgc
     7801 tggtaagcta cgatgtgcaa atctccccgc ttaacgcgat ttggctgatt accctcggcc
     7861 tttgcggtct gtttgtcagc ctctataaca ttgactggca tcgccacgcg caggtgaagt
     7921 gcaacggctt gcagatcaat atgttgatgg ctgccgccgt ctgcgccgtc atcgccagca
     7981 acctcggcat gttcgtggta atggccgaag tcatggcctt gtgtgcggtg ttcctcacca
     8041 gcaacagcaa agagggcaaa ctgtggtttg cgctgaggcg tcttggcact ctgctgctgg
     8101 tgattgcttg ctggctgctg tggcagcgtt atggcacgct ggatctgcgc ctgctggata
     8161 tgcgtatgca acagctgccg ctcggttccg atatctggct gctcggtgtg attggctttg
     8221 gcctgctggc tgggattatt ccgctgcacg gctgggtgcc gcaggcacat gctaacgcct
     8281 ctgcgccagc tgccgcgttg ttttctacgg tagtcatgaa aattggcctg ctgggcatgt
     8341 taaccctgtc actgctgggc ggtaatgcac cgctgtggtg ggggatcgcg ctgctggtgc
     8401 tcggcatgat caccgcgttc gtcggtggtc tgtatgcgtt gatggaacac aacatccagc
     8461 gcctgctggc ttaccacacc ctggaaaata tcggca
//
LOCUS       DAEBXN010000156         8347 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.156, whole genome shotgun sequence.
ACCESSION   DAEBXN010000156 DAEBXN010000000
VERSION     DAEBXN010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8347
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.156"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(463..1659)
                     /gene="hemY"
                     /locus_tag="KJL99_003831"
     CDS             complement(463..1659)
                     /gene="hemY"
                     /locus_tag="KJL99_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HBD4438398.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1662..2885)
                     /gene="hemX"
                     /locus_tag="KJL99_003832"
     CDS             complement(1662..2885)
                     /gene="hemX"
                     /locus_tag="KJL99_003832"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HBD4438399.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKIATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTSAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PAPQGDTPAAAPQGE"
     gene            complement(2907..3647)
                     /gene="hemD"
                     /locus_tag="KJL99_003833"
     CDS             complement(2907..3647)
                     /gene="hemD"
                     /locus_tag="KJL99_003833"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HBD4438400.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3644..4585)
                     /gene="hemC"
                     /locus_tag="KJL99_003834"
     CDS             complement(3644..4585)
                     /gene="hemC"
                     /locus_tag="KJL99_003834"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HBD4438401.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLCALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGEAPA"
     gene            4972..7518
                     /gene="cyaA"
                     /locus_tag="KJL99_003835"
     CDS             4972..7518
                     /gene="cyaA"
                     /locus_tag="KJL99_003835"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HBD4438402.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPRMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNLPPANQDHDTPLLQQYFS"
     gene            complement(7558..7878)
                     /gene="cyaY"
                     /locus_tag="KJL99_003836"
     CDS             complement(7558..7878)
                     /gene="cyaY"
                     /locus_tag="KJL99_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HBD4438403.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDRDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(7926..>8347)
                     /locus_tag="KJL99_003837"
     CDS             complement(7926..>8347)
                     /locus_tag="KJL99_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HBD4438404.1"
                     /translation="FHLITGNIAQYKDRLYVTKLSTFGEDQLEIIPLDTPDLVIDRSF
                     NSGKQHAYFIRQLRSKSLQIIPVNGSLTKNDRFAYDDRNVYTWTDTEVRITPSPCPAK
                     TRVREENVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
BASE COUNT         1868 a         2184 c         2159 g         2136 t
ORIGIN      
        1 tgatgctgtt tttagtttta acggcaatta atatatgtgt tattaattga atgaatttta
       61 tcattcataa taagtatgtg taggatcaag ctcaggttaa atattcactc aggaagttat
      121 tactcaggaa gcaaagagga ttacagaatt atctcataac aagtgttaag ggatgttatt
      181 tcccgattct ctgtggcata ataaacgagt agatgctcat tccatctctt atgttcgcct
      241 tagtgcctca taaactccgg aatgacgcag agccgtttac ggtgcttatc gtccactgac
      301 agatgtcgct tatgcctcat cagacaccat ggacacaacg ttgagtgaag cacccacttg
      361 ttgtcataca gacctgtttt aacgcctgct ccgtaataag agcaggcgtt tttttatgca
      421 tcagaaaggt cccggaggtg ctttcctccg ggtgagaagg aactactgtg gcgggttatt
      481 ctgcaacgtt aacatcaaac cgtcgcgacg catcgctgca gcttcttccg gcttgtgcag
      541 tctgtccagc gcgtcggcaa gccatgcgta atcgtaggcg tccggacgtt gtttcagcgc
      601 cgcgcggaag gcgagcgatg cttcctgcca ttcaccgtgc ttcatcagtg actgacccaa
      661 tgtgctccac aacagcgggc gatcaccgac gtttttgatt tgctggcgta gcactttttc
      721 cagctgttcc gggttattgg ttttcagacg cgggatcggc agcagtaggc gatcgtcata
      781 ctggcgtttc aggccgtcga tgataatttg ctgggcagta tcatgatcgt cacattcaat
      841 aagatgttcc gccattgcca cctgcaacgc tacctgatga cgcgttttcc ggctttggtt
      901 tttccaccag ttacgcaaac cttcactacc gttatcggca cgcgcctgat ccatcaggcc
      961 aatccatgcc tgttgttcca gcattgcacg atgttcttca tcaccaacat gggctttcgc
     1021 cattgatggg atgatatcca gcagagaact ccatgcccct gtgcggatat acgcctgttc
     1081 cgccagacgt aatacttcag gatggcgtgg cgtaacttcc agcagcttat ccacgccgtg
     1141 gcgtgcagca tggttttcat tacgggccag ttgcagacgt acacgggtga tttctaccgg
     1201 aatggtgtca ttgccggcca gctccgctgc gcgttccaga tgttggttgg cgcgtgcttc
     1261 atcaccacgt tgttgcgcgg cttcggcagc cagtagatag ttcaccaccg gttgttcagc
     1321 gtgatcggca tttttcgcca tcagcttttc aacttgctga taatcacctt ccgccagttt
     1381 cagcagcgcc tgttcggtct gcttacgcgc acggcgacgc ttacgtccga caaaccatcc
     1441 acgggtgtgc gcgccagtgc ggaagatccg ccgcagtagc cactcaatgg caaacagcac
     1501 taccatcgcc agaatcaata tgatcgccag gcccgtgacg ctggtttcga tattgtagtt
     1561 gtcggtctgg atcagcacat aaccctgatg accggcaatc atcgggccaa ccactatccc
     1621 cgcaatcagc aaaacaaaga gtaataacac ttttagcatg gttattctcc ttgcggcgcg
     1681 gctgccggag tatcaccttg cggtgcaggt gcaggtgcag gtgcaggtgc aggtgcaggt
     1741 gcaggtgcag gtgcaggttt agcttccgtt gtccccgctg ccggttgtgc cagcaggtta
     1801 cgcacgcgag tctgcatcag tttttccagc atcgcctggc tttgcagggt ttccggaaga
     1861 tccatcgaga tattttgctg gcttaactgg tccacctcgt cgaggaacgc tttggtggtg
     1921 gcatcatcag tatcgtagta agcacgtacc caggtggaga cgttctccag cgcctggcga
     1981 taagtctctt cctgatgacg cggtacagct tgtgctgcga ccagcaggcg agagcgaata
     2041 ttttcgcgca gatagatatc ctgatttggc gctaacagcg gtacggcggt gtcatcacga
     2101 cggcgaatcg taatgaagtt gtccataaag ttctgccagc ttttttgcag attgatacgc
     2161 cattcgctga tggaactgga aagctcttca ccgtctgaat ccatcggcga accatcgctg
     2221 tcattatcgg ccagacgcag gttatctacc tgatttgaaa gctgattaag cttaaggatg
     2281 atgccgtcat aatccacctg cgatactgca gaaaggctgg cgatatcatc ggtaattgcc
     2341 cgacgaacgg taatcagact cgggtcattc atatccgcca ggctggcgtc tgcacttttc
     2401 agcaacgctg cagaggtcgt gacgtcctga tcgctccaca gcttccgtcc ggcgagtttc
     2461 accagaaaat cggcctgagc cagcagccag gttttagcat cgctgccgga aatggtggcg
     2521 atcttttgtt ggacttcatc caactgtttt gccagcgttt cttgctgacg attcgcctgc
     2581 ttaagctgcg cagcttgttg cttaataatg ccttccagct cggctttttg gctctcctgg
     2641 gctttttgca atgccgtcag ttggttagcc agggcatcgc tggtggcggt ctgattgacg
     2701 gcctgttgtt taccccagcc atacaaaccg acacccgccg ccagagcaat agcgatagcc
     2761 accgcgctga gaatcaatgc agtattgttc ttactctttt tttctgttgc gacaggttgt
     2821 gacgtggtgt ccacggcctc cctggtctct tcaaccacgg cggaggtttt ttcttgttcc
     2881 gtcattatgg cttcctgtta tgagagttat tgtaatgccc gtaaaagcgc atcgttgtca
     2941 gcgttatcgg cgaccttaat gtcttgccag cccagttccc gggcgagttt cgccaaacgc
     3001 tcactgacga ccaatagtcg acagtgtagt aaccagtgct cacgatacca ttgtgggatc
     3061 agcgaccaga gttgctgcaa catttcaccg ctggtaacaa cgaccatcgt cacctcgcgg
     3121 gattgccagc gcatcgcttc ttctgcacca tcgtaatgga ttgcgcatcg ttgataacat
     3181 tcacaaaaag tgacctcagc accgcgcacc gtcagggtat ccccaattag ctcacgaccg
     3241 ccattgccac gtaatatcag cgcacgtttg cccgcaatat tttgtaattc aggtaattgt
     3301 agcaagactt cgctgatttc ccgatcctgt gggtagagaa tcttctgtcc gcttacggta
     3361 tgtagcgcca gtgcggtggt gcgtccaatg gcgaaataag taggtagttg gggccattta
     3421 cgatcttgct gatgcagctg tgattgggca aaagcaaccg cgtgttgcga gagggcaaac
     3481 aacagatcgc tctcccccag cgctgccagt tgatcagcaa gttgcggtaa ttgtcgaccc
     3541 ggagaaaact caatcagcgg aaaatgccag gccacctgcc ccagtgtgcg cagacggctc
     3601 actaactctt ctccagcggg agacgggcgg gtgaccagga tactcatgcc ggggcctctc
     3661 cgttatagac ttcagcgagg atctcgcgcg cgccgttatt cagtagctct tctgccagcg
     3721 aaatccccat ttgttcggca tcttgcggcg caccgcggcg ttcaccgcga ataatctgcg
     3781 aaccgtccgg cgcgccgacc agcgcacata gccagatttc gccatcaata agctcggcgt
     3841 agctaccaat tggcacctga catccgcctt cgagacgggt attcatggcg cgttctgcgg
     3901 taacgcgcag tgcagtttcg tggtgattca gcgcggcaag cagctcgcga gtgcgtgtat
     3961 catcaaggcg gcattcaata ccgactgcac cttgtcctac cgccggaaga gaaatctcgg
     4021 gtggtaacgc ggcgcgaatg cgggactcca gacctaaacg ttttagtccg gctacggcaa
     4081 gaatgatggc atcgtattcg ccgttatcta gtttgctcag gcgagtgccg acgttgccgc
     4141 gcagggagcg gataatcaga tccgggcggc gttcagccag ttggcactgg cgacgtaaac
     4201 tggacgtccc gacgatactg cctgctggta acgcatccag actgtcatag ttattggaca
     4261 caaaggcatc gcgaggatct tcacgctcgc aaatagtgac cagccccaga ccttgcggga
     4321 attcaaccgg cacatctttc attgaatgta cggcgatatc agcgcgattc tccaggagcg
     4381 cgacttccag ctcttttaca aataagcctt ttccgcctac tttcgccagc ggcgtatcaa
     4441 gaatcacatc gccgcgcgtc accatcggta ccagttcaac gaccaggccc ggatggctcg
     4501 ccatcaactt gtctttgaca tagtgtgcct gccagagtgc aagtgggctt tggcgtgtgg
     4561 caattcttaa aacattgtct aacatgcttg ttaccgtcat tatcatccgt ggtccatcct
     4621 aacatccttg ccagagtgat gtcagtgttg tggtgaaacg tagacgcctg cgcaaaccgt
     4681 aaaatgaggt ctggcagtgg atcctgacag gcgtttcacg ccgtcgtaat aaggaattta
     4741 cagagaataa acggtgctac acttgtatgt agcgcatctt tctttacggt caatcagcaa
     4801 ggtgttaaat tgatcacgtt ttagaccatt ttttcgtcgt gaaactaaaa aaaccaggcg
     4861 cgaaaagtgg taacggttac ctttgacata cgaaatatcc cgaatgccgc gtgttaccgt
     4921 tgatgttggc ggaatcacag tcatgacggg tagcaaatca ggcgatacgt cttgtacctc
     4981 tatattgaga ctctgaaaca gagactggat gccataaatc aattgcgtgt ggatcgcgcg
     5041 cttgctgcta tggggcctgc attccaacag gtctacagtc tactgccgac attgttgcac
     5101 tatcaccatc cgcgaatgcc gggttacctt gatggtaacg ttcccaaagg catttgcctt
     5161 tacacgcctg atgaaactca acgccactac ctgaacgagc ttgaactgta tcgtggaatg
     5221 tcagtacagg atccaccgaa aggtgagctt ccaattactg gtgtatacac catgggcagc
     5281 acctcgtccg tagggcaaag ttgttcctct gacctggata tctgggtctg tcatcaatcc
     5341 tggctcgata gcgaagagcg ccagttacta caacgtaaat gcagcctgct ggaaagctgg
     5401 gccgcctcgc tgggtgtgga agtcagcttc ttcctgattg atgaaaaccg cttccgtcat
     5461 aatgaaagcg gcagcctggg gggcgaagat tgtggctcca cccagcatat actgctgctt
     5521 gacgaatttt atcgtactgc cgtgcgtctc gccggtaagc gtattctgtg gaatatggtg
     5581 ccgtgcgacg aagaagagca ttacgacgac tatgtgatga cgctttacgc gcagggcgtg
     5641 ctgacgccaa atgaatggct ggatctcggt ggcttaagct cgctttctgc tgaagagtac
     5701 tttggtgcca gcctttggca gctctacaag agtatcgatt ccccatacaa agcggtgctg
     5761 aaaacactgc tgctggaagc ctattcctgg gaatacccga acccacgtct gctggcgaaa
     5821 gatatcaaac agcgtttgca cgacggcgag attgtatcgt ttggcctcga tccatactgc
     5881 atgatgctgg agcgtgttac tgaatacctg acggcgattg aagatttcac ccgtctggat
     5941 ttagtacgtc gctgcttcta tttaaaagtg tgcgaaaaac tcagccgtga acgcgcctgt
     6001 gtaggctggc gtcgcgcagt gttgagccag ttagtgagcg agtggggttg ggacgaagct
     6061 cgtctggcaa tgctcgataa ccgtgctaac tggaagattg atcaggtgcg tgaggcgcac
     6121 aacgagctgc tcgacgcgat gatgcagagc taccgtaatc tgatccgctt tgcgcgtcgc
     6181 aataacctta gcgtctccgc cagtccgcag gatatcggcg tactgacgcg taaactgtat
     6241 gccgcgtttg aagcattgcc aggtaaagtg acgctggtaa acccgcagat ttcacccgat
     6301 ctctcggaac cgaatctgac ctttatttat gtgccgccgg gccgggctaa ccgttcaggt
     6361 tggtatctgt ataaccgcgc gccaaatatt gagtcgatca tcagccatca gccgctggaa
     6421 tataaccgtt acctgaataa actggtggcg tgggcatggt ttaacggcct gctgacctcg
     6481 cgcacccgtt tgtatattaa aggtaacggc attgtcgatt tgcctaagtt gcaggagatg
     6541 gtcgccgacg tgtcgcacca tttcccgctg cgcttacctg caccgacacc gaaggcgctc
     6601 tacagcccgt gtgagattcg ccatctggcg attatcgtta acctggaata tgacccgaca
     6661 gctgcgttcc gcaatcaggt ggtgcatttt gatttccgta agctggatgt cttcagcttt
     6721 ggcgagaatc aaaattgcct ggtaggtagc gttgacctgc tgtaccgcaa ctcgtggaac
     6781 gaagtgcgta cgctgcactt caacggcgag caatcgatga tcgaagccct gaaaactatt
     6841 ctcggcaaaa tgcatcagga cgccgcaccg ccagatagcg tggaagtctt ctgttatagc
     6901 cagcatctgc gcggcttaat ccgtactcgc gtgcagcaac tggtttctga gtgtattgaa
     6961 ctgcgccttt ccagcacccg ccaggaaacc gggcgtttca aggcgctgcg cgtttctggt
     7021 caaacctggg gcttgttctt cgaacgcctg aatgtatcgg tacagaagct ggagaacgcc
     7081 atcgagtttt atggcgctat ttcgcataac aaactgcacg gcttgtcagt gcaggttgaa
     7141 accaatcacg tcaaattacc ggcggtggtg gacggctttg ccagcgaagg gatcatccag
     7201 ttctttttcg aagaaacgca agacgagaat ggctttaata tctacattct cgacgaaagc
     7261 aaccgggttg aggtgtatca ccactgcgaa ggcagcaaag aggagctggt acgtgacgtc
     7321 agtcgcttct actcgtcatc gcacgaccgc ttcacctacg gctcaagctt catcaacttc
     7381 aacctgccgc agttctatca gattgtgaag gttgatggtc gcgaacaggt gattccgttc
     7441 cgaacaaaat cgattggcaa cctgccgccc gccaatcagg atcacgatac gccgctatta
     7501 cagcagtatt tttcgtgatg agcgtgccgg aaagcgaggc ttatccggca tgcaatctta
     7561 gcggaaactg actgtttcac ccgcctgttg cgtcgccgcc tgttccagca aatcccagaa
     7621 ggtttcgccg ctgcgatcgc aaatccattc atcgcctttc aggtcaaaat ggtagccgcc
     7681 ctgtttggtt gccagccaca cctggtgcag cggctcctgg cggttaataa tgattttgct
     7741 gccgttttca aaggtaatgg tcagtacgcc gccgttgatt tcgcagtcga tatcgctgtc
     7801 gccatccctg tcgtccagac gctcttcaat ggtcagccac agttgatcag ccaggcgatg
     7861 aaattcactg tcgttcattg ttgtatcctg tttttaagtg atggcggcag tatagcggca
     7921 tggggtcagg gcttcaaagt ttgcacctct gctgctgcgt tccggcatga ttcatccgtc
     7981 accggaataa tgatgtctct gttttgaact tcacgtacgt tctcctctct gacacgagtt
     8041 ttcgccgggc agggggaggg cgtaatcctt acctctgtat cggtccatgt ataaacattg
     8101 cgatcgtcat aagcgaagcg atcgttttta gttagcgaac cgttaactgg aataatttgc
     8161 aagctctttg accgtaattg gcggataaag taagcatgct gtttgccgct attaaatgag
     8221 cgatcaatga ccaggtctgg cgtatccagc gggattatct caagctggtc ttcaccaaat
     8281 gtcgataatt ttgttacata cagccgatct ttatattgcg caatattgcc agtaatcaga
     8341 tggaatt
//
LOCUS       DAEBXN010000157         8309 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.157, whole genome shotgun sequence.
ACCESSION   DAEBXN010000157 DAEBXN010000000
VERSION     DAEBXN010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8309
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.157"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            94..1590
                     /gene="ravA"
                     /locus_tag="KJL99_003838"
     CDS             94..1590
                     /gene="ravA"
                     /locus_tag="KJL99_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HBD4438405.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFILRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTLIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEDSLQDVGAQIRQAQQC"
     gene            1584..3035
                     /gene="viaA"
                     /locus_tag="KJL99_003839"
     CDS             1584..3035
                     /gene="viaA"
                     /locus_tag="KJL99_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HBD4438406.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQFIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVFERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="KJL99_003840"
     CDS             complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="KJL99_003840"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HBD4438407.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQLSTLKSTVEAIWAGIKATEAAVNEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTLSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            4184..4642
                     /gene="asnC"
                     /locus_tag="KJL99_003841"
     CDS             4184..4642
                     /gene="asnC"
                     /locus_tag="KJL99_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HBD4438408.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4732..5175
                     /gene="mioC"
                     /locus_tag="KJL99_003842"
     CDS             4732..5175
                     /gene="mioC"
                     /locus_tag="KJL99_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HBD4438409.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEVGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGTIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            5554..7443
                     /gene="mnmG"
                     /locus_tag="KJL99_003843"
     CDS             5554..7443
                     /gene="mnmG"
                     /locus_tag="KJL99_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HBD4438410.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     RYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASLHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQISGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            7507..8130
                     /gene="rsmG"
                     /locus_tag="KJL99_003844"
     CDS             7507..8130
                     /gene="rsmG"
                     /locus_tag="KJL99_003844"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HBD4438411.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
BASE COUNT         2104 a         2116 c         2100 g         1989 t
ORIGIN      
        1 attacccccc tttgagacta ttttagtcac cagcaagaaa cgtctatact cgcaatttac
       61 gcagaacttt tgacgaaagg acgccacttc attatggctc accctcattt attagcggaa
      121 agaatttccc gcctgagcag ttcgttggaa aaggggcttt atgaacgtag ccacgccatc
      181 cgcttgtgtt tattagcggc attaagtggt gaaagtgtgt tcctccttgg cccgccaggt
      241 attgccaaaa gtttgatcgc ccggcgctta aaattcgcct ttcagaatgc ccgcgcgttt
      301 gaatatctga tgacccgctt ctccacgccg gaagaagttt ttggtcccct ttctattcag
      361 gcgctaaaag atgaagggcg ctatgaacgt ttaaccagcg gttacctgcc ggaagcagaa
      421 atcgtctttc tggatgagat ctggaaagcg ggcccggcaa ttcttaatac cttgctcacc
      481 gccattaacg agcgccagtt ccgcaacggt gcacacgtag aaaaaatccc gatgcgtcta
      541 ctggtggcgg cctccaacga gctgccggaa gcagacagca gtctggaagc gttatatgac
      601 cgcatgctga ttcgtctatg gttagataaa gtgcaagata aagcgaattt ccgctccatg
      661 ctgaccagtc aacaggatga aaacgacaat ccggttcctg atgccttgca ggtcacagat
      721 gaagaatatg aacgctggca gaaagagatt ggtgaaatta cgctgcccga tcatgtattt
      781 gagctgattt ttattctgcg ccagcaactg gataaattac cggatgcgcc ttatgtctcg
      841 gatcgtcgct ggaaaaaagc gatccgctta ttgcaggcca gcgccttttt tagcggtcgc
      901 agtgctgttg ccccggttga tctcattttg ctgaaagatt gcctgtggta tgacgcgcaa
      961 agcctgaatt tgatacaaca acaaattgat gtattgatga ccggtcatgc ctggcaacag
     1021 caagggatgt tgacccgcct gggcgcgatt gtgcaacgtc acctgcaact ccagcagcaa
     1081 caaagcgata aaacagcctt aacgttaatt cgtctgggcg gcattttcag ccgtcgtcag
     1141 cagtatcaac ttcctgttaa cgttactgct tccactctga ctctgctgct gcaaaaaccg
     1201 ttaaaactgc atgatatgga agtggttcat atctcctttg agcgtagcgc gctggaacag
     1261 tggctgagta aaggtggtga aattcgcggc aaactgaacg gtatcggctt tgcccagaaa
     1321 ctgaatctgg aagttgatag cacccaacat cttgttgtac gcgatgtaag tttacaaggc
     1381 agtacgctgg cacttcccgg ttcatcggct gaaggtctgc caggtgaaat aaaacaacaa
     1441 ctggaagagc ttgaaagcga ctggcgtaag caacacgctt tattcagcga acagcaaaaa
     1501 tgtctgttta tccctggcga ctggttaggt cgcattgaag acagcctaca ggatgtcggt
     1561 gcacagattc gtcaggcaca acaatgctaa cgctggatac gcttaatgtg atgctggccg
     1621 tcagcgaaga gggattgatc gaagagatga tcatcgcact gctggcctca ccgcagctgg
     1681 cggtcttctt tgaaaaattc ccacgactga aggcggcaat cactgatgat gttccccgct
     1741 ggcgtgaggc gctgcgcagt cggctgaaag atgcccgagt cccgccggaa ctcaccgaag
     1801 aggtgatgtg ctatcagcaa agccagctcc tctccacgcc gcagtttatt gtgcagctac
     1861 cacagatcct ggacttactg catcgtctga attccccatg ggcagaacaa gcccggcagt
     1921 tggttgatgc taacagcaca ataacttcag cgttacacac gctttttctc cagcgttggc
     1981 gtttaagtct gatcgtgcaa gcaacgacgt taaatcaaca gctattagaa gaagaacgcg
     2041 aacaactgtt aagtgaagtt caggaacgca tgacgctgag cggacaactt gaaccgattc
     2101 tcgcagataa caataccgca gctggtcgtc tgtgggatat gagcgccggt caacttaaac
     2161 gtggcgacta tcagtttatt gtgaaatacg gtgaatttct taacgaacag ccggaactga
     2221 aacgcctggc agagcagctg gggcgttctc gggaagccaa atcaataccg cgcaacgatg
     2281 cgcagatgga aaccttccgc accatggtgc gcgaaccggc gacggttcct gagcaggttg
     2341 atggtctgca acaaagcgat gatattttac gtctcctgcc gccagaactg gcgacactag
     2401 ggataacgga actggagtat gagttttacc gtcggctggt ggaaaaacag ttgctcacct
     2461 atcgcctgca cggtgagtcg tggcgtgaaa aagtgttcga acgtccggtg gtacataaag
     2521 attacgatga acagccgcgc gggccgttta ttgtctgtgt ggatacttcc ggctcaatgg
     2581 gcggctttaa tgaacagtgt gcgaaagcgt tctgcctggc tttgatgcgc attgctctcg
     2641 ctgaaaaccg gcgctgttat attatgctat tttccaccga gatcgtccgt tatgagcttt
     2701 caggcccaca aggcatcgaa caagcaatcc gttttttaag ccagcagttt cgtggcggca
     2761 ccgatcttgc cagttgtttt cgcgccatta tggaacgctt gcaaagcagg gaatggtttg
     2821 atgccgatgc ggtggtgatt tctgatttta tcgctcagcg gttgcctgac gacgtgacga
     2881 gtaaagtgaa agagctgcag cgggtacatc agcatcgctt tcatgccgtg gcgatgtcgg
     2941 cacacggcaa acccggcatc atgcgcattt tcgatcatat ctggcgcttt gataccggga
     3001 tgcgaagccg cctgctcaga cgctggcggc gataaattat tacagcagag aagggacgct
     3061 ctcgcgaaca gcagctggcc atactccaca ctgaacctgg ccgatatgcg gcagttgcag
     3121 cagcagcata gtcaaacgag actggccgat accgccgccg atggtctgcg gcatttcacc
     3181 gcgcagcagc gcctgatgcc attccagctg cagacgatct tcatcaccgg tcagcgccag
     3241 ctgatgcttc agcgtgtcgg catccacgcg gatccccatg gaagaaagct caaacgcatc
     3301 ttccagtacc gggttccaca ccagaatatc gccgttcagg cccgcatggc ccagctctga
     3361 cagggtgctc cagtcatcat aatccggtgc gcgcacgtcg tgacgatgac catcactcaa
     3421 cttgccgcca atcccgacga ggaataccgc gccaagatct ttcgcaatcg cccgctcacg
     3481 ccctttggca tcgagatccg gataacgaga cagtaactcc tggctgtgta cgaagtgaat
     3541 ctgatccggc aggaacggtg ccaggccaaa ctcttcgtta accgcagctt cggttgcttt
     3601 aattcccgcc cagatcgcct ctaccgtgct tttcagagtc gagagttgac gctcaccgtc
     3661 gcccattacg cgttcccagt cccactggtc aacatagacc gagtgcaacg gagaaagacg
     3721 gtcttcatcg gggcgaaggg ctttcatgtg cgtgtacagc ccttcgcccg cgctgaagtc
     3781 gtgttgccct aaggtctgac gtttccactt cgccagtgaa tgaaccactt cgaactgggc
     3841 atcaggcaga gctttcactt ttacctgcac cgctttttca cagcccgaca agttatcctg
     3901 cgtgccatcc cccacacggc taagaatcgg cgcctggact tcgatcagcc ccagacgttc
     3961 ttccagttgg cgagaaaagt gagatttcac gaagctaatt tggcgttgtt tggcaatgta
     4021 agcggttttc attttttata ctcctgcgtc ctgttgctta tgattaagca acaaaaaccg
     4081 acacacatgc aataatcatt caataaaaag cctaacacac ttttgattca ttaaaaaaga
     4141 cggctaaaat agaatgaata atcaatctgc ataagaaaat cctatggaaa attatctgat
     4201 cgacaatctg gaccgtggca tcctggaagc attaatgggc aatgcgcgca ccgcttacgc
     4261 cgaactggcg aaacaatttg gcgtcagtcc ggggacgatt cacgttcgag tagagaaaat
     4321 gaagcaggcg gggatcatta ccggggcgcg tattgatgtc agcccgaagc agctcggtta
     4381 tgacgtaggc tgctttatcg gcattatatt aaagagcgcc aaagactacc cttccgcgct
     4441 ggcaaagctg gaaagccttg atgaagtcac tgaagcctat tacacaaccg gccactacag
     4501 catctttata aaagttatgt gccgttcgat cgacgctctc cagcatgtac ttatcaacaa
     4561 gatccaaaca attgatgaaa ttcagtccac cgagacattg atcgtcctgc aaaacccgat
     4621 catgcgtacc atcaagccct gatcggcttt tttaatccca tacttttcca caggtagatc
     4681 ccaacgcgtt cacagcgtac aatacgccac tcttaataaa ggtggcggtt tatggcagat
     4741 atcactctta tcagcggcag caccctcggc ggtgccgaat atgtagcaga acacctggct
     4801 gaaaagctgg aagaggtggg ttttaccacc gaaacgctgc acggtccgct gttggaagat
     4861 ttatctgcct cggggatctg gctggttatc agctccaccc acggtgccgg agatattccg
     4921 gacaaccttt ctcctttcta tgaagcattg caggaacaga agcccgatct ttctgcggtc
     4981 cgctttggca caatcggtat tggcagtcgt gaatatgaca ccttttgtgg ggctatcgat
     5041 aaactcgagg cagaactcaa aaattccggt gcaaaacaga caggcgaaac actgaagatc
     5101 aacattcttg atcacgacat tccggaagat ccggcagaag aatggctggg atcgtggatt
     5161 aatttactca aataagtata cagatcgtgc gatctactgt ggataactct gtcaggaagc
     5221 ttggatcaac cggtagctat ccaaagaaca accgttgttc agtttttgag ttgtgtataa
     5281 cccctcattc tgatcccagc ttatacggtc caggatcacc gatcattcac agttaatgat
     5341 cctttccagg ttgttgatct taaaaatcgg atccttgtta tccacagggc agtgcgatcc
     5401 taataagaga tcacaataga acagatctct aaataaatag atcttctttt taatacccag
     5461 gatcccaggt ctttctcaag ccgacaaagt tgagtagaat ccacggcccg ggcttcaatc
     5521 cattttcata ccgcgttatg cgaggcaatc accatgtttt atccggatcc ttttgacgtc
     5581 atcatcattg gcgggggtca tgcaggcacc gaggccgcga tggctgcggc gcgtatgggt
     5641 caacagactc tgcttttgac acacaatatc gacactctgg ggcagatgag ctgcaacccg
     5701 gcgatcggcg gtattgggaa gggacatctg gtaaaagaag tggatgcact cggcggtctg
     5761 atggcgaaag cgatcgatca agcgggtatc cagtttagga tactaaacgc aagcaaagga
     5821 ccggcggttc gcgctacccg agctcaggcg gatcgtgtgc gctaccgtca ggcggtacgt
     5881 acggcgctgg agaaccaacc gaacctgatg atcttccagc aggcggttga agatcttatt
     5941 gtcgaaaacg atcgcgtggt cggtgctgtt acccaaatgg gactgaagtt ccgtgccaaa
     6001 gctgtggtgc tcaccgttgg gacgttcctc gacggcaaaa ttcatatcgg tctggataat
     6061 tacagcggtg gccgtgctgg cgatccgcca tccattccgc tctctcgccg tttgcgtgaa
     6121 ctgccgctgc gcgttggtcg tctgaaaacc ggtactccgc cgcgtattga tgctcgaacc
     6181 atcgacttta gcgtgctggc gcaacagcat ggcgataacc caatgccggt attctcgttt
     6241 atgggcaatg cgtccctgca tccccagcag gtgccgtgtt atatcactca taccaacgag
     6301 aaaacccatg atgtgatccg cagtaacctc gatcgtagcc caatgtatgc aggggtgatc
     6361 gaaggtgtcg gcccacgcta ctgcccgtcg atcgaagaca aagtcatgcg cttcgcagac
     6421 agaaatcagc atcagatctt ccttgaaccg gaaggactga cctctaatga aatttatccg
     6481 aacggtatct ccaccagcct gccgttcgat gtgcagatgc aaatcgtccg ctctatgcag
     6541 gggatggaaa acgcgaagat cgtgcgtccg ggttatgcca ttgagtatga cttcttcgat
     6601 cctcgcgacc tgaaaccgac gctggagagc aagtttatcc aggggctgtt ctttgctggt
     6661 cagattaacg gcactaccgg ttacgaagaa gccgctgcgc aaggtttgct ggccggtctt
     6721 aacgctgccc gtctgtctgc tgacaaagaa ggttgggctc cggcacgttc tcaggcgtat
     6781 ctcggcgtac tggttgatga cctgtgcact ttaggaacca aagaaccgta tcgtatgttt
     6841 acttcgcgcg cagaatatcg tctgatgctg cgcgaagata atgcggatct gcgtttgact
     6901 gaaatcggtc gtgaactggg cctggtggat gacgaacgtt gggcgcgctt taacgagaaa
     6961 cttgagaata tcgagcgtga gcgtcagcgt ctgaaatcga cctgggtaac cccgtcggcg
     7021 gaagctgcag ccgaagtgaa tgctcacctg actgcgccgc tttcccgtga agccagtggt
     7081 gaagatctgc tgcgtcgtcc ggaaatgact tatgaaaaat taaccacgct gacgccgttt
     7141 gcccctgcgt tgacagacga acaggcggcg gaacaggttg agattcaggt taaatacgaa
     7201 ggttatatcg cgcgccagca agatgagatc gaaaagcagc tgcgtaacga gaacaccctg
     7261 ctacccgcga cactggatta ccgccagata tccggtcttt ctaacgaagt gatcgccaaa
     7321 cttaacgatc acaaaccggc ctctatcggc caggcttcgc gtatttctgg cgtcacgcct
     7381 gcggccatct ccattctgct ggtgtggctg aaaaaacagg gtatgctgcg tcgtagcgca
     7441 taacgcatta aaaatgcctg gtaagcaccc gcttaccagg caacgcatca agaacaggta
     7501 atcaccgtgc tcaacaaact ctccttactg ctgaaagacg caggtatttc gcttactgat
     7561 caccagaaaa accagcttat tgcctacgtg aatatgctgc ataaatggaa caaagcgtac
     7621 aacctgactt cggtccgcga tcctaatgag atgctggtac gccatattct cgatagcatt
     7681 gtggtggcac cgtatctgca aggtgaacgg tttatcgatg tcggcaccgg accaggactg
     7741 ccaggcattc cactctctat cgtgcgtcct gaagcccatt tcactctgtt ggatagcctt
     7801 ggtaaacgcg tgcgtttcct tcgtcaggtg caacatgagc ttaaactgga gaatattgaa
     7861 ccagtacaga gcagggtaga agagtttcct tcagagccgc catttgatgg cgtaattagc
     7921 cgcgcttttg cctctctgaa cgatatggtg agctggtgcc accatcttcc tggtgagcaa
     7981 ggccgtttct acgcgctgaa agggcaaatg ccggaagatg aaatcgcttt gttgcccgaa
     8041 gaatatcagg tcgaatcagt ggttaaactt caggttccag ccctggatgg cgaacgtcat
     8101 ctggtggtga ttaaagcaaa taaaatttaa tttttatcaa aaaaatcata aaaaattgac
     8161 cggttagact gttaacaaca accaggtttt ctactgatat aactggttac atttaacgcc
     8221 acgttcactc ttttgcatca acaagataac gtggcttttt ttggtaagca gaaaataagt
     8281 cattagtgaa aatatcagtc tgctaaaaa
//
LOCUS       DAEBXN010000158         8070 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.158, whole genome shotgun sequence.
ACCESSION   DAEBXN010000158 DAEBXN010000000
VERSION     DAEBXN010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8070
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.158"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(106..297)
                     /locus_tag="KJL99_003845"
                     /pseudo
     CDS             complement(106..297)
                     /locus_tag="KJL99_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            295..619
                     /locus_tag="KJL99_003846"
                     /pseudo
     CDS             295..619
                     /locus_tag="KJL99_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612087.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(649..3561)
                     /locus_tag="KJL99_003847"
     CDS             complement(649..3561)
                     /locus_tag="KJL99_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438412.1"
                     /translation="MFSKAFLRKISMFFARRKPAAMKICLYHTLNPDTIPGYKKFAQA
                     IATDNFVQADVRKIDTNLYRARLSIRDRLLFSLYRYHGETICLVLEYIRNHAYNTSRF
                     LRRNVVIDEGRLQQQPVPDPVDIATEALTYINPSHGRFHRLDKMLSFDDDQQALYEHP
                     LPLVIVGSAGSGKTALVLEKMKQAAGDILYLSLSSFLVEKARTLYDASGEGSEVQNID
                     FLSLTEFLETLRIPEGREVTFSAFSDWLPRNRAIAALGAAHTLYEEFRGVIGAVASGN
                     GPLSREAYLSLGIRQSLYGMEDRPTVYVLFERYIAWLKQSHQYDSNLLSHQYLSLATP
                     RYDVIFVDEVQDMTPVQLQLVLKTLRHPGQFLLCGDANQIVHPSFFSWSSLKSLFFRQ
                     QQGNDTTVNILQANYRNGHHVTALANRLLRLKQVRFSAIDRESHHFVRSCGQAEGTIR
                     LLDDREETKQELNAKTSLSNRVAVIVMHPEQKAQARCWFSTPLVFSVQEVKGLEYETV
                     ILYNIVSAARQAFDDICEGLTPADLEGEARYSRPRDRQDRSAEIYKFFTNALYVALTR
                     AKHNVYLVEQQVEHPLWSLLALTHQEEPLNLQEEISSRDEWQKTAHLLEKQGKQEQAD
                     TIRSRILQTSEMPWQIITAEDARQWKQHILAGTADKTIQLQALEYSLIYSLFPLYNAL
                     YREDFKPTRQPRTKTLQLLELKYFRPYSMNNPVAVLRDIERYGVDHRSPFNLTPLMSA
                     ARAGNIALVQLLLERGADPLLTGNDGLAAYHQVLSAAVSTPRYAQQKSAQLYTLLKPE
                     SLSLQVEGRLIKLDNRQMAMFLVILMQALFHTHLGSALFFSEAFSAARLAECVVHLPE
                     ALLPERRKRRSYISSQLSQHEVNSKNPYGKKLFLRLNHGQYILNPGLKIRKGDVWRAV
                     YELQSPEDLGHDLQTYLQDMSPELVDMLGGKKVFYERSEKSVGYWVGGIRRAAQKA"
     gene            3564..3680
                     /locus_tag="KJL99_003848"
     CDS             3564..3680
                     /locus_tag="KJL99_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBD4438413.1"
                     /translation="MPGIPPEIRWNNQKNALQLWNKEGGVVRREGDPLEMAQ"
     gene            4044..4886
                     /locus_tag="KJL99_003849"
     CDS             4044..4886
                     /locus_tag="KJL99_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBD4438414.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQIKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGREYLKEMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FYYLYTTERRMNAPAKGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     LPDD"
     gene            4889..5977
                     /gene="capU"
                     /locus_tag="KJL99_003850"
     CDS             4889..5977
                     /gene="capU"
                     /locus_tag="KJL99_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HBD4438415.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTR
                     KHPFRIIRQKTYLTRKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNDPGRFYQM
                     ARQAKQDIEERFDINKTALKILTLAKHK"
     gene            5982..6932
                     /gene="virK"
                     /locus_tag="KJL99_003851"
     CDS             5982..6932
                     /gene="virK"
                     /locus_tag="KJL99_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HBD4438416.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLQCIEDILAIQPTLPARIHRPYLHKGGRAWSRGQYILEHYRFVQNL
                     PEKYSEFLFPQKSVSLVQFIGKDGEDFDIQCSPSGFDREGELMLSLFFNKIVIARLTF
                     SVILTQNGHTAFIGGLQGAPKNTGPDIIRCATRACYGLFPKRIIFEAFCALMKACNVS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYKLPTQVVRKPLSN
                     IASKKRSEYRKRYALLDYIHETAIRSLDAYPVNSEHQDLN"
     gene            6997..7941
                     /gene="lpxM"
                     /locus_tag="KJL99_003852"
                     /gene_synonym="msbB"
     CDS             6997..7941
                     /gene="lpxM"
                     /locus_tag="KJL99_003852"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HBD4438417.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWFTWFVLGMIAGISMFPPSFRDP
                     VLAKIGRWVGRLSRKARRRATINLSLCFPEKSDTEREIIVDNMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGEKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHSREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHFLTIEVRPPMDACIASADNKMI
                     ARQMNKTVEILVGSHPEQYIWVLKLLKTRKSNEADPYP"
BASE COUNT         2171 a         1809 c         1925 g         2165 t
ORIGIN      
        1 ccggggcggt tcaaagggcc ttcccgagag tggtaaagga gaggccctgg aaatacccca
       61 cagccccgaa aaagagccag tcagttgctt aactgactgg cattaaggcc gccggggacg
      121 tttttttacc accgggagtt tgggcataac ggtgctttca gtaagtacga caggtactgt
      181 ggaggcactt ccgtaaagaa gggcatcaag ctcctgctcc gacatacctg caggcaaagg
      241 ccatgaaagc cctgatttcc ggaagcgagc gaacagtcca caaactgttg aacgtggtgt
      301 cagcggcgga cagggataag ccctcgtcag cagttccgtc agcactgcga cagcgttctt
      361 tacccggtca aaacagcgtt gcggtgcacc acgcctgacg gatgaactgc gtgctcaggg
      421 ttaccacttt aacgtaaaaa ccgtggcggc aagcctgcgc cgtcaggggc tgagggcaaa
      481 ggcctcccgg aagttcagcc cggtcagtta ccgcgcacat ggcctgcccg tgtcagaaaa
      541 tctgctggag caggattttt acgccagtgg tccgaaccag aagtgggcgg gagacatcac
      601 gtacttacgt acagatgaag ttcggttaca tcctgtcagc acggaacctc acgctttttg
      661 agcggcacga cggatccccc ccacccagta tccgacagac ttttcactgc gttcgtaaaa
      721 aaccttcttc cctcccagca tatccactaa ttccggtgac atatcctgca gataagtctg
      781 caaatcatgc cccagatctt ccggtgactg caactcataa accgcgcgcc acacatcacc
      841 cttccttatt ttaagccccg ggttaagaat atactgtccg tggttcagcc tcagaaaaag
      901 cttttttcca taaggatttt tgctgttaac ttcatgttga gagagctggc tgctgatata
      961 actccttcgt ttgcggcgtt ccggcagcaa cgcttcagga agatgaacca cacactctgc
     1021 cagtcgtgcc gcactgaagg cttccgaaaa aaacagagca cttcccagat gcgtgtggaa
     1081 cagcgcctgc atcaggatca ccagaaacat tgccatctgc cggttgtcca gtttgatgag
     1141 gcgtccttcc acctgcagtg acagactttc aggtttcagc agggtataca actgcgcact
     1201 tttctgttgc gcataacggg gcgtactgac cgccgctgac agaacttgat gataagccgc
     1261 cagcccgtca ttaccggtca gcaagggatc tgcaccacgc tccagcagta actgaaccag
     1321 tgcaatatta cctgcccggg ctgctgacat cagcggagta agattaaacg gcgaacggtg
     1381 atcaacacca taacgttcaa tatcccgcaa cacggcaacc gggttattca ttgagtatgg
     1441 ccggaaatac ttcagctcca gtaactgcag agtcttcgtt ctgggctgcc ttgtgggctt
     1501 aaaatcttcc cggtacagtg cattatacag cgggaacagg gaataaatca gactgtactc
     1561 cagcgcctgc agttgtatgg ttttatcggc tgtacctgcg aggatatgct gtttccactg
     1621 tcgtgcatcc tctgcggtga taatctgcca tggcatctca gatgtctgca ggatccggct
     1681 acggatggtg tccgcctgct cctgcttccc ctgtttttca agcagatgtg cggttttctg
     1741 ccactcatca cggctggaaa tctcttcctg cagattcaga ggctcttcct gatgcgtgag
     1801 cgcaagcaga ctccacagag gatgctccac ctgctgttca acaagataca cattgtgttt
     1861 agcacgggtg agcgcaacat acagcgcgtt ggtaaaaaat ttatagattt ccgctgaacg
     1921 atcctgcctg tcccgcggac gggaataacg ggcctcgccc tcaagatcag ccggtgtaag
     1981 accctcgcaa atatcatcaa atgcctgccg ggcagcgctt actatattgt aaaggatcac
     2041 cgtttcgtat tccagcccct taacctcctg aaccgaaaag accagcggcg tactgaacca
     2101 gcatcgcgcc tgtgctttct gttccggatg catcacaatg accgctacac gattcgaaag
     2161 tgaggttttg gcattcagtt cctgctttgt ctcttcccta tcgtccagca accggatggt
     2221 cccttctgcc tgaccgcagc ttctgacaaa atggtgactt tcccgatcaa tagcactaaa
     2281 gcggacctgt ttgagtctca gcaaccggtt agccagtgca gtgacgtggt gcccgttacg
     2341 gtaattcgcc tgcaggatat taacggttgt gtcgtttccc tgttgctgcc ggaaaaagag
     2401 acttttgagc gaactccatg agaaaaagct cggatgaact atctgattgg catcaccaca
     2461 gagtaaaaac tgaccgggat gacgtagcgt tttcagtacc agttgcagtt gaacaggcgt
     2521 catatcctga acttcatcaa caaaaatgac atcataacgg ggagttgcca acgaaagata
     2581 ctgatgactg agcagattac tgtcatactg atgcgactgt ttcagccatg cgatataacg
     2641 ttcaaacagg acatagaccg tcgggcggtc ctccatgcca tagagcgact gccgtatccc
     2701 cagtgacaga tacgcctccc gggacagcgg cccattcccg gaggcaaccg caccgatcac
     2761 gcccctgaac tcctcataca gggtatgggc agcccccagt gcggcgatag ccctgttgcg
     2821 gggcagccag tcgctgaacg cactgaaagt cacctccctg ccttccggaa tgcgcagagt
     2881 ttccagaaat tccgtcagcg aaagaaagtc aatattctgt acctcactcc cttcccctga
     2941 agcgtcatag agagtacgcg ctttttccac cagaaatgag gaaaggctga gatacagtat
     3001 atcaccagca gcctgcttca ttttttccag taccagcgcc gtcttaccac ttcccgccga
     3061 gccaacaatc accagcggta gcgggtgttc gtacagtgcc tgctgatcat catcaaagga
     3121 cagcatttta tccaggcggt ggaaacgccc atgagacgga ttgatatacg tcagcgcttc
     3181 agtcgcgata tcaacaggat ccggcactgg ttgttgttgt aagcgccctt catcaatcac
     3241 cacgtttctg cgaagaaaac gtgaagtgtt ataggcatga ttgcgaatat attccagaac
     3301 aaggcagata gtttcaccgt ggtagcgata cagcgaaaac agtaatcggt cccggatact
     3361 gagccgggcg cggtaaagat tggtgtcaat tttacgaaca tcagcctgaa caaagttatc
     3421 ggtggcaata gcctgtgcaa attttttata acccgggatc gtgtcaggat tcagagtatg
     3481 ataaagacag attttcatgg ctgccggttt ccgtcgtgca aaaaacatgc tgattttacg
     3541 aaggaatgcc ttactgaaca accttgcccg gaataccccc cgaaatcaga tggaacaatc
     3601 agaaaaatgc tctgcagtta tggaataaag aaggtggggt ggtacgccgg gagggcgatc
     3661 cgctggaaat ggcgcagtag ctgcaggcct gtcatgatgc aggaatagaa gttcgtgtac
     3721 aggttaatga aagcgtcacc ccgtaaataa actctgcgct gaaattcctt tttttcattg
     3781 gagtacctgt gttgttctga ggtgaacata tcacctctgt tcaggtagcc aaattcagtg
     3841 tgccacctca aggttcttag tgagtcgttt ttaaaagata tgataatata tcattgttta
     3901 tgaattgttg atgtttaatt tagtaaatta tatcaaatat gccatgggtt tgctatgtta
     3961 ttcatccagt atctggaaat aaaatgcacc agctttattg ggttttcctt ttgtctctgc
     4021 ttgctaggtg ccagaaactt taaatgttaa acgaaggggg cattttgttc aaagccaatc
     4081 atgttccggt attaatgtat catcatgtca gtcattgccc cggactggtt acactttctc
     4141 ccgtgacatt ccggaagcaa ataaaatggt tggctgaaaa caactggaaa acactgtcat
     4201 cagatgaact ggagtttttt tatcggggcg gaaaactccc ccggaaaagc gttatgttga
     4261 catttgatga tggatacctt gacaactggt ttcaggtata tcctttatta aaagagttta
     4321 atctcaaagc acatattttt ttaattaccg gttttattgg gaatggtccg gttcgtcact
     4381 ctcctggaaa ggaatattct catcgcgatt gcgaacatca aatagcgaca gggaatgctg
     4441 ataatgttat gcttcgctgg tctgaagtaa acgaaatgtt gcaatctggt ctggtcgaat
     4501 ttcatgttca tacgcatact catacacgct gggataaaaa gttcagttca cgagaagaac
     4561 agtgtaaaca tcttcggcag gacttgttga gcgggaggga atatttgaaa gaaatgacag
     4621 gaaaatgcag taaacatttg tgttggccgg aaggttatta taacaaggat tatattcagg
     4681 tcgctgaaga acttggattc tattatttat atacaacaga aagaagaatg aatgctcctg
     4741 ctaaaggaac tacacggata ggaagaataa gcaccaaaga acgggaaagt tgcgcatggc
     4801 tgaaaagacg acttttttat tatacaaccc cgtttttttc atcgttgctg gcctttcata
     4861 aggggccaag attaccggat gattaataat gaatatacta tttacggaat catcaccgaa
     4921 tataggtggt caggaattac aggctgttgc tcaaatgaag gccctgaaga aaatggggca
     4981 ttcagttctg cttgtctgca gggaaaacag caaaattgct tttgaagcca gtaaattggg
     5041 gattgatatc acattcgcgt tatttcgaaa cagtcttcac atccctactg catggagatt
     5101 actcggaata gttcatggtt ttcagcccaa tgcaatcgtt tgtcacagtg ggcatgatag
     5161 caatattgtt ggtttagtac ggttattcac taggaaacat ccattcagaa ttatcaggca
     5221 aaagacatat ttgacacgaa aaacaaaagt tttttcaata aatcattttt gcgatgaggt
     5281 gattgttccc ggaacaagta tgaagacaca tctggagcag gaaggatgtc gaacccgggt
     5341 tactgttgtg cctccaggct ttgatttcca gaaattatac gttgattccc gaaacagttt
     5401 gcctccaaat gttctttctt ggctggcgtc ccgaagggga tgccccgtta ttgctcaggt
     5461 aggaatgttg cgcccggaaa aagggcacga atttatgttg aatttactgt tccatttaaa
     5521 aatgaatgga cgacagttct gttggttgat tgtggggtct ggttcgcctg aactgcggga
     5581 gcatttacag tatcagattg acagtatggg catgcatgat gatgttttta ttgctgacaa
     5641 tgtttttcct gccgcccccg tatatcgggt tgccagtctg gtggttctgc cttcagaaaa
     5701 cgaatcgttt ggtatggtac tggcagaagc atcggcattt tctgtgcctg tactggccag
     5761 tcagattggt ggaatccctg atgttattca gaataaccag accgggacat tgttaccagc
     5821 aggtaataag cacgcatgga tgtgcgccct gaatgatttt tttaatgacc ctgggcgttt
     5881 ttatcagatg gctcgccagg caaaacagga tatagaagag cggtttgata ttaataaaac
     5941 tgcgttaaaa atactcacat tagcgaagca caagtaacaa tatgttttct gtaagtaact
     6001 tatcatttat cggtttcctt aaaaggattg ttttttcctc agattcactc ccggggaagt
     6061 gggaacacag aaaattccgg ttcatgtaca ttttgcgatg tgctataaat ccggttgcca
     6121 gtattcgata ttattacgaa ctgcgttcct tgcagtgcat tgaggatatt ctggcaatac
     6181 aacccacgtt gccagcaaga attcatcgac cttatttgca taaagggggg cgagcctggt
     6241 cccgggggca atatattctt gagcattatc gctttgtgca aaatttgcca gaaaaatatt
     6301 ctgaattttt gtttccacaa aaatcggtat cattagtcca attcatcggg aaagatggag
     6361 aggattttga tatccagtgt tctcccagtg gttttgaccg ggaaggagag ttgatgttgt
     6421 cattgttttt caataaaata gtgattgccc ggttaacatt ttcagtcatt ctgactcaaa
     6481 atggacacac cgcttttatt ggcggattac agggagcccc caaaaatacc ggacctgata
     6541 ttattcggtg cgcaacccgg gcctgttatg ggcttttccc aaaacggatt atttttgaag
     6601 cattttgtgc gttgatgaaa gcttgtaatg tctctgagtg tctggcagtc agtgagcaca
     6661 gtcatgtttt ccggcaattg agatactggt atcagaaacg caaaaccttt gttgctgtct
     6721 acagtgattt ctgggagtct gtagcaggga aaacctgtgg tgactggtac aaattaccaa
     6781 cacaggtggt tcgaaagccg ctaagcaata tagctagtaa aaaacgctct gagtatcgaa
     6841 aacgatacgc tttgctggat tatatccatg aaactgctat ccgctctttg gatgcatatc
     6901 ctgtaaactc agaacatcag gacttaaatt aaggtttgat gtattctctg aataaaatat
     6961 taatgatgat tttggtaggg gcattcgcac taaataatga aaaaatacaa atccgagttt
     7021 attcctgaat ttaagaaaaa ttatctttcc cctgtttact ggtttacatg gttcgttttg
     7081 ggaatgattg caggtatttc aatgtttccc ccttcattca gagatcctgt cttggccaaa
     7141 atagggcgtt gggtgggcag attgagcaga aaagctcgtc gcagggcgac gattaattta
     7201 tcgctttgtt tcccggaaaa gagtgataca gaacgggaaa taattgtcga caatatgttt
     7261 gccacagcat tgcaatccat agtgatgatg gcagaactgg caattcgtgg tccggaaaag
     7321 ttccagaaac gagtgttctg gaaaggcctc gaaattcttg aggaaattcg gcacaataac
     7381 agaaatgtga tttttctggt tccccatggc tggagcgtgg atattcctgc aatgttgctg
     7441 gcagctcagg gggaaaaaat ggccgccatg tttcatcagc aacgaaatcc agtgattgat
     7501 tatgtctgga attctgtacg gcgtaaattc ggggggcgct tacattcccg ggaggatggg
     7561 ataaaaccat ttattcagtc agtacgccag ggatactggg ggtattacct tccagatcag
     7621 gatcatggtc ctgaatacag tgaatttgct gatttttttg cgacctataa agcgacatta
     7681 ccaattattg gacgtctgat gaacatcagt caggctatga ttataccgct tttcccggtt
     7741 tatgatgaaa aaaaacattt cctgactatt gaagttcggc caccaatgga tgcatgcatt
     7801 gccagcgcgg acaataaaat gattgcccga caaatgaaca aaacagtgga aattttggtg
     7861 gggtcacatc cggaacagta tatctgggtt ttaaaattgt taaaaacgcg caaatcaaac
     7921 gaagcggacc cgtacccttg aaattataaa gtacaggtat ttccactagt tgtttcttac
     7981 aggttaccaa tcgaaacaca tcccccttcc gtgtgttgcc cgtttggcac ggaggactgt
     8041 gtagatgcag ttcgttaaat ttaatttatg
//
LOCUS       DAEBXN010000159         7982 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.159, whole genome shotgun sequence.
ACCESSION   DAEBXN010000159 DAEBXN010000000
VERSION     DAEBXN010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7982
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.159"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..159
                     /locus_tag="KJL99_003853"
     CDS             <1..159
                     /locus_tag="KJL99_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438418.1"
                     /translation="ISESGEVVDMAAYEAWQQENHPDTWQQMQRREEVNINVHRERGE
                     DVEPGDDF"
     gene            complement(168..566)
                     /gene="vapC"
                     /locus_tag="KJL99_003854"
     CDS             complement(168..566)
                     /gene="vapC"
                     /locus_tag="KJL99_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HBD4438419.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGKMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(566..793)
                     /gene="vapB"
                     /locus_tag="KJL99_003855"
     CDS             complement(566..793)
                     /gene="vapB"
                     /locus_tag="KJL99_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HBD4438420.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSTDFMDNREQPGMQERESF"
     gene            875..1246
                     /locus_tag="KJL99_003856"
     CDS             875..1246
                     /locus_tag="KJL99_003856"
                     /inference="COORDINATES: protein motif:HMM:NF020332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative relaxase"
                     /protein_id="HBD4438421.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSISMMAMLGGDKRL
                     IEAHNQAVDFAVRQVEASAST"
     gene            1267..6128
                     /gene="traI"
                     /locus_tag="KJL99_003857"
                     /pseudo
     CDS             1267..6128
                     /gene="traI"
                     /locus_tag="KJL99_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079803.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
     gene            6148..6894
                     /gene="traX"
                     /locus_tag="KJL99_003858"
     CDS             6148..6894
                     /gene="traX"
                     /locus_tag="KJL99_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HBD4438422.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSNRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVLC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            6949..7509
                     /locus_tag="KJL99_003859"
     CDS             6949..7509
                     /locus_tag="KJL99_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HBD4438423.1"
                     /translation="MTEQKRPVLTLKRKTEGTAPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAGARLAKIRRQNRIKAELQAVLDEK"
     gene            7640..7852
                     /locus_tag="KJL99_003860"
     CDS             7640..7852
                     /locus_tag="KJL99_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HBD4438424.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
BASE COUNT         1815 a         2029 c         2579 g         1559 t
ORIGIN      
        1 atcagtgaat ccggtgaagt ggtggatatg gccgcttatg aggcatggca gcaggaaaat
       61 catccggaca cctggcagca gatgcagcgt cgtgaagagg tgaacatcaa tgtgcaccgg
      121 gaacgcgggg aggatgttga gccgggagat gatttctgat gaacaggtca gctccagtct
      181 tcagttctca ggccgcccac acgttcaaat tcccgggtgt tattagtcac aataatcagc
      241 ccccgactgc gggcatgacc tgcgatcatt tgatcaaatg gcccgacagg gcgtccctga
      301 cgggcaagtt ctgctcttat ctggccggtg tgtgtggctg cagcagcgtc gtaatccaga
      361 acatcaatgc gggaaacaaa tccctcgatc acagcgagat tacgttcagg catctggctt
      421 ttttctgcac catatatcag ctccatcagg gtgaccgaac tgatacacat tttcccctgg
      481 ttcaggttaa aacgctccct gacgctggcg ggtttgttct ttatcgtaaa aatgcagatg
      541 ttggtatcga gcataaactt cagcatcaga atgactccct ttcttgcatg ccgggctgtt
      601 ccctgttatc cataaaatcg gtgctgacgc tgtgtccgtc gaaccattcg tcccatgtct
      661 ctccggctgg cgtaatgatt ctggttcgtc cgacagcaat cacttcaacg cgttttacgt
      721 tttccggcaa tgcaaccgct tttggcagtc tgaccgcctg gctgcggttg ctgagaaata
      781 cggtggtttc cattatttgc ctccttatgt ggatatgtct tgtgtatatc tattgtggga
      841 ttgataccgg gatatgtcaa agggatatac gtttatgatg agtatcgcgc aggtcagatc
      901 ggccggaagt gccggtaatt attataccga caaggataat tactatgtgc tgggcagcat
      961 gggagaacgc tgggccggcc agggggctga acaactgggg ctgcagggca gtgtcgataa
     1021 ggatgtcttt acccgccttc tggagggcag gctgccggac ggggcggatt taagccgcat
     1081 gcaggatggc agtaacaggc atcgtcccgg ctacgacctg accttctccg ctcccaaaag
     1141 tatctccatg atggccatgc tcggggggga caaacgtctt attgaagcac ataaccaggc
     1201 cgtggatttt gctgtccgtc aggtggaggc cagtgcctcc acctgacgga cggacagtca
     1261 gaaacggtgc tgaccggtaa tctggtgatg gcactgttta accacgacac cagtcgcgat
     1321 caggaaccac agttacacac gcatgcggtg gtgactaatg ttacgcagta taacggcgaa
     1381 tggaagacac tgagcagtga caaagtgggg aaaacggggt tcattgagaa tgtgtacgct
     1441 aatcagattg cctttggcag gctctaccgg gaaaaactga aagagcaggt tgaggcgctg
     1501 ggctatgaaa ctgaagtggt cggtaagcac ggtatgtggg aaatgccggg tgtaccggtg
     1561 gaggcctttt ccggacgctc acagactatc cgggaggccg tcggggaaga cgcctcgctg
     1621 aaatcccggg atgtggcggc gctggatacg cgtaaatcca aacagcacgt cgatcctgaa
     1681 gtcagaatgg ccgagtggat gcagacgctg aaggaaaccg ggttcgacat ccgggcatat
     1741 cgtgacgcag cggaacaacg ggcatatacc cgcacgcaga cacccggacc cgcttcacag
     1801 gacgggccgg atgtgcagca ggcggtgaca caggccattg ccggattaag tgaacgcaaa
     1861 gtgcagttta tgtatacgga cctgctggcc aggacggtcg gcatactgcc gccggaaaat
     1921 ggtgtgattg aacgggcacg cgccggtatc gatgaggcca tcagccgtga gcagcttatc
     1981 cccctcgatc gtgagaaggg gctgttcacg ttcgggattc atatgctcga tgagctgtca
     2041 gtccgggcac tcagtcgtga catcatgaaa cagaaccggg tgaccgtaca tccggagaaa
     2101 agtgtccccc ggacggccgg ttacagcgat gccgtcagtg ttctggcaca ggaccgtccg
     2161 tcgctggcca ttgtgtccgg gcagggcggt gcagccgggc agcgtgagcg ggtggctgaa
     2221 ctggtcatga tggcccggga gcaggggcgg gaggtgcaga ttatcgctgc tgaccgtcgc
     2281 tcgcagatga acctgaagca ggatgaacgg ctgtccggtg aactgataac cggacgtcgt
     2341 cagttgctgg aaggaatggc cttcacgccg ggcagtaccg ttatcgtcga ccagggcgaa
     2401 aaactctccc tgaaagagac gttaaccctg ctggatggtg ccgcacgtca taacgtacag
     2461 gtcctgataa ccgacagcgg gcagcgaacc ggtacaggca gtgcactgat ggccatgaag
     2521 gatgccgggg tgaacacata tcgctggcag gggggagaac agcgaccggc caccatcatc
     2581 agtgaaccgg accgtaatgt cggctggccg gagattttgc ggccagcgtg aaagccggag
     2641 aagagagcgt ggcacaggtc agcggagtac gggaacaggc catactgaca caggccattc
     2701 gcagtgagct gaaaacacag ggcgtgctcg gacacccgga ggtaaccatg accgccctgt
     2761 caccggtctg gctggacagc cggagccgtt atctgcggga tatgtaccgc ccgggaatgg
     2821 tgatggagca gtggaacccg gagacacgca gtcatgaccg ctatgtgaca gaacgtgtga
     2881 cggcgcagag tcacagcctg accctgcgga atgcgcaggg cgaaacgcag gtggtgcgta
     2941 tttcttccct ggacagcagc tggtcgctgt tccggccgga aaaaatgccg gtggcagacg
     3001 gtgagcgtct gagggtgaca gggaaaattc ccggactccg cgtctccggc ggtgaccgcc
     3061 tgcaggtggc atccgtcagt gaagatgcga tgacggttgt tgtgccggga cgggctgaac
     3121 cggccaccct gcctgtgagc gattcaccgt tcactgcact gaagctggag aacggctggg
     3181 tggaaacgcc cgggcattcc gtcagcgaca gtgcgacggt ttttgcctcc gtcacacaga
     3241 tggcaatgga caatgccacc ctgaatggtc tggcccgcag tggtcgtgat gtccggctgt
     3301 attcctcact ggatgaaacc cgtactgcgg aaaaacttgc ccgccatccc tcctttacgg
     3361 tggtttctga gcagataaag gcgcgtgccg gtgagacatt gctggaaacc gctatcagtc
     3421 tgcagaaagc cgggcttcac acgccggcac agcaggccat tcatctggcc cttcctgtgc
     3481 tggaaagtaa aaacctggcc ttcagcatgg tggacctgct gacagaggcg aagtcgtttg
     3541 ctgcagaagg aaccggtttt gctgacctgg gaggggaaat caatgcgcag ataaaacggg
     3601 gtgatttact gtatgtggat gtggcaaaag gctatggcac aggcctgctg gtttcccgtg
     3661 cgtcgtatga ggcagaaaag agcattcttc gccatattct cgaaggtaag gaggcggtca
     3721 cgccgctgat ggagagagta cctggcgaac tcatggagaa actgacatca ggacagcgcg
     3781 ccgccacccg tatgatactg gaaacgtctg accgtttcac ggtggtgcag ggctatgccg
     3841 gtgtgggtaa gaccacacag ttccgggcgg tgatgtcagc cgtgaacatg ctgccggaga
     3901 gtgaacgtcc ccgtgtcgtg gggctggggc ccacacaccg tgcggtcggg gagatgcgca
     3961 gcgccggcgt ggatgcacag acactggcgt cctttctgca tgacacgcag ctgcagcagc
     4021 gcagcggaga aactccggat ttcagcaaca cgctgttcct tctcgatgag agctctatgg
     4081 tgggcaatac cgacatggca cgggcatacg ccctgattgc ggccggtggc ggtcgggctg
     4141 tggccagcgg tgacacggac cagctgcagg ccatcgcgcc cggtcagcct ttccgtctcc
     4201 agcagacgcg cagtgctgcc gatgtggtca tcatgaagga gattgtgcgt cagacgccgg
     4261 aactgcggga ggcggtatac agcctgatta accgggatgt ggaaagggcg ctgtccgggc
     4321 ttgagcgtgt gaaaccgtct caggtgccac gtctggaggg cgcatgggca ccggagcact
     4381 ccgtgacgga gttcagtcac agccaggaag cgaaactggc agaagcgcag cagaaggcga
     4441 tgctgaaagg cgaggctttt ccggatgtcc ccatgacact gtatgaagcc attgtccgcg
     4501 actataccgg caggacaccg gaagcacggg agcagacgct gattgtcacg cacctgaatg
     4561 aggaccggcg cgtactgaac agcatgattc atgatgcacg ggaaaaggcc ggagaactgg
     4621 ggaaggtgca ggtcatggtg cctgtcctga acacagcgaa tatacgcgac ggggagctgc
     4681 gtcgtctctc cacctgggag aataacccgg atgcccttgc cctggtggat aatgtgtatc
     4741 accggattgc cggtatcagt aaggatgacg ggctgataac cctgcaggat gcggaaggta
     4801 acacgcggct gatttcgccc cgggaggcgg tggctgaagg cgtcacgctg tacaccccgg
     4861 acaccatccg ggtggggacc ggtgacagga tacgcttcac gaagagtgac cgggagcgcg
     4921 gttatgtggc caacagcgtc tggacggtga cagcggtttc cggtgacagt gtcacacttt
     4981 ctgacggtca gcagacccgt gtgattcgcc ccggtcagga gcgggcagag caacatattg
     5041 acctggccta tgccatcacc gcccacggtg cacagggggc gagtgaaacc tttgccatcg
     5101 cgcttgaagg cacggaaggt aaccggaaac tgatggccgg ctttgagtca gcctacgtgg
     5161 ccctgtcgcg tatgaagcag catgtgcagg tgtacaccga taaccgtcag ggctggacgg
     5221 atgccatcaa caatgccgta cagaaaggaa ctgcccacga tgtgtttgag ccgaaaccgg
     5281 accgggaggt catgaatgca gagcggctgt tcagtacggc gcgggagctg cgggacgtgg
     5341 cggcaggccg tgccgttctc cgtcaggcgg ggctggccgg gggagacagt cctgcacggt
     5401 ttattgctcc gggacgtaaa tatccgcaac cgtatgtggc actgccggcg tttgaccgta
     5461 acggcaagtc cgccggtatc tggctgaacc cgctgaccac ggatgacgga aacgggctgc
     5521 ggggattcag cggtgaaggt cgggtaaaag gcagcgggga tgcgcagttc gtggccctgc
     5581 agggcagccg taacggagaa agcctgctgg ctgataatat gcaggatggc gtccggattg
     5641 cccgtgataa tcctgacagt ggtgtggtgg taagaatcgc cggtgaaggc cgtccgtgga
     5701 atcccggtgc catcaccggt ggccgcgtgt ggggggatat cccggacaac agcgtccagc
     5761 cgggagccgg aaatggtgaa ccggtcacgg cagaggtact ggcacagcgg caggctgaag
     5821 aggccatccg ccgtgaaacg gaacgtcgcg cagatgaaat tgtccgtaaa atggcagaga
     5881 acaaacctga cctgccggac ggcaaaacag agcaggctgt cagggaaatt gccgggcagg
     5941 agcgtgaccg ggctgccata actgaacggg aagccgcgct gccggagagt gtactgcgtg
     6001 aaccacaacg ggtgcgggaa gcggtccggg aagttgcccg ggaaaatttg ctgcaggagc
     6061 gtctgcagca gatggagcgg gatatggttc gtgacctgca gaaagagaaa accccgggcg
     6121 gagactgata caggaagaca aacactgatg acaacggata acacgaacac gacacgtaac
     6181 gattcactgg ctgcacggac cgatacctgg ttgcagtcat ttctggtctg gtcacccgga
     6241 cagcgggata tcatcaaaac ggtggcactg gtgctgatgg tactggacca catcaatctg
     6301 atattccagc tgaagcagga atggatgttt ctggccgggc gaggagcctt tccgcttttt
     6361 gccctggtgt gggggctgaa tctgtcccgt catgcgcata tccggcaacc ggccattaac
     6421 cggctgtggg gatggggaat tattgcccag ttcgcatatt atctggcagg ctttccttgg
     6481 tatgagggga atatcctgtt tgcctttgca gtggcggcac aggtgctgac gtggtgtgaa
     6541 acgcgcagtg ggtggcgtac agcagcggcc atactcctga tggccctgtg ggggcctttg
     6601 tccggcacca gttacggtat tgccgggttg ctgatgctgg cagtcagcaa ccggctgtac
     6661 cgggcggaag acagagcgga acgtctggcg ctggtcgcct gcctgctggc tgttattccg
     6721 gcgcttaacc ttgccaccag tgatgcagcg gcggtggccg gtcttgtgat gacggtgctg
     6781 accgttggtc tggtgttgtg tgccggaaaa tcattacccc gtttctggcc cggagacttt
     6841 ttcccgacgt tctatgcctg tcatctggct gtgctgggcg ttctggcgct gtgacgggtg
     6901 tggtatcttt ggccgcaaga ggatgattcg tcagaggcag aacacagcat gacagagcag
     6961 aagcgaccgg tactgacact gaagcggaag acagagggaa cagcgcctgt ccgcagccgg
     7021 aaaaccatca tcaatgtcac cacgccacca aagtggaagg tgaaaaagca gaagctggcc
     7081 gagaaagcgg cccgtgaagc agagctggcg gcaaaaaaag cgcaggccag acaggcgctg
     7141 tccatttatc tgaacctgcc cacgctggat gaggctgtga ataccctgaa gccctggtgg
     7201 ccgggattat ttgacggtga cacgccccgg cttctggcct gcggtatccg ggacgtgtta
     7261 ctggaagacg tggcgcagcg gaatatcccg ctctcgcata aaaaactgcg cagggcgctg
     7321 aaggccatca cccgttcaga aagctatctg tgtgccatga aagccggtgc ctgccggtat
     7381 gacacggaag ggtatgtgac ggagcatatt tctcaggagg aggaagcgta tgcgggagcg
     7441 cgtctggcga aaattcgtcg ccagaaccgg ataaaggcag aacttcaggc cgtgcttgat
     7501 gagaaataaa aaaagcctcc cccagagaga ggaggcaggg aaataaggct taaaaggaaa
     7561 taacttccgc ttccgaaagg aagccagtcc actgaattat ttatcacaat aatactgaca
     7621 actaaagggg gacaagaaaa tgaatggatt cagaaacagt tcacggaacg gccaggtctg
     7681 gcgttaccag cgtgccggcg ggcgggccgt tattctggaa gtcagtggac gctggatgga
     7741 agcggcagaa gcatggcgac gggctgcctg tgttgcgcct aggacagact ggcagcagtt
     7801 tgcccggaaa agggctgaac actgccaccg gcgctgccgg ggaagggtgt aacgaaaaaa
     7861 gcagccttca ttactctagg agtcagaagg ctgaaaggag tttatgagat ttgcttctta
     7921 atatccggtc gggcagtatg accggacagt tctgagtagc acagagtctt ttccgcatta
     7981 ag
//
LOCUS       DAEBXN010000160         7942 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.160, whole genome shotgun sequence.
ACCESSION   DAEBXN010000160 DAEBXN010000000
VERSION     DAEBXN010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7942
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.160"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(54..533)
                     /gene="xdhC"
                     /locus_tag="KJL99_003861"
     CDS             complement(54..533)
                     /gene="xdhC"
                     /locus_tag="KJL99_003861"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HBD4438425.1"
                     /translation="MNHSETITTECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGTVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(530..1408)
                     /gene="xdhB"
                     /locus_tag="KJL99_003862"
     CDS             complement(530..1408)
                     /gene="xdhB"
                     /locus_tag="KJL99_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HBD4438426.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATFIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(1419..3716)
                     /gene="xdhA"
                     /locus_tag="KJL99_003863"
     CDS             complement(1419..3716)
                     /gene="xdhA"
                     /locus_tag="KJL99_003863"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HBD4438427.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALTEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRICSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            4131..4886
                     /locus_tag="KJL99_003864"
     CDS             4131..4886
                     /locus_tag="KJL99_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HBD4438428.1"
                     /translation="MSAGRLNKKSLGIVMFLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            4965..5038
                     /locus_tag="KJL99_003865"
     tRNA            4965..5038
                     /locus_tag="KJL99_003865"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4997..4999,aa:Gly,seq:ccc)
     gene            5173..6789
                     /locus_tag="KJL99_003866"
     CDS             5173..6789
                     /locus_tag="KJL99_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBD4438429.1"
                     /translation="MLNSRTYLYQRNGVFYIRLRMKTTGRLTASLPSHNRYKLASVSL
                     RTKDRRTAMAHSRHIKSALKAIHADNPNASYEELREHLKTIVEWELSVSRDDLNDPES
                     YQLYVDQYDDIKSNLREAVATERLTVDQHRYINDVIGVLKGCQDRLKGDSSGLLSYLE
                     PETSSLRPSVSLSVSSEPEVPEPKALTLASLIEQYEQENAQNWKPATLSENRASHSTL
                     IEILDYLDIQDVGKATRADMLRVREVLQLLPKNRKQRFKTMPLSDLLNRESKNDCLDV
                     VTINNKYLIKMAAVFKWAVRNDLIAKNLTEGLELKVPQRKASDARGAFSPEQVRQLLV
                     AAKAYSQKTAGKPYHYYVTALAAVTGARLNEVAQLQVKDIRVTEAGTVYIHINEDDSS
                     LPGKSIKNAHSDRCVPLVDGAYGFVLADFMSLVEDRRKTEGDNAMVFDGLKLMKNGYG
                     EQVSKWFNRTLIPKVLADRSGLAFHSFRHTVATQLKQHGVELAYAQAIMGHSSGSITY
                     DRYAKEVEVDRLVNVMAGVYKETGVDGDTV"
     gene            6773..7429
                     /locus_tag="KJL99_003867"
     CDS             6773..7429
                     /locus_tag="KJL99_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HBD4438430.1"
                     /translation="MAIPFEAPEFEKEAFNCPYCNAFAHQKWQQLYYYYSSTGGLPVH
                     TSTCEHCEEKSFWFQRRLLIPFASTAELPNPDMPDNCKADYMEARSIVGLSPKGAAAL
                     LRLCLQKLMVHLGEPGNNINADIRSLVQKGLPVRIQQAADICRIVGNQAVHPGEISLD
                     DDPQLAHGLFKLLNIIVDDRITRPKEIEAMFQSMPEGPRQGIENQDRQAREQQQAANE
                     "
BASE COUNT         1987 a         1984 c         2047 g         1924 t
ORIGIN      
        1 tgttaaacac ataacctcag tgaatccggc ctgaattcag gccggatatc cttttacttc
       61 gttttctcgc aatccagaac tgtatttaca atcatctgat accccgtgca gcgacaaaga
      121 tttcccgcca gtccgcgacg aatttccgta atggttaatg gcttctcgcg tggtttcgcc
      181 agcattgccg tggtagccat aatcaggcca ggcgtacaaa acccgcactg cactgtgccg
      241 gatttcgcat aagcctgctg aacatgagaa agttttccgc ctttcgcttc accttccagc
      301 gtgcggatct cttttccttc agcccaggcg gcaaggtata agcaactgtc tattgctgtg
      361 ccgtcgacca acaccgtaca ggcaccacat tcacccacgc agcacccttg tttgacactt
      421 agcagtcctt gttcgcggag taattccgag agcggcgtgc ctggtgcggc gtgaagctga
      481 aaaggcatcc cgttaatggt gcattcggtg gtaattgttt cgctgtgatt cattgcaatt
      541 ttccccccgc cgcggcgacg gcttcgctaa tcactttttt ggtcatcgtc tggatgagat
      601 gcagacgaaa ctctttactg gcccgccatg aagaacgcgg ggcgacatct tgcaggacag
      661 attcgctgat agcttccagc gtttgcaggt ttaatggcgc attttgtgca gtctgttcgg
      721 catgttggca gcgaatcggc gttggcgcgg caacaccaaa tgccaggcgt aattcgctga
      781 aattgccgtt atccagtcgg caatgtgcgg cgcagccaat cgttgaaata tccattgcgt
      841 cgcgcatggc atatttaaaa tgcgcgctgc ccgcgtgttc tttcggctgt ggcggaaaat
      901 gaaaggcgac gaggatttcg tcatgctcaa gagacacttt gcccggcccg gtgtgaaagc
      961 cattaatcgg gacgaaacga acaccgcgcg gggagtggat ctccagtttc gcgtcataaa
     1021 ttagcgttgg cgtggcagaa tctgcgctgg tggcaccgtt gcaaatattt ccaccgtagg
     1081 tagcgacgtt acggatctgc ggtccagcaa tgaacgtggc cgcagcacat aacgccggga
     1141 gatgacgttg agttatagga tcttctatta gctgggtaaa tgtcgttgca gagccgatac
     1201 gtagcgagcc atcttccgcc agcgtaattc cccgcagctc cgccagatta tgaatatcaa
     1261 caatatggcg ataacggtca ttgtgatggt ggagctgaat cagtacgtca gtgccaccgg
     1321 cgagcagttt ggcctgcggg ttgtcagcca gcaggttgat ggcatcggca agggttgctg
     1381 cgcgatggta agaagcaaaa tcaaacatga tgttatcctc aaatcaatcc tgccagatgg
     1441 aactcttcat ataaccgttt tggcgtcagc ggcagtgtat tgattgcaac accggtagcc
     1501 atcttcaccg cgttacgaat agcagcggca acaggaatta tgggtggctc acccagtgac
     1561 ttatgtccgt atgcggattg cggctcattg atttcgacga acgcgctttc cagttgtggc
     1621 agatccggca tggtcggcat tttgtaatcc agcagattgg ggttacggac cacgccgctt
     1681 ttagcatcga tgatcatctc ttcaaatagc gcccagccaa tgcccattcc cattccgccg
     1741 tgtacctgac cttctgccag cagtggatta agaatatgcc ctgaatcatg aacgttgagg
     1801 atgcggttga tggtgacttt gcacagcgca atatcgaccg tcagatcaac aaaggtacag
     1861 ccaaacgccg gtgggttagt ggtggttttg atggagcttt cagcagagag ctgcccgccg
     1921 cgttcagggt ggtagaaagc gtccatcgcc aaatctttta acgacattaa cggctcttcc
     1981 ggtcgttcaa ccagcacgat atggcctttt atcagggtca aattcatcgc tgactgatgt
     2041 agcatgactg cggcgtgagc gatgattttc tcttttaata atagtgccgc actgcgcagc
     2101 gcaggcgcgg caacatagct ctggcgtgag gcaaatgcgc cgggatcgaa cggcgtaacg
     2161 tcggtatctt gtgttgaaat aacgcgaacg tcgctgaccg gaacccccac ggtttctgcc
     2221 accatttgcg agaagacggt gtcggcaccc tgaccgattt ccgtcgcgcc gctttgcacg
     2281 ttgatggttc catcctgatt catcagaagg cgcgcgcctg ctatttctac gccgacaggc
     2341 caggtgttag aggtgtagct aaaacaggcg acgccaacgc cgcggcgcaa attgccttgc
     2401 tggttctggc attctgcacg gcgtttttcc cattcaaaga ttttccggcc tttttcaaga
     2461 cactccggca accctgcgct gcaaatacgt ttgcccgtga gcggattagc atctccttcg
     2521 cgggcggcgt tgcgtaaacg aatttcaaca ggatcaatac ctaacgctgt cgcggcgtca
     2581 tcaagcatag actcaacggc aaatacgact tgtggcgcgc cataaccacg catcgcacca
     2641 gccgagggga ggttggtata gcaggtcttt gaactgtaag cgtaggcaca acgaggataa
     2701 aggtaagcga ctttattccc ccccgcagaa gcgatggagt gcccgtgaga tgcataagcg
     2761 ccggtgttag acagaacatc cagactataa cctttcaagg ttccgtcgcg gttcacgccc
     2821 atttgcccgt caatagtaaa agcgtggcgg gtacgggttg cgaggaaaca ctcttcacgg
     2881 ctaagggaaa ctttcaccgg aatgccgcca agcttgctgg tcaggaatgc cgccattggc
     2941 tcttccagta catcctgttt attaccaaaa ccgccaccga caaacggttt gatgactcgt
     3001 acgcatgacc agggaatatc cagcgcctga ccaaccacgc ggcgaacaat gtgcgggatc
     3061 tgggtgctgg aaacgatggt aattcgcgag tcatcctcca tccacgccag cgatgttacg
     3121 ctttccatgt gacaatgttg aataacggga gtctgatagt gcccctgtac ctggtagtcg
     3181 gcggcatcga ttgtttgttg gacattgccc gtcgacatcg tgctttgttt cagtaaattg
     3241 ccgccgttat ggattggtgc agcgtcttct gtcagcgccg cttctggcgt ggtgataacg
     3301 ggtaattctt gccactcaat gctgaccaat tgcgccgctt tttctgccgt gagttcatcg
     3361 cgggccacga cgatggcaac ggcgtcacca tgatgacgaa catggcgagt tagcagtgcg
     3421 cgatcggcgg tatcgcgctt gttttcgtca agtgtccagg catgcccagc tgtagcgaat
     3481 ggaatatcag gcacatcttc ccaagtaaaa atcgccagta cgcccggtaa acttctggct
     3541 tgttcatcat taatacttac ggcataacca tgtgcgatag ggctacgtac atatttcgcg
     3601 taacacatgc ccgccataac ataatcgtca gtatatcgtg cccgcccggt gaccttagca
     3661 atggcatcga cgcgcatgca tgattcaccc gtagcggttg cttcccgcgc ttccatagaa
     3721 atcccctcga tttgaacatt tggttcttat ggcaaagatt tagtctaagc aggcaagatt
     3781 tacgccagaa acgacgataa gcgttagggg aggaaggaag tgtgatgacg ttaaaaaaac
     3841 acagatatga tacgaaacag gactctgaat agggacagtg atacgtatca atatgagtgg
     3901 cgataaaatc aatgtgataa atcaggatta ttacgcatca ggatgggaaa tagggtaaaa
     3961 agtcaggaac ttcgatacct ctacagcgaa tattttgctg atgatacttc cctttgccaa
     4021 atgaaattaa cctctttctc tattaccacg tttttccaga agcaagagat tgggtatcaa
     4081 gaggctggct gctatgatat ggcgtcttgt tttttaagcg aggaaagatt ttgagtgcgg
     4141 gacgcctgaa taaaaaatct ctgggtatcg tgatgttttt atcggttgga ctgctattgg
     4201 cgggctgttc gggtagcaaa tcatccgata caggaacgta ttccggctcc gtttacaccg
     4261 tgaaacgggg ggatacgcta tatcgtattt cgcgcaccac gggaaccagc gtaaaagagc
     4321 tggcgcgact gaacggcatt tccccccctt acaccattga agttggtcag aaactaaaac
     4381 tgggtggggc gaaaagtagc agtagtacac gtaaatcaac cgccaaatca acgactaaaa
     4441 ccgcatcggt tacaccgtca tcagcggtac cgaaatcttc ctggccgcca gtagggcaac
     4501 gttgttggtt atggccaacg acagggaaag ttatcatgcc gtattcgaca gcagatggcg
     4561 gcaataaagg gattgatatc tcagctccac ggggtacacc tatttacgcc gcgggtgcag
     4621 gaaaggtggt gtatgtgggc aaccagctgc gtggctacgg taatctcatc atgattaaac
     4681 acagtgaaga ttacattacg gcttacgccc ataatgacac gatgctggta aataatgggc
     4741 aaagcgtgaa ggctgggcaa aaaatcgcca ctatggggag cacggatgcg gcatctgttc
     4801 gcctgcattt ccagattcgt taccgtgcaa cggcaattga tccgctacgt tacttgccgc
     4861 cgcagggcag caagccaaaa tgctgatggc gaattaatca gcagtcagca gcgcggcact
     4921 tgctaaggag agcgtaaggt ttataatgcc ttacgcatct cgaagcgggc gtagttcaat
     4981 ggtagaacga gagcttccca agctctatac gagggttcga ttcccttcgc ccgctccatg
     5041 acctttctta aagtctacta acttactgaa tcctaaggga catggcttaa agccttccga
     5101 tagttttact cactgtaacc aacactgtga ctaactttgt tgacgtagtg agcagaccta
     5161 aggtctcagt acatgctcaa ctcaagaacc tacctttatc agcgtaacgg tgtcttttac
     5221 atccgtctta ggatgaagac cactggtcgc ctgaccgctt cgcttccttc tcacaaccgt
     5281 tataaactag cgtcagtatc actacggacc aaggatagac gtaccgctat ggctcactca
     5341 cggcacatca agtcagcact taaagcaatc cacgcagata accctaacgc ctcttatgag
     5401 gagctacggg aacacctgaa gaccattgtc gagtgggaac ttagtgtcag ccgcgatgac
     5461 ctgaacgacc cggagtccta ccagctttac gttgaccagt acgatgatat taagtccaac
     5521 cttcgggaag ctgtagcgac tgagcgtctg actgtagacc agcatcgcta tatcaatgac
     5581 gttatcggcg tacttaaagg ctgtcaggat agactcaagg gcgatagctc tggcctgttg
     5641 tcttaccttg agcctgagac cagtagtcta agaccatctg tctccctatc tgtatcatcg
     5701 gagcctgaag tccccgaacc taaagctctg accctcgcgt ctctcattga gcagtacgag
     5761 caggagaacg ctcagaactg gaagccagcg accctaagcg agaaccgagc gtcgcactcc
     5821 acgctaatcg agatattaga ctatctggat attcaggatg tcggcaaggc gacccgtgca
     5881 gatatgctca gggtccgtga agtcctccag ctgttaccga agaaccgcaa gcaacgcttt
     5941 aagaccatgc cgctgtctga cctgctgaac cgggagtcta agaatgactg tctagatgtc
     6001 gttaccatca acaataaata tcttatcaag atggctgctg tgtttaagtg ggcggtacgc
     6061 aacgacctga tagctaagaa cctgacagaa ggtcttgaac tgaaagtccc tcagcgtaaa
     6121 gcctccgacg ctcgtggcgc gttcagtcct gagcaggtaa ggcaactact ggtcgccgct
     6181 aaagcgtact ctcagaagac cgcaggtaag ccataccatt attacgtcac cgctctagct
     6241 gcggttacag gtgcgagact taacgaggtg gcccagcttc aggtcaaaga catcagggtc
     6301 actgaggccg ggactgtgta tatccatata aacgaagacg atagcagcct accgggtaag
     6361 agcatcaaga acgctcatag cgaccgctgt gttccgctag tagatggcgc ttatggtttt
     6421 gtattggctg actttatgtc gcttgtggaa gaccgtagaa agactgaggg tgataatgct
     6481 atggtattcg atggcctcaa gctgatgaag aatggctacg gtgagcaggt tagtaaatgg
     6541 tttaaccgga cgttgatacc taaagtcctt gctgaccgta gcggcttagc gtttcattcg
     6601 tttaggcata cagttgcgac ccagttaaaa caacatgggg tcgagttagc gtatgctcag
     6661 gcgattatgg gtcatagctc agggtctata acttacgacc gttacgcgaa agaggtcgag
     6721 gtagatagat tggttaatgt gatggcgggt gtttataagg agactggagt agatggcgat
     6781 accgtttgag gcacctgagt ttgaaaaaga agcctttaac tgcccatatt gtaacgcttt
     6841 tgcacatcaa aaatggcagc agttgtacta ttactattca agtactggag gcctccctgt
     6901 tcatacttct acctgtgagc attgtgaaga aaaaagtttt tggtttcagc gccgcttact
     6961 tataccgttt gcttccacag ccgaactacc aaacccggac atgcccgaca actgtaaggc
     7021 tgactacatg gaagccagaa gcatcgttgg cctgtcacct aaaggggccg ctgcactcct
     7081 tcgtctatgc cttcagaagc taatggtcca cttaggcgaa ccggggaata acattaacgc
     7141 tgacatccgg tcactcgttc agaaaggact acctgtacgc atccagcagg cagcagacat
     7201 ctgccgtatt gtcggcaatc aggcggtcca tcccggagag attagccttg atgatgaccc
     7261 gcagttggct catggcctct ttaagctgct gaacattatt gtcgatgacc gaatcacgag
     7321 accgaaagaa attgaggcta tgttccagag tatgcctgaa ggtccacgcc aaggtattga
     7381 gaatcaggac agacaggcga gggaacagca gcaggccgct aacgaataac ccctcactat
     7441 aggggaaggg gacttaacta tcactatagg gagagaccct aagtcaccag cctgctggca
     7501 ttctccgaca cacatataca ttcattaagt ctcaggcttc cggtagcgga ccgattggaa
     7561 accatccgca gacgcctagt gttaaatcac ctggcctgag accattctct ttaacgtagg
     7621 ttatcttccc ttttgacagg cggggagcag aagaccgcat ccgactttaa gttggctctg
     7681 cgataatcgc tgcgcggata actgaagcac caagacatca ttgctgaggt ctgccatcca
     7741 tttgtggaca ggcggaaact caaagtggtt gaccagtgga gtctgaggtt ggactttgag
     7801 tcctgaccag caggctggag gagaccatga gtttatcttg ttatggattg tgttatcaca
     7861 attccaataa gaagaacttt aagtccagcc tccagtcctg tcagcccgaa gtaaactatg
     7921 gtcgctatga tatggtctcc at
//
LOCUS       DAEBXN010000161         7865 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.161, whole genome shotgun sequence.
ACCESSION   DAEBXN010000161 DAEBXN010000000
VERSION     DAEBXN010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7865
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.161"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(175..660)
                     /gene="sixA"
                     /locus_tag="KJL99_003868"
     CDS             complement(175..660)
                     /gene="sixA"
                     /locus_tag="KJL99_003868"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HBD4438431.1"
                     /translation="MQVFIMRHGDAALDSASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(863..3007)
                     /gene="fadJ"
                     /locus_tag="KJL99_003869"
     CDS             complement(863..3007)
                     /gene="fadJ"
                     /locus_tag="KJL99_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HBD4438432.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(3007..4317)
                     /gene="fadI"
                     /locus_tag="KJL99_003870"
     CDS             complement(3007..4317)
                     /gene="fadI"
                     /locus_tag="KJL99_003870"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HBD4438433.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPTVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAYVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4497..4781)
                     /locus_tag="KJL99_003871"
     CDS             complement(4497..4781)
                     /locus_tag="KJL99_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HBD4438434.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKSRSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5153..6493
                     /gene="fadL"
                     /locus_tag="KJL99_003872"
     CDS             5153..6493
                     /gene="fadL"
                     /locus_tag="KJL99_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HBD4438435.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAIYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            6859..>7865
                     /locus_tag="KJL99_003873"
     CDS             6859..>7865
                     /locus_tag="KJL99_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438436.1"
                     /translation="MLPSISINNTSAAYPESINENNNDEINGLVQEFKNLFNGKEGIS
                     TCIKHLLELIKNAIRVNDDPYRSNINNSSVTYIDIGSNDTDHITIGIDNQEPIELPAN
                     YKDKELVRTIINDNIVEKTHDINNKEMIFSALKEIYDGDPGFIFDKISHKLRHTVTEF
                     DESGKSEPTDLFTWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGD
                     SLTQYCSENARSASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRA
                     IRYNFQVDDAKFRRDNVKEIISTLFANKVDVDHPENKYKDFKNLEDKVEKRLQNRQTK
                     Y"
BASE COUNT         2106 a         2003 c         1803 g         1953 t
ORIGIN      
        1 taactttggg caaaatcaag atacgcgtag aagaataatt tccgaatgaa tgtagaaagt
       61 gttttattta tagataagga gagtcttcga tagtagtgag gttgacagat atccgctgcc
      121 taatgagcaa ttatgcccaa tgaatctacc tcattgggca taatttggaa cacatcagat
      181 agcttttgcc atcttcagat tacacgggct catctgccag ttgaatgtac cgtttccgct
      241 ttcatcaagc gttacgctgg caatggccga ggtggtaaac ataggcggcg tttcgcccgg
      301 acataactcg gcaaccagat acccgactaa cggtaagtga gagatcacca gtaccgaagc
      361 aacaccttca ttggtcaacg cctgcaaata ggcactgacc agaccaacat cgccgcaggg
      421 cgttaactcc ggcagaactt ctgcactgga aggcaggttc agacaatccc ctacttcttc
      481 cagtgtttgc tcggctcgca ggaacgggct caccagaacg cgttcgattt ccactttttg
      541 acctttcagc cagttcgcca tcaggcgaga ttcgtcacaa ccattagtgg tcagaggacg
      601 aaccgaatca ctggcggaat cgagggctgc gtcgccgtga cgcatgataa aaacttgcat
      661 attgcaccgc ttttgttaac cagattcgcc cgttttcttt catctgaaac cagagaaaga
      721 aaacggtggc cggcattgtg ccttatccat tcaccgaatg aaacgctgtt ttttacctca
      781 atggcgtaag tatagtcaat atgcgtttac attttgacca acactccgcc attcagcgcg
      841 gattcatata gctttgacct tcttattgca ggtcagttgc agttgttttc caaaaacttt
      901 ccccacgcgc gcccatctcg accaaacgct cgcaaggggt aaaacgggaa ccatactgcg
      961 tggccagtcg ttgcattatt gcaaccactt cgcccgcgcc gagagaatcg atatagcgga
     1021 acggtccacc gagaaatggc ggaaaaccaa tgccaaatac cgcaccaata tccccgtcac
     1081 gcacgctacg gataacctgc tcatcaacac aacgtactgc ttcattcagc atcaacatca
     1141 cacaccgttc agcaacctgc ggtgcggaga gtcgcccctg cccttgtgcg ccaatgagcg
     1201 ggtaaatggc aggatcgacc tgttttttgc ttttacgccc tttctgacca taaagataga
     1261 aaccccgacc attttttctg cctttgcgat cgtcgttcaa aattgaagaa acaacatttg
     1321 caggcgcgct aaaacgttct ccataagcgg cttccagtac aggaataatt ttagtcccgg
     1381 tgtcgattcc tacctcatcc aaaagttgga ttgggcctac cggaaaacca aatttcacta
     1441 gcgcggcatc aatgtgctct acccgttcac cttcggtcag catgcggata gcttcattaa
     1501 tgtaaggcgc taagatgcga ttgacgtaaa acccggcttt gtcacgcacg acaatgggcg
     1561 ttttgccctg ttttttcgcc agttttaccg tagtggcgat ggtttgcgcc gatgtacccg
     1621 catgaggaat aatctctacc agcggcattt tttccaccgg actgaagaaa tgcaggccaa
     1681 taacttgctc aggtcgcgcg gcgtgagcgg cgatatcacc aatcggtaaa gatgacgtgt
     1741 tcgaagcaaa aatggtatga gcggcgcaat tttgctccac ttccgccacc atctgttgtt
     1801 ttaattcgag attttcaaac accgcttcaa taatcagatc gcgatgggca aagccgcgat
     1861 agtccgtcgt tccggagatt aatgccagct gtttgtcacg ttcgctggct ttgagatgac
     1921 gacggcgaac tttgccctcc agctgatccc aactgtactt cagcgcatga tttatgccct
     1981 gcgggttgat atctttaatt ctgaccggaa gccccgcttt acaagcagtg acataagcaa
     2041 taccgccgcc catcaagcca ccacctaaaa ttcccacgct gtttaatggc gcaggcggtg
     2101 catcactacc gggatctttc ttcacgtccg tactggcaaa aaagatacta cgcaaagctt
     2161 gcgattgcgg cgtcatcgcc agttcgccaa aggcgcgagc ttcagcgtca tagccgctgc
     2221 tggtgccctg cgctaatccc gtttcaacaa cctccaggat gcgttctgtc gccggataat
     2281 tgccttgagt tttgtgttct gttttcttgc cgaccatttt gaacagcagc gcacgaccta
     2341 acggccccgc cagaatacgc tcgcgtacag gtagagggcg ggaagatggg cgatccttct
     2401 tcgccagctc aacagcggct tccagcaaaa tggagtgcgg aacaacgtca tccaccagcc
     2461 ccagctttaa tgcctgtttc gcccgaagtt gttttccggt gagaatcatc tctaatgctg
     2521 tactgacgcc tatcagacgc ggtaaacgct gggtgccgcc tgaaccgggt aacaatccaa
     2581 gttgtacttc aggcaaaccg agcaccgttt taggatcgtc agtacaaacg cgaccgtggc
     2641 acgccagcgc cagctccagc ccaccgccca ggcaagcgcc atgaatagcc gcgataaccg
     2701 gaatgggcaa agcatgaatc tccgccatca actgttggcc ctgccgcgcc agtgcttccg
     2761 cttcttgcgc cgttttgcag ttgccaatca tgttgatgtc tgctccggcg ataaaattgt
     2821 ccggtttcgc ggagataaac accacgccgc gcaactcttt gttttcacgg agttgcttaa
     2881 taatggcgcg cacctgcgag gcaaattccg ccttcagggt attcattttc tcatccggta
     2941 cgtcgatggt gatgatagca atgttgtcca gacgaacatt aagagtaaac gctgatgcca
     3001 tttccattat tccgcctcca gaaccattgc cgcgccaagc ccaccggcag cacaggcggt
     3061 aactaaacca aatccaccgc cgcgacggcg aagttcatgc aatgtctggg taatcatccg
     3121 cgcgccggtc gccgcgaagg gatgtccgta agcaatcgaa ccgccaagca cgttaaattt
     3181 gctatcgtcc acttcgccag tggcatgtgc acgccccagt acttcacgag caaaacgttc
     3241 actacccagc aactgaatat tcgccagtgt ctgagctgca aaggcttcgt gcatatcgat
     3301 caatgtcagt tcgctcatcg tcaaaccggc acgttccagc gccagcggtg ttgaccaggc
     3361 tggaccgagc aacatgtcct gccagacatc aatcgcagta aatgcgtagc tgcgcagata
     3421 ccccagcggc accagcccta attctttcgc ccgggattca gtcatcagga tcaccgctgc
     3481 cgcgccatcg gtcagcggcg tactgtttgc cgccgttacc gttccgtgtt tgcgatcaaa
     3541 cgccgggcgc agctttgcgt aatcggcaag cgaggaatta ccgcgaatat tgttgtcttc
     3601 gacaagcggt tgtttataag gagggataaa ggcagtcatc acctcttctt tgagttttcc
     3661 gtctgaccat gcctgagcgg cacgctgatg cgaacggtgc gctaatgcat cttgctgttc
     3721 tcgggtgatg ccgtaggttt tcgccatttg ttcggcggtg tcgcccatcc gcaagccagt
     3781 agaatattct gctaccgcag gcggtacggg cattaagtcg cgcaaacgca ggcgagagaa
     3841 gagtttcagg cgctggctca tggttcgagc tttgttgaca tcaaccagca cgtacgccag
     3901 ttttttactg acgccaattg gcaatacgga agaggaatct gccccaccgg caatccccgc
     3961 tcggatagtt cccgccatca ggctttctgc gacgtttgca accgcttgga aactggtagc
     4021 gcaagcgcgg ctgacgctgt aagcatcggt atgcacattc attccggtac cgagaacaat
     4081 ttcacgcgca atgttggggg cttcaggcat ttgtacgacc tgaccaaaga ccagctgttc
     4141 aatcacttca gcggggatct cgctgcgtgc cagcagttcg cctaccacca tcttccctaa
     4201 atcaaccgtg ggaatgccat gaaaagccgt cgcctggcgg gcaaaaggcg tacgtaaacc
     4261 gctaacaatg gcgatacgat cgccctggcg ggtaaccagt ggtaaaacct gacccataac
     4321 actcccctgt aaaaaaataa ataaagtggt ctgacctgat catagtctta accatttttt
     4381 tacatttagc caagcggaga aaaaggaaag tgggagctat gacacagtga agacagaaga
     4441 aagaaaagaa aacgcccctg ccagcaactg acaggggcgt acgtacccga gaggaattaa
     4501 cgcagaccca gctggaagat cagcatttca gcttcgcagg caaaagtgaa atcgatatcc
     4561 aggcgcacac cgtcagactc ttcagtgaaa gtcggggaga ttttgcacgg ttcggattcc
     4621 acgctacggg atttttcagt cagcgccgcc agcgtttgtt ctgcttctgc gcggtttgca
     4681 aacacgcggc tgtaagacgc ggtgcagtcg gagttgtcca taatggtgcc aacatccata
     4741 cagcagcaaa ccggggtttc atcagcacta catttactca tcgttgattt cctctgtatg
     4801 tgcacccaag gtgccagata aacgttgtgg atattttacg cttccggaaa gtgctgctcc
     4861 agttgttaat tctgcaaaat cggataagtg accgaaatca cacttaaaaa tgatctaaaa
     4921 caaaattcac ccgaatccgc gagtgcgcaa cctccaaatt ttgccagctg gatcgcgttt
     4981 cttagatcat atttgaaaaa agatggaaac atacttgcaa cattctagct ggtccgacct
     5041 atactctcgc cactggtctg atttctaaga tgtacctcag accctacact tcgcgctcct
     5101 gttacagcac gtaacatagt ttgtataaaa ataaatcatt gaggttatgg tcatgagcca
     5161 gaaaaccctg tttacaaagt ctgctctcgc agtcgcagtg gcacttatct ccacccaggc
     5221 ctggtcggca ggctttcagt taaacgaatt ttcttcctct ggcctgggcc gggcttattc
     5281 aggggaaggc gcaattgccg atgatgcagg taacgttagc cgtaaccccg cattgattac
     5341 catgtttgac cgcccgacat tttctgcggg tgcggtttat attgacccgg atgtgaatat
     5401 cagcggaacg tctccgtctg gtcgtagcct gaaagccgat aacatcgcgc ctacggcatg
     5461 ggttccgaac atgcactttg ttgcaccgat taacgaccaa tttggttggg gcgcttctat
     5521 tacctctaac tatggtctgg caacagagtt taatgatact tatgcaggcg gctctgtcgg
     5581 gggtacaact gaccttgaaa ccatgaacct gaacttaagc ggtgcgtatc gcttaaataa
     5641 tgcatggagc tttggtcttg gtttcaacgc catctacgct cgcgcgaaaa ttgaacgttt
     5701 cgcaggcgat ctggggcagt tggttgctgg tcagattatg caatctcctg ccgggcaaac
     5761 tcctcaaggg caagcattgg cagctaccgc caacggtatc gacagtaata ccaaaatcgc
     5821 tcatctgaac ggtaaccagt ggggctttgg ctggaacgcc ggaatcctgt atgaactgga
     5881 taaaaataac cgctatgcac tgacctaccg ttctgaagtg aaaattgact tcaaaggtaa
     5941 ctacagcagc gatcttaatc gtgcgtttaa taactacggt ttgccaattc ctaccgcgac
     6001 aggtggcgca acgcaatcgg gttatctgac gctgaacctg cctgaaatgt gggaagtgtc
     6061 aggttataac cgtgttgatc cacagtgggc gattcactat agcctggctt acaccagctg
     6121 gagtcagttc cagcagctga aagcgacctc aaccagtggc gacacgctgt tccagaaaca
     6181 tgaaggcttt aaagatgctt accgcatcgc gttgggtacc acttattact acgatgataa
     6241 ctggaccttc cgtaccggta tcgcctttga tgacagccca gttccggctc agaatcgttc
     6301 tatctccatt ccggaccagg accgtttctg gctgagtgca ggtacgactt acgcatttaa
     6361 taaagatgct tcagtcgacg ttggtgtttc ttatatgcac ggtcagagcg tgaaaattaa
     6421 cgaaggccca taccagttcg agtctgaagg taaagcctgg ctgttcggta ctaactttaa
     6481 ctacgcgttc tgataacgcg ttcgcctgga taaagtcacc tgctatgcag gtgactttaa
     6541 ctcccccact tcaccgaaag tagtttcctc attgcttgcc tcctgagttt tgcaaacagc
     6601 ctgatggcag cttgcccttt caaatcagta agtggtatcc acagacaaac ctgaagggaa
     6661 atgcatattt tcaagcgttc tgctcgtcct tctcaaagag tttacttttc tgcatttcca
     6721 ggatactcaa cccccctggc tattgtgcgc ttatacactc aaattaaaga taggttctaa
     6781 ataaatgagc gttttttgat agtctatttc attaggtaat atatatttgt aacaaatcaa
     6841 tcaacatgga ataaaatcat gctaccatct atttcaatca acaataccag cgcagcttac
     6901 ccagaatcca tcaatgaaaa taacaatgat gaaattaatg gattagtaca agagttcaaa
     6961 aaccttttta atggtaagga aggaataagc acctgtatta aacatctact tgagcttata
     7021 aaaaacgcca tacgagtaaa cgacgatcct tatagatcta atattaataa ttcctcagtt
     7081 acttatattg atattggctc caatgataca gaccatatta ctattggtat cgacaaccaa
     7141 gaaccaatag aattacctgc gaactataaa gacaaagaac tcgtccgtac tatcattaat
     7201 gacaacatag ttgagaagac tcatgatatc aataacaagg aaatgatctt cagcgcatta
     7261 aaagaaatat atgatggaga tcctggtttt atcttcgata agatatcaca caaactcaga
     7321 catacggtaa cggaatttga tgagagcggg aaaagcgaac caacggactt atttacctgg
     7381 tacggtaaag ataaaaaagg cgactctctc gctattgtaa ttaaaaataa aaacggaaat
     7441 gattacttat ctctcggtta ctacgatcag gacgactacc acattcaaag aggaattcgt
     7501 attaatggtg atagtctcac ccaatattgt agtgaaaacg ccaggagtgc ttcagcgtgg
     7561 tttgaaagca gtaaagctat catggcagaa tcatttgcaa ctggttccga tcatcaggtt
     7621 gtaaacgagc tcaacgggga aagactgaga gaacctaacg aagtttttaa acgtttaggt
     7681 cgagcaataa gatataattt tcaagtggac gatgcaaaat ttagacgcga taatgtaaaa
     7741 gaaataattt ctactttatt cgctaacaaa gttgacgttg accatcctga aaataaatac
     7801 aaagacttta agaatctcga agataaagtt gaaaaaagac ttcaaaatcg ccagacgaaa
     7861 tatca
//
LOCUS       DAEBXN010000162         7721 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.162, whole genome shotgun sequence.
ACCESSION   DAEBXN010000162 DAEBXN010000000
VERSION     DAEBXN010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7721
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.162"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(<1..830)
                     /locus_tag="KJL99_003874"
     CDS             complement(<1..830)
                     /locus_tag="KJL99_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase family protein"
                     /protein_id="HBD4438437.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFDNYVSTTLREKTQALYRSTVMKRMHDAFPNRPASSITVKQWVDLLTEEEKDNPRR
                     ARQVLSQLRSAISWCMRRQLIDSCAIMSIQPRDFGSRAEVGDRVLSYHELAKIWLAIE
                     RSRASTSNKLLHQMLMLWGARLSELRLATKTEFDLLDNVWTVPKEHSKMGNVIRRPIF
                     "
     gene            1012..2436
                     /gene="sbcB"
                     /locus_tag="KJL99_003875"
     CDS             1012..2436
                     /gene="sbcB"
                     /locus_tag="KJL99_003875"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HBD4438438.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPRPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLAHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMADLAGDISPLLELDID
                     TLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRHQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
     gene            complement(2479..2706)
                     /locus_tag="KJL99_003876"
     CDS             complement(2479..2706)
                     /locus_tag="KJL99_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HBD4438439.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(2720..3778)
                     /gene="yeeE"
                     /locus_tag="KJL99_003877"
     CDS             complement(2720..3778)
                     /gene="yeeE"
                     /locus_tag="KJL99_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HBD4438440.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISAQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVTVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGVTSPTANILQFLVAGDVKYINWGVFLVLGIFVEAFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(3957..5315)
                     /gene="plaP"
                     /locus_tag="KJL99_003878"
     CDS             complement(3957..5315)
                     /gene="plaP"
                     /locus_tag="KJL99_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HBD4438441.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANVNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(5305..5367)
                     /gene="yoeI"
                     /locus_tag="KJL99_003879"
     CDS             complement(5305..5367)
                     /gene="yoeI"
                     /locus_tag="KJL99_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HBD4438442.1"
                     /translation="MGQFFAYATVITVKENNHVA"
     gene            complement(5528..6511)
                     /locus_tag="KJL99_003880"
     CDS             complement(5528..6511)
                     /locus_tag="KJL99_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBD4438443.1"
                     /translation="MKPLLDVLMILDAIEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAIQRKIIMTSLSFSCHFPPI"
     gene            complement(6557..7381)
                     /locus_tag="KJL99_003881"
     CDS             complement(6557..7381)
                     /locus_tag="KJL99_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBD4438444.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLQPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(7464..7718)
                     /gene="yoeB"
                     /locus_tag="KJL99_003882"
     CDS             complement(7464..7718)
                     /gene="yoeB"
                     /locus_tag="KJL99_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HBD4438445.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
BASE COUNT         2068 a         2013 c         1811 g         1829 t
ORIGIN      
        1 tcgaagattg gacggcggat aacattaccc atcttgctat gctctttcgg tacggtccat
       61 acgttgtcca gcaggtcaaa ttctgtcttt gttgccagcc taagctctga gagcctcgcc
      121 ccccacagca taagcatctg atgaaggagc ttatttgacg tagacgcacg gcttctttca
      181 atagcaagcc aaatcttagc cagttcgtga tacgacagta cccgatcccc tacctcagcg
      241 cgggagccga agtcccttgg ctggatgctc ataattgcgc aactatctat caactgacgc
      301 cgcatgcacc aactaattgc tgatcttagt tgacttagca cctgccttgc tcggcgtgga
      361 ttatcttttt cttcttcggt aagcaggtca acccattgct taaccgtgat agaagatgcc
      421 ggacgattag ggaaggcgtc atgcatgcgc ttcataaccg ttgatcgata aagtgcctgg
      481 gtcttttctc tgagagttgt agagacgtag ttgtcgaacc agtagtcgag acactgggcg
      541 accgtcatgg agttctccac cttctcttca aaataggtgc gtggatccgt tcctgagaaa
      601 tagagctttc gcaagtcagc agtgatctgt ctggcatcct tcaaagacag ggatgggtat
      661 cgaccaagcc caagtcgatt aggcttgcca tgccagcgat agcggtactg gaactggatg
      721 acccccttcg gtgaaattcg tacgctgagg ccatcggcat cagccacttc ttgtgggccc
      781 gaatatggtt taccataaat agtacgcagt tttgtgtcgc ttatagccat aaataatatt
      841 tggtacgctc agaaataata gtttggcaca catctggtac acaatatcac atggacaaca
      901 gcgcacagta aacaactata tttgacgttc gtagacatac tcaggtgaaa aaagggttgc
      961 ttttcgagtt ataacagaca gttaattaac tacagtggac aatgctagac aatgacagac
     1021 acagataaac aacctacctt cctctttcac gattacgaaa cctttggcac gcaccccgcg
     1081 ttagatcgcc ctgcacagtt cgcagccatt cgcaccgatg acgaattcaa tgtcatcggc
     1141 gaacccgaag tcttttactg caagcccgcg gatgactatt taccccggcc tggagcagta
     1201 ttaattaccg gtattacccc gcaggaggca cgggcgaaag gagaaaacga agccgcgttt
     1261 gccgcccgta ttcactcgct ttttaccgta ccgaagacct gtattctggg ctacaacaat
     1321 gtgcgtttcg acgacgaagt cacacgcaac gttttttatc gtaatttcta cgatccttac
     1381 gcctggagct ggcagcatga taactcgcgc tgggatttac tggatgttat gcgtgcctgt
     1441 tatgccctgc gcccggaagg aataaactgg cctgaaaatg atgacggtct accgagcttt
     1501 cgccttgagc atttaaccaa agcgaatggt attgaacata gcaacgccca cgatgcgatg
     1561 gctgatgtgt acgccactat tgcgatggcg aaactggtaa aaacgcgtca gccacgcctg
     1621 tttgattatc tctttaccca tcgtaataaa cacaaactga tggcgttgat tgatgttccg
     1681 cagatgaaac ccctggctca cgtttccgga atgtttgggg catggcgtgg caataccagc
     1741 tgggtggcac cgctggcgtg gcatcctgaa aatcgcaatg ccgtaattat ggcggatttg
     1801 gcaggagaca tttcgccatt actggagctg gatatcgaca cattgcgcga gcgtttatat
     1861 accgcaaaag ccgatcttgg cgataacgcc gccgttccgg ttaagctggt gcatatcaat
     1921 aaatgtccgg tactggccca ggcgaatacg ctacgcccgg aagatgccga ccgactggga
     1981 attaatcgtc agcattgcct cgataaccta aaaattctgc gtgaaaatcc gcaagtgcgt
     2041 gaaaaagtgg tagcgatatt cgcggaagcc gaaccgttta caccttcaga taatgtggat
     2101 gcacagcttt ataatggctt tttcagtgac gccgatcgtg cagcaatgaa aattgtgctg
     2161 gaaaccgagc cgcgtaattt accggcactg gatatcactt ttgttgataa acgaattgaa
     2221 aagttgttgt tcaattatcg ggcacgcaac ttcccgggga cgctggatta tgccgagcag
     2281 caacgctggc tggagcaccg ccaccaggtc ttcacgccag agtttttgca gggttatgct
     2341 gatgaattgc agatgctggt acaacaatat gccgatgaca aagagaaagt ggcgctgtta
     2401 aaagcacttt ggcagtacgc ggaagagatc gtctaatttg aagccgccgc cgctggtacg
     2461 gcggaggaca atgttgagtc aggctttttg aacggtgatg ctccacgctg catcgccaat
     2521 ctgctgataa tcggtgatgg catgtccttc ttctgccgcc cactgtggga ttgcttcagt
     2581 tgcctgggtg caatcaaact caattaccaa ctcatcgccg ctggccattt ctgccagtgc
     2641 tgcttttgct tctatgagtg gaaacggaca gacctgcgtc accacatcca gctttttaat
     2701 cgccatcttt acaccttact taatttgccg cagcagttgc cagtcgcgcc ttacgctgcg
     2761 gtcgaacata cacaagccag gacgcagtcc agactccgag aatcataaat acaaggccaa
     2821 tccagccctg ccaggtcatc attgcagtca taaccagtcc attaccgata gagcaaccac
     2881 ctgcaatact ggcaccgaag cccatcagta caccacctaa cccgctacgt aatgttgttt
     2941 gtgcatcagc tgcgcgaacg cggaactcac ggctcgcttt ggctgcaata aatgcctcca
     3001 cgaagatccc taacaccagg aaaacgcccc agttaatgta tttcacgtca cccgcgacca
     3061 gaaattgaag aatattggcc gttggggaag tgactcctaa cccaaacatg cgtccggttg
     3121 cttcactcag gggccaggct aaaagcgcaa tcaaaccgat gagtacagca gtgacaaagg
     3181 gatgccagcg tttttcaaac agaatatgag cgatcccggt tcggcgcggc ggtaaggtcg
     3241 cgactttgag ttttggcttc ttcaactctt tcatcaccac ccagagcgtt atcaccagca
     3301 aaacggtaac caacggccac acagacaaat taaaagtctc agcaatagag ttatgttcag
     3361 tagtgtagtg ctgcaaggtt tgatttaaac cactggcgtg tggagaacgc atcaccgcac
     3421 tcatcaccat ataagtgaaa agcgcgatcc aactgccgat caatccctca cccgcgcgat
     3481 accaggtccc ggtggcacat ccacccgcca gaacaattcc cagcccgaaa agatacccac
     3541 ctataacagt accaagccac gggaacgccc cggcttcgta agtcagtagg cccgcctgaa
     3601 ttaacgcaaa aacccccacg ctttgtgccg aaattgcaat cagcaaggcg taaaacatgc
     3661 gattattttt cgcgatatac atatcgcgaa aaccaccagt caggcaaaaa cgcccacgct
     3721 gcatgacaaa tcccagcaga gcaccacaaa ttagcccgct taatatcatt gaaaacataa
     3781 attaattaac cagatgaatg ttaatgggga aatttttcat gactggtgga atgcagacca
     3841 ataaccaaat tctctaaatt agaacaaatg gttattaatg aggatttcga taaaaaagaa
     3901 taaaaaaacc ggagccaaat gttcattcga ctccggttta ttattagaaa agatgattat
     3961 gctacgtctt cgtactgcgg aaccggatta cggaagctct tggtgacgca agccaggtaa
     4021 atcagaccga tagcagccca gatcagaccc agaaccattg agctttcttc caggttaacc
     4081 cacagcgcac caacggtcag cgcaccacac atcggcagga acagatactg gaagtgatct
     4141 ttcagcgtct tgttacgctt ctcacggatc cagaactgcg agatgaccga caggttaaca
     4201 aaggtgaacg ccaccagcgc accaaagtta atcagcgccg ttgccattac caggtcgaag
     4261 ttgattgcca gcagggcaat cgcgccaacc aggatgatgt tcatcgccgg agtacgccat
     4321 ttcgggtgta cataaccgaa gaagcttttc gggaacacgc cgtcacgacc cattacgtac
     4381 atcagacgcg ctacgcctgc gtgcgccgcc ataccggacg ccagtacggt aatggtggag
     4441 aagatcagcg cgccaacctg gaacgcttta cccgcaacgt acagcatgat ttcaggctgt
     4501 gacgcatccg gatctttaaa gcgagagata tccgggaagt acagctgcag gaagtaagtt
     4561 gcaaagatga agatcatgcc gccaatcagc gcggtcagga aaatcgcacg cgggatcacg
     4621 cgctctgcat ctttggtttc ttccgacaga ttgctgatgc cgtcaaagcc ggtaaaggag
     4681 aagcacagga ttgtcgcccc ggtaatcatc gggataacat gcgcatcgcc agaccagaac
     4741 ggacgcgtgc tcgccagcgt accagcgcct tcaccttcaa atacgccata aacaaccatg
     4801 cccagaatca ccgcgatcag cactacctgc aacacgacga ttacagtgtt gacgttcgct
     4861 acggatttca gactacgcag gttaaaggcg gtcataaagg ccaccagcgc caccacaaac
     4921 atccacgatg gaatggaagg caccagagct tcaaaataga ttttcgccag cagaatgttg
     4981 atcatcggcg cgaacagata gtcgagcaga gaagaccaac ccaccataaa gccgacagtc
     5041 gggctaatgg atttctgggc gtaagtgtat gcagagccag cagaaggata gcggcgaacc
     5101 agcttcccgt agctcagagc cgtaaacagg atcgcaatca atgcgaacgc ataggctgtc
     5161 ggcacatgac catccgtgag gcctgaaacg ataccaaatg tatcaaacag cgtcatcggc
     5221 tgcatatagg ctagacccat cattacaacc ggaactaacg taagcgtttt acgcaattcc
     5281 acgcgagagg tgtttggagt aacgttatgc gacatggtta ttctccttta cggtgataac
     5341 cgtcgcgtaa gcaaaaaatt gccccatttt tttggattcc tcagcgacaa caactgtcga
     5401 tttttagtaa atatctatcc ggtacgaagc ccggcctctt ggtatgaatg attggtttga
     5461 agcaaaaaat aaccgacgct aattaaacgt cggtttttaa tcattttttg acagcggcgc
     5521 attgtgccta aatggggggg aaatgacaag agaatgagag gcttgtcata ataatttttc
     5581 tttgaatggc tgattttccg tcatcagatt ttgcataaac accggcgatc gcactatttg
     5641 ctaaaatttc atctcgccac cagccgctgg caagccctgc ggtctgttca ttccagccaa
     5701 tccagacagg ttcaaagaga gtttgggcga ccactttctt ctcgattaac gcgccactat
     5761 cgagaaaacg ttgcgccaga taccgtggta aatagccgca gccaaggccg ctaatttgca
     5821 gctccagttt ggttttaaaa tcgaaaacgg taatggcttc ttgttcgtcg agaagttgtg
     5881 aggccgtaga agcgcctgcc tgcgcagtat cccccaccac aatcgcccga tatcgtttaa
     5941 tgatacgacg gtttaacggt tcttcttcct gggctaacgg atgatgaggc gcgaccgcaa
     6001 agacttgttc aagatcgccc aaccgcgaga aaccaaactc actggaagat ggtggctcat
     6061 gcatcgcgcc aacgataata tccgctcgcc cctgagtcag ggcatcccag gaaccaccga
     6121 gtacgccatt gataaacttc aggcgcgtca cgctatgatg ttgataaaaa gcttcaatga
     6181 gaggcgcaag cagagaaaaa ggaaacgtgt cgtccacgcc aatcaccagc tcgttttccc
     6241 agccttcatg cagtttgatc gcctgctttt ccagttctcg cacggtatgc agaacttccc
     6301 gccctttctc taataacatt tttccggtgc gggtgaattt agcgcggtgt ccgctacgat
     6361 caagcaattg aatatttagg tcgctttcca gcttgtgaac ggtgtaactc agagcggacg
     6421 gcgtcttgta gagtttggct gacgccgccg caaaactgcc ctctttttct atggcatcga
     6481 ggatcatcaa cacatccagc agtggtttca tacacgcccc cttgcagtgc atgcggcgtc
     6541 ctgctggcgt gatcgtttac tccaacggca ttaccagcgg atcgggatac tggtattcaa
     6601 atcccagttc attacaaatc cgactgccat caataatctt acctttgccg ctgtccagac
     6661 tatttctgaa ctgcggaggt tgcagcccca gtaaacgggc catctgcgga tagaaaacat
     6721 tacgcgcagg gtgagctggc gcacatatat tatagatgtg tccgccttta ggtgcctgta
     6781 acaacagagt gatagcgcca ataacatctt ctaaatggac taaattaaca ccatgttcac
     6841 catcaggggc ggtttttccg gcaaagaagc gtcccggatg acgtcccggt ccgaccaggc
     6901 ccgcaagacg cagaatatcg accgaagtac cgggtaaatt gtgcagccag tcttcgagtt
     6961 cttctaatac tcgtccactg ttggttaccg gattacgcgg ggtagtttct ttaaccgtgc
     7021 cttgcgcgtc gccatagaca gatgttgagc tggtaaaaat aattcgggga atacgatggg
     7081 ccagcgcgct atccactaac tcttgtaccg cttgtaaata gaactcatcg ccggggccgc
     7141 tacgacgtgc cggaagcgta atgaccagcg catcggcatc cataagggca tccagatcgt
     7201 cagaatcgca aactaactca ggctccatgc gaagcagata gctatcaatg ccactcattc
     7261 gggccgcttc gacaccatct tgtgtggttt tactcccggt gacttgccag cctcgcgctg
     7321 aaagtgacat cgccagcggc atacctaacc accctaaccc gacaattgcg acctttttca
     7381 tccgttatct cctgatacac cttagatcta taaggctacg ctagcgtatc aaaactgaca
     7441 attcattcta tgaatgaatc tgttcaataa tgataacgac atgctgcaat gagcagtgaa
     7501 tcatcagtaa ccgcgtatac cagacggtgc tcctctgtaa tgcgtcggga ccagaaacct
     7561 gacaaattat gtttcagggg ttctggtttc cccttacctt caaatggtgt tctgcgggta
     7621 tctttgataa gttcattgat ctttttaaca attcgcttat ctgtttcctg ccagtacaga
     7681 taatcatccc atgattcctc agaccagatt agtttcactc a
//
LOCUS       DAEBXN010000163         7673 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.163, whole genome shotgun sequence.
ACCESSION   DAEBXN010000163 DAEBXN010000000
VERSION     DAEBXN010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7673
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.163"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            1..1158
                     /gene="rbsA"
                     /locus_tag="KJL99_003883"
                     /pseudo
     CDS             1..1158
                     /gene="rbsA"
                     /locus_tag="KJL99_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
     gene            1163..2128
                     /gene="rbsC"
                     /locus_tag="KJL99_003884"
     CDS             1163..2128
                     /gene="rbsC"
                     /locus_tag="KJL99_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HBD4438446.1"
                     /translation="MITQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALTLGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            2153..3043
                     /gene="rbsB"
                     /locus_tag="KJL99_003885"
                     /pseudo
     CDS             2153..3043
                     /gene="rbsB"
                     /locus_tag="KJL99_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            3169..4098
                     /gene="rbsK"
                     /locus_tag="KJL99_003886"
     CDS             3169..4098
                     /gene="rbsK"
                     /locus_tag="KJL99_003886"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBD4438447.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPSLVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            4102..5094
                     /gene="rbsR"
                     /locus_tag="KJL99_003887"
     CDS             4102..5094
                     /gene="rbsR"
                     /locus_tag="KJL99_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HBD4438448.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(5060..6487)
                     /gene="mdtD"
                     /locus_tag="KJL99_003888"
     CDS             complement(5060..6487)
                     /gene="mdtD"
                     /locus_tag="KJL99_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HBD4438449.1"
                     /translation="MSDKKKRSMSGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGDAMMMPVARPALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRIYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSMPNRVPSESE"
     gene            complement(6510..7202)
                     /locus_tag="KJL99_003889"
     CDS             complement(6510..7202)
                     /locus_tag="KJL99_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709569.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBD4438450.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWITEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         1961 a         1855 c         2012 g         1845 t
ORIGIN      
        1 aaccatgtat gtcgaagcgg ataaattgct ggctaaactt aacctgcgct ttaaaagcga
       61 caagttagtg ggcgatcttt ccatcggtga ccagcaaatg gttgaaatcg ccaaagtgct
      121 gagctttgag tcgaaagtca tcattatgga tgaaccgacc gatgcgctga ccgataccga
      181 aaccgaatcc ctgttccgcg tcatccgcga gctgaaatcg caaggccact gtattgtcta
      241 tatctcccac cgcatgaaag aaatcttcga gatttgcgat gacgttaccg tttttcgtga
      301 tgggcaattt attgctgagc gcgaagtggc atcactgacc gaagattcgc tgattgagat
      361 gatggtgggc cgcaagctgg aagatcaata tccacacctg gacaaagcgc caggagatat
      421 ccgcctgaaa gtcgataatc tctgcggacc tggcgttaac gatgtctctt ttactttacg
      481 caaaggcgaa attcttggcg tctctggttt gatgggcgca ggtcgtaccg aactgatgaa
      541 agtgctctac ggcgcactgc cgcgcaccag cggttacgtc accctggatg ggcatgaagt
      601 cgttacccgt tcaccgcagg atggcctggc aaacggcatt gtgtatatct ccgaagaccg
      661 taaacgtgac ggtttagtgc tgggcatgtc agtaaaagag aacatgtcgc tgacagcgct
      721 gcgctacttc agccgcgctg gcggcagttt gaagcatgcc gatgaacagc aggctgtgag
      781 tgatttcatt cgtctgttta atgtgaaaac gccgtcgatg gaacaggcaa ttggtctgct
      841 ttccggtggc aatcagcaaa aagtggcgat tgcccgcggt ctgatgacac ccccaaagta
      901 ttgatccttg atgagcctac ccgtggcgta gatgtcggcg cgaaaaaaga gatctatcaa
      961 ctgattaacc agttcaaagc cgatggcttg agtatcattc tggtgtcatc ggagatgcca
     1021 gaagtattag gcatgagcga tcgcatcatc gtcatgcatg aagggcatct cagcggggaa
     1081 tttactcgtg agcaggccac ccaggaagtg ttaatggctg tcgctgtggg caagcttaat
     1141 cgcgtgaatc aggagtaaaa aaatgataac ccagactgtc tctggtcgcc gttatttcac
     1201 gaaagcgtgg ctgatggagc agaaatcgct tatcgctctg ctggtgctga tcgcgattgt
     1261 ctcgacgtta agcccgaact ttttcaccat caataactta ttcaatattc tccagcaaac
     1321 ttcggtgaac gccattatgg cggtcgggat gacgctggtg atcctgacgt cgggcataga
     1381 cttgtcggta ggttctctgt tggcgctaac cggcgcagtt gctgcatcta tcgtcggcat
     1441 tgaagtcaat gcgctggtgg ctgtcgctgc tgctctcacg ttaggtgccg caattggtgc
     1501 ggtaaccggg gtgattgtag cgaaaggtcg cgtccaggcg tttatcgcta cgctggttat
     1561 gatgctttta ctgcgcggcg tgaccatggt ttataccaac ggtagcccag tgaataccgg
     1621 ctttactgag aacgccgatc tgtttggctg gtttggtatt ggtcgtccgc tgggcgtacc
     1681 gacgccagtc tggatcatgg ggattgtctt cctcgcggcc tggtacatgc tgcatcacac
     1741 gcgtctgggg cgttacatct acgcgctggg cggcaacgaa gcggcaacgc gtctttctgg
     1801 tatcaacgtc aataaaatca aaatcatcgt ctattctctt tgtggtctgc tggcatcgct
     1861 ggccgggatc attgaagtgg cgcgtctctc ctctgcacaa cccacggcgg ggactggcta
     1921 tgagctggat gctattgctg cggtggttct gggcggtacg agtctggcgg gcggaaaagg
     1981 tcgcattgtt gggacgttga tcggcgcatt aattcttggc ttccttaata atggattgaa
     2041 tttgttaggt gtttcctcct attaccagat gatcgtcaaa gcggtggtga ttttgctggc
     2101 ggtgctggta gacaacaaaa agcagtaata acgactacag gacatcttga atatgaacat
     2161 gaaaaaactg gctaccctgg tttccgctgt tgcgctaagc gccaccgtca gtgcgaatgc
     2221 gatggcaaaa gacaccatcg cgctggtggt ctccacgctt aacaacccgt tctttgtatc
     2281 gctgaaagat ggcgcgcaga aagaggcgga taaacttggc tataacctgg tggtgctgga
     2341 ctcccagaac aacccggcga aagagctggc gaacgtgcag gacttaaccg ttcgcggcac
     2401 caaaattctg ctgattaacc cgaccgactc cgacgcagtg ggtaatgctg tgaagatggc
     2461 taactaggcg aacattccgg ttatcactct tgaccgccag gcaacgaaag gtgaagtggt
     2521 gagccacatt gcatctgata acgtactggg cggcaaaatc gccggtgatt acatcgcgaa
     2581 gaaagcgggt gaaggtgcca aagttatcga gctgcaaggc attgccggta catccgcagc
     2641 ccgtgaacgt ggcgaaggct tccagctggc cgttgctgct cacaagttta atgttcttgc
     2701 cagccagcca gcagattttg atcgcaccaa aggtttgaac gtaatgcaga acctgttgac
     2761 cgctcatccg gatgttcagg ctgtattcgc gcagaatgat gaaatggcgc tgggcgcgct
     2821 gcgcgcactg caaactgccg gtaaatcgga tgtgatggtc gtcggatttg acggtacacc
     2881 ggatggcgaa aaagcggtga atgatggcaa actagcagcg actatcgctc agctacccga
     2941 tcagattggc gcgaaaggcg ttgaaaccgc agataaagtg ctgaaaggcg agaaagttca
     3001 ggctaagtat ccggttgatc tgaaactggt tgttaagcag tagttttaat caggttgtat
     3061 gacctgatgg tgacataaat acgtcatcga cagatgaacg tgtaatataa agaaaagcag
     3121 ggcacgcgcc accctaacac ggtggcgcat tttatggaca tcccgaatat gcaaaacgca
     3181 ggcagcctcg ttgttcttgg cagcattaat gctgaccaca ttcttaatct tcaatctttt
     3241 cctactccag gcgaaaccgt aaccggtaac cactatcagg ttgcatttgg cggcaaaggc
     3301 gcgaatcagg ctgtggctgc tgggcgtagc ggtgcgaata tcgcgtttat tgcctgtact
     3361 ggcgatgaca gcattggcga gagcgttcgc cagcagctcg ccactgataa catcgatatt
     3421 actccggtca gcgtgatcaa aggcgaatca acaggtgtgg cgctgatttt tgttaatggc
     3481 gaaggtgaga atgtcatcgg tattcatgcc ggcgctaatg ctgctctttc cccatcgctg
     3541 gtggaagcgc aacgtgagcg tattgccaac gcgtcagcgt tattaatgca gctggaatca
     3601 ccactcgaaa gtgtgatggc agcggcgaaa atcgcccatc aaaataagac tatcgttgcg
     3661 cttaacccgg ctccggctcg cgaacttcct gacgaactgc tggcgctggt ggacattatt
     3721 acgccaaacg aaacggaagc agaaaagctc accggtattc gtgttgaaaa tgatgaagat
     3781 gcagcgaagg cggcgcaggt actgcatgaa aagggtatcc gtactgtact gattacttta
     3841 ggaagtcgtg gtgtatgggc tagcgtgaat ggtgaaggtc agcgcgttcc tggattccgg
     3901 gtgcaggctg tcgataccat tgctgccgga gataccttta acggcgcgtt aatcacggcg
     3961 ttgctggaag aaaaaccatt gccagaggcg attcggtttg cccatgctgc cgctgcgatt
     4021 gccgtaacac gtaaaggcgc acaaccttcc gtaccgtggc gtgaagagat cgacgcattt
     4081 ttagacaggc agaggtgacg cttggctaca atgaaagatg ttgcccgttt ggcgggcgtt
     4141 tctacctcaa cagtttctca cgttatcaat aaagatcgct tcgtcagtga agcgattacc
     4201 gccaaagttg aagcggcgat taaagaactc aattacgcgc catcagctct ggcgcgtagc
     4261 ctcaaactca atcaaacaca taccattggc atgttgatca ctgccagtac caatcctttc
     4321 tattcagaac tggtgcgtgg cgttgaacgc agctgcttcg aacgcggtta tagtctcgtc
     4381 ctttgcaata ccgaaggcga tgaacagcgg atgaatcgca atctggaaac gctgatgcaa
     4441 aaacgcgttg atggcttact gttactgtgt accgaaacgc atcagccttc gcgtgaaatc
     4501 atgcaacgtt atccgacagt gcctaccgtg atgatggact gggctccgtt cgatggcgac
     4561 agcgatctta ttcaggataa ctcgttgctg ggcggagact tagcaacgca atatctgatc
     4621 gataaaggtc atacccgtat cgcctgtatt accggtccgc tggataaaac tccggcgcgc
     4681 ctgcggttgg aaggttatcg ggcggcgatg aaacgtgcgg gtctcaacat tcctgatggc
     4741 tatgaagtca ctggtgattt tgaatttaac ggcgggtttg acgccatgcg ccaactgtta
     4801 tcacatccgc tgcgtcctca ggccgtcttt accggaaatg acgctatggc tgttggcgtt
     4861 taccaggcgt tatatcaggc agagttacag gttccgcagg atatcgcggt gattggctat
     4921 gacgatatcg aactggcaag ctttatgacg ccaccattaa ccactatcca ccaaccgaaa
     4981 gatgaactgg gggagctggc gattgatgta ctcatccatc ggataaccca gccgaccctt
     5041 cagcaacaac gattacaact tactccgatt ctgatggaac gcggttcggc atagatttac
     5101 gctgtctttt gatcaaatta ttaccatcgg ttgttttcag aagcatgaac attgctgctg
     5161 aagcaacagt aataatgccc atcgtgataa acgtatagtg gaattgttcg acagtcgttg
     5221 tgccttccat cccttcataa atgcgaagga cggccgcact tacagcaacg cctaaactaa
     5281 tcgacagttg ctgcgtgacc gccagaacac tgttaccgct gctggcgttg tcatcggtca
     5341 gatcggcaag tgtgatggta ttcatcgcgg taaactgcgt cgacatagcc atccctaata
     5401 taaacaacgg caagatcagc atccatatcg ccattgccgg tgattgcaaa gagaactgag
     5461 cgatcattag cccaataatc accgtgatcc ccactaatgt atggcgatag cccagacgac
     5521 gtaagacttg ggtaaccatc gattttgcaa taatggaacc taacgctgtc ggtgccatca
     5581 tacagccggc aataaacgcc tgataaccaa atcctacctg taacatcaat ggcataagga
     5641 acggtacaca gccggtcccc agacgggttg caatattgcc tacgataccg atcgagaaag
     5701 tgcgggtttt aaataaatct aatgaaatta atgggtttgg cgtacgtcgc gcatggagaa
     5761 tatagagaag cagtaacccg atgctggtga caattaccgt caaggcaatc cagctggcga
     5821 caatcttttc cccgaatagc tctattccgc ttgaaaagag aacaaggctg aggccaaaca
     5881 gcaaaaagcc agtgatatcg aatctgcgtc gtgcggtggt gaaattgggc atatgtttgc
     5941 gcgcgtaaag aaggcccgca atacctatgg ggatattaat taaaaatatc cagtgccagg
     6001 ttgcccaggt gaccagcacg ccgccaagaa cggggcctaa aattggcccc accagacccg
     6061 gcatggcgac aaaattcaat accggaagaa gttcattacg aggataagca cgcagtaagg
     6121 ccggccgagc aacaggcatc atcattgcgt cgcctatccc ttgaataacc cggaagacaa
     6181 ccagctgtgg tagcgaatta gaaagtgcgc aggccagaga acccaacgtg aacagactca
     6241 cggcaagggt aaaaatgcga cgcgtaccga agcgatcggc tagccatccg cttaccggaa
     6301 taagcatcgc caccgtcagc gtataactga tgatggctga ttgcatcgcg agaggagaac
     6361 gattaaggct atgagcgatt gcgggtaagg cggtattcag aatagtggca tcaagtgcct
     6421 gcatgaagaa ggccatcgcc gcgatccacg gcaaacccga catactacgc ttctttttat
     6481 cgctcattca atgtcctgtt atcgggttat cacttatcag gtgagcgtag cagcgcctga
     6541 caagctttaa atgccgcgtc gccatcgctt tggataatcg catcgacaat cgcctggtgc
     6601 agatccagct ttatcactgt gtcgctggta attgacgtga agtaagtgtg ataaaccgaa
     6661 tggaatagcg aggcgaatga ggtcaaaaac ggattggcgc tcatttcata gatatgctca
     6721 tgccaggcca tatcgacttc gatccagcgt tcacggcgaa agttctcttt taatgccgcc
     6781 atttcggcca ttaacgtatt gagatgcgcc ttctgttccg cggtgccaac cgttgctgcc
     6841 agtaggcagg cttgcggctc cagacagata cgcataacca gaaagtgatc gatgacctga
     6901 tgaaagttct cttctgttat ccaccaagta agcaattcct gatcaagaaa attccagttc
     6961 gattgtggca tgacccgagt accaattcgc ggtcgcggta aaaccatccc ttttgccgtt
     7021 aacgttttga ccgcttcgcg taccgctgta cgactcactc caaattgctc gcccagctca
     7081 atctcaccgg gcaaaatggt gccgggttca tattcacctt ttaagatccg ttgcgccagc
     7141 ttctcagcca gaacatacga aaggtttttc tgtgcagcta actgttgtgc gcttaaaggc
     7201 attacttatc ttcctttttc tttttattcc tccttagtat gccaccagga agtgtgatta
     7261 cggttgcaaa aacggcaaat tgcttgtttt atggcacatt aacggggtgt tttgctgaaa
     7321 aaatgcgcgg tcagaaaatt attttaaatt tcctcttgtc aggccgggat aactccctat
     7381 aatacgccac cactgacacg gaacaacggc aaatacgccg ccgggtcagc ggggttctcc
     7441 tgagaattcc gacagaaaaa gcaaaaataa atgattgact ctgtagcggg aaagcgtatt
     7501 atgcaccccc cccgcgccgc tgagaaaaag cgaagcgcac tgctctttaa caatttatca
     7561 gacaatctgt gtgggcactc gaagatacgg attcttaacg tcgcaagacg aaaaatgaat
     7621 accaagtctc aagagtgaac acgtaattca ttacgaagtt taattctttg agc
//
LOCUS       DAEBXN010000164         7600 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.164, whole genome shotgun sequence.
ACCESSION   DAEBXN010000164 DAEBXN010000000
VERSION     DAEBXN010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7600
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.164"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(123..1750)
                     /gene="yeeO"
                     /locus_tag="KJL99_003890"
                     /pseudo
     CDS             complement(123..1750)
                     /gene="yeeO"
                     /locus_tag="KJL99_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
     gene            1755..1830
                     /locus_tag="KJL99_003891"
     tRNA            1755..1830
                     /locus_tag="KJL99_003891"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1788..1790,aa:Asn,seq:gtt)
     gene            complement(1868..2818)
                     /gene="cbl"
                     /locus_tag="KJL99_003892"
     CDS             complement(1868..2818)
                     /gene="cbl"
                     /locus_tag="KJL99_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HBD4438451.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEF
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGSPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFCWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRTDDAFTRKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(2920..3837)
                     /gene="nac"
                     /locus_tag="KJL99_003893"
     CDS             complement(2920..3837)
                     /gene="nac"
                     /locus_tag="KJL99_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HBD4438452.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            4165..4240
                     /locus_tag="KJL99_003894"
     tRNA            4165..4240
                     /locus_tag="KJL99_003894"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4198..4200,aa:Asn,seq:gtt)
     gene            complement(4296..5231)
                     /gene="ldtA"
                     /locus_tag="KJL99_003895"
     CDS             complement(4296..5231)
                     /gene="ldtA"
                     /locus_tag="KJL99_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HBD4438453.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNIRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(5293..6372)
                     /gene="cobT"
                     /locus_tag="KJL99_003896"
     CDS             complement(5293..6372)
                     /gene="cobT"
                     /locus_tag="KJL99_003896"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HBD4438454.1"
                     /translation="MQIIADLLNTIPAINSAAMSRAQRHVDGLFKPVGSLGKLEALAI
                     QLAGMPGVNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(6384..7127)
                     /gene="cobS"
                     /locus_tag="KJL99_003897"
     CDS             complement(6384..7127)
                     /gene="cobS"
                     /locus_tag="KJL99_003897"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HBD4438455.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLALMTGEFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLLGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(7124..>7600)
                     /gene="cobU"
                     /locus_tag="KJL99_003898"
     CDS             complement(7124..>7600)
                     /gene="cobU"
                     /locus_tag="KJL99_003898"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HBD4438456.1"
                     /translation="SSQVLYIATSQILDDEMAARIEHHRQSRPEHWRTVERWQHLDEL
                     IHADINPNEAVLLECVTTMVTNLLFDYGGDKDPDEWDYQAMEQAINAEIQSLIAACQR
                     CPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQQLAAAANEVWLVVSGIGVKIK"
BASE COUNT         2099 a         2056 c         1648 g         1797 t
ORIGIN      
        1 ctaccgactg aactacagag gaatcgtgag aacgaggcga atattagcga tgcccaccca
       61 caatgtcaaa gcctgttttt taaatttgaa atcgtttgct gaaataatct gcattttgtc
      121 gtttattccg acacaactgg ctttttttca cacttttgcg gctcggatcg agggtatttc
      181 cacagccaac gtccagtaac cattcgccag taaaacagca cggcccgcac agcccagtcg
      241 gcaaacattc ccatccagac accaaccaca ccccagccaa gcatgattcc ctgcacataa
      301 ccgactacaa cccggcaacc ccacatgctc aacatagaaa cccacatggc gtaacgggca
      361 tcacgagcac ccttaaatcc agcgggtagc acccatgagg cggaccaaat aggcataaat
      421 aaagcattta gctaaatcag aatcacaacg acgtgtttaa cctgtggatc ctgggtgtaa
      481 aacgatgcca taaccccggc aaagggagcc gttagccagg cgatggccgt taatccaaga
      541 gtggacagcc agaacacatg ccgcaactga atctctgctt gcgctatctg ccctaccccc
      601 aaccttcggc ctgtaatgat cgtagaagca gaaccgagcg cacttccggg taagttgata
      661 agagccgcaa ttgagaacgc gataaaattt ccggcaataa cgctggtccc catcccggca
      721 acgaacattt gggttaataa ccgaccactg gtaaataaca ctgattcgac actcgctgga
      781 ataccaatcc ccatgacttc ccagataatg ctaaaattca gcggtttaaa atagctcttt
      841 aacgaaatcc ttagcgcagg attaaaacca atcgccagca cccacaaaat tgcaactgcg
      901 ccaatataac gagaaatggt taaacccaac ccagccccga ccaggagaaa aggccgtaaa
      961 tcaatatgcc gctaataata atattaagaa tattcaggct accgttaatc agtagcggta
     1021 ttttcgtatt ccctgcacca cgaagtgccc cgctaccaat aagagtgatg gcagcggctg
     1081 gataactgag tacggtcagc tccagataag tcaacgccag tgctttaact tctgtcgtgg
     1141 catcacccgc gacgaaatca ataatttgtt cgccaaaatg atgaattagc gttgccaaca
     1201 gtacggcaaa caacgtcatg atcaccaatg actgccgcgt cgccaccctc gctcgtcgtc
     1261 gatcccgctt accgagacta aatgctacaa cgacagtagt accaagatcg atagctgcaa
     1321 aaaaagccat aatgaccata ttgaagctgt ccgccaaccc cacgccggcc atcgcatctt
     1381 ttcccagcca actgaccaga aaagtgctca ggacccccat caacaggaca caggcgttct
     1441 ccatgaagat aggaacagca agcggggtta tctcgcgcta gaacaacact ttgtagctct
     1501 tgcgtttaga gtgccagcga gtgccgtgaa caacctggcg taaagcagag gagatattca
     1561 aagccgacct taattgcaga aagtgaaacc acatttcaaa tgatgaggga gaatcagcaa
     1621 agctgcaaag attttcgcca acaaattgtc tgcaaatgca acaaactgtt gatagaaacg
     1681 gcaaacagtt gggaaattta aaaatcgggt ttgacaaaat atttttcgcc gttaagatgt
     1741 gcctcaacag cgattcctct gtagttcagt cggtagaacg gcggactgtt aatccgtatg
     1801 tcactggttc gagtccagtc agaggagcca aatttctgaa aagcccgctt ttatagcggg
     1861 ctttttgcta tatctgataa tcaatttcct cttcactgtt ttccatcacc tggcgcttga
     1921 tatcctcaac tgacagtcct gcattacaaa gttccagaaa gcgccaaaca tagttacgct
     1981 gaagttgtcc tcgcttcagt cccaaccaga cagtattagc atcgaaaaga tgccgcgtat
     2041 ccaggcggat taaattctct tcctcttgtt cgccgctgga ttgttcggca actaatccga
     2101 tcccaagccc aagagcaaca taggttttaa tgacatcaga atcctgtgca cttaatacaa
     2161 tatctgccgg caaacctttg cgggtaaatg cgtcatcagt gcgtgagcgc cccgtaattc
     2221 cctgtcggta agtaattaac ggccacttcg ctattgattc cagcgtcaat ggtgtaattt
     2281 gcgttaaggg atgatcgagt ggaacaagca aactatggtg ccaacaaaac catgggaagg
     2341 cgacgagctg cgggtcatta ctcaaacgct cgctggcgat accaatatca gcttcgccat
     2401 tttgcaacaa cgtcgcaatt tcctgtggcg aaccctggat tagctcgagc cgaacctccg
     2461 ggaaaagttc gcgaaaagct ttaatgacct ctggcaagct ataacgtgcc tgagtatgcg
     2521 tcgttgcaat agtgagaaca ccagacgtat cgttggtaaa caggtctgca agccgacgaa
     2581 cattactggc ttcattcaga atacgttctg caatgaccag taatgctttg cccggttcag
     2641 tcatccccag cagtcgctta cctcgtcgaa caaatatttc gatgccaaat tcatcctcca
     2701 gttcccgaat atgacggcta acgcctgact gtgaggtaaa aagcatattc gcaacctctg
     2761 tcaggttgta atcctgacgt gcagcctcgc ggattatctt tagttgttgg aaattcacgg
     2821 taaactccgg gcagttcaga tatcccgtta ttgttaaagt ctaatgcccg gcataacaaa
     2881 taataaaaac ccgcatctta ttccatcccg atataacgct tagctcacca attgccactg
     2941 ccttttttcc atcactggag aactaatcac tgacattaac aactctttca ctgcctgtgc
     3001 ctgtggtgac aagtttgctc tggcgggtaa atttaaggac aaagagagac tcattgaagg
     3061 agtggtaatg cgtgacatcc acccatttac tgcgccacat aacgaacgcg cagccgattc
     3121 aggtaatact gcaacgccca tgccgctggc aatcgctgcg gtaagcgtgg caatagactc
     3181 aatttcacca atgacttttg ccgtgagccg ccgtagggaa aaagcctcat caacacgaag
     3241 tctaacagcg ctgtaatcac tggggagaaa aaggttcatt tgcgcaatag cattaacatc
     3301 aacgttttgc cccgggcaat cttgagttcc taccaggaaa agatcttctt tcagcaaagc
     3361 ctgactggat accccagcca caggggaatg ctcataaatc accgccatat cgagttggtg
     3421 atttatcagt ttttcgttaa gcactgcacc actattttca tgaagataga taacgacctc
     3481 cgggaattca gcgcgaaccg cctgtaataa gggcatggtg atggatgacg cagcggttcc
     3541 tggcgcaaag ccaatcgaga cttgccccga taatgactga ccaacgttat gcaccgccag
     3601 ttgggcctgc tcacactgac gtaaaatggc ccgtgcatgg gtatagagaa tttttccggc
     3661 gtctgttggc gtaacgcccc gttttgtgcg aatcaaaagc tgttgattta actcaccttc
     3721 cagtatggca acctgttggc tgagcgctgg ttgtgcgata tgcaatactt cagcagcctg
     3781 ggtcaggcta ccaatatcta caatttttac gaagtatttc aggcgtctga agttcatgtt
     3841 gcctccggtt tttaagaatc ggcccaagtg ccgccattac ttacaaccag tatgcaagat
     3901 gcctgccagt tctattttgg cgttgatgcg caagctaacc aactgtcaaa taagagatta
     3961 tgatagattc gtcattggcc ctttttatca gcagtcgcat tcacctgccc tataaaagga
     4021 gggtatgcac cacgatggtt cattacccaa taagattgaa agctcaccac tttgttgaaa
     4081 ttggcagcaa acaaacaaaa aaaatgcatt tcaccctttg acatcaccat gcactgccat
     4141 taatatgcgc cccgttcaca cgattcctct gtagttcagt cggtagaacg gcggactgtt
     4201 aatccgtatg tcactggttc gagtccagtc agaggagcca aattcaaaaa agcctgcttt
     4261 cgagcaggct ttttactttc taattaccaa cgctcttaaa acatctgtct tgaaccagaa
     4321 ctaatttgca caggcattcc cgatcgatgt tgcaacgcag catttgcgcg atttacatca
     4381 acttcttgcc cgttgataaa cgcccgcaaa gatggggtta ccggcaatgg cacttttcgg
     4441 tcagactcat attctgcacg attgcgcgac agcggctcat gaacttccag ccactttgaa
     4501 ccatccggtt ccgttgtgta tttcactggc tgatcaataa tttgtacacg agtgcctaca
     4561 ggaacattat caaacagata tttgatatcg tcattacgca gacgaataca gccctgactt
     4621 acacggagcc caataccaaa attggcattg gtaccgtgga tggcatacaa cctgccaata
     4681 taaatcgcgt acagccccat ggggttatcg ggccccgcag gaacaaatgc gggcaaactc
     4741 tcccctcgtt tcgcatattc gcgccggatg ttcggcgttg gcgtccaggt tggtgcttct
     4801 tgtttacgtt caacggtagt cacccagtta cgcggggttt ctcgcccagc ctggccgata
     4861 ccaataggaa agacttccac tgtattactg tctggcggat agtaataaag acgcatttca
     4921 gcgacgttaa ccacaatccc tttacgaaca gtggcgggca aaatcagttg ctgcggaatg
     4981 gtgagttgtg agccaggctt cggcaaaaaa acatcagcgc ccggattcgc ttccagcata
     5041 ttacttaacc cttgcccgta ttgtgcggca aaagtctcca gcggctgagt attgtgatca
     5101 ggaacagtta cagtaagcga ctgccccact aaacggctac cctctggagg taatggataa
     5161 gttaccgcca ggctattttg gctggcaaaa agcagagcaa atgagcaaag aatatttaca
     5221 cgacgcatca tgtccctttc ctaagtcgcg aatactatcc gataagtata gcttttatca
     5281 gacttttcgt ttttagctgt tcaaatcaga agtcgtattc cccggtagaa caatattact
     5341 ggcagcaagt tcgcccatgt tgttgtatat cgcacaagca gcttcgatga tggacatcgc
     5401 cagagctgca ccactcccct cacctaaacg catatccata ttgagataag gctccagccc
     5461 caaatgcgag agcgctatac gcgcgccttt ttctgccgac aagtgagaag gaatgagata
     5521 cggtttgatt gcaggagaca tctggcaggc tgcgagcgca gcagcataag aaagaaatcc
     5581 atccagcaac acgggtaaac cgcaggaagc agcacctaac atcactccag ctattccgac
     5641 caaatcaaat ccacccactt ttgccaggac atcaacacca tcctgaggat ttggttgatt
     5701 caacgtaatc gcccgacgca caacatcaat tttattagcc agtttatctg tcggcaggtt
     5761 tgcgccaatc ccaaccactt cttcaggatc ccggccagtg attgtgctga ctattgccgc
     5821 tgccggtgtc gtgtttgcca tccccagttc acctacacca aacagcgtga caccgttttt
     5881 cgccagctcc cgcgtataac atatgacgtc caaaagcaac ttttcagcct gacggcgact
     5941 cattgccgga gctgaagcaa tattgccgct acctcgtgcg acacgcatgt tgataagccc
     6001 ggggataggc tcagcagtat caataccaac atcaattacg tggacgttag cgcccgcttg
     6061 tgctgccagc acacacacgc cggttgttcc acgggtcata ttttcagcct gtatggctgt
     6121 cacttctttt ggagaaatag cgaccccttc ctcccagacg ccgtgatcgg cacacataac
     6181 cagtaccgct tttttgccca catgcggtat gccattcacc cccggcattc ctgccagttg
     6241 tatggcaagc gcctccagct ttcctaggct accaacaggt ttgaataacc cgtcaacatg
     6301 ccgttgtgca cgcgacatag cggcagaatt gatggctggg atcgtattca gtaaatcggc
     6361 aattatttgc atgtcacgtt tctttatagc agtgccagca ggaagaccag ttcaccaagc
     6421 tctatggctg ctcccagcgt atcgcccgtt tgcccgccca gcgtacgttt gagaagctga
     6481 ccgaggatga aaatcgccac catcgtcacc accatagcag ccacaccatg catacccagc
     6541 aataatacgg cagcaaaaat agcggctaag ccgagtgtga cgcaggtttg tcgcccatca
     6601 attttgccaa tgaacacatt gccaagccct tcttcccgcg cataacgatg acgatacatc
     6661 aataatgcgg cagtcccgcg gctgaccgca catgctgccg ccagcgatgc aaggatcggt
     6721 tcgccacgca gagccaactc gcttaacacc agaatctttg ccagtaccac aaaaatcaat
     6781 gccagaccgc cgtgggtgcc taaacggcta tcacgcatga tttccaacat gcgatcgcga
     6841 ctacgtgcag aaaatacgcc atcacaggta tcagcgaggc cgtccagatg gaattccccg
     6901 gtcatcagcg ccagcacaag tacgctaaac agtgccgcca gtggtgcgcc acaccatgcc
     6961 tgcagcacca tgaagaccag cccgctaatc gcgccaagca ataatccaat caaaggaaaa
     7021 gtaataatac cgcgagaata atgttcgaaa tccagtccct gggaccagcg acgcggtaca
     7081 ggcagacgcg taataaatga gagcatcgcc caaaataatt tgctcatttg atttttactc
     7141 caatacccga aaccaccagc catacttcat ttgccgcagc ggccaactgc tgatttaccc
     7201 gcccggcaat atcacgaaaa tgtcgtgcca gacgactctc cggcacaatc cccattccca
     7261 cttcgttagt cactaataca acctttgcgg ggcaacgttg gcaggcagca atcaacgact
     7321 gaatctcagc attaatcgcc tgttccatcg cctgataatc ccattcatca gggtctttat
     7381 cgccgccata atcaaacaac agattagtca ccattgtggt aacgcattcc agcaacacag
     7441 cctcattcgg gttaatgtct gcatgaatta attcatcaag atgttgccag cgctccactg
     7501 tgcgccagtg ctccgggcgg ctttgccgat gatgttctat ccgtgcagcc atctcatcat
     7561 caaggatttg cgaggtagcg atatacagaa cctgtgaaga
//
LOCUS       DAEBXN010000165         7536 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.165, whole genome shotgun sequence.
ACCESSION   DAEBXN010000165 DAEBXN010000000
VERSION     DAEBXN010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7536
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.165"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(300..1700)
                     /gene="asnS"
                     /locus_tag="KJL99_003899"
     CDS             complement(300..1700)
                     /gene="asnS"
                     /locus_tag="KJL99_003899"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HBD4438457.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(1869..3071)
                     /gene="pncB"
                     /locus_tag="KJL99_003900"
     CDS             complement(1869..3071)
                     /gene="pncB"
                     /locus_tag="KJL99_003900"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HBD4438458.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            3337..5952
                     /gene="pepN"
                     /locus_tag="KJL99_003901"
     CDS             3337..5952
                     /gene="pepN"
                     /locus_tag="KJL99_003901"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HBD4438459.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEEGALVISNLPERFTLKIINEISLA
                     ANTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNG
                     NRVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDR
                     GNLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKY
                     VLARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSD
                     LGSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMI
                     HTLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVT
                     VKDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVN
                     SVLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNGFSRW
                     DAAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVN
                     EMAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLR
                     NACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKW
                     HQDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAF
                     HAEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLE
                     NLSGDLYEKITKALA"
     gene            complement(6159..6926)
                     /gene="ssuB"
                     /locus_tag="KJL99_003902"
     CDS             complement(6159..6926)
                     /gene="ssuB"
                     /locus_tag="KJL99_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HBD4438460.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLHLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAAGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKISLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(6923..>7536)
                     /gene="ssuC"
                     /locus_tag="KJL99_003903"
     CDS             complement(6923..>7536)
                     /gene="ssuC"
                     /locus_tag="KJL99_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HBD4438461.1"
                     /translation="HLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQML
                     RNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARSYG
                     LSGIPLFIHVILPDALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREFLQ
                     TDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATI"
BASE COUNT         1918 a         2000 c         1908 g         1710 t
ORIGIN      
        1 taaattaact aaacggaaat tttgtttctg atggaaactt tatcgacctg gcacaaaatc
       61 ctctttcaga catccagaat gccaaaaaat agtatgaaaa ttgtgctatc agctaagaaa
      121 aaactaatcc gcaataagag tacgggttat tttttgtgcg taaatgttgc aatgtaatat
      181 tccgtaacag gatgatcgtt atggcggggg tgcaaataga ttgcttgact taacaaacag
      241 ataacttgac agaaaggata aaaaagagcc agcaggcgct ggctctttga ggaagaaaat
      301 cagaagctgg cgttgcgcgg agtacgtggg aacggaatca catcacgcac gttttgcacg
      361 ccagttacgt aagcaatcag gcgttcaaaa ccaagaccga aacctgaatg cggaacagta
      421 ccgtagcgac gcagatcgcg ataccaccag taatcttctt tattcaggcc catttccagc
      481 atacgctcgt ccagaacgtc cagacgttct tcacgctggg agccaccaat gatctcaccg
      541 atgcccggag ccagaacgtc catagccgca acggttttac cgtcttcgtt gaggcgcata
      601 tagaacgctt taatatcttt cggatagttt ttaaccacta ccggtgcttt aaagtgttct
      661 tccgccagat aacgctcatg ctcagaagag agatcgacgc cccagtaaac cgggttttca
      721 aacttcctgc cgcagttttc gagaatggtc actgcgtcgg tgtaatccac ctgcgcgaaa
      781 tcggcttcaa tgaagcgttc cagacgtgaa acggcatctt tatctacgcg ttcagcgaag
      841 aatttcatgt cgtctgcgcg ttcttcgaga accgctttga agacatattt cagcatggct
      901 tcagccagac ccgcgatatc gttcaggtta gcaaacgcca cttccggctc cagcatccag
      961 aattccgcca ggtgacggct ggtgttggag ttttcagcac ggaaagtcgg gccgaaggta
     1021 taaattttgg ataatgcgca agcgtaggtt tcgccgttca actggccaga tacggtcagg
     1081 aaagactctt taccaaagaa gtctttgtcg aaatccactt tgccctgatc gttacgcggc
     1141 aggttttcca gatccagcgt agaaacgcgg aacatttcgc ctgcaccttc ggtatcagat
     1201 gcggtaatca gtggcgttga aacccagaag aatccctgct cgttaaagaa gcgatgcagc
     1261 gcctgcgcca gcgtatggcg aacgcgcgcg acggcaccaa tcaggtttgt gcgcggacgc
     1321 aggtgagcga cttcacgcag atactcaatg ctgtggcgtt ttgccgccat cgggtaagtg
     1381 tctggatctt caacccaacc agcaacttca accttgctgg cctgaatttc aaattgttgc
     1441 ccctggcccg gcgatgccac gactttaccc gtcacaatga ccgagcagcc ggtggtcaga
     1501 cgcaggacgt cttcattgta attgggcaga gaattattga tgacagcctg tacaggatca
     1561 aagcaggaac cgtcataaac ggcgaggaag gagatgccag cttttgaatc tcggcgggta
     1621 cgtacccatc cgcgtacggt gacttcgctg tcaacggcta cgcggccctg gagtacgtcg
     1681 gctacaggca caacgctcat aatattctct ctgttaatag tcggaaaaaa taaacacttg
     1741 tccacccgta aatggggggg atacctatgt tacctggcat ctgcaatcag acaagcagaa
     1801 ttcgcaaatg cagcgaaaga tttcggaatt aaaggtaaaa aagggagccg attagctccc
     1861 tgatgatatt aactggcttt tttaatatgc ggaaggtcga acgctttgcg cagcgcccga
     1921 acaaacgctt tatcatggca gatagttttg ccagggctgt cagaaagttt cgccaccggt
     1981 ttaccgttac actctaccaa cttaatcaca atattcaggg gttttacctg ggggatatcg
     2041 caggtcaggc gagtcccaat accaaaactt aattgcacgc gggaagagaa gtggcggtat
     2101 agctcaaccg ctttgcgtaa atccagattg tcagagaaaa ccagcgtttt actctgtgga
     2161 tcaattccca gcttttcata atgtgcaatg gctttttcac cccattcaac cgggtcgcca
     2221 gagtcatgac gcaggccctg ataccgacta gcgaactcga caccgaaatc acgcaggaaa
     2281 gcatccatag tgatgcagtc ggttaatgca atgccaagtt ggtcgggata ctcttccagc
     2341 caggcagcaa gtgcagctcg ctggctgttg gctagatccg ggctgatttg ctgatgtgcc
     2401 tggaaccatt cgtgtgcctg tgttcccatc ggcgtgaggg aaagccgacg cgccagatcg
     2461 tagttgctgg tgcccacgaa ccaggattcc tgttgcagac gcttaacgat ggtttcttgt
     2521 acttcgcgag aaaaacggcg acgggtgcca aaatccatca gatggaagcg cgacatatca
     2581 agaccggcgg ttaacgccga gaagtcgact aatttgcttt ccagcgtgtc gagggcttgc
     2641 gcaacgtcgg cctgcggtga gcgatagcga tgtaccattt cactgataac cgccagcaaa
     2701 ggaacttccc agaggatgac ttcacgccac ggaccgctta agcgaatatc cagcttgcca
     2761 ttatcgttgg acacggtgac ttgttccggg ttaaagcgga actcgcgtaa ccagttaaga
     2821 tagtcggcct taaagaaagg cagggcagag agccactgat attcatcatc ctgcaggcgc
     2881 aggtgctgca tcgcctgaat ctgttcacga atagcatcgg cataaatacc cagcagatcg
     2941 tcacctcggc aacgaaactc cgccgcgaca tgcacatcgt aatagtgatg aaacacggct
     3001 tgctgcatat gcaacttata agcatctgta tccagcaacg agtgcagaac aggagaagcg
     3061 aattgtgtca taggtgcgca gtagcgtcct cgtacaggag cgtttagtac aataaacatc
     3121 ttcaggaact gctggagtat accttgtttc gcaatttatt gaaccccgat cacaccatat
     3181 gccacctttc tggtcgatgg cattacgcgg tgtatgttat aaaaatgtag caataaaggc
     3241 gtttgtacct gaaaagatga agattctgca tagcgcgatt tacgcaacag gaatagactg
     3301 aacaccagac tctataaaag atgctaaagg ttatttatga ctcaacagcc acaagccaaa
     3361 taccgtcacg attatcgtgc gccggattac cagattactg atattgactt gacctttgac
     3421 ctcgacgcgc aaaagacggt cgttaccgcg gtcagccagg ctgtccgtca tggtgcatca
     3481 gatgctccgc ttcgtctcaa cggcgaagac ctcaaactgg tttctgttca tattaatgat
     3541 gagccgtgga ccgcctggaa agaagaagaa gagggcgcac tggtcatcag taatttgccg
     3601 gagcgtttta cgcttaagat cattaatgaa ataagcctgg cggcgaatac ggcgctggaa
     3661 gggctttatc agtcaggcga tgcgctttgc acccagtgtg aagccgaagg tttccgccat
     3721 attacgtatt atctcgaccg cccggacgtg ctggcgcgtt ttaccaccaa aattattgcc
     3781 gataaaatca aatatccctt cctgctttcc aatggtaacc gcgttgcgca aggcgaactc
     3841 gaaaacggac gccattgggt acagtggcag gacccgttcc cgaaaccgtg ctacctgttt
     3901 gcgctggtgg caggcgactt tgatgtactg cgcgatacct ttaccacgcg ttctggtcgc
     3961 gaagtagcac tggagctgta cgtcgatcgc ggcaaccttg atcgcgcgcc gtgggcgatg
     4021 acctcgctga aaaactccat gaaatgggat gaagaacgct ttggcctgga gtatgacctc
     4081 gacatctata tgatcgtcgc ggtggatttc ttcaatatgg gcgcaatgga gaataagggt
     4141 ctgaatatct ttaactccaa atatgtgctg gcccgcaccg acaccgccac cgacaaagat
     4201 tacctcgata ttgaacgcgt tatcggccat gaatatttcc ataactggac cggtaaccga
     4261 gtgacctgcc gcgactggtt ccagctcagc ctgaaagaag gtttaaccgt cttccgcgat
     4321 caggaattca gctctgacct tggttcccgc gcagttaacc gcatcaataa tgtgcgcacc
     4381 atgcgcggat tgcagtttgc ggaagacgcc agcccgatgg cgcacccaat ccgcccggac
     4441 atggtcattg agatgaacaa cttctacacc ctgaccgttt acgagaaggg cgcggaagtg
     4501 atccgtatga tccacaccct gctgggcgaa gaaaacttcc agaaagggat gcaactctat
     4561 ttcgagcgtc atgatggtag cgcagcgacc tgtgacgact ttgtgcaggc gatggaagat
     4621 gcgtcgaatg ttgatctctc ccatttccgc cgttggtaca gccagtccgg tacgccggtt
     4681 gtgaccgtca aagacgacta caatccggaa actgagcagt acaccctgac catcagccag
     4741 cgcacgccag ccacgccgga tcaggcagaa aaacagccgc tgcatattcc gtttgccatc
     4801 gaattgtatg acaacgaagg taaagtgatc ccgttgcaga aaggcggtca tccggtgaat
     4861 tccgtgctga acgtcaccca ggcggaacag acctttgttt ttgataatgt ctacttccag
     4921 ccggtgcctg cgctgctgtg cgaattctct gcgccagtga aactggaata taagtggagc
     4981 gatcagcaac tgaccttcct gatgcgtcat gcgcgtaatg gtttctcccg ttgggatgcg
     5041 gcgcaaagtt tgctggcaac ctacatcaag ctgaacgtcg cccgtcatca gcaagggcag
     5101 ccgctgtctc tgccggtaca tgtggctgac gctttccgcg cggtgctgct cgatgagaag
     5161 attgatccgg cgctggcggc agaaatcctg acgctgcctt ctgtcaatga aatggctgaa
     5221 ctgttcgata tcatagaccc gattgctatt gccgaagtac gcgaagcact cactcgtact
     5281 ctggcgactg aactggcgga tgagctgctg gctatttaca acgcgaatta ccagagcgag
     5341 taccgtgttg agcatgaaga tattgcaaaa cgcactctgc gtaatgcctg cctgcgcttc
     5401 cttgcttttg gtgaaacgca tctggctgac gtgctggtga gcaagcaata ccatgaagca
     5461 aacaatatga ccgatgcgct ggcagcgctt tctgcggcgg ttgctgcaca gctgccttgc
     5521 cgtgacgcgc tgatgcagga gtacgacgat aagtggcatc aggacggtct ggtgatggat
     5581 aaatggttta tcctgcaagc caccagcccg gcggcgaatg tgctggagac ggtgcgtggt
     5641 ctgttgcagc atcgctcatt taccatgagc aacccgaacc gtattcgttc gttgattggt
     5701 gcgtttgcgg gcagcaaccc ggcagcgttc catgccgaag atggcagcgg ttaccagttc
     5761 ctggtggaaa tgcttaccga cctcaacagc cgtaacccgc aggtagcttc acgtctgatt
     5821 gaaccgctga ttcgcctgaa acgttatgat gccaaacgtc aggagaaaat gcgcgcggcg
     5881 ctggaacagt tgaaagggct ggaaaatctc tctggcgatc tttacgagaa gataactaaa
     5941 gcactggctt gatacttttg agccaaaatg cctgatgcga cgcttgacgc gtcttatcag
     6001 gcctacaact ccgttgaaat tgctggtttt gtaggctgga taaggcgttc acgccgcatc
     6061 cggcagccgt gcccaactgc ctgatgcgat gcttgacgtg tcttatcagg cctacaagtc
     6121 cgttgaaaat gctggttttg tgcgccacat ccggcatatt aaccatgacg ccgaatcagt
     6181 tgttcagaat gcccacgccg cattacccgt tgcaatactt ccgcttccag ctccgctaat
     6241 cgcaccgacc ctaatcgacg cggacggggg atatccactg tcaaatccag actaattttt
     6301 ccctcttcaa ttaacaacac ccggtcagcc attgctaccg cttcgctcac atcatgcgtc
     6361 accaacagta cggtaaagcc gtgctgctgc caaagtgaca caatcaaatc ctgcatctcg
     6421 agtcgcgtta aggcgtccag tgccccgagc ggttcatcaa gcaacaataa acccggtcga
     6481 tgaatcaacg ctcttgccag cgccacacgc tgtttctgcc cgccagaaag tgcggcaggc
     6541 cattccccgg cgcgattctc cagtcctgcc gcagccaacg cccgacgcgc ggcatcgcgc
     6601 cactgacctt taaggcctaa cccaacgtta tcaatcaccg atttccacgg cagcagacgc
     6661 gcatcctgaa acatcattcg cgtatcatcc tgaatttcag ccagcggtgt ggtgcccgct
     6721 aacacatcgc ctgcggttgg cgtttccaga ccggccagca ggtgcagcag ggtactttta
     6781 ccaccaccgc tgcggcccac caccgccaca aactgacctg ccggaatatg taaatccagt
     6841 tggttcagga cgatattttc cgcgtaatgt ttgcttactg cattgagcaa caatggtgtg
     6901 ccctggttca gacgagcagt attcatatcg tggcctcctt caaatgataa gccgggttcc
     6961 agcgcaacca gaggcgctct aacagctgcg cgctgacgtc agcaagtttg ccgagcaggg
     7021 cgtaaagaat aatagcgact accaccacgt ctgtttgcaa aaactctcgc gcattcatcg
     7081 ccagataacc aatgccggaa ttggcagaaa tggtttcggc aacaatcagc gtcagccaca
     7141 tcaggcccaa cgcaaaacgc acgccgacca taattgaggg cagggcatcg ggcaggatca
     7201 catggataaa cagcggtatg ccggataatc catagctacg cgccatctcc accagcccgc
     7261 gatcgatatt acggatgcca tgccaggtgt tgatataaat ggggaacagc gtaccgagcg
     7321 ccaccagaaa gattttcgcg gattcatcaa tgccaaacca caaaatcacc agcgggatca
     7381 gcgccagatg cggcacgttg cgcaacatct gaattgaggt atccagcagc ctttctcccc
     7441 agcgcgacag tccgctaatc agtcccagaa tcaggcccag cgatccgcca attgaaaagc
     7501 caatcagcgc ccgccaggaa ctgatcgcca gatgct
//
LOCUS       DAEBXN010000166         7407 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.166, whole genome shotgun sequence.
ACCESSION   DAEBXN010000166 DAEBXN010000000
VERSION     DAEBXN010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7407
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.166"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..1013
                     /gene="ydfJ"
                     /locus_tag="KJL99_003904"
     CDS             <1..1013
                     /gene="ydfJ"
                     /locus_tag="KJL99_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HBD4438462.1"
                     /translation="LLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVAMGT
                     NCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVFEKV
                     NDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNKAIP
                     TDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYAPST
                     IMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPILAGI
                     FCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPRVVRAAQPSR
                     SL"
     gene            1102..2562
                     /locus_tag="KJL99_003905"
     CDS             1102..2562
                     /locus_tag="KJL99_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBD4438463.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLEHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYGMMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(2598..2801)
                     /gene="ydfZ"
                     /locus_tag="KJL99_003906"
     CDS             complement(2598..2801)
                     /gene="ydfZ"
                     /locus_tag="KJL99_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HBD4438464.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(2979..3665)
                     /gene="ydfH"
                     /locus_tag="KJL99_003907"
     CDS             complement(2979..3665)
                     /gene="ydfH"
                     /locus_tag="KJL99_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HBD4438465.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(3754..4500)
                     /gene="ydfG"
                     /locus_tag="KJL99_003908"
     CDS             complement(3754..4500)
                     /gene="ydfG"
                     /locus_tag="KJL99_003908"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HBD4438466.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELTDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            4637..6682
                     /gene="dcp"
                     /locus_tag="KJL99_003909"
     CDS             4637..6682
                     /gene="dcp"
                     /locus_tag="KJL99_003909"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HBD4438467.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELITRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(6727..7245)
                     /locus_tag="KJL99_003910"
     CDS             complement(6727..7245)
                     /locus_tag="KJL99_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HBD4438468.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
BASE COUNT         1834 a         1746 c         1884 g         1943 t
ORIGIN      
        1 tcttgctggt gacgctgcgt attattcagg gattgggtgc aggtgcggaa atttccggtg
       61 ccggtacgat gctggcggaa tatgcgccaa aaggtaagcg cggaattatc tcctcatttg
      121 tggctatggg aactaactgc ggcaccttaa gcgcaacggc aatctgggcc tttatgttct
      181 tcattctcag taaagaggaa ctgctggcgt ggggatggcg tataccgttc ctggcgagcg
      241 ttgtcgtgat ggtctttgct atctggttgc gtatgaatct gaaagaaagc ccggtctttg
      301 agaaggttaa cgacagcaac caaccgacag caaaacctgc acctgctggt agcatgttcc
      361 agagcaaatc cttctggctg gcaacagggc tgcgttttgg tcaggcgggt aactccggtt
      421 taattcagac tttccttgca ggctatttag tacagacgtt attgtttaac aaagcaattc
      481 caacagatgc attgatgatc agttcgattc tcggctttat gaccattccg ttccttggtt
      541 ggttatccga taaaattggt cgccggatcc cgtatattat tatgaatacc tctgcgattg
      601 tgctggcgtg gccaatgctt tctatcatcg tagataaaag ctatgccccg agcaccatta
      661 tggttgcact gattgtgatt cataactgtg cggtgctggg attatttgct ctggaaaaca
      721 ttaccatggc agaaatgttc ggctgtaaaa accgcttcac ccggatggct atttcaaaag
      781 aaattggtgg tcttatcgct tccggttttg gtcctatcct ggcgggtatt ttctgcacca
      841 tgacggaatc ctggtatccg atcgccatta tgatcatggc atattcagtg attggtttaa
      901 tctctgcgct gaaaatgcca gaggtgaaag accgtgattt aagtgcgctg gaagacgctg
      961 cggaagatca accgcgtgtt gtaagagctg cgcaaccttc cagaagtctg taaaccctca
     1021 atcccttctc ttaccggaga ggggattttt attcatataa caaaacatat agcttgccat
     1081 atttaaattt aaggaattgt tatgggaaat aatttgttat cagcaaaagc gacactccct
     1141 gtttatgatc gtaataacct ggccccaaga attgttcatt taggctttgg tgcatttcac
     1201 cgtgcgcatc agggcgtgta tgccgatatt cttgctacag aacatttcag tgactgggga
     1261 tattatgagg tcaacttaat cggcggcgaa cagcaaattg ccgatttaca acagcaagat
     1321 aatctttata ccgttgcgga aatgtcggcc gatgcgtgga cggctcgcgt cgttggcgtc
     1381 gttaaaaaag ccttgcacgt acagatggat ggcttagaaa ccgtgttggc tgcgatgtgt
     1441 gaaccgcaaa tcgcgattgt ctctctgaca atcaccgaaa aagggtattt ccactctccg
     1501 gcgaccggac agttaatgct cgagcacccg atggtcgctg ccgacgtgca aaatccccac
     1561 cagccgaaaa cagcaacagg ggtgattgtt gaggcgctgg ctcgccgtaa agcggcagga
     1621 cttcccgcat ttaccgtcat gtcatgtgac aacatgccag aaaacggtca tgttatgcgt
     1681 gacgttgtca cttcctacgc gcaagccgtt gatgtaaaac tggcacaatg gatcgaagat
     1741 aacgtgactt tcccatcaac aatggtggac cgtattgtgc ccgcagtgac agaggatacg
     1801 ctggcgaaaa tcgaacaact taccggtgtg cgcgatcctg ctggcgttgc ctgtgaacct
     1861 ttccgccagt gggttatcga agataacttt gtagccggac gtccggaatg ggaaaaagcg
     1921 ggagccgaac tggttagtga tgtgctgcct tatgaagaga tgaagttgcg catgctcaac
     1981 ggcagtcatt cattcctggc atatctgggt tatcttgctg gatatcagca cattaatgac
     2041 tgtatggaag atgaacatta tcgtcatgcg gcgtatggca tgatgttgca ggaacaagcg
     2101 ccgacgctga aagtgcaggg cgttgatttg caagattacg ctaaccgatt aattgcacgc
     2161 tatagcaacc cggcgttacg tcatcgaacc tggcagattg cgatggatgg cagccagaaa
     2221 ttgccacagc ggatgttgga ttctgttcgc tggcatctgg cgcatgacag caagttcgat
     2281 ctgctggcgc tgggcgtcgc gggttggatg cgttatgtcg gtggtgttga tgaacaggga
     2341 aatccgatag aaatcagtga cccactgtta cctgttattc agaaggctgt acaaagtagt
     2401 gccgaaggga aagcgcgcgt ccagtcattg ctggcgatta aggcaatctt tggtgatgat
     2461 ttgccagaca atagcttgtt tactgcaaaa gtgacggaag cgtacttgtc tttattagcg
     2521 catggcgcga aagcgaccgt ggcgaaatat tccgtgaagt aaaaaaagca atgccgccat
     2581 cggcggcatt cacacactta gttcatgccg agacgaatca ggtccagagg tgccagtttc
     2641 tcttcacaac cttcaacaac tacggttttt ccgcggcgga tttgctcagc ggtcagacct
     2701 tcagtatcaa tcgacaggat cttgccagtg tgaccggtgc cgctaaccat aacacggctg
     2761 ccagtggtga ttgcgttacg gttacgatcg taggtcgtca taggtgtttt ctcctttctg
     2821 atttacagtc gaagcccacg taacgggcgc aatataaata ctcctgtcga gtgaaatatt
     2881 ttttgttttt gatcaagctc acatcttttt tgatgcatgc atgagccgta aggcgggggg
     2941 aaaagcctct catcccggat gggatgagag taaatgaatt actcttcgct aaaccagtcg
     3001 ctgttttcct ggcggatctg gcgcacggat tcgctgattt cctgcaaatg ttgcgtcatt
     3061 gcacgttcta ccgcatcgcc atcacgtttt tgcagggcag agaaaatatc aagatgctgg
     3121 cgtaacagca tttctggtgg agaaacatgg tcgaaactca tatagcgcac gcgatcaacg
     3181 gtcgctttca ggttctcaat ggtatcccac gccagttgac agtcggcaat ctgcgtcagg
     3241 agttgatgga agttgtcatc aagttcaaaa aaatcatcca gttgcttgcg ctcaatggca
     3301 atgcgttgct ggtgaagatt ttgttccagt tggtagcact ggctttcggt aatcatgctc
     3361 gccgcccgac gcgccaccgc gcactcaatg gcctgacgga taaaactgcc gttgcgcacc
     3421 tgggccatgg aaattttgtt gacgtagctg ccacgctgcg gacgaatttg aatcaggccg
     3481 ttttccgcca gtttaataaa ggcttcacga accggctggc gtgacacatt gaaacgaaca
     3541 gaaacttctt tttccgacaa cggtgtgcct ggtgcaatca ggcaatggac aatgtcgcga
     3601 cgaagaatac gataaatctg ctgattgaca ggctgtgtgg gattaagttg cgtttcgacg
     3661 gtcattcgtt cttgcttgtg agtgagttaa ctgcatgagt ctaccacttt tttgtgacaa
     3721 gcaataaccc ggcagttacg ccgggtataa aaattactga cggtggacat tcagtccggc
     3781 atagctttgg gtaaccggca tcatttccag ggtattgata ttgacgtgag caggcagcgt
     3841 tgacacccac cagacggctt cgctgacatc ttctggcgtc aatgcaacgg tattttgata
     3901 ggttttttcc gctttaccgt catcgccttt aaagcggaca ttggaaaact cggtgccacc
     3961 caccagaccc ggttcgatgt cggtgacgcg caccgccgta ccatgcagat ccgtacgcag
     4021 attcaggcta aactgacgaa caaacgcttt cgtcgcaccg taaacgttac caccggcata
     4081 cggccagctg cctgccgttg agccaatgtt aataatatga ccatgattac gttcaaccat
     4141 acccggtaag acggcgcgcg tcatatatac taggcctttg ttgttggtat caatcatcgt
     4201 ttcccagtct tcaacgctgg ctttatgcgc aggctccatg cccaacgcca ggccggcatt
     4261 atttaccagg atatcaatat tgctccactc ggcagggagc gatgccagca tctcttcaat
     4321 agcggcgcgg ttgcgaacgt ccagttgggc gatatacaga ttatctccca gttcgtctgt
     4381 taactcctgc aaccgctcct ggcgacggcc agtggcgata actttatgcc cttgttgaat
     4441 aaaacgacga gtaatgcatt caccaaaacc tgccgttgct ccagttacta aaacgatcat
     4501 ctcactgttc ctcaacgctt ttgtgtgtca ataacatagc acgtgatgct taagagcggg
     4561 taatactggt ttgcaagatt gcactccgtt atcagtaagc ctacgctatg gaacacctcg
     4621 cgttcaggag aagaaaatga caacaatgaa tcctttcctt gtgcaaagca cactgccgta
     4681 tctggctcct cattttgatc aaattgccaa tcatcactat cgcccggcat tcgatgaggg
     4741 aatgcaacaa aagcgggcag aaattgctgc catcgcgctt aacccgcaaa cgcctgattt
     4801 caacaatact attctggcac tggaacaaag cggagaatta attacccgcg ttaccagcgt
     4861 cttttttgcg atgactgcgg cgcataccaa tgatgaatta cagcgtcttg atgaacagtt
     4921 ttccgctgaa ctggcggaac tggctaatga tatctatctg aacggtgaat tattcgcgcg
     4981 ggtagatgct gtctggcagc gccgtgaatc cctggggctt gatagtgaat ccatccgcct
     5041 ggtggaggtt attcatcaac gttttgtcct tgccggagcc aaacttgcac aagctgataa
     5101 agcaaaatta aaagtactga atacagaagc tgcgaccctg accagccagt ttaaccagcg
     5161 gttacttgca gcaaataaat ccggcggtct ggttgtgaac gatatcgcgc agctggcagg
     5221 aatgagtgag caagagattg cgctggcggc agaggcggct cgcgagaaag gtctggataa
     5281 caaatggctg attccgctgc tgaataccac ccaacaaccg gcgcttgccg aaatgcgcga
     5341 tcgtgcgacg cgtgaaaaac tgtttattgc gggctggacg cgagcggaaa aaaatgatgc
     5401 caatgatacc cgcgctatca ttcaacgtct ggtagagatt cgcgcacagc aggcgaaact
     5461 gcttggtttt cctcattatg ccgcatggaa aatcgccgat cagatggcaa aaacgccaga
     5521 agcagcactc aactttatgc gggaaattgt tccagcggcg cgtcaacgtg cgagcgatga
     5581 attagcctcc atacaggcgg ttatcgataa gcagcagggc gggtttagcg cgcagccgtg
     5641 ggactgggca ttttatgcgg aacaggtacg gcgggagaaa tttgatcttg atgaggcgca
     5701 gctcaagcca tattttgaat taaacacggt gttaaatgaa ggtgtattct ggaccgcgaa
     5761 tcagctcttc ggtattaagt ttgtcgaacg ttttgatatt ccggtctacc atcctgacgt
     5821 tcgtgtgtgg gaaatttttg atcataatgg cgtgggactg gcgttatttt acggtgattt
     5881 cttcgcccgt gattcaaaaa gcggcggtgc atggatgggc aattttgttg agcaatcaac
     5941 gcttaatgaa acgcatccgg taatttataa cgtctgcaat tatcagaaac ccgctgccgg
     6001 tgagcctgcg ttgttactct gggatgatgt aataacctta ttccatgaat ttggtcatac
     6061 gctgcacggc ctttttgccc gccagcgtta tgccacgctt tccggcacca acacgccgcg
     6121 tgattttgtc gaatttccgt cgcaaatcaa cgaacactgg gcaacgcatc cgcaggtatt
     6181 cgctcgctac gcccggcatt atcagagcgg ggcagcaatg cctgacgaac tgcaacagaa
     6241 aatgcgtaat gccagcctgt tcaacaaagg gtatgagatg agcgaactgc ttagcgccgc
     6301 acttctcgat atgcgctggc attgcctgga agaaaacgaa gcaatgcagg atgtcgatga
     6361 ttttgaattg cgggcgctgg tggcggaaaa tatggatctt cctgctatac cgccacgcta
     6421 tcgcagcagt tatttcgccc atatttttgg tggcggatat gctgcaggtt attacgctta
     6481 tctgtggacg caaatgttgg ccgatgatgg ttaccagtgg tttgttgagc agggcggatt
     6541 aacgcgtgaa aatgggcagc gttttcgcga ggcgatcctt tccagaggta acagcgaaga
     6601 tctggaacgc ctgcatcgac aatggcgtgg taaggctcct cagattatgc cgatgctgca
     6661 acatcgtggc ttgaacatat aaacaccacc aatggctggc ctggagatat cgccaggcca
     6721 gcctggttat agcagtaatt gcagcagctg ggcgagggca aacctcaccg ctaaagctaa
     6781 taccgtttcg cagtcgccag caaaatgcat aaccgcagtg tagttctggc ctttaatatg
     6841 ccacgcgaac cagacggtac ccgctggcgt accatcttca ccgccctccg gtccgccgta
     6901 gccggtaatg gcaatactga catcagcatc cgcacgctct atggcacctg ttgccatttc
     6961 tgctgccact ttctcactca ccgcagaata tcgttcaaga gactgctggc ttacgctgag
     7021 gattttcatc tttgcctgat cggtgaaagt aacaaagcct gcaccgtaaa atttgggtgt
     7081 atcttcagct gcacacaggg cgctagccaa cttaccgccg gtgcatgatt cggcggtagt
     7141 taagcgtaat tgatccgcga taagtctttg actcaacgcg gatgtcaggt tttcaatagt
     7201 gtcggtatct gcgagttgta caattttatc tttattaatg ttcactgttt tttgtccacg
     7261 ctattatatg taaaggtgat ttgctttttc tgtatgctcg tccaatataa taattttcaa
     7321 acatataaat ccgattattc tgaaaacatg tttaagactc ttcaatcttc ttaactttag
     7381 tgagtcaaat tctctgaata tttgaca
//
LOCUS       DAEBXN010000167         7375 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.167, whole genome shotgun sequence.
ACCESSION   DAEBXN010000167 DAEBXN010000000
VERSION     DAEBXN010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7375
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.167"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            51..1541
                     /locus_tag="KJL99_003911"
     CDS             51..1541
                     /locus_tag="KJL99_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HBD4438469.1"
                     /translation="MDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAIEAAQKS
                     TPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGIPLTETF
                     LPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQNRGFDY
                     FMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPFMLYLAY
                     NAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQYDNTII
                     LFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKLISAMDF
                     YPTALDAADISIPKDLKLDGVPLLPWLQDKKQGEPHKNLTWITSYSHWFDEENIPFWD
                     NYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTDLQQKDN
                     LAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1593..2750
                     /locus_tag="KJL99_003912"
     CDS             1593..2750
                     /locus_tag="KJL99_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HBD4438470.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNTLQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2716..2838)
                     /locus_tag="KJL99_003913"
     CDS             complement(2716..2838)
                     /locus_tag="KJL99_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438471.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            3041..4726
                     /locus_tag="KJL99_003914"
                     /pseudo
     CDS             3041..4726
                     /locus_tag="KJL99_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            4764..7124
                     /locus_tag="KJL99_003915"
                     /pseudo
     CDS             4764..7124
                     /locus_tag="KJL99_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
     gene            7181..>7375
                     /locus_tag="KJL99_003916"
     CDS             7181..>7375
                     /locus_tag="KJL99_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438472.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNDLRYMI
                     YPHAHPKDQVNLWLQIHTGSL"
BASE COUNT         2164 a         1615 c         1567 g         2029 t
ORIGIN      
        1 cgccgactga atacagtacc aaaggaaagc caaatattat cgtactaacc atggatgatc
       61 ttggttatgg acaacttcct tttgataagg gatcttttga cccaaaaaca atggaaaatc
      121 gtgaagttgt cgatacctac aaaataggga tagataaagc cattgaagct gcacaaaaat
      181 caacgccgac gctcctttca ttaatggatg aaggcgtacg ttttactaac ggctatgtgg
      241 cacacggtgt ttccggcccc tcccgtgccg caataatgac cggtcgagct cccgcccgct
      301 ttggtgtcta ttccaatacc gatgctcagg atggtattcc gctaacagaa actttcttgc
      361 ctgaattatt ccagaatcat ggttattaca ctgcagcagt aggtaaatgg cacttgtcaa
      421 aaatcagtaa tgtgccggta ccggaagata aacaaacgcg tgactatcat gacaacttca
      481 ccacattttc tgcggaagaa tggcaacctc aaaaccgtgg ctttgattac tttatgggat
      541 tccacgctgc aggaacggca tattacaact ccccttcact gttcaaaaat cgtgaacgtg
      601 tccccgcaaa aggttatatc agcgatcagt taaccgatga ggcaattggc gttgttgatc
      661 gtgccaaaac acttgaccag ccttttatgc tttacctggc ttataatgct ccgcacctac
      721 caaatgataa tcctgcaccg gatcaatatc agaagcaatt taataccggt agtcaaacag
      781 cagataacta ctacgcttcc gtttattctg ttgatcaggg tgtaaaacgc attctcgaac
      841 aactgaagaa aaacggacag tatgacaata caattattct ctttacctcc gataatggtg
      901 cggttatcga tggtcctctg ccgctgaacg gggcgcaaaa aggctataag agtcagacct
      961 atcctggcgg tactcacacc ccaatgttta tgtggtggaa aggaaaactt caacccggta
     1021 attatgacaa gctgatttcc gcaatggatt tctacccaac agctcttgat gcagccgata
     1081 tcagcattcc aaaagacctt aagctggatg gtgttccctt gctgccctgg ttgcaagata
     1141 agaaacaagg cgagccacat aaaaatctga cctggataac ctcttattct cactggtttg
     1201 atgaggaaaa tattccattc tgggataatt accacaaatt tgttcgccat cagtcagacg
     1261 attacccgca taaccccaac actgaggact taagccaatt ctcttatacg gtgagaaata
     1321 acgattattc gcttgtctat acagtagaaa acaatcagtt aggtctctac aaactgacgg
     1381 atctacagca aaaagataac cttgccgccg ccaatccgca ggtcgttaaa gagatgcaag
     1441 gcgtggtaag agagtttatc gacagcagcc agccaccgct tagcgaggta aatcaggaga
     1501 agtttaacaa tatcaagaaa gcactaagcg aagcgaaata actaaacctt catgcggcgg
     1561 atttttccgc cgccttattg agcgagatag cgatgcacgt tacagccaag ccctccagtt
     1621 ttcaatgtaa tctcaaatgt gattactgtt tttaccttga aaaagagtcg cagtttactc
     1681 atgaaaaatg gatggatgac agcactctga aagagttcat caaacaatat atcgaagcgt
     1741 ctggcaatca ggtctatttt acctggcaag gcggtgaacc cactctggct ggcctggatt
     1801 ttttccgtaa agttattcac tatcaacaac gctatgcagg ccaaaaacgt atttttaata
     1861 cattacaaac gaatggcatt ttattgaata atgaatggtg tgcctttctc aaagaacatg
     1921 aatttctggt tggtatctcg atcgatggcc cccaggagtt acatgaccgt tacagacgca
     1981 gtaattcagg taacggtact tttgcaaaag tgatagcagc catcgagcgt ctgaaatcat
     2041 atcaagtaga gtttaatacg ttaaccgtca ttaataacgt taatgtccat tatcctcttg
     2101 aggtttatca ttttttaaaa tctatcggca gtaaacatat gcaatttatc gaattgctag
     2161 aaaccgggac gccgaatatt gatttcagtg gtcatagtga gaacacattc cgtatcattg
     2221 atttttctgt gcctcccacg gcttatggca agtttatgtc aaccattttt atgcaatggg
     2281 ttaaaaacga tgtgggtgaa attttcattc gtcagtttga aagctttgtc agccgttttt
     2341 tggggaatgg gcatacaagt tgtattttcc aggagtcctg caaggataat ctggttgttg
     2401 aaagtaatgg agacatttac gaatgcgacc attttgtcta tccacagtac aaaattggaa
     2461 acattaataa atctgaactc aaaacgatga acagtgtaca actgacagcg caaaaaaaac
     2521 ggatttcagc gaaatgtcag caatgtgcat ataaacctat ctgcaatggc ggttgtccta
     2581 agcatcgtat tactaaagta aacaatgaga ctgtttctta tttttgcgaa ggttataaaa
     2641 tccttttttc aaccatggta ccttatatga acgccatggt tgagttagct aagaacagag
     2701 taccgcttta ccacattatg gatgttgcaa aacaaatgga gaataattaa taacgctctc
     2761 ttttaactcc attcttagcc tccttcttat gaccttaaca aggcacccga cataaattcc
     2821 tgtcgggtgt ttcgtcatca tcatcttccc ttgcgaaaat cctggcttac gtggtatgcg
     2881 tcagggcatt agcaataccc ttgattatca atagcataac cataaccact gaaaattaaa
     2941 aatttctata tatttcatat tgttaaatga tgcttaaata aatttcatca gtaaatcatt
     3001 gccatagata atcattggat ggcacaatca attttcgcta atgataacca ttcccattac
     3061 gttttgtatg ttgatagcaa agtatctctg tctgctgaag cccttttggc tgcgtaagaa
     3121 taataaaact tcagtgttgt taatcattat tatcctcgca atgatccttg gcgtggttaa
     3181 aattcaggtc tggttaaacg actggaataa tgattttttc aatgcgctaa gccagaaaga
     3241 aaccgataag ctttggcagc tcgttctttg gttccctgcc ctgctgggga tctttgtatt
     3301 aatatccgca aacaaaacat ggttaattaa actactgact attcgctggc gcgaatggtt
     3361 aacagattac taccttaatc gctggtttgc agataaaaac tactacttca cgcagatcta
     3421 cggcgagcat aaaaatacag ataaccctga ccaacgtatc gctgaagaca ttctcttact
     3481 gatcaacaaa acgctcagtc tttcatttgg cttcatccag tcacttagca tgctgatcac
     3541 ctttaccgtt attctttggc aaagcgcggg tacgctctca ttcactgttg gtggaacaga
     3601 atggaatatc caggggtata tggtctatac cgtcgtgctc attgtgatcg gtggaacttt
     3661 atttacccat aaggtgggta aacgtattcg tccgcttaat gtggaaaaac aacgtagcga
     3721 agcgacattt cgaactaatc ttgtgcagca taataagcag gcagaactga ttgcgctaag
     3781 caatgctgag tctctacaac gccaggagtt gcgcgacaat tttcatacca ttaaagagaa
     3841 ctggcatcgt ttaatgaatc gtcagcggtg gcttgattac tggcaaaata tttattcgcg
     3901 ctcgcttagc gttcttccct actttctgtt attgccgcag tttattagcg ggcagatcaa
     3961 tctgggcgga ctgatgaaat cgcgccaggc atttatgcta gtatcgaaca atttaagctg
     4021 gtttatttat aaatatgacg aacttgctga actggctgcg gttatcgatc gcttgtatga
     4081 gttccatcaa ctcactgaat agcgccctac gaataagcct aaaaattgcc aacatgcggt
     4141 acaagtggct aatgcgagta ttcgtacgcc tgataataag atcatattag agaacttgaa
     4201 ctttcatgtt tcgccaggca aatggctatt actgaaaggc tactctggtg cgggaaaaac
     4261 cacactgctt aaaacattat cccactgctg gccgtggttt aaaggtgata tttcttctcc
     4321 tgctgacagt tggtatgtgt cacaaacacc gttaatcaaa agcggcttac tgaaagagat
     4381 tatttgtaaa gcacttcccc tgcccgtaga cgataaatcg ttgagcgaag tactgcatca
     4441 ggttggtctg ggaaaattgg ctgcgcgtat tcatgaccac gatcgctggg gagatattct
     4501 ttccagcggc gaaaaacaac gtatcgccct ggcacgatta attttacgac gtccgaaatg
     4561 gatatttctt gacgaaacta cctctcatct tgaggaacaa gaggctatac gcttactgcg
     4621 tttagtgcgt gaaaaactac ccacaagcgg cgtcattatg gttacacatc aacccggtgt
     4681 ctggaacctg gctgatgata tttgtgacat tagcacggtt ttataacaac aataaaagcc
     4741 ctgctgtacg gagatataaa taaatgaagc gagttcttat tcctggcgtc attttatgtg
     4801 gcgctgatgt ggcgcaggcc gtcgatgaca aaaacatgta catgtatttt tttgaagaga
     4861 tgacggtcta tgctcctgtc cctgtacccg taaacggcaa cacgcattac accagtgaaa
     4921 gcatcgagcg tttaccgacc gggaatggca atatcagcga tctgctgaga accaaccctg
     4981 cggtacgcat ggattcaacg caaagtacct cgttgaacca gggagatatt cgccctgaga
     5041 aaatctctat tcacggtgcg tcgccctacc agaatgccta tttgattgac ggtattagtg
     5101 caactaataa cctgaaccca gcgaatgagt ccgatgccag tagtgcaacc aatattagcg
     5161 ggatgtcaca ggggtattat cttgatgtca gcttactgga caatgtgacg ctttatgaca
     5221 gttttgtgcc ggttgaattt ggtcgcttca atggcggggt aattgatgca aagatcaaac
     5281 gcttcaacgc tgatgatagc aaggtgaaat tgggttatcg cactacgcgt ttggactggt
     5341 taacatcgca tatcgatgag aataacaaga gcgcatttaa tcaaggttct tcaggaagta
     5401 cctatttctc tccagatttt aaaaagaact tttatacctt gtcgtttaat caggagctgg
     5461 ctgataactt tggcgttacc gccggtttat cgcgccgcca gtctgatatc acccgcgcgg
     5521 attatgtttc gaatgacggc attgtcgccg gtcgggcaca gtataaaaac gttatcgata
     5581 ctgcattgag caaatttacc tggtttgcca gcgaccgctt tacccacgat ttaaccttaa
     5641 aatataccgg ctccagccgt gattataata ccagcacctt cccgcagtct gatcgcgaaa
     5701 tgggtaataa atcctatggt ctggcatggg atatggatac gcaactcgca tgggccaaac
     5761 tacgtaccac cgttggttgg gatcatatta gtgattatac ccgtcacgat catgacatct
     5821 ggtacaccga actttcatgt acatatggtg atattacagg gcgttgtacc cgtggcggat
     5881 taggacacat ttcccaggct gtagataatt acaccttcaa aacacgcctg gactggcaaa
     5941 aattcgccgt gggtgatgtt tcgcatcaac cctacttcgg cgcggaatac atctattccg
     6001 atgcatggac tgaacgccat aaccagtctg aatcctatgt gattaatgct gcaggaaaga
     6061 aaactaacca taccatttac cataaaggta aaggcagcct gggaattgac aactacacgc
     6121 tgtatatggc ggatcacatt agctggcgga atgtgtcgtt aatgcccggt gtgcgttatg
     6181 actatgacaa ctatctgtca aaccacaata tctccccgcg ctttatgacg gaatgggata
     6241 tttttgctga tcaaacctca atgattaccg ccggttataa ccgttactat ggcgggaata
     6301 ttcttgatat gggattacgt gatatccgca atagctggac ggaatcggta tcaggtaata
     6361 aaaccctgac gcgttatcag aatttgaaaa cgccttataa cgatgaactg gcaatgggat
     6421 tgcagcaaaa aatcgataag aacgttattg cacgcgcaag tgaagcgcat gatcaaatca
     6481 gcaaaagcag tcgtaccgac agcgcgacta aaaccaccat tactgaatat aacaacgatg
     6541 gcaaaaccaa aacgcattcg tttaacctca gttttgaact ggccgaaccc ctgcatatcc
     6601 gccaggtaga tattaacccg caaattgtct ttagctatat caagagcaag ggcaacttgt
     6661 cgttaaacaa tggttatgag gagagcaata ccggtgataa ccaggtggtt tataacggta
     6721 atctggtctc ttacgatagc gttccagtgg cagattttaa taacccatta aagatctcct
     6781 taaacatgga tttcacgcat caaccgagcg ggttagtgtg ggcgaatacg ctggcctggc
     6841 aagaagcgcg taaagctcgc attatccttg gtaagacaaa tgcgcaatac atcagcgaat
     6901 attcagatta caagcagtat gttgacgaaa aactggatag cagcctgacc tgggacaccc
     6961 gcttgtccta gacgccacaa tttctgaaac aacaaaacct gacgatcagt gccgatattc
     7021 tcaatgtact ggatagcaaa accgctgttg atacaacgaa caccggtgtg gcgacctacg
     7081 ccagtggccg tactttctgg cttgatgtca gcatgaaatt ttaatagttc ggattttact
     7141 taaccgggta aattcgcccg gttttcgcat ggagataatt atgagaaacc tctgtttctt
     7201 actgacgtta gtggcaactc tgttgctccc cgggcggctg attgccgccg ccttaccgca
     7261 ggatgaaaag ttaattaccg ggcaactgga caatgacttg cgatatatga tttatccgca
     7321 tgctcatcca aaggatcagg taaatttatg gctgcaaatt cataccggtt cattg
//
LOCUS       DAEBXN010000168         7268 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.168, whole genome shotgun sequence.
ACCESSION   DAEBXN010000168 DAEBXN010000000
VERSION     DAEBXN010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7268
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.168"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            18..1076
                     /locus_tag="KJL99_003917"
     CDS             18..1076
                     /locus_tag="KJL99_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005119414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBD4438473.1"
                     /translation="MSPEQRRAICEAFESANNTHGLNLTAHKYPGLRGTLQTASTDCD
                     TIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDLNDRYVYFYEPVNVEYF
                     AMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQNVMSLLTPVYVAGQLKG
                     IVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIIINQSEDNLFQYVSYVHD
                     LPGGIRVSLSIDILYFITSSRKSVLFWILTALILLNMVRMHFRLYQNVSRENISDAMT
                     GLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDTLGHQEGDLAITLHDAY
                     KASDERLYVNKQNKNSRS"
     gene            1119..1622
                     /gene="yeaK"
                     /locus_tag="KJL99_003918"
     CDS             1119..1622
                     /gene="yeaK"
                     /locus_tag="KJL99_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HBD4438474.1"
                     /translation="MTEMAKGSVTHQQLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(1623..1727)
                     /locus_tag="KJL99_003919"
     CDS             complement(1623..1727)
                     /locus_tag="KJL99_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438475.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERIG"
     gene            1897..2343
                     /locus_tag="KJL99_003920"
     CDS             1897..2343
                     /locus_tag="KJL99_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HBD4438476.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(2300..3118)
                     /gene="nimR"
                     /locus_tag="KJL99_003921"
                     /pseudo
     CDS             complement(2300..3118)
                     /gene="nimR"
                     /locus_tag="KJL99_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
     gene            3215..4395
                     /gene="nimT"
                     /locus_tag="KJL99_003922"
                     /pseudo
     CDS             3215..4395
                     /gene="nimT"
                     /locus_tag="KJL99_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416305.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
     gene            4450..4797
                     /locus_tag="KJL99_003923"
     CDS             4450..4797
                     /locus_tag="KJL99_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HBD4438477.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYRAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(4819..5073)
                     /gene="yoaF"
                     /locus_tag="KJL99_003924"
     CDS             complement(4819..5073)
                     /gene="yoaF"
                     /locus_tag="KJL99_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HBD4438478.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            5256..6281
                     /gene="dgcP"
                     /locus_tag="KJL99_003925"
     CDS             5256..6281
                     /gene="dgcP"
                     /locus_tag="KJL99_003925"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HBD4438479.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVLWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLHEANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            6314..6412
                     /locus_tag="KJL99_003926"
     CDS             6314..6412
                     /locus_tag="KJL99_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HBD4438480.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGCYAAPGA"
     gene            6415..6489
                     /gene="yoaJ"
                     /locus_tag="KJL99_003927"
     CDS             6415..6489
                     /gene="yoaJ"
                     /locus_tag="KJL99_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HBD4438481.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(6650..6841)
                     /locus_tag="KJL99_003928"
                     /pseudo
     CDS             complement(6650..6841)
                     /locus_tag="KJL99_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004995861.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7021..7137)
                     /locus_tag="KJL99_003929"
     CDS             complement(7021..7137)
                     /locus_tag="KJL99_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBD4438482.1"
                     /translation="MRNVFRCHIPRYEAIIARYLDMEPAQIWPSRYMEKTSC"
BASE COUNT         1753 a         1763 c         1770 g         1982 t
ORIGIN      
        1 ctgctgagca gtcggaagtg tcgccagaac agcggcgcgc catctgcgag gcttttgagt
       61 ctgccaataa tacccatggc ttaaacctga ctgcccataa atacccgggc ttacgcggca
      121 cactacaaac cgcatccact gactgcgaca caattgtgga agctgcagca ctattacccg
      181 cttttgatca ggcagtggaa ggcaaccgcc accaggatga ttacggttca ggtcttggga
      241 tggccgaaga gaaatttcac tattatctcg atctcaatga ccgctatgtc tatttttatg
      301 agccggttaa tgttgaatac tttgcgatga ataactggtc cttcctgcag tcaggaagta
      361 ttggcatcga tcgcaaagat attgaaaagg tatttaccgg gcgtaccgta ttgtcgagca
      421 tttaccagga tcagcgtact aaacagaacg tgatgagttt gctgacgccg gtatatgtcg
      481 cagggcagct aaaagggatt gtgctgctgg atattaacaa aaacaatttg cggaatatct
      541 tttacactca tgaccgccct ctcctctggc gttttctcaa tgtcacgcta accgataccg
      601 attcggggcg cgacattatc atcaaccaga gcgaagataa tctgttccag tatgtcagtt
      661 acgtccatga cttacctggc ggcattcgtg tctcgttatc cattgatatt ctttacttta
      721 tcacgtcttc gaggaaaagc gttctgttct ggattttgac ggcgttaatt ttgctgaata
      781 tggtccggat gcacttccgt ttataccaaa atgtgtcgcg agaaaatatt agtgatgcga
      841 tgactggact gtataatcgc aaaattttaa cccctgaact ggagcagcgg ttgcagaaac
      901 tggtgcaatc cggttcttcg gtgatgttta ttgcaattga catggacaag ttaaagcaaa
      961 taaatgacac cctcggtcat caggaggggg atttagcgat tacgttacat gatgcgtata
     1021 aagcttccga tgagcgttta tatgtcaata agcagaacaa aaacagccgt tcatgataac
     1081 cttctgtggt tgtttgcttg taatctcagg agcgtgaaat gactgaaatg gctaaaggaa
     1141 gcgtgacgca tcagcagtta atcgctttat tatcacaaga aggtgctaac ttccgcgtcg
     1201 ttacccatga agcggtaggt aaatgtgaag cggtgtcaga aattcgtggt accgctctag
     1261 gccagggtgc taaggcactg gtatgtaaag tcaaaggcaa tggcgtaaat caacatgttc
     1321 tggcgatcct cgcagccgat caacaggccg atctgagcca actcgccagc catataggcg
     1381 gattacgcgc ttcgttggcc agcccggcgg aagttgatga actcacaggc tgtgtcttcg
     1441 gcgcgatccc ccctttcagc ttccatccaa aactcaaact ggttgccgac cctctactct
     1501 tcgaacgatt tgatgaaatc gccttcaatg cagggatgct ggataaatcc gttattttga
     1561 aaaccgcgga ttatttgcgc attgcgcaac cagaactggt caatttccgc cgcactgcgt
     1621 agctagccta tccgttcaat aagcagaacg gacaaaacca gcacaaccgc aatagcaaaa
     1681 aacgatgatg tgataatcag tgtctcgacg aacatttgat cgttcatagc atgcccccgg
     1741 gagatagctg ttttcacgtt attgttagcg tgcacaaatg gcagtttgat gacagttcgc
     1801 ctgatttttt atgcaaaaaa cgtaaagatt ttctacttcc ttcctgcagc aagcataaag
     1861 taagcaggct tattattttt tggcaaggaa accacgatgt ttgatgtcac tttgctgatc
     1921 ctgctcgggt tagctgcgct gggctttatc agtcataaca ccactgtcgc cgtttcaatt
     1981 ctggtgttga tcattgtccg cgtcacaccg ttaagcacct ttttcccgtg gattgaaaaa
     2041 caggggctaa gtatcgggat aatcattctg acgattggcg ttatggcccc catcgccagc
     2101 gggacgctac cgccctcaac cttgattcac tcgtttctga actggaaatc gctggtggca
     2161 attgcagtag gcgttattgt ctcctggctg ggtgggcgcg gcgtgacgtt aatgggcagc
     2221 cagccgcaac tggttgctgg gctgctggtg ggcactgttt taggcgtagc gctgtttcgc
     2281 ggcgtaccgg ttggcccgct tattgccgcg ggtctggttt cgctgattgt ggggaaacag
     2341 tagttaatcc cgcaatatat cggcctggcg tttgccccaa gcctttctta aacatagtaa
     2401 taaaggccgt tgttgaatca taccccagcg tatgagcaac tttctgtaca gtatcgcctt
     2461 tcactaatcc ctgaagcgcc ataatcagct gtaactgctg acgccactgg cgaaaactca
     2521 accccgtctc tttcactatc aggcgggcaa gattgcgttc actcatcgca aaaaagccag
     2581 cccattgccc caataccccc cactcgacgg gccctttcgc catcatctcc accatcgtgc
     2641 ggattttagg atgagaagaa acgggcaggt gcagctgttg ttgtggctgc tgagggagtt
     2701 catcaaacag gacctgaatt aatctgcggg tcatcggctc agcacgctga gtcgtcgtcc
     2761 tgtttgccag ggttaaaatc aattcccggc ataagggcga tattttcaat gtgcaacatg
     2821 gtcggcattg tcactgcgct gggttcgata aacagaaaac agagttcagc gttagctgtc
     2881 acttgattgc tatgcttcac cccgcccggt atccaaaccg catattgtgg cggcaccatc
     2941 cacaaagcat tttccaccgt acaggtaatt gcaccatgta atgccagtat cagctgtcct
     3001 ttgcggtgct gatgtacagg actggttaac tcatccgttc cggcgtgaat acaaaatgcg
     3061 accgcggctt catggtgtcg gtgggttcat agccattgag attcaacctg tgcatcattt
     3121 tgtccgaact tagcgataat ttgtcatttt agcttgattc aacataacaa taaaaacggt
     3181 aaggtacagc ctcgtttgta acaatgagaa gcatatgacc tgttcaactt cattaagcgg
     3241 caaaaacagg attgtcctta tcgctggcat tctgatgatt gccacaacat tacgcgtcac
     3301 ctttaccggc gcagcgccgt tactggatac gattcgttcc gcttactcgc tgacgacagc
     3361 gcaaaccggc ttattaacca ccctgccatt attggccttt gcgctaatct cacctctggc
     3421 tgccccggtg gcgcgacgtt ttggtatgga acgtagcctg tttgccgcgt tacttttgat
     3481 ctgtgcaggt atcgcaattc gttctctccc ttcgccttac ttattatttg gcggtacagc
     3541 ggtcattggc ggtgggattg cattaggcaa tgtcttactg ccaggattaa ttaaacgcga
     3601 tttccctcat tccgtcgcca gacttaccgg cgcatattcc ctgacaatgg gagctgcagc
     3661 ggcactggga tcggctatgg tcgtgccgct ggctttgaac ggttttggct ggcaaggcgc
     3721 gttgttcatg ctgatgtgtt ttcctctgct ggctcttttt ttatggctgc cacagtggcg
     3781 aagtcagcaa catgcaaatt tgagtacctc acgcgcctta catactcggg gtatctggcg
     3841 ttcgccactt gcctggcagg taacattgtt tcttgggatc aactcactgg tctattacgt
     3901 gattattggc tggcttccgg cgatcctcat cagtcacggc tatagcgaag cacaggcggg
     3961 ttcactgcat ggtttgctgc aactagccac agcagctccc ggtttgctga tcgcactttt
     4021 cttacatcat gtgaaagacc agcgtggtat tgcagcgttc gttgccttga tgtgcgcagt
     4081 gggcgcggtt gggctctgct ttatgccagc gcacgcgatc acctggactc tgcttttcgg
     4141 ttttggttcc ggcgcaacaa tgatactggg gttgacgttc attggtcttc ggcaagttct
     4201 gcgcatcagg cggcggcact ctcggggatg gcacaatccg tcgggtattt gctggcagcc
     4261 tgtgggccgc cgctaatggg taaaatacac gatgctaacg gtaactggtc cgtaccactg
     4321 atgggtgttg caatactttc actactgatg gcgattttcg gactttgcgc cgggagagac
     4381 aaagaaattc gctaatatcc ggtgctatag tgacgtgaca aatcttgcgt gaaagggaga
     4441 acaaacacga tgaatattca gtgcaaacgc gtttatgatc cggctgaaca gagcgatggt
     4501 tatcgcgtac tggtcgaccg cctctggccg cgcggtatca aaaaaaccga tttagccctt
     4561 gatgagtggg ataaagaaat cacgccgtca acggaactgc gcaaagcctt tcacggcgaa
     4621 gtcgtcgatt ttgcaacctt tcgcgagcaa tatcgcgcag aactggcgca acacgagcaa
     4681 gaaggaaagc ggctggcgga catcgccaaa aaacagccgc tgaccctgct ctacgcagca
     4741 aaaaacacca cgcagaacca tgcgctggtg ctggccgact ggctacgtag cttgtgattt
     4801 tagtacggca tccggcggtt atttttcacc agccggatgg tcccgtcgcc acaatgccca
     4861 ttcatcaatc gtttcaccgc ccggtaattt gcaattgttg ctgacccctt gcgctgtctg
     4921 cactggaatg agcgtcccgc ccttctgctg gcaatagacc gacgccggat ttgccatacc
     4981 aatctgcggc ggtttaggtg cttctggctg agaaggggtt gaacaaccag ccaggaccag
     5041 caagcaaggc agaacaaaac tgataatttt catttattga tctcacatat ttatccaagc
     5101 ttagagtatc gcggtatcgt tttgttttgc agcactattt ttattacatt cactcaaaac
     5161 atattacgtc ttgtttcatc tttgttgatg atgttttatc atgcctgcaa agattaaata
     5221 atcagcactt acccgccgta tcctggagtt gttccgtgtc agatcagatt atcgcccgcg
     5281 tctcgcaatc ccttgccaaa gaacagtcac tggaaagtct ggtccgacag cttctggaga
     5341 tgctggaaat ggtcactgat atggaatcaa cctacctgac caaagtggat gtcgaagcgc
     5401 gcctgcagca tattatgttt gcccgtaaca gccagaaaat gcacatcccg gagaatttta
     5461 ccgtcttgtg ggattactcg ttatgcaaac gcgccattga tgaaaactgc tttttcagcg
     5521 atgaagtccc tgaccgttgg ggcgactgta ttgcggcacg caatcttggc atcaccacat
     5581 ttctgagcac gccaattcac ttaccggatg gatcattcta tggcacgctt tgcgccgcca
     5641 gcagtgagaa gcgccagtgg agtgaacgcg cggaacaggt tttacagtta ttcgccggac
     5701 tgattgcaca atatattcaa aaagaggcac tggttgaaca gctgcacgaa gccaatgctg
     5761 cgctgattgc gcaatcgtat accgactcgt taaccgggct accgaatcgg cgggcgattt
     5821 ttgaaaatct gacgacgctg ttttccctcg cccggcatct taaccataag ataatgatcg
     5881 cgtttatcga tctggataac ttcaaattaa tcaatgatcg ttttggtcat aatagtggcg
     5941 atctgtttct cattcaggtt ggcgagcgcc ttaatacgct ccagcaaaat ggcgaagtta
     6001 ttggtcgtct cggtggtgat gagtttttgg ttgtttcact aaacaacgag aatgcggata
     6061 tttcgtcgct gcgagaacgt attcaacagc aaatacgtgg agaatatcac ttaggtgatg
     6121 ttgatttgta ttatcccggt gccagtcttg gcatagtaga agtcgatcct gaaacaaccg
     6181 atgcagacag tgccctgcat gctgccgata ttgcgatgta tcaggagaaa aaacacaaac
     6241 agaaaacacc ttttgtcgcg catccagcgc tacattcctg aggcgtattc acatcctttt
     6301 gattggtgat aacatgcgaa tcggtattat ttttccggtt gtaatcttca ttacagcggt
     6361 cgtattttta gcatggtttt ttattggctg ctatgctgcc ccgggagcat aaagatgaaa
     6421 aaaacaacga ttattatgat gggtgtggcg attattgtcg tactcggcac tgagctggga
     6481 tggtggtaac gtcacctcta aaaaatagca aaggctgcct gtgtgcagcc tttgtgcaat
     6541 ttaagcgtta acttttaatc ttcctgtaga taaatagcac gacaatcgca ccaataacgg
     6601 ctgaagcggc gcacgaaaaa cgctttcgcg tttttcgtgc gccgcttcac ccctggttca
     6661 gcatttcgtc tgcagtctga accgcaggca accatttttc agcaagtctg cgctgggaat
     6721 cgctggcttt atcccatttg ctccacagtg cctcacgatc gtaatcgtgc gcttccagcg
     6781 tggggcgggc gatttcaaaa tacccctagc ttgtttctat ttgctcagat tttaataaca
     6841 actcagcacg tgctgccgct ggtagaactg atatatgaaa ttccaaagcc tttgaacccg
     6901 tacgcttgcg acgtagttct ggatgattgg ctgcaagctt ttcaagattg cggcgaatac
     6961 tcacctcatg tttaggaaag catgacattc ctacgcattc ttgcgctgtt acccaatggg
     7021 ttaacatgat gttttctcca tataccgaga aggccaaatc tgggctggtt ccatatccaa
     7081 atatctagca atgattgctt cgtatcgtgg tatatggcag cgaaatacgt tacgcatagt
     7141 gtcaggcttt aagcctgcct tgaggtgtac tggcaatagc ggatactacc atttgttctt
     7201 tttttaagca gttatctgat gatatttttc cctgaaggct gccggggaga tattccccag
     7261 acgagagt
//
LOCUS       DAEBXN010000169         7258 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.169, whole genome shotgun sequence.
ACCESSION   DAEBXN010000169 DAEBXN010000000
VERSION     DAEBXN010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7258
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.169"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            28..879
                     /gene="hchA"
                     /locus_tag="KJL99_003930"
     CDS             28..879
                     /gene="hchA"
                     /locus_tag="KJL99_003930"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HBD4438483.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHSDNPLNGYSICAFPDAADKQTP
                     DIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKRLTGDSPFAANALGKLAAQE
                     MLAAYAS"
     gene            complement(987..2345)
                     /gene="hprS"
                     /locus_tag="KJL99_003931"
     CDS             complement(987..2345)
                     /gene="hprS"
                     /locus_tag="KJL99_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HBD4438484.1"
                     /translation="MKRLSITVRLTLLFIFLQSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHSDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQIRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVKCNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDANGSLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLSKHNVFRITLPQ
                     RN"
     gene            complement(2345..3016)
                     /gene="hprR"
                     /locus_tag="KJL99_003932"
     CDS             complement(2345..3016)
                     /gene="hprR"
                     /locus_tag="KJL99_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HBD4438485.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            3149..3562
                     /gene="hiuH"
                     /locus_tag="KJL99_003933"
     CDS             3149..3562
                     /gene="hiuH"
                     /locus_tag="KJL99_003933"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HBD4438486.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            3671..4675
                     /gene="msrP"
                     /locus_tag="KJL99_003934"
                     /pseudo
     CDS             3671..4675
                     /gene="msrP"
                     /locus_tag="KJL99_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
     gene            4676..5311
                     /gene="msrQ"
                     /locus_tag="KJL99_003935"
     CDS             4676..5311
                     /gene="msrQ"
                     /locus_tag="KJL99_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HBD4438487.1"
                     /translation="MRLTAKQVIWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQSMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQAHNKLSL"
     gene            5569..6219
                     /gene="zinT"
                     /locus_tag="KJL99_003936"
     CDS             5569..6219
                     /gene="zinT"
                     /locus_tag="KJL99_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HBD4438488.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYP
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
BASE COUNT         2057 a         1647 c         1519 g         2035 t
ORIGIN      
        1 taactaagca acaataagga atacactatg actgttcaaa caagtaaaaa cccgcaggtc
       61 gatattgctg aagataatgc gttcttccct tcagaatatt cgcttagcca atatacgagt
      121 cccgtctctg atcttgatgg cgtggactat ccaaaaccgt atcgcggtaa acataaaatt
      181 ctggtgatcg ccgcggacga acgttatttg ccgaccgata acggaaaact gttctcgacc
      241 ggtaaccatc cgattgaaac gttgctgccg ttgtatcatc tccatgctgc aggtttcgaa
      301 ttcgaagtgg cgaccatttc cggtctgatg accaagtttg aatactgggc tatgccgcac
      361 aaagatgaaa aagtaatgcc attctttgaa cagcataaat cgttattccg taatccgaag
      421 aaactcgcgg atgttgtcgc cagcctcaac gctgatagcg aatatgcagc aatctttgtt
      481 cctggtgggc atggcgcact tattggttta ccagaaagcc aggacgtggc tgccgcttta
      541 cagtgggcaa tcaaaaatga ccgttttgtt atctcccttt gccacggtcc ggcggctttt
      601 ctggcacttc gccacagcga taacccactg aatggttatt ctatttgcgc attcccagac
      661 gccgcagaca aacaaacgcc agatattggc tatatgccgg gtcatctcac ctggtacttc
      721 ggcgaagagt tgaagaaaat gggcatgaac atcattaatg acgacatcac cgggcgagta
      781 cataaggacc gtaaacgtct caccggcgac agtccttttg cagcgaatgc gttgggtaaa
      841 ctggcggcgc aggaaatgct ggcagcttac gcgagttaat ccctgttaca ttgtgatgta
      901 agcgcataat caatcgcagc cacgttaata ctcacgacgt ggctgcgttt tttcacggtt
      961 aacttatggc gtactgaagc ccgatgttaa tttctttgcg gtaacgtaat tcggaacacg
     1021 ttatgcttac tgagatagtg atacgtagca cttcctccat gtaattcggc aatcgctttg
     1081 actaaagaaa ggccaagtcc ctgacctacg gaatgacgcg aattatctcc ccgccaaaat
     1141 ctacggaaga gtttttcagg ctcattaatt ttcgttccag gactggcgat atcaatatta
     1201 agagagccgt tggcatcaag aaaactggtt atatgaatac gcgatttttc tggtgaatat
     1261 cgaatggcgt taacaataag attcgataac attcgttgca gtaaaatttt atccgcaaag
     1321 atttgttgat tgcacttgac cttaaagcaa atctctttct cgtctgaaag atattcaaga
     1381 tagtccaaaa gattttcaac ttccttattg agagaaagcg aatccagttt caccaaaaca
     1441 ttgtttttgt ctgccctggc aagaaacagt atattctctg ttaaccgcga aatattttcc
     1501 agctcttcaa tgtttccggc aattgttttt tgatattcag cgatacttct gatttgactg
     1561 agcgtaacct gattctgacc cagtaatgca ttaattggcg ttctaagttc atgagcgaga
     1621 tcgtcagcaa attgactaag gcgctcaaaa tctttgacta aagcatgatg cattttattc
     1681 aacgcctgcc caagaggttt tagttctctc ggtaatgcgt taacctcaac cggcactcgg
     1741 ctatcgttat aattcagcgc ttccgttacg ccactcaact ttttgatctc tcgcaacccc
     1801 gtcctgatta acaacggact taatactgag cacagtataa tggcgacaat gcaaattata
     1861 atgctattaa ttttatactg ttcaagcata ttatgtctgg ctgaagccaa tttagccaca
     1921 gtaaccgtta atgatggcga aacttcatca atgttaattc gtaaagcatc tatctctgta
     1981 tcattaataa tgcttctgta aatgcctgct gcgctgattg tctcactagc aggtatgtta
     2041 tttaacatgt catcactgac atttgtccgg ttaacaattt tattgatgcc atcactatga
     2101 atgatcaaaa tatcctgact aacatccatc atccggttaa agtacacagg taacgtatct
     2161 ggatttaccc catcaattaa caactgcttg atctgcgctg tccggttaat gagtgttgta
     2221 tcatcgcgcc atttcaactc acttgccagg ccattataga gagtccatac aattccagcg
     2281 ccagcaacag actgcagaaa tataaaaagc aaggttaaac ggacggttat agataatctt
     2341 ttcattattt ttttaccgct acgaatgaat agcccatccc ccggattgtg gcgattagct
     2401 tttcaggaaa aggatcatca acttttgcgc ggagcctgcg aatggcgaca tccaccgtat
     2461 tggtatcact atcaaagttg attccccaaa tttcactcgc aataaccgtt ctgggtataa
     2521 tctcgccagc tctggaggcc agtagccaaa gtaactgaaa ctccttgcgc gtcagtgtaa
     2581 tactgatatt gtccctgctg acactttgac tgacagagtc catttttaag ccactgattt
     2641 ctaatgttga attcagagcg tgatgttgcc ttaattgtgc ccgaaccctt gctagcaact
     2701 cagaaaatga aaaaggtttt accagataat catttgcccc actgtccaac cctctgactc
     2761 tgtcatcgac agaatccctt gcagtaaggc aaataacagg ggtttgcttt gccgttctta
     2821 acgtttgtaa gatctgccag ccatccatac ccggaagcat aatatccaga atgatcaatg
     2881 cataatcatc cttcagcgca agataaagcc catctctgcc atcagaaacg gcatcaatga
     2941 cataacccgc ttcggaaagc ccctgcgtta cccattcctg ggtcctttga ttatcttcaa
     3001 taagtagaat cttcatgaaa ataatatgcc ataaatcata tgttatagaa acaacctggt
     3061 tcattacaaa attgtaatgc tgctgtaagg ttaccctgga cgttttttcg ctatcctcaa
     3121 aactcattca catgacaagg atataaacat gttaaagcgt tatttagtac tctcggtagc
     3181 aacggcagcc ttttcattac cttctttggt ttatgccgca caacaaaaca ttcttagcgt
     3241 gcacattttg aaccagcaaa caggaaaacc cgctgccgac gtgacagtca ctcttgaaaa
     3301 gaaagcggat aacggctggt tacaacttaa taccgccaaa acagataagg atggacgaat
     3361 taaggcactg tggcccgagc aaactgcgac tactggcgat taccgtgtcg tatttaaaac
     3421 cggggactat ttcaagaaac aaaatcttga aagtttcttc cctgagattc ccgttgaatt
     3481 tcatattaat aaagtgaacg agcattatca tgtgcctttg ttactcagcc aatatgggta
     3541 ttcaacctat cgtggcagtt aatgcgtaaa tatgtaaaag ctgatctctg ctactgttag
     3601 ggaggtgact ccctgacaga ttaacagtaa acagctcttg ttggttaacg acaaaaaagt
     3661 gtgatggctt atgaaaaaga agcaattttt aaaagaatca gatgttacgg ccgagtcggt
     3721 attctttatg aagcgtcggc aggtgttaaa agcactgggc atcagcgcag ctgcattttc
     3781 tttgcctcac gctgcgcatg ccgatctgct tagctggttt aaagggaacg atcgtccgcc
     3841 cgcccccgcc ggaaaaccgc tggagttcag caagcctgcc gcctagcaaa ataacctgcc
     3901 actgacgcca gcagataaag tctccggtta taacaacttc tatgaattcg ggctggataa
     3961 agacgatccc gcggctaatg ctggtagcct gaaaaccgat ccatggacac tgaaaatcag
     4021 cggcgaagtg gcaaaaccat tgaccctcga tcatgatgat ttaacccgtc gcttcccgct
     4081 ggaagagcgt atttatcgta tgcgctgcgt ggaagcgtgg tcgatggtgg tgccgtggat
     4141 tggttttccg ctacacaaat tgctggcgct tgccgaaccc accagcaatg cgaagtatgt
     4201 cgctttcgaa acgatttatg cgccggaaca gatgcctggc cagcaggacc gctttatcgg
     4261 cggcgggctg aaatatcctt atgtcgaagg attgcgtctc gacgaagcaa tgcatccgct
     4321 cacactgatg accgtaggtg tttatggcaa ggcgttacca ccgcaaaatg gcgcgccggt
     4381 gcgactgatt gtgccgtgga aatatggctt taaagggatt aaatcgatcg tcagtattaa
     4441 gctgacccgc gagcgtccgc caaccacctg gaatctggca gcgcctgacg aatacggttt
     4501 ttacgccaac gttaatccgc atgttgatca cccgcgctgg tcacaggcta ccgaacgatt
     4561 tattggttca ggcggcattc tcgatgttca gcgccagcca acactactgt ttaatggtta
     4621 cgccgaccag gtggcatcgc tgtatcgtgg cctggatttg cgggagaatt tctaaatgcg
     4681 tctgacagca aaacaagtga tatggctgaa agtttgcctg catcttgccg gattgttgcc
     4741 gtttctctgg ctggtctggg cgatcaatca cggtggactg ggtgccgatc cggtgaaaga
     4801 tattcagcat tttaccggtc gcactgcact gaaatttttg cttgcggcct tgttaatcac
     4861 acctctggca cgctacgcaa aacagccgtt attgatacgc actcgccgcc tgttaggctt
     4921 atggtgcttt gcctgggcga cactgcattt aaccagttac gcattgctgg agttgggcgt
     4981 gaataatctg gcgttattag gtaaggagtt aattacccgg ccttatttaa cgttaggaat
     5041 tatcagctgg gttattttgc ttgctttagc gttcacttca acccagtcga tgcagcgaaa
     5101 actcggcaaa cactggcaac agttgcataa cttcgtctat cttgtcgcga tcctggcccc
     5161 aatacattat ctgtggtcag tgaagattat ttcaccgcag ccactcatct acgccgggct
     5221 ggctgtactg cttttagcct tacggtataa gaagttgctt tcccttttca accggttacg
     5281 caagcaggcc cacaacaaat tatctctata acttcatatt gccgacaaac ttcgcgttat
     5341 cccggccgga tgcggcgcga gcgccctatc cggcccacac agatcgcatc aagttatgtt
     5401 tcgtttgtca tcaacctttc atcaccagaa taatttattt tatttcaata agatagttca
     5461 atacacctga gaataccatg ctctcatttc ctaatgagtt gttgcatttt ttcatatgtt
     5521 acaatataac attacacata atgtacatta actttggagg aaattgtttt ggcgattcgt
     5581 cttcataaac tggctgttgc tttaggtgtc tttattgtta gcgctcctgc cttttcgcat
     5641 ggtcatcatt ctcatggcaa acccttaaca gaggtcgaac aaaaagctgc taatggtgtt
     5701 tttgatgata ccaatgtaca aaaccgaacg ctcagtgact gggatggagt ctggcaatcc
     5761 gtttaccctt tactgcaaag tggcaaactt gatcccgtct ttcagaagaa agcggatgca
     5821 gataaaacca aaacatttgc tgaaattaaa gattattatc gcaaaggtta tccgacagat
     5881 atcgaaatga ttggcattga ggacggcatt gtcgaattcc atagaaataa tgaaacaaca
     5941 tcctgtaaat atgattacga tggatacaaa atactcacct ataaatcagg caagaaaggc
     6001 gttcgttact tattcgaatg taaagatcct gaaagcaaag cccctaaata tatacaattt
     6061 agcgatcata taattgcacc acgaaaatca tctcattttc acatttttat gggtaatgat
     6121 tcccagcaat ctttattgaa tgaaatggaa aactggccaa cgtattatcc ctatcagttg
     6181 agtagcgaag aagtggtcga ggagatgatg tctcattgat ttttttgcgc tgacttcagt
     6241 tatttaacag aacagtgcct ggtagtaaca acaataattt accaagacaa ataagtaaga
     6301 caaactcaga cacgctcata cttgagtcgt gtctttttta ttatgtctgt agaaaaaaac
     6361 tgagaaaaag gctaacaata aatacacaac tcagccatac agactggcag cgcaaatgtt
     6421 tccaggagca agtcatctgt catggtaaag ctaaccagaa atataggtta attttggtgt
     6481 ttaaaccttt tttacttgtt agtatgatac aaaggctttc aaaaaagctg ggaaattcaa
     6541 caactcttgg aaattatgga ctttgtcccg cggctttaat ctgacggccg cgttcctttt
     6601 ttggctcaat tattgacccc ttctctacgc aacttcagtt cccgccagca actttgcggc
     6661 agtttcatag gatcaatgtc taaaagaata atggtgaccg ataagaaaac gactgaataa
     6721 ctgtagattt tcgctcgaaa ccttcctgtc agctccatag cgaatcaagc gctgaatgtc
     6781 acagtatcga acagaaaaca gtgacgatct aacccttcaa gaatattcta cgattgttct
     6841 gtttaggaaa agcaaggcgg gaagtcggga gataagtcat tgataaaatg gcggagagag
     6901 ggggatttga acccccggta gagttgcccc tactccggtt ttcgagaccg atgatacaga
     6961 gtcgaaaaat caaagagtta aaaatttttc ttggaaaaaa tgtcttaaaa tcgtacacga
     7021 aaaatcaatg aattacataa cattaaagaa gaatattcca agccttcttg agtccaccac
     7081 ctgttttcag gtgtcattca gacgtgattt gccccagatt gggaaagcac gcttttttct
     7141 cagcttattc cgttttgcct gtgtgttgaa taattccact gcagttatat aaaatcggta
     7201 acggctggaa atcattcaat actcgcacta tcgaaagttt accagccaac cgcagcac
//
LOCUS       DAEBXN010000170         7183 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.170, whole genome shotgun sequence.
ACCESSION   DAEBXN010000170 DAEBXN010000000
VERSION     DAEBXN010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7183
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.170"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(296..1198)
                     /locus_tag="KJL99_003937"
     CDS             complement(296..1198)
                     /locus_tag="KJL99_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HBD4438489.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIDPEYIAAVSACSMREGIVLYNNE
                     GTPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRAWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTLVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     TDNKQCTLSEARHQLPITPFRMPS"
     gene            complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="KJL99_003938"
     CDS             complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="KJL99_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HBD4438490.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            2479..4014
                     /gene="lsrA"
                     /locus_tag="KJL99_003939"
     CDS             2479..4014
                     /gene="lsrA"
                     /locus_tag="KJL99_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HBD4438491.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAVGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGSNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLFISSDMEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4008..5036
                     /gene="lsrC"
                     /locus_tag="KJL99_003940"
     CDS             4008..5036
                     /gene="lsrC"
                     /locus_tag="KJL99_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HBD4438492.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGALVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGIS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFILNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5036..6028
                     /gene="lsrD"
                     /locus_tag="KJL99_003941"
     CDS             5036..6028
                     /gene="lsrD"
                     /locus_tag="KJL99_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HBD4438493.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6040..7063
                     /gene="lsrB"
                     /locus_tag="KJL99_003942"
                     /pseudo
     CDS             6040..7063
                     /gene="lsrB"
                     /locus_tag="KJL99_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
     gene            7090..>7183
                     /locus_tag="KJL99_003943"
     CDS             7090..>7183
                     /locus_tag="KJL99_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="involved in autoinducer 2 transport and processing;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase LsrF"
                     /protein_id="HBD4438494.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGA"
BASE COUNT         1676 a         1812 c         1876 g         1819 t
ORIGIN      
        1 tttcgtatgc agtgtctatg cgacagacac tgcgctctta gcatggggca gggaataacc
       61 aggctatttc tccatgcaca ggccaattat gaaagaggag aggcttaagt tcttgaaccc
      121 tgaacgaata acgactacca gaaaagcact tcgcatcact cagacatgat ttaacataat
      181 atacattatg cgtagtaata ttgtagtagc taaattgaga tgtccgtctc tgactaaata
      241 gacatgtccg cgctacagtc tcaccacagc gatcactgct ttcaaaggat gggactcatg
      301 acggcatacg gaacggagta atcggcaact gatgccgggc ctcgctgagc gtgcactgct
      361 tattatccgt gaacgtgccg ccaagaaccg cggtttgtgc cggacgcaca acgcctaacc
      421 caaggcaacc aagctgcacg tcgcctcctc caacgaccac cagagtgccc gccttcagac
      481 cgcagagttc cgccgcttgt gaacttacca cgcccagcaa tgtgccggtt tctttgacag
      541 gagaaagaat atcggcacgc aggccagcca tatccagcaa tgcaggtttc caggcacggg
      601 tggttagatc aagaagtccc gtggtgccag cgttagaggg atccaccgcc agttcgccgc
      661 tgagcatata ggccagccag tcgctgatca tggtgatggt tgatgcctga cggtaaatat
      721 cggaacgatg gtgcgccagc caaagtaatc tggggatggc acttaaagcc agtgtttgtc
      781 cggtcgctcg ataaacttcg ttttcaaagg tattgttgtg cagttcttta agttcgctaa
      841 cttcgcgtgc cgctctggca tccacattgg cgcaggccca gatcggtgtt ccttcattgt
      901 tatataaaac aatgccttca cgcatagaac atgccgaaac ggcagcgata tactccgggt
      961 ctatgccggc gttgtgcagc gcctggcgca tacactcaca cgccagttgc cagtttttgt
     1021 tgagatcaaa ttccatagaa ccaggaacgt ccggtactgc cagatgccgc cactccgcct
     1081 gtcccactgc tatttgattg ccttccaggt cgaatatcac agcccgaata cttccggtgc
     1141 ctgcatccag cgccatcagg tagtactttg attctgaagg ggtaaagagt cgagccatta
     1201 gattatcctc ggcttatgcg ttctgtctgt tcctctatac gttctccatc attcccggta
     1261 ataaggtcat gcaaatttaa ctacgtaaaa tcgccgctgc tgtgtcctga tcggtaacca
     1321 gtgcgttgat ataaccgcct ttcattgcag cggcaattgc ttcagcttta ttttctcccc
     1381 ctgccacgcc aacccggacg ggtatggtct tcagcgcgct taaaggtaag ccaatcagtt
     1441 cgttatgtat tttgatatcc gtgacaacgt caccttttgc atcaaaaaag tagcctaaaa
     1501 tgtcgccaac cgcccctttt cggccaatca ttaactgttc gccctggctg atataaccgg
     1561 agcgaatgat tgtcgcgtcg tcttgttgac tcacggctcc aatgccgaca atcgccacat
     1621 ccgctgcttg cgcggctagc agaacatctt tgacgcaatt ttcatttttt agcgtacggg
     1681 caatgtcagc ggaggatgcc cgcaacggag ccggaataat attcacactg cacgccgcgt
     1741 taagctgccc gatacccgtc atataagaac cgacgccacc ggagagcgtg accaggcgaa
     1801 tttgctgtga cgaaataaaa ccacttaagc gttgcagcgt attcatggtt gcctcgccaa
     1861 aaccaatcgc cagcatctgt tgtggttgaa gtaaactcat caacatatgc gccgcgccta
     1921 tccctagtcg cccgccgaca tcagcatccg caagcccagg gatcacccgg acatgttgca
     1981 gcgaaaactg acgacgtaat tgagtttcat attccagaca gccttcaaag cgagaattaa
     2041 tctgtacgcg aataatgccg gactgatgcc ctttctccag caagcgcgac actttcaaac
     2101 gggtcaggcc gagacgatcg ctgatctcgc tctgggtcag cccgtcgtga tagtaaaacc
     2161 acgcgatccg cgcgacctgt tcttcttcac acattccctg ttctgaaatt gccgaatcgt
     2221 tgattgtcat aattcattct tcactttgaa catatttaaa tctttaatgc aattgttcag
     2281 ttcttgcgca tttatatctg tgatggcaac cacagtttga ctctacgagc atgaacaaac
     2341 gcaaccgtga aaatcaaaat agcataaatt gtgatctatt cgtcggaaat atgtgcaatg
     2401 tccacctaag gttatgaaca aattaaaagt agaaatacat ttgttcaaaa ctcacctgca
     2461 aaactgaacg ggggaaatat gcaaacgagt gatacccgcg cgttaccgct actttgcgcc
     2521 cgctcggttt ataaacagta ttcaggggtc aatgtcctga aaggcatcga ttttacgttg
     2581 catcaggggg aggtccacgc cctgctcggc ggcaatggtg ccggtaaatc gacgttaatg
     2641 aagattattg ccggtattac ccctgctgat agcggtacgc tggagattgg gggcaacaac
     2701 tacgccagat taacgccagt tcatgctcat cagctgggca tttatctcgt tccccaggaa
     2761 ccgctgcttt tcccaagcct gtcgataaaa gaaaacatcc tgtttgggct ggcaaaaaaa
     2821 cagctctcca tgcagaaaat gaagaacttg ctggcggcgc tgggctgcca gtttgatctg
     2881 catagtctgg caggatcgct ggatgtcgcc gatcgccaaa tggtggaaat cctccgcggg
     2941 ctgatgcgcg actcgcggat tctgatcctc gatgaaccta ccgcctcgct tacccctgcg
     3001 gaaaccgaac gcttgtttag tcgcttgcaa gagctgcttg ctactggcgt gggtattgtt
     3061 tttatctcgc ataagctgcc ggaaattcgc cagattgccg atcgaattag cgtgatgcgc
     3121 gacggaacca tcgccttaag cggcaaaacc agcgaactgt ctaccgacga cattattcag
     3181 gccatcaccc cagcggtacg ggaaaaatcg ctctctgcca gccaaaaatt atggctggag
     3241 ttacctggta accgcccaca acatgccgtc ggaacgccgg tgctgacact ggaaaatctg
     3301 actggcgaag gcttcaggaa tgtcagcctg acgctcaatg ccggagaaat tctgggcctg
     3361 gctgggctgg tgggagccgg acgcacagaa ctggccgaga cgctctatgg tctgcgtact
     3421 ttgcgtggcg gacgcattat gctgaatggt aaagagatca ataaattatc cactggagaa
     3481 cgtttactgc gcggtctggt ttatctgccg gaagatcgcc agtcatccgg actgaatctc
     3541 gatgcttcgc tggcctggaa cgtctgcgcc cttactcata accttcgtgg attctgggcg
     3601 aaaaccgcga aagataatgc caccctggaa cgttatcgtc gggcgctgaa tattaaattt
     3661 aaccaaccgg aacaagctgc acggacatta tccggtagca accagcaaaa aatcctcatt
     3721 gccaaatgcc tggaagcttc gccgcaagca ttgattgtcg atgagccgac gcgcggcgtg
     3781 gatgtctcgg cccgtaatga tatctaccag ctgttgcgca gcatcgccgc acagaatgtg
     3841 gctgtgctgt ttatctcctc tgatatggaa gagatcgaac tgatggcaga tcgtgtgtat
     3901 gtgatgcatc agggcgaaat tacccactct gcactgaccg ggcgcgatat taatgtcgag
     3961 actattatgc gcgttgcctt cggcgatagt cagcgtcagg aggcatcatg ctgaagttta
     4021 ttcagaacaa ccgtgaaatc acggcattgc tggcggtggt gctgctgttt gtattacccg
     4081 gttttctcga ccgccagtat ttaagtgtgc aaacgctgac catggtttat agcagcgcgc
     4141 aaatcctgat cctgctggca atgggcgcga cgctggtaat gcttacgcgc aatattgatg
     4201 tttcagtggg ttcgattacc ggaatgtgcg cggtgctgtt ggggatgtta ctgaacgcag
     4261 gatattcact acctgttgcc tgtgtcgcga ctttactgct tggtttgctc gcgggatttt
     4321 tcaacggtgc cctggtcgcg tggctaaaga tccctgccat tgttgccacc cttggcacgt
     4381 tagggttgta cagaggcatc atgttgctgt ggactggcgg taaatggatt gaagggttac
     4441 ccgccgaact gaaacagctc tccgccccgc tgctgcttgg catttcagca attggttggt
     4501 tgacgataat tctggtggca tttatggcct ggctgctggc gaagacggcg tttggacgca
     4561 gtttttatgc cacgggcgat aatttacagg gcgctcgtca actgggcgtt cgtacggaag
     4621 ccattcgcat tgtggcattt tcgttgaacg gctgcatggc ggcactggcg ggaattgtgt
     4681 ttgcttcgca gattggtttt atcctcaacc agaccggtac cgggctggag atgaaagcaa
     4741 ttgcagcctg cgtgctgggc ggcattagtt tgctcggtgg ttccggtgcg atcatcggtg
     4801 cggtactcgg cgcatggttc ctgacgcaga tcgatagcgt actggtgctg ttgcgcattc
     4861 cggcatggtg gaatgatttt atcgcgggtc tggttctgct ggcggtgctg gtgtttgatg
     4921 gacgcctgcg ttgtgcgctg gaacgtaatc tacggcggca aaaatatgcc cgctttatga
     4981 cgccaccgcc atccgttaaa cccgcttcgt caggtaaaaa acgggaggcc gcataatgcg
     5041 tattcgctac ggttgggaac tggctcttgc cgcactgctc gttattgaga ttgtcgcatt
     5101 tggtgcaatt aacccgcgaa tgttagatct caatatgttg ctgttcagca ccagtgactt
     5161 tatctgcatt ggcattgtcg ctctaccgct gacgatggtg attgtcagtg gcgggatcga
     5221 tatttcgttt ggttcgacca tcggcctctg cgccattgca ttgggcgtac tgtttcaaag
     5281 tggtgtgccg atgccgctgg cgatactcct gaccttactg ctcggcgcat tgtgcgggct
     5341 gatcaacgcc ggattaatta tctataccaa agttaacccg ctggtgatta cgcttggcac
     5401 gctgtatctg tttgccggaa gcgctctgct gctttccggt atggccggag cgacggggta
     5461 cgaaggtatt ggtggattcc cgatggcgtt tacagatttc gctaacctgg atgtgctggg
     5521 actccccgtt ccgctgatta tcttcctgat atgtctcctc gttttctggc tctggctgca
     5581 taaaacccat gccggacgta atgtgttttt gattgggcaa agcccgcgcg tggcgcttta
     5641 tagcgcgatt ccagttaacc gcaccttatg tgcgctctat gccatgacgg ggctggcgtc
     5701 tgcggtcgcc gctgtgctgc tggtatcgta ttttggttca gcacgttccg atctcggtgc
     5761 gtcgtttctg atgcccgcca tcaccgccgt ggtgcttggc ggggccaata tttatggtgg
     5821 ttccggttcc attatcggca ccgccattgc ggttttatta gtgggatatt tgcagcaagg
     5881 tttgcaaatg gcaggagtgc caaatcaggt gtccagcgcc ctttccggtg cgctgcttat
     5941 cgtcgttgtc gtaggtcgtt ccgttagcct gcatcgccag caaattaaag agtggctggc
     6001 gcgtcgggcc aataacccat tgccataaag gatatcttca tgacacttca tcgctttaag
     6061 aaaatcgcct tacttagcgc tcttggcatt gccgcaatct ctatgaatgt gcaggccgca
     6121 gagcgtattg catttattcc caaactggtt ggcgtgggat tttttaccag cggtggcaac
     6181 ggcgcacaac aagcgggtaa agagctgagc gttgatgtga cctacgacgg gccgacagaa
     6241 cccagtgttt ctggtcaggt acagttgatt aataacttcg tcaatcaagg ttataacgcc
     6301 attattcgtt tctgcggttt cgcctgatgg cttgtgtccg gcactgaaac gcgccatgca
     6361 acgtggtgtg agagtgctga cctgggactc tgatactaaa ccggagtgcc gctcttacta
     6421 cattaatcag ggaacgcccg cccagttggg aggtatgttg gtggatatgg cggcgcgtca
     6481 ggtgaataaa gacaaagcca aagtcgcgtt tttctactca agccccaccg ttacggacca
     6541 aaaccagtgg gtgaaagaag cgaaagcgaa aatcgccaaa gagcatccag gctgggaaat
     6601 tgtcactacg cagtttggct ataacgatgc cactaaatcg ttacaaaccg cagaaggaat
     6661 attaaaagcg tatagcgatc tcgacgccat tatcgccccc gatgccaacg ccctgcccgc
     6721 tgccgcacaa gccgcagaaa acctgaaaaa tgacaaagta gcgattgtcg gattcagtac
     6781 gccaaatgtg atgcgcccgt atgtagagcg cggcacggtg aaagaatttg gactgtggga
     6841 tgtggttcag caaggcaaaa tttcagtgta tgtcgcggat gtattattga aaaaaggatc
     6901 aatgaaaacg ggcgacaagc tggatatcca gggcgtaggt caggttgaag tctcgccaaa
     6961 cagcgttcag ggctatgact acgaagcgga tggtaatggc atcgtactgt taccggagcg
     7021 cgtgatattc aacaaagaga atatcggcaa atacgatttc tgatgtgcat tacttaaccg
     7081 gagtaagtta tggcagattt agacgatatt aaagatggta aagattttcg taccgatcaa
     7141 ccgcaacaaa atatcccttt taccctgaaa ggttgcggtg cgc
//
LOCUS       DAEBXN010000171         7036 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.171, whole genome shotgun sequence.
ACCESSION   DAEBXN010000171 DAEBXN010000000
VERSION     DAEBXN010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7036
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.171"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(439..1020)
                     /locus_tag="KJL99_003944"
     CDS             complement(439..1020)
                     /locus_tag="KJL99_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438495.1"
                     /translation="MYYPVTDYIALALIISFLFLTLFICLLCLKHERIKKETIRQKNA
                     HILEHGWNATEFSWFRYGQYNETGIYISIEKTIIITITVSGGCFKKEYSIVSHMLVTD
                     TITEATLYENGLYTRHIRLSRPVSDSKYPLPPGSQLIKNMTLRLRLQDQQEETSVTLF
                     QGKMSTDGNNYYIIKGKVSSVLLLLKMLQINHA"
     gene            complement(1067..4924)
                     /gene="sigA"
                     /locus_tag="KJL99_003945"
     CDS             complement(1067..4924)
                     /gene="sigA"
                     /locus_tag="KJL99_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HBD4438496.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTVGFISSTNIAWTNYSLFNNILVNNLKKNFTNTMQLDGKKQELSSIIKDKDLSVS
                     GGGVLTLKQDTDLGIGGLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVAGD
                     NLHKIGSGTLDVKIAQGNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSES
                     GNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGN
                     VSGNISINHIINTTQQHNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGN
                     NNCPIPFLCQKDYSAAIKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKLNL
                     ENATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGDSA
                     GESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIE
                     LAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILF
                     GVDQYKEDNLDKTTHTPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQS
                     LKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGS
                     NNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNISPVISTKEDQEH
                     TTWTLTGYKVAENTASSGAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEAGAWARI
                     MSGAGSASGGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSV
                     GAGLYASAIFDSGAYIDLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRYHV
                     TEDSWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPLIGRTGVDVGKSFSGKDW
                     KVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRILMNVGLNAEIRDNLRFGLE
                     FEKSAFGKYNVDNAINANFRYSF"
     gene            complement(5285..6289)
                     /locus_tag="KJL99_003946"
                     /pseudo
     CDS             complement(5285..6289)
                     /locus_tag="KJL99_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
     gene            6274..6651
                     /locus_tag="KJL99_003947"
                     /pseudo
     CDS             6274..6651
                     /locus_tag="KJL99_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004902302.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6690..>7036)
                     /locus_tag="KJL99_003948"
     CDS             complement(6690..>7036)
                     /locus_tag="KJL99_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBD4438497.1"
                     /translation="VPGEYSSGNSIRPRGITKVGNSELRRLLYEAAWSYRTPAKVGAW
                     LIYYRPDSVTQYSKDIAWKAQQRLCSRYRTLTAKGKKSQVAITAVARELTGFMWDIAL
                     AAQSSFSQQKQN"
BASE COUNT         1749 a         1595 c         1587 g         2105 t
ORIGIN      
        1 ctgcttccgt ggcgcatagc actgccagct gaataacaca tccccgtcaa tacggccctc
       61 gctgtacgct tactcatcac ctgctattaa tccccgagtc agtacttcgc ggcctgacgc
      121 gatcacccta ctcagtactt cagagcccgc ctccggtacc tgcctggcga tcgcccctgt
      181 gcaacaaaat aaatcaaccg ttatcaaacc acgatcgttc gtatgcgccg ccgatgacgc
      241 gcgcctgaaa aacgcgtcaa tatgagttcc ccggaagctt acgataatac gcttatgaac
      301 attacaacac gctcatgcgg aacctggatg ggggaaggaa cggtgaagct gttagcgaat
      361 gaccggcgtt tatacaaacg ccggtcatga tgaatgatat ttcagaatat acctcgggct
      421 aaaataccgg gacagagatc aggcgtgatt aatctgcagc atttttaata acagaagtac
      481 tgagcttact ttacctttga tgatgtaata gttatttccg tcggtactca tttttccctg
      541 aaataatgtc acggatgttt cttcctgctg atcctgcagg cgcagtcgta aggtcatatt
      601 tttaatgagc tgagatccgg gtggcaaggg gtatttgctg tcagatactg ggcgactgag
      661 tctgatgtgg cgcgtataca gcccgttttc gtacagggtg gcctctgtta tggtgtcagt
      721 gaccagcatg tgggagacaa tgctgtattc ttttttaaag cacccaccag aaacggtgat
      781 tgtaatgata atggtcttct cgatcgagat gtaaataccc gtttcgttat actgtccgta
      841 tcggaaccat gagaactcag ttgcattcca gccatgctcc agaatatgtg catttttttg
      901 cctgatagtt tcttttttta ttcgctcatg tttaaggcat aacaggcaga taaataatgt
      961 cagaaaaaga aagctaataa tgagagcaag agcgatatag tctgtcacag ggtaatacat
     1021 attctctccg gtagaagaag ggccgcaaac gcggcccggg ctgttatcag aaagagtaac
     1081 ggaagttggc gttgatcgcg ttatccacgt tgtatttacc aaatgccgat ttctcaaact
     1141 caagaccgaa gcgaagatta tcgcgaattt cggcgttgag accaacattc atgagaatac
     1201 gaccgtcttt ttcacctttg atacgtttct caccggacgc atcacgcagt acggtttcac
     1261 cgttggcaaa caggtcaaac tggtagccaa ggccggcgcg ggctgtgact ttccagtcct
     1321 taccggagaa ggatttaccc acatcaacac cggtacgccc aatcagcgga ttaaagtcct
     1381 tatccttcat ggtcacgctc attccgcgat cctgccagtc gaaccgttta cctgatacgg
     1441 ccccataaac cagttctgcc tgcggctcaa tccaggagtc ttctgttaca tgatagcggt
     1501 agcctgcttc agcacccaca tacaaggaat gagaactgta gtcttttgtt ccgagtccag
     1561 caaaggtcgc cgagtactca ttatcatggt gaacatactt actaatcagg tcgatatagg
     1621 caccagagtc aaatatagcg gaagcataca gacctgcccc gacggacttc gtcttgccac
     1681 tgaatgcatt actgctggca tgactgtcgg tatacgtcat agtcagacca gtgaaaaggt
     1741 caagtccatc cagctcatgt tttttatcca caccaatctg cacatgggtg tagttgtcac
     1801 tgtatccacc agaagctgaa cctgctccgc tcatgatgcg ggcccatgca ccggcctcgc
     1861 cattggtgtc acgcagatcc cccattcgtt tattcaggtt gttgacttct gtcaggaagg
     1921 ctttgtaatt accggacata tacgattttg ctgcaccgga agatgctgta ttttcagcca
     1981 ccttatatcc ggtcagggtc cacgtggtat gttcctgatc ctctttcgtg ctgatcacag
     2041 gagatatgtt gctgaaacct atggcctgag tactggcttt aaatatctct ttattgcttc
     2101 cttttggggc tgttatcagg tcaacgttta atcctgagtt attatttccc actttctgta
     2161 tatcgaccga cagactgtta ttgcttccgg ttaactgatt tttaacaatg agtttatccg
     2221 attgatttac agactggctc agatcagccc gcataatcac agcagtatta gtggcatcca
     2281 gtgtgtttac tgtcacagta tggaaacccg aattctctat gttactaaac aacaaaagac
     2341 tgtcgcgggt ttttaatgat tgcagggctg actgtccgtt tactctccat aaggtgttat
     2401 acatgctggc tgaggctgca ggagcatcaa tacctccggt ataagaagta tcaaagccac
     2461 ccagcaaacc tgtagccagc ggtgtgtgtg tggttttgtc caggttatct tctttatact
     2521 ggtcaacacc aaacagaata gatgcttttt tgtctgattt tatattacca ttgacccagg
     2581 cataattttg agcctttaac gtggcaccat cttctgtcat attatatcca tctgacatat
     2641 agattgccgg gctgtattta ttattttctg taggggtgcc tgttaatgtc agggttccgg
     2701 ccagttcaat agctttatct acatacattt ttccctgggc ggtaacagta gctcctttgt
     2761 tgataatcag gtctgtatta attaaggata atgcaccagt catagtaaca gttgatttat
     2821 caccaacagt tattttgctg ttttgagcac tcagactgcc gttgaaacta ctttcgcctg
     2881 ctgaatcccc ggagcgaagc tgttgtctga atccaaaacc ttcgccagta atgtttcttc
     2941 ctgagaacat gtcaatgtat gcagttttat ttcccagaac aatttgagag tttttagcct
     3001 ctatgtgtcc ttttacatta gcatcccggc ctatactcag ggttgcgttt tctaaattta
     3061 atttccggaa attaaattta cgactttccc agtcgggctg ggaaaaagag gctatctgat
     3121 tgttggactt atattccgta ttgtaacgtt tatttacagt gctttcctgg tcctttatgg
     3181 cagcagaata gtctttttga cataaaaaag gaattggaca gttgttattg ccttctttaa
     3241 atatggcatg ttctgtcgca tgtccttgta atgttaactg tgcattccgg acagagatat
     3301 cgtttttgat atcgactgag ccatcaaaga tcagattggc attattgtta tgttgctggg
     3361 tagtattgat aatatgattt atgcttatat tacctgatac attcccatgg tacatatatg
     3421 catcagtatt agtaagtgat aatgttgatt gcttcacgtt tgagttcgtt acggttgttc
     3481 ccgcatctgt tgctgcgatt ttctgaaatg actggtcata gccgtttaga tccagtgttc
     3541 cgccatttct ggtaaaatag atttcgccat taccgctttc gcttaaagcg tctttggcat
     3601 ttatttttac cgtaccttta cctccggcca tgtaaatttt attgaaggct ttttcagcac
     3661 taaggatgac agtcccatta cctattttaa ggttatttcc ctgtgctatt tttacatcca
     3721 gagtaccact acctatttta tgcagattat ctccggcaac gcccttaaca ttccattcaa
     3781 cggtggtatt attatcaata tctatcccgg cacctttata agacttatct tttccgtaca
     3841 ctttatatgt ctggttctta tcgaatataa gcccgccaat gccaagatcg gtatcctgct
     3901 tgagcgttaa tacccctcct cctgagacag acaggtcctt atcttttata atcgatgata
     3961 actcttgttt tttaccatcc agctgcatag tgtttgtgaa attctttttt aaattgttta
     4021 ccagaatatt attaaataac gagtagtttg tccaggctat attggtacta ctgataaaac
     4081 caactgtact ggtaacacca tggaaaaccc atttttgttg ttctttatca tatagataag
     4141 aaccagaacc actatcgcca aaaattggtg agtttttaaa aggggttctg ttggtataaa
     4201 tgtaactctt gtttttccag tccagattaa aaagacttgc gcttaacaag tcgggattat
     4261 agttttgtcc aaacagatat tgtttgccgt ttaatatcgt tgaggttgtt ccggcacctg
     4321 ctctgaagcc aattatttgt tccttaccgt tgtagtttac gccatatctt ttcatcgcat
     4381 cttcgggaga aagtgagaag tcggcactct ccgtaacccc tgttgtttcc acgacgaatt
     4441 tattcagacg atgaaccgaa aagtcgccag atgatactct gttacttgct ttatatgctg
     4501 tctgatccca tgactgtgtt gttattgcat gatgctgtgt accgttatga gtcgcagtaa
     4561 cactgtacgc acctccaatt gatgttgttg cacctttgtt ggaaacagca gaaaaatcag
     4621 gtacaggtag ttctggaaaa tgaaatatct ttccatcttt catcattaat tgaacatttg
     4681 ttgcacccgg tctgaactct cctctgtttt gagcaaggtc aagataatcg cgggtccata
     4741 tattatttat atccagcatt gctgcaaatg ctgtttcgcc ataacttcca tatattgagg
     4801 ataaaataat accgaggata actttctttc ttgatgtacc gacactaact ttccgggtca
     4861 gttcagaaac agcaactaat ccacctgtaa tatgactata tttcagtgaa taaattttat
     4921 tcatacgtga actccgatat ttctgagttg tggttatggg atagcattta attacaacgg
     4981 ggaactatca gtcatggtac gtaactgata agggtataat gataatcact gtcagttata
     5041 tttgtatgac agatgtaaca aaatgaaaaa aaagataagc acaagtgatt gattggttag
     5101 atattgatca cttggtgttt gtattttgaa ttttgcgatc aaaatttgat ctttgttgtt
     5161 tccggtcaat cctttacagg ttttctgtat tatccggggg agttaagtct gtcgatgtcc
     5221 gacagacttt tgatgatgga aacaacatgc agcgataatg tgcatgctgt tttgcaggtg
     5281 gaactcagaa ggtgaactgg aactgtgcga ccgcaccgta agtgttgtcc gtgccgatca
     5341 ggcctgcaac atcaaggtga accacatcaa acggtgagat ccccaccccg gccgtgaatg
     5401 cactgccgtc gttaccggcc agattctgac ggtaaccggc gcgcagctgt gcccatttcc
     5461 aggcattaaa ttctgtgcca atggcggcaa actgacggtt gctgtcggag gtgaaaccgc
     5521 tggccggggt cagatccaca tcaaatgcag tggtgaacat cgcgttgtgc caggagacac
     5581 cggccgtcac ctgcgggcgg atcctgaagg tttccgtgat accgttcact tcttttgtct
     5641 caatactacg ggagatcagg ttctgtgcga ccagtcccag cgtccagttg tcatccaggt
     5701 ccgtactggc accgatgtcg gcgttgatgc catttttcgt gttgtggtag cggtcgcctt
     5761 tgaaggcgct gctgtcataa tttctgacca gcacgttgta gttgaacagg tcgacgcgct
     5821 ggaatttggg ggggaagccc agagaccatt tctgcccggc cgtttccagt tccgtagcca
     5881 gagcaatacc cacatcagtg atcagtgccg tgcgggcaca ggcctgtgat gtcaggcggc
     5941 ttttgtccac ctcctgcccg gtgctgtttg ccacctcttc caggtaagtg aggtcagcat
     6001 cagagacttt accgtcgaca ctgacggtgc cgtgtgattt aaccatcagt gcggcagaga
     6061 ggcgatcacc gggaagggcc gccacagcag aaacgcccag ctgtgcggcg gcatgtgtgt
     6121 tacggaagtc ccggaggtgt tctttcaggc gtgcggcagc ctcaggaaca ccgtggttac
     6181 tttcgatggc gcggtcgaag gccttccact catcactgat ccggtcggcg ttgtcggtga
     6241 tattatccgg attggagagc tgcgcgccga cggacggaag caggatgctg ggctcatgcg
     6301 cgacgcaaaa tccacgatgt atatatcagt actaaaagcg cgacggcgga agaagctctg
     6361 aaactaatcg gtgaactgta cgccatagag cacgatatac gcgggttacc ggtgtctgaa
     6421 cgcctggcgg tcaggcaaat gcagagtaga ccgctcctga cttccctgta taagctgatg
     6481 caggagaaag aacacacgtt atcgaagaaa tgtcgtctgg gagatgcgtt ccggtatatc
     6541 aggaagcact gggctgcgtt gtgcaacttc tgtgatgatg gcctggctga ggtagataat
     6601 aatgcagcag aaagagcgct tcgtgcagtc tgtctcggga agaaaacttt ttcgaggatc
     6661 actcattccg tgtctgactg cttcagggtt taattttgct tctgctgact gaatgatgat
     6721 tgggcagcaa gtgcaatatc ccacataaat ccagtcaact cacgagccac cgccgtaatg
     6781 gctacttgtg attttttccc ttttgctgtc agagttcggt aacgagaaca caatcgttgt
     6841 tgagctttcc atgcaatatc tttggaatat tgtgttacag agtccggtcg gtaatatata
     6901 agccatgctc caacttttgc aggtgtacga taagaccagg cggcttcgta aagcagacgt
     6961 ctcagttcgc tgtttccaac ttttgttatt cctctgggac gaatactgtt tccactggaa
     7021 tattcacctg gcacca
//
LOCUS       DAEBXN010000172         6910 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.172, whole genome shotgun sequence.
ACCESSION   DAEBXN010000172 DAEBXN010000000
VERSION     DAEBXN010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6910
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.172"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..694
                     /locus_tag="KJL99_003949"
     CDS             <1..694
                     /locus_tag="KJL99_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBD4438498.1"
                     /translation="DAEAICEAASRPSMRFVQPRTETQQAIRALHRVRESLIRDKVKT
                     TNQIHGFLLEFGISLPTGDAVIKRLSLVLAEHEIPEYLSRLLVRLHTHYLYLVEQIAE
                     LESELSQSINADDTAQRIMTIPGVGPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQ
                     YSTGGKSTLLGISKRGDKNLRRLLVQCARSFMMQLERQHGKLAEWVREQLNKKHSNVV
                     ACALANKLARIA"
     gene            complement(1033..1803)
                     /locus_tag="KJL99_003950"
     CDS             complement(1033..1803)
                     /locus_tag="KJL99_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438499.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQCNPNYPGWISIYVR
                     LDAPRLATLLVNRHDGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLTEDEIRAVLDAVRDAVCSVSCRGAEDARRIRAALTTSGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(1848..2282)
                     /locus_tag="KJL99_003951"
                     /pseudo
     CDS             complement(1848..2282)
                     /locus_tag="KJL99_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
     gene            complement(2294..2515)
                     /locus_tag="KJL99_003952"
     CDS             complement(2294..2515)
                     /locus_tag="KJL99_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438500.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            complement(2516..3199)
                     /locus_tag="KJL99_003953"
     CDS             complement(2516..3199)
                     /locus_tag="KJL99_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HBD4438501.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(3263..3574)
                     /locus_tag="KJL99_003954"
     CDS             complement(3263..3574)
                     /locus_tag="KJL99_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438502.1"
                     /translation="MKYDGDGRATARFFSDKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLVSVTVYASPSPFSDERPSSRFRGITLLSKRRRLRYSTVGLTRYRK
                     R"
     gene            complement(3571..4473)
                     /locus_tag="KJL99_003955"
     CDS             complement(3571..4473)
                     /locus_tag="KJL99_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HBD4438503.1"
                     /translation="MYFPGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QITENIRSEQCPGVTAAGRGAVSTENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDARETITVHFTAKRGDWCDIWGNEDVSVWWNRLCDNVVPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGENFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVNVLWGELEWSSP
                     TDDDEQPEVTGPAWIVDNVAHYGG"
     gene            4913..5872
                     /locus_tag="KJL99_003956"
     CDS             4913..5872
                     /locus_tag="KJL99_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HBD4438504.1"
                     /translation="MLKVSCDDGSTNVKLAWFEDGEVRTSLSGNSFKEGWNPGLFNAG
                     KVYNYVVDEKKYTYDLGSTAVIGTTHVSYQYSTTNLLAIHHALLTSGLQPQDVELTVT
                     LPVTEFFDNDNQPNEERIERKKANVLREISLNKGETFKIKKVNVMPESLPAAFESLKK
                     DKVNKLERSLIIDLGGTTLDCGLILGAFEGISEIRGYSEIGTSRITHTVMNALTKAST
                     PCNYFIADELIKNRHDNEYLQTLINDVAEIKNISHVIDREVKSLAESIRQEISTFSGM
                     NRIYLTGGGAELIYPHIKQYFPNLKVNKVDEPQFALVKAMVHA"
     gene            5872..6267
                     /locus_tag="KJL99_003957"
     CDS             5872..6267
                     /locus_tag="KJL99_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HBD4438505.1"
                     /translation="MESSDPKKRKKVVAYLHPALYPQDNLTQQTIDSLPVQMRGDFYR
                     QSLICGAALYSVAPRLLTLISVFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIE
                     ASSENKSESITSKDDFEEQTRRNLSMLKK"
     gene            6450..6830
                     /locus_tag="KJL99_003958"
     CDS             6450..6830
                     /locus_tag="KJL99_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HBD4438506.1"
                     /translation="MTMATINARIDDDIKNQADEVLKLMNISQTQAIAAFYQYITEQK
                     KLPFVITSIVKTPHDLLRESTDMLAEALAVISNLQVWTEQQDGIGKAKLMEYYRRLDA
                     LYCCAKEKIGLLSDNRDAELGCVP"
BASE COUNT         1888 a         1785 c         1675 g         1562 t
ORIGIN      
        1 tgatgcagaa gccatatgtg aagcggcttc acgaccatca atgcgttttg tccagccaag
       61 aactgagacc cagcaagcaa taagggcatt acaccgggtc agagaatcgc tcatcagaga
      121 taaggtgaaa acaaccaacc agatccacgg tttcctgctg gaatttggaa tcagcctgcc
      181 gacaggggat gccgtaataa aacggttgtc tctcgtattg gctgaacacg aaatccccga
      241 atatctgagc cgccttttgg tgaggttgca tacccactac ctttaccttg ttgagcaaat
      301 agcagagctg gaatcagaat taagtcagtc aatcaacgct gatgataccg ctcagcgcat
      361 tatgacaatt cctggtgttg ggccgattac tgccagctta ctatcgtcgc aacttggtga
      421 cgggaaacag ttttcatgca gcagagattt tgcggcttcc acagggctcg ttccccggca
      481 gtatagcact ggaggaaaat cgactctgtt gggtatcagt aagcgaggtg ataaaaattt
      541 acgccggtta ctggttcagt gtgcccgctc attcatgatg cagcttgaac gccagcatgg
      601 aaaacttgct gagtgggttc gggagcaact caacaagaag cattcgaatg tggttgcctg
      661 cgctctggca aataaactgg cccgaattgc ctaggcaatc actactcagc aaaaagaata
      721 tcaggcctga tatatagatg ctgacaaaac tcaattagct tattgcaaac aataagatac
      781 aagttaccgt ctggttttgc gaagattgat tattgatgac atgaacggca aatcgacctg
      841 attaaaaacc taacagggga aaatggctca ctgaagccga ggggattttt aggattatca
      901 ggtgcggaac tcatcgaggc gcgggcatcc cttgcccaca gagacgccgg atagatttaa
      961 gcaagcccac cattaatcaa aaatcagtgt tgcaaaaacg ggggtgacca tagattttcc
     1021 ccgccatcgt catcatttcc ggatttttgc cagggcatcc gccatgctgc ggatttcggc
     1081 ccgggcttcc cggacaactt cccgcagaac accacggtca aaccaccggc ggggcggttc
     1141 atccggtata cgcagcacat cgcaggccag cccactggac aggatatgct caatacaatc
     1201 gctgaccaga tacatttcca ccgccgaaaa acgtgcgccc cggttcagga tggcatcgag
     1261 caccacgggc aggttttcat cgtcttcatc gagccagcag gggtgatcgc tttccttcac
     1321 gaccaggcga aagcggcgcg tctggcgggt cagcaacgtc tgaccgcttg tggtcagcgc
     1381 cgcccggata cgccgcgcat cttctgcccc ccggcaactg acactgcata ctgcatcgcg
     1441 caccgcatca agaactgccc tgatttcgtc ttcggtcagc cattcaccgg catacgggaa
     1501 agacacctgc gttccgtccg ccggaagtac cggcagcgac tgccactgac tgccggacag
     1561 aacaagttcc gctccggttc cggtcagttt ctgaatggcg gaggccaggt gcggcggcag
     1621 cacgccatcg tggcggttaa cgagtaacgt cgccagccgg ggcgcatcca gccgcacgta
     1681 aatgctgatc cagcccggat agttcgggtt acattggtca acatccgtct cttccggcca
     1741 gccctgcgcc agcagcaggt tacgggcgtg acgacaaatc agagcattca gcgtttcatt
     1801 cataattcat gaactccctg tatcgccgcc cacaggcggc gataacatca gtcgttttcg
     1861 tgtgtgttgt ttttcagcag gctgcgggcc tgatcaacca tcgcctgtcg gcgggtaacg
     1921 tattccggga gggggaaacc gtgatcccgg gcagcccatt cacgtttcca cagtgcctgt
     1981 tcagcgatct gagtcagggt ctccagcagt tcagcaggga cggtcacctg gcggttttcg
     2041 cgcacgttgc tgataatctc catcgccgcg gcggaagaaa taccggactc aatccgctag
     2101 tcttccggga tgtcctccag gtgcgccagg acggtcctga tttcatgatc ggacagggaa
     2161 atatccattc cgtcagcgag ggcctgaatc tcttccgggg accagataac cagcatcagc
     2221 ggcgtgtctc ccgaatactt tgctgccagc gcccggcata gcgtttcgca tgttccgtac
     2281 atactctctc ccctcaatct ttgaagcagg aatccagttc cagaatttcg gtatatgcct
     2341 ccatcaagag gcggtttgcg cgaaacaacg tctgacgcac cagacgttcc ggcagggtgt
     2401 cagcattaaa gctgtaccgc cgctccatct cattcatggt tgtacgcagg tcgcacatgg
     2461 cattggccac ctccgccact tcggcgcgaa gtttttcgtg tccggcatag ttcatttacg
     2521 cagcctccgg cataaaccag tcatcattcg cggccccctg ctgcatggcc cgctgtacgg
     2581 cagccagtcg ttgctgtccg gcacggtgat actgctcaag cagctcgata ccgatatacc
     2641 ggcgtccgga ctggagggcg gcgacgcagg ttgaaccgct gcctgcaaac gggtccagca
     2701 ctatcgcatt cgggtgtgtg aagctctcaa tcagcggttg caggctggta acgggctttt
     2761 ccgtcgggtg atggcggttg cccgaatatt tccagcccaa cacgtccggc agcgggtttt
     2821 gtggcagacg tggacggcct tttgccagga tgtaggcgca ttcgtggcga tagcccacat
     2881 atgcggcctt cgatgtgtag tttttggtga agaccaggtg accaacaacg ctgaatcccg
     2941 catttttcca ggcagacata aagcgatcga cgcggttcca gccatagaag ctcaccatca
     3001 gcgcgtcttt tttgagtacg cggtacattt cattacaggc cggttgcagc cattcatcag
     3061 ttttatcacc agcaatggta cgcccctgac ggtcacgaaa accgacgaga tatggcggat
     3121 cggtgaggat gaaatcaatg gcgttatcag gaatacgggc cataacatcg atgcagttac
     3181 cgaggacaaa acgggacata acagatcctt ctcttacaga gggcacccct gtggtgcccg
     3241 ttccccgctg cctgtgggct gttcatcgct tccggtaacg ggtcaggccg accgtggaat
     3301 accgcagccg tcgtcttttt gacagcaagg ttatgccgcg aaagcggctt gaagggcgtt
     3361 catcagaaaa gggcgacggg ctggcgtaca ctgtcaccga caccagccgg gagcggtaaa
     3421 agcgcccgtc ccgtgcccgc cggacgcggg acactgacca caggcagcgt ttatgccgcg
     3481 ccgcgtcagc cggcgcagta aaaagaggag cacggcgaca tcctttgtcg gaaaagaaac
     3541 gggcggttgc ccgcccgtct ccgtcatact tcatccgcca taatgcgcca cattgtcgac
     3601 tatccacgca ggtccggtga cttccggctg ctcatcgtca tctgtcgggg aagaccattc
     3661 aagttccccc cacagcacat tgacgagctc tccacgctca tacaactgcc agccacagaa
     3721 atcacagccc tgttcggcat accagtgctc cagcgtgcag ctgaaacggc ggcttaattc
     3781 tgcaataacg tcgctttccg gctgacacca cggagtgtcg aaatccacct gaatgaagtt
     3841 ttctccctga ctgctgatat tcaggtcata tccacaaggc cacttcacgc cataccgttc
     3901 cgtataccag tgatatgcag aaggaacacc gttcagaaca ccaccattaa agccgttcac
     3961 ttcaacatcc aggcgggtcg gcagaaccgt cagcaggtca aacggcatgg ttttttccgg
     4021 cactacgttg tcacacagac ggttccacca caccgataca tcctcgttac cccatatgtc
     4081 acaccagtca cctctttttg cggtgaaatg cacagtgatg gtttccctgg catcatccgg
     4141 taatccttcc caacgacgct ggccagtacc gctctgtaac cagagttcat gcagcatcag
     4201 gcagttttgt tcatccagca acacaccgtt ctgcaggtgt gtcagccagc gggtgaacgc
     4261 gatattttcc gtcgatacag caccacgtcc ggcagccgtc actccggggc actgttcaga
     4321 gcggatattt tcagtgatct gcagaagtcc ggcacttccg gccagaaaca gctgaatacc
     4381 ttcatttgtg gcgcggcgat aaagcggtgt gactgcaccg ctggccagtc gtttaatctc
     4441 agcgatctgt gccggctcac caggaaaata catacgattc gagcaccagt ttggcatggg
     4501 aattctcctc tcacatgcgg cctccatcag gccgtgccgc acctgtaacc gggcgaccgc
     4561 atacgaatgc aggtgcggcc ccgaagccgc agcgcgtgga ggaggaatga cgaaacgtga
     4621 ccgggtgagg aggccggcga cagcgacaca cttgcatcgg atgcagcccg gttaacgtgc
     4681 cggcacggcc tggttaaata gtatccgtct cagttcgtgg cgcaaaatga gaaaccaata
     4741 agaactcaaa atgaaccaca acaagaacat aaagagactc taaaagagca acaccgtttg
     4801 acatagactc aaaatgagtc acaataagaa acaataaaaa actcaaaaag atgcaccgac
     4861 ataaaggatg ctcaatttga gataaacaca aactcaaaac gaggcaaaaa gaatgctaaa
     4921 agtatcatgc gatgatggtt caactaacgt aaaactggcc tggtttgaag atggcgaggt
     4981 aagaaccagt ctgtcaggaa acagctttaa agaaggctgg aacccgggac tgtttaatgc
     5041 cggcaaagtt tacaattacg tcgtggatga aaaaaaatac acctatgatt tgggaagcac
     5101 tgctgtcata ggtacaacac atgtcagtta tcaatacagc accaccaatc tgcttgccat
     5161 acatcatgca ctgctgacat ccggccttca gccacaggat gtagaactga cagtaacatt
     5221 acctgtgacc gaattctttg ataatgacaa tcaaccaaat gaagaaagaa tagaaagaaa
     5281 aaaggcaaat gttctccgtg agatatctct gaataaaggg gaaacattta aaattaaaaa
     5341 agttaatgta atgcctgaat ctttgccagc tgcatttgaa tcactaaaaa aagataaagt
     5401 aaacaaactt gaacgcagcc tgattatcga tcttggagga accactctgg attgtggttt
     5461 aattctcggt gcatttgaag gcatttctga aatccgtggc tattcagaaa tcgggacatc
     5521 ccgcatcact cataccgtca tgaatgcgct gacgaaagcc tccacaccat gtaattattt
     5581 tattgctgat gagttaataa aaaacagaca cgataatgaa tatctccaga cattaataaa
     5641 tgatgtagct gaaataaaaa acatttctca tgtaatagac agagaagtga aatctctggc
     5701 agaaagcatc aggcaggaaa tttcaacatt ctcaggaatg aaccgaatct atctcactgg
     5761 cggaggagcc gagcttattt accctcacat aaagcaatat ttcccgaatc tgaaagtgaa
     5821 caaggtggat gaacctcagt ttgcattggt gaaagcaatg gtgcatgcgt aatggaaagc
     5881 agtgatccaa aaaaaagaaa gaaagttgtg gcatacctgc acccggcact ttatccgcag
     5941 gacaacctga cacaacaaac aatagactct ctcccagtac aaatgcgggg agatttctac
     6001 agacaatccc ttatttgcgg agcagctctt tattctgttg ccccccgact gttaacgcta
     6061 atcagcgttt ttttcagtga gaaaatcact gcagaaaatc tggtgaaact tatagaacaa
     6121 acaacaggat acacatccac atcaattgat ataagtgtgt taaaaaacat catagaggca
     6181 tcttcagaaa acaaatcaga gagcatcact tcaaaagatg atttcgaaga acaaaccaga
     6241 cgcaatcttt ctatgttaaa aaaataataa aagaagagca ggttcagtca attacctgct
     6301 cttcttttat acatactaaa aaatcagatt tttacgaata ttcagattat cataaaaaat
     6361 gcaacagaca ggaatgcaat aattttgcat tatctcgttg cttgtaatgc aaatatgcat
     6421 tattgtcagt gtgtgacaat aacagagaga tgacgatggc gaccattaac gcacgtattg
     6481 atgatgatat aaagaaccaa gcagatgaag tgctgaagct gatgaatatc agtcaaactc
     6541 aggctattgc agctttttat cagtatatta ctgaacaaaa gaaactgcca tttgtgatca
     6601 cctcaattgt aaaaacgccg catgatttat tacgtgaaag tacagacatg ttagctgaag
     6661 ctcttgcagt gatttccaat ctacaggtgt ggactgaaca gcaggatggg attggaaaag
     6721 caaagctaat ggagtattac cgccgcctcg acgcactgta ctgttgtgca aaagagaaaa
     6781 taggtcttct ttcagataac cgggatgctg aactaggctg tgtcccttaa tcatctgagt
     6841 tatagtaact gtctgtttac acaacatatt gaactatggc tcgctacgac cttcccgatg
     6901 aggcatggac
//
LOCUS       DAEBXN010000173         6797 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.173, whole genome shotgun sequence.
ACCESSION   DAEBXN010000173 DAEBXN010000000
VERSION     DAEBXN010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6797
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.173"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            240..2672
                     /locus_tag="KJL99_003959"
     CDS             240..2672
                     /locus_tag="KJL99_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBD4438507.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPQQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGPFPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVHKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2739..4208
                     /locus_tag="KJL99_003960"
     CDS             2739..4208
                     /locus_tag="KJL99_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HBD4438508.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4208..5869
                     /locus_tag="KJL99_003961"
     CDS             4208..5869
                     /locus_tag="KJL99_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBD4438509.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASDLLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVHLPDIYCSISESSRKIKSSEILPEGKYPVIEQSQEFISGYCNNECLLIKLNN
                     PVIVFGDHTRNIKFIDFDFVVGADGVKILSPILICERFFFWQLRSFKLDVRGYARHFK
                     VLNSCLFALPPIAEQERIVEKVSSLMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQ
                     NTAELAENWARISEHFDTLFTTEASVDALKQTILQLAVMGKLVPQDPNDEPASELLKR
                     IAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEGWEWCRIGNIVNIKSELVSPKDYL
                     NLYQVAPDIIEKGTGRVISKRTVKESGVKGPNSRFYKGQIVYSKIRPSLSKVFLAEYN
                     GLCSADMYPLDCYINPNYLLKYILSIPFLMQVKKAENRIKMPKLNSDSFYNIIVAIPP
                     YNEQQAIFDKINSIEAVCNGLISYIGIYHKTQLHLADALTDAAIN"
     gene            6090..6431
                     /gene="symE"
                     /locus_tag="KJL99_003962"
     CDS             6090..6431
                     /gene="symE"
                     /locus_tag="KJL99_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HBD4438510.1"
                     /translation="MTDTHSIAQPLEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPAAAEESELMQSLRKVCKLSARKQRQVQEF
                     IGVITGKQKVA"
     gene            complement(6478..>6797)
                     /locus_tag="KJL99_003963"
     CDS             complement(6478..>6797)
                     /locus_tag="KJL99_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438511.1"
                     /translation="LFDHLRDEVMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLR
                     LSLNMQFHELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMES
                     ALV"
BASE COUNT         2003 a         1613 c         1555 g         1626 t
ORIGIN      
        1 tttcttgttc gaaagaaacg caactataag atttttatat attttgttag agttgatatg
       61 gttcctgtaa cttctgttta tctgtctctg atatgcccag attaattcaa gcaagatata
      121 acagagatat caatgtgatc tctgctacgt actttacgtt gaaacaccgc cctaaatcat
      181 agccaggcta ttgattttat gtacaatcag aatcgtcctg ataaaagcga gaacccccca
      241 tggcggaact gaacctaagt aacctgacgg aagcagacat cattaccaaa tgcgttatgc
      301 cagccattct caatgcaggc tgggacaaca caacgcaaat cagacaggag gtcaaactcc
      361 gggacggtaa agtcatcgta cgtggtaaag ttgcggcacg cagaacggta aaatctgctg
      421 acatcgtgct gtaccacaag ccaggcattc ccttagccgt gattgaagca aaagccaaca
      481 aacatgaaat tggcaaaggg atgcaacagg gcattgaata tgcgcgcctg ctggacgttc
      541 cttttgtttt tgccaccaac ggtgatggct ttatcttccg cgatgccacc gcagccgaag
      601 gtgaatgcct tgaaaagcaa ataacgctgg atgacttccc ctcccccgct gaactctggc
      661 aaaaattctg cctctggaaa ggatacacac aagctcagct tccggtgatt actcaggact
      721 attacgacga tggcagcggc aaatcgcctc gttattatca acttcaggca atcaacaaaa
      781 ccattgaagc cgtctccaac gggcaaaacc gcgttctgct ggtcatggcg accggaacag
      841 ggaaaaccta taccgcattc cagatcatct ggcgcctgtg gaaatcaaaa aataaaaaac
      901 gcattttgtt ccttgccgat cgcaacattc tggtcgacca aaccaaaaat aatgatttcc
      961 agccatttgg tacggcaatg accaaagtca gcggacgcac cattgatccc gcttatgaaa
     1021 ttcacctcgc gctctatcag gctataactg gcccggagga agaccaaaaa gcgtttaaac
     1081 aagtcgcacc agatttcttc gatctgatcg tgatcgacga atgccatcgc ggcagcgcat
     1141 ccgaagacag cgcctggcga gagatccttg attatttcag ttccgccacc caaattggct
     1201 taaccgccac gccaaaagag acgcatgaag tctccagcac ggattacttc ggcgatccgg
     1261 tttacgtcta ctcactaaaa gaagggatcg aagacggctt cctcgctcct tataaagttg
     1321 tccgtgttga tattgatgtc gacctgcaag gctggcgccc aaccaaaggg caaactgact
     1381 taaacggcga agtgattgac gatcgtatct ataaccagaa agatttcgat cgcacgatgg
     1441 tgatcgacga acgcacagaa ctggttgcca gaaccattac cgactacctc aagcgcacca
     1501 atccgatgga taaaaccatc gtcttctgta acgacatcga tcatgcagaa cgtatgcgcc
     1561 gcgccctggt taatctcaac ccgcagcagg tgaaaaagaa cgacaaatac gtcatgaaaa
     1621 tcaccggcga tgatgaaatt ggcaaagctc agttggataa cttcatcaac ccgaaaaaac
     1681 cgtacccggt tatcgcgacc acttcagagc tgatgaccac tggtgtggat gctaaaacct
     1741 gcaagctggt agtactagac cagaacatcc agtcgatgac caaattcaag caaattatcg
     1801 gtcgtggtac acgcatcgac gaacgttacg gcaaactctg gtttaccatc ctcgacttta
     1861 aaaaagccac cgaactgttt gccgatgagc gtttcgatgg cattcccgaa aaagtcatgg
     1921 ataccacacc agaggatatc gccgatccag aatctgattt tgaagagaaa ctcgaagaaa
     1981 tcagcgaaca tgacgacgaa caggtaacag gcgttgatga accgcctgcg ccaccatacc
     2041 aggttaccga taccgatgat gtcggcccat ttccggaaga agacgagaag aaaatccgca
     2101 agtttcacgt caacggtgta gcggtgggcg ttattgcaca gcgcgttcag tattacgacg
     2161 ccgacggtaa actggttacc gaatccttta aagattacac ccgcaaaaca ctgctcaaag
     2221 aatatgcctc gctggatgac tttacccgca agtggcagga cgccgatcgc aaagaagcga
     2281 tcattcacga gctggagcaa caggggatca tctgggaagt actggcagaa gaagtcggta
     2341 aagatctcga cccgttcgac atgctttgcc acgtagtgta tggtcagccg ccgttaaccc
     2401 gcaaagagcg cgccgagaac gtgcacaagc ggaactactt cacaaaatac tctgaagcag
     2461 cgcaagccgt gctcgataat ctgctggata aatacgccga tgcgggcgta caggagatcg
     2521 aaagtattca ggtgctgaaa cttaaaccat tcgacagcat gggcacctta ccggagatta
     2581 ttaaaaccgg atttggcgac cgtaacgggt ataatcaggc gctcagcgag ctggaaaacg
     2641 aaatctacca attaccgccc cgctctgctt aacaactctc ttttgcgcct tctgcaacca
     2701 tcatgttgca gaaggccttg tttttcatgg aatacaacat gtctatcagc tcagtaatca
     2761 aatcattaca agatattatg cgcaaagatg ccggtgtgga cggcgatgcg cagcgtctcg
     2821 gtcagctctc ctggctgctg tttttgaaaa tcttcgatgc ccaggaagag gcgctggaac
     2881 tggagcagga taactatcaa tatccgatcc cacagcgtta tttgtggcgc agttgggccg
     2941 ccaacgcgca gggcattacc ggtgattccc tgctggaatt cgttaatgat gatctgtttc
     3001 cggcgttgaa aaacctcact gcgcctatcg ataaaaaccc acgcggctac gtggtgaagc
     3061 aggcgttcag cgatgcctat aactatatga aaaacggtac gctactgcgc caggtgatca
     3121 acaagctgaa cgaaattgac tttaccagcg ccagcgaacg ccatctgttt ggtgatattt
     3181 acgaacagat ccttaaagat ctgcaatctg cgggcaatgc gggcgaattc tatactccac
     3241 gcgccgtcac tcgctttatg gtggatcgcg ttgatccgaa actcggcgaa tccattatgg
     3301 acccggcctg cggtacgggc ggttttcttg cctgcgcgtt cgatcatgta aagaacaaat
     3361 acgtgaagag cgtcgccgat catcagacac tgcaacaaca gatccacggt gttgagaaaa
     3421 aacagcttcc gcacctgctg gcgaccacca atatgctgct gcacggcatt gaagtaccgg
     3481 tacaaattcg tcacgacaac accctgaata aaccgctttc ctcctgggat gagcaactgg
     3541 atgtcattgt taccaacccg ccgtttggtg gcacggaaga agacggtatt gagaagaact
     3601 ttccggcaga gatgcaaacc cgcgaaacgg cggatctgtt tctgcaactg attgtggaag
     3661 tactggcgaa aaacggtcgt gcggcggtgg tattgccgga tggcacacta tttggcgaag
     3721 gtgttaaaac caaaatcaaa aagctactta ccgaagagtg caacctgcat accatcgtgc
     3781 gtttaccgaa tggtgtgttt aatccctata ccggcattaa aaccaacctg ttgttcttta
     3841 ccaaaggtca gccaaccaaa gagatttggt tctatgaaca tccgtatccg gcgggcgtga
     3901 aaaactacag caaaaccaag ccgatgaagt ttgaagagtt tcaggcggag atcgactggt
     3961 ggggtaacga ggccgatggt tttgccagcc gcgtcgagaa tgagcaagcg tggaaagtca
     4021 gcattgatga cgtgattgcg cgcaacttca atctggatat taaaaaccca catcaggcgg
     4081 aaaccgtcag ccatgatccg gacgaactgt tagcgcagta tgcaaaacag caggcggaga
     4141 tccagacgct gcgtaatcaa ctgcgcgata ttcttggcgc tgcgctgtct ggcaaggagg
     4201 ttaactaatg agtgtggaaa agctgatcgt tgatcatatg gaaacctgga cctcggcgtt
     4261 gcaaacccgt tccaccgccg ggcgcggcag ctccggtaag attgatttgt atggtattaa
     4321 gaaattgcgt gagctgattc tggaactggc ggtgcgcggt aaactggtgc cgcaggatcc
     4381 gaatgatgaa ccggcgtcgg acctgctgaa gcgtattgcg gcggaaaaag cagagctggt
     4441 gaagcaaggc aaaattaaaa agcaaaaacc actgccagaa attagtgagg aagagaagcc
     4501 gtttgaattg ccggagggat gggagtgggt gcacctccct gatatttatt gctcaatttc
     4561 tgagtcatct agaaaaataa aatcctccga aatactacct gagggaaaat atcctgttat
     4621 cgagcaaagc caagaattta tttctggtta ttgcaacaat gaatgtctgt taataaagtt
     4681 aaataatcca gttatagttt ttggtgatca cacaaggaat attaaattta ttgattttga
     4741 ttttgttgtc ggcgctgacg gtgtgaagat tttatcacct atattaattt gtgagcgttt
     4801 ctttttttgg cagttacgat catttaaatt agatgtaaga ggatacgcga gacattttaa
     4861 agtattaaat agctgcttat ttgcattacc ccccattgca gaacaagagc gaatagttga
     4921 aaaagttagc tctttgatgt ccctctgcga ccaactagaa cagcaatccc tgaccagtct
     4981 ggacgcacat cagcaactgg ttgaaaccct gttgggaacg cttacagaca gccaaaacac
     5041 cgcggaactg gctgaaaact gggcgcgtat tagcgagcat ttcgacaccc tatttaccac
     5101 cgaagccagc gtggatgcgt taaaacagac cattctgcaa ctggccgtaa tgggcaaact
     5161 tgtgccgcag gatccgaatg acgaacccgc ctctgaactg ctcaaacgta ttgcgcagga
     5221 aaaagctcaa ttggtgaaag aagggaaaat aaaaaaacaa aaaccactgc cgccaattag
     5281 cgatgaggaa aaaccgtttg aacttccgga agggtgggaa tggtgtcgta ttggtaatat
     5341 agttaatatt aaatctgaat tagtatcacc aaaagattat ttaaatttat atcaagtagc
     5401 acctgatatt attgaaaagg gcactgggag agtgatatca aagagaacag tgaaagaatc
     5461 aggagtcaaa gggccaaata gtcgctttta taaaggacaa attgtttatt ctaaaatccg
     5521 cccgtcatta tccaaagttt ttttagcaga gtataatggt ctttgcagtg ctgatatgta
     5581 tccattagac tgttacatta atccaaatta cttattgaaa tatatccttt ccattccatt
     5641 tcttatgcaa gtcaaaaaag cagagaacag aataaaaatg ccaaaactta attctgacag
     5701 tttttacaat attattgttg cgattccccc ctataatgaa caacaagcaa tatttgataa
     5761 gataaattct attgaagcag tatgcaatgg tcttatttct tatattggga tttatcataa
     5821 aacccaactg catcttgcag acgcactcac tgacgcagcg ataaactaat tccctcgccc
     5881 tcacaaaacc aataaagcgg ctaaatagcc gctttattca catcaccaaa atttatattt
     5941 cacgctaatt ttcatcttat tttcattaat ttttagctgg actttccctg tatttactga
     6001 tgatgtatcc aggtatttag cacggtgcgg atgtgcgcca acacactcgc accgctaatc
     6061 acaatcacta tttatagaga atagtaatca tgactgacac gcattctatt gcacaaccgt
     6121 tagaagcaga agtctccccg gcaaataatc gtcaattaac cgtgagttat gcgagtcgtt
     6181 acccggatta cagccgtatt cccgccatca ccctgaaagg tcagtggctg gaagccgccg
     6241 gttttgccac cggcacggcg gtagatgtca aagtgatgga aggctgtatt gtcctcaccg
     6301 cccaacccgc cgccgccgaa gagagcgaac tgatgcagtc actacgcaag gtgtgtaagc
     6361 tgtcggcgcg taaacaaagg caggtacagg aatttattgg ggtaattaca ggtaaacaga
     6421 aagtcgcctg atacattgac ccggtagcgc cagtgctacc gggtattatt cgccgcgtta
     6481 taccaacgcg ctctccatct gtttttcaaa tgcctgacga attaatccca taatgtaagg
     6541 aagttgtgcg aggtcgctga aaccaatttc tacatcgcca ttaccccagc gcccaacatt
     6601 tgtcacatct ttggcaatac ctttcggatc gaccagttca tgaaactgca tattaagcga
     6661 caatcgcagt cggctttttt gcggcacaac atcaacaaaa ttcgtttccg ctttaaacgc
     6721 aatatatagc ttcaatactt cctgcgtgat ccctgcgtcc aggcgcataa cttcatcgcg
     6781 aagatgatcg aataaca
//
LOCUS       DAEBXN010000174         6740 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.174, whole genome shotgun sequence.
ACCESSION   DAEBXN010000174 DAEBXN010000000
VERSION     DAEBXN010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6740
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.174"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            142..396
                     /gene="bssS"
                     /locus_tag="KJL99_003964"
     CDS             142..396
                     /gene="bssS"
                     /locus_tag="KJL99_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HBD4438512.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            511..1629
                     /gene="solA"
                     /locus_tag="KJL99_003965"
     CDS             511..1629
                     /gene="solA"
                     /locus_tag="KJL99_003965"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HBD4438513.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDQKSDFDLTPFRLSRFQ"
     gene            1677..1790
                     /locus_tag="KJL99_003966"
     CDS             1677..1790
                     /locus_tag="KJL99_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HBD4438514.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            2051..2617
                     /locus_tag="KJL99_003967"
     CDS             2051..2617
                     /locus_tag="KJL99_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HBD4438515.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALCLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            2621..3196
                     /locus_tag="KJL99_003968"
     CDS             2621..3196
                     /locus_tag="KJL99_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HBD4438516.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTTKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(3238..4290)
                     /locus_tag="KJL99_003969"
     CDS             complement(3238..4290)
                     /locus_tag="KJL99_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HBD4438517.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            4515..5435
                     /gene="lpxL"
                     /locus_tag="KJL99_003970"
     CDS             4515..5435
                     /gene="lpxL"
                     /locus_tag="KJL99_003970"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HBD4438518.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            5607..5804
                     /locus_tag="KJL99_003971"
     CDS             5607..5804
                     /locus_tag="KJL99_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HBD4438519.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSIMGS"
     gene            5828..>6740
                     /locus_tag="KJL99_003972"
     CDS             5828..>6740
                     /locus_tag="KJL99_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBD4438520.1"
                     /translation="MRRAISYIRFSSERQLKGDSVRRQSKLVTDWLDKNPEFYLDSSL
                     SFKDLGKSAFSGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDIDIASELLR
                     RILRAGVDVVTLSDGEHYTRESLKDPLALIKSILIMQRAHEESLRKSERVQAAWNRKK
                     ELISEGIKVSRRCPAWLRLNDDRRTFTIIPDKVEVVKRAFDLRLQGLSFWAITRTLND
                     EGHLSLNQYTPKQKGWSDTAVKKLLRNRAVIGCFTPAGREEVQGYYPAIISESLFYRV
                     QQLNTGQYGRASVSSNPLSVNLFRGIIK"
BASE COUNT         1738 a         1614 c         1606 g         1782 t
ORIGIN      
        1 tcttaaaagc agaaatttat ctgttccccc ctttaaaaat tcggtgaata cccttactta
       61 ttggtatact gaagttgctt cagataaaaa cgcattgaac ctcgaataac gttgtctagt
      121 aacacgaatt agggggccat gatggaaaaa aataatgaag tcattcagac tcatccgctc
      181 gtagggtggg acatcagcac cgttgatagc tatgatgcgc tgatgttgcg tttgcactac
      241 cagaccccaa ataagtccga gcaggaaggg actgaagttg gtcagacgct ctggttaacc
      301 actgatgttg ccagacaatt tatttcgata ttagaagcag gaatcgccaa aattgaatcc
      361 ggtgatttcc cggtaaacga gtatcggcgt cattaacatg acatgaccat ccacccaaaa
      421 ggcacctcac cggtgccttt accatttctg ggttgtatat ctgcattcag ttaattactc
      481 tgcttaaacc atgagagcag agagaaaaaa atgaaatacg atctcatcat tattggcagc
      541 ggttccgtag gcgctgccgc cgggtattat gcaacccgcg ccggtttaaa cgtgctaatg
      601 accgacgccc atatgccacc gcatcaacac ggcagccacc acggcgatac gcgattaatt
      661 cgccatgctt atggtgaagg cgaaaagtat gtcccgttgg tcctccgcgc gcaaacgctg
      721 tgggatgaac tttcccgcca caacgaagat gatcccattt ttgtacgctc tggtgtcatt
      781 aacctcggcc cggctgactc cgcatttctc gccaacgtcg cccacagcgc cgaacagtgg
      841 caactcaacg ttgaaaagct cgatgcgcaa gggattatgg cccgctggcc agaaatacgc
      901 gtcccggaca actacatcgg cttatttgag actgattccg gttttttgcg cagcgaactg
      961 gcgattaaaa cctggatcca actggcgaag gaagcgggct gtgcgcaact gttcaactgc
     1021 ccggtcaccg caattcgtca tgacgatgat ggcgtaacta ttgaaacggt tgacggtgag
     1081 tatcaggcga aaaaagcgat tgtctgcgcg ggaacatggg taaaagacct gctcccggag
     1141 ctgcctgtcc agcccgtacg taaagtattt gcctggtatc aggccgatgg ccgctatagc
     1201 gtgaagaata aattcccggc gtttaccggt gaactgccca atggcgatca atattatggt
     1261 tttccggcag aaaacgacgc gttgaagatt ggcaaacata acggaggcca ggttatccat
     1321 tcagcggatg aacgtgttcc gtttgcggaa gtggccagtg atgggtcaga agccttcccg
     1381 ttcttgcgca atgtattgcc gggtatcggt tgctgcctgt acggcgctgc ctgcacctat
     1441 gataattcgc ctgacgaaga ttttattatc gataccctac ccggccacga taatacactg
     1501 ctcattaccg gcctgagtgg gcacggtttt aaatttgcgt cagttttagg ggaaatagct
     1561 gccgattttg cgcaagacca aaaaagcgat tttgatttga cgccattcag gctttcccgc
     1621 ttccaataat catcattacg gcctccagaa tgaggccgtt tttacaggag tatttaatgc
     1681 gtcggctgtt gcactatctc atcaataata ttcgcgagca tctgatgctt tatctttttc
     1741 tctggggatt gttggcaatt atggatctga tctatgtgtt ttatttttag ttttccgcac
     1801 tgacataaac tgacaaatat ttaatctccc tttcatttag cgtgaaaaat tgaatgagat
     1861 tattcctgct tctacagcac tcaccagaca tctctgatgc tgaacccact aaacttatga
     1921 agattctttc attattaatt caaaattatt gaaataaaaa taaatatcat tcaatttgtt
     1981 atttttgtgt tgcaattcat tctccgacac gccattttct ttctctcgca aattcgtatt
     2041 ggatgcactt atgtcattca caaatacccc tgaacgctat ggtgttatat ctgcggcctt
     2101 ccattggcta agcgcaatta ttgtctacgg catgtttgct ttaggtctgt ggatggtcac
     2161 gctcagttat tacgatggct ggtatcacaa agcacccgaa ctgcataaaa gcatcggtat
     2221 tttgttaatg atggggctag ttattcgcgt tctgtggcgt gtcatatctc caccgccggg
     2281 tccgctgccg agttattcgc ccatgactcg tcttgccgca aaagctggac atctcgcatt
     2341 gtgcctattg ctgttcgcca tcggcatcag cggctatctg atctcaactg ccgatggtaa
     2401 accgatcagc gtttttggct ggtttgacgt ccccgcgacc cttgccgacg ctggcgcaca
     2461 ggcagacttt gccggtgccc tgcatttttg gctcgcgtgg agcgtcgtag tactgtctgt
     2521 tatgcacgga tttatggccc tgaagcatca tttcatcgat aaagacgaca ctctgaagcg
     2581 catgctggga aagtcgtcat ctgactatgg agtataaaaa atgaaaaaaa gcctgcttgg
     2641 tttaaccttc gcgtccctga tgttctctgc cggttcagcg gttgccgccg attacaaaat
     2701 tgacaaagaa ggtcagcacg cctttgttaa cttccgcata cagcatcttg gctatagctg
     2761 gttatatggc acctttaaag atttcgacgg tacttttacc tttgatgaaa aaaatccggc
     2821 tgccgataaa gtgaatgtga caattaacac caccagcgtc gatactaacc acgccgaacg
     2881 cgataaacat cttcgcagtg cggattttct caataccaca aaatatccac aggcaacatt
     2941 cacctccacc agcgtgaaga aagacggtga cgaactggat attaccggcg atctgacgct
     3001 gaatggcgta accaaacccg tcacgctgga agcgaaatta attggtcagg gcgacgaccc
     3061 atggggcggt aaacgtgcag gctttgaggc cgaaggcaaa attaagctca aagacttcaa
     3121 tatcaagaca gatttaggtc cagcttctca ggaagtggat ctgattattt cagtggaagg
     3181 cgtacagcag aagtaagagt gaaatgttgc cggatggcac gcatccggca atgatattta
     3241 ttctgttgga tcaggaatac ccagtgttgt attcagacgt ccacgagact tattaaagat
     3301 cttattgcca ttttcacgtc ctgcccgacg gcgtcgctgt tcttctggcg gtaacgcgct
     3361 ttcttcgcag caaatctcac tacaacaacc tttgtatttt tccgcgcata ctggacactg
     3421 aataaacagc aggtggcagc catcattttt acagttggta tggctgtcgc acggcgcgcc
     3481 gcactggtgg caatgcgcga taatctcatc cgatatacgt tcgcccatcc gctcgtcaaa
     3541 aacaaaattt ttgccaataa aacgcaccgg caagccctgc tcgcgcgcct tacgggcgta
     3601 ttcaataatt ccgccctcga tatgccagac tttgttgaat ccgttatgtt tcatccaggc
     3661 actggccttt tcacaacgaa tgccgccggt gcagtacatg acgatttttt tatctttatg
     3721 tgcctgcatc atctcgactg cttttggcag ctgctcacgg aaggtatctg ccggaatttc
     3781 gagcgcgttt tcaaagtgcc ccacttcata ctcatagtgg ttacgcatgt cgataaatag
     3841 tgcatcggga tcgtcaagca tggcgttcac ttccgccgct tgcagatact caccaacatt
     3901 gctggcatca aagtgaggat cgtcaatacc gtcggcaaca atgcgatcgc gtaccttcat
     3961 gcgcagtacc cagaaggatt tcccgtcatc atccaacgcg atattcaggc gtaaaccctc
     4021 taaagccggg tcgaaggcat agagctgcgc gcgaaatgtt tcaacattgc tcgcaggtac
     4081 gctgatttgc gcgttaatgc cctcatgcgc cagatacact cgcccaaaaa cattcagcgc
     4141 ggtaaacagc tgatataaag cgtcacgggt cgccttagga tcggcgatgt ggaaatactt
     4201 gtaaaacgaa atggtggttc gcggttcgct ctcagccaac attttggctt ttaacgcgtc
     4261 gttggaaatg cggttgtgta acactggcat ggtgtacggt tcctgcgaga tgggaaagta
     4321 aaaatccgcg gcatgatata gcaattatcg ataattaaca tccacatatt ttacgctaca
     4381 tttgcgcatt aaaaattatt tgttatttac aagcgcggca atttcgccca gtcttcagcc
     4441 acaaattttg gttgcgggcg aaaaaatgcg acaatacata caattgcccg tataggttga
     4501 aaaacaggat tgatatgacg aatctaccca agttctccac cgcactgctt catccgcgtt
     4561 attggttaac ctggttgggt attggcgtac tttggttggt cgtgcaattg ccctacccga
     4621 ttatctaccg cctcggttgt ggattaggaa aactggcgtt acgttttatg aaacgacgcg
     4681 caaaaattgt gcatcgcaac ctggaactgt gcttcccgga aatgagcgaa caagaacgcc
     4741 gtaaaatggt ggtgaagaat ttcgaatccg ttggcatggg cctgatggaa accggtatgg
     4801 cgtggttctg gccggaccgc cgaatcgccc gctggacgga agtgatcggc atggaacaca
     4861 ttcgtgacgt gcaggcgcaa aaacgcggca tcctgttagt tggcatccat tttctgacac
     4921 tggagctggg cgcgcggcag tttggtatgc aggaaccggg gattggcgtt taccgcccga
     4981 acgataatcc actgattgac tggctacaaa cctggggccg tttgcgctca aataaatcga
     5041 tgctcgaccg caaagattta aaaggcatga ttaaagccct gaaaaaaggc gaagtggtct
     5101 ggtacgcacc agatcatgat tacggcccgc gctcaagcgt tttcgtcccg ttatttgccg
     5161 ttgagcaggc tgcgaccacg accggaacct ggatgctggc acggatgtcc ggcgcatgtc
     5221 tggtgccctt cgttccacgc cgtaagccag atggcaaagg gtatcaattg attatgctgc
     5281 caccagagtg ttctccgcca ctggatgatg ccgaaactac cgccgcgtgg atgaacaaag
     5341 tggtcgaaaa atgcatcatg atggcaccag agcagtatat gtggttacac cgtcgcttta
     5401 aaacacgccc ggaaggcgtt ccttcacgct attaaatctc ccatgccgga tgcttcagaa
     5461 tggcatccgg cattaccaca gcaaatcccc ctgatttagc gataaaagct ctctggattg
     5521 cgccccctgg aagtcgggcg cataattagt gtgtttatct tttcttctta tgttcaccgc
     5581 gcctggcgca ccaacagcgg attgctatgt caccctgtga aaatgacacc cctataaact
     5641 ggaaacgaaa cctgatcgtc gcctggctag gctgttttct taccggggcc gccttcagtc
     5701 tggtaatgcc cttcttaccc ctctacgttg agcagcttgg tgttaccggc cactccgccc
     5761 tgaatatgtg gtccggtatt gtcttcagta taatgggttc ctaaacacat cattttaggt
     5821 attgaatatg agacgggcaa tcagctacat cagattttca agtgaacgac aactgaaagg
     5881 ggactctgta cgccgacaat ctaagctggt tactgactgg cttgataaga accctgagtt
     5941 ttacttggat tcatccttga gctttaaaga tttggggaaa agtgctttta gtggtaagca
     6001 cctgaaaggt ggtctaggtg actttttaac cgctatcgag aaagggcttg ttaaagctgg
     6061 tgatacgcta ctaatcgaga gtttagaccg tctgtcacgt caggatattg atattgcttc
     6121 tgagctatta cgccgtatct taagggctgg tgtagatgta gtcacactga gcgatggaga
     6181 acattacaca cgagaaagtt taaaagaccc gttagccctg attaaaagca ttctgataat
     6241 gcagcgggcg catgaggaaa gtttgagaaa gtctgagcgt gtccaagctg catggaaccg
     6301 taaaaaagaa ctcatctccg aaggtatcaa ggtatctagg cgttgtcctg catggttaag
     6361 gctgaatgat gaccgtcgaa cattcacaat cattcctgat aaagtggaag ttgttaaacg
     6421 agcatttgac ctgagattac agggtttaag cttttgggct atcaccagaa ctttaaacga
     6481 tgaaggtcac ttgtcattga atcaatacac acccaaacaa aaggggtggt ctgatactgc
     6541 tgtaaagaaa ctgctcagaa atcgtgctgt gattggatgt ttcacacctg ctggacgtga
     6601 agaagttcag ggttactacc ccgcaataat cagtgagtca ctattctatc gagtacagca
     6661 actgaacaca gggcaatatg gtagggcttc tgtatcaagt aatccgctat cagtcaactt
     6721 gttcaggggt atcatcaaat
//
LOCUS       DAEBXN010000175         6526 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.175, whole genome shotgun sequence.
ACCESSION   DAEBXN010000175 DAEBXN010000000
VERSION     DAEBXN010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6526
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.175"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            442..1830
                     /gene="narU"
                     /locus_tag="KJL99_003973"
     CDS             442..1830
                     /gene="narU"
                     /locus_tag="KJL99_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HBD4438521.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     PVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QREAVTETAATLGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1912..5652
                     /gene="narZ"
                     /locus_tag="KJL99_003974"
                     /pseudo
     CDS             1912..5652
                     /gene="narZ"
                     /locus_tag="KJL99_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
     gene            5649..>6526
                     /gene="narH"
                     /locus_tag="KJL99_003975"
     CDS             5649..>6526
                     /gene="narH"
                     /locus_tag="KJL99_003975"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HBD4438522.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCNNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQQIDDYY
                     EPFTFDYEHLHSTPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRN
                     FEAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYD
                     ADRIEEASSTEREVDL"
BASE COUNT         1559 a         1715 c         1692 g         1560 t
ORIGIN      
        1 gaccttagat gaaataaaca atacttacta taataacgat tattacaatc aggcaataag
       61 agaggaaccg atagactttc tggacagatc gttttcctcc aattcatggc ctttttagtg
      121 aataaatcat cactaattct ataagtgtct ggtcttgttg agaatatagt taatatgtaa
      181 ccctttagat tcacgatctg aagggttgaa acatgacata cgttcaaaga ttaacccccg
      241 tttgaaggtt agcttgttag ttacatttag taacacatat ctggctactt aatccccatt
      301 ctgcccccgc ggttacgctt tcttatctat tctttcaatc agcacttcac ccaattcatc
      361 cttaattcta ataactggct attgcgcctt acctcctgtt ttgtcttttt tcattaccaa
      421 tgtgtgcata tgaggaacaa tatggcactg caaaatgaga aaaatagtcg ttatctttta
      481 cgcgactgga aaccagaaaa tccggccttc tgggaaaata aaggaaaaca tattgctcga
      541 agaaacctct ggatatcagt cagttgtcta cttcttgcct tctgtgtctg gatgctattt
      601 agcgcagtta ccgttaatct caataaaatc ggttttaatt ttactaccga tcaactcttt
      661 ttattaaccg cattaccctc cgtttctggc gcattattgc gtgttcccta ctcctttatg
      721 gtgcctatat tcggtggacg ccgatggccg gtttttagta ctgcaatcct gattattcct
      781 tgcgtctggc tcggaattgc cgtgcaaaat ccgaatactc cttttgggat atttatcgtt
      841 atcgctttgc tatgcggttt tgcaggtgca aactttgctt cgagcatggg caatatcagt
      901 ttcttctttc caaaagccaa acaagggagc gctcttggga ttaatggcgg attaggaaac
      961 ttaggtgtaa gtgtaatgca gctggttgca ccgctggtca tttttgtacc tgtatttgcc
     1021 tttctcggcg tcaatggcgt accgcaggcc gacggttcgg tgatgtcgct ggcgaatgcc
     1081 gcatggattt gggtaccgct actggcgatt gccacgatcg ccgcctggtc agggatgaat
     1141 gatatcgcca gttcacgcgc ctcaattgcc gaccagctcc ctgtcttaca acgcctgcat
     1201 ctctggctgc tgagcctgct ttaccttgcc accttcggtt cgtttatcgg tttttctgcg
     1261 ggttttgcca tgctggcaaa aacccagttc ccggatgtga atattctgcg cctggcgttc
     1321 tttggcccat ttattggtgc catcgcgcgg tcggttggtg gtgctatttc cgataaattc
     1381 ggcggcgtgc gggtgacgtt gatcaacttt atttttatgg cgattttcag tgccctgctg
     1441 ttccttacct taccgggcac aggctccggt aatttcatcg ccttttacgc cgtatttatg
     1501 gggctgtttc tgaccgcggg tctgggaagt ggttctactt tccagatgat cgccgtcatc
     1561 tttcgccaga taaccattta tcgggtgaag atgaaaggcg gtagtgatga gcaagctcag
     1621 agagaagccg tcaccgaaac ggcggcaacg ctgggcttta tctcagccat tggcgcagtg
     1681 ggcggctttt ttattccgca ggcatttggc atgtcgctca atatgaccgg ctctccggtc
     1741 ggcgcgatga aagtgttttt aatcttctac atcgtttgtg tgctgctgac ctggctggtt
     1801 tatggtcggc ggaagttcag ccaaaaataa gagatcgatg cctgatgtac aaccatgtcg
     1861 catccgacaa ggcatcagat cccaattcat tcctggagca ggagtcatgt catgagtaaa
     1921 cttttggatc gctttcgcta cttcaaacaa aagggcgaaa cctttgccga tggtcacgga
     1981 caggtgatgc atagcaaccg cgactgggag gacagctatc gccagcgttg gcagttcgac
     2041 aaaatcgtgc gttccaccca cggcgttaac tgtacaggct cctgtagctg gaaaatctac
     2101 gttaaaaatg gtctggtgac ctgggaaatc caacagaccg actacccgcg cactcgccct
     2161 gacctgccca atcatgaacc tcgcggctgc ccgcgtggcg caagttactc ctggtatctt
     2221 tacagcgcta accgcctgaa atacccgctc attcgtaaac gactgattga actgtggcgc
     2281 gaagccctca aacaacacag cgatccggta ctggcgtggg catcgattat gaatgatccg
     2341 caaaagtgcc tgagctacaa acaagtgcgt gggcgcggcg ggtttatccg ctccaactgg
     2401 caggaactaa accagctgat tgccgccgct aacgtctgga ccatcaaaac ctacggcccg
     2461 gatcgcgttg ccggtttctc gccgatcccg gcgatgtcga tggtttctta cgccgccgga
     2521 acgcgttatc tgtcgctgct tggcggcacc tgttaaagtt tctacgactg gtattgcgac
     2581 ctgccgcccg cctcgccgat gacctggggc gagcaaaccg acgtaccgga atctgccgac
     2641 tggtataact ccagctacat catcgcttgg gggtctaacg taccgcagac acgtacgccg
     2701 gacgcccact tctttaccga agtacgctac aaaggcacta aaaccatcgc cattacccct
     2761 gactactctg aagtggccaa attgtgcgac cagtggctgg caccgaaaca aggcactgat
     2821 agcgccctgg cgatggcaat ggggcatgtg attttaaaag agtttcatct cgataatccc
     2881 agcgactact ttatcaacta ctgccgccgc tacagcgaca tgccgatgct ggtaatgctg
     2941 gagcctcgcg acgatggtag ctacgttccc gggcgcatga tccgcgcatc tgacctggtg
     3001 gatggactgg gcgaaagcaa caatccgcag tggaaaaccg tagcagttaa taccgcaggt
     3061 gaattggtag tgccgaacgg ttcgattggt ttccgctggg gagaaaaagg caaatggaat
     3121 ctggaatcca ttgccgccgg tacggaaacc gaattgtcgt taaccctgct cggtcaacat
     3181 gacgctgttg caggcgtggc cttcccctac tttggcggca ttgaaaatcc gcattttcgc
     3241 agcgtaaaac acaatccggt gctggtgcgc caattgcccg ttaaaaacct gacgttagtc
     3301 gatggcaaca cctgtccggt ggtcagcgtt tatgatttgg tactggcgaa ttacggcctc
     3361 gatcgcgggc tggaagatga aaacagcgcg aaagattacg ctgaaatcaa accgtacacc
     3421 ccagcctggg gtgagcaaat taccggcgta ccgcgccagt atattgaaac catcgcccgc
     3481 gaatttgccg ataccgccca taaaacgcat gggcgctcaa tgattatcct cggcgcaggt
     3541 gttaaccact ggtatcacat ggacatgaac taccgcggga tgatcaatat gctgatcttc
     3601 tgcggttgtg tcgggcaaag cggtggcggc tgggcacact atgtcggcca ggaaaaactg
     3661 cgcccacaaa ccggctggtt gccactggcc tttgcgctcg actggaaccg accaccgcgc
     3721 cagatgaaca gcacctcgtt tttctacaat cattccagcc aatggcgcta tgaaaaagtc
     3781 tctgctcagg agttactttc accgctcgcc gatgccagta agtacagcgg tcatctgatt
     3841 gatttcaacg ttcgcgccga acgtatgggc tggctacctt ctgcgccgca gctggggcgt
     3901 aacccgctcg ggattaaagc tgaagccgac aaggccggat tatcccccac agaatttacc
     3961 gcccaggcgc tgaaatcggg cgatttacgt atggcctgcg aacaaccaga tagcggcagc
     4021 aatcatccgc gtaatttgtt tgtctggcgc tctaacctgc ttggctcctc cggcaaaggc
     4081 cacgagtata tacagaagta tctgctgggg accgaaagcg ggattcaggg cgaggaactc
     4141 ggtgccagcg acgggatcaa accggaagaa gtcgagtggc aaactgcggc gattgaaggc
     4201 aagctcgacc tgctggtgac gctcgacttc cgcatgtcca gtacctgcct gttctccgat
     4261 atcgttctgc ccaccgccac ctggtatgaa aaagacgata tgaacacctc ggatatgcat
     4321 ccatttattc atccgctttc tgcggcggtc gatccggcct gggaatcacg cagcgactgg
     4381 gaaatctaca aaggtattgc caaagcattt tcgcaagtgt gcgtgggcca tcttggcaaa
     4441 gaaaccgacg tggtattaca accactgctg catgactctc cggcagagct ctcacagccg
     4501 tgtgaagtgc tcgactggcg caaaggcgaa tgcgatctga tcccgggtaa aaccgcgccg
     4561 aatattgtgg cggtggagcg cgactaccct gctacgtatg aacgctttac ctcgctcggg
     4621 ccattgatgg acaaacttgg caatggcggt aaagggattt cgtggaatac ccaggatgaa
     4681 atcgatttcc tcggcaaact caattacacc aagcgtgatg gcccggcaca ggggcgtccg
     4741 ctgattgaca ccgccattga cgcttcagaa gtgattctgg cactggcacc ggaaaccaac
     4801 ggtcatgttg cagtcaaagc gtggcaggcg ctgggcgaga tcaccgggcg cgaacatacc
     4861 catctggcgc tgcacaaaga ggacgagaag attcgctttc gcgatattca ggcgcagccg
     4921 cgtaaaatta tctccagccc cacatggtcc ggtctggaaa gcgatcacgt atcctataac
     4981 gcgggataca ccaacgttca tgagttaatt ccgtggcgca cgctgtcggg acgccagcag
     5041 ctctatcagg atcatccgtg gatgcgtgct tttggtgaaa gcctggtggc ttatcgcccg
     5101 cctatcgaca cccgtagcgt cagtgagatg cgccagatac cgccaaacgg cttccaggaa
     5161 aaagcactta acttcctgac gccgcaccag aaatggggca ttcactcaac ctacagtgaa
     5221 aacctgctaa tgctgacgct ctctcgcggt ggaccgattg tctggatcag cgaaacagat
     5281 gcccgagaac tgaccattgt cgataacgac tgggtggaag tatttaacgc caacggcgcg
     5341 ctgactgccc gcgcggtggt cagccaacgt gtaccgccgg gcatgaccat gatgtatcac
     5401 gcccaggaac gcattatgaa tattcctggt tcggaagtaa ctggcatgcg cggcggcatt
     5461 cataactcgg tcacccgcgt ttgcccgaaa ccgacgcata tgattggcgg ttacgcgcag
     5521 ctggcctggg gctttaacta ctacggcacc gtcggttcga accgcgacga attcatcatg
     5581 atccgcaaga tgaagaacgt taactggctg gatgatgaag gtcgcgatca ggtacaggag
     5641 gcgaaaaaat gaaaatacgt tcgcaagtcg gcatggtgct taacctcgat aaatgtatcg
     5701 gctgccatac ctgttcggtg acctgtaaca acgtctggac cggacgcgaa ggcatggagt
     5761 acgcatggtt taacaacgtc gaaaccaaac cgggcattgg ttatccgaaa aactgggaag
     5821 atcaggaaga gtggcaaggc ggctgggtcc gcgatgtgaa tggcaagatc cgtccgcgtc
     5881 tgggcagcaa gatgggcgta ataaccaaaa tcttcgccaa cccggtggtg ccgcagcaga
     5941 ttgatgatta ctacgaacct ttcaccttcg actacgaaca tttgcatagc acaccggaag
     6001 gcaaacatat ccctactgct cgcccgcgtt cactgattga cggcaaacgg atggacaaag
     6061 tgatctgggg cccaaactgg gaagaactgc tgggcggcga gtttgaaaaa cgtgcccgcg
     6121 accgcaactt cgaggccatg caaaaggaga tgtacgggca gtttgaaaac accttcatga
     6181 tgtacctgcc gcgcctgtgc gaacactgcc tcaatcccag ctgcgtggcg acctgcccaa
     6241 gcggcgctat ctacaaacgc gaagaagacg gcattgtgct gattgatcag gataaatgcc
     6301 gtggctggcg tttgtgcatt agcggttgtc cgtacaaaaa aatctacttc aactggaaaa
     6361 gcggcaagtc agaaaaatgt atcttctgtt atccgcgaat tgagtcaggc caacccaccg
     6421 tgtgctcaga aacctgcgtg ggtcgcatcc ggtatctggg cgtgctgctt tacgacgccg
     6481 accgcatcga ggaagcgtcg agcaccgagc gcgaagttga cctcta
//
LOCUS       DAEBXN010000176         6335 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.177, whole genome shotgun sequence.
ACCESSION   DAEBXN010000176 DAEBXN010000000
VERSION     DAEBXN010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6335
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.177"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            191..2038
                     /gene="ldtD"
                     /locus_tag="KJL99_003976"
     CDS             191..2038
                     /gene="ldtD"
                     /locus_tag="KJL99_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HBD4438523.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPIKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLVLLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLLYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWEVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADDRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2219..2767
                     /locus_tag="KJL99_003977"
     CDS             2219..2767
                     /locus_tag="KJL99_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HBD4438524.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2794..3441
                     /gene="gloC"
                     /locus_tag="KJL99_003978"
     CDS             2794..3441
                     /gene="gloC"
                     /locus_tag="KJL99_003978"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HBD4438525.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3664..4854)
                     /gene="aspC"
                     /locus_tag="KJL99_003979"
     CDS             complement(3664..4854)
                     /gene="aspC"
                     /locus_tag="KJL99_003979"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HBD4438526.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGILEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSEIVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSSDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCESI
                     VAVL"
     gene            complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="KJL99_003980"
     CDS             complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="KJL99_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HBD4438527.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKTVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         1602 a         1551 c         1606 g         1576 t
ORIGIN      
        1 taacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt
       61 tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt
      121 ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag
      181 ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg
      241 cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc
      301 tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac
      361 ggcaataatg gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca
      421 aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt
      481 gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca
      541 gcaacagctg gcagaggtgg cgattgctgg tttccagccg cagtttaata aatgggtaga
      601 gttactgacc gatcctggtg ttaacgggat ggcacgtgac gtggtgctct ctgatgcgat
      661 gatgggctat ctccatttca ttgcaaatat tccgatcaaa ggcactcgct ggctatatag
      721 cagtaaacct tatgcgcttt caacgccgcc gctttcggtg attaaccaat ggcagctagc
      781 gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata
      841 tgcggcgatg catgaatcgt tactggtctt actcagtgac accaaaccgt ggccccaact
      901 gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga
      961 aatattgcaa cgcacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga
     1021 cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa
     1081 gccgatggac aagcaaacga cgtctcgtag taaacctgcg cctgccgttc gtgccgccta
     1141 cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggagcaga
     1201 tggtgctatt ggcccggcaa cgcgtgactg gttaaacgta acgcccgccc agcgtgctgg
     1261 cgtgttggca ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat
     1321 catggttaac attccagcct attcgctgct ctactatcag aacggcaatc aggtgctgga
     1381 ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcacttaa
     1441 caacgtagtg gtaaacccgc cgtggaacgt accaccaact ctggcacgca aagatattct
     1501 gccgaaagtg cgcaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgcgg
     1561 ctggaacagc agagaagcga ttgacccatg ggaggttgac tggtctacaa tcacggcctc
     1621 gaatttaccg tttcgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa
     1681 attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt
     1741 caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct
     1801 ggcgaatatg ctgttgcagg atgcaggatg gaatgacaaa cgtatttctg atgcgctgaa
     1861 gcagggtgat acacgttacg tcaatattcg gcagtcgatt ccggtgaatc tctactacct
     1921 gacggccttt gttggtgcag atgatcgtac ccagtatcgt acagatattt acaattatga
     1981 tctgcctgcg cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat
     2041 gaagtagttc ggggaaatca attgtcgtaa taattagcat gattgggggc gattctctgc
     2101 agcccccgtc actgctgggg ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg
     2161 cctcttgtgc gccagaagtg catataaacg ataacattga gctgtagact tgattatcat
     2221 ggacaaattc gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg cactcggtgc
     2281 cgccatcctg ccgacccctg cgtttgcaac actctctacc ccacgcccgc gcattttgac
     2341 actcaataat cttcataccg gagagtcaat caaagcggag tttttcgatg gcagaggcta
     2401 tattcaggaa gaattggcaa aacttaacca ttttttccgc gattaccgcg cgaacaaaat
     2461 aaagtccatc gacccaggat tattcgacca gttgtatcgc ctgcaagggt tgttaggcac
     2521 gcgcaaaccg gtgcaactca tttccggtta tcgttccatt gataccaaca atgaactacg
     2581 cgcccgcagc cgtggagtag cgaagaaaag ctatcacact aaaggccagg cgatggattt
     2641 ccatattgaa ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg
     2701 tggtgtagga tattacccac gtagtaactt tgtgcatatt gataccgggc cagcacggca
     2761 ctggtagtaa tcgcttaacg aaacaggggc agtatgaact atcgtattat tccggtcacc
     2821 gcattctccc agaactgttc attgatctgg tgtgaacaaa cccgcctggc cgcactggtc
     2881 gatcctggcg gcgatgcgga aaaaatcaaa caggaagttg atgacagcgg cctgacactg
     2941 atgcagatcc tgctgacgca tggtcatctg gaccacgttg gcgcagcggc ggaactggcg
     3001 caacattacg gcgtgccggt ttttggcccg gaaaaagaag atgagttctg gctgcaaggc
     3061 ttgcctgcgc aaagtcgtat gtttggtctg gaagagtgcc agccgctgac gccagatcgt
     3121 tggctgaacg aaggcgatac catcagcata gggaatgtga ctttacaggt gttacattgc
     3181 cctgggcata cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct gctgatttct
     3241 ggcgatgtta ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg cgatcataat
     3301 caactgattt cttcaatcaa agataaattg ctgccactgg gggatgacgt gacatttatt
     3361 ccgggtcacg gaccattatc tacacttggt tatgagcgcc tgcacaatcc cttcctgcaa
     3421 gacgaaatgc ccgtctggta aaggcgcata aaaaagtcca cttttaatgc gggcctggat
     3481 ttctggcaaa gtgcgctttg tttatgccgg atgcggcacg agcgccttat ccggcctaca
     3541 aaatcgtgca aattcaaaat attgcagggg acgcgtaggc ctgataagcg tagcgcatca
     3601 ggcaatgttg cgtttgtcat cagtctcagc ccgcttttca gcgggcttca ttgtttttaa
     3661 cgcttacagc actgccacaa tcgattcgca cagcggagcc atgttatctg gtgtcatccc
     3721 ggccacattt acgcgaccag aagcaacagc atatacgcca aactcttcgc gcagacgcag
     3781 cacttgttct tttgtcagac cactgaagga gaacatgccg ttctgtttga tgataaagct
     3841 gaagtcgcgg tttgcacctt tttcctgcag cgtattgacg aacaactgac gcatacgctg
     3901 aatacgctgg cgcatatcag tcagctcttg ttcccaaatc gcacgtaatg catcgctgct
     3961 caggatggtg gcaacaacag aagcgccgtg tgctggaggg ttagagtagt tagcgcgaat
     4021 cgccgctttc atttggctga atgcgcgatc aacgatttca ctgtcggcag caaccagagt
     4081 acaagcgcca acacgctcgt tgtacaggcc aaagttttta gagtaggaac tggcaacaat
     4141 cagctcttta tgcatagccg cgaaagcgcg cagtccttca gcatcttctt ccagaccacg
     4201 ggcaaaaccc tggtaagcga agtcaaacag cggtaaccag cctttctcaa cggagagttg
     4261 tgccagtgtc tgccattgtt ccagcgtagg gtcgataccg gttgggttat ggcagcagcc
     4321 atggaacagc actacgtcgc cagcctgagc ttcgttcaga ctgttaatca gtgcatcgaa
     4381 gtcaagggtg tgattttccg catcataata agcgtattca cgaacttcca gacctgcaga
     4441 gttaaagacg ctcttatggt tcggccagct tgggttgctc acccacacac gcttaacgct
     4501 ggtatttttt gccaggaagt cggcagccac gcgtagtgcg ccagtgccac ccggagtctg
     4561 tgccgtgcga gcacgtttgt cattgatcag ggcgctacct ttaccaaaca gcagttcctg
     4621 agtgcagcga ccaaattcaa ggatgccgtc aatgccgagg taatttttgg tggtttcatt
     4681 ttcgagcaga tactgttcag cctttttcac gctggtcagt accggggttt tgcccgtctc
     4741 atctttatag acaccaatcc cgaggttaat tttgccggga cgttcatcgg cacgaaacag
     4801 atcggccagg cccagaatcg ggtcggcagg agcggcggta atgttctcaa acatgacgag
     4861 gttccattat ggttacagaa gggaagtccg ctatcagggt aacgggagat ttacaaaatt
     4921 ccaactatta ctgatgaaaa cgcaggctgt ttttgcaaga cgtgagattg ctctggaagg
     4981 cataaaaaaa acaggaccaa cgtcctgttt tttcggcatt taacaaagag gtgtgctatt
     5041 agaactggta aacgataccc acagcaacgg tgtcgtctga acctacgccc agtttgttgt
     5101 cagaatcgat ctggttgatg atgtagtcaa cataggtgga catgtttttg ttgaagtagt
     5161 aggttgcgcc cacttcaaag tagttcacca gatcaacatc accgatacct tctacgtctt
     5221 tcgctttaga tttggtgtaa gcgatggacg gacgcagacc gaaatcgaac tggtattgcg
     5281 caactaacag aacgtcttgc gttttgttgg cgaagccgct ggtgtttgta aatttattag
     5341 tgatcggcgt agcgttacgg gtttcaccgt agttcgctgc caggtagatg ttgttcgcgt
     5401 cgtacttcag accagtagcc cactgttcag cttttttacc gttgccaaga ggttgagctt
     5461 cttgcaggtt ggtacggtca gctgcaccat aagcaccaac gataccaaag ccttcgtatt
     5521 cgtagctgat agaaccgcca acaccgtcgc cgttagaacg gcgtgcagtg tcacgctcgt
     5581 ttttacccag gtactgaaca gcgaagttca ggccatcaac cagaccaaag aagttggagt
     5641 tacgataggt agcaacgccg ccaacacgac caacgaagaa gtcatcgctg tatgcagtat
     5701 caccaccaaa ttctggcagc atatcggtgt aacccagtgc atcataaacc acaccgtagt
     5761 tacggccgta atcgaaagaa ccaacgtcag cgtatttaag acccgcgaat gccagacgcg
     5821 ttttgttacc agtttgagcg tcagcgcctt cagagttgtt accctggaag ttatattccc
     5881 actgaccata accggtcaga tcggaattga tttgagtttc ccctttaaaa ccaagacggg
     5941 cataggtcat gtcgccattg ccaccgtaac tgttttcacc gttacccttg gaaaaataat
     6001 gcagaccaac agttttaccg tacagatcta ctttgttgcc atctttgtta tagatttctg
     6061 cagcgtttgc agtacctgct actaacagag cagggacgat cactgccaga atattgcgct
     6121 tcatcattat ttattaccct catggttttt ttatgacacc tgccactgcc gtcaataagt
     6181 tctgtcaata aaaatttacg gaactattga tgagagtttg gtgtctttat gtgtctgcag
     6241 gcatctttcc attcaaacta acgtttcgct accgtgaaag tgctacaaag ataaagattt
     6301 agtttcaaaa agaaaaaata tgtaaccaaa agtaa
//
LOCUS       DAEBXN010000177         6321 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.178, whole genome shotgun sequence.
ACCESSION   DAEBXN010000177 DAEBXN010000000
VERSION     DAEBXN010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6321
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.178"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..378
                     /locus_tag="KJL99_003981"
     CDS             <1..378
                     /locus_tag="KJL99_003981"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438528.1"
                     /translation="IIRWLLKKRTNGSVRYCQYTSKVAMIYIEQLELIHKSGDVLYPV
                     KITRKSSGKTAFHLVPFGLNKTHDLLEVEDASEAIRLVIDERHSIRCSTLTATITNKK
                     GKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(454..918)
                     /locus_tag="KJL99_003982"
     CDS             complement(454..918)
                     /locus_tag="KJL99_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HBD4438529.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(915..1790)
                     /gene="nanK"
                     /locus_tag="KJL99_003983"
     CDS             complement(915..1790)
                     /gene="nanK"
                     /locus_tag="KJL99_003983"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HBD4438530.1"
                     /translation="MTILAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(1787..2476)
                     /locus_tag="KJL99_003984"
     CDS             complement(1787..2476)
                     /locus_tag="KJL99_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBD4438531.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(2524..4014)
                     /gene="nanT"
                     /locus_tag="KJL99_003985"
     CDS             complement(2524..4014)
                     /gene="nanT"
                     /locus_tag="KJL99_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HBD4438532.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(4124..5017)
                     /gene="nanA"
                     /locus_tag="KJL99_003986"
     CDS             complement(4124..5017)
                     /gene="nanA"
                     /locus_tag="KJL99_003986"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HBD4438533.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(5139..5930)
                     /gene="nanR"
                     /locus_tag="KJL99_003987"
     CDS             complement(5139..5930)
                     /gene="nanR"
                     /locus_tag="KJL99_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HBD4438534.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT         1609 a         1709 c         1565 g         1438 t
ORIGIN      
        1 atcatcagat ggctgcttaa aaaaagaaca aatggtagtg tccgctattg ccagtacacc
       61 tcaaaagttg ccatgattta cattgaacag cttgaattaa tccataaaag tggtgatgtg
      121 ttgtatccag taaaaataac cagaaaatca tcaggaaaaa ctgccttcca ccttgttcca
      181 ttcggcttaa ataagactca cgaccttcta gaggtggaag atgcttcaga agcaattcgg
      241 ctggtgattg atgaacgtca ttccattcgc tgcagtacac ttacagcaac tattacgaat
      301 aaaaagggca agcgaattaa gcggacaggt atatacagta tcaagggtgt taacataaaa
      361 gaatacaacg tcaggtaatg gcacagagcg gtccgtcagt tcaggtttgg ctctgtgcca
      421 aagatgggtc aggtcacacc tgatctaata aatttaagcc attaaatcag ccttaacctt
      481 caccaccacc tttttaatct cacccggctc gccaacaacg catcccggct tatgcggctc
      541 tcctggcata aacacggcga acattcccgg tttcaggatg atggtttgct cgttttcaat
      601 ggcgctacaa agctggtaat catcttcatg gtggaactct tcacattggc gcgcagtgcc
      661 tgccatgcca aacagaatcc gttcctcacc gtttaataac agctggatat caatgtattg
      721 ctcgtgcaat tccgcttttt tctcgacggg cgattgagtg ttaaacgtca tgacattcat
      781 aaaaatattg tcgccctgta attcgtaacg acccggcgct ttttcttgcg gtctggcagc
      841 taaggccagc gttaacgcgt cctgtaacgc aggatgtaac ccagcagacg gtaatgactg
      901 tacttcaccc atcatcataa tttttctccc tgggccaaca gcgcagcccc aagtaaacct
      961 gcatcatggc ggtaatgcgc cgccagtaaa tcaacatgaa atgccgctgg ctcctgcgcc
     1021 agatacattt ccaccaacgc cagataccct tctgccagac caacgctgcc accaaccacc
     1081 acgcactggc aatcagttgt ggctttaata tcagcgatca gccttgcaag cgtacgtgcg
     1141 gagcgatgaa tcagttgctg cgcctgctca tcgccctgcc cggcgcgtgt gaaaatagtt
     1201 ctcgcatccg cgccagccag ctcgccctgc gctgccgccg caatgccgcg accagaagca
     1261 attgcttcca cgcaacctgt gcgtccacag ccgcagactg ggccgtgtgg atcggcaagc
     1321 gtatgcccga tgtgccccgc cagaccgcca gggccggtaa gcagtttgcc gccgctcact
     1381 acaccgccgc cgacgccagt ggaaacggtg ataaagacca tatcggttat atcgccttcc
     1441 agcgcctgat actctgccca tgctgcggcc tgcgcgtcgt taatggcaat ggtcggcaaa
     1501 ttggtaagtt gttccagcgt tttgactaac ggaaagtgta gcaatccacc gagattatgc
     1561 ggattcagcg ccagcaaact gccgtcgcga ataattccgg ttgaagcgat ggcaacccgc
     1621 tgcgcatgag cttgcaacgg agagactaat gcggataagg catcacgcaa ggcttctggt
     1681 gtctggctgg ctggcgtagg aagttcacga cgatcgcgga tctgcccgtc agcgccaatc
     1741 agcgcggcgg caagtttagt accgccgata tcaatcgcca gtatggtcat agcaccgcct
     1801 ttttcatcgc tgtgttgtac cactgacaaa tgtgttcaag acgcgtaatg gcagaaccga
     1861 ccgtcaccgc ccacgcgccg tggcgcatcg catctgccgc ctgagcaggc gtgttgtaac
     1921 gcccttcggc aatcacccga catccggctt cgctcaacgt tttcaccagc gccagatccg
     1981 gctcttctgg cgtttcaggc gtggtatagc cagaaagcgt agtgccaata atttcggctc
     2041 ccagcttttg gcatgccagg ccgtcttccg gcgttgagca gtcggtcatc gccagtaaac
     2101 cgtgatgatg gatacgtgcc agcagcgttt caacaggcac cggacgcggg cggtcggtgc
     2161 cgtcaatggc gataatgtcc gcgcccgcct gcgccagcgc atcgacatct tcaatatagg
     2221 ccgtgatgcg taccggagaa tcctccagat cgcgtttcac aattccaata atcggcacgc
     2281 tcaccaccgc acgcgtggct tgcagatttg ccacaccttc gatgcgaatg gcaaccgcgc
     2341 ccgcctgctc tgccgctaat gccatggctg cgacgatttc gggtttatcg agcgggctgt
     2401 caggaaccgg ctggcaggag acaatcaggc caccgttagc agcgattttt tgatccagtt
     2461 gtgcaagtaa cgacatacat cttcccttag cgaaaggccc ggtacataga ccgggcaaca
     2521 ggattaactt ttggttttga ctaaatcgtt tttggcgctg ccaaatggca cggcaccgct
     2581 aaatggtttg ccgtcgatag cgtcatgagt acgcaatgct tccgggcgca gccaacgctg
     2641 aacgcgggaa ggcatatcca gaccaatcag caggatcacc acaaatgtca ggctgaacga
     2701 gagcgatgcc agcgcagtac ccagatccag acgttgagcg atcaacgcgc cgatgattgg
     2761 ggccagtgcg ccacccaatg cgccaacgtt gtaggtaaag cccaggcccg ctgcacgctg
     2821 gtcggtatcg aaataaccgc caatcagttt cggtaagatc ccggcgatcc cttgtccaag
     2881 catttgctgg aagaacagta acagaccgag cacccagacg tttgcgccgc caatcgcaaa
     2941 taccggaata atcagcagct gcgaggccag caggctacaa acgtacgctt tgcgggttcc
     3001 cagccagtca ccgaggaagc cacctacgca gcatcccacg gccgcgccaa agccactaaa
     3061 gaacagcaca ttggcgacag tatgcgggtt ataagccaga tcggttttca gatacgttgg
     3121 cagcagcgcc tgaatcggcc atgagtagag gaaagcaaac aacacgacca ccatcagcat
     3181 tacgcccgtt ggccagcgtt tacccgtact ttgcaccata aagctgataa agattgcggc
     3241 gcataacagc ccgagaacag cgacgattgc agcattttgc aggttaccgg cgaagcagaa
     3301 ccacagcgca gtagccgccg ccagtgtcat tacgatattg gcaatgcgat gttcaccacg
     3361 gtagagaata tccaccattg tgcgtactgg tgctttacct gcgtgtttct ctttccagtc
     3421 ttccgcttcc gggatgtttt tacgcagcca gagagcaaag atgattggca aaatgccgat
     3481 aaagaacagc gcacgccaac cccagaccgg aaccaccagg ctatagacct gagcggcaac
     3541 gacggccccc acagagaagc ctgaaatcaa aaaaccactg gctttgttac gcagatgttt
     3601 tggccagctt tcaatgacat aggtggcgct ggaaccgtat tcacccgcca tccccatgcc
     3661 gatgaccaga cgagcgataa acatggtgat gtagcctggc gcaaagccgc aggccagcgt
     3721 cccggccgag aagagaacga tgctggtgac cattgccaga cgacgcccgt agcggtcacc
     3781 catagcgccg agcatcaggc cgccgaacca gcgagagata aaggctgcgg agatcagact
     3841 tgccgcctgc accgtcgtca gcccgaattc accctgtact tcggtgagta ccagggcgat
     3901 taaaacgaaa tcaaaaccgt caagcagata tcccaaccag gcagcggaaa atgcgcgcca
     3961 ttgtgcacgg ttgagatggc gataccacgg gatattctgg gttgtagtac tcattgtgag
     4021 tctcccgcgg tgggcgatgc ccacacgctt tggtatgaaa attgtagggt acagatgcgt
     4081 ttatttcccc tcacccggta ggggcgagcg aggggaaaac aactcacccg cgctcttgca
     4141 tcaactgctg ggccagcgcc ttcagttctg gcagatattt ttcatctacc ggtccaaacg
     4201 gtttgcggca cagcggcaca gaaacgacat ccatataatg caggacagtt ttcaggccgc
     4261 ggaatacgcc cgttttgatc agtaaatcaa tgactttgtt gcactcagtt tgcagtttct
     4321 gcgcggtctg gatatcgcct tctttcagcg ccttcacgat cccctgatat cgccagccca
     4381 tgatgttgta agtactgccg ataccaccat cagcgcctgc cagcaagccg gaagcgaaga
     4441 tttcgtcgta accgttatag agcaccagat caggatgttc acgacggatc tgttccatct
     4501 gatagagatc gccagaagtc tgtttcagcg caccgacgcc aggcaacgta accagggtgt
     4561 tgatctgatc cagggtcagt tttaccccac tcagggctgg aatgttgtac accaccatcg
     4621 gcaaaccatc cgctgaatca ataattgccc gatagtgatc gcagtgttct tcaaagctga
     4681 aaggatagta gaacggcgtg acggcggaga cggcatcgaa gccataacgt ttagccgatg
     4741 ccgcaagttg ttggctttcg gcggtgctga cgcaaccgac gtgggcgatg agtttaatct
     4801 tacctttcgc ctcttcggcg acgatttcca gtacctgttc acgctcggaa aggctttgta
     4861 caaaggcttc gccggtcgaa ccacccacgt ataaaccgtc gatgccctgc tgaatattga
     4921 actgaaccag gcggcgcaga ctcgctttat ccagtgcttg ttgttgatca aaaggagtca
     4981 ggagtgcagc cattacgcca cgtaaattcg ttgccataaa tacctctgaa gtgatgcttg
     5041 tctgataaac gatatacctt tatacctgtt ataccagatc aattaagcaa caccccatac
     5101 agaaagctta taatgcgatc tgcttcacta aagtggcatt atttcttttt gttggtggtc
     5161 tgaccgaaag cgtgccaggt agcagagacg ctgttgagat gcgattgcaa cgcacgatcg
     5221 gcttcgtcag gatcatgacg gcggatcgca tcaacgatcg caatatgctg ttgataacta
     5281 acgttgttat gttcgtgcag tgcctgatcg gtaaccgttg ggcgtgcggc aataagccag
     5341 tcgagcaggg caacgtggat cgccatgaag attgggttac cggggatctc cgccagcacg
     5401 cggtggaaat caacgtctga acgaatgaat gccgcgttgt tatccagcga ctgactgttg
     5461 atttccagtg cttttgccag caaatcgatt tgctcatcgg tggcgtgttc agccgcatag
     5521 cgcaccaggc tggattcaaa gaacagacgt aattgttcga aatgggcaat cccaccagga
     5581 tgagaaagga aatctttcgc catgccggaa agctcgccga tgatagtgtc cgcagaaggt
     5641 cgagagacac gagcgcgttc gccgttgttt atttgcacca gacctttgcg ttttaacgct
     5701 gccagcgctt cacgcacaga aggacgcccg acgttaaaga acgccatcag ttcgcgttca
     5761 gacggtaatt gttcaccttc gccaaattca cgacggcgga tcatctgttc cagctcttct
     5821 tccaccattt cggagagttt tttacgcgcc agcgggcggc tacgcaagtt gcgaccaatt
     5881 gcaggtgaag aatcttcggt ttgcgaatca aatgcgttca taaggcccat tctgtaaggt
     5941 cagtgtgatt aacatcatca gtgacatcct atcacaggat tgaaagtagg ggaaaatggc
     6001 aggtttttct ctttgtgcct catcattacc ataattaacg gaataattaa ctattgcgaa
     6061 aaattaatgt aacgcagata aacacatccc gattgaatta ttcataagac tattcacaag
     6121 cattatgaat attatgaatg tgttcttaca aaataatcat aatcgcatat tttttaatga
     6181 aaaatcacct cacctacaat taaaaacacg acatccgcac cataaataga cttgcaaaaa
     6241 atataacatc attgttttca atctgccatt tatgggattg accgttttct tttgacacgg
     6301 agttcaacaa tgggtaatgc t
//
LOCUS       DAEBXN010000178         6263 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.179, whole genome shotgun sequence.
ACCESSION   DAEBXN010000178 DAEBXN010000000
VERSION     DAEBXN010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6263
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.179"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(231..731)
                     /locus_tag="KJL99_003988"
     CDS             complement(231..731)
                     /locus_tag="KJL99_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBD4438535.1"
                     /translation="MSVVAPAVYVGTWHKYNCGSIAGRWFDLTTFDDERDFFAACRAL
                     HQDEADPELMFQDYEGFPGNMASECHINWAWVEGFRRARDEGCEEAYRLWVDDTGETD
                     FDTFRDAWWGEADSEEAFAVEFASDTGLLADVPETVALYFDYEAYARDLFLDSFTFID
                     GHVFRR"
     gene            complement(1193..1789)
                     /locus_tag="KJL99_003989"
     CDS             complement(1193..1789)
                     /locus_tag="KJL99_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438536.1"
                     /translation="MMKSDEKYQVPAWMRPLLPLLCNTGGNDPEELLNDTETTASANV
                     VRYVLIVAVRSQVDLLQLLYRKGLLRTEIPGGFSPEEAQALLDNLVRSHISKALSGER
                     MAARDRNADLAWIGQQLVDAAWFVRATLEAHGMSVGNESPPAPPETMPDIQTRELVML
                     IKRLASSLKAVKPDSCVVREAQDWLRDRKLVDITDILR"
     gene            complement(1786..2505)
                     /locus_tag="KJL99_003990"
     CDS             complement(1786..2505)
                     /locus_tag="KJL99_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HBD4438537.1"
                     /translation="MSARSRALIPLSAEQQAAMQAVAVTEQRRRQGRTLSAWPYATAF
                     FRCLNGSRRISLTDLRFFAPALTKEEFHGNRLLWLAAVDKLIESFGEVCVLPLPSDAG
                     HRLFPSVPFREGERRRQKTTLTEQKYSRQREREAERRELEYQTCFAQAQIDLAFHTPA
                     TVGSWLSRWSGVVEEHDLETIFWGWCGRFPSLSSFDRFFWQEEPLWRLIFEAGEAGRG
                     APVQVRALEQWMIPNKLENVI"
     gene            complement(2502..2936)
                     /gene="psiB"
                     /locus_tag="KJL99_003991"
     CDS             complement(2502..2936)
                     /gene="psiB"
                     /locus_tag="KJL99_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HBD4438538.1"
                     /translation="MKTELTLNALQSMNAQEYEEIRAAGSDMRRNLTHEVMREVDAPA
                     NWMMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQLWMLVLVNGGGQPFAVV
                     QVQHIFTPVAISHTLALAATLDAQGYSVNDIIHILMVEGGQA"
     gene            complement(2989..4947)
                     /locus_tag="KJL99_003992"
     CDS             complement(2989..4947)
                     /locus_tag="KJL99_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBD4438539.1"
                     /translation="MPVTKCEPETTRKASRKYAKTQETVLSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSAESVSELAESIKGVGLLQNLVVHTLPGDRYGVAAGGRRLAALNMLA
                     ERGIIPADWPVRVKVIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPDVRVIRNLITESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEFLREAEGWEWCAGRMEPVGECREDAGTYRCLPEPEAVLTEAEE
                     ERLNELMARYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEMRAGGGVVVSWRYGNV
                     CVQRGVQLRSEDDATDDADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLALLAWTLCLNVFDSGAYSKPAQISLECKHYSLTSDAPSGKEGAAFLALMAEKARL
                     AALLPEGWSRDMTTFLSLSQEVLLSLLSFCTACSLNGVQTRECGRTSRSPLDSLDSAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNDAGLSGAARDAEKMKKGDAAEHAEFHMKD
                     NRWVPGWMCSPRPQTDATERADNLADAA"
     gene            complement(5006..5239)
                     /locus_tag="KJL99_003993"
     CDS             complement(5006..5239)
                     /locus_tag="KJL99_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HBD4438540.1"
                     /translation="MSEYFRILQGLPDGSFTREQAEAVAAQYRNVFIEDDQGTHFRLV
                     VRQDGTLIWRSWNFEDCAGYWMNQYIRDFGILK"
     gene            complement(5297..5824)
                     /locus_tag="KJL99_003994"
     CDS             complement(5297..5824)
                     /locus_tag="KJL99_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBD4438541.1"
                     /translation="MSARGINKVILVGRLGNDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTVQMLGRAPQQNAQAQPKPQQNGQPQSADATKKGGAKTKGRGRKAAQPEPQPQ
                     PPEGEDYGFSDDIPF"
BASE COUNT         1316 a         1962 c         1689 g         1296 t
ORIGIN      
        1 cggagtcggg cctgacgacc ggatgccgcc aggcagccgg gagccgaagg tgtccccgcc
       61 tccggaaatc agaacaacag aaacgacata acagaaaaat gcctcacacc atgacgggca
      121 ggaagggcag cgccgcaggc gcggccctcc tgcatttcag gcaggcgtaa gcctgccagg
      181 gttttccggc ggcctggcag gccatacccc cgccggagcg ggggagtaaa tcaccgacgg
      241 aacacatgac cgtcaataaa ggtgaaggag tccaggaata aatcccgcgc atacgcctca
      301 tagtcaaaat agagcgccac cgtctccggc acgtcagcca gcaggccggt atcactggcg
      361 aactcaaccg caaaagcctc ctcactgtca gcctcgcccc accaggcatc gcggaaggtg
      421 tcaaaatccg tctcaccggt atcatccacc cagagacgat aagcctcttc gcagccttca
      481 tcccgtgccc ggcggaagcc ttcaacccag gcccagttga tatgacattc agaggccata
      541 ttccccggga atccctcata atcctgaaac atcagttcag gatcggcttc atcctggtga
      601 agagcacggc aggcggcgaa aaagtcgcgc tcatcatcaa acgtggtcag gtcaaaccag
      661 cgtccggcga tgcttccaca gttgtatttg tgccaggttc caacgtatac agcaggtgca
      721 acaacagaca ttcccgaatc ctccatagtc acatgttctg cgacaccggg cggtgccgtg
      781 cctccataaa agccgggcga taaggcgggg atgcaggggc gcaagggaag ccgcagcctg
      841 acgcacacgg ctgacgggag ttgcagggtg aggccgcgaa acctgagcgg acgaacgacc
      901 ggaaaatcac ggcagacggg aaggagggac cgggtgaccg cagcggcgaa gcgtaccctt
      961 gcatccctgc cagcccggct aagatggagg cacggcacag cccgcacacg acgctgaggc
     1021 cacagagctg aaaacccaca aggccatgcc ccgcatggcc gggtaaaagc ggagccgccg
     1081 gcgacttcct tattccggcg agtgcagagg acacgccgca tgacctccag accacccctg
     1141 atatgaaacg atcatgatcg gcgtgtccct gcgtaaaata tacatgcctg gttcaccgga
     1201 gaatatctgt gatatccaca agttttctgt cgcgcagcca gtcctgcgct tcacgcacca
     1261 cacaactgtc cggtttcacc gctttcagcg atgatgccag tcgcttgatc aacatcacca
     1321 gttcccgtgt ctgtatgtcc ggcattgtct ccggcggagc agggggactc tcatttccga
     1381 cgctcatccc atgcgcttcc agtgtggcac ggacaaacca ggcggcatcg accagttgct
     1441 gtccaatcca ggccagatcg gcatttctgt cacgggctgc cattcgctcg cccgacagcg
     1501 ccttgctgat atggctgcgc accagattat ccaggagtgc ctgcgcttct tccggtgaaa
     1561 agccacctgg tatctctgtg cgcaacagcc ctttcctgta cagaagctgc agcagatcaa
     1621 cctgcgaccg cacagcaact atcagtacat aacggacaac attcgcactg gcagtggttt
     1681 ctgtatcatt cagcagttct tccggatcgt tccccccggt gttgcagagc aacggcaaca
     1741 gaggtcgcat ccacgcggga acctggtatt tttcgtctga tttcatcata ttacgttctc
     1801 cagcttgttc gggatcatcc actgctcaag tgcacgtacc tgtaccggtg caccacgacc
     1861 ggcctcaccg gcttcaaaaa tcagccgcca gagtggttcc tcctgccaga aaaaccggtc
     1921 aaatgatgac agtgatggaa aacgcccgca ccacccccag aaaatcgttt ccagatcatg
     1981 ctcctcaaca acaccagacc agcgggacaa ccagcttccg accgtggcgg gagtatgaaa
     2041 cgcaaggtca atctgcgcct gagcaaaaca tgtctggtat tccagttccc gtcgttctgc
     2101 ctcacgttcc cgctggcggc tgtatttctg ctctgtcagc gtggttttct gacgacgacg
     2161 ctcaccttca cgaaaaggca cggacgggaa cagacggtgc cccgcatcgg atggcagggg
     2221 aagaacacag acttccccaa aactttcaat cagtttatcc acggcagcca gccacaggag
     2281 acggttgcca tgaaattcct ccttcgtcag cgcaggggca aaaaagcgga gatcggtcag
     2341 cgaaatccgg cgactgccat tcaggcagcg aaagaaagcg gtggcataag gccatgctga
     2401 aagtgtgcgt ccctgacgac gacgttgttc tgtgacagcc accgcctgca tggcggcctg
     2461 ttgttctgcg ctgaggggga tcagtgcccg tgaacgtgcg ctcatgcctg acctccttct
     2521 accatcagga tatggatgat gtcgttaaca ctgtatccct gcgcatccag tgtcgcggca
     2581 agcgccagcg tgtgactgat cgcgacaggc gtgaagatat gttgcacctg aacgacggcg
     2641 aaaggctgtc caccaccatt caccagaacc agcatccaga gctgagagac gtctcccggc
     2701 gaacataatg ccaggtggaa acgttcgtgg gctggcgtga aacgaacctg gacggggaaa
     2761 aagcccccga actcgctgcc atactctccg ttcatcatcc agttagccgg tgcgtccact
     2821 tcacgcatca cctcgtgagt gagattacgg cgcatatcgc ttcccgcagc acggatttct
     2881 tcatattcct gggcattcat ggactgcagg gcattcagtg tcagttcagt tttcatcacg
     2941 gtatctccct ctgccgcccc gtctccggcg gggcggtgtg gttgttcatc aggcggcatc
     3001 agccaggtta tcggcgcgtt cagtggcatc cgtctgtgga cgtggtgaac acatccagcc
     3061 aggcacccag cggttgtctt tcatatggaa ctctgcatgt tcagcggcat cgcctttctt
     3121 catcttctct gcgtcccgtg cggcaccgga cagtccggca tcattcaggg ctgcgataat
     3181 ctgcggcttt ttcaggtgtc cgaagaagtt tgctttcgtc ggctgccacc agtcgcgcat
     3241 gtgaaaaccg atggcgctat ccagtgagtc aagcggactg cgtgacgtgc gaccacactc
     3301 gcgggtctgg acaccgttaa ggctgcatgc ggtgcagaaa ctgagcaggg ataacagcac
     3361 ctcctgactg agtgacagga acgtcgtcat gtcccgtgac catccctccg gtaacagggc
     3421 cgcaagacgg gctttttctg ccatcagcgc caggaatgcg gcaccttcct tccctgacgg
     3481 cgcatcgctg gtcagcgaat aatgtttaca ttccaggctg atttgtgctg gtttactgta
     3541 cgctccgctg tcaaacacat tcaggcagag cgtccatgcc aacagtgcaa gagatttgtc
     3601 cggttgctgc atcagtgctg cctggactgc cagcgtgcgt tcggaagaca ttttcgtcag
     3661 caacggcaga ctgatttcct ccactgacgc tttctcctgc acctgttccg tgcggtcagc
     3721 gtcatcagtc gcgtcatctt cactgcgcaa ctgcacacca cgctggacac atacgtttcc
     3781 ataacgccag gacaccacca ctccacctcc ggcacgcatc tccggcgtcc acgctctgac
     3841 cttcgccatg cagcgcatca gcttcatttc tgcttccagc aggtcagatt cctcacactg
     3901 gttttccagc gcgtcgtaac gtgccatcag ttcgttcaga cgttcttctt ccgcctcggt
     3961 cagcaccgct tccggctccg gcagacagcg gtatgttccg gcatcctcac ggcactcacc
     4021 gacaggctcc atgcgtccgg cgcaccattc ccagccttcg gcttcccgaa ggaactctgc
     4081 gacagcctgg agtttttcca gcagggcggc atcgagcgcc acgcagtcca catagccacc
     4141 ctcgtcgtcg ctgaacaaat cggtgcgcag ttcgtctggc gagaaggcat cagcccccac
     4201 gaagcggaat ttactgttcc ccgccaccgc cacttcactt tccgtaatca gattgcggat
     4261 aacccgcaca tccggtttac cgccccatcc cgactgacag gcagcttcaa acacctgcac
     4321 ctgacgcgcg gtgtcgttct ccagcgccag cgcctgacag tgttcggtgg tgatgcggtc
     4381 ttctgccagc gcatcgagga tgacaggcgc aaggtcagcc agtttcagca ttcgctgaac
     4441 gtggcggggt gaatagccca gcaaatcacc gatttgtgca ggtgttttgc cttcctgcgc
     4501 cattgcgcgg aatccggcaa tctgttcagc agggtgcata tcccgacgat gaccgttttc
     4561 agtcattgat gccgccgtcg ccagctcctg cggaatgacc ttcacacgga caggccagtc
     4621 agccggaatg atgccacgct ctgccagcat gttgagtgca gccagtcggc gaccacctgc
     4681 ggcgacaccg taacggtcac caggcagggt atgcacgacc agattctgca acaggccaac
     4741 gcccttaatg gactccgcca gttcgctgac ggactccgca gaatacggca ccgtgcgcac
     4801 attcagcggt gatttaatca gcgaggccag cggcacgctc acttcctccg tctgcgccag
     4861 cagggcagac aggactgttt cctgcgtttt tgcatattta cggcttgctt tgcgggtggt
     4921 ttctggttca cacttcgtta caggcataat catcccctta taattgctta tgtcctgccg
     4981 gacgttcgcg cgtccggcac ctctcttact taagaatccc gaaatccctg atgtactggt
     5041 tcatccagta cccggcacag tcctcaaaat tccaggagcg ccagatcaac gtgccatcct
     5101 gacggacaac caggcgaaaa tgcgttccct gatcatcctc gataaagacg ttccggtact
     5161 gtgcggcaac ggcttccgcc tgttcgcggg taaaggagcc gtccggcagt ccctgaagta
     5221 ttctgaaata ttcgctcatc ctcatatctc cggtgatgcc ccgcacaggg cggggcggtt
     5281 gtcacagtca gcccgatcag aacgggatgt cgtctgaaaa cccgtaatcc tccccctccg
     5341 gcggttgcgg ctgaggctct ggctgcgcgg ccttacgtcc acggcctttc gttttcgcgc
     5401 cacctttttt cgtcgcgtca gcactctgtg gctgcccatt ctgctgaggc ttcggttgcg
     5461 cctgagcgtt ctgctgtggt gcacgtccca gcatctgcac ggtgcccgtg gtcttaacaa
     5521 gaatttcagt gacgtaacgg gtgataccgt tatcttccca gctacgggtg cgaagctgac
     5581 cttcgatgta gacctgcgca cccttgcgca gatattcacc tgccacttcc gcgagcttgc
     5641 cgaacagcac cacgcggtgc cattccgtct gctcccgcat ctcccccgtc tgtttgtcac
     5701 gccagctttc tgatgtggcc acctgcaggt ttgccactgc gcccccgttg gggatgtaac
     5761 ggacttccgg atcattgccc agacgcccga cgaggatgac cttgttgata ccacgtgcac
     5821 tcataagaac ctcctgactg aaagatgtga gataaaccgg ataccggcga cgggacgttc
     5881 atgtgcccct tcagaggagc gaaagctcct cctgccgtcc acttcacagg ggtttctccc
     5941 cgcagtggac ctttccgatg ggggcccgtt taccgcctca cgggacagct gtccaggggc
     6001 ggacgcaaaa tttttagccg ggttcatggc taaaaatttt gtggccggtg catctcaccc
     6061 tggacagatg gaacgggagg gggttacgtc tgccccccgg aaagtccaca gcgggaaaca
     6121 ggccgcaggg gtggcggcag gagggaggac tgctgcgcca tcaggcgcag tttcctcaca
     6181 caccgtcagg tgtgcaggga gcgcaggtca cggcgcgaca ggggcggcgc gaccggaacg
     6241 gtcgctgcaa gccgggtttt cac
//
LOCUS       DAEBXN010000179         6254 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.180, whole genome shotgun sequence.
ACCESSION   DAEBXN010000179 DAEBXN010000000
VERSION     DAEBXN010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6254
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.180"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="KJL99_003995"
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="KJL99_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HBD4438542.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTNRSAEGIRL"
     gene            1568..2296
                     /gene="gadW"
                     /locus_tag="KJL99_003996"
     CDS             1568..2296
                     /gene="gadW"
                     /locus_tag="KJL99_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HBD4438543.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="KJL99_003997"
                     /pseudo
     CDS             complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="KJL99_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
     gene            complement(5797..6054)
                     /locus_tag="KJL99_003998"
                     /pseudo
     CDS             complement(5797..6054)
                     /locus_tag="KJL99_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
BASE COUNT         1659 a         1459 c         1476 g         1660 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attaccttta atgtcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacgtttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgacctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ttgaacgttt tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacgtgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aagggaattt ttacggcact aaccgttctg cggaaggaat aagattatag
     1201 agttttactc agacataaaa aaaacccggc acaggggacc gggaagagga tagtctgccg
     1261 tctccagact aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca
     1321 gtaagttaaa tataactttt acaggaaata agatcagcca tttttttata aacataagct
     1381 atacgctgtg cgaaaatata ttcgtgctgc atttacttat tatcaattaa ctgttatgca
     1441 aaactacttt gtggataaat tttggtccta ccaaatctgg cagtttttgc gctaagaaac
     1501 agtctgtcat catttcatta gtatactgaa attgaaataa tcgcagtatg aaatataagg
     1561 gataatcatg actcatgtct gctcggtgat cctcattcgt cgttcattcg atatttatca
     1621 tgaacagcaa aaaatatcgc tgcataacga gagtatcctg ctgctggaga aaaatttggc
     1681 agacgatttt gcgttttgtt caccggatac gcgacgactg gatatcgatg agctgacagt
     1741 ttgccattac ttacaaaata ttcgtcagct accacgcaat ttagggttac atagcaaaga
     1801 ccgtctgtta attaaccagt caccccccat gccgctggtg acggcgattt ttgatagctt
     1861 caatgaatcc ggggtaaatt caccgatact gagcaatatg ctctaccttt cctgtttatc
     1921 gatgttttct cataagaaag aactgatccc cttacttttc aatagcatca gcactgtttc
     1981 aggaaaagtt gaacgcctta ttagctttga tatcgccaaa cgttggtatc tgcgcgatat
     2041 cgcggaaaga atgtatacca gcgagagtct catcaaaaaa aagttgcagg atgaaaatac
     2101 ctgtttcagt aaaatattac tcgcttccag gatgtcgatg gccagacgat tactcgagtt
     2161 acgtcaaatt cctctgcata ctattgcgga aaaatgtggc tatagcagta cgtcgtactt
     2221 tataaacaca tttcgacaat attatggtgt aacgccacat cagtttgcgc aacattcgcc
     2281 aggtaccttt tcctgacata ttttgcattt gaatattggt caggatctca cacctgcttc
     2341 atgtgaaact cttccctgat gatttctgcc gggctaccgg ctagttctct ttcgcagttt
     2401 gccaggcggt ataggtattt ttaggaggaa aagactcggc atgtttggga ttattaagct
     2461 gacaattcat acgattacgg gaatgtgggt cagtattgtg ttgttcaaat tgatgacgaa
     2521 tggctggagt ggtttctact ttcagtgctg tgtactaagc ctggtattcc tgacggttag
     2581 ctggttgttg agtggtgaat ggttagcagg aaagagtaag gctgaacctt cacgttcaac
     2641 cttactctca tttacacgtt acgctttttt aaagcgggca aagagatgtt ccactacaac
     2701 gaaaaagacc ggaacgaagt aaattgccag cactgttgcg gcaaacatcc cgcccatcac
     2761 gccggtacct accgcgtttt gcgcgccaga accggcacca tgactgataa ccagcggcag
     2821 cacgccgaga ataaaggcca gagaggtcat caggattggg cgtaaacgca tccgcgccgc
     2881 ttcgatgatt gcctctatcg gcgttttccc ttctttctgc atcatctcaa cggcaaattc
     2941 gacaatcagg atggcgttct tggcggaaag cccgatggtg gtcagcaaac caacctggaa
     3001 gtagacgtca ttacttaagc cgcgcagatc ggtggccagt aatgcgccaa cgacgcctaa
     3061 cggaacaacc aacatcaccg agaacggaat tgaccagctc tcatagagtg cggcgagggc
     3121 gaggaacacc acgaccagtg aaatggcata cagtgcagga gcctggtttg aggataacgc
     3181 ttcctgatac gacagtccgg tccatgagta gccgacgcct gccggaagtt tagcgaccag
     3241 gtctgccata aatttcatag cgtcaccggt acttttcccg gccgccgctt cacctaaaat
     3301 ctccattgac gggatgccgt tgtagcgttc caatcgcggt gaaccatagg tccattcggt
     3361 agacgagtag gcagaaagcg gtgccatcgt gccagaggcg ttgcgtacat accattggtt
     3421 gatgttatcc ggcaacatac ggaacggcgt gcctgcctgg acatacactt ttttcacccg
     3481 cccctggttg aggaagtcgt tcacgtagct gctgccgaag gcggtggaaa ttgtctggtt
     3541 gatatcagac agcgccacac ccattgcctc agctttcgcg gcgttgacgt tcactttgaa
     3601 catcggcgta tcttccaggc cgttcgggcg taccccggtg acctggtcag gcgattgcgc
     3661 tgccagtgat aacagctcgt ttcgtgccta ggttagtttt tcgtgcccca ggttaccgtt
     3721 gtccagcagt tccatatcaa aacctgacgc ggtacccagt tcagccaccg cgggtaagtt
     3781 gaacgggaag acgacggctt tattgatact gcttaacgca atcattgccc gctgaatgat
     3841 cgcggtaacc gagttttcct caccgacacg ttcagaccac ggcttgagac tgataaacgc
     3901 caggccgttg ttttgcccct ggccgctgaa gccaaagccg ccaacggtaa acaccgactg
     3961 aacattatct ttctcttttg taagataata atccgtcacc tgttgcagta ctttcgtggt
     4021 gttaaccatg gtggcaccgg aaggtaactg cgcggtggtc ataaataccc cctgatcctc
     4081 ttctggtaag aaagaggtcg gtgtacgcag gaacagcacc gccatcccgg cgcaaatcag
     4141 caggtagacc accatgtagc gaccggtaca acgcaacagc gagcgggtgc tatcggtata
     4201 gtgctgagtt gatttttcaa acagcgtgtt gaagcgtgcg aacagggcgt taggtttgtg
     4261 accgccttcc ggcgtggctt tcagaatggt ggcgcacagg gcaggggtca ggctcattgc
     4321 cacaaatact gaaagccgca tggaggagat cagcgtgatg gagaactggc ggtagatctc
     4381 cccggttgca ccgctcataa aggccatcgg cataaacact gcagaaagaa caacggcaat
     4441 cccgaccagc gcacgttgga tctgccccat cgatttatgc gtcgcttcct tcggcggtag
     4501 cttatcttcc gcaatgacac gctcgacgtt ctccaccacc acgatggcgt catccaccag
     4561 taaccctatc gccagcacca tcccgaacat agtcaacgtg ttgatggtga aaccgaccgc
     4621 cgacaagatc gcaaacgtcc cgagaataac caccggtacg gcaattgtcg ggatgatggt
     4681 ggcacggaaa ttctgcaaaa acagataaat gaccaggaag acgaggatga tagcctcaac
     4741 cagtgttttg aaaacttcct gaatagaaat ttcgataaac ggcgtggtgt cgtaaggata
     4801 aaccgtcttc agacttgcgg ggaaataggc tgataagcgg ttcagctcct ctttgactgc
     4861 ccgcgaggta tccagggcgt ttgctccggc agccagtttg atggcgatcc cggcaacagg
     4921 tttgccgtta tagcgcgcca cggtggaata atcttccgcc ccaagttcga cgcgggcgac
     4981 atcacgcagc agcacttgcg aaccatcttg ctgaactttc aacaggattt tgccaaattc
     5041 ttccggcgtt tgcagacgcg tctgcacaat gatcgaggcg tttagttgct ggtctgccgc
     5101 ctgtggcatg ccacccagtt gaccaccaga aatctggttg ttttgcacct taatctggga
     5161 aataacatcg gaaggtacca ggttatattt attgagtttt tgcgggtcca gccagatacg
     5221 catggcgtac tcggaaccaa agagctgtac gctaccaacg cccgcggtac ggcttagcgg
     5281 gtctttgata ttagacgcta catagtccgc gatatcgtac tggttgaggc tgccgttatc
     5341 agaaataaac gccgctacca tcaggatatt actgctcgac ttatcgacgc taatcccctg
     5401 ttgctgcacc gcttcaggta atgaaggcat agcgagttgc agtttatttt gcacctgaac
     5461 ctgtgcgata tcaggagatg ccccagtctc gaaggtcaga gtgatagagg cattacccgc
     5521 cgcatcactg gttgaagaca tgtacatcag gccatcaagc ccattcatat tttgctcaat
     5581 cacctgagtg accgagtctt ctaccgtttg cgcatcggca cccggatagg tagcgctgac
     5641 ggtaatggtc ggtggcgcaa tctgcggata ctgcgcaacc ggtaagttca tgatcgccag
     5701 accacctgca agcatcataa taatggcaag tacccaggca aaaaccgggc gatcaataaa
     5761 atagttagcc atgaaagtcc ccttaagcct gcaacgttat tgtttcgatt cggtgctggc
     5821 gttttcctgg ctggaggaaa ttgctcgtgc tttgataccc ggacgaatgc gttgcaaacc
     5881 ggaaacgatc acccgatcgc cagcctgcaa gccagaggtg acaacccact ggtcgccgat
     5941 ggctttgctg gcttcaattt cgcgtagctg cacgacatcg tctttatcca gaatgagcgc
     6001 cgttgcttta ccctgggcgt tgtgggtgac gccttcctgc ggcaccagta atataaagcg
     6061 cgcaaaatta ttcaagatat ttttgaaaat cattcaagat aggctatcgc gtgtgatagc
     6121 aaaaacattc aagataaaaa tgcttgatta ttaagcaatc aggaatggga gtattaagtt
     6181 tcgtgtttcc agtaaaacca gagagtattg aaatagctat cacctaactt tatcaggcga
     6241 tagctggtag atga
//
LOCUS       DAEBXN010000180         6155 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.181, whole genome shotgun sequence.
ACCESSION   DAEBXN010000180 DAEBXN010000000
VERSION     DAEBXN010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6155
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.181"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(88..1478)
                     /gene="tcyP"
                     /locus_tag="KJL99_003999"
                     /pseudo
     CDS             complement(88..1478)
                     /gene="tcyP"
                     /locus_tag="KJL99_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
     gene            complement(1611..2201)
                     /locus_tag="KJL99_004000"
     CDS             complement(1611..2201)
                     /locus_tag="KJL99_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBD4438544.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(2364..3032)
                     /gene="hxpB"
                     /locus_tag="KJL99_004001"
     CDS             complement(2364..3032)
                     /gene="hxpB"
                     /locus_tag="KJL99_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HBD4438545.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3179..3715
                     /locus_tag="KJL99_004002"
     CDS             3179..3715
                     /locus_tag="KJL99_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HBD4438546.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFAHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(3756..4616)
                     /locus_tag="KJL99_004003"
     CDS             complement(3756..4616)
                     /locus_tag="KJL99_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HBD4438547.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACDWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4722..5012)
                     /gene="ghoS"
                     /locus_tag="KJL99_004004"
     CDS             complement(4722..5012)
                     /gene="ghoS"
                     /locus_tag="KJL99_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HBD4438548.1"
                     /translation="MASGDLVHYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(5113..6042)
                     /gene="pfkB"
                     /locus_tag="KJL99_004005"
     CDS             complement(5113..6042)
                     /gene="pfkB"
                     /locus_tag="KJL99_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HBD4438549.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
BASE COUNT         1588 a         1546 c         1517 g         1504 t
ORIGIN      
        1 tatgagtcag tatgcatcgc cgcgatggaa catttaaaaa gtaaagcaac ggcccctata
       61 caaaacggac cgttgccagc ataagaatta acggtgtgcc agttcggcgt cgtcttcgct
      121 atccagaatg gctttatcgg tttgcttcag ccactggctg gtcagcgtgc cagctgtcat
      181 cgagccacta acgtttagcg ccgtacggcc catgtcgata agcggttcaa cggagattaa
      241 cagcgccacc agggttactg gcaggcccat cgcaggcagc acaatcagtg cggcgaaagt
      301 tgcaccacca ccgacaccgg caacgcctgc ggaactaacg gtaacaatac cgaccagcgt
      361 cgcaatccac atcgggtcca gcgggttaat gccaaccgta ggcgcaacca tcaccgcagc
      421 attgccggat acaaaccggc gcagccgttc tgaccaatgg ttgcaccgaa agaggcggcg
      481 aaactggcga tggattcagg aacgcccaga cgacgcgttt gtgcttccac attcagtggg
      541 atagacgcag cactggaacg gctggtaaag gcaaacgtca gcacaggcca taccttacgg
      601 aagtacttca gcggactcac gccattaatg cccagcagaa tgccatgcac agcaaacata
      661 atcagcagac cgaggtagga cgcgacaacg aaacttccca gtttgatgat gtcttgcaag
      721 ttagaacctg caaccacttt ggtcattaga gccagaacgc cgtaaggggt caactgcatg
      781 acaaggcgaa ccagtttcat cacccagctt tgtagggtat cgatagcggt taagacgcgt
      841 tcccctttcg gcgcatcatc cttcagcagt ttcaatgcag ctacgccgag gaatgcggca
      901 aaaattacca cgctgataat tgacgtcgga ttggctccgg taagatcggc aaacgggttt
      961 ttcgggataa aggacaagac cagctgcgga acgctctgat cagagacttt accaacatag
     1021 ttactttcaa tggcgttcag acgtgcagtt tctgcaccac cctgaaccag accttcagcc
     1081 gtcaaaccaa acaggttggt gaccagcaca ccgaccagcg ccgcaatcag cgtggtaaac
     1141 aaaagcgtac cgatggtcag aaaactgatt ttgcctaact gagatgcgtt atgcagacgg
     1201 gcaaccgcgc tcagaataga ggcgaagact aacggcataa cgatcatttg cagcagttga
     1261 acatagccgt taccaacgat gttaaaccac tgtacagaat ctttaagtac ctggctgtca
     1321 gaaccataaa tggtatgcag ggcaaggcca aaaaccacac ccatcaccag acccaccagc
     1381 acttttttcg ccagactcca ctgtttatgg cgggtctgag ccagcgcaaa cagcagtaca
     1441 acgaacacca cgatgttcgc aattaatgga aagttcatcc ccgttctcct gatcttatta
     1501 tttctatcac tgcgtagcag tcatagtgtt ggcgcaagat taacagaact gtgagctggc
     1561 tcttatattc aaatggaatt gcttattcca gaaaggtgat ttttgccttt ttagtattca
     1621 acctgatgct taataagccg atgaaactgg atctgcaagg tattgatcat ttgcgatgac
     1681 caacgaacag cgctgttctc gggtaatgaa tggggcatcc ataccgacca gacaaataat
     1741 gccatgcaga taaaacgttc cagttggttg cccttttggg gaaccaggat aggcaagcgg
     1801 aaacgccagc ggcatggcca gagcagggga acaccggcgg gtgtcagcat atcggcaagt
     1861 atgtggctca aataacccag caccattcct tgtagcgcat cagccggaat gaaccagcct
     1921 tccggaacct taaggtaaaa ggttgccagc agcgcaaata ccgccagcag actgtgggta
     1981 aaaccacgat gcccaaaagc gcgggcgatc ggttttgata tccattttaa tcgctgccca
     2041 agaaacgact ttgggtgatc gatgtccggt aacaaacacg tcaggattgc ggaagggaca
     2101 atatgccacc agtcaccctg tgccagcacg ggcgtcagct cggcattttt ggcaaatacc
     2161 gcacaagcaa tagaaaagag aaggtgacct tccgccgtca tgataaaact cactaaactg
     2221 tcgatacgta cagtatagga tttttataca gtgcacggaa gtggtgacgg tgtaactctt
     2281 tctcttaaat tgacgattaa tccgtcagtt gaatgcaaat tcagataaag aaccaggggc
     2341 agcgtgatca ctgcccctgg tcattaaccg agaaggtctt ttgcggtgag ttctgtcagc
     2401 gatgagagtt tgacgtttgc taatacaaaa cgtggatcat tttgcgcttc tggcgcagga
     2461 acgacgatgg aacgcatgcg ggctgcttta gaagcgatca tgccatttac cgagtcttcc
     2521 agcgccacgc aggtcagtgg gtcaacgccc agttttgctg cgcagtcgag atatacttgc
     2581 ggatgcggct tgctgtaagg cagtttttca gccgaggcga gggcatcgaa actgtcgcgt
     2641 aagtcaaaca tggtcaacac tttttccagc atatgtagtg gtgacgcgga ggccagtccc
     2701 accaataaac cttgttcttt gcataacgca acagcttcgc gcacgcctgg taataatgga
     2761 cgtgtctctt caaccagtga aatggcacgg gcaataaccc gttctactac ttcctgacgg
     2821 cttggcccat tccacggttg ccgggcgtac caaagatcga ccaccatatc gatgcgtaaa
     2881 cctaaggtgt ccggcagctc gttacgacgg gagatatcta cccccaggct tgccatcaca
     2941 tccagttcgg ctcgatccca taaaggttct gagtcgataa gtaatccatc catatcaaaa
     3001 attgcagcaa gaatctgacg cggggttgac attacaacct ctcctttaga gcattatgtg
     3061 taaggtatta agccgtgctt atgattaagc gcctatcata tcgcctttaa agaatgggcg
     3121 aaaacagata tctacaggta aactaagtga aacgacgcgt cttaatcaag gagaactcat
     3181 gacgtatcaa caagctggac gcattgctgt tttgaaaagg attttgggtt gggtgatttt
     3241 tatacctgca ttgatctcta cattgatttc tttgctgaaa tttatgaata cccggcagga
     3301 aaaccaggaa ggcattaatg cggtcatgct cgactttgct catgtcatga tcgatatgat
     3361 gcaggcgaat acgccttttt tgaatctgtt ctggtataac tccccgaccc ctaattttaa
     3421 tggtggtgtg aacgttatgt tctgggtgat tttcatcctg atttttgtcg gactggcgtt
     3481 gcaggattcc ggtgcccgga tgagtcgcca ggcgcgcttc ttgcgggaag gtgttgaaga
     3541 tcaactcatt ctggaaaaag ccaaagggga agaggggcta acgcgtgaac aaattgagtc
     3601 ccgtattgtt gttccgcacc atactatttt cctgcagttt ttctcactat atatcctgcc
     3661 ggttatttgt attgctgcag gttatgtgtt cttttctctg cttgggttta tttaatcaaa
     3721 aggccgcacg gagcggccat tatcctcaac ccatatcatg ctgctaataa tctatccaat
     3781 gactgctgag caataaccaa atgctgaccg ccaaataatc ttgcacgatt taacagcgtg
     3841 tagagttggt aaaccggttg acgttcaagg aaatcggcag gtagcgggga tactgactga
     3901 tagccgtcat agatttgtgg cggctgttca gtatgcagcg gtaacatcgc taggtcgcac
     3961 tctcggtcac cccagtcgca ggcagggtcg aaaatgtacg ggccatccgg acccagcgca
     4021 cagttgccgg accataaatc gccgtgcaac agagaaggct gcggttgatg cgaggccaga
     4081 cgctgctgaa tatgctcgac gatggcgtcg atattgccga aagcgatccc tttctctgct
     4141 gccagttcca actgccagcc aatccgttgt tcagcaaaaa acgttgacca gcgacgttgc
     4201 caggtgttgg gctgtggagt tgtggagagc gagttatcaa aatcgaggcc aaattgtggt
     4261 tggtcactcc attgatgtaa acgcgcaatt tgctgaccaa gaataaatgc gctatgcgca
     4321 tccagcggac gaggtgggag ataatccatc accagaaaac tgtagtcacg gtcagcgcct
     4381 actgcccaaa ccttaggcac ggtgacggtt ttactacgcg acagtaactc cagttggtcg
     4441 gcttcggcgg taaaaccggg aagcagttcc ctttcatcac atttgacgaa aaagtcatgt
     4501 cctgcatagc gcaaatgcca tgcggcatgg acttctccgc caggcagttc attacgcagt
     4561 tcgatttcgc cttcacctaa ctgctcgctc aaaagacgac tgattgcctg ccacatgatg
     4621 tctctcccat gttgtctgcc agatcataag gttagcgcaa gaatgcggtg taaaaacacg
     4681 agctaacgca caccacagac tgattattgc ccaaaaacca tttattttct gttgctattc
     4741 cattcctccc aggtcgtgat ggtgatttca ggatctttgc cggttgcact ttgggtaagc
     4801 cccgaaacca gttctctaat ttcttcttca ctttgggtac tgataagtcc aaaagtgtta
     4861 gtccccagct catggatatt tccctcatca tccgtcatgg tgagcaaaaa accgtcgcga
     4921 gtcaggtaat tattcaactc gttaatttca gtcaatgtat cctcatgcaa catgacggtt
     4981 atgacataat ggacaagatc gccactggcc atagttcacc cctttgttat cacatgactt
     5041 ttgagcatag tcggagaaac gcgttgccga caggttggtg gtgattcccc caatgctggg
     5101 ggaatgtttt tgttagcggg aaaggtaagc gtaaattttt tgcgtatcgt catgggagca
     5161 cagacgtgtt ccctgattga gtgtggctgc actccccgca gctacgccaa aacggaccat
     5221 ctcttcaaga gaggcatttt ctgccagttt cagtgtcatc gcgccgacca tgctgtcacc
     5281 agcgccaacg gtactctggc ttttcaccgg tggtggcacc acctgaatgc agttttcact
     5341 atcgacaccc agcgctcctt gtggaccaag ggaaacgaca acccgtttgg ccttgccgct
     5401 attaacgatt tcctgcgcgg ctttgcggac atcgtctggc tgggtgagtt cgcgattcac
     5461 cagcgcactg agttcttttt ggttaggctt aaccaactcg atgttaccaa tcgccagtgc
     5521 tgcacttaac gcttcgccag aactgtcgat gatgcagcgg atcccttggt tttgcgcagc
     5581 ggaaatcagt tgggttaatt tttccagctt cacacctggc ggcaggcttc cgcttatgac
     5641 caggatggcc ccggattcaa tttccagaac ttgctcttca agctggcgaa actcatcttc
     5701 atttaatgcc gcgcctggca taacaaaacg atactgttca ccgcttgctt ccacatgtac
     5761 gtgtaaattc tgccgggtcc agtctttggc ttctacagta gcgacgggga cattttcatc
     5821 cgccaacagt gaaaccaggt gttcgccggt cgcgccaccc gccgggaaga tcgctgtggc
     5881 actgcctcca agatgggcaa tggcgcgggc gacgttgatg ccgccgcccc cgggttcgaa
     5941 caccggtgcg gtacagcgca gttttccttc gggataaatt tgcggggtaa ttgttgcgct
     6001 atcgagagag ggcgcaagtg tcaacgtata gatacgtacc atcatttcct cctataggct
     6061 gatttcagtc tggcaccgaa tcagcggaaa gtgaagaaat taacaatatg atttattaga
     6121 gctttattta aaattttgca gataaatata tataa
//
LOCUS       DAEBXN010000181         6058 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.182, whole genome shotgun sequence.
ACCESSION   DAEBXN010000181 DAEBXN010000000
VERSION     DAEBXN010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6058
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.182"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(<1..143)
                     /locus_tag="KJL99_004006"
     CDS             complement(<1..143)
                     /locus_tag="KJL99_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HBD4438550.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQ"
     gene            379..651
                     /locus_tag="KJL99_004007"
     CDS             379..651
                     /locus_tag="KJL99_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HBD4438551.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(617..1024)
                     /gene="nudG"
                     /locus_tag="KJL99_004008"
     CDS             complement(617..1024)
                     /gene="nudG"
                     /locus_tag="KJL99_004008"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HBD4438552.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELDIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            1111..1731
                     /locus_tag="KJL99_004009"
     CDS             1111..1731
                     /locus_tag="KJL99_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBD4438553.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(1740..3047)
                     /gene="ynjE"
                     /locus_tag="KJL99_004010"
                     /pseudo
     CDS             complement(1740..3047)
                     /gene="ynjE"
                     /locus_tag="KJL99_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
     gene            complement(3114..3767)
                     /locus_tag="KJL99_004011"
     CDS             complement(3114..3767)
                     /locus_tag="KJL99_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBD4438554.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(3767..5238)
                     /locus_tag="KJL99_004012"
                     /pseudo
     CDS             complement(3767..5238)
                     /locus_tag="KJL99_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
     gene            complement(5211..6058)
                     /locus_tag="KJL99_004013"
                     /pseudo
     CDS             complement(5211..6058)
                     /locus_tag="KJL99_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         1427 a         1621 c         1599 g         1411 t
ORIGIN      
        1 atctggcgat attttggatt ttgttcaaga aaaggccaga gtgtggtcat tacttcacga
       61 acggcttgcg cgaactcggt ttgattcggg tcgcgctttt ggacatggtt gaggaatgac
      121 tccagagaat atgtctgatc catagatata aaaaccttat atattaatac gattgtgctt
      181 atgttgttta tgtcatgtgc ttttgcagtt ttcgactata ccatcaagaa tgtgacggta
      241 agcaagaaaa aaatgaccca ggaaagcaaa ttaataacga gagtaatctc ataaccaaat
      301 gcgataactc agacgctaaa gttttgccac ccggctaccg ttatcagtag cagagacaat
      361 aacgttaagg agttccctgt gagtcgagca ttgttcgccg tagtattagc atttccatta
      421 attgcactgg ctaacccgca ttatcgtccg gacgtggagg taaacgtacc gccagaagtg
      481 ttcagttcag gcgggcagtc agcgcagccc tgtactcagt gctgcgttta tcaggatcaa
      541 aattattctg aaggggcggt aattaaagcc gaaggaatac tgctgcaatg ccagcgtgat
      601 gacaaaaccc ttagcactaa tccgctgctc tggcggcgcg taaagccata aacgcctcta
      661 ataatggaat gtcagcaggg gccagcggat attgcagcgc ctcttcaggt gagcaccaga
      721 ccagcgcctg atgttcatgt gcctgtaacg tcccgtggaa gtcgggtacg tgccaggcat
      781 gaagatggat aatccggccc gaaacttctc gctgatggct ggcaacatac tcacccacag
      841 ttgcttcgat gtccaattct tcgtttaact cacgcaccag cgcctgctgc tggctttcat
      901 ccagctcgac tttacctccg gcaaactccc ataatcccgc ctgatcgctc tgggcggggc
      961 gttgcgcgag taaaattttg ccatcacgtt caatgatggc ggcaacaact tcaatcattt
     1021 tcataaatta cacacgggca gataagcgaa aaatgccata atatccgcac tgttgctcaa
     1081 tcagaaaggc tttatattta aggagtgcct gtgctagacc gccatcttca tccccggatt
     1141 aaaccgttgt tgcatcagtg cgtgcgggtt cttgataaac cgggcattac gccagatggt
     1201 ttaacattag tgggatttgc catcggcgtg ctggctctgc cgtttctggc gctgggctgg
     1261 tatctggcgg cgctagtcgt cattttgttg aacaggctgc ttgatggtct ggacggtgcg
     1321 ctggcgcggc gcagagggct taccgacgcg ggcggctttc ttgatatttc tctcgatttt
     1381 ctcttttacg cgctggtgcc gtttggcttt attcttgctg caccggagca aaatgcgctg
     1441 gcaggcggct ggttgttgtt tgcgtttatc ggcacgggca gcagttttct cgcctttgct
     1501 gcactggcag caaaacatca gattgataac cccggttatg cacataagtc gttttactat
     1561 ctggggggat taaccgaagg cacagaaacg atcttactgt ttgtgctggg atgtttattc
     1621 cctgcatggt ttgcgtggtt tgcgtggatc ttcggtgcgt tgtgctggat gacgaccttt
     1681 acgcgggtgt ggagtgggta tctgacgctg aagtcgctcc agcgtcagta attgcaatgt
     1741 tatttgctac tgcccgggcc gcgttcaccg gttgcaaccg gattttttgg atcgctgctc
     1801 cattcgtacc agccgccgtc atacacggaa acattcttcc aacccatggc gcgtgcgtac
     1861 ataaaggttt cggacgcgcg ccagccggtg ccgcagtaga atgaaacttg ctgatctggt
     1921 ttgatattcc atgctttcca catagcggta atatcatcgg cgctacgcat ggtgccatcc
     1981 gggttataga aatcttccat atgcgtcgag tcgctaccag cgtgtcccca acgtgctccg
     2041 gctatttcac cttttggttt aatatagctg taaccgctgg tcgtaccgat aaattctggc
     2101 cacgaacgaa tgctcaccag cgatgcatcc tggcgatgca gcagtccacg ggcctgttcc
     2161 atatcgagca tcaactgcgg ttgtgccggg atcttcacgc cgaaatccgg ttccgctttc
     2221 actttcggtg gcgttcctcg ctcaacaggc agtcccgcgt cggaccaggt ttgccagccg
     2281 ccatccagca gacgcacatc tttcacgcct gcgtagagca taatttgcgc cacacgcgct
     2341 ccagcgtata cgtcacgccc gtacagaatg accgtggtgt catggcgaat gccgtgtttt
     2401 gccagcatcg cttttagttg ttcatcagaa actttgttcc acagcggttc gctttccact
     2461 tcgttggtat cgatgtagtc agcgccagga atatggctga taaggtaaag cttaggagcg
     2521 ccctaggccg cttcaatgac tttccagtca ccgacaggtt tcgccgtaac ctctttacct
     2581 tgttgcaggt catgcagcca ttgcggataa accagctgct caaagtgcgg cagtttttgt
     2641 agacgggaag gttcgcttag cgcgtcactc agaatggaga tatgcgtaaa acctgctttt
     2701 tgcagtcgcg ttttgacggc gtcaacatct ttgccattac cgtacagcgc caccggggca
     2761 tcggctttca ggttatgttg cttgatccac gcgttgagct gttcggtgct cattttgtcc
     2821 agccagctag cagagaggtt taaggcggca ggttcatgac cagaagggcc atttaaggtt
     2881 tgtggccagc cgttataaaa cgcgctgggg cgagtatcta tcgctttgcc attttgttgc
     2941 tgaagttggt caagtgtaag aggcttcgcc agttcagcgg cccacgaaga agcgcaagcc
     3001 agccctaaag ccattgccag cgcggtcatt tgagaaacac gtttcatcga aaatcccgac
     3061 attgtagttt tgagtgcgcc attctgaact gacgcattga gaaaaacttt gcgttatcgc
     3121 agtttgttgt aattttctga ccactgcgcc atatccagaa cagaactatc agcaggaaca
     3181 tcctggagat cgtgcgttac ctgaacgacg gggatcgcca gggcgcgaac ttcgctgaac
     3241 acccactggc gaaaattatc gcgcagagcc acatcaagac ggctgaatgg ctcatccagg
     3301 agtaacgctt ttggctgggc gagaagggcg cgtagtagag caacgcgcgc tcgctgaccg
     3361 ccagacaaag tggcaggatc ctgatggaaa gctccgtcaa ggcctgaacg ctcaagtgca
     3421 tcattcacgg cattacgtcg ggcattcccc ttaagtgtcg ccggtagcgc cagcagtaaa
     3481 ttttgcccga cgctgaactg gtcaaataac agtgcatcct gaaaaagaat gccaatctga
     3541 cgctgtgcgg tgggtagcat gtcaatccgt tgctcattga gccatagctc acctgtacaa
     3601 gaaaactgtc cggccagtgc accaatcatc catgaaaaca gagtggattt tccacagcca
     3661 gacggcccca ttaacgtgac gatgtcacct ttatcaaccg taaagttaac gtttgtcagc
     3721 aagcggcttt ctggtaaacg tagcgaaaca tttttcacgc agagcattag cggattcctt
     3781 gtctgacata acctacccat tttgcgacta acgcggtcag ggcaaaaata ataagcggta
     3841 acaacagttg ccataacgcc tgggcggcga gaataccgtt gctgccgccg ctgcttaatg
     3901 ccaccgcctc agtggtgagc gtcggaaaac gccccgcgcc cagccacagc gtcggcatat
     3961 actgcgcaat actgactgaa aatcccaccg cgaaggcaat cagcacaggg cgcaacatga
     4021 gtgggcattt cacgtaaaag aagattttgg cccgcgacca gcccagtgtt tgcgcaatca
     4081 aaattaaccg tgaatcaatg cgctgccagg cgggttgcag gataaacagc atccacggca
     4141 ttacccacag cagatgcccc cagaccaccg ccgtccagct tccatccagg ttcagccata
     4201 gcgccagcgt gtactggccc gccaccagtg gcagagcggg caatagaatg ggcaaccata
     4261 gccacaactg gcgacgctgt gccccccatt ccagccacag cagtaacagg agcaaagcga
     4321 tgaatgttgc taccagcccc attgtcagac tgttgatgag tgcttcacta ttgatcgtcg
     4381 actgatccgc gaggatcgcc agcagaacca cacacagcac accggttaag ggtaaaaaac
     4441 tcgccagcgt agtgcctggt aataaaggtg tggcaggctt gcgaatgcca tctacgcggg
     4501 gaatagtgcg ccgccagctg cgccaaaacg taggcggcga gtaacagcat cagcagcaga
     4561 ctggcgagtg cgcctttcgt ttgttgatcc gcgtcgccct gagttaacca ctgccagcta
     4621 attactgcca gcgtcggtgg attacctggc ccaagaataa tcgccacatc cacgaccgaa
     4681 agcgaccagg caacaatcgc cagcatcgcc attgccagcg gctgtagccc agcgaatcca
     4741 gcacaatgac ctgctgcaac agccgttttt cgctcaatac tgccactaag atccacagca
     4801 gaaatgcgct ttctttcact gcaagggtga ggcccagacc gatccaaatc ggtccattgg
     4861 cggagtgaaa tacgggaaat agtcatatag cagccctccg tcagcaaaga gcagcagggc
     4921 gctggtggca aaagccacat ggggaatggc gagcagccac ggcagacggg cgcacatacg
     4981 ctgccatttc ggcccaggcc atagcgccac aatcaccagc agggcaatca acaatgcccc
     5041 gaccgccgcg atggttgtcg acaccagcgt tgccagtaat gcctgcggta actgtggatc
     5101 ggcaaataac gcctgccagc gtgtcaacga caacgcaggc gatatcagcg tgagagctgc
     5161 cgggatcagc ggtgcataaa tcaccgccac catcgcccac agcaaaaaga ttaatgcgta
     5221 ccgtaacggc gtaaccattc ttgttccagt gcattcaccc atcctgcgtg cggttcagcc
     5281 agtaccggcg gcagatcctg cggcattctt gattgcaata tttcgcgctg cccgtcaggc
     5341 agtttttgcg gatcgagaac agaaggatcg ccccagacaa cgggatctgc tttacgcagt
     5401 tgcgcatcgg gtgagagcag gaaattggca actaccttcg ccgcagcact ggcattcgcg
     5461 ttggctggaa tggtgacgaa atgcacgttg ccaatcatcc cctcgcgaaa gccaaaactg
     5521 taactgcttg ccggcaaatc gccgctggcg attttttgct gcgcatgtgc ggggttaaag
     5581 gtcagcgaca ggcgcaatgt gccggctttc agcagagcat ccatccgcgc gggcgaaggc
     5641 gggaaatcct ttccttcgcg ccacaaatac ggatgcagtg catcaagata ttgctacaag
     5701 ggcgcagtga cacgggcgaa agtcgcatcg tccggcgctt cttttaatgc ggcgggatct
     5761 ggcgtcagca taatcagcaa ctgttcaaga aacgccgtgc cggtaaagtc cggtgggcgc
     5821 ggataagtaa ccgtgccggg attggcttta gcaaactcca gtaaggcttg cggcgtttgt
     5881 ggtggctgtg gcgtaacatc gcggcgggcg ataaacgtca gttgtgcgcc gccccagggc
     5941 gattccgcac cttgtgtcgg cactgaaaaa tctccccgca ccggcagctg tgtgtcgaca
     6001 tagcgccagt tgggcagagt ctccgcccag cccgtttgta ataaattggc ctctttta
//
LOCUS       DAEBXN010000182         6035 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.183, whole genome shotgun sequence.
ACCESSION   DAEBXN010000182 DAEBXN010000000
VERSION     DAEBXN010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6035
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.183"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            413..1321
                     /locus_tag="KJL99_004014"
     CDS             413..1321
                     /locus_tag="KJL99_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HBD4438555.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENIFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIENRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMLPGSTVPFISTK"
     gene            complement(1367..3358)
                     /gene="tkt"
                     /locus_tag="KJL99_004015"
     CDS             complement(1367..3358)
                     /gene="tkt"
                     /locus_tag="KJL99_004015"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBD4438556.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            3636..4394
                     /gene="loiP"
                     /locus_tag="KJL99_004016"
                     /gene_synonym="yggG"
     CDS             3636..4394
                     /gene="loiP"
                     /locus_tag="KJL99_004016"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HBD4438557.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLHQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRISADGIK"
     gene            complement(4600..5520)
                     /gene="speB"
                     /locus_tag="KJL99_004017"
     CDS             complement(4600..5520)
                     /gene="speB"
                     /locus_tag="KJL99_004017"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HBD4438558.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5656..>6035)
                     /locus_tag="KJL99_004018"
     CDS             complement(5656..>6035)
                     /locus_tag="KJL99_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438559.1"
                     /translation="GMNYQLYVRNYSRNTNYEIVATADGLDVLNGKQGSLNNNGYIVN
                     AGDSLVIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQNA
                     LPPCSGQAFPADNNGYAPPPCRK"
BASE COUNT         1484 a         1575 c         1557 g         1419 t
ORIGIN      
        1 attgatgatt ctggaaaata atcactaagc cgcataaatt atccttattt tatttttagg
       61 gtaccgtccg gcggtaaaaa taaaatactc gtgcggttta atgaaagcaa ttgggtacgc
      121 gagaagaaaa accccacaaa ccaaaataat aaaaccttaa ataacaacaa tatgaaattg
      181 atatttttaa agtcaacttt gtatttgtta tgttcatcac aaaatcaaca ttttgtttgt
      241 tattgtattt gtgatgcata tctctattta tttattgaat gttcgaaaac atacttttct
      301 aatgcaatgt tttttcgcta taccccttta tagattctga attctcacct gtcggctgtt
      361 ttctcctcat ctcatgtgac atattcccat actttactca aggaagattg tcatgaaagc
      421 acctgtttca ttaacctcat ttcgcccaca aaaatctctt gctatcgcca ttggcgtgct
      481 tgccgtggtt gtgctgccat ttctctcgta ttacaccgtt aatgaaggtg aacgcggcat
      541 tttgctgcgc tacggaaaaa tcgtaaaagt tgccgaaccg gggctgggtt ttaagatccc
      601 ttttatggaa agcgtggaga aaatttccac gcgtaatcag gctgtggtct atcagggatt
      661 acaggcctac agccgcgacc agcaaccggc acaaatgaca gtatcagtca gcttccacat
      721 taaaccttca gaagcaggcg cggtttatac cacctacaac actattgaag cattaaaaga
      781 tcgtctaatt gtccgtcagt tgccaactca gctggagaac attttcggcc aatataccgc
      841 catcagcgcc gtacaggatc gtacaaaatt ggttcaggat ctgcaaaatg ccatgcgcaa
      901 agccgttgtt gggccagtgg taattgacgg ggtccagatt gaaaatattg atttttctga
      961 cgcttatgaa aagtcgattg aaaaccgaat gaaagcagaa gtcgccattg ccacgcgtaa
     1021 gcaaaatctg gaaacagaga aaatccaggc gcaaatagcg gtaactcagg cccaggcaga
     1081 agcagacagt aaactggcgg cagccaaagc agaagcggaa actattcgcg taagaggcgc
     1141 ggcagaggct gaaactattc gtttaaaaag tgcggctgaa gcagaagcaa tccgcctgcg
     1201 cggtgaagct ctgcgggata atcccggact tgtagccctc accactgccg aacgttggga
     1261 cggtaaactg cccgatacca tgctccccgg cagcacagtt ccgtttatct ctactaagta
     1321 aaaaaagggc cgctttcgcg gtccttctca caaaacgtct tactgattac agcagcgctt
     1381 ttgctttcgc aacaacgtta tcaacagtga agccgaactc ttcaaacagc agctctgccg
     1441 gagcagattc accgaaggtg gtcatgccga cgatagcgcc gttcaggcca acatacttgt
     1501 accagtagtc agcaataccc gcttctaccg caacgcgtgc agtaaccgct ttcggcagta
     1561 cggattcacg gtaagccgca tcctgcttgt caaatgcgtc ggtagacggc atggacacca
     1621 cgcgcgcttt cacgccttcg gcagtcagtt tttcgtaggc agcaaccgcc agttcaactt
     1681 cagaaccggt ggcgatgaag atcagttccg gctgaccagc acagtctttc agcacataac
     1741 caccgcgcgc gatgtttgcc agctgctctt cagtacgttc ctgctgcgcc aggttctgac
     1801 gggagaggat aagcgcagtc gggccgtcct gacgctcaac gccgtatttc cacgcgaccg
     1861 cggattcaac ctggtcacac ggacgccatg tagacatgtt cggggtcacg cgcagagaag
     1921 cgacctgctc taccggctgg tgagtcgggc catcttcgcc cagaccgatg gagtcgtggg
     1981 tgtaaaccat cacctggcgc tgtttcatca gcgcagccat acgtacggcg ttacgtgcgt
     2041 attccacgaa catcaggaag gtggaggtgt acggcaggaa gccaccgtgc agggagatac
     2101 cgttagcaat cgcggtcata ccgaactcgc gaacaccgta gtggatgtag ttacctgcag
     2161 catcttcgtt gattgcttta gaaccagacc acagggtcag gttagacggt gccaggtcag
     2221 cagagccgcc gaggaattca ggcaacagcg ggccgaacgc ttcgatagca ttctgcgacg
     2281 ctttacggct ggcgattttc gccggattag cctgcagttt agcgataaac tctttcgctt
     2341 tggcgtcgaa gtcagacggc atttcgcctt tcatacggcg ggtaaattca gccgcttcct
     2401 gcggataagc tttcgcgtaa gccgcaaact tctcattcca tgcagattct ttcgcctggc
     2461 ctgcttcttt cgcatcccac tgagcataga tttcagacgg gatttcgaac ggtgcatatt
     2521 tccagcccag ctgttcgcgg gtcagggcaa tttcagcgtc gcccagcggc gcaccgtggg
     2581 agtcgtgagt accggctttg ttcggggaac cgaaaccgat gatggttttg cacatcagca
     2641 gggacggttt gtcagtcact gcgcgcgctt cttcaactgc gcgtttgatg gatgccgcgt
     2701 catgaccgtc gatgtcgcga ataacgtgcc agccgtaagc ttcgaaacgc attgctgtgt
     2761 cgtcggtgaa ccagccttca acgtgaccat cgatagaaat accgttgtca tcgtagaacg
     2821 caatcagttt acccagcttc agcgtacccg ccagagagca aacttcgtgg gagatgcctt
     2881 ccatcatgca gccgtcgccc atgaaggcgt aggtgtagtg gtcgacaatg tcgtggcccg
     2941 gacggttaaa ctgcgccgcc agcgtttttt ctgcaatcgc cataccgact gcgttggcaa
     3001 taccctgacc cagcggaccg gtggtggttt ccacaccagc ggtataacct acttccggat
     3061 ggcccggagt tttagagtgc agctgacgga agtttttcag ttcttccatc ggcagatcgt
     3121 aaccggtgag gtgcagcagg ctgtagatca gcatggagcc gtggccgttg gacagcacga
     3181 agcggtcacg gtcagcccag gacggattct gcgggttgtg tttcaggaaa tcacgccaca
     3241 ggacttcggc aatgtcagcc atacccatag gggcacccgg gtgaccggat ttggctttct
     3301 gtactgcgtc catgctcagc gcacgaatag cattggcaag ctctttacgt gaggacattt
     3361 tgactccaga tcggatgatg aagggcacgc ccttaacgac ttgacgacag cgcgttttgg
     3421 gctacgccgg aaaatttgcc aacaatttac cgcaagccgc gcgtcatgta catggaacat
     3481 ccttttgccg cttcagaaat ctctggatca tgctcgcatg ttgcgcaatc tgctcgcccg
     3541 tccgctgcgc ttttccttat actgagactg agcgtcgatt cacctgcaaa cggcgcattt
     3601 ttagaataat cctgaccttg tgcggaagag aaaacatgaa aattcgcgcc ttattggtag
     3661 caatgagcgt ggcaacggta ctgaccggtt gccagaatat ggactccaac ggactgctct
     3721 catcaggagc ggaagctttt caggcttaca gtttgagtga tgcgcaggtg aaaaccctga
     3781 gcgatcaggc atgtcaggag atggacagca aggcgacgat tgcgccagcc aatagcgaat
     3841 acgctaaacg tctgacaact attgccaatg cgctaggcaa caatatcaac ggtcagccgg
     3901 tgaattacaa agtgtatatg gcgaaggatg tgaacgcctt tgcaatggct aacggctgta
     3961 tccgcgtcta tagcgggctg atggatatga tgacagataa cgaagtcgaa gcggtgatcg
     4021 gtcacgagat ggggcacgtg gcgttaggcc atgtgaagaa agggatgcag gtggcgcttg
     4081 gtacaaatgc cgtgcgagta gctgcggcct ctgcgggcgg gattgtcggc agtttatctc
     4141 aatcacaact tggtgatctg ggcgagaaat tagtcaattc gcaattctcc cagcgccagg
     4201 aagcagaagc cgatgattat tcttacgatc ttctgcacca acgcggcatc agcccggcag
     4261 gtcttgccac cagctttgaa aaactggcga agctggaaga aggtcgtcaa agctcaatgt
     4321 ttgacgacca tcctgcatcc gccgaacgtg cccagcatat tcgcgatcgc atcagcgcgg
     4381 atgggattaa gtaaagccta gtggtgtcga acgtaggtca gataaggcgt tcacgccgca
     4441 tctgacatta atggcacatt tgttaccttg tgcgcattgc cggatgcgat gctggcgcat
     4501 cttatccggc ctacgggtgc cgaacgtagg tcggataagg cgttcacgcc gcatccgaca
     4561 ttaatggcac gttttacccg tgcgcatcgc atctggtgct tactcgccct ttttcgccgc
     4621 ctgaatatac agcatctcca gcgccagcgt cgccgcagcc agagcggtga tttccgactg
     4681 atcgtatgcc ggagccactt ccactacgtc catcccaacg atgttgagat ctttcaggcc
     4741 gcgtaccagt ttaatggcgc gatcggaggt caggccgcca atcactggcg taccggttcc
     4801 cggcgcgaaa gcaggatcca ggcagtcgat atcaaaagtc aggtaaaccg gcatatcacc
     4861 cacaatctgt ttcacctggg cgataacgtc atccacgctg cgatcgttca cctggcaggc
     4921 gtccagcacg gtaaagccgt tgtctttatc aaactcggta cgaataccaa tctgcacgga
     4981 atgattcggg tcgatcagac cttctttcgg cgcggtatag aacatagtgc cgtggtcaaa
     5041 ttcacaaccg ttcgcatagg tatcggtgtg ggcgtcaaag tgtaccagcg ccattttgcc
     5101 gaaatgcttc gcatgagcac gcagcagcgg cagcgtaaca aagtggtcac caccgaagga
     5161 gagcatacgc ttaccggcag ccagcagctt ctcggcgtgc gcctgcagct tttcgctcat
     5221 ctcacgggca tcgccaaagg catataccag atcgccgcag tccacgacgt tcagacgctc
     5281 acgcatgtcg aaattccacg ggaagcggtt gtgttcccag gccagattcg tcgaaacctg
     5341 acggatcgct gccggaccgt ggcgaccacc cgcacgacca gaagtggcca tatcgaacgg
     5401 cacgccagta atcacccagt ctgcatcgct gtcatacggc tggaagttca tcggcaggcg
     5461 taaaaaacca aaggcattgg aaaccagtga gttatcgtat tgatgaccta aggtgctcat
     5521 ggatggactc ctattacaaa gtcgatataa aaaaaccctt ccgcgtcgtt aggcccgacg
     5581 aggaagggtt ggatttgcga aacacaagtt tgtgctaatt atcgccttaa atgggcgagg
     5641 catcaagcga ggttgctatt tccgacacgg tggcggcgca tagccattgt tatctgccgg
     5701 aaaggcttgt ccagagcacg gcggcagcgc gttttgcgcc gggccttgta attcaaaggc
     5761 ggcaaagccg attaccccgg tattacgcac atcgccttgc gccgaattgg ctgcgtagga
     5821 atcagcaacg ttggcgaact gaaaagccgc ttcagtatgt ttatctttgc ggaatccttt
     5881 aatcactaac gaatctccgg cattcacaat atagccgttg ttattcagcg aaccttgctt
     5941 gccgttcagc acatccaggc catcagccgt agcaacgatc tcgtagttgg tgttacgact
     6001 gtaattacgg acatataatt gataattcat tccga
//
LOCUS       DAEBXN010000183         6014 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.184, whole genome shotgun sequence.
ACCESSION   DAEBXN010000183 DAEBXN010000000
VERSION     DAEBXN010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6014
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.184"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            63..581
                     /gene="mddA"
                     /locus_tag="KJL99_004019"
     CDS             63..581
                     /gene="mddA"
                     /locus_tag="KJL99_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HBD4438560.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGHWLDLTFMQL
                     QLDERTEPDAIG"
     gene            578..1027
                     /locus_tag="KJL99_004020"
     CDS             578..1027
                     /locus_tag="KJL99_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBD4438561.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMARITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(1028..1261)
                     /locus_tag="KJL99_004021"
     CDS             complement(1028..1261)
                     /locus_tag="KJL99_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HBD4438562.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(1347..1520)
                     /gene="ortT"
                     /locus_tag="KJL99_004022"
     CDS             complement(1347..1520)
                     /gene="ortT"
                     /locus_tag="KJL99_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HBD4438563.1"
                     /translation="MFLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            1715..1810
                     /gene="yncL"
                     /locus_tag="KJL99_004023"
     CDS             1715..1810
                     /gene="yncL"
                     /locus_tag="KJL99_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HBD4438564.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(2212..3021)
                     /locus_tag="KJL99_004024"
     CDS             complement(2212..3021)
                     /locus_tag="KJL99_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HBD4438565.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNKIFLNHKGKKSYK
                     ERNLFLFSKGDKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIA
                     SNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWI
                     GNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIK
                     WWNYNYSDLPDNNKCDDINYFVEEMNRLISNGNIQERDYKKFNLSEVFRGL"
     gene            complement(3231..4655)
                     /gene="patD"
                     /locus_tag="KJL99_004025"
     CDS             complement(3231..4655)
                     /gene="patD"
                     /locus_tag="KJL99_004025"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HBD4438566.1"
                     /translation="MQHKLLINGERVSGEGEKQPVYNPATGDVLLEIAEVSAEQVDAA
                     VRAADAAFAEWGQTTPKARAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(4677..5471)
                     /locus_tag="KJL99_004026"
     CDS             complement(4677..5471)
                     /locus_tag="KJL99_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBD4438567.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(5461..5808)
                     /locus_tag="KJL99_004027"
                     /pseudo
     CDS             complement(5461..5808)
                     /locus_tag="KJL99_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
BASE COUNT         1565 a         1529 c         1346 g         1574 t
ORIGIN      
        1 tgcttcgggc tgttttcgat agtattgata acatcatcat cattatttta ttgaggccag
       61 acatgtccat ccgttttgcc cgcaaagccg actgtgctgc cattgcggaa atttataacc
      121 acgccgtgtt gtatacggcg gctatctgga atgaccaaac ggtggatgca gataaccgca
      181 ttgcctggtt tgaagcgcgg accatagcag gttatccggt gctggtaagt gaagaagatg
      241 gcgtagttac gggatatgcc tcgtttggcg actggcgtag tttcgatggt tttcgccata
      301 ccgtggaaca ttcggtttat gtccatcccg atcatcaggg caaaggtctg gggcgtaaat
      361 tgttaagtcg attgattgat gaagcgcggg attgcgggaa gcatgtcatg gtcgccggga
      421 tcgaatcgca aaatcaggct tcgctgcatc tgcaccagtc gctgggattt gtcgtcaccg
      481 cacaaatgcc gcaggtaggc actaaatttg gtcattggct ggatctgaca tttatgcagt
      541 tgcaactcga cgagcgcact gaaccggacg cgattggatg aatcagtcgc tcacccttgc
      601 ttttttgatt gccgccggaa ttggtctggt agtccagaac acgctgatgg cgcgcatcac
      661 ccagacctcc tctaccattc tcatcgccat gttgctgaac tcactggtgg ggattgtgct
      721 ctttgtttct attttatggt ttaaacaagg catggcgggg tttggcgaac tggtgtccag
      781 cgtgcgctgg tggacactta tacctggctt gctgggttcg ttttttgtct tcgccagtat
      841 cagcggatat cagaatgtgg gagcggcgac aactattgcg gtactggtcg ccagccagct
      901 gattggcggt ctggtgctgg atatcttccg cagtcatggc gtaccactac gggcgttgtt
      961 cggcccaata tgtggcgcaa ttttacttgt ggttggcgcc tggttggtgg ccagacgctc
     1021 attttaatca gaggatggtg ccgcctttgg ttaactgttc gtggcgcgct tccatatctt
     1081 ctttatgctt gcgaccgtga tgagcaatag cggtgcgcaa acgctgctgc tgggtgtagc
     1141 gatcttcccg acttaactcc gcgtcatcgc tcagggcgat taataattcg ttcatatggg
     1201 caatgacgct ctcttcaatc gcggcatcca cgcgcgcgat gacttcatcc agatgcgaca
     1261 tactttctcc ttagccactt gtgaaattta tcgattaatc caaatgatat aagtctgcgt
     1321 ggtgccgtca agccagtgag taagatttaa aacagtgaaa acaacagcgc caccgggaaa
     1381 ctcatcggcc atgtggcccc caccagaaag gcgcttaaga aacggatgcg tttcttatcg
     1441 tgagaaagaa accaggtgat aagaaatgcg attgctgcca taaccgcata aaaaacaagc
     1501 atatgttgat agagaaacat gtacgggcgt ccagccatat gaataaaacg cgcgcaaata
     1561 tgcgccattt tttgacgtgc atcaagtttt ttcaccagtt tgagaaaaaa tcatcaaaca
     1621 gcaacttgcc cttatccatc ttcagaatga gtattattgc ggattttctt aacccgtact
     1681 atacacatag gggcaatgat aaaaggtggc aaaaatgaat gtttccagta gaactgtagt
     1741 actgataaat gtctttgctg ctgttggttt gtttactctt atctctatga gatttggctg
     1801 gtttatttga tgtagtgatt ttgcacgcag cacgtactgt cagagaaatc aactctctga
     1861 cagtacgcaa gatcttctca tactctccaa gtttgcaaca ttatccttaa tatttacttc
     1921 tttttgctgg catctacatt gaaatgagta gaaaacatcc ccctactgtc gtctatacaa
     1981 acagcttact gattcaaacg gaatcttgtt ttcacatttt tctatcacga atactttata
     2041 cgcgcgggag tctataaatg ctttatactt tcaaggaata acgtaaatat aaaaagatgg
     2101 cgtaaagtat atgcatggca ggatgtttgc aacctccttg atgacgctgc cccattgaaa
     2161 ttgcaaaccc cgccctggtt atcttgccca tttatcatgc aaaacagata attataggcc
     2221 tctgaatacc tcagaaagat tgaatttttt atagtccctc tcttgaatat ttccatttga
     2281 tatcaatcga ttcatctctt caacaaaata atttatatca tcacacttat tattatccgg
     2341 aagatctgag tagttataat tccaccattt tattctcaac agttcatcaa taacatttga
     2401 atcaaaccta aatctgatta tttttgcagg aactcctgca actatggcat atgggggcac
     2461 atctttggta acaacggaat tggcagcaat aacagcacca tcacctattg cgatgccacc
     2521 tttcaataca acatcatttc ctatccagac atcatttcct ataatcggat tttcaggttt
     2581 ggtaatgaat ggtttcaatg tccagctacg ttcaaattca ctcatagcaa acttatcaaa
     2641 ttcaccgtta taagtcatca tgtgagtagt aaaacgattc aaaggatgat ttccacccat
     2701 tatttttaca tttgatgcaa tactacaaaa tcgacctata cgtatattcc ctgaaaaatg
     2761 gcaggtcgga aatgaaaaag cgccgacact ggagaaattt ttcacaggca ttgttgaata
     2821 ttcttcagca ataacattat cttcaattgt tatcttgtca ccttttgaga aaagaaataa
     2881 attcctttct ttatacgact tcttcccctt atgatttaaa aagattttat tatccataca
     2941 aaactcaaag tgttttttcg tccatctgaa ggtaagggtt ttatttttat cttccgtgaa
     3001 tttgtacaat tgaactttca ttgttaatac ttctacttat aaagtattag tttaataacg
     3061 atactctaaa gccttatgct aatcaactgt gacaggatga aaaatcgcca tcaaacattt
     3121 agcctaattt ttacgccagt tattaatgta atcattctaa tacttaaccg ccattaaact
     3181 cagaacctaa aacgatagtg gccgtcctat aacagccact aacatctaac ttaatgttta
     3241 accatgacgt ggcggacgac ggtgtaatcc tccagcccat aaagtgacat atccttgccg
     3301 taaccagaaa gtttctgccc accgtgcggc atttcactta ccagcatgaa atgggtattg
     3361 acccaggtac aaccatattg cagccgtgcg ctgacgcgat gcgccctgcc cacatctttc
     3421 gtccataccg aagatgcaag tccgtactgg ctgtcattcg cccagttcac cacctgttct
     3481 tcgttgtcga agggcgtaac actcactact ggaccaaata cctctttttg cacgatggca
     3541 tcgtcctgta atgcgccagc cagcagcgtc ggcgcatagt aatagccatt acccttgcgc
     3601 ttttcaccgc cagtgatcac tttgatgtgc cctgtcgctt tcgcctcttc tactgccttg
     3661 ctgacgcgtt cgagatgcgc cagcgagctt aaaggtccaa gctccgtaga ctcgtcatcc
     3721 ggcgcaccag attttaacgt tgccaccgca gcacccagtt tttccaccag cgtatcgtaa
     3781 atgccttttt gcgcgtagat ccgacaagcc gcagtacaat cctgtccagc attgtaatag
     3841 ccaaatgtac gtacaccttc gaccactgct tcaatatccg catcatcaaa aacaatcact
     3901 ggcgctttgc caccaagttc catatgagta cgcttaatgg acgacgcggt atggctgatg
     3961 atgtgctcgc cggtggcgat agagcccgtc agcgacacca tccgcacttt gggatgaccg
     4021 gtcagcggat cacccaccgt tttgcctctg ccaaacagta tgttaatcac gcctgccggg
     4081 aagatatctt tcgccagctc tgccaacttc aacgcggtca gcggggtaat ttctgatggt
     4141 ttaagcacta cgcagttccc tgccgccagc gccggagcaa gtttccacgc ggccatcatc
     4201 agcggataat tccacggtgc gatagaagcc acgaccccca acggatcgcg acggatcatc
     4261 gaagtatgac cttcaagata ttcacctgcc gccagaccat tcagacagcg cgccgcaccc
     4321 gcgaaaaagc gaaaaacatc gacaatcgcc gggatttcat cattgaacgc actatgcagc
     4381 ggtttgccac aattacggga ttccagttcg gcaaaaacct gaccattttc ttcgataaca
     4441 tccgccagtt tcagcaggca ttccgcacgc gctttcggtg tggtttgccc ccattcagca
     4501 aatgctgcat ctgccgcacg cacagcagca tcgacctgct ctgcggatac ctcggcaatc
     4561 tccagtaaaa cgtcccccgt tgccggatta tagacaggct gtttttcccc ttcgccgcta
     4621 acccgttctc cgttaatcag taacttatgt tgcatagctt attcctgtat cagtggttat
     4681 tgaccattgt cgccttcgcg ggttagccac caggccccca ggatcggcaa ggttgttacc
     4741 aacataacca gcagtgccac cacgttggtt accggtacat cacgcggtcg cccaagctga
     4801 ttgagcaacc acaacggtaa cgttcgttca tgacctgccg taaaggtcgt aacgatgatt
     4861 tcatcgaacg acaaggcaaa cgccagcatt cctcctgcca gtaacgccga actgagattc
     4921 ggcaacacta cgtagcggaa ggtttgccag ccattggccc caagatccat tgacgcctca
     4981 accagactcc acgaggtgcg gcgaaaacgg gcgatgacat tgttaaacac caccactacg
     5041 caaaaagtcg catgaccgac cacgatggtg aaaaatcccg gctccagatt gatggtttta
     5101 aaggcggtta atagcgccag accagtgaca atgcccggca gcgcaatggg cagcagcaat
     5161 aacagcgaaa tggcgttttt gccgaaaaag tctcgtctcc acagcgcggc agctgccagc
     5221 gtccctaaca ccagcgcaat taatgtcgcc agcgccgcca ctttaagtga cagcgtcacg
     5281 gcatcaagaa tatcactacg ctgtgctgcc acgctaaacc agcgcagcgt caggccctgc
     5341 ggtggaaaac taaacgccgc atcttcagtg ttaaacgcat aggcggcgat tatcaggatg
     5401 ggaaaatgta ggaaaacaac gccgccccag gccgccagtt tgaggaaaaa cggtgcgcgt
     5461 tcagagtgca tcgaacgctc ccagacgttt cacgaacgcc aggtacagtg cgatgagaat
     5521 aatcggcacc agggtgaatg ccgccgccat cggcatattg ccaatcgccc cctgctggga
     5581 ataaaccata ttgccgataa aatatcctgg aggaccaacc agctgcggga cgataaaatc
     5641 gcccagtgtg agtgagaagg taaagataga gccagcggca atacccggga ttgccagcgg
     5701 cagcaccaca tagcgaaagg tttgtcgtgg acgtgcgccg agatcagccg acgcctgcaa
     5761 caatgacggc ggcaaacgct caagcgccgc ctgaacgggc aggatcatcg agtaggcagc
     5821 ctgacggctg cggcttgtca tggtctgcaa ttaccgttat aaaaacaggc aatgtcattg
     5881 tctttcaggt ggttatatgg cccgttcagc taaaccccgt aaacgtaaac ccgcctctca
     5941 aagttcctga gtcgtgaccc gttcgaatgc gtgctgtgtg gcggccggat ggtataccgc
     6001 cgggccattg cggg
//
LOCUS       DAEBXN010000184         5983 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.185, whole genome shotgun sequence.
ACCESSION   DAEBXN010000184 DAEBXN010000000
VERSION     DAEBXN010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5983
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.185"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(1..393)
                     /locus_tag="KJL99_004028"
                     /pseudo
     CDS             complement(1..393)
                     /locus_tag="KJL99_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035092.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            complement(579..3887)
                     /gene="icsA"
                     /locus_tag="KJL99_004029"
                     /gene_synonym="virG"
     CDS             complement(579..3887)
                     /gene="icsA"
                     /locus_tag="KJL99_004029"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HBD4438568.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFATPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSNPSHAS
                     RTKLRINSDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGSGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSVINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVAGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGFS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGKGGILSLGAVLGNDNSKTDRLEIAGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQDEKERTGL
                     YMDAWLQYSWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTAHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGGNNIQAKLGLRTSLISQSCIDKETLRKFEPF
                     LEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            4416..5618
                     /gene="virA"
                     /locus_tag="KJL99_004030"
     CDS             4416..5618
                     /gene="virA"
                     /locus_tag="KJL99_004030"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HBD4438569.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDILLKIITFG
                     IYSPHETLAEKHSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPNDDYFHVKDTDFNSVWHDIYRDIRASDSNSTKIYFNNIEIPLKLIADLINE
                     LGINEFIDSKKELQMLSYNQVNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTS
                     AFWQSLHITSSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKNDTAI
                     SASGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVS
                     FAIISPDV"
     gene            5797..>5983
                     /locus_tag="KJL99_004031"
     CDS             5797..>5983
                     /locus_tag="KJL99_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBD4438570.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQGEYDSQWAAICSIAPKIGCIL
                     ETLRVWVRQHERDTGGGE"
BASE COUNT         1840 a         1355 c         1018 g         1770 t
ORIGIN      
        1 tcaggctgta cgcttcccca tgtgatttgc ctcgccactt caccgggctc aggaccccgt
       61 aggatcttca ccgaaaaaaa tgcatcacca gttgctttat tatttgtttt aggtagacct
      121 gttactaatt tatacctact ctgaccaagt ctggataaga aaggcacacc tgcacatttc
      181 tacttggaaa tgttttcttt taccattttg ctcaaaaatt tcaacatcca atattcctcg
      241 tacagaacta ctcaagtcct gaatagaaaa agttccagct ggtacagtag agtcatatat
      301 tactcgacct tgttgagata caacaacacg tgcattagtt tctgcaatac cagtaatctg
      361 aggcgcataa ccacgaatat ttggcggtag cattcgttca tcgctttcta atgcaattcc
      421 tgtgtaacgc caagcaacag cgcattctgg cgcatgatct ggtaaacacg tttggcattg
      481 atcgcaggca taccatcatg tgcacaaaaa cccaccgagc ttttacacgg tgggtcccag
      541 agaaatgcag gacatcaaca cgccctgcat ttttattatc agaaggtata tttcacaccc
      601 aaaatacctt gggtgtctct gtaactgtta ttacccaatt gctgagatac gtttccccag
      661 atgcttaggt tatctgcaag acgccccccc acaccagttt tgagttcaat cacattacgg
      721 ttgcctatct ggtgatttga catgccattc ataattacac catattgctt tgagctccat
      781 ttccaattca cttccaaaaa tggttcgaac ttacgaagag tctccttatc gatacaactc
      841 tgactaatta aggatgtacg caatcccaat tttgcttgaa tattattgcc cccagaggat
      901 gtgaccatag tgccattgtg ttcaatatag tcgtttgctt ttaccccatg cctagttatc
      961 tggacttgtg gattgagata caatgcgttg tcaatattat tatgagcagt ccagcgtatg
     1021 gttgggtaga tatagccagc ttccaaagct cctgttattc ctttgctgga atatttctca
     1081 ccagttaacc catctccttt gactgtatta ttaaaccaac tgtactgcaa ccaagcatcc
     1141 atatatagac ctgttcgttc tttttcatcc tgaaaccaag aactgtacaa accggcagta
     1201 taaccgctca cagttcctcg tgaactataa ctagtatact tattatgagt tttgctatcc
     1261 tgatatccat aagctcccat aatacccaca tgcaagccat catgatcagt gaagtttgtc
     1321 ttcaaaatat ctccaccaat ctgataaacc atcctgttag ctgtggtttt taattgtcta
     1381 tcagctaaat tatgctcatt atgtccacca acagtacgaa tccacatggt ggtttcgctg
     1441 agctgacctg tagtaggatc aacaatagtg ctaccgtgtc ggtcatagag agaaggacta
     1501 aacatcgtgt tcattgctat caggttagca atataactgc cagcttcagg gcgccatgtg
     1561 tgaatactac cttcacccaa attaagtgaa gatacaagct gtgaactagc tcggcgacta
     1621 ctcatttgag tagactcttg attgctcatc tgttttgatt cttgattatc catctgacta
     1681 gttagatacc acttattggt attcagtcca gatacagtgc cctgtttcag gcgatagtca
     1741 taactaccag caacaatacg gcctttctga ataaaagcat tcttatcaga agaatctgtc
     1801 gaaataattt gaacaccttc aagagtttta tctccagagc ctccctcatt tgtcactgca
     1861 acataggtaa taccagacgc atggcctgca atttccagcc ggtcagtttt actgttgtca
     1921 ttgccgagaa ccgcgcccaa agataatatt ccgccctttc catgccaatt acctttctga
     1981 acataggtct gaccgacatt tgacgaacta ttattgagaa taacatgacc gtttttctcc
     2041 agagtcatgt tacctgtgac aatgacgggg tcaggcaaaa aaggggcatt aatattattg
     2101 attagcacag tcccactatt taataaagtg ttaacagttt ggttcatgcc tgaaaaattc
     2161 aaggtagcac ctttattaac aataacacca gcggtacgtg tcatagcttc aactgtcccc
     2221 atttttagaa tgcctgcatt gatgttggtg aaaccagagt aggtattttt ttccgccaaa
     2281 atgagagttc cctcccctga ttttgttagg gattttccgt cccagctatt tatattatga
     2341 tggctgagat tatctgccag aatagtacta acagtaagtt cagcgttttc tttcagattg
     2401 aactctccat gactgtcgcc atctgtatca ttccagctca aaccgtagat caatttattc
     2461 tggtcactga tatagctttt tgtatagtca atagcaccag tagtgagtaa cggattgatg
     2521 atagatatat tagaaacact gaaacctgtt atccctttat ctgcacttag cagtttatag
     2581 tccttctgcg cccagttatt tataatagcc tccactttta cattgttgcc aaatgacata
     2641 tttccagaag cttccagact actgattcca gctaaattta aagaactgtt ctcaccaaaa
     2701 gaaacgtcac ctgaaacagt gaatttgtta cctgatagtg aaacagttgt acaatcacca
     2761 gcacataaat caccttcaat agttggtgtg gtcccagtgt aatcattgtt gataattttc
     2821 agaatggatg aattgttaac ctgtacatca ccagtaatga cagaaccttc atggagtatt
     2881 aatgaattgt ttccaccagt gatgtggata gcattacctg ctacattttg tgttccatca
     2941 tcttctttac ctgaaattat atatccgcta ttagcaatgg tcatattgct accatttata
     3001 gcatcccccc aatgtccacc attaccgcct aaaatagctc cattgttgat tacagacagg
     3061 ttatctcctg tgatagcatt accaccgtag ccatcataac cataaccgta agcagcacct
     3121 ccgttgcctc cagcgaacgt gcccttattg attacagaga ggctgcttcc tgtgatggca
     3181 tcacctccat taccgccatc accctcacca taattgttac ctccattacc accggaaata
     3241 gtccctttgt tgattatggg taaattgact cctgtgacgg catcacctcc attcccccca
     3301 tcactatcac tataactatc gccaccatgt ccacctgaaa taatttctcc atttattata
     3361 aacagattgt ctcctgtaac agcctcacca ccatcaccgt tatggtcagc accgctaccg
     3421 ccgctaccac caagaatcat gccttgattg attatagaca agtcagatcc ggtaatagag
     3481 tcaccaccat taccgccaca actattacca tcattattat caccagcacc accatgacct
     3541 gcgctaatca taatatcaga gttaatccgt agcttagttc tagatgcatg agaggggttg
     3601 gaagaagtta tataccacgc atccattcca tcttcaccag ctcccaaggg agaaagtcca
     3661 tcaacaggtg ttaataattt ttcataattg tcctctgaaa aatgaagttc ttgagtaccc
     3721 gaaagaggag tagcaaaagc tattggcccc ccgagcaaca gggatgcacc aacaacaaaa
     3781 ggagaaaaag acaatttgca tgttttttcc tttaccgtag gtaattctcc ggcccccccc
     3841 tgtgaacatt gggtcatatt acaaaaaaat ttgtgaattt gattcatgca ctatattatc
     3901 agtaagtggt tgataaaccc ctgaaaagat tccgttttat ccggaataaa gggacgatat
     3961 atgcaaaaac atattaaaca aagcctcaac caaaacatga gagctgtgtt atatatcaat
     4021 gcaacgggag aaatttacct gtgtcttgct ttgaaaaaaa gagggggaaa gctatatgtt
     4081 cgataataaa ttaataattt tactatgata gtctgcttca gaccgcaact tttcagttct
     4141 ttctgtacgc ttgcccaaag tttaaccact ttgtttttct atacacccaa tggttctatc
     4201 tgccccttga tgttaatccc caaagccaat aacatgaatg tattcatatc acaactttcc
     4261 taccagactc tccaatgcaa taactgcata attgttttta gtggaaatgt atcgttttct
     4321 taaagagaag aataacattc cattttatta tgttcccaat ttaattatat taatatatat
     4381 tggcattaat aggaaaatac atcaggagaa atcaaatgca gacatcaaac ataactaacc
     4441 atgaaagaaa tgactcatca tggatgtcaa ctgtcaaatc aactacagaa gtaagttgga
     4501 ataaattgag tttttgtgac attttactga aaataataac tttcggcata tattctccac
     4561 acgaaactct agccgaaaaa catagcgaaa aaaaactaat ggatagtttc tcaccatcac
     4621 tatcacaaga taaaatggac ggagagtttg cccacgcaaa catagatggc atttcaatac
     4681 ggctatgcct taacaaaggc atatgttcgg ttttttatct tgatggagat aaaatacaat
     4741 ccactcaact ttcatcaaaa gaatataata acttgttaag ttcactcccc cccaaacaat
     4801 tcaacttagg aaaagtacat actattacag ctccggtatc agggaatttt aaaactcaca
     4861 agcctgcacc agaagttatt gaaacagcca ttaattgctg tacatcgata ataccaaacg
     4921 atgattactt ccacgttaaa gacactgatt ttaatagtgt ttggcatgat atttacagag
     4981 atattcgagc atctgacagc aatagtacaa aaatatattt caacaatatt gaaataccac
     5041 taaaattaat tgcagatcta attaatgagt taggaattaa tgaattcatt gatagcaaaa
     5101 aagagcttca aatgctaagt tacaaccaag taaataaaat aatcaattca aacttccccc
     5161 aacaagacct atgttttcaa acagaaaaac ttttgtttac ttcactcttt caagaccctg
     5221 catttatatc tgcactaaca tctgcattct ggcaatctct tcacatcacg tcttcctctg
     5281 tggaacacat atatgcccaa attatgtccg aaaacataga aaacaggctt aatttcatgc
     5341 ctgaacaacg agttattaac aattgtggac atattataaa aatcaacgca gttgtcccta
     5401 aaaacgacac tgcaatctct gcctctgggg ggagggctta cgaagttagc tcatcaatat
     5461 taccatcgca cataacatgt aatggtgttg gtattaataa aatagaaaca agctatctcg
     5521 ttcatgctgg aacacttcca agcagtgaag ggcttcgcaa tgctatccct ccagaaagtc
     5581 gtcaagtatc atttgccatc atatctcctg atgtttaagc caatattata ttggctgcca
     5641 gactgtaaaa aaacgttctt cagaacagct ctataaacta ttaaaccaaa gctaaaagga
     5701 tggggaccaa tagaatctca gcccatcctt tttatatcat tatgaaccgc cccggaaatc
     5761 ctggagacta aactccctga gaaagaggta aacaggatga ctaaaaatac acgtttttcc
     5821 cctgaagtcc gtcaacgggc agttcgtatg gttctggaaa gtcagggcga atatgactca
     5881 caatgggcag cgatttgttc cattgcccca aagattggct gtatactgga aactctgcgt
     5941 gtctgggtac gccagcatga gcgggatacc gggggcggtg agg
//
LOCUS       DAEBXN010000185         5744 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.186, whole genome shotgun sequence.
ACCESSION   DAEBXN010000185 DAEBXN010000000
VERSION     DAEBXN010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5744
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.186"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            1..173
                     /locus_tag="KJL99_004032"
                     /pseudo
     CDS             1..173
                     /locus_tag="KJL99_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            698..1876
                     /gene="ltrA"
                     /locus_tag="KJL99_004033"
     CDS             698..1876
                     /gene="ltrA"
                     /locus_tag="KJL99_004033"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBD4438571.1"
                     /translation="MCVATREPPGCDGQTLKMFDQQRDGNLYKIWNRLCSGTWFPPPV
                     LEKRIPKSNGKERILGIPTVSDRIAQGAIKLFMEEKLDPIFHADSYGYRPGKSAHDAL
                     KQCAIRCWRYSWILEVDISAFFDHVRHDLVLKALEHHGMPKWVILYCRRWMEAPMQSC
                     ENGELITRTRGTPQGGVISPLLANLFLHYAFDLWMEREYRGVPFERYADDIVVHCSRM
                     SDATRLKNRLSERFSEVGLVLNAGKTNIAYIDTFKRRNVATSFTFLGYDFKVRTLKNF
                     NGERYRKCMPGASNAAMRKITETIKKWRIHRSTAESLLDFARRYNAIVRGWIEYYGKF
                     WSRNFNYRLWSAMQSRLLKWMQSKYRLSNRKAQRKLTLVRKEYPKLFVHWYLLRASNE
                     "
     gene            1959..2138
                     /locus_tag="KJL99_004034"
                     /pseudo
     CDS             1959..2138
                     /locus_tag="KJL99_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2168..2650)
                     /locus_tag="KJL99_004035"
                     /pseudo
     CDS             complement(2168..2650)
                     /locus_tag="KJL99_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016154415.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(2906..3886)
                     /locus_tag="KJL99_004036"
     CDS             complement(2906..3886)
                     /locus_tag="KJL99_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBD4438572.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(3886..5085)
                     /locus_tag="KJL99_004037"
     CDS             complement(3886..5085)
                     /locus_tag="KJL99_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBD4438573.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(5407..>5744)
                     /locus_tag="KJL99_004038"
     CDS             complement(5407..>5744)
                     /locus_tag="KJL99_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBD4438574.1"
                     /translation="HWLWYAYNTKTGGVLAYTFGPRNDETCRELLALLTPFCIGMVTS
                     DDWGSYAREVPKEKHLTGKIFTQRIARNNRTLRTRIKRLARKTICFSRSVEIHEKVIG
                     SFIEKHMFY"
BASE COUNT         1574 a         1317 c         1368 g         1485 t
ORIGIN      
        1 ttgctgaact gctggaaccg ctgtatgaca tcctgcgcca gtatgtgctg atgcccggta
       61 aagtccatgc cgatgatatc cccgttccgg tccaggagcc gggcagcggt aaaacaagga
      121 ctgcccggct gtgggtctac gttcgtgatg accgtaacgc cggttcggaa attgcagcat
      181 aagttatcca tgctctgagg tgggtagagg tgggccctcc ggtcagggtt tacaggagca
      241 cagaccaaac gacccggtgc tgcttcattc taaggaatga ggtggtgagc gactcgggaa
      301 aaggcagaga tctactgtca cgtgggttcg ggaagctgaa cgaaagtaaa ctgtccgatg
      361 aagcatcgaa atcttagatg atgccaaaat ggggccgtac agccgtcccc tgataagcgc
      421 agaagacacc tgcttactga ctgcgcggca tccggtgtga aggcagcagg atcccgggcc
      481 agaccatctc agggaacgtg agaacctgtt tccggcacgt atcgggaaac aggggcggac
      541 ccattcgtag tagtgatgaa agatctgtaa tgggtctgga gcgaagggat ggggatcaca
      601 ttcattgcag aagacacaac ccaaaagggg atgacgtaac tgatgcaacg aaaatcattt
      661 gaaataccaa aagcactggt ctgggcatca tatctggatg tgcgtcgcaa caagggagcc
      721 ccccggatgc gacgggcaga ctttgaaaat gttcgaccag caacgtgatg gcaatctata
      781 caagatctgg aaccggctat gttcgggaac atggtttccg ccaccagtgc tggaaaaacg
      841 gatcccgaag tcgaatggca aggaacgcat tctggggatc ccaacagtat ccgatcgaat
      901 tgctcaggga gcgataaaac ttttcatgga agaaaagctt gacccgattt tccatgctga
      961 ttcatatggt taccgaccag gcaaatcagc tcatgacgct ctgaaacaat gtgccatccg
     1021 gtgctggcgt tatagctgga tactggaagt ggacatcagt gcgtttttcg accatgtgag
     1081 acatgatctg gtgctcaaag ctctggaaca ccacgggatg cctaaatggg tcatcctgta
     1141 ctgtcgacgc tggatggaag cgccaatgca gagttgtgaa aatggagaat taataacccg
     1201 aacacgaggc actccgcagg gcggggtcat tagtccgcta cttgctaact tgttccttca
     1261 ctatgcattt gatttgtgga tggaaagaga atatcggggg gtaccgtttg agaggtacgc
     1321 tgacgatatt gtagtacatt gttcacgaat gagtgatgca acaaggctaa agaacaggtt
     1381 atcggagcgc ttctcggaag tcgggctggt cctgaatgca gggaaaacga atatcgccta
     1441 cattgacacg tttaaaagga gaaacgtcgc aacgagtttc acctttctcg gatatgactt
     1501 caaagtgcgt acgctgaaga atttcaacgg cgaacggtac cgaaaatgca tgccgggtgc
     1561 gtcaaatgca gcaatgcgca aaataacaga aacaatcaag aagtggcgta tacatcgctc
     1621 aacagctgag agtttgctgg attttgcgag gcgctacaat gcgatagtga gaggctggat
     1681 cgagtactac ggaaagttct ggtccagaaa tttcaactat cgactgtgga gtgcaatgca
     1741 gtcacgtctg ctcaagtgga tgcagtctaa atacagactt tcgaaccgga aggctcagcg
     1801 aaagctgacg ctggtaagga aggagtatcc gaagctattt gttcactggt atttactacg
     1861 tgcatcaaat gagtgatcaa gagccgtatg acgcgagagt gtcacgtacg gttccgtgag
     1921 cagccgggga gtgaaattct ctccggctga ctcgactcgc gggttccgac agcgatgacg
     1981 aacatgcggc ggtgttgtac tcgctgatcg gcacatgccg tctgaacaat gtggaaccag
     2041 aaaaatggct gcgttacgtc attgagcata tccaggactg gccggcaaac cgggtacgtg
     2101 atctgttgcc ctggaaagtt gatctgagct ctcagtaaat atcaatacgg ttctgacgag
     2161 ccgcttactt ttggcgtaat tctgctgcgc ctttactgaa cacaatgccg tattcagcaa
     2221 gaagtccacg aatgtgattt atcagttgtg tcctttccat tgaaaccaac cgacgcatat
     2281 tcaaaaaagt ctggacgtcc tgttgctcca gagtttttat tggtacgggt cgtatagttc
     2341 catgttggca ggcctcagct attgcctgtg cgtcgttata atcatttttc tggcaccgct
     2401 gataagcttt aacatgttgt gccggaagca atattacatc atggtcgagt ttcctgattt
     2461 cccgggccca gtaatgagat gccccgccag cctccatcgc aacagtcatt ttaggctgtt
     2521 gcaccagcca gttaagcagt tgattgcgag aaagtttggt ctttcctgcg ggtttgccat
     2581 gagcattgat actgaaaaga taaaagttcg ttttagccag atcaatgccg gtgattttct
     2641 gttcagacat ggtatcccgc ttacttttgt tgttaaagtt tatccatcat ggcgcaattg
     2701 aaggctgccg tgaaagaggg ggaagactat ctcatcggtt actgttaaac ggctaagaga
     2761 aactatcttt ttcaagcaaa gaagatacgg tgaaacaggc gatttcaccg tgaaatactg
     2821 ttcaaaaaaa acgctaataa aagcaaaatt tcttttaaaa cagccaatta aaaactggcg
     2881 agttgctggc gaaaaacttg aattttcaca gcgaattcag attatctttt agtacaagag
     2941 caatcatacg atctaactta tcctgaactt ccaacggtaa tctgccgaat tcatatgaga
     3001 acgtacgtcc ctttactcgt tttcgagcaa atttatcttt actatcaaat ttccataaca
     3061 aagttacaac cgctttatcc tttactcctg aatcaagcaa tgaagacatt tccttggtta
     3121 taagccgcat aatactgttt ttaacctcgt cttcagaaag attgtcatca gcagtaataa
     3181 gctcaatact cggcgagata ttcttcacaa cttcatcaat agatatatca cccagcctca
     3241 gtcgttcact taattcagcc agttgcctgt aatcggcgta tgtcagctca gactgaacag
     3301 gaaaaagaga cacaaaatct ttaggtacgg acgctgcttg gagggcacga gtcaccttag
     3361 ctgccgacaa tccctctctt tccgcaattt gcttctggtt cataccggcc tctttgagac
     3421 gtactaaacg caacccaatt tcacgaatat tatgttcaag tgacgtctgc aaatctttcg
     3481 ccagatgctg agcttcagaa acagtaagct catcatccgt tacaagtaca cgtaatccaa
     3541 ccttgcatag caaagctgct gcccgacgac gggaaccatc caaaatctca ataagatcgc
     3601 cagtccgaat cccaatacat ggatagaatt gttgtaaacg tatagttctg gtgatgtctt
     3661 ttagtgagtc cggcgtcagg gctgtctgtt cgcggccatt cacctcttca accacaaaag
     3721 tttgagtatc cactttgtca tgatcaacct tgatctcagt aaattttgcc tttcggccag
     3781 tattgagggt aaatacatga actggagccg atatttcttc agtattattc aggatatttg
     3841 tattcagcgt acggccaata gtcggtcggt gctttctgtt ttccatcagt agttggccct
     3901 tataaattca atacgatcaa aaactgcttt agcgaaatct tctgctgcca tacgtgcatt
     3961 tttcaaagct tcaccactgc caacataagt aacaggattc gcagatatca cggtatcaaa
     4021 cgactcgccg cttctctcaa aaccatcaag acgtggcatt gaaacatcaa gcatgtcacc
     4081 accgaaaatc tcttttgtca ggctatggca gtatttatga tctgacttat tcgccagttt
     4141 cgacatgaag ccaatatttg cctgcaaacg gcatgaacat ccatcctgtt caataatctg
     4201 tacaagttca ggtaaacgag ccaaatattt aagcgtggaa tggaaatcaa cttgtgctgg
     4261 cggcaccggt gtaaacatga tatctgcggc agcaatggca tttttcaaaa atgcgtccag
     4321 atgcgggcct gtatcaataa ggatgaagtc gaaatcatgc ttcagcttat caataatatt
     4381 ctcacgcaat atcgcgtgtt tgttttgtcc taatagatgc tcctcgcaca gagtatccca
     4441 gtttgatgca ataaaggcgt cgtcaataga agccggcata acataaacgc cagggataac
     4501 tgacggaacg ataaaatctt ccagtaattc ctcacgactc acgttctgca acatagcctg
     4561 cggtgcggtt gtatcaacca gccccaccgc atgcaagtag ttcagaaaca tagtggctga
     4621 tgactgggga tctaaatcaa ggagcagaat acgtaaatct tctgacagca ggtgaggatg
     4681 agcacgcagg gcgtgagcta cggaaacact actcacggtt ttagacacac cgccttttag
     4741 actaccgatg aatatggaat atgcctcact ataacgatcg cgatatttag gaatgccacg
     4801 atgtgcatag atatcgataa tgttctggat ggttaatgca tattttgtcg cggttcctgc
     4861 tgggcgtttt tcaaactgat agccctgagc ctccatctca ccgactgcac catccacgct
     4921 tgcgcgcgtt aattttggaa gcttagctac agcagacttt gaatagactt gataaaaaac
     4981 atcagcctga aactcctgct tttgctcctg aatctgtttt gttagggcga gcaacatttt
     5041 atctgcacgc tgcgcaacct tacttaactg ctcaaaacta gtcattcccc ctccgtgcta
     5101 tgtaggattt taacttatta caaaaaaaac tgcattagca gggtaatgca tgcaagtaca
     5161 tattgtacat gcttatatta aaaatgcgcg cattgctgat ttattaatca tcggaatgaa
     5221 tattactgta agatagtatg cctttccggg agaaagacgc tgatttcccc cgaataatcc
     5281 agttcaccac cacactccgg acaacagctt tcttcaggct ccagatggtg tatttcgcgg
     5341 gaaacatgtt ccggtaacgg acggcgatga cgtgactgtc gcaacaggcg ggggtagtgc
     5401 ttccaatcag tagaacatgt gcttttcaat gaaggagccg atgacttttt cgtggatttc
     5461 cacggaacgc gagaagcaga ttgttttacg tgccagccgc ttgatgcggg tgcggagggt
     5521 ccggttgtta cgtgcaatac gttgcgtgaa gattttgccg gtcaggtgtt tctccttcgg
     5581 cacttctctg gcgtagctgc cccagtcatc gctggtcacc atgccgatac agaacggagt
     5641 caacagggct aacagttccc gacaggtctc atcattccgt ggcccgaaag tgtaagccaa
     5701 cacaccgcca gttttagtgt tgtacgcata ccagagccag tgtt
//
LOCUS       DAEBXN010000186         5740 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.187, whole genome shotgun sequence.
ACCESSION   DAEBXN010000186 DAEBXN010000000
VERSION     DAEBXN010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5740
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.187"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..241
                     /locus_tag="KJL99_004039"
     CDS             <1..241
                     /locus_tag="KJL99_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438575.1"
                     /translation="FGIFYLVTRVCGFANGYFRVRTSEPVRGNVFLHNMTAFNKLAPR
                     TVGKREHKRGMYVYYCIIFCHRVILNGPARGPCGC"
     gene            complement(238..1179)
                     /locus_tag="KJL99_004040"
     CDS             complement(238..1179)
                     /locus_tag="KJL99_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HBD4438576.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLVGYTWSRDIQLRE
                     DASIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNPLNLWGME
                     LGWTVIELQAAQQVGRPIDTQKYDGMQLKWQMDNDEQVYVGDSALNLKGLVTLDGVPV
                     NNAAKTWATSTPDEIRASINQVLSDAWAASGYSVVPRDLLIPPEQFALLSSIIVSSAG
                     NQSLLTNLQTNTISYHQNGVPLNIRAVKWLKGRGVGNKDRIVAYTNDKKYVRYPLVPL
                     QSVPVQYRGLYQIVTYYGKLGAVEPVYKETISYVDGI"
     gene            complement(1191..1680)
                     /locus_tag="KJL99_004041"
                     /pseudo
     CDS             complement(1191..1680)
                     /locus_tag="KJL99_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1684..2904)
                     /locus_tag="KJL99_004042"
     CDS             complement(1684..2904)
                     /locus_tag="KJL99_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HBD4438577.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFVEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKVLAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            complement(2908..3651)
                     /locus_tag="KJL99_004043"
     CDS             complement(2908..3651)
                     /locus_tag="KJL99_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HBD4438578.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            complement(3542..5008)
                     /locus_tag="KJL99_004044"
     CDS             complement(3542..5008)
                     /locus_tag="KJL99_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HBD4438579.1"
                     /translation="MARNKQALRRTVQATADGYENFIARVGMQTPNQHSASTYRANFT
                     SRNRMLVEWSYRSSWIIGEAVDAIPDDMTRKGIRITSEIDAKDRGILESQLDELQIWD
                     ALNDVLKWSRLYGGAVGFIMIEGQAPMTPLRPETIGKGKFKGILPLDRWMVDPALTRR
                     IKDMGPDLGKPEFYDVVTTATGIPAWRIHHSRLIRFDGVTLPFQQKMTENEWGMSVVE
                     RIWDRLTAFDSATVGAAQLVYKAHLRTYSVEKLRELIALGGPAYEALLKNIDLIRQFQ
                     SNEGMTLMDSRDKFETHQYSFSGLDDILSQFAEQISGAVGIPLVRLFGQSPKGFSTGD
                     ADLANYYDRISSLQERRLRLPVRRILDIMHRSELGKPLPDDFTFEFNPLWQMSDVDRS
                     TVALNTTNAISTALGDGLMTLKAAMTDLRENSDVTGIGASITNEDIENAEDEAPPGIG
                     EPDDEPQEPSGGNPVSNQPTQDSEGGRRHRKWSLRWFK"
     gene            complement(5008..5241)
                     /locus_tag="KJL99_004045"
     CDS             complement(5008..5241)
                     /locus_tag="KJL99_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HBD4438580.1"
                     /translation="MDELISIDSRCPLLEKLKLELTTPHRDFDRNGRVMVESKKDLAK
                     REIPSPNVADAFIMAFAPIDTSLDIWEQLGRQA"
     gene            complement(5229..5708)
                     /locus_tag="KJL99_004046"
     CDS             complement(5229..5708)
                     /locus_tag="KJL99_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438581.1"
                     /translation="MDAHKTLNFEPSGRKRIGFDVADSGTDKCANVYRHGSVVFWADE
                     WKAKEDELLKSCQRTYQAALEREADIVYDSIGVGASAGAKFSEINADRKSENAYARRV
                     NYQRFNAGAGVHEPDDEYNGIPNKDFFANLKAQAWWLTVSEIRLTPLTTENSILWMS"
BASE COUNT         1225 a         1618 c         1437 g         1460 t
ORIGIN      
        1 ctttggcatt ttttacctcg ttacgcgcgt ctgtggcttt gccaacggat acttccgcgt
       61 gcgcacgagt gaaccagtgc gtggcaacgt cttcctccac aacatgacgg cctttaacaa
      121 actcgcgccg agaaccgtcg ggaagcgtga gcacaaacgg ggtatgtacg tgtattactg
      181 catcattttt tgccatcggg tcatccttaa tggccccgcc agggggccat gtggctgtta
      241 aatgccatca acgtacgaaa tggtttcttt gtacactggc tcgactgcac ccagcttgcc
      301 gtagtaagtg acgatctgat acagaccgcg atactgcacc ggcacgctct gaagcggaac
      361 cagcgggtag cggacgtatt ttttatcgtt ggtgtacgca actatgcgat ccttattccc
      421 cacaccacgg cctttcagcc atttaaccgc gcggatattc agcggaacac cgttctggtg
      481 atagctgatg gtgttggtct gaaggttcgt caacagggac tggttacctg cagatgaaac
      541 gatgatgctg gacaacagag caaactgctc aggcgggatc agcaaatcac gcggaaccac
      601 agagtaacca gaagcggccc acgcatcaga cagcacctgg ttaatgcttg cgcggatttc
      661 gtccggtgtt gaggttgccc acgttttggc agcgttgttg acaggcacgc cgtccagggt
      721 aacaaggcct ttcaggttta atgcggaatc gccaacatat acctgttcat cgttatccat
      781 ctgccatttc agttgcatac cgtcatactt ctgcgtatca atcgggcggc cgacctgctg
      841 agcagcctgc aattctatga ccgtccagcc aagttccatc ccccacaggt tcagcgggtt
      901 accggatttg ccgatatcca cgttcacgcc agcaatagcg gttgagtctt tgcctaccca
      961 gttttttcca ttcggatttg cgccagtacc cgcagcggcg aagctggtat tcgtccagct
     1021 ggaaatgtca tctgcgatgg aggcgtcttc acgcagttga atatcgcggc tccaggtgta
     1081 ccccaccagt ggcaggttca gcgtctggtc gagtctctcc agctccccga tgagaaaggc
     1141 accagagctg tcaacggttg cctgatcaaa agtaatcatt cgtctgttcc ttaaatcttc
     1201 caggaaattt ctgcattgcc gtcagcatca ccggcacctg tgaattcagc gttggtcagc
     1261 accacgtttt tgccactgac tgacgtggac atgaatccac ccagaggcac tttgatggat
     1321 tcatcagtgg agatcgcctt ttttgatagt gctggcatca aaatcagaac cgagattaac
     1381 ggtcacgtag ccacgcttca tggcgtcgcc cgggaagttc ttgcctgttc ccacctggcg
     1441 aaccatgtcc ggctgcgacg tggtcggata agggcgcacg tagatcccct tcaccttgtc
     1501 tgcggtatca ccatctgcca gcggcacgaa aaaaccgtca tcatcgtatt taccagccag
     1561 gccataggca gcgaaggcgt tatcggattt aaggaccacc ggttcgacgg ttaagtcctg
     1621 cgggcgagag acagccccgg caatgccaac aggcatccgg tacagaaata cattattcat
     1681 tttttaccct ttacggtttg cccagaattc agcgttttgt ttgttcaggg aagcgatact
     1741 ggtcatgccc atgtttaggc gctgtgcatc gccggtggtg gcgcgggtgt ttcgcccttt
     1801 ggcaacctca gacacggcat taaacgccat gtcgaccgat tgtttcggta atttgcggat
     1861 atccgcatca ccgactatct ggcgaaccag cgttttgtca gcagaagcca gaacctcgcg
     1921 tttgaacgcg gtcggtttca tcttacggct cagatcgata cccgggacga taacttcggc
     1981 acgccaggct gagtcaccag taatcgtggt ttcctcttca tcgtcctcgc cgtcaccggt
     2041 cggattatcg tcaggcttat tatcgttatc gcccgtggca tttctttcca gcttagccag
     2101 cagggctttc agtaatgttt tgaggtcatc atcactgtcg ccggttggat ctccgcccat
     2161 ctctggtgct ttgtccggta gtggttgctg cggggacagg ttgatgttga gattaacgcc
     2221 ctgcggcaaa tccccctcat ctcctgtaac cgatgcggga gccgactcca ccagttcgtt
     2281 catggtgtcg gcatctcctg tcttgatggc cgcacgcatg cggttccacc agtttttctt
     2341 ttgatttgcc attgtgtctc tgtctccaat tgcacaacga tttccggctc tgcctttagg
     2401 gacaagagcc acatggtttc cggtaatatc gacctgctcg gctttacctg gctcggtctg
     2461 ctcgtactcc gcgtcatagc cgcacgacac ttcgcgcagg ccatcttcga taagctgaat
     2521 ggcgctttcg tctttgacga taaggtcagc cagcatcaaa tcagactgct cacccgtccc
     2581 gcgccggaca ttctggaggt gcccgacagc aagcactttc cagttctcgg gatttaccag
     2641 ccgcacattc ccgttttcat cttcaggatg caggatcgtg atgctcatcc cttcgaatga
     2701 ggcaagcgtg gccggatgga atacctgctc aggagaacgc gtgacgacta tttcaacgaa
     2761 cttatcgggt ttcagttttg gcaggtcatc agcaccatag agctgcttac ctgttcgtcc
     2821 tatcggcacg tctttgcaca gcaacgagcc gtcagccagg cggtatcggg tttccccgag
     2881 cctggtctca aaaaagtatt tcatgggtta tgctgccctt ttcattgcga atggaacgcg
     2941 aacaaacacg atctctttat agcagcggca gtttggcagc tcgcccgcgt ggccggtcat
     3001 gccgtccaaa gttggaggtg aatcccagcg gacgaattta cctttcatgt gatcgtggga
     3061 atctcggaca tcgccatcat cggctgtacg ccaaatataa ccatccgagc caatagctat
     3121 cgctcgggcc atatccagcg cgcccgttgc tcttcccagt tccgttcggg caatcagatc
     3181 ggccctagac ttagcaacat cgccggtaga tgcaatctct ttagcaaact gctccgcccg
     3241 accaccggtg acaacagcct ctatcgcttt gttctggatg tcgtacaccc tgtctgcggc
     3301 ctcgagaggc agagatttga tgtacttgac ctgttcggcg atgatggatt gcatcacctg
     3361 gcccacagga gcgctttcca caagattgcg gagctcgcga ctgatgttct tgctgtgttg
     3421 ccgccaaact ttctcgttct gccgggttag gtccgcagta aagttttccg cgacctttgt
     3481 cgcccagggg gtgatgattt cactgtaacg ctccagcgcc gcaataattt ccgtgatact
     3541 gtcatttgaa ccatcgtagc gaccatttac gatgtctccg accgccctcg ctatcctgcg
     3601 taggctggtt cgataccgga tttccgcctg acggctcctg cggttcgtca tcaggttcgc
     3661 cgatgccggg cggcgcttca tcttcggcat tctcgatgtc ctcgttggta atggatgccc
     3721 ctatgccggt tacgtcagaa ttttctcgca aatcagtcat agcggctttc agtgtcatca
     3781 gaccatcacc cagcgccgta ctgattgcgt tggtggtgtt taacgccacc gttgagcgat
     3841 cgacatcaga catttgccag agcgggttaa actcaaacgt gaaatcgtcc ggcagcggct
     3901 tgccaagttc cgaacgatgc atgatgtcca gtatccgccg caccggaaga cgtaaacgcc
     3961 tctcctgcaa cgaactgatg cgatcgtaat agttggcaag gtctgcatca ccggtagaaa
     4021 atcctttcgg ggactgtccg aacaaccgca ccagtgggat accaacagcg ccactaatct
     4081 gttctgcaaa ctgcgaaagg atgtcatcca gaccactgaa gctgtactga tgggtttcaa
     4141 acttatcccg cgagtccatg agcgtcatgc cttcattact ctggaactgt cgaatcaggt
     4201 cgatattctt cagcaacgct tcatacgcag gaccaccaag tgcgataagc tcgcgtagct
     4261 tctccacgct gtaggtacgc aaatgcgcct tgtagaccag ctgcgccgcg ccgacagtag
     4321 cgctgtcgaa cgcggtaaga cgatcccaga tacgctctac aaccgacatt ccccattcgt
     4381 tctcggtcat cttctgctga aatggcaacg tgacgccatc aaagcgaatc aggcgactgt
     4441 gatgaatgcg ccaggcagga attcccgttg ctgtggtcac cacatcgtaa aactcaggtt
     4501 tacccaggtc cggccccata tctttaatgc ggcgggtcag tgccgggtcg accatccagc
     4561 ggtcgagcgg gagaatcccc ttaaacttgc ccttaccgat ggtttcgggt cgcagcgggg
     4621 tcattggtgc ctgcccctca atcatgatga aacccaccgc gccgccgtag aggcgcgacc
     4681 atttcagcac gtcattcagc gcatcccaga tttgcaactc atccagttgt gattcgagaa
     4741 tgccacgatc ttttgcatca atttccgaag tgatgcgaat gcctttgcgg gtcatatcat
     4801 ccgggatagc atcgactgct tcgccgatga tccaggatga acgataggac cattccacca
     4861 gcatgcggtt acgactggtg aaattagccc ggtaggtgga tgctgagtgc tggttaggtg
     4921 tctgcatccc tactcgggca ataaaattct cataaccatc agctgtggcc tgcacagttc
     4981 gccgcagggc ttgtttgttt cgtgccatca ggcctgtctc cccagctgtt cccagatatc
     5041 cagcgatgta tcgattggcg cgaaggccat aatgaatgca tcagcaacgt ttggtgacgg
     5101 tatctcgcgt tttgcgaggt cttttttact ttcgaccatc acacgtccgt tacggtcgaa
     5161 atcacgatga ggtgttgtca gttccagttt cagcttttca agcaacggac aacgagaatc
     5221 tatgctgatc agctcatcca caggatactg ttctccgttg ttaatggcgt taaacgtatt
     5281 tctgaaacgg tcagccacca tgcctgagcc ttaagatttg cgaaaaagtc tttgttgggg
     5341 atgccgttgt actcgtcatc tggctcatgc acaccagcac cggcgttaaa cctctggtaa
     5401 ttcacacgtc gcgcgtatgc gttctcgctc ttccggtcag cgttaatttc agagaattta
     5461 gcaccggcag acgcaccaac accgatagag tcgtaaacaa tatctgcttc acgctccagc
     5521 gccgcctgat aagtacgctg gcagctcttc agtaattcat cttctttggc cttccattcg
     5581 tcggcccaga aaacaacgga tccgtgacgg taaacgttag cgcacttatc tgtaccactg
     5641 tcagccacgt caaagccaat acgctttctt ccactgggtt cgaaatttaa cgttttgtgc
     5701 gcatccactg cggcttctat ccaagacagt ttgatgattg
//
LOCUS       DAEBXN010000187         5716 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.188, whole genome shotgun sequence.
ACCESSION   DAEBXN010000187 DAEBXN010000000
VERSION     DAEBXN010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5716
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.188"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..297
                     /locus_tag="KJL99_004047"
     CDS             <1..297
                     /locus_tag="KJL99_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HBD4438582.1"
                     /translation="KKNKQRKEQKPFLIPLLNPKAYLFFAALIPTFIDNNTNITLNFF
                     ILGVLFIFISFLTDLIYIAISLTIRDKLTPSFSRYISICSSIFILGTGIYFIFT"
     gene            complement(355..1071)
                     /locus_tag="KJL99_004048"
     CDS             complement(355..1071)
                     /locus_tag="KJL99_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438583.1"
                     /translation="MQLDEHSRKIAALVAYNDKQIKEMNRLITLLNERVNDLNTFTST
                     LEREGKVVSGISYSFRDSVSNVKFYINKGMDEASCKLQKSVSDTLTSLLREKLSFTLN
                     DVKKEWVDFEEQTSKEIKESLKQTTREAEVATGIIRKTMEKYTVTRIVEKVIVLIVLF
                     LLLMIAPVFYAEYLKMNISSLMEEQKKHESMLKEWKEKAPLAEFSKCGDYRKACVLVD
                     DESGYYFNEQSPNKKYAILK"
     gene            complement(1092..2177)
                     /locus_tag="KJL99_004049"
     CDS             complement(1092..2177)
                     /locus_tag="KJL99_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HBD4438584.1"
                     /translation="MASYHCTVKTGAKGVALKHADYISRSGEYKNYKSREDLEFSSSG
                     NMPSWAKKNPAELWKAADEFERKNGTAYREIEIALPRELTREQRIELVEDFVQKELGD
                     RHAYQYAIHNPPGAIDGKEQPHAHIMFCERINDGIERDPQQFFKRANSKSPERGGAKK
                     ARFGETPQERKSALVALRSHWADVQNEHLARHGHQSRVDHRSLKEQGIKRTPEVHLGP
                     VRVAVMNADQVCAILARRNAEREKLEAEQERDSIIDLKTTLKEALAERDNIPPSLDEL
                     GAELKAFVESSFDELNEVMQLNSLINGALDSMMKQFQVEEERKLQQERLIEAEKQRDL
                     ERERERVRQRAYSSPAPSRSRDYGMSR"
     gene            2349..2795
                     /locus_tag="KJL99_004050"
     CDS             2349..2795
                     /locus_tag="KJL99_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HBD4438585.1"
                     /translation="MTNREEWLSAKIAYINGLKSPSEQQRLLVLLAEKKNRTTTDEKT
                     LSALIRAEKTAEKAAAAKARVTAIIAAERKAAARAERKARDHELYKAAGLMIVAGLVD
                     SKTGKPKFSAAELVGALAGIAELPRNHPKWQEWEKRGKELLTKDSA"
     gene            complement(3002..3931)
                     /locus_tag="KJL99_004051"
     CDS             complement(3002..3931)
                     /locus_tag="KJL99_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBD4438586.1"
                     /translation="MSAVLQLFREKLPHKPYCTNDFTYGVRILPKDIAILARFIQQNQ
                     PHAQHWLAFDVDRTGAAIDWSDRNCPAPNITVKNPRNGHAHLLYALTLPVRTAPDASA
                     SALKYAAVIERALCEKLGADVNYSGLICKNPFHQEWQVVEWRWELYTLDELADYLDLS
                     ASARRRVDKDYGMGRNWYLFEKGRKWSYRAIRQGWPAFSPWLDAVIQRVEMYNASLPV
                     PLSPAECRAIGKSIAKYTYRKFSPEGFSAVQAARGRKGGNANSSENQAIKGQKSKRVA
                     VATSARTLKPWVTLGISRATYYRKLKCDPDLAK"
     gene            4484..5434
                     /locus_tag="KJL99_004052"
     CDS             4484..5434
                     /locus_tag="KJL99_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438587.1"
                     /translation="MNNFINECISAPRINGMAEFFSSTASGEKHKPFRLVEIDDRLFH
                     SYDYIKFAKHLTKNSGPFVNHYFCSIPYSKEEECRLGTTVIKYAITLNKKLNTWCLGM
                     AEGAMARTISELSKGNIQTLTTSPTKENEHAFFKYGSSENAYFICTPFFLLEERLKNS
                     NLKQFNQGFDIIIEDTTFQMYSPNRSGQINHVKRLLNNDGIFIFTEKHSSEHDEYQKR
                     EIQKDYSFKQRYFSKDEINTKKEIVLNTMNLNEVSVDDMLSAIKQHFKYASIYWNSGN
                     FYSIAASNSLKHISTFINLLPKAAIPMEYVYEKLPRNISW"
     gene            5441..>5716
                     /locus_tag="KJL99_004053"
     CDS             5441..>5716
                     /locus_tag="KJL99_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HBD4438588.1"
                     /translation="MEYSTLLSFAIVTLSQTISIGPGVALVINNAFSHGLKSSIKTSI
                     YIRIGETIVMAISLFALSSTSSTEQHFHIIKIFGGGYLIYIGLMGLIN"
BASE COUNT         1713 a         1313 c         1108 g         1582 t
ORIGIN      
        1 aaaaaaaaca aacaaagaaa agagcaaaaa ccttttttga taccattatt aaaccccaag
       61 gcgtatcttt tctttgctgc gttaatacca acctttatag ataataatac aaatatcaca
      121 ttaaactttt ttatattggg tgttttattt atatttatat cattcctaac agatcttatt
      181 tacatagcta ttagcttaac gataagggat aaattgacgc catcattctc aagatacata
      241 agcatatgta gttccatttt tatattaggt acaggtattt attttatttt tacgtgaaac
      301 gccgttttta tactcatgca gccttagtca taagacggca tgagttactt aacactattt
      361 cagtatggca tattttttat ttggtgattg ttcattaaaa taatatccgc tttcgtcatc
      421 aactaacacg catgctttac gataatctcc acacttcgag aactcggcca atggagcttt
      481 ttctttccat tcttttaaca tggattcatg ttttttctgt tcttccatta gcgaactaat
      541 attcattttg agatattcag catagaaaac aggagcgatc attagcaata aaaataatac
      601 aatcaacaca attacctttt cgacaatcct tgttactgta tacttctcca ttgtttttct
      661 aattatacct gtcgcaactt cggcctctct ggtcgtctgc tttaatgatt cctttatctc
      721 tttacttgtt tgctcctcga aatcaaccca ctctttcttt acatcattga gagtgaaaga
      781 taatttttcg cggagtaatg acgttagtgt atctgagact gatttctgta atttacaaga
      841 tgcctcatcc attcctttat taatataaaa tttaacatta gacacactat ccctaaagga
      901 ataagaaatt ccagaaacaa ctttaccttc acgttcaaga gtacttgtaa aggtatttaa
      961 gtcattcact ctttcattaa gcagagtaat tagcctgttc atttctttta tttgtttatc
     1021 attatacgca acaagagcag caatttttct tgaatgttca tccaattgca taaaataaaa
     1081 acctccagtg cttaacggct cataccataa tcacgtgaac gtgatggtgc tggtgaggaa
     1141 tacgcacgct ggcgcactct ttctctttcc ctctccaggt ctctttgctt ttcggcttca
     1201 attagtcgtt cctgttgcaa cttccgctcc tcttccacct ggaattgctt catcattgag
     1261 tccagcgcac cgttaatcaa tgaattaagc tgcattacct cattgagttc atcaaacgag
     1321 ctttcaacaa acgccttcaa ttctgcccca agctcatcca ggcttggtgg tatgttatcc
     1381 cgttcagcta acgcttcctt caaggtcgtt ttaaggtcaa tgatgctgtc ccgttcctgc
     1441 tctgcttcta gcttctcccg ttccgcgttt ctccgtgcca gaattgcaca aacctgatca
     1501 gcattcataa cagcaacacg aacgggacca agatgaactt ccggtgtgcg cttgataccc
     1561 tgttctttca gtgaacgatg atcaacgcgc gactgatggc cgtgccgcgc aaggtgttca
     1621 ttctgcacat cagcccagtg agaacgtagc gcaaccaagg ctgattttcg ctcttgtggt
     1681 gtctcaccaa agcgtgcctt ttttgcacct ccccgctccg gtgatttgct gttggcacgt
     1741 ttaaaaaact gctgcggatc gcgttcaata ccatcattaa tccgctcgca aaacatgata
     1801 tgtgcgtgtg gttgttcctt tccgtcgatt gcacctggcg gattgtggat cgcgtactga
     1861 taggcatggc gatcaccaag ctccttttgc acgaaatctt caacaagttc tatccgttgt
     1921 tcgcgtgtca gttctcgcgg taatgcgatc tcaatttcac ggtatgccgt cccgtttttt
     1981 cgttcaaatt catctgctgc tttccatagc tctgcgggat ttttcttcgc ccatgatggc
     2041 atgttaccgg atgacgaaaa ttccagatcc tcccgcgatt tgtagttctt atattcgccg
     2101 gatctagaaa tataatcagc atgttttaag gctacgcctt ttgctccagt tttaactgta
     2161 caatggtagc ttgccatctg ccgttaagcc ctccgcagga gcgcactgtt tctcgacgaa
     2221 ggagagaaac gtctaagtgc gcatccctga attaatcagg gatgatttta aggtctcaca
     2281 cgcaggcaga caaggtgata ttatgcacaa taacaactca cattaacggc aaaagtgcag
     2341 aggtaagaat gacaaatcgt gaagagtggt taagtgcaaa aattgcctat atcaacggac
     2401 tgaaatcacc gtcagagcag caaagattac ttgtgttact ggcagagaaa aaaaaccgaa
     2461 caacgacgga tgaaaaaacg ttgtccgcac tgatacgggc agaaaaaacc gcagagaaag
     2521 cagccgcagc aaaagccagg gtaacggcaa taatagcggc agaacggaag gcagccgcaa
     2581 gggcggaacg aaaggcgcgg gatcatgaac tctacaaggc cgcaggcctc atgattgtgg
     2641 caggactggt ggacagcaaa accggaaaac caaagtttag tgctgccgaa ctggtaggcg
     2701 cactcgcagg aatcgcagag ctaccgcgca accatccaaa atggcaggaa tgggaaaaac
     2761 gcgggaaaga gctactcacc aaagacagtg cgtaagagcc tccgttcacg ttccggttcc
     2821 ctggtgcttc ctcgctcatt cgctcgctac gctcggtcat tgagactgcg gcgagcacaa
     2881 ccaccactca cacacagacg ctaaaaccat tgccaaacga aacacgggaa atcaccccaa
     2941 acctacccgc tcaaaaaaac gcctacaggc gctgttatct gataaggctt gttatctggt
     3001 ctcattttgc gagatctgga tcacatttta gttttcgata gtatgtggca cgactgatcc
     3061 ctaacgtcac ccacggcttc agagttcgtg ccgatgtggc aacggctaca cgctttgatt
     3121 tctgaccttt aatagcctga ttttctgatg aatttgcatt accgccctta cgacctcgag
     3181 cagcctgtac cgcggaaaag ccttcaggag agaatttgcg gtaggtgtat ttcgcaatac
     3241 tcttgccaat agcccgacat tcagccggag aaagcggaac gggaagcgat gcgttgtaca
     3301 tttcgacacg ctgaatcact gcatcaagcc acggtgagaa tgctggccag ccctgacgaa
     3361 tcgccctgta agaccattta cggccctttt cgaacagata ccagtttcgc cccatcccgt
     3421 aatctttatc gacgcgacgg cgcgctgagg cgctcaaatc aagataatcc gccagttcgt
     3481 cgagggtata gagttcccaa cgccattcca ccacctgcca ttcctggtgg aacggatttt
     3541 tgcagatcag gccgctgtag ttcacatccg cgcccagttt ttcacacagc gcacgctcaa
     3601 tcacggcagc gtatttgagc gccgaagccg atgcatccgg cgcggttctc accggaagag
     3661 tgagcgcgta gagcagatgc gcgtgcccgt tacgtggatt ttttacggtg atgttcgggg
     3721 ccggacagtt ccggtcgctc cagtcgatcg ctgcccccgt ccggtcaacg tcaaaggcca
     3781 gccagtgctg cgcatgtggc tggttctgct ggatgaaacg ggcaaggatg gcgatatctt
     3841 tcggtagaat gcgaacgccg tacgtgaaat cgttcgtaca gtacggtttg tgcggtaatt
     3901 tttccctgaa gagttgaagt acggcgctca tgtgttagga tttcctccgt gtgttacgac
     3961 ccacacgccc cttgaatttt cgtgacagtc ttggcggatc ggcacgaatt tcaggggcgt
     4021 ttgtttttca gttggttaca ctacacccaa cggcaaatcc tgtcgatcct tcccgaaaac
     4081 cagacccatt taccataact cctcttgtac gtaccctcta aaacgctctc aatcgctttc
     4141 tgacgcaaaa aactcaaaaa gacaagataa acctagggaa aacacaaaaa acgcgtcaga
     4201 atcgttttta ggggcctgca taactatgca caaaagcgat taatgatcgg tttcacccct
     4261 tcagaagcgc gaactggtca aaacagcaaa ctttctcatc cagaaagcca gctgaactaa
     4321 cccgcatgaa aacaaatttc caaacatatc cagtctggac aacgaaatat cgacaatata
     4381 aaacatcata atcgtgttta tggcgtgtaa aaaagcttac aagagaatta gcattgaaga
     4441 gtcataacaa caaattccaa aaatcaaacc agaggatagg aaaatgaata attttataaa
     4501 tgaatgtatt tccgctccga gaataaacgg catggcagaa tttttctcat caactgcatc
     4561 aggggaaaaa cacaaacctt ttagactagt agaaatagat gatcggcttt ttcattctta
     4621 tgattacatt aagtttgcca aacacctcac aaaaaacagc ggccctttcg ttaatcatta
     4681 tttttgcagt ataccataca gcaaggaaga agagtgcaga ttaggaacaa cagtaataaa
     4741 atacgcaatc acgctaaaca aaaaattaaa cacatggtgt cttggtatgg ctgagggcgc
     4801 aatggcaaga accatttcag aattatcaaa aggaaacata caaacattaa caaccagtcc
     4861 aacaaaagaa aatgaacatg cattttttaa gtatggctca agtgaaaacg cttattttat
     4921 ttgcactcca tttttcctgt tagaggaaag actaaaaaat tcaaatctta aacaatttaa
     4981 tcaaggattc gatattataa tagaagacac cacatttcaa atgtactcac ctaacagatc
     5041 tgggcaaata aaccacgtaa aaaggctatt aaataatgat ggtattttca tatttacaga
     5101 aaaacatagc tcagaacatg atgaatatca aaaaagagaa atccaaaaag attattcatt
     5161 caaacaaagg tatttctcga aagatgagat taacaccaaa aaggaaatcg tactaaacac
     5221 aatgaatttg aatgaagtta gtgttgacga tatgctttcc gcaattaaac aacattttaa
     5281 atacgcatca atatattgga atagcggaaa tttttactca atcgccgcca gcaattcact
     5341 aaaacacata tcaacattta taaatttatt accgaaagct gcaattccta tggaatatgt
     5401 atatgaaaag ctcccacgaa atatatcatg gtaagtaaac atggaatatt caacattatt
     5461 atcatttgct atcgtaacct tatcgcaaac tatatcaata gggcctggcg ttgcattggt
     5521 tataaacaat gccttttcac atggattaaa gtcatcaata aaaacaagta tatacataag
     5581 aattggcgag actatcgtca tggcaatatc tcttttcgcg ttatcctcaa catccagcac
     5641 agaacaacat tttcacatta taaaaatatt cggtggaggc tatttaatat acataggatt
     5701 aatgggttta attaat
//
LOCUS       DAEBXN010000188         5702 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.189, whole genome shotgun sequence.
ACCESSION   DAEBXN010000188 DAEBXN010000000
VERSION     DAEBXN010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5702
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.189"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(<1..170)
                     /locus_tag="KJL99_004054"
     CDS             complement(<1..170)
                     /locus_tag="KJL99_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438589.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSIG
                     EPFFFRVIVPFK"
     gene            246..2807
                     /gene="mutS"
                     /locus_tag="KJL99_004055"
     CDS             246..2807
                     /gene="mutS"
                     /locus_tag="KJL99_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBD4438590.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRSNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            2913..3565
                     /gene="pphB"
                     /locus_tag="KJL99_004056"
                     /pseudo
     CDS             2913..3565
                     /gene="pphB"
                     /locus_tag="KJL99_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
     gene            complement(3616..4383)
                     /locus_tag="KJL99_004057"
     CDS             complement(3616..4383)
                     /locus_tag="KJL99_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBD4438591.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRINVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVLPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LCSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            4579..5487
                     /locus_tag="KJL99_004058"
                     /pseudo
     CDS             4579..5487
                     /locus_tag="KJL99_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
     gene            5484..>5702
                     /locus_tag="KJL99_004059"
     CDS             5484..>5702
                     /locus_tag="KJL99_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HBD4438592.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQ"
BASE COUNT         1410 a         1469 c         1481 g         1342 t
ORIGIN      
        1 atcttaaagg gcactatgac tctaaagaag aagggttcgc caatcgatac aattttgcgt
       61 acttgcttca taagcatcac gcaaaagctg caaaacagca tctttcccgg aaccagcatc
      121 aagaactcgc cgttcgcttc ttcccctgaa atgattaact ccggtatcat gtgcgcctta
      181 tgtgattaca acgaaaataa aaaccatcac accccattta atatcaggga accggacata
      241 accccatgag tgcaatagaa aatttcgacg cccatacgcc catgatgcag cagtatctca
      301 agctgaaagc ccagcatccc gagatcctgc tgttttaccg gatgggtgat ttttatgaac
      361 tgttttatga cgacgcaaaa cgcgcgtcgc aactgctgga tatttcactg accaaacgcg
      421 gtgcttcggc gggagagccg atcccgatgg cggggattcc ctaccatgcg gtggaaaact
      481 atctcgccaa actggtgaat cagggagagt ccgttgccat ctgcgaacaa attggcgatc
      541 cggcgaccag caaaggtccg gttgagcgca aagttgtgcg tatcgttacg ccaggcacca
      601 tcagcgatga agccctgtta caggagcgtc aggacaacct gctggcggct atctggcagg
      661 acagcaaagg tttcggctac gcgacgctgg atatcagctc cgggcgtttt cgcctgagcg
      721 aaccggccga ccgcgaaacg atggcggcag agctgcaacg ctctaatccg gcggaactgc
      781 tgtatgcaga agatttcgcc gagatgtcgc tgattgaagg ccgtcgcggc ctgcgccgtc
      841 gcccgctgtg ggagtttgaa atcgacaccg cgcgccagca gttgaatctg caatttggca
      901 cccgcgatct ggtcggtttt ggcgtcgaga acgcgccgcg cggactttgt gctgccggtt
      961 gtctgttgca gtatgcgaaa gatacccaac gcacgaccct gccgcatatt cgttctatca
     1021 ccatggaacg tgagcaggac agcatcatta tggatgccgc gacacgtcgt aacctggaaa
     1081 ttactcagaa cctggcgggc ggtgcggaaa atacgctggc ttctgtgctc gactgcaccg
     1141 tcacgccgat gggtagtcgt atgctgaaac gctggctgca tatgccagtg cgcgataccc
     1201 gcgtgttgct tgagcgccag caaactattg gcgcattgca ggatttcacc gccgagttgc
     1261 agccggtact gcgtcaggtc ggcgacctgg aacgtattct ggcacgtctg gctttacgaa
     1321 ctgctcgccc acgcgatctg gcccgtatgc gccacgcttt ccagcaactg ccggagctgc
     1381 gtgcgcagtt agaaactgtc gatagtgcac cggtacaggc gctacgtgag aagatgggcg
     1441 agtttgccga gctgcgcgat ctgctggagc gagcaatcat cgacacaccg ccggtgctgg
     1501 tacgcgacgg tggtgttatc gcatcgggct ataacgaaga gctggatgag tggcgcgcgc
     1561 tggctgacgg cgcgaccgat tatctggagc gtctggaagt ccgcgagcgt gaacgtaccg
     1621 gcctggacac gctaaaagtt ggctttaatg cggtgcacgg ctactacatt caaatcagcc
     1681 gtgggcaaag ccatctggca cccatcaact acatgcgtcg ccagacgctg aaaaacgccg
     1741 agcgctacat cattccagag ctaaaagagt acgaagataa agttctcacc tcaaaaggca
     1801 aagcactggc actggaaaaa cagctttatg aagagctgtt cgacctgctg ttgccgcatc
     1861 tggaagcgtt gcaacagagc gcgagcgcgc tggcggaact cgacgtgctg gttaacctgg
     1921 cggaacgggc ctataccctg aactacacct gcccgacctt cattgataaa ccgggcattc
     1981 gcattaccga aggtcgccat ccggtagttg aacaagtact gaatgagcca tttatcgcta
     2041 acccgctgaa tctgtcgccg cagcgccgta tgttgattat taccggcccg aacatgggcg
     2101 gtaaaagtac ctatatgcgc cagaccgcac tgattgcgct gatggcctac atcggcagct
     2161 atgtaccggc acaaaaagtc gagattggac ctatcgatcg catctttacc cgcgtaggcg
     2221 cggcagatga cctggcttcc gggcgctcaa cctttatggt ggagatgact gaaaccgcca
     2281 atattttaca taacgccacc gaatacagtc tggtgttaat ggatgagatc gggcgtggaa
     2341 cgtccaccta cgatggtctg tcgctggcgt gggcgtgcgc agaaaatctg gcgaataaga
     2401 ttaaggcatt gacgctgttt gccacccact atttcgagct gacccagcta ccggagaaaa
     2461 tggaaggcgt cgccaacgta catctcgatg cgctggagca cggcgacact atcgccttta
     2521 tgcacagcgt gcaggacggc gcggcgagca aaagctacgg tctggcggtt gctgctctgg
     2581 cgggcgtacc gaaagaggtt attaagcgcg cacggcaaaa actgcgtgag ctggaaagca
     2641 tttcgccgaa cgccgccgcg acgcaagtgg atggtacgca gatgtctttg ctgtcagtgc
     2701 cagaagaaac ttcgcctgcg gtcgaagcac tggaaaatct cgacccggat tcactgactc
     2761 cgcgtcaggc gctggagtgg atttatcgct tgaagagtct ggtgtaataa caattcccga
     2821 tagtcttttg ctatcgggaa tattaacgac aactgacgaa tcaaataaaa ataccctgta
     2881 taataggaaa gcttatttta cagggtaaaa ccatgccatc tacacgctac caaaaaatca
     2941 atgcccatca ctatcgccat atatgggtcg ttggtgatat tcatggtgaa tatcagttat
     3001 tacaatcccg cttacatcaa ctctcttttt tccccgaaac cgacttactt atttctgtcg
     3061 gcgataatat tgatcgtggg ccggagagtc ttgacgtcct gcgcctgcta aaccagccct
     3121 ggtttatctc cgttaaaggc aaccacgaag caatggcgct ggatgcgttc gcgaccggcg
     3181 atggcaatat gtggcttgcc agcggtggtg actggttttt cgatttaaat gattgcaaca
     3241 agaggcaata gttctgttgc tgaaattcca tcaccttcca catattattg aaatcactaa
     3301 cgacataata aaatatgtca tcgcacatgc agattatccg ggtgatgaat atctctttgg
     3361 caaagaaata gcggagagcg aattactctg gcctgtttat cgtgtgcaga aatcgcttaa
     3421 tggcgagtta caacaaataa acggcgctga ttattttata tttggacata tgatgtttga
     3481 taacattcag acgttcgcta accagattta tattgatacc ggatcgccga aaagtgggcg
     3541 gctgtcattt tataaaataa ggtagtctca tgcttcttct gtgaagcatg agtaaccaga
     3601 tgttatttca ggccattatt catttttcgc taccagcaaa gagagatcct gcttcgccag
     3661 cgcgcgactg gcactctccg gcaaaccgtc gtctgtaata atctgatcaa actcgcttaa
     3721 tggtaacgcc agccatgtcg ccacctgacc atatttcgtc gcatcacaga ccaaaactcg
     3781 ctggcggctg gcactggcaa tcgcccgttt caccgtgact ttatcttccg ctggcgtaga
     3841 aatcccccgc acactccatg acgatgcaga aataaaagcc tgatcaatca tcaggctgca
     3901 cagcatggtc gcagcggctt ccccgacaca ggaacggttt tcccgacaca ctgcaccgcc
     3961 agtgtgaata attgtgcaat tactgttgtc gagcaagtag ttcgcaataa cgaaatcgtt
     4021 tgtgaccaca gtcagtgact ccatgtgaat cagatgctgt gctatcgcta acgtggtcgt
     4081 tcccgcatcc agatagatac aacttcccgg cagaacaaga cttgccgcca gcttgccaat
     4141 agccgctttt tgcgtcattg ccagcgcagt ttttacctga tgagaaggtt catgcgccac
     4201 gcgtcccgga gactggacgc ctccggacac cagcacaacg gctccctgct gctccagttt
     4261 ttgtaaatcc cgacgaatgg tcatatgtga cacattaatt ctgtccgtta gttcagcaat
     4321 actgacaatg cctttttcag ctaccatctc aaggatgatt tggcgacgct ctacgggtat
     4381 caacttttgc tccttccttt gtcctgctga cattctacgc tatttgcctg cgaaacgtgc
     4441 gcggcgcaac taacgcttag ttcacataaa ataacacaaa atgttaattt atgtgaatca
     4501 tctcaccata tcgttatctc ccagcgctta tgttcacaat atcaaacaaa atatcactta
     4561 aattaacaag gagagcagat gaaaacggga tctgagtttc atgtcggtat cgttggctta
     4621 gggtcaatgg gaatgggagc agcactgtca tgtgtccgcg caggtttttc tacctggggc
     4681 gcagacctga acagcaatgc ctgcgctacg ttgaaagagg caggtgcttg cggggtttct
     4741 gataacgccg cgacgtttgc cgaaaaactg gacgctctgc tggtgctggt ggtcaatgcg
     4801 gcctaggtta aacaggtgct gtttggtgaa acaggcgttg cacaacatct gaaacccggt
     4861 acggcagtaa tggtttcttc cactatcgct agtgctgatg cgcaagaaat tgctaccgct
     4921 ctggctggat tcgatctgga aatgctggat gcgccagttt ctggtggtgc agtaaaagcc
     4981 gctaacggtg aaatgactgt catggcctcc ggtagcgata ttgcctttga acgactggta
     5041 cccgtgctgg aagccgttgc cggaaaagtt tatcgcatag gtgcagaacc gggactaggt
     5101 tcgaccgtaa aaattattca ccagttgtta gcgggcgtac atattgctgc cggagccgaa
     5161 gcgatggcac ttgcagcccg tgcggggatc ccgctggatg tgatgtatga cgtcgtgacc
     5221 aatgccgccg gaaattcctg gatgttcgaa aaccggatgc gtcatgtggt ggatggcgat
     5281 tacaccccgc attcagccgt cgatattttt gttaaggatc ttggtctggt tgccgataca
     5341 gccaaagccc tgcacttccc gctgccattg gcctcaacag cattgaatat gttcaccagc
     5401 gccagtaacg cgggttacgg gaaagaagac gatagcgcag ttatcaagat tttctctggc
     5461 atcactctac cgggagcgaa atcatgatca agattggcgt tatcgccgat gattttaccg
     5521 gcgcgacgga tatcgccagt tttctggtgg aaaacggtct accaacggta caaattaacg
     5581 gtgttccaac aggtaaaatg ccggaagcaa tcgacgcact ggtgatcagc ctgaaaacgc
     5641 gctcctgtcc ggtggttgaa gccacacagc aatcgctggc ggctctgagc tggttgcaac
     5701 ag
//
LOCUS       DAEBXN010000189         5654 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.190, whole genome shotgun sequence.
ACCESSION   DAEBXN010000189 DAEBXN010000000
VERSION     DAEBXN010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5654
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.190"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..411
                     /locus_tag="KJL99_004060"
     CDS             <1..411
                     /locus_tag="KJL99_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBD4438593.1"
                     /translation="AVRQQRSKALLTSLNEWMVEKNGTLSKKSRLGEAFSYVLNQWDA
                     LCYYSDDGLAEADNNTAERALRTVCLGKKNYMFFGSDHGGDRGALLYGLIGSCRLNGI
                     DPEAYLRHILSVLPEWPSNRVDELLPWNVVLTDK"
     gene            443..1057
                     /gene="istB"
                     /locus_tag="KJL99_004061"
                     /pseudo
     CDS             443..1057
                     /gene="istB"
                     /locus_tag="KJL99_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522280.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc10 family helper ATPase
                     IstB"
     gene            complement(1226..5320)
                     /gene="sepA"
                     /locus_tag="KJL99_004062"
     CDS             complement(1226..5320)
                     /gene="sepA"
                     /locus_tag="KJL99_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SepA"
                     /protein_id="HBD4438594.1"
                     /translation="MNKIYYLKYCHITKSLIAVSELARRVTCKSHRRLSRRVILTSVA
                     ALSLSSAWPALSATVSAEIPYQIFRDFAENKGQFTPGTTNISIYDKQGNLVGKLDKAP
                     MADFSSATITTGSLPPGDHTLYSPQYVVTAKHVSGSDTMSFGYAKNTYTAVGTNNNSG
                     LDIKTRRLSKLVTEVAPAEVSDIGAVSGAYQAGGRFTEFYRLGGGMQYVKDKNGNRTQ
                     VYTNGGFLVGGTVSALNSYNNGQMITAQTGDIFNPANGPLANYLNMGDSGSPLFAYDS
                     LQKKWVLIGVLSSGTNYGNNWVVTTQDFLGQQPQNDFDKTIAYTSGEGVLQWKYDAAN
                     GTGTLTQGNTTWDMHGKKGNDLNAGKNLLFTGNNGEVVLQNSVNQGAGYLQFAGDYRV
                     SALNGQTWMGGGIITDKGTHVLWQVNGVAGDNLHKTGEGTLTVNGTGVNAGGLKVGDG
                     TVILNQQADADGKVQAFSSVGIASGRPTVVLSDSQQVNPDNISWGYRGGRLELNGNNL
                     TFTRLQAADYGAIITNNSEKKSTVTLDLQTLKASDINVPVNTVSIFGGRGAPGDLYYD
                     SSTKQYFILKASSYSPFFSDLNNSSVWQNVGKDRNKAIDTVKQQKIEASSQPYMYHGQ
                     LNGNMDVNIPQLSGKDVLALDGSVNLPEGSITKKSGTLIFQGHPVIHAGTTTSSSQSD
                     WETRQFTLEKLKLDAATFHLSRNGKMQGDINATNGSTVILGSSRVFTDRSDGTGNAVS
                     SVEGSATATTVGDQSDYSGNVTLENKSSLQIMERFTGGIEAYDSTVSVTSQNAVFDRV
                     GSFVNSSLTLGKGAKLTAQSGIFSTGAVDVKENASLTLTGMPSAQKQGYYSPVISTTE
                     GINLEDNASFSVKNMGYLSSDIHAGTTAATINLGDSDADAGKTDSPLFSSLMKGYNAV
                     LRGSITGAQSTVNMINALWYSDGKSEAGALKAKGSRIELGDGKHFATLQVKELSADNT
                     TFLMHTNNSRADQLNVTDKLSGSNNSVLVDFLNKPASEMSVTLITAPKGSDEKTFTAG
                     TQQIGFSNVTPVISTEKTDDATKWVLTGYQTTADAGASKAAKDFMASGYKSFLTEVNN
                     LNKRMGDLRDTQGDAGVWARIMNGTGSADGDYSDNYTHVQIGVDRKHELDGVDLFTGA
                     LLTYTDSNASSHAFSGKNKSVGGGLYASALFNSGAYFDLIGKYLHHDNQHTANFASLG
                     TKDYSSHSWYAGAEVGYRYHLTKESWVEPQIELVYGSVSGKAFSWEDRGMALSMKDKD
                     YNPLIGRTGVDVGRAFSGDDWKITARAGLGYQFDLLANGETVLQDASGEKRFEGEKDS
                     RMLMTVGMNAEIKDNMRLGLELEKSAFGKYNVDNAINANFRYVF"
BASE COUNT         1391 a         1419 c         1346 g         1498 t
ORIGIN      
        1 gccgtcaggc agcagcgaag caaagcgtta ctgacgtcgc tgaatgaatg gatggtggag
       61 aagaatggta cgctgtcgaa aaaatccaga ctgggcgaag cgttcagcta tgtactgaat
      121 cagtgggatg ccctctgtta ttacagtgat gacggtctgg cggaggcgga caataacaca
      181 gcagaaagag cgcttcgtac cgtctgtctc gggaaaaaga attacatgtt cttcggcagc
      241 gatcatggag gcgatcgcgg agctctgctg tacggactaa tcgggagctg tcggttgaac
      301 ggtatcgatc cagaagcgta tctgcgccat atcctgagcg tactgccgga atggccttcc
      361 aaccgagttg acgaactgct gccatggaac gtagttctca ccgataaata agcgtcaata
      421 cggccttccg ttgacgctta cagaactcct gtggcgcgag agtgaaaaac tgcgtcgtta
      481 taaaaaggag gcccggttgc cagttgccaa aacgctaagc gaatacgact tcattcagtt
      541 gccggaactg aatggagctc agttccagca actctgtgaa acgacagact gggttgatgc
      601 tggagaaaac gttctgctgt tcggggccag cgggttgggg aaaagccatc tggcggcagc
      661 gatcgtggat ggcgtagtag gccagggcta ccgggcccgg ttctacagcg caggagagct
      721 gttgcaggaa ctacgtaaag ccagagctca gttgaaactg aatgagctgc tactgaaact
      781 ggatcgctac cgggtgatag tggtggatga tcttggctat gtcaaacgcg acagcgccga
      841 aacgggtgtg ctgttcgagt taatagcgca tcgctatgaa cgtgggagcc tggtgataac
      901 cagtaaccat ccgttcagca tgtggggcag catcttcgtg gatgagacta tggcggtggc
      961 ggcagcagac cgactgatcc atcacggata tatgttcgaa ctgaaagggg aaagctacag
     1021 gaaaaagaca gcgaaggcag taacaagcgt gacttgatgt cgcactgaag gggcggccag
     1081 tatagttggc gccagtcggc aaaactagtt gacgtctaat acctggacgg tcacttctga
     1141 gaaaaggcat ttacacagaa tcttaaacag gctcacggag ttctttcaga aaaaggcctg
     1201 ccgaatggca gacctatccc attcgtcaga aaacataacg gaagttggcg tttatcgcat
     1261 tatccacatt atatttcccg aacgctgatt tctccagctc cagtcccaaa cgcatattat
     1321 ccttaatttc cgcattcatc cctaccgtca tcagcatcct gctatctttt tcaccttcga
     1381 aacgtttctc tccggaagca tcctgcagaa ccgtttctcc gttcgccagc aggtcgaact
     1441 gataacccag cccggctcga gctgtgattt tccagtcgtc tccggagaag gctcttccca
     1501 cgtcaacacc agtacggcca atcagtgggt tataatcctt gtctttcatg ctcagagcca
     1561 ttccccggtc ttcccagcta aaagcttttc ctgatacaga accgtaaacc agctctatct
     1621 gtggctccac ccaggactct ttcgtcaggt ggtaacgata accaacttca gcaccggcat
     1681 accaggaatg agagctgtag tcttttgttc ccagtgaggc aaaattcgcc gtgtgctgat
     1741 tatcatgatg gagatattta ccaatcaggt caaaataagc tccggaatta aagagtgcag
     1801 aggcatacag accgccaccc acggatttgt tttttccact gaatgcgtgg ctgcttgcat
     1861 tgctgtccgt ataggtcagc aatgcccccg taaataaatc cacaccgtcc agctcatgct
     1921 ttctgtcgac accaatctga acgtgagtgt agttatcgct gtagtcacca tctgccgaac
     1981 cggtaccatt cattatgcgt gcccagacac cggcatcccc ctgagtatcc cgcaggtcac
     2041 ccatacgttt gttcaggtta ttgacctctg taaggaagga cttataacct gatgccataa
     2101 agtcttttgc ggctttcgag gctccggcat cagcggtagt ctgatatcct gtcagcaccc
     2161 attttgtggc atcatccgtt ttttccgtgc tgattactgg cgtaacgtta ctgaaaccaa
     2221 tctgctgcgt accggcagtg aacgttttct catcactccc tttcggtgcg gtaattaacg
     2281 tcacgctcat ttcactggct ggtttgttta aaaagtcaac caggacgcta ttattactgc
     2341 ctgacagttt gtctgtgaca ttcaactggt cagcccggct gttgttggta tgcatcagga
     2401 acgtggtatt atctgcagaa agctctttta cttgtaaagt ggcaaagtgt ttcccgtccc
     2461 ccagttcaat tcgcgagccc ttagccttca gtgcaccggc ctctgatttt ccgtcagagt
     2521 accacagagc attgatcata tttaccgtac tctgtgcccc cgtaatactg cctctcagaa
     2581 cagcgttata tcccttcatt aaggagctga ataacggaga gtccgtcttc cctgcatcag
     2641 catcactgtc tcccagatta atggttgccg cagtggttcc tgcatgaata tccgaactca
     2701 gatagcccat atttttaaca gaaaagctgg cgttatcttc gaggttaatc ccttctgttg
     2761 tagaaattac gggtgagtaa tacccctgtt tctgcgcaga aggcatcccc gtcagggtca
     2821 gagaagcatt ttcttttaca tccacagccc cggtgctgaa aataccgctc tgagccgtaa
     2881 gttttgctcc ttttccgagg gtcaggctgc tgttgacaaa gctgccaaca cggtcaaaaa
     2941 cagcattctg agaggtcaca ctcacggtgc tatcataagc ctcaatacca ccggtgaatc
     3001 tctccatgat ttgcagggat gatttatttt ccagcgtaac atttccgctg taatcactct
     3061 ggtcaccaac tgtggttgca gtggcactcc cttcaacaga ggagaccgca ttaccggttc
     3121 cgtcactcct gtcagtaaag acacggctac ttcccagaat gactgtactt ccattggtgg
     3181 cgttaatatc tccctgcatc ttgccgtttc tggacagatg gaatgttgcc gcatcgagtt
     3241 tcagtttttc cagcgtaaac tgacgggttt cccagtcgct ctggctggaa gaagtcgtcg
     3301 ttccagcatg aataactgga tgaccctgga atatcagcgt gccggacttc ttggttatcg
     3361 aaccttctgg caggttaact gaaccatcaa gagccagtac atccttacct gagagctgcg
     3421 gaatattcac atccatattg ccgtttagtt ggccgtgata catataaggc tggctgctgg
     3481 cctcaatttt ttgttgcttc acagtatcaa tggctttatt acgatctttt ccgacattct
     3541 gccagacact gctgttgttc agatcggaga agaacgggga atatgaactc gctttcagga
     3601 tgaaatattg cttggtagaa ctgtcataat ataggtctcc cggcgctcct ctccccccaa
     3661 aaatactgac agtattaacc ggtacattga tgtcactggc ttttaatgtc tgaaggtcga
     3721 gtgttaccgt tgattttttc tcactgttat tggtaatgat ggccccgtag tcagccgcct
     3781 gaagacgggt aaaggtcaga ttattaccat tcagttccag acgtcctcca cggtatcccc
     3841 acgaaatatt atccggatta acttgctgtg agtcagaaag cacaactgtc ggacgaccgc
     3901 tggcaatacc aacagagctg aaagcctgta cctttccgtc agcatccgcc tgctggttga
     3961 gaataacggt accgtccccc accttgagtc cgcctgcatt cacaccggtc ccgtttactg
     4021 tcagggtacc ttcgccagtt ttatgcaggt tatcaccggc aactccgttc acctgccaca
     4081 gaacgtgggt tcctttatcg gtgataatcc cgccccccat ccatgtctgg ccgttcaggg
     4141 cggacaccct gtaatcgccg gcaaactgca gatacccagc tccctgatta acggaattct
     4201 gcagaacgac ctcgccatta ttgccggtga acaggaggtt ttttcctgcg ttcagatcat
     4261 ttcctttctt tccatgcata tcccaagtcg tgtttccctg agtcagtgtg ccggtgccat
     4321 tagccgcatc atatttccac tgcagtactc cttcaccaga ggtgtaggct atagttttgt
     4381 caaaatcatt ttgcggttgc tgaccaagga aatcctgagt ggtgacgacc cagttatttc
     4441 catagttagt tcctgaggaa agcacgccaa tcagtaccca ttttttttgc agggaatcat
     4501 aagcaaacag gggggagccg ctgtccccca tattcagata attagccaga ggtccattcg
     4561 cgggattaaa aatgtcaccg gtctgagctg taatcatctg tccgttgtta taggagttca
     4621 gcgcgctgac ggttccccca acaagaaatc cgccattggt atacacttgg gtgcgattac
     4681 catttttgtc tttgacgtac tgcatccccc ccccaaggcg atagaattca gtaaagcgtc
     4741 cgccggcctg ataggcaccg ctcacagccc cgatgtcaga cacttctgcc ggcgccacct
     4801 ctgtcaccag tttactcagg cggcgggttt taatatcaag accgctatta ttatttgtac
     4861 caaccgcagt ataggtgttt ttggcgtatc cgaagctcat cgtgtcagaa ccgctgacat
     4921 gtttggcggt aaccacatac tgaggggagt aaagtgtatg gtctcccgga ggcaggctac
     4981 cggtagtaat agtggcacta ctgaaatcag ccatcggggc tttatcaagt ttaccgacca
     5041 gattgccctg cttgtcgtaa atggaaatat tcgtggtccc cggcgtaaac tgacctttat
     5101 tctcggcaaa gtcgcggaat atctgataag ggatctctgc actgaccgtt gctgacagag
     5161 ctggccatgc cgaggaaagt gataaagctg caacagacgt aagaataacc cgacgtgaaa
     5221 gtctgcgatg gcttttgcag gtaaccctgc gggccagttc ggagacagca atcaggcttt
     5281 tggttatatg gcaatactta agataatata ttttgttcat atagaaacat caacctaatt
     5341 ttgacaaaga ttaattacat aggatgtaga tggagcagtc cgagattcta ctaatatcaa
     5401 ttattaagca tctgtttttt agttggtgta atataatttt tattaaggta agcggaaaaa
     5461 caaccaaaaa aaacgcaaga tgttattttt ttatcatttt gagccagttt ggctgggggg
     5521 atactcttct cttaagcttc ttgctcaact ttaaaaatag ttatgccagt tcggtatcga
     5581 taaggggcag tttggatatc gagaaccacg gcagcctgac ggctgcaaat tgaaaactgc
     5641 ccctgttcgt cctg
//
LOCUS       DAEBXN010000190         5631 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.191, whole genome shotgun sequence.
ACCESSION   DAEBXN010000190 DAEBXN010000000
VERSION     DAEBXN010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5631
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.191"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..706
                     /locus_tag="KJL99_004063"
     CDS             <1..706
                     /locus_tag="KJL99_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBD4438595.1"
                     /translation="KKLPLNSKAILAYATGVFEPFLAYTFTLYGLYFISAGSASIIFS
                     LESVFILVLSVLICSVKINSPQYFILFLIGAIVGSILVALPDISNGSNNIVGYCLVVA
                     GVLSAAFYVVISSRLIKSFEPITLLSGQLTFSLILSSLFIFISKSSIYLPLESVIIVF
                     SSGILQYFLAFCFYLNSLRWLPVHLAGIMLYFIPLVALFLSWLFLDEQFTFLQGVGFI
                     VTVLSVYFLNRKYSEE"
     gene            complement(1909..2100)
                     /locus_tag="KJL99_004064"
     CDS             complement(1909..2100)
                     /locus_tag="KJL99_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HBD4438596.1"
                     /translation="MTKQEKTALNMARFIRGQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDGENR"
     gene            2165..2488
                     /locus_tag="KJL99_004065"
     CDS             2165..2488
                     /locus_tag="KJL99_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HBD4438597.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPT
                     LAPPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQG
                     ARDDS"
     gene            2430..3188
                     /locus_tag="KJL99_004066"
                     /pseudo
     CDS             2430..3188
                     /locus_tag="KJL99_004066"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3166..3696
                     /locus_tag="KJL99_004067"
     CDS             3166..3696
                     /locus_tag="KJL99_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HBD4438598.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVNRELTSN
                     ESLIRNAINDHTTALKELLERYQKTTVDTMDAHWKTVLKMSVKRWLWLIIVSVLMFAT
                     TGSLLWYQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPKGMKAETN
                     WTKDNGKLNAVRLVQE"
     gene            3703..3936
                     /locus_tag="KJL99_004068"
     CDS             3703..3936
                     /locus_tag="KJL99_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HBD4438599.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWENAFVELQKMFSLTQRDN
                     AMLNGRVMQLSQQVQHLSERTERLSQLYSENRR"
     gene            4417..5469
                     /locus_tag="KJL99_004069"
     CDS             4417..5469
                     /locus_tag="KJL99_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438600.1"
                     /translation="MNLQQRINKLPQLSSSFSFGKDIDNIHSFIFNETSKDKIEDLLR
                     KWVSGNQPCVFGKLASKKIKGLDFHLSIVNSPQLYNDDGHLFDFLRNERVRFKERARR
                     GEVSAHLIYFIHPQLAFARPSEELVDIQKYICSLHMPECYPIKEDVIYTESVPFQDKD
                     GLKIYKAGVNVFYSSAHRTRNHDRRIPGGILISVNAPGHFMRLAIEKGFYKDQEQALA
                     DIRNMTIQSVGKGGYSHPEGISTTWHSESNLDRFGCPVHTGNSSYYSGFYHTDVLIPG
                     ELTKDERLLHEIENSDPMIFNWNVLFYVSLEEFPIDDPYYGEFIGIPVDDASMFFNSF
                     QPRKFENKPLYEKEDD"
     gene            5471..>5631
                     /locus_tag="KJL99_004070"
     CDS             5471..>5631
                     /locus_tag="KJL99_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBD4438601.1"
                     /translation="MIKGMLFAFLAAFSWGAAIVMSKKGVEELDAGGVFFWQICSAVI
                     LSWIVLFIK"
BASE COUNT         1486 a         1154 c         1514 g         1477 t
ORIGIN      
        1 aaaaaaactc ccattaaact caaaagcgat acttgcttat gcgacaggcg tttttgaacc
       61 attcttagcg tatactttta cactctacgg actttatttt atttcagcag gaagcgcatc
      121 aattattttt tcacttgaat cagtgtttat attggtgttg tctgttctta tttgctcagt
      181 taaaattaat tccccgcaat attttatatt gttccttata ggtgcaattg ttggcagtat
      241 tttggtagcc ttgccagaca tcagtaatgg atcgaataat attgttggat attgtcttgt
      301 tgttgccggt gtgttatcgg cagcatttta tgttgttata tcgtctcgcc ttattaaatc
      361 gtttgagcct atcacattat tgagcggaca gttgacattc tcccttatac tgtcatcgct
      421 ctttattttt atatctaaat catcaattta tcttccgttg gaatccgtga ttattgtttt
      481 ttcatctgga atattacaat attttttagc tttttgtttt tacttgaact ccttgcgttg
      541 gctcccagtt catttggcag ggataatgtt gtactttatt ccgctagttg cattattttt
      601 gtcatggttg tttttggatg aacaattcac attcttacag ggggtaggat ttattgtaac
      661 tgtgctaagt gtatattttc ttaacagaaa atactcagag gagtaacatt ctgttcagga
      721 ttgccccccg gcctccatcg ataccgtgcc aaatgcacgt aaaaaacggg agcgtttctg
      781 gtgtcggaat gtaaccaagg gggtcagagg cgcgctaaaa cgctctcagg ggagtcaaat
      841 agagacgttt gtaacaaaaa gatcaaagga tcctcttgag atcctttttt tctgcgcgta
      901 atctgctgcc tgtaaacgaa aaaaccgcct tgcagggcgg tttgatcgaa ggttaagtca
      961 gcttgcagac tgcttaacca ggtaactgtc ttatgcgaga cgagtcacca aaattgttct
     1021 tctagtgtag ccgcactccg gcagccactt caagtactct ctaaatatct cgcacatgac
     1081 ttttaccaat ggctgctgcc agtggcgctt tgtggtgtct tttccgggtt ggacccaaga
     1141 gaacagttac cggagaaggc gcagcagtcg ggctgaacgg ggggttcctg catacagccc
     1201 agcccggagc gaactgccta agtcgccccg agataccaga agacgtgagc tatgagaaag
     1261 cgccacgtgt cccgtaaggg agaaaggcgg aaaaggtatc cggtaatcga tatggcggga
     1321 acgtgggggc gcaggaggga gcgattcacc ggaaacggtg atgatcttta agccacgtcg
     1381 ggtttcgcca ccgctggcgg atttcatggt tgagccgcaa ggctcccaca gatgcaccga
     1441 aaaagtgtct gttttgtgaa gtccgtcagg ggggcggagc ctatggaaaa agcgcctccg
     1501 gcgcggcctt tcccggggtt tcgttttttg cgcacatgtt ctttcctggt ttatccccgg
     1561 gttctgtgga taacagggtt agcgtttttg agaaaggaga gtccgctcgc cgcagccgaa
     1621 cgaccgagcg tagcgagtca gtgagcgagg aagcggaaga gcgcctgaac tggcattttc
     1681 tccttacgca tctgtgcggc atttcacacc ggacatggcg cacttttcat acaatccgca
     1741 ctgatgccga atggttaagc cagtatacac tccgctatcg ctacgtgact gggtcagggc
     1801 ttcgccccga caccccaaag gtcaacggcc tgacgcgccc tggcgggctt gtctgctccc
     1861 ggcatccgct cacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agcggttttc
     1921 accgtcatcc ccgaagcgtg cgaggcaact gcggtaaagc tcatcggcgt ggtcgtgaag
     1981 cgattcacag atgtctgcct gttcatccgc gtccagttcg ttgagcttct ccagcagcgt
     2041 taatgtctgg cctctgatga agcgggccat gttaagggcg gttttttcct gtttggtcac
     2101 tgatgcctcc gtgtaagggg gggttctgtt catgggggta atgataccga tgaaacgcga
     2161 gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgctgtga
     2221 gggtaaacaa ctggcggtat ggatgcggcg ggtctgcctg ggtgagccgg ttgcccgttc
     2281 cgggaagctg ccgacactgg caccgccgtt actgcgtcag ctggccgcca tcgggaataa
     2341 ccttaaccag acagcgagga aggtgaacag cgggcagtgg tcttccggtg accgggttca
     2401 ggtggtggcc gcactgatgg ccatcgggga tgagctgcgc cggctgcgtc tggcggtcag
     2461 ggaacagggg gcgcgggatg atagttaagt ttcatgccag gggaaaaggt ggtggcagtg
     2521 gtccggttga ttacctgctg ggaagggagc gtaaccggga aggggcaacg gtgcttcagg
     2581 ggaatccgga agaggtccgg gaactcatcg atgccacgcc atttgcgaag aaatacacgt
     2641 caggtgttct gtcgttcgcg gagaaggagc tgccgcctgg agggcgtgaa aaagtgatgg
     2701 cgagctttga gcgtgtactg atgcccggtc tcgagaaaaa tcagtacagc atcctgtggg
     2761 tggagcacca ggacaaggga cggcttgagc tgaattttgt cattccgaac atggagctac
     2821 agaccggaaa acgcctccag ccgtactacg accgggcaga caggccgaga attgatgcct
     2881 ggcagacgct ggtgaatcac cattacggtc tgcatgaccc caacgccccg gagaaccgcc
     2941 ggacgctgac ccttcctgat aacctgccgg aaacgaaaca ggcgcttgct gagggcgtca
     3001 cacgcgggat agacgcgctt taccatgccg gagagataaa aggccgtcag gatgtgattc
     3061 aggcgctcac agaggcgggg cttgaagtgg tcagggtgac gcgaagcagt atcagcattg
     3121 cagacccgaa cggcgggagg aatatcagac tgaaaggagc gttttatgag caatctttta
     3181 cagacgggcg cggagttcga gaaaaagctg aaagagagag ccgaatctac agagaaaatg
     3241 ctgaacgacg agttcaggaa gctcgaagaa tctgtaaaca gggaactgac ctcaaacgaa
     3301 tctttaatca ggaacgctat caacgaccat acaacagccc tgaaagaact gctggagagg
     3361 tatcagaaaa cgaccgtcga tacgatggac gcgcactgga aaaccgtact gaaaatgagc
     3421 gtgaaacgct ggctgtggct gattatcgtc tcagtactga tgtttgccac gaccgggagt
     3481 ctgctgtggt accagggcat gaagataaac gcgaatatga atattctccg cgagcagaaa
     3541 gagagtctgg agaagctgaa cgcgaagaca tggggcgtgc gttatcacga ggacagcaac
     3601 ggacgttttc tggtgttgcc gaaggggatg aaagccgaaa cgaactggac gaaggacaac
     3661 ggcaaactga acgcagtgag actggtgcag gagtgacaga gcatgacgga gctggaaaga
     3721 catttgctga acgcattaga gcaactgcaa caggactata tgcagcggct gaacgaatgg
     3781 gagaacgcct tcgtggaatt gcagaaaatg ttttcgctta cgcaacggga caacgcgatg
     3841 ctgaacgggc gagtcatgca gttgagtcag caggtgcagc acttgagcga gcggacagaa
     3901 cgcttgagcc agttatacag cgagaacagg agataaggga tgaacggctg atgcaggaac
     3961 gcgaacatga gttatctctg gagcgggagc gtcagccgga aatccaggag cggacactgg
     4021 atggcccttc gctgggatgg tgaaaccatg aaaaatgacg tgttcagtgg attaaggcgg
     4081 gctaatgtgg cctgtagcat ctggttgcat aggtattcat gcggttaaaa tttatcaggt
     4141 gcgatcgcgg cagtttttcg ggtggtttgt tgccattttt acctgtctgc tgccgtgatc
     4201 gcaccgaacg cgttttggcg gtgcgtacaa tgcgacgtta tggtaaatgg agcggatttt
     4261 caggaaggag tgatactaac aactcgaaca ctgtgcaaat aatgattttt ttaaaatatc
     4321 ggtaacgata ataaattatt tcgttttacg ccggtaataa atataatgta gtttcagtat
     4381 ataaaaaaat atatgaggtg tcttgtggga gctttcatga atcttcaaca acgtataaat
     4441 aaactaccac aattatcatc cagtttttca tttggaaaag atattgataa tattcattca
     4501 tttatcttta acgaaacttc taaagataaa attgaagatt tactaagaaa atgggtgtca
     4561 ggcaatcagc cctgtgtatt tggtaaattg gcaagcaaga aaattaaagg gcttgatttc
     4621 catttatcta ttgtaaatag cccccaacta tataatgatg atggtcattt gtttgatttt
     4681 ttgcgtaatg aaagagttcg ttttaaagaa cgcgcaagaa gaggagaggt cagtgcgcat
     4741 ctcatttatt ttattcatcc acaacttgcc tttgctcgtc catcagaaga gttagtggac
     4801 atacaaaaat atatttgttc gctgcatatg ccagagtgct atccaataaa agaggatgtt
     4861 atttatactg aaagcgtacc atttcaggat aaagatgggc taaaaatata taaggctggt
     4921 gttaatgttt tttactcttc tgctcataga acaagaaatc atgatagaag aataccaggg
     4981 ggaatactta tttctgtaaa tgccccgggg cattttatga ggctggcgat agaaaaaggt
     5041 ttctataaag atcaagaaca agcgttagcg gatattcgta acatgactat tcagtctgtt
     5101 ggtaagggtg ggtattcaca tccggaaggg atttctacaa catggcattc agaatctaat
     5161 ttggatcgtt ttggatgccc agtgcacacc ggtaatagtt cttattacag cggtttttat
     5221 catactgatg tattaattcc aggagaatta actaaggatg aaagattgct ccatgaaata
     5281 gaaaatagtg atccaatgat cttcaactgg aatgtattgt tttatgtttc attagaagag
     5341 tttcctatag atgatcctta ttatggagag tttattggta ttcctgtaga tgatgcatct
     5401 atgtttttta actcctttca accaaggaaa tttgaaaaca aaccccttta tgagaaagag
     5461 gatgattaat atgattaaag gaatgctttt tgctttttta gcagcgttct catggggggc
     5521 agctatcgta atgagcaaaa aaggagttga agaactagat gccggagggg tgtttttctg
     5581 gcagatttgt agtgcagtaa tactatcttg gatagttctt tttataaaaa g
//
LOCUS       DAEBXN010000191         5592 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.192, whole genome shotgun sequence.
ACCESSION   DAEBXN010000191 DAEBXN010000000
VERSION     DAEBXN010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5592
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.192"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(1..279)
                     /locus_tag="KJL99_004071"
     CDS             complement(1..279)
                     /locus_tag="KJL99_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438602.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDGYQHDPCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPSCTFQQRIWR"
     gene            220..405
                     /locus_tag="KJL99_004072"
     CDS             220..405
                     /locus_tag="KJL99_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438603.1"
                     /translation="MPAGRGGRVIRIVKFGIVRHLFSLLAQQVPVVQADGWIVNQNDQ
                     KKQNPPKRVKCGCVEDA"
     gene            complement(447..602)
                     /gene="hokD"
                     /locus_tag="KJL99_004073"
     CDS             complement(447..602)
                     /gene="hokD"
                     /locus_tag="KJL99_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HBD4438604.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(1254..1670)
                     /locus_tag="KJL99_004074"
     CDS             complement(1254..1670)
                     /locus_tag="KJL99_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438605.1"
                     /translation="MTELTKEQLIEEAKLKIAITKCHPNSGMARVEGELFKIARASLE
                     AEPIAWRYRYVKKGVMDSQGELWVGDWKYVPKKEDCNDRPNYEIQALFTAPPVPVTSE
                     ELVKAVHFYEQLKRENPPASGNQINGLTMSVKRPAN"
     gene            1697..1837
                     /locus_tag="KJL99_004075"
                     /pseudo
     CDS             1697..1837
                     /locus_tag="KJL99_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
     gene            1837..2862
                     /locus_tag="KJL99_004076"
     CDS             1837..2862
                     /locus_tag="KJL99_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBD4438606.1"
                     /translation="MGRRRKNPEHEKLPPNVYPNKYSYVWKPTSRESVTLTAIKDGLA
                     ALWKKYEETVNNRDRAMTFGRLWEKFLASAYYSDLSPRTQKDYLQHQKKLLAVFGKVP
                     ADSIKPEHIRRYMDKRGEQSKTQANHEKSSMSRVYSWGYERGYVKANPCAGVSKFKAK
                     NRERYVTDKEYQAVLSVAPLPVFIAMEIAYLCAARVSDVLSLKWEQIGNDGIFIQQGK
                     TGKKQIKAWSPRLQAAIEKAKQLPTSAYVISNQYGNRYMYKGFNEMWVEARNRAGKIS
                     GILTDFTFHDLKAKGISDYEGSSRDKQLFSGHKTEGQVLIYDRKVKVSPTLDVPLPEN
                     IPRKYSK"
     gene            complement(2915..3004)
                     /locus_tag="KJL99_004077"
     tRNA            complement(2915..3004)
                     /locus_tag="KJL99_004077"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2968..2970),aa:Ser,seq:cga)
     gene            3098..3895
                     /gene="mtfA"
                     /locus_tag="KJL99_004078"
     CDS             3098..3895
                     /gene="mtfA"
                     /locus_tag="KJL99_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HBD4438607.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            3996..4071
                     /locus_tag="KJL99_004079"
     tRNA            3996..4071
                     /locus_tag="KJL99_004079"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4029..4031,aa:Asn,seq:gtt)
     gene            4233..5495
                     /locus_tag="KJL99_004080"
                     /pseudo
     CDS             4233..5495
                     /locus_tag="KJL99_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         1509 a         1326 c         1293 g         1464 t
ORIGIN      
        1 ttacctccag atccgttgct ggaatgtgca ggacggacgc ggtggccgtt cggaataagg
       61 gagcctgacg aagattatcc agtgacgata atcgagactg agggctttct taatctcgta
      121 tccgtgtctg cggtagcgct gaatcagcca ttcagcctgt tcttcggtgc aggggtcgtg
      181 ctgatacccg tcagatttga atgcatgaga acgccgcccg tgcctgctgg caggggcggc
      241 agagttatcc gaattgtaaa atttggtatc gtgcgccatc tgttttctct gctggcgcag
      301 caggtgccag ttgttcaggc tgacggatgg attgtaaacc agaacgacca gaaaaaacaa
      361 aacccgccga agcgggttaa gtgcgggtgc gttgaggatg cctgacacat cagcggtggc
      421 gagggatttc tcccccgccg ggtctcttac tcctcaggtt cgtaagctgt gaagacagcg
      481 acctccgtct ggccggttcg gattcgtacc tcgcagaggt ctttcctcgt taccagtgcc
      541 gtcactatga cggttaaaca gatgacgata agggcgatta acatcgcctt ttgctgcttc
      601 atagcctgct tctccttgcc tttcggcacg taagaggcta acctacatgt gttcagcatg
      661 gattgagcct cagattaatg ttaagcgtct tgcaggacgc gtaatgttaa ctggggcttt
      721 tctctatctg ccttttggtg ttcatgcctg agacagatag cctcaagcac ccgcagtcat
      781 tctacttaac taagatttcc ctgcaaaccg tttttgtccg gcacagtaaa tatccaacta
      841 aaccaatagc gttcgctgta tttaccgcca gtattcaatg cacatgaccg ccatgaacac
      901 ccctaaaaaa agggcattta tatgtccaaa cattaatatc aaaacatcaa ttttttccat
      961 ataccttgct gtgaagatga tgggcataca tgatgcgaac aaccagaacg caacaaacaa
     1021 aaactgcaat gcgtttttca ttattccccc tacaatcaat gtgcaataac atttaaacac
     1081 acctcaattt ggccggacat ataaatatct aaaccagaaa aaatcactta catagcgtta
     1141 caaactcttt agtctaaata ctcatcgtaa aacattcccc atacttatca gtccgttccg
     1201 cgccaggtag cttattgcct tatctggcaa cctataatca ggtttccgct ttttcagttg
     1261 gctggtcgtt taaccgacat agttaaccca ttaatctggt tgccggatgc tggtggattt
     1321 tcgcgtttta attgttcata aaagtgcaca gctttaacca gttcttctga tgtaaccggg
     1381 actggtgggg cagtgaataa ggcctgaatt tcatagttcg gcctgtcgtt acaatcctct
     1441 tttttcggta catatttcca gtcaccaacc cacaactccc cctgagagtc cataacacct
     1501 tttttcacgt agcgatatcg ccacgctatc ggctctgctt ccagcgatgc ccgtgcaatt
     1561 ttgaataact cgccctctac tcgcgccatc cctgaattgg ggtggcattt cgtaatcgct
     1621 atttttaatt tggcttcttc gattaattgt tcttttgtta attcagtcat tttcattacc
     1681 gccctggtaa tgctgccacc agcgaaaatg gttaatggaa aacgggcttc cgttctatac
     1741 caatcgtagt ggcaaaccga ttgtcagccg ggatctattt acctgcaata aaactttacc
     1801 accacgcgag gtagagccga attttggtgc gatctgatgg gaagacgaag gaaaaatcct
     1861 gaacacgaaa aattacctcc aaatgtatac ccaaataaat atagttatgt atggaaacca
     1921 acatccagag aatctgtaac actaaccgcc atcaaggatg gtttagctgc tttatggaaa
     1981 aagtatgagg aaactgtaaa taatcgcgat cgtgcaatga cattcggtcg cttgtgggaa
     2041 aaattcctcg ccagcgccta ttacagtgac ctcagtccaa gaacacaaaa agattatctg
     2101 caacatcaaa aaaagttgct tgccgtattc ggtaaggtgc cggcagattc cataaaacca
     2161 gaacacatcc gtcgatacat ggacaagaga ggggaacaga gtaaaacgca agccaaccat
     2221 gaaaaaagca gtatgtcccg cgtttacagt tgggggtatg agcgagggta cgtgaaggct
     2281 aacccatgtg caggtgtaag taaattcaag gccaaaaacc gcgaacgata tgtaaccgac
     2341 aaagaatacc aggcagtatt aagcgttgca cctcttcctg tttttatcgc aatggaaatt
     2401 gcctatctgt gtgcagcgag ggtttccgat gtgttatcgc tgaaatggga gcagattgga
     2461 aacgacggga tctttatcca gcaagggaaa acaggaaaaa aacagataaa agcatggagt
     2521 ccacgattac aggcggcgat cgaaaaagca aaacagttac caacatccgc ctatgtaatc
     2581 agcaatcaat acggcaaccg atatatgtac aaaggcttta acgaaatgtg ggtagaagca
     2641 agaaatcgcg caggcaaaat ttcaggtatt ttaaccgact tcacctttca tgatctgaag
     2701 gcgaaaggaa tttcagacta tgaaggaagc agtcgggata agcaactttt ctctggtcac
     2761 aaaaccgagg ggcaagtgct aatctatgac aggaaggtta aagtttcacc gacacttgat
     2821 gtcccgttac ctgaaaatat tccaagaaaa tattccaagt aattccaagt gtgatttttg
     2881 tcactgactt aatgatgtat aagtgattga attttggcgg agagaggggg atttgaaccc
     2941 ccggtggagt tgcccccact ccggttttcg agaccggtcc gttcagccgc tccggcatct
     3001 ctccgttcag atggttgcca tgatgccagg aaatttggca ttttaacagt ccctgtccgt
     3061 gcaattttgt tcaagtgacg agtttgcgag caaaacgatg attaagtggc cctggaaagt
     3121 acaagaatca gcacatcaaa ctgcccttcc ctggcaggaa gcactatcga tccccctttt
     3181 aacgtgtctg acagaacagg aacaaagcaa attggtcgct cttgccgaac gttttttaca
     3241 gcaaaaacgg cttgttcctt tacagggctt tgagctgaat tcattaagaa gctgccggat
     3301 agcacttcta ttttgcctgc ccgttctgga gttaggactg gaatggctgg atggttttca
     3361 tgaagtctta atttatcctg cgccatttgt ggtcgatgat gaatgggaag acgatatcgg
     3421 tctggtgcat aaccaacgta ttgttcagtc aggtcagagc tggcagcaag ggcctatcgt
     3481 tttgaactgg ttggatatac aagattcttt tgatgcttct ggttttaacc tgattattca
     3541 tgaagtcgct cataagctgg acacccgtaa cggcgatcgc gccagcggag ttccctttat
     3601 ttcgttgcgt gaggttgctg gctgggaaca cgatcttcat gctgcaatga acaacattca
     3661 ggaagaaatc gaattagttg gtgagaatgc ggcgagcatt gatgcttatg ctgccagtga
     3721 tcctgctgaa tgttttgccg tactttctga atatttcttt agcgccccag aactttttgc
     3781 tcctcgtttc ccttcattgt ggcaacgttt ctgtcaattt tatcaacaag atcctttgca
     3841 gagactgcat cacgctaatg atacagactc gttttcagcg acgaatgttc attaattaac
     3901 aactttgaag attaattaac caattgaaat ggcttatgaa atttagtgtt gacagacaag
     3961 gtaccgctaa gtaatatgcg ccccgttctc acgattcctc tgtagttcag tcggtagaac
     4021 ggcggactgt taatccgtat gtcactggtt cgagtccagt cagaggagcc aattttctgt
     4081 tttcatgcat ccttgcgaat ctttatctga tgttgattca acaggttagt gtgaaaactc
     4141 ttctcggtgc attttgattt taccctacgc atcgggaaaa attggtggtc aaatctggga
     4201 tcaggttagt tcgataatgg agtgaccccc atatatccct taccgacgca aaaatccgca
     4261 ccctcaagcc ttctgataaa ccctttaaag tctccgattc tcacggtctg tatctgctgg
     4321 tcaagccggg tggctcccgc cactggtatc tcaaataccg tattagcggt aaagaatccc
     4381 gcattgcgct gggtgcctat ccagccatct ccctgtctga tgcgcgacag caacgtgaag
     4441 gtatccgtaa aatgctggcg ctgaatatca acccggtaca gcagcgggct gctgaacgtg
     4501 gctcacgaac accggagaaa gtttttaaaa acatggcgct ggcgtgacat aaaagtaaca
     4561 ggaaatggtc gcagaacacc gccgaccgtc tgcttgccag cctgaacaat cacatctttc
     4621 cggtcatcgg gaacctacct gtatcagaac ttaaaccccg tcatttcatt gacctgctga
     4681 aagggatcga ggaaaaaggt ctgctggagg ttgcgtcccg cacacggcag cacctgagta
     4741 acataatgcg ccatgcggtc catcaggagt taatcgatac gaaccctgca gcaaaccttg
     4801 gcggcgtgac cacacctcct gtcagacggc actatcctgc cctgccgctg gagcggctgc
     4861 ctgaactgct tgaacgtatt ggggcatatc atcagggccg tgaactgacc cggcatgccg
     4921 ttctgctgat gctgcatgtg ttcattcgct ccagtgaact gcgtttcgcc cgctggtcag
     4981 agattgattt cacaaaccga gtctggacga tacccgcgac gcgagaaccc attattggcg
     5041 tgcgttattc cggccgcggg gcaaaaatgc gaatgccgca tatcgtcccc ctctcagaac
     5101 agtccatcgc cattctgaaa cagattaagg atatcaccgg taataatgaa ctgatcttcc
     5161 ccggcgacca taacccgtat aagccaatgt gtgaaaacac ggtcaataag gcactgcggg
     5221 tgatgggtta cgacacgaaa aaggatatct gcggtcacgg cttccgggca atggcatgca
     5281 gtgcgctgat ggaatcgggt ttatgggcaa aggacgcagt agaacgccag atgagtcatc
     5341 aggagcgcaa taccgtgcgc atggcttata ttcataaggc agagcacctg gaagcccgca
     5401 aagcgatgat gcagtggtgg tcggattatc tggaagcatg ccgagaatct tatgcaccgc
     5461 cttatacaat tggtaaaaat aagtttatcc catagtatac taacgaatcc cacactctgg
     5521 caaattacca ccacctctac aggaggtggt tctatataga cttttatcaa cttcatgacg
     5581 aaaaacacct at
//
LOCUS       DAEBXN010000192         5581 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.193, whole genome shotgun sequence.
ACCESSION   DAEBXN010000192 DAEBXN010000000
VERSION     DAEBXN010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5581
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.193"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            27..242
                     /locus_tag="KJL99_004081"
     CDS             27..242
                     /locus_tag="KJL99_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001384468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HBD4438608.1"
                     /translation="MSIERFQSLATEGKMLSLSWWENEYAVLQWKNHVLHAKAQQEGR
                     ESIFDFYKISIAHITREYSFKKDKDNV"
     gene            235..621
                     /locus_tag="KJL99_004082"
     CDS             235..621
                     /locus_tag="KJL99_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HBD4438609.1"
                     /translation="MFDVHVVLDNQIGQLALLGKTLGNKGIGLEGGGIFTVGDECHAH
                     FLVEQGKEAKIALEQAGLLVLAIRTPLIRKLKQEKPGELGEIARVLAENNINILVQYS
                     DHANQLILITDNDSMAASVTLPWAIK"
     gene            629..1315
                     /locus_tag="KJL99_004083"
     CDS             629..1315
                     /locus_tag="KJL99_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBD4438610.1"
                     /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATE
                     LSSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHG
                     VHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMG
                     IDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKG
                     YAAFKTHFHI"
     gene            complement(1293..1916)
                     /gene="tetR(B)"
                     /locus_tag="KJL99_004084"
     CDS             complement(1293..1916)
                     /gene="tetR(B)"
                     /locus_tag="KJL99_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HBD4438611.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            1998..3203
                     /gene="tet(B)"
                     /locus_tag="KJL99_004085"
     CDS             1998..3203
                     /gene="tet(B)"
                     /locus_tag="KJL99_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HBD4438612.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(3316..3909)
                     /gene="tetC"
                     /locus_tag="KJL99_004086"
     CDS             complement(3316..3909)
                     /gene="tetC"
                     /locus_tag="KJL99_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HBD4438613.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            4042..4413
                     /locus_tag="KJL99_004087"
     CDS             4042..4413
                     /locus_tag="KJL99_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBD4438614.1"
                     /translation="MQLRYDDKQFHTTVIKDVLLWIEHNLDQSLLLDDVANKAGYTKW
                     YFQRLFKKVTGVTLASYIRARRLTKAAVELRLTKKTILEIALKYQFDSQQSFTRRFKY
                     IFKVTPSYYRRNKLWELEAMH"
     gene            complement(4423..>5581)
                     /locus_tag="KJL99_004088"
     CDS             complement(4423..>5581)
                     /locus_tag="KJL99_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
                     /protein_id="HBD4438615.1"
                     /translation="LHLKRLNSLTLACHALLDCKTLTLTELGRNLPTKARTKHNIKRI
                     DRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIREQKRLMVLRASVALHGR
                     SVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIVSDAGFKVPWYKSVEKLGW
                     YWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYKRLTKSNPISCQILLYKSR
                     SKGRKNQRSTRTHCHHPSPKIYSASAKEPWVLATNLPVEIRTPKQLVNIYSKRMQIEE
                     TFRDLKSPAYGLGLRHSRTSSSERFDIMLLIALMLQLTCWLAGVHAQKQGWDKHFQAN
                     TVRNRNVLSTVRLGMEVLRHSGYTITREDLLVAATLLAQNLFTHGYALGKL"
BASE COUNT         1542 a         1029 c         1215 g         1795 t
ORIGIN      
        1 cactattaag tcatgtagct ggttttattt caattgaacg ttttcaaagt ctagctacag
       61 aaggaaaaat gttatcgcta tcttggtggg aaaacgaata cgcagttctg caatggaaaa
      121 atcatgtttt acatgcgaaa gctcaacaag aagggcgaga gtcaatattt gatttttaca
      181 aaattagtat tgctcatatt actcgcgaat attcatttaa aaaggacaag gataatgttt
      241 gatgttcacg ttgttttaga taatcaaata ggacaattag cattactagg aaaaacatta
      301 ggtaataaag gtattggatt ggaaggggga gggatattta cggttggtga tgaatgccat
      361 gctcattttc ttgttgaaca aggaaaggaa gctaaaatag cgctagagca agctggactg
      421 ttagtacttg cgatccggac accattaatt cgtaagttaa aacaggaaaa accgggggaa
      481 cttggcgaaa tagcacgagt attggcggag aataacatta atattttagt gcaatacagt
      541 gaccatgcta accaactgat attaataacg gacaatgata gtatggctgc atctgttacg
      601 ctcccttggg caataaagtg aacttgcgat ggctaattta atacgaaaag aggttacctt
      661 tgagtcctca atagccgcga taggggcggc tatgtctgac atttcacgag ttaaaatact
      721 cagtgctttg atggatgggc gagcttggac ggccactgag ctaagttctg tggcgaatat
      781 atcagcttca acggcgagca gtcatttatc taaattatta gattgccagc taatcacagt
      841 agtagctcaa ggcaagcatc gttattttcg gctagcagga aaagatattg ctgaattgat
      901 ggaaagtatg atggggatct ccttaaacca tggcgtacat gccaaagttt ccacgccagt
      961 gcatttacga aaagcacgta cttgctatga tcatttagct ggcgaagttg ccgttaagat
     1021 ctatgattcc ctttgtcaac agcaatggat cactgaaaat ggttcaatga tcacattaag
     1081 tggtattcaa tattttcatg aaatgggaat tgacgttcct tccaaacatt cacgtaaaat
     1141 ctgttgtgcg tgtttagatt ggagtgaacg ccgtttccat ttaggtgggt acgttggagc
     1201 cgcattattt tcgctttatg aatctaaagg gtggttaact cgacatcttg gttaccgtga
     1261 agttaccatc acggaaaaag gttatgctgc ttttaagacc cactttcaca tttaagttgt
     1321 ttttctaatc cgcatatgat caattcaagg ccgaataaga aggctggctc tgcaccttgg
     1381 tgatcaaata attcgatagc ttgtcgtaat aatggcggca tactatcagt agtaggtgtt
     1441 tccctttctt ctttagcgac ttgatgctct tgatcttcca atacgcaacc taaagtaaaa
     1501 tgccccacag cgctgagtgc atataatgca ttctctagtg aaaaaccttg ttggcataaa
     1561 aaggctaatt gattttcgag agtttcatac tgtttttctg taggccgtgt acctaaatgt
     1621 acttttgctc catcgcgatg acttagtaaa gcacatctaa aacttttagc gttattacgt
     1681 aaaaaatctt gccagctttc cccttctaaa gggcaaaagt gagtatggtg cctatctaac
     1741 atctcaatgg ctaaggcgtc gagcaaagcc cgcttatttt ttacatgcca atacaatgta
     1801 ggctgctcta cacctagctt ctgggcgagt ttacgggttg ttaaaccttc gattccgacc
     1861 tcattaagca gctctaatgc gctgttaatc actttacttt tatctaatct agacatcatt
     1921 aattcctaat ttttgttgac actctatcat tgatagagtt attttaccac tccctatcag
     1981 tgatagagaa aagtgaaatg aatagttcga caaagatcgc attggtaatt acgttactcg
     2041 atgccatggg gattggcctt atcatgccag tcttgccaac gttattacgt gaatttattg
     2101 cttcggaaga tatcgctaac cactttggcg tattgcttgc actttatgcg ttaatgcagg
     2161 ttatctttgc tccttggctt ggaaaaatgt ctgaccgatt tggtcggcgc ccagtgctgt
     2221 tgttgtcatt aataggcgca tcgctggatt acttattgct ggctttttca agtgcgcttt
     2281 ggatgctgta tttaggccgt ttgctttcag ggatcacagg agctactggg gctgtcgcgg
     2341 catcggtcat tgccgatacc acctcagctt ctcaacgcgt gaagtggttc ggttggttag
     2401 gggcaagttt tgggcttggt ttaatagcgg ggcctattat tggtggtttt gcaggagaga
     2461 tttcaccgca tagtcccttt tttatcgctg cgttgctaaa tattgtcgct ttccttgtgg
     2521 ttatgttttg gttccgtgaa accaaaaata cacgtgataa tacagatacc gaagtagggg
     2581 ttgagacgca atcgaattcg gtatacatca ctttatttaa aacgatgccc attttgttga
     2641 ttatttattt ttcagcgcaa ttgataggcc aaattcccgc aacggtgtgg gtgctattta
     2701 ccgaaaatcg ttttggatgg aatagcatga tggttggctt ttcattagcg ggtcttggtc
     2761 ttttacactc agtattccaa gcctttgtgg caggaagaat agccactaaa tggggcgaaa
     2821 aaacggcagt actgctcgga tttattgcag atagtagtgc atttgccttt ttagcgttta
     2881 tatctgaagg ttggttagtt ttccctgttt taattttatt ggctggtggt gggatcgctt
     2941 tacctgcatt acagggagtg atgtctatcc aaacaaagag tcatcagcaa ggtgctttac
     3001 agggattatt ggtgagcctt accaatgcaa ccggtgttat tggcccatta ctgtttgctg
     3061 ttatttataa tcattcacta ccaatttggg atggctggat ttggattatt ggtttagcgt
     3121 tttactgtat tattatcctg ctatcgatga ccttcatgtt aacccctcaa gctcagggga
     3181 gtaaacagga gacaagtgct tagttatttc gtcaccaaat gatgttattc cgcgaaatat
     3241 aatgaccctc ttgataaccc aagagggcat tttttacgat aaagaagatt tagcttcaaa
     3301 taaaacctat ctattttatt tatctttcaa gctcaataaa aagccgcggt aaatagcaat
     3361 aaattggcct tttttatcgg caagctcttt taggtttttc gcatgtattg cgatatgcat
     3421 aaaccagcca ttgagtaagt ttttaagcac atcatcatca taagctttaa gttggttctc
     3481 ttggatcaat ttgctgacaa tggcgtttac cttaccagta atgtattcaa ggctaatttt
     3541 ttcaagttca ttccaaccaa tgataggcat cacttcttgg atagggataa ggtttttatt
     3601 attatcaata atataatcaa gataatgttc aaatatactt tctaaggcag accaaccatt
     3661 tgttaaatca gtttttgttg tgatgtaggc atcaatcata attaattgct gcttataaca
     3721 ggcactgagt aattgttttt tatttttaaa gtgatgataa aaggcacctt tggtcaccaa
     3781 cgcttttccc gagatctcat ctattgaaac agcttgatag cctttttcaa caaacaatat
     3841 tcgtgctgag ttaaccagtg attgataggt actcttaaaa ttttcttgtt gatgattttt
     3901 attttccatg atagatttaa aataacatac cgtcagtatg tttatggtat catgatgatg
     3961 tggtcgtgac aatcttaaga acatttaggt tattttatgt atattgaaca gcattctcgc
     4021 tatcaaaata aagctaataa catccaatta agatatgatg ataagcagtt tcatacaacg
     4081 gttatcaaag atgttctatt atggattgaa cataatttag atcagtcttt actgcttgat
     4141 gatgtggcga ataaagcggg ttataccaag tggtattttc agcggctgtt caaaaaagta
     4201 acaggggtca cactggctag ctatattcgt gctcgtcgtt tgacgaaagc ggctgttgag
     4261 ttgaggttga cgaaaaaaac tatccttgag atcgcattaa aatatcaatt tgattcccaa
     4321 caatctttta cacgtcgatt taagtacatt tttaaggtta caccaagtta ttatcggcgt
     4381 aataaattat gggaattgga ggcaatgcac tgagagatcc cctcataatt tccccaaagc
     4441 gtaaccatgt gtgaataaat tttgagctag tagggttgca gccacgagta agtcttccct
     4501 tgttattgtg tagccagaat gccgcaaaac ttccatgcct aagcgaactg ttgagagtac
     4561 gtttcgattt ctgactgtgt tagcctggaa gtgcttgtcc caaccttgtt tctgagcatg
     4621 aacgcccgca agccaacatg ttagttgaag catcagggcg attagcagca tgatatcaaa
     4681 acgctctgag ctgctcgttc ggctatggcg taggcctagt ccgtaggcag gacttttcaa
     4741 gtctcggaag gtttcttcaa tctgcattcg cttcgaatag atattaacaa gttgtttggg
     4801 tgttcgaatt tcaacaggta agttagttgc tagaacccat ggctcctttg ccgacgctga
     4861 gtagatttta ggtgacgggt ggtgacaatg agtccgtgtc gagcgctgat tttttcggcc
     4921 tttagagcga gatttataca atagaatttg gcatgagatt ggattgcttt tagtcagcct
     4981 cttatagcct aaagtctttg agtgactaga tgacatatca tgtaagttgc tgataggttt
     5041 ccagttttcc gctcctaggt ctgcatattg tacttttcct cttactcgac ttaaccagta
     5101 ccaacccagc ttctcaacgg atttatacca tggcacttta aagccagcat cactgacaat
     5161 gagcggtgtg gtgttactcg gtagaatgct cgcaaggtcg gctagaaatt ggtcatgagc
     5221 tttctttgaa cattgctctg aaagcgggaa cgctttctca taaagagtaa cagaacgacc
     5281 gtgtagtgcg actgaagctc gcaataccat aagtcgtttt tgctcacgaa tatcagacca
     5341 gtcaacaagt acaatgggca tcgtattgcc cgaacagata aagctagcat gccaacggta
     5401 tacagcgagt cgctctttgt ggaggtgacg attacctaac aatcggtcga ttcgtttgat
     5461 gttatgtttt gttctcgctt tggttggcag gttacggcca agttcggtaa gagtgagagt
     5521 tttacagtca agtaatgcgt ggcaagccaa cgttaagctg ttgagtcgtt ttaagtgtaa
     5581 t
//
LOCUS       DAEBXN010000193         5560 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.194, whole genome shotgun sequence.
ACCESSION   DAEBXN010000193 DAEBXN010000000
VERSION     DAEBXN010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5560
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.194"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..644
                     /locus_tag="KJL99_004089"
     CDS             <1..644
                     /locus_tag="KJL99_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HBD4438616.1"
                     /translation="VVGACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGCGGLGAIT
                     TGKDSQGKLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLRAFPRHIPAQN
                     AVLDVTFEFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCA
                     LGLSRSALVVAAWLLCYGHCKTVDEAIIFIRARRSHIVLKEEHKAMLKLWENR"
     gene            complement(698..1303)
                     /gene="azoR"
                     /locus_tag="KJL99_004090"
     CDS             complement(698..1303)
                     /gene="azoR"
                     /locus_tag="KJL99_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HBD4438617.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWSEKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            1504..5406
                     /gene="hrpA"
                     /locus_tag="KJL99_004091"
     CDS             1504..5406
                     /gene="hrpA"
                     /locus_tag="KJL99_004091"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HBD4438618.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHCVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVTIKLFDNPLEQKQAMWNGLRRLLLLNISSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
BASE COUNT         1377 a         1360 c         1540 g         1283 t
ORIGIN      
        1 atgtcgtagg cgcgtgcatt gtgttgatgg agctaatgat gatggttcag ttatggtggt
       61 cagtctggtt atgttggcca gtattatcgc tactcattat tggccgtggg tgcggtgggc
      121 ttggcgcgat aacaacaggg aaagatagtc aggggaaact cccgcccgcc gtttactggc
      181 tgacattgcc ctggcgcatc gggatgtggc tatctatgcg ttggttttgt cgtcgcctgg
      241 agccggtgag caaaatgact gctggtgttt atttaagggc gtttccacga catattccgg
      301 cacagaatgc ggttctggac gtcacctttg aattccctcg tggacgagcg acaaaagatc
      361 gactctattt ctgtgtaccg atgttggatc tggtggttcc ggaagagggg gagctccgac
      421 aggccgtggc gatgctggaa acattacgcg aagagcaagg cagcgttctg gtccattgcg
      481 cattgggatt atcgcgcagt gcgctggtag tggcggcatg gctgttatgt tacggacact
      541 gtaaaacagt tgatgaagcg attatcttta ttcgagccag acgctcgcat attgtgctta
      601 aggaagagca caaagcgatg ttgaaattat gggaaaacag gtaagtggat tgagatgtgg
      661 actgaatatc tacagtccac atcaagaccg tgtccggtta tgcagcaaca atgctgtcga
      721 tggctgcttt tgcgtcagac tgtgctttcg ctgccatttc cggaccgtat gcgatccctt
      781 cggcgaagac aaatttcaca tcggtaatgc cgataaagcc gaggaacgtg gacagatacg
      841 gcgtcaccag gtccgttggt ccatctttgt ggatcccacc gcggctggta ataacgatgg
      901 cttttttacc cgttaccaga ccttccggac cgttctcggt atagcggaaa gtaacgcctg
      961 cgcgtgcaac caggtcaaaa tagtttttca actgagttga gatgttgaag ttatacatcg
     1021 gtgccgcaat aacgataacg tcgtgggctt tcagctcggc aatcagctca tcggaaagcg
     1081 ccagagcttc ctgctgacgc ggagtcagcg gcgcatcgct cggacgcaga gcgccaacca
     1141 gttcgccatc cagtaccgga atcggatttg cagctaggtc gcgaacggtg atttcatcag
     1201 cggagtgctt ttcgctccat tgttcaacaa aataatcgga caactgatta gactgagagt
     1261 accctgccag gatgctggat ttaagaacta ataccttgct catggtgttt ccttatagat
     1321 gtttgaatgg acgatgcccc gttgcttgct gacactttat tcacaatcct gccacagaga
     1381 tcgcgcaata aatcgaagtc tatgttcgaa tttattgaac aacgcataga aagccgcgat
     1441 gtggtactct atatctatca tttaaaagaa aattaatcag gcagactact gcccactaac
     1501 gttatgacag aacaacaaaa attgaccttt actgccttgc aacagcggct ggattcgctg
     1561 atgctgcgcg acagactgcg tttttctcgc cgtctgcatt gtgttaagaa ggttaaaaat
     1621 cctgatgcac aacaggccat tttccaggag atggcgaaag agattgacca ggcggcaggg
     1681 aaagtcctgc tgcgtgaagc ggcacgaccg gaaatcactt atcctgacaa tttaccggtt
     1741 agtcagaaaa aacaggacat actcgaagcg attcgtgatc accaggtggt gatcgtcgcc
     1801 ggggaaaccg gctccggtaa aacgactcag ctaccgaaaa tctgtatgga gctggggcgc
     1861 ggtattaaag ggctgatcgg tcatacccag ccgcgtcgcc tggcggcacg aacggtggcc
     1921 aaccgtattg cggaagagct gaaaacggag ccgggcggtt gcatcggtta caaagtgcgt
     1981 ttcagcgatc acgtaagtga taacacgatg gtcaagctga tgaccgacgg gatcctgctg
     2041 gcggagatcc agcaagaccg cctgctgatg cagtacgaca ccatcattat tgacgaagcg
     2101 cacgaacgca gcctgaatat cgattttctg ctcggctatt tgaaagagtt gctgccgcgg
     2161 cgtcctgacc taaaaatcat tatcacttcc gcgactatcg acccggaacg cttttcgcgc
     2221 cactttaata atgcgccgat tattgaagtc tccggtcgga cctatccggt ggaagtgcgc
     2281 tatcgcccga ttgttgaaga agccgatgac accgagcgcg atcagttgca ggcgattttt
     2341 gacgccgtag acgaactaag ccaggaaagc cccggcgaca ttctgatctt tatgagcggc
     2401 gagcgggaaa ttcgcgatac cgccgatgcg ctgaacaagc tgaacttacg ccataccgaa
     2461 atcttgccgc tttatgcgcg gctttcgaac agcgaacaga accgcgtgtt ccagtcgcac
     2521 agcggacggc gcattgtgct ggcgaccaac gtcgcggaaa cctcgctgac cgtaccgggg
     2581 attaaatacg tcatcgaccc cggtacagcg cgtatcagcc gctacagcta tcgcaccaaa
     2641 gtgcagcgtt tgccgattga acccatttcg caggcgtctg ccaatcagcg taaaggccgc
     2701 tgtggtcgtg tgtccgaagg gatctgtatt cgtctctatt ccgaagacga tttcctctcg
     2761 cgcccggagt ttaccgatcc ggagattctg cgtaccaacc tggcctcggt tattttgcag
     2821 atgaccgcgc tggggctggg cgatatcgct gcgttcccgt ttgtcgaagc accggataaa
     2881 cgcaatatcc aggatggcgt gcgtctgctc gaagaactgg gcgcgatcac cacggatgaa
     2941 caggccagcg cctataaact gacgccgctc ggtcgccagc tctcgcagtt gcctgtcgac
     3001 ccacgtctgg cgcgtatggt gctggaagcg caaaaacatg gctgcgtgcg tgaggcgatg
     3061 attatcacgt ccgcgctctc catccaggat ccgcgcgaac gtccgatgga caaacagcag
     3121 gcatcggacg aaaaacatcg tcgcttccac gacaaagaat ccgacttcct cgcgtttgta
     3181 aatctgtgga attatcttgg cgagcagcaa aaggcgcttt cttccaacgc cttccgtcgc
     3241 ctgtgtcgta ccgattacct caactatctg cgcgtgcgcg aatggcagga tatctacacc
     3301 cagttgcgcc aggtggtgaa agaacttggc attccggtca acagcgaacc ggcggagtat
     3361 cgtgaaattc acatcgcctt actgaccggt ttgctttccc atatcggcat gaaagatgcc
     3421 gataaacagg aatataccgg tgcacgtaac gcgcgtttct ccatcttccc cggttctggt
     3481 ttattcaaaa agccgccgaa atgggtaatg gtggcggaac tggtagaaac cagccgcctg
     3541 tgggggcgca ttgctgcgcg tatcgacccg gaatgggtag aacccgttgc tcagcatttg
     3601 attaaacgca cctacagcga accgcactgg gaacgggcgc agggcgcggt gatggcaacg
     3661 gaaaaagtca ctgtttatgg tttgccgatt gttgctgcgc gcaaggtcaa ctacagccag
     3721 atcgattcgg cgttatgtcg tgaactcttt attcgccacg ctctggtgga aggtgactgg
     3781 cagacgcgtc acgcattctt ccgtgaaaac ctgaaactgc gggccgaagt ggaagagctg
     3841 gaacacaaat cacgtcgccg cgatattctg gttgatgacg aaacgctgtt tgagttctac
     3901 gaccagcgca tcagccacga tgtaatctcc gctcgtcact tcgatagctg gtggaaaaaa
     3961 gtcagccgcg aaacgcctga tttgctcaac tttgaaaaga gcatgttgat caaagaaggg
     4021 gcggaaaaaa tcagcaagct ggattacccg aacttctggc atcagggcaa tctcaagctg
     4081 cgtttgagct atcagtttga gccaggcgcg gacgcggacg gtgtgaccgt acatattccg
     4141 ctgccgttac ttaaccaggt tgaggaaagc gggtttgaat ggcagatccc cggtctgcgc
     4201 cgcgaattgg tgattgctct gattaaatcg ttgccgaaac cggtacgccg taattttgtg
     4261 cctgcaccaa actacgccga agcgttttta ggccgcgtca caccgctgga gttaccgttg
     4321 ctcgacagcc ttgagcgcga gttacggcgg atgaccggcg ttaccgttga ccgcgaagac
     4381 tggcactggg atcaggtgcc cgatcacctg aaaatcacct tccgcgtggt ggatgacaaa
     4441 aacaagaagc taaaagaagg gcgctcgcta caagatctga aagatgcgct gaaaggcaaa
     4501 gtgcaggaaa cgctgtccgc ggtggcggat gacggtatcg agcagagcgg cttacatatc
     4561 tggagttttg gtcagctgcc ggaaagttac gaacagaagc gtggcaacta taaagtgaag
     4621 gcgtggccgg cgctggtgga tgaacgcgac agcgtgacga tcaaactgtt tgataacccg
     4681 ctggagcaaa aacaggcaat gtggaatggt cttcgccgtc tactgctgct aaatatttcc
     4741 tcgccgatca agtatttaca tgagaagtta ccaaacaaag ccaagctggg gctgtacttt
     4801 aacccgtatg gcaaagtgct ggagctgatc gacgactgta tctcctgcgg tgtggatcaa
     4861 ctgatcgacg ccaatggtgg cccggtctgg acggaagaag gctttgctgc gctgcatgaa
     4921 aaagtgcgcg ccgaactgaa tgacacggtg gtggatattg cgaagcaggt cgagcaaatc
     4981 ctcactgcgg tgttcaatat caacaaacgt ctgaaagggc gggtggatat gaccatggca
     5041 ctggggcttt ctgatattaa agcgcagatg ggcgggttgg tatatcgcgg ttttgtcact
     5101 ggtaacggct tcaaacggct gggcgacacg ctgcgatatt tgcaggcgat tgaaaaacga
     5161 ctggaaaaac tggcggttga tccacatcgc gatcgtgcac agatgctgaa agtcgaaaac
     5221 gtccagcagg cgtggcagca atggatcaac aaactgccgc ctgcacgtcg tgaggatgaa
     5281 gacgtgaaag agatccgttg gatgatagaa gagctgcgcg ttagttactt cgctcaacaa
     5341 cttggtacgc cttatccgat ttcagataag cgtattttgc aggcgatgga gcagattagc
     5401 ggttaaccct gctatttgcc taataaatct aaaacccggt aagcatttag cgccgggttt
     5461 tttattaatt ctaaaacggt aaggacaaaa attcaggaat tcagaaaaat acaattctca
     5521 gctgcaagat gaataatgtt tatctacagc atttccttaa
//
LOCUS       DAEBXN010000194         5547 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.195, whole genome shotgun sequence.
ACCESSION   DAEBXN010000194 DAEBXN010000000
VERSION     DAEBXN010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5547
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.195"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            6..407
                     /locus_tag="KJL99_004092"
                     /pseudo
     CDS             6..407
                     /locus_tag="KJL99_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            572..4690
                     /gene="pic"
                     /locus_tag="KJL99_004093"
     CDS             572..4690
                     /gene="pic"
                     /locus_tag="KJL99_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708747.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Pic"
                     /protein_id="HBD4438619.1"
                     /translation="MNKVYSLKYCPVTGGLIAVSELARRVIKKTCRRLTHILLAGIPA
                     ICLCYSQISQAGIVRSDIAYQIYRDFAENKGLFVPGANDIPVYDKDGKLVGRLGKAPM
                     ADFSSVSSNGVATLVSPQYIVSVKHNGGYRSVSFGNGKNTYSLVDRNNHPSIDFHAPR
                     LNKLVTEVIPSAVTSEGTKANAYKYTERYTAFYRVGSGTQYTKDKDGNLVKVAGGYAF
                     KTGGTTGVPLISDATIVSNPGQTYNPVNGPLPDYGAPGDSGSPLFAYDKQQKKWVIVA
                     VLRAYAGINGATNWWNVIPTDYLNQVMQDDFDAPVDFVSGLGPLNWTYDKTSGTGTLS
                     QGSKNWTMHGQKDNDLNAGKNLVFSGQNGAIILKDSVTQGAGYLEFKDSYTVSAESGK
                     TWTGAGIITDKGTNVTWKVNGVAGDNLHKLGEGTLTINGTGVNPGGLKTGDGIVVLNQ
                     QADTAGNIQAFSSVNLASGRPTVVLGDARQVNPDNISWGYRGGKLDLNGNAVTFTRLQ
                     AADYGAVITNNAQQKSQLLLDLKAQDTNVSEPTIGNISPFGGTGTPGNLYSMILNSQT
                     RFYILKSASYGNTLWGNSLNDPAQWEFVGMNKNKAVQTVKDRILAGRAKQPVIFHGQL
                     TGNMDVAIPQVPGGRKVIFDGSVNLPEGTLSQDSGTLIFQGHPVIHASISGSAPVSLN
                     QKDWENRQFTMKTLSLKDADFHLSRNASLNSDIKSDNSHITLGSDRAFVDKNDGTGNY
                     VIPEEGTSVPDTVNDRSQYEGNITLNHNSALDIGSRFTGGIDAYDSAVSITSPDVLLT
                     APGAFAGSSLTVHDGGHLTALNGLFSDGHIQAGKNGKITLSGTPVKDTANQYAPAVYL
                     TDGYDLTGDNAALEITRGAHASGDIHASAASTVTIGSDTPAELASAETAASAFAGSLL
                     EGYNAAFNGAITGGRADVSMHNALWTLGGDSAIHSLTVRNSRISSEGDRTFRTLTVNK
                     LDATGSDFVLRTDLKNADKINVTEKATGSDNSLNVSFMNNPAQGQALNIPLVTAPAGT
                     SAEMFKAGTRVTGFSRVTPTLHVDTSGGNTKWILDGFKAEADKAAAAKADSFMNAGYK
                     NFMTEVNNLNKRMGDLRDTNGDAGAWARIMSGAGSADGGYSDNYTHVQVGFDKKHELD
                     GVDLFTGVTMTYTDSSADSHAFSGKTKSVGGGLYASALFESGAYIDLIGKYIHHDNDY
                     TGNFASLGTKHYNTHSWYAGAETGYRYHLTEDTFIEPQAELVYGAVSGKTFRWKDGDM
                     DLSMKNRDFSPLVGRTGVELGKTFSGKDWSVTARAGTSWQFDLLNNGETVLRDASGEK
                     RIKGEKDSRMLFNVGMNAQIKDNMRFGLEFEKSAFGKYNVDNAVNANFRYMF"
     gene            complement(4841..5083)
                     /locus_tag="KJL99_004094"
                     /pseudo
     CDS             complement(4841..5083)
                     /locus_tag="KJL99_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025243830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(5122..>5547)
                     /locus_tag="KJL99_004095"
     CDS             complement(5122..>5547)
                     /locus_tag="KJL99_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBD4438620.1"
                     /translation="AEQRLAARKARAAPLMQSLYDRIQQQMKTLSRHSDTAKAFAYLL
                     KQWKALNVYCSNGWVEIDNNIAENALRAVSLGRKNFLFFGSDHGGERGALLYSLIGTC
                     KLNDVDPESYLRHVLGVIADWPVNRVSELLPWRIALPAE"
BASE COUNT         1457 a         1288 c         1482 g         1320 t
ORIGIN      
        1 ggtgacgagt ctcatcgtct atggaaacga cattcgtgct ggatgcactg gagcaggcgt
       61 tgtgggcccg tcgtccgtct ggcaccatcc atcacagcga taaaggctct cagtatgtgt
      121 cactggccta taaggcgcga ctaaaagaag caaaactgct ggcatcaaca gggagtactg
      181 gtgactcgta tgacaacgcg atggcagaga tcatcaaggg cctttacaaa gcggaggtaa
      241 tacaccgtaa gagctggaaa aaccggacag aagtggagct ggccacactc acgtgggtgg
      301 actggtataa caatcgacga ttgctggaaa ggctgggcca tatcccaccg gcagaagcag
      361 aaaaagctta ttatgcttcc atcggaaatg atgatctggc agcctgagtt cacagataaa
      421 acactctcca ggaaacccgg ggcggttcag ttcacaaaaa cacattaatg cagtaactat
      481 attttccttt ctggtgataa cgtcgggtta tcattagctt cttcagctat tttactttta
      541 tatcccttgt aaacatcatg gagaatccat agtgaataaa gtttattctc ttaaatattg
      601 ccccgtcacc ggggggctta ttgctgtctc tgaacttgcc cgcagggtaa taaaaaagac
      661 atgccgaaga ttaacgcata ttcttctggc tggcattcca gcaatctgtc tgtgttactc
      721 tcagatatcc caggcgggta ttgtccgttc cgatattgcc tatcagattt atcgtgattt
      781 cgccgaaaac aaagggcttt ttgtacctgg tgccaatgat attccggtat atgataagga
      841 cggaaaactt gtgggaagac tgggtaaagc cccaatggcc gatttcagca gtgtgagctc
      901 aaatggcgtt gctacgcttg tatcgcctca gtatatcgtc agcgtaaagc ataacggagg
      961 atatcggagt gtgagctttg gtaatgggaa aaatacatat tcccttgttg accgtaataa
     1021 ccacccttct attgacttcc atgctccacg tctgaataaa ctggttacag aagttattcc
     1081 ctcagcggta acatcagaag gaaccaaagc caatgcttat aaatacactg aacgttacac
     1141 cgctttttat cgggtgggta gtggtacgca gtacactaag gacaaggacg gaaatttagt
     1201 taaggttgcc ggtggatatg cttttaaaac aggaggaacc acaggagttc ctctgatatc
     1261 tgatgcaaca atagtctcta atcccgggca aacttataat cctgtaaacg gccctttacc
     1321 tgactatgga gcccctgggg atagtggttc tcctttgttt gcttatgata aacaacaaaa
     1381 aaaatgggtt attgttgctg tattaagagc atatgcaggt attaatggtg ctacgaactg
     1441 gtggaatgtc ataccaacag attatctgaa ccaggttatg caggacgatt tcgatgcccc
     1501 cgtagacttt gtttccggac tgggccccct gaactggaca tacgacaaaa catcaggcac
     1561 aggtaccctg agccagggca gtaaaaactg gaccatgcac gggcagaaag acaatgacct
     1621 caatgccggt aaaaatctgg tattcagcgg gcagaatggt gcaattatcc tgaaagacag
     1681 tgtgactcag ggtgccggtt atctcgaatt taaagacagt tacaccgtat ctgctgaatc
     1741 cggaaaaaca tggacgggtg ccggcattat tactgacaag gggacgaatg taacctggaa
     1801 ggtcaacggc gttgccggtg acaacttgca taagctgggg gaaggaaccc tgaccataaa
     1861 cggaacaggt gtaaacccgg gaggactgaa aacgggagac ggtatcgttg tacttaacca
     1921 gcaggcagac actgcaggta atatccaggc cttcagttca gtgaacctcg ccagcggacg
     1981 tccgaccgtg gtgctcgggg atgcccgtca ggtcaatccg gataacattt catggggata
     2041 ccggggaggt aagcttgacc ttaatggtaa tgccgttacc ttcacccgac tgcaggctgc
     2101 tgattacggg gcggtgatta caaataatgc acagcaaaaa tcccagcttt tactggatct
     2161 taaggctcag gatacaaatg tcagtgaacc gacgattgga aatatatccc cctttggtgg
     2221 taccggcact ccaggaaacc tgtacagcat gatactcaac agccagaccc gcttctatat
     2281 tctgaaatct gccagctatg gtaacactct gtgggggaac agcctgaatg atccggctca
     2341 gtgggagttt gttggcatga acaaaaacaa agcagttcag acagtaaaag ataggatcct
     2401 ggccgggcgg gcaaaacaac ccgttatctt tcatggtcag ctgaccggga atatggatgt
     2461 cgccattcca caggtgccgg ggggaagaaa ggtaatcttt gatggtagcg tgaacctgcc
     2521 ggaaggtacc ctgagtcagg acagtggcac cctgatattc cagggacatc cggttatcca
     2581 tgcctccatc agtggcagtg caccggtcag cctgaaccag aaagactggg aaaaccgtca
     2641 gtttacaatg aaaacactgt cgctgaaaga cgctgacttc catctttcac gtaacgcctc
     2701 gctgaacagt gacattaagt cggataacag ccatatcaca ctgggaagtg acagggcatt
     2761 tgtggataaa aatgacggaa caggaaatta tgtcattccg gaggaaggta cctctgtccc
     2821 ggacaccgtg aatgacagga gccagtatga agggaatatt acgctgaacc ataactcagc
     2881 cctggatatc ggcagcaggt tcaccggggg gattgacgct tatgacagtg ccgtcagcat
     2941 cacctctccg gacgtcctgt tgacagcccc gggtgctttt gccggcagtt cactgacagt
     3001 gcatgatggc ggtcatctta cagcactgaa cggtcttttc agcgacgggc atattcaggc
     3061 cggtaagaac ggcaaaatca ccctgagcgg tacaccggtt aaagatacgg ctaatcagta
     3121 tgcccctgct gtatatctga cggacggata tgacctgacc ggcgataacg cagcactgga
     3181 aattacccgt ggagcacatg cttccggtga tattcatgcc tctgcggcat caacagttac
     3241 catcgggtct gacacgccgg cagaactggc ttctgcggaa acggctgcat cggcgtttgc
     3301 cggcagtctt cttgagggct ataacgcagc attcaatggt gccataaccg gtggcagggc
     3361 tgatgtcagt atgcataatg cactgtggac tctgggtggg gactctgcca tccacagtct
     3421 taccgtcaga aacagccgta ttagttctga aggagaccgt acattccgta ccctgacggt
     3481 gaataaactg gatgcaacag gcagtgattt tgttttgcgt acggacctga aaaatgccga
     3541 taaaattaat gtgactgaaa aagccactgg ttcagataac agcctgaacg tcagctttat
     3601 gaataatcct gctcagggac aggccctgaa tattcctctg gtcacggcac cggcgggaac
     3661 ttcagcagag atgtttaagg ccggcacccg ggtgacaggt ttcagtcggg tgaccccaac
     3721 cctgcatgtt gataccagtg gtggcaatac gaagtggata ctggatggtt ttaaagcgga
     3781 ggctgataaa gccgctgccg cgaaggctga cagtttcatg aatgccgggt ataaaaactt
     3841 catgacggaa gttaacaatc tgaacaaacg tatgggtgac ctgcgtgaca caaacggtga
     3901 tgccggtgcc tgggcgcgca tcatgagtgg tgccggttct gcagacggtg gttacagtga
     3961 taattacacc catgttcagg tcggctttga caaaaaacat gaactggacg gtgtggacct
     4021 gtttaccggt gtcacgatga cctataccga cagcagtgca gacagccatg cattcagcgg
     4081 aaagacgaaa tcggtggggg gcggtctgta tgcttcagca ttgtttgagt ccggtgccta
     4141 tatcgatttg attggtaaat atattcacca tgacaatgat tacacaggta actttgctag
     4201 cctgggaacg aaacactaca acacccattc ctggtatgcc ggtgctgaaa cgggttaccg
     4261 ctatcacctg acagaggaca cgttcattga gccgcaggct gaactggttt acggcgccgt
     4321 gtccgggaaa acattccgct ggaaagacgg tgatatggac ctgagcatga agaacaggga
     4381 cttcagtccg ctggttggaa gaacaggggt tgaactgggc aagaccttca gtggtaagga
     4441 ctggagtgtg acggcccgtg ccggaaccag ctggcagttt gacctgctga ataatggaga
     4501 gaccgtactg cgtgatgcgt ccggggagaa acggataaaa ggagagaagg acagccggat
     4561 gctgtttaat gttggtatga atgcgcagat aaaggacaat atgcgctttg gtctggagtt
     4621 tgagaagtca gcctttggta aatataacgt ggataatgcg gtaaacgcga atttccggta
     4681 tatgttctga tgtgggggta aagggctgcc ggaaggcagc ctttctcaga gactcttaaa
     4741 atataaaact gaaggtcttc ggtaaggggg agtgtattag acgtcaacta gttttgccga
     4801 ctggcgcgag cctgtttaag attctgtgta aatgcctttc tcagaagtga ccgtccaggc
     4861 ggtcaccgaa ctcgataata aagcggctca ttgccattcg ccagtccttc aacggcatcg
     4921 tccatttctg ggacgcagac tggattgcca gccacaccac ttttttcacc gagtcgtctg
     4981 tcgggaacac tttacgcttt ttgatcgcat ggcggatcac gctgtttagc gactcgatgg
     5041 cattcgtcgt atagatcact ttgcggatgt ccgttggata agcggtaagc gtacagcgag
     5101 ggccgtattg acggggatgt gttattcagc tggcagtgct atgcgccacg gaagcagttc
     5161 gctgacccgg ttgaccggcc agtctgctat gacgccaagc acatggcgaa ggtagctttc
     5221 tggatccacg tcattcagtt tgcacgtccc gatcaggctg tacagtagcg cgccccgctc
     5281 accaccatgg tcagagccga agaacaggaa gtttttacga cccagactga ccgcccgcag
     5341 ggcattttca gcgatgttgt tgtcgatttc cacccagcca ttactgcagt acacgttcag
     5401 ggccttccac tgtttcagta ggtatgcgaa cgcttttgcc gtatccgagt gacgcgacag
     5461 tgttttcatc tgttgctgta tccggtcata cagtgactgc atcagtggcg cggctcttgc
     5521 ttttcttgcc gccagacgct gttctgc
//
LOCUS       DAEBXN010000195         5524 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.196, whole genome shotgun sequence.
ACCESSION   DAEBXN010000195 DAEBXN010000000
VERSION     DAEBXN010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5524
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.196"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(150..511)
                     /locus_tag="KJL99_004096"
                     /pseudo
     CDS             complement(150..511)
                     /locus_tag="KJL99_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(850..1404)
                     /locus_tag="KJL99_004097"
                     /pseudo
     CDS             complement(850..1404)
                     /locus_tag="KJL99_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
     gene            1547..1669
                     /locus_tag="KJL99_004098"
                     /pseudo
     CDS             1547..1669
                     /locus_tag="KJL99_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1931..2497)
                     /gene="ipgB2"
                     /locus_tag="KJL99_004099"
     CDS             complement(1931..2497)
                     /gene="ipgB2"
                     /locus_tag="KJL99_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB2"
                     /protein_id="HBD4438621.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSRKIGDNLRKQIFKQV
                     EKDYRISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(2779..3093)
                     /locus_tag="KJL99_004100"
     CDS             complement(2779..3093)
                     /locus_tag="KJL99_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438622.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSVQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            complement(3171..3281)
                     /locus_tag="KJL99_004101"
                     /pseudo
     CDS             complement(3171..3281)
                     /locus_tag="KJL99_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(3605..4282)
                     /gene="ospD1"
                     /locus_tag="KJL99_004102"
     CDS             complement(3605..4282)
                     /gene="ospD1"
                     /locus_tag="KJL99_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector/anti-activator
                     OspD1"
                     /protein_id="HBD4438623.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMKNNIINVTNTAIS
                     HAINEEKSGGGYSGVSFRKLAKIQNISIPTKNNKEYNRHNLFSLIWHGNADAARKYGE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGLPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            complement(4654..4791)
                     /locus_tag="KJL99_004103"
                     /pseudo
     CDS             complement(4654..4791)
                     /locus_tag="KJL99_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(4873..5170)
                     /locus_tag="KJL99_004104"
                     /pseudo
     CDS             complement(4873..5170)
                     /locus_tag="KJL99_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343747.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1500 a         1197 c         1108 g         1719 t
ORIGIN      
        1 ccggctactc attccctgct tgtgcaggat tttaatttcc ataactgtct caaaagtgac
       61 cataagctct cctgaatcag gagagcagat taccccctgg atctgatttc aggcgttggg
      121 tgtggatcac tattgcaccg ttcgtgacag tgttgttgcg ccttgctgcc aacgaagctc
      181 cactgcccat caagttcaca gataagggca acatcagcgt gagcgactgg cgcagatgtt
      241 atccgccggg gcgggagctt tttaatgtcc gtatgacggt attgatgccg attttcagcg
      301 tacggccagt atcgcgaact ccggcaccgt tgaaagccat ttcagtgatt tggtttttaa
      361 ctcccggttt acgggcctcg taacagtaag tgagctgaaa cacccggtga cagtcacggc
      421 aacggaatcg atcatgtcct tttgggttct gcccgtgacg gtaaacctga acggactggc
      481 aacgggggca atgaaagtta actgtgacca tgaaagaacc taaaagcggg tattataacc
      541 cctaattcaa ctaattggag gcattgtcat ttttaatcta tggatacact taaaaatcct
      601 ctcaccttac gttaaaaacc ttctcatgtt tagagttttt tattatgttt atcataatga
      661 tatgacgatc tgagttttta aaatcaagaa gaaaagataa gttttttaaa tgcacagtgc
      721 ctgtggataa ctttgcgtaa atccggagga cagatcactc tgaagcaaga caacagcatc
      781 tgaaaacaat atgctcacgt ttcaaccaca gggcgtaagt gatcctaccc acgtaatatg
      841 gacacaggcc taagcgaggt tctggttttc aaattgttcc ggactgaggc cgccacacca
      901 actgtgccgt cgccagcgat tgtaatcaca ttcgatataa ttaaacactg ttgccctcat
      961 tatttcccgg ctgataaagt gttctccgtg gagacattcc actttcagcg aatgaaagaa
     1021 gctttccacg caggcattat cgtagcagca gccctttgcg ctcatactgc cacgcagatt
     1081 atgccacttc agcagcgcct gataatctgc tgaacagtac tgacttcctc ggtccgagtg
     1141 aacgataacg ttccggggtc tcttacgccg ccacagcgcc atctgcaggg catcgcaggc
     1201 cagttgcgcc gtcaggcgtg gtgacatgga ccagccaata acggcacgtg accacaggtc
     1261 aatgaccaca gccagataca gccagccttc atctgtacgt aagtacgtga tgtctcccgc
     1321 ccacttctgg ttcggaccac tggcgtaaaa atcctgctcc agcagatttt ctgacacagg
     1381 caggccatgt gcgcggtaac tgaccgtatt gctgggtaag atcaatttgc tcactggtat
     1441 ttactacgtg catcgaatga gtgatcaaga gccgtatgac gcgagagtgt cacgtacggt
     1501 tccgtaagca gccggagagt gaaactctct ccggctgact cgactcgcag gttccgacag
     1561 cggtgacgaa catgcagcgg tgttgttctc gctgatcggc acatgccgtc tgaacaatgt
     1621 ggagccagaa aaatggctgc gttacgttat tgaacatatc caggactgac cggcaaaccg
     1681 ggtacgcgat ctgttgcctt ggaaagttga tctgacttct cagtaaatat caatatgatt
     1741 ctgacgagcc gcttaccttt gagcagagaa aggggagagg gatcccacat ccatgagcta
     1801 ggaaaataca aaaactggct atacaacaat tcaatatatt aggacttact tgcgaggaga
     1861 aatatagcac cgctcaggca attcctgggt atttactttt cttagtttct ttactcggga
     1921 tgttctgtca tcagaaaggc gattctaaat ttgtaatata gtcactcata ttcccacgta
     1981 atttctttag atcaatatat tgcacatttt tctcaaaaaa ctgatcggaa ataagatttg
     2041 caacattgct tgttgtatca tttttaacgc ttctattcat cccttcacaa agttcgtcca
     2101 ttttcttttt gaaataactg cttcctgata caagatgatt tatggaactt tgtgcagcat
     2161 tgatgtctag agaaatgcga taatctttct caacctgttt aaatatctgt ttacggagat
     2221 tatcgccgat ttttcggctg tgttctgcac aaaacttatc aatacattgg tttaccaccc
     2281 gggatacgta gacttttttt tcatcatttt tccaatctgt aatattttca ccgatttgtt
     2341 ctgaaacaga gctcaaaata ctagtattta ttttggttga tgatattttt actatccttt
     2401 tccccattgt acagcggata atctcatcca cctttcctga aaaatatctt ttgtgagata
     2461 aactgattcc aaaattatta aaagatgttc caagcattat aatcaccttg tcatgtaaaa
     2521 tagtaacatt gtcattattt tagctgattc aaacaattct gtaaatgctt tgagttcttt
     2581 actcccttgg ctcaatgatg gaccgtctgg aatcccagag actaaactct ctgagaaaga
     2641 gagtatgaaa aaacttgggg cagtccctaa atgaagggac caacagcggt aaaataaccg
     2701 gcatcattca taatgatgcc ctgttagttg tagttatgaa gatttacgtc attaaaattg
     2761 ctgttcgtag tgtcagtcct atggtctggc atcagctgag gatcgtagcc gatacatcac
     2821 tggcttcacg acattcgtgt tgaggctatt catgaaaact ccacgctgaa aacgccgttt
     2881 tgtataaacg gtcacggtat gcccggagcc actgttgctg ataaattcga taaaaccttg
     2941 gcgtttcttg aagctatcgt taatgcggat aatgaaacaa cgattggtga gattcgctct
     3001 tttgccgacg ccctggacgc tgtccggttc aatcgcaaca aaatcaaccg gcagctgagc
     3061 aaacccaacc ttgcctctct ggcgctggag catgaagtga tttggctggg acgtagccgc
     3121 tgacagcttc tataaaagta tgatcctacc cacgtaatat gaacacaggc ttaagtgagg
     3181 ttctggtttt caaattgttc aggactgaga cctccacacc aactgtgccg tcgtcagcga
     3241 ttgtaatcgc acttgatata attaaacact gtatatttca tttgtggagt ctccagtctg
     3301 agagggcttt atgcattccg tattgggcac tatgatgcca gaaatctgca aggctccaca
     3361 ccttcctgta tttcccttcc tctatcacag tgcactttac ttaatagggt gggatgtgtt
     3421 cattacattt attgtgtttt ttatgattgt ttttgcgtcc agtgtcgttc gtcatgggaa
     3481 actggttcta tgggtagtat cgctcagtct agttaactgt ttgtttcttt ttagaaatta
     3541 agcagatcac acaattcggg gagagatcct tctaagtgca attctttgca gctatttagc
     3601 acacttaaaa caactcttgt attttgtcag aaataatatt aaacatgtct tgttcattat
     3661 tgttaagtct gtctgctatt tctgtaagtt ttatcccact atgatggcat atatttatat
     3721 atgccatcat agcctcgata ttccctttcc ctgcggccag aaataatcct ggtaacccct
     3781 cattatctct tacatccaat agtttaatcg tttcttttgg tgataaatca aaagttttaa
     3841 taaaatctcc ataagcttga atcgcagcgg aatgaccttc ttgaagagct atgaacaaag
     3901 cagattcccc agcattattt cgtgctgcaa gaacttctag tttttcctct ttgggtattt
     3961 cggctgccaa cagcgattca ccgtatttac gcgctgcatc ggcatttcca tgccaaatca
     4021 atgaaaacaa attatggcgg ttatactcct tattattctt tgtcggaatg gatatgttct
     4081 gtattttggc caattttctg aaagaaacac cactatatcc cccccctgat ttttcttcat
     4141 tgatggcgtg ggatatagcg gtatttgtca cgttaatgat attatttttc attcctttat
     4201 tttcattagc agtattgctg cctattatta agtgcatatt tttgttgttt gctggatgta
     4261 atccatagtt atttattgac atttttgttc atcctttttg ctaaaaaaaa acaataacct
     4321 atttttgtaa tgtaatagat attagtctat tgctaatcta aaaaaaccat tgccacattg
     4381 aatggctata ttcaccaata aattctttcg atttcgtata ttgacatatc gtagatatat
     4441 cttatcccta agattcaata atcgagtccg tttaccattc tgtgcaagcg ccttcactca
     4501 gaagtgaccg tcaaggcgat caccaaactt gataataaag cagctcattg ccatacgcca
     4561 gtccttaagg ggcgttgtcc atttctgtga tgctgcctag atttaactgc cagctccggc
     4621 ttatctacat ctaaatatgt gagcctaata ccgctacact ttgtccagac tatgtttttc
     4681 tccgggaagt gggctaaccg tctccataaa gtgacgaact tttttcaaca gtggccatat
     4741 tgaacggcac tgatgattac gggttattgt ctcatgaaga gccggccaca tcactgacag
     4801 cagtgacttc aggccttcag caccagacag cctgaaccag ttaatccatc gcccgacaaa
     4861 tttagtaatc tagggctggt ttttcttcag ctgttgcggt tcgaaagtgc cattacgatc
     4921 acgcagagta cggatttcca gaggtcatct ccagtagtga taatcttcgt taagcaacca
     4981 ttgcgggaat tgctgccaga tctaagctga tttttctcgc acccaagatg atgagtcata
     5041 ttagcattga gtacggtttc attttcagca tccaatcaaa ctgactgaga tcttcaggat
     5101 ttttgagact tttggctagt tcattagcta aagcccgcaa cttattagat gtcaactgtt
     5161 tttcattcat aaattcacct tcatttgata tcaaattaaa tagttcaaca tcagagaatt
     5221 atacaaactt atgtacaggc tcctatgatc ttcgaatcct gaagattata gtgaaaataa
     5281 gttttcacgt tgaaatccca catagttagt atatgataaa taatcatgga tgacctgatt
     5341 aacctcttgg ggtagtgctt ttatagaaga ataaatattc tactaattta atacatcacc
     5401 taaaatagta ttgtttttta tggttggttg aaggtcgtgg tgatgatgtt agtccttttg
     5461 agaatattcc agtgccagat cttacttata ataaaggggc ttggcagttt ggataaacta
     5521 ttgt
//
LOCUS       DAEBXN010000196         5516 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.197, whole genome shotgun sequence.
ACCESSION   DAEBXN010000196 DAEBXN010000000
VERSION     DAEBXN010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5516
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.197"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            554..1543
                     /gene="araF"
                     /locus_tag="KJL99_004105"
                     /pseudo
     CDS             554..1543
                     /gene="araF"
                     /locus_tag="KJL99_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
     gene            1613..3127
                     /gene="araG"
                     /locus_tag="KJL99_004106"
     CDS             1613..3127
                     /gene="araG"
                     /locus_tag="KJL99_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HBD4438624.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLAIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIGNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKALGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEARKAEGIIPVHSVRDNINISARRKHVLGGCVINNSWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            3142..4128
                     /gene="araH"
                     /locus_tag="KJL99_004107"
     CDS             3142..4128
                     /gene="araH"
                     /locus_tag="KJL99_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HBD4438625.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            4295..5095
                     /gene="otsB"
                     /locus_tag="KJL99_004108"
     CDS             4295..5095
                     /gene="otsB"
                     /locus_tag="KJL99_004108"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HBD4438626.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITAALQQKRENNRSDDYESFSRSI"
     gene            5070..5516
                     /locus_tag="KJL99_004109"
                     /pseudo
     CDS             5070..5516
                     /locus_tag="KJL99_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310631.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
BASE COUNT         1360 a         1270 c         1473 g         1413 t
ORIGIN      
        1 tttttgtatc ttgttgatat taatcgatga atttgaattc atgatgaagt gatgatttta
       61 taaaacgttt tttcattttt gcgagatggc tctcattata cgtgttctgt taattaatga
      121 gcacagtgat aattaatggc gaaatgaatg acgcgcattt tccacatctt tgagttgtgg
      181 ttattacacc atttcaaaaa ctcaacgcca ggtaatgcgg cctattgact ggttaaaaag
      241 aagacatccc gcatgggtac cgaagacaac aaggatttcc aggctaatct tatggattaa
      301 tctgctgtgc attcaacaat ttgtctgaca aattggcttt tccttatgtc ttttcccgct
      361 aaatttatgc acgttctcac tgtaattctg cgatgtgata ttgctctcct atggagaatt
      421 aatttctcgc taaaactatg tcaacacggt cacttatctt ttagataaaa ggtaatgctt
      481 tgttttccga ttaatttaac gaatgtcatt cgtttttgcc ctacacaaaa cgacactaaa
      541 gctggagaga accatgcaca aatttactaa agccctggca gccattggtc tggcagccgt
      601 tatgtcacaa tccgctatgg cggagaacct gaagctcggt tttctggtga agcaaccgga
      661 agagccgtgg ttccagaccg aatggaagtt tgccgataaa gccgggaagg atttagggtt
      721 tgaggttatt aagattgccg tgccggatgg cgaaaaaaca ttgaacgcga tcgacagcct
      781 ggcagccagt ggcgcaaaag gtttcgttat ttgtactccg gacccgaaac tcggctctgc
      841 catcgtcgcg aaagcgcgtg gctacgatat gaaagtcatt gcagtggatg accagtttgt
      901 taacgccaaa ggtaagccaa tggataccgt tcctctggtg atgatggcgg cgactaaaat
      961 tggcgaacgt cagggctagg aactgtataa agagatgcag aaacgtggct gggatgtcaa
     1021 agaaagcgca gtgatggcga ttaccgccaa cgaactggat accgcccgcc gccgtacaac
     1081 gggttctatg gacgcactga aagctgcagg attcccggaa aaacaaattt atcaggtacc
     1141 taccaaatct aacgacatcc cgggggcatt tgacgctgcc aactcaatgc tggttcaaca
     1201 tccggaagtt aaacattggc tgatcgtcgg tatgaacgac agcaccgtac ttggcggcgt
     1261 acgtgcaacc gaaggtcagg gctttaaagc agccgatatc atcggtattg gtattaacgg
     1321 tgtggatgca gtaagcgaac tgtctaaagc acaggcaacc ggcttctacg gttccctgct
     1381 gccaagcccg gacgtacatg gctataaatc cagcgaaatg ctttacaact gggtagcaaa
     1441 agacgttgaa acgccaaaat ttaccgaagt tacagacgtg gtactgatca cgcgtgacaa
     1501 ctttaaagaa gaactggaga aaaaaggttt aggcggtaag taatttgccg gaaaaattcc
     1561 cctctgcatg atgcagaggg ggtgtgaacg accagtgatt cacggagacg ttatgcaaca
     1621 gtctaccccg tatctctcat ttcgcggcat cggtaaaacg tttcccggcg ttaaggcgct
     1681 gacggatatt agttttgact gctatgccgg tcaggttcat gcgttgatgg gtgaaaatgg
     1741 cgcaggaaaa tcaactctct taaaaatcct cagcggcaac tatgcgccaa ccacgggttc
     1801 tgtagtgatt aatgggcagg aaatgtcctt ttccgacacg accgcagcac ttaacgcggg
     1861 tgtggcgatt atttaccagg aactgcatct cgtgccggaa atgaccgtcg cggaaaacat
     1921 ctatctcggc cagctgccgc ataaaggcgg cattgtgaat cgctcattgc tgaattatga
     1981 ggcgggttta caacttaaac atcttggtat ggatattgac ccggacacgc cgctgaaata
     2041 tctcgccatt ggtcagtggc agatggttga aatcgccaaa gcgctggcgc gtaacgctaa
     2101 aattatcgcc tttgatgagc caaccagctc cctttctgcc cgcgaaatcg gcaatctttt
     2161 ccgcgttatt cgtgaactgc gaaaagaggg gcgggtgatc ttatacgttt ctcaccgtat
     2221 ggaagaaata tttgccctca gcgatgccat cactgtcttt aaagatggac gttatgtcaa
     2281 aacctttacc gatatgcagc aggttgacca cgacgcgctg gtgcaggcga tggtcgggcg
     2341 cgacattggc gatatctacg gctggcaacc tcgtagttat ggcgaggagc gcctgcgtct
     2401 tgatgctgtg aaagcactag gcgtgcgtac gccaataagt ctggcggttc gcagtggtga
     2461 aattgttggg ctgtttggtc tggtaggggc ggggcgtagc gaattaatga aaggcttgtt
     2521 tggcgggacg caaatcaccg ccggtcaggt ttatatcgac caacagccga tcgatattcg
     2581 taaaccgagc cacgccattg ccgcaggcat gatgctctgc ccggaagctc gcaaagcgga
     2641 aggcattatt cccgtgcact ccgttcgcga caatatcaac atcagtgcca gacgcaaaca
     2701 tgtgctcggc ggttgtgtaa tcaacaacag ttgggaagaa aacaatgccg atcagcacat
     2761 tcgttcgctc aacatcaaaa cgccgggcgc ggagcaactg atcatgaatc tctcaggcgg
     2821 aaatcagcaa aaagccattc tgggccgctg gttatcggaa gagatgaagg tcattttgct
     2881 ggatgaacct acgcgcggca ttgatgttgg cgctaagcac gaaatatata acgtaattta
     2941 tgcgctggcg gcgcagggcg tggcggtgct gtttgcctcc agcgacttac ctgaagtcct
     3001 cggcgttgcc gaccggattg tggtgatgcg ggaaggcgaa atcgccggtg aattgttaca
     3061 cgagcaggca gatgagcgtc aggcactgag ccttgcgatg cctaaagtca gccaggcagt
     3121 tgcctgagta aggagagtat gatgtcttct gtttctacat cggggtctgg cgcacctaag
     3181 tcgtcattca gcttcgggcg tatctgggat cagtacggca tgctggtggt gtttgcggtg
     3241 ctctttatcg cctgtgccat ttttgtccca aattttgcca ccttcattaa tatgaaaggg
     3301 ttgggcctgg caatttccat gtcggggatg gtggcgtgtg gcatgttgtt ctgccttgct
     3361 tccggtgact ttgacctttc tgtcgcctcc gtaattgcct gtgcgggtgt caccacggcg
     3421 gtggttatca acctgactga aagcctgtgg attggcgtgg cagcgggttt gttgctgggc
     3481 gttctctgtg gcctggtcaa tggctttgtt atcgccaaac tgaaaataaa tgctctgatc
     3541 acgacactgg caacgatgca gattgttcga ggtctggcgt acatcatttc agacggtaaa
     3601 gcggtcggta tcgaagatga aagcttcttt gcccttggtt acgctaactg gttcggtctg
     3661 cctgcgccaa tctggctcac cgtcgcgtgt ctgattatct ttggtttgct gctgaataaa
     3721 accacctttg gtcgtaatac cctggcgatt ggcgggaacg aagaggctgc gcgtctggcg
     3781 ggtgtaccgg ttgttcgcac caaaattatt atctttgttc tctctggcct ggtatctgcg
     3841 atagccggaa ttattctggc ttcacgtatg actagtgggc agccaatgac gtcgattggt
     3901 tatgagctta ttgttatctc cgcctgcgtt ttaggtggcg tttctctgaa aggtggcatc
     3961 ggaaaaatct catatgtggt ggcgggtatc ttaattttag gcaccgtgga aaacgccatg
     4021 aacctgctta atatttctcc tttcgcgcag tacgtggttc gcggcttaat cctgctggca
     4081 gcggtgatct tcgaccgtta caagcaaaaa gcgaagcgca ctgtctgatg cttttttcca
     4141 caacaactta acgttttttc ccaccacagc cagccgccac aacggttggc tgttcttcgt
     4201 tgcaaatggc gacctccgtc acactgtcta tacttacatg tctgtaaagc gcggtctgcg
     4261 caacacaata agaaaagaga aggaggagag ccgggtgaca gaaccgttaa ccgaaacccc
     4321 tgaactatcc gcgaaatatg cctggttttt tgatcttgat ggaacgctgg cggaaatcaa
     4381 accgcatccc gatcaggtcg tcgtgcctga caatattctg caaggactac agctactggc
     4441 aaccgcaagt gatggtgcat tggcattgat atcagggcgc tcaatggtgg agcttgacgc
     4501 actggcaaaa ccttatcgct tcccgttagc gggcgtgcat ggggcggagc gccgtgacat
     4561 caatggtaaa acacatatcg ttcatctgcc ggatgcgatt gcgcgtgata ttagcgtgca
     4621 actgcatacg gtcatcgctc agtatcccgg cgcggagctg gaggcgaaag ggatggcttt
     4681 tgcgctgcat tatcgtcagg ctccgcagca tgaagacgca ttaatgacat tagcgcaacg
     4741 tattactcag atctggccac aaatggcgtt acagcaggga aagtgtgttg tcgagatcaa
     4801 accgagaggt accagtaaag gtgaagcgat tgcagccttt atgcaggaag ctccttttat
     4861 cgggcgaacg cccgtatttc tgggcgatga tttaaccgat gaatctggct tcgcagtcgt
     4921 taaccgactg ggcggaatgt cagtaaaaat tggcacaggt gcaactcagg catcatggcg
     4981 actggcgggt gtgccggatg tctggagctg gcttgaaatg ataaccgccg cattacaaca
     5041 aaaaagagaa aataacagga gtgatgacta tgagtcgttt agtcgtagta tctaaccgga
     5101 ttgcaccacc agacgagcac gccgccagtg ccggtggcct tgccgttggc atactggggg
     5161 cactgaaagc cgcaggcgga ctgtggtttg gctggagtgg tgaaacaggg aatgaggatc
     5221 agccgctaaa aaaggtaaca ttacgtgggc ctcttttaac ctcagcgaac aggaccttga
     5281 cgaatactac aaccaattct ccaatgccgt tctctggccc gcttttcatt atcggctcga
     5341 tctggtgcaa tttcagcgtc ctgcctggga cggctatcta cgcgtaaatg cgttgctggc
     5401 agataaatta ctgccgctgt tgcaagacga tgacattatc tggatccacg attatcacct
     5461 gttgcccttt gcgcatgaat tacgcaaacg tggggtgaat aatcgcattg gttttt
//
LOCUS       DAEBXN010000197         5506 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.198, whole genome shotgun sequence.
ACCESSION   DAEBXN010000197 DAEBXN010000000
VERSION     DAEBXN010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5506
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.198"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(112..714)
                     /gene="fimB"
                     /locus_tag="KJL99_004110"
                     /pseudo
     CDS             complement(112..714)
                     /gene="fimB"
                     /locus_tag="KJL99_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
     gene            2168..2884
                     /gene="nanC"
                     /locus_tag="KJL99_004111"
     CDS             2168..2884
                     /gene="nanC"
                     /locus_tag="KJL99_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HBD4438627.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            2904..4010
                     /gene="nanM"
                     /locus_tag="KJL99_004112"
     CDS             2904..4010
                     /gene="nanM"
                     /locus_tag="KJL99_004112"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HBD4438628.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPREQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            4075..5055
                     /locus_tag="KJL99_004113"
     CDS             4075..5055
                     /locus_tag="KJL99_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HBD4438629.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
BASE COUNT         1674 a         1143 c         1158 g         1531 t
ORIGIN      
        1 ataattaaga atcacactta caataacatc aattaataaa tggttaatca tttttgatat
       61 atcgtaagaa taatgtagtt tttaacacca tccctggtat ctcaactatc tctataaaac
      121 agcatgacgc tgtcgtcctc tggctctatc ccagatgccg taaaaacgcc ctgcattgct
      181 ggcggtatac cagacagtat gacgaatatt gcgatgccca agataatcct agataagtcg
      241 cgtatctatt cccatattcg ccaaagcaaa accacacgaa tagcgtaaca tgtgcggatg
      301 aatctccagt gacaacccgg cattaccacc agaagtcgag ataatatggt aaaactgttg
      361 ccgagaaagc ggattcccct tacgtgataa aaatacccac tcgctctcag catgcggata
      421 agaagtacgg atactcaacc agttttttaa agcctgaact tctttgttca atagcgggtg
      481 cgttgttgaa aagccttttt ttaatcgatg gatatatata cactttgctt taagatcaat
      541 atccgaaatc ctcaatcgac aaatttcact cgcccggaaa ccatgaataa aacaaagcaa
      601 aatcagacaa taattacgtg ttgcatgagg cccggtattt gctgctttaa ggagtgactc
      661 gatttcacta tgggtaagga agttcctttt tttgttatca gccttattcc tcatcgtttt
      721 cccttataat tacagacgcg cactagctgt gctgggttag caataatcca acatttttat
      781 cctgattatg tttataaaag cgaacgtttg cttaataact tacgtaagtg gaccagttct
      841 tctgagcgaa cttaaatgga gtagcgatag ttacttataa taactttgcc atacaaacgc
      901 catgctcttg catgctatgt acctttatat atttatcaat ctgatacgaa tctaataata
      961 accctcatca ttaatgcaac taatcttatc tatatatcat gtgatatgtt ctgtaacaag
     1021 taaaatcaac ataaaaaatt cagcaaaatt taatatttgt taaactagcc gtctcaaata
     1081 tgacattatt agttaactgc atttgccaca aaatggctat ttcatggaac gcagtaattt
     1141 cttagtggct attatcatgc taattcttag atccctagcg attattcctg cctggttcgt
     1201 cagcttaggc catgagattg ttacattcct ttttatcctg gagatatggg ggaacgcagg
     1261 cgacgctttt tcatctacat ccgttggtaa atatagcttt ggtcactacg acttaagcct
     1321 tcctcaatca cagctattcg ccgggcgata cggcaaacaa actaagtgaa acatcatcct
     1381 gaaaacacca acatcaacaa gcctctccag atcgacatca ggagtgacca gttacaagcc
     1441 acaaaccaag aaacatattt catgatgaga attatgctca gcctgatggc gagtaaaccc
     1501 ttattttcac cactctcact gagttagctt gcaaagcttt ctttgtttag attctaaata
     1561 aattatttat aaaacgtata gttaaaagaa aatctttagt ttttgaagac caccgcaagt
     1621 gttcgtctgg cttcacatgg catcttcttc ttagaaaaag atcgacatat tttgtgacgc
     1681 gaattgcaaa tctggttttg ttgtatggat tgcgtgattt ttgatctggt ataacaggta
     1741 taaagatgca ccacgatagt caatgagact gggcatctcg taatctatgg caaacatcac
     1801 ttcagttctt tcacatcggg tgatgaaaat gcacttcagt ctgaaaggaa tatgaaaatg
     1861 agatcaacag acactctatt ttatgactct gggtaaaatg ggttgagtaa gtgatataac
     1921 ttacgaatat tcaaatcaat taaaaatcag aagagatttt atactcaggt atttaatctg
     1981 gatctctgtt tatttaaata atgttaaaag agaattttca caggagacct tatacaaaaa
     2041 atataaaata cagttatccg ttgccaaaga cactataagc ctggcaaaaa aatattacac
     2101 aacataaatg ctaattgttt atgcgggctt tgtattgctt tctgtatcct acaaatgagt
     2161 gaaatttatg aaaaaggcta aaatactttc tggcgtatta ttactgtgct tttcgtcccc
     2221 attaatttct caggctgcga cactggacgt acgtggtgga tatcgtagtg gaagccacgc
     2281 ctatgagact cgactcaaag tcagtgaggg atggcaaaat ggatggtggg caagcatgga
     2341 aagtaatacc tggaatacca ttcatgataa taaaaaggaa aatgccgcac tcaatgatgt
     2401 tcaggttgaa gttaattacg caattaaact tgatgatcaa tggacggtgc gcccgggaat
     2461 gttaacgcat tttagcagca acggcacacg ctacggaccc tacgtaaaac tgtcctggga
     2521 cgcgacaaaa gatcttaatt ttggcattcg ctatcgttac gactggaaag cttaccgaca
     2581 acaagactta tccggtgata tgtctcgtga taacgttcat cgttgggatg gatatgtcac
     2641 ttaccatatt aatagtgatt tcaccttcgc atggcaaacg acgctataca gcaaacagaa
     2701 tgattatcgc tatgcaaacc acaagaaatg ggcgacggaa aatgcctttg ttctacaata
     2761 ccatatgacg cccgatatta cgccatacat agaatatgac taccttgacc gccagggtgt
     2821 ttacaacggc agagataatt tatcggaaaa cagttatcgc attggtgtgt catttaaact
     2881 gtagtagaca ggagacagtc acaatgaata aaactataat ggcgcttgct atcatgatgg
     2941 cttcatttgc cgcaaacgcg tctgtattac cggaaactcc tgtgccattt aaaagtggta
     3001 ccggagcaat tgataacgac actgtctaca ttggtttagg tagcgcaggt acagcatggt
     3061 acaagctaga tacacaggcc aaagataaaa aatggactgc gttagctgca ttccctggtg
     3121 gaccgagaga gcaagcaacc tctgcattta ttgatggcaa tctgtatgtg tttggcggca
     3181 ttggcaaaaa cagcgaggga ttgactcagg tatttaatga cgtacacaaa tacaacccca
     3241 aaaccaatag ctgggttaaa ttgatgtcgc acgcgccgat gggcatggcg ggtcatgtga
     3301 cttttgtaca caacggcaag gcttatgtta ctggcggtgt taaccagaat atcttcaatg
     3361 gctattttga agatctcaac gaggctggaa aagattcaac cgctatagat aaaatcaacg
     3421 cccactattt tgacaaaaaa gcagaagatt atttcttcaa taagtttctg ttgtcttttg
     3481 atccctcaac acagcaatgg agttacgctg gcgaatcgcc ctggtacgga acggctggtg
     3541 cggcggttgt gaataaaggt gataaaacct ggcttattaa tggcgaagcc aaaccaggat
     3601 tgcgaacgga tgccgtattt gaacttgatt tcaccggtaa taatttaaaa tggaataagc
     3661 ttgatcccgt ctcatcacca gatggcgtcg ctggcggttt tgcggggata agcaatgatt
     3721 ctcttatatt tgctggaggg gccggattca aaggttcacg agaaaattac cagaacggta
     3781 agaactatgc gcatgaaggc ctgaaaaaat catatagcac tgatattcat ctttggcata
     3841 acgggaaatg ggataaatcg ggtgaattat cgcaaggtcg ggcctacgga gtatcattgc
     3901 cctggaataa tagtttatta attattggcg gtgaaactgc aggcggcaaa gcggtgacgg
     3961 attcagtttt aatttctgtg aaggataata aagtcacagt acaaaactaa cgcttcaggg
     4021 ccccggtaaa ggggccctgc tatcaatttg tcatttgtat tacgcgaaaa ttatatgaac
     4081 gcaataatat cacccgatta ttactatgtt cttaccgttg ctggtcagtc taatgccatg
     4141 gcgtatggcg aaggactgcc attaccggac agggaagatg cgcctcattc cagaattaaa
     4201 caattagcga gatttgcgca tacgcatccc ggaggcccgc catgtcactt taacgacatt
     4261 attccactga ctcactgccc acacgatgtt caggatatgc agggttatca ccatcctctg
     4321 gcaacgaatc atcaaacaca gtacggcacc gttggccagg cactgcatat tgcacggaaa
     4381 ttactgccct tcattcctga taatgcaggg attcttatcg ttccgtgttg ccgtggcgga
     4441 tcggctttta ccgcgggcag cgaagggaca tattcagaac ggcacggagc cagccatgat
     4501 gcttgtcgtt ggggaacgga tactccgctg taccaggatt tagtcagcag aacgcgagcc
     4561 gcactggcaa aaaatccgca gaacaaattc ctcggcgtat gctggatgca aggcgaattt
     4621 gacttaatga ccagtgacta cgcgtcacac cctcaacact ttaatcatat ggttgaagcc
     4681 tttcgtaggg atctaaaaca ataccattct cagcttaata atattactga cgcgccgtgg
     4741 ttttgcggcg ataccacctg gtactggaaa gaaaatttcc cgcattcgta tgaagctatt
     4801 tatggcaatt atcaaaataa tgttttagcc aatattattt tcgtcgactt ccagcaacaa
     4861 ggtgaaagag gactgacgaa cgcgcctgat gaagacccgg acgatttaag cacgggatat
     4921 tacggttcag cgtaccggtc accggagaac tggacgacgg cactgcgaag cagtcatttc
     4981 agcacggcag cccgtcgggg gattatttct gacagatttg tagaagcaat tttgcagttt
     5041 tggcgcgaaa ggtgagcggg gagtttattc atgggggatg tccttgttcg ttctcagagt
     5101 agacaacaaa aaacaaatga gatcaaggta atatctcttc acttgcgggc tcgaacagca
     5161 ggcaattgcc aatataataa tctggcacaa gggtgaaaaa cgcgcaccgc ataaaccgtt
     5221 gttattgctt acagattagc cggatgcctt aatagacatc cgcgcctttt cgattatggc
     5281 tcggaaatct acgaacatct acacagttta ctggaacgtt ttgggccaca gcgttcgcag
     5341 caccgtcctg atatgccgtt ctggcgatta taagtcgacg gattctggca aatagacgat
     5401 gatgatatgc ttaatgatgt tcaaagaaat taggccatta ttatattatt gcttgcattt
     5461 tctttcattc ctaaaagcac ccgccattaa tcccaggcgc attatg
//
LOCUS       DAEBXN010000198         5487 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.199, whole genome shotgun sequence.
ACCESSION   DAEBXN010000198 DAEBXN010000000
VERSION     DAEBXN010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5487
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.199"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(<1..1630)
                     /locus_tag="KJL99_004114"
     CDS             complement(<1..1630)
                     /locus_tag="KJL99_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HBD4438630.1"
                     /translation="MENSRIPREHFFTTSDNTALYYRHWPTLQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGKNSGPRGYSPSLMRSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFICRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKPW"
     gene            complement(1646..2530)
                     /locus_tag="KJL99_004115"
     CDS             complement(1646..2530)
                     /locus_tag="KJL99_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBD4438631.1"
                     /translation="MSGNTFRVVNFSKLINRFLLWRLPERKGDEVTLRIRTWWGIVIC
                     FSLVISGPRWMTLTFFTLISFLALKEYCTLISVHFSRWLYWVIPLNYLLIGFNCFELF
                     LLFIPLTGFLILATWRVFVGDPSGFQHTVSAIFWGWIMTVFALSHAAWLLMLPTINIQ
                     GGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLLGGVITTMIASLI
                     IGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLLGHGGLLDRID
                     SLIFTAPVFFYFIRYCCY"
     gene            complement(2541..3146)
                     /locus_tag="KJL99_004116"
     CDS             complement(2541..3146)
                     /locus_tag="KJL99_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBD4438632.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLTALALSLFTGL
                     LLVLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLICTAINRCRSVLLMSAER"
     gene            complement(3317..>5487)
                     /locus_tag="KJL99_004117"
     CDS             complement(3317..>5487)
                     /locus_tag="KJL99_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HBD4438633.1"
                     /translation="KQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLI
                     VLGNLTVKGNISTFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNP
                     STIKGSINARGVFINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWG
                     LINDEDALKEIFKRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEG
                     RDIATFDLAIMRTLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLG
                     YQPVVLGLLDREPLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQ
                     ELYKDAWLLPESIIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKL
                     AEMKHHGQLLRMTPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKS
                     PAYFCCRDMQVIHSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKN
                     NPKADSDAYETSADMILSELISHGEVDDQMLLNAAALIRLEDWDFLESALVSWDNLPA
                     VVLKELQQNTPRNDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDR
                     IWWSTLAKSNLTFFKFGALSNRHTPSAALAAEIDPEWWIVAMNNPRFPVDVLKARLKR
                     DPLLALELVNPELDLVRQLALNGKTRAIREQAMRKLDELY"
BASE COUNT         1582 a         1363 c         1141 g         1401 t
ORIGIN      
        1 cccacggttt accatcttta tgactggtta acaccccggc aatcagctcc agttgtgggt
       61 gccacggctg cccggtgtag atgagaatgc cacccggttc gatggcattg gctagacctg
      121 cgagtgagtt ttttacctgc tcattttcgg gaaaaagctc atataggcca gagacaatcg
      181 ccagcgtagg ccgcggagtt aacgcgctga gttcctccgg gttaaacgca tcgccctgtt
      241 caaaacgcac ccgcccagac attccccgtt gggcaatcat ctcttgcccc tgtgcaacat
      301 ttaactcact gtaatcacgt aacaaaatat cgcttacggc aggctcgttt gccagcgcat
      361 ccagtacata gcgtccatgc cctgcggcaa tgtcaaccac gcggacggct aaacctttgg
      421 cgtggagatc ggcaacggct tgtttaatca atatttgcaa atgggttttg cgctggcgaa
      481 taccgcgcca tccaacactg ttcaggtaga ttttgtcgat taaacgcccg aatgcgttac
      541 tgccttgcgg ttgattttga tagacataat ccagggaact gcctgaatca aagccggtac
      601 tcattccgag atgcaggccc gcagagtgcg caccgaacaa tttcatcgcc tttcgcataa
      661 agcgatacgc taaatcaacc ggcgataatg gcacgggtcc accagaaagg agccgccatc
      721 gatccgctga tggtcctgtg cggtcttcat gctgataatc aaatttttgc gatttgttag
      781 catataagcg gcagataaag ctttgcattt tttcaaatgc ctgcgccctg ttctcttcac
      841 ccaatgtgtc gtgataaaag cctggcagca gatgcagctc tttcagaggg ctacgtaatc
      901 tctgataaaa atcaatctgc ggttggcgat gcaccacata gtcatcacct gatatcagaa
      961 gttgcgtggg gagcgtaatc gccgccgcat cgctaacaat acgttcagac gttttataga
     1021 gatcgagcaa gatattgacg gcaatcgccc gtgtgatcag cggatcatta ttgaaactcg
     1081 ccacccgttg ccgatcgtgg gtcaaatagc gccctttcac ataggaatta ataaaaaata
     1141 gaccacgcag acgatgccat aacgccagcg caggacgtgc cagcggcaca tacaatttaa
     1201 ccttaaaggc cggagaagcc agcaccagcc cacgaattgc aggtgcataa tcatgaaccc
     1261 atgtggcaac aagcactgcg ccgacgcttt gcgcgatcac taccacctct tccagtccga
     1321 cctggctgtc gctggcagca aaacggacaa attcatcgac atcctgcact gagcgcataa
     1381 gagacgggct ataaccacgc ggccccgaat tttttccatg cccccgggca tcccaggcat
     1441 aaaaagcagt atcaggcatc gccagttcat caacgagatg ttgtagacga ccagaatgtt
     1501 catgcccgcg atgaaataag acgatgacct ttttcgcccc tggctgtaaa gtgggccagt
     1561 gccgataata caatgctgta ttatcactgg tggtaaaaaa atgttcccta gggatgcgtg
     1621 aattttccat tttctctatt ttccttcagt aacagcagta gcgaataaaa taaaaaaata
     1681 ccggagcggt gaaaattaat gagtcaatcc tgtcaaggag tccgccatgt cctagtaata
     1741 gttttccact atctttaaca ccaatatctc gtttgatggc tgacatcact acatcgccgc
     1801 aaaaaccact aataccaatt aacaaacccg ctaataatgc ctgtaatgta tttagcggtg
     1861 tcagcaacgg tccaataatc agtgacgcaa tcatggtggt gattacgccc cccagcagac
     1921 cttccagcgt ttttcccggg ctgactttgg ggaccacttt tcttctgccg caggatttcc
     1981 cccataaata ctgtgcaata tcgtttgact cggttaacgc caaaagaaat aacaccagta
     2041 acgccccgcc ctggatattt atggttggca acattaatag ccaggcggca tgactcaagg
     2101 caaaaacggt cattatccat ccccaaaaaa tggcgctcac ggtatgctga aaaccggagg
     2161 ggtcacccac aaaaactcgc caggtggcta atatcagaaa cccagtcaaa ggtataaata
     2221 acagaaacag ctcaaagcaa ttaaaaccga taagcaaata gttaagagga ataacccaat
     2281 ataaccaacg cgaaaaatgt acggatataa gcgtacaata ttctttcaat gccagaaaac
     2341 ttatcagcgt aaaaaacgtc aacgtcatcc agcgtgggcc tgaaatcacc agtgaaaaac
     2401 aaatgacgat gccccaccag gtacgaatac gtaatgtaac ttcatcccct tttctctcag
     2461 gtaaccgcca tagcagaaaa cggtttatca gcttgctaaa attaacaacg cgaaaagtgt
     2521 tgccagagat ttttccagca ttatctttca gcgctcataa gaagcacact ccgacaacga
     2581 ttaatcgcag tacagataag cagaattgaa gcaatgctcc ataaaagatt attccattgc
     2641 atccactgtg gataaatggc aacggccaga ccccacagac caaatattaa cgcgcgatca
     2701 cttttgccaa atggaccggc ataactgcga acaccgttaa tcgtctgcgc gagtaaaccg
     2761 caaaactcgg tcaatatggt gcaaaataac atgagtatca cgagtgatgc gttactttcc
     2821 ggtaaaaata aaaacggtaa atagagcgca atatcggaaa taacatcgcc agtctcattc
     2881 aaaatcgccc ccagacgtgt ttgctgattg cactcacgcg ccaacatgcc atccagcgca
     2941 ttgagcgcca tacggataaa aagcacgatg ggcaatagca aaaagaggat gggttgtgcc
     3001 accaacacca acagcaatcc ggtaaaaaga gaaagcgcca gtgccgtaag agtgatgtga
     3061 ttcgctgtaa cgtggtgctt ataaagccaa aacatcgtcg gccttaacag cgactgaaag
     3121 agcggtttta tctggtagag cgtcattatc aatccttgat aattcggttt gcgctatggt
     3181 gcatcaattc ccctgacgga gatatcgcaa cacgccgctt cattaaaaat tcagaatata
     3241 tcaaggtatt gggggctcac tgtcaggaat gattatcccc aataaatcaa gtagttaaaa
     3301 attattacgc tgttattcaa tacaactcat caagtttcct catcgcttgt tcccgtattg
     3361 cgcgcgtctt accgttaagc gccagctggc ggactaaatc cagttcaggg ttaacaagct
     3421 ctaacgccag caaaggatca cgtttcagcc tcgccttcaa tacatcaact ggaaagcggg
     3481 gattattcat cgccacaatc caccactcgg gatcaatttc tgccgccagt gccgcagagg
     3541 gggtgtggcg gttgctaagt gcgccgaact tgaaaaatgt gagattgctt ttcgccagtg
     3601 tactccacca tatacgatcc ggttgttttg ccagtacatc cagttgtgct aacgcatcgg
     3661 ggtcgagtgc ataataaaca cgtaatgctt catcaacctg cgcacgggag ctattttcct
     3721 gcctcagaaa aaacttcgcc caaatatcat tgcgtggcgt gttttgctgt aattccttga
     3781 gaacaacagc gggtaaatta tcccaactaa ctaatgcact ctctaaaaag tcccagtctt
     3841 ccaaacgtat cagggcggcg gcatttaata gcatctggtc atcaacttcg ccgtggctaa
     3901 tcagctcaga caaaatcata tcagcgctgg tttcatatgc atctgaatct gctttcggat
     3961 tatttttccg atgcgataaa gcacgttgcc aaattttttg tttcatcgac gtgctcagat
     4021 actgattttt cgccagatta ctccacccct caggatcgtt gaaacgtgtc aataaccgtt
     4081 gtaaagcagc gtcagagtga attacctgca tatcccgaca gcaaaagtag gcaggggatt
     4141 ttttctccat ccttttaaca aaattaaact gtaagttatc tggcaatgct aaaaataaca
     4201 cacgtattag atcatcgtca ttctcgaatt tttggtataa ataggctgca actttgtcac
     4261 tctcttgtgg cgtcatccgc aataattgac catggtgttt catctctgcc agcttgaacg
     4321 ctaagcttgt gacaacattg tttgatgaat cgttcgccaa ctgcattgcc tgattgtaag
     4381 tgagttgtcc tccagcaccg acctgacgaa tagaggaatc atcactgcgg atatagccat
     4441 caataatgga ttcgggcaat aaccacgcat ctttataaag ctcctgaatg atcatatccc
     4501 aatctctttt cgccagagtc atgacgatat ccagagggag attttcacgt agcgcaagat
     4561 tcaacggacc gacaccatca ggtagacgac tcattcgcgt cattatttcg acagagagcg
     4621 gttcacggtc gagcaacccc agaacaacag gctggtaacc caattgctga tcggtcaact
     4681 ccagaatctc attgtcaggc atatatctcg ccatactttc aatcgtttgt tcatcattgc
     4741 tattaataag ttgtttgcgt aggttagctg aaattccttt aagccttggg agagttcgca
     4801 ttatggcaag atcaaatgtc gcaatatctc gtccttcaaa tgcagcaacg ttgccttcat
     4861 ccagcatttg caattcctgc actaactcag gagaaggctt agggcgaaac agcgcttctt
     4921 ttttcatctg attacagaca ttccctgcca tgcgaatatt gcttcgctta aaaatttctt
     4981 tcagagcatc ttcatcattt attagacccc aataatatgc atcaggattt attatcatca
     5041 gcgctttttt gacattttca gtgtcatttt tgtcactcgc acggaccatg aattcactat
     5101 ttgtcgaaga cgcaacaaca ggtgcaatta tgtcattgat aaatacccca cgcgcattaa
     5161 tactcccctt aatcgtagac ggattatcgt aatatgagtc gataaatacc agtccacttg
     5221 catttatcga accggtaatt aacagtggtg aatccgtaac tatattcgtt gccgtcacgt
     5281 taccgagaat aacccatgga ttagaaagag agaaagtgga aatattacct ttgactgtca
     5341 aattgccaag aacaatcaat ttttgattac tctctatatt accttctacc actaaatcgc
     5401 cacgatgcac tttccagtcc gatgcagata aaccgaaggg gacaagattc acgctactgt
     5461 tgatagcttg ttcaacttgt tgtttat
//
LOCUS       DAEBXN010000199         5414 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.200, whole genome shotgun sequence.
ACCESSION   DAEBXN010000199 DAEBXN010000000
VERSION     DAEBXN010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5414
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.200"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            470..1660
                     /locus_tag="KJL99_004118"
     CDS             470..1660
                     /locus_tag="KJL99_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl9 family transposase"
                     /protein_id="HBD4438634.1"
                     /translation="MPARKVCQTFFRNALASTHQYRQNAIIDSAAALVGGASLSLTSI
                     GRHLPGPARVKDKIKRVDCLLGNKRLHNDIPLIFKNITSMMTNKLSWCVIAVDWSGYP
                     FQEYHVLRASLLCDGRSIPLMSQVFPSKKNNEAVEIAFLDALSGAISPRTRVVIVTDA
                     GFQSAWFRHIKSQGWDFIGRIRGVVKFRLDSDKDKWLDIKMCRGSSEAKYLGTGTLAR
                     KKRSQCEGHFYLYKHSPKGRKSRRARGRPGLPTTEKEQKAAGREPWLIFSNTAEFNAK
                     KIMKLYSRRMQIEQNFRDEKSERFGFGLRASRSRRGERFLVLSLLVTLASIVLWLLGY
                     HFENKGFHLKYQANSLKKRRVLSFLTLAENVLRFNPELLRRAQPEKILCRLASTYRSM
                     VLAY"
     gene            complement(2050..3510)
                     /gene="gtrII"
                     /locus_tag="KJL99_004119"
     CDS             complement(2050..3510)
                     /gene="gtrII"
                     /locus_tag="KJL99_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase GtrII"
                     /protein_id="HBD4438635.1"
                     /translation="MIKINLFKNANLLAFISCFAISIYCYWGWLYDGTLNIDGEFTNN
                     FYQTITLGRWFHTFLRHYFLPEPFSLYITPLIALSFIIISAFIICRSLKLESYELLIG
                     MLVFITFPQISYQLEFLNQADTVGIAFLLAAISAIIFHSQKNRIVIFSGIVLSILSMA
                     IYQTFVTYIIAFVIGLQINSIIRNEKNIRESFYSSCLSLSLIALSTLIYLLLTKAIKH
                     YFSLESNEYISNYIQNASDIKWLVKSAIDNIYNFYNNPPTGLNLYKWLLIPLLILMFT
                     LTYKLKTRSIYLISSIIFIYILPVIFIVVVGSGAPPRLFVLMPIVAVILFSCLSNFRS
                     IKYLNCMFFLFIIFNGVSTSKNLFLNDTLARQKDISLAKEISYTSQTKGISLNGKYIY
                     IYGSNDSGNMLSMSADTFGKSFFWWDGGNYFRMVAFMNYYGICNCKPANKEQIEKIYP
                     IVKSLPSWPNPDSIAEINGLVIIKLSEKKGWLPFNI"
     gene            complement(3507..4436)
                     /locus_tag="KJL99_004120"
     CDS             complement(3507..4436)
                     /locus_tag="KJL99_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBD4438636.1"
                     /translation="MKISLVVPVFNEEEAIPVFYKTVREFQELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHASGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGADMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGPTDVVEYVRAERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWTYIGLFVASISFLYGAWMIIDTLVFGNPVRGYPSLLVSILFLGGVQL
                     IGIGVLGEYIGRIYIEVKNRPKYIIKKSHRGNP"
     gene            complement(4433..4795)
                     /locus_tag="KJL99_004121"
     CDS             complement(4433..4795)
                     /locus_tag="KJL99_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HBD4438637.1"
                     /translation="MLKLFVKYTSIGVLNTLIHWVVFGVCIYAAHTSQALANFTGFVV
                     AVSFSFFANARFTFKALTTAMRYMLYVGFMGILSVIVGWAADKCSLPPIVTLITFSAI
                     SLVCGFVYSKFIVFRDAK"
     gene            complement(5057..>5414)
                     /locus_tag="KJL99_004122"
     CDS             complement(5057..>5414)
                     /locus_tag="KJL99_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016042215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBD4438638.1"
                     /translation="VFVHTKPAYRSDGTKTAAVRKMRTDSNKAWKGALKRAGISNFRF
                     HDLRHTWASWLVQSGVSLLALKEMGGWETLEMVQRYAHLSAGHLTEHASKIDAIISRN
                     GTNTAQEENVVYLNAR"
BASE COUNT         1635 a         1071 c         1106 g         1602 t
ORIGIN      
        1 cgattttgtt attatacatt taaagtaggc tctccagttt tgtgtgattt ttaaacactc
       61 attgcaaata tgaatgcctg aaagtttatg attattaatc aacgagttca atattaattt
      121 attatttttc aaagtcgcac tatataggca tttacttccg cagtaccacc gatagtatcg
      181 atatcaaatc agcctgcggc cgcgataact ttcgtaacat tctgccagcg gctttgttga
      241 ataaatcagg ttcaagataa tcgtctctgc atcaggttag agtttcaggc aatacatcct
      301 ctttctggca tacctgccac tctcgcacca tggtcatagc ctttacgatc tgcatactca
      361 ccagaccaga gcccgctccc ttttaggctg aggaatcccc agaaaagaag acaggcatag
      421 agttttgtga tctactgatt gcgctaacaa attcaatgag atcacctcta tgcctgcccg
      481 taaagtatgc cagacttttt tccgtaatgc tttagcttcc acgcatcagt atcgacaaaa
      541 tgccattatt gattctgctg ctgctttagt cggtggcgca tctctttctc ttaccagcat
      601 tggccggcat ttacccggtc ctgctcgcgt gaaagataaa ataaaacgcg ttgactgtct
      661 gttgggtaat aaacgacttc ataatgatat ccctctgata ttcaaaaata ttacatccat
      721 gatgacaaat aaactgtcgt ggtgtgttat cgctgttgac tggagcggtt acccttttca
      781 ggaatatcat gtccttcgtg ccagcctcct ctgcgacggt cgctctatcc ccctgatgag
      841 tcaggtcttc ccgtcaaaaa aaaataacga ggcggtggaa atcgcttttc tggatgcgct
      901 ttccggggcc atttctcccc ggactcgtgt tgttattgtt actgatgctg gttttcaaag
      961 tgcatggttc cgtcatatca aatcacaggg atgggatttt attggtcgta tcaggggggt
     1021 agtgaagttc aggctggata gtgacaaaga taagtggctg gacataaaaa tgtgcagggg
     1081 gagttcagag gcaaaatacc tggggacggg aaccctggcc cggaaaaaac gctcgcagtg
     1141 tgaaggacat ttttatcttt ataaacattc acctaaaggc agaaaaagca ggcgtgccag
     1201 agggaggccg ggcttaccaa caacggaaaa agaacaaaaa gccgcaggca gagaaccatg
     1261 gttaatattc agcaatacag ctgagtttaa tgccaaaaaa atcatgaaac tgtacagtcg
     1321 aagaatgcag atagagcaaa actttcggga tgaaaaaagt gaacgctttg gttttggcct
     1381 tcgggcgagc agaagtcgga ggggagagcg ttttctggta ttaagtctac tggttacact
     1441 ggccagtatc gtactctggc tacttggtta tcattttgaa aataaaggat ttcatttaaa
     1501 gtatcaggct aatagtctca aaaaacgaag agtattgtct tttctgacgc tggctgaaaa
     1561 cgtcctgaga tttaaccctg aacttctacg acgagcacaa cctgaaaaga tattgtgccg
     1621 gttggccagc acttaccgaa gtatggtgct ggcttactga tgaaaatttt ctggggatcc
     1681 ctcagccttt cggggaggaa tgagggctct gatattcttt tgccgtagtt catcgtgaaa
     1741 ccgtcggatg tcatttggcc tgagtgaccc atgcctccca aaagcagagt cgctagtgtt
     1801 atgtaattag gcggttaaac ttcaattctc agtgttaagt ttgggtaaac gcttgtaaat
     1861 ggtattgcta attttttaat tgactaatat tagaaacggt tagtgtgatt gtatatagat
     1921 atttgagtgt aaggtattta aaggggcatt cagcccctcc gtctatttgt gattgcatcc
     1981 attgatgtgt gtattatttt gagcttgaaa aagggaggcg ctctttaata ataaataaat
     2041 ctatcaaagc taaatattaa atggaagcca cccttttttc tctgatagtt ttattatcac
     2101 caaaccattt atttcagcta tcgaatctgg attcggccag gaaggtaaac tcttcacaat
     2161 tggataaatc ttctctattt gttctttatt tgctggtttg caattacaga ttccatagta
     2221 attcataaat gcaaccatcc taaaatagtt gccaccatcc caccaaaaaa aagattttcc
     2281 aaaagtgtct gcgctcatgg agagcatatt tcctgagtcg tttgaaccat atatatatat
     2341 atattttccg ttaagggaaa tgccttttgt ttgagatgtg tatgatattt ctttagctaa
     2401 agagatatct ttctgtcttg cgagagtatc attcaaaaat agatttttgg atgttgaaac
     2461 gccattaaat ataataaata aaaaaaacat gcagtttagg tattttatag agcgaaaatt
     2521 gcttaagcaa gaaaacaaaa ttactgctac tataggcatt aaaacaaaca ggcgaggtgg
     2581 cgcccctgag ccaacaacaa cgataaatat aaccggaagt atataaatga aaatgattga
     2641 tgaaatcaaa taaattgatc ttgtttttaa tttatatgtt agggtaaaca tcagaattaa
     2701 taaaggaatc agtaaccact tgtataggtt taaaccagtg ggaggattgt tatagaagtt
     2761 atatatatta tctatggctg atttaacaag ccatttaata tcgcttgcat tttgtatata
     2821 atttgagatg tactcgttcg attcaagcga aaaataatgc ttgatagctt tggttaatag
     2881 caggtaaatt aaggtagata aagctatgag ggatagagat aaacatgaac tataaaaaga
     2941 ttcacgaata tttttctcat ttcgtattat cgaatttatc tgcaacccaa tgacgaatgc
     3001 aataatatat gttacgaatg tttggtagat tgccattgaa agaatggaca gtactatacc
     3061 agaaaatatc acaatcctat ttttttgcga gtgaaaaata attgctgata tcgctgccag
     3121 tagaaaagca attcccacag tatcagcttg gttaagaaac tctaattgat aggagatctg
     3181 agggaaggta ataaatacta acatacctat caataattca taagattcta gctttagcga
     3241 tctgcagatt ataaatgctg aaatgatgat aaaagataag gctattaatg gtgttatata
     3301 aagtgaaaaa ggctcaggga gaaagtaatg tcgcaaaaaa gtgtgaaacc accgcccaag
     3361 cgtgattgtt tgataaaaat tatttgtaaa ctcgccatca atattaagtg ttccatcgta
     3421 caaccacccc caataacaat aaattgatat tgcaaaacat gatatgaagg caaggagatt
     3481 tgcattttta aataaattaa ttttaatcat gggttacctc gatgagattt ttttatgatg
     3541 tatttgggtc tattttttac ttcaatatag attctaccta tatattctcc gagaacacca
     3601 atcccgatca gttgcactcc acccaagaaa agtattgata caagcaggga gggatacccg
     3661 cgtactgggt taccaaagac aagggtgtct ataatcatcc atgcaccata taaaaatgaa
     3721 atgcttgcaa caaacaagcc tatataagtc catacgcgaa gaggaaaggt tgaaaaactt
     3781 gtgatacctt ccagtgccag attccataat ttccagccat taaattttga gatgccagca
     3841 acacgctctg cgcgtacata ttcaacgaca tccgtcggac cacccaccca gctcagtatg
     3901 cctttcatga aaagattgcg ctcaggcaac agtttaatgt tctccacaac ctcacgagac
     3961 atgagtcgaa aatctccgac attttcctcg atctttgggg tgcttatttt gttgtgtaat
     4021 ttatagaacc actcagctgt cttacgtttc agtcgtccat cagttgagcg gtctgaacgt
     4081 ttagcaagca ccatgtcagc acctgcctgc cacttttcaa taagatgagg gataacctca
     4141 attgggtctt gcaggtcgac atcaatagga attacagcat cgccgcttgc atggtctaac
     4201 cctgcaaata aggctggttc tttaccaaag ttgcgtgtaa atgacagcgg aacaactagc
     4261 gggtctgaaa cagccagcgc gttaataatt gactctgtgg catctttact tccgtcattt
     4321 atgaatacaa tttctacttc atatggcttc aactcttgga attcacgtac cgttttatag
     4381 aaaacaggta tcgcttcttc ttcattgaag acaggaacga caagagatat tttcatttcg
     4441 catccctaaa gacaatgaac tttgaataga cgaaaccgca caccagactg atggcggaga
     4501 aggtgataag agtgactatt ggaggaagtg agcatttatc agctgcccaa ccaacaatca
     4561 cactcaatat tcccataaat cccacgtata acatgtagcg catcgctgta gtcaatgctt
     4621 tgaatgtgaa tcttgcattc gcgaagaagc taaagctcac agccactacg aaacctgtga
     4681 agtttgccag agcctgactg gtatgtgcgg catagataca tacaccaaaa accacccagt
     4741 gtataagtgt gttcagcaca cctatagatg tgtactttac aaataacttt aacatttatt
     4801 taatcaatga gctctgaaag gcatgaagtc tatcatccaa gtctcaattg atcgatactt
     4861 gctatgtctg atgagacaaa actgagacac ataaggcctc acaatggctt gcaaggcttt
     4921 acatgttttg atgtggtggg acgtgtgagc gcagtgttga tgggataatc ctttgaatta
     4981 caagcggatt cttataattc gtaatgcgaa ggtcgtaggt tcgactccta ttatcggcac
     5041 catttaaatc aataagttac ctcgcattta agtaaactac gttctcctct tgtgccgtat
     5101 ttgtgccatt gcgacttata atcgcatcga ttttgctcgc gtgctcggta agatgcccgg
     5161 ctgaaaggtg ggcgtatctt tgaaccattt cgagagtttc ccatcctccc atctctttaa
     5221 gcgcaagaag agagacaccg gactgaacca gccagcttgc ccaggtatgc ctcaggtcat
     5281 ggaagcggaa gttgctaatg cctgcccgct ttaacgctcc cttccatgcc ttgttgctgt
     5341 cggttctcat cttccttacc gctgctgttt ttgttccgtc gcttcggtag gcaggtttgg
     5401 tgtggacaaa cacc
//
LOCUS       DAEBXN010000200         5400 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.201, whole genome shotgun sequence.
ACCESSION   DAEBXN010000200 DAEBXN010000000
VERSION     DAEBXN010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5400
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.201"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..497
                     /locus_tag="KJL99_004123"
     CDS             <1..497
                     /locus_tag="KJL99_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="COX aromatic rich motif-containing protein"
                     /protein_id="HBD4438639.1"
                     /translation="EIAFPANTPVYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHL
                     IANEPGTYDGISASYSGPGFSGMKFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEK
                     LAAPSEYNQVEYFSNVKPDLFADVINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHA
                     ESAH"
     gene            519..2510
                     /gene="cyoB"
                     /locus_tag="KJL99_004124"
                     /pseudo
     CDS             519..2510
                     /gene="cyoB"
                     /locus_tag="KJL99_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
     gene            2500..3114
                     /locus_tag="KJL99_004125"
     CDS             2500..3114
                     /locus_tag="KJL99_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HBD4438640.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            3114..3443
                     /locus_tag="KJL99_004126"
     CDS             3114..3443
                     /locus_tag="KJL99_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HBD4438641.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            3455..4345
                     /gene="cyoE"
                     /locus_tag="KJL99_004127"
     CDS             3455..4345
                     /gene="cyoE"
                     /locus_tag="KJL99_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HBD4438642.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPSVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            4529..4840
                     /locus_tag="KJL99_004128"
     CDS             4529..4840
                     /locus_tag="KJL99_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HBD4438643.1"
                     /translation="MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            4824..4982
                     /locus_tag="KJL99_004129"
     CDS             4824..4982
                     /locus_tag="KJL99_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005053055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HBD4438644.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAT
                     RIAKEKCA"
     gene            4975..5373
                     /locus_tag="KJL99_004130"
     CDS             4975..5373
                     /locus_tag="KJL99_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBD4438645.1"
                     /translation="MRNSDCAAKALIDRQSGELIGICTFTAYSLERQRVSGVLQGSQP
                     SEIGVVRLVMLGGARKYQKRGFGQDLLCDFFEHVKKIHPALPIKGVYLDADPAAINFY
                     ARLGFVQLSATPNAFGAVPMFLAIQHILAA"
BASE COUNT         1178 a         1344 c         1426 g         1452 t
ORIGIN      
        1 atgaaatcgc tttcccggcg aacactccgg tgtacttcaa agtgacctcc aactccgtga
       61 tgaactcctt cttcattccg cgtctgggta gccagattta tgccatggcc ggtatgcaga
      121 ctcgcctgca tctgatcgcc aacgaacccg gtacttatga cggtatctcc gccagctaca
      181 gcgggccggg cttctcaggc atgaagttca aagctattgc aacaccggat cgcgccgaat
      241 tcgaccaatg ggtcgcaaaa gcgaaacagt cgccgaacac catgtctgac atggctgcgt
      301 tcgaaaaact ggccgcgcct agcgaataca accaggtgga atatttctcc aacgtgaaac
      361 cagacttgtt tgccgatgtg attaacaagt ttatggctca cggtaagagc atggacatga
      421 cccagccaga aggtgagcac agtgcacacg aaggtatgga aggcatggac atgagccacg
      481 cggaatccgc ccattaaagg ggttgaggaa gaataaagat gttcggaaaa ttatcacttg
      541 atgcagtccc gttccatgaa cctatcgtca tggttacgat cgctggcatt attttgggag
      601 gtctggcgct cgttggcctg atcacttact tcggtaagtg gacctacctg tggaaagagt
      661 ggctgacctc cgtcgaccat aaacgcctcg gtatcatgta tatcatcgtg gcgattgtga
      721 tgttgctgcg tggttttgct gacgccatta tgatgcgtag ccagcaggct cttgcctcgg
      781 cgggtgaagc gggcttcctg ccacctcacc actacgatca gatcttcacc gcgcacggcg
      841 tgattatgat cttcttcgtg gcgatgcctt tcgttatcgg tctgatgaac ctggtggttc
      901 cgctgcagat cggcgcgcgt gacgttgcgt tcccgttcct caacaactta agcttctggt
      961 ttactgttgt tggcgtgatt ctggttaacg tttctctcgg cgtgggcgaa tttgcgcaga
     1021 ccggctggct ggcctatcca ccgctatcgg gaatagagta cagtccggga gtcggtgttg
     1081 attactggat atggagtctc cagctatccg gtataggtac gacgcttacc ggtatcaact
     1141 tcttcgttac cattctgaag atgcgcgcac cgggcatgac catgttcaag ataccagtat
     1201 ttacctaggc atcactgtgc gcgaacgtac tgattattgc ttccttccca attctgacgg
     1261 ttaccgtcgc gttgttgacc ctggatcgct atctgggcac ccatttcttt accaacgata
     1321 tgggtggcaa catgatgatg tacatcaacc tgatttgggc ctggggccac ccggaagttt
     1381 acatcctgat cctgcctgtt ttcggtgtgt tctccgaaat tgcggcaacc ttctcgcgta
     1441 aacgtctgtt tggttatacc tcgctggtat gggcaaccgt ctgcatcacc gtgctgtcgt
     1501 tcatcgtttg gctgcaccac ttctttacga tgggtgcggg cgcgaacgta aacgccttct
     1561 ttggtatcac cacaatgatt atcgccatcc cgaccggggt gaagatcttc aactggctgt
     1621 tcaccatgta tcagggccgc atcgtgttcc attctgcgat gctgtggact atcggtttta
     1681 tcgtcacctt ctcggtgggc ggtatgactg gcgtgctgct ggcagtaccg ggcgcggact
     1741 tcgttctgca taacagcctg ttcctgattg cgcacttcca taacgtgatc atcggcggcg
     1801 tggtcttcgg ctgcttcgca gggatgacct actggtggcc taaagcgttc ggtttcaaac
     1861 tgaacgaaac ctggggtaaa cgcgcgttct ggttctggat catcggcttc ttcgttgcct
     1921 ttatgccgct gtatgcgctg ggcttcatgg gcatgacccg tcgtttgagc cagcagattg
     1981 acccgcagtt ccacaccatg ctgatgattg cagccagcgg tgcagtactg attgcgctgg
     2041 gtattctctg cctcgttatt cagatgtacg tttctattcg cgaccgcgac cagaaccgtg
     2101 acctgactgg cgacccgtgg ggtggccgta cgctggagtg ggcaacctct tccccgcctc
     2161 cgttctataa ctttgccgta gtgccgcacg ttcacgaacg tgatgcattc tgggaaatga
     2221 aagagaaagg cgaagcgtat aaaaagcctg accactatga agaaattcat atgccgaaaa
     2281 acagcggtgc aggtatcgtc attgcagctt tctccaccat cttcggtttc gccatgatct
     2341 ggcatatctg gtggctggcg attgttggct tcgcaggcat gatcatcacc tggatcgtga
     2401 aaagcttcga cgaggacgtg gattactacg tgccggtggc agaaatcgaa aaactggaaa
     2461 accagcattt cgatgagatt actaaggcag ggctgaaaaa tggcaactga tactttgacg
     2521 cacgcgactg cccacgcgca cgaacacggg caccacgatg caggcggaac caaaatcttc
     2581 ggattttgga tctacctgat gagcgactgc attctgttct caatcttgtt tgctacctat
     2641 gccgttctgg tgaacggcac cgcaggcggc ccgacaggta aggacatttt cgaactgccg
     2701 ttcgttctgg ttgaaacttt cttgctgttg ttcagctcca tcacctacgg catggcggct
     2761 atcgccatgt acaaaaacaa caaaagccag gttatctcct ggctggcgtt gacctggttg
     2821 tttggtgccg gatttatcgg gatggaaatc tatgaattcc atcacctgat tgttaacggc
     2881 atggggccgg atcgcagcgg cttcctgtca gcgttctttg cgctggtcgg cacgcacggt
     2941 ctgcacgtca cttctggtct tatctggatg gcggtgctga tggtgcaaat cgcccgtcgc
     3001 ggcctgacca gcactaaccg tacccgcatc atgtgcctga gcctgttctg gcacttcctg
     3061 gatgtggttt ggatctgtgt gttcactgtt gtttatctga tgggggcgat gtaatgagtc
     3121 attctaccga tcacagcggc gcgtcccatg gcagcgtaaa aacctacatg acaggcttta
     3181 tcctgtcgat cattctgacg gtgattccgt tctggatggt gatgacaggg gctgcctctc
     3241 cggccgtaat tctgggaaca atcctggcaa tggcagtggt acaggttctg gtgcatctgg
     3301 tgtgcttcct gcacatgaat accaaatcag atgaaggctg gaacatgacg gcgtttgtct
     3361 tcaccgtgct aatcatcgct atcctggttg taggctccat ctggattatg tggaacctca
     3421 actacaacat gatgatgcac taagagcggc ggttatgatg tttaagcaat acctgcaagt
     3481 aacgaaacca ggcatcatct ttggcaacct gatctcggtg attgggggat tcctgctggc
     3541 ctcaaagggc agcattgatt atcccctgtt tatctacacg ctggttgggg tgtcactggt
     3601 tgtggcgtcg ggttgtgtgt ttaacaacta catcgacagg gatatcgaca gaaagatgga
     3661 aaggacgaag aatcgggtgc tggttaaagg cctgatctct cctgctgtct cgctggtgta
     3721 cgccaccttg ctgggtattg ctggctttat gctgctgtgg tttggcgcga atccgctggc
     3781 ctgctggctg ggggtgatgg gctttgtggt ttatgtcggc gtctatagcc tgtacatgaa
     3841 acgccactca gtctacggca cattgattgg ttcgctctcc ggcgctgcgc cgtcggtgat
     3901 tggctactgt gcagtaaccg gcgagtttga tagcggcgca gcgatcctgc tggctatctt
     3961 cagcctgtgg cagatgcctc actcctatgc catcgccatt ttccgcttta aggattacca
     4021 ggcggcaaac attccggtat tgccagtggt aaaaggcatt tcggtggcga agaatcacat
     4081 cacgctgtat atcatcgcct ttgccgttgc cacgctgatg ctctctcttg gcggttacgc
     4141 tgggtataaa tatctggtgg tcgccgcggc ggttagcgtc tggtggttag gtatggctct
     4201 gcgcggttat aaagttgctg atgacagaat ctgggcgcgc aagctgttcg gcttctctat
     4261 catcgccatc actgccctct cggtgatgat gtccgttgat tttatggtac cggactcgca
     4321 tacgctgctg gctgctgtgt ggtaacaaaa cctctctatt aaaaaggtgc tacggcacct
     4381 ttttattgtc tgagatcaga gatacggcta ataaccgtat ctcttgacag agtgagcaga
     4441 tgcacataaa ttaatcatac ggttgatagc cgtattatgt atgagatcga ctccctacga
     4501 tttcattcag ttaatcaaag aagtcataat gcctggaaaa acagccgctc tatacgatgt
     4561 cgataaaacg ctgaaaaatg cgcgggtcga actcaaaact agtcccgacg ccaaaaacaa
     4621 acttcgtgaa gctgcgcaag ccgttggggt agatttaagc gcttttattt tgagcgctgc
     4681 tatggaacgc gccgaaagtg tgcttgataa ccaacgtcgc cgtgagcttt cgaatcaaag
     4741 ctgggaactg atgaaccaac tcatcgctga acctgctcaa ccgacgctcg ccctcaaggc
     4801 gttaatgaaa aggaaaaaca gcgatggtcg acaagcatga agagattact ctgcccatag
     4861 tcctctcctg taattatcag tctgatatta cttatcctgg gcaaaaacag tttgattgcg
     4921 gtaaccctgt tatcgataaa tttgtacgcg ctacgcgcat cgctaaagaa aagtgtgcgt
     4981 aatagcgact gtgcggctaa agcacttatt gacagacaaa gtggtgaact gattggcatc
     5041 tgtactttta cggcatattc gctggaaaga caacgcgttt ctggcgtcct tcagggttca
     5101 caaccttcag aaattggtgt tgtcagatta gtcatgttgg ggggagcgcg gaagtatcaa
     5161 aagcggggct ttggtcagga cctactatgt gatttttttg aacatgtaaa aaaaattcac
     5221 ccggcattac caattaaagg ggtttatctt gatgctgacc ctgccgccat taacttttat
     5281 gctcgtctcg gctttgttca gctttcagcg acaccaaatg cttttggtgc tgtacctatg
     5341 tttttggcga ttcagcatat tctcgcggct tagattatta tcgccacccg ccagggcaag
//
LOCUS       DAEBXN010000201         5357 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.202, whole genome shotgun sequence.
ACCESSION   DAEBXN010000201 DAEBXN010000000
VERSION     DAEBXN010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5357
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.202"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..171
                     /locus_tag="KJL99_004131"
     CDS             <1..171
                     /locus_tag="KJL99_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBD4438646.1"
                     /translation="IRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTP
                     KRYRKKLTEFNQ"
     gene            276..1628
                     /gene="celF"
                     /locus_tag="KJL99_004132"
                     /gene_synonym="chbF"
                     /pseudo
     CDS             276..1628
                     /gene="celF"
                     /locus_tag="KJL99_004132"
                     /gene_synonym="chbF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707378.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
     gene            1641..2399
                     /gene="chbG"
                     /locus_tag="KJL99_004133"
     CDS             1641..2399
                     /gene="chbG"
                     /locus_tag="KJL99_004133"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HBD4438647.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDPSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(2446..4707)
                     /gene="katE"
                     /locus_tag="KJL99_004134"
     CDS             complement(2446..4707)
                     /gene="katE"
                     /locus_tag="KJL99_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HBD4438648.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKAMK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSTDGSFMDELLTLMAEHR
                     VWSRIPKIDKIPA"
     gene            4890..5153
                     /gene="cedA"
                     /locus_tag="KJL99_004135"
     CDS             4890..5153
                     /gene="cedA"
                     /locus_tag="KJL99_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HBD4438649.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
BASE COUNT         1322 a         1188 c         1402 g         1445 t
ORIGIN      
        1 atccgtatta attttgcaaa aaaacaactg gaaatgacca actattccgt gacggatatt
       61 gcgtttgagg ccggttatag tagcccgagt ttgtttatta aaacgtttaa gaaattaacg
      121 tcctttacgc ctaagagata tcgtaagaaa ttgactgaat ttaatcagta agtcgttata
      181 cctgacaatt cacatatctg tcctgttgct ggattattta tgtccggggg ggcagatatg
      241 ccagatatca gtattctgta ctgaagggag aaattatgag ccagaaatta aaagtcgtca
      301 ctattggtgg cgggagcagc tataccccgg agttactgga aggatttatt aagcgttatc
      361 acgaattgcc ggtcagcgaa ttatggctgg tggatgtcga gggtggcaaa gctaaactgg
      421 atattatttt cgatctctgc caacggatga ttgataacgc tggcgtcccg atgaagcttt
      481 ataaaacgct ggattgccgc gaagcattga aagatgctga tttcgttact acccaactgc
      541 gcgttggcca attaccggcg cgtgaactgg atgaacgtat tccattaagt catggttatc
      601 ttggtcagga aaccaacggc gcgggcggtt tgtttaaagg tctgcgtacc attccggtga
      661 tttttgacat cgtaaaagat gtcgaagaac tttgtccgaa tgcatgggtg attaacttca
      721 ctaacccggc gggaatggtt actgaagccg tttatcgtca taccggattt aaacgcttta
      781 tcggcgtgtg taatattccg atcggcatga agatgtttat tcacgatgtt ctgatgctga
      841 aagacagcga tgatttatct atcgatttgt ttggcctcaa ccatatggtg ttcattaagg
      901 atgtgctggt aaatggcaag tcgcgctttg ccgaattgct tgatggtgtg gcatccgggc
      961 agttaaaagt atctggcgtt aaaaatattt tcgatctgcc atttagcgaa ggcttaattc
     1021 gttctctgaa tctgttgccg tgttcttatt tgctttatta cttcaagcaa aaagagatgc
     1081 tggctattga aatgggcgaa tactacaaag gcggcgcatg agcgcaggta gtacagaaag
     1141 tcgagaaaca actttttgag ctgtataaaa acccggagtt gaaagttaag ccgaaagaac
     1201 tggaacagcg cggtggggct tattactctg atgcagcgtg cgaagtgatc aacgctatct
     1261 acaacgacaa gcaagctgaa cattacgtta atatcccgca tcatgggcat attgataata
     1321 ttccggcaga ctgggcggta gaaatgacct gtaagctggg gcgcgatggc gcgacgccac
     1381 atccgcgcat tacgcatttc gatgataaag tgatggggct gattcacacc attaaaggct
     1441 tcgagattgc tgccagcaac gccgcactta gcggagaatt taacgatgtg ttactggcgc
     1501 taaaccttag tccgttggtg cattccgatc gcgatgctga gctgctggca cgcgagatga
     1561 ttctggcgca cgagaaatgg ctgccaaatt ttgccgactg catcgcagag cttaaaaaag
     1621 cacattaacc gaggctgatt atggaacgct tactgattgt taatgccgat gattttggct
     1681 taagcaaagg tcagaactac ggcattatcg aggcctgtcg caatgggatt gtcacatcga
     1741 cgacggcgct ggtgaatggg caggctattg accatgcggt gcagttgagt cgtgatgaac
     1801 cgagtctggc catagggatg cactttgtcc ttactatggg caagccactg acagctatgc
     1861 cggggttaac ccgcgatggt gtgctgggaa aatggatctg gcagttggca gaagaagatg
     1921 ctttaccgct ggaagaaatt actcaggagc ttgccagtca gtatttgcgt ttcattgagc
     1981 tatttggacg caaacctacg catcttgata gccatcatca tgtgcatatg ttcccgcaga
     2041 ttttcccgat tgttgccagg tttgcggctg aagaggggat tgcgttgcgc atcgaccgtc
     2101 agccgctatc taacgctggt gatttaccgg ctaatctgcg cagttcgcag ggattcagta
     2161 gcgcgtttta tggtgaagag attagcgagg cactgttcct gcaagtgctg gatgacgcaa
     2221 gccatcgggg tgatccttcg ctggaggtga tgtgtcatcc ggcgtttatc gataatacta
     2281 tccgtcagag tgcttactgt ttcccacggt taacggagct ggaagtgctg acatcagcgt
     2341 cgttgaaata tgcaattgct gagcgtggtt atcggttggg tagttatctt gatgtgtaag
     2401 taaggtggta aggtcactcc ccaggggacg tgaccttatc catatttacg ccggaatttt
     2461 gtcaatctta ggaatgcgtg accacacgcg gtgttctgcc atcagcgtta acagttcatc
     2521 cataaaacta ccgtcggtgc tgtcagcttc cacaatccct tcttcaccct ggtcagctac
     2581 cttgattgtt gctttaaact tgcgcgcgtc tcccgccagc gcaatcggtt taaggtgttt
     2641 gtaggcttcc atcagatagt agttggcatc gccgttgtca gcgatatccg cgatattgcc
     2701 gcaagggaca atgaccgcat cgaccgtcag cgaaggtgca ccggcaaagg tagcggctat
     2761 aggcaacacc gtaccgtcat ccgcagtcac ttcacccatt cgggagtaga gcagtttggc
     2821 atgaacgcct ttggccttca tggccttgag aatggccaga aggtctgccg atctcacttc
     2881 atcattaagt aaaatcgcta ccacgcgacc tttcacatca ccgtcaggaa tggcgtacaa
     2941 acttaaggat ggatcctttt tcagaccgtt gacgtccgga ggtggggtga tattcagctg
     3001 gtcgtcagtc agttcgatac cgagattttt cgccaccgcc tgggccagag tgagatcaat
     3061 atgcgccagc tggtcaacaa cgcgctcacg aatatacgga cgaacgactt tgcttaactc
     3121 aaaactgaaa ccatcgacaa tatggcgctg ctcgaatggc gtctgactta gccagaacag
     3181 acgcggatgg gaataatatt cgccaaacga tgggctgcgc tcgcgaactt tattgccttc
     3241 cacgcgctct tggtatgatt caaaaccgcc gcgtttcggc cccggtggtg tttcgcgcgg
     3301 ccagttatcg ttgatcgagt tcggttcata attcgccggg ttagtgtcga tccccatacg
     3361 atgcatgccg tcacgctgga aattatggta agggcaggtc gggcggttaa tcggaatctc
     3421 atggaaattc ggcccaccaa gacgactgat ttgtgtatcg gtataggaga acaaacgtcc
     3481 ctgcaacagg ggatcgttgg tgaagtccag accaggaaca atatgacctg gatggaatgc
     3541 tgcttgttcg ttttcggcaa agaagttatc cggattgcga ttgagcacca ttttgccgac
     3601 acgctgaacg ggcaccagtt cttccgggat cagtttggtt ggatcgagaa gatcgaagtc
     3661 gaacttgaat tcatcttctt caggaatcaa ctggaagccc agttcgtatt ccggaaaatc
     3721 gcctgcttca atggcttccc acaactcgcg gcggtggaag tccgggtcac gtccggtgag
     3781 tttttgtgct tcatcccaaa cgagtgaggc tttacctgcc agtggtttcc agtggaaacg
     3841 tacaaacgtt gccttccctt cggcattaat caggcggaag gtgtgaatac cgaagccttc
     3901 catggtgcgg taactgcggg ggatgccgcg atccgacatc gcccacatca cgttgtgcag
     3961 agtttcaggt tgcagagaaa cataatccca gaaagtatcg tgggcacttt gcccttgtgg
     4021 aattgcccag tgcggttctg gttttaccgc atgaacaaaa tcggggaatt tatgcgcatc
     4081 ctggataaag aagattggcg tgttattgcc aacgaggtca aaaataccct cttcagtata
     4141 gaacttggtg gcaaaaccac ggatatcacg cacggtatcg gcagagccag cgccaccctg
     4201 aacggtagag aacctaacaa atactggggt gattttgttc ggatctgaga ggaaatccgc
     4261 tttggtgata tcgcttaagc ttttatatgg ctggaaataa ccgtgagctg ctgagccgcg
     4321 tgcatgaaca atacgttccg ggatgcgctc atggtcaaag tgggtgattt tctcgcgcag
     4381 aataaaatct tccagcagcg ttggaccacg gttaccggca cgcagtgagt tttgatcatc
     4441 ggcgatgcgc acgccctgat tagtggtcag cgcataattt tcactgcctt tgcgtacgtc
     4501 ttccagagaa ttaagttttt cgttacgtgt atcaggggct ttcaggctcc caggggcggt
     4561 aggttgtgca cctggcggtg ttggttcagc cgctggacga tgagatccgt cctcaggtgc
     4621 cagtgagtcc atccccggtt tcgcttcgct ggaatcgtgt agtggtgact ggtgctgatg
     4681 tgggttcttt tcgttatgtt gcgacattga actcgtctcc ttaatttatt actgaaaggg
     4741 ccgctgtttt ggtcaaaaaa ctctctaact atagaccacc agccagatcc cgcttcggag
     4801 actattctgg aaacggtgtt atgaaataca cgcaaagcgg gacgtacagg ggctaaatcg
     4861 gctaaactac agtttttcta atttttaata tgcgtttagt gaagccagta atgaagaaac
     4921 cgctccgcca gcaaaaccgc cagattatta gctatgtccc acgcacggaa cccgcgccgc
     4981 cagaacatgc gataaagatg gattcgtttc gtgatgtctg gatgctgcgt ggcaaatatg
     5041 ttgcgtttgt actgatggga gagtcatttc tgcgctcacc ggcgtttact gtgcctgaat
     5101 ctgcccaacg ttgggcaaat cagatccgcc aggaagggga agtgactgag taaggattac
     5161 atacctcccg gttttgccgg gagttacagg acctgaaatt ctgcatttcg ttgctacatt
     5221 cattcaaacc acctgtaaca aaaagctgcc tttgacgagc tattgctata aataagagat
     5281 cttttttctt taattattta tttattttaa aatggttaag ttttgctaat catgatgagt
     5341 tatttttgat agttcat
//
LOCUS       DAEBXN010000202         5350 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.203, whole genome shotgun sequence.
ACCESSION   DAEBXN010000202 DAEBXN010000000
VERSION     DAEBXN010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5350
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.203"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(75..557)
                     /locus_tag="KJL99_004136"
     CDS             complement(75..557)
                     /locus_tag="KJL99_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438650.1"
                     /translation="MIKPFLMTVAILTSCCAQAKILTNEEIVSRFISNVKTSADNDVD
                     LDTDGFRQLRISCPSPSASGAIILRKASYEFGKSIGVFDFANNASPATMTFLIPIYKD
                     DDFDSDTVGFTFAFKMPRGQFFVDVKENGNIRAGVNVNGESGVTYENCKLNMKDINDD
                     "
     gene            complement(625..2574)
                     /locus_tag="KJL99_004137"
     CDS             complement(625..2574)
                     /locus_tag="KJL99_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBD4438651.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLSLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDTTAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKEHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGYLSPDKRYTPNVSLERNQLLSLASSPATRSELKVTFDNAPPGMR
                     VIDLPKTGDPFMKITHDVGYSPFKR"
     gene            complement(2659..2994)
                     /locus_tag="KJL99_004138"
     CDS             complement(2659..2994)
                     /locus_tag="KJL99_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HBD4438652.1"
                     /translation="MAMNVTEIVLKKPVTAHNEMLHVLELREPTYDEIEALGFPFIIS
                     GEGSIKLDSQVALKYIPLLAGIPCSSAAQMAKLDIFKTSMQILRFFTQSETGSTSGND
                     STMLPGSGN"
     gene            complement(2994..3350)
                     /locus_tag="KJL99_004139"
     CDS             complement(2994..3350)
                     /locus_tag="KJL99_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HBD4438653.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(3350..4846)
                     /locus_tag="KJL99_004140"
     CDS             complement(3350..4846)
                     /locus_tag="KJL99_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HBD4438654.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHVYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVENYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            complement(4843..5004)
                     /locus_tag="KJL99_004141"
     CDS             complement(4843..5004)
                     /locus_tag="KJL99_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HBD4438655.1"
                     /translation="MFVKPLKGRSVPDPARGDLLPSEGRNVEESSYWFRRIAAGDVVR
                     VKQDKAKKS"
     gene            complement(5014..>5350)
                     /locus_tag="KJL99_004142"
     CDS             complement(5014..>5350)
                     /locus_tag="KJL99_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438656.1"
                     /translation="RDEKGQWAAYDAVHDVRRELWKALLGWMPDPQGGEIVYVGGTLL
                     DLNRYELYYQFDFTAKYEITEEDTRQAEDVNALPDLSLLSIDVDYIDPGTGPDGDIEH
                     HLEMRFPQN"
BASE COUNT         1276 a         1561 c         1150 g         1363 t
ORIGIN      
        1 aaagagaagg taaagaaggt aagtccatac tgtctccact ataaaaaaac cgccaatttc
       61 tggcggctta tatattaatc atcattaata tctttcatat tcaatttgca attttcgtag
      121 gtcactcccg actcgccatt aacattaact ccagctctga tattcccgtt ttcttttaca
      181 tctacaaaaa actggcctct tggcatcttg aacgcaaatg taaaaccaac agtatctgaa
      241 tcaaaatcat catctttata tattgggatc aaaaatgtca tcgtcgctgg tgatgcattg
      301 tttgcaaaat caaatacacc tatacttttg ccgaattcat aagaagcttt tctcaatata
      361 atcgcaccgc tggcagaagg ggatgggcac gatatcctca gttgtctgaa tccatctgta
      421 tccaagtcaa cgtcattatc tgcagatgtt ttaacgttac taataaatcg tgaaacaatc
      481 tcctcatttg ttagtatttt cgcctgagca cagcaagagg ttaatattgc aaccgtcatt
      541 aaaaatggct ttatcacaaa acaaactcca ttgttagcat gttacgcgat aattttgcct
      601 catctttcac cacaaaaaaa gaactcacct tttaaatgga gaatacccaa catcatgggt
      661 gattttcata aagggatcgc ctgttttcgg caaatcgata acgcgcattc caggcggcgc
      721 gttgtcaaac gtcaccttaa gttcgctgcg ggttgccggt gaagatgcaa ggcttaacag
      781 ctgattcctt tccagtgata cattcggggt gtagcgttta tcgggagata aatacccatc
      841 accacgtggc atctgccatc cggtcatgtc atagacaaaa tcatggaact gtttgcccca
      901 ctcgtcgaac ttatcattca gaccaaatcc actgttcagt gcatcagcca caaaattttt
      961 aacaagccag ggatgttctt tttcaaactc ctgcgccatc atgcccagtt caagcaatcc
     1021 accaatcaga ctgatctttc cgaggccctt taatttcagc cctgtttttc ctgccgccgt
     1081 tgtccattga ttctgggcga ctgttgccgc acgaatactt gccaccgcct tcacaccaat
     1141 atacagggca gaaaccgttg ctattgtttt tactgcctgt tcccatcccc ccattgctct
     1201 gacaacctga tcaacctcct gccagacttt tttcacaacc ggaccgaccg tttcccagtt
     1261 atcaattatg agatatgcac caccgacgag aagcgcaatt aatccctttg ccggcgtcat
     1321 attcatcacg ctacccatga tttttgttat gcgtgtcaat gtgccaatcg ccacgcccat
     1381 cgtcagtaat gccgcaccag ttttcgcgat ggtttttacg acctcaggat tttctctgac
     1441 aaacgtgcgc acttcttcca gaaaaggctt catctctttt atgccttcat tgagtgaagg
     1501 caggaaggtt tcccccagcg tggaagaaat cgcattgatc tggttctgca gcagcaacaa
     1561 ctgattttct gtcgtcgcgg cacgggcggc atattctttc tgcattgagc cgccatactg
     1621 ctgcgcatcc gccacacgat taaagttggt tcgcaacaaa tccagattcg tgagcagcgg
     1681 cgcaatggcc cccagggatt ccttcccgaa cagcgctttc agcacagcag cctgtttttc
     1741 tttcggcact tttgccagag aatccagtac gtgcagcatg gccccgcggg cgtctttctg
     1801 catatccgcc gccagttttt tcgggctaat gcgcaacgag cggagcactt ttttctgcga
     1861 tttggtggca gaatccctcg ctgtcagcga caacataaaa ttctttatcc ccgtcgcagc
     1921 aatttctgac tccaccccca ttccggcaat ggttgccccc attgcggcaa tctctccgga
     1981 ggccacaccc gcaacactgc ctaaagggcc aatacgggtc acaacatcag aaatcttttt
     2041 cgcacttgca ggaccggtat tgccaagata gttaatctta tccgcaagcc ctgccacctc
     2101 tccctgtgtc agtttaaagg cagtgcgcca ctgcgccatc atctgaccgg attcttctgc
     2161 cgtggtgtca aaggccacgc ccatcttcac ggcatcgtca gtaaactgca tcagctcgtc
     2221 acgggcgata ccagcctgac cacccgccgc cacaatttcg gcgatacctt ccgccgacat
     2281 gggcagttct gttgacaggt cgcgcacctg ctccgtcatt gccttaaacg cttccggcgt
     2341 atccagacca tccaccactt tccggacatc cgccattttt gattcaaggg caatggcaga
     2401 tttgaccggg agcgccagcg cccccagtac tgcggttccg gcacccgctg cgcccagtga
     2461 caggctggca aactcctttt taaaaccttt cagctggcgc tgcattccct tcagcggtgc
     2521 cgatacctta tccacggcag tgatgatcgc cttcagctga aaactgtcag ccatgcttca
     2581 tctcctcgtt aatgcggacg gcctcggctt ccagttctgt aaactgcgaa acagccaccc
     2641 gtcgcagctc cagtggattc agtttccaga accaggcaac attgtagagt cgtttccgga
     2701 ggtgcttccc gtctccgact gggtaaaaaa acgcaggatc tgcatgctgg tcttaaaaat
     2761 atccagtttt gccatctgcg ccgccgatga acacgggatc cccgccagca acgggatata
     2821 tttcagtgcc acctggctgt ccagtttaat actgccttca ccggaaataa tgaaaggaaa
     2881 acccagcgcc tcgatttcgt catacgtggg ctcgcgcaac tccagcacat gcagcatttc
     2941 gttatgcgct gtcaccggtt tttttaaaac tatttctgta acgttcattg ccattactga
     3001 taccctccct cttcgccgtg gaattcaaga tctgccgtgc cttcttcggc attatgatta
     3061 gcctctccgt gcagccatgc cgccgaaagc acatacacca tgccgtttgc cagctcagcg
     3121 gtaatcgtca tctggtctga ggtggtaatt ttgtcgaccg gaaagttttt gggcacttta
     3181 aacgtgccct taacgtaagg tgaccgccag gtctccttgt aatccacatc ccctgccatg
     3241 ccgacgatgt catcccggac attggtgttc atcggcactt caatgccacc tgtcagcgat
     3301 aactgctgac catccacttt aaaaaaacag gttccggcga tctttgccat tatgctgact
     3361 cctctgaata ctgaagacgg aactggttaa ccaccgcaaa gacacgtaac tgattaacat
     3421 aatccggcgg gaacagcgta ttcagtcggt tcgggttatc tgcatcacgc tcaactatca
     3481 gatactgttt aaacagatcg taattttcca caataccggc acgctccatc tgacgatatg
     3541 tcgccagaag ttcccctttg ataacggcag gagtgacaat cgcctgcccc ggaccaaaac
     3601 gggtaccatc atttgccagc ttgtgacgtc cgtacttgct tgtgatgacc gatttcagtt
     3661 tgcgaagaac atatgcactg gtatgcagag tttcactgtc cagatagctg ttgtctgcca
     3721 cgccatacgc attctttttg taggtggtta cggaacgctg gatctgcaac gtgccgcctt
     3781 ccacataagc cgtcgccacg ccgtgagata aaagggtctg ctgctctgtc atgatgaatc
     3841 gcttaccttt cggtgccgga agcatcccca ccagctcccc cgtctgtgtc ggacgggcag
     3901 gatcattccg gataaatacc gcttcacggg caaggcgact ggcaaccagt tcatcgacag
     3961 gcgactgggt ttctttttcg taaccggcaa gcgtgatatg ttgctgatta tgcatatctc
     4021 cggcaccaac cagctctgac agcgttccca gttttgcggt atagacatgc ccgtataact
     4081 ggcgcgcata actccagcga ccgctgctgt cattcatttc ggtcatcatc atattgatgg
     4141 aggcggcatc gttgaacggc agaccgataa agtcgaatgc ctcatcgccc atagcagcaa
     4201 cagcggcggt aagatcaggc gctccgctac cggcagcacc gacttccgtc accacccgaa
     4261 gccctgctgg caaaatctca ccaccaccag aaccataata attcagacag accggtaact
     4321 cattaccata cagcccctta tgacgggcag tcagcgtcac cacgcctgca tcagatgacg
     4381 ccgtaaacgg cagggtgata accccgttta ccgcttcctt aatcgcggtg gcaaccgcag
     4441 tggcatcatc gccattcacc acaggcacct gtacacggga gcgcccgaca tacagactca
     4501 gggtgccgct ttcctctgct tctccggcaa ccgtcaccct gaccgtcgcc gccgcccctc
     4561 tggcttccgg tactgcaata acatacagtt caccgaaagg atctgtctga cggtagactt
     4621 ccaccatacg cgccagctgg ctccccgccc cacaaatctg gcgggcataa tctgccgatg
     4681 gcatcagcac caggctgtta acctcaatgg ctgcatcgtt gctggcatgc ccgatcagta
     4741 atgcaggcgc gctggtcacc gctgtatttg ccgcagaatt gttcatctcg gcgtagaaca
     4801 acggcaccag cgtattcgac ggaacagtat taaaacttat cgtcatgatt ttttagcctt
     4861 atcctgttta acacgtacca catcacccgc cgctatacgg cggaaccagt agctgctttc
     4921 ttccacattt cgcccttcgg aaggcaaaag gtctccacgg gcagggtcag gaaccgaccg
     4981 ccctttcagg ggtttcacaa acatgaggga ctcttaattc tgagggaaac gcatttccag
     5041 atggtgctca atgtcaccat ccgggccagt accaggatcg atgtaatcca catcaatact
     5101 cagcagggat aaatccggca gggcattcac gtcctctgcc tgtcgcgtgt cttcttccgt
     5161 gatttcatac ttcgccgtaa aatcaaactg gtaatacagt tcgtaacggt tcagatccag
     5221 cagggtgcca ccgacataaa caatctcgcc cccttgcggg tccggcatcc atccaagcag
     5281 ggctttccag agttctctcc ggacatcatg aacggcgtca taggctgccc actgcccttt
     5341 ttcatcacgc
//
LOCUS       DAEBXN010000203         5340 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.204, whole genome shotgun sequence.
ACCESSION   DAEBXN010000203 DAEBXN010000000
VERSION     DAEBXN010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5340
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.204"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(35..772)
                     /gene="bamD"
                     /locus_tag="KJL99_004143"
     CDS             complement(35..772)
                     /gene="bamD"
                     /locus_tag="KJL99_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HBD4438657.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKGRLAKYEYSVAEYYTERGAWVAVVNRIEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            907..1887
                     /gene="rluD"
                     /locus_tag="KJL99_004144"
     CDS             907..1887
                     /gene="rluD"
                     /locus_tag="KJL99_004144"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HBD4438658.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1884..2615
                     /gene="pgeF"
                     /locus_tag="KJL99_004145"
     CDS             1884..2615
                     /gene="pgeF"
                     /locus_tag="KJL99_004145"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HBD4438659.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2745..5318
                     /gene="clpB"
                     /locus_tag="KJL99_004146"
     CDS             2745..5318
                     /gene="clpB"
                     /locus_tag="KJL99_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HBD4438660.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKATGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1310 a         1291 c         1458 g         1281 t
ORIGIN      
        1 ctcaagggct gccgttttgt gtttcaggtt tctgttatgt attgctgctg tttgcggcga
       61 tgattttcgc tactttttca gcttgcgcat tcatctgcat ctgacggtat gcattttcca
      121 tcagcggcag cgcatcacgc gtagcctggg tatccgggta gtcgcgcaac atgccttcta
      181 tgcggttaac gacggcaacc catgcgccac gttctgtata gtactcggcc acggagtatt
      241 catatttcgc cagacgacct ttcaggaata ccagacgttt ggtggcatcg gtagtgtact
      301 gactgttcgg atagccgcgc accagtttgg aaaagtcact aaacgcagct cgtgcatgtt
      361 gaggatcgcg atcgctacgg tcaacgccaa agaacccttg cagcgcactg tcatccagcg
      421 ccatattggt caggccacgc atgtacatga cataatcgat attcggatgg gtcgggttaa
      481 ggcgaataaa acgatcgatg gcagcctgcg ctaacggcaa atcggcgttt ttatagtagg
      541 cgtagatgag atccagctgc acctgctgcg aatacggacc aaacggatag cgattatcta
      601 acgcttccag ttgcgttatt gcctgtctcc agttaccgtc ctgcagcttt tgttgtgcag
      661 tcgcgtaaat ttcatttggc ggattatcag gtacttcttc ctttgacccc gagcaacccg
      721 ccaaaaacag gcttagtgtg gcggctgcca ccagatattt catgcgcgtc atgacgtttt
      781 gactttcctc aaaatgtaat acgggagatt ctctgttcct gcacccggtt aagaccagct
      841 acaatagcac actatattaa acggcaaagc cgtaaaaccc caacgataaa cgaagaagca
      901 gtatatatgg cacaacgagt acagctcact gcaacggtgt ccgaaaacca actcggtcaa
      961 cgcttagatc aggctttggc cgaaatgttc ccggattatt cacgttcgcg aataaaagaa
     1021 tggatcctcg accagcgcgt gctggtaaac ggcaaagttt gtgataagcc gaaagaaaaa
     1081 gtattgggtg gcgagcaggt tgccatcaac gctgagattg aagaagaagc gcgttttgaa
     1141 ccgcaggata tccctctgga tatcgtctat gaagatgaag atatcatcgt cattaataaa
     1201 ccgcgcgacc tagtggtaca tcctggcgcg ggtaacccgg atggcacggt actgaatgcg
     1261 ttgcttcatt actatccgcc cattgccgat gtaccacgtg cgggcatcgt ccatcgtctg
     1321 gataaagaca ccactggcct gatggttgtg gcaaaaaccg ttccggctca gacgcgttta
     1381 gtcgaatctt tgcaacggcg tgaaattact cgtgagtatg aagcggtggc gattggtcat
     1441 atgaccgctg gcggtacagt agatgaaccc atcagccgcc acccgaccaa acgtactcac
     1501 atggcggtgc atccgatggg caaaccggcg gtgactcact atcgcatcat ggaacacttc
     1561 cgtgtgcaca cgcgtctgcg gttgcgtctg gaaactggac gtacgcacca gatccgcgtg
     1621 catatggccc atatcactca tccgctggtg ggcgatccgg tttatggtgg ccgtccgcgt
     1681 ccgccaaaag gtgcttcgga agcatttatc tccacgctgc gtaagtttga ccgccaggcg
     1741 cttcatgcaa ccatgctgcg tctttatcac ccgatctccg gcatcgaaat ggaatggcat
     1801 gcgcctattc cacaagatat ggtggagctg attgaggtga tgcgcgccga tttcgaagag
     1861 cataaggatg aagtggactg gttatgagta agctgattgt cccgcagtgg ccgctgccaa
     1921 aaggtgttgc ggcctgtagc tccactcgta tcggcggcgt gagcttgccc ccgtatgact
     1981 cactcaacct cggtgcccat tgtggcgata acccggatca cgttgaggag aatcgcaagc
     2041 gactttttgc tgcgggcaat ttgccttcta aaccggtctg gcttgagcag gtacacggca
     2101 aagatgtgct taagcttact ggcgaacctt atgcctcaaa acgagcagat gcctcttata
     2161 gcaatacgcc tggcacggtt tgcgcagtga tgactgccga ctgcctccct gtgctgtttt
     2221 gcaatcgagc gggaacggaa gtcgccgccg ttcatgctgg ctggcgtgga ctgtgcgcag
     2281 gcgtgctgga agagacggtt tcctgttttg ctgataaacc tgaaaacatt ctcgcctggt
     2341 tagggccggc aattggtcca cgcgcgttcg aagtgggggc ggaggttcgc gaggcgttta
     2401 tggcagtaga cgctaaagca agtgcagctt tcattcagca tggtgataag tatctggcgg
     2461 atatttatca gcttgcccgg cagcgtctgg cgaacgtggg tgttgagcaa attttcggcg
     2521 gcgaccgttg tacatatacg gaaaatgaga ctttcttctc ttatcgtcgc gacaagacca
     2581 ccggtcgtat ggcaagtttc atttggctga tataacctaa agaatcaaga cgatccggta
     2641 cgcgtgattt tcttttcaca ttaatctggt caataacctt gaataattga gggatgacct
     2701 catttaatct ccagtagcaa ctttgatccg ttatgggagg agttatgcgt ctggatcgtc
     2761 ttactaataa attccagctt gctcttgccg atgcccaatc acttgcactc gggcacgaca
     2821 accaattcat cgaaccactt catttaatga gcgccctgct gaatcaggaa gggggttcgg
     2881 ttagtccttt attaacatcc gctggcataa atgctggcca gttgcgcaca gatatcaatc
     2941 aggcattaaa tcgtttaccg caggttgaag gtactggtgg tgatgtccag ccatcacagg
     3001 atctggtgcg cgttcttaat ctttgcgaca agctggcgca aaaacgtggt gataacttta
     3061 tctcgtcaga actgttcgtt ctggcggcac ttgagtctcg cggcacgctg gccgacatcc
     3121 tgaaagcaac aggggcgacc accgccaaca ttactcaagc gattgaacaa atgcgtggag
     3181 gtgaaagcgt gaacgatcaa ggtgctgaag accaacgtca ggctttgaaa aaatatacca
     3241 tcgaccttac cgaacgagcc gaacagggca aactcgatcc ggtgattggt cgtgatgaag
     3301 aaattcgccg taccattcag gtgctgcaac gtcgtactaa aaataacccg gtactgattg
     3361 gtgaacccgg cgtcggtaaa actgccatcg ttgaaggtct ggcgcagcgt attatcaacg
     3421 gcgaagtgcc ggaaggattg aaaggccgcc gcgtactggc gctggatatg ggcgcgctgg
     3481 tggctggggc gaaatatcgc ggtgagtttg aagaacgttt aaaaggcgtg cttaacgatc
     3541 ttgccaaaca ggaaggcaac gtcatcctgt ttatcgacga attacatacc atggtcggcg
     3601 cgggtaaagc cgatggcgca atggacgccg gaaacatgct gaaaccggcg ctggcgcgtg
     3661 gtgaattgca ctgcgtaggt gccacgacgc ttgacgaata tcgccagtac atcgaaaaag
     3721 atgctgcgct ggaacgtcgt ttccagaaag tgtttgttgc cgagccttct gttgaagata
     3781 ccattgcgat tctgcgtggc ctgaaagaac gttacgaatt gcaccaccat gtgcaaatta
     3841 ctgacccggc aattgttgca gcagcgacgt tgtctcatcg ctacattgct gaccgtcagc
     3901 tgccggataa agccatcgac ctgatcgatg aagcagcatc cagcattcgt atgcagattg
     3961 actcaaaacc agaagaactc gaccgactcg atcgtcgtat catccagctc aaactggaac
     4021 aacaggcgtt aatgaaagag tctgatgaag ccagtaaaaa acgcctggat atgctcaacg
     4081 aagaactgag cgacaaagaa cgtcagtact ccgagttaga agaagagtgg aaagcagaga
     4141 aggcatcgct ttctggtacg cagaccatta aagcggaact ggaacaggcg aaaatcgcta
     4201 ttgaacaggc tcgccgtgtg ggggacctgg cgcggatgtc tgaactgcaa tacggcaaaa
     4261 tcccggaact ggaaaagcaa ctggaagccg caacgcagct cgaaggcaaa actatgcgtc
     4321 tgttgcgtaa taaagtgacc gatgccgaaa ttgctgaagt gctggcgcgt tggacgggga
     4381 ttccggtttc tcgcatgatg gaaagcgagc gcgaaaaact gctgcgtatg gagcaagaac
     4441 tgcaccatcg cgtcattggt cagaacgaag cggttgatgc ggtatctaac gctattcgcc
     4501 gtagccgtgc ggggttggcg gatccaaatc gcccgattgg ttcattcctg ttcctcggcc
     4561 caacgggtgt ggggaaaact gagctttgta aggcgctggc gaactttatg tttgatagcg
     4621 acgaggcgat ggtccgtatc gatatgtccg aatttatgga gaaacactcg gtgtctcgtc
     4681 tggttggtgc gcctccggga tatgtcggtt atgaagaagg tggctacctg accgaagctg
     4741 tgcgtcgtcg tccgtattcc gtcatcctgc tggatgaagt ggaaaaagcg catccggatg
     4801 tcttcaatat tctgttgcag gtactggatg atgggcgtct gactgacggg caggggagaa
     4861 cggtcgactt ccgtaatacg gttgtcatta tgacctctaa cctcggttcc gatctgattc
     4921 aggaacgctt cggtgaactg gattatgcgc acatgaaaga gctggtgctc ggtgtggtta
     4981 gccataactt ccgtccggaa ttcattaacc gtattgatga agtggtggtc ttccatccgc
     5041 tgggtgaaca gcacattgcc tcgattgcgc agattcagtt gaaacgtctg tacaaacgtc
     5101 tggaagaacg tggttatgaa atccacattt ctgacgaggc gctgaaactg ctgagcgaga
     5161 acggttacga tccagtctat ggtgcacgtc ctttgaaacg tgctatccag cagcagatcg
     5221 aaaacccgct ggcacagcaa atactgtctg gtgaattggt tccgggtaaa gtgattcgcc
     5281 tggaagttaa tgaagaccgg attgtcgctg tccagtaatg ataaaacgag cccttcgggg
//
LOCUS       DAEBXN010000204         5239 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.205, whole genome shotgun sequence.
ACCESSION   DAEBXN010000204 DAEBXN010000000
VERSION     DAEBXN010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5239
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.205"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..141
                     /locus_tag="KJL99_004147"
     CDS             <1..141
                     /locus_tag="KJL99_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBD4438661.1"
                     /translation="RNVVERCFGRLKEYRRIATRYDKTARNYLAMVKLGCIRLFYQRL
                     RN"
     gene            400..584
                     /locus_tag="KJL99_004148"
                     /pseudo
     CDS             400..584
                     /locus_tag="KJL99_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001775038.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            727..948
                     /locus_tag="KJL99_004149"
                     /pseudo
     CDS             727..948
                     /locus_tag="KJL99_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(969..1031)
                     /locus_tag="KJL99_004150"
                     /pseudo
     CDS             complement(969..1031)
                     /locus_tag="KJL99_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            1481..2893
                     /gene="ospC1"
                     /locus_tag="KJL99_004151"
     CDS             1481..2893
                     /gene="ospC1"
                     /locus_tag="KJL99_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HBD4438662.1"
                     /translation="MNISETLNSANTQCNIDSMDNRLHTLFPKVTSVRNAAQQTMPDE
                     KNLKDSANIIKSFFRKTIAAQSYSRMFSQGSNFKSLNIAIDAPSDAKASFKAIEHLDR
                     LSKHYISEIREKLHPLSAEELNLLSLIINSDLIFRHQSNSDLSDKILNIKSFNKIQSE
                     GICTKRNTYADDIKKIANHDFVFFGVEISNHQKKHPLNTKHHTVDFGANAYIIDHDSP
                     YGYMTLTDHFDNAIPPVFYHEHQSFLDKFSEVNKEVSRYVHGSKGIIDVPIFNTKDMK
                     LGLGLYLIDFIRKSEDQSFKEFCYGKNLAPVDLDRIINFVFQPEYHIPRMVSTENFKK
                     VKIREISLEEAVTASNYEEINKQVTNKKIALQALFLSITNQKEDVALYILSNFEITRQ
                     DVISIKHELYDIEYLLSAHNSSCKVLEYFINKGLVDVNTKFKKTNSGDCMLDNAIKYE
                     NAEMIKLLLKYGATSDNKYI"
     gene            complement(2997..3146)
                     /locus_tag="KJL99_004152"
                     /pseudo
     CDS             complement(2997..3146)
                     /locus_tag="KJL99_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            3222..4919
                     /gene="ospD3"
                     /locus_tag="KJL99_004153"
     CDS             3222..4919
                     /gene="ospD3"
                     /locus_tag="KJL99_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HBD4438663.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGRLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFVENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNFLDEKHQKCYGLISDGMSIF
                     VDRHTPTSMSSIIRWPDNLLHPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELLL
                     AAKNDDGLSGLLLALQNGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLSQ
                     ALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHAD
                     AIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEAK
                     LNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHTADLTPHQASKLLAAEG
                     PNGVSGLIIAFQNRNFEAIKTYMEIIKNENITPEEIAEHLDKKNGSDFLEIMKNIKS"
     gene            5036..5155
                     /locus_tag="KJL99_004154"
                     /pseudo
     CDS             5036..5155
                     /locus_tag="KJL99_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
BASE COUNT         1767 a          981 c         1012 g         1479 t
ORIGIN      
        1 cgtaatgtcg ttgaacggtg ctttgggcgg ctgaaagaat atcgccgcat cgccacgcgt
       61 tacgacaaaa cggcgagaaa ttacctggcg atggtgaaac tgggctgcat ccgactcttt
      121 tatcaacgct tacgtaatta agggacacag cctagtctcc aggattcccg gggcggttca
      181 atcaaagcgc agaacttctg cgtcttcttc gtcaaaaaaa tcatcttcgt gaagcttcac
      241 gacatagcgg ggaagtttgc ttctttgaga ggcgggttta cgtttacggg atttagctga
      301 acgggccata taaccacctg aaagacaatg acattgcctg tttttataac ggtaattgca
      361 gaccatgaca aaccgcagcc gtcaggctgc ctactcgagt cacaaggctc tttgtgtccg
      421 atatgccggc agcgcattac ccatgaaact ggctggaacg tacaccataa ggtgaagaaa
      481 gttatggggg cggtgaagaa ttatctaacc ttgtcttgtt acatccaaat tgccatcgac
      541 agttacacag ttgtgaagcc ggctctctca aaaagggctt ataaaggctt gagcggtgta
      601 ccgggaaacc ggtatgcacc gttcttaggg gagggcagtc cagcaatgaa ctgtccttac
      661 ccgacaaaca tccaaaacga acggaacgtg ctggaatccg catataaccc gttgtaaagt
      721 gaggaaatgg acacctcact tgctcatgag aacgcccgcc tgcgggcact gttgcagacg
      781 caacaggaca ctatccacca gatggctgaa tacaaccgcc tgctctcaca gcggatggcg
      841 gcttatgctt ccgaaatcaa ccgactgaag gcgctggttg cgaaactgca gcgtatgcag
      901 ttcggtaaaa gctcagaaaa acttcgcgca aaaaccgaac ggcagattcc attttcacgg
      961 gcgatctacg ccacacaggc tctgggttgc agtgactgct ccattaaggc gattttgaac
     1021 tcataaggga agtaacaaaa aaactgcaaa agtacaataa ttatatatgg ccaaactaac
     1081 ctctattaaa aaacatcagt ttttcacact tagttgagct tcatgaaaaa atcatcgagg
     1141 atttcattgc agaatacaca ttccactaac tggaagcact ccataagagg aatcagagaa
     1201 agtatcgttt ttttatagta aaattcattg ctgttcaata aaattgatat agttattaaa
     1261 ttatgaaaat aatttcacaa aaaagattaa cttgagttct taagcatcgg attttagata
     1321 ttttgaaata tctggcatca cataacatca aaagtatata atactcttat ctaattacat
     1381 gcagtatatt gtatatatac tcaaaaattg taattcatac cctgctatat cttgtaggga
     1441 aagatattaa ttaaaactgt tttcatataa ggttcatttt atgaatatat cagaaacact
     1501 gaactcagca aatacccaat gcaatataga ttctatggat aacagattac atacattgtt
     1561 tccaaaagtg acatcagtgc gaaacgctgc acaacaaact atgccagatg aaaaaaattt
     1621 aaaagatagt gcaaatatta ttaaaagttt ctttaggaaa actatagcag cacagagtta
     1681 tagtagaatg ttctctcaag gctctaactt taaatcttta aatatagcaa ttgatgcacc
     1741 atcagacgct aaagcctcat ttaaggctat tgagcacctt gacagattat cgaagcatta
     1801 tatatctgaa ataagggaaa aacttcatcc tctttctgca gaggaactca atttgctttc
     1861 gctaattatt aattctgatt taatcttcag acatcaaagt aattctgatt tgtctgataa
     1921 aattttaaac attaagtcat tcaataaaat tcagtctgaa ggaatatgca caaaacgaaa
     1981 cacatacgct gatgatataa aaaaaatagc taatcatgac tttgtgtttt ttggcgttga
     2041 aatctctaac catcagaaaa aacaccccct gaatacaaaa catcacactg ttgattttgg
     2101 tgcaaatgcg tatatcattg atcatgactc tccatatgga tatatgacat taaccgatca
     2161 ctttgataat gctattccac ctgtttttta ccatgagcac caatcatttt tagataaatt
     2221 ttcagaggtt aataaagaag ttagtcgata cgtacatgga agtaaaggaa ttatagatgt
     2281 accaatattc aatactaaag atatgaagtt agggctcgga ttatacctga ttgactttat
     2341 tagaaaaagt gaagaccaaa gcttcaagga gttttgctat ggaaaaaatc ttgcccctgt
     2401 ggatctggat agaatcataa actttgtttt tcagccagag taccatatac ctaggatggt
     2461 aagtacagaa aacttcaaaa aagttaagat tagagaaata tccttagagg aggctgttac
     2521 agcatctaat tacgaagaaa ttaacaagca ggtcactaac aaaaaaattg ctctccaggc
     2581 tctttttctt tcgattacta atcaaaaaga ggatgtcgcc ttatatatat tatctaattt
     2641 tgagataact agacaagatg ttatttccat aaagcatgag ttgtatgata ttgagtatct
     2701 acttagcgct cataattcaa gctgtaaagt acttgagtat tttatcaata agggattggt
     2761 tgatgtaaac acaaagttca aaaaaactaa tagtggggat tgtatgttgg ataacgcaat
     2821 aaaatatgag aatgcagaaa tgataaaact attattgaaa tatggtgcaa catctgacaa
     2881 taaatatatt taatcaaaat tgaatatcgt ttagcaaaag gcagtatttg cacaaaatat
     2941 cgatatattg tttattgtca gtatggctca atgtgataat agttggaaag tttgatgggt
     3001 ttcgccccgt tgtagcggta gtcgaactgg aaggtcttca ggcactgctt acagcgatag
     3061 agcagccccc ctgaactgga atggccgttc cggatacccc cctgagtttc agagtaacgg
     3121 tgacaaacca catcaatctt tgccatcaat catccaaagg gcaaagagta caacaacact
     3181 aagtctgcgt cacaacccat caatgaaagg aatatataca tatgccatca gtaaatttaa
     3241 tcccatcaag gaaaatatgt ttgcaaaata tgataaataa agacaacgtc tctgttgaga
     3301 caatccagtc tctattgcac tcaaaacaat tgccatattt ttctgacaag aggagttttt
     3361 tattaaatct aaattgccaa gttaccgatc actctggaag acttattgtc tgtcgacatt
     3421 tagcttccta ctggatagca cagtttaaca aaagtagtgg tcacgtggat tatcatcact
     3481 ttgcttttcc ggatgaaatt aaaaattatg tttcagtgag tgaagaagaa aaggctatta
     3541 atgtgcctgc tattatttat tttgttgaaa acggttcatg gggagatatt attttttata
     3601 ttttcaatga aatgattttt cattccgaaa aaagcagagc actagaaata agtacatcaa
     3661 atcacaatat ggcattaggc ttgaagatta aagaaactaa aaatgggggg gattttgtca
     3721 ttcagcttta tgatcccaac catacagcaa ctcatttacg agcagagttt aacaaattta
     3781 acttagctaa aataaaaaaa ctgactgtag ataattttct tgatgaaaaa catcagaaat
     3841 gttatggtct tatatccgac ggtatgtcta tatttgtgga cagacatact ccaacaagca
     3901 tgtcctccat aatcagatgg cctgataatt tacttcaccc caaagttatt tatcacgcga
     3961 tgcgtatggg attgactgag ctaatccaaa aagtaacaag agtcgtacaa ctatctgacc
     4021 tttcagacaa tacgttagaa ttacttttgg cagccaaaaa tgacgatggt ttgtcaggat
     4081 tgcttttagc tttacaaaat gggcattcag atacaatctt agcatacgga gaactcttgg
     4141 aaacttctgg acttaacctt gataaaacgg tagaactact aactgcggaa ggaatgggag
     4201 gacgaatatc gggtttatcc caagcacttc aaaatgggca tgcagaaact atcaaaacat
     4261 acggaaggct tctcaagaag agagcaataa atatcgaata caataagctg aaaaatttgc
     4321 tgaccgctta ttattatgat gaagtacaca gacagatacc cggactaatg tttgctcttc
     4381 aaaatggaca tgcagatgct atacgcgcat acggtgagct cattcttagc ccccctctcc
     4441 tcaactcaga ggatattgta aatttgctgg cctcaaggag atatgacaat gttcccggac
     4501 ttctgttagc attgaataat ggacaggctg atgcaatctt agcttatggt gatatcttga
     4561 atgaggcaaa acttaacttg gataaaaaag cagagctgtt agaagcgaaa gattctaatg
     4621 gtttatctgg attgtttgta gccttgcata atggatgtgt agaaacaatt attgcttatg
     4681 ggaaaatact tcacactgca gaccttactc cacatcaggc atcaaaatta ctggcagcag
     4741 aaggcccaaa tggggtatct ggattaatta tagcttttca aaataggaat tttgaggcaa
     4801 taaaaactta tatggaaata ataaaaaatg aaaatattac acctgaagaa atagcagaac
     4861 acttggacaa aaaaaatgga agtgattttc tagaaattat gaagaatata aaaagctgaa
     4921 tattatctcg tggtgataca ctgaatttga ggtaaccggc tcatttaaac cgtctggtct
     4981 gttacctccg gttccacaaa aataatgtcc atcattttga agtggacact atcgtatgga
     5041 ataccggacc tggatcactg aagctttacg ccttcatttc gaagaacatt taccacgggt
     5101 tgtggccgga cgtcgcctgg gcgtaccaaa atcaactgtc tgtggtatgt tcgtgtgaac
     5161 cgcccccggt atcccgctca tgctggcgaa cccagacacg cagtgaaccg ccccgggttt
     5221 cctggagagt gttttatct
//
LOCUS       DAEBXN010000205         5167 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.206, whole genome shotgun sequence.
ACCESSION   DAEBXN010000205 DAEBXN010000000
VERSION     DAEBXN010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5167
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.206"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            1310..1519
                     /locus_tag="KJL99_004155"
     CDS             1310..1519
                     /locus_tag="KJL99_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438664.1"
                     /translation="MAKLGENTPHIIDATVDFMASSQAFREYLKKLPPDNTIPPALTD
                     EHVPLYIRRLEYYRLLYRPKQTESY"
     gene            complement(1585..2118)
                     /locus_tag="KJL99_004156"
     CDS             complement(1585..2118)
                     /locus_tag="KJL99_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438665.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYR
                     DISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIAT
                     IHSNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVT
                     LEDKLVEKPVTGKSRKN"
     gene            complement(2121..3047)
                     /locus_tag="KJL99_004157"
     CDS             complement(2121..3047)
                     /locus_tag="KJL99_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HBD4438666.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMP
                     EWARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHA
                     WSFAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSG
                     SLTLTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEA
                     AGRLDDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAR
                     EHGFDMRDRVSRQNRTAPERVKSPSRGFSMGF"
     gene            3224..3622
                     /locus_tag="KJL99_004158"
     CDS             3224..3622
                     /locus_tag="KJL99_004158"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438667.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLIDNEAGLTEQER
                     KLLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTK
                     TGELCYKKDRILQALKELKYDLETSPNPDA"
     gene            complement(3826..4740)
                     /locus_tag="KJL99_004159"
     CDS             complement(3826..4740)
                     /locus_tag="KJL99_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBD4438668.1"
                     /translation="MSAALQYFDENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQ
                     PHAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASV
                     KALKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLS
                     ASERRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPV
                     PLSLAECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGSKSKRSTVAT
                     SARTLKPWEALGISRAWYYQLKKRGLVE"
BASE COUNT         1436 a         1351 c         1104 g         1276 t
ORIGIN      
        1 aaaaaaaaac cgacctgggg tcggtggcaa gaatcagagt ctatgggaaa tattttagtt
       61 atcggatgct caatattcac aaacgcaaaa aatgcctcag tgatgcccta aaacaacctt
      121 tccagaagga gtccgcgctt tacgatgcac ggaagagata aaaaacccca caaaagtggg
      181 gaaccaagag aaacaacaat tacaggttgt tggttgtgtt cgaacaacga tgaagtagct
      241 acgctaaata ataattaccg tacaggccct acatattcaa cactaaattt aagatcctga
      301 cgaaggattt ataaatgcac gaaaacacat agacagacaa attcactaaa ttcatacaca
      361 aaaacaagtg tagttttcta agtaaattag attcgacaac caattagttt atccacatcg
      421 cattccactc ttgctacacc aatcacattt cttgatatgt aatagcagaa aaaataaaac
      481 cagacaccaa tagaccaaaa aacaaacatt caacaagaaa cattttgaac aatatcacaa
      541 aactgtcaca accatcatca gactattgaa aaaaactggt tgctgtttta taaaagcgac
      601 tgtaacaacc tccactctat agttccatta gtttaaagtg tcacacaaac tgagcatcca
      661 tttgttttcc ttacttttgc ccctgtattc caaggggttt ttttataact taaagattag
      721 cacaccacac aaaaacaata aaaaaaaacg acaagttata caataaaacc agtactatcc
      781 atacagctaa ttacatataa atactattaa accatctgga cattaaaaat aaactcttat
      841 aacataaaat taatgcggtt ccggtaccat atgtcaggga taatgtgcaa atggaggtat
      901 cggaactgca tttcagttca ttattaatta atgacaaaaa aaacctctgc gagcgcagag
      961 ggggtaagga atgttcccgg tcataatatg tcgatagtgt ttatatacat acagaacaag
     1021 aacaacaacc agccaactaa taaatatttt attttttaga gctgaaacct taaagctcac
     1081 ttccattcca tacattgtta taaaccttat gttgaaaact gaaagtaagt acggcagact
     1141 tattaagttg cgtgcagtct tttcacgtta tacaaacaag aacacctgcg aagttatttc
     1201 tccttacata tccccctatg ccaacggggg attttttctg gttcactaac gcataaataa
     1261 tagtgacacc ctatcattca caaaccgtga taatataagg attatcatca tggcaaaatt
     1321 aggtgaaaac accccacata ttatcgatgc tactgtagat tttatggcat caagccaggc
     1381 atttcgtgag tatctgaaaa aacttccccc tgataacaca ataccgccag ctcttactga
     1441 tgagcacgta ccgttatata tacgaaggct ggaatactat cgtctgcttt atcgaccaaa
     1501 gcagacagaa tcatattgaa aaaaacggtg aacggttcgc gaaattcttt cgcgggtcgt
     1561 gaggcgttgg acaagcgaag cgcgtcagtt tttccggctc ttccccgtga caggtttctc
     1621 caccagttta tcttccagtg tcacaacttt ctgatacacc gccgctgtgg cgttgtagtt
     1681 cctgtcgagt attgttttca ttccccatcc gagcacacag aacataacca gaaaagccac
     1741 tgacacccca cccagccatt tccagctata ccctttaagg gtgttgtatt cctgcacgat
     1801 attgctgtga attgtcgcaa tatccctgtt cagggcatcc gcgtttgcct tcatgtttgc
     1861 gactgactct tccagccgct gaaaatcctg gctgatgcgc tcacctatca gtcctgccac
     1921 gtccagcttc tgcacctcat ttctgacttc atccctgacc tgttttgcca caccgtcaga
     1981 aatatcccga tatccgcgaa gagtttccct tgccacagcc agagaatcag caacagcttt
     2041 ctgttgctcc attgcggtat gttgcagatg ctccaccgat aacaggagcg taagtaattt
     2101 ttcttctgtg ctggccatgt tcagaatccc atagaaaatc cacgtgacgg tgatttaacc
     2161 cgttcaggtg cagttcggtt ctgtctgctc actctgtctc tcatgtcaaa gccatgctcc
     2221 cgcgcaacat catgttgcag ccttctgacc agttcatgtt gcttaaccgg agtgtccagc
     2281 cattccctga tgtcatgccc gaccgttcgc gcctgttcaa gatggcggga atagtccggt
     2341 tcagaccgtt tttgcatgat gtcatgacgc tgggcatcat caagccgtcc ggcagcttca
     2401 aaacccagat gcacttcggg acgtcgttca atgccctgtt ctttcagcgt ccggcagtca
     2461 atgaagcccc tttcaaagcc atgctttctc atatgttcgt tgtgcttcac ctcccagcgt
     2521 tttcgcaatt cccgcaactg ctctttttgc tgagtgagag tcaggcttcc ggaatccttt
     2581 ttcgcacctc cccgctcggg atacctggcg ttatagcgcc ggaaatactg ttccggtgta
     2641 cgttcaatgc catcattcac acgctgactc atcatgatat gcgcatgagg ctgttcgccg
     2701 ccgtcaatgc tcgctttcgg attgtgaatg gcaaaactcc aggcgtgccg ttcgccagct
     2761 tcctgacgta caaaatcctg caccagttca aggcgctgtt ccggtgtcag ttcacggggt
     2821 aatgcaattt caagttccct ataggtcgaa ccgttcgcac gttcaaactg gtcagccgcc
     2881 tgccagaaat gggaaggatt atccctggcc cattccggca tgtttccgtg tgcggaatat
     2941 tccaaatcct gacggtcgcg gtatttctct ttgcgctcga tgtaatcggc gtggtttgca
     3001 gcctgcccct tgccaccaaa tttaacgctg aggtgatagg ttgccacggt ttcagccctc
     3061 tgcaggagcg cactgtttct cgacgaagga gagaaacata taagtgcgct tccttctaat
     3121 tttacgaatt agcacggaag ataccccctg cacccgccga ccgcaagcga tatactgcac
     3181 gaccacaaca caccgcaaaa aaacaccagg gagcatcacg aacatgcgca tcagcaacat
     3241 cgaatggctg aagaaacgga tcggattcat caggaagctc ggagaacaga ccgcacgaca
     3301 gagacagatt attgacctga tcgacaacga agccggactc acagaacagg aacgcaaact
     3361 tctccacgta ctggcaacag cggaaaaaaa cgacttacag gcacaggaga gcgaacgaaa
     3421 acaggcggtc caaaaacgaa tcgagggaaa aaaacaacga cgggaacgca atcaccggct
     3481 ctttctcgcg gctgggctac tgatcgaagc aggactggta gacacaaaaa caggcgagct
     3541 atgctataaa aaagacagga tattacaagc acttaaggaa cttaagtacg atctggagac
     3601 atccccaaac ccagacgcgt aagtgcctcc atccgtgttc cgattccctg gtgcttcctc
     3661 gctcattcgc tcgctacgct cggtcatgag actgcggcga gcactaccac cacaatcacg
     3721 cggaaagccg cgccattgca gggctgaacg gggaaaaacg gcaaaaaacc tgctgctcaa
     3781 aaagacgcca gaaaggcgct gttatctgat ataggcttat ttggtctact ctacgagacc
     3841 tcgttttttc agttggtaat accaggcgcg actgatccct aatgcctccc acggtttcag
     3901 cgtgcgtgca gatgtagcta ctgtgctgcg cttagactta gaaccgccct tgcgaccgcg
     3961 tgcagcctga atttctggcg tgtgcgtatc agccacatac tgtgcgaaag tttccggcgt
     4021 gaagttcctg tgcgtgtatt tcgcaatgct cttgccaata gcccgacatt cagccagtga
     4081 aagcggaacg ggaagcgatg cattgtacat ttcgacacgc tggatcaccg cttcaagcca
     4141 ctgtgagaac gctggccagc cctgacgaat cgccctgtaa gcccatttgc gcgtcatttc
     4201 gaacaggtgg cagtttcgcc ccatcccgta atgtttatcg atgctacgac gctctgaggc
     4261 gctcaaatcg agataatcag ccagttcatc gagggtatag gcttcctcgc gccactccat
     4321 caccagccat tccaggtgga acggattttt gcaaatcagg ccgctgtagt tcacatccgc
     4381 acaaagtttc tcacacagcg aacgttcaac cgctgcggca tatttcagcg ccttaaccga
     4441 ggcatcaggc gcggttctca cggcgatgtt gagtgcgtag agcaggtgag cgtgtccgtt
     4501 cacaggattt ttaacggtga tgttcggcgc gggagcgttc cggtcgctcc agtcaatcgc
     4561 agccccctcg cggtcaacgt caaaaaccag ccagaactgc gcatgaggct ggttctgctg
     4621 tatgtaccgc gcaagaagcg cacgcccttt gccggagatg cgaagaccaa aagcgagatc
     4681 atccgtgtga tagggacgat ggggtaaatt ttcgtcgaag tattgaagcg cggcgctcat
     4741 gtgttacgat tctctccggg ttatgcgacc tacacgccct ctgtatttca tgttgaatct
     4801 tgccggatct ggacatgatt tcaggggcgt ttgtttttca gtcgcttaca ctacacccaa
     4861 cggcaaatcc tgtcgaacct tcccgaaaac ccgatccatt taccataact cccgttgtac
     4921 gcccccctta aaacgccctc agtcgctttc tggcgcaaaa aacgcaaaaa gacaagaaaa
     4981 caccaggcca aaccagaaaa acgcgtcaga atcgttttca gggcgttgca taactatgca
     5041 cgaaagcgat gtgcgatcgg tttcgtgcgt tctgaacacc gaacggctca aaacgacaag
     5101 ttttctcccc tggtgcgtgg cgctctgacg gggtttcctg tttccaaact tcacatctcg
     5161 ccaggac
//
LOCUS       DAEBXN010000206         5164 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.207, whole genome shotgun sequence.
ACCESSION   DAEBXN010000206 DAEBXN010000000
VERSION     DAEBXN010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5164
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.207"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..220
                     /locus_tag="KJL99_004160"
     CDS             <1..220
                     /locus_tag="KJL99_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBD4438669.1"
                     /translation="LYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHW
                     GFSDQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(392..721)
                     /locus_tag="KJL99_004161"
     CDS             complement(392..721)
                     /locus_tag="KJL99_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HBD4438670.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTEINAERKASYQQRAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(726..911)
                     /locus_tag="KJL99_004162"
     CDS             complement(726..911)
                     /locus_tag="KJL99_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HBD4438671.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(908..3547)
                     /locus_tag="KJL99_004163"
     CDS             complement(908..3547)
                     /locus_tag="KJL99_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HBD4438672.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLADIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWLYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLNVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWLYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRNPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGLMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3755..4744
                     /gene="ldhA"
                     /locus_tag="KJL99_004164"
     CDS             3755..4744
                     /gene="ldhA"
                     /locus_tag="KJL99_004164"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HBD4438673.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSHPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4855..>5164
                     /gene="hslJ"
                     /locus_tag="KJL99_004165"
     CDS             4855..>5164
                     /gene="hslJ"
                     /locus_tag="KJL99_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HBD4438674.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     N"
BASE COUNT         1309 a         1322 c         1203 g         1330 t
ORIGIN      
        1 tttgtaccga atgtttgccg ataaaggttt ggtagtcgcg caatatattc gtaaccgtcg
       61 tctcgatttt tgtgcagatg cgattcgcca tgccgcagat gatgaaaaac tggcaggcat
      121 cggctttcat tggggatttt ctgaccagag tcatttttca acggtattta agcaacgctt
      181 tgggatgacg ccaggcgagt atcgacgtaa attccgctaa ctaattggcg ttgcatgtac
      241 tgcaacgcca attagttaaa acagatattt gactatgcat ttttcagggc gaaaggcaat
      301 ttgacaggag tttttgaata ttcaccttgt ttcaggaaaa tagatcaatc gctttctcct
      361 gtagcgttat cccgatattg cggttttgct attaaaactt tcgcacccat ttaccgttta
      421 tcccttgtac atattccccc ggtttggccc gagctacgag cttttgcccg gcgagtttcg
      481 cgatatcatc tacagacaca ttattttgct ttgcccgttg ttggtaactc gctttacgtt
      541 cggcattaat ttcggttact aatttctctg tttcagcatc cgttttcagc gcaaccagat
      601 aaccataaaa tgtttcacct acccgcccct gagttctggc ttcatccaga gtcaatgcca
      661 ttacattgct gcttaccagc ccaacaagaa acgcacaaag aagtaatgtt ttcttcatca
      721 tcgcctcaga aaagatcgct acgagtttca agcagctctt cgacatcttt gtctgccttg
      781 atgatgatct catgctcaat tttaacgttc atattgatag tgatcggttc cttaggtgca
      841 gcgacttcaa tgcgaggcgt acagccaacc agcataaaag atgacgtcaa cgcagccagt
      901 aaaattttca ttgtttttcc tcacactctt ttccttgcgg acagtcattt cccggcagac
      961 gtgcgttctg ctccaaccat gcctggagat tatcgccaaa gcgtaaactg cgccacagcg
     1021 taaaaatatt ctcttcatga tggtaattaa ggtttaccgt accgctttta ccatcaacgc
     1081 gactggtacc tgtaatgttg gcctgcatgg ttaataaacc gagattatct aagttaattt
     1141 ttgtcgatga ccggctaatt tccatatagc gcaaccagtt aattgctgaa cccgcagtca
     1201 tattgtcttt caccaccgca tctgcggtat ctttatccag ccgcaaggtc attagcccgc
     1261 tatttgccag ccaaccatct ttcactatcc atttttcgtt gtttagccat aacggcaatg
     1321 caccactaaa tgccccggac atggcgaatt gtttcggatt gacggcgcta accagttcgc
     1381 tggatgagag attattcagc cgcaacagcg ccgggtcatg ttgcggcata cgtaattgtt
     1441 tcatcagcac gttaccgcct aacacatcga cgctaacatc agtcaacagc aagggttctt
     1501 cttcagtcca cggataacgc ccttgcaaat cagccgtaat atttttcgct gtcaccagat
     1561 taatcacttc ggcaattcgc aacgtaacgg ggccgcgagt ccccagatgc caggctccat
     1621 cggcaaaacg gaaaggcagg acaaaatcga caccgttaac ctgattatct ggcatccagg
     1681 cactaccgcc tttcaacacg ccgtgccctc ccgcgcggaa tccttgttca ggcgcagcag
     1741 aaaatgcaac ctgagcatac agttcaccat cgcgtaagtt catcttccag tcgggtggca
     1801 ccagcggctg gaatacagtc agtgattgtt ttggccacca ggcgttaccg cgcagacgaa
     1861 taccgtccca gcgaccattt acccgaaccg ggccaatctc accagcatgt aaatcgcctt
     1921 taaagaggaa ataggtcgga ttgcgcccat cgacgctaaa ttttaaggtt gatggcggta
     1981 acacactgcc gccagtgaac agcgtttgcc cggcgtccag tgacagcgcg ccgctgaagg
     2041 agggatgctg tgcgtcacgt acccagacga cgggcttgtc gagaattaat cgcggctttt
     2101 ctaccgtcat cgtaccgtat tgtaactggt cgaaaccggt ggagagatcg gtcagcgtaa
     2161 tcgtgctgtc atgccactca ccttttcctg cgacatccca ggtggcattc atcggtgtaa
     2221 aactcccttt tccccagtag agccactgcc agcggccagc gtcagggaga aaatcattcg
     2281 ccagcccatc catatgcagc acgaaatcgc ccagttcatt ttcatgcgcc tgcaaaatgg
     2341 cctgcaaacg tccgtcaaca ccacgttggg tgacttttac acccgctaaa ggccagcgga
     2401 tttcatcgat gtccagcgaa tcgatgactc ttccctttga acgaagtaac gcgcctggct
     2461 caaaggtcag cgttgggtca gacagacttc cacttagctg cgcaggtaaa cgggcatata
     2521 aaatgaggtc cgcctgtttc gcttcaccgg tcagctgcag aggcagttga ctgttatcca
     2581 tgcttaattt tcctgggcca aaattaagca ccgcgttgcc cttacccgct tgcccctggg
     2641 tcagcacatt cagtcgtccg ctgaccagag cgttctcaag ccctgcctgc cagttgtcga
     2701 ctttgacacc cagtcggcca ctcaaaggaa aaccagcata cagccagctc cagcgaccat
     2761 cgcttacggt caattgttga cgagtaattt gccacggcaa atcgagcaac ggatcgccgt
     2821 tatcccgtgc cagcacgatc aattgcccgc tattttcctg ccagtccagc tcggcatcca
     2881 ccagtgacgg ttcctgcggc aagtttaacg tcgcagtagc atgcccactt acaggaagtc
     2941 catccggcac gagcggcata gtaaattccc ccaccagttt aaccggcggc tgattttcaa
     3001 acgcgacgac atccagttcg ctgactgtaa gttgttgccc tttcagctgg ccttgaaatt
     3061 taactttttc gccctgataa cgcagttgct ggatatcaga ggttaatgcg agagagagtt
     3121 ttccctgcca ttcctgccag ggagaaaaaa tcagtttatc gatattgatc caggtgttag
     3181 gcagcatgga ctgccactgc gcgagggttt ttggagcagc tggcgattgc tccgtctgcg
     3241 gcaatttcgc cagacaagca gaatcaagtt ctaccgtgcc gacgttcaat aaccagcggc
     3301 tgggatgtga aaggctggcg ttggtgatat gcgcaagctg acaatctccc accagataac
     3361 ggagatcggg aatgattaaa cctttacgcg taatgcgtgg gctttcatct aatgcaatac
     3421 gtgtcccgag cggtagccag atgtccgcca gcgtgggaac ccacagcccg agcgtcatca
     3481 gcagcgtcaa cggcacaaga ataatcagta ataacagcgc gagaacggct ttatatttac
     3541 ccagcatggg tagttaatat cctgatttag cgaaaaatta agcattcaat acgggtattg
     3601 tggcatgttt aaccgttcag ttgaaggttg cgcctacact aagcatagtt gttgatgaat
     3661 ttttcaatat cgccatggct ttcaattata tttgaaattt tgtaaaatat ttttagtagc
     3721 ttaaatgtga ttcaacatca ctggagaaag tcttatgaaa ctcgccgttt atagcacaaa
     3781 acagtacgac aagaagtacc tgcaacaggt gaacgagtcc tttggctttg agctggaatt
     3841 ttttgacttt ctgctgacgg aaaaaaccgc taaaactgcc aatggctgcg aagcggtatg
     3901 tattttcgta aacgatgacg gcagccaccc ggtgctggaa gagctgaaaa agcacggcgt
     3961 taaatatatc gccctgcgct gtgccggttt caataacgtc gaccttgatg cggcaaaaga
     4021 actggggctg aaagtagtcc gtgttccagc ctatgatcca gaggccgttg ctgaacacgc
     4081 catcggtatg atgatgacgc tgaaccgccg tattcaccgc gcgtatcagc gtacccgtga
     4141 cgctaacttc tctctggaag gtctgaccgg ctttactatg tatggcaaaa cggcaggcgt
     4201 tatcggtacc ggtaaaatcg gtgtggcgat gctgcgcatt ctgaaaggtt ttggtatgcg
     4261 tctgctggcg ttcgatccgt atccaagtgc agcggcgctg gaactcggtg tggagtatgt
     4321 cgatctgcca accctgttct ctgaatcaga cgttatctct ctgcactgcc cgctgacacc
     4381 ggaaaactac catctgttga acgaagccgc cttcgatcag atgaaaaatg gcgtgatgat
     4441 cgtcaatacc agtcgcggtg cattgattga ttctcaggcg gcaattgaag cgctgaaaaa
     4501 tcagaaaatt ggttcgttgg gtatggacgt ctatgagaac gaacgcgatc tgttctttga
     4561 agataaatcc aacgacgtga tccaggatga cgtattccgt cgcctgtctg cctgccacaa
     4621 cgtgctgttt accgggcacc aggcattcct gacagcagaa gctctgacca gtatttctca
     4681 gactacgctg caaaacttaa gcaatctgga aaaaggtgaa acctgcccga acgaactggt
     4741 ttaatcttgc agctcccctg caatccaggg gagctgattc agataatccc caatgacctt
     4801 tcattctcta ttcttaaaat agtcctgagt cagaaactgt aattgagaac cacaatgaag
     4861 aaagtagccg cgtttgttgc gctaagcctg ctgatggcgg gatgtgtaag taatgacaaa
     4921 attgctgtta cgccagaaca gctacagcat catcgctttg tgctggaaag cgtaaacggt
     4981 aagcccgtga ccagcgataa aaatccgcca gaaatcagct ttggtgaaaa aatgatgatt
     5041 tccggcagca tgtgtaaccg ctttagcggt gaaggcaaac tgtctaatgg tgaactgaca
     5101 gccaaagggc tggcaatgac ccgtatgatg tgcgctaacc cgcagcttaa tgaactcgat
     5161 aaca
//
LOCUS       DAEBXN010000207         5149 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.208, whole genome shotgun sequence.
ACCESSION   DAEBXN010000207 DAEBXN010000000
VERSION     DAEBXN010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5149
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.208"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            189..446
                     /locus_tag="KJL99_004166"
                     /pseudo
     CDS             189..446
                     /locus_tag="KJL99_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
     gene            complement(496..1446)
                     /gene="uspE"
                     /locus_tag="KJL99_004167"
     CDS             complement(496..1446)
                     /gene="uspE"
                     /locus_tag="KJL99_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HBD4438675.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1598..2350)
                     /gene="fnr"
                     /locus_tag="KJL99_004168"
     CDS             complement(1598..2350)
                     /gene="fnr"
                     /locus_tag="KJL99_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HBD4438676.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2545..3060)
                     /gene="ogt"
                     /locus_tag="KJL99_004169"
     CDS             complement(2545..3060)
                     /gene="ogt"
                     /locus_tag="KJL99_004169"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBD4438677.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3071..3214)
                     /locus_tag="KJL99_004170"
                     /pseudo
     CDS             complement(3071..3214)
                     /locus_tag="KJL99_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
     gene            complement(3604..4419)
                     /locus_tag="KJL99_004171"
     CDS             complement(3604..4419)
                     /locus_tag="KJL99_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBD4438678.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGTVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHNYAITRQMI"
     gene            4516..>5149
                     /locus_tag="KJL99_004172"
     CDS             4516..>5149
                     /locus_tag="KJL99_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBD4438679.1"
                     /translation="MIISKKQLIGVVAIGILLTGVVFFIWRVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLEG
                     IQATITMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     QVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLEYT"
BASE COUNT         1374 a         1175 c         1225 g         1375 t
ORIGIN      
        1 ctattttacc gcgacaattc attcagaaca tcaatagtca gggaaggaag tagcaacatt
       61 agctaactaa tcaatgcgtt gattgtaaat ccagctaaga ggtgaggttt tcagagcaga
      121 caacggtgaa acgtcatggt attgttacgt ttaggtaaca agaaatttgt ctgcacaagg
      181 attacatcat gattatggcg aaactgaagt cagcgaaagg gaagaaattt ctctttggtt
      241 tgttggcggt tttcattatt gcggcgtcgg ttgtgactcg cgcgaccatc ggcggcgtta
      301 tagaacagta caatattccg ctgtctgagt ggacgacatc aatgtatgtg atttagtcat
      361 cgatgatttt tgtttatagc ctggtcttta ctgtgttgct ggcaatcccg ttgggaattt
      421 atttccttgg cggcgaagag cagtaagtaa aaaataggcc cgataactcg ggccttgtca
      481 gttattgaag agtcgttaat cgtcttcttc gtcatccagt tcaacgggtg tctgatactg
      541 gtcaggttta atgaccagca ggtcgcagcg aagatgatca atcacctgtt ccgccgtgtt
      601 gccgaggaat gctgctgaaa taccggtgcg tccgaccgtg cccagaacca caattcccgc
      661 ctgtaaatgc tccgccaaat caggaatcac ctcttcgggc agaccttttt ctacgtgcgt
      721 catgttttca ttaatgccga atttctgccg cagggctttc attgccagca aatgttgccc
      781 acgaatggca tcgttataaa cgctcgggtc aaattccggc agttcaatcg cgatattaat
      841 tggcgttacc ggataagcgc caaccagatg aacttcggta tggttgactt gttctgccag
      901 ttcgatcgtc tctttgacca gtttttcatt gagcgcatta tgatacggct cttcactggc
      961 gagattcacc gccaccagcg ccttgcctcc ttccggccac ggctggtctt tcaccatcca
     1021 caccgggctt gggcatttgc gtaacagatg ccagtccgtt ggcgtaaaaa tcaccgcttc
     1081 cagacggtca tgttggtgcg ccatttttag caccaaatcg tgtccgccgc tgatcacttc
     1141 ctgaatgatg gcttcgaaag gacggttatg ccagaccact ttaatttcaa tgggaacgcc
     1201 agcattgaga taatattttg cctgctcgtg gatccaggct gtacgctggc tgatgacgcc
     1261 ctgacgcata gcggtacgtt cgtccgggga gagcagggtg gtcatttcgt atgagaagtc
     1321 atagatcggc aaaaaggctt taattttgcc accaatccgt tgatgtaaat aaacagctcg
     1381 ccgcaatgct ggttggtcgt cctggttagg atcgataaca acgagcatgt tctgatacat
     1441 agccatacag ggtctcctta caacaactgt caacgcagtt tgtaattaaa agattaaccc
     1501 atatctggtg aatgaaacag tgatgaacct tctgccagat caataaatca gaaaaattta
     1561 atgatatgac agaaggatag tgagttatgc ggaaaaatca ggcaacgtta cgcgtatggc
     1621 cagcaagctg ggccagcgca tcgttatttt cgatggtgat gtatttacct ttgactgcca
     1681 gcataccgct tttctggaag cgacccagca gacggctgat ggtttctacc gtcaggccca
     1741 gatagttacc aatatcgcca cgagtcatcg tcaggcggaa ttcacgaggg gagaagccgc
     1801 gttgggcaaa acgacgggac aggttgtaga tgaatgcagc cagacgttcc tcggcatttt
     1861 tcttcgacaa cagcaagatc atgtcctgat cgcctttgat ttcaccgctc atcagacgca
     1921 tcatctgctg acgcagattc ggcattttac cggacaaatc gtccagcgtt tcgaacggaa
     1981 tttcacatac catcgaggtt tccagcgcct gagcgaagct cgggtgatgg ccgctgccga
     2041 tggcgtcaaa tcccactagg tcgcctgcta aatggaaacc agtgatttgc tcgtcgcctt
     2101 gctcagtgat tgtataactt ttaatcgtac cggagcggat ggcataaagc gatttaagtt
     2161 catcaccagc cttaaacagc gtctggcctt tctgaatagg cttcttccgc tcaatgatat
     2221 tatcaagctg atcaagctca tgttcgttga gtgtgaacgg gatgcaaagc tggctgatgc
     2281 tgcaatcctg gcaatggata gcacaaccgc cagactgaat gcgccgtata attcgctttt
     2341 ccgggatcat aggtctgctc aagccgtaat tgatatttgt caattttaac atctttttag
     2401 ggagcaagta agtctaagca aaccttaaca gcagagaatt ccgatattag atgtaaatat
     2461 atgtctatct atttgaaact ccttaagttg tcaagggtaa ctttacataa aagtgtgaac
     2521 aagctggcac aaattgttta atgtttacag caaaagataa ccttcgtggc gcaataacca
     2581 ctcttttcgc tgaactccgc ctgcatatcc ggtcatggtg ccgtttcggc caataacccg
     2641 atggcaaggt acgacgatgc tgatgggatt cgatccgttt gccgcaccaa cggcacgcgc
     2701 cgcgccaggg cggcccaatt gttcagccag ttggccgtaa tgcattacct gcccgcaggg
     2761 gatagtgcgt agtgttttcc agacttcgcg ctgaaatggc gtcccccccg tggcagtggg
     2821 aagcgtatca ataatgctaa gattaccggc aaaatattca cgaagcttgt cgcttaaacc
     2881 acctgggttg gtggcagaaa tgcgctcata gccttctttg cgataatgga tgtccagcag
     2941 ctgcaccatg cgttcgctgt actcttccca ttcaaccgcc cgcaggcgaa attgctcatc
     3001 gcaaatcacc cacagtggac ccagtggcgt ggcaattttt tcttcaagta atctcagcat
     3061 ccgttctctc ttaagacaaa cgtgggtaaa taccctgacc tatcggcaga ccgacaagat
     3121 accacgccag caacatcagc agccatacca ccaaaaagat aagtggatag ggcaagacta
     3181 acgaatagta agtacccagt ttcgcgtctg gtttgtcgca gtctgatagc ataaagcgat
     3241 tttttaattt ttctgcctga agaaatgagg gcaagccatc acatggatcg agggtgggat
     3301 aattccagtc agtgaaattt taccgtaggc tatctgttat ttgattctgt ggcgtgatag
     3361 ccgccagaca ccactgaact cgtcactcat tccccaatcc cggcgagaat gtggttattt
     3421 acagagctaa aaaatgaata aaaaaataga gcctgtcgac atccgccaga ctctacagta
     3481 cacacagcag tgcatcctcg tcttaaatcc ggcctggggt cctggtgacc ggtgacagtg
     3541 ttataacggt tttacccatc cgttgccaat tttcttcagt gacagatttc acgaaaatat
     3601 aagttaaatc atttgtcttg tgattgcata attatgcatc gttaatcgac gagtaaattt
     3661 accgggcgag tagcccgttc gacgcttaaa catattgccg aacgattcag ctgaggcata
     3721 gcccagacga tgggcaattt tggtcaacga atatccatca tcaagaagcg tcactgcaga
     3781 aataacgtgc atttgctgaa cccagtggcg aaatgttaat cccgtctctt taacaaataa
     3841 gcgcatgagt gtacggctac tgacgttaat tttatccgct agattatcca gtgttggcag
     3901 atacccttgg ttgtttagca aattctcaca aagctgctgt agccgctcat catttggcca
     3961 agggagttca agtgacattt tcacccccat ttttatttcc tgaagtatca gtgtctcgag
     4021 ggctgaaaaa cgtaactgtt gtttatctgt ggtactttgc gacggtggaa gttggttaat
     4081 tgcaattagt aattccctta acaggggtga aatacccgtc atgaagcaaa ctgttcccag
     4141 cgtttcactt tccgcttctg aaaccagtac ggtttttaaa agtacgttgc ctgtcgcaac
     4201 aaacttatgc ggcatttgtg ggggcagcca aagcgcgctg ctggcatgga ctagccagac
     4261 gctttctcca gcccatacac gaacgactcc ttcagccggg taaagcaact gggcatagag
     4321 atgtttatgc cagggaactg tacctccatt cagatgttcc gaatccagtc cgataatgtg
     4381 atgccctggt gatgctttgg gttgcagtgt aattgtcatt tacagtaggt attttgtcag
     4441 aaatcatcaa ataagccatg ttaaatttta aatatgacgt atgcaagttt ccggatatat
     4501 acggtgtaaa gagtgatgat aatttctaaa aaacagctta ttggggtggt agcgatcggt
     4561 attcttttga ctggcgttgt cttttttatc tggagggtaa gcaaaggaag atttatacag
     4621 acgacggatg atgcttacat tggtggtaat atcaccacgg tggcaagtaa agtttctggt
     4681 tatatttctg ctatagaggt gcgcgataat cagagcgtaa aaaaaggcga tattatcctg
     4741 cgtctcgatg atcgggatta tcgtgcaaat gtcgccaggc ttgaggcaaa aataaaaagt
     4801 agcaaagcca atctcgaagg cattcaagcc actattacga tgcagcagtc gataattcaa
     4861 agtgccagtg aaacatggca ggcagtaaaa catgaggagc aaaagcgcct gcgagatact
     4921 gaacgttatg aaaaactcgc acaatcagcg gcaatatcgc aacaaattat tgataacgcc
     4981 aggtttgact accagcaggt cgcagcaaag gagcgcaaag ccgcgaacga tttcctggtg
     5041 gagaagcaac ggctggcagt tttatctgct caggaagaaa acgtacgtgc gagtatcgaa
     5101 gaagttcagg ccgctttgac tcaggcacta ctggatctgg aatataccc
//
LOCUS       DAEBXN010000208         5114 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.209, whole genome shotgun sequence.
ACCESSION   DAEBXN010000208 DAEBXN010000000
VERSION     DAEBXN010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5114
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.209"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(337..582)
                     /locus_tag="KJL99_004173"
     CDS             complement(337..582)
                     /locus_tag="KJL99_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438680.1"
                     /translation="MKYKEVACGKQRMHWLALQFVDENNNPVSGLNVQLEYHPRAMAA
                     ELALWARGRHTQFDPTPPPPPVGVTDSQGLVRFDDLY"
     gene            complement(1359..2201)
                     /locus_tag="KJL99_004174"
     CDS             complement(1359..2201)
                     /locus_tag="KJL99_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HBD4438681.1"
                     /translation="MSEITASRPEVVNGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEVSAMTESIRGKTALDWAMKQDFRCGCWLMEKPETAMKAITRNLDRELWRDLMQRS
                     GMLSLMDAQTRDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFKGLS
                     WDYKTNSPCKFGSKIIVNNLVRWDRWGFHLITGQQTDRLVDLERMLHLFSGKPIPDNR
                     ENITIRLDGHIQSVQGKERYEDEMFIIKYFKKGSAHITFKRLELIDRINDIIARYFPS
                     VLSA"
     gene            complement(2286..2483)
                     /locus_tag="KJL99_004175"
     CDS             complement(2286..2483)
                     /locus_tag="KJL99_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438682.1"
                     /translation="MKLLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIRTLRQLQFLQQNR"
     gene            complement(2503..2991)
                     /locus_tag="KJL99_004176"
     CDS             complement(2503..2991)
                     /locus_tag="KJL99_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438683.1"
                     /translation="MKLALTLEADSVNVQALNMGRIVVDVDGVNLSELINKVSENGYS
                     LRVVDKSDQHATSTPPPLTTLTCIRCSTAHITETDNAWLYSLSHQTNDDGETEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSRTFRRLVVTLTRRYGVSLIHLDAGAECLPGFPTF
                     DW"
     gene            complement(2988..3365)
                     /locus_tag="KJL99_004177"
     CDS             complement(2988..3365)
                     /locus_tag="KJL99_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HBD4438684.1"
                     /translation="MNTLPDTHVREASGCPSPITIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSAGAPSQLINSIDILRARRATG
                     LMTRDNYRTVNNITRGKHPEAKQ"
     gene            complement(3412..3789)
                     /gene="cbtA"
                     /locus_tag="KJL99_004178"
     CDS             complement(3412..3789)
                     /gene="cbtA"
                     /locus_tag="KJL99_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system toxin CbtA"
                     /protein_id="HBD4438685.1"
                     /translation="MSDTLPGTTPPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDVDAYHLDQAFPLLMKQLELMLTGGELNPRHQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAAPATTV"
     gene            complement(3869..4090)
                     /locus_tag="KJL99_004179"
     CDS             complement(3869..4090)
                     /locus_tag="KJL99_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HBD4438686.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWHGSTDTYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(4177..4653)
                     /locus_tag="KJL99_004180"
     CDS             complement(4177..4653)
                     /locus_tag="KJL99_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HBD4438687.1"
                     /translation="MQQLSFLPGEMTTRERSFILRALKTLDRHLHEPGVAFTSTHAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVPPSKADRLITERLVQALALVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            complement(4669..>5114)
                     /locus_tag="KJL99_004181"
     CDS             complement(4669..>5114)
                     /locus_tag="KJL99_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HBD4438688.1"
                     /translation="SACAPETDLQQLVATLVPDEQRISFWPQHFGLIPQWVTLEPRVF
                     GWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGNRAEMSPEAAG
                     IAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
BASE COUNT         1193 a         1243 c         1355 g         1323 t
ORIGIN      
        1 gctgaggagt ccccagaaaa gaagacaggc atagagtttt gtgatctact gattgcgcta
       61 acaaattcaa tgagatcacc tctatgcctg cccgttaagt atgtcaggct tttttccgta
      121 atgctctaac ttccacgcat tggtttctga catcaaataa ggctatgact ttctctccct
      181 tttaatctca ttctgatcta aaaccatatc ttaaaaaaat gcatgggtat tttaattatt
      241 ttgatgttga tctaacgcac tcaaggacca ttttattacc gattatccat tctatttaac
      301 tgttgcgata taaatgtttg ttttaggtgc tttaattcag tacaagtcat caaaccgtac
      361 cagaccctga ctgtcagtga caccgacagg tgggggcggc ggtgtggggt caaactgagt
      421 gtgtctgcct ctggcccaca gagccagctc tgccgccatt gcccgtggat gatactccag
      481 ctgcacgttg agaccggaaa ctgggttgtt gttctcatcc acgaactgca gcgccagcca
      541 gtgcatccgc tgcttaccgc aggcgacttc tttgtacttc attttgtgac tcaggcaggt
      601 gactgtcgtg ttgcctgttg cgtccatttt gcggttaaac ccttttaatt caatttgtta
      661 aattgctttc caccttttgc ggggcgcgac ttaaccaaaa tattcactgt tatcataata
      721 tgcaatgggc tggaggttgc aggtcattta gggattttct gtaagccccg ttttgcagaa
      781 cgttctatct ccgattgaga tcctgtctct cggtattacc ggtagtctgg ccataatccc
      841 ctatggatca tattgatata gtaggcataa cacccaaaag ttgactaaag ctaaatcaat
      901 cagagtgaaa aatgttcggt ttgatgttgg gggggactga ggaggggcca acgcaggtgg
      961 tatgcgtttt acttcagacc ggacaaagac cggatgtatc cgtgcagtaa tgaatccgtt
     1021 agccaggtgc tcatgtggaa atagtgtcta ttggggattt gaagtttgac agaaaaggcc
     1081 acagtgacca gaagggggct acttagaaga aatgttcagg ctgggtggaa gaggtaaagc
     1141 agtgacatca gcattcaaag tgaccggata tctaccagaa ccccagaaag caaaaaacca
     1201 gcccgtaggc tggttcttct aaatagtggt gccggactcg gaatcgaaaa tttcttttat
     1261 catattgata ttaatgcaat ttgtttggtt tgattaaaaa tgtaccctca attatacccg
     1321 cagtcccttt tgatatcgaa agggaatcac tctcgggatc aggctgagag cacagaagga
     1381 aagtacctgg ctattatatc gttaattctg tcaatcagtt ccagcctttt gaaagtgatg
     1441 tgtgcagatc ccttcttaaa gtatttaatg atgaacatct cgtcttcata gcgctcttta
     1501 ccctgaacgg actggatgtg accatccaga cgaatagtga tgttttcccg gttgtcgggg
     1561 atcggtttgc cgctgaacag atgtagcatt ctttccaggt cgacgagtcg gtcagtctgt
     1621 tgcccggtga taagatgaaa cccccaccgg tcccacctca ccaggttgtt gacgataatt
     1681 ttactgccaa atttacaggg tgagttggtt ttgtaatccc agctcagccc tttgaagacg
     1741 ttgatcactc cccgttcaaa cacttcatct ttgttctggt gcagttgttc aaatgtgctc
     1801 agaatgttcg cttcgctgat ttccggaaaa ttatcatatt ccagtgacct gtaccatgtg
     1861 tcccgggtct gcgcatccat cagactcagc atcccggaac gttgcatcag atcacgccag
     1921 agttcgcgat caagattgcg tgtgatggct ttcattgcgg tttccggttt ctccatcagc
     1981 cagcaaccgc agcgaaaatc ctgtttcatg gcccagtcca gagccgtttt acctctaata
     2041 ctttctgtca ttgctgatac ttcagccagt tgtcggataa gtgtgtcgat ttgcagcaat
     2101 gtactctttc gtacagccag aatgtggcgg atactggttg agcagataac gtccgtatgc
     2161 ccgttgacca cttccggacg agaggctgtg atttcagaca taatcgatat tcctttttgc
     2221 agataaacga aaacgccagc cgtattcagg ctggcgtcag tggaatgaag tctggtgagt
     2281 aagactcacc ggttctgttg cagaaactgc agctgacgca gggtacggat atcttccctg
     2341 gcctgttcaa tacagggcag cagtgctgcc gaatccgttt tatcctcatc gttgtcgaag
     2401 ataatccctg attcgcagtc aatattgtcc tgcagccacg caatcagaat atccagcgcc
     2461 gtttctgtgg ttaataattt catccagtgt cttttgtcct gattaccagt cgaaagtggg
     2521 gaaccccggc agacattctg cgccggcgtc cagatgaatg agactgacgc cataacgccg
     2581 ggtgagagtg acgaccagac gacggaacgt tctggacagg cccaggcgtt taagccgcag
     2641 aaccgggtac gaccatgcat cggtacgtaa cagatagccg ctgcctgtaa aatgaatcca
     2701 ttctgtttcg ccgtcgtcat tagtctggtg tgacagcgag tacagccagg cgttgtccgt
     2761 ttccgtgata tgtgcggtgc tgcaacgtat gcaggtaagg gttgttaacg gtggtggtgt
     2821 actggttgcg tgttggtcgg atttatcgac cacgcggagt gaataaccgt tttcagagac
     2881 cttgttaatc agttcactga gattaacacc atcgacgtca acgacaatgc gccccatgtt
     2941 cagtgcctgc acgttaacgc tgtcggcttc cagcgtcagg gccagtttca ttgtttcgct
     3001 tccggatgct tcccccgggt aatgttattt accgttctgt agttatcgcg ggtcatcagg
     3061 ccggttgccc ggcgtgcccg gagaatatca atgctgttga ttaactgcga cggggctcct
     3121 gcgctgaatc ccggctggtc ggtacgtacc agcgcgtatt tttcaacgag aaagttcacc
     3181 gcgtcacaca gtgaaattcc tgcctcaata tgctgctcaa tcacacgttc atcagcgaac
     3241 ggtgtgtcat tcagcgtgag gccatagtgc tggtccagca gtcgggtgag cagtgtctgc
     3301 cagatggtga tgggagacgg gcagcccgat gcctcccgta cgtgagtgtc gggtaatgta
     3361 ttcatcttaa gttctctgta acaggtggtt ataaagggga gatatccggt gttatacggt
     3421 ggttgcgggc gctgccggtg tcggataaac agccagataa acgtaaccac aggagccgag
     3481 ggtgtcggct tcgcaggtca gccctttcgc atacagtgtg acggtatgct gatggcgggg
     3541 attcagttca ccgccagtga gcatgagttc cagttgtttc atcagcagcg gaaaggcctg
     3601 gtccaggtgg tacgcatcta cgtcgctgaa cctgcctctg ataccggcgc ggtctgcaag
     3661 gtaatgcagg cggttaccct cctgtaccag tcgggccccg aaacagggcg tcacggtgca
     3721 gggcagcccc caccaggggc ggtcgtgatt gtcgtcggga ggcgttgtcc cggggagtgt
     3781 gtctgacacg ataaagtcct cacagaaaat cggttaagaa tggtccggta gtggcgataa
     3841 ttctgctcat cagaattccc ttccagactc agttcagggt gacgctcatc agccggacat
     3901 acggaccaaa actgtcctta cggcgttctg caaacacagc cagtacaccg ggaatatcct
     3961 gcacttcacg gccggtatat gtatccgtgc taccgtgcca gcggtattta ccggtacaga
     4021 acggaaaaag acgggatgca ggatgctgac ggtgaatacg catggcttca ccacgggtga
     4081 tgattttcat gagaatagtc ctcctgaatg tgaaagtaag aaacagctga gaaagagaaa
     4141 ctgaagtgat atgaaggtga ttgtgacggt gacgggttaa agcagaccgt gttccgcaaa
     4201 ggagaaaacc tggctgccac cgactatcag atggtccggt acccggatat caaccagggc
     4261 cagtgcctgt accagacgct cagtgataag gcggtctgcc ttgctggggg ggacttcacc
     4321 ggacgggtga ttgtgcgcca gcaccacggc agcggcattg tggtacaggg cgcgtttaat
     4381 cacttcccgg ggatggactt ccgtacggtt gatggtgccg gtgaagaggg tttcaccggc
     4441 aatcagctga ttctggttgt tcagatacag cacccggaac tcttcacgct ccagtcccgc
     4501 catgttcaga atcagccatt cccgtgccgc atgcgtggag gtgaaggcca cgccgggttc
     4561 atgaagatgg cggtccaggg ttttcagggc ccgcagaatg aagctgcgct ctctggttgt
     4621 catttctccg ggcagaaagg aaagctgctg cattgtccct gccaccattc agtcgatgat
     4681 gcgcataatg gcgctgcatt ccggatgctg cagggcgtaa tcccgcagcc ggtaataatg
     4741 gaccgtcatg gcataacact ccgtacgaca ggcatgatga ctgtacgtca tcagacaggc
     4801 agcaatgccg gcagcttccg gactcatttc agcgcggtta ccgttcatgg cattgaacag
     4861 tacccatgtt tcgtcatcat cgtcatccgg ttcgggtgcc ataaatgccc cgccgttgtt
     4921 cagggtgtac agattccaga taccaccgca gtagtcttcg cacagacggt ccatccagcc
     4981 gaagacacgg ggctccaggg tcacccactg tggaatgagg ccaaagtgct gcggccagaa
     5041 gctgatgcgc tgttcatcag ggacgagggt ggcaaccagt tgttgcaggt cagtctccgg
     5101 tgcacaggcc gaag
//
LOCUS       DAEBXN010000209         5024 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.210, whole genome shotgun sequence.
ACCESSION   DAEBXN010000209 DAEBXN010000000
VERSION     DAEBXN010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5024
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.210"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            48..122
                     /gene="tnaC"
                     /locus_tag="KJL99_004182"
     CDS             48..122
                     /gene="tnaC"
                     /locus_tag="KJL99_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HBD4438689.1"
                     /translation="MNILHVCVTSKWFNIDNKIVDHRP"
     gene            343..1758
                     /gene="tnaA"
                     /locus_tag="KJL99_004183"
     CDS             343..1758
                     /gene="tnaA"
                     /locus_tag="KJL99_004183"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HBD4438690.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1849..3096
                     /gene="tnaB"
                     /locus_tag="KJL99_004184"
     CDS             1849..3096
                     /gene="tnaB"
                     /locus_tag="KJL99_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HBD4438691.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNIDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3228..4403
                     /gene="mdtL"
                     /locus_tag="KJL99_004185"
     CDS             3228..4403
                     /gene="mdtL"
                     /locus_tag="KJL99_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HBD4438692.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTFSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4378..>5024
                     /gene="yidZ"
                     /locus_tag="KJL99_004186"
     CDS             4378..>5024
                     /gene="yidZ"
                     /locus_tag="KJL99_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HBD4438693.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTQLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDT"
BASE COUNT         1144 a         1208 c         1256 g         1416 t
ORIGIN      
        1 tctgagtgta ataatgtagc ggcgtgtctt tcgaggataa gtgcattatg aatatcttac
       61 atgtatgtgt gacctcaaaa tggttcaata tcgacaataa gattgtcgat caccgccctt
      121 aatttaccct tctgtagcca tcaccagagc caaaccgatt agattcaatg tgatcgatta
      181 gtttgctata tcttaatttt gccttttgca aaggccatct ctcgtttatt tacttatttt
      241 agtaaatgat ggtgcttgta tatatacatg gtgaattaat ggatattgca gatgtaatat
      301 tcacagggat cactgtaatt aaaataaatg aaggattatg taatggaaaa ctttaaacat
      361 ctccctgaac cgttccgcat tcgtgttatt gagccagtaa aacgtactac tcgcgcttat
      421 cgtgaagaag caattattaa atccggtatg aacccgttcc tgctggatag cgaagatgtg
      481 tttatcgatt tactgaccga cagcggcacc ggggcggtaa cccaaagtat gcaggcagcg
      541 atgatgcgcg gcgacgaagc ctacagcggc agccgcagct actatgcgtt agccgagtca
      601 gtgaaaaata tctttggtta tcaatatact attccgactc accagggccg tggcgcagag
      661 caaatctata ttccggtact gattaaaaaa cgcgagcagg aaaaaggcct ggatcgcagc
      721 aaaatggtgg cattctctaa ctatttcttt gataccacgc agggccatag ccagattaac
      781 ggctgtaccg tgcgtaacgt ctatatcaaa gaagccttcg atacgggcgt gcgttacgac
      841 tttaaaggca actttgacct cgaaggatta gaacgcggta ttgaagaagt tggcccgaat
      901 aacgtgccgt atatcgttgc aaccatcacc agtaactctg caggtggtca gccggtttca
      961 ctggcaaact taaaagcgat gtacagcatc gcgaagaaat acgatattcc ggtggtaatg
     1021 gactccgcac gctttgctga aaacgcctat ttcatcaagc agcgtgaagc agaatacaaa
     1081 gactggacca tcgagcagat cacccgcgaa acctacaaat atgccgatat gctggcgatg
     1141 tccgccaaga aagatgcgat ggtgccgatg ggcggcttgc tgtgcatgaa agacgacagc
     1201 ttctttgatg tgtacaccga gtgcagaacc ctttgcgtgg tgcaggaagg cttcccgaca
     1261 tatggcggct tggaaggcgg cgcgatggag cgtctggcgg taggtctgta tgacggcatg
     1321 aatctcgact ggctggctta tcgtatcgcg caggtacagt atctggtcga tggtctggaa
     1381 gagattggcg ttgtctgcca gcaggcgggc ggtcacgcgg cattcgttga tgccggtaaa
     1441 ctgctgccgc atatcccggc agaccagttc ccggcacagg cgctggcgtg cgagctgtat
     1501 aaagtcgccg gtatccgtgc ggtagaaatt ggctctttcc tgttaggccg cgatccgaaa
     1561 accggtaaac aactgccatg cccggctgaa ctgctgcgtt taaccattcc gcgcgcaaca
     1621 tatactcaaa cacatatgga cttcattatt gaagccttta aacatgtaaa agagaacgcg
     1681 gcgaatatta aaggattaac ctttacctac gaaccaaaag tattgcgtca cttcaccgca
     1741 aaactgaaag aagtttaatt aatactacag agtggctata aggatgttag ccactctctt
     1801 accctacatc ctcaataaca aaaatagcct tcctctaaag gtggcatcat gactgatcaa
     1861 gctgaaaaaa agcactctgc attttggggt gttatggtta tagcaggtac agtaattggt
     1921 ggaggtatgt ttgctttacc tgttgatctt gccggtgcct ggtttttctg gggtgccttt
     1981 atccttatca ttgcctggtt ttcaatgctt cattccgggt tattgttatt agaagcaaat
     2041 ttaaattatc ctgtcggctc cagttttaac accatcacca aagatttaat aggtaacacc
     2101 tggaacatta tcagcggtat taccgttgcc ttcgttctct atatcctcac ttatgcctat
     2161 atctctgcta atggtgcgat cattagtgaa acgatatcaa tgaatttggg ctatcacgct
     2221 aatccacgta ttgtcgggat ctgcacagcc attttcgttg ccagcgtatt gtggataagt
     2281 tcgttagccg ccagtcgcat tacctcattg ttcctcgggc tgaagattat ctcctttgtg
     2341 atcgtgtttg gttctttctt cttccaggtc gattactcca tcctgcgcga tgccaccagc
     2401 accactgcgg gaacgtctta cttcccgtat atctttatgg ctttgccggt gtgtctggcg
     2461 tcatttggtt tccacggcaa tattcccagc ctgattattt gctatggcaa acgcaaagat
     2521 aagttaatca aaagcgtggt atttggttcg ctgctggcgc tggtgattta tctcttctgg
     2581 ctctattgca cgatggggaa tattccgcgc gaaagcttta aggcgataat ctcctcaggc
     2641 ggcaacattg attcgctggt gaaatcgttc ctcggcacca aacagcacgg cattatcgag
     2701 ttttgcctgc tggtgttctc taacttagct gttgccagtt cgttctttgg tgtcacgctg
     2761 gggttgttcg attatctggc ggacctgttt aagattgata actcccacgg cgggcgtttc
     2821 aaaaccgtgc tgttaacctt cctgccacct gcgttgttgt atctgatctt cccgaacggc
     2881 tttatttacg ggatcggcgg tgccgggctg tgcgccacca tctgggcggt cattattccc
     2941 gcagtgcttg caatcaaagc tcgcaagaag tttcccaatc agatgttcac ggtctggggc
     3001 ggcaatctta ttccggcgat tgtcattctc tttggtataa ccgtgatttt gtgctggttc
     3061 ggcaacgtct ttaacgtgtt acctaaattt ggctaaatcc ttcaagaagc cagccattcg
     3121 ctggcttctt gcctctcagg aaatcactta tgtccaaatg gcaactcgcc ttatcctcct
     3181 tcaccacgta tgctttgcgt caccttacta tcaggacgct ttagcccatg tcccgctttt
     3241 tgatttgtag ttttgccctg gttttacttt atcccgccgg gattgatatg tacctcgttg
     3301 gtttaccgcg catcgccgcc gatctcaatg ccagcgaagc gcagttgcat attgcgttct
     3361 ccgtatatct ggcggggatg gcagctgcga tgctatttgc cggtaaagtg gccgatcgtt
     3421 cagggagaaa gccggtcgcc atacccggcg cggcgctatt tattattgcc tcggtgttct
     3481 gttcactggc tgaaaccagc acgttatttc ttgcaggccg atttctacag gggttgggcg
     3541 caggctgttg ttacgtggtg gcgttcgcca ttttgcgcga cacgctggat gatcgacgtc
     3601 gggctaaagt gctgtcatta ctcaacggta ttacctgcat cattccggtg ttagcgccag
     3661 tgctcggaca tctgattatg cttaaattcc cgtggcagag tctgttctgg gcgatggcaa
     3721 tgatgggcat cgcggtactg atgttgtctt tgtttatttt aaaagaaacg cgcccagcgt
     3781 cccccgctgc ttcggacaaa ccacgagaaa atagcgagtc gctgcttaat cgttttttcc
     3841 tcagccgtgt tgttatcacc accctcagcg tttcggtgat cctcactttc gtcaatacat
     3901 cgccggtatt gctgatggaa atcatggggt ttgagcgcgg agaatacgcc accattatgg
     3961 cgctgaccgc tggcgtcagc atgacctttt cattctcgac gccatttgcg ctgggaattt
     4021 ttaagccacg tacgttgatg atcacctcgc aggtgttatt ccttgcggcg gggatcaccc
     4081 ttgccgtttc accttcccat gcggtttctc tgtttggtat cacgctgatt tgcgccggtt
     4141 tctcggtagg ttttggcgta gcgatgagtc aggcgttagg accattttca ttacgcgcgg
     4201 gcgtagccag ctcgacctta ggtattgcgc aggtttgcgg ttcgtcactg tggatttggc
     4261 tggccgcggt ggttggtatc ggcgcatgga atatgctgat cgggattctg attgcctgta
     4321 gcatagtgag cctgttgctg attatgttcg tcgcgcctgg acgccccgtt gccgctcatg
     4381 aagaaatcca tcaccacgct tgatctcaat ctgctgctct gtctgcaact gctgatgcag
     4441 gagcgcagcg tgaccaaagc ggcgaagcgg atgaacgtga ccccttcagc ggtgagtaag
     4501 tcgctggcaa agttaagagc gtggtttgac gacccgctct ttgtgaactc accgctgggg
     4561 ctgtcgccca cacagctaat ggtcagcatg gagcaaaatc tggcggaatg gatgcaaatg
     4621 agcaatctgc tgctggataa accgcaccac cagacaccgc gcggcctgaa gtttgagttg
     4681 gcggcggaat cgccgctgat gatgatcatg cttaatgcgc tgtcgaaacg gatctaccaa
     4741 cgttacccgc aggcgaccat caaactacgt aactgggatt acgattcctt agatgccatt
     4801 actcgtggtg aagtggatat tggtttttcc ggtcgcgaaa gccatcctcg ctcgcgggag
     4861 ctgttaagct cgctaccgtt agccattgat tatgaagtgc tgtttagtga tgtaccctgc
     4921 gtctggttac gcaaagatca tccggcactg catgaaacgt ggaatctgga caccttttta
     4981 cgttatccgc atatcagcat ttgctgggaa cagagcgaca cctg
//
LOCUS       DAEBXN010000210         4974 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.211, whole genome shotgun sequence.
ACCESSION   DAEBXN010000210 DAEBXN010000000
VERSION     DAEBXN010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4974
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.211"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(186..770)
                     /gene="kptA"
                     /locus_tag="KJL99_004187"
     CDS             complement(186..770)
                     /gene="kptA"
                     /locus_tag="KJL99_004187"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HBD4438694.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADINKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGDRHYVHLSADEATARKVGARHGSPVVLTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEWSFMPTGVQKQ"
     gene            1020..1703
                     /locus_tag="KJL99_004188"
     CDS             1020..1703
                     /locus_tag="KJL99_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HBD4438695.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFPGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGVGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            1700..2161
                     /locus_tag="KJL99_004189"
     CDS             1700..2161
                     /locus_tag="KJL99_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HBD4438696.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATADALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            2174..3346
                     /gene="iadA"
                     /locus_tag="KJL99_004190"
     CDS             2174..3346
                     /gene="iadA"
                     /locus_tag="KJL99_004190"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HBD4438697.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALKPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNMSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            3411..4322
                     /gene="hypT"
                     /locus_tag="KJL99_004191"
                     /gene_synonym="qseD"
     CDS             3411..4322
                     /gene="hypT"
                     /locus_tag="KJL99_004191"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HBD4438698.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMVRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="KJL99_004192"
     CDS             complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="KJL99_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HBD4438699.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(4704..4787)
                     /gene="idlP"
                     /locus_tag="KJL99_004193"
     CDS             complement(4704..4787)
                     /gene="idlP"
                     /locus_tag="KJL99_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HBD4438700.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
BASE COUNT         1176 a         1305 c         1240 g         1253 t
ORIGIN      
        1 gtatgaccgg aagcgacgct gattccagtc cccaactgac cgcctgccgt aaatcacttt
       61 aatttcagca gttgttatta aaggataccc ttttacgagg gtatcccttc agactgataa
      121 caaacgctac attgccggat gtgctgcgct taccaggcct atataacccc tgcaatatat
      181 tgaatttatt gtttttgtac cccagtcggc ataaacgacc actcaagaaa ctcaacagca
      241 accgttgatg tcagccagac cccgttttcc gcttgccaga aagggatgcc tcgtttcgcc
      301 atttcctgtg ctttgacggt taagacaacc ggcgatccgt gtcttgctcc cactttgcgt
      361 gccgttgctt catcagcaga gagatgcacg taatgacgat cccctgcaat cagcccctgt
      421 ttttttatct cgtcaaggaa acggcttgcc gtaccgtggt agagaaactg tggcggtgtt
      481 ttttcggcaa aggaaatagc gacctgcgaa gtcgaatgcc cctgaaccgc gcggatgcac
      541 ctaccatcac tggaataact aaaacgcttt ttatcgctgg ttgcgaccac ggtatccagc
      601 aatgcacggg ttaatcgctt gcccgctttt tgggcacaaa gtatgagctt attgatatcc
      661 gcccatcctt cacggtccag tacgatacca atcgcctccg gtttgtgtcg gaggacaaaa
      721 cttaaaaact tgctcgtatc cgccagctct ttttcgttgt actttgccat cgcgttttcg
      781 tccctggatt ttctgttttt atttactaat gtcattgtca ctgggtttat gaattttctg
      841 caactcaatc ctgcgcagaa ccttttccac acagcgtaat ttttccccta tgcaaaaaac
      901 gcatagccag aaacatactt gcattttccc catacccaca tggctgataa ggtttttcag
      961 ttccaggaat tttcctggct caagcaacac acaaacacaa aaaaacaatg gggattgtta
     1021 tgacacaaca aggggatgct gtagccgggg agctggcgac agaaaaagtg ggcattaaag
     1081 ggtatctggc tttttttctc accataatct tttttcccgg tgttttttcc ggaacagaca
     1141 gctggtggcg cgtttttgat ttttccgttc tgaacggatc gtttgggcaa ctgcctggtg
     1201 cgaatggagc caccacctca tttcgcgggg tgggtggcgc gggggctaaa gatggctttc
     1261 tctttgcgct ggagctggca ccgtcagtca ttctctctct ggggattatt tcaattaccg
     1321 atggtcttgg cggattacgc gccgcgcagc agttaatgac acctgtgcta aaaccgttgt
     1381 tgggcattcc agggatctgc tcgctggcac tgatcgctaa cctgcaaaac acggatgccg
     1441 ccgccggtat gaccaaagag ctggcgcagg aaggtgaaat taccgaacgc gataaagtga
     1501 ttttcgccgc atatcaaacc agcggtagcg ccatcatcac caactacttc tcttccggcg
     1561 tcgcggtgtt tgcctttctg ggcacgtccg taatcgtacc tttagccgtc atcctggtgt
     1621 ttaaatttgt cggagccaac attttgcgcg tctggcttaa cttcgaagaa cgtcgcaacc
     1681 caacgcaagg agcgcaagca tgacaactca ggtccgcaaa aacgtcatgg atatgtttat
     1741 cgatggcgcg cgccgtggct ttactatcgc cacgaccaac ctgctgccaa acgtggtgat
     1801 ggcgtttgtc atcattcagg cgctgaaaat taccggcctg ctcgactggg tggggcatat
     1861 ctgtgaaccc gtaatggcgc tgtggggatt acccggtgaa gcggcaaccg tccttttagc
     1921 cgcattaatg agtatgggcg gcgcagtcgg tgttgccgcc agtctggcaa ccgctgacgc
     1981 attaaccggc cacgatgtca ccgttttact gcccgcaatg tatttaatgg gcaacccggt
     2041 gcaaaatgtc ggtcgctgcc tggggacagc cgaagtgaat gccaaatatt acccgcatat
     2101 cattacggtc tgcgtgatta acgcattgct gtcgatctgg gttatgcagc ttattgttta
     2161 acaaggagtt accatgattg attataccgc agccggtttt accctgctgc agggagcgca
     2221 tttgtatgcg ccggaagatc ggggaatttg cgatgtcctc gtcgctaacg gcaaaattat
     2281 cgccgttgcc agcaatatcc cttctgacat tgtaccgaac tgcacggttg tcgatctcag
     2341 cgggcagatc ctctgcccag gttttattga tcaacacgtc catttgattg gcggtggcgg
     2401 cgaagcaggt cccacgacgc gcacgccgga agtggcgcta agtcgcctga cggaagcggg
     2461 cgtcacgtca gtggttggtc tgctgggcac cgactctatc tctcgccacc cggaatccct
     2521 gctcgccaag acccgtgcgc tcaatgaaga aggcatcagc gcctggatgc tgaccggcgc
     2581 ttatcatgtc ccttcccgca ctattacggg ttccgtggaa aaagacgtgg cgattatcga
     2641 tcgtgtgatt ggcgtgaaat gcgccatctc tgatcaccgt tctgccgcac cggacgttta
     2701 tcacctggct aatatggcgg cagaatcccg cgttggcggt ttgctcggcg gtaaacctgg
     2761 cgtcaccgtg ttccacatgg gcgacagtaa aaaggcgtta aagcctattt atgacctgct
     2821 ggaaaactgc gatgtgccga tcagcaagct gctgccgacc cacgttaacc gcaacgtacc
     2881 gttgtttgag caggcgctgg agttcgcgcg caaaggcggc accatcgata tcaccagcag
     2941 cattgacgaa ccggtcgccc ctgccgaagg tattgcccgc gccgttcagg cgggtattcc
     3001 gctggcacgc gtcaccctca gctccgacgg caacggtagc cagccgttct tcgatgacga
     3061 agggaattta acccatatcg gtgttgcagg ttttgaaacg ttgctggaaa ccgtgcaggt
     3121 gctggtcaaa gactatgatt tcagtatcag cgatgccctg cgcccgctca ccagtagcgt
     3181 agccggtttc cttaacatga gcgggaaagg cgaaattctg ccaggcaatg acgcagactt
     3241 actggtcatg acgccagaat tgcgcattga gcaggtatac gctcgcggca aactgatggt
     3301 caaagacggc aaagcctgcg tgaaaggaac gtttgaaacg gcttaaacgg ttgtcagccg
     3361 ccctaatgga cgtagaatgc ccccaagggc ggctgacaga gtaaaacgta atggatgact
     3421 gtggtgcggt tttgcataat attgaaacca aatggcttta tgattttctg actctggaaa
     3481 aatgccgcaa tttttcccag gcggcagtca gtcgcaacgt ctcgcaaccg gcattcagcc
     3541 gccgcatccg tgcactggaa caggcgattg gcgttgaatt gtttaaccgc caggtgacgc
     3601 cactgcaact ctcagaacaa gggaaaatct tccattcgca gatccgccat ctgttgcaac
     3661 agttagaaag caaccttgca gagctgcgtg gcggcagcga ttacgcgcaa cgtaaaatca
     3721 agatagccgc tgcacactct ctttccctcg gtctgttacc gtccattatc agccagatgc
     3781 cgccgctctt tacctgggca attgaagcca ttgatgtcga tgaagcggtc gataaactgc
     3841 gtgaagggca aagtgactgt attttttcct ttcacgacga agatttgctg gaagcgccgt
     3901 ttgaccacat tcgcttattt gaatctcaat tgttccctgt ctgcgccagt gacgaacacg
     3961 gagaagcact ttttaacctc gcgcagccac actttccgtt actgaattac agccgcaact
     4021 cctacatggt gcgattgatt aatcgcaccc tgacgcgcca cagtgagtta agtttcagca
     4081 ccttttttgt ctcttcgatg agcgagcttt taaagcaggt tgccctcgac ggctgtggga
     4141 ttgcctggct gccggagtat gccatccaac aagaaattcg cagcgggcag ctcgttgtgc
     4201 tgaatcggga cgaacaggtg atcccgattc aggcctacgc ataccggatg aatacccgca
     4261 tgaatccagt tgccgaacgc ttctggcgtg aactgcgcga gctggagatt gtgcttagct
     4321 gacattctca agcgtcgcac tgcgccaacc gccattatcc cagaccagat caaacgttag
     4381 cggttcatcg tcgtggtaca gcattatctc aaggctgatc gccagagtgt ggcagtacgc
     4441 ttctttcaaa ttaacctgca acgccgcaat ccgtggctca tgaaataaca gtgcagaacg
     4501 cagctgacgg caataccagc tctggagatg tttgtcgctc gggttatgca cttgccactc
     4561 gggcatgcct gaaacaagta atgacggtaa tgccgaatga ggagaattaa aaagagtgag
     4621 gagatcgctg cataccgatt tacgcagcaa tgacgtttta ttgcctgaaa tttcaggtaa
     4681 aacagcctga agtgattggc gcatcatttt gcgcactcct gacgtttagc aacatatgcg
     4741 gcttgccctg aacaccgggc accactgtat tgatgctcat tttccatagc cattcccttt
     4801 tgttcattac atccatgtca gtctgagaca ggcctgaatc ctgtcagcaa agtataaaac
     4861 cttacttgcc tatgaatatc tactgacttt tcaggaaaaa agtcatgatt tgataaaaat
     4921 catcaaactc aaaaaaaaaa cgctagtata ttctgaatat tccgaatagt tacg
//
LOCUS       DAEBXN010000211         4966 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.212, whole genome shotgun sequence.
ACCESSION   DAEBXN010000211 DAEBXN010000000
VERSION     DAEBXN010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4966
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.212"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            238..1182
                     /gene="leuO"
                     /locus_tag="KJL99_004194"
     CDS             238..1182
                     /gene="leuO"
                     /locus_tag="KJL99_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HBD4438701.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            complement(1337..1381)
                     /locus_tag="KJL99_004195"
     CDS             complement(1337..1381)
                     /locus_tag="KJL99_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438702.1"
                     /translation="MKRNDKILHYEGLN"
     gene            1500..3224
                     /gene="ilvI"
                     /locus_tag="KJL99_004196"
     CDS             1500..3224
                     /gene="ilvI"
                     /locus_tag="KJL99_004196"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HBD4438703.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVCPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGMGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRALQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3227..3718
                     /gene="ilvN"
                     /locus_tag="KJL99_004197"
     CDS             3227..3718
                     /gene="ilvN"
                     /locus_tag="KJL99_004197"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HBD4438704.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVYVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            3730..3816
                     /locus_tag="KJL99_004198"
     CDS             3730..3816
                     /locus_tag="KJL99_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HBD4438705.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            3898..4902
                     /gene="cra"
                     /locus_tag="KJL99_004199"
     CDS             3898..4902
                     /gene="cra"
                     /locus_tag="KJL99_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HBD4438706.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
BASE COUNT         1206 a         1133 c         1308 g         1319 t
ORIGIN      
        1 cggtagttat gattagattg ttttcgcaac aaaacattat ggattattat gctgtggtaa
       61 atgactcatt ccacggcaat ggattctgtt tttatcagaa cccgtatctt tatgttttcc
      121 gaattttact cattttgctt tttcttattt tatatgcatg ataaatcata ttcttcagga
      181 ttatttctct gcattccaat aagggaaagg gagttaagtg tgacagtgga gttaagtatg
      241 ccagaggtac aaacagatca ttcagagacg gcggagttaa gcaagccaca gctacgcatg
      301 gtcgatctca acttattaac cgttttcgat gccgtgatgc aggagcaaaa cattacccgt
      361 gccgctcatg ttctgggtat gtcgcaacct gcggtcagta acgctgttgc acgcctgaag
      421 gtgatgttta atgacgagct ttttgttcgt tatggccgtg gtattcaacc gactgctcgc
      481 gcatttcaac tttttggttc agttcgccag gcattgcaac tagtacaaaa tgaattgcct
      541 ggttcaggtt ttgaacccgc gagcagtgaa cgtgtatttc atctttgtgt ttgcagcccg
      601 ttagacagca ttctgacctc gcagatttat aatcacattg agcagattgc gccaaatata
      661 catgttatgt tcaagtcttc attaaatcag aacactgaac atcagctgcg ttatcaggaa
      721 acggagtttg tgattagtta tgaagacttc catcgtcctg aatttaccag cgtgccatta
      781 tttaaagatg aaatggtgct ggtagccagc aaaaatcatc caacaattaa aggcccgtta
      841 ctgaaacatg atgtttataa cgaacaacat gcggcggttt cgctcgatcg tttcgcgtca
      901 tttagtcaac cttggtatga cacggtagat aagcaagcca gtatcgcgta tcagggcatg
      961 gcaatgatga gcgtacttag cgtggtgtcg caaacgcatt tggtcgctat tgcgccgcgt
     1021 tggctggctg aagagttcgc tgaatcctta gaattacagg tattaccgct gccgttaaaa
     1081 caaaacagca gaacctgtta tctctcttgg catgaagctg ccgggcgcga taaaggccat
     1141 cagtggatgg aagaacaatt agtctcaatt tgcaaacgct aactgaatgc agaataggcc
     1201 agacatgaat gtctggttta ttctgcattt tttattgaat gtagaatttt attctgaatg
     1261 tgtggactct ctattttagg attaattaaa aaaatagaga aattgctgta agttgtggga
     1321 ttcagccgat ttattatcaa tttaatcctt cgtaatggag gattttatcg tttcttttca
     1381 cctttcctcc tgtttattct tattaccctg cgtttatgtc tctggctgcc aattgcttaa
     1441 gcaagatcgg acggttaatg tgttttacac attttttccg tcaaacagtg aggcaggcca
     1501 tggagatgtt gtctggagcc gagatggtcg tccgatcgct tatcgatcag ggcgttaaac
     1561 aagtattcgg ttatcccgga ggcgcagtcc ttgatattta cgatgcattg cataccgtgg
     1621 gtggtattga tcatgtatta gttcgtcatg agcaggcggc ggtgcatatg gccgatggcc
     1681 tggcgcgtgc gaccggggaa gtcggcgtcg tgctggtaac gtcgggtcca ggggcgacca
     1741 atgcgattac cggcatcgcc accgcttata tggattccat tccattagtt gtcctttccg
     1801 ggcaggtagc gacctcgttg ataggttacg atgcctttca ggagtgcgac atggtgggga
     1861 tttcgcgacc ggtggttaaa cacagttttc tggttaagca aacggaagac attccgcagg
     1921 tgctgaaaaa ggctttctgg ctggcggcaa gtggtcgccc aggaccagtg gtcgttgatt
     1981 taccgaaaga tattcttaat ccggcgaaca aattacccta tgtctgccct gagtcggtca
     2041 gtatgcgttc ttacaatccc actactaccg gacataaagg gcaaattaag cgtgctctgc
     2101 aaacgctggt agcggcaaaa aaaccggttg tctacgtagg cggtggggca atcatggcgg
     2161 gctgccatca gcagttgaaa gaaacggtgg aggcgttgaa tctgcccgtt gtttcctcat
     2221 tgatgggaat gggggcgttt ccggcaacgc atcgtcaggc actgggcatg ctgggaatgc
     2281 acggtaccta cgaagccaat atgacgatgc ataacgcgga tgtgattttc gccgtcgggg
     2341 tacgatttga tgaccgaacg acgaacaatc tggcaaagta ctgcccaaat gccactgttc
     2401 tgcatatcga tattgatcct acttccattt ctaaaaccgt gactgcggat atcccgattg
     2461 tgggggatgc tcgccaggtc ctcgaacaaa tgcttgaact cctgtcgcaa gaatccgccc
     2521 atcaaccgct ggatgagatc cgcgactggt ggcagcagat tgaacagtgg cgcgctcttc
     2581 agtgcctgaa atatgacact cacagtgaaa agattaaacc gcaggcggtg atcgagactc
     2641 tttggcggtt gactaatggg gacgcttacg tgacgtccga tgtcgggcag caccagatgt
     2701 ttgctgcgct ttattatcca ttcgacaaac cgcgtcgctg gatcaattcc ggcggtctcg
     2761 gcacgatggg ttttggttta cctgcggcac tgggcgtcaa aatggcgttg ccagaagaaa
     2821 ccgtggtttg cgtcactggc gacggcagta ttcagatgaa catccaggaa ctgtctaccg
     2881 cgttgcaata cgagttgccc gtactggtgg tgaatctcaa taaccgctat ctggggatgg
     2941 tgaaacagtg gcaggacatg atctattccg gccgtcattc acaatcttat atgcaatcac
     3001 tacccgattt cgtccgtctg gcggaagcct atggtcacgt cgggatccag atctctcatc
     3061 cgcatgagct ggaaagcaaa cttagcgagg cgctggaaca ggtgcgcaat aatcgcctgg
     3121 tgtttgttga tgttaccgtc gatggcagtg agcacgtcta cccgatgcag attcgcgggg
     3181 gcggaatgga tgaaatgtgg ttaagcaaaa cggagagaac ctgattatgc gccggatatt
     3241 atcagtctta ctcgaaaatg aatcaggcgc gttatcccgc gtgattggcc ttttttccca
     3301 gcgaggctac aacattgaaa gcctgaccgt tgcgccaacc gacgatccga cattatcgcg
     3361 tatgaccatc cagaccgtgg gcgatgaaaa agtacttgag cagatcgaaa agcaattaca
     3421 caagctggtc tatgtcttgc gcgtgagtga gttggggcag ggcgcgcatg ttgagcggga
     3481 aatcatgctg gtgaaaattc aggccagcgg ttacgggcgt gacgaagtga aacgtaatac
     3541 ggaaatattc cgtgggcaaa ttatcgatgt caccccctcg ctttataccg ttcaattagc
     3601 aggcaccagc gataagcttg atgcattttt agcatcgatt cgcgatgtgg cgaaaattgt
     3661 ggaagttgct cgctctggtg tggtcggact ttcgcgcggc gataaaataa tgcgttgaga
     3721 atgatctcag tgcgcaattt gcagcccaac atgtcacgtt gggctttttt tgcgaaatca
     3781 gtgggaacct ggaataaaag cagttgccgc agttaatttt ctgcgcttag atgttaatga
     3841 atttaaccca taccagtaca atggctatgg tttttacatt ttacgcaagg ggcaattgtg
     3901 aaactggatg aaatcgctcg gctggcggga gtgtcgcgga ccactgcaag ctatgttatt
     3961 aacggcaaag cgaagcaata ccgtgtgagc gacaaaaccg ttgaaaaagt catggctgtg
     4021 gtgcgtgagc acaattacca cccgaacgcc gtggcagctg ggcttcgtgc tggacgcaca
     4081 cgttctattg gtcttgtgat ccccgatctg gagaacacca gctatacccg catcgctaac
     4141 tatcttgaac gccaggcgcg gcaacggggt tatcaactgc tgattgcctg ctcagaagat
     4201 cagccagaca acgaaatgcg ctgcattgag caccttttac agcgtcaggt tgatgccatt
     4261 attgtttcga cgtcgttgcc acctgagcat cctttttatc aacgctgggc taacgacccg
     4321 ttcccgattg tcgcgctgga ccgcgccctc gatcgtgaac atttcaccag cgtggttggt
     4381 gccgatcagg atgatgccga aatgctggcg gaagagttac gtaagtttcc cgccgagacg
     4441 gtgctttatc ttggcgcgct gccggagctt tctgtcagct tcctgcgtga acaaggtttc
     4501 cgtactgctt ggaaagatga tccgcgcgaa gtgcatttcc tgtatgccaa cagctatgag
     4561 cgggaggcgg ctgcccagtt attcgaaaaa tggctggaaa cgcatccgat gccgcaggcg
     4621 ctgttcacaa cgtcgtttgc gttgttgcaa ggagtgatgg atgtcacgct gcgtcgcgac
     4681 ggcaaactgc cttctgacct ggcaattgcc acctttggcg ataacgaact gctcgacttc
     4741 ttacagtgtc cggtgctggc agtggctcaa cgtcaccgcg atgtcgcaga gcgtgtgctg
     4801 gagattgtcc tggcaagcct ggacgaaccg cgtaagccaa aacctggttt aacgcgcatt
     4861 aaacgtaatc tctatcgccg cggcgtgctc agccgtagct aatccgcgaa caaaaatacg
     4921 cgccaggtga atttccctct ggcgcgtaga gtacgggact ggacat
//
LOCUS       DAEBXN010000212         4870 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.213, whole genome shotgun sequence.
ACCESSION   DAEBXN010000212 DAEBXN010000000
VERSION     DAEBXN010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4870
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.213"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..527
                     /locus_tag="KJL99_004200"
     CDS             <1..527
                     /locus_tag="KJL99_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBD4438707.1"
                     /translation="TTGRAEATILVDIAHQFWHIPHEKIWIEDQSTNCGENARFSITL
                     LNQAVERVHTAIVVQDPTMQRRTMATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVI
                     FINQLQGLWPVERYLSLLTGELPRLHDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKH
                     DAVLIEAMESRSLR"
     gene            724..2163
                     /gene="aldA"
                     /locus_tag="KJL99_004201"
     CDS             724..2163
                     /gene="aldA"
                     /locus_tag="KJL99_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HBD4438708.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLCKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNLLGEALQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(2205..3205)
                     /gene="gap"
                     /locus_tag="KJL99_004202"
                     /pseudo
     CDS             complement(2205..3205)
                     /gene="gap"
                     /locus_tag="KJL99_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
     gene            3394..3924
                     /gene="cybB"
                     /locus_tag="KJL99_004203"
     CDS             3394..3924
                     /gene="cybB"
                     /locus_tag="KJL99_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HBD4438709.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            4169..4342
                     /locus_tag="KJL99_004204"
     CDS             4169..4342
                     /locus_tag="KJL99_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438710.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(4414..4563)
                     /gene="hokB"
                     /locus_tag="KJL99_004205"
     CDS             complement(4414..4563)
                     /gene="hokB"
                     /locus_tag="KJL99_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HBD4438711.1"
                     /translation="MKRNPLVVCLLIICITILAFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            complement(4664..4804)
                     /locus_tag="KJL99_004206"
                     /pseudo
     CDS             complement(4664..4804)
                     /locus_tag="KJL99_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         1247 a         1120 c         1208 g         1295 t
ORIGIN      
        1 gcaccactgg cagagcagaa gcgaccatcc tggtggatat cgctcatcag ttctggcata
       61 ttccgcatga aaaaatctgg attgaagacc agtcaacaaa ctgcggtgaa aacgcacgct
      121 ttagcatcac gctattgaat caggccgtag aacgagttca tacggctatc gttgtacagg
      181 accccaccat gcagcggcgc acgatggcaa cgttccgccg tatgaccggg gacaatcccg
      241 atgcaccacg ctggttaagt tatcccggat tcgttcctca gttaggaaat aacgcagaca
      301 gtgtaatctt tattaatcag ttacaaggat tatggccggt tgagcgttat ctctcactac
      361 tcactggcga gctgccgcgt ttacacgatg atagcgatgg ctacggtccc cgcgggcgag
      421 attttatcgt tcacgttgat tttccggcag aagtcatcca tgcatggcaa acgctgaaac
      481 atgatgcggt gctcatcgag gcgatggaaa gtcgctcgtt acgttaaaaa ttgcccgttt
      541 gtgaaccact tgtttgcaaa cgggcatgac tcctgacttt tatttctgcc ttttattcct
      601 tttacacttg tttttatgaa gccctccaca gacttgtcct ttcccgatgc cgtctctctg
      661 atgattgatg ttaattaaca atatattcac cgaaaacaaa catataaatc acaagagtcg
      721 cccatgtcag tacccgttca acatcctatg tatatcgatg gacagtttgt tacctggcgt
      781 ggagacgcat ggattgatgt ggtaaaccct gctacagagg ctgtcatttc ccgcataccc
      841 gatggtcagg ccgaggatgc ccgtaaggca atcgatgcag cagaacgtgc acaaccagaa
      901 tgggaagcgt tgcctgctat tgaacgcgcc agttggttgt gcaaaatctc cgccgggatc
      961 cgcgaacgcg ccagtgaaat cagtgcgctg attgttgaag aagggggcaa gatccagcag
     1021 ctagctgaag tcgaagtggc ttttactgcc gactatatcg attacatggc ggagtgggca
     1081 cggcgttacg agggcgagat tattcaaagc gatcgtccgg gagaaaatat tcttctgttt
     1141 aaacgtgcgc ttggtgtgac taccggcatt ctgccgtgga actttccgtt cttcctcatt
     1201 gcccgcaaaa tggctcccgc tcttttgacc ggtaatacca tcgtcattaa acccagtgaa
     1261 tttacgccaa acaatgcgat tgcattcgcc aaaatcgtcg atgaaatagg ccttccgcgc
     1321 ggcgtgttta accttgtact ggggcgtggt gaaaccgttg ggcaagaact ggcgggtaac
     1381 ccaaaggtcg caatggtcag tatgacaggc agcgtctctg caggtgagaa gatcatggcg
     1441 actgcggcga aaaacatcac caaagtgtgt ctggaattgg gtggtaaagc accggctatc
     1501 gtaatggacg atgccgatct tgaactggca gtcaaagcca tcgttgattc acgcgtcatt
     1561 aatagtgggc aagtgtgtaa ctgtgcagaa cgtgtttatg tacagaaagg catttatgat
     1621 cagttcgtca atctgctggg tgaagcgttg caggcggttc aatttggtaa ccccgctgaa
     1681 cgcaacgaca ttgcgatggg gccgttgatt aacgccgcgg cgctggaaag ggttgagcaa
     1741 aaagtggcgc gcgcagtaga agaaggggcg agagtggcgt tgggtggcaa agcggtagag
     1801 gggaaaggat attattatcc gccgacattg ctgctggatg ttcgccagga aatgtcgatt
     1861 atgcatgagg aaacctttgg cccggtgctg ccagttgtcg catttgacac gctggaagat
     1921 gctatctcaa tggctaatga cagtgattac ggcctgacct catcaatcta tacccaaaat
     1981 ctgaacgtcg cgatgaaagc cattaaaggg ctgaagtttg gtgaaactta catcaaccgt
     2041 gaaaacttcg aagctatgca aggtttccac gccggatggc gtaaatccgg tattggcggc
     2101 gcagatggta aacatggcct gcatgaatat ctgcagaccc aggtggttta tttacagtct
     2161 taatgagtga aagaggcgga ggttttttcc tccgcctgtg cgcgtcagag tttagcgaat
     2221 ttttcgaggg tgcgaataag ctgcgtgacg aagccatatt cgttatcgta ccaggcgacc
     2281 gttttcacca gttgtaaatc gcccacggcg gtaatttccg tttgcgtggc atcaaacact
     2341 gaaccgaaat ggctgccaat gatatcggaa gagactattt cttcatcggt ataaccaaat
     2401 gactcgttat tggtggttgc ttgtttaagt gcgttattca cctcttcggc agtcactttt
     2461 ttccgagaat ggacaccagc tcagtgaccg aacctgtttt caccggcacg cgttgcgcat
     2521 gacctttcag tttgccgctc agttccggga tcaccagacc aatggctttt gccgcccccg
     2581 tagtgtgggg aatgatattt tctgccgctg cgcgtgaagc acgtaaatct ttaccacgcg
     2641 ggccatccac cagtgactgg gtgccagtat aggcatgaat ggtcgtcatc gtgccgactt
     2701 ctatcccgaa actgtcatgc aaggctttgg ccatcggcgc aaggcagtta gtggtgcatg
     2761 acgccacgga aacaatggtg tcgttgccat ccagagtgtc gtcattaacg ttataaacga
     2821 tggttttcat ttcaccggca ggagcggaaa tcaacacctt cttcgcacca gcatcaagat
     2881 gcgcctgcga tttatcggcg gaggtataaa agccagtaca ttcgacaatg atttctgccc
     2941 ctttcgcttt ccacggaata tttttagcct ctttttcggc gtaaaccgcg atacttttcc
     3001 catcaacgat aagtgaatct tccgtaaaat caacgctcca ggggaacgga ccatagtttg
     3061 aatcatgttt cagcagatag gcgagaattt ttggggaagt aagatcatta atagcgacaa
     3121 cgtctatgtt gcttttgact tcaagtaaac gacgcaacac cagtcgaccg atacgaccaa
     3181 aaccgttaat accaacttta ctcatggttt tctcctgtca ggaacgttcg gatgaaaatt
     3241 gatcctttcc aggcttagac caggattgtg ggatgggcaa tctccattct tacagtgaaa
     3301 cgtaacgtac tgaaaacggg tgaacaatat ttaatgaaat tttgagaaaa gcccgttatg
     3361 ttaacggaaa attatgttaa agcaggaaat gttatggaaa ataaatactc aaggttacaa
     3421 atcagcattc actggctggt ctttttgctg gttatcgcag cgtattgcgc aatggagttt
     3481 cgtggtttct tcccacgtag cgatcggccg ctcatcaaca tgattcatgt ttcctgtggc
     3541 atctcaattc tcgtgctgat ggtggttcgt cttttgttaa gactgaaata cccaaccccg
     3601 ccgattatac ctaagccgaa accgatgatg acgggactgg cgcatttggg acatttggtg
     3661 atttatctcc tgtttattgc gctgccagtg attggtttgg tgatgatgta taaccggggc
     3721 aacccgtggt ttgcgtttgg tttgacgatg ccttacgctt cagaggccaa tttcgaacgg
     3781 gttgatagct taaagtcgtg gcatgagacg ctggcgaatc tgggatattt tgtcatcggg
     3841 ttgcacgctg cggctgcact ggcacaccac tatttctgga aggacaacac acttctacgc
     3901 atgatgccgc gtaaacgttc ctgaaggata tttaaagaaa acgcctgtac taaaaccgac
     3961 ccgtggtaca ggcgaagaat acgggtctac atcggaagcg cctattatat ttatttgtat
     4021 gacaaataaa accataatcc ttgcccatac gtccatctgg cttattttta atcaattcac
     4081 ccgatctttg atctcatcaa cggtatcaaa ataaaccagc gtataacgtt caaatcgcac
     4141 ataagattcg ataaaccatg gaggttatat gaaaaaactg gcacttattt tgtttatggg
     4201 aacgcttgtt tccttttatg ccgatgccgg gcgcaaaccc tgttctggtt cgaaaggggg
     4261 gatctcacac tgtacggcag gcggcaaatt tgtctgtaat gatggttcta ttagtgcatc
     4321 gaaaaaaaca tgcactaact gaagtgtaaa aggggtgcca tgagaaaatg aatctgctag
     4381 tcagatgcgc ggggaaaatc cccgcgcttg cccttacctg gacgtgcagg ccatgagcgc
     4441 agcaacctcc ttatcaccgt cccggaaccg cagttcgtag agcgtttgtc gggtcaggag
     4501 tgtgaatgcc agaatcgtaa tgcagataat gagcagacac accaccagag ggttgcgctt
     4561 catagccttt ctccttgccg gatggcgggt aagaggctaa caatctgaat tgcttggttc
     4621 attcgttggc ctcggtttgt actgacccca aaaagttgga cagttaaaca cgaggcatat
     4681 aggtctgatt ccgatattca tttggagtca gaccttttaa tttcaggcta attcttctgc
     4741 tgttgtagta ttcaatatat tccgtaacag catccttcag ttcgcttata ttactgaact
     4801 cattagactg gccccctgaa tctccagaca accagtatca cttatttaag tgatagtctt
     4861 aatactagtt
//
LOCUS       DAEBXN010000213         4783 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.214, whole genome shotgun sequence.
ACCESSION   DAEBXN010000213 DAEBXN010000000
VERSION     DAEBXN010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4783
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.214"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(267..1124)
                     /gene="sitD"
                     /locus_tag="KJL99_004207"
     CDS             complement(267..1124)
                     /gene="sitD"
                     /locus_tag="KJL99_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HBD4438712.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(1121..1978)
                     /gene="sitC"
                     /locus_tag="KJL99_004208"
     CDS             complement(1121..1978)
                     /gene="sitC"
                     /locus_tag="KJL99_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HBD4438713.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(1975..2802)
                     /gene="sitB"
                     /locus_tag="KJL99_004209"
     CDS             complement(1975..2802)
                     /gene="sitB"
                     /locus_tag="KJL99_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HBD4438714.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(2802..3716)
                     /gene="sitA"
                     /locus_tag="KJL99_004210"
     CDS             complement(2802..3716)
                     /gene="sitA"
                     /locus_tag="KJL99_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HBD4438715.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            complement(4148..>4783)
                     /locus_tag="KJL99_004211"
     CDS             complement(4148..>4783)
                     /locus_tag="KJL99_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBD4438716.1"
                     /translation="PLSKTEFNALMESEKGQSQNLWKFAVYSGLRHGELAALAWEDVD
                     FEKGIVNVRRNLTILDMFGPPKTNAGIRTVALLQPALEALKEQYKLTGHHRKSEITFY
                     HREYGRTEKQKLHFVFMPRVCNEKQKPYYSVSSLGTRWNAAVKRAGIRRRNPYHTRHT
                     FACWLLTAGANPAFIASQMGHETAQMVYEIYGMWIDDMNDEQIAMLNARLS"
BASE COUNT         1268 a         1168 c         1129 g         1218 t
ORIGIN      
        1 gagctgaagg aagagctgaa tagtctgaag gatttgaatt tggttaggaa ttttatcgtg
       61 gaataacaga agctgcggca cgtcgtatgc aggaacgtgc cgcggttggc tggtaaactt
      121 tcgatagtgc gagtattgaa tgatttctag gcgttatcga ttttacgtat tttttgcatg
      181 atagaattcg tacctcctcc caacgacctt ccatggcttt ccggacttct gtaggctggc
      241 ggacgatgct gtcctcggat gttgtgctaa ttgggtgata acagatcctg tgtatgggcg
      301 tttccctttg tgcgtgcgtt gatgctggta acggtaaacg tcatgatgaa cacgaccgcg
      361 aataagacga caatcgtcgg agctggtgcg ctgtcgataa aaaaggacag ccagacacca
      421 gtcattgaca ctattaccga tatgccagtc gccagtagga gcgcaatatg aaagcgttgt
      481 gtcaggagta ctgcaatggc gccaggcgca atgagcagag aaatagagag aatgatacct
      541 actgctttca gcgtcgccac aatggtcagg gaaaccatgc acagcagccc ataatgcagc
      601 catctcgtat gcaaaccgct tacctgcgcc tgttgataat caaagctgaa cagcaaaaaa
      661 tcccgccatt ttacgctaat aacaagtgta accagcccgg caataatcac cgtctggatt
      721 atgtcgccga tggttatccc gagcatgtcg ccgaaaagaa tatggtcaag atgtacgtct
      781 ggtttaattg caatatacaa gatcagacct gcggcaaaca tgccagaaaa aacaatcccc
      841 ataactgtat cttgcttgat tcggctgttg tctttcaggt atcccgtcgc caccgcgcag
      901 aatactccgg cgacgaatgc ccctgtagcc agaggtaacc ccagaatcca ggtaagcacg
      961 atgccaggaa aaaccgcatg actcatcgcg tcacccatca gtgcccaacc tttcaggacc
     1021 agaaagaccg acagcagcgc acagggtatg gaaactacca gtgaaatcag cagtgcgtga
     1081 ctcataaagg tgaactgcag tggctccagt aataatgcca tcatgagcga tcctccagcg
     1141 ctttatgcgc acgccgacga tttgccaaaa gtccatgggt gggcgcaaat acaaatgcta
     1201 aaagaaacag cagtgtctgc gccaccacga tgattccacc tgtcgcgccg tccagaaagt
     1261 aactgaccca cgcgccgagg aaactggtta cactgccaat agttacagca attatgagca
     1321 ggcggggaaa acgatcggtt aacaaccatg ctgttgcacc gggagtcacc accagacaga
     1381 tcaccagaaa ggcaccaacc gtttgcagcg ccgcaacggt cgaaaccgac agcagggtga
     1441 agaagaggat ttttaatctg cccggatgta atccaatagc acgggcgtga ttctcgtcga
     1501 aaaaggtcac cataagatct ttccatttga aaaaaagcac gatgatcgag agaatgccaa
     1561 tgatagtgag ttgcaggata tccgccggat cgattgccag aatattgccc aggacgatgg
     1621 tctgaatgtt cacggatgtc gggttcaatg acaccataaa cagccccagg ccaaaaaagg
     1681 acgagaagat caggccgatg atggcatctt cttttaagcg ggtgcgttgg ttaagaaaca
     1741 gcatgctgcc cgctgccagt ccgccggaaa agaaggctcc cagagaaaac ggaagcccca
     1801 gcatatatgc ccccgcaaca cccgggacaa tagagtgcga cagcgcatcg ccaatcagcg
     1861 accagccttt tagcatcaga tagcatgaca ggaaagcgca gagaccgccg accattgccg
     1921 agacccacat cgcattgagc atatattcat agctgaaggg ttccagtagc acgttcatct
     1981 tgcttccctc tgcaccgctg acggtcgatg tgccacaaaa gggcgttcat cgtcagtaat
     2041 aatgctttct tccgagccgt taagtgtgac atggcgcagt acgccgctaa aagccagctc
     2101 caggttctcg gctgtaaaag tggtgtctgt cggcccgctt gccaggacgg tgcctttgac
     2161 catgaccgta taatcgcaaa atgtcgtgac tgagccgaga ttgtgcgttg agacaagcat
     2221 cgttttgcct tccgcgcgta actccctcaa caggctgatg attttcgctt cggttttcac
     2281 atcaacgccg gtaaaaggtt catccagcag gatcacatca ccctgctgtg caattgctct
     2341 cgccagaaag acgcgtttct tttgtccacc ggaaagctcg ccgatttgac gatggcgaaa
     2401 atccaccata tcgacacgtt ccagcgcatc ggtgacaatc tgacgatccc tctttttggc
     2461 tatacgcaac atacccatat gcccatagcg gcccatcatc accacatctt ccaccagtac
     2521 aggaaatgac cagtcaacct cttctgactg agggacgtag gcaaccaggt ttttctgtag
     2581 cgcctgtcgc gtgggaatac ccagaacaga tatctttccg ctggtcagac gcacaaatcc
     2641 cataatcgct ttaaacaacg tcgattttcc ggaaccgttt accccaacca gagcggcaat
     2701 agatccactt ggtactgtga aggatgcatc gcgcagtgct gtgtgcccgt tacgccaggt
     2761 gacagtaaca tcattaacga caatgcctgc agattgcatc attatttctc ccttttcccg
     2821 gctttaattc cctgtaccag cgtactggta gtaaccttaa gaaggtcgat atacgtaggt
     2881 accgggccgt tttctgtgct cagggaatcg acatagagca caccgccgta gtgtgcgccg
     2941 gtttcacgcg caacctgacg cgctggttta tcggaaatcg tactctcgct aaagactgcc
     3001 gggatatgat ttttcttaac tatatcaaca accttacgta cctgctgcgg tgttccttgt
     3061 tgatcggcat taatcggcca cagataaagc tcttttagcc ccaaatcgcg tgcgagataa
     3121 gaaaaagccc cttcactggt gaccatccat cgctgattct cagggagttc cgtaatctgc
     3181 ttacgcaggg gggcaagggt ttgggtaatc ttggctttat aagtatcggc attacgttgg
     3241 taggtttgtg catttgccgg atcgtatttt atcaacgcat cacgaatatt atcgacgtaa
     3301 atcagagcat tatctggcga catccatgca tggggattag gtttgccctc atagggtcct
     3361 tcggtgatcc ctactggcgt cacacccgaa gagacaatta cttctggaac cccattgaga
     3421 tgctggtaaa agcgttggaa ccacaattcc agattcatac cattggcgag aatcagttgt
     3481 gccccctgcg cacgtttaat atcgccaggg gtaggctgat actcatgaat ttctgcacca
     3541 ggcttggtta tggatgagac ttctgcagca tctccagcca cgtttttggc catatccgcg
     3601 atgatggtaa atgttgtaat gaccttgaat ttttcagttg cacttgcctg aaatgcgatc
     3661 gagcaggtga gggcgaggca ccccaagagc atggttactt tttttattga gtgcataatt
     3721 tagtacctat agtgagcaaa attagaacaa acatagcaac ggctatgttt tatagcataa
     3781 cctatacaaa atgaaaataa ttatcattaa cagctttgag gcctaaaaca gggattaggt
     3841 tgtttcctgc tggcgacatg atattgagga aaaaagtaga gcagctatta ggctttgaaa
     3901 tgactttatg aggaaaattg ttgatggcaa aaatggggca aaaaagagtg ggttatcgta
     3961 gcttattgtt gtcgctgata atgtttaaca tattgaaaaa taaataaaat acttatgagt
     4021 cagatagttg tgatttttgc ccttacttgt tcaggttgta ttgttctttc tttgtaattt
     4081 gttgattttc ttgcattatt tcagttctct ggtactaaat ggggcaaatt gggggcaaac
     4141 tttgcaacta cgataaccgc gcattcaaca tggctatctg ttcgtcgttc atgtcatcaa
     4201 tccacatacc gtaaatttca tacaccattt gcgcagtttc atgccccatc tggctggcta
     4261 taaatgccgg gttcgctcct gccgtcaaca gccagcaggc aaaagtatgt cgcgtatggt
     4321 acggattacg gcggcgaata ccagcacgtt ttactgctgc attccatctt gtacccaaac
     4381 tgcttaccga gtaataaggt ttctgttttt cgttacacac cctgggcatg aaaacaaaat
     4441 gcagtttttg tttttcagtt ctgccgtact cccgatgata aaaagtgatt tcgcttttgc
     4501 gatgatgccc ggtcagtttg tattgctcct tcagtgcttc aagagccggc tgcagtaatg
     4561 ctaccgtccg gatcccggca tttgtttttg ggggaccgaa catatcaagt atcgtcaggt
     4621 ttcttctgac attcacaatt cccttctcga aatccacatc ctcccacgcc agagctgcca
     4681 gttccccgtg acgaagcccg gagtaaacgg caaatttcca caagttttgg ctctgtcctt
     4741 tttcactttc cattaatgca ttgaattctg ttttagataa cgg
//
LOCUS       DAEBXN010000214         4707 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.216, whole genome shotgun sequence.
ACCESSION   DAEBXN010000214 DAEBXN010000000
VERSION     DAEBXN010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4707
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.216"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            31..2211
                     /locus_tag="KJL99_004212"
     CDS             31..2211
                     /locus_tag="KJL99_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HBD4438717.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQR
                     VIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTT
                     FKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(2352..3635)
                     /locus_tag="KJL99_004213"
     CDS             complement(2352..3635)
                     /locus_tag="KJL99_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HBD4438718.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEALNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEEKK"
     gene            complement(3770..4651)
                     /locus_tag="KJL99_004214"
     CDS             complement(3770..4651)
                     /locus_tag="KJL99_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBD4438719.1"
                     /translation="MTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMSKIKAI
                     RRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHITTNPV
                     AATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCEMKWSD
                     IVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTRREPLS
                     SGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHKSDTMA
                     SQYRDDRGREWDKIEIK"
BASE COUNT         1135 a         1146 c         1210 g         1216 t
ORIGIN      
        1 ttcacggtgc gcagatatct ggagcatttg atgatcaagt tatctgaaaa aggcgtgttt
       61 ctcgccagta ataacgaaat aattgccgaa gaacatttca ccggcgaaat taaaaaagaa
      121 gaagcaaaaa aaggcactat tgcctggtct attctctctt ctcataatac gtccggaaat
      181 atggataaac ttaaaattaa gtttgattca ttagcctctc acgatattac ctttgttggt
      241 attgtacaga ccgctaaagc gtccggaatg gaacgtttcc cgctgccgta tgtgctgacc
      301 aactgccata actcactctg cgccgtcggc ggcaccatta acggtgatga ccatgttttt
      361 ggtttatcgg cagctcagcg ttatggcggt atttttgtgc ctccgcatat tgcggtcatc
      421 catcaatata tgcgtgagat gatggctggc gggggcaaaa tgatcctcgg gtcagacagc
      481 cacacccgtt acggtgcatt agggacaatg gcagtcggtg agggtggcgg tgagttggta
      541 aaacagctgc ttaatgacac ctgggatatc gactatccgg gcgtggttgc ggtgcatctg
      601 accggaaaac ctgcgccgta tgtggggcca caggatgtgg cgctggctat cattggcgcg
      661 gtgttcaaaa acggttacgt caaaaacaaa gtcatggagt tcgttggacc gggcgttagc
      721 gcgctctcta ccgatttccg taacagcgtt gacgtgatga ccacggaaac cacctgttta
      781 agctccgtct ggcaaactga tgaagaagtg cataactggc tggcgctgca cggtcgcggt
      841 caggattact gccagcttaa ccctcaaccg atggcgtact acgatggctg catcagcgtt
      901 gatttaagcg ccatcaaacc aatgattgcg ctgccgttcc acccgagcaa cgtgtatgaa
      961 atcgacacac tgaaccagaa cctgaccgac attctgcgtg agattgaaat tgagtccgaa
     1021 cgcgtggcgc acggtaaagc caaactctcg ctgctggata aagtggaaaa tggtcgcctg
     1081 aaagtgcagc aggggattat cgcgggctgt tctggcggta actacgaaaa cgtcatcgcg
     1141 gcggcgaatg cactgcgcgg tcaatcctgt ggcaatgaca ccttctcgct ggcggtttac
     1201 ccgtcatcac agccggtgtt tatggatctc gccaaaaaag gtgtggtagc agatttgatt
     1261 ggcgcaggcg caatcatcag aaccgcattc tgtggcccat gctttggcgc gggcgatacg
     1321 ccaatcaaca acggtttgag tattcgccac accacgcgta acttcccgaa ccgcgaaggc
     1381 tctaagccag ccaatgggca gatgtcagcg gtggcgttga tggacgctcg ttctatcgct
     1441 gcgactgcgg caaacggtgg ctatttaacc tctgccagcg aacttgattg ctgggacaac
     1501 gtgccggagt acgccttcga tgtaacgccg tataaaaacc gtgtttatca gggctttgtg
     1561 aaaggggcaa ctcagcaacc gctgatttac ggaccgaaca ttaaagactg gccggaattg
     1621 ggtgcgctga ctgacaatat cgtcctgaaa gtgtgctcga agatcctcga cgaagtcacc
     1681 accaccgacg aactgattcc ttccggtgaa acctcttctt atcgttcaaa tccgattggt
     1741 ctggcgggga atgtcagcga gctgacagag gtgtttgcgc gcattaagca gattgctggt
     1801 caggagcata ttgatccgct gcaaactgaa attggcagca tggtctatgc ggtgaaacca
     1861 ggcgatggtt ctgcgcgtga acaggcggcg agctgccagc gtgtgattgg cggtctggcg
     1921 aatattgccg aggagtacgc gactaaacgc taccgttcta acgtcatcaa ctgggggatg
     1981 ttaccgctgc agatggcgga agtgccaacc tttgaagtgg gggattacat ttacatccct
     2041 ggcattaaag cggcgctgga taatccgggt acgacgttta aaggttatgt gatccatgaa
     2101 gatgcgccgg taacggaaat tacgctttac atggaaagcc tgacggctga agagcgcgag
     2161 attatcaagg cgggtagttt gattaacttc aataaaaacc gtcagatgta aaaagcgcca
     2221 tgtgaatgta ggtcgcattc ggcacttatt gtcggatgcg atgctggcgc atcttatccg
     2281 acctacaaat cgcatcgaac cgtaggccgg ataaggcgtt tacgccgcat ccggcaaata
     2341 gttaatagct cttacttctt ctcctctgca accactttgc tgcccacgcc gcggttattg
     2401 tattcccaca tgcggttgta gttagtgtca ttcagattgc gctgtatttc gtcgttatca
     2461 tctacgctgc cggtattacc cgcaaacgga cgattagaga tcaccgcatc ggcccacggt
     2521 ttagccgtgt taaaaccttc gttgatggcg ctatcacgga tcaccacctg accgttggta
     2581 ttagcatcaa catccagcga gcggcccagt tgcgccacgc catcaccgga agcattgaaa
     2641 cggctgttta cggcgaggaa accgtaatag atgttagaca gcgtagccgg tgcaaacaca
     2701 tatgcttctt gctgggtacg tgagttcacc acgcggaatt cggtgttatc gaacaccact
     2761 gcgccgcgac cagaaacgat atccacatcc ccttcaatat agctgttggt caccagcgta
     2821 cgcggctgac ggttggtttc cagacggttc tgcacaccgc tgttggtgac aaagaaggtg
     2881 ttctgacgac cgagaatgtt aacgttgtta atctgtactt ggtcaccgtc agtacgcagt
     2941 gctaccgccg gatggttacc tgcatctacg ctatcgccca gcgtgttttc gatggtcaga
     3001 ttttgcagtt gcagtccatt gttttgtgac cagaagaccg cagaacacag aacaccgata
     3061 ctgtcgctgc gtttgctctg gcagctatcg tacatatacc acgctggttt acctggcata
     3121 tatttgccgc gcgggttgac gtcgtgacgc cagtcggcag ggctcatgcc cccatcaagg
     3181 gaaagcccaa tcttcacatc aatcggtttt tcacctgtac cgtacagagt gattccacct
     3241 ggagcagcag ggacatacac cgttccctga tactcaccag gcatcacggc aatatactgg
     3301 cgcttgttgg tgcgcttgat aattgccgca tctaccgccg cctgaatcgt ggtatgcgtt
     3361 acaccttgag tacccgccgg gccgacaaca aagtcaggtt gcgcaggcag ggttatcggg
     3421 gacggattcc acgctgcagc acctggtgtc agggatgcaa aatagtgttg cgcatcgaaa
     3481 ttcagcgctt cttttgccga cagaatcggg cgagaagagg taccaggcgc agtttgatca
     3541 gaaggacgtt gatcgggtgg tgttgagcta caggcggtca gcgtcacgcc aaaagccaat
     3601 gccagcgcca gacgggaaac tgaaaatgtg ttcacaggtt gctccgggct atgaaataga
     3661 aaaatgaatc cgttgaagcc tgctttttta tactaagttg gcattataaa aaagcattgc
     3721 ttatcaattt gttgcaacga acaggtcatt atcagtcaaa ataaaatcat tatttgattt
     3781 caattttgtc ccactccctg cctctgtcat cacgatactg tgatgccatg gtgtccgact
     3841 tgtgcccgag aagatgttga gcaaacttat cgcttatctg cttctcatag agtcttgcag
     3901 acaaactgcg caactcgtga aaggtaggtg gatccccttc gaaggaaaga cctgatgctt
     3961 ttcgtgcgcg cataaaatac cttgatactg tgccggatga aagcggttca cgacgagtag
     4021 atgcaattat ggtttctccg ccaagaatct ctttgcattt atcaagtgtt tccttcattg
     4081 atatcccgag agcatcaaca tgcaatgttg tagggatggc aatttttacg cctgttttgc
     4141 tttgctcgac ataaagatat ccatctacga tatcagacca cttcatttcg cataaatcac
     4201 caactcgttg cccggtaaca acagccagtt ccattgcaag tctgagccaa catggtgatg
     4261 attctgctgc ttgataaatt ttcaggtatt cgtcagccgt aagtcttgat ctccttacct
     4321 ctgattttgc tgcgcgagtg gcagcgaccg ggtttgttgt tatatggcct tcagctattg
     4381 cctctcggaa tgcatcgctc agtgttgatc tgattaactt ggctgatgcc gccttgccct
     4441 cgtctatgta tccattgagc attgccgcaa tttcttttgt ggtgatgtct tcaagtggag
     4501 catcaggcag ccccctcctt attgctttaa ttttgctcat gtaatttatg agtgtcttct
     4561 gcttgattcc tctgctggcg aggatttttt cgtagcgatc aagccatgaa tgtaacgtaa
     4621 cagaattatc actgttgatt ctcgctgtca gaggcttgtg tttgtgtcct gaaaataact
     4681 caatgttggc ctgtatagct tcagtga
//
LOCUS       DAEBXN010000215         4636 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.217, whole genome shotgun sequence.
ACCESSION   DAEBXN010000215 DAEBXN010000000
VERSION     DAEBXN010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4636
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.217"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..311
                     /locus_tag="KJL99_004215"
     CDS             <1..311
                     /locus_tag="KJL99_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Involved in anaerobic NO protection; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HBD4438720.1"
                     /translation="LDNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQ
                     YYNQLHQLPLVALPLPFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA
                     "
     gene            468..1217
                     /locus_tag="KJL99_004216"
     CDS             468..1217
                     /locus_tag="KJL99_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HBD4438721.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            1239..1805
                     /gene="yieF"
                     /locus_tag="KJL99_004217"
                     /gene_synonym="chrR"
     CDS             1239..1805
                     /gene="yieF"
                     /locus_tag="KJL99_004217"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HBD4438722.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(1859..3196)
                     /gene="adeP"
                     /locus_tag="KJL99_004218"
     CDS             complement(1859..3196)
                     /gene="adeP"
                     /locus_tag="KJL99_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HBD4438723.1"
                     /translation="MSHQHTTQTSGQRMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPDYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            3361..4017
                     /gene="yieH"
                     /locus_tag="KJL99_004219"
     CDS             3361..4017
                     /gene="yieH"
                     /locus_tag="KJL99_004219"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HBD4438724.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSAIEGAGALLSAITAPMC
                     VVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVENCI
                     LVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWDITA
                     "
     gene            4084..4557
                     /locus_tag="KJL99_004220"
     CDS             4084..4557
                     /locus_tag="KJL99_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HBD4438725.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
BASE COUNT         1239 a         1162 c         1137 g         1098 t
ORIGIN      
        1 cactggacaa tgtgttacag gagctggggc gcgaacgcac gattgctatg agcctgccgg
       61 aattcgagca gtcactgttt atggcagcgc aacccgacaa tctgctactg gcgaccgcgc
      121 cgcgctactg tcagtactac aatcaactcc atcaactgcc gttggttgct cttcctctcc
      181 cgtttgacga aagccagcaa aaaaagctgg aagttccttt taccctgctg tggcataaac
      241 ggaacagcca taatccgaag atcgtctggt tacgggaaac cattaaaaac ctttacgcgt
      301 cgatggcata accgaatcgt atgaaacgcg acccaatttc acaataaaat gtaaaaaagt
      361 tgtaataagc ttgtctgaat cgaactttta gccgctttag tctgtccatc attccagtaa
      421 atgattactc ttgtattcat aatggaccat taagcatgga gcgaaaaatg gcgactcact
      481 ttgcccgagg gattttaacg gaaggacatc tgatttctgt tcgtctcccc tcccagtgtc
      541 atcaagaagc ccgaaacatt ccccctcatc gtcaaagccg ttttctggcg tccagaggtt
      601 tactcgcaga actgatgttc atgctgtatg gcattggcga attgccggaa atcgtcaccc
      661 tgccgaaagg taaaccggtt ttcagtgata aaaatttgcc ttcgttttct atttcctatg
      721 ccgggaatat ggttggcgtg gcgttaacaa ccgaaggtga atgtggcctc gatatggaac
      781 tacagcgtgc gacgcgcggg tttcatagcc cacacgcgcc cgataaccac accttttcca
      841 gcaatgaatc gctatggatc agtaaacaaa acgatcctaa cgaagcgcgg gcgcagctca
      901 ttacgctgcg ccgaagcgtg ctaaaactaa ccggtgatgt tttgaatgac gatccgcgcg
      961 atctgcagct gctgcccatt gccggacgcc tgaaatgtgc tcatgtaaat catgtagaag
     1021 cgttatgcga cgcggaggac gtgctggtgt ggtccgtggc ggtcacgccc gcgattgaaa
     1081 agctcagtgt ctgggagtta gatggcaaac acagctggaa aagcctgccg gatattcaca
     1141 gccgcgccaa caatcctacc agccggatga tgcgttttgc ccagctctct accgtgaagt
     1201 ctttttcgcc aaattgatag acaaccacag gagtcattat gtctgaaaaa ttgcaggtgg
     1261 ttacgttact ggggagcctg cgcaaaggct catttaatgg catggttgca cgtaccctgc
     1321 cgaaaattgc tccggcgagc atggaagtca atgcgttacc ttccattgcc gacattcccc
     1381 tgtatgacgc tgacgtacag caggaagaag gttttccagc aacggttgaa gctctggcgg
     1441 aacagatccg tcaggctgac ggtgtggtga tcgtcacgcc ggaatataac tactcggtac
     1501 cgggtgggct gaaaaatgcc atcgactggc tttcccgcct gccggatcaa ccgctggccg
     1561 gtaaaccggt gttgattcag accagctcaa tgggcgtgat tggcggcgcg cgctgtcagt
     1621 atcacctgcg ccagattctg gttttcctcg atgcaatggt gatgaacaag ccggaattta
     1681 tgggcggcgt gattcagaac aaagttgatc cgcaaaccgg agaagtgatt gatcagggta
     1741 cgctggacca cctgaccggg caattgaccg catttggtga gtttattcag cgagttaaga
     1801 tctaaataaa aaacccgcca gtaatcatgc atggcgggtt tttaatgcgc tatcgatttt
     1861 aatgagcgtc gataaataca atcttcagga taaacagcag cgcaacgatg attacgcacg
     1921 ggctaaggtc acgcagacgc ccggtaccaa tcttcatcac gcagtaggag ataaagccca
     1981 gcgcaatacc ttcggtaatc gaaaagctga acggcatcat cacggcggta ataaacgccg
     2041 gaacagattc agtaagatcc tgccagttca cgcgagcaag acttgaggtc atcaacacgc
     2101 caacgtagat caacgcgcca gctgcagcgt agtctggcac cattcccgcc agcggcgaca
     2161 gaaagataac cagcaggaac agcagaccaa caaccactgc cgtcagaccg gtacgaccgc
     2221 caaccgatac gccggaagag gactcaatat aagccgtaac ggaagaagta ccgataaacg
     2281 aaccggtcac ggaagagata ctgtcgacat acagcgcctg cttcatgcgc gggaatttcc
     2341 ccttctcatc cgccagacct gctttatcgg tcacgccaat cagcgtaccg gaggagtcaa
     2401 acaagttgac caacatgaaa gagaaaatca cccctgccag cccgaggtta aacgacccgg
     2461 ctaaatctac atgacccaca actgtcatta cgctcggcgg cgcagaaacg atgccattgt
     2521 agtgcacatc acccagcatc cagcccagca gcgtcgtcac cacgatagaa accagcaccg
     2581 ctgcgtgaat gttgcgcgag gccagaatag caatgatgaa aaagccgagg atacccagaa
     2641 gtacgctgtg agaagtcaga ttaccgatgc tcaccagcgt ttccgagtta gcgacaatca
     2701 cacctgcgtt tttcagcccc atcatgccga taaacaggcc gataccgctg gtaataccca
     2761 cacgcagact taccggaatg ttagcaatca tccagtaacg aacgcggaaa atggtcaaaa
     2821 gaagcagtcc gacagcgccc cagaagattg cgcccatacc gatctgccac ggcagcccca
     2881 ttgcctggac aaccacaaaa gcgaaaaagg cgttaagccc catcgcagga gccagcgcca
     2941 ccggaaggtt tgcaaacaat cccatcataa tgctaccgaa agcagcaatc agacaggttg
     3001 ttacgaagac ggcactggtg tccatgccag caacgcccag aatttgtggg ttaacaaata
     3061 cgatgtagac catcgtcagg aaggtggtga acccggcaat cacttcagtt cgtgccgtag
     3121 taccatgttc gcgcagttta aaaacacgtt ccagcatcct ctgaccagaa gtctgggtgg
     3181 tatgttgatg actcattatc tatttccgaa caaggaggga aaatccgtcg ctatcgtata
     3241 ccaaaatgcg acaataggcg cgtttgtgag agactttttt attggattta cttatacggc
     3301 aacgattgcg ttgcgcaaat cggctctacg aaaacgttaa actgattaaa aaggaaaggc
     3361 atgtcccgga tagaagcggt atttttcgac tgcgacggta cgctggtcga cagtgaagtc
     3421 atttgctccc gcgcatatgt aacgatgttt caggaatttg gtattacgct cgatcctgaa
     3481 gaggtattca aacgtttcaa aggtgtaaaa ctgtacgaaa ttatcgatat tgtttccctt
     3541 gaacatggtg ttacgttagc gaaaacagaa gctgaacacg tttaccgtgc agaagtcgct
     3601 cggctgttcg attcagccat cgaaggggct ggagcgctcc tgtcagcgat cactgcgcca
     3661 atgtgtgtgg tatctaacgg cccaaataac aaaatgcagc attctatggg caagctgaat
     3721 atgttgcact acttcccgga taaactgttc agcggctacg atattcagcg ctggaagcca
     3781 gacccggcgt taatgttcca tgcggcaaaa gcgatgaatg taaatgtaga aaactgcatt
     3841 ctggttgatg actcagttgc cggtgcgcaa tctggtatcg acgcaggtat ggaagtgttc
     3901 tacttctgcg ccgacccgca caataagccg atcgttcacc cgaaagtcac cacctttacc
     3961 catctttcgc agttaccgga actgtggaaa gcgcgtggtt gggatattac ggcatagttc
     4021 ttcacactcc cttcacttac cccgcttaaa ttggcgctca aaggtaagta aagggagttt
     4081 gatatgtctg tttcacgtcg ggtaatacat cacggacttt attttgcggt tttaggaccg
     4141 ttaattggtg ttctgtttct tgtcctctac atattcttcg caaaagaacc gctggttctt
     4201 ttggtaataa tacaggtact tccattgttt ctcttattgt cgattactac gggagctatt
     4261 cctgcattgt taaccggcgt gatggtcgcc tgtctgccgg aaaagatcgg gtcacagaaa
     4321 aattatcgtt gtctggctgg tggcataggt ggcgtcgtta tcaccgagat ctattgtgca
     4381 gttattgtac atattaaggg catggcttcc tcggagttgt ttgaaaacat tctttctggt
     4441 gacagtctcg ttgtccgcat cattcctgca ttgctggcag gtgtggtgat gagcagaatc
     4501 attacccgtc tgcccggatt ggatatttca tgtcctgaaa cagactcttt aagttaagcg
     4561 ggatacttta tctttgggct actcaaaagc agacaggatg ttactatgac tcaaaatatc
     4621 aggccgttac cccaat
//
LOCUS       DAEBXN010000216         4632 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.218, whole genome shotgun sequence.
ACCESSION   DAEBXN010000216 DAEBXN010000000
VERSION     DAEBXN010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4632
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.218"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..371
                     /gene="bglB"
                     /locus_tag="KJL99_004221"
     CDS             <1..371
                     /gene="bglB"
                     /locus_tag="KJL99_004221"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HBD4438726.1"
                     /translation="RVLLNTLWDRYQKPLFIVENGLGVKDSVEADGSIQDDYRIAYLN
                     DHLVQVNEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTR
                     KKSFGWYAEVIKTRGLSLKK"
     gene            457..2073
                     /gene="bglH"
                     /locus_tag="KJL99_004222"
     CDS             457..2073
                     /gene="bglH"
                     /locus_tag="KJL99_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HBD4438727.1"
                     /translation="MFRQNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMIDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTHTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIARNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNNIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRLEIRFYATYI
                     KALENELDGFTFEDNKDAQFAVGAQAEIWW"
     gene            2100..3275
                     /locus_tag="KJL99_004223"
     CDS             2100..3275
                     /locus_tag="KJL99_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HBD4438728.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSVVVGVPVPDNIHPMTKDEAGVWSWRTPILKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVAHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFSVRKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKFKIRH"
     gene            3277..3999
                     /locus_tag="KJL99_004224"
     CDS             3277..3999
                     /locus_tag="KJL99_004224"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBD4438729.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELAFG"
     gene            complement(4061..>4632)
                     /locus_tag="KJL99_004225"
     CDS             complement(4061..>4632)
                     /locus_tag="KJL99_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CbrC family protein"
                     /protein_id="HBD4438730.1"
                     /translation="GALTFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSGPFYCVD
                     EVEHLCPLCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDEMLKELV
                     ERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGIRNSDLA
                     KCLQKGGDCQGYLFRCLHCGKLRLWGDFS"
BASE COUNT         1284 a         1026 c         1131 g         1191 t
ORIGIN      
        1 tacgggttct gttaaatacg ctttgggatc gttatcaaaa accgttattt attgtcgaga
       61 atggattagg cgtaaaagac agcgttgaag cggatggttc gatacaggac gattatcgaa
      121 ttgcctattt aaacgatcac ctggtacagg taaatgaagc gattgccgat ggtgtggata
      181 ttatggggta caccagttgg gggccaattg atttagtcag tgcatctcat tcacaaatgt
      241 ctaagcgcta cggctttatt tatgtggatc gtgatgataa tggcgaagga agcctcacaa
      301 gaacgcgtaa gaaaagcttc ggatggtatg cagaagtgat caaaacgcgg gggctgtcat
      361 taaaaaaata accaataaag caccttaatt atcgtcgcat tcagaacagt ctggatgcga
      421 tacgttaatt ctttctttgc accataaagg gatattatgt ttagacaaaa tcttattacc
      481 tctgccatct tattaatggc accgttagcc ttttctgcac aatcattggc tgaatcatta
      541 acggtggaac aacgccttga gttattagaa aaagcgttaa gagaaacgca aagcgaactc
      601 aaaaagtata aagatgaaga gaagaaaaaa tatacgccag cgacggtgaa tcgtagcgta
      661 agtacgaatg atcaagggta tgccgccaat ccgttcccga ccagtagtgc cgcaaaacct
      721 gatgctgtac tggtcaaaaa tgaagagaaa aatgccagtg agacaggctc gatttattct
      781 tccatgactc tgaaagattt cagtaagttt gtgaaagatg aaattggctt tagttacaac
      841 ggctattacc gttctggttg ggggaccgcc tctcatggtt cacctaaatc atgggcgatt
      901 ggttctctgg gccgctttgg taacgaatac tccggctggt ttgatttgca gttaaaacaa
      961 cgtgtctaca acgaaaacgg caaacgggtt gatgccgttg tgatgataga tggtaacgtt
     1021 ggtcagcagt actctaccgg ctggtttggc gataatgccg gtggcgagaa ctttatgcag
     1081 ttctccgata tgtacgttac caccaaaggt ttcctgccct ttgcgccaga ggctgatttc
     1141 tgggtgggta aacacggtgc gccgaaaatt gaaatccaga tgctggactg gaaaacgcag
     1201 cgtactgatg ctgcagcggg tgtaggtctg gaaaactgga aagtcggtcc gggtaaaatt
     1261 gatatcgcgc tggttcgcga agatgtcgat gattacgatc gcagcctgca aaacaaacag
     1321 cagattaata cccatactat tgatttacgc tataaagata tcccgttatg ggataaagcg
     1381 accttaatgg tgagtggtcg ttatgtcacg gcaaacgaaa gcgcatcgga aaaagataat
     1441 caggataata acgggtatta tgactggaaa gatacctgga tgttcggcac atctttaacg
     1501 cagaaatttg ataaaggtgg cttcaacgaa ttctccttcc tggttgcgaa taactctatc
     1561 gccaggaact ttggccgtta tgctggcgca agtccattta ccacctttaa tggtcgttat
     1621 tatggtgatc acaccggcgg aacagcagtt cgtctgacct cgcagggcga agcctatatc
     1681 ggcgatcatt ttattgtggc taacgcgatt gtttactcct tcggtaacaa tatttatagc
     1741 tacgaaacag gcgcacactc tgatttcgaa tctattcgtg cggttgttcg cccggcctat
     1801 atttgggacc aatataacca gacaggtgtt gaactgggct atttcaccca gcaaaacaaa
     1861 gatgcgaata gtaataaatt taatgagtct ggttataaaa ccacgctctt ccataccttt
     1921 aaagtcaata ccagtatgtt gacctcgcgt ctggaaattc gtttctacgc cacgtatatc
     1981 aaagccctgg aaaacgaact ggatggcttc accttcgaag acaataaaga cgcccagttt
     2041 gctgtcggtg cccaggctga aatctggtgg taatggaact taatgaaagg aatattacga
     2101 tgaatataaa aattgctgct ttaacgctgg ctattgccag cggtatttct gctcagtggg
     2161 ccatagcagc ggatatgcca gccagcccgg cacccactat cccggtgaaa cagtatgtga
     2221 ctcaggtcaa tgccgataac agcgtgacct ttcgctactt tgcccctggg gcaaaaaatg
     2281 tctctgtagt ggtgggtgtt ccggttccgg acaatattca cccgatgacc aaagacgaag
     2341 caggagtctg gtcgtggcgc acacctatcc tgaaaggcaa cctgtacgag tattttttca
     2401 atgttgatgg tgtacgcagc attgatacag gcaccgcaat gactaagcct cagcgccagg
     2461 ttaactccag tatgattctg gtgccaggca gttatctgga tacgcgttct gttgcgcatg
     2521 gtgatttgat cgccataact taccactcca acgcattgca atctgaacgt cagatgtatg
     2581 tctggacccc gccaggatac accggcatgg gcgagccttt gccagtgctc tatttctatc
     2641 acggttttgg tgataccgga cgttccgcta tcgatcaggg gcgtatcccg caaatcatgg
     2701 ataacctgct ggctgaaggg aaaattaaac cgatgctggt ggtgatcccg gataccgaaa
     2761 ccgatgcgaa gggcattatt cccgaagatt tcgtgcctca ggaaagacgt aaagtctttt
     2821 atccgctgaa tgctaaagcg gcagatcgcg aactgatgaa cgatattatc ccgctgatta
     2881 gcaagcgttt tagtgtccgt aaagatgccg atggtcgcgc gctggcaggg ctttcacaag
     2941 gcgggtacca ggcgctggtt tccggaatga atcatctgga aagctttggc tggctggcca
     3001 cattcagtgg tgttaccacg acaaccgtac cggatgaagg tgtcgcggcc cggctgaacg
     3061 atccggcagc tattaaccag caactacgta attttactgt ggtcgttggt gataaagatg
     3121 tcgtaaccgg caaggatatc gccgggctga aaactgagct tgagcagaaa aaaattaagt
     3181 ttgattacca ggaatatccc ggcctgaacc atgaaatgga tgtctggcgg cctgcctatg
     3241 cagccttcgt acagaaattt aaaataaggc attaagatga aattaatcat taccgaagat
     3301 taccaggaaa tgagccgtgt cgcggcacac catctgcttg gttatatgtc gaagacgcgt
     3361 cgtgttaacc tggcaattac cgccggtagc acgcccaaag gcatgtatga atacctcacc
     3421 accctggtta aaggtaagcc ttggtacgat aactgctatt tctataattt cgatgaaatt
     3481 ccctttcgcg gcaaagaggg agaaggcgta acgattacca atctgcgtaa tctgtttttc
     3541 acccctgcgg ggatcaaaga agagaatatc cagaagctca ctattgataa ctaccgcgag
     3601 catgatcaga aactggcgcg tgaaggcggc ctggatttgg tggtgctggg attaggtgca
     3661 gatggtcatt tttgtggcaa tttgccgaat acgacccatt tccatgagca aaccgtggag
     3721 ttcccgatcc agggagagat ggttgatatt gtggcgcatg gtgagttggg cggcgacttt
     3781 tcgttagtgc ctgacagcta cgtgacgatg gggccgaaaa gcatcatggc ggcgaaaaac
     3841 ttgctgataa ttgtcagtgg cgcaggtaaa gcgcaggcac tgaaaaatgt gctgcaaggt
     3901 ccggttaccg aagatgttcc agcttccgtg ttgcagctgc atccttcgct gatggtaatc
     3961 gctgataaag ccgctgccgc agaattagcg ttcggttaag ctgaagaaat aaggccggat
     4021 gtatgaatta cgctccggcc tgttctcatt atttaaataa ctacgaaaaa tcaccccaca
     4081 gtctcagctt gccgcagtgg agacagcgga agagataacc ctgacaatca ccaccctttt
     4141 gcaggcattt agctagatca gaatttctaa taccgaaatt ctcacaatct tcttcaaggt
     4201 tggcaaattc atcgaggcga tcttttatat cattccagcc cacatagccg ataaaagcac
     4261 agaaatcgcc acaatgcgca agccagaatt cctgttgcca tccatgataa cctggcgtgc
     4321 gttcaaccaa ctctttcagc atttcatcag gatatgtgtt cttaataccg gcaaattcgt
     4381 cctcttcatc atactcaaat tcaacacctt ctatgctggc atcatcctga aaactacctg
     4441 caaacttttc tgcagcagaa ccgtccgcaa tacacaacgg acagagatgt tcaacttcat
     4501 caacgcaata aaagggaccc gagtaataaa ctgacgtctg ttgttcacag caatcgcact
     4561 ctacggtttt atcctgttca aatgcgcctg tttccagtgg cttgggatga tatttgaatg
     4621 taagcgcccc ga
//
LOCUS       DAEBXN010000217         4612 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.219, whole genome shotgun sequence.
ACCESSION   DAEBXN010000217 DAEBXN010000000
VERSION     DAEBXN010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4612
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.219"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            38..964
                     /gene="tauA"
                     /locus_tag="KJL99_004226"
     CDS             38..964
                     /gene="tauA"
                     /locus_tag="KJL99_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HBD4438731.1"
                     /translation="MAFIAFQAQAVNVTVAYQTSAEPAKVAQADNTFAKESGASVDWR
                     KFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKLGNSEALVVKKTI
                     SKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGLVEIVNLQPPAIIAAWQRGDIDG
                     AYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHPEVVKAFAKSAID
                     AQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQQQTAELTGPVNK
                     AIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            977..1744
                     /gene="tauB"
                     /locus_tag="KJL99_004227"
     CDS             977..1744
                     /gene="tauB"
                     /locus_tag="KJL99_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HBD4438732.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSDGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLRLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            1741..2568
                     /gene="tauC"
                     /locus_tag="KJL99_004228"
     CDS             1741..2568
                     /gene="tauC"
                     /locus_tag="KJL99_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HBD4438733.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            2565..3416
                     /gene="tauD"
                     /locus_tag="KJL99_004229"
     CDS             2565..3416
                     /gene="tauD"
                     /locus_tag="KJL99_004229"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HBD4438734.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSWLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(3522..4496)
                     /gene="hemB"
                     /locus_tag="KJL99_004230"
     CDS             complement(3522..4496)
                     /gene="hemB"
                     /locus_tag="KJL99_004230"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HBD4438735.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
BASE COUNT         1021 a         1204 c         1355 g         1032 t
ORIGIN      
        1 ataaattacc tagtcatata acacacttct tgccgcactg gcattcatcg cttttcaggc
       61 acaggcggtg aacgtcaccg tggcgtatca aacctcagcc gaaccggcga aagtggctca
      121 ggccgacaac acctttgcta aagagagcgg ggcgagcgtg gactggcgta agtttgacag
      181 cggagccagc atcgtgcggg cgctggcttc tggcgacgtg caaatcggca acctcggttc
      241 cagcccatta gcggttgcag ccagccaaca ggtgccgatt gaagtcttct tgctggcgtc
      301 aaaactgggt aactccgaag cgctggtggt aaagaaaact atcagcaaac ccgaagattt
      361 gattggtaaa cgcattgccg taccgtttat ctccaccacc cactacagcc tgctggcggc
      421 gctgaaacac tgggggatta aaccagggct agtggagatt gtgaacctgc agccgcccgc
      481 gattatcgcc gcatggcagc ggggtgatat tgatggtgct tatgtctggg caccggcggt
      541 taacgccctg gaaaaagacg gcaaggtgct gaccgattct gaacaggtcg ggcagtgggg
      601 tgcgccaacg ctggacgtct gggtggtgcg caaagatttt gccgagaaac atcctgaggt
      661 cgtgaaagcg ttcgccaaaa gcgccatcga tgcacagcaa ccgtacattg ctaaccctga
      721 cgcgtggctg aaacagccgg aaaacatcag caaactggcg cgtttaagcg gcgtgcctga
      781 aggtgacgtt ccggggctgg tgaagggaaa tacctatctg acgccgcagc aacaaacggc
      841 agaactgacc ggaccggtga acaaagcaat catcgacacc gcgcagtttt tgaaagagca
      901 gggcaaggtc ccggctgtag cgaatgatta cagccagtac gttacctcgc gcttcgtgca
      961 ataaaaggag gcgcagatgc tgcaaatctc tcatctctat gccgattatg gcggcaaacc
     1021 ggcactggaa gatatcaacc tgacgctgga aagcggcgag ctactggtgg tgctggggcc
     1081 gtccggctgc ggtaaaacca ccctgctgaa tctgattgcc ggttttgtgc cttatcagca
     1141 tggcagcatt caactggcgg gtaagcgtat tgagggaccg ggagcagagc gtggcgtagt
     1201 ttttcagaat gaagggctgc taccgtggcg caatgtacag gacaacgtgg cgttcggcct
     1261 gcaattggca ggtatagaga aaatgcagcg actggaaatc gcgcaccaga tgctgaaaaa
     1321 agtggggctg gaaggcgcag aaaaacgcta tatctggcag ctttccgatg gtcaacgtca
     1381 gcgggtgggg attgcccgcg cgctggcggc gaatccccag ctgttattac tcgacgaacc
     1441 gtttggtgcg ctggacgcct tcacccgcga ccagatgcaa actctgctgc tgaaactctg
     1501 gcaggagacg ggcaagcagg tgctgttgat tacccacgat atagaagaag cggtgtttat
     1561 ggcgactgaa ctggttctgc tttcatccgg ccctggccgt gtgctggagc ggctgcggct
     1621 caactttgct cgccgctttg ttgcgggaga gtcgagccgc agcatcaagt ccgatccaca
     1681 attcatcgcc atgcgcgaat atgttttaag ccgcgtattt gagcaacggg aggcgttctc
     1741 atgagtgtgc tcattaatga aaaactgcat tcgcggcggc tgaaatggcg ctggccgctc
     1801 tcgcgtcagg tgaccttaag cattggcacg ttagcggttt tactcactgt atggtggacg
     1861 gtggcggcgc tgcaactgat tagcccgctg tttttgccgc cgccgcaaca agtactggca
     1921 aaactgctca ccattgccgg accgcaggga tttatggacg ccacgctgtg gcagcatctg
     1981 gcagccagcc tgacgcgcat tgtgctggcg ctactcgcgg cggtggtgat cggtattccg
     2041 gtcgggatcg ccatggggct aagcccgacg gtgcgcggca ttctggatcc ggtaatcgaa
     2101 ctttatcgtc cggtgccacc gctggcttat ttgccgctga tggtgatctg gtttggtatt
     2161 ggtgaaacct cgaagatctt actgatctat ttagcgattt ttgcgccggt ggcgatgtcg
     2221 gcgctggcag gggtgaaaag cgcgcagcag gttcgcattc gtgccgccca gtcgctgggt
     2281 gccagccgtg cgcaggtgct gtggtttgtc attttgcccg gcgcgctgcc ggaaatcctc
     2341 accggattac gcattggtct gggtgtgggc tggtctacgc tggtagcggc ggagctgatt
     2401 gccgcgacac gcggtttagg atttatggtt cagtcagcgg gtgaatttct cgcgactgac
     2461 gtggtactgg cggggatcgc ggtgattgcg attatcgcct ttcttttaga actgggtctg
     2521 cgcgcgttac agcgccgtct gacgccctgg catggagaag tacaatgagt gaacgtctga
     2581 gcattacccc gctggggccg tatatcggcg cacaaatttc gggtgccgac ctgacgcgcc
     2641 cgttaagcga taatcagttt gaacagcttt accatgcggt gctgcgccat caggtggtat
     2701 ttctgcgcga tcaagctatt acgccgcagc agcaacgcgc gctggcccag cgttttggcg
     2761 aactgcatat tcaccctgtt taccctcatg ccgaaggggt tgacgagatc atcgtgctgg
     2821 atacccataa cgataatccg ccggataacg acaactggca taccgatgtg acatttattg
     2881 aaacgccacc cgcaggggcg attctggcag ctaaagagtt accttcgacc ggcggtgata
     2941 cgctctggac cagcggtatt gcggcctatg aggcgctctc tgttcccttc cgccagctgc
     3001 tgagctggct gcgtgcggag catgatttcc gtaaatcatt cccggaatac aaataccgca
     3061 aaaccgagga ggaacatcaa cgctggcgcg aggcggtcgc gaaaaatcct ccgttgctac
     3121 atccggtggt gcgaacgcat ccggtgagcg gtaaacaggc gctgtttgtg aatgaaggct
     3181 ttactacgcg aattgtcgat gtcagcgaga aagagagcga agccttgtta ggctttttat
     3241 tcgcccatat cactaaaccg gagtttcagg tgcgttggcg ctggcaaccg aatgatattg
     3301 cgatttggga taaccgcgtg actcagcact atgccaatgc cgattacctg ccacagcggc
     3361 ggattatgca tcgggcgacg atccttgggg ataaaccgtt ttatcgggcg gggtgatacg
     3421 agtgcggacg gttccctcgc ccccttgggg agagggttag ggtgaggggg cgttcactgt
     3481 actttcaaca ggttaactcc ccctttctga gaggaaaaaa attaacgcag aatcttcttc
     3541 tcagccaaat ccagcgcaaa gtagctgaaa atcagatccg cacccgcacg cttaattgaa
     3601 cctaagcttt cgagcacgac tttctcttca tctatagcac ccgccagcgc ggcgaactta
     3661 atcatcgcgt actcaccgct cacctgatac gcgccaatcg gcaattcagt acgttcacgc
     3721 agctcacgca cgatgtcgag gtacgctcca gcaggtttaa ccatcaggca gtctgcgccc
     3781 tgggcttcat ccagcagtga ctcacgaatc gcctcgcgac ggttcattgg gttcatctga
     3841 tagcttttgc ggtcgccttt taatgcgctt cctgcagctt cacggaacgg gccataaaag
     3901 gaagaggcga acttggtcga atacgacata atcgccgtat ctttaaagcc tgcagcgtcc
     3961 agcgcctgac gaatcgcctg tacctggccg tccatcgcgg cggaaggggc gatgaagtct
     4021 gcgcctgcag cagctgcaac cacggcttgc ttgcctaaat tttccagagt cgcgtcgttg
     4081 tcgacgccat gctcgcacag cacaccgcag tgaccgtgag aagtgtattc acagaaacaa
     4141 gtgtctgaca tgacgatcat ttctggcaca gtctgcttgc agatgcgcga catacgcgcc
     4201 accagtccat cttcccgcca ggcgtcgctg ccggtttcat cggtatggtg agagatgcca
     4261 aaagtcatta cggaacggat accggcgttg gcgatgcgtt caatttcgcg tgccagatgt
     4321 ttctctggaa tgcgcatcac gcctggcatg gcttcaacgg ctttgtagtc gtcaatttct
     4381 tcttcaacaa agatcggcaa caccaggtcg ttaaggctaa gtgttgtctc ttcaaacata
     4441 gcgcgcagcg caggagattt gcgcaggcga cgagggcgtt ggattaagtc tgtcatggtc
     4501 tgcctgatgt ttgtggaatt aaggtgcaca gtatacctga agcggggcag gagtgtttta
     4561 cgaaagttgt cgcgatgttg acaagaagag aatgaaagag agaccaggac at
//
LOCUS       DAEBXN010000218         4602 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.220, whole genome shotgun sequence.
ACCESSION   DAEBXN010000218 DAEBXN010000000
VERSION     DAEBXN010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4602
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.220"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(78..866)
                     /gene="phoH"
                     /locus_tag="KJL99_004231"
     CDS             complement(78..866)
                     /gene="phoH"
                     /locus_tag="KJL99_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HBD4438736.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLQAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDLLVRRLGASFMQYCLRPEIG
                     KVETAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(1473..2738)
                     /gene="efeB"
                     /locus_tag="KJL99_004232"
     CDS             complement(1473..2738)
                     /gene="efeB"
                     /locus_tag="KJL99_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HBD4438737.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALAGSCPVAHAQKTQSAPGTLS
                     PDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLSQG
                     GAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKMTR
                     FPNDSLDAALCHGDVLLQICANTQDTVNHALRDIIKHTPDLLSVRWKREGFISDHAAR
                     SKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQFR
                     VEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLANPR
                     TAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALEEY
                     VKPIGGGYFFSLPGVKDANDYLGRALLQV"
     gene            complement(2744..3871)
                     /gene="efeO"
                     /locus_tag="KJL99_004233"
     CDS             complement(2744..3871)
                     /gene="efeO"
                     /locus_tag="KJL99_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HBD4438738.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(3929..>4602)
                     /locus_tag="KJL99_004234"
     CDS             complement(3929..>4602)
                     /locus_tag="KJL99_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HBD4438739.1"
                     /translation="IFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNV
                     KVQLEQAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAM
                     LGLATAVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQ
                     EIAFDMSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGAT
                     ASRSA"
BASE COUNT         1081 a         1296 c         1109 g         1116 t
ORIGIN      
        1 ttcagaaaaa gcgctagtcc gggcataaac acccaaaaca aagcccggtt cgcccgggct
       61 ctgcaccgat aatacactta actgtatgca tgcagcgtac gttggcaaag tgccgaacgt
      121 acgcaatcct cttttccgaa ccggacgatc ccaaccattt catcttcttc gaaacgttcc
      181 agcgcgtcac ttaatccgga gcacacgccg cgaggcaaat cgcattgcgt gatatcaccg
      241 ttgacgataa ccgtcacgtt ctccccgagg cgggttaaaa acattttcat ttgcgcggca
      301 gtcacattct gcgcctcgtc aagaatgaca actgcatttt caaaggtacg tccacgcata
      361 taggcgaacg gcgcggtttc caccttccct atttccggtc gcaggcagta ctgcataaag
      421 gaagccccta agcgccggac cagcaagtcg tagaccgggc gaaaataggg agcaaacttt
      481 tctgcgatat caccaggtaa gaagccaaga tcttcatcgg cttgcagaac tggacgggtg
      541 acgataatcc tgtcgacatc cttatgtatc agggcctctg ccgcttttgc tgcgctgatc
      601 caggtttttc cgcacccggc ttcgcccgtg gcgaatatca gctgcttact ctcaatagcc
      661 tgcagatagt gcaattgcgc ttcatttcgc gcaaggatgg gcgaggtatc gcgactgtcg
      721 cgggccatac caatggcttc tacgccgctc atctgcacaa gcgaggtgac cgattcttct
      781 tcacgctgct tatggctgcg cgaatcccgt ctcagcacac gttttgcctc gcgacgagct
      841 ttgatcactg ctttttgtct tcccatggag agcaccttga gttgtttgta ttcatcacac
      901 gcgccgttgg cagcgcgatt atgcgcacga acatcagagg gttggcttcc ttgtaagcca
      961 tagtttgctt ttggataaaa tgccgaaaaa cggctacgca caccgtttgc ggcgtcggta
     1021 acacgagtaa agaaaggaag tgagaaaaat acagagtgac gaagttgcta ccggaggaga
     1081 aatctccgcg agtggtggag cgttgcttaa caaaacattg tccagaacag gacgttacca
     1141 tccgcgatct ccatagtgac tgactatcac tgccgggaac ttccgctgct acttaataag
     1201 tacaacagaa ctcgcattca ttgcaacaat atattcactt atatttaacg ataacaccca
     1261 tttcggtgac attaatttct actggaaaga tgacagagtg atgacagtga tggaaaaagc
     1321 tgtgtgcttt ccgcaggatt cgcagggctg gtaaaatgtg cctcgtgttt gccggattcg
     1381 gcgcgaacgc cttacccgac ctacaagtga ttaataattt gtgcacaaaa ctcatgcccg
     1441 ataaacctgc gcatcgggca tatttgcgtc gttcaaacct gcaatagcgc ccttcccaga
     1501 taatcgttcg cgtccttcac tcccggcagc gaaaaaaaat aaccgccgcc gataggttta
     1561 acgtattcct ccagcgcttc gccattgagc cttttttgta ctgtcaggaa gcctttttcc
     1621 agatcgtgtt ggtagcagac aaacagcaac cccatatcca gttgtccgga gttggtgacc
     1681 cccaacgaat aactgtagcc gcgacgcagc atcaggctgg actcactctc cgccgtgcgg
     1741 ggattcgcca gccggatatg gctgtccagc gcgatcacct tcccttccgg gtcgctggcg
     1801 taatcaggca catcatgctc atgctgcatt cccagcggcg caccggtttg cttatcacgg
     1861 ccaaaaatcg tctgctgttc tttcagcggc gttctgtccc aaaattccac tcgaaactga
     1921 atcaagcgta ctgcctgata gctgccaccg attgtccacg caggctcctg ctgatctgcc
     1981 gttacccaca ccactttttg catcaactta tcattctggc tatcgggatt ggcagtgccg
     2041 tctttgaaac ccagcaaatt aatcggcgtc tctttgcctt tactacgcgc cgcgtgatcg
     2101 gaaataaacc cttcccgctt ccagcgcaca ctgagcaaat ccggcgtgtg tttgatgata
     2161 tcgcgcagcg catggttcac cgtgtcctgg gtgttggcgc aaatctgtag caacacatca
     2221 ccatgacata acgccgcatc cagcgagtcg ttggggaaac gcgtcatctt ctgcagcttt
     2281 tttggcatct gtggcgcaag gccaaagcgc tcatcaaaca atgagtgacc caccgataac
     2341 gtgatggtga gattatcggg cgcaatgtag ccgccaagaa tgccggaatc gagtggtggc
     2401 aggcgcggat ttggcgtttc tggtgctgct ccgccctgac tcagaaaagc aaaacgctga
     2461 gtcaacaagc gaaacaaccg ctcaagatcg gctttatcgc tggcaagcac atcaaacgcc
     2521 accagcatca ttgcggcctg ttgtggcgtc aggatccctg cctgatgctc accataaaac
     2581 ggctgtttct cattgcgtgc atccggtgaa agcgtgcccg gtgcactttg cgttttttgt
     2641 gcatgagcga ccggacaact tcccgccagc gcaccaatcc ctttcagtaa acgtcggcgt
     2701 gacggttcat tcacgccgtt ttcatcttta tactgcataa cgcttaatcc agtcccagca
     2761 caccgcgaag ttgcgccaga tcttccgcca gcgcagtaat cggccctttc agcgcattcc
     2821 ggtcggcatc ggtcaatttg tcgtaggttt caaaaccgtc tttagtacgg tatttcgcca
     2881 gaatggtatc gaccttttta aagttggcat cgacttttgc caacagttcc gggttggctt
     2941 tttgcagttg tggacgcagc agatcgacaa ttttctgcga gccttcaacg ttagcctgga
     3001 aatcccacag atcggtgtgg ctgtagcgat cttcttcacc gctgattttg ctggctgcca
     3061 cttcctcaat cagtccggct gcgccgccga ccacttttga aggtgggaaa gccagttcac
     3121 tgatgcgttt ttgcaaatcg accacatcgg tataaagctg ctcagcgtac tgatccatcc
     3181 ctttggtggt gttgtcgcca aacaatgctt tttccagacg gtggaaacca gtgaattttg
     3241 ggtcggcggc tttttgctcg taatcatctt cacgggcgtc aatgctgcca tccagatcgg
     3301 agaacagttc agcaatcggt tcaatacgct catagtgctg gcgcgtcggc gcatacagtg
     3361 ctttcgcttt ttcgatatcg cctgctttaa tcgcgtcggt aaaggctttg gtgtcggtca
     3421 ccagctgcgt ggtttccgcc atgacatacg ctttatatgc agtaattgca ccaccaagac
     3481 ttaacagcgc atcactttgc gccgcatccg ccgttgcctc acctttgacg atcaacttcc
     3541 ctttcgggtt agtcagcaga ccgcaggtca tatcgtattc gccaggctgt aaattcgccg
     3601 tcattttctg gctaaaacca ggggcgatat tttcccgctc ttccaccacc atcacgcctt
     3661 tgaggatctc ccactccagc gccttctggc tatggttctg aataatgaac tgtgttttcc
     3721 cggcgttaac cgtaatggtc atcggttcgc actgcttatc cgtcacggtc actttgacct
     3781 gcggcacatc agcggcgtta gccataaaag cagaagaaaa cagcgcagcc acgctcaact
     3841 gcaatgcgtt acggcggaag ttaatggtca tgacgagact atccctttaa agtatgttgt
     3901 aactaagcaa gagtttgcgt cgtatttgtt acgcggagcg agacgctgtc gcccctgcgc
     3961 gtggtggcag agcaaatgcc accagcgccg ggatgagata aataaaccag acggcgactt
     4021 cgctgacgct cggtgcttcc tgatagccaa aaatcccttc catcagcgtg ccaaacagcg
     4081 agtgagttga gaggaccgcg ctcatatcga aggcgatttc ctgaaagtgg ttccacaatc
     4141 cggcttcatg aaatgcgcga atggcaccag ccgccagccc tgcggcgacg aagagaataa
     4201 acaggctggt ccatttaaaa aatgcaccaa gattgaggcg aataccgccc cagtagagca
     4261 ggaagccgag caccacggca gtagcaagac cgagcattgc acccagcggc ggccagatcc
     4321 cgacatcttg ttgaaatgcc gccagcagga aaaagaccga ctccagccct tcccttgcaa
     4381 cggcaaaaaa gaccatcatc accagcgccc agccatgatg atttccacgc tgcaatgcgc
     4441 tatcgactgc ctgttccagt tgcactttga cgttgcgcga cactttgcgc atccagaaaa
     4501 ccatccaggt aaggatcacc acggcgatca ccgccacgat accttcaaac agttcctgct
     4561 ctttttgcgg aaattcgccg gtggtttcgt taatgaagat cc
//
LOCUS       DAEBXN010000219         4373 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.221, whole genome shotgun sequence.
ACCESSION   DAEBXN010000219 DAEBXN010000000
VERSION     DAEBXN010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4373
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.221"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            158..599
                     /gene="tnpA"
                     /locus_tag="KJL99_004235"
                     /pseudo
     CDS             158..599
                     /gene="tnpA"
                     /locus_tag="KJL99_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317271.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(717..1661)
                     /gene="hypT"
                     /locus_tag="KJL99_004236"
                     /gene_synonym="qseD"
     CDS             complement(717..1661)
                     /gene="hypT"
                     /locus_tag="KJL99_004236"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001333359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HBD4438740.1"
                     /translation="MVMKNIETKWLYDFLTLEACRHFSQAAKERNLSQPAFSRRIKAL
                     EAAIGVVLFDRTTTPLQLTEEGKLFHSQTRSLLQQLECNLGELNGQSLLGVPNIKIAA
                     AHSLSLSVLPKLVHSLTAYGGEFVYHVEAIDVVQAVNTLREGKSDFIISFRDEDLMQS
                     PFCCLKLFESELYPVCAADAQGHPVFDITQPQVPLLNYTATSYMGRLVNRHLAEVGGI
                     TGRTIFISSMSELLKNMALNGYGIAWLPIWSIVDELQTKRLICLDAAKLTVPIQAYIY
                     RMNTRLNRTAENLWRILQEHMPDDLIQQISMEEPARRN"
     gene            2006..2725
                     /locus_tag="KJL99_004237"
     CDS             2006..2725
                     /locus_tag="KJL99_004237"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HBD4438741.1"
                     /translation="MKGLIGVLGGMGPAATVDLFNKFVTFTAAQCDQEHIPLIISSIP
                     DIPDRTDALMHHGRSPLPVMRDYMHKLEDAGAECIVIPCNTAHYWFKELKDACHIDIL
                     SIVETTINEVRACGKTRIGLLATNATLYMGLYQKGIESLGFTCVSPDADGQKKVMESI
                     YSLKAGNIAHAQKLMNEQAEILFSRGAEILVLGCTEIPVILAQAVKEQPSRYIDSTAS
                     LVRAGIKWYENRVGKHYLLTQ"
     gene            2791..4089
                     /locus_tag="KJL99_004238"
     CDS             2791..4089
                     /locus_tag="KJL99_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HBD4438742.1"
                     /translation="MVWLEIIVVLGAIFFGIRQGGIGIGLCGGLGLAILTLGFGLPMG
                     SPPVDVILIIMTVVVAASALQAAGGMDYLVRVAGNFMRRNPKYINIIAPIITWSMTIM
                     AGTGFIVFSTLPVIAEVAKESGIRPSRTLAGSVVASQVAISGSPISAAMAAMLTIMEV
                     NGVSFIQVMSVCLPTSFAAAMVAAFIASRQGCELQDDEVYLERLQKGLVQKYENNSSI
                     KPGATLSVGLFLLATVAIVILAAFPQLRPGFDIGKPMETRDIIIICMLSAACLMVVLC
                     KTATDAIILTSTFRAGMSSLAVILGIVTLGTTFIDAHLTEIKDIAGDILQAYPMLLAL
                     VLFGTCALLYSQGATTPLIIPLAVALDVPTWAILASYVAVTGVFVLPTYPTSLAAMEF
                     DTTGTTRVGKYVLNHPFMLPGLGGIIAGVALGFVIAPMII"
BASE COUNT         1096 a          953 c         1074 g         1250 t
ORIGIN      
        1 gaacaaattg tcgcccattg tgagtcatat tcgctctgac tttccagaac catacggact
       61 gcccgttgac ggacttcggg ggaaaaacga gtatttttag tcatcctgtt tacctctttc
      121 tcaggaagtt tagtctccag gattcccggg gcggttcata gcgcacaccc gatggaactg
      181 taaatatcac atagtttttt gcgccaaaat accgaagaca ggtgttctac agagagaagc
      241 gtagagcaac aggcagtatt ttgagaaagc tgtgtgagtg gaaaagcgta cggattctgg
      301 aagctgaatg ctgtgcagat catatccata tgcttgtgga gatcccgccc aaaatgagcg
      361 tatccggctt tatgggatat ctgaaaggga aaagcagtct gatgctttac gagcagtttg
      421 gtgatttgaa attcaaatac aggaacaggg agttctggtg caggggggac tacgtcgata
      481 cagtgggtaa gaacacggcg aagatacagg attacataaa gcaccagctt gaagaggata
      541 aaatgggaga gcagttatcg attccctgtc cgggcagccc gtttacggac cgtaagtaac
      601 gaagttggat gcaaatgtca gatcgtgtgc gcctgttagg gcgcggctgg taagagagcc
      661 ttataggcgc atttgaaaaa cctccggcta tgccggagga tatttattgc acgtcgtcaa
      721 ttgcggcgtg ctggctcttc cattgatatc tgctggatta aatcgtctgg catatgttcc
      781 tgtaaaatac gccagaggtt ctcggcggtt ctattcagtc gggtgttcat tcggtaaata
      841 taagcctgaa tggggactgt caatttagca gcatcaaggc atatcagacg tttggtctgt
      901 agttcatcga cgatcgacca gatcggtaac caggcaatgc cgtatccgtt gagcgccata
      961 ttctttaata actcgctcat cgaagagata aatatcgtgc gcccggtaat acctccaacc
     1021 tctgccagat gacgattgac caacctgccc atataagatg tggcggtata gttgagcaaa
     1081 ggaacctggg gctgagtgat atcgaataca gggtgcccct gtgcgtcggc ggcacatacc
     1141 gggtacaact cagattcaaa cagtttcagg cagcaaaaag gcgattgcat aagatcttca
     1201 tcgcgaaacg aaataataaa gtcactcttg ccttcgcgta acgtatttac ggcctggaca
     1261 acatcaatgg cttcaacatg atagacaaat tctccgccat aggcggtaag tgaatgcacc
     1321 agtttgggta atacgctcag ggatagtgag tgcgcggctg caattttaat gttgggaacg
     1381 ccgagtaaac tctggccgtt taactccccc aaattgcatt caagctgctg taataagctg
     1441 cgagtctggg aatgaaagag cttgccctct tcggttaatt gtaaaggggt ggtcgtgcga
     1501 tcgaataaaa caacgccgat cgctgcctcc agcgccttaa ttcgtcggct gaatgcgggt
     1561 tgcgaaagat tacgctcttt tgccgcctga gaaaaatggc gacaggcttc cagtgtcagg
     1621 aaatcgtata accattttgt ctcgatattc ttcattacca tggcacctgt attttcaatc
     1681 caaagacata ataaaacatc ccggcaataa aaataatgtt ctcaattagc acaacctggc
     1741 ctttatcatt cgttttgtgc ataacccctt catttccggt attgacgttg atatcacatt
     1801 tcgccaaaaa aacgaaattt ttccgtcaga taaccagaat acatcatcac tatggtgaat
     1861 aagcggaatg ttccatagct gcatcatgcg tgtcttgcat cgccttgtac aaccttgcat
     1921 ttctcatcgc ccatttcgac tgttaaaaaa gagagcagca gttatttacc gcgtattgtc
     1981 attaacgttt tgagaggcag gtattgtgaa aggtttaatt ggtgtactcg gtggaatggg
     2041 gccggcagct accgtcgacc tgtttaataa atttgtaact tttaccgctg cacaatgtga
     2101 tcaggaacat attcctttaa ttatttcttc gatcccggat attcctgacc gtaccgatgc
     2161 attaatgcat catgggcgtt caccattacc cgttatgcgt gattatatgc ataagctgga
     2221 agatgccggg gcagaatgta ttgttattcc ctgtaatacc gcgcattact ggttcaaaga
     2281 attaaaagat gcttgtcata ttgatatatt aagtatcgta gaaactacga tcaatgaagt
     2341 tcgcgcctgt ggtaaaaccc gaattggctt gttagccacc aatgccacgc tatatatggg
     2401 tttataccaa aaaggaattg agtcgctggg atttacctgt gtcagccccg atgccgacgg
     2461 tcagaaaaaa gtgatggaaa gtatttattc cttgaaagct ggcaatatag cgcacgcaca
     2521 aaagttgatg aatgagcagg ctgaaatatt attttcccgc ggtgcagaaa tccttgttct
     2581 tggttgtaca gaaattccgg ttattctggc gcaagccgtt aaagagcagc cttcccgcta
     2641 tattgactca acggcgtcac tcgttcgtgc cggaataaaa tggtacgaaa atcgtgtcgg
     2701 taaacattat cttttaaccc aataatcatt taaatcgcag ccagaaagtt attcgctttt
     2761 aactgaatta tatttataac ggagaacatt atggtttggc tggaaattat cgtagtactt
     2821 ggtgcaatat tttttggtat tcgccagggg ggaatcggta ttggtttatg tggcgggctt
     2881 gggcttgcca ttctgactct gggatttggt ctgcctatgg ggtcgccgcc agtcgacgtg
     2941 atcctgatta ttatgaccgt tgttgtggcg gcctcagcct tacaggccgc aggcggaatg
     3001 gattatctgg tacgcgtcgc tggtaatttt atgcggcgta acccgaaata tatcaacata
     3061 atcgcgccga ttatcacctg gtctatgacc attatggccg gtactgggtt tattgttttt
     3121 tccacgctgc cggtgattgc tgaagtggcg aaagagtccg gtatccgtcc ttcccgcacg
     3181 cttgccggtt cggttgtcgc ttcgcaggtt gcgatttccg gttcgccgat cagcgccgca
     3241 atggcggcta tgctcactat tatggaggta aacggtgtca gttttattca agtgatgtcg
     3301 gtatgtcttc ccacctcttt tgctgccgcg atggtcgccg cttttattgc ttcccgacag
     3361 ggatgtgagt tgcaggatga cgaggtctac cttgagcgcc tgcaaaaagg actggtgcaa
     3421 aaatacgaga ataatagcag catcaagcca ggtgcgacgc tctccgtcgg attgttcctg
     3481 ctggcgacgg tcgctattgt tattcttgct gccttccccc aactgcgtcc gggttttgat
     3541 atcggtaaac cgatggaaac acgcgatatc attattatct gtatgctctc tgccgcctgc
     3601 ctgatggtgg tgctgtgtaa gacggcgacc gatgccatta ttctcacctc cacattccgt
     3661 gcggggatga gttcgctggc ggtaattctc ggcattgtaa ccttaggcac cacttttatt
     3721 gatgcgcatt taactgagat taaagatatt gctggtgata ttttgcaggc gtatccgatg
     3781 ttgttggcgc tggttctttt tggcacttgc gcgctgctct actcgcaagg tgcgaccacg
     3841 ccgttaatta tcccgcttgc cgtcgcgctt gacgtgccca cctgggcaat tctggcgtcc
     3901 tatgtggccg tcaccggagt atttgtactg ccaacgtatc caacatcact ggctgccatg
     3961 gaatttgata ctaccggcac aactcgtgta ggcaaatatg tgctgaatca tccatttatg
     4021 ctgcctgggt tggggggaat tattgctgga gtggcattag gctttgtgat tgcgccaatg
     4081 attatttagc actatcaaac atcagtatcg atggtttata cacaataaag acagatatgg
     4141 cggaatatag tctgttgtaa gcgccaacaa agaatagggg aattccgcca tgtctgaaca
     4201 tcttcacaat atggtattaa gtgttgtcgt ttaatgattt atttcttggt tatgttgtgt
     4261 attagctgca tctatcgctt tttgatacct gaggtgtact ggcaatagcg gacactacca
     4321 tttgttcttt ttttaagcag tcatctgatg atatttttcc ctgaaggctg ccg
//
LOCUS       DAEBXN010000220         4209 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.222, whole genome shotgun sequence.
ACCESSION   DAEBXN010000220 DAEBXN010000000
VERSION     DAEBXN010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4209
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.222"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="KJL99_004239"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="KJL99_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBD4438743.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="KJL99_004240"
     tRNA            complement(324..399)
                     /locus_tag="KJL99_004240"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="KJL99_004241"
     tRNA            complement(406..480)
                     /locus_tag="KJL99_004241"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="KJL99_004242"
     tRNA            complement(597..681)
                     /locus_tag="KJL99_004242"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="KJL99_004243"
     tRNA            complement(690..765)
                     /locus_tag="KJL99_004243"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="KJL99_004244"
                     /gene_synonym="panK"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="KJL99_004244"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HBD4438744.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHVVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="KJL99_004245"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="KJL99_004245"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HBD4438745.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQFLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="KJL99_004246"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="KJL99_004246"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HBD4438746.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1089 a         1046 c          937 g         1137 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctagaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagtgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc tacacgatga agaaacagcc gaacggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctggcagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctttg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg cagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaaatc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa actgttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttccgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgttttct
     1861 gaaattccgc gaaggggctt ttacagaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgtg gtagaagagg tcagactacg caaataattt gcaggggagc gaatactccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagtaataa agccccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgacagat aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcaagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agctttcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gcccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggaactgat tcgtggagtc aataactggc
     2821 agcacggcta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 atgggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcgt ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     3301 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcattaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tctgcggttg catgctgcca ggcattgagt aattgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagat atgacgacag gaagagtttg tagaaacgca aaaaggcccg
     4201 actgggctt
//
LOCUS       DAEBXN010000221         4199 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.223, whole genome shotgun sequence.
ACCESSION   DAEBXN010000221 DAEBXN010000000
VERSION     DAEBXN010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4199
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.223"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            86..724
                     /locus_tag="KJL99_004247"
     CDS             86..724
                     /locus_tag="KJL99_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438747.1"
                     /translation="MSEQIKQDIDLIEILFHLKKKIRVILFIMAICMAMVLLFLYINK
                     DNTKVIYSLKINQTTPGILVSCDSNNNFACQTTMTEDVIQRITTFFQTSPDIKNREIR
                     LEWSGDKRDLPTAEEEISRVQASIIKWYASEYHNGRQVLDEIQTPSAINSELYTKMIY
                     LTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISVMFCLVKKW"
     gene            complement(996..1691)
                     /locus_tag="KJL99_004248"
     CDS             complement(996..1691)
                     /locus_tag="KJL99_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HBD4438748.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1684..3111)
                     /locus_tag="KJL99_004249"
     CDS             complement(1684..3111)
                     /locus_tag="KJL99_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HBD4438749.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVASAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(3122..3841)
                     /locus_tag="KJL99_004250"
     CDS             complement(3122..3841)
                     /locus_tag="KJL99_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HBD4438750.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAIKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT         1118 a         1085 c          869 g         1127 t
ORIGIN      
        1 ttttgctgat atttaacccg aacattcctg atacagacta ttaatacaac attatctgcg
       61 acagatttta ttttctggat atattatgag tgaacaaatc aaacaggata tcgatctgat
      121 tgagatttta tttcatctga agaaaaagat tcgtgttatc ctttttatta tggctatctg
      181 tatggcaatg gtgctgttgt ttctgtatat caataaagac aatacaaaag tgatttacag
      241 ccttaaaata aaccagacaa cgccaggtat acttgttagc tgtgatagca ataataattt
      301 tgcctgtcag accacaatga ctgaagatgt tattcagcga attactacat tttttcagac
      361 cagcccagat atcaagaaca gagaaataag gctggaatgg tcaggagata agagagattt
      421 accaactgct gaagaggaaa tatctcgcgt gcaggcgtct attatcaaat ggtatgcgtc
      481 agaataccat aatggcaggc aagttctcga tgagatacaa acgccttcag caattaacag
      541 tgagctttat acaaaaatga tatacctgac caggaactgg tcattgtatc cgaacggtga
      601 tggctgtgta actataagct caccagaaat aaaaaataaa taccctgctg ccatttgcct
      661 ggctctggga ttttttctaa gcattgtaat ttctgtaatg ttttgccttg tcaaaaaatg
      721 gtagatgaat accaacaaaa ctctgggcag taataaacag cattctgata gagagtgcct
      781 gtataaagct gaagtgacaa tgaccgggaa gggaacccgt tttcaggaat ggatgcctgt
      841 ctttttcact gtaaatatcc tggcattact aatgcctgga tatttacaga agagcagcat
      901 aaccatgacg gaacggggtt aagtcaggtt gtccttaacg ctattttcaa cctcgtatgc
      961 cttcttcagg tttatgttca gatctcatat ctctctcagc aatcctcaat aatcagatac
     1021 accgctttca ccggaccatg aaccccgacg actttgataa gctcaatatc cgccgttgag
     1081 ctgggaccac tgatgatgtt aatacatgac ggcattcgtt caccggcctg cgctttctga
     1141 tgcaattttt ctgcgagttg cgctacacgc ggcaggattg tgcttttacg caggataaaa
     1201 agagaagatt ccgggagcag gctcaatgaa cgcccgcgct cggcggcgga aaaaagaacc
     1261 acgcctcccg attcggttaa accatattca gcatacacga cacccacttt agcctgctct
     1321 gcctgcgaga tattctcggc acctttcgcc ggatcccaaa caacggcatt gcattcctgc
     1381 tgcaaacgtt cactaatccc caattcctcc agcctcgtgt caccgctaat cacgaccgac
     1441 tgatctccca gctctttaca cagacgtatt gcagcttctg ccgccttcgc ctcgctggtc
     1501 agctcacagc gcgtcaacat aacatcgctg gcaaactgaa taaacgcgtc acagcgctgc
     1561 tgttggttaa gttgggtaag ccgctcgtta gcatagttgt taagcggcgc atcttctgct
     1621 tgcggttcaa gtcgcagcgg gcgacccagt gcctgagcaa cgttattcaa aaattcgctc
     1681 cgattatcca ttctttttct cctgcgcctg atgtttctta aaccaactac ggaaactctc
     1741 tccgtcagct tcaggaagat cgcgtgcttc catccagtcg ctaatcgcgc caaatttgag
     1801 tggtgttttg ccgccattga taaaccagct tgccgcatga gcgccggcca tcatcccgac
     1861 tttccacaat cctggatgac tattggcata agcgaacatt tttatcgccc gttgctctgc
     1921 ttttgcggtg atcccttttt cagccatcac ccgacgatga cgcaaaatca gttttgacag
     1981 cggaatacgc accggacaca cgctgtcaca agcagtgcat aaagagcagg cgtagggtaa
     2041 atctttaaaa tctttatagc cgccaagtag cggagaaatc accgcaccaa ttggccctgg
     2101 ataaatagag ccatatccat gaccgccaat atggcgatat gccggacaag tattcataca
     2161 agccccgcag cgaatacagc gcagcacatc ccgaaattca gaggccagca cctcagaacg
     2221 cccgttatcg acaataacca gatgaaactc ttcaggacca tcaacgtgcc ccgcttcgcg
     2281 cggccctgtc agccaggtgt tgtatcccgt caaacgtgca ccaacggcac tgcgcgccag
     2341 catggtgatc aatacatcta cctcggcaaa cgtgggggca atacgctcca ttcccatcac
     2401 tgcaatatgc gttttaggca gcgtggtaca cattcgcgca ttaccttcat tggtcaccag
     2461 gcataccgaa ccggtctctg ccaccgcgaa attacagccg gtaataccta tttcagcact
     2521 gaggaaatct tcacggattt tttgccggat gaataaggtc atcgcttcag gcgtttccgg
     2581 cccctcatag cccagacgtt cgtgtagcac tcgacggatc tgatggcgat ctttatgaat
     2641 tgccgggacc acaacatgag atggcggatc ttgatccagc tggagaatat attcacccag
     2701 atcggtttca atcacctgaa tgccagcatc ctgcaacaca tgattgacac caatctcttc
     2761 ggtcaccatc gatttagatt tcaccacctt ccgggcattt ttgcgttggg caacctgtaa
     2821 aatgtagcgg gtagcgtctt ctttggtttt tgcaaaatag acgtgaccgc cgttttgcgt
     2881 cactttttct gagagctggt acagataagc gtcgagatta ctcagaacat gatcacgtat
     2941 ctgggcggcc cgatcgcgcc actcctccca gtgccccaat tcatcgacca ttttttgccg
     3001 atttgcccca atacgctgct gcgcgcttgc caccgctttg cgcatgatcg gatcttcaat
     3061 ttgctgacgg atgcgtgtct taaaatctgt attactggtt ttgatcgaca tctttatatc
     3121 ctcagcggct catcaacact tcagcaatat gcatcacttt gactttctgc ccttcccgtt
     3181 gtaatcgccc actgatgttc agcaggcaac tcacgtcagc accaattaaa tactcagggc
     3241 ggacttccat caggtgcaca accttttctt tcaccatctc gccggatatt tcggccattt
     3301 tgaccgagaa cgtgccgcca aatccgcagc aggtatcctg ttcagcaaag gtcaacagct
     3361 ccagtccacg cacatttttc agcagcgtaa gtggctcgtc cttcactccc agcttacggg
     3421 ccaggctaca agatgggtga tacacagctc tcccttgcaa actggcacct acatcgacta
     3481 cccctaattt attaacaata aaagaggtga gatcctgcat acgcgcggca accttttcgg
     3541 cacgtgatgc ccattcaggt tcatccgcca gatacgtcgg gtaacttttt atggcatagg
     3601 tgcaagagcc agccggtgaa ataatgggat cgtcgttatc ctccagtgcg gcgatcagat
     3661 ttttcatccc tggaatcgct tctttgatat aaccgctatt gatcgcaggc tgaccgcagc
     3721 atccctgttt ctccgggaaa tttacgcgac agccgagttt ttccagtagc agcacggagt
     3781 ctcgtgccat tcttgatttc agggcgtcac caatacaggt gacaaagaaa ttgacattca
     3841 caactaatac tccattactt catgcccatt tatgcccgat gtttcccccc caccggggga
     3901 tcaatcatca ataaatatgg cgttcttttt taattggcta gcaacatacc acccctcatt
     3961 tgtatgacaa atgataatct ttcatccaaa cttcaaatgc aataactaat tttatttctt
     4021 tcggcatcgc tcacatttta tggggaaatc gacgatcatt tttggcataa gaaatccatt
     4081 taaaaatggc tacatccctt atacctattc agagctactt attcaggggt tattgagata
     4141 tacactgatg gtgtacaaaa aaatcaccga caataatcac gtatcaagta atagtcgga
//
LOCUS       DAEBXN010000222         4189 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.224, whole genome shotgun sequence.
ACCESSION   DAEBXN010000222 DAEBXN010000000
VERSION     DAEBXN010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4189
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.224"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(351..998)
                     /gene="ychE"
                     /locus_tag="KJL99_004251"
     CDS             complement(351..998)
                     /gene="ychE"
                     /locus_tag="KJL99_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HBD4438751.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            1475..4150
                     /gene="adhE"
                     /locus_tag="KJL99_004252"
                     /gene_synonym="adhC"
     CDS             1475..4150
                     /gene="adhE"
                     /locus_tag="KJL99_004252"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBD4438752.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEDETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         1169 a         1021 c          961 g         1038 t
ORIGIN      
        1 ttcaattcaa tcaaatagag attggctaag catgtctatc ttttctgatt acagttccag
       61 tagtgaaatg cacaataatt taacaattga ttattattta gcactttcct ctaccaaagg
      121 cagtggaatt actaatatca tttcaattat tcttcagcaa gcccaggatt atgatgtggc
      181 gaaaataaca tgatggtgag gagaagcagt taaggaaatc cttataacta actgtaattt
      241 gagaagaata tttttaccta ttccttttgc ttttgataac tgcgcgggaa caaagtaaaa
      301 ataatgacga agtaaacgga gcatggcagc tccgtttcat ttgaaaggaa ttaattaagc
      361 aggccgggga aaatcccctt aataccagta acgataaatt caatccccaa tgccatcagc
      421 aatagcccca taatacgcgt aatcacgttg atgccggtct ggcgtaaaac ccgtaccagc
      481 cacggtgcca tgcggaacaa tccccagcaa cataaagcga acaatgcaat agccacaaag
      541 aaaccaaaca gatagctaat gctgtgataa cgcgtacccc agacgatggt agaactgatc
      601 gcccctggcc ccgccatcaa cggcaacgcc aatggcacca caccaatact ttcacgtacc
      661 gcggtttctg atttttcttg cttgttctgt ttatcctcgc caagcttgcc gctgatcatc
      721 gacatcgcta tcgtcaccac caggatgccc ccggcgatac ggaacgaatc aattgatata
      781 ccaaaaagtt gtagaatcgt gtcgccgaga aaaagcgaga tccacaagat aatggccaca
      841 gacaggttgg ctgtaaggtt agttttgttt cgcgctgccg ctgtctgata actggtcatg
      901 ctgataaaga cgggaataat ccctaccggg ttgaccagcg caaataaccc gatgaaaaat
      961 ttgaagtaaa cgggaaaatc aaaaagggtc tgaatcacgg ttcgctccga agcaaaagcc
     1021 ggataatgtt agccataaat aaggttgaaa agacgcgctg acaatacgcc ttttgacagc
     1081 atttttcacc tcctaactac ttaaaattgc tatcattcgt tattgttatc tagttgtgca
     1141 aaacatgcta atgtagccac caaatcatac tacaatttat taactgttag ctataatggc
     1201 gaaaagcgat gctgaaaggt gtcagctttg caaaaatttg atttggatca cgtaatcagt
     1261 acccagaagt gagtaatctt gcttacgcca cctggaagtg acgcattaga gataataact
     1321 ctaatgttta aactctttta gtaaatcaca gtgagtgtga gcgcgagtaa gcttttgatt
     1381 ttcattggtt aagcaaatca tcaccgcact gactatactc tcgtattcga gcagatgatt
     1441 tactaaaaaa gtttaacatt atcaggagag cattatggct gttactaatg tcgctgaact
     1501 taacgcactc gtagagcgtg taaaaaaagc ccagcgtgaa tatgccagtt tcactcaaga
     1561 gcaagtagac aaaatcttcc gcgccgccgc tctggctgct gcagatgctc gaatcccact
     1621 cgcgaaaatg gccgttgccg aatccggcat gggtatcgtc gaagataaag tgatcaaaaa
     1681 ccactttgct tctgaatata tctacaacgc ctataaagat gaaaaaacct gtggtgttct
     1741 gtctgaagac gacacttttg gtaccatcac tatcgctgaa ccaatcggta ttatttgcgg
     1801 tatcgttccg accactaacc cgacttcaac tgctatcttc aaatcgctga tcagcctgaa
     1861 gacccgtaac gccattatct tctccccgca cccacgtgca aaagatgcaa ccaacaaagc
     1921 ggctgatatc gttctgcagg ctgctatcgc tgccggtgct ccgaaagatc tgatcggctg
     1981 gatcgatcaa ccttctgttg aactgtctaa cgcactgatg caccacccag acatcaacct
     2041 gatcctcgcg actggtggtc cgggcatggt taaagccgca tacagctccg gtaaaccagc
     2101 tatcggtgta ggcgcgggta acactccagt tgttattgat gaaactgctg atatcaaacg
     2161 tgcagttgca tctgtactga tgtccaaaac cttcgacaac ggcgtaatct gtgcttctga
     2221 acagtctgtt gttgttgttg actctgttta tgacgcagta cgtgaacgtt tcgcaaccca
     2281 cggcggctat ctgttgcagg gtaaagagct gaaagctgtt caggatgtta tcctgaaaaa
     2341 tggtgcgctg aacgcggcta tcgttggtca gccagcctat aaaattgctg aactggcagg
     2401 cttctctgta ccagaaaaca ccaagattct gatcggtgaa gtgaccgttg ttgatgaaag
     2461 cgaaccgttc gcacatgaaa aactgtcccc gactctggca atgtaccgcg ctaaagattt
     2521 cgaagacgcg gtagaaaaag cagagaaact ggttgctatg ggcggtatcg gtcatacctc
     2581 ttgcctgtac actgaccagg ataaccaacc ggctcgcgtt tcttacttcg gtcagaaaat
     2641 gaaaacagct cgtatcctga ttaacacccc ggcttctcag ggtggtatcg gtgacctgta
     2701 taacttcaaa ctcgcacctt ccctgactct gggttgtggt tcctggggtg gtaactccat
     2761 ctctgaaaac gttggtccga aacacctgat caacaagaaa accgttgcta agcgagctga
     2821 aaacatgttg tggcacaaac ttccgaaatc tatctacttc cgccgtggct ccctgccaat
     2881 cgcgctggat gaagtgatta ctgatggcca caaacgtgcg ctcatcgtga ctgaccgctt
     2941 cctgttcaac aatggttatg ctgatcagat cacttccgta ctgaaagcag caggcgttga
     3001 aactgaagtc ttcttcgaag tagaagcgga cccgaccctg agcatcgttc gtaaaggtgc
     3061 agaactggca aactccttca aaccagacgt gattatcgcg ctgggtggtg gttccccgat
     3121 ggacgccgcg aagatcatgt gggttatgta cgaacatccg gaaactcact tcgaagagct
     3181 ggcgctgcgc tttatggata tccgtaaacg tatctacaag ttcccgaaaa tgggcgtgaa
     3241 agcgaaaatg atcgctgtca ccaccacttc tggtacaggt tctgaagtca ctccgtttgc
     3301 ggttgtaact gacgacgcta ctggtcagaa atacccgctg gcggactatg cgctgacccc
     3361 ggatatggcg attgtcgacg ccaacctggt tatggacatg ccgaagtccc tgtgtgcttt
     3421 cggtggtctg gacgcagtaa ctcacgccat ggaagcttat gtttctgtac tggcatctga
     3481 gttctctgat ggtcaggctc tgcaggcact gaaactgctg aaagaatatc tgccagcgtc
     3541 ctaccacgaa gggtctaaaa atccggtagc gcgtgaacgt gttcacagtg cagcaactat
     3601 cgcgggtatc gcatttgcga acgccttcct gggtgtatgt cactcaatgg cgcacaaact
     3661 gggttcccag ttccatattc cgcacggtct ggcaaacgcc ctgctgattt gtaacgttat
     3721 tcgctacaat gcgaacgaca acccgaccaa gcagactgca ttcagccagt atgaccgtcc
     3781 gcaggctcgc cgtcgttatg ctgaaattgc cgaccacctg ggtctgagcg caccgggcga
     3841 ccgtactgct gctaagatcg agaaactgct ggcatggctg gaaacgctga aagctgaact
     3901 gggtattccg aaatctatcc gtgaagctgg cgttcaggaa gcagacttcc tggcgaacgt
     3961 ggataaactg tctgaagatg cattcgatga ccagtgcacc ggcgctaacc cgcgttaccc
     4021 gctgatctcc gagctgaaac agattctgct ggatacctac tacggtcgtg attatgtaga
     4081 agatgaaact gcagcgaaaa aagaagccgc tccggctaaa gctgagaaaa aagcgaaaaa
     4141 atccgcttaa tcagtagcgc tgtctggcaa tataaacggc cccttctgg
//
LOCUS       DAEBXN010000223         4182 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.225, whole genome shotgun sequence.
ACCESSION   DAEBXN010000223 DAEBXN010000000
VERSION     DAEBXN010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4182
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.225"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(<1..186)
                     /locus_tag="KJL99_004253"
     CDS             complement(<1..186)
                     /locus_tag="KJL99_004253"
                     /inference="COORDINATES: protein motif:HMM:NF017243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HBD4438753.1"
                     /translation="MRHFIYQDEKSHKFWTVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAEKAELKLIAEKVKK"
     gene            complement(327..2360)
                     /gene="metG"
                     /locus_tag="KJL99_004254"
     CDS             complement(327..2360)
                     /gene="metG"
                     /locus_tag="KJL99_004254"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HBD4438754.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPELYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            2492..3601
                     /gene="apbC"
                     /locus_tag="KJL99_004255"
     CDS             2492..3601
                     /gene="apbC"
                     /locus_tag="KJL99_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HBD4438755.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMAGKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            3863..4144
                     /locus_tag="KJL99_004256"
     CDS             3863..4144
                     /locus_tag="KJL99_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HBD4438756.1"
                     /translation="MNKYWLSGIIFLAYGLASPAVSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
BASE COUNT         1016 a         1044 c         1062 g         1060 t
ORIGIN      
        1 cttcttcacc ttctccgcaa tcagcttcag ttccgctttt tccgccgccg cagcatctga
       61 aaaacttttt atctgacttt gccctttggt gccaactttt ccccaactga tatgcaactc
      121 gtttccctgt tgctccaccg tccagaattt atgtgatttt tcgtcctgat agataaagtg
      181 tctcattctg catccctata attttatcca gttgaatgca gatgctacca gtatttatac
      241 gggttagaga gagacaaaat cgcaagaaaa caactttagg aatttataaa gaaaaggcgc
      301 tgtcgatgca gcgccttgaa gggggattat ttcacctgat gacccggttt agcaccggca
      361 tccgggctta gcaggaaaat atctttcccg ccaggaccgg cagccatcac catgccttca
      421 gagataccga agcgcatttt acgtggtgcc aggttagcca ccataatggt gtgacgacca
      481 atcagtgcct gcggatccgg gtaagcagaa cgaataccgg agaagacatt gcgtttttca
      541 ccgccgagat ccagcgtcag gcgcagcagt ttgtcagaac cttcaacaaa ctctgcgttt
      601 tcgatcagcg ccacgcgcag gtcaactttg gcgaagtcgt caaaggtgat ggtttcctga
      661 atcggatcat ctgccagtgg gccagtaact ggcgcggtag tggctttcac ttcttcttta
      721 gaggcttcca ccagagcttc aacctgtttc atatcgatgc ggttatacag cgccttgaac
      781 ggattcactt tgtggcccag cagcggttgc tggataccat cccaggtcag ttccgtattg
      841 aggaaggctt ctgcacgctc ggtcagtttc ggcagtacag gcttcaggta agtcatcagc
      901 acgcggaaca ggttgatgcc cattgagcaa atcgcctgca ggtcagcatc gcgaccttcc
      961 tgttttgcga ctacccacgg agcctgctcg tcgacatagc ggttagccag gtcagccagt
     1021 gccatgattt cgcgcactgc tttaccaaat tcacggcttt cccacgcttc accaatcact
     1081 tcagcggcat cggtaaaggt tttgtacagc tcagggtcag ccagctcgct tgccagcaca
     1141 ccgtcaaaac gcttgttgat aaagcccgca ttacgggagg ccaggttaac cactttgttc
     1201 acgatatcgg cattcacacg ctgaacgaaa tcttccaggt tgagatcgat atcatcaatg
     1261 cgcgaagaga gtttcgcagt gtagtagtaa cgcaggctgt cagcgtcaaa atgattcagc
     1321 caggtgctgg ctttaataaa ggtgccgcga gacttggaca tcttcgcgcc gttcaccgtc
     1381 acatagccat gaacaaacag gttggtcggc ttgcggaagt tgctgccttc cagcatggca
     1441 ggccagaaca ggctgtggaa gtaaacgata tctttaccga tgaagtggta cagctcggcg
     1501 gtggagtctt tcttccagta ttcatcgaag cttacgctgt cgccgcgctt gtcgcacaga
     1561 ttcttgaaag aacccatgta gccaatcggt gcgtccagcc agacgtagaa atatttgccc
     1621 ggtgcgttcg gaatttcaaa accaaagtat ggcgcgtcgc gggagatatc ccactgttgc
     1681 aggccggatt caaaccactc ctgcattttg tttgccacct gctcctgcaa cgcaccgctg
     1741 cgggtccatg cctgcagcat ttcgctgaaa gagggcagat caaagaagaa gtgttcagaa
     1801 tcacgcatca ctggtgtagc accagaaacc accgatttcg gctcgatcaa ttcagtcggg
     1861 ctgtaggtcg cgccgcagac ttcgcagtta tcgccgtatt gatccgggga tttacatttc
     1921 gggcaggtgc ctttcacaaa acggtccggc aggaacatgc ctttttccgg atcgtacagc
     1981 tgagagatgg tgcggttttt aataaaaccg ttttctttca ggcgagagta gataagttct
     2041 gacaactggc ggttctcttc gctgtgcgtc gagtgatagt tgtcatagct gatgttaaag
     2101 cctgcgaaat cagtctgatg ctcctgactc atttcgccaa tcatctgctc cggggtgata
     2161 ccgagctgct gagctttcag catgatcggt gtaccgtggg catcgtcggc gcagatgaag
     2221 ttgacctcgt ggccgcgcat tcgctggtaa cggacccaga catcagcctg gatgtgctcc
     2281 agcatatggc cgaggtggat tgagccgtta gcgtacggca gtgcgcacgt caccagaatt
     2341 ttcttcgcga cttgagtcat agtaggcatt acttcttaat agtgaaaagg ggttagatag
     2401 taccaaatgg gaaaatgtta agtaagtact tctctatatt cttcaggtaa actatcagct
     2461 gatggtttat ctatacaaaa aaggagtcgg gatgaacgaa caatcccagg ccaaatcacc
     2521 agaagccttg cgcgcaatgg tcgccgggac actggccaat tttcagcacc caaccctgaa
     2581 gcataacctc accacgctga aagcgttgca ccatgtcgcc tggatggacg acacgttgca
     2641 tgtggaactg gttatgccgt ttgtctggca tagcgcgttc gaagagttaa aagagcaatg
     2701 tagcgccgaa ctgctgcgca tcaccggggc gaaagctatc gactggaaac tgtcgcataa
     2761 catagccact ctgaaacgcg tgaagaatca gccgggaatt aacggtgtga agaacattat
     2821 cgcggtgagt tccggtaaag gcggcgtggg taaatcgtcc acggcggtaa acctggcgct
     2881 ggcactggcg gcggaagggg cgaaagtcgg cattctggac gccgatatct acggcccatc
     2941 aattcccacc atgctgggcg cagaaaatca gcgtccaact tcgccggacg gtacccacat
     3001 ggcaccgatt atgtctcatg gcctggcaac caattcaatt ggttatctgg tcactgacga
     3061 caatgcgatg gtgtggcgtg gaccgatggc gggcaaggcg ttaatgcaga tgttgcagga
     3121 aaccttgtgg ccggatctcg actatctggt gcttgatatg ccgccaggca ccggtgacat
     3181 tcaattgacg ctggcgcaaa acattccagt aactggtgca gtcgtggtca ccaccccgca
     3241 ggacatcgcg ttgatcgatg cgaaaaaagg cattgtgatg ttcgagaaag tcgaggttcc
     3301 ggtgctcggt attgtcgaga acatgagtgt gcatatttgc agtaactgtg gtcaccatga
     3361 accgattttc ggtaccggtg gtgcagagaa actggctgaa aaataccata cacagctgct
     3421 ggggcagatg ccgttacaca tctccttacg tgaggatctc gataaaggca cgccaacggt
     3481 tatcagccgt ccggagagcg aatttaccgc tatctaccgc cagctggctg accgcgttgc
     3541 agctcagctc tactggcagg gtgaagtcat tcctggcgag atttccttcc gcgcggtcta
     3601 acgcctatta accattctgg ttattcttca tacaccgcag agatgttata ttgatgcggt
     3661 gtatttgttt ttgccgcatt aatgaacact tatttaatac tgcgaatatt ctgcggacct
     3721 tcgtccgaaa ctctacaaag tcgagtgatc gtacaggcaa tgcttcatta tcatccttcg
     3781 tgctgtgtag tggatgtaca gctatagttt gttatgcact cattctatcc aggcataacc
     3841 tttactacag ggataacgtt caatgaataa gtattggctg tcaggaatta tttttcttgc
     3901 ctatggattg gcttctcccg cagtctcttc agagactgcc acattaacga ttaatggcag
     3961 gatcagtccc ccaacatgca gtatggctat ggttaatagt caaccacaac agcattgtgg
     4021 tcagttaacg tataatgttg acacccgtca tcaggtttct tcgcccgtca aaggtgttac
     4081 gacagaagtg gttgttgcag gtagcgatag caaacgtcga atcgtgttaa atcgctacga
     4141 ttaatttggc aacctgattt taattgcatt tagaaagacg aa
//
LOCUS       DAEBXN010000224         4168 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.226, whole genome shotgun sequence.
ACCESSION   DAEBXN010000224 DAEBXN010000000
VERSION     DAEBXN010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4168
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.226"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(<1..334)
                     /gene="flhe"
                     /locus_tag="KJL99_004257"
     CDS             complement(<1..334)
                     /gene="flhe"
                     /locus_tag="KJL99_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HBD4438757.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     ARQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGG"
     gene            complement(334..2411)
                     /gene="flhA"
                     /locus_tag="KJL99_004258"
                     /pseudo
     CDS             complement(334..2411)
                     /gene="flhA"
                     /locus_tag="KJL99_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(2404..3551)
                     /gene="flhB"
                     /locus_tag="KJL99_004259"
                     /pseudo
     CDS             complement(2404..3551)
                     /gene="flhB"
                     /locus_tag="KJL99_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
     gene            3787..>4168
                     /locus_tag="KJL99_004260"
     CDS             3787..>4168
                     /locus_tag="KJL99_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBD4438758.1"
                     /translation="MEWQQLLNCNRRKDNLKKTNIAFLHPLASGRQEIERDYDRLLFA
                     APTRRLADKTQVFPLDQNDSIRTRLTHSHEVANFARGIGMRLAFEMKEHIFGEIPTGI
                     VVERDVPALLAAIGLAHDLGNPPFG"
BASE COUNT         1038 a         1208 c         1021 g          901 t
ORIGIN      
        1 caccgcctgg cacttcccag ataaatcgca acggttctgc tgccgctata ccggaaaagg
       61 ccacggtggt accgctctgc ccctctaatt cgacacaacg agattgcgaa cacaagcgaa
      121 cccgcagtcc tgcaggtgtc gggccgataa gctgataacg ccacgccacc agcgtcatca
      181 atcctgaagc aggttgtcgc gcagaaagag gtgcagacga catcgactca ccgcgatgat
      241 ttagcgtaat tccgacacta cttgcctgcc acatcccctc cccggcggct tgcaccagca
      301 gcggaaacaa taatatcgct aataaggttc tcatcattta ccgccaattg tcgccgtcat
      361 gcggatatgt cggttatcag acagttccag attcgacagg accactaact gcggcaagct
      421 gcggcgcagg aagcgagaca ataatggtcg cagcgcgtgg ttcaccaaca atactggcgg
      481 cgcacccagc atctcctgac gggatagagc ttcctgagtt tgcgccagta aacgatccgc
      541 cagccctggc tccagtcctc ccccgccctg cagcgcctgt agtaacaaac gttccagcgg
      601 tgtatcgagg ccaataacat ggacttcatc tttgccagga aaccactgct gggtaatcgc
      661 ccgtcccaac gccacgcgca cgacggcgct taattcatgt ggatcgcttt ggatgggcgc
      721 atgttccgcc agcgtttcga gaatggtgcg catatcgcga atcggtactt tttcatcgag
      781 gagattttgc agcactttat gcagtgtggt gagcgtgacg acgccaggaa cgagatcttc
      841 cgtcagcttt ggcatctcct gggcgacgcg atccaacagc tgttgcgcct cctgacgacc
      901 aaacagctct gcggcatgct ggctaatgag gtggttaaga tgcgttgcta ccaccgtgct
      961 ggcctcaacc actgtgtacc cctgaatctg cgcctgttct tttagcgcac tttcaatcca
     1021 gatagcattc aggccaaatg ccggatcgac ggtcgcctca ccaggtaacg tcccggcagc
     1081 ggttccaggg ttaatcgcca gccagcgccc cggataagca tcaccactgc caatctccac
     1141 gcctttcatc aaaatgcgat agcgggcagg ttgcagatcc atattgtcgc gaatgtgcac
     1201 cactggcggc agaaatccca tctcctgggc aaatttcttg cggatactgc gtatacggcc
     1261 caacaactca ccatcctgct ggaaatcgac catcgggatc agtcgataac ccacttccat
     1321 tcccagagaa tcttccagtt gtacatcgtt ccacgtcgct tcgacaacgg tattattctc
     1381 tgccattttt accggtttgg gttcggcagg cgctttttgt tcgcgtccgc gtatccacca
     1441 ggccagcccc agcaatccgg cagtgaacag caaaaatacc aggttcggca ttccaggcac
     1501 caggccgagt aaaccgagca cggcggcgct taacaacata acgcttgggt tactgaaaag
     1561 ctgattcacc atctgctcgc caacatcctg atcggtgctg acacgcgtaa cgatgccccc
     1621 cgcggcggta gaaatcacca gcgccggaat ttgtgccacc agaccgtcgc caatggtcaa
     1681 tagcgtataa ctttccgccg cgtgtcccat gctcatgcca gttgcagcac gccgaccagc
     1741 aacccgccga caacgttaat gaccatgatg aggatcccgg caatggcatc gccgcgaaca
     1801 aacttacttg ccccgtccat tgagccgtaa aaatcggctt cctgagtcac ttcggagcgg
     1861 cgttttttcg cctcatcttc accaatcaat ccagcgttaa ggtcggcgtc aatcgccatc
     1921 tgcttacccg gcataccatc gagaacaaag cgcgcaccca cttctgctat acgcccggca
     1981 cctttggtaa tgaccataaa gttgatgatc acgagaatga caaacaccac gataccgata
     2041 gcgaaattgc caccaacgag gaagtgaccg aacgcttcga ccaccttccc agccgccgcc
     2101 gcgccggtat gcccttccat caaaatgata cgggttgaag ccacgttaag tgccagacgc
     2161 aacaacatag taaacaacag aatggtcgga aacgcagcaa actcaagcgt gcgctgggta
     2221 aacatcgcca ccagcaacac catgatcgac aaggcaatat tgaaggtaaa caacaggtcg
     2281 agtatgaatg cgggcagtgg cagcaccatc atcgacaaga tcaacaggat caaaatcggt
     2341 ccggcaagga tctgccatta tgtcgatttc aggtttgcgg gcaggcgcag catcgcggcc
     2401 agattactca tgggtcggtt tctcgttaat aaaatccagg gctgccggca ccggaagatg
     2461 agtaggtgta cagggcgctg tccaccagcc agacgccagc gtttcagttg ccagacccag
     2521 gccagcactt ccgccaccgc ggcgtacagt tgacccggga tttgttgacc aatctccgca
     2581 tgtcgataca gcgctcgcgc cagcggcggc gcttcaagcg tcgggacgtt attttcagcg
     2641 ccaatttcac gaatgcgcag cgcgaccagc cctgcacctt tagcgaccac tttcggtgcg
     2701 ctcattttgt tttcgtcata ctgcaacgct accgaatagt gggtcgggtt attgacaatg
     2761 acatccgctt tcggcacatc ggccatcatc cgacgccgtg cggcagctcg ctgcatctga
     2821 cagatccgtc ctttaacatg ggggtcgcct tcgctttgtt tgaactcatc acgaatatcc
     2881 tgccgcgaca ttcgcagctt tttcaggtgg ctgaagattt ggaaaaagac gtcaaatccc
     2941 accattggaa tgacaccaag caccaccagc agtgcgcata gccctaccaa atccatcgca
     3001 ttacccatgg cggtaatcgg tgactcggcc attaagcgca tcatctgcgg ccagtgatgc
     3061 cagagaaaaa accccgtcac gctgccaacc aggatggttt tcaaaattgc cttaagcaac
     3121 tccgcgctag tctgtgccga gaatatccgt ttaatgcccg gtagcgggtt gagtttggaa
     3181 aacttcggct gcaaggattt gccgctaaat accagccccc ccagcataac cggagaaatg
     3241 agcgccacca gcaccacgcc gctaatcagc ggtagcagcg ccagcatggc ttctctgatc
     3301 agcagaataa tttgcccgag gatcagattc gggtctttga taatactgtg atcaaaatgc
     3361 agcccagcgg agagcatgcc cgacaatcga cgggccagcg acacaccgcc aaaccagata
     3421 acactaacgc ccaccagcaa aatcagcagt gaggtcagtt cacgggaacg cgggatttgc
     3481 ccctcttccc gcgctttttc tagtcggtga ggtgtggggg cttctgtttt gtcgtcgctc
     3541 tcgtcagaca cgtcgccaat cctggaaaga gtattaagcg taaatgatgc cagagcgcaa
     3601 agcgttcaat ggtttaagta aggggcaaaa caggcgggat ttagggcgtt tatgcaaaaa
     3661 taggttcagt tgcagaacgt tttgcgaacc attttccagt tctgatattt agccatttag
     3721 cctatagaat cgcactccgc ctctctgtta gtttgctaat aattaaatta ttacaaaggt
     3781 gatcaaatgg aatggcaaca attgcttaac tgcaaccgtc ggaaagataa tctaaaaaaa
     3841 accaacatag catttttaca tccccttgct agcggaagac aagagatcga gcgggattac
     3901 gaccgattac tttttgctgc accaactcgt cggcttgcgg ataaaacaca agttttcccg
     3961 ttagatcaaa atgacagcat acggacgagg ctgactcatt ctcacgaagt agcaaacttt
     4021 gctcgcggga taggaatgag gctcgctttc gagatgaagg agcacatctt tggcgaaata
     4081 cccacgggga ttgtcgttga acgcgacgtt cctgctctgt tagcagcgat cggactggca
     4141 cacgatctcg gtaatccacc atttgggc
//
LOCUS       DAEBXN010000225         4115 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.227, whole genome shotgun sequence.
ACCESSION   DAEBXN010000225 DAEBXN010000000
VERSION     DAEBXN010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4115
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.227"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(42..690)
                     /locus_tag="KJL99_004261"
                     /pseudo
     CDS             complement(42..690)
                     /locus_tag="KJL99_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164328.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            755..1906
                     /locus_tag="KJL99_004262"
                     /pseudo
     CDS             755..1906
                     /locus_tag="KJL99_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1936..2364)
                     /locus_tag="KJL99_004263"
                     /pseudo
     CDS             complement(1936..2364)
                     /locus_tag="KJL99_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
     gene            complement(2879..3922)
                     /locus_tag="KJL99_004264"
     CDS             complement(2879..3922)
                     /locus_tag="KJL99_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBD4438759.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
BASE COUNT         1146 a          899 c          956 g         1114 t
ORIGIN      
        1 ccggggcggt tcaagtagat acatacgcag tcagagtaca cttacttttt attatttcct
       61 gcaatggcga ttacggctat tgacagtaat gaagttatta gtgaaccaag acctaaagta
      121 gttccgggac caacaccgtt gtcgcttacg gttactgaca ggaaactgat tgtaccagcg
      181 cttattgttt gagtaaaacc tgtaatagat gcggcaaatc ctgtttttga accatttatt
      241 tgtaatgcga taaaattggc tattgggtaa taaaaaccat aggttggata atttttcaat
      301 ctttgataat tgatttccga ttatgaaaaa taaaactgag aaggagaaaa taattgagta
      361 actaaaagta ctcaggccat aagtatgcat gaaaattgat ggtgatattg atatgtagca
      421 aaatagagaa ctgagtatta atgagacaag tatagtgccg cagataaact ctttggtcac
      481 aaaggatgca ctgttttgac atattacttt ctcatttgca tgagttatct ttggtgataa
      541 taacattatt ataatactga acagcatcat aacaagaaat ataacattcc agttataata
      601 ttcaataatc aatgcgcgag gtgctactaa tggtattact gcgcttacag atgatatatc
      661 agaaagcgcc cgttttagtt ttaatggagg gtgatcttac ccaccaatag tggacacagg
      721 actaagtgag taaactctca accagaggtg actcatgaca aaaccagtat caatcagcaa
      781 gaagccccgt aaacaacata cgcctgaatt tcgtaacgaa gccctgaaac tcgctgaacg
      841 catcggtgtg gccgccgcag cccgtgaact cagcctgtat gaatctcagc tttatgcctg
      901 gcgcagtaaa cagcagcaac aaatgagttc gtcagagcgc gaaagcgaac tggccgctga
      961 aaatgtccgc cttaaacgac aactggcgga gcaggctgag gaactgtcca tcctccaaaa
     1021 ggccgcgaca tacttcgcga agcgcctgaa atgaagtatg tctttatcga aaatcatcgg
     1081 gcagagttca gcatcaaagc gatgtgtcgt gtacttcggg ttgcccgcag cggctggtat
     1141 gtctggctca ggcgtcgtca ccagatgagc ctgcgccaac agtttcggct cacctgcgat
     1201 gccgctgtcc ataaggcatt ctttgaggca aagcagcgat acggtgctcc ccgccttgct
     1261 gacgaactgc cggagttcaa tattaaaacc attgccgcca gcctgcgtcg tcaggggctg
     1321 cgggcgaaag ccagccggaa gttcagcccg gtcagctacc gtgcacatgg cctgcccgta
     1381 ttggagaatc tgctggagca ggacttcagc gccagtggcc cgaaaccaga agtgggcggg
     1441 tgacatcacg tacttgcgta ccgatgaggg ctggttgtat ctcgcagtag tcatcgacct
     1501 gtggtcacgc gccgtcattg gctggtcgat gtcaccgcga atgacagcac aactggcctg
     1561 tgatgcactg caaatggcgt tgtggcggag aagacgcccg gaaagcgtca ttgttcatac
     1621 ggaccgtggt ggtcaatact gttcagggga ttatcaggca atgctgaagc gacacaacct
     1681 gcgtggcagt atgagtgcga aaggctactg ttatgacaat gcctgtgtgg aaagcttctt
     1741 tcattcgctg aaggtggaat gtatccacgg ggaacgcttt agcagccggg aaataatgcg
     1801 ggcaacggtg tttaattata tcgagtgtga ttacaatcgc tggcgtcgtc acagtgcctg
     1861 tggcggtctc agcccggaac aatttgaaaa ccataatctc gcttagggcc gtgtccacat
     1921 tacgtgggta ggatcagtat gggtcgtctt gtatttgtca tactgcccaa tctcaccaat
     1981 cgaaaatgct ccttttggca aaggttcaag caggtctctg ggaatggagg tcgctggcgt
     2041 cggatataaa aaaccctcac ggcttagcaa ggctggcgtc actatcggtg taacaatatc
     2101 aggcgtgcct gcggaacggt ccttaccatg ctgcagtaac cggaaataaa cttttgggcg
     2161 taagatgaca ttgacagttt cgacatcgtc tttttctccc ttaaaaaatt tattgacaat
     2221 cgcttcgtca agacgtcccg ccgctatccc atgccagtga gtgaaatttt gggcgtcttt
     2281 ccgttcaaaa cagccctttc ctgactcagc atctgcaagc gagttgcgcc agtatgaggc
     2341 aaaccttaaa gcattttcat ccattaccag tcctgtgact caatgaaatc cattgattta
     2401 tcgaatataa aaccaccgta tggacaacta ttgtccatat taaaaaagtt ggatgcaatg
     2461 ttttgtttat aattcgatat caaatgtact ggttgtacga ggttcagaac acaagcacat
     2521 tcaacagtac gtcaggacta ttgatttcat aacagcgaat acaatttttt actgtttata
     2581 atggctgcct gtgttggacg gggataccag ttcaaccgaa aacgccagag gaggggatta
     2641 cccgctggca gggtaaatct gtggtttttg atgccagcta taacgtgttt gacgataaag
     2701 tattacgaaa gaactttaat gagttcatgg agtaattcgt taaagtaatt tacaacgcta
     2761 tccttttgca aaggaaactt tcagaaaata accccagggt aatcacctgg ggaaggaaca
     2821 cagtgttatt attggataat aaattattac ttgttttaaa gttacactaa tgcaatatct
     2881 actgatactg gatgcttgaa tgtttcatcc ctgctgcgga aaagggcaat atccagggcc
     2941 agatatattc accgttcata tttctgcatt tctgtacaat ttcctcagaa agtgtcttgt
     3001 caattctttt ccacaggtgc tcctgctgtc ttaaaatact ttcgcatatt tcgaaatcat
     3061 cttcctgaat cagcatttcg gcgcatttaa tgatttctga tcgttcatgc agcacggagg
     3121 aatggttata gagccagttt acaaacagca atgcttccgg atattgtccc cataatggtg
     3181 agtatgttgc atagtaatct tctgccgtat ccagagagcc ggagaagtca tgccacacag
     3241 caagtggaaa tggatgcaga tttccgcaca gcatttctgc gatgtaagtg tttgtggcaa
     3301 agccatggcg aaaagctgtg gcggccttaa catgttcccc gttcagaaaa tgcaggagac
     3361 cgagttcata ccagtaaggt gaaaattcat cagcatgctc tttcagtaca ctgaaggctc
     3421 gctcatgatc gccggtgcgt aacagctctg aacccaacaa ccagcgagcc ccctgattat
     3481 cttcaggatt gtatgccagt attttatcag tgagcataac cgcatcctga tggcgctgta
     3541 agtgcacatt tgcgagaatt gctgcataaa gcgcgcgcag ataaggccgg ttttcgggat
     3601 gcatccagat tatttcacca cagaaacttt ccgggatgat acgattacct gccgccagtc
     3661 ctttaagagc ggcgttcagt gccttacgcg gggcattctg ttccagaaat gcatatgcca
     3721 gatgagcgtg tatatcaata aagtccggct cccgttgagc aaggcgtcgg agtttgttga
     3781 tgtaactcgt ctcgctgatt tcatcagcgt cgtatgcatc aagcacttct tcaaactccc
     3841 ccagcaatgg gccaaaccag gtatccggaa atacgaaata acccttgtta tcgtgcactt
     3901 caaagcataa tctatcgttc attttctgag cctgtaaaac atgtggtgat atagtctgag
     3961 agcataaaag ttgagattct gtgtatcaag tcctgtcttg gcaccgaatg aatattgctt
     4021 acgaagcccc ttcatgcttt ccttgagaac aataatttta tctttatgtg aatctcattt
     4081 tttataatga tgtcagatga ttgaaatgct aaatt
//
LOCUS       DAEBXN010000226         4114 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.228, whole genome shotgun sequence.
ACCESSION   DAEBXN010000226 DAEBXN010000000
VERSION     DAEBXN010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4114
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.228"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(<1..158)
                     /locus_tag="KJL99_004265"
     CDS             complement(<1..158)
                     /locus_tag="KJL99_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HBD4438760.1"
                     /translation="MDQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYL"
     gene            complement(308..469)
                     /locus_tag="KJL99_004266"
     CDS             complement(308..469)
                     /locus_tag="KJL99_004266"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438761.1"
                     /translation="MILRHRTGPAFQAIKDSLAVGLNALTLTDITKNATYGVEIESLV
                     LEINAPASS"
     gene            complement(466..654)
                     /locus_tag="KJL99_004267"
     CDS             complement(466..654)
                     /locus_tag="KJL99_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HBD4438762.1"
                     /translation="MAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPE"
     gene            914..1249
                     /gene="iraM"
                     /locus_tag="KJL99_004268"
     CDS             914..1249
                     /gene="iraM"
                     /locus_tag="KJL99_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HBD4438763.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1530..1638)
                     /locus_tag="KJL99_004269"
                     /pseudo
     CDS             complement(1530..1638)
                     /locus_tag="KJL99_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001416037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
     gene            complement(2161..2237)
                     /locus_tag="KJL99_004270"
     tRNA            complement(2161..2237)
                     /locus_tag="KJL99_004270"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2201..2203),aa:Arg,seq:tct)
     gene            complement(2339..2414)
                     /locus_tag="KJL99_004271"
     tRNA            complement(2339..2414)
                     /locus_tag="KJL99_004271"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2379..2381),aa:Ile,seq:cat)
     gene            complement(2559..3380)
                     /locus_tag="KJL99_004272"
     CDS             complement(2559..3380)
                     /locus_tag="KJL99_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HBD4438764.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKIQYPDGKPPVWAKCTKGVYPFYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(3395..3751)
                     /locus_tag="KJL99_004273"
     CDS             complement(3395..3751)
                     /locus_tag="KJL99_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBD4438765.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMH"
     gene            complement(3764..>4114)
                     /locus_tag="KJL99_004274"
     CDS             complement(3764..>4114)
                     /locus_tag="KJL99_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HBD4438766.1"
                     /translation="PQQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYKRWVKTQPCA
                     CCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLI
                     FRFLDHAFATGVLG"
BASE COUNT         1155 a         1118 c          793 g         1048 t
ORIGIN      
        1 agatatgtca gcagcccaaa cagcagactc cccagcacgc ctattgccgc ccactgagac
       61 ggggaaaccc tgtccagcaa ctgcaggaac cagtagcccg ttcccaccgc tgacgtggtg
      121 tatgacacac ctgttgtgat tttttccatc tggtccatac cccgtctccc gttatccgga
      181 agctgacaac aataaaaaag ccaccagtta actactgatg gctctgataa ctcatgcaag
      241 cgtctcagac gacccactga cactaccggt gagtttaacg ataccttcca tttgactggc
      301 tcacttttta tgatgatgcc ggtgcattta tctccagcac cagactttct atctcaacgc
      361 catacgttgc atttttggta atatccgtca gcgtcagtgc atttagtccc actgccagac
      421 tgtcttttat ggcctggaat gccgggccag tacgatgacg tagtatcact ccggctcagt
      481 tgcaccactg accaccacat caccttctgc tgcaatcgcc tgcatcaggg tataaggggt
      541 tatggccacc ggactaccaa acggctgcca gccctctttc agtttatgtg tcagcttttc
      601 cgcaagatct gacggcggcg ccgccctgac aacatcatag tgtttaaatg ccatggttct
      661 ttccaacatc tgaaaaataa ttctttaaaa tacctgacat gtaatacaga aaaaacacaa
      721 aaccatacct taaataaaaa cctgattatc aagcagatat gcatggataa actacaagac
      781 gagatataaa ccaccctgca tttaaataaa caataaacaa catcagaaaa ataattctgt
      841 tctatggttt aattcaaaaa tatcatttat acttttcaga acatcaccag caaggcataa
      901 acaaggaaac taaatgaagt ggattgtgat tgatacagtt atccagccat catgcggaat
      961 atctttttca gtcatatgga gtaaaataaa attaataatc tggtatcaat cggatgcttt
     1021 cttacctcct gaaagtatat ttacactgac tcacacaggc atcatgctca ataacaaagt
     1081 gctacctgta accatttaca acgtagtacc attcaataaa acattctgga atttaatcaa
     1141 aaacagccag gaatgcccta caaatacaga taacgtattg aatgaatgct ttaataaccg
     1201 ttgcactctg caaatatgcc cttatggact aaaacaacaa agtccataag gagtttactc
     1261 acatctgaca aaatcaatat aaacagcccc tccggagagg ggctggagag tggcgctatg
     1321 tgccattcca tggtgccggg tgcctcccgg tgaattcagt accagcacct gaatccgcga
     1381 ttatcccata tacctactcg ctgattgccc ctccgcacag ggggattcac catgccagtt
     1441 tcttttaaca aactccccgc aaaccagaca acagtcaacc gcctgaattg tgaagtattt
     1501 aaaaatttct cccgctaact gatacccggc taacagtctg gcgttttctt tttcagcaac
     1561 gggaaagcaa caaccaccac acccgccacc agcacaccgt cagccggcac tgacattatc
     1621 cggctgctgc aatgccatta acaaaaacag taagcaatca ctttttaccg taacaggtga
     1681 taatccagat atgtatctac cccagatgag taatccgaag ttcatccata ccacaggtcc
     1741 tggctattct gttgtactcc tgaacaagag caaataattc tgaattagca accatgaact
     1801 catcgcaaac cctctgtata gcatcactat tcagaataat aacgtctctt cccgaaagac
     1861 gatcaggagt acagaacaaa actgtcaaac ggctgaaggc ctttgctcgt gctgcattga
     1921 ctatatcaat acgctgccta aggatgaaac accccgacgc ctcatcaata ttcactctac
     1981 ccacaccata tgaatgataa atacttaatg ctgaaaaaac cattagaccg tataacaaat
     2041 actcaatcaa tacttaacag aacttttatt tttgacaaat atataatatt ttcaacaata
     2101 tcctgagcca ggtatatttc agtataaggc tctgccggaa ggaatctgga agaatgaata
     2161 tggcgcgctg tactggattc gaaccagtga ccgattgctt agaaggcaat tgctctgtcc
     2221 ggctgagcta acaacgctga ataccgataa tggaccgcca tcggggaccc gccccccgca
     2281 ccaacaaccc tgttatcgtg tcgtctgctc ttcctgataa gctaatggcg gtttgtgatg
     2341 gtggcccttg ctggatttga accagcgacc tggcgattat gagtcgctcg ctctcaccac
     2401 tgagctaaag ggccgggagc agaataataa cggtccgtaa ttaatttcgc aataaaaaaa
     2461 cccgctcagc ggcgggttgt agaaactctt ctaacgccag gcataaaacg cccatcatta
     2521 tgacgaattt accacagatt ccggaaaaat caaccttgtt acctagttac cttttttaac
     2581 tgccgctcag cccatgcttc ttcaatatca aaccgggtca ccagcgcatc ataaaatttc
     2641 ttaactgttt tttcccatga cgcgcgtgtt atctggtttg tcacctcgca tatagcatta
     2701 aatgcctccg ttgatggtag tctttcatag ccacgaccac cacaacgctg gcagtctctg
     2761 ataacaggca taccacgttt taccgactct tcacgatgaa tggcgacacc acgcccacgg
     2821 caatccttac aggcggtgga aacctcaccc tttccgccac actccggaca ggcaactttt
     2881 accacctccc tgactttttt ccattcttcc cagtaaaacg gatacacacc tttcgtacac
     2941 tttgcccata ccggcggctt accatccgga tactggatct tgtttgtaaa aactatgctt
     3001 tcaataaatt tttccccata gcaacaaggg cactgctttt tactcgctgc gctgcgggca
     3061 taatcctcaa aagcgtacga agccataatg cgcatcacta ccggttttat ttctgccgga
     3121 agttttctca acgccgccac acgatcgcac cgactgagtg cataatctgc cagtaattct
     3181 gttgcccgcg ccctgtcatt catactgatg cccattttcc ccaggaacgc agaaaaaccc
     3241 atctcagccc gattctgtgt catgccctgc gcggccatca catcagtgat actcagcgca
     3301 tcttttgacg ttgaggccga tgcatcggtc aggccagggg attttgggga gtagtatttc
     3361 ggtaaatctt ccagtttcat tttttgacct gctcttaatg cattatgggg taaatcttca
     3421 cccccagacg tccaccagat actggctgac cacgaacgat attgatttca tcaaactgct
     3481 catcgtccat taacactccc gcatgcgtca gcgcatccag cggtgctttc aggatattgt
     3541 ccaggtcgcg acgacgctta tccggtggct ctgcaatcac ctttatcgcc agccttccgg
     3601 acaggcttaa tttcagccgc tgctggcgaa caataagcgc cacagcccgg cgataacgct
     3661 ttccctcctc cgagataaaa tatgtgctgc cacggcgtcg ccagtaagtg ttcaccgtcg
     3721 gcgggtaagg taaaaccaaa tctatgagca tcagtcacct cttttaccca agcacgccag
     3781 ttgcaaaggc gtgatcaaga aaacgaaaaa ttaaatcaac ctgagaacca tgcttttctt
     3841 cgaacgccag aggatccgca tgaagctcgt tgtgatgctc ccgacacagc ggtagcgtga
     3901 aaatatcgtg agatttggtc cccattccgc cctgaccatg accaatcagg tgatggggat
     3961 cgtcggctgg cttaccacaa cacgcacacg gctgtgtctt cacccagcgt ttgtatttct
     4021 cgttaaccca gcggcgacgt ttaggtcgtt tcatgaaaga ttccggagac tcaggatcaa
     4081 cggcaatgct gaccaccgtc ttttcctgtt gtgg
//
LOCUS       DAEBXN010000227         4091 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.229, whole genome shotgun sequence.
ACCESSION   DAEBXN010000227 DAEBXN010000000
VERSION     DAEBXN010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4091
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.229"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(46..747)
                     /locus_tag="KJL99_004275"
     CDS             complement(46..747)
                     /locus_tag="KJL99_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBD4438767.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCIGRLKSGSDAESTE
                     LQKTMTVTAEEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQYRA
                     QYAKGEGKAQITFSDAVMPYLTQLKGQFTRVVIKNISNLSRSYSIRIYELLQQFRSTG
                     ERIIALDDFRSMLDIEHKYQTYKSLNQQLLRPCVDELNQKSDLAVTLETIKKGRTVVA
                     LHFRFKEDKQIKMTI"
     gene            complement(1280..1570)
                     /locus_tag="KJL99_004276"
     CDS             complement(1280..1570)
                     /locus_tag="KJL99_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HBD4438768.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRK
                     PGETQLRAWLLHLLKTEVTRQADVSRIQPLIDELNALPKPVPKEVSENDQQA"
     gene            1741..3888
                     /locus_tag="KJL99_004277"
     CDS             1741..3888
                     /locus_tag="KJL99_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HBD4438769.1"
                     /translation="MAIYHLSMKILSRSKGYSAVASAAYRAGEKILDERTGVIHDYTR
                     KNGVASAVILTPANAPAWCANRAELWNAVEKAERRKNSQLAREFELAIPRELAQDAAR
                     ETVLNFVRENFVSRGMIADVAFHNLGGSNPHAHIMLSMRAITPDGFVEKVREWNDWTH
                     AETWRGSWADHANRALANAGYQEEIDHRSYERQGVEKTPGIHLGKSACAMEKWGIETE
                     RGEQNRLINRLNLEIQVSRDELRNSELTEPARRMADLLNIELPDNATSYILHDIIEAL
                     PTDSNAAWKLTSKAMSMMADMQATRRRWEDLNTERKEAMSHAASLKKSSPFSSGFSRI
                     PLMQWVAPEYGREQEKIQSLSQRMEKLRQHHWKVEKQDIPASQKAFETQWQQWGASGL
                     ADLREQLRKREEELRRKEQEETERRRAEQLRRNDNAVMDSGVLKAVVTGYGEAPMPGN
                     GEMTCYLLLHNRNGEYTLWGNELEKYRARIFESVDLMRDRSGYICERSEIGQRPPLQH
                     VYSSATFEQLLTQVCQTWPQHTRDLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQG
                     RLPPTLMPVMSSVDRETRQLQLLLVMGVDDSMGGVVRLNGTLYPAFAVPTADNSQLVI
                     NALTDKGLRFAGYGEAVNHDADSPARPAPELMQFHLKTWSEPLFAIVNTPEKQPDHLF
                     RSLGFERTWDEWQRDEYARTHATERRHDRGWSQ"
BASE COUNT         1082 a         1026 c         1000 g          983 t
ORIGIN      
        1 aaaaaaaacg cgtcagagag cgttttagag gccttaaaat cgattctaga tcgtcatctt
       61 gatctgtttg tcttccttga agcgaaaatg cagggctaca accgtgcgcc ccttcttgat
      121 cgtctcaagc gttacagcca agtcgctttt ttgattcagt tcgtcaacgc aaggtcttaa
      181 taattgctgg ttcagagact tatatgtttg atatttatgc tctatatcaa gcattgagcg
      241 aaaatcatcg agggcgataa tacgttcccc tgtgctgcgg aattgctgga gaagttcgta
      301 tattcttatg ctgtaacttc ttgataaatt ggagatgttc ttaattacaa ctctagtaaa
      361 ttgccccttc aactgtgtca gataaggcat gaccgcatct gaaaaagtga tttgggcttt
      421 gccctcacct ttagcgtact gtgcccgata ctggatccag cggaactcct cgcgcttctc
      481 atcgtcctta aggatgatcg acctgtccca caaacgatca attgcttcct gtagctgaac
      541 ctcggcattt ttccgcccca tatcagggaa tgagtcgaaa tactcttcgg cggtaacagt
      601 catggtcttt tgcaattcag tggattctgc atcagaaccc gacttcaatc gaccaatgca
      661 aagtaacaag aatcgctgtt cctgaagagt caatttatag ctggcctcaa ccagtgcatt
      721 ggatttgtag gcaatcagat ccgacattgt aaatccctca acctaaggta atgagtaaaa
      781 tataaacatt cctttaggta aaggcaatat gatttaattt taatatccct tttcctgtga
      841 atattacctt aaatcatatt gcctttacct taaatcatat tgcctatcca ccttaaatca
      901 tattgccttt accttaaatt atattaccta tagtcttcta aactcttgaa tttcataaga
      961 aaattcgacc ctaaaacaag tattaaaaac aagatcttaa aaacaagatc taaaaactta
     1021 acggcgagcc tgtggaaaac ttggggtgtt caaaacaaaa aatcatgaaa aacaacaggt
     1081 tacaaaaaag caggttcgca agctttagca aaaccccgct caaccctgtg gaaatccagt
     1141 attcatgcgg gttcagcgca tatcacctgt cgaaaaaccg tgcgtatgtg ctcaatctgg
     1201 cgacggattc acttccgctt cctcgcccac tcgctcgcta acgctcggtc gctggggctg
     1261 cggcgagcgg acggtgcatt cacgcctgtt ggtcgttttc tgacacttct ttggggactg
     1321 gttttggtaa cgcgttcagt tcgtcaatca acggctgtat cctgctcaca tcagcctgcc
     1381 gtgttacctc tgtcttcagg agatgaagca accaggctcg tagttgtgtt tccccaggct
     1441 tcctgacctt tgccagcaat gccgctccca caatgacctt ctgcgctgtg tccaggctcc
     1501 tgctcttcgc cttcagacgc tgcaatctgg cctcagcatc ggcaatcttc tgttcgagtg
     1561 ttctactcat tccgtaactc cgtgcgcggt gaaaaatcgt attttagcgc gtcactggta
     1621 gtttaaaaac tgaactggca taatgcacag caaatcacga agtgcgcact tatacaaact
     1681 ctccttcgtc gagtttgtgt gcgccctgcg gggctaaaag aaaacggcaa caacgacacg
     1741 atggcaattt atcatctcag catgaagatt ctttccagaa gcaagggcta cagcgccgtt
     1801 gcttctgctg cctatcgtgc cggagaaaaa atactggatg agcgcaccgg agtgattcac
     1861 gactacaccc gcaaaaacgg tgtagccagc gcagtgattc tgacgccagc aaacgcccca
     1921 gcatggtgtg cgaaccgcgc cgaactctgg aatgctgttg aaaaagccga gcggcggaaa
     1981 aactcacaac ttgcgcggga gttcgaactc gccatccccc gtgaactggc gcaggatgcc
     2041 gcacgggaaa cagttctcaa cttcgtaagg gaaaactttg tcagtcgcgg catgatcgcc
     2101 gacgtggcct ttcacaatct gggcgggtca aacccacacg cgcacatcat gctatccatg
     2161 agggcaatca cgcctgacgg cttcgtggag aaagtccgtg agtggaatga ctggactcac
     2221 gccgaaacat ggcggggatc atgggcagac catgcgaaca gggcgctggc gaacgccggt
     2281 tatcaggaag agatagacca tcgttcatac gagcgacagg gggttgagaa aacgcctggc
     2341 attcacctcg gaaagtcggc ctgtgcgatg gaaaagtggg gcatcgaaac agaacgcggc
     2401 gaacagaatc gcctgattaa caggcttaac ctggaaatac aggtttcccg cgacgaactc
     2461 aggaacagtg agctgacaga gcccgcacgc cgtatggctg accttctgaa tattgagctt
     2521 cctgacaacg caacttcgta catactgcat gacatcatcg aagcgttgcc gacggacagt
     2581 aacgcagcat ggaaactcac cagtaaagcc atgtcgatga tggcagatat gcaggcgacg
     2641 cgcagacgct gggaagatct gaacaccgaa cgaaaggaag cgatgagcca tgctgccagc
     2701 ctgaaaaaat catcgccgtt ttcgtccggt ttttcccgta ttcccctgat gcaatgggtt
     2761 gcaccggaat acggccgcga acaggaaaaa atccagtcgt tgagccagcg catggaaaaa
     2821 ttgcggcagc atcactggaa ggttgagaaa caggacattc ccgcgagtca aaaagcattt
     2881 gaaacacagt ggcagcagtg gggtgcatct ggccttgcag atctccgaga acagttgcgt
     2941 aaacgtgaag aggaattgcg ccggaaggag caggaggaaa cagaacgtcg tcgggcggag
     3001 cagctcagga gaaatgacaa tgccgttatg gattcagggg tactgaaagc agtcgttacg
     3061 gggtatggcg aggcccccat gccaggtaac ggggaaatga cctgttacct tctgcttcac
     3121 aaccggaacg gagaatatac gctctggggg aacgaactgg agaaataccg cgccagaatt
     3181 tttgagagtg ttgatttaat gcgtgacagg agtggttata tctgtgagcg aagcgaaatc
     3241 ggtcaacgtc caccactgca acatgtttat tcctctgcga cctttgagca actgctgacg
     3301 caggtttgcc agacatggcc gcaacatacc cgcgatctcc gccagccgaa gacatggccg
     3361 gagtctttct gtcttggaga agaccgccag ccagccatgc catcactggc ggcaaggaag
     3421 gtggatttta cgcagggacg tctgccaccg actctgatgc cggtaatgag ttctgtcgac
     3481 cgtgaaaccc gccagcttca gcttttgctg gtcatgggtg tggatgactc tatgggcggt
     3541 gttgtccggc ttaatggtac gctgtatccc gcgtttgccg tccccacagc cgacaacagc
     3601 cagctggtaa tcaatgcact gaccgacaaa ggtttacgct ttgcaggcta tggtgaagcg
     3661 gtaaaccacg atgctgacag tccggcacgt cctgcgccgg aacttatgca gtttcatctg
     3721 aaaacctgga gcgaaccgct ttttgctatc gtcaacacgc cagaaaaaca gccggatcat
     3781 ctgttccgct cgctgggctt tgagcgcacc tgggatgaat ggcaacgtga tgaatatgcc
     3841 cgaacacacg ccaccgagag gagacatgat cgcggttggt cacagtgaat gtgaccaata
     3901 ttttgttttg tttaacatcg tgttatgttc gtcgatgttc atcgacaggg atgaatgcaa
     3961 gaagcacaca cataacacaa atcaacttta tagttttccc gcccccgaaa aattgggggt
     4021 tcttttttca ctgtgccaga gcgtttatga cgcaattttt tacttcacca gtaccttcgc
     4081 atgccaaaca c
//
LOCUS       DAEBXN010000228         4087 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.230, whole genome shotgun sequence.
ACCESSION   DAEBXN010000228 DAEBXN010000000
VERSION     DAEBXN010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.230"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(46..753)
                     /locus_tag="KJL99_004278"
     CDS             complement(46..753)
                     /locus_tag="KJL99_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBD4438770.1"
                     /translation="MTSNPLIAYKSNALVEASYKLTLQEQRFLLLCISRLNSGTDVAS
                     PELQKTMTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQY
                     RAQYARGEGKAQITFSDAVMPYLTQLKGQFTRVVIKNISNLSRSYSIRIYEILQQFRS
                     TGERIIALDDFKSSLMLDGKYKDFKTLNRDLIKPCVDELNKKSDLAVTVETIKKGRTV
                     VALHFRFKEDKQIKMTI"
     gene            complement(1285..1539)
                     /locus_tag="KJL99_004279"
     CDS             complement(1285..1539)
                     /locus_tag="KJL99_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HBD4438771.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRK
                     PEEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
     gene            1718..3877
                     /locus_tag="KJL99_004280"
     CDS             1718..3877
                     /locus_tag="KJL99_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HBD4438772.1"
                     /translation="MAIYHLSMKIISRNSGYSAVASAAYRSGSLMLDERTGLTHDYTR
                     KSGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPRELPQDAAR
                     ETVLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTH
                     AETWRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETE
                     RGEQNRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEAL
                     PKDSNAAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRI
                     PLMQWAAPEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGL
                     AALRVQLLKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAP
                     MPGNGEMTCYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHP
                     PLQRVYSSATFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVD
                     FTQGRLLPTLMPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNS
                     QLVISALTDKGLRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQP
                     DHLFRSLGFNRTWDEWRREEDARTHATERRHDRGWSQ"
BASE COUNT         1065 a         1045 c          993 g          984 t
ORIGIN      
        1 aaaaaaaacg cagcagggag cgttttaggt gccttaaaat cgattctaga tcgtcatctt
       61 gatctgtttg tcttccttga agcgaaaatg cagggctaca accgtgcgcc cctttttgat
      121 cgtctcaacc gttacagcca ggtcgctttt tttgttcagt tcgtcaacac agggttttat
      181 tagatccctg tttaacgttt tgaaatcttt atatttacca tcaagcatca gtgatgattt
      241 aaaatcatcg agggcgataa tacgttcccc agtactgcgg aattgctgga gaatttcgta
      301 tattcttatg ctgtaacttc ttgataaatt agaaatattt ttaatgacaa ctctggtgaa
      361 ttgccccttc aactgtgtca gataaggcat taccgcgtca gaaaaagtga tttgtgcttt
      421 cccctctccc ctcgcgtact gtgcccgata ctgaatccag cggaactcct cgcgcttctc
      481 gtcgtccttg agaatgatcg acctgtccca caaacgatcg atcgcttcct gtagctgaac
      541 ctcggcattt ttccgcccca tatcagggaa tgaatcaaaa tattccgcag ctgtaatggt
      601 cattgtcttt tgcaattcag gtgaagctac gtcagtaccg gagttaagtc ggcttatgca
      661 gagcaataag aaacgctgtt cttgtaaagt tagcttgtaa ctggcctcaa ccagcgcatt
      721 ggacttatag gctatcagag ggtttgatgt cattttaacc accaggccaa aaatgaggtg
      781 aaataaaaac ctatcacctt tagcattggt gagcaattat attttcaccc cccattttcc
      841 tgtgaatatt accttaaatc tcctcacctt taccttaaat tacctcaccc aagcacctta
      901 aattatatca cctttacctt aaatcatatc acctatagcg ttgtaacatt ttgttttcta
      961 taaggaattt tggcgctaaa acaagtatta aaaacaagat cttaaaaaca agatctaaaa
     1021 acttaacgac gagcctgtgg ataacttgga gtgctgaaaa caaaaaacca ataaaaacaa
     1081 tgcgttgcaa aaaagcagaa ttgcaagttc cgaatacacc ccctccaacc ctgtggaaat
     1141 ccagtattca tgcgggttca gcgcatatca cctcccgaaa aaccgtgcgt atgtgctcaa
     1201 tcaggcgacg gattcacttc cgcttcctcg cccactcgct cgctaacgct cggtcgctgg
     1261 gactgcggcg agcggacgat acaatcactg tcctggcagc gcttccagct cgttaatcag
     1321 cggcagtatg cgcgtcacat cagcctgtcg tgtcacctct gccttcagga actggagtaa
     1381 ccaggcacgc aactgcacct cctccggctt tctgaccttt gccagcaatg ctgcacccac
     1441 aatgaccttc tgcgctgtgt ccagactcct gctcttcgcc ttcaggcgct gtaatctggc
     1501 ctcagcatca gcaatcttct gttcgagtgt tctgctcatg atgtaactcc gtgcgcgatg
     1561 aaaaatcgca ttttagcgcg tcactggtag tttaaaaact gagctggcat aatgcacgga
     1621 aaatcacgaa gtgcgcactt atacaatctc cactccgttt cgattgtgtg cgccctgcgg
     1681 ggctgaaaga aaaccacaaa aaggcattac ggcagaaatg gcgatttatc atctcagcat
     1741 gaaaatcatc tccagaaaca gcggctacag cgccgttgct tctgctgcct atcgttccgg
     1801 ttcactgatg cttgatgagc gtaccggact gactcacgac tacacccgca aaagcggtgt
     1861 ggctgaagct gtgattctga ctcccgcgac agcccctgcc tggtgtacga accgtgccga
     1921 actctggaat gccgttgaga aggccgaacg ccggaagaac tcacaacttg cgcgggaaat
     1981 tgaactcgcc atcccccgcg aactaccgca ggatgccgca cgggaaaccg ttctcgcctt
     2041 tgtcagagaa aactttgtca gtcagggcat gattgctgat gtggcgttcc atcacatgga
     2101 taaaacaaat ccccatgcgc acatcatgct gaccacgaga gctgtcggac ctgctggatt
     2161 cggtggaaag gtcagggact ggaacgaccg gacacatgcc gagacgtggc gtgcatcatg
     2221 ggctgaccat gcgaacaggg ctctggcgaa cgccggttat caggaagaga tagaccatcg
     2281 ttcatacgag cgacaggggc ttgagaaaac gcctggtatt cacctcggaa agtctgcctg
     2341 tgcgatggaa acacgcggta tcgaaacaga acgcggcgaa cagaatcgtc tgattaacag
     2401 acttaacctg gaaatacaga tttcccgtac cgaactcaga aacagcgggc tgacagagcc
     2461 tgcccgccgt gtggctgacc ttctgaatat cgtgcttcct gacaacgcaa cttcgtacac
     2521 actacgtgac atcatcgaag cgttgccgaa ggacagtaat gcggcatgga aactcaccag
     2581 taatgccatg tcgatgatgg cagatatgca ggcgacgcgc agacgctggg aagagctgaa
     2641 caccgaacga aaggaagcga tgagccatgc tgccagcctg aaaaaatcat cgccgttttc
     2701 gtccggtttt tcccgtattc ccctgatgca atgggcggca ccggaatacc gtagcgaaca
     2761 gggaaaaatc cagtcgctga gccagcaaat ggaaaaattg cgacagcatt accggaaggt
     2821 ggagaaacag gacattcccg cgagtcagac ggcatttgaa aaacagtggc agcagtgggg
     2881 ggcatccggc cttgcagctc tcagagtcca gttgcttaaa cgtgaagagg aattgcgccg
     2941 gaaggagcag gaggatacag aacgtagtcg cgcggagcga cgagcggaac agctcaggaa
     3001 aaatgacaat gccgttatgg attcaggggt actgagcgca gtcgttacgg ggtatggcga
     3061 ggcccccatg cctggtaacg gggaaatgac ctgttacctt ctgcttcaca accggaacgg
     3121 agaatacacg ctctggggga acgagctgga gaaataccgc accagaatct ttgagcgtgt
     3181 tgatttaatg cgtgacagga gtggttatat ctgtgaccga agcgaaatcg gtcaacatcc
     3241 accactgcaa cgtgtttatt cctctgcgac ctttgagcaa cttctgacgc aggtttgcca
     3301 gacatggccg caatataccc gcaatctccg ccagccgaag acatggccgg agtctttctg
     3361 tcttggagaa gaccgccagc cagccatgcc atcactggca gcaaggaagg tggattttac
     3421 gcagggacgt ctgctaccga cgctgatgcc ggtaatgagt tctgtcgacc gggaaacccg
     3481 ccagcttcag cttttgctgg tcatgggcgt ggatgactct ctgggcggtg ttgtccggct
     3541 taatggtacg ctgtatcccg cgtttgccgt tccctcagcc gataacagcc agctggtcat
     3601 cagtgcactg accgataagg ggttacggta tgcagggtat ggtgttgccg taaatcacga
     3661 tgcagacagc cacatatcac ctgcgccgga acttatggag tttcatctga aaacccgtga
     3721 ggcaccgctt tttgctgccg tcaacacacc agaaaaacag ccggatcatc tgttccgctc
     3781 gctgggtttt aatcgtacct gggacgaatg gcggcgcgag gaagatgcac gaacacacgc
     3841 caccgagagg agacatgatc gcggttggtc acagtgaatg tgaacaatat tttgttttgt
     3901 ttaacatcgt gttatgttcg tcgatgttct tcgatgttca tcgacaggga tgaatgcaag
     3961 aagcacacac ataatgcaag catagttttg tttttccgcc cccgaaaaat gggggttctt
     4021 ttttcgctgc attagagcgc ttttgacgca atttttcact tcaatagtac cttcgcacta
     4081 ctaacac
//
LOCUS       DAEBXN010000229         4065 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.231, whole genome shotgun sequence.
ACCESSION   DAEBXN010000229 DAEBXN010000000
VERSION     DAEBXN010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4065
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.231"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(139..3654)
                     /locus_tag="KJL99_004281"
     CDS             complement(139..3654)
                     /locus_tag="KJL99_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBD4438773.1"
                     /translation="MDENALGFASYWRNSLADAESGKGSFKRKDAQNFTHWHGIAAGR
                     LDEAIVSKFFEGEKDDVETVDVILRPKVYFRLLQHGKDRSAGAPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTTFSINFDDSVDKTAETD
                     EEREARYAALQQEWRQYLYDSERLLKSVAGDWIEKPEQYELAEHGYIVKTAQSGGASS
                     HILSLYDHLLVCNKDVPLFNRFASREVHAAESLLAPGAKFSDRLGHSGDKFPLAKAQR
                     DALSHFLDARHGDILAVNGPPGTGKTTLVLSIIATQWARAALEKSEPPVIIATSTNNQ
                     AVTNIIEAFGKDFSQGSGAMAGRWLPELKSFGAYFPSSSRKAEAAKKYQTEDFFNQVE
                     SKEYVEDALLFYLEKAKAAFPGKECSSPEKVIELLHGQLAAKSEQLIRLNATWQTLSQ
                     IRAARELIANDIEQYLDNLNKLLSGQEQKVTLLKSAKTEWKKYRAGESLIYSLFSWLP
                     AVRNKRQYQIQLFLEDKLGALIAGNQWSDPETIERNIDGLLNSAEREQTTYRQQIDSA
                     HEIVLKEQQAVQEWQRLAFDLGYEGDEELSFSQADELADTQIRFPAFLLTTHYWEGRW
                     LMDMASIDDLQDEKKKKGAKGVTARWQRRMKLTPCVVMTCYMLPGNMQISEHKGQRKF
                     EKSYLYDFADLLIVDEAGQVLPEVAAASFALAKKALVIGDTEQIPPIWSIAPAIDVGN
                     MLAEKILSGSTQEEITEKYTAIADLGKSAASGSVMKIAQFASRYQYDPELARGMYLYE
                     HRRCYDNIIGYCNTLCYHGKLLPKRGREESNLMPAMGYLHIDGKGELASSGSRYNLLE
                     AETIAVWLAENQQNIEAHYGKSLHEVVGIVTPFSAQVSTIKQVLGKQDISTGTNEKSL
                     TVGTVHSLQGAERAIVIFSPVYSKHEDGGFIDSDNSMLNVAVSRAKDSFLVFGDMDLF
                     EVQPASSPRGLLAKYLFESEKNALSFDYKERKDLKTAGTKIYTLHGVEQHDNFLNQTF
                     ENTSKHITIISPWLTWQRLEQTGFLDSMIAACSRGINVTIVTDRSYNTEHNDFEKRKE
                     KQQNFKAALEKLNALGIATKLVNRVHSKIVIGDDGLLCVGSFNWFSATREARYERYDT
                     SMVYCGDNLKGEIEAIYNSLERRQV"
BASE COUNT         1055 a         1027 c          844 g         1139 t
ORIGIN      
        1 gagcctctga ttcttccgga cagaagataa tcatgtcggt atgaatgtta taattaagga
       61 ctgtgcaata atagccattc cattcagcca tcttacgctc gaaaacgatc ggtaagctgg
      121 ctactcacca ttcctgctct aaacctgacg cctctcaaga ctattataaa tagcctcaat
      181 ctcacccttc aggttatcac cgcaataaac cattgatgta tcgtatcgtt catatcgcgc
      241 ttcccgtgtc gcactaaacc agttgaacga tcccacacac agcaaaccat catcaccaat
      301 aacaattttg ctatgaacac ggtttaccag ctttgtagca atacccagcg cattcagttt
      361 ctccagcgcc gctttaaagt tctgctgctt ctcttttcgc ttctcaaaat cattatgttc
      421 agtgttgtag cttctgtcag tgactatcgt gacgttaatt ccacgtgaac acgccgcaat
      481 catggaatca agaaaaccgg tttgctccag cctttgccag gtcagccatg gagaaattat
      541 cgtgatgtgt ttactggtat tttcaaatgt ctgattcagg aaattatcat gttgctccac
      601 accatgaagt gtgtagattt tggtcccggc ggtttttaaa tccttacgct ctttataatc
      661 aaaagagagc gcattcttct ctgactcaaa gaggtatttt gccagtaatc cccgtggcga
      721 tgaggctggc tggacctcaa acaggtccat atcgccgaag accagaaaac tgtccttcgc
      781 acgggagact gcaacattca gcatgctgtt atcgctatca ataaacccgc cgtcttcatg
      841 ttttgaatag actggcgaga atatcacaat cgctctttcc gctccctgaa gagagtgcac
      901 ggtgcccact gtgagcgact tttcattcgt gcctgtactg atatcttgtt tgcccagcac
      961 ctgtttgata gtggatacct gagcgctaaa aggcgtcaca ataccgacaa cttcatgaag
     1021 cgatttaccg taatgcgctt caatattttg ctggttctct gccaaccaga ccgctatcgt
     1081 ttcagcctca agcaaattat atcgacttcc actacttgcc agctctcctt taccatcaat
     1141 atggagatac cccattgcgg gcattaaatt gctctcttca cgccctcttt taggcaacaa
     1201 cttaccgtga tagcagagcg tattacagta tccaataata ttgtcgtagc accggcggtg
     1261 ttcatatagg tacataccac gagccagttc gggatcatat tgatagcgcg aagcaaactg
     1321 cgctattttc ataacgctgc cagatgcggc acttttacca aggtctgcga ttgccgtata
     1381 tttctcggta atctcttctt gcgtactgcc agacagaatt ttttccgcca gcatgttacc
     1441 gacatcaatc gcaggagcaa tactccatat tggcgggatc tgctccgtat cgccaatcac
     1501 taatgccttc ttagctaatg caaacgaggc agcagccact tcaggaagca cctgcccggc
     1561 ttcatcgaca atgagtaaat cggcaaaatc atacaaataa cttttctcga atttacgttg
     1621 tcctttgtgc tcacttatct gcatattacc gggcagcata tagcatgtca tcaccacaca
     1681 tggcgtgagt ttcattcgac gttgccaacg ggcggttacc cctttagcac cttttttctt
     1741 cttctcatcc tgcagatcat caatgctggc catatccatc agccaacgac cttcccagta
     1801 gtgagtcgtc agtaaaaatg cagggaagcg aatctgcgta tcagccagtt catcggcctg
     1861 tgagaagctc agttcctcgt cgccctcata ccctaaatca aatgccagcc tctgccactc
     1921 ctgaaccgcc tgctgttctt taagaacgat ttcatgggcg gagtcaatct gctgccggta
     1981 tgttgtttgc tcgcgctcag cggaattgag cagcccatca atattacgtt cgatagtttc
     2041 aggatcagac cactgatttc ctgcaatcag cgcgcctaat ttatcttcga gaaacagctg
     2101 tatttggtac tgtcgcttat tgcgaaccgc cgggagccag gaaaataatg aatagatcag
     2161 tgattcaccg gcgcgatatt ttttccattc cgttttagca ctcttcagta gagtgacttt
     2221 ttgttcttgt ccggaaagta atttatttaa attatcgaga tattgctcaa tatcattagc
     2281 aataagctca cgcgcagccc gaatctggct taacgtttgc catgttgcgt tcagtcttat
     2341 cagttgctca gattttgctg ccaactgacc atgcaagagt tcaatgacct tttcagggga
     2401 tgaacactct tttccaggaa aggctgcctt agccttttcc agataaaaca gcagtgcatc
     2461 ctctacatac tcttttgact caacctggtt gaagaaatct tcagtttgat attttttggc
     2521 tgcctcagct ttacgactgc ttgagggaaa ataagcaccg aagcttttca gctctggcaa
     2581 ccatcgcccg gccatcgcac ctgaaccttg cgaaaagtct ttcccgaatg cctcaataat
     2641 gttcgttaca gcctggttat tcgttgaagt cgcgataata accggaggct cagatttttc
     2701 gagagccgct ctggcccact gcgtggcgat gatagaaagc accagcgtgg tttttccggt
     2761 tcccggaggg ccattaacag caaggatatc gccatgtctt gcatccagaa aatggcttaa
     2821 ggcatcgcgc tgagcctttg ccagcggaaa cttatctccg gagtgtccaa gcctgtcgct
     2881 gaattttgct cctggggcca gcaaagactc tgcagcatga acctctcgcg aggcgaagcg
     2941 attgaagagc ggcacatcct tattgcaaac aagcaggtga tcataaagag aaaggatatg
     3001 ggaactggca ccgccagatt gagccgtttt aacaatataa ccgtgctcag cgagttcata
     3061 ttgctcaggt ttttcaatcc agtcgccggc aacgctcttc agtagcctct ctgagtcata
     3121 cagatattga cgccactcct gctgcaaggc ggcatatcgt gcttcccgtt cttcatccgt
     3181 ttcggcagtc ttatcaacgc tgtcatcaaa gttgatagag aacgtggtat gggtcgtctt
     3241 gtatttgtca tactgcccaa tctcaccaat cgaaaatgct ccttttggca aaggttcaag
     3301 caggtctctg ggaatggagg tcgctggcgt cggatataaa aaaccctcac ggcttagcaa
     3361 ggctggcgtc actatcgggg taacaatatc aggcgcgcct gcggaacggt ccttaccatg
     3421 ctgcagtaac cggaaataaa cttttgggcg caagatgaca tcgaccgttt cgacatcgtc
     3481 tttttctccc tcaaaaaatt tactgacaat cgcttcgtca agacgtcccg ccgctatccc
     3541 atgccagtga gtgaaatttt gggcgtcttt ccgtttaaaa ctgccctttc ctgactcagc
     3601 atccgcaagc gagttgcgcc agtatgaggc aaaccctaaa gcattttcat ccattaccag
     3661 tcctgtgact caatgaaatc cattgattta tcgaatataa aatcaccgta tggacagcta
     3721 ttgtccatat tgaaaaagtt ggatgcaatg ttttgtttat aattcgatat caaatgaact
     3781 ggttgtacaa ggttcagaac acaagctcat taaacagtac gtcaggacta ttgatttcat
     3841 aacagtgaat acatttttta ccgtttataa taactacctg tgttggacgg ggataccaga
     3901 gcagccgaaa acaccggggg ttattacctg ctctcatgga aaccatatat tcctggtgct
     3961 aatcacagtc ataaatagcc tgaagaacgt gacgaaggga ctttaatgga agccggagta
     4021 acttgtcaga aacatcaatc ttcgtcatca tttacaaaga aaaat
//
LOCUS       DAEBXN010000230         4042 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.232, whole genome shotgun sequence.
ACCESSION   DAEBXN010000230 DAEBXN010000000
VERSION     DAEBXN010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.232"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(886..1077)
                     /locus_tag="KJL99_004282"
     CDS             complement(886..1077)
                     /locus_tag="KJL99_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HBD4438774.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLYDH
                     ADELYRSCLARFGDDGENR"
     gene            1142..1465
                     /locus_tag="KJL99_004283"
     CDS             1142..1465
                     /locus_tag="KJL99_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HBD4438775.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPT
                     LAPPLLRQLAAIGNNLNQAARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            1407..2165
                     /locus_tag="KJL99_004284"
                     /pseudo
     CDS             1407..2165
                     /locus_tag="KJL99_004284"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2143..2673
                     /locus_tag="KJL99_004285"
     CDS             2143..2673
                     /locus_tag="KJL99_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HBD4438776.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLSDEFRKLEESVSKAVTSN
                     ETKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTV
                     SGGTLWYLGGMIQANLEEIRKQNESLEKLNAKTWGVRYHEGSNGRFLVLPEGMKAETN
                     WTMENGKQNAVRLVQE"
     gene            2680..2913
                     /locus_tag="KJL99_004286"
     CDS             2680..2913
                     /locus_tag="KJL99_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HBD4438777.1"
                     /translation="MTELESHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVLHLSEQTERLSQLYSENWR"
     gene            complement(3459..3851)
                     /locus_tag="KJL99_004287"
     CDS             complement(3459..3851)
                     /locus_tag="KJL99_004287"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438778.1"
                     /translation="MALSACFRQQLNPVVNSLLKERIIHSPKELEHDNAVIRVLQKLQ
                     IQEVCNSTLWETAKQQLLQKSDGRYFAINSKHLAFPGPGESHAFCCIKYKNAIGINGN
                     NAETQSTHYQPYPYDKVSIWGPFPHNLT"
BASE COUNT          965 a          918 c         1207 g          952 t
ORIGIN      
        1 aaaaaaacca ccgctaccaa cggtggtttg tttgccggaa tctagagcta ccaactcttt
       61 cttccgaagg taactggctt ggtggagcac agataccaaa tgctgtcctt ctagtgtagc
      121 cgcagttagg ccaccacttc aagaactctt aatatctcaa tccaccttgt ccagttacca
      181 atggctgctg ccagtggcgt taagtcgtgt cttaccgggt tggactcaag acgatagtta
      241 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
      301 cgaacgacct acaccgaact gagataccaa cagcgtgagc tatgagaaag cgccacgctt
      361 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
      421 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
      481 ctctgacttg agcgtcggtt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
      541 gccatcggcg cggccttctt cctgttcctg gtcttttgct cacatgttct ttccggcgtt
      601 atcccctgat tctgtggata acagcatttc gtttttgagt gagtagatac cgctcgccgc
      661 agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag cggaaaagcg cctggacgtg
      721 cattttctcc ttacgcatct gtgcggcatt tcacacccgg tatggcgcac ttttcataca
      781 atccgcactg atgccgcatg gttaagccag tatacactcc gctatcgcta cgtgactggg
      841 tcatggctgc gccccgacac ccgctaacac ccgctgacaa gcccgtcagc ggttttcacc
      901 gtcatccccg aagcgagcga ggcaactgcg gtaaagctca tcggcgtggt cgtaaagcga
      961 ttcacagatg tctgcctgtt catccgcgtc cagttcgttg agcttctcca gcagcgttaa
     1021 tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga
     1081 tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgcgagag
     1141 gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gctgtgaggg
     1201 taaacagctg gcggtatgga tgcggcgggt ctgcctgggt gagccggttg cccgttccgg
     1261 gaggctgccg acactggcac cgccgttact gcgtcagctg gccgccatcg ggaataacct
     1321 gaatcaggca gcgaggaagg tgaacagcgg gcagtggtct tccggtgacc gggttcaggt
     1381 ggtggccgca ctgatggcca tcggggatga gctgcgccgg ctgcgtctgg ccgtcaggga
     1441 acaggggacg cgggatgata gttaagtttc atgccagggg aaaaggcggt ggcagtggtc
     1501 cggttgatta cctgctggga agggagcgta accgggaagg ggcaacggtg cttcagggta
     1561 atccggaaga agtccgggaa ctcatcgatg ccacgccatt tgcgaagaaa tacacgtccg
     1621 gtgttctctc gttcgcggag aaggagctgc cgccgggagg gcgtgaaaaa gtgatggcga
     1681 gctttgagcg tgtactgatg cccggtctcg aaaaaaatca gtacagcatt ctgtgggtgg
     1741 agcaccagga caaggggcgg cttgagctga attttgtcat tccgaacatg gagctacaga
     1801 ccggaaaacg cctccagccg tactacgacc gcgcagacag gccgagaatt gatgcctggc
     1861 agacgctggt gaatcaccat tacgggctgc atgacccgaa cgccccggag aaccgccgga
     1921 cgctgactct ccctgataac ctgccggaaa cgaaacaggc gcttgctgag agcattacac
     1981 ggtggataga cgctctttat catgtcgggg agataaaagg ccgtcaggat gtgattcagg
     2041 cgctcacaga ggcggggctt gaagtggtca gggtgacgcg aagcagtatc agcattgcag
     2101 acccgaacgg cgggaagaat atcagactga aaggagcatt ctatgagcaa tcttttacag
     2161 acgggcgcgg agttcgagaa aaagctgaaa gagagagccg aatctacaga gaaaatgctg
     2221 agcgacgagt tcaggaagct cgaagaatct gtaagcaagg ctgtgacatc aaacgagacg
     2281 aaaatcaaag acgctatagc cctgttcaca gcttcgacag aggaatcact gaaaaaacat
     2341 cgggaagggg tgaaagaggc gatgatgcag cacaggaagg acgtgttaaa gctggcaggg
     2401 aatacgggca tgatgttact ggggatggtc attttcctgt ttaccgtgag tggcgggacg
     2461 ctctggtatc ttggcgggat gatacaggcg aacctggaag aaatcaggaa gcagaacgag
     2521 agtctggaga agctgaacgc gaagacatgg ggcgtgcgtt atcacgaggg gagcaacgga
     2581 cgttttctgg tgttgcccga ggggatgaaa gccgaaacga actggacgat ggaaaacggg
     2641 aaacagaatg ccgtaaggct ggtacaggag tgacagagca tgacggagct ggaaagccat
     2701 ttgctgaacg cattagagca gctgcaacag gactatatgc agcggctgaa cgaatgggag
     2761 agcgccttcg tggaattgca gaaaatgttt tcgcttacgc aacgggacaa cgcgatgctg
     2821 aacgagcggg tcatgcagtt gagtcagcag gtgctgcact tgagcgagca gacagaacgc
     2881 ttgagccagt tatacagcga gaactggaga taagagagga acggttgata caggagcgcg
     2941 aacatgcgtt attcctggaa cgggaacgcc agccggaaat acaggaacgg acactggatg
     3001 gcccttcgct gggatggtaa tcagagacag gctaaaatgt tgtcagcgcg tttccggtgc
     3061 attttttggg ataatggttg gttcgggggc accgggaacg cgcttcagtc agaccttgct
     3121 gaaaacacag gaacgagtta aaacgcatac gggcgcgatt ctggtgtgtc ttttgtcgct
     3181 gtgttttgcc tctattgcca gatcgcgctc attcccctga acttctgttt tcccgctcag
     3241 gtccccgtct tcttcgcttc cgaaagcatg aataaaatcc gtttcagctg tctctggctg
     3301 gcaattcctg cgtgattcac atggctgcat agctatgcat gtggttaaaa tttaccgggt
     3361 gcgatcgcgg cagtttttcg ggtggtttgt tgccattttt acctgtctgc tgccgtgatc
     3421 gcgccgaacg cgtttcagcg gtgcgtacaa ttaagatatt acgttaaatt gtgagggaat
     3481 ggtccccata tactgacttt atcataggga tagggttggt agtgggtcga ttgggtttcg
     3541 gcattgtttc cgttaatgcc aattgcattt ttgtatttga tgcaacagaa ggcgtgagat
     3601 tctcctgggc ctggaaaggc taagtgttta gaattgatgg caaaatatct tccgtccgat
     3661 ttttgaagta gttgttgttt ggctgtctcc caaagggtgc tgttacaaac ttcttgaatt
     3721 tgtagttttt gcaggactct tattactgcg ttgtcatgtt ccaattcttt aggggagtgt
     3781 attattctct cttttaataa gctgtttacc acagggttta gttgttgtct aaagcacgca
     3841 gataatgcca ttgctacaca attttgttcc cgaggaagca tgtttggcgg ggtatgccgt
     3901 gtttgatgtg atatgtctat tctcatagaa ggtctctggc ttatagcatc aacaaatacg
     3961 gctataaaac aaaattttca tagagtgatt caagatcttc ttgagatcct tttttgcggc
     4021 gcgtaatctg ctgcctgcaa ac
//
LOCUS       DAEBXN010000231         3989 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.233, whole genome shotgun sequence.
ACCESSION   DAEBXN010000231 DAEBXN010000000
VERSION     DAEBXN010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3989
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.233"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(336..1985)
                     /gene="pgi"
                     /locus_tag="KJL99_004288"
     CDS             complement(336..1985)
                     /gene="pgi"
                     /locus_tag="KJL99_004288"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HBD4438779.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHISEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            2510..3859
                     /gene="lysC"
                     /locus_tag="KJL99_004289"
     CDS             2510..3859
                     /gene="lysC"
                     /locus_tag="KJL99_004289"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HBD4438780.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
BASE COUNT          945 a          989 c         1040 g         1015 t
ORIGIN      
        1 atgaatatca aatataagtt tcaacttata tttaaataac aataaaaatc ataagcttaa
       61 aataaaaaac aaaagtaatt aatctgcgta ttctccgaaa taaacaacga ttaaaaatct
      121 attttaggat taacctcacg gtatgatttc cgttaaatca cagacaaggg gatttatctg
      181 ataaaacaat gccgacttat tgtcggcatt gtttttaagg ttatttaact cctcatacta
      241 tgcattaatg tcgatattgg catcaggctc cggttgccgg atgcggcgta aacgccttat
      301 ccggcctaca tatcggcatc aggcacgcat gatgattaac cgcgccacgc tttatagcgg
      361 ttaatcagac cattggtcga gctatcgtgg ctgctgattt ctttatcatc tttcagctct
      421 ggcagaatac ggtttgccag ttgtttaccc agctccacgc cccactggtc gaaggtgaag
      481 atgttcagga tcacgccctg agtaaagatt ttgtgctcat acagcgcaat caacgcaccc
      541 aggctgaacg gagtgatttc gcgcagcagg atggagttgg tcgggcggtt accttcgaac
      601 actttgaacg gcaccacgta gtcaagcgtt gccggatctt taccctgatc gcgatattcc
      661 tgctcaacca cttcgcggga tttaccaaac gccagcgctt cggtctgggc gaagaagtta
      721 gacagcagtt tctggtggtg atcagagagc gggttatggg tgatagccgg ggcgatgaaa
      781 tcgcacggta ccattttggt tccctggtgg atcagctggt agaacgcgtg ctgaccgtta
      841 gtgcctggtt caccccagat aatcgggcca gtctggtaat ccacaacgtt accgttacgg
      901 tcaacatact taccgttgga ctccatattg ccctgctgga agtacgccgc gaaacggtgc
      961 atatactggt catacggcag aatcgcttca gtttccgcac caaagaaatt gttgtaccag
     1021 atgccaatca gcgccagcag gacaggcagg tttttctcgg caggcgtggt ggagaaatgc
     1081 ttgtccattg cgtgtgcgcc agaaagcagt tcaacgaagt tatcaaagcc gatggagaga
     1141 acaatcgaca ggccaatcgc tgaccacaaa gagtaacggc cgccgaccca gtcccagaac
     1201 tcgaacatgt tggcagtatc aataccaaac tcgccaacgg ctttggcatt ggttgagagc
     1261 gccgcaaaat gtttcgcaac gtgtttttca tcacctgccg ctttcaggaa ccagtcacgc
     1321 gcgctatggg cgttggtcat ggtttcctga gtggtgaagg ttttagatgc taccaggaac
     1381 agcgtggttt ccgggtttac ttttttcagc acttccgaga tgtgagtccc atcgacgtta
     1441 gaaacaaagt gcatgttcag gtggtttttg tacggacgca gtgcttcggt caccatgtac
     1501 gggccgaggt cagaaccgcc gatcccgatg ttcactacgt cagtgattgc tttgccggta
     1561 taacccttcc actcaccgga gataatcgct tccgagaagg ttttcatctt ctccagcacc
     1621 gcgttgactt ctggcataac atctttgcca tcaaccagaa tcggggtatt gctacgatta
     1681 cgcagcgcta cgtgcagcac ggcgcggttt tcagtgcggt tgatcttctc gccagagaac
     1741 atcgacttaa tcgcgcccgc cagatcgcac tctttcgcca gatcctgtaa tttcgccagc
     1801 gtctcttcag tgatgcggtt tttggagtaa tccaccagca tctgatcgtc gaaggttgcg
     1861 gagaacttag aaaaacgatc gccgtcttta gcaaaaagat cggcgatcgt aacgtctttc
     1921 atttcatcga agtgtttctg tagtgcctgc caggcagcgg tctgcgttgg attgatgttt
     1981 ttcattagca atactcttct gattttgaga attgtgactt tggaagattg tagcgccagt
     2041 cacagaaaaa tgtgatggtt ttagtgccgt tagtgtaatg ttgagtgtaa acccttagcg
     2101 cagtgaagca tttattagct gaactactga ccgccaggag tggatgaaaa atccgcatga
     2161 ccccgtcgtt gacaaccgcc ccgctcaccc tttatttata aatgtactac ctgcgctagc
     2221 gcaggccaga agaggcgcgt tgcccaagta acggtgttgg aggagccagt cctgtgataa
     2281 cacctgaggg ggtgcatcgc cgaggtgatt gaacggctgg ccacgttcat catcggctac
     2341 aggggctgaa tcccctgggt tgtcaccaga agcgttcgca gtcgggcgtt tcgcaagtgg
     2401 tggagcactt ctgggtgaaa atagtagcga agtatcgctc tgcgcccacc cgtcttccgc
     2461 tcttcccttg tgccaaggct gaaaatggat cccctgacac gaggtagtta tgtctgaaat
     2521 tgttgtctcc aaatttggcg gtaccagcgt agctgatttt gacgccatga accgcagcgc
     2581 tgatattgtg ctttctgatg ccaacgtgcg tttagtagtc ctctcggctt ctgctggtat
     2641 cactaatctg ctggtcgctt tagcggaagg gctggaacct ggcgagcgat tcgaaaaact
     2701 cgacgctatt cgcaacatcc agtttgccat tctggaacgt ctgcgttacc cgaacgttat
     2761 ccgtgaagag attgaacgtc tgctggagaa cattactgtt ctggcagaag cggcggcgct
     2821 ggcaacgtct ccggcgctga cagatgagct ggtcagccac ggcgagctga tgtcgaccct
     2881 gctgtttgtc gaaatcctgc gcgaacgcga tgttcaggca cagtggtttg atgtacgtaa
     2941 agtgatgcgt accaacgacc gatttggtcg tgcagagcca gatgtagccg cgctggcgga
     3001 actggccgcg ctgcagctgc tcccacgcct caatgaaggc ttagtgatca cccagggatt
     3061 tatcggtagc gaaaataaag gtcgtacaac gacgcttggc cgtggaggca gcgattatac
     3121 ggcagccttg ctggcggagg ctttacacgc atctcgtgtt gatatctgga ccgacgtccc
     3181 gggcatctac accaccgatc cacgcgtagt ttccgcagca aaacgcattg atgaaatcgc
     3241 gtttgccgaa gcggcagaga tggcaacttt tggtgcaaaa gtactgcatc cggcaacgtt
     3301 gctacccgca gtacgcagcg atatcccggt ctttgtcggc tccagcaaag acccacgcgc
     3361 aggtggtacg ctggtgtgca ataaaactga aaatccgccg ctgttccgcg cgctggcgct
     3421 tcgtcgcaat cagactctgc tcactttgca cagcctgaat atgctgcatt ctcgcggctt
     3481 cctcgcggaa gttttcggca tcctcgcgcg gcataatatt tcggtagact taatcaccac
     3541 gtcagaagtg agcgtggcat taacccttga taccaccggt tcaacctcca ctggcgatac
     3601 gttgctgacg caatctctgc tgatggagct ttccgcactg tgccgggtgg aggtggaaga
     3661 aggtctggcg ctggtcgcgt tgattggcaa tgacctgtca aaagcctgcg gcgttggcaa
     3721 agaggtattc ggcgtactgg aaccgttcaa cattcgcatg atttgttacg gcgcatccag
     3781 ccataacctg tgcttcctgg tgcccggcga agatgccgag caggtggtgc aaaaactgca
     3841 tagtaatttg tttgagtaaa tactgtatgg cctggaagct atatttcggg ccgtattgat
     3901 tttcttgtca ctatgaggta gcctgagttt aacggacact ccttcctgaa atagaatggc
     3961 atcagaagga gctaataatg agcagaaaa
//
LOCUS       DAEBXN010000232         3948 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.234, whole genome shotgun sequence.
ACCESSION   DAEBXN010000232 DAEBXN010000000
VERSION     DAEBXN010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3948
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.234"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(<1..785)
                     /locus_tag="KJL99_004290"
     CDS             complement(<1..785)
                     /locus_tag="KJL99_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HBD4438781.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMHYVPSIFGEDRHTSRSKRY
                     AYIPTITVLESLQQEGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWV"
     gene            complement(940..1098)
                     /locus_tag="KJL99_004291"
     CDS             complement(940..1098)
                     /locus_tag="KJL99_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001550547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HBD4438782.1"
                     /translation="MPGCTSRLLPEGPFSREQAVAVKTAYRNVFTEDDQGTYSRLVIR
                     NAEGQLRW"
     gene            complement(1169..1387)
                     /locus_tag="KJL99_004292"
                     /pseudo
     CDS             complement(1169..1387)
                     /locus_tag="KJL99_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
     gene            1582..3123
                     /gene="istA"
                     /locus_tag="KJL99_004293"
     CDS             1582..3123
                     /gene="istA"
                     /locus_tag="KJL99_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc12 family transposase"
                     /protein_id="HBD4438783.1"
                     /translation="MPTVPISMRKLKEILRLKYGVGLSHRQIGRSLAISPSVVSRYAN
                     RAAQLGIKQWPLPTGWDDTKLKHAFLQTQVKMKKHSLPDWATVHRELRNKCVTLQLLW
                     EEYCERNPGGFYSYNHYCRMYREWLKTTSPSMRQVHKAGEKLFVDYCGPTVGVTDPET
                     GEIRTAQVIVAVLGASSYTWAEATWSQQLEDWVMSHVRCFQWLGGVPELVVPDNLKSA
                     TSRACKYDPDVNPTYQQMLEHYNVAVLPARPRKPKDKAKAEVGVQVVERWIMARIRHE
                     IFYSLASLNQRIRELLERLNNKIMQKLGYSRAELFIQLDKPALKPLPEASYSYTLVKK
                     VRVHADYHVEIDKHYYSVPCSLLGQQLEAWISGELVRLFNQGQEVAVHPRKRTYGYST
                     RNEHMPEAHRQHATWTPERLLEWAGHIGSETHSYVLHILNSRPHPEQSYRFCLGLLNL
                     HKKYSKARLNAACARALKTKVWRLSGIKSILEKGLDKQPVQDPKPDLLSTMEHENVRG
                     SEYYH"
     gene            3138..3884
                     /gene="istB"
                     /locus_tag="KJL99_004294"
     CDS             3138..3884
                     /gene="istB"
                     /locus_tag="KJL99_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc12 family helper ATPase
                     IstB"
                     /protein_id="HBD4438784.1"
                     /translation="MNHLYEQLTALKLTGFRDALKKQLAQPGTYQELGFEERLSLLTA
                     EELTCRENRKAERLIKHARFRLNAELSKLDYRNNRGLDRALIRSLSQGNWLTLKQNIL
                     LTGATGSGKTFLACALGHNACRQGYKVYYYRLKALMEQCYQGHADGRYSKLLTRLNNS
                     DLLLLDDWGLEPLSSEQRSDLLEIVDLMYQRGSIIVVSQLPVENWYKMIGDSTHADAI
                     LDRLVHGSIKIELKGESMRKIQSPLTEGDQ"
BASE COUNT         1032 a          977 c          994 g          945 t
ORIGIN      
        1 acccacaatg cgcggttgag cttaatatcg gtgtcgatgt tgtgaatggc acgggtgtgg
       61 atacgttttc cttttgcact gcggccggaa atcccgcctt tcagcatatt ctcctggatg
      121 gtctgatatg cgctccacag gtctttaccg taatcctccc tgcgtcgtgg tgtcagaatg
      181 tcagcggtgg tgactggctg atgttcgtca ccataacggt aagtcagtgc cgcctgtgcc
      241 agcgcctggc gtgccggtgg cggcaggacc agcgactgca tggcatcacg cttttcctca
      301 atccggtcaa aaacccccac cacttcgtaa gccccttcga taactttctc caccacattt
      361 ccccggtgtg gaacacgcac ttcccccaga gactgaccgc agacgcagcc attctggcag
      421 acgaatctga agtaacccgg cagcatctgg tagctggagg taccgtcatg agagttgagc
      481 agaataattt cagggacatg ttctccgttt atctctccgg cccgccgcag acgcagcatg
      541 tgttttgtgt atccccggcg gcccgggtcg cgcacgcggg tctggcaggc gaagaatggc
      601 tgaaaacctt cctgctgcag gctttccagt acggtgatgg tgggaatgta cgcatatcgt
      661 ttactgcggg aagtatgccg gtcttcaccg aaaatactgg gaacatagtg catcagttct
      721 tcgtgtgtca gcggacggtc acggcgtatc tggttcgcat aaccaaaacg actggctagt
      781 cgcataattt gctccttatc ggtggttacg atttactggt gtaataaatg aaaaagctgc
      841 gtctcccgga gaagacgcgg cctgacagat gaaatgaatg acgtttattg tctgagaagc
      901 ccttaactgg cgagctgagt attaagctgt gttccggcat caccagcgca actgaccttc
      961 agcattacgg ataaccagcc gggaatatgt tccctggtca tcttcagtaa acacattgcg
     1021 gtaagctgtt ttgacggcaa cagcctgttc gcgtgagaaa gggccttcgg gcaataaacg
     1081 tgatgtacaa cccggcatat ctgttgctcc ctgaaagtaa aaagccccgg tcatgatgac
     1141 cggggcctga aggagagtga cctgattatc aaaaagtcac attcagcgtg gcctgaccgt
     1201 tataaccttc agcgctgctg ccgctgacgc tgtgggcata accggcctga acgcccaggg
     1261 tgatattttc ccggacacgg gcttccagtc cggcctgcag gtccagtgac gtgccattcc
     1321 gggacggtga gaacgtcatg ttactgccgg cggcggctgt ccccatgctc atgtcacccc
     1381 gggagctgtg cgtattttca tgaaaggaga tcactcaata acttccatcg agatcgggta
     1441 ataacatttg aacagatcgc tgaataacat cgatggagat cacttttgac tcattttgtt
     1501 attcagtgat ctccatcaat gttattggaa cttcacaggt gtgttgatct gtatcttttg
     1561 ccattccggt aaaggatacc tatgccaaca gttccaattt ctatgagaaa acttaaagaa
     1621 attcttaggc ttaaatacgg tgttggactc agccatcgac aaattggtcg tagtcttgca
     1681 atctcccctt ccgttgtatc cagatatgct aatcgggcgg ctcaacttgg cataaagcag
     1741 tggcccttac ctacaggatg ggatgataca aaactaaaac atgcgttcct tcagacccag
     1801 gttaagatga agaagcactc tctgcctgac tgggctacag tacaccggga actgcgtaat
     1861 aaatgcgtga cgctgcagct actctgggaa gaatactgtg agcgtaatcc aggcggtttt
     1921 tacagctata accattactg ccggatgtac cgtgaatggc tcaaaaccac ttcaccatca
     1981 atgcgtcagg tacataaagc tggcgaaaaa cttttcgttg attactgtgg acctaccgtt
     2041 ggcgttaccg accctgagac cggagaaata agaactgctc aggtcatcgt agctgttctc
     2101 ggggcatcaa gttacacatg ggcagaggcc acctggtctc agcagcttga agactgggtg
     2161 atgagtcatg ttcgctgctt ccagtggttg ggtggcgttc ctgaacttgt tgttccggac
     2221 aatctgaaaa gcgccacatc cagggcatgt aagtatgatc ctgacgttaa ccctacctac
     2281 cagcagatgc ttgagcatta taatgtcgca gttttgcctg cgcggccacg taaaccgaaa
     2341 gataaagcca aagctgaagt tggcgttcag gttgttgaac gctggatcat ggcccgaatc
     2401 aggcatgaga tcttctacag ccttgcatcg cttaatcagc gcattcggga gttgctggaa
     2461 agactgaata acaaaataat gcagaagttg ggttattcac gtgcagaact cttcatccag
     2521 cttgataaac ccgcactgaa gcctcttcct gaagccagtt acagttacac cctggtgaag
     2581 aaagtcagag ttcatgccga ttaccacgtg gaaatcgaca aacattacta ctcggttcca
     2641 tgttcgctgt taggccagca actggaagca tggatctccg gagaactggt aagactcttc
     2701 aatcaggggc aggaggttgc tgtgcacccg cgcaagcgta cttatggcta cagtacccgc
     2761 aacgagcaca tgcctgaagc tcatcgacag catgccacct ggacgccaga gcgtcttctg
     2821 gaatgggcgg ggcacatagg cagtgaaact catagttatg tgcttcatat actgaactct
     2881 cgtccacatc cggaacaaag ctatcgcttc tgccttggac tcctgaacct tcataaaaaa
     2941 tacagtaaag ccagacttaa tgcagcatgt gcaagagctc tgaaaacaaa ggtatggcgt
     3001 ctgtcaggta ttaaatcgat cctggaaaaa ggtctggata aacaacctgt tcaggatcca
     3061 aaaccagatc tgttatccac gatggaacac gaaaacgtac gcggcagtga gtattaccac
     3121 tgatacggga tccaatgatg aatcatcttt acgaacaact gaccgcactt aaactcaccg
     3181 gcttccgtga tgcgcttaaa aagcaacttg ctcagccggg cacataccag gagctgggct
     3241 tcgaagaacg cctgtcatta ctgacagcag aagaactaac ctgccgtgaa aacaggaagg
     3301 cagagcgtct gatcaaacat gcacggttca gacttaatgc tgagttatca aagctggatt
     3361 atcgtaacaa tagagggctg gacagggccc tcatccgttc actcagtcag ggaaactggt
     3421 taaccctgaa acaaaatatt ttactgaccg gggccaccgg cagcggtaaa acgttcctgg
     3481 catgtgcact tggtcataat gcctgccgac agggatacaa ggtctactat tatcgcctta
     3541 aagcgctgat ggaacagtgc tatcaggggc atgctgatgg aagatacagc aaacttttga
     3601 ccaggctgaa taatagcgat ctgctgcttc tggatgactg ggggctggaa cctctctcat
     3661 cagaacagcg tagcgacctg ctggaaatag tggatctgat gtaccaacga ggctcaatca
     3721 tcgtagtgag ccagttgccg gtggaaaact ggtacaaaat gatcggagac tccacacatg
     3781 cggatgccat cctagatcga ctggttcatg gcagtatcaa gatcgaactt aaaggagaat
     3841 caatgcggaa aatacaatct ccgttgaccg aaggagatca gtgaaggtaa tttaaaaacg
     3901 gttctgtgaa agtgacacga accgatctcc atcgatgtta ctcaccga
//
LOCUS       DAEBXN010000233         3883 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.236, whole genome shotgun sequence.
ACCESSION   DAEBXN010000233 DAEBXN010000000
VERSION     DAEBXN010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3883
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.236"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(279..482)
                     /locus_tag="KJL99_004295"
     CDS             complement(279..482)
                     /locus_tag="KJL99_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438785.1"
                     /translation="MNETELKHVIALLLEDAKRLQQLEPNAGTGARIWLAKEALESGD
                     YDSEEAFYKAEGRAGYSPGLGGV"
     gene            complement(484..639)
                     /locus_tag="KJL99_004296"
     CDS             complement(484..639)
                     /locus_tag="KJL99_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HBD4438786.1"
                     /translation="MNTIDLGNNESPVYGVFPNQDGTFTAITYTKSKTFKTEAGARRW
                     LGRHSGE"
     gene            complement(842..1249)
                     /locus_tag="KJL99_004297"
     CDS             complement(842..1249)
                     /locus_tag="KJL99_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBD4438787.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            1326..1553
                     /locus_tag="KJL99_004298"
     CDS             1326..1553
                     /locus_tag="KJL99_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBD4438788.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRPGKVIHENQA"
     gene            1537..1959
                     /locus_tag="KJL99_004299"
     CDS             1537..1959
                     /locus_tag="KJL99_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438789.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELSLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSPGDSVVVH"
     gene            2037..2814
                     /locus_tag="KJL99_004300"
                     /pseudo
     CDS             2037..2814
                     /locus_tag="KJL99_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2821..3567
                     /locus_tag="KJL99_004301"
                     /pseudo
     CDS             2821..3567
                     /locus_tag="KJL99_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            3582..>3883
                     /locus_tag="KJL99_004302"
     CDS             3582..>3883
                     /locus_tag="KJL99_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HBD4438790.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPDLIWSLP"
BASE COUNT          997 a          849 c         1061 g          976 t
ORIGIN      
        1 taatgggtta agtgataaca gatgtctgga aatatagggg caaatccact gagccagagg
       61 attacgaagg gcttcataaa tatatgctct cactaggatt caggagaacg atcccccatg
      121 gagatggttc gtataatcaa ctcccggacg gaacttatgt ttccgaaaag ggcggtgata
      181 tttatcaaat tcgcagccag atatctgact atgcagaccg actatccggg tatcgcgcgt
      241 ctgtttttgt ttgcgaattc agtcaatgcg catggtattt ataccccgcc aagacccggt
      301 gaatatcctg cacggccttc tgctttgtag aaggcttctt cgctatcata atcgccagat
      361 tccagcgctt ctttagccag ccagatgcgt gccccagtgc ctgcatttgg ctccagttgc
      421 tggaggcgtt ttgcatcttc taggagtaga gcgataacgt gttttaattc tgtctcgttc
      481 attttactca cctgaatgtc ttcccaacca acgacgtgcg ccagcttcgg ttttaaacgt
      541 tttgcttttg gtatacgtta tggcggtgaa tgtgccgtcc tggttgggga acacgccgta
      601 caccggagat tcgttgttgc caagatcgat agtattcatg ttgaccccat ttccccttaa
      661 cgccggggta gcggaactgt ttgctgagaa caccgtgcgg tgtcttgatg aataaaattt
      721 agaataacct aaggagggtg atcaagattt ttgtgtagaa aaacctaagt tttttgatgt
      781 aaaaaacaca agtgtttgaa agtttgtgct ttttattaca gggtgtggag aaaaaagggg
      841 attatttgtt tgcgcttctt ttgcgagctt tgagtagttc ttcaaaaagt ttgttgaaat
      901 tttcaactcg agcacgcatc tctgacaaca gggccttttg ctctgactca ggcagtgcgt
      961 cgaacagttg aagtaactct ttttgatctt ctgtcagaat ggctggctga ttatccggga
     1021 tcggttcgcc tggttgttta tcttcatccc caaaaagaag ccaagtcggt gagcactgaa
     1081 gcgcttggct cagtgcgaat aatcttttcc ccgccggctg tgtttcatct ctttcccatt
     1141 gagaaattgt tacgtgagcc actttgacca gcttacctaa tgcggcctga gacagtttta
     1201 attttttacg cctatgtaag aggcgagcac cgaaggtttc gtttttcata ttagggaatt
     1261 ctaatttttc ttgacttagg tttctctacg gcctggtttc cttaggaaaa tctaaggagc
     1321 tcgatatgtt gaaaattgat gctatagcgt tttttggcag caaaacaaag cttgccaatg
     1381 ccgcaggagt taggctggca agcattgctg catgggggga actggttcct gaaggtcgcg
     1441 cgatgcgcct gcaagaggca tctggcgggg aacttcagta cgaccccaaa gtttatgacg
     1501 aatatcgtaa ggcaaaacga cctgggaagg tgattcatga aaatcaggca tgagcacatc
     1561 gaatcagtgc tgttagccct ggcagccgaa aaagggcagg cgtgggtcgc taacgcaatt
     1621 actgaagaat atctgcggca ggggggcggc gaattgtctc tggtaccagg caaggactgg
     1681 aacaatcagc agaatatcta tcaccgttgg ttgaaaggtg aaacgaaagc gcaaagggaa
     1741 aaaattcaga aactgatccc tgcggttctg gcaattcttc cgcgtgagct gcgtcaccga
     1801 ctctgtatct tcgataccct ggaacgccgt gcattactgg cggcgcagga agcgttgagt
     1861 acggcaattg atgcgcatga tgatgctgtc caggccgttt accggaaagc acatttcagc
     1921 ggtggtgggt cgcccggcga ttctgtcgta gtgcattgat tgaaattaat cgtgccggat
     1981 tgttttgttc ggtatcagtt aaatgtaacg ctgcgagcgt tacaaggtga aaacaaatgg
     2041 cttcaaactg gataaagctc gaggttatta cgccggataa gccggaaata ttcaggcttg
     2101 ctgagattct gaatattgat ccagatgccg cattagggaa ggttattcgc ttctgggcat
     2161 gggcggatca acaaatgata gacggtaatg cagattgtaa cgctcgcggc gttacaaaaa
     2221 gtgcaataga tcgcatcact tttatgtctg gttttgctga tgcgttaatt caggttggat
     2281 ggctggtcga aaatgacggt gggctttctc tacctaactt tgaacgtcat aacggaaaaa
     2341 gctctaaaaa acgggcggtt acaaacgagc gagtaacaaa aatacgcgaa ctgaaacgaa
     2401 aaggtaacgc tgccagcgtt acacaaacgg atcaaaaagc gttaccagtg gaagaggaag
     2461 aggaagatct aaatactgat ctccccctaa atccccctcg ccaaaaacga gcgtctaaaa
     2521 aattcgagcc ggaggctatt gagctgcccg attggttgcc ggaaacactc tggcatgagt
     2581 gggtccggtt cagacaggca ttgcgaaaac cgattcgaac ggagcagggc gatacgggaa
     2641 ctggaaaaat tccgtcagca gggttttaca cctgagcagg tgattcgaca cagcatcgcc
     2701 aatgaatacc agggcctgtt cgcgccgaaa ggtgttcggc ctgagacgtt gctccgacag
     2761 gttaacaccg tctcgttgcc ggacagtgcg atcccgccag gcttcagggg gtaacggacc
     2821 atgaaaaata ttgcgacagg cggcgttctg gaacgcatcc gccgactgac cccgcaacat
     2881 gtaaccgccc cattcagaac ggtagcggag tggcgcgagt ggcaacttgc tgaaggccag
     2941 aaacgtagcg aggagatcaa ccgcctgaat cgccagttgc gggtggaaaa aattctgaat
     3001 cgctcaggca tccagccgtt gcaccgtaaa tgctcgtttg cgaattacca ggtgcagaac
     3061 gacggccagc gatacgcgtt aagccaggcg aaatccatcg ccgatgaact gatgaccggg
     3121 tgtacaaatt ttgcgttcag cggaaaacct gataccgggt agaatcactt agcggcagct
     3181 atcgggaatc gcctgctgaa agacggtcag acagtgattg tggttaccgt ggctgatgtt
     3241 atgagcgccc tgcacgccag ctatgacgac gggcagtcag gcgaaaaatt tttgcgggag
     3301 ctgtgcgaag tggatctgct ggttcttgat gaaattggca ttcagcgcga gacgaaaaac
     3361 gagcaggtgg tgctgcacca gattgttgat cgccggacag cgtcgatgcg cagcgtgggg
     3421 atgctgacaa atattaacca tgcagcgatg aatacacttc tcggcgagcg ggtgatggat
     3481 cgcatggtca tgaacggcgg gcgctgggtg aattttaact gggagagctg gcgtccgaat
     3541 gttagccatt cgagggttgt taagtagttt caggaggatt tatggcgaaa ccttttactc
     3601 ccgaacagcg ggaagaactg aagacgcgaa ttgtggaact cgtgcatcag gacggtcggg
     3661 tcacgattcg gcagttgtcc gatgaaacag gtatcagtcg tgcgtctgtc ggtcgcttat
     3721 gcatagaact ggtcgcaagt ggtgatgtat ataattctgg ctacggctta ttcccgtctg
     3781 aacaggctcg caaggactgg caaagcgccc gcaaaaaact ctcgagagta aaggtgagga
     3841 aaccggttgt tgttgatccg gaccttatct ggtcattacc tga
//
LOCUS       DAEBXN010000234         3869 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.237, whole genome shotgun sequence.
ACCESSION   DAEBXN010000234 DAEBXN010000000
VERSION     DAEBXN010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3869
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.237"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            109..1443
                     /gene="adeQ"
                     /locus_tag="KJL99_004303"
                     /gene_synonym="yicO"
     CDS             109..1443
                     /gene="adeQ"
                     /locus_tag="KJL99_004303"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HBD4438791.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            1496..1948
                     /locus_tag="KJL99_004304"
     CDS             1496..1948
                     /locus_tag="KJL99_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HBD4438792.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDDSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            2159..3349
                     /gene="nepI"
                     /locus_tag="KJL99_004305"
     CDS             2159..3349
                     /gene="nepI"
                     /locus_tag="KJL99_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HBD4438793.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(3390..3683)
                     /locus_tag="KJL99_004306"
     CDS             complement(3390..3683)
                     /locus_tag="KJL99_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HBD4438794.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPHASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
BASE COUNT          797 a          862 c         1022 g         1188 t
ORIGIN      
        1 aaagaatatc tattaatgtg caattgaaat gtgataatta tcactgaacc gttgttgtca
       61 tttgttttac aaaagcaagg gattaaatct cctccaatgg acaaaaaaat gaataatgac
      121 aataccgatt acgtgagtaa tgaatcaggg acgctttcgc gattatttaa actacctcag
      181 catgggacca ccgtccgcac agaattgatt gcggggatga ccactttttt aaccatggtg
      241 tacattgttt ttgtgaaccc gcaaatcctc ggcgcggcac aaatggaccc gaaagtggtg
      301 tttgttacca cctgtttgat tgccggtatc ggcagtattg cgatggggat atttgctaac
      361 ttacccgtgg cgctggctcc ggcaatgggg ctgaacgcct tctttgcgtt cgtggtcgtg
      421 ggggcgatgg ggatctcctg gcagaccggg atgggcgcga tattctgggg cgcaattggg
      481 ctatttttgc tcacgctgtt tcgtatccgg tactggatga tctccaacat tccattaagt
      541 ttacgtattg gtatcaccag tggaatcgga ttatttattg ccttaatggg attaaaaaac
      601 actggtgtta ttgtcgccaa taaagacacg ctggtgatga ttggcgattt aagttctcac
      661 ggcgtgttgt taggtatttt agggtttttt attataaccg tgttgtcatc acgtcatttt
      721 catgccgcgg tgctggtttc tattgtggtg acgtcttgct gtggattatt tttcggtgat
      781 gttcatttta gcggcgtcta ttccattccg cctgatatta gcggcgtcat tggtgaagta
      841 gatttgagcg gcgcgttaac acttgaactc gccggtatca ttttctcctt tatgctgatc
      901 aacctatttg attcatcagg aacattaatt ggtgtaactg ataaagcggg cttaatagat
      961 agcaacggta aattccccaa tatgaataag gcgctgtatg ttgatagcgt cagttcggtg
     1021 gcaggcgcat ttatcggtac ctcgtctgtt actgcctata ttgaaagtac ttctggtgtg
     1081 gcagtcggtg gtcgcacggg gctaacggct gtcgtggtcg gcgttatgtt cctgttagtt
     1141 atgttcttct caccgctggt ggcgatggtt cctccttacg caaccgctgg agcgttaatc
     1201 tttgttggcg tgctgatgac ttcgagcctg gcgcgcgtta actgggatga ttttaccgaa
     1261 tcggtgcctg cgtttattac cacggtgatg atgcccttta ctttctcgat caccgaaggg
     1321 attgcactcg gctttatgtc gtactgcatc atgaaagtgt gcaccgggcg ctggcgcgat
     1381 ctgaacctgt gtgtggtggt ggtcgcagct ctgttcgcat tgaagattat tctggtggat
     1441 tagtaactgg cgagggtttc cagatatcat gagttctgat tacgcaggag aactcatgat
     1501 ctggataatg ctcgccacgc tggcggtagt gtttgtggtt ggttttcggg tgctgacatc
     1561 cggggccaga aaagcgattc gccgtctcag cgatcggctg aatatcgatg tcgtacccgt
     1621 ggagtcgatg gtcgatcaaa tgggaaagtc agccggtgac gaatttttac gttatttgca
     1681 tcgcccggat gagtcgcacc tgcaaaacgc cgcgcaggtg ttgcttatct ggcaaattgt
     1741 cattgtcgat gatagcgaac agaacctgct gcaatggcac cggattttac aaaaagcccg
     1801 cctttccgcg ccgattaccg atgctcaggt caggctggcg ctgggttttc tgcgtgaaac
     1861 cgaacctgaa atgcaggata ttaatgcttt tcagatgcgc tataacgcgt tctttcagcc
     1921 tgccgagggc gttcactggt tgcattgaaa ccgtttcgtc ttatcagcct acagaccgcg
     1981 cctgacctgt cgggtggata agacgttcac gccgcatccg gtaatctttg ccctgttccg
     2041 tttcgatctt aactccagct caagcaacac cgtaaaagtt gcaaaaacgt taaattcgtc
     2101 acacatttca atgttaaagt ggtcggcttt tcccctgaaa catgccacgg gtaacaccat
     2161 gagtgaattt attgccgaaa accgcggcgc ggatgccatc acccgaccga actggtcagc
     2221 cgttttctcg gtggcgtttt gtgtcgcctg tctgattatc gttgagtttt tgcccgtcag
     2281 tttgttgacg ccaatggccc aggatttagg catttcggaa ggggttgccg ggcaatcggt
     2341 gaccgtgacc gcctttgtgg caatgtttgc cagtttgttt attacccaga caattcaggc
     2401 cactgaccgc cgctacgttg ttattttgtt tgccgttttg ctgacgctct cctgcttgct
     2461 ggtttccttt gctaactcat tcagtttgct tttaatcggt cgtgcctgtc tggggctggc
     2521 gctgggcggg ttctgggcga tgtcggcgtc gctgaccatg cgtctggtac cgccgcgtac
     2581 ggtgccgaag gcgctgtcgg tgatcttcgg cgcggtttct attgcgctgg tgattgccgc
     2641 gccgttgggc agttttttag gcgagcttat cggttggcgc aatgtcttta atgcggcggc
     2701 ggtgatgggc gtgctgtgta ttttctggat tatcaaatca ttgccttcac tgccaggcga
     2761 accctcgcat cagaaacaaa atactttccg cttattacaa cgtccgggtg tgatggcagg
     2821 gatgatcgcc atcttcatgt ctttcgccgg gcagtttgct ttcttcacgt atattcgccc
     2881 ggtgtatatg aacctggcgg gattcggcgt ggatggctta acgctggtgc tgttgagttt
     2941 tggtatcgcc agctttattg gtacgtcgct ttcgtcgttc attcttaaac gttcggtaaa
     3001 actggcctta gcaggcgcgc cgttaatact ggctgtgagt gcgttggtac tgacgttgtg
     3061 gggaagcgat aaaatcgttg ctaccggcgt ggcgattatc tgggggctaa cttttgcatt
     3121 ggttcccgtc ggctggtcaa cgtggatcac ccgctcgctg gccgatcagg cagaaaaagc
     3181 cgggtctatt caggtggcgg ttattcagct tgctaatacc tgtggcgcgg caatcggcgg
     3241 ttatgcgctg gataatattg gtctgacttc gccgttgatg ttgtccggca cattgatgtt
     3301 gctgactgca ttgttggtta ctgcaaaggt gaaaatgaag aaatcctgat atcaggtaaa
     3361 gaagcgttgg tcagtagttg ttgctgttct tacatatccg ggcattctac cttacccaac
     3421 gcttcccaat aattgctctg attattacgt tccatcgcca cgatggcctc gcgtatatgt
     3481 tgtttattgt tttcatctga caacattaac ttttcccacg cttcatctga taatgacgca
     3541 tgtggcgtgc aaattttacg taaatcttgt aataccgtgg ttttctcttt tgcggactgc
     3601 agtgagctaa aaggcgactc ggcgtatacg atgcctggca tagcgaaaaa agtgaaaata
     3661 agcagtagtg tcgttggttt catggattca accttttaca aatgtgtatc gaatgaagca
     3721 taagtgtagt ttgctttcat cgctctcatc atgctgtacg aaacgcgctt gcgtggtcag
     3781 taagaagtgc cagactttat attccacttt tattcctttt tattcttatc gatagcgttt
     3841 cgttttttaa accgcagcga ccttaccgc
//
LOCUS       DAEBXN010000235         3834 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.238, whole genome shotgun sequence.
ACCESSION   DAEBXN010000235 DAEBXN010000000
VERSION     DAEBXN010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3834
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.238"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(79..870)
                     /gene="aadA1"
                     /locus_tag="KJL99_004307"
     CDS             complement(79..870)
                     /gene="aadA1"
                     /locus_tag="KJL99_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HBD4438795.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            complement(983..1813)
                     /locus_tag="KJL99_004308"
     CDS             complement(983..1813)
                     /locus_tag="KJL99_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HBD4438796.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     gene            2023..3036
                     /gene="intI1"
                     /locus_tag="KJL99_004309"
     CDS             2023..3036
                     /gene="intI1"
                     /locus_tag="KJL99_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HBD4438797.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            3239..3589
                     /locus_tag="KJL99_004310"
     CDS             3239..3589
                     /locus_tag="KJL99_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HBD4438798.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            3715..>3834
                     /locus_tag="KJL99_004311"
     CDS             3715..>3834
                     /locus_tag="KJL99_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBD4438799.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDV"
BASE COUNT          839 a          985 c         1004 g         1006 t
ORIGIN      
        1 aattatgtgc ttagtgcatc taacgcttga gttaagccgc gccgcgaagc ggcgtcggct
       61 tgaacgaatt gttagacatt atttgccgac taccttggtg atctcgcctt tcacgtagtg
      121 aacaaattct tccaactgat ctgcgcgcga ggccaagcga tcttcttctt gtccaagata
      181 agcctgtcta gcttcaagta tgacgggctg atactgggcc ggcaggcgct ccattgccca
      241 gtcggcagcg acatccttcg gcgcgatttt gccggttact gcgctgtacc aaatgcggga
      301 caacgtaagc actacatttc gctcatcgcc agcccagtcg ggcggcgagt tccatagcgt
      361 taaggtttca tttagcgcct caaatagatc ctgttcagga accggatcaa agagttcctc
      421 cgccgctgga cctaccaagg caacgctatg ttctcttgct tttgtcagca agatagccag
      481 atcaatgtcg atcgtggctg gctcgaagat acctgcaaga atgtcattgc gctgccattc
      541 tccaaattgc agttcgcgct tagctggata acgccacgga atgatgtcgt cgtgcacaac
      601 aatggtgact tctacagcgc ggagaatctc gctctctcca ggggaagccg aagtttccaa
      661 aaggtcgttg atcaaagctc gccgcgttgt ttcatcaagc cttacggtca ccgtaaccag
      721 caaatcaata tcactgtgtg gcttcaggcc gccatccact gcggagccgt acaaatgtac
      781 ggccagcaac gtcggttcga gatggcgctc gatgacgcca actacctctg atagttgagt
      841 cgatacttcg gcgatcacca cttccctcat gatgtttaac gtttgaaata aggggcgccg
      901 agcgccagcg aggggagcca aaagcttgct tttggccgtc ccgacttgat tgaagggttg
      961 ggcgattttg ccattagatt ttttataaat ttagtgtgtt tagaatggtg atcgcatttt
     1021 tcttggcttt tatgcttgat gttaaattcg accccaagtt tcctgtaagt gcggacacaa
     1081 aaacatattt atgtcctgat ttgcttataa taaacccttc aaaccatccg ttttgtaagg
     1141 ttctatttgc tgtgaatcct gcaccagttt tcccatacag ttttgtacta ttatccagat
     1201 cttgtagata catgttctct atggtgtttt ctatggctga gtttttaact gggagattgt
     1261 gattaataat tttacgcagg aattgaattt gttcttctgg tgaaattttt aagctacttt
     1321 cgagccatgc ttctgttaat ccgttgtttc tttctttatc tccagagaag tcttgatttc
     1381 cataatcaaa atctttgaga taattcttga ttttatttaa tccaattttt tgggttattt
     1441 cttgcgaaac ccaaacaaca gaaaattgca tccacgtctt tggtgtatga ttgctgttcc
     1501 agatctccat tcctttgggg gttttatccc atttgaatat ggttttctga tctattattt
     1561 ccgcatcaaa tgccataagt gataatgcga tcttgaaagt tgaatctggt gccatttgcg
     1621 ttgcacactt tgctttattg aattgagcaa tttcagcgtt tgtggatgca tcgtaaagta
     1681 aaaaacaacc ttcagttcct tcaaataatg gagatgcaac agtagagata tctgttgatg
     1741 cactggcgct gctgtagata atatttgcaa ttattaaaaa aatagcgaag ttgatatgta
     1801 ttgtgttttt cataataagt attggtttgg taaagggctt aattttaacg gctaacaatt
     1861 aatgaggctc cgggttcgcc caactttgtt ttagggcgac tgccctgctg cgtaacatcg
     1921 ttgctgctcc ataacatcaa acatcgaccc acggcgtaac gcgcttgctg cttggatgcc
     1981 cgaggcatag actgtacccc aaaaaaacag tcataacaag ccatgaaaac cgccactgcg
     2041 ccgttaccac cgctgcgttc ggtcaaggtt ctggaccagt tgcgtgagcg catacgctac
     2101 ttgcattaca gcttacgaac cgaacaggct tatgtccact gggttcgtgc cttcatccgt
     2161 ttccacggtg tgcgtcaccc ggcaaccttg ggcagcagcg aagtcgaggc atttctgtcc
     2221 tggctggcga acgagcgcaa ggtttcggtc tccacgcatc gtcaggcatt ggcggccttg
     2281 ctgttcttct acggcaaggt gctgtgcacg gatctgccct ggcttcagga gatcggaaga
     2341 cctcggccgt cgcggcgctt gccggtggtg ctgaccccgg atgaagtggt tcgcatcctc
     2401 ggttttctgg aaggcgagca tcgtttgttc gcccagcttc tgtatggaac gggcatgcgg
     2461 atcagtgagg gtttgcaact gcgggtcaag gatctggatt tcgatcacgg cacgatcatc
     2521 gtgcgggagg gcaagggctc caaggatcgg gccttgatgt tacccgagag cttggcaccc
     2581 agcctgcgcg agcagctgtc gcgtgcacgg gcatggtggc tgaaggacca ggccgagggc
     2641 cgcagcggcg ttgcgcttcc cgacgccctt gagcggaagt atccgcgcgc cgggcattcc
     2701 tggccgtggt tctgggtttt tgcgcagcac acgcattcga ccgatccacg gagcggtgtc
     2761 gtgcgtcgcc atcacatgta tgaccagacc tttcagcgcg ccttcaaacg tgccgtagaa
     2821 caagcaggca tcacgaagcc cgccacaccg cacaccctcc gccactcgtt cgcgacggcc
     2881 ttgctccgca gcggttacga cattcgaacc gtgcaggatc tgctcggcca ttccgacgtc
     2941 tctacgacga tgatttacac gcatgtgctg aaagttggcg gtgccggagt gcgctcaccg
     3001 cttgatgcgc tgccgcccct cactagtgag aggtagggca gcgcaagtca atcctggcgg
     3061 attcactacc cctgcgcgaa ggccatcggt gccgcatcga acggccggtt gcggaaagtc
     3121 ctccctgcgt ccgctgatgg ccggcagcag cccgtcgttg cctgatggat ccaacccctc
     3181 cgctgctata gtgcagtcgg cttctgacgt tcagtgcagc cgtcttctga aaacgacaat
     3241 ggaggtggta gccgagggtg tggaaacacc cgactgcctt gcgtggttgc ggcaggcggg
     3301 ttgcgacacg gtgcagggtt tcctgttcgc caggccgatg ccggcggcgg ccttcgtcgg
     3361 cttcgtcaac caatggagga acaccaccat gaacgccaat gaaccgagca ccagttgctg
     3421 cgtgtgctgc aaggaaatcc cgctcgatgc cgccttcacg ccggaagggg ccgagtacgt
     3481 ggagcatttc tgcgggctgg agtgctatca gcgcttccag gcgcgggcca gcactgcgac
     3541 cgaaaccagc gtcaaaccgg acgcttgtga ttcgccgccg tcaggttgag gcatacccta
     3601 acctgatgtc agatgccatg tgtaaattgc gtcaggatag gattgaattt tgaatttatt
     3661 gacatatctc gttgaaggtc atagagtctt ccctgacatt ttgcagggaa ttccatgact
     3721 ggacagcgca ttgggtatat cagggtcagc accttcgacc agaacccgga acggcaactg
     3781 gaaggcgtca aggttgatcg cgcttttagc gacaaggcat ccggcaagga tgtc
//
LOCUS       DAEBXN010000236         3806 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.239, whole genome shotgun sequence.
ACCESSION   DAEBXN010000236 DAEBXN010000000
VERSION     DAEBXN010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3806
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.239"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            437..2089
                     /locus_tag="KJL99_004312"
     CDS             437..2089
                     /locus_tag="KJL99_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HBD4438800.1"
                     /translation="MIPLKKNITLIMFTLSLLTGNPAIAYETDKVYKITVLHTNDHHG
                     HFWRNNHGEYGLSSQKTLVDNIRQKVINNGGSVLLLSGGDINTGVPESDLQKAEPDIR
                     GMNLIGYDAMAVGNHEFDNPLNILRQQEKWATFPFLSANIYQKSTGRRLFSPWKIFIR
                     QNLKIAVIGLTTDDTAKTGNSEYFTDIEFRQPAAEARSVIDELNQQEKPDIIIAATHM
                     GHYDNGESGSNAPGDVEMARSLPTGSLAMIVGGHSQAPVCMASDNKKQWNYIPGTTCV
                     PDKQNGIWIVQAHEWGKYVGQADFEFCNGTMKLVNYQLHPVNLKMRITREDGKTEFSF
                     YTPEITEDPQMLSLLTPFQNKGKAQLDVKVGVVNGRLEGDRSKVRFVQTSMGHLILSA
                     LTERIDADFAVVSGGEIRDSIESGNITYKDILKVQPFGNTVVSIDLTGKEVADYLATV
                     AQMKPDSGAYPQFLNTSFVVKKGKIEMLKIKGKSVDLNKKYRMTTFSFNATGGDGYPR
                     IDNRPGYINTGFIDAEVLIEYIRKHSPLDAASYEPKGEVSWQ"
     gene            complement(2100..2270)
                     /locus_tag="KJL99_004313"
                     /pseudo
     CDS             complement(2100..2270)
                     /locus_tag="KJL99_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2403..2522)
                     /locus_tag="KJL99_004314"
                     /pseudo
     CDS             complement(2403..2522)
                     /locus_tag="KJL99_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2596..3526)
                     /locus_tag="KJL99_004315"
                     /pseudo
     CDS             complement(2596..3526)
                     /locus_tag="KJL99_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001766279.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT         1052 a          878 c          874 g         1002 t
ORIGIN      
        1 aatcgacgat tgctggaaag gctgggccac atccctccgg cagaagcaga aaaagcttat
       61 tatgcttcta tcggaaacga tgatctggca gcctgagttc acagataaaa cattctccag
      121 gaaacttggg gcggttcacg tcagtatcag accatattaa ttgctgaaac tgataaacat
      181 caaaccttat agaagggtga atgctgaaag ggggctatgg tgatcgacgt gatgacaacg
      241 acaggtaaga ccgtgacctg ataaacctga attgtgtcct agacataaag tccgtgaagg
      301 gaataaggct caggtcagcg aatcttatag tggcccgcag ctctgactgc aataaggccg
      361 gatacagagc agcaactact cgtcgatgtc agaacaacgg aagccttgca aacggggatg
      421 cgttcataca ggaacaatga taccgttaaa aaaaaacatt acgctaataa tgttcaccct
      481 gtcattactg acaggaaatc ctgctatagc gtatgaaaca gataaagtat ataaaataac
      541 cgttctgcat acaaatgacc accatggaca cttttggcgt aacaatcatg gtgagtacgg
      601 gttatcctcc caaaaaactc tggtggacaa tatccggcag aaagtaataa ataatggcgg
      661 aagtgttcta ttattatctg gtggtgatat taacaccggt gtacctgaat cagaccttca
      721 gaaggctgaa cccgatattc gtggaatgaa tctaattggt tatgatgcaa tggccgtcgg
      781 taatcatgaa tttgataatc ctctaaatat attacgtcag caggaaaaat gggccacatt
      841 tcctttcctt tctgctaaca tatatcaaaa aagtacaggg aggcgtttat tcagtccatg
      901 gaaaattttc atccgtcaga atttgaaaat tgcagtcatt ggcttgacga cagatgatac
      961 tgcgaaaaca ggaaattctg aatattttac tgatattgag ttccgtcaac ctgctgctga
     1021 ggcgaggagt gtcattgatg aacttaatca gcaggaaaag ccagatatta ttattgcggc
     1081 aacacatatg ggtcactatg acaatggcga gtcaggttcg aatgctcctg gtgatgttga
     1141 aatggctcgc agtttgccga ctggctcatt agcaatgatt gtgggaggac attcacaagc
     1201 tcctgtctgc atggccagcg ataataaaaa acagtggaat tatattccgg ggacaacctg
     1261 tgtaccagat aaacaaaatg gcatctggat tgtacaagcg catgagtggg gaaaatatgt
     1321 cggtcaggct gattttgaat tctgtaacgg tacaatgaaa ctggttaatt atcagttaca
     1381 cccagttaat ctcaaaatga gaattaccag agaagatgga aaaacggaat tttcttttta
     1441 cacccccgaa attactgaag atccacagat gttatcctta ctgacaccat tccagaataa
     1501 aggcaaagca caactggatg tgaaggttgg ggttgtgaac ggacgtctgg aaggggacag
     1561 aagtaaagtt cgttttgttc agaccagtat gggccatctg attctgagcg ctctgacaga
     1621 acgtattgac gcagattttg ctgttgtcag tggtggggaa attcgagatt ccatagagag
     1681 tggtaacatc acatacaaag atatactgaa agttcagcca tttggtaata cggtagtaag
     1741 tattgatttg acggggaaag aagtagctga ctacctagct acagtggcac agatgaaacc
     1801 ggattccgga gcctacccac aatttttgaa taccagcttt gtagtcaaaa aaggaaaaat
     1861 cgaaatgcta aaaataaaag gtaaatcggt tgaccttaac aaaaaatatc gtatgacaac
     1921 atttagcttt aatgctactg gtggggatgg ctatccacgt attgataata gacccggata
     1981 cataaatacc ggatttattg atgctgaagt acttatcgaa tatatccgga aacattcacc
     2041 gctggatgct gcttcatatg aaccaaaagg tgaagtcagt tggcagtgat aacgtaattg
     2101 acgatcatca cgtgtttttt gctgacagtc gctgtgactt tgtgaaagta gtctctgcgt
     2161 atgtttgcga tatgggaatg cagttgctgt attttgtgtt tctgcttctg ccagttgttg
     2221 ctgaatttaa ccttgcggct taactgatgt tggagcgctg ccagcttctt cgtcaatttc
     2281 gctgattaaa gtttattcat tatggcatga tctgcgtaag cggctcgtca gaaccatatt
     2341 gatatttact aagagctcag atcaactttc cagggcaaca gatcgcgtac ccggtttgcc
     2401 ggtcagtcct ggatatgctc aatgacgtaa cgcagccatt tttctggctc cacattgttc
     2461 agacggcatg tgccgatcag cgagtacaac accgccgcat gttcgccacc gctatcggaa
     2521 ccccgccgga gagagtttca ctctccggct gctcacggaa ccgtacgtga cactctcgcg
     2581 tcatacggct cttgatcact cattcgatgc acgtagtaaa taccagtggg caaatagctt
     2641 cggatactgt ttccttacca gcgccagctt tcgctgagcc ttccggttcg aaagtctgta
     2701 tttagactgc atccacttaa gcaaacgtga ctgcattgca ctccacagtc gatagctgaa
     2761 gtttctggac cagaactttc catagtactc aatccagcct ctcactatcg cattgtagcg
     2821 cctcgcaaaa tccagcaaac tctcagctgt tgagcgatgt atacgccact tcttgattgt
     2881 ttctgttatt ttgcacattg ctgcatttga tgcacccggc atacattttc tgtacaattc
     2941 gcctttgaaa ttcttcagag tacgcacttt gaagtcatat ccgagagtgg catacaacat
     3001 catcggcgta cctttcgaag ggaacgccgg gaaactgacg ttgcatccac atatcaaagg
     3061 catagtgcag gaacagattt gagagcaggg gggagattac gccgccctgc ggcgttcctc
     3121 gaccccgtgc cagcattgct ccatccggca tttgcaccgg tgccttcagc ctgcgctcaa
     3181 tgtacatcac cacccagcgt gcctgagtat gatgctgcac tgtcttcagt agcaggtcat
     3241 gatcgatatc atcaacgaag cctttaatat cgacttccac cacccagtca aacttccagc
     3301 agcgtttcct cgcctgcgca atcgcctggt gtgcggattt acccggtcga taaccatacg
     3361 agtctgcatg gaacaggggt tccagtaccg gctcgagtat ctgcttaacg accatctggg
     3421 cgatccggtc cgacacggcc ggaataccga gcggacgtac accaccgtca gctttgggaa
     3481 tgtccacacg ccttaccgcc tgcggcatat aactgccgga agccattctg ttccacagtt
     3541 tgtagagatt gtctgtaaca ttctcgtcaa acccggccag tgtctgcccg tcaatgcctg
     3601 ccgccccacg gttggctttt actcgtttat aagcttccca tacctgtcgt ttacttacag
     3661 gaaatggctt tgccgatttt acagcagtcc tcccatcggc attgtcgccc gtggttgccg
     3721 ccgcaccatc agcacaatgg ggcaagccct ttgctccgga cccattacgg tccttcaccg
     3781 ctactacggt ttactccgtc cctgta
//
LOCUS       DAEBXN010000237         3796 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.240, whole genome shotgun sequence.
ACCESSION   DAEBXN010000237 DAEBXN010000000
VERSION     DAEBXN010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3796
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.240"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..746
                     /locus_tag="KJL99_004316"
     CDS             <1..746
                     /locus_tag="KJL99_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBD4438801.1"
                     /translation="VDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWN
                     NVTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIAT
                     LGSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKL
                     SFYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPL
                     GLSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            1080..3359
                     /gene="ydeP"
                     /locus_tag="KJL99_004317"
     CDS             1080..3359
                     /gene="ydeP"
                     /locus_tag="KJL99_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HBD4438802.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKNSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFPPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            3607..>3796
                     /gene="safA"
                     /locus_tag="KJL99_004318"
     CDS             3607..>3796
                     /gene="safA"
                     /locus_tag="KJL99_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HBD4438803.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSL"
BASE COUNT         1042 a          850 c          899 g         1005 t
ORIGIN      
        1 ttgtagactt gtctcagcat ataagttgct ggaatgatta cggcggctgg tacgacactg
       61 atcatataaa cctggtacaa ggctcagctt tcgcaggctc cctacagtca tataaaggat
      121 cgctgtactg gaataatgtg acctacccct tcccgttgac gacaaatacc aacgtattgg
      181 atattggtga taagacaccg atgccattac cgttaaaact ctatattacc ccagttgggg
      241 cggccggtgg agttgtcata aaagcggggg aagtcattgc ccgtattcat atgtataaaa
      301 ttgcaacatt aggaagcgga aatccgcgta attttacgtg gaatattatt tcaaataaca
      361 gtgttgttat gccaacaggc ggctgcaccg ttgatagtcg taatgtcacc gtggatctcc
      421 cggactttcc tggaagcgca gaaatacccc ttggcgtata ttgctcaagc gaacaaaagc
      481 tctcatttta tctttcgggt gccaccactg acagtagccg acaagtgttt gctaacacag
      541 cacctgatgc aacaaaagca tcgggagtgg gtgtcactct aatgcgtaat ggaaaaatac
      601 tggctacggg agaaaatgtc tccctgggga cagtaaataa aagcaaagta cctctgggtt
      661 taagcgcaac ctatggacaa acaggaaaca aggtttcagc cggaaccgtc cagtcagtca
      721 ttggtgtcac ttttatctat gaataattgt gattttgacc ttagtctatg cgctccattc
      781 acgaaaattg cgcggataat tattttgtga aggctattat cctacacctg taagaaaatc
      841 cgcgcacaat tgaagccaga gtcttatttt ttatattgga aaatatgtta atttaaacac
      901 tatgagagat gatgcttaac ttatcgtatt taatctattg tttaacgata atagattaaa
      961 aatagaaaga gagtagtccc ttactcttat atatccatgt tggcgataat ccctttgtat
     1021 gtactgtgca tcatcgctat tacaaatcct aataattcat ttccacacag gataagtaga
     1081 tgaagaaaaa aattgaatcc taccagggtg ctgcaggtgg ttggggtgct gttaaatccg
     1141 tagcgaatgc agtacgtaag cagatggata tacgccatga tgttattgcc atgtttgaca
     1201 tgaataagcc agagggcttt gactgtccgg gttgtgcatg gccagatcct aagaacagtg
     1261 cgtcattcga catttgtgaa aacggcgcaa aagcaatcgc ttgggaagtc acggataagc
     1321 aggtaaatgc ctctttcttt gctgagaata cggttcaatc attacttacc tggggagatc
     1381 acgagcttga ggctgcgggg cgtctcactc agcctttgaa atatgatgcc gtcagcgact
     1441 gttacaagcc attaagctgg caacaagctt tcgacgaaat tggcgcacgc cttcaaagct
     1501 atagtgatcc caatcaggtt gaattctata cttcgggccg cacttccaat gaagctgcct
     1561 ttctttatca gctttttgcc cgtgaatacg ggagcaataa ctttcccgac tgctccaaca
     1621 tgtgccatga accgacaagc gtgggtttgg cagcgagtat cggtgtaggt aaagggaccg
     1681 tgttgctgga agactttgag aagtgcgatt tagtcatttg cattgggcat aaccctggta
     1741 caaaccaccc tcgcatgctg acttcgttgc gcgctttagt gaaacgggga gcgaaaatga
     1801 tcgccatcaa tcctctacag gaacgtggcc tggagcgatt taccgcaccg caaaacccgt
     1861 ttgaaatgct gacgaactct gagactcagt tggccagtgc ctactacaac gtgcgcattg
     1921 gtggcgatat ggcgttgctc aaggggatga tgcgcctgtt aattgagcgc gatgatgctg
     1981 caagtgccgc aggtcggccc tcattgcttg atgacgaatt tattcaaacg cataccgtcg
     2041 gctttgacga gctacgccgt gacgttctca attccgagtg gaaagatatc gaacgtattt
     2101 ctggactaag tcagacacaa atcgccgaac tggctgacgc atatgccgct gccgaacgca
     2161 ccattatctg ttacggaatg gggatcactc agcacgaaca tggtacccag aacgtgcagc
     2221 aactggtcaa tttgctgttg atgaaaggta acattggcaa gcctggtgcg ggtatctgcc
     2281 cactacgtgg tcactctaat gtacagggcg accgaaccgt cggtatcacc gagaaaccgt
     2341 ctgcagagtt tctggctcgc ctgggtgagc gctatggctt ccccccacct catgcacctg
     2401 gacatgctgc aattgccagc atgcaagcaa tatgtacggg gcaggctcga gcattgatct
     2461 gcatgggggg caactttgca ctggcaatgc cagatcggga agcgagcgct gtaccgttaa
     2521 cgcaattaga tttggcggta cacgtagcca ctaagcttaa ccgctctcat ctgctgaccg
     2581 cacggcatag ctatattctg ccggttctgg ggcgtagcga gattgatatg caaaaaagcg
     2641 gtgcgcaggc ggtaaccgtt gaggattcaa tgtcgatgat tcatgcctcg cgtggcgtgt
     2701 taaaacccgc cggtgtaatg ctgaaatcag agtgtgcagt ggtcgcggga atcgcgcagg
     2761 cagcactacc ccagagcgtg gtagcctggg agtatctggt ggaagattat gatcgcattc
     2821 gcaatgacat tgaagctgtg ctgccagagt tcgccgacta taaccagcgc atccgtcatc
     2881 ccggtggttt tcacctgata aatgcagctg ctgaaaggcg ctggatgacg ccgtcaggta
     2941 aggctaattt cattaccagc aaagggctgt tagaagatcc ctcttcagcg tttaacagta
     3001 agctggtcat ggcgacagta cgcagccacg atcagtacaa cacgacgatt tatggtatgg
     3061 atgatcgcta tcgaggggta ttcggtcaac gagatgtggt ctttatgagt gctaaacaag
     3121 ctaaaatttg ccgtgtaaaa aacggcgaaa gagttaatct tattgcgctt acgccagacg
     3181 gtaagcgcag ctcacgccgc atggatagat taaaagtggt catttaccct atggctgacc
     3241 gctcactggt gacctatttt ccagaatcga atcacatgct aacacttgat aaccacgatc
     3301 cattaagtgg tattcctggc tataaaagta ttccggttga attagaacca tcaaattaat
     3361 gcctcttctc atttcttctg ctgtcatccg cacagcagaa gaattcctca ttgactatta
     3421 ttgcgcaatt tgctcacatg gattaaatta aactacatac tataagatat aaacttctgc
     3481 ctacagctgt aagaaactcc gctcagtact gaagcaccag tcctatttct tcttttctcc
     3541 agcctgttat attaagcata ctgattaacg acttttaacg ttatccgcta aataaacata
     3601 tttgaaatgc atgcgaccac agtgaaaaac aaaatcacgc aaagagataa ctataaagaa
     3661 atcatgtctg taattgtggt tgtcttatta ctgacactta cgttgatagc cattttttcg
     3721 gcaattgatc agctgagtat ttcagaaatg ggtcgcattg caagagatct tacacatttc
     3781 attatcaata gtttgc
//
LOCUS       DAEBXN010000238         3620 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.241, whole genome shotgun sequence.
ACCESSION   DAEBXN010000238 DAEBXN010000000
VERSION     DAEBXN010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3620
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.241"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            90..1241
                     /gene="yiaY"
                     /locus_tag="KJL99_004319"
     CDS             90..1241
                     /gene="yiaY"
                     /locus_tag="KJL99_004319"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HBD4438804.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHNK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVVIYRAAM"
     gene            1406..2944
                     /gene="aldB"
                     /locus_tag="KJL99_004320"
     CDS             1406..2944
                     /gene="aldB"
                     /locus_tag="KJL99_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HBD4438805.1"
                     /translation="MTNNPPSAQIKPGEYGFPIKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(3163..3306)
                     /locus_tag="KJL99_004321"
     CDS             complement(3163..3306)
                     /locus_tag="KJL99_004321"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438806.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRPLTIE
                     TRD"
     gene            3489..>3620
                     /locus_tag="KJL99_004322"
     CDS             3489..>3620
                     /locus_tag="KJL99_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HBD4438807.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     "
BASE COUNT          907 a          860 c          944 g          909 t
ORIGIN      
        1 aattttgata attaataata atgatcatgt tttcgaatga aaatcctcag taagctgccc
       61 accctttttt acactttcag gagtgtgtta tggcatcttc aactttcttt attccttctg
      121 tgaatgtcat cggcgctgat tcattgactg atgcaatgaa tatgatggca gattatggat
      181 ttacccgtac cttaattgtc actgacaata tgttaacgaa attaggtatg gcgggtgatg
      241 tgcaaaaagc actggaagaa cgcaatattt ttagcgttat ttatgatggc acccaaccta
      301 accccaccac ggaaaacgtc gccgcaggtt tgaaattact taaagaaaat aattgcgata
      361 gcgtgatctc cttaggcggt ggttctccac acgactgcgc aaaaggtatt gcgctggtgg
      421 cagccaatgg cggcgatatt cgcgattacg aaggcgttga ccgctctgca aaaccgcagc
      481 tgccgatgat cgccatcaat accacggcgg gtacggcctc tgaaatgacc cgtttctgca
      541 tcatcactga cgaagcgcgt cataacaaaa tggcgattgt tgataagcac gtgactccgc
      601 tgctttctgt caatgactcc tctctgatga ttggtatgcc gaagtcactg accgccgcaa
      661 cgggtatgga tgccttaacg cacgctatcg aagcgtatgt ttctattgcc gccacgccga
      721 tcactgacgc ttgtgcactg aaagccgtga ccatgattgc cgaaaacctg ccgttagccg
      781 ttgaagatgg cagtaatgcg aaagcgcgtg aagcaatggc ttatgcccag ttccttgccg
      841 gtatggcgtt caataatgct tctctgggtt atgttcatgc gatggcgcac cagctgggcg
      901 gtttctacaa cctgccacac ggtgtatgta acgccgttct gctgccgcat gttcaggtat
      961 tcaacagcaa agtcgccgct gcacgtctgc gtgactgtgc cgctgcaatg ggcgtgaacg
     1021 tgacaggtaa aaacgacgcg gaaggtgctg aagcctgcat taacgccatc cgtgaactgg
     1081 cgaagaaagt ggatatcccg gcaggcctac gcgacctgaa cgtgaaagaa gaagatttcg
     1141 cggttctggc gactaatgcc ctgaaagatg cctgtggctt tactaacccg atccaggcaa
     1201 ctcacgaaga aattgtggtg atttatcgcg ccgcaatgta atcatcattc caacaacggc
     1261 tggcaaattg tcagccgctt tttcaactat ctctgtaatc cttgcccgta aattcgtgat
     1321 agctgtcgta aagctgttac cgactggcga agatttcgcc agtcacgtct acccttgtta
     1381 tacctcacac cgcaaggaga cgatcatgac caataatccc ccttcagcac agattaagcc
     1441 cggcgagtat ggtttcccca tcaagttaaa agcccgctac gacaacttta ttggcggtga
     1501 atgggtagcc cctgccgatg gtgagtatta ccagaatctg acgccggtga ccgggcagct
     1561 gctgtgcgaa gtggcgtctt cgggcaaacg agacatcgat ctggcgctgg atgctgcgca
     1621 caaagtgaaa gataaatggg cgcacacctc ggtgcaggat cgcgcggcga ttctgtttaa
     1681 gattgccgat cgaatggaac aaaacctcga gctgttagcg acagctgaaa cctgggataa
     1741 cggcaaaccc attcgcgaaa ccagtgctgc ggatgtaccg ctggcgattg accatttccg
     1801 ctatttcgcc tcgtgtattc gggcgcagga aggtgggatc agtgaagttg atagcgaaac
     1861 cgtggcctat catttccatg aaccgttagg cgtggtgggg cagattatcc cgtggaactt
     1921 cccgctgctg atggcgagct ggaaaatggc tcccgcgctg gcggcgggca actgtgtggt
     1981 gctgaaaccc gcacgtctta ccccgctttc tgtactgctg ctaatggaaa ttgtcggtga
     2041 tttactgccg ccgggcgtgg tgaacgtggt caatggcgca ggtggggaaa ttggcgaata
     2101 tctggcgacc tcgaaacgca tcgccaaagt ggcgtttacc ggctcaacgg aagtgggcca
     2161 acaaattatg caatacgcaa cgcaaaacat tattccggtg acgctggagt tgggcggtaa
     2221 gtcgccaaat atcttctttg ctgatgtgat ggatgaagaa gatgcctttt tcgataaagc
     2281 gctggaaggc tttgcactgt ttgcctttaa ccagggcgaa gtttgcacct gtccgagtcg
     2341 tgccttagtg caggagtcaa tctacgaacg ctttatggaa cgcgccatcc gccgtgtcga
     2401 aagcattcgc agcggtaacc cgctcgacag cgtgacgcaa atgggcgcgc aggtttctca
     2461 cgggcaactg gaaaccatcc tcaactacat tgatatcggt aaaaaagagg gcgctgacgt
     2521 gctcacaggc gggcggcgca agctgctgga aggtgaactg aaagacggct actacctcga
     2581 accgacgatt ctgtttggtc agaacaatat gcgggtgttc caggaggaga tttttggccc
     2641 ggtgctggcg gtgaccacct tcaaaacgat ggaagaagcg ctggagctgg cgaacgatac
     2701 gcaatatggc ctgggcgcgg gcgtctggag ccgcaacggt aatctggcct ataagatggg
     2761 gcgcggcata caggctgggc gcgtgtggac caactgttat cacgcttacc cggcacatgc
     2821 tgcgtttggt ggctacaaac aatcaggtat cggtcgcgaa acccacaaga tgatgctgga
     2881 gcattaccag caaaccaagt gcctgctggt gagctactcg gataaaccgt tggggctgtt
     2941 ctgatataag aagctggtcg cattgggtat tcattgcctg atgcgacgct tacgcgtctt
     3001 atcatgccta cgggaacctg accgtaggcc ggataaggcg tacacgccgc atccggcata
     3061 aacaaagcgc acgttgttac aatttacgtt ttaaacctac gtgcgcaata acccagacta
     3121 cgccaacaga agtgcgtttc tggggcgtgt tttgtcagga gtttaatctc gcgtttcgat
     3181 ggtgagcggg cgaaccaggc cgtaaatcac cattggtgat ccgaggccaa tcagcaataa
     3241 acggtagata attgaaggtt taatacgctt aatctgtact gtggatgttt tactggagtt
     3301 attcattagt ttggccaata gttccactat ttcaatatga taaacctcgt cctgttgaaa
     3361 aaccagacag cacatttcag aaagtatata aaaaacaata tgtcgttata taaataagga
     3421 aattgcactg catcatggca tttcataata tgcgttaatt gttctttttt atattctttg
     3481 agggaaatat gttcctggac tattttgcac tgggagtgct tatttttgta tttctggtga
     3541 tattctatgg gattattatt ttacatgata ttccctacct gattgctaaa aaacgtaatc
     3601 atcctcatgc cgacgccatt
//
LOCUS       DAEBXN010000239         3558 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.242, whole genome shotgun sequence.
ACCESSION   DAEBXN010000239 DAEBXN010000000
VERSION     DAEBXN010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3558
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.242"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            1..308
                     /locus_tag="KJL99_004323"
                     /pseudo
     CDS             1..308
                     /locus_tag="KJL99_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            338..741
                     /locus_tag="KJL99_004324"
                     /pseudo
     CDS             338..741
                     /locus_tag="KJL99_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031967.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            1326..2042
                     /locus_tag="KJL99_004325"
     CDS             1326..2042
                     /locus_tag="KJL99_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438808.1"
                     /translation="MNDNSLLRNSSLFIAYMGCVGWVSAYSYGWGTSFYYGFPWWVVG
                     AGLDDVARSLLYAIIVMGILFTGWGIGILFFLLIKKRSKIQDLSFFRLFFAITLLFFP
                     VIFELLILKQYFILPLSLSFIISSLVISIIIRIYGRIFSVSCFSDIPFVREHRIKLIM
                     AGFLVYFWFFSFLVGWYKPQLKKEYQMLCYNNSWYYVLARYDSRLVLSSSFKDDSNRF
                     LIFNTEQSGFYEINDVYVRK"
     gene            2594..2998
                     /locus_tag="KJL99_004326"
     CDS             2594..2998
                     /locus_tag="KJL99_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438809.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
     gene            3061..3558
                     /locus_tag="KJL99_004327"
                     /pseudo
     CDS             3061..3558
                     /locus_tag="KJL99_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         1004 a          737 c          843 g          974 t
ORIGIN      
        1 tgctgggcgc tgaaagaact ggcaaaggat atctggagca gaccatggag cgaggaaaga
       61 cgcaacgact ggcagagatg gctgcggcca acagtgacgt ccccatgatg aagaatgtgg
      121 ctaaaacgat agggaaaagg ttgtacggta tcctgaacgc catgcgccat ggcgtatcaa
      181 acggaaatgc ggaagcactg aacagcaaga tcaggctgct gagaataaaa gccaagggct
      241 accgcaaccg ggagcgcttt aagctgggag tgatgttcca ctatgggaaa ctgaatatag
      301 cgttctaagc cttcccacca tgatcgggga agccccagat atgaacaggc acattcagag
      361 ttactttaaa gagcttactg aacgtctgag gtgttagagc catgactgcc gcagcattgc
      421 tggcggaaat gcctgaatcg agcagcctct cgagacgtga aatcagcgca cttgtcggtg
      481 ttgctcaggt aaaccgggac tcgggaactc tgcgcggtcg acgaaccatt ttcggtgatt
      541 gtgctgggga agaacagctc tgtacatggc ggcgcttgag gccccccccg gtttaatctg
      601 gtgataaagg cgttttacat gcgcctactt gcagccggaa acgccaaaaa agtggcactg
      661 gttgcctgta tgcgtaagtt gctgaccatt atgaacgcaa tgctcagaaa aaacgaagaa
      721 tggaatgaat cgtatttatg aaatactgga ttgtgggcca taaatattcc aaaaagatag
      781 cccgctccgt ttattaccgg aacgggcgag agcatgacat tgtctgttat tataatgaga
      841 ctgctgattc tacttatttt taatttcaag aaaagcgttt tctacaaaga attatgagat
      901 ttaatgtttt gtttagacaa tattatcatt ctgttcaata atgaaagtct tcatcaacaa
      961 caagtaaaaa caacgtccct ctcatcgaac cggacttgca gatttcccgc atccggctct
     1021 cttgcaacac atcagacctt cacgttcggg cggttatggg gaaggtccag caagctgtat
     1081 gagccctaac tgtttgaata ccagtagatt accagaaact tctatcgatt gaagacatta
     1141 ccaactagcg ttactaaatg ttgaccaagt tttgttcact tgtctggttt tgatagtagc
     1201 attctgcaga gtattttgat atgataaata tgcattaaca atgatattat tgttaagttc
     1261 ctagcatgaa aattaaaaga tcaactttta tctcgaatat tttttacatt atcagttagt
     1321 ttcttatgaa tgacaattca ttgcttagaa attcttcatt attcatcgcc tatatgggat
     1381 gtgtagggtg ggtcagtgct tattcttatg gctggggaac ctctttttac tatggatttc
     1441 catggtgggt tgttggtgca ggtttggatg atgttgccag aagtttactc tatgctatca
     1501 tagtaatggg tatacttttt accggttggg ggattggtat tttatttttt cttcttataa
     1561 agaaaagaag taaaatacaa gacttgagtt tttttagact attttttgct attaccctac
     1621 tgtttttccc tgtaatattc gaacttttaa ttctcaaaca atatttcata cttccattat
     1681 cactttcatt cattattagt tcattggtta tttcaataat aattcgtata tatggacgga
     1741 tcttttctgt atcatgtttt tcagatatcc cttttgttag agaacaccgc attaagttaa
     1801 ttatggctgg ttttttggtt tatttttggt ttttttcctt tcttgttggc tggtataaac
     1861 ctcaattaaa aaaagaatat cagatgcttt gttataataa tagttggtat tatgttcttg
     1921 ctcgttatga ttcacggctc gttttatcta gttcatttaa agatgacagt aacagattct
     1981 taatatttaa tacagaacag tcgggatttt atgaaatcaa tgatgtttat gtcagaaagt
     2041 aataatcctc tcccacaaag tgaattaaat gaatatataa tgcgttcgaa gtatatgtat
     2101 tgagctgtca tactgagaaa caaaccttag ggcagcacaa ttaaagccag aaacaactat
     2161 gcaagaaagt tactaaataa gtgctcggaa cagtaaaaca ctcagggggg actcagtccg
     2221 gattatgtaa tcagataaat tgccaaacat cacaaatcac cagccggact gagtgatgcc
     2281 gagcattgca ccaatacccc gtgatgaacg atgcctgatg cagaaagctc cctataaaac
     2341 aaagaacaaa aactacgacc ggcgattcac cgtcatactg atgctgcatc gtggtgacac
     2401 tgtctgtcac tttcatgttg actgaacaac tctgctgcga agaaggtgta ccgccggcgg
     2461 gatttgattg tccggcgtct agcttccact tccctgatgg aactgaaagt tgttatggcc
     2521 ggtcagcttg ccctgtccgc aggacatgag gtgctgtgac ggacagcacc agtaacggaa
     2581 agtgacaatg aaaatgaata ctgcgctgat agttgcacta atgtgtatgt ggtatgcggt
     2641 acccgctgct gcaaaagaga ctttactggc tatgccacgt aattcaacag aacattgcta
     2701 tgcggagatt aatgtgcacg gaccatatgg ggtgtatttc cgagtggtcc cgcatccccc
     2761 cgggggaaag agctgggtcg aatgtaacag tgactactat tactctgata aaccaccggg
     2821 agtacagata ctcggaaccc gtgcagggtg tcgggtatac ggaatctgcg ggaccacctc
     2881 gacactgcat gtggctggaa gaggggttgt gtgtattaaa aatatatgca gtccacgtgg
     2941 gatgataatt cacaggatac ggaaacgtcc tgtagtggct gtaagcgatg agatgtgatc
     3001 ttacccagca atagtagaca cgcggctaag tgagtaaact ctcagtcaga ggtgactcac
     3061 atgacaaaaa cagtatcaac cagcaaaaaa acacgtaaac agcattcgcc tgaattttgc
     3121 agtgaagccc tgaagcttgc tgaacgcatc ggtgttgctg ccgcagcccg tgaactcagc
     3181 ctgtatgaat ctcagctcta tgcgtggcgc agcaaactgc agcaacagat gacctcgtct
     3241 gagcgtgaaa gtgaactgcc cgcctgaagc ggcaactggc ggagcaggca gaggaactgg
     3301 ctatcctcca aaaggccgcg acatactttg cgcagcggct ggtatacgtg gtgtcagcgt
     3361 cggacaggaa taagccctcg tcagcagttc cgtcagcact gcgacagcgt tgtcctcgcg
     3421 gcggccttta cccggtcaaa acagcgttac ggtgcaccac gcctgacgga tgaactgcgt
     3481 gctcagggtt accactttaa cgtaaaaacc gtggcggcaa gcctgcgccg tcaggggctg
     3541 agggcaaagg cctcccgg
//
LOCUS       DAEBXN010000240         3557 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.243, whole genome shotgun sequence.
ACCESSION   DAEBXN010000240 DAEBXN010000000
VERSION     DAEBXN010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3557
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.243"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..77
                     /locus_tag="KJL99_004328"
     CDS             <1..77
                     /locus_tag="KJL99_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBD4438810.1"
                     /translation="LSFSKSVELHDKVIGHYLNIKHYQ"
     gene            435..1493
                     /gene="rfaQ"
                     /locus_tag="KJL99_004329"
     CDS             435..1493
                     /gene="rfaQ"
                     /locus_tag="KJL99_004329"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HBD4438811.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            1490..2614
                     /locus_tag="KJL99_004330"
     CDS             1490..2614
                     /locus_tag="KJL99_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBD4438812.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFEVIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGADRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            2607..3404
                     /gene="rfaP"
                     /locus_tag="KJL99_004331"
     CDS             2607..3404
                     /gene="rfaP"
                     /locus_tag="KJL99_004331"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HBD4438813.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPGRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
BASE COUNT          947 a          767 c          857 g          986 t
ORIGIN      
        1 cactgtcgtt ctcaaaatcg gtggagctgc atgacaaggt catcgggcat tatctgaaca
       61 taaaacacta tcagtaagtt ggagtcatta cccgtttttg acttaaatag caatttaccc
      121 gccaattctt gttgaacatc tatacttttt ttgtattttt atgcgattca ttgatttctg
      181 ctgtacgtgc aggaaggaat cgcagagcaa cagtgaaaga atatggttgc taatgatttt
      241 atttgattga tttctttatt gaaaatcaac aatgtgctta aaaatcattc gggtcggatt
      301 gccgcgaaag aagtgataca ctagcgcgtc aatatcagtg cggtatcagt attcaggtag
      361 ctgttgagcc tggggcggta gcgtgctttt tctgcttcac cttaatcgga taatctcaac
      421 aaaaagagtt acttgtggat aagccatttc gaagaatttt gctcattaag atgcgttttc
      481 atggggatat gttattaact actcccgtca ttagttctct gaaaaaaaat taccctgacg
      541 caaaaatcga tgtgctgctt tatcaggaca ccatcccgat cctgtctgaa aatccagaga
      601 ttaacgcgct ctacggcata aaaaataaaa aagcaaaagc ctcagaaaaa attgccaact
      661 tttttcatct catcaaggta ttacgtgcca ataagtatga ccttatcgtc aatcttaccg
      721 atcaatggat ggttgctata ctggttcgct tattaaatgc tcgtgtgaaa atttcccagg
      781 attatcatca tcggcagtct gctttttggc gtaaaagttt cacccatttg gtgccgttgc
      841 agggtggaaa tgtggtggaa agtaacttat ccgtgctgac accattggga cttgattcgt
      901 tggtgaagca gacaaccatg agttacccgc ctgcaagctg gaaacgtatg cgtcgcgaac
      961 ttgatcacgc tggtgttgga caaaattatg tggttatcca acctacggcg cggcaaatct
     1021 tcaaatgctg ggacaacgcc aagttttccg ctgtgattga tgccttacat gctcgtggtt
     1081 atgaagtcgt tctgacgtcc ggcccagata aagacgatct ggcctgcgtc aatgaaattg
     1141 cgcagggatg ccagacgcca ccagtaacgg cgctggctgg aaaggtgacc ttcccggaac
     1201 ttggtgcgtt aatcgatcat gcgcagctgt ttattggcgt tgattccgca ccggcgcata
     1261 ttgccgctgc agttaatacg ccgctgatat cgctgtttgg cgcaacagat catattttct
     1321 ggcgtccctg gtcaaataac atgattcaat tctgggcggg agattaccgg gaaatgccaa
     1381 cgcgcgatca gcgtgaccga aatgagatgt atctttcggt tattccggcg gcagatgtca
     1441 ttgctgctgt cgataaatta ctgccctcct ccacgacagg tacgtcgtta tgatcgttgc
     1501 tttttgttta tataaatatt ttccctttgg cggtttgcag cgcgatttta tgcgtattgc
     1561 tcagacagtc gccgcccgag gtcatcatgt tcgggtttat acccagtcgt gggaaggcga
     1621 atgccctgat gtatttgaag tgatcaaagt gccggttaaa tcgcatacca atcacgggcg
     1681 caatgcggag tattttgcct gggtgcaaaa acatttacgc gaacatcccg tcgataaagt
     1741 cgttggattc aacaaaatgc cggggctgga cgtttattat gccgctgatg tttgttatgc
     1801 cgagaaagta gcgcaggaaa aaggcttttt ctatcgcctg acgtcacgtt atcgccatta
     1861 tgccgccttt gagcgggcaa ccttcgaaca gggcaagccg acacagctgc tgatgctgac
     1921 agataagcaa atcgccgatt tccagaaaca ttatcagact gaagcggagc gttttcatat
     1981 tctgccaccg gggatttatc ctgatcgtaa atatagccag cagccagcca atagccgtga
     2041 aatcttccgt aagaagaatg gaataaccga acaacaatat ttattgttgc aggtcggttc
     2101 agacttcacg cgtaaaggtg ccgatcgttc cattgaagca cttgcttcgt taccggattc
     2161 gctgcgccac aacacattgc tatatgttgt tgggcaggat aaaccgcgaa aatttgaggc
     2221 actggcagaa aaacgcggcg tgcgcagtaa tgttcacttc ttctcggggc gcaacgatgt
     2281 ctcggaatta atggcggcgg cggatttatt actgcatcct gcctaccagg aagcggcggg
     2341 aattgtgctg ctggaagcga ttactgcagg attaccggta ctaacaactg ccgtttgtgg
     2401 ctatgcgcat tatattgtcg acgctaattg cggcgaggct attgctgagc cattccgcca
     2461 ggaaacattg aatgagattt tacgcaaagc gttaacgcaa tcttcattgc gccaggcttg
     2521 ggcggaaaat gcgcgacatt atgctgatac acaagattta tacagtctgc cagagaaagc
     2581 ggcggatatc ataacgggtg gtctggatgg ttgaacttaa agagccgttt gccacgttat
     2641 ggcgcggcaa agatcctttt gaggaagtta aaaccttgca gggtgaggta tttcgtgaac
     2701 tggaaactcg ccgtactctg cgctttgaaa tggcgggaaa aagctatttt ctcaaatggc
     2761 atcgcggcac gaccctgaaa gagataatca aaaatttact ctcattgcgg atgccagtat
     2821 taggcgcaga ccgcgaatgg aatgcgattc atcgactgcg ggatgtcggc gttgatacta
     2881 tgtatggggt ggcattcggc gaaaaaggca tgaatccgct gaccagaacc tcgtttatta
     2941 ttaccgaaga tctgacacca accatcagtc tggaagatta ctgtgctgac tgggcgacca
     3001 acccaccaga tgttcgcgta aagcgtatgc ttattaagcg tgtcgcgacg atggtgcgcg
     3061 atatgcatgc tgcgggcatt aaccatcgtg actgttatat ctgtcatttc ctgctgcact
     3121 tgcctttttc cggtaaggaa gaggagttaa aaatttcggt aattgaccta caccgggcgc
     3181 agcttcgcac gcgcgttcca ggtcgttggc gcgataaaga tcttataggg ctttattttt
     3241 cttcaatgaa tattggcctg actcagcggg atatctggcg ctttatgaag gtgtattttg
     3301 ccgccccgct taaagacatt ctcaagcagg aacaaggact gctgtcgcaa gcagaagcaa
     3361 aagccacaaa aatcagggaa agaacgattc gaaaatcgtt gtaacccgta acttattttt
     3421 gccaaaattt tggatacaga ataaatatgt ctcaactcaa tgatagtgac atcatccttt
     3481 ttgagtataa ttttcattat caaaatataa gatctaaaaa tactcttgat atagcatttg
     3541 gtattgacag aaatttt
//
LOCUS       DAEBXN010000241         3548 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.244, whole genome shotgun sequence.
ACCESSION   DAEBXN010000241 DAEBXN010000000
VERSION     DAEBXN010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.244"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..465
                     /gene="pgrR"
                     /locus_tag="KJL99_004332"
     CDS             <1..465
                     /gene="pgrR"
                     /locus_tag="KJL99_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HBD4438814.1"
                     /translation="RLGGEMDKDMIAIRIGPDIPMAIVGSPDYFSRRSAPTSVSQLID
                     HQAINLYLPTSGTANRWRLIRGGREVRVRMEGQLLLNTIDLIIDAAIDGHGLAYLPYD
                     QVERAIKEKKLIRVLDKFTPDLPGYHLYYPHRRHAGSAFSLFIDRLKYKGAV"
     gene            802..2415
                     /gene="mppA"
                     /locus_tag="KJL99_004333"
     CDS             802..2415
                     /gene="mppA"
                     /locus_tag="KJL99_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HBD4438815.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFIPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSMHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(2466..3497)
                     /gene="ynaI"
                     /locus_tag="KJL99_004334"
     CDS             complement(2466..3497)
                     /gene="ynaI"
                     /locus_tag="KJL99_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HBD4438816.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSISNYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
BASE COUNT         1006 a          773 c          819 g          950 t
ORIGIN      
        1 cgtctgggtg gggagatgga taaagatatg atcgccattc gaatcgggcc agatatacca
       61 atggctattg ttggctcacc ggattatttt tctcgtcgaa gtgctccaac gtcagtgtca
      121 caattaatag atcatcaggc aattaatttg tatcttccca catcgggtac agcaaatcgc
      181 tggagattaa tacgcggtgg acgtgaagtt cgtgttcgca tggaaggtca gcttttactg
      241 aatacgatag acctgatcat tgatgctgca attgatgggc atggattggc gtatctacct
      301 tatgatcagg ttgagcgggc tattaaagaa aaaaaactga tacgtgtttt ggataaattc
      361 actccagatt tacccggtta tcacctgtac tatccacacc gtcgacatgc tggctcggca
      421 ttctcattat ttatagatag gctgaagtat aaaggtgctg tttagcacta cttgctgata
      481 cattaattta attcttctct taacgtattc tcagttcctt tcaacgtttt ggttattttt
      541 tattcttcgt acaatggcga cagatgctga ttatgatacc gaaaactggt ttgaacgtgc
      601 gaagcccgaa cgtagtgttc ggtgttctat gtgctttgcc acattttggc gtctattatt
      661 tacactaaat ctgatttgat atattgatac ttaaaacatt tgatgcttcc tttgtcactt
      721 ttttgatgga agttgtttgc atttctttaa ggcgaaacaa ataattacgc atcaatttta
      781 atgtcggtta gagggaaact tatgaagcac tctgtttcag ttacgtgttg tgcgctgttg
      841 gtcagcagca tttctctttc gtatgctgca gaagttccga gcggcacagt actggcagag
      901 aagcaggagc tggtgcgcca cattaaagat gagcctgcgt cgctggatcc cgctaaagcc
      961 gtgggcctgc cagagattca ggtcattcgc gatctgtttg aaggtctggt gaatcagaac
     1021 gaaaaagggg agattgtccc cggcgtcgcg actcaatgga aaagtaatga caaccgtatc
     1081 tggactttta ccctgcgcga taacgcaaaa tgggcggatg gcacaccggt aacggcgcaa
     1141 gattttgtct acagctggca acgtctggtg gacccaaaaa cattgtcgcc atttgcatgg
     1201 tttgccgcgc tggcgggaat caacaacgca caggcgatta ttgatggtaa agctacgcct
     1261 gatcagcttg gcgtcaccgc agttgatgcc catactttga aaattcagct tgataaaccg
     1321 ttgccgtggt ttgtgaattt aaccgctaac tttgccttct tcccggtgca aaaagccaac
     1381 gtagaaagcg gtaaagagtg gacgaaaccc ggaaatctga tcggcaatgg cgcttatgtt
     1441 cttaaagagc gcgtagtcaa tgaaaaactg gtcgtggtac cgaataccca ttattgggat
     1501 aacgccaaaa cggtgctgca aaaagtgacc ttcctgccaa ttaatcagga atccgcagcc
     1561 actaagcgtt acctcgcggg ggatattgat atcaccgaat ccttcccgaa aaatatgtat
     1621 cagaagctgt tgaaggatat tccggggcag gtttatacgc caccgcagct cgggacctat
     1681 tattatgcct ttaacacgca aaaagggccg acggcagatc agcgcgttcg cctggcatta
     1741 agtatgacga tagatcgccg cctgatgacc gaaaaagtgt tggggacggg cgaaaagcca
     1801 gcgtggcatt ttataccaga tgttaccgcc ggatttacgc cggaaccttc gccgtttgaa
     1861 caaatgagtc aggaagaact gaatgcgcag gcaaaaactt tgttgagcgc agctggttat
     1921 ggtccgcaaa aaccgctgaa gctgacgctt ttgtataaca cttcagaaaa ccatcaaaaa
     1981 attgcgattg ctgtagcatc gatgtggaaa aagaaccttg gcgtagatgt taaattgcaa
     2041 aatcaggaat ggaaaaccta tatcgatagc cgtaacaccg gcaattttga tgttatccgc
     2101 gcctcatggg tgggggatta taatgaaccc tccactttcc tgacattatt aacgtcaatg
     2161 cattcaggaa atatttcacg ctttaacaat ccggcatacg acaaagttct ggcccaggca
     2221 tcgacggaaa ataccgttaa agcgcgtaat gccgattaca acgcggcaga aaaaatcctc
     2281 atggagcaag caccgattgc accaatttat caatatacca atggacgatt aatcaaaccg
     2341 tggctgaaag gttatcccat taataaccct gaagatgtag cgtacagtcg gactatgtat
     2401 attgtgaagc attgatgtga tgggaactgg cgttaccctt gtggataacg ccagtgatgt
     2461 ctgatttagc gcccctgttc cggcggtgta atgttatcca tatacagcgt ctggctgggg
     2521 aaggcaaagt ccgcgccgtg tgactgtaca atatcgataa tcttcaaata aacgtcttgc
     2581 tgtgcagcaa gccactcagc ccataccgtg gttttggtaa agcaataaac cataatattc
     2641 aatgacgagt cagcaaactg gttgaaataa accagtaagg tttgtcgctg gtcgatggcc
     2701 gggtgatttt tcagcatctc acgtacggct tcgacaataa cgcccacttt tgccgcatcc
     2761 tcataacgta aaccaatggt cgtggtaatg cggcggttgg tcattcgtcc tgggttttct
     2821 acgctgatcg acgaaaacag cgagttcggt acgtacaatg gacgattatc aaaggtcgta
     2881 attttggtaa ttcgccagcc aatttccgct actgtacctt cgatatttct gtccggtgaa
     2941 cggatccagt cgccaatact gaaaggacgg tcgaaataga gcatgatccc ggaaaagaaa
     3001 ttactcagaa tatctttacc ggccataccg acagccagac caccaatacc accaaaggtc
     3061 agcaagccag aaaggctcat gccgaaatgt tcgccataaa gcagaacaag cactacaata
     3121 atggtgattt tgataatgcg cgacataatc cgcgcactgg tgatatcgcg acctttttta
     3181 atctgctgtt tttcaaactg attaatcagc agaaatagct taatcgtcag aataaccgca
     3241 atcagggacg tacagataaa atcgataacg cctggggtga taaatttgag tttatagttt
     3301 tctataacat aattactgat gctaccaaca gcactgataa ttatggtgta gattaaaaat
     3361 tgcaccgcat ggaataaaaa tccttttctt ttacgatttc cacggcgaaa ccaaaagctc
     3421 atcagaatca atgctgcgca gctaccgaaa ataatgacca gattaagcgc attatttgta
     3481 aacagttcag cgatcattgt tttatcaggc tcctccagat aattgtcgtc atgccggaaa
     3541 cccctggc
//
LOCUS       DAEBXN010000242         3465 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.245, whole genome shotgun sequence.
ACCESSION   DAEBXN010000242 DAEBXN010000000
VERSION     DAEBXN010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3465
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.245"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(1..213)
                     /locus_tag="KJL99_004335"
                     /pseudo
     CDS             complement(1..213)
                     /locus_tag="KJL99_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            488..792
                     /locus_tag="KJL99_004336"
                     /pseudo
     CDS             488..792
                     /locus_tag="KJL99_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004952983.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-type pyocin domain-containing protein"
     gene            961..2325
                     /locus_tag="KJL99_004337"
     CDS             961..2325
                     /locus_tag="KJL99_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD4438817.1"
                     /translation="MSLFTEPKEIERLPSEEIERLYPVLRYRVFISIFLGYMGYYFVR
                     NTTSVLSGVLHMSATEIGIISCAGFLSYGISKFVSGLISDRSNSKVFLSLGLFLSGLV
                     NFLIGYIPGIITSVTLFSTMYLLNGWIQGMGYPPGAKTLVFWYEHRERITWATLWNLS
                     HNVGGALAPVLIGFSFGFFGDSALDHARAAFIFPGVLCMAMSVLIYFIQVDRPVSVGL
                     PPIEEWKGNVVSHPAKGREQGPRLSIPDIIRKHIIRNNKLIYCCIYGSFVYILRYGIV
                     SWAPKFLSDSLDVGGKDMGKLASMGGGSVFEIGGVAGMLLAGYLSVRLFRNSKPLTNT
                     LFLALTIILLIAYWYVPSGNEYLWLNYTILILLGLAVYGPVMFIGLYSMELVPKEAAG
                     AASGLSGTFSYIFGSIVATLGMGLVVDYLGWGATFIVLILSAVFAIIFTLMSRERSLE
                     FEKE"
     gene            2375..2584
                     /locus_tag="KJL99_004338"
                     /pseudo
     CDS             2375..2584
                     /locus_tag="KJL99_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683482.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
     gene            complement(2678..2869)
                     /locus_tag="KJL99_004339"
                     /pseudo
     CDS             complement(2678..2869)
                     /locus_tag="KJL99_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(2910..3407)
                     /locus_tag="KJL99_004340"
                     /pseudo
     CDS             complement(2910..3407)
                     /locus_tag="KJL99_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          846 a          782 c          823 g         1014 t
ORIGIN      
        1 tgaacctcga tcggtgtggt ggattagccc ggcaggtggg cgctggctcc tgagcgccat
       61 aaacagggct ttacccgtca actctttcgt catgcgctct cccatggcgt aaccgacaat
      121 ttcgcacgta taaacatctt tgacgccagc gagatacagc catccttccc gggtggcaac
      181 atacgttata tctgcgaccc agacctgatt aggggggttg gggagcaatg gaacagtaaa
      241 cgccgttaag aagtcaattt aaatagccaa aaattaacta gccaatttac attgcaggtt
      301 gtaattttta accacctttt ttacgatagc caaattacct atacccatcc cggccctgcc
      361 aagccatacc cgctatcggc gatgaaaatg cttttgtgcg cggtcggagt ggtcccgacg
      421 agggttttac ccgaagtcgg ggcgtatctc cgcgttagcg ggccgtgagg gccgcttacg
      481 agcgtgtatg agagacgtac agtggttttg tccgctcaca ccagtacata caggtacgga
      541 aataaaacca gtcgaaatgc cggtaacgac gattactcct gtctctgatg ccggtggact
      601 acaagatttt atttactggc gtcctgatgc ggacagggct ggtatggaac ctgtttatgt
      661 gatacttaat gatcctctgg attccgggcg atttacgcgt aaacaactaa ataaaaatat
      721 cttaaacatg caagtaattt tggtattctc gatacaagaa taaatagtag taactactat
      781 agctgcaaat aatgttaaaa acatttctat gatcatcttt acatttttca tgattgacaa
      841 ttgtttttgg ttaaaaaaag acaactaatg agtgtgataa tgcaatactt tgagtgatta
      901 tataatgata agtaaacagg tttgcgacaa ttggttatca gtattacaga aggattaaat
      961 atgtcattat tcacagaacc taaggaaatt gaaagattac cctctgaaga gattgaacga
     1021 ttatatcccg tcctgcgata cagggtcttt atcagcattt tcctcggata tatggggtat
     1081 tattttgtcc gaaacaccac cagtgtgtta tccggtgtgc tgcacatgtc tgcaaccgag
     1141 atcggcatta tctcctgtgc cggtttttta tcctatggta tatcaaagtt tgtttccgga
     1201 cttatttctg acagaagtaa ttcaaaagta tttttgtctc ttggtttatt cctgtccgga
     1261 ctggtaaatt ttctgatagg atatattccc gggatcatca catccgttac gttattttca
     1321 acaatgtatt tacttaacgg ctggatccaa ggtatgggat atcctcctgg tgccaaaaca
     1381 ctggtgttct ggtatgaaca tcgggaaagg atcacctggg ccacactgtg gaatttatcg
     1441 cacaatgtcg ggggagctct ggcccccgtt ctgattggtt tcagttttgg ttttttcggc
     1501 gacagtgctc tggatcatgc ccgggccgca tttatcttcc caggagtact gtgtatggca
     1561 atgagtgtgc tgatttactt tattcaggtg gacagacctg tatcagtggg gcttcctccc
     1621 attgaagaat ggaaaggtaa cgtcgtttca cacccggcta aaggccgtga acaaggtccg
     1681 agactctcca ttcctgatat catcagaaaa catatcatcc gaaataataa actcatttac
     1741 tgctgtatct atggttcgtt tgtttatatt ctgagatacg gtattgtttc ctgggctcct
     1801 aagtttttaa gtgacagtct ggatgtcggt ggtaaggata tgggaaaact ggcatcaatg
     1861 ggggggggtt ctgtttttga aatcggtggt gtggctggta tgcttcttgc cggatacctg
     1921 tctgtcagac tgttcagaaa ctcaaaacca ttaacaaata ctttatttct ggcacttacc
     1981 attattttat taatcgcgta ctggtacgtg ccatcaggca atgaatatct ctggctaaac
     2041 tatacaatcc ttattctgtt ggggctggca gtgtatggtc cggtgatgtt tattggtctt
     2101 tattcaatgg agcttgttcc taaagaagct gccggagccg catcagggct gtcaggcaca
     2161 ttttcctata tcttcggctc aattgttgcg acactgggaa tgggacttgt tgtcgattat
     2221 ctgggatggg gggccacatt tatcgtactg atactgtctg ctgtttttgc catcattttc
     2281 acactgatga gtcgtgaacg ctcactggag ttcgaaaaag agtaattttc tcaggaaaaa
     2341 cataaaatta ctgactaaat atttattttt tgaatcgcag ggcattccgg ataaggttga
     2401 tatcgtgtca cggctgaaga cctatggata cagtatttct ggtgtggaga cggatgacgg
     2461 atataaggcg cttgtccgcg cttttcagct tcatttccgg cagaaaaatt atgacggcat
     2521 aatggatgct gaaaccgctg ccatcctgta tgcattgctg gagaagtatt tcccgggtaa
     2581 ataaggagtg agtcgtcaga gctgcgatat atctccttcc tccatgggct gtctcttata
     2641 cacaaatccc cacccgggga tttgtgatgt cagatgtccc cagtaagact atgttttcat
     2701 tgcgctctat gaagctgagt gagcgtaacg actggagctg cttctgcggt gctccggtgg
     2761 cgaacgtgaa gtcatactct tcgaacattt tcaccgccgg gaaggctgcc attcgggtat
     2821 acatcgcctg tttacgttga tgacgtgcca gtttttcttc atgaagcatg atgctccaag
     2881 taaacgttca ccgaacaccg tattgacgat tatcctgatg gtagcaccac cttccatggc
     2941 aggaggtcgt caactcggtt ggaaggccat tccggcagta cgctcagaat atggcgcaga
     3001 tacgcttccg gatcgatacc gttcagacgg caggtgccga tcagcccgta cagcagtgca
     3061 ccacgctcgc cgccgtgatc gctaccgaag aacacgtaat ttttctttcc gagacagact
     3121 gcacgaagcg ctctttccgc tgtgttattg tccgcctccg ccagaccgtc atcactgtaa
     3181 taacagaggg cgtcccactg attcagtaca tagctgaatg cttcgcccag tctggatttt
     3241 ttcgacagcg tgccattctt ctccaccatc cattcatgca gcgacgtcag taacgctttg
     3301 cttcgctgct gcctgactgc aagacactct gactctggta atccccgtat ttcatcctcg
     3361 atggcgtaca gttcactgat tcgcttcagg gcttcttctg ccgtcgcacg caagtaatcc
     3421 ggggcccgct atgccgcgct ctatcgggcg ggacggggct ggcgc
//
LOCUS       DAEBXN010000243         3414 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.246, whole genome shotgun sequence.
ACCESSION   DAEBXN010000243 DAEBXN010000000
VERSION     DAEBXN010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3414
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.246"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..238
                     /locus_tag="KJL99_004341"
     CDS             <1..238
                     /locus_tag="KJL99_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HBD4438818.1"
                     /translation="LLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMP
                     ALSEKIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            248..886
                     /locus_tag="KJL99_004342"
     CDS             248..886
                     /locus_tag="KJL99_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HBD4438819.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCSDSSDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRFGSGKKESYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            889..2052
                     /locus_tag="KJL99_004343"
     CDS             889..2052
                     /locus_tag="KJL99_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HBD4438820.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSRELFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            2136..>3414
                     /locus_tag="KJL99_004344"
     CDS             2136..>3414
                     /locus_tag="KJL99_004344"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HBD4438821.1"
                     /translation="MHWQTHTIFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPVTMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEG"
BASE COUNT          760 a          803 c          987 g          864 t
ORIGIN      
        1 attgttaggt tacgttatcc cattatccag tgcggcaatc aatgcggtga gtattccaga
       61 ctatttcgcc tggggcggga ttgcactggt gattcagtta ctcgtttttg ctggtgtcag
      121 actttatatg cccgcattaa gcgaaaaaat tattaatcac aataccgcag caggaatgtt
      181 tatgggaacc gccgcgctgg ctggcggtat ttttaacgca gcttgtatga catggtaatg
      241 gacgatcatg gccagaaaac gcaaatcaag aaataacagt aaaattggtc acggagcgat
      301 cagtcgcatt ggtagaccga ataatccttt tgaaccgcgt cgcaatcgct acgcacaaaa
      361 atacttaacg ttggcgctaa tgggcggtgc cgcttttttc gtattgaaag gttgtagcga
      421 tagtagcgat gtcgataacg acggcgacgg aacattttac gcgacagtgc aggattgtat
      481 tgatgacggt aataatgccg acatttgcgc gcgtggctgg aacaacgcca aagcggcatt
      541 ttatgccgat gttccaaaga atatgactca gcagaactgt cagtctaagt acgaaaattg
      601 ctactacgac aatgttgaac agagttggat cccggtcgtt tctggatttt tgttaagccg
      661 ggttattcgt aaagatcgcg atgagccgtt cgtctataac agcggcggat cctcatttgc
      721 ttcccgcccc gtctggcgca acacttccgg tgattactcc tggcgcttcg gttctggcaa
      781 aaaagagtct tactcttcag gcggctttac caccagaaaa gcgtcgaccg tttcgcgcgg
      841 cggctatggt cgttcttcca gcgcccgtgg gcattgggga ggctaatcat gctgagacac
      901 aacgttcctg tgcggcggga tctggaccag atcgccgcta acaacggttt cgactttcat
      961 atcatcgaca atgaaatcta ttgggacgag agtcgggctt accgttttac tctgcgccag
     1021 attgaagagc agatcgaaaa accgactgcg gaactgcatc agatgtgcct tgaggtggtg
     1081 gatcgcgcgg ttaaagacga agagatcctg acgcaactgg cgatcccgcc gttgtactgg
     1141 gatgtgatcg ccgaaagctg gcgcgcacgc gatccttcgc tgtatggccg gatggatttt
     1201 gcctggtgtg gcaatgcgcc ggtgaagctg ctggagtaca acgccgatac gccgacctcg
     1261 ttgtatgagt cggcttattt ccagtggttg tggctggagg atgcccggcg cagcggcatt
     1321 attccgcgtg atgccgatca gtacaacgcc attcaggaac gcctgatttc gcgctttagt
     1381 gagctttaca gtcgggaact gttttatttt tgctgctgtc aggacaccga tgaagacagg
     1441 agtaccgtgc tgtacttgca ggactgcgcc cagcaggcag ggcaggagtc gcggtttatc
     1501 tacattgaag atctcggttt gggcgtcggc ggcgtactga ccgatcttga tgataatgtc
     1561 atccagcgtg catttaagct gtatccgctg gagtggatga tgcgtgacga taacggtccg
     1621 ctgctgcgca agcgtcgcga gcaatgggtg gagccgttat ggaaaagtat cttgagtaat
     1681 aaagggctaa tgccgctgct ttggcgcttc ttccctggtc atcctaacct tcttgcatcc
     1741 tggtttgagg gtgaaaaatc gcagattgcc gctggcgaaa gctatgtgcg taaaccgatc
     1801 tactcgcgcg aaggcggaaa cgtcaccatt ttcgacggtc agaataacgt cgttgaccac
     1861 gctgatggtg attacgccga tgaaccgatg atctaccagg cgtttcaacc tctgccgcga
     1921 tttggcgata gctacacact catcggtagc tggattgtcg atgatgaagc gtgcggaatg
     1981 gggatccgtg aagataacac actgatcacc aaagacacct cacgtttcgt tccgcattac
     2041 attgctggat aagaatgttt tagcaatctc tttctgtcat gaatccatgg cagtgaccat
     2101 actaatggtg actgccattg atggagggag acacagtgca ctggcaaact cacaccattt
     2161 ttaatcaacc tataccatta aataacagca acttatacct gtctgatggc gcgctttgcg
     2221 aagcggtaac gcgtgaaggt gctggctggg atagcgattt tctagccagt attggtcagc
     2281 agttaggaac ggctgaatcc cttgaactgg ggcggctggc gaatgtaaac ccgcctgaac
     2341 tgttgcgcta cgatgcccag gggcggcgtc tggacgatgt tcgtttccat cccgcatggc
     2401 acctgctgat gcaggcgtta tgtaccaatc gggtgcacaa tcttgcctgg gaagaagacg
     2461 ctcgctccgg cgcatttgtg gcgcgcgcgg cgcgttttat gttacatgcg caggttgagg
     2521 ctgggtcgtt atgtccagta acgatgacct ttgccgccac gccattgttg ttacagatgt
     2581 tacccacgcc gtttcaggac tggaccacgc cgctgctgag cgatcgctac gattctcact
     2641 tattgccagg tgggcaaaaa cgcggtttgt tgattggcat gggaatgacg gaaaagcagg
     2701 gcggttccga tgtcatgagc aacaccaccc gtgcagagcg tttggaagat ggctcttatc
     2761 ggctggtggg gcataaatgg tttttctcgg tgccgcaaag cgatgcgcat ctggtgctgg
     2821 cgcagaccgc gggcggtctg tcctgctttt ttgtgccgcg ctttttgcct gacgggcaac
     2881 gcaacgcgat tcgcctcgag cggctgaaag ataagctggg taatcgctct aacgccagtt
     2941 gcgaagtgga gtttcaggat gccattggct ggttattggg gcaggaaggg gaaggaattc
     3001 gtctgatcct gaaaatgggg gggatgacgc gttttgattg cgccctgggt agccatgcca
     3061 tgatgcgccg tgcattttcg ctggcgattt atcatgcaca tcaacgccat gtttttggta
     3121 atccattgat ccaacagccc cttatgcgtc atgtcttaag tcgcatggca cttcagcttg
     3181 aagggcaaac ggcgttgctg tttcgtcttg cgcgagcgtg ggaccggcgt gccgatgcca
     3241 aagaagccct gtgggcgcgt ttatttacgc ctgcggcaaa atttgtgatc tgcaaacgag
     3301 gtatgccgtt tgtggccgaa gcgatggagg tgctgggcgg cattggttat tgcgaggaga
     3361 gcgagctgcc gcggctttac cgggagatgc cggtaaacag tatttgggaa ggtt
//
LOCUS       DAEBXN010000244         3348 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.247, whole genome shotgun sequence.
ACCESSION   DAEBXN010000244 DAEBXN010000000
VERSION     DAEBXN010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.247"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            19..897
                     /locus_tag="KJL99_004345"
     CDS             19..897
                     /locus_tag="KJL99_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBD4438822.1"
                     /translation="MMTFNLREQQKRLQARMDELRAEIAFAQKGEKPWPYRSCLMREG
                     RGYCEKHGEYHTHILVWSDRNGEDREEISCCPDCLIAEANDLTMELSSIKAEELTDNA
                     GIALRFRDCEFDNYLEVNPGAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVAMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADEVIERYASMDLLIIDEV
                     GVQFGSAAEMAILQEIINARYESILPTILISNLSPEELWAFISPRIADRITDGGRNWL
                     SFNWPSYRSRIRGVAA"
     gene            894..2285
                     /locus_tag="KJL99_004346"
     CDS             894..2285
                     /locus_tag="KJL99_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBD4438823.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFSVRA
                     YQDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWTRFSQGVGRMTG
                     RNIWMVDQANLAIDEICATTKHHLIKYPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQARGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESARVLSTPMKPGNPRRYS
                     NKRTDSSKMERFF"
     gene            2282..2539
                     /locus_tag="KJL99_004347"
     CDS             2282..2539
                     /locus_tag="KJL99_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438824.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEALR"
     gene            2634..2834
                     /locus_tag="KJL99_004348"
     CDS             2634..2834
                     /locus_tag="KJL99_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HBD4438825.1"
                     /translation="MSVKIQTIPELLIQTRGNMTEVSRMLNCNRATVRKYAEDKEGKG
                     HAIVDGVLIVHRGWDRGKDSDA"
     gene            2827..3207
                     /locus_tag="KJL99_004349"
     CDS             2827..3207
                     /locus_tag="KJL99_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HBD4438826.1"
                     /translation="MRDIQQVLERWGAWVANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMVICGCMARLNRNNNDLHDLLVDYYVFGMTFMTLARKHGCSDGYIGKKLQKA
                     EGVVEGMLMMLGVRLEMDKYIEHP"
BASE COUNT          885 a          726 c          969 g          768 t
ORIGIN      
        1 cattctgagg tgacagcgat gatgacgttt aacctgcgtg aacaacaaaa aagactacag
       61 gcgcgaatgg atgagttacg ggcagagatt gcatttgctc agaagggcga aaagccatgg
      121 ccttatcgtt cctgcctgat gcgtgaaggt cgcggatatt gcgaaaaaca tggcgaatat
      181 cacacgcata tactggtgtg gagcgatcgt aatggcgagg acagagaaga aatttcatgc
      241 tgccctgact gcttaatcgc tgaggccaac gatttgacca tggagctgtc gtccatcaag
      301 gcggaagagc tgactgataa cgccggaatt gccctgcgtt ttcgggactg cgagtttgat
      361 aattatctgg aggttaatcc tggcgcagcc agaaatcttg cggcctgtcg ccgctatgcg
      421 gagaactggc cagatatgct ggagaacggt accagtcttg ttatgaccgg cagttgtggt
      481 accggaaaaa atcatctggc tgtggctatg gcaaaacaca tcatccgtaa ctatctggcc
      541 agtgtggaga tcaccgacgt gatgcgcctt acccgtgctg tgaaaaattg ctggcggaat
      601 gacagcgaaa aaacagcgga tgaagttatt gagcgttatg cgtcaatgga tttgctgatc
      661 atcgacgaag ttggcgttca gtttggcagc gcggctgaaa tggctatttt gcaggaaatt
      721 atcaacgcca ggtacgaaag tatcttgccc accattctga tcagtaacct ctcaccggaa
      781 gagttgtggg cgttcatcag tccccgaatt gccgacagga tcacagacgg gggacgcaac
      841 tggttgtcgt ttaactggcc cagttaccgt tctcgtatca gaggtgtggc tgcatgacaa
      901 ctccagtatg gcgtaacgat gaccttgaag gcgctgtcat cggcgcgttc tttctgcgcg
      961 gagcagatcc tgaagtgatg gatattctgg ccacactgcc agcggacgtt ttttctgtac
     1021 gagcgtatca ggatatctat acaggcatct gcagacaggc ccgtgtttca ggagtgattg
     1081 accccgtgct gttgtgtaat gagatgccgg aacttgcccc ggtgattact gataccgggc
     1141 gtaaaacctg ggtgaagtct tcactggagc actatgttgc agcgttgcgg cgcaatgccg
     1201 cactgcgcga tgcagaaaaa acactgaatg aggcgctgca gaaattacgt gatgcgcata
     1261 cctgtgaagc agctgaagat gccctgaagg atgcgcagaa catgatggtc acactgtcga
     1321 caggaaaggg cgtcattcag ccggtacata ttgatgatgt gcttccggaa gtggttgagc
     1381 gtgttgaatg ccggaatcag gggctggaga aatccaggac gttgatgacc ggtattgatg
     1441 aactggacgc aaaaacaggc ggtatggagc ccggagacct ggtattcatt gccgcccgtc
     1501 cttcgatggg gaaaaccgaa cttgcgctgg acatcatcga caaggtgact gagcaggggc
     1561 atggtgtgct tctgttcacc atggagatgg cgaacatcca gattggtgaa cgtatggtgt
     1621 ctgcggctgg agggatgcca gtatcacgcc tgaaatctgt ggctcacttt gaagatgaag
     1681 actggacgcg tttctcacaa ggggtggggc ggatgaccgg gcgcaatatc tggatggtgg
     1741 accaggcgaa cctggccatt gatgagatat gcgcaacaac gaaacaccac ctgattaaat
     1801 atccggaaac ggcgctggtg gtggttgatt atctcgggct gataaaaacc cgaaccacgg
     1861 ggcgtcatga ccttgccgtg ggtgaaatct caaaggggct taaaggcctg gcaaaatccg
     1921 gtggttttcc gttgattgcg ctgagccagc tctcccgcgg tgtggagtcc agacccaata
     1981 aacgtcccat gaactcagac ctgaaaaatt ccggagaaat agaggcggat gcagacatca
     2041 ttctgatgct ttacagggat gaagtgtaca acccggatac gcaggccagg ggcatcgcag
     2101 aaatcaatat cacgaaacaa cgtaacggtt ctctgggaac gatttaccgg cgtttttata
     2161 acggacattt tctgcctgtg gaccaggaaa gtgcacgggt gctttccact cccatgaagc
     2221 ccggcaatcc gcgcagatac agtaataaac gaactgacag cagtaagatg gagcgtttct
     2281 tttgaacaac cagacaatga cttttacccc tgaacaattg cgtaaacacg cgcaggaaat
     2341 gctgcgtcat gctgaacaac tcgaaaaaac gggcataaca aaagatgcta tccgtaaaga
     2401 tatggtaccc gcgcttcggg aactgatgca ggcgaagcat cgcgcacaga aagcagtaga
     2461 tgagctggtg gactgtgtgg cagagctgga aaccagagtc ggaaagtttg aaaaactggt
     2521 gcaggaggcg ctgcgctgat gcgccatgag tttgttttgc cctggcttgt gcgcgatcat
     2581 tatgtccctg gtggaaagcc cgggataaaa atcaccgaac cggagagtat ttgatgagcg
     2641 tcaaaattca aacgataccg gaactgctta tccagacacg aggtaatatg accgaagttt
     2701 cacgaatgct gaactgtaat cgcgccacgg taagaaaata tgctgaagat aaagagggca
     2761 aagggcacgc cattgttgat ggtgttctga ttgttcatcg tggatgggac aggggtaaag
     2821 acagtgatgc gtgatattca gcaggttctt gagcgctggg gcgcgtgggt ggctaataat
     2881 catgaggatg tgacctggtc gtccattgct gccggtttta agggattaat cccttcaaaa
     2941 gtaaaatccc gcccgcaatg ctgtgacgat gacgcgatgg ttatatgtgg gtgtatggcc
     3001 cgccttaacc ggaacaacaa cgatctacat gacttgctgg ttgactacta cgtgtttggg
     3061 atgacattca tgacgctggc gcggaagcat ggatgctcag atggttatat aggaaagaag
     3121 ctacaaaaag ccgagggggt ggtggaagga atgttgatga tgttgggtgt ccggttagaa
     3181 atggacaaat atattgaaca tccctgatgg taaggggcga ctttctagaa aatattttac
     3241 gtacgtaaaa aacgggatat tctgttaaga gtggttgctt cgccacacag cttaaacccg
     3301 cctccgggcg ggttttttat gcctgaaaaa cggtacagga cgttaaac
//
LOCUS       DAEBXN010000245         3325 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.248, whole genome shotgun sequence.
ACCESSION   DAEBXN010000245 DAEBXN010000000
VERSION     DAEBXN010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.248"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            454..1281
                     /locus_tag="KJL99_004350"
     CDS             454..1281
                     /locus_tag="KJL99_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HBD4438827.1"
                     /translation="MKKVIPALALVLLTTGLVGCDRVEPGNVGIKVNKLGDDKGVGEV
                     VGVGRYWTGWNTEVYIFPTFKQMKTYDEPFSFQMSDGTTIGYHIGVAYKVDPSKVTTV
                     FQTYRKGVDDITDTDLRQKIADALNRLASKMTTDKFIDGGKSELLDAALKDIQEEMTP
                     IGIQVMSLSYVGKPEYPPTVIDSINAKVTANQKTLQCEQEVKQREAEANMLRAEAAGQ
                     ADAIRTKAQAEADAIRLRGEALRQNPNVMELEAINKWNGTLPQYMTSGANTPFIQVK"
     gene            1325..2074
                     /locus_tag="KJL99_004351"
     CDS             1325..2074
                     /locus_tag="KJL99_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HBD4438828.1"
                     /translation="MNTVTINNKQFPVIEYRGQRVVTLAMIDEVHQRPEGTARAAFNR
                     NREHFISGVDYAELGADVIRTDLPEGTFSKFAPSGIVLFESGYLMLTKPFNDALAWQV
                     QRELVNSYFRTRTPLTEIEMIAAIAADAVRQQKRLNHVEEQLETVTEAVETIKRGNMR
                     AGYVGYRQVVAKSGMSDTKCRNLVNAYQIPTDTHEFMTPDGLLSRRAIVELEPFMAAF
                     HQMMSEADPRGTRWYHPKMGLFQVIGWEDKA"
     gene            2071..2640
                     /locus_tag="KJL99_004352"
     CDS             2071..2640
                     /locus_tag="KJL99_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438829.1"
                     /translation="MIIQSKLIRAALVCAAKNDVRYYLNGLHITPKHIEATNGSVALR
                     MAHGIRTKKNIIVQFEGGVPAKAETTELIFSKEPIAVHRDQFQRRLSITGIKLVDGCF
                     PDLDRIIPKKFDRCTHPVLQAGYLSYPEKMFGRERKFIPVQLRPSGDGQAVRIQFDSI
                     INSMYGNPEFVVMPCRDHGDFNVAQEHPE"
     gene            2764..3006
                     /locus_tag="KJL99_004353"
     CDS             2764..3006
                     /locus_tag="KJL99_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HBD4438830.1"
                     /translation="MFALIQRGQIYTDRAGYPVEITRSTEHSVFFRRMDGRTGRVRIG
                     EFSSLFEHIDHLEYHKILAETEQEKHLKKLRAMQRK"
     gene            3010..3156
                     /locus_tag="KJL99_004354"
     CDS             3010..3156
                     /locus_tag="KJL99_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438831.1"
                     /translation="MNKAFELWVHQRYGNHYDLTRDVDGFYCREIVKRMFEVWCHCRG
                     LSVV"
     gene            3165..>3325
                     /gene="xisR"
                     /locus_tag="KJL99_004355"
     CDS             3165..>3325
                     /gene="xisR"
                     /locus_tag="KJL99_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HBD4438832.1"
                     /translation="MGNVIQLAPNEWVCESVLIAITGLKPGTILRTRKECWMVGREYI
                     HVSPDGNPKP"
BASE COUNT          928 a          796 c          855 g          746 t
ORIGIN      
        1 acgccatcct gacaagcgtt ttatcttcaa cgggaatgag atatggaaac gtggagaatc
       61 aaccacagag tatacccgcg cacaccagcg aacaatcaga cgactgcgca gactcatcgc
      121 caggaaacag tatacagaaa aatggttcag aaaatatgac acatggagcg ccggatatta
      181 cgaactgatg gcaacaaaac cattctgacg taaacgaaat taaccatgac gcaattaaat
      241 aaggcaagcc gaatacatca ggaggaccat gaacatttat ttcagaatag ttatatcact
      301 ggcaattatc gcatgtattt acggattaat cgttccggcc ctcatatcaa tgaaggatac
      361 ggtagcagtg atttctggct ttgctctggc gtgtctgacc ccgccctgca tttatgccat
      421 ttataagggt ctttcttttt ctaaggataa aagatgaaaa aagttattcc tgctttagcc
      481 cttgttctgc tgaccaccgg acttgtcggt tgcgatcgcg ttgagcctgg taatgttggc
      541 atcaaggtaa acaaactggg cgacgataaa ggcgtcggag aagtggtcgg tgttgggcgc
      601 tactggacgg gatggaacac tgaggtttac atcttcccga ccttcaaaca aatgaagacc
      661 tacgatgagc cattcagctt ccagatgagt gacggtacaa ccatcggcta tcacatcggc
      721 gtggcctaca aagttgatcc atccaaagtt accacagtct ttcagaccta ccgcaaaggc
      781 gtggacgaca ttaccgacac cgacctgcgc cagaagatag ccgacgcact caaccgactg
      841 gccagcaaaa tgaccaccga caaatttatc gacggcggca agtctgagct gctggatgca
      901 gctcttaaag acattcagga agaaatgacg cccatcggta ttcaggtaat gagcctctca
      961 tatgtgggta agccggagta cccgcctact gttatcgaca gcattaatgc caaagtcacg
     1021 gcgaaccaga aaaccctgca atgcgagcag gaagtaaaac agcgcgaagc ggaagccaac
     1081 atgttgcgcg cggaagctgc cggacaggca gatgcgattc gcacaaaagc ccaggccgaa
     1141 gccgatgcta ttcgtttacg cggtgaagct ctgcgccaga acccaaacgt tatggagctg
     1201 gaagccatca acaagtggaa cggtacactg ccgcaataca tgaccagcgg tgccaacaca
     1261 ccatttattc aggttaagta atacatatgc ccggtattac acgccggggt gtctggagat
     1321 aaaaatgaat actgtaacca tcaacaacaa acagtttccg gtaatcgaat atcgcggtca
     1381 gcgtgttgtg acgctggcga tgattgatga agtccaccag cgaccggaag gaaccgcgcg
     1441 tgcagcattc aaccgaaacc gtgagcattt catcagcggt gtggattatg ccgaattagg
     1501 tgcggacgta atacgtacgg acctcccgga aggaacattc tctaaatttg caccatcggg
     1561 gattgtactt tttgaatcag gttacctgat gttgacgaag ccattcaatg acgcccttgc
     1621 ctggcaggtt cagcgcgaac tggttaacag ctacttccgc actcgcacac cgctaacgga
     1681 aatcgagatg atcgctgcga tagccgccga cgctgttcgt caacagaagc gcctgaatca
     1741 tgtggaagaa cagctcgaaa cagtcacaga agcggtggaa accatcaaac gcggaaatat
     1801 gcgcgccgga tatgtcggtt accgccaggt ggtagccaaa agtggaatga gtgacaccaa
     1861 gtgtcggaat ctggtgaacg catatcaaat tcctacagac acccacgaat tcatgactcc
     1921 ggacggtctg ttgtctcgca gggctatcgt cgagcttgag ccatttatgg cggcgttcca
     1981 ccaaatgatg tcagaggctg acccacgcgg cacacgctgg tatcacccga agatggggct
     2041 tttccaggtg atcggatggg aggataaagc atgatcatcc agtcaaaact tattcgcgcc
     2101 gctctggtgt gcgctgctaa aaacgacgtt cgttattacc tgaacggtct tcacatcacg
     2161 ccgaaacata ttgaggcaac caatggttcc gtggcactgc gcatggctca cggcatccgg
     2221 acgaagaaaa acatcattgt ccagttcgaa gggggcgtcc cggccaaagc cgaaacgaca
     2281 gagctgattt ttagtaaaga gccgatcgct gttcatcgcg accagtttca gcgacgactg
     2341 tccattaccg gcattaaact ggtggacggt tgttttccgg atttggatcg catcattccg
     2401 aaaaagtttg accgctgtac tcacccggtg ctccaggcgg gttacctgag ttatccagag
     2461 aagatgtttg gtcgtgagcg taaatttatt cccgtccagt tacgtccctc cggtgacggg
     2521 caagcggtca gaattcagtt tgattccatc atcaactcaa tgtatggcaa tcctgaattt
     2581 gttgtgatgc cttgtcgtga tcatggcgat ttcaacgtgg ctcaggagca tccggaatga
     2641 aaatcgaata ccaggacgcc gcaggaggtg aatcaatgag ctggcctgat gcaatcgtaa
     2701 ctctggggat ggtattcgca gtagcgtttg ttgtgtactc gatttgtcga tggggataac
     2761 cacatgttcg ctttgattca acgcgggcag atatacacag acagagccgg atacccagta
     2821 gaaattactc gcagtactga gcactcagtg ttctttcgga ggatggacgg acgtaccggg
     2881 cgagtacgca tcggtgaatt cagtagcctg ttcgagcata ttgaccacct ggaatatcac
     2941 aagattctgg ctgaaacaga acaagaaaag cacctgaaga aattacgcgc aatgcaaagg
     3001 aagtaaaaaa tgaataaagc gtttgagcta tgggtccacc agcgttacgg caaccactac
     3061 gacctgacgc gagatgttga cggtttctac tgtcgtgaaa ttgtgaaacg aatgtttgag
     3121 gtctggtgcc actgccgtgg gctgagtgtt gtgtgaggta atgcatgggc aatgtgattc
     3181 aactggctcc caatgaatgg gtttgtgaaa gcgttctaat cgcaattacc gggctcaaac
     3241 caggcacaat tcttcggacc cggaaagaat gctggatggt tggaagagag tatattcacg
     3301 tatcaccaga cggtaatcca aagcc
//
LOCUS       DAEBXN010000246         3277 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.249, whole genome shotgun sequence.
ACCESSION   DAEBXN010000246 DAEBXN010000000
VERSION     DAEBXN010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.249"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..66
                     /locus_tag="KJL99_004356"
     CDS             <1..66
                     /locus_tag="KJL99_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="with Hns involved in transcriptional regulation of
                     hemolysin; non-specific DNA-binding protein which affects
                     the production of multiple proteins; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBD4438833.1"
                     /translation="AELITGKLYDRIPKEIWKYVR"
     gene            105..695
                     /locus_tag="KJL99_004357"
     CDS             105..695
                     /locus_tag="KJL99_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HBD4438834.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVELRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            935..1195
                     /gene="repA"
                     /locus_tag="KJL99_004358"
     CDS             935..1195
                     /gene="repA"
                     /locus_tag="KJL99_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HBD4438835.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            1419..1493
                     /gene="tap"
                     /locus_tag="KJL99_004359"
     CDS             1419..1493
                     /gene="tap"
                     /locus_tag="KJL99_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HBD4438836.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            1486..2343
                     /gene="repA"
                     /locus_tag="KJL99_004360"
     CDS             1486..2343
                     /gene="repA"
                     /locus_tag="KJL99_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HBD4438837.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            3044..3235
                     /locus_tag="KJL99_004361"
     CDS             3044..3235
                     /locus_tag="KJL99_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438838.1"
                     /translation="MAENGYGLAGLGMGKVKSVNQYRLTPGFGGFTPVSHVTTACRLP
                     CRWRGIRIIQAAFNAFAKV"
BASE COUNT          870 a          811 c          832 g          764 t
ORIGIN      
        1 gcggaattaa tcaccggaaa gttatatgac aggataccaa aagagatatg gaaatatgta
       61 aggtaataat tcagcaataa ttaattgcca ggagcagata aataatgaag ttgattattt
      121 ttattctcat tgttctgatt attgcagcgc tacttatccg gattatcctc agaagtgtaa
      181 atcagcattc tcctttgctg atgcaattgc atgcggcagg tatacggact ggtgatgcag
      241 agcgaatact gtccagcggt gaatactggc aacgtcagaa gacgctgctg acagaaaggg
      301 aagtcagttt tatgaaagga ctgttcagaa ttgtggatat gaagcggtgg tatctgtgtc
      361 cgcaggtacg ggtagcggat atcgtccagc tgaacgggaa tatccggcca cgatcacgcc
      421 agtggtggca gttattcagg atggtgtctc agtggcatgt tgatgtggtt atcgttgagc
      481 tgcgttcgtt cagtattgtt gcagcagtag agctggatga tgccagccat ttacgaccgg
      541 aacgcagacg ccgggatatt cttctggaag aggttctgag gcaggctggt attccgttgc
      601 tcagaagcca cgatgccaga aaactgctgc agatgaccgg agaatggctg aatacaacag
      661 gggctgatca gcagtcaccg gaacatcgta gctgacgcct tcgcgttgct cagttgtcca
      721 accccggaaa cgggaaaaag caagttttcc tcgctcccgg cgtttccata actgaaaacc
      781 atactatttc acagtttaaa tcacattaaa cgacagtaat ccccgttgat ttgtgcgcca
      841 acacagatct tcgccacaat tctcaagtcg ctgatttcaa aaaactgtag tatcctctgc
      901 gaaacgatcc ctgtttgagt attgaggagg cgagatgtcg cagacagaaa atgcagtgac
      961 ttcctcattg agtcaaaagc ggtttgtgcg cagaggtaag cctatgactg actctgagaa
     1021 acaaatggcc gctgttgcaa gaaaacgtct tacacacaaa gagataaaag tttttgtcaa
     1081 aaatcctctg aaagatctca tggttgagta ctgcgagaga gaggggataa cacaggctca
     1141 gttcgttgag aaaatcatca aagatgaact gcagagactg gatatactaa agtaaagact
     1201 ttactttgtg gcgtagcatg ctagattact gatcgtttaa ggaattttat ggctggccac
     1261 gccataaggt ggcagggaac tggttctgat gtggatgtac aggagccaga aaagcaaaaa
     1321 ccccgataat cttctttaac tttggcgagt cagaaagatt accggggccc acttaaaccg
     1381 tatagccaac aatcaagcta tgcggggagt atagttatat gcccggaaaa gttcaagact
     1441 tctttctgtg ctcgctcctt ctgcgcattg taagtgcagg atggtgtgac tgatcttcaa
     1501 caaacttatt accgccaggt aaagaacccg aatccggtgt tcacaccccg tgaaggtgcc
     1561 cggacgcttc cgttctgcgg gaaactgatg gaaaaggcgg tgggcttcac ctcccgtttt
     1621 gatttcgcca ttcatgtggc acatgcccgt tcgcttggtc tgcgtcggcg catgccaccg
     1681 gtactacgtc gccgggccat tgatgcgctg ctgcaggggc tgtgttttca ctatgaccca
     1741 ctggccaacc gcgtccagtg ttccatcacc acgctggcca ttgagtgcgg actggcgacg
     1801 gagtctgctg ccggaaaact ctccatcacc cgggccaccc gggccctgac gttccttgca
     1861 gagctgggac tgattaccta ccagacggaa tatgacccgc ttatcgggtg ctacattccg
     1921 accgatatca cgttcacatc tgcactgttt gctgccctcg atgtatcaga ggaggcagtg
     1981 gccgccgcgc gccgcagtcg tgtggaatgg gaaaacagac agcgcaaaaa gcaggggctg
     2041 gataccctgg gtatggatga actgatggcg aaagcctggc gttttgttcg tgagcgtttc
     2101 cgcagttacc agacagagct taagtcccgt ggaatgaagc gtgcccgtgc gcgtcgtgat
     2161 gcggacaggc agcgtcagga tattgtcacc ctggtgaaac ggcaactgac gcgtgaaatc
     2221 tcggaagggc gcttcactgc cagtcgtgag gcggtaaaac gtgaagtgga gcgtcgtgtg
     2281 aaggagcgca tgattctgtc acgtaaccgt aattacagcc ggctggccac agcttccccc
     2341 tgaaagtgac ctcctcagaa taatccggca cgcgccggag gcattcgcac gcctgaagcc
     2401 cgccagtgca caaaaaatca gcgctacata caaaaaacaa cctcatcatc caccttcagg
     2461 tgcatccggt cccccctgtt tttgatacaa aacacgcctc acagacgggg aattttgctt
     2521 atccacatta aactgcaagg gacttcacca taaggttaca accgttcacg tcataaagcg
     2581 ccagacgtca accctacagg gtgcaatgta tcttttaaac acctgtttat atctccttta
     2641 aactacttaa ttacattcat ttaaaaagaa aacctgttca ctgcctgtcc tgtggacaga
     2701 caaatatgca cctcccaccg caagcggcgg gcccccaacc gaagccggtt tagttacaac
     2761 atgcaaacat aaacccctaa aaaacccaag tccaacgcaa aactgaaacc acaacgcccc
     2821 cctcataact gaaaagcgcc gccgcccccg cccaaagggc gggaacagag tcgcttttaa
     2881 ttatgaatgt tgtaactaca tcatcatcgc tgtcagtctt ctggctggac gtatcgaata
     2941 cacgctcgta agcggccctc acggcccgct aacgcggaga tacgccccga cttcgggtaa
     3001 aaccctcgtc gggaccactc cgaccgcgca cagaagctct atcatggctg aaaacgggta
     3061 tggcttggca gggctgggga tgggtaaggt gaaatctgtc aatcagtacc ggctgacgcc
     3121 gggcttcggc ggttttactc cggtatcaca tgtaaccaca gcgtgccgcc ttccatgccg
     3181 ctggcgcggc atcagaataa tacaagcagc atttaatgca tttgcaaaag tgtgagtaat
     3241 attaagtggt cgtttcaggg atatcgttaa gacttga
//
LOCUS       DAEBXN010000247         3220 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.250, whole genome shotgun sequence.
ACCESSION   DAEBXN010000247 DAEBXN010000000
VERSION     DAEBXN010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.250"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            143..1243
                     /gene="ompC"
                     /locus_tag="KJL99_004362"
     CDS             143..1243
                     /gene="ompC"
                     /locus_tag="KJL99_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HBD4438839.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNKRTDNSHLYGADDTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYDETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYADQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            1407..1892
                     /locus_tag="KJL99_004363"
     CDS             1407..1892
                     /locus_tag="KJL99_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HBD4438840.1"
                     /translation="MIMTNINTACVKNNASYQLNNALPNKETISSNFCERLEQWGNKS
                     VNNGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDLA
                     DNASLVNINLSVNKINTITFPKEPKLENIYINHNNLESLDLKNQHSLVNLEAQNNSLK
                     K"
     gene            2002..2991
                     /locus_tag="KJL99_004364"
     CDS             2002..2991
                     /locus_tag="KJL99_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HBD4438841.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTDIVAGNKNELL
                     LLDLSHNKLTSLHNVLFPNLNTLLINNNLLSEIKIFYSNFRNVQTLNAANNQLKYINL
                     DFLTYLPSIKSLRLDNNKITHIDTNNTSDIGTLFPIIKQSKNLNFLNVSGKNN"
BASE COUNT         1080 a          636 c          550 g          954 t
ORIGIN      
        1 atatcgataa ataatattat tctatttttc tcattgtttt taatcaccat tctattttaa
       61 aaacagtatg tcatttataa atgacgccgt ttaaaattcg tttagaaaat acgtttaacg
      121 ataattaaaa ggattctgtt aaatgaaatt aaaaatagtt gcggtggttg taactggttt
      181 gttagctgcg aacgtagcac acgctgccga agtctataac aaggatggta ataaactcga
      241 cctttatggc aaggttaccg ctctacgtta ttttactgat gataagcgtg acgatggtga
      301 taaaacttat gcccgtctcg gctttaaagg agaaacgcaa atcaatgatc aaatgattgg
      361 ttttggtcac tgggaatatg attttaaagg ctataacgat gaagccaacg gctcgcgcgg
      421 caacaagacc cgtctggcct atgctggttt aaaaattagt gaatttggct ctctggacta
      481 tggtcgtaac tacggtgtcg gctacgacat tggttcatgg accgatatgt tgccagaatt
      541 tggtggcgat acctggagtc agaaagatgt cttcatgaca taccgtacca ccggtctagc
      601 aacctatcgc aactacgatt tctttggctt aattgaaggg ctgaactttg ccgcgcaata
      661 tcaaggcaaa aataaacgta ctgacaacag tcatctttat ggtgctgacg acacgcgtgc
      721 caacggtgac ggtttcggta tctcctcaac ttatgtttat gatggctttg gtatcggtgc
      781 ggtatatacc aaatccgatc ggacaaatgc gcaggaaaga gccgcagcta atcctctcaa
      841 tgcctccggt aagaatgcag aactatgggc cacaggtata aagtatgatg ccaacaacat
      901 ctactttgca gctaattacg atgaaacatt aaacatgacc acctatgggg atggttatat
      961 ctctaacaaa gcacaaagtt ttgaagtggt ggcgcaatat caattcgact tcggcttgcg
     1021 cccatcactc gcttacctga aatcgaaagg cagagatctg ggccgctacg ccgaccagga
     1081 catgattgag tatatcgacg ttggtgcgac gtatttcttc aacaaaaata tgtcgaccta
     1141 tgttgattat aaaatcaacc tgattgatga aagcgacttt acccgtgccg tagatattcg
     1201 caccgataac atcgtcgcta cgggcattac ctatcagttc taacgtcctc tcctgaccag
     1261 cccttctctg ggctggtttt tgatttaagt gctgctgtat gactccttaa gtaaaaatga
     1321 gcaattcttg atatttctgg tcattcacct caattaatat tattccatat cctccgggga
     1381 catcttacta taatatttat tgatgagtga ttatgaccaa tattaataca gcttgtgtaa
     1441 aaaataatgc cagttatcaa cttaataacg cattacctaa caaagaaacc atctccagca
     1501 atttttgtga acgactagaa caatggggta ataaatcggt taataatggt gaagaaagag
     1561 caattgccgt ggagcgaatt aaagaagctt acaattcgaa catggcatct cttgatttgt
     1621 catatcttga tttgagtgaa ttaccgccta tcccctcgac agttaatacg ttaaatctgg
     1681 aaaacaattg tctaacttgt cttgacttag ctgacaatgc cagtctcgtt aatatcaacc
     1741 tcagcgttaa taaaattaac acgataacct ttccaaaaga accaaaactg gaaaacattt
     1801 atattaatca taacaatctg gaaagtttag atttaaaaaa tcagcattca ttggttaatc
     1861 tggaagcgca aaataatagc ctaaaaaaat gatatttctg atagttataa actgaaattt
     1921 cttaatcttg attataacaa gctagcatcg ctggatgtct cccggcaaga atctctgatt
     1981 gagttaagtg cccatcacaa tatgatcact gaccttatat tacacaatca tcccaggatg
     2041 aaaacaatca ctttaaacga caaccatatt gcacatttaa acgccaaaaa cactacaaaa
     2101 ctggaatatt taaacttaag caataacaat ttactgccaa ccaatgacat tgatcaacta
     2161 atatcatcaa aacatctttg gcatgtatta gttaacggca tcaacaatga tccacttgca
     2221 caaatgcagt actggactgc agtaagaaat ataattgatg acactaatga agtgaccatt
     2281 gatttatcag gacttaattt aaccactcaa ccaccagggc tgcaaaactt cacctctatc
     2341 aatcttgata ataaccaact cacacatttt gatgcaacca actacgatag actcgtaaag
     2401 ctaagtctga atagtaatgc tcttgagtca ataaattttc ctcaaggcag aaatgtaagt
     2461 attacacata tatctatgaa taataatgct ctcagaaata ttgatataga taggctttca
     2521 tcagttactt attttagtgc ggcacataat caactagagt ttgtgcaatt agaatcttgc
     2581 gaatggctgc aatacctgaa tctcagccat aatcaattaa ctgatattgt tgcaggtaat
     2641 aaaaatgaac tcttactgct ggatctatcc cataataaac taacaagttt acacaatgtc
     2701 ttatttccca acttgaatac gttacttatt aacaacaatt tgctttctga aattaaaata
     2761 ttctatagca acttccgcaa tgttcagaca ttaaacgctg ctaacaacca gttgaaatat
     2821 ataaatcttg atttcctgac ttatcttcca tctatcaaaa gtttaagact ggacaataat
     2881 aaaataaccc acattgatac taataataca tccgatattg gaactttatt ccccataata
     2941 aaacagagca aaaacttaaa ttttttaaat gtttctggga agaacaattg acctactatg
     3001 cagctcatgt tatttaattt gttttcccca gcacttaagc ttaatactgg cctggcaatt
     3061 ctttcgcctg gtgcatttga agttcactct gacggaatag atgcggataa cgaattgttt
     3121 cactatacta ttaaaaaagc atatacccca tataatatac acacttacaa gacagaggaa
     3181 gttgtaaacc agaggaatat aaaagttaaa aacatgacct
//
LOCUS       DAEBXN010000248         3194 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.251, whole genome shotgun sequence.
ACCESSION   DAEBXN010000248 DAEBXN010000000
VERSION     DAEBXN010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.251"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            174..1277
                     /gene="proB"
                     /locus_tag="KJL99_004365"
     CDS             174..1277
                     /gene="proB"
                     /locus_tag="KJL99_004365"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HBD4438842.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRRNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            1289..2542
                     /gene="proA"
                     /locus_tag="KJL99_004366"
     CDS             1289..2542
                     /gene="proA"
                     /locus_tag="KJL99_004366"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HBD4438843.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            2657..2732
                     /locus_tag="KJL99_004367"
     tRNA            2657..2732
                     /locus_tag="KJL99_004367"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2690..2692,aa:Thr,seq:cgt)
     gene            complement(2747..>3194)
                     /locus_tag="KJL99_004368"
     CDS             complement(2747..>3194)
                     /locus_tag="KJL99_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBD4438844.1"
                     /translation="ESKSNRAIGVALNDTACRVLKKQIGNHHRWVFVYKESCTKPDGT
                     KAPTVRKMRYDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVLQEMGGWE
                     SIEMVRRYAHLAPNHLTEHARQIDLILNPSVPNLSQSRNKEGTNDV"
BASE COUNT          756 a          807 c          878 g          753 t
ORIGIN      
        1 aaaatgtggt aatttattaa atctgtaata aaagcgtaaa caactgccgc tacgcttgct
       61 gatcccgcgc aacaaaacgc catgctttgc tcgcagatgg ttggcaaccg acgacagtcc
      121 tgctaaaacg ttcgtttgat atcatttttc ctaaaattga atggcagaga atcatgagtg
      181 acagccagac gctggtggta aaactcggca ccagtgtgct aacaggcgga tcgcgccgtc
      241 tgaaccgtgc ccatatcgtt gaacttgttc gccagtgcgc gcagttacat gccgccgggc
      301 atcggattgt tattgtgacg tcgggcgcga tcgccgccgg acgtgagcac ctgggttacc
      361 cggaactgcc agcgactatc gcctcgaaac aactgctggc ggcggtaggg cagagtcgac
      421 tgattcaact gtgggaacag ctgttttcga tttatggcat tcacgtcggg caaatgctgc
      481 tgacccgtgc tgatatggaa gaccgtgaac gcttcctgaa cgcccgcgac accctgcgtg
      541 cgttgctcga taacaatatc gttccggtaa tcaatgagaa cgatgctgtc gctacggcag
      601 agattaaggt cggcgataac gataaccttt ctgcgctggc ggcgattctg gcgggtgccg
      661 ataaactgtt gctgctgacc gatcaaaaag gtttgtatac cgctgatccg cgcagaaatc
      721 cgcaggcaga actgattaaa gatgtttacg gcattgatga cgcactgcgc gcgattgccg
      781 gtgacagcgt ttcaggcctc ggaactggcg gcatgagtac taaattgcag gccgctgacg
      841 tggcttgccg tgcgggtatc gacaccatta ttgccgcggg cagcaagccg ggcgttattg
      901 gtgatgtgat ggaaggcatt tccgtcggta cgctgttcca tgcccaggcg actccgcttg
      961 aaaaccgtaa acgctggatt ttcggtgcgc cgccggcggg tgaaatcacg gtagatgaag
     1021 gggcaactgc cgccattctg gaacgcggca gctccctgtt gccgaaaggc attaaaagcg
     1081 tgactggcaa tttctcgcgt ggtgaagtca tccgcatttg caacctcgaa ggtcgcgata
     1141 tcgcccacgg tgtcagtcgt tacaacagcg atgcattacg ccgtattgcc ggacaccact
     1201 cgcaagaaat tgatgcaata ctgggatatg aatacggccc ggttgccgtt caccgtgatg
     1261 acatgattac ccgttaagga gctggccgat gctggaacaa atgggcattg ccgcgaagca
     1321 agcctcgtat aaattagcgc aactctccag ccgcgaaaaa aatcgcgtgc tggaaaaaat
     1381 cgccgatgaa ctggaagcac aaagcgaaat catcctcaac gctaacgccc aggatgttgc
     1441 tgacgcgcga gccaatggcc ttagcgaagc gatgcttgac cgtctggcac tgacgcccgc
     1501 acggctgaaa ggcattgccg acgatgtacg tcaggtgtgc aacctcgccg atccggtggg
     1561 gcaggtaatc gatggcggcg tactggacag cggcctgcgt cttgagcgtc gtcgcgtacc
     1621 gctgggggtt attggcgtga tttatgaagc gcgcccgaac gtgacggttg atgtcgcttc
     1681 gctgtgcctg aaaaccggta atgcggtgat cctgcgtggt ggtaaagaaa cctgtcgcac
     1741 taacgcggca acggtggcgg tgattcagga cgccctgaaa tcctgtggct taccggcggg
     1801 tgccgtgcag gcgattgata atcctgaccg tgcgctggtc agtgaaatgc tgcgtatgga
     1861 taaatacatc gacatgctga tcccgcgtgg tggcgctggt ttgcataaac tgtgccgtga
     1921 acagtcgaca atcccggtga tcacaggtgg tataggcgta tgccatattt acgttgatga
     1981 aagtgcagag atcgctgaag cattaaaagt gatcgtcaac gcgaaaactc agcgtccgag
     2041 cacatgtaat acggttgaaa cgttgctggt gaataaaaac atcgccgata gcttcctgcc
     2101 cgcattaagc aaacaaatgg cggaaagcgg cgtgacatta cacgcagatg cagctgcgct
     2161 gacacagttg caggcaggcc ctgcgaaggt ggtggcggtt aaagccgaag agtatgacga
     2221 tgagtttctg tcattagatt tgaacgtcaa aatcgtcagc gatcttgacg atgccatcgc
     2281 ccatattcgt gaacacggca cacaacactc cgatgcgatc ctgacccgcg atatgcgcaa
     2341 cgcccagcgt tttgttaacg aagtggattc gtccgctgtt tacgttaacg cctctacgcg
     2401 ttttaccgac ggcggccagt ttggtctggg tgcggaagtg gcggtaagca cacaaaaact
     2461 ccacgcgcgt ggcccaatgg ggctggaagc actgaccact tacaagtgga tcggcattgg
     2521 tgattacacc attcgtgcgt aaataaaacc gggtgatgca aaagtagcca tttgattcac
     2581 aaggccattg acgcatcgcc cggttagttt taaccttgtc caccgtgatt cacgttcgtg
     2641 aacatgtcct ttcagggccg atatagctca gttggtagag cagcgcattc gtaatgcgaa
     2701 ggtcgtaggt tcgactccta ttatcggcac catttaaatc aataagttac acatcattag
     2761 taccttcctt atttcttgac tgggacaaat ttgggaccga tgggttcaga atcaagtcta
     2821 tttgccgtgc gtgttcggta aggtgattag gtgcgaggtg agcatatcga cgaaccattt
     2881 cgatagactc ccagcctccc atttcctgta acactgataa cgggactccg gcttgaacca
     2941 gccaacttgc ccaggtgtgt ctcaagtcgt gaaatctgaa atcatcaata ccagcccgtc
     3001 tcagcgccgc tttccaggct gtgtttgcgt cataccgcat cttccttact gttggcgctt
     3061 tcgttccgtc tggtttggta cagctttcct tgtacacaaa tacccaacgg tgatgatttc
     3121 cgatttgttt tttcaatacg cgacatgcag tatcattcag cgcaacgcca attgcgcggt
     3181 ttgatttact ctct
//
LOCUS       DAEBXN010000249         3194 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.252, whole genome shotgun sequence.
ACCESSION   DAEBXN010000249 DAEBXN010000000
VERSION     DAEBXN010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.252"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..875
                     /locus_tag="KJL99_004369"
     CDS             <1..875
                     /locus_tag="KJL99_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBD4438845.1"
                     /translation="ITESKIYSAMQKMTNRRHEENWRLRAEACRKKGKPVPEYTPKPA
                     SVATKATHLSFIKALLRAAEREWKMLDKAPIIKVPQPKNKRIRWLEPHEAQRLIDECP
                     EPLKSVVEFALATGLRRSNIINLEWQQIDMQRRVAWINPEESKSNRAIGVALNDTACR
                     VLKKQIGNHHRWVFVYKESCTKPDGTKAPTVRKMRYDANTAWKAALRRAGIDDFRFHD
                     LRHTWASWLVQAGVPLSVLQEMGGWESIEMVRRYAHLAPNHLTEHARQIDSILNPSVP
                     NSSQSKNKEGTNDV"
     gene            1224..2396
                     /locus_tag="KJL99_004370"
     CDS             1224..2396
                     /locus_tag="KJL99_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HBD4438846.1"
                     /translation="MHMIEINSLLLITSVILMSLLAVGLFDKISPINLVEHGRNNQID
                     GMRGFLAIFVLIHHAAIWNGYLSSGVWEAPSSNLLANLGQVGVSFFFMITGYLFFSKI
                     ISGDQDWTRLYVSRLLRLTPMFIVSLCLIFIIVGFKSGWRMQVSTEELFVSIMKWLPF
                     TALGMPNINDVKDSFTINAAVTWTLVYEWFFYFSLPVISALIKRKVSIYMVMISAISL
                     FVFILFFSKIHIASFLFGLLAFLLNKSKIVNGIAKAKVTPIIITAIMVFEMTYFKTTY
                     APLPLILCGITFIIIASGCDLYGILRLNITRKLGETTYSVYLLHGIFLYCLMTWIIPN
                     NYTENTFIILVSTTAFLITFTSCLTFKLIETPFIKLTKQTTTLVKELIPTLTNNNQ"
     gene            complement(2450..3073)
                     /locus_tag="KJL99_004371"
     CDS             complement(2450..3073)
                     /locus_tag="KJL99_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBD4438847.1"
                     /translation="MVLNNPYFEANAGGADLAIDNTGTRPVTVVINGGNFHRVSSARY
                     THTNIQVTSSGGGKVTVILNGTTFQSAGDYQPSAERPYWITGANCEVVDIGCVFTETT
                     SKVTSASALSVTRSGKINANGSIDVATGVSSVNVVSTGVYDVSFSHPLAAVASGYIVQ
                     ITPISAPDSVSCDVTYIGVDTFRVTLRNTLSGAGISSSFAFSITRLI"
BASE COUNT          999 a          666 c          638 g          891 t
ORIGIN      
        1 acattactga atcaaaaatt tattcagcaa tgcagaaaat gacgaaccgg cgtcatgagg
       61 aaaactggag actcagggca gaagcatgca gaaaaaaagg gaaacctgtt ccagaataca
      121 cgccaaaacc agcgtccgtt gcaacgaagg ctacgcatct ttcatttata aaggccctac
      181 taagagccgc agagcgtgaa tggaaaatgc tggataaggc accaattatt aaagtgcctc
      241 aaccaaagaa taaacggatc cgctggctgg agccccatga agcacaaagg ctgattgatg
      301 aatgtccgga gccattaaag tctgttgttg aatttgcact ggcaacaggc ttaagacgct
      361 cgaacatcat caaccttgaa tggcaacaaa tagatatgca gcgccgggtg gcatggataa
      421 acccggaaga gagtaaatca aaccgcgcaa ttggcgttgc gctgaatgat actgcatgtc
      481 gcgtattgaa aaaacaaatc gggaatcatc accgttgggt atttgtgtac aaggaaagct
      541 gtaccaaacc agacggaacg aaagcgccaa cggtcaggaa gatgcggtat gacgcaaaca
      601 cagcctggaa agcggcgctg agacgggctg gtattgatga tttcagattt cacgacttga
      661 gacacacctg ggcaagttgg ctggttcaag ccggagtccc gttatcagtg ttacaggaaa
      721 tgggaggctg ggagtctatc gaaatggttc gtcgatatgc tcaccttgca cctaatcacc
      781 ttaccgaaca cgcacggcaa atagactcga tcctgaaccc atcggtccca aattcgtccc
      841 agtcaaaaaa taaggaaggt actaatgatg tgtaacttat tgatttaaat ggtgccgata
      901 ataggagtcg aacctacgac cttcgcatta cgaatctgta gcaccaatca taactatctg
      961 ttttagcaag cattaaccgc attcactaag ctatagttga tgacacaaac agaaagttga
     1021 tgcatgatgt tgccatatat atgtcacaaa tacggcacaa cgatcttcaa acatatagcc
     1081 acacattcac agaagagcac aaagccttgc aatccagtgc aaagcttggt gtgtctccgt
     1141 tttgtttcat cagttacaca taactatatg atcattaact aaaagtcagt atacttggag
     1201 aaattttaat tgcgtaatag agcatgcata tgattgaaat taacagttta ttattaataa
     1261 catccgtgat attgatgtcg ttattagctg tggggttatt tgataaaatt tccccaataa
     1321 atcttgttga gcacggcagg aataatcaaa tagatggaat gcgggggttc ttagcaattt
     1381 tcgtgcttat tcatcacgca gcaatttgga atggatactt gtcatctgga gtatgggaag
     1441 caccttcatc aaatctgtta gcaaatttag gccaagttgg tgtgtcattc ttttttatga
     1501 ttactggtta tctgttcttt tcaaagatta tctctggaga tcaggactgg acaaggcttt
     1561 atgtatcaag attactacga ttaaccccaa tgttcatagt tagcctatgt ctcattttta
     1621 tcattgtagg ttttaagtct ggatggagaa tgcaggtatc cacagaagag ctttttgtgt
     1681 caataatgaa gtggttgcca ttcactgcgc taggtatgcc gaacattaat gacgtaaaag
     1741 attcatttac tatcaatgcc gctgtaacat ggacacttgt atatgaatgg ttcttttatt
     1801 tttctcttcc ggtaatttcc gcgctcataa aaagaaaagt cagtatttac atggttatga
     1861 ttagcgcaat atcgctattt gttttcattt tatttttcag taaaatacac atagcatcat
     1921 ttctttttgg gctcctggca tttttgctaa ataaatcaaa gatagtcaat ggaattgcca
     1981 aagcaaaagt aacaccaatt ataataactg cgataatggt ttttgaaatg acgtatttca
     2041 aaacaactta cgcaccgcta ccactgattc tttgtggaat aacatttatc attatcgcat
     2101 caggttgtga tttgtatgga atattaagat taaatataac cagaaagcta ggagaaacta
     2161 cctacagcgt ttaccttctg catgggatat tcctttattg cctaatgacg tggattattc
     2221 ctaataatta cactgaaaat acatttatca tactggtaag cacaactgca tttcttatta
     2281 cgtttacatc atgcctaact tttaaattaa tagagacgcc attcatcaaa ttaacaaaac
     2341 aaaccacaac tttagtaaaa gaattaatac ccacattaac aaacaacaat caataaacaa
     2401 taaacataag ccgccctaca tatatgtcca aatggggcgg cgaatgtatt tatattagtc
     2461 tggtaatgct aaatgcaaac gaactggaga ttcctgcccc gctaagcgta ttgcgcaggg
     2521 tcaccctaaa tgtatctacg ccaatataag taacatcaca actaactgaa tcaggcgcag
     2581 atataggtgt tatctgcaca atataaccgc tagcaactgc tgcgagcgga tgagaaaagc
     2641 taacatcata cacacctgta gaaaccacgt taaccgatga aacaccagta gcaacgtcga
     2701 tactaccatt ggcgttaatt tttcccgagc gtgtgactga caatgcagaa gccgatgtta
     2761 ccttactggt tgtttctgta aaaacacacc caatgtcaac tacctcgcaa ttagctcctg
     2821 ttatccagta tggtcgttct gcacttggct gataatcccc tgcgctctgg aacgttgtgc
     2881 cgttgagtat gactgtaact tttcccccac ctgagcttgt tacttgaata ttcgtgtgcg
     2941 tgtatcttgc actgctcacg cgatgaaaat ttccaccgtt tataaccaca gttacagggc
     3001 gcgtcccagt attatctata gcaaggtctg cccccccggc attagcctca aagtatgggt
     3061 tgtttaatac caaagcgcac gaaccgttaa ggccatttat agctaattgc gcaccacctg
     3121 tactggaatc cccctgagta ccatttcctt cacaagttga ggtgtactgg caatagcgga
     3181 cactaccatt tgtt
//
LOCUS       DAEBXN010000250         3190 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.253, whole genome shotgun sequence.
ACCESSION   DAEBXN010000250 DAEBXN010000000
VERSION     DAEBXN010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3190
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.253"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            223..882
                     /locus_tag="KJL99_004372"
     CDS             223..882
                     /locus_tag="KJL99_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HBD4438848.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     gene            complement(1083..1460)
                     /locus_tag="KJL99_004373"
     CDS             complement(1083..1460)
                     /locus_tag="KJL99_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBD4438849.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(1527..>3190)
                     /locus_tag="KJL99_004374"
     CDS             complement(1527..>3190)
                     /locus_tag="KJL99_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HBD4438850.1"
                     /translation="GFIKPRWQKLVMTDAGIDRRYYELCALSELKNSLRSGDIWVQGS
                     RQFKDFEDYLVPPEKFTSLKQSSELPLAVATDCEQYLHERLTLLEAQLATVNRMAAAN
                     DLPDAIITESGLKITPLDAAVPDTAQALIDQTAMVLPHVKITELLLEVDEWTGFTRHF
                     THLKSGDLAKDKNLLLTTILADAINLGLTKMAESCPGTTYAKLAWLQAWHTRDETYST
                     ALAELVNAQFRHPFAGHWGDGTTSSSDGQNFRTASKAKSTGHINPKYGSSPGRTFYTH
                     ISDQYAPFHTKVVNVGLRDSTYVLDGLLYHESDLRIEEHYTDTAGFTDHVFALMHLLG
                     FRFAPRIRDLGDTKLYIPKGDAAYDALKPMIGGTLNIKHVRAHWDEILRLATSIKQGT
                     VTASLMLRKLGSYPRQNGLAVALRELGRIERTLFILDWLQSVELRRRVHAGLNKGEAR
                     NALARAVFFNRLGEIRDRSFEQQRYRASGLNLVTAAIVLWNTVYLERAAHALRGNGHA
                     VDDSLLQYLSPLGWEHINLTGDYLWRSSAKIGAGKFRPLRPLQPA"
BASE COUNT          687 a          827 c          920 g          756 t
ORIGIN      
        1 gacgcacttt gcgccgaata aatacctgtg acggaagatc acttcgcaga ataaataaat
       61 cctggtgtcc ctgttgatac cgggaagccc tgggccaact tttggcgaaa atgagacgtt
      121 gatcggcacg taagaggttc caactttcac cataatgaaa taagatcact accgggcgta
      181 ttttttgagt tatcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact
      241 ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag
      301 tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag
      361 accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg
      421 atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat
      481 agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg
      541 agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt
      601 tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca
      661 gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc
      721 ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg
      781 ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggcag aatgcttaat
      841 gaattacaac agtactgcga tgagtggcag ggcggggcgt aattttttta aggcagttat
      901 tggtgccctt aaacgcctgg ttgctacgcc tgaataagtg ataataagcg gatgaatggc
      961 agaaattcga aagcaaattc gacccggtcg tcggttcagg gcagggtcgt taaatagccg
     1021 cttatgtcta ttgctggttt accggtttat tgactaccgg aagcagtgtg accgtgtgct
     1081 tctcaaatgc ctgaggccag tttgctcagg ctctccccgt ggaggtaata attgacgata
     1141 tgatcattta ttctgcctcc cagagcctga taaaaacggt tagcgccggg gttggatttt
     1201 tcagcgttcc agctaaggct aaggcattcc tgttcaagcg caagccgggc tataaagcgc
     1261 attatcgctt tacccgtgcc cttatttcga tcgcactgag aaacatacag ctctttaata
     1321 tgcagctgac cgctgtatcg gggcgaggga taaagaatat tgcaacatgc caggccagta
     1381 atgttattgc cgcagcgcgc tctgatcacc agggtaccgg aaagccggtt gaataacttt
     1441 ttacagagat aatccttcat caacgcttcc tgaatgatac cttcgccata ataggggtcg
     1501 tctcagaaaa cggaaaataa agcacgctaa gccggttgca gaggccgtag cggcctgaac
     1561 ttccccgcgc cgatcttggc gctgctgcgc cataggtaat caccggtcag gttgatgtgc
     1621 tcccagccga gtggcgacag gtactgcaat agcgagtcat cgacggcatg accattgccg
     1681 cgcaacgcat gcgccgcacg ctccaggtag accgtgttcc acagcacgat ggccgccgtc
     1741 accaggttga ggccgctggc ccggtagcgc tgctgctcga aactgcggtc acggatttca
     1801 ccaaggcggt tgaagaacac ggcacgggcc agcgcattgc gcgcctcgcc cttgttcagc
     1861 ccggcatgca cgcggcggcg tagctcgacg ctttgcagcc agtcgaggat gaacagcgtg
     1921 cgctcgatgc ggcccaactc gcgcagcgcg acggccaagc cgttctggcg cgggtagctg
     1981 ccgagtttcc tgagcatcag cgaggccgtc accgtgccct gcttgatcga ggtggccagc
     2041 cgcaggattt cgtcccaatg ggcgcggacg tgcttgatgt tgagcgtgcc gccgatcatc
     2101 ggcttgagcg cgtcataggc ggcatcgccc ttcgggatgt agagcttggt gtcgcccagg
     2161 tcgcggatgc gcggcgcgaa gcggaagccc aagaggtgca tcagggcgaa gacgtgatcg
     2221 gtgaagcccg ccgtgtcggt gtagtgctcc tcgatccgca ggtcggattc gtggtacagc
     2281 aggccgtcga gcacgtaggt tgagtcgcgc aggccgacat tgaccacctt ggtgtggaat
     2341 ggcgcgtatt ggtcggagat gtgggtgtag aaagtccgtc ctgggctgct gccatatttt
     2401 gggttgatgt gccccgtgct ctttgccttg ctagcggttc ggaaattctg tccgtccgat
     2461 gatgatgtgg tgccatcgcc ccagtgcccg gcaaagggat gccgaaactg agcgttgacc
     2521 agttcagcca acgctgtcga gtacgtttcg tcgcgggtat gccaggcttg cagccaagcg
     2581 agcttcgcgt aggtcgtgcc ggggcaggac tcggccatct tggtcaggcc caggttgatc
     2641 gcgtcggcca ggatcgtggt caacaacagg ttcttgtcct tggccagatc gcccgatttc
     2701 aagtgcgtga agtgccgggt gaagcccgtc cactcatcga cttcgagcag cagttcggtg
     2761 atcttgacgt gcggcaggac catggctgtc tggtctatca gcgcctgcgc ggtgtcgggc
     2821 accgccgcat ccagcggcgt gatcttcaag cccgactcgg tgatgatggc atccggcagg
     2881 tcgttggctg ccgccatgcg gttgacggtg gcaagttgtg cttccagcag cgtcagccgc
     2941 tcatgcagat attgttcgca gtcggtggcc acggccagcg gcaattcgct ggactgcttg
     3001 aggctggtga acttctcggg cggtaccagg tagtcctcga agtccttgaa ctggcgtgaa
     3061 ccctgcaccc agatgtcgcc cgagcgcagg gagttcttca actcggacag cgcgcacagt
     3121 tcgtagtagc gccggtcgat gccggcgtcg gtcatcacca gtttctgcca gcgcggcttg
     3181 atgaagccgg
//
LOCUS       DAEBXN010000251         3181 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.254, whole genome shotgun sequence.
ACCESSION   DAEBXN010000251 DAEBXN010000000
VERSION     DAEBXN010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3181
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.254"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            419..1528
                     /gene="fepE"
                     /locus_tag="KJL99_004375"
     CDS             419..1528
                     /gene="fepE"
                     /locus_tag="KJL99_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HBD4438851.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVI
                     LFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVGFDINRDSAFA
                     LFIKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKN
                     DKSSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEI
                     LALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIA
                     TKLNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPS
                     NAIIVILSSVIGGMLACGYILLRHAMEARKMSVIE"
     gene            complement(2553..2747)
                     /locus_tag="KJL99_004376"
                     /pseudo
     CDS             complement(2553..2747)
                     /locus_tag="KJL99_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
BASE COUNT          801 a          659 c          776 g          945 t
ORIGIN      
        1 aaaaaaaaac attatcttat attaggggtg ctgctagcgg cgcggtgtgt ttttttacag
       61 gacgcccctg ggggcgctgc taggggtgtc tgttcagata tgtagcaatg agtcagggat
      121 tcacgaactg aaaatgtccg aaaatgtgtc tcatgggcac tgtatgcgcc caaaccgatc
      181 gtcattcact ttcatgcata gctatgcagt gagctgaaag cgatcctgac gcatttttcc
      241 ggtttacccc ggggaaaaca tctctttttg cggtgtttgc ggcagaatcg cgttcagcgc
      301 gttttagcgg tgcgcgcaat aaaatgttat gttaaatgtc tgctgaatgg tttttaggtt
      361 ttttcctaac atatgataga ataaaaaaac catatgttaa tatattgtgt ttttttcaat
      421 gaaaatctca aaggtctctg acgaacattt ttctaattac aacatgttgc cgaatggtaa
      481 tgaagaaatt gacctttttg gcttgattag cgttttatgg gcagcaaaaa taaaaattgt
      541 tggggtgatt ctattttttg cttgtattgg ctttcttgtt tcttttgttt tgccacaaaa
      601 atggactagt tctgctgtgg ttactcctgc tgagtctgtt caatggagtg aattaaataa
      661 ggagctttcc aagcttcatg ttctgggagt gggttttgat atcaatcgtg atagcgcatt
      721 tgctctgttt attaagaaat ttcagtccgt taattcgttg aatgagtata tgagatcatc
      781 tccttatgtt atggagatta ttaaaaagaa taacattagc gctctcgaac tgcatcgtgc
      841 aattgttgag ttgagtaaaa atatgaagtc gattgatgat aatgccagta agaaaaacga
      901 caagtcatca ttgtatgtat catggactct gagttttact gctccaacaa gtaaagaagc
      961 tcacgatgtg ttgtctgggt atattaatta tgtttcttcc cttgttgtaa gggatttgat
     1021 ggaagatata agaaataaac tagaagttaa aactaatgtt gaaaaagaaa ttcttgcact
     1081 ggatgagata aaaattagaa accagctgaa tgcagatatt cgacgcctca attattcact
     1141 ggaggttgct aatgcggctg gaataaaaaa acctgtatac agcaatggtc agattatgaa
     1201 ggatgaccca gattttcctg tggctctcgg ttctgatggt atagcaacta aattgaacat
     1261 caaaaaatca atcaaggatg tttcggaatt gagtggggag ttgcgaaatc gtcaatatgt
     1321 tgtgaatcaa ttggttgtgg cgaaagtggg ggatgttgat tttatgccat ttcagtatca
     1381 attgagccca acgttaccgg tcagaaagga tgggccaagt aatgccataa ttgtaattct
     1441 ttcttctgtc attggaggga tgcttgcatg tggctatata cttttacgtc atgccatgga
     1501 ggccagaaag atgagtgtga ttgagtaaaa gttgtgctga tgcatcattt tataagcaac
     1561 ttttataaaa agttcaaagg atcttcttga gatcattccc ttacagccgt agtcttctac
     1621 tctagaaaac gaaaaaacca cctggggagg tggtttttcg aaggttcagt aagttgggga
     1681 acttctgaac cgtggtaaca gtcttgtgcg agacatgtca ccaaatctgt ccttctagtg
     1741 tagcctcagt aaggctgcca cttcaagaac tctcaataca tctctcgcac atcctgttta
     1801 ccagtggccg ctgccaatgg cgttaagtcg tgtcttaccg ggttggactc aagacgatag
     1861 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     1921 gagcgaactg cctacccgga actgagtgtc aggcgtggaa tgagacgaac gcggccataa
     1981 cagcggaatg acaccggtaa accgaaaggc aggaacagga gagcgcacga gggagccacc
     2041 agggggaaac gcctggtatc tttaagtcct gtcgggtttc gccacctctg acttgagcgt
     2101 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccac cggcgcggcc
     2161 ttttttcggg gcctcttttt tgctcacatg ttttttctgg tgttatcccc tgattctgtg
     2221 gataaccgta tatccgtctt tgcgtgagca gacaccgctc gccgcagccg aacgaccgag
     2281 cgcagcgagt cagtgagcga ggaagcggaa gagcgccgga agctgcattt tctccttacg
     2341 tatctgtgca gcatttcaca cctcatatgt cgctgttctc tcaccatctg ctctgccgcc
     2401 tcataattaa gccagtatac actccgttat cgctacgtga ctgggtcatg gctgcgcccc
     2461 gacacccgct aaaaccccct gacgcgccct gacgggcttg tctgctgacg aaaacgccca
     2521 cgggaaagcc gtgaccgcct ccgtcagctt cgttatgttc ctgtttcact ttcggcctca
     2581 aagcgaatct ggatgctgtt ctggagttct tctgcgagtt cgtgcagtcg tttgcacatg
     2641 gccgcctgtt cgtcggcatc cagagcatcc agtttttcaa gcagtgtcag gctctggctt
     2701 ttgatacatc gcgccatatt cagcgcagtt tgttgttatt tgttcacctt ccgttgttct
     2761 ccgtcagttc cgggtgtcgg ggggggggac cctgagcagg tggtaattgt aagacgttgc
     2821 gggcgacggt attacaattg cacatcctgt cctgtttctt taggacgtat catggcacag
     2881 catatcaact gataaataat ccctgtgcag tgcagccgct gttgcataaa atttcgctct
     2941 gcgtcgagtg tttcttaatc aataattccg ctgatactcc ggaaaaacgc gagtctgttc
     3001 tcatgcctga aatgcccaca ccccacgcaa aaacaagttt ttgctgattt ttctttataa
     3061 atagagagtt atgacaaatt agttcttctt gctctctttg tgatatttaa aaaagcggtg
     3121 tcggcgcggt gttgtagctg cgccaacacc gctttttagg ggtggtactg actattttca
     3181 t
//
LOCUS       DAEBXN010000252         3112 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.255, whole genome shotgun sequence.
ACCESSION   DAEBXN010000252 DAEBXN010000000
VERSION     DAEBXN010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.255"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(<1..709)
                     /gene="ipaH9.8"
                     /locus_tag="KJL99_004377"
     CDS             complement(<1..709)
                     /gene="ipaH9.8"
                     /locus_tag="KJL99_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HBD4438852.1"
                     /translation="MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERD
                     EAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKL
                     YSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALK
                     NLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSS
                     HALQALQRLTSSPDYHGP"
     gene            complement(971..1066)
                     /locus_tag="KJL99_004378"
                     /pseudo
     CDS             complement(971..1066)
                     /locus_tag="KJL99_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1102..1407)
                     /gene="ccdB"
                     /locus_tag="KJL99_004379"
     CDS             complement(1102..1407)
                     /gene="ccdB"
                     /locus_tag="KJL99_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HBD4438853.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1409..1627)
                     /gene="ccdA"
                     /locus_tag="KJL99_004380"
     CDS             complement(1409..1627)
                     /gene="ccdA"
                     /locus_tag="KJL99_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HBD4438854.1"
                     /translation="MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERW
                     QAENQQGMAEIARFIEMNGSFADENRDW"
     gene            complement(2163..3112)
                     /locus_tag="KJL99_004381"
                     /pseudo
     CDS             complement(2163..3112)
                     /locus_tag="KJL99_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          773 a          753 c          757 g          829 t
ORIGIN      
        1 gtgggccgtg gtagtccggc gaagaggtta atctttgcag ggcttgcaga gcatgggatg
       61 ataatgggtt atcactaata tgtattgaac attcattcct cagattaaga atgctttccg
      121 ggatatgact tatctgattt ctatcaaaaa aatactctct gacaacggga ttatttccct
      181 cagaaaatgc tggtagctca gtgaggaaat tacgggtcgc tctgagattt ttaagagcct
      241 gtgggagcga tggtaaggag actatttcgt tatagctgat attcatagtc aataacgaat
      301 cgggtaaagc tggcaggttt tccagctcat tgtgttgtac ctgaagtgac tccagcgcag
      361 gaggtagcac gggcaattct gataatttat tgctggcgga atataatttt tttagcgtaa
      421 caggcagttc aggtaggtta gttaattgat tatatgatac attgagcagc gttatctgag
      481 ctggtaagtt gtcaggtagc gaggacagat ttaaacggtc cagtcgaagt tcatcggaat
      541 tattgataag acattcttta agtcgggaga cagcctcatc acgctcttca ccggggagcg
      601 cttgtttttc ccatttatcc catgctgaaa agtaatcagc atatgtaccg gaaatagtgt
      661 tataaaaaga attttggggc aatgaaaagt tattatttat cggtaacatg atgggcatcc
      721 agttaaagcc ggtggacaaa tggctttgtt aaaagaaata agtaggagtt tttatgaagt
      781 tcattccatg aaatgaactt cagcactttt ttattataat aaggataaac aaaattggct
      841 gtaaaaaaac gatactgttt cagttaaccg tgaatggcct gtttgcaccg tattgttctt
      901 gtttttcggt tagttaaaca tcgtacttca tatttgaacg gtaagcggct cgtcagaacc
      961 gtattgatat ttactgagag gtcagatcaa ctttccaagg caacagatcg cgtacccggt
     1021 ttgccggcca gtcctggata tgctcaatga cgtaacgcag ccattttgtc aacgccacga
     1081 tttttgaccc tagcctgaca tttatattcc ccagaacatc aggttaatgg cgtttttgat
     1141 gtcattttcg cggtggctga gatcagccac ttcttccccg ataacgaaga tcggcacact
     1201 ggccatatca gtggtcatca tgcgccagct ttcatccccg acatgcacca ccgggtaaag
     1261 ctcacgggag actttatctg acagcaggcg ggcactggcc agggggatca ccatccgtcg
     1321 tccgggcgtg tcaataatgt cactctgtac atccacaaac agacgataac gactctctct
     1381 tttataggcg taaaccttaa actgcatttc accagtccct gttctcatcg gcaaaagagc
     1441 cgttcatttc aataaaacgg gcgatctcag ccatcccctg ctgattttca gcctgccagc
     1501 gttccgcacg cagacggcgg gcctcttttt gcatggccgt atttaccagg cctgagatat
     1561 tcacgtttgc ggatttaagt aactgatagc tgtcactgtc tatagtgaca gtaatacgct
     1621 gcttcatgat aagccctcaa aaacatactt atgatatgta tatgagtata tattaatggt
     1681 acaaaccatc aatatccata aaaagtacac cattaattcg ctataagttt ttaatacaat
     1741 tttttctgct gcgatttttt tatactataa ttagcatact tataacaccg taagtaaatt
     1801 acatgaaagc aggcacagcg tgatccgatt tttctgcgta taacgatttt ccggaagtgt
     1861 aatacggttg cgccccgggc cgggcaccct tttctgttgt tgtgtacggg cccacagaga
     1921 atggtaccgg aggcgggctc agaagtactg agtcgggtga tcgcgtcagg ccgcgaagta
     1981 ctgacacggg tattggtgac tggtgatgaa gtactgcccc gggtatgatt tcaggctgat
     2041 tcaggttctg attctctgtc agtccgcgtg gttgctggcc tggctgattt cctgttgtca
     2101 ggcgttcaac aaccgactgc aggttatagc ccggttcacg aacacctgac ggcaggtatc
     2161 actcacccgg tgaaggtaaa tccctcaaga ggcagcaact ctgccagtcg ctcctccggc
     2221 cattccggca gacgcgtcag gacgtccgtc agccaggcat gtggctccag accattacgt
     2281 tttgctgttt ccagaaggct cattatctgt gccgcccggt ttccggccat cagtgaaccg
     2341 gcgaacagcc acgctttccg tcccataaca accgggcgaa tggcacgctc gcagatattg
     2401 ttgtccaggg gcacagctcc gtcacccagg aagcagctca gttcatcgcg ccggttcaga
     2461 atatagttaa tcgccttatt taatggcccg tcgggcggac agcacggctc ctgctcttca
     2521 agccaggcaa acaggtcatt cactatcggc cgggagtacc gttatcgcca ctgccgtatt
     2581 ttttccacag ggcgctcacg gatgagtttc tcaatgcggt aaagacccgc gatttttctc
     2641 agctccacgc ctgcacgggg ctccctgctg gccttataca gatttgcgaa gccgcgccct
     2701 gcgtgactcc agcaacaggc tgatgtgatg ccggggtgat tatccgccag agatttgtac
     2761 acgctgtatc cgtcactcac cagcgtactg ccacaccagc actgcagcca ggtctcaggg
     2821 tatctgaggg cccgtccggt ctgcgagtca aaacagacga ccggcggacc gcttctttca
     2881 ccgctgacgt aagcacacag ccagccggag caggatttac cgcctttccg ggtatcaagg
     2941 agcctcaggg acgtttcatc tgcatgaata acatcccgtg tcagaagctc atggcgtagc
     3001 agtttcgcca gcggggccag tgcagctccg gcaacaccga ccatatccgc cattgtactg
     3061 acggggagct ccacacccat tcgggagaaa atgtgctgct ggcgatacag gg
//
LOCUS       DAEBXN010000253         3099 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.256, whole genome shotgun sequence.
ACCESSION   DAEBXN010000253 DAEBXN010000000
VERSION     DAEBXN010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.256"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(106..1815)
                     /locus_tag="KJL99_004382"
     CDS             complement(106..1815)
                     /locus_tag="KJL99_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HBD4438855.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIMRVSNSSEL
                     IQFKNKTAPYFSEKRNVEVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLL
                     STPDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNMKKNGDFVRTLSACT
                     LNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDF
                     YWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEK
                     YKNGEISQSKLVNLLATRSSDGTPAFYIALQNGYSDIIQVYGKILNMCNLSQETILTL
                     LAAVGANNVPGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFF
                     ALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKAIMAYGDILKI
                     LPPHQEYIDELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKNYSFTTRRLVEMLSA
                     TNKDGIPGVFVSVVNRDKETILEYCRIIKENNLEPDTIAEQFSKKMKKTFIEIINRFN
                     HFL"
     gene            complement(2247..2966)
                     /locus_tag="KJL99_004383"
     CDS             complement(2247..2966)
                     /locus_tag="KJL99_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HBD4438856.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPTYSDICQSFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
BASE COUNT          953 a          588 c          519 g         1039 t
ORIGIN      
        1 ttgccaattg aggtggttgc ctgaaagttg gcagttaccc ggtcactaca ccttgttgga
       61 gtgaacatat caaaaacagg taattacaca atttaaatta cagtctcata aaaaatgatt
      121 aaatctattt attatttcaa taaacgtttt tttcatcttc tttgaaaatt gttctgctat
      181 agtatccggt tccagattgt tctcttttat tattctacaa tactctaata ttgtttcttt
      241 gtctctattt actacagata caaatacacc aggaatacca tctttattag ttgcggacag
      301 catttcgacc aatcgtcttg tggtaaaact atagtttttt agaatattac aaaaggcacg
      361 tattgtttcg gtatggccat tataaaatgc cataaaaagt cctgatgtac cgttgggatt
      421 ttttatccat aataattcat ctatatattc ctggtgaggg gggagtattt ttagtatatc
      481 tccatacgcc ataatggcct ttgtgtgccc attttgaagt gccataaaca acccatgctt
      541 tactttcaat aattctttaa tttgttcact actaagcatt ttcttattaa gcagacttcc
      601 gaacatacga atactatctg catgtccatt ctgcaatgca aaaaataacc caggtaaatc
      661 atggctgtct ttcgcagcta atagatataa tctcttatcg gaagttaacg gggttttaaa
      721 gactatttcc ccataggctt ttattgtgtc tacatgtccg ttcataaaag acatgcataa
      781 acccggcaca ttgtttgccc ctaccgcagc aagcaatgta agtattgttt cttgactaag
      841 attgcacata tttaatatct taccatatac ttgaataatg tctgaatatc cgttttgaag
      901 tgctatataa aatgcaggag taccatcaga gcttctcgtg gccagaaggt tcaccagttt
      961 agattgagaa atttcaccat ttttgtattt ttccatcagt ataaataata cttctgtcag
     1021 attctttgtt atcgcatgat atataactct ggaattgatc aattcatctg gtaaaagttt
     1081 cactgtgttt gttagagggg aattatgcct atcttcaaat attacctgcc cgggttgctc
     1141 cagaccataa attttccaat aaaaatcatc aaaaaaatcc ttagcggata attgactcag
     1201 ttcgaaatgg ctatcacaag taaatacagt acgtttatga gtaacagttc gattaggatc
     1261 gaaaaactga acaacccatt tttcactttc ttgtacgcgc tttattttca accctaacgc
     1321 catttggtgg tttaatgtgc atgctgataa tgttctgaca aagtctccat tttttttcat
     1381 gttataaaaa aaattagtaa taactgttcc ccacttttta ttttccacaa aataaatata
     1441 agctggtgat tttgaaaaat cttccgtttt ttccaaacat acattttttg caatagcatc
     1501 aggggtgcta agaagctgat agtttacctt cccatttgtt tccataaaat tcatttccca
     1561 ataggaagct aggtgacggc aaactatttg tcttccatat atatcttttg ccactccatt
     1621 tatatttacc tccacatttc tcttttctga aaaataaggt gctgttttat tcttaaactg
     1681 aattagttca ctggagtttg agaccctcat aatagaactt gttattgttc tattatctct
     1741 gttaacagac atatctgttg ataacgaatt ttttgttgaa aatatactag atgaaaatgt
     1801 tttatttaac ggcatagaag acaaccatag agatgttata atactattag attgcagtta
     1861 ctaatagtaa aagcctcctt acaaggaggt agcttattca gaatgctcct ataagatagc
     1921 gagctcgtca acgatacaga tgatctgatt atattgtgca aaaggagaac aggaaaatag
     1981 gcactggaaa agatgaaggc ctgatgggag cattaactaa tgcaaaatcc gacaggttaa
     2041 tatcccgaaa agcaatcaaa cgactcaaac aggagatgca cgctgaaatg gtggacaact
     2101 gttcggattt cagatgagta gctcagcagt ttatgagccg aatgctctgt cacgggcagg
     2161 aatcaggctg catacgcagt gccaaatcta atcgtagtta ttctaaccag ttttcaagct
     2221 acaaggtggt gtagctggca tcttctctac tctatcatca aacgataaaa tggttcctct
     2281 cgtaacattt gctcttgcct ttcacttcca tctcgatcgc ttcgtagttc attacgatag
     2341 ctgacatatt tccagtcttc aggtctaaca tctgacgccg gatactcccc gggagcgatt
     2401 ttgcttctta atagattaga ctcgagacac attataaatt gtcgtatttt atgaagtaat
     2461 aaagcactat attgcgagca ttcctgatct gattttacat atagcgtaaa ctgagctcct
     2521 atccccacac gagattgctg agaaactcga ttcatatcag ttatcttcca tttatctata
     2581 ggactgtcct ctgaaaataa taaaccagac aaaatttgga atgccagagg aacttgctcc
     2641 ctagctatac taatatgaaa cttgtcccca acaaaatccc ccttactctg aggattttca
     2701 cgacatgcat gaataaacac atctccacta tggttcacaa tctggaatcc ttggtacatg
     2761 acagaaaaag attgacatat atcagagtaa gttggcacgt tcgatgctac tttgaaatag
     2821 tacgctgggt attgccgtat ttggttgtac aatatattga agttattttt caatctttca
     2881 ttcgcagaaa gcatctgcgg tatttcagac ctcacaacat tcaaagaatc taaatttagt
     2941 tttagacagg gcttttttat gggcatagaa aacgtcctct ataaaataga tatattatct
     3001 tatattgatt gtttatcatc ttttataaaa cgatacatta tatgtaatgt atgtaagcgt
     3061 aagggtcctc tccgaacatg gtgggaaggc ttagaacgc
//
LOCUS       DAEBXN010000254         3069 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.257, whole genome shotgun sequence.
ACCESSION   DAEBXN010000254 DAEBXN010000000
VERSION     DAEBXN010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.257"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..498
                     /locus_tag="KJL99_004384"
     CDS             <1..498
                     /locus_tag="KJL99_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBD4438857.1"
                     /translation="DGEILKSACEINDSDKKIEVALGHYNAEQFRSVGDRSPKIPFTI
                     PLVNCPVTGWEHDNGNVEASFRLWLETRDNGTVPNFPNLAKVGSFAGTATTGVGIRID
                     DAESGNLMPLNAMGNDNTIYQIPADSNGIVNVDLIAYYVSTVEASEITPGEADAVVNV
                     TLDYR"
     gene            578..1273
                     /locus_tag="KJL99_004385"
     CDS             578..1273
                     /locus_tag="KJL99_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HBD4438858.1"
                     /translation="MSKRTFAVILTLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGKSRAPFIITPPLFRLEASDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRVKMFYRPAHLKGRVNDAWKSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1302..>3069
                     /locus_tag="KJL99_004386"
     CDS             1302..>3069
                     /locus_tag="KJL99_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBD4438859.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPSTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYAFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWGYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNSNISVGVSGQQWGIGYSLNYQYSRYTDENNDRALSL
                     NLSIPLERWWICPYISRHLLSLN"
BASE COUNT          889 a          718 c          683 g          779 t
ORIGIN      
        1 gatggcgaga ttttaaaatc cgcttgtgaa atcaatgact ctgataagaa aattgaagtt
       61 gctcttggtc actataatgc cgaacagttt cgttctgttg gcgatcgtag cccaaaaatt
      121 ccattcacta ttcctttagt taactgccca gtgactgggt gggagcacga taacggcaac
      181 gttgaagcgt ctttccgttt atggctggaa acgcgggata atggcaccgt ccctaacttc
      241 cctaatctgg caaaagtggg ctcttttgct ggcacagcga caacaggtgt gggtatccgt
      301 attgacgatg cggaaagcgg aaaccttatg ccactgaatg ctatggggaa tgataacacg
      361 atttatcaga tcccagcgga ctccaatggt attgtcaatg ttgacctcat cgcttactac
      421 gtatcaaccg ttgaagcatc agaaatcacc ccaggggaag cagacgctgt cgttaacgta
      481 acgctggatt accgttaata cgttacggcg ttatctgacc tttcagataa cgcccttttc
      541 cttcctcttt ctcgttgtat caggttgaaa aataactatg tcaaaacgaa cattcgcggt
      601 gatattaacc ttgttgtgta gcttctgtat cggccaggcg cttgcagggg gtatcgtttt
      661 acagcgaacg cgagtgatct atgatgccag ccgcaaagag gctgcgttac ctgtcgcaaa
      721 caaaggcgca gaaacgcctt atttactgca atcatgggta gataatatag atggtaaaag
      781 ccgtgcccca tttattataa ccccaccgct atttcgtctt gaggctagcg atgactcatc
      841 actgcgaatt attaaaacag ctgataacct gcctgaaaat aaagagtcgc tgttctacat
      901 taatgttcgt gccattccag caaagaaaaa atcagatgat gttaatgcta acgagttgac
      961 gctggtattt aaaacgcggg tcaaaatgtt ttatcgcccc gcacacctga agggacgggt
     1021 aaacgatgcg tggaaatcac tggaatttaa acgtagtgac cattcactca atatatataa
     1081 cccaactgaa tattacgtcg tatttgccgg actggcagtc gataaaaccg atctcacaag
     1141 caaaattgaa tatatcgcgc ccggagaaca taaacagtta ccacttccag catctggcgg
     1201 aaagaacgtg aaatgggctg cgatcaacga ttatggcggc agttccggga cagaaactcg
     1261 tccactgcaa taaaaaatat aaaaacacag gccatcaggg aatgccacaa cgacaccacc
     1321 agggacataa acgcacaccg aaacagttgg cgctcattat caaacgttgt ttgccgatgg
     1381 tgctcactgg cagcggcatg ctttgcacta ccgctaacgc cgaagagtat tatttcgacc
     1441 ccattatgct ggaaactaca aaaagtggta tgcaaacaac cgatctgtca cgtttttcaa
     1501 aaaaatacgc acaactacca ggaacttatc aggttgatat ctggctgaat aaaaagaagg
     1561 tttcacagaa aaaaattaca tttaccgcca atgcagagca acttctgcag ccacagttta
     1621 cggtagaaca actacgtgag ctgggtatta aggtggatga aatcccggcg ctggctgaaa
     1681 aagatgacga tagcgtgatc aactcgcttg aacaaatcat tcccagtaca gctgctgaat
     1741 ttgatttcaa tcatcagcga cttaatttga gcattcccca aattgcactg taccgtgatg
     1801 caagaggtta cgtctcccct tctcgttggg acgatggtat accaacgctg tttaccaact
     1861 actcgtttac aggttctgat aaccgttacc gccagggcaa tcgtagccaa cgacagtacc
     1921 tgaatatgca aaatggtgct aattttggcc cctggcgatt acgtaactat tctacgtgga
     1981 cacgcaacga tcaggcgtca agctggaaca ctatcagtag ttatttacaa cgtgatatca
     2041 aggcgttgaa gtctcagttg cttctgggag aaagcgccac cagcggcagt attttttcca
     2101 gctacgcctt tactggcgtg caactcgctt ccgacgataa tatgttgcca aacagccagc
     2161 gcggatttgc cccaacggta cgcggtatcg caaacagtag tgcaatcgtg actatcaggc
     2221 aaaatggtta tgtgatctat caaagcaacg tgccagcggg tgcctttgaa attaacgatc
     2281 tctacccctc ttccaacagc ggcgatttag aagtcacgat tgaagaaagt gacggtacac
     2341 aacgtcgctt tatccagcct tattcttcat tacccatgat gcagcgacct gggcatctaa
     2401 aatatagcgc gaccgctgga cgctatcgcg ctgatgcaaa cagtgatagc aaggaacccg
     2461 aatttgctga agccacggca atatatggtt tgaataatac ttttacgctg tatggcggcc
     2521 tgctcggttc tgaagattat tatgcgctgg ggatcggtat cggcggcaca cttggcgcac
     2581 tgggcgcgtt gtcgatggat atcaacagag ctgacaccca attcgataac cagcactctt
     2641 ttcatggcta tcaatggcgt actcagtaca tcaaagatat cccggaaacc aacaccaata
     2701 tcgctgttag ctactatcgc tataccaacg atggctattt tagttttgat gaagccaata
     2761 cccgcaattg gggctataac agtcgccaaa aaagtgaaat tcaattcaac atcagccaga
     2821 caatatttga tggggtaagt ctgtatgcct ccggttcaca gcaagactat tggggcaata
     2881 acgagaaaaa cagtaatatc tctgttgggg tttccggcca gcaatgggga attggttaca
     2941 gcctgaatta tcaatacagc cgctacactg atgaaaataa tgaccgtgca ctctctttga
     3001 atctcagtat tccgttagaa cgctggtgga tttgccccta tatttccaga catctgttat
     3061 cacttaacc
//
LOCUS       DAEBXN010000255         2968 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.258, whole genome shotgun sequence.
ACCESSION   DAEBXN010000255 DAEBXN010000000
VERSION     DAEBXN010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.258"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(<1..924)
                     /locus_tag="KJL99_004387"
     CDS             complement(<1..924)
                     /locus_tag="KJL99_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HBD4438860.1"
                     /translation="MKAIDNIFKYIPSNHKDKHSDKVNNHQHHSKVDKTHRSKIVEID
                     KLDNDSQIDNDFGMHIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKVNGLSIIPENF
                     FTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNANTAYFGD
                     TDGRVGAVLYSLLVSGHIGIREKGWSLLCELLKHEDMASSAYIHKNNKLLYNLLNTRD
                     MILNELHQHVFLKDDAITPCIFLGDHTGDRFS"
     gene            1309..2340
                     /locus_tag="KJL99_004388"
                     /pseudo
     CDS             1309..2340
                     /locus_tag="KJL99_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
     gene            complement(2427..2903)
                     /locus_tag="KJL99_004389"
     CDS             complement(2427..2903)
                     /locus_tag="KJL99_004389"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438861.1"
                     /translation="MTNKKMYFILILVFTLLQVCFFALWKARDGSTTSLECTSTLTRN
                     AKTDHSLYYSANLSVILKKDGSGSFTIVGLTDEDTPRKFSHSYFFTYKIDSNGRISGN
                     AKAKVSGLENQIKDENFRLNFLDASLTGKGNARLSKFNNVYIFSIPGLIINTCAPI"
     gene            complement(2896..>2968)
                     /locus_tag="KJL99_004390"
     CDS             complement(2896..>2968)
                     /locus_tag="KJL99_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBD4438862.1"
                     /translation="LSRCIKSSNDETYAICSEVLLYD"
BASE COUNT          874 a          578 c          539 g          977 t
ORIGIN      
        1 actaaatcta tcgcctgtat gatccccaag gaagatacaa ggggttatcg catcgtcctt
       61 taaaaaaaca tgttgatgca actcattcaa tatcatgtct ctcgtattta ataaattgta
      121 gagcaattta ttatttttat gtatgtatgc agatgaagcc atgtcttcgt gttttaataa
      181 ttcacataat aaactccaac ccttttccct gatgcctata tgccctgaaa cgagcaatga
      241 ataaagtaca gcgcctactc ttccatctgt atcgccaaaa taagccgtgt tggcattttg
      301 tgggttaatc acaccacatt gtttataaaa gtctatttta gaaaacacag atttaacata
      361 gctatcaaac ttatccttag gaaacgtctc tttagagaca tcccaacgcc agtcagcttt
      421 aaaattaccg ctaagaagag taaagaaatt ttcaggtatg atagaaagcc cattaacttt
      481 ataaattatg tctgatttat ctccggagaa attaataaaa taataaccat ccttacactc
      541 aatcttaact gtaatgggag acgataattt tatcacatgg ttattgtctg gtataattgt
      601 aaagataaac gtgttagtag tattatcggc aaaactatta tattcttgaa tatctataga
      661 tggctcacta ttgtaaaacc atattttctc catctgaaga ctatgttcat ttaccctcca
      721 gtgaccatgt tgcaggaaat agatgatatg catgccaaaa tcgttatcta tttgcgaatc
      781 attatccagc ttatcaattt caactatctt tgaacgatgt gttttatcaa ccttgctatg
      841 gtgttgatga ttattaactt tatctgaatg cttatcttta tggtttgaag ggatgtactt
      901 aaatatattg tcaattgctt tcataaataa cccgcgatat ttttgacctg ttagtaaaaa
      961 ataaatgata catgtctgaa agacatattt acgacgatat aaaacaataa tacttatatg
     1021 ttcgctgatt ttaagcaccg agactatccg gtcttttact aaataatgga aaacaatcca
     1081 tccctggaag acaattgtaa aaaaaaacaa cagaataaga attaatctta ggataaatat
     1141 ttatttatca tggccttttg accgctaaaa aatggcactc ggcacagtaa accccgctat
     1201 agttatgcta tgcatttgtc tttaatgata tctcgtcgtt atattaacag gctgaaatta
     1261 tcattaatgc acatttcaat attagcaggg atacctgagg agtttaaagt gaacatatat
     1321 atcgggtagc ttttcaaatt aatccctttg attataggct taatttgcat cgcgttaggt
     1381 ggctttgtgc tggaaagttc agggcaacgt cacgggtcat gtgctgattt ctctggcggc
     1441 catatgcctg gcattattca ctaccgcatt tattatcatt tcgcaactca cgcgcggcgt
     1501 taatacgttt tacaatacat tgttccccat tattggctat gcggggtcaa ttatcaccat
     1561 gatatggggt tgggcactgt tagcaggtaa tgatgtgatg gcagacgagt ttgttgccgg
     1621 ccatgttatt tttggcgttg gtatgattgc cgcatgtgta tcgacggtgg cagcgtcgtc
     1681 cggtcacttt ctgctcattc ccaaaaatgc agcggggagc aagagcgacg gaacaccggt
     1741 acaggcttat tcttcattaa tcggtaattg cctcattgcc gttcccgttt tactcaccct
     1801 gctcggtttc atttggtcta ttacgctgtt acatagtgcc gacataactc cgcattatgt
     1861 cgccttgggt taaccgcaat ctgtgcctgt ctaattggcc tagtggccac aatcgtccat
     1921 caaacacgta atacgttctc atccaaagag cactggctgt ggtgttattg ggttattttt
     1981 ctcggctcag tcacggtgct gcaggggata tacgtcttag tcagttccga tgcaagcgcc
     2041 cgactggctc ccggcattat tctcatttgc ctcggaatga tctgttacag catattctca
     2101 aaagtctggc tactggcact ggtctggaga cgtacctgtt cgttagccaa cagaataccg
     2161 atgataccgg tatttacctg tctgttttgc ttgtttttgt catcattcct cgcggaaatc
     2221 gcacaaactg agctggacta ctttattcca tctcgggttc tggttggatt aggcgcagta
     2281 tgttttacat tgttctctat tgtttcaata ttagaggccg gttctgcgaa aaaaaattaa
     2341 aatttacata atcccagcgt tattcatatc ttaacgctgg ttttttacga acttagtgaa
     2401 ataatgtaga atataaaatt ataattttat attggagcac atgtgttaat gatcaacccc
     2461 gggatactaa aaatatagac attattaaac ttgcttagcc tcgcattgcc tttacctgtt
     2521 aaagatgcat cgagaaaatt gagtctgaag ttctcatcct ttatctgatt ttccagtccc
     2581 gaaactttgg ccttagcatt acctgagatc cgtccgttac tatctatttt gtaggtaaaa
     2641 aaatacgaat gggaaaattt cctcggtgta tcttcatcag ttaagccaac aatcgtaaaa
     2701 ctaccgctac catctttttt taaaatgact gaaaggttag cagagtagta taaagaatga
     2761 tctgttttag catttcttgt taacgtcgac gtacattcaa gagacgttgt gctaccatca
     2821 cgagccttcc agagagcaaa aaaacacacc tgtagcaatg tgaaaacaag tattaatata
     2881 aaatacattt tcttattagt catataatag cacctctgaa caaattgcat aagtttcgtc
     2941 attagaggat tttatacatc ttgataaa
//
LOCUS       DAEBXN010000256         2951 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.259, whole genome shotgun sequence.
ACCESSION   DAEBXN010000256 DAEBXN010000000
VERSION     DAEBXN010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.259"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            141..2009
                     /gene="kup"
                     /locus_tag="KJL99_004391"
     CDS             141..2009
                     /gene="kup"
                     /locus_tag="KJL99_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HBD4438863.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGGEVLYADMGHFGKFSIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2176..2595
                     /gene="rbsD"
                     /locus_tag="KJL99_004392"
     CDS             2176..2595
                     /gene="rbsD"
                     /locus_tag="KJL99_004392"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HBD4438864.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2603..>2951
                     /locus_tag="KJL99_004393"
     CDS             2603..>2951
                     /locus_tag="KJL99_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBD4438865.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWK"
BASE COUNT          658 a          701 c          779 g          813 t
ORIGIN      
        1 aagggggggc tttttctttg agccaggtta atgtggccgc atttaggagt acgattttgc
       61 cgttaatcgt gcatactgtg cgcttttttg tgggccaagg gactaagcac acatttcata
      121 tttcaacgaa agactagtct atgagcactg ataataagca atcattgccc gcgattaccc
      181 tcgcggcgat tggagttgtc tacggcgata ttggtaccag cccgttatat acacttcgtg
      241 aatgtttgtc cggccagttt ggttttggcg ttgaacgcga tgccgtgttt ggctttttat
      301 cgctgatctt ctggctgcta atctttgtgg tttccattaa atatctcacc ttcgtgatgc
      361 gggcagataa cgccggtgag ggggggatcc tgacgttgat gtcgcttgcc gggcgtaata
      421 cgtcggcgcg aaccacatca atgctggtga ttatggggct aatcggcggc agctttttct
      481 atggtgaagt cgtcataaca cccgctattt cggtgatgtc agccattgaa ggtctggaaa
      541 tcgtcgcccc gcagctggat acctggatag ttcccctctc aattatcgtt ctcactttat
      601 tatttatgat tcaaaagcat ggcacggcta tggtcggtaa gctgtttgcg ccgatcatgc
      661 tgacctggtt tttgattctg gcaggactgg ggttacgtag cattattgct aacccggaag
      721 tgctgcatgc gctgaatccg atgtgggcgg tgcatttctt ccttgaatac aaaacggttt
      781 cttttattgc attaggggca gtggtgctgt cgattacggg gggcgaggtg ctgtatgctg
      841 atatggggca ctttggtaag ttctctattc gcctggcgtg gttcaccgtc gtattacctt
      901 ccttgaccct taattacttc ggtcagggag cgttgttgtt aaagaacccg gaagcgatta
      961 agaacccgtt cttcctgttg gcaccggact gggcgctgat cccactgctg atcatcgccg
     1021 cactggcgac ggtaattgcc tcgcaggcgg ttatctctgg cgtcttctca ttgacgcgtc
     1081 aggcggtacg tctgggatat ttgtcgccga tgcgcattat tcacacctcc gaaatggagt
     1141 cagggcaaat ctatattccg tttgtgaact ggatgctcta tgtcgcggtc gtgattgtga
     1201 ttgtcagctt tgagcactcc agcaacctgg cggcggcgta cgggattgcg gtgaccggaa
     1261 ccatggtact gacgtctatt ctctcgacta ccgtggcacg tcagaactgg cactggaata
     1321 agtattttgt tgcgctgatc ctgattgctt tcctttgtgt cgatattcca ttgttcaccg
     1381 ctaacctcga taaactgctc tccggcggct ggttgccatt gagcctcggt actgtgatgt
     1441 ttatcgtgat gaccacctgg aagagcgagc gtttccgctt gctgcggcgg atgcatgaac
     1501 atggtaactc tctggaagcg atgattgctt cgctggagaa atcaccgcct gttcgcgtgc
     1561 ccgggaccgc ggtgtatatg tcgcgtgcaa tcaacgtcat tccctttgcg ctgatgcata
     1621 accttaaaca taacaaggta ttgcatgagc gggtgattct gttaactctg cgcaccgaag
     1681 acgcgccata tgtccataac gtccgtcggg tacagattga acaactgtcg cccactttct
     1741 ggcgcgtggt ggcaagttat ggttggcgag aaacgccaaa cgtggaagaa gttttccacc
     1801 gctgcggtct ggaaggatta agttgccgga tgatggaaac ctccttcttt atgtcgcatg
     1861 agtcgttgat cctcggcaaa cgcccgtggt atttgcgtct gcgcggcaag ctgtacttgc
     1921 tgctgcaacg taatgcgctg cgtgcaccag atcaatttga aatcccgcca aacagggtta
     1981 tcgaactggg tactcaggtc gaaatctaac gccagacgcc tcctttcttc ataagggggc
     2041 gtttttgttt tcatggttaa tcaccatgta aaacgtttcg aggttgatca catttccgta
     2101 acgtcacgat ggttttccca actcagtcag gattaagctg cggatcagcg aaacgtttcg
     2161 ctgatggaga aaaaaatgaa aaaaggcacc gttcttaatt ctgatatttc atcggtgatc
     2221 tcccgtctgg gacataccga tacgctggtg gtgtgtgatg ctggtttacc catccccaaa
     2281 agtacaacgc gtatcgatat ggcattaacc caaggtgtac cttcttttat gcaggtgctg
     2341 ggcgtcgtca caaatgaaat gcaggtcgag gctgccatta tcgcggaaga gatcaaacaa
     2401 cataatccgc aactccacga aacgttgctc actcaccttg agcagctgca aaaacaccag
     2461 ggaaatacca ttgaaattcg ttacaccacg catgaacaat tcaaacaaca aaccgcagaa
     2521 agtcaggcgg taattcgcag cggagaatgt tctccgtatg cgaatatcat tctctgtgct
     2581 ggcgtgacgt tctgaggccg tcatggaagc attacttcag cttaaaggca tcgataaagc
     2641 cttcccgggc gtaaaagccc tctcgggcgc agcgttaaat gtctatccgg gccgtgtgat
     2701 ggcgctggtg ggcgaaaacg gcgcgggtaa atccaccatg atgaaagtgc ttactggtat
     2761 ctatactcgc gatgccggca cgcttttatg gctggggaaa gaaacgacat ttaccgggcc
     2821 gaaatcttcc caggaagccg ggattgggat tatccatcag gaactgaacc tgatcccgca
     2881 gttgaccatt gccgaaaaca ttttcctcgg tcgtgagttt gttaatcgct ttggcaaaat
     2941 tgactggaaa a
//
LOCUS       DAEBXN010000257         2933 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.260, whole genome shotgun sequence.
ACCESSION   DAEBXN010000257 DAEBXN010000000
VERSION     DAEBXN010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.260"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            75..650
                     /gene="ssuE"
                     /locus_tag="KJL99_004394"
     CDS             75..650
                     /gene="ssuE"
                     /locus_tag="KJL99_004394"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HBD4438866.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQLIDYHHRPQFTPNLQTH
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            643..1602
                     /gene="ssuA"
                     /locus_tag="KJL99_004395"
     CDS             643..1602
                     /gene="ssuA"
                     /locus_tag="KJL99_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HBD4438867.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1599..2744
                     /gene="ssuD"
                     /locus_tag="KJL99_004396"
     CDS             1599..2744
                     /gene="ssuD"
                     /locus_tag="KJL99_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HBD4438868.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAY
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            2753..>2933
                     /locus_tag="KJL99_004397"
     CDS             2753..>2933
                     /locus_tag="KJL99_004397"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter"
                     /protein_id="HBD4438869.1"
                     /translation="MMATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPE
                     GVVTAFWTLSASGELW"
BASE COUNT          660 a          801 c          805 g          667 t
ORIGIN      
        1 tgtctctccg acagactgac cgaattgtta tcaatgttaa caaaaaaaag aacaattggt
       61 tataaggaga gagtatgcgt gtcatcaccc tggcgggtag tcctcgcttt ccttctcgct
      121 ccagctcctt gctggaatat gcgcgggaaa aactaaatgg cctagatgta gaggtttatc
      181 actggaatct gcaaaacttc gccccggaag atctgcttta tgctcgtttc gatagtccgg
      241 cactcaagac cttcaccgaa cagctgcaac aggctgatgg gctgattgtc gccacgcctg
      301 tgtataaagc cgcctattcc ggcgcgctga aaaccctgct cgacctgctg ccagagcgcg
      361 ctttgcaagg caaagtggtg ctaccgctgg cgacgggcgg taccgtggcc catctgctgg
      421 cggtcgatta tgcccttaaa ccagttttaa gcgcactgaa agctcaggag atcctgcacg
      481 gcgtgtttgc cgatgactca caattaattg attaccatca cagaccccag ttcacgccaa
      541 atctgcaaac ccatcttgat accgcgctag aaactttctg gcaggcattg caccgccgcg
      601 atgttcaggt tcctgacctt ctgtctctgc gaggtaatgc ccatgcgtaa catcattaaa
      661 ctggcgctgg cgggattgct tagcgtctct acttttgcgg ttgctgcaga atcctcgcct
      721 gaagcgttac gtataggcta tcagaaaggc agtattggta tggtgctggc aaaaagccac
      781 cagttactgg aaaaacgcta tccgcaatca aaaatctcct gggtggagtt ccccgcgggt
      841 ccgcaaatgt tggaagcgtt aaacgttggc agtattgatc tcggcagtac cggggatatt
      901 ccgccaatct ttgcccaggc tgccggggct gatttggtat atgtgggcgt cgagccaccg
      961 aagcccaaag ccgaagtgat tctggtggca gaaaacagcc cgatcaaaac cgtagccgat
     1021 cttaaaggtc acaaagttgc ctttcagaaa ggttccagtt cacacaacct tttactgcgt
     1081 gcactgcgtc aggccggact taaattcacc gatatccagc ccacttacct gacgcctgcc
     1141 gatgcccgcg ccgcgttcca gcaaggtaac gttgacgcct gggctatctg ggatccctac
     1201 tactccgctg cgttattaca gggcggcgtg cgggtgctga aagacggcac cgatctcaat
     1261 caaactggat cgttttatct ggcagctcgt ccctatgcag aaaaaaacgg cgcttttatt
     1321 cagggcgtac tggcaacctt tagtgaggcc gatgcgttaa cccgcagcca gcgcgaacaa
     1381 agcatcgctt tactggcaaa aacgatgggc ttaccggcac cggtgattgc ctcttactta
     1441 gatcatcgcc ctcctaccac catcaaaccg gttaacgccg aggttgccgc cttacagcag
     1501 caaacggcag atctgtttta tgaaaaccgt ctggtgccga aaaaagtcga tattcgccag
     1561 cgcatctggc agcccactca actggaagga aaacaattat gagtctgaat atgttctggt
     1621 ttttaccgac ccacggtgac gggcactatc tgggaacgga agaaggttca cgcccggttg
     1681 atcacggtta tctgcaacaa attgcgcaag cggcggatcg tctgggctat accggtgtgc
     1741 tgatcccaac ggggcgatcg tgtgaagatg cgtggctggt tgcagcgtcg atgatcccgg
     1801 tgacgcagcg gctgaagttt cttgtcgccc tgcgtcccag cgtaacctca cctactgttg
     1861 ccgcccgcca ggccgccacg cttgaccgtc tctcaaatgg tcgtgcgttg tttaatctgg
     1921 tcacaggcag cgatccacaa gagctggcag gcgacggcgt gttccttgat catagcgagc
     1981 gctacgaagc ctcggcggaa tttacccagg tctggcggcg cttattgctg ggggaaaccg
     2041 tcaatttcaa cggtaaacat attcatgtgc gcggagcaaa actgctcttc ccgccgattc
     2101 aacagcctta tcctccgctt tactttggcg gatcgtcaga tgtcgcccag gagctggcgg
     2161 cagaacaagt tgatctctac ctcacctggg gcgaaccgcc ggaacttgtt aaagagaaaa
     2221 tcgaacaagt gcgggcgaaa gctgccgcgt atgggcgcaa aattcgtttc ggtattcgtc
     2281 tgcatgtgat tgttcgtgaa actaacgacg aagcgtggca ggccgccgag cggttaatct
     2341 cacatcttga tgatgagact atcgccaaag cacaagccgc attcgcccgg acggattccg
     2401 taggacaaca gcgaatggcg gcgctacata acggcaagcg cgacaatctg gagatcagcc
     2461 ccaatttatg ggcgggcgtt ggcttagtgc gcggcggtgc cggaacggcg ctggtgggcg
     2521 atggtcctac ggtcgccgcg cgaatcaacg aatacgccgc gcttggcatc gacagctttg
     2581 tgctttcggg ctacccgcat ctggaagaag cgtatcgggt tggcgagttg ctgttcccac
     2641 atctggatgt cgccattccg gaaattcccc agccgcagcc gctgaatccg caaggcgaag
     2701 cggtggcaaa tgattttatc ccccgtaaag tcgcgcaaag ctaaggaaca cgatgatggc
     2761 aacgccagtg aagaagtggt tattgcgcgt tgccccctgg tttttaccgg tgggcatcgt
     2821 ggcggtgtgg caactggcct cctcggttgg ctggctgtcg acgcgtattt tgccttcacc
     2881 ggaaggagtg gtgacggcgt tctggacgct ctccgccagc ggcgaactgt ggc
//
LOCUS       DAEBXN010000258         2925 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.261, whole genome shotgun sequence.
ACCESSION   DAEBXN010000258 DAEBXN010000000
VERSION     DAEBXN010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.261"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(<1..460)
                     /gene="intS"
                     /locus_tag="KJL99_004398"
     CDS             complement(<1..460)
                     /gene="intS"
                     /locus_tag="KJL99_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HBD4438870.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISAGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGA"
     gene            complement(622..696)
                     /locus_tag="KJL99_004399"
     tRNA            complement(622..696)
                     /locus_tag="KJL99_004399"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(661..663),aa:Arg,seq:cct)
     gene            complement(770..1702)
                     /locus_tag="KJL99_004400"
                     /pseudo
     CDS             complement(770..1702)
                     /locus_tag="KJL99_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
     gene            1996..2751
                     /gene="mlaA"
                     /locus_tag="KJL99_004401"
     CDS             1996..2751
                     /gene="mlaA"
                     /locus_tag="KJL99_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HBD4438871.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADALYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
BASE COUNT          713 a          708 c          700 g          804 t
ORIGIN      
        1 ttgcaccgcg atcttcaaat ctgcggatta cttccagcag ttgcatcggc tcaatatcct
       61 gaatctcaag gccgccgatg atgggtaaaa tgtcgtcatc aaacattttt gcaagttcag
      121 tcgcataccc tactgaccag acttgcttct tgtgttcgta ccattccttg taaatcgcac
      181 taaatgaatt gttgttagac gaagcctttt tcgcctttac cggatcgatg ccagccgaga
      241 tgtctttcct cgcagtccat gctttatccc ttgcctcctg caaagtcata agcggatatt
      301 ttccgacggt caggattttc tccttaccgt caatcttgta gcgaagctgc catacctttt
      361 tcccggatac agggacataa aggtacaggc cattaccatc gagaaggcgg tatggttttt
      421 ctttcggctt tgctgcttca atctgcttaa cggtgagcat gggtaaaaat ccggtgggta
      481 aaattatttt atccactttt tacccgtcat ggagtgcggc tgtcaacgat ctgacgcgaa
      541 ccatgacgaa ctgtgaatat acggaaggct tgatattcag gggattttgc ggactggtac
      601 ggatgggagc gaactgataa atggtgtccc ctgcaggaat cgaacctgca attagccctt
      661 aggaggggct cgttatatcc atttaactaa gaggacaatg cggcgagtat acccgctaat
      721 ggactgcggg gtaagtacgc tgccgctcga ttgcttaaac cctcgccatt tatgctgggt
      781 ttttatcatt tttcttaatg ttttccgcac gttctgcttt ttggcgtgct tctgctttac
      841 gcttgttgct catgtcgtta cgaatctgtg catgactcat taacgcgaag ataaaggtgc
      901 cgccgcagat gttccccgct aaagtaggta gtgcgaaggg ccagatgaaa tcgctccagt
      961 gcagcgtacc gttaaacacc agatagagga tttcaacaga accgaccacg atatgggtgg
     1021 tgtcacccag ggcaataagc caggtcatca atataatcac cacaatcttt gccgcacctg
     1081 ctgcaggaaa catccatacc atagtggcga tcagccagcc ggaaatgatc gcgttggcaa
     1141 acatctcgct gggggtgttc ttcatcacat ccatgccgat tttgacaaat gcatcgcgag
     1201 tttcttcatt gaagataggc atatattcaa atgcccacgc agcaatacca gtcccgagaa
     1261 tattacccag cagcacgact ccccataacc gcataagtaa gccaacgttg ctcattgtcg
     1321 gtttttgcat gacgggtagt accgcagtca cggtgttttc ggtaaataat tgctggcggg
     1381 ccattatgac gataataaaa ccaaaggtat aaccgagatt ctccagcaag aagctgcccg
     1441 gcacaccttc cagttcggca tgaaatatcc cttttgccag taacgaagcg cccatcgaca
     1501 gacccgccgc aatggctgac cacagtagcg ccattgcgtc gcgttctagc tctttttcac
     1561 catcctggcg gatatgctca tgaattgcca tcgctcggga ggggagtcgg tcttcatcta
     1621 tttctatttt tttgccgcgc tctttttctt cgctctaaac ttcaatttcg tcgctgtgtt
     1681 gatcaatttt gtcgttgtcc atggtctctt ctcttgaatt agcacgtata gctaaagcgt
     1741 agcggctttt ttgctcgcaa ctggcgggag ttactctgaa aatgtagaaa aggctgcgtt
     1801 tgcctttttc tgtttctata gaatcaagta gcctacaggg cggcgattac caggctatga
     1861 tcaaatcagc aaatcagggc gtctggacat cagttgacgt gctgttacaa tcgcccacac
     1921 ctaaacaggc ggatacggta tcgttccgtc atggatggca aactgcataa gccataaaaa
     1981 aacagggaga catttatgaa gcttcgcctg tcggcgcttg ctctgggaac tacgcttctg
     2041 gtggggtgtg cgagttccgg tacagatcag caagggcgtt ctgacccgtt agaagggttc
     2101 aaccgcacca tgtacaactt caacttcaac gtattagacc cgtatattgt tcgaccggtc
     2161 gctgtcgcct ggcgtgatta tgttccgcaa ccggcgcgta atggtttgag caactttact
     2221 ggcaaccttg aagaacctgc ggtgatggtt aactacttct tgcagggcga cccttatcag
     2281 gggatggtcc actttacccg ctttttcctg aacaccattt tggggatggg cggctttatt
     2341 gatgttgcag ggatggcgaa cccgaaactg caacggactg aacctcaccg cttcggtagt
     2401 acgcttggtc attatggcgt gggttatggg ccttacgttc agttaccgtt ctacggtagc
     2461 ttcacgctgc gtgatgacgg tggtgatatg gcggatgctc tttacccggt tctttcctgg
     2521 ctgacctggc cgatgtctgt gggtaaatgg acgcttgaag ggatcgaaac ccgcgctcag
     2581 ctgctggatt ccgatggtct gctgcgtcag tcgtccgatc cttatattat ggtgcgcgaa
     2641 gcgtacttcc agcgtcatga tttcatcgct aatggcggcg aactcaaacc gcaggaaaat
     2701 ccgaacgcgc aagcgattca ggatgattta aaagatatcg attctgaata agaaacaaat
     2761 aaaaaaggtg agtcgcaaaa ctcacctttt ttgttgttat ccctcaatta ccacaagtaa
     2821 aaggagataa accgacaatg acccggacgt aatgatgtaa aaatactgat gtttgtcact
     2881 gactatttcc gatattcagg cgttgaaaaa tcaaaaaact ggaaa
//
LOCUS       DAEBXN010000259         2910 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.262, whole genome shotgun sequence.
ACCESSION   DAEBXN010000259 DAEBXN010000000
VERSION     DAEBXN010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2910
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.262"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            625..1224
                     /locus_tag="KJL99_004402"
     CDS             625..1224
                     /locus_tag="KJL99_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HBD4438872.1"
                     /translation="MQTYNTNPAYEMAPQLLEHFNQHLDSLFGVYSKLLPFRMDFAYR
                     KNTLSYRCACRYAMCAEMLQLINEVGEKLVGYAWVMEYTERKGLHIHFVGYLNGQSHR
                     SSYLVSRLMGDIWRRVTEGNGYYHWCRFNKNYPVNINHVIHYSDHKAVNDLRYAISYL
                     AKREQKECGIILKCSGLPEKSNRGRPRLDSPLPGICALV"
     gene            1597..1980
                     /locus_tag="KJL99_004403"
     CDS             1597..1980
                     /locus_tag="KJL99_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438873.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYDSYTCHLCGSALRYHPQY
                     DTERPWFEHADEGLTEHGHECPYVRPERREIRLIKRLQQFVPDTLPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGRFSEEGGAE"
     gene            1977..2402
                     /locus_tag="KJL99_004404"
     CDS             1977..2402
                     /locus_tag="KJL99_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438874.1"
                     /translation="MNGIKTGLGITPGEHIISADSALSRNIRQCFCLSCHGRLILQTD
                     AQGAWFEHDLHALSEQQKADCVVLNPEKSHPYIEDMAMFLSPLPVVLEWHCVMCEQFF
                     HGKKFCEACGTGIYCRAVCTKSVYSYQPDLFEDCGGAST"
     gene            2685..>2910
                     /locus_tag="KJL99_004405"
     CDS             2685..>2910
                     /locus_tag="KJL99_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBD4438875.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQSEYDSQWATICSIAPKIGCTP
                     ETLRVWVRQHERDTGGGDGGLTTAERQRLKE"
BASE COUNT          724 a          705 c          745 g          736 t
ORIGIN      
        1 atggcttcac ccgtgttaat atcctggata actgtgtttg aaggatttct gttaataatc
       61 tgctcctgca actcacctga tgagtattag tggtaataca taatcttact cgtaaaagag
      121 tataccttat gtcattaaac agtcttccgg cgtgttgttc aatcctgtac ctctgttact
      181 tatctggtac gttgatatgt gtaatgctac gttaattgca atatctggtt atggctttat
      241 accttcagca tttaaaatat tttattaccg gagaaacagg gaagtggaaa gacttttggg
      301 ataatgtcag gccattctga cgttaatcac agtaccaaat aacgagaggg taatggaagt
      361 taaaacctgc gtaagttcct ctgttgttca ctggaaaaag tgattgccca cacgcgttat
      421 aaaggccttt ccgctgcagc ctggttaaca gagcttcagg cgttatatgc gctgtatggc
      481 tgttacgtat gctgttcttt gcctggttct gatacccgtg gaagtcgtta ccggaggaaa
      541 cacgttcacc ctactacctg agaacatctt cttcaaaaat tacggcaacg cttcttcctg
      601 tgttacagaa cacgaggaag aaacatgcaa acttataaca ccaatcccgc ttacgaaatg
      661 gctccccagc tcctggagca ctttaaccag catctggatt cattattcgg ggtctattcg
      721 aaactgttac cgttcagaat ggattttgct taccgaaaaa acaccctcag ctacagatgt
      781 gcctgcaggt acgccatgtg cgcggagatg ctgcaactga ttaacgaggt gggagagaaa
      841 ctggtcgggt atgcctgggt gatggaatat acagaacgta aagggcttca cattcatttt
      901 gtgggctatc tgaacggcca gagccacagg agttcgtatc tggtatccag attgatggga
      961 gatatctgga ggcgggttac cgagggtaat ggttactacc actggtgccg gtttaataaa
     1021 aattatccgg taaacattaa ccacgtcatt cactattcag atcacaaagc ggtgaatgat
     1081 ttacgctatg ccatcagcta cctggccaaa cgggaacaga aagaatgtgg catcatcctg
     1141 aaatgcagcg ggcttcctga aaaaagtaac cggggtcgac cacgactgga cagccccctg
     1201 ccggggatat gtgctctggt gtaaacccct gaacgacaag tacgggctgg cgctaatctt
     1261 tgctgtcggt tccggcggcc acttcagcgg cactgcgcat tttttacggc gtggtcattc
     1321 cgtatgaacc atacggagat ttaaccatga cacattcttt accattcctt ccggcaaccg
     1381 atacagcaca tcccccaatc tccatctctg cttactgact gttttttacg tcagtgggcc
     1441 attgtgtgac aaccacagtg ctgtaacagc aaccagcctg ctttacccaa actccagtcc
     1501 agattttaac tgaaccgcca ttcacaccct gaccacgctg cccgtaccgg gacggctgtt
     1561 gcctgcgtgt cgtctggcgg ctgaggaaca tatactatgt acgcaaaatc ttttctcgca
     1621 cttgatggca acggacgtct gaccggtgcc cgtaccgcac agactgcacc ttacgatagc
     1681 tacacctgcc acctttgcgg cagtgcactc agataccacc cgcaatacga cactgaacgg
     1741 ccctggtttg aacacgctga cgaagggctg acggagcacg gtcatgagtg cccttatgtc
     1801 aggccggaac gcagagaaat acggttgatt aaacgtctgc agcaattcgt cccggatacc
     1861 ttacccgtag tacgtaaagc cagctggcac tgcaggcaat gtcaccacga ttattatggg
     1921 gagcagtact gcacacactg ccagaccggg cgcttcagtg aggagggggg agcagaatga
     1981 acggtataaa aacggggctg gggatcacgc cgggtgagca cattatcagt gccgactctg
     2041 cgctcagcag aaatatccgg cagtgtttct gcctttcctg ccatggccgg ttgattctgc
     2101 agacagatgc tcagggggcg tggtttgagc atgatttaca tgccctgagt gagcaacaga
     2161 aggcggactg tgtggtgctg aatccggaga agtcccaccc ttacattgag gatatggcga
     2221 tgtttttatc gccattaccg gtcgtgctgg agtggcattg tgtgatgtgt gagcagtttt
     2281 ttcatgggaa gaagttctgt gaggcctgtg gtacggggat ttattgcaga gcggtatgta
     2341 cgaagtctgt ttacagctac cagccagact tgtttgagga ctgcggaggt gcgagtactt
     2401 aatccgaaat gcccgacctg ctcagggcgt tcagaggtat tgccaacctg tattttatct
     2461 ggtgtttcgc acccaccccg ttcgtgacac aaaggcgcga caccggatat ccgcagtcct
     2521 gcttacctga ataaaacccg gggtattgat tggtagtgtg gtgcggaatc gtaccgtgat
     2581 ctcgcattcg ccgaattcgc actgtggtgt caatcaacca tacgcgagag tgaaccgccc
     2641 cgggaatcct ggagactaaa cttcctgaga aagaggtaaa caggatgact aaaaatactc
     2701 gtttttcccc cgaagtccgt caacgggcag tccgtatggt tctggaaagt cagagcgaat
     2761 atgactcaca atgggcgaca atttgttcta ttgctccaaa gattggctgt acgccggaga
     2821 ctctgcgtgt ctgggttcgc cagcatgagc gggataccgg gggcggtgat ggagggctca
     2881 ccaccgctga acgtcagcgt ctgaaagagc
//
LOCUS       DAEBXN010000260         2899 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.263, whole genome shotgun sequence.
ACCESSION   DAEBXN010000260 DAEBXN010000000
VERSION     DAEBXN010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2899
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.263"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(<1..312)
                     /locus_tag="KJL99_004406"
     CDS             complement(<1..312)
                     /locus_tag="KJL99_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HBD4438876.1"
                     /translation="MISNDILRSVRYILKANNNDLVRILALDNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DI"
     gene            complement(359..1078)
                     /gene="yehT"
                     /locus_tag="KJL99_004407"
     CDS             complement(359..1078)
                     /gene="yehT"
                     /locus_tag="KJL99_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HBD4438877.1"
                     /translation="MIKVLIVDDEPLARENLRIFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(1075..2760)
                     /gene="btsS"
                     /locus_tag="KJL99_004408"
     CDS             complement(1075..2760)
                     /gene="btsS"
                     /locus_tag="KJL99_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HBD4438878.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
BASE COUNT          751 a          774 c          658 g          716 t
ORIGIN      
        1 aatgtcatcg gttttcagcg aaaacgcgat gcgtaatttt ttcagcacga tgttgttatt
       61 aatgcgacgt tccggctcca gtgccggagc agactcatcc ttgccgcgtt tttcataaat
      121 caggccattg aggaatgacg acaaaacaat gtccggacaa cgctgaaaac cctcttcgtc
      181 ttctttacgt agccagacgg cgatctgttc cgcggtggct tcgacattat ccagcgccag
      241 aatacgcacc aggtcattat tattggcttt caaaatgtag cgcacgctgc gcagaatatc
      301 gttacttatc atgaggcctt caggtgttga tgaggcaaaa agccatttta gcagtctttt
      361 acaggccaat cgcctctttt aagcttttca gataacggcg gctgaccggc acggttaagc
      421 cattacgcaa aatcaactcg gcctggccgt tatcttccag acgaatctcc tgtaaatgcg
      481 cgaggttaac cagatactga cgatggcagc gcagtagtgg tgtacgactt tccaaggtac
      541 gtaatgtcaa ttcggtaaag ccctctttcc cttcgtggct ggtaacgtag acaccgctca
      601 tccgactgct gacaaatgcc acatctttca tttgcagcaa ataaatccga ctatgccccg
      661 tacaagggat aaatttcagc gcctgttgat tttccggtaa cagcgaaaca tcctgcttgc
      721 tgcgctcctg acgcaatcgc gccagcgttt tctccagtcg cgcttcatca attggcttca
      781 gcagataatc aaaggcatgt tcttcaaagg ctttaatagc gtattcgtca aacgcagtga
      841 gaaaaacaat atacgggcga tgttccgggt caagcatccc caccatttcc agaccactga
      901 tgcgcggcat ctggatatcg agaaacagca catccgggcg cagtttatgc accgcgccga
      961 tcccttccac ggcgtttgaa cactctccaa cgatttcaat atcgctctgc tcctgcaaaa
     1021 atatacgcag gttctcccgt gctaacggtt catcatcgac aattaagact ttaatcatgc
     1081 ctcgtccctc catggtagtc gtaacgttat tcgggtgtaa ctatcaggct cacaggcgac
     1141 gcttattcca tagtcatcgc caaaccgttc acgtaaacgc ttatccacca gattcatccc
     1201 cagcccactg gcattggtta ccggttgata caaaccggca ttgtcttcga tctccagcat
     1261 caaatgttgc ccctcacgtc gggcgctgat tgccactcgc cctgtatcca gcagttgtga
     1321 tgtcccatgt ttaatggcgt tttccactat cggttgcagg gtaaacgcgg gcaattgctg
     1381 ctgggataat tcttgcggaa tagcaatgtt gacctgcaac cgcgactgga agcgcgcctt
     1441 ttcaatttgc agataagcgt tcacatgttc aatttcgtcg gcgagagtaa caaactccga
     1501 aggccgcttt aagtttttgc ggaaaaaagt ggaaagatac tgcaccagct ggctggcctg
     1561 ttcgctgtcg cggcggatca ccgctttaat ggtgttaagc gcattaaaca aaaaatgagg
     1621 attcacctgg gcgtgaagca gtttgatttc tgactgggtg agcatcgctt tttgccgctc
     1681 atattgccct gcaagaatct gcgccgaaag cagttgcgca atcccctcgc ccagcgtgcg
     1741 gtttattgaa ctgaataacc ggtttttggc ttcatacaat ttgatggtgc ccatcacgcg
     1801 ctgattttcg ccacgcaacg gaattaccag cgtcgacccc agtttgcatt gcggatgcaa
     1861 agagcaacga taaggtactt cgttgccatc agcgtagacc acttcaccgg tttcaatcgc
     1921 ttttaaggtg taagtcgaag aaatcggttt gccgggtaaa tggtggtcgt caccaattcc
     1981 ggtaaaggcc agcaatttct ctcgatcggt aatcgcgact gcaccaatat ccagctcctg
     2041 atacagtacc tgagccactt tcatgctgtt cacttcgtta aacccctgtc gcaaaatgcc
     2101 ttccgtcgag gctgccactt tcagcgcagt ggcagaaaaa gccgaagtgt atttttcaaa
     2161 catcgcgcgt ttatcgagca atatacgcat aaacagcgcc gcgccgacgg tattggtgac
     2221 catcattggc gcagcaatat tactcaccag acgcaccgca tcttcataag gtcgggcgat
     2281 cgcaaggatg atcagcattt gcaccatttc agcgacgaac gtgacggcac cggcggtaat
     2341 ggggttaaag actttatcag tgcgcccgcg acggatcagg atgctgtgta ccaggccgcc
     2401 gagcaatcct tcaacgatgg ttgagatcat gcaacttagc gcggtcatgc cccccatcga
     2461 atatcgatgt aagccgccgg tcagaccaac cagcccaccg acgaccggac cgccgagtaa
     2521 gccgcccatt accgcgccta tcgcacgggt attggcaata gaatcgtcaa tgtgcaaccc
     2581 aaaccaggtg cccatgatgc agaagatgga aaagacgatg tagcagagaa atttatgcgg
     2641 cagacgaacc gtgacctgca ttaacggtat gaataatggc gttttactca ttaaccacgc
     2701 aatgactaaa aaaacgcaca tctgctgaag cagcagcaac accagattaa aatcgtacat
     2761 acccgcaaac cacacttccc tttaaaacgc gtaacataca ttgcctgcgt ttaactttct
     2821 ttgaactctt gcagaaaaat gagaattcgt gagtacgatc actcaaaatc gcctggcaaa
     2881 aataaaatca ccctataga
//
LOCUS       DAEBXN010000261         2890 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.264, whole genome shotgun sequence.
ACCESSION   DAEBXN010000261 DAEBXN010000000
VERSION     DAEBXN010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.264"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..781
                     /gene="lsrF"
                     /locus_tag="KJL99_004409"
     CDS             <1..781
                     /gene="lsrF"
                     /locus_tag="KJL99_004409"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HBD4438879.1"
                     /translation="DWGMQSRLSWIFNPKTGKTVMLAFDHGYFQGPTIGLERIDINIA
                     PLFEHADVLMCTRGILRSVVPPATNKPVVLRASGANSILAELSNEAVALSMDDAVRLN
                     SCAVAAQVYIGSEYEHQSIKNIIQLVDAGMKVGMPTMAVTGVGKDMVRDQRYFSLATR
                     IAAEMGAQIIKTYYVEKGFERIVAGCPVPIVIAGGKKLPERETLEMCWQAIDQGASGV
                     DMGRNIFQSDHPVAMMKAVQAVVHHNETADRAYELYLSEKQ"
     gene            805..1095
                     /gene="lsrG"
                     /locus_tag="KJL99_004410"
     CDS             805..1095
                     /gene="lsrG"
                     /locus_tag="KJL99_004410"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HBD4438880.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDTVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            1135..1893
                     /gene="tam"
                     /locus_tag="KJL99_004411"
     CDS             1135..1893
                     /gene="tam"
                     /locus_tag="KJL99_004411"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HBD4438881.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(1897..2811)
                     /locus_tag="KJL99_004412"
     CDS             complement(1897..2811)
                     /locus_tag="KJL99_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HBD4438882.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTGDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
BASE COUNT          760 a          667 c          773 g          690 t
ORIGIN      
        1 ggattgggga atgcagtcac gcttatcgtg gatatttaat ccgaaaacgg gtaaaactgt
       61 gatgctggcc tttgaccatg gttattttca ggggccgact atcggacttg aacgcattga
      121 tataaatatc gccccgctgt ttgaacatgc cgatgtatta atgtgtacgc gcggcatttt
      181 gcgcagcgta gttcccccgg cgaccaataa gccggtggta ctgcgggcgt caggtgcgaa
      241 ctctattctg gcggaattaa gtaatgaagc cgtggcgtta tcgatggatg atgccgtgcg
      301 cctgaacagt tgcgcggtgg cggcgcaggt ttatatcggc agcgaatatg aacatcagtc
      361 gatcaaaaat attattcagc tggtcgatgc cggaatgaaa gtgggaatgc cgaccatggc
      421 cgtgactggc gtgggcaaag atatggtgcg cgatcagcgt tatttctcgc tcgcgactcg
      481 aatcgccgct gaaatggggg cgcaaattat caaaacctat tatgtcgaaa aaggttttga
      541 acggattgtt gccggatgtc cggtacccat tgttattgct ggcggtaaaa aattaccgga
      601 gcgcgagacg ctggaaatgt gctggcaggc tatcgatcag ggcgcttctg gtgtggatat
      661 ggggcgtaat attttccagt ctgaccatcc ggtggcgatg atgaaagccg tacaggcggt
      721 ggttcaccat aacgaaacgg ctgatcgggc atatgaactc tatctgagtg aaaaacagta
      781 actgcggatt taaggagaag aattatgcac gtcacactgg ttgaaattaa cgttcatgaa
      841 gacaaggttg acgagtttat cgaagttttt cgccagaacc acctgggctc tgtacaggaa
      901 gaaggtaatt tgcgcttcga tgtcttacag gacccggaag tgaattcgcg cttttatatt
      961 tacgaagcct ataaagatga agacacagtg gcattccata aaaccacgcc tcactacaaa
     1021 acctgtgtcg cgaaactgga atctttaatg accgggccgc gtaaaaaacg tctgttcaat
     1081 gggttgatgc cgtgaggcta caattggggt aacgcgctga cgggagtaaa aaaaatgtct
     1141 gactggaacc cctctttata tctgcacttt tccgctgaac gatcgcgtcc ggcggtggag
     1201 ctgcttgcca gagtgccgct ggagaatgtc gaatatgttg ccgatcttgg ctgtggccca
     1261 ggtaacagca ccgccctgct gcatcaacgt tggcctgcgg ccaggataac aggcatcgac
     1321 tcgtctccgg cgatgattgc tgaagcgcgc agtgctttgc cagactgcca gttcgtggaa
     1381 gcagacatcc gcaactggca accggaacag gcactcgatc tgatttttgc taatgcctca
     1441 ctgcaatggc tgcccgacca ctacgaattg tttcctcact tggtttcttt acttaatccg
     1501 cagggcgtgc tggcagtaca gatgccagat aactggctgg agccgaccca tgtgctcatg
     1561 cgcgaagttg cttgggaaca aaactacccg gatcgcgggc gtgagccgtt ggctggcgtt
     1621 catgcttact acgatatttt gagcgaagcc ggatgtgagg tcgatatctg gcgaaccact
     1681 tactatcacc agatgccgtc gcaccaggcg attatcgatt gggtgaccgc cactggatta
     1741 cgtccgtggt tacaggatct gaccgagagc gaacagcagc tttttcttac gcgctaccat
     1801 cagatgctgg aagagcagta tccactgcaa gagaacggac agatactgct ggcatttccg
     1861 cgtctgttta ttgttgcccg ccgtacggag taattatcac gtcagctggt aatgacgatc
     1921 gggaagaatt tttgctggaa tttctgcttc atcgttcatc tgtaacaggt caatttcaat
     1981 agcgttgcag atggcatcca gcggtaaatc attgttttca gtaccgaacg gatcttccag
     2041 ttcttccgcc agacaatcca gcgaaataaa agtgtaggaa atcagcacag agataaaagg
     2101 cgtcatgtaa tgcaggtcca cgaccagcgc gaacggcagc atgatacaaa acagataaac
     2161 ggtacgatgc aaaatcaggg tgtaggcaaa gggaattggc gtgtaggcaa tgcgctcaca
     2221 gcctgccaga accgctgaaa tatcattaag ccgatcgttg aggctaataa acaggatatc
     2281 tgaaagctgt ccattgcggc gctgaaccgc caaccattct cccattatta acaagatacg
     2341 gttagctgga gagttcgaag ccagtacacg ctgaagatct ccagtcttga gataatgagc
     2401 cagcacttcc gcctgtggct gtttgcgtaa tgtcatgcgt aaacagtggg cgaaggcgat
     2461 ttgcagcctg gcaaactccc ttacacttgc cgaatccggc aatgtcgttt ttacctctcg
     2521 cagtaacgac cgtgaggcaa tcatcaactg cccccaaagt tttcgcgctt caacgtaacg
     2581 ggcgtacccg gcattattac gaaaaccaag aaaaatggcg atggcgacac cgagaatgct
     2641 gaacggtgcg agggtgaatt tgatgcccag atgcgtgtac cagggcagca tgaatataac
     2701 agcaatagaa aagagaaaat tgagtagtaa gcgcgaggat atcttggata atactgagcc
     2761 gtgccagaca aaaatacggc gcagccagtg ttgttgtgga cgaacaatca tggttatctt
     2821 caggcgtgga aaagtcgccc tattaaacgt gattacgatc acattctcaa gacgctactt
     2881 acaaattacc
//
LOCUS       DAEBXN010000262         2852 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.265, whole genome shotgun sequence.
ACCESSION   DAEBXN010000262 DAEBXN010000000
VERSION     DAEBXN010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.265"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..203
                     /locus_tag="KJL99_004413"
     CDS             <1..203
                     /locus_tag="KJL99_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438883.1"
                     /translation="GASYRYSGAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSIAI
                     YLLAGCLISAMTALLMKDNQRS"
     gene            305..1759
                     /gene="amn"
                     /locus_tag="KJL99_004414"
     CDS             305..1759
                     /gene="amn"
                     /locus_tag="KJL99_004414"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HBD4438884.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITIGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSADLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDIWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            2102..2818
                     /locus_tag="KJL99_004415"
     CDS             2102..2818
                     /locus_tag="KJL99_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBD4438885.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPADLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
BASE COUNT          791 a          627 c          634 g          800 t
ORIGIN      
        1 ttggtgccag ttatcgctac agtggtgctg gagtcggtta tcaggttgcc agtgtggttg
       61 gcggtggatt tacacctttt attgccgctg cactcatcac ttactttgcc gggaactggc
      121 atagcatcgc catttatttg ctggctggat gcctgatttc tgcaatgacc gctttattga
      181 tgaaagacaa tcaacgctcg tgatagcctg gcgaagatca tccgatcttc gccttatact
      241 tttgtttcac attcctgtga catactatcg gatgtgcggt aattgtatgg aacaggagac
      301 acacatgaat aataagggct ccggtctgac cccagctcag gcactggata aactcgacgc
      361 gctgtatgag caatctgtag ttgctttacg caacgccatt ggcaaatata ttacaattgg
      421 cgaattacct gatgaaaacg cccgtaaaca aggtcttttt gtctatccat cactgaccgt
      481 aacctgggac ggtagcacaa caaatccccc caaaacgcgc gcatttggtc gttttactca
      541 cgcaggcagc tacaccacca cgattactcg ccctactctc tttcgttcgt atctgaatga
      601 gcaactaacg ttgctttatc aggattatgg tgcgcatatc tctgtgcaac cctcgcaaca
      661 tgaaatccct tatccttatg tcatcgatgg ctctgaattg acacttgatc gctcaatgag
      721 cgctgattta actcgctact tcccgacaac agaactggcg caaatcggcg atgaaactgc
      781 agacggcatt tatcatccaa cggaattctc cccgctatcg cattttgatg cgcgccgcgt
      841 cgatttttcc ctcgcacggt tgcgccatta taccggtacg ccagtagaac attttcaacc
      901 gttcgtcttg tttaccaact acacacgtta tgtggatgaa ttcgttcgtt ggggatgcag
      961 ccagatcctc gatcctgata gtccctacat tgccctttct tgtgctggcg ggaactggat
     1021 cactgccgaa accgaagcgc cagaagaagc catttccgac cttgcatgga aaaaacacca
     1081 gatgccagca tggcatttaa ttaccgctga tggtcagggt attactctgg tgaatattgg
     1141 cgtgggaccg tccaatgcta aaaccatctg cgatcatctg gcagtgctac gcccggatat
     1201 ctggttgatg attggtcact gtggcggatt acgtgaaagc caggccattg gagattatgt
     1261 ccttgcacac gcttatttac gcgatgacca cgttcttgat gcggttctgc cgcccgatat
     1321 acctattccg agcattgctg aagtgcaacg tgcgctttat gacgctacca agttagtcag
     1381 cggcaggcct ggtgaggaag tcaaacagcg gctgcgtaca ggtactgtgg taaccacaga
     1441 tgacaggaac tgggagttac gttactcagc ttctgcactt cgttttaact taagccgggc
     1501 cgtagcaatt gatatggaaa gtgcaaccat tgccgcgcaa ggatatcgtt tccgcgtgcc
     1561 atacgggaca ctactgtgtg tttcagataa accgttacat ggtgagatta agcttcccgg
     1621 ccaggctaac cgtttttatg aaggcgctat ttccgaacac ctgcaaattg gcattcgggc
     1681 gatcgatttg ctgcgcgcag aaggcgaccg actgcattca cgtaaattac gaacctttaa
     1741 tgagccgccg ttccgataat aagttatgaa ccttcttatc agaaatagat gtgaaggatt
     1801 aagtaagaca tctggcaaat agcctgcaat aaacgcgggc tgcttgcttc cttgggcaga
     1861 tgcgagtttt attatcgtct taatgatttc cacatattaa aagcaagtat gctttcaaaa
     1921 cacaattata aaaaatcccg ccaacaacat tagtttttat aaaattaaat ataagattat
     1981 ggctttagaa tatttttatt tctaatagac gagatttttc ctgttatgat ataatatgcc
     2041 gaattaacac atgttaacga tttaccagta atgtaaataa attttcgagg agatcattcc
     2101 agtgggacgt aaatgggcca atattgttgc taaaaaaacg gctaaagacg gtgcaacgtc
     2161 taaaatttat gcaaaattcg gtgtagaaat ctatgctgct gccaaacaag gtgaacccga
     2221 tccagaatta aacacatctt taaaattcgt tattgaacgt gcaaagcagg cacaagttcc
     2281 aaagcacgtt attgataaag caattgataa agccaaaggc ggcggagatg aaacgttcgt
     2341 acagggacgt tatgaaggct ttggtcctaa tggctcaatg attattgctg agacattgac
     2401 ttcaaatgtt aaccgtacga ttgctaacgt tcgcacaatt ttcaataaaa aaggcggcaa
     2461 tatcggagcg gcaggttctg tcagctatat gtttgacaat acgggtgtga ttgtatttaa
     2521 aggaacagac cctgaccata tttttgaaat tttgcttgaa gctgaagttg atgttcgtga
     2581 tgtaactgaa gaagaaggta atattgttat ttatactgaa cctgctgacc ttcataaagg
     2641 aatcgcggcg ctaaaagcag ctggaatcac tgagttctca acaacagaat tagaaatgat
     2701 tgctcaatct gaagttgagc tttccccaga agatttagaa atctttgaag ggcttgttga
     2761 tgcccttgaa gatgacgacg atgtacaaaa agtttatcat aacgtcgcaa atctctaatt
     2821 atattttaaa gaaatctgtc tttatggcag at
//
LOCUS       DAEBXN010000263         2851 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.266, whole genome shotgun sequence.
ACCESSION   DAEBXN010000263 DAEBXN010000000
VERSION     DAEBXN010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.266"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(<1..594)
                     /locus_tag="KJL99_004416"
     CDS             complement(<1..594)
                     /locus_tag="KJL99_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HBD4438886.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSS
                     ADLYFNLRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQN"
     gene            complement(661..1386)
                     /gene="fimC"
                     /locus_tag="KJL99_004417"
     CDS             complement(661..1386)
                     /gene="fimC"
                     /locus_tag="KJL99_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HBD4438887.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVINNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENMLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(1423..1962)
                     /locus_tag="KJL99_004418"
     CDS             complement(1423..1962)
                     /locus_tag="KJL99_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBD4438888.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIASNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(2027..2575)
                     /gene="fimA"
                     /locus_tag="KJL99_004419"
     CDS             complement(2027..2575)
                     /gene="fimA"
                     /locus_tag="KJL99_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HBD4438889.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTIHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
BASE COUNT          741 a          710 c          626 g          774 t
ORIGIN      
        1 attctgtaca ctatttccgc tgaaattata attgagcaat ccggcattaa taccgggatc
       61 ccataactca ggaggaatat aaccacgcgc gcgattactc ataaatgcct gagggatcgt
      121 caggttcagt cgctgctgac caacatccag atgcgcagta gcgtcctgga ccattgtggt
      181 taatggcaca caggcatcat ccgccagcag attcataccg gcgacagaag ccgtattcag
      241 ccccatactg gcgagttgcg cgcgtgtcag gcagggaaca atcccttgtt cactgtcgcc
      301 cgtattaaat gtgacatcac gcgttgccat ataaccatta ttcaaataga tatcgacgcg
      361 atacgtccct ggcggtaatt cttgcccatt ttcaaaacgc gataaatcgg ccacagcctg
      421 gggatcatcc gctaaaaagc gcagattaaa atagaggtcg gcagatgaca aaggtgcctg
      481 tgcggcaaaa gcacaggcga caacgagtcg gacaaaaaaa ccagccaaac gatgcttacg
      541 aatatgcaag cattgtgtgt ttcgctggta aagtcttaaa ttcagatatg acattattcc
      601 tccggtttaa aatcacccgg cagtcaattc tttttattca ggcgaaaaat tccccctgcg
      661 ttattccatt acgcccgtca ttttgggggt aagtgcgcca taatcattta ttgttcggta
      721 agtaatattg cttcctgcat cagaaggcaa tttaaccgtg ctttcgccca ttggaggcac
      781 caatgcattt tcaagaaccc gggttccggc attcaactct gttaccgtca ggtaataggg
      841 tgtcgggtta atcagcgtca gagaattcgc gctacgacga aatcttaatt tttctgcggc
      901 ctgatcgggt ggcaacgcta atttagccgg gcgatagtac agtttaatgc ggctgataat
      961 tgcgagctgt agcatattct cagtcaattt tgatttatcc attgacggaa tcgctttaac
     1021 gttcatccag aataaacttt cccggtcctg tggcaattgg ttatttgttg catcaagaat
     1081 acgtaaggta ttctcttttt ttcccttcat cgcaaacaga ggaggcgtca cgataaaacg
     1141 accatccttt acaccatcgg cattttccac ccatgattga attaaatagg tactattttc
     1201 atcattattt atcacggcaa gttgcacttg tttttgccct gccggataaa ttacgcgagt
     1261 cgcacctaag gccactcctg cttcagcgcg tccggcaacc atcattgcca tgaacatcag
     1321 gatacctgcc agcaagcaga atgttatttc ctgcgatttc cttacattga cgtttttatt
     1381 actcactgtc ctgttcctgt tatcacatta tctactgaac aattactgat aggttaaaga
     1441 gaaccaggcc tgggcattgg cgatgccgcc agtaacccga cgcccggtag cacgatattt
     1501 ggcgatgaaa tgtagcgaag tagagcctga gtaaagccgt ttccagtttg ctggaggacg
     1561 attaatcggt acgaggtttc cttcatcatc aaacaacgct acaccaatat tggaggctat
     1621 ccctggcccc tctcccacgg agagcacatc cggattttta ccatccgcga caccgtgaaa
     1681 cgccacaccc acacgttcac tcaccaccgt gctacattcc cgtaaatgaa taacaaaagg
     1741 caccggtgcg ctatcttccc ctgccgcatg aaaccggtta ctgctgattt gccccatatt
     1801 gaccgtcatt tgtttatcac cggcttcaat ccggcaagtt tccgcaataa tgacgccctg
     1861 aaattgcata tttccgccgg gcagcgtggt attccattta tttccggcca gagcaaacat
     1921 tggcagcaac gacgctaaca gaaataatct cttccttttc atcgttattt ttatcgcaca
     1981 agggtgggca tccctgcccg taatgacgtc cttgaacctg ggtaggttat tgatactgaa
     2041 ccttgaaggt tgcatccgca ttagctgcac ccggggttgc ctcgccgatt gcataataac
     2101 gcgcctggaa cggaatggtg ttggtaccgt tattcagggt tgtttgtgca ctaaatgtcg
     2161 caccgtccag cgtcagagca ttgcctgttc tgtctaggat ctgcacgcca acgtttgttg
     2221 cactacctgc agcagaactc tgcagagcca gtacatcagt acgcgtagca tcaattgccg
     2281 tacctaagaa ggcaacagcg gcttttgtgg caacagtggt atcgcaatca ttcagctgaa
     2341 tgttaaaacc aacggcagag ctggttgctc cagcctgctt caggctagcg gtacgaacct
     2401 gtcccaactg aacggtttga tcaacagagc ctgcatcaac tgcgcaagcg gcgttaacaa
     2461 cttccccttt aaagtgaatg gtcccaccat ttaccgtcgt agtatcggcc agagccgctg
     2521 cggaactgag ggacagagcc gacagaacaa cgattgccag agttttaatt ttcatgctgc
     2581 tttcctttaa aaaaactatt tctaaatcga catgggcagt cgttctgtac actttgtttt
     2641 gtcaacgagt ttgactgcca acactgcaca gttttccccc aaaagatgaa acatttgggg
     2701 ccattttgac tcatagagga aagcatcgcg cacaaacttt ttcagtttat ttgttggctt
     2761 aatattctat tattatcttt atttatagat ttttatattg catgaggtgg tttttggaga
     2821 gaagaatgag gaagatgcgt cgagccacag a
//
LOCUS       DAEBXN010000264         2807 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.267, whole genome shotgun sequence.
ACCESSION   DAEBXN010000264 DAEBXN010000000
VERSION     DAEBXN010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.267"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            6..81
                     /locus_tag="KJL99_004420"
     tRNA            6..81
                     /locus_tag="KJL99_004420"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="KJL99_004421"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="KJL99_004421"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(299..1057)
                     /locus_tag="KJL99_004422"
     CDS             complement(299..1057)
                     /locus_tag="KJL99_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBD4438890.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQATPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(1065..2168)
                     /locus_tag="KJL99_004423"
     CDS             complement(1065..2168)
                     /locus_tag="KJL99_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBD4438891.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGVCWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2178..>2807)
                     /locus_tag="KJL99_004424"
     CDS             complement(2178..>2807)
                     /locus_tag="KJL99_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBD4438892.1"
                     /translation="FILAVVMAIVLSVGLFRFNKTYQIKTGQLRRSWPIAAVLIIGLP
                     LLAQWLFGAALHWDVPALQGFNFRGGMVLIPELAALTLALSVYTSAFIAEIIRAGIQA
                     VPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSSLAAVIGYPDM
                     VSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVER"
BASE COUNT          764 a          739 c          674 g          630 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gttgcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt ggggtctccc catgcgagag tagggaactg ccagacatca
      241 aatcaagcga aaggccatcc gaaaggatgg cctttttgct tttcgaacta acattcaatt
      301 aatggattac ctgcgataaa aatgccctcg tacgctctga tttaggatgc gcaaaaaatt
      361 catcaggtgt agcttgttcc actatctccc cacgatccat aaaaattacc cggtcagcga
      421 cggtacgtgc aaaccccatc tcatgtgtta cacacaacat tgtcataccc gactgcgcca
      481 gcccaatcat cgtatccagc acctctttca ccatctccgg atcgagcgcc gacgttggtt
      541 catcaaacaa cataattttc ggcttcatac acagcgaacg cgcaatggca acgcgttgct
      601 gctgaccacc tgaaatctgt ccgggaaact tatgcgcatg ttcggcaatt ctcacccgct
      661 caaggtaatg catcgccaga gcttcagcct ctttcttagg catcttgcgt acccaaatcg
      721 gtgccagggt acagttctgt aaaacggtca gatgagggaa gagattgaaa tgctgaaata
      781 ccattcccac ttcctgcctg acgcgctcaa tattgcggat atcttcatta agttcgatgc
      841 catctacgac aatccgtccc tgttgatgtt cttccagatg attaatacaa cgaatggttg
      901 tcgatttacc ggaaccagaa gggccacaca gaacgatccg ttctcccggt tgcacggtta
      961 aatttatatt tttcaaaaca tggaattgtc cataccattt atggacgttt tccagcgtaa
     1021 tcatcgcgtt agcaggttgc agtaaaattt ggctcatagt gtcctcaatg cggtgtacgc
     1081 ccggtgttaa aacgtttttc cagatgttgg ctataacgtg acatgctgaa acaaaagatc
     1141 cagtagatca gcgcggcgaa aacataccct tccgtcgaca tacccaacca ggcgggatca
     1201 acggttgcct gctgaacgct actgaaaaga tcgaacaagc cgatgatgat cactaggctg
     1261 gtatctttga agagtgcgat gatggtattt accagcccag gaattaccag cttcaacgcc
     1321 tgtggcagaa taaccagccc ctgagttttc cagtaaccca acgccagcga ctctgccgct
     1381 tcatattgcc ctttaggcag cgcctgtaat ccacctcgca cgacttccgc aacatatgct
     1441 gactgaaaca ggatcacgcc aaccagcgcg cggatcagtt tgtcgatact ggtgccttct
     1501 gccataaaca acggcagcat gaccgaagac ataaacaata cggtaatcag cggtacgccg
     1561 cgccagaact cgataaaaat gaccgataag atacgcacaa tcggcatatg ggagcggcga
     1621 cctaatgcca gtaagatccc ccacggtagc gcccccgcaa tcccaacgga tgcaataatt
     1681 aaagttagcg tcagcccgcc ccattgccgg gtttcaaccc gctcaagacc aaaaaaaccg
     1741 ccatacatca gccaccagac aatcagtggg taaatcaccg cccaggccgc aatatagcga
     1801 ccgcgatgcg ggagtatttt ccagaacatt ggtgcgatcg atacaagccc aatcagtaat
     1861 gcgaggttaa ttcgccagcg ttggtcgtgt gggtaaagtc catacataaa ctgaccaaat
     1921 cgttcgtgaa tgaagaccca acagacaccg gcttttgtgc agtcagcacg cgttgagcca
     1981 acccaattag cctgcaaaaa tgcccagttc agcaacggtg ggatcagttc ccacatcaac
     2041 catatgcagc caatagtcag caggctattg ctccagctgg agaacagatt ttttcgcacc
     2101 cacaccatcg cgcggcttga gttatggctc gccgggcgcg agggctgaga cagcaatact
     2161 tttgtcatca tgactcctta gcgttcaacg atcgcgatgc ggcggttata gatattcatc
     2221 agcagcgaga tagtcaggct gataatcaga tagaccgaca tggtcatggc tatcgtctcg
     2281 atggcttgcc ccgtctggtt cagcacggtg ccagcaaaca gcgaaaccat atcgggatag
     2341 ccaataacgg cggcaagaga ggagtttttg acgatgttga gatactggct ggttaacggc
     2401 ggaataatta ctcgcagtgc ctggggaata atgacctggc gtagcgtaac cgggttgggt
     2461 aaccccagtg accgagccgc ttcatgttga ccataaggca ctgcctggat cccagcgcgg
     2521 ataatctcgg cgataaatgc agaggtataa accgaaagtg ccagcgttaa ggctgccagt
     2581 tcaggaatta aaaccattcc gccgcggaag ttaaagcctt gtagggctgg gacatcccag
     2641 tgtagtgctg cgccaaaaag ccattgcgcc agtaaaggca aaccaatgat caacactgcg
     2701 gcgatcggcc aggagcggcg cagttgtccg gtctttatct ggtatgtttt attaaagcgg
     2761 aatagcccaa cagaaaggac tatagccata acaacagcca ggataaa
//
LOCUS       DAEBXN010000265         2772 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.268, whole genome shotgun sequence.
ACCESSION   DAEBXN010000265 DAEBXN010000000
VERSION     DAEBXN010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.268"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(<1..769)
                     /gene="ipaH7.8"
                     /locus_tag="KJL99_004425"
     CDS             complement(<1..769)
                     /gene="ipaH7.8"
                     /locus_tag="KJL99_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HBD4438893.1"
                     /translation="MFSVNNTHSSVSCSPSINSNSTSNEHYLRILTEWEKNSSPGEER
                     GIAFNRLSQCFQNQEAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKEL
                     NADNNRLSVIPELPESLTTLSVRSNQLENLPVLPNHLTSLFVENNRLYNLPALPEKLK
                     FLHVYYNRLTTLPDLPDKLEILCAQRNNLVTFPQFSDRNNIRQKEYYFHFNQITTLPE
                     SFSQLDSSYRINISGNPLSTRVLQSLQRLTSSPDYHGP"
     gene            1098..1277
                     /locus_tag="KJL99_004426"
                     /pseudo
     CDS             1098..1277
                     /locus_tag="KJL99_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1294..1984)
                     /locus_tag="KJL99_004427"
                     /pseudo
     CDS             complement(1294..1984)
                     /locus_tag="KJL99_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1907..2158
                     /locus_tag="KJL99_004428"
     CDS             1907..2158
                     /locus_tag="KJL99_004428"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438894.1"
                     /translation="MPGTTTAMSINFIGITARTMNSNGSHGKPQIPVDYQKLLSIEDI
                     TFCRNRWGNIGENALRRVAVGKKLSFFGSDRGGENAAII"
     gene            complement(2178..>2772)
                     /locus_tag="KJL99_004429"
     CDS             complement(2178..>2772)
                     /locus_tag="KJL99_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HBD4438895.1"
                     /translation="APTLRIRDPHKDEKMAAIHKALDECSAEHPVFYEDEVDIHLHPK
                     IGADWQLRGQQKRVVTPGQNEKYYLAGALHSGTGKVSYVGGNSKSSALFISLLKRLKA
                     TYRRAKTITLIVDNYIIHKSRETQSWLKENPKFRGIYQPVYSPWVNHVERLWQALHDT
                     ITRNHQCRSMWQLLKKVRHFMETVSPFPGGKHGLAKV"
BASE COUNT          751 a          564 c          644 g          813 t
ORIGIN      
        1 gtgggccgtg gtagtccggc gaagaggtta atctttgcag ggattgcaga acgcgagtcg
       61 acaatggatt ccctgaaata ttaatcctgt aacttgaatc taattgtgaa aaactctccg
      121 gaagagtggt tatctgatta aaatgaaaat aatattcctt ttgtctgata ttgtttctat
      181 cagaaaattg aggaaaagta accagattat tgcgctgagc acagagaatt tccagtttat
      241 ccggtaagtc gggtaatgtt gtcagcctgt tataataaac atgtaaaaat ttcaattttt
      301 cgggaagagc cggtaagtta tatagcctgt tattttcaac aaataatgat gttaaatggt
      361 ttggcaaaac aggaaggttt tccagttgat tagaacgaac acttaaagtt gttaatgact
      421 caggaagttc tggtatcaca gaaagcctgt tattatcagc attaagttct ttaagaaatg
      481 caggcagctt tggcaatgat gttaatttat tattttctac aatcaaagca gaaatatgct
      541 ttggtaattc gggaagagac gtcaaattta ggtctgataa atttaatact gcttcttgat
      601 tctgaaagca ctgggagagt ctgttaaaag caatgcctcg ctcttccccg ggagaagagt
      661 tcttttccca ttcagtcagg attctcagat aatgttcatt actggttgag tttgagttaa
      721 tagaggggga gcaagaaact gatgagtgtg tattatttac agagaacata atgctaggtc
      781 aaccttatat ttgtgagaat aaatatttaa aattgtatat ttatacaaat ttcacttacc
      841 atttccgata ttaagtttta atagtttcca ttttacacat aacagaagtt agttgtaatt
      901 tatttatgga ggttttctat ggaattcttt ttttattgtg gctgcagtca ataataacac
      961 tgaattcaac attccagtga gcaaggaaat gtaagccgag taagtatatt tgttttacat
     1021 catattttag tcaagctcat ttattcctat tatagaatga caaattaaaa ttactgtaaa
     1081 aaaacgatac acatttaata cgagggaggc cggcagaaaa acggcttgcg gtcaggcagg
     1141 agaaatgtgt gccgcgaatg aagtccttgt atgactggat aaagctccag aggaaaactt
     1201 tgtcaaacca tgctgaaatg gtaaaggcgt tcaactatat cctgaatcac tggaatgcac
     1261 tgaatgagtt ttgccgggta gtacttccaa tcattagaat atgtgctttt caatgaagga
     1321 accgatgact ttttcgtgga tttccacgga acgcgagaag cagattgttt tacgtgccag
     1381 ccgcttgatg cgggggcgga gggtcaggtt gttacgttca tacgttgcgt gaagattttg
     1441 ccggtcaggt gtttctcctc cggcacttct ctggcgtagc tgccccagtc atcgctggtc
     1501 accatgccga tacagaacgg agtcaacagg gctaacagtt cccgacaggt ctcatcattc
     1561 cgtggcccgc aagtgtaagc caacacaccg ccagttttag tgttgtacgc ataccagggc
     1621 cagtgttgtt gcgccttgct gccaacgaag cgccactgct catcaagttc acagataagg
     1681 gcaacatcag cgtgagcgac tggcgcagat gttatccgcc ggggcgagag ctttttaatg
     1741 tccgtatgac ggtattgatg ccgattttca gtgtcctggc ggtatcacgg ccccctgcgc
     1801 cgttatgtgc catttcaacg atctgctcct taacgccgag ttttctggct tcataagagt
     1861 aagtgagctg gaaaacacgt ttacatgagc gacaacggaa acgctcatgc cgggaacaac
     1921 tacggccatg tcgataaact tcatcggaat tacagcgcgg acaatgaaca gtaacggtag
     1981 tcatggaaaa cctcaaatac cagtagatta ccagaaactt ctatcgattg aagacattac
     2041 cttttgccgc aataggtggg gtaacattgg tgaaaacgca ttacgccggg tggcagttgg
     2101 aaaaaaatta tcttttttcg gctctgatcg tggtggagaa aatgcggcaa taatctaaat
     2161 agctgcgctt aataccgcta cacttttgcc agcccatgtt tgcctccggg gaatgggctg
     2221 acggtttcca taaaatggcg aacttttttc aacagttgcc acattgagcg gcactgatga
     2281 ttacgcgtta ttgtgtcgtg aagtgcctgc catagccgtt caacatgatt cacccatggc
     2341 gagtaaaccg gctgataaat gcccctgaac ttcgggttct ccttcagcca gctctgtgtt
     2401 tcccggcttt tgtggataat gtagttgtcc acgatcagcg tgatggtttt cgcacgacgg
     2461 tatgtcgctt taagccgctt cagcaggctg atgaacagcg ccgaactttt gctgttgccg
     2521 cccacatagc tgactttacc tgtcccgctg tgcagcgctc cggccagata atatttttca
     2581 ttctgtcccg gcgtgaccac ccgtttttgc tgtccgcgca gttgccagtc cgcaccgatt
     2641 ttgggatgaa gatggatatc cacttcatct tcataaaaga ccggatgctc tgcgctgcat
     2701 tcgtccagtg ctttatggat tgctgccatc ttttcatctt tatgcgggtc acggatacgc
     2761 agagttggcg ca
//
LOCUS       DAEBXN010000266         2745 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.270, whole genome shotgun sequence.
ACCESSION   DAEBXN010000266 DAEBXN010000000
VERSION     DAEBXN010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.270"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..198
                     /locus_tag="KJL99_004430"
     CDS             <1..198
                     /locus_tag="KJL99_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBD4438896.1"
                     /translation="KLVEGGIDLKDVNVGNMHFSEGKKQISSKVYVDDQDLADLRFIK
                     QRGVNVFIQDVPGDQKEQIPD"
     gene            237..1040
                     /gene="agaC"
                     /locus_tag="KJL99_004431"
     CDS             237..1040
                     /gene="agaC"
                     /locus_tag="KJL99_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HBD4438897.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIRVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            1030..1821
                     /gene="agaD"
                     /locus_tag="KJL99_004432"
     CDS             1030..1821
                     /gene="agaD"
                     /locus_tag="KJL99_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HBD4438898.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASLGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            1822..2577
                     /locus_tag="KJL99_004433"
     CDS             1822..2577
                     /locus_tag="KJL99_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HBD4438899.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDISQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTTGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHSNFICLINT"
BASE COUNT          683 a          635 c          679 g          748 t
ORIGIN      
        1 aaattggtag aaggtggcat tgacctgaaa gatgtcaacg tcggcaatat gcatttctcg
       61 gaagggaaaa agcaaatcag cagtaaagtt tatgtcgatg accaggatct cgcggactta
      121 cgttttatta aacaacgtgg cgtgaatgtt ttcattcagg acgtccctgg cgatcaaaaa
      181 gaacaaatcc ctgactaaat ctaaaatcgc cttaatattg gtttgaggta ataaaaatgc
      241 atgaaataac cctacttcag ggattatccc tagcggcgtt agttttcgtt ctggggattg
      301 atttttggct ggaagcctta tttttattcc gcccgataat cgtttgtacc ctaactggcg
      361 ctattctcgg tgatattcag actggcttaa ttaccggtgg tctgacagag ttggctttcg
      421 ccggattaac ccctgcaggt ggtgttcagc cgcccaaccc gattatggcg ggtctgatga
      481 ccaccgtcat tgcatggtct acgggcgttg atgccaaaac agcaattggt cttggcctgc
      541 cgtttagttt gttaatgcag tacgtcattc tgttcttcta ttccgctttc tcattattta
      601 tgaccaaagc cgataaatgc gcgaaagagg cggatacggc agcgttttcc cggcttaact
      661 ggacaacgat gctcatcgtc gcttcagcgt atgcggtgat tgctttcctc tgtacttacc
      721 tggcacaagg ggcgatgcag gcgctggtga aagcgatgcc cgcctggttg acccacggct
      781 ttgaagtggc tggcggtatt ctgcctgccg ttggttttgg cttgctgctg cgcgtaatgt
      841 tcaaagcgca atatatccct tacctgatcg ccggtttcct gtttgtttgc tacatccggg
      901 tcagcaacct gttgccggtt gccgtactgg gcgcaggctt tgcggtgtat gagtttttca
      961 atgcgaaatc ccggcagcaa gcgcaaccgc agcccgttgc cagtaaaaat gaagaagagg
     1021 actacagcaa tgggatctga aatcagtaaa aaagatatca cccgtctggg ctttcgttcg
     1081 tcgctgctgc aagcgagctt taactacgaa aggatgcagg cgggcggttt tacctgggcg
     1141 atgttgccga tcctgaaaaa gatttataag gacgacaaac cgggcttaag cgcggcgatg
     1201 aaagataacc tcgaatttat taatacccac ccgaatctgg tcggattcct gatggggtta
     1261 ttaatttcga tggaagaaaa aggagaaaac cgcgacacca ttaaaggcct caaagtggca
     1321 ctgtttggcc caatcgccgg gattggcgat gcgattttct ggtttacttt gttgccgatt
     1381 atggcgggaa tttgctcatc atttgccagc ctgggaaacc tgctggggcc gattctattt
     1441 ttcgccgttt acctgcttat ctttttcctg cgcgtcggct ggacccacgt cggttattca
     1501 gtcggcgtga aggcgatcga taaagtgcga gagaactcgc agatgattgc tcgctcggca
     1561 accatcctcg ggatcacggt aatcggcggg ctgatcgctt cgtatgtgca tattaacgtg
     1621 gtgacatcgt ttgccatcga cagtacccac agcgtcgcgc tgcaacagga tttcttcgat
     1681 aaagtcttcc cgaacatttt accgatggcc tacaccctgc tgatgtatta cttcctgcgg
     1741 gtgaaaaaag cgcatccggt gctgttaatc ggcgtgactt ttgtgctctc tattgtttgt
     1801 tccgcattcg gcattttgta aatggaacga ggcactgctt ctggcggtgc ctctttactt
     1861 aaggaatttc atcctgtgca aacccttcag caagttgaaa actatacggc gttaagtgaa
     1921 cgagccagcg aatatttatt ggctgtgatc cgtagcaaac cggatgccgt gatttgcctg
     1981 gcgaccggag ccacgccatt actgacgtat cattatctgg tagaaaaaat ccaccagcag
     2041 caggttgata tcagccagct caccttcgtg aagctcgacg aatgggtgga tctgccatta
     2101 acgatgcctg gcacctgcga aactttccta caacagcata tcgtgcagcc gctggggcta
     2161 cgtgaagacc agctcatcag ctttcgctcc gaagagataa atgagacaga gtgcgaacgg
     2221 gtaacgaacc tgattgcgcg caaaggcggt ctggatttat gcgttctcgg attggggaaa
     2281 aacggtcatc ttgggctgaa cgaaccggga gaaagcctac aaccggcctg ccatatcagt
     2341 caacttgatg ccagaacaca gcaacatgag atgttaaaaa ccacgggtcg ccccgtgact
     2401 cgtgggatca ccttaggcct gaaggatatt ctcaatgccc gcgaggtttt gttattggtg
     2461 actggcgaag gaaagcagga tgcgacagaa cgttttctca cggctaaagt ctccaccgct
     2521 atcccggctt catttttatg gctgcacagt aactttattt gtttaattaa tacctaacca
     2581 gatgaaaaat ttgctcaaaa tttttaaatt aacttattca acagtcacgc attatattaa
     2641 ataacatttg actgggttga acataacgcc aatagcaaaa ggagttatgt tcaacaattt
     2701 aattttagat atacatataa cttgttctat taataaatca cctcg
//
LOCUS       DAEBXN010000267         2734 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.271, whole genome shotgun sequence.
ACCESSION   DAEBXN010000267 DAEBXN010000000
VERSION     DAEBXN010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.271"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            88..153
                     /locus_tag="KJL99_004434"
                     /pseudo
     CDS             88..153
                     /locus_tag="KJL99_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
     gene            381..629
                     /locus_tag="KJL99_004435"
     CDS             381..629
                     /locus_tag="KJL99_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HBD4438900.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(745..1014)
                     /locus_tag="KJL99_004436"
     CDS             complement(745..1014)
                     /locus_tag="KJL99_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HBD4438901.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQGFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDTM"
     gene            1071..1739
                     /locus_tag="KJL99_004437"
     CDS             1071..1739
                     /locus_tag="KJL99_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBD4438902.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            2055..>2734
                     /locus_tag="KJL99_004438"
     CDS             2055..>2734
                     /locus_tag="KJL99_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HBD4438903.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTL"
BASE COUNT          836 a          589 c          537 g          772 t
ORIGIN      
        1 atagatgcac aaaaaacggg caaaactacc tggttcgcaa aactgcgtct aaagttaaac
       61 tgggacctcg cgagcaaggg tgagacgatg gcgctttaca caattggtga agtggcgttg
      121 ctttgtgata ttaatcctgt cacgttacgc gcgtgacgca acatttttaa aaatcgacga
      181 agatcgtcca ggagcgccgt ttttcaaggg ttggatagat gattcgttta ccatgtcgac
      241 gaacttcgac gaatttcgcc gataaaaagt gtcccataca tgccccattg atgccccata
      301 aacaatatca ggaattgaca ctggttatcc atacagataa aaataacacc tgtacataac
      361 tacagttaca ggagggtgtt atgcgtgttg agatctgtat cgctaaagaa aaaatcacta
      421 aaatgccaaa cggtgctgtg gatgcgttaa aggaagaatt aacccgacgc atcagtaaac
      481 gttatgacga tgtagaggtg atcgtaaaag ccaccagcaa cgatggcctt tctgttacac
      541 gcaccgcaga taaggattct gcaaaaacat ttgttcagga gactctgaaa gatacctggg
      601 agtctgctga cgagtggttt gttcactaat aaacacgtaa aatcggtaac ggctggaaat
      661 cattcaatac tcgcactatc gaaagtttac cagccaaccg cagcacatcc tgcatacgtc
      721 gtgtctgcgg tttttctttt tcgcttacat tgtgtctggt tcttccggcc actcaatatc
      781 aggtgcagtt gatgtatcaa cacggttcag caacacccga tacttcttcc aggcttccag
      841 caacgagttt tcttcctccg ttgcgatctc cagatctaca gcatcctgaa gtggcgcaat
      901 atgctcactg aatccctgga tgtagaactg tgtggtgacg gtcttccagc cattcggctc
      961 ctgctgtatc gaagcatacc aggctatttc aatatcgcta tgctgcggca gcatttaacc
     1021 ccttgtaatt catcgccata attgatttaa ttcacaaata aaactataat atggtgaaat
     1081 taatgaaaaa aaacacagat gatggggcta aaatttacac accacttacc ctaaagcttt
     1141 atgactggtg ggttttggga gtatcaaatc ggcttgcatg gggatgtcct acaaaggaac
     1201 accttcttcc acactttctg gaacatttag gtaacaacca tctggatatt ggtgttggaa
     1261 ctgggtttta ccttactcac gtacctgaga gtagtctgat atctttaatg gatttgaacg
     1321 aagctagcct gaacgcggca tctacaaggg ctggggaatc aaaaattaaa cataaaatta
     1381 gccatgatgt ttttgaacct tatcctgcgg cgttacatgg tcaatttgat tccatttcca
     1441 tgttttacct tcttcactgc ctgcctggaa atatatctac aaaaagctgt gtaatacgca
     1501 atgcggcgca ggccttaact gatgatggaa ctctatacgg agccacaatt cttggcgatg
     1561 gagttgtgca caatagcttc ggtcaaaaac tgatgcgcat ttacaatcag aaaggcatct
     1621 tttcaaacac aaaagattcc gaagaaggct taacacatat actctcagag catttcgaga
     1681 atgttaaaac caaggttcaa ggtactgtag taatgttttc cgcttcaggg aaaaaatagc
     1741 atccaaccgc agcacgttct tgcttaagac gtgctacggt ttcctttacg atttggggtt
     1801 gcgactttac ctctattgat aatgcattcc ggcagaacgt tcaaatatga acgtacgata
     1861 tttaactaac cgaaaaacaa aatatagcac aggcgagaca tttaccatct aagaaaatag
     1921 tatcgttttt ttacagttaa atcaacatca cttccttaaa atgaaaacaa taaatttcaa
     1981 tcctgaaaca ttttatttca gcttattatt ccccagtatc acattttatc aggatatcct
     2041 tctgcgaggt tattatgctt cctgtaaata atccccccct atccactgga aacgtctctt
     2101 tttacagaac tacatcaatc gacaatgttc acaataatta tctctccgaa tgggttgaat
     2161 ggactaaaaa cagcatttcc ggagaaaaca gggaaactgc ttttacccgg ctccaattat
     2221 gtctggagaa cagtgaaaca tcgttggact tatcttgttt aggtctcaga tctctaccac
     2281 gattgcctga caatcttgat gaaattaatg taagcaataa ccaactatca atgctccccg
     2341 agctaccaag ggcattgaaa gagctgaatg caagcagtaa tcaattatct gcacttcctg
     2401 aattaccagt gtcgctggaa tatataaatg tgagtgataa ccatttgttc gcacttcctg
     2461 aattacctgc gtcactagaa tatattaatg taagtgacaa tcacctgtct gtacttccga
     2521 ggttaccaat gtcattggaa ttacttgatg cagccagaaa tgctttggaa gtaataccag
     2581 attttccaga aagagatgat catattataa gaatattctg gcttaatcag aaccggatca
     2641 cggcaattcc ggaaagcata cttggcctca gttctgatag cgttgtcaat cttagagaaa
     2701 atcaactatc tcccagaata atgcaaactt tgtt
//
LOCUS       DAEBXN010000268         2715 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.272, whole genome shotgun sequence.
ACCESSION   DAEBXN010000268 DAEBXN010000000
VERSION     DAEBXN010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.272"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            53..2575
                     /locus_tag="KJL99_004439"
     CDS             53..2575
                     /locus_tag="KJL99_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBD4438904.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEINHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIHQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGLTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKLFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAARLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSFSTPESESGLIEQGVTCH"
     gene            2586..>2715
                     /locus_tag="KJL99_004440"
     CDS             2586..>2715
                     /locus_tag="KJL99_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBD4438905.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHSTLDFS"
BASE COUNT          727 a          586 c          679 g          723 t
ORIGIN      
        1 cagtgacccg ataaaaatga cgctgtaagc aagggataag gataggcgta ttatgatgac
       61 gaccagaata gtggttggcc tcacggcagg gacgtgtctg attttctcgc aaaacctgat
      121 ggccgaggtc agtgtattca atccggcgct tctggaaatc aaccatcaat ccggagtcga
      181 tattcgccag tttaatcggg caaacctgat gcccccaggt gtttatagcg ttgatatttt
      241 tatcaacggt aaaatgtttg aacgtcagga tgtgacattt gttcaggata atccagatgc
      301 tgatctgcac gcttgcttta ttgccattaa aaaaacactg tcctcctttg gcataaaagt
      361 tgatgcgctc aaatcgttca atgatgtgga tgagacggtt tgcctcgatc ctgctccacg
      421 tattgaaggc tcatcctggc agtttgacag tgataaattg cagctgaata tatccattca
      481 ccaaatctac atggacgcga tggcttatga ttacatcagc cccacgcgtt gggatgaggg
      541 gattaatgcg ctcaccatca actacgattt ttctggttca catacactac gttcagatta
      601 tggttcacaa gagacagata ccagttatct caatctgcgc aatggactga atattggacc
      661 gtggcggcta cgtaattaca gtactttaaa caccagcgat ggccgtgcgg aatacaactc
      721 cattagtacc tggatacagc gcgatattgc cgcgttaaga agccagatta tgattggtga
      781 tacgtggacg gcgagcgata ttttcgacag tacgcaaatt cgcggcgcgc gtttgtatac
      841 tgataacgat atgctacccg ccagccagaa tggctttgct cctgtggttc gtgggattgc
      901 aaagtccaac gccaccgtca tcattcggca gaatggctac gtgatttatc agtcagccgt
      961 tccacaaggt gcttttgaga tcaccgatct caacaccgca agtacaggtg gcgatttgga
     1021 cgtaaccatc aaagaagaag acggtagcga acaacgattc acccaacctt atgcttcatt
     1081 ggcgattctt aaacgtgaag gtctgacaga tgttgatgtc agcgtgggtg aattgcgcga
     1141 tgaagacgga tttacaccgg acgtccttca ggcgcaaata cttcatggtt tttcccacgg
     1201 gatcacttta tatggaggta tgcaggctgc tgaaaattat ggttctgcag ctctgggtgt
     1261 cggtaaagat cttggcgctt tgggcgcaat ttctttcgat gtgacacatg ctcgtgcgaa
     1321 ttttagccat gatgatacag aaacgggtca gtcatatcgc tttctctatt caaaactatt
     1381 tgacgacaca gacactagct tgcgcctggt tggctatcgt tactccaccg agggctacta
     1441 taccctcaat gagtgggcat cgcggcgcaa cagccctgaa gacttttggg aaacaggtaa
     1501 ccgacgtagt cgcgtggagg gaacgctaac gcagtcgttg gggagagatt atggcaattt
     1561 atacctgaca ttaagccggc aacaatactg gcataccgat gatgtcgaac gattaatgca
     1621 atttggctac agcagtagct ggaagcgtct ctcgtggaac gtctcctgga gttattccaa
     1681 tactgccaga caggggacgg ggaacaacca tgccagtgat aacaccagtg agcagatcta
     1741 catgctctct ttatctgttc ctttatcggg ctggtggggt aatagttacg ccacctattc
     1801 tgtttcgcaa aacgataatt ccggtagctc acatcaactc ggactcagcg gtacggcgct
     1861 ggaaagaaat aacctttcat ggaatttaat gcagtcctat aacagtcatg atgatgaggt
     1921 tggcggtaat atgtccctga cctatgatgg ctcttatggc acggtgaacg gcagctataa
     1981 ctacagccaa aattcccaga ggctgaatta tggtatcaga gggggaattc tggcacacag
     2041 cgaaggggta acgttaagtc aggagttagg tgaaactatt gctcttgtta aagcacctgg
     2101 ggccgccagg ttagaaatag ataatatgcg cggtgctgcg acggactggc gcggctatac
     2161 ggtcaagaca cagctaaacc cttatgatga aaatcgggta gcaatcagcg ataactattt
     2221 ctcgaagtcg aatatagaac ttgataatac cgtcgttacg atggttccca cgcgtggtgc
     2281 agtggttaaa gcggagtttg tgactcatgt gggttatcgc gttctcttca gagtgttaaa
     2341 tgcaaatggt aaaccggtac cttttggagc cattgctgcg atacaagatg caagtttggc
     2401 agattcagga attgtcggtg accgtggcga actttatctt tctggtctac cagaaaaagg
     2461 acaggttacg ttatcctggg gagaaaacgc ctcaacaaaa tgcatcttca attattcatt
     2521 ttcgacacca gaaagtgaga gcggattaat tgaacagggt gtgacatgtc attaaaagga
     2581 tttttatgaa caagtatatc aaacagtggt gctttgctgt gtttatgctc tcgttaagta
     2641 gtgtagcact tgcagctcct aaaggtatct gtacccctga taatggcgta tttcacagta
     2701 cgcttgattt ctcgg
//
LOCUS       DAEBXN010000269         2631 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.273, whole genome shotgun sequence.
ACCESSION   DAEBXN010000269 DAEBXN010000000
VERSION     DAEBXN010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.273"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            35..1087
                     /locus_tag="KJL99_004441"
     CDS             35..1087
                     /locus_tag="KJL99_004441"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HBD4438906.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            1163..2596
                     /locus_tag="KJL99_004442"
     CDS             1163..2596
                     /locus_tag="KJL99_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HBD4438907.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKLIAYLLIGKIGNTMLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
BASE COUNT          621 a          587 c          681 g          742 t
ORIGIN      
        1 caataagtta tgtttttaaa ttgagggcat tattatgaaa aaaataccct gcgtgatgat
       61 gcgaggtgga acctcgaggg gcgcgttcct gttagcggaa catttacccg aagatcaaac
      121 gcagcgcgat aaaatattga tggcaattat gggttccggt aacgatctgg aaattgacgg
      181 tattggcggc ggtaatccac tgaccagtaa agtcgccatt attagccgtt ccagcgatcc
      241 gcgtgctgat gtcgattatc tgtttgctca agttatcgtc catgagcaac gtgtcgatac
      301 cacgcctaac tgcggcaata tgctgtctgg cgtaggggca ttcgccattg aaaatggttt
      361 gattgcagcg acttcgccag ttacccgtgt acgtatccgc aacgtcaata cgggtacgtt
      421 catcgaagct gatgtgcaaa cgccaaatgg tgttgtcgag tacgagggta gcgccagaat
      481 tgacggcgta ccgggtactg ccgcaccggt tgcgctcact ttcctgaatg ccgctggaac
      541 gaaaactgga aaagttttcc cgactgataa tcagattgat tattttgacg atgtcccggt
      601 gacctgtatc gatatggcga tgccagtcgt cattattccg gctgaatatc tgggaaaaac
      661 aggttatgaa ttaccggcgg aactggatgc cgacaaagca ttattagccc gcattgaatc
      721 tatccggcta caagcgggta aagcaatggg cttaggtgat gtcagtaata tggttatccc
      781 taaacctgtg cttatttctc cagcgcagaa aggcggggca attaatgtgc gttattttat
      841 gccgcattct tgccatcgcg cgctggcgat aaccggtgct attgctattt ccagtagttg
      901 tgcattggaa ggcaccgtca cccgacaaat cgtcccttct gtaggatatg gcaatatcaa
      961 tattgaacac cccagtggtg cgctcgacgt tcatttaagt aatgaaggtc aggatgccac
     1021 gacgttacgc gcatctgtta ttcggacaac cagaaaaata ttttccggtg aagtttatct
     1081 tccctgaaaa aattcgttgt caggataagg acaatcaata aaggacttct gtatgagtca
     1141 tacagaaaga acaggatttt aaatgaataa gaaatcgtta tggaagctaa ttctgatatt
     1201 agcgatccca tgtattattg gttttatgcc agctccggca ggattaagcg aactggcgtg
     1261 ggtgcttttt ggtatttacc tggcggccat tgtgggtctg gttatcaagc ctttcccgga
     1321 acctgtcgta ctgttaattg ccgttgctgc ctcaatggtg gtggtcggta acttatccga
     1381 cggtgcgttt aagaccaccg ccgtattaag cggttactct tcaggtacta cctggctggt
     1441 gttctcggcg tttaccttaa gcgccgcatt tgtgaccacc ggtttaggta aacttattgc
     1501 ctatctgctg attggtaaaa tcggtaacac catgctgggt ctgggttacg ttacggtatt
     1561 cctcgatctg gtactggctc cggcaacacc gtctaacacc gcgcgtgcgg gcggcattgt
     1621 gttaccgatc atcaacagcg tggcggtggc tttggggtcc gaaccggaaa aaagtccgcg
     1681 tcgtgtcgga cattacctga tgatgtccat ttacatggtc accaaaacca ccagctatat
     1741 gttctttacc gcaatggcgg ggaacattct ggcgctgaaa atgatcaacg acattctgca
     1801 cctgcaaatt agctggggtg gatgggcgct ggccgccgga ttgccgggca tcattatgct
     1861 gctggtcacc ccgctggtaa tttacaccat gtatccacca gaaattaaaa aggtggataa
     1921 caaaaccatc gctaaagcgg gccttgccga actgggaccg atgaaaatcc gcgaaaaaat
     1981 gctgctcggt gtctttgtgc tggcgctact gggctggatt ttcagtaagt ctctgggggt
     2041 tgatgaatcc actgtggcaa tcgttgttat ggcgaccatg ctgctgctgg gtatcgttac
     2101 ctgggaagac gtggttaaaa ataaaggcgg ctggaatacc ttaatctggt acggcggtat
     2161 tatcggctta agctccttat tatcgaaagt taaatttttc gaatggttag ctgaagtctt
     2221 taaaaataac ctggcatttg atggtcacgg taacgttgct ttcttcgtta ttattttcct
     2281 cagcatcatc gtgcgttatt tcttcgcttc cggtagtgcc tatatcgttg ctatgttacc
     2341 tgtatttgcc atgctggcga acgtctccgg cgcaccgtta atgttaaccg cgctggcact
     2401 gttgttctcc aactcctatg gcggcatggt tactcactat ggcggcgcgg caggtccggt
     2461 catctttggc gtgggttaca acgatattaa atcctggtgg ttggtcggtg cggtactgac
     2521 gatattaacc ttcttggtgc atatcaccct cggcgtgtgg tggtggaata tgctgatcgg
     2581 ctggaacatg ctgtaaatat actcgtcata cttcaagttg catgtgctgc g
//
LOCUS       DAEBXN010000270         2547 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.274, whole genome shotgun sequence.
ACCESSION   DAEBXN010000270 DAEBXN010000000
VERSION     DAEBXN010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.274"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(353..1132)
                     /gene="ipaJ"
                     /locus_tag="KJL99_004443"
     CDS             complement(353..1132)
                     /gene="ipaJ"
                     /locus_tag="KJL99_004443"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HBD4438908.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAL"
     gene            complement(1732..1869)
                     /locus_tag="KJL99_004444"
     CDS             complement(1732..1869)
                     /locus_tag="KJL99_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438909.1"
                     /translation="MNVQAIFILMVVQLFGKEIAVTIEELQAVLVPHRLLSQLVKFRS
                     A"
BASE COUNT          768 a          630 c          403 g          746 t
ORIGIN      
        1 acttataata tccatacccc tcatagggct tacagtttaa attgcgggta taaaatacat
       61 ctattttcaa ataaaataaa gcatactcca attacaacga gtgactaaaa taaaaatatc
      121 atccaagtca atcactgttt gctctgcaaa atatgtcagc cttctcaaac ttaccagcac
      181 ttaaatattt aagcacacaa tcattccttg agggagttca cttatttcaa gagaaacatt
      241 tcgtcttccc acacaactaa tccgcccaat atagctaaat ttgtactcag taatatcatt
      301 tgttatgccc ccccaaaata ttcaagctcc ttcacagata aaaaacgaca atttacaaag
      361 cctcattagt tataactatg gaaataccta ttttagtata tcctataaag ttagaattac
      421 ttcttcttgc acctaattcc atagttgaaa aacaagaata attttctcct acagctggat
      481 ccatatagct tccatcaggt ctacacaaca cccagtggag gccgacagga actccaataa
      541 aggatacagc aacagcttcc aaaactcttt ggttagaaga taacacatct cgttgatgta
      601 ctatattaca tcccatgcca attaataagt cccttgcatc gggataaata aaactaatta
      661 cttgcgaaaa aatgttactt tcttcaacga cataagcatt caatccaaga agcctcgtag
      721 ccatgactat cttatcaggc atagaataac cggcatccgc gatattatct ttgtgtattc
      781 tcggattata attaccacta gttattaagt ataaatcccg ctcacaacga ttatccaaat
      841 caagagaact cttccttgtc atttcggaca tagacccttt gtattgtggt attttatcaa
      901 cccctaattc ctttgcagca cagagtaaac tggcagctcc acaagaattg tcatacatct
      961 gcttcctcgc caaaaaacaa ccttgttcgt ttactctatt ctcatttacc ctcacatcag
     1021 tctgatgaga tatcatatat tctcttggta tagccccctg caataaaacc ccatataaca
     1081 taacacctgt atgaatacat ccccgtttgc aaggcttccg ttgttccgac atcgacgagt
     1141 aattgcagca cagtattgca tccagcaatg cgaactacta tgagaattag ctgaccgggt
     1201 ccttattccc cgaacgaact ttaaatccaa aacctcatac tcttttccgt cgtcatcacg
     1261 tccgtcacca taacaacctt tcagcatcca cccttttatt aagagttaat gtccatcagc
     1321 atcagcaatt aataccgcct gatactgaca tatttttgtc gtatttacgt ccataatcgg
     1381 ttattgtgat acaggtcaca ccagcttatt tgctattgtt acaataacca ccttggttga
     1441 gagctgtttt ttcaactttg ttatcaactc ccccaactcc ttggtcagta aggctgccga
     1501 ccgagcacag tgaatagata gtatccgcag gtaccaccat gcttacttaa tactcagaag
     1561 gcgaagtgct ctccaattaa ctctgccgca ctatgcttat aagctgcttt tcaatgccgt
     1621 ctatcaacat gtccagttca ttcaatctgt tgtactggta atccaacgca gtaatgatat
     1681 atgcaggtat tctttcatcc actccagtaa cgtttcatgc aaagcattaa tttacgcgct
     1741 acggaacttc accagttgac tgagcaaacg atgtgggacc agtactgcct gcagttcttc
     1801 tattgttaca gcgatttctt taccaaacag ctgaaccacc atcaagataa agatagcctg
     1861 aacattcatc gcaccatttt gttaccaatg gcaaaagatt taacgaaccg tgcctgcatc
     1921 aggcggactt tttatccctg ctttgttagc tccctaacct tgtacagaga gcctccataa
     1981 gctcccatat caagcagaca tggttcgcag ttactgacat acaccagaaa actctgacag
     2041 cagactcact tatcctccac ttcactcata tccttattgg tgagatgaac ctgaatgaca
     2101 tgttttgcga cataaacgcc aaccagtgta tatttcacct gtagagattc tacaatctgt
     2161 agcgttatgc atctaatgtt aggctctatg atgtcagaaa tctgcgagac tctacacctt
     2221 ctgcatttac ctttatttat caccatgctc ctcagttaac ggagaacgtg ttcattgcat
     2281 tttgtcactt acatttcgcc tttggtatcg gggggatata ggttccaacg ttccgcatga
     2341 gaacctgaac cggcttcacg ccaccttgat accagatgcc gcacaggtag tcaacaagtg
     2401 tcacgaacgg tgcaatagtg atccacaccc aacgcctgaa atcagatcca gggggtaatc
     2461 tgctctcctg attcaggaga gcttatggtc acttttgaga cagttatgga aattaaaatc
     2521 ctgcacaagc agggaatgag tagccgg
//
LOCUS       DAEBXN010000271         2544 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.275, whole genome shotgun sequence.
ACCESSION   DAEBXN010000271 DAEBXN010000000
VERSION     DAEBXN010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.275"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            126..257
                     /locus_tag="KJL99_004445"
     CDS             126..257
                     /locus_tag="KJL99_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
                     /protein_id="HBD4438910.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNAFICAGSELIVARMGSWPEVVMA"
     gene            517..1512
                     /gene="pgl"
                     /locus_tag="KJL99_004446"
     CDS             517..1512
                     /gene="pgl"
                     /locus_tag="KJL99_004446"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HBD4438911.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESVLPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDYSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(1553..2506)
                     /locus_tag="KJL99_004447"
                     /pseudo
     CDS             complement(1553..2506)
                     /locus_tag="KJL99_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
BASE COUNT          635 a          578 c          628 g          703 t
ORIGIN      
        1 ctgttttttt ctttaaaaaa acagtacatt ctattttata atgactgtgt attcccatgg
       61 aaggagatca cccaattctc ccccccgact tttgacgcag tgtcttgtta acagggggat
      121 tgaccatgct tattggctat gtacgcgtat caacaaatga ccagaatacc gatctacaac
      181 gtaatgcgtt tatttgtgcc ggaagtgagt tgattgttgc gcgaatggga agttggccag
      241 aagttgttat ggcatagtgt ttacggtcgt tgatacaggt aaacaaaagc ggggagagtc
      301 atactccctg aatagtcgcg aaaatatctg tggcattgtc aaattcgtgt cggtgactaa
      361 tattttagta atatgccatt cgaaatggag tgctaactta ttgatttaat tgatgtgcga
      421 tattgtattt tatcgtgttt tgttgtctgt tttttatcca gtaagtgttg ttatatgtat
      481 gaaagataag ggatttatca tttttgaggt gtgttcatga agcaaacagt ttatatcgcc
      541 agccctgaga gccagcaaat tcacgtctgg aacctgaatc atgaaggcgc actgacgctg
      601 acacaggttg tcgatgtgcc ggggcaggtg cagccgatgg tggtcagccc ggacaaacgt
      661 tatctctatg ttggtgttcg ccctgagttt cgcgtcctgg cgtatcgtat tgccccggac
      721 gatggcgcac tgacctttgc cgcagagtct gtgctgccgg gtagtccgac gcatatttcc
      781 accgatcacc aggggcagtt tgtctttgta ggttcttaca atgcgggtaa cgtgagcgta
      841 acgcgtctgg aagatggcct gccagtgggc gtcgtcgatg tagtcgaggg gctggacggt
      901 tgccattccg ccaatatctc accggacaac cgtacgctgt gggttccggc attaaagcag
      961 gatcgcattt gcctgtttac ggtcagcgat gatggtcatc tggtggcgca ggaccctgcg
     1021 gaagtgacta ccgttgaagg ggccggcccg cgtcatatgg tattccatcc aaacgaacaa
     1081 tatgcgtatt gcgtcaatga gttaaacagc tcagtggatg tctgggaact gaaagatccg
     1141 cacggcaata tcgaatgtgt ccagacgctg gatatgatgc cggaaaactt ctccgacacc
     1201 cgttgggcgg ctgatattca tatcaccccg gatggtcgcc atttatacgc ctgcgaccgt
     1261 accgccagtc tgattaccgt tttcagcgtt tcggaagatg gcagcgtgtt gagtaaagaa
     1321 ggcttccagc caacggaaac ccagccgcgc ggcttcaatg ttgattacag cggcaagtat
     1381 ctgattgccg ctgggcaaaa atctcaccac atctcggtat acgaaattgt tggcgagcag
     1441 gggctactgc atgaaaaagg ccgctatgcg gtcgggcagg gaccaatgtg ggtggtggtt
     1501 aacgcacact aaccgctgat ttaaccggcg cagtctctcc tgcgccggtg tattaactta
     1561 tctcctgtaa cgcatgtctc tggcgttcga cgatattggt ccacaaattg tctttatcgt
     1621 cagtccacaa attaatcagc aaggaaaaaa agcgttcagc tgccggagaa agcacggcat
     1681 ctttgcggcg aataatcccc aatgtccgac gtataacggg ttccaccagt gggataccaa
     1741 taatcgaaga atagggcgca tggggcattg ccagaccagg aagcgccgaa atccccagtc
     1801 ctgcctccac tagtcctaat gacgtcgaaa gatgacgcac ttcgtaaaac cagtccagct
     1861 tccagggctt gtcggctagc tgttgttcta tcaacaatcg gttgccgctg gaggaacgta
     1921 cgccaatcat tttgtaaccc actaactcct gccattctac aagctacttt ttggccaatg
     1981 gatggtcacg ccgacaggcc agcacgaatg gctcgttgac cagtggggta aaatcaatgg
     2041 atgaatttgt gacgttgttc atgtttatgc caaagtctga ttcgttacac agcaccgatt
     2101 ccatgcaatt attcgtgcct tgttccagaa tccgcacttt aatattggga tacagctcat
     2161 taaatttacc gattgccagc ggtaaaaaat aaaatactgc ggtcggaata cacgctaatg
     2221 tcaccatacc acgatgataa gcattcatat cacgaatatt aaaaagcgtt tcatcgaatt
     2281 tctttattaa ttcccgcgcc tccgggagca acctttttcc tgcttttgtt aacgtaacct
     2341 tgcgagttgt acgctcaaat agttgaatat gtaaatcttc ctccatcttt tttattcttc
     2401 gcgttaatgc aggttgcgta atattgagta atttagcggc attgttaaat gaactggact
     2461 ctgccagtat gacaaatgcc ttcatacttg ataattcatg cttcattatt actccggaaa
     2521 atggaagcga cgattttggg gggc
//
LOCUS       DAEBXN010000272         2492 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.276, whole genome shotgun sequence.
ACCESSION   DAEBXN010000272 DAEBXN010000000
VERSION     DAEBXN010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.276"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..>2492
                     /locus_tag="KJL99_004448"
     CDS             <1..>2492
                     /locus_tag="KJL99_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBD4438912.1"
                     /translation="QEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMGLRFGRDV
                     NAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVITEEWRAH
                     QDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQPNNMTFIV
                     VGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRVNGIALYY
                     RLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVKIAPDYQS
                     LFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAVDQQAERD
                     LRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQDAFWEQM
                     VNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEISSKETLAE
                     NLTSLTLSNGAKVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANKAVSGSGV
                     GELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRITHSTINDN
                     IWASLQNAQIQALKTLDQRPAEKFAQLMYETRYADDRTKLLQENQIAQFTAADALAAD
                     RQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRATDNASVTV
                     KEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASGAYSVSSR
                     LSVDPQAKDISHLLAFTCQPERHDEQLTLANEVMVKRLTKGISEQELNEYQQNVQRSL
                     DIQQRSVQQLANTIINSLIQYDDPAAWTEQEQ"
BASE COUNT          730 a          578 c          602 g          582 t
ORIGIN      
        1 caggaagaag acaatgagcg cggcgtggct cattttgtag aacatatgat gtttaacggc
       61 acaaaaacat ggccgggtaa taaagtcatc gaaacatttg agtcaatggg cctgcgtttt
      121 ggtcgcgatg ttaatgccta taccagctat gacgaaacgg tgtatcaggt gagtttgccg
      181 actacgcaga aacaaaatct gcaacaagtg atggcaatct tcagtgaatg gagtaatgcc
      241 gcaacctttg aaaaactcga agtagacgct gaacgtggcg taattactga ggaatggcgt
      301 gcccatcagg atgcgaaatg gcgcacctct caggcgcgcc gccctttcct gctggctaat
      361 acccgtaatt tagaccgtga acctatcggc ctgatggata ctgtcgccac ggtcacgccg
      421 gcacaattgc gccaatttta tcaacgctgg tatcaaccaa ataatatgac ctttatcgtg
      481 gtcggcgata tcgacagtaa agaagcgctg gcgctgataa aggataattt aagtaagctt
      541 ccggctaaca aagcagctga aaatcgcgtc tggccgacaa aagccgaaaa ccacctgcgc
      601 tttaatatca ttaatgataa agaaaaccgg gtgaacggca tcgcactcta ttatcgcctg
      661 ccaatggtac aagtgaacga tgagcaaagc tttgtcgaac aagctgaatg gagcatgtta
      721 gttcagctgt tcaatcaacg tctgcaggaa cgcatacagt cgggcgagtt gaagactatt
      781 tctggcggca ctgcgcgcag cgttaaaatt gcacccgatt atcagtcgct gtttttccgt
      841 gtaaatgcac gagacgataa tatgcaggat gctgcgaatg cattaatggc agagttggca
      901 accattgatc agcatggttt ttctgctgaa gaactcgatg atgtcaaatc tacccgcctc
      961 acctggctga aaaatgcggt tgatcagcaa gctgaacgtg atttacgtat gctgaccagt
     1021 cgcctggcat ccagctcatt aaataatacg ccgttcttgt cgccggaaga gacatatcaa
     1081 ctttcgaaac gtctgtggca gcaaattacc gtgcaaagtc tggcggaaaa atggcagcag
     1141 ttaagaaaga accaggacgc attttgggag caaatggtaa acaatgagct tgccgccaaa
     1201 aaagcattgt ctcctgcggc tatcctggcg ctggaaaaag agtacgccaa caaaaagctg
     1261 gcggcttaca tcttcccagg cagaaattta tcgttaacag tagacgctga cccacaggcg
     1321 gaaattagca gcaaagaaac gctggctgag aatctgacat cattaacact ttccaatggt
     1381 gccaaggtta ttctggcaaa atccgcgggt gaagagcaaa agctacaaat tattgccgta
     1441 tcgaataaag gcgatttaag tttccctgcg cagcaaaaat cacttatcgc gctggcaaat
     1501 aaagcagtta gcggaagcgg cgttggcgaa ctctcctctt ccagcctgaa acgctggagt
     1561 gcggaaaact cggtaaccat gagcagtaaa gtcagtggca tgaatacgtt gctctccgtt
     1621 agcgcgcgga ctaataaccc tgaacctggt ttccagttga ttaaccagcg aatcacccac
     1681 agcacgatta acgacaatat ttgggcatcg ctacaaaatg ctcaaattca ggcgttgaaa
     1741 acgctcgacc agcgtccagc ggagaaattc gcccagctga tgtatgagac gcgctatgct
     1801 gatgaccgca cgaaattact gcaagaaaat cagattgcac agtttactgc cgcagatgcg
     1861 ctggctgccg atcgccagtt gttttcatct ccagcggata tcacgtttgt cattgtcggt
     1921 aatgtcgcag aagacaaact cgtggcgtta attacgcgtt acttaggctc aatcaaacac
     1981 tctgattcgc cattagccgc aggtaaacca ttaactcgcg cgacggacaa cgcatcggtt
     2041 actgtaaaag aacaaaatga acctgtggca caggtttcac agtggaagcg ttatgattcc
     2101 cggacacctg ttaatctggc gacgcgtatg gcgctcgatg cttttaacgt cgcactggca
     2161 aaagatctac gcgttaatat tcgtgaacag gcatctgggg catacagcgt ttcttctcgc
     2221 ctctcggttg atcctcaggc taaagatatc agtcatttgc tggcttttac ttgtcaacca
     2281 gaacgacatg atgaacagtt aacgttagcg aatgaagtga tggttaagcg tttgactaag
     2341 gggatcagtg agcaagaact gaacgaatac cagcaaaacg ttcagcgcag cctcgatatc
     2401 caacagcgta gcgttcaaca attagcgaac actattataa atagtcttat tcaatatgac
     2461 gatcctgcag catggactga gcaggagcaa tt
//
LOCUS       DAEBXN010000273         2463 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.277, whole genome shotgun sequence.
ACCESSION   DAEBXN010000273 DAEBXN010000000
VERSION     DAEBXN010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.277"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(746..2350)
                     /locus_tag="KJL99_004449"
     CDS             complement(746..2350)
                     /locus_tag="KJL99_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBD4438913.1"
                     /translation="MIQLSSSHKLPAVYYLYQRNGVYYFRLRVRQSNNDRMTSISLRT
                     KDRRTAMAYSRHIKAALKAIHADRPNATYEEMREHLKDIAECELSMGRSDLFEPDMRD
                     IYRDQYGELGESLTDALASEPLSIDQHRYINEALKVLKACMRRIEAGDSQPLIDYVDL
                     FNDIDRQDNQADSVSLSVNAPEVKPEVTPSITIASLFEQYEAENYQNWKPATLRENKA
                     SHAALIEIFDHLGLNADANRADMLRVRDVLQQLPRNRKQRFKDVPLADLLSREDKTDC
                     LDVVTINNKYLIKMAAVFRWAVRNDLIKKNMTEGLELKVPQRKASGARNAFSTEQVGQ
                     LLVAAKAYSQKTSGKPYHYYVTALAAITGARLNEIAQLQVKDVRTTEAGTVYIHINED
                     DSSLPGKSIKNAHSDRCVPLVDGAYGFILADFMALVETRRGADGDDAMVFDGLRLMKN
                     GYGEQVSKWFNRTLLPKVLVDRSGLAFHSFRHTVAAQLKQHGVELAYAQAIMGHSSGS
                     ITYDRYAKEVEVDRLVNVMADVYKET"
BASE COUNT          648 a          576 c          526 g          713 t
ORIGIN      
        1 aagacctata ggtttggtga ttgattatta taaatatcat caccccttaa ggtattccct
       61 tagagttaat ctttaattgg tctttaatta aatcttttat ttaatactta aagttaatct
      121 ctaattgtgt ctttaagtta actttaagta atactataag ttaatcttta attgtgtctt
      181 taagttaact ttaagttaag cccctgtagg gactagagac cataattaag atgattgtgt
      241 cttattgcta ttcacttatc gactgaccca gagcacacag gaagcattca ctttaagtag
      301 aacgcttggt gtctgtaatt ggtcatcccg cccctgaaag gatgcgataa gttacggtta
      361 gttgtcctag aagagaaaac tttttgtgtc ccttctccct atagtgagtc gtattaatta
      421 tccttgacaa gtagagaaaa ccatgagtca aaaacatgac cacacaggtc tgtagtaagt
      481 gtcatcctag aagagaaaac tttttgtgtc ccttctccct atagtgagtc gtattaatta
      541 ccccttgaca agcagagaaa actatgagtc aaaaacatga ccgcacaggt ctgtagtaag
      601 tgtcatccta gaagagaaaa ctttttgtgt cccttctccc tatagtgagt cgtattaatt
      661 atcccttgac aagcagagaa aactatgagt caaaaacatg accacacagg tctgtagtaa
      721 gtgtcatcct agactatggt ctcctctagg tctccttata aacatcagcc atcacattga
      781 ccagcctatc aacctcgacc tctttagcat aacggtcgta cgtaatagac cctgaactat
      841 gccccatgat cgcctgtgca tacgccaact cgaccccatg ttgttttaac tgggccgcaa
      901 ctgtatgtct aaacgagtga aacgctaagc cgctacggtc tacgaggact ttaggcaaca
      961 acgtccgatt aaaccattta ctcacctgct caccgtaacc gttcttcatg agacgcagac
     1021 catcgaacac catagcgtca tcaccgtcag cgcctctacg ggtctctaca agcgccataa
     1081 agtcagccaa tataaaacca taggccccat cgaccagagg cacacagcgg tcactatgag
     1141 cgttcttgat gctcttaccg ggtagactac tatcgtcttc attgatatga atataaacgg
     1201 ttcccgcctc agtggtcctg acgtctttga cctgtagttg ggctatttca ttgagtctcg
     1261 ccccagtgat agcagccaag gcggtaacat aataatgata cggcttacca gaggtcttct
     1321 gagagtatgc cttagctgcg accagtagtt gccccacctg ctccgtagag aaggcgttac
     1381 gggcccctga ggctttacgc tgagggactt taagctccag accctcagtc atgttctttt
     1441 taatcaagtc attgcgcaca gcccacctaa agacagcagc catcttaata agatacttgt
     1501 tgttgatggt aacgacatcc agacaatctg tcttatcttc acggctcaac aggtcagcaa
     1561 gtgggacatc cttgaagcgc tgcttacggt tcctaggtaa ctgctggagc acatcacgga
     1621 ccctaagcat gtcagccctg ttagcgtctg cattgagacc cagatggtcg aatatttcga
     1681 ttaacgcagc gtgtgacgcc ttgttttccc ttaaggttgc aggcttccag ttctgatagt
     1741 tctccgcttc gtattgttca aagaggctcg ctatggtaat gctaggcgta acttctggtt
     1801 ttacttctgg agcattaaca gatagggaca cagagtctgc ttggttatct tgcctatcaa
     1861 tatcattaaa aagatcgacg taatctatca gaggctgact gtctcccgct tcgatacgcc
     1921 tcatacaggc ttttagcacc ttcagggcct cattgatgta acgatgctgg tcaatgctta
     1981 gcggctcact agccagagca tcggtaaggc tctcaccaag ttctccgtat tggtcccggt
     2041 agatatccct catgtcaggc tcaaagagat ctgaacggcc catagacagt tcacactcag
     2101 cgatgtcctt aaggtgttcc ctcatttcct cataggtagc gttgggtctg tctgcgtgta
     2161 ttgccttgag tgctgccttt atgtgccttg agtaagccat agcggtgcgt ctatccttgg
     2221 tccgtaatga tattgaggtc attctatcat tgtttgattg tctgacccta agacggaagt
     2281 agtagacgcc gttacgctgg tagagatagt agactgctgg aagtttgtga ctactggata
     2341 gctgaatcat atgtctgcat gccctgagag ggttacacag tgggttacag taagtaactc
     2401 tgcaaggagg cttaaagccg tatcccttag gattcagtag gttggagtac tttaagaaag
     2461 gtc
//
LOCUS       DAEBXN010000274         2436 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.278, whole genome shotgun sequence.
ACCESSION   DAEBXN010000274 DAEBXN010000000
VERSION     DAEBXN010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.278"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..360
                     /locus_tag="KJL99_004450"
     CDS             <1..360
                     /locus_tag="KJL99_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HBD4438914.1"
                     /translation="PPQEETVVSIAVDPESPAQYLQRQKPQREEMPVYTRWVKTQKCM
                     TCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHNELHAGVKDFEEKHGSQLLLL
                     IRFLMHARNSGVLKWKA"
     gene            361..726
                     /locus_tag="KJL99_004451"
     CDS             361..726
                     /locus_tag="KJL99_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBD4438915.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVR
                     GQLVPGGRLGIKITELGCA"
     gene            723..1406
                     /locus_tag="KJL99_004452"
     CDS             723..1406
                     /locus_tag="KJL99_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HBD4438916.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGTSIPLVSPVEFSTSSWRRAVLSLEEHYK
                     AWLLWCYSGSICWEYQIAITQWAWNEFNAQSGTRKIAGKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTRHWVAMKHIFHRLDSEALLFVMRTRSKKRR
                     HFQSKVLQK"
     gene            1605..1680
                     /locus_tag="KJL99_004453"
     tRNA            1605..1680
                     /locus_tag="KJL99_004453"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1638..1640,aa:Ile,seq:cat)
     gene            1682..1758
                     /locus_tag="KJL99_004454"
     tRNA            1682..1758
                     /locus_tag="KJL99_004454"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1716..1718,aa:Lys,seq:ttt)
     gene            1761..1836
                     /locus_tag="KJL99_004455"
     tRNA            1761..1836
                     /locus_tag="KJL99_004455"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1794..1796,aa:Thr,seq:tgt)
     gene            1840..1914
                     /locus_tag="KJL99_004456"
     tRNA            1840..1914
                     /locus_tag="KJL99_004456"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1873..1875,aa:Gly,seq:tcc)
     gene            2207..2422
                     /locus_tag="KJL99_004457"
     CDS             2207..2422
                     /locus_tag="KJL99_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HBD4438917.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LVLGFLTYLTNLYFKIREDKRKAARGE"
BASE COUNT          640 a          520 c          653 g          623 t
ORIGIN      
        1 ccaccacagg aagagacggt ggtcagcatt gccgttgatc cggaatctcc ggctcagtat
       61 ctccagcgcc agaaaccaca acgggaagag atgcctgtat acacgcgctg ggtaaaaacg
      121 cagaaatgca tgacgtgtgg caatcaggca gatgatccgc accacatcat tggtcatgga
      181 ctgggaggga tgggaacaaa ggctgacgat ttgtttgtta ttccgctgtg tcgtaaatgc
      241 cataacgaac tacacgccgg ggtaaaagat tttgaagaaa aacacggtag tcagttgttg
      301 ttgctgattc gttttttaat gcacgcgaga aattcgggtg ttctgaagtg gaaagcataa
      361 atgaccgaac gcatagaatt tgttttgcct tacccgccaa cggtgaacac ctactggcga
      421 cgtcgtggca gcacatattt tgtatcaaaa gccggtgagc gttatcgccg tgatgtggcg
      481 ctaattgttc gccagcagcg actgaaatta aacctgtccg gaaggctggc gataaagatt
      541 attgcagagc caccggataa gcgccgtcgt gacctggaca atatcctgaa ggcaccgctg
      601 gatgcgctga cgcatgctgg actacttata gacgacgagc agtttgatga aatcaatatt
      661 gtgcgcggtc agctcgttcc tggtgggcgg ctggggataa aaatcacaga actggggtgc
      721 gcatgaataa ccagtattta cagtttgtgc gtgagcagct cattatcgcc accgctgatt
      781 tgagtggggc aacaaaaggt cagcttgaag cctggcaaga gaatgccatg ttcgatacag
      841 ggcgttacag gcgtaaaaaa atccggtacc gcgatgaagt gactggaaaa atgataacgc
      901 gggataatcc accaatcccg ggaaagcaat cgctggcgaa ggggacgtca attcctctgg
      961 tcagtccggt tgagttttcg acatcatcgt ggcggcgggc tgttctgtct cttgaagagc
     1021 actataaagc ctggttgttg tggtgttaca gcgggagtat ttgttgggaa tatcagatcg
     1081 cgataacaca gtgggcgtgg aatgaattta atgctcaatc cggtaccaga aaaattgcag
     1141 ggaaaacgca ggaacgcctg aaaaaattaa tctggctggc ggcgcaggca gtaaaagcag
     1201 aactttttgg tggggaaggt tatgaatacc aggagctggc attactggcg ggagtgacaa
     1261 ctaaaaactg gtccaaaaca tttactcgtc actgggttgc aatgaaacac atttttcacc
     1321 gactggatag tgaggcttta ttgtttgtaa tgagaacacg ttcaaaaaaa agacggcatt
     1381 ttcaaagcaa agtgttgcaa aagtagattg aaaggcatat attcatgcaa atctgatact
     1441 ttgccgattt tgtacgtgat ggcaaaagca aacaaaaccc gcccacaagc gggttttttg
     1501 tgccacttat ctcggataga catggtgaat gcgctggtgg aggagataag ggtgattttt
     1561 gaatgcttgc aacattgatt tcgtaacgtt attatcctgc gcccggccct ttagctcagt
     1621 ggtgagagcg agcgactcat aatcgccagg tcgctggttc aaatccagca agggccacca
     1681 accgccacta gctcatcagg aaagagcgtc aaccctttaa gttgagtgtg cgaggttcga
     1741 gtccccggtg gcggtccagt gccgacttgg ctcagtaggt agagcaactg acttgtaatc
     1801 agtaggtcac cagttcgatt ccggtagtcg gcaccatatg cgggcatcgt ataatggcta
     1861 ttacctcagc cttccaagct gatgatgcgg gttcgattcc cgctgcccgc tccagtcaga
     1921 gtctttcagt ctgcgatgat gggaaatccc ggagtgactg aaagacgttt aagttatgaa
     1981 tgatcgcttt tttttgcaaa attgctgtgc agaaatacta accttcgggc aggcgatcat
     2041 tcataagcac tctgctttta ttccgattaa ctgtgagtgg tttgttggat agagtgcttt
     2101 ccttattgta tatatcgttt cgcccgcttt tgcggttttt tcttttcaaa tccctttcat
     2161 ttctcagtgt aaaactacgc catccgttat ttgcggaggt gaggctatga aatccatgga
     2221 caaaatttca acgggcattg cctacggcac ctccgcaggc agtgctggct actggttttt
     2281 acagtggctt gatcaggtca gtccgtcaca gtgggctgcg attggtgtac tggggagtct
     2341 ggttctgggc ttcctgactt atctgacaaa tctgtacttc aaaatcagag aagacaagcg
     2401 taaggctgcg agaggtgaat aatgtcgcca tcatta
//
LOCUS       DAEBXN010000275         2420 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.279, whole genome shotgun sequence.
ACCESSION   DAEBXN010000275 DAEBXN010000000
VERSION     DAEBXN010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.279"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..201
                     /locus_tag="KJL99_004458"
     CDS             <1..201
                     /locus_tag="KJL99_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438918.1"
                     /translation="KLKMMLFGASLIMVVGCSSKENALCHPQPKPPAPPAWAMMPPSN
                     SLQLLDETFSVSGTESSATRQH"
     gene            complement(334..846)
                     /locus_tag="KJL99_004459"
     CDS             complement(334..846)
                     /locus_tag="KJL99_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HBD4438919.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            1041..1805
                     /locus_tag="KJL99_004460"
     CDS             1041..1805
                     /locus_tag="KJL99_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438920.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLDWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDAESGFT"
     gene            1756..>2420
                     /locus_tag="KJL99_004461"
     CDS             1756..>2420
                     /locus_tag="KJL99_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HBD4438921.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDMLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAA"
BASE COUNT          683 a          540 c          614 g          583 t
ORIGIN      
        1 aagctgaaaa tgatgctctt cggcgcaagc ttgataatgg tggtcgggtg ctcgtcaaag
       61 gaaaatgccc tgtgccatcc tcagccgaaa cctccagcgc ctccggcatg ggcaatgatg
      121 ccaccgtcga actctctcca gttgctggac gaaacgttct cggtatccgg gacggaatca
      181 tcagcgacca gacagcattg agaatgcttc aggagtacat caggatccaa tgccttgggg
      241 ggtagcggta attttactca ttatccttca aatcagattc tgttgtcaga ggaatggggg
      301 aagctggatt cgcaagagct ttttatgggc agtttagcta aaaaaaggca acacgaagct
      361 aaaactacca acataaagtg ctgaccagct agcagctgaa atcagactca ctgtatacac
      421 tttcactgga tgaagttgca acattcctgc caccaaagga gcaatgtaac gcagcacggc
      481 gataaagcgc gaagtgaaaa gtattgacac agagttattt tgcaactgaa ggcgaacccg
      541 ttctattgta ttggaacggc tcgatattat acatgcaagt tgagggatat gcccaattat
      601 tataccaaga tggtaattaa cgatagtacc gcaccatgcg ccactcatga ccgtgattcc
      661 agcttcccat ggtgaaagag tcgtcttgct gactgtaatt acagtggcca tcataattga
      721 tgctggtggc aatatcgccg aaatgagtaa agtggatttt gtgaacgcta ttataaaaag
      781 aagcccccat aggcgatatg gatgaagtac aaaatagttc atcaaagcat ttatccactc
      841 catcaatgtc tttccttctg tgaatttttc tttgctattt aaccgtgata cgacttaatt
      901 taggcttaaa caccttacat ttacaaggta ctcctgtggg gggccctgcc acggagcgtc
      961 gcaggagtga gtgatgagga agaattaaag caacctattg tgacaggcag cgataacagt
     1021 aaatcacagg aagcataatc atggcaaaac cggactggga ggccatcgaa tcggcatacc
     1081 gggccggagt ccttagcctc cgtgatatag gcgagaaata cggcgttaca gaaggggcta
     1141 tcaggaagag ggccaaaaag cttgattggg cacgcagtgg cggtacgcag gtttgcaaaa
     1201 atggtacgca aaaaaggaaa gtgcgtacca gcagaaagcc tgccattact ggccttacac
     1261 aaaaaagtac gcaactaaaa acagaatcta caccggatac gaaaccgata cgcggaatgc
     1321 gtaccgatcc cccgactaac ccattccaac ccggtaacca gcaggcatta aaacacggtg
     1381 gttatgcccg tcgccttctg ctcaaagatg aggtgataga ggacgctaaa gcgttgacgc
     1441 tcgaggacga attatttcgc cttcgtgcta acaaccttgt cgctgcagag aatattggtc
     1501 ggtggttggt gtcgctggaa gatgctaatg gggaccagga aaggaagatg ctgattgaaa
     1561 atatcagcgc cgccgagaaa gcaatgatgc gcaatacagt tcgtattgag tccatcgttg
     1621 gcacgcttgc gacggtagga aaaatatttg ctgatacagc ctaccgcaag gccgccactg
     1681 ataaggtgtc tctggaggct gatcgtcttc gccgtgatgc aggtattgat gatggcaatg
     1741 gagagcgtga cctcaatgac ttctactctg acatccaaac cgacgctgaa tccggcttta
     1801 cgtagtttct ggactatgcg ggcacgtaac aaagtgcttt atggtggtcg gtcatcgtca
     1861 aaatcatggg atgccgctgg cattgccata tttctgtcga ataaatacac cctgcgtttt
     1921 tgttgtgccc gtcagatcca gaataaaatc gaagagtcgg tgtataccct gctcaaaatt
     1981 cagatagaca ggtttggtct gcggcaccgt ttccgtattc tgaacaacaa aatcattaac
     2041 cgggttactg gctcggaatt tgttttttat ggattatggc gcaacatcga agaaattaag
     2101 tcactggagg ggatcgatat gttgtggctg gaagaagccc acgcactgac ggaataccag
     2161 tggaaaattc tggagccaac gatccgtaaa gagggttcgg aatgctggtt catattcaac
     2221 cccggacttg ttactgattt cgtctggcgc aacttcgttg ttgatccgcc cgaaggcact
     2281 ctcatccgca aaattaacta tgacgaaaat ccgtttctgt ctgacaccat gcttaaggtt
     2341 atcgacgcgg cgcgacgccg tgatccggat ggttttaaac atgtgtatga gggcgttccg
     2401 gagtctgatg atgatgcggc
//
LOCUS       DAEBXN010000276         2349 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.280, whole genome shotgun sequence.
ACCESSION   DAEBXN010000276 DAEBXN010000000
VERSION     DAEBXN010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.280"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..377
                     /locus_tag="KJL99_004462"
     CDS             <1..377
                     /locus_tag="KJL99_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBD4438922.1"
                     /translation="RRPNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQ
                     YRKGGLLPSGKNMPALLPVTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLR
                     LKGKLTPALLQILIREIKGSSH"
     gene            377..724
                     /gene="tnpB"
                     /locus_tag="KJL99_004463"
     CDS             377..724
                     /gene="tnpB"
                     /locus_tag="KJL99_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HBD4438923.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLVSKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            744..1973
                     /locus_tag="KJL99_004464"
                     /pseudo
     CDS             744..1973
                     /locus_tag="KJL99_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            1977..2136
                     /locus_tag="KJL99_004465"
                     /pseudo
     CDS             1977..2136
                     /locus_tag="KJL99_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          577 a          614 c          640 g          518 t
ORIGIN      
        1 accggcggcc taattttcct tatgaattta aaatcgcttt agtggagcag tcactgcagc
       61 ccggagcctg tgtggcgcag atcgcccgtg aaaacggaat caacgataac ctgctcttca
      121 actggcgcca tcaataccgg aaaggtggcc tgctgccttc cggaaaaaat atgcctgcac
      181 tgcttcccgt gacgttaacg ccggagccgg ataataaaat cccggctccg gcacaggaac
      241 cagagcagat aaatacaccg tccgacagtc tgtgctgtga gctggttctg ccggccggaa
      301 ctctcaggct taaaggtaaa ctgacgccgg cgttattaca gatacttatc cgcgaaataa
      361 aagggagcag ccactgatga tatctttccc tgcaggttcg cgtatctggc tggttgcagg
      421 tatcaccgat atgcgaaatg gctttaacgg cctggtctca aaagttcaga acgtcctgaa
      481 ggatgacccg ttctccgggc atctgttcat cttccgcgga cgccggggtg accagataaa
      541 agtgttgtgg gctgacagtg acggactgtg cctctttacc agacgcctgg agcggggccg
      601 cttcgtctgg ccggtcaccc gtgacggcaa ggtgcacctt actccggccc agttatccat
      661 gcttcttgag ggcatcgact ggaaacaccc gaaacgaacg gaacgcgctg gcatccgcat
      721 ataacccgtt gtaaagtgag gatatggaca cctcacttgc tcatgagaat gcccgcctgc
      781 gggcactgtt gcagacgcaa caggacacca tccgccagat ggcgaaatac aaccgcctgc
      841 tctcacagcg ggtggcggct tatgcttccg aaatcaaccg gctgaaggcg ctggttgcga
      901 aactgcagcg catgcagttc ggtaaaagct cagaaaaact tcgcgcaaaa accgaacggc
      961 agatactgga agcacaggag cgaatcagcg cacttcagga agaaatggcg gaaacgctgg
     1021 gtgagcaata tgacccggta ctgccatctc ccctgcgcca gtcttcagcc cgtaaaccgt
     1081 tacctgcctc acttccccgt gaaacccggg tcatccgtcc ggaagaggaa tgctgcccgg
     1141 cctgtggtgg cgaactcagt tctctgggat gtgatgtgtc agagcagctg gagcttatca
     1201 gcagcgcctt taaggttatc gaaacacaac gtccgaaact ggcctgttgc cggtgtgacc
     1261 atatcgtgca ggcaccggtg ccttcaaaac ccattgcacg cagttatgcc ggagcggggc
     1321 ttctggccca tgttgttgct ggaaaatatg cagactatct gccgttatac cgccagtcag
     1381 aaatataccg tcgtcagggc gtggagctga gccgtgccac actggggcgc tggacaggtg
     1441 ctgttgctga actgctggaa ccgctgtatg acatcctgcg ccagtatgtg ctgatgcccg
     1501 gtaaagtcca tgccgatgat atccccgttc cggtccagga gccgggcagc ggtaaaacaa
     1561 ggactgcccg gctgtgggtc tacgttcgtg atgaccgtaa cgccggttcg gaaatgcctc
     1621 cggcggtctg gttcgcgtac tcaccggacc ggaaaggtat ccatccacag aatcatctgg
     1681 ccggttacag cggagtgctt caggccgatg cttacggtgg ttaccgggcg ttgtacgaat
     1741 ccggcagaat aacggaagcc gcatgtatgg ctcatgtccg gagaaaaatc cacgatgtgc
     1801 atgcaagagt gccaaccgac atcaccacgg aagccctgca gcgtatcggt gaactgtatg
     1861 ccatcgaggc agaagtccgg ggatgttcag cagaacagcg tctggcggca agaaaagcca
     1921 gagccgcgcc actgatgcag tcactgtatg actggataca gcaacagatg aaaattcatt
     1981 cgctgaaaat ggaatgtctc cacggagaac actattatcc atcgggaaat agtgcgggca
     2041 acagtgttta gttatatcga gtgcgattac aatcgctggc gacggcaccg ttggtgtggt
     2101 ggtctcagtc cggaacaatt tgaaaaccag agcctcactt acgactgtgt ccatattatg
     2161 tgggtaggat caataaagac gttttatatg tgtctgcttg cagccagaaa atttataccc
     2221 tgcaggtatc ataaacatgc ttgacaactt aattagggcc ccctcttcct tttatggtgt
     2281 aattgtttga ttgaatactt ccggggattt cagtatgaaa tgaagtatat ttaatatact
     2341 ttacaaaaa
//
LOCUS       DAEBXN010000277         2325 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.282, whole genome shotgun sequence.
ACCESSION   DAEBXN010000277 DAEBXN010000000
VERSION     DAEBXN010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.282"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            61..282
                     /gene="kilR"
                     /locus_tag="KJL99_004466"
     CDS             61..282
                     /gene="kilR"
                     /locus_tag="KJL99_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="killing protein KilR"
                     /protein_id="HBD4438924.1"
                     /translation="MIAHHFGTDEIPRQCVTPGDYVLHEGRTYIASANNIKKRKLYIR
                     SLTTKTCISDCMIKVFLGRDGLPVKAESW"
     gene            276..452
                     /locus_tag="KJL99_004467"
     CDS             276..452
                     /locus_tag="KJL99_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438925.1"
                     /translation="MVMTKKIKCAYHLCNKEIEESKIITRPLHFMRGVIPTTEMKKYC
                     SEICAEKDQMAHEL"
     gene            527..802
                     /locus_tag="KJL99_004468"
     CDS             527..802
                     /locus_tag="KJL99_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438926.1"
                     /translation="MITSYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLNDGVKKMCLVHIGKNLPAEKKAEFLATLIAMKLKGEI"
     gene            902..>2325
                     /locus_tag="KJL99_004469"
     CDS             902..>2325
                     /locus_tag="KJL99_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HBD4438927.1"
                     /translation="MNTDKQVYPLYYEAKNDKVRKRLGIKGGFYWAEAKKLSIAISRG
                     AVAIDDAGYDEDDFKKPVRVNLPVVDDLPPEGVFDTEFCNRYEKGGEDGITMVFIASS
                     PSVQDKPASTDNTNVNGEDMTEIEENMLLPVSGQELPIRWLAQHGSEKPVTHVSRDEL
                     QALHIARDEELPAVTALAVSHKTSLLDPLEIRDLHKLVRDTDRVFPNPGNSSLGLMTA
                     FFEAYMDADYTDRGLLTKEWMKGNRVSRITRTASGANAGGGNLTDRGEGFVHDLTSLA
                     RDVATGVLARSMDVDIYNLHPAHAKRVEEIIAENKPPFSVFRDKFITMPGGMDYSRAI
                     VVASVKEAPIGIEVIPAHVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMPQRVTEE
                     GKQDDEEKTQPSGAMADEQATAETMEPDATEHHQNTQPLDAQSQVNSVDEKYQELRAE
                     LHEARKNIPPKNPVDADKLLAALR"
BASE COUNT          719 a          531 c          529 g          546 t
ORIGIN      
        1 taagtattaa ataactttcc tgagatttcc tttgtaagac tgtttattaa ggaggcttcc
       61 atgattgctc atcacttcgg aactgatgaa ataccacgtc agtgtgtgac tcctggcgat
      121 tatgttcttc atgaaggtcg gacatatatc gcctcggcaa acaatattaa aaagcgaaaa
      181 ctttatattc gtagcctgac tacaaaaaca tgcatttctg actgcatgat taaagtcttc
      241 ctcggtcgtg atggtttacc tgtaaaggcg gagtcatggt aatgactaag aaaataaaat
      301 gtgcttatca cctttgcaat aaagaaattg aagaaagcaa aatcattaca agaccacttc
      361 atttcatgcg tggagttata ccaacgacgg aaatgaaaaa atattgtagt gaaatctgtg
      421 ccgaaaaaga ccagatggca cacgaacttt aattaactga ctatccgaaa ctgaatttat
      481 gccagcaatg gcagggattc gctcaacctt aattaaggag aaaaacatga ttaccagtta
      541 tgaagccact gttgtaacta ctgatgacat tgttcacgag gttaatctgg aaggaaagcg
      601 tattggctac gtgattaaga cagaaaataa agaaacccca ttcactgtgg ttgatatcga
      661 cggtccatca ggcaacgtta aaacacttaa cgatggtgta aaaaaaatgt gtctggtgca
      721 cataggaaag aatctgcccg cagaaaaaaa agccgaattt ctggcaactc tgattgcaat
      781 gaaattaaaa ggtgaaatct gaaaaaaaga aagcctgcac actgtgcagg cctgaatgaa
      841 gaacctggga catttattca tcactcgcag taattttaat ctgagttgag gttaaaaaac
      901 aatgaacacc gataaacaag tttacccact gtattacgaa gcaaaaaatg acaaagtaag
      961 aaaacgtctc ggtattaaag gcggttttta ctgggctgaa gcgaaaaaat tatccattgc
     1021 catctcccgt ggtgctgttg cgattgacga tgcaggctac gatgaagatg actttaaaaa
     1081 acctgttcgc gtcaatttgc ccgttgttga tgacctcccg ccagaaggcg tatttgatac
     1141 ggaattctgc aaccgttacg aaaaaggcgg ggaagatggc atcacaatgg tatttatcgc
     1201 atcttctccc tctgttcagg acaaaccggc cagcactgac aataccaacg tcaacggcga
     1261 agacatgaca gaaattgagg agaacatgct cctgccagtt tccggtcagg aactgcccat
     1321 tcgctggctt gcgcaacacg gcagcgaaaa accagtaacg cacgtttcac gcgacgaact
     1381 ccaggcatta cacattgctc gggatgaaga actaccagct gttactgccc tggctgtttc
     1441 ccacaaaacc agcctgctcg atccgctgga aattcgcgat ctccacaaac tggtgcgtga
     1501 tactgacaga gttttcccta atccaggcaa ttcaagcctg gggctgatga ctgctttttt
     1561 cgaagcatac atggacgcag actacaccga tcgcggtctg ctgacaaaag agtggatgaa
     1621 aggaaatcgt gtttcacgca tcacgcgcac ggcttccggc gctaatgctg gcggcgggaa
     1681 cctcaccgat cgcggcgaag gtttcgttca cgatctgacg tcactggcgc gcgacgtagc
     1741 cactggcgta ctggctcgtt caatggacgt ggacatttat aaccttcatc cggcacacgc
     1801 taaacgtgtc gaggaaatta tcgctgaaaa taaaccgccc ttttctgttt tccgcgacaa
     1861 attcatcacc atgcctggcg ggatggatta ttcccgcgcc atcgtggttg cgtcagtgaa
     1921 agaagcaccg attggtatcg aggtcatccc cgctcacgtc actgaatatc tgaacaaggt
     1981 actgactgaa accgatcatg ccaaccctga tccggaaatc gtggatattg cctgcggtcg
     2041 ctcctctgcc ccgatgccgc aacgtgtaac agaagaagga aaacaggatg atgaagaaaa
     2101 aacgcagcca tctggcgcaa tggcagatga acaggcaacg gctgaaacaa tggaaccgga
     2161 tgcaactgaa catcatcaga acacgcagcc gctggatgct cagtcacagg taaattctgt
     2221 tgatgagaaa tatcaggaac tacgggcaga actccatgaa gcccggaaaa acattccgcc
     2281 caaaaatcct gtcgatgcag acaaattact ggctgccttg aggta
//
LOCUS       DAEBXN010000278         2274 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.283, whole genome shotgun sequence.
ACCESSION   DAEBXN010000278 DAEBXN010000000
VERSION     DAEBXN010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.283"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            150..911
                     /locus_tag="KJL99_004470"
     CDS             150..911
                     /locus_tag="KJL99_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HBD4438928.1"
                     /translation="MKNFNVLTKVVFCLSFLSSAFPTYAADDVKVTQNTSVFKLKLSA
                     TRLIYEQGSDGAAITVTNPQDWPMLVQSKVFEEDKVTAAPFIVTPPLFRLNPGQQNKM
                     WVVQTAPALQDREKLHWLCATGITPKKSDVWAEKDKEKIAENVNLNIQVNVHTCIKLI
                     TRPSGIEGKMVNASEKLSWKKQGDKIVAVNQSPFYINLSSLQVGGSKIEKLDYIAPFS
                     QHEFVMPKTSGQDIKWSVITDEGGESRLFISHLQS"
     gene            955..2034
                     /locus_tag="KJL99_004471"
     CDS             955..2034
                     /locus_tag="KJL99_004471"
                     /inference="COORDINATES: protein motif:HMM:NF025320.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005093492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBD4438929.1"
                     /translation="MFYRLSLILIMALLAGQLSAQEWSFDSSQLEGNVSADTVAMFNQ
                     GEQLPGNYRVEIYLNGEKVDVGEFPFHRPESPEEKELVPCLTVDDLIHYGIKIDKSSS
                     DTDNKKNQCFKWNSIEGLKVNYDFDSQRVQITVPQLYLQDKKSSLAPVSLWNEGVAAF
                     RMVYQTNIDISKQNDNQSTTRNSRYGRFTPGFNLGAWRFRSSVTWSKELGQSERWQRG
                     YMWFERGINAIKSRLTLGESYTSSEVFDSIPFRGGMLATDDAMTPPEDSYYTPVVHGI
                     AQSEAQVIIKQNGQIIFTRSVPPGPFALDNLPTLAVGGELDVTVRESNGEEQYFSVPF
                     QTPAIALHEGYFKYSVMGGNIKKKV"
     gene            2031..>2274
                     /locus_tag="KJL99_004472"
     CDS             2031..>2274
                     /locus_tag="KJL99_004472"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HBD4438930.1"
                     /translation="MKSSPVSVGEITAMYGLPYGVTVYGGIQNATHFNAISTGIGISL
                     GLLGSLSTDITRSIANLYYGNKYRIRYSKSISDFGTQ"
BASE COUNT          670 a          403 c          545 g          656 t
ORIGIN      
        1 agaaataaca aaattaagaa aagccatatt tacgatatgg gcaacataaa tataatctaa
       61 aaaagagtaa ggcgttgtag gttggttccg cgttcattac cttgttagtg aacgcggatt
      121 tatcagttgt cgaaatggtt tttataataa tgaaaaattt taatgtacta accaaagtcg
      181 tattttgctt gtcgttttta tcaagcgcat ttcccaccta tgctgcggat gatgtcaaag
      241 tgactcaaaa cacttcagtt tttaaactga agcttagtgc aacgcgatta atctatgaac
      301 aagggtcgga tggcgcagcg ataactgtga caaatccgca ggattggccg atgcttgtgc
      361 agagtaaagt ctttgaagaa gataaagtaa ctgcagcgcc ctttattgtc actccgccac
      421 tgttcaggct taatccaggc cagcaaaata aaatgtgggt tgtgcagact gcgccagctt
      481 tacaagacag ggaaaaatta cactggctat gcgccaccgg cattacgcct aaaaagagtg
      541 atgtttgggc cgagaaagat aaagaaaaaa ttgctgaaaa tgtgaacctg aacatccagg
      601 ttaacgtaca tacctgcata aaacttatta ccaggccatc tggtattgaa ggcaagatgg
      661 ttaatgcgag tgagaagtta tcgtggaaaa agcagggtga taaaattgtc gcggtcaatc
      721 agtcgccgtt ctacatcaac ctttcttctt tacaggttgg cggtagcaag attgagaaac
      781 tagattatat tgcgccgttt tcgcaacatg agtttgttat gccaaaaact agcggccagg
      841 atattaaatg gtctgtgatt actgatgaag gtggtgagag tcgtttgttt atatctcatt
      901 tgcaatcata atgtattaca tttttattga atatttttat gccatgcata ttccatgttt
      961 tatcgactgt cattaatttt gataatggcc ttacttgcgg gtcagttaag tgcccaggag
     1021 tggtcatttg attcttcaca gttggaagga aacgttagtg cggacactgt ggcaatgttt
     1081 aatcagggag agcaactccc cggaaattat cgtgtggaaa tttatcttaa cggtgaaaaa
     1141 gttgatgttg gggagtttcc ctttcatcgt ccggaatccc ctgaagaaaa agagcttgtt
     1201 ccatgtttaa ccgttgatga ccttatccac tatggcatta aaattgataa aagcagcagt
     1261 gatactgaca ataaaaagaa tcagtgtttt aaatggaact ctattgaagg tttgaaagta
     1321 aattatgatt ttgatagcca gcgcgtacag ataaccgttc cacaactgta tctgcaagat
     1381 aaaaaaagta gtttagcacc agtgtcgtta tggaatgagg gtgtagctgc attccgcatg
     1441 gtgtatcaga caaatattga tatttccaaa caaaatgata atcaatctac cacgcgtaat
     1501 tctcgttatg gcaggttcac tccagggttt aatcttggtg cctggaggtt tcgtagttca
     1561 gtgacatgga gcaaggaact tggccagtca gaaagatggc aaaggggtta catgtggttt
     1621 gagcgaggca taaatgcgat taaaagccga ttaacgcttg gagaatctta tacgtcgtct
     1681 gaggtgtttg acagcatacc ttttcgcggc gggatgttag cgaccgacga tgcgatgact
     1741 ccgccggagg acagctacta cacacctgtg gtgcatggaa tagcacaaag tgaggcgcag
     1801 gttattatta aacaaaatgg gcaaattatt tttacccgtt ctgtgccgcc agggcctttt
     1861 gcgctggata atttaccaac gctggccgtt ggcggggagc ttgatgttac agtgagagag
     1921 agtaatggcg aggagcagta tttttccgtg ccatttcaaa cgccggccat cgctctgcat
     1981 gaaggatatt tcaaatatag tgtgatgggg gggaatataa agaagaaggt ataaaaagct
     2041 cccctgtctc cgtgggcgaa atcacggcga tgtacgggct tccttatggc gtcacggtgt
     2101 atggagggat tcaaaacgcc actcatttta atgcaatatc tacgggtatt ggcatttctc
     2161 tggggctgtt agggagttta tcgacagata ttacgcgttc gattgcgaat ttatattatg
     2221 ggaataaata tcgtattcgt tatagtaaat ccattagtga ttttggtact caat
//
LOCUS       DAEBXN010000279         2230 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.284, whole genome shotgun sequence.
ACCESSION   DAEBXN010000279 DAEBXN010000000
VERSION     DAEBXN010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.284"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            651..2159
                     /gene="ltrA"
                     /locus_tag="KJL99_004473"
     CDS             651..2159
                     /gene="ltrA"
                     /locus_tag="KJL99_004473"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBD4438931.1"
                     /translation="MQRKLATWAATDPSLRIQRLLRLITQPEWLAEAARITLSSKGAH
                     TPGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLGIPALR
                     DRIVQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDCGETRGRWVIEG
                     DLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGVPQGGVI
                     SPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKPAVAYCR
                     YADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVNDGFIFLGHRIIR
                     KRSRYGEMRVVSTIPQEKARNFAASLTALLSGNYSESKVDMAEQLNRKLKGWAMFYQF
                     VDFKAKVFSYIDRVVFWKLAHWLARKYRTGIASLMRWWCKSPKPGQSKTWVLFGKTNH
                     GKLSGEILYRLVGQGKKLFRWRLPEGNPYLRTETRNTYTSRFTEVAMAFASI"
BASE COUNT          572 a          548 c          657 g          453 t
ORIGIN      
        1 ggcatggatg cttaaatgaa ccgcgagtct gtctggaata ttgaaccggt aactcacgat
       61 gagaaaccca acaatcccac cgggtgtgac ggtggagaac ctgagcggca gtgacctgcg
      121 gcatgcccgc agggtgatgt aacccgctga caacggggat tgaggcgaga tcactaagcc
      181 gagatgatcc tcaaggttaa gtactgaaag gctgaagaac atgaacccgt taatccgcct
      241 ctgtgggttg aaaacgtcac cacggcctac gtgatctgac aggccgtgca ggaggaactg
      301 gcagtgatac gtaagcactg ccggtcgaag gtgttttgac atgtatgcga aacaccgggg
      361 cagcagcgtc tatcacactt gcgttgctga cttctgccaa cttgcggcaa gcaaggataa
      421 agagtgcgac gggcagcctc ctcagtatgc ctgagtccag gcaggtaaac cggggaaggt
      481 cagcgacgga tgttaagggg gcatggctcc gatgacgcgc tggctggcgg agcttccgta
      541 gtagtccgcg atggggaaag cccattacat ggcgaaggga agcagtttga atgtgtttgc
      601 gacgtgaatt aactgaccta acgaggtgaa gacctttgat aatcagcgaa atgcaacgca
      661 agcttgccac atgggcagcc accgatccgt ccctacggat tcaacggctg ctgcgtctga
      721 taacacaacc agaatggctg gctgaagcgg cgcggatcac gctttcatca aagggggccc
      781 atacccccgg cgttgatggc gtgaacaaaa caatgctaca ggccagactg gctgttgagc
      841 tgcaaatcct cagggatgaa ttactctcag gccactacca gcccttgccc gccagacggg
      901 tttacatccc taaaagcaac ggcaaactgc gaccactggg tatccccgcg ttgcgggatc
      961 gtattgttca gcgggccatg ctgatggcga tggagccgat atgggagagt gattttcata
     1021 cgctctcata tggcttccgg cctgagcgca gtgtccacca cgcgatccgc acggtgaaat
     1081 tacagctcac agactgtggt gaaacccggg gacgctgggt gattgaaggc gacctgtcca
     1141 gttacttcga caccgtacat catcgactgc tgatgaaggc tgtacgccgc aggatcagtg
     1201 acgcacgttt catgactctg ctgtggaaaa ccatcaaggc gggacatatc gatgtcggtc
     1261 tctttcgggc ggccagtgaa ggtgtgccac agggcggtgt tatatcgccg ctattatcga
     1321 acatcatgct gaatgagttc gatcaatacc tgcatgagcg ctacctgagc gggaaagcca
     1381 gaaaagatcg gtggtactgg aataacagta tccaacgggg ccgaagtacg gcggtcagag
     1441 aaaactggca gtggaaaccc gcggtggcgt actgccgcta tgccgatgat tttgtcctca
     1501 tcgtcaaagg caccaaagca caggcggaag ccatcaggga ggagtgtcgg ggtgtgctcg
     1561 aaggcagtct gaaactcagg ctgaacatgg ataagactaa aatcacccat gttaatgacg
     1621 gctttatctt tctggggcac aggatcattc gcaaacgcag tcgttatggc gagatgcgag
     1681 tggtctcaac gatcccgcag gagaaagcca gaaacttcgc cgcatcgctg acagcactgt
     1741 tatcaggcaa ctacagtgaa agcaaagtcg atatggctga acaactcaac cgaaaactga
     1801 aaggctgggc catgttctat cagttcgttg attttaaggc caaagtcttc agttatatcg
     1861 accgtgtcgt gttctggaag ctggctcact ggctggcccg caaataccgt acaggtatcg
     1921 cttccctgat gaggtggtgg tgtaaatcac cgaaaccggg tcagagcaaa acgtgggttt
     1981 tatttggtaa aaccaatcac ggcaagctca gcggcgaaat actgtaccgg ttggtggggc
     2041 aaggcaagaa gctgttccgc tggcggctac ccgaaggtaa tccctatctg aggacggaga
     2101 ccagaaacac gtatacatcg cgctttacag aagtggcaat ggcgttcgcc agcatttaaa
     2161 tggagagccg gatgcgctga aaggtgcacg tccggttcgg ggaggagagg cagggaaata
     2221 gtccgactac
//
LOCUS       DAEBXN010000280         2227 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.285, whole genome shotgun sequence.
ACCESSION   DAEBXN010000280 DAEBXN010000000
VERSION     DAEBXN010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.285"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..1224
                     /locus_tag="KJL99_004474"
     CDS             <1..1224
                     /locus_tag="KJL99_004474"
                     /inference="COORDINATES: protein motif:HMM:NF027197.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HBD4438932.1"
                     /translation="FPYGIFQQRNVLASWLATGCGVALYLALTARTRSLTLICLLSLY
                     PLSTALVLTQSRVGLLGLFCVIVLAALADRPRWRGRPLSGLLRLIFVASLLCWCVGIS
                     NYAMPGETTADFTHSSSNDQRIRILKGAISLIQQRPLTGWGLGSFEATFPQALEDVGV
                     TNIESDTATHPHNELLFVTAEGGIVALFGFLLLSGVWLWPCICRLKMTNNNAHHQCEK
                     WFIALMGLPLVIHMMTEYPLYTSVPHLMLLLLLFRIGMPENVMNQVRIPKTVRLGILP
                     VIVLIMTAALVILVAGFDAQRELTRAEADMNEGFIPRLPEHSWKYLTQAERLERDQHL
                     LIANKPGFVRDTESMSLFTVWGNKWLAIHNDADVSAAMIFIAHRRGDYLTAEKIRKRA
                     ARVFVNDERFTRGGN"
     gene            1224..1958
                     /locus_tag="KJL99_004475"
     CDS             1224..1958
                     /locus_tag="KJL99_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBD4438933.1"
                     /translation="MVNDLTYKKLESTNINCNILSPENTTWTNVTVIGLDLFAICGVC
                     SLLKRVGGVKLQVFTSLPTDMNVLTDSSIVIWVKMRHDGLPELAGHIVKVCRRYRLMR
                     QLVISDAIPEGMTNDIGPLSRVWLTHGSAKVDILLSSLRKVLNAHCLPGPLFIKRLGR
                     VQWRVLLLRAKGISTRKIAEICGIGLKTVSAHESAIRERLGTRNKSEYVWLVRNAQLI
                     QQAIPALGRDASRYERCNNMRTKQKV"
BASE COUNT          561 a          455 c          565 g          646 t
ORIGIN      
        1 tttccgtatg gcattttcca gcagcgaaat gttcttgcga gctggctggc aacaggttgc
       61 ggcgtagcgt tgtatctggc tttaacggcg cgtactcgtt ctctgacgtt gatatgtttg
      121 ttgtcgttat accctctgtc tacagcttta gtccttacac aaagccgtgt cggccttctt
      181 ggtttatttt gcgtcatcgt gctggccgct ctcgcggaca gaccccgctg gcgcggacgt
      241 ccccttagcg gtctgttgcg cttgatcttc gtcgcctcct tgctgtgctg gtgtgtcggt
      301 atctcaaact atgccatgcc aggggagaca acggctgact ttacgcattc atcatcgaat
      361 gaccagcgta tacgtattct gaaaggtgcc ataagtctta ttcaacagcg ccccttaacc
      421 ggttgggggt taggcagctt tgaagcgacc ttcccgcaag ccttagagga tgtgggcgtg
      481 acaaacatag agagtgatac ggctacgcat ccccataacg aactgctgtt cgtaacggct
      541 gaaggtggca tcgtcgcctt gtttggtttt ttgctgctat caggcgtttg gctgtggccg
      601 tgtatatgcc gcctgaaaat gacaaacaat aacgcgcacc atcaatgtga aaagtggttt
      661 attgccttaa tggggttgcc tttagtcatt catatgatga ccgaatatcc tctctatacg
      721 tctgttcccc atttgatgct gttgctgctc cttttccgca tcggaatgcc ggaaaacgtg
      781 atgaatcagg tacgtatacc taaaacagtc cggttgggta ttctgcctgt cattgtgctc
      841 attatgaccg ccgcgctggt catcctggta gcgggatttg atgcacagcg ggaactgacc
      901 agagctgaag ctgatatgaa cgaaggattt atccctcgcc ttccggaaca tagctggaaa
      961 tacctgacgc aggcagaacg tcttgagcgc gatcagcatc tgcttattgc gaataaaccg
     1021 gggttcgttc gcgatacgga aagcatgtct cttttcactg tgtggggaaa taaatggttg
     1081 gcaatccaca atgatgcaga cgtaagcgct gcaatgatat tcattgcgca caggcgcggt
     1141 gattatctga cagctgaaaa aataagaaaa cgcgcagctc gggtatttgt aaatgacgaa
     1201 cgttttactc gcgggggaaa ctaatggtta acgatctgac gtataagaaa ttagaaagca
     1261 caaacatcaa ctgtaatatt ctctcgccag aaaataccac atggacaaat gtcacggtga
     1321 ttggcctgga tctttttgcc atttgcggcg tgtgcagttt gctcaagcgt gttgggggag
     1381 tgaaacttca ggttttcact tccttaccca ctgatatgaa tgttctgact gacagtagca
     1441 tcgtgatatg ggtaaaaatg cgtcacgatg gtttgcctga attagccgga catattgtga
     1501 aggtttgtcg tcgataccgt ctgatgcggc aactcgttat tagcgatgct attcctgaag
     1561 gaatgacgaa tgatatcggg cctctttccc gagtatggct tactcatggg agtgcaaaag
     1621 tggatatttt attatcttca ttacggaagg tgttaaatgc tcattgtttg cctggtcctt
     1681 tgtttataaa acgcctggga agagtgcaat ggagggttct tttactccgc gcgaagggga
     1741 ttagcacacg taaaattgca gaaatctgtg gtattggcct caagaccgtt agcgcccatg
     1801 agtccgcaat tcgagaacga ttaggcacca ggaataaaag tgagtacgtc tggttagtac
     1861 gtaatgctca acttatacaa caggcgatac ccgcgttggg aagagatgcg tcaaggtatg
     1921 aaagatgtaa caatatgcga acaaagcaaa aagtataatt gatgcgtcat agataactct
     1981 ctttttcgat gacgaaatcg ctgagcatgc gtggtgaggt tagtaataga tgggtgctat
     2041 gttttgccac gggtttgtag ctgtgaaacc tggaaaaaaa taagaattaa aaatatttaa
     2101 agagagttaa taaggttctg gttgtaattg tcttggtcaa aaaaaagata ttgtcaatgt
     2161 tgttttttaa attttcaggt tttggttaaa tatttgcttg caaatatagg tgattttttt
     2221 attgtag
//
LOCUS       DAEBXN010000281         2225 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.286, whole genome shotgun sequence.
ACCESSION   DAEBXN010000281 DAEBXN010000000
VERSION     DAEBXN010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.286"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            249..1286
                     /gene="curA"
                     /locus_tag="KJL99_004476"
     CDS             249..1286
                     /gene="curA"
                     /locus_tag="KJL99_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HBD4438934.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATE
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHATEVLGFDVCLDHHADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYREDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            1484..2149
                     /gene="mcbR"
                     /locus_tag="KJL99_004477"
     CDS             1484..2149
                     /gene="mcbR"
                     /locus_tag="KJL99_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HBD4438935.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRQSQLDEINRIRYELELMAVALAVE
                     NLSSQDIAELQELLGKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWI
                     RMGPALHYLYEAINPAELRERIENYHLLLAALKAKDKEGCKHCLAEIMQQNIAILYQQ
                     YNR"
BASE COUNT          612 a          471 c          599 g          543 t
ORIGIN      
        1 cgttcgagca ttataacgaa tggcatccgc atagtgcgct gggttatcgc tcgccacggg
       61 aatatctgcg gcagcgggct tgtaatgggt taagtgataa cagatgtctg gaaatatagg
      121 ggcaaatcca acggattcct cgatgaaatt attgcgtgtt gcagagggga tgtgacggct
      181 gcaaacaaaa ccaatcccgt gagcctgcta ctattgatgg attcatgcca aaagcacagg
      241 agagcattat ggggcaacaa aagcagcgta atcgacgttg ggttctggcc tcgcgtccac
      301 atggcgcacc ggttccggag aatttccgtc ttgaagaaga tgatgtcgcc acaccgggtg
      361 aaggacaggt gttactgcgc acagtttatt tgtccctgga cccgtatatg cgtggacgta
      421 tgagcgatga gccatcttat tcaccgccgg ttgatattgg cggcgtgatg gtcggcggta
      481 cggtgagccg tgtcgtggag tcgaatcatc ctgattatca gcctggcgac tgggtgctgg
      541 gctacagtgg atggcaggac tatgacatat ccagtggtga tgacctggtg aaacttggcg
      601 atcatccgca aaatccatcg tggtcgctgg gtgtgctggg gatgccaggc tttaccgctt
      661 atatggggct gctggatatc ggtcagccta aagagggcga aacgttggtg gtagctgcgg
      721 cgacagaacc agtgggggcg acggtggggc aaatcggcaa acttaaaggt tgcagagtgg
      781 tgggggtagc cggtggcgcg gaaaaatgcc gccatgctac cgaggtgtta ggcttcgatg
      841 tttgccttga tcaccacgcg gacgattttg ccgaacaact ggcgaaagcg tgcccaaaag
      901 gtattgatat ctattatgaa aacgtgggcg gtaaggtatt tgatgcagtg ctaccattac
      961 ttaatacatc tgcgcgcatt cccgtttgtg gcttagtgag cagctataac gctacagagc
     1021 taccacccgg tccggatcgt ttacccctgt tgatggcaac ggtgctgaaa aaacgtattc
     1081 gcttgcaagg gtttattatc gctcaggatt atggtcaccg catccatgag tttcagaagg
     1141 agatggggca atgggtgaaa gaggataaaa tccactaccg cgaagatatt actgatggtt
     1201 tagagaatgc gccacagacg tttatcggcc tgctgaaggg taaaaacttc ggcaaagtgg
     1261 tgatccgcgt cgcgggtgat gattaaagtt tcaacggcgg cgtaagccgc cataaaaatc
     1321 caggcaaaaa gcttattttc agctttaatt aactaaccac gtcattgttc tgctgctaat
     1381 aacaacaaag gtgggtggtt tatgattatt cactttaata cactaggtga actccttctg
     1441 ccatgcaggc agggttggac agaaaacacg cgaaggaatt aaaatgcctg gaacgggaaa
     1501 aatgaaacat gtcagtttga ctctgcaggt tgagaacgac ctgaaacatc agcttagtat
     1561 tggcgcatta aaacctggcg cacgcctgat tactaaaaat ctggcggagc aattaggtat
     1621 gagtattaca cctgtgcgtg aagcattatt acgtctggtt tcggtgaatg cgctttctgt
     1681 cgcacctgca caagcattta cagtaccgga agtgaggcaa agtcagttag acgaaatcaa
     1741 tcggatccgc tacgaactgg aattaatggc agttgccctg gctgttgaaa acctctcctc
     1801 gcaagacata gcagaacttc aggaattgct cgggaaatta cagcaggcgc aagaaaaggg
     1861 cgacatggaa caaatcatta atgtgaacag gctatttcgc ttagcgattt atcatcgctc
     1921 aaatatgccc atcctgtgtg agatgattga gcaactgtgg atcaggatgg gacctgcttt
     1981 acattatctt tatgaagcga ttaacccagc ggaattacgg gagcgcatag aaaactatca
     2041 tctattactc gctgcgttaa aagcaaaaga caaagaggga tgcaaacatt gtcttgctga
     2101 aattatgcaa caaaatattg ctattttata ccagcaatac aatcgttaaa ttaaatgccc
     2161 ggcaaatacc gggcatcaag taattactca aatcgccacg caatattcat gccgacacca
     2221 taatt
//
LOCUS       DAEBXN010000282         2218 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.287, whole genome shotgun sequence.
ACCESSION   DAEBXN010000282 DAEBXN010000000
VERSION     DAEBXN010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.287"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(100..525)
                     /gene="arsC"
                     /locus_tag="KJL99_004478"
     CDS             complement(100..525)
                     /gene="arsC"
                     /locus_tag="KJL99_004478"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HBD4438936.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(538..1827)
                     /gene="arsB"
                     /locus_tag="KJL99_004479"
     CDS             complement(538..1827)
                     /gene="arsB"
                     /locus_tag="KJL99_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HBD4438937.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(1881..>2218)
                     /gene="arsR"
                     /locus_tag="KJL99_004480"
     CDS             complement(1881..>2218)
                     /gene="arsR"
                     /locus_tag="KJL99_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HBD4438938.1"
                     /translation="IQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQPKISRH
                     LALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRNLARQNC
                     SGDSKNICS"
BASE COUNT          589 a          610 c          555 g          464 t
ORIGIN      
        1 gaatttgcac ggttttgtag gtcggataag acgcgttagc gtcgcatccg acataaacaa
       61 agcgcacttt accaactatc ggcagtggat atcgccgctt tatttcaggc gcttacccgc
      121 ttcatcaacc actttctcgc catcttcctt ggagaatgcg cctttttgcg catctggcag
      181 aatttccagc accacttctg aagggcggca caggcgagtt cccagcggcg tcaccacaat
      241 cgggcgatta atcagaatcg ggtgctgaag cataaagtcg attaaccgat cgtcagtaaa
      301 tttatcttcc gcaaggccca gctcctcata cggttcgacg tttttacgca gcagcgcgcg
      361 tacggaaatc cccatatcgg caatgagttt gaccagttca tcgcgcgttg gcggagtttc
      421 cagataatgg ataatggtcg gttctgtgcc gctgttgcgg atcatctcca gcgtattacg
      481 cgacgtgccg caggccgggt tgtgataaat ggtaatgttg ctcatatcag tatctcatta
      541 caaagtgaaa gagagacgta gcgccagcgc agccagcgtc acaaacagca caggcagggt
      601 catgataatc cctgtacgga agtaatatcc ccagctgata gtcatattct tctgcgaaag
      661 tacgtgcagc cagagcagcg ttgccaggct accaatcggg gtaattttcg gccccaaatc
      721 gcagccaatc acgttggcat aaaccatcgc ttctttgata acgccagatg ccgtgctgcc
      781 atcaatggac aacgcgccaa ccagtaccgt cggcatattg ttcataatag aagagaggaa
      841 ggcggtgagg aatccggtgc cgagcgtcgc ggcccacagg ccgttatccg ccagcacgtt
      901 gagtacgcca gaaagatatt ccgttaatcc ggcattgcgc aggccataaa ccaccagata
      961 catgccgagc gagaagatga caatctgcca gggggcaccg cgcaggactt tacccgtatt
     1021 aatcgcatga ccgcgtttag cgacgacaaa taatatcagt gcgcccacag ctgcaatggc
     1081 gctcaccgga atgccgagcg gttccaggac gaaaaatccc accagcagaa gcagtaaaac
     1141 aacccagcca gttttgaacg tagcaggatc tttgatcgct tctgcgggag atttcagcag
     1201 cgccatatcg tagttctgcg gaatatcttt gcgaaaatag agatgtagca tcaccagcgt
     1261 ggcaacaatc gcggcgatat ccaccggcac catcaccgag gcgtattcgc gaaagccgag
     1321 gccaaagaaa tcagcggaaa cgatattcac taggttggag acaataagcg gcaggctggc
     1381 ggtatcggca atgaatccgg ccgccatcac gaacgccagc gtcgtgcctt tactgaatcc
     1441 taaagccagc agcatggcga tgacaatcgg cgtcaaaata agcgccgcgc catcattggc
     1501 aaacagggcc gcaacggcgg caccgagcag gacaatccag gtaaacagca agcgaccgcg
     1561 tccattgccc cagcgtgaaa cgtgcagcgc tgcccattca aaaaagccgg actcatccag
     1621 cagcaggctg atgataatga cggcaataaa cgccgccgtc gcgttccaga cgatattcca
     1681 caccaccgga atatcacccg gatgaaccac gcccgtaacc aacgccagta ccgcgccgag
     1741 cgttgcactc cagccgatgc ctaaaccttt cggctgccag ataaccaata cgatggtcag
     1801 gacaaagata gcgcctgcca gtaacataat gcctcccgga taaaacacat gtgcaaatcc
     1861 atatgtgttt agctaaattt ttaactgcaa atgttcttac tgtctccgga acagttttgt
     1921 ctggccaggt tgcggacaat tgcctgaatc ttttcctgtt cacatcgcca ggcttcatca
     1981 ataattttcg ccgcccatgc tggaatatgc ggcgataagc ggtaatgaac ccacttacct
     2041 tgcttgcggt ccagcaatag cccgctttca cgcagcaatg ccagatggcg ggatatcttg
     2101 ggctgcgact gatcgagggc agtgcagaga tcgcagacgc ataactctcc cagttcgcta
     2161 agcagtaaaa cgatgcccag acgggtttca tcagcaagaa ttttgaacaa ttggatgg
//
LOCUS       DAEBXN010000283         2198 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.288, whole genome shotgun sequence.
ACCESSION   DAEBXN010000283 DAEBXN010000000
VERSION     DAEBXN010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.288"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            440..1153
                     /locus_tag="KJL99_004481"
     CDS             440..1153
                     /locus_tag="KJL99_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000082917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438939.1"
                     /translation="MSQQVVIFNNEFSMSSYEFLIEVVNPARAEAGEAPVRNDQFLAR
                     VRDELDDCKTFATERITPRGGGRQMESITLTGDQLLLVGMRESKAVRRKVLDYIRRIE
                     KDKQLLEDQKKRAAIQSANRRGVTWGDYCKTYGLPAQKLMTALLQHRGLFRKNPISNE
                     WSVNPKYSDCFRIIKPSDQKFSAGGYNFRFNAKGLEVFGKPTTVDKLRGILIAFTGTD
                     QQKQEHLLKQAQSGKLEGL"
     gene            1470..1961
                     /locus_tag="KJL99_004482"
     CDS             1470..1961
                     /locus_tag="KJL99_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438940.1"
                     /translation="MLFNNQDWKLSVTDINLYENTVSLDGQSYPLSLAIKTLIPGYLS
                     GLPSTSREAMELLEALAEAGVTIGNFFSNDLMTAYQRRQMNKRIEAERITKELASQKE
                     RTREMFMTEAEWQKELQRREQVKTERRTYGENLRSATHSAGRSKASIMADLDSGANWM
                     DSI"
     gene            2026..>2198
                     /locus_tag="KJL99_004483"
     CDS             2026..>2198
                     /locus_tag="KJL99_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438941.1"
                     /translation="MTLTKDALQFGIEPVRITDTDNLQINEGLPTNADPQVYAFQLAK
                     LVKTMLNSVLKDA"
BASE COUNT          566 a          584 c          534 g          514 t
ORIGIN      
        1 catacttcgg gtctaactgg aggtcagatt atggccttat caggtagcaa atcttatacc
       61 ctcactatag ggaatggacc cttagctatc actataggga ccaacattcc gcctctggtc
      121 ttgatgcgtc aagttgtgcg gaagctctcc cgacattaag tctttctcca taccaaggct
      181 agcaagacag cctctgtcag actcccagcc cgttagcgtt ctcgtgtctt cattttgact
      241 tgggtctatc gccgctcacc atctggacgt cggacatgct gtcttgcacc tttatcacta
      301 ctggtcatcg cagaccacat cacaattacc tcaagtcatt ctgtttgcgt cctgttgcct
      361 gctggtctcc ctttacgtgg gggcttgagt aggtcagtag ggtgcgactt cagggtgcaa
      421 acaaaaggag tacacgtaaa tgtctcaaca agtagtcatc tttaacaatg aattctctat
      481 gtcttcctat gaatttctaa tcgaggttgt caaccctgcc cgtgcagagg ctggcgaggc
      541 tccggtccgt aatgaccaat tcttagcccg tgtccgcgat gagctggacg attgcaaaac
      601 ttttgcaacc gaacggataa cacctagagg aggtggtcgc cagatggaat caatcacgct
      661 gaccggagac caattgttac tggtcggcat gcgcgagtct aaagctgtgc gtcgcaaagt
      721 ccttgactac atccgacgaa ttgagaagga caagcagtta ttggaagacc agaagaagcg
      781 agcagccatt cagtctgcta accgtcgagg cgtgacttgg ggggactact gcaaaaccta
      841 tggtctgccc gctcagaagc taatgaccgc acttcttcag catcgtggtc tctttcgtaa
      901 gaacccaatc agtaacgagt ggtctgtaaa tccaaagtat tccgactgct tccgtatcat
      961 taagccgtca gaccagaagt tctcagcagg tgggtataac ttccggttca acgcaaaggg
     1021 actggaggtc ttcggtaagc cgacgacagt cgataaactg cgaggcatcc ttattgcctt
     1081 cactggcacc gaccagcaga agcaagagca cctcctgaag caggcccaat ccggcaagct
     1141 ggagggactc taagatgatt gacccgcgca cacctgaggg ccgcatgact ctccgttacc
     1201 gtggctaccg gactgaagtc atcctgaagg agcttggtct agacccggaa gatgagaccc
     1261 gccagcacca atcacgagac gagctaatag cccaactggt cgctatgaaa ctgtcgtcgg
     1321 ctagcaagct agcgccagca catcgttagt agctcaccta cttaatacct aattttaaac
     1381 cttttgagat gaccgatgcg gggagacgcc gcaggactaa acacgcctgt agcagaccga
     1441 tagtctcaga ctcaagagga gtaattacta tgttattcaa taaccaagac tggaaactgt
     1501 cagtcaccga catcaatctc tacgagaata ctgtgagtct ggacggtcag tcctatccgt
     1561 tgtcgctcgc tatcaagacc ctgattccgg gctacttgtc cggcctgccg tctaccagcc
     1621 gcgaggcaat ggagttactg gaagctctgg ctgaagctgg cgtgaccatt ggtaacttct
     1681 tcagtaatga cctgatgact gcctaccaac gccgccagat gaataaacgt atcgaggctg
     1741 agcgtatcac taaagagctg gcgtctcaga aagagcgtac ccgcgaaatg ttcatgaccg
     1801 aggccgaatg gcagaaagag ctacagcgcc gcgaacaggt caaaactgaa cgccggacct
     1861 atggtgagaa cctccgaagc gctacccatt ctgctggtcg ttccaaggct tcaattatgg
     1921 ctgacctcga tagtggcgct aactggatgg attccatcta agaacacacg gtccctgact
     1981 taccgttagg gacttcaaat atctgactca aggagattaa ttatcatgac tctgactaaa
     2041 gacgctttac agtttggcat tgagcctgtt cgtatcaccg acaccgacaa ccttcagatt
     2101 aacgaaggtc tgccgaccaa cgcagacccg caggtctatg ccttccagtt agctaagctg
     2161 gtgaagacca tgctcaattc agtgcttaaa gacgctca
//
LOCUS       DAEBXN010000284         2187 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.289, whole genome shotgun sequence.
ACCESSION   DAEBXN010000284 DAEBXN010000000
VERSION     DAEBXN010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.289"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(220..384)
                     /locus_tag="KJL99_004484"
     CDS             complement(220..384)
                     /locus_tag="KJL99_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438942.1"
                     /translation="MTLTTTALNGSSSRRFEGCVWTPPLISHTALHPTSRHWMHSWHT
                     YAGNAEIPGL"
     gene            1286..1570
                     /locus_tag="KJL99_004485"
     CDS             1286..1570
                     /locus_tag="KJL99_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HBD4438943.1"
                     /translation="MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMA
                     CKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDPAIEKLLARKPQWDV"
     gene            1558..2043
                     /locus_tag="KJL99_004486"
     CDS             1558..2043
                     /locus_tag="KJL99_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBD4438944.1"
                     /translation="MGCVTAPEPLSSFHQVAEFVSSEAVLDDWLKQKELKNQAIGATR
                     TFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNMPDPIPVIILARLAVDVSFRGKG
                     LGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
                     Q"
BASE COUNT          565 a          462 c          620 g          540 t
ORIGIN      
        1 tcaaaatgat ggacattatt tttgtggaac cggaggtaac agaccagacg gtttaaatga
       61 gccggttact ttaaacgggt gatggacagc acctcaaaag ttcagatgga ttatatttgt
      121 ctgcaatacc ccgggtattt tcgatttgcg aaatggatgg aacgacttgc tcaggggata
      181 gctgatggag ttactgaagt accgaaggac cactaaaaat cacaggccgg gaatttcggc
      241 gtttccggcg taggtgtgcc acgaatgcat ccagtggcgt gatgttggat gcaatgctgt
      301 atgagagatg aggggcggcg tccagacgca gccttcaaac cgccttgagc tgctgccgtt
      361 aagagcggtg gtggtgagcg tcaaggttaa aaggcgcaga acatgtgtca ggtaggtgtt
      421 gcggagatga acgaaagtga actgtcgatg aagtgtcgaa aggaacctct aggcgatgtc
      481 gaaaatgtgc tatctacgca gcgcatgacg agtccagcag atatctggta ctggctgggc
      541 ggcatccggc atataggcag catgaacgca acacaggctc tcgtacggaa cgtgggaacc
      601 tgtcgtctcg atgctaaggg agcggcgcgg agcagaggca ctgcaagcgc ctgagtaccg
      661 atgcgagaca caggggcaga tggcttcgta gtagtgagga agtgcctgta atgggcatgg
      721 agcgaagggg gtcagtcgtt ggggctggtt tatcggtcaa ccggatagcc gggaggagcc
      781 catgagtcag ccaaagccgt tcgaggtctc taagtatgcg gtttggaaag cataccagcg
      841 ggtcaaagct aatcgaggtg cggcaggtgt cgatgggcag tcagtcgagg cgtttgaggt
      901 taccaatcga agctataccc ggtaatggcg caggttgacc tgcacttggc caagtggatt
      961 gcccgcaaac acaagcgagc gcgaggtagt ctggtgcgtg cattcgagtg gttactgcga
     1021 attcgtcacg actgcccgac gctgtttgca cactggtgct tagcttacga tacttgaatg
     1081 gttcaacgac aagagccgta tgacgcgaga gtgtcacgta cggctccgtg ggagcgtagg
     1141 ggggcggtgc ccctgcgcca cccgatatcc cctttgagtg cggttacgtc cacagaggta
     1201 aatgccgctg acgcggcatc tgtaatctgt tactttgtgt atatcatttg ctatatattg
     1261 tcaatatgct attgttgtaa agcaaatgat atacggaggc tttatgaaat caggtgttca
     1321 gctcaatctc agagcaagag aatcacagcg gatactcatt gatgctgctg cagaaattct
     1381 tcataagtcg cgtacagatt tcattctgga aatggcctgt aaagctgctg aagatgttat
     1441 tctcgaccgg cgggtgttta attttaatga caggcaatat gaagagttca ttgaaatgct
     1501 tgatgctccg gttgcagatg atcctgccat cgaaaaattg ctggcaagga aacctcaatg
     1561 ggatgtgtaa ctgcaccaga acctttatcc tcatttcatc aggtcgctga atttgtcagc
     1621 agtgaagctg tacttgatga ctggttaaag cagaaggagc taaaaaatca ggcaatcgga
     1681 gccacccgga cttttgttgt atgcagaaaa ggtacgcaac aaatagtcgg tttttactca
     1741 ctggccactg gtagtgtcaa tcatacagaa gctacaggta accttcggcg caatatgcca
     1801 gaccccattc ctgtaattat acttgcacgc ctggctgtag atgtgtcatt tcgaggaaaa
     1861 gggcttggcg cggatttact tcatgatgca gttcgtcgtt gttacagggt tgccgaaaat
     1921 atcggtgtgc gtgcaattat ggttcatgcg cttacggaaa acgcaaaaca gttttatatt
     1981 catcatggtt ttaagccatc aaaaactcag gtgcaaacat tgtttcttaa actccctcaa
     2041 tagggagtaa tgccgatcag ttaaggatca gttgaccgat ccagtggctg tgtaagaatc
     2101 cggaaacgct cactcgtttc cggatttttc tgtgcacagt aaaagaccac ctcctgagga
     2161 ggtggctttt cagaatgccc cctataa
//
LOCUS       DAEBXN010000285         2183 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.290, whole genome shotgun sequence.
ACCESSION   DAEBXN010000285 DAEBXN010000000
VERSION     DAEBXN010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.290"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            1..694
                     /locus_tag="KJL99_004487"
                     /pseudo
     CDS             1..694
                     /locus_tag="KJL99_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748481.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            696..1301
                     /locus_tag="KJL99_004488"
     CDS             696..1301
                     /locus_tag="KJL99_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HBD4438945.1"
                     /translation="MQNVAATVLAQYAASPRLNALINSFNAALSPDSFISDFYGLIWN
                     IDTAEKYGLDVWGKIVGVSRWLTVKDDFNYLGFSESRMDTPVMDDPCPFNQAPFYNGK
                     SDTRTVDLSDAVYRRLILMKAMSNITDCSVPDINLMLRFMFGKKRRAYVLNNGGLRMS
                     YVFESALSLAELAIIQSSGALPSPPGVYVSVVLKESRNEGQ"
     gene            1288..>2183
                     /locus_tag="KJL99_004489"
     CDS             1288..>2183
                     /locus_tag="KJL99_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBD4438946.1"
                     /translation="MKASDKPRQLAVPFASTGDKNRIPDKATQQTRESGNAAYDSGFP
                     PVTMTAVSAGGIPPHGKDFNGLMYDITAAIRFAQAGGLYTYNAGFAGAIGGYAKGAIL
                     AGVATTAVWLNTTDDNLTDPEGSDSAGWVNLLEDPKRIFLRQKNNLSDLQNKGTARDN
                     LQVYSKEQSDQRYVHREGDKITGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSE
                     GQGENGDIGPLRPFTINLRTGEISMSHKVSVGGGSQVNGALGIGVQNALGGNSIVLGD
                     NDTGFKQNGDGILDVYANSQRV"
BASE COUNT          531 a          507 c          609 g          536 t
ORIGIN      
        1 cccgtgccgg tgtgccgggc agcgatgagg aaacccgtgc ggactttgaa aaccgccgac
       61 gtaattcagt tgcccgtaat gcccggaata ttctggaagc catccggggt gaaatactct
      121 ccacggtaga aaacgtggtg gatgtttacg tcacccataa tccgaaaaaa acagaacaaa
      181 aagccggagt cagtatccat taacacccgg ttcgctttat gttggcgtgt acggcggcag
      241 cccggcagat atcgcggcgg ccatctggcg taaggctccg ccgggtattg atatgaatgg
      301 caacacaacg ttcactgttg cagataagga gtacgatccg ccgtatcctg aatacgtgat
      361 cagctggcag acactcaaac ccgtcagtct gcatgtcagt gtgacgctga aaaaaagtga
      421 ctacctgcct tcagatatta cccgacaggt acagcaatcc gtgctggacg catttaatgg
      481 tacagatggt ggtctgcggg caagagttgc ctctgttgtc tctgccgggc gctactatgc
      541 cggcatttac aaaaccgatc cggaacatat tgatattctg ggccttactg tgagccgtga
      601 tggttcgtca tggacaacgg ctgtcacttt cgggatagat gagattccgg ttctggatgt
      661 gtcggatatc agtgtgaaac tccaggaggc ataacgtgca gaatgtggct gccactgtgc
      721 ttgcgcagta tgctgccagc ccccgactca atgccctcat taacagcttt aacgcagcgc
      781 tttcccccga cagttttatc agtgattttt atggtcttat ctggaacatc gataccgcag
      841 aaaagtatgg tcttgatgtc tggggaaaga ttgtgggtgt cagtcgctgg ctgacggtaa
      901 aggacgattt taattacctg ggcttcagcg agtccaggat ggacaccccg gtaatggatg
      961 atccctgtcc gtttaatcag gcaccgtttt acaacggaaa atcggatacc cggactgttg
     1021 acctgtctga tgctgtatac cggcggctga tactgatgaa agccatgtcg aacattactg
     1081 actgttccgt tccggatatt aacctgatgc tgagatttat gttcggaaaa aaacgccggg
     1141 cttatgttct gaataatggc gggctgagga tgagttacgt ctttgagtcc gcgctctcgt
     1201 tggcagaact ggcgattatc cagtcgtcgg gtgcactgcc atccccgccg ggtgtttatg
     1261 tttcagtagt tttaaaggag tcccgtaatg aaggccagtg ataaaccccg ccagctggcg
     1321 gtcccctttg cgagtaccgg agataaaaac cgtatcccgg acaaggcgac acagcagacc
     1381 agagagagcg gtaatgctgc gtatgattca ggttttcctc cggtgaccat gacagcggtc
     1441 tcagcgggag gtataccgcc acacggcaag gattttaacg gtctgatgta cgatattacc
     1501 gcagcaatac ggttcgccca ggctggcggt ttgtacacgt ataatgccgg ttttgcgggg
     1561 gccattggtg gatatgcaaa aggagccatt ctcgccggag tcgcaacaac agcggtctgg
     1621 ctgaatacca cggacgataa cctgaccgat cctgaaggct ccgacagtgc gggctgggta
     1681 aatcttcttg aggatccgaa aaggatattc ctgcggcaga agaacaatct gtcagacctt
     1741 cagaataaag ggacggcacg ggataatctt caggtttaca gtaaagagca gtcagatcaa
     1801 cgctatgttc atcgggaagg cgataaaata accggagagc tgaaaatccg tggtgttaat
     1861 gcgctgagga ttttcaacga agcttttggc ctgatttttc gtcgttcgga agagtgcctg
     1921 caccttattc ccaccagtga aggtcagggg gaaaatggcg atattggtcc cctgcgcccg
     1981 ttcaccatta atttgcggac gggtgaaata tccatgtcgc ataaagtgtc tgttggcggt
     2041 ggttcgcagg tcaatggtgc gctgggtatc ggcgttcaga acgccctggg ggggaattca
     2101 attgttcttg gtgataatga caccggattt aaacagaatg gagacggtat tctggatgtt
     2161 tatgctaata gtcagcgtgt att
//
LOCUS       DAEBXN010000286         2169 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.291, whole genome shotgun sequence.
ACCESSION   DAEBXN010000286 DAEBXN010000000
VERSION     DAEBXN010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.291"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            6..224
                     /locus_tag="KJL99_004490"
     CDS             6..224
                     /locus_tag="KJL99_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HBD4438947.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPQIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            226..591
                     /locus_tag="KJL99_004491"
     CDS             226..591
                     /locus_tag="KJL99_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBD4438948.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPECHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEIYQEGATA"
     gene            588..1253
                     /locus_tag="KJL99_004492"
     CDS             588..1253
                     /locus_tag="KJL99_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438949.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFYKAEVERWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            1253..1618
                     /locus_tag="KJL99_004493"
     CDS             1253..1618
                     /locus_tag="KJL99_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HBD4438950.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVTLAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
     gene            2073..>2169
                     /gene="hokD"
                     /locus_tag="KJL99_004494"
     CDS             2073..>2169
                     /gene="hokD"
                     /locus_tag="KJL99_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HBD4438951.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEV"
BASE COUNT          595 a          450 c          579 g          545 t
ORIGIN      
        1 aatgaatggt tacattattt agaaaaaaat atccgcgaaa gagtagaaca acagaattcc
       61 tgtttctcat tctgtttatc gtgttgatga caccgatatc cccgcaaatt tttgtctgga
      121 taatcggaaa aataattgag ccagttattg aattgtataa cgacgtggta tgggcgtcat
      181 tcaacacact gcacaataaa attaatccgt ataaggaaag ctgatatggc actgacgaaa
      241 aaacaacgtg cagaactgcg catgaagttc ggtggtcgct gcgcttattg cggctgcgaa
      301 cttggcgaaa agtggcatgc agaccatgta aaaccggtca ttcgttttga tggaaatatg
      361 cttcaccagg aacgtgacga tatatccaac atggtaccag aatgccaccc atgcaatctg
      421 cacaagcatt gcagtagcct ggaagattat cgacgaatta tcagtgatgg tcgtcgtgaa
      481 ttccttgcgt ccgggaaagg caaagcgctg gttcgtatgg gattggttga aatgaaatct
      541 gacccggttg tgttctggtt tgaaatatat caagaagggg ctacggcatg actactatta
      601 ccaaagagcg actgcaatgg ctggctaaca tttctggccg cgatgatatt gacgatatag
      661 acggcggtga aattcgtgaa ctggcgctta tcgctctggc atcactggaa gcagagcctg
      721 tagcggaatg cattgttgaa gatgggggca tgtgtattga tgggttcggt gagtatgtgg
      781 gtcactcgct gcctgatgga atgcatgagc tttatgctgc cccgcgaatg cgacaacctg
      841 atggatggaa agccgtagct gtagcgtgga aggtgacgtt tactcaagtt gaccaggaat
      901 ctaatacgtt cactgctata tatttttaca aagcggaagt tgagcgttgg gtacgactgc
      961 ataaagcatg tgattttcgg gcagatataa caccgcttta cgcagcgccg gcagtgccgg
     1021 ttgcagtaaa cgacgacatg gcttacgcat tccatcacgc actgtcagat tcatcgcttg
     1081 gctctgatga aatcgaagaa attaaaaccg gtttgcgtgc tgcctttgcc aacgtcacta
     1141 tccaaccaga gccgatagtg ccggatgaaa tcgggccaaa cgatagtaat acgtttgatt
     1201 atgttgatgg ctggaacgcc tgccgcgctg ccatgcttaa gggagataaa taatgattaa
     1261 tcgaaccaaa ctggagcaca ttctcgaata tgccaggcag cagaaatgta ttgggcaact
     1321 ttgtaaaatt ccaccaggag atatggttga aatcgtggaa atggccatgc gtaaggctgg
     1381 caactctccg gtaactctgg ctggctggat aagctgtagt gatgcagttc ctgctgaata
     1441 ttgcgatgtg attcttctcg atgatctcgg gaatgtattc cccggttcct gggataaggt
     1501 tttttgcccc actcgtggcg ggaataagat ggcttttgtg gacaaagacg gcgttgaagt
     1561 agagagctca actcactgga tgccgctacc ggaaccaccg caggaggtga atcaatgacc
     1621 tggcctgagg cattcaccac ggtaggaatt gcgatggcgg tggcgctggt ggtgtattcg
     1681 atttgccgtt ggggttaaca aacaaaaacc ccggattgat ggtccggggt ttttgaagga
     1741 aacaaaacag aaacagcaat tgccgttacc tgttgttacc atggcaagta aacgtatctc
     1801 aggcgagcgc attgcgccgt tctgacgcag ataaattagc ctggatagaa ggtgctggca
     1861 ataaaaaata gcgttttctt atcggtatcg gtaagattgc tgcgggtgct tgaggctgtc
     1921 tgcctcgggc atgccactgt aaggcagaca gagaaaagcc ccagttaaca ttacgcgtcc
     1981 tgcaagacgc ttaacattaa tctgaggccc aatctatgtc tcacaaatgt aggttagcct
     2041 cttacgtgcc gaaaggcaag gagaagcagg ctatgaagca gcaaaaggcg atgttaatcg
     2101 ccctgatcgt catctgttta accgtcatag tgacggcact ggtaacgagg aaagacctct
     2161 gcgaggtac
//
LOCUS       DAEBXN010000287         2162 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.292, whole genome shotgun sequence.
ACCESSION   DAEBXN010000287 DAEBXN010000000
VERSION     DAEBXN010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.292"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            80..259
                     /locus_tag="KJL99_004495"
     CDS             80..259
                     /locus_tag="KJL99_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309392.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438952.1"
                     /translation="MDLSPEDAKFLRELQHRMLREAIIEQQTGMSAIDPLRYKRASSP
                     LVDDNSNADKTRPPH"
     gene            311..505
                     /locus_tag="KJL99_004496"
     CDS             311..505
                     /locus_tag="KJL99_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438953.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            complement(1304..1640)
                     /locus_tag="KJL99_004497"
                     /pseudo
     CDS             complement(1304..1640)
                     /locus_tag="KJL99_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          688 a          444 c          450 g          580 t
ORIGIN      
        1 atactggttg tctggagatt cagggggcca gtctaggtag cgtaaggcac tacagcgcat
       61 tgctttacga gtaataacaa tggatttatc accagaagac gcaaagtttt tacgagaact
      121 gcaacaccgt atgctcagag aagccattat cgaacagcaa acgggcatga gcgccataga
      181 cccattgcga tacaaacggg cctcatctcc tttagttgac gacaattcca atgcggataa
      241 aacacgtcca ccacactgat tacatttttc attagcacag tggtgtgatg cagcatacga
      301 ggaaaaccgg atgaatgaga acaaaataaa aaggctggag caactgttac aggcacgcca
      361 acgggagttt gccactaaat ggggacgtgc cgcgctgcca catgaacgcc taacctattt
      421 tccacttgaa gacctcatta aaaataatgg caaaaaaatc aggtctgata agaaacactt
      481 cttagaatgc aatgaagaca aataacaaca atctgcatat tgccaccaac cacagatgtg
      541 gcatcgttat cagcgcactt gattttcttt atattcactg acgagcttgc cggggcacct
      601 ttgtttgccg gtcattctgg tatcatgcag atatttacgg cgtacaagcc cactagcatg
      661 acattgtaat aaggcctgag cctaacgctt taatgtgtgt catacactca gtggcgtaat
      721 cgttgacaat atggtgcatg taatcgtaag cgtccagcga aatccatctg gtcataatct
      781 gaagcatccg acaaagtggt gtccaccaaa taagtgatct gcgcccaata tcctgagccg
      841 gaactttgac aaaggccaac gaaaaatggg agcggatgtt acagagttct cggtacaggg
      901 gaaaagtggt acctgtcgcc agttctccga tctgtttaac cgggaaatta tctcctacag
      961 cttgtcagaa aggtcggtga tggagcatcg ttaatacgat gctatacgat gcattcacag
     1021 tgcccggcca gaggatgccc cgtcgctgca tatggatcag ggttggcaat atcgaatcgc
     1081 aggctatagg caaagttaaa gccccatgga gtagcacaaa atatgccgca caaaggaaac
     1141 ggtctgaata acgcagtgat gaagaacttc ttcagcacac tgctaaaacg catagtgatc
     1201 gagcgctgat tctggcgaac aactgaacta atacatcaga atctgcatta tgttaaataa
     1261 atatgaaaag atggtttaaa taccccgtta cttttgactt acactatacg gtatcgcatc
     1321 gtttaatatt cgcaccggcc agatttttat ttctattagt tgtcacaata ctgaatgcgt
     1381 acgaccacag tattctggct cctgtgtggt tatgctttaa ttctgcgttc cgggcagata
     1441 agcagttgct tgcaggaatc cttcttgtgt taatgtcagt tccccttttt accagtgctg
     1501 atttccacat tccgtccaac agagcttata gcctttccct ggattatagc attgtccggc
     1561 tgaagttctt tttgaataat aatagaagca ctgctggcag atccagtccg tttttcataa
     1621 cccactgtac tgataaccat aatctaatca gtagaaattg agtcgaaaat aagcactact
     1681 ctatacagga taattagagg tcagtttgat tattcacaat tcatcatcag cattttctat
     1741 ttctgacgaa atcaacatga aaataaccat atatgataat tattataata acggctttaa
     1801 ttggaataca tatattacaa cgtattatat ataattggta ttctgggaac tatattctca
     1861 aaatacagta gaaacgaggt atgtttctgg tggaaaggac agtgggatta aaaagtaaga
     1921 attgataaaa aaaacgccag caacacatag tgccggcgga gggaataccc catggagaaa
     1981 atgtgatgcc tagagcatca tgaatatata attaaaaata gttagcgttg tcaatacttt
     2041 cacaaaaata attttcgtag tatagaaaga tatttttatg catgacctac ctgaatttgc
     2101 tccgggtaga ggttataaat aaaaattgaa tcacgacaaa cacaatattc atagtatggc
     2161 ga
//
LOCUS       DAEBXN010000288         2145 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.293, whole genome shotgun sequence.
ACCESSION   DAEBXN010000288 DAEBXN010000000
VERSION     DAEBXN010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.293"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            1..955
                     /gene="espL1"
                     /locus_tag="KJL99_004498"
                     /pseudo
     CDS             1..955
                     /gene="espL1"
                     /locus_tag="KJL99_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            1127..1234
                     /locus_tag="KJL99_004499"
     CDS             1127..1234
                     /locus_tag="KJL99_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438954.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1287..2045)
                     /locus_tag="KJL99_004500"
     CDS             complement(1287..2045)
                     /locus_tag="KJL99_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HBD4438955.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
BASE COUNT          646 a          478 c          447 g          574 t
ORIGIN      
        1 gctcagacct gggctgatag gtatgttcgc aactgtgcaa ttgctattct gcccctatta
       61 cctgtgggag ttctaggggc tattttcgcg ggtattgcat cacgaatgcc atttgcccct
      121 atacatccat cggcaatgtt gttaataatg gctacaggcc agactcaaca gcttattaca
      181 ttattcaaac agttgcccat actccctgaa aaagaaatca ttgaaataat aactgcgcag
      241 aatagcgttg gtacacctgc tttatttttg gctatgatga acggacatac tgacaacgtg
      301 aaaatattta tgcaagaaat tcagtcactg gtagataatc acatcattca tgaagataat
      361 ctggttaaat tactgcaaac taaaagtgct aacgaaacac ctggacttta tatctccatg
      421 ttgtatggat tcgatgaaat aatcgatatc tttctgaata cattaaccac tccaatagca
      481 ctaagagctt ttaaacaaaa aactggtgat gagtatttta gccataaaaa tacatgatgg
      541 tgagccagga ttatacgccg caatggaaaa taatcaccct ttgtgtgtca cacggttcct
      601 ctctaaaatt aatggcatcg cctttaaata caagttgagc aaagctaaca tcatggattt
      661 attaaaagga gctacagcac agggaacccc tgcattatac atcgccatga gcaagggtaa
      721 tgaagacgtc gtgttatctt atatatcgac gctgggtgct tttgcaaaaa aacattcttt
      781 tagtcaacat cagttattta cactattggc cgctaaaaat catgacaaca tgtcagctgt
      841 tcatatagcc attcatcata agcattataa aactgtagaa acatattatg ctgctataaa
      901 tgtcatcagc caaagcctga gttttagtgc tgatgaaata aagacgtatt tataaccagc
      961 tatttacgat atatatcgat acataatttt atttttttca taaaacgcag aaacagtgac
     1021 catgtgcaca acgccacccg acggctgtaa taacaaaaaa tcccttgatg cctgtccctt
     1081 ttgttacact ccgttatcac gcacaagaga tatgcaggac actggtatgc cgactaaacg
     1141 ctttgataaa aaacactgga agatggtggt ggtgctactg gcaatctgtg gcgctatgtt
     1201 gttgctacgt tgggcagcaa tgatttgggg ctgagcagtg acaaacgaag acagccagac
     1261 cgcataacac ggtccggcag ataattttac atgctgtagc ccagcgataa ggttgtccga
     1321 cggtcggtat gttccggtgc agactctgga ggttcagagt tccatgtgac gttgtaagcc
     1381 accttcagtc caaaatgttc attaatagca acatttaatg cgctttcgga gttcagcgtt
     1441 gtgtcttccg cgccaaagac cgaaacaccc tgcgtaaatt tagcgttgtc agtcaactgc
     1501 catgcataag ccccggaggc atagcccagc ggctgggttt cactggcatt gtcggtatat
     1561 ttgtcgtaac gcacacctgg accgaattca aagcggaaac tgtgtaccgg gccattcaaa
     1621 aactgacgac cataacccgc ggtcaacaca tcgcgctctc gatagccgtt ataacggtct
     1681 gtcagccagc ttgcctgacc aaataaatag tcataatcag ttaaattaaa gcggctacgc
     1741 ccgcccgccg catatttttc tgaagaacgc tcatcattag aagaagtatt actggcgttc
     1801 ccccacagcg accaggcagt ggtttgtcca taccaggtca tggtggtgtc agccgtaagt
     1861 gaggagcttt tcgtattgcc tgattgtgca agatatcctg cgttcagatt accttcgaaa
     1921 ggttttttag cgctggcagg atcgtccatg acagtaaaaa cggaatcatc ggcagctgca
     1981 ttcagtgacg caaacatgcc ccccgccaac ataacgatgg caggaactgt cttcaaaagc
     2041 ttcatttatc aagagtccgt acaacaaaaa aagagaccat cgcggtcccg gaaactttct
     2101 taaggatcaa agattagcgt ccctggaaag gtaacgaatt ataaa
//
LOCUS       DAEBXN010000289         2136 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.294, whole genome shotgun sequence.
ACCESSION   DAEBXN010000289 DAEBXN010000000
VERSION     DAEBXN010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.294"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(<1..181)
                     /locus_tag="KJL99_004501"
     CDS             complement(<1..181)
                     /locus_tag="KJL99_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438956.1"
                     /translation="MRKLKMMLFGASLIMVVGCSSKENALCHPQPKPPAPPAWAMMPP
                     SNSLQLLDETFSVSGT"
     gene            complement(434..931)
                     /locus_tag="KJL99_004502"
     CDS             complement(434..931)
                     /locus_tag="KJL99_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HBD4438957.1"
                     /translation="MSAKIKYGLSAAVLALIAAGASAPQILDQFLNEKEGNHTTAYRD
                     GSGIWTICRGATMVDGKPVIPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDVGRDCRIRSNNCYGQ
                     VIRRD"
     gene            complement(997..1626)
                     /locus_tag="KJL99_004503"
     CDS             complement(997..1626)
                     /locus_tag="KJL99_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438958.1"
                     /translation="MKHEEMNQRFNHLENEITELNKKLSALVSSEDENKRRDEHYAAI
                     YDYCHKVAHETFMKFLQEKFLPAALSEKEAAYLRPEYVITVNSAGEEEHKSDFIASAP
                     DKDQEPHRPFRVSCEEGEFVVYENGKPVRASHHHCLKIINLAIRCLKDENTRVMKRIG
                     RCMGYLQVAAEIEALASGADMDAAVREALLRDFNTPPLRKSLMTGSSRG"
     gene            complement(1630..1845)
                     /locus_tag="KJL99_004504"
     CDS             complement(1630..1845)
                     /locus_tag="KJL99_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HBD4438959.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVRPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
BASE COUNT          547 a          569 c          470 g          550 t
ORIGIN      
        1 ccgtcccgga taccgagaac gtttcgtcca gcaactggag agagttcgac ggtggcatca
       61 ttgcccatgc cggaggcgct ggaggtttcg gctgaggatg gcacagggca ttttcctttg
      121 acgagcaccc gaccaccatt atcaagcttg cgccgaagag catcattttc agctttcgca
      181 tcagctaact ccttcgtgta tttagcatcg agtgcatcag catcacgctg gcgctgctgc
      241 atgtcagtaa tggttgcgtt cgccagcttc agttctccgg cgtttttgtc gcgctgctct
      301 ttgtaggtaa tggcgttatc acggtaatga ttaacagccc atgacaggca gacgatgatg
      361 cagataacca gagcggagat aatcgcggtg actctgctca ttgatctatc ccccaacagg
      421 ctaatgcgct ttcctagtca cgacgaataa cctgtccata gcagttattt gaacgtatgc
      481 ggcaatcgcg cccaacatct tttatccacc agcgaatcgc ctcgcatgcg cccttacgat
      541 caccggcatt cagccgctta taaaacgtcg acgggaaaca cttaccgggg ccaatgttat
      601 aggggcaaaa tgacgctata cccgctttct ggggttcggt cagaggtact ttaatattgc
      661 gctccaccca tgccagcgcc ttatcacgtt caatagcgtt aacctggtca catttttcct
      721 tcgacagctt cattcccggt atgacaggct taccatccac cattgtggca ccacgacaga
      781 tggtccatat gccagaacca tcacggtatg ccgttgtgtg attaccctct ttttcgttca
      841 gaaactggtc aagtatctgg ggagcagacg cgcctgcggc aatcagcgcc agaacagcag
      901 ccgacaggcc gtatttgatt tttgcgctca tggatattta tcaggattta tcggcaacaa
      961 ataacgagcc aacttatata cgccttttaa gataagtcag ccccggctgg atccagtcat
     1021 caggctcttt cttaaagggg gagtattgaa atcacgaaga agagcctccc gcaccgcagc
     1081 atccatgtct gcaccactgg caagtgcttc aatctctgcc gccacctgca aataccccat
     1141 gcaacggcca atgcgtttca taactctggt gttttcgtct ttcaggcatc tgatagcgag
     1201 attaataatt ttcaggcagt gatgatgcga tgcccggaca ggttttccgt tctcataaac
     1261 gacgaactca ccctcttcac aggaaaccct gaaaggccga tggggttcct ggtctttatc
     1321 cggtgcagac gcaataaaat cacttttatg ctcttcctct ccggcactgt taaccgtaat
     1381 gacgtactca ggccgcaggt aagccgcctc tttttctgac aatgcggccg gtaaaaactt
     1441 ttcctgcaaa aacttcataa aagtctcgtg agcgactttg tggcagtaat cgtaaatcgc
     1501 cgcataatgc tcatcgcggc gtttgttttc atcttcagaa ctcaccaacg ccgacaattt
     1561 tttattaagc tcagtgattt cattttccag gtgattgaat cgctgattca tttcttcatg
     1621 cttcattatt tactctcccc gtgcagcctt acgcttgtct tctcttattt tgaaatacag
     1681 attcgtcaga taagtcagaa gccccagaag cagacttccc agcacaccaa tcgcagccca
     1741 ctgtgatggc ctgacctgat ccaaccactg caaaaaccag tagccggcac tgccggcgga
     1801 ggtgccgtag gcaatgcccg ttgaaatttt gtccatggat ttcatagcct cacctccgca
     1861 aataacggat ggcgtagttt tacactgaga aatgaaaggg atttgaaaag aaaaaaacgc
     1921 aaaagcgggc gaaacgatat atacagtaag gaaagcactc tatccaacaa accacccaca
     1981 gttaatcgga ataaaagcag agtgcttatg aatgatcgca cgcccgaagg ttagtatttc
     2041 tgcacagcaa ttttgcaaaa aaaggcgatc attcataact taaacgtctt tcagtcactc
     2101 cgggatttcc catcatcgca gactgaaaga ctctga
//
LOCUS       DAEBXN010000290         2132 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.295, whole genome shotgun sequence.
ACCESSION   DAEBXN010000290 DAEBXN010000000
VERSION     DAEBXN010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.295"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..132
                     /locus_tag="KJL99_004505"
     CDS             <1..132
                     /locus_tag="KJL99_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HBD4438960.1"
                     /translation="LKVDPLKCELRGGQMRFTGLKRGYRLAEQVMMHEPLARIRWCG"
     gene            319..556
                     /locus_tag="KJL99_004506"
                     /pseudo
     CDS             319..556
                     /locus_tag="KJL99_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            977..1555
                     /locus_tag="KJL99_004507"
     CDS             977..1555
                     /locus_tag="KJL99_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438961.1"
                     /translation="MKVSFKSLGYIFHDIYNKKHTIDEFNDVVRKAVLSGKINELNAC
                     HKVAIFLAEKDNEITKKDKAKIIDTLTENYSIEFQQLMNISERTLNSSLYITPGESGF
                     VSFVNREGKICHTAYVKSSDNSMTYYHANGSSIDKYITDMCGLICMRHIESTGIIFYM
                     LDEKVLSAIAEFMNEKGWRAAFCSAKNLYKCV"
     gene            complement(1664..1922)
                     /locus_tag="KJL99_004508"
                     /pseudo
     CDS             complement(1664..1922)
                     /locus_tag="KJL99_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1906..2132
                     /locus_tag="KJL99_004509"
                     /pseudo
     CDS             1906..2132
                     /locus_tag="KJL99_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343765.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          669 a          378 c          523 g          562 t
ORIGIN      
        1 ctgaaggttg acccgctgaa gtgcgagctg cgcggaggtc agatgcggtt tacggggctg
       61 aagaggggtt accgtctggc agagcaggtt atgatgcatg agccgctggc tcgaatacgg
      121 tggtgcggct gagagtcgca gaggggaagt tgcgtccatg ttgaggggaa cggagcaaaa
      181 aaccatcaga gatacattga atcaatcaat gtaagcaata ttaagtgtct ataccgatta
      241 cttgatgatc aggttgtgtt gaataaacgc gcattcttcg gtgctttttt aatcttttgg
      301 ggatttaaat tcctatcgat gaactgtcga tatgaaaaag cgagcatcat actgacgtca
      361 aacaaagggg tcgcagactg gggagaaatg ttcggagatc acgtgctggc aacagcgatc
      421 ctagatcggc tgctacacgc tgaatatcaa aggagagagt taccggttaa aagagaaacg
      481 taaagctgga gtgctgacca aaaacacaac gccaatcagt gatgatgaaa tggtggaaaa
      541 cggacagcat cagtaacgaa agtatcttag caggcatgaa aatcgtccgc atcccctaaa
      601 cggtcaaaat gcttctgccg tgaaggaggg agaatgccat ctcatcactt atatctggcg
      661 aatgaaacac agatggtcgg ccttgccccc aaacacacgg cggtgcaaaa tggaatggca
      721 gaaaacttcg tcaaaacgat gaaatatgat taaatcagtg ttatgttcga acctgacgtg
      781 ttaacagcag taaagtagtc tcgcgaaggt gttcaaacat tataatgaat ggcatcctca
      841 tagtgcgcag ggttatcgct tgctgcggaa atatctgaag cgacgtacca gtaatgggga
      901 aaatgatagc tggtttctga aaatatagag gaaaatgtga tatatatatc ctgttaatat
      961 ttaaggagaa tataaaatga aagtctcgtt taagtcttta ggctatattt ttcatgatat
     1021 atataataaa aaacacacta tcgatgagtt caacgatgtg gtaagaaaag cagttttatc
     1081 aggtaaaatt aacgagctga atgcatgtca taaagtggca atttttcttg ccgaaaaaga
     1141 caatgagata acaaagaaag acaaagccaa gataatagat acattaactg aaaactattc
     1201 cattgaattt caacaattaa tgaatattag tgagcgcacg ttaaattcat ctttatatat
     1261 tactcctggt gaaagtggtt ttgtcagttt tgttaatcgt gaaggaaaaa tatgtcatac
     1321 tgcatatgta aaatcatcag ataatagcat gacatattac catgcaaatg gtagcagcat
     1381 tgacaaatat attacggata tgtgcggcct tatttgtatg aggcatattg agtctactgg
     1441 cattattttt tatatgcttg atgaaaaagt tttatctgcc attgctgaat ttatgaatga
     1501 aaaaggatgg cgggctgcgt tttgttcagc aaagaactta tataaatgtg tttaatttta
     1561 gaaaaactat ttggtaaaat ccaacaagag tcgagaggtt cgcgttgctt cagcagggcc
     1621 tcagcctgtt caaccagact cagttgcagg tcgtgacgca gatttagtgg tggtggttga
     1681 ttgtcagata ccagccgatg atcttgtagt gcatatcatc agactttgag aagcatatgg
     1741 ttttgcgggt aagccgctta agatgtgtcc gcaggttcag attatgtcgc tctatccgct
     1801 gagtgcattt cttgcttata acatgactgg ttgaggacaa taaagttcgg tataccggct
     1861 caggcatcgg tcatgtagaa ggcaatgcta aactggctca gcaaactaag agcagcgcgc
     1921 atccacttac ccaatgcggt tgagaaaata gccttcgatc gctttcatgt ggcgaaacaa
     1981 ccgggtgagg tggtggataa aacccgccag aatgaacatc cgcatcttcc tgttgaaagt
     2041 cgtcgtcagg caaagggaac tcgcttcctg tggcagcaca gcgataaatg gatgaccgaa
     2101 tccaggcagg aaaagctgat atggctgcgt gc
//
LOCUS       DAEBXN010000291         2097 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.296, whole genome shotgun sequence.
ACCESSION   DAEBXN010000291 DAEBXN010000000
VERSION     DAEBXN010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.296"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(724..1713)
                     /locus_tag="KJL99_004510"
     CDS             complement(724..1713)
                     /locus_tag="KJL99_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HBD4438962.1"
                     /translation="MKNPDLQNLTDYSPSDAPWDAHRSVSDDVGGIYLLAAEYERYGA
                     RMALCGGLLRFGWSTLKETGETRLRLREAHFCRVRHCPVCQWRRSLMWQARFYQSLPR
                     IVADYPDARWMFLTLTVRNCAIGELGEMLNRMNAAFQRMKVRKEFRPVQGWIRTTEVT
                     RGSDGSAHPHFHTLMMVPPGMLNGKSYVRHERWVELWRECLRVDYDPNVDVRAVKPRK
                     PKGGESLACATAELVRGAVAETLKYSTKPADMVADPEWFLELTRQTHKRRFVATGGAL
                     KDVLKLDQETDADMVIGDDISEGDDDGSRIAFEWKTEVRKYRRSPTKDKTESG"
BASE COUNT          488 a          645 c          519 g          445 t
ORIGIN      
        1 aaaaaaaacc gtcttgcagg tggttggcct cactgcgtga caccaaccac catccccagt
       61 gtttttcccg tgccgtcgtc aatcacaaaa tcggaaccca cgccggaggc gcgggccccc
      121 gcgattttct gtttgacgaa tcagggaacg gtgaacggta cgcggaattt tttcgcgttc
      181 cgtgaggcgt tggcggggtg tcggggcgca gtcctgacca ggttcgtaga accgcacagg
      241 gttgcaaagg ggtgtcccct ttggcacaag cgacggagcg aaacgccccg ttttgtgtac
      301 gccgcttatc gtgcctgtgt tttaggagaa caaaaaaaat gccgtgcata cagcccacac
      361 aatgacccac ataatcaccc acaactcaaa acacacagcc cacacaatca cccacacttt
      421 aacccacaaa aaagaccaca accccattga caccccacaa aacaacccac acaaacggcg
      481 agtaaatcat tttgccgttt tcgtgccgca gccgttccgc gcaatcgaca aactggcagt
      541 cagggaatct cacgaagtgc gcacttatac aaactcacct taggttcgtt tgtcgtgcgg
      601 tctgcgacgc tcaaaaaacc gagaaacggc gggaattttc gggacaggat gtgtaattgt
      661 aataccgtca cacgcgacgc tatcacaatt gccatctggt caggggtgca ccccgacacc
      721 ccgctagcct gattcagttt tatctttcgt cggtgagcga cggtactttc tgacctcggt
      781 tttccactcg aacgcgattc gtgaaccgtc atcgtcgccc tcggaaatat catcaccgat
      841 caccatgtcg gcgtcagttt cctgatcgag tttcagcacg tctttgagtg cgccgccggt
      901 cgccacaaag cggcgcttgt gcgtctggcg ggtcagttcg aggaaccatt caggatcggc
      961 caccatgtca gccggcttcg tggaatattt cagggtttcg gccaccgcac cgcgcaccag
     1021 ttcggcggtc gcgcaggcca gcgattcgcc gcccttcggc ttacgaggtt tcactgcccg
     1081 gacatcaaca ttcgggtcat aatcgacccg cagacactcc cgccagagtt caacccagcg
     1141 ttcatgcctg acgtaacttt tcccatttag catgccaggc ggcaccatca tcagggtgtg
     1201 gaagtgcggg tgtgcgctgc cgtcgctccc gcgggtgact tccgtggtac gtatccagcc
     1261 ctgcacaggc cggaactcct tgcgaacctt catgcgctga aaagctgcat tcatccggtt
     1321 cagcatctcg cccagctccc cgatcgcaca gttgcgcacg gtcagagtca ggaacatcca
     1381 gcgggcatcg ggataatccg ccacgatacg cggcagggac tggtagaaac gggcttgcca
     1441 catcagggag cgcctccact ggcagacagg acaatgccgg acacggcaga aatgcgcctc
     1501 acgcagcctc agacgcgttt ctccggtttc cttgagggta gaccacccga aacgcagtaa
     1561 accaccacag agggccatac gcgccccgta gcgctcatat tcggctgcaa gcaggtaaat
     1621 ccctcccacg tcatcagaaa cgcttctgtg agcgtcccag ggcgcatctg agggggaata
     1681 gtccgtgagg ttctggagat cagggttttt catgcgcacc acccgatagt gggcgatggc
     1741 tcatctgacg tgttatcatt gccatatcac ctgttgtgtt agtcgtatca ccaattcgaa
     1801 ttcctacagg tggtcacacc cccgaacgat tgccctcgtt gcgggggtcg cttttaatgc
     1861 gaccactccc gacagaataa ctccaaaacc attttccgac aaatccccct tcaggccttg
     1921 cggcacaagg gctggaggcg aatttaaatc ctagagttat cccttaagta taaatacaag
     1981 gatcgcgcgc gagattcgct tgaattttga gcgggatttt cagtcaccca aaacacccaa
     2041 ccccacccac ttacatccat ctcatccaca gactttcacc ccgattttgt gaacaac
//
LOCUS       DAEBXN010000292         2089 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.297, whole genome shotgun sequence.
ACCESSION   DAEBXN010000292 DAEBXN010000000
VERSION     DAEBXN010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.297"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(60..1025)
                     /locus_tag="KJL99_004511"
     CDS             complement(60..1025)
                     /locus_tag="KJL99_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HBD4438963.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRM
                     ASCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIV
                     VDYPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRG
                     KDGSAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGE
                     VVANVAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQL
                     DRETNEDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
     gene            complement(1879..>2089)
                     /locus_tag="KJL99_004512"
     CDS             complement(1879..>2089)
                     /locus_tag="KJL99_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438964.1"
                     /translation="RSEAVIPDRFKNLILLIDGLSFGTFGFHPHEGFEDELILYIQKT
                     NERVKTLFVRIDLNGIKFGFLRTHS"
BASE COUNT          577 a          551 c          436 g          525 t
ORIGIN      
        1 aaaaaaaata aaccaatagt cttatttcca ttcaggtatt caatattaat attcatacgt
       61 taatccgatt tatccttctc aggggcgcgt ttgtaacgcc gtttacctga atcccagaca
      121 aacgccgtcc gcttcccgtc atcagtgcca tcccctacat cgtcggcaat gacaagatcc
      181 tcattggttt ctcgatccaa ctggaggacg ttttttagcg ccccaccggt cgagataaaa
      241 cggcgcttgt gaagctgccg cgtcagctca agaaaccact caggatcgtt tgccatatct
      301 tccggtttaa cggagtattt cagcgtttca gcaaccgcgc tttgcagttg ctcggcaacg
      361 ttggccacaa cctcacctgt cttagttttt actgcccgaa tatcgatatt cggctcatag
      421 ttcacccgca agcaatcgcg ccagagttct acccaacgtt cgtgcttaac gtagttcttc
      481 cctttaaacc aagaaggttg caccatcagc agacagtgaa aatgcggatg tgcgctgcca
      541 tccttacctc gcgtcacctc cgtagccctg atccacccct gaacaggtga tagctccttt
      601 cgcttttcca ttcgcttaaa cgccgcattc attgctgtaa ggactgttcc aagttcacct
      661 atctcgcagt tcctgacagt taacgtcaga aacaaccatc gggaagacgg gtaatccaca
      721 acgattttcg gtagagcctg ataaaaacgg gcttgccaca tgagggttct tctccactgg
      781 cagacagggc aatgacggac acggcaaaat tgcgcactac gaagccgtag gcgcgtttct
      841 ccggtttcca tgatggtaga ccaaccaaaa cgtaacaaac cgctacagga ggccatacgg
      901 agtgcataac gctcgaattc agcagcagtc tggtagatac ccccgacaga atcggtaagc
      961 gtgcgctggg tatcccaaac tgcatcacgg gggctgtagt ccgaaaggaa tttatcttct
     1021 gacattaagc ctgctccgat ctgggcaggc tgtgatttga tgtggtatat tcatcacgta
     1081 cctgtgcatt gcgagccagt acatgtcacc agagtttgcc gctgaggtga cacattcgaa
     1141 atgttgggga gtgttgacgc actccccaac gccgattaaa aacgcctcaa ttctgctcca
     1201 ttttctttga cctgtcaaaa cccactcaaa gcctcgtcat tactgggcta aaccactatt
     1261 tcatacccgc catttatccc ttaagtataa atacaaggat cacgcgcgag aattgcgcaa
     1321 aaaataacca caattttaac cccccaaaac aagcaccccc gaacggctta ccccaacctt
     1381 ttccacacac ccgatcacca aaacagggga taaaaacaaa accctgtctt acaggtggat
     1441 ggcctcactt cgtgaaacca tccaccccgc ataagttttt taatcggtgg tgtcgtcaat
     1501 cactaaatcg gaaccccaaa ggggaccccc acgatttagc atttgacgag attaactctc
     1561 ggagatggca tcatgctttt aacggacttt aaaaaacggt gaacggatgc ggattttaat
     1621 ccgtgtctgt gaggcgttga gcaacgagca aagcgaggcg gtagaaagag agccagcggc
     1681 aggctcagga cgataatccg gggaaaatgt accactcgca gagtgttaac atttgacatc
     1741 ggattgtcta tcctgcattg gtgtcgagtt ggcaaggctg aggataatgc tgaggataat
     1801 gcaggataga cagagcacta gagattgcat gcaatctcgt acctatcacc tcagcctgcc
     1861 gctggctctt ttccagaact aagaatgcgt tcttaaaaat ccaaacttga taccgtttaa
     1921 atctattctc acaaaaagag tttttaccct ctcgtttgtt ttctgaatat ataaaattaa
     1981 ttcatcctca aaaccttcgt gcggatgaaa accaaatgtg ccaaaactta atccatcaat
     2041 aagcaaaata aggtttttaa acctgtcagg aattaccgct tcacttctt
//
LOCUS       DAEBXN010000293         2084 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.298, whole genome shotgun sequence.
ACCESSION   DAEBXN010000293 DAEBXN010000000
VERSION     DAEBXN010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.298"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            1..796
                     /locus_tag="KJL99_004513"
                     /pseudo
     CDS             1..796
                     /locus_tag="KJL99_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608690.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            911..1252
                     /locus_tag="KJL99_004514"
                     /pseudo
     CDS             911..1252
                     /locus_tag="KJL99_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            1235..2072
                     /locus_tag="KJL99_004515"
                     /pseudo
     CDS             1235..2072
                     /locus_tag="KJL99_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          515 a          504 c          631 g          434 t
ORIGIN      
        1 gggccttgcc cgtagcagtt atttttatca ccgggctgca ctgaaagccg gtgataaata
       61 cgccacgata cgtaagatgc tgacagatat ctttaacagt aattaccagt gttatggcta
      121 tcgtcgcctg catgcgatgc tcaggcatga gggggggcgg ctatcagaaa aggttgtacg
      181 cagacttatg gtggaggaac agcttgtcgt cagtcgtaac cgtcgtcgtc gctacagctc
      241 atattgcgga gaaatcggac cggctccgga taaccttatc gccagagatt ttaaggcgga
      301 gcaacctaat cagaaatagc tgacagatat cacggagttc cagctccctg caggtaaagt
      361 ctggctgtca ccagtggtgg actgcttcga tggaaaagtt gtgagctggt ctctcagtac
      421 acgccctgat gctgaactgg tcaacactat gctggatagc gctgtcgaaa cgttaaatgc
      481 tggcgaacga ccggtgatac acagtgacag aggtgggcat tatcgctggc caggctggct
      541 ggaaagagtg aatgcagcag gtcttattcg ctcaatgtcc cgtaaaggat gttcacctga
      601 taatgctgca tgcgaaggct ttttcggcag actgaaaacg gaaatgtatt atgggcgtaa
      661 atggtcgggc atcacgccag aaaagttcat gcagcaagtg gatgcttaca tcagatggta
      721 taacgagcgg cgtataaaat tatcgctggg tgccgtcagc cccaaaatgt accgccaaca
      781 atgcgggctg gaatgataaa gcagtccagg aaatcgtccg catcccccct tcaccatttg
      841 gattgtccgc tccacccaac atgttgtttc cttaaggttc ctacaccaca aaggaaactc
      901 tcatgctgcg cagtggatgg ctgacgttgc cgacaggcgg gaacttcgcc agttcaggca
      961 gacaccggaa cagcgcttca cgcaggaaca ggagcatctg cagccgctgc tgggtacgga
     1021 cttcgatatc cgtcatgtct cctgggatgg ctatatcgag gttggcggga accgttacag
     1081 tgtgccggaa agcctgtgtg gtcagctggt atcaatacgg atctcgctgg atgatgaact
     1141 gcggatctac agtaatgagc aacaggtagc atcgcaccgt ctctgttcgg ctgcatacgg
     1201 ctggcagacc gtgccggagc atcacgctcc actctggcag caggcttcgg agcgcatggc
     1261 cgaatggctg ggggagatac agaaacgggt aatcactgtc tgtgaccggg aagcggatat
     1321 ctggcattac ctgtattata aagtcagcca cggacagcgt ggtgcgtgct gcacagaatc
     1381 gccggctgga agaggcaccc ggcaagctct tcgaactgcc ggaagtcctg gcaaccgcag
     1441 gaagccacac gctgaatgtg atgcaaaaag gcgggcgggc agcccgtcag gcccggatgt
     1501 tcatcagtta cagcgaagtc agcataaaaa atcccgacaa cagcggccag gcgctcccgc
     1561 tcacgtatgt ctgttgccgg gagcaggcag aggacggtgc ctgctggcat ctgctgacgt
     1621 caggaaaagc ggcgagtgcc gccgatgcac gacgtattgt cagccattac gagcgacgct
     1681 ggctgacaga ggaataccac aaggcgtgga aaagtggtgg tacatggaat cgctgcgaat
     1741 gcagacccgg gataaccttg agcgcatggt ggttatcctg gcgtttatag cggtacgggt
     1801 gctgggcctg cggcagggag gtgtcagtga agaaacacaa aatgacagct gtgagaaaat
     1861 actgacgccg acagagtgga aactactgtg ggttaagctg gaaggaaagc cgcttccggt
     1921 gcaggcaccg accctgaaat gggcctgggg cgatggcatg acagcaaacg cacaggtcgt
     1981 cccggttggg gcgtcatgtg ggatggctgg tcagacttca ggatatggtt gagggttacc
     2041 tggtgatgaa gtctcttgat caggagctct gatcaagaga cagc
//
LOCUS       DAEBXN010000294         2083 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.299, whole genome shotgun sequence.
ACCESSION   DAEBXN010000294 DAEBXN010000000
VERSION     DAEBXN010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.299"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(66..611)
                     /locus_tag="KJL99_004516"
                     /pseudo
     CDS             complement(66..611)
                     /locus_tag="KJL99_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338841.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1273..>2083)
                     /locus_tag="KJL99_004517"
     CDS             complement(1273..>2083)
                     /locus_tag="KJL99_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438965.1"
                     /translation="NIPFPVEVLPTRNSLPTPIIAHTLADRSVVVPVRGGKRPEVVTA
                     PSGTEITVEPIEQAILVSHQTKLWDQKSSTGFTQGTLRQDALNICDNVVRTINSKMVD
                     VLESSKLLKTVELPVLTGSLTAKADAIMDALYENTESSFGSEVTDYGIIAHESHLKAL
                     SRLAAKQGFGGEDAIVDMLGTDIAYYNGVDRGVFMMAKRFTALSFGCFRHDGENITVV
                     LSRDGNTQSHDLEILGKVFVVAEAATTIKMGTGSAAAVLPVVKRLSFTKTS"
BASE COUNT          570 a          471 c          498 g          544 t
ORIGIN      
        1 tccggaacaa tttgaaaacc agaacctcgc ttaggcctgt gtccatatta cgtgggtagg
       61 atcagaattc catccagtcg ttttgggttg ctaatgatgt cctgcatccg cttaatctgg
      121 tcgggcgtag acatataacc ccacagggtg agcagaacct tttttgcggc tacactattt
      181 ccttggtctg atactaatgc catatagccc cataaactat ccctcagcca gacagcatca
      241 tagtgggttt ctgccgctgt tttgtcatct tcgaaagtag aagcaagaat aaacgttccc
      301 cagggtttct ccgtcaacgt taactttcgt gtgttttctg ttaatgttag caatccttga
      361 gtttcgtctt tggtatagtg actctttatt tcatttttta atttggctga gtgaatcaga
      421 agacgataat taggatactt atctggattt aacgataaat aacttttatc tgttgctgcc
      481 cccctggaat taagaatgtt aaatacagat tcgcttgtca cttctgcctc tgtcgcctgg
      541 catgtgccgc acgaaaatat accagcgagc agaagagtta atgcagattt ccctttcata
      601 aaataatcca tcaatcgttt gttaaaagcg tagataacca cttattcaac aacttgaaaa
      661 taaggtattt tacctgagtt gttacaaaag gattgcattg cgtaaacgct ttttatttac
      721 aaaaaaatgg ggaagtgtta cagcgattgc gaaaagcctg aagtgattga attaagataa
      781 tatagaaaat cagatacagc aaaaatggca gattgatcag gtgaagataa attggagcgg
      841 gcgaagggag ccgaggacaa ctagctaact tcttgtttct taaagactga tgaatccagt
      901 ggttacagtg atggttacac atcgagaggc tgacggtcaa gacttgaagc acgtaatatt
      961 cccataaaag gaatagaaca cagtcactga ctacccggcc ctttggtctg actgagggtc
     1021 gggctatggg gtaaatcgtc aggcttattg tattagatgg tctctcacat ttttcagtca
     1081 aaaactcatc gtactttagg ccagaccatc agcacgacta ccagagctag ctttagcagg
     1141 aagaccacga gatagacttt gtgtaggtcc attaggtcct cctctttccg tacccatgaa
     1201 ctagattgct agtacaaaat aaaggccaga catccagtta cggaagtctg accaataagt
     1261 cgttatcgtt agttagcttg tcttagtaaa gctcaggcgt ttaactacag gcaggacagc
     1321 agcagcgcta ccagtcccca tcttgattgt cgttgcagcc tcagcgacca cgaagacctt
     1381 accgagaatc tcaaggtcgt gagactgggt gttgccatct cgggacagga ccacagtaat
     1441 gttctcgcca tcgtgacgga agcaaccgaa gctcagagcc gtgaagcgct tagccatcat
     1501 gaatacacca cggtctacac cgttgtagta ggcgatgtct gtccccagca tatcgacgat
     1561 agcgtcttca ccaccgaagc cctgcttagc agcaagacgg gagagagcct tgaggtgtga
     1621 ctcatgggcg atgataccgt aatcagtaac ctcagaaccg aaagaagact cggtgttctc
     1681 gtacagggca tccatgatag cgtctgcttt ggccgtcagg ctcccggtca ggacaggaag
     1741 ctcgacggtc ttcagcagct tagaggactc cagaacgtca accattttag agttaatggt
     1801 acggaccacg ttatcgcaga tgttcagagc gtcctgccgg agcgtcccct gagtgaaacc
     1861 agtagaggac ttctggtccc ataacttggt ctggtgggac acgaggatag cttgctcaat
     1921 cggctcgacg gtgatttcag taccagacgg agcggtcacg acttcaggtc gcttaccacc
     1981 acggacagga acgaccacag agcggtcagc cagagtgtga gcaattattg gcgtcggtaa
     2041 gctgttacgg gtcggcagga cttctacagg gaacggaatg ttt
//
LOCUS       DAEBXN010000295         2066 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.300, whole genome shotgun sequence.
ACCESSION   DAEBXN010000295 DAEBXN010000000
VERSION     DAEBXN010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.300"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(<1..374)
                     /locus_tag="KJL99_004518"
     CDS             complement(<1..374)
                     /locus_tag="KJL99_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HBD4438966.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYLSSRRNSKAAYRFLGKILN"
     gene            complement(544..825)
                     /locus_tag="KJL99_004519"
                     /pseudo
     CDS             complement(544..825)
                     /locus_tag="KJL99_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
     gene            complement(972..1832)
                     /locus_tag="KJL99_004520"
     CDS             complement(972..1832)
                     /locus_tag="KJL99_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A broad-spectrum beta-lactamase TEM-1"
                     /protein_id="HBD4438967.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
BASE COUNT          550 a          539 c          475 g          502 t
ORIGIN      
        1 ttgttgagga ttttacccag aaaccggtat gcagctttgc tgttacgacg ggaggagaga
       61 taaaaatcga cagtgcggcc ccggctgtcg acggcccggt acagatacgc ccagcggcca
      121 ttgaccttca cgtaggtttc atccatgtgc cacgggcaaa gatcggaagg gttacgccag
      181 taccagcgca gccgtttttc catttcaggc gcataacgct gaacccagcg gtaaatcgtg
      241 gagtgatcga cattcactcc gcgttcagcc agcatctcct gcagctcacg gtaactgatg
      301 ccgtatttgc agtaccagcg tacggcccac agaatgatgt cacgctgaaa atgccggcct
      361 ttgaatgggt tcatgtgcag ctccatcagc aaaaggggat gataagttta tcaccaccga
      421 ctatttgcaa cagtgccata tcaagcgact tctcctatcc cctgggaaca catcaatctc
      481 accggagaat atcgctggcc aaagccttag cgtaggattc cgccccttcc cgcaaacgac
      541 cccaaacagg aaacgcagct gaaacgggaa gctcaacacc cactgacgca tgggttgttc
      601 aggcagtact tcatcaacca gcaaggcggc actttcggcc atccgccgcg ccccacagct
      661 cgggcagaaa ccgcgacgct tacagctgaa agcgaccagg tgctcggcgt ggcaagactc
      721 gcagcgaacc cgtagaaagc catgctccag ccgcccgcat tggagaaatt cttcaaattc
      781 ccgttgcaca tagcccggca attcctttcc ctgctctgcc ataaggggtc tgacgctcag
      841 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
      901 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
      961 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     1021 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     1081 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     1141 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     1201 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     1261 agtttgcgca acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt
     1321 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     1381 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggcagca
     1441 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     1501 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     1561 cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcaccaca tagcagaact
     1621 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     1681 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     1741 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     1801 ataagggcga cacgaaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     1861 atttaccagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     1921 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
     1981 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tcttcaagaa
     2041 ttttataaac cgtggagcgg gcaata
//
LOCUS       DAEBXN010000296         2060 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.301, whole genome shotgun sequence.
ACCESSION   DAEBXN010000296 DAEBXN010000000
VERSION     DAEBXN010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.301"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            1..2060
                     /gene="sat"
                     /locus_tag="KJL99_004521"
                     /pseudo
     CDS             1..2060
                     /gene="sat"
                     /locus_tag="KJL99_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
BASE COUNT          753 a          362 c          409 g          536 t
ORIGIN      
        1 ggagattcag ggggccagtc tactcatacg gcatgatgat ttataaaaat gataaaacat
       61 ttcgtaatct tgaaatattc ggagacagcg gctctggagc atacttatat gataacaaac
      121 tagaaaaatg ggtattagtc ggaacaaccc atggtattgc cagcgttaat ggtgaccaac
      181 tgacatggat aacaaaatac aatgataaac tggttagtga gttaaaagat acctatagtc
      241 ataaaataaa tctgaatggc aataatgtaa ccattaaaaa cacagatata acattacacc
      301 aaaacaatgc agataccact ggtactcaag aaaaaataac taaagacaaa gatattgtgt
      361 tcacaaatgg gggaaatgtt ctgtttaagg ataatttgga ttttggttgc ggtggtatta
      421 tctttgacga aggccatgaa tataacataa acggtcagag atttacattt aaaggagcag
      481 gaattgatat cggaaaagaa agcattgtaa actggaatgc attgtattcc agtgatgatg
      541 ttttacacaa aataggcccc ggtactctga atgttcaaaa aaaacagggg gcaaatataa
      601 agataggtga aggaaatgtt attcttaatg aagaaggaac atttaacaat atataccttg
      661 caagcggaaa tggtaaggta atactaaata aagataattc ccttggcaat gatcaatatg
      721 cggggatatt ttttactaaa cgtggtggca cgctagattt aaatggacac aatcagactt
      781 ttactagaat tgccgccact gacgatggaa caacaataac taactcagat acaaagaaag
      841 aagccgttct ggcaatcaat aacgaagact cctacatata tcatgggaac ataaatggca
      901 atataaaact aacgcacaat attaattctc aggataagaa aactaatgca aaattaattc
      961 tggatggtag tgtcaacaca aaaaatgatg ttgaagtcag taatgccagt cttaccatgt
     1021 aaggccatgc aacagagcat gcaatattca gaagcacagc gagtcattgc tccctggtat
     1081 ttctttgtgg aacggactgg gtcaccgttt tgaaagaaac agagagttca tataataaaa
     1141 agttcaattc tgattacaaa agtaataatc agcagacctc atttgatcag cctgactgga
     1201 aaaccggggt gtttaaattt gatacattac acctgaacaa tgctgacttt tcaatatcac
     1261 gcaatgccaa tgttgaagga aatatatcag caaataaatc agctatcaca atcggcgata
     1321 aaaatgctta cattgataat cttgcaggga aaaatattac taataatggt tttgacttca
     1381 aacaaactat cagtactaat ctatccatag gagaaactaa atttacaggt ggcatcactg
     1441 cacataacag ccaaatagcc ataggtgatc aagctgtagt tacacttaat ggtgcaacct
     1501 ttctgaataa tactcctata agtatagata aaggagcaaa agttatagca caaaattcca
     1561 tgttcacaac aaaaggtatt gatatctccg gtgaactgac tatgatggga atccctgaac
     1621 agaatagtaa aactgtaacg ccgggtctcc actacgctgc tgatggattc aggctgagtg
     1681 gtggaaatgc aaatttcatt gccagaaata tggcatctgt caccggaaat atttatgctg
     1741 atgatgcagc aaccattact ctgggacagc ctgaaactga aacaccgact atatcgtctg
     1801 cttatcaggc atgggcagag actcttttgt atggctttga taccgcctat cgaggcgcaa
     1861 taacagcccc caaagctaca gttagcatga ataatgcgat ctggcatcta aatagccagt
     1921 catcaattaa tcgtctagaa acaaaagaca gtatggtgcg ttttactggt gacaatggga
     1981 agtttacaac ccttacagtg gacaacctta ctatagatga cagtgcattt gtgctgcgtg
     2041 caaatctggc ccaagcagat
//
LOCUS       DAEBXN010000297         2029 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.302, whole genome shotgun sequence.
ACCESSION   DAEBXN010000297 DAEBXN010000000
VERSION     DAEBXN010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.302"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(<1..1824)
                     /locus_tag="KJL99_004522"
     CDS             complement(<1..1824)
                     /locus_tag="KJL99_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HBD4438968.1"
                     /translation="MRPSELSDLLWAQVDRVAPHLLPNGKIEGHEWVAGNVNGDKGNS
                     LKVNLIGKKKWADFAEGDGGDMLDLWMACRGINLHQAMQEAKAFLGIKDDDHHFDARR
                     EKKFSRPDRKKIARYVTRTESHLEYLQSRGISPEVVKRYEVVSGKVWNGERELDALVL
                     PYKRDGELLQVKRISTERPDGKKVIMAEGDCEPCLFGWQALDAGVRVVVLCEGEIDCM
                     SYAQYGISALSVPFGGGKGAKQQWIEFEYHNLDRFEEIFISMDVDDVGREAAREIVSR
                     LGEHRCRLVTLPYKDINECLMNGVTEDEIWQYIGTASYFDPEELYSAREFYQDTINAF
                     YGKQQYLFNPPWESLADKFQFREAELTLVNGVHGHGKACPLNEPILLADGTWTTHGNV
                     KIGDQVASVDGNPSTVTGIFPQGVRDVYRVTFEDGRYVDCAGDHLWEVTSRGFTKGEK
                     RRVIDTFGLKRLSETKRHKNGVRIPEITGDFGDHSEPLAWVIGSLLGDGSLSNGSVKF
                     SNVEPYMIERMKAELPDYNFSGDGKDWLISTARGQVNPLMETLRGYGLMGCTAKNKFI
                     PRVFFSANKSTRIGMLCGLLETDGYVEKDGTLVFSSASEELR"
     gene            complement(1932..>2029)
                     /locus_tag="KJL99_004523"
     CDS             complement(1932..>2029)
                     /locus_tag="KJL99_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBD4438969.1"
                     /translation="WNDEKPETIQPQSRPSSAITVSKTGYVFFDR"
BASE COUNT          485 a          579 c          430 g          535 t
ORIGIN      
        1 gcgcagttct tcacttgctg aggaaaaaac aagcgttcca tccttctcga catacccatc
       61 cgtttcaagc agaccacaca gcatgcctat acgcgttgat ttatttgcgg aaaaaaacac
      121 acgagggatg aatttgtttt ttgctgtgca ccccattagt ccataacctc gcagggtctc
      181 catgagtgga tttacctgac cacgcgccgt tgatatcagc cagtccttac catctccaga
      241 gaagttgtaa tcaggcagtt cagccttcat acgctcgatc atgtatggct cgacgtttga
      301 aaacttcacg ctcccattgc taagactacc atccccgaga agggagccga taacccatgc
      361 taatggctct gagtggtcgc caaagtcacc agttatttca ggaatcctaa cgccattttt
      421 gtgcctcttc gtttcactca accgcttcaa cccgaaggta tctatcacgc ggcgtttctc
      481 gcctttcgtg aatccacgac tagtgacctc ccataggtga tcgcctgcgc aatcaacata
      541 acgaccatct tcaaatgtga ctcggtaaac atctctaaca ccctgcggga atatcccagt
      601 gacagttgac ggattgccgt ctactgacgc cacctgatcg ccaattttta cattcccgtg
      661 agtagtccat gtcccatcag ctaaaagaat aggctcattc agtgggcatg cctttccgtg
      721 accgtgcaca ccattgacca gcgtcaactc tgcctcacgg aactggaatt tatctgccag
      781 agattcccac ggtggattaa acagatactg ctgcttgccg tagaaagcgt tgatagtgtc
      841 ctggtaaaac tctcgcgcac tgtagagttc ttcaggatcg aagtaggatg ccgtgccgat
      901 gtactgccag atttcatcct cggtaacacc gttcatcagg cattcgttga tgtctttgta
      961 cggcagagta acaagacggc aacgatgttc accgagtcgg cttacgattt cccttgcggc
     1021 ttcacgacca acatcatcaa cgtccatcga gatgaatatt tcctcaaacc tgtcgaggtt
     1081 gtgatactca aactcaatcc actgttgctt agcgcctttc ccgccaccaa acggcacgga
     1141 taacgccgag atgccgtatt gcgcatagct catacaatca atttcgcctt cgcaaagcac
     1201 aaccaccctc acgccagcgt ccagagcctg ccatccgaac aggcaaggtt cgcaatcacc
     1261 ttctgccata atgactttct tcccgtccgg gcgctcagtg ctgattcgct tgacctgcaa
     1321 caactcacca tcgcgtttgt acggaagcac cagagcatcc agttctcgtt ctccattcca
     1381 caccttgccg ctgacaacct cgtagcgctt tacgacttct ggcgatatgc cacgcgattg
     1441 caggtactca agatgggatt ctgttctggt aacgtagcgg gcgattttct tacgatcagg
     1501 tctggagaat ttcttctcac gtctggcatc gaaatggtga tcgtcatcct tgataccgag
     1561 aaatgctttc gcttcctgca tagcctgatg caggttaatt ccacgacatg ccatccacaa
     1621 atcaagcatg tcaccgccgt ctccctcagc gaaatcagcc cattttttct tgccgataag
     1681 gttgacctta aggctgtttc ccttgtcacc gttgacgtta ccggcaaccc actcatgccc
     1741 ctctatcttg ccgtttggca acaggtgcgg agccaccctg tcaacctgcg cccaaagcag
     1801 gtcgctaagt tcacttggcc tcatgattcc ctcagattga gatttttaaa ccagaaatcg
     1861 acaaacgaaa tacttaacca gccgtggtta taaccagcga ccagtagcga tttgattttt
     1921 gatttcatgg ttcacctgtc gaaaaacaca tagccagttt tcgatacggt gattgcggat
     1981 gatggtctgg attgtggttg aatggtttct ggcttctcgt cgttccagc
//
LOCUS       DAEBXN010000298         2021 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.303, whole genome shotgun sequence.
ACCESSION   DAEBXN010000298 DAEBXN010000000
VERSION     DAEBXN010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.303"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(81..1493)
                     /gene="pykF"
                     /locus_tag="KJL99_004524"
     CDS             complement(81..1493)
                     /gene="pykF"
                     /locus_tag="KJL99_004524"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HBD4438970.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEKGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT          509 a          455 c          489 g          568 t
ORIGIN      
        1 tattgcttct ggttatcgat taaataaaaa aagcgcccat cagggcgctt cgatatacaa
       61 attaattcac aaaagcaata ttacaggacg tgaacagatg cggtgttagt agtgccgctc
      121 ggtaccagtg caccagaaac cataactacg acgtcacctt tgtgtgccag accgctctgc
      181 agagccagtt ctttacccag acggtagaaa tcatcagtag aagtgatctc tttaaccagc
      241 tgcggcacaa cgcctttgct cagtaccaac tgatgagccg ttttttcgtt ggtggtcagt
      301 gccaggatgg tggcatccgg gaagtattta cgtactgcgc gagcagattt accgccctgg
      361 gtagcaacca cgatcagcgg agcatccagt ttttcagcag tttcaacggc accacggcat
      421 accgcttcgg tgatgcgcag tttacggttg tcattgttga actcgagacg gctgttcatc
      481 acgcggtcgg tacgttcgca gatggtcgcc atgatagaaa ccgcttccag cgggtattta
      541 ccttttgcgg attcaccaga cagcatcact gcgtcagtac cgtcgaggat ggcgtttgca
      601 acgtcaccgg cttctgcgcg agtcgggcgt gggtttttga tcatggaatc cagcatctgg
      661 gtcgcagtga taacgacttt acgtgcacgg atacattttt cgatcatcat cttctgggcg
      721 aagataactt cttctaccgg gatttctaca cccaggtcgc cacgcgcaac catgatgccg
      781 tcagaggctt cgaggatttc gtcgaagttg ttgaggcctt cctggttttc gattttggag
      841 atgatgtgga tgttttcgcc gccgtgcgct ttcaggtgct cacggatttc gataacgtca
      901 gaacgcttac gaataaagga agcagcaaca aagtctacgc ctttttcgca accaaagatc
      961 aggtcctgtt tgtctttttc agccagtgct ggcagagcaa tggaaacgcc aggcaggttc
     1021 acacctttgt tttcgcccag gtcaccgttg ttcagcactt tacagataac tttgttacct
     1081 tcaatggcgg taacttccat accgatcaga ccatcgtcaa ccagtacggt gttgccaaca
     1141 gacaggtcag tagtgaaacc ttcatacgtt accgcaacca tttcgctgtt gccgataaca
     1201 gatttatcag tggtgaaagt aaaggtctga ccagctttca gagaaacatc gttaccgcct
     1261 tccagtttca tggtgcggat ttccggacct ttggtatcaa gcaggatagc ggcggtttta
     1321 ccagttttgc tcatcacgtt gcgcagattc tgaatgcgct gaccgtgttc tgcatagtca
     1381 ccatgagaga agttcagacg cataacgttc atgccagcgt ccagcatttt agctaacatc
     1441 tcttcagatt cggttttcgg tccgatggtg caaacaattt tggtcttttt catgacagtc
     1501 ttagtcttta agttgagaag gatgggagaa acttgctttc tgggcgcact gccggaaagc
     1561 gaatccggta ttacgaaagt ggtggtgcct cgctctaagg ataggtgaca tcgaaaaagc
     1621 gtgcagagga atgtgtgctt gtgtctcagc ccaacgtgag tgtctgtttt tgagtcgttg
     1681 agttctacag tccaaagtgc tgaaaccatt caagagtcaa ttggcgcgca ttatacgctg
     1741 aaattaatcc aaaaggaaaa caaaaacagc gtttcaaaaa atatacaact tgttagacaa
     1801 taaattgtta tcaaagattt aacgttccag ggaaaaggtt acgcaaaaac ttcgctgttt
     1861 gcgcaacgtc tggtgccgaa gttgtcagtg tgaggataag tgaaagttca tccggtgtat
     1921 aaatatatca tcgctcaatt gctacagtta aaataattta tcgcgttatt tgtctgattt
     1981 tgtttgattt ttgtttaatt ggttagattg atatatgctg a
//
LOCUS       DAEBXN010000299         1976 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.304, whole genome shotgun sequence.
ACCESSION   DAEBXN010000299 DAEBXN010000000
VERSION     DAEBXN010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.304"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(1..837)
                     /locus_tag="KJL99_004525"
                     /pseudo
     CDS             complement(1..837)
                     /locus_tag="KJL99_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
BASE COUNT          533 a          465 c          456 g          522 t
ORIGIN      
        1 agctgctcta cggtcatatt tttaattgcg ctccggttta caagagccca cccctgtttt
       61 tccagataaa actggaaagt atccagggta cagaccagtg caccgtcagg aacagtttcg
      121 gtgaatctga tattgccgtg ttcgtcgaag tgcacaacca gagtgcgacc gttacccgga
      181 atcatcttgt cagcggacgg ggtattattc tggtgcagtt ctgcctccat gcggtcgaac
      241 atcagcgatg taggcctctt tgaatgcagc ggcttttttc ccagtgaagc ccatcaccag
      301 gaaaacgaag ccgtttttgg tgatttggta cattgggcgt ttttcgcctt tggtgtcggt
      361 gtaggtgacg tcctcaaaat tgagggcgtt aaattcaggt gagcattcag tatttgcgat
      421 ggcacgtaac acattgtcgt gtcgtttgtg gaagaactct gcaaccgcaa cagacgtagt
      481 gacagcgcga ccgttttcga tggttacgtc agggtgagaa agggcaggga tagtagccat
      541 gatggcatcc tcgagtgata agttaattaa ctcaccaccg aggttttcca cgaccataag
      601 ggtggtgaga cgtacagggg tggaaatacc ggtcactcga gaacccggcc agtcttgcga
      661 ctgccctgca catcccacca taatttgaat ggagctgtgc attacgcata aaaaaaccgc
      721 ttcagcgcgg ctatgcgctc gagtaacgtt cgggtttcca cgcccggccc ccgttttatg
      781 aggtgcagat gcactataat tccacccgtt ctggttttca atagctacat tcaacatttt
      841 ctcttacctt tcatcaccga agtgaacttt gttgatgcgg tgcctggtgc ctccaggtga
      901 cgttaaccag ttaacaatta acgccggata cagggaaacc cataaccacc cgatacgtgg
      961 gaactaatcg ctttttaact gttccgcgtg cgctcagccg cattcaccgc atcacaaaat
     1021 tcactttaaa aagggcggac atcagccagc aatgaaactg atgccgccaa aggtaccaat
     1081 caacatggag tgttgtggcg tggttgtcac ttaagcgtat ggtcaacctg acaacccggt
     1141 gtcctcaacg gggaaggaat aactccgcca tacttaccgc cgcgtcattt cgcgttgtgt
     1201 gcctgctttt aactacgtca ggcgaggtgg ttcctgttat tccccaacaa caagaaattt
     1261 gtataatctg aataccccaa caataagaag agtgagcaag tgatgtatta gcgatgtcat
     1321 ctctcaaaga gactgcatca atgttaaaat tattcaatga agcaaaaact caggctgagg
     1381 ttgatcgcgc tgtgtctgaa ataaactcta agttgtcaag cattcagctt gaatatttgt
     1441 catctcattg atgagctgac aatctctaaa agcaatgagg ctgcgctcaa agcaaaactc
     1501 acggagatag aaaagtttaa gttccaaagt gaagactgcg aacctcgcta attgattact
     1561 ggaacgattg tgtatgccag acagtgcaat actagctctg gcaagattac catgtatttt
     1621 tgtccagaat gttttgcgaa gagagcaata tctatattgc aacctttccc tatcgaggaa
     1681 ggcgataaat atacgaaaac tcaatgcccc tcctgtaaaa atgagtatct catggactac
     1741 aatttcaata atgatttttg agtcacggta ttcagatttt taaagaacat gccggatact
     1801 cacccgtgtc cggcgcacgc actccacctc acccgtggag aactccttaa ttaccaacct
     1861 tggcttcgtt ggttagccat taacgctggt atgtaatcat tctggcaatg cttaatttga
     1921 agtggcacac tgaatttggc cacctgaaca gaggtgatat gctcacctca gaacaa
//
LOCUS       DAEBXN010000300         1969 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.305, whole genome shotgun sequence.
ACCESSION   DAEBXN010000300 DAEBXN010000000
VERSION     DAEBXN010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.305"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            240..509
                     /locus_tag="KJL99_004526"
                     /pseudo
     CDS             240..509
                     /locus_tag="KJL99_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            506..732
                     /locus_tag="KJL99_004527"
                     /pseudo
     CDS             506..732
                     /locus_tag="KJL99_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            761..1351
                     /gene="ospG"
                     /locus_tag="KJL99_004528"
     CDS             761..1351
                     /gene="ospG"
                     /locus_tag="KJL99_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector protein
                     kinase OspG"
                     /protein_id="HBD4438971.1"
                     /translation="MKITSTIIQTPFPFENNNSHAGIVTEPILGKLIGQGSTAEIFED
                     VNDSSALYKKYDLIGNQYNEILEMAWQESELFNAFYGDEASVVIQYGGDVYLRMLRVP
                     GTPLSDIDTADIPDNIESLYLQLICKLNELSIIHYDLNTGNMLYDKESESLFPIDFRN
                     IYAEYYAATKKDKEIIDRRLQMRTNDFYSLLNRKYL"
     gene            1690..1969
                     /locus_tag="KJL99_004529"
                     /pseudo
     CDS             1690..1969
                     /locus_tag="KJL99_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          560 a          432 c          462 g          515 t
ORIGIN      
        1 ctgaaaacgg ctcacaactg caccattcat aatcacatcg cataaaactg tcagcgccag
       61 tgataggaca ggtgaaacct gtcaatacgt accggcttac gccgggcttc ggcggtttta
      121 ctcctctgcc atatgcaaca acggagtgcc gccttacatg ccgctggcgc ggcatatcct
      181 ggtgacgata tctgaacagt tatatactgt gtatatacgt ggtaatgacg aggtggcaaa
      241 tggcacaggt taatatgagt gtaagaatcg acgcagaact gaaggatgct tttatggccg
      301 ctgcaaaaag catggaccgt aatggctctc agttaatccg ggattttatg cgccagaccg
      361 ttgaacggca gcacaattct tggttccgtg actaggttgc ggcaggccgt cagcaactcg
      421 aatgcggtga tgtgcttccc catgacatgg tcgaatcctc tgctgctgca tggcgggatg
      481 aaatgagcag gaagattgct gataaatgat ggagatgttc tggaccatgc tggccatcca
      541 ggacaggaag cgcattcgta gtacattgcg gagcagaacc tgatggccgc cattgaaccg
      601 gacgaacgga ttggttattc agccagcagc ctggcagggc agccgtataa aggccgcaac
      661 ggtcgtgtgg aaggaacatc tggaccacac aaggtagcgt gtaacgttat tttgtgtgaa
      721 aatctcttgt gatttttaaa tatcgtaaca aaggtatgac atgaaaataa catctaccat
      781 tattcaaaca ccttttccat ttgagaataa taattctcat gctggcatag taacggagcc
      841 cattctcggt aagttaatag gtcaggggtc gacagcagaa atctttgaag atgtgaatga
      901 ttcatctgct ttgtataaaa agtatgatct tattggcaac cagtacaatg agattctgga
      961 aatggcttgg caagaatctg agctttttaa tgctttttat ggcgatgaag catccgttgt
     1021 tatacagtat ggcggagatg tgtacctccg aatgctgcgc gtgcctggga ctccccttag
     1081 tgacattgat acagctgata tccctgataa tatagagagc ctttatctac agttgatatg
     1141 taaattgaat gagttgagta taatccatta cgatcttaat acaggtaata tgctgtatga
     1201 taaagaaagt gaaagtttat tcccaataga ttttcgcaat atttatgctg aatattacgc
     1261 tgcaaccaaa aaagataaag agattatcga ccgacgatta caaatgcgta caaatgattt
     1321 ttattcgtta ttaaacagga aatatttata gacgtatttg ttgatgctat aagccagagg
     1381 ctcttctatg agaatatggg gaccattccc tcgcgatatt aacgtaatat cttaattgta
     1441 tgcaccgcca gaatgcgttc agtgcgatca cggcagcaga caggtaaaaa tagcaacaaa
     1501 ccacccgaaa aaccgccgcg atcgcgtatg ataaatttta accacatgca tacctgtgca
     1561 acaaggcgaa tcacgcacgg atttccagcc agagacacct gaaacgtaac cggctcattt
     1621 aaaccgtctg gtctgttacc tccggttcca caaaaataat gtccatcatt ttgaagtgga
     1681 cactatcgta tggaataccg gacctgaatc actgaagctt tacgccttca tttcgaagaa
     1741 catttaccac gggttgtggc cggatgtcgc ctgggcgtac caaaatcaac tgtctgtggt
     1801 atgttcgtgc gcttccgcaa cgctggcctt tcatggcctc tgcccgcagg tatgtcggag
     1861 caggaacttg atgcccttct ttacggaagt gcctccacag tacctgtcgt acttactgaa
     1921 agcaccgtta tgcccaaact cccggtggta aaaaaacgtc cccggcggc
//
LOCUS       DAEBXN010000301         1964 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.306, whole genome shotgun sequence.
ACCESSION   DAEBXN010000301 DAEBXN010000000
VERSION     DAEBXN010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.306"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(114..1568)
                     /gene="ospC2"
                     /locus_tag="KJL99_004530"
     CDS             complement(114..1568)
                     /gene="ospC2"
                     /locus_tag="KJL99_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HBD4438972.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKINPNKDTKALQKNISCVTNSSS
                     SGISEKHLDHCADTVKSFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYLSEIIQKTHPLSSDERHLLSIIINSDFNFRHQSNANLSNNTLNIKSFD
                     KIKSENIQTYKNTFSEDIEEIANHDFVFFGVEISNHQETLPLNKTHHTVDFGANAYII
                     DHDSPYGYMTLTDHFDNAIPPVFYHEHQSFFLDNFKEVVDEVSRYVHGNQGKTDVPIF
                     NTKDMRLGIGLHLIDFIRKSKDQRFREFCYNKNIDPVSLDRIINFVFQLEYHIPRMLS
                     TDNFKKIRLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSLGNAKSDMALYLLSK
                     FNFTKQDIAEMEKMNNNMYCELYDVEYLLSEDSANYKVLEYFISNGLVDVNKRFQKAN
                     SGDTMLDNAMKSKDSKTIDFLLKNGAVSGKRFGR"
BASE COUNT          622 a          332 c          311 g          699 t
ORIGIN      
        1 tcaccacatg caatgtgttg ttgcagggga acggtctgca ccatccccta tatcgatcaa
       61 tataacaaaa atgacaacgt cagattcagg gcacaaccta aatatatttt aggtcacctc
      121 ccaaatcgtt tgcctgatac cgctccattt tttaataaaa aatcaattgt ttttgaatct
      181 ttgcttttca ttgcattgtc aagcatagtg tccccactat ttgctttttg aaatcttttg
      241 tttacatcaa ccaatccatt actaataaaa tattctagca ctttatagtt ggcactatct
      301 tcactaagta aatactcaac atcatacagc tcacaataca tattgttgtt cattttctcc
      361 atctctgcga tatcttgttt tgtaaaatta aatttagata gtaaataaag tgccatatca
      421 gattttgcat tgcctagaga ataagcaaga gcttgatgag ccattttttt atcagtcacc
      481 ttattgttaa tttcttcata attggatgct ttaatggcat cctccaaaga tatatctctg
      541 agtcttatct ttttgaagtt atctgtgctt agcattcttg gtatatgata ctcaagctga
      601 aacacaaagt ttataattct atccagacta acaggatcaa tgtttttatt ataacaaaac
      661 tctcggaatc tttgatcttt actctttctt ataaaatcaa taagatgcaa cccaattcca
      721 agcctcatat ctttagtatt aaatattggc acatcagttt ttccctgatt accatgcaca
      781 tacctgctaa cttcatccac aacctcttta aagttatcta agaaaaatga ttggtgctca
      841 tggtaaaaaa caggtggaat agcattatca aagtggtcgg ttaatgtcat atatccatat
      901 ggagagtcat gatcaatgat atacgcatta gcaccaaaat ccacagtatg atgtgtttta
      961 tttagtggga gtgtttcttg atgattggaa atttcaaccc caaaaaaaac aaaatcgtgg
     1021 ttagctattt cttcaatatc ctcagagaat gtgtttttat atgtttgtat attctcagac
     1081 ttaattttat caaaggactt aatattaaga gtattatttg acaggttagc gttactttga
     1141 tgtctgaagt taaaatcaga attaataata atagagagca agtgacgttc atccgaagaa
     1201 agaggatgag ttttttgtat tatttcagaa agatagtgtc tggacacctt atcgaggtgc
     1261 tcaagtgacc gaaatgatga ccgtgctcca gatggcgctt caatagaaag atttaatgat
     1321 ttgaaactgg tcccttgaga gaacatttta ctataacttt gcgctgctat agacttcctt
     1381 aaaaagcttt tcacagtatc agcacagtga tctaaatgtt tttcacttat gccagaagag
     1441 gatgagtttg ttacgcatga tatgtttttt tgtaatgctt ttgtatcttt gttcggattt
     1501 atttttccat caacaagatc aatattgttc tgaacattaa tatgatttac tgcttcaggt
     1561 attttcataa aacatccccc attatgtgcg tttttatctg cagttattta gaataatatt
     1621 atctaactta atattttctt gtatgtatta taacgaacat attttaatat gttgtacgat
     1681 tttcactctg aaatcccctg agtttaatca cacttccttt actgaaagga attattgcaa
     1741 cagtattatt tgtttttgga tatatatctt gcgccttgtt atgtagttaa tgctcagaat
     1801 atgatagata aaatagagac gcaaaacagg gcatgctcat aacatgatga taacataaaa
     1861 aattagtaac aataaatgaa agaatcatgg gcgctcaaca aaaagagggt aatgctgcca
     1921 acttactgat ttagtgtatg atggtgattt taaggtgctt gcgt
//
LOCUS       DAEBXN010000302         1919 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.307, whole genome shotgun sequence.
ACCESSION   DAEBXN010000302 DAEBXN010000000
VERSION     DAEBXN010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.307"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(59..1708)
                     /locus_tag="KJL99_004531"
     CDS             complement(59..1708)
                     /locus_tag="KJL99_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HBD4438973.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT          452 a          510 c          491 g          466 t
ORIGIN      
        1 cctcctgaag aggataattt tacagccagc aggaagttag cggctggctg taaaaatatt
       61 atggttcgcc gtacaaacca attaaacggc gtaccacacc gttagtccag ccaaacccat
      121 cctgcaacgg atactcgcca ccgccgcctt cgcggggaac accatcggca atatggtatt
      181 tctcgatcat tttgtgctgt tccagataga actgattcac cgttttcagc cagctgcgcg
      241 cgatttcatc acccagaagg tcatcgccat acattttaaa tccctgaatt gccatccatt
      301 gtaacggtgc ccagccgttg ggtttatccc actgttcacc ggtttcgtac tcgcttgcca
      361 aaatcccgcc aggtgtcagt aaccggctgc gtacggcgtt tgccagacga tcggcctgtt
      421 catggttcgc catgccgaca tagagcggca caatggcggc agcggaaaac agcgccagtt
      481 gttcgcgtcg ccagtcgtaa tcgcggtaga tgccgttttc atcatcccag aggtaacggt
      541 ttaccgcatc gcgacgggca ctggccttct ggcggaacag cgcttctgtc tctttctcgc
      601 ctttcagcgc cgagatgttg gcgatggcgc tctccagttt aaacaggaag gcattcagat
      661 cgatggggat gaactgggtg gtacgaatgc tcgccagacg accagtatca cgcagccaac
      721 gggaagagta atcccaaccg gaggccgctc ccgcgcgtaa atcgcggtac acctcgttgg
      781 gcgggcgacc agaatgtttc gcggtttcaa cgtcctcaag ccaggattcg tcacgcggcg
      841 tgtcgcgatc gtcccagtaa cggttgagca gcgatccgtc cggcatccgc acaacatggc
      901 gataggcctg attagggatc aacgattctg caccgtccat ccagaaggca tattccattt
      961 taaggtggtc gagatagcgg cgcgcaccgc gtacaccatc ttcttcaaac aactccacca
     1021 tcagcgcaaa aaccggtggt tgcgagcggc tcaaataata ggtgcggttg ccgttgggga
     1081 tgtgaccgta gttttcgatc atccaggcga agttatcggc catgcatttc agcaaatctt
     1141 cccgaccact ttccgccagt cccagcatgg tgaaatagga atcccaatag taggtttcgc
     1201 taaaacggcc gcccgggaca atatatgact gcggtagcgc cagcagagaa gaccacggaa
     1261 tgtgatcctg tggttcgcgg gttagcaccg gccacagctg gtcgatatgc tctttcaggg
     1321 aattttgcgg gtccgataca tactcgctgg agtagacctc cggcagccag aagtgatttt
     1381 caacaaactt gcgcaagtca aaatcacgat gacggcgcac tttacggtag cggattaaaa
     1441 tatccagcgg gtccattttg ggtgcgcagt cgggaaaggt tttactgtcg gggaaaattt
     1501 tcgccgactg aacatgctcg aacagttcga gatagcgatc ggcaggcgtc agcgcatcag
     1561 aggcgggcag cccttcaatc atctcgggtt ccggttctgc ctcgatcatc tcatccagtt
     1621 ttaattcata cgggtccagt tcatagcaga gatcgacctc gatcatcagt tcgtctggat
     1681 tagggttttg aattttctga ttgagcataa ggatccaacc tccgaaattc gtcgctaatt
     1741 atcgggcttt tctttaaggg tagtcattcg ctccgcctgg tgggcgacaa agcgtgcggt
     1801 ggatcacgtt tattaacttg tcaaaaaata taacgcacta atatcaatgg aatttataaa
     1861 aatacggatt tggtgttgtt tttcattttc agaacattca atcctgctct cgccgcaat
//
LOCUS       DAEBXN010000303         1917 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.308, whole genome shotgun sequence.
ACCESSION   DAEBXN010000303 DAEBXN010000000
VERSION     DAEBXN010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.308"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(153..227)
                     /locus_tag="KJL99_004532"
     tRNA            complement(153..227)
                     /locus_tag="KJL99_004532"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(192..194),aa:Gly,seq:tcc)
     gene            complement(231..306)
                     /locus_tag="KJL99_004533"
     tRNA            complement(231..306)
                     /locus_tag="KJL99_004533"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(271..273),aa:Thr,seq:tgt)
     gene            complement(309..385)
                     /locus_tag="KJL99_004534"
     tRNA            complement(309..385)
                     /locus_tag="KJL99_004534"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(349..351),aa:Arg,seq:tct)
     gene            complement(387..462)
                     /locus_tag="KJL99_004535"
     tRNA            complement(387..462)
                     /locus_tag="KJL99_004535"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(427..429),aa:Ile,seq:cat)
     gene            complement(639..1328)
                     /locus_tag="KJL99_004536"
     CDS             complement(639..1328)
                     /locus_tag="KJL99_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HBD4438974.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGASIPLVSQVAFSTSSWRRAVLSLEEHYK
                     AWLLWCYSGSICWEYQITITHWAWEEFKAHSGTRKIAEKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTGHWVVMKHIFHRLDSEALLFVMRTRSEQKA
                     AFSKQSIAKVD"
     gene            complement(1325..1684)
                     /locus_tag="KJL99_004537"
     CDS             complement(1325..1684)
                     /locus_tag="KJL99_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBD4438975.1"
                     /translation="MRHEFILPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDETNIVRGL
                     PVPGGRLGIKITELECA"
     gene            complement(1684..1821)
                     /locus_tag="KJL99_004538"
     CDS             complement(1684..1821)
                     /locus_tag="KJL99_004538"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438976.1"
                     /translation="MVPALRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEVL
                     R"
BASE COUNT          488 a          529 c          411 g          489 t
ORIGIN      
        1 gtaacctgac cgacaatgca ccattctggt gctgaaaaaa gagcactgga actgcaacaa
       61 aacaacatca cagaacagga gatggtaagg agtcagacat tcccacacaa tattgtgtca
      121 gtgcaaataa caaccttcgt ctcaaatctc gctggagcgg gcagcgggaa tcgaacccgc
      181 atcatcagct tggaaggctg aggtaatagc cattatacga tgcccgcata tggtgccgac
      241 taccggaatc gaactggtga cctactgatt acaagtcagt tgctctgcct gctgagctaa
      301 gtcggcgctg gcccaccacc gaggactcga acctcggacc gtcaacttag aaggttgatg
      361 ctctatccgg atgagctagt ggtgtttggt ggcccttgct ggacttgaac cagcgacctg
      421 gcgattatga gtcgctcgct ctcaccactg agctaaaggg ccggaggcag aataataacc
      481 atatgtcatc acatctgcaa actcatctga ccaccagcac gtttaacgtc ctgtgccatt
      541 tttcaggcat aaaaaaaccg ctcggcggcg ggttttgctt actttaccat cgcgtacaaa
      601 atcggcaaag tatcagattt gcatgagata tatgcctttt aatctacttt tgcaatactt
      661 tgctttgaaa atgccgcttt ttgttctgaa cgtgttctca tcacaaacaa taaagcctca
      721 ctatccagtc ggtgaaaaat gtgtttcatt acaacccagt gaccagtaaa tgttttggac
      781 cagtttttgg ttgttactcc cgccagtaat gccagctcct gatattcata accttcccca
      841 ccaaaaagtt ctgcttttac cgcctgcgcc gccagccaga ttaatttttt caggcgttcc
      901 tgtgttttct ctgcaatttt cctagtgcca gaatgagcct taaactcttc ccacgcccag
      961 tgggttatgg tgatctgata ctcccagcaa atacttccgc tgtaacacca caacaaccag
     1021 gctttatagt gctcttcaag agacagaaca gcccgccgcc acgatgatgt cgaaaacgca
     1081 acctgactga ccagaggaat tgacgccccc ttcgccagtg attgttttcc cgggattggt
     1141 ggattatccc gcgttatcat ttttccggtc acctcatcgc ggtaccggat ttttttacgc
     1201 ctgtaacgcc ctgtatcgaa catggcattc tcctgccagg cttcaagctg accttttgtc
     1261 gccccactca aatcagcggt ggcgataatg agctgctcac gaacaaactg taaatactgg
     1321 ttattcatgc acactccagc tctgtgattt ttatccccaa ccgaccacca ggaacaggca
     1381 gtccgcgcac aatattggtt tcatcaaact gctcgtcgtc gataagcaac cccgcatgtg
     1441 tcagtgcatc cagtggtgct ttcaggatat tgtccaggtc acgacgccgc ttatccggtg
     1501 gctctgcaat aatctttatc gccagtcttc cggacaggct taatttcagt cgctgctgac
     1561 ggacaataag cgccacatcc cggcgataac gctcaccggc ttttgataca aaatatgtgc
     1621 tgccacgacg tcgccagtaa gtattcaccg tcggcggata aggtaaaata aactcatggc
     1681 gcatcagcgc agcacctcct gcaccagctt ttcaaacttt ccgactctgg tttccagctc
     1741 tgccacacag tccaccagct catctactgc tttctgtgcg cgatgcttcg cctgcatcag
     1801 ttcccgaagc gcgggtacca tattgaggta gcctgagttt aacggacact ccttcctgaa
     1861 atagaatggc atcagaagga gccaataatg agcagaaaaa cccaacgtta ctctaaa
//
LOCUS       DAEBXN010000304         1890 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.309, whole genome shotgun sequence.
ACCESSION   DAEBXN010000304 DAEBXN010000000
VERSION     DAEBXN010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.309"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(<1..362)
                     /locus_tag="KJL99_004539"
     CDS             complement(<1..362)
                     /locus_tag="KJL99_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD4438977.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGL"
     gene            complement(429..1643)
                     /locus_tag="KJL99_004540"
     CDS             complement(429..1643)
                     /locus_tag="KJL99_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HBD4438978.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIGYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPYSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPEYLP
                     VARLEDGTLLNW"
BASE COUNT          578 a          481 c          382 g          449 t
ORIGIN      
        1 agaccgatac gcattaaaat aaagtggcta aaagaattaa ccattcctgt taatgcctgc
       61 attaatgacc agaatattaa gcctgcgcct aaaactattc gtgggccaaa gcggtctagc
      121 aaaataccgg aaggtagttg tgaaaaacca taagacagag aaaatgcaga aagcaaaaca
      181 ccaaactctg tcgccgataa tcctaactcg ccacgaatcg cttcgcctgc cacgctcaat
      241 gacgaacggt cgagaaaatt aacgatcccc gccataaata ataaaaccag ggtcactgtc
      301 tgaatacgac gaatccgttg cggtttatta cccgcaacac tggttgacac atcaacgtcc
      361 atttttagct ccttgagcgg aattaatcga tttgatgact attcagaaaa taattaagcg
      421 aaccgttatt accagttcaa caatgtgcca tcttccagtc gtgcgacggg gagatactcc
      481 gggtcatacg gatatttcgc cgccagtttc tcatcgaaag aaatacccag ccccggttca
      541 tcgcctgggt gcatataacc ttcctcgaag cgccagctgt acgggaaaac ttcaagcatt
      601 tgttcggaat agcccatgta ctcctgaacg ccaaaatttg gtacccatag gtcaaaatgc
      661 agcgccgccg catgacaaac aggcgataaa tctgaagggc catgcgaacc ggtacgtacc
      721 tgataaagcg atgcaaaatc ggcaatccgc cgcatcccgg taatgccgcc tgcatgggtg
      781 atcgtcgtgc gaatatagcc aatcagctgc tcttcgatta actgcttgca gtcccagata
      841 ctgttaaaca cttcaccgac cgcaatcggc gtcacggtat gctggcgaat cagacgaaaa
      901 cattcctggt tctcggcagg cgtcggatct tccatccaga acaggcggta atcctcaatg
      961 ctcttcccaa agcgcgccgc ttcgatgggg gtcaggcgat ggtgcatgtc atgcaacaga
     1021 tgttcgttaa aaccgaactt attacgcacc gcttcgaata acttcggcgt aaaatcgaga
     1081 tacttttctg aagaccacaa ctgctcttct ggccacaggc ctttggttgc cggttcatac
     1141 gccagtcctt tgcccttcgc caggccataa gtggtttgca tacccggtac accgcactgc
     1201 acacgaatcg ccttaaagcc catctgctgg tgctttgcat agtcttcaag gacttcatca
     1261 atcgtacgcc cggtggtgtg gcaataaacc atcacgcctt cacgagaagc accaccaagc
     1321 agttgataca atggcatatt tgccgcttta gctttgatat cccacaacgc catatcaatg
     1381 gcagaaatgg ctgacatcgt gacggggcca cgtcgccagt aagcaccttt ataaaagaat
     1441 tgccagatat cttcgatacg gctggcatcg cggccaatta gttgtggaca aagatgatct
     1501 ttcagataag aagcgaccga caattcgcga ccatttaacg tggcatctcc taatccacaa
     1561 agtccgcttt cagtggttat tttaagcgta acgaaattac gccctgggca agtaacaaaa
     1621 acatctgcag caataatttt cattattgca atctcctgtg ttgtaaatag acgtttcaaa
     1681 cctaatttta cgcctactac catacaagag agaaaaagcg attattcaac cgtgatcaca
     1741 ttaaaaacaa aaatttctac cgaggagaaa aaataccctc aaaaaccacc tgaataatta
     1801 accattattg ttaaaacaac acgttgaata atcacataac aaactccatt aatcattgaa
     1861 aatgagcaca gaaatagaaa ttaattatat
//
LOCUS       DAEBXN010000305         1871 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.310, whole genome shotgun sequence.
ACCESSION   DAEBXN010000305 DAEBXN010000000
VERSION     DAEBXN010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.310"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(1..355)
                     /locus_tag="KJL99_004541"
                     /pseudo
     CDS             complement(1..355)
                     /locus_tag="KJL99_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(355..1275)
                     /locus_tag="KJL99_004542"
     CDS             complement(355..1275)
                     /locus_tag="KJL99_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBD4438979.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPHIEAGSNARTSDISSEEYAIMPLAPMKEADAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1413..>1871
                     /gene="pgrR"
                     /locus_tag="KJL99_004543"
     CDS             1413..>1871
                     /gene="pgrR"
                     /locus_tag="KJL99_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HBD4438980.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMVQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPILHDIDSALTSLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVLRCTGNSG"
BASE COUNT          467 a          458 c          435 g          511 t
ORIGIN      
        1 ggcacccccc acacacaccc agcacgccaa tgcgttcagc atcaacatat ggcagagtta
       61 ctgcataatc gataacacgg ctaatatcct ctacgcgctg tgtcggatct tctatccaac
      121 gaggctaccc accagattct ccctggaaac tggcgtcata agcgagaacg acatagcctt
      181 tttcagccaa tgccttgcca tagatattgc cagacgtctg ctccttacaa ctgccaaaag
      241 gatggacgct gataatcgcc ggatactgtt ttgactcatc aaaatttggt ggaaactgaa
      301 tattggcagc aatatgccag gccatatcac tattgcggat ttgaatatgt ttcattacag
      361 agtttcctca aagaacggag ctaatactga aattgcttca gccacatagg ctttgccgtc
      421 ataaagatcc atatggtttg cgccttcaac aatgtgatag cgtttatcct ggcttgatgc
      481 ccgatcgtac aggtcgtcac tcatccattt gctccctgcc tggctgcccg ccacaatctg
      541 catcggctga gtcaggtaca cttccgccat atggtaagca tcataggtaa taatctggtt
      601 aaggctgcgc aaagtagcgt aacccggtgc tgttggatac tgcgcacgag gtgtgtggta
      661 atactcccag gcctgacgca gttcttcgtt cggcgcatcg gcctccttca ttggagccag
      721 tggcataatg gcgtattctt cgctgctaat atcgctggtt cgggcgtttg agccagcttc
      781 aatgtgtggc agagcatcaa tagatttcac attgttttcc caaccattgc ggaacatcga
      841 accaatattg accgcactaa cggtaccgat ggccttgatg cggcgatcct gaattgcagc
      901 attggctgta tatcctgcac cggcacaaat tcccatcgca ccaattcggg tattgtcgac
      961 ataagaaagc gttgtcaggt aatcaatcac ggcactgacg tcttcagtac gaatgtatgg
     1021 gttttctaac tgacgcggct cgccgccact ttcaccttga taagatgcgt cataagcaat
     1081 agtgacaaat cccttttccg ccagcttttc ggcataggtt ccggccgttt gctctttaac
     1141 gcccccacct gggtgagata ccacaattgc cggatactga cgggtttcat caaattttgg
     1201 agggaaatag atcactgcag acaaagagat ggttggatta ttgctgttag tgaagctgac
     1261 tttattattc atcattcagt tcctcatgga gttgtcggtt cgttttgacg gttggtgata
     1321 tcactataga tattgatcat taagttgatt agaccctaaa tcatgattag acctatcatt
     1381 taaatgatta atagattgta tggagatact gaatgaagcg tgaagaaatc gctgatctga
     1441 tggcgtttgt cgtcgttgca gaggagcgta gcttcactcg cgcagcagcc cgcctgagca
     1501 tggtgcagtc agctttaagc cagatagtgc gccgtataga agaacgattg ggattgcggc
     1561 ttctgacgcg aaccacgcgc agcgttgtac ccactgaagc gggcgagcat cttttgtctg
     1621 ttcttggccc tatattgcat gacatagatt ctgccctgac atccctgagc gatctgcaga
     1681 accgcccatc cgggacaata cgtattacta ctgtagaaca tgcagcaaaa acgatattgt
     1741 taccagccat gcgcacattc ctgaaatcgc atcctgaaat tgatattcag cttaccattg
     1801 attatggttt gaccgatgtc gtttctgaac gttttgatgc aggcgtcctg aggtgtactg
     1861 gcaatagcgg a
//
LOCUS       DAEBXN010000306         1849 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.311, whole genome shotgun sequence.
ACCESSION   DAEBXN010000306 DAEBXN010000000
VERSION     DAEBXN010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.311"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(<1..257)
                     /locus_tag="KJL99_004544"
     CDS             complement(<1..257)
                     /locus_tag="KJL99_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438981.1"
                     /translation="MSLQVSHYNMLRASHEGSQKVVVRTVITVRFVPGAAIAKSILYC
                     AGQLFFKESGHHLTGTDTIYFVTVRLHMHNSTYSSIFCFSMS"
     gene            282..521
                     /gene="relB"
                     /locus_tag="KJL99_004545"
     CDS             282..521
                     /gene="relB"
                     /locus_tag="KJL99_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HBD4438982.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            521..808
                     /gene="relE"
                     /locus_tag="KJL99_004546"
     CDS             521..808
                     /gene="relE"
                     /locus_tag="KJL99_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HBD4438983.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
     gene            880..1035
                     /gene="hokD"
                     /locus_tag="KJL99_004547"
     CDS             880..1035
                     /gene="hokD"
                     /locus_tag="KJL99_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HBD4438984.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1253..1504
                     /locus_tag="KJL99_004548"
     CDS             1253..1504
                     /locus_tag="KJL99_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438985.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNIVSHYRIVCLQKNGAALIN
                     FVDARVTDGFILRDGEFVTSLQALKEIGIKAGFSAFSEE"
     gene            1571..1849
                     /locus_tag="KJL99_004549"
                     /pseudo
     CDS             1571..1849
                     /locus_tag="KJL99_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          507 a          410 c          468 g          464 t
ORIGIN      
        1 gacatgctaa agcaaaaaat agatgaataa gttgagttgt gcatatgtag cctgaccgtc
       61 acaaagtata tggtgtctgt accagtaaga tgatggccgg actctttaaa aaagagctga
      121 cctgcacaat acaggatgga cttagcaatg gctgctcctg gcacaaagcg gacagtgatc
      181 accgttctta cgactacttt ctgacttcct tcgtgacttg ccctaagcat gttgtagtgc
      241 gatacttgta atgacatttg taattacaag aggtgtaaga catgggtagc attaacctgc
      301 gtattgacga tgaacttaaa gcgcgttctt acgccgcgct tgaaaaaatg ggtgtaactc
      361 cttctgaagc gcttcgtctc atgctcgagt atatcgctga caatgaacgc ttgccgttca
      421 aacagacact cctgagtgat gaagatgctg aacttgtgga gatagtgaaa gaacggcttc
      481 gtaatcctaa gccagtacgt gtgacgctgg atgaactctg atggcgtatt ttctggattt
      541 tgacgagcgg gcactaaagg aatggcgaaa gctgggctcg acggtacgtg aacagttgaa
      601 aaagaagctg gttgaagtac ttgagtcacc ccggattgaa gcaaacaagc tccgtggtat
      661 gcctgattgt tacaagatta agctccggtc ttcaggctat cgccttgtat accaggttat
      721 agacgagaaa gttgtcgttt tcgtgatttc tgttgggaaa agagaacgct cggaagtata
      781 tagcgaggcg gtcaaacgca ttctctgaac caaagcatga catctctgtt tcgcaccgaa
      841 ggtgacactt ctgctttgcg ttgacaggag aagcaggcta tgaagcagca aaaggcgatg
      901 ttaatcgccc tgatcgtcat ctgtttaacc gtcatagtga cggcactggt aacgaggaaa
      961 gacctctgcg aggtacgaat ccgaaccggc cagacggagg tcgctgtctt cacagcttac
     1021 gaacctgagg agtaagagac ccggcgagtg agaaatccct cgccacctct gatgtggcag
     1081 gcatcctcaa cgcacccgca cttaacccgc ttcggcgggt ttttgttttt attttcaacg
     1141 catttgaagt tctggatggt gccggaatag aatcaaaaat acttaagtag cgcgcaggga
     1201 taaaagggat ggaccccgaa caggggagtg ctacttatct ggaaggattc tgatgatgaa
     1261 catcgaagaa ctgcgtaaaa ttttttgtga agatggcctc tatgctgtgt gcgttgaaaa
     1321 tggaaatatt gttagtcatt accgcattgt gtgtttgcaa aaaaatgggg ctgcgttaat
     1381 taattttgtg gatgcccgag tgacggacgg atttatcttg cgcgacggtg agtttgtcac
     1441 ttcattacag gcattgaaag agatcggaat aaaagctggc ttttctgctt tttcagaaga
     1501 ataaactcat ctacaatctt gcgcggggct gaactcccgc tgagtaacac cgtgccaccg
     1561 gagaaaaccg atggcacgca acgcaaaata ttacaatcat gataattcga ccgttcttgc
     1621 ccacacgcac gagcggtatt ctcacgcatt taagtcagac tggtaccagc atccctcatg
     1681 cactgaagaa caggccgaat ggcttattca gtgttaccgc aggcgcggat gcgaggttaa
     1741 aaaagccctt agcctcgact accgtcacta gataatctcc gtcaggctcc cttactccga
     1801 acagccaccg cgtccgtccc gcacattcca gcaacggatc tggaggtaa
//
LOCUS       DAEBXN010000307         1833 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.312, whole genome shotgun sequence.
ACCESSION   DAEBXN010000307 DAEBXN010000000
VERSION     DAEBXN010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.312"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(<1..181)
                     /locus_tag="KJL99_004550"
     CDS             complement(<1..181)
                     /locus_tag="KJL99_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HBD4438986.1"
                     /translation="MAGCVGEPKGSPVSVPGYANPAQFATSSIGVVGGDNFQMYGVIV
                     MTTQISVETLSPITHN"
     gene            complement(322..876)
                     /locus_tag="KJL99_004551"
     CDS             complement(322..876)
                     /locus_tag="KJL99_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HBD4438987.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNQLLASKKLTKTAVNEALRRMKKAGIEKPELEAFLREMINGKQKTWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPEWCFRTCCSRVDVWL
                     NLAEYMLYLPMRDAFSSGDLKTVC"
     gene            complement(873..1163)
                     /locus_tag="KJL99_004552"
     CDS             complement(873..1163)
                     /locus_tag="KJL99_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HBD4438988.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHNPETSVLAHYRLAGTCGTATK
                     PHDMQVAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(1163..1762)
                     /locus_tag="KJL99_004553"
     CDS             complement(1163..1762)
                     /locus_tag="KJL99_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HBD4438989.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSDAAEQ
                     YLNTVATCRTRDTALLKSFDAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEEVENVAAQLLEFA"
BASE COUNT          446 a          496 c          421 g          470 t
ORIGIN      
        1 ggttatgggt gatcggggag agagtttcaa cagagatttg agtggtcata acgataactc
       61 cgtacatttg gaaattatcg ccaccgacga cgccaatcga actggtggcg aactgtgcag
      121 ggttggcgta accgggtacg gaaaccggcg agcctttcgg ctcccccaca cagcccgcca
      181 taaatcgcga atgtgactgt gcaaacgata tgaaaaaaga cgcgggcgcg tctcatatcg
      241 ctccgtaaac atccgggacg ccaatcccga cgccagattt tgctggcgcg tgaggaatat
      301 agccccggat aacagattga gtcaacaaac ggtttttaga tccccggaag agaatgcatc
      361 acgcatcggt agatagagca tatattcagc aagatttagc catacatcta cccgactgca
      421 gcacgtccgg aaacaccact cagggtgtgc gtcattcagc aattcagcca ttttacgctt
      481 agtcatcccc cgcccttcat accgttgccg gaggacacta atcaatccag gatgctctgc
      541 cagcacctca cttatgactc tatcaataca taacgcctct gcatcagtac aatgcgccag
      601 ccaggtcttt tgcttgccat tgatcatctc tcgcaaaaac gcttccagct caggtttctc
      661 tattcccgct tttttcatcc tgcgcagggc ttcattgacg gctgttttcg tcaatttttt
      721 ggatgccagc aactggttaa acatatttcc cgtcttaccg ccgccaatat acgaccagcg
      781 cccccacatg cgcagttttc cctgaatcca gacactttcc agcgtggtga gacgaaggtg
      841 ttccccgctt ttgcctgtat ttgttgggta aatcataaat aaccttcctt tctccagatt
      901 tcttgtgtgc gaaaaacacc ttctgcatgc atcaggcgta attcttcttt ggtgtaatcg
      961 ctggttttta cccgcccgtc gattaaatcg tggcatgagc tacaggcaat cgctacctgc
     1021 atatcgtgtg gttttgtcgc tgttccgcac gtccccgcca gcctgtaatg cgccagcaca
     1081 gaagtttcgg gattgtgatt gcagtagcca ggaattctga cggtgcacat ctgcccccgc
     1141 gccgctttac gtaaatccac cattacgcaa actccagtaa ctgcgcggcc acattttcga
     1201 cttcctccgg agaggaaaat ttacggaaca ggatccagtt ccacagcaca ttcagtacag
     1261 atttataaac ctgctgaaac tcggtttcgt ccatgttcgc aaatgcgata gattttgccc
     1321 tgcgcccacg actaccatca ggataaatat gctcggtgta aaatccggcc tgaatggtta
     1381 cccactcgcg gaaagcgtca aacgacttta gcaatgccgt atcccgggtt ctgcatgtcg
     1441 caactgtatt aaggtattgc tctgcggcat cactcagggc tggagtgtgt tcccgaccta
     1501 ctgattcgca caggtaatca acgaagcctg ataccagttt tcgttcgcga ggcgtgatcg
     1561 ccccaccgac cggagtccag taatcaaatc ccagttgcag gagtttgaaa aaacgcttgt
     1621 ggaatgcgta gttacgcaca cgcttaaagt ctgcgtgtat ccactcacct attttgattt
     1681 gatgcaaaaa atcgcaactc tccggcgtcg ccgggagaag taatccggaa gaggtttgtt
     1741 tgaccagttg tatatgcgcc atcgtagttc tccgctggcg cagtagaatg ggtgttcagc
     1801 ccgttatgta gtataccaga attaatgcca ata
//
LOCUS       DAEBXN010000308         1826 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.313, whole genome shotgun sequence.
ACCESSION   DAEBXN010000308 DAEBXN010000000
VERSION     DAEBXN010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.313"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(208..609)
                     /locus_tag="KJL99_004554"
                     /pseudo
     CDS             complement(208..609)
                     /locus_tag="KJL99_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            1038..1826
                     /gene="virF"
                     /locus_tag="KJL99_004555"
     CDS             1038..1826
                     /gene="virF"
                     /locus_tag="KJL99_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HBD4438990.1"
                     /translation="MMDMGHKNKIDIKVRLHNYIILYAKRCSMTVSSGNETLTIDEGQ
                     IAFIERNIQINVSIKKSDSINPFEIISLDRNLLLSIIRIMEPIYSFQHSYSEEKRGLN
                     KKIFLLSEEEVSIDLFKSIKEMPFGKRKIYSLACLLSAVSDEEALYTSISIASSLSFS
                     DQIRKIVEKNIEKRWRLSDISNNLNLSEIAVRKRLESEKLTFQQILLDIRMHHAAKLL
                     LNSQSYINDVSRLIGISSPSYFIRKFNEYYGITPKKFYLYHKKF"
BASE COUNT          570 a          330 c          340 g          586 t
ORIGIN      
        1 caagggaata tagtttgtgt aagcatattg cttccttatg ttatatttta gtaaagaatt
       61 tattttttac attatagtcg gctaaatgaa gtttactgta aaaaaacgat acttttttcg
      121 taagcgatga gatgtggagc cagaaaaatg gctgcgttac gtcattgaac tgtccttgac
      181 ccacgatatt cagacagtcg ttgtatctta agttgacatt acccgctgac gtcgatattc
      241 ccttggtgag agataaccca acgcactatg cggatggttt tcattgtaat gctcgatcgc
      301 cactgcaagg ttatgcaatg ccgttcttgg tttcggcatg aacgcgatgc agtcttcctt
      361 catcgttttc atgaaccgtt ctgctattcc attactctga gggctactga ctgccgtcgt
      421 gcagggctcc agattcaact ctctggcgaa ctgccgtgtt tcatgagccg tataagccga
      481 accgttatcg gccactggat tggccgttcc ggtcctttat cgccatatcg ctcctgacat
      541 gacatcctgc accgttgctt tgtcataatc tcccgtgcct gctgcccagt ctatggcttc
      601 acgatcgcaa tatggaacgt aacgctgtca tcgggagaat cgtcaatgcg gcattcggtt
      661 aacgcttacc aatatcaaca ccaaccggat ttgtggagcc tccagtctga aggggcttta
      721 tgcgttccgt ataggatatt atgatgctgg agttttgcga agctccactc ctttccgaac
      781 atttacacag atattgccta agaaaagtga aaccataata tggttatagt ccctttcagt
      841 gcaaatactt agcttgttgc acagagaaat agaagctgca taagctcttt cttcaaaaaa
      901 tgtaaataaa gttaaatata ggaaaaatta cttaatctat cttaataacg gaaagatttt
      961 gtatacaatc actgttacag aaatttctta gttactctgt aaacactaaa tatagtttgg
     1021 tatattctgt tgaatttatg atggatatgg gacataaaaa caaaatagat ataaaggttc
     1081 gcttgcataa ctatattatt ttatatgcaa aaaggtgttc aatgacggtt agctcaggca
     1141 atgaaacttt gactatcgat gaagggcaaa ttgcttttat agagcgaaat atacaaataa
     1201 acgtctccat aaaaaaatct gatagcatta atccatttga gattataagc cttgacagaa
     1261 atttattatt aagcattatt agaataatgg aaccaattta ttcatttcaa cactcctatt
     1321 ctgaggagaa aagggggtta aacaaaaaaa tattcctcct ctctgaggag gaggtttcta
     1381 tcgatttgtt caaatctata aaagagatgc ctttcggcaa aagaaagatc tatagtttag
     1441 cttgcctttt atcagctgtt tctgatgagg aagctttata tacttcgata tcgatagctt
     1501 cttctcttag tttttctgat cagataagga agattgttga aaaaaacatc gagaagagat
     1561 ggcgtctttc tgatatttca aataacttga atttatcaga aatagctgtt agaaaacgat
     1621 tggagagtga aaaattaaca tttcaacaaa tccttcttga tattcgcatg catcatgcag
     1681 caaagctttt attgaatagt caaagctata ttaatgatgt atcaagactt atcggaatat
     1741 caagcccatc ttattttata aggaaattta atgaatatta tggtataact ccaaagaaat
     1801 tttacttata tcataaaaaa ttttaa
//
LOCUS       DAEBXN010000309         1824 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.314, whole genome shotgun sequence.
ACCESSION   DAEBXN010000309 DAEBXN010000000
VERSION     DAEBXN010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.314"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..>1824
                     /locus_tag="KJL99_004556"
     rRNA            <1..>1824
                     /locus_tag="KJL99_004556"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          460 a          399 c          582 g          383 t
ORIGIN      
        1 catggttaag tgggaaacga tgtgggaagg cccagacagc caggatgttg gcttagaagc
       61 agccatcatt taaagaaagc gtaatagctc actggtcgag tcggcctgcg cggaagatgt
      121 aacggggcta aaccatgcac cgaagctgcg gcagcgacgc ttatgcgttg ttgggtaggg
      181 gagcgttctg taagcctgtg aaggtgtgct gtgaggtatg ctggaggtat cagaagtgcg
      241 aatgctgaca taagtaacga taaagcgggt gaaaagcccg ctcgccggaa gaccaagggt
      301 tcctgtccaa cgttaatcgg ggcagggtga gtcgacccct aaggcgaggc cgaaaggcgt
      361 agtcgatggg aaacaggtta atattcctgt acttggtgtt actgcgaagg ggggacggag
      421 aaggctatgt tggccgggcg acggttgtcc cggtttaagc gtgtaggctg gttttccagg
      481 caaatccgga aaatcaaggc cgaggcgtga tgacgaggca ctacggtgct gaagcaacaa
      541 atgccctgct tccaggaaaa gcctctaagc atcaggtaac atcaaatcgt accccaaacc
      601 gacacaggtg gtcaggtaga gaataccaag gcgcttgaga gaactcgggt gaaggaacta
      661 ggcaaaatgg tgccgtaact tcgggagaag gcacgctgat acgtaggtga agcgacttgc
      721 tcgtggagct gaaatcagtc gaagatacca gctggctgca actgtttatt aaaaacacag
      781 cactgtgcaa acacgaaagt ggacgtatac ggtgtgacgc ctgcccggtg ccggaaggtt
      841 aattgatggg gttagcgcaa gcgaagctct tgatcgaagc cccggtaaac ggcggccgta
      901 actataacgg tcctaaggta gcgaaattcc ttgtcgggta agttccgacc tgcacgaatg
      961 gcgtaatgat ggccaggctg tctccacccg agactcagtg aaattgaact cgctgtgaag
     1021 atgcagtgta cccgcggcaa gacggaaaga ccccgtgaac ctttactata gcttgacact
     1081 gaacattgag ccttgatgtg taggataggt gggaggcttt gaagtgtgga cgccagtctg
     1141 catggagccg accttgaaat accacccttt aatgtttgat gttctaacgt ggacccgtga
     1201 tccgggttgc ggacagtgtc tggtgggtag tttgactggg gcggtctcct cctaaagagt
     1261 aacggaggag cacgaaggtt ggctaatcct ggtcggacat caggaggtta gtgcaatggc
     1321 ataagccagc ttgactgcga gcgtgacggc gcgagcaggt gcgaaagcag gtcatagtga
     1381 tccggtggtt ctgaatggaa gggccatcgc tcaacggata aaaggtactc cggggataac
     1441 aggctgatac cgcccaagag ttcatatcga cggcggtgtt tggcacctcg atgtcggctc
     1501 atcacatcct ggggctgaag taggtcccaa gggtatggct gttcgccatt taaagtggta
     1561 cgcgagctgg gtttagaacg tcgtgagaca gttcggtccc tatctgccgt gggcgctgga
     1621 gaactgaggg gggctgctcc tagtacgaga ggaccggagt ggacgcatca ctggtgttcg
     1681 ggttgtcatg ccaatggcac tgcccggtag ctaaatgcgg aagagataag tgctgaaagc
     1741 atctaagcac gaaacttgcc ccgagatgag ttctccctga ccctttaagg gtcctgaagg
     1801 aacgttgaag acgacgacgt tgat
//
LOCUS       DAEBXN010000310         1822 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.315, whole genome shotgun sequence.
ACCESSION   DAEBXN010000310 DAEBXN010000000
VERSION     DAEBXN010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.315"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            963..1589
                     /locus_tag="KJL99_004557"
     CDS             963..1589
                     /locus_tag="KJL99_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001381689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="firmicute plasmid replication protein RepL"
                     /protein_id="HBD4438991.1"
                     /translation="MYTQTCLRVLFRNVRYKFRKVRNNVAGKDASMLEQQFEDFEQQK
                     RRDHQAQDQSKSAKQKRDRLNPPDWSQVTKAGWNMLDSISEKVPRAFRLYSFFAQNID
                     KSCGAVVCDQKFLADYFGVSLRTIQYWIGDLESIGAIVKIPVGIYYAYALDPYQVWRG
                     YNKSKKYAAFNAKTLTQKDGVIDRKLRLMLSRREEFEGSLKTSNHDLD"
BASE COUNT          555 a          374 c          437 g          456 t
ORIGIN      
        1 aaaaaaaagt gcgaagaaaa aaattcctgc acctgtaatt gagttagata aatatcggaa
       61 aaagtgatgg ccatacagag ccagcggcag gcggacaatc cacagaacat tgcatgcaat
      121 ttctagttga ttgtctatcc tgcattgctc ttttgccgcc tcaaaatccc ttgcgtcttt
      181 ttgctccccg ttttccagaa cgaccgagcc gccacggttc cggcagcgcc ttgagcgcaa
      241 ccggaaaccg tctggggaga accccagacc cccatcgctg taacatcgac ggtctacgac
      301 cacctaccaa ccgcgtggct gcctattccg tgcaaatggg ctttgcaggg ggctggcgcg
      361 gttcctggcg acctgaaatc ccctggttag gggtatcggc gcgcctgacc tcctaaaacc
      421 ctctgtaagc gtttttcgcg ccttccatgc cctgatgggc atttaaaacc cgtttcgtgc
      481 gtctgagagc ttctgagagc gtttgaagag ctatctggcg actcatcccc gaaagaacaa
      541 cagcggaatc accgataaca tgcgagaggt ggcaccgctc gccgcagccc cagcgaccga
      601 acgttagtga gcgagtgggc gaggaagcgg aagagaaccg gaaaccacat tgagcacgta
      661 cgcactgatg cggggcgttt tagcggtgaa cggactgcaa aaacatgatc gagctgttga
      721 acgtggagag cgcgaaatcg ggcaaaatca cggctgacga ggcttgcaga agagtaagcc
      781 agtatacact ccgctatcgc tacgtgactg ggtcagggct tcgccccgac accccaaatg
      841 ttcgatgaac taattaaatc ttgtgtagca aaaaagtgct ttgatagatc tttagagcaa
      901 aaatcgacat tctcttaaaa atcaaagcat tatgacacca acatacgaaa cgttatttgc
      961 atgtgtatac gcaaacatgt ttgcgtgtgt tgtttcgtaa tgtacgatat aaatttcgta
     1021 aggtacgaaa caacgttgca ggtaaggatg caagtatgct ggaacagcaa tttgaagatt
     1081 tcgaacagca aaaacggcga gatcatcagg cacaagatca atccaaaagt gctaagcaaa
     1141 aacgagatcg gttgaatcca cctgactggt cgcaggttac taaggcgggc tggaatatgt
     1201 tggactctat atctgagaag gttcccagag catttaggct atattctttt tttgctcaaa
     1261 atatagataa atcgtgtggg gctgttgtat gtgaccaaaa attcttagct gattatttcg
     1321 gtgttagttt aaggacgatc caatattgga ttggagattt agaaagtatt ggtgccatcg
     1381 tgaaaatacc agtaggtatc tattatgctt atgcgttaga tccgtatcag gtatggcgcg
     1441 gatataataa atctaaaaaa tatgcagcgt ttaatgcaaa aacattaact caaaaagatg
     1501 gcgttattga tcgcaagttg cggctcatgc tctctaggag agaagagttt gaaggctcgc
     1561 ttaagacatc taatcatgac ttggactagg tatgatgcgc tatgagaatt aaatatgaat
     1621 cacgttggaa attagaagaa ctaaaattag aaatatgtag atcaatagat tcgttaattg
     1681 atgaatatca tatcgaatat gcaatgggaa taaatctata tatgaattgt gaagataagg
     1741 agtttaatcc cgttgatatt gtcaatgaag atggaaagaa tattatgttt gtcgttagca
     1801 acaatgaaaa aatacaaaaa cc
//
LOCUS       DAEBXN010000311         1809 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.316, whole genome shotgun sequence.
ACCESSION   DAEBXN010000311 DAEBXN010000000
VERSION     DAEBXN010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.316"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            173..1198
                     /locus_tag="KJL99_004558"
     CDS             173..1198
                     /locus_tag="KJL99_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBD4438992.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPRFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVSNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            1266..1809
                     /locus_tag="KJL99_004559"
                     /pseudo
     CDS             1266..1809
                     /locus_tag="KJL99_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc.;
                     frameshifted; incomplete; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
BASE COUNT          421 a          421 c          462 g          505 t
ORIGIN      
        1 caccattgcg gggcgctgtt tttattattt cctgtctaca ctcagcttat tgcgacgcga
       61 aatcgtgcgc aaaagctggc actacttttg cttataagag gatggccaca gacaggtaaa
      121 agacgtttcc caaacgtcct ataacgataa ttttcgcctc acaggaagca ttatgaaaaa
      181 gatgatgata gccacactgg ctgccgccag cgtgctgctt gccgttgcaa atcaggcgca
      241 tgctggcgcg acgcttgatg ccgttcagaa aaaaggtttt gtgcaatgcg ggatcagtga
      301 tggattacct aggttctctt atgccgatgc agacggtaag ttttcaggta ttgatgttga
      361 tgtttgtcgt ggtgttgccg ctgctgtatt tggtgacgac acgaaagtga aatatacccc
      421 actcacagca aaagaacgct tcaccgcttt acagtcaggg gaagtggatt tgctctcccg
      481 taatacgacc tggacttcat ctcgcgatgc cgggatggga atggcattta ccggcgtcac
      541 ttattacgac ggcattggct tcctgacgca cgataaagcg gggctaaaaa gcgcgaaaga
      601 actggatggc gctaccgtct gtattcaggc gggtactgat accgaactca acgtcgccga
      661 ctatttcaag gcaaacaata tgaagtacac accagtgact ttcgatcgct ctgacgaatc
      721 agcgaaggca ctggaatctg gtcgctgcga tacgctggct tcggatcagt cgcaactgta
      781 tgccttgcgc atcaaattaa gcaacccggc tgaatggatt gtcttaccgg aggttatctc
      841 taaagaaccg cttgggccgg tagttcgtcg tggcgatgat gaatggttct cgattgtacg
      901 ctggacgctt ttcgccatgc taaatgctga agagatgggc atcaattccc agaacgtcga
      961 tgaaaaagcg gctaatccag caacgcctga tatggcacat ctgttgggta aagaaggcga
     1021 ttacggcaag gatctgaagt tggataataa atgggcctat aacatcatca aacaggtgag
     1081 taactactcg gaaatttttg agcgtaacgt aggttcagaa agcccgctga aaattaaacg
     1141 tgggcaaaat aatctctgga ataacggcgg tattcagtac gcgccgcccg tgcgttaagt
     1201 cggtgtgagg taacgagcgt cgctacggcg gcgctctctt cagcgttatg ctttccgagg
     1261 tttctatgcc tcatcgccgc tcaaccgtta aaggctcact ctcctttgcc aaccctacgg
     1321 ttcgtgcctg gttattccag atccttgccg ttgttgctgt tgtcggcatt gttggttggt
     1381 tatttcaaaa cactgtaacg aatctcaata atcgtggcat tacttcaggt tttgcctttc
     1441 tggatcgcgg cgctggcttc ggtattgtcc agcatttgat cgattaccag cagggcgaca
     1501 cctacggacg cgtttttatt gtcggcttac tcaatacgct actggtttct gcattgtgta
     1561 ttgtgttcgc ttctgttttg ggcttcttta tcggtctggc gagactttcg gataactggc
     1621 tgctacgaaa gctttccaca atttatattg agatcttccg taatattgca aatcttcttc
     1681 tggtactttg ccgtgttgcg caatttgccc ggaccacgcc aagctgtgag cgcgtttgat
     1741 ctggcctttt tgagcaatcg tgggctttat attccgtcac cgcagctggg agacggattt
     1801 attgcgttt
//
LOCUS       DAEBXN010000312         1768 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.317, whole genome shotgun sequence.
ACCESSION   DAEBXN010000312 DAEBXN010000000
VERSION     DAEBXN010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.317"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            8..658
                     /locus_tag="KJL99_004560"
     CDS             8..658
                     /locus_tag="KJL99_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4438993.1"
                     /translation="MHRIDTKTAKKDKFGAGKNGFTRGNPQTGTPATDLDDDYFDMLQ
                     EELCSVVEASGASLEKGRHDQLLTALRALLLSRKNPFGDIKSDGTVKTALENLGLGEA
                     AKRNVGTGANQIPDMSLFASINTVTAAAQKFPSGLILQCGQLNGAPNVSSTYGMRFPM
                     TFSRVIAVVVTLNVTGAAGQPTVSATSVQNTGFNITVSPGSGYGSSADAYYIAMGY"
     gene            630..1064
                     /locus_tag="KJL99_004561"
     CDS             630..1064
                     /locus_tag="KJL99_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HBD4438994.1"
                     /translation="MRITLQWDINKMSYFYSASTNGFYSTEFHGTNIPDDAVEISESE
                     WKTLINAQSVTKMITCGENGHPVIVDRPSPTPEQLALINDEKKSALIAEATNVIAPLQ
                     DAVDLGMATDDETKLLLAWKKYRVLLMRVNVVKPEWPMHPNK"
BASE COUNT          495 a          350 c          408 g          515 t
ORIGIN      
        1 gtaatccatg catcgtatag acacgaaaac cgcgaagaag gataagttcg gcgcgggtaa
       61 gaacggtttt acccgtggta acccccagac tggcacgcct gccaccgatc tggatgatga
      121 ctactttgac atgttgcagg aggagctttg cagcgtggtg gaggcatccg gtgccagcct
      181 ggagaagggg cggcacgatc agttgcttac cgcacttcgc gcgctgctgt taagccgcaa
      241 gaatccgttt ggcgatatca aatcggatgg cacggtgaaa acggctctcg aaaaccttgg
      301 tttgggagaa gcagctaaaa ggaatgtagg tacaggggcg aatcagatac ctgatatgag
      361 cctgttcgcg tcaattaata ccgtaacggc tgctgcgcaa aaatttccgt ctggattaat
      421 tttacagtgc ggtcagttga atggtgcccc gaatgtatct tcaacatacg ggatgaggtt
      481 cccgatgacg ttctcaagag taattgctgt cgtagttaca ttgaacgtta ctggcgcggc
      541 tgggcagccg actgtatcgg cgacaagtgt ccagaacact ggatttaata ttactgtgtc
      601 gcccggttca ggatacggtt catctgctga tgcgtattac attgcaatgg gatattaaca
      661 aaatgtcata tttttattct gcatcgacaa acggatttta ttcgactgaa tttcacggca
      721 ccaatattcc tgatgatgca gtggaaatct cggaatcaga gtggaaaaca ctgattaatg
      781 cacagagtgt aacaaaaatg attacctgtg gtgagaacgg tcatcctgtc attgttgacc
      841 gtccttctcc aacaccagaa caattagcct taataaatga tgaaaagaaa tctgcactga
      901 tagcagaggc aacgaatgta atagctccgc ttcaggatgc ggttgattta ggtatggcaa
      961 cagatgatga aacgaaactg ttactggcat ggaaaaaata tcgggtgctg cttatgcgtg
     1021 ttaatgtagt aaaacccgag tggcctatgc atccaaataa atagacttgt taatctagga
     1081 taattgttgg aataatactc gtttttcagt aggttcaggt agcctgggta taatgctcac
     1141 ttctacaatg atttcaactt attttattta attgatatta cgaaacttaa ctcatacata
     1201 aacaatatga ctcaatactg tcctaaatag ctgcgcagaa aagtgctcca ctttcgctca
     1261 gcccttgctg ggcgaagcat caggaaggcg ttctcagcta ggttgtgtta tatctggctt
     1321 gagttttgtt gcccatacgc aacacattac ccatctgtaa acgacaagtg tggcagaatt
     1381 aggtgcagct atttagacta tatcgacgca gtaacgataa tagatatcga aactgtacta
     1441 gatattagct acttatggtt accgggggga gtcttttgag cactggtgtt atcaattggc
     1501 ttcaaataat tcgccaataa attatattgc gatagctcat caccttccag gttgtttata
     1561 gccagtttat cagcgtcacc acctactgtt tatgctttta tttctttgat taataagtat
     1621 atgatatttt aattcatctg gtgtatttct ttctcatgtg ttggtcgact gaatatctgt
     1681 tcagtgaagg ggtaatttta tgtttgcact tcgaacattc ccttcagttg ctaggaaaga
     1741 aaaccgtaac atttgcgcat taatttta
//
LOCUS       DAEBXN010000313         1760 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.318, whole genome shotgun sequence.
ACCESSION   DAEBXN010000313 DAEBXN010000000
VERSION     DAEBXN010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.318"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(1..264)
                     /locus_tag="KJL99_004562"
                     /pseudo
     CDS             complement(1..264)
                     /locus_tag="KJL99_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(379..1017)
                     /gene="ospI"
                     /locus_tag="KJL99_004563"
     CDS             complement(379..1017)
                     /gene="ospI"
                     /locus_tag="KJL99_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspI"
                     /protein_id="HBD4438995.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
     gene            complement(1176..1646)
                     /locus_tag="KJL99_004564"
                     /pseudo
     CDS             complement(1176..1646)
                     /locus_tag="KJL99_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          502 a          434 c          319 g          505 t
ORIGIN      
        1 cagcaagcgc tgtaactctt ttatttgttt tatagcggca gccagttctg aggcaggaac
       61 gacatcctct ccggcagcaa cagcagtgag acttccttcc agatattatt ttttccactt
      121 aagcaacagg ctgggctgaa taccatgcaa acgcgccaca tgggacaccg tcatgccagg
      181 ctccattgtc tgctgaataa tggcaatttt ttcctgagga gttttacgtt tacgaagctc
      241 cagccccagc aaagttccag tcatctcaaa attagaacta gtgttagaca tatatccaag
      301 cctatctctt attttaagat acatccactg tctggcgttt caaggggccg gctcaaatct
      361 atgtacaggc tctcagcatc agcaaagcct cttacttttc caactactac tgttagcata
      421 gtgatcaaat tcctgatcag atacatgaat tacctgaacc gaactatttt catctacaaa
      481 tgcatctggc gatttttcta caggatcata aagattaaat ccctttcctg tttgaacatc
      541 caaaaacata attttttcac catcgttaaa tccagctatc caatgagtgg cctgttcaat
      601 agaaatcatt actacacttc cttttgaagt attttgtaaa atattttctg caatacgctc
      661 acgaaattta ctataagttt cattaggatg tgacactatg ccttcactat caatttgctc
      721 taaatgccta tgaaaaaatc cggtcatata ctctgatatt tcattaggta cgggagcctc
      781 tcttactcca taccctaaca tagccataca agcatgaagc gcacaccctg aacagttacc
      841 atccccacca gacggattta tcattttctg taaagtcgct gccaattcag aatctttatc
      901 catatcacca agacttttag agtttaaaga ttccagcaat tttttaacat ttacatttcc
      961 ctcctctgta tgtgcctcat atccagcagt tccctgtaac gacaccccat taatcatatt
     1021 gtatcctgac agtatatata ctcactatat tttcttcata acaaatatcc ctctgatatg
     1081 acaacaaaaa acggagccgg attcctgttt ttgtgaactg cagctattcg tgtctaaaaa
     1141 gtgatccact taccaccaac ggcctaatat atacagaacg gagtaaacag gactaacagt
     1201 tcccgacagg tctcatcatt ccgtggcccg aaagtgtaag ccaacacacc gccagtttta
     1261 gtgttgtacg aataccagag ccagtgttgt tgcagccttg ctgccaacga agcgccactg
     1321 ctcatcaagt tcacagataa gggcaacatc agcgtgagcg actggcgcag atgttatccg
     1381 ccggggcggg agctttttaa tgtccgtatg acggtattga tgccgatttt cagtgtcctg
     1441 gcggtatcac ggccccctgc gccgttatgt gccatttcaa cgatctgctc cttaacgccg
     1501 ggttttctgg cttcataaga gtaagtgagc tggaaaacac gtttacatga gcgacaacgg
     1561 aaacgctcat gccgggaaca actacggcca tgtcgataaa cttcatcgga atgacagcgc
     1621 ggacaatgaa cagtaacggt agccatggaa aaccttaaat accagtagat taccagaaac
     1681 ttctgtcgat tgaagacatc acccgaagtt tgaattcatt cgaatacttt ttacgaggtt
     1741 cttttcgcca gtcctgtaat
//
LOCUS       DAEBXN010000314         1751 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.319, whole genome shotgun sequence.
ACCESSION   DAEBXN010000314 DAEBXN010000000
VERSION     DAEBXN010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.319"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            455..1303
                     /locus_tag="KJL99_004565"
     CDS             455..1303
                     /locus_tag="KJL99_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HBD4438996.1"
                     /translation="MLNVAIENQNGWNYSAPAPHKTGAGIATPTMTTAHNRAQAVFLC
                     VKHSHIQIMVGRAGQPQGWPVSVVTGCSNPVRLTTHEIATSGGESFKLTIEAAIMATI
                     LTLSHPDATIENGRAVTTSVAVAEFFRKMHKNVIQKIETLECSPEFNRLNFKPVTYTD
                     AKGEKRPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEAELRQNNTPPADKMI
                     PGDGRTLVVHFDKFGNVEFTETVPDGALVCTLETFRFYLEKQGWTLVNRGAIKNMTVE
                     QLLSLK"
     gene            1320..1601
                     /locus_tag="KJL99_004566"
                     /pseudo
     CDS             1320..1601
                     /locus_tag="KJL99_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          501 a          399 c          411 g          440 t
ORIGIN      
        1 cagcattata tagttctact ctggcgagat acgttggcat attcatttcc ttactggttg
       61 tgtgagaact tcagtaaaga taccaccaaa gcccggaagt ggtgaaataa aaccgggcac
      121 aacacgaagg cgcatttccg gtatccataa agagtcggtc ttgtctgtga aatttaaatg
      181 gtgggagtgc gcttccggtt gtaaataacg acattgctgt gtgaagtacc agttggcggc
      241 atcggtttaa ttgctggctg atgtccgccc tttttaaagt gaattttgtg atgcggtgaa
      301 tgcggctatg cgcacgcggc acagttaaaa gtcatgttag tccttattgg tttgggtggg
      361 aaagccaact gtaattgtta actggttaac gtcacctgga ggcaccaggc accgcatcaa
      421 caaagttcac ttcggtgatg aaaggaaaga gaaaatgttg aatgtagcta ttgaaaacca
      481 gaacgggtgg aattatagtg cacctgcacc tcataaaacg ggtgccggga ttgctacccc
      541 gacgatgact actgcgcata accgcgctca ggcggttttt ttatgcgtca agcacagcca
      601 cattcagatt atggtggggc gtgcagggca gccgcaaggc tggccggttt cggtagtcac
      661 cggttgtagc aaccctgtgc gtctcaccac ccatgagatt gctacctctg gtggtgagtc
      721 atttaaactg actatcgagg ctgccatcat ggctactatc cttacccttt ctcaccctga
      781 cgcaaccatc gaaaatggtc gcgctgtcac tacgtctgtt gcggttgcgg agttcttccg
      841 gaaaatgcac aaaaacgtta ttcagaagat cgaaactctc gaatgctcgc cggagtttaa
      901 ccggcttaat tttaagcccg tcacctatac cgacgccaaa ggcgaaaaac gcccaatgta
      961 ccaaatcacc aaaaacggct tcgttttcct ggtgatgggc ttcactggca aaaaagccgc
     1021 cgcattcaag gaagcctaca ttgctgagtt cgaccgcatg gaggccgaac tgcgccagaa
     1081 taacacccca cctgcagaca agatgattcc gggtgatggt cgcactctgg ttgttcactt
     1141 cgacaaattc ggcaatgtcg aattcaccga aaccgttcct gatggcgcac tggtctgtac
     1201 cctggagact ttccggtttt atctggaaaa acagggatgg actcttgtta accggggcgc
     1261 aattaaaaat atgactgtgg aacaattact ttctttaaaa taaaaatgga gctacggata
     1321 tgaaccctat gcttgattat tgttttacct ctgcacctca gcagaccata acacacaccg
     1381 gaatcaaaaa tttgttatgg ataatcaaaa aaaacctcca gtagaaaggt gatcccgatt
     1441 taattccctg tgcttatggc ctgtgtgaca gacaagtaag ccctgataag gcttacactg
     1501 ctgaacttat ttacatgcac ggaaccatga tgaggaaaaa gaaacgcaaa tattgtagtg
     1561 aaatctgcgc cgaaaaagac cagatggcac acgaacttta attaactgac tattcgaaac
     1621 tgaatttatg ccagcaatgg cagggattcg ctcaacctta attaaggaga aaaacatgaa
     1681 caccaattat gaagccactg ttgcaactac tgataacatt gttcacgagg tttacctgga
     1741 aggaaagcat a
//
LOCUS       DAEBXN010000315         1734 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.320, whole genome shotgun sequence.
ACCESSION   DAEBXN010000315 DAEBXN010000000
VERSION     DAEBXN010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.320"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            70..165
                     /locus_tag="KJL99_004567"
     CDS             70..165
                     /locus_tag="KJL99_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HBD4438997.1"
                     /translation="MLVFQMRYQMRYVDKTSTVLKQTKKSDYADK"
     gene            291..1028
                     /gene="erm(B)"
                     /locus_tag="KJL99_004568"
     CDS             291..1028
                     /gene="erm(B)"
                     /locus_tag="KJL99_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HBD4438998.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPRVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            973..1143
                     /locus_tag="KJL99_004569"
     CDS             973..1143
                     /locus_tag="KJL99_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine methyltransferase"
                     /protein_id="HBD4438999.1"
                     /translation="MSKYCLFLIVIYYLTGGNNSMSRFCKFGKLHVTKGNVDKLLGIL
                     LTASKELKRSLD"
BASE COUNT          641 a          288 c          278 g          527 t
ORIGIN      
        1 attttgtata ataggaattg aagttaaatt agatgctaaa aatttgtaat taagaaggag
       61 ggattcgtca tgttggtatt ccaaatgcgt tatcaaatgc gttatgtaga taaaacatct
      121 actgttttga aacagactaa aaaaagtgat tacgcagata aataaatacg ttagattaat
      181 tcctaccagt gactaatctt atgacttttt aaacagataa ctaaaattac aaacaaatcg
      241 tttaacttct gtatttgttt atagatgtaa tcacttcagg agtaaattac atgaacaaaa
      301 atataaaata ttctcaaaac tttttaacga gtgaaaaagt actcaaccaa ataataaaac
      361 aattgaattt aaaagaaacc gataccgttt acgaaattgg aacaggtaaa gggcatttaa
      421 cgacgaaact ggctaaaata agtaaacagg taacgtctat tgaattagac agtcatctat
      481 tcaacttatc gtcagaaaaa ttaaaactga atactcgtgt cactttaatt caccaagata
      541 ttctacagtt tcaattccct aacaaacaga ggtataaaat tgttgggaat attccttacc
      601 atttaagcac acaaattatt aaaaaagtgg tttttgaaag ccatgcgtct gacatctatc
      661 tgattgttga agaaggattc tacaagcgta ccttggatat tcaccgaaca ctagggttgc
      721 tcttgcacac tcaagtctcg attcagcaat tgcttaagct gccagcggaa tgctttcatc
      781 ctaaaccaag agtaaacagt gtcttaataa aacttacccg ccataccaca gatgttccag
      841 ataaatattg gaagctatat acgtactttg tttcaaaatg ggtcaatcga gaatatcgtc
      901 aactgtttac taaaaatcag tttcatcaag caatgaaaca cgccaaagta aacaatttaa
      961 gtaccgttac ttatgagcaa gtattgtcta tttttaatag ttatctatta tttaacggga
     1021 ggaaataatt ctatgagtcg cttttgtaaa tttggaaagt tacacgttac taaagggaat
     1081 gtagataaat tattaggtat actactgaca gcttccaagg agctaaagag gtccctagac
     1141 tagcaagaag tacacaagaa gccttaaaga ttatagaaaa gcttttccat gaaatgctta
     1201 acattattta atgttaacat gtggctttgt ggtaactacc acgagtggta acttgtaaca
     1261 tgaaaaaagt tacaagttac aagtggttta tttacgaaca taaaggctag gaaagcactt
     1321 gataagtata tcaagtgctt tttttccgtt cgattttact atccaagata ccgcttgacg
     1381 tacctaatat tttagtgtgc taagggcttt gctttttctg gttatagttc gttcaaaaaa
     1441 gaccgcaagg gtaaatgcac gatcatagat cgcccttgca gctttttaat ctctccactt
     1501 ttttataacc aagcaaacca ccaagcacac tgaaaaaata attaggaact aaagcgaagt
     1561 gcgtatcttg gataaatctt cactcgattt taaaagatta agcacccagc caaagcgcct
     1621 aaaaaaataa tttttttaaa gaaaggaagt aatttaaatg actacaattt taagcgacta
     1681 caataaatct atcctaattg aattactaaa caccaatcgc caaattatcg tcgt
//
LOCUS       DAEBXN010000316         1730 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.321, whole genome shotgun sequence.
ACCESSION   DAEBXN010000316 DAEBXN010000000
VERSION     DAEBXN010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.321"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..633
                     /gene="ipaH9.8"
                     /locus_tag="KJL99_004570"
     CDS             <1..633
                     /gene="ipaH9.8"
                     /locus_tag="KJL99_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HBD4439000.1"
                     /translation="SCEDRVALTWNNLRKTLLVHQASEGLFDNDTGALLSLGREMFRL
                     EILEDIARDKVRTLHFVDEIEVYLAFQTMLAEKLQLSTAVKEMRFYGVSGVTANDLRT
                     AEAMVRSREENEFTDWFSLWGPWHAVLKRTEADRWALAEEQKYEMLENEYPQRVADRL
                     KASGLSGDADAEREAGAQVMRETEQQIYRQLTDEVLALRLPENGSQLHHS"
     gene            complement(813..1436)
                     /locus_tag="KJL99_004571"
     CDS             complement(813..1436)
                     /locus_tag="KJL99_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HBD4439001.1"
                     /translation="MHMSLAQCPGFLFAHREECTVEIKKIINPRYTESGAVDCDVFFD
                     DRDQAVPYTATADDVAPTGQQIWQELQSGKWGEIAPFTVTPEMLEAAREARRQEIEAW
                     RTEQEAKPFTFEWNGRTWNADASSVARLSPVVMLAKSVAAQTHMVWSDADNQQVKLSM
                     PELEELAAAMVQAQVDRNDEIYRRQREMKEELSSLDDLASIRAFDVK"
     gene            complement(1387..>1730)
                     /locus_tag="KJL99_004572"
     CDS             complement(1387..>1730)
                     /locus_tag="KJL99_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBD4439002.1"
                     /translation="ITTEDIIYRIVASSVRNRWGDPDIGGLIIAAYQGEADGDKVIRL
                     VRGQSYRGSRLGPVGISVPGTPTGTYIASPQFFITGCSEHSLPGSYCALSGVPDAHVS
                     GAMPGLFIRTS"
BASE COUNT          388 a          498 c          444 g          400 t
ORIGIN      
        1 agctgtgagg accgtgtcgc gctcacatgg aacaatctcc ggaaaaccct cctggtccat
       61 caggcatctg aaggcctttt cgataatgat accggcgctc tgctctccct gggcagggaa
      121 atgttccgcc tcgaaattct ggaggacatt gcccgggata aagtcagaac tctccatttt
      181 gtggatgaga tagaagtcta cctggccttc cagaccatgc tcgcagagaa acttcagctc
      241 tccactgccg tgaaggaaat gcgtttctat ggcgtgtcgg gagtgacagc aaatgacctc
      301 cgcactgccg aagccatggt cagaagccgt gaagagaatg aatttacgga ctggttctcc
      361 ctctggggac catggcatgc tgtactgaag cgtacggaag ctgaccgctg ggcgctggca
      421 gaagagcaga aatatgagat gctggagaat gagtaccctc agagggtggc tgaccggctg
      481 aaagcatcag gtctgagcgg tgatgcggat gcggagaggg aagccggtgc acaggtgatg
      541 cgtgagactg aacagcagat ttaccgtcag ctgactgacg aggtactggc cctgcgattg
      601 cctgaaaacg gctcacaact gcaccattca taatcacatc gcataaacca cagaccggac
      661 tgactccgga aaaacagagg cccgcccccg ggcctccccg gattcatccg tttctctgtt
      721 cagcctgacc gcacaccccg gcggccggat gacagactcc gcctcggtaa gcaaagcggt
      781 cttctgtgat tccgccagtt gcggcttatt cattacttaa cgtcaaacgc ccgaattgaa
      841 gccaaatcat ccagactgct cagctcctct ttcatctccc gctgacggcg ataaatctca
      901 tcgttgcgat cgacctgcgc ctgcaccatt gctgccgcca gttcttccag ttccggcatc
      961 gacagtttca cctgctgatt atccgcatca ctccacacca tatgtgtttg tgccgcgaca
     1021 gattttgcca gcatgaccac cggggacagg cgggccactg acgaggcgtc agcgttccac
     1081 gtgcggccgt tccattcaaa cgtgaacggc ttcgcctcct gttctgtgcg ccatgcttca
     1141 atttcctgac gtctggcctc tctggccgct tccagcattt ctggtgtcac agtgaatggg
     1201 gctatctcac cccatttgcc actttgcagt tcctgccaga tttgctgacc cgtcggtgca
     1261 acatcatcag cggtggctgt gtagggaact gcctggtccc tgtcgtcaaa aaaaacgtca
     1321 cagtctactg cgccactttc ggtataacgg ggattaatga tttttttaat ttccacggtg
     1381 cattcctcac gatgtgcgaa taaaaagccc gggcattgcg ccagagacat gtgcatccgg
     1441 caccccggac agggcgcaat atgaccccgg taatgaatgc tctgaacatc ccgtaatgaa
     1501 aaattgtggg gatgctatat acgttccggt gggagtaccg ggcactgaaa tccccaccgg
     1561 tcccagtcgt gagcctctgt atgactgccc cctgacaagt ctgatgactt tatcaccgtc
     1621 agcttctccc tggtacgcag caataatcag cccgccaatg tcagggtctc cccatctgtt
     1681 gcggacagag ctcgccacga ttctgtaaat aatatcttct gtggttatat
//
LOCUS       DAEBXN010000317         1719 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.322, whole genome shotgun sequence.
ACCESSION   DAEBXN010000317 DAEBXN010000000
VERSION     DAEBXN010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.322"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(110..577)
                     /locus_tag="KJL99_004573"
     CDS             complement(110..577)
                     /locus_tag="KJL99_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBD4439003.1"
                     /translation="MYGTWSLSMAAIWLLRVDLLFVALFLIFISWGDILLRIISHRYL
                     LVLTVLIFLALILQHQKPNLVAAGSVLLVGFFLFSAGIIGGGDVKLLTVLSLAIDEHE
                     LANFLVAMTFCGALVVLAGLLFFRKSIRENGVPYAVPISLAFLLTYPVFPLFC"
     gene            complement(643..882)
                     /locus_tag="KJL99_004574"
     CDS             complement(643..882)
                     /locus_tag="KJL99_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HBD4439004.1"
                     /translation="MNTFITKYYGKTKQCFARFAKDERGVTAIEYALIGVAMATLLAF
                     IFGDQNSGFLGAIKDAFDAIAAAIQQVTISGTSNP"
     gene            complement(1017..1622)
                     /locus_tag="KJL99_004575"
     CDS             complement(1017..1622)
                     /locus_tag="KJL99_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBD4439005.1"
                     /translation="MSDDRFYFSLGQGVKFYPASRRITTSTGNVINLSENGYRFLLLL
                     LQGESDKQHIINEVWREQKGSVSDSSYYGQIYMLRKALDVAGLSSSLIKTIPRKGVRY
                     LGKVEKHVCENERDSDEDSSSYTNDSEAREVNTDANIPENDNSQEFSTEELLPIKHTD
                     KNIEWYRTKQWSVFISVLTVLAVCWLTTLVFLVLLFLNQDK"
BASE COUNT          550 a          378 c          305 g          486 t
ORIGIN      
        1 gcttaggcct gtgtccatat tacgtgggta ggatcacaag acctactgct atcacaatta
       61 ctgagagaaa aaaaagcatc cgatgattca tttatgctgt cctaaaaaat taacaaaaca
      121 atgggaaaac aggatatgta agcagaaaag ccaatgaaat tggtacagca taaggcacac
      181 cgttttcacg aattgatttt cgaaaaaaaa gaagacctgc cagaacaacc agagcgccgc
      241 aaaaggtcat tgcgacgaga aaattagcca gttcatgttc atcaatagct aacgaaagaa
      301 cagttaacag cttgacatca ccgccgccaa ttatccctgc ggaaaaaaga aagaagccca
      361 cgagcaaaac gctcccggct gcaaccagat tgggtttttg atgctgtaaa atgagtgcca
      421 aaaaaataag cacggttaac accagtaaat aacgatggct aataattctg agtaatatat
      481 ccccccatga aataaaaata agaaagagcg caacaaataa aagatcgact cttagtaacc
      541 atattgctgc cattgaaaga ctccatgtac catacaaggt tgctgccaga catattcctt
      601 tacgtctgac agcctctaga gagttttttt attttttata agttacggat tactcgttcc
      661 ggaaatcgtt acctgttgaa ttgctgcagc gatagcgtcg aatgcatctt taattgcgcc
      721 caggaagcct gagttctgat cgccaaagat gaaagcaagc agagttgcca tagcaacacc
      781 aatcaatgca tattcgattg cagtaacacc acgttcatcc ttcgcgaaac gcgcaaagca
      841 ctgtttagtt ttaccatagt acttagtaat aaaagtattc atgttttcct cgtatattat
      901 tatttaaatt caaaccacca gcttcatctg cgtgtcaccg gtatcccata tttttgggat
      961 gtggctataa ttatctattc ccgctattta gtcttcaaaa aaaattacat ttaatatcat
     1021 ttatcttgat taagaaatag taagactaaa aaaacaagcg tcgtcagcca gcacactgct
     1081 aacacggtaa gaacgctaat aaaaacactc cactgcttcg ttctatacca ttcaatattt
     1141 ttgtcagtat gttttattgg gagcagctct tcagtagaga attcctggct gttatcattc
     1201 tcaggaatgt ttgcatcagt gtttacttcc ctggcctcgc tatcgttggt atagctacta
     1261 ctgtcttcat cactgtcacg ctcattctca cagacatgtt tctcgacctt tcccagatat
     1321 ctgacgcctt ttcgcggaat ggttttaatt agagaactcg acaaacctgc gacgtctaat
     1381 gcctttctaa gcatgtaaat ctgcccataa taactgctgt cgcttaccga acctttctgc
     1441 tcccgccaaa cctcattgat aatatgttgc ttatcggatt ctccctgtag taaaaggagt
     1501 aaaaatcgat agccgttttc agacagattg attacattgc ctgtactggt ggtgatacgt
     1561 cgagacgctg ggtaaaactt aactccctgc cctaaagaaa aataaaaacg gtcatcgctc
     1621 ataagtaccg acccctgttt tcttcttcac atgactactc attacaactt tgtatgaagt
     1681 aagatgcaaa atcatattgt ttacattaag ttaagcaat
//
LOCUS       DAEBXN010000318         1702 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.323, whole genome shotgun sequence.
ACCESSION   DAEBXN010000318 DAEBXN010000000
VERSION     DAEBXN010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.323"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..439
                     /locus_tag="KJL99_004576"
     CDS             <1..439
                     /locus_tag="KJL99_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBD4439006.1"
                     /translation="RPQLEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGV
                     HIEFVKEHLSFTGEDSPMANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRK
                     KSLSSERIAELRQRVEAGEQKTKLAREFGISRETLYQYLRTDQ"
     gene            442..>1702
                     /locus_tag="KJL99_004577"
     CDS             442..>1702
                     /locus_tag="KJL99_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HBD4439007.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRG"
BASE COUNT          341 a          557 c          507 g          297 t
ORIGIN      
        1 gcgtccgcaa ctggaagcgc tgataagctt cgcccgcacc ggcgacaccg tggtggtgca
       61 tagcatggat cgcctggcgc gcaatctcga tgatttgcgc cggatcgtgc aaacgctgac
      121 acaacgcggc gtgcatatcg aattcgtcaa ggaacacctc agttttactg gcgaagactc
      181 tccgatggcg aacctgatgc tctcggtgat gggcgcgttc gccgagttcg agcgcgccct
      241 gatccgcgag cgtcagcgcg agggtattgc gctcgccaag caacgcgggg cttaccgtgg
      301 caggaagaaa tccctgtcgt ctgagcgtat tgccgaactg cgccaacgtg tcgaggctgg
      361 cgagcaaaag accaagcttg ctcgtgaatt cggaatcagt cgcgaaaccc tgtatcaata
      421 cttgagaacg gatcagtaaa tatgccacgt cgttccatcc tgtccgccgc cgagcgggaa
      481 agcctgctgg cgttgccgga ctccaaggac gacctgatcc gacattacac attcaacgat
      541 accgacctct cgatcatccg acagcggcgc gggccagcca atcggctggg cttcgcggtg
      601 cagctctgtt acctgcgctt tcccggcgtc atcctgggcg tcgatgaact accgttcccg
      661 cccttgttga agctggtcgc cgaccagctc aaggtcggcg tcgaaagctg gaacgagtac
      721 ggccagcggg agcagacccg gcgcgagcac ctgagcgagc tgcaaaccgt gttcggtttc
      781 cggcccttca ccatgagcca ttaccggcag gccgtccaga tgctgaccga gctggcgatg
      841 caaaccgaca aaggcatcgt gctggccagc gccttgatcg ggcacctgcg gcggcagtcg
      901 gtcattctgc ccgccctcaa cgccgtcgag cgggcgagtg ccgaggcgat cacccgtgct
      961 aaccggcgca tctacgacgc cttggccgaa ccactggcgg acgcgcatcg ccgccgcctc
     1021 gacgatctgc tcaagcgccg ggacaacggc aagacgacct ggttggcttg gttgcgccag
     1081 tctccggcca agccaaattc gcggcatatg ctggaacaca tcgaacgcct caaggcatgg
     1141 caggcactcg atctgcctac cggcatcgag cggctggttc accagaaccg cctgctcaag
     1201 attgcccgcg agggcggcca gatgacaccc gccgacctgg ccaaattcga gccgcaacgg
     1261 cgctacgcca ctctcgtggc gctggccacc gagggcatgg ccaccgtcac cgacgaaatc
     1321 atcgacctgc acgaccgcat cctgggtaag ctgtttaacg ctgccaagaa taagcatcag
     1381 cagcagttcc aggcgtcagg caaggccatc aacgccaagg tacgtctgta cgggcgcatc
     1441 ggtcaggcgc tgatcgacgc caagcaatca ggccgcgatg cgtttgccgc catcgaggcc
     1501 gtcatgtcct gggattcctt tgccgagagc gtcaccgagg cgcagaagct cgcgcaaccc
     1561 gatgacttcg atttcctgca tcgcatcggc gagagctacg ccaccctgcg ccgctatgca
     1621 ccggaattcc ttgccgtgct caagctgcgg gccgcgcccg ccgccaaaaa cgtgcttgat
     1681 gccattgagg tgctgcgcgg ca
//
LOCUS       DAEBXN010000319         1680 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.324, whole genome shotgun sequence.
ACCESSION   DAEBXN010000319 DAEBXN010000000
VERSION     DAEBXN010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.324"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(71..514)
                     /locus_tag="KJL99_004578"
     CDS             complement(71..514)
                     /locus_tag="KJL99_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBD4439008.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNEREQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
     gene            671..1600
                     /gene="metA"
                     /locus_tag="KJL99_004579"
     CDS             671..1600
                     /gene="metA"
                     /locus_tag="KJL99_004579"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HBD4439009.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYLRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
BASE COUNT          404 a          472 c          378 g          426 t
ORIGIN      
        1 ttggtgaaaa aaatgccggg tgcgacgctg gcgcgtcttc tccagcttgg gcaggcacgg
       61 cagaggcggc ttattccccc acatacgcca gattcagcaa cggatacggt ttccccaaat
      121 cgtccacctc agagcgtccc gtaaccttaa aacccacctt cttatagaac ccaaccgcct
      181 gctcattttg ctcattaacg ttggttgtca gttccggtgc catcgagagc gcatgctcca
      241 ccagcatccg acccacgccg cagccgcaca catcaggatc gataaacagc gcatccatat
      301 gctgcccact taacaacata aacccaaccg gctgctcccg ttcattaacc gcgacccaca
      361 acggtgcttc cggcagaaag gaacgcacca gctcttccag ctcggcccga tactctgctg
      421 ataaaaaatc gtgggtggca tcgacagatc gacaccaaat cgcaacgagt tcctcccctt
      481 cctcatgccg tgagcggcga atactaataa ccattttctc tccttttcgt cattcttata
      541 ttctaacgta gccttttcct tgaaactttc tcaccttcaa catgcaggct cgacattggc
      601 aaattttctg gttatcttca gctatctgga tgtctaaacg tataagcgta tgtagtgagg
      661 taatcaggtt atgccgattc gtgtgccgga cgagctaccc gccgtcaatt tcttgcgtga
      721 agaaaacgtc tttgtgatga caacttctcg tgcgtctggt caggaaattc gtccacttaa
      781 ggttctgatc cttaacctga tgccgaagaa gattgaaact gaaaatcagt ttctgcgcct
      841 gctttcaaac tcacctttgc aggtcgatat tcagctgttg cgcatcgatt cccgtgaatc
      901 gcgcaacacg cccgcagagc atctgaacaa cttctactgt aactttgaag atattcagga
      961 acagaacttt gacggtctga ttgtaaccgg tgcgccgctg ggcctggtgg agtttaatga
     1021 tgtcgcttac tggccgcaga tcaaacaggt gctggagtgg tcgaaagatc acgtcacctc
     1081 gacgctgttt gtctgctggg cggtacaggc cgcgctcaat atcctctacg gcattcctaa
     1141 gcaaactcgc accgacaaac tctctggcgt ttacgagcat catattctcc atcctcatgc
     1201 gcttctgacg cgtggctttg atgattcatt cctggcaccg cattcgcgct atgctgactt
     1261 tccggcagcg ttgattcgtg attacaccga tctggaaatt ctggcagaga cggaagaagg
     1321 ggatgcatat ctgtttgcca gcaaagataa gcgcattgcc tttgtgacgg gccatcccga
     1381 atatgatgcg caaacgctgg cgcaggaata tttacgcgat gtggaagccg gactagaccc
     1441 ggatgtaccg tataactatt tcccgcacaa cgatccgcaa aacaaaccgc gagcgagctg
     1501 gcgtagtcac ggtaatttgc tgtttaccaa ctggctcaac tattacgtct atcagatcac
     1561 gccatacgat ctacggcaca tgaatccaac gctggattaa tcttctgtga tagtcgatcg
     1621 ttaagcgatt cagcacctta cctcaggcac cttcgggtgc cttttttatt tccgaaacgc
//
LOCUS       DAEBXN010000320         1663 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.325, whole genome shotgun sequence.
ACCESSION   DAEBXN010000320 DAEBXN010000000
VERSION     DAEBXN010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.325"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(6..509)
                     /locus_tag="KJL99_004580"
     CDS             complement(6..509)
                     /locus_tag="KJL99_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HBD4439010.1"
                     /translation="MIFNRLKLAVDRVIATFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGVVMMFYALCFINQSIQQLK
                     QPAQEAS"
     gene            complement(554..1517)
                     /locus_tag="KJL99_004581"
                     /pseudo
     CDS             complement(554..1517)
                     /locus_tag="KJL99_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
BASE COUNT          473 a          358 c          336 g          496 t
ORIGIN      
        1 atccattaac tggcctcctg cgcaggttgt ttcagttgtt gaatgctttg attaataaaa
       61 cacaaggcat aaaacatcat taccactcca cttaatggca gaatgatata tacgtatccc
      121 atcggtattt gcatagcggc agatacctgt gatgttgcca gtgttttatc aattaatttc
      181 aggccaccag tcacaatgac cgaaccggca aaacctaaaa tgagaacgtt aatgactata
      241 ctaagaagta attgtttgcg cttattcagg gcgagagcaa ataaatcaat agagagatgt
      301 cgctgcgcgc cgacggtata tgcagcacct aataatccta cccagatcat caaaaagcgt
      361 gccagctcat cggttaatgt acttggctgg ttaagtacgt agcggctaaa tacttgccag
      421 actacgcata caaccagagc aagcattacg gcgacagaaa acgtggcaat gacgcggtct
      481 accgcaagtt tcaggcggtt aaatatcatt gtaagcatcc tggaaaatca gggccagagg
      541 ctggcccgga atattattga gcagcgttgt cgaatttctc taacaaagca gcttgttttg
      601 gatctttttt gaagtcgtca aacagaggtt gcacggcttt acggaatggc gctttatcca
      661 cttttacaat ttcacccccc atagctttcg cctgagcacg tgtgtcagcg tcaatttttt
      721 cccacaattt ttgttgataa gcttcggatt tcttcgccgc tgtttcgagg atttgttgct
      781 gttctggggt taatttattc catgttttgg tggagataac caaaaagtca ggaattgagg
      841 cgtgttcatc ttcagaaaaa actttggcaa tttcaatatg tcgagtctgc atccaggaag
      901 gcacgttatt ttctgcgcca tctactacac cttgctgcat cgcagtataa acttcgccaa
      961 atgaaattgg agtgggtgaa ccacccatca gttcaatcat tttgatggtg gtcggacttg
     1021 gctgaacacg aattttcagt cctttaagat cttctggctt tgtaatcggt tttttcgcat
     1081 agaaactgcg agtacccgct acataagctg ataaagcaaa aaagcctttg tcttttgttg
     1141 agtccataat ttctttacca acttcaccaa aaacaactta ataagaacgg caaattatag
     1201 atagcataaa tattatcaaa agactccaga tcactggcgg aacctttggt catatccagt
     1261 gcgccatttt gcaacaattc catagtttct cgcgcattac ccatctggct gctgggataa
     1321 atacggatca ccatattacc ttttgacagc tgttttacct ctttcgccag ctcttcaaaa
     1381 ctctgatgta caacatgact tctttccagg ttgtgtgcta actttaacgc cactttttct
     1441 gcggcgttta cgccagtgct aaccagagcc agcaaggatg cgcagactaa tgagcgggta
     1501 aatgacctca ttttcataaa aaaactccct gttgaaggat gggaattggt tgatatctaa
     1561 ttgtgcttat cagtgatttg tgcgacaaat tggttaacct gtctgacaac ttgtctatgt
     1621 ttatcacaac ttgaaataaa gtcagaaatt aaatattaaa ttt
//
LOCUS       DAEBXN010000321         1637 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.326, whole genome shotgun sequence.
ACCESSION   DAEBXN010000321 DAEBXN010000000
VERSION     DAEBXN010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.326"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(<1..616)
                     /locus_tag="KJL99_004582"
     CDS             complement(<1..616)
                     /locus_tag="KJL99_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HBD4439011.1"
                     /translation="MERRCFFIINDAIIFDPNKCSLSNVNTQDEINFPATAGCILAVL
                     LESNGGAVDRNSIIDSVLERFSFDLSNNTLNQYISLLRKSFKNIGVEENVILTIPKVG
                     FYISENITISKQSYDDSHLQEDVVEKVERKRSIPLFRILISLVVVIVIIEMFAVAFKD
                     TYSITDYPLVKSGKIDTCDLYLSENLNEIGLAKSQIEAAEISKKH"
     gene            862..1623
                     /locus_tag="KJL99_004583"
     CDS             862..1623
                     /locus_tag="KJL99_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBD4439012.1"
                     /translation="MLSKITQELLKMNNVLLMRPDDSEFMIPDDWSGLNAKGITYEQD
                     NRVTESFLPFKSFYCSPVCTTVELISQNNCFAMGIRYLLEKKRDLRLNHFKAFSHKCE
                     TKPDVLIMTGCTVADTYSIFSRIVEENIMKTMTPIVIITGHHSEDLCSLASKLGGVKI
                     ISPTEKIAKIENAINDAFNSKPKKIRFLTDILSPQQIETMKMVAQGRSVNEISAAMNI
                     SIKTVYAHKVRAINKLGIKKKVYEALFYKALIQFN"
BASE COUNT          579 a          315 c          271 g          472 t
ORIGIN      
        1 aatgcttttt cgaaatttca gcggcctcta tttgagattt agcgaggcca atctcgttaa
       61 gattctctga aagatataaa tcacaggtgt caatcttacc tgatttaacc aatggataat
      121 ccgttatact gtaggtatct ttaaaagcca cggcaaacat ttcaataata acaattacta
      181 ccacaagcga aatcaaaatg cgaaaaagag gaatactcct tttgcgctct actttttcaa
      241 caacatcttc ttgtaaatga gaatcatcat aagattgttt acttattgtt atgttttcac
      301 tgatgtaaaa gcctactttg ggtatcgtaa gaatcacatt ttcttcaaca ccaatatttt
      361 taaaactttt gcgtaacagg gatatatatt ggttcaatgt gttgtttgac agatcaaatg
      421 aaaatctctc caaaacgcta tcaatgattg agttcctgtc aaccgctcca ccattacttt
      481 ccagtaacac tgccaaaata caccccgccg tagctggaaa atttatctca tcctgggtat
      541 ttacattcga gagcgagcat ttgttcggat caaaaataat cgcgtcattg ataatgaaaa
      601 aacatctccg ctccatgcag gcaacaacac aaaataagat tttgtcattt tagcctattc
      661 ctggtggaac tgacacaaga aactaaaata caaatataac cctgaaaaag tcatataaaa
      721 cttatatcca cagaataaaa aatcaaacat gtcagtttta tcaatataaa gtatcgcaag
      781 gcgaaaagtg atgcaagaat actcctaagt aaatgaaagc gcaccacatg aaattatcaa
      841 cacatgtaat ttcatcatta ggtgctatcg aaaataactc aggagctact taagatgaat
      901 aatgtcttac tcatgagacc cgatgactca gaatttatga tacctgatga ctggtcaggg
      961 ctaaacgcaa aaggcattac atacgaacaa gataatagag tgacagagtc ttttctgcct
     1021 tttaaatctt tttattgttc accggtgtgc acgacagttg aactcatctc acaaaataat
     1081 tgttttgcta tgggtatccg ctaccttctg gaaaagaaaa gagacttaag gctaaatcac
     1141 tttaaagcct tctctcataa atgcgagaca aaacctgacg tgttaattat gaccgggtgc
     1201 accgtcgctg atacttatag tatattctca cgcattgttg aagaaaatat aatgaaaacg
     1261 atgacaccta tcgtcatcat tactgggcat cactcagaag atctttgttc tctggcatcg
     1321 aaactgggag gagttaaaat aatatcaccg acagaaaaga ttgcaaaaat agaaaacgct
     1381 atcaatgatg cttttaacag caagcctaaa aaaataagat ttttaacaga tatactctct
     1441 cctcaacaaa ttgagaccat gaaaatggtt gcgcagggta gatccgttaa tgagatttca
     1501 gccgcaatga atattagcat caagaccgtg tatgcacata aagttcgtgc tatcaataag
     1561 ctgggaatta agaaaaaagt ttatgaagcg cttttttata aagcgctaat tcagttcaac
     1621 tgatgtaaaa atcaccc
//
LOCUS       DAEBXN010000322         1602 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.327, whole genome shotgun sequence.
ACCESSION   DAEBXN010000322 DAEBXN010000000
VERSION     DAEBXN010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.327"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(334..480)
                     /locus_tag="KJL99_004584"
                     /pseudo
     CDS             complement(334..480)
                     /locus_tag="KJL99_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            534..716
                     /locus_tag="KJL99_004585"
     CDS             534..716
                     /locus_tag="KJL99_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein"
                     /protein_id="HBD4439013.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKSWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCC"
     gene            complement(817..1146)
                     /locus_tag="KJL99_004586"
     CDS             complement(817..1146)
                     /locus_tag="KJL99_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HBD4439014.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(1318..>1602)
                     /locus_tag="KJL99_004587"
     CDS             complement(1318..>1602)
                     /locus_tag="KJL99_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4439015.1"
                     /translation="HFVLLNAQKLCDTQHMMQQILLSLVHALYEGNPQPVFANTEKLN
                     DAVEELRQLLDNHHDLKVVETPIYGYVWLNMETAHQLELLSNLICRALRK"
BASE COUNT          435 a          368 c          377 g          422 t
ORIGIN      
        1 tagcaatgtc tgtcgcacaa caaccacaat gtcacaaata aaaaatgaga ataaccagtt
       61 ccctgaaggc gacgtagccc ggtacaagag taacccacgt tagcgcttgg cgacggcgga
      121 acaaagctga ttcgcgacgt aagaaagtgg caggctcata tgacggacct ctgtaatgcg
      181 gtatccaacc cgcgaatatc agcatgattc accgtcggat ttactgctac gtcgccctca
      241 gggaattaaa cctaaaaaga aaatcgtcaa aattagagat tccggtttga cacggaaagt
      301 catatcagcg tcaacggagc accgtattga cgcttattta ttggtgagaa ctacgttcca
      361 tggcaggagt tcgtcaacac ggttggaggg ccattcaggc agtatgctca gaatatggcg
      421 cagatacgct tccggatcga taccgttcag acggcaggtg cctgcttttc cggcaacaag
      481 caggaatctc agcgctgaag caggaattag aggtaaaaac gccctaccgg gccatgaatc
      541 acccggtcat tggagtagta accaaagcag atttagccag catggaacaa atatctttgg
      601 tgaaaagttg gttacgggaa gctggagcgc acaacgtgtt agtaaccagt gcagttaata
      661 acaatggagt tacagaactt tttgccttgc tgcatacaga agaaggctgt tgttaatttc
      721 gattggttct cagatgagaa tgatgacggt gatacacaga taagaatcaa tgagcaatga
      781 gggttgccgg gcaaccctca ttgaataaaa cgggaattac ttcgcttcgc cgtttttcat
      841 ttcgcccata gctttcagct ttttggagat atcgcggcgt tctttggaga gctcggcatt
      901 tttgatgatg taatcgtcaa cgcgatcttc atagtcacct ttcatgctgg cgatgatgcc
      961 ctggattgct tcaacgctca tccctggctt gatgtaatcg ctcaggttgt ccagcaacag
     1021 gacgcgcttc tggttgtcac ggatcttttt ctcaacgtcc tgaatttcac gttgcagttt
     1081 gttcttacga cggaacagac gaacaaattc cagtacgtcc tggaatgaag gcttggtagt
     1141 ttccattttt atacccctga taatgtgaga gtcggattcg tttaagcaac cgctattcgt
     1201 tagggccaac attactcata gctgccgcag atgacaatgc ttttatccct tttcactatc
     1261 atacccttta tccttgctga atcgaagcag cagcaagatg attctgaagt tcaggaatta
     1321 tttgcgcaag gcccggcaaa tcagattcga tagcaactca agctgatgtg ccgtttccat
     1381 gttcagccac acataaccat agattggtgt ttccacgact ttcaggtcat ggtggttatc
     1441 gagcaactga cgcagctctt ccacagcatc gttcaatttt tccgtattgg caaaaaccgg
     1501 ctgcggatta ccttcgtaca gcgcatgaac aaggctcagc agtatttgct gcatcatgtg
     1561 ctgggtatca caaagttttt gcgcgttcaa taacacgaaa tg
//
LOCUS       DAEBXN010000323         1601 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.328, whole genome shotgun sequence.
ACCESSION   DAEBXN010000323 DAEBXN010000000
VERSION     DAEBXN010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.328"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(<1..821)
                     /locus_tag="KJL99_004588"
     CDS             complement(<1..821)
                     /locus_tag="KJL99_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HBD4439016.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQRLTSSPDYHGP"
     gene            complement(1420..>1601)
                     /locus_tag="KJL99_004589"
     CDS             complement(1420..>1601)
                     /locus_tag="KJL99_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HBD4439017.1"
                     /translation="KEELTRRISKRYDDVEVIVKATSNDGLSVTRTADKDSAKTFVQE
                     TLKDTWESADEWFVH"
BASE COUNT          418 a          265 c          389 g          529 t
ORIGIN      
        1 gggccgtggt agtccggcga agatgttaat ctttgcaggg attgcagagc atgggatgac
       61 aatgggttat cactaatatc tattgaacat tcattcctca gattaagaat gctttccggg
      121 atatgactta tctgatttat gtcaaaataa ccgttcctta aattttccgg caggtatgga
      181 atatcggtta acatattgtc gattgcccat atggagtgaa tgttgtgagg tagagggggg
      241 agttcactaa gaagattccc tattgcgcta atctcttgta aagaaaatgg taaaggtggt
      301 aaaactgcca gcccattaca tgatacatct aatgtttcca gtagttctgg taaaacagga
      361 agagaacata attgattatt tgagacatga agctccttca aagatatggg gagtgtgggt
      421 agtgtgatta gttgattgtg ggacgcattc aataatttaa gtccttgagg caaagcaggc
      481 agttcaataa gtctgttata gctgacatta agttgtgtaa gggacgcagg caatggggag
      541 attaagctta aattattttt acttatatta attgatttaa ttcccggggg gatttcaggt
      601 aatgttgtca ggcctaattc agacaagtcc aggttcgtct cttggttttg tagacatgat
      661 actagtcgct gaaaagcgat gtctcgttgt tcttcttgta tgcggttatt tttccattca
      721 gtccaatggg ttagataatt ttcatatgcg cggctagtgt cgattgaata agtggagaac
      781 gaatttgaaa ttaatctgtg attgttattt gtcgggagca taaatatcag ggtgtattct
      841 ttatgttgag aggtgttgga ttctttttta tctgctttgc aatgttattg gttcccttca
      901 gcagtagaca ggaacttctg gaggaggcgg atatatagtt tgtgtaagca tattaactcc
      961 atatgttata tattgaagaa cttctgcttt acactatcct aaactggatc ggtttaatgt
     1021 aaaaaaacga tactttttgg gggggggcaa tttttcctta aggtatgaaa aaaggagctg
     1081 aagctatata atagctatta atgccactgg tatcagtgaa tcatgtatat aaaaaactgt
     1141 gttaaggagg ctgtccatta gcggtttttt attcttctgg aaccgcggca tctttgcgta
     1201 tctttttgtc ttctcaccgt ctggtcggtg ttttgctgag actgctaatt tcctgtcttt
     1261 gctagtgctg tactggtact gcccaatcat gattggcggg ggcgggcgga gttgagaccg
     1321 cagacacgtc gtatgcaaga acgtgctgcg gctggctggt gaacttccga tagtgcgagt
     1381 attgaatgat ttccagccgt tactgatttt acgtgctaat tagtgaacaa accactcgtc
     1441 agcagactcc caggtatctt tcagagtttc ctgaacaaaa gttttagctg aatctttatc
     1501 ggcggtgcgc gtaacagaaa ggccatcgtt gctggtggct tttacgatca cctctacatc
     1561 gtcataacgt ttactgatgc gtcgggttaa ttcttccttt a
//
LOCUS       DAEBXN010000324         1597 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.329, whole genome shotgun sequence.
ACCESSION   DAEBXN010000324 DAEBXN010000000
VERSION     DAEBXN010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.329"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(<1..636)
                     /locus_tag="KJL99_004590"
     CDS             complement(<1..636)
                     /locus_tag="KJL99_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015703070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HBD4439018.1"
                     /translation="MILSVFLGQPALTYAGVVIGSTRVVYLSNNADKSISVFSKEEKI
                     PYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPSQEKILRIIHTKGVSLPDDRESVFWL
                     NIKNIPPSASNKATNSLEIAVKTRIKLFWRPANIRLIPEDAAPKVKWRREGRNLIAEN
                     PNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPANSAGGQMTWRFINDYGA"
     gene            complement(750..1322)
                     /locus_tag="KJL99_004591"
     CDS             complement(750..1322)
                     /locus_tag="KJL99_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HBD4439019.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVNFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT          474 a          337 c          319 g          467 t
ORIGIN      
        1 tgcgccgtaa tcattaataa atcgccaggt catttggcct cccgcagaat tagcaggtaa
       61 aggtaacgtg agcgtttcaa aaggacggat catattgagg ggcacatcat ggccatcaac
      121 gatgacatcc atcacactga tatgaattgg gttgggattc tctgctatca gatttcgccc
      181 ttcccggcgc catttcactt tcggcgcagc atcttcggga ataaggcgga tatttgctgg
      241 tcgccagaac agcttgatac gcgtttttac tgctatttcc agcgagttcg tcgctttgtt
      301 gctcgctgat ggcggaatat ttttaatatt cagccagaag actgactcgc gatcatcagg
      361 caatgacaca cctttggtgt gaataatgcg taatattttt tcttgcgaag gttctaatcg
      421 tgatactggc ggaataacgg taaacggcgc tttgctttta tcctctttat taaatggatc
      481 tacccaagcc tggatgagat aaggtatttt ttcttctttg ctaaatacag aaatagattt
      541 atcagcatta ttacttaagt aaaccacccg cgtactacca atgacaacgc ctgcataagt
      601 taatgctggt tggcctaaaa aaacgctgag tataatcgcg gtaaataact tacattaaac
      661 tgttgtttta aagagcctga ttaaacaact ttttctggac ataacgactc cctggccaaa
      721 gccaactgaa taaaaaggcc cttgataaat tatttcttat attcgacagt aaattgtgaa
      781 tcagcgttcg ccgtaccagc cgttacggtc gcattggttg ccacatagcg tgcaataaac
      841 ttcatttcag ccttgccttc agcatcgata gcggtataag cagagtcgtt ataaagttta
      901 acctgcgaat tatcggactg gttaaaaata cgaataccaa cgccggttgc cgcaatcgct
      961 tgtcctgtac cggtaaaatc accagggttg cctgctgtaa gcggcgtacc cactttcagg
     1021 tcgccgtcgc cgccaggcgc ttctgtacca tcaaaacgaa cggctgcttg agtgtacgtt
     1081 gccgggcaat tttcaagttt aatactgaac ttctgtggcg acgccagact acctacacct
     1141 gaaaatgcat ttttactgac tttacccagt gggacaattt gatcggctgt atcggaggta
     1201 acttcacaag tagtgtcgac aatttcaccg acgaagttaa caacaccatc ttctgccagt
     1261 gcatgcccgg ccagcattag agatgccaga gtgaatgcac cgcctataat attacgcttc
     1321 ataaaaactc cattgatata atatatcctt taccatgaat catcacttat attaataagt
     1381 gtgtttttga ttcaaaggaa tagtacacca aagagacggc tttatctaaa aataatatta
     1441 attacgcaaa aataaaatat agatttattg tttaaatatt atttaaacag taaaaaatca
     1501 agtttgttat cagacattaa tgataattca gaatatattt atatagaaag atagatgaat
     1561 ttattgatgt cagttataaa aaagggccac cggtcgg
//
LOCUS       DAEBXN010000325         1581 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.330, whole genome shotgun sequence.
ACCESSION   DAEBXN010000325 DAEBXN010000000
VERSION     DAEBXN010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.330"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(<1..1266)
                     /gene="yiaN"
                     /locus_tag="KJL99_004592"
     CDS             complement(<1..1266)
                     /gene="yiaN"
                     /locus_tag="KJL99_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate transporter large permease
                     YiaN"
                     /protein_id="HBD4439020.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLLKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGIMMGATLMLTWWRQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLVVFVFIPDLIILPLK"
     gene            complement(1269..>1581)
                     /locus_tag="KJL99_004593"
     CDS             complement(1269..>1581)
                     /locus_tag="KJL99_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HBD4439021.1"
                     /translation="VAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWG
                     ATLKTIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQG
                     A"
BASE COUNT          482 a          400 c          406 g          293 t
ORIGIN      
        1 tttcaaaggg aggatgatga gatcgggaat aaaaacaaat acgactaata atgaatataa
       61 aaccaggacg taagggaaaa cgcctctgac tgcatcatcg aatttgagtt ttgccacccc
      121 tgaaataacg ttaagtacat taccgatagg cggtgtaatt aagccgattg agcagttaat
      181 gataaacatg acaccgaaat aaatcggatc gattcctgcc tctttaacta aaggcattaa
      241 taccggggta agaattaata ccgtcggcgt taaatccatg accatgccga caatcaggat
      301 agccaccata atgacgataa acagcagacg cggtgaatcg accaacggtt gcagtaaatc
      361 agaaaccatc atcggcagtt cagcaatggt aatcagccag gcggaaactt gtgccgaggc
      421 caccagaaac ataaccactg aggtggtttt cgccgcgcca atgagtacat gccaaagtgt
      481 ggcgaaggtc atttcacggt aaataactgt ggcgacaaac agcgcataga aagcagcaac
      541 ggccccggct tcggtagggg taaacagacc ggagcgaaac ccgccaataa tgatgactgg
      601 aagaaacagc gcccagatac cggagacaaa agagtgccag atttcctgca tcgttgcttt
      661 ttgctggcgg ggtaaattaa ggcgactggc ctgccgccac caggtaagca tcagcgttgc
      721 gcccatcatt atgccagggg caatgcccgc cataaacagc ttactgatgg ataatccgct
      781 ggaaacaccg aagataataa acggaatgga agggggaata attggcgcga taatgccgcc
      841 agaggcaatc agccccgccg cccggttgac cgggtagttg gcgctgcgca tcatcggcac
      901 cagtagagcg gcaacggcgg cggtatccgc aacggcagaa ccagaaaggc tcgccataat
      961 catagctgcc agcacgccga cgtagcccag cccgcctggt ttatgcccca ccagtttcat
     1021 cggcaggtca acaatgcgtt ttaacaagcc gcccgcattc atgatttcac ccgccagcac
     1081 aaagaacgga atcgccagga gggagaagct atcggctccg ttcaccagcg tttgtgccat
     1141 gatctggaca tcaaacatgt ccagccagaa cattaacgcc gccccgcaca acaacagtgc
     1201 ccaggcaata ggcaaaccga tagcaatacc acccaacaga cagcccagaa aaatcagcac
     1261 agccatgatt aagctccttg cggtggcgac gttagagaat tgctacgcgt gatgagttga
     1321 tataaatgac gcagttcaaa gaatgcgata acgaggctgg tgggcagaca agcggcatac
     1381 atcaggccga tgggcaaacc gaggatcggt gaataatcgc tccagtcctg aattgttttt
     1441 agcgttgcgc cccaggccag tgcgccacaa ataaataaga ttaaggaatg ggtaaccaga
     1501 gcgactcgtc gttgccatgc gggggagagt ttctccacca gaaaggtgac ctgaacgtgg
     1561 gcgttatcca taaaagccac a
//
LOCUS       DAEBXN010000326         1565 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.331, whole genome shotgun sequence.
ACCESSION   DAEBXN010000326 DAEBXN010000000
VERSION     DAEBXN010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.331"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            41..>1565
                     /locus_tag="KJL99_004594"
     CDS             41..>1565
                     /locus_tag="KJL99_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HBD4439022.1"
                     /translation="MDTSLAHENARLRALLQTQQDTIRQMAEYNRLLSLRVAAYASEI
                     NRLKALVAKLQRMQFGKSSEKLRAKTERQIQEAQERISALQEEMAETLGEQYDPVLPS
                     ALRQSSARKLLPASLPRETRVIRPEEECCPACGGELSSLGCDVSEQLELISSAFKVIE
                     TQRPKLACCRCDHIVQAPVPSKPIARSYAGAGLLAHVVTGKYADHLPLYRQSEIYRRQ
                     GVELSRATLGRWTGAVAELLEPLYDVLRQYVLMPGKVHADDIPVPVQEPGSGKTRTAR
                     LWVYVRDDRNAGSQMPAAVWFAYSPDRKGIHPQNHLAGYSGVLQADAYGGYRALYESG
                     RITEAACMAHARRKIHDVHARAPTDITTEALQRIGELYAIEAEVRGCSAEQRLAARKA
                     RAAPLMQSLYDWIQTQMKTLSRHSDTAKAFAYLLKQWEALNVYCSNGWVEIDNNIAEN
                     ALRGVAVGRKNWLFAGSDSGGEHAAVLYSLIGTCRLNNVEPEKWLRYVIEHIQDWPAN
                     "
BASE COUNT          380 a          432 c          457 g          296 t
ORIGIN      
        1 cgcgctggaa tccgcatata acccgttgta aagtgaggat atggacacct cacttgctca
       61 tgagaatgcc cgcctgcggg cactgttgca gacgcaacag gacaccatcc gccagatggc
      121 cgaatataac cgcctgctct cactgcgggt ggcggcttat gcttccgaaa tcaaccggct
      181 gaaggcgctg gttgcaaaac tgcagcgcat gcagttcggt aaaagctcag aaaaacttcg
      241 cgcaaaaacc gaacgacaga tacaggaagc acaggagcga atcagcgcac ttcaggaaga
      301 aatggcggaa acgctgggtg agcaatatga cccggtactg ccatccgccc tgcgccagtc
      361 ttcagcccgt aaactgttac cggcctcact tccccgtgaa acccgggtca tccggccgga
      421 agaggaatgc tgtcctgcct gtggtggtga actcagttct ctgggatgtg atgtgtcaga
      481 gcaactggag cttatcagca gcgcctttaa ggttatcgaa acacaacgtc cgaaactggc
      541 ctgttgccgg tgcgaccata tcgtgcaggc accagtacct tcaaaaccca ttgcacgcag
      601 ttatgccgga gcggggcttc ttgcccatgt tgtcaccggg aaatatgcag accatctgcc
      661 gttataccgc cagtcagaaa tataccgtcg tcagggcgtg gagctgagcc gtgccacact
      721 ggggcgctgg acaggtgctg ttgctgaact gctggagcca ctgtatgacg tcctgcgcca
      781 gtatgtgctg atgcccggta aagtccatgc cgatgatatc cccgtcccgg tccaggagcc
      841 gggcagcggt aaaacccgga cagcccggct gtgggtctac gtccgtgatg accgtaacgc
      901 cggttcacag atgcctgcgg cggtctggtt cgcgtacagc ccggaccgga aaggcatcca
      961 tccacaaaat cacctggccg gttacagcgg agtgcttcag gccgatgctt acggtggcta
     1021 ccgggcgtta tacgaatccg gcagaataac ggaagccgcg tgtatggccc atgcccggag
     1081 aaaaatccac gatgtgcatg caagagcgcc aaccgatatc accacggaag ccctgcagcg
     1141 tatcggtgaa ctgtatgcca tcgaggcaga agtccgggga tgttcagcag aacagcgtct
     1201 ggcagcaaga aaagccagag ctgcgccact gatgcagtca ctgtatgact ggatacagac
     1261 tcagatgaaa acactgtcgc gtcactcgga tacggcaaaa gcgttcgcat acctgctgaa
     1321 acagtgggag gccctgaacg tgtactgcag taatggctgg gtggaaatcg acaacaacat
     1381 cgcagagaac gccttacggg gagtggccgt aggccggaaa aactggctgt tcgcgggttc
     1441 cgacagcggt ggtgaacatg cggcggtgtt gtactcgctg atcggcacat gccgtctgaa
     1501 caatgtggag ccagaaaagt ggctgcgtta cgtcattgaa catatccagg actggccggc
     1561 aaacc
//
LOCUS       DAEBXN010000327         1561 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.332, whole genome shotgun sequence.
ACCESSION   DAEBXN010000327 DAEBXN010000000
VERSION     DAEBXN010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.332"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            400..1149
                     /locus_tag="KJL99_004595"
     CDS             400..1149
                     /locus_tag="KJL99_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBD4439023.1"
                     /translation="MKRQLFTLSIVGVFSLNTFASFPPGNDVTTKPDLYYLTNDNAID
                     SLALLPPPPQIGSIAFLNDQAMYEKGRLLRNTERGKLAAEDANLSSGGVANVFSAAFG
                     SPITAKDSPELHKLLTNMIEDAGDLATRSAKEYYMRIRPFAFYGVSTCNTKEQDTLSR
                     NGSYPSGHTSIGWATALVLSEINPARQDTILKRGYELGDSRVICGYHWQSDVDAARIV
                     GSAIVATLHSNPVFQAQLQKAKDEFANNQKK"
     gene            complement(1319..>1561)
                     /locus_tag="KJL99_004596"
     CDS             complement(1319..>1561)
                     /locus_tag="KJL99_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBD4439024.1"
                     /translation="ELKNNGIKAVIPRKSNEKMASDGRAQLDRDAYCNRNVVERCFGR
                     LKEYRRIATRYDKTARNYLAIVKLGCIRLFYQRLRN"
BASE COUNT          427 a          332 c          315 g          487 t
ORIGIN      
        1 ccataaacag ggctttaccc gtaaatattg gctccttctg atgccattct atttcaggaa
       61 ggagtgtccg ttaaactcag gctacctcaa aaagttatcg gtgcgtttat cgaaaaacat
      121 atgttctact aattggagtc attgtctgta aaagcaactc aaaaatacaa gttgcttatt
      181 ccccttgttg gtaagaactt aattctaact gtttgttctg ggtgttttaa acgtcctcct
      241 ttttatgctt gttcataacg gatatttaat aggtaacctt tgttttagca tcttctgatt
      301 tatttaaatt atgtgatagt acacacaatt ttattatcca gtactttatg ctcttaataa
      361 aataattata aatatgtatc ctctataagg agatgttata tgaaaagaca gctttttact
      421 cttagtattg tcggggtatt ttctttaaat acctttgcat cattccctcc gggaaatgat
      481 gtgacaacaa aacctgacct ttactacctg acaaacgata atgctattga cagtctggca
      541 ttattaccgc ctccaccaca aatcggaagt attgccttcc tgaatgacca ggccatgtat
      601 gaaaaggggc gtttattgcg aaatactgaa agagggaagc tggcagctga ggatgccaat
      661 ctcagtagtg gtggcgtggc aaatgtattt tctgctgctt ttggttctcc gataacggct
      721 aaagattcac cagaattgca taaattactg acaaatatga ttgaggatgc cggagaccta
      781 gcaacgcgtt cagcaaaaga atattatatg cgtattcgac cttttgcttt ttatggtgtt
      841 tctacgtgta atacaaaaga acaggataca ttatccagaa atggctctta tccatcaggt
      901 catacatcga ttggttgggc aacagcactt gtattgtccg aaataaatcc ggcacgccag
      961 gataccattc tgaaacgggg ttatgaactg ggggacagca gggttatttg cggttatcac
     1021 tggcaaagtg atgttgacgc agcacgtatt gttggctctg ctattgtagc aacactgcac
     1081 tcaaatcctg tgttccaggc acaattacaa aaagcgaaag atgaattcgc taacaatcag
     1141 aaaaaataat gcttttagcc agtacgcctt ataaccccga ccttcagggc taatgccagt
     1201 cagttaagca actgactggc tctttttcag ggctgtgggg tatttccagt gcctctcctt
     1261 taccactctc ggaaaggccc tttcccttct tgtcggtaat ttcactaggc tgtgtccctt
     1321 aattacgtaa gcgttgataa aagagtcgga tgcagcccag tttcactatc gccaggtaat
     1381 ttctcgccgt tttgtcgtaa cgcgtggcga tgcggcgata ttctttcagc cgcccaaagc
     1441 accgttcaac gacattacga ttgcagtacg catcacgatc aagctgtgca cgtccatccg
     1501 atgccatttt ctcatttgac tttcggggga ttactgcctt tataccgtta tttttcagct
     1561 c
//
LOCUS       DAEBXN010000328         1561 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.333, whole genome shotgun sequence.
ACCESSION   DAEBXN010000328 DAEBXN010000000
VERSION     DAEBXN010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.333"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            459..>1561
                     /locus_tag="KJL99_004597"
     CDS             459..>1561
                     /locus_tag="KJL99_004597"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HBD4439025.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKLGPYEFICT"
BASE COUNT          422 a          373 c          360 g          406 t
ORIGIN      
        1 tgttgaaaca aatgacggta gagaatgtta acactgccgt taaacaatat ctttctcatc
       61 cggtaaatac ttataccgga gtattattgc caaaataata acagccccgt caacacatcg
      121 ttggcgggga ttttagcaat attcgctatt tttatgtaat aattttataa atgcgttcaa
      181 aataataacc aagtactaat agtgatattt taatacctga tttttacgtg ataattcagg
      241 aggcacagaa agcgcataaa aataacaaca taaaacacct tgccaccacc caagaattct
      301 ctattacatt gtttttaatg aaaaaatatc attccaacaa tatctcacga taaataacat
      361 taggattttg ttatttaaac acgagtcctt tgcacttgct tactttatcg ataaatccta
      421 cttttttaat gcgatccaat cattttaagg agtttaaaat ggataagaag caagtaacgg
      481 atttaaggtc ggaactactc gattcacgtt ttggtgcgaa gtctatttcc actatcgcag
      541 aatcaaaacg ttttccgctg cacgaaatgc gcgacgatgt cgcatttcag attatcaatg
      601 atgaattata tcttgatggc aacgctcgtc agaacctggc cactttctgc cagacctggg
      661 acgacgaaaa cgtccacaag ttgatggatt tatccattaa caaaaactgg atcgacaaag
      721 aagaatatcc gcaatccgca gccatcgacc tgcgttgcgt aaacatggtt gccgatctgt
      781 ggcatgcgcc tgcgccgaaa aatggtcagg ccgttggcac caacaccatt ggttcttccg
      841 aggcctgtat gctcggcggg atggcgatga aatggcgttg gcgcaagcgt atggaagctg
      901 caggcaaacc aacggataaa ccaaacctgg tgtgcggtcc ggtgcaaatc tgctggcata
      961 aattcgcccg ctactgggat gtggagctgc gtgagatccc tatgcgcccc ggtcagttgt
     1021 ttatggaccc gaaacgcatg attgaagcct gcgacgaaaa taccatcggc gtggtgccga
     1081 ctttcggcgt gacctacacc ggtaactatg agttcccgca accgctgcac gatgcactgg
     1141 ataaattcca ggccgacacc ggtatcgaca tcgacatgca catcgacgcc gccagcggtg
     1201 gctttctggc accgttcgtc gccccggata tcgtctggga cttccgcctg ccgcgtgtga
     1261 aatcgatcag tgcttcaggc cataaattcg gtctggctcc gctgggctgc ggctgggtta
     1321 tctggcgtga cgaagaagcg ctgccgcagg aactggtgtt caacgttgac tacctcggtg
     1381 gtcagattgg tacttttgcc atcaacttct cccgcccggc gggtcaggtg attgcacagt
     1441 actatgaatt cctgcgcctt ggtcgtgaag gctataccaa agtacagaat gcttcctacc
     1501 aggtcgctgc ctatctggcg gatgagatcg ccaaactggg gccgtatgag ttcatctgta
     1561 c
//
LOCUS       DAEBXN010000329         1510 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.335, whole genome shotgun sequence.
ACCESSION   DAEBXN010000329 DAEBXN010000000
VERSION     DAEBXN010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.335"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(157..639)
                     /locus_tag="KJL99_004598"
     CDS             complement(157..639)
                     /locus_tag="KJL99_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBD4439026.1"
                     /translation="MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNAS
                     RTFVICLEDTLRIVGYYSLATGSVTHAELGRSLRHNMPNPVPVVLLGRLAVDVCTQGH
                     GFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV
                     "
     gene            complement(630..932)
                     /locus_tag="KJL99_004599"
     CDS             complement(630..932)
                     /locus_tag="KJL99_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HBD4439027.1"
                     /translation="MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRT
                     DFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLEAPVQNAEGRERLMAVKPEWK"
     gene            1204..1476
                     /locus_tag="KJL99_004600"
     CDS             1204..1476
                     /locus_tag="KJL99_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4439028.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYKSVFADFITGHPSC
                     TVCFWETFHKMYPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
BASE COUNT          429 a          378 c          322 g          381 t
ORIGIN      
        1 ccttcactga tcgaaagctg attttcagga accgttttga cggcttcggc tttaaactcc
       61 gtagagtaac gttgggtttt tctgctcatt attggctcct tctgatgcca ttctatttca
      121 ggaaggagtg tccgttaaac tcaggctacc tcagactcag actttataaa acaaggtatt
      181 aggtgccaca actgactgaa caaaaccaaa acgctcataa aatgctctgg cgtcatcatc
      241 aattgcatgt accataacag ccttaatgcc aacctgatca gccagattga caacccggtg
      301 aatggcatca cttagtagcc atttaccgaa accatgcccc tgggtacaaa catcaacagc
      361 cagacgccct aacagaacaa cagggacagg attgggcatg ttatgccgca aactgcgacc
      421 aagctcggca tgagtgacgg aaccagtagc taatgagtag tatcccacaa tacgtaaagt
      481 atcctccaag caaataacga acgtgcgaga agcattgagc atctggtttt tcatagcccg
      541 accacgtaac cagttactta gcacctcatt tccacagtca aatggctgaa gtatatgctc
      601 atccgtcaac aatgctggcg cggtgacatt tatttccatt caggctttac cgccatcaga
      661 cgttcacgcc cttctgcatt ctgcacagga gcttccagtt gcgtaataaa cgaatcataa
      721 cgttcgttat ccagcacaaa taaacgttga tccaggataa tgtttttagc ttcctggtac
      781 gccagttcca tgatgaaatc ggttcggttc ttattgacca agctcgcagc ataatcaata
      841 acggctcgtt cctcatcagt tgcccggatg ttaatctgat gttctcgcgg tgttttacgg
      901 acgcttgcag ctgtagacat aaaaacctcc attggtaaca atttgctatg caattaatgt
      961 tatcgcaaat ggtaacagaa cgctatacag aatcacttac gggaaaagcg atcccctgat
     1021 aaaaacaatg gcagagacct gaacattgtg acgatgatct gtgtcggcgc acaaatcatg
     1081 tctgattacg gccgtttaat gtgattttga taatgaaatg cctgaaccac ggcagcctgg
     1141 cggctgcaaa tcggggtgtg gcagaatatg agctttcaga gtaaccatcc agaatgagtt
     1201 cccatgtcac acaacctgga gcaccagaaa gtccacacca gaatggtgaa agaggttctg
     1261 aaagccgtcg ccagagccaa taatcaccct tataagtccg tatttgcaga ctttatcacc
     1321 ggtcatccct cctgcacggt ctgtttctgg gaaaccttcc acaaaatgta cccggattca
     1381 ccgtacgaat acgtcacgtt ctgccatacc tgccgtcgtt ttgatttata cgaaacagaa
     1441 gcggagatga aggcggatga cccgaagtgg tggtgatttt cagccccgac cgctgaaacg
     1501 cctgttcaca
//
LOCUS       DAEBXN010000330         1470 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.337, whole genome shotgun sequence.
ACCESSION   DAEBXN010000330 DAEBXN010000000
VERSION     DAEBXN010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.337"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(5..184)
                     /locus_tag="KJL99_004601"
                     /pseudo
     CDS             complement(5..184)
                     /locus_tag="KJL99_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051932.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            708..992
                     /locus_tag="KJL99_004602"
     CDS             708..992
                     /locus_tag="KJL99_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HBD4439029.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQSWADSLSTDHLLPVPR"
     gene            992..1267
                     /locus_tag="KJL99_004603"
     CDS             992..1267
                     /locus_tag="KJL99_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBD4439030.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIYVLRLWHTRENR"
BASE COUNT          401 a          368 c          361 g          340 t
ORIGIN      
        1 aaaattactc tttttcgaac tccagtgagc gttcacgact catcagtgtg aaaatgatgg
       61 caaaaacagc agacagtatc agtacgataa atgtggcccc ccatcccaga taatcgacaa
      121 caagtcccat tcccagtgtc gcaacaattg agccgaagat ataggaaaat gtgcctgaca
      181 gccctaacag aataagttta cgcgtaaatc gccctgaatc cagaagagca ttaagtatca
      241 cataaacagg ttccatacca gtcctgaccg catcaggacg ccagtaaata aaatctcgta
      301 gtccaccggc atcagagaca agagtaatcg tcgttaccgg tatttcgact ggtttgtact
      361 ggtgtgagcg gacaaaacta ctgtacgtct ctcatacacg ctcgtaagcg gccctcacgg
      421 cccgctaacg cggagatacg ccccgactgc gggtaaaccc ttgtcgggac cactccgacc
      481 gcgcacagag gcattttcat cgctgacagg ccgcatggcc tcgcagagcc ggagggggac
      541 tggtgatatt cggacgaccg taccggcttg cgccgggctt cggcggtttt acacctgtgc
      601 tacatgtaac caccgtatgc cgccttatat gcgctgactc gcattatttg ccttgttccg
      661 tctgaaagta atcactatga ttaaatatga ttaacaacta atcgggtatg caaatgaaaa
      721 acaataccgc acaagcaaca aaagtaatta ccgcgcatgt gccattacct atggctgata
      781 aagtcgacca gatggccgcc agactggaac gctcccgggg ctgggttatc aaacaggcgc
      841 tttctgcatg gcttgcccaa gaggaggagc gtaatcgcct gacgctggaa gccctggacg
      901 atgtgacatc cggacaggtt atcgaccatc aggctgtaca gtcctgggcg gacagcctca
      961 gtactgacca tctgttaccg gtgccacgct gatggaactg aagtggacca gtaaggcact
     1021 ttctgatttg gcgcggttat atgattttct ggtgctggcc agtaaacctg ctgccgccag
     1081 aacggtgcag tccctgacac aggcgccggt cattctgtta actcatccac gtatgggaga
     1141 acagttgttt cagtttgaac ccagggaggt cagacggatt tttactggcg agtacgaaat
     1201 ccgttacgaa cttactggcc agactattta tgtattgcgc ctgtggcaca cacgagagaa
     1261 caggtagatt gggtgtggag gcaactttaa catagcctgc aggtattaat tctggagcat
     1321 tgttatcagg ccagatattt tgcattccat taaataatct tgtaggagat aaaataaatt
     1381 acgatgaaat aacgaaaatt accgctcgtg actggcggca atatcgcgct ccgggctggc
     1441 aaataaccca tcagaaacgt tattgccaga
//
LOCUS       DAEBXN010000331         1436 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.338, whole genome shotgun sequence.
ACCESSION   DAEBXN010000331 DAEBXN010000000
VERSION     DAEBXN010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1436
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.338"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            41..>1436
                     /locus_tag="KJL99_004604"
     CDS             41..>1436
                     /locus_tag="KJL99_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HBD4439031.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHTLNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
BASE COUNT          385 a          302 c          394 g          355 t
ORIGIN      
        1 tttctgggtc acaataactc accaccgtga gggaagaata atgaaaatgg tctcacgtat
       61 taccgcgatc ggcctggctg gcgtcgcgat ttgctattta gggttatctg gttatgtgtg
      121 gtaccacgat aataaacgca gtaaacaggc cgatgttcag gcatctgctg tcagtgaaaa
      181 taataaggtt ttaggctttc tccgcgaaaa aggatgcgac tattgccaca cgccttcggc
      241 agaattaccc gcctattatt atattcctgg cgcgaaacag ttgatggatt acgacattaa
      301 gcttggatat aaatctttta acctcgaggc cgtgcgtgcg gcactgctgg ctgataaacc
      361 cgtttcgcaa agcgatctga ataagattga atgggtgatg cagtatgaaa ctatgccacc
      421 aacgcgttat accgcgctac actgggcggg taaggtgagt gatgaagagc gggcggaaat
      481 actggcctgg attgcaaaac agcgcgcgga atattacgcc agcaatgata ctgctccgga
      541 gcatcgcaat gaaccggtgc agcccatccc gcaaaaactg cctaccgatg cgcaaaaagt
      601 ggcgttgggc tttgcgctgt atcacgatcc ccgtttatcg gctgatagca ccatttcatg
      661 cgctcattgc catacgttga atgcgggggg cgtcgatggc agaaaaacat cgattggtgt
      721 tggtggcgca gttgggccga ttaatgcgcc gacggtattt aactcagtat ttaacgttga
      781 gcagttctgg gatggtcgtg cggcaacatt gcaggatcag gcaggtggac cgccgttgaa
      841 cccgattgaa atggcgtcga aatcctggga cgaaattatt gctaagctgg aaaaagatcc
      901 gcagcttaaa gcgcagttcc tcgaagttta tccgcaaggt ttcagtggcg aaaatattac
      961 tgatgccatt gctgaatttg agaaaacatt aattacgccg gattccccat ttgataaatg
     1021 gttgcgtgga gatgaaaatg cgctgacggc gcaacagaaa aaaggctatc aattatttaa
     1081 agataataaa tgtgcaactt gtcatggtgg tattattctc ggcggacgtt cctttgaacc
     1141 gttggggctg aaaaaagact ttaactttgg ggaaattacg gcggcggata ttggtcgtat
     1201 gaatgtgact aaagaagagc gtgataaatt gcgtcagaaa gtacccggtt tacgtaacgt
     1261 tgctttaacg gcaccgtact tccatcgcgg tgacgtgccg acgctggacg gggcggtgaa
     1321 actgatgctg cgctatcagg taggcaaaga gctgccgcag gaggatgtgg atgatatcgt
     1381 agctttcctg cacagtctga acggggtgta cacgccgtat atgcaggata aacaat
//
LOCUS       DAEBXN010000332         1404 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.339, whole genome shotgun sequence.
ACCESSION   DAEBXN010000332 DAEBXN010000000
VERSION     DAEBXN010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.339"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..167
                     /locus_tag="KJL99_004605"
     CDS             <1..167
                     /locus_tag="KJL99_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HBD4439032.1"
                     /translation="AIASTPHPSWRRICKTLIKNDFWCRTLSFSPNKPRHYERYLQRM
                     KERRKEWGTL"
     gene            152..790
                     /locus_tag="KJL99_004606"
     CDS             152..790
                     /locus_tag="KJL99_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HBD4439033.1"
                     /translation="MGDSVTPEILGNMIRQYFSQVRTEKESIQALNHLRRVLHEVSPF
                     AQEPVDCVLWVKADEVVANDYNPNVMALGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQLLGREADAGKRLRGWLPVACINPERKGQAARIAATIRHNRARGKHQITLMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSSAWTVR"
     gene            1018..>1404
                     /locus_tag="KJL99_004607"
     CDS             1018..>1404
                     /locus_tag="KJL99_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4439034.1"
                     /translation="MKKQIFINNKPPVVPYSGTHAKIFKYIEIPLPFFYFIYTSGEPF
                     HISVQNTVIYVSKYNGIFINKLVPFSLLFDRDISVLQRRDICVVRFTSEEISEHNVLF
                     DHDIERLKKISKAQLISPDYVLIDFSS"
BASE COUNT          407 a          257 c          347 g          393 t
ORIGIN      
        1 ccgctattgc cagtacacct cacccgtcct ggcgacgtat ctgtaagaca ctcataaaga
       61 atgatttctg gtgccggact ctctccttca gtccgaacaa accccggcac tatgaacgtt
      121 acctgcagcg tatgaaggaa aggaggaagg aatgggggac tctgtgacac cggagatact
      181 tggcaacatg attcgtcagt atttcagtca ggtacgaact gaaaaagaga gcatacaggc
      241 tctgaatcat ctgcgcagag tgcttcatga ggtcagtcct tttgcacagg agcctgtgga
      301 ctgtgtgttg tgggtgaaag cggatgaggt ggtggccaac gattacaacc ctaatgtcat
      361 ggcactaggt gagaaaaaac tactgaaaca gtctctggag aaagatggtt tcacacaacc
      421 ggtggtggtg tcagaggaga agaatcatta tctggtggtg gatggttttc atcgtcagtt
      481 actggggcgg gaagcagatg caggaaaacg cctgaggggc tggttgcctg tagcctgcat
      541 taatccggag agaaagggac aggccgcacg tattgccgcg accattcgac ataaccgggc
      601 cagggggaaa caccagatca ctttaatgtc tgatattgtc agggatcttt cacgtctggg
      661 ctggacgaat gaacgtattg gtacagagct gggaatggac caggatgaag tgctgaggct
      721 gaaacagatt agcgggctga cagagttgtt tcaggaagag gatttcagct cggcttggac
      781 ggttcgttga tgttgttaaa tatgggcggt atttctgaaa gttgatactt tgggtagaaa
      841 aaatgatacc gaaaactatg gaggatgtgc ttgtcaggtg actggacacc ataggtctta
      901 ggctttgata acgggataag atgagcggct tttctcatac catcaggtgt gatgatggcg
      961 cagatttaca aaatctgatt gattctaaag atgtataagt aaaaatcaag ctttgatatg
     1021 aaaaaacaga tatttattaa taataaacct cctgtagtcc cttactcagg gacgcacgcg
     1081 aaaatattta aatacattga aatccctctg ccgtttttct actttatcta tacatcaggc
     1141 gaaccattcc acatatctgt ccaaaatact gttatttatg tatcaaaata caatggtata
     1201 tttattaata aattagttcc tttttctctt ttatttgaca gggatatatc tgttcttcaa
     1261 agaagagata tttgtgttgt gcgatttacg tcagaggaaa taagtgaaca taacgtttta
     1321 tttgatcatg acattgaacg attaaaaaaa atttccaaag ctcagcttat atctcccgat
     1381 tatgttttga ttgatttttc tagt
//
LOCUS       DAEBXN010000333         1388 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.340, whole genome shotgun sequence.
ACCESSION   DAEBXN010000333 DAEBXN010000000
VERSION     DAEBXN010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1388
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.340"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(1..528)
                     /locus_tag="KJL99_004608"
                     /pseudo
     CDS             complement(1..528)
                     /locus_tag="KJL99_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(579..1154)
                     /locus_tag="KJL99_004609"
                     /pseudo
     CDS             complement(579..1154)
                     /locus_tag="KJL99_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1158..1388
                     /locus_tag="KJL99_004610"
                     /pseudo
     CDS             1158..1388
                     /locus_tag="KJL99_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          303 a          399 c          345 g          341 t
ORIGIN      
        1 tgctgaacat ccccggactt ctgcctcgat ggtatacagt tcaccgatac gctgcagggc
       61 ttccgtggtg atgtcggttg gcactcttgc atgcacatcg tggatttttc tccgggcatg
      121 ggccatacac gcagcttccg ttattctgcc ggattcgtat aacgcccggt aaccaccgta
      181 agcatctgcc tgaagcacac cgctgtaacc ggctaggtga ttctgtggat ggataccttt
      241 ccggtccggg ctgtacgcga accagaccgc cgggggcatc tgtgaaccgg cgttacggtc
      301 atcacggaca tagacccaca gccgggcagt tcttgtttta ccactgcccg gctcctggac
      361 cggaacgggg atatcatccg catgaacttt accgggcatc agtacatact ggcgcaggac
      421 gtcatacagc ggctccagca gttcagcaac ggcaccggtc cagcgaccca gtgtggcacg
      481 gctcagctcc acgccctgac gacggtatat ttctgactgg cggtataact gtcaacgacg
      541 gatgaaaagt ggctcttccg caacctgggt ggagattttc atgccagtgg actcattacc
      601 ctctgcctga gtagttcaaa tcctgctcga ccatacatct ggcgtttcag catcttcttc
      661 aggcgattta catgaccttc gacaacacca ttactccagc gactactgat tgcctcacat
      721 atagctgcag catcagcttc catccccgcc gccacgcgcc gaaattctgc tgaaccgctt
      781 tcgtggactc gagtgaacca actgcttagc tgtgatttat tttgggtttt cagaatacgg
      841 tagaactcga gtgccagttg ctgcgctatt ttcagctcag gttctttttc acacatcaga
      901 ctaataaatc gggaagcata attttcttca tccctggtta ttctccaggg catcaaccag
      961 cggcttaccc gggagactga aggaagtctg acggcagtag taaccggtgg gttccagcct
     1021 ttacgccatt tggcaaccgc atccctgacg atggtttcac tgcctgtaaa tccccgggcc
     1081 accatttccc gccatatccg gctggcgtta taattaccac tttctcgttg ctcttttaac
     1141 cactccctcc atggtgtcag cgaaaaacga aaccgctgtt gaaatccctg gaaagctggt
     1201 tgcggaaaag atgaagaccc agtcgcgaca ctcagagttg gcgaaggcgt tcgcgtacgc
     1261 acttaaccag tggccggcac tgacgtacta tgcgaacgat ggctgggtgg aaatcgacaa
     1321 caacatcgct gaaaatgcct tgcgggcggt cagtctgggt cgtaaaaact tcctgttctt
     1381 cggctctg
//
LOCUS       DAEBXN010000334         1356 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.341, whole genome shotgun sequence.
ACCESSION   DAEBXN010000334 DAEBXN010000000
VERSION     DAEBXN010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.341"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            83..449
                     /locus_tag="KJL99_004611"
                     /pseudo
     CDS             83..449
                     /locus_tag="KJL99_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            377..850
                     /locus_tag="KJL99_004612"
                     /pseudo
     CDS             377..850
                     /locus_tag="KJL99_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708890.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase
                     domain-containing protein"
BASE COUNT          326 a          342 c          407 g          281 t
ORIGIN      
        1 cttgccggtg ttctccgggg taaaaaggtc cacactaccg tcagccggaa agccgttgcc
       61 gcaggcgacc gcgtaaaccg tcatcaaggc aatgtgccgc gtactccggg tggcccgcag
      121 cggctggtat acgtggtgtc agcggcggac aaggataaac acacgtccgc agttccgtca
      181 gcactgcgac agcgttgtcc gcaaggcttt tacccagtcc agcgttacgg tgccccacgc
      241 ctgacggatg aactgtgtgc tctggttacc actttaacgt aaaaaccgtg gcagccagtc
      301 tgcgccgtca ggaactgagc gcaaaagcct cccagaagtt cagcccgatc agttaccgtg
      361 cacacggcct gcctgtatca gaaaatctgc tgacgcagga tttttacgcc agtggcccga
      421 accagaagtg ggcgggagac atcacgtact attacagctc accgactgcg gggaaacacg
      481 gggcgccggg ttattgaagg tgacctgtcc agctacttcg acacagtaca tcaccggctg
      541 ctgatgaaag ccgtctgccg caggatcagt gatacacgtt ttatgagact gctgtggaaa
      601 acgccgtgtt gatgttggtc tcttccgggc ggtcagtgaa agtatgccac agggcggtgt
      661 gatctcgccg ttgctgtcga acatcatact gaatgagttc gaccagtatc tgaataaacg
      721 ctacctgagt ggaaaagcca gaaaggatcg ctggtactgg aatcacagta tccagcgagg
      781 ccgaagtact gctgtcaaag aaaactggca atggaaacct gccgttgctt actgttgcta
      841 tgccgatgac tgtgtgccac gaaggcgcgt cctggggacg tgagtccctg tgcagccatg
      901 cagcacaaga gatgagggaa ggccccttgg tgtcggcgtg caggcgcagg caccaaacca
      961 tccactgctg ctgcatttaa gagatgtggt ggtgagcgag atgggaaaag gtcggacgtg
     1021 agccgatcag gtatggttcg aagagacgaa cgaaagtgaa ccgctgatga tgtgtcgaaa
     1081 gcatatagat gatgtcaaaa caatggtgga gtcgttcctg tgggatatgt ttactgcctg
     1141 cgcggcatcc ggcataaaag cggcgtgaag tcgattcagg ctcttgtggg gaacgtggac
     1201 ctgtcacccc gatgtgaagg gagaagcgca agtggaagcc ccatgagcgc cagagtacca
     1261 atgcggggta cagggacgga gcaccaagcg attgcgcagt aaacatgctt gacaacttaa
     1321 ttggggcccc ctcttccttt tatggtgtaa ttgttt
//
LOCUS       DAEBXN010000335         1338 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.342, whole genome shotgun sequence.
ACCESSION   DAEBXN010000335 DAEBXN010000000
VERSION     DAEBXN010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1338
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.342"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            1..253
                     /locus_tag="KJL99_004613"
                     /pseudo
     CDS             1..253
                     /locus_tag="KJL99_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899756.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            746..>1338
                     /locus_tag="KJL99_004614"
     CDS             746..>1338
                     /locus_tag="KJL99_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBD4439035.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYRAQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHESYDNGKSGEKFLQE
                     LCGVDLLVLDEIGMQRDTKNEQVVLNQIVDRRTASLRG"
BASE COUNT          325 a          312 c          411 g          290 t
ORIGIN      
        1 tgccaacaca aaggcaacta ataaagatac agataaagat ctaaaaacag atctaaccca
       61 tcccaaaccc ttcccttccg gaagggagtt tcgggatttt gtggctggag tgcttgaggg
      121 gagattatct ggcggtactg cagcggaatt ttgtaattct gcggtggttg cgttgcaggc
      181 tgctggcctg gatgtctgtc gtgagtatcc ggtgccagag cgtggtgacg gttgcggagg
      241 gcggattgat atcgtcgtga ctgacaggaa cggtgtccgg tgtgggatcg agcttgaccg
      301 aaattctccg cgacagaaat cactgctcaa aatcggtgct gttgaaaccg ggatatgtgt
      361 cttgcggcgc agtgatatcg caaggcacac cgagcaggga attctggtta tcggtggggc
      421 tgttcgccag aaaaaatttg acccgttgtc agttgatctg cccgactggt tgtcagaaac
      481 actctggcat gagtgggtcc agttcaggca ggcattgcga aaaccgattc gaacggagct
      541 gggcgctaac ggggcgatac gggaactgga aaaattccgt cagcagggtt ttacacctga
      601 gcaggtgatt cgacacagca tcgccaatga ataccagggt ctgttcgcgc cgaaaggtgt
      661 tcggcctgag acgttgctcc gacaggttaa caccgtctcg ttgccggaca gtgcgatccc
      721 gccaggcttc agggggtaac agaccatgaa aaatattgcg acaggaggcg ttctggagcg
      781 tatccgcaga ctgaccccac cacatgtaac cgccccattc agaacggttg cggagtggcg
      841 cgagtggcaa cttgctgaag gccagaaacg ttgcgaggag atcaaccgcc tgaatcgtca
      901 gttgcgggtg gaaaaaatcc tgaaccgctc cggcatccag ccgttgcacc ggaagtgttc
      961 gtttgcgaat taccgggcgc agaacgacgg tcagcgacat gcactgagtc aggcgaaatc
     1021 catagctgac gaactgatga caggctgtac gaattttgtg ttcagcggta agcccggcac
     1081 cggaaaaaat catcttgcag ccgcgattgg caaccggctg atggcgaagg ggcgtagcgt
     1141 gattatcgtc accgtgtccg atgtcatgag cgtgttgcat gagagctacg acaacggcaa
     1201 atccggtgaa aaatttttac aggagctttg tggtgttgac ctgctggtcc tggatgaaat
     1261 tggcatgcag cgggatacga aaaacgagca ggtggtactg aaccagattg ttgatcgccg
     1321 gacggcatcg ttacgcgg
//
LOCUS       DAEBXN010000336         1336 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.343, whole genome shotgun sequence.
ACCESSION   DAEBXN010000336 DAEBXN010000000
VERSION     DAEBXN010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.343"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(207..362)
                     /gene="hokD"
                     /locus_tag="KJL99_004615"
     CDS             complement(207..362)
                     /gene="hokD"
                     /locus_tag="KJL99_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HBD4439036.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESRK"
     gene            complement(590..1255)
                     /locus_tag="KJL99_004616"
     CDS             complement(590..1255)
                     /locus_tag="KJL99_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HBD4439037.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASEIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
BASE COUNT          331 a          342 c          314 g          349 t
ORIGIN      
        1 ccatccctct tctccctgcg cgctacttaa gtgagtcgat tctatctgcg aaggtgcgcg
       61 aatcaaattc accggaaata aacaacaaaa aacccgccga aacgctgaga gatcacagcg
      121 atgggcgggt taagtgcggg tgcgttgagg atgcctgaca catcagaggt gggcggggat
      181 gggatcagct ccccacccgg tcactcttac ttcctggatt cgtagtctac gaagacagcg
      241 acctccgtct ggccggttcg gattcgtacc tcacagaggt ctttcctcgt taccagtgcc
      301 gtcactatga cggttaaaca gatgacgatc agggcgatta acatcgcctt ttgctgcttc
      361 atagcctgct tctccttgcc tttcggcatg taagaggcta acctacatgt gcaaagcatg
      421 aaattggcct cagattaatg ttaagcgtct tgccggacgc gtaatgttaa ctggggcttt
      481 tctctatctg ccttttggtg ttcatgcctg aagcagatag cctcaagcac ccgcagtcat
      541 tctacttacc tcgccaatat gaaatcaatc agaaaggtgc cccataaaat cactccttct
      601 cttctttacc gtagtggagt tgaccaattt tgataagagg gcgtccctga gatttgcggt
      661 gtagattggt atcgcgcaga gaatacacac agccacaata ttcctgctga tagaattttt
      721 cgcgcttgct gatttcaatc atacgggacg agccgccctg cttgcgccag ttataatccc
      781 agtacaccat gcccggataa tgcgcgacgg ctcgccgccc acagtcgtta acctgctgca
      841 tatttttcca gcgtgaaatg cccagtgaac tgctgatcac actgaaacca ttttcagcgg
      901 cgtacagcgc tgtccgctca aaacgcatgt caaaacacat ggtgcaacgg atccctcgtt
      961 cgggctccca ttccattcct ttggctcgtt caaaccagtt gtctgtgtcg taatcagcat
     1021 caataaacgg cacgccgtgt tgttcagcaa agcgaatatt ctcatcctta cgaattaaat
     1081 actctttctg aggatgaatg ttcgggttgt agaaaaagat ggtgtagtcg atttccgagg
     1141 cctgaagcgc ctccatcacc tcaccggaac atggagcaca gcaagagtgc agtagtagtt
     1201 tgtttgcccc gtttgggagc tccaatttgg gccgtttgaa atcagcaact gtcataaata
     1261 tgtttattga ggtcataaaa atatcaaaga gtgtagcatt agaacggggc tatcggaaac
     1321 agatgtgtga ctgctc
//
LOCUS       DAEBXN010000337         1330 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.344, whole genome shotgun sequence.
ACCESSION   DAEBXN010000337 DAEBXN010000000
VERSION     DAEBXN010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.344"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(41..100)
                     /locus_tag="KJL99_004617"
     CDS             complement(41..100)
                     /locus_tag="KJL99_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HBD4439038.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            complement(339..1247)
                     /locus_tag="KJL99_004618"
     CDS             complement(339..1247)
                     /locus_tag="KJL99_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4439039.1"
                     /translation="MTLEQEQQVKTRTAEQNARIAAFEAERRREAEQTRILAERQIQE
                     TEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKSIAIAAKSEQQSQAEARANL
                     ALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETKAVELTVRAKAEKEAAEMQA
                     AAIVELAEATHKKGLAEAEAQRALNDAINVLSDEQTSLKFKLALLQALPAVIEKSVEP
                     MKSIDGIKIIQVDGLNSGSAAGDANTGNVGGGNLAEQALSAALSYRTQAPLIDSLLNE
                     IGVSGGSLAALTSPLISTTPVAENVK"
BASE COUNT          266 a          354 c          321 g          389 t
ORIGIN      
        1 acaagggtga gggaggattt ctcccccctc tgattggctg ttaataagct gcgaaactta
       61 cgagtaacaa cacaatcagt atgatgacga gcttcatcat aaccctttcc ttctgtaagg
      121 ccccttcctc gggaggggct ttcccgtttc agccctttgc tgactccccg attgttgatg
      181 agcgccggac ctcgcgcaac gctttcccag aggcagtttt gccttccccg agcggtcacg
      241 caaaacgttg caacgacctg atcatttgca gtttttagcg gaacgcagat ataaaaaatc
      301 cccggctttt tgtgccaggg acgttcatag ctgaaaattt attttacgtt ttcggcgacg
      361 ggagttgttg agattaaggg tgaagtcaat gccgccagtg aaccgcctga aacgccaatt
      421 tcattgagca aggagtcaat cagcggagcc tgtgtgcggt aagagagagc ggctgataat
      481 gcttgttccg ctaggtttcc gccccccaca ttacccgtgt ttgcatcacc cgcagcgctg
      541 ccactattca gaccatcgac ctgaataatc ttgataccgt caatagattt catcggctca
      601 acggattttt ctattaccgc aggtagcgcc tgcaacaagg ccagtttgaa tttaaggctg
      661 gtttgttcat cagaaagtac gttgatagca tcgttcagcg cacgttgtgc ttctgcttcc
      721 gccagaccct ttttatgtgt agcttcggct aactcaacga tagccgccgc ctgcatctct
      781 gcggcttctt tttctgcttt cgcccgcacg gtcagttcaa ccgctttggt ttctgcatcc
      841 tgcgcggcag cgattagggc aacttgttta gcacgatcgg cttctgcggt ctggcgagtg
      901 gtttctacgt tttgttgcgc acttactgct tctgcgagtg caaggttagc acgcgcttct
      961 gcctgggact gttgttccga tttggcggca atagcgatcg atttcgtctg gttggcgatt
     1021 tcggtgacct gctgctgttc gatctcttta atacgaactt cacgttcagc ctcaaccttt
     1081 cttgagcgga cggcctgctc gcgctcgatc tccgtttcct gaatttgtcg ttcagccaga
     1141 attcgcgtct gctctgcttc acgacgacgt tcagcttcaa aagccgcaat acgtgcattc
     1201 tgttcggcag tacgggtttt aacctgctgc tcctgctcaa gcgtcataaa cgcttcttgt
     1261 tgttcggtaa tgactccaac ttactgatag tgttttatgt tcagataatg cccgatgacc
     1321 ttgtcatgca
//
LOCUS       DAEBXN010000338         1279 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.345, whole genome shotgun sequence.
ACCESSION   DAEBXN010000338 DAEBXN010000000
VERSION     DAEBXN010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1279
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.345"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(<1..835)
                     /gene="ipaH4.5"
                     /locus_tag="KJL99_004619"
     CDS             complement(<1..835)
                     /gene="ipaH4.5"
                     /locus_tag="KJL99_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HBD4439040.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQSLKYLKVGENQLRRL
                     SRLPQELLALDVSNNLLTSLPENIITLPICTNVNISGNPLSTHVLQSLQRLTSSPDYH
                     GP"
BASE COUNT          358 a          230 c          287 g          404 t
ORIGIN      
        1 gcgggccgtg gtagtccggc gaagaggtta atctttgcag ggattgcaga acgtgagtcg
       61 acaatggatt ccctgaaatg ttaacattcg tacaaatggg caatgtgatt atattttcgg
      121 gtaatgaagt tagtaggttg ttggaaacat ccagtgccaa tagttcttgc ggtaatctgg
      181 ataatctgcg tagttgattt tcacctacct tcaaatattt taagctctga ggaaattctg
      241 gtagttcctg tagtctgttg ccggaaacaa agagttcttg tagtctatga ggcaattcag
      301 gcaagacagt aaggcgatta ccttcaatac gtaatattgt tagcgttgat ggaagctctg
      361 gtaacgtctc cagactatta aaacgtgccg agagtgattg taaatatggt ggtaatgatg
      421 gcagacatga caaatttcga ttgaatgaaa tattaagctt aatcagttga gatggaagtg
      481 tcgggagtat attcaagttg ttaccattta catgaagttc tgtcaaaagc ggagaatttt
      541 caggtaggga gattaactca ttgttggaaa tattaagttc acgtatatgc aaagggagtg
      601 gtggtaaaga acgtagtttc agtaagctta aattgagaac aggctcccgg gtatccagac
      661 atattttcaa taatcgaacc gcctggatgc gattttctgt tgttgttcct tccctagccc
      721 agtcatccca gatgcggtgg taatctctgg tacactgttc ttctaccgat aaacgagata
      781 tacatgataa gccacaaagg gaacgaaaaa aagaatgatt gttgatcggt ttcatttatt
      841 aacccctcac tattcatatc ttggtctatt aatgcaaata aaacgagaaa gtctttaaaa
      901 aaacaatcct agagctttat cggcgttttc attccggctg gaggaatatg ttcctgtctt
      961 tccagtaacc gtcgattgtt atatcagtct acccatgtta atgtgaccag ttctactttc
     1021 atccagtttg gattgatctt tatcccctat attttcaaac aatttttatc atttaactca
     1081 ttactgtctc gatattccca tagcaaacga tctggttggt tatcccctca aaaaagtcag
     1141 ttgcacgccg taaatccctg ccggtattga gcgaggattt acgggttgtt gttaaggtcc
     1201 gtaatcgggt cacagttttt ccggagtcaa tccggtctgc ggtttatgct tatgcgacgt
     1261 gattatgaat ggtgcagtc
//
LOCUS       DAEBXN010000339         1275 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.346, whole genome shotgun sequence.
ACCESSION   DAEBXN010000339 DAEBXN010000000
VERSION     DAEBXN010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.346"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            34..435
                     /locus_tag="KJL99_004620"
     CDS             34..435
                     /locus_tag="KJL99_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HBD4439041.1"
                     /translation="MAKKDDNLKRLRELAASLGREPDISGSAADIAQRVAELEEELAN
                     MDDTDIRDKSAHPENALTGHENEVISAQPETVIQNMDDLVTVVALVTLHTDALHATRD
                     EPLAFVPPGMAFRVFAGVAAGMTERGLARMQ"
     gene            447..818
                     /locus_tag="KJL99_004621"
     CDS             447..818
                     /locus_tag="KJL99_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBD4439042.1"
                     /translation="MADFDNLFDVALDLADKAIIRNMGIRAVITSGRLKGIMISGVFD
                     DPENISLVAGGVRIEDSLPSLFVKTADILRLCRNDSLMIGRESFCVDRITPDDGGCSY
                     IRLRREGLPGNVRAGRYYEGA"
     gene            805..>1275
                     /locus_tag="KJL99_004622"
     CDS             805..>1275
                     /locus_tag="KJL99_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBD4439043.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNLPAIKLNTTRRRAGEG
                     LRVGKYFFRGAFVQQLANGRWHVLRCLPEARFATGHDHQGRLRKNRLPVEVVKIP"
BASE COUNT          303 a          285 c          396 g          291 t
ORIGIN      
        1 gggctcccgt tttttattcg gacaggaaca gatatggcaa agaaagacga taacctgaaa
       61 cgtctgagag aactggcagc atcgctggga cgtgagccgg atatctcggg aagcgcagca
      121 gatattgcgc agcgtgtggc tgagctggag gaggaacttg ccaatatgga tgacactgac
      181 atccgggata agtctgccca cccggaaaat gcgctgaccg gacatgaaaa tgaggtgata
      241 tcagcgcagc cggagaccgt gattcagaat atggatgatc tggttacagt cgtggcactg
      301 gtgacgctgc ataccgatgc acttcatgcc acgcgggatg aacctctggc atttgtgccg
      361 ccgggaatgg cgttccgtgt ctttgccggt gtggcagccg gaatgacaga acgtggcctg
      421 gccagaatgc aataacggga ggcagcgtgg ctgatttcga taatctgttt gatgttgcac
      481 ttgatctcgc agacaaggcc attattcgca atatggggat tagagcggtc attacgtcag
      541 gccggctaaa agggatcatg atttccgggg tttttgatga tcctgaaaat atttctctgg
      601 tggccggcgg tgtgcgtatt gaagactctt taccatccct gtttgtgaaa acagcagata
      661 ttttacggct gtgtcgcaat gattcgctga tgattggtcg tgagtctttc tgtgtggatc
      721 gtatcacccc tgatgatggc ggatgtagtt atatccggtt gcggcgtgag gggctgccgg
      781 gaaacgtaag ggcaggacga tattatgaag gggcttgaga atgccatccg caatctgaac
      841 agccttgata cccgtatggt gccacaggcc agcgcatggg cgataaaccg tgtggcacag
      901 aaagcggtct cggttgccac ccggcaggtt gccgggaata ccgttgcggg cgataaccag
      961 gtgaaaggga tccccctgaa actggtacgt cagcgtgtcc gggtgtttaa agccagtccg
     1021 tcaggaaaaa tgacggccag gatccgcgtt aaccggggca atctgcccgc catcaaactg
     1081 aacacaacac ggcggcgtgc tggtgaagga ctgagagtgg gaaaatactt tttccggggg
     1141 gcatttgttc agcaactggc gaatggccgc tggcatgttc tgaggtgtct tcctgaagcg
     1201 cgttttgcaa cagggcatga ccatcagggc aggctaagaa aaaatcgtct tcctgtggag
     1261 gtagtgaaaa tcccg
//
LOCUS       DAEBXN010000340         1274 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.347, whole genome shotgun sequence.
ACCESSION   DAEBXN010000340 DAEBXN010000000
VERSION     DAEBXN010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.347"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..143
                     /locus_tag="KJL99_004623"
     CDS             <1..143
                     /locus_tag="KJL99_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBD4439044.1"
                     /translation="AEVELATLTWVDWYNNRRLPERLGHIPPAEAEKAYYASIGNDDL
                     SA"
     gene            127..486
                     /locus_tag="KJL99_004624"
     CDS             127..486
                     /locus_tag="KJL99_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4439045.1"
                     /translation="MICQPEFTDKTLSRKPGAVQKVNETDAEYLVRKFDELEAKCAAQ
                     ENKVIPVSTELPPANESVLLFDANGEGWLIGWRSLWYTWGQKETGEWQWTFQVGDLEN
                     VNITHWAVMPKAPEAGA"
     gene            486..791
                     /locus_tag="KJL99_004625"
     CDS             486..791
                     /locus_tag="KJL99_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4439046.1"
                     /translation="MTTFTDKELIKEIKERISSLDVRDDIERRAYEIALLSLEVEPDE
                     REAYELFMEKRFGDLVDRRRAKNGDNEYMAWDMTLGWIVWQQRAGIHFSTMSQQEVK"
     gene            791..1141
                     /locus_tag="KJL99_004626"
     CDS             791..1141
                     /locus_tag="KJL99_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBD4439047.1"
                     /translation="MEPYSLTLDEACHFLKISRPTAINWIRTGRLQATRKDPTKNKSP
                     YLTTRQACIAALQSPLHTVQVSAGDGITEERKCHSSAEVKYGTPVSHCRTVKDLNSLL
                     EQRTKGRRQNSMTS"
BASE COUNT          406 a          278 c          323 g          267 t
ORIGIN      
        1 gtgcagaagt ggaactggcc acactaacgt gggtggactg gtataacaat cgacgattgc
       61 cggaaaggct gggccacatc cctccggcag aagcagaaaa agcttattat gcttccatcg
      121 gaaacgatga tctgtcagcc tgagttcaca gataaaacac tctccaggaa acccggggcg
      181 gttcagaaag taaatgaaac agatgctgaa tacctggtgc gtaaattcga tgagcttgaa
      241 gctaaatgtg cagcacagga aaacaaagta ataccagtgt caactgaact gccaccagca
      301 aatgaaagtg ttttgttatt cgatgctaac ggagaaggct ggctaattgg ctggcgttct
      361 ctctggtaca cctggggaca aaaagaaacc ggagaatggc agtggacatt tcaggtcggg
      421 gaccttgaaa acgtcaatat cactcactgg gcagtaatgc caaaagcacc ggaggctgga
      481 gcataatgac cacttttacc gacaaagaac tgattaaaga aattaaagag cgtatcagca
      541 gccttgacgt gcgagacgat attgagcgcc gtgcttatga aatcgcactc ctatctctgg
      601 aagtagaacc agatgaacgc gaagcttatg aattattcat ggaaaagcgt tttggtgact
      661 tagtagatcg tcggagagca aaaaacggcg ataacgaata catggcatgg gatatgactc
      721 tcggttggat cgtctggcag caacgagctg gtatccattt ctcaacaatg tcacagcaag
      781 aggtgaaata atggagccat acagcctcac actcgatgag gcctgtcatt ttctcaagat
      841 atccagaccg actgccatta actggatacg cacagggcgt cttcaggcaa cacgcaaaga
      901 tcccactaag aataaatctc cttacctcac aacacgacaa gcctgcattg cggctcttca
      961 gtctccgctg catactgtcc aggtgagcgc gggtgatggc ataacagagg aaagaaaatg
     1021 tcactcttcc gcagaggtga aatatggtac gccagtttca cattgccgaa cggtaaaaga
     1081 tttaaacagt ctcttggaac aaaggacaaa aggcaggcga cagaactcca tgacaagcta
     1141 aaggctgaag catggcgggt cagcaaactt ggtgaaatac ctgatataac gttcgaggaa
     1201 gcgtgtgtca ggtggcttga agagaaagca cataaaaaat cactggacga tgacaaaagc
     1261 cggatcggat tctg
//
LOCUS       DAEBXN010000341         1258 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.348, whole genome shotgun sequence.
ACCESSION   DAEBXN010000341 DAEBXN010000000
VERSION     DAEBXN010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.348"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(<1..777)
                     /gene="ipaH3"
                     /locus_tag="KJL99_004627"
     CDS             complement(<1..777)
                     /gene="ipaH3"
                     /locus_tag="KJL99_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase IpaH3"
                     /protein_id="HBD4439048.1"
                     /translation="MSIMLPINNNFSLSQNSFYNTISGTYADYFSAWDKWEKQALPGE
                     NRNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNALISLPELPASL
                     EYLDACDNRLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINADNNQLTMLPELPT
                     SLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHSEETEIFFRCRENR
                     ITHIPENILSLDPTCTIILEDNPLSSRIRESLSQQTAQPDY"
BASE COUNT          366 a          210 c          305 g          377 t
ORIGIN      
        1 gtagtccggt tgggcggttt gttgcgacag agactccctg atccgtgagg acagaggatt
       61 gtcttcgagg atgatagtgc aggtcggatc aaggctaagt atattttccg gaatgtgtgt
      121 gatgcgattc tcgcggcacc ggaaaaatat ctcggtttcc tctgagtgat gatttcttac
      181 aggtacggct ggtaggcttt ccagaagatt agtacttaca tcgagcgctt ccagtgattc
      241 aggtaactca gggagaaatg tcagctggtt atttcttact gagagcactt ccagcgatgt
      301 aggtaattca ggaagcatgg ttagctgatt gttatctgca ttaatatatt ccagcaatgc
      361 aggcaattca ggaagcatgg ttagttggtt gttatctaca tcaagatgtt tcagagatgc
      421 gggtaattca ggaagtgttg acaggcgatt gtcacaggcg tcaaggtatt ccagcgatgc
      481 tggcaattct ggtaatgata ttagggcatt ctgagtaatt tccagaacag tgatttgagg
      541 tggtaagttg tcaggtagcg aggacagatt taaacgattc agttgaagct cactgaactg
      601 attgatgaga cattctttaa gtagggagac cgcttcattc cgattttcac cggggagcgc
      661 ttgtttttcc catttatccc atgctgaaaa gtaatcagca tatgtaccgg aaatagtgtt
      721 ataaaaagaa ttttgggaca atgaaaagtt attatttatc ggtaacatga tggacattca
      781 gttaaagccg atggacaaat ggctttgtta aaagaaataa gtaggagttt ttatgaagtt
      841 cattccatga aatgaacttc agcacttttt tattataaaa ggacaaacaa aattggctgt
      901 aaaaaaacga tactgtttca gttaactgtg aatagcttgc ttgcaccgta ttgttcttgt
      961 ttttcggtta gttaaacatc gtacttcata tttgaacgtt ctgccggaat gcattatcaa
     1021 tagaggtaaa gtcgcaaccc caaatcgtaa aggaaaccgt agcacgtcgt atgcaagaac
     1081 gtgccacggc tggctgatgg atgttcgata gcgcgagttt gaatgaaaat cagccggaga
     1141 tgattttaca taattgctac ggaattattc aatacaggaa ttgcttgcgt ctgcatggat
     1201 tgacctgaaa tattcccgaa aatttctcta aaaaactcga aaaaaatggt aactggtt
//
LOCUS       DAEBXN010000342         1257 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.349, whole genome shotgun sequence.
ACCESSION   DAEBXN010000342 DAEBXN010000000
VERSION     DAEBXN010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1257
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.349"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(<1..143)
                     /locus_tag="KJL99_004628"
     CDS             complement(<1..143)
                     /locus_tag="KJL99_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HBD4439049.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFT"
     gene            complement(545..>1257)
                     /gene="ydeO"
                     /locus_tag="KJL99_004629"
     CDS             complement(545..>1257)
                     /gene="ydeO"
                     /locus_tag="KJL99_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HBD4439050.1"
                     /translation="ILDKDYAFSDGEILMVDNAVRTHFEPYERHFKEIGFNENTIKKY
                     LQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERNHHNFSELLLFSCLSIFA
                     ACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCLYISESLLKKKLKQEQTT
                     FSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYAFRKHFGNSPKRVSKEYR
                     CQRHTGMNTGNTMSALAI"
BASE COUNT          391 a          236 c          238 g          392 t
ORIGIN      
        1 gtaaagtctg agaaagcaac gtttgttttg gttgctttca gctttacatc atctgccgca
       61 tgagcagcaa atgcagccat accagatgcc agtatcaaag atatcgaggt acttacgaca
      121 cttttcttta atgcagactt cataaatgtt cctcttctta ttgtacttaa ttccgaagta
      181 attttacgaa gagaaataag tggatgtaag tgaagttagt cacataaaga gatagcagat
      241 ttagctaaaa aaagggaaat aacagtccat aaagcgttga cattactttc tgttctatta
      301 agtaatttct cgccgataaa caactaattt attgatattt aataaattat tgcattttac
      361 tgacaaaatg ccgaattgag atcataaata atcatgcaac aggttatgca agtgcataaa
      421 tatgtgatgg atgtcactta tttatttcaa taaatatatc gcctaaaaac aacgcggggc
      481 agggaatggc tgccccatat aattcttacg tagcgtgcgt ggttgactac tcgttagcaa
      541 ataatcaaat agctaaagca ctcatcgtgt tgcccgtatt catacccgtg tgcctttgac
      601 aacggtactc cttagaaact ctcttcggac tgttgccgaa atgtttgcgg aacgcataaa
      661 taaaataaga tgtactggca taaccacatt gttcggcaat tttattgact gaaccttcta
      721 cgcgtatcaa attttttgcg tgctgcattc tggcatctaa tagaatctgt gagaatgtcg
      781 tttgctcttg ctttagtttt ttcttcaaca ggctttcact gatgtacagg cagtcacaaa
      841 tatctttcag cttccatggg tgcgccagct tcatgttgac aatatttctc actttcccgg
      901 aaacggatag cacaccgtta gttaatagtg taatgaaacc tttgcatgcg gcaaaaatag
      961 acaggcaaga aaaaagcaaa agttctgaaa aattatgatg attgcgttct tccgagttga
     1021 gataagcaat catttcatta agcaatccag tcggtacatt tgaagcacgt aaaaacttcg
     1081 caggaacagg caccgtcact gtctggatgt tagtgcattg tagatatttt ttaatggtat
     1141 tttcattaaa tccgatttct ttaaaatgcc gctcataagg ttcaaaatgt gtacgaacag
     1201 cgttatctac catcaggatc tcgccgtcgg agaaggcgta atctttatct aaaatgt
//
LOCUS       DAEBXN010000343         1219 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.350, whole genome shotgun sequence.
ACCESSION   DAEBXN010000343 DAEBXN010000000
VERSION     DAEBXN010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.350"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            296..>1219
                     /locus_tag="KJL99_004630"
     CDS             296..>1219
                     /locus_tag="KJL99_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HBD4439051.1"
                     /translation="MIKSTNIQVIGSGIMHQINNIHSLTLFSLPVSLSPSCNEYYLKV
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQGAELNLSELDLKTLPDLPPQITILEIRKNL
                     LTYLPDFPPMLKVIHAQFNQLESLPALPETLEELNVGDNKIKELPYLPETLTHLRIHN
                     NRLHILPLLPPELKLLIVSGNRLDSIPPFPDKLEGLALANNFIEQLPELPFSMNRAVL
                     MNNNLTTLPESVLRLAQNAFVNVAGNPLSGHTMRTLQQITTGPDYSGPRIFFSMGNSA
                     TISAPEHSLADAVTAWFPENKQSDVSQIWHAF"
BASE COUNT          389 a          269 c          235 g          326 t
ORIGIN      
        1 gagcaggtac cggcatccac gttacagacg gtcccggcaa aagtgcctga cctgacccct
       61 attagcggtt accagcgccc caccagaccc ggtgacatca ccccccggat catggacgac
      121 tgggggcagt aatcatgccg ggtctggctg gctaacaccc cattataagg ggtacgaata
      181 aagtaacggg ggaagcaata cctggagaaa gagtacaaag caaactgtat taaacttaat
      241 aagttatatt gtatttcact agagttctta tcgttgataa gaaattctgg ttataatgat
      301 taaatcaacg aatatacagg taatcggttc tggtattatg catcaaataa acaacataca
      361 ctcgttaact ctattttctt tgcctgtgag tttgagtcca tcttgtaatg aatattattt
      421 aaaagtctgg agtgaatggg aaaagaacgg taccccaggc gagcaacgca atatcgcctt
      481 caataggcta aaaatatgtt tgcaaaatca aggggcggaa ttaaatctat ctgagttaga
      541 tttaaaaaca ttacctgatt taccgcctca gataacaata ctggaaataa gaaaaaacct
      601 attaacatat ctccctgatt tcccaccaat gcttaaggta atacatgctc aatttaatca
      661 actggaaagc ttacctgcct tacccgagac gttagaagag cttaatgtgg gtgataacaa
      721 gataaaagaa ttaccatatc ttcctgaaac tctaactcat ttacggatcc ataataaccg
      781 attgcatatt ctgccactat tgccaccgga gctaaaatta ctgatagttt ctggaaacag
      841 attagacagc atccccccct ttccagataa gcttgaaggg ctggctctgg ctaataattt
      901 tatagaacaa ctaccggaat taccttttag tatgaacagg gctgtgctaa tgaataataa
      961 tctgacaaca cttccggaaa gtgtcctgag attagctcag aatgccttcg taaatgttgc
     1021 aggtaatcca ttgtctggcc ataccatgcg tacactacaa caaataacca ccggaccaga
     1081 ttattctggt cctcgaatat ttttctctat gggaaattct gccacaattt ccgctccaga
     1141 acactccctg gctgatgccg tgacagcatg gttcccggaa aacaaacaat ctgatgtatc
     1201 acagatatgg catgctttt
//
LOCUS       DAEBXN010000344         1212 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.351, whole genome shotgun sequence.
ACCESSION   DAEBXN010000344 DAEBXN010000000
VERSION     DAEBXN010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.351"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(130..273)
                     /locus_tag="KJL99_004631"
     CDS             complement(130..273)
                     /locus_tag="KJL99_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4439052.1"
                     /translation="MEHRSVIHDELAIDHDAILQCAEEVLHHCVIQTVSFVRHILCYS
                     MGL"
     gene            512..>1212
                     /locus_tag="KJL99_004632"
     CDS             512..>1212
                     /locus_tag="KJL99_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBD4439053.1"
                     /translation="MNKIYSLKYSAATGGLIAVSELAKRVSGKTNRKLVATMLSLAVA
                     GTVNAANIDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGA
                     AASGAATAIGGSYSVTVAHNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVV
                     ETVGATPAETNPTTYSDALERYGIVTSDGSKKIIGFRAGSGGTSFINGESKISTNSAY
                     SHDLLSASLFEVTQW"
BASE COUNT          398 a          242 c          243 g          329 t
ORIGIN      
        1 ataaagtccg gtaatggaga ttgttaatac gatggtgcac gatgcatcgt cagtgcccgg
       61 cccggaggat gccccgtagc tacatacgga tcagggttgg caatatcgaa tggcaggcta
      121 taggcaaagt taaagcccca tggagtagca caaaatatgc cgcacaaagg aaacggtctg
      181 aataacgcag tgatggagaa cttcttcggc acactgcaaa atcgcatcgt ggtcgatcgc
      241 taattcgtcg tgaatcactg aacgatgttc catgcgaaca gatattcgat catatcgaaa
      301 tattaaacat tacccctccc cacattgggc aaaaaaaaac aaaaaacgat caattagtat
      361 tgattcacat aatctctcat cattaatgag aacattgtca catatttaca atatagaact
      421 gttactttta gcaagctgtt agtaaccaac cgttactaat aattttaatt cttaatactt
      481 tgttcattca ataaatgagt tgagagaata tatgaataaa atatactccc ttaaatatag
      541 tgctgccact ggcggactca ttgctgtttc tgaattagcg aaaagagttt ctggtaaaac
      601 aaaccgaaaa cttgtagcaa caatgttgtc tctggctgtt gcaggtacag taaatgcagc
      661 aaatattgat atatcaaatg tatgggcgag agactatctt gatcttgcac aaaataaagg
      721 tattttccag cccggagcaa cagacgtaac aatcacttta aaaaacggag ataaattctc
      781 tttccataat ctctcaattc cggatttttc tggtgcagca gcgagtggcg cagctaccgc
      841 aataggaggt tcttatagtg ttactgttgc acataacaaa aagaaccctc aggccgcaga
      901 aactcaggtt tacgctcagt cttcttacaa ggttgttgac agaagaaatt ccaatgattt
      961 tgagattcag aggttaaata aatttgttgt ggaaacagta ggtgccaccc cggcagagac
     1021 caaccctaca acatattctg atgcattaga acgctacggt atagtcactt ctgacggttc
     1081 aaaaaaaatc ataggttttc gtgctggctc tggaggaaca tcatttatta atggtgaatc
     1141 caaaatctca acaaattcag catatagcca tgacctgtta agtgctagtc tatttgaggt
     1201 cacccaatgg ga
//
LOCUS       DAEBXN010000345         1196 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.352, whole genome shotgun sequence.
ACCESSION   DAEBXN010000345 DAEBXN010000000
VERSION     DAEBXN010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.352"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            151..>1196
                     /locus_tag="KJL99_004633"
     CDS             151..>1196
                     /locus_tag="KJL99_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HBD4439054.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRA"
BASE COUNT          384 a          277 c          190 g          345 t
ORIGIN      
        1 gaaccaaata tacgaaaaaa gcaaatatac ttaccagggt agaaagcatg atataacact
       61 cattccctta tggcaaatat tctgaaacac cctcctcatt aggagagata cattaaatac
      121 aatttattca caatctaatt ccatatccat atgcgagaaa ttaatatgct cagaaacatt
      181 tcatcctgtt tatttccaca tatcagcaca attacatccc ccaaccatta tttgtccgaa
      241 tgggatgatt gggagaaaca ggggttaccg gaagaacagc gtactgaggc ggtaagaaga
      301 cttcgtgcat gtcttacctc taaggggcat aaactggacc tgcgagcctt ggcgctttcc
      361 tcgctacctg tactccctgc ttgcattaaa aagcttgatg tgagctgtaa taaattaacc
      421 atccttactg atttacctga aaatattaaa gaacttattg caagagataa tttcttaaca
      481 catatatctg cattaccaca ttatctaata actttggatg tgtccgaaaa tcaattagag
      541 aatctgccgt tattaccaga caccatcaaa tcactaagcg cagagtataa taggttatcc
      601 acactgcctt cattaccctt gaatttaaaa aaacttgagg ttaggaacaa cgaactgcaa
      661 actcttccat ctctgccttc taatcttaag atacttaagg ttgcgcacaa ccatcttact
      721 gaactgcccc ctttacctag gagactgcaa cttctttttg catatagcaa tagattaagc
      781 aacttaccaa acatccaaga aaatattatc atgagaagat ttttttattt tgaaaacaac
      841 caaataacta caatcccgac aaatcttttt cgtttagatc ctcatataac tattgagatt
      901 gcaaataacc ccttatcaga tcaaactctg ctattcttaa tacagcaaac ttcggttcca
      961 aattttaacg ggcctcagtt tcgtatttcc ctgtcagacc aaaacagact gtttttacgc
     1021 cagatgttgc cgcaaaattt acattcgcgc catatcagag tcatcactga aggggggcag
     1081 aactttcaga tcccccctct tcccgaaact gtggcagcct ggtttcctga agcagatcgt
     1141 cgggaggttt ctacacaatg gacttctttt tccaccgagg agaattcccg ggcatt
//
LOCUS       DAEBXN010000346         1182 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.353, whole genome shotgun sequence.
ACCESSION   DAEBXN010000346 DAEBXN010000000
VERSION     DAEBXN010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1182
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.353"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            1..282
                     /locus_tag="KJL99_004634"
                     /pseudo
     CDS             1..282
                     /locus_tag="KJL99_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            299..817
                     /locus_tag="KJL99_004635"
                     /pseudo
     CDS             299..817
                     /locus_tag="KJL99_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          335 a          284 c          269 g          294 t
ORIGIN      
        1 gaacaaatgg tagtgtccgc tattgccagt acacctcacc tggtgtatat ccgcactcgc
       61 gaaacatatg gaacccggcg gctccagacg gagctggcag ataacggaat tatcgtcggt
      121 cgtgacagac tggcaggtct tcgtaaggag ctgaggctgc actgtaagca gaaacgcaag
      181 ttcagggcga ccacaaactc tgaccacaat ctgccggtta cgccgaatct gctgaatcag
      241 aactttacgc ccacagcacc taatcaggtc tgggtcgcag attcagttgt ccaggccttc
      301 cgaaatcaac cgactgaagg cgctggtcgc gaaactgcag cgtatgcagt tcggtaaaag
      361 ctcagaaaaa cttcgcgcaa aaaccgaacg gtggatacag gaagcacagg agcgaatcag
      421 cgcacttcag gaggaaatgg cggaaacact gggtgagcaa tatgacccgg tactgccatc
      481 ttccctgcgc cagtcttcag cccgtaaacc gttaccagcc tcacttcccc gtgccccccg
      541 ggtcatccgg ccggaagagg aatgctgtcc ggcctgtggt ggtgaactca gtccactggg
      601 atgtgatgtg tcagagcagc tggagcttat cagcagcgcc tttaaggtta tcgaaaaaca
      661 acgccctaaa ctggcctgtc gccggtgcga ccatatcgtg caggcaccgg tgccttcaaa
      721 acccattgca cgcagttatg ccggagcggg gcttctggcc catgttgtta ccggaaagta
      781 tgcagaccat ctgccattat accgccaaag tgatctacta ttccacacag ctatttagct
      841 ctctttttgt tgagctccca tgattctttc atttattgtt actaattatt ttatgttatc
      901 atgatgttag aacacgctct gctctgtgtc tctattttat ctatcatatt ctgaggatta
      961 actacataac aaggcgcaag atatatatac aaaaacaaat aataccgttg caataattcc
     1021 ttttagtaaa ggaggtgtga ttaatttcag gggatttcag agtaaaaatt gtacaacata
     1081 ttaaaatatg ttcatttcgt tataatacac acaaggaaat attaagttag ataatatatt
     1141 ctaaataacc atagataaaa acgcacataa tggggaatat tt
//
LOCUS       DAEBXN010000347         1160 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.354, whole genome shotgun sequence.
ACCESSION   DAEBXN010000347 DAEBXN010000000
VERSION     DAEBXN010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.354"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            116..>1160
                     /gene="cadB"
                     /locus_tag="KJL99_004636"
     CDS             116..>1160
                     /gene="cadB"
                     /locus_tag="KJL99_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HBD4439055.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKAS"
BASE COUNT          246 a          256 c          298 g          360 t
ORIGIN      
        1 ctttaggatt aatccttttt tcgtgagtaa tcttatcgcc agtttggtct ggtcaggaaa
       61 tagttataca tcatgacccg gactccaaat tcaaaaatga aattaggaga agagcatgag
      121 ttctgccaag aagatcgggc tatttgcctg taccggtgtt gttgccggta atatgatggg
      181 gagcggtatt gcattattac ctgcgaacct agcaagtatc ggtggtattg ctatctgggg
      241 ttggattatc tctattattg gtgcaatgtc gctggcgtat gtatatgccc gactggcaac
      301 aaaaaacccg caacaaggtg gcccaattgc ttatgccgga gaaatttccc ctgcatttgg
      361 ttttcagaca ggtgttcttt attaccatgc taactggatt ggtaacctgg cgattggtat
      421 taccgctgta tcttatcttt ccaccttctt cccagtatta aatgatcctg ttccggcggg
      481 tatcgcctgt attgctatcg tctgggtatt tacctttgta aatatgctcg gcggtacctg
      541 ggtaagccgt ttaaccacta ttggtctggt gctggttctt attcctgtgg tgatgactgc
      601 tattgttggc tggcattggt ttgatgcggc aacttatgca gctaactgga atactgcgga
      661 taccactgat ggtcatgcga tcattaaaag tattctgctc tgcctgtggg ccttcgtggg
      721 tgttgaatcc gcagcagtaa gtactggtat ggttaaaaac ccgaaacgta ccgttccgct
      781 ggcaaccatg ctgggtactg gtttagcagg tattgtttat atcgctgcga ctcaggtgct
      841 ttccggtatg tatccgtctt ctgtaatggc ggcttccggt gctccgtttg caatcagtgc
      901 ttcaactatc ctcggtaact gggctgcgcc gctggtttct gcattcaccg cctttgcgtg
      961 cctgacttct ctgggctcct ggatgatgtt ggtaggccag gcaggtgtac gtgccgctaa
     1021 cgacggtaac ttcccgaaag tttatggtga agtcgacagc aacggtattc cgaaaaaagg
     1081 tctgctgctg gctgcagtga aaatgactgc cctgatgatc ctcatcactc tgatgaactc
     1141 tgccggtggt aaagcatctg
//
LOCUS       DAEBXN010000348         1144 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.355, whole genome shotgun sequence.
ACCESSION   DAEBXN010000348 DAEBXN010000000
VERSION     DAEBXN010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.355"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..734
                     /locus_tag="KJL99_004637"
     CDS             <1..734
                     /locus_tag="KJL99_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HBD4439056.1"
                     /translation="KTDRIHLKDAARMLEVSVMTIRRDLHQEDEPLPLTLLGGYIVMV
                     NKPAPSMPVIHDVPKNHRDDLPIAILAAGMVNENDLIFFDNGQEIPLVISMIPDAITF
                     TGICYSHRVFVALNEKPNVTAILCGGTYRARSDAFYDASNSSPLDSLNPRKIFISASG
                     VHNHFGVSWFNPEDLATKRKVMNRGLRKILLARHALFDEVASASLAPISAFDVLISDR
                     PLPADYVTHCQNGSVKIITPDSEDE"
     gene            complement(736..962)
                     /locus_tag="KJL99_004638"
                     /pseudo
     CDS             complement(736..962)
                     /locus_tag="KJL99_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229544.1"
                     /note="with IlvB catalyzes the formation of 2-acetolactate
                     from pyruvate, the small subunit is required for full
                     activity and valine sensitivity; E.coli produces 3
                     isoenzymes of acetolactate synthase which differ in
                     specificity to substrates, valine sensitivity and affinity
                     for cofactors; also known as acetolactate synthase small
                     subunit; frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
BASE COUNT          301 a          283 c          248 g          312 t
ORIGIN      
        1 agaaaaccga cagaatccat ttgaaagacg cagcgcgaat gctggaagtt tctgtaatga
       61 ctattcgtcg cgatctccat caggaagatg aacctctgcc actgacccta ctgggtggct
      121 atattgtaat ggtgaataaa cccgcgccat ccatgccagt aatccatgac gttccaaaaa
      181 atcatcgtga tgacttacct attgcaattc tggctgccgg aatggttaat gaaaatgatc
      241 tgatcttctt tgataatggc caggagatac cactcgttat aagcatgatc ccggatgcaa
      301 tcaccttcac cggcatctgt tactcacatc gcgtctttgt tgcgttgaat gaaaagccta
      361 atgtaacagc aatactttgt ggtggtacgt atcgtgccag aagtgatgct ttttacgatg
      421 ccagtaactc ttcgccatta gactctctca atccgcgaaa aatatttatt tccgccagcg
      481 gtgtgcataa tcactttggc gtcagctggt ttaaccctga agatcttgcc actaagcgta
      541 aagtgatgaa ccgtggacta cggaaaattt tgctcgcccg ccacgcgttg ttcgatgaag
      601 tggcctctgc cagcctcgca ccgatctctg catttgacgt tctgattagc gatcgtccgt
      661 taccggcaga ttatgttacg cactgccaga atggttctgt aaagatcatt acacctgatt
      721 cagaagacga atgacttact gaaaaaacac cacaatcttg ttaaacatcg tcgggttgga
      781 ctgattacgt tgcactttca ccacatattc cagcttatct atttggctta tcacctactc
      841 cagacgctgg tcatccttga ccagtagcca gatatggctt ttgtcgcagt cctgaatcgg
      901 caggcagaga atgtcttcaa cgttaagagc gcgacgggca aaagcccaca aaggtgagtc
      961 attacacccg gatggtaaac aggcacttcc ttgttgtaga agccgttata ttgtccataa
     1021 tgtctgggtt tttaatgcat actgggtaac aaccggacga atcttcgaat atcactggcg
     1081 ctgccagccg gtttgaattc atctcacaac cctgcataga gcgaatctcc tgcctgcacg
     1141 tcac
//
LOCUS       DAEBXN010000349         1109 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.356, whole genome shotgun sequence.
ACCESSION   DAEBXN010000349 DAEBXN010000000
VERSION     DAEBXN010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.356"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            1..1019
                     /locus_tag="KJL99_004639"
                     /pseudo
     CDS             1..1019
                     /locus_tag="KJL99_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
BASE COUNT          312 a          271 c          258 g          268 t
ORIGIN      
        1 aattctcttg caccattaac tatgttcagt ccctgcgcga gttgtacacc gatgtcatcg
       61 aagagtttta ctgggttgcg ctcccgctta cgacgcaaaa ttccctttca caataccaac
      121 ccgaatggca gtgctgggaa cctgatgtcg aatgggtgcg tcagcccccg caagatgcga
      181 taaccgctcc tgactttttc tgcttttacc agccaggcat gaccttcgaa caatttgtac
      241 gtgagtttgc cgaatggttt tcacaaaaac gtccggcggc gatgatgatc ggcatccgtg
      301 cggatgagtc ctacaaccgt tttgtcgcca tcgccagttt aaataaacaa cgttttgccg
      361 acgataaacc atggactacg gccgcaccag gcggtcatag ctggtacatt taccccattt
      421 acgactggaa agtggctgat atctggacct gatatgcaaa tcatcaaaat ctctgcaacc
      481 cactgtataa cctaatgtat caggcaggcg ttcctctgcg tcatatgcga atttgcgaac
      541 cttttggccc ggagcaacga caaggattat ggctctgtca cgttatcgaa ccggatcgct
      601 gggctgctat gtgcgcacga gtcagcgggg taaaaagtgg cggcatttac gccggacatg
      661 acaatcattt ctatgggcat cggaaaatcc tcaaaccaga acatttagac tggcaagaat
      721 atgcattatt gctgctcaat agcatgccgg aaaaaacagc tgagcattac cgcaataaaa
      781 tcgccattta tttgcactgg tatcagaaaa aaggcatcga ggttccacaa acccagcaag
      841 gggacattgg cgcgaaagat atcccctcct ggcggcggat atgcaaagtt ttactcaata
      901 acgattactg gtgtcgggca ttatcattta gtcctacgaa agcgaagaac tatcagcgtt
      961 ataacgaacg gataaaagga aaacgtcagg aatgggggat actatgcaac aacgattaac
     1021 gcaggattta acgcaatttc tcgccagctt gccggaagat gatcgcatca aagcgattaa
     1081 tgaaatccgc atggcgatcc atcaggtta
//
LOCUS       DAEBXN010000350         1103 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.357, whole genome shotgun sequence.
ACCESSION   DAEBXN010000350 DAEBXN010000000
VERSION     DAEBXN010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.357"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            1..>1103
                     /locus_tag="KJL99_004640"
     CDS             1..>1103
                     /locus_tag="KJL99_004640"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HBD4439057.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKQGPYEFICT"
BASE COUNT          260 a          294 c          295 g          254 t
ORIGIN      
        1 atggaccaga agctgttaac ggatttccgc tcagaactac tcgattcacg ttttggcgca
       61 aaggccattt ctactatcgc ggagtcaaaa cgatttccgc tgcacgaaat gcgcgatgat
      121 gtcgcattcc agattatcaa tgatgaatta tatcttgatg gcaacgctcg tcagaaccta
      181 gccactttct gccagacctg ggacgacgaa aacgtccata aattgatgga tttgtcgatc
      241 aataaaaact ggatcgacaa agaagaatat ccgcaatccg cagccatcga cctgcgttgc
      301 gtaaatatgg ttgccgatct gtggcatgcg cctgcgccga aaaatggtca ggccgttggc
      361 accaacacca ttggttcttc cgaggcctgt atgctcggcg ggatggcgat gaaatggcgt
      421 tggcgcaagc gtatggaagc tgcaggcaaa ccaacggata aaccaaacct agtgtgcggt
      481 ccggtacaaa tctgctggca taaattcgcc cgctactggg atgtggagct gcgtgagatc
      541 cctatgcgcc ccggtcagtt gtttatggac ccgaaacgca tgattgaagc ctgtgacgaa
      601 aacaccatcg gcgtggtgcc gactttcggc gtgacctaca ccggtaacta tgagttccca
      661 caaccgctgc acgatgcgct ggataaattc caggccgaca ccggtatcga catcgacatg
      721 cacatcgacg ctgccagcgg tggcttcctg gcaccgttcg tcgccccgga tatcgtctgg
      781 gacttccgcc tgccgcgtgt gaaatcgatc agtgcttcag gccataaatt cggtctggct
      841 ccgctgggct gcggctgggt tatctggcgt gacgaagaag cgctgccgca ggaactggtg
      901 ttcaacgttg actacctggg tggtcaaatt ggtacttttg ccatcaactt ctcccgcccg
      961 gcgggtcagg taattgcaca gtactatgaa ttcctgcgcc tcggtcgtga aggctatacc
     1021 aaagtacaga acgcctctta ccaggttgcc gcttatctgg cggatgaaat cgccaaacag
     1081 gggccgtatg agttcatctg tac
//
LOCUS       DAEBXN010000351         1103 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.358, whole genome shotgun sequence.
ACCESSION   DAEBXN010000351 DAEBXN010000000
VERSION     DAEBXN010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.358"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(22..825)
                     /gene="dkgB"
                     /locus_tag="KJL99_004641"
     CDS             complement(22..825)
                     /gene="dkgB"
                     /locus_tag="KJL99_004641"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HBD4439058.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKVIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNAIPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(988..1064)
                     /locus_tag="KJL99_004642"
     tRNA            complement(988..1064)
                     /locus_tag="KJL99_004642"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1028..1030),aa:Asp,seq:gtc)
BASE COUNT          286 a          259 c          252 g          306 t
ORIGIN      
        1 cggaggagct gtcagagagg cttaatccca ttcaggagcc agaccttccg ggctaaccag
       61 gcggtcgttg caatccagtg cggcgatcgc ttttttatct tcggcatcaa gctgtaaatt
      121 ttgtgcctta agattacttt ccaggttttt acgtttagta gaagaaggaa ttactgagta
      181 accttccccc atagcccacg ccagaatcac ttgtgccgga atcgcattgt gtttagctgc
      241 aatacgagca ataacctcat ctttcagtgc cttaccataa gccagcgtca tataggaagt
      301 gatatgaata ccgtgctgtt tagcccaggc aaccacttta cggttttgca gataaggaga
      361 gagttcaatc tggttggtag cgatgttttc agcgccaaca gcagcaatca ctttttccat
      421 caatgggatc gtgaagttgg aaataccgat ctcacgcgtc agcccttgtt ttttggcttc
      481 cagcagcgcc tgcataaact cttcaacaga gacttcatcg tttggtgacg gccagtggat
      541 tagcgtcaga tcaacataat cggtacgcaa tttttgcagg ctctctttca gactcgggat
      601 caatttgtct ttgctgagat tttcaatcca gattttagtg gtgatgtaga gttcatgacg
      661 tggcacgcca ctttctgcaa tcgcctgacc tactgcggct tcgttatcat agatttgtgc
      721 ggtatcaatt gcgcgataac caagttcaag cgccgttttc acagatgaaa taacaacgtc
      781 gtctttcaga cggaaagtac ctaaaccaaa tgcagggata gccataatat gcctctttta
      841 atgctatttt cgttaactga ggtgattatg ggcttcagac tgttgcggga aaagagcgta
      901 aaacgcagag gatttttgcg attctagcaa taatagatat ccagacgcaa aaaaacatcg
      961 agcgttaact cgaggcttct taataagtgg cggaacggac gggactcgaa cccgcgaccc
     1021 cctgcgtgac aggcaggtat tctaaccgac tgaactaccg ctccaccgaa ttcttttaca
     1081 accaccggtt ttatgaccgg ctt
//
LOCUS       DAEBXN010000352         1078 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.360, whole genome shotgun sequence.
ACCESSION   DAEBXN010000352 DAEBXN010000000
VERSION     DAEBXN010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.360"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            323..919
                     /gene="fimE"
                     /locus_tag="KJL99_004643"
     CDS             323..919
                     /gene="fimE"
                     /locus_tag="KJL99_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HBD4439059.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
BASE COUNT          321 a          193 c          216 g          348 t
ORIGIN      
        1 tttatgttct tcaaaataaa taaacaaaat cattcataag tttacacatc acttaaattc
       61 tcctgtttcc acactttttt ctttattttt taagtaactg gaagttaatc ctctgtaatc
      121 tattgttata ttgaatcaaa tcaatgaaaa tagatgttgt cacatcagtg atattttatt
      181 tttgtatgat gtttaatgta attgactgat agccacataa cttcgtgtgt ggttatcttt
      241 ttatctattg ggctaatttt gaccgattga ggtttcctat aggtattcat tcaaatatat
      301 ctcagttagg agtactacta ttgtgagtaa acgtcgttat cttaccggta aagaagttca
      361 ggccatgatg caggcggttt gttacggggc aacgggagcc agagattatt gtcttattct
      421 gttggcatat cggcatggga tgcgtattag tgaactgctt gatctgcatt atcaggacct
      481 tgaccttaat gaaggtcgaa taaatattcg ccgactgaag aacggatttt ctaccgttca
      541 cccgttacgt tttgatgagc gtgaagccgt ggaacgctgg acccaggaac gtgctaactg
      601 gaaaggcgct gaccggaccg acgctatatt tatttctcgc cgcgggagtc ggctttctcg
      661 ccagcaggcc tatcgcatta ttcgcgatgc cggtattgaa gctggaaccg taacgcagac
      721 tcatcctcat atgttaaggc atgcttgcgg ttatgaactg gcggagcgtg gtgcagatac
      781 ccgtttaatt caggattatc tcgggcatcg aaatattcgc catactgtgc gttataccgc
      841 cagtaatgct gctcgttttg ccggattatg ggaaagaaat aatctcataa acgaaaaatt
      901 aaaaagagaa gaggtttgat ttaacttatt gataataaag ttaaaaaaca aataaacaca
      961 agacaattgg ggccaaactg tccatatcat aaataagtta cgtatttttt ctcaagcata
     1021 aaaatattaa aaaacgacaa aaagcatcta actatttgat atgtaaatta tttctctt
//
LOCUS       DAEBXN010000353         1072 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.361, whole genome shotgun sequence.
ACCESSION   DAEBXN010000353 DAEBXN010000000
VERSION     DAEBXN010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1072
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.361"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(344..1039)
                     /gene="aqpZ"
                     /locus_tag="KJL99_004644"
     CDS             complement(344..1039)
                     /gene="aqpZ"
                     /locus_tag="KJL99_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HBD4439060.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
BASE COUNT          303 a          322 c          251 g          196 t
ORIGIN      
        1 tttaatccta tctgagtgtt tagcattgtt gcgctccttt catgtgcaat gccatataaa
       61 gccactggcg gagggagcgc gcagggggca gccagtcgcc gccgccccct gcacccccgg
      121 gctctggcga acaaaatcgc cgctgcgcgg taccttcagc ttatcccctt cggcaaccgg
      181 gtcgggcgcg aggtaacgtc ctgtaagacg cgccctcagc ccacatccat gtgggctgcc
      241 ccggccttcg gggaacgctt cagcgatttt gacgccacca atacccgtgc ggtttgtaat
      301 gtcgaagata aaacaaccag ggcagataaa atgccaatgc taattaatca cgcttttcca
      361 gcagagtccg gtaaatcaga ccaccgataa tgccgccgac aattggcacc acccagaaga
      421 accacagttg ttctaatgcc cagccgccct ggaagatagc aaccgcggtg ctgcgcgccg
      481 ggttaacaga agtgttagtc accggaatac tgattaagtg aatcagggtt aaggccagac
      541 caatagcgat cggcgcaaaa cctgccggag cgaatttgtc ggttgcaccg tggatcacta
      601 acaggaagcc cgcactcaat accagttcaa ctaccagcgc ggaaagcatg gaataaccgc
      661 ctggtgaatg ctcgccataa ccgttagaag caaaaccgct ggctgccgcg tcaaaacccg
      721 ttttaccact ggcaattaaa tacagcagcg ccgctgcaac aataccgccg acaacctggg
      781 caattacgta gccaacgact tcttttgccg gaaaacgtcc gccagcccat aaaccaatag
      841 tgaccgccgg gttaaaatga ccaccagaaa tatgaccaac agcgaaggcc atcgtcagaa
      901 cggtcaaacc aaatgccagc gccacgccgg caaaaccaat gcctaattcc gggaagcctg
      961 cggccagtac agcactacca cagccaccaa aaacaagcca gaaagtacca aaacattcag
     1021 ctgctaattt tctgaacata tccacctcaa ttaaaaattg accctgtgaa aa
//
LOCUS       DAEBXN010000354         1068 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.362, whole genome shotgun sequence.
ACCESSION   DAEBXN010000354 DAEBXN010000000
VERSION     DAEBXN010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.362"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            18..560
                     /locus_tag="KJL99_004645"
     CDS             18..560
                     /locus_tag="KJL99_004645"
                     /inference="COORDINATES: protein motif:HMM:NF024120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4439061.1"
                     /translation="MIGVKQPLPKAVLRTQHDKNKEAISILDFGVIDDGVTDNYQAIQ
                     NAIDAVASLPSGGELFIPASNQAVGYIVGSTLLIPGGVNIRGVGKASQLRAKSGLTGS
                     VLRLSYDSDTIGRYLRNIRVTGNNTCNGIDTNITAEDSVIRQVYGWVFDNVMVNEVET
                     ALFNARALALKIYSMSGWNL"
     gene            787..>1068
                     /locus_tag="KJL99_004646"
     CDS             787..>1068
                     /locus_tag="KJL99_004646"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endorhamnosidase"
                     /protein_id="HBD4439062.1"
                     /translation="MEQLDLDYCGSTGVVIENVNGGFSFSNSWIAADADGTEQFTGIY
                     FRTPASTQSHKIVSGVHINTANKNTAANNQSIAIEQSAIFVFVSGCTLTG"
BASE COUNT          314 a          202 c          257 g          295 t
ORIGIN      
        1 acaccaaagg cgatgccatg attggcgtca agcagccttt accaaaagca gttttaagaa
       61 ctcagcatga caaaaataaa gaagcaataa gtatcctgga ttttggtgtt attgatgatg
      121 gtgtgacaga taattaccag gcaatacaaa atgctataga tgccgttgct tcactaccct
      181 ccggcgggga gctgtttatc cctgcgagca accaagcggt ggggtatatt gttggatcca
      241 ctttgcttat tcctggcggt gttaacatca gaggagttgg taaggcatcg caactccgag
      301 caaaaagcgg acttacagga tctgtgttaa ggctgtctta tgattcagac actatcggcc
      361 gttatctgag aaatatacga gtaactggta ataacacctg caatggtatt gacacaaaca
      421 ttacagcaga agactctgtc atcagacagg tttatggctg ggtatttgat aatgtaatgg
      481 tgaatgaagt tgaaaccgca ttatttaatg caagggctct ggcactcaaa atttatagca
      541 tgtcaggctg gaacctgtag agtcggtctt cactttttag gccagtgcgt aagtgttagt
      601 gtcagctcct gccatttcag cagaggaaat tattctgctg atgaaagctt tggcatcagg
      661 attcagcctc aaacgtatgc gtggtcgtca gaggcagtta ggtcagaagc aataatttta
      721 gacagtgaga ccatgtgcat tggttttaaa aatgccgtct atgttcatga ttgccttgat
      781 ttgcatatgg aacaactgga tttagattat tgcggctcaa caggcgtggt tatagagaat
      841 gtaaacggag gattttcttt ctcaaactca tggatagcag cagatgccga tggcactgaa
      901 caatttacgg ggatatattt tagaacgccg gcctcaacgc agtcacataa aattgtcagt
      961 ggtgttcata tcaacactgc aaacaaaaac acggctgcaa acaatcaaag tatagcgata
     1021 gaacagtcgg cgatcttcgt ctttgtaagt ggttgtacgt taactggt
//
LOCUS       DAEBXN010000355         1066 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.363, whole genome shotgun sequence.
ACCESSION   DAEBXN010000355 DAEBXN010000000
VERSION     DAEBXN010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.363"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(570..749)
                     /locus_tag="KJL99_004647"
                     /pseudo
     CDS             complement(570..749)
                     /locus_tag="KJL99_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            843..>1066
                     /locus_tag="KJL99_004648"
     CDS             843..>1066
                     /locus_tag="KJL99_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBD4439063.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     QLFLWRKQYQEGSLTAVAAGEQVVPASELAA"
BASE COUNT          285 a          193 c          277 g          311 t
ORIGIN      
        1 ttagggtagt cactatattt gcgactgagc tggtcgctat ttttaaccag gctgaaaaat
       61 tgagtcaata tatcgaaagc ggtagttggc gggcataagt atgtaatgat gatgctgcac
      121 tggtgttgaa tattattcac gccaaacttt cctcattgag caatgtcgtc gcaagtggcc
      181 tcaatgattt tagaccatta tcagaggtcc ggagcggcgg gaagagattt attgctgtgg
      241 aataattgtg gtactgatga ttatttatat cccggattgg tagggaaaag cttttttcat
      301 gcactatttt tgcaaatatt tctatatggg aagtggtttt gttacagatt aaatttataa
      361 agaaatgcat ttggggtgtt gggttgaaga gtagctggta gatgttattg tgccagcagg
      421 ggcaggggaa tgtgccgtat cgatatgctt tggcaattgc gtgtgccgca gatggagatg
      481 aaatgccaat aaaaaagcct gagttcgatg agggacgaac tcaggctggt gttatgtttt
      541 acagtaacac gcctcggcgc gttggctgat tagaactggt agacgatacc aactgccagt
      601 tggtcgtcgg tgttcagtcc atgagcgaga acataatcac tcttatcaag caggttgatc
      661 tgataagcgg tatatatgtt catattctta ttgaagtaat accaggtacc gatttcaata
      721 tagttcagac gatcagaatc tttgtaacct gtagactggc cccctgaatc tccagacaac
      781 caatatcact tatttaagtg atagtcttaa tactagtttt tagactagtc attggagagc
      841 agatgattga tgtcttagga ccggagaaac gcagacggcg tactacacag gaaaagatcg
      901 ctattgttca gcagagtttt gaaccgggaa tgacggtctc ccttgttgcc cggcaacacg
      961 gtgtggcagc cagccagtta tttctctggc gcaagcaata ccaggaggga agtcttactg
     1021 ctgtggccgc aggagaacag gtcgttcctg cctctgaact tgctgc
//
LOCUS       DAEBXN010000356         1045 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.365, whole genome shotgun sequence.
ACCESSION   DAEBXN010000356 DAEBXN010000000
VERSION     DAEBXN010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.365"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..98
                     /gene="ospE1"
                     /locus_tag="KJL99_004649"
     CDS             <1..98
                     /gene="ospE1"
                     /locus_tag="KJL99_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 3 secretion system effector OspE1"
                     /protein_id="HBD4439064.1"
                     /translation="TLKFTATEMQWLTKINEAGIDEKQSQRYSDF"
     gene            489..>1045
                     /locus_tag="KJL99_004650"
     CDS             489..>1045
                     /locus_tag="KJL99_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HBD4439065.1"
                     /translation="MIKSTNIQAIGSGIMHQINNVYSLTPLSLPMELTPSCNEFYLKT
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTHLPDLPPMLKVIHAQFNQLESLPALPETLEELNAGDNKIKELPFLPENLTHLRVHN
                     NRLHILPLLPPELKLLVVSGNRLDS"
BASE COUNT          380 a          197 c          172 g          296 t
ORIGIN      
        1 aaacgttgaa attcactgct acagaaatgc aatggttgac aaagataaat gaagccggaa
       61 ttgatgaaaa acaaagccaa cggtattctg atttctgaag ttaatttcag actcgtttaa
      121 gctccatttt caaaaaaaat atatcagggt ttgttactaa taacttctga aaacagaaaa
      181 aaaatagagc agtaattcta tttcagagag gagtaactcc atctatcctc gatacgtact
      241 gaacgttatc gtgatatatt catcatgctt tcttcaccat tattcgagta tagggagaga
      301 cgcaatgacc ttaaatacct ttttatggct gaattaccat tctcaggaat aacagcagac
      361 tccttcccat agacggctta gggggaaagc aatacccgga gaaagggtac aaagcaaact
      421 gttttaaaat taataagcca tattctattt aactagaatt cttatcgttg ataagaattc
      481 tagttataat gattaaatca accaatatac aggcaatcgg ttctggcatt atgcatcaaa
      541 taaacaatgt atactcgtta actccattat ctttacctat ggaactgact ccatcttgta
      601 atgaatttta tttaaaaacc tggagcgaat gggaaaagaa cggtacccca ggcgagcaac
      661 gcaatatcgc cttcaatagg ctgaaaatat gtttacaaaa tcaagaggca gaattaaatt
      721 tatctgagtt agatttaaaa acattaccag atttaccgcc tcagataaca acactggaaa
      781 taagaaaaaa cctattaaca catctccctg atttaccacc aatgcttaag gtaatacatg
      841 ctcaatttaa tcaactggaa agcttacctg ccttacccga gacgttagaa gagcttaatg
      901 cgggtgataa caagataaaa gaattaccat ttcttcctga aaatctaact catttacggg
      961 ttcataataa ccgattgcat attctgccac tattgccacc ggaactaaaa ttactggtag
     1021 tttctggaaa cagattagac agcat
//
LOCUS       DAEBXN010000357         1044 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.366, whole genome shotgun sequence.
ACCESSION   DAEBXN010000357 DAEBXN010000000
VERSION     DAEBXN010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.366"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            76..>1044
                     /locus_tag="KJL99_004651"
     rRNA            76..>1044
                     /locus_tag="KJL99_004651"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          294 a          224 c          315 g          211 t
ORIGIN      
        1 aacgaaagtt gttcgtgagt ctctcaaatt ttcgcaacac gatgatgagt cgaaagaaac
       61 atcttcgggt tgtgaggtta agcgactaag cgtacacggt ggatgccctg gcagtcagag
      121 gcgatgaagg acgtgctaat ctgcgataag cgtcggtaag gtgatatgaa ccgttataac
      181 cggcgatttc cgaatgggga aacccagtgt gattcgtcac actatcatta actgaatcca
      241 taggttaatg aggcgaaccg ggggaactga aacatctaag taccccgagg aaaagaaatc
      301 aaccgagatt cccccagtag cggcgagcga acggggagca gcccagagcc tgaatcagtg
      361 tgtgtgttag tggaagcgtc tggaaaggcg tgcgatacag ggtgacagcc ccgtacacaa
      421 aaatgcacat actgtgagct cgatgagtag ggcgggacac gtggtatcct gtctgaatat
      481 ggggggacca tcctccaagg ctaaatactc ctgactgacc gatagtgaac cagtaccgtg
      541 agggaaaggc gaaaagaacc ccggcgaggg gagtgaaaaa gaacctgaaa ccgtgtacgt
      601 acaagcagtg ggagcacgct taggcgtgtg actgcgtacc ttttgtataa tgggtcagcg
      661 acttatattc tgtagcaagg ttaaccgaat aggggagccg aagggaaacc gagtcttaac
      721 tgggcgttaa gttgcagggt atagacccga aacccggtga tctagccatg ggcaggttga
      781 aggttgggta acactaactg gaggaccgaa ccgactaatg ttgaaaaatt agcggatgac
      841 ttgtggctgg gggtgaaagg ccaatcaaac cgggagatag ctggttctcc ccgaaagcta
      901 tttaggtagc gcctcgtgaa ttcatctccg ggggtagagc actgtttcgg caagggggtc
      961 atcccgactt accaacccga tgcaaactgc gaataccgga gaatgttatc acgggagaca
     1021 cacggcgggt gctaacgtcc gtcg
//
LOCUS       DAEBXN010000358          990 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.367, whole genome shotgun sequence.
ACCESSION   DAEBXN010000358 DAEBXN010000000
VERSION     DAEBXN010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..990
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.367"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            82..>990
                     /locus_tag="KJL99_004652"
     CDS             82..>990
                     /locus_tag="KJL99_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl1 family transposase"
                     /protein_id="HBD4439066.1"
                     /translation="MPVHKVCQNFFRDALAPFHQYRQNALMDATMALINGASLTQTSI
                     GRFLPGNAQVKNKIKRIDRLMGNEALHRDIPMIFRNITSMLTRQLSLCVIAVDWSGYP
                     SQEHHVLRASLLCDGRSIPLLSKVVPSEKQNNPLIQHDFLDSLAQSLPPDARVIIVTD
                     AGFQSAWFHHITSLGWDFIGRIRNNVQYCLDNAPERWLKVSDSPECKTPEYMGAGRLV
                     KERKKSIRGHFYTYKKSAKGRKKKRSKGQSGLNKTDKEQSKSAKEAWLIFSSTNDFRA
                     REIIKLYSRRMQIEQNFRDEKNGRFGF"
BASE COUNT          301 a          206 c          228 g          255 t
ORIGIN      
        1 tgaggaatcc ccagaaaacg agacaaacat agcattatgt gatctactga tttgtgccaa
       61 caattcaatg agatcactgc tatgcctgtt cataaagtat gtcagaattt cttccgggat
      121 gccttagcac catttcatca gtaccgacaa aatgccctga tggatgccac aatggcgtta
      181 atcaacggag catctctgac gcagaccagt atcggacgtt ttttgccggg taatgcacaa
      241 gttaagaata agatcaaacg tattgatcgc ctcatgggta atgaggcgct ccatcgcgat
      301 attccaatga ttttcaggaa tatcacttca atgctgacac ggcagttatc tttatgtgta
      361 atagccgtag actggagtgg ctatccctcc caggagcatc atgttcttcg tgccagcctt
      421 ctgtgtgacg ggcgttccat tccgttattg agtaaggttg ttccttctga aaaacaaaat
      481 aatccattga tacaacatga tttccttgat tcccttgctc aatccctgcc ccccgatgcc
      541 agggttatta ttgtcacgga tgctggattt cagagcgcat ggtttcacca tattacttct
      601 ctgggctggg attttattgg tcggatcaga aataacgttc agtattgcct cgataacgcg
      661 cctgaaagat ggttaaaagt gtcggatagc cctgaatgta aaacaccgga atatatgggg
      721 gctggaaggc tggttaaaga aaggaaaaaa agcatccggg ggcattttta cacctataag
      781 aaatcggcaa aagggcgaaa aaagaaacgt tcgaaaggtc agagtggttt aaacaaaaca
      841 gacaaagaac agagcaaatc agcaaaagaa gcctggttga tcttcagcag tacaaatgac
      901 ttcagagcgc gagaaatcat caagttatac agtcggcgaa tgcagataga gcaaaacttt
      961 cgtgatgaaa agaacgggcg cttcggattt
//
LOCUS       DAEBXN010000359          947 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.369, whole genome shotgun sequence.
ACCESSION   DAEBXN010000359 DAEBXN010000000
VERSION     DAEBXN010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..947
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.369"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(30..530)
                     /locus_tag="KJL99_004653"
     CDS             complement(30..530)
                     /locus_tag="KJL99_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4439067.1"
                     /translation="MLAIMLAGTAFASQAGTLVSQGTEASANLTLTKPIVVNNTIQPV
                     KGVYSGTLTAWTPLATGIVGASDGQSHDYAVTFPDDIYAESSTSADAVISGDNNPDHK
                     LKVSLTTLEQDPPSAASEEIGGKRYMMLKNTGTGGAYRVVSHMKEQVVEPDSYTIRTQ
                     AYIYAE"
     gene            complement(681..>947)
                     /locus_tag="KJL99_004654"
     CDS             complement(681..>947)
                     /locus_tag="KJL99_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBD4439068.1"
                     /translation="SGDKGLVTLKQKEGKPIPFGAVISYSKDTENMAGIVGEDGIAYV
                     SGLSAEGEFNVKWGYSKDQSCIAKYQLPAKKSASGLYQIAATCL"
BASE COUNT          258 a          230 c          175 g          284 t
ORIGIN      
        1 cccgccatga aattaactaa tcatctgcat tattctgcgt agatataagc ctgggtacgg
       61 atggtgtaag agtctggctc aacaacctgt tctttcatat gggaaacgac gcgataggca
      121 ccacctgttc cagtattttt cagcatcata taacgcttac cgccaatctc ttcggaggca
      181 gctgaaggag gatcctgctc aagcgttgtc agggaaactt tcagtttatg gtccgggtta
      241 ttgtcaccag aaattactgc atccgctgaa gtactgctct ccgcataaat atcatccggg
      301 aaagttacag catagtcgtg gctctgccca tcagatgcgc caacaatacc tgttgccaaa
      361 ggcgtccatg cagttaacgt accgctatat acccctttca ctggttgaat ggtattatta
      421 acaacgatag gcttggtcaa agtaagatta gctgacgctt cagtaccctg gcttactaaa
      481 gttcccgcct gagaagcaaa agctgtcccc gccagcatga ttgctaacaa tgttttcttc
      541 attatatttt ctccatgatt ttcatttaca ctgcaaaatg cagtttcttt ctttgcattt
      601 acgtgctatc gcgaaaatgc ttccaccaaa taatggaaac atcttcacaa aaataaatta
      661 tgagtttctt aatttagaaa ctataaacat gtcgccgcta tctggtataa ccctgatgca
      721 cttttcttcg caggaagttg gtattttgcg atacaggatt gatctttaga ataaccccat
      781 ttcacattaa actcgccttc cgcactcaag ccagaaacat aagcaatacc atcttcacct
      841 acaatgccgg ccatattttc agtgtctttg ctataactaa tcacagcgcc gaaggggatg
      901 ggtttaccct ctttctgttt taaggttact aatcctttat ccccgct
//
LOCUS       DAEBXN010000360          923 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.370, whole genome shotgun sequence.
ACCESSION   DAEBXN010000360 DAEBXN010000000
VERSION     DAEBXN010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..923
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.370"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            1..200
                     /locus_tag="KJL99_004655"
                     /pseudo
     CDS             1..200
                     /locus_tag="KJL99_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415867.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            300..>923
                     /locus_tag="KJL99_004656"
     CDS             300..>923
                     /locus_tag="KJL99_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HBD4439069.1"
                     /translation="MSTKPLFLLRKAKKSSGEPDVVLWASDDFESTSTTLDYLLVKSG
                     KKLSNYFKAVATNFPVVNDLPPEGEIDFTWSERYQLSKDSMTWELKPGAAPDDVHHQD
                     NAQETKELAGGQEENAQADAHEDCQDCEVSVATLRFTQRLLHIFTYAAGDLKYLHHAT
                     REQRKHITALEMDQENSYVQNLLLAIRGMAEPTTLDNAALLRLTDAIK"
BASE COUNT          293 a          217 c          205 g          208 t
ORIGIN      
        1 attggctacg taattaaaac agaaaataaa gaaaccccat tcactgtagt tgatatcgac
       61 ggtctatcag gaaactttaa aactcttgtt gaaggtgcca caaaaatgtg cctagtttac
      121 atcggaaata atctgcccgc agaaaaaaag ccgaatttct ggcaaatctg attgcaatga
      181 aattaaaagg tgaaatctga aaaaaagaaa gcctgcacag tgtgcaggcc tgagtgaaga
      241 acctgggaca tttattcatc actcgcagta attttaatct gagttgaggt taaaaaacaa
      301 tgagcacaaa accactcttc ctgttacgga aagcgaaaaa atcatccggt gaacctgacg
      361 tcgtcctgtg ggcaagcgac gattttgaat cgaccagcac cactctggac tatctgctcg
      421 ttaagtcagg taaaaaactg agcaactatt ttaaagctgt tgccacaaat tttcctgtcg
      481 ttaatgacct tccccctgaa ggtgagatcg attttacctg gagtgaacgc tatcaactca
      541 gcaaagactc catgacatgg gaactaaaac cgggagcagc gccagacgac gttcaccacc
      601 aggataatgc tcaagaaacc aaagaactgg cgggaggcca ggaagaaaac gcgcaggcag
      661 acgcccacga ggattgccag gattgcgaag tctctgtagc cactttgcgg ttcacacagc
      721 gtcttctgca catttttacg tatgctgccg gggatctgaa atacctgcat catgccaccc
      781 gtgaacaacg caaacacatt actgctcttg agatggatca ggaaaacagc tatgtccaga
      841 atctgctgtt ggccatacgc ggcatggcag aaccgacaac tctggataat gccgccctgc
      901 tccgcctgac tgatgcaatt aag
//
LOCUS       DAEBXN010000361          915 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.371, whole genome shotgun sequence.
ACCESSION   DAEBXN010000361 DAEBXN010000000
VERSION     DAEBXN010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..915
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.371"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(38..226)
                     /locus_tag="KJL99_004657"
     CDS             complement(38..226)
                     /locus_tag="KJL99_004657"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4439070.1"
                     /translation="MTNEELQAIALSMLERQREKVHQQRESLLNPSPFTRPKRLELEA
                     FLDKYPRRLKSGKTRPPG"
BASE COUNT          244 a          176 c          211 g          284 t
ORIGIN      
        1 atccgtttta ttgaatcgga gaacaaactg tgagctatca gcctggtggt ctggttttgc
       61 ctgatttaag ccgccgtggg tatttgtcca agaatgcttc aagctccaat cgttttggcc
      121 tggtgaatgg agatgggtta agcaaagact ctcgttgctg gtgtactttt tcgcgctggc
      181 gctcaagcat ggataatgcg attgcctgta gttcttcgtt agtcattgtc tgcacctgga
      241 aagcaattta ccggagtacc tcacactacg gcaaaaatgt tttattgttg caacttgcgt
      301 aattattcag gtcagtttgg aacgcactgt ataaacataa ataacctgat actgttgtgt
      361 gttttcaaag aaaacacagg tatttttcac ataaaccttc cccttttcat attcgaccac
      421 ttaatcaaaa aacggtttta tacttgttgc ccccactggg gttaaatgtg agacatgtcg
      481 cactttgtgc catgtgtcct cgctgatttt aggcgcggtt cattcattct ttctgttggc
      541 ataaagtatg gtatcggttg ggcgaatgaa agggactcgg gtcatggtca gacaatacct
      601 gaggcgttaa ttcgttgaaa gagattgtga atgggggaat aactgagtct taactgctgt
      661 gcgatagccc ggacagatag ttgctcgtcg aatctgagac gtaatgattc tggcaaataa
      721 gtgcgaaagc cggtgatatc tagtgttgtt tgagagttca tcgttcgcgt ccattaatac
      781 agatggacgc gattgttgcc agacaggata attttcacaa gacatcgcag atagagtact
      841 tacgataatt ttttatttta tacaacagat gcccacgaaa agaattattt tcaatagaca
      901 cctgcattac acaaa
//
LOCUS       DAEBXN010000362          911 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.372, whole genome shotgun sequence.
ACCESSION   DAEBXN010000362 DAEBXN010000000
VERSION     DAEBXN010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..911
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.372"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(<1..139)
                     /gene="ospE1"
                     /locus_tag="KJL99_004658"
     CDS             complement(<1..139)
                     /gene="ospE1"
                     /locus_tag="KJL99_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspE1"
                     /protein_id="HBD4439071.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IY"
     gene            complement(248..378)
                     /locus_tag="KJL99_004659"
                     /pseudo
     CDS             complement(248..378)
                     /locus_tag="KJL99_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612303.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(376..471)
                     /locus_tag="KJL99_004660"
                     /pseudo
     CDS             complement(376..471)
                     /locus_tag="KJL99_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365624.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          264 a          160 c          212 g          275 t
ORIGIN      
        1 cgtatatagc tgctttttta ttgaaaacag tatatccatc tgtagtaaca gtagaagata
       61 ataatacagg atttgatact tcaaggatga tattttcttg tttttgtgga agacaaggga
      121 atatagtttg tgtaagcata ttacttcctt atgttatatt ttggtaaaaa atttattttt
      181 ttacattata gtcggctaaa tgaagtttac tgtaaaaaaa cgatactttt tcgggtggcg
      241 cttccaatca gtagaatatg tgtttttcaa tgaaggagcc gatgactttt cgtggatttc
      301 cacggaacgc gagaagcaga ttgttttacg tgccagccgc ttgatgcggg tgcggagggt
      361 caggttgtta cgttcaagat atagcatgtg ctggatgaag atcccttttc tggtcacctg
      421 tttatctttc gtggccgacg gggtgacact gttaaaatgt aatgaacgca tcccacacaa
      481 ttaaacacgg agtagtatga tggttaaaag ggtgaaactg gcaggtgtgg agcctcgcag
      541 ctttccggca tcatactgcc caatacggga tgcataaagc gtcctcagac tggagactcc
      601 acaaatgaaa tatacaccgg ttggcgttga tatggcaaaa catgtcattc agattcactt
      661 catcaatgag cacatgtcag cgccagtgat ataagacggt aatggggatg cggacgattt
      721 cctggactct cagaagatga aacatgttta ctgaagaaca aaaaattcgc gctattgagc
      781 tttatttcaa atatggtaag aagcttgagg tgtactggca atagcggaca ccaccatttg
      841 ttctttttct aagcagtcat ctgataatat tttatcctga aggctgccgg ggagatattc
      901 cccagacgag a
//
LOCUS       DAEBXN010000363          905 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.373, whole genome shotgun sequence.
ACCESSION   DAEBXN010000363 DAEBXN010000000
VERSION     DAEBXN010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..905
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.373"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(<1..226)
                     /locus_tag="KJL99_004661"
     CDS             complement(<1..226)
                     /locus_tag="KJL99_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBD4439072.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQGEYDSQWATICSIAPKIGCTP
                     ETLRVWGRQHERDTGGGDGGLTTAERQRLKE"
     gene            complement(282..533)
                     /locus_tag="KJL99_004662"
                     /pseudo
     CDS             complement(282..533)
                     /locus_tag="KJL99_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
     gene            complement(554..>905)
                     /locus_tag="KJL99_004663"
     CDS             complement(554..>905)
                     /locus_tag="KJL99_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBD4439073.1"
                     /translation="TVHKCTFVFSPQVIKQRQFSGPHYKVDSIRESWTSILKRAGLRH
                     RKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQMVFNVYGAWMKDNNHEQIELLNRR
                     LSESVPCMPHKKAG"
BASE COUNT          198 a          230 c          211 g          266 t
ORIGIN      
        1 gctctttcag acgctgacgt tcagcggtgg tgagccctcc atcaccgccc ccggtatccc
       61 gctcatgctg gcgaccccag acacgcagag tctccggcgt acagccaatc tttggagcaa
      121 tggaacaaat tgtcgcccat tgtgagtcat attcgccctg actttccaga accatacgga
      181 ctgcccgttg acggacttcg ggggaaaaac gagtattttt agtcatcctg tttacctctt
      241 tctcaggaag tttagtctcc aggattcccg gggcggttca cgctttgcag gtaagtcagt
      301 aatgtttctt gctgatcgcg ccagacgttc tgcacatcaa cattttcatt actgagcagt
      361 attttaactt tgctgacctg cacgccgttg tcgatccagc gtttgatctc gcggatccgg
      421 tcaatatcgg catcgttgaa cagccgatga ccgccgtctg tccgttgcgg tttcagcaat
      481 ccgtaacgcc tctgccacgc gcgtaacgtg acagggatta aaggtaaacc aattgatttt
      541 gcaagttttt attttacccc gctttcttat ggggcatgca tgggacactt tcagatagtc
      601 ttctgttaag aagttctatc tgttcgtgat tgttgtcttt catccatgct ccgtaaacat
      661 tgaataccat ttgtgcgttt gtgtggccca tctggcttgc gataaaacta ggattagctc
      721 ctgcggcaag tgaccagcat gcataggtat gcctggattg atacgatttt ctgtgtctca
      781 gacctgcgcg ttttaagata cttgtccatg actccctgat ggagtcaacc ttatagtgcg
      841 gtccggaaaa ctgccgctgt tttattacct gaggactaaa aacaaaagtg catttatgca
      901 cagta
//
LOCUS       DAEBXN010000364          883 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.374, whole genome shotgun sequence.
ACCESSION   DAEBXN010000364 DAEBXN010000000
VERSION     DAEBXN010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..883
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.374"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..626
                     /locus_tag="KJL99_004664"
     rRNA            <1..626
                     /locus_tag="KJL99_004664"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            712..787
                     /locus_tag="KJL99_004665"
     tRNA            712..787
                     /locus_tag="KJL99_004665"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:746..748,aa:Glu,seq:ttc)
BASE COUNT          220 a          209 c          251 g          203 t
ORIGIN      
        1 gaattgacgg gggcccgcac aagcggtgga gcatgtggtt taattcgatg caacgcgaag
       61 aaccttacct ggtcttgaca tccacggaag ttttcagaga tgagaatgtg ccttcgggaa
      121 ccgtgagaca ggtgctgcat ggctgtcgtc agctcgtgtt gtgaaatgtt gggttaagtc
      181 ccgcaacgag cgcaaccctt atcctttgtt gccagcggtc cggccgggaa ctcaaaggag
      241 actgccagtg ataaactgga ggaaggtggg gatgacgtca agtcatcatg gcccttacga
      301 ccagggctac acacgtgcta caatggcgca tacaaagaga agcgacctcg cgagagcaag
      361 cggacctcat aaagtgcgtc gtagtccgga ttggagtctg caactcgact ccatgaagtc
      421 ggaatcgcta gtaatcgtgg atcagaatgc cacggtgaat acgttcccgg gccttgtaca
      481 caccgcccgt cacaccatgg gagtgggttg caaaagaagt aggtagctta accttcggga
      541 gggcgcttac cactttgtga ttcatgactg gggtgaagtc gtaacaaggt aaccgtaggg
      601 gaacctgcgg ttggatcacc tccttacctt aaagaagcgt tctttgtagt gctcacacag
      661 attgtctgat agaaagtgaa aagcaaggcg tcttgcgaag cagactgata cgtccccttc
      721 gtctagaggc ccaggacacc gccctttcac ggcggtaaca ggggttcgaa tcccctaggg
      781 gacgccactt gctggtttgt gagtgaaagt cacctgcctt gatatctcaa aactcatctt
      841 cgggtgatgt ttgagatatt tgctctttaa aaatctggat caa
//
LOCUS       DAEBXN010000365          881 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.375, whole genome shotgun sequence.
ACCESSION   DAEBXN010000365 DAEBXN010000000
VERSION     DAEBXN010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..881
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.375"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..>881
                     /locus_tag="KJL99_004666"
     CDS             <1..>881
                     /locus_tag="KJL99_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBD4439074.1"
                     /translation="SITTNRYASGYATLTEAVSAQDERNRKRDKNSHDGSTISLSQPL
                     GNIGNLNFNTTRYNSSRGTGNTRSTSLSYSTVWRGITFSINWAKNDLLTSHKWKVDRK
                     LSVGISVPLSLGDENQIYASSQMSRSGEQGNNYQVSLSGQNSGGVWWDVATNITNAHQ
                     SQPKSTMNIVQVGKNGSYGQFSSHYSSSENMKQLGANLSGGILITRDGLTFGQNVDGT
                     LALIEAPGATGVNVNGWPGLSTDFRGYAILPVQPYRRDDVILDEKTIGKNYDLPQTSQ
                     LVVPTAGAVVPATLAVK"
BASE COUNT          275 a          169 c          226 g          211 t
ORIGIN      
        1 gtctataacc acgaaccgtt atgcatcagg ttatgccaca ctgactgaag cagtaagtgc
       61 acaggatgaa cggaacagaa aaagagataa gaatagtcac gatggctcta cgatatcctt
      121 atcccagccc cttggtaata tcgggaattt gaacttcaat accacgaggt ataattcttc
      181 ccggggcaca ggaaacacgc ggtctaccag cctgagttat agtacggtat ggagaggcat
      241 aacattctcc attaactggg caaaaaacga cttgctgaca tcgcataaat ggaaagtaga
      301 tcggaaatta agcgtgggta tttcagtgcc attatcgctg ggtgacgaaa atcaaattta
      361 tgcgtcttca cagatgagtc gttcgggaga gcagggtaat aactatcagg tttcattatc
      421 aggtcagaat agtggtggtg tatggtggga tgtagcgaca aatataacta acgctcatca
      481 gtcacaaccg aagagcacga tgaacattgt gcaagttggc aaaaatggat catatggtca
      541 gtttagtagc cattacagct ccagcgaaaa catgaaacag ctaggtgcaa atttaagcgg
      601 tgggatactt attacaaggg atggacttac atttggtcaa aatgtggatg ggacattagc
      661 ccttatagag gcacccggag caacgggggt taacgttaat ggctggccgg gactgagtac
      721 tgattttcgt gggtatgcaa tactgccagt gcagccatac cgaagagacg atgttattct
      781 ggatgaaaaa acgataggta agaattacga cttaccacaa actagtcaat tggttgtacc
      841 gacagctgga gccgttgtgc ctgcgacatt ggcagtgaag a
//
LOCUS       DAEBXN010000366          870 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.376, whole genome shotgun sequence.
ACCESSION   DAEBXN010000366 DAEBXN010000000
VERSION     DAEBXN010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..870
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.376"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..>870
                     /locus_tag="KJL99_004667"
     CDS             <1..>870
                     /locus_tag="KJL99_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001689732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HBD4439075.1"
                     /translation="LSSAAVKTAGVLISFQAVLSAYQRVMEVGLQRASAERSIDFVFS
                     KDANQVKDFTKSLAQTTGLDIAELQSQFAGFGASARESLGIQGSEELFRNMIGYSRLM
                     GRSEEEIKRALTALSQMAGKGQIMAEELKGQLAEAVPGMVQVFAKATGKTEQELFDAM
                     KKGALKSADTLQKVTQELNKQITAKGGWKAISESTQAQLGNLKNSWNTTLDSIFRGSE
                     NGLQDFTRSLTNLLNALGGSGKSLGESLGSLMTSMSHGVDSLTDISYRVRAFFDEVTL
                     AYRPLNDTQRKIV"
BASE COUNT          263 a          153 c          209 g          245 t
ORIGIN      
        1 tttaagttca gcagcagtta aaacagcagg tgtacttatc agctttcagg ctgtactatc
       61 cgcttatcag cgagtaatgg aagttggcct tcaacgtgca agtgctgaac gttcaattga
      121 tttcgtattt tctaaagatg caaatcaagt aaaagatttc acaaagtcat tagctcaaac
      181 aacgggttta gatatcgctg aactgcaatc acagtttgct ggtttcggtg cttcagctag
      241 agagtctcta ggcattcaag gttctgaaga actttttaga aatatgattg gctattctcg
      301 tctgatgggg cgaagtgaag aagagattaa gcgagcactg acagcattat cacagatggc
      361 tggtaagggt cagattatgg ctgaagagct taaggggcaa ctagctgaag ctgttcctgg
      421 tatggttcag gtattcgcta aagcaaccgg aaagactgaa caggaattat ttgatgctat
      481 gaagaagggg gctttaaaat ctgccgatac tcttcagaag gtcactcagg aactgaataa
      541 acaaatcacc gctaaaggtg gctggaaagc tatttcagaa agtacacagg cacaactagg
      601 gaacttgaaa aactcttgga ataccacgct tgatagtatc ttcagaggtt ctgaaaatgg
      661 tctgcaagat tttacaagaa gcttaaccaa tcttttaaac gctcttggtg gaagtggtaa
      721 gagtcttggt gaatctcttg gtagtctcat gacttctatg tcacatggtg tcgattcatt
      781 gaccgatatt agttacaggg taagggcgtt ctttgatgag gtgacattag cttaccgtcc
      841 actaaatgac actcagagga aaattgttga
//
LOCUS       DAEBXN010000367          862 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.377, whole genome shotgun sequence.
ACCESSION   DAEBXN010000367 DAEBXN010000000
VERSION     DAEBXN010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..862
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.377"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            112..459
                     /locus_tag="KJL99_004668"
                     /pseudo
     CDS             112..459
                     /locus_tag="KJL99_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
BASE COUNT          242 a          211 c          194 g          215 t
ORIGIN      
        1 gcttatcgcc ctggcgagac gacgctgcga cgttctgttc gccatgatgc gcgacgggac
       61 tttttatacc ccgcaggggt cataacatgc ttgacaactt aataggggca ctcccccgct
      121 aaagtgttgc ccgaaaactg gtggcaacat ccagctgcat taggtgcaac tgacagtgat
      181 atcgaaatca tcaaacgtca gtggggtgcg ttttacggta cggatctgga gttgcaactg
      241 cgccgccgag gtatcgatac aatagtgtta tgtgggatct cgaccaatat cggtgttgaa
      301 tccaccgccc gcaatgcctg ggaacttggc tttaatctgg tgattgccga agacgcctgt
      361 agtgccgcca gcgccgagca acacaataac agcattaatc atatctaccc gcgcatcgcc
      421 cgtgtgcgta gcgtggaaga gatccttcac gcgttatgat ttacatcggt ctaccgcaat
      481 ggtcacatcc taaatgggtg cggttgggga tcaccagcct tgaagagtat gcccgccact
      541 ttaactgcgt gatacgggat ttttaaaaat cactaaagaa cgcccaagag catgtgtttt
      601 ctttagaata atcaaagcgt tggattgcca tgtcatatga aaatcggcaa agttcatatg
      661 taacaaagtg tcccaatcat gccccaaaat gccccacaac aaatattttt gccccatgca
      721 tgccccaaaa aatcactttc cctcactctt gacactgttt atccatacag ttaaaaataa
      781 tactgtataa aaacacagtg cagagggatt tttatgcgtt ttgaaatctg catagccaaa
      841 gagaaaatga ctaaaatgcc aa
//
LOCUS       DAEBXN010000368          843 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.378, whole genome shotgun sequence.
ACCESSION   DAEBXN010000368 DAEBXN010000000
VERSION     DAEBXN010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..843
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.378"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(59..135)
                     /locus_tag="KJL99_004669"
     tRNA            complement(59..135)
                     /locus_tag="KJL99_004669"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(99..101),aa:Arg,seq:tct)
     gene            complement(137..213)
                     /locus_tag="KJL99_004670"
     tRNA            complement(137..213)
                     /locus_tag="KJL99_004670"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(177..179),aa:Ile,seq:cat)
     gene            complement(403..>843)
                     /locus_tag="KJL99_004671"
     CDS             complement(403..>843)
                     /locus_tag="KJL99_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HBD4439076.1"
                     /translation="GGKTGNMFNLMLTSKKLTKTAINEALRRMKKAGLNKSELEAFLR
                     DMINGKQKSWLAHCTDAEALCIDRVISEVLAEHPGLISVLRQRYEGRGMTKCKMAELL
                     NDAHPKWSLRTCERRIEHWLKVAEFILYKPMVMAFGIEKKVIAF"
BASE COUNT          209 a          234 c          167 g          233 t
ORIGIN      
        1 gaactggtga cctactgatt acaagtcagt tgctctgcct gctgagctaa gtcggcgctg
       61 gcccaccacc gaggactcga acctcgcacc gtcaacttag aaggttgatg ctctatccgg
      121 atgagctagt ggtggttggt ggcccttgct ggacttgaac cagcgacctg gcgattatga
      181 gtcgctcgct ctgaccaact gagctaaagg gccggaggca gaataataac catatgtcat
      241 cacatctgca aactcatctg atcaccagca cgtttaacgt cctgtgccgt ttttcaggta
      301 taaaaaaaac cgcataaagc gggctctttc aaatgtccat ctctgctatt cgcctcgcgg
      361 tacagctttg cgaagcgtac aagaattgaa gcagttttta cgtcaaaaag caataacttt
      421 tttctctata ccaaaagcca taaccattgg tttgtacaaa ataaattctg ccacctttag
      481 ccaatgctca atgcgtcttt cacaggttct taaactccat ttcgggtgtg catcattcag
      541 cagttcagcc attttgcatt tggtcatccc ccgcccctca taccgttgcc gaaggacgct
      601 aatcaatcct ggatgctctg ccagcacctc acttatgacc cgatcaatac ataacgcctc
      661 tgcatcagta caatgcgcca gccagctctt ttgcttaccg ttaatcatat cccgcaaaaa
      721 agcctcaagt tcagacttgt tcagacctgc ttttttcatc ctccggagcg cctcgttaat
      781 tgccgttttt gtcagctttt tagaggtcaa catcaggtta aacatattcc ccgtcttacc
      841 gcc
//
LOCUS       DAEBXN010000369          833 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.379, whole genome shotgun sequence.
ACCESSION   DAEBXN010000369 DAEBXN010000000
VERSION     DAEBXN010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..833
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.379"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            1..664
                     /locus_tag="KJL99_004672"
                     /pseudo
     CDS             1..664
                     /locus_tag="KJL99_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001689732.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
BASE COUNT          252 a          163 c          177 g          241 t
ORIGIN      
        1 gtttatctga tgggttaatt gatggtctga agatgcttgc tgtggctgtt actgctcaaa
       61 aggctgcaaa tgtagcagga ggcttgttaa accttgcaaa cgctgttact tcattcggtg
      121 acgctgttaa taaagctaaa gctggcaagg ctggttcttc aggtggtgcg ctaaataaag
      181 tcggtagagt tgtcctttat gctgagttgg taaattttgc tcttgataat ttaaaccctg
      241 aagcagctaa gaatccgaac ttacatcaat caggtattgg cacagaaaca aacttcaacc
      301 ctgaatacaa aggtgattca tctaagccaa tagttctatc gctgactggt ttaatggtgc
      361 aactaataaa attgctaact tccaagaacc gatgaataac ttcgtaaata acacacaatc
      421 aactccatca gctatcacag gtgacatggt taatagcaat gctcaattag ctatggctgt
      481 atcagagtta ggaagaaagg tagacgcttt aacagcagaa actaagaagc ctttatcagt
      541 taatatcact ggtgaagcaa ctgttagacc ggatgcacca tctttcttta gcttcaatca
      601 atcagctaat gaccaattca cgcaggatat gctcttaagt tcaagctatc cagaggaaga
      661 atgatttctt atatatccgt gtgctacgtg aaatattctg aacagatata cccactgaaa
      721 aatagtgaac cgccccgggt ttcctggaga gtgttttatc tgtgaactca ggctgccaga
      781 tcatcgtttc cgatggaagc ataataagct ttttctgctt ctgccggagg gat
//
LOCUS       DAEBXN010000370          831 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.380, whole genome shotgun sequence.
ACCESSION   DAEBXN010000370 DAEBXN010000000
VERSION     DAEBXN010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..831
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.380"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..326
                     /locus_tag="KJL99_004673"
     CDS             <1..326
                     /locus_tag="KJL99_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HBD4439077.1"
                     /translation="SETFSVVMTKDPGAACTEQFNILGSFFTTDILSDYSHLDMGNGL
                     LLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANPAETVVEGPFSKDVIL
                     KITYN"
     gene            complement(369..569)
                     /gene="glgS"
                     /locus_tag="KJL99_004674"
     CDS             complement(369..569)
                     /gene="glgS"
                     /locus_tag="KJL99_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HBD4439078.1"
                     /translation="MEHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
BASE COUNT          252 a          184 c          154 g          241 t
ORIGIN      
        1 ttagtgagac gtttagtgta gtcatgacaa aagatcctgg tgcggcctgt actgagcaat
       61 tcaatatttt agggagcttt ttcactacgg atattttgag tgattatagc catctggata
      121 tgggtaatgg actgctattg aagatatttc ataaagatgg aacagcaacg gaatttaacc
      181 gcttctcaca atttgcttct ttttcatcgt ctagtgcacc ttcggtcacc gcaccattca
      241 gggcagaact tagtgcgaac ccggcagaga ctgttgttga gggaccgttt agtaaagacg
      301 taatcctcaa aatcacctat aactagcatc taatacaaac actaaaacgg gccatcaggc
      361 ccgttatatc agtgctctaa ctccagctct cttgcctgca tcatctgttg gcaataccag
      421 gcataacgtg cgcaaaacca agttctatgt tcttcatcca tgttaccagt aacagccaga
      481 atatcgaccg ggcgaccttc tgcgtgcatt ctggcaaaac tacgcgccag gaaatcgaaa
      541 ttattcaaag aattaagact atgctccata agcacatcct ccggttatcg catacctttt
      601 aaggccatat gctttttctg atttcagtat tccccaatca gtgtagtgcc tttaaacata
      661 acgtgcgtaa atatattgtc cccgatcaca ctttttagtg aaaaataaaa aaccatatta
      721 aaatactttc tcttccagaa cttctctaac atgtgttatt attaacaaag acaaatgtaa
      781 gcagggaaac tattccaagg taaatattgc gtcactaaat ggacattgga g
//
LOCUS       DAEBXN010000371          828 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.381, whole genome shotgun sequence.
ACCESSION   DAEBXN010000371 DAEBXN010000000
VERSION     DAEBXN010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..828
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.381"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            211..684
                     /locus_tag="KJL99_004675"
     CDS             211..684
                     /locus_tag="KJL99_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBD4439079.1"
                     /translation="MSWLISQCAHQCTDNKKTETDAIYDKVRSSYLLSCILKKNKNVG
                     LILHAPSFVSVSEKIARIVMANYSRNWSNSELASAVLMSESSLKRRMYKEVGSISTFV
                     HKIKLTEAIRKLRRTNTPISVISSELGYSSPSYFSKVFFKYLKTYPQNIRKKNGR"
BASE COUNT          257 a          172 c          183 g          216 t
ORIGIN      
        1 gagcaggtac cggcatccac gttacagacg gtcccggcaa aagtgcctga cctgacccct
       61 attagcggtt accagcgccc caccagaccc ggtgacatca ccccccggat catggacgac
      121 tgggggcagt aatcatgccg ggtctggctg gctaacaccc cattataagg ggtacgaata
      181 aagtaacggg ggggagatga accctatgca atgtcctggt taatatctca gtgtgctcat
      241 cagtgcactg ataataaaaa gacggagaca gatgccattt acgataaagt tcggagttct
      301 tatttgcttt catgtattct gaaaaaaaac aaaaatgttg ggcttattct tcatgcacca
      361 tcatttgttt ctgtgtctga aaaaatcgcc agaattgtga tggcaaatta ctcccgtaac
      421 tggagtaatt ccgagttggc cagtgcggtt ttaatgagtg aatcttcttt aaaaagaaga
      481 atgtacaaag aggtcggttc tataagtaca tttgtacata aaataaagct tacagaagct
      541 atcaggaaat tacgaaggac aaatactccc atttctgtca tatcatctga gttaggttac
      601 agttcaccat cgtatttttc taaagtcttc tttaaatatt taaagacata ccctcaaaac
      661 ataaggaaga aaaatggtcg gtaatgtcac caattaggag gacttgcaga gccgcttttt
      721 tacgatactg agatcaaaca gacataatct atgcggagat ttacagcagg gcatggttca
      781 caaatctgac agcgatgaat tatcggcact gcgggcagaa aatgctcg
//
LOCUS       DAEBXN010000372          818 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.382, whole genome shotgun sequence.
ACCESSION   DAEBXN010000372 DAEBXN010000000
VERSION     DAEBXN010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..818
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.382"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            1..>818
                     /locus_tag="KJL99_004676"
     CDS             1..>818
                     /locus_tag="KJL99_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBD4439080.1"
                     /translation="MFKANHVPVLMYHHVSHCPGLVTLSPETFRKQMKWLAENNWKTL
                     SSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLNEFNLKAHIFLITSFIGNGP
                     VRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTHTRWDKKF
                     TSREEQCKHLRQDLLSGREYLKKMTGKCSKHLCWPEGYYNKDYIQIAEELGFHYLYTT
                     ERRMNAPAKGTTRIGRISTKERENCVWLKRRLFYYTTPFFSSLLALHKGPRLPD"
BASE COUNT          262 a          145 c          175 g          236 t
ORIGIN      
        1 ttgtttaaag caaatcatgt tccggtatta atgtatcatc atgtcagcca ttgcccagga
       61 ctggttacac tttctcccga gacattccgg aaacaaatga aatggttggc tgaaaacaac
      121 tggaaaacac tgtcatcaga tgaactggag tttttttatc ggggcggaaa acttccccgt
      181 aaaagcgtta tgttgacatt tgatgatgga taccttgaca actggtttca ggtatatcct
      241 ttattaaacg agtttaatct caaggcacat atttttttaa ttaccagttt tattggaaat
      301 ggtccggttc gtcactctcc tggaaaggaa tattctcatc gcgattgcga acatcaaata
      361 gcaaccggga atgctgataa tgttatgctt cgctggtctg aagtgaacga aatgttgcaa
      421 tctggtctgg tcgaatttca tgttcataca catactcata cacgttggga taaaaagttc
      481 acttcacgag aagaacagtg taaacatctt cggcaggact tattgagcgg gagagaatat
      541 ttaaaaaaaa tgacaggaaa atgcagtaaa catttgtgtt ggccggaagg ttattataac
      601 aaggattata ttcagatcgc tgaagaactt ggattccatt atttatatac aacagaaaga
      661 agaatgaatg ctcctgctaa aggaactaca cggataggaa gaataagcac caaggaacgg
      721 gaaaattgcg tatggctgaa aagacgactt ttttattata caacaccgtt tttttcatcg
      781 ttgctggccc ttcataaggg gccaagatta ccggatga
//
LOCUS       DAEBXN010000373          816 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.383, whole genome shotgun sequence.
ACCESSION   DAEBXN010000373 DAEBXN010000000
VERSION     DAEBXN010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..816
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.383"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            10..687
                     /locus_tag="KJL99_004677"
     CDS             10..687
                     /locus_tag="KJL99_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBD4439081.1"
                     /translation="MSIIFNGHYRMKHRTWITEALRLHFEEHLPRVVAGRRLGVPKST
                     VCSMFVRFRRAGLSWPLPSGMSEQELDACLYGQFSTVPVVRPESTVISEAPVVKKRPR
                     RPNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQYRKGGLLPSGKNMPA
                     LLPVTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLRLKGKLTPALLQTLIR
                     EIKGSSH"
     gene            687..>816
                     /locus_tag="KJL99_004678"
     CDS             687..>816
                     /locus_tag="KJL99_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HBD4439082.1"
                     /translation="MISLPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHL"
BASE COUNT          199 a          222 c          205 g          190 t
ORIGIN      
        1 acaaaaataa tgtccatcat ttttaatgga cactatcgta tgaaacaccg gacctggatc
       61 actgaagctt tacgtcttca ctttgaagaa catttacccc gggttgtggc cgggcgtcgc
      121 ctgggtgtac caaaatcaac agtttgtagt atgtttgtgc gctttcggag agctggcctt
      181 tcgtggcctt tgccctcagg catgtcggag caggaacttg atgcctgcct ttacggacaa
      241 ttttccacgg taccagtcgt acgtcctgaa agcaccgtta tatccgaagc ccccgtggta
      301 aaaaaacgtc cccggcggcc caacttccct tatgagttta aaatcgcctt agtggagcag
      361 tcactgcagc ccggagcctg tgtggcgcag atcgcccggg aaaacggaat caacgataac
      421 ctgctcttca actggcgcca tcaataccgg aaaggtggcc tgctgccttc cggaaaaaat
      481 atgccggcac tgcttcccgt gacgttaacg ccggagccgg ataataaaat cccggctccg
      541 gcacaggaac cagagcagat aaatacaccg tccgacagtc tgtgttgtga gctggttctg
      601 ccggccggaa ctctcaggct taaaggtaaa ctgacgccgg cgttattaca gacacttatc
      661 cgcgaaataa aagggagcag ccactgatga tatctctccc tgcaggttcg cgtatctggc
      721 tggttgccgg tatcaccgat atgcgaaatg gctttaacgg cctggcctca aaagttcaga
      781 acgtcctgaa ggatgacccg ttctccggac acctgt
//
LOCUS       DAEBXN010000374          813 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.384, whole genome shotgun sequence.
ACCESSION   DAEBXN010000374 DAEBXN010000000
VERSION     DAEBXN010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..813
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.384"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..794
                     /locus_tag="KJL99_004679"
     CDS             <1..794
                     /locus_tag="KJL99_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4439083.1"
                     /translation="IDSISHFWTGLCDEYARRRNRKRGLEFSDWAWLKQEWRRFTDRF
                     VNSQAHIIMCGRAGYEYDFFEGDDGKRQLAKTGIKMKAETETGYEPSIRIQMEKQMNI
                     ETGQVWRTARILKDRSTRIDGQVFSNPTFKNFLPHIESLNLGGEHPGIDTSRDNSELF
                     ANDGTPTWLKEKRAKEIALDEIIELLNKHHGGTSNDAKRAKADLLEKTFTSRSWERIK
                     GMDWPTIKAGRNALWIELEGVEYAFPDPQTQNETQQAGYDENIPV"
BASE COUNT          265 a          185 c          195 g          168 t
ORIGIN      
        1 tcatcgactc catcagtcac ttctggaccg gattatgcga cgagtatgcc agacgccgaa
       61 accgtaaacg aggactggaa ttttcagact gggcgtggct caaacaggag tggcgacgct
      121 ttaccgatcg atttgttaac agtcaggcgc acattattat gtgcgggcgt gcaggatatg
      181 agtatgactt tttcgaaggt gatgatggca aacgccagtt ggcaaaaacc ggtataaaaa
      241 tgaaagcaga aacagaaacc ggttatgaac cctccatccg gatccagatg gagaagcaaa
      301 tgaacataga aaccggtcag gtgtggcgca ccgccagaat actaaaagat cgctccaccc
      361 gtattgatgg acaggtattt tcaaacccaa catttaaaaa ctttcttcct catatcgagt
      421 ctcttaacct gggaggtgaa caccccggca tcgatacctc acgggataac agtgaattat
      481 ttgccaacga cggtacaccg acatggctaa aagaaaagcg tgcaaaagaa atcgcacttg
      541 atgaaattat cgagttgctc aacaagcacc atgggggcac cagtaacgac gccaaacgcg
      601 caaaagctga tttgctggaa aaaacattca cctcccgctc atgggaaaga atcaaaggta
      661 tggactggcc gacaatcaaa gctggtcgta atgcactatg gattgaactg gaaggcgttg
      721 aatatgcatt cccggatcca caaacacaaa acgaaacgca acaagcagga tacgatgaga
      781 atattccggt gtaaaaccaa gtgcattttg gga
//
LOCUS       DAEBXN010000375          800 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.385, whole genome shotgun sequence.
ACCESSION   DAEBXN010000375 DAEBXN010000000
VERSION     DAEBXN010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..800
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.385"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(21..692)
                     /gene="queE"
                     /locus_tag="KJL99_004680"
     CDS             complement(21..692)
                     /gene="queE"
                     /locus_tag="KJL99_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HBD4439084.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          201 a          209 c          176 g          214 t
ORIGIN      
        1 taacgcttat aaatgtgtaa tcaggcaata tttaaatatt tatgtgtttg catcgacaaa
       61 cgccaattac gcgcaataca ggtttcaatg cacaatcgtg tggcatcatc cttttggcta
      121 atcggctgca gtgcaatgac tcgcggttta tcatcggtca gcgttgccag tagttcatcc
      181 agtgcttcaa tatcgcgtac gcgccccact ggatgcttga tttcgttggc tcgctccagt
      241 gcctgtgaca acacttcata gccgccgcgc atgttcagct ttggcgatac ggtaacccag
      301 gtattcggtg tgcagcgtac ttcatgagtt ccactggttt cgatctggca gctaaaaccg
      361 ttcttttcga gcaggtcagt cagtggcagc aaatcatgaa tgcaaggctc accacccgta
      421 atcaccacat gccgcgcggt atatccctga cgaccaatga cagccagcaa gtcttcactg
      481 ctcgccgccc cccacttatc actctctttg gtcttcgcca gaatgctgaa aagggagact
      541 tcccgatcct caagcttttc ccaggtgtgt ttggtgtcgc accaggcaca gccaaccggg
      601 catccctgta aacgaataaa aatggcggga acgccggtaa agtaaccctc accttgcagg
      661 gtctggaaca tctcgttaat cgggtactgc atagcattct ctgtgaagtg gataattgtt
      721 aattattgca gatcctgccg caacaatcat gtcttattaa cattctgtta catgcagatt
      781 taaagaaaaa tacgaaacat
//
LOCUS       DAEBXN010000376          795 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.386, whole genome shotgun sequence.
ACCESSION   DAEBXN010000376 DAEBXN010000000
VERSION     DAEBXN010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.386"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            46..795
                     /locus_tag="KJL99_004681"
                     /pseudo
     CDS             46..795
                     /locus_tag="KJL99_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          233 a          166 c          197 g          199 t
ORIGIN      
        1 tactgcaccc attttgttgg acgatgaaat ggaatagccc ctgatatgtc aaagccaaaa
       61 tacccttttg aaaagcgcct tgaagtcgtg aatcactact tcactactga tgatggttac
      121 aggatcatct cggcacgttt tggtgtcccc cgaacccagg tcaggacatg ggttgccctc
      181 tatgaaaaac atggagaaaa aggcttaatt cccaaaccta aaggtgttag tgctgatcca
      241 gagttgcgta ttaaggtcgt gaaagctgtg attgagcagc acatgtccct taatcaggct
      301 gctgctcact ttatgcttgc tggtagtggt tctgtagcca ggtggctgaa ggtttatgaa
      361 gagcgcggag aagctggttt acgcgcgctc aagattggca ccaaaagaaa cattgcaata
      421 tcagttgatc cagaaaaagc ggcatcagca ttggagctgt caaaagaccg acgcattgag
      481 gatcttgaaa ggcaagttcg atttcttgaa acgcggcttg tgtatctaaa aaagctgaaa
      541 gccttagctc atcccacgaa aaagtgaaag tactcaacga gctaaggcag ttttatcctc
      601 ttgatgagct tctcagggct gcggagatac cgcgcagtac gttttattac catctaaagg
      661 ctctcagcaa gcctgacaag tatgcggacg ttaaaaagcg tattggtgag atttatcatg
      721 agaatagagg ccgatacgga taccgtaggg taacgctgtc tcttcatcga gaagggaaac
      781 agattaacca taaag
//
LOCUS       DAEBXN010000377          793 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.387, whole genome shotgun sequence.
ACCESSION   DAEBXN010000377 DAEBXN010000000
VERSION     DAEBXN010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..793
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.387"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            437..512
                     /locus_tag="KJL99_004682"
     tRNA            437..512
                     /locus_tag="KJL99_004682"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:470..472,aa:Ile,seq:cat)
BASE COUNT          242 a          130 c          180 g          241 t
ORIGIN      
        1 aatgtttaaa tctgtttttt aattatcgat gtggtctgga gaggcaactg tcaaaaatcg
       61 cgcatacgaa actaatgatt agcagaataa gatctatgaa agaatatatt gctatatgaa
      121 gtttattttt atggagtgaa aagtaacaga tatcataatg atgcgaaaac tcttcagcta
      181 atgtaataaa gttatattat tagatacaaa atcattacaa agtatcattg aatcgatgat
      241 aactatagat agtgctaatt attaacattt gcttgttgac tgcgagtatc gactttgcga
      301 atggaggcgg tgtcgggttt tatgtcgcgt gtaatcacga cccaaggtga atgggaacgc
      361 ataaaaatct gttatttaca tcataagatc atcatgttta ttgcgtggtt tataagtatt
      421 gttattgcat acttaaggcc ctttagctca gtggttagag caggcgactc ataatcgctt
      481 ggtcgctggt tcaagtccag caagggccac caaatcatca ttcatgtatc aggaagagtt
      541 aagggtacaa ggggtactcg atggcggtct atatttcatg actaacgcca tttcaaggaa
      601 gaagtgcggt gggtggtttc gaatgagggc tggtgttttg gtacgtggca cgccactgca
      661 atgtatttat tcatcagtac tcattcaaaa gtacctggaa aaaatgcccg ctatgccaag
      721 cgggcaaatt gatcctaccc acgtaatatg gacacaggcc taagcgaggt tctggttttc
      781 aaattgttcc gga
//
LOCUS       DAEBXN010000378          784 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.388, whole genome shotgun sequence.
ACCESSION   DAEBXN010000378 DAEBXN010000000
VERSION     DAEBXN010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..784
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.388"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(<1..668)
                     /locus_tag="KJL99_004683"
     CDS             complement(<1..668)
                     /locus_tag="KJL99_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol phosphorylceramide synthase"
                     /protein_id="HBD4439085.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSSTFEQRRLVHRLILATVMACCG
                     FFLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVR
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKLDQRRIK
                     IALP"
     gene            complement(658..>784)
                     /locus_tag="KJL99_004684"
     CDS             complement(658..>784)
                     /locus_tag="KJL99_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase family
                     protein"
                     /protein_id="HBD4439086.1"
                     /translation="MRVRSQGEMDSLVRNAGFDKCTQRIDVWGIFTVSMAVRRDN"
BASE COUNT          252 a          203 c          177 g          152 t
ORIGIN      
        1 tatggcagtg ctattttgat tcgacgttga tcaagtttct gataattcca acgacggtcg
       61 acgggcacca tccagtcaat caacatacct accgccagcc ctgtgatgac atcaataaaa
      121 tgatgctgcc atgtcgtcag cgtcgaaatg gcgatgagta aaaaccatcc gccacagact
      181 ttacgccacc tcacagccag atgctgacga aagtgacgcc aaagtagcca gcagagaata
      241 atatgcagcg aaggagactg gttataaggc agatcaaaca gttcaagttg cgaaaatagc
      301 catcctgtca ccccactcac ttcaggacgg ataaaactaa acttcagcgg gtagagaaaa
      361 aaaccgcagc aggccattac cgttgccaga ataagccggt ggacaagtcg gcgttgttcg
      421 aatgtcgagc tacaaacgaa cagcgaaaat ccatataaaa gatccagact ccagtaagga
      481 acaatagtcc agggaagaaa agggatcgcc gtttcccaac cgaatacctg actggggata
      541 tcatggctgt taaggtcctg aaccgcggtg aactgattaa gagatccata ggtgaagaag
      601 aaaaacgggg ccagcaacaa taaccagcca gcgccttgta gcaatacgtt ccgacgttca
      661 gttatcacga cgcaccgcca tcgaaaccgt aaaaatgccc cacacatcaa tccgttgagt
      721 gcatttatca aatccggcat tacgcacgag cgaatccatc tccccttgcg aacgcacgcg
      781 catt
//
LOCUS       DAEBXN010000379          762 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.389, whole genome shotgun sequence.
ACCESSION   DAEBXN010000379 DAEBXN010000000
VERSION     DAEBXN010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..762
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.389"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(<1..>762)
                     /gene="waaO"
                     /locus_tag="KJL99_004685"
                     /gene_synonym="rfaI"
     CDS             complement(<1..>762)
                     /gene="waaO"
                     /locus_tag="KJL99_004685"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HBD4439087.1"
                     /translation="KQYNSRINIYVINCDKLKSLPSTKNWTYATYFRFIIADYFYHKH
                     EKILYLDADIACKGSIKELLDYQFSTNEIAAVVAERDIEWWQNRASVLTTPQLASGYF
                     NAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLDQDVLNVLLNGKVKFISEKYN
                     TRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWADYPVSRSFLIAKAASPWSKED
                     LLKPVNSNQYRYCAKHKFKQKHYMAGIFNYLKYYK"
BASE COUNT          243 a          135 c          112 g          272 t
ORIGIN      
        1 ctttataata ctttaaataa ttaaaaatgc ctgccatata atgcttttgt ttaaatttat
       61 gttttgcaca ataccgatac tgattgctat ttacaggttt aagtaaatct tctttactcc
      121 acggagaagc tgctttggcg atcaaaaaac tacgtgaaac cggataatca gcccactcat
      181 gccagggttt tgtaggtccc acatagtgta taaacacggt gtcatcattg actggattat
      241 caactttgtc ttttaattca tagttaatac tatatcgggt attatatttc tccgaaataa
      301 acttcacttt accattcaat aatacattca gtacatcttg atcaaggtgg gtgattttac
      361 ttacccaatc tgggtcacgc aacatttcaa tagcttttga cgaaatgtta tttagattcc
      421 actcatcaat atttatcagt aaaaaaccag cattgaaata tccagaagct aactgtggtg
      481 tagttaatac cgaggctcga ttttgccacc attcaatatc tctttcagct acaactgcgg
      541 caatttcatt agtagaaaat tgataatcta agagttcttt aatactaccc ttgcaagcaa
      601 tatctgcatc aagatatagt attttttcat gtttatgata gaaataatct gcaattataa
      661 atcgaaaata tgttgcgtaa gtccagtttt tcgtgcttgg taatgacttc agcttatcac
      721 aattgataac ataaatatta attcttgaat tatattgttt tg
//
LOCUS       DAEBXN010000380          760 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.390, whole genome shotgun sequence.
ACCESSION   DAEBXN010000380 DAEBXN010000000
VERSION     DAEBXN010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..760
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.390"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..>760
                     /locus_tag="KJL99_004686"
     CDS             <1..>760
                     /locus_tag="KJL99_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="porin"
                     /protein_id="HBD4439088.1"
                     /translation="ERILTDRENGEKGDNSQDTSYARVGVKGETQINPEMTGYGQFEL
                     DLEASNRHNPDQTRLAYAGLSYKDFGSFDYSRNVGVAYDAEAFTDMFVEWGGDSWAGT
                     DLFMTNRTNGVATYRNTDFFGMVEGLNFALQYQGKNEGTGNYKANGDGHGLSATYTID
                     GFSFAGAYANSDRTDWQSGDGKGERAEVWALSTKYDANNVYAAVMYGESHNMNSDDGD
                     VVNKTQNFEAVLQYQFDFGLRPSIGYSYSKALDVA"
BASE COUNT          211 a          155 c          184 g          210 t
ORIGIN      
        1 tgagcgcatt ctgaccgacc gtgaaaatgg tgaaaaaggc gataattctc aagatacctc
       61 ttatgcccgt gtaggcgtga aaggtgaaac tcagatcaac cctgaaatga ctggttacgg
      121 tcagtttgaa ctagacctgg aagcatcaaa cagacacaac cctgaccaaa ctcgtttggc
      181 atatgctggt ttgagttaca aagattttgg ttcattcgac tacagtcgta atgtcggtgt
      241 cgcgtatgat gcagaagcat ttactgatat gtttgttgag tggggcggtg actcatgggc
      301 tggtactgac ctgttcatga ccaaccgtac caacggtgtt gcaacctatc gtaacacaga
      361 tttctttggc atggttgaag gtctaaattt tgctctgcaa tatcagggta aaaatgaagg
      421 tacaggcaac tacaaagcaa atggcgatgg ccatggttta tctgcaactt acactattga
      481 cggtttctca tttgctggtg cttatgcaaa ctctgaccgt actgattggc agtctggtga
      541 tggtaaaggc gaaagagccg aagtttgggc gttgtcaaca aaatatgacg ctaacaatgt
      601 ttatgctgct gtgatgtacg gtgaatctca caacatgaat tccgacgacg gtgatgttgt
      661 gaacaaaaca caaaacttcg aagctgttct gcaatatcag ttcgactttg gtctgcgtcc
      721 atccatcggt tactcctatt ctaaagcact ggacgtagca
//
LOCUS       DAEBXN010000381          751 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.391, whole genome shotgun sequence.
ACCESSION   DAEBXN010000381 DAEBXN010000000
VERSION     DAEBXN010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..751
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.391"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            18..751
                     /locus_tag="KJL99_004687"
                     /pseudo
     CDS             18..751
                     /locus_tag="KJL99_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707382.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          240 a          114 c          147 g          250 t
ORIGIN      
        1 ataattttgt taacaatatg aggcgggaga tgttttctac aaacttaata cagagtaatt
       61 atggtgattt aaatattaaa agcctggctt tcgactcttt taaagaaagg cttgagtcta
      121 ccatggcggc actcactttc tttatatcaa cgggtcaatg tgactgcgat gaaatagcag
      181 agtcgaactt caatcgtatg attgcctata tgtctaacat taactatgat gcctcaaagc
      241 taggagcgcc agcacttagt tttgatacgt atctgcagga caatgttaaa tatagagtga
      301 taattaataa tctttatggc agtgagtttc ggataagaga tattaatgag aattttgttg
      361 ggatggattt tacctgtgaa ttccgacccg aaaaaatgac gaatttgatc aatataaaaa
      421 ataggttggt tattcattat ttaaggacta tatactatga aaaaaattat atacatcctg
      481 ccgggagcat ttttgctgcg attcaaaaaa aatgagagtc tcctaaagtt tccttcgata
      541 atatcaatgc ttaatatcaa tcttcttttt aatccactaa atcttccggg aatgggaagt
      601 ggtgtgcttg aagatattat gagtattcca gatagttcac ttagaaaacg tctcgggtat
      661 gaggttttaa gcttctcact acaggcacat tcattgagcc aggaatgtat tgataagttg
      721 gacatttttt tcgctgacga tctgtttaaa t
//
LOCUS       DAEBXN010000382          750 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.392, whole genome shotgun sequence.
ACCESSION   DAEBXN010000382 DAEBXN010000000
VERSION     DAEBXN010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..750
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.392"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            32..493
                     /locus_tag="KJL99_004688"
     CDS             32..493
                     /locus_tag="KJL99_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HBD4439089.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKSTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(534..698)
                     /locus_tag="KJL99_004689"
                     /pseudo
     CDS             complement(534..698)
                     /locus_tag="KJL99_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
BASE COUNT          218 a          162 c          171 g          199 t
ORIGIN      
        1 agtttcttga ttaaataaaa tggagtttta catgaaggct ttcaataagc tgttttccct
       61 cgttgttgca tctgttctgg ttttctctct tgctggctgc ggtgacaaag aagaatcgaa
      121 gaaattcagc gccaatctga acggcactga aattgccatt acctatgtct acaaaggtga
      181 caaggtgctt aagcaatctt ctgaaaccaa aattcaattt gcctccattg gtgcaaccac
      241 caaagaagat gctgccaaga cacttgagcc gttaagcgcc aaatacaaaa acatcgcggg
      301 tgttgaagaa aaatcaacct ataccgatac ctacgcgcag gaaaacgtga ctatcgatat
      361 ggaaaaagtg gattttaaag ccctgcaggg tatttcagga atcaacgttt ctgctgaaga
      421 tgccaaaaaa ggtatcacta tggcgcaaat ggaactggtg atgaaagccg ctggttttaa
      481 agaagtgaaa taatctgtcg gcggccatgt ttcgcatggc cgccataccc gtcttagctt
      541 ttcttcacat gctggcgcgc tgccagtcca cgcagaaaat aacgtaaaaa ttgatcgccg
      601 cattcgcgga agtttttatg atccggtgcg cgcatcatcg ctgtaatttc cggcatcgaa
      661 acgcggaact gctgttcggt gaggatagcc agaatgtcgg taatgactcc aacttactga
      721 tagtgtttta tgttcagata atgcccgatg
//
LOCUS       DAEBXN010000383          704 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.393, whole genome shotgun sequence.
ACCESSION   DAEBXN010000383 DAEBXN010000000
VERSION     DAEBXN010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.393"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(<1..443)
                     /locus_tag="KJL99_004690"
     CDS             complement(<1..443)
                     /locus_tag="KJL99_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBD4439090.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALAFQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPD"
BASE COUNT          214 a          172 c          131 g          187 t
ORIGIN      
        1 tcatctggat aatcggcatg agcaataaca tgcttgccgg tgcgactgtg tacttcaaga
       61 ataaagggca aatgctgaca tttttccagc gccgttttcg cttgtttctg ttgattatct
      121 gccagcgcaa taaaccagtc gccgccattc atcaaccaca aagacatctg ctggaatgcc
      181 agcgcatcca tcgccatctg ttcatgattg cctcttaccg cacgaaccca atgttgttcc
      241 agtaactgca gacaacgtaa actttgcggc ccacgatcga taacgtctcc cactgagata
      301 agtaaatctc gccacggatc aaaacgacaa tgccataatt tgcggcgcaa ctgctcaaga
      361 caaccgtgta tatcgccaga aagccagata tgtcgccatt gatgacccgc aattctctga
      421 taaacgggcg caggctgttt catcaatatt ttcctcccgc gctaaagatc acataatctt
      481 aacaagaatg ttaaaaaacg ctggactcag acagtagagt gtgtgttatg gttgactata
      541 aagtcagcga aggaaatgct tctggctttt aacagataaa aagagaccga acatgattcc
      601 tgttttcgtc aacaaacaat aaaacccttt aaaattaatg cgttgaataa atttacgttc
      661 atcttttaat ccctgacacg taccattgca tattaataca ttca
//
LOCUS       DAEBXN010000384          699 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.394, whole genome shotgun sequence.
ACCESSION   DAEBXN010000384 DAEBXN010000000
VERSION     DAEBXN010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..699
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.394"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(<1..305)
                     /locus_tag="KJL99_004691"
     CDS             complement(<1..305)
                     /locus_tag="KJL99_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4439091.1"
                     /translation="MALFQRAINTQAYLKAGIMGFAGDGKTYTASELAIGLVMLMRQR
                     GIEAGNRPVMFLDTETGSDWVKPRFDAENIELFTAKTRSFVDLLEAINEAESSGSVM"
     gene            complement(311..571)
                     /locus_tag="KJL99_004692"
     CDS             complement(311..571)
                     /locus_tag="KJL99_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor protein Gam"
                     /protein_id="HBD4439092.1"
                     /translation="MAWARHYQQIVREEKETELADDLEKGLPQHLFESLCIDHLQRHG
                     ANKQAISHAFDDDVEFQERMAEHIRYMAETIAHHQVDIDLED"
BASE COUNT          177 a          173 c          155 g          194 t
ORIGIN      
        1 atcattacgg aaccgctgct ttccgcttca ttaatggctt caagcagatc aacaaacgag
       61 cgggtttttg ccgtaaataa ttcgatattt tccgcatcaa agcggggttt aacccagtca
      121 gagcctgttt ctgtgtccag aaacattacc gggcggttac cagcttcaat tccccgttgc
      181 cgcataagca ttaccagacc aatagccagt tcgcttgctg tgtaggtttt gccgtcgcca
      241 gcaaacccca taattcctgc ttttagatag gcctgtgtgt ttattgctcg ttgaaaaagc
      301 gccatagatt ttagtcctcc aaatcaatat caacctggtg gtgggcaatg gtttcagcca
      361 tgtaccggat gtgttctgcc atgcgctcct gaaactcaac atcatcatca aatgcgtggc
      421 tgattgcctg tttattggca ccgtggcgtt gcaaatggtc gatgcagagc gattcaaaca
      481 ggtgctgagg cagacctttt tccaggtcgt ccgccagttc cgtttctttt tcttcacgaa
      541 cgatctgctg gtagtgacga gcccatgcca tttcttcaat acgatcaaaa atcggataag
      601 cgctctgagg tagcctgagt ttaacggaca ctccttcctg aaatagaatg gcatcagaag
      661 gagctaataa tgagcagaaa aacccaacgt tactctaaa
//
LOCUS       DAEBXN010000385          695 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.395, whole genome shotgun sequence.
ACCESSION   DAEBXN010000385 DAEBXN010000000
VERSION     DAEBXN010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.395"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            1..527
                     /locus_tag="KJL99_004693"
                     /pseudo
     CDS             1..527
                     /locus_tag="KJL99_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            591..>695
                     /locus_tag="KJL99_004694"
     CDS             591..>695
                     /locus_tag="KJL99_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBD4439093.1"
                     /translation="MSGKRYPEEFKTEAVKQVVDRGYSVASVATRLDIT"
BASE COUNT          194 a          143 c          166 g          192 t
ORIGIN      
        1 attgtgctga tcgtgcggat gattactacc atgaccgtct ggcgctgact gttgataatt
       61 caacgatcga cgacaactac gaacactaca catataacgg cacttacaat aatgccgctg
      121 atactcatgt tgtagacgtt tacaacattg gtacggctat tactctggat caggaagttg
      181 atctgtccat cactaataac tctcacgttg cgggtattac gttgactcag ggttatgagt
      241 gggaagatat tgacgacaac actgtcagca ctggcgtaaa cagcagcgaa gtgtttaata
      301 acaccatcac tgttaaagat tctaccgtaa cctctggttc atggactgat gaaggtacta
      361 ctggttggtt cggtaataca ggtaatgcca gcgattatag tggtaaatct aactttgtca
      421 ccgtagatac tgacggagat ggtgttgctg atagcactat tgcaagctgg gatgatgttg
      481 cattagctgt agttgcacat ccgaatgttg ataatgctat gcagacctga atcgccacgg
      541 gtttaacaga cacctcagag tcatttaaga tggcttaaag agaggtgccc atgagcggta
      601 agcgttatcc cgaagagttt aaaactgaag cagtcaaaca ggttgttgat cgcggttatt
      661 ctgttgccag cgttgcaaca cgtctcgata tcacc
//
LOCUS       DAEBXN010000386          692 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.396, whole genome shotgun sequence.
ACCESSION   DAEBXN010000386 DAEBXN010000000
VERSION     DAEBXN010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.396"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(<1..>692)
                     /locus_tag="KJL99_004695"
     CDS             complement(<1..>692)
                     /locus_tag="KJL99_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HBD4439094.1"
                     /translation="NQFVGTIWFINCLFVIIAIDAIFREIVKNNIVILIISLLSFMLS
                     QTVLNHNPLLDPQWFWNIDSAMAYWWLLPLGRCMFLELTRDRFFGKSKIGFIVFSITA
                     IMSAYQLLNQKPLLFKIISIFNADIISSSYIQAINTIITTVGLIIFNIFIAKIICGND
                     FIVRAGRNTLNICGMEYITKLFIPMALAIIGFSVTIPNPICAIIYTCICVYVSDKIGH
                     WLSRTVGGPFLI"
BASE COUNT          242 a          114 c          114 g          222 t
ORIGIN      
        1 tttatcaaaa atggcccacc gactgtcctc gatagccaat ggccaatttt atctgataca
       61 tatacacaaa tacaggtata tatgattgcg cagataggat ttggaatagt cacgctgaag
      121 cctataattg ctaaagccat agggataaat aatttggtga tatactccat tccacatatg
      181 ttaagagtat ttcttccagc tcttacaata aagtcatttc cacaaattat ttttgctatg
      241 aaaatattaa agattatcaa gcctaccgtg gtgataatag tattaatagc ttgaatataa
      301 ctacttgata ttatgtctgc attgaatatt gagataatct taaaaagtaa aggtttctgg
      361 ttaagtagtt gatacgcact cattatagcg gtaattgaga agacaatgaa accaatttta
      421 gattttccaa aaaacctgtc acgtgttaat tctaaaaaca tacatctacc gagaggcaat
      481 aaccaccagt atgccatagc ggagtctatg ttccaaaacc attgaggatc tagtaatggg
      541 ttatgattga gcactgtttg tgacaacatg aacgataaca gtgatatgat taaaatcact
      601 atattgttct tgactatttc tctaaagatt gcatcaattg ctattataac aaaaaggcag
      661 tttataaacc atattgttcc tacgaattgg tt
//
LOCUS       DAEBXN010000387          683 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.397, whole genome shotgun sequence.
ACCESSION   DAEBXN010000387 DAEBXN010000000
VERSION     DAEBXN010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.397"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(29..397)
                     /locus_tag="KJL99_004696"
                     /pseudo
     CDS             complement(29..397)
                     /locus_tag="KJL99_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT          211 a          120 c          145 g          207 t
ORIGIN      
        1 ttgtctggag attcaggggg ccagtctaat cagttgtcat gcactcggaa taaatcatac
       61 cgtgaatagt gctgtttttg attgtcagac tattggcgtt ggtattggta gaaataccgt
      121 cgtccagata gtaggtagaa atcacgccgt taacggtagc gttattaatg accgggtaga
      181 tatcgccgtt gtaatagcta tcagcggcat agttgttcca gcctacataa ccatcatagt
      241 aaacgtcatc agcataggtg gcgtcgttgt agtgatggaa agtattgtaa gtttgaccag
      301 aaatatcgtt ggtcgctgca ttagcctgag aggtgatagc cagagtgcaa gccaatgcta
      361 attgtgatac tacaagtttc tttttccagg agtgcatttg tcatccctcc tcagggacgt
      421 aataagttga tccatcaatt atcaatgcat agaaaacata ttatgtttcg cgctaacgat
      481 tatgctgcgt tacagacgaa tggttcaatt attctttccc ctaagtccga atgtggacat
      541 atataattta tggagaaaaa cacgcatcaa attgataaaa ggacgatatt tcagtatgtt
      601 gcagataatg ttatttttga tgcatacgcg ttataaatat caaaaataca aaccagataa
      661 atatcttatc aatatacaac aaa
//
LOCUS       DAEBXN010000388          670 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.398, whole genome shotgun sequence.
ACCESSION   DAEBXN010000388 DAEBXN010000000
VERSION     DAEBXN010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.398"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..141
                     /locus_tag="KJL99_004697"
     CDS             <1..141
                     /locus_tag="KJL99_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBD4439095.1"
                     /translation="RNVVERCFGRLKEYRRIATRYDKTARNYLAMVKLGCIRLFYQRL
                     RN"
     gene            256..>670
                     /locus_tag="KJL99_004698"
     CDS             256..>670
                     /locus_tag="KJL99_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630-like element IS630 family transposase"
                     /protein_id="HBD4439096.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLNAGTVRRWLPS"
BASE COUNT          170 a          179 c          174 g          147 t
ORIGIN      
        1 cgtaatgtcg ttgaacggtg ctttgggcgg ctgaaagaat atcgccgcat cgccacgcgt
       61 tacgacaaaa cggcgagaaa ttacctggcg atggtgaaac tgggctgcat ccgactcttt
      121 tatcaacgct tacgtaatta agggacacag cctaaatagc tgcgcggaat agtagatccc
      181 tttgagggaa cttagcccgg attgtgcgat ctgatcaatc gccaaatcaa aacaaatcac
      241 caaccggact gagcaatgcc gatcatagca ccaatttccc gtgacgaacg acgcctgatg
      301 cagaaagcca tccataaaac acacgataaa aattatgccc gcagactgac tgccatgctg
      361 atgctgcacc ggggcgaccg tgtcagcgac gttgccagaa cgctctgctg cgcccgttcc
      421 tctgttggac gctggattaa ctggttcacg cagtcgggtg ttgagggact gaaatcatta
      481 cctgccgggc gtgcccgtcg ctggccgttt gagcatatct gcacactgtt acgtgagctg
      541 gtaaaacatt ctcccggcga ctttggctac cagcgttcac gctggagtac agaactgctg
      601 gcaataaaaa tcaatgagat aaccggctgc cagttaaatg ccggaaccgt tcgccgctgg
      661 ttgccgtctg
//
LOCUS       DAEBXN010000389          668 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.399, whole genome shotgun sequence.
ACCESSION   DAEBXN010000389 DAEBXN010000000
VERSION     DAEBXN010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.399"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..>668
                     /locus_tag="KJL99_004699"
     CDS             <1..>668
                     /locus_tag="KJL99_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110-like element ISSfl4 family transposase"
                     /protein_id="HBD4439097.1"
                     /translation="ARTLPHALRTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIRG
                     LLTQIHPAPERVLGPRLEHPAVLDLLQRYPSPEKLASLGEKKLAAQLCKLAPRLGKRL
                     AADIAQALAEQTVVVPGTNAAAVVLPRLALQLITLRKQRDEVALEVEQRVLAHPLYPV
                     LTSMPGVGVRTAARLLTEVACRAFASAAHLAAYAGLAPVTRRSGSSIRGEHPSRRGNK
                     ALKR"
BASE COUNT          133 a          230 c          177 g          128 t
ORIGIN      
        1 ctgcccgtac cctgcctcac gcgctacgca cgctgaaact ggctgacgag caaatcgccg
       61 aactctccat gctctgcggc ttcgatgatg atcttgccgc acagacaacg caggccagca
      121 accgtatccg cggccttctg acccagatac atccggcacc ggagcgcgtt ctcggtccga
      181 gacttgagca cccggcggta ctcgatcttc tccagcgata tccctcacca gaaaaactcg
      241 cttcgctggg tgagaagaag ctggcagccc agctctgcaa acttgcgcct cgtctgggta
      301 aacgccttgc agcagacata gctcaggcac tggccgaaca aaccgtcgtc gttcccggca
      361 cgaatgccgc tgccgtagta ctgccacgtc tggcactcca gctcatcacg ctgcgtaagc
      421 aaagagacga ggtggcgctt gaggtagaac agcgagttct tgctcaccct ctttacccgg
      481 tcctgaccag tatgcccgga gtcggtgtca ggaccgcagc cagactcctc accgaggtcg
      541 cctgccgcgc cttcgcctct gccgcacatc tcgctgctta tgctggcctt gcgccggtaa
      601 ctcggcgatc cggctcgtca atacgcggtg agcatccctc gcgacggggt aataaagctc
      661 tcaaacgg
//
LOCUS       DAEBXN010000390          660 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.400, whole genome shotgun sequence.
ACCESSION   DAEBXN010000390 DAEBXN010000000
VERSION     DAEBXN010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.400"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(<1..>660)
                     /gene="rfbG"
                     /locus_tag="KJL99_004700"
     CDS             complement(<1..>660)
                     /gene="rfbG"
                     /locus_tag="KJL99_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dTDP-rhamnosyl transferase RfbG"
                     /protein_id="HBD4439098.1"
                     /translation="RLIKDVDLENYDYICFSDQDDIWYKDKIKNAIDCLVFNNANCYS
                     SNVIAYYPSGRKNLVDKAQSQTQFDYFFEAAGPGCTYVIKKETLIEFKKFIINNKNAA
                     QDICLHDWFLYSFARTRNYSWYIDRKPTMLYRQHENNQVGANISFKAKYKRLGLVRNK
                     WYRKEVTKIANALADDSFVNNQLGKGYIGNLILALSFWKLRRKKADKIYILLMLILNI
                     F"
BASE COUNT          214 a          129 c           76 g          241 t
ORIGIN      
        1 taaaatatgt ttaaaatcaa cattaataaa atataaattt tatcagcctt tttccgcctt
       61 aacttccaaa aacttaaagc caagatcaaa ttgcctatat aacctttacc caattgatta
      121 ttcacaaagc tatcatcagc taaggcatta gctattttag ttacttcttt tcgataccac
      181 ttgtttctca ccaaaccaag ccgtttgtat tttgctttaa aactaatgtt tgctcccact
      241 tggttgtttt catgctgtct gtacagcatt gttggttttc tatcaatata ccacgaataa
      301 tttctcgtcc gcgcaaatga atataaaaac caatcatgca gacagatatc ttgtgcagca
      361 tttttattat ttatgatgaa cttcttaaat tcaataagcg tttctttttt tataacatat
      421 gtacaccccg gacctgctgc ttcaaaaaaa taatcgaatt gagtttgcga ctgagcctta
      481 tcaactaaat tttttcgccc tgatggataa tatgcaatta cattgctgga ataacaattc
      541 gcattattaa ataccaagca gtcaattgca ttttttattt tatctttata ccaaatatcg
      601 tcctgatcac taaaacagat ataatcatag ttttcaagat cgacatcttt aattaatcta
//
LOCUS       DAEBXN010000391          660 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.401, whole genome shotgun sequence.
ACCESSION   DAEBXN010000391 DAEBXN010000000
VERSION     DAEBXN010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.401"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..>660
                     /locus_tag="KJL99_004701"
     CDS             <1..>660
                     /locus_tag="KJL99_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HBD4439099.1"
                     /translation="LDAGGLRDIEVEAVTKMLACGTRILGVKEYICDKPECPHVRYVT
                     NSCGSRACPSCGKKATDLWIATQLNRLPDCDWVHLVFTLPDTQWPVFESNRWLLNDVC
                     RLAVENLLYAARKRGQEPGIFCAIHTYGRRLNWHPHVHVSVTCGGLNKHGQWKKLSFL
                     KDAIRSRWMWNMRQRLLKAWSEGLAMPESLSHITTESQRRSLVLKAGGKYWHVYMSKK
                     T"
BASE COUNT          153 a          167 c          208 g          132 t
ORIGIN      
        1 tcctggatgc gggcggtctg cgcgatatcg aggttgaggc tgtcacaaaa atgctggcct
       61 gcggcacgcg gatactgggc gtgaaggaat acatctgcga taaacctgag tgcccccacg
      121 tcagatacgt cactaactca tgcggcagcc gtgcctgccc gtcctgcgga aaaaaggcca
      181 cagacctgtg gatagcgaca cagctgaatc gtcttcctga ctgcgactgg gtacacctgg
      241 tcttcaccct gccggacacg cagtggccgg tgttcgaaag caaccggtgg ctgctgaatg
      301 acgtgtgccg tctggcggtg gagaatctgc tgtatgccgc ccggaaacgg gggcaggaac
      361 ccggtatctt ctgcgccatc cacacgtatg gccgtcgtct caactggcat ccgcatgtac
      421 atgtgtctgt aacctgtgga ggtctgaata agcatggtca gtggaaaaag ctgagcttcc
      481 tgaaagacgc gatacgttcg cggtggatgt ggaatatgcg gcaacggctt ctgaaagcgt
      541 ggtcagaggg gctggcgatg ccggagtcgt tgtcacatat cacgacggaa tcacagagga
      601 gaagcctggt gctgaaagcc ggaggaaaat actggcatgt gtacatgtcg aaaaaaacgg
//
LOCUS       DAEBXN010000392          656 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.402, whole genome shotgun sequence.
ACCESSION   DAEBXN010000392 DAEBXN010000000
VERSION     DAEBXN010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.402"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            52..>656
                     /locus_tag="KJL99_004702"
     CDS             52..>656
                     /locus_tag="KJL99_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HBD4439100.1"
                     /translation="MPVFHNTRVLVEPEPKSMRNLPSGVVPAVRQPLVEDKTLLPFFS
                     NARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHHSETVIHRYGTGAMVLCWHCDNQLR
                     DQTSESLEQLAHQNLSAWMIDVIGHAISGTQERELSLAELSWWAVRNQVADALPEAVL
                     RRSLGLRAEKIRSMYRESDIVPGEQTATSILKQRTKNLAPLP"
BASE COUNT          144 a          179 c          189 g          144 t
ORIGIN      
        1 gttctggttc cggaactcgg gctggtggtc cttaagcccg gtcgtgaatc catgccggta
       61 ttccacaata cccgggtact ggtggagccg gaaccgaaaa gcatgcgtaa tctgccgtcc
      121 ggggtcgttc ctgccgttcg ccagccgcta gtggaagaca aaacattgct gccgtttttc
      181 agtaacgcac gggtgattcg tgctgctggt ggtgctggcg cattgtctga ctggctgttg
      241 cgccatatta aatcctgcca gtggccacac ggcgattatc atcacagcga aaccgtcatt
      301 caccgttatg gtaccggcgc aatggtattg tgctggcact gcgacaacca gctgcgtgac
      361 cagacatccg aatcactcga gcaacttgct catcaaaacc tgtcagcatg gatgattgac
      421 gtcatcggtc acgcaataag cggtacgcag gagcgtgaat tatctctggc tgaattatcc
      481 tggtgggcgg tccgcaatca ggtggcggac gcgctaccgg aagcggtatt acgtcgttcg
      541 ctggggttgc gtgcggaaaa aatccgctca atgtaccgtg aaagcgacat cgtgccggga
      601 gagcagaccg ccaccagcat actgaagcag cgcacaaaaa atcttgcgcc gctgcc
//
LOCUS       DAEBXN010000393          656 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.403, whole genome shotgun sequence.
ACCESSION   DAEBXN010000393 DAEBXN010000000
VERSION     DAEBXN010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.403"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(151..648)
                     /locus_tag="KJL99_004703"
     CDS             complement(151..648)
                     /locus_tag="KJL99_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005075795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HBD4439101.1"
                     /translation="MTTELLAQLYGTEPVRIRQNHHENKVRFVEGKHFFKVVGNDLKE
                     LRVALNYSQNPVSPKARSLILWTERGAARHGKMLETDQAWDVFEKLEDCYFSQRDPST
                     PVSCQKSYDTRVLCYQRGGVTVSTIQLRDDDIVISLESWLELARANGWFVVRRDKLVE
                     RLMQL"
BASE COUNT          173 a          160 c          144 g          179 t
ORIGIN      
        1 gtttaacgcc ctgtgccatt tttcaggcac aaaaaaaccc gcataaagcg ggttctttca
       61 agtgtccatg tctgctattc gcctcgcgat acagctttgc gaagcttacc gtaattgaag
      121 cagtttatgt gcaaaaatgc aagaactttt ttaaagctgc atcaaccttt ccaccagttt
      181 atctctgcga acaacaaacc aaccattggc tctcgccagt tccagccatg actcaaggga
      241 aataacaata tcatcatccc gtaactgaat tgtggaaaca gtgacaccgc ctctctgata
      301 acagagaact cgcgtgtcgt aacttttctg gcatgaaact ggcgttgacg gatccctttg
      361 actgaaatag cagtcttcca gtttttcgaa cacatcccac gcctgatcgg tttcgagcat
      421 tttgccgtgg cgtgctgcgc ctcgttctgt ccagaggatg agggagcggg ctttgggtga
      481 aactggattt tgtgagtagt ttaaagcgac ccgcaattct ttaaggtcat taccaacaac
      541 tttgaaaaag tgtttccctt caacgaagcg tactttgttc tcatgatgat tctggcgaat
      601 acgcaccggc tcagtgccgt aaagctgcgc caaaagttcg gtggtaataa caggaa
//
LOCUS       DAEBXN010000394          594 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.404, whole genome shotgun sequence.
ACCESSION   DAEBXN010000394 DAEBXN010000000
VERSION     DAEBXN010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.404"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            10..>594
                     /locus_tag="KJL99_004704"
     CDS             10..>594
                     /locus_tag="KJL99_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HBD4439102.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPYCPWQHIVF
                     TLPCQYWSLVFHNRWLLAEMSRIAADVILEICHQADVEPGIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITR"
BASE COUNT          130 a          157 c          172 g          135 t
ORIGIN      
        1 gcccttctga tgctcccccg ttttgccgat atttttcagc agggaaaccg ctggcttaac
       61 tggctggaga aacaaccgga aggggcagtc cgtccggtgg taattgaatc agtgacaaaa
      121 atcatggcct gcgggaccac actgatgggc tacacgcagt ggtgttgttc atcaccggac
      181 tgttgccata caaaaaaggt ctgcttccgg tgtaaaagcc gctcctgccc gcactgcggg
      241 gtgaaggctg gcgcacagtg gatacagtat ctgctgagcc tggtccccta ctgcccgtgg
      301 cagcatattg tgttcacact tccctgccag tactggtccc tggtgttcca caaccggtgg
      361 ttactggcag agatgagccg cattgcagcg gatgtgatac tggaaatctg ccatcaggca
      421 gatgtggagc cggggatatt cacggtgatc cacacatggg ggcgtgacca gcagtggcat
      481 ccgcatatcc atttatcgac aactgccggt ggtgtgacgt cgggccacac ctggaaaaat
      541 cttcattttt acgcccgtaa ggtgatgagc atgtggcgct accggataac acgg
//
LOCUS       DAEBXN010000395          588 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.405, whole genome shotgun sequence.
ACCESSION   DAEBXN010000395 DAEBXN010000000
VERSION     DAEBXN010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.405"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..109
                     /locus_tag="KJL99_004705"
     CDS             <1..109
                     /locus_tag="KJL99_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HBD4439103.1"
                     /translation="RYDRRLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
     gene            167..523
                     /locus_tag="KJL99_004706"
     CDS             167..523
                     /locus_tag="KJL99_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4439104.1"
                     /translation="MEIKPEDELSNIVLFPIKEDDPRNQVNFLYKPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
BASE COUNT          186 a          115 c          151 g          136 t
ORIGIN      
        1 ccgctacgac aggcgcctga atataatctg tcgcgagtgc cggaagagcg aagctatgca
       61 gcgtgtactg gctttctatc agggtaattt tcaggaggcg atactgtgaa tgaaattagc
      121 tatcaggctt caattaccgc tggcattcgc atcaaaggag aggagcatgg aaataaaacc
      181 agaagatgag ttaagcaata tcgttttatt tccgataaaa gaggatgacc ctcgtaatca
      241 ggttaatttt ctttataagc catcggaaag accatattgc catcacgcct ctgtacgggt
      301 tgacgaaaaa gagcgtcagg tccgctgtaa aatctgcggt gcggttgtgg agccgtttga
      361 ctggatgctc tctgtggcaa aaagagaaac cagactggca gatgatgtaa ggctattgcg
      421 ccaggaggaa caggaaaggc ggagaaatat agaaaagctg atacagattg agcgtaacgc
      481 gaaagcgcgg atacgcaggg cgacaaaatc cagaactgaa taattaaatt tagcactgtt
      541 aaaaatttaa tccttaaccg gagggatttc tgcaccctca aatcatca
//
LOCUS       DAEBXN010000396          580 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.406, whole genome shotgun sequence.
ACCESSION   DAEBXN010000396 DAEBXN010000000
VERSION     DAEBXN010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.406"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            31..318
                     /locus_tag="KJL99_004707"
     CDS             31..318
                     /locus_tag="KJL99_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005046668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HBD4439105.1"
                     /translation="MFTVNGQVIPLNYGNFDARYKYRTEGVQDVRYGHEMYYSPGSNT
                     VSWRFCAPSGHGLSGMAISDTGRNSADNVDGVYYRPLQKLINGTWYNVASI"
     gene            321..>580
                     /locus_tag="KJL99_004708"
     CDS             321..>580
                     /locus_tag="KJL99_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HBD4439106.1"
                     /translation="MLHLKNITAGNPKTAEQYQMTKRYSVTWLFSEDGKNWYEELKNF
                     ASDTIKIAYTGDGRVVWVGKDVTGIEPRNASVIEVPDITANRR"
BASE COUNT          178 a          122 c          149 g          131 t
ORIGIN      
        1 ttcagccaga gaaacccgga taacagcata gtgttcactg ttaacggaca ggtaattccg
       61 ttaaattacg gaaacttcga tgcccgctat aaatatcgaa cagagggggt acaggatgta
      121 cggtatggcc atgaaatgta ttacagcccc ggcagtaaca ccgtttcgtg gagattttgc
      181 gcaccttcgg gacacgggct gtcagggatg gcgatatcgg ataccggccg taactcagcg
      241 gataacgttg acggtgtgta ttaccgacca ctgcaaaaac tgattaatgg cacctggtat
      301 aacgtagcga gtatttaaca atgttgcatt taaaaaatat tactgcgggt aatccgaaaa
      361 cagcagaaca atatcagatg acaaaacgat attcggtcac ctggcttttt tcagaagacg
      421 gaaaaaactg gtatgaagag ctgaagaatt tcgccagcga cacaataaaa atagcttaca
      481 ccggagatgg tcgcgtggtg tgggtcggta aggatgtgac aggcatcgag ccacgcaatg
      541 ccagcgttat cgaagttccg gatattaccg ctaaccgacg
//
LOCUS       DAEBXN010000397          566 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.407, whole genome shotgun sequence.
ACCESSION   DAEBXN010000397 DAEBXN010000000
VERSION     DAEBXN010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.407"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..297
                     /locus_tag="KJL99_004709"
     CDS             <1..297
                     /locus_tag="KJL99_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HBD4439107.1"
                     /translation="SSAQYSGEIKGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPG
                     IDDSVDLSRYSPELDSHGQYSLPASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(405..>566)
                     /locus_tag="KJL99_004710"
     CDS             complement(405..>566)
                     /locus_tag="KJL99_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4439108.1"
                     /translation="LQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVDGKRSGKINQTT
                     TKMLFMCRE"
BASE COUNT          185 a          133 c          104 g          144 t
ORIGIN      
        1 agcagcgctc agtactccgg cgaaataaag ggatacgatt acgatacata taccttctat
       61 gccaaaaaag gtcagaaagt gcatgtgagt atctctaatg aaggcgctga tacttatctc
      121 ttcgggccag gaatcgatga ttctgttgat ttatccagat attccccaga actcgatagt
      181 cacggacaat actcactccc tgcttctggc aaatatgagc tgcgagtgct gcaaactcga
      241 aacgacgccc gtaaaaacaa aactaaaaaa tataacgtcg atattcaaat taagtaaatg
      301 tcaaccgacg gggactatcc ccgtcataga cgttaacgcc ccactcctct ctgtcgctca
      361 agaggttaat gacgaatagc agagaacata atgtaaacaa ggcatcactc acgacacata
      421 aataacatct ttgtggtggt ttgattgatt tttccactcc gcttaccatc tacaccaaaa
      481 taacgatcat cacgacataa tttttcttta acatcgcaaa atacaccgtt agcgaatgta
      541 aattcgctgg gatcgtagcc ttgtaa
//
LOCUS       DAEBXN010000398          551 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.408, whole genome shotgun sequence.
ACCESSION   DAEBXN010000398 DAEBXN010000000
VERSION     DAEBXN010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..551
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.408"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(<1..>551)
                     /locus_tag="KJL99_004711"
     CDS             complement(<1..>551)
                     /locus_tag="KJL99_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MolR family transcriptional regulator"
                     /protein_id="HBD4439109.1"
                     /translation="VEQAKDNSLQPSQTVTDSLKVADLSTIIQEQPSFVAETRAADKN
                     TDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVSQRDNKT
                     ATFDFSTCSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVREDVMDQ
                     IVQEGSLEYATDVIIHLQQIDIK"
BASE COUNT          134 a          117 c          135 g          165 t
ORIGIN      
        1 atttaatatc aatctgttga aggtgtatta ttacatcagt agcatattcc agactgcctt
       61 cctggacgat ctgatccata acatcctcac gcactttata tcttgtgcat ttcatttcaa
      121 gtgccgtgag tacagccatt actggtgatg gtagctgtgt tgttttcagg ccgtcgatct
      181 gactgatcgc ttgggcgacg gtgttttgcc attctaaaga acaggtgctg aagtcaaatg
      241 tggctgtttt attatcccgt tgcgacacgc tacaggctaa gcgacgtaat tgggtcaatt
      301 tgtctgcttg ctgaacagga actccgggaa agcggcgatg acttaacgtg gtgtgtacaa
      361 cttcgggcgg aaaaacgaca gcaatatctt tcgccagcca aggtaaaaca gcatctgtat
      421 ttttgtcagc cgcacgggtt tctgctacga aagagggttg ttcctgaata atggtggata
      481 agtccgctac cttgagagag tccgttaccg tttgggaagg ttgcaaagaa ttatctttcg
      541 cttgctccac a
//
LOCUS       DAEBXN010000399          535 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.409, whole genome shotgun sequence.
ACCESSION   DAEBXN010000399 DAEBXN010000000
VERSION     DAEBXN010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..535
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.409"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(427..>535)
                     /locus_tag="KJL99_004712"
     CDS             complement(427..>535)
                     /locus_tag="KJL99_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HBD4439110.1"
                     /translation="NSGDTMLDNAMKSKDSKMIDFLLKNGAILGKRFEI"
BASE COUNT          138 a          124 c          110 g          163 t
ORIGIN      
        1 ccttcactga tcgaaagctg attttcagga accgttttga cggcttcggc tttaaactcc
       61 gtagagtaac gttgggtttt tctgctcatt attggctcct tctgatgcca ttctatttca
      121 ggaaggagtg tccgttaaac tcaggctacc tcactcccgt aaagacagga gtcaagttcc
      181 cgctccgaca taactgcgtg caatggattt tgaaggaacc gtcagcccat tccccggagg
      241 caaacatggg ctggcaaaag tgtagcggta ttaggcacag ctatttagga aagagggacc
      301 atctccatct cattaaatgg accacgccag acaattcact gtcatgctga tgctgcactg
      361 tggggacaga atcactcatg atgacttcga taatcgacga cattattatt tggccgagct
      421 tttaggtcat atctcaaatc gtttgcctaa tattgctcca ttttttaata aaaaatcaat
      481 catttttgaa tctttgcttt tcattgcatt gtcaagcata gtgtccccac tattt
//
LOCUS       DAEBXN010000400          528 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.410, whole genome shotgun sequence.
ACCESSION   DAEBXN010000400 DAEBXN010000000
VERSION     DAEBXN010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.410"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..228
                     /locus_tag="KJL99_004713"
     CDS             <1..228
                     /locus_tag="KJL99_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259191.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HBD4439111.1"
                     /translation="ESNSDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLVNRNCYLD
                     KYGDARINASYTQLEIKHSQPKNRASGKRVS"
BASE COUNT          182 a          110 c           84 g          152 t
ORIGIN      
        1 gaaagcaatt ctgacagcgc cccctatacg ttattaaaaa actatgctga cagatttaaa
       61 aaatattcag cagaagagca gtacgaaatt gattttcaac atcgactggt aaacagaaat
      121 tgttatttag ataaatatgg cgatgcacga atcaatgctt cttatacaca attagaaata
      181 aaacactcac aacccaaaaa tagagcatcc ggcaaaagag tgagttaaca tcattgttaa
      241 tcaattcgac cagtcagtaa atatgctgac tggtcgaaca taatttgcta aaaagtcatc
      301 actgactgat gtcagtccca ccatctgcca gttcaatcta cgggtatttc cagcatatcg
      361 cggcacgcga ttgctggtct gttgatgtag tggattgtag ttgctgactt ttcttaccct
      421 cagatttctg ccccacttgt caattcaatc tgccaataaa taacataatt atatttttat
      481 tcctcacaga aattcctttt taaaaatagc gaacccactg taaaaaac
//
LOCUS       DAEBXN010000401          527 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.411, whole genome shotgun sequence.
ACCESSION   DAEBXN010000401 DAEBXN010000000
VERSION     DAEBXN010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.411"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(<1..>527)
                     /gene="capU"
                     /locus_tag="KJL99_004714"
     CDS             complement(<1..>527)
                     /gene="capU"
                     /locus_tag="KJL99_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HBD4439112.1"
                     /translation="KKMGHSVLLVCRENSKIAFEASKFGIDITFALFRNSLHIPTVWR
                     LLGIVHSFQPDAIVCHSGHDSNIVGLVRFFTWKHPFRIIRQKTYLTRKTKFFSINHFC
                     DEVIVPGTNMKTHLEQEGCRTRVTVVPPGFDFQELYVDSRNSLPPSVLSWLASRRGCP
                     VIAQVGMLRPEKGHE"
BASE COUNT          158 a          124 c           99 g          146 t
ORIGIN      
        1 ttcgtgccct ttttccgggc gcaacattcc tacctgagca attacggggc atcctcttcg
       61 ggacgccagc caggaaagaa cactcggagg caaactgttt cgggaatcaa cgtataattc
      121 ctggaaatca aagccaggag gcacaacagt aacccgagtt cgacatcctt cctgctccag
      181 atgtgtcttc atatttgttc cgggaacaat cacctcatcg caaaaatgat ttattgaaaa
      241 aaattttgtt tttcgtgtca aatatgtctt ttgcctgata attctgaatg gatgtttcca
      301 ggtaaaaaac cgtaccaaac caacaatatt actatcatgt ccactgtgac aaacgattgc
      361 atcgggctga aaactatgaa ctattccgag taatctccat acagtaggaa tgtgaagact
      421 gtttcgaaat aacgcgaatg tgatatcaat tccaaactta ctggcttcaa aagcaatttt
      481 gctgttctcc ctgcagacaa gcagaactga atgccccatt ttcttca
//
LOCUS       DAEBXN010000402          525 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.412, whole genome shotgun sequence.
ACCESSION   DAEBXN010000402 DAEBXN010000000
VERSION     DAEBXN010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.412"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            155..340
                     /locus_tag="KJL99_004715"
                     /pseudo
     CDS             155..340
                     /locus_tag="KJL99_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          146 a          129 c          130 g          120 t
ORIGIN      
        1 tacggtggtc ggtttacgct taccgcagga caggtggtaa ctgaaaaata gtttgctgag
       61 gcgactggaa acagaagggc aagatcgatt ccgacagcag tgatggtcag ctacctcatc
      121 gataactttg caacagtgcc cttcttgaag catcatgctt catgaagaaa aactggcacg
      181 tcatcaacgt aaacaggcga tgtatacccg aatggcagcc ttcccggcgg tgaaaacgtt
      241 cgaagagtat gacttcacgt tcgccaccgg agcaccgcag aagcagctcc agtcgttacg
      301 ctcactcagc ttcatagagc gcaatgaaaa catagtttta caggggacat ctgacatcac
      361 aaatccccgg gtggggattt gtgtataaga gacagcccat ggaggaaggg gatatatcgc
      421 agctctgacg actcactcct tatttacccg ggaaatactt ctccagcaat gcatacagga
      481 tggcagcggt ttcagcatcc attatgccgt cataattttt ctgcc
//
LOCUS       DAEBXN010000403          520 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.413, whole genome shotgun sequence.
ACCESSION   DAEBXN010000403 DAEBXN010000000
VERSION     DAEBXN010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.413"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            369..>520
                     /locus_tag="KJL99_004716"
     CDS             369..>520
                     /locus_tag="KJL99_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000082749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HBD4439113.1"
                     /translation="MSQQPQQPQQEISIINLDQLVSMTSVEIAELTGKEHRNVLRDIR
                     KMLEEL"
BASE COUNT          129 a          138 c          113 g          140 t
ORIGIN      
        1 ctagggtctc tctataggtc ttctctatgg tctctctata ggtctctcta gggtctcctc
       61 taggcattac ccccagaagc taactctatg gccctctatg gtcctctatg gccctgtacg
      121 tcattcttta ggatgccctg tagtttcgta tagcctgatg cctagagaga ccctagagag
      181 accctagagt ggctcctaga gagaccctcc cagtagctcc gcacagctac atcgtcatca
      241 tcatagtatt caatctcaaa gtcattctgt ttgcatcctg ttgtcctgct ggtctccctt
      301 cgcttgggga ctttgagtag gtcagtgggg gtgcgacttc agggtgcaaa caaaaaggaa
      361 atcaagcgat gtctcaacaa ccacaacaac cacaacaaga aatcagcatc attaacctcg
      421 accagcttgt gtccatgacc tcagttgaaa ttgctgagct gacaggcaag gagcaccgaa
      481 atgtccttcg tgatattcgt aagatgctcg aagaacttaa
//
LOCUS       DAEBXN010000404          515 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.414, whole genome shotgun sequence.
ACCESSION   DAEBXN010000404 DAEBXN010000000
VERSION     DAEBXN010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.414"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..361
                     /locus_tag="KJL99_004717"
     CDS             <1..361
                     /locus_tag="KJL99_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBD4439114.1"
                     /translation="DHKTGETATETLTQRELVARLKQHIPEKFFKMVRYFGFLANRVC
                     GEKLPQVYRALGMDKPEPVAKVCYAQMVKQFLSRDPFECVLCGGRMVYRRAIAGLNVE
                     GLKKNARDISLLRYMPA"
BASE COUNT          137 a          108 c          176 g           94 t
ORIGIN      
        1 ggaccacaaa acgggagaaa cggcgacgga aacgctgaca cagcgtgagc tggtcgcgag
       61 gctgaaacag cacatcccgg agaagttttt taagatggtg aggtacttcg ggttccttgc
      121 caaccgggtg tgtggagaga agctgccgca ggtgtaccgt gctctgggga tggataaacc
      181 ggaaccagtg gcgaaagtgt gctatgcaca aatggtgaaa cagttcctga gtcgtgaccc
      241 gttcgaatgc gtgctgtgtg gcggccggat ggtataccgc cgggccatcg cgggactgaa
      301 tgtggagggg ctgaagaaaa acgcgcggga tatcagtctg ctgaggtata tgccggcctg
      361 acacaggcta agtgtgcccg ggaatcggtg caagtataaa aaacagcctg ttgcggtata
      421 agaatcgacc atgcagagag agaaaacacc ggagtggagg gaaaagcaga aaagctcacg
      481 tggcataagg cgtggacagc ggtatcgtct ggttt
//
LOCUS       DAEBXN010000405          511 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.415, whole genome shotgun sequence.
ACCESSION   DAEBXN010000405 DAEBXN010000000
VERSION     DAEBXN010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.415"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            1..511
                     /locus_tag="KJL99_004718"
                     /pseudo
     CDS             1..511
                     /locus_tag="KJL99_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT          127 a          128 c          141 g          115 t
ORIGIN      
        1 acaccacggg ccggacgccc atgggctgag caccgtaaaa tcatcaatgg catgttctgg
       61 gtgttatgtt ccagtgcacc atggcgcgat ttacccgaac gatatggggc atggaaaact
      121 gtttataacc gctttaaccg atggtcaaag tcaggcgtga ttaatattat tttcaacagg
      181 ttgctttcgc tacttgatgc aaacggcttt attgactggt ctgccaccgc gctggatggc
      241 agtaatatcc gggcgctgaa atgtgccgcc ggtgctcaaa aaaacatccc gatatcgacg
      301 gagataatgg gccgggtcgc tctcgcggcg gttttggcac caaaatccat ctggcaacag
      361 acggaagtgg cctcccgtta aatatcgtgc taagccccgg acaagctcat gaaagccagt
      421 tcgcgcaacg acttctggac gggattggcg ttcagcgtca gaacggcagc atgaaacgcc
      481 gtggacatgc ggtactggct gataaagcgt a
//
LOCUS       DAEBXN010000406          501 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.416, whole genome shotgun sequence.
ACCESSION   DAEBXN010000406 DAEBXN010000000
VERSION     DAEBXN010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.416"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            1..501
                     /locus_tag="KJL99_004719"
                     /pseudo
     CDS             1..501
                     /locus_tag="KJL99_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS1-like element IS1B family transposase"
BASE COUNT          114 a          132 c          151 g          104 t
ORIGIN      
        1 tgcagttcac ttacaccgct tctcaacccg gtacgcacca gaaaatcatt gatatggcca
       61 tgaatggcgt tggatgccgg gcaaccgccc gcattatggg cgttggcctc aacacgattt
      121 tccgccattt aaaaaactca ggccgcagtc ggtaacctcg cgcatacagc cgggcagtga
      181 cgtcatcgtc tgcgcggaaa tggacgaaca gtggggatac gtcggggcta aatcgcgcca
      241 gcgctggctg ttttacgcgt atgacaggct ccggaagacg gttgttgcgc acgtattcgg
      301 tgaacgcact atggcgacgc tggggcgtct tatgagcctg ctgtcaccct ttgacgtggt
      361 gatatggatg acggatggct ggccgctgta tgaatcccgc ctgaagggaa agctgcacgt
      421 aatcagcaag cgatatacgc agcgaattga gcggcataac ctgaatctga ggcagcacct
      481 ggcacggctg ggacggaagt c
//
LOCUS       DAEBXN010000407          493 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.417, whole genome shotgun sequence.
ACCESSION   DAEBXN010000407 DAEBXN010000000
VERSION     DAEBXN010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.417"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..>493
                     /locus_tag="KJL99_004720"
     CDS             <1..>493
                     /locus_tag="KJL99_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBD4439115.1"
                     /translation="RTRETYGTRRLQTELADNGIIVGRDRLARLRKELRLHCKQKRKF
                     RATTNSDHNLPVTPNLLNQNFTPTAPNQVWVADITYVATREGWLYLAGVKDVYTCEIV
                     GYAMGERMMKELTGKALFMALRSQRLPAGLIHHTDRGSQYCAYDYRVIQEQSGLKTSM
                     SRKG"
BASE COUNT          129 a          128 c          137 g           99 t
ORIGIN      
        1 tccgcactcg cgaaacatat ggaacccggc ggctccagac ggagctggca gataacggaa
       61 ttatcgtcgg tcgtgacaga ctggcacgtc ttcgtaagga gctgaggctg cactgtaagc
      121 agaaacgcaa gttcagggcg accacaaact ctgaccacaa tctgccggtt acgccgaatc
      181 tgctgaatca gaactttacg cccacagcac ctaatcaggt ctgggtcgca gatataacgt
      241 atgttgccac ccgggaagga tggctgtatc tcgccggcgt caaagatgtt tatacgtgcg
      301 aaattgtcgg ttacgccatg ggagagcgca tgatgaaaga gttgacgggt aaagccctgt
      361 ttatggcgct caggagccag cgcctacctg ccgggctaat ccaccacacc gatcgaggtt
      421 cacagtactg cgcatacgat tacagggtca tacaggagca gtctggtctg aaaacatcaa
      481 tgtcgcgtaa agg
//
LOCUS       DAEBXN010000408          490 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.418, whole genome shotgun sequence.
ACCESSION   DAEBXN010000408 DAEBXN010000000
VERSION     DAEBXN010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.418"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
BASE COUNT          149 a          116 c           94 g          131 t
ORIGIN      
        1 ccccgacaga cctgtgctat cggaaagaaa aaaggccatc agcaaaactc tgatgacctg
       61 aatcaccttt accaatattg tatgaaaaaa cacgcacgac taattgacaa taattttcat
      121 ttccattaaa aaagatcgcg tagcattctt aattcatgag gaactttacc cgccagcaat
      181 ctgagtagcg aaagctgttc atccccaatg tttttgctgg cgggtccttt tttctacggt
      241 cctcctcccc ggaggggcat ttcattattt ttctaatcgt tactggtgag aaggcggaag
      301 aaaaagccag ttcttcgccc ctaaataaca gcatgattcc agtccatgca aaatctgatt
      361 tccagctcac acataccgag cactgtacaa aaaaccgcca gccaaggcag ttaagagtgt
      421 gttcccgggg tttgcttaga atattttgta agttgtccga agagatattt acaacaccag
      481 aatgatgcat
//
LOCUS       DAEBXN010000409          489 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.419, whole genome shotgun sequence.
ACCESSION   DAEBXN010000409 DAEBXN010000000
VERSION     DAEBXN010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.419"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..264
                     /locus_tag="KJL99_004721"
     CDS             <1..264
                     /locus_tag="KJL99_004721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HBD4439116.1"
                     /translation="FYRNDEFVFDYRLKAEDERDALLAQVSARTGEWEEDLLLGLISD
                     EDKEKLKACRIYAKSLQAMDFSTITDKSSYNAIEWPASPEGSS"
     gene            288..>489
                     /locus_tag="KJL99_004722"
     CDS             288..>489
                     /locus_tag="KJL99_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HBD4439117.1"
                     /translation="MSVVISGALTDGAGIPMSGYHIILKSRVNTPEVVMNTVADVMTG
                     NDGEYCFHARTGKYGVYLKQDWR"
BASE COUNT          135 a          105 c          130 g          119 t
ORIGIN      
        1 ttttaccgca atgatgaatt tgtctttgac tacagactca aagcggaaga tgagcgtgat
       61 gcccttctgg ctcaggtcag tgcccggaca ggggaatggg aagaagacct gctgctgggg
      121 ttaatcagcg acgaagataa agaaaagctg aaagcctgtc gtatttacgc gaaatcgctg
      181 caggcgatgg atttcagcac catcactgat aaatcctcat acaacgccat tgaatggccc
      241 gcctctccgg aaggttcttc ctgatttaat ttatcgcgag aaaaacaatg tctgtagtga
      301 tatcaggtgc gctgactgat ggcgcaggta tccccatgtc cggataccat attattctga
      361 aatcccgggt aaacaccccg gaagtggtga tgaacactgt tgctgatgtg atgacaggaa
      421 acgatggtga atactgtttc catgcgcgga ctggaaaata tggtgtgtat ctgaaacagg
      481 actggcgca
//
LOCUS       DAEBXN010000410          486 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.420, whole genome shotgun sequence.
ACCESSION   DAEBXN010000410 DAEBXN010000000
VERSION     DAEBXN010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.420"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..>486
                     /locus_tag="KJL99_004723"
     CDS             <1..>486
                     /locus_tag="KJL99_004723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD4439118.1"
                     /translation="LGALWVRNGMEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFL
                     KIIALRLCELLTMYIVTAFALNYSTQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLAD
                     RFGRRRVYITGALIGTLSAFPFFMALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFT
                     E"
BASE COUNT          110 a          107 c          123 g          146 t
ORIGIN      
        1 actgggagca ttgtgggtgc gcaatggcat ggaggagtcc gcggaatttg aacaacagca
       61 acataatcaa gcggccgcga aaaaacgcat cccggttatc gaagctctgt tacgacatcc
      121 cggtgctttc ctgaagatta ttgcactacg actgtgcgag ttgctgacga tgtacattgt
      181 tactgccttt gcacttaatt attcaaccca gaatatgggg ttaccgcgcg aacttttcct
      241 taatattggt ttgctggtag gtggattaag ctgcctgaca attccctgtt ttgcctggct
      301 tgccgatcgt tttggtcggc gcagggttta tatcacaggc gcgttgatcg gaacgttgag
      361 cgcatttcct ttctttatgg cgcttgaagc acaatctatt ttctggatag ttttcttctc
      421 cataatgctg gcaaacattg cgcatgacat ggtggtgtgt gtgcaacaac cgatgtttac
      481 cgaaat
//
LOCUS       DAEBXN010000411          485 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.421, whole genome shotgun sequence.
ACCESSION   DAEBXN010000411 DAEBXN010000000
VERSION     DAEBXN010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.421"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            1..485
                     /locus_tag="KJL99_004724"
                     /pseudo
     CDS             1..485
                     /locus_tag="KJL99_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
BASE COUNT          140 a          126 c          124 g           95 t
ORIGIN      
        1 tcagggcaaa acaccaaaag cctcccccat taccccggaa caaattgaaa tccgtgagct
       61 caggaaaaag ctacaacgca ttgaaatgga gaatgaaata ttaaaaaagg ctaccgcgct
      121 cttgatgtca gactccctga acagttctcg ataatcggga aactcagagc gcattatcct
      181 gtggtcacac tctgccatgt gttcggggtt catcgcagca gctacagata ctggaaaaac
      241 cgtcctgaaa aaccagacgg cagacgggct gtattacgca gtcaggtact tgagctacat
      301 ggcatcagcc acggttcggc cggagcaaga agcatcgcca caatggcaac ccggagaggc
      361 taccagatgg gacgctggct tgctggcagg ctcatgaaag agctggggct ggtcagctgt
      421 cagcagccga ctcaccggta taaacgtggt ggtcatgaac atgttgctat ccctaactac
      481 cttga
//
LOCUS       DAEBXN010000412          480 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.422, whole genome shotgun sequence.
ACCESSION   DAEBXN010000412 DAEBXN010000000
VERSION     DAEBXN010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.422"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
BASE COUNT          135 a           98 c           89 g          158 t
ORIGIN      
        1 taacaggatg ctctgactcg caattcatcc agcagtttat catttcccat aatgtcactt
       61 accctcatcg gtaaaaaaat tgcctttcga ccattacgat acattattga ttttggggtt
      121 tcagggaagt aatccatttc gactataact gacaggtcat cacgacgtat gactgcgtat
      181 ttgctactaa atagtttctt tattttttcc acgatgcccc caggtttata agtacaaacg
      241 gttatatcca catagagaca aaaatattaa tctgataaat atttatttca cgccgtatat
      301 ttgattgttt aatgtgcaag tacaatgact tttatttttt gttgtgtata taatcaaata
      361 tatggttatt tttcaccctg cgtattcagc acgcaacaaa aaacccgccg aagcgggttt
      421 agtgcgggtg cgttgaggat gcctgacaca tgagaggtgg cgagggattt ctcccccgcc
//
LOCUS       DAEBXN010000413          468 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.423, whole genome shotgun sequence.
ACCESSION   DAEBXN010000413 DAEBXN010000000
VERSION     DAEBXN010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.423"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..>468
                     /locus_tag="KJL99_004725"
     CDS             <1..>468
                     /locus_tag="KJL99_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4439119.1"
                     /translation="SGSTLISRDNKLVENFIDTLENWRDQKQIISVICKDGIKIDDSI
                     ITSFKASKDVGISNGLRIQLTFQEINFKAIVGQTDVSAATGRTSTTNDGGATSKKNTG
                     NTTTSLGSPMLTCKELFSYSASELSDEALKARVTCSKSVSVKNGESTFTASAN"
BASE COUNT          148 a           96 c          103 g          121 t
ORIGIN      
        1 aatccggtag tacccttatc tcacgagata ataagctggt ggagaacttc atcgacactc
       61 ttgaaaactg gcgtgaccag aagcagatta tttcagttat ctgtaaagat ggtattaaga
      121 ttgacgactc cattattaca agctttaaag cctctaaaga tgttggaatt tcaaacggac
      181 tgagaatcca gctaactttt caggaaatta acttcaaagc tattgtaggg caaactgatg
      241 tttcagcagc cactggacgg acttctacca cgaatgatgg cggtgctacc agtaaaaaga
      301 acactggcaa cactacgaca agtcttggaa gccctatgct gacctgtaaa gagcttttta
      361 gctattcggc aagtgagtta tcagatgaag cgttaaaagc ccgtgtaacg tgctctaaga
      421 gcgttagcgt taaaaatggt gagagcacct tcacagcctc agctaatg
//
LOCUS       DAEBXN010000414          463 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.424, whole genome shotgun sequence.
ACCESSION   DAEBXN010000414 DAEBXN010000000
VERSION     DAEBXN010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.424"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            1..463
                     /locus_tag="KJL99_004726"
                     /pseudo
     CDS             1..463
                     /locus_tag="KJL99_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          109 a          133 c          136 g           85 t
ORIGIN      
        1 gaacgtgaac tggagatgtc taccgagatt gcacgtctca aacgccagct ggcagaacgg
       61 gatgaagagc tggctatcct ccaaaaggcc gcgacatact tcgcgaagcg cctgaaatga
      121 agtatgtctt tattgaaaaa catcaggctg agttcagcat caaagcaatg tgccgcgtgc
      181 tccgggtggc ccgcagcggc tggtatacgt ggtgtcagcg gcggacaagg ataagcacgc
      241 gtcagcagtt ccgccaacac tgcgacagcg ttgtcctcgc ggcttttacc cggtcaaaac
      301 agcgttacgg tgccccacgc ctgacggatg aactgcgtgc tcagggttac ccctttaacg
      361 taaaaaccgt ggcggcaagc ctgcgccgtc agggactgag ggcaaaggcc tcccggaagt
      421 tcagcccggt cagctaccgc gcacacggcc tgcctgtgtc aga
//
LOCUS       DAEBXN010000415          463 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.425, whole genome shotgun sequence.
ACCESSION   DAEBXN010000415 DAEBXN010000000
VERSION     DAEBXN010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.425"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..>463
                     /locus_tag="KJL99_004727"
     CDS             <1..>463
                     /locus_tag="KJL99_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HBD4439120.1"
                     /translation="YASAGLLARILVSKYMEHIPLYRQSEIYARQGVELSRNTMVRWV
                     SEMADKLRPLYIALNDYVLEAGKVHADDTPVKVLAPGNGKTKTGRLWVYVRDDRNAGS
                     SLPAAVWFAYSADRKGEHPQLHLAKYQGVLQADAYAGYNVLYETGRVKEAGC"
BASE COUNT          117 a          109 c          142 g           95 t
ORIGIN      
        1 tatgccagtg cagggttact tgcacggatc ctggtcagca aatatatgga acatatccct
       61 ttatatcgcc agtcagaaat atacgcgcga cagggcgtgg agctgagccg taataccatg
      121 gtgcgctggg tatcagaaat ggcagacaaa ctccgtcctc tgtatatagc gctgaatgac
      181 tatgttctgg aggcaggaaa ggtgcacgca gatgacactc cggtgaaagt actggccccg
      241 gggaacggaa agacgaaaac gggtcgtctg tgggtatacg tcagggatga tcgtaatgcg
      301 ggttcatccc tgccggcagc cgtctggttc gcgtattcgg cagatcgcaa aggagaacat
      361 ccgcagctcc acctggcaaa gtatcagggc gtactgcagg ctgatgccta tgcaggttat
      421 aacgtactgt acgaaacggg ccgggtgaag gaagccgggt gcc
//
LOCUS       DAEBXN010000416          460 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.426, whole genome shotgun sequence.
ACCESSION   DAEBXN010000416 DAEBXN010000000
VERSION     DAEBXN010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.426"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(<1..460)
                     /locus_tag="KJL99_004728"
     CDS             complement(<1..460)
                     /locus_tag="KJL99_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaU family protein"
                     /protein_id="HBD4439121.1"
                     /translation="MLSNRVASYGAIMAALPYMQLYIADYLADTMHLSAEEHGAYLLL
                     MFNYWQTGKPIPKNRLAKIARLTNERWVDVEPSLREFFCDNGDEWMHLRIEEDLASVR
                     EKLTKKSAAGKASVQARRSRKEADVQTKQERNLTGVQTDVGVVFEHDAGFA"
BASE COUNT          106 a          117 c           90 g          147 t
ORIGIN      
        1 gggcaaatcc agcatcatgt tcgaacacca ctccaacatc tgtttgaaca cctgttaaat
       61 ttctctcttg ttttgtttga acatctgctt cctttctgct tcttctggcc tgaacagatg
      121 cttttcctgc ggctgatttt ttggttaatt tttctctgac agatgccaga tcttcctcaa
      181 tccgaagatg catccattcg tcgccgttat cacaaaaaaa ctcccgcaag gatggttcaa
      241 catcaaccca tcgctcgtta gtcagacggg caatttttgc caacctgttt ttgggtattg
      301 gctttcctgt ttgccagtaa ttgaacatca gcaacaaata cgcgccgtgc tcctctgctg
      361 acaaatgcat ggtgtcagcc aggtaatcag ctatgtacag ttgcatgtat ggtaatgcgg
      421 ccataattgc cccgtatgat gctacccggt tgcttagaat
//
LOCUS       DAEBXN010000417          455 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.427, whole genome shotgun sequence.
ACCESSION   DAEBXN010000417 DAEBXN010000000
VERSION     DAEBXN010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.427"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(<1..383)
                     /locus_tag="KJL99_004729"
     CDS             complement(<1..383)
                     /locus_tag="KJL99_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4439122.1"
                     /translation="MVSNTQLLEQRIADFFTLSDEHKKARVLLDTLACSCPARIFGGM
                     VRDLGLYGVDGFSSDLDIVIGRSREELFQTLAELPVKQLRFNKFGGIRFRYHDFEFDI
                     WNLNETWAFQEKLIFCEDESSLLNEV"
BASE COUNT          137 a          109 c           96 g          113 t
ORIGIN      
        1 acctcattta acagacttga ttcatcctca caaaatataa gtttttcctg gaatgcccat
       61 gtctcattga ggttccagat atcaaattcg aagtcgtgat aacgaaaacg gatcccacca
      121 aacttattaa aacgcagttg tttgacgggc agttcagcca atgtctggaa gagttcctca
      181 cgggagcgac cgatgacgat atcaaggtct gaagagaatc catcgacgcc atacaagcct
      241 aagtcacgca ccatcccgcc aaaaatcctg gccggacagg aacaggccag agtatcaagt
      301 aatactctgg ctttcttatg ctcatcacta agcgtaaaaa aatcggcgat acgctgttca
      361 agcagttggg tgtttgagac cacattcata cagtgatttt agctcatcgt gaaggcgcat
      421 taaataacta tccgtcatgc ctgagattgc gtcgg
//
LOCUS       DAEBXN010000418          444 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.428, whole genome shotgun sequence.
ACCESSION   DAEBXN010000418 DAEBXN010000000
VERSION     DAEBXN010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.428"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
BASE COUNT          142 a           87 c           65 g          150 t
ORIGIN      
        1 cgtaccgttt aaacaaagac aatacgtctc tttatttctc ataaaacccc aatcccttac
       61 agggcttatt gaaaaattta aaaatcacaa atttctcaat actcgaaatg agatcacata
      121 agaaatcaaa aagccttctc atctatttta agttttccca acggaaaata agagctttca
      181 ttaatactga accactttac tgttctattt gttcaggtat tgcattacgg tgtgctgaga
      241 tccatcggtg catccctgta tcccgcgatg agactaaaat agtcgtgatg taagctcgta
      301 tcagattctt agtgaatacc acatttgtta taattaagtg aaaagataca ttgctgattt
      361 acattatttg gtttacaaaa tgtcatcact tgcagagtta tcgctgacag atacctttaa
      421 tctcttacta ctttcgtgtt attg
//
LOCUS       DAEBXN010000419          444 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.429, whole genome shotgun sequence.
ACCESSION   DAEBXN010000419 DAEBXN010000000
VERSION     DAEBXN010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.429"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            213..>444
                     /locus_tag="KJL99_004730"
     CDS             213..>444
                     /locus_tag="KJL99_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HBD4439123.1"
                     /translation="MTLPTTIYSFPAYLPRFSSTDKPAKLKFHQYARATLLSNRGRDH
                     NCDSRRTVEIHKLDLSDWQAFNKLATKCNAYDG"
BASE COUNT          141 a          105 c           62 g          136 t
ORIGIN      
        1 tatcgaacct cgcttaccaa cgcgttgtca gtcatctccc ccctctctgg tgcatcgtta
       61 tatccgtgat gagcgatttt tgatattaat atctttcacc atccgctatt ttcaccactt
      121 cagaaaacaa acttattttt attaaaatca attctttttc tttgaacatt caactgcgat
      181 atttgtaaaa acattaaaca ggcaacaata aaatgacatt accaacaact atttattcat
      241 tccccgcata tttaccccgt ttttcgtcca ccgacaaacc agcaaaatta aaatttcatc
      301 aatacgcacg cgctacatta ctgtcaaatc gtggaagaga tcataattgc gatagcagac
      361 gtactgttga gatccacaaa ctagatctct ctgactggca agcattcaat aagttagcaa
      421 cgaagtgtaa tgcttacgat ggag
//
LOCUS       DAEBXN010000420          434 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.430, whole genome shotgun sequence.
ACCESSION   DAEBXN010000420 DAEBXN010000000
VERSION     DAEBXN010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.430"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            35..434
                     /locus_tag="KJL99_004731"
                     /pseudo
     CDS             35..434
                     /locus_tag="KJL99_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          100 a          112 c          114 g          108 t
ORIGIN      
        1 atttagtgta tgatggtgat tttaaggtgc ttgcgtggct tccatttcca tcagatgtcc
       61 ttcctgctcc gctactgaag gcgtggtgcg taacggcaaa agcactgccg gacatcagcg
      121 ctatctctgc tctcattgcc gtaaaacatg gcaactacag ttcacttaca ccgcctctca
      181 gcccggtacg caccagaaaa tcattgatat ggccatgaat ggcgtcggat gtcgcgccag
      241 tgcacgcatt atgggcgttg gcctcaacac ggttttacgt cacttaaaaa actcaggccg
      301 cagtcggtaa cctcgcgcat acaaccgggc agtgatgtga ttgtctgcgc tgaaatggac
      361 gaacattggg gctacgtcgg tgctaaatca cgtcagcgct ggctgtttta cgcgtatgac
      421 aggatacgga ggac
//
LOCUS       DAEBXN010000421          431 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.431, whole genome shotgun sequence.
ACCESSION   DAEBXN010000421 DAEBXN010000000
VERSION     DAEBXN010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.431"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            1..149
                     /locus_tag="KJL99_004732"
                     /pseudo
     CDS             1..149
                     /locus_tag="KJL99_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(169..>431)
                     /locus_tag="KJL99_004733"
     CDS             complement(169..>431)
                     /locus_tag="KJL99_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS4/IS5 family transposase"
                     /protein_id="HBD4439124.1"
                     /translation="STGRILVLSLLATLSTIVMWLLGYHAENKGLHLKYQANSIKSRR
                     VISYLTLAKNVLRHSPLILRRTVLSTVLNHLSRTYRNMVLVY"
BASE COUNT          110 a          104 c          101 g          116 t
ORIGIN      
        1 cgcgcgaagt gttgtatgac aatatgaaaa ctgtggttct gcaacgtgac gcatatcaga
       61 ccggtcagca ccggttccat ccttcgctgt ggcagttcgg caaggagatg ggcttctctc
      121 cccgactgtg tcgccccttc aggctgaggg atccccacaa aattactgct aataaaccag
      181 caccatattt cggtaggttc tggacagatg gtttagaact gtgctcagta ctgttcgtct
      241 taaaataagt ggcgagtgtc gaagaacatt cttcgctaac gtcagatatg agataacccg
      301 ccgggattta atgctgttag cctgatattt caggtgtaac cctttatttt cagcgtgata
      361 accgagtaac cacattacga tagtgctcaa cgttgccagc agactcagaa ccagaattct
      421 tcctgttgaa c
//
LOCUS       DAEBXN010000422          428 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.432, whole genome shotgun sequence.
ACCESSION   DAEBXN010000422 DAEBXN010000000
VERSION     DAEBXN010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.432"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..>428
                     /gene="istA"
                     /locus_tag="KJL99_004734"
     CDS             <1..>428
                     /gene="istA"
                     /locus_tag="KJL99_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS21-like element IS100 family transposase"
                     /protein_id="HBD4439125.1"
                     /translation="SRNTVKRYLQAKSEPPKYTPRPAVASLLDEYRDYIRQRIADAHP
                     YKIPATVIAREIRDQGYRGGMTILRAFIRSLSVPQEQEPAVRFETEPGRQMQVDWGTM
                     RNGRSPLHVFVAVPGYSRMLYIEFTDNMRYDTLETCHRNA"
BASE COUNT          100 a          118 c          105 g          105 t
ORIGIN      
        1 tctcccgcaa taccgttaaa cgttatttgc aggcaaaatc tgagccgcca aaatatacgc
       61 cgcgacctgc tgttgcttca ctcctggatg aataccggga ttatattcgt caacgcatcg
      121 ccgatgctca tccttacaaa atcccggcaa cggtaatcgc tcgcgagatc agagaccagg
      181 gatatcgtgg cggaatgacc attctcaggg cattcattcg ttctctctcg gttcctcagg
      241 agcaggagcc tgccgttcgg ttcgaaactg aacccggacg acagatgcag gttgactggg
      301 gcactatgcg taatggtcgc tcaccgcttc acgtgttcgt tgctgttccc ggatacagcc
      361 gaatgttgta catcgaattc actgacaata tgcgttatga cacgctggag acctgccatc
      421 gtaatgcg
//
LOCUS       DAEBXN010000423          416 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.433, whole genome shotgun sequence.
ACCESSION   DAEBXN010000423 DAEBXN010000000
VERSION     DAEBXN010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.433"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..373
                     /gene="istB"
                     /locus_tag="KJL99_004735"
     CDS             <1..373
                     /gene="istB"
                     /locus_tag="KJL99_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HBD4439126.1"
                     /translation="TAADLLLQLSTAQRQGRYKTTLQRGVMAPRLLIIDEIGYLPFSQ
                     EEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGDAALTSAMLGRILHHSHVVQIK
                     GESYRLRQKRKAGVIAEANPE"
BASE COUNT          121 a           99 c          105 g           91 t
ORIGIN      
        1 aacagcagca gatctgttac ttcagttatc tacggcacaa cgtcagggcc gttataaaac
       61 gacgcttcag cgtggagtaa tggccccccg cctgctcatc attgatgaaa taggctatct
      121 gccgttcagt caggaagaag caaaactgtt cttccaggtc atcgctaaac gttacgaaaa
      181 gagcgcaatg atcctgacat ccaatctacc gttcgggcag tgggatcaaa cgttcgccgg
      241 tgatgcagca ctgacctcag cgatgctggg ccgtatctta caccactcac atgtcgttca
      301 aatcaaagga gaaagctatc gactcagaca gaaacgaaag gccggggtta tagcagaagc
      361 taatcctgag taaaacggtg gatcaatatt gggccgttgg tggagatata agtgga
//
LOCUS       DAEBXN010000424          416 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.434, whole genome shotgun sequence.
ACCESSION   DAEBXN010000424 DAEBXN010000000
VERSION     DAEBXN010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.434"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..224
                     /locus_tag="KJL99_004736"
     CDS             <1..224
                     /locus_tag="KJL99_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HBD4439127.1"
                     /translation="FQRDRYGRILGQVYAPDGMNINQFMVRAGAAWVYEQYNTDPVLP
                     VLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            270..>416
                     /locus_tag="KJL99_004737"
     CDS             270..>416
                     /locus_tag="KJL99_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="with Hns involved in transcriptional regulation of
                     hemolysin; non-specific DNA-binding protein which affects
                     the production of multiple proteins; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBD4439128.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSNSERESFNSAA
                     DHRLA"
BASE COUNT          128 a           91 c           99 g           98 t
ORIGIN      
        1 attttcagcg agatcgttat ggtcgtattc tcgggcaggt ttatgcacca gacggaatga
       61 atattaatca gtttatggtc cgggccgggg ctgcctgggt atatgagcag tacaacacag
      121 atcccgtgct acctgtcctt cagaacgaag ccagacaaca aaaacgtggg ctctggtcag
      181 atgctgatcc ggttcccccc tggatatgga ggcaccgtaa ataaagcgat ggcaatccta
      241 tcattcatct gttctcagcg ggaataaaaa tggcaaaaac aaaacaggaa tggctgtatc
      301 agttacggcg atgttcatca gtcaatacgc tggaaaaaat tattcataaa aacagagaca
      361 gtttatcaaa cagtgaacgt gaatcattta attcagcagc agaccaccgg ctagcg
//
LOCUS       DAEBXN010000425          415 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.435, whole genome shotgun sequence.
ACCESSION   DAEBXN010000425 DAEBXN010000000
VERSION     DAEBXN010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.435"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(<1..259)
                     /locus_tag="KJL99_004738"
     CDS             complement(<1..259)
                     /locus_tag="KJL99_004738"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4439129.1"
                     /translation="MARSAKPRKRKPAPQRSKLLRYVVKLHEDDFFDEEEAEVLRFDN
                     FDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEGYPEIARC"
BASE COUNT          114 a           94 c           93 g          114 t
ORIGIN      
        1 tgcagcgggc aatttccgga tacccttcgt catcaacacg tacaaaccag aagaccagtt
       61 ttttgtttcc ggcatccaca aagaacggaa tattcaggtc tgcgcaacac tcaacggcat
      121 cgtcaaaatt atcaaagcgc agaacctcag cttcttcttc gtcaaaaaaa tcatcttcgt
      181 gaagttttac gacatagcgg aggagtttgc ttctttgagg tgcaggtttt cgtttgcgtg
      241 gtttagctga acgggccata taactacctg aaagacaatg atattgtctg tttttataac
      301 ggtaatcaca ggccatgaca agccgcagcc gtcaggccgc ctactcgggg cggtttagaa
      361 cgcttattta gtaccagatc ttacttataa taaaggggct tggcagtttg gataa
//
LOCUS       DAEBXN010000426          390 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.436, whole genome shotgun sequence.
ACCESSION   DAEBXN010000426 DAEBXN010000000
VERSION     DAEBXN010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.436"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(42..>390)
                     /locus_tag="KJL99_004739"
     CDS             complement(42..>390)
                     /locus_tag="KJL99_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBD4439130.1"
                     /translation="QIKPFLEKAMTMYNDVLFPGEDINKPISIAAANRFVNRIRGGMD
                     LGYWRTHDFRRTLVTRLSEMNVEPHVTERMLGHELGGIMSVYNKHDWIEAQRKAYELH
                     ADKLFWHIRSISD"
BASE COUNT           92 a           91 c           87 g          120 t
ORIGIN      
        1 ccttcgtgaa gctgttgaag ggtggattct taacggtggc gttaatcaga aatgctcctg
       61 atgtgccaga acaatttatc agcgtgaagc tcatacgctt tgcgctgagc ctctatccag
      121 tcgtgtttat tgtatacgga cattatcccg ccaagttcat gaccgagcat tcgctcagta
      181 acatgaggct cgacattcat ctcggacagc cgtgtaacaa gcgttcttct gaaatcatgt
      241 gttcgccagt aacctagatc catccctccc cttattctat ttacgaatcg gttggctgca
      301 gcgatgctga tcggtttgtt tatgtcttct ccagggaaaa gaacatcatt gtacattgtc
      361 atggcctttt cgagaaaagg cttaatttgt
//
LOCUS       DAEBXN010000427          380 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.437, whole genome shotgun sequence.
ACCESSION   DAEBXN010000427 DAEBXN010000000
VERSION     DAEBXN010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.437"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(1..248)
                     /locus_tag="KJL99_004740"
                     /pseudo
     CDS             complement(1..248)
                     /locus_tag="KJL99_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT           87 a          121 c           86 g           86 t
ORIGIN      
        1 ctgtctccgt tatggcgtac accacatctt ccagtaatcg ccgcttcacc ggactcagga
       61 ccccgtatca ccatccggaa ccccttatcc gccacatgcc aggagaacct ttccataaac
      121 tcgtcgccac tcatcacaag gttttcttct cgttttgtcc ggtggctgtt gtaacgcagg
      181 ccaatttcat cctgaccggc ataatgttcc aggcggctca tctgcaccgg ctgcttctcc
      241 agccagttaa gccatcggtt tccctgctga aaaatatcgg caaaacgggg aagcatcaga
      301 aaggcggaat aacgccatcc ggccagtgaa ccgtaccgca ttcagggcaa tacatgccgt
      361 cggcgttgat accggcagga
//
LOCUS       DAEBXN010000428          375 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.438, whole genome shotgun sequence.
ACCESSION   DAEBXN010000428 DAEBXN010000000
VERSION     DAEBXN010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.438"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..>375
                     /locus_tag="KJL99_004741"
     CDS             <1..>375
                     /locus_tag="KJL99_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBD4439131.1"
                     /translation="DGRRSRHSDDTDVLLRIHHVIGELPTYGYRRVWALLRRQAELDG
                     MPAINAKRIYRLMRQNALLLERKLAVPPSKRAHTGRVAVKESNQRWCSDGFEFRCDNG
                     EKLRVTFALDCCDRKALHWAVTT"
BASE COUNT           86 a           96 c          110 g           83 t
ORIGIN      
        1 agatggtcgc cgcagccgtc actcagatga tacggatgtg cttctccgta tacaccatgt
       61 tatcggagag ctgcccacat atggttatcg tcgggtatgg gcgctgcttc gcagacaggc
      121 cgaacttgat ggtatgcctg cgatcaatgc caaacgtatt taccggctca tgcgccagaa
      181 tgcgctgttg cttgagcgaa aactcgctgt accgccatcg aaacgggcac ataccggcag
      241 agtggctgtg aaagaaagta atcagcgatg gtgctctgac gggttcgagt tccgctgtga
      301 taacggagaa aaactgcgag tcacgttcgc gctggactgc tgtgaccgta aggcactgca
      361 ctgggcggtc actac
//
LOCUS       DAEBXN010000429          372 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.439, whole genome shotgun sequence.
ACCESSION   DAEBXN010000429 DAEBXN010000000
VERSION     DAEBXN010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.439"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(<1..311)
                     /locus_tag="KJL99_004742"
     CDS             complement(<1..311)
                     /locus_tag="KJL99_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBD4439132.1"
                     /translation="MNSQTTKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFLASGIAWPLPDSVSFAQLDAILYANRKKELTEPQIREGSWRKERRTSYSRE
                     F"
BASE COUNT           99 a           74 c           96 g          103 t
ORIGIN      
        1 ttaaattcac ggctatagct ggttcgccgt tcttttcgcc atgagccttc tctgatttga
       61 ggctctgtta attccttctt tctgttggca taaaggatgg cgtcaagttg agcgaatgaa
      121 actgaatcgg gcaatggcca tgcgataccg gatgcaagaa atcgctgaaa aagcgtatgt
      181 attgtggaat gactgagacc cagacgctga gcgatggccc ggatggtcag tttatcttca
      241 aatcttaaac gcagggcatc aggcaaataa gaacggaagc agggaatatc ttttgttgtc
      301 tgggaattca tcgttcgtgt ccatcaatat agatgggcgc gattgttgcc agacaggaca
      361 attttcacaa ga
//
LOCUS       DAEBXN010000430          365 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.440, whole genome shotgun sequence.
ACCESSION   DAEBXN010000430 DAEBXN010000000
VERSION     DAEBXN010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.440"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..>365
                     /locus_tag="KJL99_004743"
     CDS             <1..>365
                     /locus_tag="KJL99_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HBD4439133.1"
                     /translation="HQIVNRLDIMLPGNRPFVAPSAVIQARQRLGSEAVRRVFTKTAQ
                     LWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTHAGNPALYPQVKMVCQME
                     LTSHLLTAAAFGTMKNSEN"
BASE COUNT           76 a          124 c          102 g           63 t
ORIGIN      
        1 tcaccagatt gtgaatcgcc tggacatcat gctgccgggc aatcgcccct tcgttgcccc
       61 cagtgccgtt attcaggccc gccagcgcct gggaagtgag gctgtccgcc gcgtgttcac
      121 gaaaacagcg cagctctggc ataacgccac gccgcatccg cactggtgcg gcctgaccct
      181 gctggccatc gatggtgtgt tctggcgcac accggataca ccagagaacg atgcagcctt
      241 cccccgccag acacatgccg ggaacccggc gctctacccg caggtcaaaa tggtctgcca
      301 gatggaactg accagccatc tgctgacggc tgcagccttc ggcacgatga agaacagcga
      361 aaatg
//
LOCUS       DAEBXN010000431          359 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.441, whole genome shotgun sequence.
ACCESSION   DAEBXN010000431 DAEBXN010000000
VERSION     DAEBXN010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.441"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
BASE COUNT           76 a          102 c           90 g           91 t
ORIGIN      
        1 agcctgcgct gatgcagcat gtccctgaag gcgtgattca ggcaccagaa tcacccgacg
       61 ccccggaggc gctgccgggg tgtcgttcca tttctgatga tgacctgtgc gcctggtgta
      121 cccgtctgct ttaccgtccc ggagaaatca gtctgtgcag actgagtata caggcaatgg
      181 catctggcca tccgtctgtg accggaacgg ttacgcgtac ggctgtccgg aatttcagcc
      241 gaatatcatt catccctgat attaccgggt catcacggtc agtgatgaac gtgatgttgc
      301 cagctcccga cctttactcc ctctcattta ttcacattac aaggatttca tacatgaaa
//
LOCUS       DAEBXN010000432          357 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.442, whole genome shotgun sequence.
ACCESSION   DAEBXN010000432 DAEBXN010000000
VERSION     DAEBXN010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..357
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.442"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            39..>357
                     /locus_tag="KJL99_004744"
     CDS             39..>357
                     /locus_tag="KJL99_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HBD4439134.1"
                     /translation="MNNELPDDIELLKAMLRKQQSRLRQYACQVAGYEQEIERLKAQL
                     DRLRRMLFGQSSEKKRHKLENQIRQAEKRLSELENRLNTARNLLEDASSVTDSPDTSP
                     PSEN"
BASE COUNT          112 a           86 c           91 g           68 t
ORIGIN      
        1 gcctggcatc cggatataac ccgtgataaa acaagggaat gaacaatgaa ctccccgatg
       61 atattgagct gcttaaagcc atgttgcgta agcaacagag tcggcttcga cagtatgcct
      121 gtcaggtcgc gggctatgag caggaaattg aacggctgaa agcgcaactc gacaggttgc
      181 gtcgtatgtt gttcggccag agttcagaga aaaagcgtca taagcttgaa aatcagatcc
      241 gacaggcaga aaaacgactg tcggaactgg aaaaccggct gaacacagcc agaaatcttc
      301 tggaagatgc atcgtcagtc acagattcac ctgacaccag tcccccgtca gaaaacc
//
LOCUS       DAEBXN010000433          354 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.443, whole genome shotgun sequence.
ACCESSION   DAEBXN010000433 DAEBXN010000000
VERSION     DAEBXN010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.443"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            118..>354
                     /locus_tag="KJL99_004745"
     CDS             118..>354
                     /locus_tag="KJL99_004745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HBD4439135.1"
                     /translation="MRKYIPLALFIFSWPVLSADIHGRVVRVLDGDTIEVMDSLKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVT"
BASE COUNT          100 a           57 c           93 g          104 t
ORIGIN      
        1 taagtcgatt ttttatattt tgtgattagt ttcacaaaat aaggtgttat tcaatgtgtg
       61 ctgcactatt caggggggct gaaatacagg tggcatttat ctgatatgga gaataacatg
      121 aggaaatata ttccactggc attatttatc ttttcatggc cggtattgtc tgcagatatt
      181 catggacggg ttgttcgtgt actggacggt gataccatcg aggtcatgga ctcactgaag
      241 gcggtgcgga tcaggctggt aaatattgat gcaccggaaa agaaacagga ttatggccgg
      301 tggtcaacag acatgatgaa atcactggtc gcaggaaaaa cggttaccgt caca
//
LOCUS       DAEBXN010000434          352 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.444, whole genome shotgun sequence.
ACCESSION   DAEBXN010000434 DAEBXN010000000
VERSION     DAEBXN010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.444"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(109..303)
                     /locus_tag="KJL99_004746"
     CDS             complement(109..303)
                     /locus_tag="KJL99_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004992548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HBD4439136.1"
                     /translation="MLARKGVETVLRLLDKETQELLQLEGRKRLAALMLVDMPDEQVR
                     EILEEIEASILSESVNAKAG"
BASE COUNT           92 a           90 c           65 g          105 t
ORIGIN      
        1 ttcaagatag gcgcgccgtg tattttaatt cttttaagac tcttcaaaaa tgtcatcgta
       61 gcctttaaaa acgaatttta aaggcaccct aaaggcaggt caatgaagtt aacccgcctt
      121 cgcgtttaca ctctctgaga gaatactagc ttcaatctct tccaaaatct ctctgacttg
      181 ctcgtcgggc atatcaacga gcatcaacgc tgccaggcgt ttacggccct ctagttgcag
      241 tagctcttga gtttccttat ctaacaagcg tagcacagtc tcaacacctt tccttgcgag
      301 catgtttgta agggcttctc gttcctcact ggtaagaaac gacaacattt ct
//
LOCUS       DAEBXN010000435          349 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.445, whole genome shotgun sequence.
ACCESSION   DAEBXN010000435 DAEBXN010000000
VERSION     DAEBXN010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.445"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..>349
                     /locus_tag="KJL99_004747"
     CDS             <1..>349
                     /locus_tag="KJL99_004747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HBD4439137.1"
                     /translation="LERKPAVPPSKRAHTGRVAVKESNQRWCSDGFEFCCDNGERLRV
                     TFALDCCDREALHWAVTTGGFNSETVQDVMLGAVERRFGNDLPSSPVEWLTDNGSCYR
                     ANETRQFARMLGL"
BASE COUNT           78 a           85 c          114 g           72 t
ORIGIN      
        1 tgcttgagcg aaaacctgct gtaccgccat cgaaacgggc acatacaggc agagtggccg
       61 tgaaagaaag caatcagcga tggtgctctg acgggttcga gttctgctgt gataacggag
      121 agagactgcg tgtcacgttc gcgctggact gctgtgatcg tgaggcactg cactgggcgg
      181 tgactaccgg cggcttcaac agtgaaacag tacaggacgt catgctggga gcggtggaac
      241 gccgcttcgg caacgatctt ccgtcgtctc cagtggagtg gctgacggat aatggttcat
      301 gctaccgggc taatgaaaca cgccagttcg cccggatgtt gggacttga
//
LOCUS       DAEBXN010000436          344 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.446, whole genome shotgun sequence.
ACCESSION   DAEBXN010000436 DAEBXN010000000
VERSION     DAEBXN010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.446"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..>344
                     /gene="tuf"
                     /locus_tag="KJL99_004748"
     CDS             <1..>344
                     /gene="tuf"
                     /locus_tag="KJL99_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBD4439138.1"
                     /translation="AHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHIL
                     LGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKAL
                     EGDAEWEAKILEL"
BASE COUNT           77 a           88 c          105 g           74 t
ORIGIN      
        1 gcacacgtag actgcccggg gcacgccgac tatgttaaaa acatgatcac cggtgctgct
       61 cagatggacg gcgcgatcct ggtagttgct gcgactgacg gcccgatgcc gcagactcgt
      121 gagcacatcc tgctgggtcg tcaggtaggc gttccgtaca tcatcgtgtt cctgaacaaa
      181 tgcgacatgg ttgatgacga agagctgctg gaactggttg aaatggaagt tcgtgaactt
      241 ctgtctcagt acgacttccc gggcgacgac actccgatcg ttcgtggttc tgctctgaaa
      301 gcgctggaag gcgacgcaga gtgggaagcg aaaatcctgg aact
//
LOCUS       DAEBXN010000437          341 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.447, whole genome shotgun sequence.
ACCESSION   DAEBXN010000437 DAEBXN010000000
VERSION     DAEBXN010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.447"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            4..171
                     /gene="xisR"
                     /locus_tag="KJL99_004749"
     CDS             4..171
                     /gene="xisR"
                     /locus_tag="KJL99_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014884030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HBD4439139.1"
                     /translation="MLRTGLGARQIEAYRQNCWVEGFHFKRVSPLGKPDSKRGIIWYN
                     YPKINQFIKDS"
     gene            173..>341
                     /locus_tag="KJL99_004750"
     CDS             173..>341
                     /locus_tag="KJL99_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HBD4439140.1"
                     /translation="MSKLPTGVEIRGKYIRIWFMFRGKRCRETLKGWEITNSNIKKAG
                     NLRALIVHQISS"
BASE COUNT          108 a           64 c           83 g           86 t
ORIGIN      
        1 ctgatgcttc gcactggtct gggtgccaga caaattgaag cataccgcca gaactgttgg
       61 gtggaaggct tccacttcaa acgagtatct cctttaggga agccagacag taagcgaggg
      121 attatctggt acaactatcc gaagataaat cagtttatca aagactcatg atatgtctaa
      181 attaccaaca ggtgtcgaga ttcgaggtaa atacattcgc atctggttca tgtttcgagg
      241 aaaacgatgt cgggaaacat tgaaaggctg ggagattact aacagtaaca ttaaaaaggc
      301 cgggaattta agagcgttga tagttcatca aatcagttcc g
//
LOCUS       DAEBXN010000438          335 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.448, whole genome shotgun sequence.
ACCESSION   DAEBXN010000438 DAEBXN010000000
VERSION     DAEBXN010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.448"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            136..335
                     /locus_tag="KJL99_004751"
                     /pseudo
     CDS             136..335
                     /locus_tag="KJL99_004751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
BASE COUNT           93 a           81 c           58 g          103 t
ORIGIN      
        1 agacagctac tataactttc atctatttat tcacagcgcg aatgaaaaat aaaacactct
       61 gctttacagt gtttacttta taattaacag tcttatctca ctgttcaccc tgtaacgttc
      121 agagagcgtc ttcagatgtc tatttataaa attcctcttc cgctcaatat tctggaagct
      181 gcacgagagc gtattaccta gacactcaac accctacccc gcgtttgtgt ctcattttct
      241 ggcggcaaag actctggttt aatgctgcat ctgacagccg aacttgcccg acaaatgggc
      301 aaaaaaatct gcgttttgtt tatcaactgg gaggc
//
LOCUS       DAEBXN010000439          335 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.449, whole genome shotgun sequence.
ACCESSION   DAEBXN010000439 DAEBXN010000000
VERSION     DAEBXN010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.449"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..>335
                     /locus_tag="KJL99_004752"
     CDS             <1..>335
                     /locus_tag="KJL99_004752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBD4439141.1"
                     /translation="NGKVIAPACTLVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVP
                     IALEGCDVTVTKNATFTFSGTADGVQPTAFANQATTDAATNVALQMYLPDGSTSVTPG
                     TETSNIQLA"
BASE COUNT           92 a           79 c           78 g           86 t
ORIGIN      
        1 taatggtaaa gttattgctc cagcttgtac cctggtagct gcgacgaaag attcagtagt
       61 cacattgcca aatgtcagcg ccaccaagtt gcaaactaat ggagcagtct ctggcgttaa
      121 aactgatgta ccaattgcct tagaaggctg tgatgttact gtaactaaaa atgcgacgtt
      181 tacctttagt ggtacggctg acggcgtgca acctacagca tttgccaacc aggctaccac
      241 cgatgcagcg acgaatgttg ctctacaaat gtatctgcca gatggttcta catcggtaac
      301 tcccggtacc gaaacgtcga atattcagtt agcgg
//
LOCUS       DAEBXN010000440          333 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.450, whole genome shotgun sequence.
ACCESSION   DAEBXN010000440 DAEBXN010000000
VERSION     DAEBXN010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.450"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(43..330)
                     /locus_tag="KJL99_004753"
     CDS             complement(43..330)
                     /locus_tag="KJL99_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4439142.1"
                     /translation="MDEEYRKDLQLWFGLTHASFCVMPRVFMEAMPPEWQEKMAQLLF
                     EYSDTIKTDVCGVHSCFVTAKDGNNRFMRMPEDILNYRHPRREFIESFLKK"
BASE COUNT           91 a           85 c           67 g           90 t
ORIGIN      
        1 taagccatta tcaaacccac ccgtagatag gctttgtaat ggctacttct tcagaaatga
       61 ttcgatgaat tcacgtcgag gatgacgata gttcagaata tcttctggca tcctcataaa
      121 gcggttgttg ccgtctttgg cagtaacaaa acagctgtga actccgcaga catccgtttt
      181 gatcgtgtcg ctatactcaa aaagcaactg agccatcttc tcttgccatt ctggaggcat
      241 agcctccatg aatactcgcg gcatcacgca gaacgacgca tgcgtaagac caaaccacag
      301 ttgcaggtct ttacgatatt cttcatccat cgt
//
LOCUS       DAEBXN010000441          332 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.451, whole genome shotgun sequence.
ACCESSION   DAEBXN010000441 DAEBXN010000000
VERSION     DAEBXN010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.451"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(<1..>332)
                     /locus_tag="KJL99_004754"
     CDS             complement(<1..>332)
                     /locus_tag="KJL99_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBD4439143.1"
                     /translation="DNLGLKSAATMEAQSDIYDRTKGRLAIPGAFGFGCAFLPEDVIR
                     FDTKSDFLAWVRNALPGEYSVAGPYGIIIPDTRFEGVLSIRWTDARPETTEPRYRAKS
                     LTFYGING"
BASE COUNT           75 a           95 c           85 g           77 t
ORIGIN      
        1 gccgttaatg ccgtaaaaag taagggattt ggctctgtac cgcggttctg ttgtctcagg
       61 gcgtgcatca gtccaccgga tgctgagcac cccttcaaac cgtgtgtcgg gtatgatgat
      121 gccgtagggg ccagcaacgg aatattcacc tggcagcgca ttccttaccc aggccaggaa
      181 atcactctta gtgtcaaaac ggataacatc ttcaggcaga aaagcacacc caaagccgaa
      241 tgcgccgggt atcgccagac ggccttttgt ccggtcgtaa atgtcgctct gtgcttccat
      301 cgtggccgca cttttcagcc ccagattatc cc
//
LOCUS       DAEBXN010000442          325 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.452, whole genome shotgun sequence.
ACCESSION   DAEBXN010000442 DAEBXN010000000
VERSION     DAEBXN010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.452"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(41..99)
                     /locus_tag="KJL99_004755"
                     /pseudo
     CDS             complement(41..99)
                     /locus_tag="KJL99_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
BASE COUNT           73 a           96 c           75 g           81 t
ORIGIN      
        1 acaagggtga gggaggattt ctcccccctc tgattggctg ttaataagct gcgaaactta
       61 cgagtaacaa ccaaccagta tgatgacgaa cttcatcata accctttcct tctataaggc
      121 cccttcctcg ggaggggctt tcccgtttca gccccttgct gactccccga ttgttgatga
      181 gcgccggacc ccgcgcaacg ctttccctga agcagttttg ccttccccga gcggtcacgc
      241 aaaacgctgc aacgtcctga gcatttgcac cttttagcgg aacgcagata taaaaaatcc
      301 ccggcttttt gggccaggga tatgt
//
LOCUS       DAEBXN010000443          324 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.453, whole genome shotgun sequence.
ACCESSION   DAEBXN010000443 DAEBXN010000000
VERSION     DAEBXN010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..324
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.453"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            102..>324
                     /locus_tag="KJL99_004756"
     CDS             102..>324
                     /locus_tag="KJL99_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4439144.1"
                     /translation="MIYSNQLRYTGKDGVYFHLQGQPDNFLSLSATENMEFDSPMMVT
                     TQNMQSGQTVTDNVMRSPRTISISGVVVVG"
BASE COUNT          109 a           56 c           61 g           98 t
ORIGIN      
        1 aatttacgta gctcacgctt aacagagaaa atacttatat atcaaaggct tggttaaaaa
       61 tgattaaatg cgctaagtct ttgaaatcaa aagagaattt aatgatttac tctaatcagc
      121 ttcgttacac tggcaaagat ggtgtttact tccatctaca aggccaacca gataatttct
      181 tgtcactatc agcaactgaa aacatggaat ttgatagccc tatgatggtt accactcaaa
      241 atatgcaatc agggcagact gtaacagata atgtaatgcg ttcacctaga actatttcaa
      301 tcagtggtgt ggttgtggtt ggtg
//
LOCUS       DAEBXN010000444          317 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.454, whole genome shotgun sequence.
ACCESSION   DAEBXN010000444 DAEBXN010000000
VERSION     DAEBXN010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.454"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
BASE COUNT          102 a           56 c           77 g           82 t
ORIGIN      
        1 ttaagtgata ctggttgtct ggagattcag ggggccagtc tagcagttga cggggtatct
       61 gctgcgctgg ccatgagagg aagagcggcg gcgacagcaa caacaagacg tttgggtgaa
      121 aacgaaaatt ccatgcaaaa tgctccggtt tcatgtcatc aaaatgatga cgtaattaag
      181 cattgataat tgagatccct ctccctgaca ggatgattac ataaataata gtgacaaaaa
      241 taaattattt atttatccag aaaatgaatt ggaaaatcag gagagcgttt tcaatcctac
      301 ctctggcgca gttgata
//
LOCUS       DAEBXN010000445          316 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.455, whole genome shotgun sequence.
ACCESSION   DAEBXN010000445 DAEBXN010000000
VERSION     DAEBXN010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..316
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.455"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(<1..>316)
                     /locus_tag="KJL99_004757"
     CDS             complement(<1..>316)
                     /locus_tag="KJL99_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HBD4439145.1"
                     /translation="FAVNGRKLYLSPVIDLFNNEVISYSLSERPVMNMVENMLDQAFK
                     KLNPHEHPVLHSDQGWQYRMRRYQNILKEHGIKQSMSRKGNCLDNAVVECFFGTLKSE
                     CFY"
BASE COUNT           93 a           70 c           57 g           96 t
ORIGIN      
        1 ataaaaacac tccgacttta aggttccaaa gaaacactcc accacagcat tatccagaca
       61 attgcctttt ctggacatgc tttgtttaat accatgttct ttaaggatat tttgatatct
      121 tctcatacga tactgccatc cctggtcaga gtgcagaaca ggatgctcgt gaggattaag
      181 ctttttgaat gcctgatcga gcatattctc aaccatgttc atcactggtc tttccgaaag
      241 gctgtaagaa ataacttcgt tgttgaagag atctattact ggagacaaat acagcttgcg
      301 cccattgact gcaaat
//
LOCUS       DAEBXN010000446          302 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.456, whole genome shotgun sequence.
ACCESSION   DAEBXN010000446 DAEBXN010000000
VERSION     DAEBXN010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.456"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            62..>302
                     /locus_tag="KJL99_004758"
     CDS             62..>302
                     /locus_tag="KJL99_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HBD4439146.1"
                     /translation="MAELAIELDDMTIITEKLRDMKNALSKLNHDIEEDTKRIKAITT
                     GSLKDIDRTTKEGMVETQLLIKGVLKEIVIDAAKRR"
BASE COUNT          117 a           55 c           64 g           66 t
ORIGIN      
        1 tactgacact attgatagcc ttaaattaca gcgtgaagat ttgcagcgta aaatctcaaa
       61 aatggcagaa cttgctattg aactcgatga tatgacaatt atcacagaga aattacgcga
      121 catgaagaat gcgctttcaa aactcaatca tgatatcgaa gaggatacaa agcggataaa
      181 ggccatcaca actggaagcc tcaaagatat tgacaggaca acaaaggaag ggatggtaga
      241 gacacagcta cttatcaagg gcgtactcaa agaaatagtt atagatgccg ctaaacgtcg
      301 at
//
LOCUS       DAEBXN010000447          297 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.457, whole genome shotgun sequence.
ACCESSION   DAEBXN010000447 DAEBXN010000000
VERSION     DAEBXN010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..297
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.457"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            26..235
                     /gene="fumD"
                     /locus_tag="KJL99_004759"
     CDS             26..235
                     /gene="fumD"
                     /locus_tag="KJL99_004759"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HBD4439147.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT           94 a           52 c           82 g           69 t
ORIGIN      
        1 ttggcatgat aaataggtat aaagaatggg aaatcggaca aaagaggatg aactgtaccg
       61 agaaatgtgc agagttgtcg gtaaagttgt gctggaaatg cgcgatctgg ggcaggaacc
      121 aaagcatatt gttatcgcag gcgtattacg tacagcatta gccaataaac gcattcagcg
      181 cagtgagctg gaaaagcagg cgatggaaac cgtcattaac gcactggtaa aatgaccatt
      241 caggatgagg cggtaagctt catcctgata ttcttgcttc agttgcggtc cagttaa
//
LOCUS       DAEBXN010000448          294 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.denovo.458, whole genome shotgun sequence.
ACCESSION   DAEBXN010000448 DAEBXN010000000
VERSION     DAEBXN010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..294
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.denovo.458"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(<1..>294)
                     /locus_tag="KJL99_004760"
     rRNA            complement(<1..>294)
                     /locus_tag="KJL99_004760"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           53 a          102 c           64 g           75 t
ORIGIN      
        1 aatcctgttt gctccccacg ctttcgcacc tgagcgtcag tcttcgtcca gggggccgcc
       61 ttcgccaccg gtattcctcc agatctctac gcatttcacc gctacacctg gaattctacc
      121 cccctctacg agactcaagc ttgccagtat cagatgcagt tcccaggttg agcccgggga
      181 tttcacatct gacttaacaa accgcctgcg tgcgctttac gcccagtaat tccgattaac
      241 gcttgcaccc tccgtattac cgcggctgct ggcacggagt tagccggtgc ttct
//
LOCUS       DAEBXN010000449         6456 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.guided.176, whole genome shotgun sequence.
ACCESSION   DAEBXN010000449 DAEBXN010000000
VERSION     DAEBXN010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6456
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.guided.176"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            42..389
                     /locus_tag="KJL99_004761"
     CDS             42..389
                     /locus_tag="KJL99_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="HBD4439148.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     gene            383..1222
                     /gene="sul1"
                     /locus_tag="KJL99_004762"
     CDS             383..1222
                     /gene="sul1"
                     /locus_tag="KJL99_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /protein_id="HBD4439149.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     gene            1350..1553
                     /locus_tag="KJL99_004763"
     CDS             1350..1553
                     /locus_tag="KJL99_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD4439150.1"
                     /translation="MDSEEPPNVRVACSGDIDEVVRLMHDAAAWMSAKGTPAWDVARI
                     DRTFAETFVLRSELLGIASENGK"
     gene            1709..2914
                     /gene="chrA"
                     /locus_tag="KJL99_004764"
     CDS             1709..2914
                     /gene="chrA"
                     /locus_tag="KJL99_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004896675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HBD4439151.1"
                     /translation="MTNNPTDDSRPWSVFLIFLRLGLTSFGGPIAHLGYFRAEFVTRR
                     RWLSERSYADLVALCQFLPGPASSQVGIAVGLSRAGYSGALAAWAGFTLPSAIALILF
                     ALGISSYGDYVSQGALHGLKVVAVAVVAQAVWGMARNLCTDGLRVTIMAIATCVVLLV
                     PSAWGQVGVIAIAGIAGRLLFKPAKVVEHDPLPITVSHRAGVLWLSLFFVLLIGLPVL
                     AELMPSQTMAMVDSFYRVGSLVFGGGHVVLPLLQAEVVPSGWVNNESFLAGYGAAQAV
                     PGPLFTFAAFLGASMNTAPSGWIGGIVCLLAIFAPSFLLVVGSMPFWERLRRNTGIQA
                     ALAGINAAVVGLLLAALYQPVWTSAIFQPQDFGLALVALVALMFWKLPPWLVVLGSGA
                     AGWLLSVAL"
     gene            2925..3230
                     /locus_tag="KJL99_004765"
     CDS             2925..3230
                     /locus_tag="KJL99_004765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBD4439152.1"
                     /translation="MTDKDLYGGLIRLHILHHAAEEPVFGLGIIEELRRHGYEMSAGT
                     VYPMLHGLEKKGYLTSRHERTGRRERRVYDITEQGRTALADAKTKVKELFGELVEGG"
     gene            3274..3339
                     /locus_tag="KJL99_004766"
                     /pseudo
     CDS             3274..3339
                     /locus_tag="KJL99_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229646.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            3457..4221
                     /locus_tag="KJL99_004767"
     CDS             3457..4221
                     /locus_tag="KJL99_004767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS6100 family transposase"
                     /protein_id="HBD4439153.1"
                     /translation="MTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEMLAERGISVDH
                     TTIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYRAVDKRGDTID
                     FYLSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELKREGKLDRETA
                     HRQVKYLNNVIEADHGKLKILIKPVRGFKSIPTAYATIKGFEVMRALRKGQARPWCLQ
                     PGIRGEVRLVERAFGIGPSALTEAMGMLNHHFAAAA"
     gene            complement(4714..5298)
                     /locus_tag="KJL99_004768"
     CDS             complement(4714..5298)
                     /locus_tag="KJL99_004768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBD4439154.1"
                     /translation="MPRPKLKSDDEVLEAATVVLKRCGPIEFTLSGVAKEVGLSRAAL
                     IQRFTNRDTLLVRMMERGVEQVRHYLNAIPIGAGPQGLWEFLQVLVRSMNTRNDFSVN
                     YLISWYELQVPELRTLAIQRNRAVVEGIRKRLPPGAPAAAELLLHSVIAGATMQWAVD
                     PDGELADHVLAQIAAILCLMFPEHDDFQLLQAHA"
     gene            complement(5298..>6456)
                     /locus_tag="KJL99_004769"
     CDS             complement(5298..>6456)
                     /locus_tag="KJL99_004769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD4439155.1"
                     /translation="LALPWFVLSHTKSAAWAGATAASSVIATIIGAWVGGGLVDRFGR
                     APVALISGVVGGVAMASIPLLDAVGALSNTGLIACVVLGAAFDAPGMAAQDSELPKLG
                     HVAGLSVERVSSLKAVIGNVAILGGPALGGAAIGLLGAAPTLGLTAFCSVLAGLLGAW
                     VLPARAARTMTTTATLSMRAGVAFLWSEPLLRPLFGIVMIFVGIVGANGSVIMPALFV
                     DAGRQVAELGLFSSMMGAGGLLGIAIHASVGARISAQNWLAVAFCGSAVGSLLLSQLP
                     GVPVLMLLGALVGLLTGSVSPILNAAIYNRTPPELLGRVLGTVSAVMLSASPMVMLAA
                     GAFVDLAGPLPGLVVSAVFAGLVALLSLRLQFATMAAAATASAPTHTEGEH"
BASE COUNT         1295 a         1883 c         1948 g         1330 t
ORIGIN      
        1 aagcgtgcat aataagccct acacaaattg ggagatatat catgaaaggc tggctttttc
       61 ttgttatcgc aatagttggc gaagtaatcg caacatccgc attaaaatct agcgagggct
      121 ttactaagct tgccccttcc gccgttgtca taatcggtta tggcatcgca ttttattttc
      181 tttctctggt tctgaaatcc atccctgtcg gtgttgctta tgcagtctgg tcgggactcg
      241 gcgtcgtcat aattacagcc attgcctggt tgcttcatgg gcaaaagctt gatgcgtggg
      301 gctttgtagg tatggggctc ataattgctg cctttttgct cgcccgatcc ccatcgtgga
      361 agtcgctgcg gaggccgacg ccatggtgac ggtgttcggc attctgaatc tcaccgagga
      421 ctccttcttc gatgagagcc ggcggctaga ccccgccggc gctgtcaccg cggcgatcga
      481 aatgctgcga gtcggatcag acgtcgtgga tgtcggaccg gccgccagcc atccggacgc
      541 gaggcctgta tcgccggccg atgagatcag acgtattgcg ccgctcttag acgccctgtc
      601 cgatcagatg caccgtgttt caatcgacag cttccaaccg gaaacccagc gctatgcgct
      661 caagcgcggc gtgggctacc tgaacgatat ccaaggattt cctgaccctg cgctctatcc
      721 cgatattgct gaggcggact gcaggctggt ggttatgcac tcagcgcagc gggatggcat
      781 cgccacccgc accggtcacc ttcgacccga agacgcgctc gacgagattg tgcggttctt
      841 cgaggcgcgg gtttccgcct tgcgacggag cggggtcgct gccgaccggc tcatcctcga
      901 tccggggatg ggatttttct tgagccccgc accggaaaca tcgctgcacg tgctgtcgaa
      961 ccttcaaaag ctgaagtcgg cgttggggct tccgctattg gtctcggtgt cgcggaaatc
     1021 cttcttgggc gccaccgttg gccttcctgt aaaggatctg ggtccagcga gccttgcggc
     1081 ggaacttcac gcgatcggca atggcgctga ctacgtccgc acccacgcgc ctggagatct
     1141 gcgaagcgca atcaccttct cggaaaccct cgcgaaattt cgcagtcgcg acgccagaga
     1201 ccgagggtta gatcatgcct agcattcacc ttccggccgc ccgctagcgg accctggtca
     1261 ggttccgcga aggtgggcgc agacatgctg ggctcgtcag gatcaaactg cactatgagg
     1321 cggcggttca taccgcgcca ggggagcgaa tggacagcga ggagcctccg aacgttcggg
     1381 tcgcctgctc gggtgatatc gacgaggttg tgcggctgat gcacgacgct gcggcgtgga
     1441 tgtccgccaa gggaacgccc gcctgggacg tcgcgcggat cgaccggaca ttcgcggaga
     1501 ccttcgtcct gagatccgag ctcctaggga tcgcctcaga aaacggaaaa taaagcacgc
     1561 taagccgtaa gtaagcgtgc tcctgtgaaa gccacagcta aaactgcgta gtacacatag
     1621 aggtattttt ttctagattt atatcgaatt tcgttatcga atttcgctta ttattgatca
     1681 actcgttgca gatcatgaag gcgaaagtat gacgaataac cctaccgatg acagcagacc
     1741 atggtcggtc tttcttattt ttctgcggct tggattgaca tcttttggcg gccccattgc
     1801 gcacttgggc tacttccgcg ccgaatttgt cacacggcgg cgctggctct ccgaacggag
     1861 ctatgctgac ttggtcgcgc tttgtcagtt cttgccaggg cctgcaagca gccaggtcgg
     1921 catagcggta ggactgtctc gggctggata cagcggggcg ctggctgctt gggctggctt
     1981 cacgctgccg tctgccatag ccttgatcct ttttgcgctc ggcatctcca gctatggcga
     2041 ttacgtctcg cagggcgcgt tgcatggctt aaaagtggtg gctgtggccg tggtcgctca
     2101 agcagtatgg ggcatggcgc gtaacctatg cacggatggg ctgcgagtca ccatcatggc
     2161 aattgctacc tgcgtcgttt tacttgtgcc gtccgcgtgg ggacaggttg gcgtgattgc
     2221 tatcgcaggc atcgcaggcc ggttattgtt caagccagcg aaagttgttg agcatgaccc
     2281 cctacctatc acggtcagtc accgggccgg cgtgctttgg ctctcgctgt tctttgtctt
     2341 gctgattggc ctgccggtgt tggccgaact gatgccaagt caaaccatgg caatggtgga
     2401 ttccttctat cgtgtcggat cactggtgtt cggcggtggt cacgttgtgc tgccattact
     2461 gcaagccgaa gtggtgccct ccggctgggt caacaatgaa tcctttctcg cggggtacgg
     2521 ggcagctcaa gcggtgcccg gccctttgtt cacgttcgcc gcgtttcttg gtgcctcgat
     2581 gaacaccgcc ccgtcgggct ggatcggcgg cattgtgtgt ctgctggcta tcttcgcgcc
     2641 ctcgttcttg ctggtcgtcg gatcaatgcc attttgggag cgtttgcgcc gcaatacagg
     2701 catccaagct gcgctggccg ggatcaatgc cgctgtagtc ggcttgctgc tggccgcgct
     2761 gtatcagcct gtatggacta gcgccatctt tcagccgcaa gacttcggct tggcattagt
     2821 tgcccttgtc gcacttatgt tctggaagct cccgccgtgg ctggttgtcc tcggtagcgg
     2881 tgcagctggg tggctgttga gcgtcgctct gtgaggcaca aaaaatgact gacaaagacc
     2941 tctacggcgg tttgatccgc ctgcacatcc ttcaccatgc agccgaggaa cctgtctttg
     3001 ggctggggat catcgaagag ctacgccgac acggctacga gatgagcgct ggcaccgtgt
     3061 acccgatgct gcacggcctg gaaaaaaaag gctatctgac ctcacgccac gaacgcaccg
     3121 ggcgacgtga acggcgtgtt tatgacatca ctgaacaagg cagaactgca cttgctgatg
     3181 cgaaaacaaa ggtcaaggag ctgttcggag agttggtaga aggtggttga gtttttgctt
     3241 ctatgtcagg ttgagctata ccctaactgg attgtcattt tcagaagacg actgcaccag
     3301 ttgattgggc gtaatggctg ttgtgcagcc agctcctgac agttcaatat cagaagtgat
     3361 ctgcaccaat ctcgactatg ctcaatactc gtgtgggctc tgttgcaaaa atcgtgaagc
     3421 ttgagcatgc ttggcggaga ttggacggac ggaacgatga cggatttcaa gtggcgccat
     3481 ttccagggtg atgtgatcct gtgggcggtg cgctggtatt gtcgctatcc gatcagctat
     3541 cgcgaccttg aggaaatgct ggcggaacgc ggcatttcgg tcgaccatac gacgatctat
     3601 cgctgggtcc agtgctacgc cccggagatg gagaagcggc tgcgctggtt ctggcggcgt
     3661 ggctttgatc cgagctggcg cctggatgaa acctacgtca aggtgcgggg caagtggacc
     3721 tacctgtacc gggcagtcga caagcggggc gacacgatcg atttctacct gtcgccgacc
     3781 cgcagcgcca aggcagcgaa gcggttcctg ggcaaggccc tgcgaggcct gaagcactgg
     3841 gaaaagcctg ccacgctcaa taccgacaaa gcgccgagct atggtgcagc gatcaccgaa
     3901 ttgaagcgcg aaggaaagct ggaccgggag acggcccacc ggcaggtgaa gtatctcaat
     3961 aacgtgatcg aggccgatca cggaaagctc aagatactga tcaagccggt gcgcggtttc
     4021 aaatcgatcc ccacggccta tgccacgatc aagggattcg aagtcatgcg agccctgcgc
     4081 aaaggacagg ctcgcccctg gtgcctgcag cccggcatca ggggcgaggt gcgccttgtg
     4141 gagagagctt ttggcattgg gccctcggcg ctgacggagg ccatgggcat gctcaaccac
     4201 catttcgcag cagccgcctg atcggcgcag agcgacagcc tacctctgac tgccgccaat
     4261 ctttgcaaca gagcccgccg tgctagtctg ctcggtgatg gtggagtgaa gccaacccgc
     4321 aatcgggtta tgaatctgca tcgcgattcg caatcagctg tctcttgagc atgtcgaact
     4381 cctgcgatgt tatctcgccg tgctcgtgct gctacaccaa cttgtccaac tcatccgcca
     4441 caccgctccc caccacctgc gatggccagg ttgtttgctg ggcgcattgg cggagtatcg
     4501 tctcgacccg ggacacccac accggcacaa ccttacggga gtgattcact gtcaaagaat
     4561 cggcccggtg ctctgacgca agtatcggga tggtcaccat ttgtaagccg tagacctgag
     4621 tggtgatcaa gacttcgata ccaccgaccg taccggtact aatcgacgac ggtcgtgttc
     4681 gtcgcctgcc gcagggactc tgcacacctc cgtttacgca tgtgcctgga ggagttggaa
     4741 atcgtcgtgt tcgggaaaca ttaaacacag gatggcagcg atctgagcca gcacatgatc
     4801 agctagctca ccatccggat cgacggccca ctgcatcgtc gcgccagcga tgaccgagtg
     4861 caggagcaac tcagctgccg caggagcacc tgggggcagt cgcttgcgga tcccctccac
     4921 caccgcgcgg ttccgctgga tcgcaagcgt gcgtagctcc ggcacctgga gctcgtacca
     4981 ggagatgaga tagttcaccg agaagtcgtt gcgagtgttc atgctccgaa cgagcacctg
     5041 caaaaattcc cagagccctt gcggccctgc gcctatcggt atcgcattca ggtaatgccg
     5101 cacctgctcg acgccgcgct ccatcatcct caccagcagc gtatcgcggt tggtgaagcg
     5161 ctggattaac gctgcgcggg agagccccac ctcctttgct actccgctga gcgtgaactc
     5221 tatgggaccg caacgcttca gcactacggt ggcggcctcg agtacctcgt catcggactt
     5281 gagcttgggg cggggcatca gtgttcacct tctgtatggg ttggggcgga ggctgtggct
     5341 gccgccgcca ttgtagcaaa ttgaagacgg agcgagagta gagccacgag ccccgcaaac
     5401 acggccgata caacgaggcc agggagcgga ccagcaaggt cgacaaacgc gccggccgca
     5461 agcataacca tgggcgaggc tgacagcatc accgccgaga ccgtgccgag tacccggccg
     5521 agaagttctg gcggcgtgcg gttgtagatg gcagcgttga gaatgggaga gactgagccg
     5581 gtcagcagtc ccacgagcgc gcccaacaac atcagcaccg gcacgcctgg caactgtgaa
     5641 agcagaagcg agcccaccgc agagccacaa aatgccaccg ccagccagtt ctgcgctgat
     5701 atccgggcgc cgaccgacgc atgaatggca atgccaagga gaccaccagc ccccatcatt
     5761 gaggagaaca gcccgagctc tgctacttgg cgtcctgcat ctacaaacag cgcaggcatg
     5821 atgacgctgc cgttggcgcc aacgatgccc acgaagatca tcactatacc aaagagaggg
     5881 cgcagcaggg gttcgctcca gagaaaagcg acgccggcgc gcatggagag agtcgccgtc
     5941 gtggtcatcg tccgagcggc acgcgcggga agcacccacg cgccgagcag acctgcaagg
     6001 acggagcaga acgccgtcag cccgagcgtt ggcgcagcgc caagcaggcc gattgcggcc
     6061 cccccaaggg ccgggccacc tagaatcgcg acgttcccga tcaccgcttt cagtgacgag
     6121 acgcgctcaa cggagagccc ggcgacgtgg ccgagtttgg gcagctcact gtcctgcgcg
     6181 gccataccgg gtgcgtcgaa cgcggcaccg agcaccacgc aagcgatcag cccagtgttc
     6241 gagagggcgc caacggcatc gagcagtggg atgctcgcca tggccacgcc gcccaccaca
     6301 cccgagatca atgcgacggg cgcgcgcccg aaccgatcga cgaggccacc accaacccac
     6361 gcgccgatga tggtcgcgat gacgctgcta gcggccgtgg cgcccgccca ggccgcgctc
     6421 tttgtatgag acaggacgaa ccatggaagc gcgagg
//
LOCUS       DAEBXN010000450         4782 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.guided.215, whole genome shotgun sequence.
ACCESSION   DAEBXN010000450 DAEBXN010000000
VERSION     DAEBXN010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4782
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.guided.215"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            159..1967
                     /gene="frdA"
                     /locus_tag="KJL99_004770"
     CDS             159..1967
                     /gene="frdA"
                     /locus_tag="KJL99_004770"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HBD4439156.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAINMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            1960..2694
                     /gene="frdB"
                     /locus_tag="KJL99_004771"
     CDS             1960..2694
                     /gene="frdB"
                     /locus_tag="KJL99_004771"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HBD4439157.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            2705..3100
                     /gene="frdC"
                     /locus_tag="KJL99_004772"
     CDS             2705..3100
                     /gene="frdC"
                     /locus_tag="KJL99_004772"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HBD4439158.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FALFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            3111..3470
                     /gene="frdD"
                     /locus_tag="KJL99_004773"
     CDS             3111..3470
                     /gene="frdD"
                     /locus_tag="KJL99_004773"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HBD4439159.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            3533..4666
                     /locus_tag="KJL99_004774"
     CDS             3533..4666
                     /locus_tag="KJL99_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HBD4439160.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
BASE COUNT         1169 a         1251 c         1282 g         1080 t
ORIGIN      
        1 gcactaaaaa aatcgatctc gtcaaatttc agacttatcc atcagactat actgttgtac
       61 ctataaagga gcagtggaat agcgttcgca gaccgtaact ttcaggcact taccctgaag
      121 tacggggctg tgggataaaa acaatctgga ggaatgtcgt gcaaaccttt caagccgatc
      181 ttgccattgt aggcgccggt ggcgcgggat tacgtgctgc aattgctgcc gcgcaggcaa
      241 atccaaatgc aaaaatcgca ctaatctcaa aagtataccc gatgcgtagc cataccgttg
      301 ctgcagaagg gggctccgcc gctgtcgcgc aggatcatga cagcttcgaa tatcactttc
      361 acgatacagt agcgggtggc gactggttgt gtgagcagga tgtcgtggat tatttcgtcc
      421 accactgccc aaccgaaatg acccaactgg aactgtgggg atgcccatgg agccgtcgcc
      481 cggatggtag cgtcaacgta cgtcgcttcg gcggcatgaa aatcgagcgc acctggttcg
      541 ccgccgataa gaccggcttc catatgctgc acacgctgtt ccagacctct ctgcaattcc
      601 cgcagatcca gcgttttgac gaacatttcg tgctggatat tctggttgat gatggtcatg
      661 ttcgcggcct ggtagcaata aacatgatgg aaggcacgtt ggtgcagatc cgtgctaacg
      721 cggtcgttat ggctaccggc ggtgcgggtc gcgtttatcg ttacaacacc aacggcggca
      781 tcgttaccgg tgacggtatg ggtatggcgc taagccacgg cgttccgctg cgtgacatgg
      841 aattcgttca gtatcaccca accggtctgc caggttccgg tatcctgatg accgaaggct
      901 gccgtggtga aggcggtatt ctggtcaaca aaaatggcta ccgttatctg caagattacg
      961 gcatgggccc ggaaactccg ctgggcgagc cgaaaaacaa atatatggaa ctgggtccac
     1021 gcgacaaagt ttctcaggcc ttctggcacg aatggcgtaa aggcaacacc atctccacgc
     1081 cgcgtggtga tgtggtttat ctcgacctgc gtcacctcgg cgagaaaaaa ctgcatgaac
     1141 gtctgccgtt catctgcgaa ctggcgaaag cgtacgttgg cgtcgatccg gttaaagaac
     1201 cgattccggt acgtccgact gcacactaca ccatgggcgg tatcgaaacc gatcagaact
     1261 gtgaaacccg cattaaaggt ctgttcgccg tgggtgaatg ttcctctgtt ggtctgcacg
     1321 gtgcaaaccg tctgggctcc aactccctgg cggaactggt ggtcttcggc cgtctggccg
     1381 gtgaacaagc gacagagcgt gcagcaactg ccggtaatgg caacgaagcg gcaattgaag
     1441 cgcaggcagc tggcgttgaa caacgtctga aagatctagt taaccaggat ggcggcgaaa
     1501 actgggcgaa gatccgcgac gaaatgggcc tggcaatgga agaaggttgc ggtatctacc
     1561 gtacgccgga actgatgcag aaaaccatcg acaagctggc agagctgcag gaacgcttca
     1621 agcgcgtgcg catcaccgac acctccagcg tgttcaacac cgacctgctc tacaccattg
     1681 aactgagcca cggtctgaac gttgctgaat gtatggcgca ctccgcaatg gcacgtaaag
     1741 agtcccgcgg cgcacaccag cgtctggacg aaggttgcac cgagcgtgac gacgtcaact
     1801 ttctcaaaca caccctcgcc ttccgcgatg ctgatggcac gactcgcctg gagtacagcg
     1861 acgtgaagat tactacgctg ccgccagcta aacgcgttta cggtggcgaa gcggatgcag
     1921 ccgataaggc ggaagcagcc aataataagg agaaggcgaa tggctgagat gaaaaacctg
     1981 aaaattgagg tggtgcgcta taacccggaa gtcgataccg caccgcatag cgcattctat
     2041 gaagtgcctt atgacgcaac tacctcatta ctggatgcgc tgggctacat caaagacaac
     2101 ctggcaccgg acctgagtta ccgctggtcc tgccgtatgg cgatttgtgg ttcctgcggc
     2161 atgatggtta acaacgtgcc aaaactggca tgtaaaacct tcctgcgtga ttacaccgac
     2221 ggtatgaagg ttgaagcgtt agctaacttc ccgattgaac gcgatctggt ggtcgatatg
     2281 acccacttca tcgaaagtct ggaagcgatc aaaccgtaca tcatcggcaa ctcccgcacc
     2341 gcggatcagg gtactaacat ccagaccccg gcgcagatgg caaagtatca ccagttctcc
     2401 ggttgcatca actgtggtct gtgctacgcc gcgtgcccgc agtttggcct gaacccagag
     2461 ttcatcggtc cggctgccat tacgctggcg catcgttata acgaagatag ccgcgaccac
     2521 ggtaagaagg agcgtatggc gcagttgaac agccagaacg gtgtatggag ctgtactttc
     2581 gtgggctatt gctccgaagt ctgcccgaaa cacgtcgatc cggctgcggc cattcagcag
     2641 ggcaaagtag aaagttcgaa agactttctt atcgcgaccc tgaaaccacg ctaaggagtg
     2701 caacatgacg actaaacgta aaccgtatgt acggccaatg acgtccacct ggtggaaaaa
     2761 attgccgttt tatcgctttt acatgctgcg cgaaggcacg gcggttccgg ctgtgtggtt
     2821 cagcattgaa ctgattttcg cgctgtttgc cctgaaaaat ggcccggaag cctgggcggg
     2881 attcgtcgac tttttacaaa acccggttat cgtgatcatt aacctgatca ctctgacggc
     2941 agctctgctg cacaccaaaa cctggtttga actggcaccg aaagcggcca atatcattgt
     3001 aaaagacgaa aaaatgggac cagagccaat tatcaaaagt ctctgggcgg taactgtggt
     3061 tgccaccatc gtaatcctgt ttgttgccct gtactggtaa ggagcctgag atgattaatc
     3121 caaatccaaa gcgttctgac gaaccggtat tctggggcct cttcggggcc ggtggtatgt
     3181 ggagcgcaat tattgcgccg gtgatgatcc tgctggtggg tattctgctg ccactggggc
     3241 tgtttccggg tgatgcgctg agctacgagc gcgttctggc gttcgcgcag agcttcattg
     3301 gtcgcgtatt cctgttcctg atgatcgttc tgccgctgtg gtgtggttta caccgtatgc
     3361 accacgcgat gcacgatctg aaaatccacg tgcctgcggg caaatgggtt ttctacggtc
     3421 tggctgctat cctgacagtt gtcacgctga ttggtgtcgt tacaatctaa cgcatcgcca
     3481 atgtaaatcc ggcccgccta tggcgggccg ttttgtatgg aaaccagacc ttatgttcaa
     3541 aacgacgctc tgcgccttat taattaccgc ctcttactcc acatttgctg cccctcaaca
     3601 aatcaacgat attgtgcatc gcacaattac cccgcttata gagcaacaaa agatccccgg
     3661 tatggcggtg gcggtaattt atcagggtaa accttattac tttacctggg gctatgcgga
     3721 catcgccaaa aagcagcccg tcacacagca aacgttgttt gagttaggtt cggtcagcaa
     3781 aacatttacg ggcgtgcttg gtggcgacgc tattgctcga ggggaaatca agttaagcga
     3841 tcccgcaaca aaatactggc ctgaacttac cgctaaacag tggaatggga tcacactatt
     3901 acatcttgcg acctacaccg ctggcggcct gccattgcag gtgccggatg aagtgaaatc
     3961 ctcaagcgac ttgctgcgct tctatcaaaa ctggcagcct gcatgggctc caggaacaca
     4021 acgtctgtat gccaactcca gtatcggttt gttcggcgca ctggctgtga agccgtctgg
     4081 tttgagtttt gagcaggcga tgcaaactcg tgtcttccag ccactcaaac tcaaccatac
     4141 gtggattaat gtacctcccc cagaagaaaa gaattacgcc tggggatatc gcgaaggtaa
     4201 ggcagtgcat gtttcgccag gggcgttaga tgctgaagct tatggtgtga agtcgaccat
     4261 tgaagatatg gcccgctggg tgcaaagcaa tttacccccc cttgatatca atgagaaaac
     4321 gcttcaacaa gggatacaac tggcacaatc tcgctactgg caaaccggcg atatgtatca
     4381 gggcctgggc tgggaaatgc tggactggcc ggtcaatcct gacagcatca ttaacggcag
     4441 tgacaataaa attgcactgg cagcacgccc ggtaaaagcg attaagcccc caactcctgc
     4501 agtacgcgca tcatgggtac ataaaacggg ggcgaccggc ggatttggta gctatgtcgc
     4561 gtttattcca gaaaaagagc tgggtatcgt gatgctggct aacaaaaact atcccaatcc
     4621 agcgagagtc gccgccgcct gtcagattct caacgctcta cagtaaaatt tcatcgggtc
     4681 cgaattttcg gaccttttct gcgcttttcc ttgctgtcat ctacacttag aaaaaaacca
     4741 gtaaggaaac atttatgcgc ctgctccctc tcgttgccgc ag
//
LOCUS       DAEBXN010000451         3916 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.guided.235, whole genome shotgun sequence.
ACCESSION   DAEBXN010000451 DAEBXN010000000
VERSION     DAEBXN010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3916
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.guided.235"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(<1..161)
                     /locus_tag="KJL99_004775"
     CDS             complement(<1..161)
                     /locus_tag="KJL99_004775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBD4439161.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFART"
     gene            complement(287..637)
                     /locus_tag="KJL99_004776"
     CDS             complement(287..637)
                     /locus_tag="KJL99_004776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HBD4439162.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            complement(840..1853)
                     /gene="intI1"
                     /locus_tag="KJL99_004777"
     CDS             complement(840..1853)
                     /gene="intI1"
                     /locus_tag="KJL99_004777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HBD4439163.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            2063..2893
                     /locus_tag="KJL99_004778"
     CDS             2063..2893
                     /locus_tag="KJL99_004778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HBD4439164.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     gene            3006..3797
                     /gene="aadA1"
                     /locus_tag="KJL99_004779"
     CDS             3006..3797
                     /gene="aadA1"
                     /locus_tag="KJL99_004779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HBD4439165.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT         1020 a         1021 c         1012 g          863 t
ORIGIN      
        1 gtgcgggcga agcttatcag cgcttccagt tgcggacgct tgacatcctt gccggatgcc
       61 ttgtcgctaa aagcgcgatc aaccttgacg ccttccagtt gccgttccgg gttctggtcg
      121 aaggtgctga ccctgatata cccaatgcgc tgtccagtca tggaattccc tgcaaaatgt
      181 cagggaagac tctatgacct tcaacgagat atgtcaataa attcaaaatt caatcctatc
      241 ctgacgcaat ttacacatgg catctgacat caggttaggg tatgcctcaa cctgacggcg
      301 gcgaatcaca agcgtccggt ttgacgctgg tttcggtcgc agtgctggcc cgcgcctgga
      361 agcgctgata gcactccagc ccgcagaaat gctccacgta ctcggcccct tccggcgtga
      421 aggcggcatc gagcgggatt tccttgcagc acacgcagca actggtgctc ggttcattgg
      481 cgttcatggt ggtgttcctc cattggttga cgaagccgac gaaggccgcc gccggcatcg
      541 gcctggcgaa caggaaaccc tgcaccgtgt cgcaacccgc ctgccgcaac cacgcaaggc
      601 agtcgggtgt ttccacaccc tcggctacca cctccattgt cgttttcaga agacggctgc
      661 actgaacgtc agaagccgac tgcactatag cagcggaggg gttggatcca tcaggcaacg
      721 acgggctgct gccggccatc agcggacgca gggaggactt tccgcaaccg gccgttcgat
      781 gcggcaccga tggccttcgc gcaggggtag tgaatccgcc aggattgact tgcgctgccc
      841 tacctctcac tagtgagggg cggcagcgca tcaagcggtg agcgcactcc ggcaccgcca
      901 actttcagca catgcgtgta aatcatcgtc gtagagacgt cggaatggcc gagcagatcc
      961 tgcacggttc gaatgtcgta accgctgcgg agcaaggccg tcgcgaacga gtggcggagg
     1021 gtgtgcggtg tggcgggctt cgtgatgcct gcttgttcta cggcacgttt gaaggcgcgc
     1081 tgaaaggtct ggtcatacat gtgatggcga cgcacgacac cgctccgtgg atcggtcgaa
     1141 tgcgtgtgct gcgcaaaaac ccagaaccac ggccaggaat gcccggcgcg cggatacttc
     1201 cgctcaaggg cgtcgggaag cgcaacgccg ctgcggccct cggcctggtc cttcagccac
     1261 catgcccgtg cacgcgacag ctgctcgcgc aggctgggtg ccaagctctc gggtaacatc
     1321 aaggcccgat ccttggagcc cttgccctcc cgcacgatga tcgtgccgtg atcgaaatcc
     1381 agatccttga cccgcagttg caaaccctca ctgatccgca tgcccgttcc atacagaagc
     1441 tgggcgaaca aacgatgctc gccttccaga aaaccgagga tgcgaaccac ttcatccggg
     1501 gtcagcacca ccggcaagcg ccgcgacggc cgaggtcttc cgatctcctg aagccagggc
     1561 agatccgtgc acagcacctt gccgtagaag aacagcaagg ccgccaatgc ctgacgatgc
     1621 gtggagaccg aaaccttgcg ctcgttcgcc agccaggaca gaaatgcctc gacttcgctg
     1681 ctgcccaagg ttgccgggtg acgcacaccg tggaaacgga tgaaggcacg aacccagtgg
     1741 acataagcct gttcggttcg taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg
     1801 tccagaacct tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt
     1861 tatgactgtt tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac
     1921 gccgtgggtc gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc
     1981 cctaaaacaa agttgggcga acccggagcc tcattaattg ttagccgtta aaattaagcc
     2041 ctttaccaaa ccaatactta ttatgaaaaa cacaatacat atcaacttcg ctattttttt
     2101 aataattgca aatattatct acagcagcgc cagtgcatca acagatatct ctactgttgc
     2161 atctccatta tttgaaggaa ctgaaggttg ttttttactt tacgatgcat ccacaaacgc
     2221 tgaaattgct caattcaata aagcaaagtg tgcaacgcaa atggcaccag attcaacttt
     2281 caagatcgca ttatcactta tggcatttga tgcggaaata atagatcaga aaaccatatt
     2341 caaatgggat aaaaccccca aaggaatgga gatctggaac agcaatcata caccaaagac
     2401 gtggatgcaa ttttctgttg tttgggtttc gcaagaaata acccaaaaaa ttggattaaa
     2461 taaaatcaag aattatctca aagattttga ttatggaaat caagacttct ctggagataa
     2521 agaaagaaac aacggattaa cagaagcatg gctcgaaagt agcttaaaaa tttcaccaga
     2581 agaacaaatt caattcctgc gtaaaattat taatcacaat ctcccagtta aaaactcagc
     2641 catagaaaac accatagaga acatgtatct acaagatctg gataatagta caaaactgta
     2701 tgggaaaact ggtgcaggat tcacagcaaa tagaacctta caaaacggat ggtttgaagg
     2761 gtttattata agcaaatcag gacataaata tgtttttgtg tccgcactta caggaaactt
     2821 ggggtcgaat ttaacatcaa gcataaaagc caagaaaaat gcgatcacca ttctaaacac
     2881 actaaattta taaaaaatct aatggcaaaa tcgcccaacc cttcaatcaa gtcgggacgg
     2941 ccaaaagcaa gcttttggct cccctcgctg gcgctcggcg ccccttattt caaacgttaa
     3001 acatcatgag ggaagtggtg atcgccgaag tatcgactca actatcagag gtagttggcg
     3061 tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg
     3121 atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg
     3181 atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag
     3241 agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt
     3301 ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg
     3361 caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa
     3421 gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg
     3481 aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact
     3541 gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa
     3601 ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc
     3661 agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct
     3721 tggcctcgcg cgcagatcag ttggaagaat ttgttcacta cgtgaaaggc gagatcacca
     3781 aggtagtcgg caaataatgt ctaacaattc gttcaagccg acgccgcttc gcggcgcggc
     3841 ttaactcaag cgttagatgc actaagcaca taattgcggt gatgctgcca acttactgat
     3901 ttagtgtatg atggtg
//
LOCUS       DAEBXN010000452         2747 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.guided.269, whole genome shotgun sequence.
ACCESSION   DAEBXN010000452 DAEBXN010000000
VERSION     DAEBXN010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.guided.269"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            complement(<1..704)
                     /locus_tag="KJL99_004780"
     CDS             complement(<1..704)
                     /locus_tag="KJL99_004780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBD4439166.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRQPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFL"
     gene            966..1424
                     /gene="gpt"
                     /locus_tag="KJL99_004781"
     CDS             966..1424
                     /gene="gpt"
                     /locus_tag="KJL99_004781"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBD4439167.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            1516..>2747
                     /gene="frsA"
                     /locus_tag="KJL99_004782"
     CDS             1516..>2747
                     /gene="frsA"
                     /locus_tag="KJL99_004782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HBD4439168.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIE"
BASE COUNT          649 a          703 c          714 g          681 t
ORIGIN      
        1 aggaagcgca ccagcagttt gttggcatta cccaggccaa cgtggatttc accgccggag
       61 tgaccgcctt tcagaccttt taaggttaac ttgaaggttt caaaaccagc tggaaccgct
      121 tcacgatcta aatgcaggtt ggaggtgaag tcgatacccc ccgcacaacc catgtagatt
      181 tcaccttctt cttcggagtc ggtgttaatc agaatatcag cctgcaacca gttgctctgt
      241 aagccgaacg caccgtccat accggcttct tcggtcatgg tcagcagcac ttccagcggg
      301 ccgtgaacca cgttttcgtc agccagaacc gccagtgcag aggccatacc aatgccgtta
      361 tccgcaccca gcgtggtgcc gcgcgcttta acccattcgc catcaatata aggctggata
      421 ggatctttcg tgaagtcatg cacggtgtcg ttatttttct gcggcaccat atcgaggtgg
      481 gcctgtaaga cgaccggttt acgattttcc atacctgcgg tagcaggttg acgaatcagg
      541 atattaccta cctgatcgcg ttcgacatgg aaacctttct cttttgccca accaacaatg
      601 tattcagcga gttgctcttc atgataggac gggtgaggaa tagaacagat tttggcaaaa
      661 atatcccaca gcggctgtgg agataattga gacagttcag acacgttaag tctccttgtc
      721 gatcacccgc aaaacagtat tgcaggtcac agggttagca gaaaatgttg tcaacacaag
      781 acaggcttgc gagatatgta ttgagaatac cactttatcc cgcgtcaggg agaggcagtg
      841 cgtaaaaaga cgcggactca tgtgaaatac tggtttttag tgcgccagat ctctataatc
      901 tcgcgcaacc tattttcccc tcgaacactt tttaagccgt agataaacag gctgggacac
      961 ttcacatgag cgaaaaatac atcgtcacct gggacatgtt gcagatccat gcacgtaaac
     1021 tcgcaagccg actgatgcct tctgaacaat ggaaaggcat tattgccgta agccgtggcg
     1081 gtctggtacc gggggcgtta ctggcgcgtg aactgggtat tcgtcatgtc gataccgttt
     1141 gtatttccag ctacgatcac gacaaccagc gcgagcttaa agtgctgaaa cgcgcagaag
     1201 gcgatggcga aggcttcatc gttattgatg acctggtgga taccggtggt actgctgttg
     1261 cgattcgtga aatgtatcca aaagcgcact ttgtcaccat cttcgcaaaa ccggctggtc
     1321 gtccgctggt tgatgactat gttgttgata tcccgcaaga tacctggatc gaacagccgt
     1381 gggatatggg cgtcgtattc gtcccgccaa tctccggtcg ctaatctttt caacgcctgg
     1441 cattgccggg cgttgttctt tttaacttca ggcaggttac aatagtttcc agtaagtatt
     1501 ctggaggctg catccatgac acaggcaaac ctgagcgaaa ccctgttcaa accccgcttt
     1561 aaacatcctg aaacctcgac gctagtccgc cgctttaatc acggcgcaca accgcctgtg
     1621 cagtcggctc ttgatggtaa aaccatccct cactggtatc gcatgattaa ccgtctgatg
     1681 tggatctggc gcggtattga cccacgtgaa atcctcgacg tccaggcacg tattgtgatg
     1741 agcgatgctg aacgtaccga cgatgattta tacgataccg tgattggcta ccgtggtggc
     1801 aactggattt atgagtgggc cacccaggcg atggtgtggc aacaaaaagc ctgtacggaa
     1861 gaagatccgc aactcagtgg tcgacactgg ctgcatgcgg ctacgttgta caacattgcc
     1921 gcctatcctc atctgaaagg agatgacctg gccgagcaag cgcaggcttt atcaaaccgc
     1981 gcctatgaag aggccgccca gcgtctgccg ggcacgatgc ggcagatgga gtttaccgta
     2041 cccggcggtg cgcccatcac cggctttttg catatgccga aaggcgatgg cccgttcccg
     2101 acagtattaa tgtgtggtgg tctggatgcg atgcagacgg actattacag cctgtatgaa
     2161 cgttattttg cgccgcgcgg cattgcgatg ctgactattg atatgccgtc ggtgggcttt
     2221 tcttcaaaat ggaagctcac ccaggactcc agcctgttgc atcagcacgt cttaaaggcg
     2281 ctgcctaacg taccgtgggt ggatcacact cgcgtcgcgg cctttggttt ccgtttcggc
     2341 gctaacgttg ccgtgcgtct ggcatacctt gaatcgccgc gtctgaaagc ggttgcctgt
     2401 cttggtccgg tagttcatac cctgttgagt gattttaagt gccagcaaca ggtgccggaa
     2461 atgtatcttg acgttctggc gagtcgtttg gggatgcatg atgcttccga cgaagcgtta
     2521 cgcgtggagc tgaatcgcta ttcattaaaa gtgcaaggat tgctggggcg tcgctgccca
     2581 acgccaatgt tatcaggcta ctggaagaac gatccgttca gcccggaaga ggactcacgc
     2641 ttaatcacct catcatctgc tgacggtaaa ttattagaga tcccatttaa cccggtgtat
     2701 cggaattttg acaaaggtct tcaggaaatc accggctgga tcgaaaa
//
LOCUS       DAEBXN010000453         2330 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.guided.281, whole genome shotgun sequence.
ACCESSION   DAEBXN010000453 DAEBXN010000000
VERSION     DAEBXN010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.guided.281"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..303
                     /locus_tag="KJL99_004783"
     CDS             <1..303
                     /locus_tag="KJL99_004783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HBD4439169.1"
                     /translation="VKIYDSLCQQQWITENGSMITLSGIQYFHEMGIDVPSKHSRKIC
                     CACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKGYAAFKTHFHI"
     gene            complement(281..904)
                     /gene="tetR(B)"
                     /locus_tag="KJL99_004784"
     CDS             complement(281..904)
                     /gene="tetR(B)"
                     /locus_tag="KJL99_004784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HBD4439170.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            986..2191
                     /gene="tet(B)"
                     /locus_tag="KJL99_004785"
     CDS             986..2191
                     /gene="tet(B)"
                     /locus_tag="KJL99_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HBD4439171.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
BASE COUNT          594 a          449 c          501 g          786 t
ORIGIN      
        1 gttaagatct atgattccct ttgtcaacag caatggatca ctgaaaatgg ttcaatgatc
       61 acattaagtg gtattcaata ttttcatgaa atgggaattg acgttccttc caaacattca
      121 cgtaaaatct gttgtgcgtg tttagattgg agtgaacgcc gtttccattt aggtgggtac
      181 gttggagccg cattattttc gctttatgaa tctaaagggt ggttaactcg acatcttggt
      241 taccgtgaag ttaccatcac ggaaaaaggt tatgctgctt ttaagaccca ctttcacatt
      301 taagttgttt ttctaatccg catatgatca attcaaggcc gaataagaag gctggctctg
      361 caccttggtg atcaaataat tcgatagctt gtcgtaataa tggcggcata ctatcagtag
      421 taggtgtttc cctttcttct ttagcgactt gatgctcttg atcttccaat acgcaaccta
      481 aagtaaaatg ccccacagcg ctgagtgcat ataatgcatt ctctagtgaa aaaccttgtt
      541 ggcataaaaa ggctaattga ttttcgagag tttcatactg tttttctgta ggccgtgtac
      601 ctaaatgtac ttttgctcca tcgcgatgac ttagtaaagc acatctaaaa cttttagcgt
      661 tattacgtaa aaaatcttgc cagctttccc cttctaaagg gcaaaagtga gtatggtgcc
      721 tatctaacat ctcaatggct aaggcgtcga gcaaagcccg cttatttttt acatgccaat
      781 acaatgtagg ctgctctaca cctagcttct gggcgagttt acgggttgtt aaaccttcga
      841 ttccgacctc attaagcagc tctaatgcgc tgttaatcac tttactttta tctaatctag
      901 acatcattaa ttcctaattt ttgttgacac tctatcattg atagagttat tttaccactc
      961 cctatcagtg atagagaaaa gtgaaatgaa tagttcgaca aagatcgcat tggtaattac
     1021 gttactcgat gccatgggga ttggccttat catgccagtc ttgccaacgt tattacgtga
     1081 atttattgct tcggaagata tcgctaacca ctttggcgta ttgcttgcac tttatgcgtt
     1141 aatgcaggtt atctttgctc cttggcttgg aaaaatgtct gaccgatttg gtcggcgccc
     1201 agtgctgttg ttgtcattaa taggcgcatc gctggattac ttattgctgg ctttttcaag
     1261 tgcgctttgg atgctgtatt taggccgttt gctttcaggg atcacaggag ctactggggc
     1321 tgtcgcggca tcggtcattg ccgataccac ctcagcttct caacgcgtga agtggttcgg
     1381 ttggttaggg gcaagttttg ggcttggttt aatagcgggg cctattattg gtggttttgc
     1441 aggagagatt tcaccgcata gtcccttttt tatcgctgcg ttgctaaata ttgtcgcttt
     1501 ccttgtggtt atgttttggt tccgtgaaac caaaaataca cgtgataata cagataccga
     1561 agtaggggtt gagacgcaat cgaattcggt atacatcact ttatttaaaa cgatgcccat
     1621 tttgttgatt atttattttt cagcgcaatt gataggccaa attcccgcaa cggtgtgggt
     1681 gctatttacc gaaaatcgtt ttggatggaa tagcatgatg gttggctttt cattagcggg
     1741 tcttggtctt ttacactcag tattccaagc ctttgtggca ggaagaatag ccactaaatg
     1801 gggcgaaaaa acggcagtac tgctcggatt tattgcagat agtagtgcat ttgccttttt
     1861 agcgtttata tctgaaggtt ggttagtttt ccctgtttta attttattgg ctggtggtgg
     1921 gatcgcttta cctgcattac agggagtgat gtctatccaa acaaagagtc atcagcaagg
     1981 tgctttacag ggattattgg tgagccttac caatgcaacc ggtgttattg gcccattact
     2041 gtttgctgtt atttataatc attcactacc aatttgggat ggctggattt ggattattgg
     2101 tttagcgttt tactgtatta ttatcctgct atcgatgacc ttcatgttaa cccctcaagc
     2161 tcaggggagt aaacaggaga caagtgctta gttatttcgt caccaaatga tgttattccg
     2221 cgaaatataa tgaccctctt gataacccaa gagggcattt tttacgataa agaagattta
     2281 gcttcaaata aaacctatct attttattta tctttcaagc tcaataaaaa
//
LOCUS       DAEBXN010000454         1559 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.guided.334, whole genome shotgun sequence.
ACCESSION   DAEBXN010000454 DAEBXN010000000
VERSION     DAEBXN010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.guided.334"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            126..986
                     /locus_tag="KJL99_004786"
     CDS             126..986
                     /locus_tag="KJL99_004786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A broad-spectrum beta-lactamase TEM-1"
                     /protein_id="HBD4439172.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     gene            1133..1414
                     /locus_tag="KJL99_004787"
                     /pseudo
     CDS             1133..1414
                     /locus_tag="KJL99_004787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
BASE COUNT          387 a          347 c          407 g          418 t
ORIGIN      
        1 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
       61 atatgtatcc gctcatgaga caataaccct ggtaaatgct tcaataatat tgaaaaagga
      121 agagtatgag tattcaacat tttcgtgtcg cccttattcc cttttttgcg gcattttgcc
      181 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
      241 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
      301 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggtgcggtat
      361 tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
      421 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
      481 aattatgcag tgctgccata accatgagtg ataacactgc tgccaactta cttctgacaa
      541 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
      601 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
      661 cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
      721 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
      781 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
      841 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
      901 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
      961 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     1021 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     1081 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccttatggc
     1141 agagcaggga aaggaattgc cgggctatgt gcaacgggaa tttgaagaat ttctccaatg
     1201 cgggcggctg gagcatggct ttctacgggt tcgctgcgag tcttgccacg ccgagcacct
     1261 ggtcgctttc agctgtaagc gtcgcggttt ctgcccgagc tgtggggcgc ggcggatggc
     1321 cgaaagtgcc gccttgctgg ttgatgaagt actgcctgaa caacccatgc gtcagtgggt
     1381 gttgagcttc ccgtttcagc tgcgtttcct gtttggggtc gtttgcggga aggggcggaa
     1441 tcctacgcta aggctttggc cagcgatatt ctccggtgag attgatgtgt tcccagggga
     1501 taggagaagt cgcttgatat ggcactgttg caaatagtcg gtggtgataa acttatcat
//
LOCUS       DAEBXN010000455         1483 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.guided.336, whole genome shotgun sequence.
ACCESSION   DAEBXN010000455 DAEBXN010000000
VERSION     DAEBXN010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.guided.336"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            <1..401
                     /locus_tag="KJL99_004788"
     CDS             <1..401
                     /locus_tag="KJL99_004788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBD4439173.1"
                     /translation="QKAASSLTLQQCHQWLLVGRKTFDSMGVLPNRKYAVVSKNGISS
                     SNENVLVFPSIENALKELSKVTDHVYVSGGGQIYNSLIEKADIIHLSTVHVEVEGDIK
                     FPIMPENFNLVFEQFFMSNINYTYQIWKKG"
     gene            532..1320
                     /locus_tag="KJL99_004789"
     CDS             532..1320
                     /locus_tag="KJL99_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA5"
                     /protein_id="HBD4439174.1"
                     /translation="MGEFFPAQVFKQLSHARAVIERHLAATLDTIHLFGSAIDGGLKP
                     DSDIDLLVTVSAAPNDSLRQALMLDLLKVSSPPGDGGTWRPLELTVVARSEVVPWRYP
                     ARRELQFGEWLRHDILSGTFEPAVLDHDLAILLTKARQHSLALLGPSAATFFEPVPKE
                     HFSKALFDTIAQWNAESDWKGDERNVVLALARIWYSASTGLIAPKDVAAAWVSERLPA
                     EHRPLICKARAAYLGSEDDDLAMRVEETAAFVRYAKATIERILR"
BASE COUNT          355 a          376 c          377 g          375 t
ORIGIN      
        1 gacaaaaggc tgcctcatcg ctaactttgc aacagtgcca tcaatggctc cttgtcggaa
       61 gaaaaacatt tgactctatg ggtgttcttc caaatcgcaa atatgcagta gtgtcaaaga
      121 acggaatttc aagctcaaat gaaaacgtcc tagtttttcc ttcaatagaa aatgctttga
      181 aagagctatc aaaagttaca gatcatgtat atgtctctgg cgggggtcaa atctataata
      241 gccttattga aaaagcagat ataattcatt tgtctactgt tcacgttgaa gtcgaaggtg
      301 atatcaaatt ccctataatg cctgagaatt tcaatttggt ttttgaacag ttttttatgt
      361 ctaatataaa ttatacatac cagatttgga aaaaaggcta acaatgcgtt gcagcaccag
      421 tcgcttcgct ccttggacag cttttaagtc gcgtctttgt ggttttgctg cgcaaaagta
      481 ttccacaaag ccgcaactta aaagctgccg ctgaacttaa cgttaggcat catgggtgaa
      541 tttttccctg cacaagtttt caagcagctg tcccacgctc gcgcggtgat cgagcgccat
      601 ctggctgcga cactggacac aatccacctg ttcggatctg cgatcgatgg agggctgaag
      661 ccggacagcg acatagactt gctcgtgacc gtcagcgccg cacctaacga ttcgctccgg
      721 caggcgctaa tgctcgattt gctgaaagtc tcatcaccgc caggcgatgg cggaacatgg
      781 cgaccgctgg agctaactgt tgtcgctcga agcgaagtag tgccttggcg ctatccggcg
      841 cggcgtgagc ttcagttcgg tgagtggctc cgccacgaca tcctttccgg aacgttcgag
      901 cctgccgttc tggatcacga tcttgcgatt ttgctgacca aggcgaggca acacagcctt
      961 gcgcttctag gcccatccgc agccacgttt ttcgagccgg tgccgaagga gcatttctcc
     1021 aaggcgcttt tcgacactat tgcccagtgg aatgcagagt cggattggaa gggtgacgag
     1081 cggaacgtcg ttcttgctct tgctcgcatt tggtacagcg cttcaactgg tctcattgct
     1141 cctaaggacg ttgctgccgc atgggtatcg gagcgtttgc ctgccgagca tcggcccctc
     1201 atctgcaagg cacgcgcggc gtacctgggt agcgaggacg acgacctagc aatgcgcgtc
     1261 gaagagacgg ccgcgttcgt tcgatatgcc aaagcaacga ttgagagaat cttgcgttga
     1321 gcggcatgtg cgaaaagtgc atcgacccgc gccgagggca tctgatgcct aactcggcgt
     1381 tcaagcggac gggctgcgcc cgccgctcaa ctatgcgtta gatgcactaa gcacataatt
     1441 gctcacagcc aaactatcag gtcaagtctg cttttattat ttt
//
LOCUS       DAEBXN010000456         1090 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.guided.359, whole genome shotgun sequence.
ACCESSION   DAEBXN010000456 DAEBXN010000000
VERSION     DAEBXN010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.guided.359"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            110..1015
                     /locus_tag="KJL99_004790"
     CDS             110..1015
                     /locus_tag="KJL99_004790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(A) family macrolide 2'-phosphotransferase"
                     /protein_id="HBD4439175.1"
                     /translation="MTVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVD
                     DGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA
                     MVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVA
                     DDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMI
                     DWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVT
                     YALFALDSGNEEYLAAAKAQLAAAE"
BASE COUNT          192 a          349 c          347 g          202 t
ORIGIN      
        1 agacaaaagg ctgcctcatc gctaactttg caacagtgcc ggattgaata taaccgacgt
       61 gactgttaca tttaggtggc taaacccgtc aagccctcag gagtgaatca tgaccgtagt
      121 cacgaccgcc gatacctccc aactgtacgc acttgcagcc cgacatgggc tcaagctcca
      181 tggcccgctg actgtcaatg agcttgggct cgactatagg atcgtgatcg ccaccgtcga
      241 cgatggacgt cggtgggtgc tgcgcatccc gcgccgagcc gaggtaagcg cgaaggtcga
      301 accagaggcg cgggtgctgg caatgctcaa gaatcgcctg ccgttcgcgg tgccggactg
      361 gcgcgtggcc aacgccgagc tcgttgccta tcccatgctc gaagactcga ctgcgatggt
      421 catccagcct ggttcgtcca cgcccgactg ggtcgtgccg caggactcgg aggtcttcgc
      481 ggagagcttc gcgaccgcgc tcgccgccct gcatgccgtc cccatttccg ccgccgtgga
      541 tgcggggatg ctcatccgta caccgacgca ggcccgtcag aaggtggccg acgacgttga
      601 ccgcgtccga cgcgagttcg tggtgaacga caagcgcctc caccggtggc agcgctggct
      661 cgacgacgat tcgtcgtggc cagatttctc cgtggtggtg catggcgatc tctacgtggg
      721 ccatgtgctc atcgacaaca cggagcgcgt cagcgggatg atcgactgga gcgaggcccg
      781 cgttgatgac cctgccatcg acatggccgc gcaccttatg gtctttggtg aagaggggct
      841 cgcgaagctc ctcctcacgt atgaagcggc cggtggccgg gtgtggccgc ggctcgccca
      901 ccacatcgcg gagcgccttg cgttcggggc ggtcacctac gcactcttcg ccctcgactc
      961 gggtaacgaa gagtacctcg ctgcggcgaa ggcgcagctc gccgcagcgg aatgagcgaa
     1021 cgtcgatata gcccgctcgc gacgctgttc gcggcgacct ttctcttccg gatcggcaac
     1081 gcggtggcgg
//
LOCUS       DAEBXN010000457         1051 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.guided.364, whole genome shotgun sequence.
ACCESSION   DAEBXN010000457 DAEBXN010000000
VERSION     DAEBXN010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.guided.364"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            263..922
                     /locus_tag="KJL99_004791"
     CDS             263..922
                     /locus_tag="KJL99_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HBD4439176.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
BASE COUNT          290 a          227 c          245 g          289 t
ORIGIN      
        1 caccatcata cactaaatca gtaagttggc agcatcaccc gacgcacttt gcgccgaata
       61 aatacctgtg acggaagatc acttcgcaga ataaataaat cctggtgtcc ctgttgatac
      121 cgggaagccc tgggccaact tttggcgaaa atgagacgtt gatcggcacg taagaggttc
      181 caactttcac cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt
      241 ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat
      301 atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta
      361 taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca
      421 caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt
      481 ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac
      541 cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt
      601 ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta
      661 tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt
      721 caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat
      781 gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca
      841 tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga
      901 tgagtggcag ggcggggcgt aattttttta aggcagttat tggtgccctt aaacgcctgg
      961 ttgctacgcc tgaataagtg ataataagcg gatgaatggc agaaattcga aagcaaattc
     1021 gacccggtcg tcggttcagg gcagggtcgt t
//
LOCUS       DAEBXN010000458          972 bp    DNA     linear   BCT 20-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 2a strain SF058948
            SAMN17128982-rid12250663.guided.368, whole genome shotgun sequence.
ACCESSION   DAEBXN010000458 DAEBXN010000000
VERSION     DAEBXN010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17128982
            Sequence Read Archive: SRR13282496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042347.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042347.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 23:25:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,793
            CDSs (total)                      :: 4,698
            Genes (coding)                    :: 4,279
            CDSs (with protein)               :: 4,279
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 419
            CDSs (without protein)            :: 419
            Pseudo Genes (ambiguous residues) :: 0 of 419
            Pseudo Genes (frameshifted)       :: 190 of 419
            Pseudo Genes (incomplete)         :: 128 of 419
            Pseudo Genes (internal stop)      :: 149 of 419
            Pseudo Genes (multiple problems)  :: 47 of 419
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..972
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17128982-rid12250663.guided.368"
                     /strain="SF058948"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2017-04"
                     /collected_by="LSPQ"
     gene            19..756
                     /gene="erm(B)"
                     /locus_tag="KJL99_004792"
     CDS             19..756
                     /gene="erm(B)"
                     /locus_tag="KJL99_004792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HBD4439177.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPRVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            701..871
                     /locus_tag="KJL99_004793"
     CDS             701..871
                     /locus_tag="KJL99_004793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine methyltransferase"
                     /protein_id="HBD4439178.1"
                     /translation="MSKYCLFLIVIYYLTGGNNSMSRFCKFGKLHVTKGNVDKLLGIL
                     LTASKELKRSLD"
BASE COUNT          362 a          167 c          159 g          284 t
ORIGIN      
        1 acttcaggag taaattacat gaacaaaaat ataaaatatt ctcaaaactt tttaacgagt
       61 gaaaaagtac tcaaccaaat aataaaacaa ttgaatttaa aagaaaccga taccgtttac
      121 gaaattggaa caggtaaagg gcatttaacg acgaaactgg ctaaaataag taaacaggta
      181 acgtctattg aattagacag tcatctattc aacttatcgt cagaaaaatt aaaactgaat
      241 actcgtgtca ctttaattca ccaagatatt ctacagtttc aattccctaa caaacagagg
      301 tataaaattg ttgggaatat tccttaccat ttaagcacac aaattattaa aaaagtggtt
      361 tttgaaagcc atgcgtctga catctatctg attgttgaag aaggattcta caagcgtacc
      421 ttggatattc accgaacact agggttgctc ttgcacactc aagtctcgat tcagcaattg
      481 cttaagctgc cagcggaatg ctttcatcct aaaccaagag taaacagtgt cttaataaaa
      541 cttacccgcc ataccacaga tgttccagat aaatattgga agctatatac gtactttgtt
      601 tcaaaatggg tcaatcgaga atatcgtcaa ctgtttacta aaaatcagtt tcatcaagca
      661 atgaaacacg ccaaagtaaa caatttaagt accgttactt atgagcaagt attgtctatt
      721 tttaatagtt atctattatt taacgggagg aaataattct atgagtcgct tttgtaaatt
      781 tggaaagtta cacgttacta aagggaatgt agataaatta ttaggtatac tactgacagc
      841 ttccaaggag ctaaagaggt ccctagacta gcaagaagta cacaagaagc cttaaagatt
      901 atagaaaagc ttttccatga aatgcttaac attatttaat gttaacatgt ggctttgtgg
      961 taactaccac ga
//
